LOCUS       DAZGVQ010000001        95651 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000001 DAZGVQ010000000
VERSION     DAZGVQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 95651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.001"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            49..567
                     /gene="yfgH"
                     /locus_tag="ACHLAG_000001"
     CDS             49..567
                     /gene="yfgH"
                     /locus_tag="ACHLAG_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP0653315.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAVGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            583..1122
                     /gene="yfgI"
                     /locus_tag="ACHLAG_000002"
     CDS             583..1122
                     /gene="yfgI"
                     /locus_tag="ACHLAG_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFP0653316.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1217..2794)
                     /gene="guaA"
                     /locus_tag="ACHLAG_000003"
     CDS             complement(1217..2794)
                     /gene="guaA"
                     /locus_tag="ACHLAG_000003"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP0653317.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2863..4329)
                     /gene="guaB"
                     /locus_tag="ACHLAG_000004"
     CDS             complement(2863..4329)
                     /gene="guaB"
                     /locus_tag="ACHLAG_000004"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFP0653318.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4491..5861
                     /gene="xseA"
                     /locus_tag="ACHLAG_000005"
     CDS             4491..5861
                     /gene="xseA"
                     /locus_tag="ACHLAG_000005"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFP0653319.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5858..6073)
                     /gene="yfgJ"
                     /locus_tag="ACHLAG_000006"
     CDS             complement(5858..6073)
                     /gene="yfgJ"
                     /locus_tag="ACHLAG_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP0653320.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6142..7614)
                     /gene="der"
                     /locus_tag="ACHLAG_000007"
     CDS             complement(6142..7614)
                     /gene="der"
                     /locus_tag="ACHLAG_000007"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFP0653321.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7732..8910)
                     /gene="bamB"
                     /locus_tag="ACHLAG_000008"
     CDS             complement(7732..8910)
                     /gene="bamB"
                     /locus_tag="ACHLAG_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFP0653322.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8921..9541)
                     /gene="yfgM"
                     /locus_tag="ACHLAG_000009"
     CDS             complement(8921..9541)
                     /gene="yfgM"
                     /locus_tag="ACHLAG_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFP0653323.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9559..10833)
                     /gene="hisS"
                     /locus_tag="ACHLAG_000010"
     CDS             complement(9559..10833)
                     /gene="hisS"
                     /locus_tag="ACHLAG_000010"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFP0653324.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10944..12062)
                     /gene="ispG"
                     /locus_tag="ACHLAG_000011"
                     /gene_synonym="gcpE"
     CDS             complement(10944..12062)
                     /gene="ispG"
                     /locus_tag="ACHLAG_000011"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFP0653325.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12089..13102)
                     /gene="rodZ"
                     /locus_tag="ACHLAG_000012"
     CDS             complement(12089..13102)
                     /gene="rodZ"
                     /locus_tag="ACHLAG_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFP0653326.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13387..14541)
                     /gene="rlmN"
                     /locus_tag="ACHLAG_000013"
     CDS             complement(13387..14541)
                     /gene="rlmN"
                     /locus_tag="ACHLAG_000013"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFP0653327.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14691..15122)
                     /gene="ndk"
                     /locus_tag="ACHLAG_000014"
     CDS             complement(14691..15122)
                     /gene="ndk"
                     /locus_tag="ACHLAG_000014"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFP0653328.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15271..17583)
                     /gene="pbpC"
                     /locus_tag="ACHLAG_000015"
     CDS             complement(15271..17583)
                     /gene="pbpC"
                     /locus_tag="ACHLAG_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFP0653329.1"
                     /translation="MPRLLTKRGYWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAKLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17584..22545)
                     /gene="yfhM"
                     /locus_tag="ACHLAG_000016"
     CDS             complement(17584..22545)
                     /gene="yfhM"
                     /locus_tag="ACHLAG_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFP0653330.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGTAEDLLIVRP"
     gene            22752..23597
                     /gene="sseA"
                     /locus_tag="ACHLAG_000017"
     CDS             22752..23597
                     /gene="sseA"
                     /locus_tag="ACHLAG_000017"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFP0653331.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23933..24058
                     /gene="timP"
                     /locus_tag="ACHLAG_000018"
     CDS             23933..24058
                     /gene="timP"
                     /locus_tag="ACHLAG_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFP0653332.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24090..24866)
                     /gene="sseB"
                     /locus_tag="ACHLAG_000019"
     CDS             complement(24090..24866)
                     /gene="sseB"
                     /locus_tag="ACHLAG_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFP0653333.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKSVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(25008..26291)
                     /gene="pepB"
                     /locus_tag="ACHLAG_000020"
     CDS             complement(25008..26291)
                     /gene="pepB"
                     /locus_tag="ACHLAG_000020"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFP0653334.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26469..26669)
                     /gene="iscX"
                     /locus_tag="ACHLAG_000021"
     CDS             complement(26469..26669)
                     /gene="iscX"
                     /locus_tag="ACHLAG_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFP0653335.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26681..27016)
                     /gene="fdx"
                     /locus_tag="ACHLAG_000022"
     CDS             complement(26681..27016)
                     /gene="fdx"
                     /locus_tag="ACHLAG_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFP0653336.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27018..28868)
                     /gene="hscA"
                     /locus_tag="ACHLAG_000023"
     CDS             complement(27018..28868)
                     /gene="hscA"
                     /locus_tag="ACHLAG_000023"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFP0653337.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNQLIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28885..29400)
                     /gene="hscB"
                     /locus_tag="ACHLAG_000024"
     CDS             complement(28885..29400)
                     /gene="hscB"
                     /locus_tag="ACHLAG_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFP0653338.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29496..29819)
                     /gene="iscA"
                     /locus_tag="ACHLAG_000025"
     CDS             complement(29496..29819)
                     /gene="iscA"
                     /locus_tag="ACHLAG_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFP0653339.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29836..30222)
                     /gene="iscU"
                     /locus_tag="ACHLAG_000026"
     CDS             complement(29836..30222)
                     /gene="iscU"
                     /locus_tag="ACHLAG_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFP0653340.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30250..31464)
                     /gene="iscS"
                     /locus_tag="ACHLAG_000027"
     CDS             complement(30250..31464)
                     /gene="iscS"
                     /locus_tag="ACHLAG_000027"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFP0653341.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31576..32064)
                     /gene="iscR"
                     /locus_tag="ACHLAG_000028"
     CDS             complement(31576..32064)
                     /gene="iscR"
                     /locus_tag="ACHLAG_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFP0653342.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32365..33102)
                     /gene="trmJ"
                     /locus_tag="ACHLAG_000029"
     CDS             complement(32365..33102)
                     /gene="trmJ"
                     /locus_tag="ACHLAG_000029"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFP0653343.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33221..34024
                     /gene="suhB"
                     /locus_tag="ACHLAG_000030"
     CDS             33221..34024
                     /gene="suhB"
                     /locus_tag="ACHLAG_000030"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFP0653344.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34169..35023
                     /gene="yfhR"
                     /locus_tag="ACHLAG_000031"
     CDS             34169..35023
                     /gene="yfhR"
                     /locus_tag="ACHLAG_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0653345.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGRGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35214..36494
                     /gene="csiE"
                     /locus_tag="ACHLAG_000032"
     CDS             35214..36494
                     /gene="csiE"
                     /locus_tag="ACHLAG_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFP0653346.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36486..37625)
                     /gene="hcaT"
                     /locus_tag="ACHLAG_000033"
     CDS             complement(36486..37625)
                     /gene="hcaT"
                     /locus_tag="ACHLAG_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFP0653347.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37785..38675)
                     /gene="hcaR"
                     /locus_tag="ACHLAG_000034"
     CDS             complement(37785..38675)
                     /gene="hcaR"
                     /locus_tag="ACHLAG_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFP0653348.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38811..40172
                     /gene="hcaE"
                     /locus_tag="ACHLAG_000035"
     CDS             38811..40172
                     /gene="hcaE"
                     /locus_tag="ACHLAG_000035"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFP0653349.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40169..40687
                     /gene="hcaF"
                     /locus_tag="ACHLAG_000036"
     CDS             40169..40687
                     /gene="hcaF"
                     /locus_tag="ACHLAG_000036"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFP0653350.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40687..41007
                     /gene="hcaC"
                     /locus_tag="ACHLAG_000037"
     CDS             40687..41007
                     /gene="hcaC"
                     /locus_tag="ACHLAG_000037"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFP0653351.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41004..41816
                     /gene="hcaB"
                     /locus_tag="ACHLAG_000038"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41004..41816
                     /gene="hcaB"
                     /locus_tag="ACHLAG_000038"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFP0653352.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41826..43028
                     /gene="hcaD"
                     /locus_tag="ACHLAG_000039"
     CDS             41826..43028
                     /gene="hcaD"
                     /locus_tag="ACHLAG_000039"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFP0653353.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43125..43547
                     /gene="yphA"
                     /locus_tag="ACHLAG_000040"
     CDS             43125..43547
                     /gene="yphA"
                     /locus_tag="ACHLAG_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP0653354.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43595..44467)
                     /gene="yphB"
                     /locus_tag="ACHLAG_000041"
     CDS             complement(43595..44467)
                     /gene="yphB"
                     /locus_tag="ACHLAG_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFP0653355.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44479..45573)
                     /gene="yphC"
                     /locus_tag="ACHLAG_000042"
     CDS             complement(44479..45573)
                     /gene="yphC"
                     /locus_tag="ACHLAG_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP0653356.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45606..46604)
                     /gene="yphD"
                     /locus_tag="ACHLAG_000043"
     CDS             complement(45606..46604)
                     /gene="yphD"
                     /locus_tag="ACHLAG_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0653357.1"
                     /translation="MSASSLSLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46629..48140)
                     /gene="yphE"
                     /locus_tag="ACHLAG_000044"
     CDS             complement(46629..48140)
                     /gene="yphE"
                     /locus_tag="ACHLAG_000044"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP0653358.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTDSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(48163..49146)
                     /gene="yphF"
                     /locus_tag="ACHLAG_000045"
     CDS             complement(48163..49146)
                     /gene="yphF"
                     /locus_tag="ACHLAG_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP0653359.1"
                     /translation="MPKKMRTTRNLLLMGTLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49243..52524)
                     /gene="yphG"
                     /locus_tag="ACHLAG_000046"
     CDS             complement(49243..52524)
                     /gene="yphG"
                     /locus_tag="ACHLAG_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFP0653360.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVVLDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52642..53835
                     /gene="yphH"
                     /locus_tag="ACHLAG_000047"
     CDS             52642..53835
                     /gene="yphH"
                     /locus_tag="ACHLAG_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP0653361.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPKGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(53899..55152)
                     /gene="glyA"
                     /locus_tag="ACHLAG_000048"
     CDS             complement(53899..55152)
                     /gene="glyA"
                     /locus_tag="ACHLAG_000048"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFP0653362.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55480..56670
                     /gene="hmpA"
                     /locus_tag="ACHLAG_000049"
     CDS             55480..56670
                     /gene="hmpA"
                     /locus_tag="ACHLAG_000049"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFP0653363.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56715..57053)
                     /gene="glnB"
                     /locus_tag="ACHLAG_000050"
     CDS             complement(56715..57053)
                     /gene="glnB"
                     /locus_tag="ACHLAG_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFP0653364.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57114..58448)
                     /gene="glrR"
                     /locus_tag="ACHLAG_000051"
     CDS             complement(57114..58448)
                     /gene="glrR"
                     /locus_tag="ACHLAG_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFP0653365.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58438..59151)
                     /gene="qseG"
                     /locus_tag="ACHLAG_000052"
     CDS             complement(58438..59151)
                     /gene="qseG"
                     /locus_tag="ACHLAG_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFP0653366.1"
                     /translation="MRHIFQRLMPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(59316..60743)
                     /gene="qseE"
                     /locus_tag="ACHLAG_000053"
     CDS             complement(59316..60743)
                     /gene="qseE"
                     /locus_tag="ACHLAG_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFP0653367.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61301..65188)
                     /gene="purL"
                     /locus_tag="ACHLAG_000054"
                     /gene_synonym="purI"
     CDS             complement(61301..65188)
                     /gene="purL"
                     /locus_tag="ACHLAG_000054"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFP0653368.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKVLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65446..67002
                     /gene="mltF"
                     /locus_tag="ACHLAG_000055"
     CDS             65446..67002
                     /gene="mltF"
                     /locus_tag="ACHLAG_000055"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFP0653369.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(66999..67502)
                     /gene="tadA"
                     /locus_tag="ACHLAG_000056"
     CDS             complement(66999..67502)
                     /gene="tadA"
                     /locus_tag="ACHLAG_000056"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFP0653370.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(67560..68195)
                     /gene="yfhb"
                     /locus_tag="ACHLAG_000057"
     CDS             complement(67560..68195)
                     /gene="yfhb"
                     /locus_tag="ACHLAG_000057"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFP0653371.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68404..69252
                     /gene="yfhH"
                     /locus_tag="ACHLAG_000058"
     CDS             68404..69252
                     /gene="yfhH"
                     /locus_tag="ACHLAG_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP0653372.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            69308..69568
                     /gene="yfhL"
                     /locus_tag="ACHLAG_000059"
     CDS             69308..69568
                     /gene="yfhL"
                     /locus_tag="ACHLAG_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFP0653373.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(69762..69842)
                     /gene="shoB"
                     /locus_tag="ACHLAG_000060"
     CDS             complement(69762..69842)
                     /gene="shoB"
                     /locus_tag="ACHLAG_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFP0653374.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(70262..70642)
                     /gene="acpS"
                     /locus_tag="ACHLAG_000061"
     CDS             complement(70262..70642)
                     /gene="acpS"
                     /locus_tag="ACHLAG_000061"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP0653375.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(70642..71373)
                     /gene="pdxJ"
                     /locus_tag="ACHLAG_000062"
     CDS             complement(70642..71373)
                     /gene="pdxJ"
                     /locus_tag="ACHLAG_000062"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFP0653376.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(71385..72113)
                     /gene="recO"
                     /locus_tag="ACHLAG_000063"
     CDS             complement(71385..72113)
                     /gene="recO"
                     /locus_tag="ACHLAG_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFP0653377.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(72125..73030)
                     /gene="era"
                     /locus_tag="ACHLAG_000064"
     CDS             complement(72125..73030)
                     /gene="era"
                     /locus_tag="ACHLAG_000064"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFP0653378.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(73027..73707)
                     /gene="rnc"
                     /locus_tag="ACHLAG_000065"
     CDS             complement(73027..73707)
                     /gene="rnc"
                     /locus_tag="ACHLAG_000065"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFP0653379.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(73980..74954)
                     /gene="lepB"
                     /locus_tag="ACHLAG_000066"
     CDS             complement(73980..74954)
                     /gene="lepB"
                     /locus_tag="ACHLAG_000066"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP0653380.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(74970..76769)
                     /gene="lepA"
                     /locus_tag="ACHLAG_000067"
     CDS             complement(74970..76769)
                     /gene="lepA"
                     /locus_tag="ACHLAG_000067"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFP0653381.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(76967..77446)
                     /gene="rseC"
                     /locus_tag="ACHLAG_000068"
     CDS             complement(76967..77446)
                     /gene="rseC"
                     /locus_tag="ACHLAG_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFP0653382.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(77443..78399)
                     /gene="rseB"
                     /locus_tag="ACHLAG_000069"
     CDS             complement(77443..78399)
                     /gene="rseB"
                     /locus_tag="ACHLAG_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFP0653383.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(78399..79049)
                     /gene="rseA"
                     /locus_tag="ACHLAG_000070"
     CDS             complement(78399..79049)
                     /gene="rseA"
                     /locus_tag="ACHLAG_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFP0653384.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(79082..79657)
                     /gene="rpoE"
                     /locus_tag="ACHLAG_000071"
     CDS             complement(79082..79657)
                     /gene="rpoE"
                     /locus_tag="ACHLAG_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFP0653385.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(79654..79809)
                     /gene="rseD"
                     /locus_tag="ACHLAG_000072"
     CDS             complement(79654..79809)
                     /gene="rseD"
                     /locus_tag="ACHLAG_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFP0653386.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            80065..81687
                     /gene="nadB"
                     /locus_tag="ACHLAG_000073"
     CDS             80065..81687
                     /gene="nadB"
                     /locus_tag="ACHLAG_000073"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFP0653387.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRIIHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(81672..82409)
                     /gene="trmN"
                     /locus_tag="ACHLAG_000074"
     CDS             complement(81672..82409)
                     /gene="trmN"
                     /locus_tag="ACHLAG_000074"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFP0653388.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            82541..83875
                     /gene="srmB"
                     /locus_tag="ACHLAG_000075"
     CDS             82541..83875
                     /gene="srmB"
                     /locus_tag="ACHLAG_000075"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFP0653389.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(84084..84965)
                     /gene="yfiE"
                     /locus_tag="ACHLAG_000076"
     CDS             complement(84084..84965)
                     /gene="yfiE"
                     /locus_tag="ACHLAG_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0653390.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
     gene            85068..85655
                     /gene="eamB"
                     /locus_tag="ACHLAG_000077"
     CDS             85068..85655
                     /gene="eamB"
                     /locus_tag="ACHLAG_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFP0653391.1"
                     /translation="MTPPLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMTLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(85711..86094)
                     /gene="grcA"
                     /locus_tag="ACHLAG_000078"
     CDS             complement(85711..86094)
                     /gene="grcA"
                     /locus_tag="ACHLAG_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFP0653392.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            86399..87088
                     /gene="ung"
                     /locus_tag="ACHLAG_000079"
     CDS             86399..87088
                     /gene="ung"
                     /locus_tag="ACHLAG_000079"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP0653393.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(87136..88173)
                     /gene="yfiF"
                     /locus_tag="ACHLAG_000080"
     CDS             complement(87136..88173)
                     /gene="yfiF"
                     /locus_tag="ACHLAG_000080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP0653394.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            88380..88799
                     /gene="trxC"
                     /locus_tag="ACHLAG_000081"
     CDS             88380..88799
                     /gene="trxC"
                     /locus_tag="ACHLAG_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFP0653395.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNRQVVDMLNGAVPKAPFDSWLNESL"
     gene            88868..89566
                     /gene="tapT"
                     /locus_tag="ACHLAG_000082"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             88868..89566
                     /gene="tapT"
                     /locus_tag="ACHLAG_000082"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFP0653396.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            89598..92258
                     /gene="pat"
                     /locus_tag="ACHLAG_000083"
     CDS             89598..92258
                     /gene="pat"
                     /locus_tag="ACHLAG_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFP0653397.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSHNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQELIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            92372..93727
                     /gene="pssA"
                     /locus_tag="ACHLAG_000084"
     CDS             92372..93727
                     /gene="pssA"
                     /locus_tag="ACHLAG_000084"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFP0653398.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMTQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            93773..94096
                     /gene="yfiM"
                     /locus_tag="ACHLAG_000085"
     CDS             93773..94096
                     /gene="yfiM"
                     /locus_tag="ACHLAG_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFP0653399.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(94093..95391)
                     /gene="kgtP"
                     /locus_tag="ACHLAG_000086"
     CDS             complement(94093..95391)
                     /gene="kgtP"
                     /locus_tag="ACHLAG_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFP0653400.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
BASE COUNT        22798 a        26078 c        24043 g        22732 t
ORIGIN      
        1 tgatgatctt aaatgtctta tttttcgtaa tgtgtataac aaggaatagt gatgaaattt
       61 aaaaaatgtc ttctgcctgt ggcaatgtta gcgtcattca ctctggcagg atgccagtca
      121 aatgctgacg atcatgccgc cgatgtttat caaaccgatc aactgaatac caaacaagaa
      181 actaaaaccg ttaatattat ttccattctt cccgcaaaag ttgccgtaga caactcccaa
      241 aataaacgga acgcacaagc cttcggcgcg cttattggcg cagtcgctgg cggtgttatc
      301 ggccacaacg tcggttctgg cagcaattcc ggaacgacgg caggggcagt tggcggcgga
      361 gctgtaggcg cggcagtggg ttctatggtg aatgataaaa ccttagtgga aggtgtttct
      421 ttaacatata aggaaggcac caaagtgtat acctctaccc aggtgggtaa agagtgccag
      481 tttacgacag gtttagccgt tgttattacc acaacgtata acgaaacgcg tattcagcca
      541 aatactaaat gtcctgaaaa gagctaataa tcaggaggag tcatgaagaa agtttttctt
      601 tgcgccatct tggcctcctt aagctatccg gctatcgcct catcattgca ggatcaactc
      661 tctgctgtag cagaagctga acagcaaggt aaaaatgaag agcaaaggca gcatgacgaa
      721 tgggtcgcgg agcgcaacag ggaaatccag caagagaagc aacgtcgcgc aaatgcccag
      781 gccgccgcta acaaaagagc ggcaacggca gcggcaaata agaaagctcg tcaggataaa
      841 ctggacgccg aagcctctgc ggacaaaaaa cgcgatcaaa gttatgaaga tgagctacgc
      901 agcttagaga ttcagaaaca aaaactggcg ctggcaaaag aagaagcccg cgttaagcga
      961 gaaaacgaat ttatcgatca ggaactgaag cacaaagctg cgcaaaccga tgtggtgcaa
     1021 tctgaagctg acgccaacag aaatatgact gaaggcggtc gcgatctgat gaaaagcgtg
     1081 ggcaaagcag aagagaacaa atcggacagc tggtttaatt aatcgatgtt agtaacttca
     1141 agcctatgat tcttgaagat aaaaaaccct ctgtagtaac agagggtttt gttcattcat
     1201 agtgcaggga ttaaaatcat tcccactcaa tggtagctgg cggcttgccg ctgatgtcat
     1261 acaccacgcg ggaaataccg ttcacttcat tgataatgcg gttggaaacg cgaccgagga
     1321 aatcgtacgg cagatgcgcc cagtgtgcgg tcataaagtc gatggtttcg acagcacgca
     1381 gagagacaac ccagtcatac ttacgaccat cgcccattac gccaacggaa cgtaccggca
     1441 ggaacacagt gaacgcctgg ctgactttgt cgtacaggtc cgctttacgc agttcttcaa
     1501 tgaagatggc gtcagcacgg cgcagcaggt cacagtactc tttcttcact tcacccagca
     1561 cacgaacgcc aaggcctggt cccgggaacg ggtgacggta cagcatgtcg tacggcaggc
     1621 ccagctccag accaatctta cgcacttcgt ctttgaacag ctctttcagc ggttcaacca
     1681 ggcccatctt catctctttc ggcaggccgc ccacgttgtg gtgagatttg atgacgtgtg
     1741 ctttaccggt tgcagaagcc gcagattcga taacgtcagg gtagatggtg ccctgcgcca
     1801 gccacttcac gtcttccagt ttcagcgctt cttcatcgaa tacttcaacg aaaacgcgcc
     1861 cgatgatttt acgttttgct tccggatcgt tttcgccagc cagcgctgac aggaagcgat
     1921 cttctgccgg tacgtgaaca atgttaagac caaagtgatc gccaaacata tccagaacct
     1981 gctctgcttc gttgagacgc agcaggccgt tgtcgacgaa tacgcaagtc aggtttttac
     2041 cgatagcgcg gtgcagcagc attgcggtta cggaggaatc cacaccacca gagaggccga
     2101 ggatgacttt atcgtcgcct acctgctcgc ggatgcgagc tacagcatcg tcgataattt
     2161 tcgctggcgt ccacagggct tcacactggc agatatcacg cacaaaacgc tccagcatgc
     2221 gcataccctg gcgggtgtga gtcacttccg ggtggaactg tacgccatag aagcgttttt
     2281 cttcgttagc cataatggca aacgggcagc tttcggtgct ggctacggtg acgaagtcgg
     2341 acgggatagc ggtaacttta tcgccgtggc tcatccagac atcgagcagc ggtttaccgt
     2401 ctgcggtcag cgcatcttcg ataccgcgaa ccagtgcgct gtcgtttacg acttcaacct
     2461 gcgcgtagcc aaattcacgt tcgttagagg cttcaacgtg accgcccaac tgcattgcca
     2521 tggtttgcat gccatagcaa acgccgaata ccggtacgcc tgcttcaaag acatactgcg
     2581 gcgcacgcgg gctgttttct tcggtggtgc tttccgggcc gccggaaaga ataatgccgc
     2641 ttggattgaa gtcacgaatt tgtgcttctg tcacatccca cgcccacagt tcgcagtaaa
     2701 cacccagctc acgcacgcgg cgcgcaacca gttgagtgta ctgagaaccg aagtccagaa
     2761 tgaggatgcg atgcttatga atgttttccg tcattgacgc ttattccgag gcaagtgaaa
     2821 cagataatat aaatcgcccg acatgaagtc gggcgaagag aatcaggagc ccagacggta
     2881 gttcggggac tctttagtaa tggtcacgtc gtgaacgtgg ctttcctgaa tgcccgcacc
     2941 gctgatacgt acaaactccg ctttagtacg cagttcgtcg atagtaccac agccggtcag
     3001 acccatacag gagcgcaggc cgcccatctg ctggtgaatg atctctttca ggcgaccttt
     3061 ataagctacg cgaccttcga taccttccgg caccagtttg tcggcagcgt tatcgctctg
     3121 gaaataacgg tcagaggaac ctttggacat cgcgcccagg gaacccatac cacggtaaga
     3181 tttgtaagaa cggccctggt agagttcgat ttcacccgga gattcttcag tacccgccag
     3241 catggaaccc accattaccg cgcttgcgcc agcggcgata gctttggcga tgtcgccgga
     3301 gaagcgaata ccaccatctg cgataaccgg aataccggtg ccttccaggg cttcaactgc
     3361 gtcagcaacg gcagtgatct gcggaacacc tacgccagta acgatacgag tcgtacagat
     3421 agaaccaggg ccgataccga ctttaaccgc gctgcaacca gcttctgcca aagcgcgtgc
     3481 acctgcagct gttgccacgt tgccgccgat gatttgcaga tccggatatt tagcacgggt
     3541 ttcacggata cgctgcaaaa cgccttctga gtgaccgtgg gaggagtcga tcagcagaac
     3601 gtcaacgcca gcggcaacca gcgcgtcaac acgctcttcg ttacccgcac ctgcgccaac
     3661 tgcagcacca acacgcagac ggccttgctc gtctttacag gcgttcggtt tacgttccgc
     3721 tttctggaag tctttcacgg tgatcatgcc gatcaggtgg aattcgtcat caaccaccag
     3781 cgctttttca acgcgttttt cgtgcatttt tgccagcacc acttcacggg cttcaccttc
     3841 acgcacggtg accagacgct ctttcggcgt catgtaaacg ctaaccggct ggttcaggtc
     3901 ggtaacaaaa cgcacgtcac gaccggtgat aatacccacc agttcgtttt cttcggtaac
     3961 gaccggatag cccgcaaaac cgttacgctc agtcagttct ttcacttcgc gcagcgtcgt
     4021 ggttggcaga acagtctgcg gatcagtcac cacaccagat tcgtgttttt tcacacggcg
     4081 aacttcttct gcctggcgtt caatggacat gtttttgtgg ataaagccga taccgccttc
     4141 ctgagccaga gcaatagcca ggcgcgcttc cgttacggta tccattgctg cggaaagcat
     4201 agggatattc agacgaatag ttttcgtcag ctgggtgctg aggtcagcag tattcggcag
     4261 aacggtagaa tgagcaggaa cgaggagaac gtcgtcaaac gtcagagctt ctttagcgat
     4321 acgtagcatg ggcaatatct cgaccagagt ggttaataaa tattgccgcg gcattataca
     4381 gagcgtaacc gattgcatct accccttttt gcaaaaaatg cttgctatcc ccgaagggcg
     4441 ggttactatc gactgaataa cctgctgatt tagaatttga tctcgctcac atgttacctt
     4501 ctcaatcccc tgcaattttt accgttagtc gcctgaatca aacggttcgt ctgctgcttg
     4561 agcatgagat gggacaggtt tggatcagcg gcgaaatctc taatttcaca caaccggctt
     4621 ccggtcactg gtactttaca ctcaaagacg acaccgccca ggtacgctgc gcgatgttcc
     4681 gcaacagcaa ccgccgggtg accttccgtc cacagcatgg acaacaagtt ttagttcgcg
     4741 ccaatattac gctctacgag ccgcgcggtg actaccagat aatcgttgag agtatgcagc
     4801 cggccggtga agggctgctg caacagaagt acgaacagct caaagcgaag ttgcaggctg
     4861 aaggtttgtt cgatcagcaa tacaaaaaac cacttccctc ccctgcgcat tgcgttggtg
     4921 tgatcacctc aaaaaccggt gctgcgctac atgatatttt gcatgtgtta aaacgtcgcg
     4981 atccttctct accggtgatc atctacccca ccgccgttca gggcgatgac gcaccggggc
     5041 aaattgttcg cgccattgaa ctggcgaatc agcgcaatga gtgcgacgtg ttgatcgttg
     5101 ggcgcggcgg cggttcgctg gaagatttat ggagttttaa cgacgaacgc gtagcgcggg
     5161 cgatttttgc cagccgcatt ccggtcgtca gcgccgtcgg gcatgagacg gatgtgacca
     5221 ttgccgattt tgttgccgat ctgcgtgcgc caacgccgtc tgccgccgct gaagtagtga
     5281 gccgtaatca gcaagagtta ctgcgccagg tgcaatcgac ccgtcaacgg ctggagatgg
     5341 cgatggatta ttatctcgcc aaccgcacgc gtcgctttac gcagatccat caccgattac
     5401 agcaacagca tccgcagctc cggctggcac gccagcaaac catgcttgag cgcctgcaaa
     5461 agcggatgag ctttgcgctg gaaaatcaac ttaagcgtac cgggcaacag cagcagcggt
     5521 taacacagcg gctgaatcag caaaatccac agccgaagat tcatcgcgcg caaacgcgca
     5581 ttcagcaact ggaatatcgt ttagcagaaa ccctgcgcgt acagcttagc gccacgcgtg
     5641 aacgtttcgg taatgcagta acgcacctcg aagccgtaag cccactgtca acgctggcgc
     5701 gtggatacag cgttactact gctactgacg gcaatgtact gaaaaaagtg aagcaagtta
     5761 aagcgggtga aatgctaacc acacgtctgg aagacggctg gatagaaagt gaagttaaaa
     5821 acatccagcc agtaaaaaaa tcgcgtaaaa aggtgcatta agccagcacg aattcaacgc
     5881 gttttttcga tatcaagcca tgcccgtgct gacagaagta atctactgca ccgcaggctt
     5941 ttaacacctg caacggttga tggcagtctg gacaaagagc tttcatttca ataatttccc
     6001 cgcaggatgg gcaacgcgca tggtcgttat cctgatcaag cacatgctga cattgtggac
     6061 aatgcagttc catatgaatt cctccggtag ccatccctct acattcatag agggaatggc
     6121 agataaaata cttacggata attatttatt tttcttgatg tgcttcatca gacgcttacg
     6181 tttacgcatc tgggttggcg tcagggtgtt acgcttgttc gcatacgggt tttccccttc
     6241 tttgaactga atacgaatcg gcgatcccat tacgtccagc gatttgcgga agtagttcat
     6301 caagtagcgc ttgtaggaat caggcaggtc tttcacctga ttaccgtgaa tcaccacaat
     6361 cggcgggtta taaccaccag cgtgggcata tttcagcttc acacgacgac cgcgtaccag
     6421 cggcggttgg tgatcttcaa cagccatcgt catgatgcgc gtcagcatag aggtccccac
     6481 acgacgggtg gagctgtcat acgcttcacg tactgattca aacaagttac caacaccact
     6541 gccgtgcaag gcagagataa agtgcacacg agcaaaatcg ataaagccca gacggaagtc
     6601 cagcgtttct ttcacctgct ctttcacttc ctggctcagg ccatcccact tattcaccac
     6661 aatgacaagt gagcgcccac tattgagaat aaagcccagc agcgagagat cctgatcgga
     6721 aataccttcg cgcgcatcaa tcactaacat caccacgttg gcatcttcaa tggcctgcaa
     6781 cgttttgatt acggagaatt tctctacagc atcggtgatt ttgccgcgtt tacgtacgcc
     6841 agcggtgtca atgagcacat actcacgtcc atcgcgttcc attggaatgt agatactgtc
     6901 acgcgtcgtg ccaggcatgt cataaacaac aacgcgctct tcaccaagaa tacggttagt
     6961 gagtgtagac ttacctacgt tcggacgacc cacaatcgcc agtttgatcg gcagactttg
     7021 cgggtcgaag tcgtcttcct cttcttcttc gccgttctct tccgcttcaa attgcgccca
     7081 gtattcagcg tcttcgtcga cttcctcttg cggtgcgaga tcttccatcc acggcagcag
     7141 cacatgctcc agcagactta atacgccacg accgtgagac gcggcgatcg ggtagatttc
     7201 acctaaacca agcgagtaga aatcaaccac tgcctgatcg ggatccagac cgtcagtttt
     7261 gtttgccacc aggaaggtcg gtttttcacg ggagcgcaga tgtttggcaa tcgcttcatc
     7321 tgccggcatc aggcccgcgc gcgcatccac cataaacagt acgacgtccg cttcttcaat
     7381 cgccagcagc gactgttccg ccatgcgggt ttctacaccg tcttctgtgc catcaatccc
     7441 gccggtatca atacagataa actcacggcc ttcaatttcc gcacgaccgt acttacggtc
     7501 acgagtcaga cccgggaaat ccgcaaccag cgcatctcgg gtgcgagtta gacggttaaa
     7561 taacgtggat tttcctacgt tagggcgccc gacaagcgcg accacaggta ccatgtttaa
     7621 agcctcattt ttataaatca tcagacaacg cacgctatat tcgcgtcgtt gttaaaaaca
     7681 ggaaaacggc ccctgtccag gagccgtttt caaagtgaac gacagagacg attaacgtgt
     7741 aatagagtac acggttccgt cttttgcctg gatcagcagt ttgccgtcag cggcaaccgg
     7801 ttcagtctgg aaaccggaac tatcaacttt ttgctgggca acgaaacgac catcttcgac
     7861 gttaatccag tgcagataac cttcactgtc accgaccacc aggttgccat tatacagcac
     7921 cggagaagtc agcaggcgat gcagcagatc gctttgtgcc cacagcgtaa cgccgccatc
     7981 aatggtcaac gccatcaccc ggtcattttg atcgaccaga tagatgcgat tgccgtcgac
     8041 gatgaaatca ttcaccgaac ccagttcgcg tttccacata atctgaccac tgcgcagatc
     8101 aagcgccgtc aggttaccat tataggccag cgcgaaaaca acgccgttaa caacgacggg
     8161 agtcgtgtca acatcgctca gacggtcaat ttcggtagaa ccggtcgcct gggaaatacg
     8221 ctgctgccaa atcatctggc cctgttccat cagcactgcg ctgacgcgac cattatcgcc
     8281 ccccacgacg gccgcaccaa aagccgttgc cggcgcagac tcgccacgca aagagagcga
     8341 aggcatatcg aggttaactg tccatttgac agcgccgtca gcttcgttca acgcttgtaa
     8401 ctgaccgtta ctggtgtgga ttaacaccag accgtcgctg accaccgggc gcgaaagtgc
     8461 ttcacccgcg actttagttt gccatgccac agtaccatcg ctggtattca gcgcgtaaac
     8521 ctgcgccttt tcgctgccaa tgtagacatg cccaccagac acggtcacac cgccagaaag
     8581 taatgcaggc tctttagaga accagccatc tttctcggcc aagctgacag accagatttc
     8641 tttgccatca tccgcattca gcgcttttac caaaccagcg cggtccgctg catagacaac
     8701 gttgtccgcc agtgccggat gaagattgga atagaagttg ccaatgccgc taccaacgga
     8761 agtactccac gccgtggtcg gcgtaaactg gttttcaacg gttggcaatg gggacatctt
     8821 taccacatct tcttcgctgt taaacagcga acagccgctt aaaagggtaa cggaaagcag
     8881 tcctggcagc agtaatttac gcaattgcat cgggtccctc tcagatggac aaattattaa
     8941 ttttcatctg catcatttcg ctcagtgccg gagtaacatc gcttttcacg cctgcttccc
     9001 atgcactacg cgcaccttgc ttatcacctt tgctcagcaa tgcttcacca cgcaggtcgg
     9061 caacaatggc agcccaccct tcacctttga tggtatcaag ggttttcagc gcggcatcag
     9121 cctgcttgag ctgtacctga acgcgagcaa gacgcagatt tatcacggct ttgagatttt
     9181 catcgctcgt gtctgccagc ccctgttgta actgggcggc agctttctct agttcatttt
     9241 tgtcaacaaa ttgctgcgca agttccaaag aagccagcgc accataagta tttttatttt
     9301 cagcagcaaa tttttccgcc gccgggatgc tatccggttt gccttcgctc actgcggtaa
     9361 ccgcattttg ataggcaaga gaagcggagc gtgcagaatc aacctgatgg ctgttccagt
     9421 agcgccagcc aatcagtgcg ccaacgccca aaatcacccc aacagccagt gctttgccat
     9481 tttcagcaaa aaagcgttta accgcttcta cctggtcgtt ttcgttctcg taaatttcca
     9541 cgctgtcctt ctccttcctt aacccagtaa cgtgcgcaaa tgcgcggcta cgctatcctg
     9601 cgcaactgcc gtttgctcac cagagcgcaa atccttcact actgctgtgc cgttagccac
     9661 ttcagactca cccagcacca cagcaacgcg ggcaccccat ttatcagcac gggcaaactg
     9721 tttcttaaag ttgccgccgc cgtggttggt catcaatttc acgcccggta attcatcacg
     9781 cagacgctca gctaatgcca tagccgcaga ttgtgtatca gcacctgaag ccaccaggta
     9841 tatatcgaca acaggatcgg ctttaaattc cggattaacg gcctgtacta acaatacaag
     9901 acgttcgagg cccatcgcaa aaccgacagc cggtgttgca cgaccgccca gttgttccac
     9961 aagaccgtca taacgaccgc ctgcacacac ggtgccctgg gagccgagac tgttagtcac
    10021 ccactcgaaa acggtacggt tatagtaatc cagaccacgc accagacgct ggtttacggt
    10081 gtaagcgatc cccgcgctct caagcagttt gcacagaccg gcaaaatgct cacgagattc
    10141 ctcgtccaga taatcaccta atgccggagc gtcgttgaga agcgcctgca cttccggatt
    10201 ttttgaatcc agcacgcgca gcgggttagt gtacatgcgg cgtttgcagt cttcgtccag
    10261 cttttcttta tgctgctcaa ggaatgccac cagcgcatcg cggtaattgg cgcgtgcttc
    10321 cagcgaaccg atagaattca gctcaagagt tacgtgttcg gaaataccca gcgcgcgcca
    10381 ccagcgggcg gtgagcataa tcagttcagc gtcgatatcc ggaccttgca gaccgaaaac
    10441 ttcgcagccc aactgatgga actgacgata acgccctttc tgcggacgct cgtgacggaa
    10501 catcggcccg atataccaca gacgctgttc ctgattgtac agaagaccat gctcgatgcc
    10561 ggcgcgtaca cagcccgccg tcccttcagg gcgcagagtc aggctgtcgc cattgcgatc
    10621 ctcaaaggtg tacatctctt tttcaaccac gtcggtgact tcaccaatcg cacgtttgaa
    10681 tagcggggtc tgctctacaa tcggcaagcg gatttcactg taaccgtagc tgccgagcac
    10741 gtttttcagt gtgccttcaa tgcgctgcca gatggccgtt tcgccaggca ggtaatcgtt
    10801 catgccgcga atggcttgaa tgttttttgc cacgtttatt ctctttctga atataaaaat
    10861 gaaccctcaa cgcttccctc aatgtttcgg gagctatgcg ggttcaatca tacacgggaa
    10921 gcgaggcgct tcccatcacg ttattatttt tcaacctgct gaacgtcaat tcgacgcgct
    10981 tcgtccagct gactggcttt cgcacgaatg cgtgcttcca gctggtcgat catatcgttg
    11041 ttgtccagac ggtctttgcg cacgccatct tcatagaggc cgcttttctt gttgccgccg
    11101 gtgacgccga gtgtagaaac cagcgcctca cctgggccat tcaccacgca gccgataatc
    11161 gaaacgtcca tcggagtgat gatatcttcc aggcgttgct ccagcgcgtt aaccgtaccg
    11221 ataacatcaa attcctgacg cgaacaggtc gggcaggcga tgaagttgat cccgcgcgaa
    11281 cggatacgca gcgatttcaa aatatcgaaa ccgactttga tctcttcgac cggatcggcc
    11341 gccagtgata cgcgcagcgt gtcgccgatg ccttcagaca gcagcagacc taaaccaatg
    11401 gcggatttta ctgccccgct gcgtgcacca ccggcttcgg tgatccccag atgcagcggc
    11461 tgatcgatct gttttgccag caaacgataa gactcaacag cgaggaagac gtcagacgct
    11521 ttcacgctga ctttgaactg atcgaagttc aggcgatcga gatgatcaac atggcgcatg
    11581 gcggattcca gcaacgcctg cggcgtcggt tcgccatact tttcttgcag atctttttcc
    11641 agcgatccgg cgttaacgcc aatacggatc ggaatgtttt tatcgcgcgc acagtcaacc
    11701 accatgcgaa tacgctcttc attaccgata ttgccagggt taatacgcag acaatcgacg
    11761 ccgtattccg ctactttcag tgcaatgcga tagtcgaagt ggatgtcagc caccagcggc
    11821 acgttaacct gctgtttgat gagtttgaac gcttctgccg cgtccatcgt cggtacggag
    11881 acacggacga tatcagcgcc aacgcgttcc agcgccttga tttgattgac cgttgcttcg
    11941 acgtctgtcg tacgcgtatt ggtcatggac tgtacggcga tgggagcacc atcgccaatc
    12001 ggcacattcc caacgtaaat acgtgttgat tttctacgtt gaattggagc ctggttatgc
    12061 atgaaaaatc tcccgcgtta cccgtctgtt actgcgccgg tgattgttcg gcattgaggg
    12121 tcagacgcgc aacctggtta gttctgataa aacgactcag atcgacaggt ttcccttgat
    12181 actggatctg tactgcggct ggcgcaccaa ttttcagttt gtatggtgcc tggccggtta
    12241 agttcaaatt accgtcttta cgctgcatac cgctaaacaa ttttttaccg gtagcatcag
    12301 taacctccag ccagcaatcg gcagtaaagt tcatcaccag cgcattcgga tcagccgccg
    12361 gcgtagtcac gcctgcctga tcggttggca acggcgcagc gccatctggc gttgttgttg
    12421 ccgttggtgc cggggtcgcg gcggtatcaa catttgcctg cgaaggcgaa acaaccgcat
    12481 tctgttgcgg atcaacagct ggtgctggcg cagttacggc aggtgtttgc gtgttggttg
    12541 cggtagtatc cacagacgct ggcggcgtgg acgttgtagc cgggtctgta gttgtcgacg
    12601 tatttaacgg aacactctgc ccctgctcgc tattactgct cagttccgcc gaagattgat
    12661 cggccatagt ggtgatctct tcctgctgag ctttgtggtc ttgccaccac caggcaccgc
    12721 tcaggccgat aaccacaaac aacaccagcc aggtgaaggt catcagccag ccgtcacgtt
    12781 ttttgcggcg tttaccgagg gaaaaactct gcataggcgc aacttttgca gcccgtagtg
    12841 gagcctgctt ttccagccct ggcagcagtt cttcttctgg aatatgtacc agacgcgcat
    12901 aagagcggat atatccgcgc aggaatgttg aagcaagatc ggcgggtgcc ttatcttctt
    12961 caatgtcgcg taccgtggaa accttcaggc aaagtcgctc ggcaacggcc tgctgactaa
    13021 gtcctagttg ttcgcgagca ttacgcaggc gagcgccggt agtaagtgct tcattttggt
    13081 cgtgcgtggc ttcagtattc attcgctgca ggtacgttta aatgagaatt gagatgccgg
    13141 tgaaccatca tgctcaccca caccgcgaaa catccgttaa gttaaccgtt atcatacagt
    13201 ataagaccgt ctgtgtgctc gtgacaaagc ccacattcac taaggctaac ttactgttgc
    13261 atcgttacat actgccttaa agtcagcaaa aacgcaccgt taatattgac cagacaattg
    13321 caacttaata cttcattcat aaaagattac gccacggtac ataaagtaac cgtggcgtaa
    13381 tggctatcag accgctttaa tgtcgatggc ttcaccctgc atccgtttac gcagggtacg
    13441 tttcgtacgg tcgataacat cgcccgccaa ctgaccacag gcagcatcga tatcatcacc
    13501 acgggtttta cgcacaatag tggtgaaacc gtagctcatc agcacttttg agaaacggtc
    13561 gatacggctg ttcgagctgc gtccatacgg cgcgcccggg aaggggttcc acgggatcag
    13621 gttgatctta cacggcgtat ctttcagcag ttccgccagt tggtgcgcgt gttcagtgcc
    13681 gtcgttaacg tggtcaagca tcacatactc aatagtgaca cggccctgat tggcgttgga
    13741 tttctccaga taacggcgca ccgcagcaag gaacgtttcg atattgtact ttttgttgat
    13801 cggcacaatt tcgtcacgaa tttcgtcgtt cggcgcgtgc agggaaattg ccagtgcaac
    13861 gtcgatcata tcgcccagtt tatccagcgc cggaactaca ccggaagtag aaagcgtgac
    13921 gcgacgttta gacaggccaa aaccgaaatc atcaagcatg atttccatcg ccggaacgac
    13981 gttgttcagg ttgagcagcg gctcgcccat gcccatcatc actacgttag tgattgggcg
    14041 ctgaccggtg acttttgctg cgccgacgat tttcgccgca cgccacacct ggccaataat
    14101 ttccgatacc cgcaggttgc ggttaaagcc ctgctgggcg gtggaacaga atttacactc
    14161 cagcgcacac cccacctgcg aagagacgca gagcgtggca cggtcgtctt ccgggatata
    14221 caccgtttcg acgcgctgat cgccaacggc gatcgcccat ttaatggtgc cgtcggatga
    14281 acgctgttct tcaaccactt ccggtgcgcg gatttccgcc acctctttca gtttgccgcg
    14341 caacactttg ttgatgtcgg tcatctcatc aaagttgtcg cagcaatagt gatacatcca
    14401 cttcatcacc tgatcggcgc ggaagggttt ttcacctaaa tctttaaaaa actcccgcat
    14461 ctgctgacgg ttgagatcca gcaggttgat ttttccatct ttcgtggtga cgttttcagg
    14521 tgtgactaat tgttcagaca tatgctattc cggcctcgtt attacacgtt atggcccctg
    14581 gaggattgaa aaaagaaacg ccccggtgag cggctgctcg tccgggggcg ctgcattgta
    14641 caaattctgg cgcacggatg ccacgtttgc acgcggcatt tacgaaatta ttaacgggtg
    14701 cgcgggcaca cttcgccttc gccaaagaaa taagcgattt cgcgagcggc agattcgacg
    14761 gaatcagaac cgtgggtacc gttttcggtc aggctgtcag cgtaatcagc gcgcagagta
    14821 ccagccagtg cgtttgccgg attggtcgcg cccagcagat cgcggtgacg ctgaacggcg
    14881 ttttcacctt ccagcacgga aaccacgatc gggccagagg tcatgaattc aaccagacca
    14941 tcaaagaacg gttttccatc gtgttcagca taaaagccac gtgcctgttc aacggtcagg
    15001 tgcagcattt tggtgccgac aattttgaac cctgcagctt caaagcgcgc aaagatatta
    15061 ccaatgacgt tttttgctac cgcgttcggt ttgatgatgg aaaaagtacg ttcaatagcc
    15121 atttttacct ctgtaaattg ttctgttgtt gtctgtacca gcgtacgaga tggcgcggat
    15181 tataatgagc aacagggccg ttgactattg atgaaggtaa cattttttta aaataaaatg
    15241 agttttagca acaatcaggg gcaaaacaga ctattgcatg acaaatttca ctgtcgcgat
    15301 ttgccccaca tcatccatca ccagcaattg ataatcacct ttatccgtca aatgcagggt
    15361 cacgttgcgc ccgcgttcag ttaacggttc gccattcaga aaccaccagc gttcacctgc
    15421 ccctccactt gattgcaacg gcaaagtcgc ttccgctgcg cccggtaaac gtttaataat
    15481 cgcgccatcg cgaacgcctg tcagctgcag agggagcttg gcatcgtgac cgtatggcgg
    15541 gcaaattgtc gaggccggtg gtaagcgcac agcgcgacgt tctgatgcgg gcagccaggg
    15601 ttccagcggt agcggccaga catttatcat ttcttgtcgc gcttgcgggc aatcagcggc
    15661 aacacgtttg ccattttcat ccagccagat ggggaaacga atgccattaa tgccttcctg
    15721 ctccggcagt aatagagttg gcggctgact cccgtccagc agccaggttg ccaggcggcg
    15781 gcgacagtta ccgtcacctt ccggcaaaga ctgtccgccc ggccagcaga taacgccacg
    15841 agtgactgag ttcgggcgcg ggtcttccgg cagattcgca ctgcgcgaca gtaagatatt
    15901 attgacctga ttcaacaatg gtacggcact ggcaaagcca aactgaccaa caacgggcgt
    15961 gccgtccggt ctgccagtcc agatcccaat gacatagcga gcgttaaccc caatcgccca
    16021 ggcgtcacga tagccatagc tggtgcccgt tttccatgcc agtggggcga cgcgcggcaa
    16081 ggcactatcc ggcaagggtt gcgcttcatc agccataatc cggcgaatga tccacgccgc
    16141 ccccgacgac attaaaggcc gttcaagcag cggatcgtca ggctgtaagc gcaatttgcc
    16201 tgccttgccg tggcgagcaa acgcggtata cgctgccgcc atatcttcca gttttgcacc
    16261 agcgccgccg agaatgagtg aaagattcgg cgcagcaccg ttgggcaaat ataacggcaa
    16321 tccaacattg cgtaactttg ccgcaaaccg tttcggtcca taggcttcca gcacctgcac
    16381 agcaggtaag ttcagcgagc gcaccagcgc ctcgctcatg ctgatcgggc catgaaaacc
    16441 gctatcaaag ttacctggtc gataatcacc ggtgcgccgg gggacgtctt gcagcagtga
    16501 tgccgggtgg atcaagcctt catccagcgc cagaccataa acaaacggtt tgagcaccga
    16561 tcctggcgat cggatcgcat tgaccatatc aacatgacca aagcgtgaat catcgttgag
    16621 atcaaccgat cccacccagc cgcgaacacg catatcggta tgatcaacca cgatcatcgc
    16681 cagtgagctg cgcggtggca accgcccttt ccagttttgc gccagttctt ccagacgtcg
    16741 ttgaagaccg gcatccagcg tagtaacgat tttgtcgctt ttgcttttac cgagcatcat
    16801 gcgcgaaaac agcggtgcca gttgcggcat ttgtcggggt gccagccaga tgggttcttc
    16861 ccttgactct tttacctgct cacgggacca cacaccttgc acggccatcc gttcgagcac
    16921 tttattacgc gcggcttcgg cacgctccgg ccaacgatcc gggcgaagac ggctgggcgc
    16981 ttgcggcaaa accgccagca ttgccgcctc ggaatagctt aaattcgcag gcgactttcc
    17041 gagataagcc caacttgccg caccgatccc ctgcaacgta ccgccaaacg gagcgcggtt
    17101 aagatacaag gtcagaattt cacgcttaga cagatgccat tccagttgca acgcgcgcca
    17161 gagctggcga attttgccgc caaatgtttt ggggtgagga tcaagcagac gagcaacctg
    17221 catagtgagc gtgctgccac cggaaataac ccgtcccgaa gtgagatctt gccatgctgc
    17281 gcgcgccacc gagaatggat tcacccccgg atgcttccag aaccagcgat cttcataatt
    17341 gatcagcgct tcaaggtaac gtggagaaac atcttcgatt gttaccggat aacgccagat
    17401 gccgtcagca tcggcgaagc gccatagcgg cgtaccatcc tgcgccacga ccactcgtgc
    17461 gggattgact tcatgcagcg gtagaggcca gagtttatct gctccccacg ctgcaagaaa
    17521 aaggagaaat ggcgcagctg ccagcgttat ccagtagccg cgtttggtta acaagcgagg
    17581 catttacggt cggacaatca gcagatcttc agccgttccg gtcgcccgcc attggggaac
    17641 atacattgat tccaccatcg gttgcggtac ctgatacgtt cccggcgtca ccgcccgcgc
    17701 cagatacacc agcgttaccg gttggtattc atcaacggca accgccgcca caaagcgatc
    17761 gtcacggaac tcaatgtgct taatactcgc ctgctgcatc tggttcagta agttttgcac
    17821 ttcgccacca ctttgctcca ggctggcgct accgttcgcc agattctgat tttccagttc
    17881 cagacccgca ggcagcagat ccacgactaa cgcatccggc acgctgttac tggcttttac
    17941 ctgcaaccag accagcacca gatcgccgct acgtaacgag tccagcgatt tgctcttacc
    18001 atcagtacca agaatatgac gctcgatttg cagcacattg ttcgccggta aaggtgcgga
    18061 ttgcggataa ccgctggcat ccatacgcaa ccataacggc tgatcgccac tgtttctcac
    18121 ctgcaaggtg gcaagttgat cgctattcag attgctgttt agcgttttct cgcctgtcag
    18181 cggctcagct gagaaagagg tttgcgcctg ccatttaccc ggtaaatcct gaatcgtacg
    18241 ggcagccagg aacaacgcgt tactttcctg cgtcgatagc cagcgttcac caaacgcctg
    18301 ctgcgaaagt gtgttcagca aagtgtactg ctcatcgggt agtagtttat tttcttccag
    18361 caaggagagc attaacgcgt tgtcgcgcag tggactaccg taatcaccca gccatatccg
    18421 ctcatcacta tttctcggcg ttttcagcgc cagcacaatc gcttcttcac cacgcgttgc
    18481 atcacccatg gttttcagcg caacgccaag ttgcagcagc ggtaaaccag aagcggcatc
    18541 tgcacgatgc tcccagattt cacgcagcgc acccagtggt gctttttgct gacgggccag
    18601 caccagcgca gcgtaagact gaacggcgaa tttactggct ttgagattat ccgcgtacgg
    18661 gatcgacatc atgcccggat cttgtaaata gcgcagcaga cgctcattac cccggttaat
    18721 ggcgtctgtc ggcacgctgt aaccctgttc gcctgcgcgg accaggaaat ccatcacgta
    18781 agccgtcagc cagtactctt cgtcaccgtt tttatcccac agcgcaaagc cgccgttatc
    18841 acgttgcatt tgcagcaaac gggaaatgcc gatatcgacc gatgcacggc gtttctcatc
    18901 actgtcgcct ttgatgccca acgcctgcag ttgggctgcg ttggtataaa gtgacgggaa
    18961 caggccgctg gcggtttgct caagacagcc gtacggatac gcttttaact ctttgatata
    19021 acgtgcaatg ttcagcggtg gtttgccgct caacaacaat tgcccttcca gcgtaacagg
    19081 cgagaagttt tgcaatccat ccgccggaat cgcccatgtc tcaccaggct gtaacgccgt
    19141 accgtaatta accgtttgtg ccgggaacgc cggacggacg ccgattttcc actgcttatg
    19201 ctgatcggca acggtttcac ccggtaacgc taacccgcta atggtggcct gaatttctcc
    19261 atcgccataa cccggcaatg ctcgcaccgg gataaacagc gtagtacgca cacctggcgc
    19321 taattcaacg gctgcgggtg aatcgctgac cagttcaagc aaaccactgg cggtcagggc
    19381 aacgttcagt ttttgcggtt tatcggtaag attagtgata tccagcgtca gacgcgaggt
    19441 atcgccactc gccataaagc gcggcatgtt cagttcagca atcaccggtg cggcaactat
    19501 cactttgctt tcgttgctac cgaaatcatc tgccgtccag gcttgcgcca tgacgcgcaa
    19561 ttcaccgtta aaatcgccaa tcggcagtgt aaccgagcct tcgccctgtt cgttgagcgt
    19621 taccggcagc gcctgctgcg cgacaatatt gacgtgattg accggcggtt taccaccacg
    19681 tttcagctca tcaccatcgc caccgaaacg cagagctgcc agacgcccct gaccttcaat
    19741 aacctgaccg taaatatcgt aaatgtctgc gccatagcgt ttctgaccaa agaacgcctg
    19801 ccacggatct ggcgtgacgt agtcagtaat attcagcaca ccactatcaa cggcagacac
    19861 cagcacattc acctgtttag gcttctcgcc atttttagtg ctggctttaa ttttcacggt
    19921 taatggttga ttgggacgca tttttgctgg tgtttccagc gccagatcga ggcgacggtt
    19981 ttcatcgcca agcggcagat gcaacacacc aaccgcgcgt tttggcgtcg cggagcgaga
    20041 tttatcgcca ggacgtacca ccagcgtact taaatacaga tcatgacgat tccaggtttt
    20101 atcgaccgga atcgtcagat ccagcccttg agcccgaaca tcaatctctt gccaccacag
    20161 cggcccttca ctggactcga ccatcgcata acctttaccc gccgttggcg cggcgatatg
    20221 caacttaatg gtgtcgccag ggcgataact ggctttatcc agtttcagcg tgacacggtc
    20281 gggtcgcact gcgccgctac cgtcgctgtt gtcctgccag ctatagccag cccagaaacg
    20341 aacactactg accgcttcat tcggcgcttt gacttccaga cgataagcgc cccactctac
    20401 cggaaaactg accttgccgg tttcgtccgc tttcagatcc agagtttgtt cattttcgat
    20461 cagatctttt tgatcaaact gtgactgcca gccttcatct tctgaccagt tccagtagta
    20521 atcgcggcgt tcgcgaatca ggcgcacctg caagcccgac acggcttttt tcacgccttg
    20581 cgcatcgcta taaacgatgt caaaagcagc gttactgcct tcatcaacaa tgggctgttt
    20641 caccgtgctg tcagtacgat aatcgtaaac cgatttcgag gcgaactgcg gacggatccc
    20701 cggcaatgca tcggcaggcc agatagcctg ctcagcgcgg cgcgtcaccg ggcgaccgcc
    20761 cgattccagc aaactaccct ggaaaataac ctgtaatggg gaatgcgttt ccttccactg
    20821 gctttctgta gaaacttcgc cgcgcccttt atcatccagc gtcaactgaa cttcatccag
    20881 cgtgcgggaa agattttcgg cagctatatc gccgaattca aaaccaggta aggctgacac
    20941 agcttcacgc agtgggcgca ggaaaagttg cccttgcaaa gtattaccat tagcaggtgc
    21001 accatacagg tagtacccca ccacggagaa tttcacttca tctttcggcg ttagcggggt
    21061 tttctcaccg gtcagattca gcgccatgcg ctctggcata aaatcttcga cgtggaaatc
    21121 ccacatccga tactggttat cgcccgtgtt agcgcgaata tgccacatac cggttgccgc
    21181 attgctatcg agtggccagg taaagtggta gaggccattc tccggctgac taacgacgct
    21241 cctgagtacc tgcccatcgg gtttaatcac gtctaacttg atgggttgat tgggcaacgc
    21301 tttaccgtct gcatcacgca gcaaaccatt gaggattacc gtttcacctg ggcgataaag
    21361 atcgcgtggg ccaaacatga aaaactgttt gctatagcct ggcgcgccag caatgttaaa
    21421 ttctgctaag tccagcgccg gaagttttaa atcgagtagc gttgtctgac cgtctttacg
    21481 cgccaacagt aatgccgcgt ttttatcatt ttccagctgc acatgcccct gagcgtcact
    21541 ggttgcctga gtcagagtct gccctttctc atttaataaa gagacttcaa ttccttgctg
    21601 ggccgcgccg ttttccagac tttgggtaaa gatatccaga cgattgtgat aacggtgagc
    21661 tgaaacgccg atatcactta acgtaaacag cgtcgcggga ttactgtaat cgtaacgtcc
    21721 agcctgattc atcacagcca gatacacgcc cgcctgctga agcggtttga tatcgcccag
    21781 cggcagcaat aatttttcac gggtgttacg cgcaggattg agatcaaacc gtccggtgta
    21841 gaccagatcc gccatctgca gcagtttgtc tgactgccag ttcgccagcg aattgcggta
    21901 ttcccattgg ctaatgaatg ctggcagaga ttctggctta acgcggaaga agttaacatc
    21961 gacattatta acgttgagcg ccattaccgg cagcccttca acgactttgc caggcagcag
    22021 cgaaccacgg ctggcaaaac cgacgctggg ttggatgtcg cgggtagtta tagttttttc
    22081 gtaatcttta ctgaaggttg cgttgttgag tgctttgacc tccttgccaa tagtaacgat
    22141 caaatcacgt ttcggttcga ggtggcgtaa acgtaactct ttaagattat ctgacagctc
    22201 ccaggcacca tccactttgc cgcttttttt atcgacgaca tgaataacgc gtgagaaatc
    22261 ctgatccggg tcgagaggga tggagaacgt cagcaccagc gtggctgcac catcaagttg
    22321 gacttcagag agatccagca atgtcagcac cttaccggca ctctgttggg ccagtttttg
    22381 tctctccggc acggagagtt ttgctgaact cgcgttttct gaagaggccg ctttagtaac
    22441 ttcagaaggc gcatcttttt tcaccgctgt tggcgcgtta tcgttgttgt cgcaccctgc
    22501 cagcgccagc attagcatgc aggcggctac gcgtaacttt ttcatttttc atccctgcca
    22561 caatggcccg ttagcaacgt cgaataatta ttatgcgtga gaatttacgt tatgtaattc
    22621 agtatcacgc ccatacaatc cgaattttag tgagaattgt ggctcagaca gccaaatcag
    22681 cctctttcgc ttgtcgcttt tcactaaaca tccgacaatt tagcctaacc ccggcaaaaa
    22741 tggagatgcc tatgtccacg acatggtttg taggagccga ctggctcgcc gaacatattg
    22801 atgacccgga aattcagatt attgatgccc gcatggcgtc gcctggacag gaagatcgta
    22861 acgttgctca ggagtatctg aatggacata ttcccggcgc agtgtttttt gatatcgaag
    22921 cgctttctga tcacacttcc ccgcttccgc acatgctgcc gcgcccggaa acgttcgccg
    22981 tggcgatgcg tgaattaggc gttaaccagg ataagcacct gattgtctat gacgaaggta
    23041 atcttttctc agccccacga gcatggtgga tgctgcgcac ctttggtgta gagaaagtgt
    23101 cgattctggg gggtggactt gcaggctggc agcgcgatga tctgctgtta gaagaaggtg
    23161 cagtagagct gccggaagga gagtttaacg ccgcgtttaa tcctgaagcc gtggtgaaag
    23221 taaccgatgt attattggca agccatgaaa atacggcgca aattattgat gcccgcccgg
    23281 ctgcacgttt taacgcagaa gttgatgaac ctcgcccagg tttacgtcgc ggacatattc
    23341 ccggagcact gaatgttccg tggacggaac tggtgcgcga aggcgaacta aaaacgaccg
    23401 atgaactgga tgcgatattt tttggtcgcg gcgtcagcta cgacaaacca attatcgtca
    23461 gctgcggctc tggtgtaacg gcagccgtgg ttttgttagc actcgcgacg ctggatgtgc
    23521 caaacgtgaa actgtacgac ggcgcatgga gtgaatgggg cgcgcgggca gatttaccgg
    23581 ttgagccagt gaaataagta ttttacaggc aataaaaaac cgccgaattt ggcggttttt
    23641 tattgctggt ctggttcgcg gcctttccag caggttgtat taccgtagta atgcaagcgc
    23701 gtctcagcgg agacaatact cgccagtaac tctctttttg tcaagcaaaa gagagttatt
    23761 attgttctgt tagtgtatta tccactgcgg ccctttccgc cgtctcgcaa gcgggcgctg
    23821 gcttaaggaa aggatgttcc gcgaccgtta gagcaagcgc gtctcagcgc ttgctatcgc
    23881 ggcaatatcg ccagtggtgc tgtcgtgatg cggtcttcgc atggaccgca caatgaagat
    23941 acggtgcttc tgtatcgtac ttattgtttc tggtgcgctg ttttcccaag taaataataa
    24001 ccggagtctc tccggcgaca atttactggt ggttaacaac cttcagagca gcaagtaagc
    24061 ccgaatgccg ccctttgggg cggcatattt tagattatcc gattctgttt aaagtcacgc
    24121 aaaaaaccac cccagcgacg ttcatagaat ggcacaatat gttcggtaat aaagtggcta
    24181 attccttttt cccctttttt cacctgacaa atatcgattg gttcatcgcc aggtaatgta
    24241 tcggtcgcta cacttcccgt cgcctgaata atttcttcga tatcaccatc ggcttcaatg
    24301 ccaataagta aattaggctg tgcctcttcg ttctctttaa ttgaacaaat aaaagcacgc
    24361 ttcaccgact taatggtttt aaataaggtg gtgagtgaat caatcatttg tgctggcggc
    24421 tctgcgactt ccgataatat cagcgattca ccgccttcca ggatttcctg gctgctcagc
    24481 ggatttccct cttcaccaat caacaaactg atttcacgcg gcataaattc tttaccggtc
    24541 ggcagtttgg cattaaggaa gagcgtttcg ccaagggtca tctcaaacaa tgtgcgaacg
    24601 ggcattacga caaatgcctg ttcgtcttca accgcctgtt gaagcgcttc taacgaggtg
    24661 aaaaaaggaa tgacgctggt gccgtcttct ttttcccagt gctgtaaatc aagcgcacta
    24721 tcttcaacca cagcctcgcc ctgcgccgcc gtaccaggca cccagacggt ggattccagt
    24781 agagtacgga aaaaggccgg gcggtgcgcc ggttcggttg ctgctttttc cagcaggtct
    24841 tcaagttcgt tttttgtttc ggacataaga accacaattc attcaacgtt cggggcgcaa
    24901 atgcctgatg cgctacgctt atcaggccta caagggattc gcaatttgtt gaatttgcag
    24961 aatttgtagg ccggataagg cgtttacgcc gcatccggca tattagttta cgccgttaac
    25021 agattagcaa tcgtgcgcac accaagtccc gtagcgcccg cagaccactg ttcaaccggc
    25081 gctttacggt aagtcgccga gcagtcgata tgcagccagc cttgctgata gttctcaaca
    25141 aagtgcgaca ggaagcccgc cgccgtgctc gcgcctgccg gatacgccgc gcttccggta
    25201 ttgttcagtt cggcaaagtt agacggcagc tggctgcggt ggaattccgc cagcggcaga
    25261 cgccagaacg gttcgttctc ttgtgaggca ctcgccagca aacgaccggc aagcgcatcg
    25321 tcaaaactga acagcgcgtg ataatcatta cccagcgcag ttttcgccgc cccggtgagg
    25381 gtcgccgcat caatgatcag ttccggtttc tgcgcactgg catcaatcag accatcggcc
    25441 agcaccagac gcccttcggc atcagtgttc atcacttcaa cttttttacc gttgcgatag
    25501 gtgatgatat cgcccagctt gaacgcattg ccgctaatca ggttatccgc acagcagagg
    25561 aacagcttca cgcgcttgtt cagtccgcgc gtaatggcaa atgccagcgc cccggtaacc
    25621 gttgccgcgc cgcccatgtc cgacttcatc gagtccataa acgcagtctg ttttatgctg
    25681 tagccgccgg agtcaaaagt gataccttta cctaccaggc acgcgtacac cggtgcttct
    25741 ttatcgccag ttgggttgta atccagcgcc agcaataccg gagaacgttc tgaaccgcgc
    25801 ccgacagtgt gcagccccat ataaccttgc tcacgcagat cttcgccttt ggtgatccga
    25861 taagtcacac gatcgcccgc gacgttgctg atcagatcaa cagcacgctg tgccagttgc
    25921 gatggtccca attcttctgc cggtgcgttg atggtgtcac gcacccagtc gatgatcatc
    25981 aggcggttat ccagttcctg gcgctgggca tcgtccagat ccggccactc cactttacgc
    26041 gtgcctttcg gggctttgta accttgccag aatgcccagc agcgatccgc atcccagcct
    26101 tcaccgctta actgaacatg cttgatgccc agaccgtcaa tcttgcgcgc cgcacgctgg
    26161 atcagcccca gatcgtctgc cccgttcaaa tgcagggtaa tgccgtcatt attaatgctg
    26221 taagttgctt tttctcccca gcgcgcgtcg gcaggttggg tagagagggt aatcttcatc
    26281 gcttctgtca ttttagttat ccttctttgt aaaagggccg ccagttggca gccccaggat
    26341 ttttccgcta aatgattcgc gttgcaggaa ggcggcaagt gagtgaagcc ccaggagcat
    26401 agataactat gtgactgggg tgaacgagcg cagccaacgc atctgcggcg tgaagcatga
    26461 cgcggaaatt attcggcctc gtccagccag actaacaaaa tcgcttcgag gattttctcg
    26521 ttggatgcct gcgggtcgtc gtcgaaatct tccaaatcgc aaatccactg atgcatatcg
    26581 gtgaatcgaa ccgttttcgg atcaagatcg ggatacgcat cgtacagtgc ttcgccaatt
    26641 tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca cgcgcatggt
    26701 tgatagtgta acgcgggatt tcgaccacta aatcttcgtc ggtgacgcgc gcctggcagc
    26761 ttaaacggct ttccggctcc agtccccagg ctttgtccag catgtcgtct tcctgctctg
    26821 agctttccgg cagcgagtca aaaccttcac gaacgatgca gtggcaggtg gtgcaagcac
    26881 aggatttttc acaggcgtgt tcaatctcga taccgttacg cagagctgcg tcgagaatgg
    26941 tttcaccgct attagcttcc agaacagcgc catcagggca gagatcctga tgaggcaaaa
    27001 taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc acgacgaacc
    27061 gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt tttaatcgct
    27121 tgttcgatgg cgtcaacgtc atcgccctgc gccacttcac tcaggtgagc ggcagcatcg
    27181 tcaatgacct gacgttctgc ggcgcttaac agcgcggcat cagcagccag cgcgccgtgc
    27241 agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcattcg ggcttttacg
    27301 tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct atcggtcaga
    27361 ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc catcgccgtc
    27421 acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc accaccagcc
    27481 ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc ctgcaccagt
    27541 tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt aaaggtggtg
    27601 aaatcctgag cgcgggccac cggaatcgtg gtattacgcg gaatcacttt ctccaccagg
    27661 ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcagcag catttcgctg
    27721 tccggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc gacgacttta
    27781 tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg ttcacgcacc
    27841 agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcatc agcttctaca
    27901 cccgcgtctt tcagcgcgcg acgacaagcc agtaaggttc gttttaccag tggcgcgatc
    27961 agttgattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt aacggtcacg
    28021 gagtccgcat cgctcagcgc gattttggct gcaatggcgg catccagcag ttcacgctga
    28081 acgcggttat cgctacgatc aggaatgccc gcctgctcgc gaatgtaatc cgccagcaga
    28141 tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac ttcaaacacg
    28201 ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc ataaacagcg
    28261 atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcggt cggttcgtta
    28321 agtaagcgca gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc ctgacgctgg
    28381 gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc tgccagggct
    28441 tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac cgggttcagc
    28501 agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa ttgataaggc
    28561 agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg tttaacagaa
    28621 ctaatggtgt tggcggtatc aagcgctgcg ttagtacgcg cgtcataacc caccgaatgc
    28681 ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg gtcggctaac
    28741 gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc caggtcaata
    28801 ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact aatttgtaat
    28861 aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga gttgttcggc
    28921 actgctccgc agtttatcga gaaaacgcag cttacgcacg gtatccgccg ccgcgtccca
    28981 cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca tctttttcac
    29041 acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct cgtccagctc
    29101 ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat gctgctcgct
    29161 ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg gatgacgcag
    29221 cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt gttccgcctg
    29281 gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa aacgcaggct
    29341 cagcgcctgg gtatcgagtt gataacgggc aggcaagcca aagagggtga agtaatccat
    29401 aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac aaaccccacg
    29461 cgcaggcgac cacgatgggg ttatcggtat gcgcatcaaa cgtggaagct ttcgccgcaa
    29521 ccacactcat ctttgacgtt cgggttggtg aatttaaacc cttcgttcag gccttctttt
    29581 acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac cacgactttc
    29641 acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa ttccagtaca
    29701 taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa ccctttgccg
    29761 cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt aatcgacata
    29821 accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa tggcggcttt
    29881 gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt caagttcttc
    29941 agcaatatcg gtatttttga tcgcctgcgc ttcgtcgaga gacttccctt tcacccattc
    30001 ggtgaccagg gagctggaag cgatagcaga accgcagccg taagttttaa aacgcgcgtc
    30061 ttcaatgata ccttcatcgt tgactttaat ctgcaacttc atcacgtcgc cacaggccgg
    30121 tgcccccacc atgccgctgc cgacgttctc gtcgttgttg tcaaaggaac ccacgttacg
    30181 cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt ctcctgattc
    30241 cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct gcttgtacat
    30301 ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta actcgatggt
    30361 gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag agctatgtgc
    30421 cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc ttgctgacgt
    30481 acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg actcaccttc
    30541 aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt caccgttcag
    30601 gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc cgcgcagacg
    30661 ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcctcgccca ttccgacgat
    30721 ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc cgtgcatttg
    30781 cgcttcgatg cgtacgcgcg gtttacgacg tacatacagc gcaccgatac ctttcgggcc
    30841 atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca ggtcgatagg
    30901 cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag cacggcacat
    30961 ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt gcatgatgga
    31021 aacaaggatg gtgtcgtcac gcatcgctgc ttcaagttct ttcaggtcga taatgccgtt
    31081 acgctgcggt gccaggtagg tgacttcaaa accttcgcgc tccagctgac ggcaggtatc
    31141 cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt tctgataaaa
    31201 gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg taaagacgat
    31261 ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat ctaccgcttc
    31321 ttcagcctgc cagccgaaac ggtgagaacg agaggccggg ttaccaaagg ttccgtccat
    31381 cgtcataaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg ttgcggagta
    31441 gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc aatgcaagga
    31501 accaggctac cggctggatg tacgaccgtg tttacggagc gcgatacgcc ccggcctgat
    31561 tctgaattct ttttattaag cgcgtaactt aacgtcgatc gcgtcttgta tgcgggtgcg
    31621 tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt tattaaccag
    31681 ttcgcctaaa gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat cacgccacag
    31741 cgcgtgggtc aggcatttat cgccgccctg gcagccgcct ttaccctgac aacgggtggc
    31801 atctacagat tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc tggcatcttt
    31861 gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat ttttacgcag
    31921 acgggaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg aaatatcagc
    31981 caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg tcacggcata
    32041 gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc tgcccggggt
    32101 tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt cccgagtaaa
    32161 ttggtcaact atttacttga ctgatttagt cgggtattta accttcagtg ccattttttt
    32221 atcgtggcgt ttgtacctgt tgtccttcca gaccatgggc gggttgaggg caattattcg
    32281 cattagcgct ggcctcttcc cctcacccta accctctccc cgaaggggcg aggggaccgt
    32341 ccgtgcattt atctggctta gccctcaggc cttattacct ttattctgct gctcaataga
    32401 agccagaatc ccgcgcagga tattcaattc ctggctttcc gggcgcgcac gggtaaacag
    32461 acggcgcaat ttattcatca cctgccccgg atggttttca cggataaaac cggttgccag
    32521 cagcgtttgt tccagatgac cataaaaacg ctccagatca tcgaccagcg gatacggcgt
    32581 ctcttcatgc tcgacctgct cgccgttttc ctgagttgcc agccaggcca tgcgcacttc
    32641 ataggcaata acctgaaccg ccatcgccag gttcagcgag ctgtattccg ggttagccgc
    32701 aatcgcgaca tgataatggc atttctgcaa ctcttcattg gtcaagccga cgcgctcacg
    32761 accaaacacc agcgccactg gggtatttgc cgcttcagcg acgcttttca gaccgcattc
    32821 gcgcgggtcg agcatcggcc acggcagcgt gcgggagcgt gcgctggtgc ccaccaccag
    32881 gctacaaccg gctaacgctt cgtcgagcgt atcgacgatg tgagcattac caatcacatc
    32941 gctggcccct gctgccaggg caatcgcctg ggagtcgggt ttcaccagtg gattaaccag
    33001 ccacagattg gttaatccca ttgttttcat ggcacgggca acagaaccca tattgccggt
    33061 gtgtgacgtc tccaccagca caattcgaat attttgcagc attgtctttc ttcatctaaa
    33121 gattattcac gcatcttatc ataaaacgaa gacagatgcc gatctcgctg ctatactctg
    33181 cgccgttttc ccgttcttta acatccagtg agagagaccg atgcatccga tgctgaacat
    33241 cgccgtgcgc gcagcgcgca aggcgggtaa tttaattgcc aaaaactatg aaaccccgga
    33301 cgctgtagaa gcgagccaga aaggcagtaa cgatttcgtg accaacgtag ataaagctgc
    33361 cgaagcggtg attatcgaca cgattcgtaa atcttaccca cagcacacca tcatcaccga
    33421 agaaagcggt gaacttgaag gtactgatca ggatgttcaa tgggttatcg atccactgga
    33481 tggtactacc aactttatca aacgtctgcc gcacttcgcg gtatctatcg ccgttcgtat
    33541 caaaggccgc accgaagttg ctgtggtata cgatcctatg cgtaacgaac tgttcaccgc
    33601 cactcgcggt cagggcgcac agctgaacgg ctaccgtctg cgcggtagca ccgctcgcga
    33661 tctcgacggt accattctgg cgaccggctt cccgttcaaa gcaaaacagt acgccactac
    33721 ctacatcaac atcgtcggca aactgttcaa cgaatgtgca gacttccgtc gtaccggttc
    33781 tgcggcgctg gatctggctt acgtcgctgc gggtcgtgtt gacggtttct ttgaaatcgg
    33841 tctgcgtccg tgggatttcg cggcaggcga gctgctggtt cgtgaagcgg gcggcatcgt
    33901 cagcgacttc accggtggtc ataactatat gctgaccggt aacatcgttg ctggtaaccc
    33961 acgtgttgtg aaagccatgc tggcgaacat gcgtgacgag ttaagcgacg ctctgaagcg
    34021 ttaatgactc aggcgggtga tgtcactcac ccgccctcgc ctttcaggcg ctattctgaa
    34081 atacttcctc accgctttac tttctttcgt cactctccca ccatctttcc ccgattaatg
    34141 gatgataaag aagtcgcaag gaaaggttat ggcactgcca gtgaacaaac gcgttcccaa
    34201 aattctgttt attctctttg ttgttgcctt ctgcgtttat ttagtgccgc gcgttgccat
    34261 caacttcttc tattatcctg acgataaaat ttacggtccc gacccctggt cggcggaatc
    34321 cgtcgaattt acggctaagg acggtactcg tctgcaaggc tggtttattc cttcttcgac
    34381 gggccctgct gacaacgcca tcgcaaccat cattcatgct cacggcaatg ccggaaatat
    34441 gtccgcccac tggccgctgg tcagttggtt acctgagcgt aatttcaacg tttttatgtt
    34501 tgattatcgc gggtttggta aatcaaaagg cacgccgtcc caagccggat tgctggacga
    34561 tacgcaaagt gccatcaatg tggtgcgcca tcgcagtgat gtaaacccac aacgtctggt
    34621 gctgttcggg cagagtattg gcggggcgaa tattctggat gttattggtc ggggtgatcg
    34681 tgaaggcata cgtgcggtga tcctcgactc cacatttgcc tcttatgcaa ccatcgccaa
    34741 ccagatgatc cccggcagtg gctacttact tgatgagagt tacagcggcg aaaattatat
    34801 cgccagcgtc agcccgatcc cgcttttact cattcacggt aaagctgatc acgttatccc
    34861 atggcagcac agcgaaaagt tgtatagcct ggcaaaagag ccaaaacggt tgatcctaat
    34921 cccggatggc gaacacattg atgctttttc cgatcgtcac ggcgatgttt atcgcgaaca
    34981 gatggtggac tttatcctta gcgcgttgaa tccacagaac taacccatga tcgctagcac
    35041 gataatcatt cacaaaacca ccttaagaca tgctaatcca ctggtcagaa cagtttaaga
    35101 tgagaaaaat tctgtgacgc ttgccaacat ttctgatgat tagcattccc ttcgccattt
    35161 ccctgagcaa actttagcta ttcttatcaa ttatgcttat gggagatcta cagatgatgc
    35221 ctacgcttgc tccaccatct gtcctttcgg ctccccagcg ccgctgccag atcttgctga
    35281 cgctctttca gccggggttg accgccacca cggcaacttt cagcgagctt aatggtgtgg
    35341 atgatgatat tgccagtctt gatatcagcg aaacaggaca ggagatcctg cgctatcatc
    35401 aactcacact gacggctggt tatgacggta gctaccgggt tgaaggtaca gtgcttaacc
    35461 aacgtttgtg tttatttcac tggctacgac gtggtttccg tctgtgtcca tcatttatta
    35521 ccagccattt cacccccgcc ctgaagagtg aactgaagcg gcgcggaatt gcgcgtaact
    35581 tttacgacga taccaatcta caagcgttag tgaatctctg ctcccgacga ctgcaaaaac
    35641 gctttgaaac gcgcgatatt catttcctgt gtctgtatct gcaatattgt ttgctgcaac
    35701 accacgctgg aattacgcct cagtttaatc cgctccaacg tcgctgggcg gaatcctgcc
    35761 ttgaatttca ggtagcgcag gaaattggac gccactggca gcgtcgggcg ctccagcctg
    35821 taccgcctga tgagccactg tttatggcac tacttttttc catgttgcgg gttcccgatc
    35881 cactgcggga tgcgcatcag cgagacagac aattgcgtca gtctatcaaa cgtctggtaa
    35941 accattttcg tgagctggga aatgttcgtt tttatgatga acaggggtta tgtgatcagc
    36001 tttataccca cctcgcccag gcgttaaatc gcagtttgtt tgccatcggt attgataata
    36061 ccctgccgga agagttcgcc agactgtacc cacgcctggt gcgcaccacc cgcgcggcgc
    36121 tggccggatt tgaaagtgaa tacggcgtcc atctttctga tgaggaaagt ggtctggtcg
    36181 cggtgatttt cggtgcctgg ctaatgcagg aaaacgatct gcatgaaaaa cagattattc
    36241 tactgaccgg gaatgatagc gagcgagaag cgcagattga gcagcagtta cgcgaactaa
    36301 cgttactgcc gctcaacatt aagcatatgt cggtaaaggc atttttgcag acaggcgctc
    36361 cgcgtggcgc ggcactgatt attgcgcctt ataccatgcc attaccgctc ttttcaccac
    36421 cgctgatcta tacggacctg acgctgacaa cacatcaaca ggagcagatc cgcaaaatgc
    36481 tcgaatcagc atgagggaac aactttcggg cgcaaaaaca ttgccggcag cgccaccagc
    36541 gccattaccc agaacacgcc gtggcccaga tattgataca ggaaaccggc gaaaacggtc
    36601 atgatagcga tactgccgcc catcgcgacg gcagagtaaa ccgcctgtaa acggatgact
    36661 tcgctaccct ggcgagcagc aatataacgc atggcggcca ggtggcagac cgtgaaggtg
    36721 ccgcaatgca gaatttgcac cactatcaac cacggcaacg ccgtagttgc tcccataatg
    36781 ccccagcgca ctacgccgca aatcgccgag atcaacagca tatcgcgtgc actacaacgg
    36841 cggaaaagtt tattactcag cgcaaagata atgacttccg ccaccacgcc cagcgaccac
    36901 aaatacccca ccgccgaggc cgagtagcca gctgcctgcc agtaaatggc gctaaaaccg
    36961 taataggccg catgtgcccc ctgcaataaa caaacgcagg ccagaaagcg ccagttctgg
    37021 cgaaccagcg ccaaccacgc agaccaaccg gtgctctcct gctggcgact tgccccttgt
    37081 ggctgaatcg tcggacggat gagaaagccg agcagcatgg atgccacgcc caacgtcaac
    37141 agcgcgagga tcacccgata atcaaacata gtgaccagtt tgcccgtcag cgccgagcca
    37201 atgacaaacg ccaccgagcc ccacagtcgc actttgccgt aatcaagcgg gaactgcttt
    37261 tgccacgtat tcgccagtgc atcggtcaac ggtaccagtg gtgagaaaaa gaggttaaag
    37321 ccaatcatca ccagcatcag ccacgctacg tgcgcccccg cccagaaggc gacagcaaag
    37381 agaagtgtca gcagtgccag cacgcgcaag gcggaaatca ggcgggaagg atcgctgacg
    37441 cggggcgcga tgagcaaact cccgaggaaa cgggcaacca gacctgcccc caataacagg
    37501 ccgatggttt ctggcgttaa accaatccct ttaagccaga cgctccagaa aggtagaaaa
    37561 atgccgtaac taaaaaagta tgtgaaatag ccgagcgcca accagcgcgt ggattgcaaa
    37621 accatgagtc cctcccgtca ggaggcgtta gtctggggtt aatcgaatgt tggtgcaagt
    37681 tgaaaaataa gaatattcga gcctgcttcc cgatgtctgt aacgccagtt cacaagcact
    37741 aaaaaactgc gatccgcgac gcatgttgca aaaataatgc gtaattatgc cgttacgctt
    37801 gccaaacgtt cctgcacaat ggcgatgaaa tcgcgtaatg ccggcttcat ctcccctttc
    37861 ttccacgcca tcagtaaagc aatggaaggc acatttccgg caatgggacg aaaaacaacc
    37921 tgcccggtat taaaattatt catataaccg ggtatcaaag tgacgcccag ccccatgccc
    37981 accagattca tggtcaccag aatattcgtt gccacctgga cgatatttgg ctggctgttt
    38041 tcttgcgcaa accacgcttt aacgatcggc gcaagcgaac cggaatacgc cggatcggta
    38101 ctgacgaaat tcacgccatc cagttgcgcg gcggtgatct ctttttcatg tgctaaaggg
    38161 tgatcaaccg gtaacacaac cactaatggt tcgtcaaaaa gctccagata atcaatctcc
    38221 gggctataaa caggatggcg cattaagccg acgtcgagtt caccacggcg aattttttcc
    38281 tcctgttgcg tggtgattaa actcaccagc tcaatcaagg tgtctggctg tctgagacga
    38341 aacatcggta atacttttgg cagtaaattc acttccgccg atggcacaaa gccaatggtt
    38401 aattgtctgt cttcctgaac aattttccgc gcccgtaatt tggcattttc cgcttgttcg
    38461 aggattgcca gcgcgtcctg gagaaaacat tctcccgccg ccgtcaacgc gactttgcgc
    38521 ttatccctca ccagtaacgg aacaccgaca cagttttcaa gatcgcggat ctggctgctt
    38581 aacgaaggct gtgaggtatg cagtttttcc gccgcacggg taaagttcag tgcctgcgcc
    38641 actgcgacga aatagcgtaa atgccgtagt tccatcacct tccccttgtt atcgaaaaaa
    38701 cgtctcagct ggtagaaatt aactatttca catattagca accaaccagc aacatcctta
    38761 tggcacaaaa atagaaggtc aatacatctt atctttcagg attaaaaaat atgaccacac
    38821 cctcagattt gaacatttac caactgattg acacccaaaa tggtcgggtc actccgcgta
    38881 tttataccga cccagatatt taccaactgg agcttgagcg tattttcggt cgttgctggc
    38941 tatttctcgc ccacgaaagc cagatcccaa aacccggtga tttctttaac acctacatgg
    39001 gagaagatgc ggttgtcgta gtgcgtcaga aagacggcag catcaaggcg tttctcaacc
    39061 aatgccgcca ccgggccatg cgtgtgagtt atgcagattg cggcaacact cgcgccttta
    39121 cctgcccgta tcacggctgg tcttatggca ttaacggcga gttgatcgat gtaccgctgg
    39181 aacctcgcgc ctacccacaa gggttgtgta aatcccactg gggactaaac gaagttcctt
    39241 gtgtggagag ttataaaggg ctgatttttg gcaactggga taccagcgca ccgggcctgc
    39301 gtgattacct gggtgacatt gcctggtatc tggatggcat gctggatcgt cgcgaaggcg
    39361 gcaccgaaat tgtcggcggc gtacaaaagt gggtgatcaa ctgtaactgg aaattcccgg
    39421 cagagcagtt cgccagtgac cagtatcatg ctctgttcag ccatgcttct gccgttcagg
    39481 tattaggggc aaaagatgat ggcagcgata agcgcctcgg tgatggacaa accgcccgcc
    39541 cggtgtggga aaccgccaaa gatgcgctgc aatttggtca ggacggtcac ggtagcggtt
    39601 tcttctttac tgaaaaaccg gatgctaatg tctgggtcga tggcgcagtt tcaagctatt
    39661 accgcgaaac ctatgccgaa gcagaacaac gtttaggtga agttcgcgcc ctgcgcctgg
    39721 cgggtcataa caatatattc cccacgcttt catggctaaa cggcactgcc acactccgcg
    39781 tctggcatcc gcgcggccct gatcaagttg aagtgtgggc gttctgtatt actgacaaag
    39841 ccgcctccga tgaagttaaa gccgcttttg aaaacagcgc cactcgtgct tttggtcctg
    39901 ctggttttct cgagcaggat gactcggaga actggtgtga aatccagaaa ttgcttaaag
    39961 gccaccgcgc ccgcaacagc aaactgtgtc tggaaatggg gcttggtcag gaaaagcgcc
    40021 gcgacgacgg cattcctggc attactaact atatcttttc agaaactgcc gctcgtggaa
    40081 tgtaccaacg ctgggccgat cttctgagta gcgaaagctg gcaggaagtg ctcgataaaa
    40141 ccgccgctta ccagcaggag gtgatgaaat gagtgcgcaa gtttcactag agttacatca
    40201 ccgcattagc cagtttctct ttcacgaagc cagcttactg gacgactgga aatttcgtga
    40261 ctggctggcg cagctcgacg aagagattcg ttacaccatg cgcaccacag ttaacgcgca
    40321 aacacgcgac cgccgcaaag gcgtccagcc accgacaacc tggattttta atgacaccaa
    40381 agaccagctg gagcggcgaa tcgcccgtct ggaaacgggc atggcatggg cagaagagcc
    40441 gccgtcacgc acccgccact taatcagcaa ctgccagata agcgaaaccg acatcccaaa
    40501 cgtatttgct gttcgggtga attatctgct ttatcgggca caaaaagagc gcgatgaaac
    40561 gttctatgtc gggactcgtt ttgacaaagt tcgccgtctg gaagatgaca actggcgctt
    40621 gctggaacgg gatatcgtcc tggatcaagc ggtaatcact tcccataacc tgagtgtact
    40681 gttctgatga atcgaattta tgcgtgtccc gttgcggatg tgccggaggg tgaggctctc
    40741 cggatcgata cctcgcccgt catcgccctg ttcaacgttg gcggcgagtt ttatgccatt
    40801 aacgatcgtt gcagccatgg taatgcgtca atgtcagaag ggtatctgga agatgacgcc
    40861 acggtggagt gcccgctaca cgccgccagt ttttgcctga aaacggggaa agcgttatgc
    40921 ctgcccgcca ccgatccgct caccacttat ccagtacacg ttgaaggtgg tgacattttc
    40981 atcgacttac cggaggcgca gccatgagcg atctgcataa cgagtccatt tttattaccg
    41041 gcggcggatc gggattaggg ctggcgctgg tcgagcgatt tatcgaagaa ggcgcgcagg
    41101 ttgccacgct ggaactgtcg gcggcaaaag tcgccagtct gcgtcagcga tttggcgaac
    41161 atattctggc ggtggaaggt aacgtgacct gttatgccga ttatcaacgc gcggtcgatc
    41221 agatcctgac tcgttccggc aagctggatt gttttatcgg caatgcgggc atctgggatc
    41281 acaatgcctc actggttaat actcccgcag agacgctcga aaccggcttc cacgagctat
    41341 ttaacgtcaa tgttctcggt tacctgctgg gcgcaaaagc ctgcgctccg gcgttaatcg
    41401 ccagtgaagg cagcatgatt ttcacactgt caaatgccgc ctggtatcct ggcggcggtg
    41461 gcccgctgta caccgccagt aaacatgccg caaccggact tattcgccaa ctggcttatg
    41521 aactggcacc gaaagtgcgg gtgaatggcg tcggcccgtg tggtatggcc agcgacctgc
    41581 gcggcccaca ggcgctcggg caaagtgaaa cctcgataat gcagtctctg acgccggaga
    41641 aaattgccgc cattttaccg ctgcaatttt tcccgcaacc ggcggatttt acggggccgt
    41701 atgtgatgtt ggcatcgcgg cgcaataatc gcgcattaag cggtgtgatg atcaacgctg
    41761 atgcgggttt agcgattcgc ggcattcgcc acgtagcggc tgggctggat ctttaaggaa
    41821 gcacgatgaa agaaaaaacg atcattattg tcggtggcgg gcaagcggcg gcaatggctg
    41881 cggcctcgct acgccagcaa gggttcaccg gtgagctgca tctgttttcc gatgagcgac
    41941 atcttcctta tgaacgacct ccactctcga aatccatgtt gctggaagat tccccgcagt
    42001 tacagcaggt gttacccgct aactggtggc aggaaaacaa tgttcatctg cattccggtg
    42061 taaccatcaa aacgctgggt cgcgacacac gagagttagt gttaaccaac ggcgaaagct
    42121 ggcactggga tcagcttttt atagcaaccg gcgcggcagc tcgaccgctg ccgttgcttg
    42181 atgcactggg agaacgctgc tttaccctac gccatgccgg tgatgccgcc agactgcgag
    42241 aagttctgca gcccgaacgg tcagtcgtga ttgtcggtgc cggaactatt ggtctggaac
    42301 tggctgccag cgccacgcag cgcagatgta aggtgacagt gattgaactg gcggcaaccg
    42361 tcatgggccg taatgcacca ccgcccgtgc aacgctatct tttacagcgc caccagcagg
    42421 ctggtgtgcg cattctgctc aataatgcca ttgaacatgt ggtcgatggt gaaaaagtag
    42481 aactgacgct gcaaagtggg gaaacgcttc aggctgatgt ggtgatttac ggtattggta
    42541 tcagcgccaa cgagcaactg gctcgcgagg ccaaccttga tactgccaat ggcattgtca
    42601 ttgatgaggc ttgccgcacc tgcgatcccg cgatctttgc cggtggcgat gtggcaatca
    42661 ctcgtcttga taatggtgca ctacaccgct gcgaaagctg ggaaaacgcc aataaccagg
    42721 cgcaaattgc cgctgccgca atgttagggc taccgctacc gctactgccg ccgccgtggt
    42781 tctggagcga tcagtacagt gataacttac agtttattgg cgatatgcgt ggcgatgact
    42841 ggctttgtcg tggcaacccg gaaactcaga aggcgatttg gtttaatctg caaaacggcg
    42901 tgcttatcgg tgcggtcacg ctgaatcagg ggcgtgagat tcgcccaatt cgcaaatgga
    42961 tccagagcgg caaaacgttt gatgcgaaac tgctgataga tgagaacatc gcgcttaaat
    43021 cactgtaacc aggataatta gcgaatatct caatgcctgg ggcgtggcga ggtgcaagag
    43081 tgtgtattac gtttaaatca cattatcttg caaagggatt ggttatgaac acactacgtt
    43141 attttgattt tggagctgcc cgccccgttt tgttattaat tgcccgtatc gccgtggtct
    43201 taattttcat tatttttggt tttcccaaaa tgatgggctt tgacggtacg gtccaatata
    43261 tggcctcgtt gggcgcgcca atgccgatgc tggcagcgat tattgcggta gttatggaag
    43321 tgcccgccgc gatattaatc gtgcttggct ttttcacccg tccgctggcg gtgctgttta
    43381 ttttctacac gctgggtacg gcggtgattg gtcaccatta ctgggatatg accggcgatg
    43441 cggttgggcc aaatatgatt aatttctgga agaatgtcag tatcgctggc gcattcctgc
    43501 tgctggcaat taccgggccg ggggcaattt ctctcgatcg gcgttaggaa agatgccgga
    43561 tgcggcgtga acgccttatc cggcatttaa taaattacag ccactctacc cgcaacgaca
    43621 tctctgaagt tgttgattcc cctggtgcta gcgcaatgag atcaccccct tccgggcgat
    43681 gatgatcatc cggcgcatgg ctcatcggtt ccagacagaa gaaatcaaac gcatatcctt
    43741 tatcaaacgc agggtcggaa acaaagatga aataacacgg tgcaggtggc gtcgtttcca
    43801 tgatgatggc ataaccttcc tgcggctgct cgatgcgggc ctgaccattc catccggcaa
    43861 aaccattgtt cacccactgg cgcggcaacg gcgcgggctg gttaaaatcc agttcctgcg
    43921 gtagctgctc gcaaaactca cccgccagcc actgctcccg ctccagccag taaccgctcg
    43981 cctgtgcctg aatccgcgtt tgcggcgaca acggaaaata aggatgccag ccggtaccaa
    44041 atggcagcgt ctctgcccct tgattggtga cagagagcgt caccgtcagc gtatccgccg
    44101 ttaaatgaaa cgcctgactt acccgatagt gatagacacc gctgcgatgt tcatacacca
    44161 gacacaaact atcatcgctg tgcgagacac attgccactc gcccagccag ccatcgccgt
    44221 gtagatagtg cgcatcccac tcaacattcg gttgcagttg atactcacgc ccctgccaga
    44281 caaaacgatt gccgctcacc cggtttgcaa atggcaccag cggaaaacat gaggcatcgg
    44341 ttgccacacc gcttttttta ccaggacgta agagcggcgt cgtatcgcgc cagaagcctt
    44401 cgattacgcc gccctggtca gaaacgtcca gctttagcga cccgtgggat aaggtataga
    44461 tggtcatccg ctaactcctt aatccgggaa gttaatcaca actttcccgc atttgccgct
    44521 cgccatcagc gcataggcat cacctgcctg ttccagcgag aagcgatggg taatggcgtt
    44581 acgcggccac agtttccagt ccgtcagatc atgggcgcat ttttccatat ggaacagact
    44641 ggtcacccag gagccgataa tccgccgttg atggtgcatc agatcggcgc tgacctcgaa
    44701 ctccactttt ccggtttctc cgatgtaaac cacccgtccc cagtcagcgg tggatcgcag
    44761 tgccagcaag cgacctgcgg cattaccaga acaatcgagc gcaacatccg cgccaccgtg
    44821 ggtgagttcg gcgataatct gcggcagacc ttcggtggtt gctaaatagc cgtgatccat
    44881 cacccctaac tgttttgcca tcgccagacg ttccggcagc atatcaacgc cgatgatccg
    44941 ttttgcaccg cgacctttcg ccagcatcat cgccatcatg ccgactggcc ccagaccgac
    45001 caccagcacg ttatcactgc cggaaacttc gccgcgcaaa atcccttcat aggccgtgcc
    45061 aacgccgcaa ctgataaacg caccatcttc gtagctcagc gcatccggca ggaggatcag
    45121 atctttttct tccgccagca gatattcggc atgaccgccg tcacgctgcc agccgtaagc
    45181 cgcttttcct ttgccagtac aagagatagg aaagccgcgg cggcagttcg ggcaaaaacc
    45241 acagccagaa atgtgataca ccagcacgcg atcaccctct ttaaaatggc gacagccttg
    45301 ccccatcgcc acaatctgcc cgcacggttc atgaccgttg ataaatccct ggtataacgg
    45361 tttatcgggt gccgccgccg tggcacggtg ttgatgatag atatagtgga catcgcttcc
    45421 gcaaatcccg gaggatttca ttttgatcag tacctggtta atccccggcg tcggcaccgc
    45481 aacttcccgc agatcgacgg ttgaatttcc tggtaaataa gctgccagca tcgttttcat
    45541 aaatccctca ttaacaatac gattaatttt catccctgcc gcacccgcgc caggggcgtt
    45601 aaggtttagc gtttcgcttt actgcttcgc tgggtcagca agatattcgc cagcaccgcc
    45661 accacgatga tgacgccgcg taccacctgc tggaaaaagg agttaatacc gagcagcacc
    45721 agaccgttac cgattagcgt aatcaccagc acaccaagca atgtaccgaa cagggaaccg
    45781 cgaccgccgg aaagtgccgt accaccgacc acgaccgcgg cgatgacgtc aaactccaga
    45841 ccgtttgcgg cacctgcgtt accagaaccg aggcgcgccg ccaacaaaat gccggtcacc
    45901 gccgctaata atcccgaaag ggtaaagata agaatgcgca cccgacgaac gttgatgccg
    45961 cacaactgcg ccgccgtagc attaccgcca acggcaaaaa ccgagcgccc gaaggcggtt
    46021 ttgcggctga tgaacacaaa cagcgcaaac aacacaatca tgatcagcgc ggataccggc
    46081 acaccgagaa attgtccgcc cagccagtcc agcacctcgt tttcgtcaat cggcactggc
    46141 agtgcgttcg tcataaacag ccccattccg cgcagggcgc tccacagccc cagtgtggca
    46201 acgaaacttg gtacgttaaa cacgccgcgc agcaccccgg ccagcgttcc catcagcgcg
    46261 cctaacagca acaccagcag acacgccacc gccagcggaa cttcaaattg cagcaaaaat
    46321 gccaggcaca ccgagacaaa agccaccatc ggcccaacgc tgacatcaat ttcaccggag
    46381 ataataatca gcgtcatcgc ccaggcggca atcccaatgg tggcggcatc gcgcagcacg
    46441 ttcatctggt tattcaatga gataaagcca ggcgcgttca gggagaagac cagataaaga
    46501 atggcgatta ccaccagcaa accgatctca ttaatatggc gactgacaaa ttgtttgagc
    46561 gagacgctct tgccctgcgg caatgataat gacgaagcag acatcttcag gttcctcggt
    46621 aaaattcatc agtgcacaga cagaatggcg gacatcagct catccacatt gaccggcgag
    46681 tgaaactcct gcgagaacgt accgtgctgt aacaacagga tgcggtcgca caccagcggc
    46741 aactcctcca cttcactgga gataaacacc acgctttttc cttcggcagc cagctcacgg
    46801 acaatacggt aaatctgctg tttggcttcg atatcgacgc cgcgcgttgg ctcgtcgagc
    46861 aacaaaatct ggctggcggc atagacccaa cgaccgatca ccactttttg ctgattgccg
    46921 ccagaaagcg tgccgatggg cgtttcgcta ctggcggcct tgaccgtcat ccgctgcatc
    46981 acctcttcgg tcaggcggcg gatggtggac cattgcagta cgccgttggc gctgattttt
    47041 tgccgattgg tcagcactgt attttcgtca acgcccaacc agggaatgat ccccgcttct
    47101 ttgcggtttt ctggcgtata gccaatgccg cgtttcagca tgtcgccgta atcggggcgc
    47161 gtgattttct cgccgttgat aacaatttcg ccctgttcat actcctccag cccaacaatc
    47221 gccttcagca attcactgcg ccctgccccc agcaggccag caatgccaag cacttcgccg
    47281 cgacgtaacg taaagctgat atcctccagc ttgggcttat ggcgtaatgc acggacttcc
    47341 agcacggcct gatccacaat ttcctgaggg gctaccggcg caatatcaac gtgatcacgc
    47401 cctagcatta gcgacacaat atgatgcgtg gaggtgtttt cgagcatcac atcgcccgcc
    47461 acctgaccat cgcgcataac ggtggcacag gaggcaatgc ggcggatttc ttccatccgg
    47521 tggctgacat aaatcaccgc cacgcccagt gccgacatct ttttcaccgc actgatcacc
    47581 agttcaactt ctgcactggc aagcgagctg gtgggttcat caagaatgac cacgcgcggc
    47641 tcgcccttca tcacccgtgc aatttccacc agttgctttt gcgccgggct tagcgttgaa
    47701 acaagttgtt caggactaac gtcaacgccc agcgcctgta agcaacgttg ggcatcctgc
    47761 gccatttgca ggtaatcaat catgccgttg cggcgaggcc attgaccaag gcagaggttt
    47821 tctgccaccg tcagcccttc caccagactt aattcctgat aaaccgcacg aacccccagt
    47881 tcagcggcac ggcgagtcaa cgtagcttcg tcaccttcca gtcgcgtctc gccaatccag
    47941 atatcaccgc tatccgggcg ttcgctgtcg gtaagcattc gaatgagagt cgatttgccc
    48001 gcgccgtttt tacctaacag cgcacgaact tcgcctttat tgagcgtgaa gttaacgtta
    48061 tccagcgcaa cgacgccggg gtaacgctta tttcctgcca ccacttttgc taccgggact
    48121 gcctctgttg ccgtgaacat agggacctct gcgaatcagc gattagggca gaccatcaac
    48181 gtgcgttgcc agccactgtt taccatcttc cgttttggta tagagatcga ttggcacctg
    48241 aatcactttt tcaccgtcgg cttgtttatt gataaccttc aatgtttgcg cgaaaacagc
    48301 attgcccatt ttcttaccgg aaatatccac taccgctttc agcacctgat tgttttccag
    48361 ctcctgagca atttcggttg tcatatccga accgaaaaca gcaatttttc cggcctgatt
    48421 ttgattacgt accgctttta ccgcgccgag tgtcgcaccg cccgactccc ccataatggc
    48481 gttgagatcc ggcgtggaga taatcagttt ttcaccaacg gaaatcgctt tatctaaaac
    48541 agtcccttcc tgattagcga caatttgcgc gccgggaacg cgggatttta atacttcttc
    48601 aaatccttta cgtcgctgca cacaaacttc aaaggcttcg caattgatga cggcaatttt
    48661 cggctggtca attttattgg caataaaata atcggcggca gcgttaccca gttttttacc
    48721 aaattccagt ggatcgccga ccagatacgc cgagacatat ttatcgacac ccttttgatt
    48781 aatacaggtg ttgtagcaaa tcaccggaat acccgcttca ctggcgcgac gaacggtacg
    48841 gctactgcca ttttcagaca ctgccgataa aataatggca tcgacattac gcgccacgag
    48901 ggtatcaaca aaggtacttt ctttcgaaat atcaccctgg gcgttagttt caattaactg
    48961 tacctgtact gaagaatctt ttgccgcatc ctgaacgccc tgacgcactc cagcgtaata
    49021 tccctgggta tcaaggtata ttgcaccaat ggtcatttct ttatccgcag ccctggcaaa
    49081 tagtgcgcta cctaatagcg tacccattaa taatagatta cgggttgttc tcattttttt
    49141 aggcatagtg cctcctgtag ggtttttatt aacaacggct tattctaatt attttgtgat
    49201 gagcggcagc gcgtgccgct caaatattac agttaatagg gtttagtgaa cgtagttaaa
    49261 aataaaaggc atcacggtgg tgaataatgc cgctttcggc caggccggat taatcgccgt
    49321 caattcagcg cgagcagatt catattgcgc aacctccccg agtcccgcgg acgccagcat
    49381 cgccaccatc aggcattttt ctttatgctg ctgttgtaaa tcgccataca gcgacaacag
    49441 gtcaggttgt gaaacagcaa agaaatccgc ctcaatactg gttttcgcca tctcttgcgc
    49501 ccactgtttc atttcactaa acagttgctg tgcggtttgc tgttcaccca gcagtcgcag
    49561 cgccattcct tgccagaaga gataatcaac cggctgatcg ttgtaataac tgtggatgtt
    49621 aatggtgcga tcgccggtcg ccgccagacg taaacaacgc atcgcttcag tttcatcgcc
    49681 ctgcgcgttg gcgcatatcg cctgccagaa ccagatgtcg ttatcagttt gccccggtaa
    49741 acggccttcg cttaaattct ccggataatg cagcgcggca tgaagcagtt cgctggcctg
    49801 ctgcggctct ctggcatcaa gatgctgcca ggcgcgtaat aactggttga ggataaactg
    49861 actggtgacc ttcccttccc cgccttccca cgggtggaat ttgcgcgtgg cgagaatgtc
    49921 cgccgctttg tctgcctgac ccgtgagatg ccacaaattg agcaactctg ctgtcatgtc
    49981 gtcgcgtttc agcgcaattt ccagattatt ttccagacgc gccagtcgtt tctccggtgt
    50041 ggctccgctt aacttatcaa gcaaatcccg ttcgaaaagc agacgtgcat cctgcggcgc
    50101 aagctgataa gcattatcaa gataacgcgc ggccagctca taatcgtgtt gcttattcca
    50161 cgcatggatc gctaacccgc gccagccgtc ggcaaactcc ggcgacatct ctacgcaacg
    50221 ttgccataag gtaatggctt tgttgtagct acgcttgttg tagtagaagc aggccagtaa
    50281 atgacgggca aaccagcact cttcaatact ctccagcgcc gccacttctt ccagcgtatt
    50341 cgggaaacgg acaaactgcg ggaagacatc aatggctttt gcgaccagtt cgccgcgttc
    50401 ggctttcggc aataaactcg cctgcaggta aagcggcagc gtgcgctggc agtccagtgc
    50461 gttcagcatc tctgccgcca gggtgggcat tccccagtta atcagttgcc cggcggtcat
    50521 cagagcgtta acgtcgcgtc cctgacacag accgcgccac tgcgcgagag ctgattcgct
    50581 acgaccatcg aaccagttca gccaccacag agtggcgttc agcggataat cgcgcagcag
    50641 tttctcgcgc tgcacacgcg cgttgtcctg acgaccactt aacaccagca gcagattatg
    50701 caggcaaagc acttcctgat tggttgggca ggcgcgaaga ctttgttggc aaaaatccag
    50761 accagcgtcg aagttaccat tacgcgccgc cagtcgtgcc aggccataat agccaccggc
    50821 tttgctgttg ccgctccaga ccgcacgcca gaaatcctct tcggcttgtt gatattgtcc
    50881 ctgacgttcg taagcactgg cgcgaatcaa actggcctgt ccgcactgcg gatttttgtt
    50941 cagcgcatgt gcgcgtttca gcgcctgagt ggcatacgcc accgcttgcg ggaaatcggc
    51001 gcggttatat tccagcatcg ccagcgccag gttacagcgg taatccagcg gatccagcac
    51061 cacgccgcgc aggtagtaat cgaacggtga acggctggcg tgatgatatt gctccagatg
    51121 ctgaccgata aaccaggctt catctgtact ggtaatgtct tgtgctgcca gtggcgcttt
    51181 ggcgacgtcc ggcaacggca actcttgcgg ctgatgttcc tgataactca gtacaatatt
    51241 gccgtcggca tcggagagct caatggtcag cctttcagga ttgataccgt gcaacacgcc
    51301 ctggatggcg gtcgcaggca tcagtgccac ggcatcatca agtaacgcgt tgcatttgcc
    51361 gatttcgcgg atcgccaggc ggtatccgtt caacggagag atggcataca gcccccactc
    51421 aatcccccgc ttactacgct ggagttttat caccgcatcg cgggaggcat tttgcaccat
    51481 gcccaaagaa tgataaggca ggaaatactg ctcaaaacgc ttctcttcgt aagcatcaag
    51541 ccaggtaaaa tcgggctggt tatcggcaaa aataccggtc atcagttcga tatacgggcc
    51601 gttattgtcg gtcagactct tatcccacgc ctggccaaat tcactgtgtc cccaactcca
    51661 ctgtttttta cctggcgcga tatggtggtt ggcaacgtgc agcaaaccgc catcttcatc
    51721 gtgacaccac gcgccgacaa aatcgtactg cgatttttcg gccatatatg aggttggaac
    51781 aggcacattt ttatagcgag aaatgtccac tccggcggag tagtccactt tgtagtaagt
    51841 gccggtggcg atggggaaag cggagacggc ccgtttgccg tgatcaaaca ccgcagtcac
    51901 atccggtggg aaaacgctct gatgcccttc cccccctttc actgccgggt tggcccacca
    51961 caagaaatga cgcggcgtgg cgttaccgtt atagacgcgg ctggcgattt ccagcgccgc
    52021 ccggtcaggg cgcagggtga aacctgtcat cacctgtaaa ccatgcatcg gctccgtttc
    52081 gcctacccac accgtctgtg caccgtcttc atgggcttcg agggtgaaat caacgggcat
    52141 aaaggtggtc gggcgatgat gttgcggcca gttaaactca atcccaccgg aaatccacgg
    52201 ccccagcagc cccaccagcg caggtttaat gacttcattg tgataaacaa aatcgcgctg
    52261 tttcacttta tcccacgcgc gatgcacccg accgcccagt tccggcagga tcatcacttt
    52321 gatgtagtcg ttttccagcc acaccgcctg ccaggatttc agggttttct gctcgctcag
    52381 cgtatcggtc acgccgtagg gataaaccgc gccggacgat ccctgataaa cgcgattttc
    52441 caggaacatg ggatgtatat cctgcggccc ggtttcatag gtcgggatct caacgcgctc
    52501 ttgccacact tttactggag tcatggtgcc ctcaatatta acaagacata ctgaattaaa
    52561 agatttgtgg cagtgtattg aacaatctgg caatgttttc gcggaataat cacgcaatta
    52621 actaaacaag gtttagtgaa gatgagagcc tgcattaaca atcaacagat tcgccaccat
    52681 aacaaatgcg tgattctgga actgctgtac cggcaaaagc gcgccaataa atcaacgctg
    52741 gcccggctgg cgcaaatttc gattccggca gtcagtaata ttttgcagga actggaaagc
    52801 gaaaaacggg tggtgaatat tgacgatgaa agccagacgc gcgggcatag tagcggtaca
    52861 tggctgattg cgccgaaagg tgactggacg ctgtgcctga acgtgacgcc caccagtatt
    52921 gagtgtcagg ttgctaatgc ttgtttaagt ccgaaaggtg aatttgagta tttacagatt
    52981 gatgcaccga cgccgcaggc gctgctgtcc gaaatcgaaa aatgctggca tcgccaccgt
    53041 aaattgtggc cggaccgcac catcaatctg gcgctggcaa tccacggtca ggttgatccg
    53101 gtgaccggcg tgtcgcaaac catgccgcaa gcgccgtgga caacaccggt tgaggtgaag
    53161 tatctgctgg aagagaagct gggcattcgg gtgatggtcg ataatgactg cgtgatgctg
    53221 gcgctggcgg agaaatggca aaataattcg caggaacggg atttctgcgt gatcaacgtt
    53281 gattacggca ttggctcgtc gttcgtgatt aacgagcaaa tttatcgcgg cagtttgtat
    53341 ggtagcggac agattggtca caccatcgtt aatccggatg gcgtcgtctg cgactgtgga
    53401 cgttacggct gcctggaaac cgtcgcctcg ttaagtgcat taaaaaaaca ggcgcgggta
    53461 tggctaaaat cacaaccggt taatactcag cttgatcctg aaaaactgac aaccgcgcag
    53521 ttaatcgctg cctggcagag tggagaaccg tggatcacca gctgggttga tcgctctgcc
    53581 aatgccattg gtttgagtct gtataacttc ctcaacatcc tcaatattaa tcagatttgg
    53641 ttgtacggtc gcagttgtgc ctttggtgag aactggctta atactattat tcgccagaca
    53701 ggatttaacc cgttcgaccg cgacgaagga ccgagcgtga aagcgacgca aattggcttc
    53761 gggcaattaa gccgcgcaca acaggtgctg ggaattggct atttgtatgt tgaggcgcag
    53821 ttacgacaga tttgatggcg cgataacgta gaaaggcttc ccgaaggaag ccttttttca
    53881 acagattaac cgtttcgctt atgcgtaaac cgggtaacgt gcgcagatgt cgagaacttt
    53941 acctttgatg cgctcgataa cggcttcatc attgatgctg tccagcacgt cacacatcca
    54001 gccagccagt tctttcgctt ctgcttcttt gaagccgcga cgcgtaattg ccggagtacc
    54061 cacgcgaata ccggaggtca caaacgggct cttcggatcg ttcggtacgc tgtttttgtt
    54121 gacggtgatg ttagcacggc ccagagcggc gtctgcttct ttaccggtca ggtttttatc
    54181 aaccagatca accaggaaca ggtggttatc agtgccgccg gaaaccactt tgtagccgcg
    54241 ctcgaggaac acttctacca tcgctttagc gtttttcgcg acctgctgct ggtaagtttt
    54301 gaactcaggc tccatcgctt ctttcagagc aaccgcttta ccggcgatta cgtgcatcaa
    54361 cggaccgccc tgaccaccag ggaaaacggc agagttcagt tttttgtaca gctcttcgct
    54421 accacctttc gccaggatca ggccgccgcg cggacccgcc agggttttgt gagtggtggt
    54481 agtaacaacg tgagcatgag gaaccgggtt cgggtagacg ccagcagcaa ccaggcccgc
    54541 aacgtgcgcc atatcaacga acaggtaagc accgatgctg tcagcgattt cacgcatttt
    54601 cgcccagtcc accacgccgg aatatgcaga gaaaccaccg ataatcattt tcggcttgtg
    54661 ttctttggct tgtttttcca gatcggcgta gtcgatatga ccggtagcat cgataccgta
    54721 aggaacgatg ttgtacagtt taccggagaa gttaaccgga gaaccgtgag tcaggtgacc
    54781 gccatgcgcc aggttcatac ccagaacggt atcacctggt tccagcagcg cggtgtagac
    54841 cgcaaagtta gcctgggagc cggagtgcgg ctggacgtta gcgtagtcag cgccgaacag
    54901 ttctttcgca cgatcgatcg ccagttgttc aacgatatca acatactcgc aaccgccgta
    54961 gtagcgtttg cccggataac cttcagcata tttgttggtc agctgagaac cctgcgcctg
    55021 cattacgcgc gggctggtgt agttttcgga ggcgatcagt tcgatgtgct cttcctgacg
    55081 tactttttcc tgctccatag cctgccacag ttcggcatca taatcggcaa tgttcatttc
    55141 acgctttaac atccgcatct cctgactcag ctaacaataa aatttttggc ctttataggc
    55201 ggtcctgttg gacaacggcg aacagtataa ccgaatcatt gtgcgataac aggtcttgac
    55261 aaaggaattt acgcaaacga ttaccttcag gctacacaag gctttggaga ataaagagct
    55321 tgcaaccgga aacggatttc ttttcaggtt tgtgatgcaa atttttcact tcatcacatt
    55381 ctttctgaaa aacaccaaag aaccatttac attgcagggc tattttttat aagatgcatt
    55441 tgagatacat caattaagat gcaaaaaaag gaagaccata tgcttgacgc tcaaaccatc
    55501 gctacagtaa aagccaccat ccctttactg gtggaaacgg ggccaaagtt aaccgcccat
    55561 ttctacgacc gtatgtttac tcataaccca gaactcaaag aaatttttaa catgagtaac
    55621 cagcgtaatg gcgatcaacg tgaagccctg tttaacgcta ttgccgccta cgccagtaat
    55681 attgaaaacc tgcctgcgct gctgccagcg gtagaaaaaa tcgcgcagaa gcacaccagt
    55741 ttccagatca aaccggaaca gtacaacatc gtcggcgaac acctgttggc gacgctggac
    55801 gaaatgttca gcccgggcca ggaagtgctg gacgcgtggg gtaaagccta tggtgtactg
    55861 gctaatgtat ttatcaatcg cgaggcggaa atctataacg aaaacgccag caaagccggt
    55921 ggttgggaag gtactcgcga tttccgcatt gtggctaaaa caccgcgcag cgcgcttatc
    55981 accagcttcg aactggagcc ggtcgacggt ggcgcagtgg cagaataccg tccggggcaa
    56041 tatctcggcg tctggctgaa gccggaaggt ttcccgcatc aggaaattcg tcagtactct
    56101 ttgactcgca aaccggatgg caaaggctat cgtattgcgg tgaaacgcga agagggtggg
    56161 caggtatcca actggttgca caatcacgcc aatgttggcg atgtcgtgaa actggtcgct
    56221 ccggcaggtg atttctttat ggctgtcgca gatgacacac cagtgacgtt aatctctgcc
    56281 ggtgttggtc aaacgccaat gctggcaatg ctcgacacgc tggcaaaagc aggccacaca
    56341 gcacaagtga actggttcca tgcggcagaa aatggcgatg ttcacgcctt tgctgatgaa
    56401 gttaaggaac tggggcagtc actgccgcgc tttaccgcgc acacctggta tcgtcagccg
    56461 agcgaagccg atcgcgctaa aggtcagttt gatagcgaag gtctgatgga tttgagcaaa
    56521 ctggaaggtg cgttcagcga tccgacaatg cagttctatc tctgcggccc ggttggcttc
    56581 atgcagttta ccgcgaaaca gttagtggat ctgggcgtga agcaggaaaa cattcattac
    56641 gaatgctttg gcccgcataa ggtgctgtaa tttgatgttg ccggatggaa acatccggca
    56701 acccttgacg cggtttaaat tgccgcgtcg tcctcttcac cggtacggat gcgaatgacc
    56761 cgtgccacgt cgaagacgaa gattttaccg tcaccgattt tgccggtttg cgccgtgcga
    56821 ataatggtat caacacaggt atcgacaatg tcgtccggta cgacaatctc aattttcact
    56881 ttcggcagaa aatccaccat atactccgcg ccgcggtaca gctcggtatg gcctttctgg
    56941 cggccaaaac ctttcacctc agtgaccgtc atgccggtaa tgccgacttc agccagtgct
    57001 tcgcgaacat cgtccagctt gaagggttta ataatcgcat caatcttttt catgctattc
    57061 cttgaaaagg tcgcctgtct tttgatctgc taaacgtaac acataacgcc aattcattcc
    57121 ttgaaatcgt ttgcatccag ctcgtgtcgg gaaagcagtt tataaaattc tgtccggttg
    57181 cgccccgcca ttctcgccgc gtgtgtgacg ttgcctttgg tgatttgcag cagcttacgc
    57241 aaatagttga gttcaaactg attacgtgcc tcaacaaagg ttggcagcgc cgtattttca
    57301 ccctccagcg cctgctctac taatgcatcg ctaataaccg gagatgaggt cagcgccacg
    57361 cactgttcaa tcacgttgac caactggcgc acattacccg gccagctagc ggtcatcagg
    57421 cgtttcatcg catcagtgga gaacgcgcgg acaaacggtt tatgtcgctc tgccgcctgg
    57481 cgcaacaggt gatttgccag aagcggaatg tcttctgtgc gctctgccag agccggaatt
    57541 ttcaggctga caacgttgag gcggtaatag aggtcttcac ggaattcccc gcgcgccatc
    57601 gcttttggca ggtcgcggtg agtggcggaa ataatccgca cgtcgatatc aatatcgcgg
    57661 ttactgccca gcgggcgcac tttacgctcc tgcaacacgc gcagcagttt gacctgcaac
    57721 ggtgcgggca tatcgccaat ctcatcgaga aatagcgtac cgccttccgc cgcctggaat
    57781 aaaccttcgc gattgctgac agcgccagta aacgcgccac gcgcatgacc aaacagctcc
    57841 gactccagca attgctcggg caatgcgcca cagttaatag caataaatgg tttgctattg
    57901 cgcgggctgg cgttgtggat agcctgggcg aaaatctctt tcccggtgcc gctctggccg
    57961 ttaatcaaaa cgctaacgtc tgattgcgcc accagccgcg cctgttccag caaacgcagc
    58021 atcagcgggc tgcgggtgac aatcgcctcg cgccagcgtt catcggtggc tggcgcggat
    58081 tgttccagcg catcgtcaat tgcctgatat agcgcatctt tatcgacagg cttggtgagg
    58141 aaactgaaca cgccctgctg tgttgcagca acggcatcgg gaatagaacc atgcgcggta
    58201 agaataatta ctggcattcc cggctgcact ttctgaattt cagcaaacag ctgcataccg
    58261 tccatttcat ccatccgcag gtcgctgatg actaaatcta ctttttcgcg attcagtacc
    58321 cgtaatcctt cagcgccact ttccgccgtg accacgctgt agccttcgct ggtcaggcgc
    58381 aggccaagca gtttcagcaa tcccggatcg tcatcgacca ataataaatg cgcaggttta
    58441 tggctcatca ggagtgacct catgggtgga tggcgcgggc ttttcaccct cgtgcggcgt
    58501 atccggcgag aaatttccag ccggtttgcg ggtcgagagc tggcgttcaa tatcagtcag
    58561 gttttccagc ttgcgggtgg tgagttccag ttgctgctgt agaacgtgat gttgctggcg
    58621 caatgtatcc agctcgctgt cgctggcctg ctggagcttg ccgtagcgtt ggcgctcttc
    58681 cgccagttgc agttgcagcg cctgaccgtc gcgccagagt tgatacagag ggcgaacctg
    58741 tgccgggatc tcggtactta gcgcatcaat gcgcgcgacc agctggcggc gctcatacgg
    58801 cgtaattttg gcgtcggcga gcaaaatccc ttgtttaaag gtattttgcc agctgccgtc
    58861 gtcatattgg cgagcttgct gacgtgactg cgcaggcatt aaacgatcag cacaatccat
    58921 cgcccgcagc cagtaaagcg gattggtttc ggttgattta ccttgcagcg cccagatgtc
    58981 gctacattca gtagaaagat agtcagccag ttgataaacc ggaatttttt cttctgctgg
    59041 cgtatcaatg gctggcttat tgtgactctg cacgcaaccc agcaatgcca gacatggcag
    59101 ccctgccagc cacagccgtc ggggcattaa tcgttgaaaa atgtgtcgca tattcaccag
    59161 acttaatgcc tatcccagtg ggcgtaatta ttgcaggcag tttggacatg gacagcgcgg
    59221 agaaaccgga gcgtacacgt cgtacgtgag gatttcgagc actgcccggg gccgaactgg
    59281 caaataaaat agcctgatgg gataggctgt tagcattatt tcgtgttttt cgacgacggc
    59341 aattcaatgc ggaaacaaac gtcttgcccg ctctcgtcga ccagatacag ttccccttgc
    59401 atacggcgaa tacaatccct ggcaatgctt aatcccagac cgctgccctt caccgcccct
    59461 tttcgctggt ggcttccttg aaaaaagggt tcgaagatca tggcgcgttc ctcttgcgga
    59521 atgggtgtgc ctgtattgat gacatcaata taaacccgtg caccatgtaa gctgctgcga
    59581 aggcaaatgt taccggattc agccccgtag tgcaccgcat tggagtaaag attatccagt
    59641 acgctcatca gcagcattgg ctccgccagg caagctgttg ctttgagatc gacgtcggta
    59701 tgcatcattt tagcccgtgc gggcaggcta tgagcagaaa ctactgtctc caccagcggt
    59761 gctaactcaa cattctccag ttccaccgca ctgtccgcct gtttacggtt gtaatcaagc
    59821 agttgttcga tcagtttttg caaattgcgg ctgctgctat caagaatgct caccacctct
    59881 ttttgctctg gcgtaagcgg cccgacaacc tggtcagcca gtaattcagt gccctcgcgc
    59941 atactcgcca gtggcgtttt taattcatga gataaatgtc ttaaaaattg atggcgttgg
    60001 gattccagcc atgacaggcg ctcacttaac caaagaatac gttgcccaac cgagcgtaac
    60061 tcgctcggtc cactgaacga aacgctattg cccagagaac gcccttcccc cagacggttg
    60121 atcatgcgct cgatattttt caccggcccg ataatcatcc gcgtgaaaag tagcaccatc
    60181 accagactca ccagaaatag caccagcgat tgccaaccaa aatattgccc acgttcggcg
    60241 atttcacgct gaagttgctg cccacgagag aacaccactg tgcgcgtggc ctgcaccatt
    60301 tcggtattgg cactggcaaa ggcttcaaga cgcgcggcgg cggcagcatc gggaccactg
    60361 ttgttacact gaagttgggc cagattgttc aaatcctgac gcaatgcctg gtaaagttta
    60421 tcgtctggca gtacgcctgc gtgggcatcg agcatttcgc tgtaacgctt gcgctggctt
    60481 tgataaacct tcgcaagcgt agggtcgtcc agcacgcaat actgacggta gctacgctcc
    60541 atctccagcg ccgcgttggt catcgcttca ctgcgccggg cgtcaataag cgtggtgcgg
    60601 ttaaccagcg ccgcctgatc gctaagcgca ttcaggcttt gccaggcttg ccatgccagc
    60661 accaacaagg gcagcagaat tagcaaaaat gccagcatta ccagttgtcg taatgagcgg
    60721 ggaaaaacgg gccagcgttt caaggtgtta ctctcgtcag acgcgaatag cctgatgcta
    60781 accgagggga agttcagata caacaaagcc gggaattacc cggctttgtt atggaataag
    60841 gcggtgccta actcgacgtt tcgcccgatg gttgatatag ctacgctgat atcagaagtt
    60901 ggacggcagg caccttgttg tgcgtcattc gaattttatg tagcacgtcc cgaaggggct
    60961 gacataagtc ggtgaatgag ccactgctta ctattatgca gtaactgtgc caataaagaa
    61021 aatagtttgg taacgtactg attatacgtt gctttgaggg atttatggct tcttcgctat
    61081 ttgaatgtaa atcagccact gtgtcgctaa aaagagacaa cttaagataa acttattaga
    61141 ttacaataaa atcaatcatt taagtgtcgc cagaaagcga cacggtaaac cacccattgt
    61201 cgtgatttac agacacaaaa aagctcccgg agttgggagc ttatgatagt ggttggtgct
    61261 taatgccgca tccgggctgc aaaaccaatg aactgatgaa ttaccccaac tgcttacgcg
    61321 cattgcggaa aatgcgcatc catgggccat cctcgcccca gttttccgga tgccaggagt
    61381 tgctgacagt acggaaaaca cgttccgggt gcggcatcat aatggtgact cgaccacttt
    61441 cagtcgtgac tgccgtaata ccgttcggtg aaccgttcgg gttagccggg taggtttcag
    61501 tgactttgcc gaagttatcg acatagcgca gtgccaccag cactttgctt tccagtgccg
    61561 ccagatgcgc cgcatcacgc acttccacgc gcccttcacc atgagagaca gcaatcggca
    61621 tttgcgagcc caccatcccc tgcaacagca gagacgggct ttgggttact tcaaccaggc
    61681 tgaaacgcgc ttcaaagcga tcggaggtat tgcgcacaaa acgtggccac aactcactac
    61741 ctgggatcag ttcgcgcagg ttagacatca tctggcaacc gttacatacc cccagcgcca
    61801 gcgtttgcgg acggtggaag aaggttgcaa actcatcgcg tacacggtca ttgaacagga
    61861 ttgacttcgc ccatccttca ccggcaccca gcacatcacc gtaggagaaa ccaccgcacg
    61921 cgactagggc gtggaaatct tccaggcccg tgcgtccagc cagcaggtcg ctcatatgca
    61981 cgtcgatagc atcaaagcct gcacggtgga aagctgccgc catttcaaca tgcgagttca
    62041 cgccctgctc acgcagtacg gcaactttcg gacgtgcgcc agtggcaata tacggtgctg
    62101 ccacatcttc gttgatatcg aacgacagtt ttacattcag gcccggatcg gcgtcgttag
    62161 atttcgcctg atgctcctga tcggcacact ccgggttgtc acgcaggcgc tgcatctgcc
    62221 aggtagtttc tgcccaccag acacgcaacg tggtgcggct ttcgctgaat acagtctgcc
    62281 cgttggcggt aatcacaaaa cggtcaccgg aaaccgcctg ccctacataa tggacacaat
    62341 cagcaagccc atgctgtgcc agtacggact cgaccgcttc acggtcagcg gcacgaacct
    62401 gaatcaccgc acccagttct tcgttaaaca acgccgccag gcgatcgtcg cccagagagg
    62461 caatatccgc gttaatgccg caatgaccag caaaggccat ttccgccagc gttaccagca
    62521 ggccgccatc agagcggtcg tggtatgcga gcagcttacg ctgtgcaacc agcgcctgaa
    62581 tcgcgtcata gaagcctttc agttgcgcga cgtcgcgtac atctgccggt ttgtcgccaa
    62641 gctgacgata aacctgcgcc agcgccgttg cgcccagcgc gttattgcct ttgcccaaat
    62701 caatcagcag cagtgcgtta tcttcggtag aaagctgtgg cgtgatggtg tgacgtacat
    62761 cttccacgcg ggcaaatgcg gaaatcacca gcgacagcgg cgacgtcatt tcgcgctctt
    62821 cgttaccttc ctgccagcgg gttttcatcg acatagagtc tttacccacc gggatcgtaa
    62881 gacccagcgc cggacaaagc tcttcaccca cggctttaac ggcttcatac aggcccgcat
    62941 cttcaccagg gtggcctgcc gccgccatcc agttggcgga aagtttaatg cgtttgatat
    63001 caccaatctg tgttgcggca atattggtta acgcttcacc gaccgccaga cgggcagagg
    63061 cggcgaaatc cagcagcgca accggcgcac gctcgccaat cgccatcgct tcaccgtagt
    63121 agctgtcgag gctggcggta gtgaccgcgc agttagcgac cggcacctgc cacggcccca
    63181 ccatctgatc gcgcgctacc atgccggtta cgctgcggtc gccaatggtc accaggaagg
    63241 ttttttccgc cacagtcggc agatgcagca cacgtttcac cgcatctgca atggtgatcc
    63301 cttcacgggc cagcgcgtcg cctttcgctt tcagcgtttg tacatcgcgg gtcatcttcg
    63361 gcgttttacc aagcaggacg tccagcggca gatcgatcgg ctgattatca aaatgacgat
    63421 cgtgcagaga aagatgcagt tcttcggtcg cttcaccaat caccgcgtag ggtgcgcgtt
    63481 cacgcttaca cagttcgtca aacagcggta attgatcggc agcaaccgcc agcacgtagc
    63541 gttcctggga ttcgttacac cagatttcca gcgggctcat gcccggttcg tcgcttagaa
    63601 tatcgcgcag ttcaaattta ccgccgcgtc cgccgtcgct caccagctcc ggcatcgcgt
    63661 tagaaagacc gcccgcaccg acgtcgtgga taaacagaat tgggttagca tcaccaagct
    63721 gccagcaacg gtcgatcact tcctggcagc gacgctccat ctccgggttg tcgcgctgta
    63781 cggaagcaaa gtcgaggtcg gcatcagact gacctgacgc catggaagac gccgctccgc
    63841 cgccaagacc gatgttcatt gccgggccgc cgagaacgac cagcttcgca ccgacgttga
    63901 tctcgccttt ttgtacgtga tcggcgcgaa tgttgccgat cccgcccgcc agcatgatcg
    63961 gtttgtgata accgcgcagc tcttcgccgt tgtggctgtt cactttttct tcataagtac
    64021 ggaagtagcc attcagtgcc ggacgaccaa attcgttgtt aaacgccgcg ccgcccagcg
    64081 ggccttcggt catgatgtcc agcgcggtga caatgcgctc aggcttaccg aaatcttctt
    64141 cccacggctg ttcgaagcca ggaattcgca ggttggaaac ggagaaacca accaaacccg
    64201 ctttcggctt cgcgccgcgc ccggtggcac cttcatcgcg gatttcaccg ccggaaccgg
    64261 tcgccgcgcc cggccacgga gaaatcgccg tcgggtggtt gtgagtttcg actttcatca
    64321 gaatatgcgc cggttcctga tggaaatcgt agcggcccgt ttcgtggtca gcaaagtagc
    64381 ggcccacttc agaaccttcc attacggcgg cgttatcttt ataagcagag agaacgtaat
    64441 ctggcgtggt ttcgaaggtg tttttgatca tcttaaacag cgatttcggc tgttgctcac
    64501 cgtcgataac ccaatcggcg ttaaaaatct tgtggcggca gtgctcggag ttcgcctggg
    64561 cgaacatata cagctcgatg tcgttcgggt tacgaccaag ctttgtgaaa gcatcctgca
    64621 gatagtcaat ttcatcttcc gccagtgcca ggccaagacg caggttagcg tcgattagcg
    64681 cctgacggcc ctgccccagc aaatcaacgc tggtgaccgg agtcggttga tggtgtgcaa
    64741 acagctgctc tgcatcatct aaagcaaaaa agaccgtttc catcatgcgg tcgtgcagtt
    64801 cagcggtaac ctgctgccat tgttcattgg tcagcgtacc ggcttctata tagtaagcaa
    64861 cgccgcgctc aaggcggttt acctgttgta gcccgcagtt atgggcaata tcggtcgctt
    64921 tcgaagacca gggagagatg gtgccaggac gcggggtcac cagcaggagt ttgccttgcg
    64981 gggcgtggct ggcgagtgcc gggccatatt tcagcaggcg ttcaagttgt gcgtgctcat
    65041 catcgtttaa cggcgcattg aggtcagcaa aatggacata ctcggcgtaa atattgtgaa
    65101 ccgggagcct ggcagcctga aaacgtgcca gcagtttgtt gattcggaat gccgacagtg
    65161 caggcgaacc acgcagaatt tccatcataa gtctctcgtc ttctaagctt tcggtgtacc
    65221 caaggggggg aaacgggcgt cattataaag aatctgatgc gctgacgaaa ccgtttgcgt
    65281 ggaaataaaa tcaccatcgt gaattagcaa cgcgtgccgc caatggctgt aataagttgc
    65341 catctggcgc aggtttacgc aaaatgccgc tcatttatga gtaaaccttt cactattatt
    65401 acgttttttc aagctgggac gcgcacgaca cagagaatta actaattgaa aaaattaaag
    65461 attaattatc tgttcatcgg cattctggca ctgctgctcg cggtcgctct ctggccatcc
    65521 attccctggt ttggtaaagc cgacaaccgt atcgccgcca ttcaagcgcg gggagagttg
    65581 cgtgtgagca ccattcatac tcccctgact tataacgaaa tcaacgggaa accttttggc
    65641 ctggattacg aactggcgaa acagtttgcc gattacctcg gcgtaaaact gaaagtgacc
    65701 gtgcggcaga atatcagcca gctgtttgac gaccttgata atggtaacgc cgacctgctg
    65761 gcggcaggac tggtctataa cagtgagcgg gtaaaaaatt atcagcctgg ccctacctat
    65821 tattccgtgt cacaacaact ggtttataaa gtgggtcagt atcgcccacg tacgctgggc
    65881 aacctgacgg cggagcaact caccgttgca ccgggtcatg tggtggttaa cgatctccag
    65941 accctgaaag aaacaaaatt cccggaatta agctggaagg tagacgacaa aaaaggctct
    66001 gcggaattaa tggaagatgt catcgaagga aaactcgatt acaccattgc tgattctgtc
    66061 gccatcagcc tgtttcagcg cgttcacccg gagctcgccg tagcgctcga tatcaccgat
    66121 gaacaaccgg tgacttggtt tagcccgtta gatggcgata ataccctttc cgccgccctg
    66181 ctcgacttct tcaacgaaat gaatgaagac ggtacgctgg cacgcattga agagaaatac
    66241 ctggggcatg gcgatgattt tgattacgtc gatacgcgca catttttacg cgccgtcgat
    66301 gcggtactgc cgcagttaaa gcccctgttt gagaaatacg ccgaagaaat tgactggcgt
    66361 ttgctggccg ctattgctta tcaggaatcg cactgggatg cacaggccac ttcaccgacg
    66421 ggtgtgcgcg gcatgatgat gttaaccaaa aataccgcgc aaagcctcgg cattacggat
    66481 cgtaccgatg ccgaacagag catcagcggt ggcgtgcgtt atttgcagga tatgatgagt
    66541 aaagtgccgg aaagtgtgcc ggagaacgag cggatctggt ttgccctcgc tgcgtacaat
    66601 atgggctatg cgcatatgct ggatgcccgc gccctgacgg caaaaaccaa agggaatcct
    66661 gacagttggg ctgacgtaaa acagcgtctg cctttactta gccagaaacc ctattacagc
    66721 aagctgactt acggctacgc tcgtggacat gaagcctacg cttatgtcga aaatattcgt
    66781 aagtatcaga ttagcctggt gggttatctg caagagaaag agaagcaggc tacagaagcg
    66841 gcgatgcaac tggcgcagga ttatccggcg gtatcgccta cggagttggg caaagagaaa
    66901 tttccttttc tctcgtttct ttcccagtcg tcatcaaact atttgaccca ttctccctct
    66961 ctgctgtttt ccaggaaagg gagtgaagag aaacaaaatt aatccgtcga ggattgcgct
    67021 tttttctgcg ctttaatttc ctggcggcgc atgcgaaaga agtcactgag caacgccgcg
    67081 cactcatccg ccagtattcc ttccgtaatt tccactcggt gattcatacc cggatgatgc
    67141 agcacatcca ttaaagatcc cgcagcgcca gttttcgcgt cacgcgcacc aaagaccacg
    67201 cgaccaatgc gactgtggat catcgctccg gcacacatta cacatggttc aagcgtgaca
    67261 tacaacgtgg cgtcgatcag acgataattt tgcatcacca gaccaccctg ccgcagggcc
    67321 atgatttctg catgtgcggt gggatcatgg cgaccaatcg ggcggttcca gccttcgccg
    67381 attacccgat tgttatgcac taataccgcg ccgaccggca cttcccgctc atcccaggca
    67441 cgtttcgcca gcgtcagcgc gtgacgcatc cagtattcgt ggctaaattc gacttcagac
    67501 aaaaagaaaa ctccggttat aaaagcgcgg cgcattatac acggacgcta tgctttactc
    67561 tattccagtt gctggagttc accgcgcggg gttactcgcc aacgatgctg acagaaataa
    67621 agcagcggat tgtcctgatt actgtcgcta tagccactgt acagccgcag cggagtgccg
    67681 attttgcgct ccagttgtgc gaccttttca tgtcccagac aacgcatcgt caatacccaa
    67741 ccaccatagc cacgctgaat ttggctggcg ataagattaa cccgcggcag ccagggcgta
    67801 tcgaaataaa ccgcttcaac cagcggctgc ggagagccgg taatcaacca gatatcagca
    67861 tcggaactta acaggtaggt ggttaatcgc tcctgaacca gcggaaaggc ggtaacattg
    67921 tcgcgaaacc agcgcacgaa atcggcctgc aacgtctgta aacgtgcttc gctgtgacca
    67981 aaagtgcacc cccacagaag cagactcatc ggccagcgtg ccgcacgacc ttttatcaat
    68041 aacgcaatgg ctataatcgg taacaacggc aggacaagta acgcattcag cggttggcga
    68101 cgcagtaaat agcgcagaaa actgccgaac atatcctgct gatgcaatgt tccatctaag
    68161 tcaaaaaaca ccacacgacg ctcgtgagtt gccaaaccat actcctctgg actgaaacat
    68221 cctgattaat tttcgctact acatagccta acagatagat catcactttt ccggcaacaa
    68281 tcgaccgcat catggctaac tggaattttt aattcatggc aattagcggc aatggaatat
    68341 aaaattcact cgcgtgtctc tcatattgcc tgttgtcgcc actatttcat cgcgaggaaa
    68401 gatatgaacg gcttacttcg tatccgtcag cgttaccagg ggcttgccca gagtgataaa
    68461 aaactggcgg attatctgct gctacaacct gatacggcgc gccatttaag ctctcagcaa
    68521 ctggccaacg aagccggagt cagtcagtcc agcgtcgtga agttcgcgca aaaactcggc
    68581 tataaaggtt ttccggcgct taagttggcg ttgagtgaag cgctggcaag ccagccggaa
    68641 tcgccctccg tgcccattca taaccaaatc cgcggtgatg atccgttacg gctggtcggc
    68701 gaaaaactga ttaaagaaaa tactgccgct atgtacgcaa cgctaaacgt taatagtgaa
    68761 gagaaactgc atgaatgcgt aacaatgttg cgctctgcgc ggcggataat tctgaccggt
    68821 attggcgctt cgggtctggt ggcgcaaaac tttgcctgga agctgatgaa gattggcttc
    68881 aatgctgccg cagtgcgcga tatgcatgcg ctactcgcaa cagtacaggc gtcgtcccct
    68941 gacgatctgt tattagccat ttcctacacc ggtgtacgac gcgagttaaa cctggcggca
    69001 gatgagatgc tgcgagtggg cggaaaagtg ctggcgatta ccggctttac tccgaatgcc
    69061 ctgcaacagc gtgcttctca ttgcctgtat accattgccg aagaacaagc gacaaacagc
    69121 gcttcaatct ctgcttgtca cgctcaggga atgttaacgg atttgctgtt cattgcgctg
    69181 attcagcagg atctggaact ggcaccagaa cgtattcgtc atagtgaagc gctggtgaaa
    69241 aaactggtct gagtaaagaa tgcgcgtata atgcccgccc ggtttgtgtt gttttgagag
    69301 tttccttatg gcgttgttaa tcactaaaaa atgcatcaat tgtgatatgt gtgaacccga
    69361 atgcccgaat gaggcgattt caatgggaga tcatatctac gagattaaca gcgataagtg
    69421 taccgaatgc gtagggcact acgagacacc aacctgccag aaggtgtgcc cgatccccaa
    69481 tactattgtg aaagatccgg cgcatgtcga gacagaagaa cagttgtggg ataaatttgt
    69541 gctgatgcac cacgcggata aaatttaata ttctactctg gaagtagagt attaattata
    69601 ttactgggaa gccttaacgc cattatattt atttaattga tgacattagc ataatcattc
    69661 actaagttaa tttatatagt atctgccaag acacttattt atagttatta aaggcgcgtc
    69721 cgattggttc accggacgca ccttaagtac gtttccttgt gttataagaa cagaaggatc
    69781 agctgtaaaa cagcaatgat gattttgatg acccgtttaa tcaggtatcg gcaatcagtc
    69841 attcgttttt ccttaaacaa ggaatgcagc cgttcagatt agcccttaca tctccccaaa
    69901 actgaacgtg cgagttattg agggtgcatg ctgcactcca caccagagct ttgacgacac
    69961 cactcgtttc aatgggggaa ttctgtggca tggtgtaaag cacagcaaaa tcttcaataa
    70021 cgaagccaat tttaatgtac ttacgaattg gcggtcacgt taatctttcc atcaatattg
    70081 ctttcttcgt aaaggctcga gtttttatgc taaagattgc aagttgcttg taaaagataa
    70141 gtacactgat ccataatcgc tgttgttgag ggtgcatgct gcacaaaatt aaagttaaaa
    70201 agtaaaaccc ccgttcctta ccagttcggg ggttttactt tttaaagaga acggtattat
    70261 tttaactttc aataattacc gtggcacaag catagtggcg ttcatctgcc agcgttacat
    70321 gcatatttac aacgccgagc ttttccgcca gttttaatgc ctcaccccat agccgtagcc
    70381 gtggtttgcc gagctcatca ttgaatactt caaattgatt aaacgccaga ccattgcgga
    70441 tcccggtgcc aaacgctttt gctgcggctt ctttcacagc aaaacgcttc gccagaaaac
    70501 gcaccggctg gtggtgcgtt ttccagattg cccattcgtt atcgcttaat acgcggcgtg
    70561 ccaggcgctc accggatcgg gcgatcactg cttctatgcg agcgatctct acgatatccg
    70621 tgcctaaacc taatattgcc attagccacg cgcttccagc atcagacgct tcatttctgc
    70681 caccgcatct ttcagtccgg tcatcactgc acgaccaata atggcatgac cgatattcag
    70741 ttcatgcatc tcagggatgg cggcaatagc tttcacgttg tgataggtca gaccgtgacc
    70801 agcgttaact ttcagaccga gactagcggc aaaggtcgcg gctttggcga tacgcgccag
    70861 ctcttgcgcc tgttcggcgt cagttttggc atcagcatag caaccggtgt ggatctcgat
    70921 aaacggtgcg ccaacctctg ccgcagcttt gatctgctct tcatcggcgt cgataaagag
    70981 agaaacctgg atcccggcat ctgccagacg tttgcaggca tcgcgcattt tgtcacgctg
    71041 ccctgcgacg tccaggccgc cttcggttgt tacttcctga cgcttttccg gtaccagaca
    71101 gcaaaaatgt ggcttcgtct caacggcgat cgccagcatc tcttcggtca ccgccatctc
    71161 cagattcatg cgggtatcca gcgtctgacg caggatgcgc acatcacggt cagtaatgtg
    71221 gcggcgatct tcacgtaaat gcacggtaat gccgtccgct cccgcctgct cagcaataaa
    71281 cgccgcctgc accggatccg ggtaagcagt accgcgtgcg ttacgcagcg ttgcaatatg
    71341 gtcaatgttg acgcctaaca gtaattcagc catgacaatc ctcatcattc ataatgtgtt
    71401 ttcaccgttc gcttaggcat aaactgccgg aacagttccc tgctctttaa tggtttaccg
    71461 ccaagatacg gcttaagcgc catgcgggta aagcgtttcg cggcgcgtag tgtgtctgcg
    71521 tcaggaaatt cccgcgcgtt taacgctttt aactgccttc cggtgaacgt tttattgtcg
    71581 ataacgacgc ttgcgataaa ccctttttct tcgcgataac gatacgtcat ggtgtcatct
    71641 accggctcgc cgctacccgc acaatgggta aaattgacgc catagcccag atgcccgagc
    71701 agtgccaatt caaagcggcg cagcgcgggt tctggcgtac cagtggcacc tgcaagagac
    71761 tgaatgcagt gcaagtaatc gaaaaagagt tcagagaagc gcgtctcgta ttccagtacg
    71821 cgggagagaa gttcgttgat gtacagaccg ctgtaaagcg tgataccgct taatggcagc
    71881 gccagcgaga cagcttcagc actgcgcagc gttttgactt cgccacgccc gccgaaacgt
    71941 agcaagagag gggtgaaagg ctgtaatgca cctttcagag tagagcgttt agagcgtgcg
    72001 cctttggcaa ccagacgcac gcgacccgat tcctccgtga agacgtccag catcaggctg
    72061 gtttcgctcc acgggcgact atgcaggaca aatgcgcgct gccagccttc catcggagtg
    72121 actcttaaag atcgtcaacg taaccgagac tgcgcagtgc gcgttcgtcg tcggcccaac
    72181 cggatttcac ttttacccac agttcaaggt ggacaggcgc ttcgaacatt tcctgcatgt
    72241 ctttacgcgc ttcaatcccg atggttttga tcttggcccc tttgttgcca ataaccatct
    72301 tcttctgccc ttcacgctca acgagaatca aaccattgat gtcataacca ccgcgttcgt
    72361 tagagacgaa acgttcgatc tccacggtca cggagtacgg cagttcagcg ccgaggaaac
    72421 gcatcagttt ttcgcggatg atttcagacg ccataaaacg ctgtgagcga tcggtgatgt
    72481 aatcttccgg gaagtgatgg gtcgcttcag ggaggtgctt acgcacgatt gctgcgatag
    72541 tgtcgacatt cagcccggtt tcagcagaga tcggcacgat atcgaggaag ttcatctgac
    72601 ttgccaggaa ctgtaagtgc ggcagcagat cggctttttc ctgcacgtta tccactttgt
    72661 tcaccgcgag gatcaccggc gctttgccat cgcgcagttt gttgagcacc atttcgtcgt
    72721 ccggcgtcca gcgggtgcct tcaacgacaa aaatcaccag ctcaacgtcg ccaatagagc
    72781 tgctcgccgc tttgttcatc aggcggttaa tagcgcgttt ttcttccata tgcaggccag
    72841 gggtatcaac gtagatcgcc tgatacgcgc cttcagtatg gatccccaca atgcggtgac
    72901 gagttgtttg cgctttgcgg gaggtgatgg agatcttctg ccccagcagt ttgttcaaca
    72961 atgtggattt gccaacgttc ggacgtccga cgatggcaat aaatccgcag taacttttat
    73021 cgatgctcat tccagctcca gttttttcaa cgcctgttcg gcggcagcct gctcagcctt
    73081 acgacggctt gaacctgtgc caaccaccgg ttcactcagg ccgctgacct ggcagtggat
    73141 agtaaattcc tgatcgtgcg cttcgccacg tacctggact accagataag tcggcagcgg
    73201 cagatggcga ccctgcaaat attcttgcaa gcgcgttttc ggatcttttt gtttatcgcc
    73261 tgggctaatt tcgtccaaac gagtttgata ccagttgagg attaatttct cgacggtttg
    73321 aatatcactg tcgaggaata cgccaccaat taatgcttcg acggtgtcgg cgagaattga
    73381 ctcacgacga aatccaccgc ttttaagttc acctggccct aaacgtaagc actcgcctaa
    73441 ctcaaattcg cgcgccagtt ccgccagcgt attgccacgg accagcgtgg cgcgcatccg
    73501 gctcatatcg ccttcatcca cacgagggaa acggtgataa agcgcattgg cgataacgta
    73561 gctcagaata gagtcgccta aaaattctaa acgctcgtta tgtttactgc tggcactacg
    73621 atgagttaat gcctgctgca acagttcctg atgattaaaa gtgtagccca gcttccgttg
    73681 aagccgatta attacgatgg ggttcatgcg ataccaataa ataaatgcgt caacaattca
    73741 gcacacgaaa cagacctgat atacatggct ctgctaactg cttcgctgca gtttcagttt
    73801 accggtatat ggggaccaac gctgtttcgt gtgccgtggc aacctggagg tgccaacctt
    73861 aaacttcggg ggaatattct atacacaacg acgggggatg tcgttagcca cgggagattt
    73921 atctcataaa taattcacgt tgtcgccata acggcgacaa cgtgaacgaa gatgagctat
    73981 taatggatgc cgccaatgcg acttaagcgc acaccagtcg gccattcgcc ttcttgctta
    74041 tcgaagctca tccagatagc cgttgcccga ccgaccagat tcgcttcagg cacaaacccc
    74101 caataacggc tgtccgcgct gttgtcgcgg ttgtcgccca tcatgaagta ttgtcccgga
    74161 ggaacaatcc aggttgccag ttgttgccct ggctgctggt aatacatccc cacctgatcc
    74221 tgcgcaatcg gcactgtcag aattcggtgc gtcacatcac ccagtgtctc tttacgctcg
    74281 gaaagacgaa ttccattttc tttggtttcg tttttcggca cttcaaagaa tccgctggtc
    74341 gcttccccac cattacggcg tgagaaggtc tgaacgaaat cgctcggttc cacgtttgag
    74401 taggtgaccg gcagcgcgtt ttcacacgcc tggccggaac tgcatcccgg ttgaatcgtc
    74461 agctctttcg agaccggatc ataagtaact ttatcgcccg gtaaacccac cgcgcgcttg
    74521 atgtaatcaa gctttggatc ttccggatat ttaaagacca cgatatctcc gcgtttcgga
    74581 tgaccggttt cgatcagcgt tttctggtag ataggatctt taatgccata agcaaacttt
    74641 tctaccagaa taaaatcacc gattaacaga gtcggcatca tcgaacctga cgggatctgg
    74701 aacggttcat aaataaacga acgcacaatc aatacgatag ccagtaccgg aaaaacagaa
    74761 gcaccggttt ccagccagcc aggcttcggc gcaacctttt tcaacgttgc tttatccagt
    74821 gagtcaccgg cagccgcctg cgccgctgcc tgacgttccc gccgtttagg tgcgaaaaag
    74881 aatttatcca cgcaccataa aatgcccgtc accagtgtgg caatcaccag aatcagggca
    74941 aacatattcg ccatgccaac tcctaagggt tatttgttgt ctttgccgac gtgcagaatg
    75001 gcgaggaacg cttcctgcgg cagctcgacg ttaccgatct gcttcatgcg tttcttacct
    75061 tctttctgct tctgcagcag ctttttctta cggctgatat cgccgccata acatttagcc
    75121 agtacgtttt tacgcagctg tttcacggtg gatcgcgcaa taatgtgcgt accaatcgct
    75181 gcctgaatgg cgatatcaaa ctgctggcgt gggatcagat ctttcatctt ctccaccaac
    75241 tcgcgaccgc ggttttgcga attatcacgg tgggtgatca gcgccaacgc atcaacacgt
    75301 tcaccgttga ttaatacgtc tacacgtacc atgtcggacg cctggaagcg tttgaagttg
    75361 taatccagag acgcataacc acgcgaggta gatttcaggc gatcgaagaa gtcgagcacc
    75421 acttccgcca tcgggatctc gtacgtcagc gccacctgat taccgtggta aaccatattg
    75481 gtctgcacgc cgcgtttttc tacgcacagc gtaataacgt tgccgagata tgcctgcggc
    75541 agcagcatgt gacattcggc aatcggctcg cgcagttcgt agatgttatt taccgctggc
    75601 agcttggatg gactgtcgac gtaaataact tctcttgacg tggtttcaac ttcatacact
    75661 acggtcggcg cagtggtgat cagatccaga tcgtattcac gttccagacg ttcctggatg
    75721 atctccatgt gcagcaggcc gaggaagccg cagcggaaac caaagcccag cgcgctggag
    75781 ctttccggct catagaacag tgaagcatcg ttcaggctga gtttacccag cgcgtcacgg
    75841 aaggcttcat agtcgtcgga acttaccggg aacagaccgg cgtatacctg cggtttgact
    75901 ttcttaaagc caggcagcgc cttttctgcc ggattacgcg ccagcgttaa ggtatcgccg
    75961 actggagcgc cgtggatatc tttaatcgca cataccagcc agcctacttc gccacatttc
    76021 agttcagtgc ggtcaacctg tttcggcgtg aagatgccca gacggtcggc gttataggtc
    76081 tgcccggtac tcatgacttt cactttgtcg cccttacgca gggtgccgtt tttaatacgg
    76141 ataagtgaaa caacgcccag gtagttgtcg aaccatgagt cgataattag tgcctgcaac
    76201 gggccttccg gatcgccttc cggcggcgga atgtcgcgca ccagacgttc gagaacgtcc
    76261 tgcacgccaa cgccggtttt cgctgaacag cgcaccgcgt cggtggcgtc gatgccgacg
    76321 atatcttcaa tttcttccgc cacgcgttca ggatcggctg ccggcaggtc aatcttgttc
    76381 agtaccggca ccacttcgag atccatttcc atggcggtgt agcagtttgc cagggtttgc
    76441 gcttctacgc cctgcccggc gtcgaccacc agcaatgcac cttcacaggc agccagcgaa
    76501 cgggaaactt cataggagaa gtcaacgtgg cccggggtgt cgataaagtt cagctgatag
    76561 gtttcgccgt cagacgcttt gtagtccagc gtcacgcttt gcgctttgat ggttatgcca
    76621 cgctcacgct caagatccat ggaatcgaga acctgcgcct ccatttcacg gtcagacagg
    76681 ccaccgcaga tctggataat acggtcagac agcgtcgatt taccgtggtc aatgtgagct
    76741 atgatcgaaa agttacgtat attcttcata aagtatgatt attgtgcctt aatgcccggt
    76801 taaccaggct tttagaagtc gctgttctga gcttaacgtc tgtattaata gaaacgccgc
    76861 attctacact acaacattga ggcgaggaaa tgttcatacc gtatggattg tggtatctgg
    76921 aaacgtcctc gcatttgtta tgcaaaatgc aacaaagcca gtgaaatcac tggctcgcgt
    76981 cttccgaaga tgtttcaaat cgcacaaggc caggcggcaa tgccacgctt aagatgatcg
    77041 gttgccattc tgcccgggca gcaaacttac gcgagtagcc gcgcgcaatc aggaacccgc
    77101 caataccgcc gaggaccgca ccacataatg ctgcgacgtc agaagcaaag agtagctgaa
    77161 atagcgaagc gatgaggaat aatcccacca gcggcgacat ataaaccagt aatgcggagc
    77221 taagcaggct gccttcggcg atccccaatt ccactttttg ccccggcact aacggctcat
    77281 cacagggtac gacaatggta tgcgtggttt gcgggccaag tttatttaac acgcggctac
    77341 cgcaaccggc gcgtgaagcg cagctgctgc atgaagcttt aacatcacaa ctgaccagcg
    77401 cctgcccgtt ttgccaggag acgacggtag cccactcttt gatcattgcg ctgccccgaa
    77461 cttaatattc tcggcaatgc gtttcgccgt ttgcggcggc agttcaccga caatggtgat
    77521 ttcggcgtta tcacgtacgc ttgtactgac ggttctgcgt ccggtgcgca acatctgatc
    77581 ggtgctcgat ggcgtagcgc ggttaacgtt taccgagaag ctgaataatc cgtcggaata
    77641 gagacgtgat tcgataggca tgttgtccat cgtcggtagc ggacgtcgac tactggaaac
    77701 ttcgctaaaa ccctgtggca accaggttgg cgtccagctg aatttagctt tttcacctac
    77761 aggaacagaa agcaacggcg gcaaatttgc cttcgccagc gtctgcatac tgctgctgat
    77821 atcctgattg acgttaaaag caatcacgcg aaattgttcc agcgtttcac catcgcgatc
    77881 aaggagatca acccgcatcg gtaatttcga ttcggtgtcc atccacacga tgtagctgta
    77941 gcgtgtacca tctcgggcaa ccacgcgaat gacttcgcaa agacgatcag caatacgcgt
    78001 gcgcccgacg gagataaagt cgtagtaagg agaaaggcgt ttgaaatcgg tatagataag
    78061 cgatggcaga gaatcaacaa tgtaatcgcc attaagcgtg aacggttcaa gtcccggttc
    78121 aaaatagctg atttcattgc cgcgctgtac cacttcccgg cgcgggccat ccatttgcaa
    78181 caattgtgca agaggacggt tatcgaggcg tgcatgtcga taacgcagag actcaacacc
    78241 ctgtttattg atgctgatga atgacagctc gtaattcagt gactgactgg ccaggttcat
    78301 ctgctgtaat aacgccccgg acgcgggagt ggccgaggcg ttagcagaga ataacaggct
    78361 acctgtcact aatgacatgg caaaccaaag ttgcttcatt actgcgattg cgttcctaaa
    78421 gtttgaattc ctggcacctg tacagcggct tgctgggttt gcgcctgctc aaactgaagc
    78481 tgttcagagt ggagtcggcg ttgcagttcg taatcctgca acattgcatt aatgcgacga
    78541 cgctgctcct gtacctgctg ctgttgacca ttgtttgcgg tcgcttcaga aggtactccc
    78601 aggcttaccg ggctggcttt acccatcatc ggcagtgtat taaataccgg cgtttcgggc
    78661 tgctgggacg tttcagattg tccattatag tgctggacgc caacgataac tgcaagcgat
    78721 acgcatgctg ctacgcccat ttgggtaagc tgtgccgccc acggacgtac tttctgccag
    78781 aatggcattt tctgccattg atgcggcgca ggctgggctt ccgggatcaa tgtcgccggt
    78841 tgacgtactg gctcttcttc aatggcggcc atcacgcgtg aagagatatc gaaatggagc
    78901 acctcgggag tatcaccccg cattgagtca cggattaagt gatagctttc ccaggttttc
    78961 tgcatttctg ggttatgagc cagttcgtta agcagctcac tatccagcgt ttcgccatcc
    79021 attaaagcgg aaagttgttc tttctgcatg cctaataccc ttatccagta tcccgctatc
    79081 gtcaacgcct gataagcggt tgaactttgt tatcaatagc ttccctcgct cggaagatac
    79141 gtgaacgcac cgtacctacc ggacaatcca tgatagcggc tatctcttca tagctcaggc
    79201 catccagctc ccgcaaggtt attgccatgc gcaaatcttc cgggagggac tcaatagttc
    79261 ggaaaactat ctgtctcagt tcttctgaca acattaagtt ctcagggttc gaaatttctt
    79321 tcaacgcgcc gccactttcg aagttttcag cttcaatggc atccacatca ctggaaggtg
    79381 gacgacgccc ctgagcaacc aggtaatttt tcgctgtatt tacagcaatc cgatacagcc
    79441 atgtataaaa agcgctatct ccccggaacg aatccagcgc acgataggct ttaataaaag
    79501 cttcttgtac cacatcggga acatcacccg acggcacata gcgggaaacc agactcgcca
    79561 ctttatgctg atagcgcact accagtaggt taaaggcttt ctgatctccc ttctggaccc
    79621 gttcaaccag gacctggtcc gttaactgct cgctcatccg aggtaaagtc tccccaaacc
    79681 aaatttccac gcgctatcga aacgccactc cattagctgc aatttgagca agcaaagggt
    79741 tagagtgtct cgtttttgta aagttccgta acgcatctgt ttttgtttgt catgctgtag
    79801 acggatcatt atctatcatt ataagtctac agaatctgaa catcgcatta tctgtgtaga
    79861 aatgcccatt taactgcctg aagagtaacc caacggcctt tttatttcac cacctaatcc
    79921 tccaccagcc agtaacttct ctttttctcg ccgccctgcg tcagcgtgtt tagccactgt
    79981 aacaaatatt aaaatagcag gtgtttattc gcacaacatg atgctatgct gaccaaacag
    80041 tgtttagtaa attaaacaaa gaaaatgaat actctcccag aacattcatg tgacgtgttg
    80101 attatcggta gcggcgcagc cggactttca ctggcgctac gcctggctga ccagcatcag
    80161 gtcatcgttc taagtaaagg cccggtaacg gaaggttcaa cattttatgc ccagggcggt
    80221 attgccgccg tgtttgatga aactgacagc attgactcgc atgtggaaga cacattgatt
    80281 gccggggctg gtatttgcga tcgccatgca gttgaatttg tcgccagcaa tgcacgatcc
    80341 tgtgtgcaat ggttaattga ccaaggggtt ttgtttgata cccacgttca accgaatggc
    80401 gaagaaagct accatctgac ccgtgaaggt ggacatagtc accgtcgtat tattcatgcc
    80461 gccgacgcca ccggtagaga agtacaatcc acgctggtga gcaaggcgca gaaccatccg
    80521 aatattcgtg tgctggagcg cagcaacgca gttgatctga ttgtttctga caaaattggc
    80581 ctcccgggca cgcgacgggt tgttggcgcg tgggtatgga accgaaataa agaaacggtg
    80641 gaaacctgcc acgcaaaagc agtggtactg gcaaccggcg gtgcgtcgaa ggtttatcag
    80701 tacaccacca atccggatat ttcttctggc gatggcattg ctatggcgtg gcgcgcaggc
    80761 tgtcgggttg ccaatctcga atttaatcag ttccacccta ccgcactgta tcacccacag
    80821 gcacgcaatt tcctgttaac ggaagcactg cgcggcgaag gcgcttatct caagcgcccg
    80881 gatggcacgc gttttatgcc cgattttgat gagcgcggcg aactggcccc gcgcgatatt
    80941 gtcgcccgcg ccattgacca tgaaatgaaa cgcctcggcg cagattgtat gttcctcgac
    81001 atcagccata agcccgccga ttttattcgc cagcatttcc cgatgattta tgaaaaattg
    81061 ctcgggctgg ggattgatct cacacaagaa ccggtaccga ttgtgcctgc tgcacattat
    81121 acctgcggtg gtgtaatggt tgatgatcat gggcgtacgg acgtcgaggg cttgtatgcc
    81181 attggcgagg tgagttatac cggcttacac ggcgctaacc gcatggcctc gaattcattg
    81241 ctggagtgtc tggtctatgg ctggtcggcg gcagaagata tcaccagacg tatgcctgat
    81301 gcccacggcg tcagtacgtt accgccgtgg gatgaaagcc gcgttgagaa ccctgacgaa
    81361 cgggtagtaa ttcagcataa ctggcacgag ctacggctgt ttatgtggga ttacgttggc
    81421 attgtgcgca caacgaagcg cctggaacgc gccctgcggc ggataaccat gctccaacaa
    81481 gaaatagacg aatattacgc ccatttccgc gtctcaaata atttgctgga gctgcgtaat
    81541 ctggtacagg ttgccgagtt gattgttcgc tgcgcgatga tgcgcaaaga aagccggggg
    81601 ctgcatttta cgctggatta cccggaactg ctcacgcatt ccggtccatc gatcctttcc
    81661 cccggcaatc attacataaa cagataaaaa gcctgggtca gcgccgtata cgcttcggaa
    81721 tagttctggt ctggcccacg aatgactaag cgatcgctaa agcattctcc cgcctgcggg
    81781 gagaatgcca gcagcacccg atgcggcagt cgcgcttcgt tttccgccac atccgtccgc
    81841 aaacgtaaat gccagcccat gcttaatgcc agttcagtaa aaccattacc aatctgctct
    81901 ggcagcacca cgcagaaaaa tccctcttcg gtaatgcact ccgccgcaca ggtcagcagc
    81961 gatgggtgat caagcgtagt ggtatagcga gcctgttccc gttgaggtgt cgcgcactct
    82021 actccctgct gatagtaagg tgggttactg atgattaaat cgaagcgtac tgtctgctgt
    82081 gtgatccact gctgaatatc cgccgtatgg acgttaatcc gctctgccca cggggactgg
    82141 ttgatatttt cctgcgcctg cgcggcagct tcactttcca gctcaacggc atcaattatc
    82201 acgctgtcac tggttcgctg cgccagcatc agtgccagca acccgctacc cgcgccgata
    82261 tcaaggcaac gtttcacccc agccaccggt gcccatgcgc ccagcaaaat gccatccgtt
    82321 cccactttca tcgcacagcg atcgtgagca acaaaaaact gtttaaaagt aaatccatta
    82381 cgacgaagca cggatgtaga ctgtgacatg aaaataaaac cttgcaggaa aaacggcgat
    82441 agcaccgggt gagaacaata cccgagaagc gatatccata caaacagatg aagattgcag
    82501 ccgtaacgtc tataatcagc gccccacaca gaggtagaac atgactgtaa cgactttttc
    82561 cgaacttgaa ctcgacgaaa gcctgctgga agccctccag gataaaggtt tcactcgccc
    82621 gaccgccatt caggctgccg ccattccgcc tgcgctcgat ggccgtgatg tactcggttc
    82681 tgcgccgaca ggcaccggta aaacggcggc gtatctgctg ccagcgttgc agcacctgct
    82741 cgatttcccg cgtaagaaat ccggtccgcc gcgtattttg atcctcaccc caactcgcga
    82801 gctggcgatg caggtggccg atcatgcccg cgaactggcg aaacatacgc atctggatat
    82861 cgccaccatc accggcggcg tagcctatat gaaccacgcg gaagtgttca gcgaaaatca
    82921 ggacatcgtg gtcgccacga ccggacgtct gctgcaatac ataaaagaag agaacttcga
    82981 ttgccgcgcg gttgaaacgc tgatcctcga cgaagcagac cgtatgctgg atatgggctt
    83041 cgctcaggat atcgaacata ttgctggcga aacgcgctgg cgtaaacaga ccctgctctt
    83101 ttcggcaacg ctggaaggcg atgcgattca ggactttgcc gagcgtctgc tggaagatcc
    83161 ggtggaggtt tctgccaatc cgtccacccg tgagcgcaaa aaaattcatc agtggtatta
    83221 ccgcgccgat gacctggaac ataaaaccgc attgctggtg catctgttaa aacagccgga
    83281 agcgacccgc tcaattgtgt ttgtgcgtaa gcgtgaacgt gtgcatgagc tggcaaactg
    83341 gctgcgcgaa gcgggcatca acaactgcta tctcgaaggt gagatggtac aaggcaagcg
    83401 taacgaagcg atcaagcgtt tgaccgaagg tcgcgtaaac gtactggttg caaccgatgt
    83461 tgccgcgcgc ggtatcgaca ttcctgacgt cagccacgtc tttaacttcg atatgccgcg
    83521 cagtggcgat acttatttgc accgtatcgg acgtaccgcg cgcgccggtc gtaaaggcac
    83581 cgcaatttcg ctggtggaag cccatgacca tttgctgctg ggcaaggttg gccgttacat
    83641 tgaagagccg attaaagctc gcgttattga tgagttacgc ccgaaaacgc gtgcgccaag
    83701 cgagaagcag accggcaagc catcgaagaa agtactggca aaacgcgccg agaagaaaaa
    83761 agctaaagag aaagagaaac cgcgtgtgaa aaaacgccat cgcgacacca aaaatattgg
    83821 taagcgccgt aaaccaagcg gaacgggcgt gccaccgcaa acgacagaag agtaatctca
    83881 atgccgggtt taagcctggt attaaaagtg ctggcaaacg caaaactgcc tgatgcgcta
    83941 cgcttatcag gcctacgtgg ctcatgcaat atattgaatt tgcacgatct tgtaggccgg
    84001 ataaggcgtt cacgccgcat ccggcatgaa taaagcgcac tttgtcgaca agttaaatgc
    84061 cgggtttaat cccgacatta tttttatcca gccacaaaac tctcttcaac acactgaata
    84121 aaagtgtgca tcgccgggct aaccgctttt ccagcatggt gtgcgcacat ggccgtaatg
    84181 gtctgcgact gttcgccaaa aggcaattca attaattctc cgctctcaag ctctttttcc
    84241 accgcaaagc ggggcaagta actgacaccg atattcgccg cgacacaacg tttgatactt
    84301 tcaatgctta acagctcaat ggtgttttcc accgtgatcc gccgctgacg cagcgtgctc
    84361 tcaaatatct gccggaagac acattgcggt tcgttgataa taaagctaca ggcgttatgt
    84421 cttcccggct cagtaaaatc gacatctgca atttgcggtg aagccaccag caccagcgat
    84481 tgttcaccca actctcgtcg gttcagggca tcatcattcc ctacgcgata aaagacgccg
    84541 acatcggcct catcattcag tagcgcatca cggatcacgt aacagttcag cgactgcaac
    84601 gataaacgca cttttggtgc gcgttgccga aaacgctgca acacctgcgg cattcggtaa
    84661 gagagcaacg tttcgcccga aacaacgcgt agttccccgt ccggatctga ctcctttttg
    84721 gcagcctcac gaagcgtatc catcactcgg gtaagttcat aaatgtgcgg cagcaacttt
    84781 tttccttcgc gggtaaggca catccgtcgg ccaattttct caaataactg gactgaaaac
    84841 tcctgctcaa gctgctgaat atgaaaagtc accgtcgatt gtgtacagca caatttttgc
    84901 gaagctcgca aaaaggaacc ctcttccacc acggttttaa gcgtaataaa acggcgcaga
    84961 tccataaccc caaacctatc gaaaatatcg aatctagaat ataaaaacat tcattttttt
    85021 aaatgttccg tgtcgggtac tgtctaccaa aacagaggag ataacaagtg acaccgcccc
    85081 ttttaagtgc tttttggact tacaccctga ttaccgctat gacgccagga ccgaacaata
    85141 ttctcgccct tagctctgct acgtcgcatg gatttcgtca aagtacccgc gtgctggcag
    85201 ggatgactct gggatttttg attgtgatgt tactgtgtgc gggcatttca ttttcactgg
    85261 cagtgattga cccggcagcg gtacaccttt tgagttgggc gggggcggca tatattgtct
    85321 ggctggcgtg gaaaatcgcc accagcccaa caaaggaaga cggacttcag gcaaaaccaa
    85381 tcagcttttg ggccagcttt gctttgcagt ttgtgaacgt caaaatcatt ttgtacggtg
    85441 ttacggcact gtcgacgttt gttctgccgc aaacacaggc gctaagctgg gtagttggcg
    85501 tcagcgtttt gctggcgatg attgggacgt ttggcaatgt gtgctgggcg ctggcggggc
    85561 atctgtttca gcgattgttt cgccagtatg gtcgccagtt aaatatcgtg cttgccctgt
    85621 tgctgatcta ttgcgcggta cgcattttct attaacgaaa aaaagcggaa gaggtcgccc
    85681 tcttccgctt agtaacttgc tacttaagcc ttacaggctt tcagtaaagg tacgagcgat
    85741 aacgtcgcgc tgctgttccg gagtcagaga gttaaagcga actgcataac cggatacacg
    85801 gatggtcagc tgcggatatt tttccggatg cttaactgca tcttccagag tttcacgacg
    85861 cagaacgtta acgttcaggt gttgaccacc ttcaacgcga acttctggtt tcacttctac
    85921 tggaacttca cggtattcaa tgtcacccag tttgcttact gcaaccactt catcttctgc
    85981 ataacctgct tttgcaacga tgcaacgtgc ttcgcctttt tcgctgtcca gcagccagaa
    86041 agagttcagc agatcgtcgt tagcggcttt agtaatctgg atacctgtaa tcatgtgatg
    86101 cctccccggc aaaattattt gatttgttca gcctgtcgcg gccaattggt aaaaccattg
    86161 ttgcttgagt gtatatatac tcctcaacca cccttgattc tttgatttaa atcaataaaa
    86221 accacacatc gattgtggtc gtaaatggat tttattgttt tacatcaact tatgcgggtg
    86281 tgaaatttta ccaatttaca tttttttgca ctcgtttaag tctaaaaaat gagcatgatt
    86341 ttgttctgta gaaagaagca gttaagctag gcggattgaa gattcgcagg agagcgagat
    86401 ggctaacgaa ttaacctggc atgacgtgct ggctgaagag aagcaacaac cttattttct
    86461 taataccctt cagaccgtcg ccagcgagcg gcagtccggc gtcactatct atccaccaca
    86521 aaaagatgtc tttaacgcgt tccgctttac cgagttgggt gacgttaaag tggtgattct
    86581 cggccaggat ccttatcacg gaccgggaca ggcgcatggt ctggcatttt ccgttcgtcc
    86641 cggcattgcc attcctccgt cattattgaa tatgtataaa gagctggaaa atactattcc
    86701 gggcttcacc cgccctaatc atggctatct cgaaagctgg gcgcgtcagg gcgttctgct
    86761 gctcaatact gtgttgacgg ttcgcgcagg tcaggcgcat tcccacgcca gcctcggttg
    86821 ggagacgttt actgataaag tcatcagcct gattaaccag caccgcgaag gcgtggtgtt
    86881 tttgttgtgg ggatcacatg cgcaaaagaa aggggcgatt atagataagc aacgccatca
    86941 tgtactgaaa gcaccgcatc cgtcgccgct ttcggcgcat cgtggattct ttggctgcaa
    87001 ccattttgtg ctggcaaatc agtggctgga acaacgtggc gagacgccga ttgactggat
    87061 gccagtatta ccggcagaga gtgagtaaat ttgcggtaaa atgccggatg gcagagttgc
    87121 cacccggctg atttatcagg ctttattctg acgccaccat tcaccaagca aaacgccggt
    87181 tgcaacagag atattcagcc cggcaacgtt gcccgtaccg tcaatcttca cgcgcagatc
    87241 gttcggatcg cgtgcggcat ccggtaaccc ttcatattcc tgacccagca ccagtaccat
    87301 tttcgctggc agactggttt tgaacagcgg tttcccctgc tcgctggaag tggtcactac
    87361 ggtgtaacct gcctgacgga aatcatccaa cacgttaaca atgttgtcgc cagtaatcgg
    87421 ctgaacgtgc tctgcgccgc cttctgcggt acggatagcc gcccccgact ccagcagtgc
    87481 cgcatcctgc accacaacgc ctttcacgcc gaagtgcgcg cagctgcgca tcatgccacc
    87541 caggttatgc gggttagatt cgttttccag cgccagtaca caatcctgcg cgcctgcctg
    87601 gcttacccac tgctgcacgg ttgtgccatt acgctttttg atcaggaagc aaacgccgcc
    87661 gtgatgttcc gtgcctgacg cttttgtcaa ttccgcttca tccaccacat ggtacgcttt
    87721 gcggtttgct gccatccagc gcaaggcttc tttaaaacgc ggcgttacac tctggataaa
    87781 ccaggcgcga acaatcgctt ccggacggct ctggaaaagt gcctgacatg cattttcgcc
    87841 gtagacgcgg gtttcttccg cacgctgacg acgcaacact tccggatcaa taaaactttt
    87901 accactgatg ccaccgtgat cggccttttc cggcgtctca tcacccggcg cgcgggaaac
    87961 cgtgcgccac ggcgaatctt cccacttgcg gtcacggggc tgactctgtt tgtcatcgcg
    88021 ggcggggcga cggccaccgt cagcacgaga ttttcctgga cgcccgcccc ctttcccggt
    88081 acgcgggttg tgggtacgtt tatcagaatc atcatcactg cggacataca tcactttgac
    88141 cttgccgctt ttacctttca tttcatcgtt catgcttttc tccaccagcg ctgcgcgaag
    88201 cgcgcagatt acccgaagtc cgcgcggttc gccatgattt cgtaccaaag cctgcgacta
    88261 tcatacctat tgaataaaac agattgttgt ctggaacaat gtccccgata atatgtaaca
    88321 tattagaaac ataccggcgt cgttgccgat aagtctcctt actcatcccg aggttagtta
    88381 tgaataccgt ttgtacccat tgtcaggcca tcaatcgcat tcccgacgat cggatcgaag
    88441 atgcggcaaa atgcggacgc tgcggtcacg acttgtttga cggagaggtg attaatgcga
    88501 ccggtgaaac actcgacaaa ttgctgaagg atgatctacc tgtggtgatc gacttctggg
    88561 caccgtggtg cggcccctgc cgtaatttcg caccaatttt tgaagatgtc gcgcaagagc
    88621 gtagcggtaa agtgcgcttt gtgaaagtga ataccgaagc tgaacgtgaa ttgagcagtc
    88681 gctttggaat tcgtagtata ccgacgatca tgattttcaa aaaccgtcag gttgtcgaca
    88741 tgcttaatgg cgcagtaccg aaagcgccgt tcgatagctg gctgaacgaa tctctttaat
    88801 cttaccgggg cgcatcttgt gccccgtttt ctcctctgcg acaatggcgt tttttcgacg
    88861 ctctcttatg accgaaaacg ctgttctcca gttacgcgcc gagcgtattg cgcgcgcaac
    88921 acgtcctttt cttgcccgcg gtaatcgcgt tcgtcgctgc caacgctgtc ttttgccaga
    88981 gaaattatgt ctctgttcga caattacccc agcacaagca aaaagtcgct tctgtttgct
    89041 gatgttcgac accgagccaa tgaagcccag taataccggg cgtctgattg ctgatatttt
    89101 gcctgatacc gttgcgtttc aatggtcgcg taccgaaccc tcgcaggatt tgctggatct
    89161 ggtacaaaac ccggattatc agccaatggt ggtctttccc gcctcgtatg ctgatgagca
    89221 acgggaagtg atcttcacac cacctgccgg taagccaccg ctgtttatca tgctcgatgg
    89281 tacctggccg gaagctcgca agatgtttcg taaaagtccg tatctggata atcttcccgt
    89341 catttccgtc gatctttccc ggctttctgc ctatcgcctg cgtgaagccc aggctgcagg
    89401 ccaatattgt actgccgagg tagccatcgc actgttagat atggccggcg ataccggggc
    89461 ggcggcaggg ttaggcgagc attttacccg ctttaaaaca cgctatctgg caggaaaaac
    89521 gcaacatctg ggtagcatca cagcagaaca attagaaagc gtttaaaatc attcggtcac
    89581 ttctgcggga gaccggtatg agtcagcgag gactggaagc actactgcga ccaaaatcga
    89641 tagcggtaat tggcgcgtcg atgaaaccca atcgcgcagg ttacctgatg atgcgtaacc
    89701 tgctggcggg aggctttaac ggaccggtac tcccggtgac gccagcctgg aaagcggtgt
    89761 tgggtgtgtt ggcctggccg gatattgcca gcttgccctt tacacccgac cttgcggttt
    89821 tatgtaccaa tgccagccgt aatcttgctc ttctggaaga gctcggcgag aaaggctgta
    89881 aaacctgcat tattctttcc gccccggcat cgcaacacga agatctccgc gcctgcgccc
    89941 tgcgccataa catgcgcctg cttggaccaa acagtctggg tttactggct ccctggcaag
    90001 gtctgaatgc cagcttttcg cctgtgccga ttaaacgcgg caagctggcg tttatttcac
    90061 aatcggctgc cgtctccaac accatcctcg actgggcgca acagcgtgag atgggctttt
    90121 cctactttat tgcgctcggc gacagcctgg atatcgacgt tgatgaattg ctcgactatc
    90181 ttgcgcgcga cagtaaaacc agcgccatcc tgctctatct cgaacagtta agcgacgcgc
    90241 gacgctttgt ttcggcggcc cgtagtgcct cgcgtaataa accgattctg gtgattaaaa
    90301 gcggacgtag cccggcggct caacgtctgt tgaacaccac ggcaggaatg gatccggcgt
    90361 gggatgcggc tattcagcgt gccggtttgt tgcgggtaca ggacacccac gagctgtttt
    90421 cggcggtgga aacccttagc catatgcgcc cgctgcgtgg cgaccggttg atgattatca
    90481 gcaacggtgc tgcgcctgcc gcgctggcgc tggatgcctt atggtcacac aatggcaagc
    90541 tggcaacgct aagcgaagaa acctgccaga aactgcgcga tgcactgcct ggacatgtgg
    90601 caatctctaa cccgctcgat ctgcgcgatg acgccagcag cgagcattat gtcaaaacgc
    90661 tggacattct gctccacagc caggattttg atgcgctgat ggttattcat tcacccagcg
    90721 cagcggctcc cgcaacagaa agcgcgcaag aattaattga agcggtaaag catcatcccc
    90781 gcagcaaata tgtctctttg ctgacgaact ggtgcggcga gcactcctcg caagaggcac
    90841 gacgtttatt cagcgaagcc gggctgccga cctaccgtac cccggaagga accatcactg
    90901 cttttatgca tatggtggag taccggcgta atcagaagca actacgcgaa acgccggcgt
    90961 tgcccagcaa tctgacctcc aataccgcag aagcgcatct tctgttgcaa caggcgattg
    91021 ccgaaggagc aacgtcactc gatacccatg aagttcagcc tatcttgcaa gcatatggca
    91081 tgaacacgct ccccacctgg attgccagcg atagcaccga agcggtgcat attgccgaac
    91141 agattggtta tccggtggcg ctgaaattgc gttcgccgga tattccacat aaatcggaag
    91201 ttcagggcgt catgctctac ctgcgtacag ccaatgaagt ccagcaagcg gcaaacgcca
    91261 ttttcgatcg cgtaaaaatg gcctggccgc aggcacgggt ccacggcctg ttggtgcaaa
    91321 gtatggctaa ccgtgctggt gctcaggagt tgcgggttgt ggttgagcac gatccggttt
    91381 tcgggccgtt gatcatgctg ggcgaaggcg gtgtggagtg gcgtcctgaa gatcaagccg
    91441 tcgtcgcgtt accgccgctg aacatgaacc tggcccgcta tctggttatt caggggatca
    91501 aaagtaaaaa gattcgtgca cgcagtgcgc tacgcccatt ggatgttgca ggcttgagcc
    91561 agcttctggt gcaggtttcc aacttgattg tcgattgccc ggaaattcag cgtctggata
    91621 ttcatccttt gctggcttct ggcagtgaat ttaccgcgct ggatgtcacg ctggatatct
    91681 cgccgtttga aggcgataac gagagtcggc tggcagtgcg cccttatccg catcagctgg
    91741 aagaatgggt agaattgaaa aacggtgaac gctgcttgtt ccgcccgatt ttgccagaag
    91801 atgagccaca acttcagcaa ttcatttcgc gagtcaccaa agaagatctt tattaccgct
    91861 actttagcga gatcaacgaa tttacccatg aagatttagc caacatgaca cagatcgact
    91921 acgatcggga aatggcgttt gtagcggtac gacgtattga tcaaacggaa gagatcctcg
    91981 gcgtcacgcg tgcgatttcc gatcctgata acatcgatgc cgaatttgct gtgctggttc
    92041 gctcggatct caaagggtta ggcttaggtc gacgcttaat ggaaaagttg attacctata
    92101 cgcgagatca cggactacaa cgtctgaatg gtattacgat gccaaacaat cgtggcatgg
    92161 tggcgctagc ccgcaagctc gggtttaacg ttgatatcca gctcgaagag gggatcgttg
    92221 ggcttacgct aaatcttgcc caacgcgagg aatcatgagt aaggtactgg aaatgttgac
    92281 cacttaatcg ggactggtgt tattattgcc cgcttatgtc gtctgcattg cacagaggac
    92341 ccttcaatga acagagaaga aatgcactgt gatgttgtca aaatttaagc gtaataaaca
    92401 tcaacaacac cttgcccaac tacccaagat ttctcaatca gttgatgatg tcgatttctt
    92461 ttacgctccc gccgacttcc gggagacgct gctggaaaaa atagccagcg cgaagcagcg
    92521 catttgcatt gtcgccctgt atctcgaaca ggatgacggt ggcaaaggca ttctgaacgc
    92581 gttgtatgaa gctaaaaggc agcgtccgga actggatgtg cgggtgctgg tcgactggca
    92641 tcgtgcacaa cgtggacgca ttggcgctgc ggcatctaac actaacgctg actggtactg
    92701 ccgcatgacg caggaaaatc cgggcgtaga tgttccggtt tatggcgttc caatcaatac
    92761 ccgtgaagcc cttggtgttc tgcactttaa aggctttatc atcgacgata gcgtacttta
    92821 tagcggtgcc agcctgaacg atgtttacct gcatcagcac gataaatatc gctacgaccg
    92881 ttatcatctt atccgtaacc gtaagatgtc agacattatg tttgaatggg ttacacagaa
    92941 tattatgaat ggccgcggcg ttaatcgtct ggatgatgtt aatcggccaa aaagcccgga
    93001 aatcaagaac gatattcgtc tgttccgcca ggagctgcgt gatgccgctt atcatttcca
    93061 gggcgatgcc gacaacgatc agctttccgt aacgccgcta gtggggctgg ggaaatcgag
    93121 tctgttgaac aagaccattt tccatcttat gccttgtgcc gagcagaaac taaccatctg
    93181 tacgccatac ttcaacctgc cagcaatcct tgtgcgcaat attatccagt tgctgcgcga
    93241 agggaaaaag gtcgaaatta ttgttggtga taaaaccgcg aacgacttct acattccgga
    93301 agatgaacct ttcaagataa ttggcgcatt gccttatctc tatgagatca atctgcgtcg
    93361 tttcctgagc cgtttgcagt attacgtcaa tactgaccag ctagtggttc ggttatggaa
    93421 agatgacgac aacacctatc acctaaaagg gatgtgggtt gatgataagt ggatgttgat
    93481 caccggtaat aacctgaacc cgcgcgcctg gcgtctggat ctggaaaacg ccattttgat
    93541 ccacgatcca caactggagc ttgcgccgca gcgagagaaa gaactggagc tgatccgcga
    93601 gcataccacc atcgttaagc actatcgcga tctgcaaagt attgccgatt atccggtgaa
    93661 ggttcgtaaa ctcatccgcc gtttgcgccg tatccgcatc gaccgattaa ttagccgcat
    93721 cctgtaatca caaccccgtc ctgtacgggg tttgtttttt ggaggccacg ttttgcgtat
    93781 tctttttgtc tgttcgctat tgttactttc tggatgcagc catatggcta acgatagctg
    93841 gagcgggcag gataaagctc aacactttat cgcctcggcg atgctttccg ccgccggaaa
    93901 tgaatattca cagcatcagg ggatgagccg ggatcgcagt gccatgtttg gattgatgtt
    93961 ctctgtcagt ttgggggcgt caaaagagct ttgggatagc cgccccgaag ggagcggctg
    94021 gagctggaag gatttggcct gggatgtcgc cggtgcaagc accggctata ccgtctggca
    94081 actgacccgt cactaaagac gcatcccctt ccctttgcga tgtagcatca aagaaaccag
    94141 aaacgccacc acggccatca aggtcacata ccagaagaag gctgtttcca ttcctattga
    94201 tttcagcgac aacgctacgt actccgccga accaccaaat atagcattag cgaccgcata
    94261 tgacaggcca acgcctaatg cgcgaacctg tgccgggaac atctcagcct tcagtattcc
    94321 actgattgat gtataaaaac tcactatcag cagggcacac atcaccagac caaaagcggc
    94381 ataaggcgag gaaacgtttt gcaatgctga gagaatagga acggtaaaaa tggctgccag
    94441 cgaaccgaaa cataacattg aggtacggcg accaatctta tccgacagcg cgccaatgag
    94501 tggttgaata agcatgaata caaacaatgc ggcagtcata atgccactcg ccacgttggc
    94561 atgcattccc gcagtattta ccagatactt ctgcatataa gtggtgaagg tatagaaaca
    94621 aagggagccc gcagcggtaa aaccgagaac catgatgaat gcacggcgat tgcgccataa
    94681 tcctttcaga gatccagctt cttttaaagc gcgcgtttct tgttgcgaag tttcatctaa
    94741 ttgacgacgt aaccacaacg ccacaacagc taacacagct cctaacgcga aaggaatacg
    94801 ccatccccac tctctgagtg cagcgtcttc catggtgtgt tgtaaaacca cgacaaccag
    94861 tagggctagc agttgtccgc cgatcaacgt cacatactga aatgatgcgt aaaaaccttt
    94921 gcgcccttca acggcaactt cactcatata ggtggcgctg gtgccatatt ctccgccaac
    94981 ggataatccc tgaaataaac gagcgagaag caataatgcc ggagcccacg tacctatagt
    95041 ttcataacct gggaggcagg cgataaccag cgatccgaaa cacatcatac acaccgataa
    95101 cagcatcgat tttttgcgac catgtttatc ggctatgcgg ccaaatagcc aaccgcctat
    95161 tgggcgcatc aggaatcccg cagcaaaaac acctgctgtt tgtagtagtt gagtcgtcgt
    95221 gttcccggaa gggaagaaaa tgtgggcgaa gtagagtgaa cagaacgagt agacatagaa
    95281 atcgaaccac tcgaccagat tacctgaaga ggcccccaca atcgcccaaa tgcggcgacg
    95341 tgtatcacta cttgtcagtt tgctgtctgc cgttacagta ctttcagcca tgccattatg
    95401 tctcctgccg taatccgatg cttttgtcgg tcgcttttgt ttatttcttt tggtaaagaa
    95461 aatgttatac atttgttgcg tatcattatg caaccttaac catgaattta gttagcagga
    95521 aaatggttat tgaggagctt aaggataaat ttctggtgag gagaacacgt atggaggtgg
    95581 gcaagttggg gaagccgtat tcgctgctga atctggcata tgtgggagta taagacgcgc
    95641 agcgtcgcat c
//
LOCUS       DAZGVQ010000002        93232 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000002 DAZGVQ010000000
VERSION     DAZGVQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 93232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 93232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..93232
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.002"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..331)
                     /locus_tag="ACHLAG_000087"
     CDS             complement(<1..331)
                     /locus_tag="ACHLAG_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP0653401.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQND"
     gene            401..763
                     /gene="tnpA"
                     /locus_tag="ACHLAG_000088"
                     /pseudo
     CDS             401..763
                     /gene="tnpA"
                     /locus_tag="ACHLAG_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(862..1530)
                     /gene="ydcL"
                     /locus_tag="ACHLAG_000089"
     CDS             complement(862..1530)
                     /gene="ydcL"
                     /locus_tag="ACHLAG_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFP0653402.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(1833..2426)
                     /gene="tehB"
                     /locus_tag="ACHLAG_000090"
     CDS             complement(1833..2426)
                     /gene="tehB"
                     /locus_tag="ACHLAG_000090"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFP0653403.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(2423..3415)
                     /gene="tehA"
                     /locus_tag="ACHLAG_000091"
     CDS             complement(2423..3415)
                     /gene="tehA"
                     /locus_tag="ACHLAG_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFP0653404.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            3539..4519
                     /gene="ydcK"
                     /locus_tag="ACHLAG_000092"
     CDS             3539..4519
                     /gene="ydcK"
                     /locus_tag="ACHLAG_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFP0653405.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHALVEAFDGDTVHVRGPKVINGEGRITRTPLAGLL"
     gene            complement(4514..5050)
                     /gene="rimL"
                     /locus_tag="ACHLAG_000093"
     CDS             complement(4514..5050)
                     /gene="rimL"
                     /locus_tag="ACHLAG_000093"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFP0653406.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTVEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(5113..5337)
                     /gene="ydcH"
                     /locus_tag="ACHLAG_000094"
     CDS             complement(5113..5337)
                     /gene="ydcH"
                     /locus_tag="ACHLAG_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFP0653407.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(5477..7132)
                     /gene="opgD"
                     /locus_tag="ACHLAG_000095"
                     /gene_synonym="mdoD"
     CDS             complement(5477..7132)
                     /gene="opgD"
                     /locus_tag="ACHLAG_000095"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFP0653408.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(7357..8700)
                     /gene="ydcJ"
                     /locus_tag="ACHLAG_000096"
                     /pseudo
     CDS             complement(7357..8700)
                     /gene="ydcJ"
                     /locus_tag="ACHLAG_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            8917..9840
                     /gene="ydcI"
                     /locus_tag="ACHLAG_000097"
     CDS             8917..9840
                     /gene="ydcI"
                     /locus_tag="ACHLAG_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0653409.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEDLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(9878..11518)
                     /gene="trg"
                     /locus_tag="ACHLAG_000098"
     CDS             complement(9878..11518)
                     /gene="trg"
                     /locus_tag="ACHLAG_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFP0653410.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            11917..12066
                     /gene="hokB"
                     /locus_tag="ACHLAG_000099"
     CDS             11917..12066
                     /gene="hokB"
                     /locus_tag="ACHLAG_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFP0653411.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(12044..12112)
                     /gene="yncP"
                     /locus_tag="ACHLAG_000100"
     CDS             complement(12044..12112)
                     /gene="yncP"
                     /locus_tag="ACHLAG_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFP0653412.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(12138..12311)
                     /locus_tag="ACHLAG_000101"
     CDS             complement(12138..12311)
                     /locus_tag="ACHLAG_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0653413.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(12556..13086)
                     /gene="cybB"
                     /locus_tag="ACHLAG_000102"
     CDS             complement(12556..13086)
                     /gene="cybB"
                     /locus_tag="ACHLAG_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFP0653414.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            13275..14276
                     /gene="gap"
                     /locus_tag="ACHLAG_000103"
     CDS             13275..14276
                     /gene="gap"
                     /locus_tag="ACHLAG_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP0653415.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSISVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(14318..15757)
                     /gene="aldA"
                     /locus_tag="ACHLAG_000104"
     CDS             complement(14318..15757)
                     /gene="aldA"
                     /locus_tag="ACHLAG_000104"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFP0653416.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(15954..16754)
                     /gene="ydcF"
                     /locus_tag="ACHLAG_000105"
     CDS             complement(15954..16754)
                     /gene="ydcF"
                     /locus_tag="ACHLAG_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFP0653417.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQEPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(17026..20928)
                     /gene="hrpA"
                     /locus_tag="ACHLAG_000106"
     CDS             complement(17026..20928)
                     /gene="hrpA"
                     /locus_tag="ACHLAG_000106"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFP0653418.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            21129..21734
                     /gene="azoR"
                     /locus_tag="ACHLAG_000107"
     CDS             21129..21734
                     /gene="azoR"
                     /locus_tag="ACHLAG_000107"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFP0653419.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(21785..23101)
                     /gene="ynbD"
                     /locus_tag="ACHLAG_000108"
     CDS             complement(21785..23101)
                     /gene="ynbD"
                     /locus_tag="ACHLAG_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFP0653420.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIEWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(23091..24848)
                     /gene="ynbC"
                     /locus_tag="ACHLAG_000109"
     CDS             complement(23091..24848)
                     /gene="ynbC"
                     /locus_tag="ACHLAG_000109"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFP0653421.1"
                     /translation="MENSRIHGEHFFTSSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTVFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(24864..25760)
                     /gene="ynbB"
                     /locus_tag="ACHLAG_000110"
     CDS             complement(24864..25760)
                     /gene="ynbB"
                     /locus_tag="ACHLAG_000110"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP0653422.1"
                     /translation="MLEKSLATLFALLILATLINHFLLWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(25760..26365)
                     /gene="ynbA"
                     /locus_tag="ACHLAG_000111"
     CDS             complement(25760..26365)
                     /gene="ynbA"
                     /locus_tag="ACHLAG_000111"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP0653423.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            complement(26536..28842)
                     /gene="ydbD"
                     /locus_tag="ACHLAG_000112"
     CDS             complement(26536..28842)
                     /gene="ydbD"
                     /locus_tag="ACHLAG_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFP0653424.1"
                     /translation="MFKARICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(28906..29766)
                     /gene="pdxI"
                     /locus_tag="ACHLAG_000113"
     CDS             complement(28906..29766)
                     /gene="pdxI"
                     /locus_tag="ACHLAG_000113"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFP0653425.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVIMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALVWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(29997..30587)
                     /gene="paaY"
                     /locus_tag="ACHLAG_000114"
     CDS             complement(29997..30587)
                     /gene="paaY"
                     /locus_tag="ACHLAG_000114"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFP0653426.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            complement(30569..31519)
                     /gene="paaX"
                     /locus_tag="ACHLAG_000115"
     CDS             complement(30569..31519)
                     /gene="paaX"
                     /locus_tag="ACHLAG_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFP0653427.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(31620..32933)
                     /gene="paaK"
                     /locus_tag="ACHLAG_000116"
     CDS             complement(31620..32933)
                     /gene="paaK"
                     /locus_tag="ACHLAG_000116"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFP0653428.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRCKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(32960..34165)
                     /gene="paaJ"
                     /locus_tag="ACHLAG_000117"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(32960..34165)
                     /gene="paaJ"
                     /locus_tag="ACHLAG_000117"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFP0653429.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(34165..34587)
                     /gene="paaI"
                     /locus_tag="ACHLAG_000118"
     CDS             complement(34165..34587)
                     /gene="paaI"
                     /locus_tag="ACHLAG_000118"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFP0653430.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(34577..36004)
                     /gene="paaH"
                     /locus_tag="ACHLAG_000119"
     CDS             complement(34577..36004)
                     /gene="paaH"
                     /locus_tag="ACHLAG_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFP0653431.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAANLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(36006..36794)
                     /gene="paaG"
                     /locus_tag="ACHLAG_000120"
     CDS             complement(36006..36794)
                     /gene="paaG"
                     /locus_tag="ACHLAG_000120"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFP0653432.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(36794..37561)
                     /gene="paaF"
                     /locus_tag="ACHLAG_000121"
     CDS             complement(36794..37561)
                     /gene="paaF"
                     /locus_tag="ACHLAG_000121"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFP0653433.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSAGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(37558..38628)
                     /gene="paaE"
                     /locus_tag="ACHLAG_000122"
     CDS             complement(37558..38628)
                     /gene="paaE"
                     /locus_tag="ACHLAG_000122"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFP0653434.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(38636..39133)
                     /gene="paaD"
                     /locus_tag="ACHLAG_000123"
     CDS             complement(38636..39133)
                     /gene="paaD"
                     /locus_tag="ACHLAG_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFP0653435.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(39148..39894)
                     /gene="paaC"
                     /locus_tag="ACHLAG_000124"
     CDS             complement(39148..39894)
                     /gene="paaC"
                     /locus_tag="ACHLAG_000124"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFP0653436.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQCVLPGQQW"
     gene            complement(39903..40190)
                     /gene="paaB"
                     /locus_tag="ACHLAG_000125"
     CDS             complement(39903..40190)
                     /gene="paaB"
                     /locus_tag="ACHLAG_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFP0653437.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(40202..41131)
                     /gene="paaA"
                     /locus_tag="ACHLAG_000126"
     CDS             complement(40202..41131)
                     /gene="paaA"
                     /locus_tag="ACHLAG_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFP0653438.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTIPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            41416..43461
                     /gene="paaZ"
                     /locus_tag="ACHLAG_000127"
     CDS             41416..43461
                     /gene="paaZ"
                     /locus_tag="ACHLAG_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFP0653439.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQRHALQLACAGGGSLAGTLVTADPQIARQFITDAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLLHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            43709..45982
                     /gene="tynA"
                     /locus_tag="ACHLAG_000128"
     CDS             43709..45982
                     /gene="tynA"
                     /locus_tag="ACHLAG_000128"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFP0653440.1"
                     /translation="MGSPSLYSARKTALALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQSLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGAVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(46040..47539)
                     /gene="feaB"
                     /locus_tag="ACHLAG_000129"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(46040..47539)
                     /gene="feaB"
                     /locus_tag="ACHLAG_000129"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HFP0653441.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            47775..48680
                     /gene="feaR"
                     /locus_tag="ACHLAG_000130"
     CDS             47775..48680
                     /gene="feaR"
                     /locus_tag="ACHLAG_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFP0653442.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTSGVNLSRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(48852..49181)
                     /gene="ydbL"
                     /locus_tag="ACHLAG_000131"
     CDS             complement(48852..49181)
                     /gene="ydbL"
                     /locus_tag="ACHLAG_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFP0653443.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(49186..49371)
                     /gene="ynbE"
                     /locus_tag="ACHLAG_000132"
     CDS             complement(49186..49371)
                     /gene="ynbE"
                     /locus_tag="ACHLAG_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFP0653444.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(49368..52007)
                     /gene="ydbH"
                     /locus_tag="ACHLAG_000133"
     CDS             complement(49368..52007)
                     /gene="ydbH"
                     /locus_tag="ACHLAG_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFP0653445.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYYHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            52215..53204
                     /gene="ldhA"
                     /locus_tag="ACHLAG_000134"
     CDS             52215..53204
                     /gene="ldhA"
                     /locus_tag="ACHLAG_000134"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP0653446.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            53315..53737
                     /gene="hslJ"
                     /locus_tag="ACHLAG_000135"
     CDS             53315..53737
                     /gene="hslJ"
                     /locus_tag="ACHLAG_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFP0653447.1"
                     /translation="MKKVAALVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTNDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(53734..54000)
                     /gene="ydbJ"
                     /locus_tag="ACHLAG_000136"
     CDS             complement(53734..54000)
                     /gene="ydbJ"
                     /locus_tag="ACHLAG_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFP0653448.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            54274..57798
                     /gene="nifJ"
                     /locus_tag="ACHLAG_000137"
     CDS             54274..57798
                     /gene="nifJ"
                     /locus_tag="ACHLAG_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFP0653449.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLVQMAGKAEKSNTD"
     gene            58165..59298
                     /gene="ompN"
                     /locus_tag="ACHLAG_000138"
     CDS             58165..59298
                     /gene="ompN"
                     /locus_tag="ACHLAG_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFP0653450.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLINGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            59439..59873
                     /gene="uspF"
                     /locus_tag="ACHLAG_000139"
     CDS             59439..59873
                     /gene="uspF"
                     /locus_tag="ACHLAG_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFP0653451.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            60049..60984
                     /gene="ttcA"
                     /locus_tag="ACHLAG_000140"
     CDS             60049..60984
                     /gene="ttcA"
                     /locus_tag="ACHLAG_000140"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFP0653452.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(61113..62485)
                     /gene="dbpA"
                     /locus_tag="ACHLAG_000141"
                     /pseudo
     CDS             complement(61113..62485)
                     /gene="dbpA"
                     /locus_tag="ACHLAG_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
     gene            complement(62515..62688)
                     /gene="ynaL"
                     /locus_tag="ACHLAG_000142"
     CDS             complement(62515..62688)
                     /gene="ynaL"
                     /locus_tag="ACHLAG_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFP0653453.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(62963..63946)
                     /gene="zntB"
                     /locus_tag="ACHLAG_000143"
     CDS             complement(62963..63946)
                     /gene="zntB"
                     /locus_tag="ACHLAG_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFP0653454.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(63960..64007)
                     /gene="ydaN"
                     /locus_tag="ACHLAG_000144"
     CDS             complement(63960..64007)
                     /gene="ydaN"
                     /locus_tag="ACHLAG_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFP0653455.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            64201..65433
                     /gene="dgcM"
                     /locus_tag="ACHLAG_000145"
     CDS             64201..65433
                     /gene="dgcM"
                     /locus_tag="ACHLAG_000145"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFP0653456.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(65454..66017)
                     /gene="smrA"
                     /locus_tag="ACHLAG_000146"
     CDS             complement(65454..66017)
                     /gene="smrA"
                     /locus_tag="ACHLAG_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFP0653457.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(66347..67255)
                     /gene="abgR"
                     /locus_tag="ACHLAG_000147"
     CDS             complement(66347..67255)
                     /gene="abgR"
                     /locus_tag="ACHLAG_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0653458.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            67431..68741
                     /gene="abgA"
                     /locus_tag="ACHLAG_000148"
     CDS             67431..68741
                     /gene="abgA"
                     /locus_tag="ACHLAG_000148"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFP0653459.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            68741..70186
                     /gene="abgB"
                     /locus_tag="ACHLAG_000149"
     CDS             68741..70186
                     /gene="abgB"
                     /locus_tag="ACHLAG_000149"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFP0653460.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFASAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            70220..71746
                     /gene="abgT"
                     /locus_tag="ACHLAG_000150"
     CDS             70220..71746
                     /gene="abgT"
                     /locus_tag="ACHLAG_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFP0653461.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDVLESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            71757..72272
                     /gene="ogt"
                     /locus_tag="ACHLAG_000151"
     CDS             71757..72272
                     /gene="ogt"
                     /locus_tag="ACHLAG_000151"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFP0653462.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            72467..73219
                     /gene="fnr"
                     /locus_tag="ACHLAG_000152"
     CDS             72467..73219
                     /gene="fnr"
                     /locus_tag="ACHLAG_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFP0653463.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            73371..74321
                     /gene="uspE"
                     /locus_tag="ACHLAG_000153"
     CDS             73371..74321
                     /gene="uspE"
                     /locus_tag="ACHLAG_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFP0653464.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(74371..74628)
                     /gene="ynaJ"
                     /locus_tag="ACHLAG_000154"
     CDS             complement(74371..74628)
                     /gene="ynaJ"
                     /locus_tag="ACHLAG_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFP0653465.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            74872..75903
                     /gene="ynaI"
                     /locus_tag="ACHLAG_000155"
     CDS             74872..75903
                     /gene="ynaI"
                     /locus_tag="ACHLAG_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFP0653466.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPQEQGR"
     gene            complement(75954..77567)
                     /gene="mppA"
                     /locus_tag="ACHLAG_000156"
     CDS             complement(75954..77567)
                     /gene="mppA"
                     /locus_tag="ACHLAG_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFP0653467.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(77904..78803)
                     /gene="pgrR"
                     /locus_tag="ACHLAG_000157"
     CDS             complement(77904..78803)
                     /gene="pgrR"
                     /locus_tag="ACHLAG_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFP0653468.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSAMASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            78941..79861
                     /locus_tag="ACHLAG_000158"
     CDS             78941..79861
                     /locus_tag="ACHLAG_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0653469.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVLSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            79861..80793
                     /locus_tag="ACHLAG_000159"
     CDS             79861..80793
                     /locus_tag="ACHLAG_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0653470.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            80811..81449
                     /locus_tag="ACHLAG_000160"
     CDS             80811..81449
                     /locus_tag="ACHLAG_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0653471.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGDNIGINQPVTDGDKPLFM"
     gene            81758..82486
                     /gene="mpaA"
                     /locus_tag="ACHLAG_000161"
     CDS             81758..82486
                     /gene="mpaA"
                     /locus_tag="ACHLAG_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFP0653472.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     ATNWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(82461..83426)
                     /gene="ycjG"
                     /locus_tag="ACHLAG_000162"
     CDS             complement(82461..83426)
                     /gene="ycjG"
                     /locus_tag="ACHLAG_000162"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFP0653473.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGSTWLAVDVEPALQFTTGELHL"
     gene            83545..84051
                     /gene="tpx"
                     /locus_tag="ACHLAG_000163"
     CDS             83545..84051
                     /gene="tpx"
                     /locus_tag="ACHLAG_000163"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFP0653474.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(84095..85636)
                     /gene="tyrR"
                     /locus_tag="ACHLAG_000164"
     CDS             complement(84095..85636)
                     /gene="tyrR"
                     /locus_tag="ACHLAG_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFP0653475.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(85784..86845)
                     /gene="ycjF"
                     /locus_tag="ACHLAG_000165"
     CDS             complement(85784..86845)
                     /gene="ycjF"
                     /locus_tag="ACHLAG_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFP0653476.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(86842..88239)
                     /gene="ycjX"
                     /locus_tag="ACHLAG_000166"
     CDS             complement(86842..88239)
                     /gene="ycjX"
                     /locus_tag="ACHLAG_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFP0653477.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTNDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            88395..89393
                     /gene="ycjW"
                     /locus_tag="ACHLAG_000167"
     CDS             88395..89393
                     /gene="ycjW"
                     /locus_tag="ACHLAG_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0653478.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNLAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(89504..90409)
                     /gene="ompG"
                     /locus_tag="ACHLAG_000168"
     CDS             complement(89504..90409)
                     /gene="ompG"
                     /locus_tag="ACHLAG_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFP0653479.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(90454..91536)
                     /locus_tag="ACHLAG_000169"
     CDS             complement(90454..91536)
                     /locus_tag="ACHLAG_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0653480.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(91550..92209)
                     /gene="pgmB"
                     /locus_tag="ACHLAG_000170"
     CDS             complement(91550..92209)
                     /gene="pgmB"
                     /locus_tag="ACHLAG_000170"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP0653481.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(92206..>93232)
                     /gene="ycjT"
                     /locus_tag="ACHLAG_000171"
     CDS             complement(92206..>93232)
                     /gene="ycjT"
                     /locus_tag="ACHLAG_000171"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFP0653482.1"
                     /translation="HLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAKFWISRAVR
                     VNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDAFIHRAEMF
                     LKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEVNEMQILKQ
                     ADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGLLTQSYQFW
                     REGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPALPEQWQQL
                     SFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDFILPFNGTA
                     TTHQEDE"
BASE COUNT        23644 a        24187 c        22230 g        23171 t
ORIGIN      
        1 catcattctg tccccgcttt ttgaatcggg gaaaagcagc ccgcttccgg aagaagtttt
       61 tgtaagcccg ctcaaggtct ttcagtgact gttgcaatgg ctgtgagggc gaatctttaa
      121 gccattgcgt ttcagtggcg tttttccact caaccagcca ggaagccatt ttcccgtaag
      181 ggatgtattt attaccggcc tcatgattct cattctgaag cgccagagca cgattgaaaa
      241 cgaaacgaca tgcgcctgcg aagcgcctca tttcacgctc ctgttgacca ccgggtctta
      301 actggaattt aaatgcctgt aatcgtttca caggccaaat gatactttgg tctatgaata
      361 aagaaaccga tatccgacgg ggaagcattg tgttttcctg atgcatgttc acctggtctt
      421 tgtcaccaga taccgacgtc agatttttga ctatgacgcg acagaaaaac tacgcactta
      481 cttttcaaat gtatgtgctg attttgaagc tgaactggtt taaatggatg gcgaaccaga
      541 tcacgtccat ttgttaatta actatcctcc caaactggcg atatccagtc tggtaaacag
      601 cctcaaaggc gtatcgagta ggttactgcg acgagatcga ccagatattg cagtcaggta
      661 ttactacaaa ggcgttttgt ggagtcctgg ctattttgcc agtagctgcg gaggtgcgcc
      721 aatatccgtc atccgccaat acattgaaca acagcaaaca cctggtcagg tggaaaaccg
      781 cgccttatat ccccgccctg aaggatgggg ttttacggcg caccggataa aaagcgccat
      841 ctaacgatgg cgcgtctgga actacttttt gttaacgtca aacatggtgg catcggtcgc
      901 catgtcatca ataacttgtt taatattttc gaaagccatt ggcgtacttt cgttattcag
      961 gtctttacct tcgccctgac gaacgacttt gataaccggt ttattagtcg ctgcatcaat
     1021 cagctcacct tcaaaataga ggcgagtatc catggtacgg tggcctgtag ccatttgcgt
     1081 ccccgcaacc actaatgcaa caggaaccac ttcatagaat tgcagccctt ctttgctggt
     1141 atctacaccg gtaatggccc cacggaaaat cagactacgc ggcccagcgg tggtaaccag
     1201 tggtttacgc tgcgctatcg cttctttcat ttcggtgttg gtatagttca aaattttatc
     1261 cagaactttc tgccctacct gggtagaagg ttttggtacc ggataataag tgattgggtt
     1321 ccagacgatg ctgtcatatt tgctttgatc aaaactcggg tctacccaac gtaaaacagg
     1381 tttacctgtt gctgaggttg tttcttttaa atcagagtaa ttgtttaaaa aaccagaata
     1441 tttatctggc tgggtgattt tagaggcaca accagacaga gccaacaagc cgcataaagc
     1501 tgcaactttc gcaaatgatg tggtacgcat gaatagtttc ctgagatgat tatattactt
     1561 tctaagttat agcaaaaagc gccgcttcgt gttgtggcaa aaaaagtgtt ggtgaaaata
     1621 aatttttcag ccccttttca gacgacatag tcaatgccga aaaaggggct aagtcaatca
     1681 tcagtagtta aatttcagta agttaagctg ctaaaaaact tcgactactg tgattaattt
     1741 cacatccctt cgataaaaaa tatgcttacc gaaaagaaga acagtaaatc atctcagcgt
     1801 ggggaatttt cacgcgcctt attcttagcg ggtcattttt tacgtgccag catcgtggcg
     1861 aaacgcagtt taatacgatt accgttggcg tcggtgcggt gcagctcgcc gacgtcttca
     1921 ttgtatttca ccctctccca gccttcgtaa tatcgacgta attctccctc tttgaaggca
     1981 aacgggaagc cgacggtaca tggataatca gcggtatcca tcgccgccac aatcaggttg
     2041 taaccaccag gtttagtgca acgttgcata ttggcaatca gccctgggat ggttttagcc
     2101 tcaaggaaca tcagtaccac agtcgaaaga ataaaatcgt actgtctatc aaatgtgagg
     2161 ttattcagat caacgactcg ggtgtgtaaa ttatccagat tttcaatgga tttaatgcgc
     2221 tcgacgttgg cgatactcat ggcattttta tcccatgcgt caacatcata accattggct
     2281 gccaggtaaa gactgttacg accattgcca cagcccagat ccagcgtttt acccggttta
     2341 accactttca ccgcttccag tacttcagag tgtgtgcggg ttaattcata tttatcagta
     2401 aaatagtttt cgtcacgaat gatcattctt tgtcctctgc tttcattaaa acggcgcgct
     2461 cggttctgac taacaatttt ccctgcatca gaagcgcaaa agtacggatg agcagtattg
     2521 caataataaa attggtaaag ataaacagcg gtaccgccag cgtatggaaa aatccatttt
     2581 cgctgccact ccccagatgc aaaccggtgg ttgccagtgc agatacgccg aacgagaaac
     2641 tccagaatga agcattaaat ggctgggaga gataccatgg catcagacgt agcataaaca
     2701 gcagttgcag cagtccataa ccaaaaagca ttttcgccag cgtatcaccc tcgccgccgt
     2761 tgacgctcaa ccaggcacta caggccacca gcgcaggagc gagctgaatg ccgagtgatg
     2821 tccgcagtgc cgtgggtaat tctcccgaac tgcgcagacg ctgcaagatc acaggttcaa
     2881 ggcttagcca tgagaaaacg cctgcgccta aaaacaccag accggcgtcg gtgtagccca
     2941 acgcaccaca ggccattgcg ctgataaagt tgttggcaac tgtcggcaga tacagtccag
     3001 gcgtggtagc ttcttcaggg tgagatccgc gccataatcc cgcagtttgc caggcggcat
     3061 aagccaactg aaccacgaca ccaaaactga acaggcacac cgccagtggg cgaaaccacg
     3121 gaacaaaacc aatcgccacc agcatcgtcg ttgccggaaa caaactcaca aaactgctca
     3181 gcactggatg gcgcacttct gccagcacgc tatgcggaaa gcgtataagt cgggtaataa
     3241 atgcgctagt taacaatccc cagatgatca tcgccagaat caccagccca tcccctaacc
     3301 agtggctgac ctgccaaacc tggctggcat agcgccaggc aaatcccatc cctatcgtcc
     3361 ccaacacaat accaaagtag cctgccggca aattgagcac tttatcgctc tgcatcttct
     3421 tactcatttt gtttaattta taaagtatat ttgaaatgca ttttatggaa ttttctcgag
     3481 catagctaga gccgcagaat ttgctacggt tatagtaagt cacctgtggg aaaacgctat
     3541 gcgcaaatat cgtctaagtg aagaacagcg agcctttagt tatcaggaag atggcactaa
     3601 aaaaaatgtg ttattacggc agattatcgc cattagcgat tttaacgatg tgatagcagg
     3661 aaccgctggc ggctggatcg atcgcgaaac ggtgttagca caggaaggaa attgctggat
     3721 ttacgaccag aacgctattg cgttcggcgg agcggtgatt tccggtaata cgcgcatcac
     3781 cggcaccagc gtgctgtggg gagaggttta cgcaacggat aatgtctgga tcgataatag
     3841 tgaaatcagc cagggtgcat atataagtga cagcgtcacc attcatgact cactggtttg
     3901 cgggcaatgt agaatatttg gtcacgcctt aattaatcaa cattctatga tcgtcgcagc
     3961 acaaggctta acgcctgacc atcaactcct gctacaaatt tatgatcgcg caagggttag
     4021 cgcctcgcgt attgtccacc aggcacaaat ttatggcgat gccgtcatcc ggtatgcttt
     4081 tattgaacat cgtgccgaag tttttgattt tgccagtatt gaaggtaatg aagagaacaa
     4141 cgtctggcta tgcgattgcg ctaaagtcta cggtcacgcg caggtgaaag ctggcataga
     4201 agaagatgcc attccaacga ttcattacag ttcgcaggtg gcggaatatg ccattgtgga
     4261 aggtaattgt gtgttaaaac atcatgtctt gattggcgga aatgccgtgg tacgtggtgg
     4321 accgattctg ctcgacgagc acgttgtaat acaaggtgag agccgtatta caggtgcggt
     4381 gataattgaa aaccatgtgg agttaaccga ccacgccctc gttgaagcct ttgatggcga
     4441 cacggttcat gtccgtggtc caaaagtcat taacggcgag gggcgcatta cgcgaacgcc
     4501 gctggcaggg ttattatgaa tcgataatac gcgcgtataa gttcacatca tcataggcat
     4561 cattcaggaa ctcagcctgt ttcaggcaac cttcaaggat aaaaccattg cgcaaagcga
     4621 cctggttgct ttgcggattg tccacccgac atttgatcac gaagcgtcta agttcaccag
     4681 actgggcgta atgatgaatc aatgcctgca gcgcctgaga aatgatcccc tgcccctgat
     4741 gagattcgtc cagccagtag cctatttcaa cggttttatt cagtggttca atacgattaa
     4801 acgagataac gccgataagt tcatcttctt tgaaaatcat gaacattttg gcatagccgc
     4861 gttgatgcaa catcacatta ccctgcaccg tttttcgcgt gtcctcttca ctctgaacga
     4921 actgcggcca gtttagcgac tgctgtaacc aggttttatt tttacagatt aactgataaa
     4981 gaggagtgac gtgattttct gcaacagcat gtaattcaag tgattcgctt acttttatcg
     5041 tttcagtcat cttatctcca gtcaatccat gcagttgtta aaaaagccgc ctggctggcg
     5101 gcttaggaaa gtttatacct ctttgacgct ctcctgctgc aggattttga gcatctcatc
     5161 ttttaactgt agcttttgtt ttttcatgcg gaccacttcc gcattgtacc ctcgaccgtc
     5221 ggaaccttcc tttctggcaa tttcatgatc aagtttattg tgtttatcga acaaggacat
     5281 aaagcgagga ttttcgtttt tcagacggga tattaaatct cgatattctg gaaacatacg
     5341 cactccctgt tagtggctga caaggtgtat ctgaaataca caccatcaga ttactcattt
     5401 aacttaggga aaaatgcgag cgggatcgtg tttaaccaga cggcacctcc cgaagaaggt
     5461 gccgaagaaa aaacgattaa ctcatcacgc ggtcgtcaac atactgacgt ttatccggcg
     5521 ctggcgggaa atactgatac agccatgttt cactgatagc gtcgccctga caacgcagat
     5581 acatccgcat atcgaccgga tcagtggagt ccgaagtcgg ataccagtca aactgaatac
     5641 gataaccatc aatgggttca atatagagaa tttcgatttg cttcgcttcc ccactggaaa
     5701 gcgtaatcac cggctcaatg ccttttggtg cggcagcttt cagatcacca ccaacgaaat
     5761 cgacggcaaa acgacgcgcc catttttcgg gatagtgttc acctggtgcc caaccttccg
     5821 ggaaaccgcc catgccggta cgcgtcgcca taacgcgcgc taatgggcaa tgaacaggcg
     5881 gttgcgcact ccagtacaga cgatactgga atgcaaactc atcacccgcc tttacagctt
     5941 tttctggctg ccagaagcag acaatgttat ccagcgtttc gcccgttgtt gggatttcca
     6001 tcaggccgat ggtgccctta ccccacttgt tacgcggttc cacccacaga cttgggcgtt
     6061 tgttatacca gcccataatg tcctgataat gggagaaatc acgatccagt tgcaataaac
     6121 caaacccttt cgggttgttg tctgtgtaag cattgaactg caatttttgc ggattattca
     6181 gcggacggca aatccactcg ccgttgcccc gccacatgga cagacgatca gagtcatgaa
     6241 tttgcggatg aatggtgtcg cacatccgac gttcattagt accgcagctg aacatactgg
     6301 tcatcggcgc aatgcccagc tgtttaatgt ctttgcgcgc atacaggtga ttttccacat
     6361 ccataatcac ctgacttttc tcacaatgga tagtgaactt ataggcacca gtaatgctgg
     6421 cgctatcgag caacgcataa acggtaaagg tagttgcccc cggttttacc gtatcaaacc
     6481 agaaggcggt aaagtcgggg aactcttctt tactgtcggt gtaagtgtca atcgccaggc
     6541 cgcgggccga caaaccgtat tgatatgtgt catcaacggc gcggaaataa ctcgctccga
     6601 ggaatgatac tacatcacgg cgcgccagtt cgggggcttt aaacacgcga aaaccggcaa
     6661 agccgagatc gctttgccct tctaattgtt tggtatcaac acctgcatcg ttgtatttga
     6721 acaactccgg gcgaaagtga atttcacgcg ccagatgtgt tgctggatct acagaaaaca
     6781 tacgaacgcg gcgacggaat cccattccca tatggaagaa ctgagcgtcc agttgacggt
     6841 tctcaacgtt atgccagagc gatttttcgg cgtcgtattg aatactgtta taagcctgcg
     6901 gcgtcattgt cgccagcgtg tcaggtaacg gacgaggcgc accacgccac gctgtttgcg
     6961 ctaagtcgtg cgccattgac tgtagaatgg agaagtcaaa acgctgggtt tgcccgtcgg
     7021 caatatcaga atctgccgcg aatgccgcct gagaaaaaag agaagcaatg ccgctggtac
     7081 cgcacacggc ggccatagcc attgaacctt taataaatcg tctacgatcc atacctgaaa
     7141 gtgagtcctt ctggtgttgt gaatggtccc gcgctggtcg ttttcagcgg caagaaagtg
     7201 cttatttatg accgctcact ctagacaata tttattaata atccaattgt tgaactcaga
     7261 gaatatgcat aaatcggcaa tttttttatg tcgatgggat gagcctgatt cgtttgtaaa
     7321 atcaggcaaa tttttgcaaa ggcggatggt cagaatttaa agcaaaccac agcgacgttt
     7381 actgcgttct tccgcttcct gataaagctg gaactcatcc aaaaccggac aacccaacgc
     7441 ctgctcaaat gcctcgcgac tggcattacc gtgattgcgt gcctgcgttt cattacccag
     7501 atttgactgg aagatccccg ccgcgctaac gggcaagaaa tcttcatagg taatgggttg
     7561 cgccgcgacc caaccacgtt caattaaggg ctgtggatcg tctccgggat gaatcgcctg
     7621 acgatgcgcc tcacctgaag gcgtcagacg gtaccggaac catgccagcc cttgctgacg
     7681 cattaaaaac tcactgtcag gaaaagtgcg gaaggtttcc tgtaaatgca tttggtgagt
     7741 gagattatcc tgcccggttc cagcgttacg cagaagatca tcatacagtt gtcgcccttt
     7801 cggcgttaat gccacgccac gctgctcaat ttcaccaaag cgcgcggtat gcgtgccctg
     7861 tttctgcccc gcaaacaaca ccgtctcttc cagtgcttta aagctggtct ggcgtagtaa
     7921 aatcggtacc tcgcggcgcg gcggcccctc gatgagaatt ttgggttcaa ttccgcattc
     7981 aggcatcatc gactgcaccc ggtcaatatc cagcgtacgt ggcgtcaggt ggttgatatg
     8041 gcatccagga aaacagacca catcagcaat caaccgatgt tcgttgtgca aggcgcgata
     8101 ggtttcttca tctaccgttg ctgactggtg ccagcgaaac gtttccaggg cttcctgcac
     8161 aaactcctgt gcctgtgttt cgttaaaacc gccctgctac tcatattcct ctaacagttg
     8221 tcgacaacgt ggggtgaaga tatcgcgctg acgcagaatc tccgccgctt tctggcgcaa
     8281 aatttcgttc tcgataagct caaggcggag taaggaggta aaaacgcgga agggattacg
     8341 cgccagagaa gcatcatcaa tgggccgaaa tgctgtcgaa tgtaccggca cccctgcctg
     8401 cgagagatcg taatagctca ccgggtacat ccccataatg gcaaacatcc gccgaagagt
     8461 agcaagctct tgtgcagtcc caacgcgaat cgccccatga cgttcaacat tcagtcgcgc
     8521 cagctcgtct gcatttacca ttttttcgtg cagttgagga ttgttttcca gcacagccag
     8581 attcacatca gctaccagtt ccagcagcgt gccatattgc ggaacttctt gctggtacat
     8641 ggctgacatt gcctgcgaaa actgttcccg aatctcatcc gccgtgatgc tgttcgccat
     8701 agtgtctgcc tccagtggtc agtaatctgg agtgtaggta accgcattca ctctggcggg
     8761 aagaatttac aaactgtgat ctcgccgcga aaacatcaat attatccatt ttgctgtaac
     8821 ataattgctt taattgttaa taatattttg caatcaagtt atcataatca aacaacttca
     8881 cttgtcagcg acaccgcttc gtttttaaca tcgcttatgg aaaaaaatag tctgtttagt
     8941 cagcgcatcc gtttgcgcca ccttcataca ttcgtagctg tcgcacaaca aggaactttg
     9001 gggcgcgcgg ctgaaaccct taatttgagt caacctgcgc tctctaagac attgaatgaa
     9061 ctggagcagc tgactggcgc tcgcttgttt gagcgtggtc gtcagggggc gcaacttacc
     9121 ttacccggcg aacaattttt aacgcatgca gtcagagttc ttgacgccat caacactgcc
     9181 ggacagtcgc ttcatcgtaa agaagatctt aataatgatg tcgtcagggt tggtgcacta
     9241 cctactgcgg cactggggat attaccttcg gttataggtc agtttcatca gcaacaaaaa
     9301 gagacgacct tgcaagttgc gacaatgagt aaccctatga ttctggcggg tttaaaaacc
     9361 ggggaaatcg atatcggcat tggtcggatg tcagatcctg aactgatgac cgggcttaat
     9421 tacgaactgc tgtttcttga atcgctgaag ctggttgtcc gccctaatca cccgctactt
     9481 caggagaacg taacgctaag ccgggtgctg gaatggccgg tcgttgtatc accagaaggc
     9541 actgcgccac gccagcattc agatgcatta gtgcagagcc agggctgtaa aattccttcg
     9601 ggttgtatcg aaacgctgtc tgcttcgcta tctcgtcaac ttacggttga atacgactac
     9661 gtgtggtttg tcccttctgg cgcggtaaaa gacgacctgc gtcatgccac gctggtggcc
     9721 ctgcctgttc cgggacatgg tgcaggcgaa ccgattggaa tactgacccg cgtagatgcg
     9781 acgttctctt ctggttgcca gttgatgatt aacgctattc gaaaatcaat gccgttctga
     9841 aaggtgaagg gatctgtcga tccctccttg aacattttca caccgtagcg aaactaactg
     9901 gttcacccgc tccgcgaggt tctgccgaca cagaatgttt gttcagacgg aatacgtcca
     9961 ccgcctcagt taatcgtgcc gcctgttctt caagggacac cgccgctgct gaggcctctt
    10021 ctaccagaga agcattctgt tgcgtcacct tatccatttc agaaatcgcc tggctaacct
    10081 gcgttatgcc tctactttgt tcatccgagg cggcggcgat ttcctgcatg atatgtgtga
    10141 cactcgcgac ggcatcaaca atagtgctca tggttttccc tgccgttgcc acctcatccg
    10201 accccaggtc aattaacctg actgattcac tgatcaagcc ttcaatctct ttcgccgctt
    10261 gggcgctgcg gcttgcgagt gtccgtactt cgctggcgac aacggcaaat ccacggcctt
    10321 gctcacccgc tcgcgcggct tcaacggcag cattcagtgc cagaatattc gtctggaaag
    10381 caatactgtt gatgacggcg gtgatctcag aaattttctt cgaactcgtg gagatagcgc
    10441 ccatcgtttt tactacaccg gaaaccgtct gcccgccatc gctggcttta atagaagcct
    10501 cttgcgccag tttgctggca tgatgcgcgt tatccgcatt ctgtttcacc gtcgcagtga
    10561 gttgctccat gctggcggca gtttgttcga ttgccgccgc ttgttcttcg gtgcgagatg
    10621 acaggtccgc attgccagct gaaatttcgc tggtgccacg ataaatctct tccgcaccct
    10681 gtcgaacagt ccctactgtc atccccagtg aatgctgcat ttgctgtaaa tgacgattta
    10741 agcgaccgat ttcattacga cccgccggtt catccttcat cgtcagatcg ccactagcga
    10801 ttttttcaat ccgttgtgcg gcatgttgca gtggacgaat gacgatccga cgtagcacca
    10861 taaatgttat cagcgtcatg accagggcaa gcacaaacgc gccaatcatg aacatcccac
    10921 ccaggcgggt gcgctgatgg gccagttccg ccagttggtt ggccctggtg ctacgtatct
    10981 taacggcttt gttcaaaata tcggtataag cattatccag cggtcggatc tgctcacttt
    11041 catgattaat aatcgcttca aacatgccat ttttggcata tttcaacata ggttgcatac
    11101 ccgtgatata agcctgaaag cgttgattta attcagtgtc gagggcttca tcagcaggtg
    11161 ttttcaccgg tcgattctga taagcacgat aaccttgctg cgactgttta atctccgatt
    11221 cggcttgcgc aatattgcgc ttcattgctt ccatttccgc aatacgactc gcagccccgg
    11281 cctgaatcat attgatccgg gctgaacgca aaaaatctga actgttcgct aacccggtcc
    11341 gaatctcaat ctccgcagta acatcacgct gatcgcgatc ggcctgccag aggaaatagc
    11401 cagccagagc agaacttaat gcgaatagaa ctaagatacc gccaagaatg caggcaaata
    11461 acggaaccag cctgatgtga tgcaaaaaac ctaatcgatg tgagggagtt gtattcatta
    11521 ccgttgtctc tcgtccaggt ttactcctga tagaaagtca tcggcacttg acggtaatta
    11581 cttatgacca aaaacctctt tgcgtcacat ttttacaaca attattgcag agaaaattaa
    11641 tgctattgga atgaagagag taacgaatgg ctaaactggc taacccgaat gacgaaaaat
    11701 tctggaaaac agcacgcaat ttcgtgaagt gattattttc gtccctaagg aaacctcata
    11761 ggatgcctgc gggtgcctga gactttctgt tttcaggcta accgggaatc agaaagacga
    11821 aggccccgac cgatatttct atcaaccgag gccaacgaat gaacctagca attcagatct
    11881 tagcctctta cccgccatcc ggcaaggaga aaggctatga agcacaaccc tctggtggtg
    11941 tgtctgctca ttatctgcat tacgattctg acattcacac tcctgacccg acaaacgctc
    12001 tacgaactgc ggttccggga cggtgataag gaggttgctg cgctcatggc ctgcacgtcc
    12061 aggtaagggc aagcgcgggg attttccccg cgcatctgac tagcagattc attttctcat
    12121 ggcacccctt ttacacttca gttagtgcat gtttttttcg aggcactaat agaaccatca
    12181 ttacagacaa atttgccgcc tgccgtacag tgtgagatcc cccctttcga accagaacag
    12241 ggtttgcgcc cggcatcggc ataaaaggaa acaagcgttc ccataaacaa aataagtgcc
    12301 agttttttca tataacctcc atggtttatc gaatcttatg ttcgatttga acgttatacg
    12361 ctggtttatt ttgataccgt tgatgagatc aaagatcggg tgaattgatt aaaaataagc
    12421 cagatggacg tatgggcaag gattatggtt ttatttgtca tacaaataaa tataataggc
    12481 gcttccgatg tagacccgta ttcttcgcct gtaccacggg tcggttttag tacaggcgtt
    12541 ttctttaaat atccttcagg aacgtttacg cggcatcatg cgtagaagtg tgttgtcctt
    12601 ccagaaatag tggtgtgcca gtgcagccgc agcgtgcaac ccgatgacaa aatatcccag
    12661 attcgccagc gtctcatgcc acgactttaa gctatcaacc cgttcgaaat tggcctctga
    12721 agcgtaaggc atcgtcaaac caaacgcaaa ccacgggttg ccccggttat acatcatcac
    12781 caaaccaatc actggcagcg caataaacag gagataaatc accaaatgtc ccaaatgcgc
    12841 cagtcccgtc atcatcggtt ttggcttagg tataatcggc ggggttgggt atttcagtct
    12901 taacaaaaga cgaaccacca tcagcacgag aattgagata ccacaggaaa catgaaccat
    12961 gttgatgagt ggccgatcgc tacgtgggaa gaaaccacga aactccattg cgcaatacgc
    13021 tgcgataacc agcaaaaaga ccagccagtg aatgctgatt tgtaaccttg agtatttatt
    13081 ttccataaca tttcctgctt taacataatt ttccgttaac ataacgggct tttctcaaaa
    13141 tttcattaaa tattgttcac ccgttttcag tacgttacgt ttcactgtaa gaatggagat
    13201 tgcccatccc acaatcctgg gctaagcctg gaaaggatca attttcatcc gaacgttcct
    13261 gacaggagaa aaccatgagt aaagttggta ttaacggttt tggtcgtatc ggtcgactgg
    13321 tgttgcgtcg attacttgaa gtcaaaagca acatagacgt tgtcgctatt aatgatctca
    13381 cttccccaaa aattctcgcc tatctgctga aacatgattc aaactatggt ccgttcccct
    13441 ggagcgttga ttttacggaa gattcactta tcgttgatgg gaaaagtatc tcggtttacg
    13501 ccgaaaaaga ggctaaaaat attccgtgga aagcgaaagg ggcagaaatc attgtcgaat
    13561 gtactggctt ttatacctcc gccgagaaat cgcaggcgca tcttgatgct ggtgcgaaga
    13621 aggtgttgat ttccgcccct gccggtgaaa tgaaaactat cgtttataac gtcaatgacg
    13681 acactctgga tggcaacgac accattgttt ccgtggcgtc atgcaccact aactgtcttg
    13741 cgccgatggc caaagccttg catgacagtt tcgggataga agtcggcacg atgacgacca
    13801 ttcatgccta tactggcacc cagtcactgg tggatggccc acgtggtaaa gatttacgtg
    13861 cttcacgcgc agcggcagaa aatatcattc cccacactac gggggcggca aaagccattg
    13921 gtctggtgat cccggaactg agcggcaaac tgaaaggtca tgcgcaacgc gtgccggtga
    13981 aaacaggttc ggtcactgaa ctggtatcga ttctcggaaa aaaagtgact gccgaagagg
    14041 tgaataacgc acttaaacaa gcaaccacca ataacgagtc atttggttat accgatgaag
    14101 aaatagtctc ttccgatatc attggcagcc atttcggttc ggtgtttgat gccacgcaaa
    14161 cggaaattac cgccgtgggc gatttacaac tggtgaaaac ggtcgcctgg tacgataacg
    14221 aatatggctt cgtcacgcag cttattcgca ccctcgaaaa attcgctaaa ctgtgacgcg
    14281 cacaggcgga ggaaaaaacc tccgcctctt ttactcatta cgactgtaaa taaaccacct
    14341 gggtctgcag atattcatgc aggccatgtt taccatctgc gccgccaata ccggatttac
    14401 gccatccggc gtggaagcct tgcatagctt cgaagttttc acggttgatg taagtttcac
    14461 caaacttcag ccctttaatg gctttcatcg cgacgttcag attttgggta tagattgatg
    14521 aggtcaggcc gtaatcactg tcattagcca ttgagatagc ctcttccagc gtgtcaaatg
    14581 cgaccaccgg cagaaccggg ccaaaggttt cctcatgcat aatcgacatt tcctggcgaa
    14641 catccagcag caatgtcggc ggataataat atcctttccc ctctaccgct ttgccaccca
    14701 acgccactct cgccccttct tctactgcgc gcgccacttt ttgctcaacc ctttccagcg
    14761 ccgcggcgtt aatcaacggc cccatcgcaa tgtcgttgcg ttcagcgggg ttaccaaatt
    14821 gaaccgcctg catcgcttca cccagccgat tgacgaactg atcataaatg cctttctgta
    14881 cataaacacg ttctgcacag ttacacactt gcccactatt aatgacgcgt gaatcaacga
    14941 tggctttgac tgccagttca agatcggcat cgtccattac gatagctggt gctttacccc
    15001 ccaattccag acacactttg gtgatgtttt tcgccgcagt cgccatgatc ttctcacctg
    15061 cagagacgct gcctgtcata ctgaccattg cgacctttgg gttacccgcc agttcttgcc
    15121 caacggtttc accacgcccc agtacaaggt taaacacgcc gcgcggaagg cctatttcat
    15181 cgacgatttt ggcgaatgca atcgcattgt ttggcgtaaa ttcactaggt ttaatgacga
    15241 tggtattacc ggtcaaaaga gcgggagcca ttttgcgggc aatgaggaag aacgggaagt
    15301 tccacggcag aatgccggta gtcacaccaa gcgcacgttt aaacaaaaga atattttctc
    15361 ctggacgatc gctttgaata atctcgccct cgtaacgccg tgcccactcc gccatgtaat
    15421 cgatatagtc ggcagtaaaa gccacttcga cttcagccag ctgctggatc ttgccccctt
    15481 cttcaacaat cagcgcactg atttcactgg cgcgttcgcg gatcccggcg gagattttgc
    15541 gcaaccaact ggcgcgttca atagcaggca acgcttccca ttctggttgt gcacgttctg
    15601 ctgcatcgat tgccttacgg gcatcctcgg cctgaccatc gggtatgcgg gaaatgacag
    15661 cctctgtagc agggtttacc acatcaatcc atgcgtctcc acgccaggta acaaactgtc
    15721 catcgatata cataggatgt tgaacgggta ctgacatggg cgactcctgt gatttatatg
    15781 tttgttttcg gtgaatgcat tgttaattaa catcaatcat cagagagacg gcatcgtgaa
    15841 aggacaattc tgtgaagggc ttcataaaaa caagtgtaaa aggaataaaa ggcagaaata
    15901 aaagtcagga gtcatgcccg tttgcaaaca agtggttcac aaacgggcaa tttttaacgt
    15961 aacgagcgac tttccatcgc ctcgatgagc accgcatcat gtttcagcgt ttgccatgca
    16021 tggatgactt ctgccggaaa atcaacgtga acgataaaat ctcgcccgcg gggaccgtag
    16081 ccatcgctat catcgcgtaa acgcggcagc tcgccagtga gtagtgagag ataacgctca
    16141 actggccata atccttgtaa ctgattaata aagattacac tgtctgcgtt atttcctaac
    16201 tgaggaacga atccgggata acttaaccag cgtggtgcat cgggattgtc cccagtcata
    16261 cggcggaacg tcgccatcgt gcgccgctgc atggtgggct cctgaacaac gatagccgta
    16321 tgaactcgtt ctacggcctg attcaatagc gcgatgctaa agcgtgcgtt ttcaccgcag
    16381 tttgttgact ggtcttcaat ccagattttt tcatgcggaa tgtgccagaa ctgatgagcg
    16441 atatccgcca ggatggtcgc ttctgctctg ccagtggtgc ggatagtgtt gtagtgcgga
    16501 tgctgtgcga tggcgctata caaaaaagtt gtcgagtgac cgataccacc actaatcagt
    16561 aaaggaattt gctgatcgcg agcaatctta catgccgcgt cgatagtcgg cataaccgca
    16621 ttgcctgcaa ggatcacgca atcggcctga tacggcacct caccggagaa atcatcctgc
    16681 gccagccact gtccgataac atttatggca tctatagttg ccggcgaaag cgtcggaaac
    16741 ggggtaatgt tcatggttcc tccttatcgt tcccttaaga atactgcgtg agataatagt
    16801 gagaagcgga gcactctgaa aggaggaagt cgtatttcct taaccgccac tccgcaagcc
    16861 tgacatatct tttaaggaaa tgctgtagat aaacattatt catcttgcag cagagaattg
    16921 tatttttctg aattcctgaa tttttaccct taccgtttta gaattaataa aaaacccggc
    16981 gctaaatgct taccgggttt tttctttatc aggcaaatag cagtgttaac cgctaatctg
    17041 ctccatcgcc tgcaaaatac gcttatccga aataggataa ggcgtaccaa gttgttgagc
    17101 gaagtaacta acgcgcaact cttctatcat ccaacggatc tctttcacgt cttcatcctc
    17161 acgacgtgcg ggcggcagtt tgttgatcca ttgctgccac gcctgctgga cgttttcgac
    17221 tttcagcatc tgcgcacggt cgcgatgtgg atcaaccgcc agtttttcca gccgtttttc
    17281 aatcgcctgc aaatatcgca gcgtgtcgcc cagccgtttg aagccgttac cagtgacaaa
    17341 accgcgatat accaacccgc ccatctgcgc tttaatgtca gaaagcccca gcgccatggt
    17401 catatccacc cgccctttca gacgtttgtt gatattgaac actgccgtaa ggatttgctc
    17461 gacctgcttc gcaatatcca ccaccgtgtc gttcagttcg gcacgcactt tttcatgcag
    17521 cgcagcaaag ccttcttccg tccagaccgg gccaccattg gcgtcgatca atttatccac
    17581 accgcaggag atacagtcgt cgatcagctc cagcactttg ccatacgggt taaagtacag
    17641 tcccagcttg gctttgttcg gtaacttttc atgtaaatat ttgattggcg atggaatatt
    17701 cagcagcagt agacggcgaa gaccgttcca cattgcctgc ttttgctcca gcgggttatc
    17761 aaacagtttg atcgccacac tgtcgcgctc atccaccagc gccggccacg ccttcacttt
    17821 gtagttgcca cgcttctgtt cgtagctttc cggcagctga ccaaaactcc agatatgtaa
    17881 gccgctctgc tcgataccgt catccgccac cgcggacagc gtttcctgca ctttgccttt
    17941 cagcgcatct ttcagatcct gtaatgagcg cccttctttt agcttcttat ttttgtcgtc
    18001 caccacgcgg aaggtgattt tcaggtgatc gggcacctga tcccagtgcc agtcttcgcg
    18061 gtcaacggta acgccggtca tccgccgtaa ctcgcgctca aggctgtcga gcaacggtaa
    18121 ctccagcggt ttgacgcggc ctaaaaacgc ttcggcatag tttggcgcgg gtacaaaatt
    18181 acggcgtacc ggtttcggca acgatttaat cagagcaatc accagttcgc ggcgcagacc
    18241 ggggatctgc cattcaaacc cgctttcctc aacctggtta agtaacggca gcggaatatg
    18301 tacggtcaca ccgtcagcat ccgcgccggg ctcaaactga tagctcaaac gcagcttgag
    18361 attgccctga tgccagaagt tcgggtaatc cagcttgctg attttttctg cgccctcttt
    18421 gatcaacatg cttttttcaa agttgagcaa atcaggcgtt tcgcggctga cttttttcca
    18481 ccagctatcg aagtggcgcg cagagatcac atcgtggctg atgcgctggt cgtagaactc
    18541 aaacaacgtt tcgtcatcaa ccagaatatc gcggcgacgt gatttgtgtt ccagctcttc
    18601 cacttcggcc cgcagtttca ggttttcacg gaagaatgcg tgacgcgtct gccagtcacc
    18661 ttccaccagc gcatggcgaa taaagagttc acgacataac gccggatcga tctggctgta
    18721 gttgaccttg cgcgcggcaa caatcggcaa accataaaca gtgacttttt ccgttgccat
    18781 caccgcgccc tgcgcccgtt cccagtgcgg ttcgctgtag gtacgtttaa tcaaatgctg
    18841 agcgacaggc tccacccatt ccgggtcgat acgcgcagca atgcgccccc acaggcggct
    18901 ggtttctacc agttccgcca ccattaccca tttcggcggt tttttgaata aaccagaacc
    18961 ggggaagatg gagaaacgcg cgttacgtgc gccggtatat tcttgtttat cggcatcttt
    19021 catgccgata tgggaaagta aacccgtcag caacgcaatg tgaatttcgc gatactccgc
    19081 cggttcgctg ttaaccggaa tgccaagttc tttcaccacc tgacgcaact gggtgtagat
    19141 atcctgccat tcgcgcacgc gcagatagtt gaggtaatcg gtacgacaca ggcgacggaa
    19201 ggcgttggaa gaaagcgcct tttgctgctc gccaagataa ttccacagat ttacaaacgc
    19261 gaggaagtcg gattctttgt cgtggaagcg acgatgtttt tcgtccgatg cctgctgttt
    19321 gtccatcggc cgctcacgcg ggtcctgaat ggagagcgcg gacgtgataa tcatcgcctc
    19381 acgcacgcag ccatgtttct gcgcttccag caccatacgc gccagacgtg ggtcgacagg
    19441 caactgcgag agctggcgac cgagcggcgt cagtttatag gcgctggcct gttcatccgt
    19501 ggtgatcgcg cccagttctt cgagcagacg cacgccatcc tggatattgc gtttatccgg
    19561 tgcttcgaca aacgggaacg cagcgatatc gcccagcccc agcgcggtca tctgcaaaat
    19621 aaccgaggcc aggttggtac gcagaatctc cggatcggta aactccgggc gcgaaaggaa
    19681 atcgtcttcg gaatagagac gaatacagat cccttcggac acacgcccac agcggccttt
    19741 acgctgattg gcagacgcct gcgaaatggg ttcaatcggc aaacgctgca ctttggtgcg
    19801 atagctgtag cggctgatac gtgctgtacc ggggtcgata acgtatttaa tccccggtac
    19861 ggtcagcgag gtttccgcga cgttggtcgc cagcacaatg cgccgtccgc tgtgcgactg
    19921 gaacacgcgg ttctgttcgc tgttcgaaag ccgcgcataa agcggcaaga tttcggtatg
    19981 acgcagattt agcttgttca gcgcatcggc ggtatcgcga atttcccgct caccactcat
    20041 aaagatcaga atgtcgccag ggctttcctg actcagttcg tctacggcgt caaaaatcgc
    20101 ctgcaactga tcgcgctcgg tgtcgtcggc ttcttcaaca atcggtcgat agcgcacttc
    20161 caccggatag gtccgaccgg agacttcgat aatcggcgca ttattaaagt ggcgcgaaaa
    20221 gcgttccggg tcgatagtcg cggaagtgat aatgattttt aggtcaggac gccgcggcag
    20281 caactctttc aaatagccga gcagaaaatc gatattcagg ctgcgttcgt gtgcttcgtc
    20341 aataatgatg gtgtcgtact gcatcagcag gcggtcttgc tggatctccg ccagcaggat
    20401 accgtcggtc atcagcttga ccatcgtgtt atcactaacg tgatcgctga aacgcacttt
    20461 gtaaccgatg caaccgcccg gctccgtttt cagctcttcc gcaatacggt tcgccaccgt
    20521 tcttgccgcc aggcgacgcg gctgggtatg gccgatcagc cctttaatcc cgcgccccag
    20581 ctccatacag attttcggta actgagtcgt tttaccagaa cccgtttccc cggcgacgat
    20641 caccacctgg tgatcacgaa tcgcttcgag aatgtcctgt tttttctgac taacaggtaa
    20701 attgtcagga taagtaattt ccggtcgtgc cgcttcacgc agcaggactt tccctgccgc
    20761 ttggtcaatc tctttcgcca tctcctggaa aatggcctgt tgtgcatcag gatttttaac
    20821 cttcttaaca ccgtgcagac gccgagaaaa acgcagtctg tcgcgcagca tcagcgaatc
    20881 cagccgctgc tgcaaggccg taaaggtcaa tttttgttgt tctgtcataa cgttagtggg
    20941 cagtagtctg cctgattaat tttcttttaa atgatagata tagagtacca catcgcggct
    21001 ttctatgcgt tgttcaataa attcgaacat aggcttcgat ttattgcgct atctctgtgg
    21061 caggattgtg aataaagtgt cagcaagcaa cggggcatcg cccattcaaa catctataag
    21121 gaaacaccat gagcaaggta ttagttctta aatccagcat cctggcaggg tactctcagt
    21181 ctaatcagtt gtccgattat tttgttgaac aatggcgcga aaagcactcc gctgatgaaa
    21241 tcaccgttcg cgacctggct gcaaatccga ttccggtact ggatggcgaa ctggttggtg
    21301 cactgcgtcc gagcgatgcg ccgctgactc cgcgtcagca ggaagctctg gcgctttccg
    21361 atgagctgat tgccgagctg aaagcccacg acgttatcgt tattgcggca ccgatgtata
    21421 acttcaacat ctcaactcag ttgaaaaact attttgacct ggttgcacgc gcaggcgtta
    21481 ctttccgcta taccgagaac ggtccggaag gtctggtaac gggtaaaaaa gccatcgtta
    21541 ttaccagccg cggtgggatc cacaaagatg gaccaacgga cctggtgacg ccgtatctgt
    21601 ccacgttcct cggctttatc ggcattaccg atgtgaaatt tgtcttcgcc gaagggatcg
    21661 catacggtcc ggaaatggca gcgaaagcgc agtctgacgc gaaagcggcc atcgacagca
    21721 ttgttgctgc ataaccggac acgatcttga tgtggactgt agttcagtcc acatctcaat
    21781 ccacttacct gttttcccat aatttcagca tcgctttgtg atcttcctta agcacaatat
    21841 gcgaacgtct ggctcgaata taactaatcg cttcatcaac ggttttacag tgtccgtaac
    21901 ataacaacca tgccgccact accagcgcac tgcgcgataa tcccaacgcg caatggacca
    21961 gaacgctgcc ttgctcttcg cgtaatgttt ccagcatcgc cacggcctgt cggagctccc
    22021 cctcttccgg aaccaccaga tccagcatcg gtacacagaa atagagtcga tcttttgtcg
    22081 ctcgtccccg agggaattca aaggtgacgt ccagaaccgc attctgtgcc ggaatatgtc
    22141 gtggaaacgc ccctaaataa acaccagcag taattttgct caccggctcc aggcgacgac
    22201 aaaaccaacg catagacagc cacatcccga tgcgccaggg caatgtcagc cagtaaacgg
    22261 cgggcgggag tttcccctga ctatctttcc ctgttgttat cgcgccaagc ccaccgtacc
    22321 cacggccaat aatgagtagc gataatactg gccaacataa ccagactgac caccataact
    22381 gaatcatcac cattagctcc atcaacacaa tgcacgagcc cgcgcctacg acatatggca
    22441 gtgctatttt gattcgacgt tgatcaggtt tctgataatt ccaacgacga tcgacgggca
    22501 ccatccattc aatcaacata cctaccgcca gccctgtgat gacatcaata aaatgatgct
    22561 gccaggtcgt cagagtagaa atagcgatga gtaaaaacca tccgccacag actttacgcc
    22621 acctctcagc cagatgctga cgaaagtgac gccaaagtag ccagcagaga ataatatgca
    22681 gcgaaggaga ctggttataa ggcagatcaa acagttcaag ttgcgaaaat agccatcccg
    22741 tcaccccact cacttcagga cggataaaac taaacttcag cggatagagc aaaaaaccgc
    22801 agcaggccat taccgttgcc agaataagcc ggtggacaag tcggcgctgt tcgaatgtcg
    22861 tgctacaaac gaacagcgaa aatccatata aaagatccag actccagtaa ggaacaatag
    22921 tccagggaag aaaagggatc gccgtttccc aaccgaatac ctgactgggg atatcatggc
    22981 tgttaaggtc ctgaaccgcg gtgaactgat taagagatcc ataggtgaag aagaaaaacg
    23041 gggccagcaa caataaccag ccagcgcctt gtagcaatac gttccgacgt tcagttatca
    23101 cgacgcaccg ccatcgaaac cgtaaaaata ccccactcat caatccgttg tgtgcattta
    23161 tcaaatccgg catcacgcac gagtgaatcc atctcccctt gcgaacgcac gcgcattacc
    23221 cacggtttac catctttatg actggttaac accccggcaa tcatctccag ttgagggtgc
    23281 cacggctgcc cggtgtagat gagaatgccg cccggttcga tggcattggc aagacctgcg
    23341 agtgagtttt ttacctgctc attttcggga aaaagctcat acaggccaga gacaatcgcc
    23401 agcgtaggcc gcggagttaa cgcgctgagt tcctccgggt taaacgcatc gccctgttca
    23461 aaacgcaccc gcccagacat tccccgttgg gcaatcatcg cttgcccctg tgcaacattt
    23521 aactcactgt aatcacgtaa caaaatatcg cttacggcag gctcgtttgc cagcgcatcc
    23581 agtacatagc gcccatgccc tgcggcaatg tcaaccacgc ggacggctaa acctttggcg
    23641 tggagatcgg caacggcttg tttaatcagt atttgtaaat gggttttacg ctggcgaata
    23701 ccgcgccagc caacactgtt caggtagatt ttgtcaatta agcgcccgaa tgcgttactg
    23761 ccttgcggtt gattttgata gacataatcc agggaactgc ctgaatcaaa gccggtgctc
    23821 attccgagat gcaggcccgc agagtgcgcc ccgaacaatt tcatcgcctt tcgcataaag
    23881 cgatacgcca aatcaaccgg cgataatggc acgggtccac ctgaaaggag ccgccagcga
    23941 tccgctgatg gtccagtgcg gtcttcatgc tgataatcaa atttttgtga cttgttagca
    24001 tataaacgac taataaagct ttgcattttt tcaaatgcct gcgccctgtt ctcttcaccc
    24061 aacgtgtcgt gataaaagcc tggcagcaga tgcagctctt tcagagggct acgtaatctc
    24121 tgataaaaat caatctgcgg ttggcgatgc accacatagt catcgcctga tatcagaagt
    24181 tgcgtgggga gcgtaatcgc cgccgcatct ctaataatac gttcagacgt tttgtagaga
    24241 tcgagcaaga tattaacggc aatcgcccgt gtgatcagcg gatcattatt gaaactcgcc
    24301 acccgttgcc gatcgtgggt caaatagcgt cctttcacat aggaattaat aaaaaacaga
    24361 ccacgcagac gatgccataa cgccagcgca ggacgtgcca gcggtacata caatttaacc
    24421 ttaaaggccg gagaagccag caccagcccg cgaattgcag gcgcataatc atgaacccat
    24481 gtggcaacca gcactgcgcc gacgctttgc gcgatcacta ccacctcatc cagtccgacc
    24541 tggctatcgc tggcagcaaa acgaacaaat tcatcgacat cctgcactga acgcgcaaga
    24601 gatgggctat aaccacgcgg ccccgaagtt tgtccatgcc ctcgggcatc ccaggcataa
    24661 aaaacagtat ctggcatcgc cagttcatca acgagatgtt gtagacgacc agaatgttca
    24721 tgaccgcgat gaaataagac gatgaccttt ttagccccgg gctgtaaagc gggccagtgc
    24781 cgataaaaca acgctgtatt atcactgctg gtaaaaaaat gttccccatg gatgcgtgaa
    24841 ttttccattt tctctatttt ccttcagtaa cagcagtagc gaataaaata aaaaaatacc
    24901 ggagcggtga aaattaatga gtcaatcctg tcaaggagtc cgccatgtcc tggtaatagt
    24961 ttcccactat ctttaacacc aatatctcgt ttgatggctg acatcacgac atcgccgcaa
    25021 aaaccactaa taccaattaa caaacccgct aataatgcct gtaatgtatt tagcggtgtc
    25081 agcaacggtc caataatcag tgacgcaatc atggtggtga ttacgccccc caccagacct
    25141 tccagcgttt ttcccgggct gactttgggg accacttttc ttctgccgca ggatttcccc
    25201 cataaatact gtgcaatatc gtttgactcg gttaatgcca aaagaaataa caccagtaac
    25261 gccccgccct ggatatttgt ggttggcaac attaataacc aggcggcatg actcaaggcg
    25321 aaaacggtca ttatccagcc ccaaaaaatg gcgctcacgg tatgcagaaa accggagggg
    25381 tcccccataa aaacttgccc ggtggctaat attagaaacc cagccaaagg tataaataat
    25441 agatacagct caaagcaatt aaaaccgata aacaaatagt taagaggaat aacccaatat
    25501 aaccaccgcg gaaaatgtac agatataagc gtacaatatt ctttcaatgc cagaaaactt
    25561 atcagcgcaa aaaacgtcaa cgtcatccag cgtggacctg aaatcaccag tgaaaaacaa
    25621 atgacgatgc cccaccaggt acgaatacgt aatgtaactt catcctcttt tctctccggt
    25681 aaccgccata gcagaaaatg gtttatcagc gttgctaaaa ttaacaacgc gaaaagtgtt
    25741 gccagagatt tttccagcat tatctttcag cgctcataag aagcacactc cgacaacgat
    25801 taatcgcagt ccagagaagc agaattgaag caatgctcca taaaagatta ttccactgca
    25861 tccactgtgg ataaatggca acggccagac cccacagacc aaatattaac gcgcgatcgc
    25921 ttttgccaaa tggaccggca taactgcgaa caccgttaat cgtctgcgcg agtaaaccgc
    25981 aaaactcggt caatatggtg caaaataaca tgagtatcac gagtgatgcg ttactttccg
    26041 gtaaaaataa aaatggtaaa tagagcgcaa tatcggaaat aacatcacca gtctcattca
    26101 aaattgcccc aagccgtgtt tgctgattgc actcacgcgc caacatgcca tccagcgcat
    26161 tgagcgccat acggataaaa agcacgatgg gcaatagcat aaagaggatg ggttgtgcca
    26221 ccaacatcaa cagcaatccg gtaagaagag aaagcgccag tgcagcaaga gtgatgtgat
    26281 tcgctgtaac gtggtgcttg taaagccaaa acatcgtcgg ccttaacagc gactgaaaga
    26341 gcggttttat ctggtagagc gtcattatca atccttgata attcggtttg cgctatggtg
    26401 catcaattcc cctgacggag atatcgcaac acgccgcttc attaaaaatt cagaatatat
    26461 caaggtattg gggactcact gtcaggaata attataccca attaatcaaa gggttaagaa
    26521 atattacgtt gtggatcaat acaactcatc aagttttctc atcgcttgtt cccgtattgc
    26581 gcgcgtctta ccgttaagcg ccagctggcg gactaaatcc agttcagggt taacaagctc
    26641 taacgccagc aaaggatcac gtttcagcct cgcctttaat acatcaactg gaaaacgggg
    26701 attattcatc gccacaatcc accactcggg atcaatttct gccgccagta ctgcaggcgg
    26761 tgtgtggcgg ttgttaagtg cgccgaactt gaaaaatgtg agattgcttt tcgccagtgt
    26821 actccaccat atacgatccg gttgttttgc cagtacatcc agttgtgcta acgcatcggg
    26881 gtcgagtgca taatagacgc gtaatgcttc atcaacctgc gcacgggagc tgttttcctg
    26941 cctcagaaaa aacttcgccc aaatatcatt gcgtggcgta ttttgctgta attccttgag
    27001 gacaacagcg ggtaaattat cccaactaac taatgcactc tctaaaaagt cccagtcttc
    27061 caaacgtatc agggcggtgg catttaatag catctgatca tcaacttcac cgtggctgat
    27121 aagctcagac aaaatcatat cagcgctggt ttcatacgca gctgaatctg ctttcggatt
    27181 atttttccga tgcgataaag cacgttgcca aattttttgt ttcatcgacg tgctcagata
    27241 ctgatttttc gccagattac tccacccctc aggatcgttg aaacgtgtca ataaccgttg
    27301 tagagcagcg tcagagtgaa ttacctgcat atcccgacag caaaagtagg caggggattt
    27361 tttctccatc cttttaacaa aattaaactg taagttatct ggcaatgcta aaaataacac
    27421 acgtattaga tcatcgtcat tctcgaattt ttggtataaa taggctgcaa ctttgtcact
    27481 ctcttgtggc gtcatccgca ataattgacc atggtgtttc atctctgcca gcttgaacgc
    27541 taagcttgtg acaacattgt ttgatgaatc gttcgccaac tgcattgcct gattgtaggt
    27601 gagttgtcct ccagcaccga cctgacgaat agaggaatca tcactgcgga tatagccatc
    27661 aataatggat tcaggcaata accacgcatc tttataaagc tcctgaatga tcatatccca
    27721 atctcttttc gccagagtca ttacgatatc cagagggaga ttttcacgta gcgcaagatt
    27781 caacggaccg acaccatcag gtagacgact cattcgcgtc attatttcga cagagagcgg
    27841 ttcacggtcg agcaacccca gaacaacagg ctggtaaccc aattgctgat cggtcaactc
    27901 cagaatctca ttgtcaggca tatatctcgc catactttca atcgtttgtt catcattgct
    27961 attaataagt tgtttgcgta ggttagctga aattccttta agccttggga gagttcgcat
    28021 tatggcaaga tcaaatgtcg caatatctcg tccttcaaat gcagcaacgt tgccttcatc
    28081 cagcatttgc aattcctgca ctaactcagg agaaggctta gggcgaaaca gcgcttcttt
    28141 tttcatctga ttacagacat tccctgccat gcgaatattg cttcgcttaa aaatttcttt
    28201 cagagcatct tcatcattta ttagacccca ataatatgca tcaggattta ttatcatcag
    28261 cgcttttttg acattttcag tgtcattttt gtcactcgca cggaccatga attcactatt
    28321 tgtcgaagac gcaacaacag gtgcaattat gtcattgata aatattccac gcgcattaat
    28381 actcccctta atcgaagacg gattatcgta atatgagtcg ataaatacca gtccacttgc
    28441 atttatcgaa ccggtaatta acagtggtga atccgtaact atattcgttg ccgtcacgtt
    28501 accgagaata acccatggat tagaaagaga gaaagtggaa atattacctt tgactgtcaa
    28561 attaccaaga acaatcaatt tttgattact ctctatatta ccttctacca ctaaatcgcc
    28621 acgatgcact ttccagtccg atgcagataa accgaagggg acaagattca cgctactgtt
    28681 gatagcttgt tcaacttgtt gtttatcttc agcggtagcc gcagaaaaaa atggttcaat
    28741 atcaactgta tttgcaacac atcgaagttc cgcctgtact ggaagactca gcataaacag
    28801 gggaagtaaa cctatccagc cgcaaatcct ggccttaaac atatgcactc catgccatta
    28861 aaaaagaaac aaaagggaaa tgcatctttc ccttttgtat attcgttatt ctcgcgaaat
    28921 accatccaac gtagacaaca cttcctcaga aagatgcaat ttttcagcag ccatattctc
    28981 ccgtaaatgc gcaacagaag acgtccctgg gatcagcaaa atattcggtg aacgttgtaa
    29041 cagccacacc agcgccacct gcatgggtgt tgcacccagg ctcgcagcaa catccgacag
    29101 cgtagaggat tgcagcggtg taaagccccc gagcgggaag aacggcacgt aggcaatgcc
    29161 gtcacgagcc aaagcatcaa tcattgcgtc atcagcacga tgagcaatgt tgtattcgtt
    29221 ctgcacacag acgatttcgg caatcttgcg cgcctctgca acctgcgtcg gtgtgacgtt
    29281 gctcaggcca atatgtttta ccaggccttg ttgttgcatc tctgccagca cggtcaggct
    29341 ggcttcaatc gatccttccg ctgggccatg accatccccc ataataacgc gcaggttaac
    29401 cacatccagt acgtccaggc cgagattacg tagattatcg tgcaccgctt tttgcaactc
    29461 tgccggagaa aaggcgggca accaggaagc gtcctctcca cgccgcgcac caattttagt
    29521 gacaattgtc aggtcgtcag agtaaggata aagcgcttcg cggataatct gattggtgac
    29581 gtgcggacca taaaagtcgc tggtatcaat gtgattgacg cccaatgcca gcgcctcgcg
    29641 cagcacggtt atagcgacgt ggcgatctct tggggggcca aaaaccccag gacctgccag
    29701 ttgcatcgcg ccataaccaa gacggttaac ggattttgta ccgagagtaa atgtattgct
    29761 gctcatcgca ccctgccctg taataaaacg gattaagaaa aatcagtata gaaacgaaaa
    29821 gagataaata atgcgagggc gtaaacaaga ttactggatg agttgctcta ttgtcctgaa
    29881 aaacacaaaa caaaacgcgt aaagcgccgc atccggcaat aaaaaaaccg caacctgtat
    29941 cagcgttgcg gtttaggtat cacatgctaa aaattagaat gttcagtatc tggacgttac
    30001 tgtttcggtc gcagattctc atcaaatacc agacgtttcc tgttcggttc aacttcccgc
    30061 aatggctcaa cttgatgcaa cgtctgctta caacgagtca ccagcacctg atactcatgc
    30121 gtaccctgct ttttccatgc cagctcctgc tcactgagtt cacgaatcgc tttcgccggg
    30181 ctgccgacaa tcaggtaatt ggcaggcatt tctgctttgg ctttcacaaa tgcggatgcg
    30241 ccaacaatgc tgttctcgcc aatcaccgcg ccgtccatca ctaccgcgtt cattcccact
    30301 aatgcattgc ggcggataat gcaaccatga aggatagcgc tatgaccaat atgtccatct
    30361 tcttccacga cagtatcctg ctcgggaaaa ccgtgcataa cgcaattatc ctgaatgttc
    30421 gcgccatctt tcaccacgat acgaccaaaa tcgccacgca ggctggcatt tggcccaacg
    30481 taaacgccct tgcctagaat aacatcgccg atcaataccg ctgtcggatg gacaaaactc
    30541 tcttctggca caaccggagt cagaccatct atctggtaaa ttggcatatc gcctcctgtt
    30601 caatattcaa accgccaaaa cgttgaaaat acaggcttcc cggcgcaggc agttcaccga
    30661 ccgaggtttc acctttttca ctaacgaacg ctaaagcagc aggcgctacg cgctgataaa
    30721 tgttgataca cagctgacgc gccgtatgcc ctgcccagtg cgccggaagc aactcctctg
    30781 gcaacaatgg gtctttaagg acgacacggc gataaaaatg gatcagtaaa agctgaatat
    30841 gaaatgcacg ctccggggtt aactcgtctg ctgcctcttt taacagcggc accagcgggc
    30901 ggaatgactg aataaaggtt tcgtacatgg cattttgttc agttaaatgc cagcactctt
    30961 ccactctggc acgcagtgct gcacgagaaa gcgccagtgg tatttgcgct tcaaaacaaa
    31021 tcacgttatc cgccaccccc gcttcatgca aaagtgtctg tacatccgcc agtttttgcg
    31081 acggcgatgc catcaggctg ggtgccagtg cgccaaaacc ttgccagatc aactgctttt
    31141 tgacatcagc cagcgttgat ttatctaaac cttccgagag caacaggagc catttaccat
    31201 cccatgcagg ttgctctgcg cgataaattt tactttctgc ccgtcgcgtc aggcgcaagc
    31261 ctttatcact gaggctatag aaactgcgtc gcccgatgcg ggaaacatcc agccagcctt
    31321 ctttattaag acgaaacaaa gcggtgcgca cgaaacgctc accaaatccc agcccttcca
    31381 gcaaagcagc cagactaccc agccagattt caccaccacg atgggaaagc gaatcaccat
    31441 acagagagga gatcaaagat gtgccactga ccggaacagc gtttacagca tgttggataa
    31501 aagtaacaag tttactcatt cgattcagtc tgttgttatt gttgtgattt atgaatcata
    31561 gcacaggtcg tatcgacttc tggccctggc gctttacctc accagggcca gacgacacgt
    31621 caggcaccaa caatattgcg cagatcaaac acccgacacg ccttgccttc tgaacgcggg
    31681 atactgccac agttaacgat catcacatcg gtagagatcc ccaccatcga cttaatccga
    31741 tggcgcagct ggtggcacac ctggcaacgc tgctcatgcg tcaatgtcag gctgctttct
    31801 ttcaactcca ctttcacaga aagtgaatca agatgccccc ggcggttcac ctccagttgg
    31861 taatggggcg ataaatgttc gaatttgaca atctcttctt ccagttgtga cgggaagaca
    31921 tttacaccgc gaatgatcaa catgtcatcg ctgcgtccgc tgatgcgatc catgcggcgc
    31981 atagtccgcg ccgttcctgg taacagtcgg gttaaatcac gcgtgcggta acgaatgacc
    32041 ggcaatgctt ctttggtcag cgtggtgaat aacagttcgc catgttcacc atcggcaagc
    32101 ggtgtgccgt catgaggatt aacaatttca ggatagaaat gatcttccca aatggttggg
    32161 ccgtcggtag tttccagaca ctccatcgcc acccccggcc ccatcacttc tgacaggcca
    32221 taaatatcca gtgcggtgat ccccagacgc cgttcaatct ctttacgcat ggcctgtgtc
    32281 cacggctcag caccaaacac tccaacccgc agcgagcaac cgctggcatc accgcccaac
    32341 tgccgctcca gctcttcaat caggttaagg caataagatg gcgtaaccat gatcatatct
    32401 ggttgaaaat cgcggatcag ttgcgcttgt ttttccgtct gcccaccaga catcgggatc
    32461 accgtagcgc ccagacgttc ggcaccatag tgcgcgccca gcccaccagt aaacaggccg
    32521 taaccatagg caacatgaat tttgtctttg ggcgtgccac ctgccgcacg caaggaacgc
    32581 gctacaatat tggcccagtt atcaatatcg ttttgcgtat agccgacaac tgtcggttta
    32641 cctgtggttc ccgaagaagc atgaatgcgc accacttgtt ccatcggcac ggcaaaggtg
    32701 tcgaagggat agttgtcgcg cagatcctgt ttggtggtac agggaaattt acgcagatct
    32761 gaaagttccc tgaaatcatc aggatgtacg cctgctgcgt caaatttgca ccgatacatc
    32821 gggacatttt catacgcgtg tttgagcgtc catttcagac gttgtgtttg caacgcctgt
    32881 aactcatcaa cagacgcggt ttcaatcggg tcaagctttg tattggttat cattgtaggg
    32941 tactcgcagg ttgatatgct caaacacgct ccagaatcat ggcgatgccc tgaccgacac
    33001 cgatgcacat ggtgcacaat gcgtaacgac cgttacgccg atgcagctca tggctggcag
    33061 ccagtgccag gcgggcacca ctcattccca acggatggcc taaggcgata gcgcctccgt
    33121 tggggttaac atgtggggca tcatcaggca gccccaattc gcgtagtaca cccaacgcct
    33181 gggccgcgaa cgcttcgttc agttcaatca cgtccatatc gtgaatactc agccccgcgc
    33241 gttccagcac ccggcgagtt gcaggcaccg gaccaagccc catcaggcgc ggttccaccc
    33301 cggcggttgc catggctacg attcgcgccc gcggtgtcag tccttgcgct gctgccatct
    33361 gttcactggc aataatcaac gcagcggctc cgtcattcac cccggaggca ttgcctgcgg
    33421 taatcacccc attggcacga aatggtgctt ttaacccacg taactgttcc agcgtcgttt
    33481 ccgggcgcag atgctcatca tgttgtattt ctgttacaac gcctttcttg tttttcaaca
    33541 caaccggaac aatctcctca gccagaatgc ctgaggattg cgcttttgcc gtacgttgct
    33601 gactgcgtag cgcaaaacta tcttgatctt ctcgcgatat ttttaacagt tcagctacat
    33661 tctctgccgt ttccggcatg ctgtcagttc caaattgttg agccatgagc gggttcacaa
    33721 atcgccagcc aatagtggta tcaaacatct cagcctgacg agaaaatgca ctggttgcct
    33781 tgcccataac aaacggtgcc cgagacattg actccacgcc accagcgatc agcaaatcgc
    33841 catcgcccgc tttaatcgcc cgtgcggcga accccagtgc gtccagcccg gaaccacaca
    33901 agcggttaat ggttgtgccg gaaacactct gcggcagccc cgccagtaac gtcgccatcc
    33961 gggctacgtt acggttgtct tctcccgcct gattagcaca gccgaggatc acatcatcga
    34021 tacactccgc atcgagacgc gggtttcgca ccagcagctc ccgcaaaggg atagcagcca
    34081 gatcgtcagc ccgaacacct gataatgccc caccatagcg gccaattggc gtacgaattc
    34141 cgtcacagat aaaggcttca cgcatcaggc ttctcctgta atggtgccgc cgatgcggtg
    34201 agatttaccg cgaaacagcg caaccgtttt ttgttgttgg ttaacaattt cgatgtcgta
    34261 aacaccggtt tgcttgccct ggtgacgtac ctgcgcagta gcagttaagg tgtctccggc
    34321 aaagcctgga cgcaaaaaat caatcgtgca ggcagaagcg acggctgcca gcccctggct
    34381 attgcaggcg taggcaaagg cagtatcagc cagtgaaaat agctgcccgc cgtggcaact
    34441 ttgatgaccg ttaagcattt gtgcagtgac ggtcatggtc actacagcaa agccttcatc
    34501 cattgagata atgtcgatgc caagcgcttt ggcgcaggca tcgttctcat acattgcatg
    34561 ggcattttgc caggccttat gactcataac cgctctccag aagcgctcgt tggcgcagca
    34621 atgaacatgg gcgatagcgt tcttcgccat agtgatgttg tagattttcc aggagcctta
    34681 atattcgctg ccagccaagt tgcgctcccc aggcaagcgg gccatatgga taattcaccc
    34741 caagacgcat ggcggtatcg atatcctgtt cagaggccac gcctttttgc agcgcatcaa
    34801 gggcttcatt gatgatcatt gctaccgttc gccaaatcag cattcctggg taatctgcaa
    34861 tttgcagcac tgttttgccc tgctgttgca ggtaataaat ggccttgcgg gtcgctgagt
    34921 ccgggttcac cgctgccgca gcaatggtca ccaccttgcc cgccattttg tcgaccacta
    34981 ccaccgggcg tgccagtcgt attgccagcg cctgtgccgt ctcgccttgt gtctcaatca
    35041 ataaaacatc gtcaatttcc gtgacgccgt cacttttctt ttctactttc attgggctaa
    35101 aactgtcgct taccgcttcc aggccaacaa ctgcctcacg ttccgcgcgc cagtcgtaca
    35161 cgcccagccc acttttcttg cccaaccgtc caccaatcac cagttcctgt tgcaccagcg
    35221 aaggtaaaaa acgacgctcc tgccagaaag cgttaaacac cgaacaggtg acagcaaaat
    35281 tgacgtcctg accaatcaga tcggttaatt ccagcggccc catcgggaaa ccagcgccat
    35341 cgcgaagtgc agcgtcaatc acttctggtg cagcaacctg ctcttccagt gcccgccagg
    35401 cctcggaata ataaggacgc gcaacacggt taacgataaa tccgggggtc gaatgacagc
    35461 gcactggctg cttaccccaa ctcaatgtca gttcacacaa ctgctcgaca acttccgctg
    35521 ccgttgccag cccactgacc acctccacca acttcatcac cggtgccggg ttaaaaaaat
    35581 gcagccccgc aacacgttca ggatttttta cctccgcagc aatcgcggtt atagagattg
    35641 acgaagtgtt agtggtcaat agcgtttgtg gcgggcaaac ttccgccagt tgtgcaaaga
    35701 gcgctttttt aacttccaga cgttcagacg ccgcttcaat aaccagattc gctgctgcca
    35761 gcgcgtgaat atcggtcacc gggatcaggc gtttcaatgt gcgttcacag gtttcagcgg
    35821 tcagttttcc ccgcgtcacg cgtgaattta gccgcgcgtg tatcccgtcg attgcgcggg
    35881 tcagcgcttc agcagaaatg tcatacagta aaacctgatg tccatgactg gcggcaactt
    35941 cagcaatgcc tgcccccatg gtgccgctcc caatcactgc cacagtttgc acatttatca
    36001 tcatgctatt tccccgtgaa ctgcggtgag cgtttagcca ggaacgcact gacaccttca
    36061 cgataatctg cgctccgtcc ggcaagtcgc tgatagtcac gttccagatc cagttgcgta
    36121 tcgagcgtat tggtttcagc gctatttatc gcttgcttga taagtccaag accaaatgtc
    36181 ggttgtgtcg ccagatgccg tgccagctgt tgcgcggtat ctgccagcgt ttcatcatca
    36241 acaacctgcc agatcatccc ccattcgtgc gcctgttcag cactcagttg attccccagc
    36301 agtgccagcc ccatggcgcg cgctcgccca gcaacgcgtg gcagtaacca ggttccaccg
    36361 caatcgggta ctaaacctaa cttactaaac gccatgacga attttgctga acgggcagca
    36421 ataacgatgt cgcatcccag tgccagtgtt gcgccagccc ctgccgccac gccattgact
    36481 gcacagatca ccggttttgg cagttttgcc aggcgacgta ccagtgggtt atagaaacgt
    36541 tcaactgaca tccctaaatc cggtgcgggg cctgtgggat caacgttacg atcgttaaga
    36601 tcctgaccag cacaaaatcc gcgcccggca cctgtaagta acaggcaacg gatagtgtcg
    36661 tcgcgctcga cctgtttcag gcactctgcc agttgtgcgt gcatctcatc attaaaactg
    36721 ttcaggcgtt ccgggcggtt gagcgttagt gtcatcacgc ccttttctac atgactgagg
    36781 atgaattcca tcattagcgt cctttaaagt cgggcgtgcg tttttgcaag aaagcggaga
    36841 tgccttcatg acgatcttct gttgccgcca gcaaggtgaa taactgtcgc tcctgggcaa
    36901 gtccggcttg caaagccact tcctgcgact ggcgcagcgc ttgctttgcc gcttgtaagg
    36961 ccagcggcga gtgacgtgcc attttcgacg ccagctgtaa ggcgtattcg agggttaaat
    37021 cgctggggaa gacgtcgcta accagcccgg cctgctgtgc ttgctgagcg gtgatacttt
    37081 ctccgctcag caccattttg ctggctaacg atttaccggc actacggatt aaacgttgcg
    37141 ttcctcctgc gcctggcatg atgccgagag tgatttccgg caaaccaaaa cgcgcgttct
    37201 ctccggcaac caccacatcg cacaacaatg ccagttcgca acccgcacca agcgcgtaac
    37261 cgttgacggc tgcgatgaga ggtttgttga aggcctgcaa tcgcgcccat agctgcgggc
    37321 gtgtatcgtt taaggtggcc gcgagatctt tttctgccat ttcgttgaga tcggccccag
    37381 cggcaaaaaa gcgtgcatta ccggtaatca cacagaccga aatgctgcta tcggtagccg
    37441 cagcttccag ttcatttacc agttgcgtca gaagggcatt atttagcgca ttacgtgcgg
    37501 cgggacggtt aagggtcagc aacaatactt gttgctgacg gctgacgatc agttcgctca
    37561 tgccatcccc ttcgcgtcaa agtcaaccac cacatcgctg gtcagcggca gtgcctggca
    37621 actcaacaca tagcctgcgg ccagttcatc cggttccaga ctgtaattgg tttccatcgc
    37681 cactttgcca cgcagcactt tgcatttgca ggtcgcacag acgccgcctt tgcaggcata
    37741 gggcagatca gccccctggc gcaatgccgc atcgagaatg ctttcatcgt cggcattaag
    37801 cacgatttcc cgatcccgcc catcctgacg tacagtcact ttttgtccgt cactttgcac
    37861 gttaacgcta cgtttgacgc gcgtgccagg cgtattaaac cgctccagat gaatggtttt
    37921 atctggcatt cccagtgctt ttaaggcggc ttccgtctca tccatcatcg ccgccggacc
    37981 acaaataaat gcctcatcat aaagacgaaa attaatgagc gaggccccaa gtgactgtaa
    38041 tttttcaccg tcaatacgcc cgtgaagcag atcgctgtcg agggtttcct gactgaaaat
    38101 gcacaacaac tgtaaacgct gaggatattt gtctttcagg tctgccagtg cctggcgaaa
    38161 catcatgctc tggctggtac ggttaccgta gatcagggtg aactgacttt caggctcggt
    38221 ttgtaaagtg gtggcgataa tcgccagcat tggcgtaata cctgatcctg ctgcaatagc
    38281 cagatagcgg ccctggcgtt cggcctgtgg ctgatagccg aaatgcccct gcggcaccat
    38341 gacctccagc gtcataccct ggcggatgtg ttcgcgggca tagcgggaga aacgtccgcc
    38401 ttcaatggct ttcaccgcca cactaatttc gccaggcaga tagctgcggc aaatggagta
    38461 acaacggcgt aattcttcac catcaaagct ggcttttaag gtcaaatgtt gaccggggcg
    38521 aaagcgatac gcctcctgca aaggctgggg caccgcaaag gtaatggtca ccgcatcacg
    38581 ggtttccggc tccacttttg ccaccgttaa ggaatgaaac gttgtcatgg catcctcaaa
    38641 tacatttgaa ataatcgaaa ggttcgcggc aactatcgca gcgatacaat gctttgcagg
    38701 ccgtggaacc aaattcactg ataagtgtgg tatggacgct ggcgcagcgc gggcaacgta
    38761 cttctggcgg caaatgggca tggcaactgt gtccggcggg cgggctaatg ccatactgtc
    38821 gcagacgctc acgggcatcg ggggtcatcc agtcggtggt ccatgccggg tcgagttgca
    38881 gcacaacctg aacgggggta aacccgtggg ttgtcattgc ctcacgtatc gcgccaatca
    38941 aatgttccgt tgccggacaa ccggaataag tcggcgtaaa gccgatcacc catccttctc
    39001 ccatctgtgt cacattacgc accatgccta aatcggtaat ggtcagcacc gggatctccg
    39061 ggtccgggat ctggctgagt agcgcccata tctcatgaac ctgtggcggt gcaatggtgg
    39121 ccagacgttg catacccatc tcctctgtta ccattgctga ccgggcaaga cacactggag
    39181 atactgcatt tctgccagca tgggtccaag gtgttcggta tgcagtcctt ttttaccgcc
    39241 agtgcgatac gcctgctctt gcggtacgtt caatgtggct tcgttgattc cggcaaaaac
    39301 ttcggcttcc cacgctgcgc gtaaagtgcg tggatcaacc gcaataccct cttcactcag
    39361 tgcaatatca atctcgtcgg catcgaacag ttcggcagta aaacgccaca acttgtcgat
    39421 ggcctgctgc atcttttgcc ctgatacgtc agtaccattg cccagtcgct ccagccagcc
    39481 acgactaaaa cgcaggtgat aacgcgcttc tttaattgct ttggcagaaa tcgccgccag
    39541 ttgcggatca cggctttcca tcagacgggt aaagagcgcc acatgccatg catcgatgaa
    39601 atactggcgt gcaatggtgt cggcaaaatt gccgtttggt tgttcaacca acaataagtt
    39661 gctgaactgg cgctcgtctc gggtgaaggc cagggtatct tcatcacctt ctcccgctaa
    39721 ttcagcggca tacgataaga agttacgtgc ctgacctaat aaatcgaggc caatgtttgc
    39781 cagtgcgaga tcgatttcca gttccggtgc gtgaccgcac cattccccca accgctggga
    39841 gagcaccaga cagttatcgc ccaggcgcaa ggtgtaagcc gttaactgat tcatttccga
    39901 cctcacatgt gctcaatgcc atcagggatg gtgtaaaacg ttggatggcg atagaccttg
    39961 ctttcagccg gatcaaaaaa ttcaccgcgt tcttccggtt gcgaggcaac aatttcactc
    40021 gccttcacca cccaaattga acatccttcg ctacgacggg tgtaagcatc acgggcattt
    40081 tccagtgcca tccgctcatc ggcagcatgt aaactgccaa catggcggtg tgataagccc
    40141 tgtttgccac gcacgaaaac ttcgtataac ggccagtaaa cattactcat tttgaatctc
    40201 cttatgcgac cttacgggca agttgttttt gtgcatgggc cagcgctgct tcccgtaccc
    40261 aggtaccttc ttcccaggct ttacgtttgg cgtcgagcct ctcctgatta caaattccgc
    40321 gaccgttaat tacttcgtta aactcctgcc agtcgatctc accaaagcgg tagtgaccgc
    40381 tttcagtgtc aaaatgcaga tccgggtcag gaacggtcat accgagcatt tcaacctgtg
    40441 gaatggtgtt atccacgaaa cgctggcgga gttcgtcgtt ggtgaaacgt ttgattttcc
    40501 aggcgagact tctggcgctg tttggcgagt tatcatcgtt tggcccgaac atcattaagg
    40561 ctggccacca gaaacggtta atggcgtctt gcaacatctg cttttgagct tcgctgccct
    40621 gcgcgagtgc catgcaggct tcaaacccct gacgctggtg aaaactctct tctttgcaga
    40681 ttttcaccat tgctctggca tacgggccat aagaagtgcg acataacgcc acctgattga
    40741 cgatcgctgc gccatccacc agccagccga taacgccgat atcagcccaa ctcaatgtcg
    40801 gataattaaa gatggaggaa tatttcatcc gtccgtcgag cattttttgg tagatgtctt
    40861 ctctggcgca gcccagtgtt tcggcggcgc tatagagata cagaccatga ccggcttcat
    40921 cctgcacttt ggccaacaga atggctttac gccgcaaggt tggtgcgcga gtgatccagt
    40981 taccttcagg caacatacca acaatctcgg agtgcgcatg ctgcccaatc tggcggatca
    41041 gtgtctttcg gtaagcatcg ggcatccagt cctgtggctc gatagccgtt tcctgggcta
    41101 tccgttgctc aaagcgttct tcttgggtca cgtcatcacc tttacgattc cagttttcgt
    41161 gaattaatta aaaacacgaa tcacattgtt ttgtgaaagt tacacaatgg ttaactaaaa
    41221 tcacaaattt tgtatgcttg aactgtgacg gatttcgcac cctattttta taaatcatta
    41281 tttatcagtg cattaatttt ttccgcagtt gctatacaga tcgcatagtt aacatttcgt
    41341 taaaagaacc cttgcttttt atgattcgcg atttaactat tagcaacaga aatgtgaaac
    41401 atctggagag tagcgatgca gcagttagcc agtttcttat ccggtacctg gcagtctggc
    41461 cggggccgta gccgtttgat tcaccacgct attagcggcg aggcgttatg ggaagtgacc
    41521 agtgaaggtc ttgatatggc ggctgcccgc cagtttgcca ttgaaaaagg tgcccccgcc
    41581 cttcgcgcta tgacctttat cgaacgtgcg gcgatgctta aagcggtcgc taaacatctg
    41641 ctgagtgaaa aagagcgctt ctatgctctt tctgcgcaaa caggcgcaac gcgggcagac
    41701 agttgggttg atattgaagg tggcattggg acgttattta cttacgccag cctcggtagc
    41761 cgggagctgc ctgacgatac gctgtggccg gaagatgaat tgatcccctt atcgaaagaa
    41821 ggtggatttg ccgcgcgcca tgtactgacc tcaaagtcag gcgtggcagt gcatattaac
    41881 gcctttaact tcccctgctg gggaatgctg gaaaagctgg caccaacgtg gctgggcgga
    41941 atgccagcca tcatcaaacc agctaccgcg acggcccaac tgactcaggc gatggtgaaa
    42001 tcaattgtcg atagtggtct tgttcccgaa ggcgcaatta gtctgatctg cggtagtgcg
    42061 ggcgaccttt tggatcatct ggacagccag gatgtggtga ctttcacggg gtcagcggcg
    42121 accggacaga tgctgcgagt tcagccaaat atcgtcgcca aatctatccc cttcactatg
    42181 gaagctgatt ccctgaactg ctgcgtactg ggcgaagatg tcaccccgga tcaaccggag
    42241 tttgcgctgt ttattcgtga agttgtgcgt gagatgacca caaaagccgg gcaaaaatgt
    42301 acggcaatcc ggcggattat tgtgccgcag gcattggtta atgctgtcag tgatgctctg
    42361 gttgcgcgat tacagaaagt cgtggtcggt gatcctgctc aggaaggcgt gaaaatgggc
    42421 gcactggtaa atgctgagca gcgtgccgat gtgcaggaaa aagtgaacat attgctggct
    42481 gcaggatgcg agattcgcct cggtggtcag gcggatttat ctgctgcggg tgccttcttc
    42541 ccgccaacct tattgtactg tccgcagccg gatgaaacac cggcggtaca tgcaacagaa
    42601 gcctttggcc ctgtcgcaac gctgatgcca gcacaaaacc agcgacatgc tctgcaactg
    42661 gcttgtgcag gcggcggtag ccttgcggga acgctggtga cggctgatcc gcaaattgcg
    42721 cgtcagttta ttaccgacgc ggcacgtacg catgggcgaa ttcagatcct caatgaggaa
    42781 tcggcaaaag aatccaccgg gcatggctcc ccactgccac aactgttaca tggtgggcct
    42841 ggtcgcgcag gaggcggtga agaattaggt ggtttacgag cggtgaaaca ttacatgcag
    42901 cgaaccgctg ttcagggtag tccgacgatg cttgccgcta tcagtaaaca gtgggtgcgc
    42961 ggtgcgaaag tcgaagaaga tcgtattcat ccgttccgca aatattttga ggagctgcaa
    43021 ccaggcgaca gcctgttgac tccccgccgc acaatgacag aggccgatat tgttaacttt
    43081 gcttgcctca gcggcgatca tttctatgcg catatggata agattgctgc tgccgaatct
    43141 attttcggtg agcgtgtggt gcatgggtat tttgtgcttt ctgcggctgc gggtctgttt
    43201 gtcgatgccg gtgtcggtcc ggtcattgct aattatgggc tggaaaactt gcgctttatc
    43261 gaacccgtaa agccaggcga taccatccag gtgcgtctca cctgtaagcg caagacgctg
    43321 aaaaaacagc gtagcgcaga agaaaaacca acaggtgtgg tggaatgggc ggtagaggta
    43381 ttcaatcagc atcaaacccc ggtggcgctg tattcaattc tgacgctggt ggccaggcag
    43441 cacggtgatt ttgtcgatta atcggtgaat gaagggcaac ggcgaatagt tgccctttta
    43501 tttcaccaaa ttttgtgacg ttgtcacgtt atgcatgatg tgtatctgta ttttcagggc
    43561 acccactgta tgaaaagctg gcacacctgc caaacaacct ggcaggtgca ggcaatcccc
    43621 tttacatcag tactgataat gtgaacctga ctaaaccgcc cacagagcgc ggttgctaac
    43681 aagaacacaa catctgacga ggttaataat gggaagcccc tctctgtatt ctgcccgtaa
    43741 aacagccctg gcgttggcag tcgccttaag tttcgcctgg caagcgccgg tatttgccca
    43801 cggtggtgaa gcgcatatgg tgccaatgga taaaacgctt aaagaatttg gtgccgatgt
    43861 gcagtgggac gactacgccc agatctttac cctgattaaa gatggcgcgt acgtgaaagt
    43921 gaagcctggt gcgcaaacag caattgttaa tggtcagtct ctggcactgc aagtaccggt
    43981 agtgatgaaa gacaataaag cctgggtttc tgacaccttt attaacgatg ttttccagtc
    44041 cgggctggat caaacctttc aggtagaaaa gcgccctcac ccacttaatg cgctaactgc
    44101 ggacgaaatt aaacaggccg ttgaaattgt taaagcttcc gcggacttca aacccaatac
    44161 ccgttttact gagatctccc tgctaccgcc agataaagaa gctgtctggg cgtttgcgct
    44221 ggaaaacaaa ccggttgacc agccacgcaa agccgacgtc attatgctcg acggcaaaca
    44281 tatcatcgaa gcggtggtgg atctgcaaaa caacaaactg ctctcctggc aacccattaa
    44341 agacgcccac ggtatggtgt tgctggatga tttcgccagt gtgcagaaca ttattaacaa
    44401 cagtgaagaa tttgccgctg ccgtgaagaa gcgcggtatt actgatgcga agaaagtgat
    44461 taccacgccg ctgaccgtag gttatttcga tggtaaagat ggcctgaaac aagatgcccg
    44521 gttgctcaaa gtcatcagct atcttgatgt cggtgatggc aactactggg cgcatcccat
    44581 cgaaaacctg gtggcggtcg ttgatttaga acagaaaaaa atcgttaaga ttgaagaagg
    44641 tgcggtagtt ccggtgccaa tgaccgcacg cccatttgat ggccgtgacc gcgttgctcc
    44701 ggcagttaag cctatgcaaa tcattgagcc tgaaggtaaa aattacacca ttactggcga
    44761 tatgattcac tggcggaact gggattttca cctcagcatg aactcgcgcg tcggacctat
    44821 gatctccacc gtgacttata acgacaatgg caccaaacgc aaagtcatgt acgaaggttc
    44881 tctcggcggc atgattgtgc cttacggtga tcctgatatt ggctggtact ttaaagcgta
    44941 tctggactct ggtgactacg gtatgggcac gctaacctcg ccaattgcac gtggcaaaga
    45001 tgcaccctct aacgcagtgc tcctgaatga aaccatcgcc gactacactg gcgtaccgat
    45061 ggagatccct cgtgctatcg cggtatttga acgttatgcc gggccggagt ataagcatca
    45121 ggaaatgggc cagcccaacg tcagtaccga acgccgggag ttggtggtgc gctggatcag
    45181 tacagtgggt aactatgact acatttttga ctggatcttc catgaaaacg gcactattgg
    45241 cattgatgcc ggtgctacgg gcatcgaagc ggtgaaaggt gttaaagcga aaaccatgca
    45301 cgatgagacg gcgaaagatg acacgcgcta cggcacgctt atcgatcaca atatcgtggg
    45361 tactacacac caacatattt ataatttccg cctcgatctg gatgtagatg gcgagaataa
    45421 cagcctggtg gcgatggacc cggtggtaaa accgaatact gccggtggcc cacgcaccag
    45481 taccatgcaa gttaatcagt acaacatcgg caatgaacag gatgccgcgc agaagtttga
    45541 tccgggcacg attcgtctgt tgagtaaccc gaacaaagag aaccgcatgg gcaatccagt
    45601 ttcctatcaa attattcctt atgcaggtgg tactcacccg gtagcaaaag gtgcccagtt
    45661 cgcgccggac gagtggatct atcatcgttt aagctttatg gacaagcagc tttgggtaac
    45721 gcgttatcat cctggcgagc gtttcccgga aggcaaatat ccgaaccgtt ctactcatga
    45781 caccggtctt ggacaataca gtaaagataa cgagtcgctg gacaacaccg acgccgttgt
    45841 ctggatgacc accggcacca cacatgtggc ccgcgccgaa gagtggccga ttatgccgac
    45901 cgaatgggta catactctgc ttaaaccatg gaacttcttt gacgaaacgc caacgctagg
    45961 ggcgctgaag aaagataagt gattgtttca gacaaaaaaa cggcaccagg tgccgttttt
    46021 tacttatgag cgaaccagat taataccgta cacacaccga cttagtttca caccaaccgt
    46081 ccagccagtc ggggccaaaa tcacggcccg ttcctgactg cttcatccca ccaaacggta
    46141 agttagcgtc aattaaggta tggctgttta cccacaccgt ccctgcctgt aagcgatcgc
    46201 tatattccag agcctgggag agattttgcg tccagacact ggcagttaag ccatattccg
    46261 tgtcgtttgc cagttgtaac gcctcttctc catccgctac tcgcaccagg tttaccaccg
    46321 gaccaaacac ctcttcacga gttaagcgca atttagcatc gggatttacc accagcgttg
    46381 gcgcaacata atacccctct ccggctggtc cattcgaccc gcgaatcagc tctgcttgct
    46441 gtgcctgcgc atcgtcgagg aatgaacaca ctttgtcgca gtgcgcacga gaaaccaaag
    46501 ggttaatctg tgcaacaggt gacatccccg gtcccacttg caacgatttt accgcctgct
    46561 caaatccact aaccagcgtg tcaaacaacg gcgcttcaat ataaattcgc gaactggcgg
    46621 cgcatacttg cccttgattc aggaagcttc cggtcatcaa gccttcaata acccattgcg
    46681 gatcagcatc ttttaataca attgccgggt ttttaccgcc cagttccagc gttacacgcg
    46741 ttaagtgatc agcagcagtt ctggcaatac cttttcccgt cgcggttgaa ccggtaaaac
    46801 tgattttcgc aacatgagga tgtgacgtca gggccgcgcc gcatacagca cctgacccgg
    46861 tgacgacatt aaaaacgcca tcagggatac cagcctcgct ggccagttcc gccacgcgca
    46921 acatcgtcag tggcgtggtt tccgaaggct taatcacgat tgaacagcct gctgccagtg
    46981 ctggcatcac cttccacata ccaatcatca acggaaagtt ccatggcaca attcccgcca
    47041 ctacgccaac cggctcttta cgcgtccagg cctgataacg cgccccctgg ggtaagggaa
    47101 tcgacaagtc cagcgtttta cccgcgattt tggtcgttaa cccggcggta taacgcatcc
    47161 agttcagcgt acagcccact tcaaaagcac gggaaatggc aattgacttg ccttgctcca
    47221 gggtttccag ttgcgccagc tcctcactgt gctgttccac cagatcggca aaacgtagca
    47281 gaatacgttc acgctctgcg ggtaatcgcc cggcccagcg acgcgagaca aaggcccgcc
    47341 aggcagacat gactgcgtta tctacatccg cttcgttggc atcagcagta gacgcaattt
    47401 cttgcccggt ggccggatca aagatcgcca atcgtttttc actttgtgcg gggcctggac
    47461 gaccatcaat ataaagaccg tgttgacgat cgagaaactg ttggacctgg cttaatactg
    47521 ctacatgcgg ctctgtcatc acttttcctt attatttacc cagtgtgatt tcagtgtacg
    47581 cagcgacaag aaaagtcact tttttcgctg tgacattcgc tttgtcagct gtgacaagct
    47641 ccgcaaatcg tgacaataac cgcttatgtc tctatgcctg agaagactta tttcctatag
    47701 tcaggctcat tagcttcgtt tactgcaaca caaatgcaac aataaaaata catttcacag
    47761 agcgaaaacg tgccatgaac cccgcagtgg ataatgagtt tcagcaatgg ctttcccaaa
    47821 tcaatcaggt atgcggaaat tttaccggac gcctgctgac tgagcgttac acgggtgtac
    47881 tggacacgca ttttgccaaa ggactaaagc tgagtaccgt gacaaccagc ggggtgaatt
    47941 tatcccgcac ctggcaggaa gtaaaaggca gcgacgatgc ctggttttac accgtttttc
    48001 agcttagtgg tcaggcaata atggagcagg atgagcgtca ggtgcagatt ggcgctggcg
    48061 atattacgtt actcgatgcc tcacgcccct gttcgcttta ctggcaggag tcttctaaac
    48121 agatttcatt acttttgcca cgcactctgc tggaacaata ttttccccat caaaaacctg
    48181 tctgcgcaga aagactggac gctgacttac ccatggtgca actcagtcat cgcctgttac
    48241 aggagagcat gaataatccg gcactttctg aaacagaaag tgaagctgcg ctacaggcga
    48301 tggtgtgtct gctgcgcccg gtacttcatc agcgggaatc tgttcaacct cgtcgtgaac
    48361 gtcagtttca aaaagtggtt acgttgatag acgataatat tcgcgaagag atattacgcc
    48421 cggagtggat agccggagag acaggtatgt cagtacgtag tttgtaccga atgtttgccg
    48481 ataaaggttt ggtagtcgcg caatatattc gtaaccgtcg tctcgatttt tgtgcagatg
    48541 cgattcgcca tgccgcagat gatgaaaaac tggcaggcat cggctttcat tggggatttt
    48601 ctgaccagag tcatttttca acggtattta agcaacgctt tgggatgacg ccaggcgagt
    48661 atcgacgtaa attccgctaa ctaattggcg ttgtgtgtac tgcaacgcca attagttaaa
    48721 acagatattt gactatgcat ttttcagggc gaaaggcaat ttgacaggag tttttgaata
    48781 ttcaccttgt ttcaggaaaa tagatcaatc gctttctcct gtagcgttat cccgatattg
    48841 cggttttgct attaaaattt tcgcacccat ttaccgttta tcccttgtac atattccccc
    48901 ggtttggccc gagctacgag cttttgcccg gcgagtttcg cgatatcatc tacagacaca
    48961 ttattttgct ttgccagttg ttggtaactc gctttacgtt cggcattaat gtcggttact
    49021 aatttctctg tttcagcatc cgttttcagc gcaaccagat aaccataaaa tgtttcacct
    49081 acccgcccct gagttctggc ttcatccaga gtcaatgcca ttacattgct gcttaccagc
    49141 ccaacaagaa acgcacaaag aagtaatgtt ttcttcatca tcgcctcaga aaagatcgct
    49201 acgagtttca agcagctctt cgacatcttt gtctgccttg atgatgatct catgctcaat
    49261 tttaacgttc atattgatag tgatcggttc cttgggcgca gcgacttcaa tacgcggcgt
    49321 acaggcagcc agcataaaag atgacgtcaa cgcagccagt aaaattttca ttgtttttcc
    49381 tcacactctt ttccttgcgg acagtcattt cccggcagac gtgcgttctg ctccaaccat
    49441 gcctggagat tatcgccaaa gcgtaaactg cgccacagcg taaaaatatt ctcttcatga
    49501 tagtaattaa ggtttaccgt accgctttta ccatcaacgc gactggtacc tgtaatgttg
    49561 gcctgcatgg ttaataaacc gagattatct aagttaattt ttgtcgatga ccggctaatt
    49621 tccatatagc gcaaccagtt aattgctgaa cccgcagtca tattgtcttt caccaccgca
    49681 tctgcggtgt ctttatccag ccgcaaggtc attggcccgc tatttgccag ccaaccatct
    49741 ttcactatcc atttttcgtt gtttagccat aacggcaatg caccactaaa tgccccggac
    49801 atggcgaatt gtttcggatt gacggcgcta accagttcgc tggatgagag attattcagc
    49861 cgcaacagcg ccgggtcatg ttgcggcata cgtaattgtt tcatcagtac gttaccgcct
    49921 aacacatcga cgctaacatc agtcaacagc aagggttctt cttcagtcca cggataacgc
    49981 ccttgcaaat cagccgtaat atttttcgct gtcaccagat taatcacttc ggcaattcgc
    50041 aacgtaacgg ggccgcgagt ccccagatgc caggctccat cggcaaaacg gaaaggcagg
    50101 acaaaatcga caccgttaac ctgattatct ggcatccagg cactaccgcc tttcaacacg
    50161 ccgtgtcctc ccgcgcggaa tccttgttca ggcgcagcag aaaatgcaac ctgagcatac
    50221 agttcaccat cgcgtaagtt catcttccag tcgggtggca ccagcggctg gaatacagtc
    50281 agtgattgtt ttggccacca ggcgttaccg cgcagacgaa taccgtccca gcgaccattt
    50341 acccgaaccg ggccaatctc accagcatgt aaatcgcctt taaagaggaa atacgtcgga
    50401 tcgcgcccat cgacgctaaa ttttaaggtt gatggcggta acacactgcc gccagtgaac
    50461 agcgtttgcc cggcgtccag tgacagcgcg ccgctgaagg agggatgctg tgcgtcacgt
    50521 acccagacga cgggcttgtc gagaattaat cgcggctttt ctaccgtcat cgtaccgtat
    50581 tgtaactggt cgaaaccggt ggagaggtcg gtcagcgtaa tcgtgctgtc atgccattca
    50641 ccttttcctg cgacatccca ggtggcattc atcggtgtaa aactgccttt tccccagtag
    50701 cgccactgcc agcggcccgc gtcagggaga aaatcattcg ccagcccatc catatgcagc
    50761 acgaaatcgc ccagttcatt ttcatgcgcc tgcaaaatgg cctgcaaacg tccgtcaaca
    50821 ccacgttggg tgacttttac acccgctaaa ggccagcgga tttcatcgat gtccagcgaa
    50881 tcgatgactc ttccctttga acgaagtaac gcgcctggct caaaggtcag cgttgggtca
    50941 gacagacttc cacttagctg cgcaggtaaa cgggcatata aaatgaggtc cgcctgtttc
    51001 gcttcaccgg tcagctgcag aggcagctga ctgttatcca tgcttaattt tcctgggcca
    51061 aaatttagca ccgcgttgcc tttacccgct tgcccctggg tcagcacact cagtcgtccg
    51121 ctgatcagag cgttctcaag ccctgcctgc cagttgtcga ctttgacacc cagtcggcca
    51181 ctcaaaggaa aacctgcata cggccaactc cagcgaccat cgcttacggt caattgttga
    51241 cgagtaattt gccacggcaa atcgagcaac ggatcgccgt tatcccgtgc cagcacaatc
    51301 aattgcccgc tattttcctg ccagtccagc tctgcatcca ccagtgacgg ttcctgcggc
    51361 aagtttaacg tcgcagtagc atgcccactt acaggaagtc catccggcac gagcggcata
    51421 gtaaattccc ccaccagttt aaccggcggc tgattttcaa acgcgacgac atccagttcg
    51481 ctgactgtaa gttgttgccc tttcagctgg ccttgaaatt taactttttc gccctgataa
    51541 cgcagttgct ggatatcaga ggttaatgcg agagagagtt ttccctgcca ttcctgccag
    51601 ggagaaaaaa tcagtttatc gatattgatc caggtgttag gcagcatgga ctgccactgc
    51661 gcgagggttt ttggagcagc tggcgattgc tccgtctgcg gcaatttcgc cagacaagca
    51721 gaatcaagtt ctaccgtgcc gacgttcaat aaccagcggc tgggatgtga aaggctggcg
    51781 ttggtgatat gcgcaagctg acaatctccc accagataac ggagatcggg aatgattaaa
    51841 cctttacgcg taatgcgtgg gctttcatct aatgcaatac gtgtcccgag cggtagccag
    51901 atgcccgcca gcgtgggaac ccacagcccg agcgtcatca gcagcgtcaa cggcacaaga
    51961 ataatcagta ataacagcgc gagaacggct ttatatttac ccagcatggg tagttaatat
    52021 cctgatttag cgaaaaatta agcattcaat acgggtattg tggcatgttt aaccgttcag
    52081 ttgaaggttg cgcctacact aagcatagtt gttgatgaat ttttcaatat cgccatagct
    52141 ttcaattata tttgaaattt tgtaaaatat ttttagtagc ttaaatgtga ttcaacatca
    52201 ctggagaaag tcttatgaaa ctcgccgttt atagcacaaa acagtacgac aagaagtacc
    52261 tgcaacaggt gaacgagtcc tttggctttg agctggaatt ttttgacttt ctgctgacgg
    52321 aaaaaaccgc taaaactgcc aatggctgcg aagcggtatg tattttcgta aacgatgacg
    52381 gcagccgccc ggtgctggaa gagctgaaaa agcacggcgt taaatatatc gccctgcgct
    52441 gtgccggttt caataacgtc gaccttgacg cggcaaaaga actggggctg aaagtagtcc
    52501 gtgttccagc ctatgatcca gaggccgttg ctgaacacgc catcggtatg atgatgacgc
    52561 tgaaccgccg tattcaccgc gcgtatcagc gtacccgtga cgctaacttc tctctggaag
    52621 gtctgaccgg ctttactatg tatggcaaaa cggcaggcgt tatcggtacc ggtaaaatcg
    52681 gtgtggcgat gctgcgcatt ctgaaaggtt ttggtatgcg tctgctggcg ttcgatccgt
    52741 atccaagtgc agcggcgctg gaactcggtg tggagtatgt cgatctgcca accctgttct
    52801 ctgaatcaga cgttatctct ctgcactgcc cgctgacacc ggaaaactac catctgttga
    52861 acgaagccgc cttcgatcag atgaaaaatg gcgtgatgat cgtcaatacc agtcgcggtg
    52921 cattgattga ttctcaggca gcaattgaag cgctgaaaaa tcagaaaatt ggttcgttgg
    52981 gtatggacgt gtatgagaac gaacgcgatc tattctttga agataaatcc aacgacgtaa
    53041 ttcaggatga cgtattccgt cgcctgtctg cctgccacaa cgtgctattt accgggcacc
    53101 aggcattcct gacagcagaa gctctgacca gtatttctca gactacgctg caaaacttaa
    53161 gcaatctgga aaaaggcgaa acctgcccga acgaactggt ttaatcttgc cgctcccctg
    53221 cattccaggg gagctgattc agataatccc caatgacctt tcatcctcta ttcttaaaat
    53281 agccctgagt cagaaactgt aattgagaac cacaatgaag aaagtagccg cgctcgttgc
    53341 gctaagcctg ctgatggcgg gatgtgtaag taatgacaaa attgctgtaa cgccagaaca
    53401 gttacagcat catcgttttg tgctggaaag cgtaaacggt aagcccgtga ccaacgataa
    53461 aaatccgcca gaaatcagct ttggtgaaaa aatgatgatt tccggcagca tgtgtaaccg
    53521 ctttagcggt gaaggcaaac tgtctaatgg tgaactgaca gccaaagggc tggcaatgac
    53581 ccgtatgatg tgcgctaacc cgcagcttaa tgaactcgat aacaccatta gcgaaatgct
    53641 gaaagaaggt gcacaagtgg atctgaccgc gaaccagtta acgctggcga ccgcgaaaca
    53701 gacattaact tataagctgg cggatttaat gaattaatag ctgccacagc tcccggcggc
    53761 aagtgactgt tcactacagc gtttgccgtt gggtaatgca cacatcccaa tcgccgtacc
    53821 atccagttga cgggcaacag aaagcgaacc gccgatcatt gcacaatttg cttctccact
    53881 actggacatc gacgctttta aacctggcgc tacgtgcgcc gcagtcgcct gctgaacagg
    53941 ttcactacta cacgccgaca acaataaagc ggcacaccct acccaaaacg ctgctcgcat
    54001 cttttcttcc tctgatcttc aagccaaacg acaccgccat aaataatagg cagcacagag
    54061 ggcgacgtcg agagctgtcc tgcgcgttgc cccgcatttt tactttttta tggctatttt
    54121 tttgccctct gtttgatcaa aacattcatt acgctgatgt gggggacaca aaagcgaaaa
    54181 tgcagaagaa agccatttgc taaaattgaa ggattactac tgggcgcgca gcaatttcgt
    54241 gcgcccctca tttgcgcaat gtaagggtgt catatgatta ctattgacgg taatggcgcg
    54301 gttgcttcgg tggcgtttcg caccagtgaa gttatcgcca tctaccctat tacccccagt
    54361 tccacgatgg cagaacaggc tgatgcctgg gccggaaacg gcttaaagaa cgtttgggga
    54421 gacacaccac gcgtagttga aatgcagtcg gaagcgggtg ctatcgctac cgtgcatggc
    54481 gctttgcaga cgggtgccct ttcaacatcg tttacgtcat cgcagggttt gctgctgatg
    54541 atcccgacgc tgtacaaact ggcaggcgaa ctgacgccat ttgtcctgca tgtagcggca
    54601 cgtaccgttg ccacacatgc actttctatt tttggcgatc attccgacgt tatggcggtg
    54661 cgccagacgg gttgcgcgat gttgtgtgcg gctaacgtcc aggaagcgca agattttgct
    54721 ctcatttcgc acatcgcaac gctgaagagc cgcgtgccat ttattcattt ctttgatggt
    54781 ttccgcacgt cccacgaaat caataaaatt gtcccgctgg ccgatgacac tattcttgat
    54841 ctcatgccgc aggccgaaat tgatgctcat cgcgcccggg cgctcaaccc ggaacatccg
    54901 gtgatccgcg gtacgtccgc caatcctgac acttatttcc agtctcgcga agccaccaac
    54961 ccatggtacg acgcggtcta tgaccatgtt gaacaggcga tgaatgattt ctctgccgcg
    55021 acaggtcgtc agtatcagcc gtttgaatat tacgggcatc cgcaagcgga acgggtgatt
    55081 atcctgatgg gctctgccat tggcacctgt gaagaagtgg ttgatgaatt gctaacccgt
    55141 ggcgaaaaag ttggcgtgct gaaagttcgc ctgtaccgtc ccttctccgc caaacacttg
    55201 ctgcaagctc tgccgggatc cgtacgcagc gtggcggtac tggacagaac caaagaaccc
    55261 ggtgcccagg cagaaccgct ctatctggat gtaatgaccg cactggcaga agcctttaat
    55321 aatggcgagc gcgaaactct gccccgtgtc attggtgggc gctatggtct ttcatccaaa
    55381 gaatttggcc cggactgtgt actggcggta tttgccgagc tcaacgcggc taaaccgaaa
    55441 gcgcgcttta cggttggtat ttacgatgat gtgaccaatc tgtcactgcc gttgccggaa
    55501 aacaccctgc caaactcggc gaaactggaa gccttgtttt atggccttgg tagtgatggc
    55561 agcgtttccg cgaccaaaaa caatatcaag attatcggta attccacgcc gtggtacgca
    55621 cagggctatt ttgtttacga ctccaaaaag gcgggcggcc tgacggtttc tcaccttcgc
    55681 gtgagcgaac agccgattcg ttccgcttat ctcatttccc aggctgattt tgttggctgc
    55741 caccagttgc agtttatcga taaatatcag atggctgagc gtttaaaacc tggcggcatt
    55801 ttcctgctca acacgccgta cagcgcagat gaagtgtggt cgcgcttgcc gcaggaagtt
    55861 caggccgtgc taaatcagaa aaaagcgcgc ttctatgtga ttaacgcggc gaaaatcgcc
    55921 cgcgaatgtg gcctggcggc ccgtattaat accgtcatgc agatggcttt tttccatctg
    55981 acgcaaattc tgcctggcga tagcgccctc gcagaattgc agggtgcgat tgccaaaagt
    56041 tacagtagca aaggccagga tctggtggaa cgcaactggc aggctctggc gctggcgcgt
    56101 gaatccgtag aagaagttcc gttgcaaccg gtaaatccgc acagcgccaa tcgaccgcca
    56161 gtggtttccg atgccgcccc tgatttcgtg aaaaccgtaa ccgcagcgat gctcgcaggg
    56221 cttggtgatg ccctccccgt ttcggcgctg ccgccagacg gcacctggcc gatgggcacc
    56281 actcgctggg aaaaacgcaa tatcgccgaa gagatcccca tctggaaaga ggaactctgt
    56341 acccaatgta accactgcgt cgccgcttgc ccacactcag ctattcgcgc aaaagtggta
    56401 ccgcctgaag cgatggaaaa cgcccctgcc agcctgcatt cgctggatgt gaaatcgcgt
    56461 gatatgcgcg ggcagaaata tgtcttgcag gtggctccgg aagattgcac cggttgtaac
    56521 ctgtgcgtcg aagtttgccc ggcgaaagac cgtcagaatc cagagattaa agccatcaat
    56581 atgatgtcgc gcctggaaca tgtcgaagaa gagaaaatca attacgattt cttcctcaac
    56641 ctgccagaaa tcgaccgtag caaactggaa cgtattgata ttcgtacatc gcagctgatt
    56701 acaccgctgt ttgaatattc aggtgcttgc tccggttgtg gcgagacgcc gtatattaaa
    56761 ttactgactc agctctatgg cgaccggatg ttgatcgcta acgccaccgg ctgttcttca
    56821 atttatggcg gtaacctgcc ctctacaccg tataccaccg atgccaacgg tcgtgggccg
    56881 gcatgggcga actctctatt tgaagataat gccgaatttg gccttggttt ccgcctgacg
    56941 gtcgatcaac accgtgtccg cgtgctgcgt ctgctggatc aatttgccga taaaatcccg
    57001 gcggaattac tgacagcgtt gaaatcagac gctacgccag aggttcgtcg agaacaggtc
    57061 gcagctttac gccagcaact caacgatgtt gccgaagcac atgaactgct gcgtgacgca
    57121 gatgcactgg tggaaaaatc aatctggctg attggtggcg acggctgggc ttacgatatc
    57181 ggctttggcg gtctggatca tgtattgagt ttgacggaaa acgtcaacat tctggtgctg
    57241 gatacgcaat gctattccaa caccggtggt caggcgtcga aagcgacacc gctgggtgca
    57301 gtaactaagt tcggcgagca cggcaaacgt aaagcgcgta aagatcttgg cgtcagtatg
    57361 atgatgtacg gtcatgttta tgtggcgcag atttctctcg gcgcgcagct gaaccagacg
    57421 gtgaaagcga ttcaggaagc ggaagcgtat ccggggccat cgctgatcat tgcttatagc
    57481 ccgtgtgaag agcatggtta cgatctggca ctcagccatg accagatgcg ccaactcaca
    57541 gctaccggct tctggccgct atatcgcttc gatcctcgtc gtgccgatga aggcaaactg
    57601 ccgctggcgc tggattcacg cccgccgtca gaagcactgg aagaaacgtt acttcacgag
    57661 caacgtttcc gtcgtctgaa ttcgcagcag ccagaagtgg cagaacagtt atggaaagat
    57721 gctgcagctg atttgcaaaa acgctatgac ttcctggtac aaatggccgg aaaagcggaa
    57781 aaaagcaaca ccgattaaat gctctggata aggattatcc aattctaaaa aaaaagcccg
    57841 gacgactgtt cgggcttgtc tttttatatg ttggaaaatc agtggcaatg caatggccca
    57901 acagaaaata aaatctgtga caataaaggc atataacccg cgcagaataa cgtatagcaa
    57961 attaaaagta taatttttac tttgtatgta ttatcttcga tacattcacc aaacttatta
    58021 tcatttcaga ggaattattt gattaaggtt ttacttaagg cgtaacaaat gatatttatc
    58081 ggctaactga acttctcctt taggatgttt tcactccccg aaatggggat aaaaggcaaa
    58141 taaaataact aaaggattta ttcaatgaaa agcaaagtac tggcactttt aattcctgcc
    58201 ctgctcgccg caggtgctgc acatgcagcc gaagtttata ataaagacgg caacaaatta
    58261 gatctgtatg gcaaagttga tggcctgcat tatttttctg ataattcggc gaaagatggc
    58321 gaccagagct atgcgcgtct gggttttaaa ggcgaaaccc aaattaacga tcaactcact
    58381 ggctacggtc aatgggaata caatattcag gcaaacaaca ctgaatcttc aaaaaaccag
    58441 tcatggaccc gtctggcatt tgccgggctg aaatttgcag attacggttc tttcgattac
    58501 ggacgtaatt atggcgtaat gtacgacatc gaaggctgga ccgatatgct gcctgaattt
    58561 ggcggtgact cttataccaa tgcagacaac tttatgactg gtcgagccaa tggcgtcgcg
    58621 acttatcgta atactgattt cttcggtctg ataaatggtc tgaacttcgc ggtgcagtat
    58681 caaggtaaca acgaaggtgc cagtaatggt caggaaggca ccaacaacgg acgtgatgtt
    58741 cgccatgaaa acggtgacgg ctggggtctt tccacaacat atgatttagg catgggcttt
    58801 agcgctggtg cggcatacac ctcttctgac cgcaccaatg atcaggttaa ccatactgcg
    58861 gcgggtggtg ataaagcaga cgcgtggact gctgggctaa aatacgatgc taacaatatt
    58921 tacctggcaa ccatgtattc agaaacgcgt aatatgaccc cgtttggcga cagcgattat
    58981 gctgttgcaa acaaaaccca gaactttgaa gtcactgcac agtaccagtt tgattttggt
    59041 ctgcgtccgg cagtctcttt cctgatgtct aaaggccgtg acctgcacgc tgccggtggt
    59101 gcagacaacc cggcaggtgt tgatgataaa gatctggtta aatacgccga tgttggcgcg
    59161 acttactatt tcaataaaaa catgtccact tatgttgact ataaaatcaa cctgttggat
    59221 gaagatgaca gtttctacgc tgccaatggc atctctaccg atgatattgt cgctttaggt
    59281 ctggtttatc agttctaaat cctcctgccc gctgttatgg cgggcttttt ctgcttattc
    59341 ttcctccttt gatctaaatt aaaaatgtga actccgtcat tacacaaaaa gtgtcatctg
    59401 gcgttacact ttatgcggat actaaaacag gaggttttat gaacagaacg attcttgtcc
    59461 ctatcgatat ttccgattca gaattaactc aacgcgtgat tagccacgtt gaggcagagg
    59521 caaagattga tgatgcagag gttcatttcc tgacggtaat accttcactg ccctactatg
    59581 cctctctggg tttagcgtat tccgcagaat taccggcaat ggatgacctg aaagcggaag
    59641 ccaaatcgca actggaagag atcattaaaa aatttaaact gccaaccgac agagtgcatg
    59701 tccatgttga ggaaggctcg cccaaagacc gcattctgga aatggcgaag aagatccccg
    59761 ctcatatgat catcattgct tcccatcgac cggatatcac cacttatctg ctcggttcca
    59821 acgccgcagc tgtagtgcgt cacgcagagt gctccgtgct ggttgtgcgc tgacactaac
    59881 gcccgcacat tgctgcgggc tttttgattc atttcgcaaa tgtgctgaca ttttcccctc
    59941 taatccgtac catacacgcc actgttttta tatcagattt cttatgctgg gcattccggc
    60001 atgacgcata ctcttctgat gccatataac gaattgagtc gcttttaaat gtcgcaaaat
    60061 caagaaatta gtaagaaaga acaatacaac ctgaacaaat tacaaaaacg tctgcgtcgt
    60121 aacgtgggcg aagccattgc tgacttcaat atgattgaag aaggcgatcg catcatggtt
    60181 tgcctctccg ggggtaaaga cagctatacc atgctggaga ttctgcgcaa tttgcagcaa
    60241 agcgcgccaa tcaatttttc gctggtggct gttaacctcg atcaaaagca accgggcttc
    60301 ccggaacacg ttctgcccga gtatcttgaa aagctgggcg ttgagtacaa gattgttgaa
    60361 gagaatactt acggtatcgt gaaagagaag attccagagg gcaaaaccac ttgctcactg
    60421 tgttctcgcc ttcgtcgcgg tatcctttat cgtaccgcaa cggaactggg ggcgacgaag
    60481 atcgcgttgg gtcaccatcg tgacgatatc ctgcaaacgt tgttcttaaa tatgttctac
    60541 ggcggcaaga tgaaaggtat gcctccgaaa ctgatgagcg atgatggcaa acatatcgtt
    60601 attcgtccgc tggcctactg ccgcgagaaa gatattcagc gatttgccga tgcaaaagcg
    60661 ttcccgatta ttccgtgcaa cctgtgcggt tcacagccta acctgcaacg tcaggtgatt
    60721 gctgacatgt tgcgtgactg ggataaacgt tatccagggc gtatcgagac gatgttcagc
    60781 gcgatgcaga atgtggtgcc gtcgcatctg tgcgatacca acctgttcga tttcaaaggc
    60841 attacccacg gttctgaagt ggttaacggg ggtgatctgg cgtttgatcg cgaagagatc
    60901 ccactacaac cggcgggctg gcagccagaa gaagatgaaa atcagttgga tgagttacgg
    60961 ctgaatgtgg ttgaagtgaa ataaccagga tagcgcccga tgcgcaagcg tatcgggcta
    61021 ctcttatgga ggccggataa gacgcggcca gcgtcgcatc cggcaatccc gcataagatg
    61081 tttactcttg cacccggcaa ttcaacattt cattatttta ataaccgcac ccggcacgtt
    61141 tttcctttaa tcttcccgcc cgtaactgtt tccatgcttt atgagcaaca gcctgacgga
    61201 ccgcgacata gacatgcgcc ggatgcacgg cgattttgcc aatatctacg ccatcaagcc
    61261 cgatgtctcc tgtcagcgca cctaatacat cacccgggcg cattttggct tttttcccgc
    61321 catcgataca caacgttgcc atttctgctt ccagcggcaa aatggaacta ttagctggcg
    61381 gcgtttgcca gttaagtttt atctgcaaca tgtcagaaat gatattggcc cgctgtgctt
    61441 cttccggagc acagaaactg atcgccagac cgctatttcc tgcacgagct gtacgaccga
    61501 tgcgatgtac atgaacttca gggtcccacg ccagctcaaa gttcaccacc agctcaagcg
    61561 atttaatatc cagaccacgc gcagcaacat cagtcgcaac cagtacacgg gcgctaccgt
    61621 tagcaaaacg taccagggtc tgatcgcgat cgcgttgctc cagatcgccg tgtaacgaca
    61681 atgcactttg ccctacttca ttcagcgcgt cgcagacagc ctggcaatct tttttggtat
    61741 tgcaaaacac cacacaagag gatggctgat gcaaacttaa taaccgttgc aacagaggaa
    61801 ttttgccttt actggatgtc tcataaaatt gttgttcaat gggtggcaaa gcatctgttg
    61861 agtcaatttc aatcgccaaa ggatcgcgtt gcactcgtcc gctgattgcg gcgatggctt
    61921 ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa cggatgacat
    61981 catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc atcaccaaag
    62041 tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa cgacccggcg
    62101 ttgccacgat aatatgcggc gcatgttgca acgaatcacg ctgcataccg aacggttgac
    62161 caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga cgcaattcac
    62221 ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg gtttgaaata
    62281 gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg ccgctgccgg
    62341 ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg gcggcctgca
    62401 ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg ggaggcaaaa
    62461 cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc aaagttagca
    62521 ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt cttcatcagg
    62581 tggtgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa caggatcgcc
    62641 aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat ttccctccgg
    62701 tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa aaagccgact
    62761 catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa aaggaagtaa
    62821 gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag ggagatattg
    62881 ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt ttgtatacag
    62941 acagccggaa aaattgcttt tgttacaacc atttactacg gtgcaaccat aaagcaacac
    63001 caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc cacccgccgc
    63061 cagggatccc accaaggttg acgccaaata accctgtcag aaaggtactg ggtaaaaaga
    63121 ccattgccat caatgacatt gtataggtac gacgagctaa attttcctgc atcacctgag
    63181 cgatttcatc cgccatcacg ccagtccgtg ctatacaggc gtcgatttcg tcaagaccgc
    63241 gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc atccacggca
    63301 aacgttcact ggcaagacga gcataaacat cacgttgcgg tgccatatag cgacgcatca
    63361 cgattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc tgatcaagga
    63421 gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattcg ctggaatgat
    63481 cggtcaacgc atcgcacaca tccaccagcc accctccgca atcggtcgga cccgtgccct
    63541 cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg gtcgaaacaa
    63601 ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt tcatcggtgc
    63661 tgccgtttat acagcgcaat gtaataagcg tgccttcacc gagacggctg actcggggcc
    63721 gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt gttgtcgcca
    63781 gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga tgcgcttcat
    63841 caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc atccaggcaa
    63901 atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccacagtg cctccatcat
    63961 caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc atcaccccat
    64021 taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa aatgcttttc
    64081 cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt atctgatcat
    64141 atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa cgggatctca
    64201 atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg gattgtttcg
    64261 ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat gcaagacctc
    64321 acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga actgccgaaa
    64381 tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt tcagcgtaaa
    64441 gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga aacagaaccg
    64501 gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc cagtcgctcg
    64561 gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac taactctgca
    64621 ccaatgttgt tgattgaccc ctcacgagat ggccaaatcg tcgatgctaa cctcgccgcg
    64681 ctcaatttct atggttataa ccatgaaacg atgtgccaga aacatacctg ggaaattaat
    64741 atgctagggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc cggcggtcat
    64801 aaacctttga attttgttca taaactggcg gatggatcta ctcgtcatgt gcagacctat
    64861 gccgggccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca tgatattact
    64921 gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc gatgaccgga
    64981 ttactgaatc ggcgacagtt ttatcacatt accgagccag gccaaatgca gcatctcgcc
    65041 atcgctcagg attacagctt gttgctcatc gacaccgatc gttttaaaca cattaacgat
    65101 ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgcac ccttgaaagt
    65161 tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt cttattgcta
    65221 ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgtgt aagcgtggca
    65281 aaagtgagta tttcgggctt accacgcttt accgtcagca ttggtgtggc gcatcacgaa
    65341 ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta tcgggcgaaa
    65401 aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg agatcaacga
    65461 ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt acgtagtgcg
    65521 acgtaacacg ccccgctgcc gccatgatgc ggtagcgcag tgcaatatgc ctgaacatca
    65581 tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg cgatttatca
    65641 tcccgtcctt tgccatgaat aatcagcacg ttacgcaaac catcagccat ggcttgttga
    65701 atgaaactga acatcatttt gcggcactct tccaccggct ggcgcaaaag attcaggctc
    65761 gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgttg caacccttcc
    65821 cgccgaaact ccagcggctg acttagtggg atgatgtcga gaaatccggt ggtgaggaaa
    65881 ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt tggatgccag
    65941 tggacatcgg tagcacgttt cagcggctgg acatcttcca tggcgtcaag aaacagcgat
    66001 ttgtcgtcaa ggttcatgtt acatcctccc gcaattaaga gcgcgatatg ataaccagac
    66061 cgggtcggtc caacaacgta ttacccaaaa ttccagtaat aagttccaaa tattgccgat
    66121 attttaagca aaatgcttat gcatgattat tcattcacga tattaataat gtaacttata
    66181 ttttcgtgaa atctgtcact gaagaaaatt ggcaactaaa ggttaaaacc gttataacac
    66241 agtcaccggc gcagaggaga caatgccgga cttaagacgc ggatgcactg ctgtgtgtac
    66301 tgtagagtct ggcggatgtc gacagactct atttttttat gcagttttaa ctttgcagat
    66361 agccgcattc tcgccggaat tgcgtgatta atgacgcggt cagtggtgtc tggcgactat
    66421 cacgccgctg aatcaaataa taggccgctt tcggtaaaat ttcgctaacc ggcaacatca
    66481 ccagcccctg tccgtgcaag ggatcgcagc ccatttcttc aggcagtatg ctgagaaaat
    66541 cgctttttgc caccagactg atacaggctg agaacgtctc gcagactaca ccgacctgtg
    66601 gcgtttgcgc ctgatcgtca agcaattcac tcaactgttt gtagtaactg ccgtgtggcg
    66661 tcggcattgt ccagctgtaa tccagtaact gtttgatcga acgggcacca atggcggggt
    66721 gtcccgggcg gcaaaagatc gcgaattgct tttccagtaa tttctcaaaa gtaaattcgt
    66781 ggtcgtacgg tccctgataa taggtattga tggtgaaatc caattctccc tgacgcaatt
    66841 cattaatcat cgacaccagt tgcccttcca taatgcgtac ttttacctgc ggatgctgct
    66901 gatgaaaacg agatatgaca gctggcatca gactgcggga aatactggcc cccatgccga
    66961 tattaatctg ccctgccagt tgcccttgtc gttggcgaat ttcctcttgc gctgcgcgca
    67021 gctcttcaag aataagactg gcgtgctgat aaaaactttc accggcatca gttaacgtca
    67081 cgcctttact acggcgaaaa aagagttgcg ccgctaatcc ttcttccagc tcctgaatag
    67141 atttactcag tgccggttgc gacatattca acattcggct cgctccgcga atgctgccct
    67201 gacgagccac ttcaacaaaa gcacgaattt gatgaatttt tacctgaaaa gccatgacgc
    67261 caccgataac cgttatttat cagaccaaag aaactggcat ctactttaat gcagatgatt
    67321 gtgtcagggt aatttatgaa cggttaaaac tgtgaaaaat cagttagtga taagtaaaaa
    67381 ctatcgctac gtgaaccggg tcacactttt tactgatgac gggaaaggtt atggagtctt
    67441 tgaatcaatt tgttaattcg cttgccccaa aattatcgca ctggcgacgt gattttcatc
    67501 actatgcaga gtctggctgg gtggaattcc gcactgccac ccttgttgcg gaagaattgc
    67561 agcagctcgg ctattcactg gcgctgggcc gcgaagtagt taatgaaagt agccggatgg
    67621 gactacctga tgaactcact ctacaacgcg aattcgagcg cgctcgtcaa cagggtgcgc
    67681 tagcacaatg gattgcggtt tttgaaggtg gtttcactgg tatcgtcgcc accctggata
    67741 ccggtcgccc cggtccggtg atggctttcc gtgtcgatat ggacgcgctg gatctcagtg
    67801 aagagcagga tgtcagccat cgcccctacc gcgacggttt tgcgtcatgt aacgccggaa
    67861 tgatgcatgc ctgtggtcat gatggacata ccgccattgg gcttgggctg gcgcataccc
    67921 ttaaacaatt cgagtccgga ctacatggcg tcatcaaact gatttttcag cctgcagagg
    67981 aaggtacgcg tggcgcgcgg gcgatggtcg atgcaggtgt cgtagatgat gttgattatt
    68041 ttactgccgt gcacattggc actggcgtac ctgcgggcac cgtggtgtgc ggcagtgata
    68101 attttatggc aaccaccaaa tttgacgcgc acttcaccgg gaccgccgct cacgcaggcg
    68161 caaaaccaga agacggtcac aatgccttgt tggcggcagc acaagcaact cttgcactgc
    68221 atgcaatcgc cccgcacagc gaaggagctt ccagagtaaa cgtgggcgtt atgcaggcag
    68281 gaagcggccg taacgttgtt cctgcctcgg cgttgctgaa agtggaaaca cgcggggcca
    68341 gcgacgtcat taatcaatat gtttttgacc gtgcacaaca agcgattcag ggcgcagcaa
    68401 ccatgtatgg tgtcggcgtt gaaactcgtc tgatgggtgc agctaccgcc agttctcctt
    68461 cgccgcaatg ggtcgcatgg ttgcaaagtc aggcggctca ggtcgcgggg gtcaatcagg
    68521 ccattgaacg tgttgaagcg cctgcaggtt ccgaagatgc cacattaatg atggcccgcg
    68581 tgcagcaaca tcaagggcaa gcctcctacg tggtgtttgg cacacagctg gcggcaggtc
    68641 atcacaacga aaaattcgat tttgacgagc aggttctcgc tattgccgtc gaaacgctgg
    68701 cgcgcaccgc gctcaatttt ccctggacgc gaggtatctg atgcaggaaa tctatcgttt
    68761 tatcgacgat gcgattgaag ccgatcgcca acgttatacc gatattgccg atcaaatctg
    68821 ggatcatcca gaaacacgtt ttgaagagtt ctggtcagcg gagcatctgg cttcggcgct
    68881 ggaatctgca agcttcaccg ttacccgcaa cgtaggcaat atcccaaatg catttattgc
    68941 ttcgtttggt caaggcaaac cggttatcgc cctgctggga gaatatgacg ccctggcagg
    69001 tttaagtcag caagctggtt gcgcgcaacc tacatccgcg acgcccggtg aaaatggtca
    69061 cggttgcgga cacaatttgc tgggaaccgc cgcctttgcc tctgcaatag ccgtcaagaa
    69121 atggctggaa caatatgggc aaggcggcac agtgcgcttt tatggttgtc ctggcgaaga
    69181 aggcggctcg ggtaaaacgt tcatggtccg cgagggggta tttgatgatg tggatgcggc
    69241 actcacctgg cacccggaag cctttgccgg catgttcaat acccgcacgc tggcaaacat
    69301 tcaggcatca tggtgcttta aagggatcgc agcacatgcc gcgaattccc ctcatttggg
    69361 acgcagcgcc cttgatgccg taacgttgat gaccactggc accaacttcc tcaacgaaca
    69421 tattattgaa aaagcgcgcg tacactatgc catcacaaat agcggcggga tctcgcccaa
    69481 cgtggtccag gcgcaggcag aagtgcttta tcttatccgt gcccccgaaa tgaccgacgt
    69541 gcagcatatt tatgatcggg tcgccaaaat cgccgaaggt gcggcattga tgaccgaaac
    69601 cacggttgaa tgccgcttcg acaaagcctg ttccagttat ctcccgaatc gcaccttaga
    69661 aaatgccatg taccatgccc tatcccattt tggtaccccg gaatggaact ccgaagaact
    69721 ggcttttgcg aaacaaattc aggctacgct cacccccaac gatcggcaaa acagtctgaa
    69781 taatatcgcc gcaaccggtg gcgaaaacgg caaggttttt gcactacgtc atcgtgaaac
    69841 ggtactggcg aatgaggtcg ctccatatgc cgccaccgat aacgtgcttg cggcatcgac
    69901 tgatgtcggc gacgtcagtt ggaaactgcc tgttgcccag tgtttcagcc cctgttttgc
    69961 cgtcggtaca ccgctacata cgtggcaact ggttagccag gggcgaacat ctattgctca
    70021 taaaggaatg ctgctggcgg cgaaaactat ggcagcaacc acagtcaatc tcttcattga
    70081 ttcagggcta ttgcaagaat gccaacaaga gcatcagcaa gttacggaca cgcaaccgta
    70141 tcactgccct atcccgaaaa acgtgacacc gtcaccttta aaataacaac aacgcaaaca
    70201 caacaaccga ggaatgccca tgagtatgtc atccataccg tcgtcctccc aatccgggaa
    70261 gctctatggc tgggtcgaaa gaattggtaa caaggttccc catccttttt tgctctttat
    70321 ctatttgata atcgtactca tggtgacgac ggcaattttg tcagcctttg gcgtcagtgc
    70381 gaaaaacccg accgatggta cgccggtggt ggtgaaaaac ctgctcagtg tggaaggatt
    70441 acactggttt ttacccaatg tgattaaaaa ctttagcggt tttgctccac ttggagcgat
    70501 cctggcgctg gttttaggtg ccggcctagc ggagcgcgtc ggcttactgc cagcgctaat
    70561 ggttaaaatg gcatcgcatg ttaatgcccg ctacgccagt tatatggtgc tgtttattgc
    70621 ttttttcagc cacatttctt ccgatgcggc gttagtgatc atgccaccga tgggtgcgct
    70681 gatttttctg gcggtgggca ggcatccagt tgcaggttta ctggctgcca ttgcaggcgt
    70741 aggttgcggc tttacggcta atttactgat tgtcacaacc gacgtgttgc tgtcggggat
    70801 cagcacggaa gcggcggctg cgttcaatcc gcaaatgcac gtcagtgtaa ttgataactg
    70861 gtattttatg gccagctccg tagtcgtact gacgattgtt ggcggcttga taaccgacaa
    70921 aatcatcgag ccacggttag gtcaatggca gggaaacagc gatgagaaac tgcagacatt
    70981 gaccgaaagt cagcgttttg gtttacgcat agcaggtgtc gtatcgctac tttttattgc
    71041 tgcgatcgcg ctgatggtga tcccggaaaa cgggatattg cgcgatccga ttaatcacac
    71101 cgtgatgcca tcacccttta ttaaaggtat cgtgccacta atcattcttt ttttctttgt
    71161 ggtttcgctg gcttatggca tcgctacccg cacaattcga cgtcaggcgg atttaccgca
    71221 tttaatgatt gaaccgatga aagagatggc gggatttatc gtgatggttt ttcccctcgc
    71281 ccagtttgtc gccatgttta actggagcaa catggggaaa ttcatcgccg tggggctgac
    71341 cgatgtactt gaaagttcag ggcttagcgg catcccggcg tttgtcggtc tggcgttgct
    71401 ttcctctttc ttatgcatgt ttattgccag cggttccgca atctggtcga ttctggcccc
    71461 cattttcgta ccaatgttta tgctacttgg ctttcacccg gcatttgcgc aaatcctctt
    71521 tcgtattgcc gactcatccg tattgccttt agcgccggta tctccttttg ttccactgtt
    71581 tcttggattc ctgcaacgct acaaaccaga cgcgaaactg ggtacttact attcgttagt
    71641 cttgccctat ccgcttatct ttttggtggt atggctgctg atgttgctgg cgtggtatct
    71701 tgttggtctg ccgataggtc cggggattta cccacgtttg tcttaagaga gaacggatgc
    71761 tgagattact tgaagaaaaa attgccacgc cactgggtcc actgtgggtg atttgcgatg
    71821 agcaatttcg cctgcgggcg gttgaatggg aagagtacag cgaacgcatg gtgcagctgc
    71881 tggacatcca ttatcgcaaa gaaggctatg agcgcatttc tgccaccaac ccaggcggtt
    71941 taagcgacaa gcttcgtgat tattttgccg gtaatcttag cattattgat acgcttccca
    72001 ctgccacagg ggggacgcca tttcagcgcg aagtctggaa aactctacgc actatcccct
    72061 gcgggcaggt aatgcattac ggccaactgg ctgaacaatt gggccgccct ggcgcggcgc
    72121 gtgccgttgg tgcggcaaac ggatcgaatc ccatcagcat cgtcgtacct tgccatcggg
    72181 ttattggccg aaacggcacc atgaccggat atgcaggcgg agttcagcga aaagagtggt
    72241 tattgcgcca cgaaggttat cttttgctgt aaacattaaa caatttgtgc cagcttgttc
    72301 acacttttat gtaaagttac ccttaacaac ttaagggttt tcaaatagat agacatatat
    72361 ttacatctaa tatcggaatt ctctgctgtt aaggtttgct tagacttact tgctccctaa
    72421 aaagatgtta aaattgacaa atatcaatta cggcttgagc agacctatga tcccggaaaa
    72481 gcgaattata cggcgcattc agtctggcgg ttgtgcgatc cattgccagg attgcagcat
    72541 cagccagctt tgcatcccgt tcacactcaa cgaacatgag cttgatcagc ttgataatat
    72601 cattgagcgg aagaagccta ttcagaaagg ccagacgctg tttaaggctg gtgatgaact
    72661 taaatcgctt tatgccatcc gctccggtac gattaaaagt tataccatca ctgagcaagg
    72721 cgacgagcaa atcactggtt tccatttagc aggcgacctg gtgggatttg acgccatcgg
    72781 cagcggccat cacccgagct tcgcgcaggc gctggaaacc tcgatggtat gtgaaatccc
    72841 gttcgaaacg ctggacgatt tgtctggtaa aatgccgaat ctgcgtcagc agatgatgcg
    72901 tctgatgagc ggtgaaatca aaggcgatca ggacatgatc ctgctgttgt cgaagaaaaa
    72961 tgccgaggaa cgtctggctg cattcatcta caacctgtcc cgtcgttttg cccaacgcgg
    73021 cttctcccct cgtgaattcc gcctgacgat gactcgtggc gatatcggta actatctggg
    73081 cctgacggta gaaaccatca gccgtctgct gggtcgcttc cagaaaagcg gtatgctggc
    73141 agtcaaaggt aaatacatca ccatcgaaaa taacgatgcg ctggcccagc ttgctggtca
    73201 tacgcgtaac gttgcctgat ttttccgcat aactcactat ccttctgtca tatcattaaa
    73261 tttttctgat ttattgatct ggcagaaggt tcatcactgt ttcattcacc agatatgggt
    73321 taatctttta attacaaact gcgttgacag ttgttgtaag gagaccctgt atggctatgt
    73381 atcagaacat gctcgttgtt atcgatccta accaggacga ccaaccagca ttgcggcgag
    73441 ctgtttattt acatcaacgg attggtggca aaattaaagc ctttttgccg atctatgact
    73501 tctcatacga aatgaccacc ctgctctccc cggacgaacg taccgctatg cgtcagggcg
    73561 tcatcagcca gcgtacagcc tggatccacg agcaggcaaa atattatctc aatgctggcg
    73621 ttcccattga aattaaagtg gtctggcata accgtccctt cgaagccatc attcaggaag
    73681 tgatcagcgg cgggcacgat ttggtgctaa aaatggcgca ccaacatgac cgtctggaag
    73741 cggtgatttt tacgccaacg gactggcatc tgttacgcaa atgcccaagc ccggtgtgga
    73801 tggtgaaaga ccagccgtgg ccggaaggag gcaaggcgct ggtggcggtg aatctcgcca
    73861 gtgaagagcc gtatcataat gcgctcaatg aaaaactggt caaagagacg atcgaactgg
    73921 cagaacaagt caaccatacc gaagttcatc tggttggcgc ttatccggta acgccaatta
    73981 atatcgcgat tgaactgccg gaatttgatc cgagcgttta taacgatgcc attcgtgggc
    74041 aacatttgct ggcaatgaaa gctctgcggc agaaattcgg cattaatgaa aacatgacgc
    74101 acgtagaaaa aggtctgcca gaagaggtga ttcctgattt ggcggagcac ttacaggcgg
    74161 ggattgtggt tctgggcacg gtaggacgca ccggtatttc agcagcattc ctcggcaaca
    74221 cggcggaaca ggtgattgat catcttcgct gcgatctgtt ggtcattaaa cctgaccagt
    74281 atcagacacc cgttgaactg gatgacgaag aagacgatta acgactcttc aataactgac
    74341 aaggcccgag ttatcgggcc tattttttac ttactgctct tcgccgccaa ggaaataaat
    74401 tcccaacggg attgccagca acacagtaaa gaccaggcta taaacaaaaa tcatcgatga
    74461 ctgaatcaca tacattgatg tcgtccactc agacagcgga atattgtact gttctataac
    74521 gccgccgata gtcgcgcgag tcacaaccga cgccgcaata atgaaaaccg ccaacaaacc
    74581 aaagagaaat ttcttccctt tcgctgactt cagtttcgcc ataatcatga tgtaatcctt
    74641 gtgcagacaa atttcttgtt acctaaacgt aacaatacca tgacatttca ccgttgtctg
    74701 ctctgaaaac ctcacctctt agctggattt acaatcaacg cattgattag ttagctaatg
    74761 ttgctacttc cttccctgac tattgatgat ctgaatgaat tgtcgcggta aaatagcccc
    74821 gccaggggtt tccggcatga cgacaattat ctggaggagc ctgataaaac aatgatcgct
    74881 gaactgttta caaataatgc gcttaatctg gtcattattt tcggtagctg cgcagcattg
    74941 attctgatga gcttttggtt tcgccgcgga aatcgtaaaa gaaaaggttt tttattccat
    75001 gcggtgcaat ttttaatcta caccataatt atcagtgctg ttggtagcat tattaattat
    75061 gttatagaaa actataaact caaatttatc accccaggcg ttatcgattt tatctgtacg
    75121 tccctgattg cggttattct gacgattaag ctatttttgc tgattaatca gtttgaaaaa
    75181 cagcagatta aaaaaggtcg cgatatcacc agtgcgcgga ttatgtcgcg cattatcaaa
    75241 atcaccatta ttgtggtgct tgttctgctt tatggcgaac atttcggcat gagcctttct
    75301 ggcttgctga cctttggtgg tattggtggt ctggctgtcg gtatggccgg taaagatatt
    75361 ctgagtaatt tcttttccgg gatcatgctc tatttcgacc gtcctttcag tattggcgac
    75421 tggatccgtt caccggacag aaatatcgaa ggtacagtag cggaaattgg ctggcgaatt
    75481 accaaaatta cgacctttga taatcgtcca ttgtacgtac cgaactcgct gttttcgtcg
    75541 atcagcgtag aaaacccagg acgaatgacc aaccgccgca ttaccacgac cattggttta
    75601 cgttatgagg atgcggcaaa agtgggcgtt attgtcgaag ccgtacgtga gatgctgaaa
    75661 aatcacccgg ccatcgacca gcgacaaacc ttactggttt atttcaacca gtttgccgac
    75721 tcgtcattga atattatggt ttattgcttt accaaaacca cggtatgggc tgaatggctt
    75781 gctgcacagc aagacgttta tttgaagatt atcgatattg tgcagtcaca cggcgcggac
    75841 tttgccttcc ccagccagac gctgtatatg gataacatta caccgcagga acaggggcgc
    75901 taaatcagac atcactggcg ttatccacaa gggtaacgcc agttcccatc acatcaatgc
    75961 ttcacaatat acatagtccg gctgtacgcc acatcttccg ggttattaat gggataacct
    76021 ttcagccacg gcttgattaa tcgtccattg gtatattgat aaattggtgc aatcggtgct
    76081 tgctccatga ggattttttc tgccgcgttg taatcggcat tacgcgcttt aacggtattt
    76141 tccgtcgatg cctgggccag aactttgtcg tatgccggat tgttaaagcg tgaaatattt
    76201 cctgaatgcg ttgacgttaa taatgtcagg aaagtggagg gttcattata atcccccacc
    76261 catgaggcgc ggataacatc aaaattgccg gtgttacggc tatcgatata ggttttccat
    76321 tcctgatttt gcaatttaac atctacgcca aggttctttt tccacatcga tgctacagca
    76381 atcgcaattt tttgatggtt ttctgaagtg ttatacaaaa gcgtcagctt cagcggtttt
    76441 tgcggaccat aaccagctgc gctcaacaaa gtttttgcct gcgcattcag ttcttcctga
    76501 ctcatttgtt caaacggcga aggttccggc gtaaatccgg cggtaacatc tggtgtaaaa
    76561 tgccacgctg gcttttcgcc cgtccctaat actttttcgg tcatcaggcg gcgatctatc
    76621 gtcatactta atgccagacg aacgcgctga tctgccgtcg gccctttttg cgtgttaaac
    76681 gcataataat aggtcccgag ctgcggcggc gtataaacct gccccggaat atccttcaac
    76741 agcttctgat acatattttt cgggaaggat tcggtgatat caatatcccc cgcgaggtaa
    76801 cgcttagtgg ctgcggattc ctgattaatt ggcaggaagg tcactttttg cagtaccgtt
    76861 ttggcgttat cccaataatg ggtattcggt accacgacca gtttttcatt gactacgcgt
    76921 tctttaagaa cataagcgcc attgccgatc agatttccgg gtttcgtcca ctctttaccg
    76981 ctttctacgt tggctttttg caccgggaag aaggcaaagt tagcggttaa attcacaaac
    77041 cacggcaacg gtttatcaag ctgaattttc aaggtatggg catcaactgc ggtgacgcca
    77101 agctggtcag gcgtagcttt accatcaata atcgcctgtg cgttgttgat tcccgccagc
    77161 gcggcaaacc atgcaaatgg cgatagcgtt ttcgggtcca ccagacgttg ccagctgtag
    77221 acaaaatctt gcgccgttac cggtgtgcca tccgcccatt ttgcgttatc gcgcagggta
    77281 aaagtccaga tacggttgtc attacttttc cactgagtcg caacgccggg gacaatctcc
    77341 cctttctcgt tctgattcac cagtccttca aacagatcgc gaatgacctg aatctctggc
    77401 aggcccacgg ctttagcggg atccagcgac gcaggctcat ctttaatgtg gcgcaccagc
    77461 tcctgcttct ctgccagtac tgtgccgctc ggaacttctg cagcatacga aagagaaatg
    77521 ctgctgacca acagcgcaca acacgtgact gaaacagagt gcttcataag tttccctcta
    77581 accgacatta aaattgatgc gtaattattt gtttcgcctt aaagaaatgc aaacaacttc
    77641 catcaaaaaa gtgacacagg aagcataaaa tgttttatgt atcaatatat taaatcagat
    77701 ttagtgtaaa taatcgacgc caaaatgcgg caaagcacat agaacaccga acactacgtt
    77761 caggcttcgc acgttcaaac cagttttcgg tatcaaaatc agcatctgtc gccattgtac
    77821 gaagaataaa aaataaccaa aacgttgaaa ggaactgaga atacgttaag agagaaatta
    77881 aattaatgta tcagcaagta gtgctaaaca gcacctttat acttcagcct atctataaat
    77941 aatgagaatg ccgagccagc atgtcgacgg tgtggatagt acaggtgata accgggtaaa
    78001 tctggtgtga atttatccaa aacacgtatc agtttttttt ctttaatagc ccgctcaacc
    78061 tgatcataag gtagatacgc caatccatgc ccatcaattg cagcatcaat gatcaggtct
    78121 atcgtattca gtaaaagctg accttccatg cgaacacgaa cttcacgtcc accgcgtatt
    78181 aatctccagc gatttgctgt acccgatgtg ggaagataca aattaattgc ctgatgatct
    78241 attaattgtg acactgacgt tggaacactt cggcgagaaa aataatccgg tgagccaaca
    78301 atagccattg gtatatctgg cccgattcga atggcgatca tatctttatc catctcccca
    78361 cccagacgga cgcctgcatc aaaacgttca gaaacgacat cggtcaaacc ataatcaatg
    78421 gtgagctgaa tatcaatttc aggatgcgat ttcaggaatg tgcgcattgc tggtaacaat
    78481 atcgtttttg ctgcatgttc tacagtagta atacgtattg tcccggatgg gcggttctgc
    78541 agatcgctca gggatgccat ggctgaatct atgtcatgca acatagggcc aagaacagac
    78601 aaaagatgct cgcccgcttc agttggaaca acgctgcgcg tggttcgcgt cagaagccgc
    78661 aatcccaatc gttcttctat acgacgcact atctggctta aagctgactg cgccatgctc
    78721 aggcgggctg ctgcacgagt gaagctacgc tcctctgcaa cgacgacaaa cgccatcaga
    78781 tcagcgattt cttcacgctt cattcagtat ctccatgcaa tctattaatc atttaaatga
    78841 taggtctaat catgattttg ggtctaatca acttaatgat caatatctat agtgatatca
    78901 ccaaccgtta aaacgaaccg acaactccat gaggaacgga atgatgaata ataaagtcag
    78961 cttcactaac agcaataatc caaccatctc tttgtctgca gtgatctatt tccctccaaa
    79021 atttgatgaa acccgtcagt atcaggcaat tgtgttatct cacccaggtg ggggcgttaa
    79081 agaacaaacg gccggaacct atgccaaaaa actggcggaa aagggatttg tcactattgc
    79141 ttatgacgca tcttatcaag gtgaaagtgg cggcgagccg cgtcagttag aaaacccata
    79201 cattcgtact gaagacatca gtgccgtgat tgattacctg acaacgcttt cttatgtcga
    79261 caatacccga attggtgcga tgggaatttg tgccggtgca ggatatacag ccaatgctgc
    79321 aattcaggat cgccgcatca aggccatcgg taccgttagt gcggtcaata ttggttcgat
    79381 gttccgtaat ggttgggaaa acaatgtgaa atctattgat gccctgccat acgttgaagc
    79441 gggttcaaac gcccgaacca gcgatattag cagcggagaa tatgccgtta tgccactggc
    79501 accaatgaag gagtccgatg cgccgaacga agaactgcgt caggcctggg agtattacca
    79561 cacccctcgc gcgcagtatc caacagcacc gggttacgct actttgcgca gccttaacca
    79621 gattattacc tatgatgctt accatatggc ggaagtgtac ctgactcagc cgatgcagat
    79681 tgtggcgggc agccaggcag ggagcaaatg gatgagtgac gacctgtacg atcgggcatc
    79741 aagccaggat aaacgctatc acattgttga aggcgcaaac catatggatc tttatgacgg
    79801 caaagcctat gtggctgaag caatttcagt attagctccg ttctttgagg aaactctgta
    79861 atgaaacata ttcaaatccg caatagtgat atggcctggc atattgctgc caatattcag
    79921 tttccaccaa attttgatga gtcaaaacag tatccggcga ttatcagcgt ccatcctttt
    79981 ggcagttgta aggagcagac gtctggcaat atctatggca aagcattggc tgaaaaaggc
    80041 tatctcgttc tcgcttatga cgccagtttc cagggagaat ctggtgggga gcctcgctgg
    80101 atagaagatc cgacacagcg cgtagaggat attagccgtg ttatcgatta tgcagtaact
    80161 ctgccatatg ttaatgctga acgtattggc gtgctgggag tgtgtggggg tggtggctat
    80221 gccattaatg ccgcgctgac agaaaaacga attaaagcag tagtgagtat tacgggtgtt
    80281 aatatcgggc gtctttttcg ggaaggtttc agcaattacg atccgattgg cgcattgaat
    80341 gcaatggcgt cgcaacgaac caaagaggct cgtggtggag aattacagat caacgaatta
    80401 cttcccgcca gcctggatgc agcaaaagcc catggactga ccgaacgcga tgtttatgaa
    80461 gcaactgact actataaaac accacgcggg caacaaccgg gaggtgcgac aaaaatgttg
    80521 ttctcacatg cacagaaaac gctggcctgg gatgccttcg catttactga agtgttactg
    80581 actcagccgg tgatggtagt agttggtgaa aaagtcggtg ccttcggtgc ttatcgtgat
    80641 ggactggaag tctatggccg ggcgatggtt tcgcaggatc gtcagttagt ttcattaccc
    80701 gatttctcac attatgaact gtatgacaaa ccggaagcag ttcaggaggc gctggaaaaa
    80761 gttattcctt ttttcaacac ccatctggga taagtttcat gagaaatatt atgaaaaatg
    80821 tactgattct tggtgctggc ggtcagatag cccgccatgt gattaatcaa ctggcagata
    80881 aacaaaccat taaacaaact ttatttgccc gtcagccagc aaaaatccac aaaccctatc
    80941 caactaatag caagattatt atgggagatg tactgaatca tgcagcactg aagcaggcca
    81001 tgcagggaca ggacgttgtg tatgccaacc ttacggggga agatttagat attcaggcaa
    81061 attcagtgat cgctgcgatg aaagcctgtg atgttaaacg tctgatcttt gttctttccc
    81121 tcggtattta tgatgaagtt ccaggaaaat ttggcgaatg gaataatgca gttattggtg
    81181 agcccctaaa accattccgc agggctgcgg atgcaattga ggcttctgga ctggaatata
    81241 caattcttcg ccctgcctgg ctgactgatg aagatattat tgattatgag cttacctcac
    81301 gcaatgaacc attcaaaggt acgatcgtat cacgtaagag tgtcgcagca ttaatcacag
    81361 atatcatcga taaacctgag aaacacattg gtgataatat aggtatcaat caacccgtga
    81421 ctgatggtga caagccatta ttcatgtaaa tcaattacat agttaccatc gtaatgtact
    81481 atgtgcagta cgatttatta aacttattaa ggtgaactga tcaatccgat gcgattaaca
    81541 tgtgtagaac cagtaagaat aaaatacgcc atgtatataa cattacgact tacatctaag
    81601 tcagcataat tctaatatct gcacatcaat aaaaatgcaa acaacttccg gcaaaaaagt
    81661 gatacaagat cgatgacata gcgttgaaga aaaggatttg ataatccgca aaatttgcac
    81721 aacactgcct gtaatacttc ctgaaccaga gacgatcatg accgtaaacc gcccacgcgc
    81781 cgaacgcggc gcatttccgc ccggaacaga acattacgga cgttcattat tgggcgcgcc
    81841 gttaatctgg tttcctgccc ctgcagccag tcgcgaaagt ggtttgattc tggctggcac
    81901 tcacggtgat gaaaactctt cggtcgtcac gctctcctgt gctctgcgga ctttgacacc
    81961 ttctttgcgc cgccatcatg tggtgctgtg tgtgaatcct gacggctgcc agctggggtt
    82021 acgggccaat gctaatggtg tggatttaaa cagaaacttt ccggcgacga actggaagga
    82081 aggtgaaacg gtttatcgct ggaacagcgc cgctgaagaa cgtgatgtcg tattactgac
    82141 aggtgacaaa cctggctcag aacctgaaac ccaggcgctg tgccagctta tacaccgcat
    82201 tcaacctgcc tgggtggtct cattccacga tccactggcc tgtattgaag atcccagaca
    82261 cagcgaatta ggcgaatggc tggcccaggc gtttgaattg cctctggtaa ccagcgtcgg
    82321 ttatgaaacg cctggctctt tcggcagttg gtgtgcagat ctcaacttgc attgcatcac
    82381 cgcagagttt ccgcccatct cctccgacga agccagcgaa aaatatctct ttgcgatggc
    82441 aaacttgctg cgctggcatc ctaaagatgc aattcgcccg tcgtgaactg aagcgccggt
    82501 tccacatcta ccgccagcca ggtcgatccg tcaagatcgg cgaaactgac ctgcggcacc
    82561 agcggtaaag cggcgctaat ggcacgagag gtacacaaca tgcagcccag catcagactg
    82621 aaaccttgtg cacgcgcttc agtcgccagc gccagcgctt ccgtcagacc accggtttta
    82681 tcgagcttaa tgttaaccat ctcatagcgc cctttcagcg ccttcagatt gctacgtgta
    82741 tgacaactct catcagcaca aatcggcaac ggatgaataa aattctccag cgccgcatcg
    82801 tcctgcgcag gaaggggttg ttcaagcatc gcaacgccta aatccgccaa tagctggcaa
    82861 cgcgccgcca acccttctgc acgccaggat tcatttgcat caacgatcag cgtcgcatcg
    82921 ggcacagctg tgcgaattgc caccatccgc tcactaataa gatggttatc cagcttcact
    82981 ttcagtaatt tcgcgcctgc ctgccagagt gttgatgcac tattggccat ctgatcaggc
    83041 gtaccgatga ctaccgtctg tgcagtgata actgtctcgg gaagcgttat gccgatcaaa
    83101 tcagccagcg attgctgctg ttttcgcgcg gccagatccc acaatgcaca atccagcgca
    83161 ttacgtgctg cgccggcagg gagaattttt tgcaactcct cccgtgtcag ccctttctct
    83221 aattgcgaca cgacgctcat aatttgcgcc attaccgagg catcactttc tccataacgc
    83281 ggatacggcg tgcattcgcc ggtgccttta ataccctctt cttccagttc aaccaccacc
    83341 acgcgcgctt cactgcgact tccccgggca atcacaaacg gggtatgtaa gggccaggct
    83401 tcctcaaata ccttaacggt tctcatcttt actccttgtt gtcgcggtgg tctgcggatt
    83461 aagcgcaaat agcgtttgct gtgttattga cagttagcat aaactaggtg tgacgttaac
    83521 tatatgtaaa caggaaagat aatcatgtca caaaccgttc atttccaggg caacccggtt
    83581 acagtcgcca attccatccc gcaggcgggt agcaaagcgc agacttttac tctcgtagca
    83641 aaagatctgt ctgacgtcac cctcggtcag tttgcgggta aacgcaaagt gctgaacatt
    83701 ttcccgagta ttgataccgg agtttgcgcc gcatcagtac gtaagtttaa ccaactggca
    83761 accgagatcg acaacaccgt tgtgctgtgt atttctgccg atctgccgtt cgcccagtct
    83821 cgtttctgcg gcgcagaagg tctgaacaac gttatcaccc tctccacttt ccgtaacgct
    83881 gaattcctgc aagcctacgg tgtggcaatt gctgatggcc cactgaaagg tctggcagcg
    83941 cgtgccgttg tggttattga cgaaaatgac aatgtgattt tcagccagct ggtggatgaa
    84001 atcactaccg agccggatta cgaagcagct ctggctgtac tgaaagcata atttaatatg
    84061 cctgatggtg ttgcaccatc aggcatattc gcgcttactc ttcgttcttc ttctgactca
    84121 gaccatattc ccgcaactta ttggcaatcg cggtatgtga aacgccgaga cgttttgcca
    84181 gtttgcgcgt gctgggataa ttgcgataaa gctgggttaa taccgagcgt tcaaaacggc
    84241 tggtgatttc gtccagcgaa ccttccatcg catcttcgcc cacggctacc gttgcggcgt
    84301 cataatccgg caacaaaata tcctgtggac gcagctcata accgtccagt tgtgtcagtg
    84361 cgcgatagat agcgttcttt aactgccgca catttcccgg ccacgcataa cgcgtaagta
    84421 cagtattcag gtcagcggcc agtttcggac gcggcacgcc ctgctcgtcg gcaaagcggg
    84481 cgacgaacag ctcagttaac ggcatgatgt cctgcggaca gtcacgtagc ggcggcagat
    84541 tgagcgtcaa cacgttcaga cgataataga gatcttcacg gaacatgcct ttttgcacca
    84601 gttcgaccag attcttctgc gtagcgcaaa tcacccgcac atcgacatgc acctcatggt
    84661 cttcgccaac ccgacggaaa gtgccatcat taaggaaacg cagtaatttc gcctgcatcc
    84721 gtggtgacat ttcccctatt tcatccaaca gcaccgaacc accgttcgcc tgctcaaaga
    84781 atcctttctt cccttccgga gcatgaccaa acagttcact ctcgaccgca tcttccggta
    84841 tagacgcaca gttcagcgcc aggtaaggtt tgcccgctct ggggcttgcc tgatggcagg
    84901 cgtaggcaaa gagatcttta cctgtacctg tgtcacccgt aatcagcagc ggcgcgctta
    84961 gcatcgccag tttctgcgcc tgttcgacaa catgcttcat tttcgggctg acggcgacaa
    85021 tttgactgaa ggcgctgacg tcctgggcgg cgacattttg caactggcgg cccatacgaa
    85081 tcgttgatcg caacatcacc accgcaccgg tcaggacgtg ttgatcattt tcatcctgaa
    85141 gataaacagg cgtaatctcc atcaggaaat tctgcccatt aataacgaca tgctcgttat
    85201 gggaatcttg cggttcgctt tccagccaac gtaaaaaatt aaagccgtta atcaattgtg
    85261 cggcggtatg gttgcgcagg cgatccaatt tttgcgcaaa aagctgacag ctcgccgggt
    85321 tcgccatatc cactttgctt ttcatatcga cagagagcac aggttcgggc aacgcctcca
    85381 gtaacgcgct caacgccaga tgctcacgtt cggaaggcat ccacgggaca gtacgcacat
    85441 cggtaacacc tgcaatacgg cgtatttcgg ccatcagact gctgaaactc tcaaactcca
    85501 gttcagcaaa attgaggtag attcgcccaa tgggatcaat ctcaataccg cgtaaatcaa
    85561 tgcctcttag cacgagtaga tcgagtaatt cgcgggtcag accgagtcgg tcttcacaaa
    85621 agacttccag acgcatggga accttcacct gaaaaaagaa caattaatat gatgatatga
    85681 cactatttgg atagcaggaa ggtttctgtc aacaatcatt gacacaaaga cggaaaaacg
    85741 ggcacttcgg cgtaaagata tccggtcagc acccgatatt gcattatttt tcgctgggcg
    85801 ttttgccttt ttgcagcgtt tctttcacct gaccgataag ctgacgacgg aaatccccga
    85861 ggcgaggttt gtcatcgtca atccacggca gcgggcggca aagctccata gctttaatcc
    85921 cgagtcgtgc cgtcagaagt ccagcaccaa ttccctgagc tgcgcgggta gacaaacgag
    85981 cagcgagatc ttgcgacatc cagtccatcc ccacttcgcg caccagttcg ctggctccgg
    86041 caaaagcgat attcagcaat accagcttaa acagacgcaa acggctgtaa taccccagtt
    86101 caatgccata cagcgtggcg atgcgattaa ttaaacgcag attgcgccag gcgataaacg
    86161 ccatatcgac caacgccagc gggctgaccg caatcatcaa cgttgattcc gccgccgaac
    86221 ggctgatttc gcgccgcgcc tgggcatcta aaactggctg gaccaaatgc gcatacaaac
    86281 tgaccacttc acggtcgttt tgcgtttcat ggattgaggc ataccagcgt tgcagcgctg
    86341 gatgagactg atcaataccc gcctgctgcg ccagtttttc gcaaaatgcg cggcctttgc
    86401 ccgtgccgtg gctgtgcaac aaatcgcgcg cttcgtcgcg ttcatgggcg cgctgtcgca
    86461 agcgccataa gcgccgccac tctgttacca cagaacctac gccagcgccg ataatcaatg
    86521 ccccagcggc acatccaccc agcgccaccc agtcctgagt ttgccaggca ttcattgtcc
    86581 actgtacacc ctgcccgaca acgcttgcgc caaacagagc cagcccgccc atcaccattt
    86641 tgcgccacag gctgcgtttc ggacgtaacg ctgcatccat taccgcttca acttgcccct
    86701 cttcttcagg cgcttcatcg agcgtggccg gggcaaaatt ttgcgcctga ttttcgtcaa
    86761 aggtctgctg cgccctgaat tttggattct gatcgacctc cagcggaccg tcgaaatcaa
    86821 tacgtggttt taacggttcg gtcatcgcaa tttatctcct attaaaaatt caagcgcggc
    86881 atcaagacga atatgcggca gcggtttgtc gacatccatc acctgcgggc gaaacgcttc
    86941 aaactggaac ccttgcttgt cccagaacgc ctgaccgggc aaacgcgcag gaacttcacc
    87001 gggataaacg gtgagtggcg caccatcgct gagccggtta ccgcgcaatg ccggaatttt
    87061 ctcgccgttg acgtcaataa tgccgctggt ggtcgcctgc accgaggcca gccccaggca
    87121 atccatacta atcccttcaa acgccgcatt ttgccaggca tcctgaatca gttgttgcag
    87181 taatgaaacc atattggcgt gctgatcgtt ggtcacatgg tccgctttag tggcagcaaa
    87241 caatagctta tcgataaccg gcgaaaacaa acgccggaac aaggtacgct gcccgtagtg
    87301 gaaactttgc atcagctgcg tcagtgccag acgcatatca ttaaatgcct gtggcccact
    87361 gttgagaggt tgcaggcaat caaccagcac aatctggcgg tcaaagcgca gaaaatgatt
    87421 cttatagaac cccttcacca ccttctcgca gtaataatta aaccgctcgc gcagcattcc
    87481 ggcattggtg tgcttatcgg cctgcgccag tttggactcg ccccaggcat cgacatccgg
    87541 ccacgggaag aattgcagcg cgggcgcacc tgccatatct cccggcaaga caaagcgccc
    87601 aggctgaata aagtgcagcc cctgctgttt acagtggtgg agataatcgg tccacgcggc
    87661 ggcaatgtct gccagccggt tttcgtcggc aggcgctagc gggtccagcc cttcgcacat
    87721 cattcgccat ttgaccgacc attcgccgcg ctgaccattg agtaagcccg tcatctggcg
    87781 tgaccagctt aaatagtcct gcgccagcat cggcaggtcg agcaaccatt cgccgggata
    87841 atccacaatc tccagataca gcgtggaggt gtccttgaag tggcgtagca gcgaatcgtt
    87901 cgatttaaaa cgtagcgcca ggcggatttc actgacgccg cgcgttggcg tcggccaggc
    87961 gggaggatcg ccatataact gcgccagtcc ttcatcatat gtgaagcgcg gaatgccaaa
    88021 gtcacgctgg ggaatgcgtt tcacgcccag caggcgctct tcacgcaccg cacttaacag
    88081 cggcaaacgt gctccggcgt gaatattaag caactgatta accatcgcgg tgataaacgc
    88141 tgttttgccg ctgcggctaa gtccggttac agcgaggcgc agatgtctgt cgacaccccg
    88201 attcaccagc gcattgagtt catttttaag tcgcttcatt gctgtcctgt gctactctga
    88261 agaccagaaa aaaaatcatt cgtaaggata tgaatgtggg ggccaggcca ttgattatca
    88321 actgcccgtg ttgtcatcga ccggcgaact ccataagatg tgcagcattc acgttcggac
    88381 ggagttcctg acctatgtcc cctactattt atgatattgc cagggttgca ggcgtatcaa
    88441 aatccaccgt atcacgcgtg ctgaataagc aaactaatat ctccccggaa gcgcgcgaaa
    88501 aagtgttacg ggccattgaa gaattacagt atcagccaaa caagctggcc cgcgcgctga
    88561 cctcttcggg ttttgatgcc attatggtga tttctacccg ttcgaccaaa actacggcgg
    88621 gtaatccgtt tttctctgaa gttctacatg ccatcaccgc caaagcggaa gaagaaggtt
    88681 tcgacgtgat attgcagacg tcgcacaacc tggcagaaga cttacaaaaa tgtgaaagca
    88741 aaattaagca gaaaatgatt aaaggcatta ttatgctaag ttcgccagcg gatgagtcat
    88801 tttttgccca acttgataaa tacgatattc ctgtcgtggt gattggcaaa gttgaaggtc
    88861 aatatgccca tgtttattct gtcgataccg ataattttgg cgacagcatt gcgttgaccg
    88921 atgcattaat tgaaagtggg catcaaaata ttgcctgcct gcatgctccg cttgatgtcc
    88981 atgtttcagt ggatcgggta aatggttata agcagagcct ggctgcgcat aatattgcag
    89041 tgcgtgatga atggattgtt gacggcggtt atacccatga aacagcactg caagccgcgc
    89101 gggaattatt aagccagtca ccgttgcctg aggcagtgtt tgctactgac agcctgaaat
    89161 taatgagtat ttatcgtgcg gcagcagaga aaaatattgc tattccgcag cagttagcgg
    89221 tggtgggtta tagtaatgaa acgctgtcgt ttattttaac gcctgcgcca ggcggtattg
    89281 atatcccaac acaggagtta ggtcaacaaa gctgtgagtt attattccgc ttaatttctg
    89341 gaaaaccgtc accacagaat attaccgttg ccacgcatat gacgctgaaa taaataccag
    89401 ctaccttgcc tgaatgccgg atgcgatgct agcgcatctt atccggccta catttatagt
    89461 tcggataaag tactcgtgcc gcatccggca atttagccca ttatcagaac gagtaattta
    89521 cgccgacacc tgcataatgg aatttatcgc tgtcgccttc gtcgtgatcc tgccactcaa
    89581 acgcgtattc cagcgaaacg gaaagcccgt tctggaaatc ataaccgtaa aataaaccta
    89641 cacggttaaa atcatggcct tcacgttcaa tatcatcctg ccagtcccag ttactccagc
    89701 gatccagccc aatgcgcgta tacggcgtca ccgagtggtt gccgagcgtc agcggcaaat
    89761 aggcgcgaat ttcttgcgta gaaaactcac cgttattgcg gctgtcgtcc atattaaagc
    89821 cgcgctcgag ataatagttc actcgcaagg caaccgtttc gttgaaggta tattgcaggc
    89881 ctgtttccgt ttcgactcgg gtatcagcgt aaccggtcgt gttcagatcg ttggcaaatt
    89941 tatacatcga caaccaaccg ttgaaacgta aatcgtcagt cagtttcaca tcccagtctg
    90001 gcgcgatttt ccagcgctgc atattcgccg tgtctttacc cggttcatca acgtagtgat
    90061 aaccataatt acggaaaccg ccggtcaggc cgaaactgaa atcatcgttt tcgaggaact
    90121 gataatgcac ctccagctcc gggcgatcaa accacgttcc acgtttaccc gcgctataat
    90181 ctaccggccc ttcctgataa taggccagag caattctcca cggcccgttg gcggcattaa
    90241 aatagactga aggctccgcc agcccatcca tatcttcgcc ataaccctcg acgttttcta
    90301 tttcgtacat cgcgccgata ttaaagtgcc agtcgttcct ttcctcggcc tgtgcgcagg
    90361 ccattcccgc acacatcacc agtgcggtac agggtaataa ctttttcatt attgttcctt
    90421 gtattattct gggggaatgc cttccccctg tatttagcga attgctattt ccgtttctgc
    90481 atcaaagaaa tgacatttcg tcatatcaaa atgaatagtg atattttctc ctgcatgata
    90541 atcatttaac gcaccagcac ggaccactaa ctcgtgcccc ccaaccgtgg tgtagagcat
    90601 aaattccgca ccggttaatt ctgccacgct aattttggcg gaaatgttat tttcctcttg
    90661 cgcgtccgga tgaatatctt ccggacgtat tcccatcacg atgggcttat gcaaacttcc
    90721 ctgtgttttc agaaccgcta atttctcttc gggaatggtt aatttaagcg tttccgtaac
    90781 gaatttatcg ccatcgatcg tgccacgaat aaaattcatc gccggtgatc caataaatcc
    90841 ggcaacaaac atattcgctg gttggttata aaccgttttc ggcgcaccaa cttgctgaac
    90901 aatcccatcc ttcataatca caatccgcgt cgccatggtc atcgcttcgg tctgatcgtg
    90961 ggtcacgtag atcatggtgg tattcagctt ctggtgtagc ttgctgatct ctgcgcgcat
    91021 ttgcacgcgc agcttggcat caaggttaga gagcggttca tccattaaaa acacgcccgc
    91081 ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt tgcccgccgg aaagcgcgcc
    91141 cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc gccgcccagt tcacccgctc
    91201 atcaatcacc tctttggcga ttttttgcat cttcaggcca aacgccatgt tgtcgtaaac
    91261 cgtcatatgc ggatacaacg cgtagttctg gaacaccatc gctatattgc gtgctttggc
    91321 tggaacgtca ttcattcgtt tgccgtcgat caacagatcg ccgccgctga tctcctcaag
    91381 cccggcaatc atgcgcaggg tggtcgactt accgcagccc gacgggccga caaacacgat
    91441 gaactcttta tcggcaattt ccaggttgaa gtccttcacc acatgcacct ggttatcgta
    91501 gattttttga atatgttgta acgaaagctg agccatgttg attcctttgc tatacgtttt
    91561 gccagaaggc cgataaccgc gaccaggtca gagagtccgt tgaaggcaac agtaattgcg
    91621 ccccggttaa gcccgcgccg atccccaccg agcgcatacc gctggcgtta atggcgtcaa
    91681 tgcccgcctg cgcatcttca atgccgatac atgcctgcgg cggcacgccc agcccggcac
    91741 aggcggcgag aaagatttcc gggtccggtt tcgagttttt aagttgggaa gcatccgcgc
    91801 agaaggtgaa aaactcgcgc agctccagcg ccgctaaaat cgtcggcgca ttcagggaga
    91861 cagaagccag cccaaccggg atctgctgtg cacggagatc tgccagcaaa gagcgaatgc
    91921 cgggtagaac agcgctgacc gtcaactcgc gcagtgagtg gacatagagc agatttttgc
    91981 gatacgccag ttgcgccctc tcctgcgagt taaagtcgcc ctctttgccc ccgtgttgca
    92041 gaatgcgccg cagagactca tcgcggctga tcccttttag ggattcgtta aactgcgcat
    92101 caatgctgat gccaatttca gcggcaatct gctgccacgc ctggaaatgc agatgcgcgg
    92161 tatcggtgat tacaccatcc agatcgaaaa ttaccccttg cagtttcatt catcctcctg
    92221 atgtgtggta gcggtcccat tgaagggcaa aataaaatca cccaaacaga aaacagattc
    92281 ttcagccacg gatataagct gaccgttcaa acgcagtgaa acgggcgcag aagttcgaat
    92341 cgcaatacgc tgcgcgtcaa gagtgacctg taattcgcag ccctgccaga acagagggaa
    92401 agacaactgt tgccactgct caggtaacgc cggattgaga tgcaattcac cgtcacgcac
    92461 gctcaccccg gcaaaaccct gaatcgcccc cagccagata gcgccagttg cggcagcatg
    92521 gataccatca tcacaactat gcggatcagc accaagatcg atttcagtcc cctcgcgcca
    92581 gaactgataa ctttgggtca gcaggccaca gcgtgcggca acaatgccgt ggattgcttt
    92641 acttaatgac gagtcgtgaa tagtgcgcgg ttcataaaat tgcagattgg caagacacga
    92701 tgccgctgag aactgctccg gcagcatgta attgagcatc accacatcag cttgtttgag
    92761 gatttgcatc tcgttcactt ctgcgcgtga ataatccagc agaatggttt gcttccccgc
    92821 cgccgctttg tatttcgcca ggttaatcgc cggcttagcc ataaacgaat catcctgcgg
    92881 caaaacgccg tcgggctgaa tttctggcag ccgcagttct ttaaggaaca tttcggcacg
    92941 atgaataaac gcgtcgtcgc tacagccaaa ctggcgggca atactcagcg cctgctggac
    93001 gttgtaatac gccatatagc tggtgaaggc gttattattg acatgttcgg tatattcgtc
    93061 aggcccaata acatcatgaa tttccagacg gtcgttaacc ctcaccgcgc ggctaatcca
    93121 gaactttgca gtttccagaa gtagcgccat gccttcatgc gcaatgaaac tttcatcccc
    93181 cgtggtctgc cagtattgaa taaccgccca ggcgatatcg gccaccagat ga
//
LOCUS       DAZGVQ010000003        75333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000003 DAZGVQ010000000
VERSION     DAZGVQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 75333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.003"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(40..1338)
                     /gene="eno"
                     /locus_tag="ACHLAG_000172"
     CDS             complement(40..1338)
                     /gene="eno"
                     /locus_tag="ACHLAG_000172"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFP0653483.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1426..3063)
                     /gene="pyrG"
                     /locus_tag="ACHLAG_000173"
     CDS             complement(1426..3063)
                     /gene="pyrG"
                     /locus_tag="ACHLAG_000173"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFP0653484.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3291..4082)
                     /gene="mazG"
                     /locus_tag="ACHLAG_000174"
     CDS             complement(3291..4082)
                     /gene="mazG"
                     /locus_tag="ACHLAG_000174"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFP0653485.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4153..4488)
                     /gene="mazF"
                     /locus_tag="ACHLAG_000175"
     CDS             complement(4153..4488)
                     /gene="mazF"
                     /locus_tag="ACHLAG_000175"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFP0653486.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4488..4736)
                     /gene="mazE"
                     /locus_tag="ACHLAG_000176"
     CDS             complement(4488..4736)
                     /gene="mazE"
                     /locus_tag="ACHLAG_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFP0653487.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4814..7048)
                     /gene="relA"
                     /locus_tag="ACHLAG_000177"
     CDS             complement(4814..7048)
                     /gene="relA"
                     /locus_tag="ACHLAG_000177"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFP0653488.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7096..8397)
                     /gene="rlmD"
                     /locus_tag="ACHLAG_000178"
     CDS             complement(7096..8397)
                     /gene="rlmD"
                     /locus_tag="ACHLAG_000178"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP0653489.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8454..11210
                     /gene="barA"
                     /locus_tag="ACHLAG_000179"
     CDS             8454..11210
                     /gene="barA"
                     /locus_tag="ACHLAG_000179"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFP0653490.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11442..12782)
                     /gene="gudD"
                     /locus_tag="ACHLAG_000180"
     CDS             complement(11442..12782)
                     /gene="gudD"
                     /locus_tag="ACHLAG_000180"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFP0653491.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGRTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYHKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12803..14143)
                     /gene="gudX"
                     /locus_tag="ACHLAG_000181"
     CDS             complement(12803..14143)
                     /gene="gudX"
                     /locus_tag="ACHLAG_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFP0653492.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHSAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHIGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14145..15497)
                     /gene="gudP"
                     /locus_tag="ACHLAG_000182"
     CDS             complement(14145..15497)
                     /gene="gudP"
                     /locus_tag="ACHLAG_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFP0653493.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCSFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15932..16381)
                     /gene="yqcA"
                     /locus_tag="ACHLAG_000183"
     CDS             complement(15932..16381)
                     /gene="yqcA"
                     /locus_tag="ACHLAG_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFP0653494.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16399..17181)
                     /gene="truC"
                     /locus_tag="ACHLAG_000184"
     CDS             complement(16399..17181)
                     /gene="truC"
                     /locus_tag="ACHLAG_000184"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFP0653495.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17181..17510)
                     /gene="yqcC"
                     /locus_tag="ACHLAG_000185"
     CDS             complement(17181..17510)
                     /gene="yqcC"
                     /locus_tag="ACHLAG_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFP0653496.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18132..18677)
                     /gene="syd"
                     /locus_tag="ACHLAG_000186"
     CDS             complement(18132..18677)
                     /gene="syd"
                     /locus_tag="ACHLAG_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFP0653497.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18745..19593
                     /gene="queF"
                     /locus_tag="ACHLAG_000187"
     CDS             18745..19593
                     /gene="queF"
                     /locus_tag="ACHLAG_000187"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFP0653498.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDANLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19705..21069
                     /gene="ppnN"
                     /locus_tag="ACHLAG_000188"
     CDS             19705..21069
                     /gene="ppnN"
                     /locus_tag="ACHLAG_000188"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFP0653499.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21626..22915
                     /gene="sdaC"
                     /locus_tag="ACHLAG_000189"
     CDS             21626..22915
                     /gene="sdaC"
                     /locus_tag="ACHLAG_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFP0653500.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22973..24340
                     /gene="sdaB"
                     /locus_tag="ACHLAG_000190"
     CDS             22973..24340
                     /gene="sdaB"
                     /locus_tag="ACHLAG_000190"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFP0653501.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24452..25207
                     /gene="xni"
                     /locus_tag="ACHLAG_000191"
     CDS             24452..25207
                     /gene="xni"
                     /locus_tag="ACHLAG_000191"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFP0653502.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25262..26410)
                     /gene="fucO"
                     /locus_tag="ACHLAG_000192"
     CDS             complement(25262..26410)
                     /gene="fucO"
                     /locus_tag="ACHLAG_000192"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFP0653503.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26438..27085)
                     /gene="fucA"
                     /locus_tag="ACHLAG_000193"
     CDS             complement(26438..27085)
                     /gene="fucA"
                     /locus_tag="ACHLAG_000193"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFP0653504.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27632..28948
                     /gene="fucP"
                     /locus_tag="ACHLAG_000194"
     CDS             27632..28948
                     /gene="fucP"
                     /locus_tag="ACHLAG_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFP0653505.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            28981..30756
                     /gene="fucI"
                     /locus_tag="ACHLAG_000195"
     CDS             28981..30756
                     /gene="fucI"
                     /locus_tag="ACHLAG_000195"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFP0653506.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            30865..32283
                     /gene="fucK"
                     /locus_tag="ACHLAG_000196"
     CDS             30865..32283
                     /gene="fucK"
                     /locus_tag="ACHLAG_000196"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFP0653507.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32285..32707
                     /gene="fucU"
                     /locus_tag="ACHLAG_000197"
     CDS             32285..32707
                     /gene="fucU"
                     /locus_tag="ACHLAG_000197"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFP0653508.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32765..33496
                     /gene="fucR"
                     /locus_tag="ACHLAG_000198"
     CDS             32765..33496
                     /gene="fucR"
                     /locus_tag="ACHLAG_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFP0653509.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33540..34640)
                     /gene="rlmM"
                     /locus_tag="ACHLAG_000199"
     CDS             complement(33540..34640)
                     /gene="rlmM"
                     /locus_tag="ACHLAG_000199"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFP0653510.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34633..35028)
                     /gene="ygdD"
                     /locus_tag="ACHLAG_000200"
     CDS             complement(34633..35028)
                     /gene="ygdD"
                     /locus_tag="ACHLAG_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFP0653511.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(35047..35964)
                     /gene="gcvA"
                     /locus_tag="ACHLAG_000201"
     CDS             complement(35047..35964)
                     /gene="gcvA"
                     /locus_tag="ACHLAG_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFP0653512.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(36315..36542)
                     /gene="ygdI"
                     /locus_tag="ACHLAG_000202"
     CDS             complement(36315..36542)
                     /gene="ygdI"
                     /locus_tag="ACHLAG_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFP0653513.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            36734..37939
                     /gene="csdA"
                     /locus_tag="ACHLAG_000203"
     CDS             36734..37939
                     /gene="csdA"
                     /locus_tag="ACHLAG_000203"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFP0653514.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            37939..38382
                     /gene="csdE"
                     /locus_tag="ACHLAG_000204"
     CDS             37939..38382
                     /gene="csdE"
                     /locus_tag="ACHLAG_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFP0653515.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TVAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(38433..39239)
                     /gene="tcdA"
                     /locus_tag="ACHLAG_000205"
     CDS             complement(38433..39239)
                     /gene="tcdA"
                     /locus_tag="ACHLAG_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFP0653516.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PSQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(39316..40413)
                     /gene="mltA"
                     /locus_tag="ACHLAG_000206"
     CDS             complement(39316..40413)
                     /gene="mltA"
                     /locus_tag="ACHLAG_000206"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFP0653517.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEVYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            40622..40698
                     /locus_tag="ACHLAG_000207"
     tRNA            40622..40698
                     /locus_tag="ACHLAG_000207"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40656..40658,aa:Met,seq:cat)
     gene            complement(40773..42026)
                     /gene="amiC"
                     /locus_tag="ACHLAG_000208"
     CDS             complement(40773..42026)
                     /gene="amiC"
                     /locus_tag="ACHLAG_000208"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFP0653518.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            42258..43589
                     /gene="argA"
                     /locus_tag="ACHLAG_000209"
     CDS             42258..43589
                     /gene="argA"
                     /locus_tag="ACHLAG_000209"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFP0653519.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERITAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(43651..45477)
                     /gene="recD"
                     /locus_tag="ACHLAG_000210"
     CDS             complement(43651..45477)
                     /gene="recD"
                     /locus_tag="ACHLAG_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFP0653520.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(45477..49019)
                     /gene="recB"
                     /locus_tag="ACHLAG_000211"
     CDS             complement(45477..49019)
                     /gene="recB"
                     /locus_tag="ACHLAG_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFP0653521.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIEQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDVVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(49012..51900)
                     /gene="ptrA"
                     /locus_tag="ACHLAG_000212"
     CDS             complement(49012..51900)
                     /gene="ptrA"
                     /locus_tag="ACHLAG_000212"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFP0653522.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(52076..55444)
                     /gene="recC"
                     /locus_tag="ACHLAG_000213"
     CDS             complement(52076..55444)
                     /gene="recC"
                     /locus_tag="ACHLAG_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFP0653523.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLDSSQELDAFVDVMPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(55457..55780)
                     /gene="ppdC"
                     /locus_tag="ACHLAG_000214"
     CDS             complement(55457..55780)
                     /gene="ppdC"
                     /locus_tag="ACHLAG_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFP0653524.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(55765..56172)
                     /gene="ygdB"
                     /locus_tag="ACHLAG_000215"
     CDS             complement(55765..56172)
                     /gene="ygdB"
                     /locus_tag="ACHLAG_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFP0653525.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIADYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(56169..56732)
                     /gene="ppdB"
                     /locus_tag="ACHLAG_000216"
     CDS             complement(56169..56732)
                     /gene="ppdB"
                     /locus_tag="ACHLAG_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP0653526.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVHQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(56723..57193)
                     /gene="ppdA"
                     /locus_tag="ACHLAG_000217"
     CDS             complement(56723..57193)
                     /gene="ppdA"
                     /locus_tag="ACHLAG_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP0653527.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(57377..58171)
                     /gene="thyA"
                     /locus_tag="ACHLAG_000218"
     CDS             complement(57377..58171)
                     /gene="thyA"
                     /locus_tag="ACHLAG_000218"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP0653528.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(58178..59053)
                     /gene="lgt"
                     /locus_tag="ACHLAG_000219"
     CDS             complement(58178..59053)
                     /gene="lgt"
                     /locus_tag="ACHLAG_000219"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP0653529.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(59204..61450)
                     /gene="ptsP"
                     /locus_tag="ACHLAG_000220"
     CDS             complement(59204..61450)
                     /gene="ptsP"
                     /locus_tag="ACHLAG_000220"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP0653530.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDVTLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(61463..61993)
                     /gene="rppH"
                     /locus_tag="ACHLAG_000221"
     CDS             complement(61463..61993)
                     /gene="rppH"
                     /locus_tag="ACHLAG_000221"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFP0653531.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(62346..62492)
                     /gene="ygdT"
                     /locus_tag="ACHLAG_000222"
     CDS             complement(62346..62492)
                     /gene="ygdT"
                     /locus_tag="ACHLAG_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFP0653532.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            62678..63367
                     /gene="mutH"
                     /locus_tag="ACHLAG_000223"
     CDS             62678..63367
                     /gene="mutH"
                     /locus_tag="ACHLAG_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFP0653533.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            63436..64149
                     /gene="ygdQ"
                     /locus_tag="ACHLAG_000224"
     CDS             63436..64149
                     /gene="ygdQ"
                     /locus_tag="ACHLAG_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP0653534.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            64287..64505
                     /gene="ygdR"
                     /locus_tag="ACHLAG_000225"
     CDS             64287..64505
                     /gene="ygdR"
                     /locus_tag="ACHLAG_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFP0653535.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            64613..65653
                     /gene="tas"
                     /locus_tag="ACHLAG_000226"
     CDS             64613..65653
                     /gene="tas"
                     /locus_tag="ACHLAG_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFP0653536.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(65685..66878)
                     /gene="lplT"
                     /locus_tag="ACHLAG_000227"
     CDS             complement(65685..66878)
                     /gene="lplT"
                     /locus_tag="ACHLAG_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFP0653537.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(66871..69030)
                     /gene="aas"
                     /locus_tag="ACHLAG_000228"
     CDS             complement(66871..69030)
                     /gene="aas"
                     /locus_tag="ACHLAG_000228"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFP0653538.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDPQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRMPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            69616..70647
                     /gene="galR"
                     /locus_tag="ACHLAG_000229"
     CDS             69616..70647
                     /gene="galR"
                     /locus_tag="ACHLAG_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFP0653539.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(70654..71916)
                     /gene="lysA"
                     /locus_tag="ACHLAG_000230"
     CDS             complement(70654..71916)
                     /gene="lysA"
                     /locus_tag="ACHLAG_000230"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFP0653540.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDVGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            72038..72973
                     /gene="lysR"
                     /locus_tag="ACHLAG_000231"
     CDS             72038..72973
                     /gene="lysR"
                     /locus_tag="ACHLAG_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFP0653541.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(72960..73652)
                     /gene="ygeA"
                     /locus_tag="ACHLAG_000232"
     CDS             complement(72960..73652)
                     /gene="ygeA"
                     /locus_tag="ACHLAG_000232"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFP0653542.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(73781..75199)
                     /gene="araE"
                     /locus_tag="ACHLAG_000233"
     CDS             complement(73781..75199)
                     /gene="araE"
                     /locus_tag="ACHLAG_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFP0653543.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT        18379 a        20355 c        18471 g        18128 t
ORIGIN      
        1 gcccggaggc tggcattttt aaatcagata aagtcagtct tatgcctggc ctttgatctc
       61 tttacgaccg ttgtacggtg ctttttcgcc cagagcttct tcgatacgaa tcagctggtt
      121 gtatttagca acacggtcag aacggctcat agaaccagtt ttgatctggc ctgcagcagt
      181 accaacagcc aggtcagcga tggtagcgtc ttcagtttcg ccagaacggt gagagataac
      241 tgcagtgtag ccagcatctt tcgccatctt gattgcagcc agagtttcgg tcagagaacc
      301 gatctggttg aatttgatca ggatggagtt agcgatacct ttttcgatac cttctttcag
      361 gatcttggtg ttggttacga acaggtcgtc accaaccagc tggattttgt cgcccagaac
      421 tttggtctgg tatgcgaaac cgtcccagtc agattcgtcc agaccgtctt cgatagaaac
      481 gatcgggtac tgtttggtca gttcttccag gaagtgagtg aattcttcag aggtgaacgc
      541 tttgttgcct tcgccagcca gaacgtattt accatctttg tagaattcag aagctgcgca
      601 gtccatcgcc agagtgatgt ctttgcccag ttcataacca gcagctttaa cagcttcagc
      661 gataacagcc agagcttcag cgttggaacc caggttcggc gcatagccac cttcgtcacc
      721 aacagcagtg ttcatgcctt tcgctttcag aacttttgcc aggtgatgga aaacttcaga
      781 acccatgcgg atggcttctt tcacagtttt cgcgccaacc ggctgaatca tgaattcctg
      841 gatatcaacg ttgttgtcag cgtgctcacc accgttgatg atgttcatca tcggaaccgg
      901 catagagtat ttgcccggag taccgttcag ttcagcgatg tgctcgtaca gcggcatacc
      961 tttagcagct gcagcagctt tggcgttagc cagagataca gccaggattg cgttcgcgcc
     1021 gaatttggat ttgttttcgg tgccgtccag gtcgatcatg atcttgtcaa tgccagcctg
     1081 atctttagca tctttgccaa tcagcgcctg agcgatcggg ccgtttaccg cagcaacagc
     1141 tttggttacg cctttaccca ggaaacggga tttgtcgcca tcgcgcagtt ccagagcttc
     1201 acgggaacca gtagaagcac ctgacggagc agctgccata ccgacgaaac caccctccag
     1261 atgtacttcg gcttcaacag tcgggttacc acgggagtcg atgatttcac gaccgatgat
     1321 ttttacgatt ttggacatta ggttttcctc aagtcactag ttaaactgaa actccagaca
     1381 aacaacgcgt acccagggta cgcgttgccg ttctaacttt tttacttact tcgcctgacg
     1441 cttctggaac tcgctggcgg cttttacaaa gccagcaaac agcgggtgac catcacgtgg
     1501 agtagaagta aactccggat ggaactggca agccacgaac cacgggtgat tcggaacttc
     1561 gatgatctcg accaactgat catccccgga acggcccgca acgcgcagac ctgcatcttc
     1621 aatctgtttc aacagcatgt tgttgacttc gtaacggtga cgatgacgct caacaattgt
     1681 cggcgcattg tacagctggc gaaccaggct atcgtcaacc aactggcact gctgtgcgcc
     1741 gagacgcatg gtaccgccga gatcgctctt ctcgctacga acttcaacgt tgccgttttc
     1801 atcgcgccac tcggtaatca gcgccacaac cgggtactta cagtctggca caaattccgt
     1861 agagttggcg ttctccatgt tggcaacatg gcgagcgtaa tcaattaacg ccacctgcat
     1921 acccaggcaa atgcccagat aaggaatatt gttctcacgc gcaaaacgcg cggtcgtaat
     1981 catgccttct acgccacgat agccaaaacc gccaggtact aggattgcgt ccagaccttt
     2041 aaggatttca acgccgcgcg tttcaacatc ttgtgaatcg atcagtttga tgttgacgct
     2101 gacacgattc ttcagcccac cgtgtttcag tgcttcgatc actgatttat aagcatccgg
     2161 cagttcaatg tacttgccga ccataccgat ggtgacttca cttaccgggt tcgcttcttc
     2221 gaagataacc tgttcccatt cggacagatt cgcttccggg cagtttaagc tgaatcgttt
     2281 acaaatataa tcgtccagcc cctgagattt caacaggccc ggaattttat agatggaatc
     2341 gacgtctttc agagaaataa ccgctttttc cggaacatta cagaacaatg caatcttcgc
     2401 acgttcgttc gccggaacag cgcgatctga acgacaaatc aggatgtcag gctggatacc
     2461 gatggagagc agctctttta cagagtgctg agtcggtttg gttttgactt caccagacgc
     2521 tgccatgtac ggcaccagcg tcaggtgcat aaacagagtg tgctcacggc caatttcaac
     2581 agccatctgg cgaatcgctt cgaggaacgg caaggattcg atatcaccta ctgtaccgcc
     2641 gatttctacc agtactacgt catgaccttc gccaccttcc agcacgcgct ctttgattgc
     2701 gttagtgatg tgcggaataa cctgcacggt tgcgccgagg tagtcaccgc ggcgttcttt
     2761 acgcagaacg tcagagtaga tacgacccgt ggtgaagttg ttgcggcggc tcattttggt
     2821 acgaatgaaa cgctcgtagt gccccaggtc caggtcggtt tcagcgccgt cttcagtaac
     2881 gaacacttcc ccgtgttgga ttgggctcat agtacctgga tcgacgttga tgtacggatc
     2941 cagtttcatg atggtcacat tgaggccacg ggcttcaaga atggctgcga gggaggctgc
     3001 ggcaatgcct ttacccagag aggatacgac cccgccggtc acaaaaatat agttcgttgt
     3061 catgctgaac ctgagaagtt aggttgaaaa gacgatggaa taaccaggac gggaaagcag
     3121 tatacccgaa catgacctgt gccacaaact ttcattatcc ctcctcttcg ccagcgcact
     3181 attgaaatca ggagtgagaa aatagcccct ttggggtaaa tgtttttgac gcaaatcaag
     3241 cgcttgtcat ttaaaaaatg acacaaatgg cgcttgaccg cgtaattccc ttagagatca
     3301 atttcctgcc gttttacctg ttgccagact tcttccattg tttcgaggtc aacacctgtc
     3361 atttccagtc cacgcgcggc aacaatacgc tccacttcgc gaaaacgacg ctcgaatttt
     3421 tcgttcgctt tttgcaatgc gatttctgct ttcgtcccta aatggcgagc cagattaacc
     3481 gtggcaaaca gcaggtcccc catttcctcc tccagtttag cctggtcgac aacagcctgc
     3541 cgcgcttcgt acatcacctc gtcgatctct tcgtagactt tatcgactac cggaccaagc
     3601 gtcgtccaat cgaagccaac gttggcgcaa cgtttctgga ttttttgcgc acgcattaaa
     3661 gccggtaaac tacgaggaat atcgtccagc gccgaatgct gcgctttctg cgcgcgctct
     3721 tcggttttga tttgctccca acgggcaagc acttcactac tgttttcggc agaactatca
     3781 gcaaaaacat gcggatggcg acgctctaat ttatcgctaa tagcagcgca aatatcatta
     3841 aagtcaaagc gcccttcttc ctgagccatt tgcgcgtaaa acaccacctg gaatagcaga
     3901 tcgcccagtt caccgcgaag atcgtcaaaa tcttcacggg cgatggcgtc cagcacttcg
     3961 taggtttctt caagggtgta aggcgcaatg gtggcaaatg tctgctcttt atcccacggg
     4021 cagccgtttt ccggatcgcg caggcgctgc ataatagtga gcaaacggtc gatttgattc
     4081 attgaattgt cctgaaaatt gcgggtctgt caggtggaaa cctgtgacca gaatagaagt
     4141 gagttagtaa cactacccaa tcagtacgtt aattttggct ttaatgagtt gtaattcctc
     4201 tggggcaact gttcctttct tcgttgctcc tcttgcccgc caggcgatac tttttacctg
     4261 atcagctaac gctacgccat cacgttcctg accggataaa acaacttcga acggatatcc
     4321 ttttgattgc gttgtataag gaacacacag acacatacct gttttgttgt tgtacatgaa
     4381 aggactcagg acaacagctg gacgatgtcc agcttgctcg ctaccttttg tcgggtcaaa
     4441 atcaacccaa atcagatcgc ccatatcggg tacgtatcgg cttaccatta ccagacttcc
     4501 ttatctttcg gctctcccca gtcgatattc tcgtggaggt tttccggcgt gatgtcgttg
     4561 accagttcag caagcgtaaa tacgggctct ttacgcactg gctcaataat taatttgcca
     4621 tccaccaggt caatcttcac ttcatcatca atattcagat tgagcgcctg cattaacgta
     4681 gccgggatcc gcaccgccgg tgaatttccc caacgcttta cgctactgtg gatcataacc
     4741 ctttcctcaa accgctatca tatgtagata cagtatatat caatctacat tgtagatacg
     4801 agcaaatttc ggcctaactc ccgtgcaacc gacgcgcgtc gataacatcc ggcacctggt
     4861 tgagtttacc cagcacgcgc cccagcactt gcaggttgta aatctcaatg gtcatgtcga
     4921 tggtcgccag ttgctgtttg gtgtcgctac ggctggcaac gccaagcacg ttcaccttct
     4981 cgttggcgag aatggtcgtg atatcacgta acaacccact acgatcatta gccaccacac
     5041 ggaccaccag cgaatatccg gcggagtagc tctcacccca taccgcgtca acaatgcgtt
     5101 ctggcgcatg ggagcgcagt tccgccagtt gttcgcaatc ggcgcggtgt actgaaatac
     5161 cgcgcccctg ggtaatgaag ccgacaatct catctccagg aatcggctgg cagcagcgcg
     5221 cgatgtggtg catcaggttg ccaacacctt cgaccaccac gcgaccgtta tctttactgc
     5281 ggttttgcgg cgtgtagctt ttttgctgaa gttgcttcag cgcggcggcg tcctgctctt
     5341 cggcactcgg cttattaaat tgcgattgca ggaagttcac catctgattg agacggatat
     5401 ccccgccacc aatcgccgcc agcaactcgt cgacatcatt aaagttgtaa cgcggcagca
     5461 gatgtttttc tgcttctttc aggctgatcc ccagatgttc cagctcgtcg tcaaggattt
     5521 gccgcccagc cagaatgttt ttgtcacggt cctgtttacg gaaccaggcg tgaattttcg
     5581 aacgcccacg gctggttgtg acgtaaccga ggtttgggtt taaccagtca cggctggggt
     5641 tcggctgttt ctgggtgata atttcaatct ggtcgcccat ctgcagctgg taggtgaacg
     5701 gcacaatgcg cccgccaatt tttgccccga tgcagcggtg tccgacatca ctgtggatgt
     5761 ggtaagcgaa gtccagcggc gttgatcccg caggcaaatc aacgacatca cctttcggcg
     5821 taaagacata cacccggtcg tcaaagacct ggctgcgtac ttcgtcgagc atttcgccgg
     5881 aatcagccat ctcttcctgc cacgcaatca gtttacgcag ccaggcaatc cggtcttcat
     5941 gtcccgaacg tgcgccgcca gctgccgcgc cctctttata tttccagtgc gcagcaacac
     6001 ccaactctgc atcttcatgc atctgtttgg tgcggatttg gatctcaacg gttttgccac
     6061 ctggccccag aaccacggta tgaatagact gataaccgtt tggtttcggg ttagcgacat
     6121 aatcgtcaaa ctcatccggc aggtggcgat agtgagtgtg cactatcccc agtgcggcat
     6181 aacaatcctg taaacgctcg gcgacaatac gtaccgcacg cacatcaaac agctcatcga
     6241 aggcgaggtt ctttttctgc attttgcgcc agatactgta gatgtgtttc ggacgaccat
     6301 acacttcagc tttaacgcct tcagctttca tctcagcgcg cagatgacca acgaactctt
     6361 cgatgtagtg ttcgcggtcg agacgccgtt catgcagcag tttggcaatt cgtttgtatt
     6421 cggttggatg gaggtaacgg aagcagtaat cttccagttc ccatttcagt tgtccgattc
     6481 cgagacggtt agccagcggt gcgtagatgt tggtacactc ttttgccgcc agtacacgtt
     6541 catcttccgg cgcatctttt acttcgcgca gatgagcaat acgctccgcc agtttgatga
     6601 ctacgcagcg aaaatcatcg accatcgcca ataacatccg gcgaacgtta tcgacctgtt
     6661 cggaggaaac agaatcagtg tgcgtcgctt tcagctggcg gatcgccgcc atatcacgca
     6721 cgccgtgaat aaggttaacg accgacttac cgacgctctc acgcagcaca tcttcgctga
     6781 ctacgttggc atcagccaga gggaacagca gcgccgcccg cagcgtgtca atgtccatac
     6841 ttaatgtcga gaggatctcc accatctcaa caccacgcca caataacaga ctggcatccg
     6901 gatgcccctg cgtctgttgc agacaatacg cccaggtttc ggctaagcac tcacacgact
     6961 tctggctggt aatacccaga cttgcgatcc atttttccgg atcaaattca ccagccttat
     7021 tgatatgtgc acttcttacc gcaaccattg tcctctcctt tagggaccag acctgccgaa
     7081 atcggcaaat cgtaactatt taacgcgcga gaaaagtacc atcgattcca gatgtcccgt
     7141 gtgtgggaac atatccagca tcgccagtcg cgcaatggta tatcctgctt ttaataacgc
     7201 ttcgctatcc cgagccagcg ttgcagggtt acaggataca taaactatac gaataggttc
     7261 cagttttata atttgctgca taacacctgc ggcacctgct cgcgccgggt ccagcaacac
     7321 tttatcgaag ccgtttttcg cccacggctg ctttgtgaca tcttcttcaa gattttcgtg
     7381 ataaaacgtc acattctgta aaccattaag acgcgcattc tgctggcctt tttccaccag
     7441 cgccggaaca ccttctacac cgaccacact ggctgcttgt ttcgccaatg gcagtgtaaa
     7501 gttgcccata ccgcagaaca gatccagtac gcgatcttca ggttgcacat ccagccattc
     7561 caacgcacgc gctaccattt tttggttcac acccgcattg acctgaataa aatcgcgcgg
     7621 gctaaaagtt aagcgcaacc cgtttgagtc ataccagggc atctcaccag agacggtttc
     7681 gagtatctca ctatcggggg cgagatacag atccaggcct tcagaatgcg aaaagcgttc
     7741 cagtttttcg cgatctgccg aacttagcgg tgcggtatgg cgcaaaatca tcagcgtgcc
     7801 gctggttgcc tgtaccagtt caacatgacc aagatggcgc ataacttgta agctgcccag
     7861 gcatgccctg actttgggca gcaatgcttc aagttggggc actaaaatgg ggcattgttt
     7921 aacgtcgaca atgtcactgg agcccgcttt gcgaaacccc atctgaagtt gctgtgtttt
     7981 cggtaagtag ttcagactta aacgcgcgcg gcggcgatag ccccagggaa catcggcgat
     8041 cacttcagag acatcgtgtt tcattaatcg ggcgagtgcc gcacttttgc ttcgctgctg
     8101 taaatccacg ctggcgtgtt gttgctgaca gccgccgcat acgccaaaat gaggacagcg
     8161 tggtgtttcg cgttccgggc tatcgcttaa ccggcgtacg actttagcgc gggcatactg
     8221 ttttttatct tcagtaacag caacttccgc gttttcctgc ggcaataatc cggggataaa
     8281 taacgttttg ccgttatgtc gcgccacgcc ctgaccaaaa gagtcgaggt cgttgactga
     8341 aacggttatg atctgacgcg tcgtcgtgcg tcgttttgca gagtagaatt gcgccattgg
     8401 cgagactttc tcaatttaac agtgtgacct taattgtccc ataacggaac tccatgacca
     8461 actacagcct gcgcgcacgc atgatgattc tgatcctggc accgaccgtc cttattggtt
     8521 tattgctgag tatctttttc gtcgtgcatc gctataacga cttgcagcgt caactggaag
     8581 atgccggtgc cagcattatt gagccgcttg cagtttctac tgaatatggc atgagcctgc
     8641 aaaatcgcga atctatcggt cagttaataa gcgtactgca tcgtcgccat tccgatattg
     8701 ttcgcgcgat ttcggtttat gatgaaaata accgactctt tgtcacctcc aattttcatc
     8761 ttgatccctc atcaatgcag ctcggcagca acgtgccgtt tcctcgccag ctcactgtca
     8821 ctcgtgacgg cgatattatg atcctccgca cgccgattat ttctgagagt tactcccccg
     8881 acgaatcgcc cagtagcgat gccaaaaata gtcaaaatat gttgggatat attgcgctgg
     8941 agctggatct taaatcggtt cgcttgcagc aatataaaga gatctttatt tccagcgtga
     9001 tgatgctgtt ttgtatcggt attgcgctta tttttggctg gcgcttaatg cgcgatgtaa
     9061 ccggtccgat tcgcaacatg gtgaataccg tcgaccgcat ccgtcgcggg caactcgaca
     9121 gccgggtgga aggatttatg ctcggcgagc tggatatgct gaaaaacggt atcaactcga
     9181 tggcaatgtc gctggctgct tatcacgaag agatgcagca caatatcgac caggcgacgt
     9241 ccgatctgcg tgaaacgctg gagcagatgg aaattcagaa cgttgagtta gatctggcga
     9301 aaaagcgcgc ccaggaagcg gcgcgtatta aatccgagtt tctggcaaat atgtcacacg
     9361 agctgcgtac accactgaat ggtgttattg gctttacccg cctgacgctg aaaacagaat
     9421 taacaccaac gcagcgcgat cacctgaata cgattgaacg ttcggcaaat aatttgctgg
     9481 caattattaa tgatgttctc gacttctcga aactggaagc aggtaagctg attctggaaa
     9541 gtattccatt cccactacgc agcacgctgg atgaagtcgt tactctgctg gcacattctt
     9601 ctcacgataa agggttggaa ctgacgctca atattaaaag cgacgtgcct gataacgtga
     9661 tcggcgaccc actgcgatta cagcaaatca tcactaacct ggtggggaat gcaattaaat
     9721 tcaccgagaa tggcaacatt gatattctgg tagaaaaacg tgcgctgagt aataccaaag
     9781 tgcagattga agtgcagatt cgggataccg gcattggtat tcctgaacgc gatcaatcgc
     9841 gcttattcca ggccttccga caggctgatg ccagtatttc ccgccgtcat ggtggcaccg
     9901 gtctggggct ggtgattaca caaaaactgg ttaatgaaat gggcggcgat atttcgttcc
     9961 atagccagcc gaatcgcggt tcaactttct ggttccacat taatctcgat ctgaacccga
    10021 acattattat cgaagggcca tccacccagt gcctcgccgg taagcgcctg gcctatgtcg
    10081 aaccaaactc cgcagcagcg caatgcacgc tggatatttt aagtgaaacg ccgctggaag
    10141 tggtttatag cccaacgttc tccgcgctgc ctcccgcgca ttacgacatg atgttgttag
    10201 gcatcgcggt gaccttccgc gagccgctaa caatgcaaca tgagcgatta gcgaaagcgg
    10261 tatcgatgac cgatttcctg atgctggcac ttccttgcca tgcacaagtc aatgcagaaa
    10321 aactcaagca agatggtatc ggcgcgtgtc tgctgaaacc attaacacct acgcgcctgt
    10381 tgcctgccct gacggaattt tgtcatcaca aacaaaacac gcttttgcct gtaaccgatg
    10441 aaagcaagct ggcaatgaca gtcatggcag ttgatgacaa ccccgctaac ctgaaactta
    10501 tcggcgcatt gctggaagat atggtgcaac atgtggaact ttgcgatagc gggcatcagg
    10561 cggttgaacg ggcgaaacag atgccgttcg atttgatctt aatggatatt caaatgcctg
    10621 acatggatgg cattcgggcc tgcgagctca tccaccagct cccccatcag caacaaacgc
    10681 cggttatcgc ggtaacggcg catgcaatgg ccgggcaaaa agagaagctg cttggcgcag
    10741 ggatgagcga ttatctggcg aaaccgattg aagaagagcg attgcataat ttgttgttgc
    10801 gctacaagcc tggcagcggt atttcctctc gcgtcgtgac gcccgaagtc aacgaaattg
    10861 tggtgaaccc gaatgcgacc ctcgactggc aactggcact acgccaggca gcaggaaaaa
    10921 ccgatttagc gcgcgatatg ctgcaaatgt tactcgattt cctgcctgaa gttcgcaaca
    10981 aagttgagga acagctggtt ggagaaaacc cggaaggcct ggttgatttg attcataaac
    11041 tgcatggcag ttgcggctat agcggtgtgc cgcgtatgaa gaatctctgc caactgatcg
    11101 aacaacagct acgtagcggt actaaagaag aagatttgga gccggagctg ctggaactgt
    11161 tggacgagat ggataatgta gcgcgcgaag ccagcaaaat tctcgggtaa cggacgtctt
    11221 gtcgagtagc aatcggcttt ttatgacgct ggtttcagat tggtgacaaa gtgcctgtgt
    11281 ttatgccgga tgcggcgtta acgccttatc cggcctacaa aaccaattaa attcaatgaa
    11341 ttaaaaaatg atgtagacct gatacatagc acaacaggct attttgcgtt tagcatcagt
    11401 ctcaagccgg ctccagatag agccggtttt ggttttctgt cttaacgcac catgcatggg
    11461 cgcttgttat cgaacgtcca gccaggaatc agatactgca ttcccatcgc atcgtcacgc
    11521 gcgccaagcc cgtgtttatg atacagctca tgggctttca tcacttgatc catatcgatt
    11581 tctacaccca gccccggttt ttccggcacc tgaaccagac cgcctttgat ctcaaacggt
    11641 tctttggtca ggcgctgatt accttcctgc cagatccagt gcgtatcaat agcagtaatt
    11701 ttacccggtg cagcggcggc aacatgggta aacatcgcca gggaaatatc gaagtggttg
    11761 tttgagtgtg aaccccaggt caggccaaat tcatggcaca tttgcgccac acgtaccgaa
    11821 ccttgcatcg tccagaaatg cggatccgcc agcgggatat caacggattg cagggagagc
    11881 gtatggccca tctgccgcca gtcggtggcg atcatattgg ttgcggtcgg caggccagtt
    11941 gcgcgacgga actctgccat cacttcacgc ccggagaaac cttgctccgc accacacgga
    12001 tcttctgcat aagccagcga ccctttcagg tatttaccaa ttttaatcgc ttcgttcagc
    12061 gaccaggcac cgttaggatc gagcgtaata cgcgcctgcg ggaagcgttg cgccagtgcc
    12121 acaatagact cggcctcttc ttccccggcc agtacaccgc ctttcagttt gaaatcgttg
    12181 aagccatatt tttcatacgc cgcttccgcc aggcgcacca ccgcatccgg cgtcatcgct
    12241 tcttcatgac gcaggcgata ccagtcgcat gagtcatccg gctggctttg atacggcagc
    12301 ggtgtggctt tgcgatcacc gacgaagaac agataaccga gcatttcgac ttcgctacgc
    12361 tgttgaccat cgcccagcag cgatgccacg tttaccccca gatgctgccc cagcagatcc
    12421 agcattgccg cttctatccc ggtaactaca tgaatagtgg tacgcaaatc aaatgtctgc
    12481 aaaccgcgcc cgccggcatc acgatcggca aatgtattac gcaccagcgt cagaacgttt
    12541 ttgtattcac ccagcgttct acccaccacc agcggaatcg catcttccag cgttttacgg
    12601 attttctcgc cgccgggaat ttcgcctacg ccggtgtgac cggaattatc tttgataatc
    12661 acaatattac gcgtgaagaa cggtgcgtgt gcaccactca gattcatcag catactgtca
    12721 tgacccgcca ccgggatgac ctgcatttca gtaacaacag gcgtcgtaaa ttgagaactc
    12781 atatttatgt ccttattcag aatcagtgac ggccgaaaac gggacgttta cggtcaaagg
    12841 tccagccggg gatcaggtac tgcatcggac ctgcgtcgtt acgcgcaccg ccaggcagac
    12901 gtttataggc ctcatgtgcc ttttgtacct gttcccagtc cagttccacg cccagaccgg
    12961 gcgcatcagg aacggcaatt tttccgtttt taatctccag cggatttttg gtcaggcgac
    13021 aatcgccctc ctgccaaatc cagtgggtat cgatagcggt aggattaccc ggtgccgccg
    13081 cgcccacatg ggtaaacatc gccagagaaa tatcgaaatg gttattagaa tggcagcccc
    13141 aggttagccc ccagtcgtcg caaagctgtg ccacgcggac tgcaccggaa agcgtccaga
    13201 agtgcggatc ggcaagtgga atatctaccg cattgagcat caccgcatgg cccatttcgc
    13261 gccagttggt ggcgatcatg ttagtcgcga cgggcaagcc ggtcgcccgt cgaaactccg
    13321 ccatcacttc acgaccggag aaaccctgct cagcgccgca cggatcttcg gcataggtaa
    13381 gaacatcatt cagccctttg cacaaagaaa tggcttcatc aagcagccat gcaccgttgg
    13441 gatcaacggt aatccgcgca tccgggaagc gtttcttcaa tgcacgaaca gtgtcgattt
    13501 cttgctcgcc aggtaacact ccgcccttaa gtttgaaatc tttaaagcca tagcgatcct
    13561 gtgaggcttc cgccagacgc acaacggctt cgctgttcat cgctttctga tggcgcaact
    13621 gataccactc atggttgccc ggcgtatttt ccagataagg aagatcggtt ttggtccgat
    13681 caccgatata aaacagataa ccgaggacgg taatagcatc gcgttgcttg cctggcccta
    13741 acagttcgca gaccggaaca ttcaacgcct tacctagcag gtcaagcaag gcggcttcca
    13801 gcgccgccac ggcattaacg cgcaattcaa aagtccaggc acctttgccg aaggtatcaa
    13861 aatcggccgc ctgattacct atatgcacct gctgaaccac tttattcagg cgcgcaactt
    13921 cctggcccag caccatcgga atagcatcga caagcgtctg ataaatcacc tctccgcccg
    13981 gcgcttcacc aataccggta tgcccggcat tatcggtgag taccacaata ttgcgggtga
    14041 aatatgcgct atgtgcacca ccaatattaa gcaacatgct atcgtgcccg gcgaccggaa
    14101 tgactttcat atcagtaata acaggactgg attgtgtcgt catcttattg ccccgcgaca
    14161 ggtttcagct cgatacgctt gatatcgccc accagcacca ggtagctcag taccgcgatt
    14221 aaggcatgaa caccaacata aatcagcgcc ccattaaagg agccagtcgt gccaacgata
    14281 taaccaattg cgattggcgt gacgatgcca gaaatgttac cgaacatgtt gaacaggcca
    14341 ccggaaagac cgctgatctc ttttggcgcg gtatctgcca ttactgccca acccagcgcc
    14401 ccgatgccct taccgaagaa ggccagcgcc ataaagccga tgatcatcca ctcaacgttg
    14461 acgtagttgc agaacaccat caccatcgac agcaacatgc ccattacgat cggtgtttta
    14521 cgcgcaatgt tcagcgatcc cgtgcggcgc atcagccagt cggaaataat cccacccagc
    14581 acaccgccga taaaactgca aaccgccgga acggaagcca caaagcccgc tttcagaatc
    14641 gacatcccgc gtgcctgcac cagataaacc gggaaccagg taataaagaa gtaagtcagg
    14701 gcgttgatac agtactgacc gatataaacg ccgatcatca tccgtgaccc gagcagctgt
    14761 ttgatctgcc cccacttcac gctgaacggc actttaactt tggtgttttg ctgatccata
    14821 ttgatcagcg cgccacccgc ggcgatgtac tccagctctt tctgatttac ccccggatgt
    14881 tgatttggct cgtggatgac tttcaaccag ataaagctga tgacaatccc cagaccgccc
    14941 ataaagaaga agacgtgtga ccagcccact tcatgcgtca gccagcccat aatcggcgcg
    15001 aagatcaccg ttgcgaagta ttgagcggag ttaaaaatcg acaccgccgt tcccctttcc
    15061 tgcgccggaa accaggccgc aacaatgcga ctgttgccgg ggaaagatgg cgcttcagca
    15121 agcccgacca ggaagcgcag agtaaacagg gcaacgataa tgccgaatcc actaaagata
    15181 tcgacgaagc cttgcagcaa ggtaaacatc gaccagataa agatcgacca gaagtagacg
    15241 cgttttgaac caaaacgatc cagcaaccaa ccaccaggga tctgcccgat aacataagcc
    15301 catgagaagg cagagaacac atagcccatt cccacgggat caaggccgat atctttggcc
    15361 atttccgaac cggcaataga aagcgtagcg cggtcgccgt agttgaagga tgtgacgata
    15421 aacaacatca ccactatcca gtaacgagca ttcgtgcgtt tttccacact gctcgcagcc
    15481 tgacttaaag aactcattgt tgcactcctg aaaattcgcg ttagccacgc tcactctgga
    15541 ctgcgacata gccaggaaat cagaggtgac gtagggtgtt ttttgccgtt tttataggtc
    15601 gttcgccgaa tacggcgcgt gtttatatct ggcaatagca gtataaaaag cgcgccatag
    15661 cggctcaccg tgcaacaaca caacattaat gcgttcaatg aggcccgatt ttggcattag
    15721 ccctggacgg tggaatccac ttcacggaaa tgaaaacaag aacaagaaag gaagggttaa
    15781 aacgaagaaa taaagaagag tatgaaatgg atcgcttgac tccaggcaaa cgccagtaaa
    15841 aatccgcgct atgaagcagt ttttactggc atttgcctga aaatattcga ttcagcaccg
    15901 ctaaaacgac atttaccgct cgctgaacat atcaggacaa cagcgtgccc cactgttcga
    15961 cccacggatt tgattccgtt tccggttccg ggttctcact ggcgtcaatc aacagcattt
    16021 cgccaacccg ctgagcgctc tgttcctgca acaaggcatc gaactgtttg ccgccattgc
    16081 agaaatttac ataactgctg tcgccgagcg caatcacgcc ataacgcaga ttcggctgga
    16141 agcccagact atctttgatt ccctgaaaga gcggcacaat gctatcagga aggtcgccct
    16201 gcccggtcgt ggacgtaacc accagaacat acttatcctg atagggcagc cagtcgctta
    16261 attcaggatc ttcaaatacc gttgctttgt ggccctgtgc ggtcagaatt gcttctgctt
    16321 cttccgccac taacagcgaa tttccgtaca tggtgccgac aaaaatacca atttccgcca
    16381 taccgttttc tccctggatt aggaacttat ctctccatcc tgacccgatg gcgcactgaa
    16441 ctcaaccctt tcattttcag gaagcagacc gcgccagcca aattgtgata acgcctgcat
    16501 ccaggtgtcg tccaggcccg cgtgaatagt cagcggctcg ccagtaaaag gatgcgtcaa
    16561 tgacaactga ctggcatgta gcattaaccg ctggaggcca aaatgctcag caccgctgcg
    16621 attctggcgt aaatcgccat gtttgctatc gccaataatc ggatgacgca aatgggcaag
    16681 atgtcggcga agctgatgtt tgcgtccggt tttcggctcc agttccacca ggccgtagcg
    16741 cgtggtcggg taacgtccgg tcgctaccgg catttctacg gtcgccagac cgcgataatg
    16801 cgtcactgct ggctgcgagc ctttatcttc gcgggcaaat ttatcagcga ttttgtccag
    16861 ttcttccacc agtggataat ccagaactgc ttcttccatc aaccagccgc gcacaatcgc
    16921 atggtaacgt ttctggattt ggtgctgttc aaactgttgt gccagcagcc gtccggcctc
    16981 gctggataat cccatcaaca acacaccaga agtgggtcgg tccagacgat gagcggtaaa
    17041 aacgtgctgg ccaatctgat cacgcacggt ttgcatgacc actactttct cgtcgcgatc
    17101 cagccagctg cggtgaacca gccagccgga gggtttattt accgcaacca gccattcatc
    17161 ctgatagagt atttccagca ttagctcgca tcatccgcaa aaagagcatc cagtttttcc
    17221 agctcagcca gaataagcgc gcgttgcgga tggtccgtcg ccagcgccat ttcataatag
    17281 ggtgcaacgg caaaagcgcc cggtaacggc tgtttgttat ctaacaaatc gtgcattcgc
    17341 gggatcagca cccactgcaa ccactccagt ggttccatgg tgtccataaa gaacggttgg
    17401 gtactattaa attgatgcgg ctggggttca tcgtttcgcc agtgctgatg ttcacgcagt
    17461 aacgcttcaa gcgcctgcaa ctggagacga acgcggtcat gagtggtcat aaagcaacct
    17521 caataagaaa aactgccgcg aaggatagca ggaataaaaa aagggagcac tgtattcaca
    17581 gcgctcccgg ttcgtttcgc aacattccag ctacttttgt tgctccctgc tcatccttga
    17641 caacttttcc tctggccttg cggccaatcg ttcatcctga actattgctt cctgctcaca
    17701 ccaccccgat gtgatacttc atcctgaagt gtccctggcc ttcctgaccc accgaatcat
    17761 cctgaccggt tctcattctc catcctggag gtgtccttta acgcgtcctg cgtcatcctc
    17821 ttcgcttcat ccagaagcct ttccctgaaa caccatcctg gtgtgtcctg cagaagtgtc
    17881 atcatcctga tgttcacttc gttgtctgac tccctgtcga cgaagataga atcgtctttt
    17941 tcgcgaagtc ttacaaggcg cttacagaca atgccttaag gaaaatttca tatgaaagtt
    18001 aagaattaac aatcaacatt ttgattttaa atgaaaataa cgattcttat ctttttaagt
    18061 attcgcttat ttcctggcga tctcctacag agtctgtaag agatctctca caaccagatg
    18121 gggtatttgg attacagaag aggcttaagt tgattaagga attccgcaag atttgaggct
    18181 aaatgcgtcc gtttacgtgt tcccagcgtc tctttgcaca cttcaccgga gagattacac
    18241 accgagatga cctctaactc ttcttccagc gtcgcgataa atagcgtggg cggcagtttt
    18301 agccttttct gagtcaccag atgaccaatc agattttcct gcacccgacg gaagtcgtct
    18361 tcactccagg tttgcagcaa tgtcagtttg atatcgccaa actgcgcgtg catatccccg
    18421 gcaaactgag tcgtataaaa ggtatgaatt gtgggttgta tcacaatatc aaaagcgcgt
    18481 tcaaccgcgt ttacattttg ttccccggta aacggctggg gttgccagta tacggcatct
    18541 tcggtagttg aaataatgca cggtgaagga acaccgtaca gttcctcact taacggccag
    18601 cttttatgct cttcatgcca cgcatcgcag tagcgtgcag taaaatcttt cagggcttgt
    18661 gcggtcaaat cgtccactaa tttctctctt cacttaaacc agatacactt gtcgtttagt
    18721 ttatctggtt tatgacggtg aaacatgtct tcttatgcaa accatcaggc acttgcgggc
    18781 ctgactcttg gaaaatcaac cgattaccgg gatacctatg acgccaacct actgcaaggc
    18841 gttccacgca gcctgaatcg cgacccgctg ggtctgaaag cggataacct gccttttcac
    18901 ggtacggata tctggacgct gtatgaactt tcctggctga atgcgaaagg tttgccgcag
    18961 gttgccgtcg gtcacgttga acttgattac accagcgcaa atctgattga gtcgaagagt
    19021 tttaagctct atctcaacag ttttaaccag acgcgtttta ataactggga tgaggtgcgc
    19081 cagacgctgg agcgcgactt aagcacttgc gcccagggta aggttagcgt ggcgttatat
    19141 cgtcttgatg aactggaagg ccagccgata ggtcatttca atggcacctg cattgatgac
    19201 caggatatca ctatcgataa ctatgaattc actactgact atctggagaa tgccaccagc
    19261 ggtgaaaaag tagtggaaga gacgcttgtc agccacctgc tgaaatcaaa ctgcctgatc
    19321 acccatcaac cagattgggg ttcgatccaa attcagtatc gtggacgcca aattgacaga
    19381 gaaaaactgc tgcgttacct ggtctcattc cgtcatcaca acgagttcca cgaacagtgc
    19441 gtggaacgca tctttaatga cctgttacgc ttctgccagc cagagaaatt aagcgtttac
    19501 gcacgttata cccgtcgtgg cggtctggac attaacccgt ggcgcagtaa tagcgatttt
    19561 gtcccatcga ccacaagact ggttcggcaa taaatttttt ctcaattttg cgtgctggat
    19621 tcacgcagaa ggttgtgaaa ggtcatcagg cagggctatt gtaatcaaag ggaatgacga
    19681 tattcgtccc ataaggagtt tttcttgatt acacatatta gcccgcttgg ctccatggat
    19741 atgttgtcgc agctggaagt ggatatgctt aaacgcaccg ccagcagcga cctctatcaa
    19801 ctgtttcgca actgttcact tgccgtactg aactccggta gtttgaccga taacagcaaa
    19861 gaattgctgt ctcgttttga aaatttcgat attaacgtcc tgcgccgtga acgcggcgta
    19921 aagctggaac tgattaatcc cccggaagag gcttttgtcg atgggcgaat tattcgcgct
    19981 ttgcaggcca acttgttcgc ggttctgcga gacattctct tcgtttacgg gcaaatccat
    20041 aataccgttc gttttcccaa cctgaatctc gacaactccg tccacatcac taacctggtc
    20101 ttttccatct tgcgtaacgc tcgcgcgctg catgtgggtg aagcgccaaa tatggtggtc
    20161 tgctggggcg gtcactcaat taacgaaaac gagtatttgt atgcccgtcg cgtcggaaac
    20221 cagctgggcc tgcgtgagct gaatatctgc accggctgtg gtccgggagc gatggaagcg
    20281 ccgatgaaag gtgctgcggt cggacacgcg cagcagcgtt acaaagacag tcgttttatt
    20341 ggtatgacag agccgtcgat tatcgccgct gaaccgccta acccgctggt caacgaattg
    20401 atcatcatgc cagatatcga aaaacgtctg gaagcgtttg tccgtatcgc tcacggtatc
    20461 attatcttcc ctggcggtgt gggtacggca gaagagttgc tgtatttgct gggaatttta
    20521 atgaacccgg ccaacaaaga tcaggtttta ccattgatcc tcaccggccc gaaagagagc
    20581 gccgactact tccgcgtact ggacgagttt gtcgtacata cgctgggtga aaacgcgcgc
    20641 cgccattacc gcatcatcat tgatgacgcc gctgaagtcg ctcgtcagat gaaaaaatcg
    20701 atgccgctgg tgaaagaaaa tcgccgtgat acaggcgatg cctacagctt taactggtca
    20761 atgcgcattg cgccagattt gcaaatgccg tttgagccgt ctcacgagaa tatggctaat
    20821 ctgaagcttt acccggatca acctgttgaa gtgctggctg ccgacctgcg ccgtgcgttc
    20881 tccggtattg tggcgggtaa cgtaaaagaa gtcggtattc gcgccattga agagtttggt
    20941 ccttacaaaa tcaacggcga taaagagatt atgcgtcgta tggacgacct gctacagggt
    21001 tttgttgccc agcatcgtat gaagttgcca ggctcagcct acatcccttg ctacgaaatc
    21061 tgcacgtaat ctcccctccc ccggtgacgt ttgttgccgg gttttctcgt ttttggtcac
    21121 ttactcatca actcatttca tttgttatat gaatgtttct taccaccctc acgcgacaaa
    21181 tatcatcaca gttaatatgt catacaattt atattgcaac gcaaacgttt ccctattttc
    21241 ataaaccgta ttttatcgct atgaaaaaga aattatcgcc atgattaact aaaagtattg
    21301 attttttcag ttcaacctac atatattgcg cgccccggaa gaagtcagat gtcgtttaat
    21361 gggcaaatat tgcccttaaa ttctctttta cttttgattt acagagtaaa gcgttgggat
    21421 aatctatctt ccaagtagat tattgtattt gagatcaaga tcactgatag atacataact
    21481 tgtgtgtatc tttccgccct caaattatta cggcggtaaa tgattaagcc atcgccgata
    21541 gacagatttc atttttacgg tcaggcacct tcccgggctg aactggctaa aagctgaatt
    21601 atttgcattc ctccaggaga aatagatgga aacgactcaa accagcacga ttgcgtcgaa
    21661 agactctcgt agtgcctggc gcaagacaga caccatgtgg atgctgggcc tttacggcac
    21721 ggcaatcggc gcgggcgtgc tgttcctgcc aatcaacgcc ggtgttggcg gtatgatccc
    21781 gctgatcatc atggctatcc ttgcgttccc gatgacgttt tttgctcacc gcggcctgac
    21841 tcgcttcgta ctgtctggta aaaacccggg cgaagacatc accgaggttg tagaagaaca
    21901 ctttggtatt ggcgcaggta aactgattac cctgctctac ttcttcgcta tctacccgat
    21961 cctgctggtt tatagcgtgg caatcaccaa taccgttgaa agcttcatgt ctcaccagct
    22021 gggtatgacg ccaccgccgc gtgcgattct gtcgctgatc ctgatcgtgg gtatgatgac
    22081 catcgttcgc ttcggtgagc agatgatcgt taaagcgatg agtattctgg tattcccgtt
    22141 tgttggcgta ctgatgctgc tggctctgta cctcatcccg cagtggaacg gcgcagcact
    22201 ggaaacgctg tctctggaca ctgcatctgc aaccggaaac ggtctgtgga tgaccctgtg
    22261 gctggcaatt ccggtaatgg tgttctcgtt caaccactct ccgatcatct cttctttcgc
    22321 cgttgcgaag cgtgaagagt acggcgatat ggcagaacag aaatgctcga agatccttgc
    22381 attcgcacac atcatgatgg tgctgaccgt aatgttcttc gtcttcagct gcgtactgag
    22441 cctgactccg gcagacctgg ctgcggctaa agagcagaac atctcgattc tgtcttacct
    22501 ggctaaccac tttaacgcac cggttatcgc gtggatggct ccgattatcg cgattatcgc
    22561 tatcaccaaa tccttcctcg gccactacct gggcgcacgt gaaggcttca acggtatggt
    22621 gattaaatct ctgcgtggta aaggtaagtc tatcgaaatc aacaagctga accgtatcac
    22681 tgcgctgttc atgctggtaa cgacctggat tgttgccacc ctgaacccga gcatcctggg
    22741 tatgattgaa accctgggcg gcccaatcat cgcgatgatc ctgttcctga tgccgatgta
    22801 cgcaattcag aaagtaccgg caatgcgtaa gtacagcggt cacatcagca acgtattcgt
    22861 tgtcgtgatg ggtctgattg caatctccgc aatcttctac tctctgttca gctaagtcct
    22921 ttcgcgccgc tttcgggcgg cgcttcctcc gttttaacgc gatgtatttc ctatgattag
    22981 cgtattcgat attttcaaaa tcggcattgg cccttccagt tctcataccg ttggaccaat
    23041 gaaagcgggt aaacaattta ccgacgatct gattgcccgt aatctgctta aagacgtgac
    23101 ccgcgtggtg gttgacgtgt acggctcgct ctctctgacc ggtaaaggcc accacactga
    23161 tatcgccatt attatgggcc tggcgggtaa cctgccggat accgtggata tcgattccat
    23221 ccctggtttt attcaggatg tgaatactca tggtcgcctg atgctggcaa acggtcagca
    23281 tgaagtggag ttcccggttg atcagtgcat gaacttccat gcagacaacc tttctctgca
    23341 tgaaaacggt atgcgcatta ccgcgctggc gggcgataaa gtcgtttaca gccagactta
    23401 ctactctatt ggcggtggct ttatcgttga tgaagagcat tttggccagc agaatagcgc
    23461 accggttgaa gttccttatc cgtacagttc agcagccgat ctgcaaaaac attgtcagga
    23521 aaccgggctg tcactctctg gcctgatgat gaaaaatgag ctggcgctgc acagcaaaga
    23581 agagctggaa cagcacctgg cgaacgtatg ggaagtcatg cgtggcggta ttgagcgcgg
    23641 tatttccacc gaaggcgtgt tgcctggcaa actgcgcgtt ccacgccgtg ctgcggcact
    23701 acgccggatg ctggtcagcc aggataaaac caccactgac ccgatggcgg ttgttgactg
    23761 gatcaacatg tttgcattgg cagtgaacga agagaacgct gctggcggac gcgtggtgac
    23821 tgcgccgact aacggtgcgt gcgggattat cccggcagtg ctggcgtact acgacaagtt
    23881 tatccgcgaa gtgaacgcta actcactggc tcgttacctg ctggtagcca gtgccattgg
    23941 ttctctttat aagatgaacg cgtcaatttc tggtgctgaa gtcggctgcc agggtgaagt
    24001 tggtgtggcg tgctcaatgg cggcggctgg tctggcagaa ctgttaggtg caagcccggc
    24061 gcaggtgtgc atcgcggcgg aaatcgccat ggagcacaac ctcggtctga cgtgtgaccc
    24121 ggtcgccgga caggtccagg tgccatgcat cgagcgtaac gccattgcgg cagtaaaagc
    24181 ggtgaacgcc gcacgtatgg cgctacgccg taccagcgag ccgcgcgtct gcctcgataa
    24241 agttatcgaa accatgtacg aaacaggtaa agatatgaac gccaagtacc gcgaaacctc
    24301 tcgcggcggc ctggcaatga agatcgttgc ctgcgattaa tcgctctcca gaggcctcgt
    24361 tttgcgaggc ctcttcccga tttctcatcc agccgtagcc tgttccggca tcgaatgtta
    24421 cccttatcgc ctgatcttta agggggttat cgtggctgtt catttgctta ttgtcgatgc
    24481 actgaatctt attcgtcgca ttcatgccgt tcagggatcg ccctgtgtcg aaacctgcca
    24541 gcatgcgctc gatcagctca ttatgcacag ccagccaacc cacgcggtcg ccgtttttga
    24601 tgatgaaaac cgcagtagcg gctggcgtca tcagcgttta ccagattaca aagcgggtcg
    24661 accgccaatg ccggaagagt tgcacgacga gatgcctgca ttacgcgccg cctttgagca
    24721 acgcggcgtc ccgtgctggt caaccagcgg caacgaagcc gatgacttag ccgccacgct
    24781 ggcggtcaaa gtgacacagg ccgggcatca ggcaacgatt gtttcgacag ataaaggcta
    24841 ctgtcagtta ctttcaccga cattacgtat tcgtgattac ttccagaaac gttggctgga
    24901 tgcgccattt atcgataaag aatttggcgt tcaaccgcag cagttgcccg attactgggg
    24961 acttgcgggg atcagcagtt caaaggtacc gggtgttgcg ggaatcggac caaaaagcgc
    25021 cacgcagctg ctggtcgagt ttcagagtct ggaaggaata tatgagaatc tggatgcggt
    25081 tgccgaaaag tggcgcaaaa aattagaaac ccataaagag atggcgtttc tgtgccgcga
    25141 tattgcccgc ttacaaaccg atttgcatat cgacggcaat ttacagcaat tgcggttggt
    25201 acggtaacgg cgagccggat acgccgcaaa cgtcgtatcc ggcattatca catcagcgca
    25261 tttaccaggc ggtatggtaa agctctacaa tatcctcaag cgttgcttca cgcgggttgc
    25321 caccggtaca aacatcatcc agtgccgcct gcgccagtgc cggaatgtct tccttgcgta
    25381 caccaacatc acgcaaatgt ggcggaatac cgacatcacg gttgagagca aacaccgctt
    25441 caacagcggc attacgcgcc tcttccaggc tcataccttc cactttcacg cccataacgc
    25501 gcgcgatatc gcggtacttc tcaccggtaa agtcagcgtt atagcgcatg acatgcggta
    25561 acaggatggc gttcgcaaca ccgtgtggag tgttgtaaaa cgcgcccagt ggatgcgcca
    25621 taccatgcac caaccctaac ccaacattcg agaagcccat acccgcaaca tactgcccga
    25681 gcgccatttc ttctccggca tccttatcac cagcaaccga tcctcgcagc gccccagcaa
    25741 tgatttcaat cgctttaatg tgcagtgcat cggttagcgc ccacgcgcca cgggtaatat
    25801 acccctcaat agcatgagtg agcgcatcga cacccgtcgc agctttcagc gctggaggca
    25861 taccatccat catgtcagcg tcaataaacg ccacctgcgg gatatcatgc ggatcaacgc
    25921 aaacaaactt gcgccgtttc tcttcgtcag tgatcacgta gttaatggtc acttctgccg
    25981 cagtacctgc tgtggtagga attgccagaa tcggtacact gggtttattg gtcggggaaa
    26041 gcccttccag gctacgcaca tcggcaaact ccgggttgtt gctgataatg ccaatcgctt
    26101 tacaagtatc ctgtggagaa ccaccaccaa tagcgatcag gtaatccgcg ccgctattct
    26161 ggaatacacc gagcccttct ttgacgacag taattgttgg gttgggcact acgccgtcgt
    26221 aaatcgccca tgccagccct gcagcatcca tcttatcggt cactttcgcc accacgccgc
    26281 attgcaccag cgttttatcg gtgacgatca gcgccttctg ataaccacgg cgtttcacct
    26341 catcggttaa agccccaaca gcaccccgac caaaccatgc cgtttcgttc agaatcattc
    26401 tgttagccat catccttctc cttgttgctt tacgaaatta ctcttcaatc cgtaacccat
    26461 aggttttgaa tttctccagc actacggcaa tctcttcatc gctcagcact ggcaccgggt
    26521 ccgtaatcgc cagggtcgtc aggtaaagtt gcgccagcac ttcaacttca tgcgccagcc
    26581 ataacgcttt ttccagattc acctcacaag cgataagccc atgatgttgt aacaaagttg
    26641 ccttacgatt tttgagagcc tgcgcaacat gttcagaaag ttcacgtgtt ccaaaggtcg
    26701 cataaggcgc gcaaggaata gaattaccgc cagccgccgc aatcatgtag tgaatagcgg
    26761 ggatcgatcg gttaagaatg gaaactgccg tgcaatgaac ggcatgattg tgaacaaccg
    26821 cgttggcatc cggtctgctt tgataggccg ccatatggaa acgccattcg cttgagggga
    26881 gctttccttc ctcatgttta ccgttgccat caataaagac aatatgcgac tccgtcagtt
    26941 tttcatatgg aatgcctgta ggcgtaatca gcatcccatc ctgataacgt acactgacgt
    27001 tccccgctgt cccctggttc agtcccaggc gggtcatttc caggcaagtg tcaataatct
    27061 gacgagcaag tttatttcgt tccattagct acctctctct gattcaaaac agggcaataa
    27121 tgttgttcct ttcacactat tgaattagcc gtttaattac ccaccatctt cttcctgatt
    27181 aaccagaaag aaattcacaa gcttatattt tgtgacctgg ttcaactaat cacagtaaat
    27241 aactgcaagt tctcttttta taaccccatt aaaaatgacc gctcttaaaa atatttatca
    27301 aaacggtcat ttttctattc actcaagcca ggaatgcccg ttttcggcat aaacaattaa
    27361 tacggtcatc tggtttgtgt tttttatgat ttattttctg aaacgggcat gaaatttcga
    27421 ttattaaagt gatggtagtc acataaagtc accttccagc taataagtgt gaccgccgtc
    27481 atattacaga gcgtttttta tttgaaaatg aatccatgag ttcatttcag acaggcaaat
    27541 attcactgat atgaagcccg aactcgctgg ttttgcactt ttgaaaacat aaccgattac
    27601 gtgcttaagc ttctgaacct aagaggatgc tatgggaaac acatcaatac aaacgcagag
    27661 ttaccgtgcg gtagataaag atgcagggca aagcagaagt tacattattc cattcgcgct
    27721 gctgtgctca ctgttttttc tttgggcggt agccaataac cttaacgaca ttttattacc
    27781 tcaattccag caggctttta cgctgacaaa tttccaggct ggcctgatcc aatcggcctt
    27841 ttactttggt tatttcatta tcccaatccc tgctgggata ttgatgaaaa aactcagtta
    27901 taaagcaggg attattaccg gattattttt atatgccttg ggcgctgcat tattctggcc
    27961 cgctgcagaa ataatgaact acaccttatt tttagttggc ctatttatta ttgcagccgg
    28021 attaggttgt ctggaaactg ccgcaaaccc ttttgttacg gtattagggc cggaaagcag
    28081 tggtcacttc cgcttaaatc ttgcgcaaac atttaactcg tttggcgcaa ttatcgcggt
    28141 tgtctttggg caaagtctta ttttgtctaa cgtgccacat caatcgcaag acgttctcga
    28201 taaaatgtct ccagagcaat tgagtgcgta taaacacagc ctggtattat cggtacagac
    28261 accttatatg atcatcgtgg ctatcgtgtt actggtcgcc ctgctgatca tgctgacgaa
    28321 attcccggca ttgcagagtg ataatcacag tgacgccaaa caaggatcgt tctccgcatc
    28381 gctttctcgc ctggcgcgta ttcgccactg gcgctgggca gtattagcgc aattctgcta
    28441 cgttggcgca caaacggcct gctggagcta tttgattcgc tacgctgtag aagaaattcc
    28501 aggtatgact gcaggctttg ccgctaacta tttaaccgga accatggtgt gcttctttat
    28561 tggtcgtttc accggtacct ggctcatcag tcgcttcgca ccacacaaag tcctggccgc
    28621 ctacgcatta atcgctatgg cactgtgcct gatctcagcc ttcgctggcg gtcatgtggg
    28681 cttaatagcc ctgactttat gcagcgcctt tatgtcgatt cagtacccaa caatcttctc
    28741 gctgggcatt aagaatctcg gccaggacac taaatacggt tcgtccttca tcgttatgac
    28801 catcattggc ggcggtattg tcactccggt catgggtttt gtcagtgacg cggcgggcaa
    28861 catccccact gctgaactga tccccgcact ctgcttcgcg gtcatcttta tctttgcccg
    28921 tttccgctct caaacggcaa ctaactgaac atattttccg aataaagtga ggaatctgta
    28981 atgaaaaaaa tcagcttacc gaaaattggt atccgcccgg ttattgacgg tcgtcgcatg
    29041 ggtgttcgtg agtcgcttga agaacaaaca atgaatatgg cgaaagctac ggccgcactg
    29101 ctgaccgaga aactgcgcca tgcctgcgga gctgccgtcg agtgtgtcat ttccgatacc
    29161 tgtatcgcgg gtatggctga agccgctgct tgcgaagaaa aattcagcag tcagaatgta
    29221 ggcctcacca ttacggtaac gccttgctgg tgctatggca gtgaaaccat cgacatggat
    29281 ccaacccgcc cgaaagccat ttggggcttt aacggcactg aacgtcccgg cgctgtttac
    29341 ctggcagcgg ctctggcagc tcacagccag aaaggcatcc cggcattctc catttacggt
    29401 catgacgttc aggatgccga tgacacatcg attcctgccg atgttgaaga aaaactgctg
    29461 cgctttgccc gcgccggttt ggccgtcgcc agcatgaaag gtaaaagcta tctgtcgctg
    29521 ggcggcgttt cgatgggtat cgccggttcc attgttgatc acaacttctt tgaatcctgg
    29581 ctgggaatga aagttcaggc ggtggatatg accgaactgc gtcgccgtat cgatcagaag
    29641 atttacgacg aagccgaact ggaaatggcg ctggcctggg ctgataaaaa cttccgctat
    29701 ggcgaagatg aaaataacaa acagtatcaa cgcaatgccg agcaaagccg cgcagttctg
    29761 cgcgaaagtt tactgatggc gatgtgtatc cgcgacatga tgcaaggcaa cagcaaactg
    29821 gccgatattg gtcgcgtgga agaatcactt ggctacaacg ccatcgctgc gggcttccag
    29881 gggcaacgtc actggaccga tcaatatccc aatggtgaca ccgccgaagc gatcctcaac
    29941 agttcatttg actggaatgg cgtgcgcgaa ccctttgtcg tggcgaccga aaacgacagt
    30001 cttaacggcg tggcaatgct aatgggtcac cagctcaccg gcaccgctca ggtatttgcc
    30061 gatgtgcgta cctactggtc accagaagca attgagcgtg taacggggca taaactcgat
    30121 ggactggcag aacacggcat catccatttg atcaactcag gttctgctgc gctggacggt
    30181 tcctgtaaac aacgcgacag cgaaggtaac ccgacgatga agccgcactg ggaaatctct
    30241 cagcaagagg ctgacgcttg cctcgccgct accgaatggt gcccggcgat ccacgaatac
    30301 ttccgtggcg gcggttactc ttcccgcttc cttaccgaag gcggcgtccc gttcaccatg
    30361 actcgtgtca acatcatcaa aggcctggga ccggtactgc aaatcgcgga aggctggagc
    30421 gtggaattgc cgaaggatgt gcatgacatc ctcaacaaac gcaccaactc aacctggcca
    30481 accacctggt ttgcaccgcg cctcactggt aaagggccgt ttacggatgt gtattcggta
    30541 atggcgaact ggggtgccaa ccacggagtt ctgaccatcg gccacgttgg cgcagacttt
    30601 atcactcttg cctccatgct gcgtatcccg gtatgtatgc acaacgttga agagaccaaa
    30661 gtgtatcgtc cttctgcctg ggctgcgcac ggcatggata ttgaaggcca ggattaccgc
    30721 gcttgccaga actacggtcc gttgtacaag cgttaataca ttactccctg atgtgatgcc
    30781 cggtcgctcc ggctaccggg cctgaacaag caagagtggt tagccggata aacaatgtta
    30841 tccggctata ttgcaggagc gattatgaaa caagaagtta tcctggtact cgactgtggc
    30901 gcgaccaatg tcagggccat cgcggttaat cggcagggaa aaattgttgc ccgcgcctca
    30961 acgcctaatg ccagcgatat cgcgatggaa aacaacacct ggcaccagtg gtctttagac
    31021 gccattttgc aacgctttgc tgattgctgt cggcaaatca atagtgaact gactgaatgc
    31081 cacatccgcg gtatcgccgt caccaccttt ggtgtggatg gcgctctggt agataagcaa
    31141 ggcaatctgc tctatccgat cattagctgg aaatgtccgc gaacagcagc ggttatggac
    31201 aatattgaac ggttaatctc cgcacagcgg ttgcaggcta tttctggcgt cggagccttt
    31261 agtttcaata cgttatataa gttggtgtgg ttgaaagaaa atcatccaca actgctggaa
    31321 cgcgcgcacg cctggctctt tatttcgtcg ctgattaacc accgtttaac cggcgaattc
    31381 actactgata tcacgatggc cggaaccagc cagatgctgg atatccagca acgcgatttc
    31441 agtccgcaaa ttttacaagc caccggtatt ccacgccgac tcttccctcg tctggtggaa
    31501 gcgggtgaac agattggtac gctacagaac agcgccgcag caatgctcgg cttacccgtt
    31561 ggcataccgg tgatttccgc aggtcacgat acccagttcg ccctttttgg cgctggtgcc
    31621 gaccaaaatg aacccgtgct ctcttccggt acatgggaaa ttttaatggt ccgtagcgcc
    31681 caggttgata cttcgctgtt aagtcagtac gccggttcca cctgcgaact ggatagccag
    31741 gcagggttgt ataacccagg tatgcaatgg ctggcatccg gcgtgctgga atgggtgaga
    31801 aaactgttct ggacggctga aacaccctgg caaatgttga ttgaagaagc tcgtctgatc
    31861 gcgcctggcg cggatggcgt aaaaatgcag tgtgatttat tgtcgtgtca gaacgctggc
    31921 tggcaaggag tgacgcttaa taccacgcgg gggcatttct atcgcgcggc gctggaaggg
    31981 ttaactgcgc aattacagcg caatctacag atgctggaaa aaatcgggca ctttaaggcc
    32041 tctgaattat tgttagtcgg tggaggaagt cgcaacacat tgtggaatca gattaaagcc
    32101 aatatgcttg atattccggt aaaagttctc gacgacgccg aaacgaccgt cgcaggagct
    32161 gcgctgttcg gttggtatgg cgtaggggaa tttaacagcc cggaagaagc ccgcgcacag
    32221 attcattatc agtaccgtta tttctacccg caaactgaac ctgaatttat agaggaagtg
    32281 tgaaatgctg aaaacaattt cgccgttaat ttctcccgaa ctattgaaag tgctggcaga
    32341 gatgggacat ggagatgaaa ttattttttc cgatgctcac tttcccgccc attcgatggg
    32401 accgcaggtg atccgcgctg atggcctgtt ggtgagcgac ttgctccagg cgattatccc
    32461 gttatttgaa ctggacagtt atgcaccgcc gctggtgatg atggcggcgg tagaaggtga
    32521 cactctcgat cctgaagtag aacgacgtta ccgtaatgcg ctttcactac aagccccgtg
    32581 tcctgacatc atccgcatca atcgttttgc gttttatgaa cgggcgcaaa aagcctttgc
    32641 gatcgttatc acaggcgaac gagcgaagta cgggaatatt cttttaaaaa aaggggtaac
    32701 accgtaatct cataccggta cgcccgcatg acgcgggcgg ttatcgaatg atggggtgaa
    32761 aaatatgaaa gcggcacgcc agcaagcgat agtcgacctg ctgctgaacc ataccagcct
    32821 gaccacggaa gctctctctg aacagctaaa ggtcagtaaa gaaaccattc gtcgcgatct
    32881 caatgaatta cagacgcagg gtaaaattct gcgcaatcat ggacgcgcta aatatatcca
    32941 ccgtcaaaat caagacagtg gcgatccctt tcacatcagg ctgaaaagcc attatgcgca
    33001 taaagcagat atcgcgcgcg aggcgctcgc gtggattgaa gaagggatgg tgatagcctt
    33061 agacgccagt tcaacttgct ggtatctggc acgccagttg cctgacatca acattcaggt
    33121 cttcaccaat agccatccga tttgccatga actcggtaaa cgcgaacgca ttcaactgat
    33181 cagttccggc ggcacacttg agcgcaaata tggctgttac gtcaatccct cgctgatttc
    33241 ccaacttaaa tcgctggaaa tcgatctgtt tattttttct tgtgaaggga tcgatagcag
    33301 cggcgcactg tgggactcca atgcgatcaa cgctgattac aaatcgatgc tattaaaacg
    33361 tgccgcgcaa tcgttgttat tgattgataa aagtaaattt aatcgttcag gggaagcccg
    33421 catcgggcat ctggatgagg taacgcacat tatttctgat gagcgccagg ttgcaacttc
    33481 tttggtaaca gcctgacgga aagggtagca ggccggagac gacgccccgg ccttgcctgt
    33541 tatcgctcgt cgcgacgacc acccaccgca gcccagatgc ggcggacgtg caccgtcact
    33601 tcttcgcgat cgtgatacaa ctgccgcgcc tgaatctgag catttatgcc atgttcatca
    33661 agctgtgcct gaatatacgc cagattgtgt gacacttctt cgtagcgttt tttcatcggc
    33721 agtttgaggt tgaaaatggt ttcacggcac cagccattaa ccagccactg cgccatcaat
    33781 gccgcaactt tcgccggttt ttcaaccata tcgcatacca tccaggagat attgctgcgc
    33841 gtcggacgga atttgaaacc gtcttcccgc agccacgtca cctgtccggt atccatcaga
    33901 ctttgcgcca tcgggccgtt gtcgacggaa taaacccaca tgttgcgctt caccagttgg
    33961 taggtccagc cgccagggca agcgcctaaa tccaccgccc acatcccgtt cgccaggcgt
    34021 tcatcccact catccgcagg aataaacaca tgaaatgcct cttccagttt gagcgtggaa
    34081 cgactcggcg catctgccgg aaatttcagg cgcggaatgc ccatatagaa cggcgaatta
    34141 ttgttgctgt atgagtaacc ggtatagcag cagcctggtg caataaagaa tacatgcaca
    34201 accggacgct tcggcgtttc atagttcgcc agcaccccgg catcgcgcaa ggcagcgcgt
    34261 agcggaacag taaatttacg gcagaatttc agtaactctt tgctttcgtt ggtatcggca
    34321 acttcaacac gcagttcacc gcccttctct actacgccct gtaacatgcc gacaatgggg
    34381 gtaatacgat cttctggcgg caaatgctgc aggagttccc ccaccacaaa ccactggcgg
    34441 gcaaaaatta atgaactgaa cggcagctca cggattaact tatcgccatc atcaggttga
    34501 taacattcat aaatgacata acccgcattc tctttcacgc gggcaaaacc gaaaatttcc
    34561 cgctggccgg ctttatcggt aatttctgcg gcgcactctt tttcaaagcc cggacggcac
    34621 agcaatacaa ccttattcat gacttacgcc cttgcgcttt aaacggatag caccaactaa
    34681 cattaacgcc cagcccgcga ggaagctcac gccgccaacc ggagtgacaa acgcccacaa
    34741 acgcagatgg gacagcgcca ggcaataaag gctgccgctg aacaacaccg tgcctaacgc
    34801 gaggaaaacg ctactccagt aaaaccagat actgatgcga cgctgcattg ccaccgccag
    34861 acctaagatc gccagcgtat gaaacgcctg gtattcgagg ccggtctgga tccagcccat
    34921 ctcaacggcc cccatggttt tacttaacac atgcgcgcca aaagcgccca gagccacaaa
    34981 aatgaagccg ctaatggcgg cgaaaatcag cataaaacgg ctggtcatgg tcgtacccta
    35041 cgtaaattat tgttcataac gaaagcggaa tttttcttgt tcagcagcgg ctttcgccag
    35101 gatccattgg cgaaaggcgg ctattttacc cagttctgcc tggctgtcat gacaaaccag
    35161 ataaaaagcg tttttactga ccagaacatc attaaacggg caaacaagac gtccggcctc
    35221 gatttcagat tgcgccatca cgttatttgc cagcgccact ccctgcccgt gaatagccgc
    35281 ttgcagcacc atggcgctat gactaaaaat tggcccttgc tgaacgttga tatgatttaa
    35341 ccccaactgt cgggtatatg tctgccagtc acggcgtgac gcatcatgta ataacgtatg
    35401 tttagccaga tcttccgggg tcttcaaggg tttttcgcct gtcagcagta gcggcgaaca
    35461 caccggcaat aaatattcgg cgtacagttt ttccacccgt agccccggcc agttgccccg
    35521 accataaaat atcgccacat caacatcatc cgccagctta tcttcctgac gatcaaccgc
    35581 ctggattcga acgtcaattc ccggataagc tgaattaaag ctggaaagtc gcggaaccaa
    35641 ccaatgaatg gcgaaactgg ggagtaaact gaccgtcaac gcccccttgg cgctacgggc
    35701 ctggagttta cgcgtcgctt cggttaattg cgaaaatatc tctttgatat cgaggaaata
    35761 gctttgccct tcctcggtca gcaggagtga acgattacgg cggcggaaca gttttagccc
    35821 caaaaaatcc tcaagagact tgatttgatg acttactgcg gcctgggtca caaaaagctc
    35881 ttctgctgcg cgagtgaaac ttaaatggcg tgctgcggca tcaaaaactc gtaaggcatt
    35941 tagcggtggt aatcgtttag acatggcttt taaactttga tgttaaatga atttaacaat
    36001 tagatcacac tgtgtaacct attagttttt ttaatctgag ccattataaa ttgtccgttg
    36061 agcttctacc agcaaatacc tatagtggcg gcacttcctg agccggaacg aaaagtttta
    36121 tcggaatgcg tgttctggtg aacttttggc ttacggttgt gatgttgtgt tgttgtgttt
    36181 gcaattggtc tgcgattcag accatggtag caaagctacc ttttttcact tcctgtacat
    36241 ttaccctgtc tgtccatagt gattaatgta gcaccgccta attgcggtgc ttttttttac
    36301 cttgcgatcg cgaattactg atccagttcg accatctctt tcacgtcagt acggttgatc
    36361 tgctgtttgt tgccattagc gtctttatac gaaatcatac cggtatcgtt atcagtctgt
    36421 ggtttgccgt cagagacgat ggtacgtccg tcattggtgt gcatcacata gttcgaaccg
    36481 gaacaggcgc tcagggcaaa agtcagcata caggcagaaa taattgcggc agtctttttc
    36541 atcatcttct ccttaaagcg attaataaat cttaatagta ttgattaaca ggctaaaatt
    36601 aacgcctaac actattcagc atatgttact tacgcggctt cgccaggata tccagataat
    36661 tctgatggtt agcactctcc ttgtatcaaa gtgaattttg cgtcacgatc ggtgcatcaa
    36721 gccgaggagt accatgaacg tttttaatcc cgcgcagttt cgcgcccagt ttcccgcact
    36781 acaggatgcg ggcgtctatc tcgatagcgc cgcgaccgcg cttaaacctg aagccgtggt
    36841 tgaagccact cgacagtttt acagcctgag cgccggaaac gtccatcgca gccagtttgc
    36901 cgaagcccaa cgcctgaccg cgcgttatga agctgcacga gagaaagtgg cgcaattact
    36961 gaatgcaccg gatgataaaa ctatcgtctg gacgcgcggc accactgaat ccatcaacat
    37021 ggtggcacaa tgctatgcgc gtccgcgtct gcaaccgggc gatgagatta ttgtcagcgt
    37081 ggcagaacac cacgctaacc tcgtcccctg gctgatggtc gcccaacaaa ctggagccaa
    37141 agtggtgaaa ttgccgctta atgcgcagcg actgccggat gtcgatttgt tgccagaact
    37201 gattactccc cgtagtcgga ttctggcgtt gggtcagatg tcgaacgtta ctggcggttg
    37261 cccggatctg gcgcgagcga ttacctttgc tcattcagcc gggatggtgg tgatggttga
    37321 tggtgctcag ggggcagtgc atttccccgc ggatgttcag caactggata ttgatttcta
    37381 tgctttttca ggtcacaaac tgtatggccc gacaggtatc ggcgtgctgt atggtaaatc
    37441 agaactgctg gaggcgatgt cgccctggct gggcggcggc aaaatggttc acgaagtgag
    37501 ttttgacggc ttcacgactc aatctgcgcc gtggaaactg gaagctggaa cgccaaatgt
    37561 cgctggtgtc ataggattaa gcgcggcgct ggaatggctg gcagattacg atatcaacca
    37621 ggccgaaagc tggagccgta gcttagcaac gctggcggaa gatgcgctgg cgaaacgtcc
    37681 cggctttcgt tcattccgct gccaggattc cagcctgctg gcctttgatt ttgctggcgt
    37741 tcatcatagc gatatggtga cgctgctggc ggagtacggt attgccctgc gggccgggca
    37801 gcattgcgct cagccgctac tggcagaatt aggcgtaacc ggcacactgc gcgcctcttt
    37861 tgcgccatat aatacaaaga gtgatgtgga tgcgctggtg aatgccgttg accgcgcgct
    37921 ggaattattg gtggattaat gacaaacccg caattcgccg gacatccgtt cggcacaacc
    37981 gtaaccgcag aaacgttacg caataccttc gcaccgttgt cgcaatggga agataaatat
    38041 cgccagttga tcatgctggg gaaacagctt ccggctttgc cagacgagtt aaaagcgcag
    38101 gctaaagaga ttgccggatg cgaaaaccgc gtctggctgg gatataccgt gactgaaaac
    38161 ggtaaaatgc acttctttgg cgacagcgaa gggcgcattg tgcgcggcct gttggcggtg
    38221 ttgttgactg ccgttgaggg gaaaaccgtc gccgaattgc aggcacagtc gccgctggca
    38281 ttgtttgatg agctgggatt gcgtgcacag cttagcgcct cacgcagcca ggggttgaat
    38341 gcgttaagcg aggcgattat cgctgcggcg aagcaggttt aagcacagaa ccaggtcgga
    38401 taagacgttc gcgtcgcatc cgacctgatt gtttaaccct gacgcgccgc tttcgccatc
    38461 atcttcttca gcgcatgaga aaccgcaaca aacccaaagg tggcggtcac catcgttgcc
    38521 gcgccaaatc cagatgcaca atccatccgc ttcggccctt ctgccgtggc tttcatcgca
    38581 cacacagtac cgtctgactg cgggtacacc agcgcttcag tagaaaacac gcaatcgacg
    38641 ccgagcttac ctttactgtt tttcactacg ccaaaatcgc ttttcaggcg ctcgcgcaac
    38701 ttcgccgcca gcggatcctg aatcgttttc gccagatcgg taacctgaat ctgcgacggg
    38761 tcaatctgcc cacccgcgcc accggtcgtg accagcggga ttttattgcg ccgacaataa
    38821 gcaatcagcg ccgctttggg ccgtacacta tcaatggcat caatcacgta cgaataaccc
    38881 acgctcatat actgcgctac gttatccggc gtcacgaaat catccaccac cgttacacgg
    38941 cactccgggt taatctggcg aatacgctcc gccatgactt ccgcttttgc cagcccgacg
    39001 ttatcgcgca gagcgtgaat ttgccgattg gtattggtga cgcacacatc atccatatcg
    39061 ataagcgtga ttgcgccaat ccccgtgcgc gccagcgctt ccgccgccca ggaaccgaca
    39121 ccaccgatac caaccacaca aatatgcgcg tcagcaaaca gttgcaacgc tttttcacca
    39181 tacagacgcg ctgtgccacc aaaacgctga cgccatgcat cactaattac cacagacata
    39241 gcacctcaga attaaaaagg gtgaggttat ccctcaccct ggcgtatccg taaaaacggt
    39301 atacagaata acacatcagc cgctaaagac gttacctgcg cccggggcgg ttttcagcac
    39361 ccagacacgt ccatagtggt tgtaccaacc tgcgcgatgt ccggcttccg gcccgatccc
    39421 ttgatagata tcgaagtgtt ggcctttgat tgcgccaccg acatccagcg ccaccatcag
    39481 acgcaattcg tactgaccgt taaatttgcc gttattatcc agcagcggca cttctgccag
    39541 caaggtagta cctggcggaa taattgaacg atcggaggca acagaagcac ggccaaccag
    39601 cggcaccgca cttgcccctt tcaccggtgc aaaagattgc ggtttaaaga aaacgaaaga
    39661 cgggttctgt tccagcagct cgcgaacctc ggcttcactg tgtgtttcgc cccaatgacg
    39721 aatcgcctgc atcgacatat cttctttttt cacttcgcca cggtcgatca gcaccttacc
    39781 aatgctgcga taggcatgac cgtttttccc tgcatagctg aaaaagttaa gcggactgcc
    39841 atcaccaaag tcgatatacc cgctaccctg cacatccata atgaagttat ccatcaggga
    39901 gttgctgtaa gcgagaatat atttatcact cagcgcccct gcgtagacct ccgcacgaga
    39961 cggcagacga ccacgttttg gcggcatacg gtaaatagga tactggaact cgccctggcg
    40021 ggtatggcgc gcctgaatta ccggcgtgta atagcctgta aactgcacgt taccatagtt
    40081 gtcggcacct tccatctgcc aggcatcaat gccgaactgg cgcatattgc gggtatcacc
    40141 gcctgcgcgc agccactctt gcaccgcgtt ataaacatta ctctggttgc catacagacg
    40201 cggtgacgaa ttacggatat ggttaatttg ctcggcaaaa tcaccggcgt taatcggcgc
    40261 gccaacggca tctggctggt tcaccagaga gaaaggctgg gtaaatttcc cgtctttata
    40321 ttgctgtccg cgatcggttg gtttggaaga gcaggcggca agcattgcca caaccgtgcc
    40381 cataagaagg tactttaccc aacgtccttt cattgttctc ttcttcaggt taaaaaataa
    40441 ggcgcaacga agataacaaa ccgccgcgtt taatgaaatg agcagcctct ccctgacgca
    40501 aattttgcac aaaaaatagg ctttagtggt ttgtttttgt tcaaaatcat gctaaatccg
    40561 tgatcggggt aaaaaaaagg ttgcatgaaa acgcgagcgg agtatagtgc gcatccacgg
    40621 acgcggggtg gagcagcctg gtagctcgtc gggctcataa cccgaaggtc gtcggttcaa
    40681 atccggcccc cgcaaccaat tattgaacac cctaacgggt gtttttttgt ttctgctctc
    40741 ccataaaaaa agcgccattc agcgcctttt tatcatcccc ttctcgccag cgtcgcccca
    40801 tcggcaaaat acgctttaat tcccgcaaga atagactccg caacttcctg ctggaaagtc
    40861 gccgttttca gtttacgctc ttcctcaacg ttactgataa acgccgtttc gaccagaatg
    40921 gagggaatat caggcgcttt cagcacggca aacccggcct gttcaacctg atttttatgc
    40981 agcttgttga ttttaccgag cttattcagc accgctttac caaacttaag gctgtcggca
    41041 atggtcagcg actgcaccat atcgaacatg gtgtggtcga catagcggtc accgcttttg
    41101 ctcacgccac caatcaagtc cgaggcgttc tgggtctgtg ccagatattt tgccgcagta
    41161 ctggttgcgc ctttggttga gagcgcaaac actgaggaac cgctcggctg gcgactggta
    41221 aaggcgtcgg catggataga gacaaacagg tcggcacgct gtttctgggc ttttgctacg
    41281 cgcacttgca acggaatgaa gatgtcttca ttgcgcgtca tgtacacctt catattgccc
    41341 tctttctcga tcagagagcg cagacggcga gctatttgca ataccacgtc tttttcgcgc
    41401 gttttgtatt tccccaccgc accggagtct tcgccgccgt ggccagggtc aagcataata
    41461 acaataggac gatcgcgccc tgctttaccc ggttgtggac cactttgtgc tggcggcacc
    41521 tgcttttcga ggtcgccttt gttgtaatcc tccagcagcg ccagcagcgg gtcctgcata
    41581 tcctgtgcat tggccggata gaggtccatc accagacgct ctttaaaccc ggcgaccggc
    41641 gcaagggcaa acagctgcgg ttttacgttt tgctttaatt caaaaaccat ccgtacggtc
    41701 tgcgggtcaa attgcccgac gcgcgccgac ttgatgaacg ggtcgtctgc acgaatttgt
    41761 gccgccatcc ccttgagtac cgagttcagg tttacatctt cgatatccac caccacgcgt
    41821 tccgggttac tcaacgcgaa ctgcttatat ttcagctgac gatttgattc taccgtcacg
    41881 cgggtgtagc tggacgcagg ccagacgcgc accgccacga cctggctaac cgcagccagg
    41941 ctgacctgac ttacgctcaa tagccacata gcgcccgcgc cttgcagtaa acgacgacgg
    42001 ctgattgcag tgttggatcc tgacatgcct ctcccgagca aaagaaatct aagctgtgta
    42061 acaagtaaac gactaatttg accggaaact ttagcgaaag acgcataatc tgtcatctaa
    42121 taaacggtaa acattctttt tatattcacg gcattactga taaaaaagtc gctctcgcat
    42181 aaaatttaca cttgcaccct gcgaaaaaac agaataaaaa tacaataatt tcgaataata
    42241 atgcaaaggg gtgtgccgtg gtaaaggaac gtaaaaccga gttggtcgag ggattccgcc
    42301 attcggttcc ctatatcaat acccaccggg gaaaaacgtt tgtcatcatg ctcggcggtg
    42361 aagccattga gcatgagaat ttctccagta tcgttaatga tatcgggttg ttgcacagcc
    42421 tcggcatccg tctggtggtg gtctatggcg cacgtccgca gatcgacgca aatctggctg
    42481 cgcatcacca cgaaccgctg tatcacaaga atatacgtgt gaccgacgcc aaaacactgg
    42541 aactggtgaa gcaggctgcg ggaacattgc aactggatat tactgctcgc ctgtcgatga
    42601 gtctcaataa cacgccgcta cagggcgcgc atatcaacgt cgttagtggc aattttatta
    42661 ttgcccagcc gctgggcgtc gatgacggcg tggattactg ccatagcggg cgtatccggc
    42721 ggattgatga agacgcgatc catcgtcaac tggacagcgg tgcaatagtg ctaatggggc
    42781 cggtcgctgt ttcagtcact ggcgagagct ttaatctgac ctcggaagag attgccactc
    42841 aactagccat caaactgaaa gctgaaaaga tgattggttt ttgctcttcc cagggcgtta
    42901 ctaatgacga cggtgatatt gtctcagaac ttttccctaa cgaagcgcaa gcgcgggtag
    42961 aagcccagga agagaaaggc gattacaact ccggtacggt gcgctttttg cgtggcgcag
    43021 tgaaagcctg ccgcagcggc gtgcgtcgct gtcatttaat cagttatcag gaagatggcg
    43081 cgctgttgca agagttgttc tcacgcgacg gtatcggtac gcagattgtg atggaaagcg
    43141 ccgagcagat tcgtcgcgca acaatcaacg atattggcgg tattctggag ttgattcgcc
    43201 cgctggagca gcaaggtatt ctggtgcgcc gttctcgcga gcagctggag atggaaatcg
    43261 acaaattcac cattattcag cgcgataaca cgactattgc ctgcgccgcg ctctatccgt
    43321 tcccggaaga gaagattggg gaaatggcct gtgtggcagt tcacccggat taccgcagtt
    43381 catcacgggg cgaggttctg ctggaacgca ttaccgctca ggcgaagcag agcggcttaa
    43441 gcaaattgtt tgtgctgacc acgcgcagta ttcactggtt ccaggaacgt ggatttaccc
    43501 cggtggatat tgatttactg cccgagagca aaaagcagtt gtacaactac cagcgtaaat
    43561 ccaaagtgtt gatggcggat ttagggtaag cgatgaaaat tgtcggatgc gacatgcgta
    43621 acactcgtgc gtcgcatccg gcaattacgt ttattcccgc gaactaaaca acgccgccag
    43681 accactgcgc cgctcagtac gagtggcgat tgccgcactt aatatacgct catcggcata
    43741 cagcgacaga cggcgacgcg cgcgggtcac cgcggtgtat accagctctc gcgttactac
    43801 cggcgtgcgt tggctcggta aaatcaacgc cgcatggtcg aactccgatc cctgcgattt
    43861 atgtaccgtc atcgcccacg tagtttcgtg ctctggcaag cgactcggtt gcacagactt
    43921 aatattgccg tccggcatcg caaaccagac gcgcgtcccc tgcccgcgat ccagcgcaat
    43981 accgatatcg ccattaaaca acccaagcgc gctgtcatta cgggcaatca ttaccggtcg
    44041 accttcgtac caacgagagt gcggatgacg atgaattttg cgcttctgtt gcataaactg
    44101 ctcaattcgc tcattcagtc cagccacgcc aaacggccct tcccgcaggg cgcacaaaag
    44161 ttggtactca ttgaacgcct gaatgattaa atccggctcg gcacgcgctt gcagcagatc
    44221 cagataacgt ccataacccg caagagcttc ctcaagcatc gcaatataat cttctccgct
    44281 ctgtaaaagc cgtttttcga tatcagtaaa atcctgctga aaaacggttt tcactgccgt
    44341 tttatcacca cggttgatcg ccgcagctaa ctgaccaatg ccagaatcac tgccgaaacg
    44401 atagcttttt tgcagcaggc agagactgtc gcgcaaagat gccgcttctg tgccagttcc
    44461 tgccggaacg tgagttcccg tcaggcggct tagctgcccg gcacgctcgg cggtaaagcc
    44521 ggcattggca taagcgcaga tatcgcccag cacagcccca gcctcaaccg aggccagttg
    44581 atcacgatcg ccgagaaaga tcactcgcgc atgatcgggc aaggcgtcga tcagtctcga
    44641 catcataggc agatcgatca ttgacgcttc atctaccacc agcacatcaa gatgcagcgg
    44701 gttaccggca tgatgacgta aacgctggct acccggctgc gcacccagca atcggtgcaa
    44761 agtactggca tcttccggaa tgcgtttctt ttgttcatcg gtcagcggta actgtcgcaa
    44821 agccttgccg agagattcgg ttaagcgcgc ggcagcttta cccgttggtg cagccagacg
    44881 gatacggcag cgttcgccgt cggccatttg aattaacgct gccagtaact tcgccacggt
    44941 ggtcgtttta ccggtgcctg ggccgccgga aatcaccgag atccgccgcg tcagcgccac
    45001 tgccgctgca actttttgcc agttaatttc atcgcttact ggaaaaagtt tgtccagggt
    45061 ttgcgccagt agagcttcat caacctcaat ggcatgattc acctcattga agaagcgcgc
    45121 taccgtccgc tcgttacacc acatgcgatt caaataaaga cgatcgccac agaggatcat
    45181 cggcgtgggt tcatctcccc tgctgaccgc ttgagaagcc agcaagcatt cttcccaatt
    45241 ttgtagctca ccgatttcac tgacacaggt cgccaacagc ggatgcgacg cctcgttatt
    45301 ttccagtcgt gaaagcggca aacagacgtg tccctctccg gcatcatgac ttaacagtgc
    45361 cgccgcgagg gtgacggcag gatgttcatc tcccgccacg gtcagggcaa attgcacatc
    45421 cagcgggcgt agctgtttgt gctccacagc ttccagtaat tgcttttgca atttcattac
    45481 gcctcctcca gggtcatacc ggcaaacatc tcatccatca gggcaatcaa cccggcgttg
    45541 ggtcgggttg tgtaaatccc ttgttgcgga tgttctttat caacgccacg caggaacaga
    45601 tagataacgc cgccaaagtg acgatcatag tcgtaatcag caatgcgatg gcgcagataa
    45661 cgatgcagcg ccagggtata aagctgatat tgcagatcat agcggtgtgc ctgcatcgct
    45721 gctgccatag cctgttgggt gtaagccgaa ctgtcttcac ccagccagtt ggatttatag
    45781 tcgagcaggt aataacgccc ttcgtggcgg aataccaggt cgataaagcc ttttaacatg
    45841 ccgcgtacct gcatgaactc cagcggcggg cagccagcgg atagcgggtc aaactggcga
    45901 attagtgcat caagctggct ggcgatgagc ggttcgctaa tcggcaggta aaactccatc
    45961 tccacctgtt tatcgcggtc ggaaagctga ctcaggctaa cacccgtttc attgagaggt
    46021 gcctggagga cagccgtgat ccactcggtc aacaccggtt cccactgcga ttcaaagccg
    46081 ccgagttcca gtttttcctg tacccagttc gggtcaaccg gctgggtaaa atcgaggtct
    46141 tcaaacaaac tgtgcaagaa tgtccccggt gacgcaccgc gcgggaactg atgcggtgtt
    46201 aacgtcggtt cttcaacgac gctgaccacg cctgcggcat caacatccag ccgtggcatc
    46261 agatcctgag cgataccgtg accacgctgc tgcaagccgg agtagctggt gacacgccag
    46321 ttatcgccgg gcaatcgttg tagtgtcctc gcattcagtt ctgcagttaa agcatcatta
    46381 acctgccagg gctggttatc accagtttgt gccgtttgcc aggcaatatc atcatcgcat
    46441 aacgcttcaa tacaggtgcg aagccctgcc gcatcttgcg gttccccttt ttgcagcaaa
    46501 cgcccgagcg cactttggtg gacgtcggtg tcaccttttt tatcgccacg acggcgcacc
    46561 agcggtgcaa cgccgagact gcaatgccaa accgaacgcg tcagcgccac gtaaagcaaa
    46621 cgcagatctt ccgccagacg ttcgacctcc gcgaggtcga cgctttctgg cgcagcgtta
    46681 agatccaaaa ctgcctcaaa cgagtggcga tcgtgataaa acgcctgatc ctggacgcgg
    46741 aaattggtga taaacggcag ccagaccaat ggatattcca gccctttcga tttgtggatc
    46801 gtgacaatct gcaccagatg tttatcactt tcgagacgca tttgttggct ggaggcatta
    46861 ctgtctggct cgaggatatg ttgcgataac cagcgtacca gcgcatgttc actttccagc
    46921 tgcgttccgg cttcttgtag cagttcgctg atatgcaaga tatcggtaag acgccgctca
    46981 ccgcctgccg ttgccagcaa gttttcagca atgttacgcg ccgacatcag cgcccgcagc
    47041 atcggcataa cgccacgttt gcgccagatt tgccgataac catcgaactc ttcgactacc
    47101 acatcccacg catgttcgtc attgttcagc gtttcgatat ccagcgcgtt cagccccatc
    47161 attgacgttg ccagcgcact acgcagggtg ttctcacgtt cgggcgtcat caccgcctgc
    47221 aacaaccaaa gcatttcctg cgcttccaga gtttcaaaaa cactgtcgcg gttcgaaagg
    47281 taaacggaag ggatttccag caacgttaag gcgtcgcgca cctgggcggc ctcctggcgg
    47341 ctgcgcacca gcacactgat gtccgaagca cgcaccggac gcgcgtcgtc gccgttcatc
    47401 agcaacgctt cgccccgctg tccggcttgt agccagtcgc ggatttgcgc agcacatacc
    47461 tgcgccatgg tactttgata gtcgccaacg ccgcagcttt cgccttccat cagccacatt
    47521 ttcatcgcag gctgcgtttc acctttaaac acaaaacgta acgcctgatt tttcccggct
    47581 gatttcactg gaataaacgg tatttcgcga aacatgaacg cgtcatcagt ctggctgaaa
    47641 agcttgttca cactgttcac cattcctggt gcggaacgcc agttggtgtc taaagtgtag
    47701 tgggcgtgaa cttcgctacg cgccttcata taagtgaaga tatccgcacc ccggaatgca
    47761 tatatggcct gtttcgggtc gccaatcagc aacaatgcgg tttccggctg atggtgccag
    47821 atacggcgaa aaattcggta ctgctggggg tcggtatcct gaaattcatc aatcattgcc
    47881 accgggaatc gcgtacggat cgccgctgcc aacacctcgc cgctttcgct acgcagcgcg
    47941 gaatcgagcc gacttaacat gtcatcaaaa cccaattcgc cacggcggcg tttttcacgc
    48001 gctactgttt cgcggatctc agccaatgcg cgggttatca ccagatcacg gatcgacaat
    48061 ggttctgcaa gcagttgttc gatcgcctca aacagtggat gtcgcggggt ttcccccccg
    48121 gctttcgtgc gatcttctaa gaaacgctga gagaattttt ccagcgactc cggtaactga
    48181 taactgttgg tctcttcttc tgcccaggcg ctgatcttct cgatccattt agcctgattg
    48241 ctacggttaa acttgcgtcg atcaatacca gaagattcga tcagcgcatc cagttcaccc
    48301 actgcgtcgc gccactgctg ttttaccgta tcaatacgcg ccacaatttg cgcgtggcgg
    48361 gaagccagcg tttcatcatc gggcggcggt gctttgataa ccggtgcttc gccttgcaga
    48421 taacgattaa tatcgcgcag caacgcctgc ggccctttcc aggtttcaaa gaccaccagg
    48481 gcaatttcac gcggcagcgg gtagcagtgg cgacgccaga aatcggcgca ggcctggtag
    48541 cgtagcagag actcatcttc aatcagctgc tgctcaaaca gcataccgga ttcaaaggca
    48601 ttcaggttga gcatgcgctg gcaaaaaccg tgaatagtaa agactgccgc ttcatccatc
    48661 tgccgttcgg ctaataacaa ccactgcgcg gcttgcgctt tatcgtcgat ctcttccagc
    48721 aggcgttcgt acagtggatt gtcggtggtt tcacgcagac aggcgatgcg caactcgtgg
    48781 atattgctac ggatacgacc gcgcaattct gccgtggcag cctcggtaaa ggtgaccacc
    48841 agcagttctt caacggtcag cgggcgggga aaggcggcgg aaccgcctag tccaagtaac
    48901 aggcgcaaat agagcgccgc aatcgtaaag gttttgcctg tgccggcaga ggcttcaatc
    48961 aggcgctcgc cctgtaaggg caagcgcaaa ggatctagtg tctcggcgac atcactcatt
    49021 cttttcactc atcaggggca ttgtttgctg caacgcgctg acgttctccc acactttcca
    49081 gccttcagga tgcacatatt cggctttccc gttctggctg ccggaaatct gcgacaggat
    49141 agccatgcct tgcggctcga ccaccgcctg atggaagaaa tcagcaagtt tttgcggcgt
    49201 cagcagtttt atctgggcca cgattttatc acgcgaatcg aagcgcatat tgccgcgatc
    49261 gaaatcttta cttaacttcg atgcttcttc gccgagcgtt tgcggtgcct gcagcatctg
    49321 ggtaattacc gcctgctgga tttgcgcaaa ctcatctggc ttcatcgctc gcaattttgc
    49381 ctctgcggtt gggaaaaacg ccttgtaacg ctcccacaag aatgaaggct gtttatcatt
    49441 gctttgcaaa aggaagccca tgccccactg acgccccacg ctcattggaa acgcaaacac
    49501 ggcatagccc aattgttctt cggtacgcaa ctgattgtag aaccacggct gtacgatctg
    49561 ccccaacaga gagctatagg ctgagctggt gtattcatcg tagccagtcg gtacaaatac
    49621 cgctgccagt gcggagtcgg tgctgttacc ggctttttca aagatgacgg attgtttttt
    49681 atcgaccact acatctttgt ttcgacacca ctctgaacca tcagcgccca actgtttttg
    49741 cacatcgcgt gccagcgttg ttgcctgggc ctcggtcatg ttgccgataa ccataaactc
    49801 tggtcgagcc cctgatttta aggcgtcgcg ataggccagc acctctttca acgtaatgga
    49861 gggcaaaatt ttacgccgtt catctcgcga gaagtacggc acttgcgaga gcatctgcgc
    49921 gggcataatc gcctgctcaa acgctttacc cttttctgcg gaatccatca tctggttata
    49981 ccaggacttc gcctgctcaa gctgatcttc cgtagcggta tagctaaagt acccctcgag
    50041 caatgcctgg aacagctgcg gcagacgctg ggtgtaacca ttagcattaa ccataaggcc
    50101 gttgttagcg ttggtggaaa aacttatgcc accaaccgac gcctggttgc ttaactgatc
    50161 aagcgccagc cctgcgagat aatcattgag cgcaaacatc acctgattgc gggcgctgtc
    50221 catggctttc ggattacgca aaatcaggct gacatcagct ttgggttcgc tggaaaaata
    50281 acggcttggc gcatacacca cgcgcagatt cgactcatca acaatcagct ctggatggtc
    50341 atatttcttc tctgacttaa tcagcgagaa atcgtcagga atataggggt taagctctgg
    50401 caaagagagc gcaatgtcgg cggctttttt ctgccagtcg gcgaaagttt gtgcgctgat
    50461 tttatcgacc tgatacggcg catcgacaaa gtaagccgtt ttgttgtgcg gctctttcgg
    50521 gctgatatac cagatacgcg cattctgcgg cgtcatcatc gccagacgtt cctttactgc
    50581 tttagcatcg taccgatcgg caatattgac tgcatccagc gtatgctcaa caggaacgcg
    50641 aatcatggta tctgccagcc attcgacgta atccatatca cgggtgattg acggataacg
    50701 gaagtcgata tccagcacat tcgccagttc atcgaagtat tgtttatcga tccctttttc
    50761 acgtaacaaa ttgaggtagc taaaaatagc cgccacaacc tgatcgcgat tcgccagacc
    50821 tttatcggtt aaagacgcag agatcgctaa tacgccgctg ttgccgttga cgataggatc
    50881 ggagttggcg ctaatgccct caactaatcc ctgcttttgc agccagtcgg aaagtgtacc
    50941 tgggctgcga ttaccaatca gataggtaat caattcatcg gttttgctac ggaatttcgc
    51001 cgagttgtta tcaatacgaa actctacgcg caacacttta cgcggcagcg ccgggacgta
    51061 atgaatgata atgccctttt gcgcgtcggt gactaccggc acggtgattt ccggtttttt
    51121 gctctctttg ttcggcacgc gaccaaaggt gtccgccgcc atttttgcca actccggcag
    51181 cggtttattg ctgtaaataa ccgccttcat cagattggcg gaatagtact tctcgtggaa
    51241 atctttcagc gcctgctgca ccggattacc aggtttgtcg cttaaagttt cgaggttacc
    51301 accagaaaac tttgaaccgg ggtgtgccgg gttaatggtt tctgcgctga cctgtgccat
    51361 gcgcatcccg tcacgcgtac gcgccatggt taattcagcg ttcactgcat tacgctcacg
    51421 ttcggcgtat ttcttgtcga gcaagggttc tgcaatagca tcagccaggc ggtctaccgc
    51481 accaggcaag gcgtcgttct caacttccag atagaaagcc gtgcgatacg gcgcagtgct
    51541 ggcattgtga ctaccgccgt gcattttgag atattcggcc agactgtcag cctgcgggta
    51601 ctttttcgac cccatcagac tcatatgttc aaggtaatgt gccagcccct ggtacgcctc
    51661 gggatcttcc agcgacccaa cgggcaccac cagcgccgag agcgatttaa ctgcctgcgg
    51721 atcagaaacc agcaagacca ccataccgtt atccagacgt atagcctgat actggcggtt
    51781 atctttatca cttttacgga tggtttcctg aatcggctgc catcccgttt ctgcctgact
    51841 taagggtgcc caaagggcaa ctaacaacaa taatgctttg aaccaggtgc tgcggggcat
    51901 tcacggacct cataatcaac ttaattttct gtccagattc aacacgttaa cgcaaatcat
    51961 ctgccagaat ttaatcttgt gctgcacgag tcagcctatg tttatataac catcagtccg
    52021 tgactggtgc gcatcataaa gtaagcggat aaattgcgca atttttatac agcactcatg
    52081 actgattaaa gcgaaacagc ggtaacagga aacgttgcga ctgttcaacg atggcctcca
    52141 ttgtctctgg tgttaattgc cgccagagcc gttgatacca gatatcatca ccttcgccac
    52201 gcaccatcat gttgccttcg taagcctgca ggaatttcgt acgggctttt tgcaacgtgg
    52261 aatcgtcatc cagcatggca tcgttttgcg cgtcataaca ggtttttagc cacgcgccgc
    52321 cactttcagg taacaccagc aatggcgcgg acatcccttc acgatacccc tcaatcagtt
    52381 gtgagaggta atgcaaagcc tgttcggctg caagcggcgg aaaacgccac tcgccgtctt
    52441 tgcgtagaaa aaggcgactt tcaccattac caccgctggc acagtagaca aggtgttcca
    52501 gccaaagttg cattccctgc gccacactta ataaagaggg acgccagcgc aacaagccat
    52561 ccggctgcac ctgcggcaac cagccagtta tctgcacacc gttgcaggcg agatcaattt
    52621 ccatactctg ccccggctgg cgacaggcaa tgactctgtc ggcaagctgc tgcatctcct
    52681 ggcactgtgt ttcccagaaa atttcaccaa aagcgccata cggtaaatcc cctgccgccc
    52741 ggaagcggcg gaacaagcgt tcggcatcat cctgctcaac cagtgcattc aataactgct
    52801 gattgatttg ataacgacta agcccttcaa gaataaatgg ctcggtgtcg gggatttcgc
    52861 tgtcttcagt acggaagttc acctgcaaac gcatctggaa gaacgcccgc accggatgtg
    52921 cccagaatcg ttgtagcgtt tccagcggca cggtttccgg taaggtaaac ggcagcggct
    52981 gaacaaattc agaatgtgct ttaccagcct ggctggccgc aggtagccat tcacgagcat
    53041 agctttgtcg ttcgcctggc tggtagtttt gtggatcaaa cggcatccgg gtatggaggc
    53101 aagtaagatg cgcttttacc cttgcctcgc tttcatcaca gttgagcgct tcatcgcccg
    53161 gtagataatg actttgcccg atgtagtcga tcagttcctg caccagtacc gacgggaaac
    53221 gttcactgtt atcctgaatg gagcgcccga tatagctgat ataaagtttt tgttgcgcgg
    53281 aaattaacgc ttccaggaac agatagcggt catcatcgcg acggctacgg tcgccacgct
    53341 tcggtttctg gctcatcagg tcaaagccca atggcgcaag ctgacgtgga taaacgccgt
    53401 cgttcattcc cagcaggcaa accactttga acggaattga acgcattggc atcagagtac
    53461 aaatgttaac cggtccggcg agaaaacgct ggctgatacg ttcttgatcc aggcgctgtg
    53521 ccagttcatc acgcaatagt gacagcggca ccgcgtcgcc atactgcgca cctaaacctt
    53581 cggcgataat cgcctgccat tgttgttcga tcagcgtcat cgccgcttcg gtttccgcat
    53641 ccggcaggaa gaaggcgttg agcatatcgc gacaaaccgg caaccactct tccagcggac
    53701 gctcctgtgc cagcccgcga cgccagatgt ttagctgcat tagcagggaa gccagatgcc
    53761 ccaccagttc tgcaattaag ccgctcgatt catcataagg tagaaccgat tgccactcgc
    53821 cctgcgcgct ctccatcgcg taacccagca acatgcgcgt caggccaaac cgccaggtgt
    53881 gttgaccggt agcggggagt tccagctcgc gaacgttgtc gtcatctatc ccccaacgaa
    53941 tgccggattc gttgacccac tggcgtaaat aacgcagccc ttcttcggtg atgtcaaacc
    54001 gcgccgccag caccggcaca tccagcagcg ccagcacatc ctctgacaca aagcgactgt
    54061 caggcagtga taacaggctg ataaacgctt ccagtaccgg atgtgactgc cgcgcacgac
    54121 ggtcggaaat ggcgtaaggc aggtaacgat ccgcaggtgc gctaccaaac actgcctgaa
    54181 taaacggact gtagctgtcg atatcagcca ccatcacgat gatgtcgcgc ggagtaagtg
    54241 tcgggtcttc ctccagcatc gccagcaggc gatcgtgtaa aacttcaact tcacgctgcg
    54301 ggctatggca aacgtggaag gtgatactgc tatccagtgg atcaagcggg cgtttgttat
    54361 cgctacggga aaactcttcg atgttcacac cagcaacggc gcggttttcc agttccagaa
    54421 tgtcagactg aatgttatgc agcaggttat ctggcatcac atcgacaaag gcgtccagct
    54481 cctgactgct atccagatcg gaaaggagat agatatagtc gcgccccaac ttgccccagg
    54541 aggccagtag cgggttgccg acatcctgtt caccgtcgct gttaaagagc tgtccggcat
    54601 tttcgctatc gcgaaatagc ggcaattcgc gatcttcaaa actgtgtcga cgctgacgag
    54661 tcagcagttt cgccagataa gcaggatctt taatatcgcc ccagtaataa cggcaggggt
    54721 tggtaaacag gagatggatt tcaatatgtt tacccagcgc ctggagcgcc tggagataaa
    54781 caggcggtaa cgcggaaata ccgcatataa agacgcgcga aggtaacccc ggcgggcagg
    54841 tcgtcgcgga ctccagcgtt tcgataaagc gctgatagag attggcgcgg tgccagcgcg
    54901 gttgcccgag ttgatgggta tattccacca acgccttcca caacggcgct tgccaggcct
    54961 gtgcttctcc cagcccttca accaaatgtc ctgtttccca ctgtgccagc cagtccggac
    55021 gatagaccag atactggtca aacaggtccg ccgcttttga agaaagctgg aacagttttc
    55081 gcttgtcgct atcgtcagtc agataatgcc gcaacagggt aaagtcttcg cgatccagca
    55141 attgcggcag cagagtcatc agtttccagc tcatgctctg tttgttaaag gcgctctctt
    55201 tggggatttc cggtaacacc cggacgaaca tatcccagat aaagctcgca ggcagcggaa
    55261 aatcaatgtt tgccgcaata ccaaactttt gcgacagggt catttgcagc cactgcgcca
    55321 taccggtact ttgcaccaga atcatctctg gttcgaaagg atcgtccagc cgttcgcgtt
    55381 cgacaataaa ctccatcaac gcttccagca cgtccagacg attggaatgg tagaccctta
    55441 acatagcggc tcctgactac tgacgattcg ggcaatgcag gcgggtcatc tcgccttctc
    55501 tgcccccggg tgaaactagc gtaacgctga tgctgacaca tcccgcctgc gatgtctgca
    55561 ttcggttgac ctgccagttg gcaggtggcg aaatcgcgcg cagttgcgtt tgctgccagc
    55621 catgccgcca gagctgttgg tactggtttc tgctggcaag actgttcatt aatgttcgct
    55681 ggaaacccga taacgcagtg acaatcatca ccatcaacac catcgccaac attacctccg
    55741 gcaggctaaa gccttgttga ttcttcaggg aagctgacat aacgccctct ctttcagcgg
    55801 acaaaaatcg ctccagccgc gtggcgaaaa aacaatgttt ccatcaatga cttcgcctgt
    55861 tcgccacaac gaaacgcctt cataatcggc aatcaataag gcttcattat ctgccagtaa
    55921 acgcaaacaa acctgggcat cggttccagc gtactgcgag cactgaactg ctggctgcgt
    55981 ctgccaggag tgcatttttc cccacgccag cgccgactga acgatggcct ggcggcgcaa
    56041 tgactgactt tccatgctca cgcgagaggc aaaactgcga tcctgttggc tcattccttg
    56101 taatagcaag ctacctaaaa ccagcagcat caggaccaga gccagtgacg aaacaccctt
    56161 ttcgcggttc acaggttgaa tcctgtcacg ctatagctgg cattcaccac ggtttgcggt
    56221 tcagacttac tggcagcacg catattaacc gtcaacaccg gcgagaagcc gctgacatcc
    56281 tgatgtacga cctgaaaagt gtcgataatg atggcatccg gattagtgac tttatcccag
    56341 cccttacctt cacaggatgt cgcaccgcgt agcgtttcca gcacatgctc cttcagacga
    56401 aatccaatct ggtcagactc ttttaccggt tcgcgatccc agataccgtt actgttcgca
    56461 tcccactgca caatgataca gtcaccctgt ccgacaattt ccagcccttc gccggtacag
    56521 ctgccatgac aataacccgc cctctggaga tgcttcgcga cggtaaatac ccgcagccag
    56581 atttcatctt ccagcgccag cttacgggtg ctcgttaaac tttcacgctg taacgcaggc
    56641 agaaagcgtg cagcccccag caacaatacg ctactgatcg ccatagcaat caacacttcc
    56701 agcagagaaa aaccttgctc ttttacaggc atccttctgt ttctccttgc tgacaaagcc
    56761 ggagtcttcc ccacggcgaa accaccagcc accactcgcc cgttgagttt ttgaagcgaa
    56821 tatgcccggc ccatgcggta ttgcgcaggc caaagaaagc aagcgaaggt gtcaggtcgc
    56881 tcatttcgac ttcgggccag cgtggcacaa agaccaatgg tgaactgcca tgacaggtat
    56941 tggccccagc agcggaactc acaaggcacc ataacgtccc ctccctgata acgctgatac
    57001 tgtggtcgcg gttatgccag ttggcatctt cacgtaaata gagcaaatag tcccgcgcct
    57061 ggctggcggt ttgccatagc cgttgcgact gctgccagta ttgccagcca tagagtccac
    57121 ttgcgcttag catgaccaaa atcagcatcg cgaccagcgt ttcaatcagc gtataaccac
    57181 gttgtgtttt catgccggca gtatggagcg aggagaaaaa aagacgaggg ccagtttcta
    57241 tttcttcggc gcatcttccg gactatttac gccgttgcag gacgttgcaa aatttcggga
    57301 aggcgtctcg aagaatttaa cggagggtaa aaaaaccgac gcacactggc gtcggctctg
    57361 gcaggatgtt tcgtaattag atagccaccg gcgctttaat gcccggatgt ggatcgtagc
    57421 cttcaatctc aaagtcttcg aaacggtagt cgaagatgga ttcgggttta cgtttgataa
    57481 tcaacttcgg cagcggacgc ggttcgcggc ttaattgcag atgagtttga tccatatgat
    57541 tgctgtacag gtgcgtgtcg ccccccgtcc agacaaaatc gcccacttcc agatcacact
    57601 gctgcgccat catatgcacc agtaacgcgt agctggcaat gttaaacggc aggccgagga
    57661 aaacgtcaca ggagcgctga taaagctggc aagagagttt gccgtctgcc acatagaact
    57721 ggaagaacgc atggcacggt gccagcgcca ttttatccag ttcgcctacg ttccacgctg
    57781 aaacaataat gcggcgcgaa tccgggtcgt ttttcagctg gttcagtacc gtagtgatct
    57841 ggtcaatatg acgaccatct ggcgttggcc aggcgcgcca ctgtttacca tacactggtc
    57901 cgaggtcgcc gttttcatcg gcccattcgt cccagatggt gacattgttt tcgtgtagat
    57961 aagcaatgtt agtgtcgccc tgcagaaacc acagcagttc gtggatgatg gaacgcaggt
    58021 ggcaacgttt agttgtcacc agcgggaatc catcctgcag gttaaaacgc atctgatgac
    58081 caaaaatgga aagcgttccg gttccggtac ggtcgttttt ctgtgtgcct tcgtcgagca
    58141 ctttttgcat cagttctaaa tactgtttca tggttcctca ggaaacgtgt tgctgtgggc
    58201 tgcgacgata tgcccagacc atcatgatca cacccgcgac aatcatcggg atggaaagaa
    58261 tttgccccat gctgatgtac tgcacccagg caccggtaaa ctgcgcgtcg ggctggcgga
    58321 aaaactcaac aatgatgcga aacgcgccgt agccaatcag gaacaagcca gagacagctc
    58381 ccattgggcg cggtttacga atatacaggt tgaggataat aaacagcacc acaccttcca
    58441 gcagcagctc gtaaagctgt gatgggtggc gcggcagcac accgtaagtg tcgaaaatgg
    58501 attgccactg cgggttggtt tgcagcagca aaatatcttc tgtacgggag cctgggaaca
    58561 gcatggcaaa cgggaagttc gggtcaacgc ggccccacaa ttcaccgtta ataaagttgc
    58621 ccagacgccc ggcaccaaga ccaaacggaa tgagtggtgc gataaaatca gaaacctgga
    58681 agaaggaacg tttagtacgg cgggcaaaga taatcatcac cacgataacg ccaatcaggc
    58741 cgccgtggaa agacatgccg ccgtcccaga cacggaacag atacagcgga tcggccataa
    58801 actgcgggaa attgtagaac agaacataac caatacgtcc cccgaggaag acgccgagga
    58861 agcccgcata gagtaagttt tcaacttcat ttttggtcca gccgctgccc ggacgattcg
    58921 cccgtcgtgt tgccagccac attgcaaaaa tgaaacccac cagatacatc aggccgtacc
    58981 agtgaagcgc cacgggtcct attgagaaaa tgaccggatc aaactccgga aaatgcagat
    59041 agctactggt catctgtcac cacaagttct tgttatttcg ctgaaagaga acagcgattg
    59101 aaatgcgcgc cgcaggtttc aggcgctcca aaggtgcgaa taatagcaca aggggacctg
    59161 gctggttgcc ggataccgtt aaaagatatg tatatgatcc gcgctataac cctccgcgaa
    59221 tcagcccgcc catgccgcga cgctccataa aggctgcaac ctgatggcga acttcggtcg
    59281 ccagttgcgc ttccagacta cgctgcgcaa gattttctgc ttcggcataa tcaatgcgcc
    59341 gcagcaggta ttttacccgc gctacagaac gtccgttcat agacagatgg cgatacccaa
    59401 gcccaatgag gattgccacg cacatgggat cgcccgccat ttcaccgcac aaacggagat
    59461 cgattccatg tatttccgct tcccgggcga tcatcgccag agctcgtaac attgcaggat
    59521 gaagactgtc ataaatgttc gccacccggg tattgttgcg atcaacggcc agaatgtatt
    59581 gagtcagatc gttggtgcca acagagatga aatcgacccg ctttgccaga tgcggcagca
    59641 taaataccat tgacggcact tccagcatga tgccgatacg tggtttggga atttcgtaac
    59701 cgatcatctc ctcgacttca cgtccggcac gttcaatcag gcggcgtgct tcatcaactt
    59761 catcgaggct tgtgaccatc ggcaacagaa tattcaggtt gcccgtagcg gcattagcac
    59821 gcagcatcgc ccgcacctgg atcaagaaga tctccggctg atcgagcgta atgcgaatcc
    59881 cacgccaacc caggcatgga ttctcttcgc tgatcggcat gtaaggcagc tgcttatctg
    59941 ctccgacatc cagcgtacgc aaggtgacgg gtttatcatt gaacatttgc agcatcccct
    60001 gatactgcgc cacctgttct tcttccgacg ggaaaccact ttgcagcatg aatgggattt
    60061 cagtgcgata aagaccgatg ccatcaatac ggctgcccag tttttcttca tgttccgggc
    60121 ttaaaccagc attgagcatg acttttatac gctcaccgct ttttaactgc gcgggtaaat
    60181 tgacgtcatc ttccgccaga cggctaagct caatctcttc actaattagc cgctgatatt
    60241 cttgcagcag taccggctcc ggatcgacca gcaattcacc gcgatagcca tcaacgatca
    60301 gcgtcctacg atgcagcacc gaaggctgaa tatccgcgcc catcacggta gggatcccca
    60361 gcgcacgtac catgattgca gcatgggagt tggctgcgcc atcgcgcacg acaacaccca
    60421 ctaagcgatc ctggggcagc tcagcaagag ttgtcgctga cagttcatct gccaccagaa
    60481 tgaaacgttc cggccaggcg ttcggccctt gattagcgtc atcaagatga aacagcaatc
    60541 gctgacccag cgcacgtaaa tcgccagccc gctctttgag atagttatcg cttagcgcgg
    60601 caaactgttc ggcaaatttt tcaatgaccg tttttaccgc ccactctgcc accgagcctt
    60661 tatcaacctc ggcaaacaat tcgcgacgca gccgggtgtc cgaaagcagg tgcgagtaaa
    60721 gatcgaaaat agccgccgtt tctttttgtg ctccggcggc aaagcgtttg ctgtagcggc
    60781 gaaactcgtt tgccgcttct tccagcgccc cggtcagtcg ttcgcgttcc agagccggat
    60841 ccagcgttga tgcctgatac acctgttcca ttaaaggtaa cgtgacatcc tgccagcctt
    60901 cggcaatcgc cacaccaggt gctgccggta atgcgcggat tcgcgtctgg cgatattgcc
    60961 caaacaaggc agtcaactgc gactgagaaa gaatagctgc catctgggtg gcaagcgtca
    61021 ccaggaagga ttcttcactt tcgtcatact ggcgcaactc tcgttgctgt accaccagta
    61081 caccaagcaa ctggcgacgt tgaataattg gtacgcctaa aaacgcgcgg aaacgttctt
    61141 cttttacgga ggggatgtat ttgaagctgg ggtgcttttg cgcatctgca aggtttatcg
    61201 gttccgccag cctgccaacc aggccgacga tcccttcatc aaacgcgagc gttacagtgc
    61261 gaccgcgtgg ttttttcagc ccccgggtcg ccatcaggta gtaacaacgt cgatcatgat
    61321 cggcaaggta gaccgaacag acctcggtat ccatcgcaag acagatgtcg gtaaccagaa
    61381 tatttaacgc ctcattcagg cgtggtgcgc tggctacctt ttcgactatt tcgcgcaggc
    61441 gagtgagcat aattggcgtg acttaacctc ttttacgtcg ataagcagat gcgttttgtg
    61501 gttttggcgt attttcctgc agtgacatca ccacgctcgc gaactctttc attaccctac
    61561 ggtagacatc acgtttaaat gacaccacct gtctgaccgg ataccagtaa cttacccatc
    61621 gccagccgtc aaactctggt gtactgctgg tttgcatatt gatttctgca tcgccgctca
    61681 ccagctgcaa gagaaaccat ttttgttttt ggccgataca aaccggcttc gtgtcccaac
    61741 gcaccaaacg tttcggtaat ttgtagcgca accagttacg cgttgaagca aggattcgaa
    61801 cgtctttgcg gcttaatcct acttcttcaa acaattcacg gtacatcgcc tgctctgcgg
    61861 attctccggg gttgattccg ccttgcggaa attgccagga gtgctgacca aatcgccggg
    61921 cccacattac ctgcccctgg cgattacaaa tcacgatacc tacgtttggg cggtagccat
    61981 cgtcatcaat caccggacta cctcaaaata aagctttata tacgaatgat tgtttcatac
    62041 tccaggaaga cggtaaacca ctctctgcag ggcattacac actaataaca attgaataac
    62101 tcacagttat gtgcagagtt ataaacagag gaaggggtgg atagccgttt ttattcacct
    62161 tttctgtgga tagagttgtg aagaactacg gaattactac gggaaaaccc ggagcattct
    62221 gaataagccc gatatagaca ttttaaaata ttcattatta gtcaatgcat tacatgtaat
    62281 ttgagtaatg aaaaagagta attcgtgacc caggtcacac ctctcattta cgggttggcg
    62341 aaagatcaac caatgccgta tttatccaca gaatgtgcca ctaagttaag cactgaacca
    62401 ctaaaaactg gagtttcatc gcacgtcaag gctgtaaatg gaaacagtag tggaggtttt
    62461 tcacagttat cccagctttc tgtggataac atggtgtaag atcctgttta ttttcagtga
    62521 ccagatttgg aaaacctatt gcagtgttgc gcaactcgat taccggcagc ctaaaaagca
    62581 atataaatca gggaattgaa caacacatgt ggaaaaagtt acactgcgaa tattcggcac
    62641 ataattgctg tttgtttttt aatcaaggta tcatgacatg tcccaacctc gcccactgct
    62701 ctctcctccc gaaactgaag aacagttgtt agcgcaagca cagcaacttt ctggttatac
    62761 attgggagaa ctggcggcac ttgccgggct tgttacgcca gagaatttaa aacgcgataa
    62821 gggctggatt ggcgtgttac tggagatctg gctaggtgcc agcgcaggga gtaaacctga
    62881 gcaagatttt gccgctctgg gcgtggaact taaaactatc cctgtggata gtcttggtcg
    62941 tccgctggaa acaacattcg tttgtgttgc cccgttaacg ggtaatagcg gggtgacctg
    63001 ggaaaccagc catgtgcgcc acaagctcaa acgcgtactg tggataccgg ttgaaggcga
    63061 gcgcagcatc ccgctggcgc agcgtcgcgt aggatcaccg ttgctgtgga gcccgaatga
    63121 agaggaagac cgacagctac gcgaagactg ggaagaatta atggatatga ttgttctcgg
    63181 tcaggttgag cggatcaccg ctcgtcacgg ggaatattta cagataagac cgaaagcagc
    63241 gaatgcgaaa gcgcttaccg aagccattgg tgcccggggc gaacggattc tgacgctgcc
    63301 acgcggcttt tatttgaaga agaatttcac cagtgcacta ctggcccgtc attttctgat
    63361 ccagtagcca tcgctttgac ctgccgcttt ccgggcatat aattaccgct tcattttttt
    63421 ggcagggctt tttagatgtt atttgcatgg ataaccgatc ctaacgcctg gcttgcgctc
    63481 ggtacgctga cgctgctgga gatcgttctt gggatcgaca atattatttt cctttctctg
    63541 gtggtggcaa agcttcccac agcacaacgt gctcatgcgc gccgtctggg gttggcggga
    63601 gccatggtta tgcgtctggc gctgctggca tcaatcgcct gggttacgcg cctgacaaat
    63661 ccgcttttta caatattcag tcaggaaatt tctgcccgtg atttgattct gcttctgggt
    63721 ggcttgttcc ttatctggaa agccagcaag gaaatccacg aatccattga aggtgaagaa
    63781 gaagggctga aaacacgcgt ttcatcattc ctcggcgcta tcgtgcagat tatgctgctg
    63841 gatattatct ttagcctcga ctcggtgatt acggctgtgg gtctgtcaga tcacctgttt
    63901 attatgatgg cagccgtggt gattgccgta ggcgtgatga tgttcgccgc gcgctcgatt
    63961 ggtgattttg tcgaacgcca tccttcggta aaaatgctgg cgctctcttt cctgattctg
    64021 gtgggcttta ccctgattct ggaaagtttc gacatccacg taccgaaagg ttacatctac
    64081 ttcgcgatgt tcttctctat tgcggttgaa agcctcaact tgattcgcaa caaaaagaat
    64141 ccgctctgat actccgtacg ctctcctgcg ggagggcgta ctcactcctt ttaccctcac
    64201 agtttaagat tttcctgctt tcaagatata taacgtcggt ttataaacag actattatca
    64261 taggtaggcc aaatgaggat aaccggatga aaaaatgggc agtaataatt tctgcagtcg
    64321 gactggcgtt tgctgtttcc gggtgttcca gtgattacgt catggcgacc aaagatggcc
    64381 gtatgatttt gaccgatgga aaacctgaaa ttgatgatga taccgggctg gtgagttatc
    64441 acgatcagca aggtaacgcg atgcaaatta accgtgatga tgtttcgcaa attattgaac
    64501 gttaacaaat aaggtcagca tccggctggc cttaagattt ttctctttcc cttttccctt
    64561 ccctctgcca tttttatatt ccttatgtcg tgattataaa aaggaaacgg ctatgcaata
    64621 tcaccgtata ccccacagtt cgctggaagt cagcacgctg gggcttggca cgatgacgtt
    64681 tggtgaacag aacagcgaag ccgacgccca cgcacaactc gactatgccg tcgctcaggg
    64741 cattaacctt atcgacgttg ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg
    64801 gttaaccgaa acctacgtcg gcaactggct ggcgaaacat ggcagccgcg aaaagttaat
    64861 tatcgcctcc aaagtgagcg gaccgtcgcg caataatgac aagggcatcc gcccggatca
    64921 ggcgctggat cggaagaata tccgcgaagc gctgcatgac agcctcaagc gcctacagac
    64981 tgattacctc gatctttatc aggtgcactg gccgcagcgc ccgaccaact gcttcggcaa
    65041 actcggttat agctggacgg attctgcgcc tgcggtttcg ctgctggata cgctggacgc
    65101 actggcagag taccaacgcg cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc
    65161 atttggcgta atgcgctacc tgcatctggc ggacaaacac gatctgccgc gtattgtcac
    65221 cattcagaac ccttacagtc tgttaaaccg cagttttgaa gtaggtctgg cagaagtcag
    65281 ccagtatgaa ggggtcgaac tgctggccta ttcgtgcctg ggtttcggca cgctgaccgg
    65341 gaaatatctc aatggtgcaa aacccgctgg cgcacgtaat acgctcttta gtcggttcac
    65401 ccgctatagc ggtgagcaaa cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg
    65461 tcatggcctg gaccctgctc agatggcgct cgcgtttgta cgccgtcaac cgtttgttgc
    65521 cagcactctg ctgggcgcaa ccacgatgga tcagctgaaa actaacatcg aaagtttgca
    65581 tctggagtta agcgaagacg tattagctga aattgaagcg gtgcatcagg tttatactta
    65641 tccggcacca taagattaac gccggttaaa accggcgtta aatattaatg acggcgctgc
    65701 cagatccaca gcgccgttat tgccagcgca aacagcgcac cgaagccaat gccaatgggc
    65761 acgaccggga tgcctatcat taccgccagc gagtaaatgc ccagcatcaa caacatggcg
    65821 ctgttttcgc caaggttttg tactgcaatc gcattccccg ccccgacgct ttttttaccc
    65881 cgctcctgta gcaacgcatt gagcggaacg acaaaaaagc cccccatcac gccaatcagc
    65941 atcaacaagg cataggctgg cagcagctcg tgttgcaggg aaaaaatcag taccaccacg
    66001 ccaatcaaaa tcccggctgg catacagcgt gacacggttt ccagcgtaac taacttcgct
    66061 gccgcacctg cgccaaccac gataccaatc gctaccatcg cgttgagata ggtgggcgta
    66121 gcgttatcgg taatgcccag cgccaccggt acccacagca ccaacaggaa acgcagcgtg
    66181 acacccgctc cccagaataa actggtgccc accagcgaaa aacgcgtttc accattgcgc
    66241 catagcgagg tgcaggcatt caggaaactg cgggtcatgt taatgagatt ccaggactgc
    66301 cccggacgcg ccgccgccag tttgggaatg tagatattgg caacgaccgc accaccgtag
    66361 gccagtgcgc atgcggccag ggcgacgagg acatgccagt cagccagcac accaccggct
    66421 acggaaccga gcaaaatcgc cgctatggta gaagcttcca ttaaaccgtt agctttcact
    66481 aacttactac ccgtggttaa ttcgccgaga ataccgtatt tcgccggtga ataggctgca
    66541 gcaccaacac ccaccagcgt atagccgaga aacggattga taccaaagca gatactggct
    66601 gcgcccagca gcttcaggcc gttggcaaac atcatcaccc ggcctttggc gaagctatcc
    66661 gccacctgcc cgacaaacgg cgcaaaaaga atgtaagcac ctacaaacac catttgcagg
    66721 atgggctggc tccactccgg atagaactgc gctttcagta acgccagagt ggcaaacagt
    66781 agggcattat cgccaaacgc agagagaaac tgcgccacga taaccgcttt catccccttc
    66841 gaccacaacg aagtgttagt gtgcactgac tcactcatcg tgttgttccg cttcgtctac
    66901 ccagcttttc aacgtgacaa agtcaggttt gccgctgcca agtaatggca tctgtttcag
    66961 atagcgaata tcgcgcggta cagcaagctc cggcacgccg tgctcgcggg catactgttg
    67021 caacttatcg cgcgtcagtt cgttatctgt ggtgaaaagc accagtgcct cgcctttgct
    67081 ggcatcgctc ttaatcgcag tggcatggac tttatctggc gaaacaccaa gtgccagttg
    67141 ttccaccatt tccagcgaca ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc
    67201 ctgaatctgc acaaagccct gctcgtcaaa acgcacaata tcgccagtgt cataccagcc
    67261 gcgctccatt tcgccgcgaa cattctcggc ggtgggcact tccagtacac ctggcttctc
    67321 cacccgcaga tagccgttca ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc
    67381 gataccaggg accgacaaca ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc
    67441 gggtttcgcc gccatcggta cgttgataga aacgacaggc gcgcattcgg tcacgccgta
    67501 gccttcaagg atgcgcaggc caaatttatc ctgccaaagc tgtttggtac tttcttgtaa
    67561 tttttctgcg cctgccacca catagcgtag acgatagaag tcatacgggt tggcgaaacg
    67621 cgcgtagtga ccgaggaaag tcgaggtgcc gaacaacacg gtgcaactgc ggtcatacac
    67681 caactccggc acaatgcggt aatgcagcgg gcttggataa aggaacactt ctgcacctgt
    67741 aagcagtggc gtaaacaggc ctaccgtcag cccaaaggag tgaaacagcg gtaacgccga
    67801 cataaagcga tcgttggtgg tgaagtcggc aatcgtttta atctgctcga cattcgccag
    67861 aatgctttta tggctatgga cgacgccttt cggatggcct tcagaaccgg aggtaaagag
    67921 gatcagcgcc tcttcttccg gctgctgttt aacctgtgcc agacgcggca tcagcaaatg
    67981 agcgaagatc catactttgt cggcagtggt gacatctgct tttaaatctt ccagatagac
    68041 ccagcgcacc tgagtaagtt gctccggcag atgccagagt ttgcctttat cgagaaactg
    68101 gcgggaagtg aagatggttt tgatttcagc cgccgtaata gcactggtca gcccttttac
    68161 cccggcagtg tagttcatca ttgcgggcat gcggcgacgg gcgatggccc caaaaatcac
    68221 tgccgcactg atgcctgcat tgggcagcat taagccgatg cgttcgcctt caacactgta
    68281 tttttcaagg atgcgtccaa caaacagcgt tttcgtaagc aatttgcgat aggagtctgg
    68341 ggtaaagttg acgtcttcga cacatttctt cccggctccg aagcggtaca ttgcactcag
    68401 taaagattcg tacagcgttt cacgcgggcg caccgccatt cgcgcttcca tcattatttg
    68461 atgcagcatt tcgccagcga ttttgcgacg gtcacgggca cgcggcgcat ccggcatcgc
    68521 cacctgcgtt ggtggcaaaa tatgcagagt aatttgcggg aacaagcgac gtttaaccag
    68581 acctttcagg cggctgaagt gcgtaagttc cgccccttca atacgcacag gaataaccgt
    68641 tgcaccagac ttcgccgcga caaaacccgc gccatcgtag attttcatca gcgagcctgt
    68701 agtggtgatg cgtccttcag ggaaaatcac cactggtcgg ccctgttcca ccagacgtac
    68761 cagatgttta atagccatag gttgcgtcgg gtcgagagga acaaagtcga taaatgattt
    68821 cagccaacgc atataccact gttggcttat tgaggtgtaa acggcaaaca ctggacgcac
    68881 aggtaaaaac agtccaagca aaatgccatc aataaaagag acgtgattag gcgtaattag
    68941 aacgcgctcg cccttcagtg cctgggggtc acccgtaacg cgaacgcgat acaaaacacg
    69001 gcacaaattt cgaaaaaagc taaaaagcat tccaactccc tttgctctga ttcagtaaaa
    69061 gcgaatggag ggagattaca cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa
    69121 aaaaacctgc gcatccgcgc aggttggtgc aagagacagg gtacgaagag cgtaccgaat
    69181 aatctcacca atcaatacct ctgggatctt gattgtggtc tgcacgacga ctcttcgcca
    69241 gcgagaaaac gcaaaggaat gaagggaaat gcaacgaggt gtgtaaattg tcggttactg
    69301 ttacagattg atgaccggca aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat
    69361 gtgctcaacc cgaagttgac ttcacctatc aatacctctg ggatcaccac tttagcaacc
    69421 tgaagccaaa cgccaccagc ggtcaatcgc aacagcgttt cgcaaagtgt aaccaaaggt
    69481 ttgcaatctc tttttttgtc ttacttattt gcgtttggct cacattccca cgatgaaaac
    69541 acgccacccc ttgaaccaac gggcgttttc cgtaacactg aaagaatgta agcgtttacc
    69601 cactaaggta ttttcatggc gaccataaag gatgtagccc gactggcagg cgtttcagtc
    69661 gccaccgttt cccgcgtcat taataattca cctaaagcca gtgaagcttc ccggctggct
    69721 gtgcatagtg caatggagtc tcttagctat cacccgaacg ccaacgcccg tgcgctggcg
    69781 cagcagacca ctgaaacggt cggtctgatc gttggtgatg tttccgatcc gtttttcggt
    69841 gcaatggtga aagcggtcga acaggtggct tatcacaccg gtaatttctt attgattggc
    69901 aacggttacc acaacgaaca aaaagagcgt caggctattg agcaactgat ccgccatcgc
    69961 tgtgctgcgt tggtcgtcca tgccaaaatg atcccggatg ccgatttagc ctcattaatg
    70021 aaacaaatgc ccggtatggt gctgatcaac cgtatcctgc ctggctttga aaaccgttgt
    70081 attgctcttg acgatcgtta cggtgcctgg ctggcaacgc gtcatttaat tcagcaaggt
    70141 catacccgca ttggttatct gtgctctaac cactctattt ctgacgccga agatcgtctg
    70201 caagggtatt acgatgccct tgctgaaagt ggtattgcgg ccaatgaccg gctggtgaca
    70261 tttggcgaac cagacgaaag cggcggcgaa caggcaatga ccgagctttt gggacgagga
    70321 agaaatttca ctgcggtagc ctgttataac gattcaatgg cggcgggtgc gatgggcgtt
    70381 ctcaatgata atggtattga tgtaccgggt gagatttcgt taattggctt tgatgatgtg
    70441 ctggtgtcac gctatgtgcg tccgcgcctg accaccgtgc gttacccaat cgtgacgatg
    70501 gcgacccaag ctgccgaact ggctttggcg ctggcggata atcgccctct cccggaaatc
    70561 actaatgtct ttagtccgac gctggtacgt cgtcattcag tgtcaactcc gtcgctggag
    70621 gcaagtcatc atgcaaccag cgactaaccg cagttaaagc aattccagcg ccagtaattc
    70681 ttcgatggtc tggcgacggc gaatcaaccg cgcctgacca ttatcaaaca gaacttctgg
    70741 taacagcgga cggctattgt agttggatga cattgatgcg ccatatgccc ctgtatcatg
    70801 cagtaccaga taatcacccg ccttcacttc cggcaaggcg cgggtttcaa catttccccc
    70861 ttcctgctgg gtaaagacat cgcccgattc acataatggc ccggcgacga cggtttccac
    70921 cgttggcgcg tgttccagag aacgaccatc agctgccagg gcactgatat ggtggtaact
    70981 accgtacatt gccgggcgca tcagatcgtt gaacccgaca tcaaccagca caaagtggcg
    71041 gctacccatt tgtttgacgc tccgcacctg agtaattaac acgccagact gcgctaccag
    71101 aaagcgaccc ggttcaattt ccagtttcac agggtggccc aaatggcgag cgatttgctc
    71161 acgcgcggca ttccacagac cataataatg ttcggtatca accgcctctt caccctgttg
    71221 ataaggaata gaaagcccac cgcccgcaga aatagcctgt aaatcctgac cgaattcgat
    71281 gacctgacgt accatagcac cacacacctg ttccagatgg gcataatcaa cgccagaacc
    71341 aatgtgcatg tgaatgccga ccagctgcaa atgatgacgt tgtatcacgt ccagtgcggc
    71401 gggcagatcg gtgtaccaga taccgtgctt gctgttttcg ccaccggtat tggttttttg
    71461 gctatgtccg tgaccaaacc ccgggttaac gcgcagccat acccgatgcc ctggcgaaac
    71521 ctggcccagt tggtcgagca tatcaacaga acccgcattc accggaattt gcaattcact
    71581 gacgcgttca agcgtcgcct gatcgataac atctgccgta aaaacaatat catcggggtg
    71641 cgtttgcgga ttgtaacccg ccgccaacgc acgctctatt tcgcctaacg agacggaatc
    71701 cactttcacg ccctgctcac gcattaagcg caaaatatga atattggaac aggctttctg
    71761 tgcaaagcgc accacatcaa actgtttcag cgctgcaatc tgccgacgaa taatttgcgc
    71821 atcgtagacc cacaccgggc agccaaattc agcgggcaaa cgcagcagat tttcggcggt
    71881 gagatcggta tcggtgctga acagtgaatg tggcataaca aactccagat aagtgctttt
    71941 ttatgattac gccacatcat aaaaagaata aaaaatatcg atttatgtcg agtctatgca
    72001 aaaatgatat ggattaccgg attgcgagag agcgctaatg gccgccgtta acttacgtca
    72061 tattgaaatt tttcatgcgg taatgaccgc cggaagcctg actgaggcag cacacctgct
    72121 acacacctca cagccaaccg tcagccgcga actggcgcgc tttgagaagg tgatcgggct
    72181 aaaattgttt gagcgcgtac gtggacgatt acatcctacc gtgcaaggac tgcgtctgtt
    72241 tgaagaagtg caacgatcct ggtacggact ggatcgcatt gtcagcgccg cagaaagtct
    72301 gcgcgagttt cgccagggag aactgtctat tgcctgcctg ccggtctttt cgcaatcttt
    72361 tttaccgcag ctcctgcaac cctttctggc acgttatccc gatgtcagct taaatatcgt
    72421 gccccaggaa tcaccgctac ttgaagagtg gctctcggcc cagcgtcatg atttaggact
    72481 cactgagaca ctccatacgc ctgcgggaac agaacgtacc gaattactct ctttagatga
    72541 agtgtgtgtg ttacctccgg gtcatccgct ggcggtaaaa aaggtattaa cgccggatga
    72601 ttttcagggt gagaactaca tcagcctttc ccgtactgac agctatcgcc agttgctgga
    72661 tcagctattt actgaacatc aggttaaacg acgcatgatc gtagaaaccc acagcgccgc
    72721 gtcagtctgc gcaatggtac gggcgggggt aggtgtttcg gtggttaacc cgctcaccgc
    72781 actggattat gcggcaagcg gtttagtggt gcggcggttc agtattgcgg ttccgttcac
    72841 cgtcagcctg atccgccccc tgcaccgccc gtcatcagcg ctggtgcagg cgtttagtga
    72901 gcatttacaa gcggggttac cgaaactggt cacttctctt gacgctattt tgtcgtcagc
    72961 tacgacagca taaaagcgac agcatcctcg gcatggatcg ccgcagtatc aaacacaggc
    73021 aaaacactgc gctcttctgg caccagtaaa ccaatttctg tgcagccaaa aatgacgccc
    73081 tgtgcgccct gttctgcaag gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg
    73141 gtaaattgcc ccagacacag ttcttcaaaa ataatctgat taattttcgc ccgttcatcc
    73201 gcttcaggaa taagacaatt gatggaaaat tgttccgtca gccgcccgcg ataaaaatcc
    73261 tgttccatgg tgtaacgcgt acccagcagc gccacacgag tcattccggc cccggtaatt
    73321 gcacgtccgg tggcatccgc aatgtgtaag aaaggcagag tgcaacgtga ctcaatggca
    73381 tccgccactt tatgcatcgt attggtacat aacacaatac cttctgcgcc cgcccgctgt
    73441 aagccaagcg ccgcctcagc cagaatgtcc ccggttttat cccattcccc gcgacgctgg
    73501 cactcttcta tttcatgaaa atcgacgcta tgtagcagca cttgcgcaga gtgaagccca
    73561 ccaagccgct gtttaatgcc ttcatttatc agacgatagt aaggaatggt ggattcccag
    73621 ctcattcctc ccagcaaacc aattgttttc attcctctct cctgatgtgt gttaccgcaa
    73681 caaggctgaa taaggaacga gacaaacgcc tcaacggcca ggtgcccgat ctctattaac
    73741 gaaaaaaggg ccggatgtac agcacatccg gcccgtgaaa tcagacgccg atatttctca
    73801 acttctcgcc tgccatcagt ttgcgttcga tatgttccag cgtgacattt ttggtttccg
    73861 gaatgagcca gaaagtgatg cccacaaacg caatgttcag cgcagtgtag agccagaacg
    73921 taccggcagc gccaatgcta tcaagcagtg tcaggaaggt cgcgccgata atcatattcg
    73981 acacccagtt tgtcgtcgtc gaacaggtaa taccgaaatc gcggcatttc agcggctgaa
    74041 tttcagagca caggatccac accactggcg cggcgctcat cgcataaccg gcaatacaca
    74101 tcatcgtcat gccaacagag agccaggaca agccactgga agccgtaccg ttatcaaact
    74161 gcatcaggca atagcccagc accagagtgc ctaacgccat cacgctgaaa ccaattttca
    74221 gagccggttt acgacctgct ttatctaccg taaagaccgc aatgaaggtc gcgaacataa
    74281 aggtcagtcc cacgaccaga gtcgcaatca tctgttgttc tgtggtcgta aagcccgcca
    74341 ttttgaagat acgcggcgca tagtacatga tgatgttcat accggtaaac tgctgcatcg
    74401 cctgcaacaa cataccgaga aacacagcac gacggacgtt acggttgatc ttaaacagtg
    74461 cccaaccgcc ttgttttaac ttcaggcttt cacgaatttc gttgagttct tctcgcgctt
    74521 tttccgacgt atcgcgcagc atacgcaaca cttcttccgc ctcaatatga cgcccctttt
    74581 ccgccagcca gcgcgggcta tttggcagga agaccaccag aataatcagc aggactgctg
    74641 gtaaagcaag aacccccaac attgcgcgcc agttaccgct ataactgaac gctgtatcgg
    74701 ataaaaacgc cagcacgatg ccgagtgtga ccatcaactg gtacatactg atcatcttac
    74761 cgcgaacgtt ttcacttgcc atttcagaaa gatacagagg agcggtgtaa gacgcgatcc
    74821 cgacagcaat gcccagcacc acacgagcgg cgattaacat ctctacgctg gtcgcaaaag
    74881 cggaccctat agaaccgagt acaaacagga tggcccccgc catcaggctg tatttacgac
    74941 ccaggcggaa cgacagccaa ccattaaaca gcgcaccaat tgctgcaccg agcatcatgc
    75001 tactgaccac ccattcctgc aaacggctgg tcagcacaaa gtgatcggta atgaacggca
    75061 acgctccggc gattacgccg atatcaagac caaataacaa tcctgcgacc gcagcagcta
    75121 ccgaaacaaa catattcata cgccgcgtat cccgcaaaga acgtggcgtt aaagcagatt
    75181 ccgtattgat agtaaccatt ttttcctgcc agcagagagt aagacatagt gaaaaaatac
    75241 gtgaacaatt cacacaggtg tcaggtcgga aacagcataa atatggatta aattgctgcg
    75301 gcatgtcgtt atgtgatgga tattccaatt ttc
//
LOCUS       DAZGVQ010000004        62744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000004 DAZGVQ010000000
VERSION     DAZGVQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.004"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            231..1178
                     /gene="cyoA"
                     /locus_tag="ACHLAG_000234"
     CDS             231..1178
                     /gene="cyoA"
                     /locus_tag="ACHLAG_000234"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFP0653544.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            1200..3191
                     /gene="cyoB"
                     /locus_tag="ACHLAG_000235"
     CDS             1200..3191
                     /gene="cyoB"
                     /locus_tag="ACHLAG_000235"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFP0653545.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            3181..3795
                     /gene="cyoC"
                     /locus_tag="ACHLAG_000236"
     CDS             3181..3795
                     /gene="cyoC"
                     /locus_tag="ACHLAG_000236"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFP0653546.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3795..4124
                     /gene="cyoD"
                     /locus_tag="ACHLAG_000237"
     CDS             3795..4124
                     /gene="cyoD"
                     /locus_tag="ACHLAG_000237"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFP0653547.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            4136..5026
                     /gene="cyoE"
                     /locus_tag="ACHLAG_000238"
     CDS             4136..5026
                     /gene="cyoE"
                     /locus_tag="ACHLAG_000238"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFP0653548.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            5211..5522
                     /locus_tag="ACHLAG_000239"
     CDS             5211..5522
                     /locus_tag="ACHLAG_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFP0653549.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            5506..6048
                     /locus_tag="ACHLAG_000240"
     CDS             5506..6048
                     /locus_tag="ACHLAG_000240"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0653550.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(6104..7039)
                     /locus_tag="ACHLAG_000241"
     CDS             complement(6104..7039)
                     /locus_tag="ACHLAG_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP0653551.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRLGIMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPENFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            7447..8811
                     /gene="yajR"
                     /locus_tag="ACHLAG_000242"
     CDS             7447..8811
                     /gene="yajR"
                     /locus_tag="ACHLAG_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0653552.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(8939..9430)
                     /gene="yajQ"
                     /locus_tag="ACHLAG_000243"
     CDS             complement(8939..9430)
                     /gene="yajQ"
                     /locus_tag="ACHLAG_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFP0653553.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            9598..10509
                     /gene="panE"
                     /locus_tag="ACHLAG_000244"
     CDS             9598..10509
                     /gene="panE"
                     /locus_tag="ACHLAG_000244"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP0653554.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            10472..11062
                     /gene="yajL"
                     /locus_tag="ACHLAG_000245"
     CDS             10472..11062
                     /gene="yajL"
                     /locus_tag="ACHLAG_000245"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFP0653555.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(11116..12564)
                     /gene="thiI"
                     /locus_tag="ACHLAG_000246"
     CDS             complement(11116..12564)
                     /gene="thiI"
                     /locus_tag="ACHLAG_000246"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFP0653556.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            12770..13012
                     /gene="xseB"
                     /locus_tag="ACHLAG_000247"
     CDS             12770..13012
                     /gene="xseB"
                     /locus_tag="ACHLAG_000247"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFP0653557.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            13012..13911
                     /gene="ispA"
                     /locus_tag="ACHLAG_000248"
     CDS             13012..13911
                     /gene="ispA"
                     /locus_tag="ACHLAG_000248"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFP0653558.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            13936..15798
                     /gene="dxs"
                     /locus_tag="ACHLAG_000249"
     CDS             13936..15798
                     /gene="dxs"
                     /locus_tag="ACHLAG_000249"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFP0653559.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSALRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            15978..16952
                     /gene="yajO"
                     /locus_tag="ACHLAG_000250"
     CDS             15978..16952
                     /gene="yajO"
                     /locus_tag="ACHLAG_000250"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFP0653560.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGRNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(17006..17524)
                     /gene="pgpA"
                     /locus_tag="ACHLAG_000251"
     CDS             complement(17006..17524)
                     /gene="pgpA"
                     /locus_tag="ACHLAG_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFP0653561.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(17502..18479)
                     /gene="thiL"
                     /locus_tag="ACHLAG_000252"
     CDS             complement(17502..18479)
                     /gene="thiL"
                     /locus_tag="ACHLAG_000252"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFP0653562.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(18557..18976)
                     /gene="nusB"
                     /locus_tag="ACHLAG_000253"
     CDS             complement(18557..18976)
                     /gene="nusB"
                     /locus_tag="ACHLAG_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFP0653563.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(18996..19466)
                     /gene="ribE"
                     /locus_tag="ACHLAG_000254"
     CDS             complement(18996..19466)
                     /gene="ribE"
                     /locus_tag="ACHLAG_000254"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFP0653564.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(19555..20658)
                     /gene="ribD"
                     /locus_tag="ACHLAG_000255"
     CDS             complement(19555..20658)
                     /gene="ribD"
                     /locus_tag="ACHLAG_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFP0653565.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(20662..21111)
                     /gene="nrdR"
                     /locus_tag="ACHLAG_000256"
     CDS             complement(20662..21111)
                     /gene="nrdR"
                     /locus_tag="ACHLAG_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFP0653566.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            21262..21801
                     /gene="yajI"
                     /locus_tag="ACHLAG_000257"
     CDS             21262..21801
                     /gene="yajI"
                     /locus_tag="ACHLAG_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP0653567.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            22100..22984
                     /gene="tsx"
                     /locus_tag="ACHLAG_000258"
     CDS             22100..22984
                     /gene="tsx"
                     /locus_tag="ACHLAG_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFP0653568.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(23161..23508)
                     /gene="yajD"
                     /locus_tag="ACHLAG_000259"
     CDS             complement(23161..23508)
                     /gene="yajD"
                     /locus_tag="ACHLAG_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFP0653569.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(23637..24608)
                     /gene="secF"
                     /locus_tag="ACHLAG_000260"
     CDS             complement(23637..24608)
                     /gene="secF"
                     /locus_tag="ACHLAG_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFP0653570.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(24619..26466)
                     /gene="secD"
                     /locus_tag="ACHLAG_000261"
     CDS             complement(24619..26466)
                     /gene="secD"
                     /locus_tag="ACHLAG_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFP0653571.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(26494..26826)
                     /gene="yajC"
                     /locus_tag="ACHLAG_000262"
     CDS             complement(26494..26826)
                     /gene="yajC"
                     /locus_tag="ACHLAG_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFP0653572.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(26849..27976)
                     /gene="tgt"
                     /locus_tag="ACHLAG_000263"
     CDS             complement(26849..27976)
                     /gene="tgt"
                     /locus_tag="ACHLAG_000263"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFP0653573.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(28032..29102)
                     /gene="queA"
                     /locus_tag="ACHLAG_000264"
     CDS             complement(28032..29102)
                     /gene="queA"
                     /locus_tag="ACHLAG_000264"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFP0653574.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            29195..29776
                     /gene="acpH"
                     /locus_tag="ACHLAG_000265"
     CDS             29195..29776
                     /gene="acpH"
                     /locus_tag="ACHLAG_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFP0653575.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(29781..31598)
                     /gene="malZ"
                     /locus_tag="ACHLAG_000266"
     CDS             complement(29781..31598)
                     /gene="malZ"
                     /locus_tag="ACHLAG_000266"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFP0653576.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYHSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVAQWQRKEGHGQLTNGILALPAISATVWMN"
     gene            complement(31754..33127)
                     /gene="proY"
                     /locus_tag="ACHLAG_000267"
     CDS             complement(31754..33127)
                     /gene="proY"
                     /locus_tag="ACHLAG_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFP0653577.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(33203..34522)
                     /gene="brnQ"
                     /locus_tag="ACHLAG_000268"
     CDS             complement(33203..34522)
                     /gene="brnQ"
                     /locus_tag="ACHLAG_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFP0653578.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(34929..36224)
                     /gene="phoR"
                     /locus_tag="ACHLAG_000269"
     CDS             complement(34929..36224)
                     /gene="phoR"
                     /locus_tag="ACHLAG_000269"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFP0653579.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRLLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(36282..36971)
                     /gene="phoB"
                     /locus_tag="ACHLAG_000270"
     CDS             complement(36282..36971)
                     /gene="phoB"
                     /locus_tag="ACHLAG_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFP0653580.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            37161..38363
                     /gene="sbcD"
                     /locus_tag="ACHLAG_000271"
     CDS             37161..38363
                     /gene="sbcD"
                     /locus_tag="ACHLAG_000271"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFP0653581.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQQYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            38360..41503
                     /gene="sbcC"
                     /locus_tag="ACHLAG_000272"
     CDS             38360..41503
                     /gene="sbcC"
                     /locus_tag="ACHLAG_000272"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFP0653582.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLIDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     ANQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            41629..42813
                     /gene="araJ"
                     /locus_tag="ACHLAG_000273"
     CDS             41629..42813
                     /gene="araJ"
                     /locus_tag="ACHLAG_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFP0653583.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(42956..43864)
                     /gene="mak"
                     /locus_tag="ACHLAG_000274"
     CDS             complement(42956..43864)
                     /gene="mak"
                     /locus_tag="ACHLAG_000274"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFP0653584.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            43989..44900
                     /gene="rdgC"
                     /locus_tag="ACHLAG_000275"
     CDS             43989..44900
                     /gene="rdgC"
                     /locus_tag="ACHLAG_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFP0653585.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(44978..45061)
                     /locus_tag="ACHLAG_000276"
     CDS             complement(44978..45061)
                     /locus_tag="ACHLAG_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0653586.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(45058..45399)
                     /locus_tag="ACHLAG_000277"
                     /pseudo
     CDS             complement(45058..45399)
                     /locus_tag="ACHLAG_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(45547..45831)
                     /gene="ppnP"
                     /locus_tag="ACHLAG_000278"
     CDS             complement(45547..45831)
                     /gene="ppnP"
                     /locus_tag="ACHLAG_000278"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFP0653587.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(45903..46580)
                     /gene="aroM"
                     /locus_tag="ACHLAG_000279"
     CDS             complement(45903..46580)
                     /gene="aroM"
                     /locus_tag="ACHLAG_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFP0653588.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(46838..47029)
                     /gene="yaiA"
                     /locus_tag="ACHLAG_000280"
     CDS             complement(46838..47029)
                     /gene="yaiA"
                     /locus_tag="ACHLAG_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFP0653589.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPESLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(47079..47603)
                     /gene="aroL"
                     /locus_tag="ACHLAG_000281"
     CDS             complement(47079..47603)
                     /gene="aroL"
                     /locus_tag="ACHLAG_000281"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFP0653590.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(47786..48244)
                     /gene="yaiI"
                     /locus_tag="ACHLAG_000282"
     CDS             complement(47786..48244)
                     /gene="yaiI"
                     /locus_tag="ACHLAG_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFP0653591.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            48364..49173
                     /gene="proC"
                     /locus_tag="ACHLAG_000283"
     CDS             48364..49173
                     /gene="proC"
                     /locus_tag="ACHLAG_000283"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFP0653592.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(49190..50305)
                     /gene="adrA"
                     /locus_tag="ACHLAG_000284"
                     /gene_synonym="dgcC"
     CDS             complement(49190..50305)
                     /gene="adrA"
                     /locus_tag="ACHLAG_000284"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFP0653593.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(50407..50727)
                     /gene="psiF"
                     /locus_tag="ACHLAG_000285"
     CDS             complement(50407..50727)
                     /gene="psiF"
                     /locus_tag="ACHLAG_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFP0653594.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(50846..52261)
                     /gene="phoA"
                     /locus_tag="ACHLAG_000286"
     CDS             complement(50846..52261)
                     /gene="phoA"
                     /locus_tag="ACHLAG_000286"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFP0653595.1"
                     /translation="MKQSTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(52362..52622)
                     /gene="iraP"
                     /locus_tag="ACHLAG_000287"
     CDS             complement(52362..52622)
                     /gene="iraP"
                     /locus_tag="ACHLAG_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFP0653596.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(52804..53007)
                     /locus_tag="ACHLAG_000288"
     CDS             complement(52804..53007)
                     /locus_tag="ACHLAG_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0653597.1"
                     /translation="MLQSSADEACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            53085..54179
                     /gene="ddlA"
                     /locus_tag="ACHLAG_000289"
     CDS             53085..54179
                     /gene="ddlA"
                     /locus_tag="ACHLAG_000289"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP0653598.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLLTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(54203..54415)
                     /gene="yaiZ"
                     /locus_tag="ACHLAG_000290"
     CDS             complement(54203..54415)
                     /gene="yaiZ"
                     /locus_tag="ACHLAG_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFP0653599.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            54675..54983
                     /gene="yaiY"
                     /locus_tag="ACHLAG_000291"
     CDS             54675..54983
                     /gene="yaiY"
                     /locus_tag="ACHLAG_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFP0653600.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(55042..56136)
                     /gene="yaiW"
                     /locus_tag="ACHLAG_000292"
     CDS             complement(55042..56136)
                     /gene="yaiW"
                     /locus_tag="ACHLAG_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFP0653601.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(56149..57369)
                     /gene="sbmA"
                     /locus_tag="ACHLAG_000293"
     CDS             complement(56149..57369)
                     /gene="sbmA"
                     /locus_tag="ACHLAG_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFP0653602.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDSVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            57721..58878
                     /gene="ampH"
                     /locus_tag="ACHLAG_000294"
     CDS             57721..58878
                     /gene="ampH"
                     /locus_tag="ACHLAG_000294"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFP0653603.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(58879..59502)
                     /gene="iprA"
                     /locus_tag="ACHLAG_000295"
     CDS             complement(58879..59502)
                     /gene="iprA"
                     /locus_tag="ACHLAG_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFP0653604.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(59590..62568)
                     /gene="ehaB"
                     /locus_tag="ACHLAG_000296"
     CDS             complement(59590..62568)
                     /gene="ehaB"
                     /locus_tag="ACHLAG_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFP0653605.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCAERANDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVDVYNIGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWSDEGTSGWFGNTGNASDYNGGDWNRDDIALAVIAHPAAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPHGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHLTVDTNDDGVPDAYGPVYNDNGVVIDTSTGTEATATLIDIAANS
                     LWPSSTVGVENSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINS
                     GSTVNVADSTLISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGD
                     AALYANNIKVTNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLD
                     ADLTNDRTNDTTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVA
                     AATGNYKVRIDNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRG
                     NTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFG
                     GNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDS
                     QTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYD
                     FKLGDAGYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSED
                     QALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANY
                     LGGGDVDQDWSANVGVKYTW"
BASE COUNT        15499 a        16436 c        15616 g        15193 t
ORIGIN      
        1 cagattggtt ataccaattg cccgtccaaa ctccttctgc actccccttt cgttataacg
       61 cccttttgca acagcttctt aaaatcaact tgatatgttt tgcaacatat gtgacctgac
      121 agccaaatcc aagtaacagg aatttaatca tgtttacagt aatgtaacct tcccgtaaaa
      181 tgcccacaca ctttaaacgc caccagatcc cgtggaattg aggtcgttaa atgagactca
      241 ggaaatacaa taaaagtttg ggatggttgt cattatttgc aggcactgta ttgctcagtg
      301 gctgtaattc tgcgctgtta gatcccaaag gacagattgg tctggagcaa cgttcactga
      361 tactgacggc atttggcctg atgttgattg tcgttattcc cgcaatcttg atggctgttg
      421 gtttcgcctg gaagtatcgt gcgagcaata aagatgctaa gtacagcccg aactggtcac
      481 actccaataa agtggaagct gtggtctgga cggtacctat cttaatcatc atcttccttg
      541 cggtactgac ctggaaaacc actcacgctc ttgagcctag caagccgctg gcacacgacg
      601 agaagcccat taccatcgaa gtggtttcca tggactggaa atggttcttc atctacccgg
      661 aacagggcat tgctaccgtg aatgaaatcg ctttcccggc gaacactccg gtgtacttca
      721 aagtgacctc caactccgtg atgaactcct tcttcattcc gcgtctgggt agccagattt
      781 atgccatggc cggtatgcag actcgcctgc atctgatcgc caacgaaccc ggtacttatg
      841 acggtatctc cgccagctac agcgggccgg gcttctcagg catgaagttc aaagctattg
      901 caacaccgga tcgcgccgaa ttcgaccaat gggtcgcaaa agcgaaacag tcgccgaaca
      961 ccatgtctga catggctgcg ttcgaaaaac tggccgcgcc tagcgaatac aaccaggtgg
     1021 aatatttctc caacgtgaaa ccagacttgt ttgccgatgt gattaacaag tttatggctc
     1081 acggtaagag catggacatg acccagccag aaggtgagca cagtgcacac gaaggtatgg
     1141 aaggcatgga catgagccac gcggaatccg cccattaaag gggttgagga agaataaaga
     1201 tgttcggaaa attatcactt gatgcagtcc cgttccatga acctatcgtc atggttacga
     1261 tcgctggcat tattttggga ggtctggcgc tcgttggcct gatcacttac ttcggtaagt
     1321 ggacctacct gtggaaagag tggctgacct ccgtcgacca taaacgcctc ggtatcatgt
     1381 atatcatcgt ggcgattgtg atgttgctgc gtggttttgc tgacgccatt atgatgcgta
     1441 gccagcaggc tcttgcctcg gcgggcgaag cgggcttcct gccacctcac cactacgatc
     1501 agatcttcac cgcgcacggc gtgattatga tcttcttcgt ggcgatgcct ttcgttatcg
     1561 gtctgatgaa cctggtggtt ccgctgcaga tcggcgcgcg tgacgttgcg ttcccgttcc
     1621 tcaacaactt aagcttctgg tttactgttg ttggtgtgat tctggttaac gtttctctcg
     1681 gcgtgggcga atttgcgcag accggctggc tggcctatcc accgctatcg ggaatagagt
     1741 acagtccggg agtcggtgtc gattactgga tatggagtct ccagctatcc ggtataggta
     1801 cgacgcttac cggtatcaac ttcttcgtta ccattctgaa gatgcgcgca ccgggcatga
     1861 ccatgttcaa gatgccagta tttacctggg catcactgtg cgcgaacgta ctgattattg
     1921 cttccttccc aattctgacg gttaccgtcg cgttgttgac cctggatcgc tatctgggca
     1981 cccatttctt taccaacgat atgggtggca acatgatgat gtacatcaac ctgatttggg
     2041 cctggggcca cccggaagtt tacatcctga tcctgcctgt tttcggtgtg ttctccgaaa
     2101 ttgcggcaac cttctcgcgt aaacgtctgt ttggttatac ctcgctggta tgggcaaccg
     2161 tctgtatcac cgtgctgtcg ttcatcgttt ggctgcacca cttctttacg atgggtgcgg
     2221 gcgcgaacgt aaacgccttc tttggtatca ccacaatgat tatcgccatc ccgaccgggg
     2281 tgaagatctt caactggctg ttcaccatgt atcagggccg catcgtgttc cattctgcga
     2341 tgctgtggac catcggtttt atcgtcactt tctcggtggg cgggatgact ggcgtgctgc
     2401 tggcagtacc gggcgcggac ttcgttctgc ataacagcct gttcctgatt gcgcacttcc
     2461 ataacgtgat catcggcggc gtggtcttcg gctgcttcgc agggatgacc tactggtggc
     2521 ctaaagcctt cggtttcaaa ctgaatgaaa cctggggtaa acgcgcgttc tggttctgga
     2581 tcatcggctt cttcgttgcc tttatgccgc tgtatgcgct gggcttcatg ggcatgaccc
     2641 gtcgtttgag ccagcagatt gacccgcagt tccacaccat gctgatgatt gcagctagcg
     2701 gtgcagtact gattgcgctg ggtattctct gcctcgttat tcagatgtac gtttctattc
     2761 gcgaccgcga ccagaaccgt gacctgactg gcgacccgtg gggtggccgt acgctggagt
     2821 gggcaacctc ttccccgcct ccgttctata actttgccgt tgtgccgcac gttcacgagc
     2881 gtgatgcatt ctgggaaatg aaagagaaag gcgaagcgta taaaaagcct gaccactatg
     2941 aagaaattca tatgccgaaa aacagcggtg caggtatcgt cattgcagct ttctccacca
     3001 tcttcggttt cgccatgatc tggcatatct ggtggctggc gattgttggc ttcgcaggca
     3061 tgatcatcac ctggatcgtg aaaagcttcg acgaggacgt ggattactac gtgccggtgg
     3121 cagaaatcga aaaactggaa aaccagcatt tcgatgagat tactaaggca gggctgaaaa
     3181 atggcaactg atactttgac gcacgcgact gcccacgcgc acgaacacgg gcaccacgat
     3241 gcaggcggaa ccaaaatctt cggattttgg atctacctga tgagcgactg cattctgttc
     3301 tcaatcttgt ttgctaccta tgccgttctg gtgaacggca ccgcaggcgg cccgacaggt
     3361 aaggacattt tcgaactgcc gttcgttctg gttgaaactt tcttgctgtt gttcagctcc
     3421 atcacctacg gcatggcggc tatcgccatg tacaaaaaca acaaaagcca ggttatctcc
     3481 tggctggcgt tgacctggtt gtttggtgcc ggatttatcg ggatggaaat ctacgaattc
     3541 catcacctga ttgttaacgg catgggtccg gatcgcagcg gcttcctgtc agcgttcttt
     3601 gcgctggtcg gcacgcacgg tctgcacgtc acttccggtc ttatctggat ggcggtgctg
     3661 atggtacaaa tcgctcgtcg cggcctgacc agcactaacc gtacccgcat catgtgcctg
     3721 agcctgttct ggcacttcct ggatgtggtt tggatctgtg tgttcactgt tgtttatctg
     3781 atgggggcga tgtaatgagt cattctaccg atcacagcgg cgcgtcccat ggcagcgtaa
     3841 aaacctacat gacaggcttt atcctgtcga tcattctgac ggtgattccg ttctggatgg
     3901 tgatgacagg ggctgcctct ccggccgtaa ttctgggaac aatcctggca atggcagtgg
     3961 tacaggttct ggtgcatctg gtgtgcttcc tgcacatgaa taccaaatca gatgaaggct
     4021 ggaacatgac ggcgtttgtc ttcaccgtgc taatcatcgc tatcctggtt gtaggctcca
     4081 tctggattat gtggaacctc aactacaaca tgatgatgca ctaagagcgg cggttatgat
     4141 gtttaagcaa tacctgcaag taacgaaacc aggcatcatc tttggcaacc tgatctcggt
     4201 gattggggga ttcctgctgg cctcaaaggg cagcattgat tatcccctgt ttatctacac
     4261 gctggttggg gtgtcactgg ttgtggcgtc gggttgtgtg tttaacaact acatcgacag
     4321 ggatatcgac agaaagatgg aaaggacgaa gaatcgggtg ctggttaaag gcctgatctc
     4381 tcctgctgtc tcgctggtgt acgccacctt gctgggtatt gctggcttta tgctgctgtg
     4441 gtttggcgcg aatccgctgg cctgctggct gggggtgatg ggctttgtgg tttatgtcgg
     4501 cgtctatagc ctgtacatga aacgccactc agtctacggc acattgattg gttcgctctc
     4561 cggcgctgcg ccgccggtga ttggctactg tgcagtaacc ggcgagttcg atagcggcgc
     4621 agcgatcctg ctggctatct tcagcctgtg gcagatgcct cactcctatg ccatcgccat
     4681 tttccgcttt aaggattacc aggcggcaaa cattccggta ttgccagtgg taaaaggcat
     4741 ttcggtggcg aagaatcaca tcacgctgta tatcatcgcc tttgccgttg ccacgctgat
     4801 gctctctctt ggcggttacg ctggatataa atatctggtg gtcgccgcgg cggttagcgt
     4861 ctggtggtta ggtatggctc tgcgcggtta taaagttgct gatgacagaa tctgggcgcg
     4921 caagctgttc ggcttctcta tcatcgccat cactgccctc tcggtgatga tgtccgttga
     4981 ttttatggta ccggactcgc atacgctgct ggctgctgtg tggtaacaaa acctctctat
     5041 taaaaaggtg ctacggcacc ttttttattg tctgagatca gagatacggc taataaccgt
     5101 atgtcttgac agagtgagca gatgaacata aattaatcat acggttgata gccgtattat
     5161 gtatgagatc gactccctac gatttcattc agttaatcaa agaggtcata atgcctggaa
     5221 aaacagccac tctacccgat gtcgataaaa cgctgaaaaa tgcgcgggtc gaactcaaaa
     5281 ctagtcccga cgccaaaaac aaacttcgtg aagctgcgca agccgttggg gtagatttaa
     5341 gcgcttttat tttgagcgct gctatggaac gcgccgaaag tgtgcttgat aaccaacgtc
     5401 gccgtgagct ttcgaatcaa agctgggaac tgatgaacca actcatcgct gaacctgctc
     5461 aaccgacgct cgccctcaag gcgttaatga aaaggaaaaa cagcgatggt cgacaagcat
     5521 gaagagatta ctctgcccat agtcctctcc tgtaattatc agtctgatat tacttatcct
     5581 gggcaaaaac agtttgattg cggtaaccct gttatcgata aatttgtacg cgcatcgcta
     5641 aagaaaagtg tgcgtaatag cgactgtgcg gctaaagcac ttattgacag acaaagtggt
     5701 gaactgatcg gcatctgtac ttttacggca tattcgctgg aaaaacaacg cgtttctggc
     5761 gtccttcagg gttcacaacc ttcagaaatt ggtgttgtca gattagtcat gttgggggta
     5821 gcacggaagt atcaaaagcg gggctttggt caggacctac tatgtgattt ttttgaacat
     5881 gtaaaaataa ttcaccaggc attaccaatt aaaggggttt atcttgatgc tgaccctgcc
     5941 gccattaatt tttatgctcg tctcggcttt gttcagcttt cagcgacacc aaatgctttt
     6001 ggtgctgtac ctatgttttt ggcgattcag catattctcg cggcttagat tattatcgcc
     6061 acccgccagg gcaagttttg cctcagccgg gggctgagca gcgttaccac ttttgttcaa
     6121 ttgcccccgc tttcagcaac tccacatcct tgccgttaat ggttgccgtt gcggcaacga
     6181 ttgtggtcct tacagttgca caagattttc gctcaaattc cggtttataa agaataagag
     6241 acgaattact ggtaaatgaa ccaaataggt aatcagtaaa gctaatattc agccggttta
     6301 aaccgaaagg cgcgaagcca accgtttggt taatctcctt accggatggg cgacgaatgc
     6361 tgaatgtcac gcgaagattg gccacttcgc ctaatttatt ggtggtggcg aagaggctat
     6421 ccatgcagga aaaaccttta tctaaaaaat tatctcgcat cccagaacgg gtaaccgtaa
     6481 tcacgggtaa cgtatcgtca accgtttttc tgtcaatcat gtcttttcga caagtagcca
     6541 gaaagttttc tgggccacca taaatgtgga cacctttatc aagatattcc ttcgctttct
     6601 tacaatctac ctcaacatac tgaccattgg cgtacataat gcccaacctg gaccatgcta
     6661 aattcacacc gtgctgcgcg gcctgattaa atagtgcata cgctttggca taatccaccc
     6721 gcacctgctc accgtcgtag tagatacccc ccagcgctaa cggagcttct ctgtcacctt
     6781 gtttagccgc taactcgaac catttaatcg cttcctgaac atcaggtttg gtatcataac
     6841 gaggctgata atattcccag cctaacaggc tctgcgcgtc gatatctccc gattcagcca
     6901 ttttatgcag tgtatttata acatcgggag caggtttacc accgtatatg ctgtcataca
     6961 attcatgcgc tttctcgctg gccaccacgc ttggtgaaac tatcagcatt acagaaaata
     7021 atattctttt agttaacatg tataccttta tgtcctcact gtcatatatt tccataataa
     7081 ttaaccacca aacatccatt taatgaataa atacagtgcc ataaaggtcc aagtaataag
     7141 cacacattta gcctgaaaca ttatcatcca tgtcaccaaa tcgtttattt agtgagataa
     7201 aactagcata ttcgtaagat aaaaaatgca catagcaatc aagaaatgat attattactt
     7261 ttttgatgta aaactattta gttttaaaat tcattttttc aacatataga gcattcattt
     7321 tatgaatgca aaaaaacata cctgacacac aaattactct cctctgtaat attttctcat
     7381 taacactctg tttacccaac atgcgctccg cactacacta tgtgggcatt tttttaaggg
     7441 gtggtaatga acgattataa aatgacgcca ggtgagaggc gcgcgacctg gggtttaggg
     7501 accgtattct cgttgcgcat gctgggcatg tttatggttc tgccggttct gaccacgtac
     7561 ggcatggctc tgcaaggtgc cagcgaagca ttaatcggta ttgccattgg tatttatggt
     7621 ctgactcagg ccgtttttca gattccgttt ggcctgcttt ccgaccgcat tggtcgcaaa
     7681 ccattaattg tcggtgggct ggcggtgttt gccgccggta gcgttatcgc tgcgctttcc
     7741 gactccatct ggggaattat tctgggccgg gcgctacaag gctctggagc aattgccgcc
     7801 gccgttatgg cgctgctttc cgatctcacg cgcgaacaaa accgcaccaa agcgatggcg
     7861 tttatcggcg tgagctttgg cattaccttt gccattgcga tggtgcttgg cccgatcatc
     7921 actcacaaac ttgggctgca cgcgctgttc tggatgatcg ctattctggc aacaaccggc
     7981 attgcgttga ccatttgggt tgtgcccaac agtagcactc acgtacttaa tcgtgagtcc
     8041 ggaatggtga aaggcagttt cagtaaagtg ctggcggaac cgcggctgct aaaactcaac
     8101 tttggcatta tgtgtctgca catgcttctg atgtctacgt ttgttgccct gcccggacag
     8161 ctagctgatg cggggttccc ggcggctgaa cactggaagg tctatctggc gacgatgtta
     8221 atcgcctttg gctcggtcgt gcctttcatt atctacgctg aagttaagcg caaaatgaag
     8281 caagtctttg tcttctgcgt cgggttgatc gtggttgcgg aaattgtgtt gtggaacgca
     8341 caaacgcagt tctggcaact ggtggtcggc gtgcagcttt tctttgtggc gtttaatttg
     8401 atggaagccc ttctgccctc acttatcagt aaagagtcgc cagcaggtta caaaggtacg
     8461 gcgatgggtg tctactccac cagccagttt cttggcgtgg cgattggcgg ttcgctgggc
     8521 ggctggattg acggcatgtt tgacggtcag ggggtatttc tcgctggcgc aatgctggcc
     8581 gcagtgtggc tggcagtcgc cagtaccatg aaagaaccgc cgtatgtcag cagtttgcgc
     8641 attgaaatcc cggcggacat tgccgcaaac gaagcgttaa aagtgcgttt gctggaaact
     8701 gaaggcatca aagaagtgtt gattgcagaa gaagaacatt cagcttatgt gaaaatcgac
     8761 agcaaagtga cgaatcgctt tgagatagaa caggcaattc gtcaggcata aaaaacgcgg
     8821 cgaattgcct gctgcgctac gcttatcagg cctacaaagt ttctgaatta caaacctttg
     8881 taggccgaat caggcgttca tgccgcatct gacatgaaca aaacgcacat agtcgcgatt
     8941 aatcgcggaa gtttttgaac tggaacggct gaccgagatc gccaccacgt accatcgcca
     9001 tgacagcctg caaatcatca cgagatttgc ccgttacgcg gatttcatcg ccctgaattt
     9061 gcgcctgcac tttcagtttg ctgtctttga tcatcttgac gattttcttc tgggtcgcgc
     9121 tctcaatgcc ctgtttcagt ttcgcttcca caaaccaggt tttaccgcta tgaacgatat
     9181 tctccggtac atccagcgaa ctgccttcaa tgccgcgctt cagcagcttg gcacgcagaa
     9241 tatccagcaa ctgattgacc tggaaatcgg actcgctcaa cactttgatg gttttgctgg
     9301 cgtcgttcag ctcaaatgag gcttcaacgt tacggaagtc aaaacgggac tccacttcgc
     9361 ggctcgcgtt atcgaccgcg ttacgtgctt cctgaagatc aacttcagag acaatatcga
     9421 aagatggcat cttttcctct cccttcattt ttgatgcgaa gcataatacc cgcaaagtta
     9481 aataaccgac agtcctgcac caggacgtat cccttaattg tgcatagtcg caacagcatg
     9541 cacaattaag ggatagcctt attgaacacc cggagtggtt gcgggtgagg aggaacaatg
     9601 aaaattaccg tattgggatg cggtgcctta gggcaattat ggcttacagc actttgcaaa
     9661 cagggtcatg aagttcaggg ctggctgcgc gtaccgcaac cttattgtag cgtgaatctg
     9721 gttgagacag atggttcgat atttaacgaa tcgctgaccg ccaacgatcc cgattttctt
     9781 gccaccagcg atctgctcct ggtgacgctg aaagcatggc aggtttacga tgccgtcaaa
     9841 agcctcgcgt ccacactgcc tgtaaccacg ccaatactgt taattcacaa cggcatgggc
     9901 accatcgaag agttgcaaaa cattcagcag ccattactga tgggcaccac cacccatgcc
     9961 gcccgccgcg acggcaatgt cattattcat gtggcaaacg gaatcacgca tattggtccg
    10021 gcacggcaac aggatggcga ttacagttat ctggcggata ttttgcaaac cgtgttgcct
    10081 gacgttgcct ggcataacaa tattcgcgcc gagctgtggc gcaagctggc cgtcaactgt
    10141 gtgattaatc cactgaccgc catctggaat tgcccgaatg gtgaattacg tcatcatccg
    10201 caagaaatta tgcagatatg cgaagaagtc gcggcagtga tcgagcgcga agggcatcat
    10261 acttcagcag aagatttgcg tgattacgtg atgcaggtga ttgatgccac agcggaaaat
    10321 atctcgtcga tgttgcagga tatccgcgcg ctgcgccaca ccgaaatcga ctatatcaat
    10381 ggttttctct tacgccgcgc ccgcgcgcat gggattgccg taccggaaaa cacccgcctg
    10441 tttgaaatgg taaaaagaaa ggagagtgaa tatgagcgca tcggcactgg tttgcctcgc
    10501 ccctggtagt gaagagactg aagccgtcac cactatcgat ctgctggttc gcggcggtat
    10561 caaagtcacc actgccagcg tcgccagcga tggcaacctg gccattacct gctcgcgcgg
    10621 cgtgaagctg ctggcggatg cgccgctggt cgaggtggct gatggcgaat atgacgtgat
    10681 cgtactgcct ggtggcatta aaggcgcaga gtgttttcgc gatagtaccc tgctggttga
    10741 aaccgttaaa cagttccacc gttccgggcg tatcgtcgcg gctatttgcg ccgcgccagc
    10801 caccgtgctg gtgccgcacg atatcttccc gattggtaat atgaccggct tcccgacgct
    10861 gaaagacaaa attcccgctg aacaatggca ggacaagcgc gtcgtctggg atgcacgggt
    10921 aaaattgctg accagccagg ggccggggac agctatcgac tttggtctga aaattatcga
    10981 cctgctggtt gggcgcgaga aagcccatga agtggcatca caactggtga tggcggcagg
    11041 gatttataat tattacgagt agtgtcggat gcggtaagcg tcgcatctga ccagatgcga
    11101 cgttacaacc acaaattacg ggcgatatac cttcacattg ttaaagccct gctcgcgcag
    11161 atagagcgcc tgcagacggc tcatcacccc gcgctcacac cacagtagcc aggttctgtt
    11221 ctggtcgaga tcgccaaact tggtgctcag tttatagaac ggcagagaaa ctacatcaat
    11281 cccttcgact ttcagtggct tatcttcctg ttcatcgata gaacggatat cgaggatcac
    11341 gtcgttcggg ccgaagccat tgacggtttc cacttccacc acttcctgct cggtctgctg
    11401 ggcgatttcg cggatatcaa cgttattcgc ttcttcaacc actttatcga gaatgctgaa
    11461 gtcgaatttc tcttcttccg cttcaatctt cgacttaacc gctttcaccg tcgggctttt
    11521 ggagataaca ccgcaatatt ccggcatcgt gcgagcaaag tcttcggtgc caatctggcg
    11581 agccaggttg atgatgtgct ctttgtcgta agaaatcagc ggacgcagga tcagcgtatc
    11641 ggagacgtta tcaatcaggc gcaggttggt cagcgtctga ctggacacct ggccgagcgc
    11701 ttcgccggtg accagcgcct gtacgccgta acgttcagcc actttagacg cggcacgcac
    11761 catcatacgt ttgaggataa cgcccatctg accgtcgtcg attttctcga gaatttcccc
    11821 gacgaccggt tcgaaattaa tagcgacaaa acgcacgcgg tgggagctgc caaaacggtt
    11881 ccacagataa tgcgccacct gacgcacgcc aatttcatgc gccgcgccgc caaggttaaa
    11941 gaaacagtaa tgcacgcggc agccgcgacg catcaacata taactggaaa caccggagtc
    12001 gaaaccaccg gaaatgagcg acagcacatc ttcctgggtg ccgatcggga aaccgccaat
    12061 accttcgtag cggcctttaa tcagcaggag acgatcgtct tccacttcca gatggacagt
    12121 cacttccgga ttggtcagct tcacacgcgc ggactcaata tgctgattta aaccgccgcc
    12181 gacgtaacgt tccacatcaa tcgagctaaa atcatgtttg ccacggcgct tcacgcgtac
    12241 gcagaaggtt ttgccttcca gctgatcgcg atactgaacc aacgctttct caaaaatatc
    12301 gtgcatgtcg gtaaacggca cgtcttcgac ttcgagaata tggtggatac ccggaatacg
    12361 ggtcagggcg tcgcgaatag ccagacgctg gttttcatct tttgcgcgaa cttcgatgtt
    12421 atcccagtgg cggacgacag cgagcgtctc atcatagtgc tttaaaacgt tacgaatgtt
    12481 cccggtaagg atttttataa agcgcaagcg cacagattgg cttttgatgg tgatttccgg
    12541 gaacaattta atgataaact tcatggcggc aatggttcgt tggcaagcct taagcgactt
    12601 gtatagggaa aaatacagca gcccacacct gcggctgcat ccaggcgcgg aagtatacca
    12661 ctaacatcgc tttgctgcgc acatcacctt accattgcgc gttatttgct atttgccctg
    12721 agtccgttac catgacgggg cgaaaaatat tgagagtcag acattcatta tgccgaagaa
    12781 aaatgaggct cccgccagct ttgaaaaggc gctgagcgag ctggaacaga ttgtaacccg
    12841 tctggaaagt ggcgacctgc cgctggaaga ggcgctgaac gagttcgaac gcggcgtgca
    12901 gctggcacgt caggggcagg ccaaattaca acaagccgaa cagcgcgtac aaattctgct
    12961 gtctgacaat gaagacgcct ctctaacccc ttttacaccg gacaatgagt aatggacttt
    13021 ccgcagcaac tcgaagcctg cgttaagcag gccaaccagg cgctgagccg ttttatcgcc
    13081 ccactgccct ttcagaacac tcccgtggtc gaaaccatgc agtatggcgc attattaggt
    13141 ggtaagcgcc tgcgaccttt tctggtttat gccaccggtc atatgttcgg cgttagcaca
    13201 aacacgctgg acgcacccgc tgccgccgta gagtgtatcc acgcttactc attaattcat
    13261 gatgatttac cggcgatgga tgatgacgat ctgcgtcgcg gtttgccgac ctgccatgtg
    13321 aagtttggcg aagcaaacgc gattctcgct ggcgacgctt tacaaacgct ggcgttctcg
    13381 attctaagcg atgctgatat gccggaagtg tcggatcgcg acagaatttc gatgatttct
    13441 gaactggcga gcgccagcgg tattgccgga atgtgcggtg gtcaggcatt agatttagac
    13501 gcggaaggca aacacgtacc tctggacgcg cttgagcgta ttcatcgtca taaaaccggc
    13561 gcattgattc gcgccgccgt tcgccttggt gcattaagcg ccggagataa agggcgtcgt
    13621 gctctgccag tactcgacaa gtacgcagag agcatcggcc ttgccttcca ggttcaggat
    13681 gacatcctgg atgtggtagg agatactgca acgttgggaa aacgccaggg tgccgaccag
    13741 caacttggta aaagtaccta ccctgcactt ctgggtcttg agcaagcccg gaagaaagcc
    13801 cgggatctga tcgacgatgc ccgccagtcg ctgaaacaac tggctgaaca atcactcgat
    13861 acctcggcac tggaagcgct agcggactac atcatccagc gtaataaata aacaataagt
    13921 attaataggc ccctgatgag ttttgatatt gccaaatacc cgaccctggc actggtcgac
    13981 tccacccagg agttacgact gttgccgaaa gagagtttac cgaaactctg cgacgaactg
    14041 cgccgctatt tactcgacag cgtgagccgt tccagcgggc acttcgcctc cgggctgggc
    14101 acggtcgaac tgaccgtggc gctgcactat gtctacaaca ccccgtttga ccaattgatt
    14161 tgggatgtgg ggcatcaggc ttatccgcat aaaattttga ccggacgccg cgacaaaatc
    14221 ggcaccatcc gtcagaaagg cggcctgcac ccgttcccgt ggcgcggcga aagcgaatat
    14281 gacgtattaa gcgtcgggca ttcatcaacc tccatcagtg ccggaattgg tattgcggtt
    14341 gctgccgaga aagaaggcaa aaatcgccgc accgtctgtg tcattggcga tggcgcgatt
    14401 accgctggca tggcgtttga agcgatgaat cacgcgggcg atatccgtcc tgatatgctg
    14461 gtggtcctca acgacaatga aatgtcgatt tccgaaaatg tcggcgcgct caataaccat
    14521 ctggcacagc tgctttccgg taagctttac tcttcgctgc gcgaaggcgg gaaaaaagtt
    14581 ttctctggcg ttccgccaat taaagagctg ctcaaacgta ccgaagaaca tattaaaggc
    14641 atggtagtgc ctggcacgtt gtttgaagag ctgggcttta actacatcgg cccggttgac
    14701 ggtcacgatg tgctggggct tatcaccacg ctgaagaaca tgcgcgacct gaaaggcccg
    14761 cagttcctgc atatcatgac caaaaaaggt cgtggttatg aaccggcaga aaaagacccc
    14821 atcactttcc acgccgtgcc taaatttgat ccctccagcg gttgtttgcc gaaaagtagc
    14881 ggcggtttgc cgagctattc aaaaatcttt ggcgactggt tgtgcgaaac ggcagcgaaa
    14941 gacaacaagc tgatggcgat tactccggcg atgcgtgaag gttccggcat ggtcgagttt
    15001 tcacgtaaat tcccggatcg ttacttcgac gtggcaatcg ccgagcaaca cgcggtgacc
    15061 tttgccgccg gtctggcgat tggtgggtac aaacccattg tcgcgattta ctccactttc
    15121 ctgcaacgcg cctatgatca ggtgctgcat gacgtggcga ttcaaaagct cccggtcctg
    15181 ttcgccatcg accgcgcggg cattgttggt gctgacggtc aaacccatca gggcgctttt
    15241 gacctctctt acctgcgctg cataccggaa atggtcatta tgaccccgag cgatgaaaac
    15301 gaatgtcgcc agatgctcta taccggctat cactataacg atggcccgtc agcgttgcgc
    15361 tacccgcgtg gcaacgcggt cggcgtggaa ctgacgccgc tggaaaaact accaattggc
    15421 aaaggcattg tgaagcgtcg tggcgagaaa ctggcgatcc ttaactttgg tacgctgatg
    15481 ccagaagcgg cgaaagtcgc cgaatcgctg aacgccacgc tggtcgatat gcgttttgtg
    15541 aaaccgcttg atgaagcgtt aattctggaa atggccgcca gccatgaagc gctggtcacc
    15601 gtagaagaaa acgccattat gggcggcgca ggcagcggcg tgaacgaagt gctaatggcc
    15661 catcgtaaac cagtacccgt gctgaacatt ggcctgcctg acttctttat tccgcaagga
    15721 actcaggaag aaatgcgcgc cgaactcggc ctcgatgccg ccggtatgga agccaaaatc
    15781 aaggcctggc tggcataatc cctactccac tcctgctatg cttaacaaat tattcataga
    15841 ctctaaataa ttcgagttgc aggaaggcgg caaacgagtg aagccccagg agcttacata
    15901 agtaagtgac tggggtgaac gaatgcagcc gcagcacatg caacttgaag tatgacgagt
    15961 atggcaggag tggcagcatg caatacaacc ccttaggaaa aaccgacctt cgcgtttccc
    16021 gactttgcct cggctgtatg acctttggcg agccagatcg tggtaatcac gcatggacac
    16081 tgccggaaga gagtagccgt cccatcatta aacgcgcgct ggaaggcggc ataaatttct
    16141 ttgataccgc caatagctat tccgatggca gcagcgaaga gatcgttggt cgcgcactgc
    16201 gggatttcgc ccgtcgtgaa gacgtggtcg ttgcgacgaa agtgttccat cgcgttggtg
    16261 atttaccgga aggattatcc cgtgcacaaa ttttgcgctc tatcgacgac agcctgcgcc
    16321 gtctcggcat ggattatgtc gatatcctgc aaattcatcg ctgggattac aacacgccga
    16381 tcgaagagac gctggaagcc ctgaacgacg tggtaaaagc cgggaaagcg cgttatatcg
    16441 gcgcgtcatc catgcacgct tcgcagtttg ctcaggccct gaagctacaa aaacagcacg
    16501 gctgggcgca gtttatcagt atgcaggatc actacaatct gatttatcgc gaagaagagc
    16561 gcgagatgct gcctctgtgt tatcaggagg gcgtggcggt gattccgtgg agcccgctgg
    16621 cgcgggggcg actgacgcgt ccgtggggag aaactaccgc acgactggtg tctgatgagg
    16681 tggggagaaa tctctataaa gaaagcgatg aaaatgacgc gcagatcgca gagcggttaa
    16741 cgggcgtcag tgaagaactc ggtgcaacac gagcacaagt tgcgctggcc tggttgttga
    16801 gtaaaccggg cattgccgca ccgattatcg gtacatcgcg ggaagaacag cttgatgagc
    16861 tgctgaacgc ggtggatatc actttgaagc cagaacagat tgccgaactg gaaacgccgt
    16921 ataaaccgca tgcggtcgta ggatttaaat aagagtccag gcctgatgag acgtgacaag
    16981 cgtcacatca ggcatcggtg cacaactacg acagaatacc cagcggccag tgatgaccga
    17041 taaagtacag gatgcctgcg gaaatcaccc cggcgacaat atcgtcgatc atgatcccca
    17101 tgccgccatg tacattgcga tcaaaccagc ggatcggcca cggcttccac atatccagaa
    17161 tacggaaaat cacaaacccg gcggcaaccc actgccagtc attggtcggc agcgccatga
    17221 gcgtgatcca cataccaata aattcgtccc agacaatgct gccgtgatcg tgcacgccca
    17281 tatctttcgc cgtttgatga caaagataga cgccgataca gatccccaac atcaccacca
    17341 gcgagtagag ctgccagggt aaaaaggtca tcagatacca gaacggaatc gctgccagcg
    17401 atcccatcgt gccaggaacg attgggctta atccacttcc gaatccgaca gcaagcagat
    17461 gccacggatt actcatcttc aggcgacttt tcgcgacatc tttatggcgt ggcaaaatgg
    17521 tcatatcctt tccagtcaaa cgtgacaggt tcgccgtcac gaataaaaca aagcccttcg
    17581 atatcggcgg tcatttgccc gatacaggta aacggtacgc ccaggtgtcc gagagccaca
    17641 tccagcgcgc cacggttcag ttccggcaca gtgaaacaca actcgtaatc ttcaccgcca
    17701 gagagcgccc agcgcagcgc ctgttccggt tcaacatggc gagaaagcgc atcagaaaac
    17761 ggcagcaatg ccaggtcaat acgtgcgccg cagtcgctgg ctttcacgat atgcccgaga
    17821 tcggaaatca gaccgtcaga gagatcgata gctgaatttg ccagatcgcg cagtgcctgt
    17881 ccctgtaaaa tacgcggcga tggacggaga tgacgtttga tcaagtagtc cgcatcttta
    17941 gcatcggcaa cctgcaaacg gttttgcaaa atcgccagcc cggcggcgct atcgcccggt
    18001 gtaccggtca catagatcca gtcacccggt ttcgctccag agcgcgttaa ggctcgtccc
    18061 atcggaacaa agccgtggat acccaacgtc attgataatg gcccacgcgt ggtatcgccg
    18121 ccaatgagtt gcatatcgta ataattgaga agatcaaaca aactgtcgct gaaggactca
    18181 agccacgctt cgtctacgtc cggtaaggtt aatgccagcg tcagccaggc cggatcggcc
    18241 cccatcgctg ccagatcgct taggttcacc gccagtgctt tatacgccag atcagcagga
    18301 tcgatatcat ggaggaagtg gttgcccgcc accagcgtat cagtgctgat cgccagggtc
    18361 tgtttctcgg ggatattgag aagagcgcaa tcgtcgccga tgcccagttc gacatcaaga
    18421 cgagaacttc ttacacggtc aaaataacgg gcaatcaggg agaactcgcc acatgccata
    18481 cgttatgcct cagcaggtaa aaaagaaaag gccggcgatc gcggaacggt cttccgtgaa
    18541 tctaccggcc tggatatcac tttttgttag ggcgaatcac aggtgctgct ttatcgagta
    18601 cgccgttgac gaacttatgg ctgtcttctg cgccgaacga tttcgccagt tcgatcgctt
    18661 cgttaatggc cactttgtat ggcacatcgc tacgtttaga cagctcgtac agcgcaatgc
    18721 gcagtactgc tttttctacc tgtcccagtt cttccagcag gcgggacagg tatggcttca
    18781 tcagtccgtc gaggtatgcg gtgttagtcg ccaccccggc cagcagctca cggaagtaca
    18841 ggacgtcaac gtcttttaca tcctgttcag ccaggaactg gtattcaaca tcagcgatgt
    18901 cgttctggga caactgccag gagtagagcg cctggacggc acactcacga gcgcggcgac
    18961 gagcagcagg tttcacggat ttccccttac taatttcagg ccttgatggc tttcaataca
    19021 ttaatcattt caagcgcggt cagtgcagct tctgcacctt tgttgccagc tttggtgcca
    19081 gcacgttcga tcgcttgttc aatgctttca gtggtcagaa ccccaaaagc aaccggaatt
    19141 tcgctgtcct gggcaacatg cgccaggccg ttgcttgcac caccagcgac atattcaaag
    19201 tgggcagtgc caccacgaat aaccgtaccc agcgcaatca ccgcgtcgta tttaccggtt
    19261 ttagccagtg cacccgccgc cagcggcagc tcataggcac caggcaccca aacaacggta
    19321 atgttttcat cttttacctg accgatacgt ttcagtgcgt caattgcacc ttccagcagg
    19381 ctgtcattga taaagttgtt gaaacgcgcg atggtgatgg cgacgcgagc gtccggggta
    19441 gcaacgttag cttcaataat gttcattttc ttccttcggg ttcgagtatg gccccgcagg
    19501 ggggcggatt ttagcataat atttcgtgcg ctgcttccct ttcgagccgg gagatcatgc
    19561 acccactaaa tgcaggcaaa catccgggcc tacatgacgt atctctttga atttaaattg
    19621 gggggcgtcg gctaatttct caagccctgg cagcgtgcat aatccgcggg cgtcgctgcc
    19681 taatagttta ggtgcgatat agacaatcag ctcatcgact aaacccgcct gcagcaatgc
    19741 gccagcgagc gttggccccg cttccaccca gatgctgtta atttgctgtt tacccagttg
    19801 catcatcagt acaaccagat ccagatgacc tttatgctct ggaatcagca aggtacgcac
    19861 cgtttccggc cactcactag aatcttcctg cgtacgcgcg aaccaggttt cgccgggctg
    19921 ctgcacaatg cgatgttccg gcgtcacgcg attttggcta tcaatcacaa tacgtactgg
    19981 ctggcggaga ttttgttgcg ggtagagcgc ctgagtttgt tcatccagtt cagaccaacg
    20041 cactgttaag gctggatcat ccgccagcac cgtggcgctg ctggttaaaa tggcatgact
    20101 ttgtgcgcgc tgtcgttgta cgtcgcgccg cgcctgaggc gaagtgatcc actggctttc
    20161 gccgctcgcc atcgccgtgc ggccatcaag cgatgcgcca agttttaact gaatataagg
    20221 aaagccggtg cgcatccgct tgagaaagcc tttattcaat tgctcggctt cactcatcat
    20281 cagcccgtgg ctgacgtcaa tgccagcctg ttgcagacgg taaagtccac gcccagcgac
    20341 ctgcgggtta ggatcttgca tcgaggcaac cacgcgcgct acgccagcgg cgatgagtgc
    20401 gtcacagcac ggtggcgtac gaccatgatg gctacagggt tcgagtgtca catacgccgt
    20461 tgcgcctttg gctttttcac ccgccatacg caacgcgtgt acttcggcat gtggttcacc
    20521 cgctcgttgg tggtaacctt caccgacaat ttcgccatct ttgacaatga cgcacccgac
    20581 attcgggttg gggtgggtgg taaaacgtcc tcgttgcgcc agctttagcg cccgcgccat
    20641 gtaatactcg tcctgcacgg cttagtcctc caggcgcgcg atctcttcgc caaattcttt
    20701 gatatcttcg aaactgcggt agacagaggc aaaacggata taggcgactt tatcgagctt
    20761 tttcaattgc tccatcacca gattgccaat catcttgctc ggcacttcgc gctcaccggt
    20821 ggcgcgcagc tgcgatttaa tatgattgat tgccatttcg acgtcatcgg aactcaccgg
    20881 acgtttttcc agcgcccgca gcattccgct acgcaatttc tcttcattaa acggttcacg
    20941 cacgtcgttg ctttttacaa cacgcggcat aaccagctcc gccacttcaa aggtggtgaa
    21001 acgttcatta cacaccagac actgccgacg gcggcgtacg gatgaaccct cgcccacgag
    21061 acgagagtca attaccttag tgtccacggc gaaacagaat gggcaatgca tacggcgtcc
    21121 tgaccaggtg gttaacagaa atctatttta ccctgaactg acgatacaac aaaggcgcaa
    21181 cgcttttgag ataaaggatc gatgctttcg ctgcttttac cctctaccat taggctaatt
    21241 gcgacgagaa agggacgacc tatgaatact aacgtttttc gactgctcct gctgggaagc
    21301 ctgttcagcc ttagcgcctg tgtgcagcag agcgaagtgc gacagatgaa acacagcgtc
    21361 agcacgctga accaggagat gacgcaactc aataaagaaa cggtcaaaat cactcagcaa
    21421 aacaggctga atgcaaaatc cagcagtggg gtttaccttc tgccaggagc gaaaacaccg
    21481 gcaagactgg aaagccagat cggtacttta cgtatgtcgc tggtgaatat tacgcctgat
    21541 gcagatggca ccaccctgac gctacgtatt cagggcgagt cgaatgatcc cttgcccgca
    21601 ttcagcggaa ctgttgaata tgggcaaatt cagggaacaa tagacaactt tcaggaaatc
    21661 aatgtgcaga atcaattgat taatgcccct gccagcgtcc tcgcccccag cgatgttgat
    21721 attccgttac agttaaaggg tatttctgta gaacagttag acttcgtacg tatccacgat
    21781 attcaacctg ttatgcagta aacgtatttc ggggcgattt tgtgcgtccc gcaacatctt
    21841 tccccgtcat tttgttactc cgcttacatc acccagattg atagtaaaag tttgcaacaa
    21901 gggtgaaagt cagtacaatc cctgcccgaa tgtgtgtaaa cgtgaacgca atcgattacg
    21961 taaatgatag aactgtgaaa cgaaacatat ttttgtgagc aatgattttt ataataggct
    22021 cctctgtata cgaaatattt agaaacgcaa tttgcgcctt tttcactccc gcaagggatt
    22081 ttcaaacagt ggcatacata tgaaaaaaac attactggca gccggtgcgg tactggcgct
    22141 ctcttcgtct tttactgtca acgcagctga aaacgacaaa ccgcagtatc tttccgactg
    22201 gtggcaccag agcgttaacg ttgtcggaag ctatcacacc cgttttggac cgcagatccg
    22261 caacgatacc taccttgagt acgaagcatt cgctaaaaaa gactggttcg acttctatgg
    22321 ttatgcggat gcgccggtat tcttcggcgg taactccgat gccaaaggta tctggaacca
    22381 cggctctccg ctgtttatgg aaatcgaacc acgtttctcc atcgacaagc tgaccaatac
    22441 tgaccttagc ttcggtccgt tcaaagagtg gtacttcgcg aacaactaca tttacgacat
    22501 gggtcgtaat aaagatggtc gccagagcac ctggtatatg ggtctgggta ccgacatcga
    22561 tactggtctg ccgatgagcc tgtccatgaa cgtctatgcg aaataccagt ggcagaacta
    22621 tggcgcagcg aacgaaaacg agtgggacgg ttaccgtttc aaaattaaat actttgtgcc
    22681 gattaccgat ctgtggggcg gtcagctgag ctacatcggc ttcaccaact tcgactgggg
    22741 ttccgattta ggggatgaca gcggtaacgc aatcaacggt attaagaccc gtactaataa
    22801 ctctatcgct tccagccata ttctggctct gaactacgat cactggcact actctgtcgt
    22861 agctcgttac tggcacgacg gtggtcagtg gaacgacgat gcagaactga acttcggcaa
    22921 cggcaacttc aacgttcgct ctaccggctg gggtggttac ctggttgtag gttacaactt
    22981 ctgattatga aaatgccggg atttattccc ggcatttcag attgttaaca acgtgcgctt
    23041 tgttcatgcc ggatgcggcg taaacgcttt gtccgaccca caaaagcacg taaattcaat
    23101 atattgctga aataacgtag tcctgataag cgtagcgcat caggcaattt tacgttttaa
    23161 tcacttcttc ttgttcatca tcgctttcag gtcagcgaat gggttgtact tcgcttcacc
    23221 gacatctttc tgcgcatctt cccctgcgat aacggtcgta ccatactgat ccgcttcggt
    23281 atatttcgaa tgctcatgat cgtggcaata gagacaaagc aattcccagt tactgccatc
    23341 ttccgggtta ttggtatggt cgtgatcaat gtggtgaaca gtaagttcac gcaggttgga
    23401 atagacaaac tcgcgggaac agcgaccgca gacccacgga tagattttta atgctttttc
    23461 gcgatagccg ctttctaacc gcgcatagtt ttttgggatg atagccatga aaagttacct
    23521 ggatgaaatg tagggttacg cgcagtttcc caagatgcgg ggattttcgc aatctccata
    23581 catcaaaaaa aatcccgatc ttctgaccgg gattttcaac atcaacggga acttgattaa
    23641 ggcagaattg acggctgatc cgccccttct ttttccactt tctgctgcaa catgtgttcg
    23701 cgcttcatac ccagtttcag agccaacgca gatgccacat agatggaaga tgcagtaccg
    23761 atggaaacac cgataagcat ggtcagcgag aagccttcca gtaccggacc accgaagagg
    23821 tacagcatca ggataaccat caaggtagta ccggatgtga tcaaggtacg gtgcagcgtc
    23881 tgggtcaagg acacgttaaa gatttcgtaa ggcgtaccgc gacggatctt gcggaagttt
    23941 tcacgaatac ggtccgatac cacgatactg tcgttaagcg agtaaccgat aaccgacatc
    24001 aacgatgcca caatggtcag gtcaatctcg atatggaata acgacaaaat acccagcgta
    24061 atgataacgt cgtgcgccag cgcaataacc acccctgccg ccagtcgcca ctcaaagcgg
    24121 aaacctacgt acacgaggat agacagcagc gctgccatca acgccatcgc accggtttgc
    24181 gcaaggtctg cccccacgct cggaccgacg aactcaatac gcttcactgc tgcattctga
    24241 ttggtggatt cgttaatcac cttcagaacc tggctgccca gcacctgacc gccggtttcg
    24301 ccttcagcag gcggcatacg gaccatgatg tcgtggctgc taccaaagtt ttgcagcatt
    24361 ggctcttcaa aaccggcttt ttgcaatgca tcacgcatta cgtcaatttc agccggtttt
    24421 tcgagcgtaa tttcgataac cgtaccaccg gtaaaatcca gcccccagtt aaagccgcgc
    24481 acgcccataa taacgatagc agcgattaac agcagaccag agatgccgaa agcccagtag
    24541 tcccagcgca taaagtcata gactttacgg ccgtggttta gttgttcaac agtatattcc
    24601 tgtgccacat cgcactcctc agattgacag cttcttgacg cgcttgccgc catatagcag
    24661 gtttacgatg gcacgcgtac cgacaatcgc ggtaaacatc gacgtcgcca caccgatacc
    24721 ggtagtaatc gcgaaccctt taattgcccc ggtacccact gcgtacagga tgatgacttt
    24781 aatcagcgtg gtgatgttcg catcgaagat agaactgaat gcgccacgat aaccttcatc
    24841 aattgcctgt tgaacagtac gtccgttgct caactcttct ttaatacgtt cgttgatcag
    24901 tacgttcgca tcgaccgcca ccgcaagggt taagacgata cccgcgatac ctggcatact
    24961 cagcgttgcg cctggcaaca gcgacataat gccgacgatt aagatcaagt tggcaatcag
    25021 agcactggtc gcaatcagac caaacttctt atagaagatg atcatgaaca gaatagaaac
    25081 cagcagaccg gcaaggcaag cctccagccc ctgttcaatg ttctgcatac ccagggttgg
    25141 gccaatggta cgttcttcaa caatctgaat cggcgcgatc aacgcaccgg cacgcagcag
    25201 cagtgacagc tgacgggctt cgttcgggtt gttgatgccg gtgatacgga agctgttacc
    25261 cagacgagac tggatgttgg cgatgttaat cacctcttcc tgtttcacca gaaccgcacg
    25321 accatttgca tctttcttac cgctgtcttt gtactccaca aacagggttg ccatcggttt
    25381 gccgatgttg tccttagtga agttagacat gatgttacca ccagcgctat cgagcgagat
    25441 gttaacctgc ggttggttgt attcgtcctg gctggaagtg gagtcggtga tatggtcacc
    25501 ggtcagaatt acgcgtttgt acagcacaac tggctgacct tcgcgggtct gtttcacttc
    25561 agagtcgccc ggtacgcgac cggatgccgc agcggcctgg tcaacgttgg tgtttaccag
    25621 acggaattcc agcgttgccg tcgcacccag aatctctttc gcacgcgcag tgtcctgaat
    25681 acctggcagt tcaacaacga tacggtcagc accctgacgc tgaaccaccg gctccgccac
    25741 gccaagttgg tttacacggt tacgcaggat attaatgttc tgctgcaccg catattcacg
    25801 cgcttcactc agacgagcat cgctcattac cgcacgcagc tggttgctgc cctggctgct
    25861 aatcaccagg tccggatggc gcttgctcag atacgcaatg gcttcatcac gagctttagc
    25921 atcgcggaaa gtaatgctca ggccgtagtt gttttcttta cgaacggtgg tatacgggat
    25981 gcccttttca cgcagatcgc tgcgcaggct atcgatattt tgttcctgga gtttgccaag
    26041 cgcggtgtcc atatccactt ccatcaggaa gtgaacgccg ccacgcaggt caaggccgag
    26101 cttcatcggc tcagcgtgaa tagctgccag ccagcgcggc gttgccgggg caaggttaag
    26161 cgccacgacg tatttgtcac ccataacgcc cattaatgct tcacgagcgc gcagctgggt
    26221 gtcagtggag tcgaagcgcg caagaatagc gccctcttcc agtgccacag acttagcagt
    26281 tattttttct tcttgtaacg ttttctggac ctggatcagc gtttgctcac tggcggcgac
    26341 tccgcgcgca ccagtgatct gaacagccgg atcctcacca aacaggttgg gaagcgcata
    26401 cagcagaccg atgacaatca ccacgatcag catgacgtac ttccacaaag gataacggtt
    26461 taacacggca attcccttag ggaaaaattt taattacagc gccttcatgg tgcctttcgg
    26521 caggacggca gctacgaagt cacgtttaat aactacttca gtggtgtcat tcagcgcgat
    26581 agcaatgtag ccgttttccg ctactttggt tacgcggcca accaggccac cgttcgtcag
    26641 aacttcatca cctttggcaa tggagtccat cagctttttg tgttctttgg tgcgcttctg
    26701 ctgtggacgc aggatcatga aatagaaaat cagaccgaat accaccagca tcaaaatcaa
    26761 agacatcggg ctaccttgcg ccggtgcacc cgttgccgct accgcatcag aaataaaaaa
    26821 gctcattaaa tttccctcat tattaatatt aatcaacgtt caaaggtggt acttctcgcc
    26881 cctgacgctg gtaaaaatca gttacgaagc tctctaattt accctcttca atagccttgc
    26941 gtaaacccgc catcaaacgc tggtagtaac gaaggttatg aatggtgttg agtcgcgcgc
    27001 ctaatatttc gttgcaacgg tcaagatgat gcaagtaagc gcgtgaataa ttgcgacagg
    27061 tgtagcaatc acactcagga tcgagtgggc cagtatcgct cttatacttc gcattgcgga
    27121 ttttcaccac gccatcggtc acgaacaaat gaccatttcg ggcgttgcgg gttggcatta
    27181 cgcagtcaaa catatcgata ccacgacgta cgccttcaac caggtcttct ggtttaccaa
    27241 cgcccatcag gtaacgcggt ttgtctgccg gaatttgcgg gcatacatgc tccagaatgc
    27301 ggtgcatatc tgctttcggc tcacccacag ccagaccgcc gacagcgtag ccatcaaaac
    27361 cgatatctac cagaccttta acagaaatat cacgtaaatc ttcgtaaacg ctgccctgaa
    27421 tgataccaaa cagcgcattt ttgtttccga gactgtcaaa acgctcacgg ctacgcttcg
    27481 cccaacgcag agacatctcc atggagcgtt ttgcgtaatc ccagtcagca ggatacggcg
    27541 tacactcatc aaagatcatg acgatatccg aaccaagatc gtactgaatc tccattgatt
    27601 tttcaggatc gaggaaaatc ggatcgccgt tgatcgggtt acggaagtgc acgccctgtt
    27661 cggtgatttt acgaatatcg ccaaggctga agacctggaa accgccggag tcggtgagga
    27721 tcggcccctt ccactgcata aaatcgtgca gatcgccgtg cagtttcatg atttcctggc
    27781 ccgggcgcag ccacaggtgg aaggtgttgc cgaggataat ttgcgcgcca gtggcttcaa
    27841 cttcttccgg cgtcatccct tttacggtgc cgtaggtgcc aacaggcata aaacaaggcg
    27901 tttccactac gccacgatca aagaccaggc ggccacggcg tgcgcgaccg tcggtggtgt
    27961 ccagttcaaa tttcattttt tctccactac gtcagaaaaa cagtccaacg ttttaaacca
    28021 gcgccgcgga attactcccc gacgcgctca ttaattgcct gcggattgta cgtgataaac
    28081 atcgcatcac cgtaactaaa aaagcgatat ttctcttcta ccgctgcttt ataggcgttc
    28141 atggtgtgtt gataaccggc aaaggccgaa accagcataa tcagcgtcga ctctggcaag
    28201 tggaagttcg tcaccagcgc atcgaccact ttgtactgga agcccggata gataaagatt
    28261 tgggtatcgt cgaagaacgg ttcaatgaga tcgtttttcg ctgcctgagc cgcgctttcc
    28321 agcgaacgta ctgaagtggt gccaaccgca atcacccggt taccgcgcgc tttcgccgcc
    28381 agtaccgcgt ctaccacatc ctgcggtact tcagcgtatt ccgagtgcat gatgtgatct
    28441 tcaatggtgt cgacgcgcac cggctggaag gtgcccgcac caacgtgcaa cgtcacaaac
    28501 gccatctcca cgcctttggc gcgcaatttt tccagcaaag gctcgtcaaa atgcagacca
    28561 gcggtcgggg ctgcaaccgc gcccggtttt tcgctataaa cggtttgata aagttcgcgg
    28621 tcagcgtctt cgtccggacg gtcgatatac ggcggcagcg gcatatggcc gatactgttg
    28681 aggatatcca gtaccgaacg atcatcattg aattcgactt caaacagtgc gccgtggcgc
    28741 gcggtcattg ttgcgttaat actttcgtca tcgcccagca gcagttctgc gccaggtttt
    28801 ggcgctttcg aggcgcgaat atgcgcaaga atgcgtttgt cgtcgagcat ccgctcaacc
    28861 agcacttcaa tcttgccgcc gctggcttta cgcccaaaca ggcgcgccgg gatcacgcgg
    28921 gtattattaa aaaccagaag atcgccgggg ttgagcttat caagtaaatc ggtgaaagta
    28981 ccgtgcgtca gcgcgcccgt cggcccgtcc agcgacagta aacgacagct actgcgttca
    29041 ggcatgggat agtgggcaat cagggattcg ggcaattcaa aggagaaatc ggtaacgcgc
    29101 atgacactga ctcagactaa aataagaggc gggtagtcta gtgccggggc gctctccctg
    29161 caacctttac ccccttttgg atagaatata atcgatgaat tttttagctc acctgcattt
    29221 agcccatctc gcggaaagct cgctttccgg caatttactg gctgatttcg tacgcggaaa
    29281 tcctgaagaa agttttccgc ccgacgtcgt ggctggcatt catatgcatc gacgtatcga
    29341 cgtattaacc gacaatctgc cggaagtccg cgaagcacgg gaatggtttc gtagtgaaac
    29401 tcgccgcgtt gcgcctatta cgctggatgt catgtgggat cactttcttt cccgccactg
    29461 gtcgcagctg tcgccggact ttccgctaca ggaatttgtc tgttatgccc gcgagcaagt
    29521 gatgacgatt ttgccggact caccgccacg ttttatcaat ctgaacaatt acttgtggtc
    29581 agagcagtgg ctggtgcgct atcgcgatat ggatttcatc cagaacgtgt taaacggcat
    29641 ggcaagccgc cgcccacgtc tggatgccct gcgtgactcc tggtacgatt tagacgctca
    29701 ttatgacgcc ctcgaaaccc gcttctggca gttttatccg cggatgatgg cgcaggcgtc
    29761 acacaaggcg ttataaaacg ttagttcatc cataccgtag ccgaaatggc aggcaaagcc
    29821 agaatcccgt ttgtcagttg cccatggcct tctttgcgtt gccattgcgc gacattaaga
    29881 aacggtgacg cggggagcac cacttcacag gcctcgccac ggttgattgc caccagtaca
    29941 cgttgctgat tcagcacgcg gacaaatacc actacgttat cttccgcata cagcacctga
    30001 cagccgccac gacgcagcgc ctgacttttc ttacgcagcg caatcattcg ctggtacagc
    30061 gcgaataacg ccgtatcctg cttttccacc tgccagggga acggtttacg gcaaaacgga
    30121 tcgtttttgc catccagtcc tacttcatca ccgtaataaa tgcacggtac accaggccag
    30181 gtgaacagcc agaccaccgc cagcggcagg cgcgcaatat cccgaccgag cagcgtttta
    30241 aatcgcgcag tatcgtggct gtcgagctga ttaaacatac gtaattgttg ttgatgagaa
    30301 agccctgcgc ggtaattatc catccaggcc atacaggttt gggcatcaat ttgctgcgga
    30361 tcgtaagaga tatcggtatt ggcaagaaat ccccacaacg ggaatgtgaa gccacgatag
    30421 ttcatggcgg catcttccac atcggcctgt aaccattgcc gtgcatcgcc aaaatgttcg
    30481 ccgacaatat acgcttccgg ctgggtttct ttcgccgctt cggtgatccc agcaacgtgc
    30541 tgcatattat tgcgcgcccc acccgcctcc cccagcatat gcaccacatc cagccgccag
    30601 ccgtccatac tccacggcgc tttcagccaa tggcggacaa tactgtcttc cccgcgataa
    30661 atttcattca ccagactttc cgagtgataa tccagcttcg gcaagctggc atagccaagc
    30721 cagtcgagcg ccgtgccatc atcactaaac gagtaccagt cgcgccaggg cgattcaggg
    30781 ttgtgacaag ctccccccgt gccacgattg tgcctgtcaa accaggcatg ggaatcgcca
    30841 ctgtggttaa acacgccatc cagcaccagc cgcattccca gctgctgcgt attgtggcgt
    30901 aagcgcagca acgccccatc accgccaaac tgcggatcga catggcgata atcctcggta
    30961 tcgtatttat gtacgctggg agctttaaac accggattga gatacagcgc cgtcacgcca
    31021 agctttttca gatacggcag tttttcgctt atcccgtcca gatcgccgcc atagaacgtt
    31081 gatccgcccg cctgcgccgt gaccggttca tcccagtcac gcaagatgat ctcttgtccg
    31141 gctgcatgat ggtaatagac atgatcctgt tcagcttcac gaggaagact acgcgcaaaa
    31201 cgatcaggga agatctgata aaaaatctga tccgcagccc attgtgggcc gatatccggt
    31261 acatcgacgg caaactgctc cagtcgtgcc ggcggcattc ggctgaagcc ctgcggtgta
    31321 aaccaacgct ggcgatcgtg ccacagcagt ttgaaactgt aacgccgccg gggttgtccg
    31381 ctggagagat caatcgccgc acgccatgcg gtgacgccag gctgctgctg actgcgctgc
    31441 ttatgcatcg atactgacat ttcttcgtta tcgtgttctg tacgcagcat aatgcgctgc
    31501 ggtgggtctt cgcccgtcag ccacagtgta atgagcagtt gatctttgct ttgtttaaca
    31561 aatgggggca ccggcaggtg ccatgcattt aacatcataa ttcccctgtg atgaaaatct
    31621 gatcaccttg ccatagccta atgcaggatc actcatcatc tgaaggatat ttgggggagg
    31681 aggacggggg cggataactt tcggaatagc cggatcagga tttcctcctg aaccggctta
    31741 cagaacgcaa ggattactgg ttttcagcca gctgacgatc gtggcggcgt ttaaacatcc
    31801 agccaatcaa cagcacaaca atccacgcga aaccgacata cagcgagata cgcgtatccg
    31861 ggtgataacc aatcaacccg ataataaaga gcaggaaaat caaaccgccg atggtcgttg
    31921 ctaccccacc cggcacttta aatttcagcg ccttaacttc ttctggcggc aaacggcgac
    31981 ggaaggcaat ttgcgacagc aggatcataa tccacaccca caccgtggcg aaggttgcca
    32041 gcgaagcgat caccaggaag acgttttccg gcatgatgta gttcagatac accgcaaaca
    32101 gcagcgcggt agtcatcacc agcaccgtaa cccacggaat accgcgacgc gacgttttgc
    32161 tgaaaatttt cggcgcgctg ccctgctctg ccataccgtg gagcatacgg cctacgccaa
    32221 atacgtcact gttaattgcc gacagcgaag cagtcagcac aacaaagtta agaatgctgg
    32281 cggcaaaggt aatgcccata tgctggaacg tcagcacgaa cgggctaccg gcagtgccaa
    32341 cctgattcca cgggtagata gacataatga cgaacagcgt accgacgtag aacaccagaa
    32401 tacgcatcgg cacggagtta atcgcacgcg gtatcgattt ctcaggatct ttcgcttcac
    32461 cggcggtaat cccgataatt tcgatcccac cgtaagcaaa catcaccatt tgcaacgaca
    32521 tcaccatccc aagccagccg ttactgaaga agccgccgtt gctccacagg ttatgaatac
    32581 cggtcggttg cccgccgttg ccaatccccc agatgatgat gccgaaaccg gcgacaatca
    32641 tgatgatgat ggtggcgact ttaaagaacg agaaccagaa ttccagctca ccgaagacct
    32701 tcacgctcat caggtttacg gcgcaaatga tcagcaccac gctcagtacc caaatccagt
    32761 gcggcaccgt cgggaaccag acacccatat agataccaaa agcggtcaca tcggcgatgg
    32821 cgacaataag gatttcaaag caataggtcc agccggtaat gtaacctgcc agcgggccga
    32881 ggttttcctg cgcataacgc gagaaagagc tggctgccgg gttatgtacc gacatttccc
    32941 ccagcgcacg cataatgata tacgccgcga taccaccgat aatataggcc aacaacacgc
    33001 tcggaccggc cattttgatg gcgtctgccg aaccgtaaaa cagcccggtg ccaattgctg
    33061 aacccagtgc cataaagcgt atgtggcggg tacttagccc acgctttagc ttgttcttac
    33121 tttccatcaa ttcctaaccc atcaacacaa taaaaaacca cggggcatcg cagccccgtg
    33181 gttaaaacaa atgttcagtg atttagtgag cgctggaggt cacctgacga cctgccgcgc
    33241 gatcccagat aatggccaga accaccatca ccactgttgg cattaaccac gccagacctt
    33301 gttcggccag cggtaaacgc tgcgcccagg acggtaagat atcgctgaat gcagatgctt
    33361 tgatcccgtc gagaatacca aaaagcaggc tgataaacat cggcggagca atcacgcggg
    33421 acgaattatg ccaccatgag cgtgtaaaac ttaatacaac cagtgcgata cacggcggat
    33481 aaatagcggt cagcaccggt acggagatct ggatcagctg gcttaagccg aggttagaaa
    33541 ccaccatcga gaagccgccg aggataaaca ccagcgtacg ataagagagc ggtacgtact
    33601 gggcaaagaa ttctgcacaa gcacaggtca ggccaactgc cgttaccagg caggcgatga
    33661 agattaacgc cgccagcagg aagctaccgc cgccgccaaa ggtgtgctga acgtaagcat
    33721 gcagaatagc agcgccgttt gcagactgat cgaccagcga cgcgctgtct gaacccagac
    33781 ggaacagcgc caggtacagc agagtcagac caacacccgc catcaggcca gcccagacgg
    33841 tataacgggt cagcagacgc gcttcggtaa cgccacgaga acgcgccgcg ttaacaataa
    33901 cgataccaaa caccattgcg cccagcgtat ccatggtcag atagccgtta acgaagccgt
    33961 tagaaaacgc agcgttttga taagcctcag tcgccgtgct gatagaaccc gccggccaga
    34021 caatcgcggc aacagacagg atgaccagcg cgataatttt cagcggcgca aggaagttgc
    34081 ccacggtatc cagcagcttg cccggataga gcgaaaccag aataacgata gcgaaataga
    34141 ccaggctgta gataaacagc ggcagcgcgg aatcaccagt cagcggcgca atccccactt
    34201 caaaggaaac ggtagctgta cgcggcgtag cgaaaagcgg ccccaccgcc aggtaacaaa
    34261 ccgttgccag cagtacgcca gcgactttac cgattggcgt gctgaggctg tcaacaccgc
    34321 cgccaacttt tgccagcgcc actaccgtta gtaccggcag accaacggca gtaatgagga
    34381 agccgaatgc cgcagtccag acgtgttcgc ctgcctgcaa gccgaccatt ggagggaaaa
    34441 taatgttacc tgcgccgacg aacaacgcaa atgtcataaa gcccagagcg atgatatcgc
    34501 gcgatcttaa ttgatgggtc ataaatcata ctgcctgtgg atgtggtgtt gtaacgttaa
    34561 atttttgtgc caatccctgg ggatgataca gcggtatatt taaacaattt tagcgggaaa
    34621 tactcccaac caggcgcggc gaagaatagt ttttcgattt accgcgttaa tgcagtcagt
    34681 atgacagcac ctgaagcgca atttaaacgc ttataacgtt ttaaagcaag gagggatcta
    34741 aaaaccagag gtatatagcg ataagaaatc accaaccagt ataaaacact atttccgtga
    34801 aaaagacatg gctaatcatg cgaacaaaaa atcaccaacc gttgtaaaat ctaacgcccg
    34861 tattttgact gcaaagaaaa taagccagca tcggctggct tatggcaata aaagatgaca
    34921 aaggcggatt aatcgctgtt tttggcaatt aaacgttccg ggataacaaa actgaaacgt
    34981 gttccttttc ctactgtact ctcaatattc aggcgacttt cgtgatgatt caccgcatgt
    35041 ttcacgatcg ctaaccctaa tccgctaccg ccggtttgcc gggaacgcgc tttatcaacg
    35101 cgataaaaac gctcggtcag gcgcggaata tgctccggtg caatgcccgg tccgttatct
    35161 tcaacgctaa attcggcacc gtgcggcact cgctgccagc gtacggtgat atgcgtgcct
    35221 tctggagtat gattcacggc gttatagacc aggttcgaaa tcgcactgcg tagctgatct
    35281 tcgttgccag acactttgag gccgttatct atctcaaagg taaatgtctg ttttttctga
    35341 ctcagagtct gagcctcacg ctcaacaacg cgcagcatca tcggcacatc aaccttttca
    35401 ttgagcaaat gcgtcggtgc ggcttctatt ttcgacagcg tcagcaactg cttcaccagc
    35461 ccttccatcc gctgggtctg ctcgcgcatg gtgtgcaacg ctttttcgcg taccgcgcct
    35521 tccagcggct gctcatccat catctccagg taaccctgta acacggtcaa tggcgtgcgt
    35581 aactcatggc tcacgttggc aaaaaagtta cgccgcgccc cttccagttg atgcatttgc
    35641 gtgacatcac gcgccaccat cagcaactgt ttgtgggtat aaggcatgac gcgaatttcc
    35701 agatgccgcc cggtgttgag caccagattg agcaggcgag aaaaatcacg cgttttcaga
    35761 tattgcgtaa actccgggta acgcagtagg ttaaggatgt tctgcccgtt atcttccggc
    35821 cagcgcaaac caagaatttg ttgcgccaga ccgttacacc agaaaatacc gccctcttcc
    35881 gtggtcagca ccaccgcgtc gggcagcgac tccgcgccgc tacgaaagcg tttaatcaga
    35941 ttgcccagtt cacggcggcg ttttttattt cgcagctgca tctggtgtaa gccgtatagt
    36001 agcggttccc agctaccacg ccccggtggc ggggtcatac tgcgatccac ccacagccac
    36061 catgaaaggc gcaataaatt ccagaaatgc cagataagca gtcctgttac cgatgccagc
    36121 aaaaaccagg gcaggtaacc aaaaaatgca cccaggatga aagccgggag gcagcaaagt
    36181 agcagctcca gcaccagcct tttccacgac agccgttcca gcacgtaaga tactccagtt
    36241 aagaaatcat aagccctgct ctgcgtccga tgagcaaggc gttaaaagcg ggttgaaaaa
    36301 cgatatcctg taccgcgcac ggtctgcacc atgcggtcat gcccgccggg ctccagtgct
    36361 ttacgcaggc gacgaatgtg gacatcgacc gtgcggtctt ccacatacac gttagttccc
    36421 cagacgtggt ttaacagctg ctcgcggctg tacacgcgct caggatgcgt cataaaaaag
    36481 tgcagcagtt taaattctgt cggccccatc tccagcggct cttcgcccgc catcactcgg
    36541 tgagatgtcg ggtcgagact taatccctgc atctcaatca cctcttccac cgccattggc
    36601 gaaatacggc gcattaccgc tttgattcgc gccaccagct ccttcggcga aaacggcttg
    36661 gtgatatagt catccgcgcc ggtttcaagg ccgcgcacgc gatcttcttc ttcccctctg
    36721 gcggtcaaca tcaccactgg aatatcccgg gtcatcgact cgcgcttgag gtgtttgatg
    36781 aactggatac cggagccgcc aggtaacatc cagtcaagga gaattaaatc cggccagggt
    36841 tcattcagtt gattcacagc actgtcataa tcttccgctt cgaccggctg aaagccattt
    36901 tgttcgagca cgaagcagac catttcgcga attggagctt catcttctac gaccagaata
    36961 cgtctcgcca tgatttgccc tgttgtaata aataggttgc gatcattaat gcgacgtcat
    37021 tatgcgtcag atttatgaca gatttatgaa aagctcgtcg cacatatctt caggttattg
    37081 atttccgtgg cgcagaaaaa agcaaatggc acatctgttt gggtataatc gcgcccatgc
    37141 tttttcgcca gggaatcgtt atgcgcatcc ttcacacctc agactggcat ctcggccaga
    37201 acttctacag taaaagccgc gaagctgaac atcaggcttt tcttgactgg ctgctggaaa
    37261 cagcacaaac ccatcaggtg gatgcgatta ttgttgctgg tgatgttttc gataccggct
    37321 cgccgcccag ttacgcccgc acgttataca accgttttgt tgtcaattta cagcaaactg
    37381 gctgtcatct ggtggtactg gcaggaaacc atgactcggt cgccacgctg aatgaatcgc
    37441 gcgatatcat ggcgttcctc aatacaaccg tggtcgccag cgccggacat gcgccgcaaa
    37501 tcttgcctcg tcgcgacggg acgccaggcg cagtgctgtg ccccattccg tttttacgtc
    37561 cgcgtgacat tattaccagc caggcggggc ttaacggtat tgaaaaacag cagcatttac
    37621 tggcagcgat taccgattat taccaacaac aatatgccga tgcctgcaaa ctgcgcggcg
    37681 atcagcctct gcccatcatc gccacgggac acttaacgac cgtgggtgcc agtaaaagtg
    37741 acgccgtgcg tgacatttat attggcacgc tggacgcgtt tccagcacaa aactttccac
    37801 cagccgacta catcgcgctc gggcatattc accgcgcaca gattattggc ggcatggaac
    37861 atgttcgcta ttgcggctct cccattccac tgagttttga tgaatgcgga aagagtaaat
    37921 atgtccatct ggtgacattt tcaaacggca aattagagag cgtggaaaac ctgaacgtac
    37981 cggtaacgca acccatggcg gtgctgaaag gcgatctggc gtcgattacc gcacagctgg
    38041 aacagtggcg cgatgtatca caggagccgc ctgtctggct ggatatcgaa atcactactg
    38101 atgagtatct gcatgatatt cagcgcaaaa tccaggcatt aaccgaatca ttgcctgttg
    38161 aagtattgct ggtacgccgg agtcgtgaac agcgcgagcg tgtgttagcc agccaacagc
    38221 gtgaaaccct cagcgaactc agcgtcgaag aggtgttcaa tcgccgtctg gcactggaag
    38281 aactggatga atcgcagcag caacgtctgc agcatctttt caccacgacg ttgcataccc
    38341 tcgccggaga acacgaagca tgaaaattct cagcctgcgc ctgaaaaacc tgaactcatt
    38401 aaaaggcgaa tggaagattg atttcacccg cgagccgttc gccagcaacg ggctgtttgc
    38461 tattaccggc ccaacaggtg cggggaaaac caccctgctg gacgccattt gtctggcgct
    38521 gtatcacgaa actccgcgtc tctctaacgt ttcacaatcg caaaatgatc tcatgacccg
    38581 cgataccgcc gaatgtctgg cggaggtgga gtttgaagtg aaaggtgaag cgtaccgtgc
    38641 attctggagc cagaatcggg cgcgtaacca acccgacggt aatttgcagg tgccacgcgt
    38701 agagctggcg cgctgcgccg acggcaaaat tctcgccgac aaagtgaaag ataagctgga
    38761 actgacagcg acgttaaccg ggctggatta cgggcgcttc acccgttcga tgctgctttc
    38821 gcaggggcaa tttgctgcct tcctgaatgc caaacccaaa gaacgcgcgg aattgctcga
    38881 ggagttaacc ggcactgaaa tctacgggca aatctcggcg atggtttttg agcagcacaa
    38941 atcggcccgc acagagctgg agaagctgca agcgcaggcc agcggcgtcg cgttgctcac
    39001 gccggaacaa gtgcaatcgc tgacagcgag tttgcaggta cttattgacg aagaaaaaca
    39061 gttacttacc gcgcagcagc aagaacaaca atcactaaac tggttaacgc gtctggacga
    39121 attgcagcaa gaagccagcc gccgtcagca ggccttgcaa caggcgttag ccgaagaaga
    39181 aaaagcgcaa cctcaactgg cggcgctcag tctggcacaa ccggcacgaa atcttcgtcc
    39241 gcactgggaa cgcatcgcag aacacagcgc ggcgctggcg catactcgcc agcagattga
    39301 agaagtaaat actcgcttac agagcacaat ggcgcttcgc gcgagcattc gccaccacgc
    39361 ggcgaatcag tcagcagaat tacagcagca gcaacaaagc ctgaatacct ggttacagga
    39421 acacgaccgc ttccgtcagt ggaacaacga actggcgggt tggcgtgcgc agttctccca
    39481 acaaaccagc gatcgcgagc atctgcggca atggcagcaa cagttaaccc atgctgagca
    39541 aaaacttaat acgcttgcgg cgatcacgtt gacgttaacc gccgatgaag ttgctagcgc
    39601 cctggcgcaa catgccgagc aacgcccact gcgtcagcgc ctggtcgcgc tgcatgggca
    39661 gattgttccc caacaaaaac gtctggcgca gttacaggtc gctatccaga atgtcacgca
    39721 agaacagacg caacgtaacg ccgcacttaa cgaaatgcgc cagcgttata aagaaaagac
    39781 gcagcaactt gccgatgtga aaaccatttg cgagcaggaa gcgcgcatca aaacgctgga
    39841 agctcaacgt gcacagttac aggcgggtca gccttgtcca ctttgtggtt ccaccagcca
    39901 cccggcggtc gaggcgtatc aggcgctgga gcctggcgtt aatcagtctc gattactggc
    39961 gctggaaaac gaagttaaaa agctcggcga agaaggtgcg gcgctgcgcg ggcaactgga
    40021 tgcattaacg aagcagcttc agcgcgatga aaacgaagcg caaagcctcc gacaagatga
    40081 gcaagcactt actcaacaat ggcaagccgt cacggccagc ctcaatatca ccttgcagcc
    40141 acaggacgat attcaaccgt ggcttgatgc acaagatgag cacgaacgcc agctgcggtt
    40201 actcagccaa cgacatgaat tacaagggca gattgccgcg cataatcagc aaattatcca
    40261 gtatcaacag caaattgaac aacgccagca gcagctctta acggcattgg cgggttatgc
    40321 actgacattg ccacaggaag atgaagaaga gagctggttg gcgacacgtc agcaagaagc
    40381 gcagagctgg cagcaacgcc agaacgaatt aaccgcgctg caaaaccgta ttcagcagct
    40441 gacgccgatt ctggaaacgt tgccgcaaag tgatgatctc ccgcacagcg aagaaactgt
    40501 ggcgctggat aactggcggc aggttcatga acaatgtctc gcattacaca gccagcagca
    40561 gacgttacag caacaggatg ttctggcggc gcaaagtctg caaaaagccc aggcgcagtt
    40621 tgacaccgcg ctacaggcca gcgtctttga cgatcagcag gcgttccttg cggcgctaat
    40681 ggatgaacaa acactaacgc agctggaaca gctcaagcag aatctggaaa accagcgccg
    40741 tcaggcgcaa acgctggtca ctcagacagc agaaacgctg gcacagcatc aacaacaccg
    40801 acctgacggg ttggctctca ctgtgacggt ggagcagatt cagcaagagt tagcgcaaac
    40861 tcaccaaaag ttgcgtgaaa acaccacgag tcaaggcgag attcgccagc agctgaagca
    40921 ggatgcagat aaccgtcagc aacaacaaac cttaatgcag caaattgctc aaatgacgca
    40981 gcaggttgag gactggggat atctgaattc gctaataggt tccaaagagg gcgataaatt
    41041 ccgcaagttt gcccaggggc tgacgctgga taatttagtc catctcgcta atcagcaact
    41101 tacccggctg cacgggcgct atctgttaca gcgcaaagcc agcgaggcgc tggaagtcga
    41161 ggttgttgat acctggcagg cagatgcggt acgcgatacc cgtacccttt ccggcggcga
    41221 aagtttcctc gttagtctgg cgctggcgct ggcgctttcg gatctggtca gccataaaac
    41281 acgtattgac tcgctgttcc ttgatgaagg ttttggcacg ctggatagcg aaacgctgga
    41341 taccgccctt gatgcgctgg atgccctgaa cgccagtggc aaaaccatcg gtgtgattag
    41401 ccacgtagaa gcgatgaaag agcgtattcc ggtgcagatc aaagtgaaaa agatcaacgg
    41461 cctgggctac agcaaactgg aaagtacgtt tgcagtgaaa taactattca gcaggataat
    41521 gaatacagag gggcgaatta tctcttggcc ttgctggtcg ttatcctgca agctatcact
    41581 ttattggcta cggtgattgg tagccgttct ggtagttgtg atggtggtat gaaaaaagtc
    41641 attttatctt tggctctggg cacgtttggt ttggggatgg ccgaatttgg cattatgggc
    41701 gtgctcacgg agctggcgca taacgtagga atttcgattc ctgctgccgg gcatatgatc
    41761 tcgtattatg cgctgggagt ggtggtcggt gcgccaatca tcgcactctt ttccagccgc
    41821 tactcactca aacatatctt gttgtttctg gtggcgttgt gcgtcattgg caacgccatg
    41881 ttcacgctct cttcgtctta cctgatgctt gccattggtc gcctggtttc cggctttccg
    41941 catggcgcat tttttggcgt cggcgcgatc gtgttatcaa aaattatcaa acccggaaaa
    42001 gtcaccgccg ccgtggcggg gatggtttcc gggatgacag tcgccaattt gctgggcatt
    42061 ccgctgggaa cgtatttaag tcaggaattt agctggcgtt acaccttttt attgatcgct
    42121 gtttttaata ttgcggtgat ggcatcggtc tatttttggg tgccggatat tcgcgacgag
    42181 gcgaaaggaa agctgcgcga acaatttcac tttttacgca gcccggcccc gtggttaatt
    42241 ttcgccgcca cgatgtttgg caacgcaggt gtgtttgcct ggttcagcta cgtaaagcca
    42301 tacatgatgt ttatttccgg tttttcggaa acggcgatga cctttattat gatgttagtt
    42361 gggctaggga tggtgttggg aaatatgcta agtggcagga tttcaggacg ttattcacca
    42421 ctgcgcattg cagcagtgac tgactttata attgtactgg cactgctaat gctctttttc
    42481 tgcggcggca tgaaaacaac gtcgcttatt tttgctttta tttgttgcgc gggattattt
    42541 gccctttcag cacctctgca aatattatta ctacaaaatg ccaaaggcgg agagttatta
    42601 ggtgccgcag gtgggcaaat agcgtttaac cttggtagcg ccgtcggcgc atattgcggt
    42661 ggtatgatgc tgacgctggg gctggcatat aattacgtgg cgctgcctgc cgccctgctt
    42721 tcgtttgctg cgatgtcgtc attgctgctg tatggtcgct ataagcgcca gcaagcggcg
    42781 gatagtccgg tgctggcgaa accactgggg tagattgcag tcacgttgcg cgttgcttat
    42841 tgccggatgc ggcgtgaacg ccttatccgc cctacgcggt tccggcacat tttgtaggcc
    42901 tgataagacg cggcaagcgt cgcatcaagc atgatgcgcc aattgcctac gttttttact
    42961 cttgtggcca taaccacgca gcgccgcgta cgccgctgga atcaccgtgc ttcgccttac
    43021 gcaccggcgt ttcacattcg ccgccgaaga caaattgttt aatcaactgc ccaaccgttt
    43081 gatataaacg gtctacattg ctcatcccgc cccccaggac aatcacatcc ggatcgagaa
    43141 tattcacgac atgtgccagc gattttgcca gccgcagctc gtagcgacgc aatgccagtt
    43201 ccgctaccgg atcgctttct tcaaccaggc ggataatttc actgcctttc agcgcatgtc
    43261 cgctcaaacg acgataatca gtcgcgaatc ccgtgcccga aataaaggtt tcaatacaac
    43321 cttgtttacc gcaataacaa gggacttcct cgcgataacg cagttcgtct tcgtccatcc
    43381 acggtagcgg attgtgtccc cactcacctg ccgtgccatt gccgccgata tgcgcccgcc
    43441 cattgaatgc cacgcccgcg ccgcatcccg tgccgataat cacggcaaat actgtctgcg
    43501 ctcccgctgc cgcgccatct actgcttctg aaaccgccag acagttggcg tcatttgcca
    43561 gccgcacttc ccgctgcaac ctcgcgctta agtctttatc gaatggctga ccgttgagcc
    43621 aggttgaatt ggcattcttc accacaccgg tgtaaggcga aattgagcca ggaatgccca
    43681 tacctaccgt tccgcgctgc cctgtcgcct gctccgccat atcaaccaac gtggcgatcg
    43741 tttcaatagt ttgccggtaa tcatcacgcg gcgtgggcag acgatggcgg tacaactgct
    43801 cccctgcatc gcccagtgca atcacttcag ttttggtgcc gcctaaatcg atacctatac
    43861 gcacggtact ctccttattt ttttcaatat caatagcgta gagacggaca accggattgg
    43921 caatgcaagg ccgccgacaa ttcgttatca tgcccgctaa atttaacgac aaggccgtgg
    43981 aaattatcat gctgtggttc aaaaatttaa tggtttaccg tcttagccgc gagatttcgc
    44041 tgcgtgcaga agagatggaa aaacagctag cctcgatggc atttacccca tgcggcagcc
    44101 aggacatggc gaagatgggc tgggttcctc cgatgggatc gcacagcgat gcgttaacgc
    44161 acgttgccaa tggtcaaatt gttatctgcg cgcgcaaaga agaaaaaatc ctcccgtctc
    44221 cggtgattaa acaggcgctt gaagcgaaaa tcgccaaact ggaagcggaa caggcgcgta
    44281 agctgaagaa aaccgaaaaa gattcgctga aagacgaagt gctgcactct ctgctgccgc
    44341 gtgctttcag ccgttttagc cagacaatga tgtggatcga cacggttaac ggtttgatta
    44401 tggtggactg cgccagtgcc aaaaaagcgg aagatacgct ggcattactg cgtaaaagcc
    44461 tggggtcgtt accggttgta ccattgagca tggaaaaccc gattgaactg acgctgaccg
    44521 aatgggttcg ctccggtagt gcggcacagg gcttccagct gcttgatgaa gccgagctga
    44581 aatcgttgct ggaagatggc ggcgtgatcc gcgcgaagaa acaagatctg accagcgaag
    44641 agatcaccaa tcacattgaa gccggaaaag tggtgactaa actggcgctc gactggcagc
    44701 agcgcattca gtttgtgatg tgcgacgatg gttcgcttaa gcgtctgaag ttctgcgacg
    44761 agctgcgcga tcaaaacgaa gatatcgacc gtgaagattt cgcccagcgt tttgatgccg
    44821 atttcatcct gatgactggt gaactggcag cgttaattca aaacctgatt gaaggattag
    44881 gtggcgaagc acaacgttaa ttgctgattt tcctttaatg ccggatgcga cgcctgccgc
    44941 gtcttatccg gcgtacgaag ccacaccagg catataatta ttcgctacgg cgggcaataa
    45001 tttttagcgc agcaatatta tgcgttttat gctgtaactt gctccatgga cgttgtgtca
    45061 ttgtttttcc tcaagccagt gacgcgcttc actgcgaatt ttccgcattg ccccggtttt
    45121 cactaaatcc cgtaacagcg aaagatcggg ttgttcgaca aataagagta atgatggatc
    45181 ctcttttaac cacgtcgttt tcacatcggc agcgagctgt ggattattca tagctagcga
    45241 ccgatgccga gactcggtca atgtcgttaa caacgacgcc ggggtatgtg cgttgcgtaa
    45301 tgccgtttgg cgcaaatgat cattgcgact ttgcagcaat gcacgccacc attcatcatc
    45361 tgatttatct tttatcgtag ccagttccgc aacaatatct ggagatcttc actgatattt
    45421 atatcaacct tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag caaaggaaat
    45481 attattatgg gttaaagaga ggatatttca gaaaaatgcc ctcccgttca aggggaaggc
    45541 gaggaattac agatagcggc acagataaga ggtgggttcg gcaacttgca gatgaaactc
    45601 actgtgaccg ggaacattaa acaccgaacc ggcttcatac acctgccagt cggtcgcgtc
    45661 aggcagtaac acattcagcg cgccactgat taccgtcatc tcttccggct cagcggtgct
    45721 gaaggtgtat tcgccttcaa ccataacacc cacgctggcg cgaccagtgc tgctgctgga
    45781 aaagccgatt gatttcactt tgccggaaaa gtactcatta ctttgaagca taaactggcc
    45841 ctgtctggta gttgaacatc tacttcacta taggggccag aggcgctggc tgtcacgcaa
    45901 aattacacca gtaattccgc agccagccgt gcaatcaata cgttagacag caagacagga
    45961 acatcgagct gtttttgcag taaatcgcga tgacgttggt gaaatcctaa acaatccagc
    46021 atgatgacat cagccccttt tgccagtaat tctttcccgg catcaatgat tttttgttct
    46081 gaatcatgaa tggggttgcc caatgaaaat accggcgatt tctgcaaaat ttgccatttt
    46141 tgcgcctgaa cggtcagcat ctcctcaacc ggaacgataa cccccacctg atgatcttca
    46201 acaatagagg aaaccagtgg aggcaatatt cgcgacggct caagaaagat cgtattacgc
    46261 gcagtcatac tactaatgtt tgctgtactc attaataaaa tgacgtcata accctgatta
    46321 tcgagcactt caaccacacc ttgcaggtct cgctccactt tacgacgcga aacatgggcc
    46381 agctggttgt catttaataa tgtgagaatg gtgtcttcgc ctgcttctgg cgcgtactcc
    46441 gccatcactt cttcacgact taacttcccc agcaagctat gatgggaaat attatcttcg
    46501 tcaatgtatt ccgtcaggag cggcaaaacc tcctgcatag gtacaatgcc gatggtcagg
    46561 atcgccaacg acgcactcat tgttcatcca ccttactttt ctttcgtcgt taattaccgg
    46621 gcaagtgtga agcaccactg ctgacactta cttgtgctac aaatgacaaa aagcgtagca
    46681 gcagacgtgc acggcataat gtaaagattc caaatgattc cagtaatgga ttgttattgt
    46741 ttaatattct aattattaga aaaacatgaa ttatgaaaaa tgtgacgcag atcccttgta
    46801 gtggcactca gaatcccttc cgagcagtct gatgcggtca ctctttgtca gggtcctcat
    46861 agcgtttttt cgcatccatc gcttcctgag ccgtcggatg ttcactgatc aacgactctg
    46921 gtttaggatg atcggctctg agttggtacc aggttaccgt ctgtcctggc tttccttttt
    46981 caatcgtcac tatatatgct tcacgaggat aaggcggttt cgttggcatc gttcttcctt
    47041 atttcacggg atgaacgtta agtataggcg ctctaaaatc aacaattgat cgtctgtgcc
    47101 agggcgctac gaatttcaga aatcacctgg ctgggttcgt ttgttgcgtc gatgataata
    47161 tgcgcaactt cgcgatatag cgcatcgcgt tcttccagca cttcctgaac ttcttcgctc
    47221 agcggttttc ccgttaaggt tggccgtaaa tcttcttccg gtgcagcttg cagtcggtta
    47281 accaggactg atactggcgc acacaaataa accacgatcc cgttattttg catgaagtga
    47341 cgattaaatt ccgtcagaat aatgccgccg cctgtagcga taacggtgga tggcgcagtt
    47401 accgcttcca gcgccgccgt ttctctggcg cgaaatcccg cccactcttc cctttcgacg
    47461 atctccgcga ccgtcatatt gagctgtgat tgcaaccact gatcggtatc gacaaaccga
    47521 cggttaagcg aatcggcaag ggccattccg accgttgttt taccacagcc ccgaggcccg
    47581 atcagaaaaa gaggttgtgt catcgtgggt tttccccaat aggtcgcaat gcggcgaaag
    47641 ccggtgtcat gagaatagcg atcataccat caaactagta caatttcgat tgtaaagaaa
    47701 aaattccact taaagtgaaa atctcaatac atcccttact ataccaataa atgttcaaga
    47761 atgaagtgta aataataaat tacatttagc cacgactacg ttgcacttcc agccaccact
    47821 tctccagctc cgcggcaaag gcctggcggt cacgttgtga aaggctatct ggtccgccgg
    47881 tctggatccc actggcacgt aaggtatcca taaaatcgcg catcgtcagg cgctcacgaa
    47941 tggtcgctgg cgtgtaacgt tcgccgcgcg gattaagcgc cgctgcgcct ttctcgatgg
    48001 cttcagcagc caaaggtata tctgcggtga tcaccaaatc gcccgcttca cactgccgga
    48061 caatttcgtt atcggcaacg tcgaaacctg ccgcgacgcg cagcgtacga ataaatcgcg
    48121 atggcggcac gcgtaaactc tggtttgcta ccagtaccag cggcatctgc atacgttccg
    48181 ccgcgcgata caaaatctct ttaattacat tgggacacgc gtcggcatcc acccaaattg
    48241 tcataaagtc atcctttgtt gggtaatcct ctattgtgtc gcgcttttgc cttccggcat
    48301 agttctgttt atgcttctgc cagcgattat caaaacaatg aatttcacgg caggagtgag
    48361 gcaatggaaa agaaaatcgg ttttattggc tgcggcaata tgggaaaagc cattctcggc
    48421 ggtctgattg ccagcggtca ggtgcttcca gggcaaatct gggtatacac cccctccccg
    48481 gataaagtcg ccgccctgca tgaccagttc ggcatcaacg ccgcagaatc ggcgcaagaa
    48541 gtggcgcaaa tcgccgacat catttttgcc gccgttaaac ctggcatcat gattaaagtg
    48601 cttagcgaaa tcacctccag cctgaataaa gactctctgg tcgtttctat tgctgcaggt
    48661 gtcacgctcg accagcttgc ccgcgcgctg ggccatgacc ggaaaattat tcgcgccatg
    48721 ccgaacactc ccgcgctggt taatgccggg atgacctccg taacgccaaa cgcgctggta
    48781 accccagaag ataccgctga tgtgctgaat attttccgct gctttggcga agcggaagta
    48841 attgctgagc cgatgatcca cccggtggtc ggtgtgagcg gttcttcgcc agcctacgta
    48901 tttatgttta tcgaagcgat ggccgacgcc gccgtgctgg gcgggatgcc acgcgcccag
    48961 gcgtataaat ttgccgctca ggcggtaatg ggttccgcaa aaatggtgct ggaaacggga
    49021 gaacatccgg gggcactgaa agatatggtc tgctcaccgg gaggcaccac cattgaagcg
    49081 gtacgcgtac tggaagagaa aggcttccgt gccgcagtga tcgaagcgat gacgaagtgt
    49141 atggaaaaat cagaaaaact cagcaaatcc tgatgacttt cgccggacgt caggccgcca
    49201 cttcggtgcg gttacgtccg gctttctttg ctttgtaaag cgccaaatct gccgatttca
    49261 accactcacg atagtgactc atttgtgggt tcagcggcgc aacccccaca ctaatccgta
    49321 aagttacctg tggcgtattc ggcaagcgta acgtgtttag cccttcacgc acccgtaaca
    49381 tggcggtaat ggcgctctca gctggcgtac cggacatgat tactgcaaac tcatcgccgc
    49441 caaaccgacc aatcacatcg ctaccgcgca gggtaatttg taactgtcgg gtaagcgcca
    49501 caatcgcttc atcgcccaca tcatgacccc aggtatcgtt gatgctcttg aaatggtcga
    49561 tatcgataat cagtaacgtt gcatcgcgat tatgccgccg acagttatca aattcattgc
    49621 gtaacatagt ttcccaatga cgtcggttat acacgcccgt catgccgtcg cgggtactca
    49681 tgacctgcaa cctgcgttta tgttccgcca gtttggttgc cgtctggtag ctgacccagc
    49741 caaacagcag aggataaacg acaataatgg gaagggagag ccaccattcc agcggcgcac
    49801 tattgaacga cacggtaatg cccgtaagct cgagggtgac aaggcaggaa accaccatca
    49861 acaccagacc cgcgacaaac agacgggggc cgcctgcccc catcaaattc agacacataa
    49921 tcatcaacat agcggtggaa ggcagcacgt ttacgcccat tacgcctacc cacattcccg
    49981 ctaatactgc atcggttttt aagttgtaaa tttcccggct aagcggatcg acggccctgc
    50041 tcgctatctg ccaggctaaa tgcggccaga cgaacgccca gccgaccaac accagccacc
    50101 accagcccgg cggcgggtgc gaaaccagcg ttgaagcaat cggtaagaac atgccagcca
    50161 ggccaaccgc acggggtagt ctgacgcgac gggcgaagcg cagcccggac cgctgatgtt
    50221 cgttttgagg agttaaagga ggttcctccc catgcgccgc cgcttttttg aaaaagtttt
    50281 catcattcat tatttttggg aacattcaga aacaattttc ccaaattata gagacggggc
    50341 taaaggcaga agtatgaaat ttcgggtgag ccactttcgt agctcacccg ttagcactat
    50401 caggtatcag gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt
    50461 cagagattgt tgcgttgctt gattattaca ttcgcgcatc ttttgctgct gtggcgtcaa
    50521 acttttttca ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg
    50581 agcatcccct ttcaacgcct gcgccgtcgc ctgctgatta caggatgtca tacgctgttg
    50641 ttgtggggtt aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag
    50701 caaggtaacc agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc
    50761 gtatgcgccc gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag
    50821 cgaaaattca ctgccgggcg cggttttatt tcagccccag ggcggctttc atggtgtaga
    50881 agagatcggt ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa
    50941 tacgcaactg actgccggta tgttcttgtg aatcctcttc ggagttcccg tagctcatca
    51001 ccatcactgc gccatctttg gtatttagcg cctgggtgag gcccggagct ttggtatccg
    51061 gcgcgacaat ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtattgccat
    51121 cctttttagc gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa
    51181 tttgcccaca aggattcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca
    51241 ggaaaaatcc tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgcgcca
    51301 gggttggtac actgtcatta cgttgcggat taggcgtaca ggtgactgcg ggcttatcga
    51361 tattgccgtg gtacgttgct ttcggtccta gccagcgcac tggcatattg ccgtcagcaa
    51421 acagtccaag cagaggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat
    51481 cgctgaccaa ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt
    51541 caccggcggt tgccgtttca gcaaaggttt ttgcgccacc gccaagcgta acgtcggcac
    51601 gagcgttaag cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg
    51661 gacatttttc actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca
    51721 gcgcagcggg cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg
    51781 cggcttttgc catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg
    51841 cgccgttata ggttttgaca ccggttgacc aggcggttgc tgatgcagcc gaatcggtga
    51901 cgtagtccgg tttgccggtt tttttattca gcgcatagtg agtgtattgc ccggtaagcg
    51961 gtaaggcatc gataccttta aaaaagccgc ccgcaccttc ggcataatta cgtgcggctg
    52021 taatttccga atcccccatc ccatcgccaa tcagcaaaat aatatttttt gcaggtttat
    52081 cgctaagaga atcacgcaga gcggcagtct gatcacccgt taaacggcga gcaccgccgg
    52141 gtgcagtaat atcgccctga gcagcccggt tttccagaac aggcatttct ggtgtccggg
    52201 cttttgtcac aggggtaaac agtaacgata agagtgccag tgcaatagtg ctttgtttca
    52261 ctttattttc tccatgtaca aatacattaa aaaataaaaa caaagcgact ataagtctcg
    52321 gccgtgacaa ctttatgaca gctgttgaaa agattaactt tttactgacg aggatgcttc
    52381 aataacttct ttacgtaatc gcgcagcagc tccgtatcgt cgtcaggaat gctggcatcg
    52441 ggctttacct cgtacagcgc cccctctacc tgatcaatca accgctgttg gtcattttgc
    52501 gccatattgc gaagcattgc agtgacgata atctccaaag cttctacctg cgcacacagt
    52561 tctttcgact cttcttcttt ttgggcaagc ttaaataaca actcagcaat gagatttttc
    52621 atgtctgtat ttccttatcc aaagtatgga gaagttaaca ttatgtttca caacttcata
    52681 gtgtctggaa gtattatttt tatgacaaag gaaatacttt gcgcaaatat tttttgtttg
    52741 attaccagcg aaacgtttct cttatttaaa taattaccca cacaaaatat aggcttttaa
    52801 ttatcaacct ttattataaa taaaagttat gaaatatatt ctcttcgtta acttataatt
    52861 aagagaaaaa aactcaccgc atggccattt aagccaggaa taaattagct tcccgttcat
    52921 catttttgtg atcttgcgca catctttgag ataacgcact ttcttcacca gtacgttgca
    52981 cgcttcgtca gcagaagatt gcaacaatct ttcagaaggc cgcgaatccc ccgttttgtc
    53041 gtggtaatgt atgcactttg tgttagacgg gataggtttt taagatggaa aaactgcggg
    53101 taggaatcgt ttttggtggt aaatcagcgg aacatgaagt gtctctgcaa tcggcaaaaa
    53161 acattgtcga tgccattgat aaaagtcgct tcgacgttgt gctgctgggc attgataaac
    53221 aagggcaatg gcacgtcagc gatgccagca attatctgct aaatgcagac gatcctgccc
    53281 atattgcgtt acgcccttcg gcgaccagcc ttgcgcaggt gccaggtaaa catgagcatc
    53341 agcttatcga cgcgcaaaac ggtcagccgt tgctgacggt ggatgtcatt ttcccgattg
    53401 tccacggtac gctgggcgaa gatggttcct tgcagggaat gctgcgggtc gccaatttac
    53461 cgtttgtagg ttctgatgtt ctggcttcag cagcctgtat ggataaagat gtcaccaaac
    53521 gtctgctacg cgatgccggg ctgaacattg cgccatttat tactctgacg cgcgctaatc
    53581 gtcacaacat cagttttgcc gaagtggagt ctaaactggg gttaccactg tttgtaaaac
    53641 cggctaatca gggctcttct gttggtgtca gcaaagtaac cagtgaagaa cagtacgcaa
    53701 ttgccgtcga tctggcgttc gagttcgatc ataaagtgat cgttgagcaa gggatcaaag
    53761 gtcgtgagat cgaatgcgca gttctgggca acgacaatcc gcaagccagc acctgtggcg
    53821 agatcgtact caccagcgat ttctatgcct acgacaccaa gtacattgac gaagatggcg
    53881 cgaaagtggt agttccggca gccattgcac cagagatcaa cgataagatc cgggcgattg
    53941 ccgttcaggc ttatcagacg ttgggatgcg caggcatggc gcgtgtagac gtgtttttaa
    54001 ccccagagaa cgaagtggtg atcaacgaga tcaacacact gcctggcttc accaacatca
    54061 gtatgtatcc gaagctgtgg caagccagcg gtctgggtta caccgatctg atcacacgtt
    54121 tgattgagct ggcgctggag cgtcacgctg cggataacgc attgaaaacc acaatgtaat
    54181 attttaacgc ccgataatat tcttattggg cgttttcagt ttcttcatcg cgacgacgaa
    54241 taatcaaccc cgccagccag aaactaatca cccacgtcac cagaccgacg gcataggtct
    54301 gccaaccttt tgcttcaaat cccagtagcc ccaccacgcc attaagaatg aatataaggc
    54361 caatggcgaa cgcatagtag tgccagtcac ggcggatttt tacaggcaga ttcatggcag
    54421 ctccgttaac gaaaaacaac atccttgcat attctgtaac aaccgtctgt ggtgtagaca
    54481 acatttttgt aattgttaat aaatttaact taaggttgcc attttgtgac tcacaacgaa
    54541 gattcacgct tcgggaatat tcccaacatc aatttaccat gattctgata ttgacaatct
    54601 aaatgacctg tcagacttgc cataagccgc tggtatcaca ccagaagaga agcagatgac
    54661 ttccggaggt ttttatggct gatttcaccc tgtcaaaatc gctgtttagc ggaaaatatc
    54721 gcaatgcctc ttcaacgcct ggcaacattg cctatgcgtt gtttgtgctg ttttgctttt
    54781 gggctggggc gcaattgctg aacctgttag tgcatgcgcc cggcgtctat gagcgtttaa
    54841 tgcaggtcca ggaaacaggt cgcccacggg tggaaattgg tttaggtgtc ggcaccattt
    54901 tcgggctgat cccgttttta gtaggctgcc tcatttttgc agtggtggcg ctatggctgc
    54961 actggcgaca tcgccgccag taagatttac gttgccagat acggcctaaa acgccttatc
    55021 tggcatgaca ttcctgtttg atcactgttt catacaacgc gcccgccgtt cgtctacgcg
    55081 cttcgcaaac caggccgtag tcaggttgcg ggtgattttc gggctttcca gttgaatgcc
    55141 aggcaacatt tcgcggggta gtgatttacc cgttttcgct tcggcaagtt gataaacttt
    55201 cttgtacagc gccgtctcct caaaagagaa gctgtcacct ttctctaact gacggcgaat
    55261 ttcgctgtcg ctcatcccca gttttcccgc cagtttgcgc gttgccagtt cggttttccc
    55321 gggttcttta ctgtcgtaac gaattaaatc accgtccagt gccagcttca cgccgctggc
    55381 cttactgact gcgttctgaa acgcggcatt acggctggcg taccagccag cgttaaaatc
    55441 agcaaagcgg tatatcggtg cgctatagct ggcgggataa ttcagtaaat ggtaagtacc
    55501 aaaccacaac ccgccgcggc gactgaagac ttcctgacga actgtaccgt ccattttcca
    55561 cggataccct ttggtatgct gttcggcaaa agcaatgctg acctgcatcg gcccaccggt
    55621 gcgcaccgga ttaagcgaac caaacagcgt ctgccccatt ggtaccatgc tgatcagatc
    55681 gtcaaaaatc gcgctcaatt gcttttctgt acgcaccgaa tctaaccgtt cgctataact
    55741 tttgccgttt ggcgatttga tttttagcgc ggtatgaacc agaaaagcag gaatgtgcat
    55801 ccgttcagca cgacggtcaa tctcttgcca ggcgatcttg cttaagcccg gaacggccgg
    55861 atctgcctga taatttgact cctgctgcgc caccgccagc accgagcaga cgttttccag
    55921 cgtcggtgcc agcccctgac tttcaaaggt ggtggcgaga tctttcgccc aggcatcgcg
    55981 gtctttcacg ctcgcaggca tcttctgccg cacgacgctc gctacatcta tcgctttttc
    56041 gccctttttc aacggttgcg gagcctgact actacacccc gctaaaacca gaaccgccaa
    56101 caatgacagc gaagatagat gattgacgcg cgacattact tctccttttt agctcaaggt
    56161 atgggttact tcctgaattt tgtcaccatc cagctcatgt tcaaagctgc gcagacgttt
    56221 gtagatagac atcaactcaa ccagtgtggt ccatgagtta atcaggtact ggaaagcacc
    56281 gcgaacctga ccaaaaacgt tggtaatctg cgtcatcagg ccgagcgtaa tcgtaccggc
    56341 aacaattgac ggaaacagca agaacaaacc gaaaacgcta tcgacctgca aatagagaat
    56401 gcgggcgatg ttgaaataca tatagtgaaa atagaggcga aaatagtttt tccgtacggc
    56461 gctaaacagc tcgcgtaccg taggcggcgt cgcgcgcgtg gcatcgtctt caccataaac
    56521 cagctcttta cggtaggcgg cctctactcg ctggttttta aactccagcc ccggcagttt
    56581 gatccctacc actgccagca atccggtccc catcagcgac cagacgatag cggcaatcac
    56641 cagaccatac ggaatgtgcc cgacaattgg cagctccggc acatgtgcgg agagcgttac
    56701 cagcaccggc aggaaggcga tcaacgtcat gatggcgttg ataaaactga cgcccatatt
    56761 ctccagcgtt gaagcaaaac gcatggtgtc ttcctgcaca cgctgtgcgg ccccttcgat
    56821 atgacgcagt tgttgccagt tcgccatgta atattcgttc atcgccgtac gccagcggaa
    56881 cacgtagtga ctgacaaaga agttgttcag tacactgatc accaccgcga tcagcgcaat
    56941 ccccagaaag acgcccactt cgcggtaaaa ttgttcgata gtgactttat gcggcgaact
    57001 tagcgcggtt tgaatcagat catagaacgg tgcataccag gcgttgacgg cgaccccgac
    57061 ttccaccaaa aaccaggtga cgaagatgat cagtgcagta ccgagtattg accagtattg
    57121 ccaacggtgc gggctgtaga taaaccagaa aaatgcaaaa agtcctacgc aaacaatgta
    57181 gtaagcgtaa aaaatcagga aatccaacga ccagaaacgc gcggcgctaa tcgggatctg
    57241 cccggaagcg ccggtgatac gcgccaccca gtctccccca ccggcttgcc agaagataac
    57301 ggcaatcaat gcccaaacaa aggccgagag aaaaaacgct cccggctttg ggaaaaaaga
    57361 cttaaacata tgcactcctg ctaacttctt atcgttttaa caactgctgt gtattaaccg
    57421 catgaccact acaaagcgtc agccaaatga aaccgcccat tgtgacccat agcaaggcgc
    57481 ttagttcgcc agcctgacca taagaattgt ttcgacggat ttcacaaacc aaagattgtt
    57541 tacataagcg gattaacacg cgcaggatca caaatgcagg cattggtgag caggaaaaag
    57601 atcgtgttta cgcgccgtta cgtttttaac actattattg cactaattcg ccctttgcaa
    57661 tccatcaatg agtagtataa atacgctcag ttaccttcat tcaatctatg gacaccaccg
    57721 ttgaaacgta gtctgctttt ttctgccgtg ctgtgtgcgg cgtcattgac ctccgtccat
    57781 gcggcgcagc cgatcactga accggagttt gcctctgata ttgtcgatcg ttatgccgat
    57841 catatttttt acggcagcgg tgccacgggg atggcgctgg ttgttatcga cggtaaccag
    57901 cgcgtctttc gtagttatgg cgaaacgcga cctggtaata acgttcgccc gcagctggat
    57961 tcggtcgtgc gtattgcttc cctcaccaaa ctaatgacca gtgaaatgct ggtgaaattg
    58021 ctcgaccagg ggaccgtaaa gttaaacgat ccgctaagta aatatgctcc gccaggcgca
    58081 cgcgtgccga cctacaatgg gacgcccatt accctagtga atctggcaac ccataccagc
    58141 gccctgcccc gtgaacagcc cggtggcgcg gcacatcgtc cggtgtttgt ctggccaacg
    58201 cgcgaacaac gctggaaata cctttctacg gcgaagctaa aagccgcgcc aggcagtcag
    58261 gcggcgtact ctaaccttgc gttcgacctg ctggcagatg cactggcgaa tgcgtccggc
    58321 aagccttata cccagttgtt tgaagagcag attacccgtc cattggggat gaaagacacc
    58381 acctacaccc cctcaccgga tcagtgccgc cgtttaatgg ttgctgagcg cggtgccagt
    58441 ccgtgcaata acacgctggc ggcaattggc agcggcggcg tctattccac gcctggcgat
    58501 atgatgcgct ggatgcagca gtatttgtca tctgattttt atcagcgcag taaccaggca
    58561 gatcgcatgc agacgttgat ttatcagcgc gcgcagttta cgaaagtgat tggcatggat
    58621 gtccccggca aagcggatgc gcttggttta ggctgggtat atatggcccc gaaagagggt
    58681 cgtccgggga ttattcagaa gacaggaggt ggtggcggtt tcattaccta tatggcgatg
    58741 atcccgcaga agaatattgg cgcgtttgtg gtggtcactc gttcgccgtt gacgcgcttt
    58801 aaaaatatga gcgatggcat caatgatctg gtgaccgagc taagcgggaa taaaccgttg
    58861 gttatccccg catcctgatt aatactctga aggcaaacgg ttgatagcga ccagtttgcc
    58921 tttattcatt tcgatatagc cgcctttacg caaagcagcg agaacttctg cgacgacaga
    58981 acgcgatatg cgtgtacgtt gatggatata attcatcaca ccaatacgtg atcgcaattc
    59041 ttcattccag tcaatcattg ataataatgt tgcgcggatt tgttcgtagg aattatgccc
    59101 aatgagctgc acgtcgcgta attccagaat tctattttgc caggctaacc agtaaaaagc
    59161 gtctcgccag agttgatttt gttcaataag cgtaatggtt tgtttggctg gtagatgata
    59221 tcccgtacaa tttccttctg atattaattt gtatggtata tcgtttttca ttaaaccatc
    59281 agccagcccc ataatataag gagcctgggt aataccgata agtacgtttt cttctctacg
    59341 cagagagata acgccctcta aaataacgaa agtatcttcg ctattgacat cactggaaaa
    59401 tataacttgc ttttcattat taaactcgaa gcgcgtaccg tatctggaca aacatttatc
    59461 gagcttacca aattcctgaa gaggtttaac tacagataac atttgcgcgt cctttgcagt
    59521 aatgcccgtc aaatccttga cgggcattat ttagattaaa ttaccagaat ttcttcggag
    59581 tgaagaatat taccaggtat atttaacacc cacgttcgcg gaccagtctt gatctacgtc
    59641 accaccaccg aggtagttag catcggtata ggcgctgaag ttcttggtga agctgaactg
    59701 agtacccaga ccaacacgta ccgcagaccc ttcagtaccg ttatcgatgg aatcaccgtt
    59761 cacatcgtta tcgttgttag agtcgtcgta gacgtaagcc agtttgaagt acggagtcag
    59821 agcctggtct tcgctgtagg tgaaggtata acctgcatct acacccagtt cataacgcat
    59881 gctgtcgtaa gactgaccgt caactttcat gtcgttgctc agctggtagt catcaccaga
    59941 ctggaacaga ccagaaatgc tgccgtaagg agtcacgtaa ccagcatcac ccagtttgaa
    60001 gtcgtaaccg gctttcaaac cgaagcccca agcgtcggag ttggtgctac cgtcaacgta
    60061 agtaccgttg ctcatggttg cagacaggtc gttgttgaag tgagagtaac tcaagctacc
    60121 atcaacaaag acgttgttcg cgaagtgagc agaagagtag atgtaggcag tctggctgtc
    60181 ttgatccacc tgaccagaac ggtcattcat gtcaccttta gcgaagcctg cagccgcacc
    60241 gacgatccac ttagcgttgt taccgtcaat tttggtatca acaccgacca tgatgccgtt
    60301 aacatcctga tcatagttga tggtgccgtt gtcgccgttg aagttaccac cgaagtagct
    60361 tacccatgcg ccgccgttat cagccaggcc atgacgagag ttggtcagac gagtaccaac
    60421 ggtgtcttgt tccaggttcc agatattggt gttcgcagac gggatgctca gcgccatgtt
    60481 agcgtagtcg gtcagctcca tctgttgcag aacaacggtg ttaccgcgct gttcagcctg
    60541 ataggtgtat gcacccaggt cagctttgtt agcagcagag aaggtcgcgt tgctgttttt
    60601 gtcgttgacg tagatcagct cgttgccgtt gtagtcagcg atagaaccag caccagtagc
    60661 gttgtcgata cgaactttgt agttaccggt tgcagctgca acatggtcac cgttgttatc
    60721 aacttcgttc tgaccagctt cgatagaagc agtgttgtcg ttatcgccgt taccgttaat
    60781 ggtcaggtga ccatcagagt tcattgcgat taagccataa ccgtagtttg acttggtagt
    60841 atcgttggta cggtcgttgg tcaggtctgc atcaagtacg aagcggttgc tttcgatgtt
    60901 gaacacacca gcgtgaatgt taccactggt atcagtagtg ctggtcaggt tcagagtatc
    60961 aacctgcaga gcattcgccg cagtgttacc gacatccaga ataccgtcat tagtcacttt
    61021 gatgttgttg gcatacagag cagcatcacc tacagcccaa ccagcagaaa cttcatcaga
    61081 aatggttacg gtactgttgt cgacagtcag tgaatcagtt gcaacatgac catcttcgtt
    61141 gatgttcagc gcagaaagac ctgtcagaga gatagagtca gaaatcagcg ttgaatcagc
    61201 aacattaaca gttgaaccac tgttgatgct cagagtatca atcagagaag ttttggtggt
    61261 atcccactgt gaaccggtat tcagggtcac attgaacaga ccgctctggt atacttcgtt
    61321 accgatgatg tgatcaaatt cgctgtattc agagtcgctg ttttctacac caacagttga
    61381 tgaaggccac aggctgttag ctgcgatgtc gatcagagtt gcagttgctt cggtgcctgt
    61441 actggtatca ataacaaccc cgttgtcatt gtaaacagga ccgtaagcat ctggcacacc
    61501 atcatcattg gtatcaacag ttaagtgcgc agacattgcc gcaccaaccc acttgctgcc
    61561 attgttcagg gtcagatcca ggcgatcagt accatcccaa ccgttagtat ctacatcacc
    61621 gtcagcatca cgatagctat cacgaccatg cgggaagaag ttctcatcaa agttacttga
    61681 gaagaataca tcgcccatca gtgtagaatt atcgaatgtt gcagtggttt gcattgcgtt
    61741 atcagcagca ggatgtgcaa taacggctaa tgcgatatca tctctattcc agtcaccgcc
    61801 attgtaatcg ctggcattgc cggtattacc aaaccaacca ctagtacctt catcagacca
    61861 tgaaccagag gtcacagtag agtctttaac agtgatggtg ttgttaaaca cttcgctgct
    61921 gttaacacca gtgctaaccg tgttgtcgtc gatatcttcc cactcataac cctgagtcaa
    61981 cgtaataccc gcaacgtgag agttattagt gatggacaga tcaacttcct gatccagagt
    62041 aatagccgta ccaatgttgt aaacgtctac aacatgagta tcagcggcat tattgtaagt
    62101 gccgttatat gtgtagtgtt cgtagttgtc gtcgatcgtt gaattatcaa cagtcagcgc
    62161 cagacggtca tggtaataat cattcgcacg ttcagcacaa tcagttgtca tgcactcgga
    62221 ataaatcata ccgtgaatag tgctgttttt gattgtcaga ctattggcgt tggtattggt
    62281 agaaataccg tcgtccagat agtaggtaga aatcacgccg ttaacagtag cgttattaat
    62341 gaccgggtag atatcgccgt tgtaataact atcagcggca tagttgttcc agcctacata
    62401 accatcatag taaacgtcat cagcataggt ggcgtcgttg tagtgatgga aagtattgta
    62461 agtttgacca gaaatatcgt tggtcgctgc attagcctga gaggtgatag ccagagtgca
    62521 agccaatgct aattgtgata ctacaagttt ctttttccag gagtgcattt gtcatccctc
    62581 ctcagggacg taataagttg atccatcaat tatcaatgca tagaaaacat attatgtttc
    62641 gcgctaacga ttatgctgcg ttacagacga atggttcaat tattctttcc cctaagtccg
    62701 aatgtggaca tatataattt atggagaaaa acacgcatca aatt
//
LOCUS       DAZGVQ010000005        62363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000005 DAZGVQ010000000
VERSION     DAZGVQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.005"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(45..1130)
                     /gene="yhfS"
                     /locus_tag="ACHLAG_000297"
     CDS             complement(45..1130)
                     /gene="yhfS"
                     /locus_tag="ACHLAG_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP0653606.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(1142..2446)
                     /gene="yhfT"
                     /locus_tag="ACHLAG_000298"
     CDS             complement(1142..2446)
                     /gene="yhfT"
                     /locus_tag="ACHLAG_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFP0653607.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(2458..2811)
                     /gene="yhfU"
                     /locus_tag="ACHLAG_000299"
     CDS             complement(2458..2811)
                     /gene="yhfU"
                     /locus_tag="ACHLAG_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFP0653608.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(2822..3700)
                     /gene="php"
                     /locus_tag="ACHLAG_000300"
     CDS             complement(2822..3700)
                     /gene="php"
                     /locus_tag="ACHLAG_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFP0653609.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(3697..4923)
                     /gene="yhfW"
                     /locus_tag="ACHLAG_000301"
     CDS             complement(3697..4923)
                     /gene="yhfW"
                     /locus_tag="ACHLAG_000301"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP0653610.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTHEVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(4923..6086)
                     /gene="yhfX"
                     /locus_tag="ACHLAG_000302"
     CDS             complement(4923..6086)
                     /gene="yhfX"
                     /locus_tag="ACHLAG_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFP0653611.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(6170..6532)
                     /gene="yhfY"
                     /locus_tag="ACHLAG_000303"
     CDS             complement(6170..6532)
                     /gene="yhfY"
                     /locus_tag="ACHLAG_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP0653612.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(6549..7454)
                     /gene="yhfZ"
                     /locus_tag="ACHLAG_000304"
     CDS             complement(6549..7454)
                     /gene="yhfZ"
                     /locus_tag="ACHLAG_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFP0653613.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(7723..8727)
                     /gene="trpS"
                     /locus_tag="ACHLAG_000305"
     CDS             complement(7723..8727)
                     /gene="trpS"
                     /locus_tag="ACHLAG_000305"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFP0653614.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(8720..9478)
                     /gene="gph"
                     /locus_tag="ACHLAG_000306"
     CDS             complement(8720..9478)
                     /gene="gph"
                     /locus_tag="ACHLAG_000306"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFP0653615.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(9471..10148)
                     /gene="rpe"
                     /locus_tag="ACHLAG_000307"
     CDS             complement(9471..10148)
                     /gene="rpe"
                     /locus_tag="ACHLAG_000307"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP0653616.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(10166..11002)
                     /gene="dam"
                     /locus_tag="ACHLAG_000308"
     CDS             complement(10166..11002)
                     /gene="dam"
                     /locus_tag="ACHLAG_000308"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP0653617.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(11109..12395)
                     /gene="damX"
                     /locus_tag="ACHLAG_000309"
     CDS             complement(11109..12395)
                     /gene="damX"
                     /locus_tag="ACHLAG_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFP0653618.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(12487..13575)
                     /gene="aroB"
                     /locus_tag="ACHLAG_000310"
     CDS             complement(12487..13575)
                     /gene="aroB"
                     /locus_tag="ACHLAG_000310"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFP0653619.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(13632..14153)
                     /gene="aroK"
                     /locus_tag="ACHLAG_000311"
     CDS             complement(13632..14153)
                     /gene="aroK"
                     /locus_tag="ACHLAG_000311"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFP0653620.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(14554..15792)
                     /gene="hofQ"
                     /locus_tag="ACHLAG_000312"
     CDS             complement(14554..15792)
                     /gene="hofQ"
                     /locus_tag="ACHLAG_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFP0653621.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(15704..16108)
                     /gene="hofP"
                     /locus_tag="ACHLAG_000313"
     CDS             complement(15704..16108)
                     /gene="hofP"
                     /locus_tag="ACHLAG_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFP0653622.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(16098..16538)
                     /gene="hofO"
                     /locus_tag="ACHLAG_000314"
     CDS             complement(16098..16538)
                     /gene="hofO"
                     /locus_tag="ACHLAG_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFP0653623.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAVWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(16522..17061)
                     /gene="hofN"
                     /locus_tag="ACHLAG_000315"
     CDS             complement(16522..17061)
                     /gene="hofN"
                     /locus_tag="ACHLAG_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFP0653624.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(17061..17840)
                     /gene="hofM"
                     /locus_tag="ACHLAG_000316"
     CDS             complement(17061..17840)
                     /gene="hofM"
                     /locus_tag="ACHLAG_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFP0653625.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPSGGDFAIALGLALGKAY"
     gene            17960..20512
                     /gene="mrcA"
                     /locus_tag="ACHLAG_000317"
     CDS             17960..20512
                     /gene="mrcA"
                     /locus_tag="ACHLAG_000317"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFP0653626.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(20677..21237)
                     /gene="nudE"
                     /locus_tag="ACHLAG_000318"
     CDS             complement(20677..21237)
                     /gene="nudE"
                     /locus_tag="ACHLAG_000318"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFP0653627.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            21557..23692
                     /gene="igaA"
                     /locus_tag="ACHLAG_000319"
     CDS             21557..23692
                     /gene="igaA"
                     /locus_tag="ACHLAG_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFP0653628.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            23757..24425
                     /gene="yrfG"
                     /locus_tag="ACHLAG_000320"
     CDS             23757..24425
                     /gene="yrfG"
                     /locus_tag="ACHLAG_000320"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFP0653629.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            24436..24837
                     /gene="hslR"
                     /locus_tag="ACHLAG_000321"
     CDS             24436..24837
                     /gene="hslR"
                     /locus_tag="ACHLAG_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFP0653630.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            24862..25740
                     /gene="hslO"
                     /locus_tag="ACHLAG_000322"
     CDS             24862..25740
                     /gene="hslO"
                     /locus_tag="ACHLAG_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFP0653631.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(25875..27599)
                     /gene="yhgE"
                     /locus_tag="ACHLAG_000323"
     CDS             complement(25875..27599)
                     /gene="yhgE"
                     /locus_tag="ACHLAG_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFP0653632.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRICNGSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            27978..29600
                     /gene="pckA"
                     /locus_tag="ACHLAG_000324"
     CDS             27978..29600
                     /gene="pckA"
                     /locus_tag="ACHLAG_000324"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFP0653633.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(29677..31029)
                     /gene="envZ"
                     /locus_tag="ACHLAG_000325"
     CDS             complement(29677..31029)
                     /gene="envZ"
                     /locus_tag="ACHLAG_000325"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFP0653634.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(31026..31745)
                     /gene="ompR"
                     /locus_tag="ACHLAG_000326"
     CDS             complement(31026..31745)
                     /gene="ompR"
                     /locus_tag="ACHLAG_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFP0653635.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            31973..32449
                     /gene="greB"
                     /locus_tag="ACHLAG_000327"
     CDS             31973..32449
                     /gene="greB"
                     /locus_tag="ACHLAG_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFP0653636.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            32546..34867
                     /gene="yhgF"
                     /locus_tag="ACHLAG_000328"
     CDS             32546..34867
                     /gene="yhgF"
                     /locus_tag="ACHLAG_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFP0653637.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            35305..35532
                     /gene="feoA"
                     /locus_tag="ACHLAG_000329"
     CDS             35305..35532
                     /gene="feoA"
                     /locus_tag="ACHLAG_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFP0653638.1"
                     /translation="MQYTPDTAWKITGFSHEISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            35549..37870
                     /gene="feoB"
                     /locus_tag="ACHLAG_000330"
     CDS             35549..37870
                     /gene="feoB"
                     /locus_tag="ACHLAG_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFP0653639.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQYEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            37870..38106
                     /gene="feoC"
                     /locus_tag="ACHLAG_000331"
     CDS             37870..38106
                     /gene="feoC"
                     /locus_tag="ACHLAG_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFP0653640.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            38309..39187
                     /gene="rpnA"
                     /locus_tag="ACHLAG_000332"
     CDS             38309..39187
                     /gene="rpnA"
                     /locus_tag="ACHLAG_000332"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFP0653641.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(39216..39986)
                     /gene="bioH"
                     /locus_tag="ACHLAG_000333"
     CDS             complement(39216..39986)
                     /gene="bioH"
                     /locus_tag="ACHLAG_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFP0653642.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWCCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            40024..40707
                     /gene="gntX"
                     /locus_tag="ACHLAG_000334"
     CDS             40024..40707
                     /gene="gntX"
                     /locus_tag="ACHLAG_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFP0653643.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIVSVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAAAV
                     QVWCLCRTL"
     gene            40766..41341
                     /gene="nfuA"
                     /locus_tag="ACHLAG_000335"
     CDS             40766..41341
                     /gene="nfuA"
                     /locus_tag="ACHLAG_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFP0653644.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            41702..43018
                     /gene="gntT"
                     /locus_tag="ACHLAG_000336"
     CDS             41702..43018
                     /gene="gntT"
                     /locus_tag="ACHLAG_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP0653645.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(43063..45147)
                     /gene="malQ"
                     /locus_tag="ACHLAG_000337"
     CDS             complement(43063..45147)
                     /gene="malQ"
                     /locus_tag="ACHLAG_000337"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFP0653646.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(45157..47550)
                     /gene="malP"
                     /locus_tag="ACHLAG_000338"
     CDS             complement(45157..47550)
                     /gene="malP"
                     /locus_tag="ACHLAG_000338"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFP0653647.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            48162..50867
                     /gene="malT"
                     /locus_tag="ACHLAG_000339"
     CDS             48162..50867
                     /gene="malT"
                     /locus_tag="ACHLAG_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFP0653648.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGMEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(50910..51926)
                     /gene="rtcA"
                     /locus_tag="ACHLAG_000340"
     CDS             complement(50910..51926)
                     /gene="rtcA"
                     /locus_tag="ACHLAG_000340"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFP0653649.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(51930..53156)
                     /gene="rtcB"
                     /locus_tag="ACHLAG_000341"
     CDS             complement(51930..53156)
                     /gene="rtcB"
                     /locus_tag="ACHLAG_000341"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFP0653650.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IEMAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            53345..54943
                     /gene="rtcR"
                     /locus_tag="ACHLAG_000342"
     CDS             53345..54943
                     /gene="rtcR"
                     /locus_tag="ACHLAG_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFP0653651.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRLQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(54925..55683)
                     /locus_tag="ACHLAG_000343"
     CDS             complement(54925..55683)
                     /locus_tag="ACHLAG_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFP0653652.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(55700..56530)
                     /gene="glpG"
                     /locus_tag="ACHLAG_000344"
     CDS             complement(55700..56530)
                     /gene="glpG"
                     /locus_tag="ACHLAG_000344"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFP0653653.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(56575..56901)
                     /gene="glpE"
                     /locus_tag="ACHLAG_000345"
     CDS             complement(56575..56901)
                     /gene="glpE"
                     /locus_tag="ACHLAG_000345"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFP0653654.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            57091..58596
                     /gene="glpD"
                     /locus_tag="ACHLAG_000346"
     CDS             57091..58596
                     /gene="glpD"
                     /locus_tag="ACHLAG_000346"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFP0653655.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(58650..59255)
                     /locus_tag="ACHLAG_000347"
     CDS             complement(58650..59255)
                     /locus_tag="ACHLAG_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0653656.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(59258..60025)
                     /locus_tag="ACHLAG_000348"
     CDS             complement(59258..60025)
                     /locus_tag="ACHLAG_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0653657.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLQRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(60028..60738)
                     /locus_tag="ACHLAG_000349"
     CDS             complement(60028..60738)
                     /locus_tag="ACHLAG_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0653658.1"
                     /translation="MELVNTLFASLAGTDPFTDVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(60754..62262)
                     /locus_tag="ACHLAG_000350"
     CDS             complement(60754..62262)
                     /locus_tag="ACHLAG_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP0653659.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFRQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNVDNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNDDKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT        15000 a        17060 c        15643 g        14660 t
ORIGIN      
        1 gggaccgttc agtacggaat ttagcttgac ggtgctatcc agcattaaat actggcaata
       61 ctctcacgca aaatccgcag caccgtctct tcaccgctgc gattcgggtt aatgcgaatc
      121 gcacaatgtt ctgattgtgg attcgcctgg cgaaacgttc cggaaaggcg ataaaagagc
      181 ggcgggattt catatttcga ctctgcaccc accgggtaag gcaaggcacc gcgcttttgc
      241 gcctcttcca gcactctggc ggcaatcggc tgatgaaact cgacaatcaa caccttcgac
      301 tgcgcattag caatcaccgc gcttttcact tccggcaccg caccaccgtt aagcaaagcc
      361 agcaaccgtt cagatacccc tgcctgcacc gcgtgcatca ctggcgcaaa caccagacca
      421 cgcaatactt ccagcgcctg tgcgccctgg atctggctac cgccggagta aagcgtggcg
      481 cgaatacggt tgataacatc agcatcgccg accactgcac caacaccctc tggcccaaat
      541 agcttgaagc aggaaaatgt cgagacattc gcgccgcatt cacagccgat tcgcgccacc
      601 ttcatcaccg catagttgtc atcggttaac gctggaacac ctgccgcgcg caacgttgcc
      661 agcacatctg ccagcacgta gctgtcctgc ggctgctggc gcgtatgctg caccagcgcc
      721 gcatccggtt gttgctcgtc gacgacctgc ttcagtgccg acaggtcatt gaaatcaaca
      781 gtaataagcg tcagccccat ctgctcaata ataacccgtg tcgtcgggta aacaggcgcg
      841 tcatgcacca gaagacgctg ccccggtttg agcaaagccg ccagcccggc gcgaatcgcg
      901 ccagtccccg cgccctgcac cagcgccgca gcctgtgcgt gaaatgcatc agccagcacc
      961 tgttccacac gctgggtaac gcgcggttga ttcagtcctg gcgttaagcc taaatcaccg
     1021 ccagtaagaa actcgctgcc ggggaaatgg cgacaaatgc tatccaccag cgcaaactgc
     1081 ttttgctgcg cctcaataat cgtcaggctt tgcagaggaa acgtcttcat aggtgccttc
     1141 cttaagccgg aacgaacagg ccaagccagt aaagaacatt cagcagaata ccggtgatca
     1201 ttactgccac gaccggtgcc gccattttct gtaccggacg gcccagggat tcgttgagga
     1261 agtaaatggc aaccgcgata gagaatccgg tataacccgc catcttaatt gctgcgaaaa
     1321 tcgaaccgac cagcagcgcc acttccatca gcatattcat ggcgttacgg atgttatccg
     1381 acgcattacg caccgacgga tagcgcccca gccatttgcc gatcgaacga agcaataaaa
     1441 cttctgccga aataaccact gcacctaata ccgctgcaac catcggattc ggcgagagat
     1501 agcccaccgc ataaacaaag gtaaagcccg caactgcata cacacccgtt gctaacgcgg
     1561 tggtggcaat caacggtaca aaacccagcc cgcgcataaa ttctgccagt gccgcctggt
     1621 tgatcagcgt ttgcgattgt tccggcgtta cgcctgcgga gtacgctttc tccagagtga
     1681 agatcgacac ttcactgccg gcgaaaatct tcatgctggc aacggcggca atcaatgctc
     1741 ccacgatggc gatatagggt aagtttttga taatccgcga cgtgcgttct tcaaacaccg
     1801 aaagcccgct ggcatcaata tcattttcat cacgatgacg caggtcgtga gttatcgcga
     1861 tccccagcag catcaccatg ccaataaaga tttcgatgga ttcagggtta agatgtggga
     1921 aatagcgcac gactaccaca cgggtcatca gaacaaccac ggcggcgacc agactttgct
     1981 tccagccaaa ctggtagaaa atcgccacca gcgggaacag tgcaaaagca gaaaccaccg
     2041 gcgagcttaa ttccccgagg ctacctaata catccaccgg cagcgcggtc agcagctggt
     2101 ttactggcaa caggcaagta aggatcaaca cgccccagat agcgccaaga ccaaacgcca
     2161 tcaggctgtt tatcgccagt acgccgagga tatcggtagg aagaaagagt aaccatgcgt
     2221 tgagcagccc ggttttcagg gtaaaagaga tccccaccga ggccacaaaa ccaatgctca
     2281 gaccaaaagc gatactcccc gcctcgcgac gattcatata gccttcaatc agttgcggca
     2341 ggatcgggcg gatgccgtca tgaaaaacag ccgccgagcg atgcgccaga agcgatgtca
     2401 tacccgtcag gcacgccacc acgataatct gaatatacag atccatagtc gtgcctttta
     2461 tttcagatgg ttaatcagca tcggaatcgc gtgttcgacg tgctcaacgg aaaggccaaa
     2521 cgccactttc ccttcagcga tcattttggc gatatgctcg tctttcgctt taatgcctgg
     2581 tttggcaatg gtgcaacttt tgttatagcc aatcaccgcg atggcaatcg acaatgccgc
     2641 gcccgcaccg gtattacacg caccgatgta gtaatccagt tgcccggatt tcactttcat
     2701 tgccgcttcc atgtcgttgt gaatgaaaac ttcaaaactg ccaggagccg tcgcttcaat
     2761 ggttttttta atctgctcac gctgtaagcc tgcaacgcca atctttttca tgagtctgtc
     2821 cttattggaa aaattgagag ggattttcac gtaacatcac atccacatcg gcctgactga
     2881 atcctgactg gcgcaattgc ggaataaagg tggtcaataa atagtcatag ccataaccac
     2941 cattggcttt taaatgggag cggcgcgtaa tatccatcga cagcatgacg cggttcagca
     3001 acccacggtc acgtagcgcg tggagcatcg caatgcgctt ttcgtccggg tagtaactgt
     3061 tcttaccaat ggtgtcgaac tgcacgtacg cgccgagatc gatcatcttc aaaatgttgt
     3121 cgagattgtc tttcagatcg cagtgaccaa cggtgacgcg cgaaagatca actccgtggg
     3181 cttgcaacag agccagttgt tccagcccca tggtgctgaa cgaagtatgc gtggagatcg
     3241 ggcgtccggt ctggttatgc gccagcgcag cggcaataaa caccttctct tccagcggcg
     3301 taatcttccc ttcgctagag ccgatctccg cgataatccc ggctttcagg tcggtgccat
     3361 cgataccctg ttcaatttca tcgaccatct cctgcgccag ttcctgcacg ctgcgggtcg
     3421 ccacatgttc cgggaaaaac gcgtcctggt aataaccggt acaggccacc acgttgatcc
     3481 ccgtctcgcg cattacatca agcataaatt gcgcattgcg ccccatgtaa cggttggtca
     3541 tctcaatcac attacgcacg ccccgggtca tcaggtcgtt catctcctgg caaatgaacg
     3601 catactgatc aaggcggcag tccacgttgt ttttaaagcc ggagagatca atatgcagat
     3661 gctcatgggc gagggtgtaa cccgtcggat caaaactcat agggtgtctc ctgcaaaccg
     3721 gagggaggaa agaaaagagc gaccattttg tggcggtggc gcgcggaaaa attcacacac
     3781 ggtagccccc acatcagaaa gcgtggtgcg cacaccgagc tgcgtagcga tcatcccttg
     3841 ctgataaacc agcactggca ccacttcgtg ggtatggtgg ctgtgaccaa tggtcggatc
     3901 gttaccgtga tccgccatca cgaccaggca atcatctggc tgcatcgcct caacaagccg
     3961 ggcaaggtta cggtcaacga cctgcaaacg ttcggcataa cgtgcgacgt cttctgcatg
     4021 accagcgagg tcggtttcct ggatattgat gcaaataaac gccgtcggat gcgtgttaaa
     4081 ttcgtcgagg gtgatatcca taatccgctg gctatccacc agattttgcc agctcacgcc
     4141 ataaggattg ttgacgatat ctgccacctt acccaccagc acggttggca cgcctgcttc
     4201 atacagtttt tgtggcacct gcactttttc atcgacgcca tagcccatat gcacgacctg
     4261 gaaaccgttg tcataagcgc cagaacgcgg cgcattgata ccaataaagc gcccttcttt
     4321 gctttctgcg gcatcgagaa tgcgttgact gtcggttaac aggccaccaa atgtaatgac
     4381 ccgaccgacc tgtacctgct cacgcacgat acgaccaatt ttgattgcgt cgtcaaaaga
     4441 gatcacagag agattggcgg taatgttata gacctggcct aaatccgcct cgagattatc
     4501 gccaatcgca accgcctgat tgacccacag aaattgcagt tcatcgccac ggcgctccac
     4561 ctgccaaccg gcggatacta atgcctgctc aacacggtca atcacatcgc aaaaaggcat
     4621 ccgcagcggc ggtaacgggc gcgtgcctaa aatttcctga tgccccataa aggtatcgcc
     4681 accttcatgt tgcagctctg ccacgcccca ggttgcggaa tctgacggct gcatatcgcc
     4741 tggcgcataa cccaatgcgt tgattagccc cagcttctcc agcgttggta gctgcaaatg
     4801 cggcaactgg ctcaggatgt gaccacaggt attcgctccc gcatcttgcg gacgcaccag
     4861 cgtgacatct ttcattgccc ctacgccaaa gctatcaatc actaacacca caaatcgcgc
     4921 cattacgccc ccagagagtt tccaagactg tcataacgac caacgatttt cggttcgcca
     4981 cggtgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcggaaa
     5041 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
     5101 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
     5161 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
     5221 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
     5281 ccctgctggt ttgccggaat agtgcccgtc agcgcatgac cgggttcggc atgagtcaca
     5341 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggcgc gttcagttgc
     5401 tcaagtgcaa taccagattt cgccagttga tcccgtgcct gtatcagcgt gtgaagattc
     5461 ggtgtcggca aaacttttcc ggcagcctca tcccaaagca ggcaagggaa atgggtaagt
     5521 ccggctaaat gcagccccgg cagtttctta atttcagcaa ccacctcgtg cagcgaatgc
     5581 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
     5641 agcacaaact gcacacgtcc ggccttcacc gctgccgcag aaacttcccg cgctttgtcg
     5701 agagtaaaca cggtgatgac gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
     5761 gggatttgta ccagatgccc ctgatgcgcc accgacaaac cagcgcggcg catgactcgc
     5821 gcctctttgt aatccaccgc cacaatgccg ctgtaaccta atgccagcaa tttttccgcc
     5881 agccatgggt tacggccaaa ctgcttggtc atcagataca gttcaatgcc gtaaagccgc
     5941 gccgtctcaa tcagtcgctt accgttttcc agtacctgat ccacgtcgat cacccagctg
     6001 tccggggcga tcttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
     6061 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
     6121 ttcatttttt tgaatattta ggttgtgcgg cgtggatttg tcgggagctt caaacctctt
     6181 cgttagcagc catccataat ccataaaggt tggccagcaa atagccttct tcacacgggt
     6241 taacttccag tgcgaattcc ttcaacaaca cctgatgcaa ttgcacaacg gcttgccagt
     6301 ggctggattg cgccagctct gccagcaatt cgttatccag cggctctatt tcttcaccac
     6361 ggcgactgcg catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc
     6421 gcaccggcag atggcactct gtttccagta cgttgacgac ctgcatcatg cctttgcaga
     6481 tgtccttatc aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg
     6541 cctttcgttt aataactcgg ttcctgttcc ccactcacta cccgttgttg atgggcgagc
     6601 agggcgtgtt tatctacaac ggcacgcagc agttgttgca ttgggtaatc atcgactcgc
     6661 gtcaggacca cggcttcggt ggcctgtaaa aatcgcggat cgtctgtgag cggcgtcgcc
     6721 tctaatccca gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca
     6781 tcgcctttaa caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca
     6841 ctaccgccaa aaaaaacatc ggtcatgatt ttctgatccg ccgaacggtt atccagcccc
     6901 acgcgcttca cgtttgcgga ctcgccttta cggcaaatca actggtgctc gccaacgtag
     6961 gtgtgcggcc ccaactccag cgcgaggcat aacccttttt gcgtgagata actttccgcc
     7021 gccagtcggg aaaccaccgc catgtcatac acgccgttaa gtaaacactc cacgcgaata
     7081 tccgcgccgc gcatatgcgc atagtaaaaa ggaatgccat caaactgagc tttcagcccg
     7141 ctcgctaaac cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg
     7201 atatccacat gactcagcaa tgctttgtta tccatctcga ccagataact gccattgcgc
     7261 ccacggcgtt caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc
     7321 aggccaaccg atgaacggca ttcagttgcc agctcatcta tggttttcaa cctattaccg
     7381 catttttcac ccaacaaata acgggccagc gtcgtgataa cgacgccttc ttttttgata
     7441 aacgttcgac gcataatata ttttcaataa aatgaatgtt tatatcttca ttaaaatgaa
     7501 gataagtggc aactgtgaga tgtggaaacg gcgaggcgct tcacattttt tcggattatt
     7561 gcaaatgagg ataaaaaaac cgggtttccc cggtttcaga gtgatgacga agtgatttat
     7621 tcgacaatgc gagcaaaggg taattcccac tggcgcttgc ctgctcccct caccctaacc
     7681 ctctccccaa aggggcgagg ggaccgtccg agcgttttga tattacggct tcgccacaaa
     7741 accaatcgct tcgtacaccg ctttcagcgt acgggaagcg tgtacactgg ctttttctgc
     7801 gccgtctttc atcacctgtt gcaggaaggc ttcatcgttg cggaaacggt gatagcgttc
     7861 ctgcaattca gtcagcatac cggaaacggc atcagccact tcacctttca gatgaccata
     7921 catcttgcct tcgaactgtt tttccagttc tgggatgctc tggcccgtta ccgcagaaag
     7981 gatatccagc aggttggaaa cgcccgcttt gttctgcaca tcgtagcgaa ctaccggcgg
     8041 ctcgtcggag tcagtgaccg cgcgtttgat tttcttcact accgatttcg gatcttccag
     8101 caggccgata acgttattgc ggttatcgtc agacttggac atcttcttgg tcggctccag
     8161 cagcgacatt acgcgcgcgc cagatttcgg aataaacggc tccggcacct taaagatctc
     8221 gccatacagc gcgttgaaac gctgggcgat atcgcgactc agttccaggt gctgtttctg
     8281 gtcttcaccc accggtacca gattagtttg atacagcagg atgtccgcag ccatcagcac
     8341 cggatagtca aacagaccag cgttgatgtt ctcggcataa cgcgcagatt tatctttaaa
     8401 ctgggtcatg cggctcagtt cgccgaagta ggtatagcag ttcagtgccc agcctaactg
     8461 cgcatgttct ggtacgtggg actgaacaaa aatggtgctt ttctcaggat cgataccaca
     8521 agccagatac aaggccagcg tatccagcgt cgctttacgc agcttctgtg catcctggcg
     8581 cacggtgatc gcgtgttggt caacgataca gtaaatgcaa tggtagtcat cctgcatgtt
     8641 tacccactga cgcagcgcac ccatgtagtt accaatggtc aattcacctg agggctgtgc
     8701 gccactaaaa acgatgggct tagtcatttt tcgattcctg attttcgcta tgcggaagcc
     8761 ctaatgcggg cagaaggtca tttatagact gataaattac atcaggctgg ctgagatcga
     8821 tagcctcgcc gtagttatat ccgtaggtta agccaacgga tgggcaacct gccgcttttg
     8881 ccgcctgaat atcattgcgt gagtcgccga caaacagcat ctgttgtggg gcaattccca
     8941 tccgctcagc caccagtaac agcgggtccg gatgaggttt tttgttttgc acatcatcgc
     9001 cgccgataac cacgctaaag tatttggcaa tatctaaggc ttcgagcagc ggcgcgacga
     9061 acggcgtcgg tttgttggtg accaggccta gcggcaggcc tttagcctgc aacgcgccca
     9121 acgtatcggc aacgtgcggg aacaaaaacg tcccctcttc ggcaacctcg ccatagtagc
     9181 gatcgaacag tttacgcaga atacgtacct gttcttctgc cggaatgtcg tcatcaacgg
     9241 gcggtttacc cattgtttta cgctgagtcg cacgttcctg acgcgcccag gtcaatgcgc
     9301 gctccatcag aacatctgcg ccgttaccaa tccaggtaat aacgcgttct tcacctgcga
     9361 cgggcaactc cagcgcatac agcgccatat ctaccgcagc agcaagaccg ggagcactgt
     9421 cgaccagcgt accatcaaga tcaaaagcga cgccgcgaat atcttcaaac ttattcatga
     9481 cttacctttg ccagttcact gcgcatttca tcaatgactt ttttgtagtc tggctggtcg
     9541 aagattgccg aaccggcgac gaacatatcc gcgcccgccg cagcgatttc gccaatgttg
     9601 ttcaccttca cgccaccgtc tacttccaga cgaatgtcaa agccagactc gtcgatacga
     9661 cgacgtactt cgcgcagttt atccagtgtt tgaggaatga acgactgacc gccgaaacca
     9721 ggattgacgg acatcagcag gatcacatcc agcttatcca tcacgtaatc cagatagctc
     9781 agcggtgtcg ccgggttaaa taccagtccc gctttacagc cattttcttt aatcagttgc
     9841 agcgtgcggt caacatgctc ggaggcttct ggatgaaagg taatgatgct ggcaccagcg
     9901 gcagcgaaat caggcacaat gcgatcgaca ggtttcacca tcaggtgtac gtcgataggg
     9961 gcggtaatgc cgtagttacg caaggatttc agcaccattg gcccaatcgt cagattggga
    10021 acatagtggt tatccatgac gtcaaaatgc acgacatcag cgccagctgc cagggctttt
    10081 gcggtatctt cacccaggcg ggcaaaatca gccgacagaa ttgagggggc aatcaaatac
    10141 tgtttcatcc gcttctcctt gagaattatt ttttcgcggg tgaaacgact cctggtttgt
    10201 acaaagccag cagttcgtcc acctttttac gtgtgccgcc gttgctgctt atactgcgtc
    10261 gaactttgac gacatgcaat tttgcgcgct gataccactc acgcgttaac atcgtatcgt
    10321 gattggagat cagcactgga atatggcgct caaccagacc ttcggcgatc tccgccagat
    10381 gcgcttgttg ttcaagcgta aaactgtttg tgtgatacgc cgtaaagttg gcggtcgcag
    10441 acagcggtgc ataaggcgga tcgcaataga cgacggatgc atcatctgcg cgcgccatgc
    10501 tatcggcgta agactcacaa tagaaaaagg cattctgcgc tttttcagcg aagtgataca
    10561 actctgcttc cgggaaatag ggttttttgt agcggccaaa cggcacgtta aactcaccgc
    10621 gcagattgta acgacacagg ccgttgtaac cgtagcggtt caaatataaa aacagtaccg
    10681 cccgacggaa cggatcctgg cttttgttga actcttcgcg gaactgatag tagacctcgg
    10741 cgcaatttgt ttcgggaaca aacagctcgc gtgcggcctg tacgtactca tcagtacgca
    10801 tcttcacaat gttatagaga ctgatcaggt cgctattgat atcggcaagg atataacgag
    10861 aaaagtcggt gttgagaaac accgacccgg cacctacaaa aggctcaacc agacattcgc
    10921 ccttgggcaa atgccgttta atatcatcaa gcaggggata cttgccccct gcccacttca
    10981 aaaaagcgcg atttttcttc atgctgacta actaattaca ccttctccgg ctgtggagaa
    11041 agctccgaca gcatccagcg cttcaggcaa ctcccgcaac gataacccat tgcgggagat
    11101 aaccttgatt acttcagatc ggcctgtacc tgacgcagcg gtttcgccca cgggtttttg
    11161 gcctgaacat ctgctggcaa tgtagatacc gcttttttcg cctcttcttt cgaagcgtac
    11221 acgccagaaa ccaggacata ccacggctga ccattacgcg tcgtttcata gacaacgtag
    11281 tttttcagat tctctttctt cgcccaaccg ttcaggttgt cgtagttaga ggaactgctc
    11341 agctgcagag tgtaatggct ggacggtgcc gatttcaacg aaccaacatt acctgcggtc
    11401 ttccctccag cggctggtgt tgccgtggtt tgcgccgggg atgccgtctg tactggagcc
    11461 gtagtcgccg tctcttttgg tgctggcgca gaagtcgcag ccggtgcctt cgtgctcgcc
    11521 acaggagcag ctggttcagt acgcttcggc gtctgtgcta ccggcttcgg ttccgttttc
    11581 acggttgctt gcggtttttt cggttcaatc accgcctgct gacgagctgg acgcgtagtg
    11641 gccggacgtt cagcagtttg cgttttcgcc gtgtcacgcg atgcattgcc attgcgtact
    11701 ggcgcgacag ttgctggttc agtcggcaat gtagaattga ccgccacatt gttcaactgt
    11761 tgctgatttt gtggctgggt cagcgcattg ttcaggtcac cctgcacttc aacacgttgt
    11821 tgaccatccg ttgccgccgg ggtttgccct tgagtcggcg tagaagagat cggtggcaga
    11881 gaaacatcct gctgagtatt ttccgcagac gtggttcccg gcgctggctg caccccattc
    11941 gcctgatcgg tcgcattgcc tgcaagatca atactcttct cgccagacgc ggtttgatcg
    12001 ctggaagagg tcgagggggc ttttagcgca gaaccgatac cgatgatcaa cagcagtaga
    12061 accagaatgc cgacgcccat catcatatac tgacgagaag cgggtttact ggctgctttt
    12121 ttgcgcttac gcggacgacg ctctacgcgc tcttcatcca cggtttcatc ttcggattcg
    12181 tcaatttctt cttcgatttc cggttcctca ttgcgctctt tttgcgcacg agtcggacgg
    12241 cgatcgtcag tgtcatcaag ttcaatatca tcaaaattga tctgcggttc gccacgttca
    12301 gtacgctcag aacgttcaga agattgacga gaacgaccag tacgacgatc gctgggatcg
    12361 ggtttcagct cgtcttctgg tttgaattca tccatttaac accccactaa aaggttaatg
    12421 cttaccacgt tgcaattaac ctgaagctaa tagaccgctt gataagcggc ctgacctttc
    12481 ttgttgttac gctgattgac aatcggcaat ggcgttaaga acaagctcgt gcgaaacgcc
    12541 gctgcgaact tcactcttac caattgccaa cggaagaatt aagcgtatct ctcccgcaag
    12601 gactttcttg tcacgcagca tatgcggtaa atacgcctgc gcggacattt cgcgcggccc
    12661 attgaccggt aacccagccc gcttgagcag ggttataata cgctgcgttt cggcagaact
    12721 aaactgcccg agacgttccg acgtccgcgc cgccatcacc atacccgcag cgaccgcttc
    12781 accatgtaac caattgccat accccatttc agcttcaatg gcatgaccaa aggtgtgtcc
    12841 cagattcagt aaagcacgta acccggtttc gcgctcgtcg gcggcgacaa cttctgcctt
    12901 cagttcacaa caacggcgaa tacagtacgc cattgccgga ccgtccagac gcaacaacgc
    12961 atccagattc tcttccagcc agttaaaaaa cgcaccgtca agaataatgc cgtatttgat
    13021 gacttctgcc agccccgacg ctaactcacg cgggggaagc gttttcagac agtcgagatc
    13081 caccaccact gaagcaggct ggtagaacgc gccaatcatg tttttaccga ggggatggtt
    13141 gaccgcagtt ttgccgccaa cggaggaatc gacctgcgac agtaacgtcg tcgggacttg
    13201 aatgaaacga acaccgcgct gataactcgc cgccgcgaag ccggtcagat cgcccactac
    13261 gccgccgcca agcgccacca gcgtagtatc gcgaccatgc ggcttttgta acaacgccgt
    13321 aaagacggta tcgagtacag ccaggctttt atactgctcg ccgtcaggga ggataacgct
    13381 atcgacgtta acacccgcct gttcaagtac gccgcggacc ttatcgagat acagaggagc
    13441 cagggtttcg ttggtgacca acatgacctg ctcgcccgat ttcagcggta agaatgaagc
    13501 tggttcatta aacaaaccag atgcgatggt aattgggtaa ctacgttccc cgagagtaac
    13561 gacaatcctc tccataacgc gacatccacc ttaattactg tacccgcaga cgagtgtata
    13621 taaagccaga attagttgct ttccagcatg tgaataatct ggtttgcaac cactttagcg
    13681 ctttgatcat cagtacgaat ggtcacgtcg gcaatctctt catacagcgg attgcgttca
    13741 ttggccaacg cttccagaac ttcacgcggc ggtgtttcaa cgtgcagcaa cgggcgtttt
    13801 ttatcacgct gcgtgcgtgc aagttgcttt tcgatggtcg tttcaagata aacgacaacg
    13861 ccacgagcgg aaagacggtt acgcgtttca cgggatttca cagagccgcc gccagtagcc
    13921 agcacaatac cctgtttctc ggtcaactca ttgatgacct tttcttcgcg atcgcggaag
    13981 ccttcttcgc cttctaaatc gaaaacccag cccacatcag ctccggttcg tttctcaatc
    14041 tcttgatcgg aatcgtaaaa ttccatattg agttgttgag ctaactggcg cccaatagtg
    14101 ctttttccgg cacccatagg cccaaccaga aagatattgc gtttctctgc cattttttcg
    14161 gtactactaa gactattcgt taatgataaa cccgcttcgc tcagagagcg ccgcaggaca
    14221 tgaactgaaa cctcataaga tattgcgaga gtcagactga aaattatctc aatactccag
    14281 cgggtttggc aactgaataa atcacccatc cctctgcata tctggtcgct gcaagcgcgc
    14341 tgccttgcta ccaccgctct ggcgataaat caccgggtaa gattagcgta aaaaagacag
    14401 caaaatgccg cctgaatgat aaatcatcat catggaacgc aggcagcgaa gagcaccaac
    14461 tctcaaatcg gtactccttg tatgctaaat acctgcgcgc gtcaaataga tgaaacacgt
    14521 tcagcataaa aacattaccg cttttacggc tgtttactca ctggaaacca gtcgtggcgt
    14581 gataaacacc actaactcgc gtcgttcatc ttcttttccg tcatgacgaa ataattgccc
    14641 gaaccaggga atgtcgccaa gcaacggtac gctatcctga cccgatttat ttttacgggt
    14701 aaaaatgccg cccagcgcca acgtttctcc gcttttgacc tcgacctgcg tttcgatctc
    14761 ctgcttatca atcgccagca cttcgccatc ggcctgctgt agcacctgcc ccggaacgtt
    14821 ctggctgatg tgtaatttca gccggatgcg acctttttgt aacaccgtgg gcgtgacctc
    14881 catccccagg acggcctctt taaattccac cgacgtcgcg ccactttccc cgctggaaac
    14941 ctgatatgga atttcgctcc cctgtttaat gctggcaggc tgaagatgtg aggccagcag
    15001 acgcggactg gcgataatat ccagctgctg tttttgttcg agcgcggaaa gctcaagatc
    15061 cagcaagcgt ccgttgatgc gcccaatgtt aaaaccgaca tgcgttgtcg ccgtcgctac
    15121 ggagaggtcg ctaccaagcg tggtgacttg cccaacgcca ccagcgtgtt gcgcatcggc
    15181 cagcgtccat ttcacgccta actcacgcaa acttttttca ttaatggtga caatatgcgc
    15241 cgacagctca acctgcccga ccggcagatc catttgcgct acccactgtt caagcgcgct
    15301 taacgccgtt ttgttatctc gtagcaaaag gcgattggtg cgtttatcga cggtcatact
    15361 ccctttggca ctcagtagct tctcccccgc tttcgccagt tctcccgcgt cggcgtattg
    15421 cagggttata ctgcgatttt ccagcggcag atttgcctgc gcccgcgcct gctccgcctc
    15481 ctggcgggcg atattgttat tctgccaggc aatggaatgc actgagagaa tgttgtcttc
    15541 ctgccgcgtt atcagtccgg cgcttttcac tacagtttgt agtgcctgct tccagggaac
    15601 atctgttaga tgtaacgaca ccgtaccgct gacgtctggc gacacgacca ggttcaactt
    15661 ctcctgttca gccagcgcct gcaacacctg agctaccgga acgtcatcca ccatcagcgt
    15721 cactttttgc ggctttgccg cctgtacgcc gggtatcagc atcaacagta gtgcggctat
    15781 ccattgcttc atttgtatct ccttgccgtt gccacaacca ttgtggcggt tcgcagtttg
    15841 tcccggtacc cagcgttagt acgtctggcg tcagctgtaa aattgtccag ccgttttcca
    15901 gcacatcgtt ttgctgcacc cgtcgccatt tcttttgccc gtcttttatt acaccgatga
    15961 tgcgctcgcc tcgccctacc atcccctgat agcgccactg gctaagttcg ctaatccggc
    16021 atagatcttc cggcggttta aaagggtcac gcataccggt taaaaggcac aatgcaatac
    16081 ctgccaacaa ccagcgttta accctcatgc ggcgtctcca gttgtagcgt gaacaaaaga
    16141 tcatcacctt ccacgcttaa cgaaaaacgg ctcacgctga cgttgcgctc tgccagccgt
    16201 gtaaatgccg acggcactgc ttcccacagc gttttcaacg ccaactcgcc tccctgcgcg
    16261 gatggatgcc aggaaaccag ttgcgcgccg gataactgaa aatccagtgg cgaaaagggc
    16321 agcgtttttt cctcgctgaa gggagtggtg tctaccaggc gatacagtgc ggcccactgc
    16381 tggtgatgac ttgcccgtag tcgaattaat gcgtcgcgct cttcatggtg tgtcgatgat
    16441 agaaaaatga gcgtaactaa catcagcaac cagactgccc agcaaagctg gcggaggcgg
    16501 ggtgatgtgg cgaaccacca gtcaaagaac atgttcatcg ctaaccttcc ttgttaactg
    16561 atactcaaat tgccagcgtc cctgcgcatc ctgctgcgtg gctccccgct gattgagatg
    16621 aaaagacgca tcctggcgga gtgacgtttc tagtgcgttt aacgcggtaa tgcttgttgt
    16681 cagccccttg atctccagcg ttccctgctg ccagcttatc gttgtcagcc aggcgtgctc
    16741 gggtaaaagc gccgccagtg cttccagcgc agattgccag tcgcgggtaa attgtcgctg
    16801 gcgctgccgt tgcgaacgct gttcgcgtaa ctgctgttgc tccagcaaac gtggcttcgt
    16861 tatctgtaag ctgcgggcga gttgttgttc cgcctgaagc aaaactgcgt ctatgcgcgc
    16921 ttcggcgctg cctgtcagac gcagtattag cgttatcccg acggccagca gcagaggcgc
    16981 aacgaacatc agcaaccaga aacgcagaaa agcggtccgg cgttgctgtc gccagggcaa
    17041 aaaattaatt ggcgggttca tcagtacgcc ttcccaagcg ccagccccag cgcgatggca
    17101 aagtctccac ctgacggcgg tagcggcggc tgacgaacag aaacggcctc ccagggatca
    17161 aatccccctt cgccacatat cgcgacgctt tcgggatcaa cggataacgc tgccgccagc
    17221 tccttcgcgc tagtcatccc caccgccagt ttgcgccccc agcgatagcg tgtcgcccac
    17281 agccactgtt cgttatcacg ccaggccaga cattgctgat gagaaggtaa aaaaggcagg
    17341 aatcgctgta atgcactggc atccggggtg atcgcactaa catgaacacg caacctttct
    17401 gccagcgtaa gcagcgttgc cagctctttg ctttgcgcgg cagtcacgtt ataagcgggg
    17461 ctgagtgagt cttcgctata atcgaagcgc agggagtccg gatccatatc cagctcgcgg
    17521 gccatcgtcc ctgacagcca ggccgtttgc tcccgctcac caagggacat cgacgggcgc
    17581 ggaaatgacc gctgtaatgt gcgactggcg ggaaacgcca acataatgtg atgacgctgc
    17641 ggcagttcgc gactccaagg taacaacgtt ttagccagct gctgcgcatc aacaatccgc
    17701 ccatctttga taatgtcgtt ctccagcggc aaccgccacc agcgttgcaa aaagcattct
    17761 tttgcgcccc gtacgatcgc aaccgctacc gcttcttgct gttgtaaatg caaaccaatt
    17821 tgccagatct taaatgccat tgtgatgatc tccttatcac ccgtcactct gacgggtata
    17881 tcaatgcgtc tggcttgcct ttatactacc gcgcgtttgt ttataaactg cccaaatgaa
    17941 actaaatggg aaatttccag tgaagttcgt aaagtatttt ttgatccttg cagtctgttg
    18001 cattctgctg ggagcaggct cgatttatgg cctataccgc tacatcgagc cacaactgcc
    18061 ggatgtggcg acattaaaag atgttcgcct gcaaattccg atgcagattt acagcgccga
    18121 tggcgagctg attgctcaat acggtgagaa acgtcgtatt ccggttacgt tggatcaaat
    18181 cccaccggag atggtgaaag cctttatcgc gacggaagac agccgcttct acgagcatca
    18241 cggcgttgac ccggtgggga tcttccgtgc ggcaagtgtg gcgctgttct ccggccacgc
    18301 gtcacaaggg gcgagtacca ttacccagca gctggcgaga aatttcttcc tcagtccaga
    18361 acgcacgctg atgcgtaaga ttaaggaagt cttcctcgcg attcgcattg aacagttgct
    18421 gacgaaagac gagatcctcg agctttatct gaacaagatt tacctcggtt accgcgccta
    18481 tggtgtcggt gctgcggcac aagtctattt cggtaaaacg gtcgaccaac tgacgctgaa
    18541 cgaaatggct gtgatcgccg ggctgccgaa agcgccttcc acctttaatc cgctctactc
    18601 aatggatcgt gccgtcgcgc ggcgtaacgt cgtactgtcg cggatgctgg atgaagggta
    18661 tatcacccaa caacagttcg atcagacacg cactgaggcg attaacgcta actatcacgc
    18721 gccggagatt gctttctctg cgccgtacct gagcgaaatg gtgcgccagg agatgtataa
    18781 ccgttatggc gaaagtgcct atgaagacgg ttatcgcatt tacaccacca tcacccgcaa
    18841 agtgcagcag gccgcacagc aggcggtacg taataacgtg ctggactacg acatgcgcca
    18901 cggctatcgc ggcccggcaa atgtgctgtg gaaagtgggc gagtcggcgt gggataacaa
    18961 caagattacc gatacgctga aggcgctgcc aacctatggt ccgctgctgc ctgccgcagt
    19021 caccagcgcc aatcctcagc aagcgacggc gatgctggcg gacgggtcga ccgtcgcatt
    19081 gagtatggaa ggcgttcgct gggcgcgtcc ttaccgttcg gatactcagc aaggaccgac
    19141 gccgcgtaaa gtgaccgatg ttctgcaaac gggtcagcaa atctgggttc gtcaggttgg
    19201 cgatgcatgg tggctggcac aagtgccgga agtgaactcg gcgctggtgt cgatcaatcc
    19261 gcaaaacggt gccgttatgg cgctggtcgg tggctttgat ttcaatcaga gcaagtttaa
    19321 ccgcgccacc caggcactgc gtcaggtggg ttccaacatc aaaccgttcc tctacaccgc
    19381 ggcgatggat aaaggtctga cgctggcaag tatgttgaac gatgtgccaa tttctcgctg
    19441 ggatgcaggt gccggttctg actggcagcc gaagaactca ccaccgcagt atgctggtcc
    19501 aattcgctta cgtcaggggc tgggtcagtc gaaaaacgtg gtgatggtac gcgcaatgcg
    19561 ggcgatgggc gtcgactacg ctgcagaata tctgcaacgc ttcggcttcc cggcgcaaaa
    19621 cattgtccac accgaatcgc tggcgctggg ttcagcgtcc ttcaccccaa tgcaggtggc
    19681 gcgcggctac gcggtcatgg cgaacggtgg cttcctggtg gacccgtggt ttatcagcaa
    19741 aattgaaaac gatcagggcg gcgtgatttt cgaagcgaaa ccgaaagtag cctgcccgga
    19801 atgcgatatt ccggtgattt acggtgatac gcagaaatcg aacgtgctgg aaaataacga
    19861 tgttgaagat gtcgctatct cccgcgagca gcagaatgtt tctgtaccaa tgccgcagct
    19921 ggagcaggca aatcaggcgt tagtggcgaa gactggcgcg caggagtacg caccgcacgt
    19981 catcaacact ccgctggcat tcctgattaa gagtgctttg aacaccaata tctttggtga
    20041 accaggctgg cagggtaccg gctggcgtgc aggtcgtgat ttgcagcgtc gcgatatcgg
    20101 cgggaaaacc ggtaccacta acagttcgaa agatgcgtgg ttctcgggtt acggtccggg
    20161 cgttgtgacc tcggtctgga ttggctttga tgatcaccgt cgtaatctcg gtcatacaac
    20221 ggcttccgga gcgattaaag atcagatctc aggttacgaa ggcggtgcca agagtgccca
    20281 gcctgcatgg gacgcttata tgaaagccgt tcttgaaggt gtgccggagc agccgctgac
    20341 gccgcctccg ggtattgtga cggtgaatat cgatcgcagc accgggcagt tagctaatgg
    20401 tggcaacagc cgcgaagagt atttcatcga aggtacgcag ccgacacaac aggcggtgca
    20461 cgaggtggga acgaccatta tcgataatgg tgaggcacag gaattgttct gattaaaaag
    20521 gcgcttcggc gccttttcag ttggctgaca aagtgcattt gtttatgccg gatgcgggtg
    20581 aacgcgttat ccggctaaca aaatcgtgaa aaatcaatat attgcaggaa acccataagc
    20641 ctgataagcg ttgtgcatca ggcaaacttc acgcatttac actcgcccct gccctttcaa
    20701 ccattcgcgc acgaggaaca gcgcgctgac attacgcgct tcgttgaagt cagggtcttc
    20761 cagcaaatcc atcatatgcg ccagcggcca gcgcacctgt ggtagcggct ctggctcatc
    20821 gccttccagc gattccgggt agagatcttg cgcgaccacg atattcattt tgctggaaaa
    20881 gtaagacggt gccatgctga gcttcttcaa aaaagtcaga tcgttcgcac caaatccaac
    20941 ctcttctttt agctcgcggt tagcggcttc gtagacgctt tcacccggat caattaatcc
    21001 tttcgaaaaa cctaattcgt aggattcagt tcccactgcg tattcgcgga tcaggatcag
    21061 gtgatcgtcc acaatcggca caatcatcac tgcttcccgg ttggttggac gcattcgttc
    21121 ataaacacgc cgcacgccat tgctgaactc cagatccacg ctctcgacgg taaacagtcg
    21181 ggaacgggct acagtttcaa cattcagaat ggtgggtttt tgtaatgatt tgctcatcgt
    21241 gggatctatg ctgtgaaatc agcggttatt gtgcgatatc ggacacgctt tcggcaatgt
    21301 gaattgcatg ttatttacat ttatgtaact taataaataa tcgtcctcaa atcaaattaa
    21361 aagtcaatag gttgaaataa ctccaggaat ttgctgatat tccgccttca gagggtttgc
    21421 tatgatcagc ggttactgtg atgtgcttaa tgatgctcaa gttaaactcc acgcttgccg
    21481 atagccaacc gcagaatcat gtattgtgtc tggtgcgact gaccacgcct gacagactaa
    21541 gtaagatggg gaaagcatga gcaccattgt gattttttta gctgctttgc tggcctgctc
    21601 actacttgcg ggatggctga taaaagtgcg atccagacgg cgtcagctgc cctggaccaa
    21661 cgccttcgcg gatgcgcaaa cgcgtaaact cacacctgaa gaacgtagcg ccgttgaaaa
    21721 ttatcttgag agcctgacgc aggtattaca ggtgcctggc ccaacgggag ctagcgcggc
    21781 accgatctct ctggcgctga atgccgaaag caacaacgtc atgatgctga cacacgctat
    21841 cacgcgttac ggcatctcta ccgacgatcc gaataaatgg cgttactacc tcgattcggt
    21901 agaagtccac ctgccccctt tctgggaaca atacatcaac gatgagaata ccgttgaact
    21961 gatttatacc gattcgctgc cgctggttat ttcactcaac ggtcatacgc tgcaggagta
    22021 catgcaggaa acgcgcggct atgccttgca acctgttccg tccacgcagg cgtcgattcg
    22081 cggggaagag agtgagcaaa tcgagctact caatattcgc aaagaaacgc atgaagaata
    22141 tgcgctgagt cgtccgcgcg ggctgcgtga agcgttgctg atcgtcgcct ccttcctgat
    22201 gttctttttc tgcctgatta ccccggatgt atttgttccg tggctggcag gcggcgcatt
    22261 actgctgctg ggcgcaggtc tgtgggggct attcgcgccc ccggcaaaat cctccctgcg
    22321 agaaattcat tgtctgcgcg gtacaccccg tcgttgggga ttgtttggcg aaaacgatca
    22381 ggaacagatc aacaatattt cactcggtat tatcgacctg gtctatcccg cacactggca
    22441 gccatacatt gctcaggatc tcggtcaaca aaccgatatt gatatctatc tcgaccgcca
    22501 cgtagtgcgt caggggcgat atctttcgct gcatgatgaa gtaaaaaact ttccgttaca
    22561 gcactggctg cgcagtacga ttatcgctgc gggctcgctg ctggtgctgt ttatgctgtt
    22621 attctggatc ccgctggata tgccgctgaa attcactctc tcatggatga aaggcgcgca
    22681 gaccattgaa gccaccagcg taaaacaact ggctgacgct ggcgtgcggg taggcgatac
    22741 attgcgtatt agcggtacgg gaatgtgtaa tattcgcact tccggaacct ggagcgcgaa
    22801 aaccaattca ccttttttac cgtttgactg ctcgcagatc atctggaatg acgcccgctc
    22861 attaccatta ccagaatctg aactggtcaa caaagcgacg gcattgactg aagcagttaa
    22921 tcgccagctg caccctaaac cggaagatga atctcgcgtc agtgcctcat tacgttcagc
    22981 gattcaaaaa tccggcatgg tattgcttga tgattttggc gacattgtac tgaagacagc
    23041 ggatttatgt tctgccaaag atgactgtgt gcgactgaaa aatgcgctgg tcaatctcgg
    23101 caacagtaaa gactgggacg cgctggtaaa acgcgccaac gccgggaagc tcgatggcgt
    23161 gaatgtgtta ttacgcccgg tgagtgcgga atcgctggat aacctggtgg caacctccac
    23221 cgcgccgttt atcacgcatg aaacggcgcg agcggcacaa tcactaaaca gtccggcccc
    23281 cggcggattc ctgattgtca gcgacgaagg cagcgatttt gttgatcagc cctggccttc
    23341 ggcatcactt tacgactacc cgccgcaaga acagtggaac gctttccaga aactggcaca
    23401 aatgctgatg catacgccgt ttaacgccga aggtatcgtc acaaaaatct tcactgacgc
    23461 caatggtacg cagcatattg gccttcatcc gatcccggat cgttccggcc tgtggcgcta
    23521 tctcagcacc acattgctgc tactgacgat gctgggtagc gccatttaca atggcgtaca
    23581 ggcctggcgt cgttaccagc gtcatcgcac tcgcatgatg gagattcagg cctattatga
    23641 aagctgcctg aacccgcaac tgatcacccc ttcagaaagc cttatcgaat aacacgtttg
    23701 cgcggcaggt tatgctaccc tgtcgcgcaa attgcttcac tctggagatt tccctcatgc
    23761 atatcaacat tgcctggcag gacgtagata ccgttctgct ggatatggac ggcacattgc
    23821 tcgacctcgc cttcgataac tatttctggc aaaagctggt gcctgaaaca tggggcgcga
    23881 aaaacggggt tacgccacag gaagcgatgg aatatatgcg ccagcaatat cacgacgtac
    23941 agcatacgct aaactggtac tgtcttgatt actggagtga gcaactgggt ctggatatct
    24001 gtgcgatgac caccgagatg ggaccgcgtg ccgtactgcg tgaagatacc attccgtttc
    24061 ttgaggcact gaaagccagc ggtaagcagc gaattttgct caccaatgcg catccgcaca
    24121 acctggcggt aaaacttgag cataccggtc tggacgcaca ccttgattta ttactttcca
    24181 cccacacatt tggttatccg aaagaggatc agcggttatg gcatgcggtg gccgaagcta
    24241 cgggtctgaa agctgaaaga acgctgttta ttgatgacag cgaagcgatt ctcgatgctg
    24301 ccgcgcaatt tggtattcgt tactgcctcg gcgtgactaa tcctgattcc gggattgccg
    24361 agaaacagta tcaacgccat ccgtcactga atgactaccg ccgcctgatc ccctcgctaa
    24421 tgtgaaggag acgccatgaa agaaaaacct gctgttgagg ttcgactgga taaatggcta
    24481 tgggctgccc gtttttataa aacccgcgcg ctggcccgtg aaatgattga aggcggtaag
    24541 gtgcattaca acgggcagcg cagcaagccg agcaaaatcg tcgagctgaa tgccacgctc
    24601 actctgcgcc agggaaatga cgaacgcacg gtgattgtaa aggcgattac tgaacagcgt
    24661 cgccccgcca gcgaggcagc cttgctgtat gaagagactg cggaaagtgt agagaaacgc
    24721 gaaaaaatgg cgctggcacg taaacttaat gccttaacca tgccgcaccc ggaccgacgc
    24781 ccggacaaaa aagagcgccg cgacctgtta cgatttaaac acggcgacag tgaataactg
    24841 tcacctgcaa gagagatgat tatgccgcaa catgaccaat tacatcgcta tctgtttgaa
    24901 aactttgccg tgcgcggcga actggtaacc gtttcggaaa ccctgcaaca gatccttgag
    24961 aatcacgatt atccgcagcc cgttaaaaac gtgctggcag aactgctggt tgcgaccagc
    25021 ctgttaaccg ctacgctgaa gtttgatggt gatatcaccg tacagctgca aggcgacggt
    25081 ccgatgaatc tggcggttat taacggtaac aataaccagc aaatgcgcgg tgtggcgcgc
    25141 gtgcagggcg aaattccaga aaatgccgac ctgaaaacgc tggtcggcaa tggttacgtg
    25201 gtgatcacca ttaccccgag cgaaggcgaa cgctatcagg gcgtggttgg tctggaaggt
    25261 gataccctgg cggcctgcct ggaagattac tttatgcgtt ctgaacagct gccgacgcgc
    25321 ctgtttattc gcaccggcga cgtagacggc aaaccggctg caggcggtat gttgttgcag
    25381 gtaatgcctg cgcaaaatgc ccagcaggac gactttgacc acctggcgac gctaaccgaa
    25441 accatcaaaa ccgaagaact gctgacctta ccggcaaacg aagtgttgtg gcgtttgtat
    25501 cacgaagaag aggtgacggt ttacgatccg caggatgtgg agttcaaatg cacctgctcg
    25561 cgtgaacgtt gcgccgatgc gctgaaaacg ctgcctgatg aagaagttga tagcatcctg
    25621 gcggaagatg gcgaaattga catgcattgt gattactgcg gtaaccacta tctgttcaat
    25681 gcgatggata ttgctgaaat ccgcaacaac gcgtctccgg cagatccgca agttcattaa
    25741 tgagtatgtc cggcagagag ggctctgccg gattttacga cgtaatggcc tgatgtgctg
    25801 cgcttaccag gcctgtgaaa taaatcgcaa attattgttg gtcagataca gcgtcgtatc
    25861 cgttaaaaga gttgtcactt acttaagttt atttccagcg tttcaccatc taccgtcaga
    25921 tcgcgccacg ctttcggcct cgtccccagt tttccatcct tcgcagctgt gagtaatttt
    25981 tctttcgtta tttcgttcgg aagtaaactc atatcaagcg cgtcccattc ttttctgtct
    26041 gagtcaaagc catagacaat gactctgtca tcattaaaag caaacaggat ccgctccgcc
    26101 tgaccatcac tattcagatc ttgctcgacc aggacgcagg cgtctttttc aatacaggtc
    26161 atcacgttat agcgatcctg gattaaggcc gaccagaatg tcgcatccgg tttaacagaa
    26221 ccaggggcaa ttaacacatt ttcggctaat actttttccg atacctgttc ctgaagatgt
    26281 tgctctccat ctaacgccat cagcagatcc cgcgcgcgtt tcgggtcttt cataaacccg
    26341 gcatcgcttt tcagcgactc aagcgccgca cgtccatagc gaccgctctg ttcgagcatg
    26401 tagatagtta cctggtctgg cgtgtttttg ccgctctgat aacgcgccat atgactgttc
    26461 acgctaatac gcatactgtc cagcaccggc gaattaagaa gcaccagtat caccaacacc
    26521 aataacgaaa ccgcaaggtt cactttgccc tgaagaacaa ggggattttg cccattacgc
    26581 cagacgatac tgacaaaata ccccagcgac cacaccagta acaccagcac cgccagcgcg
    26641 ccctgcaagc ggtcgacagt ccagccgtac tgagcgaccc gcagccataa cgcccaggcg
    26701 gcgacgaaca catacagcgg agcaaccagc aaagcggttt taatcaggca acgcaacggc
    26761 cccgtccagg gaagtgacgc tttttgcgga tcgcggacaa tagccattaa gatcaattgc
    26821 aaaaaggcca gcgttaacag caacccggcg gcggagatgt ggcgagaaat cgcgctcagg
    26881 cccgtaaacg gcagagtgat gataaacatc agggttagca atgatactaa cggcagcaac
    26941 cccgtggcga ttaacgtgaa caacttttga atagagtcga ttaaacgtga ctgtgtgcgc
    27001 gcgagaatca ccgccagtgc ggtaaccaga cctaatgtaa gatacataaa ccagtcggtt
    27061 gcaaaaaaga gcgtattgaa aaacttgatg ccaacaagtt taaacaactc accccacagt
    27121 aaaagcacca gccacgtcag gccattggcg agaaaaatca ccagtaatat aagtacgtta
    27181 tgccatactg actggtaaaa gtagctatag cggctactgc cattgcagat acgaaggctt
    27241 tgttgtatcc acggtagcaa caacattgcc atcaacagca gatagcagcc aaaatcccaa
    27301 atcgcctttt cagctctcca ggggttcatg ccatcagttt gccacttcag ccaaccgctc
    27361 atccctgtcg tggcaataaa caccagcgcc agccatcccc aaaggcgctt ctgtttaaaa
    27421 gagatcacgg agaagagtaa aacggatgaa aacgcaacgg tcgcaggcac gccataaaca
    27481 atccaactgt gattttttcc ggcaagccag gcgatcaaca ggtagcaaac cagcccctga
    27541 agtaatccgg tagcaatcat cccccagcgt gttgccggtg aaagttcaac gttatccatg
    27601 ttggttatcc agaatcaaaa ggtgggttaa ttatcgcatc cgggcagtag tattttgctt
    27661 ttttcagaaa ataatcaaaa aaagttagcg tggtgaatcg atactttacc gattgaattt
    27721 gcatcaattt cattaaggaa tgcgattcca ctcacaatat tcccgccata taaaccaaga
    27781 tttaaccttt tgagaacatt ttccacacct aaaatgctat ttctgcgata atagcaaccg
    27841 tttcgtgaca ggaatcacgg agttttttgt caaatatgaa tttctccaga tacgtaaatc
    27901 tatgagcctt gtcgcggtta acacccccaa aaagacttta ctattcaggc aatacatatt
    27961 ggctaaggag cagtgaaatg cgcgttaaca atggtttgac cccgcaagaa ctcgaggctt
    28021 atggtatcag tgacgtacat gatatcgttt acaacccaag ctacgacctg ctgtatcagg
    28081 aagagctcga tccgagcctg acaggttatg agcgcggggt gttaactaat ctgggtgccg
    28141 ttgccgtcga taccgggatc ttcaccggtc gttcaccaaa agataagtat atcgtccgtg
    28201 acgataccac tcgcgatact ttctggtggg cagacaaagg caaaggtaag aacgacaaca
    28261 aacctctctc tccggaaacc tggcagcatc tgaaaggcct ggtgaccagg cagctttccg
    28321 gcaaacgtct gttcgttgtc gacgctttct gtggtgcgaa cccggatact cgtctttccg
    28381 tccgtttcat caccgaagtg gcctggcagg cgcattttgt caaaaacatg tttattcgcc
    28441 cgagcgatga agagctggca ggtttcaaac cagactttat cgttatgaac ggcgcgaagt
    28501 gcactaaccc gcagtggaaa gaacagggtc tgaactccga aaacttcgtg gcgtttaacc
    28561 tgaccgagcg catgcagctg attggcggca cctggtacgg cggcgaaatg aagaaaggga
    28621 tgttctcgat gatgaactac ctgctgccgc tgaaaggtat cgcttctatg cactgctccg
    28681 ccaacgttgg tgagaaaggc gatgttgcgg tgttcttcgg cctttccggc accggtaaaa
    28741 ccaccctttc caccgacccg aaacgtcgcc tgattggtga tgacgagcac ggctgggacg
    28801 atgacggcgt gtttaacttc gaaggcggct gctacgcaaa aactatcaag ctgtcgaaag
    28861 aagcggaacc tgaaatctac aacgctatcc gtcgtgatgc gttgctggaa aacgtcaccg
    28921 tgcgtgaaga tggcactatc gactttgatg atggttcaaa aaccgagaac acccgcgttt
    28981 cttatccgat ctatcacatc gataacattg ttaagccggt ttccaaagcg ggccacgcga
    29041 ctaaggttat cttcctgact gctgatgctt tcggcgtgtt gccgccggtt tctcgcctga
    29101 ctgccgatca aacccagtat cacttcctct ctggcttcac cgccaaactg gccggtactg
    29161 agcgtggcat caccgaaccg acgccaacct tctccgcttg cttcggcgcg gcattcctgt
    29221 cgctgcaccc gactcagtac gcagaagtgc tggtaaaacg tatgcaagcg gcaggtgcgc
    29281 aggcgtatct ggttaacact ggctggaacg gcactggcaa acgtatctcg attaaagata
    29341 cccgcgctat tatcgacgcc atcctcaacg gttcactgga taacgcagaa actttcactc
    29401 tgccgatgtt taacctggcg atcccaaccg aactgccggg cgtagacact aagatccttg
    29461 atccgcgtaa tacctacgct tccccggaac agtggcagga aaaagccgaa accctggcga
    29521 aactgtttat cgacaacttt gataaataca ccgacacccc tgcgggtgcc gcgctggtag
    29581 cggctggtcc gaagctgtga tgatttgaag ctggataata tctatccagt atcttagaga
    29641 aagcaaaacg ggagacacct tcgcctcccg ttttatttac ccttcttttg tcgtgccctg
    29701 cgcccgcgtt accggcactg gcagccaggc gcgaatggaa agcccgcccc gctcgctggt
    29761 gccaagctcc agcatcccgt tatggttatc cacgatacgc tgcacaatcg ccagccctaa
    29821 tcccgtgccg ctaatggtgc gcgcactgtc gccgcgaaca aacggctgga aaaggtgctt
    29881 acgctgttcc ggcgcaattc ccggaccgtc atcttccacc tggaaccaag cgcgattcgg
    29941 ctccgtcccg ctgctgactt tgatccagcc attgccgtaa cgggcggcat tgaccaccat
    30001 attcgccacc gcgcgtttga tcgacagcgg gtgcattttc acttcaatgc tgccggggta
    30061 aagcgcggtt tcaatttccc gctcatagcc actttcggca gcaataacct caccgagtac
    30121 tgcgttgaga tccgccattt ccatcggcat ctcctgcccg gtgcgcaggt aatcgataaa
    30181 ctgctcaatg atggcgttgc actcttcgat atctttattg atcgattcgg ccaggtagcc
    30241 atcctgttcg ctcatcatct cggtcgccag gcgaatacgc gtcagcggcg tgcgtaagtc
    30301 atgacttacc cccgccatca gcagcgtgcg gtcatccgcc agttgcttaa caccagccgc
    30361 catatggtta aaggcgcggg taaccgaacg cacctccgaa gcgccatact cacgcagcgg
    30421 cggcggaata atccctttac cgacctgcaa agcagcatgt tcgagatcga ccaacggtcg
    30481 gttctggata cgaataaaca gccacgcccc gcctatcgcc aatagcataa tcgccaacgt
    30541 atagcggaac agcggggaaa aatcgccctg atgaatttcg gtcagcggca cccgtaccca
    30601 gatattgggc gacagccagg ttttcagcca gacgacaggc gaacttttgt tgacctcaac
    30661 gcgcacttcc gtcgggccgc ccagttgctg tgccatctga tggcttaaga attcatagtg
    30721 ttgtgcccaa cgcagacctg cctcttcggc agcttcgttg gagtagagag agatccccag
    30781 ctcacggtag atctcccgac ggaaagcggg aggcacaacc aactgcgtgc cgtcctccag
    30841 ttgcagtttg tcggtcatca acatacgcac ttcgtacgcg aggactttat taaactgctg
    30901 gaggctcggc aaaatcgcga agttcagcac caccagataa gtcgtcacca ggctggcgaa
    30961 cagcaaggtg acgatgagca ataacgtacg ggcaaatgaa cttcgtggcg agaagcgcaa
    31021 tcgcctcatg ctttagagcc gtccggtaca aagacgtagc ccaggcccca aacggtctga
    31081 atgtaacgcg gatgcgccgg atcttcttcc accatgcggc gcagacgtga aatctgcacg
    31141 tcgatggagc gttccattgc ggaatattca cggccacggg caaggttcat cagcttatcg
    31201 cgggagagcg gctcacgcgg atggctgacc agtgccttca gtaccgcaaa ctcaccgctg
    31261 gtgagcggca tcggctcatc ttcgcggaac atttcgcgcg taccgaggtt aagtttgaac
    31321 ttaccgaaag caattaccgc ctcttcctgt gacggtgcgc ctggtagctc gttcgcctga
    31381 cgacgcagca ccgcacggat acgggccagc agttcacgcg ggttaaacgg ttttggaatg
    31441 taatcgtcag cgccaatctc caggccaacg atacggtcca cttcttcccc tttcgccgtt
    31501 accataatga tcggcatcgg gttgctctgg ctacgaagac gtcggcaaat cgacaagcca
    31561 tcttcaccag gtaacatcaa atccagtacc ataagatgga aagattcacg agtcagcagg
    31621 cgatccatct gttctgcatt agcgacgctt cgaacctgga agccttgttc ggtgagataa
    31681 cgttccagca gcgcacgcag gcgcatgtcg tcatcgacca ccagaatctt gtagttctct
    31741 tgcattgttt gtactcccaa aggttcgcaa caatttgtaa gcgtgtattc ttaaaaaagc
    31801 tcacgttcgt caccagctaa atctggtatg aatttcagcc taaattgtta caaagcatat
    31861 taaacagcag cttaagtata caatttattc ggcgaaacat tattgattct gttgatatga
    31921 tcacgttata cccaatgtgc gcattatcaa acagacaaag ggaatcaacg agatgaaaac
    31981 gcccctggtt acccgggaag ggtatgaaaa actcaaacaa gagcttaatt atctctggcg
    32041 tgaagaacgc ccggaggtca caaaaaaggt gacctgggcc gcaagtctgg gcgaccgcag
    32101 cgaaaatgct gactatcagt ataataaaaa gcgtctgcgt gaaatcgacc gtcgcgtgcg
    32161 ctatctcact aaatgcatgg aaaatctcaa aatcgtcgat tactcccctc agcaggaagg
    32221 caaagtcttt tttggcgcgt gggtggagat tgaaaacgac gatggcgtga ctcaccgttt
    32281 ccgtattgtc ggctacgatg aaatttttgg ccgtaaagat tacatctcta tcgattcccc
    32341 gatggcccgc gcattgctga aaaaagaagt cggcgatctg gcggtggtga atacccctgc
    32401 cggggaagcg agctggtatg ttaatgctat cgagtacgtg aaaccgtaag gaagagtctt
    32461 aacctcctgc cgatggctgg catttttgcc agccagtccg tataactatc ccctgatttt
    32521 tgatccgaaa agatgaactc aaaccatgat gaatgattcg ttctgccgca ttattgcggg
    32581 tgaaattcag gcgcgcccgg aacaggttga cgctgccgtt cgcctgcttg acgaagggaa
    32641 taccgtgccg tttatcgcac gttatcgtaa ggaaatcacc ggcggtctgg atgacacgca
    32701 actgcgtaat ctggaaacgc gtctgagcta tctgcgcgag ctggaagaga gacgtcaggc
    32761 gatcctcaaa tccatttccg agcaaggcaa actcaccgat gatctggcga aggccatcaa
    32821 cgccacccta agcaaaaccg aactcgaaga cctctacctg ccctacaaac ctaaacgccg
    32881 cacccgcggg caaatcgcca ttgaagcagg gcttgagccg ttggctgacc tgctgtggag
    32941 cgatccgtca cacacgccag aagtcgccgc tgcacaatat gttgatgccg ataaaggcgt
    33001 ggcagatacc aaagccgcgc tggatggcgc gcgctatatc ctgatggaac ggtttgccga
    33061 agatgccgcg ctgctggcga aagtgcgtga ttatctgtgg aagaacgcgc atttggtttc
    33121 tacggtggtg agcggtaaag aagaggaagg ggcgaaattc cgcgactatt tcgatcatca
    33181 cgaaccgttg tccacggtgc cttctcaccg cgcgctggcg atgttccgtg ggcgtaacga
    33241 aggcgtactc cagctttcgc tgaatgccga tccgcagttc gatgagccgc ccaaagagag
    33301 ctattgcgag caaatcatca tggatcacct tggcctgcgc ctgaacaatg ccccggcgga
    33361 tagctggcgc aaaggcgtag tgagctggac ctggcgcatc aaggtgctga tgcatctgga
    33421 aaccgaactg atgggtaccg tgcgcgaacg cgcggaagat gaagcaatca acgtctttgc
    33481 ccgtaacctg cacgatctgc tgatggcggc ccctgccgga ctgcgtgcaa cgatgggcct
    33541 cgatccgggt ctgcgtactg gggtaaaagt ggcggtggtc gatgccactg gcaaactggt
    33601 ggcgaccgat accatttacc cgcacaccgg acaagccgca aaagcagcga tgaccgttgc
    33661 tgccttgtgt gaaaaacata acgttgaact ggtggcgatc ggcaacggta cagcttcccg
    33721 cgaaactgaa cgtttctatc tcgacgtgca gaagcagttc ccgaaagtga ccgcacagaa
    33781 agtgatcgtc agcgaagctg gcgcgtcggt ttactcagct tccgagctgg cagcgcagga
    33841 gttcccggat ctcgacgttt cgctgcgtgg tgcggtatct atcgcccgcc gtttgcagga
    33901 tccgctggcg gagctggtga aaatcgatcc gaaatctatc ggcgtaggtc agtatcagca
    33961 tgacgtcagc cagacgcaac tggcccgcaa actggacgca gtagtagaag actgcgtaaa
    34021 cgccgttggc gtcgatctca acactgcttc tgttccgctg ttaacccgcg tggcgggcct
    34081 gacgcgcatg atggcgcaaa acatcgtggc ctggcgcgat gagaacggtc agttccagaa
    34141 ccgtcagcaa ctgctgaaag tgagccgtct ggggccgaaa gccttcgagc agtgcgcggg
    34201 cttcttgcgc attaaccatg gtgataaccc gctggacgcg tctaccgtcc acccggaagc
    34261 ctatccggtg gtggaacgca ttctggcagc aacacagcag gcactgaaag atctgatggg
    34321 taacagcagc gaactgcgta acctgaaagc gtctgacttt actgatgaaa aattcggtgt
    34381 gccgacagta actgacatca tcaaagagct ggaaaaaccg ggtcgcgatc cgcgtccgga
    34441 atttaaaacc gctcagtttg ccgatggcgt cgagacaatg aacgacctgc aaccgggtat
    34501 gatcctcgaa ggtgcggtga ccaacgtcac caactttggc gcgtttgtcg atatcggcgt
    34561 gcatcaggac ggactggttc acatctcttc attgtcgaac aagtttgtag aagatccgca
    34621 taccgtggtg aaagcgggcg acattgtgaa ggtgaaagtg ctggaagtgg atcttcagcg
    34681 taaacgtatc gccctgacca tgcgtctgga cgagcagcct ggcgaaacca acgcccgtcg
    34741 cggcggcggt aatgaacgcc cgcaaaacaa ccgcccggca gccaaaccgc gcggacgtga
    34801 agcgcagcct gccggtaaca gcgcgatgat ggacgcgctg gcggcagcaa tgggcaaaaa
    34861 acgttaaacg ccctgattga cccctaaacc tgcgcgataa ggtgtccacg ccgcatctgg
    34921 caacgaacgc cttgtctgac ataaaagtgc cggagaatat ctccggcatt tttattccac
    34981 agccaaactc acaatatatt ccggcaacat ttatcacttc attaacaact gaaaccttaa
    35041 ttaaacatta gccagtcctg gtaattcgct attcgaatta tattttcgct gcgatataac
    35101 cttgagccac atcaacattg agtcagatta ttattcaaac caacattcgc acacatttta
    35161 agtattgctg atagaaacca ttctcattat cattgtgctg ttgattattt aatctctcct
    35221 tcgttggcaa atcatctggt ctcatgtcgc tgtcaaacgc cccatgaggt agttatccag
    35281 ttaatgagaa acaagtaggc acctatgcaa tacactccag atactgcgtg gaaaatcact
    35341 ggcttttccc atgaaatcag cccggcatat cgccaaaaac tgctttctct tggcatgtta
    35401 cctggctcct cttttaatgt ggtgcgcgtc gctccactcg gcgaccccat tcatatcgaa
    35461 acccgtcgtg tgagcctggt attacgcaaa aaagatctgg ccttattaga agtggaagcg
    35521 gtttcctgtt aatacggtga taacaacaat gaaaaaatta accataggct taattggtaa
    35581 tccaaattct ggcaagacaa ccttatttaa ccagctcact ggcgcacgtc agcgtgtagg
    35641 taactgggct ggcgttaccg tcgaacgtaa agaagggcaa ttctccacca ccgatcatca
    35701 ggtcacgctg gtggacctgc ccggcaccta ttctctgacc accatctcat cgcagacctc
    35761 gctcgatgag caaatcgcct gtcactacat tttgagtggc gacgccgacc tgctgattaa
    35821 cgtggtggat gcgtctaacc ttgagcgtaa cctgtacctg acgctacaac tgctggaact
    35881 cggcattccc tgcatcgtgg cactgaacat gctcgacatt gccgagaagc aaaatattcg
    35941 tattgaaatt gatgctctgt cggcgcgtct tggctgccca gtgatcccgc tggtttcaac
    36001 ccgtggtcgc ggtattgaag cgctcaagct ggcgattgat cgctataaag ctaacgagaa
    36061 tgtggaactg gtgcattacg cacagccgct actcaacgaa gcagattcac tggcaaaagt
    36121 gatgccttcc gacatcccgc tgaaacaacg tcgctggctg ggcctgcaaa tgctggaagg
    36181 cgatatctac agccgcgcct acgccggtga agcgtcgcag catctggatg ccgccctcgc
    36241 ccgtctgcgt aatgagatgg acgatccggc gctgcacatt gccgatgcgc gttaccagtg
    36301 cattgctgcc atctgtgatg tggtaagcaa caccctgacg gcagaaccca gccgtttcac
    36361 cactgcggta gataaaatcg tgctcaaccg tttcctcggt ctgccgattt tcctctttgt
    36421 gatgtacctg atgttcctgc tggctatcaa catcggcggg gcgttacagc cgctgtttga
    36481 cgtcggctcc gtggcgctat ttgtgcatgg tattcaatgg attggctaca cgctccactt
    36541 cccggactgg ctgactatct tcctcgccca gggcctgggt ggcggtatta acaccgtgct
    36601 gccactggtg ccgcagattg gcatgatgta cctgttcctc tccttccttg aggactccgg
    36661 ctatatggcg cgtgcggcgt ttgtgatgga ccgcctgatg caggcgctgg gcttgcctgg
    36721 gaaatccttt gtgccgctga tcgtcggttt cggttgtaac gtaccgtcgg taatgggcgc
    36781 acgtacgctt gatgcaccgc gtgaacgtct gatgaccatc atgatggcac cgtttatgtc
    36841 ctgcggcgcg cgtctggcta tcttcgcagt attcgcggct gccttcttcg ggcagaacgg
    36901 tgccctggcg gttttctcgc tgtatatgct gggtatcgtg atggcggtgc tgactggcct
    36961 gatgctcaag tacaccatca tgcgcggtga agcgacgccg tttgtcatgg agctgccggt
    37021 ctaccatgta ccacacgtta aaagcctgat tatccagacc tggcagcgtc tgaaaggctt
    37081 cgttctgcgt gctggtaaag tgattatcat cgtcagcatt ttcctgagcg ccttcaacag
    37141 cttctcgctg agcggaaaaa tcgtcgataa catcaacgac tcggcgctgg cttccgtcag
    37201 ccgggtgatc accccagtct tcaagccgat tggcgtgcat gaagataact ggcaggcaac
    37261 ggttggcctg tttacaggtg caatggcgaa agaagtggtg gtgggcacgc tcaacaccct
    37321 ctacaccgca gaaaatattc agtacgaaga gttcaatccg gcagaattta acctcggtga
    37381 agagctgttc agcgcggtag atgaaacctg gcagagcctg aaagacacct tcagccttag
    37441 cgtactgatg aatcccattg aagcgagcaa aggcgacggc gaaatgggta ccggggcgat
    37501 gggcgtgatg gatcagaaat tcggtagcgc agctgccgca tacagctacc tgattttcgt
    37561 cctgctgtat gtaccatgta tctcggtgat gggagccatc gcccgtgaat caagccgtgg
    37621 ctggatgggc ttctccatcc tgtgggggct gaatatcgct tactcactgg caacattgtt
    37681 ctatcaggtc gccagctaca gtcagcaccc aacttacagc ctggtgtgca ttctggcggt
    37741 aatcctgttt aacatcgtag ttatcggtct gctgcgccgc gcgcgtagcc gggtggatat
    37801 cgaactgctg gcaacccgca agtcggtaag cagttgctgc gcagccagca ccaccggtga
    37861 ttgccattaa tggcttcact tattcaggta cgtgatttgc tggcgttacg gggccgtatg
    37921 gaagcgaccc agataagcca gacattgaac actccgcagc caatgattaa cgccatgctg
    37981 caacagctgg aaagcatggg caaagccgtg cggattcagg aagaacctga cggctgcctc
    38041 tctggcagtt gtaaaagctg cccggaaggc aaagcctgtc tgcgcgagtg gtgggcgctg
    38101 cgttaaccat actccatcgc ctgttttgaa aagcccggta tgcgtttgca tccgggcttt
    38161 tttgcgtgcg gctttccata aaaatgcaat tcttgcagcg cgacgtaagt tcctctgaaa
    38221 gcgtctcgca gggatgaaaa ctggctaatg cacagatgtg gagtggcgcg tagagtcgcg
    38281 gcattcaaac aacaggtgaa ggaacgccat gagtaaaaag cagagttcca ccccacatga
    38341 tgcgctgttc aaactctttt tacgccaacc ggagacggca cgcgattttc ttgcgtttca
    38401 tttaccggca cccattcacg cgctctgtga tatgaaaacc ctcaagctgg agtcgagcag
    38461 ctttattgat gacgatctgc gtgaaagcta ttccgatgtg ctgtggtcgg tgaaaacgga
    38521 acaaggacca ggatacatct attgtctgat tgaacatcaa agcacctcaa acaaactgat
    38581 cgcatttcgc atgatgcgtt acgctattgc cgcaatgcaa aatcaccttg atgctggata
    38641 caaaacgttg ccgatggtgg tgccattgtt gttttaccac ggtattgaaa gcccctatcc
    38701 ctattcgctg tgttggctgg attgtttcgc cgatcccaaa ctggcaaggc aactttatgc
    38761 ctccgcattt ccgctgattg atgtcaccgt catgcctgat gatgaaatca tgcagcaccg
    38821 acgtatggcg ctgctggagt taattcaaaa acatattcgt caacgcgatc tgatggggct
    38881 ggtagagcaa atggcctgct tattaagtag tggatacgct aatgacagac aaatcaaagg
    38941 gctgtttaat tacatactgc aaactggcga cgctgtacgt tttaacgatt ttatcgacgg
    39001 cgttgccgaa cgttcaccga aacacaagga gagtttaatg actattgcgg aaagattgcg
    39061 gcaggagggg gaacaatcca aagccctgca tatagccaaa ataatgcttg aatccggagt
    39121 ccctcttaca gacatcatgc gctttaccgg ggtgtcagaa gaagagttgg ctgcggcgag
    39181 ccagtaaatt tctgtctcgc catttcaaaa gttgcctaca ccctctgctt caacgccacc
    39241 agcaggtgac aaaactcgac cggatgcgaa ataaacggcg catgggccgc tttggcgaag
    39301 atatatgatt cgctgtgagg ccaaagttta tccagcatcg gcaccacttt gcgcggcacc
    39361 agaccgtcga gatagccata caatcgcaaa aacggcatgg acacgttttg cagcggctga
    39421 cggagatcga ccgttttcag gatttccagc ccgccattaa gcacgtcaac ctccggcatc
    39481 ggtaacgcca gaacggtttt cttcaacgcc cgcgcatcct ggcgcgccgt ttcagtcccc
    39541 atggtttgta acgccaggaa ccgctccact gtacgctgaa aatcatcact gagttgctgc
    39601 tgaaatcccg ccagcacgtc cggttttatc cccggccact cgtcacgagc actaaaacaa
    39661 ggtgacgacg ccacggtgac cagcgcctga acacgctcgg gatgggttaa cgcaatctgg
    39721 cttgccacca gcccgcccag actccagcct aaccaaatgg ctttatcagg tgcctgttgc
    39781 agcacggctt cggccatatc agcaagtgac agcgcaccaa atccccggct acgcccgaag
    39841 ccgggcaggt caacaaggtg cagcgtaaaa tgcgagctaa gttcctcgtc aatgcaacac
    39901 cacacttcgg cattcagtcc ccatccgtgc agcagcacaa gatgaacatt cccctgacct
    39961 ttggtctgcc accagatgtt attcatccgc tattgttctc ttttgactta caaggatgaa
    40021 catatgctaa cagtaccggg attatgctgg ctatgccgaa tgccactggc gttaggtcat
    40081 tgggggattt gttcggtctg ttcacgcgcc acccgcacag ataaaacgtt atgcccacaa
    40141 tgtggattac ccgccacaca ctcccatctt ccctgcggtc gctgcctgca aaaaccgccg
    40201 ccctggcaaa gactggtcac ggttgccgac tatgcgccgc cgttaagtcc gcttatccac
    40261 cagcttaaat tttcccggcg cagtgaaatc gccagcgccc tgtcacgtct gttactattg
    40321 gaagtcttac acgctcgtcg caccaccggg ttgcaattgc cggatcgcat cgtcagcgtt
    40381 ccgttatggc agcggcgtca ctggcgtcgg ggatttaatc agagcgattt gctgtgtcag
    40441 ccgttatcac gctggttgca ctgccaatgg gatagcgaag ccgtcacacg tacacgggcc
    40501 actgcgaccc agcattttct cagtgcccgg ctgcgcaagc gcaacctgaa aaatgccttt
    40561 cgtcttgaat tgcccgtgca aggtcgccat atggtgattg tggatgatgt cgttaccacc
    40621 ggaagtaccg tcgcagagat tgcgcagttg cttttacgca atggtgcggc ggctgtccag
    40681 gtctggtgcc tttgtcgaac cttgtagagc ctcgatgatg ggcgtattat aaccaactaa
    40741 aatagtcaac tattaggcca ttactatgat ccgtatttcc gatgctgcac aagcgcactt
    40801 tgccaaactg ctggcaaatc aggaagaagg gacacaaatc cgcgtatttg tgattaaccc
    40861 tggcacgcct aacgctgaat gtggcgtttc ttattgtccg ccggacgctg tggaagccac
    40921 cgacacagcc ctgaaatttg acctgctgac cgcgtatgtt gatgagttaa gcgcaccata
    40981 cctggaagat gcagagatcg actttgttac cgaccagttg ggttctcagt taacgctgaa
    41041 agccccgaac gccaaaatgc gtaaagtggc agacgatgca ccgctgatgg agcgcgtgga
    41101 gtatatgctg cagtcgcaga tcaacccaca gcttgctggt cacggtggtc gcgtttcgct
    41161 gatggaaatc accgaagacg gttacgccat tctgcaattt ggcggcggct gtaacggttg
    41221 ttccatggtc gatgtgacgc tgaaagaagg gatcgagaag cagctgctga acgaattccc
    41281 ggagctgaaa ggtgtgcgcg atctcaccga acaccagcgc ggcgaacact cctactacta
    41341 agttatcccc tcttttgtag attgcccgat gcgacgctaa agcgtcattt cgggcctacg
    41401 ttgaacatgt gccagacgtt ggcgcagcat tgctttggca aatccctccc ttcgtttctc
    41461 cgcgataata tgaccaacct ctcataattt aaatttaccc cgctctggtg attctcaaac
    41521 gccagatgtt acccgtatca ttcacatggg taccaaacat actcctgaca tctgactaca
    41581 ataattagtt ttagtgggta tcagtcgtgg tgccgcaata tctctgttcc cgattgggat
    41641 aattagagtt tgtcgtcaga aaattgacgt tacccataac aaatgaaagg ccaggtaaat
    41701 catgccatta gtcattgttg ctatcggtgt aatcttgttg ttgctcctga tgatccgctt
    41761 caaaatgaac ggcttcatcg ctctcgtcct cgtggcgctt gctgttggat taatgcaagg
    41821 aatgccgctg gataaagtta ttggctccat caaagccggt gtcggcggga cgctcggtag
    41881 ccttgccctg atcatgggtt ttggcgcaat gctgggcaaa atgctggcag actgcggtgg
    41941 cgcacaacgt atcgccacca cgctgattgc caaatttggt aaaaagcaca tccagtgggc
    42001 ggtggtgtta accggtttta ccgttggttt tgccctgttc tatgaagtgg gcttcgtgct
    42061 gatgctgcct ctggtgttta ccatcgcggc ttccgcgaat attccactgc tgtatgttgg
    42121 tgtaccaatg gcggctgcac tgtctgtgac ccacggcttc ctgccaccgc atccgggccc
    42181 gactgcgatt gccaccattt tcaatgccga tatgggtaaa accctgctgt acggtactat
    42241 tctggcaatc ccgaccgtga ttctcgccgg tccggtttac gctcgcgtgt tgaaaggtat
    42301 cgataagcca atcccggaag gtctctacag cgcgaaaacc ttcagcgaag aagagatgcc
    42361 gagctttggc gtcagcgtct ggacttctct ggtgccggtt gtgctgatgg cgatgcgtgc
    42421 gattgccgaa atgatcctgc cgaaaggtca cgctttcctg ccggtagcgg agttcctcgg
    42481 tgacccggta atggcaacgc tgattgccgt gctgattgcg atgttcacct ttggtctgaa
    42541 ccgtggtcgt tcaatggatc agattaacga cacgctggtt tcttccatca aaatcattgc
    42601 gatgatgctg ttgatcatcg gtggtggcgg tgcgttcaag caggtgctgg tagacagcgg
    42661 cgtggacaaa tacattgctt ccatgatgca cgaaaccaac atttctccgc tgctgatggc
    42721 ctggtcgatt gctgccgtac tgcgtatcgc gctgggttcc gcaaccgttg cggcaatcac
    42781 tgcgggtggt atcgcggcac cgctgattgc aacgacgggt gttagcccag agctgatggt
    42841 tattgcggtg ggttccggta gtgtgatttt ctctcatgtg aacgatccgg gcttctggct
    42901 gttcaaagag tactttaacc tgactatcgg cgagaccatc aaatcctggt cgatgctgga
    42961 aaccattatc tcggtgtgcg gtctggtagg ctgtctgctg ctgaatatgg tgatttgata
    43021 acactgactg ccggatgcat cgcatccggc atcaaccgca ctctacttct tcttcgctgc
    43081 agctctgcgc cgtctgtcca aatccttcag caacttgttc acgccatcat cggcaaacat
    43141 cgtctcaagc gttgcggaaa gcttgcgtcg ccagttttta tactggtaac tggtgccagg
    43201 aatattcacc ggttcggcca tatccagcca gtcttccggc tgtagtccta acagagcact
    43261 gttactgtcg gcaatgtagc gctgcaaacc acggttcagc gtcggcgtca tcgacatcaa
    43321 cgatgcctta tgcccggcac gttttggcag acaaccatat ttatgcagtg catccagcag
    43381 cccttgcttc gccagttcgc gatcctgata cagaccgcgc agtaccactt catccggata
    43441 cagccccagg gttttgccca gcgttagatc cccgctctcc cagtaaccgc gtagcgtagg
    43501 caggtcatgt gtcgccgcaa ccgccatcga ctgctccgga tacgctttcg gtgcacggaa
    43561 cgtcttctcg tggtcgtttt cgaaatagag cactttgtag gagtacacac cgctgctgcg
    43621 cagcttaccg acaatctcta ccggtacggt accgagatct tcaccaatca ccatacagcg
    43681 atgacgttta ctttcgagtg ccagaatcga gagcagatca tccaccggat agtgaacata
    43741 cgcgccctga tctgccgtct cgccatacgg tatccaccac aaacgcagca tcgacatcac
    43801 atggtcaatt cgtaatgcgc cgcagttttg catattggca cgcaacagct cgataaacgg
    43861 ttcataggca cgcgcggtga tgatatgcgg gtccattggc ggtaatcccc agttctgccc
    43921 caacgggccg aggatatccg gcggcgcgcc aaccgacgct ttcaggcaat atagttcacg
    43981 gtcacaccag gtttccgccc caccttccgc tacgccaacc gccagatcac gatacaagcc
    44041 aatcggcatt tcatagccct ggcttatctc ccagcaggcg gcaaactggc tgtaagccag
    44101 ccactgcaac cagagataaa aatcgacgtc attacgatgt tcttcgcaga actgacgcac
    44161 ttctggtgaa tccacgttct gatacatctc tggccatgca ggccagcccc agcgcatttc
    44221 gtcctctttc acttgctggg catgtagcgc atcaaaggct gcctgccaga acaggctgtc
    44281 gccctgctct gcaacaaact ggcgaaacgc ggtcatctgc tcatcatcac gttgcgcgaa
    44341 acctttccac gccattcgta atgctgtcat ttttagggcg gtaaccgtgg agtaatcgac
    44401 ccagtcggca tcgcgcgcct gttgcagcgt ctgttgcgtg gtcggcaact gccaccaggc
    44461 ctgagcctct tcgctaagat ggaaatcttc aacggcgtta acgtcgatat aaatcacatt
    44521 cagccaacgg cgagaagacg ggctgtatgg gctggcactc tccggatttg ccggatagag
    44581 cgcatgaatc gggttcaggc cgatgaacga cccgccacgt tttgccacat ccaccagcat
    44641 cgctttgaga tcgccaaaat ccccaatacc ccagtttttt tccgatcgca gcgtataaag
    44701 ctgaacgcag gcaccccaca gcttttgttt attcagcaac gcctgcggtt cgtaacagcg
    44761 tttcggggcg acaatcaccc ggcaatgcgc gcgctggtcg tcctgggtga gtgtcagcgt
    44821 gtgataacct tccggcagct tcgtcggtag attgaacgct ttgccccccg ttacatggcc
    44881 tttgtactgt gttccttctt cggtggtcag cagccagcta tattcgccgc tgccctccac
    44941 caccatcggc atttttttgc cgctggtata aaccatgaca ttcgggactg gcgttaccgc
    45001 cactttcgtg gcggtacgtt gatgcatcgc gtcaagcaaa cgccgtttgg tttcggcgct
    45061 aatcgactgc ggtttaccgt gggcattgat gtaattgggg ctaatccccg ccgccagcgc
    45121 ggcattatcc agacgtttgc tttccatcga gcttccttag cgttttgcct gccagatacg
    45181 agcctgataa tcgcgaatag agcgatccga gctaaacata ccgcagcggg cggtattgag
    45241 gatcgccgcg cgagtccagg cctcctggtc gcggtacagc acatccacct gcttttgtgc
    45301 ctcaacatag gctgcgaaat ccgccatcac cagatacgga tcgccgcctt gtttgccgat
    45361 actgtgcagc atctggtcga aggcatgctt atcgccgtcg ctgtatttac cgctttccag
    45421 ctctttcaat actgcgtcca gcaccttatc tttcttccgc catttcaccg ggtcgtagcc
    45481 tttggccaga atagccttca cttgttccac ggtatgacca aaaataaaga tattttcttc
    45541 accgactttc tcggcgattt caacgttcgc accatccagc gtaccgacag taagcgcacc
    45601 attgagcgcc agtttcatat tgccggtacc ggaagcttct ttacctgcag tcgaaatttg
    45661 ttcggagata tccgccgccg ggatcagttt ttccgccgcc gaaacgcaat aatccggcag
    45721 gaacaccacc ttcaacttat cgccaaccag tggatcgttg ttgatcacgt cagccacttt
    45781 gttgatcgca aagataatat tcttcgccag gtagtagccc ggtgccgctt tcgcgccgaa
    45841 gaggaagacg cgcggtacgc gatcagcctg cgggttttca cgaatttctt tgtacaacgc
    45901 cagaatatgc agcagattca ggtgctggcg tttgtactca tgcagacgtt tgatctgaat
    45961 atcgaaaatc gcctgtggat tgatctcaat accggtacga actttcacaa actccgccag
    46021 acggacttta ttcgcctgct tgatctcgcg atattgctga cggaatttcg catcatcagc
    46081 gaatttttcc agattgatca gctgatcgag atcgttagcc cactcttttt gcagtgattt
    46141 atccaacaga gccgccagtg cagggttgca ctgtttgatc cagcgacgtg gggtaatacc
    46201 gttggtgacg ttatggaatt tgttcggcca tagctggtga tattccggga acagatcttt
    46261 caccaccaga tccgagtgca gcgccgcaac accgttcacc gcgaaaccgc caaccacaca
    46321 caggttcgcc atatgcactt gtttatcgtg caccaccgcc agtttggccc acactttttc
    46381 atcgcccggc caggttttct ccaccagcgt tttaaagcga gtattaattt cgttaataat
    46441 ctgcatatgg cgcggcagta agcctttcac cagtttcaca tcccagcgtt ccagcgcttc
    46501 tggcatcagg gtatggttgg tgtaagcgaa agttttgctg gtaatggccc aggcgtcatc
    46561 ccagctcatc tggtgctcat cgatcagcac gcgcagcagt tctggaatcg cgatagttgg
    46621 gtgggtatcg ttcagctgaa taacttcgta atccgccagt tcgtgcagtt tacgccccgc
    46681 cagatgatgg cgacgcaaaa tatccgctac cgaacaggca cactggaagt actgctgcat
    46741 caagcgcagc tttttaccgg cagtatggtt gtcgtttgga tagagaactt tggtcagttt
    46801 ttccgcattg atgccctgct gttcggcacg caggaaatca ccgtcgttaa atttagtcag
    46861 atcaaacgga tgcgcgtgcg tcgcctgcca cagacgcagc ggctgcgcca cgccattacg
    46921 atagccgaca acggggagat cccacgcttg accggtaatg gtaaactccg gctcccagcg
    46981 tccgtctttc gtcactttac cgccaatccc tacctgcaca tccagtgctt cgttgtggcg
    47041 gaaccacggg tagttactgc gatgccagtc atccggcgct tcaacctgtt tgccatcgac
    47101 aaaagactgg cggaacaaac catattgata gttcagaccg taacccgtcg cagactgacc
    47161 gacagttgcc attgagtcga ggaagcacgc cgccagacgt cccagaccac cgttacccag
    47221 cgccgggtcg atctcttctt ccagcaggtc cgtcagattg atgtcataag ccttcaacga
    47281 atcctgtaca tcctgatacc agccgagatt caacaggttg ttgcccgtca ggcgaccaat
    47341 caaaaactcc attgagatgt agttaacatg tcgctgattc gccaccggct tggcgaatgg
    47401 ctgagcacgc agcatttcgg ccagtgcttc actcactgct agccaccact ggcgaggagt
    47461 catttcagcc gcagaattta agccataacg ctgccactga cgtgaaagcg cttcctgaaa
    47521 ttgcttatcg ttaaaaatag gttgtgacat aggagttcca cttttcttag attttcaaca
    47581 caacgttatc gctagtttgc caggctcgat gttgaccttc ctcatcctgc gggggattag
    47641 gcagggagga gttgcgggga tgagcaagga aatgtgatct caaccactta aagctagtgc
    47701 aaaccacagg attagcagca aatcaatgca atacagcgca gaaaatctgt atctaagtgc
    47761 aaaaaatggc cgttgcgtat tttcaaaaag cggaaggtaa ctctataaat taagtaaagg
    47821 agtgaaacag tttcataagt aaaatatcca gtgtgctcca tctcattctt aatagattta
    47881 ttaagatcat ctttttagat ggcactttca tcaggaatga agaagaaacc cttgcttaaa
    47941 tgaatctgat gaacataagg gaaaccagta ttcacgctgg atcagcgtcg ttttaggtga
    48001 gttgttaata aagatttgga attgtgacac agtgcaaatt cagacacata aaaaaacgtc
    48061 atcgcttgca ttagaaaggt ttctggccga ccttataacc attaattacg aagcgcaaaa
    48121 aaaataatat ttcctcattt tccacagtga agtgattaac tatgctgatt ccgtcaaaac
    48181 taagtcgtcc ggttcgactc gaccataccg tggttcgtga gcgcctgctg gctaaacttt
    48241 ccggcgcgaa caacttccgg ctggcgctga tcacgagtcc tgcgggctac ggaaagacca
    48301 ccctcatttc ccagtgggcg gcaggcaaaa acgatatcgg ctggtactcg ctggatgaag
    48361 gtgataacca gcaagagcgt ttcgccagct atctcattgc cgccgtgcag caggcaacca
    48421 acggtcactg tgcgatatgt gagacgatgg cgcaaaaacg gcaatatgcc agcctgacgt
    48481 cactcttcgc ccagcttttc attgagctgg cggaatggca tagcccactt tatctggtca
    48541 tcgatgacta tcatctgatc actaatccag tgatccacga gtcaatgcgc ttctttattc
    48601 gccatcaacc agaaaatctc accctggtgg tgttgtcacg caaccttccg caactgggca
    48661 ttgccaatct gcgtgttcgt gaccaactgc tggaaattgg cagtcagcaa ctggcattta
    48721 cccatcagga agcgaagcag ttttttgatt gccgtctgtc atcgccgatt gaagccgcag
    48781 aaagcagtcg gatttgcgat gacgtttccg gttgggcgac ggcactacag ctaatcgccc
    48841 tctccgcccg gcagaatacc cactcagccc ataagtcggc acgccgcctg gcgggaatca
    48901 atgccagcca tctttcggat tatctggtcg atgaggtttt ggataacgtc gatctcgcaa
    48961 cgcgccattt tctgttgaaa agcgccattt tgcgctcaat gaacgatgcc ctcatcaccc
    49021 gtgtgaccgg cgaagaaaac gggcaaatgc gtctcgaaga gattgagcgt caggggctgt
    49081 ttttacagcg gatggatgat accggcgagt ggttctgcta tcacccgctg tttggcaact
    49141 tcctgcgcca gcgctgccag tgggaactgg cggcggagct gccggaaatc caccgtgccg
    49201 ccgcagaaag ctggatggcc cagggatttc ccagcgaagc gattcatcat gcgctggcgg
    49261 caggcgatgc gctgatgctg cgcgatattc tgcttaatca cgcctggagt ttgttcaacc
    49321 atagcgaact gtcgctgctg gaagagtcgc ttaaggccct gccgtgggac agtttgctgg
    49381 aaaatccgca gttagtgtta ttgcaggcgt ggctaatgca aagccaacat cgctatggcg
    49441 aagttaacac cctgctagcc cgtgctgaac atgaaatcaa ggacatcaga gaaggcacca
    49501 tgcacgcaga atttaacgct ctgcgcgccc aggtggcgat taacgatggt aatccggatg
    49561 aagcggaacg gctggcaaaa ctggcactgg aagagctgcc gccgggctgg ttctatagcc
    49621 gcattgtggc aacctcggtg ctgggtgaag tgctgcactg caaaggcgaa ttgacccgct
    49681 cactggcgct aatgcagcaa accgaacaga tggcacgcca gcacgacgtc tggcactacg
    49741 ccttatggag tttaatccag caaagtgaaa ttctgtttgc ccaagggttc ctgcaaaccg
    49801 cgtgggaaac acaggaaaaa gcgttccagc tgatcaacga gcaacatctg gaacagctgc
    49861 cgatgcatga gtttctggtg cgcatccgag cacaactgtt atgggcctgg gcacggctgg
    49921 atgaagccga agcgtcggcg cgtagcggga tggaagtctt gtcgtcttat cagccacagc
    49981 aacagcttca gtgcctggca atgttgattc aatgctcgct ggcccgtggt gatttagata
    50041 acgcccgtag ccagctgaac cgtctggaaa acctgctggg gaatggcaaa tatcacagcg
    50101 actggatctc taacgccaac aaagtccggg tgatttactg gcaaatgacc ggcgataaag
    50161 ccgccgctgc caactggttg cgtcatacgg ctaaaccgga gtttgcgaac aaccacttcc
    50221 tgcaaggtca atggcgcaac attgcccgcg cacaaatctt gctgggcgag tttgaacctg
    50281 cggaaattgt tctcgaagaa ctcaatgaaa atgcccgcag tctgcggttg atgagcgatc
    50341 tcaaccgtaa cctgttgctg cttaatcaac tgtactggca ggccggacgt aaaagtgacg
    50401 cccagcgcgt gttgctggac gcattaaaac tggcgaatcg caccggattt atcagccatt
    50461 ttgtcatcga aggcgaagcg atggcgcaac aactgcgcca gctgattcaa ctcaatacgc
    50521 tgccggaact ggaacagcat cgcgcacagc gtattctgcg agaaatcaat caacatcatc
    50581 ggcataaatt cgcccatttc gatgagaatt tcgttgaacg tctgctgaat catccggaag
    50641 tgccagaact gatccgcacc agcccgttga cccaacgtga atggcaggtc ctggggctga
    50701 tttactccgg ttacagcaat gagcaaatag cgggtgaact ggaagtggcg gcaaccacca
    50761 tcaagacgca tatccgcaat ctgtaccaaa aactcggcgt cgcccaccgt caggctgccg
    50821 tgcagcacgc gcagaaactg ctgaagatga tgggttatgg ggtgtgagtt tagccggata
    50881 atgcattgga tccggtttac atctttacat cattcaatac tcacccgcgt tacgccatct
    50941 gtttctatca aaccaaaccg caccggcaag aaacgctcca ccaccgcgat attggtcagc
    51001 agatggcatg aaggatgggc gaccttaaat tctcccgcgc ccgccagcgc catcggcagt
    51061 accagttggt cggcgagata ttcccccacc gccgctgggc ttgccaggta gcgtttcacc
    51121 tctttcacta actgcgcggc taccacctcg gcactgacgc gcttttcacc gacgacgaaa
    51181 aagcgttcag tgatattttc actttcgact tccagcgaga cggtattacc cggcccctgg
    51241 tcgcgcggca ggttatgaat attctgttca tgcagggaaa aactccccgc cagtgtagcg
    51301 atttcacgct cagcaacatg gcgcggcaca ccagctaata gaacttctcc acgcatctgc
    51361 acaatgttcc cgcgctcgcc aagttgcaag gtgttaaacg atgccacggg cgagacttcc
    51421 gttgccacca cgccgcctcc ggcaggataa aaaccgtgac gtaacagcgt ggtttgctga
    51481 tgaattccta ttttcgccag cagcggctcc agcacccggc ggataaaatc cgcaggcggg
    51541 gccgacgggt tatcggtgcc tccgctcact tcaacacgcg aaggtccatc ggcaaaccac
    51601 agcgcgggca gcaccgtttg cagcaccagc gtacagcttc cggcactacc gatagcaaag
    51661 cggtactcgc cgccgcgcac ggtgccaggc cggaagacca gacgctgcga ccccagctcc
    51721 gcgccttcca ccgttgccct acaaatttcc gcagccgctt ttaccgcggt cagatgctgg
    51781 cgcaacagcc ccggtttcgc ccgcccggca cgaatgctgg tgatggtaaa tggctggccg
    51841 gttatcatcg acaggctcag cgccgagcgc aggatctgcc cgccaccttc gccctgtgcg
    51901 ccatccagcg caatcatcct tttcatcatt tatcctttta cgcacaccac ctgacgcagg
    51961 gtatagataa cttccaccag atcgctttgt gccgccatca ccgcatcaat atctttatac
    52021 gccatcggga tttcgtcgat cacttcggca tctttacggc attccacatg cgcggtggca
    52081 cgaatttgat cttccacgct gaacagtttt ttcgctttag tacggctcat tacccgcccg
    52141 gcaccgtggc tgcacgaaca gaacgactct tcatttccca gcccacggac gataaagctt
    52201 tttgctccca tcgaaccggg aataattcca tattgaccag cacgcgcaga caccgcgcct
    52261 ttacgcgtca cgtagatctc ttcaccaaag tgctgttctt tttgcacata gttgtggtga
    52321 cagttgatct cttccatcgc cagcgtttgt ggctgtctga ccgttttctg cgtaatgctc
    52381 tgcaacgccg ttaccacgtt ttccatcatc gcatcgcggt taaggctggc gaaaagctgc
    52441 gcccaggcca cggctcttag gtaatcatca aagtattccg taccttccat aaagtacgcc
    52501 aggtcgcgcg atggcaacgt ttcaagctgg tcctgcatct ctttttgtgc caaatcgata
    52561 aagtaagtcc cgatggcgtt accaattccg cgtgaaccgg agtgcagcat aatccacacc
    52621 tggtctgact catcaaggca gatttcaata aagtggttac ccgttcccag cgttcccagg
    52681 tgtttatagt tgttagtgtt aaggaaacgc ggatattttt gcgttaacca ctgataaccg
    52741 gcttcaagct cagcccattt cgcatcgacg ttaacaggcg ggttttccca tgcgccttta
    52801 tcacgtttac aacggccagt ggtacgcccg tgcggcacgg ccatttcaat cgcctgacgc
    52861 agctctgcca ggttttcagg caggtcttcc gccgttaacg cggtacgcag cgcgttcatt
    52921 ccacagccaa tatccacgcc caccgccgcc ggaataatcg cccctttggt cgggatcacg
    52981 ctaccaatgg tggaaccttt acccaggtgt acatcaggca ttaccgcaat atgtttgaaa
    53041 ataaacggca tcttcgccgt attaataagt tgctgacgcg catcggcttc taccggcacg
    53101 cctttggtcc acatttttac cggggcattt tcagtggtca gtaattcgta attcattttg
    53161 tttttctctt ttcgttgttt gctgtcgtga taattgcaac cgtcgtgcca gaaaattaaa
    53221 taagcagatt aattttttaa ttcattgttt ttaaataggt taaatcttta cgtccgtaaa
    53281 cggagatttt ccacaaaacc aattcaccga taatgaaata tcgtctttta taaggatatc
    53341 taagatgcgt aaaacagtgg cttttggctt tgtcggtacc gtgctggatt atgccggacg
    53401 cggcagtcag cgctggtcga aatggcgtcc gacgctttgt ttatgccagc aagaatcgtt
    53461 agtcatcgat cggctggaat tgttgcacga cacccgctcg cgctcgctat tcgaaacgct
    53521 taaacgcgat atcgccagcg tttcgccaga aacagaagtc gtgggcgttg agattgaact
    53581 gcataacccg tgggatttcg aagaggttta cgcctgcctg catgatttcg cccgtggtta
    53641 cgagtttcag ccagaaaaag aagactattt aattcacatc accaccggca cccacgtcgc
    53701 gcagatttgc tggtttctgc tggcggaagc gcgttacctg cccgcccggc tgatacaatc
    53761 ttcacctccg cgcaaaaaag aacagccgcg cggcgcaggt gaagtgacga ttatcgatct
    53821 cgatttaagc cgttataacg ccatcgccag ccgctttgcc gaggaacgcc agcaaacgct
    53881 tgattttctt aagtccggca ttgccacgcg taacccccac ttcaaccgca tgattgagca
    53941 gatcgaaaaa gtggcgatca aatcccgcgc gccgattctg cttaacggtc caaccggcgc
    54001 gggcaagtca tttctggcgc gacgcatctt cgagttaaaa caggcgcggc atcagtttag
    54061 cggcgcgttt gtggaagtga actgcgccac cctgcgcggc gataccgcca tgtcgacgct
    54121 gtttggtcat gtaaaaggcg cgtttaccgg ggcgcgggaa tctcgtgaag gtttattacg
    54181 cagcgccaac ggcggaatgt tgtttcttga tgagattggc gaactgggcg cagacgaaca
    54241 agcaatgctg ctaaaagcca ttgaagagaa aaccttttac ccgtttggca gcgatcgcca
    54301 ggtgagcagt gattttcagc ttatcgccgg aacggtgcgc gatttgcgcc agctggttgc
    54361 cgaaggcaaa tttcgcgaag atctgtacgc gcggatcaat ctctggacct tcaccctgcc
    54421 gggtctacgc cagcgccagg aagatattga accgaacctt gattatgaag tggagcgcca
    54481 cgccacactc actggcgaca gcgtgcgttt taacaccgaa gcgcggcgcg cctggttggc
    54541 ttttgcgacc tctccccagg caacatggcg cggtaacttt cgcgaacttt ctgccagcgt
    54601 cacgcggatg gccacctttg ccactagcgg acgcatcact ctggacgtgg ttgaagatga
    54661 gataaaccgt ctgcgctata actggcagga gagccgcccc tccgcgctta cggcgttgct
    54721 gggcgcagag gcagaaaaca tcgacctttt tgacaggctg caactggaac acgtgatagc
    54781 tctctgtcgc caggcaaagt cgctttccgc tgccggacgc ctgctttttg atgtttcgcg
    54841 ccagggcaaa gccagcgtca atgacgcgga tcggctacgc aaatacctgg cgcgttttgg
    54901 tctgacgtgg gaagccgtgc aggatcagca cagctccagt tgaatatgat ggtccgtcag
    54961 cacctgcatt acgcttactg gcggcggggc gtcggtgtag acggcatcta ccatgctgat
    55021 gctgcccata ttgaccatcg cgttacggcc aaatttcgag tgatcgacaa ccagcataac
    55081 gtggcgcgag ttctcaataa tggcgcgctt ggtgcgaact tcgtggtaat cgaactccag
    55141 cagcgagccg tcgctatcga tgccgcttat ccccagaatg ccgaaatcaa ggcggaactg
    55201 ggagataaaa tcgagcgtcg cttcgccaat gatcccgcca tcgcggctgc gtaattcgcc
    55261 accggcgaga ataatgcgga aatcttcttt taccatcaac gtgttagcaa cattgagatt
    55321 gttggtgaca atacgcaaat tgctgtgatt aagcagcgcg tgcgccaccg cttccggcgt
    55381 ggtgccgata tcgataaaca gtgtcgagcc gttggggatt tgctccgcca ctttgcgggc
    55441 aatcctctct ttttcttcgg tctgggtggc tttgcgatcg tgccacggcg tgttaaccga
    55501 actggaaggc agcgccgcac cgccatggtg acgcaggatc aggttttgct ccgccagctc
    55561 attgaggtcg cggcgaatag tctgcgggct gacggagaaa tgctctacca gctcttcagt
    55621 actgacataa ccctgctgtt taaccagttc gataataccg ttgtgacgtt gtgtttgttt
    55681 catttataaa tccctggaat tattttcgtt ttcgcgcatt gagcgaatca acaaaagcca
    55741 tcgctaaacc cacggctaac ccggcgatgt gtgctccgtt cgccatcgac atcccaaaca
    55801 aatcaaacca tccggcgaca atccagatca gcgcaaagat aattaaccca cgttgcaggt
    55861 aaatgccact ttgcggatcg cgttcaccac gtagccagac gtagcccatc agcgcataca
    55921 ccacgccaga aagcccgcca aaccacggcc cgctgaattt ttgctgcaca tagccgctta
    55981 acagggcgct aatgagagta ataacaatta gcttaccgct accgaggcgt ttttccaccg
    56041 caccgccgag ataccaccac cagagcaggt taaagaggat atgcatcagc gagaagtgca
    56101 ttaacgcgtg ggtgaagtaa cgccagaact caaatttcag tgttggatcg aatggccagg
    56161 ccagccataa catcacttcc tgatcgccga gaatttgcat ggcgataaac accaccacgc
    56221 aggcgatcat caccacccag gttaccggac ctgcgcgttc acgcaaggcg gcaaagaaag
    56281 gataacggcg ataatgcagg ccactgccgg tatggcctgc ctgccagctg gccgccagat
    56341 aacgcggatc tgccgggttt tcgagaaaac gcgccagctc cgcccgtacg cgctcggcct
    56401 gggactcatc cgccagccag acatcgcttt ggttatgttg ttgaatcgtg aggataacac
    56461 cctgcgtcgc catgtaatca acaaacgcct gcgccacgcg ggggttagca aaagaggtaa
    56521 tcatcaacat cgttgctgtc gcttattcca cacaaaaggg gacagtataa agcgttacgc
    56581 gccgtacgcc acctctgcgg gaaactgacg ttgccaggct tcaaagccgc cgtcaatgct
    56641 atagaccaca tcgtagccct gttgcagcag atactgcgcc gcgcctttgc tgctattgcc
    56701 gtgataacac atcaccatca ccggagtgtc aaagtcgtta tcacgcataa acgcgcccag
    56761 cgtgtcgttg gttaaatgga aagcctgcac cgcatgcccc atagcgaaac tctgtggatc
    56821 gcgaatatcg accagcaccg cctctttttc ctgcaacttc tggtgcgcgt cggcaacgtt
    56881 aatacattcg aactgatcca tgcgtctctc tttctttaca aacaagtggg caaatttacc
    56941 gcacagttta cgtcgaagcg gcagataaac gccataatgt tatacatatc actctaaaat
    57001 gttttttcaa tgttacctaa agcgcgattc tttgctaata tgttcgataa cgaacattta
    57061 tgagctttaa cgaaagtgaa tgagggcagc atggaaacca aagatctgat tgtgataggt
    57121 ggcggcatca atggtgctgg tatcgcggca gacgccgctg gacgcggttt atccgtgctg
    57181 atgctggagg cgcaggatct cgcttgcgcg acctcttccg ccagttcaaa actcattcac
    57241 ggtggcctgc gctaccttga gcactatgaa ttccgcctgg tcagcgaggc gctggctgaa
    57301 cgtgaagtgc tgctgaaaat ggctccgcat atcgccttcc cgatgcgttt tcgcctgcca
    57361 catcgtccgc atctgcgccc ggcgtggatg attcggattg gtctgtttat gtacgatcat
    57421 ctgggtaaac gcaccagcct gccgggatca accggtttgc gttttggcgc aaactcagtg
    57481 ttaaaaccgg aaattaagcg cggattcgaa tattctgact gttgggtaga cgacgcccgt
    57541 ctggtactcg ccaacgccca gatggtggtg cgtaaaggcg gcgaagtgct tactcggact
    57601 cgcgccacct ctgctcgccg cgaaaacggc ctgtggattg tggaagcgga agatatcgat
    57661 accggcaaaa aatatagctg gcaagcgcgc ggcctggtta acgccaccgg cccgtgggtg
    57721 aaacagttct tcgacgaggg gatgcatctg ccttcgcctt atggcattcg cctgatcaaa
    57781 ggcagccata ttgtggtgcc gcgtgtgcat acccagaagc aggcctacat tctgcaaaac
    57841 gaagataaac gtattgtgtt cgtgatcccg tggatggacg aattttccat catcggcacc
    57901 accgacgtag agtataaagg cgatccgaaa gcggtgaaga ttgaagagag tgaaatcaac
    57961 tatctgctga aagtgtataa cgcgcacttt aaaaaacagc ttagccgtga cgatatcgtc
    58021 tggacctact ccggtgtgcg tccgctgtgt gatgatgagt ccgactcgcc gcaggctatt
    58081 acccgtgatt acacccttga tatccatgat gaaaacggca aagcaccgct gctgtcggta
    58141 ttcggcggta agttgaccac ctaccgcaaa ctggcggaac atgcgctgga aaaactgacg
    58201 ccgtattatc agggcattgg cccggcgtgg acgaaagaga gcgtgctgcc gggtggtgcc
    58261 attgaaggcg accgcgacga ttatgccgct cgcctgcgcc gccgctatcc gttcctgact
    58321 gaatcgctgg cgcgtcatta cgctcgcact tacggcagca acagcgagct gctgctcggc
    58381 aatgcgggaa cggtaagcga tctcggggaa gatttcggtc atgagttcta cgaagcggag
    58441 ctgaaatacc tggtggatca cgaatgggtc cgccgcgccg acgacgccct gtggcgtcgc
    58501 acaaaacaag gcatgtggct aaatgcggat caacaatctc gtgtgagtca gtggctggtg
    58561 gagtatacgc agcagaagtt gtcgctggcg tcgtaaggat gatgcccggt tgtgaagtgc
    58621 aaccgggcta tttggattcc ttagactatt cattgtaaat aattgaataa cggcccctga
    58681 gctaaaatcg ccaaacttaa gcacgttacc caatcgcccc acttcttcct ctggtttcta
    58741 ttttttctgc accaatggca ataatatccc aatgcaaaac caagctgtgc actaatcggg
    58801 ccgacaaaat cagtccaagg ctgcggccaa aaaggggaaa aattcagggg aagccagaca
    58861 aacgtcagaa agaaatttcg gatattttcc agcaacgctg atttctctgg agaaatataa
    58921 agaatgaaat tcgagagaat taaccagaaa tagaccggcg aaaagaccag ccagcaatgc
    58981 agtctgtttt ttaattcagg aggcgttctg cgggagaatt gccaaccgat gccagccatt
    59041 acactgaaaa aaagtgcata ttcaaccatt aactcaagtt gtagttcctg aagagaaata
    59101 cgcacaacca ggagcggaaa ggagaatatt atttctgcca gaaaagttaa cgtgaatata
    59161 agtacggcta agacaataac cgtcggccat accaataatg gcacgataac aaaaccgaga
    59221 attacagggg gaatataaag ccggaattta atcattttta ttttcctggt gattcggatg
    59281 atgcgtcata catcgcgcat aaacactcat caattatcca gtcgtctacc cagacctgat
    59341 gctgtgtgcc ctgttctggc tgctttggtg ggttctcaat cgcttgccgc gctctttctt
    59401 tcagtaaagg atgattattt ctagcccagg cattgatttg ggtggtaatg gcgttatcga
    59461 tatcgtcctg gttatctctg atataatcct cacaatatct gttcaataaa caggcaagag
    59521 aaacggtcga ccaaaagccg ggaactgaat taatggcatc cagcaatggc gcaagagcga
    59581 tatcggcacg gaaaaacatt tccagcatat actgtaattc gacagcttca tcgccggata
    59641 cccaatcagt catatactgg caggcaaatc gctgtaaaat tgcccgttca tccgcacgcc
    59701 agttttcttt gttggcaagg ttgatacggc gcagagaaaa aactacgtga atattgtcaa
    59761 aatgcccctg tgcaacaaat tccagtaaac gctgcagaaa atagcggacc tcatcgctat
    59821 tttgcggatc aggccctggg ctactattcc aggcattgat taaggaatac gggatcgctc
    59881 gtaacgatgt actccgtaat gccttttgtt cctgctgact caggcaatat tcacaacaaa
    59941 ccacaaattt ctgcggcacc cgataacgac gaaaggtgtg ataaatctgc tcaattaatg
    60001 cctgcatggc ggggctgggt tgcatagtta ccccctcgcc cgctgatcgc gtgtaaacgg
    60061 ttgatagaca aaacgagcat tcgccgcgtt caggcgaata agggcgctac gcgaaaggga
    60121 aaagctgggg cgaatgcaac tattgcagtc tcgacagctt tcctctgcgc aattttccac
    60181 tgagtaatga cgcgtgtaga gtttcagttc ggcctgctta tcgcgaaatc cgcgtaaact
    60241 gttattgtta tgaaatttcc aagcatcttt aaagacctga aagatataga catcctcaat
    60301 ccaggagtca cattttttaa gcgggcgctt tgctattatc acccgctcat ccggatttag
    60361 catcgccgta aattgtacgg tgacgtcatt tgtcgtgttc aggtcacatt gaacatctgg
    60421 tacatcactg atataggggt caaatccaaa atcagcacca caggcacaaa gacggctaat
    60481 ggtggcggta tcgagggaaa atcccggcca taccttgctg gaggttaagc caatatttaa
    60541 tgtgacatca tatcgaagca atccctcaag accgtcagca ccgacgaact tttccccttc
    60601 atccagggcc tggttaataa gttgctgaac aatgccttca tcccagtatg ccgatttgca
    60661 gttggcaatc gtaatatcca cgtctgtgaa gggatcagta ccggcaaggc ttgcaaataa
    60721 tgtattaact aattccattt ttattatcca gctttaaggt agcgtcttat acatcgggac
    60781 ataacctaca tcttccacca aactctgtcc ctgcggcgtt aaaaaccact ccaacaattt
    60841 ttgtgtctct gacgtcgtat tttcccgcgt taccataaag gcatcgacga tatagggata
    60901 tttgccgttg cgaatgtttt cggcagtcgg tgcaataccg ttaatcgcta gcagttttat
    60961 atttttatcg tcgttcattt gtgttgcgta ataacggaag gtgtaaccta ttgcgttgtt
    61021 tgtattacgg tattcggcaa cgactttaat cattccctcc atcatacttg ccacttctgt
    61081 ttcctgcggc gagatcatgc ggacattttt catgacctgg gattgcatca ccgtctggct
    61141 accagagtct tccgggcgtt gccaggtctg gatctcctga tcgttaccgc caaccgtgcg
    61201 ccagttggta attgcaccac tgaagatgtc acgcacttgt tgttcggtca gggaattaac
    61261 cgggttatct acattgacga tgaaaacaaa cgcttcacgg gcaaaaggcg tgtaaatcaa
    61321 agtgacgccc gattcctccg cgcgtttttt ctgcccaccg gaaggttgag ccacaaagat
    61381 aatatcggca ttacctttaa caatcttgtt atatgcttcg ggagtacgag agttcgccag
    61441 atattcccat tcgtgaaaat cttccggcaa aacgcttagt gcataaaagg cagaggcata
    61501 aatggggtac gccgccgttg cgccatcaag tcgcggccag ttttgcgtga actgaatttg
    61561 cggtttacca cgcagtggtg tcagttgatt attgagttta tcgggtcgcc aggcccaggt
    61621 atcaatattt tcacgcacac cgacgccggg attaagctgt ttatataaat aagcctggta
    61681 accggcgaca aaaccaagca tgagaaggat aaacaagaga gaattacggc aacgtaaccc
    61741 ggacgtgccc gttaaaggat atctggtcgg ccagccgtac cccagcgaaa aactgatatg
    61801 cgcgccaaca ggtataatga ctaaccacca gtactcacca ccgactaatg aggctaacaa
    61861 aacgcccagc caggggagca taagatcagc caaccaaccc gctcccatct ggcgaaattg
    61921 ataatctgcc agatgcagcg cgattatcca gacccccagg gttaataatg cgggtactaa
    61981 aaaaagacat tgtcgccaga cggctatttc tcttttttct ggtgcactat cgtccgccca
    62041 cactcgcccc gagaaaaata gcattaatgc gcccagcgcc agcataaaaa tgttgagcag
    62101 aaggtaaatt gttggcgtga taaagatatt acaaacttca ataataccgg caagtccgac
    62161 acccagcatg gcaataaccg ccgccaaaaa ctgcgccaga atggggatgc cgaaaaaagt
    62221 cattaccagc gaggtcaaaa tccattttct gttttgcatt attctttcca ttctttctga
    62281 atggcgaaat tatactcccc gagtcccctt gccccttctg gacacttttc cgaaatgatg
    62341 gcgaaaaaaa cgggaccctt tgg
//
LOCUS       DAZGVQ010000006        60090 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000006 DAZGVQ010000000
VERSION     DAZGVQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.006"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1206)
                     /gene="xapB"
                     /locus_tag="ACHLAG_000351"
     CDS             complement(<1..1206)
                     /gene="xapB"
                     /locus_tag="ACHLAG_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFP0653660.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKAVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGF"
     gene            complement(1266..2099)
                     /gene="xapA"
                     /locus_tag="ACHLAG_000352"
     CDS             complement(1266..2099)
                     /gene="xapA"
                     /locus_tag="ACHLAG_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFP0653661.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            2348..3112
                     /gene="yfeN"
                     /locus_tag="ACHLAG_000353"
     CDS             2348..3112
                     /gene="yfeN"
                     /locus_tag="ACHLAG_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFP0653662.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAQFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(3151..4077)
                     /gene="yfeR"
                     /locus_tag="ACHLAG_000354"
     CDS             complement(3151..4077)
                     /gene="yfeR"
                     /locus_tag="ACHLAG_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0653663.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            4167..5165
                     /gene="yfeH"
                     /locus_tag="ACHLAG_000355"
     CDS             4167..5165
                     /gene="yfeH"
                     /locus_tag="ACHLAG_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFP0653664.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSTDKA"
     gene            complement(5162..5380)
                     /gene="ypeB"
                     /locus_tag="ACHLAG_000356"
     CDS             complement(5162..5380)
                     /gene="ypeB"
                     /locus_tag="ACHLAG_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFP0653665.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(5382..7397)
                     /gene="ligA"
                     /locus_tag="ACHLAG_000357"
     CDS             complement(5382..7397)
                     /gene="ligA"
                     /locus_tag="ACHLAG_000357"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFP0653666.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(7468..8454)
                     /gene="zipA"
                     /locus_tag="ACHLAG_000358"
     CDS             complement(7468..8454)
                     /gene="zipA"
                     /locus_tag="ACHLAG_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFP0653667.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVTDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            8684..9445
                     /gene="cysZ"
                     /locus_tag="ACHLAG_000359"
     CDS             8684..9445
                     /gene="cysZ"
                     /locus_tag="ACHLAG_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFP0653668.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            9630..10601
                     /gene="cysK"
                     /locus_tag="ACHLAG_000360"
     CDS             9630..10601
                     /gene="cysK"
                     /locus_tag="ACHLAG_000360"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFP0653669.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            10985..11242
                     /gene="ptsH"
                     /locus_tag="ACHLAG_000361"
     CDS             10985..11242
                     /gene="ptsH"
                     /locus_tag="ACHLAG_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFP0653670.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            11287..13014
                     /gene="ptsI"
                     /locus_tag="ACHLAG_000362"
     CDS             11287..13014
                     /gene="ptsI"
                     /locus_tag="ACHLAG_000362"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFP0653671.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            13055..13564
                     /gene="crr"
                     /locus_tag="ACHLAG_000363"
     CDS             13055..13564
                     /gene="crr"
                     /locus_tag="ACHLAG_000363"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP0653672.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(13606..14457)
                     /gene="pdxK"
                     /locus_tag="ACHLAG_000364"
                     /gene_synonym="thiJ"
     CDS             complement(13606..14457)
                     /gene="pdxK"
                     /locus_tag="ACHLAG_000364"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFP0653673.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            14562..14930
                     /gene="yfeK"
                     /locus_tag="ACHLAG_000365"
     CDS             14562..14930
                     /gene="yfeK"
                     /locus_tag="ACHLAG_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFP0653674.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(14933..15844)
                     /gene="cysM"
                     /locus_tag="ACHLAG_000366"
     CDS             complement(14933..15844)
                     /gene="cysM"
                     /locus_tag="ACHLAG_000366"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFP0653675.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(15978..17075)
                     /gene="cysA"
                     /locus_tag="ACHLAG_000367"
     CDS             complement(15978..17075)
                     /gene="cysA"
                     /locus_tag="ACHLAG_000367"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFP0653676.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(17065..17940)
                     /gene="cysW"
                     /locus_tag="ACHLAG_000368"
     CDS             complement(17065..17940)
                     /gene="cysW"
                     /locus_tag="ACHLAG_000368"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFP0653677.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(17940..18773)
                     /gene="cysT"
                     /locus_tag="ACHLAG_000369"
     CDS             complement(17940..18773)
                     /gene="cysT"
                     /locus_tag="ACHLAG_000369"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFP0653678.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(18773..19789)
                     /gene="cysP"
                     /locus_tag="ACHLAG_000370"
     CDS             complement(18773..19789)
                     /gene="cysP"
                     /locus_tag="ACHLAG_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFP0653679.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKNKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(19960..20751)
                     /gene="ucpA"
                     /locus_tag="ACHLAG_000371"
     CDS             complement(19960..20751)
                     /gene="ucpA"
                     /locus_tag="ACHLAG_000371"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFP0653680.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVCTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(20880..21737)
                     /gene="murR"
                     /locus_tag="ACHLAG_000372"
     CDS             complement(20880..21737)
                     /gene="murR"
                     /locus_tag="ACHLAG_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFP0653681.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            21901..22797
                     /gene="murQ"
                     /locus_tag="ACHLAG_000373"
     CDS             21901..22797
                     /gene="murQ"
                     /locus_tag="ACHLAG_000373"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP0653682.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            22801..24225
                     /gene="murP"
                     /locus_tag="ACHLAG_000374"
     CDS             22801..24225
                     /gene="murP"
                     /locus_tag="ACHLAG_000374"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFP0653683.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNN
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            24230..25534
                     /gene="pbp4b"
                     /locus_tag="ACHLAG_000375"
     CDS             24230..25534
                     /gene="pbp4b"
                     /locus_tag="ACHLAG_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFP0653684.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(25592..26491)
                     /gene="yfeX"
                     /locus_tag="ACHLAG_000376"
     CDS             complement(25592..26491)
                     /gene="yfeX"
                     /locus_tag="ACHLAG_000376"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFP0653685.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(26587..27162)
                     /gene="yfeY"
                     /locus_tag="ACHLAG_000377"
     CDS             complement(26587..27162)
                     /gene="yfeY"
                     /locus_tag="ACHLAG_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFP0653686.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(27223..27672)
                     /gene="yfeZ"
                     /locus_tag="ACHLAG_000378"
     CDS             complement(27223..27672)
                     /gene="yfeZ"
                     /locus_tag="ACHLAG_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFP0653687.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(27659..28084)
                     /gene="ypeA"
                     /locus_tag="ACHLAG_000379"
     CDS             complement(27659..28084)
                     /gene="ypeA"
                     /locus_tag="ACHLAG_000379"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFP0653688.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            28298..29167
                     /gene="amiA"
                     /locus_tag="ACHLAG_000380"
     CDS             28298..29167
                     /gene="amiA"
                     /locus_tag="ACHLAG_000380"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFP0653689.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            29171..30070
                     /gene="hemF"
                     /locus_tag="ACHLAG_000381"
     CDS             29171..30070
                     /gene="hemF"
                     /locus_tag="ACHLAG_000381"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFP0653690.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(30076..31128)
                     /gene="eutR"
                     /locus_tag="ACHLAG_000382"
     CDS             complement(30076..31128)
                     /gene="eutR"
                     /locus_tag="ACHLAG_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFP0653691.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(31174..31674)
                     /gene="eutK"
                     /locus_tag="ACHLAG_000383"
     CDS             complement(31174..31674)
                     /gene="eutK"
                     /locus_tag="ACHLAG_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFP0653692.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLCKRSSR
                     YRLKPH"
     gene            complement(31687..32346)
                     /gene="eutL"
                     /locus_tag="ACHLAG_000384"
     CDS             complement(31687..32346)
                     /gene="eutL"
                     /locus_tag="ACHLAG_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFP0653693.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(32356..33243)
                     /gene="eutC"
                     /locus_tag="ACHLAG_000385"
     CDS             complement(32356..33243)
                     /gene="eutC"
                     /locus_tag="ACHLAG_000385"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFP0653694.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(33264..34625)
                     /gene="eutB"
                     /locus_tag="ACHLAG_000386"
     CDS             complement(33264..34625)
                     /gene="eutB"
                     /locus_tag="ACHLAG_000386"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFP0653695.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(34637..36040)
                     /gene="eutA"
                     /locus_tag="ACHLAG_000387"
     CDS             complement(34637..36040)
                     /gene="eutA"
                     /locus_tag="ACHLAG_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFP0653696.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(36037..37263)
                     /gene="eutH"
                     /locus_tag="ACHLAG_000388"
     CDS             complement(36037..37263)
                     /gene="eutH"
                     /locus_tag="ACHLAG_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFP0653697.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            complement(37380..38567)
                     /gene="eutG"
                     /locus_tag="ACHLAG_000389"
     CDS             complement(37380..38567)
                     /gene="eutG"
                     /locus_tag="ACHLAG_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFP0653698.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDTVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(38557..39393)
                     /gene="eutJ"
                     /locus_tag="ACHLAG_000390"
     CDS             complement(38557..39393)
                     /gene="eutJ"
                     /locus_tag="ACHLAG_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFP0653699.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(39404..40807)
                     /gene="eutE"
                     /locus_tag="ACHLAG_000391"
     CDS             complement(39404..40807)
                     /gene="eutE"
                     /locus_tag="ACHLAG_000391"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFP0653700.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(40819..41106)
                     /gene="eutN"
                     /locus_tag="ACHLAG_000392"
     CDS             complement(40819..41106)
                     /gene="eutN"
                     /locus_tag="ACHLAG_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFP0653701.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(41213..41506)
                     /gene="eutM"
                     /locus_tag="ACHLAG_000393"
     CDS             complement(41213..41506)
                     /gene="eutM"
                     /locus_tag="ACHLAG_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFP0653702.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(41545..42561)
                     /gene="pta"
                     /locus_tag="ACHLAG_000394"
     CDS             complement(41545..42561)
                     /gene="pta"
                     /locus_tag="ACHLAG_000394"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP0653703.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(42558..43361)
                     /gene="eutT"
                     /locus_tag="ACHLAG_000395"
     CDS             complement(42558..43361)
                     /gene="eutT"
                     /locus_tag="ACHLAG_000395"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFP0653704.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(43358..44059)
                     /gene="eutQ"
                     /locus_tag="ACHLAG_000396"
     CDS             complement(43358..44059)
                     /gene="eutQ"
                     /locus_tag="ACHLAG_000396"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFP0653705.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(44034..44513)
                     /gene="eutP"
                     /locus_tag="ACHLAG_000397"
     CDS             complement(44034..44513)
                     /gene="eutP"
                     /locus_tag="ACHLAG_000397"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFP0653706.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(44526..44861)
                     /gene="eutS"
                     /locus_tag="ACHLAG_000398"
     CDS             complement(44526..44861)
                     /gene="eutS"
                     /locus_tag="ACHLAG_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFP0653707.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(45154..47433)
                     /gene="maeB"
                     /locus_tag="ACHLAG_000399"
     CDS             complement(45154..47433)
                     /gene="maeB"
                     /locus_tag="ACHLAG_000399"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFP0653708.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            47722..48672
                     /gene="tal"
                     /locus_tag="ACHLAG_000400"
     CDS             47722..48672
                     /gene="tal"
                     /locus_tag="ACHLAG_000400"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP0653709.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            48692..50695
                     /gene="tkt"
                     /locus_tag="ACHLAG_000401"
     CDS             48692..50695
                     /gene="tkt"
                     /locus_tag="ACHLAG_000401"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP0653710.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(50791..51834)
                     /gene="ypfG"
                     /locus_tag="ACHLAG_000402"
     CDS             complement(50791..51834)
                     /gene="ypfG"
                     /locus_tag="ACHLAG_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFP0653711.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(51960..52535)
                     /gene="nudK"
                     /locus_tag="ACHLAG_000403"
     CDS             complement(51960..52535)
                     /gene="nudK"
                     /locus_tag="ACHLAG_000403"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFP0653712.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(52603..54582)
                     /gene="aegA"
                     /locus_tag="ACHLAG_000404"
     CDS             complement(52603..54582)
                     /gene="aegA"
                     /locus_tag="ACHLAG_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFP0653713.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVGVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIINWLGVKSVKSH"
     gene            54788..56488
                     /gene="narQ"
                     /locus_tag="ACHLAG_000405"
     CDS             54788..56488
                     /gene="narQ"
                     /locus_tag="ACHLAG_000405"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFP0653714.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            56652..59765
                     /gene="acrD"
                     /locus_tag="ACHLAG_000406"
                     /pseudo
     CDS             56652..59765
                     /gene="acrD"
                     /locus_tag="ACHLAG_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
     gene            complement(59864..59923)
                     /gene="ypfM"
                     /locus_tag="ACHLAG_000407"
     CDS             complement(59864..59923)
                     /gene="ypfM"
                     /locus_tag="ACHLAG_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFP0653715.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        14038 a        16587 c        15361 g        14104 t
ORIGIN      
        1 aaacccaaag aaaaatatca ctgcgagaaa aagagcatat cctgcaaaca ccagccagat
       61 agtttgccag tcttttacgc catccaccga aaagtaatct actgccatgc cactcagaat
      121 cgagccaacc catgcgccga caccatttac catggtcata aagagcccct gcgcgctggc
      181 acgaatgctg gaatcaactt cctgttcgac aaataccgaa ccagaaatat tgaagaaatc
      241 gaatgcacag ccataaacaa tcatcgacag cagcagcaaa ataaatccgg ttgttgacgg
      301 atcgccatag gcgaagaagc caaagcgcag cgtccaggcc accatactca tcagcatgac
      361 ggctttaatg ccaaatcgct ttaaaaagaa tgggatagtc agtataaagc ccacttctgc
      421 catctgtgaa actgacagta aaatggaggg atatttcacc acaaaactgt cagcaaactc
      481 cgggttacgg gcgaaatcat gtaggaacgg attaccaaaa acgttggtaa tttgcagtac
      541 cgcacccagc atcatggcaa agaggaaaaa gatggccatg cgtggatttt taaacagcac
      601 gaaggcatcc agacccagct tgctggcaag cgatgtggtc gcttttttct ccgcaaccgg
      661 aatcttcggc aaagtcagcg cataagccga cagcagcaat gacgcaccgg acgcgatata
      721 cagctgcaga ctactcaatt ccagatgcag caggcttact gcccacatcg cgacaatgaa
      781 ccccaccgta ccaaaaacgc gaatgggcgg gaaagcggtc accgggtcaa gccctgcctg
      841 ggcaagacag gaataagaga cgctgttcga taacgcaata gtcggcataa acgccatcgc
      901 attgactaac atcacccaaa acatcatatc cggatcagtt acggatgccg cataaaaaag
      961 tacgcccgca cacaccaggt gacacagcat gtatgcacgt tctgcgcgca gccatttgtc
     1021 tgcgatgatc cccattatac caggcataat aatcgcggcg atccctttgg aactgtaaac
     1081 catgccaaca ttagcgccgg tgaaatgaag ggtattaatc atgtaagagc cgagggtaac
     1141 cagccagctc ccccagataa aatattgcaa aaaggacatt acctttaagc gcatcgcgat
     1201 gctcataatc gtttccttgt cgcatcattt ggcctcaccc gcgaggccgt ttcttattgt
     1261 ttttctcagg caattttgcg cagaaagcca caaataaggt tgatgaagtt ctgcttagag
     1321 agctccgcag ccgccagcgt ttgcgcatgt gacaatttca catcgcttaa accttccgcc
     1381 atattggtaa tcgcagagac cgcaacgact ttaaggtcgc aatggcgagc tgaaataacc
     1441 tcaggcacca cagacatacc aacaacatcc ccaccaataa tttgcatcat gcgaatttcc
     1501 gccgcagtct cgaaattcgg ccccggatac gagacgaaca cgccctccgt cagagggaaa
     1561 ccctcttctt tcgccacttt ttgtaacagt gcgcggtatt ccgcatcgta ggcatttgcc
     1621 agtgagaaga agcgctctcc aaaacgatca tcgttaagac ccaccatcgg cgtaccgggc
     1681 atggtgttga tatgatcttt caatgcgacc aggctgcctg cccccacttc cggacgcagc
     1741 gagcctgccg cattggtgca gaacagtagc tcgcagccca gcagcttaaa ggtacgaatt
     1801 gcgtcagtca taatggtcat tccacgacct tcgtagaaat gtccgcgacc tttcatacat
     1861 accaccggta cgccctggag atggcccagc actaactccc ccgcatgacc atgtaccgta
     1921 cttaccggga agcccggcag cttttcgtag gaaattgcga cagcgttctc aatctgatcg
     1981 gccagcgcgc cgagcccgga acctaaaata aaggccactc gtggcgtgaa atcaggttta
     2041 taagtcttga taatatctat gcaaaacagc gggttatgag aaaattgaac ctgagacata
     2101 catatccttt ttctgtaggg tggaatctaa cgccttttta tagcgaggtt gtcccggctc
     2161 ttaccaatac acttttctca cgcattcaat agcgattcga tatcgatgga tttatatctt
     2221 ttctctatta atccacggta aaaactgtat tggcgatatt aattcctttg cattaacaat
     2281 atgtcgtttt tagcaaacca ctttattaag caatctacta ataacgatcg gcatagggaa
     2341 ttatgctatg aaaaaacatc ttttaactct gacactttcc tctatattag cgatacctgt
     2401 tgtatctcat gcacaattta aaggcggttt tgcagatatc ggcgtgcatt atctggactg
     2461 gaccagccgg actaccgaaa aatcgtcgac caaatcacac aaagatgatt ttggttatct
     2521 tgaatttgaa ggtggcgcta actttagttg gggagaaatg tacgggttct tcgactggga
     2581 aaacttctat aacggtcgcc ataataaacc aggtagcgag cagcgttata cctttaaaaa
     2641 taccaaccgt atttacctgg gtgacaccgg atttaatctc tacttgcatg cgtatggcac
     2701 ctacggttct gcgaatcgcg tgaatttcca cgacgatatg ttcctgtacg gtatcggcta
     2761 caatttcacc ggcagcggtt ggtggttcaa accgttcttt gctaagcgtt atacagatca
     2821 aacctattac actggcgaca acggctatgt cgccggctgg gttgcaggtt acaactttat
     2881 gctgggcagc gagaaattca ctcttaccaa ctggaacgag tacgagtttg accgtgacgc
     2941 tacctatgca gcgggtaatg gcggtaaaga agggctgaat ggcgcagttg cactgtggtg
     3001 gaatgcaaca tcacacatta ctacggggat tcagtatcga tatgcggatg acaaactggg
     3061 cgaagatttt taccaggatg cgataatcta ctccatcaaa tttaacttct gattgattac
     3121 tgccggggct cccggcagta tgaattcact ctatatctga tacaacggat ccccttcccg
     3181 accagccatt agcgcattgc cctggtctcg taccacgtcc cataacgctt cagcggcggt
     3241 ggaaagtgaa cggttcttcc tgcgcaccag catcaactgg cgttcaacaa ccggcgtaat
     3301 gcgctttacc accagtgggc taccttcagg caatggtagc gccagtgcgg gaagaatact
     3361 gatgccgatg cctgcggcta ccatcgggaa cagcgtcgcc ggatgtccaa tctcctgcac
     3421 aatattcgcc tgaataccat tgcgcgccag cgctgcgtca atcagcggtc ggctgcctga
     3481 cgcgtaatcc tgcaacacca atttcgctcc ctgtaatgcc tgccagggta cataatcttc
     3541 gacggccaaa gcgctatcgc gatggcaaag cagaaagaaa ggctcggaaa gaatcgcttc
     3601 acattgcaga tcgcccactg ggcctggatc gatgacgatg ccaaagtcga catctccctg
     3661 gcgaatactt tccatcaccc actgctgcgg acgatcgtgc aggacaaact gaatatctgg
     3721 atagcggcga tggctttcag caatgcattg cggaataaga tgcgcggaaa tggtctggct
     3781 ggcagcgacc cgaacttttc cgctcagttg ttgccccata cgcccggtat cgcgcaacgt
     3841 gctgttcagt tcatccagca gtcgctcaag acgcaaagcc agctgctgcc ctgcatctgt
     3901 aagcaccact tcacgcgtgg ttctgtccag caggcgaaca ccagtatgat tttccagttc
     3961 cttcacactg tgactcactg ccgactggct caggccgata cgctctcctg cacgactaaa
     4021 acttttctcc tgcgctactg tgacgaaaac ctttaattgt tttaaagaat aattcatctg
     4081 ttttcttcat gaatggatgc aataaatcaa ttttatttct caaactggaa gaagcacaat
     4141 agaaccatcg atcatctgga gtctttatga aactttttcg tatcctcgat cctttcacct
     4201 taaccctgat cacggtggtg ttgctggcct ctttctttcc ggccagaggc gatttcgtcc
     4261 ccttctttga aaatctgacc accgcagcga ttgccctgct gttctttatg cacggcgcga
     4321 agttgtcgcg tgaggcgatt attgctggcg gtggtcactg gcgactgcat ttgtgggtaa
     4381 tgtgcagcac cttcgtgctg tttccgattc tgggtgtact gtttgcctgg tggaaaccgg
     4441 taaatgtcga cccgatgctc tactccggtt ttctctactt gtgcattctc ccggctaccg
     4501 tgcagtctgc aatcgccttc acgtcaatgg cgggcggtaa cgtcgcggcg gcggtttgtt
     4561 ctgcgtcagc atccagcctg ctggggattt tcctttcacc attgctggtt ggtctggtga
     4621 tgaatgttca cggtgcaggg ggcagccttg agcaggtcgg taaaattatg ctgcaactgc
     4681 tgctgccgtt tgtgttgggg catctttccc ggccgtggat tggtgactgg gtgtcgcgca
     4741 ataaaaaatg gattgcgaaa actgaccaga cgtccattct gttggtggtt tatacagcgt
     4801 tcagcgaagc cgtcgttaat ggtatctggc ataaagttgg ctggggatca ttgctgttta
     4861 tcgtggtggt cagctgcgtt cttctggcta tcgtgattgt agttaacgtc tttatggcac
     4921 gccgactggg cttcaataag gcagatgaaa ttactatcgt cttttgtggt tcgaaaaaga
     4981 gtctggcaaa tggcatcccg atggcaaaca ttctgttccc cacatcggtg atcggtatga
     5041 tggtgctgcc cctgatgatt ttccatcaga tccaattgat ggtctgtgcg gtgctggcgc
     5101 gtcgatacaa acgccagacc gaacagttac aggcgcagca ggaaagcagc accgataaag
     5161 cttaaagcgg acgcttcagg ggctggacca gttgcgtcag cccctcggtt ttaatcagca
     5221 gcgtgatttg cattagctca ccgagcttcc ctgccgggaa ctcatctttg cgggcaaacc
     5281 acagcagata ctcctccggc aggtcgatta agcgacgccc tttgtatttg ccaaacggca
     5341 ttatcgtatt ggctatttca atcagctgct ctttttccat ctcagctacc cagcaaacgc
     5401 agcatttccg cttcgtcgat gacttcaatg cccagttcct gcgccttcgc cagtttagat
     5461 cctgcagctt cacccgctat caccagatcg gttttcttcg acacgctgcc cgcgactttc
     5521 gcgcccagtt cgaccagtcg agctttagcg tcatcacgtg acatctgact taagctgccc
     5581 gtaagcacca cggttttacc agcaaacggg ctgtcaatct cttccgcgtt gataacgatc
     5641 ggcgcaggcc agtggacacc ttccgccaac agctcgctga taacattgcg gttgctttct
     5701 tcggcaaaga agttgtgaac gtgggatgca acgacaatgc caacatcagg caccttttgc
     5761 agctcttcaa tcgaagcggc ttccagcgct tccagcgtgc cgaaatatgc cgccagacct
     5821 gctgcggtgg cctcgccgac ttcacggatg ccaagtgcat agaggaagcg agcaaaggtg
     5881 gtttctttcg ctttttctag cgcgttaacc acgttttgtg ccgattttgg ccccatacgc
     5941 tccagtccgg tcagtttgcc tgcggtgagt ttgaacagat ctgccggagt atggacatat
     6001 tctttttcaa ccagctgatc gatgattttg tcgcccattc cgtcaacatc catcgcacgg
     6061 cgggaaacaa agtgtttcag cgactcttta cgctgcgcac cgcaaatcag gccaccggta
     6121 cagcgggcaa ccgcttcacc ttccacacgc tcaacgtcag aaccacatac cggacaatgc
     6181 gttgggaata caacctcacg ggtatcttcc gggcgttcag aaagcacgac gttaaccacc
     6241 tgcgggatca cgtcgccagc gcggcgaatc accactttat cgccaatgcg taaaccaaga
     6301 cgttcgattt catccgcatt gtgtaaggtt gcgttactca ccagcacgcc agcaacatgg
     6361 acaggttcca gacgcgcaac aggcgtaatc gcgccagtac gcccaacctg aaactcgacg
     6421 tcacgcacaa aggtcatctg ctcctgcgcc gggaatttaa acgctaccgc ccagcgcggg
     6481 gcacgcgcga caaagccaag ctgctcctgc tgttccagtg agttgacctt aatcaccacg
     6541 ccgtcgatat caaagcccag cgtcgggcgg tcttcttcca ctttgtgata gaacgccagc
     6601 acttcttccg ccgattcaca aagcgttacc cgatcgctga ccggcaaccc ccacttttta
     6661 aattgcagta aacggccaag atgagtatcc ggcagctcgc caccttccag aacaccaacg
     6721 ccatagcaga aaaaagtgag cggtcgcttc gctgtaatac gcggatcaag ctgacgcagt
     6781 gaaccagctg ccgcattacg tgggttagca aaaactttcc cgcccgtgcg tcgcgcatct
     6841 tcgttaatct tttcgaaccc cgcctgcggc aggaacactt caccacgcac ttccagacgc
     6901 gccgggatat tctctccgtg cagcttcagc ggaatagcgc gaatagtacg cacattagac
     6961 gtgatatctt ccccggtggt gccatcgcca cgggtcgcgg cactgactaa aacgccattt
     7021 tcatacagaa tactgacggc aaggccatcc agcttcagct cacagcacca ggtgactttc
     7081 tcgttgtttt tcagacggtc ctgcacacgt ttgttgaaag caagaaagct ttcttcatca
     7141 aaaacgttat ccagtgacag cattggtact tcatggcgta tctggctgaa agccgccagc
     7201 ggcgcagcgc ctacacgttg ggtaggcgaa tcaggcgtaa tcagttctgg atgtttggtt
     7261 tccagctcgc gcagttcgcg catcagcctg tcgtattcag cgtcgggaat ttccggcgca
     7321 tccatcacat gataaagata ttcatgatgg cgaagcgtcg ttcgcagttc tgtcagttgt
     7381 tgttcgattg attccatatc gcaccatcaa tgctaaaaac ccccgacaag cgggggttcg
     7441 aagaggagtt aatttgcctt aggtgtatca ggcgttggcg tctttgactt cgcggatgat
     7501 gtcctggtac tcgcgcaatt tctgcggagt catcatacgg cgctgatcgt caagcacgac
     7561 accgcccact tcatcggcaa tatgctgcgc agattgcagc atcagcttga agttctgcag
     7621 ctcgtcaccg taagacggta cctgcataaa gatggtgaca cccggagtag tgaaatcctt
     7681 catttcagga tcaaaggttc ccggtttcac catattcgcc aggctgaata acgccgggcc
     7741 gctgccatcc gggctaagat gacgatggta aatattcata tcgccaaaaa tgaagcccgc
     7801 ttgttgaatg ctgttaagaa gcagttcacc gtttagctcg ctaccgtgat gcgccgcgac
     7861 gttcatgata atcaccgctt ctttgcgctt cggtttatcc gtaactggag ccggttccgc
     7921 tacaggctca ggctgtggtg ccgctacggg ttctgcaggc tggaaagcct gttgtgccgg
     7981 ttgcggtgct gaatgcacag gctgcggcgc tggcgcgacc tgtggcgaaa ctggctgctg
     8041 caacggctgt tcaggctgcg gctgataggc aggctgctgc accggctgcg ctggacgcgg
     8101 agcatgttgc ggcggtacct gcgcttcagg cggctgctgg accggttgac gcggctgcgc
     8161 agacgcataa ggcggttggt actggtgttg cggcgacgga cgagcagcct catgctcctg
     8221 agcgttagcc ggggcatgat tcacgcggtg aacacgaacc tcaccaacgc cctcatcatc
     8281 ttcgacatcc tcgtcataag aatcgtcgtc acgttttgac ttcattcgtt ttaatggccg
     8341 atcgcggaac atagaagatc gttctttacg gctggtccag aaaccatgta ccagtaaagc
     8401 gattatggcg atcgcgccaa caatgattaa tatcagacgc aaatcctgca tcattatatt
     8461 ctctgttgtt ctaacacctt gccaccacgg caaacattta ctcactaaga gtatttgccg
     8521 attacctcaa gtgcaagtgc actattaact ttcacagcac aaagatagat gaaatcgtgc
     8581 tttttgctgt tttttcgaac atatcctaac tgtccattgc gcaattaccc ggtaaaatac
     8641 gcagaatttt cctgggattg gtcaaaagga gctcatcctg attatggttt catcattcac
     8701 atctgcccca cgcagcggtt tttactattt tgcgcaaggc tggaagctcg tctcgcaacc
     8761 tgggattcgg cgtttcgtta ttttaccgct gctggtcaat attttgttga tggggggcgc
     8821 attctggtgg ctctttacac agctcgatgt ctggatcccg actctcatga gttacgttcc
     8881 ggactggcta caatggctga gttatctgtt gtggcctctg gctgtcatct ctgtgctgtt
     8941 agtgtttggc tatttcttct ccacgattgc taactggatt gccgctccgt ttaacggttt
     9001 attggctgaa caactggaag cacgattgac tggcgctaca ccgccagata ccgggatttt
     9061 cggtatcatg aaagatgtgc cgcgaatcat gaaacgcgaa tggcaaaaat ttgcctggta
     9121 tctgccgcgc gcaattgtat tgctaattct ttacttaatc cctggtattg ggcaaaccgt
     9181 cgcgccggta ctgtggttcc tgtttagcgc ctggatgtta gccatccagt attgcgatta
     9241 ccccttcgat aaccacaaag tgccgtttaa agagatgcgc accgccctgc gcacacgcaa
     9301 aatcaccaat atgcagtttg gtgctttaac cagcctgttt acgatgatcc cgctgcttaa
     9361 tctgttcatc atgcccgttg ccgtttgtgg cgcgacggcg atgtgggtcg attgctatcg
     9421 cgataaacac gcgatgtggc ggtaacaatc taccggttat tttgtaaacc gtttgtgtga
     9481 aacaggggtg gcttatgccg ccccttattc catcttgcat gtcattattt cccttctgta
     9541 tatagatatg ctaaatcctt acttccgcat attctctgag cgggtatgct acctgttgta
     9601 tcccaatttc atacagttaa ggacaggcca tgagtaagat ttttgaagat aactcgctga
     9661 ctatcggtca cacgccgctg gttcgcctga atcgcatcgg taacggacgc attctggcga
     9721 aggtggaatc tcgtaacccc agcttcagcg ttaagtgccg tatcggtgcc aacatgattt
     9781 gggatgccga aaagcgcggc gtgctgaaac caggcgttga actggttgaa ccaaccagcg
     9841 gtaataccgg gattgcactg gcctatgtag ctgccgctcg cggttacaaa ctcaccctga
     9901 ccatgccaga aaccatgagt attgaacgcc gcaagctgct gaaagcgtta ggtgcaaacc
     9961 tggtgctgac ggaaggtgct aaaggcatga aaggcgcaat ccaaaaagca gaagaaattg
    10021 tcgccagcaa tccagagaaa tacctgctgc tgcaacaatt cagcaatccg gcaaaccctg
    10081 aaattcacga aaaaaccacc ggtccggaga tctgggaaga taccgacggt caggttgatg
    10141 tatttattgc tggcgttggg actggcggta cgcttactgg cgtcagccgc tacattaaag
    10201 gcaccaaagg caagaccgat cttatctctg tcgccgttga gccaaccgat tctccagtta
    10261 tcgcccaggc actggctggt gaagagatta aacctggccc gcataaaatt cagggtattg
    10321 gcgctggttt tatcccagct aacctcgatc tcaagctggt cgataaagtc attggcatca
    10381 ccaatgaaga agcgatttct accgcgcgtc gtctgatgga agaagaaggt attcttgcag
    10441 gtatctcttc tggagcagct gttgctgcgg cgttgaaact acaagaagat gaaagcttta
    10501 ccaacaagaa tattgtggtt attctaccgt catcgggtga gcgttattta agcaccgcat
    10561 tgtttgccga tctcttcact gagaaagaac tgcaacagta atgccagctt gttaaaaatg
    10621 cgtaaaaaag caccttttta ggtgcttttt tgtggcctgc ttcaaacttt cgcccctcct
    10681 ggcattgatt cagcctgtcg gaactggtat ttaaccagac taattatttt gatgcgcgaa
    10741 attaatcgtt acaggaaaag ccaaagctga atcgatttta tgatttggtt caattcttcc
    10801 tttagcggca taatgtttaa tgacgtacga aacgtcagcg gtcaacaccc gccagcaatg
    10861 gactgtattg cgctcttcgt gcgtcgcgtc tgttaaaaac tggcgctaac aatacaggct
    10921 aaagtcgaac cgccaggcta gactttagtt ccacaacact aaacctataa gttggggaaa
    10981 tacaatgttc cagcaagaag ttaccattac cgctccgaac ggtctgcaca cccgccctgc
    11041 tgcccagttt gtaaaagaag ctaagggctt cacttctgaa attactgtga cttccaacgg
    11101 caaaagcgcc agcgcgaaaa gcctgtttaa actgcagact ctgggcctga ctcaaggtac
    11161 cgttgtgact atctccgcag aaggcgaaga cgagcagaaa gcggttgaac atctggttaa
    11221 actgatggcg gaactcgagt aatttcccgg gttcttttaa aaatcagtca caagtaaggt
    11281 agggttatga tttcaggcat tttagcatcc ccgggtatcg ctttcggtaa agctctgctt
    11341 ctgaaagaag acgaaattgt cattgaccgg aaaaaaattt ctgccgacca ggttgatcag
    11401 gaagttgaac gttttctgag cggtcgtgcc aaggcatcag cccagctgga aacgatcaaa
    11461 acgaaagctg gtgaaacgtt cggtgaagaa aaagaagcca tctttgaagg gcatattatg
    11521 ctgctcgaag atgaggagct ggagcaggaa atcatagccc tgattaaaga taagcacatg
    11581 acagctgacg cagctgctca tgaagttatc gaaggtcagg cttctgccct ggaagagctg
    11641 gatgatgaat acctgaaaga acgtgcggct gacgtacgtg atatcggtaa gcgcctgctg
    11701 cgcaacatcc tgggcctgaa gattatcgac ctgagcgcca ttcaggatga agtcattctg
    11761 gttgccgctg acctgacgcc gtccgaaacc gcacagctga acctgaagaa ggtgctgggt
    11821 ttcatcaccg acgcgggtgg ccgtacttcc cacacctcta tcatggcgcg ttctctggaa
    11881 ctgcctgcta tcgtgggtac cggtagcgtc acctctcagg tgaaaaatga cgactatctg
    11941 attctggatg ccgtaaataa tcaggtttac gtcaatccaa ccaacgaagt tattgataaa
    12001 atgcgcgctg ttcaggagca agtggcttct gaaaaagcag agcttgctaa actgaaagat
    12061 ctgccagcta ttacgctgga cggtcaccag gtagaagtgt gcgctaacat tggtacggtt
    12121 cgtgacgttg aaggtgcaga gcgtaacggc gctgaaggcg ttggtctgta tcgtactgag
    12181 ttcctgttca tggaccgcga cgcgctgccc actgaagaag aacagtttgc tgcttacaaa
    12241 gcagtggctg aagcgtgtgg ctcgcaggcg gttatcgttc gtaccatgga catcggcggc
    12301 gacaaagagc tgccatacat gaacttcccg aaagaagaga acccgttcct cggctggcgc
    12361 gctatccgta tcgcgatgga tcgtaaagag atcctgcgcg atcagctccg cgctatcctg
    12421 cgtgcctcgg ctttcggtaa attgcgcatt atgttcccga tgatcatctc tgttgaagaa
    12481 gtacgtgcac tgcgcaaaga gatcgaaatc tacaaacagg aactgcgcga cgaaggtaaa
    12541 gcgtttgacg agtcaattga aatcggcgta atggtggaaa caccggctgc cgcaacaatt
    12601 gcacgtcatt tagccaaaga agttgatttc tttagtatcg gcaccaatga tttaacgcag
    12661 tacactctgg cagttgaccg tggtaatgat atgatttcac acctttacca accaatgtca
    12721 ccgtccgtgc tgaacttgat caagcaagtt attgatgctt ctcatgctga aggcaaatgg
    12781 actggcatgt gtggtgagct tgctggcgat gaacgtgcta cacttctgtt gctggggatg
    12841 ggtctggacg aattctctat gagcgccatt tctatcccgc gcattaagaa gattatccgt
    12901 aacacgaact tcgaagatgc gaaggtgtta gcagagcagg ctcttgctca accgacaacg
    12961 gacgagttaa tgacgctggt taacaagttc attgaagaaa aaacaatctg ctaatccacg
    13021 agatgcggcc caatttactg cttaggagaa gatcatgggt ttgttcgata aactgaaatc
    13081 tctggtttcc gacgacaaga aggataccgg aactattgag atcattgctc cgctctctgg
    13141 cgagatcgtc aatatcgaag acgtgccgga tgtcgttttt gcggaaaaaa tcgttggtga
    13201 tggtattgct atcaaaccaa cgggtaacaa aatggtcgcg cctgtagacg gtaccattgg
    13261 taaaatcttt gaaaccaatc acgcgttctc tatcgaatct gatagcggcg ttgaactgtt
    13321 cgtccacttc ggtatcgaca ccgttgaact gaaaggcgaa ggcttcaagc gtattgctga
    13381 agaaggtcag cgcgtgaaag ttggcgatac tgtcattgaa tttgatctgc cgctactgga
    13441 agagaaagcc aagtctaccc tgactccggt tgttatctcc aacatggacg aaatcaaaga
    13501 actgatcaaa ctgtccggta gcgtaaccgt gggtgaaacc ccggttatcc gcatcaagaa
    13561 gtaattcttg ccgcagtgaa aaatggcgcc catcggcgcc attttttatg cttccgccag
    13621 cggcggcaaa atcaattcat cgctctcatg ctgctgggtg tagcgcatca cttccagtac
    13681 gcgcaacccc gcacggtgca ccgcatcgtt taacgccttc cctttcagca aaccactgat
    13741 aagctgagca caaaacaggt cgccagtccc tttcaggtcg gtttttaccc gtgaatggga
    13801 aatgacattc acgctgtcgg cactgaccac caccacctgc atctcctgat tttcttcatt
    13861 accggaggcg ctggtaatca ccacccattt taatgtgtct gaaagcagac tttttgcggc
    13921 agcaatggca ctgtcgagat cgcggcaatt tttaccggtc aggatttcca gctcaaagat
    13981 attgggggta attccctgcg ccagcggcag taaatattgt cgatacgcct cgggaaggtc
    14041 aggtttgaca taaattccgc tatcaatatc gccaatcacc ggatcgacca tgatcaatag
    14101 gtcaggatgg tctttgcgta gcgcagtcag ccactcggca aggattttga tttgcgatgc
    14161 cgttcccata tagcccgtgg ttacagcgcg cagctggcgc agcgcatcac gctcctgaag
    14221 cgcacgcaaa taaccgctaa accattcatc cggaatcgca ccaccgtaga aagtgtcata
    14281 atgcggcgta ttgctcagca ataccgtcgg cacggcaaaa acattcagac cgttctgttt
    14341 gatagcaggc acggcaatgc tgttgcccac gctgccgtaa accacctgcg actgcacggc
    14401 gacgatatcc gcctgcagcg ccctgctctt atcgttaaac aacaacaaac tactcattta
    14461 attttttctc cttgccgatg atcctcatcg tattccaacc gaaactttac ctgattctgg
    14521 cagtcaaatc ggctatcaca aaacaaggat aaggtaattc aatgaagaaa atcatttgtc
    14581 tggtcattac actactaatg acactccccg cttacgcgaa gttaactgcc catgaagaag
    14641 cccgcatcaa cgccatgctg gagggattag cacagaaaaa ggatttgata tttgtgcgca
    14701 acggtgatga acatacctgc gatgaagcgg tttctcatct gcgtctgaag ctgggcaata
    14761 cccgtaaccg cattgacact gccgagcagt ttattgataa ggttgcttcg tcgtcatcga
    14821 ttaccgggaa gccgtatatt gtgaagatcc ccggtaagag cgatgagaac gcacagcctt
    14881 ttttacatgc gctaattgcg cagacggata aaacggtgcc tgcgcaataa tcttaaatcc
    14941 ccgccccctg gctaaaatgc tcttccccaa acaccccggt ggaaaggtag cgatcgccac
    15001 gatcgcagat gatcgctacc accaccgcac cagggttagc ttttgccacc cgcagtgctc
    15061 cggcaaccgc gccgccggag ctgacgccac agaatattcc ttcccgcact gccagttcgc
    15121 gcatggtgtt ttctgcatcg cgctgatgaa tatccagcac ctcatccacc agagaagcgt
    15181 tgaaaatccc cggcagatat tccgcaggcc agcggcgaat gccgggaata ctgctgcctt
    15241 cttccggttg caggccaaca atggtcaccg gtttggattg ttcgcgcata aagcgtgaaa
    15301 cgccggtgat agtgccggtc gtccccatac tggagacaaa atgagtgatg cgcccgccgg
    15361 tttgctgcca gatttccggc ccggtggtgg tgtaatgcgc ataagggtta tcgggattat
    15421 tgaactgatc gagcagcttt ccttcgccac gattcgccat ctccagcgcc agatcgcgcg
    15481 caccttccat gccctgctct ttggtgacaa gaatcagttc cgcaccataa gcacgcatcg
    15541 ccgcacggcg ttcctggctc atgttgtcgg gcatcagcaa tttcatgcga tagcctttaa
    15601 gcgcggcaat cattgccagc gcaatgccgg tgttaccact ggtggcttcg attaagacat
    15661 cacccggttt aatttccccg cgcttttccg cctcgacgat catcgaaagt gccgcacgat
    15721 ctttcaccga acctgccggg ttattgcctt ccagttttaa ccacacttca ctgccgttat
    15781 ccggccccat tcgctgcaac ttcaccagag gcgtattgcc tattgtttgt tctaatgtac
    15841 tcacgatctc tatccatacg tggtgttgcc tgatgcgacg cttgcgcgtc ttatcaggtc
    15901 tacaggttac aaaccttgcc attaaaaagc ccggatcgcg ggaagcgcct ccgggcgttt
    15961 aacattcact caacctatca ggcgctttgt gcgagagcaa gttcctcatc gcgggtttcg
    16021 atacgctcgt cgccgttata cagccgcgca tgttgcagac caacgaataa acgctcgcca
    16081 cgctgcgggg catcgtcgcc atgcatcacg accgtcagcg gttcgttgta ccaccccagc
    16141 ggctgcacca ctaattgggt gtagtgacct ttcgggctgg cttccagtac ctgtaccggc
    16201 agcggcgaat cgagactggt acggcggctg atatccactt cccacgggcg caggaagaga
    16261 tccaccggcc cctgataagc aggtgtgtag cccagcggcc agcgatgtgc gccaacatgg
    16321 aactgcccgc cgcgaatggt tccctgcagg cggttcactt cgcccataaa ttcgagtaca
    16381 aaacgggtcg ctggttcgcg ccatacctga ttcggcgcat cagcctgttc aatattcccc
    16441 tgactcatca ccactacacg atcggctact tcggtcgcct cttcctggtc gtgggtcaca
    16501 aacacgctgg tgaatttcag ctcttcatgc agttgacgca gccagcgacg cagctcttta
    16561 cgcacctgcg catccagcgc gccaaacggt tcatcaagca gcagaatttg cggttctaca
    16621 gccagcgcgc gcgccagcgc cacacgctgt ttctggccgc cggaaagctg cgccggataa
    16681 cgatccgcca gatgcgcaag ctggaccatt tccagcaatt ttgtcacttt cgctttgatg
    16741 gctgcggcat tcgggcgctc gcgacgcggc agcaccgtca ggccaaaagc gatattgtcg
    16801 aataccgtca tatggcggaa cagcgcgtaa tgctggaaca cgaaaccgac cttgcgatcg
    16861 cgcgcgtgca ggcggctgac gtcggtgccg tggaagcgaa tatgcccgct ggtttgatgc
    16921 tccagcccgg cgataatgcg cagcagcgtg gttttcccgg aaccggacgg ccccagcaac
    16981 gcgaccatct gacctgaagg aatatccagt gagatatcgt tcagcacctg ggtgcgacca
    17041 aacgacttct taatattggc aatctcaatg ctcatgatgt tcctcctgct gtgcgcgttt
    17101 ttcctgattc tccaggcgcc actgcaacat actttttaaa aacagggtga taatcgccat
    17161 cagcgttaac agtgccgcag cggtaaagga gccgacggtg ttgtagtcct gctccagcaa
    17221 ttcaatctgt aacggcagcg aaagtgtttc gccgcgaatc gagccggaaa ccaccgacac
    17281 cgcgccaaac tcgccaatcg cgcgggcgtt ggtcagcact acgccataaa gcagcgccca
    17341 gcggatgttc ggcaatgtga cacgacggaa catctgccag ccagacgcgc caagcaaaat
    17401 cgccgcctcg tcttcctgac tgccctggct taacatcacc ggcaccagtt cgcgcaccac
    17461 aaacggacac gtcacgaaga tggtgaccag caccattccc ggccaggaga acataatttg
    17521 cagattatgt tcatccagcc aaccgcccag cgggccgtta gagccgtaga acagcagata
    17581 caccagaccg gcaaccaccg gcgatacggc aaacggaatg tccagtagcg tcagcagtaa
    17641 ctggcgtcca gggaagttaa agcgcgtcac cagccaggcc agcagaatgc cgaacaccag
    17701 gtttaccggt acggcaatca gcgcgatcat caccgtcagc cagatggcgt gtagcatgtc
    17761 cggatcggcc agattctgta aaaccggcat cagccctttg ctgaacgcct gtacgaagat
    17821 ataaatcatc ggcaccagca ggatgaacgc cgataccagc atcccgatgc caatcagaaa
    17881 ccatttgccc cagttaatcg ggcgcgcgtc ataacgcttc aattgggtaa cttccgccat
    17941 taatgaccta ccacacgccg accaaagcga ctttgcagag tgttaattga gaacagcagc
    18001 agcagagatg ccgcgaggat caccgaagca atcgcgctcg ctgccgggta atcaaactcc
    18061 tgtaagcgca caaaaatcat cagcgacgtc acttccgtct tccacgcgat atttccggcg
    18121 ataaaaatca ccgcgccaaa ttcaccaaga ctacgggtaa acgacagcgc cacgcccgcc
    18181 accagcgccg gagaaagctc cggcagcacc actttgcaga aactctgcca gcgcgttgca
    18241 ccaagcgttt ccgccgcttc ttcatattcc gggcctaact cttccagcac cggctgcacg
    18301 gtacgtacca caaacggaat gctggtaaag gccatcgcca ccgcaatccc cagccaggta
    18361 taggtgactt tgatatcaaa cttcgccagc cattcaccgt aaaaaccgtt tacagaaaag
    18421 agcgaggcta gcgttaaacc ggcgactgcc gttggcagcg caaagggtaa atccatcagc
    18481 gcatcaagca gcgtgcggcc tgggaagcga tagcgggtta ggatccacgc catcagcaga
    18541 ccgaaaacgc cgttaaaaat cgatgccaca aacgctgaca gcagcgttac tttgtaggcc
    18601 gctactacct gcgggttggt gatcacctcc cagtactgcg cccagctcat ctgagccagc
    18661 tgcatcacca gcgcggagag cggcagcagc aaaatcaggc acacaaacag cagactggtg
    18721 ccgaggctta aggtaaagcc cggcagcacg cgtcttgatg aaactgcaaa cattaattac
    18781 gccccgccgc taacagcttg tctaactcgc cgccgctggt gaagtgggtt ttcatcactt
    18841 ccggccagga gccaaatttg tcttccacgc ggaacagctc ggtctgcggg aatttgtttt
    18901 tcagtttgtc catgacttcc gggttattca cacggtagta atagtcggtg atgatggtct
    18961 gcgcctgtgg gctgtagagc cagttcaggt aggcttttgc ggctttttcc gtaccgttgg
    19021 cctgcacatt tttatcgacc cacgccaccg ggaattccgc cagaatattg gttttcggga
    19081 tcaccacttc aaagccctgt gcttcatact gtttacggat gttgttcact tccgattcaa
    19141 agctaatcag cacatcgccc aggccacgct cggcaaaagt ggtggttgcg ccacgaccgc
    19201 cggtatcgaa cacttcaacg tttttcagga actgggtcat aaactgttcg gttttggctt
    19261 tgtcaccacc gtcagcttta tccgctgcgc cccatgccgc cagataggta taacgcgcgt
    19321 tacccgacgt tttcgggttc gggaaaatca gcttcacgtc ggagcgcacc aggtcgttcc
    19381 aatcgtggat attcttcggg ttacccttac gcaccaggaa gcccatggtg gagtagaacg
    19441 gcgagctatt attcggcagg cgcgactgcc agtcggccgg gatcagcttg cctttatcgt
    19501 gcaggatttg tacgtcggtc acctggttat aagtgacaac gtcggctttt aagccctgta
    19561 aaatcgccag cgcctgtttt gatgacccgg catgagattg ttttatcgtc agtttgtcgc
    19621 cgccgttatc ttttgcccat tgttgctcaa acggcggatt cagggcggca aacagctcgc
    19681 gggagacgtc ataagaactg ttcagcagtt ccgttgcctg tacatggccc gccagcagca
    19741 gagaagcgac cagcgcgagt gagttctttt tcagtaagtt aacggccatt gcgcaccctt
    19801 ataaatttaa tgactttcta atagccatca tatttataac ggacgtgaaa ggagtaacgg
    19861 ttttatatac cgtttggtga tttggaagtt gaaaagggaa taagaaccgg gtttaaaacg
    19921 aaatcctccc cacacatgca gggaggaaac agaggtgaat cagataccga cgctaaccgt
    19981 ctccggcagt gtgctgccgc catcaatcac attctgtgta ccggttaaat agctggattc
    20041 atccgatgcg aggaaggccg ccagttcgcc gacttccagc ggatcggcga ggcgacgcat
    20101 cgggattgct ttcgccattt cagtcagcac cgactctgga tcttccgggt tcgactggcg
    20161 ggcaatgctt tccgccattg gtgtgcacac gtatcccggg caaatggcgt taacgcgaat
    20221 accagactgc gcgtactcca ccgccagcga ttttgtcagg ccaacaatcg ccgctttcgt
    20281 taaggcgtac gccgtttcgc caggatcggc caccatatcg ccagtgaccg aagacatcat
    20341 cacaatgcga ccatctttgc gggcaatcat ctccggcagc accgccttcg tgacgttcca
    20401 tacgccttta atattgatgt caatatggaa atcgcgatcg tcatcgctca tatcgaggaa
    20461 actgcccaga cgacaaacgc ctgcgttatt caccaggata tcaatgcgcc cttctttttc
    20521 cttcgcgcgt ttgatagctg cggctaccga cgccgggtca cgcacatcgg cgacaaccgc
    20581 cgtacagcga tgaccacgac cacacagttc gtccgccagc ttttcgatct caggggagat
    20641 atccagcaag attaggtttg cgccatgacg cgcaaaagtt ctggcaattc cttcgccaat
    20701 tccctgcaat gcgcccgtaa tcagtgctgt cttgcccgtg agtttaccca ttttaatgct
    20761 ctccttgtta atacagagcg ttacactcca ttccctctga aaatacagga taaacaaaat
    20821 attgctgcgg atcagctcac taattcagga aaatctcaat ccagtcgctg ctttatgcat
    20881 catttcaggc gttgtgttag ctcactgctg cgctgaatca ttttcaatga ctccacatca
    20941 ttgagttgca caagaccaac aaacagtaaa tcggtaacag aattttgcgc cgtgcgggta
    21001 gacatcgacg agctacgcca ttcggtttca ccggaaacgg tatcgagcgt gaaatgcgcc
    21061 agccgccgca gcggagaatc agccagagaa gtaatggcaa tcaccgttgc cccctgcttt
    21121 cgtgccgctt ctgcgcataa gacaatctct tttttactgc cgctgtaaga gatagcgatt
    21181 tgtacatcgc cttttttcaa cgcctgggaa acggtggctt gtacgtgcgt gtcggcttcg
    21241 caggccacgc gatatccaat tttcatcagt ttgaacgaca gatcgcgccc caccagcgcc
    21301 gatccgccca ggcccgttat ctgaataaat tgcgctttac tgatgacatc aatgattttt
    21361 tgcagccgcg cgtagtcgaa caacgcgcag gtttgctcca gcgccagctc tttttcgcga
    21421 ttcagtttgc gggcaatcac ttccagcgaa tcatcactgg taatcgaact gtgcaggtgc
    21481 agcgccgtgg cattcgtttt ctcccggctg gcactgtatt cgccaattaa cgccatacgc
    21541 aattcagtaa atccttgtgc gccgagcttc tgggcaaact tcacaatact cgactggctg
    21601 atgccgagct gtttcgccat cttgcgcgaa gagacggatt gcagctcact cacgttggcc
    21661 tgcagaaagt cagcgatttt ttgctcgttt tcggtgaatt cactccccgc attgcttatt
    21721 ttcgtcaggt acaacatctt ccactctcct gggctggcag cataaattcc gcttaagttc
    21781 ttatgacgct cttcacactc tgcgagtgta gactcaatga ttcctttaag tctgtcaatt
    21841 cgggatatat gattccatac atctgcaatt atttgatcgt aaatagtaag gtcaccaccg
    21901 atgcaactag aaaagatgat tactgaaggc tcgaacaccg cctcggctga aattgaccgc
    21961 gtatcgacgc tggaaatgtg ccggattatc aacgatgaag ataaaaccgt accgcttgcc
    22021 gttgagcgcg tcctgccgga tatcgccgcg gcgatcgatg ttatccacgc ccaggtcagc
    22081 ggcggcggtc gtctgattta tctcggtgcg ggaacatccg gtcgtctggg gattctggat
    22141 gccagcgaat gtccgcccac ctacggcgtg aaaccgggtc tggtggttgg tttgattgct
    22201 ggaggcgaat atgccattca gcacgcggtg gaaggcgctg aagatagccg ggaaggtggt
    22261 gttaacgatc tgaaaaatat taatttaacg gcacaggatg tggtggttgg cattgctgcc
    22321 agcggtcgca cgccgtatgt gattgccgga ctggaatacg cacgccagct cggctgccgc
    22381 acagtgggaa tttcctgtaa tccggggagt gccgtttcca ccaccgccga gtttgccatt
    22441 acaccgattg taggtgccga agtggtcact ggttcttcgc gaatgaaagc aggcacagcg
    22501 cagaaactgg tgctcaatat gctttccacc gggctgatga ttaaatctgg caaagtgttc
    22561 ggcaacctga tggtcgatgt ggtcgccacc aacgaaaaac tgcatgtgcg ccaggtcaat
    22621 atcgttaaaa acgccaccgg atgtaatgcc gaacaagcgg aagcggcgtt aattgcctgc
    22681 gagcgcaact gtaaaacggc cattgtgatg gtgctgaaaa atctcgatgc cgcagaagct
    22741 aaaaaacgcc tggatcaaca cggcggcttt attcgtcagg ttttagacaa ggaataaccc
    22801 atggccaaag agatcagcag tgaacttctg aacaccattc ttacccgtgt cggcggaccg
    22861 ggaaatatcg ccagttgtgg taactgtatg acgcgcctgc gtctgggtgt acatgacagt
    22921 tcactggttg atcccaatat caaaacgctg gaaggcgtga agggcgtcat tttgaccagc
    22981 gaccaggtgc aggtcgtttt tgggcctggt aaagcgcatc gtgccgcaaa agcgatgagc
    23041 gagctgctgg gcgaggcacc ggtacaggat gccgcagaaa tcgccgccca gaacaaacgt
    23101 cagttaaaag ccaaacaaac ctccggcgtg caacaatttc tcgccaaatt cgccaccatc
    23161 ttcacgccgc tgatccccgg ttttattgcc gccggtctgc tgctggggat agcgacgtta
    23221 attgccacgg tgatgcacgt tccggcagat gctcagggca cgctacccga cgcgctgaat
    23281 tttatgaagg tgttcagcaa aggtttgttc actttcctgg tgattctggt gggctataac
    23341 gccgcccagg catttggcgg cacgggcgtc aatggcgcaa ttattgccgc actctttttg
    23401 ctcggttata accccgctgc aaccaccggt tactacgccg gttttcacga tttctttggt
    23461 ctgcccatcg atccgcgcgg caatattatc ggcgtgctga ttgccgcgtg ggcctgcgcc
    23521 cgcattgaag gcatggtgcg ccgctttatg ccggacgatc tcgacatgct gctgacctcg
    23581 ttaatcaccc tgctgatcac cgctacgctt gcgtacctga tcattatgcc gctgggtggc
    23641 tggttattcg aaggtatgtc atggctgttt atgcacctga acagtaatcc gttcggttgt
    23701 gcggttttag ccgggctgtt cctgatcgcc gtggtatttg gcgtgcatca gggctttatt
    23761 cctgtttacc tcgcgttaat ggacagccag ggattcaaca acttatttcc catcctttca
    23821 atggcaggcg cgggccaggt gggcgcggcg ctggcactct actggcgggc gcaaccgcac
    23881 agtgcattac gcagtcaggt acgcggggcg attattcccg gtctgctggg cgttggtgaa
    23941 ccgttgattt acggcgtcac cctgccccgc atgaaaccgt ttattaccgc ctgtttaggc
    24001 ggcgcagcgg gcggtttgtt tatcggcctg atagcctggt ggggtctgcc gatgggctta
    24061 aacagcgcct ttgggccgtc tggtctggtg gcgctgccgc tgatgacttc cgcacaaggc
    24121 atccttccgg caatggcggt ttatgctggc gggattctgg tggcatgggt ttgcgggttt
    24181 attttcacca cgctctttgg ctgccgtaac gtcaatctgg actgatatca tgaaacggac
    24241 aatgctctat ctttctctgc tggctgttag ttgtagcgtc agcgccgcaa aataccctgt
    24301 tctgacagaa agttcgccag agaaagcagg gttcaacgtc gaacggctta accagatgga
    24361 tcgctggatt agccagcaag tggacgccgg ttatcccggc gtaaacctgc tgatcattaa
    24421 agataatcag attgtgtatc gcaaagcctg gggagcggcg aaaaagtacg atggcagcgt
    24481 gttgatggaa cagcctgtca aagccaccac cgggacgctg tatgacctgg cctcaaacac
    24541 caaaatgtac gccacgaact tcgccctgca aaagctgatg agcgaaggca aactgcatcc
    24601 tgacgatcgg attgcgaaat atattccggg gtttgccgat agtccaaatg acaccatcaa
    24661 aggcaaaaac acgctgcgga tttctgacct gctgcatcac agcggcggtt tcccggcgga
    24721 cccacaatat ccgaataaag cggtcgcggg cgcgttatat tcccaggata aaggccagac
    24781 gctggagatg atcaagcgca cgccgctgga gtatcagccc ggcagcaagc atatttacag
    24841 tgatgtcgat tatatgctgc ttggatttat cgtcgagtcg gtcaccggtc agccgcttga
    24901 ccgctatgtt gaagagtcga tttatcgccc gctcggcctg acacatacgg tatttaaccc
    24961 gctgctgaaa ggctttaaac cgcaacaaat tgccgccacg gaattgaacg gcaatacccg
    25021 cgatggcgtg atccatttcc cgaatatccg cacctccact ctctggggtc aggtgcacga
    25081 tgaaaaagcc ttttattcga tgggcggcgt ttccgggcac gcaggtttgt tttccaatac
    25141 cggcgatatt gcggtgttaa tgcaaacgat gctgaacggc gggggctatg gcgatgtgca
    25201 gctgttcagt gcggaaacgg tgaagatgtt caccaccagc tctaaggaag atgccacttt
    25261 tggcctcggc tggcgcgtga atggtaatgc caccatgacg ccgacgtttg gcacgctggc
    25321 aagcccgcaa acctacggtc acactggctg gaccggaacg gtgaccgtta tcgatccggt
    25381 gaatcatatg gcgattgtga tgttaagcaa caagccacat tcgccggttg ccgatccgca
    25441 aaagaatccc aatatgttcg aaagcggtca gctgccgatt gcaacgtatg gttgggtagt
    25501 tgatcaggtg tatgcggcgt taaagcaaaa gtaataaaaa ggccagtcag taatgttact
    25561 gcctggcctt taatcaatga atcagaaacg cttacagcgc catcaacttg tccagcgacg
    25621 gtgcgaaata atagccgccg gttaccggtt tggtgaaacg caacatcgca tcacgcttac
    25681 catcggtatc gccaaacatg ctcagcagtt gctgctcaat gttatgcaga cgcgcgcagt
    25741 aggcgcagaa gtacagaccg tgagtgccac tggcagtgcc gtacggcagg ctctggcgaa
    25801 caatcttcag ccctttgcca tcttctttca gatcaacgcg ggtgaggtga gaggtttccg
    25861 gacgttcgtc gccgtcgatc tcttcgttgg cctctttggt gcgcccgatc atcatctcct
    25921 gatcgtgaac gctcatccgg ttgagctgct tcaggttgtg ttcccaacgc tggacaaaca
    25981 catagctgcc gcccgcatcc acgccgtctt tgataaccgc cacttcgcga cgcgtctctt
    26041 cacccgccgg gttttccgta ccgtcaacaa agccgctcag gtcacgctct tcaacccaac
    26101 ggaagccgtg gatctcttct ttcacttcaa tgcagtcacc aaaggcttcc atcgccgcct
    26161 gggcgacaga gaagtttacg tcgtgacgca gagaaagaat gtggatcaac acatcgaact
    26221 gggtggtcgg cgcaaggcct ttaccgtagc ccggaaaatc tttcagctct tctgccccaa
    26281 cgccgccgct cagagcgcgc caggtgttgt taccaaaggc aaccaccgca ccaagatgcg
    26341 cgtccgggaa tttcgcttca aaagttgcca gtttgtcggc aaatgtttta ctggccgcac
    26401 gcagggcgtc aacttcccct ttcacgttgg cttcgatcca aatcgccgcg cggcaatgtt
    26461 ctggcaaaat gccactctga acctgagaca ttgttcctcc tgaaaataat aatgccacgc
    26521 aatccgtggc attgttgagc gctattttac gtgttttttc gcggcggcgg tttgttcaga
    26581 cgcaaattaa cgccgccaga taattttact gactttccag tttttcaggg tatcgtccga
    26641 aggcattaac ccttcaggac cactccattc cccgctgaac tggtagctaa tatgttgact
    26701 gccttcggct ttacattcga cggcacgatt atcatcacca tcagcttttt ggcaattgcc
    26761 aaatgctttg ctgtaaaggt cgctaaacgg tgtaccgatt ttaacaccgg tgtcagcagg
    26821 aatatcgcta tccagcacgt caatgcgact gatcgtgcct tgatcgccgt taatcaccat
    26881 cgccacgttg tcgcccttca tcacttcaaa aaagcgcacc acgttgccgt tcgcggtttt
    26941 cattccgctg cgcaggcgat aatcgccatc aagcgcatcg gcaatggctt gttcttgcag
    27001 tggtgtggac gccgttaatt caccaacgcc ctgctcgctc actttggtgg acgacccaaa
    27061 ccagttccac gggttagcgg ccgaccagtt aactgacgac atcgtggaac agccggtcag
    27121 catcaacggc atagcgcata acattaaacg cagcgatttc atgtcacttc ctttggttat
    27181 tcaataacgt tgcttggagt gcaaattcac caaaaagtgc cgttattctt tttcttcatt
    27241 aaaacaagcg cgtaaacgtc gattggtcag cagccagatt aacgccacaa tatccgccac
    27301 taccagcgcc agaccgatac cgctaacgga ttcaccgttc agccacagcc acggttgcca
    27361 gcaaagtaaa accacctgcg ccaacagcaa cagaaaatag agcacatgcc aagtgcgagg
    27421 aaatgtagcc cgccgaccgc tcaacaaaaa cgccagcacc gccggaatgc ctggaatcag
    27481 ccccagccag aaattatcgt gatcgggata aaacagattt agcagcgcag taccctgctc
    27541 gcgcgacgca ccggcaatga caaacagcac ccaggttcgc gcctgaagca atagcacaag
    27601 ccagaagagc aagggtaaac gcaggcgtcc gtgcgcatca taatggacag gatggaactc
    27661 agtactcttc atcttcaatc aaacgcttac ccagactcag cacgtcggcg tgttcatatc
    27721 ccaggcgttc atacattccg agcaccatgt cgttatcttc cggcacattg atctgaattt
    27781 tcgggcagcc acgagcaatc agctttttct ccagccgatt aagcaacgca ttggcaatcc
    27841 cacgcccacg aaactctgga tgcacgccaa gataataagc agacccgcga tgcccgtcat
    27901 aaccgcccat caccgttccg accacttcac cgtttacctc agcgaccaaa aacaaactga
    27961 cgtcatggtt catcttacgc tcgatgtcca tttccggatc gttccacgga cgcagcaagt
    28021 cgcaacgctc ccaaagggtg atgacctctt cgaaatcttc ctggcgaaat acgcgtatct
    28081 ccatggtatt cgttaccttt ttgcgggtta aaaggctgat tatggcgtga acggtcgaat
    28141 tagccaatat ctgacgaaaa tcggttgaaa aagtggcata atggggagtt gtcaactatt
    28201 gaaatgaaaa gtaaaacaat tctcaacatc aaaccgtcgt aacggattac gcgatacgat
    28261 ataacatctg gaactttatt attacaactc aggccgtatg agcactttta aaccactaaa
    28321 aacactcact tcgcgccgcc aggtgctgaa agccggtttg gctgccctga cgttgtcagg
    28381 aatgtcgcaa gccatcgcca aagacgaacc tttaaaaacc agcaacggac acagcaagcc
    28441 gaaagccaaa aaatctggcg gcaaacgtgt cgttgttctc gatccaggcc acggcggaat
    28501 tgataccgga gcgatcggac gcaacggttc gaaagaaaaa catgtggtgc tggcgattgc
    28561 taaaaacgtc cgttccattt tgcgtaatca tgggattgat gcgcgtttaa cgcgttctgg
    28621 cgatacgttt atcccacttt acgatcgcgt tgaaatcgcc cataaacatg gcgcagatct
    28681 gtttatgtca attcatgccg atggctttac caacccgaaa gctgccggtg cttcggtatt
    28741 tgccctctct aaccgtgggg caagtagcgc aatggcgaaa tacctgtctg aacgcgaaaa
    28801 ccgcgccgat gaagttgccg gtaaaaaggc gactgacaag gatcacctat tgcagcaagt
    28861 gctgtttgat ctggtgcaaa ccgatactat taaaaacagc ctgacgctcg gctcgcatat
    28921 tctgaaaaag attaagccgg tgcataaact gcacagccgc aacaccgaac aagccgcgtt
    28981 tgtggtgttg aaatcgccgt cggttccttc ggtgctggtg gaaacctcgt ttatcaccaa
    29041 cccggaagaa gagcggctgt taggcacggc ggcgtttcgt cagaaaatcg ccacagcgat
    29101 tgctgaaggc gtgatcagtt atttccactg gttcgacaac cagaaagcac attcgaaaaa
    29161 gcgataagtt atgaaacccg acgcacacca ggttaaacag tttctgctca accttcagga
    29221 tacgatttgt cagcagctga ccgccgtcga tggcgcagaa tttgtcgaag atagttggca
    29281 gcgcgaagct ggcggcggcg ggcgtagtcg ggtgttgcgt aatggtggtg ttttcgaaca
    29341 ggcaggcgtc aacttttctc acgtccacgg cgaggcgatg ccagcttccg ccaccgctca
    29401 tcgcccggaa cttgccgggc gcagtttcga ggcgatgggc gtttcactgg tagtgcatcc
    29461 gcataacccg tatgttccca ccagccacgc gaatgtgcgt ttttttattg ccgaaaaacc
    29521 gggtgcagag cccgtctggt ggtttggcgg cggcttcgat ttaacccctt tctatggttt
    29581 tgaagaagac gccattcact ggcaccgcac cgcccgtgac ctgtgcctgc catttggtga
    29641 agacgtttat ccccgttaca aaaagtggtg cgacgattac ttctacctca aacatcgcaa
    29701 cgaacagcgc ggtattggcg ggctgttctt tgatgatctg aacacgccag atttcgacca
    29761 ctgttttgcc tttatgcagg cggtaggcaa aggctacacc gacgcttatt taccaattgt
    29821 agagcgacgt aaagcgatgg cctacggcga gcgcgagcgc aattttcagc tctaccgtcg
    29881 cggtcgttat gtcgagttca atctggtctg ggatcgcggc actctgtttg gcctgcaaac
    29941 tggcgggcgc accgagtcga tcctgatgtc aatgccgcca ctggtacgct gggagtatga
    30001 ttatcagcca aaagatggca gcccagaagc ggcgttaagt gagtttatta aggtcaggga
    30061 ttgggtgtaa ctccctcacc cccattcccg catccgctga tgcaacgtca acgacggctt
    30121 ctcggcaaac agctgctggt aatccgtggc aaattgcccc agatgccaga atccccactg
    30181 catggcggcg tcttttaccg ttgtgctttg cgaccacggg cttatcagtt cgcggcgtac
    30241 ggcgttcagg cgaatgcgtt tcagccacgc gtttgggcca atgcctaaaa tagcgtgaaa
    30301 cgcgttttgt agcgtgcggc gactgacatg cagttgatta cacaagtcca gcaccgtcac
    30361 cggctcagac atattttcca gcacatattc acgggcgcgg gaaagtaatc tacggtaact
    30421 ctgatgactg atgctttccg ccgtcaccat tggttgcgcg tcttccagca tcgcccccat
    30481 cgccattagc aaattatccc ccagcacttt tcgcactgca ggctgatgga gattttccgg
    30541 gttctcgcaa aacgtcgcca gcgcctgttg gacaaagccc cacagcgcgg ctttatgctg
    30601 ctctttcact tccagcgccg actggctacg cagcatatgt aatacccgat ccgggttatg
    30661 caaaaagtta gcctgccggg tgatgacatc ttcagaaagc accacgccca ggatcgtgta
    30721 atcatccggc gtgctcagtt caaattcagt gccaccaggg cgggtggcga tttccgcgct
    30781 tcccagacat tgcgaaccga taaatccctg ctcaccgcgc gtcgccggaa tgccaaacca
    30841 gaacgagttc ggccagacca ggcacgactg acgcagcgcc agaccggtgt attcacgaaa
    30901 aacctgaata tcatcgagta gaatttccgt aaattcacca tgaaacttgc ccggatgcag
    30961 ctgatcgtaa atctgctgcc aggcggtaat cgttaaagca tgttcataga catccgttgt
    31021 ctgtcgttga tgaacattat ccacttcgac cttcggcgtg agcttcaggt tttcgggtaa
    31081 gggttcatga taaagatggt gcaaattggc tgtacgggtc tttttcatga tgttaatgcc
    31141 gggtgttgta ggacacccgg cacctccgac aggttaatgg ggcttgagac gataacgact
    31201 actgcgttta cataacgtcc cggcagaaaa gagctgttcg agcgcatttc tggctttttc
    31261 cagcggccag ccaaagtggg cagccacttc tcctgccgtc attccctgac gtactgatgt
    31321 taacagcgcc aacaactcgt cagcagattc cgcgacgata actggcgcgt cgggttccct
    31381 tacgggttgc ttcggctggc ggttaaagcc agtaaccagc cactgggtgt catcgtctgg
    31441 ccgaccgatc tccttgcggc tgatgacacg cccggtacgc atcgcggcag cacaacctgc
    31501 gtccagcgcc gcacgacacg ccgccagatc gccttccacc accagcgtta agcgaccagg
    31561 gtcaagcact tcgtgactga gcagacgaac gttagctgct ttgagcatgg catccgctgc
    31621 atctatcgcg gcgaccatgc cgtccacttc cagcaatccc agtgcattga tcatcggcaa
    31681 cctccgttac gcacgctgga ttggattacg cgcgatttcc agcactgcat cggtaaaggc
    31741 gttacaggct gctttacacg cggcctggct accggttaaa aatgctgccg agtagttggt
    31801 ttcagacggt ggcgggacat aggtcaccag ctgtacgtcg gcagatttca acgctgcatc
    31861 aatgccatag gtcgcttcca gcggcggtgc caccagatac gccatcggat cgccaagcgt
    31921 gatcccggcg gttgatgaga gataagaacc ggtacgcgaa accacatgcg ccaggaaggc
    31981 ggtgttttct gcgtcattgg cccactggaa tgctgcacca ttttcaatat gtgcaaccat
    32041 cgcatccaga ccggcacgca cttccgccgg gtttggccca ccaagcataa tcagcacttc
    32101 accggcagtc ggtgacgggc cgtgtgcagc gcccgcatac agcgagcgac catataccac
    32161 ttccaccatc gcctgcttgg tcgcttcatc agcggcaatg taggtgacgt catcggaatc
    32221 agcagaaatc agcccgaggc tacgaatatg cggcggcaat tttagttcac gcgcaaattc
    32281 agcgttaacg gaggcaatca cccgcatggc ggttaccgac ggtcgaatca aatctaaagc
    32341 tggcatgatg cctccttatc gggtcatgtt gatgccggat gctttctgct ccagcatacg
    32401 tttcgccaaa tccacaatga cggctgcggc ttcaaccggc ggcgtgccgc cctggtgaat
    32461 gttagagata caggtgcgat cggcctcaac ggtggtcgcc atacgcggcg agtaaacggc
    32521 gtagcaggag aggctttctg actgccctaa ccccggacgt tcccccacca gcaggatcac
    32581 cactttcgcg ccgaggatct caccgatctg atcttcaatc ttcacgcgac cataacgaac
    32641 aaagaatggc gtgccgactt tcagcccggc ctgtttcagg cccgccatca gcggcggcag
    32701 gatctcttcg tagttcacgg tgatggcatc agttgacagg ccatcagaaa taacgacctg
    32761 tacgtccgga ttagcaacac actgtgcttt cagcgcttca acagcttctg cacacaggcg
    32821 gcggcccata tccgggcgag tcaggtagag atttttgtcg ctaatttcag agcgtacttc
    32881 cagcaagccc tgcgctttca cccactcttc cggcacttct ttcagtacgg tatctttcga
    32941 acgagagtga tcggccagga aacgcagcag cgcctgggta cgtggacgcg gaccggcacg
    33001 accggtacaa acgcgggcca cggtgctgcg gcgcagttct gttaatacgt ctgcgcgatg
    33061 cgggttttca acaccaatcc acgcttttgc ttcagcggaa cctaaatcca gcgcacagct
    33121 ttccgaggtc accggtgccg cacagttggt ggtggcgcac tttgcttctg acggcgcggg
    33181 ggccgtttgt cccattgacg ccatcacgct gcgtacaatt tcttcaatct gtttttgatc
    33241 catgatgtgt tatctccgcg tcatcagaag aacagtgacg gatcgcccgc ccgtttggtc
    33301 aggcgaccgt ttgccataat gcccatgctt tccagccagc gttcaaactc tggtgacggg
    33361 cgcaggttga gtaactgacg cacagtggcg gtatcgtgga atgcggtggt ctggtagttg
    33421 agcatgatgt catcacccag cggcatcccc atgatgtagt tgcagcctgc ggtggcgagc
    33481 aggatcatca ggttttcgtt gaggttctgg tcagcgtcag cgtggttggt gtagcagcag
    33541 tcacagccca tagagatgcc gctcagcttg cccataaagt gatcttctaa gccagcacgg
    33601 ataatctggc ggtcgttgta gagatactcc ggcccaataa agccgaccac ggtgttgacg
    33661 ataaacggat cgtaatgacg cgccagccca tagttacgtg cttccatcgt cacctggtca
    33721 gcgccgaagt tagcgccagc ggatagcgca gagccttgtc cggtttcgaa gtagaggcag
    33781 ttttccccgg cgatacggtt gaactctgca cccactgcgc gcgcttcgtc gagcatcgcc
    33841 agttccacgc caaactcttt cagccctttt tcgctgccac agatactctg gaaaatcagt
    33901 ccgccaggcg cgccgcgacg aatcgcttcg atctgggtgg tgacgtgcgc cagtacacag
    33961 ccctgagttg ggatgttgaa tttgtcgatc acgccgtaaa tggtgtccaa cacgcggctt
    34021 aagttttcca cgtcgtcagt caccgggtta acgccgatca ccgcatcgcc caccccgaag
    34081 gaaagccctt cgtagatttg cgcggcgata ctttgcacgt cgtcacgggt gtcatttggc
    34141 tgcaaacggg cgctgaaggt gcccggaata ccaatggtgg tattggcctt tttgattacc
    34201 ggcattttct tcgcgccgta gatcaggtcc gcgttggagc aaatcttcgc taccgccgcg
    34261 accacttccg aggtcagccc tttgcgggta aaggcaatgt cgtccacgct ggtttcatcg
    34321 ctcagcacat actcacgcag ttcgctgatg ctccagtttt taatctggtt gtaggccgtt
    34381 tcgttaacat cgtcctgaat cagccgcgtc acgcagtcat cttcataggc aatcaccgga
    34441 ttattgcgga tgtccgctac ggtcatttcc gacaacacct gctttgccgc cacgcgctcc
    34501 tgtgagcttg ccgctgcaac gcccgccagc acatcccccg aacgcagttc gttggcttta
    34561 gccagcacct cttttacatc cttaaactga tatacattgc cgaacaatgt ggtctttagt
    34621 ttcataagtc gttccctcag gaaggaaatg cgagtgattt caccgtcacc ggcacaaccg
    34681 atccgccaaa aagaggcgta ccaatgtcga tatagtcccc cgcgcggaca atcacttcgt
    34741 caatgactgc caacgggagt tgttgtagct gtgggcgcaa caacatgccc agagctttac
    34801 caaagtcctg cccggccacc accagcaggg gatgcggatt cggaaaacgc gcgacgaaat
    34861 cgaccagcgc gttgatgacc gtcagtaccg cggcgtaacg cacaggcagc gaggcgggaa
    34921 gcgccagcac gtacgcgtca gttttgggat caagatccag ctgaagcagc gcctgttgcc
    34981 aggcactcac cagatccgtt tcatcaatcg ggatcgccac cggcaaattg cgcagcggca
    35041 gttgtacgcc ctccagccag attgtgctgc cagagagcga aagggtatgt gcacccgcgc
    35101 caatcaccgt ggcgcgtacg gtttgcgccg gaaactgcac attcatctca cgcaggcgcg
    35161 gatggtcatg cagcgccgtt gccagaagtg ggccaatatc ggcaaaacag aacgggtcgg
    35221 cgggctggtg gcgataacat tcgcccacgc ctccagaaag cgtaatgatt tcgggcgtaa
    35281 cacctgcggg cagcaaaccg gtttgcatca atgcctgcgc gagcggcgaa agcgttccgt
    35341 caatcacttc gacaatcagc gccgccatcc gccgggtaac ctgcaccagc tgcgcgccgg
    35401 tcagcgaacg ggcgtcagtg cctgcaccga agcactcatc cacaatcatc tgccccggtt
    35461 tatgagcgta aaccacgcgc ccctggctgt cggtttccag caggcgacca ccgacgttga
    35521 gacaggcagt gccgctgatt tttccggcat cgaacagggc gtagttcgcg gtgccaccgc
    35581 cgatgtcgat attcagtacc cgacacagcc gttgttcaga aagggtttgc gccccagctc
    35641 cgtgaccggc gatcacggat tcgaggtgcg gcccggcgct ggcaacgaca aaatccccca
    35701 gcgattgaga gagcgccatc accgccgggc gagcattgcg ggttttcgcg ctttcaccgg
    35761 tgatgatgat ggcaccagaa tcaacgcttt ccggcgcaat acccgcagcc tgatattgct
    35821 cgagtattaa ggttttcagt tccgcttctt ttaaaccgcc ctgtttatcg acaggggtaa
    35881 agaacaccgg actttgccag ctaatttcgc gtttaatgaa ttcgtagcgc ggcacctgcg
    35941 acaccgccgc acggttaacc agctccaggt gggagaaaat cacctgggtg gtggtggtgc
    36001 cgatatcgat accgacgctc aatagctggc gagtgttcac gattgtgcct ccgcttcggt
    36061 tttagtcgcg gtcgcgtctt cttttggcac cagcatcatc gccacgccaa tcgccgttac
    36121 gccgccgatc aacttgccga caatcatcgg gaagatcatg gcgttcatgt tggcagcggc
    36181 gaagcctaag tggtcgccca gggcgaaagc agcggaaacg gcgaaggcgc agttgatgac
    36241 tttgccgcgg gtatccatct gcttcatcat gccgaacatc gggatgttgt tggcaagcgt
    36301 tgccaccatg ccggctgccg cgatgttgtt catattcagc actttaccga cgctcatcag
    36361 cggtttttca aaccagcgag tcagcagcag caccattgga tacgccccta acagcacgca
    36421 ggagatagaa ccgataactt caatggcgcg catcacttca ccgggtttat cgccaggggc
    36481 cataaagata ggatccagtc cggggatcag ttcccagcca aggaggaatt tcactaccgc
    36541 agcggcaaga ccgagggtga tcaatgcaac gaggaatttg gcgaagatct ggaagccgtt
    36601 gatcattttt tccgggatga atttcagccc cagcgccacc agaatcgcga caatgatcac
    36661 cgggatcatg ttcatcagga tcagggcgaa agtgaattcc accggctgac cgttgatctg
    36721 cacaccggag tacatagcaa ccagaccacc agcaatacag ccaatcggaa tggtcacaat
    36781 gcccgccagc acgccgagcg ccagataacg acggtcagaa ggttcgataa tgccgagcgc
    36841 caccggaatg gaaaacacaa tcgttggccc catcatcgac ccgagaatta acccagagta
    36901 tagccacgct gctacgtcgc cgcccgccag ctctttggcg aggaagaagc cgcccatatc
    36961 gcacgccagc agtgttccgg cgaacatcga tgggttagcg ccgagcattt cgtaaaccgg
    37021 aataattacc ggcccgagta cgtgagccag taccggtgcc agcgcggtca taccgaccat
    37081 cgccaggccc agtgcgccca ttgccataaa gccttcttcg aactgaccgc ctgatccttc
    37141 gatactttta ccgaacttac cgaggaaacg agcagaaccg ccgaactgcg acaggatcct
    37201 gtctacggca gctatcagca taaagaacat catgatgtac atgatgattt cgttaattcc
    37261 catcgccttt actccctgtt agttgttatt tattggcgga tgcggcgtaa acgccttatc
    37321 cgccctacat gtgcaatccc gaaggccgga tcagacacgg caagcgtcgc atccggcatt
    37381 tattgcgccg ctgcgtacag gccgacaatc tgctctaggc tggcggtacg cgggttgctg
    37441 cgcagacaaa tatcttcctg cgcggcctgc gcccatgcac cgtaatgcgc agatgtcgcg
    37501 ccaacatcgc ccagtcgttt accaatccca acttccgcaa tcagctcact taccgcgtta
    37561 atagcgtcac gatcgtcgga ttttttagtt cgcagtgccc gaccaatctg actaaaccgt
    37621 tcgcgacaaa ccatccggtt aaattccatc accgttggca gcaacatggc gttcgccaga
    37681 ccgtgtggaa tatgcagcgc cgcccctggc tgatgcgcca tcgcgtggca caacccaagc
    37741 cccgcactgg aaaacgccat tcccgccata catgaagcca gtaacatgct ctcgcgcgcg
    37801 gcaaggtcat gaccgtagcc caccgctttc ggcagcgatt tgccaatcat cgcaatggca
    37861 ccaatcgcca gactgtcggt aaacggtgtg gcgttcagcg cgctgtacgc ttcaatggca
    37921 tgggttaacg catcaatgcc agtcatcgcc gtgacatgcg acggcacacc ttcggtcagc
    37981 gcggcgtcga ggatcgccac atccggcatc agcgaggcat gggctaacac ctgcttgcgc
    38041 ccgctcaccg tgtcgataat caccgttacg ttggtggttt cagagccggt tccggcggta
    38101 gttggaatgg caatcaacgg caggcgcggt tgcagaacgc tggtttctga catttccgcc
    38161 agcgtgctat ccgggttcgt caccagcaac gccacggctt tcgccgcatc cagcaccgag
    38221 ccgccgccaa atgcgatcac cccatcacag cctgactcac gcaactgcgc cacggctgca
    38281 cacacgtcgg tgatgcacgg ttcgcccacc ggacatggcc agagcgtcat ggcgatgcct
    38341 ttaacagcca ggctgcgcgt cagcccggcg gtcatccctg cctgatgcaa aaagctgtct
    38401 gccatcacga acagatgttt cagcccacgc gtttgcgctt gctgtccgca actgctcacc
    38461 gagcccggac cgcaaagcgt caccggtgga acgctaaatg tttttacccg ttgcagattc
    38521 agggtatcga acgcctgaaa gagcgcggtc tgcaattcat tttgcatata gcccctccgc
    38581 tttctctcgc ccactactgg cgatcgccag cggggtcata aacaaactgt gctgcggtaa
    38641 atgcacttgt aacgccggga attgtttgcg aaacagttcc gccacgcccg gttgcataca
    38701 ggagccgccc gccagccata aatcagtaat gccctgccct tcaatatgcc gggcgacaat
    38761 gtccgccatt ttttcgtaca ccggtttcac cgcaggccaa atctcttcac cgtgaccgcg
    38821 cttgtactgc tccgcctctt ccagcgaaat acggcgattt ccggcgagag tcagagagat
    38881 gtgatgtccg ccggtggctt catccgccga gtacgtcact ttgccctttt tcacgatggc
    38941 aatgccggta gtgccgccgc cgatatccac tacaccggcg ttgtccagtt gcagcagatc
    39001 cgcaactgcg gttggctcat ccagcacatg gctcacttcc agtccagcag actccagcac
    39061 gttgatggaa atacgcgggt ccgtacctgg aggaaatgaa gtcgccgcat ggctaaaacg
    39121 gcgaccgaat tgctgctcga gcgtgtcgag atggcgacga acaatggtga cagcgccgaa
    39181 gaaatcccag acgatgccgt cgcggaccac atcggcccag tcgaggcata ccgccaccgg
    39241 ctgaccgtcg cggtcgacaa ccatcgacac cacatcgcag gtgcccagat ccacccccag
    39301 ccacagcgga gattccgtcg cggcgggcgt ctggttacac agcgttgccg ccgtttgcag
    39361 acgtggggtg agccattgtt cgtcgtgcgc catcggttac tccttaaaca atgcgaaacg
    39421 catcgactaa tacacagcga cgcagacgga caaacgtccg cgcgctggtt accccttcac
    39481 cggttggcgt ggtgatggtc atggtggtcc agccttcccc gcccagcccc agcccggcaa
    39541 tgcacggtcc gttcttaacg aaaatgctgg tatcaatagc attcgccatc tggttcatgt
    39601 tttcgatgtt gcgcgagtgc attgccgccg tgtggtggca accgccttcc agtttcaccg
    39661 ccagcgcaat ggcatccgcc acgttggcga cgcgcacgac gggcagcacc ggcatcatca
    39721 gttcggtcac ggcaaacgga tgttctgcag tggtttccac aaacagcaaa cgcgtttctt
    39781 gcggcacgtt caggccgatt gccgccgcga ttttcgccgc atcgcgacca acccagtcac
    39841 ggctgacggt gccttttccg cgctcgtcga tatttttcag caacaccggt tgcagctgct
    39901 gcgcctgttc tgcggtcagt ttcaccgcgt gctggccttc catcagacgc atcagttcat
    39961 cggctacgct atcaacaaca atcagtacct tttcgtcggc acaaatgatg ttgttatcga
    40021 aagaagcgcc tttgacgatg gactgagcgg cacgggcgag gtcggcggtt tcatccacca
    40081 ctaccggcgg gttgccagcg cctgcggcaa tcagacgttt attggtgtgt ttacgcgccg
    40141 cttctactac cgcttcgccg ccggtcacca ccagaaggcc gatgcccgga aacttgaaca
    40201 aacgctgcgc ggtttcgata tccggatttg ccacagtaac cagtaagttt tccggcccgc
    40261 ctgcggcaac aatcgcctgg ttgagcagcg tgatcgcccg ctgggagact tttttcgccg
    40321 ccggatgcgg ggcaaaaatg acgctgttgc ccgcggcaat caggctgatg gcgttattaa
    40381 ttacggttgc cgccgggtta gtggacggag ttaccgaagc caccacgccc cagggcgcgt
    40441 tttcaattaa agtcaggccg ttgtcgccgg tcagcacctg cggagagagg cactcaacgc
    40501 ctggtgtgcc gcgcgcctga gcgacgtttt tggcaaattt atcttcaacg cgccccatgc
    40561 cggtttcact gacggcaagt tccgctaaat ctctggcgtg tttttcgcct gcttcacgaa
    40621 tggcagcaat ggctaactgg cgcattgcca cgctttttaa cccttgctgg gcgactttgg
    40681 ctgccgcaac ggcgtcatcc agggacgcga aaacgcccat ctcatgaacg gcggcggacg
    40741 gcgtgtcact gctttgcatt ttcagcagta ccgctttcac cacctgttca atatcctgtt
    40801 gattcatgat gttctgcctt atttgtggaa aattacctga ccgccagaca ccacctcatc
    40861 gacaatgcca atcacgcaca gatcgaccgg tgacgtttcg cttttatgcg cctggcgggc
    40921 ggaactgcca ctcaccagta acacccactc cccggttccc gcgccaatat tgtcgatggc
    40981 gacggcgcat tgcccgtcag gattaccttg tggatcaatc atttccacca tcagcaattt
    41041 gtcatgcgcc agtccgtgat ggcgtacggt acaaacaatt tgtccagtga cgactgccag
    41101 tttcataccc gcctccgtgg cgtatttcag gtaaaagctc cccctaccct ccgcagaagg
    41161 taaaatgaaa aaggagagag cgtgacgccc gaatcgacgt cacacagggt gattacaggt
    41221 tgctgctatc gcctttcagg ccgatcggga agacttcttc caggtcgccg tgcggacgcg
    41281 ggataacgtg tacagatacc agctcgccga tacgctgtgc cgctgcggca cctgcatcgg
    41341 tcgcggcttt acaggctgca acatcaccac gaaccatggc agtacacagg ccgccgccaa
    41401 tctgcttaac accaaccagc ttgacgcgcg cagctttcac catcgcgtca gaagcctcaa
    41461 tcagtgcaac caggccccgg gtttcgatca ttcctaatgc ttccattgtg ctttcctctt
    41521 tatcagggtc cagaacggga ccgttcattc aaccagtgtt tgtaaactgc tttcgcggtt
    41581 cacttctgtc tgacgcggca cagctgccac cagcgccagc tcgataattt cctgcacgct
    41641 acaaccacga gagagatcgt gcatcggcgc ggcaagtcct tgtatcagtg gcccgacggc
    41701 acgatatccg ccgagtcgtt gtgcgatttt gtaaccaata tttccggctt ccagcgacgg
    41761 aaaaaccatc acattggcct tgccctgtag cgggctggca ggcgcttttt gcgccgccac
    41821 ttccggcacg aaggcggcgt caaactgtaa ctcgccatcc accaccagct ttggtgcgcg
    41881 ctcacggacg atttctgtcg cctgctggac gttagcaaca caggggtgac gggcgctacc
    41941 attgctggaa aacgacagca tcgccacgcg cggctcttct ccggtgatgg cgcgccaggt
    42001 ttcggcactg gcaagcgcga tatccgccag ctgcgccgcc gtcggctgtg gcaccacgct
    42061 gcaatcggca aagcccaacg ccgggccgct gtactgtggc aacatcagga aaatggaaga
    42121 gagcgtttta cagcctggct gcaagccgat aatgcgtaat ccggcacgca gcacatttgc
    42181 cgtggaagag aggttgcccg cgatacagac atccgcttta ccggcgctga ccattgctgc
    42241 ggcgaacatc agagggtcgg taagtttttc cagcgcatcc ggcggcgttt tttcgcccgc
    42301 gcgggccagc cagcgatgag caaattcttc ccgcattgcg aggttgccat gcggatcaat
    42361 cacctgtagc ccgtccatcg ccacgccgtg actgagcgca aactgacgaa gttcaaacgg
    42421 attggcgacc agaatgggcg ttgccagacc ttgttgatgt aaatattgcg cggctttcag
    42481 cacacgttgg tctaacgcat ccggaaaaac cactctggcg ggcgctcgca acgccagttc
    42541 acgacaacgt tcaataatca ttgcgtttcc cccagtcgtt gttggatctg cttcaccgtt
    42601 agcggctgct tcgttacgct caggatcatc atcacgtaga ccgtgctcga aaggcggttc
    42661 agcgcctgca aaatgtccgg gcgtaatact tcaaagctgc gggtgataaa cacctgcgcc
    42721 gctacggttt cggtttcccg cactttggta cgtagcagat tgagcaacgc cgcatcgcgc
    42781 ccgtggctgg cttccggcac cagatgatcg tggtcgagat agcgcagcgg ctgatgagaa
    42841 agccgatgca gatcttcgtc agaaagcccg acaatcgcct ggcagcccag cggttccccc
    42901 agcgcatcgg cgcgcataat gttgccaaga cgcgaacgga tatccgctag ccacggctgc
    42961 cacggttccg ccagttcaat ttgcagccac actgccagcg caatggtgct gtcgagaacc
    43021 gcgcgaaaac ccagacgcgg atcgcttttg gcgaccattt tttccgccga caggtgagtc
    43081 agcgtatccg gcttttttgc caccggctgg cggcacagtt cgcagcacgc ctgcggatgt
    43141 tcatcgctac tggtcagccc atgaacgggc tgcggctgct gctgttcatc gtcaacaaac
    43201 aggcggccct gctcgtcaat aaacttgatg cgcagatggc ggctttccag caactcccgg
    43261 gcagagggcg tcaggcgact gtccgcaggg agatggatct ctgcgccttc gctgagcgta
    43321 tggttcgctc ttagccatgc ttcggtgatg aaatctttca tagggattgc cagttagccg
    43381 gccaggcgac atacaggaat ttcacgctgg atgtcgtacc aaattcgatg ctggagcctt
    43441 tcgggataaa catcacgtcg cctgctttgg cgatcatggt ttcgccttcg tggcgaacat
    43501 gtagctcccc ttccagcacc atgtcgattt cgtcgtagtt cagggtccac gggaagaatg
    43561 cgttctccca ctgcataaaa ccggcggcca tgctgctgcc gtcgtctccg gtcacaagat
    43621 cagtcaagcc cacgcaatgc ggctctgcgc cgtcgaaacg accaaacttg acgctgctgc
    43681 catcaatcac tttgatgccg cctttgccgg tcaccgattt gaagctcggc tgcattgcgc
    43741 cctgctccag cgactgtttt tccttcatca ctttttccat cagctgtgcg actaggcttt
    43801 cggtaaactg gccttccggc agctgggcaa tgatggtttc gcggatgcgc tggctttcgg
    43861 ttttgtcggc tggcacgctg gcgggcacgg acgccgttac cgggatcgat tcatcacatt
    43921 cggtgatggt aaaacccagt agatccgcca cttcacgggc ttccggggta ataatgctgg
    43981 cgcgcagaac cacggacatt gcctgttcgc cgcgcgcgtg tgcttcacga atatcattcg
    44041 ctgtgatgag ttttttcacc tgcttcctcc tgtttggtga gagatgccag ataatccacc
    44101 agttgctgca cgctttgcgg gtcgtggctg tttagctcga aaatcggctc ttcgaagcca
    44161 gtctcaagca ataactttcg cgttgcggcg acgtcagcat ctggcatgtc cgttttactg
    44221 atgacggcga tttgccgttt gctgacgcca atatccagca acccggcagg caaccgactt
    44281 tccgggtcat ttgctccatg gacatagatc agcatgtcta catcctgtag cgtggtaatt
    44341 aaggcgtgat accagcgggg atggctaaaa tattcacccg gcgtatcaat atcgccctta
    44401 tcattaaatt ccacggcctg tgtttttctg gcgagggtat aattcccctg caacgcatta
    44461 aataacgttg tttttcctgc cccgacgcta cccacaaacg caattcgttt catgaatgcc
    44521 tcgtattaac ttttggtcat ttcacaaagc gtataattta ataagcgacc taaaccgctg
    44581 acggtttgtg ataacgcttc ttctactgcg cccaccgagc cataaatcac caatgcaccg
    44641 ctgaagcggt cgagaaaacc gatatgcaca tcagcggctt tcagcgctaa atcaccggcg
    44701 atcatcgctg tttcgccggg agttagcgtc ataatgccaa ttgcgcccgc atccggaacg
    44761 ccgattttct tcgccagttc ctcaccggga tgcgcgatga gatgcgccag tgtgacctgt
    44821 ttgccgggca caaattcctg aatgatgcgt tctttatcca tgagtcgccg cctgtgaaaa
    44881 actttcgtgt atcgtgacaa tttttcgtca gagtaaataa caaaattcgg caagcgcgtt
    44941 taaaaggtga ggtagatcac taagaatgga gagatattcg ctgtggtgca taaactttca
    45001 tatgcaacgc atgaatataa ataaaataat atcgagatat tatatagact gcttttttta
    45061 ttattcgcaa actcagtaat aagctttact aatatattgc agccaaaccg cttaccctga
    45121 atattcaggg taagcgtgag agttaaaaaa gaattacagc ggttgggttt gcgcttcaac
    45181 cacggccagc gccaccatgt tgacgatacg acgcaccgat gcgatcggcg ttaacacatg
    45241 aaccggtttc gccacgccca tcagcaccgg accgacagtc acaccttccg agctggaaac
    45301 gcgcagtaag ttgtaactaa tgcgggcagc ttccatgttc ggcatcacca gaatattggc
    45361 agaacctttc aaagggctgt ccggcatacg gtcgttgcga atcgcttcca ccagcgctgc
    45421 atcgccgtgc atttcaccat caatcatcag atctggcgca cgatccctga ccagttccag
    45481 cgcctgacgc attttgctcg acgacgggca gtcagaagaa ccaaagttgg agtgcgacaa
    45541 cagagcaacg cgcggctcaa taccaaaacg acggacagtt tctgccgcca tcaaggtgat
    45601 ctccgccagc tcttctgcgt ctggttcgtc attgacgtag gtatcggcaa taaaggtgtt
    45661 accactcggc agcagcagcg cgttcattgc acctgcggtg tgaacgccat cgcgataacc
    45721 aaagacattt ttcaccacgc taaaatgttc atgataatcg cccaccgtac cgcagatcat
    45781 tgcatcggct tccccacgct gaaccatgat cgcgccgatc actgtcgggt tactgatcag
    45841 cgcccgctgc gcctgttcct gagtgacacc gcgacgcttc atgatctgga agtattcggt
    45901 ccagtactct ttaaagcgcg gatcggattc gttattgacg atctcaaaat caacgcccgc
    45961 tttgatctgc aagcccagtt tctgaatgcg catttcgatc acgttcggac gaccgataag
    46021 gatcggtttc gccagtccca gcgttaccag ttcctgagtg gcatgcagaa cgcgcgcctc
    46081 ttccccttct ggcagaacaa cgcgcttcgg cgctttgcga gcctgggaga aaatcggctt
    46141 cataaacagg ttggttttgt aaacgaactc agtcagcttg tcgatgtaga cgtcgaaatc
    46201 agcaatcgga cgcgtcgcca cgcccgactc catcgcggct ttagcgaccg caggagcgat
    46261 cttaacgatc aagcgcggat caaacggttt tggaatgatg tattccggac caaagctcag
    46321 atcctgatcg ccatacgctg aagccaccac ttcgctctgt tcggcgtggg ccagttccgc
    46381 aatcgcgcgt accgccgcca gtttcatctc ttcattgata gccgttgcgc caacatccag
    46441 cgcgccacgg aagataaacg ggaagcaaag gacgttgttg acctggtttg gatagtcaga
    46501 acgaccggtg caaatgatgg catccggacg cacttctttc gccagcggcg gcagaatttc
    46561 cggttccggg ttcgccagcg ccaggatcat tggcgcacga gccattttct tcaccatttc
    46621 ctgggtcagc actttcgggc cggaacatcc aaggaaaata tccgcgcctt caatcacatc
    46681 atcgagggta cgtttgccgt catccaccac cgcatatgcg gctttggttt ccgccatgtt
    46741 tggctcacgg ccctgataga taacgccttt tgaatcgcaa accacgatgt tatgtttttg
    46801 cagacccagc gctaccagca ggttcataca ggcgattgct gcggcacccg cgccggaaac
    46861 caccatccgc acgtcggaga tgtttttctc caccacgcgc aagccgttga ggatggcggc
    46921 agtgctgatg attgccgtgc cgtgctgatc gtcgtggaat accggaatat tcatccgctc
    46981 gcgcagtttc tgttcaatat agaaacattc tggcgcttta atgtcttcga ggttgattcc
    47041 gccgaaggtc ggttcgagcg cagcgacaac ttcaataaat ttgtccgggt cgagttcatc
    47101 aacttcaatg tcaaatacat caatcccggc gaatttctta aacagaacgc ccttgccttc
    47161 catcaccggt ttgccagcca gcgcgccaat gttgcctaac cccagcaccg ccgtaccgtt
    47221 agagatcacc gccaccaggt taccgcgcgc ggtatatttg taggctttta acgggtcttt
    47281 ttcgatttca agacaaggtg cggcaacgcc tggtgagtag gccagcgcca gatcgcgctg
    47341 cgttgccaga ggcttggttg gagaaacctg gattttccct ggaactggaa attcatggaa
    47401 atcaagtgca ctttgtttta actggtcatc catttggttg ttcctttcac gtaacgttca
    47461 caaataaagt gtgtgggcaa cagcccctgc ccacaacgtg gcgcacatta ttaccctgcc
    47521 ggagtctaca gactttgagc aagtccaaac tctcaccatt aatataatgt tttggtaata
    47581 atcctataac actgatgtta cctgcttaat ccagcaatac catgcctgtc tgctatgctt
    47641 ttttgatgcg tttagcgaaa tttctcagaa gtgtgaatta acgcactcat ctaacacttt
    47701 acttttcaag gagtatttcc tatgaacgag ttagacggca tcaaacagtt caccactgtc
    47761 gtggcagaca gcggcgatat tgagtccatt cgccattatc atccccagga tgccaccacc
    47821 aatccttcgc tgttactcaa agctgccgga ttatcacaat atgagcattt aatagacgat
    47881 gctatcgcct ggggtaaaaa aaatggcaag acccaggaac aacaggtggt cgcagcgtgt
    47941 gacaaactgg cagtcaattt cggtgctgaa attctcaaaa tcgtacccgg tcgcgtgtca
    48001 acagaagttg atgcacgcct ctcttttgat aaagaaaaga gtattgagaa ggcgcgccat
    48061 ctggtggact tgtatcagca acaaggcgtt gagaaatcac gcattttgat caaactggct
    48121 tcgacctggg aaggaattcg cgcggccgaa gagctggaaa aagaaggtat taactgcaac
    48181 ctgacgctgc tgttttcttt tgcccaggcg cgggcctgtg cagaagcggg cgtttttctg
    48241 atttcgccgt ttgtcgggcg tatttatgac tggtatcagg cacgcaagcc gatggacccg
    48301 tatgtggtgg aagaagatcc gggcgttaaa tcggtgcgca atatctacga ctactataag
    48361 caacaccact atgaaaccat tgtgatgggc gcgagcttcc gtcgcaccga acaaatcctc
    48421 gccttaaccg gctgcgatcg actgactatc gcaccgaatt tgctaaagga gctgcaggaa
    48481 aaagtttcgc cagtggtacg taaattaatc ccaccttctc agacgttccc acgcccagct
    48541 cccatgagcg aagcggagtt ccgttgggag cacaatcagg atgcgatggc ggtagaaaaa
    48601 ctgtctgaag gcattcgtct gttcgccgtt gatcaacgca aactggaaga tcttcttgcc
    48661 gccaaactat aaaccagcca cggagtgtta tatgtcccga aaagaccttg ccaatgcgat
    48721 tcgcgcactc agtatggatg cggtacaaaa agccaattct ggtcatcccg gcgcgccgat
    48781 gggcatggct gatattgccg aagtgctgtg gaacgatttt cttaaacata accctaccga
    48841 cccaacctgg tatgatcgcg accgctttat tctttccaac ggtcacgcgt cgatgctgct
    48901 ctacagtttg ctacatctga ccggttacga cctgccgctg gaagaactga agaacttccg
    48961 tcagttgcat tcgaaaaccc caggccaccc ggagattggt tatacgccag gcgttgaaac
    49021 caccaccggc ccgcttggac aaggtttggc gaacgccgtc gggctggcga tagcagagcg
    49081 tacactggcg gcgcagttta accagccaga ccatgagatc gtcgatcact tcacctatgt
    49141 gtttatgggc gacggctgcc tgatggaagg tatttcccac gaagtctgtt cgctggcagg
    49201 cacgctggga ctgggcaagc tgattggttt ttacgatcac aacggtattt ccatcgacgg
    49261 tgaaactgaa ggctggttta ccgacgatac ggcaaaacgt tttgaagcct atcactggca
    49321 tgtgatccat gaaatcgacg gtcacgatcc gcaggcggtg aaggaagcga tccttgaagc
    49381 gcaaagcgtg aaagataagc cgtcgctgat tatctgccgc acagtaattg gcttcggttc
    49441 gcctaacaaa gcgggtaagg aagaggcgca tggcgcaccg ctgggtgaag aagaagtggc
    49501 attggcacgg caaaaactgg gctggcacca tccgccattt gaaatcccta aagagattta
    49561 tcacgcctgg gatgcccgcg aaaaaggcga aaaagcgcag cagagctgga atgagaagtt
    49621 tgccgcctat aaaaaggctc atccgcaact ggcagaagag tttacccgtc ggatgagcgg
    49681 tggtttaccg aaggactggg agaaaacgac tcagaaatat atcaatgagt tgcaggcgaa
    49741 tccggcgaaa atcgctaccc gtaaggcttc gcaaaatacg cttaacgctt acgggccgat
    49801 gctaccggag ctgctcggcg gttcggcgga tctggctccc agcaacctga ccatctggaa
    49861 aggttctgtt tcgctgaagg aagatccagc gggcaactac attcactacg gggtgcgtga
    49921 atttggcatg accgctatcg ccaacggcat cgcgcaccac ggcggctttg tgccgtatac
    49981 cgcgacgttc ctgatgtttg ttgaatacgc ccgtaacgcc gcgcggatgg cggcactgat
    50041 gaaagcgcgg cagattatgg tttataccca cgactcaatt ggcctgggcg aagatggtcc
    50101 gacgcaccag gctgttgagc aactggccag cctgcgctta acgccaaatt tcagcacctg
    50161 gcgaccgtgc gatcaggtgg aagcggcggt gggctggaag ctggcggttg agcgccacaa
    50221 cggaccgacg gcactgatcc tctcaaggca gaatctggcc caggtggaac gtacgccgga
    50281 tcaggttaaa gagattgctc gtggcggtta tgtgctgaaa gacagcggcg gtaagccaga
    50341 tattattctg attgccaccg gttcagagat ggaaattacc ctgcaagcgg cagagaaatt
    50401 agcaggagaa ggtcgcaatg tacgcgtagt ttccctgccc tcgaccgata ttttcgacgc
    50461 ccaggatgag gaatatcggg agtcggtgtt gccttctaac gttgcggctc gcgtggcggt
    50521 ggaagcaggt attgccgatt actggtacaa gtatgttggt ctgaaagggg caattgtcgg
    50581 gatgacgggt tacggggaat ctgctccggc ggataagctg ttcccgttct ttggctttac
    50641 cgccgagaat attgtggcaa aagcgcataa ggtgctggga gtgaaaggtg cctgatggtg
    50701 atcgccggat gctgattgcc ggatgcgacg ctgacgcgtc ttatccggcc tacagcgtct
    50761 tatccggcct acatgtcccc gcaattttgt ttaacgggtg atccacaacg tgggccaggc
    50821 gtctggccca tgccagttat cgcaggtggg ttctgcggca taacgcacca ggcgaaaacg
    50881 ctgaccgtca aagcgccagc gcgcctgaat gccacaatcg cttaatccgc gccctttcgc
    50941 taaggtcacc agttcacgcg atttctcatc aaatgttgcg ttcatcagtt ccagttcatt
    51001 cgtctcctga ccgttgttga acggcaaacg caaccgaacc gggcgcgagg ctagtggctt
    51061 tttacgcgac acaatccatg ccaaatcaat ggtgttatag gcccctgcct cacagctaat
    51121 catcatcagc gctttatcat cagtcagcgc agtgacattc acctcacgac gtaacggatc
    51181 aagcgagcag cgcagaccat tcatccgcca gtttccataa tccagcaaat cgttgcgttc
    51241 ttcgagtgag agtggtgtcg gcgttgggtt aaccaccgcg acctctttca gcgcaggcgc
    51301 gggcggtacg ctgagcggcg gttcgtcccc tttcttgatc cacgcggttt cactgccaac
    51361 gcgcttttgc tgagcatcaa taaacaacaa cgccgctttt aagccactca gagaaatggt
    51421 ctgattgccg tcgcgtaagg tgatcgcctt cccttcctga atcatctgca aaaacgcggt
    51481 gatggttgcc gtatcatcgg ttactaataa ccatggtgaa atccgccact tgtcaccact
    51541 taacgccaac ggctcaccat ctaacagcag ccgtggcgct atctctcctt cagacgcatc
    51601 cggcgacttc aacccgccgc gctcaatacg taaaacggca tcggtatgcg ccccggcgct
    51661 gcggctcagg gtcatcacca gtccattatg atcgcccgta ttgcgcgcca cgcagaagtt
    51721 ttggttatta caggtgacct gccagtcgga aaacgcccgt tgcgctggtg ccgcccacac
    51781 caaagacgtc ggcaacaaag cgaaaaaaaa gagaaggaaa atgcgatagc gcatggacgg
    51841 tacgacccca gaagttcaaa caaacagcac aagtcgtatc ttcgttgcgc aggtagcgcg
    51901 gctcaatcag atttgtcgga ttgagccaat aataaacaac gaaatattat tgttattttt
    51961 cagtccatta aatgtgacgt ttgcaaatag ttaagcaata acaccgtctt accgtcacgt
    52021 atctcgccgg ttttgatcat ctccagcgcc tggctgaacg gcagctcgag cacttcaata
    52081 tcttcatctt caacaccgcc accggcgtta gcgcgctgat tgtcactgta ttcggcgata
    52141 aaaaagtgga ttagctcagt cacaccgcca ggcgacatat acagttcaaa taatttgcgc
    52201 acttcgccca cttcatagcc cgtctcttca atcgcttctt tacgaatgca cacttccggt
    52261 tcgtcgttat ccagcagccc ggcgcaggtt tcaatcagct gcccgctttc attgccatta
    52321 acccaggtag cgacacggaa ctgacgaatc agaaccacgc tctttttctt cgcgttgtac
    52381 aggaggatcg tcgcaccatt accgcgatcg taaacttcac gtttatggcg gataacttcg
    52441 ccatctttgc gcgtgagatc gtaggtaatg ttgtgcaggg tgaaatagtt atcggagaga
    52501 attttgtctt taatgagggt gatttgttgc gtcataccga ctccacagcg cgaaatgaac
    52561 aattatctta cgctgtgaag tcggttttgt ctgcgcaggc tatcagtgag atttgactga
    52621 ttttaccccc agccagttaa taatcccctg tgccgcatga cgtccttctg ccattgcggt
    52681 aaccaccaga tccgcaccac gcacggcgtc accaccagcg aagattttcg gattggtggt
    52741 ctggtaacgg tactggcttt ccacatccgc gatgatgcgg ccccatttgt ctaccgttac
    52801 accgtgcgac tccagccacg gcatcccgtg cggattgaag ccaaacgcca taatcaccgc
    52861 gtcggctggc atgacaaatt cactgccttc caccggcact ggacgccgac gcccctgggc
    52921 atccggctcg ccaagacgcg tgcgcaggaa acgaatcccg cagacgtgac cttgttcatt
    52981 cagctcaagc gccaccggct ggacgttaaa ttcgaagttg gccccctctt cgcgggcgtt
    53041 cttcacttct ttcttcgagc ctggcatgtt agcttcatca cgacgataag cgcaggtgac
    53101 gttactcgcg ccgtggcgca gtgcggtacg cacacagtcc atcgcggtgt cgccgccgcc
    53161 cagtaccacg acgttaagtc cggcggtatt gataaacggc tcttccggta gctcttcgag
    53221 ccccatcacc tgtttagtgt tggcaatgag gaacggcagc gcgtcataaa cgcccggcgc
    53281 atcttcattg ggtaaacccg ctttcatgga acggtaagtg cctacgccaa cgaagaccgc
    53341 gtcgtattgt tccaaaagcg aatccaaaga gacatcttta cccacttcac aattgagttc
    53401 gaagtgaatc cccatcgcgc tgaagatttc ccgacggcgt gccagcaggg atttatccag
    53461 tttgaaagaa ggaatgccga aagtgagcaa gccaccgatt tctggatggc gatcgtacac
    53521 cgtcaccccc acgccattgc gggtcagaac atccgcacag gccagccctg ccggacctgc
    53581 accgataatc gccacccgct tgtccacttt ggtgacatgg cttaagtcag gacgccaacc
    53641 tttcgccaac gcctgatctg aaatgtagcg ttcaatgttg ccgatagtta ccgcgccgtg
    53701 ctcatcgcga atagtacagg caccttcaca caaacggtct tgcggacaaa cgcgtccggt
    53761 aatttccggc agggtgttgg tctggtgaga aagctcgacg gcggcgtcga tgtttccggc
    53821 tttcaccagt tcaatccact gcggtatatg gttatgcagc gggcaggtcc attcacaaac
    53881 gctgtgctcg ccgcacttaa ggcagcgcga ggcttcccgt tgtgcctggt cggcgcgaaa
    53941 tggcagataa atttcatcaa aaccggtttt gcgcgcttca atcgccagtt tatccggctc
    54001 gccacgcgcg ggcgttgcct gcatttgttc gactttactc attaccggca tttcttgcgc
    54061 cgcggtactg gcatgccacg gttgatgttc ctgacgcgcg gtgcgcaagc ggcgggattt
    54121 cgccatgccg gagagtgcga cgtcagtgac cagttgcagc gcgtccgccg ggcaattctc
    54181 aacacaggca ggaccgtttt cgcgccccgc acaaaggtcg catttatgcg ccgtggcttt
    54241 tacttttcct gccgcgacgg gtgtcaggac gatttgcatc gtaccaaaag gacaggccac
    54301 cacgcaggat ttacagccaa tacacttttg ctgattgacc tgaatgctgt catcaacgtg
    54361 gctgattgcg ccattagggc agctacgggc gcagggcgca tcttcacaat ggtgacaggt
    54421 cactgcacta cgttgctgtt gatgtttgat aaccgtaatt cggggatgaa aatggtgttg
    54481 gctcaggaca tgttgctcat cattgtgagc catgacacag gcgatttcac aagcatgaca
    54541 acccagacat tgctgactgt tggccataat aaaacgattc ataacgacct tcttttttgg
    54601 ttgtaaaaac cttattcttt atatgagtgt tgttattacc cgacttacag gggaatcggc
    54661 aatgttcatg tgcccagaat aagtaactat ttcgctataa actgtggcag atcaaataat
    54721 cccatcactg actaaattgt gtttcagcga actggaacat taatgatttt ttgtggagaa
    54781 gacgcgtgtg attgttaaac gacccgtctc ggccagtctg gcccgggcct ttttttacat
    54841 tgtgctgctg tcgattcttt ccacgggtat cgctctgcta actctggcga gcagtttgcg
    54901 cgacgctgag gctatcaata ttgccggatc gctacgtatg cagagttacc gcctgggcta
    54961 cgacttgcaa agtggcagtc cacaactcaa tgcacatcgc cagttgtttc agcaggcact
    55021 gcattcaccg gtattaacca atctcaacgt ctggtatgtg ccagaagcag taaaaactcg
    55081 ctatgcgcat ctgaatgcca actggctgga gatgaataat cggctcagca agggcgattt
    55141 gccgtggtat caggccaata ttaataatta tgttaatcag atagacctgt tcgtgctggc
    55201 tttacagcac tacgctgaac gcaaaatgct gctggtggtg gcgatttccc tggctggcgg
    55261 tatcggtatt ttcacgctgg tcttttttac tttgcgccgt atacgccatc aggtggttgc
    55321 cccgctgaat cagctggtta ccgccagtca acgtattgaa cacgggcagt tcgactcgcc
    55381 gccgctggat accagtctgc cgaatgagct tggtctgctt gcaaaaacct ttaaccagat
    55441 gtcgagcgag ctgcataaat tgtacctttc gctggaggcg tcagtagaag aaaagacccg
    55501 cgatctccac gaggccaagc gtcgtctgga ggtgttgtat caatgttccc aggcgctgaa
    55561 caccagccag attgatgtgc attgtttccg ccatattttg cagattgttc gcgacaatga
    55621 agcggctgaa tatctggagt taaatgtcgg tgaaaactgg cggattagcg aagggcaacc
    55681 aaacccggaa ttgccgatgc agattttacc ggtgacaatg caagagacgg tttacggcga
    55741 actgcactgg caaaatagtc acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc
    55801 gatgctggga cgcggtttgt actttaatca ggcgcagaag cattttcagc agttattgtt
    55861 gatggaagaa cgtgcaacca tcgcccgcga attgcacgac tcgctggctc aggtactttc
    55921 ttacttacgt atccagttga cgttactgaa gcgttcgata ccggaagata acgccaccgc
    55981 acaaagtatc atggccgatt tttcccaggc gttgaatgat gcttatcggc agttacgcga
    56041 gctgttgact actttccgcc tgacgctgca gcaggcggat ctcccctccg cgttgaggga
    56101 aatgctggat acgttacaaa atcaaaccag cgccaaactg accctcgact gccgtctgcc
    56161 aaccctggcg ctggatgcgc aaatgcaggt gcatttgttg caaattattc gcgaagcggt
    56221 gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc gtcagttgcg tcaccgcgcc
    56281 ggacggcaat cacacggttt atatccgtga taacgggatt ggtatcggtg aaccgaaaga
    56341 acccgaaggt cattatggtc tgaatatcat gcgcgaacgc gcggaacggc tgggtgggac
    56401 gctgactttt tcacaacctt ccggcggcgg cacgttagtg agtattagct ttcgctctgc
    56461 ggagggtgag gaaagtcagt tgatgtaatg cctcctactg accaaagaat acttgcactt
    56521 aaggttcagt ataaaagggc atgataattt acattaactc ctttttttct ccacgattgg
    56581 ctcgtacctt gccgctacag tgaagcaagt caagcctaca acgatacgca gaaacacgag
    56641 gtcctctttt aatggcgaat ttctttattg atcgccccat ttttgcctgg gtgctggcaa
    56701 tcctgttgtg tctgacaggg accctggcga ttttttcatt gcccgttgaa caataccccg
    56761 atctcgcgcc accgaatgtg cgagtgaccg ctaactatcc cggcgcatcg gcccagacgc
    56821 tggaaaacac cgtgacccag gttatcgagc aaaatatgac cggcctcgat aatctcatgt
    56881 atatgtcatc tcagagcagt ggcaccggtc aggcatctgt cactttaagt tttaaagcag
    56941 gcaccgatcc ggacgaagcc gtgcagcaag tacaaaacca gctgcaatca gccatgcgaa
    57001 agttaccgca ggcggtgcaa aatcagggcg tgacggtgcg taaaaccggc gataccaata
    57061 ttctgaccat tgctttcgtc tctaccgatg gttcgatgga taaacaggat attgccgatt
    57121 atgttgccag taatattcag gacccgttaa gccgcgtgaa tggcgtcggg gatatcgatg
    57181 cctatggttc gcaatattcc atgcgtatct ggctggaccc ggcgaaactc aacagtttcc
    57241 agatgacggc taaagatgtc actgatgcca ttgagtcaca gaacgcgcag attgcggttg
    57301 ggcaacttgg tggtacacct tccgtcgata agcaggcgct caacgccacc attaacgccc
    57361 agtcactgct gcaaacacca gaacagttcc gcgatatcac cttgcgggtg aatcaagacg
    57421 gctcagaggt aaggctgggc gatgtcgcca ccgtcgaaat gggggcggag aaatacgatt
    57481 atcttagccg cttcaatggt aagccagcct ccgggctggg ggtaaaactg gcctccggcg
    57541 caaacgaaat ggcgacagcg gagctggtgc tcaatcgtct cgacgagctg gcgcagtact
    57601 tcccgcacgg actggaatac aaggtggcgt atgaaaccac ctcgtttgtt aaagcctcca
    57661 ttgaagacgt ggtgaaaacg ctgctggaag ctatcgctct ggttttcctc gttatgtatc
    57721 tgttcctgca aaacttccgc gccacgctga tacccactat cgccgtgccg gtggtgttga
    57781 tgggaacctt ctccgtactt tacgcctttg gttacagcgt caacacctta accatgttcg
    57841 cgatggtgct ggcgatcggt ctgctggtgg atgacgccat cgtggtggtg gaaaacgtcg
    57901 aacgtattat gagtgaggaa ggactcactc ctcgcgaagc cacacgtaaa tcgatggggc
    57961 agatccaggg ggcactggtc gggattgcga tggttctttc ggcggtattt gtaccaatgg
    58021 ccttcttcgg cggcaccacc ggtgccatct atcgccagtt ctctattacc attgttgcgg
    58081 cgatggtgct gtcagtactg gtagcgatga tcctcactcc ggctctgtgt gccacactac
    58141 ttaagccact gaaaaaaggt gagcatcacg ggcaaaaagg cttttttgcc tggtttaacc
    58201 agatgtttaa ccgcaacgcc gaacgctacg aaaaaggggt ggcgaaaatt ctccaccgta
    58261 gcctgcgctg gattgtgatt tatgtcctgc tgcttggcgg catggtgttc ctgttcctgc
    58321 gtttgccgac gtcgttctta ccgctggaag accgtggcat gtttactacc tcggtacaat
    58381 tgcccagcgg ttcaacccaa caacagaccc tgaaagtcgt tgagcaaatc gagaaatatt
    58441 acttcaccca tgaaaaagac aacatcatgt cggtgtttgc caccgttggt tctggccctg
    58501 ggggcaacgg gcaaaacgtg gcgagaatgt ttatccgcct gaaagactgg agcgaacgcg
    58561 acagtaagac cggcacctcg tttgccatta tcgagcgtgc aacgaaggcg tttaaccaaa
    58621 ttaaagaagc tcgcgttatc gccagcagcc cgccagcaat tagcggtctt ggtagttctg
    58681 cgggttttga tatggagttg caggaccacg ctggagcagg tcacgatgcg ctgatggcag
    58741 cacgtaacca gttgctggcg ctggcggcgg aaaacccgaa gctaacccgt gtgcgccata
    58801 acggcctcga tgacagtccg cagttgcaga ttgatatcga ccagcgtaaa gctcaggcgc
    58861 tgggcgttgc tatcgacgat attaacgaca cactgcaaac cgcctggggt tcgagctatg
    58921 tgaatgactt tatggatcgc ggtcgcgtga agaaagtcta tgtgcaggca gctgcgccgt
    58981 atcgcatgct gccagatgac attaatctct ggtatgtccg aaataaagat ggcggcatgg
    59041 tgcccttctc tgctttcgca acctcacgct gggagacagg ctcaccgcgt ctggaacgct
    59101 ataacggtta ttctgcggtt gagattgttg gagaagccgc accgggggtc agtaccggta
    59161 cggcgatgga tattatggaa tcgttagtga agcagttgcc aaatggcttt ggtctggagt
    59221 ggacggcgat gtcgtatcag gagcggcttt ccggcgcgca ggctccggcg ctgtacgcca
    59281 tttccttgct ggtagtattc ctgtgtctgg cggcattgta tgaaagctgg tcggtaccgt
    59341 tctcggtaat gctggtcgtg ccgctggggg taatcggcgc gctgctggca acctggatgc
    59401 gcgggctgga aaacgacgtt tacttccagg tgggcctgtt aacggtcatt ggtttatcgg
    59461 cgaaaaacgc catcctgatc gtctagtttg ctaacgagat gaaccaaaaa ggccacgacc
    59521 tgtttgaagc gacgctccac gcctgccgtc agcgtttacg cccgattctg atgacctcgc
    59581 tggcatttat cttcggcgta ttgccaatgg caaccagcac gggtgccggt tccggtggtc
    59641 agcatgcggt gggtactggc gtaatgggcg ggatgatttc agctactatt ctggctattt
    59701 acttcgtgcc gctgttcttt gtgctggtgc gccgccgctt cccgctgaag ccgcgcccgg
    59761 aataagcaat aaaaaaggcg acatgccaat gtgtcgcctt tttcaacttt ccgattaaga
    59821 acctgctcag cgggttcttg ctgtttatac tttgtctcgg gaattactta cgaagcataa
    59881 cttcgataaa gtctttccag ttccccagtt cacgttcaat cataacaacc tctcttataa
    59941 ttatgggtat tctacggaaa caatataccg tggtgaagct aatttactcg attgctgcga
    60001 tgactacctc cgggggacaa accttatgta aatactatgg tcctgcggtg ataatttgta
    60061 tgtgatacac agcaacattt cgagatattc
//
LOCUS       DAZGVQ010000007        58126 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000007 DAZGVQ010000000
VERSION     DAZGVQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.007"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            122..2656
                     /gene="mrcB"
                     /locus_tag="ACHLAG_000408"
     CDS             122..2656
                     /gene="mrcB"
                     /locus_tag="ACHLAG_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFP0653716.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDSRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2876..5119
                     /gene="fhuA"
                     /locus_tag="ACHLAG_000409"
     CDS             2876..5119
                     /gene="fhuA"
                     /locus_tag="ACHLAG_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFP0653717.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            5170..5967
                     /gene="fhuC"
                     /locus_tag="ACHLAG_000410"
     CDS             5170..5967
                     /gene="fhuC"
                     /locus_tag="ACHLAG_000410"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFP0653718.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5967..6857
                     /gene="fhuD"
                     /locus_tag="ACHLAG_000411"
     CDS             5967..6857
                     /gene="fhuD"
                     /locus_tag="ACHLAG_000411"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFP0653719.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPLLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6854..8836
                     /gene="fhuB"
                     /locus_tag="ACHLAG_000412"
     CDS             6854..8836
                     /gene="fhuB"
                     /locus_tag="ACHLAG_000412"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFP0653720.1"
                     /translation="MSKRIALFPALLLALLVIAATALTWMNFSQALPRSQWAQATWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQIR"
     gene            complement(8871..10151)
                     /gene="hemL"
                     /locus_tag="ACHLAG_000413"
     CDS             complement(8871..10151)
                     /gene="hemL"
                     /locus_tag="ACHLAG_000413"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFP0653721.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            10317..10364
                     /gene="yadX"
                     /locus_tag="ACHLAG_000414"
     CDS             10317..10364
                     /gene="yadX"
                     /locus_tag="ACHLAG_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFP0653722.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            10376..11797
                     /gene="clcA"
                     /locus_tag="ACHLAG_000415"
     CDS             10376..11797
                     /gene="clcA"
                     /locus_tag="ACHLAG_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFP0653723.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            11821..11886
                     /gene="yadW"
                     /locus_tag="ACHLAG_000416"
     CDS             11821..11886
                     /gene="yadW"
                     /locus_tag="ACHLAG_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFP0653724.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            11879..12223
                     /gene="erpA"
                     /locus_tag="ACHLAG_000417"
     CDS             11879..12223
                     /gene="erpA"
                     /locus_tag="ACHLAG_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFP0653725.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(12270..12893)
                     /gene="yadS"
                     /locus_tag="ACHLAG_000418"
     CDS             complement(12270..12893)
                     /gene="yadS"
                     /locus_tag="ACHLAG_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFP0653726.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(12931..13731)
                     /gene="btuF"
                     /locus_tag="ACHLAG_000419"
     CDS             complement(12931..13731)
                     /gene="btuF"
                     /locus_tag="ACHLAG_000419"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFP0653727.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLQDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(13724..14422)
                     /gene="mtnN"
                     /locus_tag="ACHLAG_000420"
     CDS             complement(13724..14422)
                     /gene="mtnN"
                     /locus_tag="ACHLAG_000420"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFP0653728.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            14506..16023
                     /gene="dgt"
                     /locus_tag="ACHLAG_000421"
     CDS             14506..16023
                     /gene="dgt"
                     /locus_tag="ACHLAG_000421"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFP0653729.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            16153..17577
                     /gene="degP"
                     /locus_tag="ACHLAG_000422"
     CDS             16153..17577
                     /gene="degP"
                     /locus_tag="ACHLAG_000422"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFP0653730.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            17732..18889
                     /gene="cdaR"
                     /locus_tag="ACHLAG_000423"
     CDS             17732..18889
                     /gene="cdaR"
                     /locus_tag="ACHLAG_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFP0653731.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(18978..19364)
                     /gene="yaeH"
                     /locus_tag="ACHLAG_000424"
     CDS             complement(18978..19364)
                     /gene="yaeH"
                     /locus_tag="ACHLAG_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFP0653732.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(19679..20503)
                     /gene="dapD"
                     /locus_tag="ACHLAG_000425"
     CDS             complement(19679..20503)
                     /gene="dapD"
                     /locus_tag="ACHLAG_000425"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFP0653733.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(20534..23206)
                     /gene="glnD"
                     /locus_tag="ACHLAG_000426"
     CDS             complement(20534..23206)
                     /gene="glnD"
                     /locus_tag="ACHLAG_000426"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFP0653734.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(23268..24062)
                     /gene="map"
                     /locus_tag="ACHLAG_000427"
     CDS             complement(23268..24062)
                     /gene="map"
                     /locus_tag="ACHLAG_000427"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFP0653735.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            24430..25155
                     /gene="rpsB"
                     /locus_tag="ACHLAG_000428"
     CDS             24430..25155
                     /gene="rpsB"
                     /locus_tag="ACHLAG_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFP0653736.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            25413..26264
                     /gene="tsf"
                     /locus_tag="ACHLAG_000429"
     CDS             25413..26264
                     /gene="tsf"
                     /locus_tag="ACHLAG_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFP0653737.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            26411..27136
                     /gene="pyrH"
                     /locus_tag="ACHLAG_000430"
     CDS             26411..27136
                     /gene="pyrH"
                     /locus_tag="ACHLAG_000430"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFP0653738.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            27428..27985
                     /gene="frr"
                     /locus_tag="ACHLAG_000431"
     CDS             27428..27985
                     /gene="frr"
                     /locus_tag="ACHLAG_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFP0653739.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            28077..29273
                     /gene="ispC"
                     /locus_tag="ACHLAG_000432"
     CDS             28077..29273
                     /gene="ispC"
                     /locus_tag="ACHLAG_000432"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFP0653740.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            29462..30220
                     /gene="ispU"
                     /locus_tag="ACHLAG_000433"
                     /gene_synonym="uppS"
     CDS             29462..30220
                     /gene="ispU"
                     /locus_tag="ACHLAG_000433"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFP0653741.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            30233..31090
                     /gene="cdsA"
                     /locus_tag="ACHLAG_000434"
     CDS             30233..31090
                     /gene="cdsA"
                     /locus_tag="ACHLAG_000434"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP0653742.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            31102..32454
                     /gene="rseP"
                     /locus_tag="ACHLAG_000435"
     CDS             31102..32454
                     /gene="rseP"
                     /locus_tag="ACHLAG_000435"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFP0653743.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            32484..34916
                     /gene="bamA"
                     /locus_tag="ACHLAG_000436"
     CDS             32484..34916
                     /gene="bamA"
                     /locus_tag="ACHLAG_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFP0653744.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            35038..35523
                     /gene="skp"
                     /locus_tag="ACHLAG_000437"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             35038..35523
                     /gene="skp"
                     /locus_tag="ACHLAG_000437"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFP0653745.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            35527..36552
                     /gene="lpxD"
                     /locus_tag="ACHLAG_000438"
     CDS             35527..36552
                     /gene="lpxD"
                     /locus_tag="ACHLAG_000438"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFP0653746.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            36657..37112
                     /gene="fabZ"
                     /locus_tag="ACHLAG_000439"
     CDS             36657..37112
                     /gene="fabZ"
                     /locus_tag="ACHLAG_000439"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP0653747.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            37116..37904
                     /gene="lpxA"
                     /locus_tag="ACHLAG_000440"
     CDS             37116..37904
                     /gene="lpxA"
                     /locus_tag="ACHLAG_000440"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFP0653748.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            37904..39052
                     /gene="lpxB"
                     /locus_tag="ACHLAG_000441"
     CDS             37904..39052
                     /gene="lpxB"
                     /locus_tag="ACHLAG_000441"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFP0653749.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDMVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            39049..39645
                     /gene="rnhB"
                     /locus_tag="ACHLAG_000442"
     CDS             39049..39645
                     /gene="rnhB"
                     /locus_tag="ACHLAG_000442"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFP0653750.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            39682..43164
                     /gene="dnaE"
                     /locus_tag="ACHLAG_000443"
     CDS             39682..43164
                     /gene="dnaE"
                     /locus_tag="ACHLAG_000443"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP0653751.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            43177..44136
                     /gene="accA"
                     /locus_tag="ACHLAG_000444"
     CDS             43177..44136
                     /gene="accA"
                     /locus_tag="ACHLAG_000444"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFP0653752.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            44235..46376
                     /gene="ldcC"
                     /locus_tag="ACHLAG_000445"
     CDS             44235..46376
                     /gene="ldcC"
                     /locus_tag="ACHLAG_000445"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFP0653753.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            46433..46822
                     /gene="yaeR"
                     /locus_tag="ACHLAG_000446"
     CDS             46433..46822
                     /gene="yaeR"
                     /locus_tag="ACHLAG_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP0653754.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDTLGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            46887..48185
                     /gene="tilS"
                     /locus_tag="ACHLAG_000447"
     CDS             46887..48185
                     /gene="tilS"
                     /locus_tag="ACHLAG_000447"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFP0653755.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWINSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(48234..48494)
                     /gene="rof"
                     /locus_tag="ACHLAG_000448"
     CDS             complement(48234..48494)
                     /gene="rof"
                     /locus_tag="ACHLAG_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFP0653756.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(48481..48681)
                     /gene="yaeP"
                     /locus_tag="ACHLAG_000449"
     CDS             complement(48481..48681)
                     /gene="yaeP"
                     /locus_tag="ACHLAG_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFP0653757.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            48847..49392
                     /gene="yaeQ"
                     /locus_tag="ACHLAG_000450"
     CDS             48847..49392
                     /gene="yaeQ"
                     /locus_tag="ACHLAG_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFP0653758.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     VLFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            49389..49811
                     /gene="arfB"
                     /locus_tag="ACHLAG_000451"
     CDS             49389..49811
                     /gene="arfB"
                     /locus_tag="ACHLAG_000451"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFP0653759.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGVGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            49825..50535
                     /gene="nlpE"
                     /locus_tag="ACHLAG_000452"
                     /gene_synonym="cutF"
     CDS             49825..50535
                     /gene="nlpE"
                     /locus_tag="ACHLAG_000452"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFP0653760.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDALSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPMLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGQ"
     gene            complement(50690..51513)
                     /gene="yaeF"
                     /locus_tag="ACHLAG_000453"
                     /pseudo
     CDS             complement(50690..51513)
                     /gene="yaeF"
                     /locus_tag="ACHLAG_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(51567..53285)
                     /gene="proS"
                     /locus_tag="ACHLAG_000454"
     CDS             complement(51567..53285)
                     /gene="proS"
                     /locus_tag="ACHLAG_000454"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFP0653761.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(53397..54104)
                     /gene="tsaA"
                     /locus_tag="ACHLAG_000455"
     CDS             complement(53397..54104)
                     /gene="tsaA"
                     /locus_tag="ACHLAG_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFP0653762.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(54101..54505)
                     /gene="rcsF"
                     /locus_tag="ACHLAG_000456"
     CDS             complement(54101..54505)
                     /gene="rcsF"
                     /locus_tag="ACHLAG_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFP0653763.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(54623..55438)
                     /gene="metQ"
                     /locus_tag="ACHLAG_000457"
     CDS             complement(54623..55438)
                     /gene="metQ"
                     /locus_tag="ACHLAG_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFP0653764.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(55478..56131)
                     /gene="metI"
                     /locus_tag="ACHLAG_000458"
     CDS             complement(55478..56131)
                     /gene="metI"
                     /locus_tag="ACHLAG_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFP0653765.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(56124..57155)
                     /gene="metN"
                     /locus_tag="ACHLAG_000459"
     CDS             complement(56124..57155)
                     /gene="metN"
                     /locus_tag="ACHLAG_000459"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFP0653766.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            57343..57918
                     /gene="gmhB"
                     /locus_tag="ACHLAG_000460"
     CDS             57343..57918
                     /gene="gmhB"
                     /locus_tag="ACHLAG_000460"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFP0653767.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        13769 a        14403 c        15998 g        13956 t
ORIGIN      
        1 accctctccc tcaagggaga ggggaccgat cgagcacaaa ttttgagaga tatcttcttc
       61 tgtcttgtaa cagaagaaca gaaaatcggg cttttgcgcc tgaatattgc ggagaaaaag
      121 catggccggg aatgaccgcg agccaattgg acgcaaaggg aaaccgacgc gtccggtcaa
      181 acaaaaggta agccgtcgtc gttacgaaga tgacgatgat tacgacgatt atgatgacta
      241 tgaggatgaa gaaccgatgc cgcgcaaagg taagggcaaa ggcaaagggc gtaagcctcg
      301 tggcaaacgc ggctggctat ggctactgct aaaactggct atcgtttttg ccgtgctgat
      361 cgccatttac ggcgtttatc tcgatcaaaa aattcgtagc cgtattgatg gcaaggtctg
      421 gcaactgcct gcggcagttt atggccgaat ggtcaatctt gagccagaca tgaccatcag
      481 caagaacgag atggtgaagc tgctggaggc gacccagtat cgtcaggtgt cgaaaatgac
      541 ccgtcctggc gaatttaccg tgcaggccaa cagcattgag atgattcgcc gtccgtttga
      601 tttcccggac agtaaagaag gacaggtgcg cgcgcgtctg acctttgatg gcgatcatct
      661 ggcgacgatc gtcaatatgg agaacaaccg tcagttcggt ttcttccgtc ttgatccgcg
      721 tctgatcacc atgatctctt cgccaaacgg tgagcagcgt ctgtttgtgc cgcgcagtgg
      781 tttcccggat ttgctggtgg atactttgct ggcgacagaa gaccgtcatt tttacgagca
      841 tgatggaatc agtctctact caatcggacg tgcggtgctg gcaaacctga ccgccggacg
      901 cacggtacag ggtgcgagta cgctgacgca acagctggtg aaaaacctgt tcctctccag
      961 cgagcgttct tactggcgta aagcgaacga agcttacatg gcgctgatca tggacgcgcg
     1021 ttacagcaaa gaccgtattc ttgagctgta tatgaacgag gtgtatctcg gtcagagcgg
     1081 cgacaacgaa atccgcggct tcccgctggc aagcttgtat tactttggtc gcccggtaga
     1141 agagctaagc ctcgaccagc aggcgctgtt agtcggtatg gtgaaagggg cgtccatcta
     1201 caacccgtgg cgtaacccaa aactggcgct ggagcgacgt aatctggtgc tgcgtctgct
     1261 gcaacagcaa cagattattg atcaagaact ctatgacatg ttgagtgccc gtccgctggg
     1321 ggttcagccg cgcggtgggg tgatctctcc tcagccagcc tttatgcaac tggtgcgtca
     1381 ggagctgcag gcaaaactgg gcgataaggt aaaagatctc tccggcgtga agatcttcac
     1441 tacctttgac tcggtggccc aggacgcggc agaaaaagcc gccgtggaag gcattccggc
     1501 actgaagaaa cagcgtaagt tgagcgatct tgaaactgcg attgtggtcg tcgaccgctt
     1561 tagtggtgaa gttcgtgcga tggtcggagg ttctgagccg cagtttgcgg gctacaaccg
     1621 tgcgatgcag gcgcgtcgtt cgattggttc ccttgcaaaa ccagcgactt atctgacggc
     1681 tttaagccag ccgaaaatct atcgcctgaa cacgtggatt gccgatgcgc caatcgcgct
     1741 gcgtcagccg aatggccagg tctggtcacc gcagaacgat gacagtcgtt atagcgaaag
     1801 cggcagagtg atgctggtgg atgcgttgac ccgttcgatg aacgtgccga cggtaaatct
     1861 ggggatggcg ctggggctgc ctgcagttac ggatacctgg atcaaactgg gcgtaccgaa
     1921 agatcagctg catccggttc cggcgatgct gctgggggcg ttgaacttaa cgccaatcga
     1981 agtggcgcag gcgttccaga ccatcgccag cggtggtaac cgtgcaccgc tttctgcact
     2041 gcgttcggta atcgcggaag atggcaaggt gctgtatcag agcttcccgc aggcagaacg
     2101 cgctgttccg gcgcaggcgg cgtatctgac actatggacc atgcagcagg tggtacaacg
     2161 tggtacgggt cgtcagcttg gggcgaaata cccgaacctg catctggcag ggaaaacagg
     2221 gactaccaac aataacgtag atacctggtt tgcaggtatt gacggcagca cggtgaccat
     2281 cacctgggta ggccgtgata acaaccagcc gaccaaactg tatggagcca gcggggcaat
     2341 gtcgatttat cagcgttatc tggctaacca gacgccaacg ccgctgaatc ttgttccgcc
     2401 agaagatatt gcagatatgg gcgtggacta cgacggcaac tttgtttgca gcggtggcat
     2461 gcgtgtcttg ccggtctgga ccagcgatcc gcaatcgctg tgccagcaga gcgagatgca
     2521 gcagcagccg tcaggcaatc cgtttgatca gtcttctcag ccgcagcaac agccgcaaca
     2581 gcaacctgct cagcaagagc agaaagacag cgacggtgta gccggttgga tcaaggatat
     2641 gtttggtagt aattaacatc taagcgtgaa ataccggatg gcgagttgcc atccggtaaa
     2701 ataacatccc atctaagata ttaacccttt cttttcatct ggttgtttat taacccttca
     2761 ggaacgctca gattgcgtac cgcttgcgaa cccgccagcg tttcgaatat tatcttatct
     2821 ttatgataat cattctcgtt tacgttatca ttcactttac atcagagata taccaatggc
     2881 gcgttccaaa actgctcagc caaaacactc actgcgtaaa atcgcagttg tagtagccac
     2941 agcggtaagc ggcatgtctg tttatgcaca ggcagcggtt gaaccgaaag aagacactat
     3001 caccgttacc gctgcacctg cgccgcaaga aagcgcatgg gggcctgctg caactattgc
     3061 ggcgcgacag tctgctaccg gcactaaaac cgatacgccg attcaaaaag tgccacagtc
     3121 tatttctgtt gtgaccgccg aagagatggc gctgcatcag ccgaagtcgg taaaagaagc
     3181 gcttagctac acgccgggtg tctctgttgg tacgcgtggc gcatccaaca cctatgacca
     3241 cctgatcatt cgcggctttg cggcagaagg ccaaagccag aataactatc tgaatggcct
     3301 gaagttgcag ggcaacttct ataacgatgc ggtcattgac ccgtatatgc tggaacgcgc
     3361 tgaaattatg cgtggcccgg tttccgtgct ttacggtaaa agcagtcctg gcggcctgtt
     3421 gaatatggtc agcaagcgtc cgaccaccga accgctgaaa gaagttcagt ttaaagccgg
     3481 tactgacagc ctgttccaga ctggttttga ctttagcgat tcgttggatg atgacggtgt
     3541 ttactcttat cgcctgaccg gtcttgcgcg ttctgccaat gcccagcaga aagggtcaga
     3601 agagcagcgt tatgctattg caccggcgtt cacctggcgt ccggatgata aaaccaattt
     3661 taccttcctt tcttacttcc agaacgagcc ggaaaccggt tattacggct ggttgccgaa
     3721 agagggaacc gttgagccgc tgccgaacgg taagcgtctg ccgacagact ttaatgaagg
     3781 ggcgaagaac aacacctatt ctcgtaatga gaagatggtc ggctacagct tcgatcacga
     3841 atttaacgac acctttactg tgcgtcagaa cctgcgcttt gctgaaaaca aaacctcgca
     3901 aaacagcgtt tatggttacg gcgtctgctc cgatccggcg aatgcttaca gcaaacagtg
     3961 tgcggcatta gcgccagcgg ataaaggcca ttatctggca cgtaaatacg tcgttgatga
     4021 tgagaagctg caaaacttct ccgttgatac ccagttgcag agcaagtttg ccactggcga
     4081 tatcgaccac accctgctga ccggtgtcga ctttatgcgt atgcgtaatg acatcaacgc
     4141 ttggtttggt tacgacgact ctgtgccact gctcaatctg tacaatccgg tgaataccga
     4201 tttcgacttc aatgccaaag atccggcaaa ctccggccct taccgcattc tgaataaaca
     4261 gaaacaaacg ggcgtttatg ttcaggatca ggcgcagtgg gataaagtgc tggtcaccct
     4321 gggcggtcgt tatgactggg cagatcaaga atctcttaac cgcgttgctg ggacgaccga
     4381 taaacgtgat gacaaacagt ttacctggcg tggtggtgtt aactacctgt ttgataatgg
     4441 tgtaacacct tacttcagct atagcgaatc gtttgaacct tcttcgcaag ttgggaagga
     4501 tggtaatatt ttcgcaccgt ctaaaggtaa gcagtatgaa gtcggcgtga aatatgtacc
     4561 ggaagatcgt ccgattgtag ttactggtgc cgtgtataat ctcactaaaa ccaacaacct
     4621 gatggcggac cctgagggtt ccttcttctc ggttgaaggt ggcgagatcc gcgcacgtgg
     4681 cgtagaaatc gaagcgaaag cggcgctgtc ggcgagtgtt aacgtagtcg gttcttatac
     4741 ttacaccgat gcggaataca ccaccgatac tacctataaa ggcaatacgc ctgcacaggt
     4801 gccaaaacac atggcttcgc tgtgggctga ctataccttc tttgacggtc cgctttcagg
     4861 tctgacgctg ggcaccggtg gtcgttatac tggctccagc tatggtgatc cggctaactc
     4921 ctttaaagtg ggaagttata cggtcgtgga tgcgttagtg cgttatgatc tggcgcgagt
     4981 cggcatggcg ggctccaacg tggcgctgca tgttaacaac ctgttcgatc gtgaatacgt
     5041 cgccagctgc tttaacactt atggctgctt ctggggcgca gaacgtcagg tcgttgcaac
     5101 cgcaaccttc cgtttctaat ttctcttttg gggcacggat ttccgtgccc atttcacaag
     5161 ttggctgtta tgcaggaata cacgaatcat tccgatacca cttttgcact gcgtaatatc
     5221 tcctttcgtg tgcccgggcg cacgcttttg catccgctgt cgttaacctt tcctgccggg
     5281 aaagtgaccg gtctgattgg tcacaacggt tctggtaaat ccactctgct caaaatgctt
     5341 ggccgtcatc agcctccgtc agaaggggag attcttcttg atgcccagcc gctggaaagc
     5401 tggagcagca aagcgtttgc ccgcaaagtg gcttatttgc cgcagcagct tcctccggca
     5461 gaagggatga ccgtgcgtga actggtggcg attggtcgtt acccgtggca tggcgcgctg
     5521 gggcgctttg gtgcggcaga tcgcgaaaag gtcgaggaag ctatctcgct ggttggctta
     5581 aaaccgctgg cgcatcggct ggtcgatagt ctctctggcg gcgaacgtca gcgggcgtgg
     5641 atcgccatgc tggtggcgca ggatagccgt tgtctgttgc tcgacgaacc gacttcggcg
     5701 ctggatatcg cccaccaggt tgatgtgctg gcgctggtgc accgtttaag tcaggaacgt
     5761 ggcctgacgg tcattgccgt gctgcacgat atcaatatgg cggcacgcta ctgtgattat
     5821 ctggtcgccc tgcgcggtgg tgaaatgatt gctcagggaa cgcctgcgga aattatgcgc
     5881 ggcgaaaccc tcgaaatgat ttatggcatc ccgatgggta ttttgccgca tccggcgggt
     5941 gctgcacctg tgagttttgt ttattgatga gcggcttacc tcttatttcg cgccgtcgac
     6001 tgttaacggc gatggcgctt tctccgttgt tatggcagat gaataccgcc cacgcggcgg
     6061 ctattgaccc caatcgtatt gtggcgctgg agtggttgcc ggtggaatta ctgctggcgc
     6121 tcggcatcgt gccttacggc gtggcggata ccatcaacta tcgcctgtgg gtcagcgaac
     6181 cgctattgcc ggactcagtg attgacgttg gtttgcgtac agaacctaac cttgaactgc
     6241 tgaccgaaat gaaaccgtcg tttatggtct ggtcggcagg atatggccct tcaccagaaa
     6301 tgctggcacg tattgcaccg ggtcgcggat ttaacttcag cgacggcaaa cagccgctgg
     6361 cgatggcgcg taaatcgctg acggaaatgg cagatttact taacctgcaa agcgcagcag
     6421 aaacgcattt agcgcaatat gaagacttta tccgcagcat gaaaccccgc tttgtgaagc
     6481 gtggtgcacg cccgttattg ctgacgacgc ttatcgatcc gcgccatatg ctggttttcg
     6541 gtccaaacag cttgtttcag gaaattctcg atgagtacgg catcccaaat gcctggcaag
     6601 gggaaaccaa cttctggggc agtaccgccg tcagtatcga tcgtctggcg gcgtataaag
     6661 acgttgatgt gctctgtttt gatcacgaca acagcaaaga catggatgcg ctaatggcaa
     6721 cgccgctgtg gcaggccatg ccgtttgtcc gcgccggacg ctttcagcgc gtacctgcag
     6781 tctggtttta tggtgcgacg ctctcggcaa tgcactttgt gcgcgttctg gataacgcca
     6841 tcggaggtaa agcgtgagta aacgaattgc gctttttccg gcgttattgc tggcgctgtt
     6901 ggtgattgcc gctacggcgc tcacctggat gaatttttcg caggcgctgc cgcgcagcca
     6961 gtgggcgcag gccacctggt cgccggatat tgacgtcatc gagcagatga tttttcacta
     7021 cagcttgttg ccgcgtctgg cgatttcgct gctggtgggc gcgggcctgg ggctggtggg
     7081 cgtgctgttt cagcaagtgc tgcgtaaccc gctggcggag ccgacgacgc ttggcgttgc
     7141 tacaggcgcg caactgggaa ttaccgtcac cacactttgg gcgatcccag gtgctatggc
     7201 gagccagttt gccgcgctgg tgggcgcttg tgtcgttggc ttaatcgtct ttggcgtcgc
     7261 gtgggggaaa cggctttcgc cggttacgct tattctcgcg gggctggtgg tgagccttta
     7321 ttgcggggca attaatcagc tactggttat cttccatcac gaccagctgc aaagcatgtt
     7381 cctgtggagc accggaacgc tgacgcaaac cgactggggc ggcgttgagc gtttatggcc
     7441 gcagttgctg ggcggcgtga tgttgacgtt attgctactt cgcccgttaa ccctgatggg
     7501 gcttgatgat ggcgtggcgc gcaatctcgg gctggccttg tcgctcgcgc gtctggcagc
     7561 gctgtcgctg gcgattgtca tcagtgcgct gctggtgaac gctgtgggga ttatcggctt
     7621 tatcgggttg ttcgcgccgc tgctggcaaa aatgctgggg gcgcggcgtc tgctgccacg
     7681 actgatgctg gcgtcgctga ttggggcgct gatcctctgg ctttccgatc aaatcatcct
     7741 ctggctgact cgcgtgtgga tggaagtgtc caccggttcg gtcactgcgt tgatcggtgc
     7801 gccgctgcta ctgtggctgt tgccgcgttt acgcagcatt agcgcgccgg atatgaaggt
     7861 caacgatcgt gtcgcggctg aacgccaaca tgtgctggcg tttgccctcg cgggcggcgt
     7921 gctgctgttg atggctgtgg tggtggcgct gtcgtttggt cgtgatgcgc acggctggac
     7981 gtgggcgagc ggggcgttgc ttgaggattt gatgccctgg cgctggccgc gaattatggc
     8041 ggcgctgttt gcgggcgtca tgctggcggt ggcgggctgt attattcagc ggctgaccgg
     8101 aaacccgatg gcaagcccgg aagtgctggg gattagctct ggcgcagcgt ttggcgtggt
     8161 gttgatgctg tttctggtgc cgggtaatgc ctttggctgg ctgttacctg caggcagtct
     8221 cggcgcggcg gtgacgctgt tgatcattat gatcgccgcc ggccgcggtg gattttcccc
     8281 acaccgcatg ttactggcgg ggatggcgtt aagcaccgcg ttcaccatgc ttttgatgat
     8341 gttgcaggca agtggtgacc cgcgaatggc gcaagtgctg acctggattt ccggttcgac
     8401 ctacaacgcg accgatgcgc aggtctggcg cacgggaatt gtgatggtga ttttgctggc
     8461 gattaccccg ctgtgccgcc gctggctgac cattttaccg ctgggtggtg ataccgcccg
     8521 agccgtagga atggcgctga cgccgacgcg aattgcgctg ctgctgttag cggcttgcct
     8581 gacggcgacc gcgacgatga ctattggacc gttgagtttt gttggtttaa tggcaccgca
     8641 tattgcgcgg atgatgggct ttcgacggac gatgccacac atcgtaattt cggcgctggt
     8701 gggtggttta ctgctggtgt tcgctgactg gtgtgggcgg atggtgctgt ttccattcca
     8761 gatcccggca gggctgctgt cgacctttat cggcgcgcca tattttatct atttgttgag
     8821 aaagcagatc cgttaataga atgcctcgcc cggtgggcga ggcaaaatca tcacaacttc
     8881 gcaaacaccc gtcgtgcagc atcgatggtg ttattgatat cttccatgct gtgcgccacg
     8941 gacataaagc ccgcttcaaa cgctgacggt gccaggtaaa caccttcgtc cagcatcata
     9001 tggaagaaac gcttaaagcg ttccacgtca caggccatca catcctgata gcacgtcacg
     9061 gactcggcgt cggtaaagaa aataccgaac atgccgccaa cgtggttaac gaccagcgga
     9121 attccggctt cttctgccgc ttccagcaga ccttctgcca gacgtgatgt cagctcatcc
     9181 aacgtttcgt gaacgcccgg ctgcgcgact tcattcagac aggcgaaacc cgctgccatc
     9241 gcaattgggt taccggaaag cgtacccgcc tgatagaccg gacccgtcgg ggccagcgca
     9301 tccattacat cacgacgacc accgaatgcg cctaccggca ttccaccgcc gatgattttg
     9361 ccgaggcagg ttaaatctgg cactacgccg taataatcct gtgcgccagc tagcgctacg
     9421 cggaaaccgg tcatcacttc atcgatgatc agcaacgcgc caaattcgtc gcacagcgcg
     9481 cgcagacctg gcaggaactc tggcagcggc ggaacacagt tcatattgcc tgccaccggc
     9541 tcgacgataa tacaggcaat ctcttgcggg tattgctcaa atgcggcgcg tacagaagcc
     9601 agatcattat aagtacaggt taaggtatgt ttggcgaaat ctgccggaac gcccggcgag
     9661 tttggctggc ctaacgtgag tgcgccagaa ccggctttca ccagcaggca gtcagcgtga
     9721 ccatggtaac acccttcaaa tttaataatt ttgtcgcgac cggtaaaacc acgggccagg
     9781 cggatggcgc tcatggtcgc ttcagtgccg gagttcacca tgcgcaccat atccatggtc
     9841 gggaccagtt cggtcaccag ttgcgccatt ttcacttcca tttcggttgg tgcaccaaag
     9901 cttaaaccac gctcggcggc ttcaatcacg gcattgcgga ttgccggatg gttatggccc
     9961 agcaccatcg gcccccagga accgacataa tcgatatagg ctttgccatc aacatcgtac
    10021 agataagcgc cgtccgcttt ttcgataaac agtggagtgc cgcccacgcc agtaaaggcg
    10081 cgaacagggg agttcacacc gccagggatc agctcgcgcg ctgcgctgta aagattttca
    10141 gacttactca tggagggttc ctgattcgta gaaaaagtga atggctgcta ttctatgtta
    10201 ttcataacaa gttaaatact cgtcaaacat caggctgctt gtactggtgc aatcctgatt
    10261 tcttagagta taaaagtttt gtgcatttga aacattacgc tttgcaaagg attttcatgg
    10321 aacgtgcgag taaaatgccg tcatcttatt tgtatgacca ataagtgatc attggatgaa
    10381 aactgatact ccctctttag aaacaccgca ggccgcgcgc ctgcgacgca gacaactgat
    10441 tcgccaactt cttgagcgcg ataaaacccc gttagccatt ttgtttatgg cggcagtcgt
    10501 cggcacgctt gtcgggctgg cagcggttgc ttttgacaaa ggtgtcgcct ggttgcagaa
    10561 ccaacgtatg ggggcgctgg tacatactgc tgataattat ccgcttctgt taaccgtcgc
    10621 ttttctctgt tcggcggtgc tggcgatgtt cggctacttt ttggtgcgca aatacgcgcc
    10681 ggaagcaggt ggttcgggga tcccggaaat tgaaggggcg ctggaagatc aacgtcccgt
    10741 tcgctggtgg cgtgtgttgc cggtgaaatt ctttggcggg ctggggacac tcggcggagg
    10801 catggtgcta gggcgcgaag gaccaaccgt gcagatcggc ggcaatatcg gtcgcatggt
    10861 gcttgatatt ttccgcctga aaggtgatga agctcgccat acgctgctgg caaccggtgc
    10921 tgctgcgggg ctggctgcgg cctttaacgc gccgctggcg ggtattttgt ttattatcga
    10981 agagatgcgt ccgcagtttc gctatacgtt aatttcgatt aaagcggtat ttattggcgt
    11041 cattatgtcg accattatgt accggatttt taatcatgaa gttgcgttga ttgacgtcgg
    11101 taaactttct gatgcgccgc ttaatacgct gtggctttat ctgatcctcg gtattatttt
    11161 tggcattttc ggccctattt ttaataaatg ggtgctgggg atgcaggatt tgctgcaccg
    11221 tgtgcacggc ggcaatatta ccaaatgggt actgatgggt ggtgcgattg gcggtctgtg
    11281 tggattgctg gggtttgtgg caccagcaac gtcgggcggc ggttttaacc tgattcctat
    11341 cgctaccgcg gggaatttca gcatgggaat gctggtgttt atcttcgtcg ctcgggtcat
    11401 taccacctta ctctgcttct cttccggcgc gccgggcggt atttttgccc cgatgctggc
    11461 gctgggtact gtgctgggaa ccgctttcgg aatggttgcc gttgagctgt ttccgcaata
    11521 tcacctggag gcggggacgt ttgctattgc cggaatgggg gcattactgg cggcatctat
    11581 tcgcgcgccg ttaacgggga tcattctggt tctggagatg accgataact accagctcat
    11641 tttgccaatg attattaccg gtcttggcgc aacactatta gcgcaattta ccggcgggaa
    11701 acctctatac tcggcgattc ttgcgcgcac gctggcaaaa caggaagctg agcaactggc
    11761 gcgaagcaag gccgcatcag ccagcgagaa tacttgaacg aaataccagg gtattagata
    11821 atggcgatta ttattgggtt agaatttgcc caattgccga tgtcgtttgg agcaaaatat
    11881 gagtgatgac gtagcactgc cgctggagtt taccgacgca gcagccaaca aagttaaaag
    11941 cctgatcgct gacgaagata acccgaatct gaaattacgc gtgtatatca ccggtggcgg
    12001 ttgcagcggc ttccagtatg gtttcacctt tgatgatcag gtgaacgaag gcgatatgac
    12061 catcgaaaaa cagggcgttg gcctggtggt tgatccgatg agcctgcaat atctggtcgg
    12121 cggttccgtt gactacaccg aaggactgga aggttctcgt ttcatcgtga ccaacccgaa
    12181 cgcgaaaagc acctgcggtt gcggttcttc ctttagcatc taatctgttg ttcatgtgcc
    12241 ggataaacta tccggcacat cttctctctt caacgcccat tctcatccag cgcaaacgtc
    12301 ggtagcttaa gatgccaacg aatagccgcc agccgaatca atagcgtcac gaccatgccc
    12361 atcatactgg ctgtttccag tggtacggaa aatgtgtaat aagccgtagc gtggacaata
    12421 ccgccgataa tacaggcagt tgcgtagatt tctgtacgta aaatcatggg gatctcgcgg
    12481 gccagtacat cacgaatgat cccgccgcca acgccagtaa tgacgcccat gcaaaccgcg
    12541 attaacggac cggcttccgc attaaaggct ttattcacgc caatgccgac aaacaccgcc
    12601 agaccaacgg cgtccagcac cggcaacatc cattttggta agcgtcttgg ctggcgcacc
    12661 agcacgatgg tcagcatgct ggtgaccatt gcaacgacca gatcggtggg atctttcacc
    12721 caaaataccg ggccgtgatc cagcgccatg tcgcgaattg tcccgccgcc tactgcggtt
    12781 accacgccca gtaccagaac accaaaaggg tccatacgca attttccggc taacaaaacg
    12841 ccggagatgg caaataccgc tgtgccgact atatccagcc aatagacgag cattgttcaa
    12901 tccccactga gcacctgaaa aggtcaggcg ctaatctacc tgtgaaagcg cattacagag
    12961 ctgttgtgca gcgaggataa tacgtgggct tgcacgttca aaccagtcac tcgtgagagg
    13021 aataacggga attttgagct gttcacccca gtattgtttg attttaggaa tttggtccgg
    13081 tccgcctgta atgacaatcg cctgtggcga gcgtgctaac acctgttcgc ggctaacttg
    13141 cggccaggga acccggctgt ctttaaagat gttttctccg ccacaaactt cgagtacctg
    13201 gttctgaatc gactcttttc cactggtaaa tggcggatta atgccgaatt gcagaaaaac
    13261 acgtttttta ggtttatcag catattgcgc tttcaattgc gcgtactgat cctgcaggga
    13321 ttgcgcggct tgttcggcct tgtctggttg cggactccag ggggccagtt gacgtaacgc
    13381 attggcaatt tgttcaatgc ttgtcgcatc gacccacatc acttttattc ccagcgaagc
    13441 cagctggtca acctgccgct cggcattacc tccacgccag gcaatcacca gatcgggttt
    13501 cagcgcgaca atgcgttcca gattcatccc ctgccaggtg gaaacctgct caatcttttg
    13561 cgcttgtgga ggatagtcgg aatagctgct gaccccaacc ggcgtgatcc cggcggcaaa
    13621 ggcaagttca gtgttggcgg gagaaagcgt gatgacgcgc ggcgcggcgt tgagccacag
    13681 tggcgcaaga aaagacaggg cgaccagcgc cctgaacagt gacttagcca tgtgcaagtt
    13741 tctgcaccag tgactcaacc atcaggctgg actgtttagc ggcaacagcc aggaactcat
    13801 cgaagctaag atgagactgt tgatcggcca cgtcggagat ggcgcgtacg acaacaaacg
    13861 ggacgttgaa attgtggcag acatgggcga ttgccgtcgc ttccatctct acagcaatgg
    13921 cctgtgggaa gttgtggcgg attttcgcca gaccaacaga accgttgatg aaagcgtcgc
    13981 cgctaacaat caggccacgt acagcgttaa gattcagttc ggcaatgcag gcctcagcgg
    14041 cagcgatcag tttatcgtca gctttaaagc ctgccggaca gcctggtaac tgaccgtatt
    14101 cataaccaaa tgccgtgaca tccgcgtcgt gataacgtgc ttcgtccgag acaacgatat
    14161 cgcccacttt cagcgttggt gccaggccac cggcagaacc ggtgttaata atcacatctg
    14221 gcttgcagtg ttccaacagc aaagtggcac ccagcgccgc agcgacttta ccgatgcccg
    14281 atttcagaag cgcaacctcg gttccattca gttggccggt atagatttcg caaccgccca
    14341 gactgatagt ttgacggttt tcgattttgt cacgcagcag cgtaacttct tcttccattg
    14401 caccaatgat gccgattttc atagatttac tcgcgataag cccgatttga aggcatagtt
    14461 taccatgcgc ttacggggaa gcgtatttct cacgcgggag aggacatggc acagattgat
    14521 ttccgaaaaa aaataaactg gcatcgtcgt taccgttcac cgcagggcgt taaaaccgaa
    14581 catgagatcc tgcggatctt cgagagcgat cgcgggcgta tcatcaactc tccggcaatt
    14641 cgtcgtctgc aacaaaagac gcaggttttt ccactggagc gcaatgccgc cgtgcgcacg
    14701 cgtcttaccc actcgatgga agtccagcag gtggggcgct acatcgccaa agaaatttta
    14761 agccgtctga aagagcttaa attgctggaa gcatacggcc tggatgaact gaccggacct
    14821 tttgaaagca ttgttgagat gtcatgcctg atgcacgata tcggcaatcc gccgtttggt
    14881 cattttggcg aagcagcgat aaatgactgg tttcgccaac gtttgcaccc ggaagatgcc
    14941 gaaagccagc ctctgactga cgatcgctgc agcgtggcgg cactacgttt acgggacggg
    15001 gaagaaccgc ttaacgagct gcggcgcaag attcgtcagg acttatgtca ttttgagggg
    15061 aatgcacaag gcattcgtct ggtgcataca ttgatgcgga tgaatctcac ctgggcacag
    15121 gttggcggta ttttaaaata tacccgtccg gcgtggtggc gtggcgaaac gcctgagaca
    15181 catcactatt taatgaaaaa gccgggttat tatctttctg aagaagccta tattgcccgg
    15241 ttgcgtaaag aacttaattt ggcgctttac agtcgttttc cattaacgtg gattatggaa
    15301 gctgccgacg atatctccta ttgtgtggca gaccttgaag atgcagtaga gaaaagaata
    15361 tttaccgttg agcagcttta tcatcatttg cacgaagcgt ggggccagca tgagaaaggt
    15421 tcgctctttt cgctggtggt tgaaaatgcc tgggaaaaat cacgctcaaa tagtttaagc
    15481 cgcagtacgg aagatcagtt ttttatgtat ttacgggtaa acaccctaaa taaactggta
    15541 ccctacgcgg cacaacgatt tattgataat ctgcctgcga ttttcgccgg aacgtttaat
    15601 catgcattat tggaagatgc cagcgaatgc agcgatcttc ttaagctata taaaaatgtc
    15661 gctgtaaaac atgtgtttag ccatccagat gtcgagcagc ttgaattgca gggctatcgg
    15721 gtcattagcg gattattaga gatttatcgt cctttattaa gcctgtcgtt atcagacttt
    15781 actgaactgg tagaaaaaga acgggtgaaa cgtttcccta ttgaatcgcg cttattccac
    15841 aaactctcga cgcgccatcg gctggcctat gtcgaggctg tcagtaaatt accgtcagat
    15901 tctcctgagt ttccgctatg ggaatattat taccgttgcc gcctgttgca ggattatatc
    15961 agcggtatga ccgacctcta tgcgtgggat gaataccgac gtctgatggc cgtagaacaa
    16021 taaccaggct tttgtaaaga cgaacaataa atttttacct tttgcagaaa ctttagttcg
    16081 gaacttcgcg ctataaaacg aatctgaaga acacagcaat tttgcgttat ctgttaatcg
    16141 agactgaaat acatgaaaaa aaccacatta gcactgagtg cactggctct gagtttaggt
    16201 ttggcgttat ctccgctctc tgcaacggcg gctgagactt cttcagcaac gacagcccag
    16261 cagatgccaa gccttgcacc gatgctcgaa aaggtgatgc cttcagtggt cagcattaac
    16321 gtagaaggta gcacaaccgt taatacaccg cgtatgccgc gtaatttcca gcagttcttc
    16381 ggtgatgatt ctccgttctg ccaggaaggt tctccgttcc agagctctcc gttctgccag
    16441 gggggccagg gcggtaatgg cggcggccag caacaaaaat tcatggcgct gggttccggc
    16501 gtcatcattg atgccgataa aggctatgtc gtcaccaaca accatgttgt tgataacgcg
    16561 acggtgatta aagtccagct gagcgatggt cgtaagttcg acgcgaagat ggttggcaaa
    16621 gatccgcgct ctgatatcgc gctgatccaa atccagaacc cgaaaaacct gaccgcaatt
    16681 aagatggcgg attctgatgc actgcgcgtg ggtgattaca ccgtagcgat tggtaacccg
    16741 tttggtctgg gagagacggt aacttccggg attgtctctg cgctggggcg tagcggcctg
    16801 aatgccgaaa actacgaaaa cttcatccag accgatgcag cgatcaaccg tggtaactcc
    16861 ggtggtgcgc tggttaacct gaacggcgaa ctgatcggta tcaacaccgc gatcctcgca
    16921 ccggacggcg gcaacatcgg tatcggtttt gctatcccga gcaacatggt gaaaaacctg
    16981 acctcgcaga tggtggaata cggccaggtg aaacgcggtg agctgggtat tatggggact
    17041 gagctgaatt ccgaactggc gaaagcgatg aaagttgacg cccagcgcgg tgctttcgta
    17101 agccaggttc tgcctaattc ctccgctgca aaagcgggca ttaaagcggg tgatgtgatc
    17161 acctcactga acggtaagcc aatcagcagc tttgccgcac tgcgtgctca ggtgggtact
    17221 atgccggtgg gcagcaaact gaccctgggc ttgctgcgcg acggtaagca ggtcaacgtg
    17281 aatctggaac tgcagcagag cagccagaat caggttgatt ccagctccat cttcaacggc
    17341 attgaaggtg ctgagatgag caacaaaggc aaagatcagg gcgtggtagt gaacaacgtg
    17401 aaaacgggca ctccggctgc gcagatcggc ctgaagaaag gtgatgtgat tattggcgcg
    17461 aaccagcagg cagtgaaaaa catcgctgaa ctgcgtaaag ttctcgacag caaaccgtct
    17521 gtgctggcac tgaacattca gcgcggcgac agctccatct acctgttaat gcagtaatct
    17581 ccctcaaccc cttcctgaaa acgggaaggg gttctcctta caatctgtga acttcaccac
    17641 aactccatac atcttcatca tcctttaggc atttgcacaa tgccgtacgt tacgtacttc
    17701 cttatgctaa gccgtgcata acggaggact tatggctggc tggcatcttg ataccaaaat
    17761 ggcgcaggat atcgtggcac gtaccatgcg catcatcgat accaatatca acgtaatgga
    17821 tgcccgcggg cgaattatcg gcagcggcga tcgtgagcgt attggtgaat tgcacgaagg
    17881 tgcattgctg gtactttcac agggacgagt cgtcgatatc gatgacgcgg tggcacgcca
    17941 tctgcacggt gtgcggcagg ggattaatct accgttacgg ctggaaggtg aaattgtcgg
    18001 cgtaattggc ctgacaggtg aaccagagaa tctgcgtaaa tatggcgaac tggtctgcat
    18061 gacggctgaa atgatgctgg aacagtcgcg gttgatgcac ctgttggcgc aggatagccg
    18121 tttgcgggaa gaactggtga tgaacctgat tcaggctgag gagaatactc ccgcacttac
    18181 tgaatgggcg caacggctgg ggatcgatct caatcaaccg cgagtagtgg ctattgttga
    18241 ggtcgacagc ggtcagcttg gcgtggacag cgcaatggcg gagttacaac aactgcaaaa
    18301 tgcgctgact acgcccgagc gtaataatct ggtggcgatt gtctcgctga ccgaaatggt
    18361 ggtgttgaaa ccggcgttga actcttttgg gcgctgggat gcagaagatc atcgtaagcg
    18421 agttgaacaa ctgattaccc gcatgaaaga gtatggccag ctgcgttttc gcgtttcgct
    18481 gggcaactat tttaccggtc ctggcagtat tgcccgatcc tatcgtacgg cgaaaacgac
    18541 gatggtggtg ggtaaacagc ggatgccaga aagtcgctgc tatttttatc aggatctgat
    18601 gttacctgtg ttactcgaca gtttgcgtgg cgactggcag gccaatgaac tggcgcgacc
    18661 gctggcgcgg ctgaaagcga tggacaataa tggcttgctg cgacgaacgc tggcggcgtg
    18721 gtttcgccac aatgtgcaac cgctggctac gtcaaaggcg ctgtttattc atcgtaatac
    18781 tctggagtat cggcttaatc gtatatcgga actgaccggg cttgatttgg gtaattttga
    18841 tgacaggttg ctgctgtatg tggcgttgca gctggatgaa gagcggtagg ttatgcgtta
    18901 aggtcgaggc ggcgctggct cattcccctc agcccgaagg gtagagggga atgtccgagt
    18961 taaccgtcag ttcggcatta tttacgcgtt agtttttcca gatcggcttc aatctcgctg
    19021 atcttattgg ttacgactga ctccaggtga cgtaagtcgt cgaggatctt acgcttaaga
    19081 tcaacttcgc tgcggtcacg ctggcagatt tgatcaagct catcaatgat ataccgtaga
    19141 ttcgggctga tttcctggac ttctttataa ccctgaccca caccatcagc gacgaccgtt
    19201 ttacgctgac gcggatattt aaacttaacg ctcttggcga aaaactcgcc tttgtctttc
    19261 tggaaataga ttttcagaat atcgttgttg gcttcctgcc ggaggctgta acgatcaatt
    19321 tcttcaggat tggtaatacc cagacttttc agattgtcgt acatagcgtt acctcaaaat
    19381 gagtcagtaa attgtgctta ttttagcatt tggcctggcc cgcccggctc gactgtgatc
    19441 gcagaaagcc tttttcttcc agacttctat catggcgtaa aaatcaaaaa attacctgct
    19501 ttattgtggc gatattccat gggatggatt aacacattta tcttcgtttt gcgaattaca
    19561 tgacagtccg aaacaagatg aaaaagtatt taaaaaaggg gggaagaaga atgagtagga
    19621 cgaattattc tgagccagaa agcatacggg cagcatcatg ctgcccgtat atgtactctt
    19681 aatcgatggt acgcagcagt tcgttaatgc cgactttgcc gcgagttttc gcgtcaactt
    19741 tcttaacgat aaccgcacag tagaggctgt atttgccatc tttcgacggc agattacctg
    19801 aaacaaccac agaccccgcc ggaacgcgac cgtagtggat ttcgccggtt tcacggtcgt
    19861 aaatacgggt gctctgacca atgtatacgc ccatagagat gacggaacct tcttcgacaa
    19921 tcaccccttc aaccacttcg gagcgtgcgc caatgaagca gttatcttca ataatggtcg
    19981 ggttagcctg cagcggctcc agcacgccgc cgatgcccac gccaccggaa aggtggacgt
    20041 ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt atcaaccatc gtgccttcat
    20101 caacatatgc gccgatgttg acgtaagacg gcatcagcac ggtgttacgg gcaataaatg
    20161 caccctgacg taccgccgct ggtggcacaa cgcggaagcc ttctttctga aaacgcgctt
    20221 cgtcgtagtc ggcgaatttc atcggcactt tatcgaagta gcggctttct gccccttcga
    20281 tcacctgatt atcattaata cggaaagaga gcagcaccgc ttttttcaac cactgatgcg
    20341 tcacccactg accgtcaatt ttttccgcta cacgcagtgc gccggaatcc agcagggcga
    20401 tcacctgatt taccgcttcg cgggttacgg tgtctgcatt ggctggcgtg atctcggcac
    20461 ggcgttcaaa agcggtttca ataatgttct gtaactgctg cattgttaaa ctcttttcat
    20521 atcagtaaac acatcaccct ttatcgtttg gattgagggc ctctgtcaac cgctgatgca
    20581 cttcctgctg caactcgtta ttaagtgcac gccggtcagc ggtggcaatt atgaataaat
    20641 cttctactcg ctcgccaatg gttgtaattc gggcaccatg aagcgaaatt cccagatcgg
    20701 caaaaatttt cccgactctc gccagcagtc ctggctggtc gagcgcgata agctcaagga
    20761 acgatttgcg gtcggtatgg gtcggcaaaa acgttacttc ggtttcaaca gtaaaatggc
    20821 gtaatttggc gggttggcga cggggctgcg gtggctgcca gctactttgc gtcagtactt
    20881 gctccagacc aaaccgaata acctcatgac gatctgcgga cagcgggctg ccatcgggtt
    20941 ccagcacgat aaaggtatcc atcgccatac cgtcgcgagt ggtgaaaatt tgtgcgtcgt
    21001 gaacacttaa attgcggcgg tctaattcgg cacagacggc ggcaaacaga taggggcggt
    21061 ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc ctgcgggcta agcaatacca
    21121 gcggtttgct taaatcatgc tgtaataaat ggcgggcatg ccaggccagt tgatttgggc
    21181 tatggcggac aaaatagtta gcacgacagc gtgaccaaat ttggtgcagc gcctcttcgt
    21241 cgatgttatc catgcgcagt agtgccagtg cctggagttg gtgatggcga acccgttcgc
    21301 gcatatccgg cgtgttttgc attccgcgtc gtagctgctt ttcggtggca aaatagagtt
    21361 cgcgcaacag actttgcttc cagctattcc acagcgtttc gttggtggcg caaatgtcag
    21421 ccactgtcag gcataccaga tagcgcagac gattttccgt ttgaacttct tcggcaaact
    21481 gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc tgtcaccgac atcagcaagt
    21541 gctggcgaac cagccaggca accagctgtg tttcgcgtga gttcagcccg tggagttctg
    21601 caaaatgcac tacatcctga gcaccgagaa tggagtggtc gccgccgcgt cctttggcga
    21661 tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga cggcaggcgc ggccagacgt
    21721 ccacacacaa cggatggcgc tggcgcgttt cttcactggc aaaactctcc agtttcagca
    21781 tcacgcggat agtatgttca tccaccgtgt aggcgtggaa cagatcaaac tgcatctgcc
    21841 cgacgatatg cgaccattgc ggcatatacg cgccgagcac gctatggcga tgcattggca
    21901 atagcccgcg ccgcaccgct ccggggtgac gcagaatgct caaaaacagt tttcgtgctt
    21961 ccggaatatt acacagcggt tgttgcagat ggcgacgggc atggcgtaac tggcgcagcg
    22021 tggtggagta aatgccggtg atcgcactgt tgcgcaccat ggtgtagaac atacgcaaga
    22081 tggcttccgg ctggcgcata aatagtgttt catcacgcag gtcgattagc gtaccgcgta
    22141 gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc gggaagggcg aggatggctt
    22201 catcgaacag ttgcagcagc atctggttga gttcactgac gcggcgtgta acgcggaagt
    22261 aatccttcat catccgctcg accggttcgt tgccttcacc actgtaattc agacgctgag
    22321 cgacgctaag ttggcgatcg aataacagac gattatcgta acggctgacg accagatgca
    22381 gggcaaagcg aatacgccac agtatatgca gacattcgtt taattccgcc cgctccgctg
    22441 aggttaagaa gccaaacccg accatttcat ccagcgatgt tgcgccaaaa tgacggcggg
    22501 ccacccattg cagagtgtga atatcgcgca agccgccagg gctacttttg atgtctggtt
    22561 caaggttgta gctggtgcca tggtaacgct gatggcgctg gttctgttct tcaactttcg
    22621 ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa aatatgtttt tgcagttcga
    22681 ggaagagcgc aacatcgcca attaataagc gggattcgat taaattggtg gcgacggtta
    22741 aatccgataa cccttccagc atgcactctt caagcgtgcg cacactatga ccgacttcca
    22801 gctttacatc ccagagcagc gttaacagct cgcccacttt ttgcgcctga tcgtccggga
    22861 gctttttacg gcttaaaatc agtaaatcga tgtcagaaag tggatgcagc tcgccacgac
    22921 cgtagccacc gacggcgacc aatgccaggt cggcaatctg gctgaatccc gcttcaatcc
    22981 ataatcgttg caggagctgg tcgataaact cggtgcgcgc ctcaatcaac tgttctgcag
    23041 agatcccatt gtcaaaggca tcacccagcc aacgctggaa agtatcgata tgggctttta
    23101 tcccaccgac ggttaattca tcacggggcc agacgcatgg attttgcggt tgaccgggca
    23161 gggtggggag agcggtgttt gcgtactgtt ctggaagggt attcattgtg cgccacccat
    23221 aagattaaat tatcgcatta aaaaagccgg cattcgccgg cttcatctta ttcgtcgtgc
    23281 gagattatcg ccgggatggt gtcatccttg cgtagcgtca gaatttcgca gccgttatca
    23341 gtcaccacaa tagtatgctc atattgtgca gacaagctgc gatctttggt ttttaccgtc
    23401 cagccatctt tcatggtgcg gatctctttt ttacccgcgt tgaccattgg ctcgatggtg
    23461 aacgtcatcc caggtttcag tacgacgttg gtttcacggg agtcatagtg cagcacctgc
    23521 ggttcttcat ggaagccgcg accaataccg tgtccgcaat attcacgaac gacggagaag
    23581 ccttctgctt cgacaaattt ctgaatcgcc gcaccgattt cgcgcagatt aatgcctggt
    23641 ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga tgcggcacag acgttcgccc
    23701 atgatggtcg gcttaccgac gataaacatt ttcgaggtat cgccgtggaa accatctttg
    23761 attacggtga catcaatgtt aacgatatcg ccatctttca gcagcttcgc atcgtccggg
    23821 ataccgtggc acaccacttc attaatagag atgcaaacgg atttcggata gccgtgatag
    23881 ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt aatcattaca gatgcgatcc
    23941 agctcgccgg tgctgacgcc cggtttaaca tacggttcga tcatctccag cacttcggca
    24001 gccagtcggc cagcgacgcg cattttttcg atatcttctg gggtcttgat tgagatagcc
    24061 attaattctg tccatcagcg tcggtgatac cgacaatata tatgtaagtg ccgtcaatgg
    24121 tatcacaccc gggcaaattg agaatcattc tgaatttcgc caaacgtgcc actgaacgtt
    24181 ttctataata gaaaattcaa cgtctgatgc tgtacacagc gccaacaatt attggtgtcc
    24241 atgacgtatt tgtggtataa agcgcgccgg acttccgatc catttcgtat acacagactg
    24301 gacggaagcg acaatctcac tttgtgtaac aacacacacg tatcggcaca tattccgggg
    24361 tgccctttgg ggtcggtaat atgggatacg tggaggcata accccaactt ttatatagag
    24421 gttttaatca tggcaactgt ttccatgcgc gacatgctca aggctggtgt tcacttcggt
    24481 caccagaccc gttactggaa cccgaaaatg aagccgttca tcttcggtgc gcgtaacaaa
    24541 gttcacatca tcaaccttga gaaaactgta ccgatgttca acgaagctct ggctgaactg
    24601 aacaagattg cttctcgcaa aggtaaaatc cttttcgttg gtactaaacg cgctgcaagc
    24661 gaagcggtga aagacgctgc tctgagctgc gaccagttct tcgtgaacca tcgctggctg
    24721 ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca tcaaacgtct gaaagacctg
    24781 gaaactcagt ctcaggacgg tactttcgaa aagctgacca agaaagaagc gctgatgcgc
    24841 actcgtgagc tggagaaact ggaaaacagc ctgggcggta tcaaagacat gggcggtctg
    24901 ccggacgctc tgtttgtaat cgatgctgac cacgaacaca ttgctatcaa agaagcaaac
    24961 aacctgggta ttccggtatt tgctatcgtt gataccaact ctgatccgga cggtgttgac
    25021 ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga ccctgtacct gggcgctgtt
    25081 gctgcaaccg tacgtgaagg ccgttctcag gatctggctt cccaggcgga agaaagcttc
    25141 gtagaagctg agtaataagg cttgataact ccctcaaaat agttcgagtt gcagaaaggc
    25201 ggcaagctcg agaattcccg ggagcttaca tcagtaagtg accgggatga gcgagcgaag
    25261 acaacgcatc tgcggcgcga aatatgaagg gggagagccc ttatagacca ggtagtacac
    25321 gtttggttag ggggcctgca tatggccccc tttttcactt ttatatctgt gcggtttaat
    25381 gccgggcaga tcacatctcc gaggatttta gaatggctga aattaccgca tccctggtaa
    25441 aagagctgcg tgagcgtact ggcgcaggca tgatggattg caaaaaagca ctgactgaag
    25501 ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa gtccggtgct attaaagcag
    25561 cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa aaccaaaatc gacggcaact
    25621 acggcatcat tctggaagtt aactgccaga ctgacttcgt tgcaaaagac gctggtttcc
    25681 aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg caaaatcact gacgttgaag
    25741 ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt agcgaaaatt ggtgagaaca
    25801 tcaacattcg ccgcgttgct gcgctggaag gcgacgttct gggttcttat cagcacggtg
    25861 cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga agagctggtt aaacacatcg
    25921 ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc agaagacgta tccgctgaag
    25981 tggtagaaaa agaataccag gtacagctgg atatcgcgat gcagtctggt aagccgaaag
    26041 aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt caccggcgaa gtttctctga
    26101 ccggtcagcc gttcgttatg gaaccaagca aaactgttgg tcagctgctg aaagagcata
    26161 acgctgaagt gactggcttc atccgcttcg aagtgggtga aggcatcgag aaagttgaga
    26221 ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc ttaattatcg aaaaggagcc
    26281 gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc agctaaccct tgtggggctg
    26341 cgctgaaaag cgacgtacaa tgtcgctagt attaattcat ttcaatcgtt gacagtctca
    26401 ggaaagaaac atggctacca atgcaaaacc cgtctataaa cgcattctgc ttaagttgag
    26461 tggcgaagct ctgcagggca ctgaaggctt cggtattgat gcaagcatac tggatcgtat
    26521 ggctcaggaa atcaaagaac tggttgaact gggtattcag gttggtgtgg tgattggtgg
    26581 gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt atgaaccgcg ttgtgggcga
    26641 ccacatgggg atgctggcga ccgtaatgaa cggcctggca atgcgtgatg cactgcaccg
    26701 cgcctatgtg aacgctcgtc tgatgtccgc tattccattg aatggcgtgt gcgacagcta
    26761 cagctgggca gaagctatca gcctgttgcg caacaaccgt gtggtgatcc tctccgccgg
    26821 tacaggtaac ccgttcttta ccaccgactc agcagcttgc ctgcgtggta tcgaaattga
    26881 agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg tttactgctg atccggcgaa
    26941 agatccaact gcaaccatgt acgagcaact gacttacagc gaagtgctgg aaaaagagct
    27001 gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac cataaattac cgattcgtgt
    27061 tttcaacatg aacaaaccgg gtgcgctgcg ccgtgtggta atgggtgaaa aagaagggac
    27121 tttaatcacg gaataattcc cgtgatggat aaataagggt aagattccgc ataagtatcg
    27181 cgggggcgta agtctggtta taaggcgtta ttgttgcagg cagtttggtc acggccagcg
    27241 cgcagcaacc ggtgcgtaca caagtacgtg aggatggcga gcactacccg gggccaaaat
    27301 ggcaaataaa atagcctaat aatccagacg attacccgta atatgtttaa tcagggctat
    27361 acttagcaca cttccactgt gtgtgactgt ctggtctgac tgagacaagt tttcaaggat
    27421 tcgtaacgtg attagcgata tcagaaaaga tgctgaagta cgcatggaca aatgcgtaga
    27481 agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt gcttctccca gcctgctgga
    27541 tggcattgtc gtggaatatt acggcacgcc gacgccgctg cgtcagctgg caagcgtaac
    27601 ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat cgttcaatgt ctccggccgt
    27661 tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg aactctgcgg gtagcgacat
    27721 ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa gatctgacca aaatcgttcg
    27781 tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg cgtcgtgacg cgaacgacaa
    27841 agtgaaagca ctgttgaaag ataaagagat cagcgaagac gacgatcgcc gttctcagga
    27901 cgatgtacag aaactgactg atgctgcaat caagaaaatt gaagcggcgc tggcagacaa
    27961 agaagcagaa ctgatgcagt tctgatttct tgaacgacaa aaacgccgct cagtagatcc
    28021 ttgcggatcg gctggcggcg ttttgctttt tattctgtct caactctgga tgtttcatga
    28081 agcaactcac cattctgggc tcgaccggct cgattggttg cagcacgctg gacgtggtgc
    28141 gccataatcc cgaacacttc cgcgtagttg cgctggtggc aggcaaaaat gtcactcgca
    28201 tggtagaaca gtgcctggaa ttctctcccc gctatgccgt aatggacgat gaagcgagtg
    28261 cgaaacttct taaaacgatg ctacagcaac agggtagccg caccgaagtc ttaagtgggc
    28321 aacaagccgc ttgcgatatg gcagcgcttg aggatgttga tcaggtgatg gcagccattg
    28381 ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg cgcgggtaaa accattttgc
    28441 tggccaataa agaatcactg gttacctgcg gacgtctgtt tatggacgcc gtaaagcaga
    28501 gcaaagcgca attgttaccg gtcgatagcg aacataacgc catttttcag agtttaccgc
    28561 aacctatcca gcataatctg ggatacgctg accttgagca aaatggcgtg gtgtccattt
    28621 tacttaccgg gtctggtggc cctttccgtg agacgccatt gcgcgatttg gcaacaatga
    28681 cgccggatca agcctgccgt catccgaact ggtcgatggg gcgtaaaatt tctgtcgatt
    28741 cggctaccat gatgaacaaa ggtctggaat acattgaagc gcgttggctg tttaacgcca
    28801 gcgccagcca gatggaagtg ctgattcacc cgcagtcagt gattcactca atggtgcgct
    28861 atcaggacgg cagtgttctg gcgcagctgg gggaaccgga tatgcgtacg ccaattgccc
    28921 acaccatggc atggccgaat cgcgtgaact ctggcgtgaa gccgctcgat ttttgcaaac
    28981 taagtgcgtt gacatttgcc gcaccggatt atgatcgtta tccatgcctg aaactggcga
    29041 tggaggcgtt cgaacaaggt caggcagcga cgacagcatt gaatgccgca aacgaaatca
    29101 ccgttgctgc ttttcttgcg caacaaatcc gctttacgga tatcgctgcg ttgaatttat
    29161 ccgtactgga aaaaatggat atgcgcgaac cacaatgtgt ggacgatgtg ttatctgttg
    29221 atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg tctcgcaagc tgaggataat
    29281 ccggctacag agagtcgcgc tatttgttag cgtagggctt cagtgatata gtctgcgcca
    29341 tctgatcgta agtagttggc tttataaggt caggtatgcc gtggttttac acggcttttt
    29401 tttgtatagg cttcagtatt cctgagtacc gtaaaccctg tcagggaata aaaaacgcgt
    29461 gatgttgtct gctactcaac cacttagcga aaaattgcca gcgcatggct gccgtcatgt
    29521 tgcgatcatt atggacggca atggtcgctg ggcaaaaaag caagggaaga ttcgtgcctt
    29581 tgggcataaa gccggggcaa aatccgtccg ccgggctgtc tcttttgcgg ccaacaacgg
    29641 tattgaggcg ttaacgctgt atgcctttag tagtgaaaac tggaaccgac cagcgcagga
    29701 agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat agcgaagtaa aaagtctgca
    29761 ccgacataac gtgcgtctgc gtattattgg cgataccagt cgctttaact cgcgtttgca
    29821 agaacgtatt cgtaaatctg aagcgctaac agccgggaat accggtctga cgctgaatat
    29881 tgcggcgaac tacggtggac gttgggatat agtccaggga gtcaggcaac tggctgaaaa
    29941 ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa gagatgctaa accagcatgt
    30001 ctgtatgcat gaactggccc ctgtagattt agtaattagg actggggggg agcatcgcat
    30061 tagtaacttt ttgctttggc aaattgccta tgccgaactt tactttacag atgttctctg
    30121 gcccgatttc gatgaacaag actttgaagg ggcgttaaat gcctttgcta atcgagagcg
    30181 tcgtttcggc ggcaccgagc ccggtgatga aacagcctga tgggggtcgc ttttgctgaa
    30241 gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc atcgcggcgt tgtttctgtt
    30301 gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc atgctggcag cgtgggaatg
    30361 gggacagctt agcggtttta ccactcgttc gcagcgagta tggttggcgg tgttatgcgg
    30421 gttattgttg gcgctgatgc tttttctgtt gccggaatat caccgaaata ttcatcaacc
    30481 gctggttgaa atctcacttt gggcttcgct gggttggtgg attgtcgcgc tattgctggt
    30541 gctgttttac ccaggttccg cagcaatctg gcgtaactct aaaacattgc gccttatttt
    30601 tggcgtgcta accattgttc ccttcttctg gggcatgctg gcgttacggg cctggcacta
    30661 tgacgagaat cattacagtg gcgcaatatg gctgctctat gtcatgatcc tggtatgggg
    30721 cgctgactcc ggcgcatata tgtttggcaa attgtttggt aaacataagc tggcaccgaa
    30781 ggtttctccg ggtaaaacct ggcaaggctt tataggtgga ctagcgaccg cagcggtaat
    30841 ctcatggggt tatggcatgt gggcgaatct cgacgtcgct cccgtcacct tactcatttg
    30901 ctctattgtc gcagcgttag cctcagtgct cggcgatctg accgagagta tgtttaagcg
    30961 tgaagcagga attaaggaca gcggtcattt aattccagga cacggtggta ttttagatcg
    31021 tattgatagc ctgacggctg cggtaccggt ctttgcttgc ttgttgttac tggtattcag
    31081 gacgctttaa cggaaggtaa tatgctgagt tttctctggg atttggcttc gttcatcgtt
    31141 gcactgggtg tacttatcac cgtgcatgaa tttggtcatt tctgggttgc ccggcgttgt
    31201 ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg cgctctggcg gcgaactgat
    31261 aagctcggca ccgaatatgt tatcgccctg atcccgctgg gcggttatgt caaaatgctg
    31321 gatgagcgcg cagaaccggt cgttccggaa ctccgccacc atgccttcaa taataaatct
    31381 gttggccaac gagcggcgat tattgccgca ggtccggttg caaacttcat ttttgctatc
    31441 tttgcctact ggctggtttt tattattggt gtgcctggcg tacgtccggt ggttggtgaa
    31501 atagcagcca attcgatagc tgcggaagca caaattgcac caggtacgga actaaaagcc
    31561 gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt tgcagttggt cgataaaatt
    31621 ggcgatgaaa gcaccaccat tacagtagcg ccatttggca gcgatcaacg gcgggatgta
    31681 aagctcgatt tacgtcactg ggcgtttgag cctgataaag aagatccggt atcttcgctg
    31741 gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg aaaatgtgca gccaaactcg
    31801 gcggcaagca aggcaggttt gcaagcaggc gacaggatcg ttaaagtcga tggtcagccc
    31861 ttaacgcagt gggtgacctt tgtgatgctt gtccgggata acccgggtaa atccttagcg
    31921 ttagaaatcg aaaggcaggg aagtcccttg tctttgacat taatcccgga gagtaaaccg
    31981 ggtaatggta aagcgattgg ttttgtcggt attgagccga aagtcattcc tttgccagat
    32041 gagtataaag ttgtacgcca gtatgggccg ttcaacgcca tcgtcgaagc cacggacaaa
    32101 acgtggcagc tgatgaagct gacggtcagt atgctgggaa aattgatcac cggtgatgtg
    32161 aaactgaaca acctcagtgg gccgatctct atcgccaagg gggctgggat gacagcggaa
    32221 ctcggggtag tttattacct gccgtttctt gcgcttatta gcgtgaactt agggataatt
    32281 aacctgtttc cgttgcccgt acttgacggg gggcatctgc tgttccttgc gatcgaaaag
    32341 atcaagggcg gaccggtatc cgagcgggtt caagactttt gttatcgcat tggctcgatt
    32401 ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt tctctcggtt atgagagtta
    32461 gttaggaaga acgcataata acgatggcga tgaaaaagtt gctcatagcg tcgctgctgt
    32521 ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt gaaagatatt catttcgaag
    32581 gccttcagcg tgtcgccgtt ggtgcggccc tcctcagtat gccggtgcgc acaggcgaca
    32641 cggttaatga tgaagatatc agtaatacca ttcgcgctct gtttgctacc ggcaactttg
    32701 aggatgttcg cgtccttcgt gatggtgata cccttctggt tcaggtaaaa gaacgtccga
    32761 ccattgccag cattactttc tccggtaaca aatcggtgaa agatgacatg ctgaagcaaa
    32821 acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga tcgcaccacc attgccgata
    32881 tcgagaaagg tctggaagac ttctactaca gcgtcggtaa atatagcgcc agcgttaaag
    32941 ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa actggtgttc caggaaggtg
    33001 tgtcagctga aatccagcaa attaacattg ttggtaacca tgctttcacc accgacgaac
    33061 tgatctctca tttccaactg cgtgacgaag tgccgtggtg gaacgtggta ggcgatcgta
    33121 aataccagaa acagaaactg gcgggcgacc ttgaaaccct gcgcagctac tatctggatc
    33181 gcggttatgc ccgtttcaac atcgactcta cccaggtcag tctgacgcca gataaaaaag
    33241 gtatttacgt cacggtgaac attaccgaag gcgatcagta caagctttct ggcgttgaag
    33301 tgagcggcaa ccttgccggg cactccgctg aaattgagca gctgactaag atcgagccgg
    33361 gcgagctgta taacggcacc aaagtgacca agatggaaga tgacatcaaa aagcttctcg
    33421 gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc tgaaattaac gatgccgaca
    33481 aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg tttctacgtg cgtaagatcc
    33541 gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg tcgcgaaatg cgtcagatgg
    33601 aaggtgcatg gctgggaagc gatctggtcg atcagggtaa ggagcgtctg aatcgtctgg
    33661 gcttctttga aactgtcgat accgataccc aacgtgttcc gggtagcccg gaccaggtag
    33721 atgtcgtcta caaggtaaaa gagcgcaaca ccggtagctt caactttggt attggttacg
    33781 gtactgaaag tggcgtgagc ttccaggctg gtgtgcagca ggataactgg ttaggtacag
    33841 gttatgctgt tggtatcaac gggaccaaaa acgattacca gacctatgct gaactgtcgg
    33901 taaccaaccc gtacttcact gtagatggtg taagcctcgg tggtcgtctc ttctataatg
    33961 acttccaggc agatgacgcc gacctgtccg actataccaa caagagttat ggtacagacg
    34021 tgacgttggg cttcccgatc aacgaatata actcgctgcg tgcaggtctg ggttatgtac
    34081 ataactccct gtccaacatg cagcctcagg ttgcgatgtg gcgttatctg tactctatgg
    34141 gtgaacatcc gagcacctct gatcaggata atagcttcaa aacggacgac ttcacgttca
    34201 actatggttg gacctataac aagcttgacc gtggttactt cccaacagat ggttcacgtg
    34261 tcaacctgac cggtaaagtg accattcctg gatcggataa cgaatactac aaagtgacgt
    34321 tagacacggc gacttatgtg ccgatcgatg acgatcacaa atgggttgtt ctggggcgta
    34381 cccgctgggg ttatggtgat ggtttaggcg gcaaagagat gccgttctac gagaacttct
    34441 atgccggtgg ttccagcacc gtgcgtggct tccagtccaa tacaattggt ccgaaagcag
    34501 tttacttccc gcatcaggcc agtaattatg atccggacta tgattacgaa tgtgcgactc
    34561 aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt aggcggtaac gccatggcgg
    34621 ttgccagcct cgagttcatc accccgacgc cgtttattag cgataagtat gctaactcgg
    34681 ttcgtacttc cttcttctgg gatatgggta ccgtttggga tacaaactgg gattccagcc
    34741 aatattctgg atatccggac tatagtgatc caagcaatat ccgtatgtct gcgggtatcg
    34801 cattacaatg gatgtcccca ttggggccgt tggtgttctc ctacgcccag ccgttcaaaa
    34861 agtacgatgg agacaaggca gaacagttcc agtttaacat cggtaaaacc tggtaagtgt
    34921 tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt aggcgatcaa tataagatcg
    34981 ccgggccacg caaagaactg caccctccgg tgcaaatggg atggtaagga gtttattgtg
    35041 aaaaagtggt tattagctgc aggtctcggt ttagcactgg caacttctgc tcaggcggct
    35101 gacaaaattg caatcgtcaa catgggcagc ctgttccagc aggtagcgca gaaaaccggt
    35161 gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca gcgaactgca gcgtatggaa
    35221 accgatctgc aggctaaaat gaaaaagctg cagtccatga aagcgggcag cgatcgcact
    35281 aagctggaaa aagacgtgat ggctcagcgc cagacttttg ctcagaaagc gcaggctttt
    35341 gagcaggatc gtgcacgtcg ttccaacgaa gaacgcggca aactggttac tcgtatccag
    35401 actgctgtga aatccgttgc caacagccag gatatcgatc tggttgttga tgcaaacgcc
    35461 gttgcttaca acagcagcga tgtaaaagac atcactgccg acgtactgaa acaggttaaa
    35521 taagtaatgc cttcaattcg actggctgat ttagcgcagc agttggatgc agaactacac
    35581 ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc aatctgcaca aacaggtcac
    35641 attacgttca tggttaaccc aaaataccgt gagcatttag gcttgtgcca ggcgtccgcg
    35701 gttgtcatga cccaggacga tcttcctttc gcgaaaagtg ccgcgctggt agtgaagaat
    35761 ccctacctga cttacgcgcg catggcgcaa attttagata ccacgccgca gcccgcgcag
    35821 aacattgcac ccagtgcggt gatcgacgcg acggcgaagc tgggtaacaa cgtatcgatt
    35881 ggcgctaacg cggtgattga gtccggcgtt gaactgggcg ataacgtgat tatcggtgcc
    35941 ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt cgcgtctctg ggcgaacgta
    36001 accatttacc atgagatcca gatcggtcag aattgcctga tccagtccgg aacagtggta
    36061 ggcgcagacg gctttggtta tgccaacgat cgtggtaact gggtgaagat cccacagatt
    36121 ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct gcacaaccat cgatcgcggc
    36181 gcgctggatg acactgttat tggcaatggc gtgatcattg ataaccagtg ccagattgca
    36241 cataacgtcg tgattggcga caatacggcg gttgccggtg gcgtcattat ggcgggcagc
    36301 ctgaaaattg gtcgttactg catgatcggc ggagccagcg taatcaacgg gcatatggaa
    36361 atatgcgaca aagtgacggt tacgggcatg ggtatggtga tgcgtcccat cactgaacca
    36421 ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag tctggcgcaa aaccgctgca
    36481 ctggtgatga acattgatga catgagcaag cgtctgaaat cgcttgagcg caaggttaat
    36541 caacaagact aacgttccat cttttgttcg ccaaacttta cggcctgtct cattcttacg
    36601 attgcggcag gccgtgttat tattgtcgtt tcttatattt tgacaggaag agtatcttga
    36661 ctactaacac tcatactctg cagattgaag agattttaga acttctgccg caccgtttcc
    36721 cgttcttact ggtggatcgc gtgctggatt ttgaagaagg tcgttttctg cgcgcagtaa
    36781 aaaatgtctc tgtcaatgag ccattcttcc agggccattt ccctggaaaa ccgattttcc
    36841 cgggtgtgct gattctggaa gcaatggcac aggcaacagg tattctggcg tttaaaagcg
    36901 taggaaaact ggaaccgggt gagctgtact acttcgctgg tattgacgaa gcgcgcttca
    36961 agcgcccggt cgtgcctggc gatcaaatga tcatggaagt cactttcgaa aaaacgcgcc
    37021 gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg taaagtagtt tgcgaagcaa
    37081 cgatgatgtg tgctcgtagc cgggaggcct gataagtgat tgataaatcc gcctttgtgc
    37141 atccaaccgc cattgtggaa gagggcgcgt caattggcgc gaacgcacac attggtcctt
    37201 tttgtatcgt tggaccccat gtcgaaattg gtgagggtac cgtactgaaa tctcacgttg
    37261 tcgtgaatgg tcatactaaa attggccgcg ataatgagat ttatcagttc gcctccatcg
    37321 gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac ccgtgtggaa atcggcgatc
    37381 gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac agtccagggc ggtggattga
    37441 cgaaggtggg cagcgacaac ttactgatga tcaacgcgca cattgcgcac gattgtacgg
    37501 taggtaaccg ctgtattctc gccaacaacg caacgctggc gggtcacgta tcggttgacg
    37561 acttcgcgat catcggcggc atgaccgcag tccatcagtt ctgcatcatt ggtgcgcacg
    37621 tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc tccttatgtc attgcgcagg
    37681 gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct gaagcgccgc ggattcagcc
    37741 gtgaggcgat taccgctatc cgcaatgcgt ataagctgat ttatcgtagc ggtaaaacgc
    37801 tcgatgaagt gaaaccggaa attgctgaac tggcggaaac atatccggaa gtgaaagcct
    37861 ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg ttaatgactg aacagcgtcc
    37921 attaacgatt gccctggtcg ccggagaaac ctccggcgat atcctggggg ccggtttaat
    37981 ccgcgctctg aaagaacgtg tgcccaacgc ccgctttgtt ggtgttgccg ggccacgaat
    38041 gcaggctgaa ggctgcgaag cctggtacga aatggaagaa ctggcggtga tgggcattgt
    38101 tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt gccgatctga caaagcgttt
    38161 tggcgaactg aagccagata tttttgttgg tattgatgcg cctgacttca atattactct
    38221 tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat tacgtcagtc catccgtctg
    38281 ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc accgatatgg tgctcgcatt
    38341 tctgcctttc gaaaaagcgt tttatgacaa atacaatgta ccgtgccgct ttatcggtca
    38401 taccatggct gatgccatgc cgttagatcc agataaaaat gccgcccgtg atgtgctggg
    38461 gatccctcac gatgcccact gcctggcgtt gctaccaggg agccgtggtg cagaagttga
    38521 aatgcttagt gccgatttcc tgaaaacggc ccagcttttg cgccagacat atccggatct
    38581 cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag cagtttgaac gcatcaaagc
    38641 tgaagtcgcg ccagaccttt cagttcattt gctggatggg atgggccgtg aggcgatggt
    38701 cgccagcgat gcggcgctac tggcgtcggg tacggcagcc ctggagtgta tgctggcgaa
    38761 atgcccgatg gtggtgggat atcgcatgaa gccttttacc ttctggttgg cgaagcggct
    38821 ggtgaaaact gattatgtct cgctgccaaa tctgctggcg ggtagagagt tagtcaaaga
    38881 attattgcag gaagagtgtg agccgcaaaa actggctgcg gcgctgttac cgctgttggc
    38941 gaacgggaaa accagccacg cgatgcacga taccttccgt gaactgcatc agcagatccg
    39001 ctgcaatgcc gatgagcagg cggcacaagc cgttctggag ttagcacaat gatcgaattt
    39061 gtttatccgc acacgcagct ggttgcgggt gtggatgaag tcggacgcgg gccgttagtt
    39121 ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc gcccgattgc cgggctgaat
    39181 gattccaaaa agctgagcga aaaacgccgt ctggcgctct atgaagagat caaagagaaa
    39241 gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa tcgacgagct gaacattctt
    39301 catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc tgcatattgc gccggaatat
    39361 gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc ctgcgatggc tgtggtgaaa
    39421 ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc tggcgaaagt gacgcgtgac
    39481 gccgaaatgg cggcgctgga tattgttttc ccgcaatatg gttttgccca acacaaaggg
    39541 tacccaaccg cttttcatct ggaaaaactg gctgaacacg gcgcgaccga acaccatcgg
    39601 cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt cctgattctt gtgtcgagat
    39661 taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg tacacctgcg ggtgcacagc
    39721 gactactcga tgatcgatgg cctggccaaa accgcaccgt tggtaaaaaa ggcggcggcg
    39781 ttgggtatgc cagcactggc gatcaccgat ttcaccaacc tttgtggtct ggtgaagttc
    39841 tacggagcgg gacatggcgc agggattaag cctatcgtcg gggcagattt taacgtccag
    39901 tgcgacctgc tgggtgatga gttaacccac ctgacggtac tggcggcgaa caataccggc
    39961 tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc gcgggtacgg tgccgccggg
    40021 ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag ggttgatcct tctttccggc
    40081 ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta acagcgcgct ggtagatgag
    40141 tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt ttctcgagct gatccgcacc
    40201 ggcaggccgg atgaagaaag ctatctgcac gcggcggtgg aactggcgga agcgcgcggt
    40261 ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca gcagcgactt tgacgcacac
    40321 gaaatccgcg tcgcgatcca cgacggcttt accctcgacg atcctaaacg cccgcgtaac
    40381 tattcgccgc agcaatatat gcgtagcgaa gaggagatgt gtgagctgtt tgccgacatc
    40441 cccgaagccc ttgccaacac cgttgagatc gccaaacgct gtaacgtaac cgtgcgtctt
    40501 ggtgaatact tcctgccgca gttcccgacc ggggacatga gcaccgaaga ttatctggtc
    40561 aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt tattccctga cgaggaagaa
    40621 cgtgttaagc gccgcccgga atatgacgaa cgtctggaga ctgaacttca ggttatcaac
    40681 cagatgggct tcccgggcta cttcctcatc gttatggaat ttatccagtg gtcgaaagat
    40741 aacggcgtac cggtagggcc aggccgtggc tccggtgcgg gttcactggt ggcctacgcg
    40801 ctgaaaatca ccgacctcga tccgctggaa tttgacctgc tgttcgaacg tttccttaac
    40861 ccggaacgtg tctccatgcc tgacttcgac gttgacttct gtatggagaa acgcgatcag
    40921 gttatcgagc acgtagcgga catgtacggt cgtgatgcgg tatcgcagat catcaccttc
    40981 ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc gcgtgctggg gcatccgtac
    41041 ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc cggggatgac gctggcgaaa
    41101 gcgtttgaag ccgagccgca gctgccggaa atctacgaag cggatgaaga agttaaggcg
    41161 ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta acgccggtaa gcacgccggt
    41221 ggggtggtta tcgcgccgac caaaattacc gattttgcgc cgctttactg cgatgaagag
    41281 ggcaaacatc cggtcaccca gtttgataaa agcgacgttg aatacgccgg actggtgaag
    41341 ttcgacttcc ttggtttgcg tacgctcacc atcatcaact gggcgctgga gatgatcaac
    41401 aagcggcggg cgaagaatgg cgagccgccg ctggatatcg ccgcgatccc gctggatgac
    41461 aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg cggtattcca gcttgaatcg
    41521 cgcggcatga aggacctgat caagcgtctg caacctgact gcttcgaaga tatgatcgca
    41581 ctggtggcgc tgttccgccc aggtccgttg caatcaggga tggtggataa ctttatcgac
    41641 cgtaaacatg gtcgcgaaga aatctcctat ccggacgtac agtggcagca tgaaagcctg
    41701 aaaccggtac tggagccaac ctacggcatc atcctgtatc aggaacaggt tatgcagatt
    41761 gcgcaggtgc tttctggtta taccctcggt ggcgcggata tgctgcgtcg tgcgatgggt
    41821 aagaaaaagc cggaagagat ggctaagcag cgttctgtat ttgctgaagg tgcagaaaag
    41881 aacggaatca acgccgaact ggcgatgaaa atcttcgacc tggtggagaa attcgcgggt
    41941 tacggattta acaaatcgca ctctgcggcc tatgctttgg tgtcatatca aacgttatgg
    42001 ctgaaagcgc actatcctgc ggagtttatg gcggcggtaa tgaccgccga tatggacaac
    42061 accgagaagg tggtgggcct ggtggatgag tgctggcgga tggggctgaa aatcctgcca
    42121 ccagatataa actccggtct ttaccatttc cacgtcaacg acgacggcga aatcgtgtat
    42181 ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg aggccatcat cgaagcccgt
    42241 aataaaggcg gctacttccg cgaactgttt gatctttgcg cccgtaccga caccaaaaag
    42301 ttaaaccggc gggtgctgga aaaactgatc atgtccgggg cgtttgaccg tcttggacca
    42361 caccgcgcgg cactgatgaa ctcgctgggc gatgcgttaa aagcggcaga tcaacacgcg
    42421 aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc tggccgaaga gccggaacaa
    42481 attgaacaat cctacgccag ctgccaaccg tggccggagc aggtggtatt agatggggaa
    42541 cgtgaaacgt taggcctgta cctgaccgga caccctatca accagtattt aaaagagatt
    42601 gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc cgacagaacg tggtaaagtc
    42661 atcacggctg cggggctcgt tgttgccgcg cgggttatgg tcaccaagcg cggcaatcgt
    42721 atcggtatct gcacgctgga tgaccgttcc gggcggctgg aagtgatgtt gtttactgac
    42781 gccctggata aataccagca attgctggaa aaagaccgca tacttatcgt cagcggacag
    42841 gtcagctttg atgacttcag cggtgggctt aaaatgaccg ctcgcgaagt gatggatatt
    42901 gacgaagccc gggaaaaata tgctcgcggg cttgctatct cgctgacgga caggcaaatt
    42961 gatgaccagc ttttaaaccg actccgtcag tctctggaac cccaccgctc tgggacaatt
    43021 ccagtacatc tctactatca gagggcggat gcacgcgcgc ggttgcgttt tggcgcgacg
    43081 tggcgtgtct ctccgagcga tcgtttatta aacgatctcc gtggcctcat tggttcggag
    43141 caggtggaac tggagtttga ctaatacagg aatactatga gtctgaattt ccttgatttt
    43201 gaacagccga ttgcagagct ggaagcgaaa atcgattctc tgactgcggt tagccgtcag
    43261 gatgagaaac tggatattaa catcgatgaa gaagtgcatc gtctgcgtga aaaaagcgta
    43321 gaactgacac gtaaaatctt cgccgatctc ggtgcatggc agattgcgca actggcacgc
    43381 catccacagc gtccttatac cctggattac gttcgcctgg catttgatga atttgacgaa
    43441 ctggctggcg accgcgcgta tgcagacgat aaagctatcg tcggtggtat cgcccgtctt
    43501 gacggtcgtc cggtgatgat cattggtcat caaaaaggtc gtgaaaccaa agaaaaaatt
    43561 cgccgtaact ttggtatgcc agcgccagaa ggttaccgca aagcactgcg tctgatgcaa
    43621 atggctgaac gctttaagat gcctatcatc acctttatcg acaccccggg ggcttatcct
    43681 ggcgtgggcg cagaagagcg tggtcagtct gaagccattg cacgcaacct gcgtgaaatg
    43741 tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg aaggtggttc tggcggtgcg
    43801 ctggcgattg gcgtgggcga taaagtgaat atgctgcaat acagcaccta ttccgttatc
    43861 tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg acaaagcgcc gctggcggct
    43921 gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga aactgatcga ctccatcatc
    43981 ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga tggcggcatc gttgaaagcg
    44041 caactgctgg cggatctggc cgatctcgat gtgttaagca ctgaagattt aaaaaatcgt
    44101 cgttatcagc gcctgatgag ctacggttac gcgtaattcg caaaagttct gaaaaagggt
    44161 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
    44221 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
    44281 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
    44341 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgcggcgtga
    44401 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
    44461 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
    44521 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
    44581 gtatgcgtca gtacaccgac gaatatcttg ataacattac accgccgttc acgaaagcct
    44641 tgtttaccta cgttaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
    44701 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
    44761 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
    44821 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
    44881 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
    44941 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
    45001 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
    45061 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
    45121 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
    45181 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
    45241 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
    45301 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
    45361 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
    45421 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagacggcg gcggcgatgc
    45481 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
    45541 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
    45601 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
    45661 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
    45721 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcagcg ctggtggcaa
    45781 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
    45841 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
    45901 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
    45961 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
    46021 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
    46081 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
    46141 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
    46201 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
    46261 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
    46321 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
    46381 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
    46441 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
    46501 ctactgcgat actcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
    46561 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
    46621 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
    46681 tgatgacatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
    46741 ccgtgtcgat ccatacacgc aaaaacgctt caccttcttt aacgatccgg acgggctgcc
    46801 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
    46861 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
    46921 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
    46981 tggtgcagtg gcggacggaa aatccgggcg tcactctgcg cgctattcat gtgcatcacg
    47041 gtttaagtgc caatgccgat gcctgggtta cgcattgcga aaacgtctgc caacagtggc
    47101 aggtgccgct ggtggtcgaa cgcgtacaac ttgcgcaaga ggggctgggg attgaggccc
    47161 aagcgcggca ggcgcgttat caggcatttg cccgtaccct attgcccggc gaagtgctgg
    47221 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
    47281 gcggccctgc cgggctttcg gctatggggg aagtctcgga gtttgccgga acacggctta
    47341 tccgcccgtt gcttgcccgc acgcgggggg aactggcgca gtgggcattg gcgcatggtt
    47401 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
    47461 tgcgcgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
    47521 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
    47581 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctagcg atgagtgatg
    47641 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
    47701 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
    47761 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaact
    47821 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
    47881 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
    47941 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
    48001 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
    48061 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
    48121 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
    48181 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggac
    48241 tcgctcacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgt
    48301 agctcacggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
    48361 aaatcgctgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
    48421 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgta
    48481 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
    48541 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
    48601 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
    48661 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
    48721 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
    48781 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
    48841 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
    48901 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
    48961 cgtatgatgc tgcgcttgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
    49021 cgtggtttgt gtgccgatga tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
    49081 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
    49141 gccgcagaag tggtgctgtt tacctataat agtcgggcgg cgcaaatctg gtggcagcaa
    49201 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
    49261 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
    49321 ggcgtgatct ggttatcgga cgataagaat aatctggaag ttaacttaac cgcctggcaa
    49381 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
    49441 ccgccattcg tgcgcagggc gtgggcgggc agcatgttaa taagacctca acggctattc
    49501 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctactcg
    49561 ccgccagcca tcatttgatc agcagtgatg gcgtgattgt cattaaggca caggaatacc
    49621 gcagtcagga actgaaccgc gaagccgcgc tagcccggct ggtggcagtg attaaagatt
    49681 taacaacaga acaaaaagcc cgacgaccca cgcggcccac ccgtgcatcg aaagagcgca
    49741 ggctggcatc gaaagcacaa aaatcaagcg tgaaggcgat gcgcggcaaa gtacgcagcg
    49801 gtcgggaata aaaagaagga atagatggtg aaaaaagcga tagtgacagc gatggctgta
    49861 atcagcctct ttactctgat gggatgtaat aatcgggccg aagttgatgc gctttctccg
    49921 gcgcaggctg ccgaactgaa accgatgccg caaagttggc gcggcgtgct gccgtgtgct
    49981 gattgcgaag gaatcgaaac ctctctgttc ctcgaaaaag acggaacatg ggtgatgaat
    50041 gagcgttatc tcggtgctcg tgaagaacct tcctccttcg cttcctacgg tacatgggcg
    50101 cgaaccgctg acaagctggt attaaccgac agcaaaggtg aaaagtcata ttatcgggcg
    50161 aaaggcgatg cgctggagat gctcgatcgt gaaggcaatc cgattgaatc gcagttcaac
    50221 tatacgctgg aaccggcaca atccagttta cctatgacgc cgatgaccct gcggggcatg
    50281 tatttttata tggctgatgc ggcgaccttc actgattgcg cgaccggaaa acgtttcatg
    50341 gtagcgaata acgcagagct ggagcgtggc tacctggctg cgcgcggcca cagtgaaaaa
    50401 ccgatgttac tgtcagtaga aggtcacttt acgcttgaag ctaatccgga taccggtgcg
    50461 ccgactaaag tattggcacc cgatacggca gggaaatttt acccaaacca ggattgcagt
    50521 agtttggggc agtaaacctg cttaaggcag aaacaaacgc aaaattgcct gatgtgctgc
    50581 gcttatcagg cctacagcat ataatgcaag attttgaatt agcacgattt tgtaggccgg
    50641 ataaggcgtt tacgccgcat ccggcatttt gtgcacgagg ctggctttat cactgagtca
    50701 gcccaacaaa gcgacccgcc ttgatataaa tcccaggctt caggtgccca acatactgta
    50761 actgcgtttc tggcttaaac gcagagacat caccaatacg catatgcatc agatcggcag
    50821 ggctaatcca gcccgattgc gccagtgtaa gtggatgccc ggctttggca aaggcatccg
    50881 tgacaaattc cgaacaaaac cacgactttt tgtctccttc gcccacactg cttaactgcg
    50941 ctttcgccag gccgctgacg cactttggcg aaaatcctcg gagaacggat tcagtgagca
    51001 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataaccgct
    51061 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
    51121 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacaatctg
    51181 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agaaagattg caacgtgact
    51241 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
    51301 cagcaaatcg ccgggtttga gatcaggtgc cgttatttct tttattgatt gttcggtgaa
    51361 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
    51421 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
    51481 aaacaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaaagggc
    51541 cctgaaattc aggacccttt ctggcatcag cctttaatct gtttcaccag atattcgacg
    51601 atgtcaccag tcttaattaa ctgtttctcg ccgttgcgac gatatttata ttcgatatcg
    51661 tcgttgtcga ggttacggtc gcccagcaca atagtgtgcg gaataccgat cagttccata
    51721 tcagcaaaca tcacgcccgg acgctctttg cggtcatcca gcagcacttc gataccttgt
    51781 gcacgcagtt cgctgtacag tttctcagca agctcttgta cgcggaagga tttgtgcatg
    51841 ttcatcggca gaatcgccac ctggaacggc gcgatagcgt caggccatac gatgcctcgt
    51901 tcgtcgtagt tctgctcaat cgccgcagct accacacgcg ttaccccgat accgtagcaa
    51961 cccatcgtca ggatttggtt acggccatct tcaccctgta cggaggcttt cagtgcttcg
    52021 gagtacttgg tacccagctg gaagatgtga ccaacttcga taccacgttt gatcagcagc
    52081 gtaccctggc catccgggct tggatcgcca gccaccacgt tacggatatc tgcaacttcc
    52141 ggggtagcga catcgcgatc ccagttgatg ccgaagtagt gtttaccatc gatgttagca
    52201 ccagcagcga aatcactcat cgccgcaacg gtacggtcaa tcaccaccgg aatcggcatg
    52261 tttaccggac ccagtgaacc cggaccggct ttaaccacgg cacgaatttc ttcttcggtc
    52321 gcgaaagtca gcgggcttgc aacctgcggc agtttttctg ctttaacttc gttcagctcg
    52381 tgatcaccgc gcaccagcag cgcaaccagc gggaagctac tgccttcaac cgctttaacc
    52441 agcagagtct taaccgtttt ctcaatcggc agattgaact gttcaaccag ttccgcgatg
    52501 gttttcgcgt tcggcgtatc aaccagcgtc atttcctggg tagcagcagc gcgcggttct
    52561 ttcggcgcga tagcttctgc cagttcaatg ttcgctgcat agtcagaggt gtcggagaag
    52621 accacatcgt cttcaccgct ctgcgccagc acctggaatt cgtgagaggc gctgccgccg
    52681 atagaaccgg tgtcggcttg tacggcgcgg aaatccagcc ccatgcggct gaagattttg
    52741 ctgtaggccg catacattgc atcgtaggtt tcctgcaggg attcctgaga agtatggaaa
    52801 gagtaagcat ctttcatcag gaattcgcgg gaacgcatga cgccgaaacg cggacgcact
    52861 tcgtcgcgga acttggtctg gatctgatag aagttcagcg gcagctgttt gtaagagcta
    52921 agctcgttac gaatcaggtc agtgataact tcttcatgag ttgggccgag tacgaacgga
    52981 cgctcgccac ggtcaacaaa acgcagcagt tccggaccgt actgttccca acgaccactc
    53041 tcttgccaca aatcagctgg ctgaaccacc ggcatcgaca cctcgatcgc accggcgttg
    53101 ttcatctctt cacgcacgat gttttcgact tttttcagaa cgcgcacgcc ggtcggcagc
    53161 caggtatata acccggaggc cagcttgcgg atcatcccgg cgcgcagcat cagctgatgg
    53221 ctgatcacct cggcgtcggc aggtgtctcc ttgagagtgg agagcaggta ttggctagta
    53281 cgcatgttgt tacggttcca gttggaaggt agaacaggct caaggcgagc ctgggacaaa
    53341 aaaaagtgat ttagtttacc agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa
    53401 cgcggttcca gcgcaaagac ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa
    53461 tcatgtagcc agacggcata ggttttgccc gtttcctcac ctttacgata ggccgggcgt
    53521 gggtcctgcg ccagtacttc gcggataaac agcgttaact gcggataacg cttctccagc
    53581 gtcaaaagct gcttttcgac ctctgcggta aaactcaccg ccatctctgc cgctggcgca
    53641 ctttgcgcat agctggcact ggcatcggga agcgattcgg caaaggggag atacggtttg
    53701 atatccacta ccggcgtacc atcgaccaga tccagactgc cgagcttcag aatcacgctg
    53761 tctttatggc aaacaacctc tttcagctct accagcgaca taccaattgg gttagggcgg
    53821 aaagtagagc gtgttgcgaa aacccccatt ctggcgttac cgccgaggcg cggtggacgc
    53881 acagtcggac gccagccgcc ttccatcgtt tgatgaaaga cgaaaaggat ccataaatgg
    53941 ctgaacgctt ccaggccgcg aacggcgtcg gcctggttgt agggagcaat gagatgcagt
    54001 tcgccgttgg cgctttttac cagacctggc tggcgcggaa cggcgaattt ttctttatag
    54061 ggcgagcgaa taacgcctat ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa
    54121 gcgcagaacc gatacataca gcctgacgat agcagcctgg cgtaccgctg gtgacttcgc
    54181 agctatgcag taataccgca ttggccttca ttttcgaggc gttgatttgc atccgcttac
    54241 gtgcggttgg aatgctcggc ggagagtcct gattggaggc ctggcaagaa tcgccactga
    54301 cttcaccgag atcgcggaac ggtttgccga ctaattcttc tgcattggta taaattcgga
    54361 ccggcgtggc gcgcggcgct ttcggttttg caggctccgc tttcggctgg ggtgcagtgc
    54421 tttgaacggg ttcgacaggg gatctgctta acatggaaca gccgcttagc atgagtgcta
    54481 ctaaacagat cggtaaagca cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat
    54541 tgaatcagga gcttgtaaaa atgacaagac gggcaagcgc ccgtcctgaa tgatattaca
    54601 aattgtggaa acagcctaaa aattaccagc ctttaacagc gccgccgtta aacactttgt
    54661 ttgctgcttc gtaaacttca tcagactgat aagcctggac gaatttcttc acgttctcgg
    54721 cgtctttgtt atcttcacgc gttacgatca ggtttacgta cggggagtct ttatcttcga
    54781 caaagatacc gtctttcgct ggagtcaggc caatctggct ggcataggtg gtattgataa
    54841 ctgccagagc gatttgcgcg tcgtccagag atcgaggcag ctgcggtgct tccagctcaa
    54901 caattttcag attttttggg ttctcaacaa catcaagaac ggtcggcagc aggccaacgc
    54961 catctttcag tttgatcaag cccacttttt gcagcagcag cagtgaacga ccaagattag
    55021 ttggatcgtt tggcacggca acctgcgaac catcctgcag ttcatccagt gatttgattt
    55081 tcttggagta accagcaatc ggataaacaa aagtgttgcc tactgcgacc agtttgtagc
    55141 cacgatcttt cagttgctga tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat
    55201 cgcctttgct caatgcttcg tttggcagaa catagtcgtt gaaggttacc agctcaacgt
    55261 ccaggccata tttgtctttc gcaactttct gcgcgacttc tgctacctgc tgttcggcac
    55321 caacaatcac gccgacttta atgtggtttg gatctttttc atcctgaccg cagcctacca
    55381 gtgccagtga tccaatcagg gctcccactg ccgcaaaggt tttgaatttg aacgccatac
    55441 cttattcctt cttcaattat ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga
    55501 cgatgcggtc gcctgcgaac tgaattaaat aaaccagaat gaccagcaat accagtaccg
    55561 tattcatcac cgtcgcgtta tagccgatgt agccatactg atagccaatc tgacctaaac
    55621 caccggcacc gactgcacca cccatcgcgg aatagccgac cagggtaatc agggtgatag
    55681 ttgccgcatt caccagaccc ggcagtgctt ccggtaacag caccttacgg acgatctgca
    55741 tcggcgtggc acccattgcg cgggaagctt caattaaccc ggttgggatc tccagcagag
    55801 cgttctcgac catacgggca ataaacggtg ctgcaccaac ggttaacgga acaatcgctg
    55861 cctgcaaacc aatcgatgta ccgacaataa cgcgggtaaa cggaatcatc cataccagca
    55921 agataatgaa cgggatggaa cggaaaatgt tcacaatcgc agaaacggta cgatacagct
    55981 tcgcgttagc aataatttgc cccgggcgcg taacataaag caggacgccg accggcagac
    56041 caatcacaaa gccaaaaaat ccggatacga aggtcattgc cagcgtttcc catacgccac
    56101 gaaccagcaa ccacatcatc ggctcagaca taacccagta cctctacttt tacatggtgt
    56161 tcctgcagcc aggcaatggc ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc
    56221 atgatgccga acttaacgcc accggcgtaa tccatctgcg cgctaataat gttgttgttg
    56281 acgttgaaac gacgcgcggt ttcagaaagc agcggggcat cgaccgattg accggtaaac
    56341 tccaggcgca acatcggcac gcagtcagta aatggctccg cttgcagacg ttcctggtaa
    56401 tcttccggga tatccagatg cagggtcgac tgaataaact tctgcgccag cggcgttttc
    56461 ggatgcgaga acacttcact taccgtgtcc tgctcgatca gttcgccatt gctgatgacc
    56521 gccacgcaat cacaaatacg cttcacaacg tccatttcgt gggtgatcaa cagaatcgtc
    56581 aaacccagac ggcggttaat gtctttcagc agttcgagaa tagaacgtgt cgttgccggg
    56641 tccagcgcgc tggtggcttc atcacacagc aatactttgg gattgctggc taatgcacgg
    56701 gcaatcgcca cacgttgttt ctgcccaccg gaaagattcg acgggtagct atcatgctta
    56761 tcgccaagac caaccaatga cagcaattcc gtcacgcgac gtttgatctc gtctttcggt
    56821 gtgttgtcca gctccagcgg cagagccacg ttgccaaaaa cagtacgcga agagagcagg
    56881 ttaaaatgct ggaaaatcat accaatctgg cggcgagctt tggtcaactc ggattctgac
    56941 agcgtggtca gttcctggcc atcgaccagc acgctaccct cggttgggcg ctccagcagg
    57001 tttacacaac gtataagcgt actcttaccc gcgcctgagg caccgataac gccataaatt
    57061 tgtccagctg gcacatgcag gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg
    57121 tggaacactt tggtgatatt cgaaagtttt atcattgatt atttattatc gtcattaagt
    57181 tagtcgtggc atctcgaatg cctgaaacgg gcaacgccgt caatgaaatg gatgttaagg
    57241 catccagacg tctaaatcaa tcaggtttat gcgaagagca ctttcttgca ggtcgaaaca
    57301 tgcgatacta gcgtcccatg ccttattaag gagctataaa aggtggcgaa gagcgtaccc
    57361 gcaatttttc ttgaccgtga tggcaccatt aatgtcgatc acggctatgt ccatgagatc
    57421 gacaactttg aatttatcga cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc
    57481 tttgcgctgg tggtagtaac caaccagtct ggcattgctc gcggtaaatt taccgaagca
    57541 cagtttgaaa cgctgaccga gtggatggac tggtcgctgg cggaccgaga tgtcgatctg
    57601 gatggtatct attattgccc gcatcatccg cagggtagtg ttgaagagtt tcgccaggtc
    57661 tgcgattgcc gcaaaccaca tccggggatg cttttgtcag cacgcgatta tttgcatatt
    57721 gatatggccg cttcttatat ggtgggcgat aaattagaag atatgcaggc agcggttgcg
    57781 gcgaacgtgg gaacaaaagt gctggtgcgt acgggtaaac ctattacacc tgaagcagaa
    57841 aacgcggcag attgggtgtt aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag
    57901 caaaaaccgg cgcaatgatt aaaagatgag cggttgaaat aaaaatgcat ttttccgctt
    57961 gtcttcctga gccgactccc tataatgcgc ctccatcgac acggcggatg tgaatcactt
    58021 cacacaaaca gccggttcgg ttgaagagaa aaaatcctga aattcagggt tgactctgaa
    58081 agaggaatgt gtaatatacg ccacctcgcg acagtgagct gaaagc
//
LOCUS       DAZGVQ010000008        57080 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000008 DAZGVQ010000000
VERSION     DAZGVQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.008"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(165..896)
                     /gene="rlmB"
                     /locus_tag="ACHLAG_000461"
     CDS             complement(165..896)
                     /gene="rlmB"
                     /locus_tag="ACHLAG_000461"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFP0653768.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLVLVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1076..3517)
                     /gene="rnr"
                     /locus_tag="ACHLAG_000462"
     CDS             complement(1076..3517)
                     /gene="rnr"
                     /locus_tag="ACHLAG_000462"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFP0653769.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3556..3981)
                     /gene="nsrR"
                     /locus_tag="ACHLAG_000463"
     CDS             complement(3556..3981)
                     /gene="nsrR"
                     /locus_tag="ACHLAG_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFP0653770.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKSASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4186..5484)
                     /gene="purA"
                     /locus_tag="ACHLAG_000464"
     CDS             complement(4186..5484)
                     /gene="purA"
                     /locus_tag="ACHLAG_000464"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFP0653771.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5588..5785)
                     /gene="yjeT"
                     /locus_tag="ACHLAG_000465"
     CDS             complement(5588..5785)
                     /gene="yjeT"
                     /locus_tag="ACHLAG_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFP0653772.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5867..6871)
                     /gene="hflC"
                     /locus_tag="ACHLAG_000466"
     CDS             complement(5867..6871)
                     /gene="hflC"
                     /locus_tag="ACHLAG_000466"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFP0653773.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6874..8133)
                     /gene="hflK"
                     /locus_tag="ACHLAG_000467"
     CDS             complement(6874..8133)
                     /gene="hflK"
                     /locus_tag="ACHLAG_000467"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFP0653774.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8219..9499)
                     /gene="hflX"
                     /locus_tag="ACHLAG_000468"
     CDS             complement(8219..9499)
                     /gene="hflX"
                     /locus_tag="ACHLAG_000468"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFP0653775.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9575..9883)
                     /gene="hfq"
                     /locus_tag="ACHLAG_000469"
     CDS             complement(9575..9883)
                     /gene="hfq"
                     /locus_tag="ACHLAG_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFP0653776.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9969..10919)
                     /gene="miaA"
                     /locus_tag="ACHLAG_000470"
     CDS             complement(9969..10919)
                     /gene="miaA"
                     /locus_tag="ACHLAG_000470"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP0653777.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10912..12759)
                     /gene="mutL"
                     /locus_tag="ACHLAG_000471"
     CDS             complement(10912..12759)
                     /gene="mutL"
                     /locus_tag="ACHLAG_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFP0653778.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12769..14106)
                     /gene="amiB"
                     /locus_tag="ACHLAG_000472"
     CDS             complement(12769..14106)
                     /gene="amiB"
                     /locus_tag="ACHLAG_000472"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFP0653779.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14125..14586)
                     /gene="tsaE"
                     /locus_tag="ACHLAG_000473"
     CDS             complement(14125..14586)
                     /gene="tsaE"
                     /locus_tag="ACHLAG_000473"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFP0653780.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14558..16105)
                     /gene="nnr"
                     /locus_tag="ACHLAG_000474"
     CDS             complement(14558..16105)
                     /gene="nnr"
                     /locus_tag="ACHLAG_000474"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFP0653781.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16104..17243
                     /gene="queG"
                     /locus_tag="ACHLAG_000475"
     CDS             16104..17243
                     /gene="queG"
                     /locus_tag="ACHLAG_000475"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFP0653782.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLIGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17226..17279)
                     /locus_tag="ACHLAG_000476"
     CDS             complement(17226..17279)
                     /locus_tag="ACHLAG_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0653783.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17513..17588)
                     /locus_tag="ACHLAG_000477"
     tRNA            complement(17513..17588)
                     /locus_tag="ACHLAG_000477"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17553..17555),aa:Gly,seq:gcc)
     gene            complement(17625..17700)
                     /locus_tag="ACHLAG_000478"
     tRNA            complement(17625..17700)
                     /locus_tag="ACHLAG_000478"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17665..17667),aa:Gly,seq:gcc)
     gene            complement(17911..18456)
                     /gene="orn"
                     /locus_tag="ACHLAG_000479"
     CDS             complement(17911..18456)
                     /gene="orn"
                     /locus_tag="ACHLAG_000479"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFP0653784.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18551..19603
                     /gene="rsgA"
                     /locus_tag="ACHLAG_000480"
     CDS             18551..19603
                     /gene="rsgA"
                     /locus_tag="ACHLAG_000480"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFP0653785.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19700..20668
                     /gene="asd"
                     /locus_tag="ACHLAG_000481"
     CDS             19700..20668
                     /gene="asd"
                     /locus_tag="ACHLAG_000481"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFP0653786.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMANLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20690..24013
                     /gene="mscM"
                     /locus_tag="ACHLAG_000482"
     CDS             20690..24013
                     /gene="mscM"
                     /locus_tag="ACHLAG_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFP0653787.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VIEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24044..24358)
                     /gene="yjeO"
                     /locus_tag="ACHLAG_000483"
     CDS             complement(24044..24358)
                     /gene="yjeO"
                     /locus_tag="ACHLAG_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFP0653788.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDIRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(24355..24669)
                     /gene="yjeN"
                     /locus_tag="ACHLAG_000484"
     CDS             complement(24355..24669)
                     /gene="yjeN"
                     /locus_tag="ACHLAG_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFP0653789.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLLDIYCAYLIRCLVFRGELVGYGDLFHMRYSEIKRPV
                     TL"
     gene            complement(24721..26223)
                     /gene="yjeM"
                     /locus_tag="ACHLAG_000485"
     CDS             complement(24721..26223)
                     /gene="yjeM"
                     /locus_tag="ACHLAG_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFP0653790.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNAQHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHLSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(26442..27419)
                     /gene="epmA"
                     /locus_tag="ACHLAG_000486"
     CDS             complement(26442..27419)
                     /gene="epmA"
                     /locus_tag="ACHLAG_000486"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFP0653791.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27744..29552
                     /gene="frdA"
                     /locus_tag="ACHLAG_000487"
     CDS             27744..29552
                     /gene="frdA"
                     /locus_tag="ACHLAG_000487"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFP0653792.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            29545..30279
                     /gene="frdB"
                     /locus_tag="ACHLAG_000488"
     CDS             29545..30279
                     /gene="frdB"
                     /locus_tag="ACHLAG_000488"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP0653793.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            30290..30685
                     /gene="frdC"
                     /locus_tag="ACHLAG_000489"
     CDS             30290..30685
                     /gene="frdC"
                     /locus_tag="ACHLAG_000489"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP0653794.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30696..31055
                     /gene="frdD"
                     /locus_tag="ACHLAG_000490"
     CDS             30696..31055
                     /gene="frdD"
                     /locus_tag="ACHLAG_000490"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP0653795.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            31118..32251
                     /gene="ampC"
                     /locus_tag="ACHLAG_000491"
     CDS             31118..32251
                     /gene="ampC"
                     /locus_tag="ACHLAG_000491"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP0653796.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAKTYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            32340..32873
                     /gene="blc"
                     /locus_tag="ACHLAG_000492"
     CDS             32340..32873
                     /gene="blc"
                     /locus_tag="ACHLAG_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP0653797.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(32870..33187)
                     /gene="sugE"
                     /locus_tag="ACHLAG_000493"
     CDS             complement(32870..33187)
                     /gene="sugE"
                     /locus_tag="ACHLAG_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFP0653798.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(33362..33508)
                     /gene="ecnB"
                     /locus_tag="ACHLAG_000494"
     CDS             complement(33362..33508)
                     /gene="ecnB"
                     /locus_tag="ACHLAG_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFP0653799.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(33619..33744)
                     /gene="ecnA"
                     /locus_tag="ACHLAG_000495"
     CDS             complement(33619..33744)
                     /gene="ecnA"
                     /locus_tag="ACHLAG_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFP0653800.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(33796..34362)
                     /gene="efp"
                     /locus_tag="ACHLAG_000496"
     CDS             complement(33796..34362)
                     /gene="efp"
                     /locus_tag="ACHLAG_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP0653801.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            34404..35432
                     /gene="epmB"
                     /locus_tag="ACHLAG_000497"
     CDS             34404..35432
                     /gene="epmB"
                     /locus_tag="ACHLAG_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFP0653802.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAKTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            35822..36690
                     /gene="yjeJ"
                     /locus_tag="ACHLAG_000498"
                     /pseudo
     CDS             35822..36690
                     /gene="yjeJ"
                     /locus_tag="ACHLAG_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
     gene            complement(36893..37246)
                     /gene="yjeI"
                     /locus_tag="ACHLAG_000499"
     CDS             complement(36893..37246)
                     /gene="yjeI"
                     /locus_tag="ACHLAG_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFP0653803.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(37384..39030)
                     /gene="groL"
                     /locus_tag="ACHLAG_000500"
     CDS             complement(37384..39030)
                     /gene="groL"
                     /locus_tag="ACHLAG_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP0653804.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(39074..39367)
                     /gene="groS"
                     /locus_tag="ACHLAG_000501"
     CDS             complement(39074..39367)
                     /gene="groS"
                     /locus_tag="ACHLAG_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP0653805.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            39643..40899
                     /gene="yjeH"
                     /locus_tag="ACHLAG_000502"
     CDS             39643..40899
                     /gene="yjeH"
                     /locus_tag="ACHLAG_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFP0653806.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(40915..41391)
                     /gene="fxsA"
                     /locus_tag="ACHLAG_000503"
     CDS             complement(40915..41391)
                     /gene="fxsA"
                     /locus_tag="ACHLAG_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFP0653807.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            41728..43164
                     /gene="aspA"
                     /locus_tag="ACHLAG_000504"
     CDS             41728..43164
                     /gene="aspA"
                     /locus_tag="ACHLAG_000504"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFP0653808.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            43282..44583
                     /gene="dcuA"
                     /locus_tag="ACHLAG_000505"
     CDS             43282..44583
                     /gene="dcuA"
                     /locus_tag="ACHLAG_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFP0653809.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            44699..45037
                     /gene="cutA"
                     /locus_tag="ACHLAG_000506"
     CDS             44699..45037
                     /gene="cutA"
                     /locus_tag="ACHLAG_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFP0653810.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            45013..46710
                     /gene="dsbD"
                     /locus_tag="ACHLAG_000507"
     CDS             45013..46710
                     /gene="dsbD"
                     /locus_tag="ACHLAG_000507"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFP0653811.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITSEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVTNNAASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILVLPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            46747..47322
                     /gene="yjdC"
                     /locus_tag="ACHLAG_000508"
     CDS             46747..47322
                     /gene="yjdC"
                     /locus_tag="ACHLAG_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0653812.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMIAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVSRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            47429..47504
                     /locus_tag="ACHLAG_000509"
     tRNA            47429..47504
                     /locus_tag="ACHLAG_000509"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47462..47464,aa:Phe,seq:gaa)
     gene            47702..47861
                     /locus_tag="ACHLAG_000510"
                     /pseudo
     CDS             47702..47861
                     /locus_tag="ACHLAG_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            48121..49659
                     /gene="cadC"
                     /locus_tag="ACHLAG_000511"
     CDS             48121..49659
                     /gene="cadC"
                     /locus_tag="ACHLAG_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFP0653813.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            50025..51359
                     /gene="cadB"
                     /locus_tag="ACHLAG_000512"
     CDS             50025..51359
                     /gene="cadB"
                     /locus_tag="ACHLAG_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFP0653814.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            51439..53586
                     /gene="cadA"
                     /locus_tag="ACHLAG_000513"
     CDS             51439..53586
                     /gene="cadA"
                     /locus_tag="ACHLAG_000513"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFP0653815.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            53645..55102
                     /gene="dtpC"
                     /locus_tag="ACHLAG_000514"
     CDS             53645..55102
                     /gene="dtpC"
                     /locus_tag="ACHLAG_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFP0653816.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            55339..56856
                     /gene="lysS"
                     /locus_tag="ACHLAG_000515"
     CDS             55339..56856
                     /gene="lysS"
                     /locus_tag="ACHLAG_000515"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP0653817.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(56975..>57080)
                     /gene="ghoT"
                     /locus_tag="ACHLAG_000516"
     CDS             complement(56975..>57080)
                     /gene="ghoT"
                     /locus_tag="ACHLAG_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFP0653818.1"
                     /translation="FISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF"
BASE COUNT        13800 a        15020 c        14215 g        14045 t
ORIGIN      
        1 tgcagcgccg tcgccagcgc caacggattc catgtcatat attattccat atagatttag
       61 tttaaatatt aataaaatga atatttgcag tacgtaatta tcttaccagc tatagacaaa
      121 aaaaaaccat ccaaatctgg atggcttttc ataattcaga cgaactagct gcgctgacga
      181 accgcttcaa ataagcaaat tccggttgca accgaaacgt tcagggaaga aacacttcct
      241 gccattggga tgctgatcaa ctcatcgcaa tgttcacggg tcaggcgacg cataccttca
      301 ccttccgcgc ccatcaccag caccaggcgt ccggtcattt tgctctgata gagtgtatga
      361 tccgcctcgc ctgccgtacc gacgatccag atattctctt cctgcaacat acgcatggtg
      421 cgcgcaaggt tagtcacccg aatcagtgga acgctttctg ccgcgccgca ggctactttt
      481 ttcgccgtag cgttgagctg tgcggagcga tctttcggca caatcaccgc gtgaacgcca
      541 gcagcgtccg cgctacgcag gcacgcgccg aggttgtgcg gatcggttac accgtcgagg
      601 atcagcagga acggttgatc gagcgaagcg atcagatccg gcagatcgtt ttcctgatac
      661 tgacgtcctg gcttcacgcg ggcgataatg ccctgatgca cggcaccgtc gcttttctcg
      721 tcgagatatt ggcggtttgc caactggata accacgccct gagactcaag ggcgtgaatc
      781 agcggtaaca gacgtttatc ttcacggcct tttaaaataa agacttcctg aaaacgttca
      841 ggggcgcgct ccagcagggc ctgcactgcg tggatgccgt aaatcatttc gctcattaat
      901 gtactcgttg tttacgtttt gcctgatgca ctacgtttat ccggcctaca tgatctctgc
      961 aatatattga atttgcgtga ttatgtaggc cggataaggc gttcacgccg catccggcat
     1021 gaacaaagcg cattttgtca gcaatctaac cctcttcttt taaagagggt attgatcact
     1081 ctgccacttt tttcttcgcc gcacgcttcg ctttggtcgc tgcggctatt ttctgcgttt
     1141 tcgccgatgg ctttttcgct tttctcgcgt ctttcttcgc cgctttcggc ttcgtttttt
     1201 tctcaccgcg gaaggcgctg tctggctcaa agtttacctt tttaccgacc tgacgacgct
     1261 tgccgccttt tttgcctgca tcgccttttt tcgctttctc gcgcgccgtt ttaccgacgt
     1321 tgcgcggtgc gcgttcactg gagatcagac taaagtcgat tttgcgctcg tccatattaa
     1381 ccgcttcgac tcgaacttcc acgcgatcgc ccaggcgata agtctggccg ctggattccc
     1441 ccatcaggcg ttgccctacc tggtcaaagc gatagtagtc attgtccagc gaagagacat
     1501 ggaccagacc atcaatgaac aagtcgtcca gacggacgaa gaagccaaag ccagtgacgc
     1561 tggaaattac gcctttaaag acgttaccta cctggtcgag catgaagtca cacttcagcc
     1621 agtcagcgac atcgcgcgtt gcttcgtcgg cacgacgttc cgccatcgaa cagtgctgac
     1681 ccagttgcaa catctcttcc atcgaataat ggtagccgcc ggtttcagtg gtgttgccct
     1741 gatgcccctg ctctttcgcc agcagatact taatggcgcg gtgcagcgtc aggtcaggat
     1801 aacgacgaat cggcgaagta aagtgcgcat aggactgcaa tgccaggcca aagtgaccac
     1861 ggttttctgg atcgtaaatc gcctgtttca tcgagcgcag cagcatggtt tgcagcattt
     1921 ctgcatcagg acggtcggca accgactcca gcagttccgc gtagtcacgc ggttccggct
     1981 tgttaccacc cggcagctcc agccccagct ccgccagcac tgaacggaaa gaggtaatcg
     2041 cttcggtgct cggcttgtcg tgaatacgga acagtgccgg ttctttcgct ttctcaacga
     2101 aacgcgccgc cgagatattc gccagaatca tgcactcttc aattaatttg tgcgcgtcgt
     2161 tacgctgggt ctgttcgata cgttcaatac ggcgttcagc gttgaaaatg aacttcgctt
     2221 cttcgctctc aaatgagatc ccaccgcgtt cttcacgggc tttatccagc actttataga
     2281 ggttatgcaa ctcttcgaga tgcttaacca gcggggcgta ctgctcgcgc agatcctgat
     2341 cgccctgcag aatatgccag actttggtgt aggtcagacg cgcgtgagag ctcatcaccg
     2401 cttcgtagaa tttgtagccc gtcaggcggc ctttcgacga aaccgtcatc tcgcacacca
     2461 tacacaggcg gtctacctgc gggttgagcg aacacaggcc gttagagagc acttccggca
     2521 gcatcgggat aacctgcgaa gggaagtaca ccgacgtgcc acggttacgc gcttctctgt
     2581 ccagcggcgt tggcggacgc acatagtagc tgacgtcggc aatcgcgacc cataaacgcc
     2641 agccgccgcc gcgttttttc tcgcagtaaa ctgcatcgtc aaagtcacgg gcgtcttcgc
     2701 catcaatggt gaccagcggt aaatcgcgca gatcaacacg gcccgctttt gcttcttccg
     2761 gcacttcttc tttcagcccg gcaacctgtt gctcaacagc ctgcggccag atgtacggaa
     2821 tttcatgggt acgcagagcg atatcaaccg ccatgccggt gcccatattg tcgcccagca
     2881 cttcgacgat tttacccacc gctttggtgc ggcgagtcgg acgctgagtc agttcgacta
     2941 cgaccacaaa gcccatccgc gcgcccatga tctgatcggg cgggattaag atatcgaagc
     3001 tcagacggct atcgtcagga accacaaagc cgacgcccgc ttcagtaaag tagcgaccaa
     3061 caatctggct ggtttttggc accagtacgc ggacaatacg cgcttcacga cgacctttac
     3121 ggtcagcgcc cagcggctgc gccagcacct gatcgccatg aatgcaggtt ttcatctgct
     3181 cgctggagag atacaaatca tctttacgcc cttcaacccg cagaaagccg tagccatcac
     3241 ggtggccaat aacggtacct ttcaccaggt cgaggcgttc cggcagcgca tagcactgac
     3301 gacgagtgaa gaccagttga ccatcgcgct ccatcgcgcg caggcggcga cgcaggcctt
     3361 caagctgctc ttcgccttca atgtgcagtt ctaccgccag ctcatcacgg ctggccggtt
     3421 tttcacgttt ggttaaatgt tcgaggataa attcccgact agggatggga ttcgcgtatt
     3481 tttcagcttc gcgttcctgg aaaggatctt gtgacatctc ggttcctccg ttgtcatctc
     3541 tgatgaagat tttcgtcact ccaccagcaa taatttataa agcggttgat tctcttcaac
     3601 caaatcggca agcgtgtagt tatccagttc cgtaagaaaa ctttgcacgg ccttagaaag
     3661 cgcctgtttc aaccgacagg caggtgtaat gtggcaaaac tcactgctgc aattcaccag
     3721 cgataagggc tccagctcgc gcaccacatc accaatacgt atcgcactcg ccgatttacc
     3781 caggcgaatg ccgccatttt ttccacgaac agcagtcacg tagccggcac gactaagttg
     3841 attgattatt ttgaccatat gattacggga gacgccgtag acgtcagtca cttcagaaat
     3901 actggtcatc cgcccttctg gcaatgacgc catgtagatc agcgcacgta atccgtaatc
     3961 agtgaaactc gttaactgca catcaacctc aaaagggaaa tcgggaaaaa atacatttat
     4021 attgatgata aaccagccac aagctgtgtc gctaatttat ttcagttcgg ggaaggaaaa
     4081 aagcgaggat ttaacactgt gtcggatagc gtaatggctt aaccgacaca gcaatgtatc
     4141 acactgttag cccggcaggc aaaatatctg ccaggcgtac cagaattacg cgtcgaacgg
     4201 atcgcgcaga atcatggttt cagtacgatc cggaccggta gagatgatat cgatcggcac
     4261 accagtcagc tcttcaatac gcttgatgta gttcagcgcc gcctgcggca gaccgctacg
     4321 atctttcacg ccgaaggtgg attcagacca gcccggcatg gtttcgtaaa tcggctctac
     4381 acctttccag tcgtcagctg ccagcggagt ggtagtcact tcgcgaccat ccggcatacg
     4441 gtaagccacg cagagtttaa cctctttcag gccatccaga acgtccagtt tagtcaggca
     4501 gaagccagac agggagttca gctgtaccgc acgacgaacg gcaacggtgt ccagccagcc
     4561 ggtacgacga cgacgccccg tagttgcgcc gaattcgtta ccctgcttgc agaggaactc
     4621 gccagtttca tcaaacagtt cggtcgggaa cggacctgca cctacacgag tggagtaagc
     4681 tttgaggata cccagaacgt aatcaacata acgcgggccc aggccggaac cggtcgccac
     4741 gccaccagca gtggtgttgg aagaagttac gtacggataa gtaccgtggt cgatatccag
     4801 cagcgtaccc tgcgcacctt caaacatgac gaaatcgcca cgctgacgcg cctggtcgag
     4861 caggtcagaa acgtcaacca ccatagaagt caggatgtcg gcaacagcca tcgtatcatc
     4921 cagaactttc tggtaatcaa ccgcttcagc tttgtagtag ttaaccaact ggaagttgtg
     4981 atattccatc acttctttca gtttttcagc gaaggtttct ttgtcgaaaa ggtcgccaac
     5041 acgcagaccg cgacgtgcta ctttatcttc ataagcaggc ccgataccac gaccggtggt
     5101 gccgatcgct ttcgcgccac gcgctttctc acgcgcgtta tccagcgcaa cgtgataatc
     5161 aaggatcagc ggacatgctt cagacagcag cagacgctca cgaacgggga tgccacggtc
     5221 ttccagttct ttcatctctt tcatcagcgc ggccggagac agcacaacac cgttaccgat
     5281 gatgctggtt acattctcgc ggagaatacc tgatggaata agatggagaa cggttttttc
     5341 accgttgatt acgagagtat ggcctgcgtt gtgaccgccc tggtagcgta caacatattt
     5401 agcccgttca gtcagaagat cgacgatctt acctttacct tcgtcacccc attgggtgcc
     5461 cagtacgacg acgttgttac ccatttttca aaatcaccgt ttgcttaaaa atggattcta
     5521 ccatcgcttt ttcagagtta cagcactttt tgcactcaaa aatgacctcg atcagtctgt
     5581 tttttgttca gccaatcgtt ttcctcaaca tgtagtagac cacaacgccc gcaaccacaa
     5641 gtccaccgcc aaaacgacgt aaaatattat cgggcaaatt ggtcatcgca gagatcatct
     5701 tcttccatgc cttcgggtaa agcatcggcc ctaaaccttc cagtaccaaa accagggcaa
     5761 gcgccagcca gattgtcgaa ttcattcatt atccttatag aaaaagaaaa ccaccgacat
     5821 ccctgaggat gcggtggctt tattgacctg taccgcagtc gttatattaa cgcgttgcgg
     5881 aagtcggcgt cttcatgtag cggaagaaat cgctatccgg gctcatgacc atcacgtcct
     5941 gattgccaga gaagctgttc tcataagcac gcaggctacg gatgaatgcg tagaagtccg
     6001 gatctttact gaatgcatca gcaaacagtt tggctgcttc ggcatcgcct tcaccacgca
     6061 tgatgcggcc ctgacgctca gcttctgcca gcgttctggt cacttcatag tcggcagtcg
     6121 cgcgcagttt ttccgcttct tcctgacctt gtgaacggtg acgacgcgct accgcttcac
     6181 gctcggcgcg catacggttg tagatcgctt cagacacttc ggtcggcagg ttgatctgct
     6241 tgatacgcac atcgacaact tcaataccca gcgccgccat actgttcggg ttgatgaccg
     6301 gaactttgcc cttcgtctca gccgttacgc gctctgccgc ttcggcaatg gcgttatccg
     6361 ctgccggggt agtaacttca tcttctgtac ccgcagaacc ggagttcagc gcgtcacgta
     6421 cttcgagggt cagacgacca cgggaatcgg tgacgatgtc tttcacgtcc aggcgaccaa
     6481 tttcagaacg cagacggtca gagaacttac gtttcaacag cacttccgct tgcgaaatgt
     6541 cgccaccacc cgttgccagg tagtaacggc tgaaatcgct gatgcgccat ttgatgtaag
     6601 aatcgacgat caggtctttc ttctctttgg tcacaaagcg gtcggcctgg ttgtccatgg
     6661 tctgaatacg tgcgtcgagc attttcaccg tttcaatgaa cggtatcttg aaatgcagac
     6721 ccggctcata aaccagaggt ttgttgtcat cgtcacgcag taccttacca aaacgcagcg
     6781 taataccgcg ctcaccttct ttgacgacaa agacagacat gtaaagcact accagcacga
     6841 tgatgataat cgcgataact gacttacgca tcgttattcc ccctgacgct ggtagtcgtt
     6901 acgctgcgcg ttggcgcggc gttggtccat aatatcgccc tgactggtgg acgacgtgtt
     6961 gcttgctcca ctggttgtgg aggaagaggc tggcggcaaa cgcagcagat tgctggcacc
     7021 gttatcgctc ttcgccgcag gggcgttacc acctttcagc atctggtcta acggcagaac
     7081 catcaggttg ccacctttat cgttaaccag cactttgcgg gtgttaccca acactttttc
     7141 catcgtctcg atatacagac gctcgcgagt aatttccggc gcggctttat attccggcag
     7201 aagtttagca aagcgcgcca cttcaccctg agcttccagg atggtctggg ccttgtacgc
     7261 acgcgcctct tcgaggatac gttgcgcctg accgttcgca cgcggctgaa cttcgttggt
     7321 atacgcttct gcttcacgaa tgtattgctg ttcgttttca cgcgcggcaa tcgcatcgtc
     7381 aaacgccgct tttacttctt ccggcggacg agcagcctgg aagttgacgt ccagcagcgt
     7441 gatacccatg tcatacggac ggatagtctc ttccagttcg cgctgagtat cgctacgaat
     7501 cacggtacga ccttccgtca gaatgcggtc catggtgtat ttaccgataa ctccacgcag
     7561 ggcgctgtcg gtagcctgac gcaggctgtc atccgggctg gtcacgctat acagatattt
     7621 ttccggattg gtgacgcggt actgcacgtt catctcaacg cgcactacgt tctcgtccga
     7681 cgtcagcatc acaccagaag cggccagttc acgcacggct tccacgttca ccggtttgac
     7741 ttcgtcgata aacgtcggtt tccagttcag acccggctca accagatggc tgaatttacc
     7801 aaagcgtgtt accacgccgc gttcggcttc tttaatggta tagaaaccac tggccgccca
     7861 gataatgaca atcgctgccg ctgcgatggt aacgacacga ccgccaagct gcgggcgcgg
     7921 gccttgcgat gaactgccac cgccagatcc ggtgccttta ccgccgccca gaccaccgag
     7981 ctttttgctc agtttgcgga agatatcatc taaatcaggt ggcccttgat cgcgaccgcc
     8041 tttgtttcca tttccctcag agttgccgcc aggtttgctg cttccccacg ggtcgcggtc
     8101 ttgtccgtta ttaccgggct gattccacgc catgtttgtg ctccatattt gttatgcggt
     8161 gatccctgtt gtctttcgac gccagaggat atgactccac gcttcagacg ctacgccgtt
     8221 agatcaggta atcgatcaac gccggttctt gtttacagag gcgacgccag tcaacgatcg
     8281 gcatacgaac ttgcagactt acgctgccgt cctcctccat ccactctttt tctattgcct
     8341 gaagctgata aaaacgactt ctcagacgcc cttcctgcgg tggcagacgc aatgtatgct
     8401 gcgccacctc gccggaaagc cgctccgtca aagcctgaaa aagctgtggt atccccgctc
     8461 cggtctgtgc ggaaagccag acacggatcg gtttgttctc ttcgtcccga tcaatacgcg
     8521 gttcgaaatc ttccagcata tcgatcttgt tcatcaccag cagggttggg atctcgtgag
     8581 cgtcgatctc ttcaagaacc gtattcaccg cttcgatgtt ttcttgtaca cgcacatccg
     8641 ccgcatcaat gacgtgcagc agtaatgtgg cttgccgcgt ctcttgtaac gtggctttaa
     8701 atgccgccac cagatcgtgc ggcaggtggc gaataaaccc tacggtatct gcaagtacgg
     8761 tttcaccgac atctgcaacg tcaatacgcc gcaacgtcgg gtcgagggtg gcaaacaact
     8821 ggtctgccgc gtagacccgc gcttcggtga tgcgattgaa aagggtagat ttaccggcgt
     8881 tggtatatcc caccagcgaa acagtaggaa cgtcggcttt gatacgcgat tgccgcccct
     8941 gctcacgctg cttttcaact ctttccaggc gtgactgtat ctgcacgatg cgattacgca
     9001 acaaacgacg gtcggtttcg agctgggttt cacccggacc acgcaaacct atcccgcctt
     9061 tctgtctttc aaggtgggtc cagccacgca ccaggcgcgt agccagatgg cgcagctgcg
     9121 ccagctcaac ctgcaactta ccctcatggg tacgcgcacg ttgggcgaaa atatctaaaa
     9181 taaggccggt gcggtcgata acacgacact cgcacaaacg ctccaggtta cgctcttgcg
     9241 ccgggctcag ggcatggtca aaaagaacga ccgaagcacc cgtcgctttg acagcttccg
     9301 caatttcaac tgctttacct tcacctacaa aatactttgg gtgcggcgct ttacggctac
     9361 cggtaatcac ctgcaatgct tcgacaccgg cggaagagac cagagattca aactcctgga
     9421 ggtcttccat atctttgtct tgcgtaaaat agatgtgtac cagtaccgcc tgctcaccag
     9481 catcataacg gtcaaacaag cgtataaccc tctaaataga tcagcgggga acgcaggatc
     9541 gctggctccc cgtgtaaaaa aacagcccga aaccttattc ggtttcttcg ctgtcctgtt
     9601 gcgcggaagt attctgcgcg ctgctaccat gatggtagtt actgctggta ccgccaccgg
     9661 cgttgttact gtgatgagaa accgggcgag acgggacaac agtagaaatc gcgtgcttgt
     9721 aaaccatctg gctgaccgtg tttttcaaca ggatcacgaa ctgatcaaaa gactcgattt
     9781 gcccttgcag cttaatacca ttcaccaaat aaatagaaac tggaacacgt tcccgacgca
     9841 gtgcgttcag gaacggatct tgtaaagatt gccccttagc cattctctct tttccttata
     9901 tgcttatttg tactttgaac ctttcgattc tgaaaaaatt gcgcacgata cgtctcaatt
     9961 gtacacattc agcctgcgat agcaccaaca acctgtaata cttcgtcacg cgcctgttct
    10021 ggtttttcac tgtcaagcca gtgaacccct tcccaaccac gcagccaggt tatctgccgc
    10081 ttcgccaact gtctcgtggc gcaaacacct cgataaacca tttcatcgta tgagatttcg
    10141 ccttcaaggt aagaccacat ctggcgataa cccacgcaac gaatggaagg caagtccgta
    10201 tgcaaatctc ctcgggcaaa aagcgcccgg acttctgctt caaaacctga agccaacatc
    10261 tgatgaaaac gctgctcaat gcgttgatgg agcagttcac ggctcgccgg ggcgatggcg
    10321 aactgatgca cctgatacgg tagagcgtct cctgacgttt gcgtcagttc cgttaaagtt
    10381 ttacccgaaa tgaaaaaaac ttccagtgcc cgggaaagcc tttgtggatc atttggatga
    10441 atccttgctg ccgcaaccgg atctacctcc tgaagttgac gatgcaatga ctcccaacct
    10501 tgctctgccg cctgttgctc aattctggcc cgcacttccg ggtctgccga cggtagcggc
    10561 gacaaccctt ccagcaatgc cttgaaatac aacatcgtac cgcccactaa cagtgggatc
    10621 cgccccgccg cggtgatatc ggccatttcc gccagcgcat cgcggcgaaa atcagcagcc
    10681 gagtaagcct gcgacggatc gcgaatatcc agcaatcggt gcggcgcggc gagtaactct
    10741 tcagcgttcg gcttcgccgt cccgatatcc atccctttgt aaataagggc agaatcaacg
    10801 cttatcaact ctactggtaa aattttacgc agctcaatgg ctaacgccgt tttaccggag
    10861 gccgtcggcc ccatcaaaaa aatcgcctta ggcaggctcg ccttactgat atcactcatc
    10921 tttcagggct tttatcgccg gatgtaaatc aacagattgt aacagaccac ccggcggcgt
    10981 tttcacaagt tgcggacata accgttccac gtccgccagc agggttatgg cctgtgccat
    11041 tgaccactgc gcatgttcgc tcatcagatt tcgtgcaatc cactgcgcaa tattgccagg
    11101 ttcgaatacg gactgcttcg ccaggtagcc tatcagttca ggaatcaaga tttgtaaatt
    11161 ttgttggcgt aagggtaaag gcacggccct gatggtcaca tgctgtgcat ctgactggaa
    11221 atcaataccc aattccgcca gggcagactg cgctttttct aatgccgatt tttcttcggc
    11281 agaaactttt agccgcaacg gaatcagcag cggctgggcg caaacgggcg cttcacccgg
    11341 cgtcaattgt gcctgacgta gccaacgttc tgccactggc aaggataaaa gtgaaatgtt
    11401 gccatcgcgc tccagcaacg cacagtcgga atggacgata gtcagtaccc gaccaaaact
    11461 ctgactgttc gccgcaagtg caggttcctg cggttccggc gcttttggtt tttgcatcgg
    11521 cgcgggcgtt tgcaaaagct ggcgatacac ttcaccttgc tgtttctggt agcctggctg
    11581 cgcattcggc cagggggcag ccggacgact gcctgatgct ggcgcaggag tgtagcgcgg
    11641 agctaccggc tcacgagctg ccggttctgc aaagtgattg cgccccgccg ccacgcggtt
    11701 ttccggaatg gaacgcggtg caggttgggg ttcatcgtcc agcggtagcg gcgtttccag
    11761 ttgctgttgt agcacgctca gcacgccctg atagataaaa tcatgcacca gacgcgactg
    11821 atggaaacgc acttcgtgtt tggcggggtg cacgttgacg tccacctgat gcggatcgat
    11881 ctccagatac agcacaaatg ccggttgctg atcggccccc agtttgtctt cgcaggcctg
    11941 gcggatcgcg tgattgatca ggcgatcgcg catcatgcga ccgttcacgt agcaatactg
    12001 aatttctgcc agtgcgggcg tggtgtgatt tggatcggcc acccagccgc gtagcgtgag
    12061 atcgccgtgt tgccattcaa tcgccagcgc ttgttcaaga aaagcggtgc cgcaaatcgc
    12121 gcctaagcgc cgttcttttt gcccgccttc cggcactgcg cggtactgac gcacaatttt
    12181 accgttatgc gacaggttga tcgtgacgtc gaaacgcgcc agcgcaatgc ggcggatgat
    12241 ctcatcaatg tggttaaatt cggttttctc ggtgcgcagg aatttgcgcc gcgccggggt
    12301 gttgtagaac agatccagca cctccagcgt cgtccccaca ggatgcgccg ccggttttac
    12361 cgtcacgttc atatcgcgcc cttcggcata ggcctgccag gcttcctgct gttctgcggt
    12421 gcgtgaagtg agcgtcaggc gggaaaccga actgatactc gccagcgcct caccgcgaaa
    12481 gcccaggctg ataatggctt cgagatcgtc cagagaggcg attttactgg tggcatgacg
    12541 cgccagcgcc agcgccagct cgtctttttt gataccgcag ccgttatcac gaatgcggat
    12601 aagtttcgcc ccaccgcgtt cgatatcaat atcgatacgt gtcgcacctg catcgaggct
    12661 gttttccacc agttctttga ctaccgacgc aggtcgctcg accacctcac ctgcggcaat
    12721 ctggttcgcc agttgtggcg gtaagacctg aattggcatc aatcgtcctt agtttggcag
    12781 cgtgcgatct ggcgtcgtca ccgtactggc cgtttgtgcc gttgccccct gcggcgcaga
    12841 ttgcatcgga tgcgcaagga aataattgcg caggcctttg taaatggctt ctgccagctg
    12901 ttgttggtaa tcgtcgctcg ccagcaaacg ttcttcgctg ttgttgctga taaaaccggt
    12961 ttcgaccagt actgatggga tatccggcga acgcagaacg ccgagactcg cgtgttctgg
    13021 tcggcgttta tgtatttcgc caatgcgttg caactgactg atcatactgg tcgctacatc
    13081 ataccctacc cgttgggaat gaccgaactg taaatccagc accgcctggc ttaaataggg
    13141 gtcagactga ctgttcgcca gcacatcacc cgccccaccc agcagctccg actgtttctc
    13201 gtgctgctcc agccagctgg ccatttcgct gttggcgcga cggttagaga gcacccatac
    13261 ggaagcgcca gtcgcactgc ggttcggtgc ggcatcagcg tgaatcgaca cgaggaaatt
    13321 ggcgttttgc ttacgtgcca catcactgcg ccccatcacc gagataaagt aatccccgtc
    13381 acgggttaaa acgcccttaa acatcggatc gtcattgagc aaagtacgca atttacgcgc
    13441 gatggcgatg gtgacatttt tctcccgcgt accaccgggg ccgatagcgc cagggtcctg
    13501 accgccgtgt ccggcatcaa tagcgatgat aattttatcg ccagtgttag ccgtcgcgcg
    13561 cgctgccgga cgcgttaccg tattactgct gataacaccc gtagtgcggt tactttccgt
    13621 tttaaacgga ttgcgcgccg gttcgctgac gcgcggtgcg acaaccgcag gcgtttcaac
    13681 gcgtttcgca accacgggcg gcggaggagg aggtggcggc gcatcggcgt taatcgtaaa
    13741 gacgacagtg taattactgc cattctgccg cttcaccgct tcggttttac cgttttcggt
    13801 aagatcgacc accagccgta gcgtttgtgc atctttaggc gttccagagc gaatcgcctt
    13861 caccagatta ttgccgctga acaacaacgg cagtccctga atcacgcccg tttgtttgat
    13921 atcgagcgcc acggtgcgtt tgctttgatg gctaaacgca taatcaggat cgccaataaa
    13981 actcaacgtt atccgcgcct gttgattacc gttagaaacc tgaatatcag agagcgtcgc
    14041 ggcacccacc ggcgtgcaca gcagcagcag cgtcgctacc aaccaatttc tgatgcgata
    14101 catcatcccg ccacctttca aaggttaacc ggctaaacgc gccagcaaca attcacccgc
    14161 agaggaaacc gcactcacgc gcgcctcacg gccttgtgcc tgataatcaa tgtgtatttc
    14221 gacatccggg tcaggaagaa caccagtacc ttgttgtggc cactccacca ggcagatggc
    14281 atcgttggca aaataatcgc gaatccccat aaactccagc tcctcgggat cggcaaggcg
    14341 gtacaaatca aagtgataaa ccattaaatt gtcgagcgta tagggttcga ccagcgtata
    14401 agtggggctt ttgacattac cctgatgacc cagagcttgt aaaaagcccc ggctaaaggt
    14461 ggttttacct gcgcctaaat cgccatacag atagattacg gttgcgccat cgcaggcttt
    14521 cgctacccgc tcgcccaggt ctaatgttgc ctgctcatca gggagcggaa ttactcgatt
    14581 catcatggtt tttatcagtc acttccgggt taacaatacg ctgtagcgtg gaaaagagat
    14641 cggttgccag catcccgcgc gttccaaaac gcgccgccag tacgtcagct gcagcaccgt
    14701 gcgcgacaca gcccgcacag gcggcatcat acggcgacag tttttgccca agcaatgcgc
    14761 caataatacc agagagcaca tcgcccatgc cgccgctcgc catgcctgca tttccggcat
    14821 caataatgcc taaagcgtca ggatgggcgg cgaccacggt tccggcacct ttcagcaccg
    14881 ctacgccgcc ataacgttgt accagacgtt tggcgcaatg taagcggtca ctttcaattt
    14941 cagcgacgga acagcctaac aaccgtgcgg cctcgccagg atgcggcgtg atcacgcgat
    15001 tgtgacgctt atcgggattg attgccagca ggttcaatgc atcggcatcc cacaacatcg
    15061 gtttgcgaaa attctcaact ttttgcagtg cttttttccc ccactcttgc tggcccagac
    15121 cgggaccaat caccaccaca tcggcccatt ccaggctttc ggtaagagag tccatcgtca
    15181 gttcatgcac catcaattcc ggtcgtgcag tcagcagcgg cgcaatgttt tcactgcggg
    15241 tcagtactcg gactaaacca gcaccagcac gtagcgccgc ttcccccgtc atacgaatag
    15301 cccccgccgt gccgtgatcg ccaccgataa ttaccagccg cccgtgatcg cctttatgcg
    15361 aagtcgggcg acgcggtttt agccagtgag aaagttgttc tgccgaaaac cgctgaattt
    15421 tcgtctcctg acctgccagc caactatcca gccccagtga gtcaaaatgc agttgtccgg
    15481 taacatcccg cgcttttcca gtgagcaagc ctggtttcag cgcaataaaa gtgatggtgt
    15541 gatcggcgtt gatcactgcg cctggcgtag cgccggtttc agccagcagg ccggaaggga
    15601 tatcaaccgc cgcaatcggc gcaggatggg aattagcgtg gtcgattaac tggctaatgg
    15661 attcgcgggg cgcttgctgc aagccggtac cgagcagcgc atcaacaatc agatctaccg
    15721 attcgggcca gacaatattc gaagcatgga tctcgcctcc cgcgtttaac catgcttcgc
    15781 gtgccagcgc ggcctcttcc ggcaacggtt tgtcgctctc ctgggccagc agcgtgacct
    15841 caatgccgac cgctgtggcc agccgcgcga ccacgtagcc atcgccgccg ttattaccat
    15901 gaccgcacag caccagccaa tggcgggcgt caggatacgc cgaacgacac acctggaatg
    15961 cggcttcgcc agcgcgaagc atcagctcat agagtgtaag ccccagcgca tctgccgcct
    16021 cacgttctcc gcggcggata tcgtcggcgt gccagacggt gtgtggtata cttacggggt
    16081 ttttcttcat tgtatggtcc gtcatgtcag agcccctcga tctcaatcag ttagcgcaaa
    16141 aaattaaaca gtgggggctg gaactgggct ttcagcaggt aggtattacc gataccgatc
    16201 tcagcgagtc cgagcccaaa ctgcaagcat ggctggacaa acaataccac ggcgaaatgg
    16261 actggatggc acgtcacggt atgctgcgcg ctcgccccca tgagttattg cccggtacgc
    16321 tgcgcgtgat cagcgtgcgg atgaattacc ttcctgctaa cgccgcattt gccagcacgc
    16381 tgaaaaaccc caaactcggc tatgttagcc gttatgcgct gggccgtgac tatcacaaac
    16441 ttctgcgcaa ccgactcaaa aagctgggcg agatgattca gcaacattgt gtttcgctga
    16501 attttagacc gtttgtcgat tctgcgccta ttctcgagcg cccgttagct gaaaaagctg
    16561 ggctcggctg gacaggtaag cactcactta tcctcaatcg cgaggccggt tcgttcttct
    16621 ttttaggcga attgctggtc gatattccgc tgcccgtgga tcaaccagtc gaggaaggat
    16681 gcggcaaatg cgtggcctgt atgacgattt gcccgaccgg tgccatcgtc gagccatata
    16741 ccgtcgatgc tcgccgctgt atctcttatc tcaccatcga actggaaggg gcgatcccgg
    16801 aagagttgcg accgttaata ggaaaccgta tttacggttg cgatgactgc cagcttatct
    16861 gcccgtggaa tcgctattca caactcacca cagaagagga tttcagcccg cgtaagccgc
    16921 tacacgcacc ggaactcatt gagttattcg cctggagcga agagaagttt ttaaaagtca
    16981 cggaaggatc ggcgattcgt cgtattggtc acctgcgttg gctgcgtaat atcgccgtag
    17041 cattaggcaa tgccccttgg gatgaaacga ttttgacagc gctggaaagt cgtaaaggtg
    17101 agcacccact tctcgatgag cacatagcgt gggcgattgc gcagcaaata gagagacgaa
    17161 atgcgtgcat agtcgaagtg caattgccga aaaaacagcg tctggttcgg gtgattgaaa
    17221 aagggttacc gcgtgacgcc tgattcatcc acagcttgtg aataaaaata aaaacgcatt
    17281 gcaattcaag acgaataaaa tcgtcaagtg atcgactcga caaaacggaa tcttaatttt
    17341 taacttatct atcaataggt taaaaaaata ctattcaccg tgcgaagttt ctttgaaatt
    17401 aagattacag cctgaggctg tggataactc tgtttacaag agtttgtcag agacaacgaa
    17461 aaccagcaat aacgcatcgc atcgctgtgg atattttatt gagagaagaa tttggagcgg
    17521 gaaacgagac tcgaactcgc gaccccgacc ttggcaaggt cgtgctctac caactgagct
    17581 attcccgctt gggtggtctg tgctttgcag cacttttcaa attttggagc gggaaacgag
    17641 actcgaactc gcgaccccga ccttggcaag gtcgtgctct accaactgag ctattcccgc
    17701 gtactactta attttgcttt cgtaattttc gcatttctgc gtcgttacgg gaggcgcatt
    17761 atacgagaaa tctttccctc tgcaaccccc caaaaaagtt ttttttgcgt ttttatctca
    17821 agtgctgaaa ttatcagcaa gacgttcaat ttagcgtcaa aatcacgccg ccgcaagtgg
    17881 attccggcgc ttacgtgacc aggaaaaatt ttacagcttg ataaaatgct cgcggtagta
    17941 agccagctcc gccaccgatt cacggatatc atccatcgcc tgatgcgtcc cctgcttggt
    18001 aaaaccatcc agaatttccg gcttccagcg gcgcgccagc tctttcaggg tgctgacatc
    18061 gagataacgg tagtggaagt aggcttccag ctccggcatg tatttaaaca ggaaacgacg
    18121 gtcctgaccg atgctgttac cgcaaatcgg cgattttccc gcaggcaccc actgttttaa
    18181 aaattcgagc gttgccagtt cagcttcccg atcgcccatc gtgctcgctt tcacgcgctc
    18241 taccagcccg ctggcggtat gggtgcgcac gttccagtca tccatcagcg ccagctgttc
    18301 atcagactgg tgtactgcaa tggtcggccc ttctgccaga atattcaggt tggcatcggt
    18361 caccagcgtg gcaatctcaa taatgcgatc gcgctcggga tccagaccgg tcatctcaag
    18421 atcgatccaa atcaggttgt tttcattggc actcatgcta ttttccaccc attcacagtg
    18481 actatattga tccacaattg cgtgtatcat agatgttttg cccatcaggg gcgaccagga
    18541 gtctgtacga ttgagtaaaa ataaactctc caaaggccag cagcgccgcg tgaacgccaa
    18601 tcaccagcgt cgtcttaaaa cgtctaagga gaagcccgac tacgacgaca atctgtttgg
    18661 cgagcctgat gaaggtatcg tcatcagccg ctttggtatg cacgctgatg tggaatccgc
    18721 cgatggcgac gttcaccgct gcaatattcg ccgtaccatc cgttcgctgg taaccggcga
    18781 ccgcgtagtc tggcgtccgg gtaaaccggc ggcggaaggc gtaaatgtca aagggatcgt
    18841 ggaagcggtg catgagcgca cctcagtgtt gacgcgtccg gatttctacg acggcgtgaa
    18901 acctattgcc gccaacatcg accagattgt cattgtctcc gccattttgc cggagctgtc
    18961 gctcaatatt atcgaccgtt acctggtggc ctgcgaaacc ttgcagattg agccgattat
    19021 tgtgctcaat aagatagacc tgctggacga cgaaggcatg gcgttcgtca acgagcagat
    19081 ggatatctac cgcaacattg gttatcgcgt attgatggtt tccagccata ctcaggatgg
    19141 gctaaaaccg ctggaagagg cgttgaccgg gcgcatcagc atttttgccg ggcagtctgg
    19201 cgtcggcaaa tccagcctgc tgaatgcgtt gctggggctg caaaaagaga tcctgaccaa
    19261 cgatgtctct gacaactcgg ggctcggtca gcacaccacg accgccgctc ggctgtatca
    19321 cttcccgcac ggcggtgatg tgattgattc ccctggtgtg cgtgagttcg gcctctggca
    19381 cctggagccg gaacaaatca ctcagggctt tgtcgaattc catgactatt taggtctgtg
    19441 taaatatcgc gattgcaaac acgataccga tccgggctgc gctatccggg aagcggttga
    19501 ggaagggaaa atcgcggaaa cccgtttcga aaactatcac cgtattctgg aaagcatggc
    19561 gcaggtaaaa acgcgtaaaa acttttctga tacggatgac tgacaattaa gctaaccgtc
    19621 gttagaatcg tccccttttt ttcaggatcc ggcatgttgg ccggatcagg aacgacaaaa
    19681 caatggcctg gaggctacct tgttaaattc atttaaactt tcgctacagt acattctgcc
    19741 gaaactatgg cttactcgcc tggcgggttg gggcgcaagc aagcgggcag gatggctgac
    19801 aaaactggtt atcgatctgt tcgttaaata ctacaaggtc gacatgaaag aggcgcaaaa
    19861 gccggacacc gccagctacc gcacctttaa cgaattcttt gtccgtccgc tgcgtgacga
    19921 agtacgccca atcgataccg atccgaatgt cctggtcatg cctgccgatg gcgttatcag
    19981 ccagttgggt aaaatcgaag aagataaaat cctgcaagcc aaaggccaca actacagcct
    20041 cgaagccttg ctggcaggca actatctgat ggcgaacctg ttccgcaacg gtacgtttgt
    20101 gaccacttac ctctccccgc gtgactacca ccgcgtacac atgccgtgca acggtattct
    20161 gcgtgagatg atctacgtgc cgggcgatct cttctccgtt aaccatctca ccgctcagaa
    20221 cgtgccgaat ctgtttgccc gtaacgaacg cgtgatttgc cttttcgata ccgaatttgg
    20281 ccctatggcg cagattctgg tcggagcgac gattgttggc agcattgaga cggtctgggc
    20341 gggcaccatt acgccgccgc gcgaaggtat catcaagcgc tggacctggc ctgccgggga
    20401 aaacgacggt tctgtggcac tgcttaaagg ccaggaaatg ggtcgcttta aactcggttc
    20461 caccgttatc aacctgtttg caccgggtaa agtgaatctg gttgagcaac tggaaagcct
    20521 gtctgtcacg aaaattggcc agccgctggc agtatctacc gaaacctttg ttacgccaga
    20581 cgctgaacct gccccgcttc ctgctgaaga gatcgaagca gaacacgacg ccagcccatt
    20641 ggttgacgac aaaaaagacc aggtctaatc catcaaagga aacgctgacg tgcgcctgat
    20701 tatcactttt ctgatggcct ggtgcctcag ttggggggcg tacgccgcga cggcccccga
    20761 tagcaaacaa atcacccagg aactggagca ggcaaaagcg gcgaaacccg cgcagccgga
    20821 agtcatagag gcactccagt ctgccttaaa tgcgcttgag gaacgaaaag gttcccttga
    20881 gcgcatcaaa caatatcagc aagttatcga taattacccg aaactctccg ctactctgcg
    20941 cgcacaatta aacaacatgc gtgacgagcc gcgcagcgtg tcgccgggaa tgtctaccga
    21001 tgcgctgaat caggaaattc tccaggtcag cagtcagttg ctggataaga gccgtcaggc
    21061 ccagcaagag caggagcgcg cccgcgagat tgccgattca ctgaatcaac tgccgcaaca
    21121 gcaaaccgat gcccgccgtc agttaaatga gatcgagcgc cgcctgggaa cgcttaccgg
    21181 caatactccg ctcaatcagg cacaaaattt cgcgttgcag tctgactccg cacgcctgaa
    21241 agcgctcgtt gatgaactgg aactggcgca gctgtctgcc aataaccgtc aggaattagc
    21301 gcgcttgcgc tcagagctgg cggaaaaaga gagccagcaa ctggatgcgt atttgcaggc
    21361 cttacgtaat cagttgaata gccaacgtca gcttgaggcg gagcgggcgc tggaaagtac
    21421 cgaattactg gcagaaaaca gtgccgattt gccgaaagat atcgtcgcgc aattcaaaat
    21481 taaccgcgaa ctatcggcgg cactgaatca acaggcgcag cggatggatc tcgttgcctc
    21541 gcaacagcgt caggctgcca gccagacgtt acaggtccgg caggcgttga atacgctgcg
    21601 tgaacagtcg caatggctgg gatcgtccaa cctgctcggt gaagcgctgc gggcgcaggt
    21661 ggcacggcta ccagaaatgc caaaaccaca acagcttgat accgaaatgg cgcagttgcg
    21721 tgtgcaacgg ttacgttatg aggatctgct taataaacag ccgctgctac ggcaaatcca
    21781 tcaggccgac ggtcagccgc tgaccgccga gcaaaaccgt attctggaag cacaactgcg
    21841 cactcagcgt gagttgctga actcattgtt gcagggtggc gacacgctac tactggaact
    21901 gaccaagctg aaagtctcca acgggcaact ggaggatgcg ctgaaagagg tgaacgaagc
    21961 aacgcaccgc tatctgttct ggacctctga cgtgcgcccg atgaccatcg cctggccgct
    22021 ggaaatcgcc caggatctgc gtcgtctcat ttcgctggac accttcagtc agttgggcaa
    22081 agccagtgtg atgatgctga ccagcaaaga gacgattttg ccgctgtttg gcgcgttgat
    22141 tctggtcggt tgcagtattt actcgcgccg ctatttcacc cgttttcttg aacgttcggc
    22201 ggcgaaagtc ggcaaagtga ctcaggatca cttctggctg acgttgcgca ctcttttctg
    22261 gtcgattctc gtcgcgtcac cgctgccggt gctgtggatg acgctgggtt acggcttgcg
    22321 cgaggcgtgg ccttatccgc tggcggtcgc gattggcgat ggcgtaacgg ccaccgtgcc
    22381 gctgctgtgg gtagtgatga tttgcgccac ctttgcccgc ccgaacggct tgtttatcgc
    22441 tcattttggc tggccgcgcg aacgtgtttc ccgtgggatg cgctactacc tgatgagcat
    22501 cgggcttatt gtgccgctga ttatggcgct gatgatgttc gataacctcg acgaccgtga
    22561 attctccggt tcgctgggac ggctttgctt tatcctcatt tgcggtgcgc tggcggtggt
    22621 caccctcagc ctgaaaaagg ccgggatccc gctgtacctc aacaaagagg gcagcggcga
    22681 caacattacc aaccatatgc tgtggaacat gatgattggc gcaccactgg ttgccattct
    22741 ggcgtcagcg gtgggttatc tggcaacggc gcaggcactg ttagcgaggc ttgaaacctc
    22801 ggttgccatc tggttcctgc tactggtggt ttatcacgtt atccgccgct ggatgctgat
    22861 ccagcgtcgc aggctggcgt ttgaccgggc gaagcatcgc cgggcagaga tgttagcgca
    22921 acgcgcgcgt ggcgaagagg aagcgcatca tcacagtagc ccggaagggg caattgaagt
    22981 cgatgaaagc gaagtcgatc tcgatgccat cagtgcgcaa tccttgcggc tggtgcgctc
    23041 aattttgatg ttgatcgccc tgctttctgt cattgtgctg tggtcagaaa tccattccgc
    23101 tttcggcttc ctcgaaaata tttcgctgtg ggatgtcacc tccacggtac agggcgtaga
    23161 aagtctggag ccaattaccc tcggtgcggt gctgattgcc attctggtgt ttatcatcac
    23221 cacgcagctg gtgcgcaacc tgcccgcgct gctggaactg gcgattttgc agcacctgga
    23281 tttaacgccg ggtacgggct acgccatcac caccatcacc aaatatctgc tgatgctgat
    23341 tggcgggctg gtcggcttct cgatgattgg tattgagtgg tcgaaattgc agtggctggt
    23401 cgccgcgctc ggtgttggtc tcggttttgg tttgcaggaa attttcgcca actttatctc
    23461 tggtctgatt atcctgttcg aaaaaccgat tcgcattggc gatacggtga caattcgcga
    23521 tctcaccggt agcgtgacga aaattaacac ccgcgccacc accatcagcg actgggaccg
    23581 taaagagata atcgtgccga acaaggcgtt tattaccgag cagtttatca actggtcgct
    23641 ctctgactcg gttacgcgcg tggtgttgac gattccggcc cctgccgatg ccaacagtga
    23701 agaagtgacg gaaatcctgc tcaccgcagc gcgtcgctgc tcgctggtga tcgacaaccc
    23761 ggcaccggaa gtcttcctgg tggatctgca acaggggatt cagattttcg agctgcgtat
    23821 ttacgccgct gagatgggtc accgtatgcc gctgcgccat gagatccacc agttaattct
    23881 ggcaggcttc cacgcccacg gtatcgacat gccattccca ccgttccaga tgcgtctgga
    23941 aagcctgaat ggtaagcaaa cggggagaac attaacttct gcgggcaaag gtcgtcaggc
    24001 gggaagtttg taaggtcttt gtggccggat gaacccatcc ggctcacaaa caaaactgat
    24061 agcgcagaaa aaactgccac agccagtagc cgaaaatagc cagcgcggtg gcgtacataa
    24121 accagccgcg tttttttcgc cacagcgcga accagagaaa tgggaaaaag agcggtaacg
    24181 tcattatcac accgacatcg cggatatcgt cttcttcgag atacatcaac acatcgcaga
    24241 cgttgttaat cccgcgtcca tcaatcatcc actctttgtc cagcgcggag gtaatggtta
    24301 tccagaggaa cgccacaatg aaccagatgc agcacaagac aaacatgcgc gcactcataa
    24361 ggtaaccggt cgcttaattt cgctatagcg catatgaaac aaatcgccgt atcccactaa
    24421 ttccccgcga aaaaccagac atcggataag ataagcgcaa taaatatcca gcaggtttcc
    24481 ccaccgttta ccatcgcgat ctttgagggt gttaatggtg ctacccacca ccatcgccac
    24541 tttacgggtg tggcaggcat cgacaaaaga gagaatagtc tgctctattt ccgctacatc
    24601 cccggcagaa aattgcagcg ccctgatttc atcctcggtt attgccgggt cccggctggt
    24661 gtcatccata acatttcctg tcattgcgcc ctatcgtcag gcagacgata gggcatcggg
    24721 ttattccacc gcccactgcg gattttttgc cactctgctg caatagttct ggtaaatcgc
    24781 catcgctaac agcgagaaga agacagggcc gccaatcatc cacaatgtgc tgtcccagtc
    24841 tccggcttca accacaggtt gaatgatggt gaagacgttc gcaaatgtca ccaccagtac
    24901 gacaaccact gtcgcaatca ttgccgacaa atgcgttttg aaaatcacaa acggtctgtc
    24961 gagatcctga cgtgctttaa agaacgggaa agccagcgcg aggaacaggt aaggaagcgt
    25021 catagacacg ttcgccatca gcgtcagctt gttaaagaac gccgatgcgg taccgccacc
    25081 aaacgaaacc agcaggatga agacagtaac caacccgcac tgcatccaca tggcgataga
    25141 aggcatcccc atcgcattca ggcgcgtcat cggttccggc cacaacgctt tcggcgtccc
    25201 ctggatgatg gctttcagcg gtgaatagca aagcgtaaag aacgccccgg tataagcgag
    25261 gaacatcgaa agtccggtaa tacgcgcaaa ccatacaccc agcgacaatg acgcttcagg
    25321 tgacaaatgc tgtgcgttac ccagcgtcac cccgaggctc ttcatcagca cataggtaat
    25381 attgccgagg ttaacggaac cattacttaa tacctgctgc cagtttgtgc tgacacccca
    25441 taaaaatatt gccagcgaat aaccgattga aataacaata gcggcaaaaa caataccttt
    25501 ggcaaagttc ttttctggat tttccgtttt atcgaccaga ccaccaaccg cttcaattcc
    25561 gccataggca aaaatagcaa ataccacaaa cgatagcatt gccagaccgg actgataacc
    25621 tgggttcggt gatgcaagga aattaatatc ctgcgcgaaa tgcccaccat ttaataacaa
    25681 aatagtaatg cttactaaca gcaatactaa attcagacac attactgcaa taccgcccac
    25741 cgcagtaatg cgggcaattt tattaatccc ctttgaagca acgacggtga ccagaatcat
    25801 ccatgccact gccagcagac caaccacctg cgtaggctcc agtccagcaa tacgccagtg
    25861 ctgggtcatg tcgctaccat agaggaatgt tgagaacggt acccaaactt tcgcggaggt
    25921 actcaccatc cagatgatat aagaggaaaa ccacataaac gtaccaatga aggcaaaacg
    25981 tggtccgaca ctattattca tccaggaata gataccgcct tcttctttgc gataagcagc
    26041 tcccatttca gccatcatta aggcgaatgg aataaagaat aacaatgcag aaaatatata
    26101 aaagggaatc gcactataac ccattaagta ataagccgat gggctattgg caaatccaaa
    26161 tacggaagta aagatcatca atatgagtcc tatcagactc atctttttta tcgtgtgagg
    26221 catgaaacca tccttcattt cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc
    26281 aatattgagc aggcagcgta aaaaccaata cagaattgat acctggatga tattaaatat
    26341 ccagtcttaa attgtggact ttaacggaaa aatgaaaaca tatgctacag aatggcgctt
    26401 atcacgccat tcttcgctgt taattcagta atttttcaga attatgcccg gtcaacgcta
    26461 aaggcgatga cttcagccag tgtctccgcg cccagcgcca acatcaccag acgatcaaca
    26521 cctaatgcca cgccggaaca gtcaggcata ccgactttca aggcttcaat cagattctgg
    26581 tcaatggggt gctgcggcaa accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt
    26641 tgttgctgct cacgggcatc cgtcaattca tggaaaccat tcgccagctc aataccttta
    26701 taataaacct caaagcgttc agcgacccga tgatcttcgg tactgatttg cgccagtgat
    26761 gcctggctgg ctggaaagtg gtacacaaag gtcggttttt ctttgccaat atttggctct
    26821 acgccaaagg taaacagcaa ttgtagcagc gtgtcgcggt cttcttcggt atcagcaaca
    26881 ttgctcaaat ccagtttcgc tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg
    26941 tcaatttcca gataacgcaa gaaagcttgt tgataagaaa ggctttctgc tgccgggcag
    27001 tccagcacct gttgtaagag atcgtccacc tcgttcatca accggtacat atcatagtgc
    27061 ggtcgatacc actccagcat agtgaactca gggttgtgat aacgccccat ctcttcatta
    27121 cggaagctgc ggcacagctg gaataccggc ccacaaccgg caaccagcag gcgtttcata
    27181 tggtattccg ggctggtcat taaccagaga ttcatcccct gcgaatgccc ggggccaacg
    27241 aaacgtgtct caaacgggac caaatgaata tcggttaccg tcgcctggct catacaaggc
    27301 gtctccacct ccagcactcc acgatcggca aagaaacgac ggatctccgc cataatcgcc
    27361 gcgcgtttta ataagttagg aatggatgcg ctcggctgcc aggatgccgt ttcgctcata
    27421 gttaaatctc cagtttttga caagggcacg aagtctactc gcaacgcgac ggcgagacaa
    27481 attttacgca ggaatcaaac agcggtgggc agtgactaaa aaaagcacga tctgatggtt
    27541 tagtaattaa attaatcatc ttcagtgata atttagccct cttgcgcact aaaaaaatcg
    27601 atctcgtcaa atttcagact tatccatcag actatactgt tgtacctata aaggagcagt
    27661 ggaatagcgt tcgcagaccg taactttcag gcacttaccc tgaagtacgg ggctgtggga
    27721 taaaaacaat ctggaggaat gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg
    27781 ccggtggcgc gggattacgt gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa
    27841 tcgcactaat ctcaaaagta tacccgatgc gtagccatac cgttgctgca gaagggggct
    27901 ccgccgctgt cgcgcaggat catgacagct tcgaatatca ctttcacgat acagtagcgg
    27961 gtggcgactg gttgtgtgag caggatgtcg tggattattt cgtccaccac tgcccaaccg
    28021 aaatgaccca actggaactg tgggggtgcc catggagccg tcgcccggat ggtagcgtca
    28081 acgtacgtcg cttcggcggc atgaaaatcg agcgcacttg gttcgccgcc gataagaccg
    28141 gcttccatat gctgcacacg ctgttccaga cctctctgca attcccgcag atccagcgtt
    28201 ttgacgaaca tttcgtgctg gatattctgg ttgatgatgg tcatgttcgc ggcctggtag
    28261 caatgaacat gatggaaggc acgctggtgc agatccgtgc taacgcggtc gttatggcta
    28321 ctggcggtgc gggtcgcgtt tatcgttaca acaccaacgg cggcatcgtt accggtgacg
    28381 gtatgggtat ggcgctaagc cacggcgttc cgctgcgtga catggaattc gttcagtatc
    28441 acccaaccgg tctgccaggt tccggtatcc tgatgaccga aggctgccgc ggtgaaggcg
    28501 gtattctggt caacaaaaat ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa
    28561 ctccgctggg cgagccgaaa aacaaatata tggaactggg tccacgcgac aaagtttctc
    28621 aggccttctg gcacgaatgg cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg
    28681 tttatctcga cctgcgtcac ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct
    28741 gcgaactggc gaaagcgtac gttggcgtcg atccggttaa agaaccgatt ccggtacgtc
    28801 cgaccgcaca ctacaccatg ggcggtatcg aaaccgatca gaactgtgaa acccgcatta
    28861 aaggtctgtt cgccgtgggt gaatgttcct ctgttggtct gcacggtgca aaccgtctgg
    28921 gttctaactc cctggcggaa ctggtggtct tcggccgtct ggccggtgaa caagcgacag
    28981 agcgtgcagc aactgccggt aatggcaacg aagcggcaat tgaagcgcag gcagctggcg
    29041 ttgaacaacg tctgaaagat ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc
    29101 gcgacgaaat gggcctggca atggaagaag gttgcggtat ctaccgtacg ccggaactga
    29161 tgcagaaaac catcgacaag ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca
    29221 ccgacacctc cagcgtgttc aacaccgacc tgctctacac cattgaactg ggccacggtc
    29281 tgaacgttgc tgaatgtatg gcgcactccg caatggcacg taaagagtcc cgcggcgcac
    29341 accagcgtct ggacgaaggt tgcaccgagc gtgacgacgt caacttcctc aaacacaccc
    29401 tcgccttccg cgatgctgat ggcacgactc gcctggagta cagcgacgtg aagattacta
    29461 cgctgccgcc agctaaacgc gtttacggtg gcgaagcgga tgcagccgat aaggcggaag
    29521 cagccaataa gaaggagaag gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg
    29581 cgctataacc cggaagtcga taccgcaccg catagcgcat tctatgaagt gccttatgac
    29641 gcaactacct cattactgga tgcgctgggc tacatcaaag acaacctggc accggacctg
    29701 agttaccgct ggtcctgccg tatggcgatt tgtggttcct gcggcatgat ggttaacaac
    29761 gtgccaaaac tggcatgtaa aaccttcctg cgtgattaca ccgacggtat gaaggttgaa
    29821 gcgttagcta acttcccgat tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa
    29881 agtctggaag cgatcaaacc gtacatcatc ggcaactccc gcaccgcgga tcagggtact
    29941 aacatccaga ccccggcgca gatggcaaag tatcaccagt tctccggttg catcaactgt
    30001 ggtctgtgct acgccgcgtg cccgcagttt ggcctgaacc cagagttcat cggtccggct
    30061 gccattacgc tggcgcatcg ttataacgaa gatagccgcg accacggtaa gaaggagcgt
    30121 atggcgcagt tgaacagcca gaacggcgta tggagctgta ctttcgtggg ctattgctcc
    30181 gaagtctgcc cgaaacacgt cgatccggct gcggccattc agcagggcaa agtagaaagt
    30241 tcgaaagact ttcttatcgc gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa
    30301 acgtaaaccg tatgtacggc caatgacgtc cacctggtgg aaaaaattgc cgttttatcg
    30361 cttttacatg ctgcgcgaag gcacggcggt tccggctgtg tggttcagca ttgaactgat
    30421 tttcgggctg tttgccctga aaaatggccc ggaagcctgg gcgggattcg tcgacttttt
    30481 acaaaacccg gttatcgtga tcattaacct gatcactctg gcggcagctc tgctgcacac
    30541 caaaacctgg tttgaactgg caccgaaagc ggccaatatc attgtaaaag acgaaaaaat
    30601 gggaccagag ccaattatca aaagtctctg ggcggtaact gtggttgcca ccatcgtaat
    30661 cctgtttgtt gccctgtact ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt
    30721 ctgacgaacc ggtattctgg ggcctcttcg gggccggtgg tatgtggagc gcaattattg
    30781 cgccggtgat gatcctgctg gtgggtattc tgctgccact ggggctgttt ccgggtgatg
    30841 cgctgagcta cgagcgcgtt ctggcgttcg cgcagagctt cattggtcgc gtattcctgt
    30901 tcctgatgat cgttctgccg ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg
    30961 atctgaaaat tcacgtgcct gcgggcaaat gggttttcta cggtctggct gctatcctga
    31021 cagttgtcac gctgattggt atcgttacaa tctaacgtat cgccaatgta aatccggccc
    31081 gcctatggcg ggccgttttg tatggaaacc agaccttatg ttcaaaacga cgctctgcgc
    31141 cttattaatt accgcctctt gctccacatt tgctgcccct caacaaatca acgatattgt
    31201 gcatcgcaca attaccccgc ttatagagca acaaaagatc cccggtatgg cggtggcggt
    31261 aatttatcag ggtaaacctt attactttac ctggggctat gcggacatcg ccaaaaagca
    31321 gcccgtcaca cagcaaacgt tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt
    31381 gcttggtggc gacgctattg ctcgagggga aatcaagtta agcgatccca caacaaaata
    31441 ctggcctgaa cttaccgcta aacagtggaa tgggatcaca ctattacatc tcgcaaccta
    31501 cactgctggc ggcctgccat tgcaggtgcc ggatgaggtg aaatcctcaa gcgacttgct
    31561 gcgcttctat caaaactggc agcctgcatg ggcgccagga acacaacgtc tgtatgccaa
    31621 ctccagtatc ggtttgttcg gcgcactggc tgtgaagccg tctggtttga gttttgagca
    31681 ggcgatgcaa acacgtgtct tccagccact caaactcaac catacgtgga ttaatgtacc
    31741 gcccgcagaa gaaaagaatt acgcctgggg atatcgcgaa ggcaaggcag ttcatgtttc
    31801 gccaggggcg ttagatgcta aaacttatgg tgtgaagtcg accattgaag atatggcctg
    31861 ctgggtacga agcaatatga atccccgtga tatcaacgac aaaacacttc agcaagggat
    31921 acaactggca caatctcgct actggcaaac cggcgatatg tatcagggcc tgggctggga
    31981 aatgctggac tggccggtaa atcctgacag catcattaac ggcagtggca ataaaattgc
    32041 actggcagca caccccgtaa aagcgattac gcccccaact cctgcagtac gcgcatcatg
    32101 ggtacataaa acaggggcaa ccggcggatt tggtagctat gtcgcgttta ttccagaaaa
    32161 agagctgggt atagtgatgc tggctaacaa aaactatccc aatccagcga gagtcgccgc
    32221 cgcctggcag attctcaacg ctctacagta aaatttcatc gggtccgaat tttcggacct
    32281 tttctgcgct tttccttgct gtcatctaca cttagaaaaa aaccagtaag gaaacattta
    32341 tgcgcctgct ccctctcgtt gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt
    32401 ctcctacgcc gccgcgtggc gtaacagtag tgaataattt cgacgccaaa cgttatctcg
    32461 gtacctggta tgagattgcc cgttttgatc accgttttga acgtggactg gaaaaagtca
    32521 ccgcaacata cagcctgcgt gatgacggcg gcctgaatgt cattaataaa ggctataacc
    32581 ctgacagagg aatgtggcag cagagtgaag ggaaagcgta ctttaccggc gcaccaactc
    32641 gcgctgcgct gaaagtgtca ttctttggtc ctttctatgg cggttataac gttattgcac
    32701 tcgatcggga ataccgccat gcgctggttt gcggcccgga ccgcgactac ctgtggatac
    32761 tctcccgcac gccaaccatt tctgacgaag tgaaacagga gatgctggca gtcgcgaccc
    32821 gggaagggtt tgatgtcagt aagtttattt gggtacagca gcccggtagt tagtgagtgc
    32881 tgagtttcag accaataatc cccaatacga ttaacgccag actcgccagg cgcatcgggt
    32941 tagcggactc accgagcagc acaatgccgg tgatagccgc gccgactgcg ccaatacccg
    33001 tccacacggc ataagccgtc cctactggta acgatttcat cgcccaggca agtagcgcca
    33061 tactgactat catcgccgtc acggtaataa cactcggcgt caaacggcta aagccgtggg
    33121 tatatttcag gccaacggcc cataccactt ccagcagacc agcaataact aagataatcc
    33181 aggacatatc aggctccgga gaattggggc cgtccccggt gaaaagaggc gtttgcaggt
    33241 cgtcctgcaa agctgatggt tgaacggata ttttgccagg tggcagggga atttcaacta
    33301 ttacctttgt gactatcgca aaaaaacaaa cggcacgaca taactgtcgt accgttattg
    33361 cttattgctg cgctttcgtt gctgcaccag aaatcgcgtt accgccatca gaaatgtctt
    33421 caccaacgcc acgcgtggtg ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa
    33481 agatcgctgc aattgtcttc ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt
    33541 ttgcctatag caaattaata atagacaaca tcgccaaaaa tgatggaaat ctgatgattt
    33601 ttcggaagag aagaaaaatt agctggcagc gcgggagatg gagttgccga gatgtttgat
    33661 gtcttcgccg aaaccgcgag cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac
    33721 aagaacgata aggcgtttca tcatctctga cctgaataag tgatggtgca gcctgcaggc
    33781 cgcaccacaa ccgcattact tcacgcgaga gacgtattca ccagagcggg tatccacttt
    33841 gatgacttcg ccgatttgta cgaacagcgg aactttaacc acagcgccag tagacagggt
    33901 agccggtttg ccgccagtac ctgcggtatc acctttcagg cccggatcgg tatcaacgat
    33961 ttccagttca acgaagttcg gcggagtaac ggagatcggc tgaccattcc acagagttac
    34021 gatacactct gcctgatcca gcagccattt agcgttgtcg ccaattgctt ttgcatcagc
    34081 agacagctgc tcgaaagttt cgttgttcat gaagtgccag aactcaccgt cgttgtacag
    34141 gtaagtcagg ttcatatcga caacatcagc gccttcagcg gaatcagtag atttgaaggt
    34201 tttttctacg cgagtaccgg tcagcagacg acgcagttta acgcgagcaa atgcctggcc
    34261 tttacccggt tttacgaatt cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat
    34321 tttaagacca gcacgaaaat cgttgctata gtacgttgcc ataaggccct ctgaaatttg
    34381 ttaattggta gctaagccac aaaatggcgc atattgtaac cctaaatacc ccatccagag
    34441 aagattggtt aacgcaactt gccgatgttg tgaccgatcc tgatgaactt ctgcgtcttt
    34501 tgaatataga cgcggacgaa aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc
    34561 tgcgtgtgcc ccgctcattt atcgatcgca tggagaaagg caatccggac gatcctcttt
    34621 tgcgtcaggt acttacctcg caagatgagt ttgtcgtcgc gcccggattc tccaccgacc
    34681 cactggaaga acagcacagc gtagtgcctg gtttgttgca taaataccac aaccgggcgc
    34741 ttttgctggt caaaggcggc tgcgcggtaa attgccgtta ttgcttccgt cgccacttcc
    34801 cctatgccga aaatcagggc aacaagcgta actggcagac tgctcttgag tatgttgctg
    34861 ctcatccgga actggacgag atgattttct ccggcggcga tccgctgatg gcgaaagatc
    34921 acgagctgga ctggttgctc acacaactgg aagccatccc gcatatcaaa cgtctgcgga
    34981 ttcacagccg tctgccgatt gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct
    35041 ttgcccgttc tacgctgcaa atcttgctgg tgaatcacat caatcatgcc aatgaggtag
    35101 atgaaacgtt ccgtcaggcg atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc
    35161 agagcgttct gttacgtggt gtgaacgata acgcaaaaac gctggcaaac ctgagtaatg
    35221 cgttgttcga tgccggcgta atgccgtatt acctgcatgt gctcgataaa gtacagggcg
    35281 cggcgcattt tatggtgagt gatgacgaag cacggcagat tatgcgtgag ttgctgacac
    35341 tggtgtcggg ttatctggtg ccgaaactgg cgcgagaaat cggcggcgaa cccagcaaaa
    35401 cgccgctgga tctccagcta cgccagcagt aactgattaa acagggaagt taaacattag
    35461 cctgatgcgc tacgcttatc aggcctacat tgtgcattga aattgaatgg atttgcaatg
    35521 aactgtaggc cggataaggc gtttacgccg catccggcaa gagcaacaaa cgcaagtaca
    35581 gcaacctgtc attaaactgg gaaatttaat ctttgcccgt tgcataacct ttaattattc
    35641 atatacattg ctgttgttac tctttatata gccaacacca ataattaacc tttcttttct
    35701 gtttatatag agtgagtacg gctatttatg ttattttaac aactattact accaagccca
    35761 tttatctggc ttataaatta aaattaatta tttaattgag aaatggttag ggagaaccta
    35821 catggccata agcatcaagg gtgttaatac cggtgttatt cgcaagagta ataatttcat
    35881 tgcactggcg ctgaaaatta aagaacctcg caataaagaa tcgttgttct ttatgtctgt
    35941 catggaactg cgcgacctgc tgattgcgct ggaaagtcgt atgcatcaaa agcataaact
    36001 ggatgctgct gcacgccttc agtatgagca agctcgcgat aaagtcatta agaaaatggc
    36061 agaaaacatc ccagaaattc tggttgatga gcttaaaaat gccgatatta atcgtcgggt
    36121 taacacgctg gaacttaccg ataatcaagg cgaaaatctt accttcgtat taacgctaca
    36181 tgacggtagc aaatgtgagt tagtcgtcaa tgagttgcag attgaaatgc tggcgcggca
    36241 atcattcatg caattaataa tgcggaaatg cgtgaactgg cattacgtat tacctcactg
    36301 ttagatttcc tgccgttata tgacgttgac tgccaggaaa atggcaatct ggaatatgac
    36361 acctattccc aaccggaatg gaaacataac ctgttcgacc attatctggc ggtgctttac
    36421 cgctttaaag atgaaagtgg caaagaacaa tttagcggcg cagtggtgaa aacgcgtgaa
    36481 gcaacgccgg gtaaagaaat agaggcgatc actcgccgaa tgcttgattt cagccaacga
    36541 ctgaaaaaac tcgcgggtgt gccatgtcag gtctatgttc gcaccgtagc ggcgaataac
    36601 gcccaaccgc taacccagga tcaatgctta cgcgcgctgc atcacttacg tgttcaatcc
    36661 accagcaaaa ctgcaccaca ggcgaaataa tctcatcttt gctaatgcga ggctggagcc
    36721 atccagcctg aaatgactga taacgtgatc gttggtcatg ccggatgcgg cgtaaacgcc
    36781 ttatccggcc tacaaattct caagcattca atcagttgca atgcctgctg taggcctgat
    36841 aagcgaagcg catcaggcaa tgatacgttt gtcacccagc ctctggatca catcagttcg
    36901 ggcacttata aacctgaccg ataatctggc tatccgtcgg gacaaaactg gacaacattc
    36961 cctgcgacgg gctgctgatg ccataaatca cgttaccgcc cattgcagcc gcctggttgc
    37021 gcagatcgtt tgctgcgccg cgcatagaac cgccctcttc tccgtgttgc ccggaaagcc
    37081 agttgctttg cttacctgtc gcagtaccaa tcagctggca ctctgcgcct ggctgctcgt
    37141 ccacaatgcg tacactctga ccggcagcac tcaattcgtt gctggagcta caacccgcca
    37201 tcagtagcgc ggcaccgaca atccctgcta agtattttac gtgcatgtta ttccccataa
    37261 tcaatgagct ggacgccact cgcgtcgtcc gtgtctgaat cttatactag aaagatgacc
    37321 aaaagaaaaa cccccagaca tttctgcccg ggggtttgtt tatttctgcg aggtgcaggg
    37381 caattacatc atgccgccca tgccacccat gccgcccata ccgccagcag cgcctaagtc
    37441 agctgcatca tttttcggca ggtcggtaac catgcactcg gtggtgatca tcaggccagc
    37501 cacagaagcc gcgtactgca gagcagaacg ggttactttg gttgggtcca ggatacccat
    37561 gtcgatcatg ttgccgtatt cttcggttgc tgcgttgtaa ccgtagttgc cgtcgccgcc
    37621 tttaacggtg ttagcaacaa cagacggttc ttcgccgcag ttcaggacga tctgacgcag
    37681 cggagcttcc attgcacgca gtgcaacttt gatacccacg ttctggtctt cgttctgacc
    37741 acgcaggtca gccagtttag acgctacgcg gatcagcgca acaccaccac cagcaaccac
    37801 gccttcttct accgcagcac gggtcgcgtg cagggcgtct tcaacgcgtg cttttttctc
    37861 tttcatttca acttcggtag cagcacctac tttgataact gcaacgccgc ctgccagttt
    37921 cgctacgcgt tcctgcagtt tttcacggtc gtagtcagaa gttgcttctt caatctgctg
    37981 acggatctga gcaacacggc cctggattgc agcttcttca cccacgccat cgatgatagt
    38041 ggtggtgtct ttgttgatca caacacgttt agcctgaccc aggtcttcca gggttgcttt
    38101 ttccagctcc ataccgatct cttcagagat tacggtaccg ccagtcaggg ttgcgatatc
    38161 ctgcagcata gctttacgac gatcgccgaa gcccggagct ttaaccgcag caactttcac
    38221 gatgccacgc atggtgttaa caaccagagt tgccagcgct tcgccttcaa catcttcagc
    38281 gatgatcagc agcggtttac ctgctttggc aacggcttcc agaaccggca gcatttcgcg
    38341 gatgttggag attttcttgt cagccagcag gatgaacggg ctttccagtt ctactgcgcc
    38401 agtttccggc ttgttgatga agtaaggaga caggtagcca cggtcgaact gcataccttc
    38461 aaccacgtcc agttcgtcct gcagaccggt accgtcttca acggtgataa cgccttcttt
    38521 accgactttg tccatcgctt cagcgatcag tttacctacg gtttcgtcgg agttagcgga
    38581 gatggtacca acctgagcaa tcgctttaga gtcagagcac ggtacggaca gcgctttcag
    38641 ttcttcaact gcagcggtaa cggctttgtc gataccacgt ttcaggtcca tcgggttcat
    38701 gcccgcagca acagctttca gaccctcagt gatgatagcc tgagccagta cagttgcagt
    38761 ggtggtaccg tcgcctgcag cgtcgttcgc tttagaggca acttctttca ccatctgcgc
    38821 acccatattt tcgaacttgt cttccagttc gatttcacga gcaacggaaa caccatcttt
    38881 ggtgatggtc ggtgcaccga aagatttatc cagaactacg ttacggcctt tcggaccgag
    38941 ggtaactttc actgcatctg ccagtacgtt tacgccgcgc agcattttca cacgagcgtc
    39001 gttaccgaat tttacgtctt tagctgccat tatctttatt ccttaaattc gtatgttcag
    39061 tgtcgtgcga ggattacgct tcaacaattg ccagaatgtc gctttcggac atgatcaaca
    39121 cttcttcatt gtcgatcttc tcagatttca caccgtagcc atcgttgaaa ataacgatgt
    39181 cgccaacttt cacatccagc ggcttcactt cgccattttc aaggatacgg ccattgccga
    39241 cagccagcac ttcgccgcgg gtggatttag ccgctgcaga gccggtcaga acgatgccgc
    39301 cagcagattt agtttcaact tctttacgct tgacgatcac gcgatcatgc aatggacgaa
    39361 tattcattga taactctcct ttgagaaagt ccgtatctgt tatgggtgac gccggagctt
    39421 acgtggtttc ccggctggtg accagagaaa tggggatgag gattcgcccc ttcaaggggg
    39481 aaaaagaaaa aaaattctga tcagcacaaa atcgggtgaa aaccctgatt cacctcacat
    39541 ttcatcgcaa tttcttcatc gccgtcataa gcgaatctga ttgtgctacc atcgaaaatc
    39601 tacgcagttg cccaaaattt gggcgcaatc ggatatcaac ccatgagtgg actcaaacaa
    39661 gaactggggc tggcccaggg cattggcctg ctatcgacgt cattattagg cactggcgtg
    39721 tttgccgttc ctgcgttagc tgcgctggta gcgggcaata acagcctgtg ggcgtggccc
    39781 gttttgatta tcttagtgtt cccgattgcg attgtgtttg cgattctggg tcgccactat
    39841 cccagcgcag gcggtgtcgc gcacttcgtc ggtatggcgt ttggttcgcg acttgagcga
    39901 gtcaccggct ggttgttttt atcggtcatt cccgtgggtt tgcctgccgc gctacaaatt
    39961 gccgccgggt tcggccaggc aatgtttggc tggcatagct ggcaactgtt gttggcagaa
    40021 ctcggtacgc tggcgctggt gtggtatatc ggtactcgcg gtgccagttc cagtgctaat
    40081 ctacaaaccg ttattgccgg acttatcgtc gcactgattg tcgctatctg gtgggcgggc
    40141 gatatcaaac ctgcgagtat ccccttcccc gcgccaggaa atatcgaact taccgggtta
    40201 tttgctgcgt tatcagtgat gttctggtgt tttgtcggtc tggaagcatt tgcccatctt
    40261 gcctcggaat ttaaaaatcc agagcgtgat tttcctcgcg cattgatgat tggtctgctg
    40321 ctggcaggat tagtctactg gggctgtacg gtagtcgtct tacacttcga cgcctatggt
    40381 gaacaaatgg cggcggcagc atcgcttcca aaaattgtag tgcaactgtt cggtgtagga
    40441 gcgttatgga ttgcctgcgt aattggctat ctggcctgct ttgccagtct caacatttat
    40501 atacagagct tcgcccgcct ggtctggtcg caggcgcaac ataatcctga ccactacctg
    40561 gcacgcctct cttctcgcca tatcccgaat aatgccctca atgcggtgct cggctgctgc
    40621 gtggtgagca cgttggtgat tcatgcttta gagatcaatc tggacgctct tattatttat
    40681 gccaatggca tctttattat gatttatctg ttatgcatgc tggcaggctg taaattattg
    40741 caaggacgtt atcgactact ggcggtggtt ggcgggctgt tatgcgttct gttactggca
    40801 atggtcggct ggaaaagtct ctatgcgctg atcatgctgg cggggttatg gctgtttctg
    40861 ccaaaacgaa aaacgccgga aaatggcata accacataat ccggcgtttc gacattaatc
    40921 ctggcgatcg tctttatgat caaggcggtc gcgctcatca tcctttcgct ggtactcacc
    40981 atcaaaagta ttaccgccac cggtcccggc gctaaaaccg ccgcctggca tacgagaaaa
    41041 gcgcaaatgc ggcatcaact tcactgtcag atgcttttgc accggcggta ataaaagcag
    41101 aagaccgagg aagtcggtga aaaagcccgg taataaaagc agcaaaccgg caatgatcag
    41161 cgaaacactt ttaatcatct ccgccgctgg gttttcaccc gccgccattt tttgctgcat
    41221 cagcacgaaa ttcttaaagc cctggttacg taccagtgac atcccgataa ctgacgtgaa
    41281 tataaccagc acgagggtca gcaatacccc caatacatgg gcaacctgaa taaagattga
    41341 aatctcaata tagacataaa ggaaaatggc aataaaaggt aaccagcgca aaggtttctc
    41401 ctgtaatagc agccggtaaa acccggctac ctgaatgggt tgcgaatcgc gttcagctta
    41461 tattgtggtc attagcaaaa tttcaagatg tttgcgcaac tatttttggt agtaatccca
    41521 aagcggtgat ctatttcaca aattaataat taaggggtaa aaaccgacac ttaaagtgat
    41581 ccagattacg gtagaaatcc tcaagcagca tatgatctcg ggtattcggt cgatgcaggg
    41641 gataatcgtc ggtcgaaaaa cattcgaaac cacatatatt ctgtgtgttt aaagcaaatc
    41701 attggcagct tgaaaaagaa ggttcacatg tcaaacaaca ttcgtatcga agaagatctg
    41761 ttgggtacca gggaagttcc agctgatgcc tactatggtg ttcacactct gagagcgatt
    41821 gaaaacttct atatcagcaa caacaaaatc agtgatattc ctgaatttgt tcgcggtatg
    41881 gtaatggtta aaaaagccgc agctatggca aacaaagagc tgcaaaccat tcctaaaagt
    41941 gtagcgaatg ccatcattgc cgcatgtgat gaagtcctga acaacggaaa atgcatggat
    42001 cagttcccgg tagacgtcta ccagggcggc gcaggtactt ccgtaaacat gaacaccaac
    42061 gaagtgctgg ccaatatcgg tctggaactg atgggtcacc agaaaggtga atatcagtac
    42121 ctgaacccga acgaccatgt taacaaatgt cagtccacta acgacgccta cccgaccggt
    42181 ttccgtatcg cagtttactc ttccctgatt aagctggtag atgcgattaa ccaactgcgt
    42241 gaaggctttg aacgtaaagc tgtcgaattc caggacatcc tgaaaatggg tcgtacccag
    42301 ctgcaggacg cagtaccgat gaccctcggt caggaattcc gcgctttcag catcctgctg
    42361 aaagaagaag tgaaaaatat ccaacgtacc gctgaactgc tgctggaagt taaccttggc
    42421 gcaacagcaa tcggtactgg tctgaacacg ccgaaagagt actctccgct ggcagtgaaa
    42481 aaactggctg aagtcactgg cttcccatgc gtaccggctg aagacctgat cgaagcgacc
    42541 tctgactgcg gcgcttatgt tatggttcac ggcgcgctga aacgcctggc tgtgaagatg
    42601 tccaaaatct gtaacgacct gcgcttgctc tcttctggcc cacgtgccgg cctgaacgag
    42661 atcaacctgc cggaactgca ggcgggctct tccatcatgc cagctaaagt aaacccggtt
    42721 gttccggaag tggttaacca ggtatgcttc aaagtcatcg gtaacgacac cactgttacc
    42781 atggcagcag aagcaggtca gctgcagttg aacgttatgg agccggtcat tggccaggct
    42841 atgttcgaat ccgttcacat tctgaccaac gcttgctaca acctgctgga aaaatgcatt
    42901 aacggcatca ctgctaacaa agaagtgtgc gaaggttacg tttacaactc tatcggtatt
    42961 gttacttacc tgaacccgtt catcggtcac cacaacggtg acattgtggg taaaatctgt
    43021 gccgaaaccg gtaagagtgt acgtgaagtc gttctggaac gcggtttgtt gactgaagcg
    43081 gaacttgacg atattttctc cgtacagaat ctgatgcacc cggcttacaa agcaaaacgc
    43141 tatactgatg aaagcgaaca gtaatcgtac agggtagtac aaatagagaa ggcacgtcag
    43201 atgacgtgtc ttttttcttg tgagtagtaa cttaaaaata acaatctaat atcaacttgt
    43261 taaaaaacaa ggaaggctaa tatgctagtt gtagaactca tcatagtttt gctggcgatc
    43321 ttcttgggcg ccagattggg gggaataggt attggttttg caggcggatt gggggtgctg
    43381 gttcttgccg ctattggcgt taaacccggt aacatcccgt tcgatgtcat ttccattatc
    43441 atggcggtta tcgccgctat ttctgccatg caggttgctg gcggtctgga ctatctggtt
    43501 catcagacag aaaagctgct gcgccgtaac ccgaaataca tcacgatcct cgcaccgatc
    43561 gtgacctatt tcctgactat ctttgctggt actggcaaca tctctctggc gacactgcca
    43621 gttatcgctg aagttgcgaa ggaacaaggc gttaaacctt gccgtccgct gtctactgca
    43681 gtggtatccg cgcagattgc gatcaccgca tcgccaatct cagcggcagt ggtttacatg
    43741 tcttccgtga tggaaggtca tggcatcagc tacctccatc tgctctccgt ggtcatcccg
    43801 tccaccctgc tggcggttct ggtgatgtcc ttcctggtca ctatgctgtt caactccaaa
    43861 ctctctgacg atccgattta tcgcaagcgt ctggaagagg gcctggttga actgcgcggt
    43921 gaaaagcaga ttgaaatcaa atccggtgca aaaacgtccg tctggctgtt cctgctgggc
    43981 gtagttggcg tggttatcta tgcaatcatc aacagcccaa gcatgggtct ggttgaaaaa
    44041 ccgctgatga acaccaccaa cgcaatcctg atcatcatgc tcagcgttgc aactctgacc
    44101 accgttatct gtaaagtcga taccgacaac atcctcaact ccagcacctt caaagcaggt
    44161 atgagcgcct gtatttgtat cctgggtgtt gcgtggctgg gcgatacttt cgtttccaac
    44221 aacatcgact ggatcaaaga taccgctggt gaagtgattc agggtcatcc gtggctgctg
    44281 gccgtcatct tcttctttgc ttctgctctg ctgtactctc aggctgcaac cgcaaaagca
    44341 ctgatgccga tggctctggc actgaacgtt tctccgctga ccgctgttgc ttctttcgct
    44401 gcggtgtctg gtctgttcat tctgccgacc tacccgacgc tggttgctgc ggtacagatg
    44461 gatgacacgg gtactacccg tatcggtaaa ttcgtcttca accatccgtt cttcatcccg
    44521 ggtactctgg gtgttgccct ggccgtttgc ttcggcttcg tgctgggtag cttcatgctg
    44581 taatgaccaa tcgcggggcg ttcacgcccc gctttctttc ccgccgacta acatccttcc
    44641 cccgtccgtt gtatagtgac ctctctcttg cggttccatc tgttcttgcg aggtgtttat
    44701 gcttgatgaa aaaagttcga ataccgcgtc tgtcgtggtg ctatgtaccg caccggatga
    44761 agcgacagcc caggatttag ccgccaaagt gctggcggaa aaactggcgg cctgcgcgac
    44821 cttgatcccc ggcgctacct ctctctatta ctgggaaggt aagctggagc aagaatacga
    44881 agtgcagatg attttaaaaa ctaccgtatc tcaccagcag gcactgctgg aatgcctgaa
    44941 gtctcatcat ccatatcaaa ccccggaact tctggtttta cctgttacac acggagacac
    45001 agattacctc tcatggctca acgcatcttt acgctgatcc tgctactttg cagcacttcc
    45061 gtttttgccg gattattcga cgcgccggga cgttcacaat ttgtccccgc ggatcaagca
    45121 tttgcttttg attttcagca aaaccaacat gacctgaatc tgacctggca gatcaaagac
    45181 ggttactacc tctaccgtaa acagatccgc attacgtcgg aacacgcgaa aattgccgac
    45241 gtgcagctgc cgcaaggcgt ctggcatgaa gatgagtttt acggcaaaag cgagatttac
    45301 cgcgatcggc tgacgcttcc cgtaaccatc aaccaggcga gtgcgggagc gacgttaact
    45361 gtcacctacc agggctgtgc tgatgccggt ttctgttatc cgccagaaac caaaaccgtt
    45421 ccgttaagcg aagtggtcac caacaacgca gcgtcacagc ctgtgtctgt tccgcagcaa
    45481 gagcagccca ccgcgcaatt gcccttttcc gcgctctggg cgttgttgat cggtattggt
    45541 atcgccttta cgccatgcgt gctgccaatg tacccactga tttctggcat cgtgctgggc
    45601 ggtaaacagc ggctttccac tgccagagca ttgttgctga cctttattta tgtgcagggg
    45661 atggcgctga cttacacggc actgggtctg gtggttgccg ccgcagggtt acagttccag
    45721 gcggcgctac agcacccata cgtgctcatt ggcctcgcta tcgtctttac cttgctggcg
    45781 atgtcaatgt ttggcttgtt tactctacaa ctcccctcct cgctgcaaac gcgcctcacg
    45841 ctgatgagca atcgccaaca gggcggctca cctggcggtg tgtttgttat gggggcgatt
    45901 gccggactga tctgttcacc atgcaccacc gcaccgctta gcgcgattct gctgtatatc
    45961 gcccaaagcg ggaacatgtg gctgggcggc ggcacgcttt atctctatgc gttgggcatg
    46021 ggcctgccgc tgatgctcat taccgtcttt ggtaaccgct tgctgccgaa aagcggcccg
    46081 tggatggaac aagtcaaaac cgcgtttggt tttgtgatcc tcgtactgcc ggtcttcctg
    46141 ctggagcgag tgattggtga tatatgggga ttacgcttgt ggtcggcgct gggtgtcgca
    46201 ttctttggct gggcctttat caccagccta caggctaaac gcggctggat gcgcgtggtg
    46261 caaataatcc tgctggcagc ggcattggtt agcgtgcgcc cacttcagga ttgggcattt
    46321 ggtgcgacgc ataccgcgca aactcagacg catctcaact ttacacaaat caaaacggta
    46381 gatgagttaa atcaggcgct cgttgaagcc aaaggcaaac cggtgatgtt agatctttat
    46441 gccgactggt gcgtcgcctg taaagagttt gagaaataca ccttcagcga cccgcaggtg
    46501 caaaaagcgt tagcagacac ggtcttactt caggccaacg tcacggccaa cgacgcacaa
    46561 gatgtggcgc tgttaaagca tcttaatgtc cttggcctac cgacaattct cttttttgac
    46621 ggacaaggcc aggagcatcc acaagcacgc gtcacgggct ttatggatgc tgaaaccttc
    46681 agcgcacatt tgcgcgatcg ccaaccgtga acgacacttg cagtgggaaa gacggaggaa
    46741 aatagcgtgc aacgtgaaga tgtactggga gaagccctga aattattaga attacaaggg
    46801 attgccaaca ccacgctgga gatgattgct gaacgtgtgg attatccact ggacgagcta
    46861 cgccgcttct ggccagacaa agaggcaatc ctctacgatg cgctgcgtta tcttagccaa
    46921 cagatagatg tctcgcgtcg tcagctgatg ctggacgaaa cgcaaaccgc cgaacaaaag
    46981 ctgctggcac gttatcaggc gttatcggag tgcgttaaaa acaaccgcta tccgggctgt
    47041 ctatttatcg ctgcctgtac gttttatccc gatcctggcc accctattca tcaactggcc
    47101 gatcagcaaa aaagcgcggc ctacgatttc acccacgaac tgttaaccac actggaagtt
    47161 gacgatccgg cgatggtagc aaagcagatg gaactggtgc tggaaggctg tttaagccga
    47221 atgctggtga atcgtagcca ggcggatgtc gacactgcac atcggctggc ggaagatatc
    47281 ctgcgcttcg cccgctgccg ccagggtggt gcattgacct gacagaaaca cagaaaagaa
    47341 gcgatttgcc gcaatcttaa gcagttgaat cgcttttact gaaattaggt tgacgagatg
    47401 tgcagattac ggtttaatgc gccccgttgc ccggatagct cagtcggtag agcaggggat
    47461 tgaaaatccc cgtgtccttg gttcgattcc gagtccgggc accaaattca tatcaacgga
    47521 cctccacgga ggtccgtttt tcgtttcagg acgccacgat ttaagcgttc tgcctccaaa
    47581 tcaattctac cgaactcaac cagattctcc ccacatcacc agcaatttgc gagcatatcc
    47641 caatttggga aaatttgttc ctgagctata gcactaactg acgtgaaatg tcgtgcggcc
    47701 ccgtgatgct gttgaacgtc aaatgacgtc atcaggagcg taacgcatcc ataaagcaca
    47761 acaccggtct gaacgccgac tgatgagatt ttctgaatga gaacaaagag aaatgtatca
    47821 gtccgcttgc tcatgcaaag actaacaatc cattaaaata gtaagcgctc cggacaattt
    47881 tccatggatt attttctgaa catttttctt tggcaaagat gatgaatttt gatggtaagg
    47941 aatattactt ctggttctca gtaaaatcct ttcgtaatac tatgtaatca agaagtttat
    48001 ggctggtaaa aataacgtct tgcattcacc aataacatgt aaataaaccc atctattgat
    48061 ggtaaaaata ggtcatggca attatcattg caccattccc ttttcgaatg agtttctatt
    48121 atgcaacaac ctgtagttcg cgttggcgaa tggcttgtta ctccgtccat aaaccaaatt
    48181 agccgcaatg ggcgtcaact tacccttgag ccgagattaa ttgatcttct ggttttcttt
    48241 gctcaacaca gtggcgaagt acttagcagg gatgaactta tcgataatgt ctggaagaga
    48301 agtattgtca ccaatcatgt tgtgacgcag agtatctcag aactacgtaa gtcattaaaa
    48361 gataatgatg aagatagtcc tgtctatatc gctactgtac caaagcgcgg ctataaatta
    48421 atggtgccgg ttatctggta cagcgaagaa gagggagagg aaataatgct atcttcgcct
    48481 ccccctatac cagaggcggt tcctgccaca gattctccct cccacagtct taacattcaa
    48541 aacaccgcaa cgccacctga acaatcccca gttaaaagca aacgattcac taccttttgg
    48601 gtatggtttt ttttcctgtt gtcgttaggt atctgtgtag cactggtagc gttttcaact
    48661 cttgatacac gtcttcctat gagcaaatcg cgtattttgc tcaatccacg cgatattgac
    48721 attaatatgg taaataaaag ttgtaacagc tggagttccc cgtatcagct ctcttacgcg
    48781 ataggcgtgg gtgatttggt ggcgacatca cttaacacct tctccacctt tatggtgcat
    48841 gacaaaatca actacaacat tgatgaaccg agcagttccg gtaaaacatt atctattgcc
    48901 tttgttaatc agcgccaata ccgtgctcaa caatgcttta tgtcgataaa attggtagac
    48961 aatgcagatg gttcaaccat gctggataaa cgttatgtca tcactaacgg taatcagctg
    49021 gcgattcaaa atgatttact ggagagttta tcaaaagcgt taaaccaacc gtggccacaa
    49081 cgaatgcagg agacgctcca gcaaattttg cctcatcgtg gtgcgttatt aactaatttt
    49141 tatcaggctc atgattattt actgcatggc gatgacaaat cattgaatcg tgccagtgaa
    49201 ttattaggtg agattgttca atcatcccca gaatttacct acgcgagagc agaaaaagca
    49261 ttagttgata tcgtgcgcca ttctcaacat cctttagatg aaaaacaatt agcagcactg
    49321 aacacagaaa tagacaacat tgttacactg ccggaattga ataacctgtc cattatatat
    49381 caaataaaag cggttagtgc tctggtaaaa ggtaaaacag atgagtctta ccaggcgata
    49441 aatactggca ttgatcttga aatgtcctgg ctaaattatg tgttgcttgg caaggtttat
    49501 gaaatgaagg ggatgaaccg ggaagcggct gatgcatatc tcaccgcctt taatttacgc
    49561 ccaggggcaa acacccttta ctggattgaa aatggtatat tccagacttc tgttccttat
    49621 gttgtacctt atctcgacaa atttctcgct tcagaataag taactcccgg gttgatttat
    49681 gctcgggaat atttgttgtt gagtttttgt atgtttctgt tggtataata tgttgcggca
    49741 atttatttgc cgcataattt ttattacata aatttaacca gagaatatcc cgcaatccat
    49801 tgtaaacatt gaatgtttat cttttcatga tatcaacttg cgatcctgat gtgttaataa
    49861 aaaacctcaa gttctcactt acagaaactt ttgtgttatt tcacctaatc tttaggatta
    49921 atcctttttt cgtgagtatt cttagcgcca gtttggtctg gtcaggaaat agttatacat
    49981 catgacccgg actccaaatt caaaaatgaa attaggagaa gagcatgagt tctgccaaga
    50041 agatcgggct atttgcctgt accggtgttg ttgccggtaa tatgatgggg agcggtattg
    50101 cattattacc tgcgaaccta gcaagtatcg gtggtattgc tatctggggt tggattatct
    50161 ctattattgg tgcaatgtcg ctggcgtatg tatatgcccg actggcaaca aaaaacccgc
    50221 aacaaggtgg cccaattgct tatgccggag aaatttcccc tgcatttggt tttcagacag
    50281 gtgttcttta ttaccatgct aactggattg gtaacctggc gattggtatt accgcggtat
    50341 cttatctttc caccttcttc ccagtattaa atgatcctgt tccggcgggt atcgcctgta
    50401 ttgctatcgt ctgggtattt acctttgtaa atatgctcgg cggtacttgg gtaagccgtt
    50461 taaccactat tggtctggtg ctggttctta ttccggtagt aatgaccgct attgttggct
    50521 ggcattggtt tgatgcggca acttatgcag ctaactggaa tactgcggat accactgatg
    50581 gtcatgcgat cattaaaagt attctgctct gcctgtgggc cttcgtgggt gttgaatccg
    50641 cagcagtaag tactggtatg gttaaaaacc cgaaacgtac cgttccgctg gcaaccatgc
    50701 tgggtactgg tttagcaggt attgtttaca tcgctgcgac tcaggtgctt tccggtatgt
    50761 atccatcttc tgtaatggca gcttccggtg ctccgtttgc aatcagtgct tcaaccatcc
    50821 tcggtaactg ggctgcgccg ctggtttctg cattcaccgc ctttgcgtgc ctgacttctc
    50881 tgggctcctg gatgatgttg gtaggccagg caggtgtacg tgccgctaac gacggtaact
    50941 tcccgaaagt ttatggtgaa gtcgacagca acggtattcc gaaaaaaggt ctgctgctgg
    51001 ctgcagtgaa aatgactgcc ctgatgatcc tcatcactct gatgaactct gccggtggta
    51061 aagcatctga cctgttcggt gaactgaccg gtatcgcagt actgctgact atgctgccgt
    51121 acttctactc ttgcgttgac ctgattcgtt ttgaaggcgt taacatccgc aactttgtca
    51181 gcctgatctg ctctgtactg ggttgtgtgt tctgcttcat cgcgctgatg ggcgcaagct
    51241 ccttcgagct ggcaggtacc ttcatcgtca gcctgattat cctgatgttc tacgctcgca
    51301 aaatgcacga gcgccagagc cactcaatgg ataaccacac cgcgtctaac gcacattaat
    51361 taaaagtatt ttccgaggct cctcctttca ttttgtccca tgtgttggga ggggcctttt
    51421 ttacctggag atatgactat gaacgttatt gcaatattga atcacatggg ggtttatttt
    51481 aaagaagaac ccatccgtga acttcatcgc gcgcttgaac gtctgaactt ccagattgtt
    51541 tacccgaacg accgtgacga cttattaaaa ctgatcgaaa acaatgcgcg tctgtgcggc
    51601 gttatttttg actgggataa atataatctc gagctgtgcg aagaaattag caaaatgaac
    51661 gagaacctgc cgttgtacgc gttcgctaat acgtattcca ctctcgatgt aagcctgaat
    51721 gacctgcgtt tacagattag cttctttgaa tatgcgctgg gtgctgctga agatattgct
    51781 aataagatca agcagaccac tgacgaatat atcaacacta ttctgcctcc actgactaaa
    51841 gcactgttta aatatgttcg tgaaggtaaa tatactttct gtactcctgg tcacatgggc
    51901 ggtactgcat tccagaaaag cccggtaggt agcctgttct atgatttctt tggtccgaat
    51961 accatgaaat ctgatatttc catttcagta tctgaactgg gttctctgct ggatcacagt
    52021 ggtccacaca aagaagcaga acagtatatc gctcgcgtct ttaacgcaga ccgcagctac
    52081 atggtgacca acggtacttc cactgcgaac aaaattgttg gcatgtactc tgctccggca
    52141 ggcagcacca ttctgattga ccgtaactgc cacaaatcgc tgacccacct gatgatgatg
    52201 agcgatgtta cgccaatcta tttccgcccg acccgtaacg cttacggtat tcttggtggt
    52261 atcccacaga gtgaattcca gcacgctacc attgctaagc gcgtgaaaga aacaccaaac
    52321 gcaacctggc cggtacatgc tgtaattacc aactctacct atgatggtct gctgtacaac
    52381 accgacttca tcaagaaaac actggatgtg aaatccatcc actttgactc cgcgtgggtg
    52441 ccttacacca acttctcacc gatttacgaa ggtaaatgcg gtatgagcgg tggccgtgta
    52501 gaagggaaag tgatttacga aacccagtcc actcacaaac tgctggcggc gttctctcag
    52561 gcttccatga tccacgttaa aggtgacgta aacgaagaaa cctttaacga agcctacatg
    52621 atgcacacca ccacttctcc gcactacggt atcgtggcgt ccactgaaac cgctgcggcg
    52681 atgatgaaag gtaatgctgg taagcgtctg atcaacggtt ccattgaacg tgcgatcaaa
    52741 ttccgtaaag agatcaaacg tctgagaacg gaatctgatg gctggttctt tgatgtttgg
    52801 cagccggatc atatcgatac gactgaatgc tggccgctgc gttctgacag cacctggcac
    52861 ggcttcaaaa acatcgataa cgagcacatg tatcttgacc cgatcaaagt caccctgctg
    52921 actccgggga tggaaaaaga cggcaccatg agcgactttg gtattccggc cagcatcgtg
    52981 gcgaaatacc tcgacgaaca tggcatcgtt gttgagaaaa ccggtccgta taacctgctg
    53041 ttcctgttca gcatcggtat cgataagacc aaagcactga gcttgctgcg tgctctgact
    53101 gacttcaaac gtgcgttcga cctgaacctg cgtgtgaaaa acatgctgcc gtctctgtat
    53161 cgtgaagatc ctgaattcta tgaaaacatg cgtattcagg aactggctca gaatatccac
    53221 aaactgattg ttcaccacaa tctgccggat ctgatgtatc gcgcatttga agtgctgccg
    53281 acgatggtaa tgactccgta tgctgcgttc cagaaagagc tgcacggtat gaccgaagaa
    53341 gtttacctcg acgaaatggt cggtcgtatt aacgccaata tgatccttcc gtatccgccg
    53401 ggagttcctc tggtaatgcc gggtgaaatg atcaccgaag aaagccgtcc ggttctggag
    53461 ttcctgcaga tgctgtgtga aatcggcgct cactatccgg gctttgaaac tgatattcac
    53521 ggtgcatacc gtcaggctga tggccgctat accgttaagg tattgaaaga agaaagcaaa
    53581 aaataattag ctcgtacaag ggaagtggct tgccacttcc cttttttgct cataaggaga
    53641 acacatgaaa acaccctcac agccgcgcgc gatatactat atcgtggcga tccaaatctg
    53701 ggagtacttc agtttttacg gcatgcgtgc cttactcatt ctctatctca cccatcagct
    53761 tggttttgat gataaccatg ccatcagcct gttcagcgca tatgcttctc tggtttacgt
    53821 tacccctatt ctcggcggct ggcttgccga ccgcctgctc ggcaaccgca ctgcagtgat
    53881 tgccggcgcg ctgttaatga cccttggtca tgtggtgctg ggcattgata caaattcaac
    53941 ctttagcctg tatctggcgc tggcaatcat tatttgtggc tacggtttat tcaaatcaaa
    54001 catcagctgt ttgcttggcg agctttacga cgagaacgat catcgacgtg atggcggttt
    54061 ttcgctgctg tatgctgcgg gcaatatcgg ttctatcgca gccccaatcg cctgcggcct
    54121 ggctgctcag tggtatggat ggcatgttgg ctttgccctt gcgggtggcg gcatgtttat
    54181 cggtttgttg attttcttaa gcggtcatcg tcatttccag tccacgcgta gtatggataa
    54241 aaaagcgctc actagcgtca aatttgcctt accggtatgg agctggttgg tggtgatgct
    54301 ctgtttagcc ccagtatttt ttaccctgct gctggagaac gactggtccg gatatttgct
    54361 ggcgatcgtt tgcctcattg ccgcacaaat cattgcccgc atgatgatca aattccccga
    54421 acaccgccgt gctctttggc aaattgtatt gttgatgttt gtcgggacgt tgttctgggt
    54481 actggcacaa cagggcggca gtaccatcag cttgtttatc gatcgctttg tgaatcgtca
    54541 ggcattcaat attgaagtac ctacagcact attccagtcg gtgaatgcaa ttgcggtgat
    54601 gctcgctggg gttgtactgg cctggctggc gtcgccagaa agccgcggca actcaacatt
    54661 gcgcgtctgg ctgaagtttg cctttggctt actgctgatg gcttgtggct ttatgttgtt
    54721 ggcatttgat gcccgacatg cagcggctga cggtcaagcg tcaatgggcg tgatgatatc
    54781 cgggttggcg ctaatgggct ttgccgaact ctttattgac ccggtggcga ttgcacaaat
    54841 cacgcgtctg aaaatgtctg gcgtattaac cgggatttat atgctggcaa caggcgcggt
    54901 cgccaactgg ctggcaggcg tcgtggcaca gcagacgaca gagtcgcaaa ttagcggtat
    54961 ggcaattgca gcttaccagc gattcttttc tcagatggga gagtggacgt tggcttgtgt
    55021 cgcgatcatc gtggtattgg cctttgctac ccgttttctg tttagcacgc cgacgaatat
    55081 gatacaggag agcaacgatt aactgtcgta aaaacaatag gttatgaata aattaacttt
    55141 cagtttatca cactattaaa tcgagattaa taagtcattt aaaccatttt gatggttatt
    55201 tattagtgat atcaacttga ggtaagcgtt agtttcgata agataaactg agttattaat
    55261 agtcgaggca gataatacag tgtaccgatc tgtctcttta tctacgctaa attgaaagct
    55321 ggatttagag gaaccaaaat gtctgaacaa gaaacacggg gagccaatga ggctattgat
    55381 tttaacgatg aactgagaaa tcgccgcgaa aaactggcgg cactacgtca gcaaggtgtg
    55441 gcgtttccca atgattttcg ccgcgaccat acctctgacc agttgcacga agagtttgat
    55501 gcgaaggata accaggaact ggaatcctta aacattgaag tctcggttgc tggccgaatg
    55561 atgacccgtc gtatcatggg gaaagcctcc tttgtaacgt tgcaggatgt cggtggccgt
    55621 attcaactgt acgtcgcacg agatagcctg ccagaaggtg tttataacga tcagtttaaa
    55681 aaatgggatc tgggtgacat tatcggtgcc cgcggtacgc tgtttaagac gcaaacgggt
    55741 gagctttcca ttcactgtac tgagctgcgc ctgctgacta aagcactacg tcctttacca
    55801 gataaattcc atggtctgca ggatcaggaa gtccgttatc gtcaacgtta tctggacctc
    55861 atcgctaacg ataaatcccg tcaaacgttc gttgtccgtt caaaaattct ggccgctatc
    55921 cgtcagttca tggtcgcgcg cggctttatg gaagtagaaa ccccgatgat gcaggtaatt
    55981 ccaggtgggg catctgctcg cccgtttatt acccatcata atgctctgga tttagatatg
    56041 tatctgcgta tcgcgccaga gctgtatctg aaacgtctgg ttgtaggcgg ttttgaacgg
    56101 gtattcgaaa tcaaccgtaa cttccgtaat gaaggtattt ctgttcgcca taatcctgag
    56161 ttcacaatga tggaactcta catggcgtat gcggattacc acgatttgat tgaactgaca
    56221 gagtccctgt tccgcaccct ggcacaagag gttctgggta ccactaaagt cacttatggc
    56281 gagcatgtgt ttgatttcgg caaaccgttt gaaaaactca ccatgcgcga agcaatcaaa
    56341 aaatatcgtc cagaaaccga tatggccgac ctggataatt ttgatgctgc taaagcatta
    56401 gctgaatcta tcggaattac ggtagagaaa agctgggggc tgggacgtat tgtcacggag
    56461 atctttgatg aagtggcaga agcacatctg attcagccaa cctttattac ggaatatccg
    56521 gcagaagtgt caccgctggc gcgccgtaat gatgttaacc cggaaatcac cgaccgtttt
    56581 gaattcttca tcggtggtcg tgaaatcggt aatggtttta gcgaattaaa cgacgcagaa
    56641 gatcaggctg aacgtttcca ggaacaggtt aacgctaaag ctgcaggtga cgacgaagcc
    56701 atgttctatg acgaagatta cgtgactgcg ctggaatatg gtctgccgcc aaccgctggt
    56761 ctgggtattg gtatcgaccg aatgattatg ctgtttacaa acagtcatac tattcgcgac
    56821 gttattctct tcccggcgat gcgcccacag aaataaattt cactttaatg aacgaagcag
    56881 tcaggcgact gcttcgttcg tcgttccgca aatccagttt tgaggagaga cgcttcgttt
    56941 cacgataacc agcgggtcca ctcaactaca gctcctaaaa gagagaaaaa agtaatgcca
    57001 caggcagact cattggccag gttattccga ccaggaatgc actaagtaaa cgaatatgtg
    57061 ttttctcatg tgagataaac
//
LOCUS       DAZGVQ010000009        55231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000009 DAZGVQ010000000
VERSION     DAZGVQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.009"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            14..565
                     /gene="ygiL"
                     /locus_tag="ACHLAG_000517"
     CDS             14..565
                     /gene="ygiL"
                     /locus_tag="ACHLAG_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFP0653819.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
     gene            625..3129
                     /locus_tag="ACHLAG_000518"
     CDS             625..3129
                     /locus_tag="ACHLAG_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0653820.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEVAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSFSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3145..3894
                     /gene="yqiH"
                     /locus_tag="ACHLAG_000519"
     CDS             3145..3894
                     /gene="yqiH"
                     /locus_tag="ACHLAG_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0653821.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3896..4960
                     /gene="yqiI"
                     /locus_tag="ACHLAG_000520"
     CDS             3896..4960
                     /gene="yqiI"
                     /locus_tag="ACHLAG_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0653822.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNMVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(5003..5203)
                     /gene="glgS"
                     /locus_tag="ACHLAG_000521"
     CDS             complement(5003..5203)
                     /gene="glgS"
                     /locus_tag="ACHLAG_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFP0653823.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            5472..6101
                     /gene="yqiJ"
                     /locus_tag="ACHLAG_000522"
     CDS             5472..6101
                     /gene="yqiJ"
                     /locus_tag="ACHLAG_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFP0653824.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            6128..7789
                     /gene="yqiK"
                     /locus_tag="ACHLAG_000523"
     CDS             6128..7789
                     /gene="yqiK"
                     /locus_tag="ACHLAG_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP0653825.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            8028..8087
                     /locus_tag="ACHLAG_000524"
     CDS             8028..8087
                     /locus_tag="ACHLAG_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0653826.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            8404..8463
                     /gene="ibsE"
                     /locus_tag="ACHLAG_000525"
     CDS             8404..8463
                     /gene="ibsE"
                     /locus_tag="ACHLAG_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFP0653827.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(8583..10016)
                     /gene="hldE"
                     /locus_tag="ACHLAG_000526"
     CDS             complement(8583..10016)
                     /gene="hldE"
                     /locus_tag="ACHLAG_000526"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFP0653828.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(10064..12904)
                     /gene="glnE"
                     /locus_tag="ACHLAG_000527"
     CDS             complement(10064..12904)
                     /gene="glnE"
                     /locus_tag="ACHLAG_000527"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFP0653829.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFRAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(12927..14228)
                     /gene="ygiF"
                     /locus_tag="ACHLAG_000528"
     CDS             complement(12927..14228)
                     /gene="ygiF"
                     /locus_tag="ACHLAG_000528"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFP0653830.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHALMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            14470..15090
                     /gene="ygiM"
                     /locus_tag="ACHLAG_000529"
     CDS             14470..15090
                     /gene="ygiM"
                     /locus_tag="ACHLAG_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFP0653831.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            15154..16392
                     /gene="cca"
                     /locus_tag="ACHLAG_000530"
     CDS             15154..16392
                     /gene="cca"
                     /locus_tag="ACHLAG_000530"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFP0653832.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(16555..17376)
                     /gene="bacA"
                     /locus_tag="ACHLAG_000531"
     CDS             complement(16555..17376)
                     /gene="bacA"
                     /locus_tag="ACHLAG_000531"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP0653833.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMLWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(17466..17834)
                     /gene="folB"
                     /locus_tag="ACHLAG_000532"
     CDS             complement(17466..17834)
                     /gene="folB"
                     /locus_tag="ACHLAG_000532"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFP0653834.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            17939..18556
                     /gene="plsY"
                     /locus_tag="ACHLAG_000533"
     CDS             17939..18556
                     /gene="plsY"
                     /locus_tag="ACHLAG_000533"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFP0653835.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(18569..19501)
                     /gene="ttdR"
                     /locus_tag="ACHLAG_000534"
                     /gene_synonym="dan"
     CDS             complement(18569..19501)
                     /gene="ttdR"
                     /locus_tag="ACHLAG_000534"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFP0653836.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            19708..20619
                     /gene="ttdA"
                     /locus_tag="ACHLAG_000535"
     CDS             19708..20619
                     /gene="ttdA"
                     /locus_tag="ACHLAG_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFP0653837.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            20616..21221
                     /gene="ttdB"
                     /locus_tag="ACHLAG_000536"
     CDS             20616..21221
                     /gene="ttdB"
                     /locus_tag="ACHLAG_000536"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFP0653838.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            21269..22732
                     /gene="ttdT"
                     /locus_tag="ACHLAG_000537"
     CDS             21269..22732
                     /gene="ttdT"
                     /locus_tag="ACHLAG_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFP0653839.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTLYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(22775..23788)
                     /gene="tsaD"
                     /locus_tag="ACHLAG_000538"
     CDS             complement(22775..23788)
                     /gene="tsaD"
                     /locus_tag="ACHLAG_000538"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFP0653840.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            24026..24241
                     /gene="rpsU"
                     /locus_tag="ACHLAG_000539"
     CDS             24026..24241
                     /gene="rpsU"
                     /locus_tag="ACHLAG_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFP0653841.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            24352..26097
                     /gene="dnaG"
                     /locus_tag="ACHLAG_000540"
     CDS             24352..26097
                     /gene="dnaG"
                     /locus_tag="ACHLAG_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP0653842.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQKPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            26292..28133
                     /gene="rpoD"
                     /locus_tag="ACHLAG_000541"
     CDS             26292..28133
                     /gene="rpoD"
                     /locus_tag="ACHLAG_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFP0653843.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSNAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(28211..28717)
                     /gene="mug"
                     /locus_tag="ACHLAG_000542"
     CDS             complement(28211..28717)
                     /gene="mug"
                     /locus_tag="ACHLAG_000542"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFP0653844.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            28842..28917
                     /locus_tag="ACHLAG_000543"
     tRNA            28842..28917
                     /locus_tag="ACHLAG_000543"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28875..28877,aa:Ile,seq:cat)
     gene            29116..30381
                     /locus_tag="ACHLAG_000544"
     CDS             29116..30381
                     /locus_tag="ACHLAG_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001384119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFP0653845.1"
                     /translation="MTKKVKSLSPATIKSLQRKASNEKKDLFITDGEGLELVIKHKSA
                     SCLWRYRYYKPHTKKRAMLSIGAYPEISIAGARKIKDEYRALLSGGIDPKEHREKQRL
                     SEVMKRENTFRHVATLWFELKKGQGLAEDTLRDIWRSLERNVFPSVGNRPIADLIASN
                     FIEALKPLAAQGKLDTVRRTAQRINEVMYYAVNTGMIAANPASEISKAFNAPSVKNMP
                     AIRPEELPEFMQKLATARVERQTRLVIEWQLLTICRPGEAAGARWCEIDFEVKEWRIP
                     EHRLKQRREHVIPLSKQALAILDVMKPISGHREFIFPHNSDPQRHMCVQTANAAIKRM
                     GFSGRLVAHGLRSIASTALNEQGFDSDVIEAALSHIDKNEVRRAYNRSTYLEQRRPLM
                     AWWGDFVEAAASGSVSATGGYRGLKMVNG"
     gene            30386..31282
                     /locus_tag="ACHLAG_000545"
     CDS             30386..31282
                     /locus_tag="ACHLAG_000545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0653846.1"
                     /translation="MANKKINLPERDYYPLDKAARRLECDVDDLLHFIATRRMRFLYR
                     ININEKLTLKYYEGVKPELISANKTLYQHYTNEYCNVIIDATSINKLLTPIDVFYYAH
                     AGLNINYHYSSYGDYFSMNIFAEDEPEIKNAQIITPAKEIYVKADGLLNVKANSADIE
                     RLSFHDTTNTKYIQIIDNDDEDDFKAAILLQGWDISRNDIFISRSEIDLAINGGRTAS
                     IENMDELNINKPPPSKKTTNLQARVIKRLITAIAGEEEASNPRRALENGHSKLSRALT
                     KAGVDIGATPTCIGKWLADVDD"
     gene            31420..31623
                     /locus_tag="ACHLAG_000546"
     CDS             31420..31623
                     /locus_tag="ACHLAG_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0653847.1"
                     /translation="MNITEKKILTKREVANLLRLSTTSIDTMVKAGEFIPGFKRGLRR
                     VGWFEDEVKSWLEKKVEERYQQE"
     gene            31797..32123
                     /locus_tag="ACHLAG_000547"
                     /pseudo
     CDS             31797..32123
                     /locus_tag="ACHLAG_000547"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            32154..32342
                     /locus_tag="ACHLAG_000548"
     CDS             32154..32342
                     /locus_tag="ACHLAG_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0653848.1"
                     /translation="MATTPALVHSQTAFIWRFITFGTSERQIIHVTAWTEREARNRCP
                     SGCVAVFAARIRQGVSHA"
     gene            32335..32799
                     /locus_tag="ACHLAG_000549"
     CDS             32335..32799
                     /locus_tag="ACHLAG_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000916858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0653849.1"
                     /translation="MLKNMLIASSFGLYDAGVVHMATGFSSLNTKADSRLHSDMRFFC
                     VRKPTHTRIMAGRNGGAFALAGFFGSSLLTLLRLATPFSSVVARLLIKTKGAFHMAVF
                     ARPYFVWRFMQCLTGSIAIFTVTAATEHEARAQLPHTHLIFVTLIREGVSYA"
     gene            32792..33106
                     /locus_tag="ACHLAG_000550"
     CDS             32792..33106
                     /locus_tag="ACHLAG_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0653850.1"
                     /translation="MRDVCNTRILEQLVTTIKAADAREARAMFDDADFYARKLLEGLA
                     ITGRLLSGMSDSIDPDMDELRSLGDSIAVTAELVAGFSEVVEVYNWRCRNGDISADYR
                     HS"
     gene            33180..33479
                     /locus_tag="ACHLAG_000551"
     CDS             33180..33479
                     /locus_tag="ACHLAG_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0653851.1"
                     /translation="MNMKKTGLTASGKPRPEFKRGDIYRDQYGGTVMIKGVAGRCVTY
                     RRKGYEYDCVMPVYQFSRDFSLVQAVPRNVPTSNVKALAKIQKMKKMINAFRGKK"
     gene            33476..34873
                     /locus_tag="ACHLAG_000552"
     CDS             33476..34873
                     /locus_tag="ACHLAG_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFP0653852.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQEGRLAGDN
                     VPPVWLGEQQLAELADTKIIDEGRYCVRLYKAGHIRPSNINAIAQKLAAAGVQDANYY
                     PDGMHSQKRENWREYLERERGEKQEEKGHQRKTKLPMSVGSAGYDTQLDYVVKGIIPA
                     VSLCSIYGASGSYKSFLAGSWACHVSTGRQWGGRRVAHGAVLYVVGEGGIGVPRRVKA
                     WEVVHGEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCFG
                     GNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYRI
                     RREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDKPRHPVE
                     PERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKRLGYDVKNMRRWLAKLE
                     KDDVIYIDGDDVGPL"
     gene            35314..35724
                     /locus_tag="ACHLAG_000553"
     CDS             35314..35724
                     /locus_tag="ACHLAG_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0653853.1"
                     /translation="MTAQIAAYGRLIDDPQTKQTSKGTPMTLARMAVSLPRSQAQDGQ
                     ATLWLSVLAFGKQADQLAGHEKGNVISVSGVMQVSQWTGQNGETRQGYQVIADSVISA
                     KTARPGGNRRRTTGTQGNQPPAGGDDPYGDNIPF"
     gene            35735..36004
                     /locus_tag="ACHLAG_000554"
     CDS             35735..36004
                     /locus_tag="ACHLAG_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0653854.1"
                     /translation="MVHDSIAEELEAKGFYRRAAARWAEVMMLVETDRERERVAMRRA
                     ECYGKTQRPPEQPDDLGGLKRAIKRTEKEMGIDGRGKKIWRSYQK"
     gene            36118..36525
                     /locus_tag="ACHLAG_000555"
     CDS             36118..36525
                     /locus_tag="ACHLAG_000555"
                     /inference="COORDINATES: protein motif:HMM:NF020345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFP0653855.1"
                     /translation="MNEQTHHVPKDIERLKFIDAIDNGDKPGDAWCDSLADIVSHSVA
                     ELTRNGMEEADAFRIAKRVVAAIAFYAGGRSLYLPTGRSLFAALREYEIYYRWDNGEK
                     LQALRKEYAISETHIYDIIRRLRKKHANKITQD"
     gene            36912..37002
                     /locus_tag="ACHLAG_000556"
     tRNA            36912..37002
                     /locus_tag="ACHLAG_000556"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            37026..37107
                     /locus_tag="ACHLAG_000557"
     tRNA            37026..37107
                     /locus_tag="ACHLAG_000557"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37064..37066,aa:Tyr,seq:gta)
     gene            complement(37418..38182)
                     /gene="nfeF"
                     /locus_tag="ACHLAG_000558"
                     /gene_synonym="yqjH"
     CDS             complement(37418..38182)
                     /gene="nfeF"
                     /locus_tag="ACHLAG_000558"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFP0653856.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            38459..39082
                     /gene="nfeR"
                     /locus_tag="ACHLAG_000559"
                     /gene_synonym="yqjI"
     CDS             38459..39082
                     /gene="nfeR"
                     /locus_tag="ACHLAG_000559"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFP0653857.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(39236..40729)
                     /gene="aer"
                     /locus_tag="ACHLAG_000560"
     CDS             complement(39236..40729)
                     /gene="aer"
                     /locus_tag="ACHLAG_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFP0653858.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASAADKLSITASNAAVQGGEAMTTVIKTMDD
                     IADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGEVRHLASRSA
                     NAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQISHSTLEQADG
                     LSSLTRAVDELNLITQKNAELVEESAQVSEMVKHRASRLEDAVTVLH"
     gene            41147..42526
                     /gene="ygjG"
                     /locus_tag="ACHLAG_000561"
     CDS             41147..42526
                     /gene="ygjG"
                     /locus_tag="ACHLAG_000561"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFP0653859.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(42568..42900)
                     /gene="ygjH"
                     /locus_tag="ACHLAG_000562"
     CDS             complement(42568..42900)
                     /gene="ygjH"
                     /locus_tag="ACHLAG_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFP0653860.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            43119..44102
                     /gene="ebgR"
                     /locus_tag="ACHLAG_000563"
     CDS             43119..44102
                     /gene="ebgR"
                     /locus_tag="ACHLAG_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFP0653861.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            44286..47378
                     /gene="ebgA"
                     /locus_tag="ACHLAG_000564"
     CDS             44286..47378
                     /gene="ebgA"
                     /locus_tag="ACHLAG_000564"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFP0653862.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTHGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            47375..47824
                     /gene="ebgC"
                     /locus_tag="ACHLAG_000565"
     CDS             47375..47824
                     /gene="ebgC"
                     /locus_tag="ACHLAG_000565"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFP0653863.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            47887..49320
                     /gene="ygjI"
                     /locus_tag="ACHLAG_000566"
     CDS             47887..49320
                     /gene="ygjI"
                     /locus_tag="ACHLAG_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0653864.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            49454..50524
                     /gene="ygjJ"
                     /locus_tag="ACHLAG_000567"
     CDS             49454..50524
                     /gene="ygjJ"
                     /locus_tag="ACHLAG_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFP0653865.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            50541..52892
                     /gene="ygjK"
                     /locus_tag="ACHLAG_000568"
     CDS             50541..52892
                     /gene="ygjK"
                     /locus_tag="ACHLAG_000568"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP0653866.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            53218..>55231
                     /gene="fadH"
                     /locus_tag="ACHLAG_000569"
     CDS             53218..>55231
                     /gene="fadH"
                     /locus_tag="ACHLAG_000569"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFP0653867.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVFAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALE"
BASE COUNT        14188 a        14273 c        13936 g        12834 t
ORIGIN      
        1 ataaaggaat attatgtctg cttttaaaaa gtcacttctt gttgctggcg tagcaatgat
       61 tctttccaat aacgtatttg ctgacgaagg tcacggcatt gttaaattta aaggtgaagt
      121 tatctctgct ccatgttcaa ttaaaccagg tgacgaagac ttaactgtaa atctgggtga
      181 agtagcagat accgttctga aaagcgacca gaaatctttg gctgagcctt tcactattca
      241 cctgcaagac tgcatgttga gccagggtgg aaccacttat tcaaaagcga aggttacttt
      301 caccacagcg aatactatga cgggtcagac tgacctgctg aaaaacacca aagaaactga
      361 aatcggtggt gccacaggcg ttggcgtacg tattctggat agccagagcg gcgaagtgac
      421 tttaggtact ccggtagtaa ttacattcaa caatactaac agctatcagg aactgaactt
      481 caaagctcgt atggaatccc caagcaaaga tgctaccccg ggcaacgttt acgctcaggc
      541 tgactataag attgcttacg agtaatcaca ttaggccggg ttcgcccggc cacatcatta
      601 acggattaat gataagtgga acagatgtat aaaaaattaa aattaaccac aataagcgaa
      661 ttgattaaaa atatttattg ttcattatcc gttctcatca ttggttgtgc gtcagcttat
      721 gccgttgaat tcaacaaaga tttaatcgaa gccgaagatc gtgaaaacgt taacctttcc
      781 caatttgaaa ctgatggcca attacccgta ggtaaatact cattaaacgc tcttattaat
      841 aataaaagga cgccaatcca tctggacctg caatgggtat taattgataa ccaaactgca
      901 gtttgtctga ctccagagca attaacatta ttaggcttta ctgatgagat tattgaagtg
      961 gctcagcaaa acctgatcga tggttgttac cctatcgaaa aagaaaaaca aattacaact
     1021 tatctcgata aagggaaaat gcaattatcc atatctgcac ctcaggcatg gttaaaatac
     1081 aaagatgcaa actggacgcc tcctgaactt tgggatcatg gtattgctgg ggcatttctt
     1141 gactacaatt tatatgcctc tcattatgca ccacatcagg gcgataattc acaaaatata
     1201 agttcctatg ggcaggctgg ggttaatctt ggggcctggc gcctgcgtac tgattaccag
     1261 tacgatcaat catttaacaa tggcaaaagc caggcgaaca acctggattt tccgcgtatt
     1321 tatttgtttc gcccaatccc agcaattaat gcaaaactaa ctataggtca atacgatact
     1381 gaatcctcta ttttcgactc tttccatttt tctggcgttt cgttgaaaag cgatgagaat
     1441 atgttgccgc ctgatctacg tggttacgca ccgcaaatca cgggtgttgc acaaacgaat
     1501 gcaaaggtca ctgtctcaca gaacaaccgt attatttatc aagaaaatgt tcctcctggc
     1561 ccatttgcta ttactaattt attcaataca ttacaggggc aactggacgt caaggttgaa
     1621 gaagaggacg ggcaagtcac gcaatggcaa gttgcatcta atagtattcc ttatctgacg
     1681 cgtaaagggc agattcgcta caccactgct atgggtaaac cgaccagcgt tggtggtgat
     1741 tccttacaac aacccttctt ctggactggt gaattctcat ggggttggct gaacaatgta
     1801 tccctgtatg gtggttcagt tttaacaaac cgtgattatc aatctctggc tgccggcgtt
     1861 ggctttaacc ttaactcatt gggttcatta tcttttgatg tcacacgatc tgatgctcag
     1921 ttgcataatc aggataaaga aacgggttat agctaccgcg ctaactattc aaaacgtttt
     1981 gaatctaccg gtagccagct cactttcgct ggttaccgtt tctctgataa aaactttgtg
     2041 acaatgaatg aatatatcaa tgacactaac cattacacga attatcagaa tgaaaaagag
     2101 agttatattg tcacgtttaa ccagtatctt gaatcattaa ggttaaatac atacgtaagt
     2161 ttggctcgta atacttactg ggacgccagc agtaatgtga attattcatt ttcacttagc
     2221 cgcgattttg atatcgggcc attaaaaaac gtctccactt ctctaacatt tagccgaata
     2281 aactgggaag aagacaacca ggatcaactg tacctaaata tttcgattcc ctggggaacc
     2341 agtagaacat tgagctatgg tatgcaacga aatcaggata acaagatttc gcatactgct
     2401 tcgtggtatg actcttccga tcgaaataat tcctggagcg tttctgcttc aggcgacaat
     2461 gatgaattca aagatatgaa agcgtcacta cgcgccagtt atcagcataa taccgagaac
     2521 ggtcgtctct atctctccgg gacatcacag cgagacagtt attattctct gaatgccagt
     2581 tggaatggtt cattcacggc gactcgtcac ggagccgctt tccatgacta tagtggtagt
     2641 gctgactcgc gttttatgat cgacgcagat ggtgcagaag atattccgtt gaacaataaa
     2701 cgcgcggtaa ctaatcggta tggcatcgga gttattccat cagtcagcag ttacataaca
     2761 acatcattaa gcgttgatac ccgaaatctt ccagaaaatg tggatatcga aaactcggtt
     2821 atcaccacca ccttaaccga aggtgctatt ggctacgcca aacttgatac ccgcaagggc
     2881 tatcaaatca tgggggttat tcgcctggca gatggtagtc acccaccact ggggattagc
     2941 gtaaaagata aaaccagcca taaagaatta ggactggttg ctgatggcgg ctttgtatac
     3001 ctcaacggca ttcaggatga cagcaaactt actttacgct ggggtgataa atcctgtttt
     3061 attcaaccac ccaatagcag caacttaacc accggaacgg ttattttacc gtgtattagc
     3121 caaaattaat tcaggatgaa actaatgcgc tacttaaaca caaagaatct cattgcagca
     3181 ggagtattac tcgcctgcat gagcagcatt gcctggggtg ccatcatccc ggaccgtacc
     3241 cgtatcataa tgaatgagtc agataaaggg gaagcattaa aacttactaa tcaaagcaaa
     3301 aatctaccct atctggcgca aacatggata gaagatacga aaggcaataa atcacgtgac
     3361 tttattgtca cggttccccc tatggtacgt ttaaatccca gtgagcaaat tcaaataaga
     3421 atgataagtc aggaaaaaat tgctcaactg cctaatgaca gagaaacgct cttctatttt
     3481 aacgtgcgag aaataccgcc aaaaacagat aaaaaaaatg tgatgcaagt cactatgcag
     3541 cacgcattga aactattctg gcgaccaaaa gctattgaat tagaagatga tggggtaatg
     3601 tcttacgaaa aagttgaaat catccgtaga aatgatggtt cgatccgctt taacaataag
     3661 atgccttatc acgttacgct gggttatatc ggaacaaacg gaatcacaat gttgccacaa
     3721 acgcaaagtt taatggtaac cccttatagc catgcagata cgcagtttaa aaatgttccg
     3781 tcagcttttc aggttggtta tatcaatgat tttggtggat taagttttta tgaaataaac
     3841 tgcccgacgg ttaataactc ttgtaatgtt tccgtagcca aacgagataa ataatatgcg
     3901 ctacttatta attgtaatta tatttattat aggttttagt gcattacctg tatgggcaat
     3961 ggactgctat gctgaacatg aagggggaaa taccgtagtc attggttacg taccaagaat
     4021 ttctatcccc agtaatggta aaaaaggtga taagatctgg caaagcagtg agtattttat
     4081 gaatgtcttc tgtaataatg cattacctgc cccatcacct ggagaagaat acccatcagc
     4141 ctggactaat ataatgatgt ttttaccagg cggtcaggac ttttataatc agaactccta
     4201 tatttttggc gtaacataca atggagttga ttatgattcg actgccccta atgctatcgc
     4261 agcaccagaa tgcattgata taaaaggagc gggtaccttt aataatcact ataaaaaccc
     4321 cgctgtctgt agtggaggtc ctgagcctca attatcagta acctttccag ctcgcgtaca
     4381 actctatatt aaattagcaa aaaatgccaa tagagttaat aaaaacatgg tgttacctga
     4441 tgaatatatc gctctagagt ttaaaggtat gagtggcgca ggagctatag aagtagacaa
     4501 aaatttgacc ttcaggattc gtggattaaa taatattcat gtccttgact gctttgttaa
     4561 tgtcgcactg gaaccagctg atggcgttgt tgactttggt aaaataaatt cccgaacaat
     4621 taaaaatacc agcgttagtg agacgtttag tgtagtcatg acaaaagatc ctggtgcggc
     4681 ctgtactgag caattcaata ttttagggag ctttttcact acggatattt tgagtgatta
     4741 tagccatctg gatatgggta atggactgct attgaagata tttcataaag atggaacagc
     4801 aacggaattt aaccgcttct cacaatttgc ttctttttca tcgtctagtg caccttcggt
     4861 caccgcacca ttcagggcag aacttagtgc gaatccggca gagactgttg ttgagggacc
     4921 gtttagtaaa gacgtcatcc tcaaaatcac ctataactag catctaatac aaacactaaa
     4981 acgggccatc aggcccgtta tatcagtgct ctaactccag ctctcttgcc tgcatcatct
     5041 gttgacaata ccaggcataa cgtgcgcaaa accaggttct atgttcttca tccatgttac
     5101 cagtaacggc aagaatatcg accgggcgac cttctgcgtg cattctggca aaactacgcg
     5161 ccaggaaatc gaaattattt aaagaattaa gactatgatc cataagcaca tcctccggtt
     5221 atcgcatacc ttttaaggcc atatgctttt tctgatttca gtattcccca atcagtgtag
     5281 tgcctttaaa cataacgtgc gtaaatatat tgtccccgat cacacttttt agtgaaaaat
     5341 aaaaaaccat attaaaaaac tttctcttcc agaacttctc taacatgtgt tattattaac
     5401 aaagataaat gtaagcaagg aaactatccc aaagtaaata ttgcgtcact aaatggacat
     5461 tggagtgaca tatgatttta ttcgccgact ataatacccc ttatcttttc gctatttcct
     5521 ttgtactgtt gatcggcctt ttagaaattt tagcgcttat ctgcggtcac atgctctccg
     5581 gtgctcttga tgcacatctt gaccattatg attccataac cacaggtcat attagccagg
     5641 cacttcatta tctcaatatc ggaagattgc cagctctcgt cgttctctgc ttgctggctg
     5701 gtttttttgg tcttattggc atcctgttgc agcatgcctg catcatggtc tggcaatcac
     5761 cactgtcgaa cctgttcgtg gttcctgtca gtttactgtt tacgattatt gcggtgcatt
     5821 ataccggtaa aatagttgcg ccctggattc cacgcgacca tagttcagct attacagaag
     5881 aagaatatat tggtagcatg gcattaatta ccggtcatca agccacttca ggcaatccct
     5941 gcgaaggaaa actcaccgat caatttggcc aaatacatta tttactactg gagcctgaag
     6001 aaggaaaatt tttcactaaa ggagacaaag tgttaattat ttgccgactt tctgcaacgc
     6061 gatatttggc ggaaaacaat ccctggccac aaatattgta atttattaat caaaaggaaa
     6121 tttaataatg gatgatattg ttaattctgt gccctcctgg atgtttaccg cgattattgc
     6181 cgtatgcatt ctgtttatta ttggaattat tttcgccagg ctctatcgtc gcgcttcggc
     6241 agagcaagct tttgttcgta ctggtttagg tgggcaaaaa gtggtgatga gcggtggcgc
     6301 aatcgtgatg ccgatctttc atgaaataat ccccatcaat atgaatactc tgaagctgga
     6361 agtcagccgc tcaaccattg atagcctgat tacgaaagat cgtatgcgtg tcgatgtagt
     6421 agtcgctttc tttgtacggg taaaaccgtc tgtagaaggc atcgccactg cggcgcagac
     6481 gttggggcaa cgcacgctat caccagaaga cttacgtatg ttggttgaag ataaatttgt
     6541 cgatgccctc cgtgcaacag ctgcacaaat gaccatgcat gagttacagg atacccgcga
     6601 gaacttcgtg cagggagtac aaaatactgt cgcagaagac ctgtcgaaaa acggtcttga
     6661 gctggaaagc gtttcactta ccaactttaa ccagacctcg aaagaacatt tcaatccgaa
     6721 caatgccttt gacgccgaag gtttaaccaa actgactcag gagacggagc gccgtcgtcg
     6781 cgaacgtaac gaagttgaac aggatgtaga agttgcggtg cgtgagaaaa accgtgatgc
     6841 actttcgcgc aagttggaga ttgaacaaca agaagcgttt atgacgcttg agcaggagca
     6901 gcaggttaaa acccgtactg ccgaacagaa tgcacgtatt gcggcttttg aagctgaacg
     6961 tcgtcgtgaa gcagagcaga cgcgaattct ggctgaacga caaattcagg aaacggagat
     7021 cgagcgcgag caggccgtcc gctcaagaaa ggttgaggct gaacgtgaag ttcgtattaa
     7081 agagatcgaa caacagcagg tcaccgaaat cgccaaccag acgaaatcga tcgctattgc
     7141 cgccaaatcg gaacaacagt cccaggcaga agcgcgtgct aaccttgcac tcgcagaagc
     7201 agtaagtgcg caacaaaacg tagaaaccac tcgccagacc gcagaagccg atcgtgctaa
     7261 acaagttgcc ctaatcgctg ccgcgcagga tgcagaaacc aaagcggttg aactgaccgt
     7321 gcgggcgaaa gcagaaaaag aagccgcaga gatgcaggcg gcggctatcg ttgagttagc
     7381 cgaagctaca cgtaaaaagg gtctggcgga agcagaagca caacgtgcgc tgaacgatgc
     7441 tatcaacgta ctttctgatg aacaaaccag ccttaaattc aaactggcct tgttgcaggc
     7501 gctacctgcg gtaatagaaa aatccgttga gccgatgaaa tctattgacg gtatcaagat
     7561 tattcaggtc gatggtctga atcgtggcag cgctgcgggt gatgcaaaca cgggtaatgt
     7621 ggggggcgga aacctggcgg aacaagcatt atcagccgct ctctcttacc gcacacaggc
     7681 accgctgatt gactccttgc tcaatgaaat tggcgtttca ggcggctcac tggcggcatt
     7741 gacttcaccc ttaacctcaa caactcccgt cgccgaaaac gtagaataaa ttttcagcta
     7801 tgaacgtccc tggcacaaaa agccggggat tttttatatc tgcgttccgc taaaaactgc
     7861 aaatgatcag gtcgttgcaa cgttttgcgt gaccgctcgg ggaaggcaaa actgcctctg
     7921 ggaaagcgtt gcgcgaggtc cggcgctcat caacaatcgg ggagtcagca aagggctgaa
     7981 acgggaaagc ccctcccgag gaaggggcct tacagaagga aagggttatg atgaagctcg
     8041 tcatcatact gattgtgttg ttaatcgtaa gtttcgcagc ttattaacag ccaatcagag
     8101 gggggagaaa tcctccctca cccttgttcc tttactttag gttgaaaaaa aatcagcgtc
     8161 aagaaggttt cattaacaca tatccctggc ccaaaaagcc ggggattttt tatatctgcg
     8221 ttccgctaaa aggtgcaaat gctcaggacg ttgcagcgtt ttgcgtgacc gctcggggaa
     8281 ggcaaaactg cttcagggaa agcgttgcgc ggggtccggc gctcatcaac aatcggggag
     8341 tcagcaaggg gctgaaacgg gaaagcccct cccgaggaag gggccttata gaaggaaagg
     8401 gttatgatga agttcgtcat catactggtt gtgttgttac tgttaagttt cccgacttac
     8461 taacaactga tcagaggggg gagaaatcct cccttaccct tgttccttta ctttaggttg
     8521 aaaaaacaac agcgtcaata ggcctgcctg ctgcgaagcg agatctgtga accgctttcc
     8581 ggttagcctt ttttatcctg ttggatcttc ttgatgatgt tagtggtcga gcaaccgtct
     8641 tcgaagttga gcaccagcac ttcgccgccg ttggcccaga cttctttact cccggcaatt
     8701 tcttctggtt tatagtcgcc gcccttcacc agtaaatctg gcaggatccc ggcgatcaag
     8761 cgctgcggcg tgtcttcttc aaacgacacc acccagtcga ccgcttccag tgcgcccagc
     8821 acaatcatac gctgttcgag cgggttaacg gggcgggaat cccctttcag ccgtttggtg
     8881 gaggcatcgc tgttcacagc gacaatcaga cggtcaccca gtttgcgagc atttgccaga
     8941 taggagacgt gcccggcgtg caggatgtca aagacaccgt tggtcattac caccttttcg
     9001 ccgcgtttac gcgctgccgc tacagcaagc ttcagttcct cttcggtcat cacaccaaag
     9061 ccggtatctg cgcgcccacg cactgcattt tccagctcga tcggcgaaac cgtggaggtt
     9121 cccagtttgc cgaccaccac gccagccgcc gcattggcaa agaagcaggc ttcttccagc
     9181 gaattacccg ctgccagcgt tgccgccagg acgccaatca ccgtgtcgcc cgcaccggta
     9241 acgtcataca cttcctgcgc ctgggttggc atatgcagcg gcgctttacc cggttgcagc
     9301 agcgacatac cctgttcgga acgggtcact aacagagccg agagttcgta atcggcaatc
     9361 agtttcatgc cgcgctcaac aatctcttct tcggtcttac atttaccgac aacagcttca
     9421 aattccgaga gattcggcgt taacagcgta gcgccgcggt agcgctcaaa atcggtacct
     9481 tttggatcaa tcagcaccgg aacgcccgct ttacgcgcca gttggatcat ctgctgtacg
     9541 cttgccagcg cacctttggc gtagtcagaa agcaccagcg cgccaatcga actcagcgcc
     9601 tgattaatcc gctcgtgcag cggctgcgga tcaacacctt cgaaaccttc ttcaaaatcc
     9661 aggcggatca gctgttggtt gcgggaaagt acccgtaatt tggtgatagt cggatgcgtc
     9721 ggtacagaaa cgaagtcgca tttgacgttg acgtcggcca gagatttact cagcgcacgc
     9781 gccgcatcgt caatgcccgt caacccgacc aggcgtgcat tagcaccgag agaagcgata
     9841 ttcatcgcca cgttagccgc gccgcccgga cgttcttcga tggtattgac tttaaccacg
     9901 ggcaccggcg cttccggcga gatacggctg gtgggaccgt accagtaacg atccagcatc
     9961 acatcaccaa ccaccatcac tcctgcacgt tcaaactctg gcagcgttac tttcattcct
    10021 gtctcctgag agattcaaaa tttgcgcgcg ataataccat acttcattct tccaccagcc
    10081 acttctgcca gcttgcccgc accagatcac gctctgcggt gaagcaatcc tccgacacat
    10141 ggcccggcaa ttcctgtaat gccagatgat gaagttcatc gcgcagcgtc gtgtaagcac
    10201 gggtcagcgc catcgcttcc tgctcttcca taatgtcgtt ttgcgccagc agctccagaa
    10261 tacgcacgtt gtctgaccag cgcgttaact tcggtttttc atgagcgtag cgcaacacca
    10321 gatattgggt aataaattcg atatcggtaa ttccgccttc atcggctttg atatcaaagc
    10381 gattacgatg tttattgccg agatgagcgc gcattttctc gcgcatttcc cgcacttccg
    10441 tttgcagagt tttaccttca cgcggcagcg tcataatctc gcgacgcact gcgtcaaagt
    10501 gcgcggtgag ctgcggatcg ccgtacacta cacgcgcacg caccagcgcc tgatgctccc
    10561 acgtccaggc ctcgtttttc tgataatcag caaatgcttc tgcggatgtc accagcattc
    10621 ccgccgcccc ggacggacgc agtcgtgcat ccacttcata caaaatgccg gaagaggttc
    10681 gcgtactgaa cagatgcata atgcgttgcg ccagacgcag ataaaactgt cgcccgtcga
    10741 tttcccgctc gccgtcagtc atcgcatcca ttgggcaatc atggaggaag atcaggtcaa
    10801 gatcggaact gtagcctaac tcccagccgc ccagcttgcc gtagccgacc accgcaaaac
    10861 cacggccttc gcgatcgttg aggtgatttg gcttaccgta gcgggccacc atttgaaccc
    10921 acgcctgctg aacgacggca tctatcatag cctctgccag ccaggttaag tgatcgctca
    10981 ctttcatcac cggtagcgta ccagcgatat ccgctgcggc gatgcgtaac agctgcgcct
    11041 gtttgaactg acgcagcgcc tcaagctgtt gctcttcgtc atcttccggc acgcgcagca
    11101 aatactggcg caactcatcg cggtaggcat cggtcgccgt cggctggtaa agagtgtttg
    11161 gatcgagcaa ttcatccagc aataatggat aacgcgccag ctggctggca atcatcggcg
    11221 atgcggcaca cagagaaatc aaatgtttaa gcgccgcacg gaattcactg agcaattcta
    11281 aataggtggt gcgggtaaca atccccacca gcaaggcggt aatgcgcgac agcgtaacgg
    11341 cagcgtcttc acgcgcacag acatcactta gcagatgtgg catcagatgg tcgagcacct
    11401 gacgtcctcg cggcccgata gtacgcttat ccagctcttt gcggaaatca gcaatcagcg
    11461 ttaggacctg tttgcgatca tcctcgctaa gatgcgccag cactggcgta gtgtcatctt
    11521 cctgcaacgc atcctgccac agctcacgcc actgttccga cagcgactct tcctgagttt
    11581 cactttcgtc gtcgccaatc aattcattaa acacccggcg cacattggtc atatgtgcgg
    11641 tcagcgcccc ggtcagttgc ggccagtcag caaagtccat cgcccacgcc agccgcgcac
    11701 ggttaagctc atcagaagga agcgtttggg tttgttcgtc gttaatgctt tgcagcaggt
    11761 tttccagacg ccgcaggaac agatatgcca ctcgcaattg ttcagcatcg ttttcagaaa
    11821 gcagatgcag cgcggcaatg gcgctgagcg ttggcagtaa agagcgcgac tgcagcgacg
    11881 gttcgcgccc accgcgaatg agctggaaca cctgaacgat aaattcaatt tcgcgaatgc
    11941 cgcctgcgcc gagtttgata ttgtcggtca aaccacgtcg acgcacttca cgggcaatca
    12001 tccctttcat gttgcgcagc gactgaatca cgctgaaatc gatgtaacga cggaaaacaa
    12061 acgggcgcag catcgcacgc aactcgttag catagacgcc ttcgctatcg cccataatcc
    12121 gcgccttgac catcgcgtaa cgctcccagt cgcgcccctg ctcctggtaa taatcctcca
    12181 acgcggcaaa gctcagcacc agcgggccac tttcgccaaa cggacgcagc cgcatatcca
    12241 cgcgatagac gaagccatcc tgcgttggtt gatccaacac tttaatcagc cgctgcccca
    12301 tgcgggtaaa aaactgcgcg ttatccagtt cccggcgtcc gccctgcgta caaccatgtt
    12361 ccggccaggc aaaaatcaga tcgatatctg aggagaaatt cagttcccca ccgcccagct
    12421 tacccatgcc taaaatcagc agcggttgcg cttcgccctg cgcattgcac ggcgttcccc
    12481 attcgcggca gcaagcgtca tacagccagt cacgcgcggc gacaatcagc gtctccgcca
    12541 gatggctgag ctgctgcaat atgctctctt cagtaaccaa agccagcgtt tgcgcccaag
    12601 cgatgcgcac cataatgcgc cgccggaata gccgcagctc gcgcattaac ccggcttcgt
    12661 cactcacatt gctgagcgcc tcctgcaacc atgccgcgta atgctgccat tcatcagcct
    12721 gcggcgattg gctttccagt tccgtcagcc actctggatg cgcaatcacg ctgtcctgca
    12781 caaaatcact gaaagtaagt actgacttcg cctgtgcgct aagtgatttc tcggctaaag
    12841 gctctggcag ccgctcaaca acggtctgcc agtactgctg taacggtgaa gagagcggct
    12901 tcataagcga ttttatcctt gcctgattaa cgttttccgc tgtgcaacca gaacggttcc
    12961 tgattgtttg cctcattacg gaaatgttca atttcaatgc gttgcccggt cgcaatagcg
    13021 tgacgcagcc cctgccagtt ctccagccag gcttgcgcga cgacaggatc atagtaaccc
    13081 gccagcaaca gtattgagtc aatatcacgc atcagacgtg gcaactggtc acggtagcga
    13141 tcgcccaacg gctggcagaa aacgcttttc agttcagcgg catggcggga aagatggata
    13201 tcggcaaagc gtttgaagga atcgctcatt ttactctgcg ctttggcatc taaaaacggc
    13261 tgccaggctt tgcttaccaa ccattcggtc aacgccagct ttgccattgc cgtttcggta
    13321 gagtagaccg ccgtcacggc agaaaccgca gaagcgatgg tcgcttcgca ttgagtcagc
    13381 aaatcacgta agtgagtgct cgctttacgc ggcacaatgc caccgaacag catcagtgca
    13441 tggcgaacaa ggccaatggc tgccagcacc tgctctttcg ccgcatcgtt gccgcgtacc
    13501 cacagttctt catgatactg ccattgcgct aacgccagct cgagcgccgc ttccagcccc
    13561 tgttccacat cggcttttgc cgcaacatgc aaaatggtgg tcggtttgat ttcacgcgcc
    13621 ggattgccct gcgccagatg atagccacgc gccgctttgc tcaggctgcc ctggcgtaat
    13681 ccggtttgcg ataccagttg gttcgccagt ttcagcaccg cgcgcgtgtc gccgctaagc
    13741 agttccagtt ccagctcaca gataggttca gcaaattccc ccgctttcac ctccccctga
    13801 tcgagagcga tttcaattcg gctgtcatcg accgccacca gccatttttc gcgataaaaa
    13861 tcggtgctga acagcggttg tacgcgggaa gcgagatcgg cgggcaattc gccgttcggc
    13921 cagacttccg tcggtaactg cgccaggtcg agcgtcggtt cgctcaacgc cacgttatat
    13981 tccgggcgct ggtgtaagcc gcctgtcact cttcctgcaa ctttcatggt catctcatag
    14041 cgaccgtttt cgccacgaat acgtaagccc atatcgtgcc cacgcagcca gttatccggc
    14101 gtttcgtagt aaatattcag taactgcacg gggtcatggt gctcgccgcc cagcgtattg
    14161 agatggtcac gcaacgcctc aacggcactg tgattaacaa taaactttaa ttcgatttcc
    14221 tgagccatgg cctttactta tcggttatgt cacataagtg acgatgaacg gcgaacttaa
    14281 tacgatcttt tttgtcagta gatagtattt tgcgccaaat tgccatgcaa cgggcaattt
    14341 gacgggcgta aaagtttgaa gcagtggcaa gacctgacac agaggattcc gattgatgtc
    14401 gaatgctttg cgtagcgaca ctgatgccac tactatcgtt ccactttcca tgacaataac
    14461 gacagcctga tgccaaaatt acgcctgatc ggattaactt tactcgcact tagcgccact
    14521 gccgtctcac acgctgaaga aacgcgctat gtttctgatg aactgaatac ctgggtccgt
    14581 agcggtccgg gagatcatta tcgcctcgtg ggcacggtta acgccggcga ggaagtgacc
    14641 ttattacaaa ctgacgccaa caccaattat gcccaggtga aagacagctc tggccgtacc
    14701 gcctggatcc cgttgaaaca acttagcact gagccaagcc tgcgctcccg tgtgccagat
    14761 ctggaaaatc aggtcaaaac cctgaccgat aaactcacca atatcgataa cacctggaat
    14821 cagcgcaccg cagaaatgca gcaaaaagtg gcgcagagcg acagcgtgat caacggatta
    14881 aaagaagaaa atcagaaatt gaaaaacgag ctgattgtcg cgcagaaaaa ggtcgatgcc
    14941 gccagcgtac agttggatga caaacagcgc accatcatca tgcaatggtt tatgtatggc
    15001 ggtggcgtgc tggggcttgg cttgctgctc ggtctggtac tgccacacct gatcccaacg
    15061 cgcaaacgca aagatcgctg gatgaactaa atcgccttct tcgttgcact gtcttattat
    15121 taggaattgt gtatttgacg aagggatgat ggcgtgaaga tttatctggt cggtggtgct
    15181 gttcgggatg cattgttagg gctaccggtc aaagacagag attgggtggt ggtcggcagt
    15241 acgccacagg agatgctcga cgcgggctac cagcaggtag gccgcgattt tcctgtgttt
    15301 ctgcatccgc aaacgcatga agagtatgcg ctggcacgta ccgaacggaa atccggttcc
    15361 ggttacaccg gttttacttg ctatgccgca ccggatgtca cgctggaaga tgatcttaag
    15421 cgtcgcgatc tgaccattaa tgcgctggcc caggacgata acggcgagat tatcgacccg
    15481 tacaacggtc tgggcgatct gcaaaatcgt ctgttgcgcc atgtttcccc cgcttttggc
    15541 gaagatccgt tacgcgttct gcgcgtggcg cgttttgctg cgcgttatgc ccacctcggt
    15601 tttcgtattg ccgatgaaac tctgacgctg atgcgcgaga tgacccatgc gggtgaactg
    15661 gaacacctga cgcctgaacg ggtatggaaa gagacggaaa gcgccctcac cacccgcaat
    15721 ccacaggtgt tcttccaggt actgcgcgat tgtggcgcac tgcgcgtttt attcccggaa
    15781 attgacgcac tgtttggcgt tccggcccct gccaggtggc atccggaaat cgacacgggt
    15841 attcatacct taatgacgct ctcaatggcg gcgatgctga gtccgcaggt cgatgtccgt
    15901 ttcgcaactt tatgccacga tctcggtaaa ggactgacgc cgccagaact ttggccgcgt
    15961 catcatggtc acggtccggc gggtgtgaaa ttagtggaac aactctgcca gcgtctgcgc
    16021 gtgccaaatg aaattcgcga tttagccaga ctggtggctg agtttcacga tctcatccac
    16081 accttcccaa tgctgaaccc aaaaactatc gtcaaattgt ttgattccat cgatgcctgg
    16141 cgtaaaccgc agcgtgtcga gcaactggcg ctgaccagcg aggctgacgt gcgcggcaga
    16201 accggttttg aatcagcgga ctacccgcaa ggccgttggt tgcgcgaagc ctgggaagtg
    16261 gcgcagtcag tgccgacaaa agcggtcgtt gaagcgggat ttaaaggtgt ggagattcgc
    16321 gaggagctga cccgacggcg gattgcggcg gtagccagtt ggaaggaaca acgttgccca
    16381 aagcctgact gagtataaaa ctaaacgtta acacgaacca tcccctcgcc ccttcgggga
    16441 gagggttagg gtgaggggaa cagaccagca ccggtgcgaa caatcacccc caccccggcc
    16501 ctctccctac agagcgaggg agaaaaccag gcctgctccc gaaccttttc tcaatcaaaa
    16561 gaacacgacg tacaccgcag ccgccacaat aaagcgataa atggcgaacg ggataaacga
    16621 aatgcgctta atcaattgca ggaaggtttt aatcgctatc agcgccacca caaaagcggt
    16681 gataaaccca acggcaaaca tcgggatatc gccggttgtc aggaagcccc agcttttgta
    16741 gagatcgagc gccgttgcgc ccatcatcat cggtaccgcc agcaggaacg agaactcgga
    16801 agcagcgtaa cggctcacgc ccatcagcat cccacctgaa atggtggccc cggaacggga
    16861 gaaacccggc cacagcgcca gacactggaa acagccaatc ataaacgcct gacgataggt
    16921 catatcatca agacccggcg cacgcggctc tttcggcttc aggcattcgg cggcaatcag
    16981 caacaaaccg ccaacgacca gtgcatacat cacatttatc gggttaaaca atgacttaat
    17041 cgtgtcgtgg aacaacagcc ccagtaccac cgccggaatc atccccagca aaatgtggat
    17101 cagcgttaaa cgacctttgc tttctccttc gtgctgcaac gggcggccaa agtggatgcc
    17161 aatcaggcca aacagacgcc gccataacat cactactacc gccagaattg atcctaactg
    17221 gatcacaact tcaaaggttt tcgccgtgtc gccctcgaac cccaacaagt gaccgacaat
    17281 aatcatatgg cccgtgctgg ataccggcag aaattctgtc aatccttcga ccacacccaa
    17341 tattgccgct atcagcagcg agtgcatatc gctcatcaat aaacccctaa attattaaaa
    17401 tgtaccgctt gtccgaacta ctgcgtatga ccaggttata accgtttggt ttaacagctg
    17461 taaaattaat tattttcttt cagattattg ccacgctcaa tgattacgcc aacattcgcc
    17521 gcccgcgcca ctgcgcctgg cttgctgagt ttgatacgca cccacggcga gttgaagcgt
    17581 gctaacagca gctccgccac ctcttcagcc acgcgttcca ccagcgcaaa acgcgccccc
    17641 tcgacgtggc tgaccaccgt ttctgcaatg tcagcgtaac tgaggcaatc cgccacatca
    17701 tcacttttcg ccgctttacg gttatcccat gccatttcga tatcgaacac taacttctgt
    17761 tcgatggtct gttcccagtc gtaaacacca atagtggtga ttaccgaaag ttgctctata
    17821 aatacaatat ccatcacgtc ctgcctgctt tttggctaac cggataccac ttccggcgaa
    17881 atgtgcgtat tatccacaga ttcatcgttg aacacgaatt ttcaaaacgg aacagcttat
    17941 gagtgcaatc gcgcctggaa tgatcctcat cgcgtacctc tgcggctcca tttccagtgc
    18001 cattctggtt tgccgcttgt gtgggctgcc cgatccgcga accagcggct ccggcaatcc
    18061 aggcgcaacc aatgtgttac gtatcggtgg caagggagca gccgtagcag tactgatttt
    18121 cgacgttctg aaaggaatgt tgcccgtctg gggcgcgtat gaattaggtg tcagcccctt
    18181 ctggctaggc ttaattgcca tcgccgcctg tcttggacac atctggcccg ttttcttcgg
    18241 atttaaagga ggaaaaggcg ttgctaccgc ttttggtgcc atcgcaccca ttggctggga
    18301 tctcaccgga gtaatggcgg gaacctggtt actgaccgtg ctattgagcg gatactcgtc
    18361 gctgggagcg attgtcagtg cactgattgc tccgttttat gtatggtggt ttaagccaca
    18421 attcaccttc ccggtttcga tgctctcttg cctgatcctg ctgcgtcatc atgacaacat
    18481 ccagcgtctg tggcgtcgtc aggagacaaa aatctggacg aaattcaaaa gaaagcgcga
    18541 aaaggatccc gagtgatttc tggtggatct acatgacctg atagccttca tcgggcttgc
    18601 ccagccgttg ctggcaccat gccgccagaa attccacgca gacacgtaat ttcatgctgc
    18661 gatagagcgg ctcccggtaa acagcccaga tattggcgct ttgtgcatac tccggcaata
    18721 cttgcaccag tttgccactc tccagaaacg gcaacacatc ccactcggaa cgcagcataa
    18781 tccctttgcc ctccagcgcc cattgcaaca ctatctcgcc gctattggag gaaagatgcc
    18841 cgctaacctt caccgatttt ttctcctgac cgttccccaa ctcccatatt ccgtgggtca
    18901 tatcgcgctc tttggtcacc agacagtcat gacgacttaa ttcctgtaat gattgtggct
    18961 gcggatattt ttgcagatac tcaggtgctg cgcataatat tcttttattt ttcgttaaca
    19021 gatgcgcaat ataataatca ggaatttcgt cattaatacg aatatccaga tcaatattat
    19081 cctgcactaa atcaatttgc cgatcgaaca attcaaaatg cacctgtaac tcaggataat
    19141 tgcgcataag ttcggtaatg gctggcgcaa tatggctgcg cccaaaacca aaactacagc
    19201 caatacgaat catcccctcc gggcgcgttt tgatttgcgt gacgtcatcg accagtcgct
    19261 gatattgggt gaggatttcc agcgcatgtt cgtaacaacg ttggccactt tcagtcagcg
    19321 ccacgccgcg agcggagcgg ttcagcagcg ttgttgccag cgtattttca aggatttgga
    19381 tccgcttggt gacaaatgca ggcgtctgcc caagcgtcgc cgctgcggcg ctaaaactcc
    19441 cgctgtggac gatttctacc agtacctgca agtctttggc taaaggccag ctattcagca
    19501 tcgttaatta tccgcagttg tgataagcgc agtgtatttc gcaaaacatt gccattccct
    19561 gctctggcgc aatattcatt cttattttcg cgttgattca cgaactgtta attacatatt
    19621 cacggtggca aaaaatataa aaccacattt ttagtggtag tttgtggcgg tgaatttttc
    19681 cagacaaata caaaaactgg agttgccatg atgagcgaaa gtaataagca acaggcagtg
    19741 aataagttga cagagattgt cgctaacttt accgccatga tttctacccg aatgcctgat
    19801 gacgtggtgg ataaactaaa acagctaaag gatgccgaaa cgtcgtcgat ggggaaaatt
    19861 atctaccata cgatgttcga caacatgcaa aaagcgattg acctgaatcg tcctgcctgt
    19921 caggacaccg gggagattat gttcttcgtt aaagtcggtt cccgcttccc actgcttggc
    19981 gagctgcaaa gcatactcaa acaagccgtg gaagaggcaa ccgtcaaagc gccactacgt
    20041 cacaatgcgg tagaaatttt tgacgaagta aacaccggca aaaataccgg tagcggcgta
    20101 ccgtgggtca cctgggacat catccccgac aatgacgatg cggaaatcga agtttacatg
    20161 gcaggcggcg gctgcacgct acctggccgc tcgaaagtgt taatgccgtc agaaggctac
    20221 gaaggcgtag tgaaattcgt cttcgaaaat atctccaccc tcgccgtaaa cgcctgtcca
    20281 ccggtactgg tgggcgtggg catcgccacc tcggtggaaa ccgccgccgt gctctcgcga
    20341 aaagccattt tgcgcccgat tggcagccgc catcccaatc caaaagcggc agagctggag
    20401 ctacgcctgg aagaaggact caaccgtctg ggaattggcc cacaagggct gactggcaac
    20461 agttcagtga tgggcgtgca tatcgaatct gccgcccgcc atccgtcaac catcggcgtt
    20521 gctgtttcta caggctgctg ggcgcatcgt cgcggcacac tgctggttca tgccgatctc
    20581 acctttgaaa atctgtctca cacccggagc gcgttatgaa aaagatcctg acaaccccga
    20641 tcaaagctga agatctgcaa gatattcgcg tcggcgatgt gatctacctg accggtacgc
    20701 tggtgacctg ccgtgacgtt tgtcaccgcc gcttgatcga actgaaacgt ccgatccctt
    20761 acgatctcaa cggcaaagcg attttccacg ctggccccat cgtgcgcaaa aacggcgata
    20821 aatgggagat ggtttccgtt ggcccgacaa ccagtatgcg tatggaaagt tttgaacgtg
    20881 aatttatcga gcagaccggc gtgaaactgg tggttggcaa aggtggtatg gggccgctga
    20941 ccgaagaagg ctgccagaaa ttcaaggcgc tacatgtgat tttcccggca ggctgcgcgg
    21001 tgctggcggc aacccaggta gaagagattg aagaagtgca ctggacagaa ctcgggatgc
    21061 cggagtcact gtgggtctgc cgggtcaaag agttcggccc gctgattgtc tctattgata
    21121 cccacggcaa caacctgata gccgaaaaca aaaagctgtt cgccgaacgc cgcgatccca
    21181 tcgtggaaga gatctgcgag cacgtccatt acatcaaata accctcccgg agaggctcac
    21241 ccctctcctt tttcgcaggc ataacacgat gaaaccttcc actgaatggt ggcgatatct
    21301 tgcgccgctg gcggtcatcg ccattattgc tctacttccg gttcccgcag ggctggagaa
    21361 ccatacctgg ctctactttg ccgtttttac tggcgtgatc gttggactga tcctcgaacc
    21421 cgtgccgggt gccgtggtgg cgatggtggg tatctccatc atcgccatac tctctccctg
    21481 gctgctgttc agccccgaac agctcgccca gccaggcttt aaattcactg caaaatccct
    21541 ctcgtgggcg gtttccggtt tttctaattc ggttatctgg ctgattttcg ccgcctttat
    21601 gtttggcaca ggctatgaaa aaaccgggct tggacgccgc attgcgctgt ttctggtgaa
    21661 aaagatggga catcgcacgc tgtttctcgg ctatgcggtg atgttctccg agctgatcct
    21721 cgcacctgta acaccgtcca actcggcgcg tggtgcgggg attatctatc ccatcatccg
    21781 taacctgcca ccgctctatc aatcacaacc aaacgacagc agttcgcgca gcattggctc
    21841 gtacatcatg tggatgggga ttgttgccga ctgcgtgacc agcgccattt tcttgacggc
    21901 gatggcacca aacttgctgt taattggact gatgaaaagc gcatctcacg ccacgctgag
    21961 ttggggcgac tggttcctcg ggatgttgcc gctcagtatt ttgctggtgc tgctggttcc
    22021 ctggctggct tatgtgctgt acccgccggt actgaagtct ggcgatcagg tgccgcgctg
    22081 ggcagagacg gaactgcagg caatgggccc gctctgttcg cgtgaaaaac ggatgctggg
    22141 gctgatggta ggcgcgctgg tgctgtggat tttcggcggt gattatatcg atgccgcgat
    22201 ggtcggttac agcgtggtgg cactgatgct gcttctgcgc attatcagtt gggacgacat
    22261 tgtcagtaat aaagcggcgt ggaacgtttt cttctggctg gcctcgctta tcaccctcgc
    22321 taccggactc aacaacactg gttttattag ctggtttggc aaactgttag caggcagctt
    22381 aagcggttat tcgccaacga tggtgatggt agcgttgatt gtggtgtttt atctgctgcg
    22441 ctactttttc gccagcgcca cggcgtatac ctccgccctc gcaccgatga tgattgccgc
    22501 tgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc ctgatggttg gcgcggcaat
    22561 tggtctgggg agcattctta cactatacgc caccggaccc agtccgattt actacggtag
    22621 tggttatctg ccaacggtgg attactggcg actgggggcg atttttggac tgatattcct
    22681 cgtgttgctg gtgattaccg gcttgctgtg gatgcccgtg gtgttgcttt aagtgaggac
    22741 ggtgccggaa attgctctcc ggcaccggat agttttacgc agccggtaac tctgccagtg
    22801 gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc gcctgcttta aaccgcacca
    22861 ttccggcata ggcgatcatc gcgccgttat cagtacaaaa ttccggacgc gcgtagaaca
    22921 cttcgccgcg gcgttttttc atcatttccg ccagcttcgc ccgtaacgtg cggttagcac
    22981 tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg atccaacgct cgcttacact
    23041 taatcatcaa cgtatcgacc accgcatctt caaaggcgcg ggcgatatca gcacgcgtct
    23101 ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa ggttttcaga ccagaaaagc
    23161 tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa aacaaagcgc ccggcagtac
    23221 cctgagccgc cattttcgac agtaacggtc cgccaggata atccagcccc agcagcttcg
    23281 cggttttatc aaacgcttca ccggcggcat catcgataga ctcgccgagc agctcgtact
    23341 gaccaatgcc agtcacgctg attaactgcg tatgaccgcc ggacaccagc agcgcgacaa
    23401 acggaaattc cggcgggtta tcttccagca tcggcgctaa cagatgccct tccatatggt
    23461 gtaccgggat tgccggaacg ttccaggcaa acgccagaga acgccccacg gttgcgccaa
    23521 ccagcagtgc gccgactaat ccagggcctg cggtataggc cacagcatca atgtcttttg
    23581 ccgttaaacc agactccttt agcgccgcct ggatcaacgg tacggttttg cgcacgtgat
    23641 cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc gtgcaatttc acctgactat
    23701 acaattggtt ggctaacaaa cctttttcat cgtcgtaaat ggcgatgccg gtttcatcgc
    23761 aggaagtttc aatacccagt acacgcatga cttgttttac ctcgctttat taccgcgcag
    23821 tgtaggacca atgcgggttg atgtaaaact ttgttcgccc ctggagaaag cctcgtgtat
    23881 actcctcacc cttataaaag tccctttcaa aaaaggccgc ggtgctttac aaagcagcag
    23941 caattgcagt aaaattccgc accattttga aataagctgg cgttgatgcc agcggcaaac
    24001 cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa gtacgtgaaa acgagccgtt
    24061 cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa gcaggtgttc tggcggaagt
    24121 tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt aagcgcgcta aagcttctgc
    24181 agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca cgccgcactc gtctgtacta
    24241 attccccgag agcgttgctc tccgatcaga ccgagttgta gttgtaaggc cgtgcttccg
    24301 aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa aatcggggcc tatggctgga
    24361 cgaatcccac gcgtattcat taatgatctg ctggcacgca ctgacatcgt cgatctgatc
    24421 gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc acgcgtgttg tccattccac
    24481 aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac agttttacca ctgctttgga
    24541 tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact acgacaagct cgagttcgtt
    24601 gaaacggtcg aagagctggc agcaatgcac aatcttgaag tgccatttga agcaggcagc
    24661 ggccccagcc agatcgagcg ccatcagagg caaacgcttt atcagttgat ggacggtctg
    24721 aatacgtttt accaacaatc tttacaacaa cctgttgcca cgtctgcgcg ccagtatctg
    24781 gaaaaacgcg gattaagcca cgaggttatc gctcgctttg cgattggttt tgcgcccccc
    24841 ggctgggaca acgtcctgaa gcggtttggc ggcaatccag aaaatcgcca gtcattgatt
    24901 gatgcgggga tgttggtcac taacgatcag ggacgcagtt acgatcgttt ccgcgagcgg
    24961 gtgatgttcc ccattcgcga taaacgcggt cgggtgattg gttttggcgg gcgcgtgctg
    25021 ggcaacgata cccccaaata cctgaactcg ccggaaacag acattttcca taaaggccgc
    25081 cagctttacg gtctttatga agcgcagcag gataacgctg aacccaatcg tctgcttgtg
    25141 gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg gcattaatta cgccgttgcg
    25201 tcgttaggta cgtcaaccac cgccgatcac atacaactgt tgttccgcgc gaccaacaat
    25261 gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg ccgcatggcg agcgctggaa
    25321 acggcgctgc cttacatgac agacggccgt cagctacgct ttatgttttt gccggatggc
    25381 gaagaccctg acacgctggt acgaaaagaa ggtaaagaag cgtttgaagc gcggatggag
    25441 caggcgatgc cgctttccgc atttctgttt aacagcctga tgccgcaagt tgatctgagt
    25501 acccctgacg ggcgcgcacg tttgagtacg ctggcactgc cattgatatc gcaagtgccg
    25561 ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca acaaattagg catacttgat
    25621 gacagccagc ttgaacgatt aatgccaaaa gcggcagaga gcggcgtttc tcgccctgtt
    25681 ccgcagctaa aacgcacgac catgcgtata cttatagggt tgctggtgca aaaaccagaa
    25741 ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa ataagctccc tggacttggc
    25801 ttattcagag aactggtcaa cacttgtctc tcccagccag gtctgaccac cgggcaactt
    25861 ttagagcact atcgtggtac aaataatgct gccacccttg aaaaactgtc gatgtgggac
    25921 gatatagcag ataagaatat tgctgagcaa accttcaccg actcactcaa ccatatgttt
    25981 gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc gtgagcgcac gcatggttta
    26041 agcaacgaag aacgcctgga gctctggaca ttaaaccagg agctggcgaa aaagtgattt
    26101 aacggcttaa gtgccgaaga gcgatcggga agcccccgac agccgcactg agaggcagcg
    26161 gcaaatatat aagtacgccc tcgtaattat cgttggcggt aaacaaccgt tggatttcag
    26221 cgttaacggc tgaaggacat cgggtcaatc gcccaacacc aacctcatga aataagtgtg
    26281 gataccgtct tatggagcaa aacccgcagt cacagctgaa acttcttgtc acccgtggta
    26341 aggagcaagg ctatctgacc tatgccgagg tcaatgacca tctgccggaa gatatcgtcg
    26401 attcagatca gatcgaagac atcatccaaa tgatcaacga catgggcatt caggtgatgg
    26461 aagaagcacc ggatgccgat gatctgatgc tggctgaaaa caccgcggac gaagatgctg
    26521 ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga aatcgggcgc acgactgacc
    26581 cggtacgcat gtacatgcgt gaaatgggca ccgttgaact gttgacccgc gaaggcgaaa
    26641 ttgacatcgc taagcgtatt gaagacggga tcaaccaagt tcaatgctcc gttgctgaat
    26701 atccggaagc gatcacctat ctgctggaac agtacgatcg tgttgaagca gaagaagcgc
    26761 gtctgtccga tctgatcacc ggctttgttg acccgaacgc agaagaagat ctggcaccta
    26821 ccgccactca cgtcggttct gagctttccc aggaagatct ggacgatgac gaagatgaag
    26881 acgaagaaga tggcgatgac gacagcgccg atgatgacaa cagcatcgac ccggaactgg
    26941 ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt aacgcgtgac accatcaaag
    27001 cgaaaggtcg cagtaacgct gccgctcagg aagagatcct gaaactgtct gaagtattca
    27061 aacagttccg cctggtgccg aagcagtttg actacctggt caacagcatg cgcgtcatga
    27121 tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct ctgcgttgag cagtgcaaaa
    27181 tgccgaagaa aaacttcatt accctgttta ccggcaatga aaccagcgat acctggttca
    27241 acgcggcaat tgcgatgaac aagccgtggt cggaaaaact gcacgatgtc tctgaagaag
    27301 tgcatcgcgc cctgcagaaa ctgcagcaga ttgaagaaga aaccggcctg accatcgagc
    27361 aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc gaaagcccgc cgtgcgaaga
    27421 aagagatggt tgaagcgaac ttacgtctgg ttatttctat cgccaagaaa tacaccaacc
    27481 gtggcttgca gttccttgac ctgattcagg aaggcaacat cggtctgatg aaagcggttg
    27541 ataaattcga ataccgccgt ggttacaagt tctccaccta cgcaacctgg tggatccgtc
    27601 aggcgatcac ccgttctatc gcggatcagg cgcgcaccat ccgtattccg gtgcatatga
    27661 ttgagactat caacaaactc aaccgtattt ctcgccagat gctgcaagag atgggccgtg
    27721 aaccgacgcc ggaagaactg gctgaacgta tgctgatgcc ggaagacaag atccgcaaag
    27781 tgctgaagat cgccaaagag ccaatctcca tggaaacgcc gatcggtgat gatgaagatt
    27841 cgcatctggg ggatttcatc gaggatacca ccctcgagct gccgctggat tctgcgacca
    27901 ccgaaagcct gcgtgcggca acgcacgacg tgctggctgg cctgaccgcg cgtgaagcga
    27961 aagttctgcg tatgcgtttc ggtatcgata tgaacaccga ccacacgctg gaagaagtgg
    28021 gtaaacagtt cgacgttacc cgcgaacgta tccgtcagat cgaagcgaag gcgctgcgca
    28081 aactgcgtca cccgagccgt tctgaagtgt tgcgtagctt cctggacgat taatcggtag
    28141 gccggatcag gcgttacgcc gcacccggca ctagtccctc tgcacaaacg ccaccttttc
    28201 ggtggcgttt ttatcgccca cgcactacca gcgcctggtc cagctcgcga tatgcttcaa
    28261 ccagtttctc tagtgaaacg cgacttaaac cgctgggatt tggcagcacc caaatctgcg
    28321 tcgaaccaat ggtgagcgtt tgtttccccc actgtgcacc gcgctggctg aatccctgtt
    28381 catatgcttg tttgcccaga atcgccaacg cctgcggctg ataatcttca attttttcaa
    28441 tcagcttacg cccacctgcg tgcagctcct gctttgaaac ttcattggct tgcaccgttg
    28501 gacggtctac cagtttggtg acgccacaac gataatccag cagatgctgt gcctcctgcg
    28561 gcttcaactg acggtcggta aacccggcct gatatatcac cttccagaag cgatttgctg
    28621 gatgagcaaa gggaaaacca gtcccggcgg atgaaagccc agggttgata ccgcaaaaca
    28681 cgacccgtaa ccctggagcc aaaatatcct caaccatatt tactcctttc taacatcacc
    28741 aggaaaagta taacggattg aaaatacatt gtttataaaa acagcaggcg cacggtaatg
    28801 gctggattgc gacacggagt tactttataa tccgctacca tggcccctta gctcagtggt
    28861 tagagcaggc gactcataat cgcttggtcg ctggttcaag tccagcaggg gccaccaaat
    28921 cgacaccaag taaagacaag ttacggcaag ttaaacaaag caaacccgcg ccagacaagg
    28981 aacgcgggtt ttttattgtt cagtttgggc cagtttgata gcgtataagc aagattaaaa
    29041 aggcatacaa aaaggcatac gccggaaaaa acacagattt gcatatgcct ttaacagctt
    29101 atgggagttg aaccggtgac caaaaaagtt aagtctttat cacccgcaac gataaaaagc
    29161 ctccagcgaa aagccagcaa cgaaaaaaag gaccttttca tcactgacgg cgaagggctg
    29221 gaactggtga ttaagcacaa aagcgcctct tgcttgtggc gttaccgcta ctacaagcca
    29281 cacacaaaaa aacgggccat gttaagcatt ggcgcatatc cagaaatcag cattgcgggc
    29341 gcgcgaaaga taaaagacga atacagagcg cttctttctg gtggcattga cccaaaagag
    29401 cacagagaga aacagcgcct tagtgaagtg atgaagagag agaacacctt caggcatgta
    29461 gctacgctct ggtttgaact taaaaaaggg caaggactcg cagaagatac actacgcgat
    29521 atatggcgtt cgctggagcg taatgtattc ccgtcagtcg gtaacaggcc aatagcagac
    29581 ctgatagcaa gcaatttcat cgaagcatta aaaccattag ccgcccaggg gaaattagat
    29641 accgtcagaa ggacagcaca gcgaatcaat gaggtcatgt attacgccgt taataccgga
    29701 atgattgccg cgaatccggc atcagagata agcaaggcat ttaacgcgcc aagcgttaag
    29761 aacatgccag caatacgccc tgaagaactc cccgaattta tgcagaagct ggcaacggca
    29821 cgggtggaac gacaaacgcg gctggtcatt gagtggcagc tactgacaat ttgccgacca
    29881 ggtgaagcag ctggcgcgcg ctggtgtgaa attgattttg aggtgaagga atggcgcata
    29941 ccagagcaca gacttaaaca acgcagggaa cacgtaatac cactctcgaa gcaggcgtta
    30001 gccattctgg acgtcatgaa gccaataagc gggcatcgtg agtttatatt tccacacaac
    30061 agcgacccgc agcgccatat gtgcgtacaa acagcaaacg cagcaataaa acgcatgggt
    30121 tttagtgggc ggctggttgc tcatggcttg cgctcaatag caagtaccgc acttaacgag
    30181 caaggttttg actctgacgt aatcgaggcg gcattgtcac acatagataa aaacgaggtt
    30241 cgccgtgcgt acaaccgctc aacctatctc gagcagcgac gcccgttaat ggcgtggtgg
    30301 ggggattttg tagaggcagc tgccagcggg agcgtaagcg ccacgggtgg ctatcgtggg
    30361 ttaaaaatgg ttaacggtta agggcatggc aaacaaaaaa ataaaccttc ctgaaaggga
    30421 ttattacccg ttagacaagg cagccagaag gcttgagtgt gacgttgatg atttgcttca
    30481 tttcatcgct acgaggagga tgcgtttttt gtatcgcatt aatataaatg aaaaactgac
    30541 attaaagtat tatgaagggg taaagcctga gcttatcagt gctaataaaa cattatacca
    30601 gcattacaca aatgaatatt gcaatgtaat cattgatgcc acatctataa ataagctact
    30661 cactccaata gatgtttttt attatgcaca tgcaggactc aacataaact atcattactc
    30721 atcatatggt gattatttca gcatgaatat ttttgctgaa gatgagccag agataaaaaa
    30781 cgcacaaata ataacacccg caaaagaaat atatgttaaa gctgatggat tacttaatgt
    30841 aaaggctaac agcgcagaca ttgaaagatt atcatttcac gatacaacaa atactaaata
    30901 cattcagata atcgataacg atgacgagga tgattttaaa gctgcgattt tattacaggg
    30961 atgggatata tcacgtaacg atatttttat ttccagaagt gagatagatt tagccataaa
    31021 cggagggcgt accgcttcta ttgaaaacat ggatgaatta aacatcaata aaccaccccc
    31081 atcaaagaaa acaaccaatc ttcaggccag agttatcaaa agactaatca ccgctatagc
    31141 cggagaagaa gaggcgtcaa acccacgaag ggcgcttgaa aacggtcaca gcaagttaag
    31201 ccgcgcacta accaaagcag gcgttgatat cggtgccaca ccaacatgca taggtaagtg
    31261 gcttgcagac gttgacgact gacagacttc tgaacatatg accgcccgta atattacggg
    31321 cttttttcgt aatattccgc aatgccagct tttaccgtgt ttgcatatcc ttgaacaagt
    31381 ttaggcaagt tagcgcctga aacaaaagga acgcaaagta tgaacattac agaaaagaaa
    31441 atccttacaa aacgagaagt cgcaaatctt cttcgcctta gtacaacgag cattgacaca
    31501 atggttaagg ctggtgaatt tattcctggt tttaagcgtg gattacgccg cgttggctgg
    31561 tttgaggatg aggtgaaatc atggcttgag aagaaagtag aggagcgtta tcagcaggaa
    31621 taataaattc ccatatgtga aatataaaag ccaccagcgc agaaaataaa acgcctcacg
    31681 ttatgcaaaa cgggttaacc gtcgtgatgc cttgccgcat tcaattaacg gcaggtaaaa
    31741 aatacccgca atgcaataac actacgggcc atcaacggaa tataaggagt taaccgatgt
    31801 gtattgtata cagtcgtgtg tatattgcca caggtaacag gcatccgcgc caaatacccg
    31861 ccagagaaaa tcagttgatc gccagtatca aaaatggcta tgattcccgc gcacctcata
    31921 aaacgggtgt cgggattgga accccgctgg agataaaggc gatacagacg ccgaacgcgt
    31981 ctttttttgt atcggcgcac acgcacacct tatcaatggt gggctgtatg gggctgactt
    32041 cggtcaggct ggtttccttt atcgccagta gttccaactc cgtgcagtcc accgccagcg
    32101 agattggaac ctccggcggt gggtatatcc caacgataaa ggaggctgcc atcatggcta
    32161 ctacccctgc cctcgtacat tctcaaaccg cctttatctg gcgctttatc acctttggca
    32221 cgtcagaacg tcaaatcatc cacgtaaccg cctggacgga acgcgaagcg cgtaaccgtt
    32281 gcccgtccgg ttgtgtcgct gtattcgccg cccgtattcg tcagggggtg agtcatgctt
    32341 aaaaacatgt tgatcgcctc ttctttcggg ctgtatgatg ccggagtcgt ccacatggcg
    32401 accgggttta gcagcctgaa taccaaagcg gacagccgct tacattccga tatgcggttt
    32461 ttttgtgtcc gtaagcctac ccatacccgc attatggcgg ggcgtaacgg gggagccttt
    32521 gcgctcgctg gtttctttgg ttccagtctg ctaaccctgt tacgtctcgc caccccgttt
    32581 agcagcgtag tagcgagact ccttataaaa accaaaggag cctttcacat ggctgtattc
    32641 gcacgccctt actttgtctg gcgttttatg cagtgcctga caggcagcat tgcaatattc
    32701 accgttaccg ctgccactga gcacgaagca cgcgcacaac ttccgcacac ccatctgatt
    32761 tttgtcaccc ttattcgtga gggggtgagt tatgcgtgat gtgtgtaaca ccagaatact
    32821 tgaacaactg gttacaacca taaaagcagc tgacgcacgg gaagcgagag cgatgtttga
    32881 tgatgctgat ttctacgccc gcaaattgct tgaagggctg gctatcacag ggcgcttact
    32941 ttctggcatg agtgacagca ttgatcctga catggacgaa ctgcgcagcc tgggcgattc
    33001 catcgctgtt acggctgaac ttgtggccgg attcagtgag gttgtggagg tgtacaactg
    33061 gcgttgtcgt aacggagata tcagtgctga ttatcgccat tcgtgataag gcaacgacag
    33121 gcaccgctaa ccgcatttat gcagcaacgg cgtaactgac cagagcaaag gataaaaaaa
    33181 tgaacatgaa aaaaactggc ttaactgcca gcggcaagcc tcgccctgaa tttaagcgcg
    33241 gtgatattta ccgcgaccag tacggcggca cggtaatgat taagggcgtg gcggggcggt
    33301 gcgtcaccta ccgccgtaaa ggctacgaat atgactgcgt gatgcctgtt tatcagtttt
    33361 cccgtgattt ttctctggtg caggcggtac cgcgtaacgt gcccaccagc aacgtaaagg
    33421 cactggcaaa aatacagaag atgaaaaaga tgattaacgc attcaggggc aagaaatgaa
    33481 aagcgcaccg aacttaaaaa aacagcctta cgacaaaatg accgaggtca ttatttttgc
    33541 gggtagtgat gcctgggcac acgcgaagca gtggcaggag caggagggcc gacttgctgg
    33601 cgataatgtg ccacctgtat ggcttgggga gcagcaactg gcagaactgg cagacacgaa
    33661 aatcattgac gaggggcgct attgcgtccg gctgtacaaa gcaggccata tcagaccatc
    33721 aaatattaat gccatcgcgc aaaaactggc agcggcaggc gtacaggatg cgaattatta
    33781 ccccgatggg atgcacagcc agaagcggga gaactggcgc gaatatctgg aacgggagcg
    33841 cggggaaaag caggaagaaa aaggccacca gcgcaaaaca aagctaccga tgagcgttgg
    33901 ttctgccgga tacgatacac aacttgatta cgtggtaaag gggattattc cggcagtatc
    33961 gctgtgcagc atatacgggg cgagcggttc ctataaatcg ttccttgcgg gttcgtgggc
    34021 gtgtcacgtt tccacgggcc gccagtgggg aggccgcagg gtggcgcatg gcgctgttct
    34081 ctatgtggtt ggtgaaggcg gtatcggcgt tccgcgtcgt gtaaaagcct gggaggttgt
    34141 gcacggtgag caggtgaaaa atctgtatct ggtaaaccgc cccatctttc cggctgcccc
    34201 gcttgatgtt gatgaaatgg ttatcgctgc ccgccaggtg gaacgggaaa cgggtaaacc
    34261 tgtacgcatg attattctgg atacgctggc gcgttgcttt ggtgggaatg atgaaaatga
    34321 ttcccgtgat atgggggcat ttatccgtgg gtgtgacgag cttaaacggc gcacaggggc
    34381 cacggtgctg gtggttcacc attccggcaa ggatgagacg aaaggcgcgc gcggttccag
    34441 tgcatttcgt gcttcgctgg atgctgaata ccggatacgc agggaggacg caggaagcga
    34501 agcgctggtt atctcatgta ccaaaatgaa ggacgcggag gaactcaaag aagccgcata
    34561 tgacttacgc gtggtggagc tttttaccga cgctgacggg gagttaatta cgtcgctggt
    34621 ggtggtggat aagccgcggc atcccgttga acctgagcgc atcgaggagg cagggaacaa
    34681 gacggaaaac cataccgcgc tatgggggtg catacgttca cgcacacaga acggcgacaa
    34741 gtgcacgatc ccgctgttac gtgatgacat gaaacggctt gggtatgacg tgaaaaacat
    34801 gcggcgatgg ttagcaaaac tggagaaaga cgacgttatc tatatcgacg gtgatgatgt
    34861 aggcccactg taaaaaagtg agtagaaaag tgagtaatgg tgagattttt acaaaatatt
    34921 aacacgatta ctcactttct cacctgtata taggcaaaaa agtgagtagt aaaaaatcac
    34981 atataaaaca cgcacataga aaacccgaaa atcacaggtg agacgaagtg agcgattcca
    35041 aaaagtgagt aaaaaaagtg agtagtggtg agtaaacagc aaaaaacaca gagcagagca
    35101 gcacgaaaac acagaaaatc actttatttt tatttaagtg ttttgctcat gaacgaaggg
    35161 ccacaacacg gcagaaagac acgcgaaagc cccctggctg ataagtgcat tcatgagcac
    35221 agcagcaaca aactttacca cgctgacaga gcgggagatt gcagaagcgg aacaggtaac
    35281 aaccatcaac gttaaaaccg gagaggtgga atcatgacag cgcagatagc agcttacggg
    35341 cgactgattg acgacccgca gacaaaacag accagtaagg gcacaccgat gacgctggca
    35401 cgtatggcgg tatcgctgcc acgcagtcag gcacaggacg gacaggcgac gttatggcta
    35461 tcagtcctgg catttggtaa gcaggccgac caactggcag gacatgagaa aggcaacgta
    35521 atcagcgtat ccggtgtgat gcaggtcagt cagtggaccg gacagaacgg ggaaacgcgg
    35581 cagggttatc aggtcattgc agacagcgta atcagtgcga aaacggcgcg accaggtggc
    35641 aacagacgca ggaccacagg cacacagggt aatcagccac cagcgggagg cgatgaccct
    35701 tacggtgaca atattccgtt ctgaggggtg aacgatggta catgacagca tagcggaaga
    35761 actggaggcg aagggctttt accggagagc ggcggcgcga tgggctgaag tgatgatgct
    35821 ggtggagact gacagggaac gcgaacgggt ggcaatgcgc agggcagagt gttacgggaa
    35881 gacgcagagg ccaccggagc agccggatga tttaggcggc ctgaaacggg cgattaaacg
    35941 cactgaaaaa gaaatgggga tagacgggag aggcaaaaaa atctggcgta gctaccagaa
    36001 gtaagcacag agccggaaca gtcggttagc ctttctgatt gtgtgcgtgt atatcgtttt
    36061 attgcgacgt taaattaaac tggtaggacc agatacacca acgcaagagg ctaccggatg
    36121 aacgagcaaa cccatcacgt accaaaagac atcgagcggc ttaaattcat cgacgccata
    36181 gacaacggag ataaaccagg tgatgcatgg tgtgattcgc ttgctgatat cgtgtcgcat
    36241 tcagtagcgg agcttacccg taatggcatg gaggaggcag acgctttcag aatcgcaaag
    36301 cgggttgtcg ctgcaatcgc cttctatgct ggtgggcgaa gtctttacct tccaacaggc
    36361 agatcacttt tcgcagcatt gcgcgaatac gagatttact acaggtggga taacggcgaa
    36421 aaattgcagg cattacgcaa agagtacgca atatccgaaa cccatattta cgacatcata
    36481 cgacgactga gaaagaagca cgccaataaa atcacgcaag actaaccaca gataccagca
    36541 gcaaccgtta tatttgcgcc gctatcgcaa aaaatcccct ttcagggcat cacggacgcc
    36601 acaaaacacc aaaacgcatc agagagcgaa tcaggcgctt taatgatgat ttgttttttt
    36661 gtgaaagaag gtgaaagaag gtgatttttg tgaaattaag cgaaatcacg tgaaatcagg
    36721 tgaaataatg atacccgttc atctggtgct accagacgaa acagacttta cccgcgcata
    36781 atgcatgatt actattttgc ctggctgtat ggtgtactct taatcggtaa agcggcataa
    36841 accctaacgg gccatatgtt tatgtgggtt cgacccccgc caccaacagc caactacctt
    36901 atcgcttttt tgctggtgta ctcttaatcg gtaaagcggc acccgcttgt atagcttcca
    36961 gaaagcggga tgtttatgtg ggttcgaccc ccaccaccag cagccaacaa cctcatcgct
    37021 tttttgctgg tgtacgctca acgggtagag tcggtgccag cctgtacagg ctgatcatgt
    37081 atgcgggttc gacccccgcc accagcacac caaaacgagc gccacagagc gccacatcaa
    37141 ccagcattaa aaccgcccta aaaccatttt caaacgttcg acagcgcaca catgaacgcc
    37201 atcggtttgc atgttcacat tgatacccgt tatcgacgat caaacaacaa gggtatcttt
    37261 ttcaaaaaag gcatacaaaa aggcatacaa aacgaaaatc aaaaacacat aatctttatt
    37321 tatcaataaa atatgcattc agttcaaaga cagcagggcc accagatata gcaaaggctg
    37381 acgagaaatc gtcagccttt ttctttttat atatcagtta ctttgcgtgc cagtaagccg
    37441 ctgcacgtac ccgctgtggg tcatactgtt ccgcttcaaa gcggcggctt aaattcttaa
    37501 cgactttacc ttcgccggtt atccagatga agtaatcatc ggcagggatt tgcatctgcg
    37561 ccagacgcgc atctaccgcc tgctcatcat gtgccagcca ttcgatatta aaaccatcaa
    37621 ggtgcgcgag ataatcctga caggcgttat cccgcacgct aaccagcgca ctaacttgcg
    37681 gtttaacggc aagtttgctc aacgtttcca ggcggcggcg cagtgcgggc attccggatt
    37741 catcgcagac atacagctga tacgcgtaat cttccggcac caccagcgaa ccgcgcggac
    37801 ctgccaccgt aagtttatcg cccggttgcg cctgcatcgc ccagccgctg gcgaccccac
    37861 cgtcgtgaat aaagaaatcg atcgccagtt catggcgtag ttcgtcatac agtggcgtat
    37921 agtcacgcga cggtgggcgt ggtccttccg gccagacgat gccctcttcc gttaccgttg
    37981 gcggcacaaa gtgagcgtca ggttgaggaa agaagagttt gctgtgatcg tcaaagccgc
    38041 gcgatgtaaa accgtccagc gcctcgccgc cgaggacaat gcgctgaaaa ccggcgctga
    38101 tgcgctcaac gcgtaacaca gtcagttcac ggaagcgcag atcattgcga acgcgctgcg
    38161 ggtagcgggg ggagttattc attgttatcg ccttcgtgat agtaatcaga tatatctaaa
    38221 taattttcgt aaatgataat gattgttaat catgataaat gcaagcgatt tgtagaactg
    38281 atatatctga agtatgtgaa ggcattcagt gccatagcaa tgctgccgga tgcggcgtga
    38341 acgtcttatc cggcctggat gtgaggtgct tccacgttta aacatcattt ttgtacttgc
    38401 agtaatgctc acatcagata taaattagat atatctaatc aagcaaaagg aggctgatat
    38461 gagccatcat cacgaagggt gttgtaaaca tgaaggccag ccacgccatg agggctgctg
    38521 caaaggtgag aagtcagaac acgagcactg cggacacggt caccagcatg aacacggtca
    38581 atgctgcggt ggtcgccacg gtcgcggcgg cggtcgtcgg caacgtttct ttggtcacgg
    38641 tgaattgcgt ctggtgattc tggatattct ctcgcgcgat gacagccacg gttacgaatt
    38701 gattaaagcg attgagaacc taacccaggg gaattacacc ccaagcccgg gcgtcatcta
    38761 cccaacgctg gattttctgc aggagcagtc gctgattacc atccgcgaag aggaaggtgg
    38821 taagaagcag attgcgctga ccgaacaagg cgcgcagtgg ctggaagaaa accgcgaaca
    38881 ggtggagatg attgaagaac gcatcaaagc gcgttgcgtt ggcgcggcgc tgcgccagaa
    38941 cccgcaaatg aagcgggcgc tggataattt taaagcggtg ctggatttac acgtcaacca
    39001 gagcgatatc agtgatgcac aaataaaaaa gatcattgcg gtgatcgacc gcgccgcttt
    39061 tgatattacg caactggatt aatcgccgca tccgccagtg gcgcggtgca attgccggat
    39121 gcgacgcttg acgcgcctta tccggcctac acccgctaca caccccgcag gcctgataag
    39181 atgcgccagc atcgcatcag gcattgtgct ccaaccgccg gatccggcat accgattaat
    39241 gcagcaccgt caccgcgtct tccagtcggc tggcgcggtg tttcaccatc tccgacacct
    39301 gcgcactctc ttccaccagc tcggcatttt tctgggtgat caggttaagc tcatccactg
    39361 cacgggtcag gctggaaagc ccatcggcct gttccagcgt tgaatggcta atctgggcga
    39421 tcaactgggt gacgtttttc acctgtgcca caatatcttc catcgtccgt ccggcggcgt
    39481 gtacctgctg cgaaccggat tgcaccttat cagcactggc atcaatcagc ttgcgaatat
    39541 cgttggcagc attagcgctg cggctggcta aatgacgcac ttcccctgcc accactgcaa
    39601 aacctttgcc ctgttcgccg gcacgcgccg cttccaccgc cgcattcagg gccagaatat
    39661 tggtctgaaa cgcaatatcg ttaatcagcg aagtaatggt gccaatgcgc tgggtactgt
    39721 cggcgatatc gtccattgtc ttgatcaccg tggtcatcgc ctccccaccc tgcactgccg
    39781 cattactggc ggtgatagaa agtttatcgg cagccgacgc cgccatttgg ttcatggtcg
    39841 ccaccgtttg ctgaacgtta tcaactgtct gctgggtatg ttcgttcagt tcatcggtgc
    39901 ctttcgccag cgtctcactg ccatttctga cactggacac ctggcttgag acatcgttaa
    39961 ttagccaacg gcacatcagg ccaagctgcc ctaccgcgcg taatgtcagc cccagctcat
    40021 cgctgcgatt caggtgctca acactattac gctctccggt cgccaccttc agtgcctgac
    40081 gggcgacatt ttctatcggg cgcacaatct gccattcaaa acaagcgctt gccaacaata
    40141 ccactaacac acagaggaaa tacgtcacca ccggggcagc aacaaaccaa agcatggccg
    40201 ccagcaagat aaacatcagg gtcatcactc cacgcgcccg ccagcgaagc ggtaatgaag
    40261 gcagtttacc cagccagcct ttacgcacca ccaggccttt atgaatacgc ttactggtac
    40321 gtccggcgtt cagcgctttg tacagcggct ccaccgccgc aatctcttca tccgtcgccc
    40381 gggtacgaat cgacatatag ccactgattt ttccctcgcg caccatcggt accgcattgg
    40441 cccgcaccca ataatggtca ccatttttgc ggcgattttt cacgatgccg ctccagggct
    40501 cccctttttt cagggtgaac cacatatccg caaacgccgc ttttggcata tccgggtgac
    40561 gcaccatgtt gtgcggctgc ccttgtaact cttgcaaggt aaagccgctc acctgcacaa
    40621 aagtgtcatt agcatgagtg atatagcttt gcagatcggt agtggacatc agagtggtat
    40681 cgtccgccag cggggtattt tgctgggtga catacggatg agaagacatg ttcgcgtcct
    40741 gtgcaggtta tatggttgtt aacttcttgt cagagtttat gtcggccccg ctgcggttat
    40801 ctttaaccga ttaatttgat ttagatcgca atttgcgatt aaagcacaaa tctaattctt
    40861 tgatttaaaa tactttcact ctgttactat acgaaaacgt taattatctt gcccaaaaat
    40921 caggcaatta ttgccctgaa aacgtgcatt tgcgcagcaa tcatcaaatc catacccgac
    40981 aaaaaccgtg caaaataaca acaaatgtta acagatagca ttaaatattg cacaaatgat
    41041 aaccgaattt gtgtttatcc cgattttcgc gatcgcagcc ggagtggcgc aatccctgca
    41101 atacttaaat cggtatcatg tgatacgcga gcctccggag catattttga acaggttacc
    41161 ttcgagcgca tcggctttag cgtgcagcgc ccacgccctg aatctcattg agaagcgaac
    41221 gctggatcat gaggagatga aagcacttaa ccgagaggtg attgaatact tcaaagagca
    41281 tgtcaatccg gggtttttag agtatcgcaa atctgttacc gccggcgggg attacggagc
    41341 cgtagagtgg caagcgggag gtttaaatac gcttgtcgac acccagggac aggagtttat
    41401 cgactgcctg ggaggttttg gaattttcaa cgtggggcac cgtaatccag ttgtggtttc
    41461 cgccgtacag aatcaacttg cgaaacaacc gctgcacagc caggagctgc tcgatccgtt
    41521 acgggcgatg ttggcgaaaa cccttgctgc gctaacgccc ggtaaactga aatacagctt
    41581 cttctgtaat agcggcaccg agtccgttga agcagcgctg aagctggcga aagcttacca
    41641 gtcaccgcgc ggcaagttta cttttattgc caccagcggc gcgttccacg gtaaatcact
    41701 tggcgcgctg tcggccacgg cgaaatcgac cttccgcaaa ccgtttatgc cactgctgcc
    41761 gggcttccgt catgtgccgt ttggcaatat cgaagccatg cgcacggctc ttaacgagtg
    41821 caaaaaaacc ggtgatgatg tggctgcggt gatcctcgaa ccgattcagg gtgaaggtgg
    41881 cgtaattctg ccgccgccgg gctatctcac cgccgtacgt aagctatgcg atgagttcgg
    41941 cgcactgatg atcctcgatg aagtacaaac gggcatgggg cgcacgggca agatgttcgc
    42001 ctgcgagcat gagaacgtac agccggatat cctctgcctt gccaaagcgc tcggcggcgg
    42061 cgtgatgccg attggcgcga ccatcgccac tgaagaggtg ttttcagttc tgttcgacaa
    42121 cccattcctg cataccacca cctttggcgg caacccgctg gcctgtgcgg cggcgctggc
    42181 gaccatcaat gtgttgctgg agcagaactt accggctcag gctgagcaaa aaggcgatat
    42241 gttgctggac ggtttccgtc aactggcgcg ggaatatccc gatctggtac aggaagcgcg
    42301 tggtaaaggg atgttgatgg caattgagtt tgttgataac gaaatcggct ataactttgc
    42361 cagcgagatg ttccgccagc gcgtactggt ggccggaacg ctcaataacg ccaaaacgat
    42421 ccgcattgaa ccgccactga cactgaccat tgaacagtgt gaactggtga tcaaagcggc
    42481 gcgtaaggcg ctggcggcca tgcgagtaag tgtcgaagaa gcgtaatatc aaatcggatg
    42541 gcgatacgac gtcgccatcc gatcttttta tacgacgcgt acgcccgcag gcatcatccg
    42601 ctccggcgtt aacaacacgc tttcactgcc gtcatcggtt tccgcacaca acagcatgca
    42661 ttccgacgtt tcaccacgca ttttcgcttt ttgcagattg cataagacca ccaccgtttt
    42721 ccccatcagc tcttcttcgc tgtagtacgg caccaggctg gtcacggttt gcagtgtttt
    42781 ttcccccaca tcaacctgta cgatgtacag cttgtcggcg ttttcatggc gtttcacttc
    42841 cacaatcttt ccgacgcgca tttccagacg tgcaaaatca gcgtaagcca cggtttccat
    42901 tgtttcctcc cttcgggtaa aattttacta aactatagca aaagtttttc tcaatcctgt
    42961 aggctaaaaa tggagaatgc aggcgtgatc acattcgtga gccgctgtgt taccgttaca
    43021 gcgtcaaaga aacgcgcttt atttactgaa aacaggtgac ccgataagca cttcctctac
    43081 aatgggggcg cacatcaggg aaagtaaaaa aggtaaacat ggcaacacta aaagacatcg
    43141 caatcgaagc tggcgtatcc ctggcgacag tatccagggt cttaaatgac gatccgacat
    43201 tgaatgtgaa agaagagacg aaacatcgca ttctcgagat cgccgaaaag ctggagtaca
    43261 agaccagtag tgcccgtaaa ctccagacag gtgcagtcaa ccaacaccat attctggcta
    43321 tctacagcta ccaacaggag ctggagatca acgatcctta ctatctggcg atccgccacg
    43381 gcattgaaac tcagtgcgaa aagctgggca ttgagctcac caactgttat gaacacagcg
    43441 gattaccaga cattaaaaac gtcaccggta ttttaattgt cggcaaaccc acgcccgccc
    43501 tgcgcgccgc cgcctgtgcg ttgaccgaca atatctgttt tatcgacttt cacgaacccg
    43561 gcagcggtta cgatgcggtg gatatcgatc tggcacgcat cagtaaagaa atcatcgact
    43621 tctatatcaa ccagggcgtt aatcgtattg gttttattgg cggtgaagat gagcctggca
    43681 aggcggatat tcgtgaggtc gcctttgcgg aatatggccg actgaaacaa gtggtacgcg
    43741 aagaggatat ctggcgcggc ggtttttcca gttcgtcggg ttatgaactg gcaaaacaaa
    43801 tgctggcgcg agaagactat ccgaaggcac tgtttgttgc ttccgattcc attgctatcg
    43861 gcgtactgcg ggcaattcat gaacgaggcc tgaacatccc acaggatatt tcgcttatca
    43921 gcgttaacga tatccccacc gcgcgattta cctttccgcc gctctccacc gtgcgcatcc
    43981 attccgagat gatgggaagt cagggcgtta acctggtgta tgaaaaagcc cgcgatggtc
    44041 gcgcgctgcc gctgttagtc ttcgttccca gcaaattaaa actgcgcggc acgacccgtt
    44101 aaatcccctt acacactgtc cggcaatcgt ttttgccgga cagtgctgcc gtttattttc
    44161 gtgatccagt taaagtaaat gcatttacct gctacttttt agtaaaaatt ttactaaact
    44221 ccccagcaat tacacaaact accatcacca tgaatggttc cgatttctct ctaccgggag
    44281 gccctatgaa tcgctgggaa aacattcagc tcacccacga aaaccgactt gcgccgcgtg
    44341 cgtacttttt ttcatatgat tctgttgcgc aagcgcgtac ctttgcccgc gaaaccagca
    44401 gcctgtttct gcccttaagc ggtcagtgga atttccactt ttttgaccat ccgctgcaag
    44461 taccagaagc cttcacctct gagttaatgg ctgactgggg gcatattacc gtccccgcca
    44521 tgtggcaaat ggaaggtcac ggcaaactgc aatataccga cgaaggtttt ccgttcccca
    44581 tcgatgtgcc gtttgtcccc agcgataacc caaccggtgc ctatcaacgt attttcaccc
    44641 tcagcgacgg ctggcagggt aaacagacgc tgattaaatt tgacggcgtc gaaacctatt
    44701 ttgaagtcta tgttaacggt cagtatgtgg gtttcagcaa gggcagtcgc ctgaccgcag
    44761 agtttgacat cagcgcgatg gttaaaaccg gcgacaacct gttgtgtgtg cgcgtgatgc
    44821 agtgggcgga ctctacctac gtggaagacc aggatatgtg gtggtcagcg gggatcttcc
    44881 gcgatgttta tctggtcgga aaacacctaa cgcatattaa cgatttcact gtgcgtaccg
    44941 actttgacga agcctattgc gatgccacgc tttcctgcga agtggtgctg gaaaatctcg
    45001 ccgcctcccc tgtcgtcacg acgctggaat ataccctgtt tgatggcgaa cgcgtggtgc
    45061 acagcagcgc cattgatcat ttggcaattg aaaaactgac cagcgccagc tttgctttta
    45121 ctgtcgaaca gccgcagcaa tggtcagcag aatcccctta tctttaccat ctggtcatga
    45181 cgctgaaaga cgccaacggc aacgttctgg aagtggtgcc acaacgcgtt ggcttccgtg
    45241 atatcaaagt gcgcgacggt ctgttctgga tcaataaccg ttatgtgatg ctgcacggcg
    45301 tcaaccgtca cgacaacgat catcgcaaag gccgcgccgt tggaatggat cgcgtcgaga
    45361 aagatctcca gttgatgaag cagcacaata tcaactccgt gcgtaccgct cactacccga
    45421 acgatccgcg tttttacgaa ctgtgtgata tctacggctt gtttgtgatg gcggaaaccg
    45481 acgtcgaatc gcacggcttt gctaatgtcg gcgatatcag ccgtattacc gacgatccgc
    45541 agtgggaaaa agtctacgtc gagcgcattg ttcgccatat tcacgcgcag aaaaaccatc
    45601 cgtcgatcat catctggtcg ctgggcaatg aatccggcta tggctgtaac atccgcgcga
    45661 tgtaccacgc agcgaaggcg ctggatgaca cgcgactggt gcattacgaa gaagatcgcg
    45721 atgctgaagt ggtcgatatt atttccacca tgtacacccg cgtgccgctg atgaatgagt
    45781 ttggtgaata cccgcatccg aagccgcgca tcatctgtga atatgctcat gcgatgggga
    45841 acggaccagg cgggctgacg gagtaccaga acgtcttcta taagcacgat tgcattcagg
    45901 gacattatgt ctgggaatgg tgcgaccacg ggatccaggc gcaggatgac aacggcaacg
    45961 tctggtataa attcggcggc gactacggcg actatcccaa caactataac ttctgtcttg
    46021 atggtttgat ctattccgat cagacgccgg gaccgggcct gaaagagtac aaacaggtta
    46081 tcgcgccggt aaaaatccac gcgctggatc tgactcacgg cgagctgaaa gtcgaaaata
    46141 aactgtggtt taccacgctt gatgactaca ccctgcacgc agaggtgcgc gtcgaaggtg
    46201 aaacgctcgc gacgcagcag attaaactgc gcgacgttgc gccgaacagc gaagccccct
    46261 tgcagatcac gctgccgcag ctggacgccc gcgaagcgtt cctcaacatt acggtgacca
    46321 aagattcccg cacccgctac agcgaagccg ggcattctat cgccacttat cagttcccgc
    46381 tgaaggaaaa caccgcgcaa ccagtgcctt tcgcaccaaa taatgcgcgt ccgctgacgc
    46441 tggaagacga tcgtttgagc tgcaccgttc gcggctacaa cttcgcgatc accttctcaa
    46501 aaatgagtgg caaaccgaca tcctggcagg tgaatggcga gtcgctgctg acccgcgagc
    46561 caaagatcaa cttcttcaag ccaatgatcg acaaccacaa gcaggagtac gaagggctgt
    46621 ggcaaccgaa tcatttgcag atcatgcagg aacatctgcg cgactttgcc gtagaacaga
    46681 gcgatggtga agtgttgatc atcagccgca cagttattgc cccgccggtg tttgacttcg
    46741 ggatgcgctg cacctacatc tggcgcatcg ctgccgatgg ccaggttaac gtggcgcttt
    46801 ccggcgagcg ttacggcgac tatccgcaca tcattccgtg catcggtttc accatgggga
    46861 ttaacggcga atacgatcag gtggcgtatt acggtcgtgg accgggcgaa aactacgccg
    46921 acagccagca ggctaacatc atcgatatct ggcgcagcac cgtcgatgcc atgttcgaga
    46981 actatccctt cccgcagaac aacggcaacc gtcagcatgt ccgctggacg gcactgacta
    47041 accgccacgg caacggtctg ctggtggttc cgcagcgccc aattaacttc agcgcctggc
    47101 gctataccca ggaaaacatc cacgctgccc agcactgtaa cgagctgcag cgcagtgatg
    47161 acatcaccct gaacctcgac caccagctgc ttggcctcgg ctccaactcc tggggcagcg
    47221 aggtgctgga ctcctggcgc gtctggttcc gtgacttcag ctacggcttt acgttgctgc
    47281 cggtttctgg cggagaagct accgcgcaaa gcctggcgtc gtatgagttc ggcgcagggt
    47341 tcttttccac gaatttgcac agcgagaata agcaatgagg atcatcgata acttagaaca
    47401 gttccgccag atttacgcct ctggcaagaa gtggcaacgc tgcgttgaag cgattgaaaa
    47461 tatcgacaac attcagcctg gcgtcgccca ctccatcggt gactcattga cttaccgcgt
    47521 ggagacagac tccgcgaccg atgcgctatt taccgggcat cgacgctatt ttgaagtgca
    47581 ttactacctg caagggcagc aaaaaattga atatgcgccg aaagagacat tacaggtagt
    47641 ggaatattat cgtgatgaaa ctgaccgtga atatttaaaa ggctgcggag aaaccgttga
    47701 ggtccacgaa gggcaaatcg ttatttgcga tatccatgaa gcgtatcggt ttatctgcaa
    47761 taacgcggtc aaaaaagtgg ttctcaaagt caccatcgaa gatggttatt tccataacaa
    47821 ataacaacta cggcggcaaa aggagtttgc cgccaccgct accctacaca ttttcggaga
    47881 tgtgttatgt ctgataccaa acgtaataca atcggcaaat tcggcttgct ctcgctgact
    47941 tttgccgccg tttacagctt taacaacgtt atcaataata atattgagct tggactggcc
    48001 tcggcaccga tgtttttcct cgcgacgatt ttttatttta ttcccttctg tctgatcatc
    48061 gcagaatttg tttcgttaaa taaaaactca gaagccggtg tctacgcgtg ggtaaaaagt
    48121 tcgctgggcg gacgttgggc atttattact gcctatacct actggttcgt aaacctgttc
    48181 tttttcacct cactgttgcc gcgcgttatt gcttatgctt cgtatgcctt cctcggatac
    48241 gaatatatta tgacgccggt tgccaccacc attatcagta tggtgctgtt cgccttctcc
    48301 acctgggttt ccaccaacgg ggcgaaaatg ttggggccaa ttacctccgt cacttcaacg
    48361 ctgatgctgc tgttaacgct ctcctacatt ttactggcag gtacggcgct ggttggcggc
    48421 gtacagcctg ctgaccccat caccgttgac gcgatgatcc cgaacttcaa ctgggcgttc
    48481 ctcggcgtta ccacctggat ctttatggcc gcaggtggcg cggagtccgt cgctgtgtac
    48541 gttaacgacg tcaaaggcgg ttcgaaatcg ttcgttaaag tgatcatcct cgccgggatt
    48601 tttatcggcg tactgtattc cgtctcctcg gtgctgatta acgtcttcgt cagcagcaaa
    48661 gagttgaaat ttaccggcgg atcggtgcag gtattccacg gcatggcggc gtattttggt
    48721 ctaccggaag cgttgatgaa tcgctttgtc ggtctggtgt cctttaccgc gatgttcggt
    48781 tccctgctga tgtggaccgc aacgccggtg aaaattttct tctccgaaat cccggaaggc
    48841 atctttggta agaaaaccgt cgaactgaac gaaaacggcg ttccggcgcg cgcagcgtgg
    48901 atccagttcc tgatcgtcat cccgctgatg attatcccga tgctcggttc caataccgtg
    48961 caggatctga tgaatactat tattaatatg accgccgcag cgtccatgct tccgccgtta
    49021 ttcatcatgc tggcttacct gaatttacgc gccaaattag atcacctgcc acgcgatttc
    49081 cgtatgggct cccgccgcac cggtattatc gttgtttcaa tgctgattgc gatatttgcc
    49141 gtagggtttg tcgcttcgac attcccgact ggcgcgaata ttctgaccat cattttttat
    49201 aacgtcggcg gtattgttat cttccttggc tttgcgtggt ggaaatacag taaatatata
    49261 aagggattaa cggctgaaga gcgccatatt gaagcgacgc cagccagcaa tgttgattaa
    49321 gcagaataat aaaaaatgag caggcatttt ccctcttcct ggatgaggag agggaaaata
    49381 gtttctgcct ttatattttt ataattacaa cgataaaagg ctgtactttt tctttagctc
    49441 atggattaac acaatgaaat taatcactgc accatgcaga gcattacttg ctctgccgtt
    49501 ttgctacgcc ttttctgcgg caggagaaga agcacgtccg gcagaacatg acgacacaaa
    49561 aacacctgca attacctcga catcttctcc ttcatttcgt ttttacggcg aattaggggt
    49621 tggtggatat atggatttag agggtgagaa taaacataaa tacagcgacg gtacctatat
    49681 tgaaggtggc ctggagatga agtacggctc ctggttcggc ctgatttacg gcgaaggctg
    49741 gaccgtgcag gccgaccacg acggcaatgc ctgggtgcca gaccatagct ggggtggttt
    49801 cgagggcgga attaaccgtt tctatggcgg ttatcgtacc aatgatggca ccgaaatcat
    49861 gctcagtctg cgtcaggatt cctcgctgga tgacctgcaa tggtggggcg acttcacccc
    49921 cgatctgggc tacgtcattc ccaatacccg cgacattatg accgcgctga aggtgcagaa
    49981 cttaagcggc aactttcgtt atagcgtcac cgccacacct gccggacatc atgacgaaag
    50041 caaagcctgg ctacattttg gcaagtacga tcgctatgac gacaaataca cctatccggc
    50101 aatgatgaac ggttacatcc agtatgacct tgccgaaggc atcacctgga tgaacggtct
    50161 ggaaatcacc gacggcacag gacagctcta tctcacgggc ctgctaactc ctaactttgc
    50221 cgctcgcgcc tggcaccata ccggacgcgc cgacgggctg gacgtaccgg gaagtgaaag
    50281 tgggatgatg gtgagcgcca tgtatgaagc gttaaagggc gtttatctct ccaccgctta
    50341 cacctacgcc aaacatcgcc ctgaccacgc tgacgatgaa accacctctt tcatgcagtt
    50401 tggtatctgg tacgaatacg gcggcggacg tttcgccacg gcttttgata gccgcttcta
    50461 catgaaaaat gcctctcacg atcccagcga ccaaatcttc ctgatgcaat atttctactg
    50521 gtaataagga ctgtaatttc atgaaaataa aaactatttt aacgccagta acctgcgctc
    50581 tgctgataag tttttccgcc catgccgcta acgccgacaa ttataaaaac gtgattaacc
    50641 gtactggcgc gccgcagtac atgaaggatt acgattacga cgatcaccag cgttttaatc
    50701 cgtttttcga tctcggagcc tggcatggtc atctgttgcc agacggccct aacaccatgg
    50761 gcggctttcc gggcgttgcg ctgctgacgg aagagtacat caactttatg gccagcaatt
    50821 tcgaccgcct gaccgtctgg caggacggca agaaagtcga ctttacgctg gaggcataca
    50881 gtattcccgg tgcgctggtg caaaaactga cagcaaaaga tgtgcaagtc gaaatgactc
    50941 tgcgcttcgc cacgccgcgc acgtcactac tggaaaccaa aatcaccagc aataaaccgc
    51001 tggatctggt gtgggacggc gaactgctgg aaaaactgga agcgaaagaa gggaaaccgc
    51061 tttccgataa aaccattgct ggcgaatacc ctgactatca gcgcaaaatc agcgccaccc
    51121 gtgatggcct gaaagtcacc tttggcaaag tgcgcgccac ctgggatctg ctgacctccg
    51181 gcgaatcgga atatcaggtg cataaatccc tgccggtgca gactgaaatc aacggtaatc
    51241 gctttaccag taaggcgcat atcaacggtt cgaccacgct ctacaccacc tattcccatc
    51301 tgctgaccgc tcaggaagtt agcaaagagc aaatgcagat ccgcgatatt ctggcgcgtc
    51361 cggcgtttta tctcaccgcc tcgcagcaac gctgggaaga atacctgaag aaagggttaa
    51421 ccaatccgga tgcgacgccg gaacagacgc gcgtcgcggt gaaagcgatc gaaacgctca
    51481 acggtaactg gcgctcaccg ggcggtgcgg tgaaatttaa caccgttaca ccgtcggtga
    51541 ccgggcgctg gttctccggc aatcagacct ggccgtggga tacctggaag caggcgtttg
    51601 cgatggcgca tttcaatccg gacatcgcca aagagaatat ccgcgcggtc ttctcctggc
    51661 agatccagcc tggcgatagc gtgcgtccgc aggatgtggg ctttgtcccc gacctgatag
    51721 cgtggaacct tagccccgag cgtggtggcg atggcggcaa ctggaacgaa cgtaatacca
    51781 aacccagcct tgccgcctgg tcggtgatgg aagtgtacaa cgtcacccag gataaagcct
    51841 ggttggcaga gatgtacccg aaactggtgg cctatcacga ctggtggtta cgtaaccgcg
    51901 atcacaacgg caacggcgtg ccggaatatg gcgcgacccg cgacaaagcc cacaacactg
    51961 agagcggcga gatgctgttt acggtgaaga aaggcaacaa agaagagacg cagtctgggc
    52021 tgaacaacta cgcccgcgtg gtggagaaag gccagtacga cagtctggaa attccggcac
    52081 aggtcgctgc gtcgtgggaa tcggggcgtg atgatgccgc cgtctttggt tttatcgaca
    52141 aagaacagtt ggataaatat gtcgctaatg gcggcaaacg tagcgactgg acggtgaaat
    52201 tcgccgaaaa ccgcagtcag gacggaacgt tgctgggcta ctcgctattg caggagtcgg
    52261 tggatcaggc cagctatatg tacagcgata accattatct ggcggagatg gcaaccatcc
    52321 tcggtaagcc agaagaggcc aaacgctatc gccagttggc acagcagctc gcggactaca
    52381 tcaacacctg tatgttcgac ccgactaccc agttctacta tgacgtacgt attgaagata
    52441 aaccactggc gaacggctgc gcgggcaaac cgattgttga gcgcggtaaa gggccggaag
    52501 gctggtcgcc gctgtttaac ggtgcggcaa cgcaggccaa tgccgatgcg gtggtgaagg
    52561 tgatgctcga tcctaaagag ttcaacacct ttgtcccgct gggaacggcg gcgttaacca
    52621 atccggcttt tggcgctgat atctactggc gtgggcgcgt atgggtggat cagttctggt
    52681 ttggtctgaa agggatggag cgttacggtt atcgcgatga tgccctgaaa ctggcggata
    52741 cgttcttccg tcacgccaaa ggattaaccg ccgatggccc aattcaggag aattacaacc
    52801 cgctgactgg cgcacagcaa ggcgcaccaa atttctcctg gagtgccgcg catttgtata
    52861 tgttgtataa cgattttttc cgtaagcaat aatttgcaac tgctggcgga tgcggcgtaa
    52921 acgccttatc cgccctacat gtgcaatcct cctggccgga taaaacgcga caagcgtcgc
    52981 atccggcatc tgcaccgtgc cactggcgga tgcggcgtga acgccttatc cgccctacat
    53041 gtgcaatccc ccaggccgga taaaacgcgg caagcgtcgc atccggcgtt accaccgggc
    53101 gtattctttt tgaatcccat cacaaacccc gcactcccct tttccctttt ctccggcgac
    53161 ggctaaatta gaactcatcc gaccacataa caattatttt acatactgga caattttatg
    53221 agctacccgt cgctgttcgc cccgctggat ttaggtttta ccacgttaaa aaaccgcgtg
    53281 ttgatgggct caatgcacac cgggctggag gaatacccgg acggtgccga gcggctggca
    53341 gcgttttatg ccgaacgcgc ccgtcacggc gtggcgctga ttgtcagcgg cggcattgca
    53401 ccagatttaa caggcgttgg catggaaggt ggcgcaatgc tcaacgacgc cagccagatc
    53461 ccacaccatc gcaccattac cgaagcggta catcaggaag gcggcaaaat agccctgcaa
    53521 attttgcata ccgggcgcta cagctaccaa ccgcatctgg tcgctccctc cgcattgcag
    53581 gcccccatca accgtttcgt tcctcacgaa ctcacccatg aagagatcct gcaactgatc
    53641 gacgatttcg cccactgcgc gcaactggca cgggaggcag gatacgacgg tgtagaggta
    53701 atggggtccg aagggtattt gatcaacgaa tttctgacgc tgcgcaccaa tcagcgtagt
    53761 gaccagtggg gcggcgatta ccgcaaccgg atgcgatttg ccgtagaagt agtgcgtgcg
    53821 gtgcgcgaac gcgtcggcaa cgacttcatt attatctacc gactgtcgat gctcgacctg
    53881 gtcgaagacg gcgggacttt tgccgaaacg gtagagctgg cgcaggccat tgaagcggcg
    53941 ggcgcgacca ttatcaacac cggcattggc tggcatgaag cacgtattcc gaccattgcc
    54001 acgcccgtgc cgcgcggcgc atttacttgg gtcacgcgca aactgaaagg ccacgtttcg
    54061 ctgccgctgg tgaccaccaa ccggattaac gatccgcagg ttgccgacga cattctctcg
    54121 cgcggcgatg ccgatatggt gtcgatggca cgaccgtttc ttgctgatgc ggaactgctg
    54181 tcaaaagcgc aatcggggcg agccgatgag atcaacacct gcattggctg caatcaggcc
    54241 tgcctcgatc agatcttcgt tggcaaagtc acctcgtgcc tggtgaatcc tcgcgcctgc
    54301 cacgaaacca aaatgccaat ccttcctgcc gtgcagaaaa aaaatctggc ggtggtcggt
    54361 gcgggacctg ctgggctggc gtttgccatt aacgcggcgg cgcgtgggca tcaggtaaca
    54421 ttgtttgatg cccacagcga gatcggcggg cagtttaata tcgccaaaca gatccccggc
    54481 aaagaggagt tttacgaaac gctgcgctat taccaccgga tgatcgaagt gacgggcgtg
    54541 acgctgaaac tcaatcacac cgtgacggcg gatcagttac aggcgttcga tgaaacgatc
    54601 ctcgccagtg ggatcgtgcc gcgcgttccg cctatcgacg ggatcgatca tccgaaggta
    54661 ttgagttatc tcgatgtact gcgcgacaaa gcgccggtag gcaacaaggt tgccatcatc
    54721 ggttgtggcg ggattggttt tgatacggcg atgtatttaa gtcagccggg cgaatccacc
    54781 agccagaata tcgccgggtt ctgtaatgag tgggggatcg acagcagcct acaacaggct
    54841 ggtggcttaa gcccgcaggg aatgcagatc ccgcgtagcc cacggcaaat tgtgatgctc
    54901 cagcgcaaag ccagcaaacc gggacagggg ttaggcaaaa ccaccggctg gatccatcgc
    54961 actaccctgc tctcgcgcgg tgtgaaaatg atcccaggcg tcagttacca gaagatcgac
    55021 gatgacgggc tgcatgtggt gatcaacggc gaaacacagg tattcgcggt ggacaatgtg
    55081 gtgatctgcg cagggcaaga gccaaaccgc gcgctggcgc aaccgctgat tgatagcggg
    55141 aaaactgtgc atttaattgg cggctgcgat gtggctatgg agctggacgc acgacgggca
    55201 attgcccagg gaacacggct ggcgctggag a
//
LOCUS       DAZGVQ010000010        54638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000010 DAZGVQ010000000
VERSION     DAZGVQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.010"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..164
                     /locus_tag="ACHLAG_000570"
     CDS             <1..164
                     /locus_tag="ACHLAG_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFP0653868.1"
                     /translation="QDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFKPFFTNFN
                     TIERIENYL"
     gene            161..640
                     /locus_tag="ACHLAG_000571"
     CDS             161..640
                     /locus_tag="ACHLAG_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFP0653869.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(694..1644)
                     /gene="corA"
                     /locus_tag="ACHLAG_000572"
     CDS             complement(694..1644)
                     /gene="corA"
                     /locus_tag="ACHLAG_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFP0653870.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1796..1852)
                     /gene="ysgD"
                     /locus_tag="ACHLAG_000573"
     CDS             complement(1796..1852)
                     /gene="ysgD"
                     /locus_tag="ACHLAG_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFP0653871.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2014..2778
                     /gene="yigE"
                     /locus_tag="ACHLAG_000574"
     CDS             2014..2778
                     /gene="yigE"
                     /locus_tag="ACHLAG_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFP0653872.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(2925..5087)
                     /gene="uvrD"
                     /locus_tag="ACHLAG_000575"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(2925..5087)
                     /gene="uvrD"
                     /locus_tag="ACHLAG_000575"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFP0653873.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPAEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(5171..5887)
                     /gene="yigB"
                     /locus_tag="ACHLAG_000576"
     CDS             complement(5171..5887)
                     /gene="yigB"
                     /locus_tag="ACHLAG_000576"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFP0653874.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(5887..6783)
                     /gene="xerC"
                     /locus_tag="ACHLAG_000577"
     CDS             complement(5887..6783)
                     /gene="xerC"
                     /locus_tag="ACHLAG_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFP0653875.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTVVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(6780..7487)
                     /gene="yigA"
                     /locus_tag="ACHLAG_000578"
     CDS             complement(6780..7487)
                     /gene="yigA"
                     /locus_tag="ACHLAG_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFP0653876.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(7484..8308)
                     /gene="dapF"
                     /locus_tag="ACHLAG_000579"
     CDS             complement(7484..8308)
                     /gene="dapF"
                     /locus_tag="ACHLAG_000579"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFP0653877.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(8345..8548)
                     /gene="yifL"
                     /locus_tag="ACHLAG_000580"
     CDS             complement(8345..8548)
                     /gene="yifL"
                     /locus_tag="ACHLAG_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0653878.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8707..8849
                     /locus_tag="ACHLAG_000581"
                     /pseudo
     CDS             8707..8849
                     /locus_tag="ACHLAG_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            9011..9331
                     /gene="cyaY"
                     /locus_tag="ACHLAG_000582"
     CDS             9011..9331
                     /gene="cyaY"
                     /locus_tag="ACHLAG_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFP0653879.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(9371..11917)
                     /gene="cyaA"
                     /locus_tag="ACHLAG_000583"
     CDS             complement(9371..11917)
                     /gene="cyaA"
                     /locus_tag="ACHLAG_000583"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFP0653880.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            12304..13245
                     /gene="hemC"
                     /locus_tag="ACHLAG_000584"
     CDS             12304..13245
                     /gene="hemC"
                     /locus_tag="ACHLAG_000584"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFP0653881.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            13242..13982
                     /gene="hemD"
                     /locus_tag="ACHLAG_000585"
     CDS             13242..13982
                     /gene="hemD"
                     /locus_tag="ACHLAG_000585"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFP0653882.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            14004..15209
                     /gene="hemX"
                     /locus_tag="ACHLAG_000586"
     CDS             14004..15209
                     /gene="hemX"
                     /locus_tag="ACHLAG_000586"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFP0653883.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            15212..16408
                     /gene="hemY"
                     /locus_tag="ACHLAG_000587"
     CDS             15212..16408
                     /gene="hemY"
                     /locus_tag="ACHLAG_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFP0653884.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            17088..17225
                     /locus_tag="ACHLAG_000588"
                     /pseudo
     CDS             17088..17225
                     /locus_tag="ACHLAG_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(17333..18568)
                     /gene="aslB"
                     /locus_tag="ACHLAG_000589"
     CDS             complement(17333..18568)
                     /gene="aslB"
                     /locus_tag="ACHLAG_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP0653885.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPLYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(18715..18791)
                     /locus_tag="ACHLAG_000590"
     tRNA            complement(18715..18791)
                     /locus_tag="ACHLAG_000590"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18755..18757),aa:Pro,seq:tgg)
     gene            complement(18834..18920)
                     /locus_tag="ACHLAG_000591"
     tRNA            complement(18834..18920)
                     /locus_tag="ACHLAG_000591"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18884..18886),aa:Leu,seq:cag)
     gene            complement(18941..19016)
                     /locus_tag="ACHLAG_000592"
     tRNA            complement(18941..19016)
                     /locus_tag="ACHLAG_000592"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18981..18983),aa:His,seq:gtg)
     gene            complement(19075..19151)
                     /locus_tag="ACHLAG_000593"
     tRNA            complement(19075..19151)
                     /locus_tag="ACHLAG_000593"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19115..19117),aa:Arg,seq:ccg)
     gene            complement(19254..20639)
                     /gene="yifK"
                     /locus_tag="ACHLAG_000594"
     CDS             complement(19254..20639)
                     /gene="yifK"
                     /locus_tag="ACHLAG_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0653886.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(20830..21570)
                     /gene="wecG"
                     /locus_tag="ACHLAG_000595"
     CDS             complement(20830..21570)
                     /gene="wecG"
                     /locus_tag="ACHLAG_000595"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFP0653887.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(21573..22925)
                     /gene="wzyE"
                     /locus_tag="ACHLAG_000596"
     CDS             complement(21573..22925)
                     /gene="wzyE"
                     /locus_tag="ACHLAG_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFP0653888.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(22922..24001)
                     /gene="rffT"
                     /locus_tag="ACHLAG_000597"
                     /gene_synonym="wecF"
     CDS             complement(22922..24001)
                     /gene="rffT"
                     /locus_tag="ACHLAG_000597"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFP0653889.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDLSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEGA"
     gene            complement(23998..25248)
                     /gene="wzxE"
                     /locus_tag="ACHLAG_000598"
     CDS             complement(23998..25248)
                     /gene="wzxE"
                     /locus_tag="ACHLAG_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFP0653890.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGYTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(25250..26380)
                     /gene="rffA"
                     /locus_tag="ACHLAG_000599"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(25250..26380)
                     /gene="rffA"
                     /locus_tag="ACHLAG_000599"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFP0653891.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(26385..27059)
                     /gene="rffC"
                     /locus_tag="ACHLAG_000600"
                     /gene_synonym="wecD"
     CDS             complement(26385..27059)
                     /gene="rffC"
                     /locus_tag="ACHLAG_000600"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFP0653892.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(27037..27918)
                     /gene="rfbA"
                     /locus_tag="ACHLAG_000601"
     CDS             complement(27037..27918)
                     /gene="rfbA"
                     /locus_tag="ACHLAG_000601"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP0653893.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(27937..29004)
                     /gene="rffG"
                     /locus_tag="ACHLAG_000602"
     CDS             complement(27937..29004)
                     /gene="rffG"
                     /locus_tag="ACHLAG_000602"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP0653894.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(29004..30266)
                     /gene="wecC"
                     /locus_tag="ACHLAG_000603"
     CDS             complement(29004..30266)
                     /gene="wecC"
                     /locus_tag="ACHLAG_000603"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFP0653895.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(30263..31393)
                     /gene="wecB"
                     /locus_tag="ACHLAG_000604"
     CDS             complement(30263..31393)
                     /gene="wecB"
                     /locus_tag="ACHLAG_000604"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFP0653896.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(31449..32495)
                     /gene="wzzE"
                     /locus_tag="ACHLAG_000605"
     CDS             complement(31449..32495)
                     /gene="wzzE"
                     /locus_tag="ACHLAG_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFP0653897.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(32507..33610)
                     /gene="wecA"
                     /locus_tag="ACHLAG_000606"
     CDS             complement(32507..33610)
                     /gene="wecA"
                     /locus_tag="ACHLAG_000606"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFP0653898.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(33850..35109)
                     /gene="rho"
                     /locus_tag="ACHLAG_000607"
     CDS             complement(33850..35109)
                     /gene="rho"
                     /locus_tag="ACHLAG_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFP0653899.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(35194..35295)
                     /gene="rhoL"
                     /locus_tag="ACHLAG_000608"
     CDS             complement(35194..35295)
                     /gene="rhoL"
                     /locus_tag="ACHLAG_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFP0653900.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(35436..35765)
                     /gene="trxA"
                     /locus_tag="ACHLAG_000609"
     CDS             complement(35436..35765)
                     /gene="trxA"
                     /locus_tag="ACHLAG_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFP0653901.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            35896..37161
                     /gene="rhlB"
                     /locus_tag="ACHLAG_000610"
     CDS             35896..37161
                     /gene="rhlB"
                     /locus_tag="ACHLAG_000610"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFP0653902.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            37295..38779
                     /gene="gppA"
                     /locus_tag="ACHLAG_000611"
     CDS             37295..38779
                     /gene="gppA"
                     /locus_tag="ACHLAG_000611"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFP0653903.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(38826..40847)
                     /gene="rep"
                     /locus_tag="ACHLAG_000612"
     CDS             complement(38826..40847)
                     /gene="rep"
                     /locus_tag="ACHLAG_000612"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFP0653904.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            41064..41513
                     /locus_tag="ACHLAG_000613"
     CDS             41064..41513
                     /locus_tag="ACHLAG_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP0653905.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTKYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            41712..41993
                     /gene="ppiC"
                     /locus_tag="ACHLAG_000614"
     CDS             41712..41993
                     /gene="ppiC"
                     /locus_tag="ACHLAG_000614"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFP0653906.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(42080..43555)
                     /gene="ilvC"
                     /locus_tag="ACHLAG_000615"
     CDS             complement(42080..43555)
                     /gene="ilvC"
                     /locus_tag="ACHLAG_000615"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFP0653907.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            43705..44595
                     /gene="ilvY"
                     /locus_tag="ACHLAG_000616"
     CDS             43705..44595
                     /gene="ilvY"
                     /locus_tag="ACHLAG_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000365800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFP0653908.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QALFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(44592..46136)
                     /gene="ilvA"
                     /locus_tag="ACHLAG_000617"
     CDS             complement(44592..46136)
                     /gene="ilvA"
                     /locus_tag="ACHLAG_000617"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFP0653909.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(46139..47989)
                     /gene="ilvD"
                     /locus_tag="ACHLAG_000618"
     CDS             complement(46139..47989)
                     /gene="ilvD"
                     /locus_tag="ACHLAG_000618"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFP0653910.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(48054..48983)
                     /gene="ilvE"
                     /locus_tag="ACHLAG_000619"
     CDS             complement(48054..48983)
                     /gene="ilvE"
                     /locus_tag="ACHLAG_000619"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFP0653911.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(49003..49266)
                     /gene="ilvM"
                     /locus_tag="ACHLAG_000620"
     CDS             complement(49003..49266)
                     /gene="ilvM"
                     /locus_tag="ACHLAG_000620"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFP0653912.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(49263..50909)
                     /gene="ilvG"
                     /locus_tag="ACHLAG_000621"
     CDS             complement(49263..50909)
                     /gene="ilvG"
                     /locus_tag="ACHLAG_000621"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFP0653913.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAAAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(50912..50962)
                     /gene="ilvX"
                     /locus_tag="ACHLAG_000622"
     CDS             complement(50912..50962)
                     /gene="ilvX"
                     /locus_tag="ACHLAG_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFP0653914.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(51049..51147)
                     /gene="ilvL"
                     /locus_tag="ACHLAG_000623"
     CDS             complement(51049..51147)
                     /gene="ilvL"
                     /locus_tag="ACHLAG_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFP0653915.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            51500..53020
                     /gene="yifB"
                     /locus_tag="ACHLAG_000624"
     CDS             51500..53020
                     /gene="yifB"
                     /locus_tag="ACHLAG_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFP0653916.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVVGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(53045..53383)
                     /gene="maoP"
                     /locus_tag="ACHLAG_000625"
     CDS             complement(53045..53383)
                     /gene="maoP"
                     /locus_tag="ACHLAG_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFP0653917.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            53502..54341
                     /gene="hdfR"
                     /locus_tag="ACHLAG_000626"
     CDS             53502..54341
                     /gene="hdfR"
                     /locus_tag="ACHLAG_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFP0653918.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(54437..54512)
                     /locus_tag="ACHLAG_000627"
     tRNA            complement(54437..54512)
                     /locus_tag="ACHLAG_000627"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54477..54479),aa:Trp,seq:cca)
     gene            complement(54521..54597)
                     /locus_tag="ACHLAG_000628"
     tRNA            complement(54521..54597)
                     /locus_tag="ACHLAG_000628"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54561..54563),aa:Asp,seq:gtc)
BASE COUNT        13406 a        14751 c        13835 g        12646 t
ORIGIN      
        1 cacaggacca cttcggtctt gatgtaactg atatcaaaaa caagacttac agcaaatata
       61 gattttttcg catatggttc tttttacaac ggcataaaga ctttgctttc aaaccttttt
      121 tcaccaattt taacactatc gaaagaatcg aaaattatct atgaaaagca aactccagaa
      181 aattatatta tgtttatttc ttctatgctg catctataat ttatggacat tgcgtcctgt
      241 acagattttg tatacatact ctgatgctgg caattcagtc tttctggtcg ttgatcatct
      301 gccatggact gacagcgata aaataaattg gtatctgaag catcagaatg aaattaaaaa
      361 tcaacatccc ctaccagaag gtagttggca tacctggtat gtcattgata tcggtaatgg
      421 cttcactgac tataaaaaat atatcgaagg gccgtatgaa gatctctact gtttcccgac
      481 aattaagagc aatgataatt gcatcgtgaa aaattactta atggttatta atgaataccc
      541 ttatcgtaac actcatattg gtattaacga ttttactgag tatcaattaa cacaagaaaa
      601 taaaatagag cgagttttca atccgcatga ctttaaataa gacaattttt agaaataagc
      661 cggataaaaa aatagcttta tccggcactc atattacagc cagttcttgc gcttaaagta
      721 cagatacggt gccaggcccg cgaggatcat aaagataatc gcgcctgggt agccgaaact
      781 ccacttcagt tctggcataa actcaaagtt catcccatag ctggaagcaa cgagcgtcgg
      841 cggcaggaat accacggata ccaccgagaa aattttgatg atgcggttct gctcgatgtt
      901 gataaaaccc attgccgcct gcatcaggaa gttcaccttc tggaacaggg attcgttatg
      961 cggcagcagg gattcgatat ctcgcaggat ttcacgcgcc tgctccagtt gcccacccgg
     1021 taaacgcgcc ttgcgtacca ggaagttgag cgcacgctgg gtatccatca gacacaggcg
     1081 aactttccag ccgatatctt ccagttccgc cagcgtggag agcgcctcat cgtactcatc
     1141 gccctgatgc ccttccataa ttacccggct caactgctcc agatcgctgt agatattttc
     1201 aatttcatct gccagctgtt cgattttggt ttcgaacaga tccaacagca gctcgtaggc
     1261 gttaccgtcc accattgact ggctacgggc gcgcatacga tacagacgga atgcaggcag
     1321 ttcacgctca cgcagagtaa acagacgacc atcacggatg gtaaatgcca cagtggagtt
     1381 accggcgtga tcttccgcat cttcaaagaa gaagaaggag tgaatatgca ggccgtcatc
     1441 gtcttcaaag aaacgtgccg atgcttcgat gtcttccagt tccgggcggg ttgccaggct
     1501 ctggccaagt tcagattgta cgcgcagtcg ctcgtcgtcg tccggttcga caagatcaat
     1561 ccatactgca tttacaaggg gttgtgactc ttcgacttcc agccgggtca gtcggttatt
     1621 ttccagttga aatgcgctca gcatgaccgg gactcccaat gcttaaaata tcggacagtt
     1681 cggtgggcac acagaaacaa attgggtttc agaccattaa acagcctgac tcagcgcgac
     1741 gggaaaaatt gaggtcgctg acaaccgcta aggctatcag caaaaaggga tagccttagg
     1801 agttgatcct ggatgacagg atagtgagcc agtatctact gggtgtgtcc aaggcgaatg
     1861 tcctcttaga gtgatcgtgc gcgcatgtta cgccagcaaa attttgccgt caacacgcaa
     1921 cagaacacca cagaacaata gttgaacttc gcgctttttg gtactcgtca aggtcgctaa
     1981 caggataaaa aaatccaaca tccaggacct ataatggcgc atcggctact cattggtaaa
     2041 ggaatgatca ccttgaatct caaacggatt tttctcgccc tcaccttact tcccttgttt
     2101 gctgttgccg ctgatgattg cgcactctca gatccgacgc tgaccgtaca ggcgtatacc
     2161 gttaatcctc aaacagagcg ggtgaaaatg tactggcaaa aagccaatgg cgaagcgtgg
     2221 ggaactttac atgctctgct ggcggatatg aatagtcagg gtcaggtgca gatggcgatg
     2281 aacggcggca tctatgatga aagctatgcg ccgctcggtt tgtacatcga aaacggtcag
     2341 cagaaggtgg cgttaaatct cgcttcaggt gaagggaatt tctttatccg tcctggcggc
     2401 gtgttttatg tcgcgggaga taaagtcggc atcgttcgtc tggatgcctt caaaaccagt
     2461 aaagagattc agtttgcggt gcagtcaggg ccaatgttga tggaaaacag tgtaattaat
     2521 ccgcgtattc atcccaacgt cgcctcacgt aaaattcgta acggtgttgg gattaataaa
     2581 catgggaatg ccgtgttttt gttgagccag caggcgacaa atttttatga ttttgcctgt
     2641 tatgccaaag cgaaactgaa cgttgagcag ctgctttatc ttgatggcac gatttcacat
     2701 atgtatatga aaggcggggc aatcccgtgg caacgctatc cttttgtcac catgatttcc
     2761 gtagagcgga aaggttaaaa cgatagtttt gtgtcagata ctgaagatgg cgcagatgcc
     2821 ggatgcggcg taaacgccct atccggccta catgacgttg caatttattg aatatgaatg
     2881 attttttagg ccaaataagg tgcgcagcac cgcatccggc aacgttacac cgactccagc
     2941 cgggcgtatg ccgccaccag ccatttaata ccctggccct ggaatgccac ctgcaaacgg
     3001 ctatgctcgc cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgt
     3061 acacgctgac cgagcttgta gccgctgtcg ttctcgacca tcggcgtccc catccgttga
     3121 tggctgaccg gacgacttac cgtagcgcgc aggcgcacct cttccacaca ctcttccggc
     3181 agctcgccga taaagcgcga cgggcgatgg taaacctctt taccatacag acggcgggtt
     3241 tccgcgtagg tcagcgtcag tttctgcatc gcgcgggtta caccaacgta ggccagacgg
     3301 cgttcttctt ccagacgccc gccttcatcc agcgacatct ggcttgggaa cataccctct
     3361 tccataccga caataaacac ctgcgggaac tccaggcctt tcgccgagtg cagcgtcatc
     3421 aactgcactg catcctgcca ggtatccgcc tgcccctcgc ccgcttccag cgccgcatgg
     3481 gagaggaatg cctgcagcgg cattaaatct tcatcttctt cgttgtagct gaactggcgc
     3541 gttgccgtca ccagttcctc taagttttcg atacgcgtct ggcctttttc gcccttctcc
     3601 tgctcgtaca tggtgcgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc
     3661 ggcatatcgg cagtttcttg cgctaaggca tcgattaatt ccataaatcg ctgtaaggcg
     3721 ctggcagcac gtccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt
     3781 gttaactggc gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc
     3841 cgcgttggcg tattcaccac acgctcaaag gccgcgtcgt cgttacggtt ggcaatcagg
     3901 cgcagatacg agagcgcgtc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa
     3961 atacggtacg gcatactggc ctgcaacagt gcttcttcca gtacacgcga ctgggcgtta
     4021 ctgcggtaga gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg
     4081 cggttaacca caaaacgcgc ttcatcgagt tcgttaaaag cgcaatagag ggaaataggc
     4141 tcaccgtccg cgccatcggt ccacagtttt ttacccagac gcccgttatt gttttcaatc
     4201 agggcgttag cggcgctcag aatattgctg gtagagcggt agttttgctc cagacgaata
     4261 gtttcggcac cggggaaatc attaaggaaa cgctgaatat tctccacctg cgccccgcgc
     4321 cagccgtaga ttgactggtc gtcatcaccg acgatcatca ctttgccggt gtcgcccgcc
     4381 agcaggcgga tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggata
     4441 ttggtaaaac gttcgcggta gtgttgtagg atatgcggct tgttaagcca cagttcgtga
     4501 gcgcgcagca gcagctcggc gaagtccacc aggcccgcgc ggtcacacgc ttcctgatac
     4561 gcctgataca ccttctgcca ggtctgctcc gccggattac cgtagctttg aatatgatgc
     4621 ggacgcaggc cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac
     4681 tgcttctcgt cgaggttcat ggctttgatc agacgcttaa gcaggcgcag ctggtcctcg
     4741 ctatcgagga tctggaaatc ctgcggcaga ttggcgtcca tatggtgcgc acgcagcagg
     4801 cggtgcgcca gcccgtggaa ggtgccgacc cacatgccgc cctggctggt gcccatcagt
     4861 tgcccgatac gatgacgcat ctccgccgcc gctttgttgg taaacgtcac cgccataatc
     4921 gagtatggcg agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc
     4981 gtcttaccac tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct
     5041 tcgcgctgtt tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg
     5101 taaaaatagg ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca
     5161 gatttgctga ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgcggca
     5221 gtaaacggct gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaggcct
     5281 gcattccgct gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga
     5341 tctcgccgat cggcacgttg agtttttccg cagccagaaa gtacatatcg ctgaacggtt
     5401 ttgagcgccc gtgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca
     5461 gttccggctg ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt
     5521 ttaaggtgtc gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttga
     5581 tcattgctgc gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg
     5641 cttgttcaat cgaacgaaaa cgccaacgcg tcacgtcgtg ataaatctcg ggttccgctt
     5701 cccgtaccgc ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat
     5761 gataattttg cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat
     5821 cgtaaagggt atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa
     5881 aacgcattat ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg
     5941 ttgaaaatca agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagtagctc
     6001 ctgcacacca cgaagatcgc cgctcgactc cagcatatgc gtagcgaacg agtgacgtaa
     6061 tttatgtggg tgaacgtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt
     6121 ctgcacatta cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc
     6181 gctaccaaac aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc
     6241 aatcggcagg cggcgctctt tgctgccttt ccccataacc cacacttcac cagactccag
     6301 gtcgaggtgt ttaatatcca gccccaccag ctcagaaaga cgcagacccg cgccgtacat
     6361 cacttccagc attgcacggt cgcgtacagc gaggggatca ttgatatcaa tatccagcag
     6421 ccgattcata tcgtcgacgt cgatgttttt cggcagatga cgcggcgctt tcggtgccga
     6481 aacacctttc gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct
     6541 acgtagcgca gaaagccgta acgccagact tgctgctccc agccctttac ggcgactgcg
     6601 tacagcaaaa ttgcgcacca ccgtcacatc acattgctgc cagctttgca ggccgttttc
     6661 gctggcaaaa ttgatgatcg cctcaagctg acgctggtag ttaagcaggg ttatcgggct
     6721 aagctggcgc tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt
     6781 catacgcgtt caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt
     6841 aactgcgttc cttgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacaaca
     6901 cccaaatcag catcgcttcc cagcatcgac atcgccaccg atcccactgc tttcgcttca
     6961 ggtagcacca ccagcagctc aggtccgtta agcggcccaa gatagtgctg ttcctgcccc
     7021 aaacgctgaa tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc
     7081 gacggcgcac ctaagcgcca gcgatccgga aacaggcgca gactcgcacc tgccaggccg
     7141 agatcgcgcg cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg
     7201 agactgcgct gcaggtagag taggcgataa aacaggcctt cgttggcgat agcctgttcc
     7261 atcaacagcg ccatgttctc ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc
     7321 cactcgacca acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg
     7381 cgcgcattac ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca
     7441 tcaagctccg tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc
     7501 cgtcgtagac atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg
     7561 cgatatcaag acgaccgccg gggagttcca cgcgtacttc ttcggccagc aaaccttgct
     7621 gaatccctac tgcaaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc
     7681 cacgctcata aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat
     7741 tggcgcgctc cggaaaacgc tcgtggcttt ccagaacagg accaagcgtt tctaccgccg
     7801 cggtatcgac atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc
     7861 ataagattgt ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa
     7921 acggcacggc ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat
     7981 cggtgacggt cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg
     8041 tcagtccttt cagacgcaca aaacgggcaa agcagcgcgc accgttgccg cactgcgcca
     8101 cttcactgcc atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg
     8161 gctcaaccac cagcagttgg tcaaacccta cccccaggtg ccgatcagcc aggcgacgaa
     8221 tcagctccgg tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc
     8281 caaggccatg cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg
     8341 acttttagta attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca
     8401 ccgtggattg cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag
     8461 gcgggaaata gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata
     8521 cagtgagtgc cttaaacacg tttttcatta tggattgcct gtaaagttca tcgctttctg
     8581 ctttctatca tcgcaggaga agacgtaaaa gcaagcgttt agcgactctt tatgaagtct
     8641 gaaaagtgag gaaatgctgc gagtataacg cttactcgtg gggatttatg ttttgctggt
     8701 agtttcgtga tcggcacctc gcgccaggat ggctttatgc actatcgcaa tgtagtttgt
     8761 ttgttggctt ttttagttcc gtcagcgtga ggatgctcgc tcggtaaacc tgatcataat
     8821 gtttaccaga aacagggaaa ggcgtggtgt aactgcgccc taatgaagag acaaatcttt
     8881 cgctacactg agacatcatt attccggtga cggatgaatc gtgccggaac gcagccgcag
     8941 aggtgcaaac tttgaagccc tgaccccatg ccgctatact gccgccatca cttaaaaaca
     9001 ggatacaaca atgaacgaca gtgaatttca tcgcctggct gatcaactct ggctgaccat
     9061 tgaagaacgc ctggacgact gggatggcga cagcgatatc gactgcgaaa tcaacggcgg
     9121 cgtactgacc attacctttg aaaacggcag caaaatcatt attaaccgcc aggagccgct
     9181 gcaccaggtg tggctggcaa ccaaacaggg cggctaccat tttgacctaa aaggcgatga
     9241 atggatttgc gatcgcagcg gcgaaacctt ctgggatttg ctggaacagg cggcgacaca
     9301 gcaagcgggt gaaacagtca gtttccgcta agattgcatg ccggataagc ctcgctttcc
     9361 ggcacgttca tcacgaaaaa tattgctgta atagcggcgt atcgtgatcc tgattggcag
     9421 gcggcatgtt accgatagat tttgtgcgga acggaatcac ctgttcacga ccatcaacct
     9481 tcacaatctg atagaactgc ggcaggttga agttgatgaa gcttgagccg taggtgaagc
     9541 ggtcgtgcga tgacgagtag aagcgactga cgtcacgcac cagttcctct ttgctgcctt
     9601 cgcagtggtg atacacctca acccggttgc tttcgtcgag aatgtagata ttaaagccat
     9661 tctcgtcttg cgtttcttcg aagaagaact ggatgatccc ttcactagca aagccgtcca
     9721 ccaccgccgg taatttgacg tgattggttt caacctgcac tgacaagccg tgcagtttgt
     9781 tatgcgaaat agcgccataa aactcgatgg cgttttccag tttttgtacc gatacattca
     9841 ggcgttcgaa gaacaacccc caggtttgac cagaaacgcg cagcgccttg aaacgcccgg
     9901 tttcctggcg ggtgctggaa aggcgcagtt caatacactc agaaaccagt tgctgcacgc
     9961 gagtacggat taacccgcgc agatgctggc tataacagaa gacttccacg ctatctggcg
    10021 gtgcggcgtc ctgatgcatt ttgccgagaa tagttttcag ggcttcgatc atcgattgct
    10081 cgccgttgaa gtgcagcgta cgcacttcgt tccacgagtt gcggtacagc aggtcaacgc
    10141 tacctaccag gcaattttga ttctcgccaa agctgaagac atccagctta cggaaatcaa
    10201 aatgcaccac ctgattgcgg aacgccgctg tcgggtcata ttccaggtta acgataatcg
    10261 ccagatggcg gatctcacac gggctgtaga gcgccttcgg tgtcggtgca ggtaagcgca
    10321 gcgggaaatg gtgcgacacg tcggcgacca tctcctgcaa cttaggcaaa tcgacaatgc
    10381 cgttaccttt aatatacaaa cgggtgcgcg aggtcagcag gccgttaaac catgcccacg
    10441 ccaccagttt gttcaggtaa cggttatatt ccagcggctg atggctgatg atcgactcaa
    10501 tatttggcgc gcggttatac agataccaac ctgaacggtt agcacggccc ggcggcacat
    10561 aaataaaggt cagattcggt tccgagagat cgggtgaaat ctgcgggttt accagcgtca
    10621 ctttacctgg taatgcttca aacgcggcat acagcttacg cgtcagcacg ccgatatcct
    10681 gcggactggc ggagacgcta aggttattgc gacgcgcaaa gcggatcaga ttacggtagc
    10741 tctgcatcat cgcgtcgagc aactcgttgt gcgcctcacg cacctgatca atcttccagt
    10801 tagcgcggtt atcgagcatt gccagacgag cttcgtccca accccactcg ctcactaact
    10861 ggctcaacac tgcgcgacgc cagcctacgc aggcgcgttc acggctgagc ttttcgcaca
    10921 cttttaaata gaagcagcga cgtactaaat ccagacgggt aaaatcttca atcgccgtca
    10981 ggtattcagt aacacgctcc agcatcatgc agtatggatc gagaccaaac gatacaatct
    11041 cgccgtcgtg caaacgctgt ttgatatctt tcgccagcag acgtgggttc gggtattccc
    11101 aggaataggc ttccagcagc agtgttttca gtaccgcttt gtatggggaa tcgatactct
    11161 tgtagagctg ccaaaggctg gcaccaaagt actcttcagc agaaagcgag cttaagccac
    11221 cgagatccag ccattcattt ggcgtcagca cgccctgcgc gtaaagcgtc atcacatagt
    11281 cgtcgtaatg ctcttcttcg tcgcacggca ccatattcca cagaatacgc ttaccggcga
    11341 gacgcacggc ggtacgataa aattcgtcaa gcagcagtat atgctgggtg gagccacaat
    11401 cttcgccccc caggctgccg ctttcattat gacggaagcg gttttcatca atcaggaaga
    11461 agctgacttc cacacccagc gaggcggccc agctttccag cagactacat ttacgttgta
    11521 gtaactggcg ctcttcgcta tcgagccagg attgatgaca gacccagata tccaggtcag
    11581 aggaacaact ttgccctacg gacgaggtgc tgcccatggt gtatacacca gtaattggaa
    11641 gctcaccttt cggtgggtcc tgtactgaca ttccacgata cagttcaagc tcgttcaggt
    11701 agtggcgttg agtttcatca ggcgtgtaaa ggcaaatgcc tttgggaacg ttaccatcaa
    11761 ggtaacccgg cattagcgga tggtgatagt gcaacaatgt cggcagtaga ctgtagacct
    11821 gttggaatgc aggccccata gcagcaagcg cgcgatccac acgcaattga tttatggcat
    11881 ccagtctctg tttcagagtc tcaatataga ggtacaagac gtatcgcctg atttgctacc
    11941 cgtcatgact gtgattccgc caacatcaac ggtaacacgc ggcattcggg atatttcgta
    12001 tgtcaaaggt aaccgttacc acttttcgcg cctggttttt ttagtttcac gacgaaaaaa
    12061 tggtctaaaa cgtgatcaat ttaacacctt gctgattgac cgtaaagaaa gatgcgctac
    12121 atacaagtgt agcaccgttt attctctgta aattccttat tacgacggcg tgaaacgcct
    12181 gtcaggatcc actgccagac ctcattttac ggtttgcgca ggcgtctacg tttcaccaca
    12241 acactgacat cactctggca aggatgttag gatggaccac ggatgataat gacggtaaca
    12301 agcatgttag acaatgtttt aagaattgcc acacgccaaa gcccacttgc actctggcag
    12361 gcacactatg tcaaagacaa gttgatggcg agccatccgg gcctggtggt tgaactggta
    12421 ccgatggtga cgcgcggcga tgtgattctt gatacgccgc tggcgaaagt aggcggaaaa
    12481 ggcttatttg taaaagagct ggaagtcgcg ctcctggaga atcgcgccga tatcgccgta
    12541 cattcaatga aagatgtgcc ggttgaattc ccgcaaggtc tggggttggt cactatttgt
    12601 gagcgtgaag atcctcgcga tgcctttgtg tccaataatt atgacagtct ggatgcgtta
    12661 ccggcaggca gtatcgtcgg gacgtccagt ttacgtcgcc agtgccaact ggctgaacgc
    12721 cgcccggatc tgattatccg ctccctgcgc ggcaacgtcg gcactcgcct gagcaaacta
    12781 gataacggcg aatacgatgc catcattctt gccgtagccg gactaaaacg tttaggtctg
    12841 gagtcccgca ttcgcgccgc gttaccaccc gagatttctc ttccggcggt aggacaaggt
    12901 gcagtcggta ttgaatgccg ccttgatgat acgcgcactc gcgagctgct tgccgcgctg
    12961 aatcaccacg aaactgcact gcgcgttacc gcagaacgcg ccatgaatac ccgtctcgaa
    13021 ggtggatgtc aggtgccaat tggtagctac gccgagctta ttgatggtga aatctggctg
    13081 cgtgcgctgg tcggcgcgcc ggacggttcg cagattattc gcggtgaacg ccgcggtgcg
    13141 ccgcaaaatg ccgaacaaat ggggatttcg ctggcagaag agctactgaa taacggcgcg
    13201 cgcgagatcc tcgctgaagt ctataacgga gacgccccgg catgagtatc ctggtcaccc
    13261 gcccgtctcc cgctggagaa gagttagtga gccgtctgcg cacactgggg caggtggcct
    13321 ggcattttcc gctgattgag ttttctccgg gtcgacaatt accgcaactt gctgatcaac
    13381 tggcggcgct gggggagagc gatctgttgt ttgccctctc gcaacacgcg gttgcttttg
    13441 cccaatcaca gctgcatcag caagatcgta aatggccccg actacctgat tatttcgcca
    13501 ttggacgcac caccgcactg gcgctacata ccgtaagcgg acagaagatt ctctacccgc
    13561 aggatcggga aatcagcgaa gtcttgctac aattacctga attacaaaat attgcgggca
    13621 aacgtgcgct gatattacgt ggcaatggcg gtcgtgagct aattggggat accctgacag
    13681 cgcgcggtgc tgaggtcact ttttgtgaat gttatcaacg atgcgcaatc cattacgatg
    13741 gtgcagaaga agcgatgcgc tggcaatccc gcgaggtgac gatggtcgtt gttaccagcg
    13801 gtgaaatgtt gcagcaactc tggtcgctga tcccacaatg gtatcgtgag cactggttac
    13861 tacactgtcg actattggtc gtcagtgagc gtttggcgaa actcgcccgg gaactgggct
    13921 ggcaagacat taaggtcgcc gataacgctg acaacgatgc gcttttacgg gcattacaat
    13981 aactctcata acaggaagcc ataatgacgg aacaagaaaa aacctccgcc gtggttgaag
    14041 agaccaggga ggccgtggac accacgtcac aacctgtcgc aacagaaaaa aagagtaaga
    14101 acaataccgc attgattctc agcgcggtgg ctatcgctat tgctctggcg gcgggcgtcg
    14161 gtttgtatgg ctggggtaaa caacaggccg tcaatcagac cgccaccagc gatgccctgg
    14221 ctaaccaact gacggcattg caaaaagccc aggagagcca aaaagccgag ctggaaggca
    14281 ttattaagca acaagctgca cagcttaagc aggcgaatcg tcagcaagaa acgctggcaa
    14341 aacagttgga tgaagtccaa caaaaggtcg ccaccatttc cggcagcgat gctaaaacct
    14401 ggctgctggc tcaggccgat tttctggtga aactcgccgg acggaagctg tggagcgatc
    14461 aggacgtcac gaccgctgca gcgttgctga aaagtgcaga tgccagcctg gcggatatga
    14521 atgacccgag tctgattacc gttcgtcggg caattaccga tgatatcgcc agcctttctg
    14581 cagtttcgca ggtggattat gacggcatca tccttaagct taatcagctt tcaaatcagg
    14641 tagataacct gcgtctggcc gataatgaca gcgatggttc gccgatggat tccgacggtg
    14701 aagagctttc cagttccatc agcgaatggc gtatcaatct gcaaaaaagc tggcagaact
    14761 ttatggacaa cttcattacg attcgccgtc gtgatgacac cgccgtaccg ctgttagcgc
    14821 caaatcagga tatctatctg cgcgaaaata ttcgctctcg cctgctggtc gcagcacaag
    14881 ctgtaccgcg tcaccaggaa gagacttatc gccaggcgct ggagaacgtc tccacctggg
    14941 tacgagctta ctacgatact gatgatgcca ccaccaaagc gttcctcgac gaggtggacc
    15001 agttaagcca gcaaaatatc tcgatggatc ttccggaaac cctgcaaagc caagcgatgc
    15061 tggaaaaact gatgcagacc cgcgtgcgta acctgctggc acaaccggca gcggggacaa
    15121 cggaagctaa acctgcacct gcacctgcac ctgcacctgc acctgcaccg caaggtgata
    15181 ctccggcagc cgcgccgcaa ggagaataac catgctaaaa gtgttattac tctttgtttt
    15241 gctgattgcg gggatagtgg ttggcccgat gattgccggt catcagggtt acgtgctgat
    15301 ccagactgac aactacaata tcgaaaccag cgtcacgggc ctggcgatta ttttgattct
    15361 ggcgatggta gtgctgtttg ccattgagtg gctactgcgg cggatcttcc gcacaggcgc
    15421 acacacccgt gggtggtttg tcggacgtaa gcgtcgccgt gcccgtaagc agaccgaaca
    15481 ggcgctgctg aaactggcgg aaggcgatta tcagcaagtt gaaaagctga tggcgaaaaa
    15541 tgccgatcac gcggaacaac cggtggtgaa ctatctactg gctgccgaag cagcgcaaca
    15601 acgtggtgat gaagcacgcg ccaaccaaca tctggaacgc gcagcagagc tggccggcaa
    15661 tgacaccatt ccggtagaaa tcacccgtgt acgtctgcaa ctggcccgta atgaaaacca
    15721 tgctgcacgc cacggcgtgg ataagctgct ggaagttacg ccacgccatc cggaagtatt
    15781 acgtctggcg gaacaggcgt atatccgcac aggtgcatgg agttctctgc tggatatcat
    15841 cccatcaatg gcgaaagccc atgttggtga tgaagaacat cgtgcaatgc tggaacaaca
    15901 ggcatggatt ggcctgatgg atcaggcgcg tgccgataac ggtagtgaag gtttgcgtaa
    15961 ctggtggaaa aaccaaagcc ggaaaacgcg tcatcaggta gcgttgcagg tggcaatggc
    16021 ggaacatctt attgaatgtg acgatcatga tactgcccag caaattatca tcgatggcct
    16081 gaaacgccag tacgacgatc gcctactgct gccgattcct cgactgaaaa ccaacaatcc
    16141 ggaacagctg gaaaaagtgc tgcgccagca gatcaaaaac gtcggcgatc gcccgctgtt
    16201 gtggagcaca ctgggccagt cactgatgaa gcacggagaa tggcaggaag catcgctcgc
    16261 cttccgcgcg gcgctgaaac aacgtccgga cgcctacgat tacgcatggc ttgccgacgc
    16321 gctggacaga ctgcacaagc cggaagaagc tgcagcgatg cgtcgcgacg gtttgatgtt
    16381 aacgttgcag aacaacccac cacagtagtt ccttctcacc cggaggcaag cacctccggg
    16441 acctttctga tgcataaaaa aacgcctgct cttattacgg agcaggcgtt aaaacaggtc
    16501 tgtatgacaa caagtgggtg cttcactcaa cgttgtgtcc atggtgtctg atgaggcata
    16561 agcgacatct gtcagtggac gataagcacc gtaaacggct ctgcgtcatt ccggagttta
    16621 tgaggcacta aggcgaacat aagagatgga atgagcatct acccgtttat tatgccacag
    16681 agaatcggga aataacatcc cttaacactt gttatgagat aattctgtaa tcctctttgc
    16741 ttcctgagta ataacttcct gagtgaatat ttaacctgag cttgatccta cacatactta
    16801 ttatgaatga taaaattcat tcaattaata acacatatat taattgccgt taaaactaaa
    16861 aaaaagcatc aataatcaac gcgatataat aaacctgcct tacatatcaa ctgcgccaga
    16921 ggtaggattg aaaacgctct cctgattttc caattcattt tctggataaa taaataattt
    16981 atttttgtca ctattattta tgtaatcatc ctgtcaggga gagggatctc aattatcaat
    17041 gcttaattac gtcatcattt tgatgacatg aaaccggagc attttgcatg gaattttcgt
    17101 tttcacccaa acttcttgtt gttgctgtcg ccgccgctct tcctctcatg gccagcgcag
    17161 cagatacccc gtcaactgcc accgcacgca aaggctttgc cggatacgat cacccaaacc
    17221 agtatccaca gaacgctgca atttattgaa tttgcacgat catgtaggcc ggataaggcg
    17281 tttacgccgc atccggcaat caaccgcagg cggccacaaa atggccgctt ttttacttat
    17341 tcaccaccag caaatgtgcc tgcataatgt cactggccgg gcgaccatgc gccagcaaat
    17401 cagccattgc tttaagatat agcggtagat ggcggaaata acgctgatac ccggcacaca
    17461 aataattcag ccccggtttg ccgctggcat cgagcatgaa gcggtgtttc gggcagcctc
    17521 cccagcacgc ttttaacacg ttacaactgc gacactgcgc cggtaattgc ttaaatttat
    17581 cttcaccaaa cacctgctgt tgcggggaat cgatcatttc tgcaattgtt tgctgatgca
    17641 tattccccag ccgatattgc ggataaacat agtgatcgca ggcgtaaacg tcgccgttgt
    17701 gctcaacaat caccgagcgc ccacaggttg gctgatgatg gcaaaccgca cccggcgcac
    17761 cgacaaaatt ggcaaacgcc cattcgatat tcatcacgaa aatcttgccg acgtcgcgtt
    17821 tgatccagtg gtcgaatatc gccaccagaa attcaccaaa ttcatcgggg cgcaccgacc
    17881 attccgtcag ttccccctga atatcaccag gcgcatgtag tttcagtcca tcgctggcag
    17941 ctgtttcatc agccaggcgc tcgaccaccg gaataaactg gatgaattcg actcccgcat
    18001 cgcacaaaaa atcataaacc tgcaacggtt gctgcgcgct ggtgcggttg acgcagacca
    18061 gcacgttata gtcgacatga tgtttttgca gcagcgtcag ggcacgcatc accagcttat
    18121 gcgttggtct gccacctttg gtcacgcgat attgattgtg gatctcagcc ggaccgtcca
    18181 gcgataaccc aacaagaaaa tgattttctg ccagaaatgc acaccattta tcatcgagca
    18241 gcacgccgtt agtctggaag ctgttactta tcttcctgcc agcaccgtat ttcgcctgta
    18301 actttacggc acggcggtaa aaatccagcc cgagtaacgt tggttcgccg ccctgccagg
    18361 taaaagccac ttcgttttgc ggttcgctgg cagcgatata gtgacggaca tacgcttcca
    18421 gcgtgtcatc gtccatatgc gtgactggct tttcgcggta aagggattgt ttttcgagat
    18481 aaaaacagta gtcacagttc agattacaat cggaaccact cggtttcgcc atcacatgaa
    18541 aagcacgcgt tggaacctgt tgcagcatgg tcactccttg cctgaagtca tccttcaata
    18601 cgttaagggc gaaatcatac cgtggggcaa tgagttatta cgggcgttta ttgcgaaggg
    18661 agggtgacga aatgcacaga aaacaaaaaa ccccgccgaa gcggggttca aaattggtcg
    18721 gcgagagagg attcgaacct ccgacccact ggtcccaaac cagttgcgct accaagctgc
    18781 gctactcgcc gaaatactgc tttttgaatt tttagttcaa ttctttaaag tcgtggtgcg
    18841 agggggggga cttgaacccc cacgtccgta aggacactaa cacctgaagc tagcgcgtct
    18901 accaattccg ccaccttcgc attgtcacaa cttctaataa tggggtggct aatgggattc
    18961 gaacccacga caactggaat cacaatccag ggctctacca actgagctat agccaccact
    19021 acaaatcttg ttacgcggta ttactaccac cgcagctcaa gcgccgggac taaatggcgc
    19081 gcccgacagg attcgaacct gagacctctg cctccggagg gcagcgctct atccagctga
    19141 gctacgggcg cttagcgccg ttgcgggggt ggataatacg gacttcccac ccctctgtct
    19201 agtccctttt taaataaatt gcgcgtttgg ttacggtttg tgcgttttgc tgcttattcc
    19261 tccagtttat gcgctttccc gtggcgatta aggccaaaaa ctttataaat cgccgtcacc
    19321 gccagcataa agatgatgcc aacaaacagc gacatacgcg tatcttcatt aaagtacatg
    19381 ccgatcaaaa cgcaaatcag gaatgccatt gttacgtagt tggcccacgg gaacaggatt
    19441 gagcggaacg gatggctggc aatcgccgct ttatgtgcac gccgaaaacg cagctggctt
    19501 atcaatatca caaaccatgg caccatcccc ggaagcacgc tggcactgta gacgtagaca
    19561 aacacacgct gcggattggg aatgatgtag ttcaggcatg agccaattag cagaatagca
    19621 atagatactg ccacacccgc aactggtacg ccgtgacggg aaactttcgc catcgccgcc
    19681 ggtaactgac ggtttttcgc cagtgcgtag agcatacgtc cgcaactgta catgccgctg
    19741 ttacagccag agagcgcagc tgtcagcacc acaaagttga taatgcccgc cgctgcggta
    19801 ataccgattt tggcaaaagt cagtacgaac gggctgccgt tgctgccaat ttcattccac
    19861 gggaagatgg tgacgataac gaaaatcgcg cctacgtaga aaatcaggat ccgccacagc
    19921 actttgccta cggcactgcg cagcgtcacc tgcggattct tcgcttcacc ggcagtaatg
    19981 ccaatcagct ccacgccctg gtaggacgcc accacaatac acagcgcggt caggaaccct
    20041 ttccagccac ccgcaaagaa accgccatgc tctgtgagat tgctaaaacc aatcgactgc
    20101 ccgccattgc caaagccaaa gaaaatcacg cccaggccaa tgacaatcat cacgataatc
    20161 gtggtgactt tgatcatcgc gaaccagaac tcgatttcgc cgtacaaccg caccgccgcc
    20221 agattcgcca acgccaccag cgccactgcg atcaatgcgg gtatccactg cgccatctcc
    20281 gggaaccaga actggacata aacgccaatg gcggtgattt ctgagatccc cacggccatc
    20341 cacataaacc agtaagacca ggcggtgaga taaccaaaga acgggctcat ataacgatgc
    20401 gcataaacgg cgaacgaacc ggtaaccggt tcgaggaaca acatttcgcc cattgaacgc
    20461 atgatgaaaa agacgaatag cccggcgatg atataggcca acaatacgga tggcccggcc
    20521 catttcaagg tactggcggc ccccataaac aggccgacgc caatggtgcc ccccagggcg
    20581 atgagttcga tatgtcgagc ttccagccca cgctgtagct ctggtttgtt atctgccata
    20641 aatcctcgtg ttgtgtttgc atgctttccg gtgttaccgg ttatcgttat gggtacatcg
    20701 agtgttgcga atgttttcgt aattcaggag aaatggcaaa taaagcatta aaaatttgaa
    20761 tgctttgtgt aataaaaaag cagacaggcg acggagtgac cactccgtcg ctttacaaag
    20821 agaggaaaat cagaggttgc cggtgtagtg ccagcgtaaa taacgcagca aacgaagctg
    20881 acgcttaatg cggctcggct gcgaaagcag gcggtagagc cactccagtc ccagcgtttg
    20941 ccagattttc ggtgcgcgtt ttacgtgacc ggtgaaaaca tcgtaagtcc cgccaacgcc
    21001 catatacagc gcatctggat gcaccagacg gcagtcgcgc atgaagatct cctgctttgg
    21061 cgatcccatc gcaacggtga cgatttgcgc tccgctggca tgaatgcgtt caaacagcgc
    21121 ctgacgctgc tcgggtttaa aataaccatc ctgactgcca acgatattca cattccattg
    21181 gttgcgcagt ttagcttcag tttgcgccag cacttcaggt ttaccgccca caagaaatac
    21241 cggcgtccct tctttgcctg cgcgcgccat cagctcttcc cagagatcgg caccggcaac
    21301 gcgggaaacc tgcgcctgcg ggtacttttt acgtactgaa cgtacaacgc tgatgccatc
    21361 cgcatattta aattcggcag cgttaattaa ctccctgacc tcggcgttat cttcaatggt
    21421 cagcattttt tcggcattaa tggcaaccag cgttccctgc ttaagctgcc cgtcagcaaa
    21481 cagataatcg agggcgtgct gcatatcacg ccaaccaatc aactgtaagc cacgcagcgt
    21541 ataggtcggt gccgtggtgt tgttattcat tgttatcctt caacctgcgt ccggagcgat
    21601 gattttgtac gtttatgaat gagtccggcg ctttcaaaaa gccagtacaa cagttttgcg
    21661 atcatcagac atgcgccgaa gaccacgata aaaaagacta cgcgtgagac aaacgaatcc
    21721 agcccttcac gtgccagcac gatcatattg aagatcgccc caaagcagaa actgtgcaat
    21781 atcgcagcct tatagcgatt aggctcgcgg ttgcccagct catacagcca gtcgaaccat
    21841 ttgatgatca gaccaaccac gatcgccccg agcgggatga acaacgcgcc gcccatcacc
    21901 accagtgagc ctataagcgt aggcgagatc gccagtccgg agtggttatt cagcacttcc
    21961 caggtaaagt agttggctga gttcagcacc atactcgggc gacccggcca cagccaggaa
    22021 gggataaaga catagaaatc gcggacaatt ggagccaggc cctggaagtc gatgttgtcg
    22081 tagttctgca acagcaacgc cagattctcc cacggcgaga aggtgtcgcg agtgagatag
    22141 agaaacgtat agaacgcttc atcgccgctc acattcattc catagcgttt tagcgccagc
    22201 cagaacatgc ccacaatccc cagcacgccc gccgccgcca gcatccacaa cgaaatccag
    22261 ccgcgaataa tgccaataaa caggaagata gcgaatgcga tgatgatatt ggcgcgagtg
    22321 ccgccgacaa tcatataagt cagcaagcca aaggcgaccg tgctgacgag gaaaaacagc
    22381 cacgctttgc tatcctggcg cagaaagtag accactagca tcgccgggat gaaaaagtaa
    22441 aagaagcgtt ttaacgccac gccggagact tcactggaga agatctgact gtaggagtta
    22501 agccggaaca gcaaaaagcc gttgtgcatg aagaagatgc cgacgcttac cagcgcgatg
    22561 cccatcagga tcacccacgt aagattggtc tccacgcggt tcatggtaaa cagcggacgg
    22621 cgcggtacat cagcaacgcg tttgcgtagg cgggttttgt aggtgacata gtaaaccgcg
    22681 tagaagcagc ccgcagaaag caacgcctgc aacaagattt ctggcggcgc gacaccaaca
    22741 tcaaagcgaa ataccagcac gctggtcagc gggaagccga agaaaaaggt gagcaaaaac
    22801 agcaatgaaa agaagacatt gaagttaaag cgcacacggc gaaactcaaa ccaggtcagc
    22861 gtggcaataa acagcgtgca gagcagccag accacaaaca ggccactgaa ttgcagcaga
    22921 ctcatgcgcc ctccccggcg gcaatcgcca acgcccgctg ccagccttgt agatagttag
    22981 ggctaaagaa ggcaatggtg tttttatcca ccgacgccaa ctggcgctgc gcttcacgca
    23041 caatatcctc gttgagatcg tcggtagtaa acagcactgg caaatgctgt tccgtcatat
    23101 cctgccagaa cggattttcc cggttaagca cacaaggaat gcccgcctga atcagtaagc
    23161 acagcgtacc aatgccctgc tggcgagcaa aaataaagta accaagatcg cactgacgaa
    23221 gtagcgccag ataggcgtca aattccagtt tttcgctcaa aatttgcaga ttttcttcgc
    23281 tgaataactc cagccccgcc tgacgaactt cctcaatgta cgcttcgtta ttaggcggat
    23341 atcccatcgg caccaccact tttaccgtat cgccaaattg ctgatgaacg gcacgtaaag
    23401 cggcgacatg ctcattgctg aggtcgccgg agttccccac cagaatggtc atttttcctt
    23461 cacgttgccg atcgttcgcc atcgtattga gagaagggtc catccgcgtc gggaagtaca
    23521 gcagttctcc ccgcaccttt gggtgcgttt tggcaaaaaa gctcaaatcg ccgcgggtgg
    23581 caaatacaca gccgactcgc ttttgcgcca ggcgacgtag tgggtaaaaa agcttatatc
    23641 tcaagccact ggaaagctca tacaggtctg ccccccagat atgccagaaa aactggctgg
    23701 gcttaatgcc accactcagc agagccagcc acagtgtggg attgaactga ccgtggaaaa
    23761 aaaatcgctg ctgacggtta gcttttgctt tcgcgatgac cgcttccgcc agcgattttt
    23821 tcccagggaa aaattgcaca gaaagcgccg gacagctatc acttaagccg tcgtccttgc
    23881 caacaaccat aaactcgcgc gcgtgctcgc tcgtcgcagc cagcgcgtca ttgaaaaacc
    23941 gcaaaacggt tcggttatgg tgagggatat ccgatcccag tacgtgaatc agtacagtca
    24001 tgcccgccta cgccagagta aaaacacgcc acaacaaaga gaaaaataga cgatataagt
    24061 tgccatatat gcctgcgccg cgcccagtgc accatgcgca gggattagcc agtgggcaaa
    24121 taccatcaat aaagtgaact ggctgatttc cgccagaata taaaaccgca atgacgcttt
    24181 ggcgatcacc agataaccaa agacataagc gcccactttt aacacatcac ccactaactg
    24241 ccaggcaaag agatcgcgca tagcggtaaa tttattcgac aacagcagcc agatagcaaa
    24301 atcacgcagc agccagacgg taaaactcgc cgccgccact gccggtaaga cgaatttcag
    24361 cgatttaacc acttcccggg tgatatcgcg cttttccgtt agccgcgaca acgtgggcag
    24421 caaatatacg ctgaacgatg ccgtaataaa ttgcaggtag gcatcggaaa tactgctcac
    24481 cccttgccag atccccactt catcccagct atactgcgcc gccagcagtt tacgcatcat
    24541 gatgtaagca acaggcaagg tcaccgacgt aatcaacgcc atgagcgtaa atttgctcaa
    24601 ctgccctgcc agaccgttat cccagctggg tttcagatag cttaacggga tgacaccacg
    24661 tttgatcaac atgatagcgg caggaattac caccagcgcg ggaatcagcg ccagacccag
    24721 caacgcccct tcataaccgc ccaaacggta actgacgtag tacgcgagaa cgccaatcaa
    24781 gctgccgaca atcagcgata acgcattacc tgcggcatcg cgaaagcctt tcatcagcgc
    24841 cagtaacagg ttgccccagg cgatccccat ttgcaccagc gccactaaac gcaccagccc
    24901 ctgatagtcg gtataaccaa acaatccctg gctgattggc gcagctgcca gcacaaaaac
    24961 cagcgccatc agcgtagaga aaccaagtac catcgctgat gaagtgccga ccacgcggcg
    25021 cagctgttgc ggattatcat ggtactgggc aacgtatttg gttacaccgt taaagatgcc
    25081 agctccggca agcacgccga gcacggtaat caactggcgg aaatttgccg ccagcccaag
    25141 ccccgccgga ccaaatgaca cagccagcaa cttaccgacc agtaacccgg caccaatctt
    25201 gaccagtgta ctggccgccg tccacaagga cgcttttgcc aacgacatat caggagaagt
    25261 agttcaacaa agtcgcaatt accgtacgct gattgacggg cgacaggttg tagaacagcg
    25321 gcaggcgcag caggcgctcg ctctctttgg tggtgtagcg atcttcaccg tggaactcac
    25381 caaagcgttc ccccgcaggg caaccgtgca gcggaatgta atggaacacc gccatgattt
    25441 ccgcttcttt cagaaagtta atcaacgcgc tccggtcatc aatatcccgc agtttaatgt
    25501 agaacatatg cgcgttctgc acgcagccat cgggaatcga cggcagctcg atacgcccgg
    25561 ctttcgccag aggcgctaac gcatcgtagt agttttgcca cagcgccaga cgttgctggt
    25621 tgatacgatc cgctgcttcc agttgcgccc acaggtacgc agcttgcaga tcggacatca
    25681 aatagctgga gccaatatcg cgccaggtat atttatcgac ctgaccacgg aagaactggc
    25741 tgcggttagt gcccttttca cggatgatct cggctcgttc gattaacgct ttatcgttaa
    25801 tcagcgtcgc gccgccttca ccacccgccg tatagttttt ggtttcatgg aagctaaagc
    25861 agccaatatg accaatggtt cccagtgcac gccctttgta agtggacatc acgccctgag
    25921 cggcatcttc taccacaaac agattatgct ttttcgccaa cgccataatg gtgtccattt
    25981 cgcaggccac acccgcgtaa tggaccggca cgataacgcg cgttttgtcg gtgatcgccg
    26041 cttcaatcag cgtttcgtcg atgttcatgg tgtccgggcg aacatccaca aaaacgattt
    26101 ttgcgccacg cagcacaaag gcattggcgg tggagacaaa ggtgtagctc ggcatgatca
    26161 cttcatcgcc aggctggata tcgagcagca gcgccgccat ctccagcgaa gcggtgcagg
    26221 acggcgtcag taacactttg gcgctgccaa aacgttgctc cagccactgc tggcagcgac
    26281 gggtaaaacc gccatcgcca cacagtttgc cgctacccat tgccgactgc atatagtcga
    26341 gttcggttcc caccaccggc ggtgcgttaa atggaatcat gtgatcacct gtataaccag
    26401 tacgcggtgc tttctacatt cgcaccactt tgtatgtatc gtttaagcgc ggcggtgttg
    26461 cccatttggg tcgccacccg caaagttgtt ttaccgcgag catacgccca gtttagcgcc
    26521 gtttgcatca gctcagcacc tgcaccgcgt ccagccagca ggccaattcg cgcatctgtc
    26581 gcattgagtt cccgtaaaga gacataaccg cgaataccgc cggacgccgc acgtaaaatc
    26641 agacattgat gatcaaaggt gccgcgcacg gcattttcaa tccactgtgc ataaaagcgt
    26701 ccgctggcgt caggcgcata ccacggcgca cgaaaacggc tttgcgcaaa tgcggcgctg
    26761 gctaactgac gtaatgcggg aatatcggtc tcttgtgcca ctacagcacc actatcactg
    26821 acattgttca cgggtagtgc caaatcaact tcaccttcta ccagggagaa tcccagctgt
    26881 tgcagggcat ccagttcacc cgtatttgat gccgcaattt tggcctgcac ccgtgaccac
    26941 ggcgctaaca cgtctggcgt caggagcggt gcttcagacg taatgcgcac gatggcgctg
    27001 ttaacaccaa agaaggcgtt ttcccaggtt agtggctcaa tactggcgcg gacgggcacg
    27061 aagtaactcc agcagatatt ggccgtagcc agttttcgct aatgaactgg cagcacgctt
    27121 cacaccctcg tcatcgagcc agccgttacg ccaggcgatc tcttccaggc aggcaatctt
    27181 aaagccctgg cgtttttcta ctgtctgcac aaaggtactg gcttcaatca ggctgtcgtg
    27241 agtgccagtg tccagccagg caaatccgcg cccgagcagt tcaacggtca ggttgcccgc
    27301 ctcgaggtac atctggttga tggaggtaat ctccagttca ccacgctccg acggcttcac
    27361 ctgctttgcg tactccacga ctttactgtc gtagaaataa agcccggtca ccgcccagtt
    27421 tgacttcggc tgttttggct tttcttccag cgagatagcg cggaaattgt cgtcaaactc
    27481 caccacgcca aagcgttccg ggtccatcac ctgatagcca aataccgtcg ccccttccgt
    27541 gcgcgccgca acatgacgca gcttcggact gaagccctga ccgaagaaga tgttatcgcc
    27601 cagcaccaga caagaaggtt caccgttgag gaaggtttca ccgatgataa aggcctgcgc
    27661 cagaccgtcc gggctgggct gttcggcgta ttccagctgg ataccgaact cactaccatc
    27721 gcccagcagg cgctggaaat aacctttatc ttccggcgta gtgatgatga gaatttcgcg
    27781 gataccggcc aacatcagca ccgacagcgg atagtaaatc attggcttat cgtaaatcgg
    27841 caacagttgc ttcgatacgc cgcgcgtaat cggatgcaat cgggtgccgg aaccgcccgc
    27901 caggataata cctttcatgt ttgcctccgc tgaaaattag cctttcagac ctaaacgctc
    27961 gccctgatag ctgccgtcct gcacctgctt ccaccagctt tcattagcca gataccactg
    28021 caccgtttta cgcattccac tttcaaaggt ttcctgtggc agccaaccaa gttcacgggc
    28081 aattttcgaa gcatcaatgg cataacgcag atcatgcccc ggacggtcag cgacaaaggt
    28141 gatcaagtca cgatagtgcg ccacgccgtg cggcttgttc ggagccagtt cttccagcag
    28201 ctcgcaaatg gtttccacaa catcgagatt cttacgctcg ttgtgaccac caatattata
    28261 ggtttcaccg actttcccgg tggtcgccac gcaatacagc gcgcgggcgt gatcttccac
    28321 atacagccag tcacggattt gctgcccgtt gccatatacc ggcagcggtt tacccgccag
    28381 cgcgttgagg atcatcagcg ggatcagttt ttccggaaag tggtaaggac cgtagttatt
    28441 cgagcagttg gtgatcagcg tcggcagacc gtaggtccgc agccaggcgc gcaccagatg
    28501 gtcgctactg gcttttgacg cggaataagg gctgctcggc gcatacggcg tggtttcggt
    28561 gaagaaatca tccgtcgagt gcaggtcacc atatacttcg tcggtggaga tatgatgaaa
    28621 acggaacgct gattttttat cttccgtcag cgtattccag taagcccgcg ccgcttcaag
    28681 caatgtataa gtcccgacaa tgttggtttc aataaacgct gccgggccgt caatagaacg
    28741 gtcaacatgg ctttcggctg ccagatgcat gacacagtct ggctgatgct cagtgaatac
    28801 gcgtgccagt tctgcccgat cgcagatatc aactttctca aaggcaaagt gctcgctttg
    28861 cgcgaccggt gccagcgaca tcaggtttcc ggcgtaggtc agcttatcga ccactaccac
    28921 cgcatcgctc gtttcgttga tgatataacg caccagcgcc gagccaataa acccggcacc
    28981 acctgttatc agaatttttc tcatcagcgc cagactcctt tggcatcgac gacatactgc
    29041 tgatggacat tgtcgccatt gataactttg aactgactat gatcgaccag catcaccagc
    29101 acgtctgccg ttgccagcgc ctcgtcaagc tgcgccagag tacaaagccc ggtcagtttt
    29161 ttcggcaact ggtggatgtt aggctcaaca accagcgttt cgccgctgtg ccactgggcg
    29221 atcagttcag cgatttccat cgccgggctt tcgcgcaggt catcaatatt cggtttaaac
    29281 gccagaccaa agcaggcgat tttcagttca ctggcgcgtt tatcggtagc agcaaggcag
    29341 tcggcaaccg ccgctttcac ctgatcgata acccagaacg gtttgtgatc gttaacttcg
    29401 cgcgcggtac ggataagccg cgcctgctgg gggttctgtg ccacgataaa ccacggatca
    29461 acagcaatgc agtgaccgcc cacgccaggg ccaggctgaa gaatattaac gcgagggtga
    29521 cgattcgcca ggcgaatcag ttcccagacg ttaatcccct gatcggcaca aatcagcgac
    29581 aattcattag caaaagcgat attcacatcg cggaagctgt tttcggtgag cttacacatt
    29641 tccgccgtcc gcgagttagt gacgacacac tcaccttcga ggaaaatttt gtacagttcg
    29701 ctggcgcggg tcgaacaaac cggcgtcata ccaccaatca cgcgatcgtt tttaatcagc
    29761 tcgaccataa cctggcccgg taacacgcgt tccgggcagt aagcaatgtt gacgtccgcc
    29821 tgctcgccca cctgctgtgg gaaagtgaga tccggacgca tctctgctaa ccattctgcc
    29881 atcttctcgg ttgaccccac cggcgaggtg gattcaagga tcaccagcgc gccttttttc
    29941 agcactggcg caatggagcg agcagccgat tcaacgtagg tcatatctgg ctcatgatcg
    30001 cctttaaacg gcgtgggtac agcaatcagc caggcatccg cttcaactgg cgtcgtgctc
    30061 gctcgtaaaa aaccgccttc tacggcagtt tttactacgc tcgccaaatc aggctcgacg
    30121 atatggattt cgccacgatt gatggtatca accgcgtgtt ggttgatatc gacaccaatc
    30181 acctgttttt gccgtgaggc aaacgctgct gctgtaggca gcccgatgta acccagtccg
    30241 ataacagaaa tggtcgcaaa actcatagtg atatccgatt attttttaac gcttccagaa
    30301 tgcgagagca tgcctgacca tcaccatacg ggttatgggc gcggctcata gtttgatatt
    30361 cgttttcgtc ttttaaaaga cgcgtcactt cctcgacaat tcgctgctta tccgtgccta
    30421 ccagacgcac cgtacccgcc gtcaccgctt ccggacgctc agtggtatcg cgcatcacca
    30481 gcaccggttt ccccagcgaa ggcgcttctt cctgaatgcc gcctgagtcg gtcaaaatca
    30541 gccaggcgtg gttcatcagc cagacaaacg gtaaatactc ctggggatcg atcagaatga
    30601 catttttcac atgccccaga atgcgattga ccggctctct gacgttcggg ttgagatgca
    30661 ccggatagac aatctggatg tcctggtgcg tggtggcgat gtctgccagc gcgtggcaga
    30721 tttcttcaaa gccacgaccg aaactttcac gcctgtgacc ggtcaccaga atcatctttt
    30781 tatcggggtc gataaacggg taatttgccg ccagttctga acgcagcgtg tcgctgctca
    30841 tcacctgatc acgcacccat aacagtgcat caatgactgt attaccagta atgaagattc
    30901 ggctatccgc aacgttttca cgcagcaagt tttgccggga agtttcggtt ggagagaagt
    30961 gatacatcgc cagatgcccg gtcaatgtac ggttagcctc ttccggccac ggcgaataga
    31021 gatcgcccgt gcgtaaacca gcctcaacgt gaccaacagg aatacgctga taaaacgccg
    31081 ccaggctggt tgccagcgtc gttgttgtgt cgccgtgaac cagcacgacg tctggtttga
    31141 actcggcaag aataggtttt agcccttcca gaatccgaca ggttatctct gtcaggccct
    31201 gtcctggctg cattatgttg agatcgtagt caggtacaat ggaaaagagt ttcagcacct
    31261 gatcgagcat ctcccgatgc tgcgcagtga cgcaaacttt agcctcaaaa aaaggatctt
    31321 ttgccaacgc atgcaccaac ggagccatct tgatggcttc cgggcgcgta ccaaatacag
    31381 tcagtacttt cacatcgatt ctcttcgaat aagcggcgag cgcctttgcg ctcgcagcag
    31441 cagtgttgct atttcgagca acggcgggtt aatgcgacac cagccccgat cagccccccg
    31501 acaatgcccc acataatcat caggaaggca cgacgtgggc tatcgcgttt taccggttct
    31561 tccggcgtac gcaaatagcg ataggtctga aaacgcggat ccagagttgg accaacattc
    31621 agggtgttta acatggcccg attctgatca tagtcgagat caaaggccgg accgacggcc
    31681 tgtaaatttt ccagtcgagc ctggagcatt ggacgcccaa gcaggaacat ttctgaatca
    31741 ggtaattcct cggcaggcac atctgtcgca ctgcgcgaaa tattatgctg ctcagcaatt
    31801 ttcagcgcct gctcaatgct gttcatccgg cggtcgtaga tggctttcgc cacctcttcc
    31861 tgacgcttca cctgagcttt catctggatg gtgcgtgccg cccatgcgcc tttcagctca
    31921 tcattcagat ggctggctgc acgctggctg gcaaaagcaa catactgacg taacaggtta
    31981 ttagcgtcag gcgcggtttc ggcaataagc ttcacgctgt cattgaccgc gcgggtaaag
    32041 tctccgggga taaactggat gttgttaatc atttcatcca gcaacgccgc atcggctttg
    32101 ctgttgccca ccatccgctg tttgtaatag tcggtttgca gccagaactc tctgcgggta
    32161 tcccacgagg ccagttgcat aacaaactct ttataggctt cgtccatgac cgatggttga
    32221 tcggcagaag ccatgtttga acggacatcc aggttacgca aaaattgctg ctgcgagtaa
    32281 tatcccccca gcatattcac cgttggacga tcggtaatcg ccgtcgagct ccactcctga
    32341 cgagcaaaaa aagtatacgc cagcgcgatt aacgcaaacg ccagccccat gccaataatc
    32401 catagcttcc cagcccacaa ggtacgaaac aacccacgaa tatccagttc attttcagcg
    32461 tcttcggccg gtttcccagg cattggttgt gtcatcacat cctcatttat ttggttaaat
    32521 tggggctgcc accacgattt ctacgcagtc tgcgttttac gcgcttaata aagcgagcaa
    32581 ctttccaggc acgcttaatg caatatccat agaggaagaa tgctagcaaa aagagcacca
    32641 gcatgaccca ctccgggaca aaatgagaat attctgccag cacgccaatg gaagcgagca
    32701 gtgctgcggc aagggtaatc agcacaaacg cctgacggga agtaaacccg gcacgcatga
    32761 tcaaatggtg aatatgctga cggtcaggag agaatgggct catgccttta cgcaggcgac
    32821 ggtacataat cgccaccata tccattagcg gaatggcgat tatccacaaa gcggtaaccg
    32881 ggctgatggg atgggttttg ccctgggtcg tttcgagcag gatccagata acggtaaaac
    32941 caatcagcgt actgcccgca tcacccataa agactttgta gcggcgaccc aggataccaa
    33001 ggttaagcat gatgtatggc aggatggcgg cgatcatcgc aaagcaccag attgcgaggc
    33061 tggtttgccc gtcgaaccac aaaatcatac cgattgctgc aaacgagacg caggacaacc
    33121 cgcccagcaa gccatcaatg ccatcaacca tgttgaacgc attaatggcc gcccagacgg
    33181 caaatagcgt caggaagtaa ccaaacggtc cgagcaccat ctcccaggag ccaaagatat
    33241 aacccaggct actgagataa agtttgccga acaccatcat aacaatgcca acagcggcct
    33301 gtatggtggc acggattttt acgctgatat caaaacggtc atccagcgcg ccaatgaaaa
    33361 caagcacacc ggcacaagcg agatagagag atgcatgcgg aatatagtaa tcgacaattc
    33421 cgaacgtgaa gcaaatccct gcgtaaaccg aaatcccccc aacgagaggt atcaatccct
    33481 ggtgacgttt gcggaagttt ggtttatcca ctaaaccgac tttttttgcc actttacggg
    33541 caaaaaacag aaacagtgtc gtgaataaaa aaatactgat gagatcagta ctcactgtca
    33601 gtaaattcac aatgcatgct ctcagagaaa attattagca gaagtataac cacgaagacc
    33661 tttattcaga agggaaagtc cgttccgaac ccaccagtcc tgtgcaaaaa ttaaccattt
    33721 gatatgcaaa ttgcataaca tgccagcaaa ttccaggaat tttcctaaca tcgccagcgc
    33781 ggcattaaga ttacagatat aaaagcaaaa cgccacgtaa acacgtggcg tttttggcat
    33841 aagacaaatt tatgagcgtt tcatcatttc gaagaaatcg tcattggtct tagtcatggc
    33901 cagtttatta atgaggaatt ccattgcatc gatttcgccc atcgggtgaa tgattttgcg
    33961 caggatccac attttctgca gttcttcctg agtcgtgagc agctcttctt tacgggtacc
    34021 ggaacggttg tagtcgatag ccgggaagac gcgtttttca gcgatcttac gagagaggtg
    34081 cagttccatg ttgcctgtac ctttaaactc ttcgtaaata acttcgtcca ttttagaacc
    34141 ggtatcgata agcgccgtcg cgataatggt caggctgccg ccctcttcca cgttacgcgc
    34201 cgcaccgaag aagcgtttcg gacgatgaag ggcattggcg tccacaccac cggtcaacac
    34261 tttacctgac gccggaacaa cagtgttgta agcgcgcgcc agacgagtga tggagtcgag
    34321 cagaatgata acgtctttct tgtgctcaac caggcgtttc gccttctcga tcaccatttc
    34381 cgcaacctga acgtggcgag atgcgggttc gtcaaaggta gaagcaacaa cttcaccttt
    34441 taccagacgc tgcatctcgg ttacttcttc cggacgttcg tcgatcagca gaaccatcag
    34501 cacacaatcc gggtggttgt aagcaatgct ctgagcaatg ttctgcagca gcatggtttt
    34561 accggctttc ggcggtgcca caatcagacc acgctgacca cgaccgatag gtgatgccag
    34621 atccagtacg cgagcggtta aatcttcagt agaaccgtta ccacgttcca tacgcagacg
    34681 agagtttgcg tgcagcgggg ttaagttctc aaagaggatt ttgttgcggg cgttttcagg
    34741 tttgtcgaag ttaacttcgt taactttcag cagcgcaaaa tagcgttcac cttctttcgg
    34801 cgggcgaatc ttaccagaga tggtatcacc agtgcggagg ttgaaacggc ggatttggct
    34861 aggggaaacg tagatgtcat caggaccggc gaggtaggag ctgtctgcgg aacggaggaa
    34921 accaaatcca tcctgcaata tctccagtac gccatcacca aagatatctt cgccactctt
    34981 tgcgtgctgc ttcaggatgg caaaaataat gtcctgctta cgcatacgag ccaggttttc
    35041 cagccccata ttttcgccga gagtgatcag ctcagaaacc ggcgtattct ttaattcggt
    35101 aagattcata gtggtgtgag ttcttaaact tggggtaaat ctcgaactta atgttgtgaa
    35161 tggtatggca ggatcatcca tgcctgttta cggtcatcga ctcatgtctt ttcgctgcct
    35221 ggtcacagga gagtacgcag aactgaaacg acaagacgga ttgagtgacg agccagaaat
    35281 ctgttcactt cgcatttaag ataaaacggg aagcgttgga aattacaaga ttcaaactta
    35341 ataaggtatg tttaatacga agtcaacact aagttagcat gactcacgcc gggcgtccag
    35401 tttttagcga cggggcaccc gaacatgaaa ttcccttacg ccaggttagc gtcgaggaac
    35461 tctttcaact gacctttaga cagtgcgccc actttggttg ccgccacttc accgtttttg
    35521 aacagcagca gagtcgggat accacggatg ccatatttcg gcgcagtgcc agggttttga
    35581 tcgatgttca gttttgcaac ggtcagtttg ccctgatatt cgtcagcgat ttcatccaga
    35641 atcggggcga tcattttgca cggaccgcac cactctgccc agaaatcgac gaggatcgcc
    35701 ccgtccgctt tgagtacatc cgtgtcaaaa ctgtcgtcag tcaggtgaat aattttatcg
    35761 ctcatatata actccacagg aataagcctg gcgtgttggt ttcgttgttg gtgtaacatt
    35821 aaccaactaa aggttgactt tatttcaccg gatacgcttt cgtaaagcaa tagtaagctg
    35881 atattctacc acactatgag caaaacacat ttaacagaac agaagttttc cgacttcgcc
    35941 ctgcatccga aggttgtaga agcccttgaa aaaaaagggt ttcataactg tacgcccatt
    36001 caggcactgg cccttccgct gacgctggcg ggtcgtgacg tagccgggca ggcgcaaacc
    36061 ggtaccggga aaacgatggc gtttcttacg tcaacgtttc attatcttct ctctcatcct
    36121 gcgattgccg atcgcaaggt gaatcaacca cgtgccttaa ttatggcacc gacgcgtgaa
    36181 cttgccgtgc agatccatgc cgacgcagaa ccgctggcgg aagctaccgg cctgaagctg
    36241 ggtctggctt acggtggtga tggctacgac aaacagctga aagtgctgga aagcggcgtt
    36301 gacattctga ttggcactac ggggcgttta attgactacg ccaagcagaa ccacattaac
    36361 ctcggtgcca ttcaggtggt ggtactggac gaagccgatc gcatgtacga tctgggcttt
    36421 attaaagata tccgctggct gttccgccgt atgccgcctg caaaccagcg cctcaacatg
    36481 ctgttctccg ccacgctttc gtaccgggta cgtgaactgg cgttcgagca gatgaacaat
    36541 gccgaatata ttgaagtgga accggaacag aaaacgggcc accgtattaa agaagagctt
    36601 ttctaccctt ctaacgaaga aaaaatgcgt ttgctgcaaa cgctgatcga agaagagtgg
    36661 ccagacagag cgattatttt cgccaatacc aaacaccgtt gtgaagagat ctggggccac
    36721 ctggcggcag atggtcatcg tgtcggtttg ttgacaggcg atgtcgcgca gaaaaaacgt
    36781 ctgcgtattc ttgatgaatt tacccgtggc gatctggata ttctggttgc caccgacgtt
    36841 gccgcgcgtg gtttgcatat tccggcagtg acgcacgtct ttaactacga tttacccgat
    36901 gactgtgaag attacgttca ccgtattggt cgtacaggtc gcgcaggcgc aagcggtcac
    36961 tctatcagcc tggcgtgtga agagtatgca ttgaatttgc ctgctattga gacctatatt
    37021 ggtcactcaa ttccggtaag caaatacaat ccggacgcat tgatgaccga tctgccaaaa
    37081 ccgctgcgcc tcacgcgccc acgtacaggc aatggcccgc gtcgtactgg cgctccgcgt
    37141 aatcgtcgtc gttcaggtta aataaaatgt catactcaaa atcattcaaa ctgtatcaag
    37201 atggcaagag aatgaatccc gatgagcata ctgtagtaag tgattcaggt gaaagaatgc
    37261 agccaacaca gagacagatt gaaggatgaa gagtatgggt tccacctcgt cgctgtatgc
    37321 agccattgat ctcggttcga atagttttca tatgctggtt gtgcgcgagg tggctggaag
    37381 catccagacg ctgacgcgaa taaaacgcaa agtgcgtctg gctgctggcc tgaatagcga
    37441 aaatgccctg tctaatgaag caatggagcg cggttggcaa tgtctgcgcc tgtttgctga
    37501 acgtctgcaa gatatccctc cctcgcaaat tcgcgttgtc gctacggcga cattacgtct
    37561 tgccgtcaat gcgggtgatt ttattgccaa agcgcaggaa atcctcggtt gtccggtaca
    37621 ggtgatcagc ggtgaagagg aagcacgtct gatttatcag ggcgttgctc acacaactgg
    37681 cggtgccgat cagcgcctgg tggtggatat aggcggtgcc agtactgaac tggtaaccgg
    37741 cacgggtgca caaaccacct cgttgttcag cctgtcgatg ggctgcgtca cctggctgga
    37801 acgctatttt gccgatcgta atctggggca ggaaaatttt gatgctgcag aaaaagcggc
    37861 acgcgaagtg ttacgtccgg ttgccgatga attacggtat cacggctgga aagtgtgcgt
    37921 tggtgcttcc ggcaccgtgc aggcgttaca ggaaatcatg atggcacagg ggatggatga
    37981 acgcattacc ctggaaaagt tgcagcaatt gaaacagcga gccattcatt gcggtcggct
    38041 ggaggaactg gagattgacg ggctgacgct ggaacgtgcg ttagtgttcc cgagtggtct
    38101 ggcgatcctg atcgccattt ttaccgaact gaatattcag tgtatgaccc tggcgggcgg
    38161 tgcgctgcgt gaaggcctgg tctacggcat gttgcatctg gccgtcgagc aggatattcg
    38221 cagccgtacg ctgcgtaata ttcagcgccg ctttatgatc gatattgatc aggcgcagcg
    38281 cgtagccaaa gttgcggcta acttcttcga tcaggtggaa aaggaatggc atcttgaagc
    38341 aataagccgc gatttgctca tcagcgcctg tcagcttcat gaaatcggcc tgagcgttga
    38401 ctttaaacaa gcgccgcaac acgctgctta tctggtgcgt aatctggatc ttcccggttt
    38461 tacccccgca cagaaaaaat tgctggcgac gctactgctc aaccagacta atccggtcga
    38521 tctctcatcg ctgcatcagc aaaatgccgt acctccgcgc gtcgcagaac aactctgccg
    38581 tttactccgc ctggccatca tttttgccag ccgtcgccgt gacgatctcg tgccagagat
    38641 gacattacag gctaaccatg aactgttgac cttgacgctt ccgcaaggtt ggctaaccca
    38701 acatccgctg ggtaaagaga ttattgctca ggaaagccag tggcagagct atgtccactg
    38761 gccgctggaa gtgcattaat gagtaagtgc cggatgcgat gctgacgcat cttttccggc
    38821 cttgattatt tccctcgttt tgccgccatc atcgctttca gattcgccag atggctttgc
    38881 cctttctgca tccgttcttc ggcgctgacc actttgcgct cctgttccca aatcagatca
    38941 tcctgcggca gctccagcaa aaagcggctc ggctccgggc gcaccagttc gccgtactga
    39001 cggcgttctt tacacagcgt aaaggtcaat tccttctggg cgcgggtaat gccgacatag
    39061 gccagccgcc gctcctcatc gatattatct tcatcgatac tgctctggtg cggcaaaaac
    39121 ccttcttcca taccgaccat gtagacataa ggaaactcca gccctttcga cgcgtggaga
    39181 gtcatcagtt gcacctgatc cagctcttct tcactctcac cacgctccat catgtcgcgc
    39241 aaagtaaagc gcgtcaccac ctgggtgagc gtcatcggct catccagttc gctaccttcc
    39301 agcatctccg tcatccagct aaacagttgg ttgacgttct tcatgcgcat ttcggcggct
    39361 ttcgggctgg gcgatgtttc gtacagccag gattcataat ccatgccgtg gatcagatcg
    39421 cgcaccgcgg caatcggctc ccgctccgcc agacgctgga tttctgccaa ccagtgagta
    39481 aagcgggtca atgcttcata accacgtccg ctaagcgtct gactcaggcc catatcaaag
    39541 ctggcggtaa acatgctttt attgcgcgtc atcgcccact cacccagctt tttcagcgtc
    39601 gccgggccaa tctctctctt cggcgtgtta acgatacgca gaaatgcgct gtcatcgtcc
    39661 gggttagtca gcacgcgcag ataagccagc aagtccttga tttcaggacg agagaaaaac
    39721 gacgtaccac cagatatttt gtatgggatg cggttttgca tcaggaattt ttcaaacacc
    39781 cgcgactgat ggttaccgcg ataaagaatg gcgtaatctt tgtactgcgt tttattgacg
    39841 aagtgatggg cgatcagctc gccagtaacg cgctcggctt catgttcctc gttattcgcg
    39901 cttaaaactt ttagctctgt gccgtaaccc agttcggaga acagacgctt ttcaaagacg
    39961 tgcggattat tggcgatcag gatgttcgcc gctttcaaaa tacgtccgga agagcggtag
    40021 ttctgctcaa gcttaatcac cttcagcgcc ggaaaatcct ggctcagcag caccaggttt
    40081 tgcggacgtg cgccgcgcca ggagtagatc gactggtcat cgtcacccac cacggtaaag
    40141 cgcgcgcggc tgcccaccag cagtttcacc agttcatact ggctggtgtt ggtgtcctga
    40201 tattcgtcca ccagcaggta gcgaattttg ttctgccagc gctcgcggac ttcttcattg
    40261 cgttgcagaa gtagcgtcgg caataaaatc agatcatcga agtcgagcac attacaggct
    40321 ttcaggtgtg catcatacag cccataacaa tgggcaaaaa tacggtcccg ctcgccaatc
    40381 gcacttgctg ccgcctggga cggtgttttg agatcattct tccagttaga gatggtcgaa
    40441 atcagttgtt gcaggagaac tttgtcatct tcaatcagcc cctcggtcaa ctctttaagc
    40501 aaagcaagct gatcggtatc gtcaaacagc gaaaagttcg ccttcatccc aagcgccgca
    40561 tactcgcgtt tgatgatatc caaccccagc gtgtgaaaag tggagatcat cagcccgcgc
    40621 gcctctttgc gacccagcgt ctgcccgaca cgctctttca tctcgcgcgc cgccttattg
    40681 gtaaaggtca ccgccgcgat atgtcgcgcc tgataaccgc aaccgcggat cagatgggcg
    40741 attttattgg tgatcacacg agttttaccg gaacccgcgc ccgccagcac caggcagggg
    40801 ccggtaacga attcgacagc ttgttgttgg ccggggttta gacgcatagg tgtattgctc
    40861 aatcttcgaa cggggggagg attgtagcag aatcgcattt gaatgtgacc gcgtgtaagc
    40921 cgctaaccgg ttggctgcgg atgtttaaat cagagagaga agaccgaaac cggagttcat
    40981 cgtgaatcca ttagacttag aaaatatcgg gtcgtaacgt tgtgttatta ctttttcaga
    41041 gtttactaag gagaggtgta ctcgtggcga ttaatttcag tcctaaggtt ggtgaaatac
    41101 tggaatgcaa ctttggtaac tatcctgtta gccagaatgg acagttcagc accaagtatt
    41161 acgatggccg cattcctcct gaaatgataa aaaacaggtt agttgtcgtg ctcaacggaa
    41221 aaattaatgg caacgcatgt atcgtcgttc cattatcaac cactcgcgat catgacaagt
    41281 taaatcgtgg aatgcatgtt gaaatagcca gtaatgttat caatggcctg caattctttg
    41341 atcagcagat tcgttgggcc aaagccgacc tggtacaaca agtcagtcga aaccgattaa
    41401 atagagcaag gacttacaga gggtatctta atcaatgttt gccacatgag ttggttgcag
    41461 acatacagag ggcggtcatc aagtcaatca atgccatatc gctcataaat tgacaataaa
    41521 atcaccatcc ccgataatga ctttgtagcc catagaggcc ctaagttttc tccccttgag
    41581 ggacctgcga agcccggatt ttccgggctt ttgccatata tgtaccagcc cctccctgac
    41641 tgacaattac gttagcatct gcggatcatg ctaaaatcgc cgcctgacaa ttattcactc
    41701 aaggcacgat catggcaaaa acagcagcag cactgcatat ccttgtaaaa gaagagaaac
    41761 tggctctgga tcttctcgag cagattaaga acggtgccga tttcggcaag ctggcgaaga
    41821 aacactccat ttgcccatca ggcaaacgcg gcggtgattt aggtgaattc cgccagggtc
    41881 agatggttcc ggcgttcgat aaagttgtgt tctcttgtcc ggtactggag ccaaccggcc
    41941 cgctgcacac ccagttcggt taccacatca ttaaggtgct gtaccgtaac taatagcaag
    42001 gccttctcca ggagaaggcc ttgagtgttt tctccctctc cctgtgggag agggtcgggg
    42061 tgagggcatc agcgcgcact taacccgcaa ctgcaatacg tttcatatcc gtcatatagc
    42121 cgcgcagttt cttacctacc tgctcaatcg catggcagcg aatcgcttcg ttcacatcac
    42181 gcagttgcgc gttatctacc gcaccttccg gaatagcttt acccagatcg cccggttgca
    42241 gctctgccat aaacggtttc agcagcggca cacaagcgta agagaacaga tagttaccgt
    42301 actcggcggt atcagagata accacgttca tttcgtacag acgcttacgg gcgatggtgt
    42361 tggcaatcag cggcagttcg tgcagtgatt catagtaagc agattcttcg atgatgccgg
    42421 aatcaaccat ggtttcaaac gccagctcaa cgcccgcttt caccatcgcg atcatcagta
    42481 cgcctttatc gaagtactcc tgctcgccga ttttgccttc atactgcggc gcggtttcga
    42541 aggcggtttt gccggtctct tcacgccagg tcagcagttt cttatcatcg ttggcccagt
    42601 ccgccatcat accggaggag aattcgccgg agatgatgtc gtccatatgt ttctggaaca
    42661 ggggtgccat gatctctttc agctgttcag acagcgcgta agcacgcagt ttcgccgggt
    42721 tagagagacg gtccatcatc agggtgatgc cgccctgctt cagcgcttcg gtgatggttt
    42781 cccaaccgaa ctgaattagt ttttctgcgt atgccggatc ggtgccctct tccaccagct
    42841 tgtcgaagca cagcagcgag ccagcctgca acataccgca caggatggtt tgctcaccca
    42901 tcaggtcaga tttcacttcc gcaacgaagg acgattccag cacgcccgca cggtgaccac
    42961 cggttgctgc cgcccatgct ttagcgatcg ccataccttc gcctttcgga tcgttttccg
    43021 ggtgaacggc aatcagcgtc ggtacgccga atccacgttt gtactcttca cgcacttcgg
    43081 tgccagggca tttcggcgca accatgacta cggtgatatc tttacggatc tgctcgccca
    43141 cttcgacgat gttgaaaccg tgcgagtaac ccagcgccgc gccgtctttc atcagtggct
    43201 gtacggtgcg cactacatca gagtgctgct tgtccggcgt caggttaacc accagatccg
    43261 cctgcgggat cagttcttcg taagtaccaa ctttaaaacc gttttcggtc gctttacgcc
    43321 aggacgcgcg cttctcggca atcgcttctt tacgcagagc gtaggagata tcgagaccag
    43381 aatcacgcat gttcaggccc tggttcagcc cctgtgcgcc acagccgacg ataaccactt
    43441 ttttaccctg aaggtagctc gcgccgtcgg cgaattcgtc gcgccccata aagcgacatt
    43501 tgcccagctg tgccagctgc tggcgcaggt tcagtgtatt gaagtagtta gccatggtga
    43561 ttcctcgtga tgttgtgctt cttattgttt ggttcgcgtt tgcgagattg aattcactat
    43621 atgacaggaa atttattgcg gaaattgata tattcacaac gtcacattgc aatttctgca
    43681 acgtcaacat tgagggctgc gccattggat ttacgcgatc tgaaaacctt cctgcatctg
    43741 gcggaaagcc gccattttgg ccgcagcgcg cgggcgatgc acgttagccc gtccacgctc
    43801 tcacggcaga ttcagcgcct ggaagacgat ctcggtcagg cattatttgt gcgcgataac
    43861 cggacggtga cgctgaccga agcgggcgaa gagttacgcg ttttcgccca gcaaacattg
    43921 ttgcagtatc agcaactgcg ccacaccatc gatcagcaag ggccgtcgct ctctggcgag
    43981 ttacatattt tctgctcggt gaccgccgct tacagccatc taccgccgat tctcgaccgc
    44041 ttccgcgcgg aacacccgtc ggtggagatt aaacttacta ctggtgatgc ggcagatgcg
    44101 atggaaaagg tggtcactgg tgaagcggat ctggcgattg cgggtaaacc ggaaaccttg
    44161 cccggcgcag tggcgttttc gatgctggag aatctggcag tagtgctgat tgcccccgcg
    44221 ctgccctgcc cggtgcgtaa tcaggtgtcg gtagagaagc cggactggtc aacggtgccg
    44281 tttatcatgg ccgatcaggg gccggtacgc cgccgcattg aactgtggtt tcggcgtaac
    44341 aaaatcagta acccgatgat ttacgccacg gttggcgggc atgaagcgat ggtatcgatg
    44401 gtggcgctcg gctgtggcgt ggcgctgttg ccggaagtgg tgctggaaaa cagcccggaa
    44461 ccggtgcgta accgcgtgat gatactagag cgaagcgatg agaaaacgcc gtttgagctg
    44521 ggcgtgtgtg cgcagaaaaa gcggctgcat gagccgctga ttgaagcgtt ctggaagatt
    44581 ttaccgaacc actaacccgc caaaaagaac ctgaacgccg ggttattggt ttcgtcgtgg
    44641 caatcgtagc ccagttcatt cagccgggtt tcgaaatccg gttcatggtc gccaagctcg
    44701 aacgccgcca gtacgcgccc gtagtcggta ccgtgactgc gataatggaa cagagaaata
    44761 ttccagtgcg tacccagcgt gttgaggaag cgcagcagcg cacccggcga ttccggaaat
    44821 tcgaagctgt agagacgttc ctgcaacgga tgcgatggac gcccgccaac catatagcgc
    44881 acatgtagct tcgccatttc atcgtcggag agatcaacca cgctgtagcc gccgtcgttg
    44941 agcatctgca aaatttcttt gcgctcttcg aggccgcggc ttaagcgcac gccgacaaag
    45001 atgcaggcgt ttttggcatc ggcaaagcgg tagttgaatt cggtgaccga acgtccgcca
    45061 agcaactggc agaatttgag gaagctacct ttctcttccg gaatggttac cgccagcaac
    45121 gcttcacgct gttcacccag ctcacaacgt tccgagacgt agcgcaggcc gtgaaagttc
    45181 acgttagcac cggaaagaat atgcgccagc cgttcgccgc gaatgttgtg ctgggcgata
    45241 tattttttca ttcccgccag cgccagcgcg ccagagggtt ccgccaccgc acgcacatct
    45301 tcgaataaat ccttcatcgc cgcacagatc gcatcgctat cgacggtgat gatgtcgtcg
    45361 agatactcct ggcataaacg gaaggtttcg tcaccgatgc gttttaccgc tacgccttca
    45421 gcaaatagcc ctacgcgcgg cagatcaacc ggatgacccg catccagcgc tgctttcagg
    45481 caggcggagt cttccgcttc tacggcgatc actttgattt gtggcatcag ttgtttgatc
    45541 agcaccgcaa cgcccgccgc cagaccgccg ccgccgactg gcacaaatac gcggtcgaga
    45601 tgggcgtcct gctggagcag ttccagcgcc agcgtgcctt gcccggcaat caccatcgga
    45661 tggtcgaacg gcggcaccca ggtgaacccc tgctgctgtg acagttcgat cgctttagct
    45721 ttcgcttcgt caaagttcgc gccatgaagc agcacttcgc cgccgaatcc acgtaccgca
    45781 tcgactttga tgtcggcggt ggcggttggc ataacgatca gggccttcac gcctaaccgc
    45841 gcagaagaaa acgcgacgcc ctgcgcgtgg ttacccgcag aagcagtgat cacgccgtgc
    45901 gctttttgtt cttccgtcag gcccgccatc atggcgtatg caccgcgcag cttaaagctg
    45961 tgcactggct ggcgatcttc gcgcttcacc agaatgacgt tatcaagacg cgacgacagt
    46021 ttttccattt tttgtagcgg cgtaacctgc gccgcctcgt aaaccggcgc gcgcagcact
    46081 gctcttaaat attcggcacc ttccggagca ccggacaggg gttgcgagtc agccattatt
    46141 aaccccccag tttcgattta tcgcgcaccg cacctttgtc ggcgctggtt gccaggctgg
    46201 cgtaggcgcg cagggcaaag gaaacctgac gttcacggtt tttcggcgtc caggctttgt
    46261 caccccgagc ttcctgcgct tcacgacgcg ccgccagttc ggcatcgctt acctgtaact
    46321 gaatgccacg gttcgggatg tcgatagcga tcaggtcacc atcttcaatc aggccaatgc
    46381 tgccgccgct tgccgcttcc ggtgagacgt ggccgatgga aagaccagag gtgccgccag
    46441 agaaacgacc gtcggtgatc agcgcacagg ctttaccgag acccattgat ttcaggaagc
    46501 tggttgggta gagcatttcc tgcatgccag ggccgccttt cgggccttca tagcggatca
    46561 ccaccacatc gcccgcgaca actttgccac cgaggatcgc ttctaccgcg tcgtcctggc
    46621 tttcgtacac tttcgccggg ccggtgaatt tgaggatgct gtcatcgacg cccgcggttt
    46681 taacgatgca gccgttttct gcgaaattac cgtagagcac cgccaggccg ccgtctttgc
    46741 tgtaggcgtg ttccagcgag cggatacagc cattggcgcg atcgtcatcg agagaatccc
    46801 aacggcaatc ctgcgagaat gcctgtgtgg tacgaatgcc tgcaggacct gcgcggaaca
    46861 tattttttac cgcgtcatcc tgggtcagca taacgtcgta ttgttccagc gtttgcggca
    46921 acgtcaggcc aagtacgttt ttcacatcac ggttcagtaa ccccgcgcga tccagttcgc
    46981 cgagaatacc gataacacca ccagcacggt gaacatcttc catatggtat ttctgggtgc
    47041 tcggcgcaac tttacacagc tgtggaacct tgcgggaaag cttatcgata tcactcatgg
    47101 tgaagtcgat ttccgcttcc tgcgccgccg ccagcaggtg aagtacggtg ttagtcgatc
    47161 cacccatcgc gatatccagc gtcatggcgt tttcaaacgc cgctttgcta gcaatattgc
    47221 gcggcagtgc actttcgtcg ttttgctcgt agtaacgttt ggtcaattca acaatgcgtt
    47281 taccggcatt gaggaacaac tgcttacggt ccgcgtgggt tgccagcagc gagccgttgc
    47341 ccggctgcga caggcccagc gcttcggtca ggcagttcat tgagttagcg gtaaacatcc
    47401 cggagcagga gccgcaggtc ggacacgcgg aacgttcaac ctgatcgctc tgggagtcag
    47461 aaactttcgg gtctgcgccc tggatcatcg catcaaccag atcgagtttg attatctgat
    47521 cggacagttt ggttttcccg gcctccatcg ggccgccgga aacaaagatc accggaatat
    47581 tcaggcgcag ggaagccatc agcatccccg gggtgatttt gtcgcagtta gagatgcaga
    47641 ccatggcgtc ggcgcagtgg gcgttgacca tatactcaac ggaatcagcg atcagttcgc
    47701 gagatggcag tgaataaagc atccccccgt ggcccatggc aatcccatca tccaccgcaa
    47761 tggtgttgaa ctctttggca acgccgccag ccgcttcaat ttgttcggcg accagtttac
    47821 cgagatcgcg cagatggacg tgacccggta caaattgggt gaacgagttc acaaccgcga
    47881 taatcggctt gccaaaatcg gcgtcggtca ttccggtggc gcgccacagc gcacgagcac
    47941 ccgccatatt acgaccatga gtggtggtgg cggaacggta cttaggcata ctttatttac
    48001 tcccagtgtc tgtctcgtaa atgggacggt gcgtgccgtc ccattttttg tatttattga
    48061 ttaacttgat ctaaccagcc ccatttatct tcggtttcgc cagtgaaaag gccgaagaag
    48121 gcttgctgaa tgcgtttggt aaccgggcca caacggcctt cgccaacctg aataccgtct
    48181 acgctgcgca ctggcgtgat ttctgccgca gtaccggaca taaacacttc atccgccagg
    48241 tacagggatt cgcgcgacag aacctgctca cgcacttcaa ttcccagctc tttcgccagt
    48301 ttgatgatgg catcacgggt aatacccggc agcgcggagg aggtgaacgg tggggtgaac
    48361 agcacaccat ctttcacttc aaacaggttt tcgcctgcgc cttcagagat ataaccgttc
    48421 acatccagcg cgataccttc ctgataaccg tggcggcgcg cttcgctacc caccagcagg
    48481 gaagagagat agttaccacc ggcttttgcc gcggttggga tggtgtttgg tgctgcgcgg
    48541 ttccaggagg aaaccatcgc atcgatcccc tgctccagcg cttctgcgcc cagatacgct
    48601 ccccacggga aagcggcgat aatcacatcg gttgagtatc ccgctggcgg gtttacaccc
    48661 atgccaacat caccaacgaa gatcagcgga cggatatagg cgctggtgag attgtttttg
    48721 cggatcacgt cacgacaagc ttccatcagc tcatcaatgc tctgcgaaac cgggaagcga
    48781 tagattttgg cggagtcatg cagacgctgc atatgctcac gatggcggaa tacaaccggt
    48841 cctttgtgcg agtcgtagca acggatgcct tcaaaaaccg aagtgccata gtgcagcgcg
    48901 tgcgacatca catgcacctt cgcgtcttcc cagcgaacca tctccccatt gaaccaaatg
    48961 taatcagctt tcttcgtggt catttttata ttccttttgc gctcaggcgc ggatttgttg
    49021 tgatgtggtt gtgctctggc agatggcaac gtgtgcgacg tccaccagtt tatttaactg
    49081 actaaacagt aagtcgaccg accgtgggct ggcaacggtc aattcgatat ttatattttg
    49141 tgcatcgctg gcggcagcca tattcattga gcagacgtgg aaaccacgat gacgcaccac
    49201 gcgtaaaaca cgttctaagg tttccggatt gaagcgagcc gatacattga cctgatgttg
    49261 catcatgata atttctccaa catttctgaa ttactggcgc caggcggcac cagcggccag
    49321 acgttctcaa gttcgtcgat tgagacatga agcaggtatg gcccatcact gttcagcatg
    49381 gtgtcgagtg ccgcttcaac ctggtcttta cgggtgatgt gttggccagg gatgccgaag
    49441 gcgctggcta acatgaggaa atcggggtta tcagtaaggg tggtttcgct gtatcgttcc
    49501 tgaaaaaaca gttgctgcca ttgtcgaacc atccctaacc gttggttatc gagtaagacg
    49561 attttcaacg gtaactgctt gcgttttacg gtgcccagct cttgcacatt catcatgaaa
    49621 gagccgtcac cggagataca gacgacagta tcgttcggtc gtgcgacttg tgcgccaacc
    49681 gccgctggta aaccgaaacc catggtgcct aagccgctgg aggtaatgaa attttccggg
    49741 cgagtgtgtg cgatgtgctg cgcggcccac atctggtgct gccccacatc tgtggtcacg
    49801 acgcaatccg caggtttacg atccgacagt tgttttaaca acaacggcgc gtagatagcg
    49861 tcaccgggat ggtcgtaacg ccaggtatgt tcatcacgca gctgcgcgca gtattgctgc
    49921 cagtcattga tatttaacgg ctgctgtaat gctggtaaca gagcatttaa atcaccttgt
    49981 aatgccacat gtgcctgacg cagcttgttc atttctgccg ggtcgatatc catatggata
    50041 acactggcgt gtggtgcgaa ggtgttcagt ttgccggtca cccggtcatc aaaacgtgca
    50101 cccacggcga tcagcaagtc gcactcctgc accgcgaagt tcgccgcttt ggtgccatgc
    50161 attcccagca tgcccaggta gtacggataa tctgcttcaa ctgcgcccag ccctttcagc
    50221 gtgcaggtgg caggcatttt tgtggtagcg agaaattctc gtaaagcagg aactgcctgc
    50281 gccataccca cgccaccacc aacgtacagc atcggttttt gcgcttttgc cagcatctgg
    50341 cgcgcttgct caacttcggc atgggggaaa gtcacttcgt tttcaacggt ggtgaaccac
    50401 ggttccagat cgccgctggc taactggata tcttttggga tatcgaccag aaccggacca
    50461 ggacgacctg agctggcaac gtcgaatgct gcagccatga tgcgcggcaa ctcttccagc
    50521 gactgcacca gaaagctgtg cttggtacag gctaacgaca atcccaggac atccacttcc
    50581 tgaaatgcgt cggtgccgat aaacggtgcg gacacttgac cggtgatggc aacgacaggg
    50641 atggaatcta acagtgcgtc cgcaagcccg gttatcaggt tggttgcgcc cggaccagac
    50701 gtggcgatac atacgccagt tttaccggta gcacgggcat aaccgatagc cgccattgcc
    50761 gcaccctgct catgtcggca cagcaagtgc tccacgccgc cgtcatacaa tgcatcgtaa
    50821 accggcataa ttgcgccacc cggataaccg aaaacggtgt tcacaccctg tgcccgcaac
    50881 gcatgtacca cccactgtgc gccattcata gttagttccc cgtcctgaat cttgagaaac
    50941 agaattttgt gctgttattc attgtctgct cctcggttat gtttttaagg tcaaaaaaaa
    51001 cccccggacc tttcggtgcg ggggtcttag ttcgttaagg cttgatttct aagcctttcc
    51061 tcgtccaagt gcagccccgc acggtgggat aataatcacc accacgctaa tcacgaccag
    51121 gctaatcact cgtagaaggg ctgtcatttt gtcttttctt gcatcttgtt cgaaggaatg
    51181 cctaaagagt taccataggt tttgtaaata gtacaagata ttttttggcc gatttttatg
    51241 atgcgtttaa taatttcaat ataaattgtt gttttttaaa aataaaatag tgagtgaaaa
    51301 aaattcaatt tcagtatgaa tgttgttccc ttcctcgtag ttcatccttt gtccgctgca
    51361 tttttgcgag catcattcca ccgccagaac aactcaactt cacttgcaca aataaacagg
    51421 aatcagccac taaaattcac gaattagcgg gctacagcag cgatatctta tgcacataat
    51481 ggtgactcaa ggagggctta tgtcactgtc aattgttcat acccgcgcag ccctgggagt
    51541 aaatgcgccc ccgatcactg ttgaggtaca tatcagtaaa ggtctacccg gcttaacgat
    51601 ggtgggctta ccagaaacaa cggtaaaaga agctcgcgat cgcgtgcgca gcgccattat
    51661 caatagcgga tatgaatatc cggcgaaaaa aatcaccatc aacctggctc cagccgatct
    51721 gccaaaagaa gggggacgat atgatttacc tatcgccatt gcgttgctgg cggcctcaga
    51781 acagcttaca gccaataagt tagatgaata tgaattagtc ggagaactgg ctcttacagg
    51841 cgctctgcgt ggcgttcccg gtgcaatctc cagtgcaact gaagctatta agtcgggcag
    51901 aaaaattatc gtcgcgaaag ataacgaaga tgaagtgggg ctaattaacg gtgaaggatg
    51961 cctgatagcc gatcatctgc aagctgtctg tgcgtttctg gaaggtaagc acgctctcga
    52021 acgcccgaaa ccaactgatg cagtatcccg ggcgctacaa catgatctca gtgatgttgt
    52081 cggtcaggaa caaggaaagc gaggactgga aattaccgcc gctggcgggc acaacctttt
    52141 actgattggg ccgccgggaa caggtaaaac aatgctcgcc agccgtatta atggcctgtt
    52201 gccagattta agcaatgaag aggcactgga gagcgctgcg atattaagtc tggtaaatgc
    52261 tgaatcagta caaaaacaat ggcggcagcg cccgttccgc tcacctcatc acagtgcgtc
    52321 gttaactgcg atggtggtcg gtggtgcaat tccagggcca ggtgaaattt cgttggcgca
    52381 taacggcgtg ctttttcttg atgagctacc tgaatttgaa cggcgtacac tggatgcctt
    52441 gcgagagccg attgaatccg ggcagatcca tctttcacgc acacgagcaa aaataaccta
    52501 tccagcccgt ttccagcttg ttgcggcgat gaatcccagc cctaccggac attatcaggg
    52561 aaaccataac cgctgcacgc cagaacagac attacgttat ctcaaccggc tctcggggcc
    52621 ctttctcgac cgcttcgatc tctcactgga gatcccatta ccaccccccg gcattttgag
    52681 taaaacggta gtgccgggag aaagcagcgc caccgttaaa caacgcgtaa tggccgccag
    52741 agagcgccaa tttaagcggc agaataaact gaatgcctgg ctggatagtc cggaaatacg
    52801 ccaattctgc aaacttgaga gcgaagatgc gatgtggttg gaaggaacac tgatccatct
    52861 ggggttatcg attcgtgcct ggcagcggtt attgaaagtt gcacgaacca ttgctgatat
    52921 tgatcagtct gacattatca cacgtcagca tttgcaggag gcagttagct atcgtgcgat
    52981 tgaccgtttg ctcatccatc tgcaaaaact actgacataa aaaaagggca tttcgccctt
    53041 tttattaatc atcagaatcg gtgtagtctt cagcaccttc aacctgcggt ttaccgccgg
    53101 aaagggtgtg aaaacgtttt ggacgcttaa tacgcgtcat atacttggac cacacgcgtt
    53161 ctgcttctgt cactggctca cgttcgccac ggcatactgc tacgaagagt ttctcttcct
    53221 cggtaaccgg ctcgcgtttg ccaagatcca actcattgaa ggcataacca tgacgctcaa
    53281 gcagttgtgc ctctttgatg gtgaaatcac catgacgaga gaatccacgt ggataatgtt
    53341 tattgtcgaa atatcgatta gtcgtcgtaa agctttccgc catcctgcac gctcctaatt
    53401 ctttgaccga gctagttatg gcgcggagta ttagttacgc ttgacagagt gtaaaacaaa
    53461 acatttaaat cataacgaca aataattttg cggagagcac tgtggatacg gaattgttaa
    53521 aaactttcct ggaagttagc cgaacgcgtc actttggtcg agcggctgaa tcgctctatc
    53581 tgacccagtc agcagtgagc tttcgaatca gacaactgga aaatcaactg ggtgtgaacc
    53641 ttttcacccg ccacagaaac aatatccgtt taaccgctgc cggtgaaaaa ctactgcctt
    53701 atgcagaaac gctcatgagc acctggcagg ccgcccgtaa ggaggtggcg catacctcac
    53761 gacataacga gttttctatc ggtgccagcg cctcgttgtg ggaatgtatg cttaatcagt
    53821 ggctgggacg cttgtatcaa aatcaggatg cccatacagg cttacagttc gaagcgcgaa
    53881 ttgcccaacg gcagtctctg gtaaaacagc tgcatgaacg ccagcttgat cttcttatta
    53941 ccactgaagc gcccaaaatg gacgaattta gtagtcagtt gctgggatat ttcactttag
    54001 cgctttatac cagtgcccct tcaaaactaa agggagatct taattatctg cgacttgagt
    54061 gggggccaga ttttcaacag catgaggcag gtttgatcgg tgctgacgaa gtgcccattc
    54121 tgacaaccag ttctgctgaa ctggcacagc aacagattgc gatgcttaat ggttgcacct
    54181 ggctacccgt cagctgggcg cgtaaaaaag gcggcctgca taccgttgtc gatagcacaa
    54241 cactttcacg gccgctttat gccatatggc tgcaaaatag cgataaaaat acgttgattc
    54301 gcgatctttt gaaaattaac gtgctggatg aagtgtatta atatgaatgg ctggcaagga
    54361 tgccggtaga aggatttact tcggagaggg ttatttcaga taaaaaaaat ccttagcttt
    54421 cgctaaggat gatttctggc aggggcggag agactcgaac tcccaacacc cggttttgga
    54481 gaccggtgct ctaccaattg aactacgccc ctaattaggg tggcggaacg gacgggactc
    54541 gaacccgcga ccccctgcgt gacaggcagg tattctaacc gactgaacta ccgctccacc
    54601 gaattctttt acaaccaccg gttttatgac cggcttac
//
LOCUS       DAZGVQ010000011        51565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000011 DAZGVQ010000000
VERSION     DAZGVQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.011"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(63..1427)
                     /gene="mnmE"
                     /locus_tag="ACHLAG_000629"
     CDS             complement(63..1427)
                     /gene="mnmE"
                     /locus_tag="ACHLAG_000629"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFP0653919.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALAQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(1533..3179)
                     /gene="yidC"
                     /locus_tag="ACHLAG_000630"
     CDS             complement(1533..3179)
                     /gene="yidC"
                     /locus_tag="ACHLAG_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFP0653920.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3182..3439)
                     /gene="yidD"
                     /locus_tag="ACHLAG_000631"
     CDS             complement(3182..3439)
                     /gene="yidD"
                     /locus_tag="ACHLAG_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFP0653921.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3403..3762)
                     /gene="rnpA"
                     /locus_tag="ACHLAG_000632"
     CDS             complement(3403..3762)
                     /gene="rnpA"
                     /locus_tag="ACHLAG_000632"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP0653922.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3779..3919)
                     /gene="rpmH"
                     /locus_tag="ACHLAG_000633"
     CDS             complement(3779..3919)
                     /gene="rpmH"
                     /locus_tag="ACHLAG_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFP0653923.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4149..4229)
                     /locus_tag="ACHLAG_000634"
     CDS             complement(4149..4229)
                     /locus_tag="ACHLAG_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0653924.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            4526..5929
                     /gene="dnaA"
                     /locus_tag="ACHLAG_000635"
     CDS             4526..5929
                     /gene="dnaA"
                     /locus_tag="ACHLAG_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFP0653925.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            5934..7034
                     /gene="dnaN"
                     /locus_tag="ACHLAG_000636"
     CDS             5934..7034
                     /gene="dnaN"
                     /locus_tag="ACHLAG_000636"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFP0653926.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7034..8107
                     /gene="recF"
                     /locus_tag="ACHLAG_000637"
     CDS             7034..8107
                     /gene="recF"
                     /locus_tag="ACHLAG_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFP0653927.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8136..10550
                     /gene="gyrB"
                     /locus_tag="ACHLAG_000638"
     CDS             8136..10550
                     /gene="gyrB"
                     /locus_tag="ACHLAG_000638"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFP0653928.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            10790..11188
                     /gene="yidB"
                     /locus_tag="ACHLAG_000639"
     CDS             10790..11188
                     /gene="yidB"
                     /locus_tag="ACHLAG_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFP0653929.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            11303..12115
                     /gene="yidA"
                     /locus_tag="ACHLAG_000640"
     CDS             11303..12115
                     /gene="yidA"
                     /locus_tag="ACHLAG_000640"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFP0653930.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12161..12817)
                     /gene="yidX"
                     /locus_tag="ACHLAG_000641"
     CDS             complement(12161..12817)
                     /gene="yidX"
                     /locus_tag="ACHLAG_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFP0653931.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13095..13784
                     /gene="dgoR"
                     /locus_tag="ACHLAG_000642"
     CDS             13095..13784
                     /gene="dgoR"
                     /locus_tag="ACHLAG_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFP0653932.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            13781..14659
                     /gene="dgoK"
                     /locus_tag="ACHLAG_000643"
     CDS             13781..14659
                     /gene="dgoK"
                     /locus_tag="ACHLAG_000643"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFP0653933.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDSRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGDQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            14643..15260
                     /gene="dgoA"
                     /locus_tag="ACHLAG_000644"
     CDS             14643..15260
                     /gene="dgoA"
                     /locus_tag="ACHLAG_000644"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFP0653934.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            15257..16405
                     /gene="dgoD"
                     /locus_tag="ACHLAG_000645"
     CDS             15257..16405
                     /gene="dgoD"
                     /locus_tag="ACHLAG_000645"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFP0653935.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHVDNSVAEW"
     gene            16480..17817
                     /gene="dgoT"
                     /locus_tag="ACHLAG_000646"
     CDS             16480..17817
                     /gene="dgoT"
                     /locus_tag="ACHLAG_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0653936.1"
                     /translation="MVSGFAMPKIWIKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYYIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17814..18878)
                     /gene="cbrA"
                     /locus_tag="ACHLAG_000647"
     CDS             complement(17814..18878)
                     /gene="cbrA"
                     /locus_tag="ACHLAG_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFP0653937.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPTSVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            18979..20193
                     /gene="yidR"
                     /locus_tag="ACHLAG_000648"
     CDS             18979..20193
                     /gene="yidR"
                     /locus_tag="ACHLAG_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFP0653938.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(20195..20527)
                     /gene="yidQ"
                     /locus_tag="ACHLAG_000649"
     CDS             complement(20195..20527)
                     /gene="yidQ"
                     /locus_tag="ACHLAG_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP0653939.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    20766..20838
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            20832..21245
                     /gene="ibpA"
                     /locus_tag="ACHLAG_000650"
     CDS             20832..21245
                     /gene="ibpA"
                     /locus_tag="ACHLAG_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFP0653940.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            21357..21785
                     /gene="ibpB"
                     /locus_tag="ACHLAG_000651"
     CDS             21357..21785
                     /gene="ibpB"
                     /locus_tag="ACHLAG_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFP0653941.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISECPALNS"
     gene            21982..23643
                     /gene="yidE"
                     /locus_tag="ACHLAG_000652"
     CDS             21982..23643
                     /gene="yidE"
                     /locus_tag="ACHLAG_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFP0653942.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(23640..24356)
                     /gene="yidP"
                     /locus_tag="ACHLAG_000653"
     CDS             complement(23640..24356)
                     /gene="yidP"
                     /locus_tag="ACHLAG_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0653943.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPVIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            24652..26268
                     /locus_tag="ACHLAG_000654"
     CDS             24652..26268
                     /locus_tag="ACHLAG_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP0653944.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEAFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            26268..27082
                     /locus_tag="ACHLAG_000655"
                     /pseudo
     CDS             26268..27082
                     /locus_tag="ACHLAG_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27079..27972)
                     /gene="yidL"
                     /locus_tag="ACHLAG_000656"
     CDS             complement(27079..27972)
                     /gene="yidL"
                     /locus_tag="ACHLAG_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP0653945.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            28139..29854
                     /gene="yidK"
                     /locus_tag="ACHLAG_000657"
     CDS             28139..29854
                     /gene="yidK"
                     /locus_tag="ACHLAG_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFP0653946.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            29851..31344
                     /gene="yidJ"
                     /locus_tag="ACHLAG_000658"
     CDS             29851..31344
                     /gene="yidJ"
                     /locus_tag="ACHLAG_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP0653947.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVALGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(31391..31840)
                     /gene="yidI"
                     /locus_tag="ACHLAG_000659"
     CDS             complement(31391..31840)
                     /gene="yidI"
                     /locus_tag="ACHLAG_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFP0653948.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            31950..32297
                     /gene="yidH"
                     /locus_tag="ACHLAG_000660"
     CDS             31950..32297
                     /gene="yidH"
                     /locus_tag="ACHLAG_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFP0653949.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            32287..32649
                     /gene="yidG"
                     /locus_tag="ACHLAG_000661"
     CDS             32287..32649
                     /gene="yidG"
                     /locus_tag="ACHLAG_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFP0653950.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            32646..33143
                     /gene="yidF"
                     /locus_tag="ACHLAG_000662"
     CDS             32646..33143
                     /gene="yidF"
                     /locus_tag="ACHLAG_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFP0653951.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRAVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(33151..34335)
                     /gene="emrD"
                     /locus_tag="ACHLAG_000663"
     CDS             complement(33151..34335)
                     /gene="emrD"
                     /locus_tag="ACHLAG_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFP0653952.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSDQGQ
                     PV"
     gene            34417..34491
                     /gene="ysdE"
                     /locus_tag="ACHLAG_000664"
     CDS             34417..34491
                     /gene="ysdE"
                     /locus_tag="ACHLAG_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFP0653953.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            complement(34754..34843)
                     /gene="tisB"
                     /locus_tag="ACHLAG_000665"
     CDS             complement(34754..34843)
                     /gene="tisB"
                     /locus_tag="ACHLAG_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFP0653954.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            35408..35506
                     /gene="ivbL"
                     /locus_tag="ACHLAG_000666"
     CDS             35408..35506
                     /gene="ivbL"
                     /locus_tag="ACHLAG_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFP0653955.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            35612..37300
                     /gene="ilvB"
                     /locus_tag="ACHLAG_000667"
     CDS             35612..37300
                     /gene="ilvB"
                     /locus_tag="ACHLAG_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFP0653956.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            37304..37594
                     /gene="ilvN"
                     /locus_tag="ACHLAG_000668"
     CDS             37304..37594
                     /gene="ilvN"
                     /locus_tag="ACHLAG_000668"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP0653957.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            37668..38258
                     /gene="uhpA"
                     /locus_tag="ACHLAG_000669"
     CDS             37668..38258
                     /gene="uhpA"
                     /locus_tag="ACHLAG_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFP0653958.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            38258..39760
                     /gene="uhpB"
                     /locus_tag="ACHLAG_000670"
     CDS             38258..39760
                     /gene="uhpB"
                     /locus_tag="ACHLAG_000670"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFP0653959.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            39770..41089
                     /gene="uhpC"
                     /locus_tag="ACHLAG_000671"
     CDS             39770..41089
                     /gene="uhpC"
                     /locus_tag="ACHLAG_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFP0653960.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            41227..42618
                     /gene="uhpT"
                     /locus_tag="ACHLAG_000672"
     CDS             41227..42618
                     /gene="uhpT"
                     /locus_tag="ACHLAG_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFP0653961.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(42663..44429)
                     /gene="adeD"
                     /locus_tag="ACHLAG_000673"
     CDS             complement(42663..44429)
                     /gene="adeD"
                     /locus_tag="ACHLAG_000673"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFP0653962.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQLLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            44604..45938
                     /gene="adeQ"
                     /locus_tag="ACHLAG_000674"
                     /gene_synonym="yicO"
     CDS             44604..45938
                     /gene="adeQ"
                     /locus_tag="ACHLAG_000674"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFP0653963.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            45991..46443
                     /gene="yicN"
                     /locus_tag="ACHLAG_000675"
     CDS             45991..46443
                     /gene="yicN"
                     /locus_tag="ACHLAG_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFP0653964.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            46654..47844
                     /gene="nepI"
                     /locus_tag="ACHLAG_000676"
     CDS             46654..47844
                     /gene="nepI"
                     /locus_tag="ACHLAG_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFP0653965.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(47885..48178)
                     /gene="yicS"
                     /locus_tag="ACHLAG_000677"
     CDS             complement(47885..48178)
                     /gene="yicS"
                     /locus_tag="ACHLAG_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFP0653966.1"
                     /translation="MKPTTLLLIFTFFAMLGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            48400..49218
                     /gene="nlpA"
                     /locus_tag="ACHLAG_000678"
     CDS             48400..49218
                     /gene="nlpA"
                     /locus_tag="ACHLAG_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFP0653967.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(49222..50145)
                     /gene="yicL"
                     /locus_tag="ACHLAG_000679"
     CDS             complement(49222..50145)
                     /gene="yicL"
                     /locus_tag="ACHLAG_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFP0653968.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(50256..51440)
                     /gene="setC"
                     /locus_tag="ACHLAG_000680"
     CDS             complement(50256..51440)
                     /gene="setC"
                     /locus_tag="ACHLAG_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFP0653969.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT        12000 a        13043 c        13874 g        12648 t
ORIGIN      
        1 aggagtgcta acaatgaagc gaagccgcat ctgacagtca gaatacggct tcgtaagcgc
       61 ggttacttac caatacagaa gctggagaaa atccgcccca gcaggtcgtc tgaagtaaat
      121 tccccggtga tttcgcttaa gttctgctgt gccagacgca actcttccgc cagcagttca
      181 cctgcccagg ctcccaacag ttgcgctttg ccctgttgta gatgttccgc tgcctgtgcc
      241 agcgcctgta ggtggcgacg acgcgccagg aagccgcctt ccatgttggt gtcaaagccc
      301 atgctctgtt tgagatggtt acgcagcacg tccacgcctt caccagtcct tgccgagaga
      361 cgaattaacg cgtgaccgtt cacttcactc attcccagcg tttcgccggt gatatcggct
      421 ttattgcgca ccacggtgat cggcagtttc gctggcagac gggcaataaa ttccggccag
      481 atctctgccg gatccacggc gtctgttgtg gtgccatcga ccataaacag cacgcggtcg
      541 gcctgttcaa tttcctgcca cgcgcgctcg ataccaatac gttccacttc gtcactggct
      601 tcacgtagcc cagcggtatc gatgatatgc agcggcattc cgtcaatgtg gatatgctca
      661 cgcagcacgt cacgtgtggt tccggcgata tcagttacga ttgctgcttc acggcccgcc
      721 agcgcgttta acaggctcga tttaccggcg ttaggacgtc cggcaatcac caccttcatc
      781 ccttcgcgca acaaactacc ctgacgtgct tcagcacgca ctgcatcgag atcggcaatc
      841 acgtcattaa gctgggcttc aattttgccg tcggagagga aatcgatctc ttcatcaggg
      901 aaatcaattg ccgcttcgac gtaaatgcgc aagtgggtga gggcttccac cagatggttg
      961 acccgtgcgg agaatgcgcc ttgcagcgag ttaagtgccg aacgggccgc ctgttccgaa
     1021 ctggcgtcga taagatcggc aatcgcctcg gcctgggcta agtcgagttt atcgttaaga
     1081 aacgcgcgtt cggaaaactc accagggcga gcaatccgca ggccgggaat ggtcagaatg
     1141 cgttttaaca gcaggtcgag gatcaccgga ccgccatgac cttgcagttc cagcacatct
     1201 tcgccggtga acgagttcgg gccagggaac catagcgcaa tcccctgatc gagcacgctg
     1261 ccgtcggcgt ctttaaacgg aagataatcg gcgtagcgcg gcttaggtag tttacccagc
     1321 acggtttcgg caacttcacg ggctttgaag ccggagatgc gcaggatgcc aacgccgcca
     1381 cgtcccggag gcgtggcctg ggctacgata gtgtcattat cgctcatgat gttcctgttg
     1441 ctttgtgtgg cggatgcgcg gtgcttatcc gccctacgaa aagaaaaaag gcggtcaact
     1501 gaccgccctt attttagcga aaactcaccg aatcaggatt ttttcttctc gcggctatgc
     1561 aggccacgtt tttccagacc acggtaaatc agctgctgct gaataatggt taccaggttg
     1621 ctgacgatat agtacagcac cagacctgac gggaaccaca ggaagaacac ggtgaagatg
     1681 accggcataa aggtcatgat cttctgctgc atcgggtcgg tcactgtggt cggcgacatc
     1741 ttctgaatga agaacatcgt tacgcccatc aggatcggca agatgtagta cgggtcctgt
     1801 gccgacaggt cgtggatcca cagtgcaaac ggtgcctgac gcagttcaac ggaacccatc
     1861 agcatgtagt acaacgccag gaagattggc atctggatca gcagcgggaa gcagccgccc
     1921 agcgggttaa ccttctcagc tttgtacagc gccatcattt cctggctgat acgctgtttg
     1981 tcatcgccca gacgctcacg cattgcctga atcttcggct gcagcatacg catcttcgcc
     2041 atggaggtgt actgcgcttt ggtcagcggg tacatgatgc cacgaacgat aaaggtgatg
     2101 atgataatgg agaagcccca gttacccaca aagctatgga tccatttcag cagtttgaac
     2161 agcggctgag agatgaacca caaccaaccg taatcaacgg tcagatccag gtgcggagca
     2221 acagctgcca ttttgtcctg gatttccggg ccaacccaca gggtgctgtt catcgcgcca
     2281 gtctgaccag gctgaaccag taccggctga gatttatagc cgatagcggc gatgccgtta
     2341 cccagattag cggtatagaa gttgttggta ccgtcgttat gcgggatcca cgccgtcgcg
     2401 aaatactgtt gcagcatcgc cacccaacca cctttcgaag agatgttcag gttttcgtta
     2461 tcggcaatgg tatcgaactt gtatttctcg tacttctcgt caggcgtgga gtacgccgcg
     2521 ccgcggaagg tgtgcagtgc gaagttgctg cttccggtat cgagatgcgg tggcagagtg
     2581 atggattgct tcaactgacc aaaggtggag atttccagcg gtttctcgcc agcgttctgc
     2641 acgttgtagt tgacgttgac agcgtaatca ccacgtttca ggacaaacgt tttggtaaac
     2701 gtgttgcctg ccgcgtcggt atacgtcatc ggcacctgca gttcgttttg accttcagcc
     2761 agcacataag cgtctttttc aacgttatac agcggacgcg ggccgttagc cgggttatcc
     2821 gggccatcac gaccggtcag accgctctgt gcctgataaa taaactgcgg tgaagtttcc
     2881 agcagctgga acggctgggt agagttcagc tctttcgggt aagcaggcag cagagcttgc
     2941 tcaacatcac caccacgggt gttgatggtc agatcaagca cgtcggtctt aaccgagatc
     3001 agtttcccct ggccactggc cggtacgccc tggtcggcgg cgctacccgc tgcggtggtc
     3061 gttgtctgcg tggtctgttg ggcctgaggt tgcgggtttt tatcctgctc ccaggcttgc
     3121 cagatcatga aagacacgaa cagcaaagcg atgactaaaa gattgcgttg cgaatccatc
     3181 gttagtgttc tctggtatca aatggtccgg gcgggacggg atcgtcacca ccagggtgta
     3241 aagggtggca ttttaatacg cgtttcaccg tcaaccaact gccttttatc actccaaacc
     3301 tgcgcaatgc ctcaattccg tagcttgaac aggttggagt gaaacgacaa tgcggcccga
     3361 gtagcggact aatcaggcgt tgatagaccc gaatgagggc tatcaggacc cgcgagccag
     3421 gcgacagtgg cggcgccata atttttccaa cgcttccgag agagcacggt tatcgaggtc
     3481 ggcaacccct tttttcgcca ccaccacgaa atccatagcc gggagttcat gttggcgcag
     3541 acggaagctt tcacgcgtca gacgtttaat ccgattgcgt tcatgggcgc gtcgaacgtt
     3601 tttcttggcg actgtaagac cgatacgggg atgccccagc gaattcaggc ggccgagaat
     3661 ggtaatttgc ggcgtgccag cccgttgtgg ctgctggaag acgaatgtga attgactggg
     3721 agttaacaag cgtaactccc tgggaaatgc gagcttaacc actcaggggt tagctttatt
     3781 acttagaaac ggtcagacga gcgcggcctt tagcacgacg acgtgccaga acctgacgac
     3841 catttttagt agccatacga gcacggaagc cgtgagaacg gttgcgcttc agtacagacg
     3901 gttgaaaagt gcgtttcatg gcgatttcta cctaaacttg aataaattca atggctttat
     3961 tggatatccg ccgaaaaatg aaacgatgga caccgaagcc atgggtgatt aaagaggccg
     4021 gattgtaata attgtacact ccggagtcaa ttctctttcc ttatttaccg cgcttttccg
     4081 caccttttcg cagggaaaat gtacgacctc acaccagtgg aaaccagcat ggcgcgccgg
     4141 gtggaggatt atacgggctg atgggtaaag cgcaaggatc gtcctggatc tttattagat
     4201 cgattaagcc aatttttgtc tatggtcatt aaattttcca atatgcggcg taaatcgtgc
     4261 ccgcctcgcg gcaggatcgt ttacacttag cgagttctgg aaagtcctgt ggataaatcg
     4321 ggaaaatctg tgagaaacag aagatctctt gcgcagttta ggctatgatc cgcggtcccg
     4381 atcgttttgc aggatcttga tcgggcatat aaccgcagac agcggttcgt gcgtcaccct
     4441 caagcagggt cttttcgacg tacgtcaaca atcatgaatg tttcagcctt agtcattatc
     4501 gacttttgtt cgagtggagt ccgccgtgtc actttcgctt tggcagcagt gtcttgcccg
     4561 attgcaggat gagttaccag ccacagaatt cagtatgtgg atacgcccat tgcaggcgga
     4621 actgagcgat aacacgctgg ccctgtacgc gccaaaccgt tttgtcctcg attgggtacg
     4681 ggacaagtac cttaataata tcaatggact gctaaccagt ttctgcggag cggatgcccc
     4741 acagctgcgt tttgaagtcg gcaccaaacc ggtgacgcaa acgccacaag cggcagtgac
     4801 gagcaacgtc gcggcccctg cacaggtggc gcaaacgcag ccgcaacgtg ctgcgccttc
     4861 tacgcgctca ggttgggata acgtcccggc cccggcagaa ccgacctatc gttctaacgt
     4921 aaacgtcaaa cacacgtttg ataacttcgt tgaaggtaaa tctaaccaac tggcgcgcgc
     4981 ggcggctcgc caggtggcgg ataaccctgg cggtgcctat aacccgttgt tcctttatgg
     5041 cggcacgggt ctgggtaaaa ctcacctgct gcatgcggtg ggtaacggca ttatggcgcg
     5101 caagccgaat gccaaagtgg tttatatgca ctccgagcgc tttgttcagg acatggttaa
     5161 agccctgcaa aacaacgcga tcgaagagtt taaacgctac taccgttccg tagatgcact
     5221 gctgatcgac gatattcagt tttttgctaa taaagaacga tctcaggaag agtttttcca
     5281 taccttcaac gccctgctgg aaggtaatca acagatcatt ctcacctcgg atcgctatcc
     5341 gaaagagatc aacggcgttg aggatcgttt gaaatcccgc ttcggttggg gactgactgt
     5401 ggcgatcgaa ccgccagagc tggaaacccg tgtggcgatc ctgatgaaaa aggccgacga
     5461 aaacgacatt cgtttgccgg gcgaagtggc gttctttatc gccaagcgtc tacgatctaa
     5521 cgtacgtgag ctggaagggg cgctgaaccg cgtcattgcc aatgccaact ttaccggacg
     5581 ggcgatcacc atcgacttcg tgcgtgaggc gctgcgcgac ttgctggcat tgcaggaaaa
     5641 actggtcacc atcgacaata ttcagaagac ggtggcggag tactacaaga tcaaagtcgc
     5701 ggatctcctt tccaagcgtc gatcccgctc ggtggcgcgt ccgcgccaga tggcgatggc
     5761 gctggcgaaa gagctgacta accacagtct gccggagatt ggcgatgcgt ttggtggtcg
     5821 tgaccacacg acggtgcttc atgcctgccg taagatcgag cagttgcgtg aagagagcca
     5881 cgatatcaaa gaagattttt caaatttaat cagaacattg tcatcgtaaa cctatgaaat
     5941 ttaccgtaga acgtgagcat ttattaaaac cgctacaaca ggtgagcggt ccgttaggtg
     6001 gtcgtcctac gctaccgatt ctcggtaatc tgctgttaca ggttgctgac ggtacgttgt
     6061 cgctgaccgg tactgatctc gagatggaaa tggtggcacg tgttgcgctg gttcagccac
     6121 acgagccagg agcgacgacc gttccggcgc gcaaattctt tgatatctgc cgtggtctgc
     6181 ctgaaggcgc ggaaattgcc gtgcagctgg aaggtgaacg gatgctggta cgctccgggc
     6241 gtagccgttt ttcgctgtct accctgccag cggcggattt cccgaacctc gatgactggc
     6301 agagtgaagt cgaatttacc ctgccgcagg caacgatgaa gcgtctgatt gaagcgaccc
     6361 agttttcgat ggcgcatcag gacgttcgct attacttaaa tggtatgctg tttgaaaccg
     6421 aaggtgaaga actgcgcacc gtggcaaccg acggccaccg tctggcggtc tgttcaatgc
     6481 caattggtca atctttgcca agccattcgg tgatcgtacc gcgtaaaggc gtgattgaac
     6541 tgatgcgtat gctcgacggc ggcgacaatc cgctgcgcgt gcagattggc agcaacaata
     6601 ttcgcgccca cgttggcgac tttatcttca cctccaaact ggtggatggt cgcttcccgg
     6661 attaccgccg cgttctgccg aagaatccgg acaaacatct ggaagctggc tgcgatctgc
     6721 tcaagcaggc gtttgcccgt gcggcaattc tctctaacga gaaattccgc ggcgtgcgcc
     6781 tgtatgtcag cgaaaaccag ctgaaaatca ccgccaacaa cccggaacag gaagaagcgg
     6841 aagagatcct cgacgttacc tatagcggtg cggagatgga aatcggcttc aacgtcagct
     6901 atgtgctgga tgttctgaac gcgctgaaat gcgaaaacgt ccgcatgatg ctgaccgatt
     6961 cggtttccag cgtgcagatt gaagatgccg catcacagtc ggctgcctat gttgtcatgc
     7021 caatgagact gtaatgtccc tcacccgctt gttgatccgc gatttccgca acattgaaac
     7081 cgcggatctc gctttatctc ccggctttaa ctttctggta ggtgccaacg gcagtggcaa
     7141 aaccagcgtg ctggaagcca tctatacgct cggccatggt cgggcgtttc gcagtttgca
     7201 gattggtcgc gtcattcgcc atgagcagga ggcgtttgtt ctccacgggc gattacaggg
     7261 cgaagagcgc gagacagcga ttggcttaac caaagacaaa cagggcgaca gcaaagtccg
     7321 catcgacggt acagacgggc ataaggtcgc ggaactggcg cacctgatgc caatgcagtt
     7381 gataacgcca gaagggttta ctttactcaa cggcggcccc aaatacagaa gagcattcct
     7441 cgactgggga tgctttcaca acgaacccgg atttttcacc gcctggagca atctcaagcg
     7501 attgctcaag cagcgcaatg cggcgctgcg ccaggtgaca cgttacgaac agctacgccc
     7561 gtgggataaa gaactgatcc cgctggcgga gcaaatcagc acctggcgcg cggagtatag
     7621 cgccggtatc gcggctgata tggccgatac ttgtaagcaa tttctccctg agttttctct
     7681 gactttctct ttccagcgcg gctgggagaa agagacagaa tatgctgagg tgctggaacg
     7741 taattttgaa cgcgatcgcc agctaaccta caccgcgcat ggcccgcata aagcggactt
     7801 acgcattcgc gccgacggtg cgccggtgga agatacctta tcgcgtgggc agcttaagct
     7861 gttgatgtgc gccttacgtc tggcgcaagg agagttcctc acccgtgaaa gcgggcggcg
     7921 gtgtctctac ctgatagatg attttgcctc tgagcttgat gatgagcgtc gcgggctgct
     7981 tgccagccgc ttaaaagcga cgcaatcaca ggtctttgtc agcgcgatca gtgctgaaca
     8041 cgttatagac atgtcggacg aaaattcgaa gatgtttacc gtggaaaagg gtaaaataac
     8101 ggattaaccc aagtataaat gagcgagaaa cgttgatgtc gaattcttat gactcctcca
     8161 gtatcaaagt cctgaaaggg ctggatgcgg tgcgtaagcg cccgggtatg tatatcggcg
     8221 acacggatga cggcaccggt ctgcaccaca tggtattcga ggtggtagat aacgctatcg
     8281 acgaagcgct cgcgggtcac tgtaaagaaa ttatcgtcac cattcacgcc gataactctg
     8341 tctctgtaca ggatgacggg cgcggcattc cgaccggtat tcacccggaa gagggcgtat
     8401 cggcggcgga agtgatcatg accgttctgc acgcaggcgg taaatttgac gataactcct
     8461 ataaagtgtc cggcggtctg cacggcgttg gtgtttcggt agtaaacgcc ctgtcgcaaa
     8521 aactggagct ggttatccag cgcgagggta aaattcaccg tcagatctac gaacacggtg
     8581 taccgcaggc cccgctggcg gttaccggcg agactgaaaa aaccggcacc atggtgcgtt
     8641 tctggcccag cctcgaaacc ttcaccaatg tgaccgagtt cgaatatgaa attctggcga
     8701 aacgtctgcg tgagttgtcg ttcctcaact ccggcgtttc cattcgtctg cgcgacaagc
     8761 gcgacggcaa agaagaccac ttccactatg aaggcggcat caaggcgttc gttgaatatc
     8821 tgaacaagaa caaaacgccg atccacccga atatcttcta cttctccact gaaaaagacg
     8881 gtattggcgt cgaagtggcg ttgcagtgga acgatggctt ccaggaaaac atctactgct
     8941 ttaccaacaa cattccgcag cgtgacggcg gtactcacct ggcaggcttc cgtgcggcga
     9001 tgacccgtac cctgaacgcc tacatggaca aagaaggcta cagcaaaaaa gccaaagtca
     9061 gcgccaccgg tgacgatgcg cgtgaaggcc tgattgcggt cgtttccgtg aaagtgccgg
     9121 acccgaaatt ctcctcccag accaaagaca aactggtttc ttctgaggtg aaatcggcgg
     9181 ttgaacagca gatgaacgaa ctgctggcag aatacctgct ggaaaaccca accgacgcga
     9241 aaatcgtggt tggcaaaatc atcgatgctg cccgtgcccg tgaagctgcg cgtcgtgcgc
     9301 gtgaaatgac ccgccgtaaa ggtgcgctcg acttagcggg cctgccgggc aaactggcag
     9361 actgccagga acgcgatccg gcgctttccg aactgtacct ggtggaaggg gactccgcgg
     9421 gcggctctgc gaagcagggg cgtaaccgca agaaccaggc gattctgccg ctgaagggta
     9481 aaatcctcaa cgtcgagaaa gcgcgcttcg ataagatgct ctcttctcag gaagtggcga
     9541 cgcttatcac cgcgcttggc tgtggtatcg gtcgtgacga gtacaacccg gacaaactgc
     9601 gttatcacag catcatcatc atgaccgatg cggacgtcga cggctcgcac attcgtacgc
     9661 tgctgttgac cttcttctat cgtcagatgc cggaaatcgt tgaacgcggt cacgtctaca
     9721 tcgctcagcc gccgctgtac aaagtgaaga aaggcaagca ggaacagtac attaaagacg
     9781 acgaagcgat ggatcagtac cagatctcta tcgcgctgga tggcgcaacg ctgcacacca
     9841 acgccagcgc accggcattg gctggcgaag cgttagagaa attggtgtct gagtacaacg
     9901 cgacgcagaa aatgatcaac cgcatggagc gtcgttatcc gaaagcaatg ctgaaagagc
     9961 ttatctatca gccgacgctg acggaagccg acctctctga tgagcagacc gttacccgct
    10021 gggtgaacgc gctggtcagc gaactgaacg acaaagaaca gcacggcagc cagtggaagt
    10081 ttgatgtcca caccaatgcc gaacaaaacc tgtttgaacc gattgttcgc gtgcgtaccc
    10141 acggtgtgga tactgactat ccgctggatc acgagtttat caccggtggc gaatatcgtc
    10201 gtatctgcac gctgggtgag aaactgcgtg gcttgctgga agaagatgcg tttatcgaac
    10261 gtggcgagcg tcgtcagccg gtagccagct tcgagcaggc gctggactgg ctggtgaaag
    10321 agtcccgtcg cggcctctcc atccagcgtt ataaaggtct gggcgagatg aacccggaac
    10381 agctgtggga aaccaccatg gacccggaaa gccgtcgtat gctgcgcgtt accgttaaag
    10441 atgcgattgc tgccgaccag ttgttcacca cgctgatggg cgacgccgtt gaaccgcgcc
    10501 gtgcgtttat tgaagagaac gccctgaaag cggcgaatat cgatatttaa tggcgctaac
    10561 catgcgagcg tgcctgatgc gctacgctta tcaggcctac gaaaatcttg caatgtattg
    10621 aattttcatg attttgtagg ccggataagg cgttcacgcc gcatccggca agaagctata
    10681 agaaaagggc ggagatcatc tccgcccttt ttatttctgc aatccgcaat tatcatcccg
    10741 caaatctata ctttttgggt caacacgtaa cgctttacag gtgattatca tggggctttt
    10801 tgatgaagtt gtcggtgcct ttctgaaagg cgatgcgggg aaatatcagg ctattttaag
    10861 ttgggttgag gagcagggcg gcattcaggt gctgctggaa aaactgcaaa gtggcggctt
    10921 aggggccatt ctctcaacct ggctgagtaa tcaacagggc aatcaatcgg ttagtggcga
    10981 gcaactggaa tcggcgctcg gcacaaatgc ggtgtccgat ctcgggcaaa aacttggcgt
    11041 ggataccagt acagcttcca gtttactggc agagcaattg ccgaagatta ttgatgcgct
    11101 ctcaccgcaa ggtgaagtgt caccacaagc caataacgat ctgctttccg caggtatgga
    11161 actgctgaaa gggaaactct tccgctaagc aaatggggag catgcaaccc gcgttgcatc
    11221 ccccttttcc ctcgggtgtt gtactgattt ttgagcggaa tcgcgttagc atgggtcagg
    11281 aaccaatcta cctggggaac tcatggctat caaactcatt gctatcgata tggatggcac
    11341 ccttctgctg cccgatcaca ccatttcacc cgccgttaaa aatgcgattg ccgcagctcg
    11401 cgcccgtggc gtgaatgtcg tgctaacgac gggtcgcccg tatgcaggtg tgcacaacta
    11461 cctgaaagag ctgcatatgg aacagccggg cgactactgc attacttata acggcgcgct
    11521 ggtacagaag gccgctgatg gtagcaccgt ggcgcaaact gctctcagct atgacgacta
    11581 tcgtttcctg gaaaaactct ctcgcgaagt cggttctcat ttccacgccc tggaccgcac
    11641 cacgctgtac accgccaacc gtgatatcag ctactacacg gtgcatgaat ccttcgttgc
    11701 caccattccg ctggtgttct gcgaagcgga gaaaatggac cccaataccc agttcctgaa
    11761 agtgatgatg attgatgaac ccgccatcct cgaccaggct atcgcgcgta ttccgcagga
    11821 agtgaaagag aaatataccg tgctgaaaag tgcgccgtac ttcctcgaaa tcctcgataa
    11881 acgcgttaac aaaggtacgg gggtgaaatc actggccgac gtgttaggta ttaaaccgga
    11941 agaaatcatg gcgattggcg atcaggaaaa cgatatcgca atgattgaat atgcaggcgt
    12001 cggtgtggcg atggataacg ctattccttc ggtgaaagaa gtggcgaact ttgtcaccaa
    12061 atccaacctt gaagatggcg tggcgtttgc tattgagaag tatgtgctga attaatcagt
    12121 ggacgggcaa acagcataaa cgcgtttgcc cgcttactga ttattggttt actgcatata
    12181 caaccagaaa ttgcagcggt gcagtaatga tgtccagcgt aacagtcact ggcagtaatg
    12241 ctgcggaaac accatccgga atgcctctgg gtgaatagta ttcataaaat cgcactttaa
    12301 acggatgata gaacgccatc acctttgagt aatccatgtt tttatttttc tcatggattg
    12361 tgccttttag gttttccagt gacctggtac agtgttgaac accacaggca aaaccataat
    12421 gcgttgccag tgccttttct tcttcgttat tccagtagta gtaaagcgat attgtgccgg
    12481 taaatttctt tttccccgca tttaaaacaa atcttgcgtc atcggcaacc tgaatattgt
    12541 gccggttaag tgctggatca ttgagcatct taacgatgtc atcggcacca tcagtgataa
    12601 ggtaatccag cgattcccct acaaaaacat agccttttgt tccattacta tcttttgcct
    12661 gcgatagccc agtgatggtg tctgactgcg attgatactt tcctgaacta ccctttgcgg
    12721 tatgggaaac cagagcatac gagatacagc ctgaaagcat cagcgccagt gggaataaac
    12781 cggcagcctt aaaaaatccc ttaaaattca acttcatgat taatccttta aacgagtagc
    12841 taagccgttg aaaacatact cttctttccc tcctgtttag cgcctgatat cccttttcag
    12901 atttctgccc gacgcatgtc atttttttat gcattgttct ttttgtgatc taaattgtag
    12961 tacaacaata taagtttgta ctacattacg cacacggcaa acgcgaacgt catcacgcta
    13021 gtactacaaa gttgccgcgt tatgcatcga tcggggtaaa gtagagaaga acatacagag
    13081 cacaaggact ctccatgact ctcaataaaa ccgatcgcat tgtcattacg ctgggtaaac
    13141 agatcgttca cggcaaatac gtacctggct cgccacttcc ggctgaggcg gagctctgtg
    13201 aagagtttgc aacctcgcgc aacatcatcc gtgaggtgtt ccgttcgttg atggcgaagc
    13261 ggctgattga aatgaaacgt tatcgcggcg cgtttgtggc accgcgtaac cagtggaatt
    13321 acctcgacac tgacgtactg caatgggtgc tggaaaacga ctacgaccca cggcttatca
    13381 gtgccatgag cgaagtgcga aatctggtgg aaccggcgat tgcccgttgg gcagcagagc
    13441 gcgcgacctc cagcgatctg gcgcagattg aatcggcgct gaacgagatg attgccaaca
    13501 atcaggaccg cgaagcgttt aacgaagcgg atattcgcta ccacgaggcg gtgctgcagt
    13561 cggtgcataa cccggtgtta cagcaactta gcattgcgat cagttcgctg cagcgggcgg
    13621 tttttgaacg aacctggatg ggcgatgagg ccaacatgcc gcaaacgctc caggaacata
    13681 aggcgctgtt cgatgcgata cgacatcagg acggcgatgc ggcagagcag gcggcgctta
    13741 ccatgatcgc cagctcgaca cgaaggttaa aggaaatcac atgacagctc gctacatcgc
    13801 aattgactgg ggatcgacca atctgcgtgc ctggctttat cagggcgacc actgcctgga
    13861 cagcaggcaa tcagaagcag gcgtcacgcg cctgaacgga aaatctccgg ctgcggtgtt
    13921 agcagaagtc acgaccgact ggcgtgaaga gaatacgcca gtggtgatgg caggaatggt
    13981 cggcagcaat gtcggctgga aagttgcacc gtatttatct gttcctgccc gtttttcgtc
    14041 tattggcgat caattaacgt cggttggcga caatatctgg attattcccg gattatgcgt
    14101 ctctcatgac gataaccaca atgtgatgcg cggcgaagaa acacaattga tcggcgcgcg
    14161 cactctggct ccttcctctc tttatgtcat gcccggaacc cattgcaaat gggtgcaggc
    14221 cgatagccag caaatcaacg attttcgcac cgtgatgacc ggtgaattac atcatttact
    14281 gttaaatcac tcattgattg gcgcaggttt gccgccgcag gaaaactctg ccgatgcctt
    14341 cgcggctggc cttgagcgtg gccttaatgc gcccgccata ttgccgcagc tttttgaagt
    14401 tcgcgcctcg catgtgctgg gaacacttcc ccgcgaacag gtcagcgaat ttctctctgg
    14461 tttgttgatt ggcgcagagg tcgccagtat gcgcgactat gtggcccatc aacacgccat
    14521 cacccttgtc gccggaacat cgctgaccgc gcgctaccag caagcctttc aggcgatggg
    14581 ttgcgacgtg acagcggtag caggcgacac ggcatttcag gctggtataa ggagcatcgc
    14641 tcatgcagtg gcaaactaaa ctcccgctga tcgccatttt gcgcggcatt aagcccgacg
    14701 aggcgctggc gcatgttggc gcggtgattg acgccgggtt cgacgcggtt gaaatcccgc
    14761 tgaattcccc acaatgggag caaagcattc ccgccatcgt tgatgcgtat ggcgacaagg
    14821 cgttgattgg cgcaggtacg gtactgaaac ctgaacaggt cgatgcgctc gccaggatgg
    14881 gctgtcagct catcgttacg cccaatatcc atagtgaagt gatccgccgt gcggtgggct
    14941 acggcatgac cgtctgcccc ggctgcgcga cggcgaccga agcctttacc gcgctcgaag
    15001 cgggcgcgca ggcgctgaaa atatttccgt catcggcttt tggtccgcaa tacatcaaag
    15061 cgttaaaagc ggtattgcca tcggacatcg cagtctttgc cgttggcggc gtgacgccag
    15121 aaaacctggc gcagtggata gacgcaggtt gtgctggggc gggcttaggc agcgatctct
    15181 atcgcgccgg gcagtccgta gaacgcaccg cgcagcaggc agcagcattt gttaaggcgt
    15241 atcgagaggc agtgcaatga aaatcaccaa aattaccacg tatcgtttac ctccccgctg
    15301 gatgttcctg aaaattgaaa ccgatgaagg cgtggtcggt tggggcgagc ccgtgattga
    15361 aggccgcgcc cgtacggtgg aagcggcagt tcacgagctg ggtgactatt tgattggtca
    15421 ggatccttcg cgcatcaatg acttatggca agtgatgtat cgcgccggat tttatcgtgg
    15481 cggtccaatc ctgatgagcg ccattgccgg gatcgaccag gcgttatggg atatcaaagg
    15541 taaagtgctg aatgcgccgg tctggcaact gatgggcggc ctggttcgcg acaaaattaa
    15601 agcctacagt tgggtcggcg gcgatcgtcc ggcggatgtg atcgacggta ttaaaaccct
    15661 gcgcgaaatc ggcttcgata ccttcaaact gaacggttgt gaagaactgg ggctaattga
    15721 taactcccgc gcggtagatg cggcagtcaa caccgtggca caaattcgtg aagcttttgg
    15781 caatcagatt gagtttggtc ttgatttcca cggtcgcgtc agcgcgccaa tggcgaaagt
    15841 gctgattaaa gaactggagc cgtatcgccc gctgtttatt gaagagccgg tgctggcgga
    15901 acaagccgaa tactacccga aattggcggc acaaacgcat attccactgg cggcgggtga
    15961 gcgcatgttc tcacgtttcg attttaaacg cgtgctggag gcaggcggta tttcgattct
    16021 acaaccggat ctctcccatg caggcggtat taccgaatgc tacaaaattg ccggaatggc
    16081 agaagcctat gacgtgaccc ttgcgccgca ctgtccgctc ggaccgattg cactggcggc
    16141 ttgtctgcat atcgactttg tttcctataa cgcggtactt caggaacaaa gtatgggaat
    16201 tcattacaac aaaggcgcgg agttactcga ctttgtgaaa aacaaagaag acttcagcat
    16261 ggttggcggc ttctttaaac cgctaacgaa accgggctta ggcgtggaaa tcgacgaagc
    16321 taaagttatt gagttcagta aaaatgcccc ggactggcgt aatccgctct ggcgtcatgt
    16381 agataacagc gtagcagagt ggtaattcct gccacgtaag cccctcatcg ggcactaaaa
    16441 cagcaataca aaaatataac cctctgtaaa ttacagggca tggtgagcgg cttcgctatg
    16501 cccaaaatct ggataaagct cgctatggat attcccgtta atgcagcaaa gccggggcgt
    16561 cggcgttatc tgacgctggt gatgatcttt attacggtgg tcatttgtta tgttgaccgc
    16621 gctaacctgg ccgtggcttc cgcccatatt caggaagagt tcggcattac gaaagcggaa
    16681 atgggctatg tattttcggc cttcgcctgg ctttatacgc tatgccagat ccccggcggt
    16741 tggtttttag atcgcgtagg ttctcgcgtg acttattata ttgcgatatt tggttggtca
    16801 gtggcgactt tattccaggg ctttgccacg ggattaatgt cattaattgg tctgcgtgcg
    16861 ataaccggta ttttcgaagc gcccgctttc ccgaccaata accggatggt gaccagctgg
    16921 ttcccggaac atgaacgcgc ttccgccgtt ggtttttata cgtctggtca gtttgtcggt
    16981 ctggcgtttt tgactccgct gctgatctgg attcaggaga tgttgagctg gcactgggtg
    17041 ttcattgtca ctggtggtat cggcattatc tggtcgctga tttggtttaa ggtttatcag
    17101 ccgccgcgcc tgaccaaagg tatcagcaaa gctgaactgg attacattcg tgatggcggc
    17161 ggtctggtgg atggtgatgc gccggtgaag aaagaggcgc gtcagccgtt aacagccaaa
    17221 gactggaaac tggtgttcca tcgtaaactg atcggcgttt atcttgggca atttgcggtg
    17281 gcttctacac tgtggttttt cttaacctgg ttcccgaact atttaaccca ggaaaaagga
    17341 atcacggcgc tgaaagcagg ctttatgacc acggtgccat tcctcgcggc gtttgtcggc
    17401 gtcctgctct ctggctgggt agcggatctg ctggtacgta agggcttttc actgggcttt
    17461 gcgcgtaaaa cgccgattat ctgcggcttg ctgatctcca cctgcattat gggcgctaac
    17521 tacactaacg atccgatgat gattatgtgc ctgatggcgc tggcattctt cggtaacggt
    17581 tttgcttcga ttacctggtc gctggtttct tctctggcac cgatgcgcct gattggttta
    17641 accggcggcg tgtttaactt cgccggtggt ctgggcggca tcaccgttcc gctggtggtg
    17701 gggtacctgg cgcagggtta cggtttcgca cctgcactgg tttatatctc cgccgtcgcg
    17761 ttgattggcg cgctctctta tatcctgctg gtgggcgatg tgaagcgcgt tggctaatcc
    17821 ttcaactgtg gaatatgtgc cacaccactg cgcataatcc actttcttaa agccggtgcg
    17881 gtcaggcatc ggctttttac tatcttgccg aagagtttta aacgcagttt gcgggtggcg
    17941 cgccagtaag cggtattgag cttctctggc tgcttcagta acaccgaacg cagaatgtct
    18001 gcgctatcca gcgcatagct aatcccctcc agcgagctgg cgctgataaa tcccgccgct
    18061 tcaccaatca gaaaggcgtt gtccttaccg cagacaaaat cctgccagcg tgagggaaac
    18121 agcaccgtgc atttttcgct tttcaccgcc ttaccaaact ggaactgaaa ggcgctcatt
    18181 ttctctttca gcgtcgtgaa acgcgtctga ccgtctttca ttggataagc accgccaaag
    18241 ataaaataac cgtctttgct gatactccat gaataacagt tagttatcga attatcaaag
    18301 atgcaggagt agaacggcac cggatgtttc tccgcgaacc actgctggat agcgacatat
    18361 ttacggattt gatgatccgg gtagagatgt cgccgcacca tcgagtttgc gccatcggca
    18421 ccgaccagat agcgggcggt aatatgttgc tcccagccgt ctgcacgaaa aatgacatgc
    18481 catttatcat cctcacgcca gatttttcgg cacaggctat cgtgataaac ctcaacgctg
    18541 gtggggatca gcgatttcat ccacaagtcg aaagcatggc gattaatatt gatatagctt
    18601 cgctggtagt tacgcgtgag cgatgcggcg acgtcgacag ttttgacgct gaaaatctgt
    18661 ggattggcga tcacatcgac aggaagcgtc agtccatcgc gaataaaaga tcgctgcgcg
    18721 tccggtgcca gcagaccgcc gcaaggtttg ctgaaacctt cagtaccaca ctggtgcttt
    18781 ttatccagcg cgatcacctg cattttgcct gctaattttc gcgccaacgc cgatcctgcc
    18841 gggccgaggc caataatcgc cacgtcgaaa tgttccatct tttcactcac attcatcacg
    18901 atggcaaaag actattgtct gaaagtgaag tccttgtgaa gtatgatggc gggacccgtt
    18961 ttgtatcagg accgcgccat gaaacagata acctttgctc cccgtaatca cctgctcacc
    19021 aataccaata cctggacgcc cgacagccag tggctggtat ttgacgtgcg tccttctggg
    19081 gcgtcgttta ccggcgagac cattgagcgt gtgaatatcc ataccggcga ggtcgaggtt
    19141 atctatcgcg cgtcacaggg cgcacacgtc ggcgtggtga ccgttcatcc gaagtcagag
    19201 aaatatgttt tcatccatgg cccggaaaat cctgatgaaa catggcatta cgatttccat
    19261 catcgacgcg gagtcattgc tgaaggcggc aaggtgagca atctcgatgc aatggatatt
    19321 accgcgccgt atacgccagg agcgctgcgc ggcggcagcc atgtgcatgt ctttagcccg
    19381 aacggtgaaa gggtgagctt tacctataac gaccatgtaa tgcatgaact cgatccggcg
    19441 ctggatttgc gaaacgtcgg tgttgctgcg ccgtttggcc cggtcaacgt acaaaagcag
    19501 catccgcgtg aatacagcgg tagccactgg tgcgtgctgg tgagtaaaac cacgcccacg
    19561 ccgcagcctg gcagcgatga aatcaatcgt gcttatgaag aaggatgggt aggaaatcac
    19621 gcgctggcgt ttattggcga cacactttcg ccaaagggcg agaaagtgcc ggagctgttt
    19681 atcgttgagt taccgcaaga tgaagctggc tggaaagcgg caggtgatgc gccgttaagt
    19741 ggaacggaaa ccaccctgcc cgcgccaccg cgcggcgtcg tgcagcgacg tttaaccttt
    19801 acccaccatc gggcttatcc ggggttagtc aacgtcccgc gccactgggt gcgctgtaat
    19861 ccgcagggta cgcaaatcgc gtttttaatg cgtgatgata acggcattgt gcaactgtgg
    19921 cttatctcgc cacagggcgg cgagccgcgc cagttaaccc ataacaaaac ggatattcag
    19981 tctgcattta actggcatcc gtcaggagaa tggttgggct ttgtgctgga taatcgaatt
    20041 gcttgtgccc atgcgcaaag cggcgaggtt gagtatttaa ccgaaaacca cgccaatcca
    20101 ccttctgcgg acgccgtggt cttctcgccg gatggtcagt ggctggcgtg gatggaaggc
    20161 ggccagctgt ggatcaccga aactgatcgc taaatcagtt atcaggcatt ctggccggtg
    20221 gaataaccgc attggtggct tgcgcgttgg cttcgctttt ttcaacgcgt gagcgcaccg
    20281 aactgtcttt gcggaacaca tcccacggca gtaaaagcgt gtccatcaca gcggtaaacg
    20341 gcatatcgag aatcgccagc gacttagtgc cccagttggt ttcatcgtcg ccaatcatgg
    20401 ttgcgctggc gcgggtgccg ggatacgttc cttctttccc tccggtatgg gacatcacgc
    20461 tggagcatcc tccgagtaat gtcattccgc tgcatatcat gaacgctaac aacacatttc
    20521 ttatcataat aaattcatct gttgatcgtg ggtgttggcc tgatgagtta tagcgatccc
    20581 ttgctgaaaa taacatcatc attacgtcgc actgtggcgg ctatcgcact ttaacgtttc
    20641 gtgctgcccc ctcagtctat gcaatagacc ataaactgca aaaaaagtcc gctgataagg
    20701 cttgaaaagt tcatttccag acccattttt acatcgtagc cgatgaggac gcgcctgatg
    20761 ggtgttctgg ctacctgacc tgtccattgt ggaaggtctt acattctcgc tgatttcagg
    20821 agctattgat tatgcgtaac tttgatttat ccccgcttta ccgttctgct attggatttg
    20881 accgtttgtt taaccactta gaaaacaacc agagccagag taatggcggc taccctccgt
    20941 ataacgttga actggtagac gaaaaccatt accgcattgc tatcgctgtg gctggttttg
    21001 ctgagagcga actggaaatt accgcccagg ataatctgct ggtggtgaaa ggtgctcacg
    21061 ccgacgaaca aaaagagcgc acctatctgt accagggcat cgctgaacgc aactttgaac
    21121 gcaaattcca gttagctgag aacattcatg ttcgtggcgc taacctggta aatggtttgc
    21181 tgtatatcga tctcgaacgc gtgattccgg aagcgaaaaa accgcgccgt atcgaaatca
    21241 actaattcct taaggccgcc tggcgcggcc tgacatctcc atgctcgccg tcagggagca
    21301 tatgcgaatc ttcggatttg caggtactta ctcgcttctt agaaggagaa atgactatgc
    21361 gtaacttcga tttatcccca ctgatgcgtc aatggatcgg ttttgacaaa ctggccaacg
    21421 cactgcaaaa cgccggtgaa agccagagct tcccgccgta caacattgag aaaagcgacg
    21481 ataaccacta ccgcattacc cttgcgctgg caggtttccg tcaggaagat ttagagattc
    21541 aactggaagg tacgcgcctg agcgtaaaag gcacgccgga gcagccaaaa gaagagaaaa
    21601 aatggctgca tcaagggctt atgaatcagc catttagcct gagctttacg ctggctgaaa
    21661 atatggaagt ctctggcgca accttcgtaa acggtttact gcatattgat ttaattcgta
    21721 atgagcctga acccatcgca gcgcagcgta tcgctatcag cgaatgtccc gcgttaaata
    21781 gctaactagc tattctcttt gccccgccat tctggtgggg ctttttttgt ctttcttctt
    21841 caacaatgcg ttaaatttcg cctgtttaag atatttcggc acgttctttc ccgtgataat
    21901 gtgcgctata aggcaaatct gcttcaccga ttcagccata atccgtagca attaaaaatg
    21961 ttatctcaag agaaatggac gatgagtgat atagcattaa cggtcagtat tctggctttg
    22021 gtggcagtcg tcggtttgtt tatcggcaat gtcaaatttc gcggcatagg attaggtatt
    22081 ggcggcgtgc tgtttggtgg gatcatcgtc ggccattttg tttctcaggc ggggatgaca
    22141 ttaagtagcg atatgctgca tgttattcag gaatttggcc tgatcctgtt cgtttatact
    22201 atcgggattc aggtagggcc gggcttcttt gcctcattgc gcgtctccgg attacgcctc
    22261 aacctgtttg ctgttctgat cgtcatcatc ggtggtctgg ttaccgccat cctgcataaa
    22321 ctgtttgata ttccactgcc ggtagtgctg gggattttct ccggtgcggt taccaatacg
    22381 ccagcgctgg gggcagggca gcagattttg cgcgacctgg gtacaccaat ggaaatggtc
    22441 gatcagatgg ggatgagtta cgcgatggcg tatccattcg gcatttgcgg gattttgttc
    22501 accatgtgga tgttgcgggt tattttccgc gtcaatgtcg agacagaagc tcagcagcac
    22561 gagtcttcac gcaccaatgg cggcgcgctg atcaagacta tcaatattcg cgttgagaac
    22621 cctaacctgc atgatttagc cattaaagat gtaccgattc tcaacggcga caaaattatc
    22681 tgctcgcgtc tgaaacgcga agaaacccta aaagttcctt cgccagatac cattattcaa
    22741 ctgggcgatt tgctgcatct ggtgggtcag ccagcggatt tacataatgc gcaactggtg
    22801 attggtcagg aggtcgatac ttcgctgtcc acgaaaggca ctgatttgcg cgtcgagcgt
    22861 gtggtggtca ccaatgaaaa cgtgctcgga aaacgtattc gcgacctgca ctttaaagaa
    22921 cgctatgacg ttgttatctc gcgcctgaac cgtgccgggg tcgaactggt cgccagtggc
    22981 gatatcagcc tgcagttcgg cgatatcctc aacctggtgg ggcgtccgtc cgcaattgat
    23041 gccgttgcca atgtgctggg gaatgcgcag caaaaactgc aacaggttca gatgctgcca
    23101 gtgtttattg gcatcgggct tggcgtattg ttaggttcta ttcccgtctt tgtgccagga
    23161 ttcccggccg cgttgaaact ggggctggcg ggcggtccgc tgattatggc gttgatcctc
    23221 gggcgtatcg gcagtatcgg caagctgtac tggtttatgc cgccaagtgc caacctcgcg
    23281 ctgcgggagc tggggatcgt gctgttcctc tcggtcgttg gtctgaaatc tggtggggat
    23341 tttgtgaata ccctggtcaa tggcgaaggg ctaagctgga ttggttatgg tgccctgatc
    23401 accgccgttc cgctgattac tgttggcatt ctggcgcgga tgttagccaa aatgaattac
    23461 ctgaccatgt gcgggatgct ggcaggttcc atgaccgatc ctccggcgct ggcgtttgct
    23521 aataatcttc atccaaccag tggtgcggcg gcgctctctt acgccactgt ctatccgtta
    23581 gtgatgtttc tgcgaattat caccccccaa ttactggcgg tgctcttctg gagtatcggt
    23641 taactctttt ccgggtggag tcgccgtaaa tggtactcca cctggtattc gctggcattt
    23701 ctgaacatca ctgaataatt caaaaactcc ccgctctcgc tatatgacaa cgaggtgatc
    23761 cgcagcagtg gcgtgtgttc cgctaccttc atttgccgcg ccagcagtct atcggcgagc
    23821 accggcgtca ggctttcata attaccgcca atcaaaatcc cgcactcttg ttcaatatat
    23881 tcaaacttcg acccttccag atgttgcagc gaaaggttac ggaacagttt taccggcata
    23941 tagctgtctt ccagcatcag cggtttccct tccacaaaac gaacgcgacg ggagaagtag
    24001 atctgctcgt tgatttgaat ccgtaactgg ctggcaatga ccggaggcgc aggcatgatt
    24061 tcaaaaatca gcacctggct ggtgaccgtt tttccctgcc gttttaacac ctccaccagc
    24121 ccggtcaggc tggcggtttg atgcagtaca tctttgcgca ccaggtaagt gccgctgccg
    24181 tggcggcgga ccaccaatcc ccacgctacc agcaggtcaa tggctttacg gatggtcatc
    24241 cgcgataccg caaattcttc cgccaacttt ttttcaccgg gaagaaggct atttagcgtg
    24301 aaatctgcgg aattaagtcg aattcttaac cgctccgcaa tgcttttgta gatcatctct
    24361 agatctcttt tttgcctgca agcccgggta atgcgcccac aagttctgct taatcgattg
    24421 aaaaaacatc ttttttttaa agatgtgttc gatgctgtgt acctgtgctc acagatgtct
    24481 actttttcgc gaaaacgtag atctctaccg cccaacgaaa agcatgaaag cgatcacgaa
    24541 tcccattggg tcgtcatgtt ctgccgatcg catcttccta tcctcgctcc aggcctgccg
    24601 cataaccaat caggcttcct acttacagaa ttgagaaaag aggatgtgga aatgctcagt
    24661 caaattcaac gctttggcgg cgcgatgttc acgccagtgc tgctgtttcc cttcgccggg
    24721 attgtggtgg gtcttgccat cttgctgcaa aacccgatgt ttgtcgggga atcactgacc
    24781 gatccgaaca gtttattcgc gcaaatcgta cacattattg aagagggcgg ttggacggta
    24841 ttccgtaata tgccgctgat ttttgctgtc ggtttaccca ttggccttgc taagcaagcg
    24901 cagggacgtg cttgtctggc ggtgatggtg agtttcctga cctggaacta tttcatcaat
    24961 gcgatgggaa tgacctgggg aagctacttc ggcgtcgatt tcactcagga cgcggtggca
    25021 ggtagcggtc tgacaatgat ggctgggatt aaaaccctcg ataccagcat tatcggcgca
    25081 attatcattt ccggcattgt gacggcgctg cataaccgtc tgttcgataa aaaactgccg
    25141 gtgtttctcg gcattttcca ggggacgtct tatgtggtga ttatcgcctt cctggtgatg
    25201 atcccctgtg cctggctgac attgctcggc tggccaaaag tacaaatggg gattgaatct
    25261 ctgcaagcgt tcctgcgttc ggcgggtgcg cttggggtgt gggtttacac cttcctcgaa
    25321 cgtattctga tcccaaccgg tttacaccac ttcatctacg gaccgtttat ctttggtccg
    25381 gcagctgttg aaggcggcat tcagatgtac tgggcgcagc atctgcaaga gttcagtctg
    25441 agcgccgagc cgctgaaatc gttgttcccg gaaggcggtt ttgccctgca cggtaactca
    25501 aaaatctttg gtgccgtggg catttcttta gcgatgtact tcactgccgc accggaaaat
    25561 cgggtaaaag tggcgggctt gctgattccc gcaaccttaa ccgccatgct ggtgggaatt
    25621 accgaaccgc tggaatttac cttcctgttc atttcaccgt tgctgtttgc ggtacacgcc
    25681 gtgctggcgg cctcaatgtc gaccgtaatg tatctctttg gtgtggtggg caacatgggc
    25741 ggaggtctga ttgaccaggt tttaccgcaa aactggatcc cgatgttcag caaccacgcg
    25801 gatatgatgc tgacccaaat cgccattggg ttgtgcttta ccctgctgta cttcgtggtt
    25861 ttccgcacac tgattctgca gttcaacatg tgcacgccgg gacgtgaaga tgcggaagtg
    25921 aaactctact caaaagccga atacaaagcc tcgcgaggcc aaaccaccgc ggcagagcca
    25981 aaaaaagagc tggatcaggc tgccggtatc ctgcaagccc tgggcggggt cggcaatatc
    26041 tccagcatta acaattgcgc gacgcgttta cgcattgcac tgcatgacat gtcacaaacg
    26101 ctggatgacg aagcctttaa aaagctggga gcgcacggcg tcttccgtag tggcgatgcc
    26161 attcaggtga tcattggtct gcatgtatcc cagctgcgtg aacagctcga tagcttaatt
    26221 aattctcatc aatcagcaga aaatgttgcc attacggagg cagtataatg accaaattct
    26281 cagtggttgt cgcaggcggt ggaagcacct ttacgccagg catcgtgttg atgctcctgg
    26341 cgaatcagga gcgtttcccg cttcgtgcac tgaaatttta tgataacgat ggcgcgcggc
    26401 aggaagtgat tgccgaagcc tgtaaagtca tccttaaaga gaaagcgccg gacattgcgt
    26461 ttagttacac caccgatcct gaagtggcgt tcagcgacgt tgattttgtc atggcgcaca
    26521 tccgcgtcgg caagtacccg atgcgcgaac tggatgaaaa aatcccactg cgccacggtg
    26581 ttgttggtca ggaaacttgc ggacccggcg gaatagcgta cggcatgcgt tccattggcg
    26641 gcgtcctgga actggtggat tatatggaaa aatattcacc aaatgcctgg atgctcaact
    26701 actccaaccc ggcagccatt gtcgcagaag ccacgcgtcg tctgcgcccg aatgcgaaaa
    26761 tcctcaacat ctgtgacatg ccaatcggta ttgaaagccg gatggcgcaa attgttgggc
    26821 tgcaagatcg caaacagatg cgcgtgcgct actacggcct gaaccactgg tggtcggcga
    26881 tatcccgcag tttcagaaag ggttaatgag tcagcaagtg gcggtggaaa aactggtcgt
    26941 ggacgcctgg gaacagcgtt catatcagca cctgtggcag gcgattacgt tgtcgaaaac
    27001 ggtaccgagc gcctcggtcg ccaaagctat tctggatgag ttgctggagg ccaacaaagc
    27061 gtactggcca gagttacgtt aattgtgtgg actggcatcc gttgggtgct ggtccgtatt
    27121 ctttagcacg gctgacggcg cataaccaaa tttatgttta aaggctttgc tgaaatgaaa
    27181 ggagtcgaag aagttgagcg tatcagcgac ttcgccaacc gagtttcctt gttgctttaa
    27241 tagcgatagc gccagatcca gacgcgcatc gaggtaatat tctttcggcg tcttgccggt
    27301 atagcgtaaa aacagacgac gcaaccaggc ttcgctacag gggatcgtgg cagccatatc
    27361 agctacgctc cagcgttgtt gcagactggc atgtaaagtg gcaattaatt tttcaatttg
    27421 ccgccgttgt ggatcttttt taccatctgc gtacataaga caaatccact gataaataat
    27481 tttcgtcaga aaagcgactg ccagattatt ttttattgct tctggtgaag ttattaactc
    27541 agcaacttcg gtgagttcct gattataaat ttcgccatta taaataacgc tttgctgacc
    27601 aacgggaata tccatcatac tggtgggggt aaattccatc cagtactgtt cccagactaa
    27661 accttcacag tgataagagt gaatatccat tggctttaag aatataatac agttgccatg
    27721 cagtgttatt tgttcgccat tctttaaaag aattttccca catccttgta cggtatacac
    27781 cgcaacccat gagttggcga taagcggttt cttcttgtcc cgcggccaga tcacccggta
    27841 atgctcgtcg gcgtaggtat gccagagcga gatcagagaa attccgctgg agatcacatt
    27901 ttcgttaatc aataagggaa tattgtacgg agtttcgttt tttacatctg aactttgcaa
    27961 ttttccattc attttaatat ccctccggcg aattatgctc acaacctgaa cttaacagaa
    28021 aacaatccgt tcagggatac gacgtttgta ttttaagaat ctgactgcct gacccgacgc
    28081 attttacctc tccctatatt catgcgtccg ggacataaac ataaataagg gctatgagat
    28141 gaattcgtta caaatcttga gttttgtcgg ttttacgctg ctggtggcgg tgatcacctg
    28201 gtggaaggtc cgcaaaacag ataccggatc gcaacaaggc tattttcttg ccggacgttc
    28261 actaaaagcg ccggttattg ccgcttcgtt aatgctaacc aacctttcca cggaacaact
    28321 ggtcggcctt tccgggcagg cctacaaaag cggcatgtcg gtgatgggct gggaagtgac
    28381 ttcagcggtg acgctgatct tcctcgcgct aatcttttta ccgcgctatc tgaagcgcgg
    28441 cattgccacc atccccgatt ttctggagga acgttatgat aaaacgacgc gtattatcat
    28501 cgacttctgc ttcctcattg ccaccggcgt ctgctttctg ccgattgttc tctactccgg
    28561 cgcgttggcg ctcaacagcc tgtttcacgt cggggaatcg ctacagattt ctcacggtgc
    28621 ggctatctgg ctactggtaa ttttgcttgg tctggcggga attttgtatg cggtgatcgg
    28681 cggactgcgc gcaatggcag tggcggactc catcaacggt attgggctgg ttatcggcgg
    28741 gttgatggtg ccggtatttg gcctgatcgc gatgggcaag ggcagcttta tgcagggcat
    28801 tgagcaactc accaccgttc acgccgagaa attaaactca atcggtggcc cgaccgatcc
    28861 cttgccgatt ggcgcggcat ttaccggttt gattctggtg aacacctttt actggtgtac
    28921 aaatcagggc atcgtgcaac gcacgctggc gtcaaaaagc ctggcggaag ggcaaaaggg
    28981 ggcgctgtta acggcggtgc tgaaaatgct cgacccgctg gtactggtgc tgccagggtt
    29041 gattgcgttt catctgtatc aggatttacc gaaagccgac atggcctacc cgacgctggt
    29101 caataacgtt ctgccagtgc caatggtggg tttcttcggc gcggtgttat ttggtgcggt
    29161 gatcagtacc ttcaacggct ttctgaatag cgccagtacg ttattcagta tgggtattta
    29221 ccgtcgcatc attaaccaga atgccgagcc gcagcagctg gtcaccgtcg ggcgcaaatt
    29281 tggtttcttt atcgctatcg tttcggtgct ggtcgcgccg tggatcgcca acgcgccgca
    29341 ggggctgtat agctggatga aacagctcaa cggcatttac aacgtgccgc tggttaccat
    29401 catcattatg ggctttttct tcccgcgcat cccggcgctg gcggcaaaag tggcgatggg
    29461 gattggcata atcagctaca tcaccatcaa ctatctggtg aagttcgact tccatttcct
    29521 ctatgtgctg gcctgtacgt tctgcatcaa cgtggtcgtg atgctggtga tcggttttat
    29581 caaaccgcgc gccacgccgt tcaccttcaa agatgcgttt gcggtggaca tgaaaccgtg
    29641 gagaaacgtc aagatcgcgt caattggcat cctgttcgcg atgattggcg tctatgccgg
    29701 gctggctgaa ttcggcggct acggtacgcg ctggttagcg atgatcagtt atttcattgc
    29761 cgccgtagtg attgtctacc tgatttttga cagctggcgg catcgtcacg acccagccgt
    29821 aacctttact cccgacgcga aggatagcct atgaaacgcc ccaattttct gttcatcatg
    29881 accgataccc aggccaccaa tatggtcggt tgctatagcg gtaaaccgct gaatacgcaa
    29941 aatattgata gtctggcggc ggaaggtatt cgctttaatt ccgcctacac ctgttcaccg
    30001 gtttgtacgc ctgcacgcgc cggactattt accggtatct acgctaacca gtccggcccg
    30061 tggaccaaca acgtcgcgct aggcaaaaac atctccacta tggggcgcta ctttaaggat
    30121 gccggctatc acacctgtta catcggcaaa tggcatctcg acggtcatga ctatttcggc
    30181 actggcgagt gtccgccgga gtgggacgct gattactggt tcgatggggc gaactacctt
    30241 agcgaactga cggaaaaaga gatcagcctg tggcgcaatg gcctaaacag cgttgaggat
    30301 ttacaggcga accatatcga cgaaaccttc acctgggcgc accgcatcag caatcgggcg
    30361 gtagattttc tgcaacagcc cgcgcgcgcc gaggaaccct tcctgatggt ggtttcgtat
    30421 gatgagccgc atcacccgtt cacctgtccg gtggagtatt tagagaaata cgctgatttt
    30481 tactacgatc tgggcgagaa agctcaggat gacctggcga acaaaccgga acatcaccgc
    30541 ttatgggcgc aggcgatgcc atcgccagtc ggtgatgacg ggctttatca ccatccgctc
    30601 tattttgcct gtaatgactt tgttgatgac caaatcggac gggtcatcaa tgccttaacg
    30661 ccagagcaac gtgaaaatac gtgggtcatt tatacttccg atcacggcga aatgatgggc
    30721 gcacataagc tgatcagtaa aggagcggcg atgtatgacg acatcacccg tattccgctg
    30781 atcatccgtt cgccgcaagg ggagcggcga caggtcgata cgccagtcag tcatatcgat
    30841 ttactgccga caatgatggc gctggcagat attgaaaaac cagagattct gccgggggaa
    30901 aatatccttg ccgtgaaaga gccacgcggt gtaatggtgg aatttaaccg ctacgagatt
    30961 gagcatgaca gctttggcgg ttttattccg gtgcgttgct gggtgacgga tgactttaaa
    31021 ctggtactca acctcttcac cagtgatgaa ctttacgatc gccgtaatga cccaaatgaa
    31081 atgcataacc tgatcgatga tatccgtttt gcagacgttc gcagcaaaat gcatgacgcc
    31141 ttattggatt acatggacaa aattcgcgat ccgttccgca gttaccaatg gagcctgcgt
    31201 ccgtggcgta aagatgcact gccgcgctgg atgggggcat ttcgtccacg cccacaagat
    31261 ggctattcgc cggtggtacg tgactatgac accggcctac cgacgcaagg agtgaaagtg
    31321 gaagagaaaa aacagaagtt ctgacgataa tgcccgctga tgaacacccg gcgggcatta
    31381 ttcaggctgc ttaaaccagt gaaccgccgg tcaggaagga aaaaatcagg aatatcagca
    31441 aaatgctgca cacgatgccg ataccaaaaa gcaaggtatg gaaagccagc gtcccgccgc
    31501 caaaaaccag cgcgccgcga atgccccagc gattttcctt acgcataccg ccaataaatg
    31561 cgcaaagtag cgcagcaatg gccagtgcga taccgctttg atcgaggatt ttatcgacat
    31621 ctacgtcctc ttttttctcc acggtggtaa ttttttcgcc ttttattccg gcgagaagac
    31681 ctcttttaat ttcagccgtt ttgtccgcca cgatgctttc aaacgttggc ggtggtgcgg
    31741 aaaaagggcc gaatgaaaag tggataatcc ccatcattaa agcgatggca ccgaacagca
    31801 tgcccaatga actgatttta ttttgagcta tgatacccat ctgacctcct tgtagcggat
    31861 ggcgaaagca tatcaggaaa cgggcggacg aaattcttaa aaaaaaaccg ctaaaggtag
    31921 agcgttagtg aaattgcaaa ggagcaacaa tgaaaatttc ccgccttgga gaagcgcccg
    31981 attaccgctt ctcgctggct aatgagcgta cttttctggc gtggatccgt accgcactag
    32041 gatttctggc ggcgggggtt gggcttgatc aacttgcgcc agatttcgcc acgcccgtca
    32101 ttcgtgaact gctggcgttg ttgttgtgtc tgttttccgg cgggttagcg atgtatggct
    32161 acctgcgctg gttacgcaat gaaaaagcga tgcgtctgaa agaggacctg ccctacacca
    32221 acagcttatt aatcattagc ttaatattga tggtcgtggc ggtgatcgtt atgggactgg
    32281 tgttgtatgc cggatagccg caaagccaga cgcattgccg acccaggact gcagccggag
    32341 cgcacatcac tggcgtggtt tcgtaccatg ctgggctacg gcgcgttgat ggcgttggct
    32401 atcaaacaca actggcacca ggcgggcatg ttattctgga tttcgattgg cattctcgcc
    32461 atcgtggcgc tgatcctctg gcactacacc cgtaatcgca atttaatgga tgtcacgaat
    32521 agcgattttt cccaatttca cgtagtccgt gacaaatttt tgatctccct cgcggtgtta
    32581 tctctcgcaa tactgtttgc tgtaacgcat atacatcaac ttatcgtatt tattgagaga
    32641 gtcgcatgac aggaagtcag gtcattgatg ctgaagagga caggcataaa ctggttgtag
    32701 agtacaagga tgctctgcaa cctgccgatt tttatcacaa tttcaaacag cggggcatcc
    32761 gcgccgtaca gctcattccc tatcttgaat ttgatgaccg gggcgatctg acggccgctt
    32821 cggtgacagc agagctgtgg gggaaatttt taattgccct gttcgagtgt tgggtgcggg
    32881 cagatatcag tcgtatctcg attgagcttt ttgacgccac cttacaaaaa tggtgtggca
    32941 gcgaaaaccc gcaaccgcgc cgtgactgcc aggcgtgcga ctggcatcgc ctgtgcccgc
    33001 acgctcgcca ggagacgcca gacagcgtgc tttgtgcggg ctatcaggcg ttttattctt
    33061 actccgcacc gcatatgcgg gtgatgcgtg atctgataaa gcagcatcgt tctcccatgg
    33121 agctgatgac gatgctgcgg tgacgtgcgc ttaaacgggc tgcccctgat ccgacatccg
    33181 cgtcgccagc ggcagccagc acagcacgat caacaatccc attaaggtca tcaacaaccc
    33241 caggctgccc tgaccggttt gcggcaacat cgcagagagc gacgccagca cgccggaacc
    33301 aatgttttgc agaccgccga ccagcgcgcc agccgtgccc gccaggaagg ggaacggctc
    33361 catcgcgccg ctggtcgcca gcggaaacag catcccggca ccgaaaaaga acagcgcggc
    33421 gggaacgagc agcgtccaga cattcatcac gccaaaccag tcggggatcc acatcagcaa
    33481 gccagccagc aggcagcaga taaccgactg ccacattaac gtggagaagc gtttattggg
    33541 acgtccggca aaccatgcgc caaaaaacgc tgccggaatc ggcagaataa acaaaatact
    33601 gaccgtcata ctgctcagcc ctaacaccgc gcccatcagc acgccggagc aggcttcaaa
    33661 ggcggcaatc ccggccagac cgccaatcag catcagcaaa taacagttaa aaccgctgtt
    33721 accgaaaagc gttttataac tggtaagcag gcgcgtgcgc ggtgcatcga ccggacgcgt
    33781 ttccggcatc cagcgggcca tactgaaggt cacaccagca caaagaacca acaaaaagag
    33841 ataacaggcg cgccagttcc acatcgtatc cagcagaccg ccgattagcg gtgcgagcaa
    33901 cggactgacg agaatcccca tgtttaacag gctgttagca tggcgcaact gtgtccgttc
    33961 atataaatct cgcggcaaag tacgcgccat tacgccgcca acgccggtgc ccatcccctg
    34021 catcgcgctg gcggcaatca acaccgtcaa actggaggtc gtgaccgcga ccagcgttgc
    34081 cagcataaaa atggacattc cgacgaggat caccggtcgg cggcccacgc ggtcggaaat
    34141 cgggccataa aacagctgtg agacaccgta agtcagcaga taagcgccca ttacgctctg
    34201 caccgcccct tcacggacgt tgagatcgcg cgccatatcg gcaatagctg gaatataaat
    34261 ggtttgcgcc atctgaccga cggccacgag taataccaac atcaataaca aattgacgtt
    34321 tctttgcctt ttcattatca cggatgcttt tataaaaata attagaataa gtcactacgc
    34381 attcccataa atgcgggaga ggaatctatc acattgatga atgttagcca gatatacgcc
    34441 aggaatgggg aattgtttag cggcagaata tgtaaacaaa agcggcaata aatgttgccg
    34501 ggagggatta cgtgcgaact gtaaccctct ccccaaaggg gcgaggggga aaccaccgtt
    34561 ccctttcaga aaatgagcta aacattgcac gggacggtcc cctcgcccct ttggggagag
    34621 ggctagggtg aggggcacag gcagcgcagg tgcaaacatt ctgctggtag aagaagcccc
    34681 caactccaac ccgcacgcta aatataaaag gggagcggtt acccgctccc ctttggtgcg
    34741 acttgaatct gaattacttc aggtatttca gaacagcatc aagcagttgc agtgctgcaa
    34801 caatgagttt gaggataaga atggcgatat ccaccaggct catacgcgtc tcctgtggtt
    34861 caggagcact gccagctgac gtacctttcc gctgcctgtt gccagctgtt gtgcacgcgg
    34921 tatcttaagg agaaacgttg tgcttgttga cataacacag tgtgctcacg cggggttaag
    34981 gcgctgacgg caccacccgt ttcagccagg acttagtgcg ccgggtaacg atgcggcccc
    35041 cgcataacac attgcgtaca gtgatattat agaagtttac tgtataaata aacagtaata
    35101 tttggacaaa acgcaaactg tgatcgatac tcaactatgc cctaaatacg tcaaaattcg
    35161 tgccgaaatt gcgcgttctg cgcggaacac gtatactttc agtgttgaca taatacagtg
    35221 tgctttgcgg ttaccagccg caggcggctg atgaaacctc gctccggcgg ggttttttgt
    35281 tacctgcaat tcagtacaaa acgtgatcag cccctcaatt ttcccttgct gaaaaatttt
    35341 ccattgtctc ccctgtaaag ctgtgcttgt ataaatattg ttaaacacaa aaccaacaag
    35401 gtccccaatg actacttcca tgttcaacgc aaaactacta ccaactgcgc tatccgccgc
    35461 agtggtcgtc gtgcgtgtgg tggtggtcgt cggcaatgcg ccgtagggac tggaacaaca
    35521 cacgattcca aaaccccgcc ggcgcaaacc gggcggggtt tttcgtttaa gcacctcccg
    35581 gaaagtcggc ccagaagaaa aggactggag catggcaagt tcgggcacaa catcgacgcg
    35641 taagcgcttt accggcgcag aatttatcgt tcatttcctg gaacagcagg gcattaagat
    35701 tgtgacgggc attccgggcg gttctatcct gcctgtttac gatgccttaa gccaaagcac
    35761 gcaaatccgc catattctgg cccgtcatga acagggcgcg ggatttatcg ctcaggggat
    35821 ggcgcgcacc gacggtaaac cggcggtctg tatggcctgt agcggaccgg gtgcgactaa
    35881 cctggtgacc gccattgccg atgcgcggct ggactccatc ccgctgattt gcatcaccgg
    35941 tcaggttccc gcctcgatga tcggcaccga cgccttccag gaagtggaca cctacggcat
    36001 ctctatcccc atcaccaaac acaactatct ggtcagacat atcgaagaac tcccgcaggt
    36061 catgagcgat gccttccgca ttgcgcaatc aggccgccca ggcccggtgt ggatagacat
    36121 tcctaaggat gtgcaaacgg cagtttttga gattgaaaca cagcccgcta tggcagaaaa
    36181 agccgccgcc cccgccttta gcgaagaaag cattcgtgac gcagcggcga tgattaacgc
    36241 tgccaaacgc ccggtgcttt atctgggcgg cggtgtgatc aatgcgcccg cacgggtgcg
    36301 tgaactggcg gagaaagcgc aactgcctac caccatgact ttaatggcgc tgggcatgtt
    36361 gccaaaagcg catccgttgt cgctgggtat gctggggatg cacggcgtgc gcagcaccaa
    36421 ctatattttg caggaggcgg atttgttgat agtgctcggt gcgcgttttg atgaccgggc
    36481 gattggcaaa accgagcagt tctgtccgaa tgccaaaatt attcatgtcg atatcgatcg
    36541 cgcagagctg ggtaaaatca agcagccgca tgtggcgatt caggcggatg ttgatgacgt
    36601 gctggcgcaa ttgatcccgc aggtggaagc gcaaccgcgt gcagagtggc accagttggt
    36661 agcggatttg cagcgcgaat tcccgtgtcc aatcccgaaa gcgtgcgatc cattaagcca
    36721 ttacggcctg atcaacgccg ttgccgcctg tgtcgatgac aatgcgatta tcaccaccga
    36781 tgtgggtcag catcagatgt ggaccgcgca agcttatccg ctcaatcgcc cacgccagtg
    36841 gctgacctcc ggtgggctgg gcacgatggg ttttggcctg cctgcggcga ttggcgcggc
    36901 gctggcgaac ccggatcgca aagtgttgtg tttctccggc gacggcagcc tgatgatgaa
    36961 tattcaggag atggcgaccg ccagtgaaaa tcagctggat gtcaaaatca ttctgatgaa
    37021 caacgaagcg ctggggctgg tgcatcagca acagagtctg ttctacgagc aaggcgtttt
    37081 tgccgccacc tatccgggaa aaatcaactt tatgcagatt gccgccggat tcggcctcga
    37141 aacctgtgat ttgaataacg aagccgatcc gcaggctgca ttgcaggaaa tcatcaatcg
    37201 ccctggcccg gcgctgatcc atgtgcgcat tgatgccgaa gaaaaagttt acccgatggt
    37261 gccgccaggt gcggcgaata ctgaaatggt gggggaataa gccatgcaaa acacaactca
    37321 tgacaacgtg attctggagc tcaccgttcg caaccatccg ggcgtaatga cccacgtttg
    37381 tggccttttt gcccgccgcg cttttaacgt tgaaggcatt ctttgtctgc cgattcagga
    37441 cagcgacaaa agccatatct ggctactggt caatgacgac cagcgtctgg agcagatgat
    37501 aagccaaatc gataagctgg aagatgtggt gaaagtgcag cgtaatcagt ccgatccgac
    37561 tatgtttaac aagattgcgg tgttttttca gtaaccgctc aaggcttgaa caacatcgcg
    37621 cttatcgtta aggtaagcgc gtttttttta cccgccagga caagaccatg atcaccgttg
    37681 cccttataga cgatcacctc atcgtccgct ccggctttgc gcagctgctg gggctggaac
    37741 ctgatttgca ggtagttgcc gagtttggtt cggggcgcga ggcgctggcg gggctgccgg
    37801 ggcgcggtgt gcaggtgtgt atttgcgata tctccatgcc cgatatctcc ggtctggaac
    37861 tgctaagcca gctgccgaaa ggtatggcga caataatgct ctctgttcat gacagtccgg
    37921 cgctggttga gcaggcgctt aacgcggggg cgcgcggctt tctctccaaa cgctgtagcc
    37981 ctgacgaact gattgcggcg gtgcatacgg ttgccacggg cggctgttat ctgacgccgg
    38041 atattgccat taaactggca tccggtcgtc aggacccgct gaccaaacgt gaacgccagg
    38101 tggcggaaaa actggcgcaa ggaatggcgg tgaaagagat tgccgccgaa ctgggcttgt
    38161 caccgaaaac ggtacacgtc catcgcgcca atctgatgga aaaactgggc gtcagtaacg
    38221 acgtagaact ggcgcgccgc atgtttgatg gctggtgatg aagacgttgt tctcccgctt
    38281 aattaccgtt attgcctgct tttttatctt ctctgccgca tggttttgcc tgtggagtat
    38341 cagcctgcac ctggttgagc gccctgatat ggcggtgctg ttatttccgt ttggtctgcg
    38401 gctagggcta atgctgcaat gcccgcgcgg atactggccc gtattgctgg gtgcggaatg
    38461 gctgctgatt tactggctaa cgcaggcggt cggtttaacc cattttccgt tattgatgat
    38521 cggtagttta ctgacgttac tgcccgtagc gctgatctcg cgctatcgcc atcagcgtga
    38581 ctggcgcacc ttgctgttac agggggcggc gttaacggcg gcggcgttgt tgcagtcgct
    38641 gccctggctt tggcacggca aagagtcgtg gaatgcgctg ttgctgactt taactggcgg
    38701 cctgacgctg gccccgatat gtctggtgtt ctggcactat ctcgccaata acacctggct
    38761 gccgctcggc ccgtcactgg tttctcaacc gatcaactgg cgcgggcggc atctggtctg
    38821 gtacttgctg ttgtttgtta ttagtctctg gctccagttg ggattgccgg acgaactgtc
    38881 gcgctttacg ccattctgcc tggcgctgcc gattatcgcg ctggcctggc actacggttg
    38941 gcaaggggcg ctgatcgcga cgttgatgaa cgccatcgcg ctgatcgcca gtcaaacctg
    39001 gcgcgatcat ccggtggatt tattgctctc gttgctggtg caaagtctga cagggttgtt
    39061 actgggcgct ggcatccagc ggttgcgtga acttaaccag tcgctgcaaa aggaactggc
    39121 gcgcaatcag catctggctg aacggttgct ggaaaccgaa gagagcgtgc gtcgtgatgt
    39181 ggcgcgtgag ctgcatgatg atatcggtca gaccatcact gctattcgta ctcaggcggg
    39241 cattgttcag cggctggcgg cagataacgc cagcgtgaag cagagcgggc agctcatcga
    39301 acaactatcg ctgggcgttt acgacgcggt gcgccgtttg ttgggtcggt tacgtccgcg
    39361 ccagttggat gatctcaccc tggagcaggc catccgctca ctgatgcggg aaatggagct
    39421 ggaagggcgc ggtattgtca gccatctcga atggcgaatc gatgaatcag cattaagtga
    39481 aaaccagcgc gtgacgctgt ttcgtgtctg ccaggaaggg ctgaacaaca ttgtgaaaca
    39541 tgctgatgcc agcgcggtca cgctgcaagg ctggcagcag gatgaacggt tgatgctggt
    39601 tattgaagac gatggcagcg gtttgccgcc gggttccggg caacaaggtt ttggcctcac
    39661 cggaatgcgc gagcgcgtaa cggcgctggg tggcacatta cacatttcct gtctgcacgg
    39721 cacgcgtgtc agcgtttctc tacctcaacg ctatgtctaa ggtttgatga tgttgccgtt
    39781 tctgaaagcg cctgccgatg cgccattaat gactgataaa tatgaaattg atgcccgcta
    39841 tcgctactgg cgtcggcata ttctgctgac catctggctg ggttacgcgc tgttttactt
    39901 cacgcgtaaa agttttaacg ccgccgtacc agaaatcctt gctaacggcg tgctcagccg
    39961 tagcgatatc ggcctgttag cgaccctgtt ttacattacc tacggcgtgt cgaagtttgt
    40021 ctccggcatt gtcagcgatc gctcaaatgc ccgttatttt atggggatag ggcttatcgc
    40081 cacgggcatc atcaacattc tgtttggctt ctcgacgtcg ctatgggcgt ttgccgtgct
    40141 ctgggtgctg aacgcctttt tccagggctg gggttcaccg gtgtgtgcgc gtctgttaac
    40201 ggcctggtat tcacgtaccg agcgcggcgg ttggtgggca ttatggaaca cggcgcataa
    40261 cgtcggcggc gcactcattc ccattgtgat ggcagcgagc gcgctgcatt acggctggcg
    40321 tgccgggatg atgattgctg gttgtatggc gatagtcgtg gggatttttc tctgctggcg
    40381 gctacgcgat cgcccgcagg cgttaggttt accggcggtc ggtgaatggc ggcacgacgc
    40441 gctggaaatt gctcaacaac aagaaggggc agggttgacg cgtaaagaga tcctgaccaa
    40501 atatgtgttg ctgaatccgt atatctggct gctttcgttt tgctatgtgc tggtctatgt
    40561 ggtccgggcg gcgatcaacg actggggcaa tttgtatatg tccgagacac tgggcgtcga
    40621 tctggtcacg gcgaatacgg cagtgacgat gtttgaactg ggcggattta tcggtgcgct
    40681 ggtagccggt tggggctcgg acaaattgtt taacggcaac cgtgggccga tgaatttgat
    40741 tttcgccgcc ggaattttgc tttcagtcgg ctcgctgtgg ctgatgccat ttgccagcta
    40801 cgtgatgcag gcaacctgct tcttcaccat tggttttttt gtctttggcc cgcagatgtt
    40861 aatcggcatg gcggcggcag agtgttccca caaagaggcg gcaggggcgg cgacggggtt
    40921 tgtcggcttg tttgcttatc tgggggcgtc gcttgctggt tggccgctgg cgaaagtact
    40981 cgatacctgg cactggagcg gattttttgt ggttatctct atcgccgccg ggatttccgc
    41041 actgctgtta ctgccctttt tgaacgccca ggcaccgcgc gaagcgtgat gcatctcacc
    41101 ttttcacttc atatccggca aaactaagaa attttccagg ttttgcctgg acgctatctc
    41161 aggcctgatt tgctgctgat ttttacaatg catgcctcac gcaggtattc atttcaggag
    41221 taacccatgc tggctttctt aaaccaggtt cgcaagccga ccctggacct tccgctcgaa
    41281 gtgcggcgca aaatgtggtt caaaccgttc atgcaatcct acctggtggt ctttatcggc
    41341 tacctgacga tgtacctgat tcgcaagaac tttaacatcg cgcagaacga catgatctcg
    41401 acctacgggt tgagcatgac gcagctgggg atgatcggcc tgggtttctc catcacttat
    41461 ggcgtgggta aaacgctggt ttcctactac gccgacggca aaaacaccaa acaattcctg
    41521 ccgttcatgc tgatcctgtc tgccatttgt atgctgggct tcagtgccag tatgggcagc
    41581 ggctcggtta gcctgttcct gatgattgcc ttctacgcct taagcggctt tttccagagt
    41641 accggcggtt cgtgcagtta ctccaccatc accaaatgga caccgcgtcg taagcgcggg
    41701 accttcctcg gtttctggaa catttcccat aaccttggcg gcgcgggtgc ggctggtgtg
    41761 gcgctgttcg gcgcaaatta cctgttcgac ggccacgtga tcggcatgtt tatcttcccg
    41821 tcgattatcg cgttgattgt cggttttatc ggcctgcgtt acggcagcga ctccccggaa
    41881 tcttatggcc tcggcaaagc ggaagagctg ttcggcgagg agatcagcga agaggacaaa
    41941 gagacagaat ctaccgatat gaccaagtgg cagatctttg ttgagtatgt gctgaaaaac
    42001 aaagtgatct ggctgctgtg cttcgccaac attttcctct atgtggtgcg tattggtatc
    42061 gaccagtggt caaccgtgta cgcgttccag gaactgaaac tctctaaagc ggtggcgatt
    42121 cagggcttta cgctgtttga agctggtgcg ctggtcggta cgctgctgtg gggctggctc
    42181 tctgacctgg cgaacggtcg ccgtggcctg gtggcctgca tcgcgctggc gctgattatc
    42241 gccacgctcg gtgtgtatca acatgccagc aacgaatata tctatctggc ttctctcttt
    42301 gcgttgggtt tcctggtctt tggcccgcaa ttgttgattg gtgtggctgc tgttggcttt
    42361 gtacctaaaa aagcgattgg cgctgccgat ggtattaaag gcacctttgc ttacctgatt
    42421 ggtgacagct ttgccaagtt aggtctggga atgattgccg atgggacgcc ggtattcggc
    42481 cttaccggtt gggcaggcac cttcgccgca cttgatatcg ccgcgattgg ttgtatctgc
    42541 ctgatggcga tagtggcggt gatggaagaa cgcaaaatcc gccgcgagaa aaaaattcag
    42601 cagttgacag tggcataaac gtaactggtg acttttgccc ggcatgacgc cgggcttttt
    42661 tattattccg tgacttccag cgtagtgaag gcaaacttct cgccatcaaa tagcccctga
    42721 ctggttagtt ttagcgcggg gatcactggc agagaaagaa acgccatctg aataaacggc
    42781 tcatcgggta acggaccgca ttcacgggcg gcggctttca aggcgtcaat ttgttccgcc
    42841 agcaactgcg ccgtgtcggt gctcatcagg ccggcaatgg gcaacggcag atgactttgg
    42901 acctggccgt tacgtaccac gcacagcccg ccgccatcct gaatcacctg attgaccgcc
    42961 agcgccatct cttcggcact gcgaccgatc accacaatat tatggctgtc atggctgacc
    43021 gtcgcagcca gcgcaccttc attcagtcca aagccgccaa gcaaaccaca agccggagcc
    43081 agccgttgcc cgtaacgctc aagtacggca ataaagctca catcatcgcg atcaaaacca
    43141 ttttcgctgt agacgctgga gtgggagtgc gtaatcaatt cgttatggat gacgtcaatg
    43201 acccgatagc gttttccggg cgtaaattgc agggcaaagt cgctggcgga aactggctgg
    43261 cgggcaatgg tgttgccata tggcggagcg gattgtgcca gtctcgccga ctcttccgcc
    43321 tgtaaggttt gcgcatcaat cggctcgcct ttcaccagta cctgctgcac cgtgacctta
    43381 cgcgcatcgc tcaacaggac gatatcggcc tgcttgccgg gtgccagtaa gccgaggtga
    43441 ttcagaccaa agtggcgcgc cgtcgaccag ctggcgacgc gatatgccac atgcagcggc
    43501 acattgtgtt gttcgatcag gcggcgaatt aaggcatcga tgtgtccttc atgggcgatc
    43561 tcccacgggt tacggtcatc ggtacagagc atgcattgcg ggctgttaaa ttcgttgatc
    43621 aacggtgcca gcgcgttgag attgcgggca gcggaccctt cgcggatcat caacgacatg
    43681 ccgagttgta atttccggcg tccttcttcc agctgataac tttcgtggca gttttcaata
    43741 cccgcagtaa tataggcgtt aagttcttta ccacccaaac ccgggcagtg accgtccagc
    43801 gtcaggtggc gaaatgcatc cagtttatcg agcagcgcat tctgcccgct aattacgcca
    43861 gggtagtcca tcatttctgc aaggccggta acctgcggat ggtcccgcca ggcgagcatc
    43921 tgttcaaggg taaaactggc accgttaaca tcgcagcctt ccagggcggg tacgcaagag
    43981 ctgacctgta agtactggtt ttgccttgcc tgttcggcac agcgggcaaa ccaggcgaat
    44041 ccggcttctc ccatcacgtt gacgatttca tgagggtcgc aaataacggt cgtcaggccg
    44101 cgcggcaggg tagcggtttc aaaagtgacc ggcgtcatca tgctggattc aatatgcagg
    44161 tgagcatcaa taaaccctgg caccgccgtt gcgccgcgag catcaatccg ctgcaaagcc
    44221 ggagcatcag cgtattctgc gccaacaccg gcaatgtaac gtcctttaat cacaattggg
    44281 ccggaaattt ctccgccatt gatcaggtcg agaatagaga cattatcaat aatataatcg
    44341 gcaacagcgt cgccacggga aacggctaac aattcctggt attcagcccg gctaatgtga
    44401 tgaaatttat ggttaataga attattcata tttttctcat tttgatttta tcaatttgat
    44461 tattatttat cataaagaaa cgtcgcgaga ttattaaaga atatccatta atgtgcaatt
    44521 gaaatgtgat aattatcact gaaccgttgt tgtcatttgt tttacaaaag caagggatta
    44581 aatctcctcc aatggacaaa aaaatgaata atgacaatac cgattacgtg agtaatgaat
    44641 cagggacgct ttcgcgatta tttaaactac ctcagcatgg gaccaccgtc cgcacagaat
    44701 tgattgcggg gatgaccact tttttaacca tggtgtacat cgtttttgtg aacccgcaaa
    44761 tcctcggcgc ggcacaaatg gacccgaaag tggtgtttgt taccacctgt ttgattgccg
    44821 gtatcggcag tattgcgatg gggatatttg ctaacttacc cgtggcgctg gctccggcaa
    44881 tggggctgaa cgccttcttt gccttcgtgg tcgtgggggc gatgggcatc tcctggcaga
    44941 ccgggatggg cgcaatattc tggggcgcag ttggactatt tttgctcacg ctgtttcgta
    45001 tccggtactg gatgatctcc aacattccct taagtttacg tattggtatc accagcggaa
    45061 ttggattatt tatcgcctta atgggattaa aaaatactgg cgttattgtc gccaataaag
    45121 acacgctggt gatgattggc gatttaagtt ctcacggcgt gttgttaggt attttagggt
    45181 tttttattat aaccgtgttg tcatcacgtc attttcatgc cgcggtgctg gtttctattg
    45241 tggtgacgtc ttgctgtgga ttatttttcg gtgatgttca ttttagcggc gtctattcca
    45301 ttccgcctga tattagcggc gtcattggtg aagtagattt gagcggcgcg ttaacacttg
    45361 aactcgccgg tatcattttc tcctttatgc tgatcaacct atttgattca tcaggaacat
    45421 taattggtgt aactgataaa gcgggcttaa tagatagtaa cggtaaattc cccaatatga
    45481 ataaggcgct gtatgttgat agcgtcagtt cggtggcggg ggcgtttatc ggcacctcgt
    45541 ctgttactgc ctatattgaa agtacttctg gtgtggcagt cggtggtcgc acggggctga
    45601 ctgcggttgt ggttggcgtt atgttcctgt tagttatgtt cttctcaccg ctggtggcga
    45661 tggttcctcc ttacgcaacc gccggagcgt taatctttgt tggcgtgctg atgacttcga
    45721 gcctggcgcg cgttaactgg gatgatttta ccgaatcggt gcctgcgttt attaccacgg
    45781 tgatgatgcc ctttactttc tcgatcaccg aagggattgc actcggcttt atgtcgtact
    45841 gcatcatgaa agtatgcacc gggcgctggc gcgatctgaa cctgtgtgtg gtggtggtcg
    45901 cagctctgtt tgcactgaag attattctgg tggattagta actggcgagg gtttccagat
    45961 atcatgagtt ctgattacgc aggagaactc atgatctgga taatgctcgc cacgctggcg
    46021 gtagtgtttg tggttggttt tcgggtgctg acatccgggg ccagaaaagc gattcgccgt
    46081 ctcagcgatc ggctgaacat cgatgtcgta cccgtggagt cgatggtcga tcaaatggga
    46141 aagtcagctg gtgacgaatt tttacgttat ttgcatcgtc cggatgagtc gcacctgcaa
    46201 aacgccgcgc aggtgttgct catctggcaa attgtcattg tcgatggtag cgaacagaac
    46261 ctgctgcaat ggcatcggat tttacaaaaa gctcgcctcg ccgcgccgat taccgacgct
    46321 caggtcaggc tggcgctagg ttttctgcgc gaaaccgaac ctgaaatgca ggatattaat
    46381 gcttttcaga tgcgctataa cgcgttcttt cagcctgccg agggcgttca ctggttgcat
    46441 tgaaaccgtt tcgtcttatc agcctacaga ccgcgcctga cctgtcgggt ggataagacg
    46501 ttcacgccgc atccggtaat ctttgccctg ttccgtttcg atcttaactc cagctcaagc
    46561 aacaccgtaa aagttgcaaa aacgttaaat gcgtcacaca tttcaatgtt aaagtggtcg
    46621 gcttttcccc tgaaacatgc cacgggtaac accatgagtg aatttattgc cgaaaaccgc
    46681 ggcgcggatg ccatcacccg accgaactgg tcagccgttt tctcggtggc gttttgtgtc
    46741 gcctgtctga ttatcgttga gtttttgccc gtcagtttgt tgacgccaat ggcccaggat
    46801 ttaggcattt cggaaggggt tgccgggcaa tcggtgaccg tgaccgcctt tgtggcaatg
    46861 tttgccagtt tgtttattac ccagacaatt caggctactg accgccgcta cgttgttatt
    46921 ttgtttgccg ttttgctgac gctctcctgc ttgctggttt cctttgctaa ctcattcagt
    46981 ttgcttttaa tcggtcgtgc ctgtctgggg ctggcgctgg gcgggttctg ggcgatgtcg
    47041 gcgtcgctga ccatgcgtct ggtgccgccg cgtacggtgc cgaaggcgct gtcggtgatc
    47101 ttcggcgcgg tttctattgc gctggtgatt gccgcgccgt tgggcagttt tttaggcgag
    47161 cttatcggtt ggcgcaatgt ctttaatgcg gcggcggtga tgggcgtgct gtgtattttc
    47221 tggattatca aatcattgcc ttcactgcca ggcgaaccct cgcatcagaa acaaaatact
    47281 ttccgcttat tacaacgtcc gggtgtgatg gcagggatga tcgccatctt catgtctttc
    47341 gccgggcagt ttgctttctt cacgtatatt cgcccggtgt atatgaacct ggcgggattc
    47401 ggcgtggatg gcttaacgct ggtgctgttg agttttggta tcgccagctt tattggtacg
    47461 tcgctttcgt cgttcattct taaacgttcg gtaaaactgg ccttagcagg cgcgccgtta
    47521 atactggctg tgagtgcgtt ggtactgacg ttgtggggaa gcgataaaat cgttgctacc
    47581 ggcgtggcga ttatctgggg gctaactttt gcattggttc ccgtcggctg gtcaacgtgg
    47641 atcacccgct cgctggccga tcaggcagaa aaagccgggt ctattcaggt ggcggttatt
    47701 cagcttgcta atacctgtgg cgcggcaatc ggcggttatg cgctggataa tattggtctg
    47761 acttcgccgt tgatgttgtc cggcacattg atgttgctga ctgcattgtt ggttactgca
    47821 aaggtgaaaa tgaagaaatc ctgatatcag gtaaagaagc gttggtcagt agttgttgct
    47881 gttcttacat atccgggcat tctaccttac ccaacgcttc ccaataattg ctctgattat
    47941 tacgttccat cgccacgatg gcctcgcgta tatgttgttt attgttttca tctgacaaca
    48001 ttaacttttc ccacgcttca tctgataatg acgcctgtgg cgtgcaaatt ttacgtaaat
    48061 cttgtaatac cgtggttttc tcttttgcgg actgcagtga gctaaaaggc gactcggcgt
    48121 atacgatgcc tagcatagcg aaaaaagtga aaataagcag tagtgtcgtt ggtttcatgg
    48181 attcaacctt ttacaaatgt gtatcgaatg aagcataagt gtagtttgct ttcatcgctc
    48241 tcatcatgct gtacgaaacg cgcttgcgtg gtcagtaaga agtgccagac tttatattcc
    48301 acttttattc ctttttattc ttatcgatag cgtttcgttt tttaaaccgc agcgacctta
    48361 ccgctatagt caggtaatca ttaataaaag gataaaaaaa tgaaactgac aacacatcat
    48421 ctacggacag gggccgcatt attgctggcc ggaattctgc tggcaggttg cgaccagagt
    48481 agcagcgatg caaaacacat taaagttggc gttataaatg gcgcagaaca agatgtcgcg
    48541 gaagtcgcta aaaaggtggc aaaagagaaa tatggcctcg atgttgaact ggtaggattt
    48601 agcggttcgt tgctaccgaa cgatgcgaca aatcatggcg aacttgatgc gaatgtcttc
    48661 cagcatcgac cctttcttga acaagataat caggcgcatg gttataaact ggtagcggtg
    48721 ggaaatacgt ttgtcttccc tatggcgggt tattccaaaa aaatcaaaac cgtggcgcaa
    48781 ataaaagaag gcgcgacagt ggcgatcccc aacgacccga ccaaccttgg tcgcgcactt
    48841 ttactgttgc aaaaagagaa gctaattact ctgaaagagg gaaaagggtt attacctacc
    48901 gcgctggata taaccgataa tcctcgtcat ttgcagatta tggaactcga gggggcgcag
    48961 ctcccgcgcg tactggatga tcccaaagtt gatgtagcga ttatcagcac cacttacatt
    49021 cagcagacag ggctttctcc ggtgcacgac agcgtattta ttgaagataa gaattcgccg
    49081 tatgtgaata ttttggtggc acgggaagat aataagaatg cagaaaacgt gaaggaattt
    49141 ctgcaatctt atcaatcacc cgaagtcgct aaagcggcag aaaccatttt taacggtggc
    49201 gcggtgcctg gctggtaatt ctcactcatg ccgcgccgga cgatttattt ttctggcgcg
    49261 gcgacgggaa tccattgaaa tcaaaatcac cgatgacaga atcagcagcg ttcccagcca
    49321 gtccggtaat gtgaacgtga tccccagcaa cagcaaagag agtagcgcgc tacttaatgg
    49381 ttctgcacag ctcaaaatgc tggcttttgg accgccaatt aattgtgctc ctttcaggta
    49441 cagactaaat gtcagggacg taccaatgac caccaaataa aaaaacgcca gaatcaaact
    49501 gccgttaacg acaaagtttg ttccttgtct ggcataaaaa ggcaacagaa tcagaccgcc
    49561 aatcagcata ctccagccga cgactggtaa cgtgccatag cgggcaatta gcgttgaggg
    49621 ataggtggta tagaatgcag cagcaaatgc cgaggcaatg ccccagaaca acgcggcagg
    49681 agagatcgat aatgacgtcg gattaccgtg tgtcaccaat aaaaaagtcc cgaccagcga
    49741 tgtcaaaata gcgcagaaaa ccagaatgcc cgggcgcgat ttacgcacca gtgagaacca
    49801 ggcgacgata atcgtcggtg agaggaattg cagcaccgtt gccgtggctg cgttcgattt
    49861 ttcgatggtt agcaaaaaag tgagctgtac agttagcgcg ccaaccacgg aaaaaatcag
    49921 caggctaatg gcatctttat gattgttaat aatagaaaag attttatcgc catgaacaaa
    49981 tgacagcgtc agtagaatca aaccggcgaa tatcaaacgc gtcatagtca aaaactgcga
    50041 cgacatctgg ctttgctcca tgatgtattg cgcgcagacc cctgaacttc cccacaacac
    50101 ggcggcaatc agaacgttca gcatcccctt tctggtggaa cccatttttc cctctgcata
    50161 ctgttttgtc gtttatgtat gcaggcatga tagcaaaaat ggcgaggatg gctctgtttg
    50221 cgccacgccg atttttctta gcccagactg gtgagctaaa tatctttaat aaacagcagg
    50281 caaatcatcg caatacccaa catccctatc gccagccaga acaacgcatg gtagctccag
    50341 atttcaacca taattccgtc aacagagccg gcgataatcc agccgacgcg tgaagtattt
    50401 gcatataacg ttgtcgcaga gcctattttt tcaggcatca agtcctgaaa ataaagcatg
    50461 ccgataccac agagaatacc aaggaagatg gcattaagaa tttgcaattc cagctcaacc
    50521 gccggagtcg tcgccatgag tacgctggcg taaaaacaca ttccactcac gatagcaatg
    50581 agcattaata gtcgcttgcc aatacgtttc atgtaatagc ctgcgattaa catcatcgga
    50641 atttccagac cggcagcgat accaatcatt tctccagcca gtttatcggt tagatgcagt
    50701 tcatcaataa taaatagcgg catatttatc atgtagagat tattcgccgc ccacatcatt
    50761 gaacagacca caaaaagtag ccgcgtatcc cgcttcctgt gggtggaggg taaaatttct
    50821 acgggttggg taacgacagg aatatttctt tgtattgatg gcaaaaacaa ccagactatc
    50881 agtccgcaaa caacaaatgc gatggctgcg gtgagataca tcactttaaa actaaatccc
    50941 attgccagtt cataagcgag cggtggcccg ataacccagg caagcgagat ctgagcacgt
    51001 aaaaatgtac tgaacatgac cgtctcacgg cctgttctgt cggcgtgttc acgggcgagg
    51061 gcgaacattt gcgggtttgc ggtggaagca aaactactca gaagtacgcc cgttgagagg
    51121 agaatgaagt agttgcgatt ccacgcaaaa agcgtgcagg ccagcactcc aaataagcaa
    51181 catagcagaa tcagtaattt acggtcgcct tgtttatcgg agtgccttgc cagaaattga
    51241 ctgaccagaa ttcccataat agcgctaccg gtgaagaaaa aacctaccat tataggacgg
    51301 gctttcagtt catctgcgag gaatatactt agggtaggag tctgaagagc gcccgcaata
    51361 cccgtcagaa aggcgacaag taaaaatgcc gcggcagtga gatcaagtat ttttgatgga
    51421 gtggtagccg ttttttgcat tcttctgaat caccgggcga aaaaatataa ctgagttaca
    51481 aataagtacc agtcgaatga gtattgtatt aagaattttt gagtatcgct gatacaacca
    51541 gaaagcaatg ctagcgcaat attat
//
LOCUS       DAZGVQ010000012        51012 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000012 DAZGVQ010000000
VERSION     DAZGVQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51012
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.012"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            12..1061
                     /gene="yeiH"
                     /locus_tag="ACHLAG_000681"
     CDS             12..1061
                     /gene="yeiH"
                     /locus_tag="ACHLAG_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFP0653970.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAVGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1135..1992
                     /gene="nfo"
                     /locus_tag="ACHLAG_000682"
     CDS             1135..1992
                     /gene="nfo"
                     /locus_tag="ACHLAG_000682"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFP0653971.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            1996..3084
                     /locus_tag="ACHLAG_000683"
     CDS             1996..3084
                     /locus_tag="ACHLAG_000683"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP0653972.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3140..4390)
                     /locus_tag="ACHLAG_000684"
     CDS             complement(3140..4390)
                     /locus_tag="ACHLAG_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP0653973.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(4490..5431)
                     /gene="rihB"
                     /locus_tag="ACHLAG_000685"
     CDS             complement(4490..5431)
                     /gene="rihB"
                     /locus_tag="ACHLAG_000685"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFP0653974.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            5561..6259
                     /gene="yeiL"
                     /locus_tag="ACHLAG_000686"
     CDS             5561..6259
                     /gene="yeiL"
                     /locus_tag="ACHLAG_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFP0653975.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLTKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(6330..7580)
                     /gene="psuT"
                     /locus_tag="ACHLAG_000687"
     CDS             complement(6330..7580)
                     /gene="psuT"
                     /locus_tag="ACHLAG_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP0653976.1"
                     /translation="MDIMRSVVGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(7674..8612)
                     /gene="psuG"
                     /locus_tag="ACHLAG_000688"
     CDS             complement(7674..8612)
                     /gene="psuG"
                     /locus_tag="ACHLAG_000688"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFP0653977.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(8600..9541)
                     /gene="psuK"
                     /locus_tag="ACHLAG_000689"
     CDS             complement(8600..9541)
                     /gene="psuK"
                     /locus_tag="ACHLAG_000689"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFP0653978.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(9996..11687)
                     /gene="fruA"
                     /locus_tag="ACHLAG_000690"
     CDS             complement(9996..11687)
                     /gene="fruA"
                     /locus_tag="ACHLAG_000690"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFP0653979.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWQGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVDASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISECAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(11704..12642)
                     /gene="fruK"
                     /locus_tag="ACHLAG_000691"
     CDS             complement(11704..12642)
                     /gene="fruK"
                     /locus_tag="ACHLAG_000691"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP0653980.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(12642..13772)
                     /gene="fruB"
                     /locus_tag="ACHLAG_000692"
     CDS             complement(12642..13772)
                     /gene="fruB"
                     /locus_tag="ACHLAG_000692"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFP0653981.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            14140..15321
                     /gene="setB"
                     /locus_tag="ACHLAG_000693"
     CDS             14140..15321
                     /gene="setB"
                     /locus_tag="ACHLAG_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFP0653982.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(15318..15572)
                     /gene="yeiW"
                     /locus_tag="ACHLAG_000694"
     CDS             complement(15318..15572)
                     /gene="yeiW"
                     /locus_tag="ACHLAG_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP0653983.1"
                     /translation="MKCRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            15727..16299
                     /gene="yeiP"
                     /locus_tag="ACHLAG_000695"
     CDS             15727..16299
                     /gene="yeiP"
                     /locus_tag="ACHLAG_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFP0653984.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            16522..17988
                     /gene="yeiQ"
                     /locus_tag="ACHLAG_000696"
     CDS             16522..17988
                     /gene="yeiQ"
                     /locus_tag="ACHLAG_000696"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP0653985.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGCE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            18106..19092
                     /gene="yeiR"
                     /locus_tag="ACHLAG_000697"
     CDS             18106..19092
                     /gene="yeiR"
                     /locus_tag="ACHLAG_000697"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFP0653986.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSTLLKLRLATTA"
     gene            19131..19843
                     /gene="lpxT"
                     /locus_tag="ACHLAG_000698"
                     /pseudo
     CDS             19131..19843
                     /gene="lpxT"
                     /locus_tag="ACHLAG_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
     gene            20255..20821
                     /gene="mepS"
                     /locus_tag="ACHLAG_000699"
     CDS             20255..20821
                     /gene="mepS"
                     /locus_tag="ACHLAG_000699"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFP0653987.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            21002..22558
                     /gene="pdeN"
                     /locus_tag="ACHLAG_000700"
     CDS             21002..22558
                     /gene="pdeN"
                     /locus_tag="ACHLAG_000700"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP0653988.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            22640..24454
                     /gene="yejA"
                     /locus_tag="ACHLAG_000701"
     CDS             22640..24454
                     /gene="yejA"
                     /locus_tag="ACHLAG_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP0653989.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLLLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            24455..25549
                     /gene="yejB"
                     /locus_tag="ACHLAG_000702"
     CDS             24455..25549
                     /gene="yejB"
                     /locus_tag="ACHLAG_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFP0653990.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            25549..26572
                     /gene="yejE"
                     /locus_tag="ACHLAG_000703"
                     /pseudo
     CDS             25549..26572
                     /gene="yejE"
                     /locus_tag="ACHLAG_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            26574..28163
                     /gene="yejF"
                     /locus_tag="ACHLAG_000704"
     CDS             26574..28163
                     /gene="yejF"
                     /locus_tag="ACHLAG_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFP0653991.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(28167..28511)
                     /gene="yejG"
                     /locus_tag="ACHLAG_000705"
     CDS             complement(28167..28511)
                     /gene="yejG"
                     /locus_tag="ACHLAG_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFP0653992.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNRLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(28844..30034)
                     /gene="bcr"
                     /locus_tag="ACHLAG_000706"
     CDS             complement(28844..30034)
                     /gene="bcr"
                     /locus_tag="ACHLAG_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFP0653993.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(30062..30757)
                     /gene="rsuA"
                     /locus_tag="ACHLAG_000707"
     CDS             complement(30062..30757)
                     /gene="rsuA"
                     /locus_tag="ACHLAG_000707"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFP0653994.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRECIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            30906..32666
                     /gene="radD"
                     /locus_tag="ACHLAG_000708"
     CDS             30906..32666
                     /gene="radD"
                     /locus_tag="ACHLAG_000708"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP0653995.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHEHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            32791..33075
                     /gene="rplY"
                     /locus_tag="ACHLAG_000709"
     CDS             32791..33075
                     /gene="rplY"
                     /locus_tag="ACHLAG_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFP0653996.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(33214..34221)
                     /gene="yejK"
                     /locus_tag="ACHLAG_000710"
     CDS             complement(33214..34221)
                     /gene="yejK"
                     /locus_tag="ACHLAG_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFP0653997.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            34403..34630
                     /gene="yejL"
                     /locus_tag="ACHLAG_000711"
     CDS             34403..34630
                     /gene="yejL"
                     /locus_tag="ACHLAG_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFP0653998.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            34650..36410
                     /gene="yejM"
                     /locus_tag="ACHLAG_000712"
     CDS             34650..36410
                     /gene="yejM"
                     /locus_tag="ACHLAG_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFP0653999.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            36485..36561
                     /locus_tag="ACHLAG_000713"
     tRNA            36485..36561
                     /locus_tag="ACHLAG_000713"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36519..36521,aa:Pro,seq:ggg)
     gene            36780..38021
                     /locus_tag="ACHLAG_000714"
     CDS             36780..38021
                     /locus_tag="ACHLAG_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFP0654000.1"
                     /translation="MARKTHPLTTVQIKAARPAQKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLEDVSLADARKRRSEYRTLISAGTDPQDHERQKRETEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            38120..38350
                     /locus_tag="ACHLAG_000715"
     CDS             38120..38350
                     /locus_tag="ACHLAG_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654001.1"
                     /translation="MQKNIRCNCDGLQLALMVQHEFWSTYDPEDRTTAPSKKQVVDFL
                     VSRGASRNLAVSIDKVVRPASMKIGGRPKKWR"
     gene            38518..38724
                     /locus_tag="ACHLAG_000716"
     CDS             38518..38724
                     /locus_tag="ACHLAG_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0654002.1"
                     /translation="MEAIRKIIFRQEVKKIIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEDDVLSWQKEREEEARGTAA"
     gene            38739..39404
                     /locus_tag="ACHLAG_000717"
     CDS             38739..39404
                     /locus_tag="ACHLAG_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654003.1"
                     /translation="MTNTKKAAPIWSRLSEQLTRCALCVCDPKHKHGDDSRYQAGGQC
                     NQSGSVRCHTCNERFSLYSLRNCSRAKAHGANLSDSCSIFLRRLFRAGDVFSVDSSLV
                     IVCACKAMRRSTSHHGADSFYPLGSMPRCCNSLRIIRFIQAARESSPSCCCAFSIFSR
                     KSGSKRNWNGGLPRLSFLCVDTLITPHVMYLCVMTHYTQSTQKAKPRTVGAVTGLLTN
                     NVN"
     gene            39413..39751
                     /locus_tag="ACHLAG_000718"
                     /pseudo
     CDS             39413..39751
                     /locus_tag="ACHLAG_000718"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
     gene            39782..39970
                     /locus_tag="ACHLAG_000719"
     CDS             39782..39970
                     /locus_tag="ACHLAG_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0654004.1"
                     /translation="MATIPTLVHSQTAFIWRFIIFGASESQIIHVTAWTEREARSRCP
                     SGCVAVFAARIRQGSHHA"
     gene            39963..40427
                     /locus_tag="ACHLAG_000720"
     CDS             39963..40427
                     /locus_tag="ACHLAG_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP0654005.1"
                     /translation="MRKNRLQKIITGLYASPVAHMATGFSSLNTTADSRLHSDMRFFC
                     VRKPTHTRIMAGRNGGAFALAGSCSASLLTLLRLATPFSSVVARLLNFTTGAFHMAVF
                     TRPYFVWRFMQCLTDSIAIFTVTAATEREARAQLPHAHLIFVARIRQGVCHA"
     gene            40420..40740
                     /locus_tag="ACHLAG_000721"
     CDS             40420..40740
                     /locus_tag="ACHLAG_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654006.1"
                     /translation="MPDMTNYQYLINPHFNCEHDIAKKVYSAADGATDNISMAVSSIG
                     SLMWYASENKEYDEKAMRIDMGNIGLLLAMLGQFDISLRCTIENAKDALNAIKKANTD
                     SNRG"
     gene            40747..41046
                     /locus_tag="ACHLAG_000722"
     CDS             40747..41046
                     /locus_tag="ACHLAG_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0654007.1"
                     /translation="MRTYLSGLTASGPARPEIRPGDIFRDNYGGVVEIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFSLVQAAPRSKPTSREKARANIQEIRKMLNVFRGKK"
     gene            41043..42791
                     /locus_tag="ACHLAG_000723"
     CDS             41043..42791
                     /locus_tag="ACHLAG_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HFP0654008.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWAHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHPESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENICDNAPNFH
                     KWLEHAAGKDPRKMMRICAALYMIMANRYDWQMFIEATGEGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVSRRRVIFRFDNIVREDEKDKDLPEKVAAEIP
                     VIIRRLLANFADPEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVAEFSKAMKPAAKVYGHEYITRRVKG
                     VTQTNAITTDDCDAFL"
     gene            43078..43345
                     /locus_tag="ACHLAG_000724"
                     /pseudo
     CDS             43078..43345
                     /locus_tag="ACHLAG_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            43353..43625
                     /locus_tag="ACHLAG_000725"
     CDS             43353..43625
                     /locus_tag="ACHLAG_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0654009.1"
                     /translation="MIHDRIAEELEAKGFYRRASARWGEVMLLVETDKERHQVTMRRL
                     ECSRKAQKPPEPPDNFGDLRKAVDRTYAEMGIDGVSDEIWRNYPDS"
     gene            43866..44072
                     /locus_tag="ACHLAG_000726"
     CDS             43866..44072
                     /locus_tag="ACHLAG_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654010.1"
                     /translation="MAEEKKGGVSVYISPDIVKALKERHQQNVKAGIAAGLDPLAMVE
                     PSTGWQVRAYLRAALGMNQVHGGE"
     gene            44072..45127
                     /locus_tag="ACHLAG_000727"
     CDS             44072..45127
                     /locus_tag="ACHLAG_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP0654011.1"
                     /translation="MTVKAIALNTNQLFAYLNREDIAEFKFSPLFTALFFPNVATFNT
                     QDIMLDNLDIEEVTMSAFCSPMVGSQVQRDKGYETSIIRPGYMKPKHEIDPLKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPEKNIIEQADGKKWSEQDKDIHDPIYDIELYADQAGCPANVMIMGVDVWRMLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDLALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMEQEAVRTGATQNMYYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            45139..45474
                     /locus_tag="ACHLAG_000728"
     CDS             45139..45474
                     /locus_tag="ACHLAG_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP0654012.1"
                     /translation="MNTPYVELFAGSQQVSTTLVHFAADAGVIQEFTPLMLADNGEFK
                     PWDGQESGKAVYLTSYPVDTSTQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            45487..45900
                     /locus_tag="ACHLAG_000729"
     CDS             45487..45900
                     /locus_tag="ACHLAG_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFP0654013.1"
                     /translation="MATNESIMTLPLASKFKAEASAMADRGLSTYEAVYQLNKLEEQD
                     KPRADAIMALHEHKDYQPLLRAMANVPCISVDNAREILNMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNQGDDGDIDRILKTI"
     gene            46107..46649
                     /locus_tag="ACHLAG_000730"
     CDS             46107..46649
                     /locus_tag="ACHLAG_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP0654014.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IENRVNNAISQLINDKGEIDDDELLRVRIRLLTAQAEAQELKNDRDRGDVIDTEFCLY
                     ALSKLASQISSIMDSLPLTMQRSFPQITPAMLDRLKREVVKACNASARVADNLPQILA
                     DYLKETTGNVPEKLLQKKGE"
     gene            46905..47186
                     /locus_tag="ACHLAG_000731"
     CDS             46905..47186
                     /locus_tag="ACHLAG_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654015.1"
                     /translation="MTEAEILGLIRRVAGISQQVDEQATQPDSVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRVEMYREKEKITYHWKKPERLRR"
     gene            complement(48333..50924)
                     /gene="yejO"
                     /locus_tag="ACHLAG_000732"
                     /pseudo
     CDS             complement(48333..50924)
                     /gene="yejO"
                     /locus_tag="ACHLAG_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        12993 a        12949 c        12541 g        12529 t
ORIGIN      
        1 aggagagggt tatgacgaat atcaccttac agaaacaaca tcgtacactg tggcatttta
       61 ttccggggtt agccctgagt gcagttatca ccggggtcgc cctgtggggt ggttccattc
      121 ccgcggttgc gggtgccggg tttagtgccc tcaccctcgc aatcttgttg gggatggttt
      181 taggcaacac catctatccg cacatctgga aaagctgtga cggtggtgtg ctgtttgcca
      241 agcaatattt attacgtctg ggtatcattc tttatggctt ccgtctgacg ttctcgcaaa
      301 ttgccgatgt cggtatcagt gggatcatca ttgacgtgtt gacgctgtcc agtaccttcc
      361 tgctggcttg cttcctgggg cagaaagtgt ttggtctgga taagcacacc agctggttga
      421 tcggtgccgg tagcagtatc tgtggtgctg ccgcggtact ggcgactgag ccggtagtga
      481 aagcggaagc cagtaaagta accgtggctg ttgcaaccgt tgttatcttc gggaccgtcg
      541 cgattttcct ctacccggcg atatatccgc tgatgtctca atggtttagt ccggaaactt
      601 tcggtatcta cattggttct actgtgcacg aagtggcgca ggtggtggcg gtaggtcatg
      661 ccatcagccc ggatgcggaa aacgcagcag ttatttccaa aatgctgcgc gtgatgatgc
      721 tggctccgtt cctcatcctg ctggcggcgc gtgttaaaca gctgtctggg gcgaacagcg
      781 gcgagaaaag caaaatcact attccgtggt ttgccatctt gttcatcgta gttgccatct
      841 ttaactcgtt ccacctgtta ccgcagagcg tggtgaacat gctggtaacg ctggatacct
      901 tcctgctggc aatggcgatg gcggcgctgg gtctgaccac tcacgtcagc gcgctgaaaa
      961 aagccggggc gaaacctctg ctgatggcac tggtactgtt tgcctggctg attgttggtg
     1021 gtggtgcgat taactatgtg attcaaagcg tcatcgcata aaccactaca tcttgctcct
     1081 gttaacccgc tatcattacc gttttcctcc agcgggttta acaggagtcc tcgcatgaaa
     1141 tacattggag cgcacgttag tgctgctggc ggtctggcaa atgccgcaat tcgcgccgcc
     1201 gaaatcgacg caaccgcgtt tgccttgttc accaaaaacc aacgtcagtg gcgtgccgca
     1261 ccgctcacga cgcaaaccat cgatgaattc aaagcggcct gtgaaaaata tcactacaca
     1321 tcggcgcaaa ttcttcccca cgacagctat ctgattaacc tcggacatcc ggtcactgaa
     1381 gctctggaaa aatcgcgcga tgcctttata gatgaaatgc agcgttgcga acaactgggg
     1441 ctttctttgc tcaacttcca ccctggcagc catctgatgc agatttcaga agaggattgc
     1501 cttgcgcgta ttgccgaatc catcaacatt gcgctggata aaactcaagg tgtaacagcg
     1561 gtgattgaaa acaccgccgg tcagggcagt aacttagggt ttaaattcga acatctcgcg
     1621 gcgattatcg acggcgtgga agataaatcc cgtgtcggcg tctgcattga tacctgccat
     1681 gctttcgctg ccgggtatga tttgcgtact ccagccgaat gcgaaaaaac gttcgctgat
     1741 tttgcccgta ttgtcggctt taagtatctg cgcgggatgc accttaacga tgcgaaaagt
     1801 acctttggca gccgcgttga ccgccatcat agcctcggtg aaggcaatat cggtcatgat
     1861 gcgttccgct ggatcatgca agacgaccgt ttcgacggca ttccgctgat ccttgaaacc
     1921 atcaacccgg atatctgggc agaagagatc gcctggctga aagcgcaaca aactgaaaaa
     1981 gcggtggcct gatcgatgaa cgacagggaa aagcagattc ttaaaatttt acggcgtaac
     2041 ccgctgattc aacagaatga aatcgccgat attctgcaaa tcagccgctc acgtgttgcc
     2101 gctcatatta tggatttgat gcgcaagggt ttgattaaag gcaaaggata tatccttacc
     2161 gagcaggatt actgtgtggt tgtgggggca atcaatatgg atattcgcgg aatggcggat
     2221 atccgttacc cgcaagcagc ctctcatcca ggttctgttc actgttccgc aggcggcgtc
     2281 ggacgtaata tcgcgcataa cctggctctg cttggtcggg atgtgcattt aatatcagct
     2341 attggcaacg atttttacgg cgagacatta ctggaagaaa cgcgccgcgc gggcgtgaac
     2401 gtttcaaact gcattcgtct gcatggacac agcactgcaa cttatctcgc tatcgctaat
     2461 aagcaggaag aaactatcct ggcaatcaat gacacacata ttctgcaaca gctaacgcct
     2521 caactgctca atacttcacg ggacttgatt cgccatgctg gtgtggtgct ggctgattgt
     2581 aatctcacac ctgaagccct ggagtgggtc tttaccattg ctgatgaaat tccgatgttt
     2641 gtcgataccg tttccgaatt taaagcaaac aaagtaaaaa actggtacag ccggatccac
     2701 actctgaaac caacacaaaa tgaactggaa attttgtggg ggcaaccgat taaagacgac
     2761 aacgatcgca tcagagctgt aaattcgctg catcaacaag gtgttaaacg catttttgtt
     2821 tatctgaaag atgaatctgt attttgcagc gataaggatg gagaacaatt tttgctgacc
     2881 gcgccagcgc atacgacagt agacagtttt ggtgctgacg atggttttat ggcgggcctg
     2941 gtatatagct ttcttgaagg atacagtttc cgcgacagcg cccgttttgc ggtagcctgc
     3001 gcggcaattt cgcgcgccag cggcagctta aacaacccta ccctgtctgc cgataacgcg
     3061 ctttcattag tgccaatggt gtaacaatgt tgccggattc gacgcttgac cgcatcttat
     3121 ccggctcata aattgcgcac tacgctaaac caataaagaa tcctgcaata gtagcactca
     3181 tcaggttaga aagtgtcgcc gccgccagcg cgcgtaaacc aagttgggcg atttccggcg
     3241 cacggtgtgg cgcaaccgca gaaaacgccc ccaccaccac cccgatagaa ccaaagttag
     3301 cgaaaccgca caacgcgaaa gaaataatcg ccacggtttt agcatccaga gtgccacccg
     3361 tttgcagata tggcgagaaa ttgagataag cgacaaattc attgatcgcc agcttctgcc
     3421 caatcaaact cccggcaaga tttgcatcac tccagtcaac ccccatcacc cacgccaatg
     3481 gggccaacag gtaacctaaa atggactcca gcgaggcatg tgcaaaacca aaccagccgc
     3541 caacgccgcc gataatacca ttaattaacg caatgatggc gacaaatgcc ataacaactg
     3601 tcgctacacc ggcggcgatt ttcagcccgg tcattgcccc tgtcgcagcg gcttcaataa
     3661 tgctttttgg cggtgtttcg gtgaaagaga ggttattaaa ggaaacctgc gaagattccg
     3721 tagccgggct taacaggcgg gcaaacaaga tcccgcctgg gatcgccatt aacgatgccg
     3781 ccagcaaata ttccacaggt acgcccaggg cggcgtaacc aatcattgtc gaaccagcaa
     3841 tcgaggccat gccactacaa atcgctgtaa ataattcatt gcgattcaga cgatcgataa
     3901 agggtttgac gatcgccgga atttcgtttt gcccgaggaa aatggtggtg accgcgacga
     3961 atgactcaat cttgctgata tttaatgctt tctggaatat accgccgaga atgcgaatta
     4021 aaatccccat cacaccgatg tagtagagaa tactcaccag tgcagtgacg aagataattg
     4081 ccggtaatac cctgaaacca aagataaatc ctgcgccatc aaacagcgtg tccattttcg
     4141 gcccgaccag cgaaccgaag ataaatgcgc tacccgcgtc gctgtacgcc atcactttat
     4201 gcacgccaaa agcgactttt tcagcgaccc aacgccctgg cggtaaccaa agcataatcc
     4261 cgccaatcac gacctgtaac actaacgccg cgccaacggt acgcaggctg atcttcttct
     4321 tgtttactga cagcaaaaac gcaatcgtca gcaataccac cattcccaga acacttctca
     4381 tgacatccat agttattatc tcttcatgtc atattgggcg acggctttct gcccatcaga
     4441 aatagccggg cttggatctt gcgatactga cccggcttgt tatcaacact taatgggttt
     4501 tgatgtagcc gcgcacgcac tcttcgacta agccccagaa ccagtcggta tcaatagtga
     4561 tgccgacttt ggtattagcg ggcttgccaa gaacgcccag ctcgtcgcag acggtacgcc
     4621 catagcaagg gccactgttg acgtccactt cgacgtacat ctcctgtgtt ttaatgccat
     4681 cagggttaat cagataaccg atgcaggtgg cgtcgtgcac cgggccgcca gccaggccgt
     4741 agttttcgaa ctgcgttttg agagtgaagt tcatgatgtc gctgaacagc tctccggcag
     4801 ggccgcctgc cctttccatc cgtgcaatca cgtccggggt gcaaacggtc tggttggtga
     4861 gatcgaggcc catcatcact aatggaacgc cggaggtgaa caccacgcgg gctgcttccg
     4921 gatcagcaaa gatgttgaac tcggcagatg gcgtgaagtt gcctgtaccg taagcgccgc
     4981 ccattagcac aatttcacgg attttgggca ggatcgcggg ttgcatacgc attgccaccg
     5041 cgatatttga aagcggacca accggcacca gagtgatatc gccatcgctt gccatcaggg
     5101 tatcaatgat gtatttcacc gcgtgggtac tttctgcctg gcgggtcagc ggctcgaata
     5161 ccgggccatc cagtccggtt tcaccgtgaa tattatcggc aacgatttgt ttacgcataa
     5221 tgggttgcgg catccccgca taaaccggaa cattaatctc cagtttctgg caaacattca
     5281 ggccattaat taatgtttta tcaagcgtct gattacccgc tacaatagtg atgcctaata
     5341 aatctattgc cggatgtttc gctgccatca tcattgcaat agcatcatca tgacccggat
     5401 cacaatccag aataattttt ctcttttcca ttgtttattt cctctgtttc cagttgcgtt
     5461 attttttcta cagcaaagaa aattcgaccg ggccgatgat tgaatcttac caaccgcgta
     5521 cgtatgctaa atatgagaaa tctcatagcg gataaacatc gtgaaagaaa tccacaataa
     5581 tgatcttaag cagcaattga tgagtgaatc cgcgtttaag gattgctttt taacggatgt
     5641 ttcagccgat acgcggctgt ttcatttttt agcgcgtgac tacattgtgc aggaagggca
     5701 acagccgtcc tggctgtttt acctgacgcg aggccgcgcc aggctttacg ccacgctagc
     5761 taatggtcgc gtgtcgctga tcgatttctt tgccgccccc tgttttattg gcgagattga
     5821 gttaatcgac aaagaccatg aaccgcgtgc ggtgcaggct attgaagagt gttggtgcct
     5881 tgcgctccct atgaaacatt accgcccgct gttattaaac gacacgctat ttttacgaaa
     5941 actctgcgtc accttaagtc ataaaaatta tcgtaatatt gtttctttaa ctcagaatca
     6001 atcatttccg ttagttaatc gcctggcagc ttttatatta ctctcgcagg aaggtgatct
     6061 ttatcacgaa aagcatacgc aagcggcaga gtatttaggc gtttcttatc gacatctttt
     6121 atatgttctc gcgcaattca ttcacgacgg tttattaact aaaagcaaga aagggtatct
     6181 cattaaaaac agaaagcagt tgtcaggact ggcgctggag atggacccgg agaataaatt
     6241 ctccgggatg atgcagtaaa aattatttgc aatagcgcga ttgccggatg caacgcttaa
     6301 tacgttttat ccggtctaca aatcgagcat tacgccagac caataaagaa cccggcaata
     6361 gtcgcactca tcaggttgga aagcgttgct gctgccagcg cccgtaaacc aagctgggcg
     6421 atttctggag cacgttttgg cgaaatagcc gaaaatgcgc caacgacaac accgatagaa
     6481 ccaaagtttg caaacccaca gagggcgaac gagataatgg caatggtctt gacatccagc
     6541 gtgccgctcg tttgcagata gggcgagaaa ttgagataag caacaaactc attaatcgcc
     6601 agtttctgcc caatcaaact tccggcaaga tttgcatcac tccagtccac ccccataatc
     6661 catgccaacg gggccagcac atagccgaaa atgctttcca gagagacatt ggcaaaacca
     6721 aaccagccgc caactccacc aataatgccg ttgatcagcg caataattgc cacaaatgcc
     6781 attaccaccg tcgccacacc ggcggcgatt ttcagcccgg tcattgcacc gttcgccgca
     6841 gcttcaataa tgctttttgg cggtgtttcg ctaaacgaca gattttcaaa cgtaacttgc
     6901 gaaggttccg tcgccggact aagaatacgt gcaaacagga taccgccagg aatagccatc
     6961 agcgaagcgg ctaacaaata gtcaatcggc acgcccattc cggcatagcc aatcatcatc
     7021 gaacccgcaa tagacgccat gccgctacaa atagccgtaa atagctcgtt gcgattcatg
     7081 cgatcaataa aaggtttaac gatcgccggg atctcatttt gcccgaggaa aatagtggtg
     7141 actgcgacaa aagattcgat tttgctgatg ttgagggctt tctgaaaaat actgccaaga
     7201 atacgaatca gcagccccat tacgccaatg tagtacagca agctgataag cgcggtaacg
     7261 aaaataatcg ccgggagtac gcgaaaggcg aagataaagc ccgcaccgtc aaacagaaca
     7321 tccattttgg gaccgaccag cgaaccaaaa ataaaggcac ttcctgcgtc actgtaggac
     7381 atcactttat ggacgcctaa tgccgcctgc tctactgccc attttcccgg gggaaaatag
     7441 agcatgatgc caccaatggc aatttgcagc agcagcgcag cgccaaccgt gcgcaagctg
     7501 atgctctttt tatttaccga caacacaaag gcaattgcca gcaataccac catccccaca
     7561 acacttctca ttatatccat aatgattttc ccttcatgcc agtaaacccg gcgtcagcgc
     7621 caggttttgg tatgtttgat gagtacgggc gacggctttc tgcccgccag aaattaaccc
     7681 acgagacgct gatattcttt ggcaatttcg ctcgccagaa tggcgttatt aaagaccagt
     7741 tgaatattgg atttcaggct atcaccgcct gttagctccg caacacgcgc cagcaggaac
     7801 ggtgtactct ctttcccaat aacaccctgt tcttcggctt cagcaacggc ttgatcaatt
     7861 gctgcattga tggtttcttc tggcatcgca aactgttccg ggatcggatt cgccactacc
     7921 aggccgccgt tcaaaccggt ttgccacttc actgccatcg cgcgagcaat ttcgcgggca
     7981 ctgtcaagac gaatgctgac ttcaaatggg ctggttcggc agaaaaacgc aggcagcgct
     8041 tttgtctgat aaccaattaa cggtacaccg aaggtttcta aatattctgt tgttaatcct
     8101 aaatcgagaa tagattttgc ccccgcacaa acaacagtga cattggtatt tgccagttcc
     8161 tgtaaatcgg cagaaatatc gaaggtatgt tccgccccgc gatgcacacc accaattccc
     8221 ccggtggcaa atactttaat tccggcaagc gccgcaataa tcatcgttga agccacagtg
     8281 gttgcgccat tttttccggc ggcaacaaca aaaggtaaat cgcgacgact aactttggtc
     8341 acgttatgtc cttcacggcc cagcaattcg atttcttctt tacttaagcc cactttcatc
     8401 acaccgccaa taatggcgat agtggctggt actgcgccct gtttacgaat agtttcttct
     8461 acttcaattg cggtttgggc attttgtggg aatggcattc cgtgagaaat tatggtcgat
     8521 tccagcgcaa caaccggttt tttatttttt aaagcatcct gtacttcagc ggatatttgt
     8581 aataattcag gggacagagt taattcagac attctgtgtt ctccactaac gatctaacat
     8641 tggcaattga taattcaggg ttgttggtat attcacaggc aagtgccatt gacgaacatc
     8701 cctgcgcaaa tcgaacagaa tcgataaacg gcataccgtc tacccaacag gaggccagcc
     8761 ccgccatcat ggcgtcgcct gccccagtaa cattgatgac attcgtttta ataggggccg
     8821 accacccact ttcaccgttg atatcactgt agtaaacgcc atcccctccc atgctcagca
     8881 ccaggcggtt caggccgtgt tgatgaaacc aggcggcgac ctttgcgaca tcttcacgtc
     8941 cggaaagtgc aattccgctc aacgtttcgg cttcaagacg gttaggtttt aacgtatgga
     9001 ttttgctcag atgatcgcgg attttgacgc atttccatgc cgataccgga tcaataaata
     9061 caggcgtttc cccgctattt tccagaaccc agaccagcgc ctcttcacta aggttgcaat
     9121 ccgcgacaat aacctttgca ccgcgaataa actcctgatg ctgggcaaga aattctgcag
     9181 aaatacagtc actaatactc atatcattta tcgcgacaag catttctcct gtattatcga
     9241 gcagtgataa atagctggat gtattctcac ccggaacaat taagcattta tcgacataaa
     9301 caccggattg attagtttgc gccagtaatg attgcccata aaaatcacca cctactgcac
     9361 ttaaaagcca ggcatttttc cccagtaacg ccagattatg agcgatattc cttcccacgc
     9421 cacctggggt aaatttaatt tttccgggat tcgaatccgc ataattcaat ggatgatgtg
     9481 aatacccagc cacatcaata ttcgccgaac caataattac tatgtagtcc ttttcgcgca
     9541 tagcacgccc cctggcaata aattgccaac gaagaaattg ggtaaaatag tcattacagt
     9601 caataagact attagagcac gtgttcatat ttaaacacat gttcataata agcatattta
     9661 acgtaatgta aatcgatcag aaaaaacgaa atcggccaga tatgaaggcg gtcacaaagt
     9721 ttgtctattt atgcagcaac aaaagagaaa gttgtgatgt gtaacacaat atggcaggca
     9781 agaaaaaggg cagagatgtt tctgccctcc agattgttga caacgcgcgc tttgttcatc
     9841 ctggatgtgg cgtaaacgcc ttatccgacc tacaaaagcg caaaaattca atatattgca
     9901 gggttcatgt aggtctgata agcatagagc atcaggcaat tttgcgtttg tcatcatcgg
     9961 ggcagagatg tctcatcccc gataatgaag ataaattatg cgtttttttc tactatttgt
    10021 gtttccgggc gtttcaggaa ggcataggcc agacccgcca ccagcgtacc ggcaataatg
    10081 gctaccaggt aacccagtac cggcgtaatc gcgccaggga tcagcagaac aaacagacca
    10141 ccgtgcggtg ccatcagttt cgcaccaatc gccattgaga ttgcgccagt cagcgcccca
    10201 cccacgatac agcacggcag cacacgcatc ggatcacgag cagcaaacgg aattgcacat
    10261 tccgaaatga agcacagtcc caataccaga gcggctttgc caccttcctg ctgcgctttg
    10321 tcgaatttgc gacgcgccac cattgttgcc agacccattg ccagcggtgg caccatacct
    10381 gccgccataa tcgccgccat cgggccatag gtttgagtac tcagcagacc cacaccgaac
    10441 gcgtacgctg ctttatttac cggaccgccc atgtcagtac acatcatgcc accgaggatc
    10501 gcccccagca gaaccgcatt cgcagtcccc atggtctgca gccagtgagt cagcccttcg
    10561 agaatgccag caactggttt accgatcagg tagatcatcg ccagaccgac caccagactg
    10621 gaaattagcg gaatgatcag gattggtttc agcgcctcca tactctgtgg cagtttcagt
    10681 tgcgtactga ttaacttcgc aatgtaacca gccaggaagc ccgcaataat accgccaatg
    10741 aagccagaac cggtgctgac agccagcata ccgccaatca gacccggcgt gaggcccgga
    10801 cgatcggcaa tggagaaggc aatataacct gccagtaccg gcaccatcag cgcaaaggct
    10861 gaaccaccgc caatctgcat cagcgccgct gccaacgtac ccggctcttt aaacgcttcg
    10921 ataccaaaag caaaagaaag cgcgatacac agaccaccag caacgaccat cggcagcatg
    10981 taagaaacgc ccgtcagcaa gtgacggtaa gcgcccgcac tctctttctt accttcagtg
    11041 gtcgccgttt gagctttgcc cgccggttca tacggcgttg cttcagcaac cgctttatcc
    11101 agttcctgcg cggttttctt cagcgccaga ccggtagagg tgcgatacat cggtttacca
    11161 gcaaatttcg ccagatccac ttcgatatct gccgccacaa tcaccagatc cgctgcggct
    11221 acttcttcgg gagtgattgc attacccgcg ccaacagaac cacgggtttc aactttcacc
    11281 caccagccac gttttttcgc ttcggtttca atggcttcag cagccataaa ggtgtgtgct
    11341 acgccagtcg ggcaagcagt caccgcaact acgcgtttcg ggccgctggc gtcaaccggt
    11401 gctgtcgcag cgaccggcgc agtgtaaggt ttcgcatggc ctttggcttc actcaggaac
    11461 agctcagggt gcgcaactgc ccgggaaata tcgccctgcc agacattttt accgttcagc
    11521 gcgctgtcgt tcgggatgga atcaccgaga acaatcgcca tttcagcgtc gttcggattg
    11581 tcgatgattt ccagttttgc ttttcgcgcc gccgcgccca gcagggtctt cgccatatag
    11641 gcgcgtgcct gaccgagatt agcgtcaata atcagcagcg ttttcattat gcctctcctg
    11701 ctgtcagtta aaaggttgta agtcgacgcg cgccatcatt gcggccaact gcggacgatc
    11761 ggtaataccc acattgcttt gacttaccgc cagggctgca acagcagtcg ccagacgcag
    11821 tgtgtgttca ctggattcac gcatcagcaa gccataaatc aggccaccaa ccatagaatc
    11881 ccctgcgcca acggtgctta cgacatcgac tgacggtggt ttggcgatcc attcgccgga
    11941 ggcattaacc caaagcgcgc cttcggcacc cagtgaaata acaacatgcg caataccttg
    12001 ttcacgtagc gcatgtgcag cttcaatcac atctttcatt tcaggcagtt tacggcctgc
    12061 ccagatttcc agctcgcggc ggttaggttt caccagccac ggtgccgctt tcaaacctgc
    12121 tactaacgct tcacggctac tatcaaagat aatgcaagga cactgactac gcaggcgagt
    12181 catccagtcg gtgaacgctt ccgggctgac gcctgacggt aagcttccgc tgacacagac
    12241 catatcgaac tgaccgagcc agctcagaga atcagtcaca aagcgttccc agtcggcggg
    12301 ggtgacttca aaacccgaga agttgaagtc ggtcacttcg ccgtcttttt ccgtcagctt
    12361 aacgttaatt cgggtgcgcc cctgtacaac ctggaaacgg ttggcaatgc ccagctcgct
    12421 gaacagttgc tgaaaaccat cctgattgtc tttaccgagg aagccgccaa cggtgacatc
    12481 aattcccagg tcttttaata ctttggccac gttgatgcct ttacccgccg catgcagacc
    12541 ggtggttttc accaggttca cttcgccgcg ttcaatttcc gggcagaaac caacaaggtc
    12601 ataagccgga ttaagggtga tcgtagcaac acgtctgctc attatgcgcc ctccccaaga
    12661 ccagcagcga tagcgtcgcc aatggctttc agcgcctgtt cagcatctgc accctgggcg
    12721 gtaaagcgta ggcgatgacc tttcttaacg ccaagtgcca caactttcat cagactacgt
    12781 ccgtttgccg gtttgccggt gccatcaagg tttgtcacgg taatatcact gttaaattgt
    12841 ttaatggtat tgaccagcat ggtacctgga cgagcatgca ggccgtgttc attgcgcacc
    12901 acaaactccg cgcttaacac gtcgtcggtc ggcgcatcat cgctggtcag cagcgccagc
    12961 aacgttgccg catccgcttt cagcaagcga tcagctttat tgtcgagcaa caaatcagcg
    13021 agacgcttaa gaaccgcgat gggctgatcg tcattcatcg ccacactcac cagcatggct
    13081 gccgtttcgc cgtccacatc aaaagcattt gccgcacggc ttaccgcaat cgcgctacgc
    13141 agattgcctt cggcgctatc gctcagccag ataccctgtc cgaggttcag cggttgttca
    13201 ttgatggctt tggtgacgaa agtggcgtca actgcccccg cctctttcag acgcgcagcg
    13261 ttcagcgcct gaagagtcag cagatcgctg gcgacgatat ccagcgtcag catttcgttg
    13321 tcgagcttca gctgctcact ctgcttttcg cccatcagta atgcgcgaag ttcttctgct
    13381 gttgttgctg acttcagttg ttcagcaacg gaatcatcgc tcagtacgtg ggtcagctgg
    13441 cgtagcaggc ccagatgttc atccgagctg gcagcaatac cgattgccac gtacgctacc
    13501 tgaccgtcac cccaggtgac gccttccggg aactgaaata cctgaacgcc ggttttcagc
    13561 acctgatcgc gggtgtcggt agtgccgtgt ggaatagcaa taccattgcc gaggaacgtt
    13621 gaggtttgct gttcgcgcgc cagcatgcca ttgacgtagc cttctgctac attaccggcc
    13681 tgcaccagcg ccgcagcgac ctggcgaatc gcctcttctt tgtctccggc cttttcgccc
    13741 ggatggatgt cctgtacgga taactggaac atagttctcc tctcttgctg aattgaaacg
    13801 attcagcctc tatgagaaaa aaagcgccaa cctggcttag ggttaaagac aagatcgcgc
    13861 tgaaacgttt caagaaagca taatacttct gtttcagcac gcaaggaaag ccgtaaagtt
    13921 atgagcaaaa gtttgatgtg ctgcacaatt ttttgccatt tttcctaatt gctgatggga
    13981 aaatccgtta tcagcaattt catttcagca gcgtcagcag ccaatcagca acagtgaaat
    14041 ggcattttga ttattcgtgg cgcttttgat ttcgctgtct gtttattttc tgacaagcag
    14101 cgtaaactcc gcgtcttcct cttccagtga tcgaccagca tgcataactc ccccgcagtc
    14161 tccagcgcga aatcgtttga cctgacctcg acggcgtttt taatcgttgc ctttctcacc
    14221 ggtattgcgg gcgctctgca aaccccgaca ctcagtattt ttcttactga tgaagtacat
    14281 gctcgtccgg cgatggtggg attcttcttt accggcagcg ctgtcattgg gattctggta
    14341 agtcagtttc tcgccgggcg ctctgataag cgcggcgatc gcaaatcgct gattgtcttt
    14401 tgctgcctgt taggcgtgct ggcctgcacc ctttttgcct ggaatcgcaa ctactttgtt
    14461 ttgctattcg ttggcgtctt tcttagcagc tttggctcga ccgctaaccc gcaaatgttt
    14521 gcccttgccc gtgaacatgc cgacaaaacc ggacgtgagg cggtgatgtt cagctctttt
    14581 ttacgcgctc aggtttcact ggcatgggtc attggcccac cgctggctta tgccttagcg
    14641 atgggtttca gctttacggt aatgtatctg agcgcagcgg tagcgtttat tgtttgcggt
    14701 gtgatggtgt ggctgttttt accgtcgatg caaaaagagc ttccgctggc gaccggcacg
    14761 atcgaagcgc cgcgccgtaa ccgtcgcgat acgctgctgc tgtttgtcat ttgtacattg
    14821 atgtggggct cgaacagcct gtacatcatc aacatgccgc tatttattat caacgaactg
    14881 catcttcccg agaaactggc cggtgtgatg atggggaccg ccgccgggct ggaaatcccg
    14941 acgatgttga ttgccggata tttcgccaaa cgtctgggta agcgtttctt aatgcgcgtt
    15001 gctgccgtgg gtggcgtctg tttttacgca ggaatgctga tggcgcattc acctgtcatt
    15061 ctgttgggct tgcagctgct aaatgctatt tttattggca ttctgggcgg catcgggatg
    15121 ctctattttc aggatctgat gcccggtcag gcgggttcag ccaccacgct ctataccaac
    15181 acgtcgcgcg tgggctggat catcgcagga tcagtggcgg gcatcgtcgc cgagatctgg
    15241 aattatcacg ctgtgttctg gtttgcgatg gtgatgatta tcgccactct gttttgctta
    15301 ctgcggatta aagatgttta aggcgcggtc agcatttcca gatcgataag ccaggtcatg
    15361 gcctgctgac gcgagttacc gcacatttca gcgctggctt gtaagcctgc acagactttc
    15421 ggtcgcagtg gcgaggtgaa aattttacag cgttgctgtt catcaagctg aatgcagggc
    15481 gtattggcgg gcttgccatc tggcataccg ggaatagggc tggaaattga aggggcagtg
    15541 caacacgccc cgcaacccgg acggcatttc atacgttgtt ctcttaaggt taaccaccgg
    15601 attgatgagc gcgcacagta ccaccttttt tgcaccagca aaagtgcgaa taccacttgc
    15661 ccgaaaggcc cgtcgcgagt actttgtcgc gatatttttg acattttcga ctacaggaat
    15721 ttttcgatgc caagagcgaa cgaaattaaa aaaggtatgg tactgaatta caacggcaaa
    15781 ctgctgctgg ttaaggatat tgatattcag tcgcccactg cccgcggtgc cgctacgctg
    15841 tacaaaatgc gtttttctga tgtccgtacc gggctgaaag tagaagagcg tttcaaaggt
    15901 gatgatatcg ttgacaccgt gacgctgacc cgccgttacg ttgacttctc ctatgtcgat
    15961 ggcaacgaat atgtctttat ggataaagaa gactataccc cgtatacctt caccaaagat
    16021 cagattgaag aagagttgct gtttatgcca gagggcggca tgccggacat gcaggtgctg
    16081 acctgggacg gtcaactgct ggcgcttgag ctgccgcaga ccgttgatct ggaaatcgtt
    16141 gaaaccgcac caggcatcaa aggggcatct gccagcgccc gtaacaaacc ggcgacattg
    16201 agcactggtc tggtgattca ggtaccagaa tacttaagcc cgggcgaaaa aattcgtatc
    16261 catatcgaag aacgccgtta tatggggcgt gctgactaac ttcagccgca tgcagaaaag
    16321 ggatagctca ggctgtccct tttttaattt attataccaa tcttctattt tgcgcttcct
    16381 gaacacctta cgccctcaat tttcactcgt tgattgatcg ccctcacact tcatcgcatt
    16441 aacaatccag accaatttca attgctgtca tataacttta cactgtcatt gttaatttat
    16501 cgttactaag acgtgactcc tatgaagaca attgcctccg ttacgctccc gcatcatgta
    16561 cacgctccac gctacgatcg ccagcagttg caatcacgta tcgttcattt tggctttggc
    16621 gcctttcacc gcgctcatca ggcgttactg accgatcgtg tgctgaatgc ccagggcggc
    16681 gactggggga tctgtgaaat cagcttgttc agcggtgatc aactgatgag ccagctccgc
    16741 gcacagaacc atttatatac cgtgctggaa aaaggtgcgg acggcaatca ggtgataatt
    16801 gtcggtgccg ttcacgaatg ccttaatgca aaactggatt ccttagcggc aattattgag
    16861 aaattttgcg agccacaggt ggcaattgtt tccctgacga ttaccgaaaa aggctattgt
    16921 attgacccgg ccaccggtgc actcgacacc agtaatccgc ggattattca cgatctacaa
    16981 acccctgaag aacctcactc cgcaccgggt attctcgtcg aagcactgaa acgccgccgt
    17041 gagcgcggcc ttacaccgtt taccgtgctc tcctgcgaca atattcccga caatggccat
    17101 gtggtgaaaa acgcggtgct gggaatggca gaaaaacgtt cgccagaact cgccgggtgg
    17161 ataaaagaac acgtcagttt tccgggaacc atggtcgacc gcattgttcc ggctgcaacc
    17221 gacgaatcac tggcagaaat cagccagcat ctgggggtaa atgatccctg cgcgattagc
    17281 tgcgaaccgt ttatccagtg ggtggtggaa gataacttcg tcgctgggcg tcctgcctgg
    17341 gaagtcgcag gtgtacaaat ggtgaatgat gtcctgccat gggaagagat gaaactgcgt
    17401 atgcttaatg gcagccactc ttttctcgct tatctgggtt acctttcagg attcgcccat
    17461 atcagtgatt gcatgcagga tcgcgcattt cgccatgccg ccagaacatt aatgctgaat
    17521 gagcaagcgc cgacactaca aattaaagat gtcgatttaa cacaatatgc ggacaagtta
    17581 attgcacgtt ttgctaatcc ggcgctgaaa cataagacct ggcaaatcgc aatggatggt
    17641 agccagaaat taccgcaacg tatgctggca ggtattcgca tacatctggg gtgcgaaacg
    17701 gactggtcgt tgctggcatt aggcgttgct ggctggatgc gttacgtcag cggcgttgat
    17761 gatgccggaa atgccattga tgttcgcgat ccgcttagcg ataaaattcg cgaacttgtt
    17821 gcgggcagca gcagtgaaca acgcgtaacc gccctgcttt ccctgcgtga agttttcggt
    17881 gatgatctgc cagataaccc gcattttgtg caggccatcg aacaagcctg gcaacaaatc
    17941 gtacaattcg gcgcacatca ggcgctatta aacaccctca aaatttaacg atttctgcgg
    18001 ttaaagcgga tgaagctcac cttcgtccgc tctccccttc tcttttctgc cttttttagc
    18061 caggattaac gctcagttaa cttaccagag tattgcggag ccgttgtgac caggaccaac
    18121 ctcatcaccg gttttctcgg cagcgggaaa accacgtcga ttcttcatct gttagcccat
    18181 aaagatccca acgaaaaatg ggcggtactg gttaatgaat ttggggaagt cggaattgat
    18241 ggtgctttgc tcgccgatag cggcgcattg ctgaaagaga tccccggcgg ctgcatgtgc
    18301 tgcgttaatg gtttacccat gcaggtaggg ttgaatacct tactgcgtca gggaaaacca
    18361 gaccgcttgt tgatagagcc gaccgggctg ggccatccga aacagatcct cgatctgtta
    18421 accgcaccag tctatgaacc gtggatagat ctgcgcgcca ccttgtgcat tctcgatcca
    18481 cgcctgctgc tggacgaaaa aagcgccagc aatgaaaact tccgtgacca gctggctgcc
    18541 gcagacatca ttgtcgccaa taaatccgac cgtgcgacgc ccgaaagtga gcaagcgcta
    18601 cagcgttggt ggcagcaaaa tggtggcgat cgacaattaa ttcacagtga gcatgggaaa
    18661 gttgacggtc atcttctgga tttgccgcgt cgcaatttag ccgagttgcc cgccagcgcc
    18721 gcgcattctc atcagcatgt cgtgaaaaaa gggttagcag cgttaagcct gccagagcat
    18781 caacgctggc gtcgcagtct gaacagcggg caaggatatc aggcctgcgg ctggatattc
    18841 gacgctgata cggtattcga caccattggc attctggaat gggcgcgact tgcaccggtg
    18901 gaacgcgtca aaggcgtgct gcgtattccc gaagggctgg tgcgaatcaa ccgtcagggc
    18961 gatgacctgc acattgaaac gcaaaacgtt gcgccaccgg acagtcgtat tgagctgatt
    19021 tccagcagcg aagctgactg gaatgctttg cagagcacgc tgttgaagct tcgtttagcg
    19081 actaccgcgt aaggttgcct gcgtttttca gtaagataat tagagaaaat atgattaaaa
    19141 atttgccgca aatagtgttg ttgaatattg tcggcctcgc gctgtttctt tcctggtata
    19201 ttcccgttaa tcatggattc tggttgccga ttgatgcgga tattttttat ttctttaatc
    19261 agaaactggt cgaaagtaag gcctttttgt ggctggttgc attgaccaac aatcgcgcct
    19321 tcgacggttg ttcactgctg gcgatgggta tgttgatgct gagtttctgg ctgaaagaaa
    19381 acgccccagg cagacgacgt atcgtgatta tgggtctggt catgctatta actgcagtgg
    19441 tattaaacca gctgggtcag gcattaattc ctgtaaaacg ggccagccca acattgactt
    19501 ttaccgacat taaccgcgtc agcgaactgc tctctgttcc cacgaaagat gcctcacgag
    19561 atagctttcc cggcgatcac ggcatgatgc tgcttatttt ttcggcattc atgtggcgtt
    19621 atttcggcaa gttacaggtc ttatcgccct tattattttt gtggtttttg cgtttcccag
    19681 agtaatgatt ggcgcacact ggtttactga catcattgtc ggttcgatga ccgtgatatt
    19741 gatcggtttg ccctgggtgt tgctgacgcc attaagtgat cgattaatca ccttttttga
    19801 caaatcacta ccgggaaaaa acaaacattt ccaaaacaaa taactcacag taattaacat
    19861 catcaggttt atttttatag tgaggataat cctgatgatg cgcaccgtgc tttcatctat
    19921 cgaacgcaaa aatcattctc taagtaaatg aatggattgc atgcgtttca ctcaatcgta
    19981 ctttaattga ccaatcccgc ttattaactt tctgtatcac tttttcttat aaaaaatcat
    20041 gtaaaaccgc tcgccaagac cgcaccaatc gggtaatctc gaactcgttt tgcctcggcg
    20101 gtagattatc ctcacagcat ataattttgt gcgttagtcc acagatttgg ccttaaggaa
    20161 ttgtttcaac atgcccaggt aattagtctc gtgtcgcttg gcattttttt ataacgatat
    20221 ttgtcgttaa ggacttcaag ggaaaacaaa caacatggtc aaatctcaac cgattttgag
    20281 atatatcttg cgcgggattc ccgcgattgc agtagcggtt ctgctttctg catgtagtgc
    20341 aaataacacc gcaaagaata tgcatcctga gacacgtgca gtgggtagtg aaacatcatc
    20401 actgcaagct tctcaggatg aatttgaaaa cctggttcgt aatgtcgacg taaaatcgcg
    20461 aattatggat cagtatgctg actggaaagg cgtacgttat cgtctgggcg gcagcactaa
    20521 aaaaggtatc gattgttctg gtttcgtaca gcgtacattc cgtgagcaat ttggcttaga
    20581 acttccacgt tcgacttacg aacagcagga aatgggtaaa tctgtttccc gcagtaattt
    20641 gcgtacgggt gatttagttc tgttccgtgc cggttcaacg ggacgccatg tcggtattta
    20701 tatcggtaac aatcagtttg tccatgcttc caccagcagt ggtgttatta tttccagcat
    20761 gaatgaaccg tactggaaga agcgttacaa cgaagcacgc cgggttctca gccgcagcta
    20821 ataaaccgtt tggatgcaat cccttggcta tcctgacgag ttaactgaaa gcactgctta
    20881 ggcagtgctt ttttgttttc attcatcaga gaaaatgatg tttccgcgtc ttgatccagg
    20941 ctatagtccg gtcattgtta tcttttaaat gttgtcgtaa tttcaggaaa ttaacggaat
    21001 catgttcata cgcgctccca attctggacg taagctcctg cttacctgca ttgtagcagg
    21061 cgtgatgatt gcgatactgg ttagctgcct tcagttttta gtggcctggc ataagcacga
    21121 agtcaaatac gacacactga ttatcgacgt acaaaagtat ctcgatacct attttgccga
    21181 cctgaaatcc actactgacc ggcttcagcc gctgacctta gatacctgcc agcaggctaa
    21241 ccccgaactg accgctcgcg cggcgtttag catgaatgtc cgtacgtttg tgctggtgaa
    21301 agataaaaaa acattctgtt catctgcgac tggtgagatg gacattccac tcaatgaatt
    21361 gattccggcg ctcgacatta ataaaaacgt cgatatggcg atcttacccg gcacgccgat
    21421 ggtgccgaac aaacccgcaa tcgtcatctg gtatcgcaac cctttgctga aaaatagcgg
    21481 cgtctttgcc gctctgaatc tcaacctgac gccttcactc ttttatagtt cacggcagga
    21541 agattacgat ggcgtcgccc tcattattgg caatactgcg ctatctacct tttcttcacg
    21601 tttgatgaac gttaatgaat taaccgacat gccggtccgt gaaactaaaa ttgcgggcat
    21661 tcctctgacc gttcggcttt atgcagatga ctggacatgg aacgatgtgt ggtacgcatt
    21721 tttactgggc ggcatgagtg gaactgtcgt tggcctgctc tgctattacc tgatgagcgt
    21781 acgtatgcgc cccggcagag aaatcatgac cgccatcaag cgcgaacaat tttacgtggc
    21841 gtatcaaccg gtggtggata cacaagcttt acgagtcacg ggcctggaag tactgctacg
    21901 ctggcggcat cctgtcgcgg gagaaattcc cccggatgcc ttcattaact ttgccgaatc
    21961 gcaaaagatg attgtgccgc tgactcagca cctgtttgag ttaattgccc gcgatgccgc
    22021 agaattagaa aaagtactgc cggtaggcgt caaatttggc attaacattg cgccggacca
    22081 tctgcacagc gaaagcttta aagcagatat ccagaaactg ctcacttccc tgcccgcaca
    22141 ccatttccag attgtgctgg aaattaccga gcgcgatatg ctgaaagagc aagaagccac
    22201 acaactcttc gcctggctgc actcggtcgg cgtagaaatt gctattgatg acttcggcac
    22261 cgggcacagc gcgcttatct atcttgagcg ttttacgctc gattatctga aaattgaccg
    22321 tggatttatc aacgccatcg gtacggaaac gatcacttca cccgtacttg acgcggtgct
    22381 gacgctggcg aaacgtctca atatgctgac agttgctgaa ggggtcgaaa cgccggaaca
    22441 agcgcgatgg ctaagcgaac gcggcgttaa tttcatgcaa ggctactgga ttagtcgccc
    22501 gttaccgctg gacgattttg tccgctggct gaagaaaccg tatacgccgc agtggtaagg
    22561 tgtgcttacg tcccttatta ttcatagtga aagcatgccg gattgcggct aatgatgagt
    22621 aaaaggaaat ccgttgcaga tgattgtgcg catactgctg ctgtttatcg ctctgttcac
    22681 ctttggtgtg caggcgcagg ctatcaagga aagctatacc tttgccgtgc tgggcgaacc
    22741 ccggtacgcg tttaatttca accattttga ttatgtgaac cccgccgcgc caaaaggtgg
    22801 gcagataacg ttgtcagccc tcggcacctt cgataatttc aaccgctatg cactgcgcgg
    22861 caacccgggc gcacgcaccg agcagctgta cgacacgcta tttacgactt ccgatgacga
    22921 accaggcagt tattacccgc tgattgctga aagcgcacgc tatgctgacg attattcctg
    22981 ggtggaggtc gctattaatc cgcgcgcccg ttttcatgat ggttcgccca ttactgcccg
    23041 cgatgtagag tttacttttc aaaaatttat gaccgaaggc gtgccgcaat ttcgtctggt
    23101 ctacaaaggc accaccgtca aagccattgc gccgttaacc gtgcgcattg agttagctaa
    23161 acccggcaaa gaagatatgt tgagtctgtt ttcgctgccg gtatttccag aaaagtactg
    23221 gaaggatcac aaacttagcg acccgctcgc cacgcctccg cttgccagtg gtccgtaccg
    23281 cattacgtcc tggaaaatgg ggcaaaatat tgtctattcc cgcgtgaaag attactgggc
    23341 agcaaactta ccggtaaacc gtggacgctg gaatttcgac accattcgct acgattatta
    23401 cctcgatgat aatgtcgcct ttgaagcgtt taaagcaggt gcctttgatt tgcgtatgga
    23461 aaacgacgct aaaaactggg ccacgcgtta taccggtaaa aatttcgata aaaaatacat
    23521 catcaaagat gagcaaaaga acgaatcagc ccaggatacg cgctggctgg cgtttaatat
    23581 ccaacgtccg gtattcagcg atcgccgggt ccgggaagca atcaccctcg cctttgactt
    23641 tgaatggatg aacaaggcgt tgttttacaa tgcctggagt cgcacgaaca gttattttca
    23701 gaataccgaa tacgcggcca gaaattaccc cgacgccgcg gagctggtgc ttctggcacc
    23761 aatgaaaaaa gatctaccgc cagaagtctt cacacaaatc taccagccgc cggtatctaa
    23821 aggcgatggc tacgatcgtg acaacctgtt aaaagccgac aaacttctca acgaagcggg
    23881 ctgggtgctg aagggtcagc aacgcgttaa tgccacaacg ggtcagccac tcagctttga
    23941 attattgctt cccgcaagca gcaatagtca gtgggtattg ccgttccagc acagcctgca
    24001 acggctgggt atcaacatgg acattcgcaa ggtggataac tcgcaaatca ccaaccgcat
    24061 gcgcagtcgc gactatgaca tgatgccgcg cgtatggcgg gcgatgccgt ggcccagttc
    24121 cgatttacag atttcctggt catcggaata tatcaattcc acttataatg cccccggcgt
    24181 gcaaagtccg gttatcgact cgctgatcaa tcaaattatt gccgcgcagg gaaataaaga
    24241 aaaattactg ctgttagggc gagcactgga tcgcgtatta acgtggaatt attacatgct
    24301 gccaatgtgg tacatggcgg aagaccgtct cgcctggtgg gataaattct cccaacccgc
    24361 tgtacgccct gtttacagcc tgggtatcga tacctggtgg tatgacgtta ataaagcggc
    24421 caaactgccg tccgccagca aacagggaga gtagatgggc gcttacctga ttcgccgtct
    24481 gttgctggtg atcccaacat tatgggcgat tatcaccatc aactttttca tcgtgcaaat
    24541 tgcgcctggc ggtccggtcg accaggccat cgccgccatt gagtttggta atgccggagt
    24601 attacccggc gcaggcggtg aaggtgttcg tgccagccat gcgcaaacgg gtgtcggcaa
    24661 tatcagcgac agtaattacc gtggcggacg cggattagat ccagaagtga tcgctgagat
    24721 cactcatcgc tacggttttg ataagccgat ccacgaacgt tacttcaaaa tgctctggga
    24781 ctacatccgc tttgattttg gcgatagcct gtttcgcagc gcctcggtgc tgacgctgat
    24841 taaagacagt ctgccggttt ccatcaccct cggattgtgg agcacgctga ttatctatct
    24901 ggtgtcgatt ccgttaggca ttcgcaaagc tgtttataat gggagccgct ttgacgtctg
    24961 gagtagcgca tttatcatca tcggctacgc cattccggcc tttttgtttg ccatcctgct
    25021 gattgtcttc ttcgcgggcg gcagctattt cgacctgttc cctctacgcg gcctggtttc
    25081 cgctaacttt gattcgctgc cgtggtatca gaaaatcacc gattatctgt ggcatatcac
    25141 gctgccggtg ctggcgacag tgattggtgg ctttgcggcg ctgaccatgc tgaccaaaaa
    25201 ctcattcctt gatgaagtgc gcaagcaata cgtggtgacc gcgcgtgcga aaggggtaag
    25261 tgaaaaaaat attctctgga aacatgtgtt ccgcaacgcc atgctgctgg tgattgccgg
    25321 ttttccggcg acgtttatca gcatgttttt taccggctcg ctgctgattg aagtgatgtt
    25381 ttcactcaat ggccttggct tactgggcta cgaagcgacc gtctcgcgcg attatcctgt
    25441 aatgtttggt accttgtata ttttcaccct gattggcctg ctgctgaata ttgtcagtga
    25501 tatcagctat acgctggtcg atccgcgtat agattttgag ggacgttaat gtcgcgactc
    25561 agccccgtca atcaggcccg ttgggcgcgt tttcgccata accgtcgcgg ctactggtcg
    25621 ttatggattt tcctcgtttt gtttggtttg agtttgtgtt ctgaacttat cgccaacgat
    25681 aaaccgttgc tggtacgtta tgacggcagt tggtatttcc cgttgttgaa aaactacagc
    25741 gaaagcgatt ttggcggccc gctggcaagt caggctgatt atcaggaccc gtggctgaaa
    25801 caacggctgg aaaataacgg ctgggtactg tgggcaccga ttcgctttgg tgctaccagt
    25861 atcaactttg ctaccgataa gcccttccct tctccaccct cccggcaaaa ctggctggga
    25921 acggatgcca acggcggcga tgtgctggca cgcattctct atggcacgcg gatctcggtt
    25981 ctgtttggcc tgatgctgac tctctgttcc agcgtgatgg gcgtgctggc gggggcgcta
    26041 caaggctatt acggcggtaa agtcgatctc tggggacaac gctttattga agtatggtcg
    26101 gggatgccga cgctgttttt gattatttta ctttccagcg tcgtacagcc taacttctgg
    26161 tggctgctgg caattactgt tgtttggctg gatgagtctg gtcggcgtgg tgcgggcgga
    26221 gtttttacgt actcgtaatt tcgactacat tcgtgcggca caggcgcttg gcgtcagcga
    26281 tcgcagtatc atcctgcgtc atatgttgcc taatgccatg gtcgcgaccc tcaccttttt
    26341 accgtttatt ttatgtagtt cgataaccac cctgacctcg ctcgatttcc tcggcttcgg
    26401 tctgccgctc ggttcaccgt cactcggtga actgctgtta caagggaaaa ataaccttca
    26461 ggctccgtgg cttgggatca ccgccttctt gtcggtggcg atattattgt ctttgctgat
    26521 ctttattggt gaagccgtcc gcgacgcatt tgatcctaat aaggcggtgt agcatgacgc
    26581 aaactctgtt agcgattgaa gatttgtcgg tgggttttcg ccatcagcaa accgtacgta
    26641 cagtagtcaa tgatgtttca ctacagattg aggctggcga aacgctggcg ctggtgggtg
    26701 agtcaggttc aggcaaaagc gttaccgcgc tgtcaatttt acgcctgctc ccttccccgc
    26761 cggttgaata tctctccggc gatattcgtt ttcgtggcga atcgctgctt catgccagcg
    26821 atcaaacgtt gcgcggtgta cgcggtaata agatcgccat gatttttcaa gagccgatgg
    26881 tgtcgttaaa tccattgcat accctggaaa aacagcttta tgaagtgctt tcactccacc
    26941 gcgggatgcg tcgggaagcg gctcgaggcg aaattcttaa ctgccttgat cgcgttggta
    27001 tccgccaggc ggcaaaacgg ctgacagatt atccgcatca gctctccggc ggcgaacgcc
    27061 agcgggtgat gattgcgatg gcgctgttaa cgcgaccgga attattaatt gccgatgaac
    27121 cgaccaccgc actggacgtc tctgtccagg cgcagatttt acagctgttg cgcgaactgc
    27181 aaggcgagct gaatatgggc atgctgttta ttactcataa cctcagcatt gtcagaaaac
    27241 tggcccaccg cgtggcggta atgcaaaacg gtcgctgtgt cgagcaaaat aacgccgcta
    27301 cgctatttgc ctcacccact catccttaca cacaaaagct actcaacagt gaaccgtcag
    27361 gcgacccggt gccgttgcca gaacctgcct caacgttgct ggatgttgaa cagcttcagg
    27421 ttgccttccc cattcgcaaa gggattttga agcgcattgt ggatcataat gtggtggtga
    27481 aaaacatcag ttttacgcta cgagcgggtg aaacactggg tttagtgggc gagtccggtt
    27541 ccgggaaaag tacgacggga ctggcgctgc tgcgactgat taattctcag ggcagcatca
    27601 tctttgacgg tcagccactg caaaatttaa atcgccgcca gctgttacct attcgtcatc
    27661 gcattcaggt ggtatttcag gatccaaact cctcgctcaa cccacgactc aacgttttgc
    27721 agattattga ggaaggctta cgggttcacc agccgacgct ttctgccgca caacgcgaac
    27781 aacaagtgat agccgtgatg catgaagtgg gattagatcc tgaaacacgc caccgttatc
    27841 cggcggagtt ctctggtggt cagcgacaac gtattgcgat tgccagggca ttaattctta
    27901 agccctcgct gatcatactt gatgaaccga catcatcact cgacaaaacg gtacaggcgc
    27961 aaatattgac gctattgaaa tcattgcaac aaaagcatca actggcctat ttgtttatca
    28021 gccacgattt gcacgttgtc cgcgcgttat gtcatcaggt tatcgtactg cgacaagggg
    28081 aagtagtgga acaaggaccg tgcgcgcgcg tgtttgccac accgcagcag gagtatacgc
    28141 gtcagctact ggcgttgagc tgacgcttaa aaaggattgt agtttgaaaa gggttcggca
    28201 attgccacac caaaattttt caatcggcat gttgcagcaa actcgtcctg gcgatttaca
    28261 aacaggcacg gctcaccttc acactccagc accgaacatg gaacttcgat gtcgcttaac
    28321 gcctggctta gtttatacat caccgaccat gcattatcgc catccgggct aagcaattta
    28381 agcgccacca gtctgttgtc accgcacggt ccattttgcg gaatcggttc aaccttcgaa
    28441 cctgcgaaac cagcagacca gcgatagttc tgcggcagtc gatggacgat tgagagttgt
    28501 aatgaagtca cttattttcc ccggaagtac attcacttca caatttgttt acatcaattt
    28561 taacacatca tcaacaatcc gtctttatac agatcgtaaa gattgatgcc tcgcctgcgt
    28621 ggcctctatg gctctgattt aagtaatatc tgcgggcgtg gtcacgtttg cggggctata
    28681 tgtacccgtt tctgcgatct aactcaacct ttttaactac aatgatgtga ctttttacat
    28741 aaattgattt tacataaaat aaacatatat cggggaaatg atagatttgt ggttgacatg
    28801 catcaacaaa ggaagccttt taccttcctc gttgtgcaat agatcaccgt tttttcggcc
    28861 gactggcgta cagacagaag agaatggagc tggttgcgca gaatgcaatt gaccaaatca
    28921 tcggccatgc agagttaaag gtcgcaagag aaagcaatgc gccaacaatt gcccctatgc
    28981 caaaacggaa ggttcctgcc agcgaagatg ccgttcccgc catatgggga aactcatcaa
    29041 gaatgaccgc catcgcattg gatgacacca tcgacacgca gcccacaaac gccgcaacgc
    29101 caaccaccag cgaccagaat cccagcccca gcagcgcact gatgaccatc cacgctgcca
    29161 taataaattg tatccacaac cccgagcgga acatatttaa cgcgccaatg cggcgaacga
    29221 agcggctgtt aaagatggtc atcacgaaca ggaaaacaat gtttagtgca aagtaataac
    29281 caaagttttc cggcgcgacg tggttaattt caatgtagac aaacggtccg gcgctcagga
    29341 acgagaacat tccggcaaag ctgaaaccgc tggccagcat ataactcagg acacgcttat
    29401 gacggaacag cgccgcaaag ttaccaatag tggtacgaat gtgaaatggc tgacgacgct
    29461 ccggtggtaa ggtttcttta atcaggaaga aaatcattgc cgaagccaga atcgccgcta
    29521 atgccaggat ccagaagatg taatgccagc tcagccacac cagcacccaa ccgccgacaa
    29581 tcggtgccat caacggtgca atagtggtca ccagcatgac aaacgacatc atccgcgaga
    29641 actcttcttt cgggtaaata tcgcgcatca aagcgttgat aaccacgctg gccgcagccg
    29701 ccgccagccc gtggaagaaa cgcatcacaa tcagctgatc gatggtgttt gccaacgcac
    29761 acgccaccgc ggcggcggca aacaccagcg taccgccgag caccaccggc ttacgcccga
    29821 agctgtctgc catcggcccg tagattaact gccccaacgc aaagcccaga atataagtac
    29881 tgagggtcat ctgcgtactg cccgcaggta cgccaaactg cgctgaaatt accggtagcg
    29941 cgggcagata catatcaatc gacagcggca tcaacatggc cagcaggcca aggataaaaa
    30001 caatagcaaa cgacgaatgc tgtcgggtgg tcacaacggg ctcctgaaag tcatggaaaa
    30061 gttagacgac gctggcaatt tcttcttcag ttaacggacg atattcaccg ggggctaaat
    30121 cagcatccag cgtaataccg ccaatacatt cacgatgcag ctcaaccacg tggttaccca
    30181 cggcggcgaa catgcgtttc acctgatgat aacgcccttc gctgatggtc agacgaacct
    30241 gcgttggggt aatcacttcc agcaccgcag gcttagtgag atctttttcg ttatgcagct
    30301 gcacgccttt agcaaattgc tctgccgtat cgtcagctac aggtgattcc agtgtcacca
    30361 gataggtctt ctcgcaatga tggcgcggag aagtaatgcg gtgcgaccac tgaccatcat
    30421 cagtcatcag caccagaccg gtggtatcga tatccagacg tcctgcggcg tgcagtttcc
    30481 aggctaccgg ttcatccaga aaatagagca ccgttgggtg atcagggtcg tccgtggagc
    30541 aaacatagcc ctgaggctta ttgagcatga agtaacgtgg accatgttgc tgcgccagcg
    30601 ggttgccatc gtaagcgaca tcatgttcag gaagcagttt gaacgctgca ttacgaacaa
    30661 tttcgccatc gacggtgaca cgattgccgc ggatttcacg cccggcaata gcacggctaa
    30721 cgccgagttg ctgtgcgata aatttatcaa gtcgcatgtg tgtgattttg cctgtaaaaa
    30781 cggaggtcgg gcattgaccc gaaaatctga actgttgtct gcccagtata gcggtctaat
    30841 aacatccctc aagggaaaaa gatccatggc atactattag cagaataatc tacctacgcg
    30901 agaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc acgctcaacc
    30961 attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca ggtaaaagcc
    31021 tggtgattgc cgaactggca cggctggcgc gtggtcgtgt tctggtgctg gcacacgtca
    31081 aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa gccgatattt
    31141 ttgccgctgg gttaaagcgc aaagagagcc acggtaaagt ggtatttggc agcgtgcagt
    31201 cggtcgctcg taatcttgat gcctttcagg gtgaattttc gctgttgatt gtcgatgaat
    31261 gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact cacctgacta
    31321 aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga ttgggcaaag
    31381 gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa gcccttttcc
    31441 gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat ctgacgccgc
    31501 cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag gcacagagta
    31561 acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa cgcattacgc
    31621 cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta atgattttcg
    31681 ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa gatgctgcac
    31741 tgattactgg cgacaccccc ggcgctgagc gcgatgtgtt aattgaagat tttaaagccc
    31801 agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac gccccgcacg
    31861 tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa caaattgtcg
    31921 ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat tatgcgggta
    31981 atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt gacaacgttc
    32041 cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg aaaacgaccg
    32101 ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt gaagatgacg
    32161 acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg caatgtaacg
    32221 cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg gttgatccgg
    32281 atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc tgtagcggca
    32341 tgtctttgca gcatgagcac gacgagaaag gcgaatggtt gaaaatcacc tattacgatg
    32401 aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag cgaactgcct
    32461 tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg cgctggatca
    32521 ccgccgccga tatcctcgcc cagcaagcct tattgcgaca cccggatttt gtcgttgccc
    32581 gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa ggtcgttttc
    32641 gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataaa gcgattgagt
    32701 atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg ctgggtcgcc
    32761 tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga agtacgtaaa
    32821 gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc ggcaatcatc
    32881 tacggtggca aagaagcgcc gctggctatc gagctggatc acgacaaagt catgaacatg
    32941 caagctaaag ctgaattcta cagcgaagtt ctgaccatcg ttgttgacgg taaagaaatc
    33001 aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca gcacatcgac
    33061 ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg gggttttttg
    33121 tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga taaggcgttc
    33181 acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg tccggcgctg
    33241 caattggtca cgcaaattcg gcggcgtgcc tttaatggtc agggtatcgg ttgccggatc
    33301 ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc caccgccgct
    33361 accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc tctcttccag
    33421 ttcgtagcct ttttcagccg caaactccgt gaaactcact tcgctgacgc ctgccagctc
    33481 tttcgacagc gactccagtt cgatctcttc gcctgcctgc agctgctcat tacagtagct
    33541 ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt cggcagtgaa
    33601 atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgtttaacc cttcgctggc
    33661 cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt tcaggaaagt
    33721 gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac gcgcaacaat
    33781 atccgcatga ttgatatcaa gataatgggt tggattgatg tcgagatttt cgttaacgcg
    33841 catactgctc aggttgctca gaactgccac cagcagatac tccaccgcca gataacggta
    33901 gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt cgtcacgcag
    33961 gcgcccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac gctgtaagcg
    34021 cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat ttttggcgct
    34081 atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt ccagcaatga
    34141 atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt gcagagcaat
    34201 ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta ttcaaccacc
    34261 gccggttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc gtaacgagaa
    34321 tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg ttgccctttc
    34381 atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtgatg aacaggttga
    34441 acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga ccgacctttc
    34501 cctgatggtg ttaggcaaca tggtcactaa ccttatcaat accagcattg ccccggccca
    34561 acgccaggca attgccaact cttttgcccg cgccttacag tcctctatca acgaagacaa
    34621 agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc taccgtgaaa
    34681 aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt ctgctttcgc
    34741 tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt gctggtcgca
    34801 tttattccta cgtaagcatt atcggccatt tcagcttcct ggtgttcgcc acctacttgc
    34861 tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg tttttgtccg
    34921 tcattctggc aacggcggga atgacgctat tactgatcga tagcgaagtc tttactcgtt
    34981 tccatctcca tcttaatccc atcgtctggc aactggttat caacccagac gaaaatgaga
    35041 tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg cttgaactgg
    35101 tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcgg cgcttcgcgc
    35161 gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg tatatctggg
    35221 ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg ctttcgtacc
    35281 cgatgacggc gcgacgtttt cttgagaagc atggtctgct tgatgcgcag gagtatcaac
    35341 gccgtcttat tgagcaaggt aatccagacg ccgtttccgt tcagtatccg ttaagcgaac
    35401 tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc gatggcctga
    35461 actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag caaaatattt
    35521 cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt ggcctgttct
    35581 atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacccct gcggcgttaa
    35641 ttactgcgct taatcagcaa ggctatcagc tgggattatt ctcatcagat ggctttacca
    35701 gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta cgcacccaat
    35761 ccgacgagca gaccgccacg cagtggatca actggctggg ccgctacgca caagaagata
    35821 accgctggtt ctcgtgggtc tctttcaatg gcactaacat tgacgacagc aatcagcagg
    35881 catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc aaccgcgtgc
    35941 tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc actgccggtc
    36001 ggggtattcc gctgagcgaa gacgaagaaa cctttgactg gtcccacggt catctgcagg
    36061 tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct ctgactgatc
    36121 ataccgatct gatgacgacg ctgatgcaac gcctgctaca tgtcagcaca cctgccagcg
    36181 aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg gttactgcag
    36241 cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg aacaataacg
    36301 gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa ccacagttaa
    36361 gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga ttaattataa
    36421 atcagttagc aaaatatctt acttgcaatc ggtgtggaaa acggtagtat tagcagccac
    36481 gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg gtcggaggtt
    36541 caaatcctct cgtgccgacc aaatcgccca ccggatatca ccggaaagca ccggataaga
    36601 cagggataac ccgctaatca cttgattggc ggtttttttt atgtttttta gtcaccgaac
    36661 atcaccggat ttataagcta tcagccggat tttttagtta tacgtttagt tatacgggct
    36721 ggcgtataac taaagccgta taactaaaac cctgtttttt cgtgtggaat catagacata
    36781 tggcgagaaa aacacaccca ttaaccacag tgcagatcaa agcagccaga ccagcgcaaa
    36841 aggagtacac cctgcaggac ggcggagggc tttttctcct ggtcaaaccg tctggatcaa
    36901 aactctggag attttcctac taccgaccat cggacaaaaa aagaatattg ctgagttttg
    36961 gatcgcttga agatgtttcc ctggctgatg ccagaaaacg ccgtagcgag tacaggacgt
    37021 taatcagtgc cggaactgac ccgcaggacc acgagaggca aaaaagagag acagaggccc
    37081 gaagacaagg gaacacgttc gaaaatgtgg cggcggcatg gtaccaggtg aaaatcagcc
    37141 agaatctggc ccccaacacg attaaagaca tctggcgttc gctggataaa tatgtattcc
    37201 cgttcatcgg caacacgcca atagataccc tcaccgcccg aaggttcgtt gaagtgctta
    37261 cccccatcaa ggagcgcggc aacctggaaa cactcaagcg ggttttacag cgcgttaatg
    37321 aggtaatgga ttacgccgcc aacagtgggc tgattgatgc caatccggct atgaatgtgc
    37381 gtaaggcgtt tccctcacct gtaaaaaaac atatgccaac aatccgcccc gaacagctgc
    37441 cggagcttat gcaggcttta tcagtatcgg caacagaacg gcagaccaga ttactgattg
    37501 aatggcagtt actgaccgta acccgtcccg ccgaagcgtc atcaacgcgg tgggatgaaa
    37561 tcaacctgga cgcgaagcaa tggacgatac ctgccggacg catgaagatg cgcagggatc
    37621 acgttatccc gctttccggt caggctatgg cggtgctgga ggccatgaaa ccaatcagcc
    37681 accaccgcaa ttacgttttc ccaagtctga aagacccaca gcagccgatg aacagccaga
    37741 cagctaacgc agcattgcgg cgtatgggat tcgctggcgt gctggtgtct catggattac
    37801 gcgccatatt cagcacagca gcgaacgagg aaggattcga gccggacgta atagaggcgg
    37861 cacttgccca cgtcgacacc aacgaagtta gacgggcata caaccggagc aactacatag
    37921 aaaaacgcat cgtgctgatg cgctggtggg gcgaatttgt cgaggctgcg gcgacgggcg
    37981 taaccctcgc cagtggtaaa aggggtatcc gagccgtgta gctgtacaga aaaccagtaa
    38041 aaactacgaa aaccatgtaa aaccgtcgta taattgcatc aaatttaacg acaaggccgt
    38101 gaaacatgaa accgttaaga tgcaaaaaaa tatcagatgc aattgcgacg ggctgcaact
    38161 ggccctgatg gttcagcatg aattttggtc aacctacgat ccggaggaca gaacgacggc
    38221 cccatcaaaa aaacaggtag tagattttct ggtatcccgt ggcgcttcca gaaatctggc
    38281 ggtaagtatt gataaggtcg tacgtccggc atctatgaag atcggaggca ggcccaaaaa
    38341 atggcggtaa caatcctgga agcggcagaa atgccgcttt ttttataatt ccatttcaaa
    38401 tcatcaatat aaaaaacggt gtataccgtt taaaaacggt gggaactgtt tttacccata
    38461 tccgatgatt taccgtattt gtcaccggaa tacaccggat tcacaaggta aatcacgatg
    38521 gaagcaatca gaaaaattat ctttcgccag gaagtaaaaa aaattatcca catcaaggca
    38581 gacagcacgc tgcaaagcat gatcaacgcc ggagaatttc cgcagggttt tcgcgttggt
    38641 ttacgccgtc gcggatggta tgaggatgat gtgttgtcct ggcagaaaga acgcgaagag
    38701 gaagcacgcg gaacggctgc ttaacgggta tcacagatat gacaaacacg aaaaaagcgg
    38761 ccccgatatg gagccgcctt tctgaacaat taacccgctg cgccttatgt gtatgtgatc
    38821 ccaaacataa gcacggggat gatagccgct atcaggctgg tgggcaatgc aatcagtctg
    38881 gttcagttcg ttgccatacc tgcaatgagc gcttttccct gtactcttta aggaattgct
    38941 caagggcaaa agcacatggt gcgaatcttt ctgattcatg ctctatcttt ctgcgccgtc
    39001 tttttcgtgc aggtgatgtt ttttcggttg attcttcgtt ggtcattgtg tgcgcctgta
    39061 aagcaatgcg ccggagtacc tcacaccacg gcgctgatag tttttatcct ttgggttcta
    39121 tgccgcgttg ctgtaattct ttgcggatta ttcgctttat ccaggcagca agagaatcat
    39181 cgccgtcctg ttgttgtgcc ttttccatct tttcccgtaa gtcaggatct aaacggaact
    39241 ggaacggagg attaccacgc ctttcgtttt tgtgtgttga cacgttaatt acacctcatg
    39301 taatgtattt atgtgtaatg acacattaca cacaatcaac acaaaaagca aagccccgca
    39361 ctgtaggagc agtaacgggg cttctaacca acaacgtaaa ctaggagccg ttatggttgc
    39421 tgtaaatcat ataccacacc ttgtacacac acaaacggcc tttgtgtggc gttttctggc
    39481 cctgaatatc ggagaaaaaa accgcttgat cgccaccagc aaaaatggct atgattcccg
    39541 cgcacctcat aaaacgggtg tcgggattag cctcctgaaa acaactgaag cgcacagcac
    39601 gcgccccgcg tgtttttttg tgccgcatag tcacacctta tcaatggtgg gctgtacggg
    39661 ggcggagcaa tccgcgccgg tttcttcagt gtccggtaag gctaaccctg tacagtccgc
    39721 caccagcgaa attagccttt tgcgtggcgg ttatcttaac cacactgagg aggctgccaa
    39781 catggctact atccctaccc ttgtacattc tcaaaccgca tttatctggc gctttatcat
    39841 ctttggcgcg tcagaatctc aaatcatcca cgtaaccgcc tggacggaac gcgaagcgcg
    39901 tagccgttgc ccgtcaggtt gtgttgctgt attcgccgcc cgtattcgtc aggggtcaca
    39961 tcatgcgtaa aaaccgcttg caaaaaatta taacggggct gtatgcttcc ccagtcgccc
    40021 acatggcgac cgggtttagc agcctgaata ctacagcgga cagccgctta cattccgata
    40081 tgcggttttt ttgtgtccgt aaacctaccc atacccgcat tatggcgggg cgtaacgggg
    40141 gagcctttgc gctcgctggt tcctgtagtg ccagtctgct aaccctgtta cgtctcgcca
    40201 ccccgtttag cagcgtagta gcgagactcc ttaatttcac tacaggagcc tttcacatgg
    40261 ctgtattcac acgcccttat tttgtctggc gctttatgca gtgcctgaca gacagcattg
    40321 caatattcac cgttaccgca gccactgaac gcgaagcccg cgcccagttg ccgcacgcac
    40381 atctgatttt tgtcgcccgt attcgtcagg gggtgtgcca tgcctgacat gaccaattac
    40441 cagtacctga ttaatccgca ttttaactgt gagcatgata ttgctaaaaa ggtttattcc
    40501 gctgcggatg gggctactga caatatatca atggctgttt cgtcaattgg tagcctgatg
    40561 tggtatgcgt cagaaaataa ggaatatgac gaaaaggcca tgcgcattga tatgggtaat
    40621 atcggtttgt tactggcaat gcttgggcag tttgatattt cgttacggtg caccattgaa
    40681 aatgccaaag atgcattaaa tgctataaag aaagcgaata ctgattcaaa tcggggataa
    40741 ataatcatga gaacatattt atctggcttg actgccagcg gccccgctcg gcctgaaatc
    40801 cgccccggag atattttcag agataactac ggtggcgttg ttgagattaa aggcgtggca
    40861 gaacggcgca ttacctaccg ccgtgaaggc tacgaatatg attgcgtgat gcctgtttat
    40921 cagttccggc gtgatttttc tctggtacag gcagcgcccc gcagtaaacc aaccagcagg
    40981 gagaaagcac gcgccaatat tcaggaaata agaaagatgt tgaatgtatt caggggtaaa
    41041 aaatgaaact ggcaccgaac gtaaaaaaac aaccacgcgg aataaaacac aaagacacag
    41101 aggtaattat tttcgcgggt agtgatgcgt gggcacatgc aaaacagtgg caggagcagg
    41161 acggccccgc atccggcgat aatgtgccgc ctgtgtggct tgggccaaat cagcttgccg
    41221 aacttgatgc actgaaaatt gttcctgatg gaaaaaaacg cgtaaggctg taccaggccg
    41281 gagaactgga tttggtggag accaaaaaga ttggtcagaa gctggcggcg gcagatattc
    41341 aggacgcaaa tttttacccc gaaggaatgc acgtccagaa gtgtgaaaac tggcggcgct
    41401 atctgaatgc tgagcgtgaa aatattgccg cagggcttac catgccggag cagaaaaata
    41461 cgcaactggc acaaatggca gacagtgagc gcgcgcagct gcttgccgag cgctttgatg
    41521 gcgtttgtgt gcatccggaa agtgaaatcg ttcacgtatg gcgcggcggg gtatggtgtc
    41581 cggtcagcac aatggaactt agccgcgaaa tggtggcgat ctattcagag cacagggcca
    41641 ctttcagcaa gcgcgtaatc aataacgccg tggaagcgtt aaaagttatt gccgaaccaa
    41701 tgggcgagcc gtccggcgat ttgttgccgt tcgccaatgg tgcgcttgac ctgaaaacgg
    41761 gggaattttc cccgcacacg ccggagaact ggatcaccac gcacaacggc attgagtaca
    41821 cgccaccagc accaggggag aatatctgcg acaacgcgcc aaactttcat aaatggcttg
    41881 agcacgcagc cggaaaagac ccgcgcaaga tgatgcgtat atgtgccgcg ctgtacatga
    41941 ttatggcgaa ccggtacgac tggcagatgt ttattgaggc caccggagag ggcggtagcg
    42001 gtaaaagtac attcacgcac atagccagcc ttctggcagg gaaacagaac acggtaagcg
    42061 ctgaaatgac ctcacttgat gatgctggtg gacgtgcgca ggttgtcggg agtcgtctta
    42121 tcgtcctggc tgaccagccg aaatatacag gcgaaggaac gggcatcaag aaaatcacgg
    42181 gcggcgaccc cgtggaaata aacccgaaat atgagaagcg ttttacggcg gtaatcaggg
    42241 cggtggtgct ggctaccaac aataacccga tgatattcac cgagcgggcc ggaggtgtgt
    42301 cacgccgtcg ggtgattttc cggttcgaca acattgtaag ggaggacgaa aaagacaagg
    42361 atttgccgga gaaggtcgcg gctgaaatcc ccgtaattat ccgtcgcttg ctggctaatt
    42421 ttgccgaccc tgaaaaggca cgggctttat tactggaaca gcgcgacggt gatgaagctc
    42481 tggcaataaa gcaacagacg gatccggtta ttgagttttg ccagttcctg aattttctgg
    42541 aggaagcgcg cggcctgatg atgggtggcg gtggcgattc agtgaagtac acgaccagaa
    42601 acagccttta ccgcgtctat ctggcgttta tggcatacgc aggcaggagc aaaccgctaa
    42661 acgtggctga gttcagcaag gccatgaagc cagcggcaaa agtttacgga catgaatata
    42721 ttacgcggag agttaaggga gtaacgcaga ccaacgcaat tacaactgat gattgtgacg
    42781 cgtttttata attttttgta aaagccctct accccatcta cctgaatgaa ataaacgcat
    42841 attattcaac atgataagtg ggtagagggc taggtagaag gctaataaaa cctctctacc
    42901 tcttctacca tgattaagat cgtttgtgag gggcgggtag aggatgggta gagggccacg
    42961 aaagccctct acccatctga aagccgcgcc attactgaca tgaaagatga ttaggtagag
    43021 aggtagagga ggtgcaccac aacctaaaac tttttaaaca cgagggtaaa aataaaaatg
    43081 cacacatcag gaagatttaa caaatcactc aaaaaacgca gagacagaac agaaccgaaa
    43141 tatcgcgcgt tagacatgac agagcacgct ttaaaggtgg caatcagaac gatagaccgc
    43201 cacgcggggg gaaggatacg cgaaagcaca tcccgaactg ataagcgcat tcatgaccac
    43261 ggcggcggca aactttgcca cgctgacaga acgggagatt gccgaagcgg aacaggtaac
    43321 aaccatcaac gtaaaaaccg gatagtcagg cgatgataca tgaccgcata gcggaggaac
    43381 tcgaggcaaa aggcttttac cggagggcat cggcgcgatg gggtgaagtc atgctgctgg
    43441 tggagacaga caaggaacgg catcaggtta cgatgcgacg gctggaatgt tccaggaagg
    43501 cacagaagcc accggagccg ccggataact tcggagacct gagaaaggca gtagatcgca
    43561 cttatgctga aatgggtata gatggtgtaa gcgatgaaat atggcgtaat tacccagaca
    43621 gctaatcaac agccggagta atccggcttt tttgtaccca aaaaaagccc gataagtaca
    43681 ggaggcatct tatcgggctt ttgcatatga ggtttttttt ggtgcactga cacacatgat
    43741 cgggatcatc atttcataat ttgcaacaca actcaacatc attgcataaa atgcaatcat
    43801 gattataatc agatctggat gaacatccag ttatgatttt ttaagtcaaa gaggaatttc
    43861 ttactatggc tgaagagaaa aaaggcggtg tttcggtgta cataagcccc gacatcgtga
    43921 aggcgctcaa ggaacgccac cagcagaacg taaaagcagg cattgcggca ggacttgatc
    43981 cgctggcgat ggttgagccg tcaacaggct ggcaggtacg cgcctattta cgcgcggcgc
    44041 tgggtatgaa tcaggttcac gggggtgaat aatgacagta aaagcaatag cacttaacac
    44101 taaccagctt tttgcgtacc tgaatcgcga ggatattgcg gaatttaaat tcagtccgct
    44161 gtttaccgcg ctgtttttcc cgaacgtggc gacattcaac acacaggaca tcatgttaga
    44221 taacctggat attgaagaag tcactatgtc ggcgttttgt tcgcctatgg tgggtagcca
    44281 ggttcagcgc gataaagggt acgaaacaag cattattcgc cctggctaca tgaagccaaa
    44341 acacgaaatc gatccattaa aaacaataat gcgcatggct ggagaagatc cagcacagct
    44401 taacgaccct acctaccgcc gtatgcgcct gattactggc aacatgcgcc gccagataaa
    44461 cgccattaaa gcgcgcgtgg aatggctggc ggtaaatgcg gtaacgaccg gaaaaaacat
    44521 cattgagggc gaaggcatag agcgctatga aatcgactgg aagataccgg aaaaaaacat
    44581 catagagcag gccgacggta aaaaatggtc cgagcaggat aaagacatac acgatccaat
    44641 ctatgacatt gagctatacg cagatcaggc aggttgcccc gccaacgtca tgattatggg
    44701 cgttgatgta tggcgcatgt tacgcagctt taaaaaattc cgtgagctgt acgatctttc
    44761 ccgtggttca gaatccgccg cagagctggc atgtaaaaac ctgggcgaag tggtgagctt
    44821 taagggctat cttggtgatc tggcccttat cgtctattcc ggcaaataca ctgacagcga
    44881 cggtaccgaa aaatatttcc ttgagcctga tttgctggtc ctgggcaaca ccaacaataa
    44941 agggctggtg gcctatggtg cgattatgga acaggaagcg gtcagaactg gcgcaacgca
    45001 aaacatgtac tacccgaaaa actggattga ggacggcgat ccggcgattg agtacgtgca
    45061 gacacacagc gcaccgcagc cggtaccggc agatattcgc aaatttgtta ccgtcaaaat
    45121 tggttaacgg gggattctat gaacactcca tacgttgagt tatttgcagg cagtcagcag
    45181 gtatcaacga cgctggtaca ttttgccgct gatgctggcg ttattcagga atttaccccg
    45241 ctgatgctgg cggacaatgg cgagtttaag ccgtgggatg gtcaggaatc tggcaaggct
    45301 gtttatctga cctcgtaccc cgttgacacg tcgacgcaga aatcagcaca gtgctataag
    45361 acggggatat ttaatatcgc cgccgttaac tggcctgaga gcgtcgacac cgatgcgaaa
    45421 aaatgcgccg cctttgcggg ttccggcgta tccgttcagc cgctggcccg ataagcaggg
    45481 gtaacgatgg caacgaatga aagcatcatg acgctaccgc tggcgagtaa atttaaagcc
    45541 gaagcgagtg caatggctga cagaggttta tcaacctatg aggccgtata tcaactaaac
    45601 aaactggaag agcaggacaa gccgcgcgct gatgcgatta tggcgcttca tgagcataaa
    45661 gactatcagc cgctgttacg tgcaatggca aacgtgccat gtattagcgt cgataatgct
    45721 cgtgaaatcc tgaacatgac catagagcag gagcgcccaa aggttgcacc agagcttacc
    45781 gcagcctttg aaaactttat ggacatgcac agcccgcaag ccgtatcagc tggcatggca
    45841 tacgatggca gaaaccaggg cgatgacggc gacatcgatc gcatactgaa aaccatctga
    45901 gacaaggccg gagaaatccg gcttttttta cgggtccttt ccggcatatg gacccgttac
    45961 ggggcggcga cctcgcggtt tttcgctatt tatgagcctt ttcagggggg tggtggtggt
    46021 tttgttgttt gatctatctt tatgaatgaa aagggaaaga tgcaagcaat acaccaacct
    46081 gaagcagtaa ttaagttggt gtattaatga aatctcatct gatgaacaaa aaaaacatgg
    46141 cgaaaagctg ccgtgtaagt gcgacagcgt tcgacaagtg gggagtaact cccgttgagc
    46201 gtaaaggccg tgaggcgttt tatgatgttg ccagcgtgat agaaaatagg gttaacaatg
    46261 caattagcca gcttataaac gacaaaggcg agattgacga tgatgaactt ttacgagtca
    46321 ggatcagatt actgacagca caggcagagg cgcaggaact taaaaacgat cgcgatcgcg
    46381 gtgacgtgat tgatactgag ttttgcctgt acgcgctttc aaagctggcg agtcagattt
    46441 catcaatcat ggacagcctc ccgcttacta tgcaaaggag cttcccacag attacccccg
    46501 ccatgctgga taggcttaaa agggaagtgg ttaaagcctg taatgccagt gccagagttg
    46561 ccgacaacct cccacagata ctggctgatt acttgaaaga aacaaccgga aacgtaccgg
    46621 aaaagttgct acagaagaaa ggcgagtaac agacgcgcaa ttattgaaca aaactgagaa
    46681 acgacatgaa agcgtcataa atcgccattt tagatgatta ccgtgtcgtt tctttttatt
    46741 gtgtatctat ttaaaaacaa atagttatgt tcgagaagta ccgacatgct ttttccagaa
    46801 aaattttcat aaacagataa aaaccgcgag gtcgccgccc cgtaacgggc cataattcca
    46861 ggaaggaccc gacgacacca gactatcaga acgatggggg cacaatgaca gaagccgaaa
    46921 tactgggatt aatccgccgc gttgccggaa tcagccagca ggttgacgaa caggccacgc
    46981 agccggacag cgtgaccgct gaaaattatg cgcgtgttgt tgcggaggtg atgcgccgtg
    47041 atggtataga acttaatggc gtggatatgc gcaacatacg aaccagagtt cttgagttgc
    47101 tggcctaccg tcgccgtgtg gagatgtatc gggagaagga gaaaataacg taccactgga
    47161 agaagccgga gcgtttgcgg cggtaactgg ttgatattcc cgacggacca aaaatgggtg
    47221 gctacatccc tcgcttctga ggactgttaa caagctgcct gaaagacagt cacaaactac
    47281 gaaattttcg cagtttaatt aatgatcagc ttaatcgcta acccgctgat attttcggaa
    47341 acctcaattt gaggaagtcg gcgcggtaac tcgctgaact ttaagcaaag cgcaaaattg
    47401 cgcttgctga ataatcatta tgattacgca gatgattaag gaatgaccga aggcggaaat
    47461 tcgcctgtgg ttaatggggg agttgcagat ctgcaactcg accatgaaac tacggaaact
    47521 acccgtagtt tgggtagtaa gagtaacacc cagattttgg ggcttactcg tgatacccaa
    47581 atttggggta tcggtggcaa ccataacgac tttcgttacg gttgatgctt ttaccccatt
    47641 ggtggaggct gggcggcctg ctgtgggctt ttccaccatt ggtggaaagg tgcagatctg
    47701 gatcatcatt acggtgatca agggtatgta aaacccacca gcctgattaa cagttaaccg
    47761 gaaaaaaaat ccggcatggg gggggagtgg ttagccacga acaacgaata agctggacac
    47821 cgcgccccct ctcacgcaga gaaaaaatta ccgtttcgta gtagttaaca tgttaactgc
    47881 ctgatgaagc gttgttgttg attttttctt tatgattcac ctggttgcgt gcaaaaaatg
    47941 ttaactacct ttttggtaaa aatgttaaca gggcagcggt taacattaac caggcgtaaa
    48001 tcccgatatg gtgatcccca tatcggggaa catccaccag cttaaaaacg aatgagcgaa
    48061 aaaaatccag gtagaattat ggaagtcgct agggcaatcg ccgtttaaat tgtgcatttt
    48121 ttcagcaaat tattttagtt atacgtttgg ttatacggct taaaagttga atcgataata
    48181 ttgattaaaa tcatactgtt acacgctcaa ttaaactact ctcgtgccaa aaatcccaag
    48241 aaaaaatcaa cctttacggt tggttttttt tatatctgca attaattcga taaacagacc
    48301 gtgacacatc acagcctgtt tattttttgt tatcagaacg tccaggccac acccgcctga
    48361 gtattccacg gcgattctac ccctgcgcca ttgccatagc tgactgacaa atgaccgctt
    48421 aacgtcgggg taaacgatga ccttatccca gcctgataaa cgccacgggt tccagccaca
    48481 tcattattaa atttgccatc gtcattaact ttcacctggt tagaatcggc gtattcctga
    48541 cgcacggcgg ctttcagtca gggttccagc gtcgtaccgt tttgcaggtc catgtgatag
    48601 cttaccgccg ttcccgcttc agcgcgtaat atccgggtat ttcccacatc cgcgcgcatg
    48661 ccgtttgata acgtgtagtc ctgaccatct gtggtaaagc cggtaaaggc cagatagggt
    48721 ctaacactcc acaatccgtc aacccaacgg aacccgctct cgacatgagc acccgcgccg
    48781 ttactattgt aatcgccaaa cgctgttgcc ccattactca tcttgcaatg gatggtgttg
    48841 gcaaaacggt caactttcac cactccatca acataggcac cgttctgatg ctcccaaccg
    48901 gcataagccc ccagggtata gctatcgaca ttgcctttgc cgccgcgatc aaaaccaata
    48961 tcagaatgag agtaaccaaa gaacaagccg cgaattgtgc tggtttcttc acgggagaaa
    49021 cggctatcga taccgagcgt cagcccggtc aatgtttgct caaagccagc gcccgcatca
    49081 gtggtcacgt tgttgcgggt gttaattgcc gaactccaca tcgccgtatc gtaattaacg
    49141 ccttttacgc taccaagacg ctcacgcacg gtgtccagtt ctgcatcaaa taccagcggt
    49201 tgtgctgccg ccatattcag cacatcagtg gttgaagggg taatttgcgc gcgattctct
    49261 gccaaactcc agctatggtt gccattatcc agcaaggtat attcatacgt accgatatca
    49321 acaacgcctc cggcattgcc caacgtaaat gcagcatcac cgccgcccgt tgttaccagt
    49381 gtaaggctat ctcctgctgc cgggctggca ccggtgtccg tcacgaatat tttgaaatca
    49441 cctgttgcct gaccggtgac gttgagctga tcgctctgat gattagccat atcggtacgc
    49501 atataaaaat tgccgtttcc cgacagggta ttggtggtca acgtaatgaa attttccgca
    49561 cttgccgtca ctgatgaacg agtcactggc tcatccatca taatcacatt tgcgtcagac
    49621 atcgcaaggt tggctaagtt gaggagggtg ttttggttcg tcgtagtgat atcggcaatg
    49681 atggtccata cgctattttc gagcgaaacg tccgctttgc tggtatccac ggcaccatgc
    49741 gttctaaaag aagcgccctc agaaaccact accttaccca ccaacattgc gggtgcataa
    49801 tttatttgcg gcttcatgac ctcaagaatg ccatcattcc ctctgactga aacgttaacc
    49861 attgtgccag cccagacgtt agcgcggcca ttgttgataa ccatattttc agaaatggct
    49921 tttgaggaat acgtgatact tccattctga tctgatcgac cgagatcata taccgcattt
    49981 gcctgcactt tcgtaccagt atcctttccc agcgattgca tagtggcatg ctcatcaagg
    50041 atagtatcaa tggcctgcgt gtcctccata acaatgagcc cggaaccatc atccagttca
    50101 taattttttg acacaccatc ttttatacta aattggcctt tactgtttgt accactcact
    50161 tccagcgcat tcgttgagac aattaatttt ccgcctgctt ttttatcaac gccagttaca
    50221 gtcccgccat ccataacctc cagtaagccg ccactatcta cagtagtatt ataagcgaag
    50281 tcattttctt caactcgcaa acttccgccg ttttccaaca gcatattgtt agcaatacca
    50341 ttcttgattt cgaactgacc gagacggttt gttccgaatg cctccatgac ccgcgtgctt
    50401 gctttaatag caccgcctgc tttctgatct accgcaatcg ctaatccccc ctctttaacc
    50461 tccagtaatc ctcccttttc gatagcggca aggacggtaa tcccatcttt ttcaacgatt
    50521 aagcgcccgc ctttaatcgt gggatcattc gctgcgccat aaacctgtag cacaccatta
    50581 ttgagggtag tctggtttgc cgcgccaccc atttgaacaa tttgataacc gtcatttaat
    50641 actgagtatt ctgcggtacc attcatttca atgtactgat accctccttt aatttcagta
    50701 ttactactta caccaaattc ttttatatgc tgttctccgc caggattgat tttcgtatta
    50761 ttggcggtcc cataaaccag ctggatatct ttttccagga caacaccatc aactgtctca
    50821 ccatactcaa ctgttgatgc caatgctatg ggtgaatata acgctgtagc caccagaaca
    50881 gatattgcgg aacgacaaag agaaacagaa ccagattgat gaattgagct ttcatcctat
    50941 gaaattaatt gctgttaaaa gcattgggta cggaaaatac ccatagctcc atacccggag
    51001 tcagttttta aa
//
LOCUS       DAZGVQ010000013        50777 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000013 DAZGVQ010000000
VERSION     DAZGVQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50777
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.013"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..99
                     /locus_tag="ACHLAG_000733"
     CDS             <1..99
                     /locus_tag="ACHLAG_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFP0654016.1"
                     /translation="IILAACQANYIRDVQGGTVSPSSSAELTGLGG"
     gene            complement(346..660)
                     /locus_tag="ACHLAG_000734"
     CDS             complement(346..660)
                     /locus_tag="ACHLAG_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654017.1"
                     /translation="MITVIISEVDGWREWKHRARTMDAQTAIIRAMNKHFPRSYIFIP
                     DDIDNAPVLFAAVTRTPNVKITGHIWKPMWNRGICWNVKGPPVIITLIQGAAWNSENK
                     PR"
     gene            complement(728..1384)
                     /locus_tag="ACHLAG_000735"
     CDS             complement(728..1384)
                     /locus_tag="ACHLAG_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFP0654018.1"
                     /translation="MGSVTKKPSPVVIIKKKRRILSHQDDAVQAVPDDIKPAPVMEFK
                     TDSLPPEIPATVAGSPEQQKKTGRKKKKKKKNNRWNHEFIRHSLDCVKKTFPQLRAEE
                     GGYHAFKIGISKDVMAFISASPECGLTVKEWNCAVRMITGGYKYLQRISVAGTPRYGL
                     DGKVHGTVTEEEALYAQAKLEKHTQRYNARMKETEDARCNQPSTNCHTGQQFEGRLHS
                     "
     gene            complement(1487..2077)
                     /locus_tag="ACHLAG_000736"
                     /pseudo
     CDS             complement(1487..2077)
                     /locus_tag="ACHLAG_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536605.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
     gene            complement(2181..2468)
                     /locus_tag="ACHLAG_000737"
     CDS             complement(2181..2468)
                     /locus_tag="ACHLAG_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0654019.1"
                     /translation="MTYTVKFRDDALREWLKLDKSIQQQFAKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRERSNVYNLASERMR"
     gene            complement(2465..2716)
                     /locus_tag="ACHLAG_000738"
     CDS             complement(2465..2716)
                     /locus_tag="ACHLAG_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP0654020.1"
                     /translation="MPNIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYC
                     VPAELYERMLDAMDDQELVKLVTERSNQPLHDVDLDSYL"
     gene            complement(2789..2980)
                     /locus_tag="ACHLAG_000739"
     CDS             complement(2789..2980)
                     /locus_tag="ACHLAG_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654021.1"
                     /translation="MAENGYGLAGLGMGKVKSINQYRLTPGFGGFTLVPYVVTSCRLP
                     CRSRGIRTIKPALNVFAKV"
     gene            complement(3676..4548)
                     /gene="repA"
                     /locus_tag="ACHLAG_000740"
     CDS             complement(3676..4548)
                     /gene="repA"
                     /locus_tag="ACHLAG_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFP0654022.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREGAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLAELGLITYQTEYDPTIGCNIPTDITFTPALF
                     ASLDISEEAVASARRSRVEWENRLRKKQGLDALGMDELIAKAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
     gene            5384..5890
                     /locus_tag="ACHLAG_000741"
     CDS             5384..5890
                     /locus_tag="ACHLAG_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005082529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFP0654023.1"
                     /translation="MNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILK
                     KVRRTDKVCGYRRHIHAVFPGYFFLKADPERHSFTHLRRHSAFLDFVKMAGEIKTVRE
                     DIVQSLMKVYPDPALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHL
                     VSDDGALT"
     gene            6030..6692
                     /locus_tag="ACHLAG_000742"
     CDS             6030..6692
                     /locus_tag="ACHLAG_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFP0654024.1"
                     /translation="MTIQSPENPVIQLLENMLEALSTSPDSINNERRRRRFLLNWLDS
                     VRQMSEFRGMPEEFTALRKLLATNTDIPVENTLKSLLESGHNAALYDLYRFRAALATC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTGDTFSTTGRMIRPVAFSLIHP
                     ATINTSEVEEIFRDEGFMLAHGRECSLNGSGRNMLSRILHVGHSGLAEEEWGMDNSLL
                     LH"
     gene            6959..7579
                     /locus_tag="ACHLAG_000743"
     CDS             6959..7579
                     /locus_tag="ACHLAG_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFP0654025.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLTP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            7606..9300
                     /locus_tag="ACHLAG_000744"
     CDS             7606..9300
                     /locus_tag="ACHLAG_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP0654026.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTARVHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIDGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV"
     gene            9381..9623
                     /locus_tag="ACHLAG_000745"
     CDS             9381..9623
                     /locus_tag="ACHLAG_000745"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFP0654027.1"
                     /translation="MSAEKFRVVIYTNTCEKKVSYIPATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            9740..10075
                     /locus_tag="ACHLAG_000746"
     CDS             9740..10075
                     /locus_tag="ACHLAG_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654028.1"
                     /translation="MRTITTHEQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KAQQLVESCEKIAEGKLPVTCPVCFAIWQDVHRFTHVDFDTQSGKSDFIDTCHSEIIT
                     GNVSITGKK"
     gene            10232..11299
                     /locus_tag="ACHLAG_000747"
     CDS             10232..11299
                     /locus_tag="ACHLAG_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin co-regulated pilus biosynthesis Q family
                     protein"
                     /protein_id="HFP0654029.1"
                     /translation="MQKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPPPVTRPVTPPRPTTTATPTTAVSSQSTALPPRPLLQATQLPALVMT
                     GKPGQSSVVYLREARQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHR
                     ALNTLLTGQQLYGHMDWNRKILTVTTTATPPVDLTAPQGSQKTADTPRNPFRSTTGTA
                     ADQAQVPPAVSASRVQSTTGTSGTSAPRTTTPAPVVTKPVAPPQKVTPPRQWKMEKGT
                     TLKDGLARWTSSEKCSHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPVEK
                     PLYARTSTAQCLLRVTDRPQE"
     gene            11304..11741
                     /gene="pilM"
                     /locus_tag="ACHLAG_000748"
     CDS             11304..11741
                     /gene="pilM"
                     /locus_tag="ACHLAG_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFP0654030.1"
                     /translation="MGWFIVFIMVITTTGNFWFTSQLSRSEHRHQAAENTQQAATFIR
                     YMNAINDYLHQHPERRTAGGRLTSAQLGIPGTDTVSHIISQQRVFVWATETPGLMAAL
                     REQSNDSALLGRVENGRLLDTAGRALSVTLPSVIPDHVILWMN"
     gene            11772..13391
                     /locus_tag="ACHLAG_000749"
     CDS             11772..13391
                     /locus_tag="ACHLAG_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFP0654031.1"
                     /translation="MNKKILAIMIAAALLQGCASYRQLESTRKEAVAEQQRAREMMSD
                     LRTRTPVVKESSRQWINPRPVMDKNTGKTVPGCPIVIHTRQSITLQQVVQRITESCHI
                     PVRITPDVLTYLNTSTTGSTQQIQGALPPPDASGMVPLAAIGASDTPPSHLPGNTAAL
                     SELNASGLTTLLNAVSSRLGISWRYDGSHIIFYYLETQTFPITYMDSQVAYNATVVSG
                     TMSSNGSSGGSASDAISGDASNTQTTTVDMKSALYQDVKNAVTSMLTPGIGRMFMSTG
                     FITVTDNPQVLETVRAFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDAITGGVSIVDGKLTGSKAFLKALSSQGDVSVVTQNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            13412..14707
                     /gene="pilO2"
                     /locus_tag="ACHLAG_000750"
     CDS             13412..14707
                     /gene="pilO2"
                     /locus_tag="ACHLAG_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFP0654032.1"
                     /translation="MTDISPTGRQVFPLTAGRNVFLAGLEWKTLPSQYRHARDFARAQ
                     KADLFLACQYLSNEDADTHTMVATVSRRILPGKPRQCFSLALLILPLLEHGGYAITEL
                     TLPGETPHYSFVSVVDGVLVSDLVGSGEEVREARDTFLSINTEPTQGWIHYEPAAFSA
                     GAQNQHLPLASLTGSGKHPAAARLNPVSRGPQFITVSLIIALLGTAWYGWQYYERWQT
                     EKAAQAAVARQATEERITPPWPGLPETDAFIRRCADIWTSLPLSAAGWRYSLAECSTD
                     GGSGNLRASYTNTAGATVTDFIRRISDMTDSRPFIVMPEGKTGGIGLPVTFRLSENAV
                     TVDALPETAVLQERLTSLAQSMQLKLDWQEVNNSVTDENGQIIQPPWKEYDLQIQTTL
                     PAHHLAERLKEPSVRFISVTRQLENGRFHYQFTGKYYAR"
     gene            14697..15149
                     /gene="pilP"
                     /locus_tag="ACHLAG_000751"
     CDS             14697..15149
                     /gene="pilP"
                     /locus_tag="ACHLAG_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFP0654033.1"
                     /translation="MPDKAKTLLFSVLLLPATAMATTGNAEQQNTQQIRIEQIQEETA
                     LYEAMLAREKIRHELRTLEQGGPAEAGRQQESISAVTTNTQASLSPRLTAIFGVQPQL
                     TARIELPGGSISDVKAGQNIPGTTYHVEKITPDSVVLEQSGTRKTLRP"
     gene            15234..16742
                     /locus_tag="ACHLAG_000752"
     CDS             15234..16742
                     /locus_tag="ACHLAG_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFP0654034.1"
                     /translation="MLDPADLPPDDILDYVAIDTDNAGQLRIRVVEGKKHLRAVQEYL
                     TRLRARYQGRVGDFEFTTLDVIARIREATTIAGQENGMNPVQNKMLGYIRHAATLNAS
                     DLHITPGRDNTDFTYVEARVHGELEILDIIRKDEGVELLGATYSGMTDVIKGTQFDPG
                     VPQDARLAEKFLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSSHIPTFGMLGYHPKQE
                     QTIRRILQRPEGIITLSGPTGSGKSTTLRTASAAYLEQHGFNNTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDSAQGWVDSIKSALRLDPDGILNGEIRDHDSAITAIKAAMTG
                     HLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLICPHCRHPWNDV
                     ASRRTEDERRLVETFCNADAVYLRNHDGCPHCWRGVTGRTVIAEVISPDARFFQLYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGLVDPLAAHYISPIDEDSYTLLR"
     gene            16744..17844
                     /locus_tag="ACHLAG_000753"
     CDS             16744..17844
                     /locus_tag="ACHLAG_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFP0654035.1"
                     /translation="MKFLEKLNSTELDLSALNSWTARKTFGLKRAEFYESLGGMIRDG
                     TPSLRALEFICNVETDFGTRKGTSGMYFLARECIASLKASGMLSGALPTWVPPEEAAL
                     IRNGEERGNIAEALFQVAKTVKARREMVSSLITLCLYPLLLLSLCVVNMYNAHTRIIP
                     IVSAFAPEEKWSFQMKVLAAMSELVIGYGLWLSGAIITLAIIIRFSLSRYTGPGRRLL
                     DRLPPWSLYQTFHGVNYLFSISSMLQINIPLSQALLRIEKSAEHNKWLKERIQAIRKN
                     VLSGQNLATAMRNSDYDFPSKICINKLLLNSEREDSVSAMNTYAGQWLEDAKKSVRKS
                     GLIITGISGGFVFFFILNMISAIYSISDMLKQ"
     gene            17918..18454
                     /locus_tag="ACHLAG_000754"
     CDS             17918..18454
                     /locus_tag="ACHLAG_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFP0654036.1"
                     /translation="MNFRARLSSYFSLKKDKEEDKGLTLLEIAGAMIIIAIVVALAMS
                     QMSSTMASSDNQRELKNLQTIATKMKQYKFQGRYTGTDYIKTLTESGALPADMIAGGN
                     KAKNAWGGDVTIAATADKYGYIITSNNVPKTNCVELINSLRSSSIFTKIMNTAPNMVD
                     PVTVCSSDKNNIKLETNS"
     gene            18508..18981
                     /locus_tag="ACHLAG_000755"
     CDS             18508..18981
                     /locus_tag="ACHLAG_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFP0654037.1"
                     /translation="MKWFYLSSLFLFCLPATAAVNSNTLDSCFIQAGKRYSIDPGLLK
                     VIARKESSLNPRAINHNRNASGKIISTDYGLMQINSTHIPGLKRMGIIRSENELLSNV
                     CLNIQTGAWILARHFQQCGVNWECLGSYNAGFNKNNTHRRMKYARLIYAAYMQGR"
     gene            19000..19635
                     /locus_tag="ACHLAG_000756"
     CDS             19000..19635
                     /locus_tag="ACHLAG_000756"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFP0654038.1"
                     /translation="MFLIILLVISATLTIQLLAIRLAGHYLMQVHTLRRPRHLSLLCA
                     VLALTATITHIIRGTDIAGLCFLLLMAGCMTLMTLTDAASCRLPRPFTLTFLMLGVAF
                     RLWQGDAAIALACALFWFLLLSLLRRYTSYRKGVETLGLGDVFLIAGVAMWSSANSTP
                     WIIGIAAASTMICMSLFPRSNTGAAIPFGPFICASLYIFTFFPGAIFHTGG"
     gene            19640..21004
                     /gene="pilV"
                     /locus_tag="ACHLAG_000757"
     CDS             19640..21004
                     /gene="pilV"
                     /locus_tag="ACHLAG_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFP0654039.1"
                     /translation="MQKDDDKGFVLFEILAGLIVIGIATPMIYSEIENWLNEQLYQSA
                     AYHADAYNTAARNYIADNNVRLHSGSLPANFTADDLIRQGYLKQGFNHSPFGQSYITG
                     IRRNQTTGRLEALTCSTGGQTIKEEGLRSVAGQLPGLGGFISKNGTATGAFGAWTDKP
                     GDYGLTCSTGHIAVVMSGDDLQESDRLYRFQVAGRPELNQMHTAINMGGNNINNTGNI
                     NGQSATLKGDITSEDGWLITRNNKGWMNITHGGGFTMTDSQWIRAVNGKGITTTGEIK
                     GGKVSGGTVRSDGRLSTGEYLQLDKTATAGTKCAPDGLVGRTSTGAILSCQSGMWAGF
                     ESYPVGSPIPWPSATPPQGYLVMNGQSFSCSRYPQLARAYPGCKLPDLRGVFIRGWDN
                     GRGLDGDRNRHLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAFNYI
                     VKAS"
     gene            21262..21420
                     /locus_tag="ACHLAG_000758"
                     /pseudo
     CDS             21262..21420
                     /locus_tag="ACHLAG_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486835.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            21462..21752
                     /locus_tag="ACHLAG_000759"
     CDS             21462..21752
                     /locus_tag="ACHLAG_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654040.1"
                     /translation="MKHLSSRAATADNNRIVTIEDVYTIAFSQPIHTESALGQRWMLI
                     HTGEYESASDAKRFLDTLVTGHCACMVTPSPVLDHDTWLRIRLLMGEIRDAK"
     gene            complement(21844..22164)
                     /locus_tag="ACHLAG_000760"
     CDS             complement(21844..22164)
                     /locus_tag="ACHLAG_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654041.1"
                     /translation="MKIKQHKEKLEIIRDIIRETLLGNAALIVLAAMVPVVLVKPWDS
                     MTFIFMGAVCVSFAVILFWLVYIVCLYIGTLVKNIFIRIFAFSIWALSAEGAIIYNMI
                     KILS"
     gene            22424..23245
                     /locus_tag="ACHLAG_000761"
     CDS             22424..23245
                     /locus_tag="ACHLAG_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFP0654042.1"
                     /translation="MKPNSTGIAARMMLSIDRARISEALLNNRQLQSTPLQIRYPQGV
                     REALGIMSEQLSLSVSDLTRILVEDALTEMFLPAENIVHRLICRTEHIMQAHDVCVTT
                     MTTLLAPWNIRPAVFRDPDKLTDYLTGEILTALADWFYLNPEWLNGRVRYPLSRAGEW
                     PATQDAFCQLISASDNVDIIIWHSFPFAGVHSQEYCGVLFRKKKEINKAIIYPVLSLS
                     PVRMNKEKENWFHTARNLYAKTSVRTVTLRPAQAEYLMTGKILPAELFSIPLLPW"
     gene            23335..24537
                     /locus_tag="ACHLAG_000762"
     CDS             23335..24537
                     /locus_tag="ACHLAG_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFP0654043.1"
                     /translation="MMMNMHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKAGPEDDFSLLIPSVGGQLSDPGNISDNADKISDDWKAFDRAID
                     SQSNVSQTAARLKTRLQDFRHTHADAQVGTSAVATLPGDTLAAALMLKSYGTVSVDGK
                     VSDADLNYLESIANSNGQDVDKDRLTSQAFARAALVTDVGIALATELETAGQKWSLGF
                     TPKFQRVDLFNYNVLVKNYDSSAFKGDRYHNTRNGINADIGASMDLDDNWTLGLVAQN
                     LLPRSIETKEVNGVTETFRIRPQVTAGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AIGAEFNAWKWAQLRAGYRQNLASNDGSAFTAGLGISPFDVVHIDVAGIAGTDNNYGA
                     VAQLQFTF"
     gene            24677..24847
                     /locus_tag="ACHLAG_000763"
     CDS             24677..24847
                     /locus_tag="ACHLAG_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654044.1"
                     /translation="MFNKNKTHIENKAFMVEPFRDPTANECNVVIANDICIRVALSPS
                     EVLQPFSKNTNQ"
     gene            24896..25348
                     /locus_tag="ACHLAG_000764"
     CDS             24896..25348
                     /locus_tag="ACHLAG_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081199.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HFP0654045.1"
                     /translation="MNRMIPAGCLAVLCLNGCHLPPDNTAPTRKYGAASDVAVARHAQ
                     YQLWGQGIYSAALPERQSGGSLKAHSDRVSFDWEGDALELLNELARLRGQQFSYSGVR
                     LPLPVSLHVRDMTFADVLRLTEAQTAWRATLSQSAGRLHLSFMTPEKK"
     gene            25345..26181
                     /locus_tag="ACHLAG_000765"
     CDS             25345..26181
                     /locus_tag="ACHLAG_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFP0654046.1"
                     /translation="MKTHLCLLLSALLLTGCAGQHHRSSAVMTENAPPPDIRAWLTPS
                     GNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISK
                     EGWLPPVIDEAQDVAHIRPDQIRTASRVWTILRPERFVSNPPGWRDWLLKGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRLNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQMQTVTGNGQKLITGDRVRRLKTHAEFTLQKSRWRPLVSTEGEKNDI
                     TR"
     gene            26165..27313
                     /gene="traJ"
                     /locus_tag="ACHLAG_000766"
     CDS             26165..27313
                     /gene="traJ"
                     /locus_tag="ACHLAG_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFP0654047.1"
                     /translation="MISPDDFGTFPFSRFTAEEFRHFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSVNPLPSSIMMALSDELFGREVLPRVLSGHPVDRTVQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKAISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRHILKNFPDRKIVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVMTDELRNTLSGQS
                     HATWGHYIDEIIRQEKRRIRDQVLTMYQNDRIEKSEAALFIPAGEFPR"
     gene            27310..27600
                     /locus_tag="ACHLAG_000767"
     CDS             27310..27600
                     /locus_tag="ACHLAG_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP0654048.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFMLLGYSGWTVRTLCIRLYSLLQGKQASGRPWWYRHRTELPREWHGPE"
     gene            complement(27777..29390)
                     /locus_tag="ACHLAG_000768"
     CDS             complement(27777..29390)
                     /locus_tag="ACHLAG_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HFP0654049.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDACCQLMTPVNDALYRYVMNTRKVHTDDTPVKVLA
                     PGQKKAKTGRIWTYVRDDRNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAF
                     TGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(29421..29771)
                     /gene="tnpB"
                     /locus_tag="ACHLAG_000769"
     CDS             complement(29421..29771)
                     /gene="tnpB"
                     /locus_tag="ACHLAG_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP0654050.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            complement(29768..30193)
                     /locus_tag="ACHLAG_000770"
     CDS             complement(29768..30193)
                     /locus_tag="ACHLAG_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0654051.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            30255..34220
                     /locus_tag="ACHLAG_000771"
     CDS             30255..34220
                     /locus_tag="ACHLAG_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFP0654052.1"
                     /translation="MRRSVYAYLNGRKAVAWDPEKKLWYAEAGTDLSLLKRWLPRPQE
                     MSMDAGDPVTEFRQVLENAGLVLEKDPEMDGEIHRVPTRDDRRGSQSGAYKAYLDGRP
                     SGWYRDYRSADNSPTTWTFSRAENIDPLARLHLKASAQQNRDEKERKLQQQYNKQAGY
                     ARSYLNGLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFSNGRGEIRSYQRIPVTGG
                     KDARILKGSEKSGNWFAFGTPENGRPLLFAEGYATAASLHEATGHPVLMTIDADNMIN
                     VAKNARKIWTDSPFVFCADNDHQRKINKGVSSAKKAAASTHGEVIIPAFTEAEKAQGL
                     TDFNDLDASRGRDAFQRAMNAQLEYIGIPTPNNDTTDRREAIVIGNLIFTPVKDEKPQ
                     VSPENRQSTAPEPAVSEQNHPYDIPAEETDNQAYEDDYAAYASLQSVTAGTASPNEPQ
                     VNAVSAIDSNAKNTSAATAGDRNVLAQEPNADATVTTTAHDTGTAETDSPSEPAEIQP
                     VQQASPEPSAKDKPQENVVSATDNDAENTSSATTTLRSNDTPETSVSDATGTQHTATT
                     QPEEPQQVSMGSADDWSAFAEDLNADAPGNATVHSTDNAETKTLSEPAGMQPVQQATP
                     EPTEENKPQEEGVMSATDTGEESAAAAIDNDLNTVAEDLNADVTVMTTAHHTDTAETA
                     SPAEPAEIQPVQQATPEPTAEDKPKEERPVSATETSTANETARPETPSLFSRLRRFLT
                     GGKSRTAEPPVIQENKLTEAETATEAAAPPSPPETSPEDTSSAEPDTIIFAPRRPDSP
                     QRHNLDEILKNLTHKEFPDRTALYSVDGEPAFLDRLYCLEMVDGASADDKKVLAALAV
                     ATNFYGGVIELTGSDAFKQKAMQLIVEYDIKVRMKLPAQRAALEKLRKEKEMAHDAIV
                     THRPTPELNRQSPEETTMAQPAPPVSPKAEAEQTAGSTAETPHPEAPVTAKAEAPRAD
                     RPEQPAAPSSAPEKAPVSAQPQQPDTPEEEPPGKLRPGESVTAVLTNYGEKEYAPGKG
                     RCFFVELTNRSGKREYWGNGLEELVKNHQKGDPVTLTLQARETFRLSGETRDRIRNIW
                     AITPVSNGITVNNDQPHAGQHILEFPMKTFSQVMSQMQQHWPQIMQEVRSPKNPPNNL
                     YIGEDRQPASAPQDQSMRLPLAGNAPNELIPAIASADKDNRQLNLLLVHSADDHLQGV
                     VRLNGTLYPALATPSADNRQLVINALTDNGLQFAGYGEAVNHDENTHQRPSPQIMQFH
                     LKQQDSPLFAAIHKPEEQPDKLFRSLGFEQTWKEWSDSQKAEDRQEKTLQQAQSHSPG
                     L"
     gene            34237..34587
                     /locus_tag="ACHLAG_000772"
     CDS             34237..34587
                     /locus_tag="ACHLAG_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP0654053.1"
                     /translation="MNMKKTLLVILTSISLASPAAVAATCRAGAAAEAGSKAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDVSITLPQFPSLDKLLRDLENQVCDAVVDRVNE
                     HLPGNIDPWKDYNL"
     gene            34599..35294
                     /locus_tag="ACHLAG_000773"
     CDS             34599..35294
                     /locus_tag="ACHLAG_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFP0654054.1"
                     /translation="MSEAQPTSPVSPEADNSGDIYNPVLRTIAQSEQRAALVPGLVRA
                     LLCSSAALVISLTGNIIQYWHNSNVTREYFATDNGRLVRLAPTSQPAWSQSDVMTFGS
                     MTLSEAFNLDFVHYRKQITTLAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTATIG
                     AGTLVNQGQMENGIWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAASGT"
     gene            35305..36279
                     /locus_tag="ACHLAG_000774"
     CDS             35305..36279
                     /locus_tag="ACHLAG_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HFP0654055.1"
                     /translation="MQIHHLLGALLIVCGTVTAADNPGWQNARTPQAGGKTGQTPGSA
                     PAGGPPPATQLTGPLPPPASPLVQEAASLDSPLNAGEIRELRGRMADNERAISAPVTS
                     VVPRISSLTVNLSPGASLPLVRTALHNVSVVTFTDINGSPWPQSDPPYNAAPGHFDVQ
                     FNGNMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTNTLSQEIDSRLDLRIPR
                     QGPASPVVAVPSEKIALHDATLQAFLDGVPPDDPTVRRLKFSGNVPDTTIWQHGDDLL
                     VRSRAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSIHVTPELE"
     gene            36283..37614
                     /gene="traO"
                     /locus_tag="ACHLAG_000775"
     CDS             36283..37614
                     /gene="traO"
                     /locus_tag="ACHLAG_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFP0654056.1"
                     /translation="MSAEQDTAAKSGKKLSALLVLGGVIIIGGGYLLLTWLSPENSAS
                     LSSVNINSTTTGSGRTVVESQHYRDLLRADNERGAAEALRNNQTFIASLPRGLDTDHG
                     KNQRRDTQPADQAEGSGQPSTPPQADQTAREKQREALQKLLARINTQHPDARPPVVAA
                     AMWGTTGAPGISSHASPPGTGTSPQPRLQEASLATPAARTGIQLIPALTRVPAFIDTE
                     VDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGKGMDIHFDRMMWRGMFLKVNAYALN
                     EHTGMSSVASDVNHRWFKHIVLPSVLGGVGSVGSLYKDANTQVIQGNYGSITGRVGMP
                     DGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDISPPS
                     SGTAPSVPPPSGRPLTQEEAERQASDRIQAAVIRKQNEMRQRYGIQQESHP"
     gene            37611..38324
                     /gene="traP"
                     /locus_tag="ACHLAG_000776"
     CDS             37611..38324
                     /gene="traP"
                     /locus_tag="ACHLAG_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFP0654057.1"
                     /translation="MKNEHEDPGSFGETTTPASGKSFLKRQYMGYSLPVWAGCVLIIL
                     VAVWYKFLRTPSEPPADAGFAVTPPAAESWAEPMPMKETGSGNTMSAPPVTPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKESITTLGDTIRKDEAWAQETRQLLDETRKQVHAL
                     QMQGPLQIPTGNNRQPAKTPPSRLQGMKIMSVEKGMAWIRWQGSAWAVREGDRLGNIT
                     ITRIEPSSRSVITSAGTLR"
     gene            38321..38851
                     /gene="traQ"
                     /locus_tag="ACHLAG_000777"
     CDS             38321..38851
                     /gene="traQ"
                     /locus_tag="ACHLAG_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFP0654058.1"
                     /translation="MMSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIVICGMLVALPSFINAGGTQMGFRADSFGPIAYVQPTTFGAA
                     AGAANAMLSLVKLAGVGFAMNGISIWRKSLLDGHTALSSSESISKGNVKFVAGVLLVF
                     IDRVIDASLASLGLAF"
     gene            38900..39298
                     /locus_tag="ACHLAG_000778"
     CDS             38900..39298
                     /locus_tag="ACHLAG_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HFP0654059.1"
                     /translation="MSRFRAMTARLCAAFIYVQLYLVACRDRLIRLLLLLPVILLPHT
                     AMANTLFDMIKTVGDGAKGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGLCLAVVPELMGSGQKTLGLSPVSIS"
     gene            39355..39606
                     /locus_tag="ACHLAG_000779"
     CDS             39355..39606
                     /locus_tag="ACHLAG_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HFP0654060.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKTYEQNTATHRPGPGTLAFPAPATGHGIQR"
     gene            39521..40339
                     /locus_tag="ACHLAG_000780"
     CDS             39521..40339
                     /locus_tag="ACHLAG_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HFP0654061.1"
                     /translation="MNKTPPLIAPVRERWLFPLPQPDTVFSGESLLPPPVLSTPGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTVVLLEKNLTRVMRLKKHPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESELLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALKEIFSDERKRIRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRQMLREKMDGMALILLPDSFPDKKYVM"
     gene            40394..40753
                     /locus_tag="ACHLAG_000781"
     CDS             40394..40753
                     /locus_tag="ACHLAG_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654062.1"
                     /translation="MDTLTAGYQFSADAFRHFIIRTLTDDIPPDGVEKEIARFILRHP
                     AFNGGWLLFLAAEIHERSRTHNSITVTVSVDNYLSWMDIAVSGLPELPDMPFCDFFIR
                     RQQFIYPGVPPTKSGEE"
     gene            40753..41133
                     /locus_tag="ACHLAG_000782"
     CDS             40753..41133
                     /locus_tag="ACHLAG_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654063.1"
                     /translation="MKIHRISPETLITLILAHLAGKADSTAKEEHRLLRRFLRNDDGR
                     LAGILLNIAGILQFNRELSARHNYPATPLTEFSLRKRGKQLHLCLCSLRFFYIPPVFI
                     QNKRRKSIVVHLNKITYKQTHSIR"
     gene            complement(41106..41651)
                     /locus_tag="ACHLAG_000783"
     CDS             complement(41106..41651)
                     /locus_tag="ACHLAG_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654064.1"
                     /translation="MKAENKILNLFHATTMSRGLQIIKDGKIRTNAPSVICWSLETTP
                     GWIYLSDQLDNAVHWGNKIACLSKDELFFYIFRVQVQEENCYPDTDDLQYVSWLSPEE
                     AENTDLYTCLSLCHSCRTDKELYTGCEISDYAVLPATTLYTDNSGHPQYDIVNTLKKH
                     IGKDEYNQYESLRQRIEWVCL"
     gene            41876..44920
                     /locus_tag="ACHLAG_000784"
     CDS             41876..44920
                     /locus_tag="ACHLAG_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP0654065.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDLTAPGTLESLITALAERMNTAY
                     KNIGHKISFVFERDPERGRNEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRIRKLAEHAPPARFAQDPWRWTLSALKIRHDAFLD
                     TLEQALTHDSDGLLVRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGED
                     TSVFHAPSLNLQLFTTQPETHGSLIKAGDLWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPGGMKALGVKKTLLTYSSFISPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHGSGPVPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PEHTRNLFDVMYGAKKPATPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISNLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHMAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSAELLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQDYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQEVGSLRARQI
                     LAEHFPRGSATSLIEHRARTHDSENVIHELAAELIRKQGYHL"
     gene            44920..45537
                     /locus_tag="ACHLAG_000785"
     CDS             44920..45537
                     /locus_tag="ACHLAG_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFP0654066.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGIRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     APLTALMSDNTWLWPDSPGTPALTAGDWPVMETDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESHSHRVPDILYPLKTEKKECKDNQQPHCLHS"
     gene            45498..46703
                     /gene="traW"
                     /locus_tag="ACHLAG_000786"
     CDS             45498..46703
                     /gene="traW"
                     /locus_tag="ACHLAG_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFP0654067.1"
                     /translation="MQRQPATTLLALLIGCTVIPSTHAYPVTVVASQPVTTQIMPELA
                     AIQGVMAEMVRMQTATGTAINQSSEKLASVIAQDGQATRQQMIFSNETRRLEEARKSF
                     SVPDSICSESASGVAAESRRAAASASARLSQGGGVSSKPIRERLSRAADSPTREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGELPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLSELS
                     QAMSQGSVR"
     gene            46700..47269
                     /gene="traX"
                     /locus_tag="ACHLAG_000787"
     CDS             46700..47269
                     /gene="traX"
                     /locus_tag="ACHLAG_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HFP0654068.1"
                     /translation="MRDGNSTDEKKTATRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     GQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVNELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPAITLIRILACLLLMSAIAALCASKTLIVSYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            47342..49507
                     /locus_tag="ACHLAG_000788"
     CDS             47342..49507
                     /locus_tag="ACHLAG_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFP0654069.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASNNTIIGNLFMVFNAIIAIMGIVWFLFIGIRHVVRTGHQGNVFNN
                     GRDVVGMLSIVSGFMMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMALCKHAMNAGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLTVKGNGSNNQSALDYFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSSQGEAGDTEFLKEVKGAYQ
                     AQLQNSPYTPALGTATSASEYQASNTQNPQSALLKLLGNDLTLFTNQIATIHSGTGTT
                     SDQLNPLIKMKNIGDYTMVTTETIWAGYTTVRVLSTAGSNSIFGRLVNAVSGLSDAAK
                     AFLEASAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGVGNWIVSVLIGCAAGPLWA
                     ATHLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIMAGGTILNALFAAALM
                     NVQINSVTGVFSMAGFLMIYARVCTTLVAAVFALQAYLPDHVINFLGGRDGVNMLGNM
                     VSSVKDIIAGGNTNMRRTPGVREEKLKNINGDDKDGIKQ"
     gene            49595..50245
                     /gene="excA"
                     /locus_tag="ACHLAG_000789"
     CDS             49595..50245
                     /gene="excA"
                     /locus_tag="ACHLAG_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFP0654070.1"
                     /translation="MNKTKSRYPSWWAYYQLARNAYFLLGIPCLALYATAYTSYLLDD
                     KLTHSTSLFILAYGCYFLLFIPPLWLYLRTRAKKKKVQQVVQQIKESSPFAPTSDYEQ
                     LSFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSL
                     PMIPLEISGISSRTLANTMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            50519..>50777
                     /locus_tag="ACHLAG_000790"
     CDS             50519..>50777
                     /locus_tag="ACHLAG_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HFP0654071.1"
                     /translation="MSESRSNFPARHGTPWNTEELQRLLVSIAEGVSLKKLTQTHQRS
                     SGGIRGAIKRLLPSEFLEFNDINTVNDLSNYFNKIPRQEHNS"
BASE COUNT        12699 a        14111 c        12569 g        11398 t
ORIGIN      
        1 attattcttg ctgcatgtca ggcaaactat atccgcgatg ttcagggcgg gactgtatcc
       61 ccgtcatcct cagctgaact gaccggatta gggggctgaa gtccaatgga acgaaaacgt
      121 acgttagtga agtaactgtc tgatatatca aaacataatg gacatagaaa aacgccatca
      181 aaacggtgcc tttttaatcg aaaattggca cttaacggac ggcattgtct actaatcgat
      241 ttttaagacg ataattttcc tcttaccgta cgatttcgga caccaaaaca gctgttgtcc
      301 cgccatccta gcgattcctg aaagtcattt gtctaaaaaa tagtcttaac gtggtttatt
      361 ttccgaattc caagcggccc cctggatgag cgtgatgatt actggcggac ctttgacgtt
      421 ccagcaaatt cccctgttcc acataggttt ccagatatga ccggttattt tcacgttcgg
      481 tgtacgtgtt acagctgcaa ataatacagg tgcattatca atatcatcag ggataaaaat
      541 gtaagatcgt ggaaaatgtt tattcatagc ccggatgatg gctgtctgtg catccattgt
      601 tcgggcgcgg tgtttccatt cacgccagcc atccacctca gaaataataa cagtaatcat
      661 caaaacctcg tgtcgttact caattctgat tccatagcgg gacagcattt cacataatta
      721 tgccccgtta tgaatgcagg cgtccttcaa attgctgtcc ggtgtgacag tttgtcgatg
      781 gttggttgca acgagcatct tctgtctctt tcatcctggc gttataacgc tgcgtgtgtt
      841 tttcaagttt tgcctgcgca tacagcgctt cttcctccgt tacagttcca tgaactttgc
      901 catccagacc ataacgcgga gtacctgcga ctgatatacg ctgcagatat ttgtaaccgc
      961 cagtaatcat ccttacagca caattccatt cttttacagt tagtccacac tcaggactgg
     1021 ccgaaataaa agccatgaca tcctttgaga tgcctatttt aaatgcatga tatcccccct
     1081 cttcagcccg taactgaggg aatgtttttt taacacagtc cagactgtgt cggataaact
     1141 catgattcca tctattgttc ttcttttttt tcttcttctt acgtcctgtt tttttctgtt
     1201 gttccgggga accggccact gtggctggta tttcaggagg taatgagtcg gttttaaatt
     1261 ccattactgg cgctggtttt atatcatcag gcactgcctg tacggcatca tcctgatgag
     1321 aaagaattcg tctttttttc ttaataataa caacaggtga cggtttcttt gtaacactgc
     1381 ccatatacac acctctgcgt atcgttaata tataatgcac gacatacaga tagtatatat
     1441 ctgagtcggc aaagtcacca acagaagaat gataacatgt atttcattac tgttcgcgaa
     1501 ttatcactct ggattccttc ctggtgacat aagtacagtt atcaggtaca tcttccataa
     1561 taaaagacat tgccccgatt ttaacattac ttccgatttt cacatttaac ccaataatac
     1621 atgtatgggc accaatatcg acattatcgc caataacaat atagttttcc ctactgtcag
     1681 aaaccttttc accaatggtt gtattctgcc gtataatgac attctcaccg atatcaacat
     1741 taccatgaat gacgattcct gcatgatgag gtaacatgag gcctttacca atccgcgcgc
     1801 ccaggccgat atcacatcca taccgttcca taagtgcatt ttttatcttt atcgcacatt
     1861 tcctttgttt ttggtttcca gagataaaca tctcattggc caggcgccac cacatcagaa
     1921 agcctcgtta tctctgcaac ccgtttctta tcaggcggtg taagacaaac ttatcccgca
     1981 tgatgacttc ggctttccag tattctgaca gattacgact tttacctgac aataaaaagg
     2041 tggcaatatg aaaataagct aatggatttt tcatcataaa aacgacctta aagaataaag
     2101 aaaatattct gtattgtgga ataaaataac ataactatca gaaatacttt taaagtaata
     2161 tcagtcagct ttaaagataa ctatctcatt ctctcactgg cgagattata aacgttacta
     2221 cgttcacgtt tacctaccgc gacaaccgca ataattagca tgtcatcaat aacctgatag
     2281 accagacgaa accccgatgc gcgaagtttg attttgtagc agtcttttaa tccacgcagt
     2341 tttgcggaag gaatatgagg attttcgctg catttcttta gttttttcgc aaactgctgt
     2401 tgaatggatt tatccagttt cagccattcc ctgagggcat catccctgaa tttgaccgta
     2461 taagtcataa ataactgtcc aggtccacat catgcagtgg ctgattgctg cgttcagtaa
     2521 ccagtttcac cagctcctgg tcatccatag catcaagcat cctttcatac aattcagcag
     2581 gaacacagta gaatgcaggc tgattccggt taagaatggc aacaggatac ccctctccgg
     2641 cactgactgt cgccattgga ttttttttca gttcactgac gcttgcactg gtgtcagaca
     2701 aaatgatgtt aggcataacg tacctttgag actgtttaac aagtcttaaa gatacctctg
     2761 aaaggaccgg ttaacaagtc tgtattactc atacttttgc aaatacattt aatgctggtt
     2821 ttattgttct gatgccgcgt gagcggcatg gaaggcggca tgatgtaact acatatggca
     2881 caagagtaaa accgccgaag cccggcgtaa gccggtactg attgatagat ttcaccttac
     2941 ccatccccag ccctgctaag ccatacccgt tttcagccat gacagagctt ctgtgcgcgg
     3001 tcggagtggt cccgacgagg gtttacccga agtcggggcg tatctccgcg ttagcgggcc
     3061 gtcagggccg cttacgagcg tgtactgagg acttccagcc agaagactga cagcgacgag
     3121 gattcttagt tacaacattc ataattaaaa gcgatgttgt tcccgccctt cgggcggggg
     3181 cggtctcgct tttcagttat gaggggtggg cacggtgctg cgtttgtgtg gtgtttttat
     3241 tcctgatgtc ttttttgttg tggtttcaga acggtgaggt tcggggcccg cgcgttagcg
     3301 tgggaggtgc atatttgtct gtccacagga caggcactga ataggtcttt tttttaaatg
     3361 gaagtaatta agtagtttaa aggaaataca gtactggggt ttaaaagaaa cattgcaccc
     3421 tgtaacgcct acggctggcg ctttatagca tgaacggttg taaccttacg gagaagtccc
     3481 ttgcagttaa atgtggataa acagaattcc ccgtctgtga gacgtgtttt gcgtgaaaaa
     3541 cagggggagc aaggcgagcc ggaaggcaga tgatgaggct gttttttgtg tgctgtgctg
     3601 cttttttgtg cgtcgccggg cttcaggcgt gcggatgcct ccggcgcagg ccagattatt
     3661 cagatgaggt caaactcagg gtgaggcagt ggccagccgt gtgtagttgt tgttccgcga
     3721 cattatcatg cgttccttca cgcgacggtc gatttcgcgc ttcacggcct ccaggcttcc
     3781 gcggaaacgc ccttccgcga tttcacgtgt cagctgccgt ttcaccaggg tgacaatgtc
     3841 ctgacgggtc ctgcctgcat cgcggcgcgc acgggcacgt ttcatgccgt gggacttaag
     3901 ctctgcctga taactgcgga aacgctcgcg cacaaaacgc caggctttcg ctatcagttc
     3961 atccataccc agcgcatcca gcccctgctt tttgcgcagc ctgttttccc attccacgcg
     4021 gctgcggcgt gcggaagcca ctgcctcttc tgagatatcg agtgaggcaa acagtgccgg
     4081 cgtgaacgtg atgtcggtcg ggatattaca gccaattgtc gggtcgtatt ccgtctggta
     4141 ggttatcagc cccagctcag ccaggaactt cagggcacgg gtggcacggg tgatggagag
     4201 tttcccggcc tcagactccg tcgccagtcc gcactcaatg gccagcgtgg tgatggagca
     4261 ctgcacgcgg ttggccagcg ggtcatagtg gaaacacagc ccctgcagca gcgcatcaat
     4321 cgcccttcgg cgcagcaccg gcggcatacg ccggcgcagg ccgcgcgaac gggcatacgc
     4381 cacatgcgcg gcaaaatcaa aacgggaggt gaagcccacg gccttttcca tcagtttttc
     4441 gcagaacttc agcgttccgg ctccttcacg gggtgtgaac accggattcg ggttctttac
     4501 ctgacggtat gtgctttcag tgaatgaggt ctggttcagg gccgccacaa gctgtcgctt
     4561 atggcgggca cagaatgccg tgcgttaatg cactgcaagg ggttgaatct ctgacacggt
     4621 ttcatatata cttatcccgt attctgtgag gcccccacta tctttcttga gcccgccaaa
     4681 gttcatcgaa agatagtggg ggttttcctt tatggtcttc tgaaactttt ttcagccttc
     4741 cggccccggc aaacagccgt cgtttctgcc atacatcctg aatgccttat ggcgcatcct
     4801 gccgttttct gactttccgg accgcgagga tcccaaaagc cactgaataa cgatccgcaa
     4861 tatacccctt ttccaccgct ttgaaaagcc gacacgattt ctcagggtta cagttgccct
     4921 ctttaacccc ctaaaaagcg tcgttttttt gaactctgtc aaaaaaatga acaatttcac
     4981 aaacgatctt ttatgctgtg tttttcatgt tttttggatg aatcgatctt gtaattacga
     5041 tctacgatct aatcatccaa acacctcaca caaaacagca taatatgctg atttatcaaa
     5101 tataacgatc tattcaactg cttatcttcg ttcttgccac agatacatcc ttatgtaata
     5161 tatttgccag ccattaatcc tgtttgcagg agttgtcggt tttcgcccgc ctcgaaaccg
     5221 tccacaaggc ttacaagagt gcgtaaatca tgtgcctgtc agaactgcag gccgggctga
     5281 acgagggtca gccgctgttt ccgacacatg ctttatgtat tcgccgttca caacaacatc
     5341 gaagccccct gcggggatcg attttcgcgg agcgttccgg attttgaata tcagtgaatt
     5401 acatcaacgc aactggtatc tggcacagta catccctgcc ggcaaaaacc gcgaacatct
     5461 cttttcctgg ctttccgaac aacacgtcct tccctggaca cccctgatac tgaaaaaggt
     5521 ccgccgcacg gataaagtct gtggttaccg tcgacatata cacgccgtgt ttcccggtta
     5581 ctttttcctg aaagcagacc ccgaacgcca cagcttcact caccttcgcc gtcacagtgc
     5641 ctttctggac ttcgtgaaaa tggccggtga aatcaaaacc gtcagggagg atatcgttca
     5701 gtctttgatg aaagtgtacc cggatcctgc gctcaatccg gcagccaggg aggagctgga
     5761 tgctgcctcc accctctggc tgacaaaagc gcgttaccag tacctcctcc ggcttgatgc
     5821 acagcctctg ccggagtcga ggatcgccct gctcctgcac ctggtatctg atgacggagc
     5881 gttgacatga aaattgctgt cttcatttga cgactttccc ggcctttatg gccgggcttt
     5941 ttttccccat cttttcccca atgcccggca ccggtgacac ctgcgtgtac ggagagcggc
     6001 tgccgggcat tttttttcag gacatcgtta tgaccattca atccccggaa aaccctgtca
     6061 ttcagttact ggagaatatg cttgaagcgc tctccacctc cccggacagc atcaacaatg
     6121 aacgacgtcg ccggcgcttc ctgctgaact ggcttgattc cgtccgacag atgagtgagt
     6181 tcagggggat gcctgaagag ttcaccgccc tgcgcaaact gctggccacc aatacggaca
     6241 taccggtaga gaacacgctg aaatcactgc tggaaagtgg ccacaacgca gcactgtatg
     6301 acctgtaccg cttccgagca gcgcttgcca cgtgcaggag tctgggatgg cagattggca
     6361 cgtgcgcatg gaatgaccag ttactgagtg agacactttc ccgcgcacag accgggaaac
     6421 gtcatatcct gcagctgaac agcaccgggg acaccttttc caccacgggc agaatgatca
     6481 gaccggtggc attttcatta atccatccgg ccaccatcaa cacgtctgag gttgaagaga
     6541 tattccggga tgaaggtttt atgctcgcac acgggcgcga atgcagtctg aacggcagcg
     6601 gcagaaatat gctgtcacgc attcttcatg tgggccattc aggtttagcg gaagaggaat
     6661 ggggcatgga caacagtctg cttttacact gaaataccgg gacagccaca acggcagcca
     6721 ctctccgctg actttttttc gcaaatcgcg gtttgctgat gtggatgtcc catttttgat
     6781 tatccgtcct ctccggttgt cataaagcgc attccggcgt gaggcttctg ttgttatcat
     6841 cccctcctgc agacatcccc ttgtcttctg atgactgacg atatgcttct tctgacaatt
     6901 aaagaagcgg aagcttcacg ccataatgtt gtttgactga cctgccacag gaggaaaaat
     6961 gacactgacg ttacttaccg cgtataacgc accctatctg gcagccctga cttttgttct
     7021 gcttactggt atcgctgaac ttatcgccct gctctgcgga cactccctct cttcagctat
     7081 ggatacacct gatttaccgg agggtctgac aggagaggcg ctggactggc tgaacatcgg
     7141 acggatcccc ctgctgatcg tcctctgtat gctggcaggt ttttttggta tatccggcat
     7201 tctcctgcag ggtcttatta ttcatctgct gcaggctcct gccccgaaca ttctccttac
     7261 accactttgc ctgctgctga catgtcctct cgtacaccgc accggcagac tgattgcccc
     7321 ctggttaccc cgtgatgaaa caacggcgct caccgaggag gcattcatcg gctgtatggc
     7381 caccatcacc ggttatcacg cagtggcggg ttccccctgt gaaggtcgct tcacagaccg
     7441 cttcgggcag gttcattacc tgttgctgga gccggaggcc gggaaagaat tcagacgagg
     7501 agacaaagtg attattgtct gccgtctttc cggttcccgc tggctgggcg aactgaatcc
     7561 ctggccggat gttctttaat cccgacatat acagaggttc tcactatgtt taatcttccg
     7621 ggatggttgt ttaccgttat agcgctggtc ctcaccctgt tcgttatcgg gctgatattt
     7681 gcccgcctgt accgccgggc atcggcagag caggcctttg tccgcacagg tctgggaggc
     7741 caaaaagtgg taatgagtgg cggtgcaatt gttatgccca tttttcacga aacaattccc
     7801 gtcaacatga acaccctgaa gctggaagtc agtcgtgcag cggctgaaag tctcataacc
     7861 cgcgaccgca tgcgtgttga tgttgccgtt gccttttttc ttcgggtaaa accatcagcc
     7921 gaaggaatat ccactgcagc ccagacgctg ggacaacgca cgctcacacc tgaagatctg
     7981 cgtagtctgg tcgaggataa atttgttgat gccctacggg ccacggcggc cagaatgagc
     8041 atgcaggatt tacaggatgc ccgcgagaac tttgttcagg gtgtgcagaa caccgttgcg
     8101 gaagacttat ccaaaaacgg tctggagctt gaaagtgtgt cgctgacaag tttcaaccag
     8161 actgcacggg tacattttaa tcctgacaat gcctttgacg cagaggggct gacgcttctg
     8221 acccaggaaa ctgaacgtcg tcgtcgcgaa cgcaacgaag ttgagcagga tgttgaagtg
     8281 gccatcaggg aaaaaaaccg ggatgcactc tcccgacgtc tggagattga acagcaggaa
     8341 gccttcatga ctctggagca acaacaacgg gtaaaaaccc gtacggcaga gcagagtgcc
     8401 agtattgctg ctattgaagc tgaacgacgt cgtgaagcag aatccgcgcg aattctggct
     8461 gaacgtaaga tagaggaagc cgaaatcgaa cgacagcaga tagtgcgtac ccgccaggtc
     8521 gaagccgaac gggaagtggc catacgggaa attgagcaac agcaggcaac cgaaatcgcc
     8581 agccaggcca gagccattgc cgtggcggca aaatcggaag agcagtcaca ggcagaagcc
     8641 cgggcaagta aagcactggc tgaggccgta caggcccagc aggatgtgga aaccacccgc
     8701 cagactgcgg aggccgaccg taccaaacag gttgccctga ttgctgctgc tcaggaagct
     8761 gaaacgaaag cggttgaact gacggttcgc gcacaggctg aaaaagaagc agccgaactc
     8821 caggcagcag caattattga actggcggag gccacccgtc agaaaggtct ggcagaagca
     8881 gaagcacagc gcgcactgaa cgatgccatt aacgtgcttt cggatgagca gaccagcctc
     8941 cgcttccgtc ttgccctgct ggatgcatta cctgccgtca tagagaagtc cacagaacca
     9001 atgaaatcca ttgacggcat taaaatcatt caggtggatg gcctgaatgg cggtgttacc
     9061 cgtggtaaaa cagcaacaac cgacataagt ggcggaaatc tcgctgaaca ggcggtaacc
     9121 gccgctctgg cttaccgtac acaggctccg ctcatcgact ccctgctgaa ggaggtgggg
     9181 ttatccggtg aaggacttac agccctgaca cgtccactgg tcatgccagc cccggaaaag
     9241 gtcccggatg tcagcgaacc ggtaaccgct ccggataact cagaaccaca acaggtttaa
     9301 tccccgtgcc ggaatttttc cggcatcttt ttcacaaaga atgccctcac gggatattct
     9361 ggtttaaaag aggattgaaa atgtccgcag aaaaattcag agttgtcata tacaccaata
     9421 catgtgaaaa gaaagtcagt tatattcctg caacagaaga tataaacatc gctataatgc
     9481 catttcagac ggcagacaat ggcttaatta ttgtgtgtca gaactcccgt ataatacgac
     9541 gcaaagagcg tatacccgga agcagctgtg aatggaaaga tgtgacatta gcaccaaaat
     9601 ccagaaaccg cgttcagtta tagcctgaca gcctgctttt tatccctcag aaaaggggga
     9661 taaaaattaa atatttcaca accgagatat ttttcaggaa tgcagattat tttatgttat
     9721 gaccaacaga gaaacaggca tgagaacaat cacaacgcat gaacaattat taattaatgg
     9781 gagagtccac gaaagaatcg ccacacatat agttacagga gctcatgggt atgaaacgct
     9841 ttgcacaaat ggctataacg tcagatacaa caaggcgcag caactggttg aaagctgcga
     9901 aaaaatagct gaaggcaaac tacctgttac ctgtcctgtc tgtttcgcca tatggcagga
     9961 tgtacacagg ttcacccacg ttgactttga cacacaatcg gggaaaagtg attttattga
    10021 tacctgccat tcggagatca taaccggaaa tgtttcaata acaggcaaga aataaaataa
    10081 tgctccagtt ctgaaatgca attcactctt ataatccccc cacgaacatg aacgttcatt
    10141 atcaataacc cgatgaaatg atattcatct gaaggccagt tacccgccac ctgtcttata
    10201 ccgtaacccg taatataccg gattttattc tatgcaaaaa aaatcactta cactccttct
    10261 gccgtcatta ctcagcggct gtacggcatt taatgccacg cctgttcctg acaccacacc
    10321 tccggctctg gtgttcgtcg acggacaaat tcgcgaaagc gctgccatta tcgcccatac
    10381 gcaaaaacgt ctgcttccgc caccggtgac ccgccctgtg acgccgcccc gcccgacaac
    10441 aacagcgacg cccacaaccg cagtcagcag ccagagtacc gcactgcctc cccgcccgct
    10501 ccttcaggcc acacagctgc ccgcactggt tatgaccggt aaacccggtc agtcatccgt
    10561 cgtttattta cgtgaggcac gtcagcgctc cgtcagtgac tggatacgcc agttgctacc
    10621 acccggctgg cagttccggc aacagaacgc tgccataccg gcactgaaca aacgcctccc
    10681 cgtctggtcc gttaatgacc agtggcaccg ggcactgaat actctgctga ccgggcagca
    10741 actgtacgga catatggact ggaacaggaa aatactgact gtcacgacca ctgcaacacc
    10801 gcctgttgac ctcacggcac cgcagggttc acagaaaaca gcggacacac cacgtaatcc
    10861 gttcaggagc acaaccggaa cggctgcaga ccaggcacag gtaccacctg ccgtcagcgc
    10921 ttcccgggtt cagagcacaa cagggacatc cggtacatct gcgccccgca caaccacgcc
    10981 agccccggtg gtcacgaaac cggtagcacc accacagaag gtcacaccgc cacggcagtg
    11041 gaaaatggaa aaagggacca cgctgaagga cggactggcc cgctggacgt cgtccgaaaa
    11101 atgctcccac ggcacctgga cgctgcgctg ggaaacgccc gttaactaca gtatcgatgc
    11161 ccctctggtt ttcaccggca gtttccgcca ggcgctggaa cagctcttcg cactctacac
    11221 cccggtggaa aaaccgctgt atgcccggac aagcacagca caatgtctgc tgcgggtgac
    11281 agatcgtcca caggaataac cttatgggct ggttcatcgt gttcatcatg gtcatcacga
    11341 ccacaggcaa tttctggttc accagtcagc tctcccgcag cgaacaccgt catcaggccg
    11401 cagaaaacac acaacaggca gcaacgttca ttcgttatat gaacgccatc aatgattacc
    11461 tgcatcagca tccggaacga cggacagccg gagggcggct gacatccgct cagctcggga
    11521 ttcccgggac ggataccgtc agtcatatca tcagccagca acgtgttttt gtctgggcta
    11581 cagaaacccc gggcctgatg gccgcgctca gggaacagag taatgactct gctctgctcg
    11641 gcagggtgga aaacggccga ctgctggaca cagccggcag agcgctttcc gtcacacttc
    11701 cgtcagtgat ccccgatcac gtcatcctct ggatgaatta gtcacgaatc tgaataaatc
    11761 acggatctcc catgaataaa aaaattcttg ccattatgat tgccgccgct ctgttacagg
    11821 gctgtgccag ttacagacag cttgagagca caagaaagga agccgttgcg gaacaacaac
    11881 gggcacggga aatgatgtct gatctgcgaa cccggacgcc ggttgttaag gaatcctcac
    11941 gacaatggat caatccccgt ccggtcatgg ataaaaacac cggcaaaacc gttcccggtt
    12001 gtcccattgt cattcatacc cgtcagagca tcacccttca gcaggtggtt cagcggatca
    12061 cggaaagctg ccatattccg gtacggatca cacctgatgt actgacatac ctgaatacat
    12121 ccacaaccgg cagcacacaa caaatccagg gcgcattacc cccaccggat gcgagcggca
    12181 tggttcccct tgccgccatt ggcgcatctg acacgccacc atcccatctg ccgggaaata
    12241 ccgcagcact gtctgaactg aatgcctccg gcctgacgac cctgctgaat gcggtctcct
    12301 cccgtctcgg tatatcctgg cgctatgacg gctcgcatat catcttctat tatctcgaga
    12361 cacagacctt ccccatcacc tatatggaca gtcaggtggc ctataacgcc actgtggtca
    12421 gcggcaccat gtcctcaaac ggctcatcag gaggcagtgc atcagatgcc atttccggtg
    12481 atgccagcaa tacccagacc accaccgtgg atatgaagtc tgcgctgtat caggatgtga
    12541 aaaatgcggt cacatcaatg ctgaccccgg gtatcggacg catgttcatg tccacgggct
    12601 ttatcaccgt cacagataat ccacaggttc tggaaacggt ccgggcattc attgaaaaac
    12661 gcaatgaaga gatgaaacgt caggtggtgc tgaatgtgga aattctgagt atccggaaaa
    12721 cccgcaatga gcaggccggt atcgactgga acgcagtctt cagcgacaga acgctgggac
    12781 tgagtctcgg cagcacattc accagtgcag ccagtgatgc catcacaggt ggtgtttcca
    12841 ttgtggacgg taaactcacc ggttcaaaag ccttcctgaa agcattgtct tcccaggggg
    12901 atgtcagtgt ggtgacccag aactctgccg tcaccaaaaa cctgacgccg gtgccaatgc
    12961 agatcgccaa tcagcaaagt tacatcgaga gtgtgacgac ggataccaca gccaatgtcg
    13021 gctcatccac ctccctgaat gcggccacca tcaccaccgg ttttaacatg acccttctgc
    13081 cgttcattct cccggactca cagacactgc agttgctcta ttccatgagt ctctccgaca
    13141 aaccggttat tgagaattat gagagcggtg gaagtaaagc ccagctgccg aatgtggatc
    13201 tgaaaaccat caatcagacc gtggatctga aatccgggca gacggtcatt atcagtggct
    13261 tccagcagtc aggacgtcgc agcggaaaac agggtgtagg cacgccggga ttcttcggtc
    13321 ttggcggtgg gatcaacagt gaaaacgacg acaccattct cgtggtgctg atcacgccca
    13381 atatcatctg atttgcacaa ggaggacgat cgtgacggat atttcaccga cagggcggca
    13441 ggtgtttccg ctgacggcag ggcgcaatgt gtttctggcc ggtctggaat ggaaaacact
    13501 gccctcacag taccggcatg cccgcgactt tgcccgggca cagaaagccg accttttcct
    13561 ggcctgtcag tatctcagta atgaagatgc cgatacccac accatggtag ccactgtcag
    13621 tcgccgcata ctgcccggaa aaccccgtca gtgcttctcc ctcgccctgc tgatcctgcc
    13681 actacttgaa catggcggct atgccatcac ggaactgacg ttacccggag aaacaccgca
    13741 ctatagcttt gtttccgttg tcgacggtgt tctcgtcagt gacctggtgg gaagcgggga
    13801 agaggttcgg gaagcccggg atacctttct ttccatcaac accgaaccga ctcagggctg
    13861 gatacactat gaaccagcgg cattttcagc aggcgctcag aatcagcacc tgccgctggc
    13921 ctccctgact ggctctggta aacatccggc tgccgcacgc ctgaatccgg tatcccgggg
    13981 accgcagttc atcactgtct ctctgatcat cgcccttctc ggcactgcat ggtacggctg
    14041 gcagtattat gaacgctggc agacagaaaa agccgcgcag gcagcggttg cccgacaggc
    14101 cacagaggaa cgaatcactc ccccctggcc cggactccct gaaacagacg cgttcatccg
    14161 gagatgtgct gatatatgga cttcactgcc actgtctgct gccggatggc gttattcgct
    14221 ggcagaatgt tcgactgacg gtggcagcgg caatcttcgc gcatcgtaca ccaatactgc
    14281 gggcgcgaca gtgactgact tcatccggcg catcagtgat atgacagaca gccgaccgtt
    14341 tattgtgatg ccggaaggaa agaccggcgg cattggcctg ccggtcacct tccggctgtc
    14401 tgaaaacgcc gtcacagttg atgcactacc ggaaaccgct gtcctgcagg aacgactgac
    14461 ctcgctggct cagtcaatgc aactgaaact ggactggcag gaagttaata acagcgtcac
    14521 tgatgagaac ggacagatca ttcagccccc atggaaagag tatgaccttc agatacagac
    14581 cacactccct gcacaccatc tggctgaacg cctgaaagag ccttctgtcc gttttatctc
    14641 tgtcacacgg caactggaga acggtcgttt ccactatcaa tttacaggta aatattatgc
    14701 ccgataaagc caaaaccctt ctgttttcag tcctgctgtt accggccacg gcaatggcca
    14761 caaccggaaa tgcagagcaa cagaacacac agcaaataag gattgaacag atacaggaag
    14821 agacagccct gtatgaagcc atgctggcaa gagagaagat ccgccatgaa ctgcgaacac
    14881 ttgagcaggg ggggcccgca gaggccggac gtcagcagga aagcatttcc gcagtcacaa
    14941 caaatacaca ggcatcacta tccccccgcc tgacagcaat attcggtgtt cagcctcagc
    15001 tgactgcccg gatagaactt ccggggggaa gcatctccga tgtgaaggcg ggccagaaca
    15061 tccccggaac cacttatcac gtggaaaaaa tcacgccgga cagtgtggtg ctggaacagt
    15121 ctggcaccag aaaaaccctg cgaccatgag accggccgcc gtaactggcg gctgtcaaaa
    15181 ataaaaggat gaaagacata ttttccgggc accgctgccc gtaaggaggc tttatgctgg
    15241 atccagcaga cctgccgccg gatgacattc tggattatgt ggccatcgac accgataacg
    15301 ccggacaact gcggatcagg gttgttgagg gaaaaaaaca tctgcgtgcc gtccaggaat
    15361 atctgacccg cctgcgcgca cgctatcagg gacgagtcgg agactttgaa ttcaccacac
    15421 tggatgtgat agcccggatc cgggaagcaa caacaatcgc cggacaggaa aacggcatga
    15481 atcctgtgca gaataaaatg ctcgggtata tccgacatgc cgccaccctt aatgcctccg
    15541 atcttcatat cactccgggc cgggacaaca cagacttcac ctatgtggag gcccgtgtac
    15601 atggggaact ggaaatcctc gacattatcc ggaaagatga gggagtggag cttctggggg
    15661 ccacctactc cgggatgact gacgtcatca agggaacaca atttgatcct ggtgttcccc
    15721 aggacgcccg actggcggaa aagtttctga aacttgcggg gttgtttggc gcacgttact
    15781 cccattatcc ctgcgtgggg ggcctttatg ctgttctgag gctgatcaag gacgacagca
    15841 gccatattcc gaccttcggt atgctgggct atcatccaaa gcaggagcag accatccgcc
    15901 gtattctgca acgaccggaa ggtatcatca ccctgtcagg gccgaccggc tccggaaaat
    15961 ccaccacgct gaggacggcg tctgcggcct atcttgaaca gcacggattc aacaacaccg
    16021 gtggtattct tttgccccgt cgtcgtctct tcaccattga atcaccgccg gaaggacgta
    16081 ttcccggcgc aatccagacc gccgtcatgg acagtgctca gggctgggtg gattccataa
    16141 aatcagccct gcggcttgat cctgacggca ttctgaacgg tgaaatccgc gatcacgatt
    16201 ccgccattac ggcaataaaa gccgccatga caggccatct gatgctgacc accatccatg
    16261 cgaacgaccc gattaacatc ctcgaacgcc ttgagatgga aggtgttcag gccaggatga
    16321 ttgcagaccc gcagttgttc atcggactgc tcagtcagcg tctggttcag ctgatatgtc
    16381 cccactgccg tcatccatgg aatgacgtgg catcccgtcg tactgaggat gaacgacgtc
    16441 tggtggaaac cttctgtaat gctgatgcgg tatatctgag aaaccatgac ggctgcccac
    16501 actgctggcg tggtgtcaca gggcgcacag tgattgcaga agtcatctct cctgatgcca
    16561 gattttttca gttgtaccgg gaaaaaggcc gcatcgaagc caaaacctac tggcacagag
    16621 agctgggcgg gatgacgcgt aatcagcatc tgctgggaaa aatcaacagc ggactggttg
    16681 accctctggc ggcccattac atctctccca ttgatgagga cagctatacg ctgctgcgct
    16741 gatatgaaat ttctggaaaa actcaacagt actgaactgg atctgtctgc cctgaacagc
    16801 tggacagcca gaaaaacctt cggtcttaag cgtgccgagt tctatgagtc cctcggcgga
    16861 atgatacgtg acggaacgcc gtcactgcgc gccctggagt ttatctgtaa tgtcgaaact
    16921 gactttggca cccggaaagg aacatccggc atgtactttc tggccagaga atgtattgcc
    16981 tccctgaaag ccagcggtat gctgtcgggg gccctgccca catgggttcc cccggaagaa
    17041 gcggcactga tcaggaacgg tgaagaacgc ggaaacatcg ccgaagccct gttccaggtc
    17101 gcaaaaaccg tgaaagcacg gcgggaaatg gtgagcagtc tcatcacact ctgtctttat
    17161 ccccttctgc tgctttcact ctgtgtggtg aatatgtaca acgcacatac ccgcatcatt
    17221 cccattgtca gcgcttttgc accggaggag aaatggtcat tccagatgaa agtccttgca
    17281 gccatgtcag agctggtcat cggctacggt ctgtggctca gtggtgccat catcacgctt
    17341 gccatcatca tccgcttctc actcagtcgc tataccggcc cgggacgcag acttcttgac
    17401 agactgccac catggtcgct ctatcagact tttcatggtg tgaattatct gttcagcata
    17461 tcgtcaatgc tgcagataaa tattccgctt tcacaggcgc tgctcagaat tgaaaaaagt
    17521 gcggagcata ataaatggtt aaaggagaga attcaggcaa taagaaaaaa tgtattatcc
    17581 ggtcagaatc tggccaccgc catgcgcaat tctgactatg attttccttc aaaaatatgc
    17641 atcaataaac ttctcttaaa ttctgaaagg gaagacagcg tatctgcaat gaatacctat
    17701 gccggacagt ggctggaaga tgcaaaaaag agcgtcagaa aaagtggatt aattatcacc
    17761 ggtatttcag gaggttttgt attctttttt attcttaaca tgattagcgc gatatacagt
    17821 atcagcgata tgctcaagca ataatacctg ccgtaacact aaaacgccct tcaatatact
    17881 tccattaacg taacaaccat taatacagga gtcatatatg aacttcagag cacgtctgtc
    17941 ttcttatttt tctttaaaaa aagataaaga agaagataaa ggtttaacat tattagaaat
    18001 tgccggcgca atgattatca tcgcaatagt tgtcgcgctc gcgatgagcc agatgtccag
    18061 cacaatggcc tccagtgata accagcgcga actgaaaaac ctgcagacaa ttgcgaccaa
    18121 aatgaagcaa tacaagttcc agggaagata taccggaacg gattatatca aaaccctgac
    18181 ggaaagcggt gcattgcctg ctgatatgat tgcaggaggc aacaaagcca aaaacgcatg
    18241 gggtggtgat gtcacgattg ctgccacagc agacaaatat ggctacatta tcaccagtaa
    18301 taatgttcct aaaacaaact gtgttgagct gattaacagc ctgcgcagtt cgtccatatt
    18361 taccaaaatt atgaacacag ccccgaacat ggtggatccc gttaccgtct gcagtagcga
    18421 taaaaacaat atcaagctgg aaaccaacag ctaatgccct gccacacagc actccctccg
    18481 gggtgctgtt tcccggagca cataatcatg aaatggtttt atctctcatc tcttttctta
    18541 ttctgtctcc cggcgactgc cgccgtaaac agtaacacac tggatagctg ctttattcag
    18601 gcaggaaaac gttactccat cgatcccgga ctactgaaag taattgcccg taaagaaagc
    18661 agcctgaatc cccgggccat caatcataac agaaatgcca gcggaaaaat tatcagcaca
    18721 gattatggcc tgatgcagat taattccaca catattcccg gactgaaaag aatgggaatt
    18781 atccgaagtg aaaatgaact gctcagtaat gtttgcctga atatacagac aggtgcatgg
    18841 atccttgccc ggcattttca gcagtgtggt gtgaactggg agtgtctcgg tagctacaac
    18901 gccggattta ataaaaataa cactcaccgc agaatgaaat atgcccggct gatttatgcc
    18961 gcatatatgc agggacgtta ataacggaga atacgagcca tgttcctcat catcttactc
    19021 gtgatctccg ctacgctgac catacagtta cttgccatca gactggccgg acattatctc
    19081 atgcaggtgc ataccctccg gcgtccacgt cacctctccc tgctctgtgc tgttctggca
    19141 ctcaccgcca ccattacgca catcatacgg gggacagaca ttgccggact ttgctttctg
    19201 ttgctgatgg ccggctgcat gacactgatg acactgactg atgcagcctc ctgccgtctt
    19261 ccccgtccct tcaccctcac attcctgatg ctgggtgtgg cgtttcgttt atggcagggc
    19321 gatgctgcta ttgcgcttgc ctgtgctctg ttctggtttc tcctgttatc tctcctgagg
    19381 cgctacacct cataccgcaa gggagtggag acgctgggac tgggggacgt gtttctgatt
    19441 gcaggtgtgg ccatgtggtc atctgccaac agcactccgt ggatcatcgg tattgccgcc
    19501 gccagcacaa tgatctgtat gtcgctgttc ccgcgcagta atactggggc cgctattccg
    19561 ttcggtccgt ttatctgtgc atcactctac attttcactt tttttcctgg tgccattttt
    19621 cacaccggag ggtaacacca tgcaaaaaga cgacgataaa ggttttgttc tgtttgaaat
    19681 tctggccgga ctgattgtga tcggtatcgc cacaccaatg atttattcag aaattgaaaa
    19741 ctggctgaat gagcagcttt accagagtgc tgcatatcat gcagatgcct acaataccgc
    19801 agccagaaac tatattgccg acaacaatgt ccgtctgcat agcggcagcc ttccggcaaa
    19861 tttcacggct gatgatctga ttcgtcaggg atatctgaaa caggggttta accattctcc
    19921 ctttggtcag agttatatca ccggtatccg ccgtaaccag accaccgggc gtctggaagc
    19981 cctgacctgc tcaactggag ggcagacaat caaagaagaa gggctgcgta gtgtggcggg
    20041 tcagttgccg gggctgggtg gatttatcag caaaaatgga acggcaacag gcgcattcgg
    20101 agcctggaca gataaaccgg gagactacgg acttacctgt tcgacaggtc acatcgccgt
    20161 cgtcatgagc ggtgatgacc tgcaggaaag tgaccgtctc taccgttttc aggtagcagg
    20221 tcgtcctgaa ctgaatcaga tgcataccgc aatcaatatg ggcgggaata acataaacaa
    20281 caccggcaat atcaatggcc agagtgccac actgaaaggt gatattacga gtgaagacgg
    20341 ctggctaatc accagaaaca ataagggatg gatgaatatc acacatggtg gtggattcac
    20401 catgacagac agccagtgga tacgcgcggt taatggcaaa ggcatcacaa ccaccgggga
    20461 aatcaaaggc ggaaaagtca gtggtggtac agtccggtct gacggtcgtc tttcaaccgg
    20521 ggagtattta cagcttgata aaacagctac ggcaggcaca aagtgtgcac cggacggact
    20581 tgtaggccgc accagtacag gggccatact ttcgtgccaa tccggtatgt gggcaggttt
    20641 tgaatcctac cctgtcggaa gccctatacc gtggccatca gccacccctc cacaaggcta
    20701 cctggtgatg aacgggcagt cattttcatg ttcccggtat cctcagcttg cccgtgctta
    20761 tcccgggtgt aagctgccgg atttgcgcgg tgtttttatc cgtggctggg acaacggacg
    20821 tgggctggat ggagatcgga acagacatct cctgagttat caggccgatc aatccggtgt
    20881 ctatcatgag cgtgggggat ggctgaaagg gcatcacagc ggtatgcctt actgggccca
    20941 gggaagcaca acggaaatgc gccccaaaaa cattgccttt aactacatcg tcaaagcaag
    21001 ctgatgcatt taaggcgtat taacttttaa cttacagcaa cgtattttga cccagatcat
    21061 gcttcccgaa tatcccggtt tacctctgta cgctctgaat cctgccgcac catagcctgc
    21121 tttgccgtca cagacggcaa agccgtttga gccactaccc cagttcgcat aaaccggttt
    21181 tctggagtct tcttctgcgt cataacaggc cgaataatca aaaaccagtg catcgctgat
    21241 attattcttc ctgacaccgg attgttgctg cgtgcactgg cccaggctgc tcagcggggt
    21301 gaacgtgtcc ccacacctgg cgagccaccc gcaaacattg atgaacgggt catgatttgt
    21361 ccgttaacaa acagccagtt catgacaaaa gcgaacagta ttttcatatc ctcctcctga
    21421 acaaataatt tttgttcaaa attaacgcta tgagaccctt tatgaaacac ttatcctccc
    21481 gtgcagccac tgcggataat aatcgtatcg ttacaattga agacgtttac acaatcgcat
    21541 tctcgcaacc aattcacacc gaatcagcgt tggggcaaag gtggatgttg atccatactg
    21601 gtgaatatga aagtgccagc gatgcaaaac gcttccttga tacgctcgtc accgggcact
    21661 gtgcttgcat ggtaacacca tcacctgttc tggatcatga cacctggctg cgtatccgcc
    21721 tgctgatggg cgagataagg gatgccaaat aaacattcat ggccccaaca cataaaaata
    21781 acgccgggga ctctggcaga ggtaccattc tttttacata gagccaatat ataaacaact
    21841 tttctatgat aatatcttta tcatgttata aataattgcc ccctcagcag aaagggccca
    21901 aatagaaaaa gcaaaaatcc ttataaaaat attctttaca agcgttccta tatacaaaca
    21961 aactatataa accagccaga acagaatgac tgcaaaagaa acacacacgg cccccataaa
    22021 aataaatgtc atcgaatccc atggtttcac cagtacaacc ggaaccatgg ctgccagaac
    22081 aataagagca gcattaccca acagcgtctc tcgaataata tcccttataa tctccagttt
    22141 ttctttatgc tgtttaattt tcatacgaac ctcgttgatt tcatcccgaa aaggaaaaga
    22201 ttatataacg aaatactttc aatctcctga ctccgtctgc tctgcagcat aatgccagac
    22261 cagcggcatg ctaaatcacg tcctgtccga atccttttca ttcacgctta aaacagagtt
    22321 aaaagcactc ttgaaacaca ctcgatcata acgatcgaaa taactcgttt ccgttcagta
    22381 aacacgctct ttagacttaa catatgaacc gcacggaggt gaaatgaaac ccaacagcac
    22441 aggcattgct gcccgcatga tgctctccat tgacagggcc cggattagcg aagctctgct
    22501 caataaccgc cagttgcagt ccactcccct gcagatccgt tatccgcagg gcgtcagaga
    22561 agcactcgga ataatgagcg agcaactctc cctttctgta tccgacctga cgcgtatcct
    22621 ggtcgaagat gcattaactg aaatgtttct cccggctgaa aacatcgttc accgtcttat
    22681 ctgccggaca gaacacatca tgcaggcaca tgatgtctgc gtaacaacga tgacaacgtt
    22741 actggctccc tggaatatcc gtcctgccgt tttccgtgac ccggacaaac tcacagatta
    22801 tctgacagga gagattctga ctgctctggc tgactggttt tatctcaacc cggaatggct
    22861 gaacggcagg gttcgttatc ctttaagccg tgccggtgag tggccagcca cacaggatgc
    22921 gttctgccag cttatttcag ccagcgataa tgtggatatt attatctggc acagttttcc
    22981 ttttgccggt gttcattcac aggaatattg cggtgtactg ttccgcaaga aaaaagaaat
    23041 taataaagcc atcatttatc ctgtactttc tttatcacct gtaagaatga ataaggaaaa
    23101 agaaaactgg tttcacactg cccgcaattt atatgcaaag acatccgtcc ggaccgtaac
    23161 gcttcgtccg gcacaggcag aatatctgat gacaggaaaa atattacctg ctgagttatt
    23221 cagtataccg ttattaccct ggtaataacg cagagaaaaa accagtaaat ttttaatggc
    23281 acagtcgtgt cataccgttg ctgtacccaa aacacctcaa accaggaaac tgaaatgatg
    23341 atgaatatgc acattacccg aactctcatc gcatctgctg ttttattttc ctttaattca
    23401 acagccgcaa cttcatattt tgaagcccgc aatgatgcaa tggggggaac cggggtagcc
    23461 tcctctcact acggtgtggc acctctggca aacccggccc tgctgacaaa agccggtccg
    23521 gaagatgact tcagtcttct gattccgtcc gttggtgggc aattgtccga tccgggtaat
    23581 atcagtgaca acgcagacaa aatcagtgat gactggaaag cctttgatcg tgccatcgac
    23641 agtcagagca acgtgtcgca gactgctgca cgtctgaaga cgcgacttca ggacttccgt
    23701 cacacgcatg ctgacgcaca ggtgggaaca tctgctgtgg cgactcttcc cggtgacacc
    23761 ctcgctgccg ctctgatgct gaaaagctac ggtacggtca gtgtcgacgg taaagtcagt
    23821 gatgccgacc tgaactatct ggaaagcatt gcaaacagta acggtcagga tgtggataaa
    23881 gacagactga cctctcaggc ttttgcccgt gccgcactgg tcaccgatgt gggtattgct
    23941 ctggctactg aactggaaac ggccggacaa aagtggtctc tgggcttcac accaaaattc
    24001 cagcgtgttg acctgtttaa ctacaacgtg ctggttaaaa attacgacag cagcgctttc
    24061 aaaggcgacc gttaccacaa caccagaaac ggcattaacg ctgatatcgg ggccagcatg
    24121 gacctggatg acaactggac actgggtctg gtcgcacaga acctgctccc ccgcagtatt
    24181 gagacaaaag aagtgaacgg tgtcactgaa actttcagga tccgcccgca ggtgactgcc
    24241 ggtgtctcct ggcacaacga catgttcact acagcactgg atgttgacct gaccccggcc
    24301 agtggcttca catcagacag taaacgtcag tttgctgcca ttggtgctga atttaatgcc
    24361 tggaaatggg cacagttacg tgcaggctac cgccagaatc tggccagcaa tgatggcagt
    24421 gcgttcaccg ccgggctggg tatctcacca tttgatgtcg tccatattga tgtggcgggt
    24481 atagcaggca cagacaacaa ctacggtgcc gttgcacaac tgcagttcac attctgatca
    24541 tctctgcccc ggtactgacc ggggtatcca gacctgcatc ctcgcgtttt ccgaccatca
    24601 aacgacacag ccagacctgt agtgctgcct ctgatctggc tgttatctga catatcattt
    24661 tcgcatgagg aaatgcatgt ttaacaaaaa taaaacgcat atcgaaaaca aagcctttat
    24721 ggtggaaccg ttccgggatc cgacagctaa cgaatgtaat gtcgtgatag caaacgatat
    24781 ttgtatccgg gtagcactgt caccctctga ggtgctccag ccattcagca aaaacacgaa
    24841 ccagtaaatc ctccccacaa cgctttcttc tttcactgct caggaaaaat acaccatgaa
    24901 ccgtatgata cctgcgggat gtctcgccgt actctgcctg aacggctgcc atctcccccc
    24961 ggataataca gcgccgacca gaaagtatgg tgctgcctct gatgtcgccg tcgcccggca
    25021 tgcgcagtat cagctatggg ggcagggaat ttactctgca gcccttccgg agcgtcagtc
    25081 cggcggcagt ctgaaggctc acagcgaccg tgtttccttt gactgggaag gcgatgccct
    25141 ggaattgctc aacgaactgg cccgtctgcg cggccagcag ttcagctaca gcggcgtccg
    25201 tctcccctta cccgtctcac tgcacgtccg cgacatgacc tttgctgatg tccttcgtct
    25261 cactgaagcc cagactgcct ggcgggcaac actctcgcag tcagccgggc gtttacatct
    25321 gtcttttatg acgccggaga aaaaatgaaa acacacctgt gtctcctgct gtctgctctc
    25381 ctgcttacag gctgtgccgg acagcatcac cgttcttctg ccgtcatgac agaaaacgca
    25441 ccgcccccgg atatccgtgc atggctgacg ccctccggca accaggatga tggtatttca
    25501 gatacccgtc gtcagatgct cacggatgcc ggcagaacgc tgggattccg tggtggaaaa
    25561 gcacagcgct catgggaact gattcaggca ctgaatgccc gggaaagtac cctgaatgcc
    25621 ctgtatgatt tccggcctct catcagcaag gaaggctggc tgcctcctgt tatcgatgaa
    25681 gcgcaggatg ttgcacacat cagaccggac cagataagaa cagcctcccg tgtctggact
    25741 atcctccgcc cggaacgctt tgtcagtaat ccacctggat ggcgtgactg gctgctgaaa
    25801 ggactctcaa ccaccgccac accaggcact gaaggccggg ttgtgccgga agaccgggca
    25861 caacgcagac tatgggaaaa cgccttacgt cagggatggc aggaagggcg cctcaacgca
    25921 gatctcacac tggaagctaa ccagaagcgc ctgacccgtg attaccgggg catgatgctt
    25981 tacgcccttc tgtggcgtca gggcatgatc acacgtccag atgtcacaga gcagatgcag
    26041 acggttaccg gcaacgggca aaaactcatc actggcgatc gcgtccgccg cctgaaaaca
    26101 catgctgaat ttacgctgca gaaatcgcgc tggcgtcctc ttgtcagcac cgaaggagag
    26161 aaaaatgata tcaccagatg acttcggtac cttccccttc tcccgtttta cggcagaaga
    26221 gtttcgccat ttttttgcct ggtgtgcccg ccaccgcgtc agtgatattg atctgaccgg
    26281 cggttcaccg gtatccgtca gtcgctttgg acggcgggtt cgctcatccg taaatcccct
    26341 tccgtcctcc atcatgatgg cactgagtga tgagctgttt gggcgggagg ttcttccccg
    26401 ggttctgtca ggtcatcccg tggatcgtac cgttcaggtc agcggagatg cctccggtcg
    26461 ctatggcctg caacgaggtc agcgtgttcg cctgcgctgc cacctgatac agggaacatc
    26521 ggcaacagaa gaaaaagcca tttccgtcac catgcgggtg atcccgtcag atattccgga
    26581 catcctcact atgaatattg aaccggatct gctggatgct atgctcagaa aaaccggcct
    26641 gggcttcatc tgcggagaaa ccggctccgg gaaatccaca ctggcggcgg cactgtatcg
    26701 ccacattctg aaaaatttcc ccgatcgcaa aatagtgacc tatgaagatc cggtggaata
    26761 cattctgggc ggagagaatg accttctgcc tccccatcag gccgaaatcg ggcgcgatgt
    26821 ggaaagtttt gctgcaggac ttcgctctgc cgttcgccgt aatccggaca tcattggtat
    26881 cggtgaaatc agggattatg aaacagcaga tgccgcagtc caggcgggta ataccggaca
    26941 cttttgtatc ggcaccatgc acacaaaatc acccggagaa acctttgccc gcctgctggg
    27001 attgtttcct cctgaaatca gggattcgat ggctgcagcc acgctttccc tcgtacagtt
    27061 cattctggtg caggtactgg tcagaacaaa tgacggcggc aggcaggctg tccgggaata
    27121 catcgtcatg actgacgaac tgcgcaatac gctctccgga caatcccatg caacatgggg
    27181 acattacatt gacgaaatca ttcgtcagga aaaacggcgg ataagggatc aggttctgac
    27241 catgtatcag aacgaccgca ttgagaaatc agaagcagcg ctcttcatcc cggccggaga
    27301 gttcccccga tgaaacgcga aggcttctgg cgggatgtaa caccaccgct caacatttat
    27361 ggtgtccccc tgccgtttac catgatttac ctgctgttgt ttccgtatcc gtcaaaaacg
    27421 acattctggg tgtgcagcgg cattctgctg ttttttatgc tactgggata ttccggctgg
    27481 acagtccgca ccctctgtat acgtctgtac agcctgcttc agggtaagca ggcatcggga
    27541 cgtccctggt ggtaccgaca ccgcacagaa ttaccccgtg aatggcacgg accagaatag
    27601 ccctcttttg tccatcatcc cggcgtatca caacgccctc atatttaatc cccggagaac
    27661 gacatgaagt ccagatatcg tacctggctt gctgtcccgc ctgaggagac agaagccgta
    27721 aaaaatgcag tccccccgtt aaatgggtaa gcgtcaacgg agcaccgtat tgacgcttat
    27781 ttattggtga gtactacgtt ccatggcagg agttcgtcaa ctcggttgga aggccattcc
    27841 ggcagtacgc tcaggatatg gcgcagatac gcttccggat cgataccgtt cagacggcag
    27901 gtgccgatca gcccgtacaa cagtgctcca cgctcgccgc cgtgatcgct gccaaagaac
    27961 ataaagtttt tctttccgag acagactgca cgaagcgctc tttccgcagc attattatcc
    28021 gcctccgcca gaccgtcatc actgtaataa cagagggcat cccactgatt cagtacatag
    28081 ctgaacgctt cgcccagtct ggattttttc gacagcgtac cattcttctc caccatccat
    28141 tcatgcagcg acgtcagtaa cactttgctt cgctgctgcc tgacggcaag acgctctgac
    28201 tccggtaatc cccgtatttc atcctcgatg gcgtacagtt cactgattcg cttcagggct
    28261 tcttctgccg tcgcactttt gctgctgatg tatacatcgt ggatttttcg ccgggcatgg
    28321 gcccagcacg caacttctgt cagtgcacca ccttcacgtt ctgcactgaa caacctgtcg
    28381 taacctgtga acgcatccgc ctgcaggata ccccggaagg ggcggaggtg ttgctccggg
    28441 tgtttcccct gccggttcgg cgagtacgcg aaccagaccg ctggaggaga tgacgaaccc
    28501 acattgcgat catcccggac atacgtccag atacgccctg ttttcgcctt tttctgaccc
    28561 ggtgccagta cctttaccgg tgtgtcatca gtgtgaacct tgcgggtgtt cattacataa
    28621 cggtacaggg catcattcac cggtgtcatt aactggcagc acgcgtcaac ccagttggag
    28681 agtaaggccc ggctcagttc gacaccctgg cgggcaaaga tttcactctg acgatacagt
    28741 ggcagatgtt cgcagtattt tcccgttaac acgcgggcaa gtaatccggg gcccgcgata
    28801 ccacgctcta tcgggcggga cggcgccggt gcttcaacaa tacagtcaca ttttgtacag
    28861 gcttttttta cccgttctgt gcggatcact ttcagggcac tgctcaccag ttccagctgt
    28921 tcagcgctga cttcccccag ataatccagc tcaccgccac actccgggca acagctttct
    28981 tctggctcca ggcggtgtat ttcacgggga aggtgtgccg gtaacggacg acgatggcgc
    29041 gactgtcgca actggcgggg aacctgagga tcgtcttccc gcccactgta acgatcgctg
    29101 tcctgttcac gttgtttcag cagagcctca gccagttcaa cttcacgacg cagtttttca
    29161 gaacgggtac cgaacagcat ccggcgcagt ttttctatct gagcccgcag atgttctatt
    29221 tcccgttcat cttcttcgat cttttcttcg gcacgtgtca gtgcagagcg caggaaggct
    29281 tccgtctctt caaccagact cagttgctgg tctttctgac ggagggcttc agcctgctca
    29341 gagagcaacc tttccagctc tgcgatgcga atgaggtatt tctgactcat gaccgttttt
    29401 ataatgcggt caggagtttt ttacaacatt gtcagtgagt tacggctgga tgtttttggc
    29461 tgacgccagt ccagcttatc gaggagcatt gccagttgcg agcgggtaat ggataccttg
    29521 ccgtcacgta ccgcaggcca gataaactgg ccttcctcca ggcgtttggt gaacaggcac
    29581 agaccatcag catcagccca aagaattttg acggtgtcac cccgtcggcc acggaagata
    29641 aacaggtgac cggagaaggg attatcattc agcacatgtt gtacctgttc tcccagtccg
    29701 ttgaaggatt tacgcatatc ggtaacgccg gcaacgagcc agatacgggt acctgatggg
    29761 agtgagatca tcttcccctc ccggtcagtt cacggatcaa cactgtgagc agctctggcg
    29821 atggattttc cagcgtcatg ttaccgtgac ggaattccac cttgcaggaa ctggcactga
    29881 ctctggtctg agtggaagtg gataaagacg gcgcaatggc cgccacaggt tctttctgct
    29941 catccggcgt tatttctaca ggtaataatt caacgccagt gtcagaagag gtcgttaccg
    30001 gaagacgccg cgaaacacgc ccttcgttct gccagagcct gagccatttg aaaataacat
    30061 tatcattgac gccattttca cgtgcaatct gtgcaacaca agctccaggt tgtgatgcca
    30121 gttccaccat acgaagtttg aattcattcg aatagttttt acgaggttct tttcgccagt
    30181 cctgtaattc catacttaga tgtccgtcta tatcagatgg gcgtctaagt taccaattct
    30241 cgtctgatgg ctacatacgg cggtcagttt acgcttactt aaatggccgc aaagcggtag
    30301 catgggatcc agagaaaaaa ctctggtatg ccgaggcggg taccgacctt tcactgctga
    30361 agcgctggtt accccgtcca caggaaatgt cgatggatgc cggcgatccg gtcacagaat
    30421 ttcgtcaggt tctggaaaac gccgggctgg ttcttgagaa agatccagaa atggacggag
    30481 aaatacacag agtgcccaca cgggatgaca gaagaggctc ccagagtggc gcatacaagg
    30541 cctatcttga tggccgcccc tccggctggt accgcgatta ccggagcgct gataacagtc
    30601 ccacaacctg gacattctcc cgcgcagaaa atatcgatcc tctcgccaga ctgcatctga
    30661 aggcatccgc tcagcaaaat cgcgatgaga aagaacgtaa actacagcaa cagtacaaca
    30721 aacaggccgg ctatgcccgc tcctatctta acgggttgcc ccaggcaaca gcacatgaat
    30781 acctgacacg taagggcatc cgggccgcgc ctggcgtgcg gctgaacaat aaaaatgagc
    30841 tggttattcc cttcagcaac ggccgggggg aaatacgttc gtaccagcgt attccggtca
    30901 ccggcgggaa ggatgcccgc attctgaaag gctctgagaa aagcggcaac tggttcgcct
    30961 tcggaacccc ggaaaatggc cgtcccctgt tatttgcgga aggttacgct actgccgcct
    31021 ccctgcacga agccaccggc catccggttc tgatgacaat tgatgccgac aatatgatta
    31081 atgtggcaaa aaatgcccgc aaaatctgga ctgacagccc ctttgtcttc tgtgccgaca
    31141 acgaccatca acggaaaatt aataaaggtg tttcaagtgc aaaaaaagct gccgcaagca
    31201 cgcatggcga agtcattatt ccggccttta cggaggcgga aaaagcacag ggactgaccg
    31261 atttcaatga cctggacgcc agccggggac gagacgcctt tcagcgagcc atgaacgcac
    31321 agttagaata tatcggtatc cccacgccca acaatgacac tactgaccgc cgtgaagcta
    31381 tcgtgatcgg caacctgata ttcacaccgg taaaagatga gaagccacaa gtgtcccctg
    31441 aaaacagaca gagcacagcc cctgaaccgg cagtgtcgga acagaatcat ccttatgaca
    31501 tccctgcaga agagacagac aatcaggcat atgaagatga ctatgcagca tatgcatccc
    31561 tgcagtcggt aactgcaggg acagcctccc cgaatgaacc gcaggtgaat gctgtttctg
    31621 ccattgacag caacgcaaag aatacctctg ccgccaccgc tggcgaccga aatgtattag
    31681 cacaagaacc caatgctgac gccacggtta cgaccacggc tcacgacacc ggcacagcag
    31741 aaacagattc accttctgaa cctgcagaga ttcagccagt gcagcaggcc agtcctgaac
    31801 catcggctaa agataaacca caggagaacg tagtgtctgc tactgataac gacgcagaga
    31861 acacctcttc tgccaccact accctccgga gtaacgacac accggaaaca tccgtctccg
    31921 acgcaaccgg gacacagcac accgccacaa cgcaacctga agaaccacag caggtctcca
    31981 tgggcagcgc tgacgactgg agcgcatttg cggaagacct caatgctgat gctccgggga
    32041 atgccactgt tcacagcacc gacaacgcgg agacaaagac actttctgaa cctgccggga
    32101 tgcagcctgt tcaacaggca acccctgaac caacggaaga aaataaacct caggaagagg
    32161 gcgttatgtc tgccactgat acaggtgaag agagtgccgc tgccgctatc gataacgacc
    32221 tgaacacagt tgcggaagac ctcaatgctg atgttactgt gatgaccaca gctcaccaca
    32281 ccgatacagc agaaacagcc tcacctgctg aacctgcaga aattcagccc gtacagcagg
    32341 caacccctga accaacggca gaagataaac cgaaggaaga gcgtcctgtg tctgccactg
    32401 agaccagcac agcgaatgaa acagcccgcc ctgaaacacc atcgcttttc agccgtctgc
    32461 gccgttttct gaccggtgga aaatcacgta ccgccgaacc tcccgttatt caggagaaca
    32521 agcttacgga ggccgaaact gcgacagagg ccgcagcacc accgtcacct cctgaaacca
    32581 gcccggaaga cacatcgtca gcggaaccgg acacgattat ctttgcaccc cgccgtcccg
    32641 acagtccgca gcggcacaac ctggacgaga tccttaaaaa tcttacacac aaagagtttc
    32701 ccgacaggac tgcgctctac agcgttgacg gtgaacccgc cttcctggac agactctact
    32761 gtctcgaaat ggtggatggc gccagcgcgg atgacaaaaa ggtcctggct gcccttgctg
    32821 tggccaccaa cttctatggc ggcgttatcg agctgaccgg cagtgatgcc tttaaacaaa
    32881 aagccatgca gctgattgtc gaatatgaca tcaaggttcg catgaaactc ccggcccagc
    32941 gggcagcact ggaaaaactc cgcaaggaaa aagagatggc gcatgatgcc attgtcaccc
    33001 acagacccac gccggaactc aaccgccagt caccggaaga gacaacaatg gcccaaccgg
    33061 ctccccctgt gtcaccgaag gcagaggctg aacaaacagc aggcagtacg gctgaaacac
    33121 cgcatccgga agcgccggtc acggcaaaag ctgaagcccc ccgggccgac agaccggaac
    33181 aacccgccgc cccgtcgtct gcgccagaaa aagcccctgt cagtgcacag ccgcagcaac
    33241 ccgacacacc ggaggaagag ccgcccggca agctcagacc cggtgaaagt gtgactgccg
    33301 tcctgacaaa ctacggagaa aaagagtatg cgcccggaaa agggcgctgc ttttttgtgg
    33361 agctcaccaa ccgcagtggc aaacgggaat actggggaaa cggcctggaa gaactggtca
    33421 aaaaccatca gaagggtgac cccgtcacac tgacactgca ggcgagagaa acattccggc
    33481 tgtccggtga aacgcgggat cgtatccgta atatctgggc cataaccccg gtcagtaacg
    33541 gcattaccgt taataatgac caacctcatg cgggtcagca tatcctggaa tttccgatga
    33601 aaacattcag tcaggtaatg tcacaaatgc aacaacactg gccacagata atgcaggaag
    33661 ttcgttcccc caaaaatcct cccaataatc tgtacattgg tgaagacagg caacctgcat
    33721 cagcacctca ggatcaatca atgcgactcc ccctcgctgg taatgccccc aatgaactga
    33781 taccagccat cgcctctgcc gataaggaca atcgtcagtt gaatcttctg ctggtccaca
    33841 gcgcagatga tcatctgcag ggcgtggtcc gcctgaacgg caccctttac ccggcactgg
    33901 ccaccccgtc agctgacaac cgtcagctgg tcatcaatgc cctgacggat aacggtctgc
    33961 agtttgccgg ttacggtgaa gctgttaacc atgatgagaa tactcatcaa cgtccgtccc
    34021 cacaaataat gcagtttcac ctgaagcaac aggactcccc gttgtttgcc gccatccaca
    34081 aaccggaaga gcaaccggac aagcttttcc gctctctggg ttttgagcag acctggaaag
    34141 agtggtctga cagccaaaag gcggaggaca ggcaggaaaa aactctgcaa caggcacaat
    34201 cccacagccc cggcctgtga gccggtaagg acgaccatga acatgaaaaa aacgctgctg
    34261 gtcatactga ccagcatctc gctggcttct ccggctgccg tagcagcaac ctgtcgggca
    34321 ggtgctgccg cagaagccgg cagcaaagcc gcctgggagc gggcgcgcac agccagccag
    34381 gcatggtcac agcgagaaaa cgacgtcgcc tcctccctgc agtcctgcct gtcccgcatt
    34441 aaggacgtca gcatcactct tccgcagttc cccagcctgg acaaactcct ccgcgacctg
    34501 gaaaaccagg tctgtgatgc cgtcgtggat cgggtgaatg aacatctgcc ggggaatatc
    34561 gacccctgga aagactataa cctctgaaca ggagccccat gagcgaagca caacccacct
    34621 cccctgtctc accggaagcg gataacagcg gagatattta taaccccgtg ctcagaacca
    34681 tcgcacagtc agaacaacgg gccgcactgg ttcccggact ggtcagggcc ttgctgtgta
    34741 gcagcgcagc tctcgtcatc agtctgaccg gcaatatcat acagtactgg cataacagca
    34801 acgtaacgcg tgaatacttt gccaccgaca atggccggct ggtccgcctg gcccccacca
    34861 gtcagccagc ctggagtcag agcgacgtca tgacgtttgg cagcatgacg ctgagcgaag
    34921 cttttaatct ggattttgtc cactacagga aacaaatcac gacactggcc ccccgctttt
    34981 ccgaagccgg ctttgccggc tatgtgaatg ctcttcaggc ctccaacatt ctggacacca
    35041 ttaaaaagga gaaatttaac ctcacagcca ccatcggggc cggcacgctg gtgaatcagg
    35101 gccagatgga aaacggcatc tggttctgga aattccagta tccggtcagg atgcgtctgg
    35161 tcggtcagac cagcacccgc cccgagcagg ccttcacctt tgaaatcact gtccggcgcg
    35221 ttgacccacg aatcaaaccc ggcggcatgg aaatcagcca gatggtttcc cgtaacgccg
    35281 catcaggaac ctgagactca tatcatgcaa atacatcatc ttcttggcgc actgcttatt
    35341 gtgtgcggca ctgtcacggc tgcagataac ccgggctggc agaacgcacg aaccccacag
    35401 gctggcggaa agacaggaca gactcccggg agcgcccccg caggcggccc cccgccagcc
    35461 acacaactca caggccccct gccgccacct gcctctcctc ttgttcagga agccgcatca
    35521 ctggattccc ccctgaatgc aggtgaaatt cgggaactgc ggggacgaat ggccgataac
    35581 gaacgggcca tcagcgcacc tgtcaccagt gtggtgcccc gtatcagctc cctcacggta
    35641 aatctgtcac ccggggccag tctgccgctt gtccgaaccg cactgcataa cgtcagcgtg
    35701 gtaacgttca cggacatcaa cggctccccg tggcctcagt cggatccgcc ttataacgcg
    35761 gcacccggtc actttgatgt gcagttcaac ggcaatatgg tctccgtcac tcccctgcgc
    35821 gcctgggctg cagggaatgt aacggtctac ctcaaaggac tgtctgtacc ggtcattctg
    35881 aatatcacca gtggtgaaac cgacaccaat accctgagtc aggaaatcga cagccgtctg
    35941 gatctgcgca ttccccgtca gggacctgcc agtcctgtgg tagccgtacc atcagagaag
    36001 attgccctgc atgatgccac tctgcaggcc tttctggacg gtgttcctcc cgatgatccc
    36061 accgtgcgtc ggctgaaatt ctccggaaat gttccggaca ccaccatctg gcagcacggc
    36121 gatgatctgc tcgtgcgatc gcgggccatg ctgcgggatg aatttgaaca gacgctggca
    36181 tctgctgacg gtacgcgtct gtggaaactg ccggtgaccc ccttactcac cttctccgtg
    36241 aatggtcagt ccattcacgt cacccctgaa ctggagtaaa tcatgagcgc agaacaggac
    36301 acagccgcaa agtccggcaa aaaactgtca gccctgctgg ttctcggggg cgtgattatc
    36361 atcgggggcg gatacctgct gctcacctgg ctttcccccg aaaacagcgc atcgctctcg
    36421 tcagtcaata tcaacagcac gacaacaggc agcgggcgga ccgtggtgga gtcacagcac
    36481 taccgggatt tactgcgtgc tgacaatgag cgcggggccg cagaagcatt acgtaataac
    36541 cagacgttca tcgccagtct gcccagggga ctggatactg atcacggaaa gaatcaaaga
    36601 agggatactc agcctgctga ccaggccgag gggtctggtc aaccatcaac gccccctcag
    36661 gcagatcaaa cagcccggga aaaacagcgg gaagcgttac aaaaactgct tgcccgcatc
    36721 aacacacagc atcctgacgc ccgccccccg gtagtggcgg ctgcaatgtg gggcaccact
    36781 ggtgctcccg ggataagctc acatgcctca ccacccggta ccggcacatc accacaacca
    36841 cgtctgcagg aggccagtct ggcaaccccg gcagctcgta cagggataca gctgataccg
    36901 gcactgaccc gcgtacctgc cttcattgat acggaagtgg actctgacaa tgctgtctcc
    36961 cgcgtggtgg cctccgtacc tgccggccca tgggccgggg ccgtcctcta cagccaggac
    37021 gccagactcg ccggcaaggg aatggatatc cactttgacc ggatgatgtg gcggggcatg
    37081 tttctgaaag tcaatgcata tgccctgaat gaacataccg gcatgtcatc cgtggccagc
    37141 gacgtgaacc accgctggtt taagcatatc gttctgccgt ctgtgctcgg tggggtgggc
    37201 agtgtcggga gtctttacaa agacgccaac acacaggtca ttcagggaaa ctacggcagc
    37261 attaccggca gggtgggaat gcctgatggc gaggcgctgg ctggcgtcat tgcaggcggc
    37321 atggccgaac ggggcagtca gttgctgacc cgccaggcgg agagcgaacc ttaccggcag
    37381 gtgacagtgt cacgcggaga ggttgtctcc attctgtttg ttgagccggt catgagcaac
    37441 gatatcagtc ccccctcctc cggcactgcc ccttctgtgc caccgccctc cggacgcccg
    37501 ctcacccagg aagaagcaga gcgtcaggcg tctgacagga tccaggcagc tgtcatacgt
    37561 aaacagaacg aaatgcggca acgttacgga atacaacagg aaagtcatcc atgaaaaacg
    37621 aacatgaaga tcccggcagt tttggcgaaa caacgacacc ggcatccggc aaatccttcc
    37681 tgaaacgcca gtatatggga tacagcctgc cagtctgggc cggctgtgtc ctcatcatcc
    37741 tggtcgcagt ctggtacaag tttctgcgga caccctcaga gccccctgca gatgcaggct
    37801 tcgctgtcac accgcccgca gccgaaagct gggcagagcc catgcccatg aaggagaccg
    37861 ggagcggaaa caccatgtcc gcgcctcctg tcaccccaca ggcagacatg cagcagcttg
    37921 cccgtgacct gaaagcagag ctggacaggc gggatgagga aatccgacgt accctgacca
    37981 tgctgaaaga gagcatcaca acccttggcg acaccatacg gaaagatgag gcctgggcac
    38041 aggaaacccg tcagttgctt gatgaaaccc gtaagcaggt gcatgccctg cagatgcagg
    38101 gaccactgca aatcccgacc gggaataaca gacagcctgc gaaaaccccg ccttcccgcc
    38161 tgcagggcat gaaaatcatg tcggtggaaa aaggaatggc ctggatccgc tggcagggta
    38221 gcgcctgggc agtacgggaa ggtgacaggc tggggaatat caccatcacc cggattgaac
    38281 cgtccagccg ctcagtcatc acctctgccg gtacattacg atgatgagca ttgatgccct
    38341 gacagccata gagaactttg ccagcggtat attcagtgca ggcatggagt ttctcttcac
    38401 ctggggagag ctgctgggga ttatcggcct gattggtcat ctgatgcgcg cccgggcaga
    38461 agggcgtcac agtatgggac cgggaaaatt tatcgccggt atcgtcatct gcggcatgct
    38521 ggtcgccctg ccatccttca tcaatgccgg gggtacccag atgggattca gggctgattc
    38581 cttcggccct atcgcttatg tacaaccaac cacctttggt gccgctgccg gtgccgccaa
    38641 tgccatgctc tcactggtaa aactggccgg agtggggttt gccatgaacg gcatttccat
    38701 ctggcgtaaa tccctgctgg acgggcatac cgccctgagc agcagtgagt ccatcagcaa
    38761 aggaaacgtc aagtttgttg ccggcgttct gctggttttt atcgatcgcg tcatcgatgc
    38821 ctctctggcg agtcttggtc tcgccttctg atccacattc attttcagta aatcagtctg
    38881 ttagttaagg agacactata tgtcacgatt ccgtgccatg acagcacgtc tgtgcgctgc
    38941 atttatttat gttcagttat acctggtggc ctgccgtgac cggctgatac gcctgttact
    39001 tctgcttccc gtgatactgc tgccccacac agcgatggca aacaccctct tcgatatgat
    39061 caaaaccgtt ggtgatggtg caaaaggctc cagcagttca tttgtggcta tcggtcaggt
    39121 tattggcctg gtgctcttta tcggcggcct gcttggcctc aaacaaatcg gtaaaaaacc
    39181 cggagcaggg ctggcctgcc tgctgtcaat tctggtcggc ctctgccttg ccgtcgtacc
    39241 ggaactgatg ggcagcggac agaaaacgct cggtctttct ccggtttcaa tcagttaatc
    39301 cgcaaaggca ccgggaaacc ggtgccgtct tcacacaatg aagaggacca gaccatgtcc
    39361 tgtgagctgc cccctttcac cctgctcaga acagagcacg gcaacgtgat tgcccgcgac
    39421 aaccgggata atcaggagtt tgtgtttgca gagtgttatt ccaccgccat cgcagcactg
    39481 attctggctg ccgtcaccct gtatacagag agaaaaacgt atgaacaaaa caccgccact
    39541 catcgccccg gtccgggaac gctggctttt cccgctcccg caaccggaca cggtattcag
    39601 cggtgaatcc ctgctgccac cacccgtgct ctcaacaccc ggacgctgtc cctgctgtcg
    39661 caggaccgtc actcaccgct tcattctgga ggattcatgg ccattacagc agatggctga
    39721 cacctgccgg gatacggtcg tcctgctgga aaaaaacctc acccgggtga tgcgcctgaa
    39781 aaaacatccg gtaccggaaa acgctgacga aaaaaagaaa cacacccgaa ccctgcagga
    39841 tgcggagcgg tccctggcac aggcccgcct gtcggccagg cgactggccc tgcgtcatgt
    39901 ggaaaagtca caaatcgtca caacagatgc actcagcgaa aatgaaagtg aactgctgca
    39961 gcctgaaggc ccgccctttc atctctgtgc cttctgccac gcctggcact gcctgaacgg
    40021 ctacgctgcc gcacaggggg tgatggtctg gctccctgat ttgcatccgg cctcggtagt
    40081 tgctctcaat gcacgggcac tgaaggaaat tttcagcgat gagcgtaagc gaatcagaca
    40141 gggacgggcc gtactgaatg ccctggtaca gaaccgcctg gccgttgagg agaaatttcg
    40201 cacctggcgt ccggcggatt ttgctgatgc actgcgccgc tggccgccgg cacagcgtca
    40261 aatgttgcgg gagaaaatgg acggaatggc gcttatcctg ctgccggatt cgtttcctga
    40321 caaaaagtac gtcatgtgac acacaccgct gccggtgacg ttctgccggc cccaactgac
    40381 agaggaacaa aaaatggata ccctcactgc cggatatcag ttttccgcag atgcattccg
    40441 gcactttatt atccggacgc tgacggacga tattccgcca gatggagttg aaaaagagat
    40501 tgcccggttc attctccgcc acccggcatt taacggcggc tggctgctct ttctggctgc
    40561 agaaattcat gaaagatccc gtacccataa cagcatcacc gtcacagtga gcgtcgataa
    40621 ctatctctca tggatggata ttgccgtgtc cggtctcccg gaattaccgg atatgccctt
    40681 ctgtgatttt ttcattcgcc gccagcagtt tatttatccg ggtgtacccc cgacaaaatc
    40741 aggagaagaa tgatgaaaat tcaccgcata tccccggaga cgcttataac actcatcctc
    40801 gcacacctgg ccggaaaagc tgacagtacc gccaaagaag agcaccggct actccgtcgg
    40861 ttccttcgga atgatgacgg caggctggcc ggcatactgc tgaatattgc cggaatactt
    40921 cagttcaaca gggaattaag cgcccggcat aattatccgg ccacacccct gactgaattt
    40981 tcattaagga aacggggaaa acaacttcat ctctgtctct gcagtctccg tttcttttat
    41041 attcctccgg tatttataca aaacaaaaga agaaaatcta ttgttgtaca tcttaataag
    41101 atcacttata aacaaaccca ttcaatacgc tgacgaagtg attcatactg attatactca
    41161 tctttgccta tatgtttttt aagcgtatta acaatatcat attgaggatg tcctgaattg
    41221 tcagtataca gtgttgttgc aggaaggact gcataatctg atatttcaca acctgtatac
    41281 agttctttgt cagttcggca ggaatggcaa agagaaagac atgtatacag gtctgtgttt
    41341 tctgcctcct caggagataa ccacgacaca tattgcaaat catctgtatc aggataacaa
    41401 ttttcttcct gaacctgaac tctgaaaata tagaaaaata attcatcttt tgaaaggcat
    41461 gctattttat tcccccaatg caccgcatta tcaagttgat ccgataaata aatccatcct
    41521 ggtgtcgtct ctaacgacca gcatataaca gaaggagcat tagttcttat tttcccatct
    41581 tttattattt gcaggcctct gctcattgtt gtcgcatgaa aaagatttaa tattttattt
    41641 tcagccttca ctctatttcc tcatttccca agaaacatat taaaaacgga aaacattaca
    41701 cataacctgg aggacaaatc atgactttaa atttccctgc cggggaaact acactattct
    41761 cagcaaaaaa catcctgaca gcaatactca ctctgcacaa cttacacgca tagcgttccc
    41821 atttgaatac agttgcagcc cgcgatttat tttgtcttcc ggagccacct ctgccatgaa
    41881 tatcaaccgc attatttttg ccattgaaga ctgtatcagt accctgtcac ggtattccgt
    41941 ttccgcattc ttccccgatt actgtgatct gcacaccgtc atcggtctgg accagcagga
    42001 ccgcgaacgc cgcccctggc tgaaagcccc ctacattgcc accacaaaac aggggtatta
    42061 cctttccgtc tttgaaatca gcggtgccct gaaagaaatg gatgaaaacc cggacctgac
    42121 agcccccggc acactggaat cactgattac agcgctggca gagcgaatga acactgccta
    42181 taaaaacatc ggtcacaaaa tcagttttgt tttcgaacgc gatccggagc gtggaaggaa
    42241 tgaaatcgaa gccatgctct taccccagcg aaaatccatc gcacgcacgg ggatcctgct
    42301 tcaggacgtt ctggatgaaa aagtcaccac gctcaccccc tggcttgtcc gggaacgctg
    42361 ctggctgacc gtctggagct ctgaagaact gctgagccgg accgatcgca aggaccacca
    42421 gacccgtatc aggaaactcg ctgaacacgc tccgccagcc cgatttgcgc aggatccatg
    42481 gcgctggaca ctttccgcac tcaaaatacg gcatgacgcc tttctggaca cgctggagca
    42541 ggccctgaca catgattctg atggtctgct ggttcgcctg atggatattc atgaagtggg
    42601 acgggaaatc cgccgtcaac tggagcgcaa cagcacacct gcagtatggc agcctcatct
    42661 gcctgaagat gcccgtcctg ccggctggcg acagggcgag gatacctctg tattccacgc
    42721 accgtcgctg aatctgcagt tattcaccac acagcctgaa acacacggca gcctgataaa
    42781 ggccggtgac ctctggcacg gtatggtcgc cattaccctg cccccccaga atctgcggac
    42841 attcaaccag ctggtcaggg atgtgccccg cgcaatcccc tggcgtatcc ggatggatct
    42901 gatgcccggc ggcatgaagg cgcttggggt caagaaaacc ctgctgacct acagcagctt
    42961 tatttcccct ctccggccca tgtatgactc ggtgatgatg ctgaatgaca tcaacaaaca
    43021 tgatccggtc tgtatcatga ccattgttgc caccacatgg ggaaacagcc gggaagtctg
    43081 tacccgcaac caggcgctcc tgaaatctgc gctggaaggc tggggagtat gcggcaccac
    43141 caccaccttc ggtgatccga gacgtgcctg ggtgaacacg gttctggcgg cctcccacgg
    43201 ttctggtcca gtcccccttt acccgcccct ttcacacgcc ctttccctgt ttcccctgaa
    43261 cagagccggc tctgtctggc ggggacaggg aaatctgatg atgcacaccg aggacgggtc
    43321 tgcatgggag gtcgcgctgg catcgtcaca acagaacaag cacacggagc tgacgcccgg
    43381 cgcacccggt ctgggcaaat cggtactcat caacgcactg tcggagatcc agatagcctc
    43441 agcccagaag aacctgcctt tcattgccta tatcgacaag ggattcagtg cacagggact
    43501 ggtacagctt atccgtgaca gcctgccgga gcagagaaag gacgaagccg tggggatcat
    43561 tctgagcaat gatccggaac acacccgtaa cctgtttgat gtgatgtacg gtgctaaaaa
    43621 acccgccacc ccggaaaaga acttcatggt atctgtactc tgcgcactgt gcgtggatac
    43681 cggcaccggt cagccctgta atcccggtga cacccggcaa atcataagca acctggtgga
    43741 tctggccttc agggagtatg gcgaaaacaa ccccagactg taccgtgcag gaacagagcc
    43801 ccttgtggat ctcgccctgg aagagagtgg catcgcagaa caacatgacg ctggatggtg
    43861 gaatgctgcc acctggtttg aaatcaggga catgctgcat atggctggca atatccccgc
    43921 agcccagcgg gcacactacc aggccatgcc cctgctggct gaaatgtccg ccctgctggg
    43981 acagccatcc atccgggatg tgttcggcac agttcagcgc gataactcag cagaattgtt
    44041 actggactac atccgccgtg ccctcgatca gggacacagt gactacccga tgatgtccgg
    44101 ctgtacccgg tttatgctca gccccgacac ccgcgtcgtg gccgttgatc tgaacaatgt
    44161 ggccggtgac aagacacctg ccggacgtct gcgcaccggc atcatgtacc tgctggcagg
    44221 acaaatcgcc ggcggggatt ttgtcctgcc gcagtatcag gaggaaatac ggaaaaatct
    44281 cgacccacgc taccatgagg tgattttccg ccgcattgaa cagcttgatc aggaggtcaa
    44341 gaccaaagtc tatgacgaac tgcacaatgc aaaagggatc aatttcatct gggaagccct
    44401 ggacacccag gagctggagc agcgtaagtt cgggatacgc acagtgctga gcacccagct
    44461 actgcaggac tatccaccgg cagttctcaa atccgccaac accctctggt tgctgcgtta
    44521 ccgccctgac gaaattccgt tcctgcggga taattttggt gtccctgaag tcaccctgcg
    44581 gcgcttcctg aaaatgccgg aaggggccgc accggacggc agcggtgtcc cggtactggc
    44641 tgtgttccgt gtaaaaaacg gcacgctggc ccggatcctg aaattcaccc tcggtccact
    44701 ggagctgtgg gcactgaact cctctcctaa agacagcgcc ctgcgacgtg cgctgacgca
    44761 ggaggtgggg tcactgcggg cacgccagat cctggccgaa cactttcccc ggggttcagc
    44821 cacctcactg atagaacacc gcgcccgcac acatgacagt gaaaacgtca tccatgagct
    44881 ggccgcagaa ctcatccgta agcagggata tcacctctga tgtacagcat tcatatcacc
    44941 ccccgctgcc ggcgtcctgc ccggggacag gtcttcctga aaacgctgcg cgtggacggt
    45001 gacagtttca gcttcaccct gccgtccgcc tgcttcacac tgcgaccgtc ccccaatccc
    45061 cgggtcattt ttgccagtga cagacgggct gacggggaga tgagtggtat tcgcctggcc
    45121 ctgcccggag agacaggcac gctgataaca acggccacct ggcaggttaa cgtgccggag
    45181 cgaccgcctt ttgatataca gcatgtcatt cactggcttc tgcccgcacc actcactgcg
    45241 ctgatgtctg acaacacctg gctgtggccg gatagccccg gcacaccggc actgacggcg
    45301 ggagactggc cggtaatgga aaccgatacc ccgacgctga aacgcgtgaa gcgtcgtacc
    45361 ttcacgctca tcgataaccg caaccgtcag ggatggatca cccgtcgctt ccagtgcttc
    45421 ccgctggctc cgctgccgga atcacacagt caccgcgtac cagacatcct gtatcccctg
    45481 aaaacggaga aaaaagaatg caaagacaac cagcaaccac actgcttgca ctcctgatag
    45541 gctgtacggt tatcccgtcc acccacgcct atcctgtgac cgtagtggcc agccagcctg
    45601 tcaccacaca aatcatgccg gagctggccg ccattcaggg cgtgatggca gagatggtcc
    45661 gtatgcagac agccaccggc acagccatca accagagttc agagaagctg gcctctgtca
    45721 tcgcacagga tgggcaggcc acccgtcagc agatgatatt cagtaatgaa acccgccggc
    45781 ttgaggaagc ccggaagagc ttcagcgtgc cggactccat ctgcagtgaa tccgcctccg
    45841 gagttgcagc tgaaagtcgc cgtgcagcag cgtctgcgtc tgcacgcctg agtcagggag
    45901 gaggcgtcag cagtaaaccc atccgtgaac gtctctcccg ggcagcagac tcaccaaccc
    45961 gcgaggcata tgacagtgca ggcattcatg ccggttactg cacggaagct gaatatgccc
    46021 gctttggcgg aaccgatgtc tgccctgccg tgggcgagct gccgggcgga gacagccagg
    46081 tgcgatcgct ctatcagggt gcaggcacag ctgatacccc tgccgccctc acctgggatc
    46141 agaaacagat tgatgctgcc acggcttaca tgaaaaacac cgccagaccg tcagcgggtc
    46201 gtgccccggg gaaaggtgaa gtgggaacac agaccgggcg cacgtatgtg ggcctgcaga
    46261 atgaatacaa cggcatcatc gatgccgcct ctcatccgca actgtcactg atagctgaca
    46321 gcacccctaa tgaggccacc cgtggagcgc tgactgaagc cctgcagtca ccatccgctg
    46381 cggcatattt tgaccggacc gcatcatcag aggcccgcac ccgtggtcac atgagccagc
    46441 gggagtttga ggcctttgaa gctggcagac gctatgcaaa caccgactgg cagcaggatc
    46501 tgcagggcat ggaaggcgat aaccttctcc gtgaactgct gcgcactacc gccctgctga
    46561 actggcagat gaacgacctg aaagagcaga tacgccaggg taatgttatc gccgggcagc
    46621 agctggcact ggcggcacga cagtattacg gacagcgtct aagtgaactg tcacaggcca
    46681 tgagccaggg gagcgtaaga tgagagacgg caacagcacc gacgaaaaaa aaacggccac
    46741 ccgtctccgc cgcatcggtg acatcgggat aaacctgttc gcaccactgg cagaaacccg
    46801 gtggatgctg cgcagctgcc gccatatgct gggccagcat aaaacgcgcc tgaagcgcct
    46861 catgcccgac cggcaaccgg agagtccggt ctgtatgacc tgggaagaag ccgtcagggc
    46921 cagcggcctt acggtcaacg aactggatcg gcgtctgcga cgtcgcagga tgttctggcg
    46981 gacaggattc tggctgatga tgataccggt acttctgctg tgcggcatgg cccttgctgc
    47041 cacagcgctt ccggccatca ccctgatacg tatactggcc tgcctgttac tgatgagtgc
    47101 tattgcagca ctgtgtgcct caaaaaccct gattgtgtca taccgcctct ggcaactgaa
    47161 tgaacggcgg gtcagcgagg acgaacaggg caccttcccc gattacctgc aggcccggca
    47221 cggctggaaa caggccacat ggctggccat tacccaccgg cagtactgac cgtccggtct
    47281 gcaattccct ttcctcattc cggaggctga tgtcctgcat cccttcggtt ccggagttat
    47341 cgtgaaaatc atacccagag cactgagtgc aggtctggcg ctggctgccg tacctgcaca
    47401 ggcattcgtc acctatcagg acattgtgag cgccgccacc aatccggatg acctgtcgcg
    47461 ccaggcactg gtcaccattt ttggcgacgt ggtcacaaat cccctgacag ccagcaacaa
    47521 caccattatc ggtaacctgt tcatggtgtt taacgcaatt atcgccatta tggggatagt
    47581 ctggttcctg tttattggaa tccgccatgt tgtccgcacc ggccaccagg gcaatgtctt
    47641 taacaacgga cgggacgtcg tcggaatgct cagtattgtg agcggtttta tgatgattgt
    47701 gcccaccgct tccggctgga gcctggcaca actggtcatg ctctggggcg catccatcat
    47761 gggcattggt tccgccaata tcatggttca gacggctgca gaaaacatcg ctgaaggcta
    47821 ttcaatgacc gtacagccag ttcatgtctc cacccgcacg gcagcacggg gaatactgga
    47881 gatggcgctg tgcaaacacg ccatgaatgc aggcctcgat gactttaacc gtactgcccg
    47941 ttcatcaacg ccccccatga gcgaatcctc acggacagag aacggcaggt ataccgtcac
    48001 cgtttcaaat ggcagtgcca cctgcgggag tgtgagtctc accgtgaaag ggaatggcag
    48061 caacaaccag tccgcactgg actatttttt caatcccttc attgaaacgg agtatcaggg
    48121 tgtcctgacg gcacaacggt ctgccatgga taccatgctc cgtgacctgg acagcgcagc
    48181 acaggaattt gtgtcagcgt accggacccg acgcgacacg ggtaacggca acctgccgga
    48241 tattgaaagc cgtattcaga aggctgccaa caggtatgaa gaaacggtgc agaaggcgtt
    48301 gccaacagat aacgcagaaa ctgagcgcag ggagcagctg aaatcctacc tgaccaccta
    48361 cggctgggtt gccctggggg cctggtacca gacctttgcc actgccaacc agcgactggc
    48421 agaactggcc gaccgtgccc ccactgtgag tggagtttca tcgcagggcg aagcagggga
    48481 tactgaattc ctgaaggaag taaaaggagc ttaccaggca cagttgcaaa actcaccata
    48541 tacacctgca ctgggaacag ccacctcagc cagtgaatac caggcatcta atacacagaa
    48601 tcctcaatca gccctgttaa aactcctagg gaatgacctg acgttattta ccaatcaaat
    48661 tgcaaccata cattcaggaa ctggaaccac ctcggaccaa ctaaatccgc taataaaaat
    48721 gaaaaacatt ggtgattaca ccatggtcac aaccgaaacc atctgggcgg gttataccac
    48781 cgtcagggta ctttccactg ctggcagcaa ctccattttt ggtcgactgg tcaatgctgt
    48841 tagcggctta tctgacgccg caaaggcttt tctggaagca tccgcaccac cgctctactt
    48901 cctgctgttt ttgctgttct gtgccggttt ttcgttatcc atttacctgc cgtttattcc
    48961 gtttatcttc tggatgaccg gcgtcggtaa ctggatagtc agtgtactga ttggatgcgc
    49021 cgccggcccc ctgtgggccg caacccatct gggaacaagc caggacaggg gaagccgtgc
    49081 tgcttatggt tacatctacc tgatagacag tatgatcaga ccactgctga tggtgttcgg
    49141 gttctttttt gcatcggtgg ccatcatggc cggcggcaca atactgaatg cgctgtttgc
    49201 cgccgcgctg atgaatgtcc agataaattc tgtgacgggc gtgttcagca tggcaggatt
    49261 tttgatgatt tatgcccgcg tctgtaccac gctggttgcc gccgtttttg cacttcaggc
    49321 ttatctgcct gaccatgtaa ttaattttct gggcggacgt gacggcgtta atatgctggg
    49381 gaatatggtt tcttcagtca aagatattat tgccggaggt aataccaata tgcgcagaac
    49441 acctggcgtt cgtgaagaaa aactgaaaaa tattaatgga gatgataagg acggaataaa
    49501 acaatagagt aaaaacatca aaaataaata aagcagtatt gccatattca cctgaagaga
    49561 ttaaaatata ttcaggtgaa tatgggggtt tattatgaac aaaacaaaaa gcagatatcc
    49621 ttcctggtgg gcatattatc aactagcccg caacgcttat tttttattag gcattccgtg
    49681 cttagcttta tacgcaacag cttatacatc atatttatta gatgataaac taacgcattc
    49741 aacaagcctt tttattctgg catatggatg ttattttctt cttttcattc ctccgctctg
    49801 gctatatctg agaaccagag cgaaaaaaaa gaaagtccag caggttgttc agcagataaa
    49861 agaaagcagt ccctttgcgc cgaccagcga ttacgagcaa ctgtcattct cgaagagttg
    49921 ttatttcggg atcgacatta aaaacggcac catgctgtat gtgcgcattt acccaaacaa
    49981 tgtcatggat gtgattggcc tggatattca caactttacc cgcacggttg ctgaagacgg
    50041 caagctggaa atccacacca cctatgtgag tctgccgatg atccctcttg aaatctccgg
    50101 catctcatca aggacactgg ccaacaccat gcacaccatg gctgcacgtg gatatgagta
    50161 caaggaacgt ttcccgcaga tgatccggta tcgggttaaa gagtgggaaa aagttgccgg
    50221 tgtgcctgtt gcagaagtat tttaaggtag cgaccaactg ttagacggta atccgtcagc
    50281 cggttttctc tgaaccggct ttttcttttc aggcagaatg cctgtctgcc gtctatctca
    50341 ggacgccgca tggagtctct attttttccc cacaaattct tagcactgtc ttgcccactg
    50401 tagacaaaat caatacaatt ctgcatatca gttatctata aaagataaca atatcattat
    50461 aaataattat catatccaca cactcaaggc agtacctaga aaacataaag ataaaatcat
    50521 gtctgaatcc cgcagcaatt ttccagcacg acatggtact ccatggaata ctgaagagtt
    50581 acagcgtctt ctcgtatcca tagcggaggg cgtctcgcta aaaaaactta ctcaaacaca
    50641 tcaacgatcg tccgggggaa tacgtggagc aatcaaacgc ctgcttcctt ctgagtttct
    50701 tgagttcaac gatataaaca ctgtgaatga tctcagcaat tacttcaaca agattcctcg
    50761 ccaagaacac aatagta
//
LOCUS       DAZGVQ010000014        49859 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000014 DAZGVQ010000000
VERSION     DAZGVQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.014"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            41..1480
                     /gene="norV"
                     /locus_tag="ACHLAG_000791"
     CDS             41..1480
                     /gene="norV"
                     /locus_tag="ACHLAG_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFP0654072.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            1477..2610
                     /gene="norW"
                     /locus_tag="ACHLAG_000792"
     CDS             1477..2610
                     /gene="norW"
                     /locus_tag="ACHLAG_000792"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFP0654073.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(2738..4990)
                     /gene="hypF"
                     /locus_tag="ACHLAG_000793"
     CDS             complement(2738..4990)
                     /gene="hypF"
                     /locus_tag="ACHLAG_000793"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFP0654074.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAATQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(5143..5670)
                     /gene="hydN"
                     /locus_tag="ACHLAG_000794"
     CDS             complement(5143..5670)
                     /gene="hydN"
                     /locus_tag="ACHLAG_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFP0654075.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(5819..6829)
                     /gene="ascG"
                     /locus_tag="ACHLAG_000795"
     CDS             complement(5819..6829)
                     /gene="ascG"
                     /locus_tag="ACHLAG_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFP0654076.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSVERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            7089..8546
                     /gene="ascF"
                     /locus_tag="ACHLAG_000796"
     CDS             7089..8546
                     /gene="ascF"
                     /locus_tag="ACHLAG_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFP0654077.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            8555..9979
                     /gene="ascB"
                     /locus_tag="ACHLAG_000797"
     CDS             8555..9979
                     /gene="ascB"
                     /locus_tag="ACHLAG_000797"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFP0654078.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(10093..10512)
                     /locus_tag="ACHLAG_000798"
     CDS             complement(10093..10512)
                     /locus_tag="ACHLAG_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0654079.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(10583..11338)
                     /locus_tag="ACHLAG_000799"
     CDS             complement(10583..11338)
                     /locus_tag="ACHLAG_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654080.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     EQNWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGVLQNMAGNAQLYRAD
                     LDRNGIQDLVIWRGISGNGLAPNAFLILMTFNQQGRPCVFQSDGFYTASETGIDDLLD
                     LQRNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYTPNRKLV
                     LKPIAGRDPQTEDLAQTQRCLIKGDVLYGVNQN"
     gene            complement(11381..11851)
                     /gene="hycI"
                     /locus_tag="ACHLAG_000800"
     CDS             complement(11381..11851)
                     /gene="hycI"
                     /locus_tag="ACHLAG_000800"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFP0654081.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEVE"
     gene            complement(11844..12254)
                     /gene="hycH"
                     /locus_tag="ACHLAG_000801"
     CDS             complement(11844..12254)
                     /gene="hycH"
                     /locus_tag="ACHLAG_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFP0654082.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(12251..13018)
                     /gene="hycG"
                     /locus_tag="ACHLAG_000802"
     CDS             complement(12251..13018)
                     /gene="hycG"
                     /locus_tag="ACHLAG_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFP0654083.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(13018..13560)
                     /gene="hycF"
                     /locus_tag="ACHLAG_000803"
     CDS             complement(13018..13560)
                     /gene="hycF"
                     /locus_tag="ACHLAG_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFP0654084.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(13570..15279)
                     /gene="hycE"
                     /locus_tag="ACHLAG_000804"
     CDS             complement(13570..15279)
                     /gene="hycE"
                     /locus_tag="ACHLAG_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFP0654085.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(15297..16220)
                     /gene="hycD"
                     /locus_tag="ACHLAG_000805"
     CDS             complement(15297..16220)
                     /gene="hycD"
                     /locus_tag="ACHLAG_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFP0654086.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(16223..18049)
                     /gene="hycC"
                     /locus_tag="ACHLAG_000806"
     CDS             complement(16223..18049)
                     /gene="hycC"
                     /locus_tag="ACHLAG_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFP0654087.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGSAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(18046..18657)
                     /gene="hycB"
                     /locus_tag="ACHLAG_000807"
     CDS             complement(18046..18657)
                     /gene="hycB"
                     /locus_tag="ACHLAG_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFP0654088.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(18782..19243)
                     /gene="hycA"
                     /locus_tag="ACHLAG_000808"
     CDS             complement(18782..19243)
                     /gene="hycA"
                     /locus_tag="ACHLAG_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFP0654089.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYYNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            19455..19805
                     /gene="hypA"
                     /locus_tag="ACHLAG_000809"
     CDS             19455..19805
                     /gene="hypA"
                     /locus_tag="ACHLAG_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP0654090.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            19809..20681
                     /gene="hypB"
                     /locus_tag="ACHLAG_000810"
     CDS             19809..20681
                     /gene="hypB"
                     /locus_tag="ACHLAG_000810"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFP0654091.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIAYAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            20672..20944
                     /gene="hypC"
                     /locus_tag="ACHLAG_000811"
     CDS             20672..20944
                     /gene="hypC"
                     /locus_tag="ACHLAG_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFP0654092.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            20944..22065
                     /gene="hypD"
                     /locus_tag="ACHLAG_000812"
     CDS             20944..22065
                     /gene="hypD"
                     /locus_tag="ACHLAG_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFP0654093.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            22062..23072
                     /gene="hypE"
                     /locus_tag="ACHLAG_000813"
     CDS             22062..23072
                     /gene="hypE"
                     /locus_tag="ACHLAG_000813"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFP0654094.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDMLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            23146..25224
                     /gene="flhA"
                     /locus_tag="ACHLAG_000814"
     CDS             23146..25224
                     /gene="flhA"
                     /locus_tag="ACHLAG_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFP0654095.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(25261..25614)
                     /gene="ygbA"
                     /locus_tag="ACHLAG_000815"
     CDS             complement(25261..25614)
                     /gene="ygbA"
                     /locus_tag="ACHLAG_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFP0654096.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(25646..25825)
                     /locus_tag="ACHLAG_000816"
     CDS             complement(25646..25825)
                     /locus_tag="ACHLAG_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654097.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVG
                     EPFFFRVIVPFKIKM"
     gene            25901..28462
                     /gene="mutS"
                     /locus_tag="ACHLAG_000817"
     CDS             25901..28462
                     /gene="mutS"
                     /locus_tag="ACHLAG_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP0654098.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            28568..29224
                     /gene="pphB"
                     /locus_tag="ACHLAG_000818"
     CDS             28568..29224
                     /gene="pphB"
                     /locus_tag="ACHLAG_000818"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP0654099.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFYPETD
                     LLISTGDNIDRGPKSLNVLRLLNQPWFTSVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(29275..30042)
                     /gene="ygbI"
                     /locus_tag="ACHLAG_000819"
     CDS             complement(29275..30042)
                     /gene="ygbI"
                     /locus_tag="ACHLAG_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFP0654100.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASLALAKQDLSLLVAKNE"
     gene            30238..31146
                     /gene="ltnD"
                     /locus_tag="ACHLAG_000820"
     CDS             30238..31146
                     /gene="ltnD"
                     /locus_tag="ACHLAG_000820"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFP0654101.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            31143..32405
                     /gene="otnK"
                     /locus_tag="ACHLAG_000821"
     CDS             31143..32405
                     /gene="otnK"
                     /locus_tag="ACHLAG_000821"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFP0654102.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            32402..33040
                     /gene="ygbL"
                     /locus_tag="ACHLAG_000822"
     CDS             32402..33040
                     /gene="ygbL"
                     /locus_tag="ACHLAG_000822"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFP0654103.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            33045..33821
                     /gene="ygbM"
                     /locus_tag="ACHLAG_000823"
     CDS             33045..33821
                     /gene="ygbM"
                     /locus_tag="ACHLAG_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFP0654104.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            33910..35274
                     /gene="ygbN"
                     /locus_tag="ACHLAG_000824"
     CDS             33910..35274
                     /gene="ygbN"
                     /locus_tag="ACHLAG_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFP0654105.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(35313..35549)
                     /locus_tag="ACHLAG_000825"
     CDS             complement(35313..35549)
                     /locus_tag="ACHLAG_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFP0654106.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(35560..36987)
                     /locus_tag="ACHLAG_000826"
     CDS             complement(35560..36987)
                     /locus_tag="ACHLAG_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFP0654107.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNAPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGDEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(36987..37580)
                     /locus_tag="ACHLAG_000827"
     CDS             complement(36987..37580)
                     /locus_tag="ACHLAG_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFP0654108.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            37727..38134
                     /locus_tag="ACHLAG_000828"
     CDS             37727..38134
                     /locus_tag="ACHLAG_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP0654109.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(38255..39247)
                     /gene="rpoS"
                     /locus_tag="ACHLAG_000829"
     CDS             complement(38255..39247)
                     /gene="rpoS"
                     /locus_tag="ACHLAG_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFP0654110.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLVGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(39310..40404)
                     /gene="nlpD"
                     /locus_tag="ACHLAG_000830"
     CDS             complement(39310..40404)
                     /gene="nlpD"
                     /locus_tag="ACHLAG_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFP0654111.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(40589..41215)
                     /gene="pcm"
                     /locus_tag="ACHLAG_000831"
     CDS             complement(40589..41215)
                     /gene="pcm"
                     /locus_tag="ACHLAG_000831"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFP0654112.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(41209..41970)
                     /gene="surE"
                     /locus_tag="ACHLAG_000832"
     CDS             complement(41209..41970)
                     /gene="surE"
                     /locus_tag="ACHLAG_000832"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFP0654113.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(41951..43000)
                     /gene="truD"
                     /locus_tag="ACHLAG_000833"
     CDS             complement(41951..43000)
                     /gene="truD"
                     /locus_tag="ACHLAG_000833"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFP0654114.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(42997..43476)
                     /gene="ispF"
                     /locus_tag="ACHLAG_000834"
     CDS             complement(42997..43476)
                     /gene="ispF"
                     /locus_tag="ACHLAG_000834"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFP0654115.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(43476..44186)
                     /gene="ispD"
                     /locus_tag="ACHLAG_000835"
     CDS             complement(43476..44186)
                     /gene="ispD"
                     /locus_tag="ACHLAG_000835"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFP0654116.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(44205..44516)
                     /gene="ftsB"
                     /locus_tag="ACHLAG_000836"
     CDS             complement(44205..44516)
                     /gene="ftsB"
                     /locus_tag="ACHLAG_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFP0654117.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(44710..45033)
                     /gene="ygbE"
                     /locus_tag="ACHLAG_000837"
     CDS             complement(44710..45033)
                     /gene="ygbE"
                     /locus_tag="ACHLAG_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFP0654118.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(45083..45688)
                     /gene="cysC"
                     /locus_tag="ACHLAG_000838"
     CDS             complement(45083..45688)
                     /gene="cysC"
                     /locus_tag="ACHLAG_000838"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFP0654119.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(45688..47115)
                     /gene="cysN"
                     /locus_tag="ACHLAG_000839"
     CDS             complement(45688..47115)
                     /gene="cysN"
                     /locus_tag="ACHLAG_000839"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFP0654120.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIRKDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAVEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(47117..48025)
                     /gene="cysD"
                     /locus_tag="ACHLAG_000840"
     CDS             complement(47117..48025)
                     /gene="cysD"
                     /locus_tag="ACHLAG_000840"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFP0654121.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            48277..49314
                     /gene="iap"
                     /locus_tag="ACHLAG_000841"
     CDS             48277..49314
                     /gene="iap"
                     /locus_tag="ACHLAG_000841"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFP0654122.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   49335..49790
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=49396..49424
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
BASE COUNT        11768 a        13605 c        13081 g        11405 t
ORIGIN      
        1 agacatcttt ttagaacacg ctgaataaat tgaggttgct atgtctattg tggtgaaaaa
       61 taacattcat tgggttggtc aacgtgactg ggaagtgcgt gattttcacg gcacggaata
      121 taaaacgctg cgcggcagca gttacaacag ctacctcatc cgcgaagaaa aaaacgtgct
      181 gatcgacacc gtcgaccata aattcagccg cgaatttgtg cagaacctgc gtaatgaaat
      241 cgatctggcg gatatcgatt acatcgtgat taaccatgca gaagaggacc acgctggggc
      301 gctgaccgaa ctgatggcgc aaattcccga tacgccgatc tactgtactg ccaacgctat
      361 cgactcgata aatggtcatc accatcatcc ggagtggaat tttaatgtgg tgaaaactgg
      421 cgacacgctg gatatcggca acggcaaaca gctcattttt gtcgaaacac caatgctgca
      481 ctggccggac agcatgatga cttacctgac aggcgacgcg gtgctgttca gtaacgatgc
      541 tttcggtcag cactactgcg acgagcatct gttcaacgat gaagtggatc agacggagct
      601 tttcgagcag tgccagcgtt actacgccaa tatcctgacg ccgttcagcc gcctggtaac
      661 gccgaaaatt accgagatcc tgggctttaa cttaccagtc gatatgatag cgacctccca
      721 cggcgtggtc tggcgcgata acccgacgca aattgtcgag ctgtacctga agtgggccgc
      781 tgattatcag gaagacagaa tcaccatttt ctacgacacc atgtcgaata acacccgcat
      841 gatggctgac gctatcgccc aggggattgc ggaaaccgac ccacgcgtgg cggtgaaaat
      901 tttcaacgtc gcccgaagcg ataaaaacga aatcctgacc aatgtcttcc gctcaaaagg
      961 cgtgctggtc ggcacttcga cgatgaataa cgtgatgatg ccgaaaatcg ccgggctggt
     1021 ggaggagatg actggtttac gcttccgtaa caaacgcgcc agtgctttcg gctctcacgg
     1081 ctggagcggc ggtgcggtgg atcgtctttc cacgcgcctg caggatgcgg gtttcgaaat
     1141 gtcgcttagc ctgaaagcca aatggcgacc agaccaggac gctctggagt tatgtcgtga
     1201 acacggtcgc gaaatcgccc gtcagtgggc gctcgcgccg ctgccgcaga gcacggtgaa
     1261 tacggtggtt aaagaagaaa cctctgccac cacgacggct gacctcggcc cacggatgca
     1321 gtgcagcgtc tgccagtgga tttacgatcc ggcaaaaggc gagccaatgc aggacgttgc
     1381 gccaggaacg ccgtggagtg aagtcccgga taacttcctc tgcccggaat gctccctcgg
     1441 caaagacgtc tttgaagaac tggcatcgga ggcaaaatga gtaacggcat tgtgatcatc
     1501 ggttcgggct tcgccgcccg ccaactggtg aaaaatattc gcaaacagga cgccactatt
     1561 ccattaaccc tgattgccgc cgacagcatg gatgagtaca acaaacctga cctcagccat
     1621 gttatcagtc aggggcaacg tgccgatgac cttacccgcc agacggcggg tgaatttgcc
     1681 gagcagttta atctgcacct gtttccacaa acctgggtga cggatatcga tgccgaagcc
     1741 cgtgtggtga aaagccagaa taatcagtgg caatacgaca agctagtact ggcaaccggt
     1801 gccagtgcct ttgtcccgcc tgtgcctggg cgtgagttaa tgctgacgtt aaatagtcag
     1861 caagagtatc gcgcctgtga aacgcaactg cgggatgccc gacgcgtgtt gattgttggc
     1921 ggtggtttga ttggtagcga actggcgatg gatttttgtc gtgcaggcaa agcggtcacg
     1981 ctaatcgaca acgctgccag tattctggcg tcgttaatgc caccggaagt aagcagccgc
     2041 ttgcagcatc ggttgacgga gatgggcgtt catctgttgt tgaaatctca gttgcagggg
     2101 ctggaaaaaa cagattctgg cattctggca acgctggacc gccagcgcag tatcgaagtg
     2161 gatgcggtaa ttgccgccac cggactgcgc ccggaaaccg ccctggcacg acgcgccggg
     2221 ctgacgatta atcgcggcgt ttgcgtcgat agttatctgc aaaccagtaa tgccgatatt
     2281 tacgcgctgg gcgattgcgc ggaaattaac ggtcaggtat tgccgttcct ccagccgatt
     2341 caacttagcg cgatggtgct ggcaaaaaat cttctcggca ataacacgcc actgaaacta
     2401 ccggcgatgc tggtgaaaat caaaacgccg gaattaccgc tgcatctggc aggcgaaacc
     2461 cagcgtcagg atttacgctg gcaaattaat accgaacgcc agggaatggt ggctcgcggt
     2521 gttgacgatg ctgaccagct tcgcgccttt gtggtcagtg aggatcggat gaaagaggca
     2581 tttggattgt tgaaaacatt gccgatgtag gtgggctact gtgcctaaaa tgtcggatgc
     2641 gacgctggcg cgtcttatcc gacctacggg gacgcatgtg taggccggat aaggcgttta
     2701 cgccgcatcc ggcaatggtg tccaaatgca acacgtttta tccgttctgg acttcacccg
     2761 ctaaccaacg cgccgcagca ataaccccct gccccagaga caaaccgcca tcacccgccg
     2821 gtaaactctg tggaaagagc aatgtgaaat cagcgagata atgcgccaga cgtgcacgca
     2881 gcaaacggtt atgaataacc ccgccgctaa ataccagcgt agtgatacca cgcatcgtgg
     2941 cctgctcacg catcaacgcg gcaaaaccct gcgccagcgc atcatgaaac gcccacgcgc
     3001 gttgattaac cggtgcctgc cagttcagcc actgctgcca gaaagtggcg agatccagtt
     3061 gattgtccac cagcggcatt gtcaccggat gcgtcactcc gtggcacgaa gctgcgagtg
     3121 cctccagagc acaagccgct tcaccttcat aacttaacgt ggctggcgca cagcccagtg
     3181 ccgccgccac ggcatcgaac aaccgcccac acgatgacgc cagcggcgcg ttaattccac
     3241 gctcaatggc ccgcgccagc acgctccagt tttgctgttg cacacttgct gtttcagaat
     3301 aattctgcca ctccggcaca aagcgcaggc actgcgccag caggtttcgc cacggctgcg
     3361 tcgccgccaa atcgccaccc ggaagcgcca ctgcaggcaa gccgcccagg tgctcacatt
     3421 cgcgatagtt cacccgcaga cactcgccgc cccacaaagc gccgttctcc cccataccga
     3481 taccatcgag cgtcaaagca atgacatcac cgccatccag cggccactga tgctctgcca
     3541 gacacgccgc tgcgtgggca tgatgatgca gcaccgtttg cgtcggcata ttcatttcgc
     3601 tcgcccactg gctggagaca tagcccggat gcacgtcatg cacaacgtat tgcggagtga
     3661 aatcgtagat gttttgcatc aggcgtaacg cttcgcgcca ctgcatctgg atgccatcgt
     3721 cacttaaatc gcccagatgc tgactcaaca ccgcttgttc accgcgcacc aggcagaagg
     3781 tgtttttcag atccgcgcca agacacagca caggcggaac atttttaaag cccggaggca
     3841 aaaccagcgc atccggcaca tacccgcgtg aacggcgcag catttcgccg ctttcgcgca
     3901 ccaccgaatc atccatccgc tgcacgatgt cgcggttatg tatcaagaat ccgtcggcaa
     3961 tgccctgcaa atccgccagc gcctgttcgt tgctgatagc tggtggttta ccgctcaggt
     4021 tgccggaggt catcaccagc gggcattgca gttcctgtaa cagcaaatgc tggagcgggt
     4081 tcgcaggcaa cattaccccg acttcgttaa ggtcaggggc gatatcatca caaagctcag
     4141 gaacgtattt tttatccacc agcacaatcg gcgcggcggg cgtggtaagc aactggcgcg
     4201 cagcgtctgg taaaccgtca gccactggca acatgaccgc cagcggtttc gccgggcgat
     4261 gtttgcgcgc ccgcagtgtc gccaccgcgt tactgttacg tgcatcgcag gcaagatgaa
     4321 atccgccaat ccctttgatg gcgacaatgt tgcccatttt taactgtgcg atagccgcct
     4381 gtaatgccgc ctcttgttct gcatgttcac catgacttac ccattcaaga tgtgggccac
     4441 actccgggca ggccaccggc tgggcgtgga agcgacgatc gagcgggtcg cggtactctt
     4501 tgtcacaagc cggacacaac ggaaacgccg ccatcacggt aaacgggcgg tcgtaaggca
     4561 tggcgcgaat aatggtgaaa cgcgggccgc agtgggtaca gttgataaac ggataacgat
     4621 aacgccgttc gcctggggta ttcatttcgg caagacaggc agggcaagta gcggcatcgg
     4681 gaacaatttg cgtattcatg gtgccgcctg tgctctggcg tatagtgaac tcggtgggca
     4741 gttgtgacca gatgtacggc tcacgctcga cgctatcaat acgcgccagc ggcgggcagt
     4801 gctgatgcaa ttgaacaaga aacgtttccg ggtcttccag cagccggact tctacgccat
     4861 cgccgtcatt acagacatcg ccgtgaagat ttaattgctg tgccagctgc cagacaaacg
     4921 gacgaaaacc gacgccctgc actttgcctc gaatacgcag ttggacaccg caagatgtgt
     4981 tttttgccat tgagttattc ccgccatcat gaattgcgta acccgccctg ccggacacga
     5041 ctgcgtcgta tccggcagtc tcaggtcggc gataccgcca gctccgtatt ctacgaatat
     5101 ttccgggaat tcctttgatg ccagaacagt tctataagat ttttagaaca tcagcgccgt
     5161 acggcgacgt ttttctgcgc tcagttgttc aagtttatta cgatcgacac aaatcagcgc
     5221 atgggtcggg caagccgcca tacacgccgg gccgtcttcg cgatggttgc acaggtcgca
     5281 tttattggct tcagctttgt cagcccgtac attcagcccc gcgccgctgt tgcggatcac
     5341 cggacgtacc accacttcca tcgcgccata cgggcaagcc accacgcagg ttttgcaacc
     5401 aatgcaacgt tcctgcatca catgaacaaa ccctttatca cggctgatag caccattcgg
     5461 gcagacgtta gcgcacggtg catcttcaca ctgacggcaa actgtcgccg tggaaatgtt
     5521 cacaccttta atgacatgga tacgcggtaa aaatgtttcc ggggtcagcg atgcacagtc
     5581 ctgattttcc tgatgagaaa ccacgcacgc cacttcacag gtacggcaac caatacattt
     5641 actcgcgtca gcaatgatga aacggttcat caaattctcc agcaatgaca gttaatgcgc
     5701 cgatacattc acaaatcatg ccagttttta attcactgtt atttaaggaa attaatttct
     5761 gtaatgcagg aaaaaagatg tcatcgacac tagtgacgat gacatgtgat gacaatgttt
     5821 atcgcgaaag agcaatgagt gagtcgcggc ggatcagttt tccgctgaag gttttcggcg
     5881 gtgagaaatc cccgccatcg agcataaaaa tcagccgtcc aataatttcc tgaatcatct
     5941 ctgttaccgg aatttttacg ctggagagcg ccggaacggt gtagggggca attgcgatat
     6001 catcgaatcc gataaccgac acctgctctg gcaccgctac accgcgctcg tgcaacgctt
     6061 tgatcgcacc tatcgccata tcgtcgttac tggcaactaa cgcgctaaat ttagccccac
     6121 gttcgagcaa cgtttctacc ccttcggccc cgctggcggg cgtccattta ccgttagcga
     6181 taagtttttc attgagcgca ataccatgct gcgacagcgc gtctttatac ccggcaagac
     6241 gttcaacgct ggtgggggaa tccatcgagc cggtaaggaa agcaatctcc tgatgtccgg
     6301 cgtttatcaa ctctgccacg gcattaaagc tggtctgttt atgatcgcac cagacgctat
     6361 ggctgctgtt tttgcgcagg cggcgattaa gcaccattat cggctgactg tgcgcgtcaa
     6421 tgatgtcatc gatctcatcc acgcttaaaa aacgcgggta aatcataatg gcgtcgcagc
     6481 gcaaatccag cagatactga atcgcctggc gctcttcttc ggcgctgtgt ttaccatctg
     6541 ccaatagcaa ctgccgccct ttctcttccg ccattcgcgc ggcatgaaag agtaattcac
     6601 taaaataaat gccgtggtaa agcgtgttgg tcactaccag ccccagcgtc tgagtactct
     6661 tcgccgacag attgcgcgcc agcaagtttg ggcggtaacc gctctcttct accgcctgaa
     6721 acacgcgatc tttagtctcc tggctgacgt agccattacc tgaaagcacg cgggaaacgg
     6781 tcgcttttga aaccccggcg cgcttcgcca cttccagcat cgtcgtcatc attttcatcc
     6841 ctttacacgc aatcaacgca gtgtactgca ccgtttgccg attgtccttg cgcaatcggc
     6901 gggaaaaata ttcaggtgac cggtttcgca aatataaaaa atgaacaatt aactctcttg
     6961 cttatttagt gacaactatt catgattttg tgaaaccggt ttcctaattc cgtttcagca
     7021 tcggcatttt tccgttacgt cgactgataa caactacatc taccctactg ataacaggat
     7081 aaaatccgat ggccaaaaat tatgcggcgc tggcacgctc ggtgatagcg gcactgggcg
     7141 gcgttgataa catctcggca gtcacgcact gtatgacgcg tttgcgcttt gttatcaaag
     7201 atgaccagct tatcgacagc ccgacgttaa aaaccatccc cggcgtgctc ggcgtggtac
     7261 gtagtgacaa ccagtgtcag gtgattatcg gcaataccgt ttcacaagcc tttcaggaag
     7321 tcgtcagcct gctgccggga gatatgcagc ccgcacagcc cgtgggtaaa cccaaactca
     7381 cgctacgtcg cattggtgcg gggatcctcg atgcgctgat cggcaccatg tcaccgctga
     7441 tcccggcgat tatcggcgga tcgatggtca aactgctggc gatgatcctc gagatgagcg
     7501 gcgtgctgac aaaaggatcg ccgaccttaa ccattctgaa tgtgattggt gacggtgctt
     7561 tcttcttcct gccgctgatg gtcgcggctt cggccgccat caaatttaaa accaatatgt
     7621 cgctggcgat tgccatcgcg ggtgtgctgg tacatccgag ttttattgaa ctgatggcga
     7681 aagcggccca gggtgaacat gtcgaatttg ccctgatacc ggtcaccgcg gtgaaataca
     7741 cctacacggt gatcccggcg ctggtgatga cctggtgcct gtcatatatc gaacgctggg
     7801 tggatcgcat tacaccggcg gtgaccaaaa acttcctcaa gccaatgctg attgtgttga
     7861 ttgccgcacc gctggcaatc ctgctgattg gcccgattgg tatctggatc ggtagcgcca
     7921 tttcggcgct ggtttacacc attcatggtt atctgggctg gctttcagtc gccattatgg
     7981 gcgcgctgtg gcctctgctg gtaatgaccg ggatgcaccg cgtctttacg ccaaccatca
     8041 ttcagaccat tgccgaaacc ggcaaagaag ggatggtcat gccgtcagag atcggcgcta
     8101 acctgtcgct gggcggttca tcactggcgg tggcgtggaa aacgaaaaac ccggaactgc
     8161 gccagacggc gctggctgcg gcggcatcag ccattatggc ggggatttcc gaaccggcgt
     8221 tatacggcgt ggcgatccgc ctgaaacgtc cgcttatcgc cagtcttatc agcggtttta
     8281 tttgcggcgc ggttgccggt atggcggggc ttgccagcca ctcaatggca gcgccggggt
     8341 tatttaccag cgtgcaattc ttcgatccgg cgaatccaat gagcatcgtc tgggtgttcg
     8401 cggtcatggc gctggcggtg gtgctgtcgt ttatcctcac actattgctc ggctttgagg
     8461 atattcctgt tgaggaagcg gctgccgagg cgcgaaagca tcagagcgca caaccgaccg
     8521 tcgccaaaga agtaagtctt aattgaggat gaaaatgtca gtatttccag aaggtttttt
     8581 atggggcggc gcgcttgccg ccaaccagtc tgaaggtgcg ttccgtgaag gtggcaaagg
     8641 actgaccacg gtcgatatga tcccgcacgg cgagcatcga atggcggtga aactggggct
     8701 ggaaaaacgt tttcagttgc gcgatgacga gttttatccc agccatgagg cgacggattt
     8761 ttatcatcgt tataaagaag atatcgccct gatggcagag atgggattca aggttttccg
     8821 tacctcaatt gcctggagcc gcctctttcc gcagggcgat gaaatcacgc ccaatcagca
     8881 gggcattgct ttttaccgtt ctgtctttga agagtgtaaa aagtacggta tcgaaccgct
     8941 ggtcacgttg tgccacttcg atgtgccgat gcatctggtc accgaatatg gctcctggcg
     9001 taaccgcaag ctggtggagt ttttcagccg ctacgccaga acctgctttg aagcatttga
     9061 tggtctggtg aaatactggc taaccttcaa tgaaatcaac attatgttgc atagcccgtt
     9121 ctccggcgcg ggtctggtgt ttgaagaagg tgaaaatcag gatcaggtga aatatcaggc
     9181 cgcgcatcac cagctggttg ccagtgcgct agccaccaaa atcgcccatg aggttaaccc
     9241 gcaaaatcag gtgggctgta tgctggcggg cggtaacttc tacccttaca gctgcaaacc
     9301 ggaagatgtc tgggcggcgc tggagaaaga tcgggaaaac ctgtttttta tcgatgtgca
     9361 ggcgcgcggc gcgtatccgg cttactctgc ccgcgtattt cgcgaaaaag gggtaaccat
     9421 caacaaagca ccgggcgatg atgaaatcct gaaaaacacc gtcgattttg tctctttcag
     9481 ctattacgcc tcgcgctgcg cctcggcgga gatgaacgcc aacaacagca gtgcggcgaa
     9541 cgtggtgaaa tcgctgcgta atccgtatct acaggtgagc gactggggct ggggaattga
     9601 tccactcggt ctgcgtatca ccatgaatat gatgtacgat cgttatcaga agccgctgtt
     9661 tctggtggaa aacggcctcg gcgcaaaaga tgaacttgct gccaatggcg agattaacga
     9721 tgactatcgc atcagctatt tacgcgaaca tatccgcgca atgggcgaag cgattgcaga
     9781 tggcattccg ctgatgggct acaccacctg gggctgtatt gatttagttt ccgcctctac
     9841 gggtgaaatg agcaaacgct acggttttgt ctacgtagac cgtgacgacg caggcaacgg
     9901 cacgctgacg cgcacgcgta agaaatcgtt ctggtggtat aaaaaagtaa ttgccagtaa
     9961 tggggaagat ttagagtaag taaccgtgcc ggatgcggcg tgaacgcctt atccggccta
    10021 catagttcgt gcgacatgta ggcccgataa gcgcagcgca tcgggcaatc tggggtttgg
    10081 catcagtcaa aatcaaagaa accattccgg cttaacgcca aatcgggctg aaagtgcctt
    10141 aatgtgggat attgtcaggg atcgttgtcc agacaaaatc tgactgacca gggatttaga
    10201 gccgatttcg ttcttcaaat ccgcataggt cagattatgt tgatcaatca acgtgcgaag
    10261 taacgccacg ccaaccggca ttgccgcaac ggctttgtcg aactctgcaa acttttcgtt
    10321 gttgttttca tactctgcaa tgcggctcgc cagaaagtcg ataagtggat tggtgtcatc
    10381 ttcttcaatc aaatattcga ccagttccag tgcttcacga taatcatctt ctgagtcact
    10441 gcctcccaga aaggggactg cattgaccag ttctcttgtt gctttaacag cgcgtgcagc
    10501 attagcagtc attctttgtt ctctctgtag taacgtgacg tctgtctgca cgtaatcccc
    10561 ctcatagcca ctctgcaaaa agtcagttct ggttcacccc ataaagcaca tcccccttta
    10621 tcaagcaacg ctgcgtctgc gccagatctt ccgtttgcgg gtcgcgcccg gcgatgggtt
    10681 tgaggacgag tttacggttg ggggtataag taaaacgcgt gagcgcggga tagcttaact
    10741 tgccaaacca gccgtgaaca cgctgccatt tggcgtcttt tacctgatac agactggtta
    10801 tccagtaacc ggaatcaaac tgcatatcca gcaattgcgt gtggccattc ctttgtaaat
    10861 ccagcaaatc atcaatgcca gtttcacttg cggtataaaa accatcggac tgaaaaacac
    10921 acgggcgacc ttgctgatta aaggtcatca gaatcaaaaa cgcgttaggt gccagaccat
    10981 ttcctgaaat accccgccag ataaccagat cctgaatgcc gttacgatca agatcggcac
    11041 ggtaaagttg cgcattaccc gccatatttt gcagcacccc cacaggcaca gaccaggatt
    11101 taccggcgcg atctttaccg ttcaattgcc attcactatc atcatttggc atcttttcaa
    11161 gcgtcacgcc agcaacgccg acttttctaa aatgccagtt ttgctcggca tcataaggca
    11221 tggtcaggag aggtttcagc gcccgctttt gcgcgtattc gctgtcactc atcggcagat
    11281 attcggtaaa gctggtggcc tgtgcaggca gcactaagct caataaaaaa gcgcacatcc
    11341 ctgttctgat gttcaattcc ctgaattcct taatgaaaaa ctactctact tccaccgcta
    11401 actgcgcaaa tccaccattt ccttcccagc cttccagtcg ttgataaacg gtttcaaccg
    11461 catctttaat cggctgggtc atcgggtagt aaaagccgac gatatccggc tggatgccga
    11521 ggaaaatcac ttcgccaata tcttctttca actggtcgat aaggtagttc aacggcatgt
    11581 tatgggtagt catcataaac atctcggcga tatcatccgg gtcgatgatg cggatctcgc
    11641 cggggtttag ccccatatca gtggcgtcga caatcagcag tcgcgtaggg cgcagttcac
    11701 ggatggcgac gatgtcgttt tccggtgcac taccgccgtc aatcaccacc cagttacctt
    11761 tcggcgcggc ggcgcacttt tccgccagca gcgggcctgc gccatcatcg cccatcatgc
    11821 tattgccaac acagagtaaa acgtcagtca cgcagtctcc tcaccatcaa atagatggcg
    11881 ttttcctgat gaatatcatg cagcatactt agcagcattg tactccattc ctgctgctgc
    11941 gaagtttgcg ctgcccgggc tttatcgaat gcgttggcca gcatcggcac atggttaatg
    12001 tcgatgacga tctcaccata tttcggcacg ccttccattt tgcggcgggc ttcgctgccc
    12061 gcctccagag tggcaatcca tgccagatat tcatcccacg ggcaggtgag cgccgcttcc
    12121 aggcaatcga taaccccaag gtggtgacca atcgccaggc tgtaatagac cacctgctgc
    12181 gcctcggcgg gcgtggcatc gttctcatca ataaatttac ggctcagttg actgaacacc
    12241 accttttcac tcatcggata cgcgcctctt ctacaacatg attcagatgg ctgacaatct
    12301 cgttcagacg cggatcgttt tccgcttcca gccagcgtgc aacctgttct tcgccctgcc
    12361 ctaactgtgt caggaagtca tcggcaatct gacgaccgta acgataacct gccaggcggc
    12421 gtgcttcgcg atccactttc acacgcagcg gctgcaccat atcgccatgc aggatttccg
    12481 ccggttgttc atcctgctca cccgggccac gggcgtgaat tttctgctcc agcaggccga
    12541 gcgccattgc aaagccgtac agcgtggcgg caggcgttgg cgggcaacca gggatgtaga
    12601 catctaccgg gacgatttta tccgtaccgc cccacacgca gtagagatcg tggaagatcc
    12661 cgccactgtt accgcaggca ccgtaggaga tacagatttt cgggtccggc gcggactgcc
    12721 acgcacgcag cgcaggggat cgcattgcac gggtgaccgc gccggtaaac agtaaaatat
    12781 ccgcatgacg cggtgaagga acgactttaa tgccgaagcg ttctgcatca aacagcggcg
    12841 aaagcgtggc gaaaatttcg atttcgcagc cgttgcagcc gccgcagtcc acgcggtaaa
    12901 cgtaggcaga acgtttgatt tttttcagta acgacgcctt catgctggcg atggattcat
    12961 ccaccgtcat cgggaccgga atgccgttgg cgtcacgggg gcctaataaa ttgctcatca
    13021 gatggcctct ttcatatggc gagtcagttc aatacggtcg gacggcacca ggcatttctg
    13081 gcgcttacat tccgggcaag tctcaaagct ttcgcggtgg ttttccgcgc ggctgtcacc
    13141 attgtgctta agcagcgcaa tggcgtagtc gatctctttc tggacggcga aaggacgatt
    13201 gcagacgcga cagctgcaca gcacaaaacg ggactgttgc aggaaatctt ctttcttcca
    13261 caccgccagt tcgtactctt gcgacagttt gatcgctgcc gtcgggcaga cttcttcgca
    13321 gcgtccacag aagatgcagc gcccgagatt aaactcccac gcaagctctc ctgtggcgag
    13381 gtcagtttca accgttaagg cgtttgacgg gcaggcattg acgcaggccg cgcagccgat
    13441 gcactgctgc gggttctgct ctggcttacc acggaagttt ttatcaaccg caatcggctc
    13501 cagcggataa gacgaggtcg ccgtgccggt tttgatgact tttttgataa aggtaaacat
    13561 ggcgattcct tatttcagcg gcgagttttt acgctcaatg ctgtaacgct cgagttcttt
    13621 gtacggcacc actttgctct ttttcttacg cacatcgacc acggtcatgc ggtcggtaca
    13681 ggagtagcaa gggtccaggc taccgataat cagcggcgca tcggaaacgg tgttaccgcg
    13741 cagcatgtag cgcagggtcg gccagttcgc gtaggtcgcg gcacggcagc gccagcggta
    13801 cagcttctgg ttgtcgccgg tcatgctcca gtggatatcg tcgccgcgcg gcgcttcggc
    13861 aaagcccagc gcgaaacggt gcggaatgta ggtaaagcct tccaccatca gcggaccgcc
    13921 cggcaggtta tccagaccgt agtcgatcat gttcagcgcg gtataaactt cgttgatacg
    13981 cactttcaga cgggaaataa cgtcgcagcc ctgctcgctg tggacttcca ttggcagcag
    14041 gccataaccg acaaacgggt gatcggcgcg agtatcgcgg gcgtgaccgc tggcgcgaac
    14101 catcgggcca acgttactga aatcgcgggc gatttccggg tccagacgac caatgccgac
    14161 ggtgcgctgt tccatgttcg gcgtactcag cagcacatcc accagctcct gcacttcacg
    14221 acgcatctgt tgcgccagct ggcgggtctg gatcatgtca tctttcaaca gatcgcgacg
    14281 aatcccgccg atcaggttca ggccgtaggt tttacgcgca ccggtgagga tctctgccat
    14341 tttcatggag gtttcacgca cgcggaagaa ctgcataaag ccggagtcaa agccggtaaa
    14401 gtggcaggcg agaccgaggt tgagcagatg cgagtgcagg cgttccacct ccagcagaat
    14461 ggcgcggatc atctgcgcac gttctggcac ctgaataccc atcgcgtttt ctaccgacgt
    14521 ggtgtaggcg gtgctgtggg caaagccgca gatcccgcac acacggtcag agaggaaggt
    14581 cacttcgttg taacccatac gggtttccgc cagtttttcc atgccgcgat gaacgtagaa
    14641 cagacggtag tcggcgtcga taatgttttc gccatcgacg aacagacgga agtggcccgg
    14701 ttcgtcagaa gtgacgtgca gcggaccaat cggcacgacg ttgtttttct tgtcgcccag
    14761 ttcgttgatg aactcgtagg tttcagcatc ggtggtcggc gccggacgct gacgataatc
    14821 catgctgtct ttacgcagcg gataaagttc atccggccag tcatccggca gcaccagacg
    14881 gcgttcatcc ggcagaccaa ccggaatcaa accgtacata tcgcgcactt cgcgctcgcc
    14941 ccacacagcc gccggaacgc gcggcgtcac ggacggatac tccggtttgt tggcgtcaac
    15001 ttcgacgcga accgttaccc aacacttagt gcccttctcc atcgacagca cgtaataaac
    15061 ggcgtaatga ccattcagtt tgcgttcgtc gttaccaaac agcaccgaca gccagccacc
    15121 ctgtttgtag taaagaaact ccaccacttc cggcaggtag ttcaccttta cggtgacagt
    15181 cagctgatct ttggtctgcc aggcgtggtc cagcacgacg cccggaaatg cctcattcag
    15241 cgcggcgaga taatgttgac ctaatttttc ttcagacatg ctcaaactct ctttaatcac
    15301 gccgccagca aggagacgaa cgctaaaaat gcaaagccaa acccagccca ggtaatgcgc
    15361 ggagtaatat caagacgcag acgggccatg ctgttttcga acagcgcgat aaccaggacg
    15421 ccgaccacca gtttgacgat ggcaatcacc agcgccagca gcagtccacc ggcggtgaag
    15481 gtttccattt gtccccacgg aataaacacc ccgacgaaca tctgcaacac caccagctgt
    15541 ttcaggctga taccccattt catgacgcca aagccgctac cgctgtattc ggagagcgga
    15601 ccttcctgca actcctgctc ggcttccgcc aggtcgaacg gcagtttgcc catttcgata
    15661 aaagtggcga acgcacaggc acaaagcgcc agcaccagcg ggatgctctg gctcagcggc
    15721 cagtgataaa cggtgtcggt gatgttgctg atgttggtgg aaccggcaac ctgtgcggca
    15781 acccacagac caagcagcag catcggttcg accagcacgc caagcatcgc ttcacggctc
    15841 gcgccgatag cagtaaacgg gctaccggta tccagaccgg aaatggcaaa gaagaaacgc
    15901 gcgatggcaa agagatacag taaggtgatc aaatcgccca gttgcggcag cggagaaccg
    15961 acggtcacca ccggcagcgc agtagcgata gtcagcatga cgcccaccat cacatacggc
    16021 gtcaggcgga acacccagcc ggaggcatcc gggccaacgc tctggcggcc cagcagtttg
    16081 ataatgtcac gatactcctg caacacaccc ggcccacgac ggttatgtaa gcgggctcgc
    16141 gccacgcggg taataccgga gagcagcggc gcaacggcaa atagcaccag cgcctgaatt
    16201 aacggatata aaacactcat tctcaggctc ctcgtgaaac aataatcacc accagcaccg
    16261 ccagttcaac cagcgccatc cggcggaaca gcaacgcact cccctcgcac tgccagcccg
    16321 gcaccagaga caccggattc agccatttgc gtagtttcag caccggcgca aacgcctgtt
    16381 tcaccggcat ggcaaaaccg tgagcggtaa tcaccattga tttttcgtgg tcgtagccgc
    16441 acacccaggc cgagccgcgg gaacgcgacg gcaaacgatc gcctttgcaa atcgccataa
    16501 tgatgaatgg cagcagcggg caggcaatca gcagcaatgt gatcatgggt tgagaaacgg
    16561 tggtgttagc aggctccaga ggcagaggta cagcagcaga gagcatcggc agcagccacg
    16621 gcgcagcaac accgccaatt acgcagcaaa tcgccagtgc cactacgctt acgctcatca
    16681 ggagcggcgc acaggtggcg ttttcggctt ctttggtacg cggcgcgccg aggaacgtga
    16741 cgccatagac tttcgccata cacatcaccg ccagcgcacc ggtaattgcc agacccacag
    16801 cgagcagcgg ccccagcaga cgggcaacaa acgcgccact attgctcagt ttgaagaagg
    16861 attgatagat aacccattcc ccggcaaacc cgttcagcgg cggcagcgca gccattgcca
    16921 ttaacccgac taacatggcg atggagataa ccggcatttt cttgccaata ccaccgagtt
    16981 tctcgatatc gcgatgaccg gtacggaacc agacgctccc cgccccgagg aacagtacgc
    17041 ttttgaacag gctatggtta agcagatggt acagaccacc gaccaggcca agagcaatca
    17101 gcgccggttg ttcgagcgcg atacccgtta cgccagcgcc cagccccagc aggataatgc
    17161 cgatattttc cagggtgtgg taagccagca ggcgctggat gttgtgttcc atcaacgcat
    17221 acagaccacc gacgaacgcg gtgatcatgc cgagcaccag cagcgcgatc ccccaccaca
    17281 gcggcgcgtt accacccagc agcgacaggg ttaaaatgcc cagcaggcca attttcatga
    17341 ctaccgtaga aaacaacgcg gcagctggcg cagaggcgtt agcatgtgcc tgcggcaccc
    17401 agccgtgcag cggaataatc ccggccagca ggccaaagcc aatcacaccg agcagccaga
    17461 tatcggaacc gagcggcagc tgttgcatac gcatatccag caggcgcaga tccagcgtgc
    17521 cataacgctg ccacagcagc cagcaagcaa tcgccagcag cagagtgcca agacgcccca
    17581 gcgcaaacca cagtttgccc tctttgctgt tgctggtaag gaacaccgca cacaaggcca
    17641 tgacttcagc cattaccacg aacatgccga ggttgctggc gatgacggcg cagacggcgg
    17701 cagccatcaa catattgatc tgcaagccgt tgcacttcac ctgcgcgtgg cgatgccagt
    17761 caatgttgta gaggctgaca aacagaccgc acagaccgag cgtaatcagc caaatcgcgt
    17821 taagcggaga gatttgcaca tcgtagctta ccagcgacag cgcaccgctc acgccaaccg
    17881 cgccagtcag tacagtgaag cccgcggctg ccgtatacag actaccaacc gcgccgccaa
    17941 ttccggctat ccagccactt agcgtctttt gaaaagaaaa gagaaatgcc agaacagcgg
    18001 cggcgacaaa ccatgccacg ccgctattga tcagggaaat tgcgctcatt tagcctctcc
    18061 actttgagcc tgctgaaaca aggtgagatc gccaaagtcc gtgttaaagg tcagctcacg
    18121 cttacgtttg ctgacgcggg cgatatcggt gttatccacc agatgcaggg ctttagtcgg
    18181 gcacatccgc acgcaggccg gaccttgttc atcaaaactg caaaggtcac atttgacggc
    18241 gatcgcgcga atacccggca cccagtcaag cagcgtactg atacgcgccg gagcaggcgg
    18301 tgccggtggc gctttcgggg tattggcatt tgcaggaata tccagcggac ggctgccgga
    18361 aaattcaatt gcgccaaacg ggcaggcgat gccgcacagc ttgcagctta cgcacaggct
    18421 ttcattcaac tgcacggccc catcaacgcg ggtgatggcg ttaaccgggc agaccaccgc
    18481 gcagggtgca tcttcacagt ggtgacagag ctgcggcgca gattcttttt catttagcat
    18541 cactctcagg cgcggcattg attgcaggcc gtgctggcga tgcgtctctg aacaggcggc
    18601 ctcacaagtg tggcagccga tacagagcgt ggagtcagca attacaaaac gattcaccag
    18661 gcattcctca ggtgattgtc atttttgacg aaaacatgcc gatgaaatgt cattttcgac
    18721 actcatcgac acgcccatcc ccaaacaggc gtaacgcctg caaaacgggc aaagcctcag
    18781 ctcatgctgc cgggctttgt ccctttacca gttggcttaa attcaccggc acattgtttt
    18841 caatggcggt gtataagctg tcgtaaacgc cagcgatttt ttcgagatag tgttccagaa
    18901 cctgttcgcg atcgcggatg ttgaccttgc catcaatcat gccgtcaatg ctcagttgcg
    18961 cctgcgcaat ccgctgtttg tcttcgccat cttttgtttc gacgtaatac tcgatggtgt
    19021 ggctgttaaa gccatccgcc agggtgacgt aaatacgaaa atgttcaaaa aggacgaaac
    19081 agagttcttt gtccggcgcg cagctcatgg cgtgatgcag gcgcgctttc gataacgtga
    19141 tcccctgaac cagcgaattg tagtagatgt gccactggtc ctgtaggcga cgatgccgct
    19201 gtgcgatgta atcggctttc tcgcttattt cccaaatagt cattgtcagg ttacccgttt
    19261 aacagagatg ccagctttaa gcattttttg tgccaacttt taatttattg ttattaaaga
    19321 gatttttaag ctaaagatga atttcgtcgc cgtgtcgacg tgtcatttag acatcatcga
    19381 cattattcac cgcagggata atcaacactg gcacaattat tgcttgtagc tgacaatagt
    19441 taatgggagg cgatatgcac gaaataaccc tctgccaacg ggcactggaa ttgatcgaac
    19501 agcaggccgc aaaacacggc gcaaaacgcg taactggggt ctggctcaaa attggcgcat
    19561 tttcttgtgt cgaaaccagc tctcttgcct tttgttttga tctggtttgc cgcggcagcg
    19621 tggcggaagg ttgtaaactg cacctcgaag aacaagaggc cgaatgctgg tgtgaaacat
    19681 gccaacagta tgtgacgcta ttgacccagc gcgtccgccg ctgtccacag tgtcatggtg
    19741 acatgctaca gattgtggca gacgacggtt tacagattcg gcggatagaa atagaccagg
    19801 agtgagcgat gtgtacaaca tgcggttgcg gtgaaggcaa cctgtatatc gagggtgatg
    19861 aacataaccc tcattccgcg tttcgtagcg cgccatttgc cccggcggca cgcccgaaga
    19921 tgaaaatcac cggcattaaa gcgcctgaat ttacccccag ccagactgaa gaaggcgacc
    19981 tgcattacgg tcatggcgaa gcgggcactc acgcaccggg catgagccag cgtcggatgc
    20041 tggaagtcga aattgacgtg ctggacaaaa acaaccgtct ggctgaacgc aaccgcgcgc
    20101 gctttgctgc ccgcaagcaa ctggtgctca acctggtttc cagcccaggt tccggtaaaa
    20161 ccaccctgct gacggaaacc ctaatgcgcc tgaaagacag cgttccgtgc gcggttattg
    20221 aaggtgacca gcaaaccgtg aacgatgccg cacgcattcg cgctaccggc acaccagcga
    20281 ttcaggtgaa cacaggtaaa ggctgccatc ttgacgcaca gatgattgcc gacgccgcac
    20341 cgcgtctgcc actggacgat aacggtattc tgtttatcga aaacgttggc aacctcgtat
    20401 gcccggccag cttcgatctc ggtgaaaaac acaaagtggc ggtgctttcc gttaccgaag
    20461 gtgaagacaa accgctgaaa tatccgcata tgtttgccgc cgcctcgctg atgctgctca
    20521 acaaagttga cctgttgccg tatctcaact ttgacgttga gaagtgcatc gcctacgccc
    20581 gcgaagtcaa tccagaaatt gaaatcatcc ttatttccgc caccagcggt gaagggatgg
    20641 accagtggct gaactggctg gagacacagc gatgtgcata ggcgttcccg gccagatccg
    20701 caccattgac ggcaaccagg caaaagtcga cgtctgcggc attcagcgcg atgtcgattt
    20761 aacgttagtc ggcagctgcg atgaaaacgg tcagccgcgc gtgggccagt gggtactggt
    20821 acacgttggc tttgccatga gcgtaattaa tgaagccgaa gcacgcgaca ctctcgacgc
    20881 cttacaaaac atgtttgacg ttgagccgga tgtcggcgcg ctgttgtatg gcgaggaaaa
    20941 ataatgcgtt ttgttgatga atatcgcgcg ccggaacagg tgatgcagtt aattgagcat
    21001 ctgcgcgaac gtgcttcaca tctctcttac accgccgaac gccctctgcg gattatggaa
    21061 gtgtgcggtg gtcataccca cgctattttt aaattcggcc tcgaccagtt actgcctgaa
    21121 aacgttgagt ttatccacgg tccgggttgc ccggtgtgcg tactgccgat gggtagaatc
    21181 gacacctgcg tggagattgc cagccatccg gaagtcatct tctgtacctt tggcgacgcc
    21241 atgcgcgtac cagggaaaca gggatcgctg ttgcaggcaa aagcacgcgg tgccgatgtg
    21301 cgcatcgttt actcgccgat ggatgcgttg aaactggcgc aggagaatcc aacccgcaaa
    21361 gtggtgttct tcggcttagg ttttgaaacc accatgccga ccaccgccat cactctgcaa
    21421 caggcgaaag cgcgtgatgt gcagaatttt tacttcttct gccagcacat tacgcttatc
    21481 ccgacgctgc gcagtttgct ggaacagccg gataacggta tcgacgcgtt cctcgcgccg
    21541 ggccacgtta gtatggttat cggcactgat gcctataatt ttatcgccag cgattttcat
    21601 cgtccgctgg tggtcgctgg tttcgaaccc cttgatctac tgcaaggcgt ggtcatgctg
    21661 gtggagcaga aaatagcggc ccacagcaag gtagagaatc agtatcgtcg ggtggtaccg
    21721 gatgccggta acctgctggc gcaacaggcg attgccgatg tgttctgtgt caacggcgac
    21781 agcgaatggc gcggcttagg cgtgattgaa tcttctggcg tgcacctgac gccggattat
    21841 caacgattcg atgccgaagc acatttccgc ccggcaccgc agcaggtctg cgatgacccg
    21901 cgcgcgcgtt gtggtgaggt attaacgggc aaatgtaagc cgcatcaatg cccgctgttt
    21961 ggtaacacct gtaatcctca aaccgcattt ggtgcactga tggtctcctc cgaaggagcg
    22021 tgcgccgcgt ggtatcagta tcgtcagcag gagaatgaag cgtgaataat atccaactcg
    22081 cccacggcag cggcggccag gcgatgcagc aattaatcaa cagcctgttt atggaagcct
    22141 ttgccaaccc gtggctggca gagcaggaag accaggcacg tcttgatctg gcgcagctgg
    22201 tagcagaagg cgatcgtctg gcgttctcca ccgacagtta cgtcattgac ccgctgttct
    22261 ttcctggcgg taatatcggc aagctggcga tttgcggcac cgcgaatgac gttgcggtca
    22321 gtggcgctat tccgcgctat ctctcctgtg gctttatcct cgaagaagga ttgccgatgg
    22381 agacactgaa agccgtagta accagcatgg cagaaaccgc ccgcacggca ggcattgcca
    22441 tcgttactgg cgatactaaa gtggtgcagc gcggcgcggc agataaactg tttatcaaca
    22501 ccgctggcat gggcgcaatt ccggcgaata ttcattgggg cgcacagacg ctaaccgcag
    22561 gcgatatgtt gctggtgagc ggcacgctcg gcgaccacgg ggcgactatc cttaacctgc
    22621 gtgagcagct ggggctggat ggcgaactgg tcagcgactg cgcggtgctg acgccgctta
    22681 ttcagacgct gcgtgacatt cccggcgtga aagcgctgcg tgatgccacc cgtggtggtg
    22741 taaacgcggt ggttcatgag ttcgcggcag cctgcggttg tggtattgaa atttcagaag
    22801 cggcgctgcc ggttaaacct gccgtgcgcg gcgtttgcga actgctggga ctggacgccc
    22861 tgaactttgc caacgaaggc aaactggtga tcgccgttga acgcaacgcg gcagagcaag
    22921 tgctggcagc gttacattcc catccactgg ggaaagatgc ggcgctgatt ggtgaagtgg
    22981 tggaacgtaa aggtgttcgt cttgccggtc tgtatggtgt gaaacgaacc ctcgatttac
    23041 cacacgccga accgcttccg cgtatatgct aataaaattc taaatctcct atagttagtc
    23101 aatgaccttt tgcaccgctt tgcggtgctt tcctggaaga acaaaatgtc atatacaccg
    23161 atgagtgatc tcggacagca agggttgttc gacatcactc ggacactatt gcagcagccc
    23221 gatctggcct cgctgtgtga ggctctttcg caactggtaa agcgttctgc gctcgccgac
    23281 aacgcggcta ttgtgttgtg gcaagcgcag actcaacgtg cgtcttatta cgcgtcgcgt
    23341 gaaaaagaca cccccattaa atatgaagac gaaactgttc tggcacacgg tccggtacgc
    23401 agcattttgt cgcgccctga tacgctgcat tgcagttacg aagaattttg tgaaacctgg
    23461 ccgcagctgg ccgcaggtgg gctataccca aaatttggtc actattgcct gatgccactg
    23521 gcggcggaag ggcatatttt tggtggctgt gaatttattc gttatgacga tcgcccctgg
    23581 agcgaaaaag agttcaatcg tctgcaaaca tttacgcaga tcgtttctgt cgtcaccgaa
    23641 caaatccaga gccgcgtcgt taacaatgtc gactatgagt tgttatgccg ggaacgcgat
    23701 aacttccgca tcctggtcgc catcaccaac gcggtgcttt cccgcctgga tatggacgaa
    23761 ctggtcagcg aagtcgccaa agaaatccat tactatttcg acattgacga tatcagtatc
    23821 gtcttacgca gccaccgtaa aaacaaactc aacatctact ccactcacta tcttgataaa
    23881 cagcatcccg cccacgaaca gagcgaagtc gatgaagccg gaaccctcac cgaacgcgtg
    23941 ttcaaaagta aagagatgct gctgatcaat ctccacgagc gggacgattt agccccctat
    24001 gaacgcatgt tgttcgacac ctggggcaac cagattcaaa ccttgtgcct gttaccgctg
    24061 atgtctggcg acaccatgct gggcgtgctg aaactggcgc aatgcgaaga gaaagtgttt
    24121 accactacca atctgaattt actgcgccag attgccgaac gtgtggcaat cgctgtcgat
    24181 aacgccctcg cctatcagga aatccatcgt ctgaaagaac ggctggttga tgaaaacctc
    24241 gccctgaccg agcagctcaa caatgttgat agtgaatttg gcgagattat tggccgcagc
    24301 gaagccatgt acagcgtgct taaacaagtt gaaatggtgg cgcaaagtga cagtaccgtg
    24361 ctgatcctcg gtgaaactgg cacgggtaaa gagctgattg cccgtgcgat ccataatctc
    24421 agtgggcgta ataatcgccg catggtcaaa atgaactgcg cggcgatgcc tgccggattg
    24481 ctggaaagcg atctgtttgg tcatgagcgt ggggctttta ccggtgccag cgcccagcgc
    24541 attggtcgtt ttgaactggc ggataaaagc tccctgttcc tcgacgaagt gggcgatatg
    24601 ccactggagt tacagccgaa gttgctgcgt gtattgcagg aacaggagtt tgaacgtctc
    24661 ggcagcaaca aaatcattca gacggacgtg cgtctaatcg ccgcgactaa ccgcgatctg
    24721 aaaaaaatgg tcgccgaccg tgagttccgt agcgatctct attaccgcct gaacgtattc
    24781 ccgattcacc tgccgccact acgcgagcgt ccggaagata ttccgctgct ggcgaaagcc
    24841 tttaccttca aaattgcccg tcgtctgggg cgcaatatcg acagcattcc tgccgagacg
    24901 ctgcgcacct tgagcaacat ggagtggccg ggtaacgtac gcgaactgga aaacgtcatt
    24961 gagcgcgcgg tattgctaac acgcggtaac gtgctgcagc tgtcattgcc agatattgtt
    25021 ttaccggaac ctgaaacgcc gcctgccgca acggttgtcg cccaggaggg cgaagatgaa
    25081 tatcagttga ttgttcgcgt gctgaaagaa accaacggcg tggttgccgg gcctaaaggc
    25141 gctgcgcaac gtctggggct gaaacgcacg accctgctgt cacggatgaa gcggctggga
    25201 attgataaat cggcattgat ttaactgcaa attgccggac agatctgcct gtccggcata
    25261 ctattcacga ggttttttcg gacgatattt ttccggcagt tctggcaccg gacgcttgtc
    25321 atcgatgaga tgacgcacgg ttaagatcgg atgacgccac agcattctcg gcccggccca
    25381 acgcataatc tgtttcatct cttcacgctt tgcaggctgg taacagtgca ccggacactg
    25441 cttacaggct ggtttctctt cgccgaacac acatttatcc agccgctttt gcgcgtaaac
    25501 aaacaacgcc tcgtaatgct ccggctccgc tgacgcctgc gggcatttcg cttgataaag
    25561 atcgatcatt tttttaatcg tcagtttttc acgagagata cgcttgccgg acatgctgcc
    25621 tccacctcat taagatgtat ttatattaca tcttaatctt aaagggcact atgactctaa
    25681 agaagaaagg ttcgccaacc gacgcaattt tgcgtacttg cttcataagc atcacgcaaa
    25741 agctgcaaaa cagcatcttt cccggaacca gcatcaagaa ctcaccgttc gcttcttccc
    25801 ctgaaatgat taactccggt atcatgtgcg ccttatgtga ttacaacgaa aataaaaacc
    25861 atcacacccc atttaatatc agggaaccgg acataacccc atgagtgcaa tagaaaattt
    25921 cgacgcccat acgcccatga tgcagcagta tctcaggctg aaagcccagc atcccgagat
    25981 cctgctgttt taccggatgg gtgattttta tgaactgttt tatgacgacg caaaacgcgc
    26041 gtcgcaactg ctggatattt cactgaccaa acgcggtgct tcggcgggag agccgatccc
    26101 gatggcgggg attccctacc atgcggtgga aaactatctc gccaaactgg tgaatcaggg
    26161 agagtccgtt gccatctgcg aacaaattgg cgatccggcg accagcaaag gtccggttga
    26221 gcgcaaagtt gtgcgtatcg ttacgccagg caccatcagc gatgaagccc tgttacagga
    26281 gcgtcaggac aacctgctgg cggctatctg gcaggacagc aaaggtttcg gctacgcgac
    26341 gctggatatc agctccgggc gttttcgcct gagcgaaccg gccgaccgcg aaacgatggc
    26401 ggcagagctg caacgcacta atccggcgga actgctgtat gcagaagatt tcgccgagat
    26461 gtcgctgatt gaaggccgtc gcggcctgcg ccgtcgcccg ctgtgggagt ttgaaatcga
    26521 caccgcgcgc cagcagttga atctgcaatt tggcacccgc gatctggtcg gttttggcgt
    26581 cgagaacgcg ccgcgcggac tttgtgctgc cggttgtctg ttgcagtatg cgaaagatac
    26641 ccaacgcacg accctgccgc atattcgttc tatcaccatg gaacgtgagc aggacagcat
    26701 cattatggat gccgcgacac gtcgtaacct ggaaattact cagaacctgg cgggcggtgc
    26761 ggaaaatacg ctggcttctg tgctcgactg caccgtcacg ccgatgggta gtcgtatgct
    26821 gaaacgctgg ctgcatatgc cagtgcgcga tacccgcgtg ttgcttgagc gccagcaaac
    26881 tattggcgca ttgcaggatt tcaccgccga gttgcagccg gtactgcgtc aggtcggcga
    26941 cctggaacgt attctggcac gtctggcttt acgaactgct cgcccacgcg atctggcccg
    27001 tatgcgccac gctttccagc aactgccgga gctgcgtgcg cagttagaaa ctgtcgatag
    27061 tgcaccggta caggcgctac gtgagaagat gggcgagttt gccgagctgc gcgatctgct
    27121 ggagcgagca atcatcgaca caccgccggt gctggtacgc gacggtggtg ttatcgcatc
    27181 gggctataac gaagagctgg atgagtggcg cgcgctggct gacggcgcga ccgattatct
    27241 ggagcgtctg gaagtccgcg agcgtgaacg taccggcctg gacacgctga aagttggctt
    27301 taatgcggtg cacggctact acattcaaat cagccgtggg caaagccatc tggcacccat
    27361 caactacatg cgtcgccaga cgctgaaaaa cgccgagcgc tacatcattc cagagctaaa
    27421 agagtacgaa gataaagttc tcacctcaaa aggcaaagca ctggcactgg aaaaacagct
    27481 ttatgaagag ctgttcgacc tgctgttgcc gcatctggaa gcgttgcaac agagcgcgag
    27541 cgcgctggcg gaactcgacg tgctggttaa cctggcggaa cgggcctata ccctgaacta
    27601 cacctgcccg accttcattg ataaaccggg cattcgcatt accgaaggtc gccatccggt
    27661 agttgaacaa gtactgaatg agccatttat cgctaacccg ctgaatctgt cgccgcagcg
    27721 ccgtatgttg attattaccg gcccgaacat gggcggtaaa agtacctata tgcgccagac
    27781 cgcactgatt gcgctgatgg cctacatcgg cagctatgta ccggcacaaa aagtcgagat
    27841 tggacctatc gatcgcatct ttacccgcgt aggcgcggca gatgacctgg cttccgggcg
    27901 ctcaaccttt atggtggaga tgactgaaac cgccaatatt ttacataacg ccaccgaata
    27961 cagtctggtg ttaatggatg agatcgggcg tggaacgtcc acctacgatg gtctgtcgct
    28021 ggcgtgggcg tgcgcagaaa atctggcgaa taagattaag gcattgacgc tgtttgccac
    28081 ccactatttc gagctgaccc agctaccgga gaaaatggaa ggcgtcgcca acgtacatct
    28141 cgatgcgctg gagcacggcg acactatcgc ctttatgcac agcgtgcagg acggcgcggc
    28201 gagcaaaagc tacggtctgg cggttgcagc tctggcgggc gtaccgaaag aggttattaa
    28261 gcgcgcacgg caaaaactgc gtgagctgga aagcatttcg ccgaacgccg ccgcgacgca
    28321 agtggatggt acgcagatgt ctttgctgtc agtgccagaa gaaacttcgc ctgcggtcga
    28381 agcactggaa aatctcgacc cggattcact gactccgcgt caggcgctgg agtggattta
    28441 tcgcttgaag agtctggtgt aataacaatt cccgatagtc ttttgctatc gggaatatta
    28501 acgacaactg acgaatcaaa taaaaatacc ctgtataata ggaaagctta ttttacaggg
    28561 taaaaccatg ccatctacac gctatcaaaa aatcaatgcc catcactatc gccatatatg
    28621 ggtcgttggt gatattcatg gtgaatatca gttattacaa tcccgtttac atcaactctc
    28681 tttttaccct gaaaccgact tacttatttc cacaggcgat aatattgatc gtgggccgaa
    28741 gagtcttaac gtgctgcgcc tgctaaacca accctggttt acgtcggtta aaggcaacca
    28801 cgaagcgatg gcgttggatg cattcgaaac tggcgatgga aatatgtggc ttgccagcgg
    28861 tggtgactgg tttttcgatt taaatgattc agagcaacaa gaggcaatag atctgttgct
    28921 gaaattccat caccttccac atattattga aatcactaac gacaacataa aatatgtcat
    28981 cgcacatgca gattatccgg ggagtgaata tctctttggt aaagaaatag cggagagcga
    29041 attactctgg cctgttgatc gtgtgcagaa atcgcttaat ggcgagttac aacaaataaa
    29101 cggcgctgat tattttatat ttggacatat gatgtttgat aacattcaga cgttcgctaa
    29161 ccagatttat attgataccg gcacgccgaa aagcgggcgg ctgtcatttt ataaaataaa
    29221 gtagtctcat gcttcttctg tgaagcatga gtaaccaaat gttattgcag gccattattc
    29281 atttttcgca accagcaaag agagatcctg cttcgccagc gcgagactgg cactctccgg
    29341 caaaccgtcg tctgtgataa tctgatcaaa ctcgcttaat ggtaacgcca gccatgtcgc
    29401 cacctgacca tatttcgtcg catcacagac caaaactcgc tggcggctgg cactggcaat
    29461 cgcccgtttc accgtgactt tatcttccgc tggcgtagaa atcccccgca cactccatga
    29521 cgatgcagaa ataaaagcct gatcaatcat caggctgcgc agcatggtcg cagcggcttc
    29581 cccgacacag gaacggtttt cccgacacac tgcaccgcca gtgtgaataa ttgtgcaatt
    29641 actgttgtcg agcaagtagt tcgcaataac gaaatcgttt gtgaccacag tcagtgactc
    29701 catgtgaatc agatgctgtg ctatcgctaa cgtggtcgtt cccgcatcca gatagataca
    29761 acttcccggc tgaacaagac ttgccgccag cttgccaata gccgcttttt gcgtcattgc
    29821 cagcgcagtt tttacctgat gagaaggttc atgcgccacg cgtcccggag actggacgcc
    29881 tccggacacc agcacaacgg ctccctgctg ctccagtttt tgtaaatccc gacgaatggt
    29941 catatgtgac acattcattc tgtccgttag ttcagcaata ctgacaatgc ctttttcagc
    30001 taccatctca aggatgattt ggcgacgctc tacgggtatc aacttttgct ccttcctttg
    30061 tcctgctgac attctacgct atttgcctgc gaaacgtgcg cggcgcaact aacgcttagt
    30121 tcacataaaa taacacacaa tgttaattta tgtgaatcag ctcaccatat cgttatcttc
    30181 cagcgcttat attcacaata tcaaacaaaa tatcacttaa attaacaagg agagcagatg
    30241 aaaacgggat ctgagtttca tgtcggtatc gttggcttag ggtcaatggg aatgggagca
    30301 gcactgtcat gtgtccgcgc aggtctttct acctggggcg cagacctgaa cagcaatgcc
    30361 tgcgctacgt tgaaagaggc aggtgcttgc ggggtttctg ataacgccgc gacgtttgcc
    30421 gaaaaactgg acgctctgct ggtgctggtg gtcaatgcgg cccaggttaa acaggtgctg
    30481 tttggtgaaa caggcgttgc acaacatctg aaacccggta cggcagtaat ggtttcttcc
    30541 actatcgcta gtgctgatgc gcaagaaatt gctaccgctc tggctggatt cgatctggaa
    30601 atgctggatg cgccagtttc tggtggtgca gtaaaagccg ctaacggtga aatgactgtc
    30661 atggcctccg gtagcgatat tgcctttgaa cgactggcac ccgtgctgga agccgttgcc
    30721 ggaaaagttt atcgcatagg tgcagaaccg ggactaggtt cgaccgtaaa aattattcac
    30781 cagttgttag cgggcgtaca tattgctgcc ggagccgaag cgatggcact tgcagcccgt
    30841 gcggggatcc cgctggatgt gatgtatgac gtcgtgacca atgccgccgg aaattcctgg
    30901 atgttcgaaa accggatgcg tcatgtggtg gatggcgatt acaccccgca ttcagccgtc
    30961 gatatttttg ttaaggatct tggtctggtt gccgatacag ccaaagccct gcacttcccg
    31021 ctgccattgg cctcaacagc attgaatatg ttcaccagcg ccagtaacgc aggttacggg
    31081 aaagaagacg atagcgcagt tatcaagatt ttctctggca tcactctacc gggagcgaaa
    31141 tcatgatcaa gattggcgtt atcgccgatg attttaccgg cgcgacggat atcgccagtt
    31201 ttctggtgga aaacggtcta ccaacggtac aaattaacgg tgttccaaca ggtaaaatgc
    31261 cggaagcaat cgacgcactg gtgatcagcc tgaaaacgcg ctcctgtccg gtggttgaag
    31321 ccacacagca atcgctggcg gctctgagct ggttgcaaca gcaaggttgc aaacagatct
    31381 atttcaaata ctgctctact ttcgacagta cggcgaaagg taatatcggc ccggttaccg
    31441 atgcattaat ggatgctctc gacacgccgt ttacggtctt ctctccggcc ctgccggtca
    31501 acggacgtac ggtttatcag gggtatttgt tcgtaatgaa tcaactgctg gccgaatccg
    31561 ggatgcgcca tcacccggta aatcccatga ccgacagcta tcttccccgt ctggttgaag
    31621 cgcaatccac agggcgctgc ggcgtcgttt cggcacatgt tttcgaacaa ggtgtggatg
    31681 ccgttcgtca agagctggct cgcttacagc aagagggcta ccgctacgcg gtgcttgatg
    31741 cgctgaccga acaccatctg gaaattcagg gagaagcctt gcgcgatgcc ccactggtaa
    31801 cgggcggttc tggtctggcg attggcctgg cccggcagtg ggcgcaagaa aacggtaacc
    31861 aggctcgcga agcagggcat ccgctcgctg ggcgcggcgt agtgctctcc ggttcatgct
    31921 ctcaaatgac caaccgccag gtggcacatt accgtcaaat tgcaccagcc cgtgaagttg
    31981 atgtggcacg ctgcctctca accgaaactc tggccgctta tgcacacgaa ctggcagagt
    32041 gggttctggg ccaggaaagt gtacttgctc cactggtttt tgccaccgcc agcactgacg
    32101 cattggcagc aattcaacag caatacggtg cacaaaaagc cagtcaggca gtagaaactc
    32161 tattttctca actagcggcg cggttagcag cggaaggcgt gacacgcttt attgtcgcag
    32221 gcggtgagac ctccggcgta gtcacacaga gcctgggcat aaaagggttt catattggcc
    32281 caaccatttc ccccggcgtg ccgtgggtaa acgcactgga taagcctgtc tcactcgccc
    32341 ttaaatctgg caacttcggt gatgaagcct ttttttcacg agcccaaaga gagtttttat
    32401 catgagcgat ttcgcaaaag tagagcagtc tttgcgagag gagatgacgc ggattgccag
    32461 ttcattcttt cagcgcggct atgcaaccgg ttcggctggc aatctgtcgc tgcttttacc
    32521 tgacgggaat ttactggcaa caccgacagg ttcatgcctg ggcaatctcg atccgcagcg
    32581 gctttccaaa gtcgccgcgg atggcgaatg gttaagtggt gacaaaccct cgaaagaggt
    32641 gctctttcat ctggcgctgt atcgcaacaa tccgcgctgt aaagcggtgg tgcatttgca
    32701 cagcacatgg tcgacggcgc tttcctgcct gcaagggctg gacagcagca acgttattcg
    32761 tccgttcaca ccatacgtgg tgatgcggat gggaaatgtc ccgctggtgc cttattaccg
    32821 accgggcgat aaacgcatcg cacaggatct ggcggaactg gcagcagaca atcaggcttt
    32881 tttactggca aatcatggcc cagtggtttg cggtgaaagc ctgcaagaag ccgccaacaa
    32941 tatggaagag ctggaggaaa cggcaaagct gatttttatt ctcggtgacc gcccgatccg
    33001 ttatctgacc gcaggtgaaa ttgcggaatt aaggagttaa tgcaatgcct cgttttgcag
    33061 ctaatttatc catgatgttc accgaagtgc cttttattga acgcttcgcc gcagcgcgaa
    33121 aagccgggtt cgatgctgtg gaatttctgt ttccctatga ctactccacc ctgcaaatcc
    33181 aaaagcaact ggagcaaaac catctgacac tggcgctgtt caataccgct cccggagata
    33241 ttaatgcggg ggaatgggga ttatccgccc ttcccggacg tgagcacgaa gcacacgcag
    33301 acatcgacct ggcactggaa tatgcattgg cgctcaattg tgaacaagtc catgtgatgg
    33361 caggcgtcgt gcccgctggc gaagacgctg aacggtaccg ggcagtattt atcgataatc
    33421 tccgctacgc tgccgaccgc tttgcgctac acggcaagcg aatattagtt gaagcactca
    33481 gccccgacgt taagcctcat tatctcttct ccagccagta tcaggcactg gctattgttg
    33541 aagaggttgc gcgagataac gtgtttattc aactagatac ttttcatgcg caaaaagtag
    33601 atggcaacct gacgcattta atccgcgact acgctgggaa atatgcgcac gtacaaattg
    33661 ccggactacc tgatcgacat gaaccggacg atggagaaat caactatccg tggctgttcc
    33721 gcctgttcga tgaggtggga tatcagggct ggatcggttg tgaatataaa cctcgtggcc
    33781 tcaccgaaga agggcttggc tggtttgacg cctggcgcta attcgttaaa tccctaatta
    33841 caacgtaccc atacatcccc ccatctacgg atggggtagg ttaactataa ctttcagaca
    33901 gggtttccca tgtccacaat tacattgtta tgcattgcgt tagctggcgt aatcatgctg
    33961 ttgctgctgg tcatcaaggc aaaggtacaa ccattcgttg ctctgctcct cgtcagcctg
    34021 ttagtcgcac ttgcggcggg tataccggcg ggcgaagtgg gtaaagtgat gatcgccggg
    34081 atgggcggtg tgcttggttc cgtcactatt attattggtc tgggcgctat gctggggcgt
    34141 atgatcgaac actctggtgg tgcagagtca ctggctaatt atttcagtcg caagttaggt
    34201 gacaaacgaa ctatcgctgc gctgactctg gcagcgttct tcctcggtat tcccgtcttc
    34261 tttgatgtcg gctttattat tcttgcgcca atcatttacg gttttgccaa ggttgccaaa
    34321 atatcgccac tcaaatttgg cctgcctgtc gctggaatca tgctaactgt tcatgtggcg
    34381 gtaccgccgc atccaggccc tgtcgccgca gcggggttac tccacgcaga catcggctgg
    34441 ctaaccatca tcggtattgc gatttctatt cccgtaggga ttgttggcta ctttgcagcg
    34501 aaaataatca atacgcgcca atatgcgatg tcggtggaag tactggaaca gatgcaactg
    34561 gctccggcca gtgaggaagg cgcaacaaaa ttaagcgata aaataaatcc accgggcgtc
    34621 gcgctggtca cctcgctaat tgttattcct atcgcgatta tcatggcggg tacggtttcc
    34681 gcaacactga tgccgccttc gcatcccctg cttggtacgc tacagctgat cggctcacca
    34741 atggtagctc taatgattgc gctggtgctg gcattctggt tattggcttt acgtcgcggc
    34801 tggagcttac aacacaccag cgacattatg ggctcagccc ttcccactgc ggcggtagtg
    34861 attttggtta ctggtgctgg aggggtattt ggcaaagtgc tggtggaatc gggcgtcggc
    34921 aaagcccttg ccaatatgct acaaatgatt gacctgcctc tgttaccagc cgcatttatt
    34981 atttcactgg ctctgcgtgc atcgcagggg tcagcaaccg tagcaatcct gacaaccggc
    35041 gggttactct cagaagcggt gatgggattg aatccgattc agtgcgtatt ggtgacgctg
    35101 gcagcctgct ttggtgggct tggcgcgtca catattaatg actcagggtt ctggattgtg
    35161 accaaatatc tggggttgtc tgtagcagac ggtctgaaaa cctggacagt gttaacgacc
    35221 attctcggtt ttaccggatt cttaattacc tggtgcgtat gggcggtaat ttgaccttaa
    35281 taaaaaggtc cgatgggcat cggacctttt atttaacgct taccttccac cagcagcggc
    35341 gggatgctcg gcaccattgg cgcgtcatca atatctttct gcgtcatgcg gaacgcttcg
    35401 ggataatgtt cgcggctggt acggcgcagc ggttcggtat cgcgccaggt ataaaggcaa
    35461 tgctggcact gatataccgt ccagacatct ttcaccggcg atttcgccat cacttcaatc
    35521 tgttcatcgg cacaacgtgg acaaatcatc ttcttctcct tatttacgtg cagccagcat
    35581 agcggtcagt ttttcagccc aggctttggt ttccggtaaa tccaccaccg gttggctgta
    35641 gtgaccacgg ttgtccgggg cgacaggcgt agtggcgtcg ataatcagct tgtcggtgat
    35701 ccccgcaggg cttgagcctg gatcgagttc cagcacggac atattcggca actgcaccaa
    35761 atcccctgcc gggttcactt tcgaggagag cgcccacatc acctgcggca ggttgaacgg
    35821 gtcaacgtct tcatcgacca taatcaccat cttcacgtag cccagaccat gcggcgtggt
    35881 cattgcgcgc aggcccaccg cgcgggcaaa gccgccgtag cgttttttgg tggagataat
    35941 cgccagcagg ccatgggtgt acatggcgtt taccgcctgc acttcaggga actcggcttt
    36001 cagctgctga tacagcggca cgcaggtggc tggccccatc aggtagtcga tttcggtcca
    36061 cggcataccg aggtacagcg attcgaaaat cggcctggtg cggtaagaga ctttatcgat
    36121 gcgcaccacg gtcatgttac gcccgccgga gtagtgcccg gtaaactcac cgaacggccc
    36181 ttcgatttcg cgtttacggc tttcgatgac cccttcgagg atcacttctg aaccccacgg
    36241 cacatcaaaa ccggtcaacg gggcggtggc gatcgggtac gggctttcac gcagcgcgcc
    36301 tgccatttcg tactcggact gatcatattt cagcggcgtg gcccccatca gcgtgatgat
    36361 cggatcgtta ccgagcgtga tcgcaatcgg cagatcttca ccgcgctctt ctgctttatg
    36421 cagatgcagg gcgatatcgt gcatcggcac cggttgcagg ccgagcttac gcttgccctt
    36481 cacttccatg cggtagatgc cgacgttctg cttgccgaag ttatccgggt cgagcggatc
    36541 gcgggaaacc acgcacgctt tgtcgagata gaaaccgcca tcgccatcgt ttaaacgaaa
    36601 cagcggcagg atatcgaaca ggttgatctc gtcgccatca acggtgttct gcgcccaggc
    36661 tggattggcg cggcgctccg gggcaatcgg gaagttatcc cagcggcgga taaactcatc
    36721 aatctgcttt ttaaccgggg catttggcgg caggccgagg gaaatcgcgt ggttctgcca
    36781 ggaaccgatg gtgttcatcg ccacgcgggc atcggtaaag ccgcgaatat tatcaaacca
    36841 cagcgcgggc gcgccgtcgc cgatacgccc ggtggcgtta gctgctgctg ccagatccgg
    36901 ctcggcgttc acttcttcgc tgattttcag taactggccg tggtcatcaa gcgcctgtaa
    36961 aaagctgcgt aaatcatcaa atgccattat tcattctcct gagaaaaatt ccgggcctgc
    37021 ggcaatcctt gccagcgcct ggcgtgggga tgttcgaggc caaattgatc cagcacgcgg
    37081 gctaccacat ggtggacaat gtcatctacc gtttcgggat ggttataaaa ggcaggcatc
    37141 ggcggcacca tcgccacgcc catgcgtgaa agtgcgagca tattttcgag atggatggtg
    37201 ctaagcggca tttcacgcgg caccagcacc agtttgcggc cttctttgag cacgacgtcc
    37261 gccgcgcgcc ctaccaggcc atcggcgtaa ccagcgcgga taccggcgag cgttttcata
    37321 ctgcacggaa taacgatcat gccgtcggta cgaaaagaac cggatgagat gatcgccgcc
    37381 tgatccgccg ggttatggct gaagtctgcg agggcagcaa catcgcgagc gctgtaaggc
    37441 gtttccagtt caatggtggt tttcgcccac ttcgacatca ccagatgagt ctcgacattc
    37501 ggcatctccc gcagcgcttg cagtaatgcc acaccaagag gcgcaccggt agcccctgtc
    37561 atcccgacga tcagtttcat tgcttatcct cttaattgtt cgtatacgaa cgttattatt
    37621 aacatactcc ttcatttcca cagcggcaag gattgaaaag taacaacatc ttcttcgtca
    37681 ctaaaaaacc gctatgatag cgacagagaa ttagcccgga gtttacatgg cgttacgaaa
    37741 taaagcgttc catcagttac gacagctttt tcagcagcac acggctcgct ggcagcacga
    37801 gttacctgac ctgaccaaac cacagtacgc ggtgatgcgc gccattgctg ataaacctgg
    37861 tatcgaacag gtggcactga tagaagcagc tgtcagcacc aaagcaacgt tagcagaaat
    37921 gttggcaaga atggaaaatc gtggcttagt cagacgtgaa catgaccccg ctgataagcg
    37981 gcgacgcttt gtctggttga ctgccgaagg cgaaaagata cttgctgcgg cgatacccat
    38041 tggtgacagc gtggatgagg aatttttggg gcgtttgagt ggagaagagc aggagctgtt
    38101 tatgcagctg gtgcgcaaga tgatgaacgc atagggggca aatttccgga tgcgacgcta
    38161 tcgcgtctta tccagactac aaatacaccg actcaccaac cgaaagaaaa aaggccagcc
    38221 tcgcttgaga ctggcctttc tgacagatgc ttacttactc gcggaacagc gcttcgatat
    38281 tcagcccctg cgtttgcagg atttcgcgca aacggcgcag gccttcaacc tgaatctggc
    38341 gaacacgttc acgggtgagg ccaatttcac gacctacatc ttccagtgtt gccgcttcgt
    38401 accccagcaa accgaatcga cgtgccagca cttcacgctg tttggcgttc agctcgaaca
    38461 gccatttgac gatgctctgc ttcatatcgt catcttgcgt ggtatcttcc ggaccgttct
    38521 ctttttcatc ggccaggatg tccagcaacg ctttttcgga atcaccaacc agcggggtgt
    38581 ctaccgaggt aatgcgctcg ttaagacgaa gcatacggct gacgtcatca actggcttat
    38641 ccagttgctc tgcgatctct tccgcacttg gttcatggtc cagcttatgg gacaactcac
    38701 gtgcggttcg caggtaaacg ttcagctcct ttacgatgtg aatcggcaaa cgaatagtac
    38761 gggtttggtt cataatcgcc cgttcaatcg tctggcgaat ccaccaggtt gcgtatgttg
    38821 agaagcggaa accacgttcc gggtcaaact tctctaccgc gcggatcagc cccaggttgc
    38881 cctcttcgat aaggtccagc aacgccagac cacgattgcc ataacggcgg gcaattttta
    38941 ccaccagacg caagttactc tcgatcatcc ggcggcgaga ggcgacatct ccacgcagtg
    39001 cgcgacgcgc aaaataaact tcttcttcgg ccgttaacag tggtgaataa ccaatctcac
    39061 caaggtaaag ctgagtcgcg tccaacacac gctgtgtggc tccctgcgat aacagttcct
    39121 cttcggccaa atcgttatca ctgggttcct cttctactaa ggccttttcg tcaaaaacct
    39181 caactccgtt ctcatcaaat tccgcatctt catttaaatc atgaactttc agcgtattct
    39241 gactcataag gtggctccta cccgtgatcc cttgacggaa cattcaagca aaagcctggt
    39301 tccgccgatt tatcgctgcg gcaaataacg cagcgggttt acggatttcc ccttgtaacg
    39361 aatttcaaaa tgcaagcgtg ttgaactggt tccggtgcta cccatggttg ctattttttg
    39421 ccccgccttc acttcttgtt gttcccggac cagcattgtg tcgttatggg cgtaggcact
    39481 caggtaatca tcattatgtt tgatgataat cagattaccg tagccgcgca gcgcgttacc
    39541 agcataaaca acgcggccat ctgcggtcgc gataattgcc tgtcctttgc tgcctgcgat
    39601 atcaatcccc ttgttgcccc cctcagaagc gccaaaggtt tcgatcactt tgccctcagt
    39661 cggccagcgc caggtggaga taggcgtact ggttgatgta ctgctgacag ttggctcggt
    39721 tgtgcttgct gttggtaccg ttacaggcgc tgtgaccgtg gtcgcagctg gcttgttgtt
    39781 cggcaacatt ttgttagcac tctgttcacc cgaagattca gaatacgtaa ttgtcggttg
    39841 cgacgcaaca gcaacggtgg aattttgtgc aggcttgatc acaactcctt gctctgctgc
    39901 gtcggcctgg gtaatggcat ttccgccagt gattggcgta ccggaagcat tacccacctg
    39961 caaggtctga ccaacgttca gcgcgtatgg tgcctgaata ttgttgcgct gagcaaggtc
    40021 acggaaatcg ttgccagtaa tccaggcgat atagaaaagt gtgtcgcctt ttttcacggt
    40081 ataggtactg ccgctataac tgcctttcgg aatgttccca tactgacggt tatagacgat
    40141 gcgtccgttt tccatctgta ccggctgctg agctactggc tgcactggct ggatttgcgg
    40201 ttgttgagta gcctgaattt gtggctgctg taccggctga atttgcggtt gctgggctgt
    40261 agacgtcgtc cccattttcg gcggcggcgt aatcaacata ccagaattgg tatttgcagg
    40321 cgcattgcca ttaacggagc tgaccggtgc cggtggattt gaagtgtcag aacagcctgc
    40381 cagccatagc gaaaccagtg acaaagccgc aatgcggcga acggtgaatt ttgggcttcc
    40441 cgcgctcatt tatcccccag gaaaaattgg ttaataacca gtgacataat taccgtgcaa
    40501 ggcaccctac tgaacactgg aaaagatgtt cacgatacgc tgacctgcgg caaaataacc
    40561 aggaaaaatc caggtatttc ctcacgtttt aagccagctc acccttcact aaagggacaa
    40621 agcgcacggc ctccacggta tcgataataa attcgcctcc ccgacgacgc acccgtttca
    40681 aatactggtg ctcctcccct acgggtaaga cgagaatccc gccttcgtcc agctgcgtca
    40741 ttagcgcagt tggaatttcc ggcggtgccg ccgtaacaat gatagcgtca aacggcgcgc
    40801 gtgcctgcca accttgccat ccatcgccat gacgggttga aacattatgt aaatcaagat
    40861 ttttcaggcg gcgacgcgcc tgccactgca aacctttaat ccgttcgacc gagcaaacat
    40921 gctggacaag atgcgccagg attgccgttt gatatcccga accagtgcca atttccagca
    40981 cccgcgactg cggcgtcagc tcgagtaatt cggtcattcg cgccaccata tacggctgcg
    41041 aaattgtctg cccctgacct atcggcaagg cgatattgtc ccaggctttt tgttcaaacg
    41101 cttcatcaac gaatttttca cgcggcacgg cggcaagtgc attcagcacc tgctcatcct
    41161 gaataccttg cgcacgtaat tgatccagaa gtgcttgtac gcgtctgctt accattgcgt
    41221 gccaactccc acgctgttta accagtctga aaccacatct tgcgcgctat gcgcagttaa
    41281 atccacatgc agcggcgtga tggagacata gccctcatct accgcagcaa aatcggtccc
    41341 cggaccggca tcacatttac cgcccggcgg gccaatccag tacagcgtat tgccgcgcgg
    41401 atcttgctgc gggatcacct gatctgccgg atgtcgtgta ccgcagcgcg tcacgcgaat
    41461 acctttgatt tgatccaagg gtaaatccgg aacgttaata ttaagaatac gcccggtgcg
    41521 cagcggctct ttacacagtg cgcgcaaaat tgaacaggtt accgccgcgg cagtgtcgta
    41581 atgtttatgc ccgtcaagcg agacggcaag cgccggaaaa cctaaatgac ggccttccat
    41641 cgcggcggct accgtaccgg aataaataac atcatccccc agattcggcc cggcgttaat
    41701 tccggacaca acaatgtccg ggcgcggacg catcagagca ttcacgccaa gatagacgca
    41761 atcggtcggg gttcccattt gcacagcaat atcaccattt tcaaaggtaa acgtgcgcag
    41821 ggaggattcc agtgtcagag aatttgaagc gccgctgcgg ttacgatcgg gggcgaccac
    41881 ctgaacgtca gcaaactcac gcaaggcttt cgccagcgtt tgtataccgg gtgcatgtac
    41941 cccgtcatca ttactcagca atatgcgcat aatcacctgt tgtgttgata agttccctga
    42001 caacgctggt tgcaaaacta cccgccggaa gccagaaacg gatctctacg gtgacgtcat
    42061 cccaccaatt ccagcttaat tgttgcggat acagcagcat cgctctgcgc gcggcttcaa
    42121 ctttttcgcg caccagtaaa gcttgtaatt cagtttctgc ggcgacagct gcttgttcga
    42181 atgccagcgc ttcacgctga gttccccatt cgccactgcc cggcaatgcg gcggttatca
    42241 tcagctcttt atcgttgacg cgacgctgta attccgccag ttcttcggtg gttgcgacaa
    42301 accagctacc acgtccggct aattgtagcg catcgccgtc aacaacttga ttaacgtctg
    42361 cttttttgag gcgctcagca acaatctgat taaacaacgc actgcgggct gccgacaacc
    42421 aaaaactccg tttattgcga tcgcgcaccg gagtattggt ttgcgcccag cgctgcgccc
    42481 cctgcaagtt gctaccgcca atcccaaaac gttgggcacc gaagtagttc ggtacacctt
    42541 ttacgcaaat atcgatcaga cgttgttcaa cgtcatcgcg attgctcact tcgcgcagaa
    42601 ccagggtaaa ggcgttacct ttcagcgcgc ctaaacgcag cttgcgcttg tgccgcgcat
    42661 actccagcac ctggcagcct tccagttgaa aggcgctcag atcgggcatt tccttgcctg
    42721 gcacgcgagc gcataaccac tgttccgtaa cagcatgttt gtctttttgc ccagcgaagc
    42781 tgacttcacg ggcatgaatt ttcaggaatt tcgccagtgc atccgccaca aaacgggtat
    42841 tgcagccgtt tttgaggatt ctaaccagaa tatgctcacc ttcaccatca ggctcaaagc
    42901 ccaaatcttc caccaccaca aagtcttccg gattggcttt cagcagcccg gtgccttgcg
    42961 gtttaccgtg gaggtaagtg agattatcaa actcaatcat tttgttgcct taatgagtag
    43021 cgccaccgct tcacaggcaa tcccttcccc acgtccggta aatccaagtt tttccgtagt
    43081 agtggctttc acgttaacat catccatatg gcagccgaga tcttcggcaa taaacacgcg
    43141 catttgtgga atgtgcggca acatcttcgg tgcctgagcg atgatagtga catcgacgtt
    43201 gcccagggta taacccttcg cctgaatacg acgccaggct tcgcgtagca gctcgcggct
    43261 gtcggcacct ttaaatgccg gatcggtatc cgggaacagc ttgccgatat cccccagcgc
    43321 cgccgcgcca agcaatgcat cggtcaacgc atggagcgcc acgtcgccat cagaatgcgc
    43381 cagcaatcct ttttcgtaag gaatgcgtac gccaccaatg ataattgggc cttcaccgcc
    43441 aaaggcatgt acgtcaaaac cgtgtccaat tcgcattatg tattctcctg atggatggtt
    43501 cgggtgaggt aaaactcggc cagtgccaaa tcttccgggc gcgtgacttt aatgttatcc
    43561 gcacggcctt cgaccaactg aggatggaat ccgcaatatt ccagcgccga ggcttcgtcg
    43621 gtaatagtcg cgccttcatt tagagcgcgc gtcagacagt catgtaacag ctcacgaggg
    43681 aaaaattgcg gcgtcagcgc gtgccataag ccgttgcgat caacggtatg agcaatggca
    43741 tttttgcccg gttcggcacg tttcatcgta tcgcgcactg gtgcggctag gatccctccc
    43801 gtgcggctgg tttcgctcaa cgccaacaat cgcgcgaggt catcctgatg cagacaagga
    43861 cgagcggcgt catgcaccaa tacccactgc gcgtcgccag cggctttcaa acctgccagc
    43921 acggaatcgg cacgctcatc accgccatct acaacggtga tttgcggatg attcgccaga
    43981 ggaagttgtg caaaacggct atcgccagga cttatggcaa tgacgacacg tttcacccgg
    44041 ggatgcgcca gcagcgcatg caccgagtgt tcaagaatgg tttgattacc gattgagaga
    44101 tattgcttag gacattccgt ttgcattcga cggccaaatc cggccgccgg aaccacggcg
    44161 caaacatcca aatgagtggt tgccatgtta attcccgggc tgatttatcg attgttttgc
    44221 cccgcagact gtgcgcgctt cgacgcgtca ggcaccagac gataaaaagt ttcgcccggc
    44281 ctggtcatgc tgagttcatt acgcgcacgc tcttcgagcg cctcctggcc gccattgaga
    44341 tcgtcaattt cggcaaaaag ttgatcgttt cgcgctttaa gtttcgcatt tgtagcttgc
    44401 tgtgccgcca catcatcatt gacgcgggta tagtcatgta taccgttctt accgaaccac
    44461 agcgaatact gtagccagac cagaatagcc agcaacagca gcgttagttt acccatcctg
    44521 ccccctgaaa aacggcatca tcatcccatg catccgaaga cgactctaca tcctctgttg
    44581 gggataccgc gacaacgcgg gcaaatgtac cacatttgtc cattgttacg tatacccagg
    44641 gcgtgcagaa cataatctca ttattagtta cggtttgaat tatgaacaga ggagacggga
    44701 aagtacaaat tagcccagta gccacataaa cagtgcgcca aacataatgc ctactgtcac
    44761 cagggtgaaa acaatactgt agcgtagctt tccgtccatc aatgaatgca gcgcaatccc
    44821 caccactacc gcgacgggca tcagcgccag aaagaaaggc caggtgtaga taaagaagaa
    44881 cagcgtgtta gagccataaa tcaacatcgg catcgccagc gcaaataacc aggagataaa
    44941 accgaccacg gcaccaggca gtgaccatgt ggtttcttca tcctcagtaa ggctgtcgtt
    45001 atttgttagt gtaatgttat ggctattacg catatttgat cctgttactt tgacgaaccg
    45061 ggcatggaaa cccggtggtg tctcaggatc tgataatatc gttctgtctc aacagatcta
    45121 ataattgctg taccaaattt gttactaatt gttcaccatt gagatgaatt tctgccgatt
    45181 caggcgcttc gtaaacggaa tctattcccg taaagttgcg cagttcaccg gcacgcgctt
    45241 tcttatataa gccttttgga tcgcgggctt cgcaaatcgc cagcggcgta tcgacaaaca
    45301 cttcgataaa gcgcccttct cctacgcgtt cgcgaaccat ctggcgttcg gcgcggtgtg
    45361 gcgagataaa tgcggtcagc actaccagtc cggcttcaac catcaaattc gccacttcac
    45421 cgacgcgacg gatattctct ttacgatcgg catcgctaaa accgagatcg ctgcataatc
    45481 cgtggcgaac attatcgcca tccagcagat acgtactgac gccgagttta tgtaacgcct
    45541 cctccagcgc cccggcgacc gttgatttac cggacccgga gaggccggta aaccacagca
    45601 ctacaccacg atgaccgtgg tgtagctcgc gttgttgcac agtgaccgga tggctatgcc
    45661 agacgacgtt ttcgtcatgc agcgccatta tttatccccc agcaaatcgc gcgcgcccca
    45721 gtgcggaaag tggcgacgaa ccagagcatt caattccagt tcgaatgcac tgaattcaga
    45781 tggcgcagca gttgcctggc taactggctc gtgcaccata ccggcaccca cggtcacatt
    45841 gctcaggcga tcgataaaaa tcagcccacc cgtcaccgga ttttgttgat aacgatctaa
    45901 caccagcggc tcgtcaaaag tgagatccac gaggccgatc ccattcagtg gcaggttttc
    45961 aacttcacgc tgggtaaggt tattaatatc aacctgatag cgaatgccat caacacgcgc
    46021 gcgcgtcttc ttaccggcaa ttttgatgtc gtaactctgc cctggagaaa gcggctgttc
    46081 cgccatccat accacatcca ccgacgcgct ctgcaccgcc ggtaacgctt cgtctgccgc
    46141 cagcagcaga tcgccacggc tgatgtcgat ctcatccgtc agcaccaggg tgatcgcttc
    46201 tacggcaaag gcttcttcgc gatcaccatc aaaagtcacg atccgcgcga cgtttgattc
    46261 cacaccagaa ggcagcactt ttacacgttg cccgacttcc acgcgaccgg atgccagcgt
    46321 tccggcgtaa ccacgaaaat cgagattcgg gcgattaacg tactgcaccg ggaagcgcat
    46381 tggctgagca tccaccactc gctggatctc cacggtttcc agcacttcga gcagtgtcgg
    46441 accgctgtac cacggcatac tttcactttg cgaagccacg ttgtcgcctt ccagcgcaga
    46501 gagcggcaca aagcgaatat ccagattgcc aggtaactgt tcggcgaaag tcaggtagtc
    46561 tttacgaatg cgggtgaatg tctcttcact gtaatccacc agatccattt tgttgatcgc
    46621 tacgaccaga tgtttgatcc ccagcagcgt ggagataaaa ctgtgacgac gggtttgatc
    46681 gagcacgcct ttacgggcat cgatcagtaa gatcgccagc tcacacgttg aggcgccggt
    46741 cgccatattg cgggtatact gctcgtgccc gggggtgtcg gcgataataa atttacgctt
    46801 ctcggtagag aaatagcggt aggccacatc aatagtgatg ccctgttcgc gctcagcttg
    46861 caggccgtcc accagcagag ccagatccag tttttcgccc tgggtgccgt gacgcttact
    46921 gtcgttatgc agcgatgaga gctgatcttc gtagatttgg cgggtatcgt gcagcaggcg
    46981 accaatcagg gtacttttgc cgtcatcgac gctaccacag gtcagaaaac gcagcaggct
    47041 tttatgttgt tgcgcaatca tccaggcttc gacgccgcct tcattggcga tttgttgtgc
    47101 aagtgcggtg ttcatcttaa aaatacccct gacgtttttt cagctccata gaccccgcct
    47161 ggtcgcggtc aatcacgcgg ccctgacgtt cactggtggt ggaaaccagc atctcttcga
    47221 tgatctccgg cagtgtttgt gcatttgact ccaccgcacc ggtcagcggc cagcagccca
    47281 gcgtacggaa acgcaccatc cgttttttaa tcacttcgcc cggttgcagg tcgatacggt
    47341 tgtcatcaat catcatcaac ataccgtcgc gttccagaac cggacgttcc gcagcgagat
    47401 acagcggcac aatgtcgata ttttccagcc agatgtattg ccagatatcc tgctcggtcc
    47461 agttagagag cgggaaaacg cggatgcttt cgcctttgtt aatctgcccg ttatagttgt
    47521 gccacagctc cgggcgctga tttttcgggt cccagcgatg gaagcgatca cggaaagagt
    47581 agatacgctc tttagcacgg gatttctctt cgtcgcggcg cgcaccaccg aaggcggcat
    47641 caaaaccgta tttattcagc gcctgcttca ggccttcggt cttcataata tcggtatgtt
    47701 tcgcgctgcc gtgcacgaat ggattaatcc ccatcgccac gccttccggg tttttatgca
    47761 ccagcagctc gcagccgtag gctttcgccg tacgatcgcg gaactcatac atctcgcgga
    47821 atttccagcc ggtatcgaca tgcagcaagg ggaaaggcag cgtacctgga taaaacgcct
    47881 tgcgcgccaa atgcagcatg acgctggaat ctttaccgat agagtagagc atcaccggat
    47941 ttgagaattc tgccgccacc tcgcgaataa tgtggatgct ttccgcctcc agttgccgca
    48001 ggtgagtaag tcgtttttga tccataaccg ttcctttgca ataccgctat tttcttgcca
    48061 tcagatgttt cgactatagg gagcgtaaga gaacgaatga aattaccaat tagaatgagt
    48121 agttccttaa cggaataacg atttggcaaa gctaatatca aaaagtgctt aaggcaccgg
    48181 atttcgggcg tttaggaaga tttgaaattg ttttagcgca gcggcagttt catactatgg
    48241 cggtaaaaaa atttgcatgg tatttaagga ctcactatgt tttccgcatt gcgccaccgt
    48301 accgctgccc tggcgctcgg cgtatgcttt attctccccg tacacgcctc gtcacctaaa
    48361 cctggcgatt ttgctaatac ccaggcacga catattgcta ctttctttcc gggacgcatg
    48421 accggaactc cagcagaaat gttatctgcc gattatattc gccaacagtt tcagcaaatg
    48481 ggttatcgca gtgatattcg gacatttaat agtcggtata tttataccgc ccgcgataat
    48541 cgcaagaact ggcataacgt gacaggaagt acggtgattg ccgctcatga aggcaaagcg
    48601 ccgcagcaga tcatcattat ggcgcatctg gatacttacg ccccgctgag cgatgctgac
    48661 gccgatgcca atctcggcgg gctgacgtta caaggaatgg atgataacgc cgcaggttta
    48721 ggtgtcatgc tggaattggc agaacgcctg aaaaatacgc ctaccgagta tggtattcga
    48781 tttgtggcga ccagcggcga agaggaaggg aaattaggcg ctgagaattt actcaagcgg
    48841 atgagtgaca ccgaaaagaa aaatacgctg ctggtgatta atctcgataa cttaattgtt
    48901 ggcgataaat tgtatttcaa cagcggtgta aaaacccctg aagcagtaag gaaattaacg
    48961 cgcgacaggg cgctggcaat tgcgcgcagt cacggaatag ccgcaacgac caatccgggt
    49021 ttgaataaaa attatccgaa aggcactggg tgttgtaatg acgcagaaat attcgacaaa
    49081 gcgggcattg ctgtactttc ggtggaagcg actaactgga atcttgggaa taaggatggt
    49141 tatcagcaac gcgcaaaaac agccgcattc cctgcgggaa atagctggca tgacgtaaga
    49201 ctggataatc aacagcatat tgataaggca cttcctggaa gaatagaacg tcgctgccgt
    49261 gacgttatgc ggataatgct accgttggtg aaggagctgg cgaaggcgtc ttgatgggtt
    49321 tgaaaatggg agctgggagt tctaccgcag gggcggggaa ttctaagtga tatccatcat
    49381 cgcatccagt gcgcccggtt tatccccgct ggcgcgggga acacaaaacc aaacttctcc
    49441 ataaattcca tagccgcggt ttatccccgc tggcgcgggg aacacacgcg cgtaccggat
    49501 cgcggacaac aaattgccgg tttatccccg ctggcgcggg gaacacgcag tggtgaggcc
    49561 gggaagcgcg gttgagtccg gtttatcccc gctggcgcgg ggaacacggc aacataacga
    49621 acaaaatcaa cgtcaacctc ggtttatccc cgctggcgcg gggaacactt gctgaaaaag
    49681 aaggctccgg cgttatcagt cggtttatcc ccgctggcgc ggggaacaca cggtccatgg
    49741 cacacaggat ccagacgtta acggtttatc cccgctggcg cggggaacac actaagcata
    49801 cataactgtt tttaaacaaa tttattccgc atcaacaatc taccaactaa attaaaaca
//
LOCUS       DAZGVQ010000015        49660 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000015 DAZGVQ010000000
VERSION     DAZGVQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.015"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(62..1306)
                     /gene="mtr"
                     /locus_tag="ACHLAG_000842"
     CDS             complement(62..1306)
                     /gene="mtr"
                     /locus_tag="ACHLAG_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFP0654123.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(1460..3349)
                     /gene="deaD"
                     /locus_tag="ACHLAG_000843"
     CDS             complement(1460..3349)
                     /gene="deaD"
                     /locus_tag="ACHLAG_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFP0654124.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(3342..3422)
                     /gene="yrbN"
                     /locus_tag="ACHLAG_000844"
     CDS             complement(3342..3422)
                     /gene="yrbN"
                     /locus_tag="ACHLAG_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFP0654125.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(3529..4413)
                     /gene="nlpI"
                     /locus_tag="ACHLAG_000845"
     CDS             complement(3529..4413)
                     /gene="nlpI"
                     /locus_tag="ACHLAG_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFP0654126.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(4522..6657)
                     /gene="pnp"
                     /locus_tag="ACHLAG_000846"
     CDS             complement(4522..6657)
                     /gene="pnp"
                     /locus_tag="ACHLAG_000846"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFP0654127.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(6904..7173)
                     /gene="rpsO"
                     /locus_tag="ACHLAG_000847"
     CDS             complement(6904..7173)
                     /gene="rpsO"
                     /locus_tag="ACHLAG_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFP0654128.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(7322..8266)
                     /gene="truB"
                     /locus_tag="ACHLAG_000848"
     CDS             complement(7322..8266)
                     /gene="truB"
                     /locus_tag="ACHLAG_000848"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFP0654129.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(8266..8667)
                     /gene="rbfA"
                     /locus_tag="ACHLAG_000849"
     CDS             complement(8266..8667)
                     /gene="rbfA"
                     /locus_tag="ACHLAG_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFP0654130.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(8831..11503)
                     /gene="infB"
                     /locus_tag="ACHLAG_000850"
     CDS             complement(8831..11503)
                     /gene="infB"
                     /locus_tag="ACHLAG_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFP0654131.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(11528..13015)
                     /gene="nusA"
                     /locus_tag="ACHLAG_000851"
     CDS             complement(11528..13015)
                     /gene="nusA"
                     /locus_tag="ACHLAG_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFP0654132.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(13043..13495)
                     /gene="rimP"
                     /locus_tag="ACHLAG_000852"
     CDS             complement(13043..13495)
                     /gene="rimP"
                     /locus_tag="ACHLAG_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFP0654133.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(13702..13778)
                     /locus_tag="ACHLAG_000853"
     tRNA            complement(13702..13778)
                     /locus_tag="ACHLAG_000853"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13742..13744),aa:Met,seq:cat)
     gene            14126..15469
                     /gene="argG"
                     /locus_tag="ACHLAG_000854"
     CDS             14126..15469
                     /gene="argG"
                     /locus_tag="ACHLAG_000854"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFP0654134.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(15477..17101)
                     /gene="yhbX"
                     /locus_tag="ACHLAG_000855"
                     /pseudo
     CDS             complement(15477..17101)
                     /gene="yhbX"
                     /locus_tag="ACHLAG_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(17560..17646)
                     /locus_tag="ACHLAG_000856"
     tRNA            complement(17560..17646)
                     /locus_tag="ACHLAG_000856"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17610..17612),aa:Leu,seq:gag)
     gene            complement(17661..17993)
                     /gene="secG"
                     /locus_tag="ACHLAG_000857"
     CDS             complement(17661..17993)
                     /gene="secG"
                     /locus_tag="ACHLAG_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFP0654135.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(18221..19558)
                     /gene="glmM"
                     /locus_tag="ACHLAG_000858"
     CDS             complement(18221..19558)
                     /gene="glmM"
                     /locus_tag="ACHLAG_000858"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFP0654136.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(19551..20399)
                     /gene="folP"
                     /locus_tag="ACHLAG_000859"
     CDS             complement(19551..20399)
                     /gene="folP"
                     /locus_tag="ACHLAG_000859"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFP0654137.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(20489..22423)
                     /gene="ftsH"
                     /locus_tag="ACHLAG_000860"
     CDS             complement(20489..22423)
                     /gene="ftsH"
                     /locus_tag="ACHLAG_000860"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFP0654138.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(22523..23152)
                     /gene="rlmE"
                     /locus_tag="ACHLAG_000861"
     CDS             complement(22523..23152)
                     /gene="rlmE"
                     /locus_tag="ACHLAG_000861"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFP0654139.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            23278..23571
                     /gene="yhbY"
                     /locus_tag="ACHLAG_000862"
     CDS             23278..23571
                     /gene="yhbY"
                     /locus_tag="ACHLAG_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFP0654140.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(23727..24203)
                     /gene="greA"
                     /locus_tag="ACHLAG_000863"
     CDS             complement(23727..24203)
                     /gene="greA"
                     /locus_tag="ACHLAG_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFP0654141.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            24451..25884
                     /gene="dacB"
                     /locus_tag="ACHLAG_000864"
     CDS             24451..25884
                     /gene="dacB"
                     /locus_tag="ACHLAG_000864"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP0654142.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(25924..27096)
                     /gene="cgtA"
                     /locus_tag="ACHLAG_000865"
     CDS             complement(25924..27096)
                     /gene="cgtA"
                     /locus_tag="ACHLAG_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFP0654143.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(27112..28077)
                     /gene="yhbE"
                     /locus_tag="ACHLAG_000866"
     CDS             complement(27112..28077)
                     /gene="yhbE"
                     /locus_tag="ACHLAG_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP0654144.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(28204..28461)
                     /gene="rpmA"
                     /locus_tag="ACHLAG_000867"
     CDS             complement(28204..28461)
                     /gene="rpmA"
                     /locus_tag="ACHLAG_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFP0654145.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(28482..28793)
                     /gene="rplU"
                     /locus_tag="ACHLAG_000868"
     CDS             complement(28482..28793)
                     /gene="rplU"
                     /locus_tag="ACHLAG_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFP0654146.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            29052..30023
                     /gene="ispB"
                     /locus_tag="ACHLAG_000869"
     CDS             29052..30023
                     /gene="ispB"
                     /locus_tag="ACHLAG_000869"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFP0654147.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            30250..30528
                     /gene="sfsB"
                     /locus_tag="ACHLAG_000870"
     CDS             30250..30528
                     /gene="sfsB"
                     /locus_tag="ACHLAG_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFP0654148.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(30576..31835)
                     /gene="murA"
                     /locus_tag="ACHLAG_000871"
     CDS             complement(30576..31835)
                     /gene="murA"
                     /locus_tag="ACHLAG_000871"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP0654149.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(31890..32144)
                     /gene="ibaG"
                     /locus_tag="ACHLAG_000872"
     CDS             complement(31890..32144)
                     /gene="ibaG"
                     /locus_tag="ACHLAG_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFP0654150.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(32304..32597)
                     /gene="mlaB"
                     /locus_tag="ACHLAG_000873"
     CDS             complement(32304..32597)
                     /gene="mlaB"
                     /locus_tag="ACHLAG_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFP0654151.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(32597..33232)
                     /gene="mlaC"
                     /locus_tag="ACHLAG_000874"
     CDS             complement(32597..33232)
                     /gene="mlaC"
                     /locus_tag="ACHLAG_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFP0654152.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(33251..33802)
                     /gene="mlaD"
                     /locus_tag="ACHLAG_000875"
     CDS             complement(33251..33802)
                     /gene="mlaD"
                     /locus_tag="ACHLAG_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFP0654153.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(33807..34589)
                     /gene="mlaE"
                     /locus_tag="ACHLAG_000876"
     CDS             complement(33807..34589)
                     /gene="mlaE"
                     /locus_tag="ACHLAG_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFP0654154.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(34597..35406)
                     /gene="mlaF"
                     /locus_tag="ACHLAG_000877"
     CDS             complement(34597..35406)
                     /gene="mlaF"
                     /locus_tag="ACHLAG_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFP0654155.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            35616..36593
                     /gene="yrbG"
                     /locus_tag="ACHLAG_000878"
     CDS             35616..36593
                     /gene="yrbG"
                     /locus_tag="ACHLAG_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFP0654156.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            36607..37593
                     /gene="kdsD"
                     /locus_tag="ACHLAG_000879"
     CDS             36607..37593
                     /gene="kdsD"
                     /locus_tag="ACHLAG_000879"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFP0654157.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            37614..38180
                     /gene="kdsC"
                     /locus_tag="ACHLAG_000880"
     CDS             37614..38180
                     /gene="kdsC"
                     /locus_tag="ACHLAG_000880"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFP0654158.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLHAQGKLDEAKGQSI"
     gene            38177..38752
                     /gene="lptC"
                     /locus_tag="ACHLAG_000881"
     CDS             38177..38752
                     /gene="lptC"
                     /locus_tag="ACHLAG_000881"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFP0654159.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            38721..39278
                     /gene="lptA"
                     /locus_tag="ACHLAG_000882"
     CDS             38721..39278
                     /gene="lptA"
                     /locus_tag="ACHLAG_000882"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFP0654160.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            39285..40010
                     /gene="lptB"
                     /locus_tag="ACHLAG_000883"
     CDS             39285..40010
                     /gene="lptB"
                     /locus_tag="ACHLAG_000883"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFP0654161.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            40058..41491
                     /gene="rpoN"
                     /locus_tag="ACHLAG_000884"
     CDS             40058..41491
                     /gene="rpoN"
                     /locus_tag="ACHLAG_000884"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFP0654162.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            41514..41801
                     /gene="hpf"
                     /locus_tag="ACHLAG_000885"
     CDS             41514..41801
                     /gene="hpf"
                     /locus_tag="ACHLAG_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFP0654163.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            41919..42410
                     /gene="ptsN"
                     /locus_tag="ACHLAG_000886"
     CDS             41919..42410
                     /gene="ptsN"
                     /locus_tag="ACHLAG_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFP0654164.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            42456..43310
                     /gene="rapZ"
                     /locus_tag="ACHLAG_000887"
     CDS             42456..43310
                     /gene="rapZ"
                     /locus_tag="ACHLAG_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFP0654165.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            43307..43579
                     /gene="npr"
                     /locus_tag="ACHLAG_000888"
     CDS             43307..43579
                     /gene="npr"
                     /locus_tag="ACHLAG_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFP0654166.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            43792..44424
                     /gene="yrbL"
                     /locus_tag="ACHLAG_000889"
     CDS             43792..44424
                     /gene="yrbL"
                     /locus_tag="ACHLAG_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFP0654167.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSGEA"
     gene            complement(44421..45149)
                     /gene="mtgA"
                     /locus_tag="ACHLAG_000890"
     CDS             complement(44421..45149)
                     /gene="mtgA"
                     /locus_tag="ACHLAG_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFP0654168.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(45146..45799)
                     /gene="elbB"
                     /locus_tag="ACHLAG_000891"
     CDS             complement(45146..45799)
                     /gene="elbB"
                     /locus_tag="ACHLAG_000891"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFP0654169.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(46029..48365)
                     /gene="arcB"
                     /locus_tag="ACHLAG_000892"
     CDS             complement(46029..48365)
                     /gene="arcB"
                     /locus_tag="ACHLAG_000892"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFP0654170.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(48461..49390)
                     /gene="yhcC"
                     /locus_tag="ACHLAG_000893"
     CDS             complement(48461..49390)
                     /gene="yhcC"
                     /locus_tag="ACHLAG_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFP0654171.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
BASE COUNT        12218 a        13463 c        11986 g        11993 t
ORIGIN      
        1 gataagcgaa gcgcatcagg cattgtgtag gcagcagaaa tgtcggataa ggcaccgctg
       61 attactgata caccggcagt aaattaaagc tcgataaaat atgcaccagt gcgttgccga
      121 cgccaaacac cagaatcagc gcaatcatcg gcttgccacc ccagacgcgg aatttcgggc
      181 tgccaaagcg tttacgcgat gcgcgggcta acagcgccgg aacaattgcc gcccagatgg
      241 tagctgctaa accagcataa ccaatggcgt acaggaatcc gttcgggaac agtagaccac
      301 ccacaacagg cggggcaaag gtcagcaatg ccgttttcaa gcgccccagg gctgagtcat
      361 cgaaaccaaa cagatctgcc agatagtcaa acaaacccag cgttacgcca aggaacgaac
      421 tcgctaccgc aaagtttgag aacacgacca gcagaaggtc caggctacgg ctgttcagca
      481 cgccgcttaa tgcctgtacc agcacatcaa tattaccgcc cttctctgca ataccgataa
      541 actccggacg cgggatgtta cccatcgtcg ccagcaacca gatggtatac agcgccagtg
      601 ccatcagcgt accgtacacc aggcatttca cgatggtttt cggatctttg ccgtaatact
      661 tcatcaggct tggcacgtta ccgtgataac caaacgacgc caggcagaac ggcaatgtca
      721 tcagcagata cggcgcataa gatgcattgc tttctgcaac gttgaacaat gtcgcaggct
      781 gcacatgccc caacaggctg ccaaaggtga ggaagaaggt aatgactttc gcccccagca
      841 caatcgccgt catgcggctg acggccttag tactcaacca caccacaaac gctaccagca
      901 gtgcaaaacc aaaacccgcc gcccgtgccg ggacgtttag cgacatctct gcgaaggtgt
      961 gatgcagaat tgaaccactg gcagaaatat aggcataggt caggatatag agcacaaagg
     1021 caatggaaat accgttgacc acgttccagc ctttgcccag caaatctttg gtgatggtgt
     1081 caaaactcga accgattcgg taattcaggt tagcttccag aatcatcaag ccggaatgca
     1141 gcatacagaa ccaggtaaag atcagcgccg ccattgacca gaaaaaccac gccccggaca
     1201 tgaccactgg cagagaaaac atccctgcgc caataatggt gccgccgata atcaccacgc
     1261 cgccaagcag cgacggtgac gtttgggtgg tggttagtgt tgccatgagg gcttctctcc
     1321 agtgaaaaat agtgcgactg cgttgttatg cattgcactg taccagtaca cgagtacaaa
     1381 agacagaaaa aaagccccga tggtaaaaat cggggctgta tatattattt tacagattgt
     1441 gttcgctgtt cagcgatgat tacgcatcac caccgaaacg acgacgaccg gtagagtcat
     1501 cacgacgcgg agcgcggcct tcacgacgtt cgccgctaaa acgacgacca tcaccacggc
     1561 caccttcacg gcgttcaccg ctgaagttac gaccgccttc acgacgttcg ccaccgaaac
     1621 cacgaccacc gccacgacgc tcaccgccag tatgcggctg tgcatcgccc agtaactgca
     1681 tgttcatcgg cttgttgaga atgcgagtgc gcgtaaagtg ttgcagcact tcacccggca
     1741 tacctttcgg cagttcgatg gtggagtgag aagcaaacag cttgatgtta ccaatgtaac
     1801 ggctgctgat gtcgccttcg ttagcaatcg caccaacgat atgacgaact tcaacaccat
     1861 catcgcggcc cacttcaatg cggtacagct gcatatcgcc aacatcacga cgttcacgac
     1921 gcggacgatc ttcacggtca ccacgcgggc cacggtcgtt acgatcgcgc ggaccacggt
     1981 catcacggtc acggaattca cgtttcggac gcatcggcgc atctggcggt acgatcagag
     2041 tacgttcacc ctgtgccatt ttcagcagtg ccgcagccag agtttcgaga tccagctctt
     2101 caccttctgc agtcggctga attttgctca gcagtgcgcg gtattgatcc agatcgctgc
     2161 tttccagctg ctgctgtact ttagcggcga atttttccag acggcgtttg cccagcagtt
     2221 ctgcgttcgg cagttctact tccggaatag tcagcttcat agtacgttca atgttgcgca
     2281 gcagacgacg ctcgcggttc tcaacgaaca gcagcgcgcg gccagcacga cccgcacgac
     2341 cggtacgacc gatacggtga acgtaagact cagaatccat cgggatatcg tagttaacta
     2401 ccaggctgat acgctcaacg tccaggccac gggctgcaac gtcggtcgca atcaggatgt
     2461 ccagacgacc atctttcagg cgttccagtg tctgctcacg cagcgcctgg ttcatgtcac
     2521 cgttcagcgc ggcactgttg tagccgttac gctcaagagc ttcagccact tccagagtcg
     2581 catttttggt acgaacgaag ataatcgccg catcaaaatc ttccgcttcc aggaaacgta
     2641 ccagtgcttc gtttttacgc ataccccaga cagtccagta gctctggctg atgtcaggac
     2701 gggtagtcac gctggactga atgcgcactt cctgcggctc tttcataaag cggcgggtaa
     2761 tgcgacgaat cgcttccggc atggttgcag agaacagagc ggtctgatga ccttccggga
     2821 tctgcgccat aatggtttca acgtcttcga tgaagcccat gcgcagcatt tcgtcagctt
     2881 catccagaac cagaccgctc agtttagaga ggtccagagt gccacgtttc aggtggtcca
     2941 gcagacggcc cggagtaccg acaacgatct gcggcccctg acgcagggcg cgtaattgca
     3001 cgtcataacg ctggccgccg tatagagcaa ccacatttac gccgcgcatg tgtttagaga
     3061 aatccgtcat tgcttcagca acctgtaccg ccagttcgcg ggtcggtgcc agcaccagaa
     3121 tctgtggtgc tttcagctca ggatcaagat tctgcaacag aggtaaagag aatgctgcag
     3181 tttttccgct ccccgtctgg gccataccca gaacgtcgcg gccattcagc agatgtggaa
     3241 tacactctgc ctgaattgga gatggttttt cgtaacccag atcgttaagg gcttcaagga
     3301 taggagcctt caggcccaga tctgcaaaag tggtttcgaa ttcagccatg tagtacgtgt
     3361 gcctcaaaat taatggcggc cagtctacat aactcatcat gaaattgatc agcaattttc
     3421 attgaaaagt gtgaaccggc tcaaagtagg tgtattaacg aacaacaacg ccctcacccg
     3481 ttaaggtgat ggcaatcaaa aaagattacg ggctgatgtg tacgtcagct attgctggtc
     3541 cgattctgcc aggtcatctt ggtcctggcc caggagcgat aattccaaca atgcgtatcg
     3601 gtgctcaaca aagttatgaa cgttgttggc aaccgccagt ttgaacagtg ccgtggcgct
     3661 gtccaaatcc cccagactta ggtagtactt acctaaatag aagttggttt cactgagatg
     3721 ctcagcgagc gaggtgttat ccgttgcgtc cgccttgagc ctttccatta acgtttgttc
     3781 gctaatgttg cccaggtaga actcgacaat gttccatccc cactgttcct tatccgattt
     3841 ttcgaagtgc tgtttcaaca cttctttagc ctgcttctca tcgagcttct gctcggcgag
     3901 ataaagccac agactacgga aaggatcatt gggatcgtct tgataaaacg ccagcagatc
     3961 atcttgcgct aacttgtcac gaccgccgta atataatgcg atcccgcgat tcaagtgcgc
     4021 gtagttgtaa gttggatcaa gctcaagtac agaatcaaac gcttcatagg cagcatcaaa
     4081 attgcctgcc tgcgttaaat atatgcctaa gtaattgaat acttcaggca tatccggtcg
     4141 gattgccagc gcttgcgaaa aatcgttacg cgctaatgcc ctcagaccga gactatcata
     4201 caacactccg cgctcatata aaagctgtgc gcgttcgtca tcggttaaag cccgactggc
     4261 aaggatttgt tccatacgtg ccagaatcac ttcctgctgt aaagtcggtt gcaatggtac
     4321 cgcgaggact tcacttttac gccaggaagt attactgcat cctgcaagcg taagtgctgt
     4381 cgcaacgaaa caccagcgca aaaaaggctt catttcccac tcccgaagac cacggttgaa
     4441 tgaacgtcct gttcccggtt gctaacaagg cgtcctgccc ggttaaaagc cccccgccgc
     4501 agcggagggc aaatggcaac cttactcgcc ctgttcagca gccggagctt ccggtgctgc
     4561 agcaggttga gactgctcag tcgcttcttt aatgctcaga cggatacggc cctggcgatc
     4621 aacttccaga actttcaccg gtacttcctg acccatctgc aggtaatcgg tcactttctc
     4681 aacgcgtttg tcagcgattt gagagatgtg gaccagacct tctttaccgc cgccgatggc
     4741 aacaaatgcg ccaaagtcaa cgatacgggt cactttacca gtgtagacgc ggcccacttc
     4801 gatttctgca gtgatctctt cgatacgacg aatagcatgt ttcgctttct cgccgtcggt
     4861 cgctgcgatc ttcacagtac cgtcatcttc gatttcgatg gtagtgccag tttcttcggt
     4921 cagggcacgg ataacagagc cgcctttacc gataacgtct ttgatcttat ccgggttgat
     4981 cttgatggta tggatacgcg gtgcgaactc agagatatcg ccacgcggcg cgttgatcgc
     5041 ctgttccatt acgcccagga tatgcagacg cgcaccttta gcctggttca gcgcaacctg
     5101 catgatctct ttggtgatac cttcaatttt gatatccatc tgcagtgcag agataccgtc
     5161 gcgggaacct gcaactttga agtccatatc gcccaggtga tcttcgtcgc ccaaaatgtc
     5221 agacagtaca acgtagttgt cgccttcttt caccagaccc attgcgatac ccgcaacggc
     5281 agctttgatt ggcacacctg cgtccatcag cgccagagac gcgccgcaca cggaagccat
     5341 agaagaagaa ccgttggatt cggtgatttc agacactaca cgtacggtgt acgggaattt
     5401 gtccatatcc ggcatgactg ccagcacgcc gcgcttcgcc agacgaccgt gaccaatttc
     5461 acgacgcttc ggagaaccga ccatgccggt ttcgcctacg gagtacggag ggaagttgta
     5521 gtggaacagg aaggtatcgg tacgttcgcc catcagttca tcaagaacct gcgcgtcacg
     5581 agcggtaccc agcgttgcag tgaccagtgc ctgcgtttca ccgcgggtga acagcgcaga
     5641 accgtgagta cgcggcagca cgccagtacg cacatccaga ccacggatca tatctttttc
     5701 acgaccgtcg atacgcggtt cgcctgccag tacgcggcta cgaacaacgt ttttctcgat
     5761 agcgtgcaga atttcaccca gttcgttttc gtccagggtt tcgtcttcag caagcagcgt
     5821 cgcgatggtt tcagatttga tgacatcaac ctgcgcataa cgctcttgtt tgtcggtgat
     5881 gcggtaagca tcgctcaggc gagcttcagc cagtgcagca acgcgcgcgt tcagcgcttc
     5941 gtttaccggc tccggctgcc agtcccaacg tggtttaccg gcttctttca ccagttcatt
     6001 gatgttctga ataacaacct gctgttgttc atgaccgaac actactgcgc ccagcatctg
     6061 gtcttcgctc agcagttcag cttcagattc aaccatcaga acagcggctt cagtaccggc
     6121 aacaaccaga tccagtttgc tctctttcag ctcgtcctga gtcgggttca gtacgtactg
     6181 gtcattgatg taacctacgc gagcagcacc aatcggacca ttgaacggaa taccagacag
     6241 agacagtgct gcggaagcac caatcatcgc gacgatatcc gggttaactt gcgggttaac
     6301 agaaaccacg gtggcgataa cctgaacttc gttgacgaag ccttccggga acagcgggcg
     6361 aatcgggcgg tcaatcagac gcgcgatcag ggtttcgcct tcgcttgggc ggccttcacg
     6421 acggaagaag ctacccggga tacgaccagc agcgtaggta cgctcctgat agttaacggt
     6481 cagtgggaag aagtcctgac ctggtttggc ttttttctgg ccaacaacgg taacaaatac
     6541 cgcggtgtca tccatgctaa ccataacagc ggcagtagcc tgacgagcca tcatgccggt
     6601 ttccagagtc acggtgtgtt ggccgtactg gaatttacga acgatcggat taagcaatgt
     6661 aatatccttt ctctttctta gacagtacct tacggcactg gtgttaatac ccgatcttct
     6721 gcgcatcctc gcgactaatg acaaccctaa cccagctcta tgtgggtaaa gcctctcatt
     6781 agccgcgcga acctctgcaa cggaagatca ttcatagcaa caatacatta gtttccagtg
     6841 aattgctgcc gtcagcttga aaaaaggggc cactcaggcc cccttttctg aaactcgcaa
     6901 gaattagcga cgcagaccca ggcgctcgat gagctgggtg taacgtgcta cgtctttacg
     6961 tttcaggtag tcgagcagtt tacgacgctg agaaaccatg cgcagcagac cacgacggct
     7021 gtggtgatct tttttgtgct ctgcaaagtg gccctgcagg tggttgatct gtgcagtcag
     7081 cagtgctacc tgaacttcgg tagaaccggt gtcgtttgcg tcacgaccaa actcagaaac
     7141 gattttagct gttgcttcag tacttagaga cattttaaaa ctccaaagta tatagaatga
     7201 aaggacgccg atctctaatt cagcgatccc agtgtacgtt acgcaaagtt ttaaacaatt
     7261 tacgcgacgt taagcggcag tattctactc gtagcgacct gttatcgcaa gacggttaac
     7321 attacgccgg gtattcaacc accaggcgac gaggcgcaac gcggccttca tcgtcaattt
     7381 cgcccatacc gataaatttg ccgttctcac cttctgtaac gcgaaccagt ccttccagcg
     7441 gcgcaccgga tgtacgaact gggttaccat ttttgaagta aacagaagac gttaacggaa
     7501 gattcaccac cgggtagtcc gaagctggac tgtccattgg catcagtaat ggatcaagta
     7561 actccgcagc cggaatatcc tgctgttcgg cttgttcgac cagttcacgc agatgttcca
     7621 gggtcaccat ccgttcaacc ggatatttac ttaccgccag acgacgcagg taaataacat
     7681 gcgcgccaca accgagtttt tcacccaggt catcaatgat ggtgcggata taagtgcctt
     7741 ttgagcagtg aatttccagc tccagctcat tgccttcgtg gcgaataaac agcaattcat
     7801 aaacggtaat cgggcgcgct tcacgcggta cttcaatgcc ctgacgcgca tattcgtaca
     7861 gttttttacc ctgatatttc agtgctgaat acatcgaagg gatctgttcg atatcgccac
     7921 ggaaagtatc cagcgccgcc gccagttgct ctgcgctaaa ggttaccgga cgttcttcaa
     7981 cgatttgtcc gtcggcatca gaagtatcgg tacgctgtcc aagacgcgca atgacccgat
     8041 agcgtttgtc ggagtccagc agatactggg aaaacttcgt cgcttccccg aggcaaattg
     8101 gcaacatgcc agttgccagc gggtccagcg caccggtatg cccggcacgg ttggcgttat
     8161 aaatccgttt cactttttgc agcgcatcat tgctggacat accctgcggt ttatccagca
     8221 acaaaacgcc gttaatgtcg cgaccgcgac gacgaggacg actcattagt cctccttgct
     8281 gtcgtccggg ttaacacgac gttcttcgtc atgtttgacc acgctggtca ccaggtttga
     8341 catgcgcatc ccttcaacca gagagttgtc gtagaagaag gtcagttccg gcacgatacg
     8401 caggcgcatc gctttcccca gcaggctgcg gatgaaacca gaagcttctt gcaacgcttt
     8461 gatgcccgct ttaaccgcgt cttcatcttt gtcgttgagg aacgtcacat atactttggc
     8521 atacgccagg tcgcgagaca tttcgacacc ggaaacggtg gtcatcatgc ccaggcgagg
     8581 atctttaatt tcacgctgca ggatgagagc gatctctttt tgcatttcct gcgctacgcg
     8641 ctgcgggcga ccaaattctt tcgccataat aaattctcct gacaaaaaag gggctgttag
     8701 ccccttttta aaattaattt caggtggaag ggctgttcac gttggcctga taagacgcgc
     8761 cagcgtcaca tcaggcaacg gctgccggat gcggcgtaaa agccttatcc ggcctacaac
     8821 ccagacaacc ttaagcgatg gtacgttgga tttcgatgat ctcgaatact tcgatcacat
     8881 cgccagtgcg gacatcgttg tagttcttaa cgccgatacc acattccata ccgttacgga
     8941 cttcgttaac gtcatctttg aagcggcgca gggactccag ctcgccttcg tagataacca
     9001 cgttgtcacg cagaacgcgg atcgggttgt gacgtttaac cacaccttcg gtaaccatac
     9061 agcctgcgat ggcaccaaat ttcggcgatt tgaacacgtc acgaacttcc gccagaccga
     9121 taatctgctg tttcagttcc ggagacagca taccgctcat cgccgctttc acttcgtcaa
     9181 tcaggttata gatgacggag tagtaacgca gatccaggct ttccgcttca atcactttac
     9241 gtgcagaggc atcagcacgt acgttgaagc caaccaggat ggcgttggac gccgcagcca
     9301 gggtggcgtc ggtttcggtg ataccaccta cgccagaacc gatgatcttc actttaactt
     9361 cgtcagtaga cagtttcagc aaggagtcgg agatcgcttc cacagaaccc tgtacgtctg
     9421 ccttcaggac gatgttcact tcgtgaactt cgccttcggt catgttggcg aacatgttct
     9481 cgagtttaga tttctgctga cgcgccagtt taacttcgcg gaatttaccc tgacgataca
     9541 gtgcaacttc acgcgctttc ttctcgtcac gtacaacggt aacttcatca cccgcagccg
     9601 gtacgccgga caggccgagg atttccaccg gaatggacgg acccgcttcc agcacttcct
     9661 gacccagttc gttacgcatc gcacgaacac gaccgtattc gaagccacac agaacgatat
     9721 cgcccttgtg cagagtacct tcacgtacca gaacggtagc aaccggacca cgacctttat
     9781 cgaggaagga ttcgataacc gcaccgctcg ccataccttt acgtaccgct ttcagctcca
     9841 gaacttccgc ctgcagcagg atagcgtcca gcagttcatc gatgccggta cccgctttcg
     9901 cagatacgtg tacgaactgg ctttcaccgc cccactcttc cggcaggatg ccatactggg
     9961 agagttcgtt cttaacacga tccggatcgg cttctggttt atcaatcttg ttcactgcaa
    10021 ccaccaccgg tacctgcgcc gctttcgcgt gctggattgc ttcgatggtc tgcggcatca
    10081 cgccgtcgtc ggcagcaaca accaggacta cgatgtccgt tgcctgcgca ccacgtgcac
    10141 gcattgaagt aaacgcggcg tgccccgggg tgtctaggaa ggtgatcatg ccgttttcag
    10201 tttcaacgtg gtatgcacca atgtgctggg taatgccgcc cgcttcgcca gaggcaactt
    10261 tcgttgaacg aatgtagtcc agcagagagg ttttaccgtg gtcaacgtga cccatgatgg
    10321 tcacaaccgg cgcgcgcggt tcagccgcag cacccgtgtc acggtcgctc attaccgcct
    10381 cttccagctc gttttcacga cgcaggataa ctttgtggcc catctcttca gcaaccagct
    10441 gtgcggtttc ctgatcgata acctggttga tggttgccat tgcgcccagt ttcatcatcg
    10501 ctttgatgac ctgagagcct ttaaccgcca tcttgttcgc cagttcgcca acggtgatgg
    10561 tttcgccgat cacaacgtca cggttaacgg cctgagctgg cttctggaag ccttgctgca
    10621 gggaagaacc tttacgtttt ccgcctttac cgccacgtac tgctgcgcgt gcttcttcac
    10681 gatcagcttt tgattcagcg tgtttgttgc ctttcttcgg acgcgctgct ttcgcgttac
    10741 gaccacggcc acgaccgcct tcgacttcac gatcgctttc gtcttctgcc tggcgagcat
    10801 gttgagaagt agtgacgtga taatcgctgg aatcttcagt cggttccgcg ttatcagtcc
    10861 atttgttttc ttccgccata cgacgtgctt cttcagcaac gcgacgtgct tcttcttcga
    10921 gtttacgacg cgcttcttct tcggctttac gcttgagctc tgcagcttcc tgctcacggc
    10981 gggctttttc agcctgggcg tttttagtca tatcgtcttg ttgattgctc actttgtctt
    11041 tttccgcagc ttcacgtttc gcttgttcag cagcttcacg cttagcttgt tctgcagcct
    11101 cacgttcagc tttttgttgc gcctcgcgtt tagccgattc ttctgcctca cgacgggctt
    11161 gctcttccgc ttcacgctgc gcttgctctt ccgctgcaag gcgttcagcc tcttgcggat
    11221 cgcgtttcac aaaggtgcgt ttcttgcgga cttcgatttg taccgatttg ctttttccac
    11281 cggtaccagg aatgttaagg gtgctgcgtg ttttacgttg cagcgtcaat ttgtccgggc
    11341 ctgaattttt ctgattcagg tggtcaatca aagtctgttt ctcttgtgcg gacacagagt
    11401 cgtcagcaga cttccggata cctgcatcag caaattgctg taccaggcgt tccacggagg
    11461 tctgtcgctc tgcggccagc gttttaatcg ttacatctgt catgctgttc cttcctgcta
    11521 cagtttatta cgcttcgtca ccgaaccagc aaatattacg ggcagccata atcagtgctc
    11581 cggctttttc gtcggtcaac ccttcgatat cagccagatc atcaatgccc tgttcggcga
    11641 gatcttccag cgtacaaacg ccacgggcgg ccagtttgaa tgccaaatca cgatctatcc
    11701 cttcaaggtt cagcagatcg tcagccggtt tgttatcacc gaggctttct tcctgggcct
    11761 gtgcaatggt ggccagtgca tttttagcac gctcgcgcag tgcttcaacg gtcggctcat
    11821 caaggccttc gatttccaac agctctttca tcggcacata ggccagttct tccagcgtcg
    11881 agaagccttc ttctaccaga acagtcgcga agtcttcgtc gatgtcgaga tatttggtga
    11941 aggtgtcgat cgctgcgtgc gcttccgcct gatgcttagc ctgcaggtcg tcaacggtca
    12001 tcacgttgag ttcccaaccg ctcagctgcg aagccagacg cacgttctga ccgttacggc
    12061 caatcgcctg cgccaggtta ccggcttcaa cggcgatatc catggtgtgt ttatcttcat
    12121 ccaccacgat agaagcaacg tctgccggtg ccattgcgtt aatcacaaac tgcgccgggt
    12181 tatcatccca caggacgata tcgatacgct cgccgcccag ttcagtagaa accgcctgaa
    12241 cacgcgcgcc acgcatacct acacaggcac ctaccggatc gatacgttta tcgttggttt
    12301 tcaccgcgat tttcgcacga gaacccggat cgcgagccgc tgctttaatt tcaatcactt
    12361 cttcgccgat ttctggcact tcaatacgga acagttcgat cagcatttcc ggcttggaac
    12421 gagtgacgaa cagttgcgcg ccacgcgctt ccgggcgaac ggaatagagc acgccacgaa
    12481 cgcggtcgcc agggcggaag ttttcacgcg gcagcatatc ttcgcgcagg atcacggctt
    12541 cagcattgtt gcccagatcc agagagatgt tgtcgcggtt tacttttttc accacgccgg
    12601 tgatgatttc accttcgtgt tcacggaact gatcaaccac catcgcacgt tcggcttcac
    12661 gcactttctg cacgataacc tgttttgccg tctgggtagt gatacggtca aaggtaacag
    12721 actcaatctg atcttcaacg taatcgccca ggttcaggct ttcatcttca taacgtgcgg
    12781 cttcaagggt gatttccttg gtcggctggg tgacttcatc aacaactaac cagcgacgga
    12841 aggtgtcaaa atcaccgctt ttgcgatcga tctgtacgcg gacgtcgatc tcttgttcat
    12901 attttttctt tgttgctgtc gccagcgcgc tttccaatgc ttcgaaaatc ttctcgcgag
    12961 gtagcgcctt ttcattggat acggcttcaa ctacagccaa aatttctttg ttcatcgcgg
    13021 gcttttcacc tcatccagac tattaaaagt ggggaaccag gttcgccttc tggatattac
    13081 tcagcgcgaa cacttcatct ttaccttcga cggtaactgt gatcatttca ccgtctaccg
    13141 ctttgataac gccctgccat ttacgacggt tttgtaccgc catacggaga accagagtca
    13201 cctcttctcc gacaaaacgg gcgtagtgtt cagccgtgaa cagtgggcga tcgagacccg
    13261 gtgaggagac ttccaggtta taagcaacgg tgatgggatc ttcaacatcc agcacagcac
    13321 ttacctggtg gctcacatca gcacaatcat caacattgat gccatcttca ctatcaatat
    13381 agatgcgcag tgtggatgtg cgaccgcgaa taaattcgat gccaaccagt tcaaaaccca
    13441 gggcctcaac tggcgcagta atcatctctg ttaatttttg ctctaatgtg gacaagccca
    13501 cccccaagac ataaaaaaag ggcctaaagc ccagttattc tgtagtcaga taacaaaaaa
    13561 ccccgataaa tcggggcttt atataactga accctataac cgcaactgcg gtctggagca
    13621 ctttccagaa ggattttttc aaatcccact acgaaggccg aagtcttcac agtatatttg
    13681 aaaaaggact ctaagggaaa gtggttgcgg gggccggatt tgaaccgacg accttcgggt
    13741 tatgagcccg acgagctacc aggctgctcc accccgcgcc tgaaacgtgg caaattctac
    13801 tcgttttggg taaaaaatgc aaatactgct gggatttggt gtaccgagac gggacgtaaa
    13861 atctgcaggc attatagtga tccacgccac attttgtcaa cgtttattgc taatcatgtg
    13921 aatgaatatc cagttcactt tcatttgttg aatacttttg ccttctcctg ctccccctta
    13981 agcgcattat tttacaaaaa acacactaaa ctcttcctgt ctccgataaa agatgattaa
    14041 atgaaaactc atttattttg cataaaaatt cagtgagagc ggaaatccag gctcatcatc
    14101 agttaattaa gcagggtgtt attttatgac gacgattctc aagcatctcc cggtaggtca
    14161 acgtattggt atcgcttttt ccggcggtct ggacaccagt gccgcactgc tgtggatgcg
    14221 acaaaaggga gcggttcctt atgcatatac tgcaaacctg ggccagccag acgaagagga
    14281 ttatgatgcg atccctcgtc gtgccatgga atacggcgcg gagaacgcac gtctgatcga
    14341 ctgccgcaaa caactggtgg ccgaaggtat tgccgctatt cagtgtggcg catttcataa
    14401 caccaccggc ggcctgacct atttcaacac gacgccgctg ggccgcgccg tgactggtac
    14461 catgctggtt gctgcgatga aagaagatgg cgtgaatatc tggggtgacg gtagcaccta
    14521 taaaggaaac gatatcgaac gtttctatcg ttatggtctg ctgaccaatg ctgaactgca
    14581 gatttacaaa ccgtggcttg atactgactt tattgatgaa ctgggcggcc gtcatgagat
    14641 gtctgaattt atgattgcct gcggtttcga ctacaaaatg tctgtcgaaa aagcctactc
    14701 cacagactcc aacatgcttg gtgcaacgca cgaagcgaag gatctggaat acctcaactc
    14761 cagcgtcaaa atcgtcaacc caattatggg cgtgaaattc tgggatgaga gcgtgaagat
    14821 cccggcagaa gaagtcacag tacgctttga gcaaggtcat ccggtagcgc tgaacggtaa
    14881 aacctttagc gacgacgtag aaatgatgct ggaagctaac cgcatcggcg gtcgtcacgg
    14941 cctgggcatg agcgaccaga ttgaaaaccg tatcatcgaa gcgaaaagcc gtggtattta
    15001 cgaggctccg gggatggcac tgctgcacat tgcgtatgaa cgtctgttga ccggtattca
    15061 caacgaagac accattgagc agtatcatgc acatggtcgt cagttgggcc gtctgctgta
    15121 ccaggggcgt tggtttgact cccaggcgct gatgctgcgt gactctctgc aacgctgggt
    15181 tgccagccag atcactggtg aagttaccct ggagctgcgc cgtgggaacg attattcaat
    15241 cctgaatacc gtctcagaga acctgaccta caagccagag cgtctgacga tggaaaaagg
    15301 cgactcggtg ttctcgccag atgatcgtat tggtcaattg accatgcgta atctggatat
    15361 cactgatacc cgagagaaac tttttggtta tgccaaaact ggcctgctct cctcctctgc
    15421 cacttcaggc gtgccgcagg tggagaatat ggaaaacaaa ggccagtaat tcgacatcaa
    15481 ccctgccctt caggggtggg gttaccttgc ttatcctctg taaactcctt tctgagcatc
    15541 acataatcga aaacatcatg gtttgcatcc acaacccgtg agtctccttt atagtgtgca
    15601 atcacttcct caagcgtttg cggctgttcc ggctttgtta cccccatcca cgcattaata
    15661 aggtaattat tgtaagctgt cgaaaagatg ttgttttccg ttgtgcgatc cacgccatcc
    15721 ccaagaacgg ggctatacca gataaacatc gggacatgat atgcctgctg gctagcctcc
    15781 ctgcctccat gaaaatagac gttcttcttc gtagggtcac gttccaggcc gtggtcggca
    15841 aaatacatga ccgaggcgcg gcgatctttt aataattcaa aaacctgacc tagcaaacta
    15901 tctgtgtaat gaatggagtt gtcatagcag gcatcctgat cgtcctgcgg ttgaaacacg
    15961 gcgctggatt gcggataggc gctacaagcc ggttcatggc ttccatttaa atgaagaaca
    16021 atcagtttct tttgctgcgt attttgctgt aatgcttgcg ataaatgcgg caacaacaat
    16081 tcatcaaatc ctctgacata aactgtttcc atggcgcgca tggcgatact ggtaactgct
    16141 gtaccattct gccgaaaagc ggattgcgag cttagccaga aagtctgaaa tcctgcttga
    16201 ttagccatat taataatgtt gtcggggtaa ttatgaatgt catgactcaa aacagaatca
    16261 gcagttaaag aaaggggaac cgacagcgca gtgtaaggtg cgccgcttat tgcttgatta
    16321 aacagtttga tctgttttct ttgtgcttca acttgcggtg tcgtagagcg tgtatatcca
    16381 tacaaagaca tattgtcgac acgtacagac tcccccacaa tcaacacgta ggtatcaata
    16441 cctgtatccc tgactgataa ttgaaaatac ggcaccgtgt ttgcaattga gagtaatctt
    16501 tgatgctctt tcgctgctaa agcaaaatag ttgagattga aaaacggcgt ataggtagca
    16561 aatcgcgacg ctagtatata tggactgaac gcttttttat tttttgcatc tttataagca
    16621 aattgacaag cggaaaataa actgcccgaa atgacaatca gcaataatat tcctgtcact
    16681 tttttgtcgg caaggaaaca tcatatttta ttattactgc caaaaaaaga agggataaaa
    16741 aagcaaaggc acatagataa gggatataca tccccagcat tttgacaact tcatccggat
    16801 cactctggag cacactaatc gcaaatccat cattgaaagt tgtaccaaag gtacaccatg
    16861 aatagaggct aatactcatg tctgccgcag tcagtaacac aaatggtgcg gcaacaagca
    16921 ggcgaagcgg aagggatgat atacgtttta caaccaggat ggtcaggacg aagaacagca
    16981 atcgctgaac catatgcgcg ccggaagagg cgactaagtt cgtaatacca aaaacaataa
    17041 cacaaagata caacaaccaa tgagatttaa aacttctagc aaatttgttg aatactgtca
    17101 tccagaaatc caagttcaca accaatataa acaattatca acaatgttca attttatatc
    17161 agacatgttt ttcaactctg atccatatca gaccgaaaca ctgttagttt tttcttttta
    17221 aatgatgcgg ccattgcgtt ctcagtaatt aaataacatt atcaatgcgt ctctaaacat
    17281 tacgcagaac gtagccatca gtagttatac tgacaggagc aggatggttt atcgagaagt
    17341 aaacatatcc gtattcgtac cggaactgga gttgacacaa taaagtgcca attatgtcag
    17401 cagaagggaa aagtttataa ctaaggcgta ccggcaccat cgtttcaagg taccagctac
    17461 gagtaaagca actggacgag atacagatac ctgacaacca ttcctcagac caggaccaaa
    17521 acgaaaaaag acgcttttca gcgtctcttt tctggaattt ggtaccgagg acgggacttg
    17581 aacccgtaag ccctattggg cactaccacc tcaaggtagc gtgtctacca attccaccac
    17641 ctcggcacgg atactacttt ttagttcggg atatcgctgg tcggcttagc cggagcagct
    17701 ggttgagttt gttcggtttt caccggtgca ctcagatttt cccattcgct acctttattg
    17761 gttttgttgc tattgatgtt acccagcacc agactgatga tgaagaataa cgttgccagc
    17821 agcgccgtca tgcgggtcat gaagttacca gaaccacttg aaccaaacag cgtagcggaa
    17881 gcgcctgctc cgaaggaggc tcccatatca gcgcctttac cttgctgcag catgatcaga
    17941 ccaacaaggc caattgccac aataaggaaa actactaaaa gagcttcgta cataatcaac
    18001 ctgttccttg cggagttgcc gcgtaccaat gcttcaacca ataaagcggg agtttttaat
    18061 ctttcccact gaagcgggtg tgaatactaa ccaaagcgaa tgaccttcgc aagggcaatt
    18121 ttatcgcatt gtatcaactg cggaaaaaaa cagcaaaacc gatgtgttcg ctgagaaaaa
    18181 aggcgacaat cgccgccttt ttagccagtt atctaacgct ttaaacggct tttactgcat
    18241 cggcgatgcg gtgtgcaaat tcagtcacct gcgcttcgtc ttcgccttcc accatcacgc
    18301 gaattaacgg ttcggtgccg gatttacgca gcaacacgcg tccacggttg cccagcgcag
    18361 cttcaacctc tgcggtcacg gctttaactg actcatgctc aagtggatcg ccgctacctg
    18421 cggtgtaacg tacgttaacc agaatctgcg ggaacatttt catgccgctg caaaggtcgt
    18481 gcaggctcat atggttacgt gccatcgccg ccagcacctg caagccagca acgatgccgt
    18541 caccggtagt agttttatcc agtaggatca catgaccgga attctctgca ccgatacgcc
    18601 agcctttctc ctgcattttt tccagtacgt agcggtcacc cactttcgcg cgcgcaaatg
    18661 gaattcctaa ctgtttcagc gccagttcaa gccccatgtt gctcatcaat gtacccacag
    18721 cgccaccacg cagctggccc tgacgaagac cttcacgcgc gatgatatac atgatctgat
    18781 cgccatcgac tttattgcct tcatggtcga ccataatcac gcgatcgcca tcgccgtcga
    18841 aggcaatacc gagatccgct ttttcagcca gcacacgagc ctggagcgcg cgaacgtcgg
    18901 tagcccccac ttcggcattg atgtttacac cgtttggctc acaaccgata gcgataacgt
    18961 tcgcccccag ttcgcgcagc acgttcggcg cgatgtgata agtcgcaccg tttgcacaat
    19021 ccaccacaat cttcagttca ctgaggctaa gttcgttcgg gaacgtggct ttgcaaaact
    19081 cgatatagcg acccgcggca tcaacgatac ggctggcttt acccagttct gccgaatcaa
    19141 cgcagctgat ctccttttcc atttccgctt cgatggcctc ttctaccgca tccggcagtt
    19201 tggtgccgtc gatagagaag aatttaatcc cattatcgta gaacgggtta tgcgatgcag
    19261 atatcacaat tccggcctct gcgcggaagg tacgcgtcag ataagccacg gccggtgttg
    19321 gcatcgggcc agtgaagagt gcggaaaggc ccgctgccgc cagacccgct tccagtgctg
    19381 actccagcat atagccagaa atacgcgtgt ctttaccaat aataatctta cgggagccgt
    19441 ggcgcgccag cactttaccc gcggcccaac ccagcttaag cacaaaatca ggtgtgatcg
    19501 gcgcatcccc tacacgacca cgaatcccat cggtaccgaa atatttacga ttactcatag
    19561 cgtttgtttt cctttgcaga cagagtggct tccaccaccc gcatcgcttc tacggtttct
    19621 ttgacgtcat gaacacgaat gatgtgcgcg ccttgcattg cggcaatgac cgcacaggcc
    19681 agactaccgc tcaggcgctc ggacggcccc acgttcagca gctgcccaat catcgatttt
    19741 cgtgacatac ccaccaacag cggcaggttg aaatggtgaa attcagccag gcgcgccagt
    19801 aatgaatagt tatgggagag atttttaccg aaaccgaatc cggggtcgag caacaatttc
    19861 tcttttgcga tacccgcctg ctcgcaacgt gctatttgct caataaagta gcgattcact
    19921 tctgcaaaga catcgtcata cttcggagct tcctgcatgg tttttggatt tccctgcata
    19981 tgcatcagac aaaccggtaa accggtttct gcagccgcct ccagagcgcc aggttcggaa
    20041 agggagcgga tatcattaat aatgtgagcg ccaactttcg ctgactcacg gatgacttct
    20101 ggtttggatg tatcgaccga gatccagact tcgaagcgtt gagcaattgc ctcaaccaca
    20161 ggaataacac gttgcaactc ttcttcaacg ctaacttccg ccgcccctgg gcgcgtggac
    20221 tcgccaccaa cgtcaatgat cgtcgcgcca gcattgatca tcagattcgc atgtttcacc
    20281 gcatctatca gcgagttatg cgtgccgcca tccgaaaagg aatcaggcgt gacgttgagg
    20341 atccccatta cgtgaggatg gctaaggtcc agtgaagtac cctgggcaaa gagtttcatg
    20401 atgttatccc tggtatgaat tgataagaaa aaaccccgga gcacgccccg gggttttcgg
    20461 tacaaataca gtcatctgat gcgggaactt acttgtcgcc taactgctct gacatggtgt
    20521 tacccgggtt cggcgtacgc ggttcatcaa ccggacgagg agcctttgga ctaccattgt
    20581 cgccagaatt gttagaagcg cctggttctt cccagcccgc tggcggacgt acatcgcgac
    20641 gtgccatcag gtcatcaatc tgcggtgcgt cgatagtctc atatttcatg agagcatctt
    20701 tcatcgcatg cagaatatcc atattgtcgg tcagaagctg acgcgcacga ttatagttac
    20761 gctcaatcag tgctttcact tcctggtcga tgatacgtgc agtttcatcg gacatatgtt
    20821 tcgctttcgc tacgctacgg ccgaggaata cttcaccttc ttcttccgcg tacagcagtg
    20881 gacccaattt ctcagagaag ccccactggg tcaccatgtt acgtgccaga ttggtcgcaa
    20941 ctttaatatc gttggacgca ccggtagaaa catgttccgg cccatagatg atctcttctg
    21001 ccagacgacc accgtacagc gtagaaatct ggctttccag tttctgacgg ctggcgctga
    21061 ttgcgtcgcc ctcaggcaag aagaaagtca cacccagcgc acgaccgcgt gggataatcg
    21121 tcactttgtg caccggatcg tgttccggca ccaggcgacc gataatcgca tgacccgctt
    21181 cgtggtaagc cgttgattct ttctgcgctt ccgtcatcac catggagcga cgttccgcac
    21241 ccatcatgat tttgtctttc gctttctcga actcaaccat cgacacaacg cgtttgttgc
    21301 cacgagcagc gaacagtgcc gcttcgttca ccaggttcgc caggtcagca ccggagaaac
    21361 caggagtacc acgggcaatg attgccgcgt cgatatcggg ggccaatggt acgcgacgca
    21421 tgtgaacttt caggatctgc tcacgaccgc gaacatctgg caagccgacc acaacctgac
    21481 ggtcgaaacg gccaggacgc agcagggccg ggtcgagaac gtccggacgg ttagtcgcgg
    21541 cgataacgat gataccttcg ttaccttcga agccatccat ctcaaccagc atctggttca
    21601 gagtctgttc acgttcatcg tgaccaccgc ccagaccagc gccacgctgg cggcctacgg
    21661 cgtcgatttc atcgataaag atgatgcacg gtgccgcttt cttcgcctgt tcgaacatgt
    21721 cacgaacacg ggatgcaccc acaccgacga acatttctac gaagtcagaa ccggagatag
    21781 taaagaacgg aactttcgct tcgcctgcaa tcgctttcgc cagcaacgtt ttaccggtac
    21841 ccggaggacc gaccatcagg acgcctttcg ggatcttacc gccgagtttc tggaagcggc
    21901 tcggctcgcg cagatactct accagttcag caacttcttc ttttgcttcg tcgcagcccg
    21961 caacgtcagc aaaggtcgtt ttgatctgat cttccgtcag catgcgcgct ttgctcttac
    22021 caaacgacat ggcacctttg ccaccgccgc cctgcatttg acgcatgaag aagatccaga
    22081 caccaatcag caacagcatc gggaaccagg agatgaagat agaagccagc aggcttggtt
    22141 cttcaggcgg ttcaccgaca accttgacgt tcttggtcaa caggttatcc agtaatttcg
    22201 gatcctgaac cggaatgtaa gtggtataac ggttactatc tttcttggta acgttgattt
    22261 cacgtccgtt gatacgcgct tcacgaacct ggtcgttatt gacctcttgt aggaaggtag
    22321 agtaatccac cttacggcca ttagactcgc tgggcccaaa gctctggaat actgacatca
    22381 gcacaacggc aatgaccagc cagagtatta ggtttttcgc catgtcactc aagggattaa
    22441 cctcttatta caactgtgtt aaaaacagcg tcaggatact ctatatccag catctttcaa
    22501 actttcgtct gaaatctccc ggttagggtt tacgcccggt cgctacaata tacacttccc
    22561 gcgaacgtgc acgagaagag tccggcttac gaactttgac cttcgtaaac agggagcgaa
    22621 tttcccttag atactcatcg aaaccttcgc cctggaacac cttcactaca aaactgccac
    22681 ctggcgctaa tacatcacga cacatttcta gcgccagttc caccagatac atggcacggg
    22741 ggatatccac cgccggtgtt ccgctcatgt ttggtgccat atcggacatg acaacctgga
    22801 ctttgctgtc gccaacgcgc tccagcagtg ctttcatcac cagttcatca cgaaaatcgc
    22861 cctgaagaaa gtccacacca acgataggat ccataggtaa aagatcgcaa gcgatgatgc
    22921 ggcctttgcc gccaatttgg gtgaccacat attgtgacca accacccgga gcagcaccaa
    22981 ggtcgacaac cgtcattccc ggtttaaaga gtttgtcact ttgctgtatt tcatcaagtt
    23041 taaaccaggc acgggaacgt aacccctttt tctgtgcctg ttgaacatat ttatcgctaa
    23101 agtgttcctg aagccagcgg ctggagctgg cagaacgctt cttacctgtc atttaacttt
    23161 cccatgggga taactcatcg taaccaattg cgtaaatttt tacgcgccta tttggtgata
    23221 tatgggagat ggcggtagaa tgacccgttt tcaatcccaa cgtaagcaaa aaatacgatg
    23281 aatctgagta ctaaacaaaa acagcacctg aaaggtctgg cacatccgct caagccagtt
    23341 gttctgcttg gcagtaatgg tttgaccgaa ggggtgctgg ccgagattga acaagcgtta
    23401 gagcaccatg aactcatcaa ggtgaaaatc gccaccgaag atcgcgaaac taaaaccttg
    23461 atcgtggaag ctatcgtgcg cgaaaccggc gcctgtaatg tacaggtcat cggtaaaacg
    23521 ctggtgcttt atcgcccaac taaagaacgt aaaatctcgc tgccacgcta agattatcct
    23581 aaagttacac acatttgctg tgtaaaacga ggggttttcc gcaggcagga gagcaaaatg
    23641 ccacgctctg ttcgttgata aaaggccgca tagcggcctt tttcctttct ttacaataca
    23701 tcaacatctt gagtattggg taattcttac aggtattcca ccttaattac ttcaaattct
    23761 acttcgccgc ccggcgtttt gatgaccaca acatcatctt cttctttgcc gatcaggcca
    23821 cgagcaatag gcgagtttac agaaatcagg ttttgtttaa agtcagcttc gtcatcgcca
    23881 acgatgcgat aagtctgttc ttcgtcagaa tccagattca gcaccgttac ggtagcacca
    23941 aaaataacgc gcccattgtt gggcattttg gtgacatcaa tcacctgcgc gttcgacagc
    24001 ttggcttcga tgtctttaat acggccttcg cagaaaccct gctgttcacg agctgcgtgg
    24061 tattcggcgt tttctttcag gtcgccatgc tcacgcgctt ccgcgatagc agcaatgatt
    24121 tcaggacggc gcacagattt cagaaaatcc agctcttcgc gtaatttttc agcgccgcgt
    24181 aaggtcatcg gaatagcttg catttgttat acctcttgaa tattcctgat ggggcaagtc
    24241 ttcacccacc ccggctgttc ggcctgcccg gcataactcc tcaccgggac cagaagcaaa
    24301 aaaataccga cccgggtaca agtcccaggt cagctacaat tcacattttg atagtcattt
    24361 taccctgaag ttcccgaagg gtcatcgttt actttatagg gcgttgcgcc gtagtatgac
    24421 ggctcgattc caggttgtta gcgcgagatt atgcgatttt ccagatttat catcggattg
    24481 accagctgta tagcgttcag tgttcaggcc gcgaatgttg atgagtacgt tactcaactc
    24541 cccgctggtg ccaaccttgc cctgatggtg caaaaagtcg gcgcgtcggc ccccgctatt
    24601 gattaccaca gtcagcagat ggcgctgccc gccagtacgc agaaagtgat tactgcgctg
    24661 gcggcgttga ttcaactcgg ccccgatttt cgtttcacca cgacgcttga aaccaaaggc
    24721 aatgtggaaa acggcgtact taagggtgac ttagtggcgc gatttggtgc cgatccgacg
    24781 ttaaaacgtc aggatattcg caatatggtc gcgactttga aaaaatctgg cgtcaaccaa
    24841 atcgatggca atgtgttgat agatacctcc attttcgcca gccacgataa agcccccggc
    24901 tggccatgga atgacatgac acaatgcttt agcgctccgc ctgccgctgc catagttgac
    24961 cgcaactgtt tctccgtctc gctctacagt gccccaaaac ctggtgatat ggcttttata
    25021 cgcgtggcat cttattaccc cgttacgatg ttcagccagg tacgcaccct cccccgaggt
    25081 tctgccgaag cgcaatactg cgaactggat gtcgtaccag gcgacctgaa ccgctttacg
    25141 ctgacgggat gcctgccaca acgttctgag ccgctcccgt tggcttttgc cgtgcaggat
    25201 ggagccagct atgccggggc aattctgaaa gatgagttaa aacaggcagg tatcacctgg
    25261 agcggaacac tgctgcgcca gactcaggtt aacgaacctg gaacggtagt tgccagtaaa
    25321 cagtcggccc cgttgcacga tctgcttaag attatgctga aaaagtcgga caacatgatc
    25381 gccgatacgg ttttccgcat gataggccat gcgcgcttca atgtgcctgg aacatggcgg
    25441 gccgggtcgg acgccgtgcg tcagatcctg cgccagcaag ccggtgtcga tattggaaac
    25501 accattattg ccgatggttc agggctttcg cggcataacc tgattgcccc cgccaccatg
    25561 atgcaggtgt tgcaatacat tgcccaacac gacaatgaac ttaactttat ctccatgctg
    25621 ccgctggcgg gctatgacgg ctctttgcag taccgtgcag gtctgcatca ggcgggcgtg
    25681 gatggaaaag tctcagcgaa aaccggttcg ttgcaggggg tatataacct ggcgggattc
    25741 attaccacag cgagcgggca acgaatggcg tttgtacaat atctttctgg ctatgcagta
    25801 gaaccggcgg atcagcgtaa tcgccgtatt ccgttagtgc gttttgaaag ccgtttgtat
    25861 aaagatattt atcagaacaa ttagtcaaaa agaaaccccg gcacatggcc ggggttttgc
    25921 tgattaacgc ttgtaaatga actcaacgcc ttcttcgtcg tcttcgtccc agtcatcatc
    25981 ccagtcctca tcatcctctt cagcaatctc ttcaagctgc tggcgatgat aatcatccca
    26041 catgaattcg actttctctg gctgtttcgc ttcttcagcc tgcacgaccg ggttttcaat
    26101 gataaaggtc atcacatccc agcagagatc tttcacgccc agtccactcg ccgcagagat
    26161 cagataatat ttatcttccc agcccagcgc ttcagcaatc gctttcgctt tctcttcagc
    26221 ttctgcctta tccagcagat cgatcttatt gaacactaac caacgcggtt tcgccgccag
    26281 atcctggctg tatttttcca gctcgctgat aataatacgc gcgttttcaa ccggatcggt
    26341 gccgtcaatc ggatcgatat cgatgaggtg caacaggacg cggcaacgtt ccaggtgctt
    26401 caggaagcgg atacccagac ctgcgccttc cgcagcgcct tcaatcagtc ctggaatatc
    26461 ggcaacaacg aagctctttt cgttgtccat tcgtaccaca cccagacttg gcaccagagt
    26521 ggtaaacgga taatccgcca ctttcggttt agccgccgat accgcgcgaa taaaggtcga
    26581 tttacccgcg tttggcatcc ccaacatacc gacgtcagcc agcagcatca gctccagcag
    26641 cagctcacgc ttatcacccg gcgtaccgtt ggttttctgc cgtggtgtac ggttaacgga
    26701 cgatttgaaa cgggtattgc ccagaccgtg ccagccgccc ttagcaacca gcagacgctg
    26761 accgtgttta gtcatatcgc ccatggtttc accagtaccc tggtcgatta cacgcgtacc
    26821 taccggcact ttaatcgtca cgtctttacc gcgcttaccg gtacaatcgc ggcttgcgcc
    26881 gttctgaccg cgctctgcac ggaaagattt ttcaaaacga taatcgataa gcgtgttcag
    26941 gttttcgtcg gcttccatcc atacgtcacc accatcacca ccgtcgccgc catccgggcc
    27001 gcctttcgga atatactttt cgcggcggaa gctcacgcaa ccattaccgc catcacctgc
    27061 aacgaccaga atcgatgctt catcaacaaa cttcatttta ttctccgtaa atcattcgcc
    27121 tgcgcggggt tgcgatacca ccgttgtatg cttacgtaat ccgccccaaa tacgatgacc
    27181 aatggcggaa tacattgcgc ccgcaaccac gacaaacgca ccgagataac ctaaaaggtt
    27241 taacatcggt ctggcgaaga aatcgggcca ggccagtgat aaaagatctg aaaaaaacag
    27301 cgtaaacagt ggggtgagcg tgatgatcgc gctcacctgc gctgcctgcc agcgagccat
    27361 cgcttccgcc agggcgccat atcctaccaa tgtattcagt ccgcaaaaaa ttaaacatgc
    27421 gagctgccag tggctaagct gcgctatcac tccaggcttt gccagaggga agagcgcaat
    27481 tgtacataaa gtgtacagta aaaacaggat ctgcggtgag gccagccgac gcaataaaac
    27541 cttttgcgcc acgccataac tcacccaaac cgtcgccgca ccgaccccaa agataactcc
    27601 ccaggtgtaa tcggtgagct ttgtaaatat ctcgaccaga ctggtgttaa aaaacatcac
    27661 caggccgctc aggagcatca atgcccctac aacctgagtg ctgcgcattt tctctttcag
    27721 gataaatacg ctggcaacca tcatgccaac tggcgagagt tgcccaatca cctgcgaagc
    27781 ggtcggactc aggtattgca aggatgagct gaacaggatg aagttcccaa acagcccggc
    27841 ggtcgccact gccaacaaaa tcaaccagcg tggcttacga aacacgcgta atggcggcaa
    27901 cctcttcttc accgcaagaa tggcaccaag gccaatactc gccatcaaga aacggtaaaa
    27961 cacgattgtc ggaggttcca tcacctccag cacctgcttc attgcgattg gcaacgcccc
    28021 ccagcaaatt gctgtggtga gcgccaaaag aatgccaatg cctgcctgct gcttcatacc
    28081 gtattccctg caaagcgcat ttgcggatta acgtcgcacc atgcgcattt ttaccggtta
    28141 tcgaatgtaa aaagccccgc aacgtgttgc ggggctttca tccgttaccg ggacgcgaaa
    28201 aacttattca gcttcgatgc tgataaattt acggtttttc gggcctttaa cttcgaattt
    28261 cactttaccg tctgctttag caaacagagt gtggtcacga ccgcaaccta cgttagcgcc
    28321 agcgtggaat ttggtaccac gttgacgaac gatgatgcta cccgccagaa cggattcgcc
    28381 accgaaacgc ttaacgccca ggcgtttagc ttctgaatcg cgaccgttac gtgtggagcc
    28441 gccagccttt ttatgtgcca tttgaaatct ctcctcaggt cttaggcgct gatgccagta
    28501 attttcacat cagtgaacca ctgacgatgg ccctgctgct tacgatagtg tttacgacga
    28561 cgaaacttaa cgattttaac tttctcgcca cgaccgtgag caacaacttc agctttgatt
    28621 acgccgccat caacgaaagg aacgccgatt ttgacttctt caccgtttgc gatcatcagc
    28681 acttcagcga actcaacagt ttcgccagtt gcgatgtcca gcttttccag gcgaacggtc
    28741 tgaccttcgc ttactcggtg ttgtttacca ccactttgga aaaccgcgta catataaaac
    28801 tccgcttccg cgcacacctt ttcaatgatt cagagtgcgc tataaatatt cacaataggg
    28861 cgcgaatatt acgcaaaacg cacgcctttg acaagtgcta cagtcaatac acgaagaaaa
    28921 aaaacacaac ttgtacggta acgtttatct gtgccatttt ttcagtacaa tcactctata
    28981 ttcctaacca taaaccctaa gttgcctttg ttcacagtaa ggtaatcggg gcgaaaagcc
    29041 cggcttttgc gatgaattta gaaaaaatca atgagttaac cgcgcaagat atggcgggtg
    29101 ttaatgcggc aatccttgag cagcttaatt ccgacgtcca gctgatcaat cagttaggct
    29161 attacatcgt cagcggcggc ggtaaacgta ttcgtccgat gattgctgta ctggctgcac
    29221 gagctgttgg ctatgaggga aatgcgcatg tcaccattgc tgccctgatc gagtttatcc
    29281 acacggcgac tctgctgcac gacgacgttg tggatgaatc agatatgcgc aggggtaaag
    29341 ctaccgccaa cgctgcattt ggcaatgccg ccagcgtgct ggtaggcgat tttatttata
    29401 cccgcgcgtt ccagatgatg accagcctcg gttcgctcaa agtgctggaa gtcatgtcag
    29461 aagccgtaaa cgtcatcgca gaaggtgaag ttctgcaact gatgaacgtt aacgatccgg
    29521 acatcactga agaaaactac atgcgcgtta tctatagcaa aaccgcgcgt ctgtttgagg
    29581 ctgccgcgca gtgttccggg attctggctg gctgtacgcc ggaagaggag aaaggcctgc
    29641 aggattatgg gcgctatctc ggcactgctt tccagttgat cgacgattta ctcgattaca
    29701 atgccgatgg cgaacagtta ggtaaaaatg tcggcgacga tctgaacgaa ggtaaaccga
    29761 cgctgccgct gctgcatgcg atgcatcatg gcacaccaga acaggcacag atgatccgta
    29821 ccgccatcga acagggtaac ggtcgccatc ttctggaacc ggttctggaa gcaatgaacg
    29881 cttgtggatc tcttgaatgg acgcgtcagc gtgccgagga agaagcagac aaagccatcg
    29941 cagcgttaca ggtgctcccg gacacccctt ggcgagaagc actcatcggc ctcgcgcaca
    30001 tcgctgttca acgcgatcgt taatcccctc ccctcatccc gcgcagcgcg cggggtggtt
    30061 ccagtaaatt ccataaaaac acttactcag ctctataaca cctgaaaaac aggctgtgca
    30121 tctttatata ttctgaatat tcacactcct tacaggaact ttttagagca atcggccatc
    30181 aggagtatag tgatgctcga cagaagaagt gttctgaatg aaagcgacta acttaaggag
    30241 tgaggaaaga tggaaagtaa tttcattgac tggcatcccg ctgacatcat tgcgggtttg
    30301 cgcaagaagg gaacgtcaat ggcggcggag tctcgcagaa atggtttgag ttcctcaacg
    30361 ctggcgaatg cattatcgcg cccatggccg aaaggagaga tgattattgc gaaagccctg
    30421 ggaactgacc cctgggttat ctggccatca cgctaccatg atccgcagac ccatgagttt
    30481 atcgacagaa cgcagttgat gcgtagctac actaaaccga aaaaatgagt ggtctggcgg
    30541 tagccccgcg aacggggctg ccagctctca gacgattatt cgcctttcac acgctcaata
    30601 tttgcaccta aagcgcgcag tttgtcttca atgcgttcgt agccacgatc gatgtgataa
    30661 atacgatcaa ccaccgtcgt cccttccgca atacatccag ccagcaccag gcttgctgat
    30721 gcacgcagat cggttgccat aacctgtgcg ccagaaagtt tttcaacacc gtgacaaata
    30781 acggtattgc tttcgatttc ggcatgcgcg cccatacggc taagctccgg cacatgcata
    30841 aagcggtttt caaagaccgt ttcggtgatg aatccggtcc cttctgccac caggttcaac
    30901 agcgtgaact gggcctgcat atcggtcggg aatgccggat gcggcgcggt acgtacgtta
    30961 acagccttcg gacgtttgcc atgcatatcc aggctaatcc agtcttcgcc gacttcgatg
    31021 tccgctccag cgtcacgcag tttcgccagc acggcgtcga gagtatctgg ctgcgcgtta
    31081 cggcagataa ttttgccgcg agaaatcgcc gccgccacca ggaaagtacc ggtttcgata
    31141 cgatccggta gcacgcgata gacaccgccg cctaaacgtt ccacaccttc gatgacgata
    31201 cgatcggtgc cctgaccgct aattttcgca cccagcgtaa tcaggaagtt cgcggtatcg
    31261 acgatttccg gttcacgcgc tgcgttttca ataatcgtgg taccttccgc cagggttgca
    31321 gcacacatga tggtcaccgt tgcgccaacg ctgactttat ccatcacgat atgtgcgcct
    31381 ttcagacgac catcgacgga agctttaacg taaccttctt ccagtttgat ggtcgcgcct
    31441 aattgttcga ggccagaaat gtgtagatca accggacgcg caccgatcgt acaaccgcca
    31501 ggcagtgaaa cttgcccctg accaaagcgc gctaccagcg gccccagtgc ccagatagaa
    31561 gcacgcatgg ttttaaccag atcgtaaggt gcgcagaata cattaacgtc gcgggcatca
    31621 atatgcacag aaccattacg ttctactttc gcacccagct ggcttagcag cttcattgat
    31681 gtatcgacgt ctttcagttt tgggacgttc tggatctcta ccggttcttc cgccagtaga
    31741 gcggcaaaaa ggataggcag agcagcattt ttagcgccgg aaattgtgac ttcgccctgg
    31801 agcttcgttg gcccctgaac acgaaattta tccatttagt ttgttctcag ttaacaattc
    31861 atatccgcta ccggcgaatc gcccatagct caaaagccgt tcagtttgcg atcgcgcgcc
    31921 cactccgcag gggtatacgc tttgatcgac acagcatgaa tgcggttatc cgcaatatat
    31981 tccatcagcg ggccatagac cgtctgctgt tttttaaccc gactcatgcc gtcaaacaac
    32041 tcacccacgg caataacctg aaagtggctg ccatcgccgg aaacgtggac ttcctggagg
    32101 gagagagcgt tcatcaacac gctctgaatt tcattatttt ccatgggatc ttcaatcatc
    32161 agttaataaa ccagcgaaac atcttagagc aaagttgcgc tggcataaat aagcaaaaag
    32221 cctcgctgat aaatcagaca aggctcgact tgcaggcagg tttgccggac aggcggttaa
    32281 cgccatatcc ggcctgaaaa aatttaacga ggcagaacat cagcaggcaa attatacaat
    32341 ttcgccaggg tatacacttt gtcgtttacc ccctgaagcg tcacattgtt gccctgcttt
    32401 ttcgccagat cgataagatg gagcagcagt gccagtcccc ccgtatccac gcgggacaca
    32461 cggctaagat cgatgcaggt aatccccttc accgcttcct cacgcatttc ccaaagcggt
    32521 agcaaaacgt cctgatccag ctctccggat aacgccagcg tgtcacccgt ctgcatccag
    32581 ctcagtgact cgctcattat tttttctctt ccagagtgat tttctgttga gaaatcgatt
    32641 tcagttgcgc agtcaggccg tcgatacctt tggtacgcag cagcgttccc cactcgtttt
    32701 gtttggtggt gatcatactg acgccttcag caatcatgtc gtaagcctgc cagttgcccg
    32761 tctgggagtt tttacgccac tggaagtcca gacgcaccgg cgggcggcca ttcggatcaa
    32821 taatggtaac gcgaatcggc acaatggttt tatcgcccag cggctgttct ggcgcaatct
    32881 gataggtttg accgtgatac atcgccagcg cctgaccgta agcctgcttc aggtactcac
    32941 ggaaagcggc aaagtaggct tcacgttgag caggggtcgc actcttgtaa tactggccca
    33001 gcaccagcgc accggcgtat ttcacctgta cgtatggcag cagttcctga tcaacaatgg
    33061 tacgcagata atccgggttg gcccgaattt gcggttgctc attcttcagg cgatcgaacg
    33121 ttttctgcgc cgcctcgtcc atcagcttat aaggattggt ctggtctgcc gcggttgccg
    33181 cactcagagg tgcaatcacc agcaaagcga ccatcattaa acgtttaaac atgcgtcggt
    33241 tctcctgaaa ttatttcgtt gtacccacag gttcagtggt ttcattatta cctggcgcag
    33301 cagctggcgc atcgccacta ttcttattgt catcgccttt actaccgtaa aggaactgac
    33361 caatgagatc ttccagcacc atcgcagact tagtgtcctg aattgtatcg ccatccttca
    33421 ggatagcagt ccccagttcc gggtcttcaa aaccgacgtt taatgccaga tattgttccc
    33481 ccagcaggcc ggaagtacga atgctcagcg aactggtatc tggaatgtgg ttataacgtt
    33541 gttcaatttc cagcgttacg cgcggcagat aggttttcgg gtccagcgta atatccgcca
    33601 cccgacccac aacaacgcca ccaatactga ccggagagcg cgctttcagg ccgccaatgt
    33661 tatcgaacgt cgcataaagc gtgtaggtcg gttcagtacg tatggacgtc acgttcgccg
    33721 ccttcaggca aacaaacagc gccgccagca gtgctgctaa taaaaagata cccacccaaa
    33781 tttcattttt tttcgtttgc atgaactcaa ttcccaaaca tcaatgcggt cagcacaaaa
    33841 tccagcccca gaacagccag agacgagtgg acaacggtgc gagtggttgc ccggctaatc
    33901 ccggcagacg tcgggatggc gtcgtagccg ttaaacaacg aaatccacgt caccgtgatg
    33961 gcgaacacca cgctcttaat cagacagttg accagatcca tacgccagtc gacggcattt
    34021 tgcattgccg accagaagaa cccgctatca atgcctttcc aactgacgcc gaccagagat
    34081 ccgccccaga tccccacggc gacgaaaata accgtcaaca gtggtaatga aataacccca
    34141 gcccagaaac ggggagaaat aacccgacgc agcggatcca ccgccatcat ctccatactg
    34201 gagagttgct ctgtagcgcg catcaggccg atttctgcgg ttagcgccga accagcacgc
    34261 ccggcaaaca acaacgcggc aaccaccggc cccagttcac gcagtagcga taacgccacc
    34321 agcataccca gactggtttc cgcactataa gtggtcagaa ccagataacc ttgcagcccc
    34381 aacaccattc cgatgaacac gccagaaacc acaataatca gcatcgacag gacgccgaca
    34441 ttatagagct ggcgcaccag cagcggcgca tgtttgcgaa attccggttt gccgaccagc
    34501 gcattgaata acattaaccc ggcccgcccg aacgttctca gggttttaat ccctttatgt
    34561 ccgagcgacg ccagcgcatt taacagcatg agtggcttaa ctccctggta aaagatcagc
    34621 gtgataatcg ccggcaggat agcggaacgg aacaggcccg tcagctatcc cgtccagaaa
    34681 ctgacgtacg cgcggatcag gattcgcctg caacgcctgg gcactgccat gagcgacaat
    34741 ttttttgtcc gccaggatcc aggcgtgatc cgcaatactt aacacttccg gcacatcgtg
    34801 agaaaccacc acacaagtca cgcccagcgc gctgttcagc tcagaaatca gcttcaccag
    34861 tacgcccatg gtgatgggat cttgcccaac aaaaggttca tcaaacatga tgagatccgg
    34921 ctccagcgca atcgcacgcg ccagcgctgc acgccgcgcc atcccaccgg aaagttcaga
    34981 aggcattagt ttagccgctc cacgcagccc cacggcctcc agcttcatca tcaccgtact
    35041 atgcaacaat ggcgcgggaa gttgggtatg ttcgcgcagt ggataggcga cgttgtcaaa
    35101 tacgttcata tcagtgaaca acgccccgga ctgaaataac atgctcatcc gtttgcgcac
    35161 tgtatacagg cgcgaacgag acatcgccgg aatattctca ccatcgaaaa ggatctcacc
    35221 atgatctggt gcgatttgcc cgccaatcag acggagtagc gtcgttttac cgatgcccga
    35281 tggccccatg atcgccgtga tcttccctcg cggcacggtc agggaaatat tatcgaagat
    35341 gcagcgattg ccacgcgtaa aactgacatc gcgcatatcg actaaattcg ccacagactg
    35401 ctccataatt cacccttcgt cttgcgttga ttttctaagc atggcgctca atttaacctt
    35461 gaacccaaca tatttacaga atattacccg ccgtggttag cgaaagctgg catttgtttt
    35521 actttttagc cgcataaagt caaaattaag aatccgttac ggctttctga aaatcttcag
    35581 cggaccggcg agtatacctg aagaaaggac gttagatgct tttagctacg gcactgttaa
    35641 ttgttggttt acttttggtc gtttacagtg ccgaccgcct ggtttttgcc gcgtctattc
    35701 tttgccgaac ctttggcatc ccgccgctga tcatcggcat gacggtggtc agtattggta
    35761 catcgttacc agaaatcatc gtctcgcttg ccgcgtctct gcacgaacaa cgcgatttag
    35821 ccgttggtac agccctcggc tcaaacatta tcaatatatt gctgatcctc ggtctggctg
    35881 cgctggttcg tccttttacc gtccattctg atgttctacg ccgtgaatta cccttaatgt
    35941 tgttggtcag cgtggtggcc ggttccgtac tctatgacgg acaacttagt cgcagcgatg
    36001 gtatctttct cctctttctg gctgtgctat ggctgctgtt cattgttaaa cttgcacgtc
    36061 aggctgaacg tcaggggact gacagcctga ccagagagca gcttgcagag ctgccgcgtg
    36121 acggcggatt gcccgtcgcg tttttatggc tcggcattgc gcttatcatc atgccagtgg
    36181 ccacgcggat ggtggttgat aacgccacgg tgctggcgaa ttactttgcc atcagcgagt
    36241 tgacgatggg tctgacggca attgctatcg gaaccagcct gccggaactg gcaaccgcaa
    36301 tagcgggggt tcgcaaaggt gaaaacgaca ttgctgtcgg aaatatcatt ggcgcaaaca
    36361 tttttaatat tgtcatcgtg ttgggtttac ccgcgctgat aacgccagga gagattgatc
    36421 cactggcgta cagtcgtgac tacagcgtga tgttgctggt gagcattatt tttgcgttgc
    36481 tgtgctggcg gcgctcccca caaccgggcc gtggtgtagg ggtattatta actggcggat
    36541 ttatcgtatg gctggcgatg ttgtactggt tatcgccaat actcgttgaa taactggaaa
    36601 cgcattatgt cgcacgtaga gttacaaccg ggttttgact ttcagcaagc aggtaaagaa
    36661 gtcctggcga ttgaacgtga atgcctggcg gagcttgatc aatacatcaa tcagaatttc
    36721 acgcttgcct gtgaaaagat gttctggtgt aaagggaaag ttgtcgtcat ggggatgggg
    36781 aaatcggggc acatcgggcg caaaatggcc gcaacgtttg ccagcaccgg tacaccttca
    36841 tttttcgtcc atcctggtga agccgcgcat ggtgatttag gcatggtcac cccacaggat
    36901 gtggtgattg ctatctctaa ctcaggtgaa tccagcgaaa tcacggcctt aattccagtg
    36961 cttaagcgtc ttcacgtacc gttaatctgc atcaccggtc gcccggagag cagcatggcg
    37021 cgcgccgcag atgtgcatct gtgtgttaaa gtagcgaaag aagcctgtcc gttagggctg
    37081 gcaccgacca gcagcaccac cgccacgctg gttatgggcg atgccctcgc tgtcgcgctg
    37141 ttaaaagcac gcggctttac tgctgaagat tttgcgctct cacacccagg cggcgcactg
    37201 ggtcgtaaac ttctgctgcg cgtaaacgat attatgcata cgggcgatga gatcccgcat
    37261 gttaagaaaa cggccagtct gcgtgacgcg ttgctggaag ttacccgcaa aaatcttggt
    37321 atgactgtca tttgcgatga caatatgatg attgaaggca tctttaccga cggtgattta
    37381 cgccgtgtct tcgatatggg cgtggatgtt cgtcagttaa gtattgccga tgtgatgacg
    37441 ccggggggaa tacgtgtgcg ccctggcatt ctggccgttg aggcactgaa cttaatgcag
    37501 tcccgccata tcacctccgt gatggttgcc gatggcgacc atttactcgg tgtgttacat
    37561 atgcatgatt tactgcgtgc aggcgtagtg taaagattca aggataaaca acaatgagca
    37621 aagcaggtgc gtcgcttgcg acctgttacg gccctgtcag cgccgacgtt atagcaaaag
    37681 cagagaacat tcgtctgctg atcctcgatg tcgatggcgt actgtcagat ggcctgattt
    37741 atatgggcaa taatggcgaa gagctgaaag cgttcaatgt tcgtgacggt tatggcattc
    37801 gttgtgcgct cacctctgat attgaagtcg ctatcattac cgggcgaaag gctaaactgg
    37861 tagaagatcg ttgtgccaca ttggggatca ctcacttgta tcaggggcag tcaaataaac
    37921 tgatcgcctt tagcgatctg ctggaaaaat tggcgattgc cccggaaaat gtggcttatg
    37981 tcggcgatga tctcatcgac tggccggtaa tggaaaaagt gggtttaagc gtcgccgtgg
    38041 ccgatgcgca tccactgttg atcccgcgcg ccgattacgt gacgcgcatt gctggcggtc
    38101 gtggcgcagt gcgcgaagtt tgcgacttat tactccacgc gcagggcaaa ctggatgaag
    38161 ccaaagggca atcgatatga gtaaagccag acgttgggtt atcattgtgc tatcactggc
    38221 ggttctggtg atgatcggca ttaatatggc cgaaaaagac gataccgccc aggtggtcgt
    38281 caacaacaat gatcccacct ataaaagcga gcatacggac acgctcgtct ataacccaga
    38341 aggggcacta agctatcgat tgattgctca acacgttgaa tattattccg atcaggccgt
    38401 ttcgtggttt acgcagccgg tacttaccac gtttgataag gataaaatcc cgacatggtc
    38461 cgtaaaagca gataaagcca agctgaccaa tgaccggatg ctctatttat atggacacgt
    38521 tgaagtcaac gcacttgtgc cagactctca acttcgcaga atcacgacgg ataacgcgca
    38581 gatcaatctg gtgactcagg atgttacctc tgaagacctc gtcacgttat acggaacaac
    38641 atttaactcc agcggtctga aaatgcgcgg caacttacgc agcaagaacg ccgagctgat
    38701 tgaaaaggtt agaacatcct atgaaattca aaacaaacaa actcagcctt aatcttgtgc
    38761 ttgccagctc acttctggcc gccagcattc cggcatttgc cgtaaccgga gacactgatc
    38821 agccgatcca cattgaatcg gaccagcaat ctcttgatat gcaaggcaac gtggttacct
    38881 ttactggtaa tgtcatcgtc acccagggca ccatcaaaat taatgccgac aaagtggtcg
    38941 ttacccgtcc gggcggcgaa caaggtaaag aagtgattga cggctacggt aaaccggcaa
    39001 cgttctacca gatgcaggac aacggtaaac ccgttgaagg tcacgcttcc cagatgcact
    39061 acgaactggc aaaagatttt gtcgttctga cgggtaatgc ttatctgcag caggtcgata
    39121 gcaacattaa gggcgataag atcacttacc tggtaaaaga gcagaaaatg caggctttca
    39181 gcgacaaagg caagcgcgta accaccgttc tggtgccgtc gcagctgcag gacaaaaaca
    39241 acaaaggcca gaccccggca cagaagaagg gtaattaatt cgttatggca acattaactg
    39301 caaagaacct tgcaaaagcc tataaaggcc gtcgcgtggt agaagacgtc agcctgaccg
    39361 tcaactccgg ggaaattgtc ggtctgctgg ggccaaacgg tgccggtaag accaccactt
    39421 tctatatggt tgttggcatt gtgccgcgcg atgcgggcaa catcattatt gatgatgacg
    39481 atatcagtct gctgcctctg catgcacgcg cgcgccgcgg tatcggctat ctgccacagg
    39541 aagcctccat tttccgtcgc ctcagcgttt acgataacct gatggcggta ctgcaaattc
    39601 gtgacgactt gtctgctgaa caacgtgaag accgcgcgaa cgagctgatg gaagagtttc
    39661 acattgagca cctgcgtgac agcatggggc agtcactctc cgggggtgaa cgtcgccgtg
    39721 tagaaattgc ccgcgcactg gctgcgaatc cgaaatttat tctgctcgac gaaccgtttg
    39781 ccggggttga cccgatctcg gttatcgaca ttaaacgcat cattgagcac ctgcgcgaca
    39841 gcggcctggg cgtgctgatc actgaccaca acgtgcgtga aacactggcg gtttgtgaac
    39901 gcgcttatat cgtcagtcag gggcatttga tcgcccacgg cacgcctaca gaaatcttac
    39961 aagacgaaca cgttaagcgt gtataccttg gggaagactt cagactctga tagggtagaa
    40021 gtttgcgacg ttttagcagg agagtacgat tctgaacatg aagcaaggtt tgcaactcag
    40081 gcttagccaa caactggcga tgacgccaca gctccaacag gcaattcgtc tgttgcagtt
    40141 gtcgacgctg gaacttcagc aggagctaca gcaggcgctg gagagtaatc cgctgcttga
    40201 gcaaatcgac actcatgaag aaatcgacac ccgcgaaacg caagacagtg aaacgctgga
    40261 caccgccgac gcgctcgaac aaaaagagat gccggaagag ctgccgctcg atgccagttg
    40321 ggacaccatt tacaccgctg gtacaccatc cggcaccagc ggtgactaca ttgacgatga
    40381 gctgccggtc tatcagggcg aaacgacgca gaccttgcag gattacctga tgtggcaggt
    40441 cgagctgaca ccgttttccg acactgaccg cgctattgct acctctatcg tcgatgccgt
    40501 tgatgacacc ggttatctga ctgtcccgct ggaagatatt ctcgaaagta tgggcgatga
    40561 agagatcgac atcgacgagg ttgaagccgt ccttaagcgg atccaacggt ttgatccggt
    40621 cggtgtagcg gcaaaagatc tgcgtgactg cctgctaatc caactctccc aattcgataa
    40681 gaccacgccg tggctggaag aggccagatt gatcattagc gatcatctcg atctgttagc
    40741 caatcacgac ttccgcactt taatgcgcgt cacgcgtctg aaagaagatg tgctgaaaga
    40801 agccgtcaat ctgatccagt cgctcgatcc gcgccccggg cagtcgatcc agactggcga
    40861 acctgagtat gtcattccag atgtgctggt gcgtaagcat aacggtcact ggacggtaga
    40921 actcaacagt gacagcattc cgcgtctgca aatcaaccag cactacgcct cgatgtgcaa
    40981 taacgcgcgc aacgatggtg acagccagtt tatccgcagc aatctgcagg atgccaaatg
    41041 gttgatcaag agtctggaaa gccgtaacga tacgctactg cgcgtgagtc gctgtatcgt
    41101 tgaacagcag caagccttct ttgagcaagg tgaagaatat atgaaaccga tggtactggc
    41161 cgatatcgcc caggctgtcg aaatgcatga atcgacgata tctcgcgtga ccacgcaaaa
    41221 atacctgcat agtccacgag gcatttttga actgaagtat ttcttttcca gtcacgtcaa
    41281 taccgagggc ggcggcgaag cttcctccac ggcgattcgt gcgctggtga agaaattaat
    41341 cgcggcggaa aacccagcga aaccgttgag cgacagcaag ttaacctctt tgctgtcgga
    41401 acaaggtatc atggtggcac gccgcactgt tgcgaagtac cgagagtctt tatccattcc
    41461 gccgtcaaac cagcgtaaac agctcgtttg acccaaccga taaggaagac actatgcagc
    41521 tcaacattac cggaaataac gtcgagatca ccgaggcact gcgcgaattt gttacagcca
    41581 aatttgccaa acttgagcaa tattttgacc gaatcaacca ggtctatgtt gttctgaaag
    41641 tggagaaagt cacccacacc tcagatgcaa cactgcatgt aaacggtggt gaaattcatg
    41701 ccagcgcgga aggtcaggac atgtacgctg ccattgatgg cttaattgat aagctggcac
    41761 gccagttgac caaacataaa gataaactga aacaacacta attgtccggg caattagcat
    41821 gtgcatggcg gcctgttgtg cggcacaacg ggccatttgt acggttaatg ctccgagcct
    41881 gttccactgt ttgagtgggc aggttcttag gtgaaattat gacaaataat gatacaactc
    41941 tacagcttag cagtgttctt aacagggaat gtacgcgaag ccgcgtccac tgtcagagca
    42001 aaaaacgcgc cctggaaatc atcagcgagc tggcggcgaa acaacttagc ctgccgcctc
    42061 aggtggtttt tgaagctatc ctgacgcggg aaaaaatggg cagtaccggt atcggcaatg
    42121 gtattgccat tccgcatggc aaactggaag aagatactct gcgtgccgtt ggcgttttcg
    42181 ttcagctcga aacgcctata gctttcgatg ccatcgacaa ccaaccggtg gacttacttt
    42241 ttgccctgct ggtgccggca gaccaaacta aaacgcacct gcatactctg tcgctggtgg
    42301 cgaaacgtct ggcggacaaa accatctgcc gccgtttacg cgcagcccag agcgatgaag
    42361 agctgtatca aatcattacg gataccgaag gtactccgga tgaagcgtag ttattcggta
    42421 atgtctcttt tagacgttgt gaggagaaac agtacatggt actgatgatc gtcagcggac
    42481 gttcaggttc aggtaaatct gtcgccctgc gtgcgctgga agatatgggt ttttactgcg
    42541 tggataacct tcccgtagtg ttgttacccg atctggctcg aaccctggcc gatcgcgaga
    42601 tttctgccgc cgtcagcatt gatgttcgta atatgccgga gtcaccagaa atattcgaac
    42661 aggcgatgag taacctgcct gacgctttct caccgcaact actgttcctg gatgccgacc
    42721 gtaatacctt aattcgtcgt tacagtgaca cgcgccgact gcatccgctt tccagcaaaa
    42781 acctgtcgct ggaaagtgct atcgacaaag aaagtgatct gctggagcct ctgcgttcgc
    42841 gagcggatct gattgtcgat acctcagaaa tgtccgttca cgagctggca gaaatgctgc
    42901 gtacccgtct gctgggtaaa cgtgaacgcg aactgacaat ggtctttgag tctttcggct
    42961 tcaaacacgg tatccctatc gatgcagatt acgtctttga cgtgcgcttc ttgccgaacc
    43021 cgcactggga tccgaaactg cgtccaatga caggtcttga taagcctgtc gccgcgttcc
    43081 tcgaccgcca cacagaagta cacaatttta tctaccagac gcgaagctat cttgagctat
    43141 ggttacctat gctggaaacc aacaaccgta gctacctgac ggtcgccatt ggttgtaccg
    43201 gcgggaagca ccgttcggtg tatattgcag agcaactggc agactacttc cgctcgcgcg
    43261 gtaaaaacgt ccagtcacgc catcgtacgc tggaaaaacg taaaccatga ccgtcaagca
    43321 aactgttgaa atcacaaaca agctgggcat gcatgcccgg cctgcaatga agctgtttga
    43381 attaatgcag ggttttgacg ctgaagtgct gttacgtaat gacgaaggca ccgaggctga
    43441 agccaacagc gttattgcgc tgctgatgtt ggattctgcc aaaggacggc agattgaagt
    43501 tgaagcgacc ggtccacagg aagaggaagc actggccgcc gttatcgccc tctttaactc
    43561 tggttttgat gaagattaat cttcatcact ttgacattca aatcccttca agctcccccg
    43621 gcaataagaa aagcgcgtac tgaaatcgtt ctcaatcaac gtcaattgta catttcgtgc
    43681 gctttttact ttcagaagaa ccttaagaaa accttaagaa gcattgttta ggtttcgttt
    43741 aagttaatcg accatactgg agatcgtcag aaaatatttc caggagatgg catgattcgc
    43801 ttatctgaac aaagtcccct gggcaccgga agacatcgga aatgttatgc gcatccggaa
    43861 gatgcccaac gctgtatcaa gattgtctac catcgtggcg atggtggcga taaagagatc
    43921 cgccgtgagt taaagtatta cgcgcatctt ggtcgccggt taaaagactg gagtggaata
    43981 ccgcgctatc acggtaccgt tgagacggat tgcggaaccg gttatgtcta cgatgtgatt
    44041 gccgattttg acggcaagcc ttccattacg ctgaccgaat ttgctgaaca atgccgttac
    44101 gaagaagaca ttgcacaact gcgccagtta ctgaaacagc tgaagcgtta tttgcaggat
    44161 aaccgtatcg tgacgatgtc gttgaagccg cagaatatcc tctgtcaccg cattagtgag
    44221 tcagaggtta tccccgtcgt ctgcgacaac attggtgaaa gcacattgat tccactggcg
    44281 acctggtcga agtggtgttg tctgcgtaag caagagcggt tatggaaacg atttattgcc
    44341 caaccggcgc tggctattgc cctgcagaaa gatctgcaac ctcgtgagag caaaacgctg
    44401 gccctcactt cgggcgaagc ttaatccagc tggtgttgtt gcataaacgg ctcaccgcct
    44461 aactgataca tctgccgtaa aatccacgcc tgacggctac gcacgtagcc cgatggcgcg
    44521 gagactttga aacgaagtgg attaggtaat acagctgcca gtaatgcagc ttccgatcgg
    44581 gtaagtttgc tcgcgggttt gtggaaataa cgttgtgccg cagcttcgac gccaaacaca
    44641 ccgtcgccaa attcggcgat attcaggtaa accgtcagga tacgcttttt gctccagacc
    44701 gtttctatcc ccagcgttaa tccggcttcc agcccttttc gaacccagct acgcccatcc
    44761 cataaaaaga gatttttggc cgtctgctga gaaatcgttg atgcaccgcg aatacggttt
    44821 tcattgcgct cgttgtgcgc cagggctttc tcaatggaag cgacatcaaa gccccagtgc
    44881 tcaggaaatt tctgatcttc tgcggcaatc accgccagtc ccatccatgg cgagatttga
    44941 tccatactga cccagtcaga atgtgccacg taacgaaaat tgccatgcag ccaggcgctg
    45001 acctgtcgct cgaccattac cgctgagaag ggaacaggcg caacgctaaa caacgcgatg
    45061 cccccgcccc agaaaacggc gaggacaacc attaaccgca aaagaaaacg gcgaacgaaa
    45121 ctaaacaccg ttaagcggct tttactcatt cagccagaac cagcacgcgg gaaaccagct
    45181 tatcaatgcc gctcgccgct tctgcaatgt tctgcgccag catatatgct ggggtggtga
    45241 caatcttatt atcttcatca accacgatat catcgacagg acacggcaca tgctccgcgc
    45301 ccatctcttc cagcacttct gcggtatcga tatcagtacc gatggtcaaa cgcagcggga
    45361 aatcgaaaat tttcggcagc atcgccgggg caatacacat aaaaccaagc ggttttccgg
    45421 cctgatgcat cgcttgtgcc agcgccttta attcacggtc aacggtgcat tcgctaccaa
    45481 gagtggcaaa attgcttaaa ttcttcgccg cgccaaaccc ccccggcaca atcaacgcat
    45541 ccagttcagc ggcatcggcc tgggccagag gacggatttc accgcgcgtt attcgtgccg
    45601 cttcaatcag cacgttgcgc gtttccgtca tcgcttcgcc agttaaatgg ttgataacat
    45661 caacctgctg cttatccggt gcaaagcaga ccgcctgcgc accgctgcgt gaaatagcta
    45721 acaacgtcaa caccgcttca tgaatttcag aaccgtcata gacgccgcat ccgctcagaa
    45781 ttacgccaat tttcttcatt gtgatcatcc tttcgcaatt gactgaaaca cattaatttt
    45841 tttaataaaa atggtacgca tcacacattt aactgattca tgtaacaaat catttaagtt
    45901 ttgctatctt aactgcgtgc ggcctgaaaa acagtgctgt gcccttgtaa ctcatcataa
    45961 taatttacgg cgcagccaag atttccctgg tgttggcgca gtattcgcgc accccggtct
    46021 agccggggtc attttttagt ggcttttgcc acccacgctt tcagcacttc tacgtcgtga
    46081 cgccactctt ctttcatctc ttcaatccat tcaccgacgt tatcttccca ggcaggaagg
    46141 tccggagact gaatttgctg acccagctgt tgcagatggc gtaaccccac tgaccccgcc
    46201 gcacctttaa ttttatgtcc ttcctctaca atgccttttt tatcctgcgc cgtcagattc
    46261 gactccagca cgctgacata gcccggcatc atcttctcaa acaccgctaa cccgtcggtg
    46321 atcagcttcg gtcctacaag ttcgagatac tgttccagca tgggaatatc gagcaatgct
    46381 tctgatttac tgttctcttc tgtcgtcacc gtactctcct catcatcctg ggtatcccag
    46441 aatttcttga tcatcgcggt tagcgccgga accgaaagcg gcttactcag cacatcatcc
    46501 attccagcat tgaggtactc ttgtttgtct ttcagcacgt tagcggttaa ggccaccagc
    46561 ggcggtaaat cctcgcgcgg ataacgcttc gtcagttcac gagagatatc cagcccggtc
    46621 atatctggca actgaatatc cagcaacacc aggtcgtatt cgcccggttt aaacatctcc
    46681 agcgccgcct tgccggtcat ggcgacatca acgctgttac ctaatttttc cagcacagaa
    46741 cgcgcaacaa tcacgttcag ttcaatgtct tccaccagca gcacattcag cgccggtaaa
    46801 ggcatatcgt cttcatcaaa cgcatcatcg acctcttctg ctaccgacgg tgcgtggatc
    46861 gtcaacgtaa aggttgaacc tttgccctgt tcgctggtaa ccgtaatatc gccgcccata
    46921 tttttcgcca gacgacgaga aacggccaga ccaataccgg tgccggtggc aggtttaccg
    46981 ccatgactgt ctttcacctg gtaatacatg gcgaaaattt tatccagctc atcctgcgga
    47041 atgccgatac cagagtcttc cacttcaaaa tgcagcatat cgccttcatc gtagcgcacg
    47101 cgcacggtaa cctggccttg ctgggtgaat ttgacggcgt tactgatgag gttccacagg
    47161 atctgccgta aacgcgtccc gtcggtaatg acctgatgcg gtaatggcag cgtcggctcc
    47221 aggttaaagc gcaatccttt ttgttgcgcc tgcaatgcgg agagattttc cagatcggca
    47281 aggaagctgg tgaaatcaac cggttgatta tcaagctgga ccttgcgccg ttccatctta
    47341 tccatgtcga taatatcgtt aaagatattc cccagcgtga cggccgaaac atggatggtc
    47401 ttgagatatt tttcctgctc ggcggtgagt tcggtatcca gcagaatacg gctcaggccg
    47461 acgataccgt tcagcggtgt acgcaattcg tgactgatgg tggagataaa cgtcgttttg
    47521 tcgcggctgg cccgttcaag cgcatcctga taccgcttac gctcggtaat gtcgcgacca
    47581 aagcccatca aaccgtgacg tttacccacg cggtcgtagt acggcacttt acggatttca
    47641 aagcaggctt tgcgcccgtc cgggtaatcc agccactgtt cataggtcag tgacacatta
    47701 tgacggaaca ctttttcatc ggtttcaatg acttttgcgg cggcttccgg tgagtaaaca
    47761 tcagcaggtt tcaggtgaac cagttgtttt tcgctttttc cggtcagcag ctccatcgcg
    47821 cggttacagc cggaaaactc tttatcttcg ttacgataaa aaaccaggtc gggtgaagca
    47881 tcaaggaagg aacgtaagaa tgaggattgc tgctcgagct gaatttgtgt ctcttcgcgc
    47941 tctttgattt caattttcag ttggccgaag gtttcctgca gttctgcttc cgctttttca
    48001 cgaacggcga tttcctgatt tagctgggca atattatctt ttaactgaac gttgaggctc
    48061 aaatcgcgct cgcgcatctc ctccagtttt tgcaccagcc gtgacagacg ttgtcgtgac
    48121 tcctccagtt gctcgacgac caccgataga aagtagaccg cccacggcgt aatcagcaaa
    48181 ccaaagaaga tagaacgaat aacatcaatg ctttcgacct gaccatgcag caccatggtt
    48241 accgccattt gtaccacaat ggcaagaacg acgagggcca gcgccagcaa cattgagaag
    48301 cgcaccagac ctaacttcat catcaggtca acataatact gcgccagcag acgaatttgc
    48361 ttcattaggg aattccttca cgacaacctg ccccaataat acccaattct gacagttacg
    48421 ttataaattg tgtgagaaat gcggaattcc cctcaacgac ctactccgtt ggaggtatcc
    48481 acggacgttc cagcgccgat ccctgtacgc catgttcatt cagatagcga tccagctcga
    48541 ccattcccgt ccatcgattt tcgcaccaca gaggagcaag cagcgtcggg cgacgggcgc
    48601 tggcggaaat gcggtggtag atcacttccg gtggcgtatg gcgaatcatt tctccggcag
    48661 tgagcgtgta atcctccagt tcaataccgt tcaaacgccc cgcttcccag gctttcgcca
    48721 taatgctgcc tttcacaata tgcagcggat gcagctttat gccgtccacg ccggtttcaa
    48781 ccactcgctc cagcgtttgc aaacattcag cctgcccttc gccaggcagg ccgacaatta
    48841 agtgggaaca caccttcagc ccgcgctgac gtgccagctg ggttgtacgc tgataacagg
    48901 caaaatcatg gccgcggttg atgcgatgca gtgttttgtc gtgggcggtt tgtagcccca
    48961 gctccagcca cacttcgtaa ccctggtcct tatattcgca aagcagatcc agcaccgcat
    49021 ccggcacgca gtccgggcgg gtaccaacac acaaaccgac aatattggcc tggctcaccg
    49081 cctgctgata catagaacgc agcacctgaa cttccgcaaa ggtgctggta tacgcctgaa
    49141 agtaggccag atagcgttta gcgcggttaa ctaaattcgc ctggtgcgcc agttgctcgg
    49201 caatggaacg atgctgctgc gcttcatcgg caaacgaggc aacattacag aatgtgcagc
    49261 caccacgccc gatggtaccg tcacggttag ggcagctaaa accgccgtgt agcgtcagct
    49321 tatgcacctt ttgcccataa cgacgggtga gatcaccacc aaacatattg actaatttct
    49381 gtaactgcat aatctgatag accgcgcctt gaaaagaggc caaagcctgc catttttagc
    49441 tcaattcggc gatgacctgg atcaatcgtc ccggcctgct tttatcaact gcataatcaa
    49501 tcaaaattac cgaaatttca tgcataatca cataaatcac ttttgtttat cttgtgtcag
    49561 atttttttat ctccttatgg attttaggca aaaacagtag catgaaacgt cattaccaat
    49621 taaggcagta taaaatgctg gttttctcgt cagttcaagg
//
LOCUS       DAZGVQ010000016        48707 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000016 DAZGVQ010000000
VERSION     DAZGVQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.016"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            425..1642
                     /gene="alaA"
                     /locus_tag="ACHLAG_000894"
     CDS             425..1642
                     /gene="alaA"
                     /locus_tag="ACHLAG_000894"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFP0654172.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1726..2325
                     /gene="yfbR"
                     /locus_tag="ACHLAG_000895"
     CDS             1726..2325
                     /gene="yfbR"
                     /locus_tag="ACHLAG_000895"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP0654173.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2384..4216)
                     /locus_tag="ACHLAG_000896"
     CDS             complement(2384..4216)
                     /locus_tag="ACHLAG_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFP0654174.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4303..4953)
                     /gene="hxpA"
                     /locus_tag="ACHLAG_000897"
     CDS             complement(4303..4953)
                     /gene="hxpA"
                     /locus_tag="ACHLAG_000897"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFP0654175.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4964..5458)
                     /locus_tag="ACHLAG_000898"
     CDS             complement(4964..5458)
                     /locus_tag="ACHLAG_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFP0654176.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5540..5995)
                     /gene="yfbV"
                     /locus_tag="ACHLAG_000899"
     CDS             complement(5540..5995)
                     /gene="yfbV"
                     /locus_tag="ACHLAG_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFP0654177.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6333..7535
                     /gene="ackA"
                     /locus_tag="ACHLAG_000900"
     CDS             6333..7535
                     /gene="ackA"
                     /locus_tag="ACHLAG_000900"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFP0654178.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7610..9754
                     /gene="pta"
                     /locus_tag="ACHLAG_000901"
     CDS             7610..9754
                     /gene="pta"
                     /locus_tag="ACHLAG_000901"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP0654179.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9944..11464
                     /gene="yfcC"
                     /locus_tag="ACHLAG_000902"
     CDS             9944..11464
                     /gene="yfcC"
                     /locus_tag="ACHLAG_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFP0654180.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11497..12039)
                     /gene="yfcD"
                     /locus_tag="ACHLAG_000903"
     CDS             complement(11497..12039)
                     /gene="yfcD"
                     /locus_tag="ACHLAG_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFP0654181.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12097..12648)
                     /gene="yfcE"
                     /locus_tag="ACHLAG_000904"
     CDS             complement(12097..12648)
                     /gene="yfcE"
                     /locus_tag="ACHLAG_000904"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFP0654182.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12704..13348)
                     /gene="yfcF"
                     /locus_tag="ACHLAG_000905"
     CDS             complement(12704..13348)
                     /gene="yfcF"
                     /locus_tag="ACHLAG_000905"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFP0654183.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13484..14131
                     /gene="yfcG"
                     /locus_tag="ACHLAG_000906"
     CDS             13484..14131
                     /gene="yfcG"
                     /locus_tag="ACHLAG_000906"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFP0654184.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14188..14550
                     /gene="folX"
                     /locus_tag="ACHLAG_000907"
     CDS             14188..14550
                     /gene="folX"
                     /locus_tag="ACHLAG_000907"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFP0654185.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14571..15464
                     /locus_tag="ACHLAG_000908"
     CDS             14571..15464
                     /locus_tag="ACHLAG_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFP0654186.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15512..16402)
                     /gene="rpnB"
                     /locus_tag="ACHLAG_000909"
     CDS             complement(15512..16402)
                     /gene="rpnB"
                     /locus_tag="ACHLAG_000909"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFP0654187.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVLMVTRLSPDDLIAQSH"
     gene            complement(16599..17372)
                     /gene="hisP"
                     /locus_tag="ACHLAG_000910"
     CDS             complement(16599..17372)
                     /gene="hisP"
                     /locus_tag="ACHLAG_000910"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFP0654188.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17380..18096)
                     /locus_tag="ACHLAG_000911"
     CDS             complement(17380..18096)
                     /locus_tag="ACHLAG_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0654189.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18093..18779)
                     /gene="hisQ"
                     /locus_tag="ACHLAG_000912"
     CDS             complement(18093..18779)
                     /gene="hisQ"
                     /locus_tag="ACHLAG_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFP0654190.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18869..19651)
                     /gene="hisJ"
                     /locus_tag="ACHLAG_000913"
     CDS             complement(18869..19651)
                     /gene="hisJ"
                     /locus_tag="ACHLAG_000913"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFP0654191.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19872..20654)
                     /gene="argT"
                     /locus_tag="ACHLAG_000914"
     CDS             complement(19872..20654)
                     /gene="argT"
                     /locus_tag="ACHLAG_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFP0654192.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20920..21489)
                     /gene="ubiX"
                     /locus_tag="ACHLAG_000915"
     CDS             complement(20920..21489)
                     /gene="ubiX"
                     /locus_tag="ACHLAG_000915"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFP0654193.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21584..23101)
                     /gene="purF"
                     /locus_tag="ACHLAG_000916"
     CDS             complement(21584..23101)
                     /gene="purF"
                     /locus_tag="ACHLAG_000916"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFP0654194.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23138..23626)
                     /gene="cvpA"
                     /locus_tag="ACHLAG_000917"
     CDS             complement(23138..23626)
                     /gene="cvpA"
                     /locus_tag="ACHLAG_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFP0654195.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23885..24547)
                     /gene="dedD"
                     /locus_tag="ACHLAG_000918"
     CDS             complement(23885..24547)
                     /gene="dedD"
                     /locus_tag="ACHLAG_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFP0654196.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24537..25805)
                     /gene="folC"
                     /locus_tag="ACHLAG_000919"
     CDS             complement(24537..25805)
                     /gene="folC"
                     /locus_tag="ACHLAG_000919"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFP0654197.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25875..26789)
                     /gene="accD"
                     /locus_tag="ACHLAG_000920"
     CDS             complement(25875..26789)
                     /gene="accD"
                     /locus_tag="ACHLAG_000920"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFP0654198.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26945..27604)
                     /locus_tag="ACHLAG_000921"
     CDS             complement(26945..27604)
                     /locus_tag="ACHLAG_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP0654199.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27687..28499)
                     /gene="truA"
                     /locus_tag="ACHLAG_000922"
     CDS             complement(27687..28499)
                     /gene="truA"
                     /locus_tag="ACHLAG_000922"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP0654200.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28499..29512)
                     /locus_tag="ACHLAG_000923"
     CDS             complement(28499..29512)
                     /locus_tag="ACHLAG_000923"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP0654201.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29578..30714)
                     /gene="pdxB"
                     /locus_tag="ACHLAG_000924"
     CDS             complement(29578..30714)
                     /gene="pdxB"
                     /locus_tag="ACHLAG_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFP0654202.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30813..31808
                     /gene="flk"
                     /locus_tag="ACHLAG_000925"
     CDS             30813..31808
                     /gene="flk"
                     /locus_tag="ACHLAG_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFP0654203.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMTLKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRVMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31805..32983)
                     /locus_tag="ACHLAG_000926"
     CDS             complement(31805..32983)
                     /locus_tag="ACHLAG_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0654204.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPVLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33248..34468)
                     /gene="fabB"
                     /locus_tag="ACHLAG_000927"
     CDS             complement(33248..34468)
                     /gene="fabB"
                     /locus_tag="ACHLAG_000927"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFP0654205.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34627..36633
                     /gene="mnmC"
                     /locus_tag="ACHLAG_000928"
     CDS             34627..36633
                     /gene="mnmC"
                     /locus_tag="ACHLAG_000928"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFP0654206.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDAGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(36754..37032)
                     /locus_tag="ACHLAG_000929"
     CDS             complement(36754..37032)
                     /locus_tag="ACHLAG_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFP0654207.1"
                     /translation="MIAEFESRILALIDDMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(37066..37614)
                     /gene="epmC"
                     /locus_tag="ACHLAG_000930"
     CDS             complement(37066..37614)
                     /gene="epmC"
                     /locus_tag="ACHLAG_000930"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFP0654208.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(37614..38423)
                     /locus_tag="ACHLAG_000931"
     CDS             complement(37614..38423)
                     /locus_tag="ACHLAG_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP0654209.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLFDSHGQEILHWLGMN"
     gene            complement(38423..39247)
                     /gene="mepA"
                     /locus_tag="ACHLAG_000932"
     CDS             complement(38423..39247)
                     /gene="mepA"
                     /locus_tag="ACHLAG_000932"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFP0654210.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(39251..40336)
                     /gene="aroC"
                     /locus_tag="ACHLAG_000933"
     CDS             complement(39251..40336)
                     /gene="aroC"
                     /locus_tag="ACHLAG_000933"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFP0654211.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(40371..41303)
                     /gene="prmB"
                     /locus_tag="ACHLAG_000934"
     CDS             complement(40371..41303)
                     /gene="prmB"
                     /locus_tag="ACHLAG_000934"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP0654212.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            41469..42020
                     /gene="smrB"
                     /locus_tag="ACHLAG_000935"
     CDS             41469..42020
                     /gene="smrB"
                     /locus_tag="ACHLAG_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFP0654213.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(42093..42947)
                     /locus_tag="ACHLAG_000936"
     CDS             complement(42093..42947)
                     /locus_tag="ACHLAG_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFP0654214.1"
                     /translation="MKILRHVLCLLLLLMFNTCGQAATKVGELYVPAGASSPATEFEF
                     HLIIATPDSVNYAIFKATFMPNSQPDLVSWTGDSSTQPSMSKISDSRVSMSACPGLEQ
                     YDSQTKTGWTCNELKMFVYYDGNLHGCPWIVSSFVKSRDPFAKTYDDDFPDYIGPTKV
                     SSSCPAVPLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMENGKLCNGNNFDE
                     RGAYCRFIAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDTTSRQPIDSTCR
                     FTYILNMY"
     gene            complement(42949..43488)
                     /locus_tag="ACHLAG_000937"
     CDS             complement(42949..43488)
                     /locus_tag="ACHLAG_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0654215.1"
                     /translation="MKTAHIYSPGYALLAGLLLTASTGAQAVDNNLHFYGNLLSKSCT
                     LVVDGANLAEIHFPTVSNRDLMVSGQSAHLPVVFKLKDCKGPAGYNVKVTLSGTEDSG
                     QPGFLAVDDTSTAQGVGIGMETLDGKRVDINNTTGATFALTNGNNDINFRAWIQAKSG
                     RDVTIGDFTASLTATFEYI"
     gene            complement(43485..43973)
                     /locus_tag="ACHLAG_000938"
     CDS             complement(43485..43973)
                     /locus_tag="ACHLAG_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0654216.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            complement(43970..44479)
                     /locus_tag="ACHLAG_000939"
     CDS             complement(43970..44479)
                     /locus_tag="ACHLAG_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0654217.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            complement(44495..45247)
                     /locus_tag="ACHLAG_000940"
     CDS             complement(44495..45247)
                     /locus_tag="ACHLAG_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0654218.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFICTGNSCQVDEEQSRKG"
     gene            complement(45267..47912)
                     /gene="yfcU"
                     /locus_tag="ACHLAG_000941"
     CDS             complement(45267..47912)
                     /gene="yfcU"
                     /locus_tag="ACHLAG_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFP0654219.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLKSDIFDGFNYVG
                     GSVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDS
                     VSGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSG
                     AEASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYG
                     KGSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKE
                     MYTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGY
                     RYEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVG
                     TSDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEA
                     TQSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDD
                     DGNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPA
                     VKPAIQEQTQRVTPTEPPTSISVNQ"
     gene            complement(47994..48557)
                     /locus_tag="ACHLAG_000942"
     CDS             complement(47994..48557)
                     /locus_tag="ACHLAG_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0654220.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        11932 a        13164 c        12149 g        11462 t
ORIGIN      
        1 tattaaataa taattaatta gcatcatcct cattcattaa ttccgcttaa caatagtttc
       61 acaccttgcc cccattacga cacctcggaa atcatcacgc tgttaaactc tccgtaatgc
      121 ggaaatatca tcatcgcgtt tcattagtga attcttaaat gaggcatttt cacacaatta
      181 tcttacagat aaaaaaacca gacttacaat taagaatcag aacaattcac tatataacat
      241 tgcatgtaaa gcatatacac ctcattattt tgtcattatt aagttattaa cagcacaatc
      301 gagccttccc ctctggcaaa atcttattct gcagaccttc aaaacaccgt cctgggggag
      361 tacattgttc taagctgact tccacggcag ggagtggcga taacagcaaa aaaggtcaag
      421 attcatgtcc cccattgaaa aatccagcaa attagagaat gtctgttatg acatccgtgg
      481 tccggtgctg aaagaagcaa aacgcctgga agaagaaggt aacaaggtac tgaaactgaa
      541 catcggcaac ccagccccgt tcggttttga cgcgccagat gaaatcctcg ttgacgtgat
      601 acgcaacctg cctaccgctc aagggtattg cgattccaaa ggtctttact ccgcgcgtaa
      661 agccatcatg cagcactacc aggctcgtgg catgcgtgat gttaccgtgg aagatattta
      721 catcggcaat ggtgtatcgg agcttatcgt tcaggcaatg caggcattgc tgaacagcgg
      781 ggacgaaatg ttggttcctg caccagatta cccactatgg accgcggcgg tttcgctttc
      841 cagcggtaaa gcggtgcatt atctttgcga tgaatcctct gactggttcc cggacctcga
      901 tgatattcgc gctaaaatta cgcctcgtac gcgtgggatc gttataatca acccaaataa
      961 cccaaccggc gcggtatatt ccaaagagct tttaatggag attgtggaga ttgcacgtca
     1021 gcataatctc attatcttcg ccgatgaaat ttatgacaaa attctttacg acgacgctga
     1081 gcatcactca attgcgccgc tggcacctga cctgctgacc attaccttta acggactgtc
     1141 gaaaacgtac cgcgttgcag gcttccgtca ggggtggatg gtgttgaacg ggccgaaaaa
     1201 acacgccaaa ggctacatcg aaggtctgga aatgctggct tcaatgcgcc tgtgtgctaa
     1261 cgttcctgcg caacacgcca ttcagaccgc gctgggtggt tatcagagca tcagtgaatt
     1321 tattacccct ggcggtcgtc tttatgagca gcgtaaccgc gcgtgggaac tgatcaacga
     1381 tattccgggc gtttcctgcg tgaaacctcg tggtgcgctg tatatgttcc cgaaaatcga
     1441 cgccaaacgc tttaacattc acgacgatca gaaaatggtg ctggatttcc tgttgcagga
     1501 aaaagttctg ttggtgcaag ggacggcatt caactggccg tggccggatc acttccgcat
     1561 tgtcacgcta ccgcgtgtcg atgatatcga gctgtctttg agcaagttcg cgcgtttcct
     1621 ttctggttat catcagctgt aatcttaatt tcactgccgg agattgcatc cggcagcgtt
     1681 atcccgccac aatgacctga tgatgtcatc atacgtaagg tcactatgaa acagagccat
     1741 ttttttgccc atctctcccg cctgaaactc attaaccgct ggccgctcat gcgcaacgtg
     1801 cggacggaaa atgtgtccga acacagtttg caggtagcga tggtcgccca tgcgctggca
     1861 gctatcaaaa atcggaaatt tggcggtaat gtcaacgccg aacgtatcgc tttactggcg
     1921 atgtaccacg atgcctcaga agtgctcacc ggcgatctcc ctactccggt gaaatacttc
     1981 aattcgcaaa tcgctcagga gtacaaggct attgaaaaaa tcgctcagca aaaactggtc
     2041 gatatggttc cggaagagct gcgggatatc tttgcgccgt taattgacga gcatgcatat
     2101 agcgatgaag aaaaatcgct ggtgaaacag gcagatgcac tgtgtgcata tctgaaatgt
     2161 ctggaagaac tcgcggccgg aaataatgaa ttcttgctgg caaaaacgcg actggaagcg
     2221 acgcttgaag cgcgtcgcag ccaggagatg gactacttca tggaagtatt tgttcccagc
     2281 ttccatcttt cgctcgatga gattagccag gattcaccgc tgtaagcagc cggagtctgc
     2341 gtcgcatcag gcaataagcg ccggatgcga catcaggctc ttgtcaaaac ggaaacagca
     2401 tcgggatcat caccacacaa accgccatca cgataatggt gaacggtacc cccaacttca
     2461 caaagtcact gaagctgtaa tttcccggac ctaaaaccag tgtgttaaca ggtgaagaaa
     2521 ccggtgtcat aaaggcggcg gatgctgcca tcgccacgac catcgcgaat ggataaggcg
     2581 acacccccat cgttttggca gcagccagcg caatcggagc catcaacacc gccgtcgcgg
     2641 tattagagat aaatagccca ataaccgccg acaagacaaa caaacacccc agcatcatat
     2701 gtggcccgta accaccgcca atgtccatca gccctttcac cgccagcgcg acacctcccg
     2761 ttttctgtaa tgccacagca aacggcatca tcccaacgat caaaataatg ctcggccagt
     2821 gaatggattt ataggcgctt tcagcatcta tacagcggaa tttccccatc agcaggcagg
     2881 cgatgatagc ggcaacagga ttaggaattt catctgtcag cattaacgcc accattagca
     2941 ccagacagaa aatggcatgg ggtgcctggc tgtgcgcggg cgatgcttca ctcacctctt
     3001 ccggtaagtt cagcgctacg aagtcgcggc cctgtttggc cagcataccg atcagtttcc
     3061 agttacccac aaccaggatg atatcgccca gcagcagagg ctcatccgcc agcgaacctt
     3121 ccagcgccac gccattgcgc tttagcccca ccacattcag tccgtagcgg gtacgaaaac
     3181 caatttcgcg caccgattta ccaatcagtt ctgactcagg aattaatgaa atctctgcca
     3241 tgcccacatc aagggcctgg tcagaaaaat actcgccgcg cagtaccatc ggctccagca
     3301 attgctcact acaaaattgc cggagatcga catcagccgc agacatatca ataagcaaaa
     3361 cgtcacgcgc gcgaaattca gaaaccccat taacgttcac gataacgcga cgaaaacgcc
     3421 gccagcgttc aacaccgatg acgttagcgc cataacgctc acgtaatttg agatcatcca
     3481 gccgttgacc tatcattggc gatccggggc gaatagccag acgtcgcgca cgcccggtca
     3541 gtcgatattc acggataaga tcgcgaaagg ttcgacgcgt ccagccttcg cgctgcgggg
     3601 tctgggtatc ccctttcagc atgaaacgca tcactaacat atacaaaata cccagcacca
     3661 gcacgaccag gccaataggt gttacgctaa agaaactgaa gccgtgatag ccttcacgca
     3721 gcaattcact gttgactacc aggttcggcg gcgtcgccac cagcgtcatc atgccgctaa
     3781 tcagcccggc aaaacttaac ggcatcatca gacgtgacgg cgaagtttgc atacgcatgg
     3841 caacgcttaa caccacggga ataaagatag cgacaacgcc ggttgaactc ataaacgcgc
     3901 caagccccgc gacggtcagc atcaacaaaa ccaacatttt gatttcactg ttgcccgcca
     3961 ctttgaccag ccatgttccc attacggtgg caacaccggt acggaccaaa ccatcgccaa
     4021 taataaacaa ggcggcaatc aggacaacgt taggatcaga aaagccggaa aatacttcgg
     4081 ggaccgtcag cgttccgctt aatgcaaacg cgacaataac aaacaaagcg accgcatcca
     4141 tacgcactct gcccgtcgca aacaagacga tggcaacggc cagtaatgaa agaacccaaa
     4201 tcaattcacc gttcacaact tatccttgtt aattgagggg gatgacttga ttctgccata
     4261 aaaaagcccc gacgagacgg ggctaaatca tgatcaggtg tttcactgaa taataacatc
     4321 gccatttggc tgtttggtca cagtaatttg ctccagactg tggaggacca aatcgacctc
     4381 attcaggcgc ggggtatctg ccggagcgtt aaccgcaatg acatggcaac ccgccgccag
     4441 gccagaaagc acgccagcgg gagcatcttc caccaccaca cactcctgcg gcgcaagccc
     4501 cagcagctgc gcacctaaca gatacgcatc aggttctggt tttccgcgct tcactcgctc
     4561 agcggttaca aacacctctg gtgcgggaag cccagctatt ttatggcgcg ctcgcgctac
     4621 cggcatggag ccagaagtca caatggccca cggaatacct gctttattca aatgattgag
     4681 taaggcgatt gcccccggaa gcgcggtaat accttcggtt tccgtggcct cgatgtgctc
     4741 cagacgcgta aactcggcgg caatatcagc ctcggatttg cccgccataa aatggcgcaa
     4801 agaggtgatc gcctgtttac cgtgaatgaa agccagcacc tcttccggcg ctaacccatg
     4861 acgtctggcc cagttgctcc acgcccgttc taccgcaggc agggaatcca ccagcgttcc
     4921 atcaagatca aacagaaaac ctttgcaccg cacgcgggcc tcctcaggcg ttgataattt
     4981 gattaatttc gttggcgctc aaatggtact gacgcgggca ggcatgccac acattaagca
     5041 tgcgctgata tttttcccac attggcgtct gggcgttaaa accgtgagtt ccggcgtcaa
     5101 aatgggtata gcgcccttcc acattaacca taaagcggac ataaccaagg taacgtgctt
     5161 cagtggcggc gtcaaagccg aggaaggtga cacgacgttc atcgatggat tgctggtcct
     5221 gcaaattaga ccaggaaaca tgcaacgcat gatacatctc cataatgtcg atgatagtgc
     5281 ggcaggtttc ttctttcagc tcgccaaact cgcgatccaa ttcacgcatc tgtaatccgt
     5341 aaccacgctc aataattgtt tgcaggcgac ggtaacgttc agcatttgcc ggatcgagca
     5401 tagtcatcat cttgtactga ttagacaaaa taagacgttg cgcgttggtc atttccattg
     5461 ttgactcctg tatcactcta ctacggtgaa aaaaagaagg ctgagtatgc cttcttttat
     5521 atgcgtaatc aggggtcaat tacaaatcat caaggaaagt tttatccagt tgtttgaagg
     5581 cgcgcttaag cgtgtcagct aatgcctggt aatcaggctt gccttcaacg ggtgccaaca
     5641 cctgtccaga ctcctgcaat ttaccgcgaa cttcataaaa ccagttgagg atagcagggg
     5701 gtaatggcgt tacagaacgc ttgcccagcc accataatcc ctgcatgggt aaacttaagg
     5761 cgaacagcgc agtggcaact gccggcccaa gctgaccgcc cagggcaatc tgccagcaga
     5821 gagtaaatac ggcgatcggc ggcataaaac ggatcgcata acgcgtcatc ttgataacgc
     5881 gattttcgac aaagaccggg gcaaggcgtt tttccagcgg ccacgtcttt gagtaatgct
     5941 gtccccggcg aaacaagcta aaaaaattaa cagaacgatt atccggcgtt gacatgcttc
     6001 acctcaactt cacatataaa gattcaaaaa tttgtgcaaa ttcacaactc agcgggacaa
     6061 cgttcaaaac attttgtctt ccatacccac tatcaggtat cctttagcag cctgaaggcc
     6121 taagtagtac atattcattg agtcgtcaaa ttcatataca ttatgccatt ggctgaaaat
     6181 tacgcaaaat ggcatagact caagatattt cttccatcat gcaaaaaaaa tttgcagtgc
     6241 atgatgttaa tcataaatgt cggtgtcatc atgcgctacg ctctatggct ccctgacgtt
     6301 tttttagcca cgtatcaatt ataggtactt ccatgtcgag taagttagta ctggttctga
     6361 actgcggtag ttcttcactg aaatttgcca tcatcgatgc agtaaatggt gaagagtacc
     6421 tttctggttt agccgaatgt ttccacctgc ctgaagcacg tatcaaatgg aaaatggacg
     6481 gcaataaaca ggaagcggct ttaggtgcag gcgccgctca cagcgaagcg ctcaacttta
     6541 tcgttaatac tattctggca caaaaaccag aactgtctgc gcagctgact gctatcggtc
     6601 accgtatcgt acacggcggc gaaaagtata ccagctccgt agtgatcgat gagtctgtta
     6661 ttcagggtat caaagatgca gcttcttttg caccgctgca caacccggct cacctgatcg
     6721 gtatcgaaga agctctgaaa tctttcccac agctgaaaga caaaaacgtt gctgtatttg
     6781 acaccgcgtt ccaccagact atgccggaag agtcttacct ctacgccctg ccttacaacc
     6841 tgtacaaaga gcacggcatc cgtcgttacg gcgcgcacgg caccagccac ttctatgtaa
     6901 cccaggaagc ggcaaaaatg ctgaacaaac cggtagaaga actgaacatc atcacctgcc
     6961 acctgggcaa cggtggttcc gtttctgcta tccgcaacgg taaatgcgtt gacacctcta
     7021 tgggcctgac cccgctggaa ggtctggtca tgggtacccg ttctggtgat atcgatccgg
     7081 cgatcatctt ccacctgcac gacaccctgg gcatgagcgt tgacgcaatc aacaaactgc
     7141 tgaccaaaga gtctggcctg ctgggtctga ccgaagtgac cagcgactgc cgctatgttg
     7201 aagacaacta cgcgacgaaa gaagacgcga agcgcgcaat ggacgtttac tgccaccgcc
     7261 tggcgaaata catcggtgcc tacactgcgc tgatggatgg tcgtctggac gctgttgtat
     7321 tcactggtgg tatcggtgaa aatgccgcga tggttcgtga actgtctctg ggcaaactgg
     7381 gcgtgctggg ctttgaagtt gatcatgaac gcaacctggc tgcacgtttc ggcaaatctg
     7441 gtttcatcaa caaagaaggt acccgtcctg cggtggttat cccaaccaac gaagaactgg
     7501 ttatcgcgca agacgcgagc cgcctgactg cctgatttca caccgccagc tcagctggcg
     7561 gtgctgtttt gtaacccgcc aaatcggcgg taacgaaaga ggataaaccg tgtcccgtat
     7621 tattatgctg atccctaccg gaaccagcgt cggtctgacc agcgtcagcc ttggcgtgat
     7681 ccgtgcaatg gaacgcaaag gcgttcgtct gagcgttttc aaacctatcg ctcagccgcg
     7741 taccggtggc gatgcgcccg atcagactac gactatcgtg cgtgcgaact cttccaccac
     7801 gacggccgct gaaccgctga aaatgagcta cgttgaaggt ctgctttcca gcaatcagaa
     7861 agatgtgctg atggaagaga tcgtcgcaaa ctaccacgct aacaccaaag acgctgaagt
     7921 cgttctggtt gaaggtctgg tcccgacacg taagcaccag tttgcccagt ctctgaacta
     7981 cgaaatcgct aaaacgctga atgcggaaat cgtcttcgtt atgtctcagg gcactgacac
     8041 cccggaacag ctgaaagagc gtatcgaact gacccgcaac agcttcggcg gtgccaaaaa
     8101 caccaacatc accggcgtta tcgttaacaa actgaacgca ccggttgatg aacagggtcg
     8161 tactcgcccg gatctgtccg agattttcga cgactcttcc aaagctaaag taaacaatgt
     8221 tgatccggcg aagctgcaag aatccagccc gctgccggtt ctcggcgctg tgccgtggag
     8281 ctttgacctg atcgcgactc gtgcgatcga tatggctcgc cacctgaatg cgaccatcat
     8341 caacgaaggc gacatcaata ctcgccgcgt taaatccgtc actttctgcg cacgcagcat
     8401 tccgcacatg ctggagcact tccgtgccgg ttctctgctg gtgacttccg cagaccgtcc
     8461 tgacgtgctg gtggccgctt gcctggcagc catgaacggc gtagaaatcg gtgccctgct
     8521 gctgactggc ggctacgaaa tggacgcgcg catttctaaa ctgtgcgaac gtgctttcgc
     8581 taccggcctg ccggtattta tggtgaacac caacacctgg cagacctctc tgagcctgca
     8641 gagcttcaac ctggaagttc cggttgacga tcacgaacgt atcgagaaag ttcaggaata
     8701 cgttgctaac tacatcaacg ctgactggat cgaatctctg actgccactt ctgagcgcag
     8761 ccgtcgtctg tctccgcctg cgttccgtta tcagctgact gaacttgcgc gcaaagcggg
     8821 caaacgtatc gtactgccgg aaggtgacga accgcgtacc gttaaagcag ccgctatctg
     8881 tgctgaacgt ggtatcgcaa cttgcgtact gctgggtaat ccggcagaga tcaaccgtgt
     8941 tgcagcgtct cagggtgtag aactgggtgc agggattgaa atcgttgatc cagaagtggt
     9001 tcgcgaaagc tatgttggtc gtctggtcga actgcgtaag aacaaaggca tgaccgaaac
     9061 cgttgcccgc gaacagctgg aagacaacgt ggtgctcggt acgctgatgc tggaacagga
     9121 tgaagttgat ggtctggttt ccggtgctgt tcacactacc gcaaacacca tccgtccgcc
     9181 gctgcagctg atcaaaactg caccgggcag ctccctggta tcttccgtgt tcttcatgct
     9241 gctgccggaa caggtttacg tttacggtga ctgtgcgatc aacccggatc cgaccgctga
     9301 acagctggca gaaatcgcga ttcagtccgc tgattccgct gcggccttcg gtatcgaacc
     9361 gcgcgttgct atgctctcct actccaccgg tacttctggt gcaggtagcg acgtagaaaa
     9421 agttcgcgaa gcaactcgtc tggcgcagga aaaacgtcct gacctgatga tcgacggtcc
     9481 gctgcagtac gacgctgcgg taatggctga cgttgcgaaa tccaaagcgc cgaactctcc
     9541 ggttgcaggt cgcgctaccg tgttcatctt cccggatctg aacaccggta acaccaccta
     9601 caaagcggta cagcgttctg ccgacctgat ctccatcggg ccgatgctgc agggtatgcg
     9661 caagccggtt aacgacctgt cccgtggcgc actggttgac gatatcgtct acaccatcgc
     9721 gctgactgcg attcagtctg cacagcagca gtaatctcgt catcatccgc agctttgcgc
     9781 tgcggatatc tgaaccggaa ataatcacta tttccggttt tttattctct taatctgcat
     9841 taatcctttc tgattatctt gcttaactgc gctgcatcaa tgaattgcgc catttcactt
     9901 tgcatactta ccactttgtt ttgtgcaagg gaatatttgc gctatgtccg caatcactga
     9961 atccaaacca acaagaagat gggcaatgcc cgatacgttg gtgattatct tttttgttgc
    10021 tattttaacc agccttgcca cctgggtagt tccggtgggg atgtttgaca gtcaggaagt
    10081 gcagtatcag gttgatggtc aaacaaaaac acgcaaagtc gtagatccac actcatttcg
    10141 cattctgact aacgaagcag gcgaacctga gtatcaccgc gtacagctgt tcacgacggg
    10201 cgatgaacgc ccgggcctga tgaacttccc gtttgaagga ttaacctcag gatcgaaata
    10261 cgggacagct gttggcatca tcatgtttat gctggtgatt ggcggcgcgt ttggcattgt
    10321 gatgcgtaca ggaaccattg ataacggtat cctggcgctt attcgccata cccgcgggaa
    10381 tgaaattctc tttattcctg cgctgtttat tctgttttca cttggcggcg cggtatttgg
    10441 tatgggagaa gaggccgtcg cctttgccat tatcatcgca ccgctaatgg tccggctggg
    10501 ctatgacagt attaccaccg tcctggtgac ctatattgcc acgcaaatcg gttttgccag
    10561 ttcgtggatg aacccgtttt gtgtggtcgt tgctcagggg attgccggcg ttccggtgct
    10621 ttctggctcc gggttgcgca tcgtggtgtg ggttatcgcc actatgattg gcctgatctt
    10681 taccatggtg tacgcctcac gagtgaaaaa gaatcctctt ctgtcacgcg tgcatgagtc
    10741 cgaccgcttc tttcgtgaaa agcaggcgga tgttgaacaa cgtccgttta cctttggtga
    10801 ctggctggta ttgattgtcc tgaccgccgt aatggtctgg gtgatttggg gcgtgatcgt
    10861 taatgcctgg tttattccag aaattgccag ccagttcttc accatgggtc tggtgattgg
    10921 catcatcggc gttgttttcc gccttaacgg catgacggtt aataccatgg cttcatcctt
    10981 taccgaaggg gcgcgaatga tgatcgcccc tgccctgctg gtgggtttcg ccaaagggat
    11041 tttgctgctg gtcggtaatg gtgaagcggg tgatgccagc gtgttaaata ccatcctcaa
    11101 cagcattgcc aatgccatta gcggtctgga caacgcggtc gcggcctggt ttatgttgct
    11161 cttccaggcg gtatttaatt tcttcgtgac gtccggttct ggtcaggcgg cgttaaccat
    11221 gccgttactg gcaccgcttg gcgatctggt cggtgttaac cgtcaggtta ccgtgctggc
    11281 attccagttt ggtgatggct tcagtcacat catttaccca acctcagctt cgttaatggc
    11341 aactctcggt gtatgcaggg tggacttccg taactggctg aaggtgggcg cgaccctact
    11401 tggactgctg tttattatgt ccagcgtcgt agtgatcggc gctcagttga tgggctacca
    11461 ctaaaaatgt taagagccgc aattgcggct cttttttcat tctgccgttt cagtctctac
    11521 ggcttcattt ttggcattgc gcttcatcca caacgccagc gctttcagcg agtctggagt
    11581 gaactcatcg cagcgtgcgg tgatttcttc cggcgtcagc cagcaaactt cactgacttc
    11641 atcttcctgt agggcgaagg gaccgtgaga gacgcagctg aacaacgcgc cccagacacg
    11701 gcaattttta tcttcgaaat agaactgccc gtgctcggca aaggggacac cggcaatgcc
    11761 caactcttct tccgcttcgc gacgcgcgga ttccagcagt tgctcatcgg cctgaaccac
    11821 accgcctgcg gtcgcatcta acatgccggg taaaaagtct tttgtctcgg tacgacgctg
    11881 gaccagaatt ttgcccatgc catcatgcac gacgatgtaa gttgcacgat gacgcagaca
    11941 ctgtgcccgc atttgttccc ggctggcttg tgcaatgact tcgttctctt cattgacaat
    12001 atccacccat tcagtacttg ccaaacgacg ctgttccatt atcaggaaac cttctttttc
    12061 tggcgctctt acggcgcatt ttgagttgtg ggtaaattac ggattaatcg cgacctgcgc
    12121 aatgatactt tgatcattga gtgcgattac actaagtacg tcattatcca gcatgccata
    12181 actcgccgga ttaccgcctt tcggaatact caccgagccg gggttgaagt ggaaaatctc
    12241 cccccgctgt tctgccactg gcagatgggt atgaccgtac accagcacat cgttctggtt
    12301 taaagcaggt agattttccg ggccaaaaag atgaccatgc gtcaaaaaca gacgttgttt
    12361 ttccagtaat acctgttgcc acggcgcggt tatcgggaaa tgcagcagca tttgatccac
    12421 ttcgctgtcg cagttgccgc gcacagcgat aaccttatgt gccacttcat taagccgttc
    12481 agcgactttg gctggcgcgt aaccctccgg taaagcatta cgcgggccgt gattcaacac
    12541 gtcgccaagg atcaccagcc actgggcacc gctttgggca aacaactcca gaacacgttc
    12601 cgtcgccggt aacgacccat gaatgtccga tgcaaacatc agtttcatca ctcactcctc
    12661 gtcgaaaaaa cgtaagctat gatactggat tctgtcgttg ctatcagcca gattgcttcg
    12721 ccgaaagtgc aataaaacgc tggacagacg ctcgctgcca ctggaatgtc gcataatcca
    12781 ccaagcgttc tggcacctca tcgccatgta gtaccaggcg gttaatcatt agcgccagat
    12841 cagtatcagc aatgcaccat tcaccaaata aattcggctg acccagtact aacaaatgtt
    12901 ctgccatcgc aaacagtttc tctgcactgg cttttccctc ggccgttagt ggcgcttttt
    12961 tcgcccccgc aaagacaaca tccgtcggac gctcttcgcg gatgggcatc agatcgctgc
    13021 gcagccaggc ctgaatctgt cgtgcacgcg cacgattttc taaatcaagc ggataaatac
    13081 gttcccaggt cggtggcgca aatcgatctt ccagatactc cgcaatggca gaagattcac
    13141 tcaactcaaa atcatcgatt tgtaataacg gcacacggcg tgtctgaccg taaccttgcc
    13201 acgtcggctg caaatgttca ccgctgtcga ggtcgatggt cttgatatga aacgacaggc
    13261 ctttttcctg caacgccacc caggcggata acacataagg ggagaaaaag tgggcatctg
    13321 accaaagcgt gatagcgggt ttactcatta tgtcctcggc aataaagggg gctggctatc
    13381 aaactataga gcctttttgt cactgtcacg tgacgaatac tcacgtaagt cgccgcgctt
    13441 tctatacttg tggtgttcag atgattaaac aaccggagct gcaatgatcg atctctattt
    13501 cgccccgaca cccaatggtc acaaaattac gctgtttctc gaagaagcag agcttgatta
    13561 tcgcttgatt aaggtagacc tggggaaagg cggtcagttt cgtccggaat ttttgcgcat
    13621 ttcgcccaac aacaaaattc cggcaattgt tgatcattct cctgccgatg gcggcgaacc
    13681 gttaagcctc tttgagtctg gtgccatttt gttgtatctg gctgagaaaa caggactctt
    13741 tttgagtcat gaaacgcgtg agcgcgccgc cacattacag tggttattct ggcaggtagg
    13801 cggactgggg ccgatgcttg ggcaaaatca tcattttaat cacgcagccc cccaaaccat
    13861 tccttacgct attgaacgtt atcaggttga aactcagcgt ctttaccatg tactgaacaa
    13921 gcggctggaa aactcgccct ggctgggagg cgagaactac agcattgcgg atattgcctg
    13981 ctggccgtgg gttaatgcct ggactcgcca gcgaattgac ctcgcaatgt atccggcagt
    14041 caagaactgg catgagcgga tccgttcgcg ccctgccacc gggcaggcac tgctaaaagc
    14101 acaactcggt gatgagcgtt cggatagtta acagaaacag gttctcgtgt attatttcat
    14161 cctaagtaaa acaacggaga acctgcaatg gcacaacctg ccgctattat tcgtataaag
    14221 aaccttcgtt tacgtacgtt tatcggaatt aaggaagaag aaattaacaa ccgtcaggat
    14281 attgttatca atgtgacgat ccactacccc gccgataaag cgcgcactag cgaagatatc
    14341 aacgatgcgc tgaattatcg caccgtaacg aaaaacatta ttcagcatgt agagaataac
    14401 cgtttctctt tgctggaaaa attaactcag gatgtgctcg atatcgcacg tgaacatcac
    14461 tgggtgacgt atgctgaagt ggagatcgat aaactgcacg cgctgcgcta cgccgattcg
    14521 gtatccatga ccttaagctg gcagcgttaa ccgccatatc gggaggctgc atgaacatag
    14581 tgatcaccgg agggacggga ttaattggtc gccatttgat tccgcgtttg ctggagctgg
    14641 gccatcaaat cacggtagtg acgcgtaacc cgcagaaagc cagttccgtt ctcggccctc
    14701 gggtgacgct atggcaaggg ctagccgatc aaagcaacct caacggcgtt gatgcggtaa
    14761 tcaacctggc cggagaaccg attgctgata aacgctggac tcacgagcaa aaagagcgtc
    14821 tctgccaaag ccgctggaat atcacgcaaa aactggtcga tttgattaat gccagcgaca
    14881 cgccaccgtc ggtactcatt tccggctcag caacgggcta ttatggcgac ttaggtgaag
    14941 tggtggttac cgaagaggaa ccgccgcata acgaatttac ccataaactc tgcgcccgct
    15001 gggaagaaat tgcctgccgg gcgcaaagtg acaaaacgcg agtgtgcctg ctgcgtaccg
    15061 gcgtagtgct ggcaccagat ggcggtattc tcggtaaaat gctgccgccg tttcgtcttg
    15121 gcctgggcgg gccgattggt tccggtcggc agtatctggc ctggattcat atcgatgata
    15181 tggtcaacgg cattctctgg ctgctggata acgagctgcg cgggccattt aatatggttt
    15241 cgccctaccc gatacgcaat gaacaatttg cccatgcgct cggtcatgcg ctgcatcgcc
    15301 cggctatttt gcgcgtcccc gcaaccgcca ttcggctgtt aatgggcgaa tcttcagtac
    15361 tggtattagg tggacaacgc gcgctgccta aacggctgga agaagcgggt tttgcgtttc
    15421 gttggtacga tttagaagag gcgctggcgg atgtcgttcg ctgatgtggt ttactgcaaa
    15481 catccgccag ttaactcccg gtgttacagg gttagtggct ttgcgcgata agatcgtctg
    15541 gtgaaagtcg ggtcaccatc agaactaact ctctgtctaa acctctatcc agcatctcct
    15601 gagcaatacg cagggcttct tcgtgtttgc cctgcattgc gccttcttca cgtaatctgt
    15661 cagcaatggt catcagtttc tccttttctt gtggtgcgcg ttctgttatc tcaccaataa
    15721 atgcacggaa acgctgggca tcccctgttt gtaatacgta attaaacagg gcttttagct
    15781 gtctgtcatt agtgttccct gtaactagca gcgaaacaat ttggtcgact aatcccaaca
    15841 gatcccgctg acgaatatgt ttctgaatta actccaacag cgccattttg cggtgttgca
    15901 taatctcgtc atccggcacc acggtaatat ccaccaacgg aaacgctgat gaatatattt
    15961 tgcgggctat agcaggctcg gcaaattcat caagccagca gagtgaataa ggataaggac
    16021 ttctgcaacc atgataaaac agcatcggga tcaccaatgg aagctcttta tagcccgcat
    16081 caagatggtt ttgcattgcc gcaatggaat aacgcatcat gcgaaaagcc attaattctt
    16141 ccggcttact ttggtgctct atcactacat aaatataacc cactccctcc tgcgttttca
    16201 cagaccacaa gaggtcggaa taatattgcc gcaggtcttc atcaataaaa ctgtttggtt
    16261 ccagtttaag cgtcgttaaa tcacacagtt tgcgcagcgg cgcgggaaga tgaatatcaa
    16321 taaaatcccg cgcggtgtct ggatggcgta aaaaagattt aaataccgca tcatgcggcg
    16381 tggaagttgt cgatattgtc atggcgttcc gtcacccctc aaatttaaga tgacgcgaca
    16441 ataaccacga ttaacaccca atgcagcagg cagtttttat ctttggacag cgaattcaga
    16501 agtattttta gcggactgca acttttacat ttcagcggaa gctgactcgc aaattcccca
    16561 tgaaaatact ccggcagcgt tagccaccgg agaggaaatt atttcagcga tcccttaagg
    16621 aactgttgca gacgagggct ttgcgggttg ccaaataact gctccggcgc gccctcttct
    16681 tctattttcc cctgatggag gaaaatgaca tgagtagaaa catggcgagc aaagcccatt
    16741 tcgtgagtca ctaccaccat ggttttcccc tcttctgcca gttgctgcat aatacgcaac
    16801 acttcgccta ccagttcagg atcgagcgcc gaggtaggtt catcaaacag taaaacttcc
    16861 ggttccatcg ccagcgcccg cgcgatagaa acacgctgtt gctgaccgcc ggaaagatgc
    16921 accggatatt tcccctgcgc acgttcgtct atcccgactt ttgccagata cttgaccgcc
    16981 cgctcgcgcg cttcctgctt gctcaggccc aacacctgaa tcggcgcttc catgacgttt
    17041 tccagcaccg tcatatggct ccagagattg aagtgctgga ataccatcgt caggcgtgtg
    17101 cgcagtaagc gcagttgatt tttatcggcg actttgagtt gaccgtcttt gtcgcgcacc
    17161 agattgatcg tctggccatt gaccacgatc gacccttcac tcggtttttc gaggaagtta
    17221 atgcagcgca gaaaggtact tttccccgat cccgacgatc cgatgatgct tattacatct
    17281 ccggcattcg cttgcagtga gacccctttc agcacttcat gttcgccgta gcgtttgtgc
    17341 aaatcgataa cgtttaattt attctcggac atcgtattct cagtgcgttg aagaaggttt
    17401 cacatgctgc aaccagcgtt tttccgctct gcgaaagagg ctgatcagga cataagagat
    17461 gattaaatag agcaccgcgg caatgccgaa ggcggtaaaa ggttgatacg tggcggcgtt
    17521 aatatcgcgg gctattttca gcagatccgg cacagtggcc gtaaacgcca gcgcagtaga
    17581 gtgcagcatc aggatcactt cgttgctgta tgccggtaac gcaatacgca gcgcagaagg
    17641 caaaataatg caacgataca ttttaaaagt cgagaagcca taggctctgg cggcttcaat
    17701 ttccccatgc ggaaccgaac ggattgcccc agcaaaaatc tcggtagtgt aagcgcaggt
    17761 gttaagcgtc agcgccagca cggtacagtt caggccactg cggaagaaag cgttaaggaa
    17821 ttcggttccc ttaacaatct caagcgtgta catgccggaa tagaacacca gcaactgaac
    17881 atacagcggc gtaccgcgaa aaatataggt aaataaccag attggaaact ggatgtattt
    17941 attactggag acacgaccaa tcgccagaaa cagcgccagg actccgccta tcactaccga
    18001 caaaataagc agccacagag tgatcgccac accagtaaag cgataaccgt cggtccacag
    18061 cagcggtttc cagtattcat gtaagatttc gatcacaggt cagccctctt cacacccacg
    18121 gagtagcggc gctcaaggaa cagcagcaca ccattggaaa cagtggtgaa aaccaggtaa
    18181 atcacgccac agacgatggc gaaatagaac ggttcccagg tacttttgcc tgccagttgg
    18241 gtggctttga ccacatcttc caggccgagt aacgaaacca gtgcggtaga tttgaggatc
    18301 acctgccagt tgttgccaat gcctggcagg gcgtaacgca tcatcgccgg aaacatgatc
    18361 cgccgaaaca cttgcccacg agtaaaaccg aacgccgtcg ccgcctctat atgccctttc
    18421 ggcactgcca taaacgcgcc acggaacgtt tcggtaaaat aagcaccgta gataaaaccg
    18481 agagtaataa taccagcgac catcggatcg atatcaatct gcccgacgcc catcgcctcc
    18541 gtcaccgtgt ttagcgcaat ctgcaaaccg tagaaaatca gcagcatcaa caccaagtcc
    18601 ggcacgccac gaatcagcgt ggtgtaccct tcgaaaatca gccccgaaag ccgattttgc
    18661 gagagcttac cgccagcgcc aattaaaccg atgattacag cgagcactac agaactgata
    18721 gccagctcca gcgtgacgag cgcaccctgt aaaataacac ctgaaaaccc atacaacatg
    18781 ctgcctgtcc tgtcgtgtgt ggtgaattac cgcctcttgt ctccctccgg ttacccgaag
    18841 ggagcgggct ttctttcacc gatggcgatt aaccgccata aacatcaaaa tcgaagtact
    18901 ttttcgctaa tttctcgtaa gtaccgtcag cgcgcatttc ggcaaaggct ttgttcagtg
    18961 cctcgcgcag ttcgttatct tctttacgca ggcccatgcc ggtccctacg ccaaacagtt
    19021 tttcatcttt aacagacggg ccaccgaatt tgtaatcttt accgacgggt tgtttgagga
    19081 aaccttcact ggcagcgacc tcatcctgga acgcggcatc aatacgtccg gcagtcaggt
    19141 cagaataaat gttgtcctgc ccctgatacg agacgatttc aatgcctttt ggtgcccagt
    19201 gttcgttacc gaacgtctcc tgggtggtgc cctgcaatac gcctacccgt ttgcctttca
    19261 gcgactcgac tgtcggctga atgtcggaat ttttcgccac caccaaacga gaatcggcag
    19321 cgtacagttt gtcggtgaag gctatttctt gctgacgttt ttccgtaatg gaaagcgatg
    19381 acatgatggc gtcaatcttc ttcgctttta aggacgggat taacgcatcc agcggatttt
    19441 cgacaaacgt acattgcgta ttgatgcgtt tgcataattc ctttgccaga tcgatatcga
    19501 agccaaccag ttcgccttgt gaattttttg attcaaatgg cgcataggtc gggtcggtac
    19561 cgatgcggat gttttgcgga atcgcagcaa atgccgcagt tgcgctggag aaggccagaa
    19621 ccagagagag cgatagcacc agttttttca taatgtcctc aactgacagt ctttttatag
    19681 ggaatattta caggtttgtt acacttatcg tgccataaaa ttgaccatca aggcaaaata
    19741 tcccgccccg ttagacgtat tatttttcat taaaaatgct taagtatgca tttcaatgca
    19801 ccacgatagt gcaacaaacc tggcgtgcac catgatgatc attccatcag gtacagcttc
    19861 ccagcgacgt atcagtcacc gtagacatta aagtcgaaat actttttcgc catcttgtcg
    19921 taggtgccgt cctgacgcag ctcgccaagc gccttattga aggcagccgt cagttcagca
    19981 tcatctttac gtagccctac accggtgcca tcaccgaagt attttttgtc ttttactgat
    20041 gagccagcaa aggcgaaatc tttaccagca ggttgcttga ggaatccttc gctggcagca
    20101 acttcatctt gtaacgcagc atccagacgt cctgcagcca gatcggaata gaccaaatcc
    20161 tggttggcat aggccaccac atccacgcct ttgctacgcc aggtctcgtt agcgtaagct
    20221 tcctgggttg atccctgcag cacaccaaca tgtttacctt tcagtgaatc cagcgttggc
    20281 tgaatcggtg aacctttggc agcaatcaaa cgagaatctg cggcgtacag cttgtcggag
    20341 aaggcaatct cctgctgacg tttatcggta atggaaagcg acgaaataat ggcgtcgatt
    20401 tttttcgctt tcagtgaggg gatcagcgcg tcaaagtcac tggcaaccca ggtacatttc
    20461 acctgcatcc gtttgcacat ctcgttaccg agatcgatat caaagccaac aaaatcacct
    20521 ttagcatctt tcgacgagaa cggcgcatag gtggtatcgg ttccgatacg taccgtctcc
    20581 ggtagcgccg catagctgga agccgctgcg gagagaccga ctaacaaaga cagagcgaga
    20641 atcgacttct tcatacataa ccctcaagtg aaaatggctt ttttatgttg tgtattgtgt
    20701 tgtgtgtttg caggctcttt catgcaggtc ttatgccatc ttgacgacaa cagtaacatt
    20761 caacgttaaa tatgttaata agacgttgca ttattgtcct gaagttgaag atagcaggta
    20821 tagcggttga atcgcagcgt ttcgattgaa tggcagaaac aaaatgtcga ggatttgatc
    20881 gcggttgcaa aattgccctg aaacagggca acagcggagt tatgcgccct gccagcgggc
    20941 aaagagatct tcaggaaggg ttatcgcaaa ctggtcaaga acacgattaa ccgtctgatt
    21001 tatcacatca tcaagggatt gcgggcgatg ataaaacgcc ggaacgggag gcataatcac
    21061 cgcaccgatt tctgccgcct gagtcattaa acgcagatgg cctaagtgca atggtgtttc
    21121 acgcacgcag agcaccaacg ggcgacgctc tttcagcacc acatctgccg cacgggtcag
    21181 taaaccatcg gtgtagctat ggacaatgcc ggaaagggtt ttgattgaac agggtaaaat
    21241 aaccatcccc agcgtctgga aagaaccgga agagatgctg gcggcaatat cgcgcgcatc
    21301 gtgcgtgaca tcggctaatg cctgcacttc gcgcagagaa aaatccgttt cgagggataa
    21361 ggtctggcgc gctgcctggc tcatcaccag atgcgtttcg atatctgtga catcgcgcag
    21421 aacctgtaat aagcgcacgc cataaatcgc gccgctggca ccgctgatgc ctacaatgag
    21481 tcgtttcata aatagttgcc ctgtcagact tgcaggcaga ctttgcagga tttcggcggg
    21541 agttgcaagt cagggtgcca gaccggcacc ctcagcgaag gcatcatcct tcgttatgca
    21601 tttcgagatt ttccacttcg ttctgacgtt gcactgcttt ggcgtcatca ttacgtaacg
    21661 tatcgaggaa atcgaggtag ccctgatcaa catctttggt gacgtagacg ccgttgaaca
    21721 ccgagcattc aaactgctgg atatccggat tttcagcgcg aacggcgtcg atcagatcgt
    21781 tcagatcctg gaaaatcaac ccgtcagcac cgatgatctg gcgaatttca tcaacttcgc
    21841 gaccgtgagc gataagctcc gtggcgctcg gcatatcaat accataaacg ttcgggaagc
    21901 gaatttccgg tgccgcagaa gcgaggtaca ctttcttcgc tccggcttcg cgtgccatct
    21961 cgataatctg ctcagaagtg gtgccacgga cgatggagtc gtcgaccagc aggacgtttt
    22021 tatcgcggaa ctcggcgcgg ttggcattca gtttacggcg cacggactta cgacgcagct
    22081 gctggcccgg catgataaag gtgcggccaa catagcggtt tttaacgaag ccctggcggt
    22141 acggtttgcc cagaatacga gcaatttcca gcgcgatatc acacgaggtt tctgggatcg
    22201 ggatcaccac gtcgatatcc agatcttccc attcgcgggc aattttctcg cccagtttcg
    22261 tgcccatatt cacacgcgcg ctgtaaacgg aaattttgtc gatgaacgag tccgggcgag
    22321 caaagtatac atactcaaac aggcacggat tgctgaccgg attgtcagca cattgacggg
    22381 taaacaactg cccttcttca gtgatgtaaa tcgcttcgcc cggcgcgacg tcacgcagga
    22441 aatcaaagcc cagcgtatcg agcgctacgc tttcggaagc gaccatatat tctgtacggt
    22501 tctcgtcaat atcacgtttt cccagtacca gcggacgaat cccgtttgga tcgcggaaag
    22561 caaccatacc gtggccgata atcatcgcca cacaggcata cgcgccgcgg attaagcggt
    22621 ttgtggcagc aatggcagcg aaaatattgt cggcttccag cgggtagtgg cggaaattgt
    22681 ccagctcgct ggcgaagata ttaagcagaa tttccgagtc ggaagtggtg ttgatgtggc
    22741 ggcgtttttc ttcaaacagt tttttacgca actcgtgagc gttggtcaga ttgccgttgt
    22801 gggcaagcgt aatgccatac ggggagttaa cgtaaaacgg ctgcgcttca gaggcgctgg
    22861 agctgccagc cgtagggtaa cgcacatgac caatgcccat attgccctgc aaacgctgca
    22921 tatggcgagc ttcaaataca tcgctcacca gcccgttcgc tttacgcaaa cggaagcagt
    22981 tattggcatc tatggtgatg atgccggcgg catcctgacc gcgatgctga agcaccgtta
    23041 aggcatcata aatcgactgg ttaaccggca taacaccggc gataccgaca ataccgcaca
    23101 tacgtctttt tcctcgttaa gccacatctc agagcactta cgctctgggc aagaaacttg
    23161 acgagctttg cagataatca aaaaaccatc tgatgataaa actgaattgc gggatcagct
    23221 gtgatttgct ccagtcttcg cttttcgaca ccccggtaaa ggagtcgaga aagaagagaa
    23281 tggcggcaac aatcaacaca ccgcgcaacg caccgaaaca gacgcccagc acccgatcgg
    23341 tgcctgacaa ccccgttttc tccaccaact ggcctatcac gaagttcacg atagcaccaa
    23401 cgatcagggt agcgataaac agtaccgcga tggcaatccc atttcgaacc agttcgtctt
    23461 caaagcccgt aaaccagact gacaggtaag tgtagtaatg actggcaaca aagaaagcac
    23521 aaccccatgt caccagcgat aacgcttcac gaacaaagcc gcggatcagg ctaaccagag
    23581 aggaaaaagc aatcaccgcg attatggcgt aatcaatcca gaccatatgt gtcccacgat
    23641 tttacgccct gtcatcctgt tcggggcgca ttctaacaga aaaagaaaac gtttgcgtag
    23701 ggatttcctt cccacgcatc aataaaaatg gcgctgaaaa aatattcaac gccatcgact
    23761 ttttatgcct ttgcggcatc gtgcaatgcg tgtcggatgc ggcgtaaacg ccttatccga
    23821 cctacggttc taacactgcc caggcctgat aagacgcgcc agcgtcgcat caggcaagac
    23881 tgtattaatt cggcgtatag cccattacca cgccacttaa gccagaaagt tgcttcaact
    23941 cacccagcga acctttcagc ttatctttcg aggcatccgg cccaaccaga atacgggtaa
    24001 ttttaccctg cactggcgtg gatggcgacg tataaacccg ataaccggca ccgcgcagct
    24061 taccgacaat ctcattcact ttatcggcat ttttcagcgc acccagttgc acaacatagg
    24121 ctttacccgt cggtgcagct ttttcttcca cgaccggctt cggttctggc ttcggcgcag
    24181 gtggtgcttc aacctttggt ttaggcggct ccaccggttt cggcttcggt ggggcgaccg
    24241 gtgcaggttc cggttcaaac tcggtgttat tggctgcaat agtggccgga tcgagcgacg
    24301 gtgctgcggc atcacctgcc cgcacctctt ccgctgcgcc ttccggcggc tgcgtcggca
    24361 acgcctgggt ggcggctggc atcatatcag gctcatcgcg atcgcccgct tttggaacca
    24421 gcgggatagc cgcaaactca tcctgataat gttttttctg cccgtccagc agccctggaa
    24481 gtacaatcac ccccagcgcc accagcacga tcgtgcccac taaccgattc tgaaacttac
    24541 ttgccaccgc ttctcctcgc gtcaatcact tccatgacat gtgcgaccgt gtggaaagaa
    24601 ccacacacca gcacggtgtc ttccgcttta gcgtccgcca ttgcggcatc ccatgcctgc
    24661 gcaacgctat caaatgattt gccgttaccc aaatgctcaa gcagttgttc tgccgtggca
    24721 ccgcgcggcc cttccagtgg cgcacaatac cagtcatcaa ccacgctttt caaccaggcc
    24781 agagttccgg caatatcttt atcatgtagc ataccgataa ccgccagcac gcgcccgttt
    24841 ttcggtaagg ctttcatacg cccggtgaga tattccgccg catgtggatt atgcgcgaca
    24901 tcaaaaataa cgcgtggcga ctcgctcaca atctggaaac gtcccggcaa aattgcgctg
    24961 gcaatcccgt cgcgaatggc attttcactg acctccagcc cgctggcacg cagtgccgcc
    25021 agcgccgttg cggcattcgg ttgcgggaca agcggcaacg gcaaattttc cagcgtgccg
    25081 tgagcatcgc taaacgtcca gtcatgatcg gtgacggaat agttccactc aacgccccga
    25141 cgttgtaaca gtgcaccttt ttcctgcgcc acatcagcaa tggtagaagg catttccggc
    25201 tcaccgacaa ttgccggttt ttcgctgcga aagatgcctg ctttctcgcg accaatactt
    25261 tcgcgatctg gccccagcca gtcggtatga tccagcgcaa tgctggttac taccgcgaca
    25321 tcggcgtcga caatattggt tgcgtccaga cgaccgccca gccctacttc cagaatcacc
    25381 acgtcaagtt gtgcctgttt gaacagccac aacgccgaca gcgtaccgta ctcgaaatag
    25441 gtcagggaaa tatcaccgcg tgccgattca atctccgcaa aagaggcggt gtgggccgat
    25501 tccggcaatt cctgaccctg cacacgtacg cgctcggtat aacgcaccag atgaggcgaa
    25561 ctgtagacgc ccactttgta ccctgccgcc atcagaatcg actccagcgt acggcaggtg
    25621 gtgcctttgc cattcgtacc cgcaacggta aacacaaatg gcgctggttt cagaacgcca
    25681 agacgcgccg cgacctggct cacgcgctca aggccgagat cgatagtttt actgtgcagg
    25741 ttttccagat aagaaagcca cgaagccaga ggcgacgcgg cttgaggagt gcgtttgata
    25801 atcatggtat ccgctgattc gttacggtga gaatagcaaa agggcagagc cagtggccct
    25861 gcccttatca gttatcaggc ctcaggttcc tgatccggta ccgggggtac cactacgcct
    25921 tcacgcggcg cttcaggatt cggcgctggc agattcatca acttcgccag aatgctcgcc
    25981 agtttcaggc gcatttccgg acgacggacg atcatgtcga tcgcgccttt ctcgatcagg
    26041 aattcactgc gctggaatcc tggcggcagt ttttcgcgaa cggtctgttc gataacacgc
    26101 ggaccggcaa agccgattaa cgctttcggt tcagcgatgt tgagatcgcc cagcatggcg
    26161 aaacttgcag aaacaccgcc catcgtcggg tcggtcagca cggagatgta cggcaagccg
    26221 cgctcctgca tttttgccag tgccgcagag gttttcgcca tctgcatcag cgacatcagt
    26281 gcttcctgca tacgtgcgcc accagaggcg gagaagcaga tcagcgggca gttatcttcc
    26341 agcgcctgct caacggcacg cacgaaacgt gcacccacaa cagaccccat tgaaccgccc
    26401 ataaaggcga actcgaatgc cgcagcgaca accggcattc catacagagt gcctttcatc
    26461 accaccagcg catctttttc gccagtttct ttctgcgcag atgccagacg gtctttatac
    26521 ttcttggagt cacgaaactt cagcacatct ttcggctcaa gctcgctacc cagctccaca
    26581 aggcttcctt catctaacag gctatgcagg cgattacgcg ctgtcatacg catgtgatgg
    26641 tcacacttcg gacagacctc aagattacgt tccagctcag cgcggtataa aacctgaccg
    26701 cagctatcac acttagtcca caccccttca ggaatgctcg ccttgcgggt gggagtaatg
    26761 ttgcttttaa ttcgttcaat ccagctcatt agggaccttt ctgtctgaac ctggttcgat
    26821 gccagtttta tctttgggga cgcataatgc catttttgcc cccaacagac catgaatgtt
    26881 gcacattaaa acataacagc ccgaaacttt ggataaaaaa gtggtcgaac cgcggagtta
    26941 ctttttattt tgcggcgcgt gccgcagcgc gtttgtgacg aataaattcg aaaaccccag
    27001 gcaatattga aacaaaaata atgccaacaa tcatcagctt aaggttgctc tgaatgaagg
    27061 ggagcgtgcc gaagaaataa cccgcgtagg taaaaatcag tacccacaac agtgcgccga
    27121 tcacgttata agcggcgaaa tgacggtacg acatgtggcc cattcccgca acaaacggcg
    27181 cgaacgttct gacgatcggt acaaaacggg cgagaataat cgttttgccg ccatgtctct
    27241 cgtaaaactg atgagtctta tcaagataac tgcgacgaaa gattttcgaa tccgggttac
    27301 tgaacagttt gtcaccaaat aaccgtccaa tggtatagtt cactgcatca ccgacaatag
    27361 cggcaatcaa cataagcacc accatcatat ggacattgag atcgttagtt tccagcgacg
    27421 ccaatgcacc agcaacaaac agcagcgaat caccaggcag gaatggcgtt accaccagcc
    27481 ccgtttcgca gaacagaatc aaaaacagaa tggcataaac ccagacgccg tactccgcga
    27541 ccagctctgc cagatgcacg tcaatgtgca ggataaaatc gatgaggaaa taaatcaggt
    27601 ccatatttgc ctatgccttg tactcgtcat ctttcaggct gtaactacgt tggctacagc
    27661 ctgaaatgtt ccgagcatta ttctcgttag tccgccagaa atagcgggcc cattggcggt
    27721 tttggaagat cataccggtc agggtaatcc accgcgacca gatacagccc ttccgctttt
    27781 gccgttgctg ccgccagcgt tctgtccttt gccgccagca actctgctat ccagctctcc
    27841 ggctggttgt gggcaccgac ttccatcagg ctgccgacaa tattcctgac catatgatgt
    27901 acaaaggcat tcgctttgat atctaccacc acataaggac cgtgacgcgt gacattaata
    27961 tgcataacgt tacgccacgg ggttcgggac tggcactgca ccgcacggaa cgaggtgaaa
    28021 tcattctcgc ccagcaagca ttgcgcagcc cgatgcatcc gttcagcgtc cagcggttcg
    28081 taaaaatggg ttaccccttt actcagtacc gccgggcgca gccgatgatt gtagatgatg
    28141 tagcgataac ggcgagccgt ggcgctaaat cgggcatgaa aatcatcagg tacagcttta
    28201 acccaacgca cggcgatgtc accaggtaaa ttcgcattta cgcccaaggt ccacgccgcg
    28261 tctttgcgct gcgcggttgt ttcgaaatgc accacttgcc cggtaccgtg tacccctgcg
    28321 tcagtacgcc cggcgcagaa gacggtgatg ggttcgttcg ccacctggga gagcgccttt
    28381 tccagcttct cctgcacact gcggacttca ttctgccgtt gccagccgta atacttactg
    28441 ccgtcgtact caatgcccag cgcaatttta taaactggca gttgttgctg gtcggacatt
    28501 agtacagata ctcctgcacc agtttctcgg cgatttttac tgccatcagc gcgccgccaa
    28561 agcgaacgtt atcggccacc gaccagaact ggacttgctc cggcataccg tagtcattac
    28621 gcacgcagcc aacagaaaga tgcggcgtac ccgaagcatc ccctacctga gtcgggaatt
    28681 cgttctcttc agagagcaca atatcttcgc cctgagcaaa cgcatcacgc gcttcttctg
    28741 ccgccagcgg acgcagtgct tcaaagttga ccatctgggc atgaccgtag aataccggtg
    28801 cctggacgac gcttgccgaa atcatcagcc cttcgtcctg caggattttg cgtacttcgt
    28861 caacgatacg acgttcttca cgcacgctac cttcgctatc cggcaacaac ggcagcatat
    28921 tgaacgccag ttgacgaccg aagaaatctt cttcgtcaat cggaatgccg ttgagcaatt
    28981 tcgcactctg ccccgctaac gcatcgaccg cttttttgcc ctgggcggag gctgaaatca
    29041 ggctggtaac actgatacgc gacaaaccgc cctgatcgat taacggtttc agtgccgcca
    29101 gcagctggct ggtcagactg tctggtacgg cgatgacatt ccggttgcgg taatcggtca
    29161 gtacaaacgg gtttacttcc ggcaccacca gcggtacgtc gggttcgaga gcaaacagtc
    29221 cgctgctgtc gatcaccagg caacctgagt tggtcgcttc ttcaacccag gcagcggtag
    29281 cttctttgcc tgcgacaaaa aatgccagct gcgcctgcgt ccagtcgaat tcagcggcat
    29341 cctgcacggt gattgtttta ccaccaaagc gcagttgttc gcctgcgctt tcgttacgtg
    29401 ccagtgcata aatttcccca accgggaact gacgttcagc cagcgtttca agcagggctt
    29461 cgcccacagc gccagttgcg cccaggacgg caatgttcca gccttcagac atggtggttt
    29521 actccagaaa tagcaaagct ccctgccaat gttacagcag agagcatgaa gaagagatta
    29581 acgtgccgga tgatgaacgg cgttaaaacc cagtttacac agcaatgatg ccgcactggc
    29641 gtcatcacaa attacataca gagatgacca ttcacggcgc tcaagatagt ttttgcgcag
    29701 tttatcgaac tcacccggta tcccggcgac tttacgcagc ggtgcgtcat cgcggcgcac
    29761 atcatacacc aaatgcacca gccttttcag cgtcggttga tcgagcgggc catgcagcgt
    29821 aatgcgacca aactctggcg cgggcagtaa tgtatccagc gcaacgtgct gttcatgccc
    29881 aataaacttg ctataggctt caaacacttg cgtagtaccg cgtgctttac cttccagggt
    29941 ataacctgcg atatgcggcg tgccgatatc cacttttgtc agcaactcca cgttaagttc
    30001 cggttcgcct tcccagacat ccagcactac gcttaacttc tggccttcat tcaggcaggt
    30061 cagcaacgca gtattatcga cgactgcgcc acggcaggcg ttaatcagaa tcgctcccgg
    30121 cttcaggcta cggatcagtt tttcatccgc cagatgcagc gttttgtacg gcccatcttt
    30181 gaagagtggc gtatggaaag tcagaatatc cgcatgctga actaattcat ccagcgagcg
    30241 gaaatcaccc tcatccccac ggtcagcgcg aggcggatcg caaagcaatg ttttaatccc
    30301 taacgcttcc agtcgcgcct gcaatcgacg tccaacgtta ccaacgccca cgatccccac
    30361 cgtacggtcg tgcagtgaaa atccatcgcg ttcggcaagc atcagtagtg aggagaaaac
    30421 atattccacc accgcaatcg cattacagcc aggtgcagcg gaaaaaccaa ttcccgcctg
    30481 cttcaaccat gcttcatcga catggtcggt ccccgcagtg gcagtgccaa caaatttaat
    30541 gggttttcct gccagcaaag attcattcac tttcgtgacc gaacgcacca tcagcgcatc
    30601 cgcgtctgcc agttgagcga cggggattgg acgcccggga accgcggtca cctcacccaa
    30661 acggctaaat aagtcgcggg cataaggcat attttcatca acaaggattt tcacgtttgt
    30721 gttacctgta tgagacgaga gttaaccgga caagtgtgcc ataatctcgc ggccaggcat
    30781 acttgcgaag atttcaggta taaggatacg taatgataca acctatttcc ggccctcctc
    30841 ctgggcaacc accaggtcag ggagataacc tgccgtctgg cgcgggcaat cagcctttat
    30901 ccagtcagca acgtacttcg ctggaaagct taatgacgaa agtgacctca ctgacgcaac
    30961 agcaaagagc agaactgtgg gcgggtatca ggcacgatat tggtctgtcg ggagattcac
    31021 cgctgctttc gcgtcacttc cctgccgctg agcataatct ggcgcaacgt ctgctggccg
    31081 cgcaaaaaag ccattctgcc cgccagcttt tagcgcaatt aggggagtat ttacgtctgg
    31141 ggaataatcg tcaggcggtc acggattata tccgtcataa ctttggtcag acgccgctga
    31201 atcagctctc accggagcaa ttaaaaacca ttctcaccct gttgcaggaa gggaagatgg
    31261 ttattccgca accacagcag cgcgaggcga ccgaccgtcc tttattaccg gcggagcaca
    31321 atgcgctaaa acagctggtg accaaacttg cggcggcaac gggggaaccc agcaaacaga
    31381 tctggcaatc gatgctggaa ctttccgggg tgaaagatgg cgagttaatt ccagcgaaac
    31441 tgtttaacca tctggtgacc tggctacagg cgcgtcagac gctaagccag caaaatacgc
    31501 cgacgctgga atcactacag atgacgctaa aacaaccttt agatgccagt gaactggcgg
    31561 cgttatcggc atatatccag caaaaatatg gtctttctgc gcaatcatcg ctttcttctg
    31621 cccaggccga ggatattctt aatcagcttt atcaacggcg ggttaaaggg attgatccgc
    31681 gtgttatgca accgctgctt aatccttttc caccgatgat ggacacgttg caaaatatgg
    31741 caacgcgtcc cgcgctgtgg atactgttag tcgcgattat cctgatgctg gtctggctgg
    31801 ttcgttaacc ccgacgaaat gacagtatcg tgacaataat acccagcacc gcagagatcg
    31861 ccccggcaag aaataccgaa gagtaaccaa acgtggtcgc cagcattccc gccagcggcc
    31921 cggagacgcc gagggcgata tcctgaaacg cggcgtaacc gcccagtgcg gtgccgcgaa
    31981 cttgtgaggg gacgcgttta accacctcca cgcccagcgc aggaaagata agcgaacatc
    32041 cggctccggt taacgccgcg cccgctaatg cgacccatgc acctggggct tgccagagca
    32101 gcaacaagcc caccgtttct acaagcagag agacaatcgc cactttcacg ccgccaaaac
    32161 ggtccggcat ccagccaaac atgacgcgca tcacgacaaa tgcgccgcca aacgcggtaa
    32221 gagtaaagcc cgccatcgcc catcctttgc tggcaaagta gagcgaaacg aaagtcccga
    32281 taacagcaaa accaacgcct tgtagtgcca gacctaaccc tggtttccag ataagcccga
    32341 caacgctcca cagcgatgga cgttctcccg ccagggccgg tactttgcgc actgtgccgt
    32401 tacaggccca cgccagtacg ggtaatacca ttgtggtgat cgccagtgcg gcaaaaccgt
    32461 aatggctatg aatcaacagg ccaagcggag caccaacagc gagggcaccg taaatcgcca
    32521 ttccgttcca tgacatcact ttgccagagt gttttggccc tacgatgcct agtccccagg
    32581 tcagagcgcc tgtcagtaac tggctttcac caaagccaag aatcaaacgc ccgacgacca
    32641 acagagcaaa tttgaacggt gcggagacag gcaaaatcgc cgccagcagc aacgcgccgc
    32701 cagccagacc acaagctaac attccctgaa gcgccgaacg ttttgcacca tattgatcgg
    32761 ccagtcgccc ggcgtaacca cgcgtcagca ccgtagccag aaactgaatc ccgacggcaa
    32821 tgccgaccat ggtattgcca tagcccagtt catgatgaac aaacagcggg ataaccggca
    32881 acggcaaccc tacggtcatg taggtgagaa aaaccgcaaa agcgatgcgg aagagcgaaa
    32941 aattggcaga agatcgtgtt tcggtttggc ttacagcagt catgcattac tccagaatgc
    33001 agcgcaaggc gaggagtatc cccgtctcat ctctctggtt tcagggttac ggtgcgttgg
    33061 caggatttaa cgcgtacgtc ttttcagaag gaaatcgaca aagcgggaag tttgcctgga
    33121 actggcggcg attgtcaatg atgtgaaaaa gggaaccatc aggttccctt ttgcgttagt
    33181 gccggaggcc gcattggcgc gtaacgtcgg atgcgacgct ggcgcgtcta ctccgaccta
    33241 ctgcgaatta atctttcagc ttgcgcatta ccagcgtggc gttggtgccg ccgaagccga
    33301 agctgttaga cataacggtg gtcagttcgc gatcggtcgt ttcggtcacg atgttcagac
    33361 ccgcagcctg ctcgtccagc tcttcaatgt tgatgctcgg ggcgataaag ccgtgttcca
    33421 gcatcagcag agagtagata gcttcctgta cgccagcagc gcccagagag tgaccggtca
    33481 tggctttggt tgcagaaatc gccgggctct tatcgccgaa cacttcacgg atagctgcca
    33541 gctctttcac gtcgccaacc ggagtcgaag taccgtggga gttcaggtaa tcgattgggg
    33601 tatcaacgcc atgcatcgcc atcttcatgc agcgtactgc gccttcgcca gacggagcaa
    33661 ccatgtctgc accatcagag gttgcgccgt agccaacgat ttcagcatag atgtgagcac
    33721 cacgcgccag cgcgtgttcc agctcttcaa ccactaccat accgccgccg ccagcgataa
    33781 cgaaaccgtc acggtgagcg tcgtaagtac gggaggcttt ttccggggtg tcgttgtatt
    33841 tagtagacag cgcacccatt gcgtcgaatt cgcaagccat ttcccagcac agctcttcgc
    33901 cgccgccagc aaacacgatg tcctgtttgc ccagttggat ctgctctact gcgttaccga
    33961 tacagtgtgc ggaagtcgca cacgcggagc tgatggagta gttaacgcca tgaattttaa
    34021 acggggtggc gaggcaggca gaaacgccgg atgccatcgc tttggtgacc acatacgggc
    34081 caaccgcttt caggccgcgc gggccgcgca ttgcgtcagc gccgaacacc tggaaacgcg
    34141 gggagccgcc gccggaacct gcaatcaggc caacgcgcgg gttattctgg taagcttccg
    34201 gagagaggcc cgcatcagcg attgcctgct ccatagaaag gaatgcataa atggatgcgt
    34261 cgctcataaa gcgcacaact ttgcggtcaa tgaggccagt ggtatccagt tttacgttgc
    34321 cccagacgtg gctacgcatg ccggaatcct tcagctcctg agagaaagtg atccctgaac
    34381 gtccttcacg cagagatgcc aggacttcct gctggttatt accgatgctg gaaacaatgc
    34441 ccaggccagt aatcactgca cgtttcattc aatacctctg taagtcgcac atagagtaag
    34501 tttcgaatgc acaatagcgt acacttgtac gccgaacaag tccgatcagc catttaatag
    34561 agaaatttgc gcagccttac acacatcgct aagatcgagc caccgcctgt aagacgagta
    34621 acttacgtga aacactactc catacaacct gccaacctcg aatttaatgc tgagggtaca
    34681 cctgtttccc gagattttga cgatgtctat ttttccaacg ataacgggct ggaagagacg
    34741 cgttatgttt ttctgggagg caaccaatta gaggtacgct ttcctgagca tccacatcct
    34801 ctgtttgtgg tagcagagag cggcttcggc accggattaa acttcctgac gctatggcag
    34861 gcatttgatc agtttcgcga agcgcatccg caagcgcaat tacaacgctt acatttcatt
    34921 agttttgaga aatttcccct cacccgtgcg gatttagcct tagcgcatca acactggccg
    34981 gaactggctc cgtgggcaga acaacttcag gcgcagtggc caatgccctt gcccggttgc
    35041 catcgtttat tgctcgatgc aggccgcgtg acgctggatt tatggtttgg cgatattaac
    35101 gaactgacca gccaactgga cgattcgcta aatcaaaaag tagatgcctg gtttctggac
    35161 ggctttgcgc cagcgaaaaa cccggatatg tggacgcaaa atctgtttaa cgccatggca
    35221 aggttggcgc gtccgggcgg cacgctggcg acatttacgt ctgccggttt tgtccgccgc
    35281 ggtttgcagg acgccggatt cacgatgcaa aaacgtaagg gctttgggcg caaacgggaa
    35341 atgctttgcg gggtgatgga acagacatta ccgctcccct gctccgcgcc gtggtttaac
    35401 cgcacgggca gcagcaaacg ggaagcggcg attatcggcg gtggtattgc cagcgcgttg
    35461 ttgtcgctgg cgctattacg gcgcggctgg caggtaacgc tttattgcgc ggatgaggcc
    35521 cccgcactgg gtgcttccgg caatcgccag ggggcgctgt atccgttatt aagcaaacac
    35581 gatgaggcgc taaaccgctt tttctctaat gcgtttactt ttgctcgtcg gttttacgac
    35641 caattacccg ttaaatttga tcatgactgg tgcggcgtca cgcagttagg ctgggatgag
    35701 aaaagccagc ataaaatcgc acagatgttg tcaatggatt tacccgcaga actggctgta
    35761 gccgttgagg caaatgcggt tgaacaaatt acgggcgttg cgacaaattg cagcggcatt
    35821 acttatcctc aaggtggttg gctgtgccca gcagaactga cccgtaatgt gctggaactg
    35881 gcgcaacagc agggtttgca gatttattat caatatcagt tacagaattt atcccgtaag
    35941 gatgactgtt ggttgttgaa ttttgcagga gatcagcaag caacacacag cgtagtggta
    36001 ctggcgaacg ggcatcaaat cagccgtttc agccaaacgt cgactctccc ggtgtattcg
    36061 gttgccgggc aggtcagcca tattccgaca acgccggaac tggcagagct gaagcaggtg
    36121 ctgtgctatg acggttatct cacgccacaa aatccggcga atcaacatca ttgtattggt
    36181 gccagttatc atcgcggcag cgaagatacg gcgtacagtg aggacgatca gcagcagaat
    36241 cgccagcggt tgattgattg tttcccgcag gcacagtggg caaaagaggt tgatgtcagt
    36301 gataaagagg cgcgctgcgg tgtgcgttgt gccacccgcg atcatctgcc aatggtaggc
    36361 aatgttcccg attatgaggc aacactcgtg gaatatgcgt cgttggcgga gcagaaagat
    36421 gaggcggtaa gcgcgccggt ttttgacgat ctctttatgt ttgcggcttt aggttctcgc
    36481 ggtttgtgtt ctgccccgct gtgtgccgag attctggcgg cgcagatgag cgacgaaccg
    36541 attccgatgg atgccagtac gctggcggcg ttaaacccga atcggttatg ggtgcggaaa
    36601 ttgttgaagg gtaaagcggt taaggcgggg taatctgctc tcgcagtgtt tgccggatgc
    36661 ggcgtgaacg ccttatccga cctacgtgtg acgtgtaggc ctgataagac gcgataagcg
    36721 tcgcatcagg catggtgctc cagacgccgc aacttactgc tgtgacgcct gctgaaacag
    36781 gttttcccac atatcggtca ccagcgcctg gtcacgcggc gacaattcac cggcaccaat
    36841 ggctttttcc agactctggc taacggtcgt atgcaccgcc tgagcggagt ggtcatcacc
    36901 actttccagt tctgcgatgg ctaatgtcag gtggccacgc aaatacccac tggcaaacaa
    36961 ctcatcatca ctggcatggt caaccatatc gtcgattaat gccagaatgc gtgattcaaa
    37021 ctccgcgatc atcttctttc ctcattgtaa aacgtggcgc tggctttagt tgagcgcttc
    37081 cggccacggg aactgttccg ccgtaagttc cggcgtgtga taataatttt gtagtgcttt
    37141 aatgaagcgt gccgggcgtt caggaatgcc gtttgctagg taatccatca cctgcgcatg
    37201 tacccgccgt tgaaacacca cacgatccgg ttcgaagtcg ccttccagat tgtcgcaact
    37261 gacgttaaac ggatatcccg ccgccacaca gaacaaccag tcgaacgcct gcggcttcac
    37321 ttcaacatct tcaaactggc tttgcgtttg ggcatcgcgt ccgtccgggc aataccagta
    37381 gccgaaatca accagctcac ggcgcgcttt cccggcaata caccagtgcg aaatctcatg
    37441 aattgcgctg gcataaaagc catgagcaaa gacgattcgg ttatacggta cttccgcatc
    37501 agcaggaaga tagatcggtt cgtcgtcgcc tttaatcaga cgggtattaa attcatcggc
    37561 aaagcagcta ttaaaaattt caatcaactg ctcatagtgg tgtgtactgt tcattagttc
    37621 atccccaacc agtggaggat ctcctgtccg tggctatcaa aaagtaattt ggcactcatc
    37681 accgctgaga caataacaat catcgggcgg atcagctttt gtcctttgct caacaccagt
    37741 cgtgaaccca tgcgcgcgcc gaggaactgc cccaccagca tgacaaagcc cgtcgcccaa
    37801 atcactttgc caccgagaat aaacagcagt aaaccgccga tgtttgacgt tgcgttgagt
    37861 aatttggcgt gcgccgtggc tttggcgagg ttgaatccgc acagcgtaac aaaggccagt
    37921 gcgtaaaacg atccggctgc cggaccaaag aatccgtcat aaaaaccgac gcagccaccg
    37981 gcaattaacg cgaagggtaa accgtacatc cgacgctggc gatcttcttc acccagcttt
    38041 ggcatcagca agaaatagag gccgatacaa attaccagaa tgggcaaaat ctgccgtaag
    38101 acatcagcct gaacgtattg caccagcagt gcgccgctca ttgagccgac aaaggtcatg
    38161 gcgatattga gtttctgatc gcttaaacta accactttgc ggcgaataaa gtagatagtg
    38221 gcggaaatag agccgccgca ggcttgcagt ttattggttg ccagcgcgtt agcgggagac
    38281 attcccgcag ccatcaatgc cggaatggtg agtaacccac cgccaccggc aatcgagtcg
    38341 ataaatcccg ccagcatggc gacaaaaaag agaactccca gcaacagcgg ggaaaccata
    38401 aacaggctat taaacgtttc cattagatca cgtgctcatc cagtagcgcc tggcaggaag
    38461 gcggcaacgg aggcggtgtc ttcttctcag gctttgttgt tcccggtttt ggaggtgcaa
    38521 accagctttg cagttctgcc ccgcaaccat cgcctggtgg cggtaaaggt tgatcttcac
    38581 actccagact atcggcagga caacgtaatc gtacatgcat atgcgcgcga tgctggaacc
    38641 agggtcgcac tttgcgcaac cagtcgcgat cggtgcccgc atcaaggcaa agttgttgtt
    38701 taatcgccgg attaacaaaa atgcgtgtga cgtctttgtc ctgggcggcg agtttgatca
    38761 agctgaaaat ttctggcttc cacagcgtgg agacaacgtg tttaccgtcg cgggaaacta
    38821 agtccagtgc ttgcgggcgc aagagctgcg cggaggtcca gcgagttttc ggcagttgca
    38881 gaaagatatc gacatccagt ccggtctggt ggctggcatg accgccgttg aaacgcccac
    38941 cagcgggcat ccccatatcg ccaatcagca ccgtacccat gcccagattg ctcacctggc
    39001 tactcagacg ctggataaac atcaccagat ccgggtgacc gaaatagcga cgctgatcgg
    39061 tacgcatgac ctgataatgt tcggactgta tcggcagcgt gtcagcgccg acgatacagc
    39121 cattagaaaa actgcctatc gattgtgcgc tacccggcac aggttgggtt attttttgcc
    39181 acggcgtcgc tgccaggcta gcgctactgg caagcagagc cagcagcgca atcgcggttt
    39241 tgttcatttt ttaccagcgt ggaatatcag tcttcacatc ggcattttgc gcccgctgcc
    39301 gtaacaggtg atccattaaa acgatcgcca gcatcgcttc agcgatcggc actgcacgga
    39361 tcccgacaca gggatcgtga cggcctttgg tgatcatctc aacttcttcg ccaaagcggt
    39421 taatagtgcg ccccggcacg gtaatgctgg aggtcggttt cagcgccata tgagcaatga
    39481 tttgctgccc gctgctgata ccgccgagaa tgccgcccgc atggttgctc tggaaaccgt
    39541 ctttggtgat ttcgtcgcgg ttctggctgc cgcgcagcgc cactacgtca aaaccgtcgc
    39601 caatttccac gcctttcacc gcgttgatgc tcatcagcgc atgggcgatg tcggcatcca
    39661 ggcggtcaaa gaccggctcg ccaagtccgg cggggacacc actggcaaca acggtgactt
    39721 tcgcgccgat ggagtcgccc tcttttttca gcgcgcgcat cagttcatct aacgcgtcga
    39781 ttttgtcggg gtccgggcag aagaacggat tttgttcaac cagcgaccag tctttgattt
    39841 ccagcggaat gtcgcccatc tgggtcaagc agccgcggat ttcaatacca aatttctcgg
    39901 cgagatattt tttggcaatc gcccctgccg ccacgcgcat ggcggtttcg cgggcggaag
    39961 aacgtccacc gccgcgataa tcgcgcagac cgtatttttg ttcgtaggtg taatcagcat
    40021 ggcctgggcg gaaaacgtct ttaattgcgc tgtaatcctg agagcgctgg tcagtgtttt
    40081 cgatcaacaa gccaatgctg gtgccagtag taacgccttc aaaaacaccg gagagaattt
    40141 tgacctgatc cggctcgcgg cgctgggtgg tatagcgcga tgtcccaggg cgacgacggt
    40201 cgaggtcatg ttgcaggtcc gcttccgtca gcggaatgcc tggcggaaca ccatcgacga
    40261 tgcagccgag cgccagccca tgcgattcgc cgaaggtggt tacgcgaaag agttgtccaa
    40321 ttgtgtttcc agccatcacg gctccgttat tgttgtgttt gcgtgtttac ttaatcttta
    40381 taaatcgcga aatattcacg ggccgccagc agctgctctt tggtgagcat aaacacacca
    40441 tcgccgccgt tatcaaactc cagccaggtg aacggaacat ccggatattg ttccataaga
    40501 tgtaccatgc tgttgccgac ttcacaaatc aacacgccat catcagcaag gtaatctgcc
    40561 gcgtttccaa gaatgcgacg tgtcagttta aggccgtcgg tgccagatgc caggcccagt
    40621 tccggctcgt gacggtattc gtttggcagg tcggacatat cttccgcgtc gacatacggc
    40681 gggttagtga caatcagatc gtattgcact ttcggcaaat cacggaacag atcggaacga
    40741 atcggaatga cgttgtggat cagaccgtgt tcttcgatgt tctgttcggc aaccgccagc
    40801 gcgtcgggag agatatctac cgcgtcgact tccgcttccg ggaaggcata agcacaggca
    40861 atggcgatgc agccgctacc ggtacacata tctaaaatat gctgcggttg cttgctgatg
    40921 agtccggcaa atttattgtt gatcagttca ccaatcggcg agcgcggcac cagcacgcgt
    40981 tcatcgacgt aaaattcatg accgcagaac caggctttgt tggtcaggta agccaccgga
    41041 atgcgctcgt tgacgcggcg gatcacgcgt tcaacaatac ggtgtttttc gctggaggtc
    41101 agacgcgcgg tgcgcatatc ttccgggata tccagcggca ggtagagcga tggtaacacc
    41161 agctgtacgg cttcatccca cgggttatcg gtgccgtgac cgtaccagat atttgccgcg
    41221 ctgaagcggc ttaccgacca gcgcaacatg tcctgaatgg tttgcagctc atttactgct
    41281 tcatcaacga aaattttatc cacgtattcc tccagggcat gatcgcaata atttcggcgg
    41341 ctagtttgcc atgaagatga cgataaatca gcattcacgc gcggtgagtg aggaaaaata
    41401 cgtttaaaac gatcgattgt gctacgagtc gggtaaactg taggaaaatt agaaatagag
    41461 acagataaat gaaaaagaaa acaacactta gcgaggagga ccaggctctg tttcgccagt
    41521 tgatggcggg gactcgcaag attaagcagg acacgattgt ccaccgaccg cagcgtaaaa
    41581 aaatcagcga agtgccggtg aaacgcttga tccaggagca ggctgatgcc agccattatt
    41641 tctccgatga gtttcagccg ttattaaata ccgaaggtcc ggtgaaatat gttcgcccgg
    41701 atgtcagcca ttttgaggcg aagaaactgc gccgcggcga ttattcaccg gaattgtttt
    41761 tggatttaca cggtctgacg cagctgcagg ccaagcagga actgggcgcg ttgattgccg
    41821 cctgtcgccg cgaacatgtg ttttgtgcct gcgtgatgca tgggcacggg aagcatattt
    41881 tgaagcaaca aacaccgctg tggctggcac aacatccgca tgtgatggca tttcaccagg
    41941 caccgaaaga gtacggcggt gatgcggctt tgttggtgtt gattgaagtg gaagagtggt
    42001 tgccgccgga gttgccctga ggagttgagc gtgttgtgcc aggagcgcgg aatggcaggg
    42061 gcgaatgacc ctgccaggtg agaaaaggtg cgctaataca tattcagtat gtaagtgaat
    42121 cggcaggtag aatctatggg ctgtcgagat gtcgtgtcca cgcgtagctt catatcatgc
    42181 agttgcctgg acgtaatcgg ttgcggctca ggcgtcaccg tcactttggc attatcacag
    42241 ccggaagtcg agaaggtcat ctgctgtgca ataaaacggc agtaggcacc acgctcatca
    42301 aaattattcc cgttacacag tttgccattt tccatcagga aagtgggcag tgtttgctca
    42361 ataacaccac ctgttgactg caacctgacg actttgttat gtacaacata attttcattc
    42421 caggaaacat catacggtgc caggggcacc gccggacatg aagaactcac cttcgttggg
    42481 ccaatatagt caggaaaatc atcatcatac gttttagcga agggatctct actcttaaca
    42541 aacgacgaca caatccacgg acaaccatgt aaattcccat cgtaataaac aaacatcttt
    42601 aattcattac atgtccatcc cgttttggtc tggctatcat actgctctaa tccaggacag
    42661 gctgacatgg ataccctgct gtccgaaatt ttcgacatag atggttgagt agaactatcg
    42721 ccagtccacg aaactaaatc cggctgtgaa ttgggcatga atgtagcctt aaatatagcg
    42781 taattaactg aatctggcgt cgctataatc agatgaaact caaactctgt cgcaggtgaa
    42841 ctggcaccag ctggcacata taactcacca actttcgtcg ccgcctggcc gcatgtattg
    42901 aacattaaca gcaataaaag acataaaaca tgacgaagga ttttcatttt aaatatactc
    42961 aaaagttgct gtcaggctgg cggtgaaatc accgatggtt acatcacgac cgcttttcgc
    43021 ctggatccag gcccggaaat taatatcatt gttgccgttg gtaagcgcaa aggtcgcccc
    43081 ggtggtgttg ttaatatcca cccgcttgcc gtcgagcgtt tccatcccta tccccacgcc
    43141 ctgcgcggtg gaggtgtcat cgactgccag aaaacccggc tgaccgctgt cttcggtgcc
    43201 gctcagcgtg actttgacgt tgtatcccgc cggccctttg cagtctttca gcttaaacac
    43261 aaccggcaga tgcgcggact gcccagatac catcaaatcc cggttgctga cggtgggaaa
    43321 atggatttct gccaggttgg caccatccac caccagcgtg caggatttac tcagcaggtt
    43381 gccgtaaaaa tgcaggttgt tgtcgactgc ctgagctcct gtgctggcgg tcagcagcag
    43441 gcctgctaac agcgcataac cgggtgaata aatgtgtgct gttttcattg gtaatccacc
    43501 cgtaaggttg cgtaagcaga aaaggtcgtg tccgtcagca ccgcatcagc cgctttgacc
    43561 ggtaccgcct ccagctttgg cttctggcta aaatcggtgg cgttaatgga aagcggctcg
    43621 ttgagcttaa aacgctgacc gtcatgctga agctcaattc cgaagcccgg tacatccgtc
    43681 tcaatagcag agtcgttgta ggaggtggca gtccccgtcc aggagagtgt catattccac
    43741 tcgggatcgc ggacatccga gtcgcagctc agttcgtagt ccactttctg acgaccataa
    43801 ttgccatcaa tgctgtcgat aatgagatcg cgaaattgca cttcgatggt ttgcccgccg
    43861 ctgatgctac aggcgggcgg cgacatcagc gtaccgtgga atgtgatgtc atcttccgcc
    43921 gccagcacag ccccgcaacc agcgcacagt aaaaggagca ggaatttttt cattggtaat
    43981 ccaccaccat ggtcatcgtg gcgttaaact ccgacggcgt cagttgcacc ccgctctgtt
    44041 ttaccggcac cgctttcagc tgaggctgag tgttgacatt aaaaggcgtc cagttatttt
    44101 caccaactgc aaacagggag ttgtcggcgg cgttttctat ccgcaccccc aggccggcga
    44161 tatccgtgct gagaaccgtt tctccgttga tcacggcggt attgcctttg agctgcatcc
    44221 gtaagtcatc gctgacttta ttaccgcagt tgagggtgta tttcgcatcc tgttggtaat
    44281 tggtaccatc gatcctgttg attatcacat tgccaaattt cacagcgctt cccttcacgg
    44341 agcaaggcgg cggtccatcg accaggattc gcagcgtcag atccacctgt ttgatatcac
    44401 tgccagcaac agccaaaccg ctgatgcccc atccggccag caccagcaac cctgcgcccg
    44461 ctacaaactt gtgactcata agtccctccc ggcatcagcc tttgcggctc tgctcttcat
    44521 ccacctggca gctgttgccg gtgcatatga agtacagcgg catccgcgcg ccgtagtcgt
    44581 tgatgtaagt cagcaccggc acgctgtcca tcttcgcatt cagcggtgcg gaggttttcg
    44641 gctccatcat cagcggtgtg aatcccgccg gcgggttgcc gcctttttta gcgctggcgt
    44701 tgctgatgat cacgtagtac ggcgtcgggt tattgacggt gaagtcctgg ccgttacgga
    44761 tgagcgtcac cttatgctgc cacggacttt tcatgctggt gttctccagc gccttcggtc
    44821 gccagaacag tttgatacgc gtctgcagcg caatctgcag tgtgttcggt ttatttgatt
    44881 tcggcggaat ttcgcgcacg ttgaaataga acacgctctc gcggtcggcc ggcagtttgt
    44941 tgatatccgg cagcccctgc accttcacct ggccgttcat catcgcatcg atgcgctgca
    45001 ctggcggaag caccgcaagc ggcgagctga ttttgttgcc ctttgcatcc tctatccagc
    45061 tctgcgccag ataaggcagt gtgggatcgt tgttgcgcag cgtgacgctg atggacttgt
    45121 cgctttcgtt aaacaccagc cgtgtcctgt cgggcgtcac cgaagcgtgg ctggagaaac
    45181 tgaccgctgt cgccagcagc gccagtgccg ggacaacgat ttttgcagtg ttagtcagat
    45241 taaacatgga tcattcctta atcacgttac tggtttactg aaattgaagt cggaggttcc
    45301 gttggcgtca cccgctgtgt ctgttcctga atcgccggtt tgaccgccgg cgaagtgata
    45361 ggtgctacat tgcctttatg ctggcatggc agtaacaagc cgttaaacag atcggcaggc
    45421 agcgggtccg gcaggttgat atcgcaatgc gcaacaccac tccagaacac actcatgtgt
    45481 tcgccaggtt tcaccccagc cagataaaca ttgccgtcat catcgacaag gcccactgtc
    45541 tgctcgttat cattttttac ttctgcgcca aacggaggat ggctgccgtc ttgtaaacgc
    45601 agcaccgcca tcgctttttg accactgatg acggcaaatt tgcggtagcc aatcgcccct
    45661 tcagttagcg tggcttgtac caccgactgg gttgcttcag cgttttcagg caatctgttg
    45721 aggtcgatat acgcctgatt gcggtaatag ttattgacgt cagaaacgac ggctttacca
    45781 aacatattgg tataaacagc agcaccgtta ccttctaccg gaacatcggc aacgccatcg
    45841 gcatcaatca acaagcgtgt cccgcccata ttctgggtac ggtgaagtgc gccaccgtgg
    45901 gcagtaagcg ttgcgccgcc ctgtaacgac aagcccgcag aggtgtattg cccttcatgg
    45961 tagttcgcac tgaggtcaac ctgcgccagc gaaccatcat ggctgtaata gccgtcaacg
    46021 ctggtgtgtt tgtcactggt gccgacgttc aactgatagt gagtcgcgtc atcgacacgg
    46081 ctgaaataac cgacctgact gctgtccgtc ccactgccat agttaccgtt atagctaacg
    46141 gtactgttgt cgccccacgg catactgagc gaaatgtaca tgcctttgtc ggcctggttg
    46201 tcatactcgt aacggtagcc agtcatcgag atgctgacat tacgaatact gcccatattg
    46261 aaatagtgcg agagcatgat gttgtagttt gtctgctcct cgcgatccca gtaggtatgg
    46321 cgggtatagt tgagataaac cgaaacaccc gcatcgcgga agttctggtt ataagtggcg
    46381 gtgtacatct ctttgtcgtt gcccgtgcgt accattccgc tgtcactggc atccagatac
    46441 tcgctcatgg tcataaagtt ctcttccgag aagcgatatc cagcaaaagt aacgcggctg
    46501 ttgagctggt caaagtcttt ggaataactc acacggaagg agttaccgtc cagcgaacct
    46561 ttgccataag cggtgtcttt atccagtttg gtgtgcgagt gagtaacatc aaacgcgacc
    46621 gcgccgaatg tagacaaatc gcgaccgacg ccaagcgccg cagactgata gttttcatct
    46681 cccagtgcgc cgccatataa cgaccagccg ttagcgatcc cccaggaagc ttcagcacca
    46741 gaaaaaaatt caccctcgac atggtgtccc cactcttgcg gacggcccat catgatctta
    46801 tagcgaacct gacctgggcg agtgaggtat ggcatcgagg cggtgctgat gtcatattcc
    46861 tgcacctggc cgttctgttc ttcaatgcga atatgcaacg taccggagac agaatcacca
    46921 agatcctgaa tacgaaacgg tccagccgga acctgcgttt cgtaaatcac acgccccatc
    46981 tggctgacgg tcacttttgc tgtggtgtgt gccacgccgg aaatgtctgg cgcgtagccg
    47041 cgcagattgg gaggcaacat ttgatcgtca gtactgacac tgccaccaac atagttaaaa
    47101 ccatcaaaaa tatcggattt gaggtaatcc tcgcccagcg ccagtttggc ttttaatgat
    47161 ggtaacgccc gccaggcata gtagcgactc cactcccatt ttttttgagt ttcatcgccg
    47221 ctgaattcat cgtcatcatc attactgcga gtatgttgat agttagtctg ccagtcagca
    47281 cgcatacgcc acggccccag gttaaccccg accgtcccgt tgccgctgat ctcgttacta
    47341 tcatcaccgc cattttcttc atgccgggtt tgtgcgttga tgctgtagtc cgcgacgatc
    47401 ccggagatgc cgtcatccca acgtgaaggc ggatcccagt cgggataagt atattcgagg
    47461 taagcctgtg gcagtgaaat gactaatgcg gactggctta aatcagcctt aatttccatg
    47521 ccttccagtt gaccggattt caggcattta gcatcgtggc tccattgcag atttttcgcc
    47581 acgtcttctt ttaaaccaaa ctgcgctacc agttccggtg tcagacaagc atagcttttg
    47641 ctcgcgtcat cttcaccagc ataccagtaa atatcgtact cttccgccaa tggctgttta
    47701 tttagttgaa cctgtaaatt gtattttccg ggctcaacat atccctggct ggaaaaacgc
    47761 ttcagatcaa tttttgtgtc gccttttaat tccagaaaac gggaatcaaa ctgaatgtcg
    47821 tcttcagccc agacactgct ataactccct gacattgcca gcgcaatgca ccacggaaga
    47881 atccgtaatc gaaaaagaga atggtcaggc atacacgttt ctatccgttt ttattcttgt
    47941 tcggtcaaaa tccatttaaa tcccccatag cagtgtcgct atgggggtat gccttacagg
    48001 taagtaatct ggaaggtggt atttgcttcg aaattaccca gatctggcgc atcagctgcg
    48061 ccgaccagcc aggctttaaa gttcagtgtc tgcttcgctt tgcctttatt ggtagaagtg
    48121 tctttcacta ttttctggtc aatacccatg ccgcttttgt aagaggtacc cagagagtca
    48181 ccgatcgcca ggctaacatt gttaaatgcc gcaccggtat cggtattgaa aatggtgtaa
    48241 taattgccgc tatttgcaga agaaacggta ccagtaaagg tagtggtcat tgtttcctga
    48301 gtatcaaata cgcaatcttg cagacgaatc tggaaacttt tcggcgtggt ggtgccgtta
    48361 tttttcagtt tttcggcacc gatatcaccc atatcaactt ccagtttgtg atcgtccgga
    48421 acgatagaac acactgagtc ttcaatggtg ccgtaaaaac gtgcagtacc gttgttaccc
    48481 gcagcgattg ttgccgtaga ggtgaaaacg cccatcacca ttgccgcagc aatagctgtt
    48541 ttaacaaact tactcattat ttgtccttaa actataaata attaaaaata taaacattac
    48601 aatacattga ttcagtcaat acccaatgtt tatacccagc cattgaatac ggcatgcgat
    48661 ccaaaaagaa caggtcgtag aaaattgtat tctttggtgt ggggccg
//
LOCUS       DAZGVQ010000017        47297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000017 DAZGVQ010000000
VERSION     DAZGVQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.017"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..336
                     /locus_tag="ACHLAG_000943"
     CDS             <1..336
                     /locus_tag="ACHLAG_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP0654221.1"
                     /translation="LKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNI
                     FKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLL
                     VDPSSTAHE"
     gene            complement(320..958)
                     /gene="ytfB"
                     /locus_tag="ACHLAG_000944"
     CDS             complement(320..958)
                     /gene="ytfB"
                     /locus_tag="ACHLAG_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFP0654222.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            1177..1797
                     /gene="fklB"
                     /locus_tag="ACHLAG_000945"
     CDS             1177..1797
                     /gene="fklB"
                     /locus_tag="ACHLAG_000945"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP0654223.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            2106..3518
                     /gene="cycA"
                     /locus_tag="ACHLAG_000946"
                     /gene_synonym="dagA"
     CDS             2106..3518
                     /gene="cycA"
                     /locus_tag="ACHLAG_000946"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFP0654224.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(3563..4225)
                     /gene="ytfE"
                     /locus_tag="ACHLAG_000947"
     CDS             complement(3563..4225)
                     /gene="ytfE"
                     /locus_tag="ACHLAG_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFP0654225.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(4333..5298)
                     /gene="ytfF"
                     /locus_tag="ACHLAG_000948"
     CDS             complement(4333..5298)
                     /gene="ytfF"
                     /locus_tag="ACHLAG_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP0654226.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKYPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLITLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(5406..6266)
                     /gene="qorB"
                     /locus_tag="ACHLAG_000949"
     CDS             complement(5406..6266)
                     /gene="qorB"
                     /locus_tag="ACHLAG_000949"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP0654227.1"
                     /translation="MIAITGATGQLGHYVIESLKKTVPVSQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            6355..6735
                     /gene="ytfH"
                     /locus_tag="ACHLAG_000950"
     CDS             6355..6735
                     /gene="ytfH"
                     /locus_tag="ACHLAG_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP0654228.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(6864..8807)
                     /gene="cpdB"
                     /locus_tag="ACHLAG_000951"
     CDS             complement(6864..8807)
                     /gene="cpdB"
                     /locus_tag="ACHLAG_000951"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFP0654229.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLTAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            8997..9737
                     /gene="cysQ"
                     /locus_tag="ACHLAG_000952"
     CDS             8997..9737
                     /gene="cysQ"
                     /locus_tag="ACHLAG_000952"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFP0654230.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(9727..10284)
                     /gene="ytfJ"
                     /locus_tag="ACHLAG_000953"
     CDS             complement(9727..10284)
                     /gene="ytfJ"
                     /locus_tag="ACHLAG_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFP0654231.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            10609..10815
                     /gene="ytfK"
                     /locus_tag="ACHLAG_000954"
     CDS             10609..10815
                     /gene="ytfK"
                     /locus_tag="ACHLAG_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFP0654232.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(10877..12220)
                     /gene="paeA"
                     /locus_tag="ACHLAG_000955"
     CDS             complement(10877..12220)
                     /gene="paeA"
                     /locus_tag="ACHLAG_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP0654233.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(12543..13181)
                     /gene="msrA"
                     /locus_tag="ACHLAG_000956"
     CDS             complement(12543..13181)
                     /gene="msrA"
                     /locus_tag="ACHLAG_000956"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP0654234.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDQGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            13387..15120
                     /gene="tamA"
                     /locus_tag="ACHLAG_000957"
     CDS             13387..15120
                     /gene="tamA"
                     /locus_tag="ACHLAG_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFP0654235.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            15117..18896
                     /gene="tamB"
                     /locus_tag="ACHLAG_000958"
     CDS             15117..18896
                     /gene="tamB"
                     /locus_tag="ACHLAG_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFP0654236.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVNTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVSKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            18899..19240
                     /gene="ytfP"
                     /locus_tag="ACHLAG_000959"
     CDS             18899..19240
                     /gene="ytfP"
                     /locus_tag="ACHLAG_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFP0654237.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            19452..19703
                     /gene="chpS"
                     /locus_tag="ACHLAG_000960"
     CDS             19452..19703
                     /gene="chpS"
                     /locus_tag="ACHLAG_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFP0654238.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            19697..20047
                     /gene="chpB"
                     /locus_tag="ACHLAG_000961"
     CDS             19697..20047
                     /gene="chpB"
                     /locus_tag="ACHLAG_000961"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFP0654239.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(20127..20657)
                     /gene="ppa"
                     /locus_tag="ACHLAG_000962"
     CDS             complement(20127..20657)
                     /gene="ppa"
                     /locus_tag="ACHLAG_000962"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFP0654240.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            20967..21923
                     /gene="ytfQ"
                     /locus_tag="ACHLAG_000963"
     CDS             20967..21923
                     /gene="ytfQ"
                     /locus_tag="ACHLAG_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFP0654241.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            22063..23565
                     /gene="ytfR"
                     /locus_tag="ACHLAG_000964"
     CDS             22063..23565
                     /gene="ytfR"
                     /locus_tag="ACHLAG_000964"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFP0654242.1"
                     /translation="MTTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGFLRRKEMEKRATELMTSYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            23579..24601
                     /gene="ytfT"
                     /locus_tag="ACHLAG_000965"
     CDS             23579..24601
                     /gene="ytfT"
                     /locus_tag="ACHLAG_000965"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFP0654243.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            24588..25583
                     /gene="yjfF"
                     /locus_tag="ACHLAG_000966"
     CDS             24588..25583
                     /gene="yjfF"
                     /locus_tag="ACHLAG_000966"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFP0654244.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKILGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(25616..26614)
                     /gene="fbp"
                     /locus_tag="ACHLAG_000967"
     CDS             complement(25616..26614)
                     /gene="fbp"
                     /locus_tag="ACHLAG_000967"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFP0654245.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            26790..28163
                     /gene="mpl"
                     /locus_tag="ACHLAG_000968"
     CDS             26790..28163
                     /gene="mpl"
                     /locus_tag="ACHLAG_000968"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFP0654246.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPTHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(28319..28870)
                     /gene="yjgA"
                     /locus_tag="ACHLAG_000969"
     CDS             complement(28319..28870)
                     /gene="yjgA"
                     /locus_tag="ACHLAG_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFP0654247.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            28964..30316
                     /gene="pmbA"
                     /locus_tag="ACHLAG_000970"
     CDS             28964..30316
                     /gene="pmbA"
                     /locus_tag="ACHLAG_000970"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFP0654248.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            30499..30885
                     /gene="cybC"
                     /locus_tag="ACHLAG_000971"
     CDS             30499..30885
                     /gene="cybC"
                     /locus_tag="ACHLAG_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFP0654249.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(30930..31394)
                     /gene="nrdG"
                     /locus_tag="ACHLAG_000972"
     CDS             complement(30930..31394)
                     /gene="nrdG"
                     /locus_tag="ACHLAG_000972"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFP0654250.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QTFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(31552..33690)
                     /gene="nrdD"
                     /locus_tag="ACHLAG_000973"
     CDS             complement(31552..33690)
                     /gene="nrdD"
                     /locus_tag="ACHLAG_000973"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFP0654251.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(34084..35739)
                     /gene="treC"
                     /locus_tag="ACHLAG_000974"
     CDS             complement(34084..35739)
                     /gene="treC"
                     /locus_tag="ACHLAG_000974"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFP0654252.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPIYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(35789..37210)
                     /gene="treB"
                     /locus_tag="ACHLAG_000975"
     CDS             complement(35789..37210)
                     /gene="treB"
                     /locus_tag="ACHLAG_000975"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFP0654253.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(37329..38276)
                     /gene="treR"
                     /locus_tag="ACHLAG_000976"
     CDS             complement(37329..38276)
                     /gene="treR"
                     /locus_tag="ACHLAG_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFP0654254.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            38461..38514
                     /gene="mgtL"
                     /locus_tag="ACHLAG_000977"
     CDS             38461..38514
                     /gene="mgtL"
                     /locus_tag="ACHLAG_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFP0654255.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            38655..41351
                     /gene="mgtA"
                     /locus_tag="ACHLAG_000978"
     CDS             38655..41351
                     /gene="mgtA"
                     /locus_tag="ACHLAG_000978"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFP0654256.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(41557..41943)
                     /gene="ridA"
                     /locus_tag="ACHLAG_000979"
     CDS             complement(41557..41943)
                     /gene="ridA"
                     /locus_tag="ACHLAG_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFP0654257.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(42016..42477)
                     /gene="pyrI"
                     /locus_tag="ACHLAG_000980"
     CDS             complement(42016..42477)
                     /gene="pyrI"
                     /locus_tag="ACHLAG_000980"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFP0654258.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(42490..43425)
                     /gene="pyrB"
                     /locus_tag="ACHLAG_000981"
     CDS             complement(42490..43425)
                     /gene="pyrB"
                     /locus_tag="ACHLAG_000981"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFP0654259.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VIAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(43429..43563)
                     /gene="pyrL"
                     /locus_tag="ACHLAG_000982"
     CDS             complement(43429..43563)
                     /gene="pyrL"
                     /locus_tag="ACHLAG_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFP0654260.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(43844..44239)
                     /gene="yjgH"
                     /locus_tag="ACHLAG_000983"
     CDS             complement(43844..44239)
                     /gene="yjgH"
                     /locus_tag="ACHLAG_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP0654261.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            complement(44370..45083)
                     /gene="bdcA"
                     /locus_tag="ACHLAG_000984"
     CDS             complement(44370..45083)
                     /gene="bdcA"
                     /locus_tag="ACHLAG_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0654262.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDIHVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHGFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            45154..45747
                     /gene="bdcR"
                     /locus_tag="ACHLAG_000985"
     CDS             45154..45747
                     /gene="bdcR"
                     /locus_tag="ACHLAG_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP0654263.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILLDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            45892..46344
                     /gene="tabA"
                     /locus_tag="ACHLAG_000986"
     CDS             45892..46344
                     /gene="tabA"
                     /locus_tag="ACHLAG_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFP0654264.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTVETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            46466..>47297
                     /locus_tag="ACHLAG_000987"
     CDS             46466..>47297
                     /locus_tag="ACHLAG_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0654265.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHNKSSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFTQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTITDLLE
                     KPLNNALLAIRKEHLLLMPYVCNESISYLLGEKEILKEIDDLNAVNNYLLNNKKATDN
                     E"
BASE COUNT        11295 a        11812 c        12638 g        11552 t
ORIGIN      
        1 ttaaaaattg tgcatagtga aaatcaaagt aaaggtgtgc attatgcaaa atcacagcgt
       61 ctcttagaaa ttaaccatgc gcacttgcag ttaatggaat ccttattgga tgaagggaag
      121 aaacacaata tttttaagcc agatatcgac ccattgcagg tgaatattaa tattgctgcg
      181 cttgggggat attatttgat caaccagcat acgcttggcc tggtttatca tatcagtatg
      241 gtttcccccc aggcactgga agccagacgt aaggtcatca aagagacaat ccttagctgg
      301 cttttggttg acccttcatc taccgcgcac gaataaaact gccatccggc tggcgggtaa
      361 acaggacctg ttgattattc cccgtatcaa tggttaagcc cgtcaccacg ccgctggcgt
      421 tctggcggat tttcaccatt tgcccgtttt gcaaattact cagcggctta cccgcgcctt
      481 ctacctgcgc cattgcatag acatcggtag cgggcagtcc gtggtcgcgg aacagctgcg
      541 ccatcgtttt accgggttcg acacggtaag agcgccattg attatcaatt ccgctatctg
      601 gctggaatgg ttgcgtttgg gtagtttgcg gctgctcttc tggctggcct tcctgtatag
      661 gttctggagc gaccggagcc acctgatcag gatcattttg tggggtgact aattgcgccc
      721 gcagctgttc ttctgtcggc ggttgtgatt gcgactgaat atctaactgc gcttcacgcg
      781 tcaccaccgg cgcgttgggc gtatcatcag atggaagcag aaatccgacc accaatacaa
      841 tggcggcaat aatgatcccc cgacgatgca tcggcggcag cgggtccatg aagcgaaaat
      901 tgtccggcgc gtgccagact ttctccaggg ttggttttag ttcaaagcgc ccgggcatgg
      961 tttcctcctg ctccgcgtct tgttcctcaa tcatagccta tgaataagct aacgctatga
     1021 tgtccgtggt aaacccgcct ttattatatt catacgggat attgctattg tttctttttc
     1081 cccgggattt gtcatcattc ctgcgacaaa gttttaccca aagaagtgtg gctgatatgc
     1141 tgcccgctac ttttataccc taagaaagga aatacgatga ccaccccaac ttttgacacc
     1201 atcgaagcgc aagcaagcta cggcattggt ttgcaggtag ggcaacaact gagtgaatct
     1261 ggcctggaag ggctgctgcc agaagcactg gttgcaggta ttgccgatgc gctggaaggc
     1321 aaacatccgg ctgttccggt tgatgtggtg catcgcgcgc tgcgtgaaat ccacgagcgc
     1381 gccgatgccg ttcgtcgtca gcgtttccag gcgatggctg ctgaaggtgt gaaatacctg
     1441 gaagaaaacg ccaaaaaaga aggtgtgaat agcaccgaat ctggcctgca attccgcgtg
     1501 atcaaccagg gtgaaggcgc aattccggca cgtaccgacc gcgttcgtgt tcattacacc
     1561 ggtaaactga tcgacggcac cgtgtttgac agctccgttg ctcgtggtga acccgctgaa
     1621 tttccggtta atggcgtgat ccctggctgg attgaagcac tgactctgat gccggtaggt
     1681 tctaaatggg aactgactat cccgcaggaa ctggcatatg gcgagcgcgg cgcaggcgca
     1741 tccatccctc cgttcagcac cctggtgttt gaagtcgaac tgctggaaat cctctaagca
     1801 gcgcattctg ttcccctcga accagagggg agcaggcatt cagcaataaa cccttcagtt
     1861 tgccaaacgg cgctattttg tgttgcaaag accccgtaag cgtgtatttt tgtgagctgt
     1921 ttcgcgttat caccgtgata tgacactcac tttaaacata aaattaacat tagatctaaa
     1981 tcttagtatt catcccgcgt attgttacct aatatcgatg agtcccgata cagattcgtc
     2041 gtatcataga ctgactaaag gccgtagagc ctgaacaaca cagacaggta caggaagaaa
     2101 aaaacatggt agatcaggta aaagtcgttg ccgatgatca ggctccggct gaacagtcgc
     2161 tacggcgcaa tctcacaaac cgacatattc agcttattgc cattggcggt gccattggta
     2221 cggggttgtt tatggggtct ggcaaaacga ttagccttgc cgggccgtcg atcattttcg
     2281 tttatatgat cattggtttt atgctctttt tcgtgatgcg ggcaatgggg gaattgctgc
     2341 tttcgaatct ggaatacaaa tcttttagtg acttcgcttc cgatttactc gggccgtggg
     2401 caggatattt cactggctgg acttactggt tctgctgggt tgtaaccggt atggcagacg
     2461 tggtggcgat cacggcttat gctcagttct ggttccccga tctctccgac tgggtcgcct
     2521 cgctggcggt gatagtgctg ctgctgacgc tcaaccttgc caccgtgaaa atgttcggtg
     2581 agatggagtt ctggtttgcg atgatcaaaa tcgtcgccat cgtgtcgctg attgtcgtcg
     2641 gcctggtcat ggtggcgatg cactttcagt caccgaccgg tgtggaagcg tcattcgcgc
     2701 atttgtggaa tgacggcggc tggttcccga aaggtttaag tggcttcttt gccggattcc
     2761 agatagcggt tttcgctttc gtggggattg agctggtagg tacaacagct gcggaaacca
     2821 aagatccgga gaaatcactg ccacgcgcga ttaactccat tccgatccgt atcattatgt
     2881 tctacgtctt cgcgctgatt gtgattatgt ccgtgacgcc gtggagttcg gtagtcccgg
     2941 agaagagccc gtttgttgaa ctgtttgtgt tggtaggttt gcctgcggct gccagcgtga
     3001 tcaactttgt ggtgctgacc tctgcggcgt cttccgctaa cagcggcgtc ttctctacca
     3061 gccgtatgct gtttggtctg gcgcaggaag gtgtggcacc gaaagcgttc gctaaacttt
     3121 ctaagcgcgc agtacccgcg aaagggttga ccttctcgtg tatctgtctg ctcggcggcg
     3181 tggtgatgct gtatgtgaat cccagcgtga ttggcgcgtt cacgatgatc acgaccgttt
     3241 ccgcgattct gtttatgttc gtctggacga ttatcctttg ctcgtacctg gtgtaccgca
     3301 aacagcgtcc tcatctgcat gagaagtcga tctataagat gccgctcggc aagctgatgt
     3361 gctgggtatg tatggcgttc tttgtgttcg tggtcgtgtt gctgacactg gaagatgaca
     3421 ctcgccaggc gctgttggtc accccgctgt ggtttatcgc actggggctg ggctggttgt
     3481 ttattggtaa gaagcgggct gctgaactgc ggaaataacc gcattatcat gctggatggc
     3541 gcaatgccat ccagctttta gattactcac ccgccagcgc gcgtgggaac agtacattgt
     3601 tttccaagct gatgtgatcc atcaggtcat caatcagttc attaatgccg ttatacatcg
     3661 ctttccaggt ggtgcaggct tctggcggcg gtgtgacgtt attggtagtg tgtttgatca
     3721 cttccagcag ttcgcccgct tcatcgtgct cgctttccat tacgctgatt ggccccattg
     3781 cctggctgcc catgccttgt ttgatcatcg ggaagaggat ctgctcttct ttcatcatgt
     3841 ggctggaaag ctcttcatgc agcatagtca ggtattttgt cagccctttt ggcacgctcg
     3901 gtttgtcggc gtgaacgcgc tcgactttag tcgcttgcag gatcagctcc ggcagctgct
     3961 cgcggtgacg atcgtggtag cgcacgatga tatggtcaat gatttctgcc agcggggcgc
     4021 tacgccagtc tttctcaatc ggttgttcag caagctttgc cagttcagct tcaatgacct
     4081 caacatccag ttctttacgt gccgccgcgc gcgccagcgt ctgcttaccg ccacagcagt
     4141 aatccatatc atatttacga aacagagccg aagcgcgagg gatagagagc gccagttcac
     4201 ctaaaggttg gtcgcgataa gccatagctg atacctcatt cttaataata taagatgtat
     4261 tttaaatgca tctttaaggc aaaagctata acccttacgt agcagaggct tatttaactc
     4321 actgccagcg tgtcaacttt ctgagacaga ttcagttaaa ggtttttctg gttttgctct
     4381 gaccgcaatg accacgccaa tcactaacag cgcgataccg ctcagcgtta ttagcggcgg
     4441 catttgctgg cggagtaaaa aggtgtacag caaacctgcc agcgtttcga aaacaatcag
     4501 cggcccgaga atcactgtcg gtaatcgctg gctggcgacg ttccagcaga gtgcgccaac
     4561 ccatgagcaa agcacggcta tcgcaaccat cagactaata aacaccagcg gacgggggcc
     4621 aaaaggtaag gagaagtccg gcgtttgtat attcagccag taacaggcga cgagatagcc
     4681 gatgagagaa accggcagcg tgaccagcgc ctgcgccgtc gcccacatca tcggatattt
     4741 gtcgggattt tcccgcagcc agcgggcgtt gcgcagggca taccatgccc agcagaccac
     4801 ggaaactaac gctagcacga tgccagaggt ataacgtgcc cagtcaaaat cggggagtcc
     4861 gtggtttaac tcagcaatat tcacactcgc caggccgatg ccaatacaaa tcagtgccgg
     4921 ggcgagtttt ccccacgcga gtttgccgtc gcgctggcta taaagcagat tggcaaagac
     4981 aggaatgacc accggcaggg tgccgataat catcgtggaa acaggcgcgc cagtacgttg
     5041 aatggcactg gcaaggcaga agtaataaat gaggttgccc atcatagtga gcatcaaggc
     5101 ggtaagccag tcccgacgcg ccaactgacg cagacgcacg cgtcccagcc aggcaatggg
     5161 cagcgcaatt aaccctaacg ccagataacg ccccatcgac tgcaacatcg ccgggtattc
     5221 cggtacgatc aacggcccga caaaaataag cccccacatc aaccctgcta acagggcgta
     5281 cagcacgccg ctaatcatta ctggcatcca ttggtctgtc agaagaaagt tcaggaagca
     5341 gacatgattg ctcccggcat cccgtcatta ttagagagga tgcgggagga tgccacttta
     5401 attaactagt tattaacatt aaaaagatgg cttacgcttt cggctaacgt tgtcgttggg
     5461 cggccaatca atttgctaag cgttttgctg ttatcaaaca gaccaccttt cgatgcgcca
     5521 acgtcagaat ccgccagcat atccgccagt ccgtcgggca gtccgacgct tttcagtgcc
     5581 gcggcgaaat cggcttcgct cagattttga taggtaacct gtttgccgct ctgtttggtc
     5641 agctctgccg ctaactgtgt caatgtccag gcactatcgc ccgccagttc gtaaaccttg
     5701 ccttcgtgac cggcttcgct aatcacgcgt gccgcagctg ccgcataatc tgcccgcgtt
     5761 gctgaggcga ttttgccatc gcccgccgca ccgataaata cgccgtgttc cagtgctgcc
     5821 ggggcgctgg cgaggtagtt ttcgctgtac cagccgttgc gcagcagggt gtaaacgatg
     5881 ccagaatcag ccaacatttt ctccgtctcg atgtgctcat cggcgaggcc gagcggggag
     5941 gtatctgcat gtagcaggct ggtataagcg ataaatttca caccagccgc ctttgcggca
     6001 ttaataacat tacgatgctg cggggcacgt tgacccactt cgctggaaga gatcagcagt
     6061 agtttttcca ctccctgaag tgcagatgtc agtgcggctt catcgccgta gtcagcctga
     6121 cgcacggtaa tgccttgtgc tgccagagcc tgggcttttg ccggattacg aacgatagcc
     6181 actatttggc tgacaggaac cgttttcttc aaggattcaa taacatagtg accaagttgg
     6241 ccagtggcac cagtaatagc gatcatggga agtctccatc gttttgtctt gtgtttaaat
     6301 acgctaacac ctaaacttac ttttagtaag tacgcacaaa aaggtaagta tgaaatgagt
     6361 caggttagcc tgtcgcaaca actgaaagag ggtaatctct ttgcggaaca gtgcccgtcg
     6421 cgcgaggtgt tgaaacacgt caccagccgt tggggggtgt tgattctggt ggcgctacgc
     6481 gaaggtactc atcgctttag cgacctgcgg cgcaaaatcg gcggggtgag cgaaaagatg
     6541 cttgcgcagt cgttacaggc gttagaacag gatggttttc ttaaccgtat cgcgtatccg
     6601 gtggtgccgc cgcatgtgga atatagcctc acgccgctgg gcgagcaggt gagcgaaaag
     6661 gttgctgcac tggcggactg gattgagttg aatttgcccg aggtgttggc ggtgcgggat
     6721 gaacgtgcgg cataactgcc ggatgcgctg agcttatctg gtctacggtg catggcttgt
     6781 agggcggata agatgcgcca gcatcgcatc cgccaataaa tgccggatgc ggttgatgat
     6841 ttataggccg aaaaagaagt gttttactta ctcaaatcca cctgataaat cgcaaacccg
     6901 atatcatcgg tcgcgacttt attcatcgga tattgccctt tctctttaat aaacgctgcg
     6961 gctttatctg acggagaagt ttcgaaacgg atatccagtt tcttatcgcc agctatcggt
     7021 gctaaacgcc agttgttatc tgccgccggg tgaatttccc ccgcacgttt cgactcatca
     7081 gcaatccacg ctgccagcac cgagcggttc tcatccggtg aagcaaaagc gatatggctg
     7141 tcgcccgtac cggcaaattt gccgccgtaa gcgcgatagt tattggtggc aacgaggaac
     7201 atggcgttcg gatcaatcgg cttgccatta aaggtcaggt tcttaatcct ttccgcattg
     7261 gcattaatca tctggcactc gccgtcataa cgggcaggct gggtaacatc aatctgataa
     7321 ttcacaccat caatcacatc aaagttatag gtgcggaaac catcccagtt gatgagtgat
     7381 tgcggtttcg tgctgttagg atcaatctgg ttaaactgtc cggcggagca ctccagccac
     7441 tctttcacct ctttaccgct ggctttcact acaatcagcg tattggggta gagataaaga
     7501 tcggcggcat tacggaaggt caactggcct ttttccacct ccacatagct tgccgggtca
     7561 tttttgcgac caccaacttt aaacggtgcg gcagctgaaa gcaccggcag ttttgccaga
     7621 tccggatcgc cctgaatgta atgttcgaca tacgcttttt gcgcgttgtt cactacttgc
     7681 acggtcggat cgtcctgcac cagcgccaga tagctataca tattgtcggc agatttaccg
     7741 attggcttgc tgacgaactg gcgtgtggca tcgtgatcgg ctttgagtgt ttctaccagc
     7801 ttgctgtctt ccgccgtgag ggatttttta ttggcgatgt cgtaaatcgg ccgagcttcc
     7861 gctttcgcct gcgtcacctg ccatttaccg ctgtcattac tgagttgtaa gtcgaccacc
     7921 ccaagatgat cgccccacat gcccggcatt accgccggaa caccattcag cgtgcctttg
     7981 gtgatatcag ccccttcgat atcagcaaaa tctttacccg ggaaaacggc gtgagcatga
     8041 ccaaacataa tggcgttaac gcccggaatt tcactgaggt aataaactga gttttccgcc
     8101 atcactttat acggatcggc agacagcccg gaatgtgcca gaacgacaac gacatcggca
     8161 cctttctcgc gcatttcagg cacgtatttg cgcacggttt cggtaatatc attcaccgtc
     8221 actttcccgg ataaattagc tttatcccag cccatgattt gcggcggtac gacgccaata
     8281 tagccaatct tcagcgtctg tttttttccg tctttatcga ccacttcggt gtctttaatt
     8341 aaatacggtg taaacattgg ctgtttggtt ctggcgtcaa tgacgttggc atttacataa
     8401 gggaatttcg ctcccgccag cgcatttttc aggtaatcca gaccgtagtt aaactcatgg
     8461 ttgccaagcg tgccgacggt atagtccagc gtatttaatg ccttatacac tgggtgaata
     8521 tcacctgctt ttaatccttt cgccgacatg taatcggcca gcggactccc ctgaatcaaa
     8581 tcgccgttat cgaccagtac gctgtttttc acttcattgc gggcatcgtt aatcaggctt
     8641 gccgtacgta ccagtccgaa tttttccgtg gcggtgtctt tgtaataatc gaaatccatc
     8701 atgttgctat gcagatcagt ggtttccatg atacgcagat cgaccgtcgc tgcattcaca
     8761 ctggcggcaa tcagcgtggc caggagcgtt gcgctaaact taatcatcag ggacatcctt
     8821 ttatcatcgg gaatacgaaa gaaaagggag aataaacgtc ttacttatag aacagtgaag
     8881 aatgccacaa ttttacgctt tgaaaatgat gacactatca cagttggcgc attcattaac
     8941 gatagggtat aagtaaaaca ataagttaac accgctcaca gagacgaggt ggagaaatgt
     9001 tagatcaagt atgccagctt gcacggaatg caggcgatgc cattatgcag gtctacgacg
     9061 ggacgaaacc gatggacgtc gtcagcaaag cggacaattc tccggtaacg gcagcggata
     9121 ttgccgctca caccgttatc atggacggtt tacgtacgct gacaccggat attccggtcc
     9181 tttctgaaga agatcctccc ggttgggaag tccgtcagca ctggcagcgt tactggctgg
     9241 tagacccgct ggatggtact aaagagttta ttaaacgcaa tggcgaattc accgttaaca
     9301 ttgcgctcat tgaccatggc aaaccgattt taggtgtggt gtatgcgccg gtaatgaacg
     9361 taatgtacag cgcggcagaa ggcaaggcct ggaaagaaga gtgcggtgtg cgcaagcaga
     9421 ttcaggtccg cgatgcgcgc ccgccgctgg tggttatcag ccgttcccat gcagatgcgg
     9481 agctgaaaga gtatctgcaa cagcttggcg aacatcagac cacgtccatc ggctcttcgc
     9541 tgaaattctg cctggtggcg gaaggacagg cgcagctgta cccgcgcttc ggaccaacga
     9601 atatttggga caccgccgct ggacatgctg tggccgcagc tgccggagcg cacgttcacg
     9661 actggcaggg taaaccgctg gattacactc cgcgcgagtc gttcctgaat cctgggttca
     9721 gagtgtctat ttattaatta atttatgcag caggtccatc acttgctgca cctcttcctg
     9781 agtaagcgcc ccgtctttgg cccattgcac gcgaccgtct ttatccagca ccactacagc
     9841 ggaacttttt tcatccagct gccaggcacc gcgtgcgacg ccattgctat caacaataaa
     9901 ctgcgaccag ggataaagct ttttattact ctccagactg ctgcgcacaa acatgccgga
     9961 acccggaatt gcgtcgtcgg tgttaacaat ggtggtggtc tggtaacgat cgtgcggtaa
    10021 cttcgctgat ttaatcgctt caatcagcgt cgcgtttttc tcttttgcag aggtgcgacc
    10081 agcaatatgt tgcagtactc gcacttttcc cactaactgc gcgctgttcc aggttttgta
    10141 gctaaactga tctttatcaa gcaccaactc gccacgatcg gtgatgccaa tcggcggcac
    10201 tcgctgaccg gtttcgaact gatgtgcgga agccatcatc ggcaacagca ggcaggtgag
    10261 tgccagaatc ttgcgtaggg tcatggtgtt tccttcttat gatatgcagg tgatccgacc
    10321 acttgggtct gagttttaat catatgtgct atttatcgaa tttcccgcaa gtgtgatgcc
    10381 agtttgcggt caagcgcaca aatcatatga aaaatgaatg cttatactga agaccgcgct
    10441 tcggtaaaaa gataattctg aataattgta acctttaggt aaaaaaagtt atacgcggtg
    10501 gaaacattgc ccggatagtc tatagtcact aggcattaaa atttgcgcct cataatagtt
    10561 gggccgattg tggcaccgca caggcgtaat actcagcagg agataacaat gaaaattttc
    10621 caacggtaca acccacttca ggtggcgaag tacgtaaaga tcctgttccg tggacggtta
    10681 tacatcaagg acgttggcgc ttttgaattt gataagggta agattcttat cccaaaagtg
    10741 aaggataagc tgcatttgtc agtgatgtcc gaagttaacc gtcaggttat gcgtctgcaa
    10801 acagagatgg cttaaccaaa gtgctatgca gtaataagac ggctcctgat tcaggagccg
    10861 ttgatgtttc tgggggttac gcgacgcttt cttctttatc tttagcgtca ggcagttttg
    10921 gcgaaagggc ggtggccttg ctgtcgatcc gggtcaccag cagctggtcg atgcggtagt
    10981 tatcgatatc caccacttca aatttgtagc cggcgaattt caccgaatcg gtgcgtttcg
    11041 ggatcttacg cagcataaac atcataaagc ctccgatggt ttcgtagttg cccgactgcg
    11101 ggaactcgtc aatatccagc acgcgcatga cgtcgtcaat tggggtgccg ccgtcaatca
    11161 gccatgagtt ctcatcacgg gcgacaatct gctcttccag cccctgaccg accagatcgc
    11221 ccatcagcgt ggtcatcacg tcgttgaggg tgatgatccc caccaccagc gcgtactcgt
    11281 tcatgatcac cgcgaagtct tcacctgcgg ttttaaaact ttccaacgcc tctgaaaggg
    11341 ttaacgtatc cggcacaatc agcgtgttgc gaatttgtac gccgctgttc agtgccaggc
    11401 tttggttagc cagcacgcgg ttcagcaggt ctttagaatc gacataaccg atgatgtggt
    11461 caatatcttc attacagacg aggaacttag agtgcggatg ttccgccacc ttattcttca
    11521 ggctttgctc atcttcgtgg agatcaaacc aaatcacgtt ttcacgcggt gtcattgaag
    11581 acggaacggt acgggattcc agctcaaaga cgttttcaat cagctcgtgt tcctgtttac
    11641 gtaacacgcc cgccagcgca ccggcttcca ctaccgcgta gatgtcatca gaagtgatgt
    11701 catctttacg taccattggc agtttgaaaa tacggaagat catgttcgcc aggccgttga
    11761 agaaccacac cagcggggtg caaacgtaca ggcagaagcg catcgggttg atgatacgca
    11821 aagccaccgc ttctggcgca atcataccga tgcgtttcgg ggttaaatcc gcaaacagga
    11881 taaacatgcc agtcactaac gagaaagaga gaataaagct cagttgctca gagagttctg
    11941 ccgacatata gcgggagaac aggctgtgaa aagctggaga aaatgccgca tcaccgacga
    12001 taccgccgag aatcgcaact gcgttcagac cgatttggac caccgtaaag aacatgccgg
    12061 gattttcctg catattcaga acgcgttggg catttatatt gccttcatca gccagcagtt
    12121 taagtttgat tttgcgtgag gcggcaagtg agatctcaga catcgagaag aacgcactta
    12181 cagcgatcaa gcagagtatg actaaaatac tgtttaacat atcttatccg acctttcagg
    12241 ccagatcctc ggaaggggaa gttgattaat ttgtgtgaaa tacacattga aagccgattc
    12301 gaagaaagtg aaccggcatt ttcagtggct agtatagcgt aaggtactgt aaagccgcca
    12361 gagggttaaa attcagacag ctgaaaaatg caaaactgcc tgatgcgcta tgccgatcag
    12421 gcctacgcag tccttgcaat atattgaatt tggacgattt tgtaggcctg gcaaggcgtt
    12481 tacgccgcat ccggcataaa caaaacgcac tttatcaaca atctacattt atacccgtaa
    12541 cgctatgctt ccggcggcag acagacgcca attccgccaa ttccacagta gccatacggg
    12601 tttttatgca gatattgctg gtggtcatcc tcggcataat aaaacggtgt ggcgttagcg
    12661 atttccgtgg tgatgcgacg atcgtcatcg gcggcaagca tcgccgcctg aaaacgttcc
    12721 agactggcgc gagctgcggc atcctgttct ggggtcagcg gataaatcgc tgaacgatac
    12781 tgcgtgcctt ggtcattgcc ctgacgcatt ccctgggcag gatcgtgatt ctcccaaaat
    12841 acctgtagca actgctcata gctgatgacg gaaggatcgt aaacaatgcg taccgcttcg
    12901 gcatgaccag tatcaccgga gcacacttcc cgataagtcg gatttggcgt atagccgccg
    12961 gtataacctg cggcggtgct gtaaacgccg ggtaactgcc agaacagacg ctccacaccc
    13021 cagaaacaac ccatcgcaaa aatggcaatc tccattccgt caggtacatt ggtcattgag
    13081 tgaccgttga ccgcatgcag cgtggctacg ggcatcgggg tgttacgtcc aggcagggca
    13141 tcggcggggg aaacaagatg ctttttatca aataaactca tggtgtcgct ctcccgaaaa
    13201 tcggtcattg gggttaaggt tgtaacaaga ggcgtatttg cacacaataa ccaccgtgaa
    13261 taggtctaaa gtaaaacata agaaatattt ggggtttagt ctgcttttta atccatatta
    13321 ctggattttt gttaagccgt ttaacggcgt tccaggggca ggaaaaaagg atattcagga
    13381 gaaaatgtgc gctatatccg acagttatgc tgtgtaagct tactctgctt aagcggatct
    13441 gccgtcgccg cgaacgtccg tctacaggtc gaggggttat cgggacagct ggaaaagaac
    13501 gttcgtgcgc agctttctac gattgaaagt gatgaagtga cgccagaccg tcgctttcgc
    13561 gcacgcgtcg atgatgccat ccgcgaaggt ctgaaagcgc tgggttatta ccagccgacc
    13621 attgaatttg atctccgtcc accgccaaag aaagggcggc aggtattgat cgccaaagtt
    13681 acgccaggcg tgccggtgtt aattggcggc accgatgtgg tattgcgcgg cggcgcgcgg
    13741 accgataaag actatttgaa attgctcgat actcgcccgg ctattggcac ggtactgaac
    13801 cagggcgatt atgaaaattt caaaaagtcc ttaaccagca ttgcgttgcg taaaggttat
    13861 ttcgatagcg aatttaccaa agcgcagctg ggcattgcgc tcggcctgca taaagccttc
    13921 tgggatattg attataacag tggcgaacgt taccgctttg ggcatgtgac ctttgaagga
    13981 tcacaaatcc gcgatgaata cctgcaaaat ctggtgccgt ttaaagaggg cgatgagtac
    14041 gaatcgaaag atctggcgga actgaaccgc cgactttctg ctaccggctg gtttaactcg
    14101 gtggtggtgg ctccacaatt tgataaagcg cgcgaaacga aagtattacc cttgacgggc
    14161 gtggtttcgc cgcgaactga aaacaccatc gaaaccgggg tcggttactc tacggacgtg
    14221 ggaccgcgcg tgaaagcgac gtggaaaaag ccgtggatga actcttatgg tcacagtctg
    14281 accaccagta ccagtatttc cgcgccggaa cagaccctcg acttcagcta taaaatgccg
    14341 ctgctgaaga atccactgga acaatattat ttggtgcagg gcggttttaa gcgcactgac
    14401 ctgaacgata ccgaatctga ctccactacg ctggtggctt ctcgctactg ggatctctcc
    14461 agcggctggc agcgtgccat taacctgcgc tggagcctcg accactttac ccagggtgaa
    14521 attaccaaca ccacgatgct gttttatcct ggggtgatga ttagccgcac gcgttctcgt
    14581 ggtggcctga tgccaacctg gggcgactcg caacgctact ctatcgacta ctccaacacg
    14641 gcctggggct cagatgtcga tttctccgtt ttccaggcgc agaacgtctg gatccgcaca
    14701 ctgtacgatc gccatcgttt tgtgacacgc ggcacgctgg gctggattga aacgggtgat
    14761 ttcgacaaag taccgccgga tctgcgtttc ttcgccgggg gcgatcgcag tattcgcggc
    14821 tacaaataca aatctatcgc cccgaaatac gccaacggtg acctgaaagg ggcctcgaag
    14881 ctgataaccg gatcgctgga ataccagtac aacgtgaccg gaaaatggtg gggcgcggtg
    14941 tttgtcgata gtggcgaagc ggtaagcgat attcgccgca gcgactttaa aaccggtacc
    15001 ggggtcggcg tacgctggga atcgccggtc gggccaatca aactcgattt tgccgtaccg
    15061 gtcgcggata aagacgagca cgggttacag ttttacatcg gtctggggcc agaattatga
    15121 gtttatggaa aaaaatcagc ctcggcgtgg ttatcgttat cttactgttg ctgggatcgg
    15181 tggcgtttct ggtgggcacc accagcggcc tgcatcttgt atttaaagcg gcggatcgct
    15241 gggtgccagg actggatatt ggcaaggtca ccggcggctg gcgcgatctc accttgtctg
    15301 acgttcgtta tgagcagcca ggcgtggcgg taaaagcggg taatctgcat ctggcggtcg
    15361 gtcttgagtg cctgtggaac agtagcgttt gtattaatga cctggcgctg aaagacattc
    15421 aggtcaacat cgacagtaaa aaaatgcccc cttctgaaca ggttgaagaa gaggaagata
    15481 gcggtccgct ggatctctcc acgccgtatc ccatcaccct gacacgggtg gcgctggata
    15541 acgtcaacat caagattgat gacaccacgg tatcggtgat ggacttcacc tccggcctga
    15601 actggcagga gaaaaccctg accctgaaac cgacgtcgct gaaaggcctg ttgattgccc
    15661 tgccgaaagt ggcggaagtg gcgcaggaag aagtggtcga accgaaaatt gaaaatccgc
    15721 agccggatga aaagccgctc ggcgaaacgt tgaaagatct cttttctcgt ccggtattgc
    15781 cggaaatgac cgacgtgcat ttgccgctta acctgaacat tgaagagttt aagggcgagc
    15841 agctgcgcgt gacgggcgac acggacatca ccgtgaacac catgctgctg aaagtaagca
    15901 gcatcgacgg taatactaaa ctggacgccc tggatatcga ttccaatcaa gggatcgtca
    15961 acgccagcgg cacggcacag ctgtcagaca actggccggt ggatatcacc ctcaacagca
    16021 cactaaacgt ggagccgttg aaaggtgaaa aagtgaagct gaaagtgggc ggcgcgctgc
    16081 gcgaacagct ggagattggc gttaaccttt ccggtccggt ggatatggat ttacgcgccc
    16141 agacgcgact ggcggaagct ggattgccgc tcaacgtgga agtgaacagc aaacagcttt
    16201 actggccgtt cactggtgag aagcagtatc aggcggatga tctgaaactg aaacttaccg
    16261 gcaaaatgac cgattacacg ctctctatgc gtacggcagt gaagggacag gagatcccgc
    16321 ccgcgactat taccctcgac gcgaaaggta atgaacagca ggtcaatctc gacaaactca
    16381 ccgttgcggc gctggaaggc aaaactgaac tcaaggcgtt gctcgactgg caacaggcca
    16441 ttagttggcg cggtgagcta acgcttaatg gcattaacac cgccaaagag ttcccggagt
    16501 ggccgtcgaa actcaatggc ttgattaaaa cccgcggtag cctgtacggc ggcacctggc
    16561 agatggaggt gccagaactg aagctgaccg gtaacgtcaa acagaacaaa gtgaacgttg
    16621 acggcacgct gaaaggcaac agttatatgc agtggatgat cccagggctt catctggaac
    16681 tggggccaaa cagtgccgaa gtgaaaggcg agctgggggt aaaagatctc aatcttgatg
    16741 ccaccatcaa cgcgccgggg ctggataacg cgctgccggg gcttggcggt acagcgaaag
    16801 ggctggtgaa agtacgcggc acggtggaag cgccacaact actggcagat atcaccgcgc
    16861 gcggcctgcg ctggcaggaa ctttccgtgg cgcaggttcg cgtggaaggc gacattaaat
    16921 ccaccgatca gatcgccggg aaactcgacg tacgcgttga gcaaatttcg cagccggatg
    16981 taaatatcaa cctcgtcacc ctgaatgcca aaggcagcga aaagcagcac gagctacagt
    17041 tgcggattca gggcgagcct gtctccgggc agcttaatct ggcaggaagt tttgatcgca
    17101 aagaagaacg ctggaaggga actcttagca atacccgctt ccagacgccg gtcggcccgt
    17161 ggtcgctgac ccgcgatatt gcgctggatt accgcaataa ggagcaaaaa atcagcatcg
    17221 ggccacactg ttggcttaac ccgaatgcgg agctgtgcgt gccgcaaact atcgatgcgg
    17281 gggccgaagg gcgtgcggtg gtgaatctca accgcttcga cctcgccatg ctgaaaccgt
    17341 ttatgccaga aaccactcag gccagcggta tcttcacggg taaagcggat gttgcctggg
    17401 acaccacgaa agaggggctg ccgcagggca gtatcaccct ttcggggcgt aacgtgcagg
    17461 taacgcaaac cgtcaacgat gcggcgctgc ccgtggcgtt tcagacgctg aatctgacgg
    17521 cggaattgcg taacaaccgt gccgaattgg gctggaccat ccgcctgacc aataacggcc
    17581 agtttgatgg acaggtgcag gtgaccgatc cgcaaggccg ccgtaatctt ggtggcaacg
    17641 tcaatatccg taacttcaac cttgcgatga taaaccccat ctttacccgt ggggaaaaag
    17701 cagcggggat ggtgagtgcc aacttgcgtc tgggtggtga tgtgcaaagc ccgcagttgt
    17761 ttggtcagct tcaggttacg ggtgtggata tcgacggcaa ctttatgccg tttgatatgc
    17821 aaccgagcca gcttgcggtc aactttaacg gtatgcgctc gacgcttgcc ggtacagtac
    17881 ggacccagca gggtgaaatc tacctgaacg gtgatgccga ctggagccaa attgaaaact
    17941 ggcgggcgcg ggtaacggcg aaaggcagta aagtgcggat caccgtgccg ccgatggtac
    18001 gaatggatgt atcgccagat gttgtattcg aggctacacc aaacctgttt accctcgatg
    18061 gtcgcgtgga tgtcccttgg gcgcgcatcg tggtgcacga tctgccggaa agcgcagtag
    18121 gcgtctccag cgatgtggtg atgcttaacg ataacctgca accggaagag ccgaaaacgg
    18181 cgtcgattcc gattaacagt aacctgattg tccacgttgg caataatgtg cgcattgacg
    18241 cctttggcct gaaagcgcgg ctgacgggcg atcttaacgt cgttcaggac aaacaagggc
    18301 tgggcctgaa cgggcagatc aacatccctg aagggcgctt ccatgcctat ggtcaggatc
    18361 tgattgtgcg taagggtgag ttactgttct ctggtccgcc ggatcaaccg tatcttaata
    18421 ttgaagctat tcgtaacccg gatgctacag aagacgacgt aatcgccgga gttcgcgtca
    18481 ctggtctggc ggacgaaccg aaagcggaga tcttctctga cccggcgatg tcgcaacaag
    18541 ctgcattgtc ttatttgcta cgtggacaag ggctggagag cgatcagagc gacagtgcgg
    18601 caatgacctc gatgctgatt ggtctggggg ttgcgcaaag tggccagatt gtgagtaaaa
    18661 tcggcgagac gtttggcgta agcaatttag cgctcgacac ccagggagta ggcgactcct
    18721 cccaggtagt ggtcagcggc tatgtattgc caggtctgca agtgaaatat ggcgtgggta
    18781 tatttgactc tatagcaaca ctcacgttac gttatcgcct gatgcctaag ctatatctgg
    18841 aagccgtgtc tggtgtagac caggcactgg atttgctcta tcagttcgag ttttagcaat
    18901 gcgaatattt gtctacggca gtttacgcca caaacaaggc aacagtcact ggatgaccaa
    18961 tgcccagtta ctgggcgatt tcagtatcga taactaccag ttgtatagcc tgggccacta
    19021 tccaggcgca gttccgggga acggaacagt acacggtgaa gtttatcgta ttgacaacgc
    19081 cacgctggcc gaacttgatg ccttgcgcac caggggcggt gaatacgcgc gccagttgat
    19141 tcagacgccg tacgggagtg catggatgta cgtttatcaa cgacccgtcg atggattaaa
    19201 gctaattgaa agcggcgact ggttagacag ggataagtaa ccatatgcat acgccacctt
    19261 cgggtggcgt tgttttttgc gagacgactc gcattctgtt ttgtaattcc ctcacccttt
    19321 gcttttctct ccgagccgct ttccatatct gttaacgcat aaaaaactct gctggcgttc
    19381 acaaatgcgc aggggtaaaa cgtttcctgt agcaccgtga gttatacttt gtataactta
    19441 aggaggtgca gatgcgtatt accataaaaa gatgggggaa cagtgcaggt atggtcattc
    19501 ccaatatcgt aatgaaagaa cttaacttac agccggggca gagcgtggaa gcgcaggtga
    19561 gcaacaacca actgattctg acacccatct ccaggcgcta ctcgcttgat gaactgctgg
    19621 cacagtgtga catgaacgcc gcggaactta gcgagcagga tgtctggggt aaatccaccc
    19681 ctgcgggtga cgaaatatgg taaagaaaag tgaatttgaa cggggagaca ttgtgctggt
    19741 tggctttgat ccagcaagcg gccatgaaca gcaaggtgct ggtcgacctg cgcttgtgct
    19801 ctccgttcaa gcctttaatc aactgggaat gacgctggtg gcccccatta cgcagggcgg
    19861 aaattttgcc cgttatgccg gatttagcgt tcctttacat tgcgaagaag gcgatgtgca
    19921 cggcgtggtg ctggtgaatc aggtgcggat gatggatcta cgcgcccggc tggcaaagcg
    19981 tattggtctg gctgcggatg aggtggtgga agaggcgtta ttacgcttgc aggcggtggt
    20041 ggaataaggt gtgtttattt atcgctggca taaaaaaacc cttactaacc gaagctcggc
    20101 gttcagggtt attgcgctag aagaacttat ttattctttg cgcgctcgaa ggaagcaacg
    20161 atttcagctt tagcggcttc tgcgttttcc caaccttcaa ctttcaccca cttgcctttt
    20221 tcgaggtctt tgtagtgctc gaagaagtga gcgatctgcg ctttcagcag ctctggcaga
    20281 tcgttaacgt ctttaatgtg atcgtattct ttgctcagct tgctgtgcgg aaccgcaacc
    20341 agtttcgcat cttcaccggc ttcgtcggtc attttcagaa cgccaaccgg acggcaacgg
    20401 atcacagaac ccggctgcag cgggtacgga gtcgggacca gtacgtcaac cgggtcaccg
    20461 tccagagaca gggtgtggtt gatgtaaccg tagttgcacg gatagaacat cgcggtggac
    20521 atgaagcggt caacgaacag tgcgccgctc tctttgtcga tttcgtattt gatcggatct
    20581 gcgttagccg ggatctcaat aacaacgtag atgtcttccg gcagatcttt acccgcaggg
    20641 acgttgagta agctcatgtc tgtttccttt aaaaatatgt ggcaaacaag tgccgagtat
    20701 tatagccaac tcgcgccgaa tgtctttgct tgttttcgtc ttcattttcc tttttcgcca
    20761 gttttaagac ggtatacaga gcaggaaaat ccataaccgt agccgcattt ttcatagtga
    20821 gatgaaagcg attacaaact tgtgattaac gttttattta cttttttgaa gtgtgatgta
    20881 acgcaatccg ttacataacg aattgtctat agttttttcg cgaacatctt ttcaccaata
    20941 ataattaccc cgacgaggac aaccctatgt ggaaacgctt acttgtagtc tctgcagtct
    21001 cggcagccat gtcgtctatg gcgttggccg ctccattaac cgtaggattt tcgcaggtcg
    21061 gatcggaatc cggctggcgc gctgcagaaa ccaatgtagc gaaaagtgag gccgaaaaac
    21121 gcggaattac gttgaaaatt gctgatggtc agcaaaagca ggaaaaccag attaaagcgg
    21181 tacgttcctt cgttgcacaa ggggtggatg cgatctttat cgctccggtg gtcgcgacag
    21241 gttgggaacc ggtattaaaa gaggcgaaag atgccgaaat cccggtcttc ttgctcgacc
    21301 gttccatcga tgtgaaagac aaatctctct atatgaccac cgttactgcc gacaacatcc
    21361 tcgaaggtaa gttgatcggt gactggctgg taaaagaagt gaatggcaaa ccatgcaacg
    21421 tggtggagct gcagggcacc gttggggcca gcgtcgccat tgaccgtaag aaaggctttg
    21481 ccgaagccat taagaatgcg ccaaatatca aaatcatccg ctcgcagtca ggtgacttca
    21541 cccgcagtaa aggcaaagaa gttatggaga gctttatcaa agcggaaaac aacggcaaaa
    21601 acatctgcat ggtttacgcc cataacgatg acatggtgat tggcgcaatt caggcaatta
    21661 aagaagcggg cctgaaaccg ggcaaagata tcctcacggg ttccattgac ggtgtacccg
    21721 atatctacaa agcgatgatt gatggcgaag cgaacgccag tgttgaactg acgccgaata
    21781 tggcaggtcc cgccttcgac gcgctggaga aatacaaaaa agacggcacc atgcctgaaa
    21841 agctgacgct gaccaaatcc accctttatc tgcctgatac cgcaaaagaa gagttagaga
    21901 agaagaaaaa tatggggtat tgagggttgc tatgcctgat gccgattcgt aggccggata
    21961 aggcgctcgc gccgcatccg gcggtggtgc gctgaagcct gatgcgacgc ttaccgcgtc
    22021 ttatcatgcc tactgggagc acgctttaca ccgggggaaa ccatgacgac taaccaacac
    22081 caggagatcc tccgcaccga aggattaagt aaatttttcc ccggcgtcaa agcgttagac
    22141 aacgttgatt tcagcctgcg ccgtggcgaa atcatggcgc tgctcggtga aaacggggcg
    22201 ggaaaatcga cgctaatcaa agcattaacc ggtgtatacc acgccgatcg cggcaccatc
    22261 tggctggaag gccaggctat ctcaccgaaa aataccgccc atgcacaaca actcggcatc
    22321 ggaaccgttt atcaggaagt caacctgcta cccaatatgt cggtcgctga taatctattc
    22381 ataggtcgcg aacccaaacg tttcggcttt ctacgccgca aagagatgga aaagcgtgcc
    22441 accgaactga tgacatctta cggtttctcc ctcgacgtgc gtgaaccgct taaccgtttt
    22501 tcggtggcga tgcagcaaat cgtcgccatt tgccgggcta tcgatctctc cgccaaagtg
    22561 ctgatcctcg atgaacccac cgccagcctc gacacccagg aagtagagtt actgtttggc
    22621 ctgatgcgtc agttgcgcga tcgcggcgtc agcctgatct ttgtcactca ctttctcgat
    22681 caggtctatc aggtcagcga tcggatcacc gtcttacgca acggcagttt cgtaggctgt
    22741 cgggaaacgc gcgagctacc gcagatcgaa ctggtaaaaa tgatgctggg gcgcgagctg
    22801 gatacccacg cgctacagcg tgccgggcga acattgttga gcgacaaacc cgttgccgcg
    22861 ttcaaaaatt acggcaaaaa aggaacgatc gcaccgtttg atctcgaagt acgccccggc
    22921 gagatcgtcg gcctggcagg cttgctggga tccggacgta ccgaaaccgc cgaagtgatc
    22981 ttcggtatca aacctgctga cagcggcacg gcgttgatca aaggcaaaca gcaaaacctg
    23041 cgatcgccac atcaggcttc ggtactgggc atcggcttct gcccggaaga caggaaaacc
    23101 gatggcatca tcgccgccgc ctcggtacgg gaaaatatca tcctcgctct ccaggcccag
    23161 cgcggctggc tacgacccat ttcccgcaaa gagcagcaag agattgccga acgctttatc
    23221 cgccagcttg gcattcgcac accttcaact gaacaaccga ttgaatttct ctctggcggc
    23281 aatcagcaaa aagtgttgct ttcacgttgg ctactgaccc gaccgcaatt tctgatcctc
    23341 gacgagccaa cccgcggcat tgatgttggt gcccacgccg agatcatccg cctgattgaa
    23401 acgctgtgtg ctgacggtct ggcgctgttg gtgatctcct ccgaactgga agagctggtg
    23461 ggctatgccg atcgggtgat tatcatgcgt gatcgcaaac aggtggcgga gatcccgctg
    23521 gcagagcttt ccgttccggc gatcatgaac gccattgcgg cgtaaggaga acagtgtgat
    23581 gcctcaatct ctcccggaca ctacgccgcc gaaaaggcgc tttcgctggc caacgggaat
    23641 gccgcagctg gcggcgctat tgctggtgct gctggtcgat agcctggtag ctccgcattt
    23701 ctggcaggtg gtgcttcagg atgggcgttt gttcggtagc cccatagaca ttcttaaccg
    23761 tgcagcaccc gttgcgttac tggcgattgg tatgacgctg gtgatcgcca ccggtgggat
    23821 tgatctctcc gtaggggcgg tgatggctat cgccggagcc acaacggctg cgatgacggt
    23881 cgcgggagtc agcctgccga ttgttttgtt aagcgccctg ggcacaggca tcctggcggg
    23941 attgtggaac ggcatactgg tagcgatcct caaaattcag ccatttgtcg ccaccctgat
    24001 cctgatggtc gccgggcgcg gcgtggcgca actgatcacc tccggacaga tcgtcacgtt
    24061 taactcgccg gatctctcat ggtttggcag tggatcgctg ttgttcctgc caacgccagt
    24121 cattattgcg gtactgacgc ttctcctgtt ctggctgttg acccgcaaaa cggcactggg
    24181 gatgtttatc gaagccgttg gtatcaacat tcgggcggca aaaaatgccg gggtaaacac
    24241 gcgaatcatc gtcatgctca cctacgtgtt gagcgggcta tgtgcggcga ttgcgggcat
    24301 tatcgtggcg gcggatattc gcggtgccga tgccaacaac gccggattat ggctggagct
    24361 ggacgccatt ctcgcggtgg tgattggcgg cggatcgctg atgggcgggc gctttaacct
    24421 gctgctctcg gtagtggggg cgctgattat tcaggggatg aacaccggaa ttttgctttc
    24481 gggctttcca ccggagatga accaggtggt gaaagcggtg gtggtgcttt gcgtgctgat
    24541 tgtccagtcg caacgcttta tcagtctgat taaaggagta cgtagccgtg ataaaacgta
    24601 atttgccact gatgatcacc atcggcgtct ttgtgctggg ctatttctac tgcctgacgc
    24661 agtttcccgg ttttgcttcc acaagagtga tctgcaatat cctgaccgat aacgcttttc
    24721 tggggattat tgccgttggt atgacctttg tgatcctctc tggcggtatc gatctctccg
    24781 tcggttcggt gatcgctttt accggcgtgt ttctggcaaa agtgattggc gacttcggcc
    24841 tctcgccgtt gctggcattt ccgctggtgc tggtaatggg ctgtgccttt ggcgcattta
    24901 tggggcttct gatcgacgcc ctgaagatcc cggcatttat cattacgctt gcggggatgt
    24961 tctttttgcg cggcgtcagc tatctcgttt cggaagagtc gattccgata aaccatccca
    25021 tttatgacac gctctcaagc cttgcgtgga aaatccttgg cggcggtcgc ttaagtgcga
    25081 tggggctgct gatgctggcg gtggtggtta tcggcatatt cctcgcgcat cgtacccgtt
    25141 ttggtaatca ggtatacgcc attggcggca acgcaacgtc ggcaaacctg atggggattt
    25201 ctacccgcag caccactatt cgcatttata tgctctccac cggactggca acgctggcgg
    25261 ggattgtctt ctcgatttat acccaggccg gatatgcgct ggcgggcgta ggtgtggaac
    25321 tggacgctat cgcctcagtg gtgattggcg gtacgctttt gagcggtggc gttggaacgg
    25381 tattagggac gctttttggc gtggcgattc agggactgat tcagacttac ataaactttg
    25441 atggcacgct gagttcctgg tggacgaaaa tcgccatcgg tattttgttg tttattttta
    25501 tagcattaca gcgtggatta acggtgctgt gggagaatcg tcagagttcg ccagtgacaa
    25561 gagtcaacat tgcgcagcaa taaaaacgcc tctccgtgtg gagaggcgca ggagattacg
    25621 cgtccgggaa ctcacggata aagcgttcga catcttcaac catatggtcg ttgccgacaa
    25681 agaacgaacg gcgctggtgc agggtttccg ggataatatc cagaatacgc tctttgccat
    25741 cgctcgcttt accgcccgct tgttccgcca ggaatgccat cgggttgcac tcatacagta
    25801 aacgcagttt accgtccggg tggctggcgg tgcttgggta gagataaata ccgcctttca
    25861 gcaggttacg gtggaaatcc gcaaccagtg aaccgatata acgtgaggta tacgggcggt
    25921 tggtggattt atcttcttcc tggcagaatt taatgtactt cttcaccccg ttcggaaact
    25981 taatgtagtt tccttcgttg atggagtagg ttttgccttt ctccgggaag cgcatccgtt
    26041 cctggcacag gcagaaaacg cccagcgaag gatcgtaagt aaaggcgtga acaccgcatc
    26101 cggtggtgta aaccagcata gtagaggagc cgtataccac gtaacctgcc gcaacctgtt
    26161 tgttaccagg ctggaggaaa tcttcttccg ttaccggcgt gccaacaggc gtaacgcggc
    26221 ggtagatgga gaaaatggta ccgacagaga cgttaacatc gatgttggac gagccatcca
    26281 gggggtccat cagcaccacg tattttgcgt gttcacagcc ttcaaagacg acaatctcat
    26341 cttcttcttc agaggcaatg cccgcaacga tatcgcgtgc tttcagtgcg gctttcagtt
    26401 tttcattagc gaacaagtcg agtttctgct gaacctcgcc ctgcacgttc tcagcaccgc
    26461 tggcacccag gatatcaacc agtcctgctt tgttgatatc gcgatggata atcttggcgc
    26521 ccagttttat tgccgacagc aaagcagtga gctcaccggt agcatgagaa aactcgtgct
    26581 gcttttcgac aataaattca cctaacgttt tcataaaact ttccctgcaa tgtttatgga
    26641 gtaaagcgac cgcaacaatc ttaacaaata atctcaatgt tgcgctcagg tgaatcgcgc
    26701 cagcaaattg cggattatcc tgaaatgcgt ttctcacttg cccgacatat gcgtaaaatg
    26761 agcggcagat taaaaaagga tagtgacgta tgcgcattca tattttagga atttgtggca
    26821 cgtttatggg cggtctggcg atgctggcgc gccagttagg ccatgaagta acgggttcgg
    26881 acgccaatgt gtatccgccg atgagcacct tacttgagaa gcaaggcatt gaactgattc
    26941 agggttacga tgccagccag ctcgatccgc agccggatct ggtgattatt ggcaacgcca
    27001 tgacccgtgg aaatccgtgt gtggaagcgg tactggaaaa aaacatccct tatatgtcag
    27061 gtccacagtg gctgcacgat tttgtgctgc gcgaccgctg ggtgctggcc gttgccggta
    27121 cacacggcaa aaccaccacc gcgggaatgg cgacctggat tctggaacag tgtggttaca
    27181 aaccgggctt tgtaatcggc ggtgtgccgg ggaactttga ggtttcggcg cgtctgggcg
    27241 aaagcgactt ctttgttatc gaagcggatg agtatgactg cgccttcttc gacaaacgct
    27301 ctaaatttgt tcattactgc ccgcgtacgc tgatcctcaa caaccttgag ttcgatcacg
    27361 ccgatatctt tgacgacctg aaagcgatcc agaaacagtt ccaccatctg gtgcgtatcg
    27421 taccggggca gggccgtatt atctggccgg aaaatgacat caacctgaaa cagaccatgg
    27481 cgatgggctg ctggagcgag caggagctgg tgggtgagca gggtcactgg caggcgaaaa
    27541 agctgaccac cgatgcttcc gaatgggaag tcttgctgga tggcgaaaaa gtgggcgaag
    27601 tgaaatggtc gctggtaggc gaacataata tgcacaatgg cctgatggcg attgcggcgg
    27661 ctcgccatgt tggtgtagcg ccggcagatg ccgctaatgc gctgggttcg tttattaacg
    27721 ctcgtcgccg tctggaactg cgtggtgaag cgaatggcgt cacggtatat gatgatttcg
    27781 cccatcaccc aacggcgatt ctggcaacgc tggcggcgct gcgtggcaaa gttggcggta
    27841 cggcgcgcat tattgctgtg ctggaaccgc gctcgaatac catgaaaatg gggatctgca
    27901 aagacgatct ggcaccttca ttaggccgtg ccgatgaagt cttcctgctg caaccgacgc
    27961 atattccgtg gcaggtggca gaagtggcag aagcctgcgt tcagcctgca cactggagtg
    28021 gcgatgtgga tacgctggca gatatggtgg tgaaaaccgc tcagcctggc gaccatattc
    28081 tggtgatgag caacggcggt tttggtggca tccatcagaa actgctggat ggtctggcga
    28141 agaaagcgga agccgcgcag taattcggcc ttagcctgat atagtgtgcg taggccggat
    28201 aaggcgttca cgccgcatcc ggcatttgag catagtgcct gatgcgacgc ttgatgcgtc
    28261 ttatcaggcc tacaggtgca ccgcatccgg caaaccatac tcacatcaac aacgaaaatt
    28321 acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg gatttcggcg
    28381 gcttattccc ttctttctct ttcttcgcgt tgcggatcag agtacgcagc tgctgacgat
    28441 cggcatcggg ccacagattt aatacctcag cgatggcgtc atcaccttga tcgatcagac
    28501 gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga cggtttttca
    28561 gcttatccag cgcctgacgg atcggttcta catcgcgctg gcgcagcatc ttaccgatga
    28621 gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca atagccgcgc
    28681 gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca acaatttccg
    28741 cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg acccagataa
    28801 tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag tcttcgggct
    28861 gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa gatgcgcact
    28921 gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac ttgcaatgaa
    28981 agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt ccactgcgct
    29041 ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga ccaccggcat
    29101 tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagcg atggcgcgct
    29161 ggggatcact gtttatcacc agaaccgcaa aggtagcgca tcatccaccg atttaagccc
    29221 gcaggccatt gcccgtactg tacaggcggc gctggatatt gcccgttata cctcgccaga
    29281 tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccag atctcgactt
    29341 gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc gcgcagaaca
    29401 ggcggcattg caggcggaca aacgcattac caataccgaa ggtggcagct ttaacagcca
    29461 ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact gctcaacgcg
    29521 tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc gtgattacgc
    29581 ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg gggccgactg
    29641 tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga aagcgccagt
    29701 catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg cgatagcggg
    29761 tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac aaattctgcc
    29821 ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt cgacgccatt
    29881 cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca tcctgactca
    29941 gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg gacatgcggg
    30001 cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa tgctcaaaga
    30061 gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg ccattaccgg
    30121 tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc agtatcccgt
    30181 tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg tcaccgtcgg
    30241 taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc cggagatgaa
    30301 aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa aaattacaca
    30361 aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg tactcgcagt
    30421 acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat gaagtgtaaa
    30481 taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt ctcctcgttg
    30541 gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac cctcaacgac
    30601 aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc gttaacgaag
    30661 atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga agataaatca
    30721 ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt cggtcagatt
    30781 gacgacgcgc tgaagctggc aaatgaaggt aaagtaaaag aagcgcaggc tgctgcagag
    30841 caactgaaaa cgacccgcaa cgcctatcac cagaagtatc gttaattcct catttccctg
    30901 ttgcctgcac tcaggtaaca gggaacttat catcgcaaat gatgcaccac ctgattgctg
    30961 ctgccgcgcc agatcaggga agggtctttt aaatcctgca caaatttgcc gtcgaccagc
    31021 acgttaatca aatcaacaac ctgcatttgc gcagcgttga gttcgtcgag tttatagcct
    31081 gtccacaccc agatgtcttt acccggacac tcggcgcgga tgcgttttac cagtttcaga
    31141 atatccggca cgttttgcgg atgcagcgga tcgccgccgg agagggaaat accctggcgt
    31201 ttgatacgag tgtcattcag atcgttaata atctggtctt ccattgcttt agtaaatgtc
    31261 tgaccggaat ttacccgcca tgtgcttttg ttatagcaac cggggcattc atgaacacac
    31321 ccggagacaa acagggtgca gcgagtgccg gggccgttga cgatgtcgac aggatagtac
    31381 tgatgataat tcattgtttc gcctggctta tcagtccgcg tataggatgc atccggtaat
    31441 caatgcctga tgcgacgctg tcgcgtctta tcaggcctac agccgttgcc aaatgtaggc
    31501 cggataaggc gtttacgccg catccggcat ttgcttagcg cagagtgaag attaacctat
    31561 ctgcccattc cccaaatgtt taacgcggcg cttaacttct tcctgcttac cagcgttaaa
    31621 tggacgtgca tccgggctac ctaaatatcc gcacacgcgg cgagtcaccg acacacggga
    31681 ggcgtcatgg ttaccacatt tcgggcaagt gaagcctttg ctggtgcact cgaactcacc
    31741 ggtaaagcca cactcgtagc actcatcaat cggtgtattg gtgccgtaat acggtacatg
    31801 ctgatagctg taatcccaga catcttccag cgccttcagg ttgtgctgaa tgtttggata
    31861 ctcgccgtag caaatgaaac caccgttcgc cagcggcggg taaggcgctt caaagtcgat
    31921 cttgtcgtac gggttcacct tcttctccac atcgaggtgg aaactgttgg tgtagtaacc
    31981 tttatcggtc acgcccggca ccacgccaaa ctcagcagta tcgagacggc agaagcggtc
    32041 gcacaggttt tcactcggcg tgctgtagag actgaaacca taacccgttt cttctttcca
    32101 ctgatccact gcctggcgca gacgttcaac aatggcgata cctttcgcgc gaagctgctc
    32161 gttgtcgtaa acatgctcgc cgccgaacag cgcgttaatg gtttcgtgga tgccgatgta
    32221 acccagcgaa atagacgcac gaccgttttt gaagatttca gaaacatcat cgtcagcatt
    32281 cagacgtacg ccacaagcac cttccatata gaggatcggg gcgacgcgcg ctttcacgcc
    32341 ttcgagacga gcgatacggg tcatcagtgc cttacgtgcc agcaccagac gttcatccag
    32401 cagcttccag aaggtggctt catcgccttt tgcttccaga gcaatacgcg gcaggttcag
    32461 gctgatcacg ccgaggttgt tacgaccatc gtggatctgc tcgccgtttt cattttccca
    32521 cacgccgagg aagctgcggc agcccatcgg ggttttaaac gaaccggtga ctttcactac
    32581 ctgatcgtag ttcaggatat ccggatacat gcgcttgctt gcgcactcca gcgccagctg
    32641 tttgatgtcg tagttcggat cgcctttttt atggttcagg ccatcgcgaa tcgcaaacac
    32701 cagtttcggg aacaccgcag ttttacggtt tttaccgagg cctgcgatac ggttacgcag
    32761 gatcgattcc tgaatcaggc gcgattccca gctggtgccc agaccaaaac caaaggttac
    32821 aaacggcgtc tgaccgttgg cggtgtgcag ggtgtttact tcgtactcca gcgactggaa
    32881 ggcatcgtag cactctttga tggtacgaga gttagcgtag ccttcggcgt ccgggatgtt
    32941 ccactcttct gcggtcttgc gatgtttgtt gtagctggcg gtaacaaacg gcgccagcac
    33001 ttcatcgata cggttaatgg tggtgccgcc ataaatatgg ctggcgacct gagcaataat
    33061 ctgcgcagtt accgcggttg ccgtagagat cgacttcggc ggttcaatct cggcgttccc
    33121 cattttaaag ccctgggtca gcatgccttt caggtcgatc aacatgcagt tgaacatcgg
    33181 gaagaacggc gagtaatcga gatcgtgata gtgaatatcg ccacgctcat gtgcctgcac
    33241 cacgtcacgc ggcagcaggt gctggcgtgc atagtgttta gccacgatcc cggccagcag
    33301 gtcgcgctgg gttggaatca ccttgctgtc tttgttggcg ttttcgttga gtaacgaggc
    33361 gttggtctgc tcgaccagac cacggatctc ctggttcagg cgaccgcgtt tttcacgttc
    33421 aatgtcgcga tcgtgacggt actcgatgta agcacgagcc agttgtttgt atggacccga
    33481 catcagctgg ttttcaactg cggtctggat ctcgttgata tccacctggt tgcggccctg
    33541 catctgctcg ctgacaaccg cggcaacagt ggcgcaataa tcggcatcat cgacttccgc
    33601 tgctttagct gcacgcagaa tcgcttcttt gatgcgctct gatttaaacg gcactttgca
    33661 gccgtctcgt ttcatcacat gcggtgtcat gatcgctcca tatttttaag aacaggttat
    33721 ccacagaaat tgggaaaggc gttttccggt tttttcgttt cgttttccgg tgctttccgc
    33781 aatcgccatc cgcttatcca caacgccgca cctttttcgt ggggagctgt agtagcaatt
    33841 atagtcgatt aatacaacat attgggttgg gacgcatttt aaagtctata tatagtgctt
    33901 tgcatcaagg atgtttgaac tttttttgat gtagctcaaa gtaaaaagca gagcgtacgg
    33961 atgacgggcg ctacagcgtt atgtaaattt tttaatgaat ttgctggttg aaaaatcaac
    34021 aaaaacaaca tgctgacaga caaaaccccg gaatgaccgg cattaccggg gcttagggaa
    34081 gatttacttc tgtaaccacc agacagcctc aaaaggccgt aaattcatgg cacagggttg
    34141 tggtgaggct tcttcgtagt tatgcatcac aagctgccag ttgccgcgca tttgccctgg
    34201 ctgccagggt tggatctcac ggctaaggtt ggcaatgacc agcaaggttt gccccttcca
    34261 ttcacggcga tagcaccaca atacagggct gtttggcagc agatcctggt aattgcccca
    34321 tgtcaggatg gcttcttgct tacgcagtgc gattaacttt tggtaggtgt aaaacaccga
    34381 ggactcatcg gccagcgcgg cttctacgtt gatttgttga tagttatcgc ccaggccaat
    34441 ccacggttcg ccagccgtaa acccggcatt atcgccgttg ctccattgca tgggcgtgcg
    34501 gctgttgtca cgggatttac tggcgaggat tgccaataac tcgtcggcat cacgaccatc
    34561 gttgcgcagc tcggcaaaca tattgaggct ctccacgtcg cgatagtcag taatgcgcgt
    34621 gaaatgcggg ttggtcatgc caatctcttc gccctggtag atatacggcg ttccctgcat
    34681 gccatgcagc accatcgcca gcatttttgc cgcaggcacg cggtattcac cttcatcacc
    34741 aaagcgagaa acaatgcgcg gctgatcgtg gttacaccag aacaaggcat tccatgccac
    34801 gttgtgcatt ccttgttgcc agtggcggaa caatgttttc aacgccacaa agtcaggttt
    34861 agccagcgtc catttttcac caccgggata atcgaccttc aggtgatgaa aattaaaggt
    34921 catcgacaat tcactgcctg tcagtgccgc gtatcgctgg caatgctcaa ggctggtgga
    34981 ggacatttca cctacggtca ttaacccgcg tggcgtaaac acatcgcggt tcatctcgtg
    35041 caaaaactcg tgtgctcgtg gcccgtcggt gtagaagcga cgcccgtcgc catccaggtc
    35101 ttcagggaaa cgcgggtctt tggagatcag attcaccaca tccaggcgca acccgtcgac
    35161 cccacgatcg gcccagaatt cacagacttt tttcagctct gcgcgtaccg ctggattctc
    35221 ccagttgaga tccgcctgtt ctggtgcaaa gagatgcaaa tagtactgtt cgctttccgc
    35281 atgccagcgc cacgcactac cgccaaattt tgaacgccag ttgttcggtg gcgtttctgg
    35341 ttcgccatcg cgccagatat aaaactggcg gtaagggctt tctttgttca gcgcctcgcg
    35401 aaaccaggca tgttgggtag aggtatggtt aaacaccata tcgagaatga tacgaatccc
    35461 gcgcgatttt gcctgcgtca ccagttcgtc aaaatcgtcc agcgtgccgt agatgggatc
    35521 aatcgccgta tagttcgcta cgtcgtaacc gttatcgacc tggggagaga tataaaaggg
    35581 ggttagccag atggcatcaa cgcccagttt atgcagatag tccaggcgtt ggataacgcc
    35641 acgtaaatcg ccggtgccgc tacccgtggt gtcctgaaaa ctctttggat aaatctggta
    35701 gataacgccg ttttgccacc agtggggaag attagtcatt acgttattcc tgcaaatgcg
    35761 agggggcgca attgcgcccc gaagaaaatt aaacaatatc cagcgtgccc aggcggtatt
    35821 tccgctgata gataaacgag gtgagtacaa tcgggatgat gatggcgata accattgcca
    35881 gcgcaaatac ctgccagtag ctcggttgaa tcgagagaat tcccggcagg ccgcctacgc
    35941 cgataccatt cgccataacg ccgttcaggc cgcatagtaa tcctgccaga ccagaaccaa
    36001 tcatcgcgca cagcatcggg aagcgatatt tcaggttgat gccgtacatt gcaggctcag
    36061 tgaccccaag ccaggcggag atagcggcag gcacggagat ctcgcgttca ttgtgcttgc
    36121 ggctggaaat gataatgcct atcacggcgg agccctgagc gatattcgac agcgcaatca
    36181 gcggccacac tggcgtgcca cccatgcttt gaatcatctg caaatcaatc gcaagggtgg
    36241 tctggtgtac accggtgatc accagcgggg cgtacaggaa gccaaacaat gctgcgccaa
    36301 tcggagcaaa gctgccggtc atcaggtgac gtaccgcaaa ggcaacgcca tcgccaatca
    36361 tgcgaccaaa cggaccaatc agcgcatggg cgaggaacac cgcgaggatc agcgaacaga
    36421 cgggcaccac caccagatag aggtaatccg gcacgatgcg tttaaggcga gtttcaataa
    36481 cgcccagcgc cagcccggct aacagtgccg ggatcacctg cgcctgatag cctactttgg
    36541 cgatgctgaa catgccaaag tcccacactt ccggcagctg ctgcccgagc agataagcgt
    36601 tcatcagctg gggagaaacc agtgtcacgc caagcacgat accaaggatc ggcgtgccgc
    36661 ccattttttt caccgctgac cagcaaatcc cgaccggcag gtagaagaag atcgcttcac
    36721 cgatcaacca cagaaaatcg tagatcgttt gcagggaagg gtacatttgc gccagcgttt
    36781 gaccgttgct catgggcaaa tcgccgatca cattgcgaaa accgaggatc aaaccgccgc
    36841 taatcaacgc gggcagcaac gggaagaaga tctccgcgaa atgagagatc aactgctcat
    36901 gccatttcat attctgccgg gcggcttttt ttacctgctc tttatcaacc tgcgcctgtc
    36961 cggttgacgc tattagtgct tgatagtaat cacccacgtt ggtgccaatc accacctgaa
    37021 attgcccggc attggtgaaa cagcctttca ccatggggag ttgctcaatt tctttcggtc
    37081 tggcattggc cggttggttg aggacaaagc gcaggcgagt aatacagtgg ctcaccgtcg
    37141 caatattgcc gcgcccgccg accagttcaa tcaaccgatc gatatccgtt tggtttattt
    37201 tgctcatcat aaagccccat ggcagatgac atttttggtt ggctgcagaa tatattgcgc
    37261 ggaaaaattt aaaaacggga acgttcccga aacgcagcga agatcacaat ttatcgttca
    37321 ggaaacgatc aggacagggt ggcggggatg atgatttgtt gcggttcgct gcgcccggtt
    37381 acctgtgcga tcaattggca ggccgcctgg cgtccagctt cggcgtaacc aggatctacg
    37441 gtgacgatct ccggatgaag gaatttcatt aatggcgtat taccgacgct cgccagttgc
    37501 aaggtgtcga tgcgttgctc ttgcaggtat ttacttgcgc caagtgccag cgtgtcggtt
    37561 gcgcacagta aggcggtagt ttcaggcgta atcacttttg ctacgttttc atagccttgc
    37621 ttcatagcaa gccctggcag agcggcaacg ggatgcagtt tatgcgcttt gcagaacgcc
    37681 aggtaggctt cgtgacgtcg cttcccggtc gtcacgtcac tatgcggcac gccgagataa
    37741 ctgatattgc gatgcccctg gtcatagagc cgttgcatca ggatcttgat tgccccttcg
    37801 tcgtcataac agaccgaagc aaagcctttt gcgtcacgcg ccagcagaac cagcgatgac
    37861 tgccagtggg ctaacatttc ttctgttatg ccagtaaaac cgaacaacac tacgccgtcg
    37921 atattacgcc gtttcagcac ccccaaatgt tcggcaacta attgcgggga aaactgactt
    37981 tccatcatga ttgggtcgta accttgttca tagaacgctg gcagcatggt ttgaacagcg
    38041 agattttctg acaacgaatc caggcgggta acaatgatgg cgaccacttt atcgctttgc
    38101 ccacgcatag cgcgcgcaga gcgggaaggg gaaaatccat gctgattcat cactgcttca
    38161 acacgctcgc gggtgcgctg gctcacgccg ctttcgttat tcagcacccg ggaaaccgta
    38221 gatttcccca cgccgcttaa gcgcgcgatg tctttgatgg tcagccgatt ttgcatcctg
    38281 ttgtcctgta acgtgttgtt taattatttg agcctaactt tacccgtgca ttcagcaatg
    38341 ggtaaagtct ggtttatcgt tggtttagtt gccagcaggt attatatcgc catagatgct
    38401 acgaatatta ttggattctc cttattattt gcggcgcttt tttcacttac cggaggttat
    38461 atggaacctg atcccacgcc tctccctcga cggagattaa aacttttccg gtaagcccgt
    38521 cttttcacgg cgttaccgga tgcgtaaggc cgtgacgttt taacgtccct gctcagcttt
    38581 attaccttca ggtaaggctt cgccacgcct gaagacattt ctgtactgtt tcagacagtg
    38641 cggagggact ccttatgttt aaagaaattt ttacccggct cattcgccat ttaccttccc
    38701 gtctggttca tcgcgatcca ttgcctggcg cgcagcagac agtgaatacg gcggtcccgc
    38761 cgtccttaag tgcgcattgc ctgaaaatgg cggtgatgcc cgaagaagaa ttatggaaaa
    38821 cgttcgacac ccatccggaa gggttaaatc aggcggaagt ggaatctgcc cgcgaacaac
    38881 atggtgaaaa taaattaccc gcacaacaac cgtcgccgtg gtgggtacat ttatgggtct
    38941 gctatcgcaa cccctttaat attttactca ccattctcgg cgctatttct tacgccacgg
    39001 aagatttatt tgccgcgggc gttattgcgc taatggtcgc tatttcaacg ttactgaact
    39061 ttattcagga agcacgttcc actaaagcgg cagatgccct gaaagcgatg gtcagcaata
    39121 ccgcgacggt gctgcgcgta attaacgaca aaggcgaaaa tggctggctg gagatcccga
    39181 tcgaccagct ggtgcccggc gatattataa aactggcggc gggagatatg atcccggcag
    39241 atttacgtat cttgcaggcg cgggatctgt tcgtcgctca ggcgtcgtta accggtgagt
    39301 ctctgcccgt ggaaaaagcc gctaccactc gccagccgga gcacagcaat ccgctggagt
    39361 gcgacacgct gtgttttatg ggcaccaccg tggtgagcgg cacggcacaa gcaatggtga
    39421 ttgctacagg tgccaatacc tggtttggtc aactggcggg gcgtgttagt gagcaggaaa
    39481 gcgagccgaa tgcctttcag caggggatca gccgcgtcag tatgctgctg attcgcttta
    39541 tgctggtgat ggctccggtg gtgttgctta tcaacggtta caccaaaggc gactggtggg
    39601 aagcggcact gtttgcgctt tcggtcgcgg taggcttaac gccggaaatg ttgccgatga
    39661 ttgttacctc gacgctggcg cgcggcgcag taaagctgtc gaaacagaaa gtgatcgtca
    39721 aacatctgga tgctattcag aactttggcg caatggatat tctgtgcact gataaaactg
    39781 gcaccctgac acaggacaaa attgtgctgg agaatcatac cgatatctcc ggtaaaacca
    39841 gcgaacgcgt gctgcatagc gcgtggttga acagtcatta tcagaccgga cttaaaaacc
    39901 tgctcgatac agcggtgctc gaaggtacgg atgaagagtc agcgcgctcg ctggccagtc
    39961 gttggcaaaa aattgatgag attccgtttg atttcgagcg tcgccggatg tcggtggtag
    40021 tggcagaaaa taccgagcac catcagctgg tttgcaaagg tgcattgcag gaaatcctca
    40081 atgtgtgttc gcaggtgcgt cacaatggcg agattgtgcc gctcgatgac atcatgctgc
    40141 gtaagattaa gcgggttact gatacgctta atcgtcaggg gctgcgcgtg gttgcggtgg
    40201 cgacgaaata cctgccagcg cgtgaaggag attaccagcg ggcggatgaa tccgacctga
    40261 tcctcgaagg atatattgct ttccttgatc cgccaaaaga gacaaccgct ccggcactga
    40321 aggcattaaa agcgagtgga atcaccgtaa aaatccttac tggcgacagt gagttagttg
    40381 cagcgaaagt gtgccatgaa gtggggctgg acgcgggaga ggtggtcatt ggtagtgata
    40441 ttgaaacgct atctgacgac gaactggcaa atctcgcaca gcgcaccacg ctgtttgccc
    40501 gcctgacgcc gatgcataaa gaacgcattg tgaccttact gaagcgcgaa gggcatgtgg
    40561 ttggctttat gggcgatggt attaatgatg cgcccgcctt acgcgctgcg gatatcggca
    40621 tttctgtgga cggcgcggta gatattgccc gtgaagcggc tgatatcatc ctgctggaaa
    40681 aaagcctgat ggtgctggaa gagggggtta ttgaaggacg tcgcactttc gccaacatgc
    40741 tgaaatacat caaaatgacg gcgagctcca acttcggtaa tgtgttcagc gtgctggtgg
    40801 cgagtgcttt cttgcccttc ctgccgatgt tgccgttaca cttgcttatt cagaacctgc
    40861 tgtatgatgt gtcacaggtg gcgatcccgt ttgataacgt cgacgacgag caaattcaaa
    40921 agccgcagcg ttggaatccg gcggatctgg ggcgctttat gatcttcttc ggaccgatca
    40981 gctcgatctt cgatattctg acgttttgcc tgatgtggtg ggtattccat gccaacacgc
    41041 cggaaacgca aacgctgttc cagtcgggat ggtttgtggt gggcttactg tcgcaaacgc
    41101 tgattgtgca tatgatccgc acccgccgtg tgccgtttat tcagagctgt gcatcgtggc
    41161 cgttaatgat catgaccgtg atcgtgatga ttgtcgggat cgcattgccg ttttcaccgc
    41221 tggccagtta tctgcagctg caggcgctgc cgttaagcta tttcccgtgg ctggttgcga
    41281 ttctggcagg gtatatgaca ttaactcagt tggtgaaagg attttatagc cgtcgttacg
    41341 gctggcagta aagaataaac tgggcacgat agccccgatt cagattgcag acaaagtaag
    41401 ctttgttcat gccggatgcg gcgtaaatgc cttatccggc caacaaattc aataaattgc
    41461 aggaatcacg taggcctgat aagcgtagcg catcaggcga ttttgcgttt gtcatcagac
    41521 ttaatccggg catgatagcc cggatttcca tcaagattag cgacgaacag cgatcgcttc
    41581 gatctcaatc ttcacgtctt tcggcagacg ggcaacttca acgcaagaac gtgccgggaa
    41641 ggtggcattg tgttcggtga agaaggcttc gtaggtagca ttaacggttg cgaagtcgtt
    41701 cagatctttc acaaacacgg tggttttaac gatgtcgccc actttcaggc ctgcggcttc
    41761 gacgatcgct tttacgttat ccagcgactg acgtgcctgt gcagcgacgt ctgccggtac
    41821 ttcgcccgtt ttcggattta ccgggatctg gccggaggtg atgatcatat tgcccagatc
    41881 aacgccctgt acgtaaggac cgatagctgc cggtgcattt tccgtcgcga tagttttgct
    41941 catgatttct cctttattac agcggtaaaa agtctggctc attataggga gccagacttt
    42001 tattaccaac cgcaattaat tggccagcac cacattatgg gaaaactctt tttcacagta
    42061 tttgcatttg agcgcgatgt cattggcgcg ttttcgcacg gcaaagctgg aagaaaccgg
    42121 ttcggcatgg ctgatacagt tgctgttcgg gcagaccagc acattgtcga tgcgctctgg
    42181 cagactgggg cgcgatttac ccaccacttc atagttgtcg atacggttaa ccgtggcttg
    42241 cggcgcatac aatgccagtt gatctacctg atcttcactc aaaaaggtat tttcgatttt
    42301 gatcagatct ttgcggccca tctcgccaga aggcaagttc agaccgatgg tgatgcgctg
    42361 atccgtttcg gtcagcttga acagactcaa cagcttaaaa ccgatctggg cggggatatg
    42421 gtcaattacc gtgccgcgtt taatagcttc aacctgcaat ttattatcgt gtgtcatctc
    42481 tatttcccct tacagtacca gatcgcgatt cagaaccagt gccagtaacg cctggcgagc
    42541 gaaaatcccg ttgcctgcct gctggaagta ccaggcgtgt ggcgttttat caacatccgt
    42601 cgcaatctca tcaacacgcg gcagcggatg cagcactttc atattggctt tggcgttgtg
    42661 gagatcgctg gcgcgaagaa caaactgcgc tttcacgttg gcgtactcgg acgggtccag
    42721 acgctctttt tgcacgcggg tcatgtacag gatgtctact tccgctatca cttcttcaat
    42781 agagctgtgc agactccatg cgatcccttt ttcatcgagc atatccagaa tgtattgcgg
    42841 cattgccagc gcgtccggcg cgatgaagta aaaacggttg ccgtcgaact tcgctaacgc
    42901 ctgagtcagg gagtgaacgg tgcggccata tttcaggtca ccaaccattg cgacgtggag
    42961 attgtccaga cgcccctggg tttcctgaat agtgaataag tccagcaagg tttgcgtcgg
    43021 atgttggttg gagccatcac cggcattcag taccggtaca ttgccggaaa actcggtggc
    43081 caggcgcgcc gcaccttcct gcggatgacg catcactatc gcatcgacgt aagtgctgat
    43141 aaccgaaatg gtatcggcca gcgtttcgcc ctttttaccc agtgatgtat tggcgctgtc
    43201 ggagaagccc actacgctgg cacccaggcg gtgcatagat gtttcgaaag agaggcgggt
    43261 acgggtagag gcttcgaaga aacagctggc aatgactttg tgcttcaaca gctctggttg
    43321 cgggtttgct ttcagtttcg ccgctgtcgc cagcaccaga ttaaggtcat cgcgactaag
    43381 gtcgtttatg gaaatgatat gtttctgata tagcggatta gccatctttt atctcctgac
    43441 gcctgggcaa aaaaaagccc ctcgattgag gggctgggaa tgggtgatca acgggaagaa
    43501 aaacggcagg ccagcgtctt ttttcagacg cggtaagaca aaatgtcgaa cacactgaac
    43561 catacatcct cccggcaaat tgtccggcat tatactcatc gtcagaagcg gtgcaagcat
    43621 ttgatgcaat attttgtcag cgcaaacggt ttatttgaat tataagtcaa aatacatgca
    43681 tttttatttg ttgtgattct gcaggggaac ggtcgttcgg tatcaataat tgcagacatt
    43741 tcctgctttt ttctttttca ctttcacgca atcagatatg cattttattc cattcatccg
    43801 acttataggg cggagtgtga aaacgaacgg ctaacactat tgcttactgc tcagggatgc
    43861 gcgctatcac tttaatttca aaatcaaagc ctgccagcca tgtaacacct accgccgtcc
    43921 agtttggata aggtggggcg ctaaatattt catttttcac cgtcatgatg tcttcaaatt
    43981 ggttttctgg atcggtatgg aagctcgtaa catcaatgat atcgtcaaaa gtgcatcccg
    44041 cagcagccag ggtcgcatgc aaattatcaa atgccagtct gacttgttgc tgaaaatcgg
    44101 gttctggtgt tccgtcctct cgacttccta cttgcccgga aacaaacagc aaatcgccgg
    44161 agcgaatagc cgcagaataa cgatgttcag catacagtga atgtcggcca gcagggaaaa
    44221 cagcggttct ttctaccatt tggttatcct caagatttac gacatgaaca gaagatttat
    44281 atttaccagg agccgctttt agcggacgac gtgagtaaac aaaacccaga catcatggga
    44341 aatggctggg cttaattgag cgtagtcggt tatgcgccaa acgcgccatc aatggtatgc
    44401 atcgcgccgg taacaaagct ggcttctggc cctgctaacc atgcgaccat accagcgacc
    44461 tcttccggtt gcccatgtct tttgatagcc ataaaaccat gcaacatatc gcgcattggc
    44521 ccgttggcgg gattagcgtc ggtatcaatt ggccctggct ggacgacgtt gatggtgatc
    44581 ccacgcggtc caaaatcacg ggccagcccg cgcgccatgc cttgcagggc agatttgctg
    44641 gcggcataag cagccatgcc tgcaacaggc atacgatcgc cattcacgga gccgatgatt
    44701 aagatgcgcc cgccttcggg catctgccgg gcggcttcaa cggaggcatg gtaaggagca
    44761 tgaatattga ttttgaaaag gcgatcaata tcgtcggcat ttaattccag ggcatcgcca
    44821 aagacgccaa tacctgcatt taccacgtgg atatccaatg cgccgctctt acgaacgaca
    44881 tcaataacag cgtctctgtc agcactatct gtgaatactg ctgtcgctcc cgtctcttgt
    44941 gccaggcgtt cagcggcatc tttcgacccc gcataggtga atcgtacatt ggccccatcg
    45001 gtgacgaaac gacgtacgat agcggcaccg attccacggc taccaccgag gatgagaact
    45061 gtcttacctg taaaagcgcc cataaggact ccttgattta ttatgtaaca tgcattacaa
    45121 aactgtttta actttctgtc aacaagtttt gtaatggtca ctaaaaaaca atctcgcgtt
    45181 ccaggtcgtc ccagacgttt cgctcctgag caggcagtct ctgcggcaaa agtgcttttt
    45241 caccaaaaag gtttcgatgc tgtcagtgtt gctgaagtta ctgattatct tggtattaac
    45301 cccccgagcc tctacgcggc ttttggcagt aaggctgggt tatttagccg tgtactcaat
    45361 gaatatgtcg gtacggaagc tattccgctt gccgatattc ttcttgatga tcgtccggta
    45421 ggcgagtgcc tggttgaggt attaaaagaa gcggcgcgca gatatagcca aaacggcggc
    45481 tgcgctggct gtatggttct tgaaggtatt catagtcatg atccacaagc gcgtgatatt
    45541 gccgttcaat attatcacgc cgcagaaacg accatttatg actatatcgc caggcggcat
    45601 ccacaaagcg cacaatgtgt gactgatttt atgagtaccg tgatgtcagg gctttctgcg
    45661 aaggcacggg aggggcactc catcgaacaa ctctgtgcaa cagctgcact ggcgggggaa
    45721 gcgataaaaa ctattctcaa ggagtgatgc gggccttgac ccgaaaggcg ggaacgcgcc
    45781 tgccgataag ttgtgataag acaataattc acgcgttaag gctagcggaa ttgatcatct
    45841 tttcgtataa cgatagaaat gaaacgttgt tttaattaag gagtggaaaa gatgatcatc
    45901 ggaaatattc ataatcttca gccgtggcta ccccaggagt tacgccaggc gattgagcat
    45961 atcaaagcac acgttacggt agaaacgcca aagggcaagc acgatatcga aggcaatcgt
    46021 ctgttttatc ttatctcgga agatatgacc gagccgtacg aagctcgccg tgcggagtac
    46081 catgcccgct atctcgacat tcagattgtg ttaaaaggtc aggaaggcat gaccttcagc
    46141 acacaacctg caggtacgcc ggataccgac tggttagctg ataaagacat cgcatttttg
    46201 ccggaaggcg ttgatgagaa aacagttatc cttaatgaag gtgattttgt tgtgttttat
    46261 ccgggggaag tgcataaacc gctgtgcgca gtgggtgcac cagcccaggt tcgcaaagca
    46321 gtagtgaaga tgctgatggc gtgatgactt ttcgccgtaa ataactccag gtttacggcg
    46381 agtttgtgaa aagagcgttt tttgatattt tttgtgagta aaatttgtaa tgcttagacg
    46441 ttcttattaa ctcaaggagt ccgtcatgag caaaatatca ggttggaatt tttctcaaaa
    46501 cattacatca gccgacaatt gtaaacaaaa aaatgaagac ttagatacct ggtatgtggg
    46561 aatgaatgat tttgcccgaa ttgccggagg gcagaatagc agaagcaata ttctttctcc
    46621 cagagcattt ttggagtttt tggctaagat atttaccctg ggttatgtgg attttagcaa
    46681 acgctccaac gaagcgggta gaaatatgat ggctcataat aagtcctcat cttatatcaa
    46741 aaataatgat ggtagtgaga taatgaagtt tgttatgaat aatcctgaag gggagcgagc
    46801 ggatttatct aaggtggaaa tagaaattac acttgcctca gcttttaata atgggattcg
    46861 tgaaggacat acagtcatta tatttacaca acctgatggt tcgactaatc gttatgaagg
    46921 gaagtccttt gaaagaaaag atgagagttc attacacctg attactaaca agattctggc
    46981 gtgttaccaa cgcgaagcta acaaggaaat agctcgtcta ttaaatattc cgcaggagtt
    47041 aaataattct caggacttga ataactcgca ggtgagttgt aaagattcag ttgattctac
    47101 gattacggat ttattagaaa aaccattgaa taatgcatta ttagcaataa ggaaagaaca
    47161 tctgctatta atgccttatg tatgtaatga atcgatttca tacttgcttg gtgaaaaaga
    47221 gatactgaaa gaaatagatg accttaacgc agtaaataat tatcttctta ataataagaa
    47281 agctactgac aatgaaa
//
LOCUS       DAZGVQ010000018        45892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000018 DAZGVQ010000000
VERSION     DAZGVQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.018"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            273..899
                     /locus_tag="ACHLAG_000988"
     CDS             273..899
                     /locus_tag="ACHLAG_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFP0654266.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            920..3022
                     /locus_tag="ACHLAG_000989"
     CDS             920..3022
                     /locus_tag="ACHLAG_000989"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFP0654267.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            3035..3673
                     /locus_tag="ACHLAG_000990"
     CDS             3035..3673
                     /locus_tag="ACHLAG_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFP0654268.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3737..4405
                     /locus_tag="ACHLAG_000991"
     CDS             3737..4405
                     /locus_tag="ACHLAG_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP0654269.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4402..5187
                     /gene="phsC"
                     /locus_tag="ACHLAG_000992"
     CDS             4402..5187
                     /gene="phsC"
                     /locus_tag="ACHLAG_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFP0654270.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5191..6003
                     /gene="ydhT"
                     /locus_tag="ACHLAG_000993"
     CDS             5191..6003
                     /gene="ydhT"
                     /locus_tag="ACHLAG_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFP0654271.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(6015..7619)
                     /locus_tag="ACHLAG_000994"
     CDS             complement(6015..7619)
                     /locus_tag="ACHLAG_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFP0654272.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYSVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7745..8050)
                     /locus_tag="ACHLAG_000995"
     CDS             complement(7745..8050)
                     /locus_tag="ACHLAG_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFP0654273.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTVRLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8158..8234)
                     /locus_tag="ACHLAG_000996"
     tRNA            complement(8158..8234)
                     /locus_tag="ACHLAG_000996"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8198..8200),aa:Val,seq:gac)
     gene            complement(8239..8315)
                     /locus_tag="ACHLAG_000997"
     tRNA            complement(8239..8315)
                     /locus_tag="ACHLAG_000997"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8279..8281),aa:Val,seq:gac)
     gene            8623..9879
                     /locus_tag="ACHLAG_000998"
     CDS             8623..9879
                     /locus_tag="ACHLAG_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFP0654274.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9920..11293)
                     /gene="mdtK"
                     /locus_tag="ACHLAG_000999"
     CDS             complement(9920..11293)
                     /gene="mdtK"
                     /locus_tag="ACHLAG_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFP0654275.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            11508..12149
                     /gene="ribE"
                     /locus_tag="ACHLAG_001000"
     CDS             11508..12149
                     /gene="ribE"
                     /locus_tag="ACHLAG_001000"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFP0654276.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12189..13337)
                     /gene="cfa"
                     /locus_tag="ACHLAG_001001"
     CDS             complement(12189..13337)
                     /gene="cfa"
                     /locus_tag="ACHLAG_001001"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFP0654277.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13628..14839)
                     /gene="punC"
                     /locus_tag="ACHLAG_001002"
     CDS             complement(13628..14839)
                     /gene="punC"
                     /locus_tag="ACHLAG_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFP0654278.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14952..15884
                     /gene="punR"
                     /locus_tag="ACHLAG_001003"
     CDS             14952..15884
                     /gene="punR"
                     /locus_tag="ACHLAG_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFP0654279.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15881..16906)
                     /gene="purR"
                     /locus_tag="ACHLAG_001004"
     CDS             complement(15881..16906)
                     /gene="purR"
                     /locus_tag="ACHLAG_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFP0654280.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17205..17294
                     /gene="cydH"
                     /locus_tag="ACHLAG_001005"
                     /gene_synonym="ynhF"
     CDS             17205..17294
                     /gene="cydH"
                     /locus_tag="ACHLAG_001005"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFP0654281.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17460..18629
                     /locus_tag="ACHLAG_001006"
     CDS             17460..18629
                     /locus_tag="ACHLAG_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0654282.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(18775..19356)
                     /gene="sodB"
                     /locus_tag="ACHLAG_001007"
     CDS             complement(18775..19356)
                     /gene="sodB"
                     /locus_tag="ACHLAG_001007"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFP0654283.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19484..20299)
                     /gene="mepH"
                     /locus_tag="ACHLAG_001008"
     CDS             complement(19484..20299)
                     /gene="mepH"
                     /locus_tag="ACHLAG_001008"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFP0654284.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20634..20981
                     /gene="grxD"
                     /locus_tag="ACHLAG_001009"
     CDS             20634..20981
                     /gene="grxD"
                     /locus_tag="ACHLAG_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFP0654285.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21032..25648)
                     /locus_tag="ACHLAG_001010"
     CDS             complement(21032..25648)
                     /locus_tag="ACHLAG_001010"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFP0654286.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASGTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDTAHFE
                     STSGATSNHVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(25741..26388)
                     /gene="rnt"
                     /locus_tag="ACHLAG_001011"
     CDS             complement(25741..26388)
                     /gene="rnt"
                     /locus_tag="ACHLAG_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFP0654287.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26491..26898)
                     /gene="gloA"
                     /locus_tag="ACHLAG_001012"
     CDS             complement(26491..26898)
                     /gene="gloA"
                     /locus_tag="ACHLAG_001012"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFP0654288.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGHGLGN"
     gene            complement(26979..28076)
                     /gene="nemA"
                     /locus_tag="ACHLAG_001013"
     CDS             complement(26979..28076)
                     /gene="nemA"
                     /locus_tag="ACHLAG_001013"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFP0654289.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28113..28712)
                     /gene="nemR"
                     /locus_tag="ACHLAG_001014"
     CDS             complement(28113..28712)
                     /gene="nemR"
                     /locus_tag="ACHLAG_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFP0654290.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            28815..29054
                     /locus_tag="ACHLAG_001015"
     CDS             28815..29054
                     /locus_tag="ACHLAG_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFP0654291.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPKQPSLF"
     gene            29103..29999
                     /locus_tag="ACHLAG_001016"
     CDS             29103..29999
                     /locus_tag="ACHLAG_001016"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFP0654292.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            30080..30601
                     /gene="sodC"
                     /locus_tag="ACHLAG_001017"
     CDS             30080..30601
                     /gene="sodC"
                     /locus_tag="ACHLAG_001017"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFP0654293.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30602..32614)
                     /locus_tag="ACHLAG_001018"
     CDS             complement(30602..32614)
                     /locus_tag="ACHLAG_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP0654294.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNTEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICNSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32614..33471)
                     /locus_tag="ACHLAG_001019"
     CDS             complement(32614..33471)
                     /locus_tag="ACHLAG_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFP0654295.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33474..33710)
                     /locus_tag="ACHLAG_001020"
     CDS             complement(33474..33710)
                     /locus_tag="ACHLAG_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFP0654296.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33911..34345
                     /gene="slyA"
                     /locus_tag="ACHLAG_001021"
     CDS             33911..34345
                     /gene="slyA"
                     /locus_tag="ACHLAG_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFP0654297.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34392..34859)
                     /gene="slyB"
                     /locus_tag="ACHLAG_001022"
     CDS             complement(34392..34859)
                     /gene="slyB"
                     /locus_tag="ACHLAG_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFP0654298.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSTGQRVVLASNGSQVTVSPR"
     gene            35133..36242
                     /gene="anmK"
                     /locus_tag="ACHLAG_001023"
     CDS             35133..36242
                     /gene="anmK"
                     /locus_tag="ACHLAG_001023"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFP0654299.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36340..36669
                     /gene="mliC"
                     /locus_tag="ACHLAG_001024"
     CDS             36340..36669
                     /gene="mliC"
                     /locus_tag="ACHLAG_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFP0654300.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36728..37384
                     /gene="pdxH"
                     /locus_tag="ACHLAG_001025"
     CDS             36728..37384
                     /gene="pdxH"
                     /locus_tag="ACHLAG_001025"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFP0654301.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37513..38787
                     /gene="tyrS"
                     /locus_tag="ACHLAG_001026"
     CDS             37513..38787
                     /gene="tyrS"
                     /locus_tag="ACHLAG_001026"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFP0654302.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38846..39709
                     /gene="pdxY"
                     /locus_tag="ACHLAG_001027"
     CDS             38846..39709
                     /gene="pdxY"
                     /locus_tag="ACHLAG_001027"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFP0654303.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39753..40358)
                     /gene="gstA"
                     /locus_tag="ACHLAG_001028"
     CDS             complement(39753..40358)
                     /gene="gstA"
                     /locus_tag="ACHLAG_001028"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFP0654304.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     VVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40464..41966)
                     /gene="dtpA"
                     /locus_tag="ACHLAG_001029"
     CDS             complement(40464..41966)
                     /gene="dtpA"
                     /locus_tag="ACHLAG_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFP0654305.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(42579..43214)
                     /gene="nth"
                     /locus_tag="ACHLAG_001030"
     CDS             complement(42579..43214)
                     /gene="nth"
                     /locus_tag="ACHLAG_001030"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFP0654306.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(43214..43909)
                     /gene="rsxE"
                     /locus_tag="ACHLAG_001031"
     CDS             complement(43214..43909)
                     /gene="rsxE"
                     /locus_tag="ACHLAG_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFP0654307.1"
                     /translation="MSEIKDVIVQGVWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATRAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDKRMKKRRAEAT
                     AERALPNGETGNV"
     gene            complement(43913..44533)
                     /gene="rsxG"
                     /locus_tag="ACHLAG_001032"
     CDS             complement(43913..44533)
                     /gene="rsxG"
                     /locus_tag="ACHLAG_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFP0654308.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQEA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(44537..45595)
                     /gene="rsxD"
                     /locus_tag="ACHLAG_001033"
     CDS             complement(44537..45595)
                     /gene="rsxD"
                     /locus_tag="ACHLAG_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFP0654309.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(45596..>45892)
                     /locus_tag="ACHLAG_001034"
     CDS             complement(45596..>45892)
                     /locus_tag="ACHLAG_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654310.1"
                     /translation="EPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAA
                     VEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT        11546 a        11344 c        11968 g        11034 t
ORIGIN      
        1 tatttcaaat gataataaat atcagttcac ttataatttc ttgctttgta attttaattg
       61 ataacgatca atgttaaggg ctcagaataa tcacattatt ttttctccta accataaagg
      121 attagtttat cggcgttaaa tcttttctca tacactatcg ggttagggaa aatatctggt
      181 gttggggaca gagtcagcag cgtattagtt gaatgcttaa tatcaggctg ctgaattgtc
      241 agcactgtgt ttgcgaagag gatgcgaaat aaatgaaccc ggttgatcgt ccactattag
      301 atattggctt aacgcgatta gaattcttgc gtatatcagg aaaaggcctt gctggtctaa
      361 caattgcccc tgcgttactg tcacttttgg gttgcaaaca ggaagatatt gatagcggca
      421 cagtagggtt gataaataca cccaaagggg tgctggtgac ccagcgagcg cgatgcacgg
      481 gttgccaccg ctgtgaaatc tcttgtacca acttcaacga tggctcagta gggacattct
      541 tctcccgtat caaaatccac cgcaattatt tctttggcga caacggggtt ggctctggcg
      601 gcggcctgta tggcgatctc aactataccg cggacacctg ccgtcaatgc aaagagccgc
      661 aatgcatgaa cgtctgcccg attggtgcga ttacctggca gcagaaagaa ggctgtatta
      721 ccgtcgatca taaacgctgt attggctgta gcgcctgtac tacggcatgc ccgtggatga
      781 tggccaccgt aaataccgaa agtaaaaaat cctcgaaatg tgtgttatgc ggtgaatgcg
      841 caaacgcctg cccaacaggg gcgttaaaaa ttatcgagtg gaaagatatt actgtgtgaa
      901 tcttgcaaag gaaaaaatca tggctaacgg ttggacaggt aatatattaa gagtcaatct
      961 cacgacagga aatattaccc tcgaagattc cagtaagttt aaaagttttg tcggtggcat
     1021 gggcttcggc tacaaaatta tgtatgacga agtaccgcca ggcacgaaac ctttcgatga
     1081 agcgaataaa ttagtctttg ctaccggccc attaactgga tctggtgccc cctgtagttc
     1141 tcgcgtaaat atcacctcac tttctacttt taccaaagga aatttagttg tcgatgccca
     1201 tatgggtggc ttttttgcag cgcaaatgaa attcgctgga tacgacgtca ttattatcga
     1261 agggaaggcg aaatcaccgg tatggctgaa gattaaagat gacaaagtta gcctggaaaa
     1321 agccgatttc ttatggggaa aagggacgcg cgcaacgacg gaagaaattt gtcgattgac
     1381 cagtccggaa acctgtgtgg cggctattgg tcaggctggg gaaaaccttg ttcctctctc
     1441 tggcatgttg aatagccgta accacagcgg cggtgcggga actggcgcaa ttatgggttc
     1501 gaaaaacctg aaagcgattg cggttgaagg gacgaaaggg gtcaacattg ccgatcgtca
     1561 ggagatgaag cgtctcaacg attacatgat gactgaactt attggtgcga ataacaacca
     1621 tgtcgtgcca agtacgccgc aatcgtgggc agagtattca gatcccaagt cacgttggac
     1681 agcacgtaaa gggctgtttt ggggcgcggc tgaaggtggt ccgattgaaa cgggggaaat
     1741 tccgccaggc aatcagaaca cggtcggctt tcgtacctat aaatccgttt ttgacttagg
     1801 tccggcggca gagaaataca cagtaaaaat gagtggctgc cactcttgcc cgatccgttg
     1861 tatgacccaa atgaatattc ctcgggtgaa agagtttggc gtgcccagca caggtggtaa
     1921 cacttgtgta gccaactttg tccataccac catcttcccg aacggtccga aagattttga
     1981 agataaagac gatggccgtg tgattggtaa cctggtgggt ctgaatttgt tcgatgacta
     2041 cggcctatgg tgtaactacg ggcagttgca tcgcgacttt acctattgtt acagcaaagg
     2101 ggtgttcaag cgtgttctgc ctgctgaaga gtatgcggaa attcgctggg atcaactgga
     2161 agcgggtgac gttaacttca ttaaagattt ttactaccgt ctggcgcatc gtgtgggtga
     2221 gctgagtcac ctggctgatg gttcatatgc catcgcagaa cgctggaatt tgggtgaaga
     2281 gtactggggc tacgcgaaaa ataaactctg gtcgccgttt ggctatccgg ttcaccatgc
     2341 caatgaagcg tcagcgcagg tcggttccat tgttaactgt atgttcaacc gtgactgcat
     2401 gacgcatacc catatcaatt ttattggttc gggtttgcca ttgaaactgc agcgtgaagt
     2461 ggcgaaagag ctttttggtt ctgaagatgc ctacgatgaa accaaaaact acacgccaat
     2521 caatgacgca aaaattaaat atgcaaagtg gtcgctgttg cgggtctgtt tgcataacgc
     2581 cgtcaccctg tgcaactggg tctggccaat gaccgtttcg ccgctgaaaa gccgtaatta
     2641 ccggggcgat ctggcgcttg aagccaaatt cttcaaagcg atcaccggcg aagagatgac
     2701 tcaggaaaaa ttagatttag ctgcagagcg tatctttacg ttgcatcgtg cctacacggt
     2761 aaaactgatg caaaccaaag atatgcgtaa tgaacacgat cttatctgtt cctgggtatt
     2821 cgacaaggat ccgcagatcc cggtctttac tgaaggtact gacaaaatgg atcgtgacga
     2881 tatgcatgct tcgctcacga tgttctacaa agaaatgggc tgggacccac agcttggttg
     2941 tccgacccgc gaaacattgc agcgtctggg gctggaagat attgccgccg atctggcagc
     3001 acacaatcta ctgcctgtgt aaggaatggg tgagatgaac catcgggatg aattgccgtt
     3061 agcgaaagtc agcgaagttg acgaggcgaa gcggcaatgg ttacagggga tgcgtcaccc
     3121 cgttgatacg gtaaccgaac cagaaccggc tgaaatcctg gcagagttca ttcgccagca
     3181 ttctgcggct gggcagctgg tggcgcgcgc cgtgtttctc tcgccaccct attcggttgc
     3241 agaagaggag ctgtcagttt tgctggaaag cattaagcag aacggagact acgcggatat
     3301 cgcttgtatg acgggaagcc aggatgatta ctattactca acgcaggcca tgagtgaaaa
     3361 ctacgcggcg atgtctttgc aagttgtgga gcaggacatt tgtcgcgcca ttgcacacgc
     3421 cgttcgtttt gagtgccaga catatcctcg tccttacaaa gtcgccatgc tgatgcaagc
     3481 gccatattac ttccaggaag cgcagattga agccgcgatc gccgcgatgg atgttgcacc
     3541 tgaatatgcc gatatacggc aggtggagtc atcgacggcg gtgttgtact tgtttagcga
     3601 gcgatttatg acctatggca aggcttatgg cttatgcgaa tggttcgagg tagagcagtt
     3661 tcagaacccc tgaaatttca gggagatgtc atggataggg tggacagttg cagccactgc
     3721 attaggggat aatcagatgt ccttcactcg acgcaaattt gttctgggga tgggaacagt
     3781 aatctttttt actggttctg cgtcttctct gttagcgaac acgaggcaag aaaaggaagt
     3841 tcggtatgcc atgattcatg acgagtcacg gtgcaatggc tgtaatattt gcgcccgcgc
     3901 atgccgtaaa actaaccacg tccccgctca gggaagccgt ttatcgatag cacacattcc
     3961 agtcaccgat aacgacaacg aaacgcagta tcactttttt cgccagtcat gtcagcactg
     4021 cgaagatgct ccgtgcatcg acgtatgccc gacaggggcg tcgtggcgtg atgagcaggg
     4081 gatcgtgcgg gtagaaaaat cgcagtgcat cggctgcagt tattgcattg gcgcttgtcc
     4141 gtaccaggtt cgctacctta accctgtcac taaagtggca gataaatgtg atttctgcgc
     4201 tgagtcccga ctggcaaaag gctttccgcc catttgcgtc agcgcctgcc cggaacacgc
     4261 attgatcttt ggtcgcgaag atagcccaga aattcaggcg tggttgcagc aaaataaata
     4321 ctatcaatat cagctacctg gtgccgggaa accgcatctg tatcgccggt tcggtcaaca
     4381 tttgattaaa aaggaaaatg tatgaacccg tcgcaacatg ctgaacagtt tcagagccag
     4441 ttagcgaact atgtgccaca gttcactccc gaattttggc cggtgtggtt gatcattgcc
     4501 ggagtattgc tggttgggat gtggttggtg ctggggctgc atgccttgct tcgtgctcgt
     4561 ggcgtgaaga aatcagtcac cgattatggt gagaagattt atctttactg caaagcggtc
     4621 agattatggc actggtcgaa tgcgttactc tttgtattgt tgctggcaag tgggctgata
     4681 aatcactttg cgctggtggg cgcaactgcg gttaaaagtc tggttgcggt gcatgaagtt
     4741 tgcggatttt tgttactcgc atgctggctc ggctttgtgc tgatcaatgc cgttgggggt
     4801 aatggtcacc actatcgcat tcgtcgtcag gggtggctgg agcgagcggc aaaacaaacg
     4861 cgattttatt tgtttggcat tatgcagggg gaagaacatc ctttcccggc aacaacccag
     4921 tctaaattta atcccttaca gcaggtcgcc tatgttggtg ttatgtatgg attgctgccg
     4981 ttgttactat tgacggggct gctgtgtctc tatccgcaag ccgtgggaga tgtgtttcct
     5041 ggcgtaagat actggttatt gcaggcgcat tttgctctgg catttataag cctctttttt
     5101 atcttcggtc atctttatct ttgcaccacg gggcgtacgc cacacgaaac ctttaaaagc
     5161 atggtcgatg gctatcaccg gcactaacga atgatcatta cacgagcaga cctgcgagaa
     5221 tggcgtatcg gagcggtgat gtatcgctgg ttcctgcgcc atttcccacg cggcggtagt
     5281 tatgccgata ttcatcatgc actgattgag gaaggatata cggactgggc ggagagtctg
     5341 gttgagtatg cgtggaaaaa atggctggcg gatgaaaact tcgcgcatca ggaagtttcc
     5401 tctatgcaaa aactggccac agaccccgga gagataccat tttgcagtca gtttgcccgc
     5461 agcgacgatc atgctcgcat cggctgttgt gaggataacg cccgaatcgc aaccgctggg
     5521 tatgccgcgc aaattgccag tatgggttat agcgtgcgga ttggaagtgt cggctttaac
     5581 agtcacattg gcagctccgg tgaacgcgca cgggttgctg taaccggtaa ttcttcgcgg
     5641 ataagcagcg ctggagatag tagtcggatt gcgaataccg gaatgcgagt acgcgtttgt
     5701 acattgggtg aacggtgtca tgtcgccagt aatggtgacc tggtgcaaat cgccagcttt
     5761 ggcgcaaacg ccagaattgc caacagcggc gacaatgtac acatcattgc cagcggtgaa
     5821 aattcaacgg tagtgagcac gggcgtggtc gactcaatta ttctcgggcc agggggaagc
     5881 gccgcactgg catatcatga cggcgaaaga gttcgcttcg ccgtcgccat tgaaggtgaa
     5941 aacaatattc gaaccggagt ccgctatcgt cttaatgaac agcatcagtt tgttgagtgc
     6001 tgatagcttt acctttaatc aaacgaaaga cgccgacgag cacgggatgc aggctttacg
     6061 accgcccgag ccatcgcctc accaatttct gcacatgccg taagtccctg aacgaaaggc
     6121 tggtcgtgca tcaaccaggg caacgcgccg aacgctacgc gtccacgaat atcttcgggt
     6181 tgctgtaacg tataatcttc gccaacatca gggatttcat cccctgtttt ctgtaattgc
     6241 tcgcgtagcc cagggaaagg aatatctttc actttaagcg gacgctgtcc gcgggcatca
     6301 ataaaaacgt caaacgagta gctgttgtct tccgttttca ggacggtgcg cgactcatta
     6361 atttccattt tgtagtcttc accgagggcg agaatatgaa tgattcccgc ttcacgtaag
     6421 gcaagtaggc gacgaataga ctctgaaggg attgccgcat aattatcgat gaaaaccctg
     6481 gcaaggcctt tactgaaccg tttatggtcg tgttcattca gatgaggaac aatttcctgt
     6541 acggcttcat gcaggcgcag aatgacataa cgccagggaa cagtatgttt ttctcgttta
     6601 ttgcgttcga cttcctggag atttttttct gcccagtcaa atgggtcgcg ttgtttgcgc
     6661 tcggcaaacc aggcttgagc aaaggaatcg acattcaggc tctctaaggc tatgcgttgg
     6721 ctccagtctg gatcagcaaa cttgatttcc tctactatca atctaaatac ccgatccaaa
     6781 aggccctctt cacctttttg aatctcagca tttaatgcct gatcggtgac gatgtgtaag
     6841 ggctcgtagg gaatagggca atagaaatcg gcttcgggta aaatacccgt gcgcgacatc
     6901 aacgtaatat ttagcttttc acttgcgtta tcgcggttaa aaacgacgtg ttgtttatca
     6961 tcttcaatga acgaaccatg ctgaatagcc actgccattg ccgcatccag tccgctcaag
     7021 gatgttccca taatacccac gttacacgca tcgacctttg cttccatcag gcctgaccac
     7081 ggactgggaa aatacgttct ggttgcttct tcttcatcag gccagacgtg acccgtggca
     7141 atcaccgcta aatcaaacgt ctcgctgggt aaatcctgat ttgtagcgag catgacgcca
     7201 gcatttgtaa tttgcagatc ggtaacctgg catgattcat aaacagccac tgcaaatttt
     7261 tgctgtcgtg cctggtctac taatcgtaaa aattgatcgc ggaaatattc gcccagcaga
     7321 attcgcggta aaaactgacg atcgtgcaag gtttcttttt taacgctata acgctggaga
     7381 tggctggctt cttgcttttg tagccattcg agatacgtac aattaatcgg cggtatttca
     7441 atactggcaa tatttgccag catcattttt gagttttcct catcactgta tggcatcccg
     7501 acaccggcct cgtcagcctg ctcgaagata gaaatagaaa gtggagtttg ttgctgtaga
     7561 agcgagaata aggtgtagat ccccgtaggc ccggcaccca caatagcaat ttttttcatt
     7621 gttacttacc tgtacgcatt ctttcgaatt tagtgttatc agataagttt ggttgagatt
     7681 tttagaattt gagaaatttc agacagggat gaaaagggcg ctaaccgccc ttgattaaat
     7741 tctgtcaggc gagttttgcc tgattgattt gactcagtgg ctcattgaca tcgaaaactt
     7801 tggcgacaac ttcctcaacg ccgagatttt taagtcggac agtatgtttt tccagatagg
     7861 caagggcgct tttttcatca gtaaaaagat agatcccacc ggcttcgtgg ttcttttcac
     7921 tttctgtcca taccttccac agaaaaccag gttcctgatt aatcgactca gcaagcggtt
     7981 taagctgctc agccattgcg tcgccaaatg ggccattaaa agcaaaatga agttgtaaca
     8041 aagttgccat agcgattctc cttctgataa cgcatacgaa gacagtgtaa atcttttaac
     8101 gtattggata cagagaagaa acagagggaa aaaggagaag accagattaa gaaaatctgg
     8161 tgcgtccgag tggactcgaa ccaccgaccc ccaccatgtc aaggtggtgc tctaaccaac
     8221 tgagctacgg acgcaggatg gtgcgttcaa ttggactcga accaacgacc cccaccatgt
     8281 caaggtggtg ctctaaccaa ctgagctatg aacgcaacgt tgtaggtgac aacggggacg
     8341 aatattagcg gcagagtggg aaggtggcaa gaggcaaaac gtaattttct gcgctatttc
     8401 gaccgtttgc agagctttta agcaaattgg ctatattttg ttgatttgca agggcgattt
     8461 ttattcagga tcgcatttac atctgataca acccaataat tcgtgtatca gagtttaaac
     8521 agagttgttt gccaccatct caaatctgtc gtggataaat cctccgtagc ctttacactt
     8581 aggtagcaat aaaggcacac agatgcagca aaggaataaa tcatgggatc tgatgcgaaa
     8641 aacttgatga gcgacgggaa tgtgcaaatt gttaagaccg gcgaggtcat tggcgcgacg
     8701 caacttactg aaggcgagtt gattgttgaa gctggcggaa gagccgaaaa taccgtggtc
     8761 acgggggctg gctggttgaa agtggcaacc ggtgggatcg ccaaatgcac acagtacggt
     8821 aacaatggca cgctatcggt cagcgacggt gccattgcca cagatattgt tcagtccgag
     8881 ggaggcgcaa ttagtctctc tacgctcgct acggttaatg gccgccatcc cgaaggtgaa
     8941 ttcagcgttg atcagggtta tgcctgcggt ttgttgctgg aaaatggcgg taacctgcgt
     9001 gtactggaag gacatcgcgc ggaaaaaatc attctcgatc aagagggcgg cctgttggtt
     9061 aatgggacaa cctcagcggt cgtggtagat gaaggtggtg aattgttggt gtatccaggt
     9121 ggggaagcca gcaattgtga gattaatcag ggcggcgttt ttatgctggc cgggaaagcc
     9181 agtgatacgt tgcttgctgg tggcaccatg aataatctcg gtggtgaaga ctctgacact
     9241 attgttgaga atggatccat ttatcgtctg gggacggatg gccttcagct ctacagttcc
     9301 ggtaagacgc aaaacctgtc cgtgaatgtg ggtggtcggg ctgaagtgca tgccggtacg
     9361 ctggaaaatg cggtaataca aggtggaaca gtgatcctgt tgtcacccac cagcgcggac
     9421 gaaaattttg tcgtagagga agatcgcgca ccggttgaac tgaccgggag tgttgcatta
     9481 ctggacggcg cttcaatgat tattggctat ggcgcagatc tgcaacaatc aacgattact
     9541 gtacagcagg gcggtgtgtt gattctcgac ggcagtacgg taaaaggtga cggtgtcact
     9601 tttattgttg gtaacatcaa tctgaatggc ggaaaactgt ggctgatcac tggtgcggca
     9661 acgcatgtgc aactgaaagt gaaacgcctg cgcggagagg gagcgatttg cctgcaaacc
     9721 agtgcgaaag aaatctcacc tgacttcatc aatgtgaaag gggaagttac cggggatata
     9781 cacgttgaga taacagatgc cagtcggcaa actctgtgca acgctctgaa attacagcca
     9841 gacgaagacg ggattggcgc aacgctccag cctgcgtaaa aacacaggcg caacccgtga
     9901 aggttgcgcc ttgtctttat tagcgggatg ctcgttgcag aatgataact gacggcagac
     9961 gttgcaggaa ccgcatacgc agcatcatca taatggctgc cgacgtcagg ccaataataa
    10021 agcctatcca gaagcctgct ggccccatag gttcaacgac cagatcggtc agtgccagaa
    10081 tatagccgct tggcaagccc agcacccagt aagccgtaaa ggtgatatag aaaatggaac
    10141 gcgtatcttt ataaccacgc aaaatcccac tgccaatcac ctggattgag tcagaaatct
    10201 gatataccgc cgccagcaac atcaaatgcg cagccagcgt tacaacctcg ggattgtcgt
    10261 tgtacaacag ggcgatttgc tcccgcagtg aaaccgtgaa tatggccgtc agggttgcca
    10321 tacagacacc caccataagc ccggtccgcg cagcggtttg cgcatccagc gttgagccct
    10381 gacccagacg ataacctacg cggatagtta ccgctgccgc cagcgacatt ggaagcacaa
    10441 acatcagtga actaaagttc agggcaatct ggtgtcctgc gacatcaaca ataccgagcg
    10501 gagacactaa cagagccacg acggcaaaca gtgtcacttc aaagaatagt gccagcgcaa
    10561 tcggcaaacc gagttgaatc agtcgtttca taaccgcagg atcgggtttc gcggtgcctt
    10621 tttcgttacg aatatcgcgc atggagcggg cgcgtttaat gtaagaaacc atggcaagga
    10681 acatgaccca atacaccgcc gcagtagcca cgccacaacc aacgccaccg agctcaggca
    10741 taccgaaatg accataaata aagatatagt tcaccggaat gttcaccagc aggccgataa
    10801 agcccattac catacccggc ttggtttttg ccagaccttc acattggtta cgggcaacct
    10861 ggaagaacag atagcccggc gcgccccaca acaacgcacg cagataaccc acggctttgt
    10921 ccgccagagc cggatcgatg ttttccatgg agcggataat gtaacctgca ttccacagca
    10981 caagcataat gagaacggaa acaaaacctg ccagccagaa accttgtcgc acctgatgcg
    11041 caatgcgctc acgtcgaccg gaaccattta attgcgcgat aaccggcgtt aatgccagca
    11101 gcagtccgtg accaaagagg atcgccggaa gccagataga agtaccgata gcgaccgccg
    11161 ccatgtcggt ggcactatag ccgcccgcca tcacggtatc gacaaaaccc atcgcagttt
    11221 gggcgatttg cgcgagaatc accgggattg ctaatgctaa taacagacgc gcttcactga
    11281 tatacttctg cacgtgaaca ccttttattt gtagttatat gagagactaa aaagccgcct
    11341 gaatgggcag caaaaagaag aagcagggga aattcagtct attgtagcga ggatttatta
    11401 attctccagt gaaaaaatag tcattcttac cccttcactg gtaagggcgt ttgccacctg
    11461 ctattgtgct gaacagaatg tcttaactga tttcaggagt tgtaagtatg tttacgggga
    11521 ttgtacaggg caccgcaaaa ctggtgtcga ttgacgagaa accaaatttt cgtacgcatg
    11581 tggtggagtt acccgaccac atgctggacg gcctggaaac aggtgcttcc gtggcgcata
    11641 acggttgctg cctgaccgtg acggaaatta acggcaacca tgtcagtttt gacctgatga
    11701 aagaaacgtt acgcattacc aatcttggcg atttaaaagt gggggattgg gtaaacgttg
    11761 agcgtgcggc gaaattcagt gatgaaattg gcggacactt aatgtcaggt catattatga
    11821 ccaccgctga agtggcgaaa atattaacct cggaaaataa tcgccagatc tggtttaaag
    11881 tccaggatag tcagttgatg aaatatatcc tgtacaaagg atttattggc atcgacggta
    11941 ttagcctgac cgtcggcgaa gtcacgccaa cgcgtttttg cgtccattta attccggaaa
    12001 ctctggaacg cacgactctt gggaagaaaa aactcggcgc acgcgtcaac attgaaattg
    12061 atccacaaac tcaggcagtg gtagatacgg tagaacgtgt gctggcggca cgagaaaatg
    12121 ccatgaatca accaggtaca gaagcctgat ctgaaatcgc ccccggagag ggggcgatag
    12181 aatagccttt agcgagcgac tcgaaggccg ttttcaacac cgcgtgagaa cacgacctgc
    12241 cagagctgaa tatcacgggc gcggaaagca cctgcacagg cattcagata ataggtaaac
    12301 attcgtttaa agcgttcact gtagttatcc gcaatttctg gccatgcggc gaggaatcgt
    12361 tcataccacg ccatcaacgt agtatcgtaa tcagcaccga agttatgcca gtcttccatc
    12421 acgaagtggg gttcgctgga ttgagcaatc tggcgtacag agggcaggca accgttcgga
    12481 aaaatatatt tattaatcca gggatcaaca ttcagatcgg tttttttcga accgatagta
    12541 tggagcagga atataccttc cggtttcaaa ttacgatcca ccaccgcaaa ataggtatcg
    12601 taatttttcg gtccgacgtg ctcgaacatc cccacagaaa caatacgatc aaactggtcg
    12661 ttcaggtcac gataatcttg cagcaaaatg gtgacatcca ggccttcaca gcgttcctga
    12721 gccatttttt gctgttcggc agaaatggtg acgccgacca cgcttacgtc atagttagat
    12781 gccatgtagt gcgccagtcc gccccagccg cagccaatat ccagtacgcg catccctggt
    12841 tttaactgca atttttcaca aatcattttg agcttcgcct gctgggcaga ttccagatta
    12901 tcggcatctt tccagtaagc gcaggaatat tgcatgaagg gatcaagcat gcggctgaac
    12961 aagtcattac ccaaatcgta atgctccttg ccgactatcc aggcacgttt tttactctgc
    13021 agattgaaga gacgagcgct ggcaatacgc agcgtgtctt tgaaatgatg ggggagttgg
    13081 ttctcgagac ctgcgcgtaa gactttgcta aaaaacatat ccagtcggtc acattcccac
    13141 cagccatcca tataactttc gcctaacccc aaagagcctt cttgcagaac gcgtttaaaa
    13201 aaatcggggt ttttcacacg aatatccgcc ggggcagaac cgttaatggc tataccggca
    13261 cggctaagta attcgttggc gatacggtac cagttgtcat ccggtacact gacttcttct
    13321 atacacgatg aactcatagt ttctccatca ctggtcggtg atcagaacct taaaacagcg
    13381 tagacgcttt ttgggctttg tgagaaatct cgcagaaaaa accgcgagtc tataatccga
    13441 cgtagaacag agggaaagga ggaacctttg ccgaaatgac catccatagt gagtcgggaa
    13501 cgatcctgtc cccgtaaaaa cataatattt atcgtagctt taacaactcg aaattagtat
    13561 aggccgctaa tttcaatcat acaacaaaat tttgttagca gcctaagtat aagtatatcg
    13621 atatagatca gtgtgattcg ctatgagcga cttcggcatt gccatgattc tggcagccaa
    13681 cttcttcaca acgttgcatc atgtaaccca gagcgaccag cactactgtt gataacatca
    13741 cgctggtggt ggtgagcaat ggcgtgctga tactgatcag ccaggaaact accagacttg
    13801 cgaggaagca cagacccagt tgtagagtgt tctgcaacgc tgcggcgcga ccagttgcgt
    13861 gtgggaaggg acgcagcgcc tgggcgacaa caatcgggta gatcgcgcca ttggcaatcg
    13921 ccatcacaca gaatgggatc aggatttcga ccagcgacac atggctaatg aagcctgcag
    13981 cccaggtcgc aatgacgctg actgcaaaca gcaccagcaa ccacggtaat aactgcttgc
    14041 cttgccattt ctgcagcgcg gcgcgacagc cataaccacc aatcagaaac gcgatagttt
    14101 gcgggacata acttaaacca ataactgccg ggctgtagcc catttcacta aggatgaacg
    14161 gtgaaccggt cagccatgca aaaaaactgg ctgaacaggc cgcgtatatc agcacgttgc
    14221 cgcgataggt tttagaacgt agcaggtcgg taaaggtcag accatcctga ctattgttac
    14281 gggccttcgt cgtgggtttg agccagaaaa taggcagaat cagcaccacg gtaatggcaa
    14341 acagggtggc gaaaatcgcc tgccaggaaa aatggaccag cagccagctt cctaacagag
    14401 gagccagggc cggagataga cccaccagcg gcatgatggt cgcaaaaatt cggttaactt
    14461 tctgtgaagg ataataatcc gtcactaacg cttgccagat aaccgccgcg gcgcagacac
    14521 ccacagcctg tacaaaacgc aataccagca gcgtagcggc gttttctacc cacagcatcc
    14581 ccagactacc taacgcaaaa attgtcaggc cgattaataa caccggttta cgaccataac
    14641 ggtcggagag tggcccccac agaagctggg ctgcggcaaa accggcaagg aacagactaa
    14701 ggctggcact gacagcagac gcaggcgttt gcaggtcggc ctgtatggcg gcgaaagcag
    14761 gcagatacat atcggttgcc agaaaaccga gtacgctcaa acccgccagc cagactaaaa
    14821 atcttttccc aggttgcatg tatattctct aaatctctca attaaacacc gccgcaaagt
    14881 gtagggagtg aaattagtgt tgtgaaacgg taatatttga tgactggttt caaaaatttt
    14941 gcaggcagac tatgtggtca gaatattcac tcgaagttgt tgatgcggta gcgcgtaatg
    15001 gtagttttag cgctgcggca caggagctgc atcgcgttcc ttctgcggtc agctataccg
    15061 tgcgtcagct ggaagagtgg ctggcggtgc cgctctttga acgacggcac cgtgatgtgg
    15121 agctgaccgc tgctggagcg tggtttctca aagaagggcg ctctgttgtc aaaaaaatgc
    15181 agatcacccg ccagcaatgt cagcagatag cgaacggctg gcgcggtcag ttagctatcg
    15241 cagtggataa tattgtcagg ccagaacgta cacggcagat gatcgttgat ttttatcgcc
    15301 attttgatga tgtcgaactt cttgtctttc aggaagtgtt caacggtgtc tgggatgcgc
    15361 tttccgacgg gcgcgtggaa ctggcgattg gcgcaacacg cgcgattccg gtaggcggtc
    15421 gttatgcctt ccgggatatg gggatgctaa gctggagttg cgttgttgcc agccaccacc
    15481 cgctggcgtt gatggatggc ccattcagcg atgatacgtt gcgcaactgg ccgtcgttgg
    15541 tgcgcgaaga cacctcgcga acgctaccta aacgtattac ctggttgctg gataaccaaa
    15601 aaagagttgt cgtgcctgac tgggaatcat cggcaacctg tatctcggca ggattatgca
    15661 tagggatggt gccaacacat ttcgccaaac cgtggcttaa tgaggggaag tgggtagcac
    15721 ttgaattgga aaaccccttc ccggattcgg catgttgtct gacatggcag caaaatgata
    15781 tgtcgcctgc gttaacctgg ctgcttgagt atctcggcga tagtgagacg ctgaataagg
    15841 agtggctgcg ggagccggaa gagactcccg caacgggtga ttaacgacga tagtcgcgga
    15901 acgggccgtc agccacggag cggcgttcaa tcaagcgcgg atgcacttca atggactgcg
    15961 gttcttcacg tttgttgacg atacgatcca acagcatgtt gaacgctgtt tcacccagcg
    16021 aatcttttgg ctggtggatc gtggtcagcg ccggcgtaaa atagcgggcg ttgcgcacgt
    16081 tatcataacc gatcagcgaa acatcctgcg ggacgcgcag acccatttca tcagcagcac
    16141 aaagtgcgcc cattgccatg atatcgccac cacagaagac ggcagtaggg cgatgcggct
    16201 gcgacagtat ttgctgcatg gcgcgataac cagattcggg ttcaaagtca ccctgaacaa
    16261 tccagctttc cggcaccttg atcatcgctt cttccatcgc cttcataaaa ccggcaaggc
    16321 ggcctgcgcc ggtgttacgt tccagcgggc cggggatgac gccgatttcg cggtgaccgc
    16381 gttcaatcag ataacgcccg gccatgtagc cgccttcgaa cgcgttatca atgaccgcat
    16441 cggtgaagtc agcttttgct tcaccccagt ccatcaccac cattgggata tggcgatact
    16501 cttccagcat cgccagcaac ggctctgggt actcagaaca catcaccagc agaccatcga
    16561 cgcgtttttg cgccatcatc gacagataag cccgctgttt ctcaagattg ttccacgcat
    16621 tacccagaat cagggtgtaa cctttctgga agcaattttt ttcaaccgct tcaatgatct
    16681 cggcaaaata ggccgcttcg ctgctggtcg ccagcaaacc gatagacttg gtgtggttaa
    16741 ccttcaggct acgcgccacc gcgctagggg agtagtgtaa ttctttaatc gctgcccaca
    16801 cggcgttgcg cgtttcttca gcgacgaaac gtgttttgtt gattacgtgt gacacagttg
    16861 tagtggaaac gtttgctcgt ttcgctacat cttttattgt tgccattcca tttcactcca
    16921 gaccctaact tcagctcctg caaaatcgca aggtaaacgt ttgcctttac acaccttacg
    16981 cacaacaaaa gtgttgcggt acgccggaaa aacgacacgg agcgtcagga aggggtcaat
    17041 ggccagtacg caaatagtgt aagcgtggaa ttttggccga tcttgacgaa aaggggaagt
    17101 ggtaaaaaca cttatcatta taaatgacgg gagatttttt catcacagtg tgtaaaaatg
    17161 cgtaatatca ctatttgtgt ggtaataaaa aaggagaaaa cttgatgagc accgacctta
    17221 aattttcact ggtaacaacg attatcgtcc tcggtttgat cgtggccgtg ggtttgactg
    17281 ccgcgctgca ctgatttcca ctcgagggag cgcgttctcc ctcgcatctc cttcctgttt
    17341 gttagcaaaa tggcaaaagt gttttctctt tttgttattt tcattttttt gtgattgctg
    17401 tcatgctttc gccactttaa tgaatatcgc gtaatgacgc gaagaggtgg agataaggca
    17461 tgaaaattaa ctatccgttg ctggcgctgg cgattggcgc gtttggtatc gggacaacgg
    17521 agttctcgcc aatgggcttg ttgcccgtca ttgcgcgcgg tgtggatgtc tcgattcccg
    17581 ctgccggaat gttaatcagt gcctatgcag ttggcgtaat ggttggcgcg ccgctgatga
    17641 cgcttctact ttctcatcgt gcccgccgca gtgcgttgat tttcctgatg gcaattttca
    17701 cgctcggcaa cgtactttcc gccatcgcgc cggattatat gaccctgatg ctttcacgca
    17761 ttttgaccag cctgaatcac ggagcatttt ttggtttggg ttcagtcgtg gccgcaagcg
    17821 tggtgccaaa acataaacag gccagcgcag ttgccactat gtttatgggg ttaaccctgg
    17881 caaatatcgg tggcgtgccg gcggcgacct ggttgggtga aaccatcggc tggcggatgt
    17941 catttctggc aacggcgggg ctgggagtga tttcaatggt aagtctgttc ttctcattac
    18001 ctaaaggtgg tgcaggggca cgacctgaag tgaaaaaaga gctggcggta ttaatgcgtc
    18061 cgcaggtgct gtctgcattg ctgacgacgg tactgggagc tggtgcaatg tttactctct
    18121 acacctatat ctctccggta ctacaaagta ttacccacgc aacaccagtg ttcgtcacgg
    18181 caatgctggt gctgattggt gtcggattct ctatcggtaa ctatctcggc ggcaaactgg
    18241 cagatcgttc agttaacggc acgttgaaag gctttttgtt gctgctgatg gtgattatgc
    18301 tggcaatccc gttcctggcc cgcaatgagt tcggcgcagc tattagcatg gtggtgtggg
    18361 gcgcagcaac ctttgcggtc gtaccgccgt tacagatgcg cgtgatgcgt gtcgccagtg
    18421 aagcgccggg tctgtcttca tcagtcaata ttggtgcctt taatcttgga aatgcgctgg
    18481 gagcagctgc tggtggtgcg gtaatttccg ctgggctggg atacagcttt gtgccggtga
    18541 tgggagcgat tgtcgcggga ctggcattat tgctggtgtt tatgtcagcc agaaaacaac
    18601 ctgaaacagt ttgcgttgct aacagctaat taaacaaaaa cgcagaagga taagcttctg
    18661 cgttacagtt gctgacaacg tgcgatttat cttgccgaat gcgctgttaa cgcctcatcc
    18721 gcgttggcat ggtaagcgaa gcgcatcagg caatgctgca tttgccatca gttattatgc
    18781 agcgagattt ttcgctacga attcccagtt caccagcgcc cagaagtgct ccagatagcc
    18841 aggacgtgca ttgcgatagt cgatgtaata agcgtgttcc cagacatcaa cggtcagcag
    18901 cggagtcgca tcggtggtca gcggagtacc cgcgttagag gttgaaacga tagccagttt
    18961 gccatcgctg tttttcacca gccaggtcca gccagaacca aagtttttga tcgctgcatc
    19021 agtaaactgc gctttgaaat cggcaaagct gccaaaagat gcggcgatag cttcagcgac
    19081 ttttccagtc ggttcgccac cggcgttcgg tgccaggcag ttccagtaga aagtatggtt
    19141 ccagacctga gctgcgttgt tgaatacgcc accttcagag ctgcgaataa tctcttccag
    19201 tgatttacct tcaaacgcgg tacctttaat caggttgttc aggttagtga cataagtctg
    19261 atgatgcttg ccgtagtgat actcgatagt ttccgcagaa atgtgcggtg ccagagcatc
    19321 tttagcatat ggtagtgcag gtaattcgaa tgacattgct actctccttt attattaatt
    19381 tgcatacgta caatagcctt attgtgcgta tggatagggt agcaaattaa aaggtgagac
    19441 aaaagatgaa cttaccctgt tgccgtaaca acagggtaaa gttttagcga agtgtttttg
    19501 gggtcattac ccgacgagcg ccaacatagt ggcgctgcca gtagtcttca ctgagagaag
    19561 tgatttgaat ttcctgacct gtgcgcggtg actgaataaa tttgccgttg ccgacataca
    19621 cgccgacatg atcggctgtg ccgcgtccct gagtacggaa aaagaccagg tcgccgtttt
    19681 tcagttcact acgttcgatt ggcgctgcat cacgcaggtg atacatttca ttcgccgtac
    19741 gcggaatacg aattttcacc aaatctttat aagcgtaata aaccaggccg ctgcaatcaa
    19801 aaccggtacg cggtgagctg ccaccccaac gatatggctt accaatttgc tgcatcagtt
    19861 tattcattgc cactttcgta gctttttgca ccttcgcttt gtgcgcgtcg gcaagagttc
    19921 ctgacgcggc atttttgact ttcacacaat gcgatttata gcccttacgt ttggtacact
    19981 tttcggtaaa gctggctgag gccgttttat tgacggcgtg tttgctgcga gttttggcgt
    20041 ttttagaagc ggtaatggaa gattttttaa cggaagaagc ggttttgctt ttggtcgtac
    20101 ttttactggc ggcttttttc gctgttttct gggtcttttt ggtggttgcc gtgcttttct
    20161 ttttatctgc ttttttggtg atatgagtgg tagcagaact ctccctggct tgctttgaag
    20221 catgggccat aggcgttaaa ggcagggtgg taaaaagtaa agcacagagc gtgatcgaaa
    20281 tacggtttat ccgcgccact gagcgtccct ctgttaaggc aggtctatca tgcctgcgtc
    20341 tgatttacaa aacttgatga ttctaatgca tagaagaaga gaaagttaat agcttttatt
    20401 gttaaaaaat acgtttcgtt ggcgagcgca aaaaaatgca gcagtgaaag ctaaaaatgg
    20461 ttataaatac gccagtttgc cgcgcatcag agaagaaacg aatatttgaa atgcaacaaa
    20521 tagagcaaag ggataaaata gcaaaagcgt gatgaaaatg ttattttccg atgccgcact
    20581 gacccgctac aatgtcagat tactgccgta aagaagtttg aggaagcaag acaatgagca
    20641 ccactatcga aaaaatccaa cgccagattg ctgaaaaccc gatcctgctg tacatgaaag
    20701 gttcaccgaa actgccgagc tgcggtttct ctgcccaggc agtccaggcg cttgccgcat
    20761 gtggcgaacg ttttgcctat gttgatattc tgcagaatcc ggacattcgt gcggaactgc
    20821 cgaaatatgc taactggccg accttcccgc aactgtgggt tgacggcgag ctggtcggcg
    20881 gttgtgatat cgtgatcgaa atgtatcagc gtggcgaact gcagcagctg atcaaagaaa
    20941 ctgccgctaa atacaagtct gaagagccgg acgcggaata atccgtcctg caatactgac
    21001 ggatgcgaac gggcatccgt cagtcctttc tctatcccca atccagccct tgtggcgaac
    21061 ttgaaaatcc cgcctcacgc agcagcgtaa acatcggcgt ttgccggacc ggtagatcat
    21121 tcacttctgt tagcgtaaag cgcagccgtg gttcgcgacg cagtgcggta gtcagcgcgt
    21181 ggaaaacctc tggggcgagt aattcctctt tttcctgcca gaccagcatt tttttgccac
    21241 cttgcgccag atagagtaac aatttgccgc cagaaacgac caccagcgca cccgcccgac
    21301 gcgttggaac cagcgttgcc ggatgtgcag gccagggcag aagatttccc cacacattag
    21361 ccggatcgtt ggcagagagc gccactggtg tatagtggcg cgtttgcgtg gcttgtgtcg
    21421 ccagatcgcg caatcggtca atagtcagac gttcagcgaa ttgcgcgcca cccagacctt
    21481 ctacaaaacg acctcgcata attcgcccgg agtcttccat acttcgacaa agcgtttgca
    21541 tcgatggaaa cccgccaggg atattttcgg ctatcaccgc ctgacgactg atgatgccgt
    21601 agcggtcgag catattttcc gccagcgcca gcatcctttc ggtatcgttt agtggctcca
    21661 cctgcaataa cgaccagcgt ccagccagat ttggtgtgtt gtaagatacc agcggcgaga
    21721 cgggttgcgc atagacagga cgtccacgac gagcccggtg actgcggcga gttgaggtgc
    21781 gtgcgttgga actgctgcgg gtgagggcgc gtaacggtgc ccaaatgtcg ctggtgatga
    21841 cgccttgcca gaccagcgcc cataacgcct cttgcagggc agagagatca accgattcgc
    21901 cgattttgtc gcgtatccgt tggctgattt gttgtgcaaa ccaggctcct ccgtcagcca
    21961 gaacagcgac tatcgcttgt tgcagcgccg aacgattcgc ctgatccgct tcggcgggag
    22021 tgaacgattc tgcagcatat tcctgtagat gcagtgccac caggccgtca tcttcaccca
    22081 gttttttttg ccccgaccag ataaccgcac cggttgccag taattcatcg agcatttctg
    22141 acgaatagtc gcgtacgcgg gcaggcagga tctggctttc ccagagtgag gcgggtaaac
    22201 cgactccggc aagctgttcg atcacccgca tcacgccatc tacgccctca taaacgcctg
    22261 gcgatgttga ggcaaagagc gccgggctac catcggtggc gggtaatacg ccctgacgtt
    22321 ccagcagcaa tcgcgcatag gtcgtggctg caacgggacg cgtcgcttct ctggcggctt
    22381 gcagcgagcg caaacgcaga cgacgaaata cttcatcgct cacccagatg tcttgttgca
    22441 gattcatcac cagaccctgt tcacgcagtt gctgaagctg ctcttcgaca atggcaatac
    22501 cgagactaaa ctcatgagcc agttgttcag ccgtgaccag agcatgagcc cggagatagc
    22561 gcagaaagag atcgcgaagc gggtaactga ctctatgtaa ataaatctct ggcaatgact
    22621 ctggtagtcg tacgccgagg gcatcacgca gcctggcggc atcatccata caggcaagac
    22681 gctcctgtcc gctaatcatc gctgggaaga ttcgttttac tgcaagaaga ttttccagat
    22741 acgacgcaac ctcttcactg ctgcctgtat gccgttgcgc caggtcttca acggtcattg
    22801 gccccagttc gcgcagcagg tcgaacaatc cttcttcacc tttcgctctt ctgccaggag
    22861 ccagtcgttg caactcttct tccacctggc gaatgacctg cgggtcgagt aattcccccg
    22921 gatcgacctg tccgagtaga ttgcgcagta actcgctgtc cagcgacagt acggatgccc
    22981 ggcgctctgc cagcggggcg tcgctctggt acataaattc cgcgacatag ccgaacaata
    23041 aacttgtggc gaaaggcgag ggcgtagtgg tcgttacatc ggatatttga atttcgccac
    23101 cgttcaggcg acgcatcaaa cgttccagtg cgggaagatc ataaacatct tgcagacatt
    23161 cgcgtagggt ttcgagaatg accggaaaat ccggatatcc ctgagcgatt tccagcaact
    23221 gactggcgcg cagccgttgt tgccaaagcg gggtgcgatg gcccggagtg cgccccggca
    23281 ttaataatgc ccgcgcggcg cattcgcgaa aacgggcggc gaaaagtgcc gagctgccta
    23341 ccgcctcgcg gacaatttgc agcaactttt ctggttcaaa caaaaaaatc gcggcgtcgg
    23401 gcaatttacc atcagtgtca ggaatgcgcg caacaatgcc gtcatcactg gcgaccacag
    23461 acgcgtcagc gccccatagg gcatgtattc gcccggcaat cgccagcgcc cagggttcat
    23521 gcacccgtct tccataggga gagtgcaaaa taatacgcca gtcgccaatt tcatcacggc
    23581 accgttcgag gaccagatgg cgactccccg gaacgatgcc cgtcgcgttg cgttgctcgt
    23641 caatcaaccc ctgaatattg gcgttcgtat tttcttctgc cagccacggg ggaatagtcc
    23701 cggaaaagaa cgcgccatcc gccagtaaat gaagaaaatc gccgatcatc tcgcctaatt
    23761 cagccggacg tccgttacct tcaccacgcc agaaggggag ccgggcagaa cgacccggag
    23821 caggagtcac aatcacctga tcgcgggtga tttgctggat ccgccatgag gtagcgccga
    23881 gagtgataat gtcgttcacc cgcgactcat ataccatctc ctcatccagt tcacccaccc
    23941 gccgcgaacc ggcctgttct tcaccttcgg gtaataacac gctatacatg ccacgatccg
    24001 gaatggtgcc gccgctggta accgccaaca attgagcgcc aggtcgggcg gtcaatatcc
    24061 cggtctccct gttccagacc agtttggggc gaaaagcaga aaaatcgcca gagggatagc
    24121 gcccggaaag catatccagc gtggcgtcaa aaacacgtct tggcagatct ttccacggtg
    24181 cggcacggcg tacgcgggag taccattcgt ctacctgtaa tgcatccatc gccgcggcgg
    24241 caacggtttg ctgcgcaagg acgtcgagtg gattatgcgg cggtgtcagg ttttccagcc
    24301 tgcctgcgaa catacactct acaatgactg cggaatcgac taagtcacgc cgggtacgag
    24361 ggaaaaacag ccctttagat acaccgccaa cctgatgtcc ggcgcgacca atgcgttgta
    24421 acccactggc aacagaaagc ggcgttgcca cctgaatcac cagatccacc gcgcccatat
    24481 caatccccag ttcgagactg gaggttgcga ccacgcagcg taattcaccc gatttcagcg
    24541 cctgttcggt gattgctcgt tgttctttag agacggagcc gtggtgtgag cgggcaataa
    24601 aaacgtcgct actttgtaca tggttagagg ttgcgccgga ggtcgactcg aaatgggccg
    24661 tatcaacggc gatagacggg gaacgctgta agcgtgcggc gtaaagctca tttaatcgtg
    24721 ccgtcagttt ttccgccagc ccacgtgaat tagtaaagac aatggtcgag cgatggcgca
    24781 acacttcatc aaggataccc gtttcaatat atggccagat ggagccttcc cggccggcat
    24841 ggctgtcttc gccggtgccg ctggcgaccg atgagacatc atccatattg gcgaccggta
    24901 cgacaattcg tatctgcgga tggcgcattg cgggcgggtt gactaccgta accgggcgat
    24961 cgccaccaag aaatgctgcc acatcgctgg ctgagcgcac agtggcagaa aggccaattc
    25021 gctgtgctga ggtgtggagc agcgcatcga gccgctccag acttaacgcc agatgcgcac
    25081 cacgtttact gcccgctacc gcgtggactt catcaataat taccgtttcg acgccgcgta
    25141 gcgtttcgcg cgcgcgggag gtcagcatca gatagagtga ttcgggtgtg gtgatcagaa
    25201 tatccggcgg attacgggtg agtttgctgc gttcctgtgc aggcgtatcg ccagtacgga
    25261 tccctacgcg aagattgact tccgtttcgc cgcgccgccg ccgttcatcg gcaataccct
    25321 tcaacgggag ctgcaagttg cgctgaacgt cggtgcccag agcttttatc ggtgaaatat
    25381 agaggatgcg tgaggtttta cgcttatgcg cctcgcgggt atcttcgccg ccttcgcgga
    25441 agagccgatc gagggcgtag agaaatgctg ccagcgtttt cccggagccg gtcggtgcaa
    25501 tcaccagcgc atgttcgctt cgcgccgcca catgccaggt ttgcgactgg acagcggtcg
    25561 gctgtttaaa ggcgcgaaga aaccagtcgc gggtcgccgg tgaaaacacg tccggcagga
    25621 gcgatgaagg gtctggatta tctgccatta acgctattcc ccgtgaatat catgcttgat
    25681 gcgtggaata gcctcactat agatgcctga atcattgcat gtcatcaggc atcgactcga
    25741 ttacacctct tcggcggcag gtagcggcca gcctcccaga cgtttccagc ggttgacgat
    25801 ttcacaaaac agcacagcag tgcgttcggt gtcgtacagc gcggagtgcg cctgggtgct
    25861 gtcgaagtcc atgccagcgg tctggcaagc ctttgacaat acggtttgtc cgagtgccag
    25921 cccggccagt gcagcagtgt caaaagtggc gaaagggtgg aacgggttac gtttcagtga
    25981 ggcgcgttct gcggcggcca tcataaagct gtgatcaaaa ttggcattgt gcgccaccat
    26041 aatggcgcgg ttacagccgc tcgctttaat acctttacgt acaactttaa aaatttcgtg
    26101 cagcgcctcg tattcgctga ccgcgccgcg atcgggatcg ttcgggtcaa tgccgttgaa
    26161 ggcgagggcc tctggctgca aatttgcgcc gacaaatggt tcgacgtgga aatgtaatgt
    26221 ggtgtccggc atcagccagc cttgttcatc cattttcagg gtgatggcgg caatctcaag
    26281 cagcgcatcg gttttggcgt taaatccggc tgtttcaaca tcgatcacaa caggataaaa
    26341 accacgaaaa cggtcgcaca gaccggtaag ttgagcgtta tcggacatct gattctctta
    26401 atacggataa attgcagcgc gcattatgac aaatattact tgtcgatgca gtaaagatgc
    26461 gggcgcgatg agttcacgcc cggcaggaga ttagttgccc agaccgtgac cggcgtcttt
    26521 ctcttcgatt aactcaattt tgtaaccgtc cggatcttcc acaaacgcga taaccgtagt
    26581 accgcctttt accggacccg cttcacgggt cacgttaccc ccgttttgac ggattttttc
    26641 gcacgcttca gcggcgttat ctacgctaag cgcgatgtga ccataagcag tgccgagttc
    26701 gtatttatcc acgccccagt tgtaggtcag ttcaatcacc gcttcttcgg tttccgggcc
    26761 gtagccaaca aacgccagtg agtatttgta ttccgggttt tcgctggtac gcagcagttt
    26821 catgcccagc actttggtat aaaaatcgat ggagcgttgc agatcgccaa cgcgcagcat
    26881 ggtatgaaga agacgcatct ttttatcctc aaaatggcta acagattcaa aatgttgttt
    26941 tagtatagcg gcggctttac gccgctcgca atgttggatt acaacgtcgg gtaatcggta
    27001 tagccttccg cgccgccacc gtagaaactt tcggcacgct gtgggttaag ctcagctttg
    27061 cgctgcaagc gggcgaccag atccgggttc gcaatccagt cacgaccaaa tgccaccgca
    27121 tcaattaacc ctttgccgat cagcgtttca gctttttcta ctgtgtatgc acctgcgccg
    27181 ataatcggac cgtggaaacg ggcgcgtact ttttcgcgga acgcatcagt atacggttca
    27241 ccccccgccc aatctggttc tgacatatgc agataagcaa tgccgcgttt acccagttgt
    27301 tcaatcagat acagtgcatc ggcttcttca ttcgggccgt tatccgtgtt ctggaaagta
    27361 ccgattggcg aaacgcgaat gccaatgcga tcggcacccc attcttcaat cccggcatcg
    27421 accacttcca gtaccagacg tgcgcgattt tccacgctgc cgccgtactg atcggtacga
    27481 tggtttgaag aaggagaaag gaactgatgc agcaaataac cgtgagcaga gtggagctct
    27541 accagatcaa aaccggcttc acgcgcgtta gcaatggcct gacggaaatc attgacgata
    27601 cctggaatct cttccagttc aagcgcacgc ggcatggatg tttcaacacg gatcgcctga
    27661 ccattttcat cgcgcagaga agtacgtgtt cccgcgctaa gtgctgaagg cgctaccggt
    27721 gcctgaccgc caggttgcag gctggcgtga gaaatgcgtc cggtgtgcca cagctgcacg
    27781 gccatatgac cattttcagc atgaacgcca gcggtgattt ttttccatgc ggcaatttgc
    27841 tccggactat ggatgccagg cgcacctgca tatccttttg cctgggcaga aatttgcgtg
    27901 gcttcactaa taatcaaacc ggcactggca cgttggcgat agtattccgc catcaacggg
    27961 gtaggaatgt cacccggttc aatactgcgc agacgcgtca gcggtgccat aaaaatacgg
    28021 tttgccgccg tgatcgcgcc cactttcagt ggggaataca gtttttcaga tgacataaag
    28081 gctcctggtc gtctggtgat aaaaaatgcc tgctaaacgg caggcgtcgc aataatgttt
    28141 tttacatggg ccagcgcgtt ttccagtggc tcgaaactgc gcgaaatttt ggcctgcaga
    28201 ttcgcgccaa gccacagtgc gtaaagcact tgtgcctgtt gtagcggttc gccacaaaag
    28261 gttaaacaat ggttctcacg gccattttcc agcgcctgag agagcagggc gatcacgccg
    28321 cgagcacctt tatccatcgc gctgcgcata tcttctgaca gatcgcacac ttcggcagag
    28381 agttttactg tcaggcaacc actgatggtt ccatgttggc aaaactggtt cagggtttgc
    28441 tggtaataag ccagtatgcg gtcgcggtag ttaccttcgc cggattgcag caactcagtc
    28501 agtcgctggt gatatgcggc gtaatgacgc tcaagcatgg caacgccaaa cgcttcttta
    28561 gagcgaaagt agtgatagaa ggaccctttc ggcacttcag cggtttttag taattcgctt
    28621 agccccatcc cggtgaatcc acgttgcagg caaagttgct cgcccgtcgc caggagatgt
    28681 tcgcgagtat catgttcggt gtgtttgttc atgcgttgga gtgtagtaga ccagtcggtc
    28741 tagtgcaagc aaagttgcta ctttggacat taacagcttc aataatggta ataatcttat
    28801 ctgaggaggg cgaagtggcg gagcaattag agttctttcc cgtccagagc ccgtgccggg
    28861 gaatttgcca gtctgacgaa cgcgggtttt gtcgcggctg ttttcgtagt cgtgatgagc
    28921 gttttaactg gaataaaatg agcgatggcg aaaaacaaga ggtgctgcgc ctttgtcggc
    28981 agcgattaat gcgcaaatta cgcgcaaata agccagcgtc atcagacgaa ccgaagcaac
    29041 catcactctt ttgaacctaa tcttgcgtat actcctctca tatctgataa gaggaagcga
    29101 ttatggttca gcgtattact attgcgccgc aaggcccgga gttttcccgt tttgtgatgg
    29161 gctactggcg attgatggac tggaatatgt ccgcccgcca gctagtcagt tttattgaag
    29221 agcatctgga tctcggcgtg accaccgtgg accatgctga tatttatggt ggctatcagt
    29281 gcgaagcggc gtttggcgag gcactgaaac tggcacctca cctgcgtgaa cggatggaaa
    29341 tcgtcagtaa atgcggtatc gcgacgaccg cgcgtgaaga aaacgtcatt ggtcattaca
    29401 tcactgaccg cgatcacatc attaagagcg ccgaacagtc gctaattaat ctcgcgaccg
    29461 atcatctgga tttgctgtta atccaccgac cagacccgtt aatggatgcc gatgaagtgg
    29521 cggacgcgtt caaacatctg catcagagcg gcaaagtgcg tcattttggc gtatcgaact
    29581 ttacgcctgc gcaatttgcc ctgttgcaat cacgtctgcc gtttaccctt gccactaatc
    29641 aggtggaaat atccccggtg catcagccgt tactgctgga tggcacgctc gaccaactac
    29701 aacaactgcg tgttcgtccg atggcgtggt cctgccttgg tggtggtcgt ctgtttaatg
    29761 atgattattt ccagccgctg cgtgatgaac tggctgtggt ggcagaggag ttaaacgcgg
    29821 gctcgattga acaggtggtt tacgcctggg tattacgttt accatcgcag ccgctgccaa
    29881 ttatcggttc aggtaaaatt gagcgcgtac gggcagctgt cgaagcagaa acactgaaaa
    29941 tgacccgtca acaatggttt cgtatccgta aagcggcact ggggtacgac gtaccgtaag
    30001 cacttttagg aatagccgcc gttcaaaaat gtgtcactgg tttacactta ttcaggaatg
    30061 cacaatgaac ggaggtccta tgaaacgttt tagtctggct attctggcgc tggttgttgc
    30121 aaccggcgca caagctgcca gtgaaaaagt cgagatgaac ctcgtcacgt cgcaaggggt
    30181 agggcagtca attggtagcg tcaccattac tgaaaccgat aaaggtctgg agttttcgcc
    30241 cgatctgaaa gcattacccc ccggtgaaca tggcttccat attcatgcca aaggaagctg
    30301 ccagccagcc accaaagatg gcaaagccag cgccgcggaa tccgcaggcg ggcatcttga
    30361 tccacaaaat accggtaaac atgaagggcc agaaggtgcc gggcatttag gcgatctgcc
    30421 tgcactggtc gtcaataatg acggcaaagc taccgatgcc gtcatcgcgc ctcgtctgaa
    30481 atcactggat gaaatcaaag acaaagcgct gatggtccac gttggcggcg ataatatgtc
    30541 cgatcaacct aaaccgctgg gcggtggcgg tgaacgctat gcctgtggtg taattaagta
    30601 attacgaggc cagcgtacct tgcggtggtg cttgctcaag ttgcgaaagc gagcagtaca
    30661 gccgccagac aattgccgcc agctcacggg cggcaggttg atgatgacgg gcaaggctgt
    30721 tgcaaatccg ctgtaattct tcaagtgtgg ccgccagtga tttttgctga acgccacgct
    30781 cactcatcac tccccgcaac agtgaaatac agttatcccg tactcgcgat aacggatcgg
    30841 aacgcgattc ccagtcgcgc aattgccaga caacatgaga acagttcagc agcactacac
    30901 cccagcgtaa taaccaacgg cgagccagcg catcctggct gttactcaac tgactgacgt
    30961 gatgataagt gagcgattca aattcgcttt cactcagtgt tggatggcgg cttagctgat
    31021 cgacaaaatc ccggcgcagc gcgcgaatat ggcggcggct tttacgagca tccgatcctg
    31081 gacgcagaat ggcgaacgct agccaagcca gcgcaacgcc aacgattttt gccagattat
    31141 cgttaagaaa atcagcaaaa tcatacaccg ggggattagt gacagcgata aacgaaccca
    31201 taaaaacaat cagttgcccc cacagtgcgg caaatttggg catctgcaat ttaagaagct
    31261 gcattgtcgc cagcagtgga aagagaaaca gtaaaaattg ccacagatcg ctaatctgga
    31321 ccatcaggcc aaatttgacc acaaagctga atagcgaaag taacaccagc gtgcgcatca
    31381 gcagtgacaa cgacttaaac ggtgctgcga cggcggagta gagtacgcag ctaattgctg
    31441 ccagcgttaa tgcattggca ccggcatccc attgcgatgc aatactccat gcgccaatca
    31501 tcatcaacgt acaaaatgta cgcagcccgc tccacatagc ttcggtgttg tcggtatgac
    31561 gagccagagc ggatgtccgt gcgagtctgg catgatcatc tacaccgctt tgcagacgat
    31621 gtaattcctg actactttgc agataaaggc ggcaaaaata acgtagtcgc tgccagaagg
    31681 cgacgtgccg gtagtcggcg acgttggtcg ggcgtagcgg ggcgataata cgtgcgacgg
    31741 tgtaagcatc tgtttgcgaa ctggcgagcg ccgtcagcaa ttgttcgaga atttctcgcg
    31801 tggcacctgg cggtgagggc cagttgagca acatacgtcg caggctggag atgacactgg
    31861 tcatacgtaa ttgctggtgg agtagcgcat taaggcgcgc gttttgctgg cgaaaacgat
    31921 agtggctcca gaaagcctgg atacgcagca aattcatggt cagtatctgc ccaatcaccc
    31981 cctcatgtgc tgcacgaatg gcatcgtttg tttcaggctg ccagagtaaa ctggcatgtt
    32041 ccagtaatcg ggcgtgcatg tttttcaatg cggttaaaag ggctgtagca tcggaactgc
    32101 taggcaggat catcatcatc atgccgccgc acaaaatacc gactatcacc tcgcaaacgc
    32161 gcgcctgagc gatatcccac agctggctgg cctcagtaat attaaccatc ggaaaggcga
    32221 tgatggcagc cgtgtagccc gccagttgaa atgcatacgc gacgttattc gtgaagtgcg
    32281 cacaggccca ggtacaaaag ccaagccacg ccgacatgct caatagaaaa aaccacggct
    32341 cattgagcgt atgcccggca agaagcagtg ccgcaatggc tcccagtaaa ctgccagcga
    32401 tgcgtccgag gcttttgctg atgacaccgc caacggtggg aaagctaacc actgcagccg
    32461 aggtcatcgc ccaatagggt tcatccagat tcaaataata ggcaaccgtc agcgccagac
    32521 acatggcaat ggtattgcgt aacgcataac gccattgcgc cagcgtggcc ttgaaccagg
    32581 gcaaattgcg caaggaccat gacgatgcgt tcattattga cctacggcaa tgctacaggt
    32641 ggtgcccgac accagcgtga catcgcctgg aactttatcc agcgcaaagc ggacgggaac
    32701 gcgttgggcg agacgaaccc aggggacgtt aggtttcaca tccggaataa ggcttgagtc
    32761 actttcaacg ctttgatcat aaatcgcccg accgatactg gaaacgtgac cccgtaacgt
    32821 tttgttatca ctatagaggg taatttgtgc tggtgcacct tcgcggatat ggcgcaactt
    32881 ggtttcttca aaataaccaa tgacgtaaaa cgagtggctg tcgacaaggg caaacagcgg
    32941 tttcccggtg tcagcgtaat cgccgatgcg ggtagtgagg ttagtcaccc aaccactcac
    33001 cggggcgcga atttccgttt gcgccagttg ccattgtgtc tgtttcagcg tggcctgtgc
    33061 ggcattaaca ctggcctgca tcgctttaac attgaggtta gcggtatcga gttcttcggc
    33121 agaaataaaa ttttgtgaga gatgacgacg acgattcgct tcgttattgg cttttgcgag
    33181 gtcagattgc gctttcgcca gctgagcctg tgcgttcagc tcggcgatct gaaagggcgt
    33241 tttatcgatc gtgagcagaa gatcccccgc gttaaccagt tggttgtctt ttatattcag
    33301 ctcaacaata cgtcccgaca cctgtggggt gatagaaacc ttttctgcgc gtattttccc
    33361 gtcgcgtgtc cacggagatt gcatgtaata attccacagc caccatccgg caagaaccgc
    33421 aactatcgct acaatgattg ttgagaaata cttaattgtt ttaattgaca tagttaccac
    33481 gcaatcagta ttgccagagc aaggcaaacg caaatcgcaa acagcgataa atccattaac
    33541 aagggatgcc agatgtcacc ggcgtagatc cagccacgaa gcaggcggtg tacgacaagc
    33601 cagatgacga atccaaacgc gaaggctttg aaaaacggag gaaaatagac ggacgcaccg
    33661 aacaccagat cttgtaaggg caatcctgtt gcattgagca taaacttcac aaagtagatt
    33721 cctttacgac cgaatatatt gcgttagatt aataaatatt ctttaagtgc gaaaaattta
    33781 cgcgcaattt ctgaaaaata tgtctgacgg taaccaaatg cagcaataca tttgttttag
    33841 caatacaatt gctgcacact attctaaaag ccgcataata tcttagcaag ctaattataa
    33901 ggagatgaaa ttggaatcgc cactaggttc tgatctggca cggttggtgc gcatatggcg
    33961 tgctctgata gaccatcgcc tgaaaccgct ggagttaaca caaacccatt gggttacgtt
    34021 acacaatatt catcagttac ctccagacca gtcgcaaatt caactggcaa aagcgattgg
    34081 catcgagcag ccatcactgg tccgtactct ggaccaactg gaagaaaaag ggttaatttc
    34141 gcgtcaaact tgtgccagcg atcgtcgggc taaacgtatt aaactgacgg aaaaggcaga
    34201 gccactgatc agcgaaatgg aagctgttat taacaaaacc cgcgcggaaa tattacatgg
    34261 catctccgca gaggaactgg agcaactgat tacgctcatc gcaaaacttg agcataatat
    34321 cattgagtta caggccaaag ggtgaaatga agggggcgtg tggtcagtaa cctgaccaca
    34381 cgtaaactta tttagcgcgg agaaacggtc acctgactgc cattgctggc cagtacgaca
    34441 cgttggcccg tagagaaacg agtgttgcct tgtttctgta ccaccatgat ggtattacca
    34501 tcgtctttac gaatttccag ctcgacaccc tgcgttttgt tcattgcact ctgtacgccc
    34561 tgaccagcta cgccacctgc aacagcgcct gctgcagtag ccagagaacg cccggttccg
    34621 ccaccaacag tattccccag gaaaccacca agaacagcac cgccaattgc accgataacg
    34681 ttggaatcat caccgccctg aatctgtacc ggacgtacgt taacgatggt gccatagctg
    34741 acattctgta cttgtttcgc ttcagaagcg gtataaacat cccctgacag ggtgtcgtta
    34801 ttaacacaac cgacaagaga cagacctacc attgaaacaa ccaatacgcg tttaatcatt
    34861 gaaaaatctc ctgttcacca tgaaacgcca cgcaagcatc cctcatggtc aaagtatatg
    34921 gcatatgtga atccatggtt acatgttcta ccaaatatga gcaaatcata atgaaagtag
    34981 gatcaaccaa ttataaacaa aacattcatg atgattattg atttgaagat aatgttttac
    35041 taccaacggt aaaaatcggt cattccgcat ggtattcacg aaaagtttat gtttgagtgg
    35101 tgcgcaaacg tcagattgaa aaaggatgaa ctatgaaatc gggccgcttt attggcgtta
    35161 tgtcaggcac cagccttgat ggtgttgatg ttgtgttggc gacaatagat gaacaccggg
    35221 tcgcacagct ggcaagcttg agctggccga tcccggtgtc tctgaaacag gctgtactgg
    35281 atatttgcca gggccagcag cttacacttt cgcagtttgg acagcttgat actcaactcg
    35341 ggcgactttt tgctgatgcg gtcaatgcct tgcttaagga acaaaacctg caggcgagag
    35401 atatagttgc gatcggctgt cacggtcaaa ccgtctggca tgaaccgacg ggcgtggcac
    35461 cacacacttt acagattggc gataacaatc aaattgtggc acgcaccgga attacggttg
    35521 tcggtgattt tcgccgtcgc gatattgcct tgggaggaca aggcgcaccg ctggtacctg
    35581 cgttccatca tgccttgctg gctcactcaa ccgagcgacg aatggtgctc aatattggcg
    35641 gcatcgccaa tctgtcactg ctcattcctg ggcagccggt tggcggctac gataccggtc
    35701 ctggtaacat gctgatggat gcctggatct ggcgtcaggc cggtaaacct tacgataaag
    35761 atgccgagtg ggcacgggcg ggtaaagtta ttctcccact gctgcaaaat atgctcagcg
    35821 acccatattt ctcgcaacct gcaccgaaaa gcaccggacg cgaatacttt aactacggct
    35881 ggctggagcg ccatttacgc cattttccgg gtgttgatcc ccgtgatgtt caggccacac
    35941 tggcagaact caccgccgtg accatttctg aacaagtttt gttgagcggt ggctgcgaac
    36001 gattgatggt atgtggtgga ggtagtcgta atccgctact catggcgcgt ctggcggcat
    36061 tactgccagg cacagaagtc accaccaccg atgccgttgg cattagtggc gatgacatgg
    36121 aagcattggc tttcgcctgg cttgcctggc ggacgctggc gggattacca ggaaatctgc
    36181 cttccgtcac tggcgcaagc caggagacgg tactgggggc tattttccct gctaacccgt
    36241 gatatcagag ttaactgaat ttttcttacc gcgtctgccg ttacacttgg aacgttaggg
    36301 agggcgtatt gccctccaga ccaggaaagt cttcgggaaa tgtctatgaa aaaactgtta
    36361 ctgatctgtt tgccggtgct tctcaccggc tgtagcgcgt tcaatcaact ggttgagcac
    36421 atgcaaaccg ctacgctgga ataccagtgt gatgaaaaac cgttgacggt caaactgaat
    36481 aatccgcgcc aggaagtcag ttttgtttac gataatcaac tactgcatct caaacagggc
    36541 atttcagcgt ctggtgcacg ttacactgac ggaatctatg ttttctggtc gaaaggcgat
    36601 gaagcgactg tctataaacg cgaccgcatc gtattgaata actgtcagtt acaaaatcca
    36661 cagcgttgag atttttccag gggcggcgca caatagcgtc acccactgac aatccgtaaa
    36721 gaaaaccatg tctgataacg acgaattgca gcaaatcgcg catctgcgcc gtgaatacac
    36781 caaaggcggg ttacgccgtc gcgatcttcc cgccgatcca ttaacccttt ttgaacgttg
    36841 gctctctcag gcgtgtgaag ccaaactggc agaccctacc gcgatggtgg tcgctaccgt
    36901 ggatgaacat gctcagcctt atcaacgcat cgttttactc aaacattacg acgaaaaagg
    36961 catggtgttt tacaccaacc tcggtagccg taaagcacat caaattgaaa ataatccgcg
    37021 cgttagcctg ctgttcccgt ggcataccct tgaacgccag gtgatggtga tcggtaaagc
    37081 agaacgactt tcgactctcg aagtgatgaa atattttcat agccgcccgc gtgatagcca
    37141 gattggtgca tgggtttcga agcagtccag tcgcatttct gcccgcggta tccttgaaag
    37201 taaattcctg gagctgaagc agaagtttca acagggcgaa gtgccattgc cgagcttttg
    37261 gggcggtttt cgcgtcagcc ttgaacagat tgagttctgg cagggcggtg agcatcgcct
    37321 gcatgaccgc tttttgtacc agcgagaaaa tgatgcgtgg aagattgatc gtcttgcacc
    37381 ctgaaaagat gcaaaaatct tgctttaatc gctggtactc ctgattctgg cactttattc
    37441 tatgtctctt tcgcatctgg cgaaaagtcg tgtaccggca aaggtgcagt cgttatatac
    37501 atggagattt tgatggcaag cagtaacttg attaaacaat tgcaagagcg ggggctggta
    37561 gcccaggtga cggacgagga agcgttagca gagcgactgg cgcaaggccc gatcgcgctc
    37621 tattgcggct tcgatcctac cgctgacagc ttgcatttgg ggcatcttgt tccattgtta
    37681 tgcctgaaac gcttccagca ggcgggccac aagccggttg cgctggtagg cggcgcgacg
    37741 ggtctgattg gcgacccgag cttcaaagct gccgagcgta agctgaacac cgaagaaact
    37801 gttcaggagt gggtggacaa aatccgtaag caggttgccc cgttcctcga tttcgactgt
    37861 ggagaaaact ctgctatcgc ggcgaacaac tatgactggt tcggcaatat gaatgtgctg
    37921 accttcctgc gcgatattgg caaacacttc tccgttaacc agatgatcaa caaagaagcg
    37981 gttaagcagc gtctcaaccg tgaagatcag gggatttcgt tcactgagtt ttcctacaac
    38041 ctgttgcagg gttatgactt cgcctgtctg aacaaacagt acggtgtggt gctgcaaatt
    38101 ggtggttctg accagtgggg taacatcact tctggtatcg acctgacccg tcgtctgcat
    38161 cagaatcagg tgtttggcct gaccgttccg ctgatcacta aagcagatgg caccaaattt
    38221 ggtaaaactg aaggcggcgc agtctggttg gatccgaaga aaaccagccc gtacaaattc
    38281 taccagttct ggatcaacac tgcggatgcc gacgtttacc gcttcctgaa gttcttcacc
    38341 tttatgagca ttgaagagat caacgccctg gaagaagaag ataaaaacag cggtaaagca
    38401 ccgcgcgccc agtatgtact ggcggagcag gtgactcgtc tggttcacgg tgaagaaggt
    38461 ttacaggcgg caaaacgtat taccgaatgc ctgttcagcg gttctttgag tgcgctgagt
    38521 gaagcggact tcgaacagct ggcgcaggac ggcgtaccga tggttgagat ggaaaagggc
    38581 gcagacctga tgcaggcgct ggtcgattct gaactgcaac cttcccgtgg tcaggcgcgt
    38641 aaaactatcg cctccaatgc catcaccatt aacggtgaaa aacagtccga tcctgaatac
    38701 ttctttaaag aagaagatcg tctgtttggt cgttttacct tactgcgtcg cggcaaaaag
    38761 aattactgtc tgatttgctg gaaataatgc attaagtgga aagggggagt gagaaatcac
    38821 tccccctggt ttttatacag ggaacatgat gaaaaatatt ctcgctatcc agtctcacgt
    38881 tgtttatggt catgcgggta acagtgcggc agagtttcct atgcgccgcc tgggcgcgaa
    38941 cgtctggccg ctgaacaccg ttcaattttc taatcacacc caatacggca aatggactgg
    39001 ctgcgtgatg ccgcccagcc atttaaccga aattgtgcaa ggcattgccg ccattgataa
    39061 attacacacc tgtgatgccg tgttaagtgg ctatctggga tcggcggagc agggtgaaca
    39121 tatcctcggt atcgtccgtc aggtgaaagc cgcgaatccg caggcgaaat atttttgcga
    39181 tccggtaatg ggtcatccgg aaaaaggctg tatcgttgca ccgggtgtcg cagagtttca
    39241 tgtgcggcac ggtttgcctg ccagcgatat cattgcgccg aatctggttg agctggaaat
    39301 actctgtgag catgcggtaa ataacgtcga agaagcggtt ctggcagcgc gcgaactcat
    39361 tgcgcaaggg ccacaaattg tgttggttaa acacctggcg cgagctggct acagccgtga
    39421 ccgttttgaa atgctgctgg tcaccgccga tgaagcctgg catatcagcc gtccgctggt
    39481 ggattttggt atgcgccagc cggtaggtgt tggtgatgtg acgagcggtt tactgctggt
    39541 gaaactgctt cagggggcaa cgctgcagga ggcgctggaa catgtgaccg ctgcagtcta
    39601 cgaaatcatg gtgaccacca aagcaatgca ggaatatgag ctgcaagtgg tggctgctca
    39661 ggatcgtatt gccaaaccag aacattactt cagcgcaaca aagctctgaa atatctcatt
    39721 tcggcccaca acgacctgtg ggccgatgga tattacttta agccttccgc tgacagcgcg
    39781 tcttgtactt ccggacgttc agccatacgt tgcataaatg ctgcaatgtg ctctaaccct
    39841 tccagattca gtttcaccgc gtatgcccag cgcagaaccg taaacagata ggcatcagca
    39901 attgtaaatc tttgcccgca gatccagtgc tcatccttca gtgcctcgtt cacatattgc
    39961 agcttcttct ccagctgcgc gcgaactgtc ggtttgtact cttccggtgt atcagggcga
    40021 aacagaggtg tgaaaccttt atgcagctcg gtggcgatgt aattcagcca ttcgatggtt
    40081 ttatagcggg aaatactgtt taccggtgcc agcaactggc ggtcggggac gctgtcggca
    40141 agatactgca taatcgctac gccttccgtc agcaaagtac cgtcatccag cagcaatgca
    40201 ggcacctgcc ccttagggtt aacgacaaag taatcgtcac cgttttcgag acgttttttc
    40261 attaaatcca cactgacgag ggtaaaatcc tttccgctct cacgcagggt gatatgggaa
    40321 gcgagagagc aggcacccgg tttgtagaac aatttcatcg gtaactcctt ttgtctgtgg
    40381 tttaaagtat ggtagtgcgt tgctgaacaa aaaaaaagcc gctaacgaaa agttagcggc
    40441 ttgtaaaaga gtttccctga attttacgct acagcagctt tcgccgcttt gtctgcagcg
    40501 tcatcctgcg tcatacggtg cagtttaggc gcggtcagca gcatcagtac tgcaataatg
    40561 gcagtagcga caccaatctg caagaatacg cgaccataaa cttccagtga catcagcggg
    40621 tcggtaacgt tatccggcac agccatcata cccgcaacat aaccaccaat caggtttgca
    40681 ccggcagtgg tcaggaacca gctacccata atgaagccca tcagacgctg cggaacgagt
    40741 tgtgcaacca ttgccagacc cagaccagag atcatcagtt ccccgatgct ctgcaggcca
    40801 tagcttgcga ccagccagct tacagacacg ataccagcgt cagacgcgaa tttcgcaccc
    40861 agcggcagaa tcaggaacgc accagaacac atcaccatgc cgattgcaaa cttggttggc
    40921 atcggcaggg tatcgcccat cttgttatag atagcggcca gaatcggact accgatgatg
    40981 atccagaacg ggttcagtgc ctgatactgc tcaggttcta cggccagacc cagaatggag
    41041 tgctcaacgt tacgaatcgc aaagaagttc agtgacgttg gcatctggct gtacagcacg
    41101 aagaagataa tggcttcgag catcaggatg aaggcaacga tcattttacg acgcgcagca
    41161 cctttcatcg cgaaggcttc tttaccgaag ataaccacga taccgaaggc aacaacgccc
    41221 agcgccatac gcgcaacttc ctgattgtgc agcagccagg tggcgatagc gatcagtgcc
    41281 acaacaccaa taatggtcag cagcaggtta cggtagttga taggctcgaa gtctggtttt
    41341 gaaccgtact gtttaaccca gcgttggcag aaggcgaagt taacgatagt gatcagcagg
    41401 cctacaacgc tcaacgcaaa cgcaacactc cagccgtatt tcgcggccag ccacggcgta
    41461 gcaatcatag agaagaaaga gccgatgttg acggacatgt agtacatggt gaatgcaccg
    41521 tccagacgcg ggtcgttttt ctcatagcat gtagaaagca gagaagacgg gttagcttta
    41581 aacaggccgt taccgaccgc aatagccgcc atacccatat aaacgatacc ggcgtcgtga
    41641 ccagaccagg caaccagagc ataaccaatc gccagcacaa tagcgccgag cataattacg
    41701 cgtttagtac ccagtacctt gtcacctaac cagccgccga tagcgaccag accataaacc
    41761 agggcactaa aggaagagaa aagggtgatt gaatccgctt cagacatacc cagttgttta
    41821 accaggtaaa cagccataat tccttgtagg ccgtaataac caaaacgttc ccataactca
    41881 atcgagaaga tgagatagaa cgccttcggt tgtttgaaag cgttcaaact gacgctttca
    41941 gttggttttt ggtttgcagt ggacactttt acctcttttt ttacatccca tattaacggg
    42001 ggtgctttct cgcgttatgt ctaaaaaaca tacgcgcaat tgttatagtg ggaggggaaa
    42061 cggcaggtaa tgttcactat cttgcacttt cagacaagtc ctttgtaata atctgttaca
    42121 tataactgag gcggggaatt caaccattcc gggaaatgtt atccagcgtt aaatttttca
    42181 aaaaaacgta actgcagatt ttttcactgc actgagaggg gggtgatggc ttaacggcga
    42241 tatgttctgc tatttagtgt ttttgctagt gagatagtcc agtttctgaa aaatagccag
    42301 tgtaatgttt tgtaggtcaa tatttgttgc gtgtgtaaat ttaaatgatt ttaacctgat
    42361 tcttgcaatg tttttacatt gcgacagcat cgtgatcttg atcacgaatg aatagaaaat
    42421 ttcgctatta aaaaaaacaa ttaacctgca tttttggtta tctgcaaacg gattacctga
    42481 tgataacgct aagcaatggc atcattcacg ttaaaaagat gttttaaaag aggataaaga
    42541 aaggttatca atggggtaat cggtgttacc ccttttcttc agatgtcaac tttctctttg
    42601 tattcacaaa gatcttcaat aatacaagag ccacagcggg gcttgcgggc aatgcaggta
    42661 taacgcccgt gcaggatcaa ccaatggtgg cagtcgactt taaactctgc cggaaccact
    42721 ttcagtagct tttcttctac ctgttcgacg tttttccccg gcgcaaattg agtacgatta
    42781 caaacgcgga aaatgtgcgt gtcgacagca atagtcggcc agccgaatgc agtgtttaat
    42841 acgacgttgg ctgttttacg acctacgccg ggcagggctt caagcgcagc acgatcttcc
    42901 ggaacctcgc cattatgctg ctccagcaag atacggcagg ttttgatgat attttctgct
    42961 ttgctgttat aaagcccaat cgttttgata taggttttca ccccttcaac gcccagttca
    43021 agcatcgctg caggcgtatt cgccaccggg tagagtttcg ccgtcgcctt attaacactg
    43081 acatcggtcg cctgagcgga aagcagtacg gcaatcagca attcaaaagg cgaactgaaa
    43141 ttaagctcgg tggtgggatg aggattgttc tcacgcaggc gagtgaggat ctccaggcgt
    43201 tttgctttgt tcatcagaca ttccctgttt caccgtttgg caatgcacgt tcggcagttg
    43261 cttcagcacg gcgctttttc attcttttat caatcaggta ttttcctgcc agcatcagtc
    43321 ccaggccaat aaatgcacct ggaggcagca tcgccagcag gaaaggggag tcggtgtgga
    43381 aaatttccac gcgtaatact tttgcccagc tacctaacag cgcatctgca ccgtcaaaca
    43441 atgtgccatt gccgataatt tcgcgtagtg aacccagcac gaacatggcg cgggttgcgc
    43501 ccataccaat tgaaaagccg tccagtgccg aaagcgccgg accttttttg gcggcgaagg
    43561 cttcagcgcg gcccacaacg atacagttag tgacaatcag cggaataaaa atccctaatg
    43621 attgatacag gccaaaggcg taggcgttga tcagcatctg tacagcgctg accaccgagg
    43681 cgatgatcat cacgtaaatg ggaatgcgga tctcggctgg cgtccagtga cgcagcgtcg
    43741 aaatggtcag gttggtcagc gtcagtacca gcgtagtcgc aagtcctaaa cccagagcgt
    43801 tagtggcagt ggacgtgacc gccaacagag gacaaaggcc gagcaactgg accagcgcag
    43861 agttgttttt ccacaccccc tgaacaataa cgtctttaat ttcgctcacg ctttattctc
    43921 cacaggcagg aagttgagaa agttgtgccg gtaacgtctg agcgtacaat ccggcgcgtt
    43981 ttaccgcatt aaccaccgcg cggggagtaa tcgtcgcgcc ggtgaactgg tcgaaatcac
    44041 caccatcttt cttcaccgcc cagtgcgcat catccgcacc actgattttt ttacccgcaa
    44101 aatgggtgat ccagtcagaa aggcgcagtt cgattttatc gccaagccct ggcgtttcgt
    44161 ggtgctctgt cacgcgcgtg ccaagtaccg tgccgttaaa atcggctccc accagcagct
    44221 gaatcgcacc ggaatagcca tctggcgcgg ttgcttccag aacggcggct accggtttgt
    44281 catcctgttt ggcgatgtaa acccgatgct cacctttacc taactctggc gcagttacca
    44341 gatagcaact ctgtgccagc gcattgttat agcgttcggc tggcagcacc tgatcaaata
    44401 atgcctcttg ttgcagactg gcctgttcag caatcgtcgt tttggtcatc tggttgatgg
    44461 ccgcagttaa ccctgttgaa cccgctgcaa atagcgctaa cgtaatgccg tgttttcgga
    44521 tagttttcag catggtttac cctttgcgat ggccgtagac gcgcggacgc gtgtagtaat
    44581 cgatcagagg aaccgtgatg ttcgccagca ggacggcaaa agccacgccg tcaggatagc
    44641 cgccgaaact gcggatcaac cagactaata agcccgccag cgcgccgaaa ataagacgac
    44701 cacgattggt cgtagaagcg gtaaccgggt cagtcaaaat aaagaatgcg ccgagcatgg
    44761 tcgcaccaga cagcagatga atttgcggtg ctgccagtgt ttctggtgag aacaaccagc
    44821 ccaacgttgc gcataacgcc agcgttacta agaagctgag gggaatatgc cagcgaatcg
    44881 ctttctgcca tagcaaccac aggccgccag ccagccaggc gagatttacc cattgccaac
    44941 cagcgcccgc cagaataccg ctgtagatcg gatattgcat aatctgttca accgaatgac
    45001 cggcacggac agaggtttta aatgtatcca gcggtgtcgc ctgactaatg ccatcaatac
    45061 ctaagcgtag tgtgttcata tcaccaccac tggcggtatg tccgctgaaa ataacctgta
    45121 tggcgtcgat aaaaccaggg atgttgaccg caatttcatg tggcggtaac cagctggtca
    45181 tctgcaccgg gaaggagatc agtaagacca cataaccaat cattgccgga ttaaacgggt
    45241 tttgccccag accgccatac aactgtttag cgatgatcac cgcaaacacc gtacccagca
    45301 cgaccatcca ccatggcgcg agggggggaa tacttaccgc cagcaataag cctgtcagca
    45361 atgctgagtt atctttcaac gttgcggcta ccgactgctt gcgtagtttg agtacgagag
    45421 cttcggctaa cagagcacta accgatgcca acaggatctg aacgagagta ccccaaccaa
    45481 aaaaccacag ttgcgctgcg attcctggca cggctgcgag caacaccaac agcataatgc
    45541 gcgatgtctg gcgctggtta tgggtataag gggagctagc tattctgaat accatttagt
    45601 cctcgtttac aaccttctgc tgggcggctt ttttggcctg agcgcgggca atagccgcgg
    45661 caactgccgc tttgcgcgga tcaacctgtt cttctggttc tgcgttagtc tgttgctgtt
    45721 ccagcttgcg cgctttggca cgggcaatag cggcttcgac ggcggctttg cgcggatcaa
    45781 cctgttcttc tggttccgca ttagcctgtt gctgttccag tttgcgcgct ttggctcggg
    45841 caatagcggc ttcgacggcg gctttgcgcg gatcaacctg ttcttctggt tc
//
LOCUS       DAZGVQ010000019        44906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000019 DAZGVQ010000000
VERSION     DAZGVQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.019"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            142..882
                     /locus_tag="ACHLAG_001035"
     CDS             142..882
                     /locus_tag="ACHLAG_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654311.1"
                     /translation="MSQIFFDTIDNDQYDFMTEWNTAVMDKWVAENIGLSRCKDEAEL
                     FETKWFDYRDMHPLMATCLFTEAYKRQYSNIMLTHGREHFETAPFTTGLKRLPYQELS
                     TANKTSLWKARQFADRYCCSYDYFISTVLSAAARRLWDKLPRPQHLWQPELIEIFEDK
                     LARRATTRLDDSLVSFKHMGDMQFNPIQESYFEWILERLRTIPRSKRIRAIFSAIWLM
                     EIVPERLISAHFPEELEEARRFIDPLSN"
     gene            926..2266
                     /locus_tag="ACHLAG_001036"
     CDS             926..2266
                     /locus_tag="ACHLAG_001036"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFP0654312.1"
                     /translation="MTELCHTGRGLSEEFDEDFQNRLTAYFCRDHEFLTRAGDLVVPS
                     QFANAANAILVNMVSGYYRMYKSAPSSSAILDMLKRAKRDKTIREELFADVVAAFKRI
                     LAEKLSDTSYMVDQVSTFAKSVAFDDALIKAAELKEKGDFQGAMAIMAKVQQIGSNEA
                     TGIYDYYTSASERLKAREYEASEEYVPNSITTGLPLLDRLLYQKGWAKREMVLFMGFA
                     KSGKSTAMGEFSINATLAGYNVLYLSLEVHTTILSDRFDARLSETEMSKLVERRDEVH
                     RKLAELGATKGIGSLWVVERPSGSMSPADLDRMLNSMKANGMVPDMVVVDYADLMRAS
                     YDLRDDRANIRSIYTDLRALYDKHNVAGITASQTNREGGASEVATMMHAADNIEKVRI
                     ADLVITINKTEEEEAKGEARLYFAGSRNQQGGISIRVKQNLEQMRFIERILDVT"
     gene            2414..3526
                     /locus_tag="ACHLAG_001037"
     CDS             2414..3526
                     /locus_tag="ACHLAG_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP0654313.1"
                     /translation="MSELKELITELDFEQWLDTEGIVYRRGGVSARGREVNIKECPVC
                     GSSNWKVYFNLTSGVGKCFAGDHPEEIQFNKLVFLKHYSGKSRRQFEEYVQNALLSQG
                     WAPKKEEIVLASTVELEGPLALPRHYELPIDGRLPDYLVERNISPEMAKYFDLRYCVE
                     GKHAYVDPYTDQVKGQIFDMRILIPVYDLDGVMKTFQGRDITGTAERRYLFPMQLPAS
                     GKFLYNGHNAVGKQTVVVCEGAFDVMGVKRAIFDEETLRDYVEPIGTFGMHLSGNTTQ
                     DAEDQLGAFLTLKARGLRNVIMMWDSEKQAIRNTMAAARRLTSIGLNVKVACLGEEGL
                     DPGDATPEQIIKAYYRAKPYTKQLELQSKVLGIKAL"
     gene            3605..4381
                     /locus_tag="ACHLAG_001038"
     CDS             3605..4381
                     /locus_tag="ACHLAG_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654314.1"
                     /translation="MKDEIQKLACDIIDKTGLEISESNRLDIIEKAVKTAMDHIATRL
                     VEIPLPGLPYLKVELHVWGESSCSRRSALVVFISKENPLSLKVQVGAWMDGKVIYTNT
                     VFCLSNDETIEAAIQEAVLAMRSSGLMKNNYEEYLRSISGEKTLSLKADFVTPKNLLE
                     VLLNKGANDAVNVIRESEYASLCDMCKSQLDLVHIVIDAGKACDGVMAEFAWKVVRIA
                     NELPMIEQEAKSYATNHVTELLVPYRLESNQRKMVSWGSW"
     gene            4437..4919
                     /locus_tag="ACHLAG_001039"
     CDS             4437..4919
                     /locus_tag="ACHLAG_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654315.1"
                     /translation="MAVVVKADLSKIPSISGNNGYSLRCEEVKINGESAYCSYSVCQH
                     TILAFKENRLPRTSFQSCATAIKAGKCKALKMMVEEIRKGESLYFEDMTALIKEVEER
                     NKQARTIKRKRDSVTINSMVKKSTTSQTAITDVYAALLEETTKETHEQIDQHMEAKQQ
                     "
     gene            4916..6238
                     /locus_tag="ACHLAG_001040"
     CDS             4916..6238
                     /locus_tag="ACHLAG_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFP0654316.1"
                     /translation="MKKLIALKHKLDEMKAMGTNAKKEALANMDDFEQSMVSLMLNPF
                     IRFGVKKYKVAEPLSESIPSDEKAIDVLNKLASRELTGNAAIAAVESIVASMCADGQD
                     VFRRFLLKDPKAGVGISLCNKVFENPIPKFEVQLASPYKEKGDKYPFKPNPKAKWPMI
                     GSLKLDGLRVICEVIVDEEEVNFLSRTGNPITSLDHLKPAMLELGKLSGHKHIFFDGE
                     GTAGSFNQSVSALRKKNVQAIGAIYHVFDFFLPEWRAQAKSKEYAKTGMKLKERLAIL
                     VALFKNDRSEDYAQDIHLHPFYIIHSHEDFIERFMKRLDDNEEGEMGKDPNSVYEFKR
                     TRSWWKLKDEDSEDGEIIDFEPGDPDSGFANTLGKIVIRLENGVIVRASGIKHKYLDE
                     IWNNKEKYRGRIVEVHCHEKTPDGSLRHPRLKWPRCLRDTEDRIGDKE"
     gene            6235..6450
                     /locus_tag="ACHLAG_001041"
     CDS             6235..6450
                     /locus_tag="ACHLAG_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654317.1"
                     /translation="MIVLSKREKETLHEISKWPEFPEYWKPKTRAKLERLGLVANVSE
                     TWCSANYQLTDKGKVLLQQLVESGALK"
     gene            6596..6886
                     /locus_tag="ACHLAG_001042"
     CDS             6596..6886
                     /locus_tag="ACHLAG_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type natriuretic protein"
                     /protein_id="HFP0654318.1"
                     /translation="MSNLTSWDWWLATYFLAAGVAFAFYVGQLVVKLLLIKFASHKRI
                     DDGLWRLGTLVETRYGQLKENETITIQAKRFTATITRTPSRRVALIKKVTTE"
     gene            7665..7739
                     /locus_tag="ACHLAG_001043"
     tRNA            7665..7739
                     /locus_tag="ACHLAG_001043"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7697..7699,aa:Asn,seq:gtt)
     gene            7745..7831
                     /locus_tag="ACHLAG_001044"
     tRNA            7745..7831
                     /locus_tag="ACHLAG_001044"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7779..7781,aa:Tyr,seq:gta)
     gene            7836..7911
                     /locus_tag="ACHLAG_001045"
     tRNA            7836..7911
                     /locus_tag="ACHLAG_001045"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7869..7871,aa:Thr,seq:tgt)
     gene            8232..8318
                     /locus_tag="ACHLAG_001046"
     tRNA            8232..8318
                     /locus_tag="ACHLAG_001046"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8264..8266,aa:Ser,seq:tga)
     gene            8540..8890
                     /locus_tag="ACHLAG_001047"
     CDS             8540..8890
                     /locus_tag="ACHLAG_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HFP0654319.1"
                     /translation="MTSKENTPLKVTPEHIEQLIQSEHYFTAYDASHGDNFISVYNSK
                     TDIDKGHESLKLLTFCVMVLKNGYTVTGKSACVRPEIFNFDVGREYARKDAIDQIWPL
                     EGYLLKQKWHEAKQ"
     gene            8887..9132
                     /locus_tag="ACHLAG_001048"
     CDS             8887..9132
                     /locus_tag="ACHLAG_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxA family glutaredoxin"
                     /protein_id="HFP0654320.1"
                     /translation="MTITIYGRDNCSYCKRAVELAKQLKGHGYGDYEYIDITTAGIDK
                     EKLSEIVGKPVETIPQVLIDGQPIGGYTELAAYVSTL"
     gene            9343..10446
                     /locus_tag="ACHLAG_001049"
     CDS             9343..10446
                     /locus_tag="ACHLAG_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0654321.1"
                     /translation="MKNSFDRARAAENTSKEAIEYLERASQMQAVMISQVSNDMRFSD
                     AFMLFTRLSLLITRRRPEIAVHCILIHVLPHIADVKVSDINRFMVNQLVNPLILDGKI
                     VMGRRVFSLMKQFLSWCAFQGMIDVSPLNDMSLNKVAGGAKPTPRERKLTDAEVWVFW
                     NIWDYFNVCAGTKWAARLCLVSARRPDEVLRAKKSEFNLKRGVWNQGKRNKSAREHSL
                     PLSTLMRTCIEELFEYGKGSQWLVPSNKKIGKDLPMSKVAIAQALRRILERPELMELE
                     PFTPRDLRRTARSYFPALGISQEVSRKIMNHSLEGIDRVYDRHDYMDEMRDALESFST
                     YIASIVEQPDLDEIDHKFKGDRLSTELIRVNFS"
     gene            complement(10441..10827)
                     /locus_tag="ACHLAG_001050"
     CDS             complement(10441..10827)
                     /locus_tag="ACHLAG_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP0654322.1"
                     /translation="MSDNKIEFIESRYAAFIAGLIESSPMSQAQIARLIGYKNANNLS
                     LIKSGKIPLPIDKVRPLALALGIEPSRLMMMVLEERQPELAAFLYKEGTAPLNEDEKQ
                     VLAAYNERFGKEKGASQKVVEAIKSL"
     gene            11079..11291
                     /locus_tag="ACHLAG_001051"
     CDS             11079..11291
                     /locus_tag="ACHLAG_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654323.1"
                     /translation="MNTTISSLIALEIGHVQKLADECVADILTDLPNEQIQVGVNDTT
                     GFIFEFNNKRFTLLNTGSGSLAVRIC"
     gene            11390..13498
                     /locus_tag="ACHLAG_001052"
     CDS             11390..13498
                     /locus_tag="ACHLAG_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobT"
                     /protein_id="HFP0654324.1"
                     /translation="MSKKRSIKEVQDFRDSVKRVVALLSGKNIPVAERGDDAYVRYND
                     DGEPILVNIPSIPDNATPALMNAVRGFLDHEVAHILFTDIRVSNKMREKGRVPSWSLW
                     NALEDVFIERKMGEVFNGTRRNLMATQRLIIEKVFKPKASEAIAYCDKDQRALFLNFF
                     LCPVVRAWDGQAPFVDFMDEYWPVIEKPISLLKEHGIDVAVRNMSCTEDCVKVAATIA
                     KILKDTESESKGKESAPGKTSDPSDADQTDASGENSEDNEDHETPSVLDNHKSIKSES
                     HSKHKHDNNDGDDSDNSESSETIFDDTENDKEVSDSDASDNAASESLTADHEKRKTTE
                     DGSSDIPTPSKMSLEEALEELDSMEDEVGGMTEDALSETIKSELTESSKSEYRPYNRS
                     YDFIGSIDQAEAHIKRLIKTFSDIDLGGYPISRYRIVPEGNQLFDKYIEKHLSSGVSS
                     TLAKDLERAIASRNRVQFIPGQRRGRIHGSSIYRLAMNDARVFRKKEESKAVNACVQQ
                     VIDLSGSMSGITIKLALASAYTIADALDRINVPNIITGFTTFGSHMAAGELKAVKYEF
                     SRFESLMLPIIKNWNEKVNSREVRSRMGCVGYTFPLLNNVDGESIASLASLFSGRMED
                     RKIMLVLSDGAPWAVGRGFDAHLRSVAKQIETQTDIDLMAIGIMTDAPERFYSNHALV
                     TSVDSLGSSVVTELSRIILK"
     gene            13594..14829
                     /locus_tag="ACHLAG_001053"
     CDS             13594..14829
                     /locus_tag="ACHLAG_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0654325.1"
                     /translation="MTTTALQNEKNPSDYLVCKWCGKSFHYFKSHVANGNCEGIPESV
                     KDADPDTVLKMYTTQFPDEPTLSKKALDAIQAKRAEQKSEMAKSSGLTSSPGYTGTVE
                     YKTDLVAAHELLNVTVEELGTKRGTPLMVSVNVNTPYPEFVPEVKKGYVYGDFELIKD
                     IFMMLELGIPGYLWGHAGTGKSSLPTQLCALLNRPLIRAQHTASMEEAHVTGQILARD
                     GSTYFEPGLLALAMKHGWVYLADEYDFAFPQILGVYQPVLEGEALVIKEATPEWRRIT
                     PHERFAFIGTGNTNGSGDETGLYQGTNIQNAANFSRFGIVSNVKYMSKDAEINMLTNA
                     GIVDEYAEKMVKFAGIVRDGYEEHLISQPIGPRELLLSAKIGMMRGDFVTGIERSFIN
                     KLPSASAQAAREVVQKIFG"
     gene            15010..18516
                     /gene="dnaE"
                     /locus_tag="ACHLAG_001054"
     CDS             15010..18516
                     /gene="dnaE"
                     /locus_tag="ACHLAG_001054"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP0654326.1"
                     /translation="MKALMVRTDFSLGESALKAENAVKIAREAGYTAVISADSMNIAS
                     VIPLQRAAGDDMAVICGVKLNIVDDPTYEHRAKLAKESMRCMESLERGRNYSFTALIK
                     NEKGYRDICELMTAANTREQFYFVPRLSLEQLVSTYAKGNIILLTSDIGSVFQRNDFA
                     KIISTLITAGGKDNFYSVVYPHPTPFYDQINVRAMKVASALKIEPVAFYPAYYESIDD
                     ADIKDIAHMVTNNIKIDQPHRLRIPHQRDNAVNGRRHLLEALKAFSVRMDVPVTAAMA
                     STTQDTIIDACTWRWHELPPALPKMADDEPATLMKLAVAGLRKRLTTKEFGYTPPASE
                     NRVYVERLKYEMDTLTRLGFCGYFLMVRDLMNHSRETGIPVGPGRGSSAGSLVAWCIG
                     ITNVDPIRHGLLFERFINPERLDLPDADLDFSQARRHEVIEYLNERYGEDYVAGIPNF
                     TYLGAASALRDTARIYGVESADMAVSKELKNVEDDSLPLEELREQLASLDKYATKYPD
                     AFNAACKLQSLMRGFGRHAAGMIVAGVPLTERTPVERRGDARCIAFDKRYCEAMGLIK
                     LDVLGLATLDLLDSAKRYIKENTGEDINLDAISLEDRKVLDGFAAGYTQGVFQLESGP
                     MRKLLKDLGGGIEPMSFKTVVATTALFRPGPIQSGMLDDYVSVAKGFMAPHSIHPRLE
                     EVTRETNGVLLYQEQIMQSSRVLAGFSMAEADALRSAIGKKNMDKMKAIGSDFVERAQ
                     AGWVTLSLKNGGTVEVHKHAKLDCSDGKRRSYSEAISDGIDYVEIVSEQEGLSKEKAQ
                     EIWDAFEKFGGYAFNKSHSVAYSLISYQSMWLKTHYPAEFFAAALTILGEDKHQGLVK
                     DALTYGIRVLPPDVNVSSNRIEIRTLEDGSQVLYAPFSAVKGCSENGCQAIMRAREKV
                     GGKFESLAQFEEAVEKRACNSRVRESLQKVGAFASIEPGSLPATDPERLRDQAELMGN
                     LVIDAVKASRPFEMNPKRSAEINVLMTRMAAEMGLGDELIRPSIGIKPKIMIILDNAN
                     GNDARTGYFMENGYDDFKAKLLTAGDLRMGDLYVTGVCKKVKDKEKDYTKDEIGQFTD
                     FMREEINLVRPTYVLTCGSRATALFNNKNKPSDLVGRKEYLPDLDVTVFYGFNPNILY
                     FRPEEGERLEAILADVAETIKT"
     gene            18642..19073
                     /locus_tag="ACHLAG_001055"
     CDS             18642..19073
                     /locus_tag="ACHLAG_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654327.1"
                     /translation="MITDIYEKIMSDLEFDRDNLEEVWRRQPRLLMEYGSKLAHAERS
                     VAEAKLNLEAVEAKLYDTERKNLSMNGIKFNESVLDAKVKTNPQYLSKRQKLDEARHI
                     ADIYKHAVAAFSHRRDMIVQASKMAIVELERLGSERFITPR"
     gene            19191..20219
                     /locus_tag="ACHLAG_001056"
     CDS             19191..20219
                     /locus_tag="ACHLAG_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFP0654328.1"
                     /translation="MSKTLLDLLNKTREDIAAKRGNNVDLTRLKDGVNYIRIFPNKDD
                     PNGKFFQTFGMHYVKYQNEEGKEATNAYICEQHTHGRACQLCEMVMEGRARHKGNKAM
                     EERIGQMRATPRYLVNGILSAREDFADAEKCQLIELPSTVFDDICKAITEDIADDIGN
                     PLSKEEGYAFLIKRTGSGRDTKYDVSPKRKVYKGDIEDKFWNTQHDLIAYANQADETR
                     LLSTVRTMGRLIGIAAPTAAASAPAISSTAKTSAAALPGFGSVTGHTEGATAVATAHT
                     PASEPTSLVDEEILRAVETEFKPEASPAAVAVSVKESEAVAATSVAAASATEDEGLDD
                     LLRELDSL"
     gene            20280..21224
                     /locus_tag="ACHLAG_001057"
     CDS             20280..21224
                     /locus_tag="ACHLAG_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HFP0654329.1"
                     /translation="MNYLFVDGNSLGYYHQQSDKLHNGEMEVQAAFGFVKNVRRYASI
                     LHARPMILWDGFSDKRRDFYPDYKANRDDDPDMKKMKEGFDIQKPYILKMMTALGVTQ
                     LIAKDAEADDLAGLLVSRMAPQPTVEHIYLLTGDSDWLQLVRENVSWVSLREDAKNKQ
                     VNFEQFAELTGFATPRAFLEAKALQGDKSDNISGVGGIGAGGAKELLHEWGSVATMVR
                     GINDGSIVVDKGRHKTAFNKLAKNAFNEKTGCRMLEAFKRNITLMNLIETKFPPTEIE
                     TIKGNRDVKAFEQLCYELNFRSFLEDLEVFVLPFERYC"
     gene            21224..21490
                     /locus_tag="ACHLAG_001058"
     CDS             21224..21490
                     /locus_tag="ACHLAG_001058"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654330.1"
                     /translation="MLKSIINGATTTPTQLAKEIVFYHGEYAVIALPSILGAAGMKAT
                     DREFGLVSEQVVKILARVSRLLNHDAIVFDESAALKRINETKGA"
     gene            21493..22566
                     /locus_tag="ACHLAG_001059"
     CDS             21493..22566
                     /locus_tag="ACHLAG_001059"
                     /inference="COORDINATES: protein motif:HMM:NF012381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP0654331.1"
                     /translation="MAKGKSALALALKKKIGSNDEIQKVTHWIDTGFPPLNKAISGRY
                     DGGFPCGRIVEVFGPPSAGKTFLATAAMISAQKQDGLAVFLDHENSFDVGLAVANGLN
                     ADEDDGQWVYKQPDTFEDSVELIGTILKLVRDEELIPESAPICIVADSLASMVPNSKA
                     EKFEKMAEGTAKDKDQLNMNDNTALARATSANFPTLALWARKYNACIIFLNQVRTKIG
                     VMFGDPTTSPGGDSPKFYASVRIRLGASVMKDGKEKIGQDVGAECIKNKVAPPYGKCT
                     WKFYFDPTRGLDVIESLVEYMLEEGYLPKNASGRVEIGDKKYTKSQIVEMYREKPLAE
                     IIAALQAIDDRRAKDNPTESVEE"
     gene            22660..22860
                     /locus_tag="ACHLAG_001060"
     CDS             22660..22860
                     /locus_tag="ACHLAG_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654332.1"
                     /translation="MIKIYLLAVATGVSVALIYGLLVPSLISTKSDLAVMFGVIVGFG
                     APVIGLIAGRKFINSLIKAKGK"
     gene            22864..23694
                     /locus_tag="ACHLAG_001061"
     CDS             22864..23694
                     /locus_tag="ACHLAG_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP0654333.1"
                     /translation="MKKGLLAVALAAICTMGLTGCDRVEPGYVGIKVNKLGEDKGIGE
                     VVGVGRQWTGLNTELYVFPTFKQMKTYDEPFTFQMSDGTAIGHKIGVAYLVNRDKVTT
                     VFQTYRKGVDDITESDLRQKIADSLNRLASRMTTDSFIDGGKAQLLDNALKDIQKEMS
                     PVGIEVLSLSWVGKPDYPKTVIESINAKVTANQRTLQRQQEVEQRKAEANMLREQANG
                     EADAIRARAQAEADAIRLRGEALRQNPNVMELEAINKWNGQLPQYMTQGANTPFITVK
                     "
     gene            23786..24211
                     /locus_tag="ACHLAG_001062"
     CDS             23786..24211
                     /locus_tag="ACHLAG_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654334.1"
                     /translation="MTAIKKLYDAANVALDVIDDEAAKGFPEPDWAHQLRNAIAEMTP
                     PDPTPEETDWQRFIRMYAQEIGPTPTAEQAMLLKYFKEAGEDLPIDDSAYWFHCAWRK
                     YDVIFTQGMGSKDMVVWHLLHIDTAVDRVIEQFFPKQED"
     gene            24267..24542
                     /locus_tag="ACHLAG_001063"
     CDS             24267..24542
                     /locus_tag="ACHLAG_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654335.1"
                     /translation="MRVLVRIVTSTVYDVFPLFMVKVDGLNDEETDALIQRTLVEYTG
                     HDADSVMVDDDGVCWHNGNCWYVEETQQISNEDAEHLERILSISTFE"
     gene            24758..25093
                     /locus_tag="ACHLAG_001064"
     CDS             24758..25093
                     /locus_tag="ACHLAG_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654336.1"
                     /translation="MKAIPFALLFLSSIVVADTTVYQCEMSVADVKNGALTDVIKAPY
                     GAMVVDSGDQFYVVRDDRVLSSPYLTNRNGKLTGVGEDHFVYNKYKGFYGVHASQQSY
                     LFDDCKEVG"
     gene            25093..25305
                     /locus_tag="ACHLAG_001065"
     CDS             25093..25305
                     /locus_tag="ACHLAG_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654337.1"
                     /translation="MALTLAGLEIEKTSGYWRAKGFKQPGILERLEREDGYIVHQRRE
                     WRMYDPETGKLTTKAGTLWGLLKKIH"
     gene            complement(25794..26849)
                     /gene="repA"
                     /locus_tag="ACHLAG_001066"
     CDS             complement(25794..26849)
                     /gene="repA"
                     /locus_tag="ACHLAG_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HFP0654338.1"
                     /translation="MSENGNKNIAIVEAFSETDKKTGEVVTLVPNTNNTVQPVALMRL
                     GLFVPTLKSTSRGRKGQMVSMDATAELKQLSLAKAEGYEDIRISGLRLDMDNDFKTWV
                     GIIHAFAKHKVVGDTVTLPFVEFVRLCGIPTARSSAKLRKRLDSSLSRIATNTISFRS
                     KGSDEFYVTHLVQTAKYSVKHDTVELKADPKIFELYQFDKKVLLQLRAINELSRKESA
                     QALYTFIESLPPDPAPISLARLRARLNLTSRTITQNATVRKAMEQLREIGYLDYTEVK
                     RGNSVYFVIHYRRPKLRQAQISTKIDNDETEYSLPDENQDDIIDVVPDEKEGKMVMLS
                     KEELALLEELRKAKTRK"
     gene            27593..28237
                     /locus_tag="ACHLAG_001067"
     CDS             27593..28237
                     /locus_tag="ACHLAG_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654339.1"
                     /translation="MDLKRTRWVRRLEDGSYTIESNSNLNKQKLLCDICGIAAKCPIY
                     ETRIKLDKAGVNFHLNSCIRYVPLLAFRKPIIGLDAPYFNTLRSGVTWRDRLSPDKLI
                     CLVSADTGKIIRFGKVDKVYSGPVDEMLRKHSRFNHLCMGGEKIEKVEEVIRKSYGHF
                     LTKDSLLTAIYIRHVKREFDLEYHSEEELNLVDPRPKAGVISINAARKKPTDAL"
     gene            28492..29577
                     /locus_tag="ACHLAG_001068"
     CDS             28492..29577
                     /locus_tag="ACHLAG_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFP0654340.1"
                     /translation="MTLPYGVISDCHYHKWDAFSTTNAEGLNSRLEIQLEATKEAAIA
                     MKKAGCKYMLVAGDTFHVRGTVSPSVLHYVTETYKWIINELDLTVVMLAGNHDLETND
                     SVYSANAAASLSSIGVVIVCGKRPHSIKIGDVTVHLISWRNNHAELISDLKALRKSVE
                     GDNHDVVIHTSINKAIPTMPDVGIDAQELKDIGFRLVLSGHYHNHKEVIPGVISVGAL
                     THQNWGDVGSLAGYMIVNPDGSFSHYETSAPKFINLEDDVADDQIRGNYVRFRAVIEN
                     DEEGIKYQNILKTMGAKGVVCNFIRKSSMMEGTASTTETSKIDSLGESVSAYCKIVHD
                     TDGGFDLSKLDILCQEILTEAESSEAV"
     gene            29807..31723
                     /locus_tag="ACHLAG_001069"
     CDS             29807..31723
                     /locus_tag="ACHLAG_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0654341.1"
                     /translation="MKFLKLQVENFMAIASAEVELDQRGLVLIQGVNSDDSSASSNGS
                     GKSTLMNSLMWCLYGETAHGVKGDDVLSTDHEKNCRVAVTIEDEGKRYAIIRHRKHKE
                     FKNRLIVRGEDGDMTKGKDALTQEFVERLIGASKEVFMASIYASQEAMPDLPGMSDKN
                     LKTIVEEAAGVDRLTRAYAIARERANAAAARMDVVKTKLESTISTIEATQSEIESAKA
                     SSESWEQERSKRYDDALAGLASAEVELTEVELEIRTLPEQIRDTEKAIENERKKLASK
                     EEHDAKLLKVRGAITDIRASIKATENSQADAMNRARNFKTKAEEVGTKVGSPCPTCGK
                     AYCEEDLSTVKESFIEQARQEIGQAKTLAEAMAKHKTNLEKALSIESALVKTTPDVTA
                     IIARIEELTKQLSSLRHREKEVVAIESLVTRARTEVDRISKEINPFIALIARHEDNLV
                     SSKSTFKSLKDELKAIQEQTLLLEKARQVYSPAGVRSHILTSVTPFLNTRTAEYLNTL
                     SDGNITAEWSTMDVTKKGEYRDKFNISVQKKGSSKSFQTLSGGEKRKVRIACSLALQD
                     LVSNRASKNIDLFIGDEIDDALDTAGLERLMGILESKARERGTVLIISHKEMKSWFRE
                     TITLEVKEGRSYVV"
     gene            31713..32456
                     /locus_tag="ACHLAG_001070"
     CDS             31713..32456
                     /locus_tag="ACHLAG_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654342.1"
                     /translation="MSFKLSRSQFLQVFAVMQSIKLINGHTSNGAAPRILWGSNNIDG
                     VQFAALLGLISETPLMQSLKSLPPGCIAPILINPFVEGGYLPNVGPGFIASHETEDLN
                     INSEGFFGGMGAHHCMAFTNLIRLANKRVDSLASPGDAFTGFLIQRRDKKYSADKLQF
                     VGKYGEMVEIELQLPHVLANDSADSRRLLGIMRHFIASGVKHAVDKRVTQENEYSDFA
                     NYPQPTLQTAIVANSLEARLLENPIWGTW"
     gene            32466..33035
                     /locus_tag="ACHLAG_001071"
     CDS             32466..33035
                     /locus_tag="ACHLAG_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654343.1"
                     /translation="MSKKISVVGVDPSMSNFGLAVGTLDLETDELEIHGLTLVETKAG
                     SNKKTVRVNSDDLRRASEIWRVAKPIIDKANMVFCELPVGSQNSRSQTSYGICIGVLA
                     CVDKPLIQVTPNEIKHFVGNKLTTSKEEIIQWATKKHPKAPWLRRKQSGQDVLVNKNE
                     HLADAVAAIHTGMQTDQFRQVRDVLKSLI"
     gene            33109..35424
                     /gene="nrdA"
                     /locus_tag="ACHLAG_001072"
     CDS             33109..35424
                     /gene="nrdA"
                     /locus_tag="ACHLAG_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP0654344.1"
                     /translation="MISIVKRNGQTEPLSEEKYNRVVMYGVEGIRGVSASAVAMGAAA
                     SIFDGITTSQLHEALVKSAADLISPEAPNYSQVAARLNIFKIRKDAFGRYDYPNFYQH
                     IVKNVNKGVYDKDLLTHYSFEEIEELGNYIKPKRDDLFGYAATVQLQSKYLVQNRVTG
                     EIHEGPQHIYMLVGMCLFQNWEDDCAGKTRMEMVKGFYDVTSTFKLSLPTPIMAGVRT
                     PTRQFSSCVLIESGDSLKGISAASAAIIDYVSRRAGIGIGFGRIRALGSEIRNGEATH
                     TGVIPFLKHFQTAVKSCSQGGVRGGAATAFYPIWHLEVESLLVVKNNRGIDENRVRHL
                     DYGVMSNRLMYRRLVRSENITLFSPHDVPDMYEAFFTDQDLFEKLYHKYEADDSIRKK
                     SVPAIELFSSLMQERASTGRIYIANVDHINEHGAFIPALAPVRQSNLCMEITLPTRPL
                     AFTDDPNGEIALCTLSAFNLGAIRSLESLKEVAFYAVAALDSLLDYQDYPMEAAEVPA
                     KARRSLGIGVTNFAYYLAKNGVRYSDTAGNKLVHETFEAIQYYLLDASCRLAEAKGEC
                     DWFEQTKYAIGQLPIDHYRSSLDESGETNFELKMPWEELRERIAKYGLRNSTLTAQMP
                     CETSSQITNSTNGIEPPRGPVSVKSSKDGIVKMVVPEFEKLKEQYEYLWDMPDNRGYL
                     TKVAIIQKFFDQAISANTNYDPSRFEGDKVPMMTLLSDLLLAYKMGVKTLYYHNTRDG
                     AGKRDDDEPQNPLTQAVAVEPEDECDGACKI"
     gene            35531..36673
                     /gene="nrdB"
                     /locus_tag="ACHLAG_001073"
     CDS             35531..36673
                     /gene="nrdB"
                     /locus_tag="ACHLAG_001073"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP0654345.1"
                     /translation="MSYSTFRLGANDATKEPMFLGQSVNVARYDQQKYRDFEKLIERQ
                     LSFFWRPEEVDISSDRIDFNTKLRDHERHIFLSNLRYQTLLDSVQGRSPNATLLPLIS
                     IPELETWVETWSFSETIHSRSYTHIIRGMVDDPSIVFDGIVTDEEIINRAISISAEYD
                     RLYGMTCERQSLGEKEFERLYVNEYGWEPYPLHRQLFRTLVSINALEAIRFYVSFACT
                     FAFGERKLLEGNTKIMRFIARDEALHCEGTERMIRFMRTGREGLLWKEIAADEENVIY
                     DTMKSVAEQEMNWADYLFKDGSMIGLNADILKTYVKYRTNLAMNRLGLKALFPEVTTD
                     PLVWMNKWLLTDTLQIAPQEAEQSTYLVGQIDSTVDKASLSQFADL"
     gene            36751..37620
                     /locus_tag="ACHLAG_001074"
     CDS             36751..37620
                     /locus_tag="ACHLAG_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP pyrophosphohydrolase"
                     /protein_id="HFP0654346.1"
                     /translation="MNFTKLTDHLKLATDRLIGFKPEPYELHEGHGVATESIYKMVDQ
                     FHELFQHPRRVMPTPELLRLRASLIHEEAVVEGIPAAMNGDIEQLLDAMADFLYVGVG
                     TMVAIKGGISTGMTYYTQEQSIDRFMQTIFVPGNTVFDDMAMPFQEAREASCMLEELA
                     DKLENKTVKDSELIQELRRVMNKIYVACMMTYRLADFLGINVVELVGEIHRSNMTKLW
                     PADVEERRQAVANCKYDSSDLGFRHADGTDKMIGFRISDGKILKSPTYSDVDLSSFVE
                     QAKASAMYGMIKK"
     gene            37791..38894
                     /locus_tag="ACHLAG_001075"
     CDS             37791..38894
                     /locus_tag="ACHLAG_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP0654347.1"
                     /translation="MSLLLNREHTNGQVTNASYAKVIETVLKSGVQADDRTGTGTLST
                     CYVPSYYMLTGGTVPLISGKAVNLKPLLVELEWYLKGTGNIQFLKDNGVKIWDAWADE
                     NGDLGPVYGKQWRRWEDTRIVSHSEYLSKIATFRERGYKVEGYLGISEDRVVLSREID
                     QLQRIVDTLRTNPTDRRIMLNAWNVGELEDMKLPPCHFVFSLWSRELDFETRLTMATD
                     IGLQHSRLGYESIYTKMLYDLEMDGSVTEAELDELGIPKRILNSCLVQRSVDTFVGMP
                     FNIAGYGILTHFLAKITGHMAGAFVHFGFDVHLYNNHMEGVCELMKRQAPEHSDPVVI
                     FPHEWSELDDFKWDEVLILGYDPLPWIKVPVAV"
     gene            38896..39309
                     /locus_tag="ACHLAG_001076"
     CDS             38896..39309
                     /locus_tag="ACHLAG_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654348.1"
                     /translation="MARGMYVLCEIEGVLANASHRKSVSDADAGQLIAGDELIFPTSR
                     MLRGFARSGAEVVLISSRSETLEAPTKRWLKDFGVDYDWLHLVPNGTSYEKHIKRTLA
                     EHKGDLLIAALVHDPRLRAALADSHHRPVIYEVSK"
     gene            39306..39782
                     /locus_tag="ACHLAG_001077"
     CDS             39306..39782
                     /locus_tag="ACHLAG_001077"
                     /inference="COORDINATES: protein motif:HMM:NF047324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFP0654349.1"
                     /translation="MKMIAAVGRNYEIGIANELPWRCSTDLKLFKRLTKNATVVMGRK
                     TMESLKRPLPERHNLVLTRSHGFVPNGFYPAGVDDVLRLPEPVWVIGGEQIYSLFMPH
                     VEEIWLSHIGVDVPNADAFFPASMMRNLGFVPVETAYTQRASEEEPGFSQIVYRRS"
     gene            39782..40426
                     /locus_tag="ACHLAG_001078"
     CDS             39782..40426
                     /locus_tag="ACHLAG_001078"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0654350.1"
                     /translation="MDYRIGITGAQGSGKTTLAKYIDKHYGIPYVDAGVGSLMSRLGV
                     RVGESMPLYERLQIQMEIAKHIELLTRGVEGFVIDRTPADVMAYTLDLVGHTNEDRCI
                     ELALDIEKFCHKTAISNFNAIAGLRPGVALSERDYLRSQRASLDRLYVARIDALMCGE
                     LTKIHLHPQRGDLQTFVVSNRYRTVEARARSVMRMLDNAVEKIENRFCGRVTVH"
     gene            40488..40907
                     /locus_tag="ACHLAG_001079"
     CDS             40488..40907
                     /locus_tag="ACHLAG_001079"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654351.1"
                     /translation="MFSEMLLEDELDRKTTEALIRVADEHSRSLMSDREARLAIRAIF
                     ETAQGLVGAQVGEAINIAMSQFSEDSKKPLFPMHLMLAGGTVLYISVCLDSNQINILN
                     TASGKWKDPIVCETSEETLKKAAQFVRSALLKGAKKL"
     gene            40917..41474
                     /locus_tag="ACHLAG_001080"
     CDS             40917..41474
                     /locus_tag="ACHLAG_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFP0654352.1"
                     /translation="MTTIVAGIDIESTGLDFLAGHKIIEIAITRYELETQRHIDSLEM
                     RFNPRRNIDPKAQAVHGISLEQLAAEPLLSNHASEIGAYMGACSAWIAHNGEAFDIPF
                     IRHEFSGYGVRLPDVPVIDTMLSGLWATEDGKRPRLEELAFSLGFIYDHAKAHSALYD
                     TNLMMQCFFKARNKYGFFKLPSEIV"
     gene            41635..42444
                     /locus_tag="ACHLAG_001081"
     CDS             41635..42444
                     /locus_tag="ACHLAG_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654353.1"
                     /translation="MSSVENVMTNDDLDELTAMLQSLDEPVKKAAQVENTDDIDDLLL
                     GLDAGVAMSSDDVAEELFNEEKAGDFSSALNELELAHEPINVINAESVESAENEPEQL
                     GLIEVEECVDVNDELKVQQSNDSNTNKKARTARGPRFTLSDKDDSFFNKAGLETDIFL
                     DAYENAPVKAKDKILNLLNWFSGGPDISVYTVISMRHLLTEKKATSNSIKIALMSNPE
                     KPYPLNTASTQAGQMMAVFPATGIAVRDGGNLTLNEESPIVKKFVAEYTIG"
     gene            42628..43221
                     /locus_tag="ACHLAG_001082"
     CDS             42628..43221
                     /locus_tag="ACHLAG_001082"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFP0654354.1"
                     /translation="MIAAEKIKQRKRDNSLRDLWRTPDWLFSAIQRYLGVTFDVDVAC
                     NKDNAKLPNFIGVERDALKSEWGQPGTIAFLNPPYSKISPWIDAAIREQARGVTTVML
                     IPQSLDTKWYERATECANETIILSGGRVAFVEPDVNLGQVEVNINPGGSMLVVFRGFC
                     QDAGHSISKIPLDVMKSLGGYDPANVIRKKRQSKKAA"
     gene            43408..43638
                     /locus_tag="ACHLAG_001083"
     CDS             43408..43638
                     /locus_tag="ACHLAG_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654355.1"
                     /translation="MSYPTNVVALVESDFLAQAREMMKDREQAFNLYEWAIKCLHLGE
                     HRELVEQLLGELINEVFALNVQLHGRKNNQSK"
     gene            44155..44815
                     /gene="rnhA"
                     /locus_tag="ACHLAG_001084"
                     /pseudo
     CDS             44155..44815
                     /gene="rnhA"
                     /locus_tag="ACHLAG_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
BASE COUNT        12708 a         9847 c        10822 g        11529 t
ORIGIN      
        1 cttattaaat atatacggaa gcaggtcttt taaaagacac caccagaaca actcccttcc
       61 gtttccactt ccaaaaactg ccaccagtcg ctatcatccg ctcattgtga taagtaagta
      121 actacctacc aggtgagcca catgagccaa atctttttcg ataccatcga caacgaccag
      181 tacgacttca tgacagagtg gaataccgct gttatggaca agtgggtcgc tgaaaacatt
      241 ggtttgtcgc gctgtaaaga cgaggctgaa ctcttcgaga cgaagtggtt tgattaccgc
      301 gacatgcatc ctctcatggc cacctgtctt tttacggagg catacaaacg tcagtactca
      361 aatatcatgc tgacgcacgg tcgcgaacac tttgaaacag ctccgttcac caccgggtta
      421 aaacgtctgc cttatcagga gttgtcgact gccaacaaaa cgtcgctatg gaaagcacgc
      481 cagtttgctg accgctattg ctgctcatac gactacttta tctccaccgt tctttccgca
      541 gctgcacgac gactgtggga caagctgccg cgtccgcagc atttgtggca gccagaactg
      601 attgagatat ttgaagataa attagccaga cgcgcaacaa cccgtctgga tgactctctg
      661 gttagcttta agcatatggg agacatgcag ttcaacccga ttcaggaaag ctattttgag
      721 tggattctgg agcgtttgcg caccatcccc cgcagcaagc gcatacgcgc aattttctcc
      781 gctatctggc taatggaaat cgttccagag cgccttattt ccgcccactt tccagaagaa
      841 ctggaagaag cacggcggtt tattgatccc ctatctaatt aactaatact agaaaacaat
      901 ttgtttaaaa aacaaaggaa agcacatgac cgaactttgc catacaggac gcgggctgtc
      961 cgaagagttt gatgaagatt ttcagaacag attgacggcc tatttttgtc gtgatcacga
     1021 gtttcttact cgtgctggag atctggttgt acctagccaa tttgccaatg cggccaatgc
     1081 catattggtt aatatggttt cgggctatta ccgtatgtac aagagcgcgc cctcttcatc
     1141 tgcgattctg gatatgctta agcgtgcgaa acgcgataag actatccgtg aggaactatt
     1201 cgccgatgtt gttgctgcgt ttaagcgcat ccttgcagaa aaattgtctg atacctcgta
     1261 catggttgac caggtatcaa ccttcgcaaa aagcgtagcg tttgatgatg ctctgattaa
     1321 ggctgctgaa ctgaaagaga agggcgactt tcagggggcg atggcaatca tggccaaggt
     1381 tcagcagatt ggatcgaacg aagcgaccgg aatctatgac tactacacct ccgcaagtga
     1441 gcgattgaaa gcgcgtgaat atgaggcttc agaggagtat gtgccaaaca gcattacaac
     1501 tggactccct ctgctcgata ggttgctgta ccaaaaaggc tgggcgaagc gtgaaatggt
     1561 gctcttcatg gggttcgcta aatccggtaa atcgaccgca atgggtgagt tttccataaa
     1621 cgcaacgctt gctggctaca atgttctgta tctctcgctg gaggttcaca ccaccatttt
     1681 atccgaccgt tttgatgcaa gattgtcgga gacagaaatg tccaagctgg tggaacggcg
     1741 cgatgaggtt catcgtaagt tggcagagtt gggagccacg aaggggattg gtagtttgtg
     1801 ggtggttgag cgtccgtcag gaagtatgtc accggcagat ctggaccgta tgcttaacag
     1861 catgaaagcc aacggcatgg tacctgacat ggttgttgtc gactacgcag atttgatgcg
     1921 tgccagttat gaccttcgtg atgatcgcgc caacattcgt agtatctaca ccgatttacg
     1981 tgctctttat gacaagcata acgttgctgg gatcacggca tcgcagacaa accgtgaagg
     2041 tggcgcgtca gaagttgcca caatgatgca cgctgccgac aacatcgaaa aagtacgtat
     2101 tgctgacctg gtaataacga tcaacaaaac agaagaagaa gaagcgaaag gagaggctcg
     2161 tctctacttt gctggttcac gtaaccagca gggagggatc agcattcgcg ttaaacaaaa
     2221 cctcgaacaa atgcgcttca ttgagcgaat cttagacgtt acctaaaaaa taagcgtgga
     2281 gaacgcctcc acgcttgatt cattggtgaa acaacttttc ttttgccaaa ccacaaaaga
     2341 aaaacacatg agcctttatg ttatatcaac atttaggttg gtcacaatat tgcctattaa
     2401 aagtggaatt atcgtgagcg agctgaaaga gctaattacc gaattagatt ttgaacaatg
     2461 gttggacact gaaggtatcg tttatcgacg tggaggcgtg agtgctcgcg gtcgtgaagt
     2521 gaatatcaag gagtgtccgg tatgcggcag ctccaactgg aaggtatatt tcaatctgac
     2581 cagtggcgtc ggtaaatgct tcgctggtga tcatcccgaa gagattcagt tcaataagct
     2641 ggtcttcctc aagcactaca gcggcaaatc acgacgacag ttcgaggaat atgtgcagaa
     2701 cgcccttctt tcccaggggt gggcaccaaa gaaagaagag atagtgcttg caagcacagt
     2761 cgagttagag ggaccacttg cactccctcg tcattacgaa cttcctatag atggccgtct
     2821 tccagactat ctggttgaac gaaacatatc gcctgaaatg gcaaagtatt ttgacctacg
     2881 atactgcgtc gaaggtaagc acgcctatgt agatccgtat acagatcagg ttaaaggaca
     2941 gatattcgat atgcgaatac tgataccggt ttacgatctg gatggggtaa tgaagacatt
     3001 tcagggacga gacattaccg gtacagcaga acgccgctac ctctttccta tgcagcttcc
     3061 agcttcaggt aagtttctct acaacggcca taatgcagtc ggcaaacaga ctgtagttgt
     3121 ctgtgagggg gcgttcgatg ttatgggggt taaacgagct atttttgacg aagaaacatt
     3181 acgtgattac gtggaaccaa taggaacgtt cgggatgcat ctatctggta acaccactca
     3241 ggatgcagaa gatcagttag gcgcgttcct gacgctcaag gcgcgtgggt tacgtaatgt
     3301 gatcatgatg tgggatagtg aaaagcaagc tatacgtaat acgatggccg cagccaggcg
     3361 actgaccagt attggtctta atgtcaaagt ggcgtgtttg ggcgaggaag gactcgaccc
     3421 gggcgatgca acgccagagc aaatcatcaa agcctattat cgggcaaaac cgtataccaa
     3481 gcagttggag ttacaaagca aggttttggg cattaaggct ctatgataac aatcaccttt
     3541 aaaataagta gatgattact tatctttctg tgagaatact ttcatctgtt agctaggagt
     3601 tggtatgaaa gacgaaattc agaaattagc ctgcgacatc attgataaaa ctggtttaga
     3661 aatcagcgag agcaatcggc tagacatcat tgaaaaagcc gtaaaaacag caatggatca
     3721 tatcgccact cgtttggtcg agatcccgct accagggcta ccttatctga aagttgagtt
     3781 acacgtatgg ggtgaatctt cttgttcacg gcgttctgca ttagttgttt ttattagcaa
     3841 agaaaacccg ctcagtctta aagtgcaggt tggtgcatgg atggatggaa aagtgatcta
     3901 cacaaatacc gttttttgtc tttcaaacga cgaaactatt gaagcggcca ttcaagaagc
     3961 agttctagca atgcgcagca gcggtttgat gaagaataac tacgaagagt acttgcgttc
     4021 gataagtggt gaaaagacat tatctctgaa agcagatttc gttaccccga aaaatctgtt
     4081 ggaagtcttg cttaataaag gggctaatga tgccgtaaat gtaatcagag agagtgagta
     4141 tgcgtctctt tgcgacatgt gcaaaagcca gttggatctg gtgcatatcg ttattgatgc
     4201 tgggaaggca tgtgatggcg taatggcgga atttgcttgg aaggtggtca ggattgctaa
     4261 cgaattaccg atgatagagc aagaggccaa atcatacgcc accaatcatg tcacagagct
     4321 tcttgtcccc tatcgcttag aaagcaatca gcgcaagatg gttagctggg gaagttggta
     4381 atctctccgc gcgtcgtttt ttacgcaaat aacgataggt aagtacaaga ttatttatgg
     4441 cggtagttgt gaaagctgat ttgtcaaaaa tcccttctat ttcaggaaat aatggttatt
     4501 cacttcgttg tgaggaagta aagataaacg gtgagtcggc atattgcagc tactccgttt
     4561 gccagcacac cattcttgcc ttcaaagaaa accgtcttcc tcgaacttca ttccagtcgt
     4621 gcgcaaccgc tatcaaagca ggcaaatgca aggcgttaaa aatgatggtt gaagagattc
     4681 gtaaaggaga atctctgtat ttcgaagata tgaccgcgct cattaaggag gttgaagaac
     4741 ggaataaaca agccagaact ataaaacgaa aacgtgacag tgtaacgatt aatagcatgg
     4801 ttaagaagag caccacatca caaacagcga tcactgacgt gtatgcggcg ttgcttgaag
     4861 aaacaacaaa agaaacacat gagcaaatcg atcaacatat ggaggcaaaa caacaatgaa
     4921 aaagttgatc gcacttaagc ataagctgga cgaaatgaaa gctatgggaa ccaatgcaaa
     4981 aaaagaggca ttggccaaca tggatgactt cgagcaaagc atggtttcat tgatgctcaa
     5041 ccctttcatc cgttttgggg taaagaaata caaagtggca gagccgctta gtgagtccat
     5101 cccaagtgac gaaaaagcca ttgatgtact gaataagctg gcctctcgcg agctaacggg
     5161 gaacgcagca atagctgctg ttgagtctat cgtggcgtca atgtgcgccg atgggcagga
     5221 cgtgttccgt cgtttcctct taaaagaccc gaaagcgggt gttgggatta gcctatgcaa
     5281 caaggttttt gaaaatccca ttccgaaatt tgaggtgcag ctggcgtcac cgtataaaga
     5341 aaaaggcgac aaatacccct tcaagccaaa tcctaaagca aaatggccga tgattggcag
     5401 ccttaagctc gatggtttgc gagtaatttg cgaggttatt gttgacgagg aagaggtgaa
     5461 cttcctttct cgtactggta atccaatcac gtctctcgat cacctaaagc cagccatgct
     5521 cgaattaggc aaactttcag gccacaaaca catcttcttc gatggtgaag gaacagccgg
     5581 ttcatttaac cagtccgtat ctgcattgcg caaaaagaac gtgcaggcaa ttggcgctat
     5641 ttatcatgtt ttcgacttct tcctaccgga atggcgggca caggctaaat ccaaagagta
     5701 tgcaaagaca ggtatgaagc tgaaagagcg cctggctatt ctcgtggcgt tgttcaaaaa
     5761 cgatcgcagt gaagactacg cgcaagacat tcacctacat ccgttctaca tcatccatag
     5821 ccacgaagac ttcatcgaac gcttcatgaa acgcctggac gataacgaag aaggggagat
     5881 gggcaaagat ccgaactctg tttacgagtt taaacgtacc cgcagctggt ggaagttaaa
     5941 agacgaagat tcagaagatg gtgaaattat cgattttgag ccgggcgacc cggactctgg
     6001 ttttgccaac acgcttggaa aaattgttat tcgtcttgaa aacggcgtca tcgttcgtgc
     6061 gagtggcatt aagcataaat atctggacga gatctggaac aacaaagaga agtaccgtgg
     6121 tcgtattgtc gaggttcatt gtcacgagaa aacacctgat ggcagcttac gccacccacg
     6181 actgaaatgg ccgcgttgct tacgcgatac cgaagatcga atcggagata aagaatgatc
     6241 gtattaagta aaagggagaa agaaacgctt catgaaatca gtaagtggcc ggagttccct
     6301 gagtactgga agcctaaaac gcgagctaag ttagagcgtt tagggttggt tgcaaacgtt
     6361 tctgaaacgt ggtgctcggc caactaccag ttaactgata aagggaaagt attgctacag
     6421 caattagtag aatcaggagc gttaaaatga ttccatacat ctcattagct tttatgggtg
     6481 gcttccttat tggcttcggc atctgtcgtg atttaattaa gcaggaactt aaaaccaaaa
     6541 cactgtgcat cggaaagcgt gtgtatcggg tagttcatga aacaaaggtt agaaaatgag
     6601 caatttaact tcttgggact ggtggttggc cacctatttc ttagcggccg gagtcgcatt
     6661 cgccttttac gtaggtcagt tagtcgtaaa actgttgctg ataaaatttg ctagtcataa
     6721 acgtatcgat gatggtctgt ggcgtcttgg caccctggtg gaaactcgct acgggcaact
     6781 taaggagaac gaaaccatta ctatccaagc gaaacggttc actgccacca tcacaagaac
     6841 acctagtcgt agagtggcct tgatcaaaaa agtcacaacc gaataaaaat acaatgataa
     6901 gtatttactt acttatcttt tatgtataag atgactttgt tttcgttgag acgcgactgt
     6961 ttgaacttaa atacaagtgc aaacgaagaa gtctatctgg cagtagccta ataagccaaa
     7021 caccagcgag gtcagtttcc agcctcgtta ccgaaatggg acacactgag cgagtgtgat
     7081 tgcagaacgc aggagggaac attcatgttc cctccgatga agtaacagaa tgggcggttg
     7141 gtatattttc aactccatat gactcccgga ttcttagcca ctgaccgccc gtcctgttac
     7201 gtcattttgt tcaattatgt cgtttatact gggttaaaaa gcggcgacgt agcccggctg
     7261 gtatggttag ccagcacaca acgttgaggc cattacattt ttatcaattc taaggttcta
     7321 ttcacagaga taccggcgag cgttgatatg taacatgttg ggcaaacatt caatcggagt
     7381 agtggcctca acgttgtgaa gacaggattg ttgtgtaggt ttaaccactg ttgccattgg
     7441 tgcctgtttt cacaacaaat gattccatac atcacattgt ataaattaca aagtaggtgc
     7501 tgtcctcaga aacatcatct acttaaagat tttgccttct actattgagc gaagtcgaaa
     7561 gcgtctggca ctaacgaaaa gtgcaagtag cggtgcgttt cctggcagaa actaaaccgt
     7621 cgcgattggc actgttgagt aataaatact ggcagtgctg aattgatggt gtagctcagc
     7681 ggtagagcag ttggctgtta accaactggt cggtggttcg aatccaccca ccatcgccaa
     7741 tttaggggag ttagtccgta ggggcagcgg ggtagactgt aaatctactg tcattgcgac
     7801 tcgggtggtt cgactccatc actccccacc aaattgccgg tttagctcag ttggtagagc
     7861 gcctgccttg taagcaggat gtcagcggtt cgatcccgtt aaccggcacc aacacaacag
     7921 gtaagagcat tgggcgaatc ggcgatactg acccacaagc ccgtaaatcg atagagtcag
     7981 accagtgctc ttaccgttgt gaggaagtgc agctctttga agcaaccaga agataagcat
     8041 ctggcttcac aacacaacga taagatcatt acggttaatc gtcgttcatg tgcacaatga
     8101 ctggtcgaaa ggtagtgatc ttaccgttgt gatgaatgca caggctgatg tgccgcaact
     8161 acagtagtgc gcgctttgcg gggcttgcta caaccctgtg tcggagttca gcaccgacca
     8221 tcacaatagc tggagagtag ggagcatggt gctcaagcgg tcttgaaatc cgtcccattg
     8281 tgcaaacgat gatggttcga ttccattact ctccgccaga cacagcgttg agcggtttgg
     8341 ccttttaatc acccagatta agactccgct aacataaacc agaccgctca acgctgtgat
     8401 agacaattac ggcagacgtt cttaaccata gcttgctaac atcctagcaa cacttttttt
     8461 cagcgcaaaa ttcaaagggg cttcggcccc ttttttgcaa ctgaaagagt caagtctacg
     8521 aactttgtag gaagaaataa tgaccagcaa agagaacaca ccactaaaag tcacccctga
     8581 acacattgaa caactcattc aaagtgaaca ttatttcact gcatatgatg ccagccacgg
     8641 ggataacttc atctctgttt acaattcaaa aacagatatc gacaaaggtc atgagtcgtt
     8701 aaaactctta acgttctgcg ttatggttct caaaaatgga tataccgtaa cgggaaaatc
     8761 tgcgtgcgta agaccagaaa tcttcaattt tgatgttggt cgcgagtacg ctcgaaaaga
     8821 tgctatcgat caaatctggc ccctggaagg ctatctcctg aagcaaaagt ggcatgaggc
     8881 aaagcaatga cgatcactat ctacggacga gataactgct catactgcaa acgtgcggtc
     8941 gagctggcga agcaactaaa gggacatggc tacggtgatt atgagtacat cgacatcacc
     9001 actgccggta tcgacaagga aaaactaagt gaaattgttg gtaaaccggt agagactatc
     9061 ccccaagtgc tgatcgatgg ccaaccgatt ggcggataca cagaactggc tgcatacgtc
     9121 agcaccctct gattttaacg gctcacagga gccgttttta ttcccaccaa actcactccc
     9181 gtttccctta aaattcaaaa aacaacgtca taatgattcc atacctacta tgtatggaat
     9241 cattactgaa aatgagttac ttttactctt gatcctataa gaatctatgc ctaatatact
     9301 gtttacttat acagtgaatc ggcgtaactc ggtgattgtc atatgaaaaa tagctttgac
     9361 agagcacgcg ctgcggagaa cacctcaaaa gaggcgattg agtatctcga aagagcatct
     9421 caaatgcagg ccgttatgat ctcgcaggtc agcaatgaca tgagattctc ggacgcattc
     9481 atgttattca ctcgcttatc tctgctgata acaagacgtc ggccagagat cgctgttcat
     9541 tgtattttga tacatgtttt gccgcacatt gccgatgtaa aagtaagtga cattaatagg
     9601 ttcatggtga accaactggt caacccacta atactggatg gcaaaattgt tatgggccgc
     9661 cgcgttttct ctctgatgaa gcagttcctt agctggtgcg ccttccaggg gatgatagac
     9721 gtgtcaccgt taaacgatat gtcactaaac aaagttgccg gtggcgcaaa gcccacacct
     9781 cgcgagcgga agctgaccga cgcagaggta tgggtgttct ggaatatatg ggactacttc
     9841 aatgtgtgcg ctggtacaaa atgggcggcc aggctgtgtc ttgtatccgc aagacgacct
     9901 gacgaagtac tgcgggctaa aaaaagtgag ttcaatctta agcgtggggt ttggaatcaa
     9961 ggcaagagga acaaatctgc ccgtgagcat tcgctgcctt taagcacatt aatgcgcaca
    10021 tgtattgaag agttgttcga atacggtaaa ggcagccagt ggctcgtgcc ttcgaataaa
    10081 aaaatcggga aagaccttcc tatgtctaaa gtggcaatag cccaggcatt acgtcgtatc
    10141 ctggaacgac cagaactgat ggagcttgag ccatttacac cccgagactt gcgccgtact
    10201 gcgcgtagtt acttcccagc attaggcata agccaggagg tatcacgcaa aatcatgaac
    10261 cacagtcttg aggggataga tcgagtctac gaccggcacg attatatgga cgagatgcga
    10321 gacgccttag aaagtttctc gacgtacatc gcatcaatcg tagagcaacc ggatttagac
    10381 gaaattgacc acaaattcaa gggagatcgt ctatcaacag agcttattcg tgtaaatttt
    10441 tcatagagac tttatggcct caacaacctt ttgtgatgcg cctttctctt taccgaatcg
    10501 ctcgttatat gcagcaagaa cctgtttttc gtcctcgtta agaggagcgg tgccttcttt
    10561 gtataaaaat gctgcgagtt caggttggcg ttcttccagc accatcatca taagacgact
    10621 tggctcaata cccagcgcca gcgccaacgg acgaacctta tcgataggca aaggaatttt
    10681 gccactttta attaaagaaa ggttgttggc atttttataa ccaataagtc tggctatttg
    10741 ggcctgactc ataggtgagg attcaatcag ccctgcgata aaagcagcat agcgactttc
    10801 tataaattca atcttgttgt cagacattgt tacaaccttt gcgcgttcaa ttctctctgg
    10861 taagtgctta ccgatattac atcaaaggtt agggttgtaa agctattatc atttttttcg
    10921 ataggcactt aaaagaccgg ttaaaggcca ttgcacggag aaaaattagc ccaaaacagg
    10981 taagaaaatt aacttgtata tgatatgaat gtattcagta ttgatataaa ttttagtagt
    11041 attccttacc atagtataag ttagaatgga ttgattgaat gaacaccact atttccagcc
    11101 taatcgctct tgagatcgga cacgtacaga aattagctga tgagtgtgta gctgacatcc
    11161 tcaccgatct accgaatgag cagattcagg ttggtgtgaa tgacacaact ggctttatat
    11221 tcgaatttaa caacaaacgc ttcacgcttc tcaataccgg ctccgggtct ttagccgtca
    11281 gaatctgtta acccctcttc tccctgcgcg aatggcttag ttccctgttc gcgcagtgct
    11341 acattaaaca cactagtaaa taatttgttt tcataacaaa ggattagcca tgtctaaaaa
    11401 acgttccatc aaagaggttc aggacttccg tgacagtgta aaacgagtag tcgctctcct
    11461 ttcaggtaaa aacatccctg ttgcagaacg aggggacgac gcttatgttc gctataacga
    11521 tgatggagag ccaattctcg taaacatccc atcaatcccg gataacgcaa caccggcatt
    11581 gatgaatgct gtgcgcggat ttctcgatca tgaagttgct cacattttgt ttaccgatat
    11641 tcgtgtgtcc aacaaaatga gagaaaaagg acgcgttcct tcctggtcgc tatggaatgc
    11701 cttagaagac gtgttcatcg agcgaaaaat gggtgaggtc tttaacggaa caagacgtaa
    11761 tctgatggca actcagcgcc ttataatcga aaaagtcttt aaaccaaagg cttcagaggc
    11821 tattgcttat tgtgacaaag atcagcgcgc gctttttcta aacttctttc tctgtccggt
    11881 tgtaagagcc tgggatggcc aagcaccgtt cgtagatttc atggatgaat attggcctgt
    11941 cattgagaaa ccaatttcat tattaaaaga acatggtatc gatgtggccg tgcgtaacat
    12001 gtcttgcacc gaggattgtg taaaggtggc tgcgaccata gctaagatcc tcaaagacac
    12061 tgaaagtgaa agcaaaggta aggagtcagc tccgggaaaa acttccgatc cttcagacgc
    12121 tgaccagacg gatgcctctg gagaaaacag tgaagacaac gaagatcatg agacaccctc
    12181 agtgttagat aatcacaaat ctatcaaatc agaatcacat agtaagcaca aacatgataa
    12241 taacgacggt gatgattcag ataattctga atcatcagaa acaatattcg atgatacaga
    12301 aaatgataaa gaggtatcag attctgatgc ttctgataac gcggcgtcag aatcattaac
    12361 cgctgaccac gaaaaaagaa aaacgacaga agacggctct tcagatatcc caactccgtc
    12421 aaaaatgagt ctggaagagg ctttagagga gttggatagc atggaagatg aagtcggagg
    12481 catgacagaa gatgcgctat ccgaaacgat taaaagcgag ttaacagaaa gctcgaaaag
    12541 cgaatacagg ccatacaatc gctcatacga cttcatcggc tcgattgatc aggcagaagc
    12601 ccatatcaaa cggcttatta aaacattctc cgatattgat ttgggaggat atccaatcag
    12661 ccgctatcgc atcgttcctg aaggcaacca gctcttcgac aaatatattg aaaagcatct
    12721 ttcgtcaggt gtttcgtcga cgctggcaaa agacctggag cgagcaatag caagcagaaa
    12781 cagagttcag tttataccgg gccagcgtcg ggggcgcatt catggttcta gtatctacag
    12841 attagcaatg aatgatgctc gcgtgtttcg taaaaaagaa gaatctaaag ccgttaacgc
    12901 ctgtgttcaa caagtgattg atttatcagg ttcaatgagt ggtataacga ttaaactggc
    12961 tcttgcaagt gcatatacca tcgccgatgc ccttgatcga ataaatgttc ccaacattat
    13021 caccggcttc actacatttg gtagtcatat ggcggcagga gaacttaagg ctgtcaagta
    13081 tgagttctct cgctttgaat ctttaatgct acctatcatc aaaaattgga atgaaaaggt
    13141 aaattctcgc gaagttcgct cacgtatggg gtgcgtaggg tacacattcc cacttcttaa
    13201 taacgtggat ggtgaaagca tagccagcct tgcatcgtta ttttccggtc gcatggagga
    13261 caggaagatc atgcttgttc tgagtgatgg cgcgccgtgg gctgttggga gaggttttga
    13321 cgctcatttg cgttcagttg cgaagcaaat tgaaacgcag actgatattg atttgatggc
    13381 aattggcatc atgactgacg caccggagag attttactca aatcatgccc tggtaacgag
    13441 cgttgatagt cttggttcat ctgtagttac tgaactatct cgtatcattt taaagtgaac
    13501 aaaacagcct taatgataag taaccactta cgatagatga tgatatattt atataagaag
    13561 ttgaacgctc attagagaac aaaggaaaaa cgcatgacga ctactgcact gcaaaatgaa
    13621 aaaaatcctt ctgattacct tgtttgcaag tggtgcggca aatcatttca ctattttaag
    13681 tcccatgtag ccaatggtaa ttgcgagggc attcctgagt cagtaaaaga tgccgatcct
    13741 gacaccgtac tgaaaatgta cacaacgcag tttccagatg aaccaacgct atcgaaaaag
    13801 gcacttgatg caattcaagc taaacgtgcc gagcaaaaaa gcgaaatggc caaatcttct
    13861 ggcttgacca gtagccctgg ctacacaggc acagttgagt acaagacaga tctggtcgca
    13921 gctcacgaac tgctaaacgt aacggtggaa gaactcggaa caaaacgtgg gacgccgctc
    13981 atggttagcg tcaacgtcaa tacgccgtat ccagagttcg ttcccgaagt gaagaaaggc
    14041 tacgtatatg gcgacttcga actgatcaaa gatattttca tgatgcttga acttggcata
    14101 cctggctatt tgtggggtca tgcaggaaca ggcaaatcgt cattgcctac acagctatgt
    14161 gctttgctca atcgtccgtt gatccgtgcc caacatacag catcaatgga agaggcacat
    14221 gttacggggc aaattctggc gcgtgatggc tctacgtatt tcgaacctgg cttgcttgcg
    14281 ctcgcaatga agcatggctg ggtttacctc gcggatgaat acgactttgc gtttccacaa
    14341 attcttggcg tgtatcagcc agtgctggaa ggtgaagcgt tggtcatcaa agaggcaact
    14401 ccagaatggc gtcgcattac tccgcatgaa cggtttgctt tcattggcac tggcaacacg
    14461 aacggatctg gtgatgaaac cggcttgtac cagggtacaa acatccagaa cgccgcgaac
    14521 ttttcgcgtt ttggcatcgt ttcgaatgtg aaatacatga gcaaagacgc agagatcaac
    14581 atgctgacaa atgcaggcat cgtggatgaa tacgctgaaa agatggttaa gtttgccggt
    14641 atcgttcgcg atggatacga agaacacctc attagtcagc caattggccc tcgtgaactt
    14701 ttgttgtcgg ccaagattgg aatgatgcga ggcgactttg tgacaggtat tgagcgttct
    14761 ttcattaaca aactcccttc agcttctgca caagcggctc gtgaagttgt tcaaaaaata
    14821 tttggttaat cgtgcgtaaa ggatgtttcg gctctcttat cgctgcttct gaaactggta
    14881 aggcttgtct ggtgtgtcca gacaagcccg attgtcacca atcagcaaaa gaagttgcga
    14941 tttcgatgca tgggaagttc gtaggcttcc ccaatgacaa aatcaaaaaa accagaaagg
    15001 taaaaacaca tgaaggcact gatggttcga actgacttct cacttgggga gtcggctcta
    15061 aaagcagaaa acgcggtgaa gattgccaga gaagctggct acaccgctgt aatttcagca
    15121 gatagcatga atattgcgag cgttattcca ctacaacgtg ccgctggtga cgacatggcg
    15181 gttatttgtg gtgtgaaact aaacattgtt gatgatccca catacgagca ccgggctaaa
    15241 cttgctaaag aatctatgag atgtatggaa tcattagagc gaggacgtaa ctactcgttt
    15301 accgctctaa ttaaaaatga gaaaggatat cgcgacatct gcgaactaat gacggcggcc
    15361 aacacacgag aacagttcta ctttgtaccg cgtctctcgc tcgaacagtt ggtttctaca
    15421 tatgccaaag gcaacatcat cctgcttact tccgacatcg gtagcgtgtt ccaacgcaac
    15481 gattttgcaa aaatcataag cacactgatt acagcgggtg gaaaagacaa cttctatagt
    15541 gtggtttatc cgcaccctac cccattctac gaccagatta acgtccgggc gatgaaagtc
    15601 gccagcgcat tgaaaataga gccagtggcg ttctatcccg cttattacga atcgatcgac
    15661 gatgcagaca ttaaagacat tgcgcacatg gttacgaaca acatcaaaat cgaccagccg
    15721 catcgtctgc gtatccccca ccagcgagat aacgccgtca atggtcgccg ccatctcctt
    15781 gaggcgctta aagccttctc cgttcgcatg gatgtgccgg taacagctgc aatggcctca
    15841 acaacgcagg ataccattat cgatgcctgc acatggcgct ggcatgaatt gccgccagca
    15901 ctgccaaaga tggcagacga cgagcctgca acgctgatga aactggctgt tgcagggctg
    15961 cgtaaacgtc ttaccacaaa agagtttggc tacacaccac ctgcttctga gaacagggtt
    16021 tatgttgagc ggctaaagta cgaaatggac acgctgactc gcctgggatt ctgtggttac
    16081 ttcctgatgg tgcgcgatct gatgaatcat agtcgtgaaa ctggcattcc cgttgggcct
    16141 ggtcgtggtt cctctgccgg ttctctggtg gcgtggtgca taggcataac caacgtcgac
    16201 ccaatccgtc acggtcttct gtttgagcgt ttcatcaacc ctgagcgtct cgacttgccg
    16261 gatgcggact tggacttcag ccaggcacgt cgccatgagg tgatcgagta tctgaatgaa
    16321 cgctacggcg aagattacgt tgcaggcatt ccgaacttca cctacctggg cgcagcctct
    16381 gcactacgtg ataccgctcg tatttatggt gtggagtccg cagatatggc ggtatcaaaa
    16441 gagctgaaga acgtcgagga tgatagcctt ccattggagg agctgcgcga acaactggca
    16501 agtctcgaca aatacgcaac aaaatatcct gatgcattca atgcagcctg caagttacaa
    16561 agccttatgc gtggctttgg tagacatgcg gcagggatga tcgtagcagg tgttcctctg
    16621 acagaacgta caccggttga acgccgtggt gacgcgcgtt gtatcgcatt tgacaagcgt
    16681 tactgcgagg ctatgggcct aattaagctg gacgtacttg gtctggcaac tctcgatttg
    16741 ctcgatagtg caaaacgcta cataaaagag aacacaggtg aagatatcaa tcttgatgcc
    16801 atttctcttg aagatcgcaa ggtgctggat ggttttgctg ctgggtacac tcaaggtgtt
    16861 ttccagcttg aatcaggccc aatgcgcaag ctgcttaaag atttaggtgg tggaattgag
    16921 ccaatgagct ttaaaacggt cgtcgctaca actgcgctct tccgacctgg cccgattcaa
    16981 tccggcatgt tggacgatta tgtgtccgtg gccaaaggct tcatggctcc acattcaatt
    17041 catccgcgtc ttgaggaagt cacccgggag actaatggtg ttttgctcta tcaggaacaa
    17101 atcatgcaaa gttcccgagt acttgccggg ttctctatgg ccgaagcaga cgctctgcgt
    17161 tccgctatcg gtaaaaagaa catggataag atgaaagcga tcggcagcga ttttgtagaa
    17221 cgagcacaag caggctgggt gacactgtca ctcaaaaatg gaggtactgt agaggttcac
    17281 aaacacgcaa aactggattg ctcagacggt aagcgtagaa gctacagcga ggctattagt
    17341 gacggcatag actacgtgga gatcgtctct gaacaagaag gtctcagtaa ggaaaaagcc
    17401 caagaaatat gggacgcctt tgagaagttc ggtggatatg ccttcaacaa atcacactct
    17461 gttgcttact ccttaatcag ttatcaatct atgtggttga aaactcatta tcccgcagag
    17521 tttttcgctg ctgctctcac tattcttggc gaagataaac accaggggct ggttaaagat
    17581 gcgctgacct atggcattcg cgtattgcca ccagacgtta acgtgtcatc taaccgaatc
    17641 gagatccgca cgcttgaaga tggcagccag gtgctgtatg cgcccttctc tgctgtgaag
    17701 gggtgttctg agaatggctg ccaagccatc atgagagcgc gagaaaaagt tggcggcaaa
    17761 ttcgagtcac tggcgcaatt cgaagaagct gtcgagaaac gtgcctgtaa tagtcgagtg
    17821 cgcgaatcgc tgcaaaaagt aggggcgttt gcatccatcg agccaggcag tctgccagca
    17881 actgatccag agcgcctacg cgaccaggct gaattgatgg gaaaccttgt catagacgca
    17941 gttaaagcct cacgtccgtt tgagatgaac cccaaacgtt cggctgagat taacgtactc
    18001 atgacgcgga tggcggctga aatgggctta ggtgatgaac taatccgccc cagcattggt
    18061 attaagccga aaatcatgat cattctggac aatgcgaacg gcaatgatgc tcgaaccggt
    18121 tactttatgg agaacggata cgacgacttt aaggccaagc tattgacggc tggagattta
    18181 cgcatgggcg atctctatgt cacaggtgtt tgcaagaagg ttaaagacaa agaaaaagac
    18241 tacaccaaag acgagatcgg ccagttcacc gactttatgc gtgaagagat caatctggtg
    18301 cgcccgacct atgtgctgac gtgtggtagc cgggcaacgg cattgtttaa caacaaaaac
    18361 aagccatccg atttggtcgg caggaaggag tatcttccag accttgacgt tactgtcttt
    18421 tacgggttta accccaacat tctttacttc agacctgaag agggagaacg actagaagcg
    18481 atattagccg acgtagcaga aaccattaag acgtgatccc ataaacatgg cctctatggc
    18541 catgttttct cttatcccct catatcccct atcctcgaac tccttgcctt atcatcacaa
    18601 tgatataatc aatataagat gataagtaaa aaggaaagca catgatcacc gatatttacg
    18661 agaaaataat gtctgatctc gagtttgacc gagacaatct ggaggaagtc tggcgtagac
    18721 aaccccgcct tttaatggag tatggctcaa aactcgctca tgcagaaaga agtgtcgcag
    18781 aggcaaaact taaccttgaa gctgttgaag caaagctata cgacacagag cgtaagaact
    18841 tgagtatgaa cggcattaag ttcaacgaat ctgtactgga cgctaaggtt aaaacaaacc
    18901 cacagtatct gtctaaacgg cagaagttgg atgaagcacg gcacatcgca gacatataca
    18961 aacatgctgt cgccgccttt tcgcatcgcc gagacatgat cgttcaggcg tcaaagatgg
    19021 ccatcgttga attagagcga ttaggctctg aacgctttat tactccccgt tgatttttga
    19081 tagataataa gtaagtactg atctatcatt taacagctcg aaagagccac gaatgaacga
    19141 aagcccaacg cgcatagcgc catcggccaa atcacaacaa ggagaaacac atgtctaaga
    19201 cattacttga tttgcttaac aaaactcgtg aagacattgc cgccaaacgt ggtaacaacg
    19261 ttgatctgac tcgcttaaaa gacggcgtca actatatccg catcttcccg aataaagacg
    19321 acccaaacgg taagttcttc cagactttcg gtatgcacta cgttaagtat cagaacgagg
    19381 aaggtaaaga agcaaccaac gcttatattt gtgagcaaca tactcacggt cgcgcttgtc
    19441 agctatgcga aatggtgatg gaaggtcgcg ctcgtcacaa gggtaacaaa gcaatggaag
    19501 aacgcatcgg tcaaatgcgt gccactcctc gctacctggt caacggcatt ctttctgctc
    19561 gtgaggattt cgcagatgct gagaaatgcc agttaatcga gctgccgtct actgtattcg
    19621 atgatatctg caaagcaatc accgaagaca tcgctgatga tatcggcaat ccactgagca
    19681 aagaggaagg ctacgcattc ctgattaaac gtactggctc tggtcgcgat accaaatatg
    19741 acgtctcgcc taagcgtaaa gtctacaaag gcgatatcga agataaattc tggaataccc
    19801 agcatgatct gatcgcatac gcaaatcagg ctgatgaaac tcgtcttctg tcgacagttc
    19861 gcactatggg tcgtctgatt ggcatcgctg caccaactgc cgcagcatct gcaccagcaa
    19921 tttcctcaac cgcgaaaaca tcggctgcgg cactacctgg atttggctct gtcactggtc
    19981 atacggaggg tgcgactgct gtagctaccg ctcatacccc agcttctgaa ccaaccagtc
    20041 tggttgatga agaaatcctc cgtgccgttg aaactgaatt taaaccagaa gcaagtcccg
    20101 ctgccgttgc agtatcagtc aaagagtctg aagcagtcgc agcgacatct gtagcagccg
    20161 catctgcgac ggaagatgaa ggtctggatg acctactgag agagctggac tctctgtaat
    20221 cccattacgt gaccagtaag gcgtctacgg acgccttact ttttggaagg aatgtaccgg
    20281 tgaattatct cttcgtagat ggcaatagcc tgggttatta ccaccaacaa tctgacaaat
    20341 tgcacaacgg cgaaatggaa gtacaggctg ctttcggctt tgttaagaac gtccgtcgtt
    20401 atgcctccat cctccatgcc cgacctatga ttctttggga tggatttagt gacaagcgtc
    20461 gcgactttta cccggactac aaagcaaatc gcgacgacga tcctgatatg aaaaagatga
    20521 aggaaggctt tgatatccag aagccataca tcctcaaaat gatgaccgcg cttggagtta
    20581 cccaactcat tgcaaaagat gcagaagcgg atgatctggc cgggctgctg gtatcccgca
    20641 tggcaccgca gccaaccgtt gaacacatct atctgttaac aggcgatagc gactggcttc
    20701 agttagttcg tgaaaacgta agctgggtaa gcctgcgtga agacgccaaa aacaagcagg
    20761 ttaattttga gcaatttgcg gagctgacag gattcgctac tcctcgcgca tttttggaag
    20821 caaaagcatt acaaggcgat aaatcggaca acattagcgg tgttggtggc attggtgctg
    20881 gcggtgcgaa agagctgctg catgaatggg gaagtgtcgc aacgatggta cgcggcatca
    20941 acgacggctc aatcgtggtt gacaaaggac gccataagac cgccttcaac aaactagcga
    21001 agaatgcctt caacgagaaa acaggctgtc gaatgctcga agcgttcaag agaaacatca
    21061 cgctaatgaa cctgattgag acgaagtttc cgcctaccga aatcgaaaca atcaaaggca
    21121 atcgtgacgt gaaagcattc gagcaactgt gctacgagct gaatttccgt tcgttccttg
    21181 aagaccttga agtgtttgtt cttccattcg aaaggtattg ctaatgctta aatcgattat
    21241 taatggcgct acaaccaccc ccacccaact ggcaaaagag attgtctttt atcacggtga
    21301 gtacgctgtc atcgcactgc cgtcaattct aggcgctgcc ggaatgaaag cgacagatcg
    21361 cgagtttgga ttagtcagcg agcaggtcgt aaaaatcctc gctcgtgtat ccagactcct
    21421 taaccacgat gcgattgtat tcgacgaatc cgccgcttta aaacgaatca acgaaacaaa
    21481 aggagcctga tcatggcaaa aggaaaatcc gcactggcac ttgctctgaa aaagaaaatc
    21541 ggtagcaacg acgaaattca gaaagtaact cattggattg atacaggctt tccaccgtta
    21601 aacaaagcta tttctggtcg ttacgatggc ggcttcccat gtggtcgtat cgtcgaagta
    21661 ttcggtcctc caagtgcagg gaaaacgttc cttgcaacag cagcgatgat ctccgctcaa
    21721 aaacaagatg gtctggcggt attcctcgat catgaaaaca gttttgacgt tggcctggct
    21781 gtagccaatg gcctgaacgc agatgaggat gacggtcagt gggtatacaa gcagcctgat
    21841 accttcgaag actctgtaga gttgatcggc acaattctta aattggtacg tgatgaagag
    21901 cttattcctg aatcagcacc tatctgtatc gtggctgact cacttgcgtc tatggttccg
    21961 aactccaaag ccgagaagtt cgaaaagatg gctgaaggca ctgccaaaga caaagatcag
    22021 ctaaacatga acgacaatac ggcactggct cgtgcgacaa gtgcgaactt ccctactctg
    22081 gctttgtggg cacgcaaata caacgcctgc atcatcttct tgaatcaggt tcgcacaaaa
    22141 atcggtgtaa tgtttggcga ccctactacg tcgccaggtg gagattcacc gaagttctac
    22201 gcttctgtac gtatccgtct tggtgcatcg gtgatgaagg atggtaaaga gaagatcggc
    22261 caggacgtag gcgcagaatg catcaaaaac aaagttgcac caccgtatgg caaatgcacc
    22321 tggaaattct acttcgatcc tactcgtggc ctcgacgtta tcgaatcgct cgtcgagtac
    22381 atgctggaag aaggatacct gccaaagaac gccagcgggc gagttgaaat tggtgacaag
    22441 aaatacacca aatcgcagat cgtcgagatg tatcgggaga agccactggc tgaaatcatt
    22501 gcggctttgc aggcaatcga cgaccgaaga gcaaaagaca accccaccga gtcagtagaa
    22561 gagtaaacac aaggcgtcca caggacgcct ttttttatct cttgaaaata tataagtact
    22621 tacttattat tttcgcataa caaccacata ggaaaacaca tgatcaaaat ctatctattg
    22681 gcagtagcca caggcgtttc agtggctctc atctacggtt tactggttcc gtcgctgatt
    22741 tctaccaaga gtgatttagc cgtcatgttt ggagttatcg ttggttttgg tgctcctgta
    22801 atcggtctta ttgctggtcg taagtttatc aactcattaa tcaaagcaaa ggggaaataa
    22861 gtaatgaaga aaggtttact tgcagttgct ctggcggcta tttgcacaat gggtcttact
    22921 ggctgtgatc gcgtggagcc tggatacgtt ggcatcaaag taaacaaatt aggtgaagac
    22981 aaagggatcg gtgaagtggt tggcgttggt cgccaatgga caggtcttaa caccgaactt
    23041 tacgtattcc cgaccttcaa acaaatgaag acctacgacg agccgttcac attccagatg
    23101 agtgacggta ctgctattgg tcacaaaatt ggcgttgcgt atctggttaa tcgtgacaag
    23161 gtaacgacgg tgttccagac ctatcgcaaa ggcgtagacg atatcaccga atcagatctg
    23221 cgtcagaaaa ttgccgactc tctaaaccgt ttggccagcc gtatgaccac tgactcattt
    23281 atcgacggtg gtaaagcgca attgctggac aacgcactga aagatattca gaaagagatg
    23341 tctccggttg gtattgaggt actgagcctg tcatgggttg gaaagcctga ttacccaaaa
    23401 accgtcattg aatctatcaa cgccaaagta acggctaacc agcgtactct gcaacgtcag
    23461 caggaagttg aacaacgtaa agctgaggcg aatatgctac gtgaacaggc taatggtgaa
    23521 gctgatgcta tccgtgctcg tgcgcaagca gaagcagacg ccattcgtct gcgcggtgaa
    23581 gctctgcgtc aaaacccgaa cgttatggaa ctggaagcca tcaataaatg gaatggccag
    23641 ttaccgcagt acatgactca aggggctaac actcctttca ttacagtgaa ataactcccc
    23701 taaaaagttc aggcgtccag ttggacgcct tttttatcgc aattatctta ttaagaaaat
    23761 aatttgttta aaaggataag aaaacatgac agctattaag aaactctacg atgccgcaaa
    23821 cgtggctctg gatgttattg atgatgaagc agcaaaaggc tttcctgaac ctgattgggc
    23881 gcatcagcta cgaaacgcta tcgcagaaat gaccccacca gatccaaccc ccgaggagac
    23941 agactggcag cgattcatcc gtatgtacgc tcaggaaata ggtccaacgc caacggcaga
    24001 gcaggcaatg ctgctgaaat acttcaaaga ggcgggagag gatttaccaa ttgatgactc
    24061 agcatattgg ttccactgcg catggcgtaa gtatgacgtg atattcacac aaggcatggg
    24121 aagcaaagat atggttgtgt ggcatctact ccatatagac acagccgttg acagagttat
    24181 tgaacagttt ttccctaaac aagaagattg atcgcctatt cataactaac aaaataagta
    24241 aacactaacc acaaaaggaa aaacacatga gagttttagt tcgaatcgtt accagcactg
    24301 tctatgacgt gtttccgctt tttatggtca aagtcgatgg ccttaacgat gaagaaactg
    24361 acgcgctgat ccagcgtact cttgttgaat atacaggtca tgacgctgat tcagtgatgg
    24421 ttgatgacga tggtgtttgt tggcataacg gcaactgttg gtacgtagaa gagactcaac
    24481 aaatcagtaa tgaagatgct gaacatcttg agcgtatttt aagcatcagc acttttgagt
    24541 gagtttacag taaagtttat ataagttagt atctacctat catgaagatt tttattgaat
    24601 acttgttact catcgtttca atagcttttg tcatcgactg cattttcacc ggtgtcattc
    24661 gtaaagtctt ttccccggtg cacgacgtag tcataaacgc tttggctatc gtgctcgtat
    24721 ttaattcagc atttgatgta atcaaagagg tggcagcatg aaggccatcc cattcgctct
    24781 gttgttcctt tcctcgatcg ttgtggccga caccactgtt tatcagtgtg aaatgtctgt
    24841 agccgacgtt aagaatggcg ctcttaccga cgtcataaaa gcaccatatg gagcgatggt
    24901 cgtagacagc ggcgaccagt tctatgttgt gcgtgacgat cgagtgttgt catccccata
    24961 tctcacaaat cgtaatggca aattaaccgg cgtcggagaa gaccacttcg tatacaacaa
    25021 atacaagggc ttctatggcg ttcacgcttc tcagcaaagc taccttttcg atgactgcaa
    25081 ggaggttgga taatggcatt aacactggca ggtctggaaa tcgagaaaac aagcggctac
    25141 tggcgtgcta agggtttcaa gcagcctggc attcttgagc gtctggaacg tgaagatggg
    25201 tatatcgtcc accagcggcg tgaatggcgt atgtacgatc cagaaacagg aaaactgact
    25261 acaaaagccg gaacactttg gggcctgttg aagaaaatac actaatagca ccaaccactg
    25321 cggtgagtag ccagctcacc gcattcgtat cagctcatta tccactgtgg tctgcagtcc
    25381 tctcttccta catatctgct aaccactgta gcgagtagac agcctttcat tcgcatcaaa
    25441 caaccactat ggtgagtatt ggagtgagat gtccagcggg tatccactat ggtgagtaag
    25501 cctttgctgt tttcagtgga tatccactcg ccacagtgga tacgctcaaa gatgagggcg
    25561 acaacaacca ctatagacag taaattttcc aagataccca gtgaccacta acctcgcagc
    25621 ccttgtttca tcagtgttag tgatcactaa catttgattc agtatttgca ttctactgcc
    25681 tacagtggtt atcaacagat aacgatactc actatagtgg atagcgaatt acagatataa
    25741 ataaggctca ctacagtgga tagtgagcct tactactaac tacagtggtt gggctatttg
    25801 cgagtctttg ccttgcgcaa ctcttcgaga agcgccagtt cttctttact caacatgacc
    25861 attttgcctt ctttttcgtc aggcacaacg tcgataatgt catcttgatt ctcatctggg
    25921 aggctatatt ccgtctcatc attatcgatt tttgttgata tttgtgcctg gcgtaacttt
    25981 ggccgcctat agtggatgac gaagtagacc gagtttccgc gcttaacttc cgtgtaatcg
    26041 agatatccta tctctcgcag ctgttccata gcctttctta ccgtcgcatt ctgagtaata
    26101 gtgcgacttg tcagattaag tctggcgcgt aaacgggcta gtgatattgg tgccggatca
    26161 ggcggcaaac tttcaataaa agtataaagc gcctgagcgg attcttttct tgagagttcg
    26221 tttatagccc ggagttgcag aagaaccttt ttgtcgaact ggtagagttc gaaaatcttg
    26281 ggatctgctt tcagttcgac cgtatcgtgc ttgacactat attttgccgt ttgcacaaga
    26341 tgcgttacgt aaaactcatc agagcctttg ctacggaacg agatggtatt tgtggcaata
    26401 cggcttagcg aactgtccag gcgtttgcgt aacttcgctg atgaccttgc ggtcggaatg
    26461 ccacacagcc ttacaaactc aacgaacggc aacgtgacag tatcgccaac gaccttgtgt
    26521 ttggcaaacg cgtggattat tcccacccac gttttaaagt cgttatccat atcaagccga
    26581 aggccagaga tccttatgtc ttcgtaccct tcggcttttg ccagagacag ctgtttgagt
    26641 tcagcagtgg catccataga gaccatttgc ccctttcttc ctctggatgt cgatttcagc
    26701 gtcggaacga agaggcccag acgcatcaaa gcaacaggct gaacagtgtt attagtgtta
    26761 ggaaccaacg taacaacttc gcctgtcttt ttatctgttt ctgaaaatgc ttcaacgatc
    26821 gctatgtttt tattgccgtt ttcgctcatt ccaaatgtct cttgttattc gacggctttg
    26881 gtggcctttg ctgattacag tggatagtag cactcatcac agcggttatc ctaccgtcta
    26941 tagtggtttt tctcctctct atagtggttg ctttgctccc tatagtggat tattcgctct
    27001 ctatagtgga tatcgatcat gtctgaaggc agatggcgca acggtttgag gtgggcgggg
    27061 atcttattgg gtctttatgg gtctctttgg ttctctttgt gatctgaatt actggatcgg
    27121 gcctgtggat aaaaatcaga ggatttcaag aacaggtaac tctactcttc tgtggatatt
    27181 taaggacata agaatcatga ctaagagtag agtttaaaat atatctatgt atatcagtat
    27241 gttaatgaag aattaagtca ttaagttatc cataaaagac aatgtgctct actctctaca
    27301 gtggatatat acagctctcc agagtggttg ttttactctt catagcggtt attcagctct
    27361 ctacagaggt ttctttgctc tccatagtgg atggcaacac cctttcagac cagtaaccgc
    27421 aacggctaga ggcgatcggg gatctctttg gatcttctat ttgatcttac ttgggatctc
    27481 ttatttggat ctatccagtg gataactggg ataagtaaaa caggcaaatg cagattaagg
    27541 tatgcctaat gagttatcgt agcatcggcc aataactact gaaaaaacga ttatggatct
    27601 aaaacgcacg cgctgggttc gtcgtcttga ggacggctcc tacactatcg aatcaaattc
    27661 caacctgaat aagcagaagt tactttgtga tatctgcggt atagcggcga agtgcccgat
    27721 ctacgaaacc agaattaaac ttgataaggc tggtgtgaac tttcatttga atagttgtat
    27781 caggtacgtt ccattgctcg catttcgtaa accgatcatc ggattggatg cgccctactt
    27841 caacacactc cgttcaggag taacgtggcg agatcgttta tcaccagaca aactgatttg
    27901 ccttgtatcc gcagatacag gaaaaatcat tcgttttggg aaagtagaca aggtttactc
    27961 aggcccagtt gacgagatgt tgcggaaaca cagccggttt aatcacctct gcatgggtgg
    28021 tgagaaaatc gagaaggttg aagaagtgat ccgcaaatcc tacggacact tcctgaccaa
    28081 agatagcctg ctcactgcaa tctacatcag acatgtaaaa cgtgagttcg accttgaata
    28141 ccatagcgaa gaagaactga accttgtcga tccacgtcct aaagctggcg tcataagcat
    28201 aaatgcagcg cgtaaaaagc ccactgacgc gctgtaaccc tccagatcgt atattggcgt
    28261 agatagaatc tacgccccct caaaatagct ctcatagcgt tctacagtga tccagtctta
    28321 tttttagtca tacagacaag caaagttgcg ccacgataaa taggtatata cttacttata
    28381 aattttgtat attaaggcgc tcgtttcatt cctaacatac cgttatgcat agttgtttac
    28441 ctcctcattg ctcttagaat ttgtatcaaa ataaccacaa aggaaaaaca catgactttg
    28501 ccatacggcg tcatttctga ctgccactac cacaaatggg atgcgttctc cacgacgaac
    28561 gctgaggggc ttaactccag acttgaaata cagttggaag caacgaaaga agcagccatc
    28621 gccatgaaga aggccggttg taagtacatg ttggttgccg gtgatacatt tcacgtccga
    28681 ggaactgtgt ccccttctgt tttgcattac gtaactgaaa cgtacaagtg gattatcaac
    28741 gagcttgatc tgacagtagt aatgctggcc ggtaatcacg atcttgaaac caacgattca
    28801 gtatatagcg ccaacgcagc agcatcgctg agttctatcg gcgtggtaat cgtatgtggc
    28861 aagcgcccac actcaataaa aattggtgat gtgactgtcc acctgattag ctggcgtaac
    28921 aatcatgcgg agcttatcag cgatctgaaa gcattacgta agagcgtaga aggtgataat
    28981 catgacgttg ttatccatac atccattaac aaagccattc caacaatgcc tgacgtcggt
    29041 atcgatgcgc aggagttaaa ggatatcggc tttcgtctcg tgcttagtgg gcattaccac
    29101 aaccacaaag aggtcattcc tggagttatc agtgtcggtg cactgactca tcaaaattgg
    29161 ggagatgttg gatctctggc tggctacatg atcgtaaacc cggacggcag tttcagtcac
    29221 tacgaaacca gtgcgcctaa attcattaac ctggaagatg atgttgctga tgaccaaatt
    29281 cgcggcaact acgtgcgttt ccgcgccgta attgagaacg atgaagaagg cattaagtac
    29341 cagaacatcc tcaaaacaat gggtgcaaaa ggtgtcgtgt gcaacttcat ccgtaagtca
    29401 tcaatgatgg aagggacagc cagcacaact gaaaccagca aaatcgatag cctgggagag
    29461 tcggtatctg cttattgcaa gattgtccac gatactgacg gcggatttga tctgagcaaa
    29521 ttggatattt tgtgtcagga aatcctcacc gaagcggaga gttcggaggc tgtgtgaagc
    29581 aaagtcgtta tgggagcttt cgagactttg ccatcacgat gaaaagactt gaacgaggcc
    29641 agacggtgat gtttcacaag ccctacccgc cacaaggaaa tcccgtagcg ttttatcttg
    29701 gaaggttaac aagaaaaggc gtattgaggc gcagatcctt cccggcgcat acggagttca
    29761 gattgaaaga aggccaaaag ctaacacacg gtatcagagg tgttatatga agtttttaaa
    29821 gctccaggtt gagaatttta tggctatcgc cagcgcggag gtcgagttag atcagcgtgg
    29881 tttagtgctc attcagggtg ttaatagtga tgatagttcc gcatcaagta atggctctgg
    29941 aaagtcaact ctaatgaata gcctgatgtg gtgtctttat ggcgaaacag ctcatggtgt
    30001 gaagggtgac gatgtgttgt ctaccgacca tgaaaagaac tgtcgtgttg cagtaaccat
    30061 cgaggatgaa ggcaagagat acgcaatcat tcgtcaccgt aaacacaaag agttcaaaaa
    30121 tcgtcttatc gttcgtggtg aagatggcga tatgacgaaa ggcaaagatg cgctgacgca
    30181 ggagttcgtc gagcgtctga tcggtgcatc taaagaggtt ttcatggctt ccatctatgc
    30241 gagccaagaa gctatgccag atttacctgg aatgtccgac aaaaatctca aaaccatcgt
    30301 agaagaagcc gctggcgttg acagactgac acgcgcctac gctattgctc gtgagcgagc
    30361 taatgcagct gccgcacgta tggatgtggt taaaaccaaa ttggagtcga caatctcgac
    30421 cattgaggca acacagtcag aaattgagtc agcgaaagcc tcctctgaat catgggagca
    30481 agagcgttct aaacgttatg acgatgccct ggctgggctg gccagtgccg aagttgagtt
    30541 aacggaagtt gaacttgaga tccgcactct tcccgaacag atacgtgata ccgagaaggc
    30601 aatcgaaaat gagcgcaaaa agttagcctc aaaagaagaa catgacgcca agttgctcaa
    30661 agttcgtggt gcgataactg atattcgggc aagcatcaaa gctacagaaa atagtcaggc
    30721 tgatgcaatg aaccgcgcgc gtaattttaa gaccaaagca gaagaggttg gtactaaagt
    30781 gggatcacca tgccctactt gtggcaaagc ctactgcgaa gaagatctat caacggtgaa
    30841 ggagagtttc attgaacaag cacgtcagga aattggtcag gcgaagacac ttgcagaggc
    30901 aatggctaaa cacaaaacga atcttgagaa agcgttaagc attgagtctg cccttgttaa
    30961 aacgacacct gatgtaacgg ctatcattgc ccggattgaa gagcttacga aacaactctc
    31021 atctttgcgt catcgtgaga aggaggttgt tgctattgag tctcttgtga ctcgtgctcg
    31081 tactgaggtc gatcgtatat caaaagagat taatccgttt attgctctta tcgccagaca
    31141 cgaagataac ctggtatcca gtaagtctac cttcaagtcc ttaaaagatg agttgaaggc
    31201 tattcaggaa caaacgttgc tattggaaaa agctcgtcag gtctactctc ctgccggggt
    31261 gcgttctcat attttgacgt ctgttacgcc tttcctgaat acacgcactg ccgagtatct
    31321 caatacgtta tctgacggga atattactgc tgagtggtcg acgatggatg tcactaaaaa
    31381 aggtgagtat cgcgacaaat tcaacattag tgtgcagaag aaaggttcaa gtaagtcgtt
    31441 ccagaccctc tctggtggtg agaagcggaa ggttcgcatt gcgtgttctt tggcattgca
    31501 ggatctggtt agtaaccggg cgagtaaaaa catcgatttg tttatcggcg acgaaattga
    31561 cgatgcactc gatacagccg gtcttgaacg cctcatgggt attctggagt ccaaagctcg
    31621 cgagcgaggt actgtgctga ttatctccca taaagagatg aagtcatggt tccgggaaac
    31681 tattacgctg gaagttaaag aggggcgcag ctatgtcgtt taaattaagc cgctcgcagt
    31741 ttttgcaggt atttgcagtg atgcagtcga taaaactgat caatgggcat acttccaatg
    31801 gtgcggctcc acgtattctg tggggaagca acaatattga cggagtacaa ttcgccgcgt
    31861 tgcttggtct aatatccgag acgccattga tgcaaagttt gaaatcacta ccacctggat
    31921 gtattgcgcc gatcctgatt aatccttttg ttgagggggg atatcttccc aacgtcgggc
    31981 ctgggtttat tgcatcccat gaaactgaag atcttaacat taatagcgaa gggttctttg
    32041 ggggaatggg tgcgcatcac tgtatggctt tcacgaacct tattcgactt gccaataagc
    32101 gggtggatag tttggcatcg ccaggtgatg cttttactgg tttccttatc caaaggaggg
    32161 ataaaaagta cagtgcggac aaactacagt ttgttggtaa gtatggagaa atggtagaaa
    32221 tcgaacttca gctccctcat gttttagcaa acgatagtgc agacagtcgg aggctgttgg
    32281 gcatcatgcg tcatttcata gcaagtggcg ttaaacatgc cgtagataaa cgtgtcacgc
    32341 aggaaaatga gtattcagac tttgcaaact atccccaacc aacgttgcaa acggcaatag
    32401 tagccaattc gttggaggcg agattattgg aaaaccctat atggggaaca tggtaaggag
    32461 actatatgag taaaaaaatc agcgtagttg gtgttgatcc ctcaatgagc aactttgggc
    32521 ttgctgtggg cactttagac cttgagacgg acgaacttga gattcacggc cttactcttg
    32581 ttgagactaa agcggggagt aacaaaaaga ccgttcgtgt gaacagtgac gatctgcgcc
    32641 gagccagtga aatatggcgt gttgcgaagc caatcattga taaggcaaat atggtttttt
    32701 gtgagctacc ggttgggagc caaaactctc gttcgcagac gtcttacggt atttgtatcg
    32761 gtgtacttgc gtgtgtggat aagccattga tccaggttac tccaaacgaa atcaagcatt
    32821 ttgtcggcaa taaacttact acatcgaaag aagagattat ccagtgggct acgaaaaaac
    32881 accctaaagc accgtggctg cgtcgtaagc aatctggaca ggatgttctc gtgaacaaaa
    32941 acgaacattt ggctgatgcg gtggctgcca tccataccgg tatgcaaaca gatcagttcc
    33001 gccaggtgcg cgatgttctt aagtctctca tttgatttca tagataggta agtgcttatc
    33061 tattaacatg ggccactata tttagtggcc ctctttattt ggtgatacat gataagcatc
    33121 gtaaaacgta acggccaaac agagccgtta tccgaagaaa aatacaaccg cgtcgtaatg
    33181 tatggcgtag aaggcattcg tggtgtaagc gcatccgctg tagcaatggg agctgcggcc
    33241 agcatttttg atgggattac caccagccag ttgcatgagg ctttggttaa atctgccgct
    33301 gatttgatct caccagaagc accaaattac tcacaggtgg ctgcccgcct gaacattttt
    33361 aaaatccgca aagatgcctt cggtcgttac gactatccga acttctacca acacattgtc
    33421 aagaacgtta acaagggcgt ttatgacaag gatttgctga cacattattc atttgaagag
    33481 atcgaagaac tcggcaatta cattaagcct aaacgtgacg atctttttgg ctatgcagct
    33541 acggtgcagt tgcaaagcaa atacctcgtt caaaaccgtg ttactggtga gattcacgaa
    33601 ggcccgcaac atatctatat gctggtgggc atgtgtctgt tccagaattg ggaagacgac
    33661 tgcgcgggca aaacacgtat ggagatggtc aaaggtttct atgacgttac aagtacgttc
    33721 aaactgtctc tgcccacacc aatcatggct ggcgtccgta ctccaacccg tcagttctcc
    33781 agttgtgtgc tgattgagtc tggcgatagt ctgaaaggta ttagtgcagc ttcagccgca
    33841 attatcgact acgtttcacg tcgagctgga attggtattg gttttggccg tatccgtgcg
    33901 ctgggcagtg agatccgcaa tggtgaagcc acccataccg gagttattcc attcctgaag
    33961 catttccaga ctgctgttaa atcttgctcg caaggtggtg tccgtggtgg cgcagcaaca
    34021 gcgttttacc cgatttggca tcttgaagtt gaaagtctgc tggtggtgaa aaataaccgt
    34081 ggtattgatg aaaaccgcgt tcgccatctt gattacggcg tcatgagtaa ccgtctaatg
    34141 taccgtcgac tcgtcagaag cgagaacatc actctgttca gcccgcatga tgtgcctgat
    34201 atgtacgaag ccttcttcac agaccaggat ctgtttgaaa agctgtacca taaatacgaa
    34261 gccgatgatt caattcgcaa gaagtcagta cctgccattg agctgttctc atctctgatg
    34321 caggaacgag cgtccacggg ccgaatttat attgcgaacg tcgatcatat taacgagcat
    34381 ggcgctttca ttcctgctct tgcacctgtt cgccagtcaa acctgtgcat ggagatcact
    34441 ctacccactc gtccactggc atttaccgac gacccgaacg gtgagatcgc gctatgcact
    34501 ttatccgctt ttaacctcgg agccatccgt tcactggagt ctcttaaaga ggtggcgttc
    34561 tatgccgttg ctgcactgga ttcgttactg gattaccaag actatccgat ggaggcagcc
    34621 gaagtgcctg ccaaagctcg tcgtagcttg ggaattggtg taaccaactt tgcttattac
    34681 ctggcaaaga atggcgttcg ttattctgat accgctggca ataaactggt gcatgaaacg
    34741 ttcgaagcta tccagtatta ccttcttgat gccagctgcc gacttgctga agcaaaaggt
    34801 gagtgtgact ggtttgagca gaccaagtac gcaattggtc agttgccgat cgaccattac
    34861 cgttcttcat tagacgaaag cggcgaaacc aactttgagt taaagatgcc gtgggaagaa
    34921 ctgcgtgaac gtattgcaaa atacggcctt cgcaactcca cactgacggc acaaatgcca
    34981 tgcgagactt ccagccagat cactaattcc accaacggca tcgaaccgcc tcgtggcccg
    35041 gtgtcggtga aatcttctaa ggacggcatc gttaagatgg tcgtgcctga gtttgaaaaa
    35101 ctgaaggaac agtatgaata cctgtgggat atgccggaca accgcggcta tctgacaaag
    35161 gtggcgatca tccagaagtt ctttgaccag gctatttcag ccaataccaa ctatgaccct
    35221 tctcgctttg aaggcgataa agtcccaatg atgacgctac tgtcagattt gcttctcgcc
    35281 tacaagatgg gagttaaaac gctttactac cacaacacca gagatggggc aggaaagcgt
    35341 gatgacgacg aaccgcagaa tccactgacg caagctgtag ccgtcgagcc agaagatgag
    35401 tgcgacggag cctgcaaaat ctgacatatg gtgggggata tccccacctt ctctttgatt
    35461 tgtaagcctt gtttaaacac ataagataac aacttgttta aacgcaccaa aaaagaaaaa
    35521 ggaaaaacac atgtcatatt caacgttccg tttgggtgct aatgatgcaa ccaaagagcc
    35581 tatgttcctc ggacaatctg tcaacgtggc acgttacgat cagcaaaaat accgtgattt
    35641 tgaaaagttg attgaacgtc aattgtcttt cttctggcgg ccggaagaag ttgatatttc
    35701 gagtgatcgt atcgacttca acacgaagct gcgggaccac gaacgtcaca tttttctgag
    35761 caatctccgt tatcaaacgt tactcgattc agttcaggga cgtagcccaa atgcaacgct
    35821 tctgccgctt atctctattc ctgaactgga aacgtgggtt gaaacatggt ctttctctga
    35881 gactatccat agccgcagct acacccacat tattcgtggc atggtggacg atccgagtat
    35941 tgtttttgac ggtattgtta cggatgaaga aatcatcaac cgagcgatca gtatctctgc
    36001 tgaatatgac aggctttatg ggatgacctg cgagcgccag tcgttaggtg agaaagagtt
    36061 tgaacgtctg tacgtaaatg aatatggctg ggagccatac cctttgcacc gtcagctttt
    36121 ccgcacgttg gtgtccatta atgcgcttga ggcgatccgt ttctatgtaa gttttgcatg
    36181 tacgtttgcc tttggcgaac ggaagttgct tgagggtaac accaaaatta tgcgctttat
    36241 tgcccgtgat gaagctctgc attgcgaagg aactgaacgc atgatccgct tcatgcgtac
    36301 cggtcgcgaa ggcttattgt ggaaagagat tgctgctgat gaagaaaacg tcatttacga
    36361 caccatgaaa tcagtcgccg aacaagaaat gaactgggca gactatctct tcaaagacgg
    36421 ttcgatgatt ggtttaaacg cggatattct gaagacctat gtaaaatacc gcaccaatct
    36481 ggctatgaat cgtcttggcc tgaaggcttt atttccagaa gttaccacag atccgctggt
    36541 ctggatgaac aagtggttgt taaccgacac actgcaaatt gcaccacaag aggcagagca
    36601 aagcacatat ctggtaggtc agatcgattc taccgtggat aaggcttctc taagccagtt
    36661 tgcagacctg taaaccgata caaagcatta tgtggcctgg caacgctggg ccacaatgga
    36721 tcacaagaat taagaaggaa caaaactagt atgaacttta ccaaactgac tgaccacctg
    36781 aaacttgcca ccgatcgact cattggattt aagccagaac catatgagtt gcatgaaggc
    36841 catggtgtag ccactgaaag tatttacaag atggtcgatc agtttcatga actcttccag
    36901 catccgagac gcgttatgcc gacaccagag ctgcttcgtc tccgtgcaag cctgattcat
    36961 gaagaagctg tagtggaagg tattccagcc gcaatgaatg gggatattga gcaactgctg
    37021 gatgcaatgg ccgacttttt atacgttggt gttggtacga tggtcgccat caaaggtggt
    37081 atttctaccg gcatgaccta ttacacgcag gaacagagca ttgatcgctt tatgcagaca
    37141 atttttgtgc ccggtaacac tgttttcgat gatatggcaa tgccatttca ggaagctcgt
    37201 gaggcgtcat gtatgctcga agagctggca gataaacttg agaacaagac tgttaaggat
    37261 tctgagctga ttcaggaact gcgccgtgtc atgaacaaaa tctatgtggc gtgcatgatg
    37321 acctatcgac tggctgattt cctcggtatc aatgtagtcg agctggttgg cgaaattcat
    37381 cggtctaaca tgacaaaatt atggcctgct gatgtagagg aacgtcgcca ggctgtggcc
    37441 aactgcaaat acgactcttc agacctggga tttcgccatg ctgatggtac cgataagatg
    37501 atcggttttc gaatttccga tggaaagatt ctgaagtctc caacctatag tgatgtcgat
    37561 ttatcctcct ttgttgagca agctaaagcc tcagcaatgt acggaatgat caaaaaataa
    37621 ttgtaggtag ttatctatct gtgtatattt aacatgcgta ataaaactct ggaatgacta
    37681 ttcgttttgg tggcctatgg ccaccatttt tttatctgtc tggtcttgtt ctctcaatga
    37741 atgtaaactc acacgatgaa taagtaatta cttatctttg tgaggttttt gtgtcactcc
    37801 ttttgaatcg agagcatacg aacggtcagg taacaaacgc atcgtatgca aaagttattg
    37861 agacggtgct taaaagcggc gtgcaggctg atgatcgcac aggcactggt actttaagca
    37921 cctgctacgt tccctcttac tacatgctta ctggtgggac tgtgccgctt atttctggaa
    37981 aggcggtaaa tcttaagcca ctacttgtcg aacttgagtg gtatctgaaa ggcacgggca
    38041 acatccaatt tctcaaggat aacggcgtta agatttggga tgcatgggcc gatgagaatg
    38101 gcgatttggg gccggtttac ggtaagcagt ggcgtcgatg ggaagatacc cgcatcgtga
    38161 gccatagtga atatctgagc aagatcgcta ctttccgtga acgcgggtac aaagtcgagg
    38221 gatacctggg tatcagtgaa gatcgcgtag tgctgtcccg tgaaatcgat cagctacagc
    38281 gtattgtcga tacactgcgc acgaacccta ccgatcgtcg catcatgctt aacgcatgga
    38341 acgtaggcga gcttgaggat atgaaactgc caccttgcca ctttgtcttc tctttgtgga
    38401 gtcgtgagct ggattttgaa acccgtttaa cgatggcaac tgacattggt cttcaacaca
    38461 gtcgcctcgg ttacgagtct atctacacca agatgctata cgatctggag atggacggca
    38521 gtgttactga agctgaactg gatgaacttg gaatccccaa acgcatcctc aactcctgcc
    38581 tcgtacagcg tagcgtagac acttttgttg gtatgccatt caatattgct ggctatggca
    38641 ttctcactca ttttctcgcg aagattacgg gtcacatggc cggtgcattt gtgcattttg
    38701 gctttgacgt gcatttgtac aacaaccaca tggaaggtgt gtgtgagcta atgaaacgac
    38761 aggctccaga gcattcagat ccggtcgtta ttttccctca tgaatggtca gagttggatg
    38821 atttcaaatg ggacgaggtt ttaattcttg gctatgaccc tctaccgtgg atcaaggttc
    38881 cagtggcggt gtgatatggc aagaggtatg tatgtcttat gcgaaattga aggtgtgctg
    38941 gcaaatgcca gccatcgtaa atcagtatct gacgcggatg caggccagct cattgccggt
    39001 gatgaactca ttttccccac cagccgtatg ttgcgtggtt ttgctcgctc aggggctgaa
    39061 gtggtgctta tcagtagccg ctctgaaact cttgaagcgc caactaaacg atggctgaaa
    39121 gattttggcg ttgattatga ctggcttcat cttgtaccga atggcaccag ttatgagaag
    39181 catattaagc gcacattagc ggagcataaa ggcgatctgc ttatcgctgc gctggtgcac
    39241 gatcctcgac tccgtgccgc tttagccgac tctcaccatc gaccggtcat ctatgaggtg
    39301 agcaaatgaa gatgatcgct gctgttggcc gtaactatga gatcggcata gcgaatgaac
    39361 tcccctggcg ttgttctacc gatctgaagc tatttaagag actcaccaaa aacgccactg
    39421 tcgttatggg gcgtaaaacg atggaaagtc tcaaacgccc tcttccagag cgtcataacc
    39481 tcgttttgac gcgctctcat ggctttgtac caaatggatt ctaccctgct ggtgtggatg
    39541 atgtgttgcg attaccagag cctgtgtggg tgattggcgg ggaacaaatt tactcgctat
    39601 tcatgccgca tgttgaagag atttggctct cccacatcgg cgttgatgta ccaaacgccg
    39661 atgcattctt cccggcaagc atgatgcgta acttaggctt tgtgcctgtt gaaacagctt
    39721 atacccaacg agccagcgag gaagagcctg gcttttcgca gatcgtatac agaaggtcgt
    39781 aatggattac cggattggga tcactggtgc tcagggcagt gggaaaacaa ccctggcaaa
    39841 atatatcgac aaacattacg gaatccctta cgtggatgct ggtgtcggaa gtttgatgag
    39901 ccgcctcggt gttcgagtag gtgagtctat gcctctatat gagcggcttc agattcaaat
    39961 ggaaatagca aagcatatag agctacttac gcgtggtgtt gaaggctttg ttatcgatcg
    40021 cacacctgct gatgttatgg cctacacgtt ggatttggtc ggccatacca atgaagatcg
    40081 atgtattgag ttagccctcg atatcgaaaa gttttgccac aaaactgcta tttcaaactt
    40141 taacgccatt gctggcttac gcccgggagt cgctctctca gagcgagatt acttgcgatc
    40201 acaacgagca tcattagacc gtctgtatgt cgctcgtatt gatgcgttga tgtgcgggga
    40261 actgacaaaa attcacctgc atccgcaaag gggggatctg caaaccttcg tcgtttccaa
    40321 ccgatatcgc acggttgaag caagagccag atcagtgatg agaatgctag ataacgctgt
    40381 agaaaagata gaaaaccggt tctgtggccg agtgaccgtt cattagaaat tgttcgcctc
    40441 ttcgacattg cgacaataaa actctcaaaa tgggttaagg ataaaaaatg tttagtgaaa
    40501 tgttgcttga agatgaactg gatcggaaaa caacagaggc tttgattcgt gtagcggacg
    40561 aacattcccg gtcgcttatg agcgatcgag aggctcgtct ggctattcgt gccatattcg
    40621 aaactgcgca ggggcttgtt ggcgcacaag taggtgaagc cattaacatc gccatgtctc
    40681 agttcagtga agacagtaaa aagcctctgt ttcctatgca tttgatgctg gctggtggca
    40741 cggtgcttta tatctctgtt tgtctggata gcaaccaaat caatattctc aacactgcgt
    40801 caggtaagtg gaaagatccg attgtctgtg aaaccagtga agaaactttg aaaaaagcgg
    40861 ctcaatttgt acgtagcgca ctacttaagg gcgctaagaa gttgtaagga gttctgatga
    40921 caacgattgt tgcaggcatc gatatcgagt ctacgggact ggatttcctt gctggtcata
    40981 aaattattga aatcgcaatt acccgctatg aactggagac acagagacat attgatagtc
    41041 tggagatgcg ttttaaccct cgcagaaaca tagatccgaa agctcaagcc gttcatggca
    41101 tttcattaga acagctcgca gctgaacctt tgttgtcaaa tcatgccagc gaaattggcg
    41161 cttatatggg ggcatgtagt gcgtggattg ctcataacgg cgaagcgttt gatataccat
    41221 ttattcgaca cgagttttca gggtatggag taagactgcc agacgttccc gttatagata
    41281 ctatgttatc gggattgtgg gccacagaag acggtaaacg tcctcgcctt gaagagttgg
    41341 ccttctctct tggctttata tacgatcatg ccaaagcaca tagtgcctta tatgacacaa
    41401 acttaatgat gcaatgcttc tttaaggcac gtaataagta cggatttttt aaattaccct
    41461 ctgaaattgt gtaaaacaaa agcctacttt aaagagagtt taaagtaggc tttcttttaa
    41521 agaacagccg cctttcaatc atttcctgcc tgtatttaat acttttccgc ctgataggtt
    41581 tagtcaaaat gtagctatcg aaacgcaaat gtaaccaagc agaaggagac ttacatgagt
    41641 tcggttgaaa atgtaatgac aaatgatgat ctggacgagc tgacagccat gttgcaatca
    41701 cttgatgaac cagtaaaaaa agccgcacag gttgaaaata ctgatgatat tgacgatctg
    41761 ctgctcggcc tcgatgctgg cgtagccatg agttctgatg atgttgccga agaactgttc
    41821 aatgaagaaa aagcaggtga tttcagctct gctttaaatg agttggagtt agcgcatgag
    41881 cctataaacg taattaacgc tgaaagtgtt gaatctgccg aaaacgagcc agaacaattg
    41941 ggattgattg aggttgaaga gtgtgttgat gttaatgatg aattaaaagt tcaacagtca
    42001 aatgatagca acacaaataa aaaagcgcgt actgcaagag gtcctcgttt tactttaagt
    42061 gataaagatg attcgttttt caataaagca ggcttagaaa cagatatttt cttagacgct
    42121 tacgaaaacg cgcctgtcaa agcaaaggat aagatattaa accttcttaa ttggtttagc
    42181 ggaggtccag atattagtgt ttacacggta atttccatga gacaccttct cacagaaaag
    42241 aaggctacaa gtaatagtat taagattgct ttaatgagca atccagaaaa accgtatccg
    42301 cttaacactg cgtcaactca ggctgggcaa atgatggctg tatttccagc gacaggaatt
    42361 gccgttagag acggtggaaa tctaacattg aacgaagaat caccgatcgt taagaagttt
    42421 gtagcggagt acactattgg atgacgtccc cctactgaaa ataaagccca tagagagctt
    42481 tatagtgctg ggtaagccaa tcacatatcc agcaccacaa aaacgcgcca gagagcttct
    42541 cgtttgcatt tctggcgcgt tttatttgat tgccagacat aaaatcaaat gcaaaaatag
    42601 gtaatcactt acctatcgag aaagacgatg attgcagccg aaaaaatcaa acagcgaaag
    42661 cgcgacaact ctcttcgtga cctctggaga acacctgact ggctgttttc tgccattcaa
    42721 cgttatcttg gagtgacatt tgatgttgac gttgcctgca acaaggacaa tgcgaagctg
    42781 cctaatttca taggcgttga gcgtgatgct ttgaaatctg aatggggaca gccaggtaca
    42841 attgccttcc tcaatccacc ctactccaaa atctccccct ggattgatgc ggctatacgt
    42901 gagcaggctc gcggagttac aacagtgatg ctaattcctc aatccctcga tacaaagtgg
    42961 tatgagcgtg caacagagtg tgcgaatgag acgattattc tgtctggtgg ccgcgtagcg
    43021 tttgtcgagc ctgacgtcaa tttgggtcag gtagaagtaa acatcaaccc cggtggcagt
    43081 atgctcgttg tttttcgagg attctgtcag gacgctgggc actctataag caagatccct
    43141 ttggacgtca tgaaaagtct gggagggtat gatcctgcga atgtgatcag gaaaaaaaga
    43201 caatcaaaga aggctgctta gtttgttctg gcgtctgtaa ttagcctgct tctgtatata
    43261 taaataacta catattaatt attaatatac ggaagcaggc tgttttatat cagagactcc
    43321 cagacctgaa caccactaca gaatccacta gaaccccttc ccagacgctt taaaatcgat
    43381 tttatgaacc actttaagga aaccaacatg tcatacccga ctaatgtcgt tgcgctcgta
    43441 gagagcgatt ttctggccca ggctcgtgaa atgatgaaag atcgtgagca ggctttcaac
    43501 ttgtacgaat gggcaattaa gtgcttgcat cttggggagc atcgcgaact tgttgaacag
    43561 cttttaggtg agttgatcaa cgaggtgttt gccttgaatg ttcaactaca tggtcgaaaa
    43621 aataatcaat cgaaatgata gataagtaca aactattcat aaagtgaatt gtaagtgcta
    43681 agatctgata gtttccagtc gtagactgga ggctcgacct gatgggtggg ggtaagcgtc
    43741 actggcgtca ggtttaaaaa agctcactac cagcgtagaa ccggcaccgt ttaggggttg
    43801 gggaaggggg aaccaaagtg agcagagaca agggtcactt tatgattgtc gagtctgggg
    43861 tgtttcgaga ggttgaatcc agtactcccc ttcataaagt gtgggaagat ctcggttctg
    43921 gggtgctgtc atccataact tcccaagcct aagctggcag tagacttagg tcataacttt
    43981 tcaggttatg aaacgaccag gttggtgagg aaattttgta ctcacctccc tgggagagta
    44041 ttacctgaaa agacaacctc tcacttcgtt cgaggtgaac ttcactcact tcgttcgttc
    44101 agttcaggtt tataaaaacc tgttctggga agtaatttgt ttattttaat aattattaac
    44161 acgcacgcgt gtgcgcacgc gcgaggaaaa aaaaatcggc gcggcgcttg attcaggagt
    44221 ttatatgacg acgaagacac cagcccgatc gcaagcaaaa actcgcaaaa atgacaaaaa
    44281 caaaaattct ccccgcacca attccacaac gcctgtcgta gagttcaatc cccagcttaa
    44341 aaccgtgaaa atcttcagtg atggctcttg ccttaaaaat ccgggtggcc cgggcggtta
    44401 cggtatagtt ctccagtatg gtggtgagga acgcgagttc tcagatggtt ttcatagcac
    44461 caccaataac cgcatggaga tgatgggggc acttatcggg ctggagcgtt tgaaatatcc
    44521 atgcaacgtt attttgtact ctgatagcca gtatctgaaa aacggcatga cacagtggat
    44581 gaaatggtgg aaacgcaatg gatggatgac ttctgacaaa aaaccggtaa agaatgttga
    44641 tctgtggaag cgtctggatg aggccgcaag tcgacataat gttcgctgga agtgggttaa
    44701 aggtcacgcc gggcatcgtg aaaatgaaat atgtgatcga ctcgcgaaga tcgcagcttt
    44761 ttcagcagca gatatgcctc acaagaaaga tattggtttt gtttataaca agtagtaagt
    44821 aagtgtttac ctatcatttt aaatcatgta tcttatcagc gtcaggatga caatgtgtcg
    44881 gtaagacaca gttccaggat ggaacg
//
LOCUS       DAZGVQ010000020        44780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000020 DAZGVQ010000000
VERSION     DAZGVQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.020"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(269..811)
                     /gene="ycfP"
                     /locus_tag="ACHLAG_001085"
     CDS             complement(269..811)
                     /gene="ycfP"
                     /locus_tag="ACHLAG_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFP0654356.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(834..1859)
                     /gene="nagZ"
                     /locus_tag="ACHLAG_001086"
     CDS             complement(834..1859)
                     /gene="nagZ"
                     /locus_tag="ACHLAG_001086"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFP0654357.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(1870..2694)
                     /gene="thiK"
                     /locus_tag="ACHLAG_001087"
     CDS             complement(1870..2694)
                     /gene="thiK"
                     /locus_tag="ACHLAG_001087"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFP0654358.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2675..3316)
                     /gene="lpoB"
                     /locus_tag="ACHLAG_001088"
     CDS             complement(2675..3316)
                     /gene="lpoB"
                     /locus_tag="ACHLAG_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFP0654359.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3330..3707)
                     /locus_tag="ACHLAG_001089"
     CDS             complement(3330..3707)
                     /locus_tag="ACHLAG_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFP0654360.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3710..4069)
                     /gene="hinT"
                     /locus_tag="ACHLAG_001090"
     CDS             complement(3710..4069)
                     /gene="hinT"
                     /locus_tag="ACHLAG_001090"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFP0654361.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4403..6592
                     /gene="fhuE"
                     /locus_tag="ACHLAG_001091"
                     /pseudo
     CDS             4403..6592
                     /gene="fhuE"
                     /locus_tag="ACHLAG_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(6652..8085)
                     /gene="ptsG"
                     /locus_tag="ACHLAG_001092"
     CDS             complement(6652..8085)
                     /gene="ptsG"
                     /locus_tag="ACHLAG_001092"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFP0654362.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8380..9177)
                     /locus_tag="ACHLAG_001093"
     CDS             complement(8380..9177)
                     /locus_tag="ACHLAG_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0654363.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDVSRLQSIR"
     gene            complement(9188..10192)
                     /gene="holB"
                     /locus_tag="ACHLAG_001094"
     CDS             complement(9188..10192)
                     /gene="holB"
                     /locus_tag="ACHLAG_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFP0654364.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNMLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10189..10830)
                     /gene="tmk"
                     /locus_tag="ACHLAG_001095"
     CDS             complement(10189..10830)
                     /gene="tmk"
                     /locus_tag="ACHLAG_001095"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP0654365.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10820..11842)
                     /gene="yceG"
                     /locus_tag="ACHLAG_001096"
     CDS             complement(10820..11842)
                     /gene="yceG"
                     /locus_tag="ACHLAG_001096"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFP0654366.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKQAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11845..12654)
                     /gene="pabC"
                     /locus_tag="ACHLAG_001097"
     CDS             complement(11845..12654)
                     /gene="pabC"
                     /locus_tag="ACHLAG_001097"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFP0654367.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12774..14015)
                     /gene="fabF"
                     /locus_tag="ACHLAG_001098"
     CDS             complement(12774..14015)
                     /gene="fabF"
                     /locus_tag="ACHLAG_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFP0654368.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14103..14339)
                     /gene="acpP"
                     /locus_tag="ACHLAG_001099"
     CDS             complement(14103..14339)
                     /gene="acpP"
                     /locus_tag="ACHLAG_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP0654369.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14550..15284)
                     /gene="fabG"
                     /locus_tag="ACHLAG_001100"
     CDS             complement(14550..15284)
                     /gene="fabG"
                     /locus_tag="ACHLAG_001100"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFP0654370.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15297..16226)
                     /gene="fabD"
                     /locus_tag="ACHLAG_001101"
     CDS             complement(15297..16226)
                     /gene="fabD"
                     /locus_tag="ACHLAG_001101"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP0654371.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16242..17195)
                     /gene="fabH"
                     /locus_tag="ACHLAG_001102"
     CDS             complement(16242..17195)
                     /gene="fabH"
                     /locus_tag="ACHLAG_001102"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFP0654372.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17263..18333)
                     /gene="plsX"
                     /locus_tag="ACHLAG_001103"
     CDS             complement(17263..18333)
                     /gene="plsX"
                     /locus_tag="ACHLAG_001103"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFP0654373.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18414..18587)
                     /gene="rpmF"
                     /locus_tag="ACHLAG_001104"
     CDS             complement(18414..18587)
                     /gene="rpmF"
                     /locus_tag="ACHLAG_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP0654374.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18639..19160)
                     /gene="yceD"
                     /locus_tag="ACHLAG_001105"
     CDS             complement(18639..19160)
                     /gene="yceD"
                     /locus_tag="ACHLAG_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFP0654375.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19359..19943
                     /gene="yceF"
                     /locus_tag="ACHLAG_001106"
     CDS             19359..19943
                     /gene="yceF"
                     /locus_tag="ACHLAG_001106"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFP0654376.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20055..21014)
                     /gene="rluC"
                     /locus_tag="ACHLAG_001107"
     CDS             complement(20055..21014)
                     /gene="rluC"
                     /locus_tag="ACHLAG_001107"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFP0654377.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21587..24772
                     /gene="rne"
                     /locus_tag="ACHLAG_001108"
     CDS             21587..24772
                     /gene="rne"
                     /locus_tag="ACHLAG_001108"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFP0654378.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(24969..25922)
                     /gene="flgL"
                     /locus_tag="ACHLAG_001109"
     CDS             complement(24969..25922)
                     /gene="flgL"
                     /locus_tag="ACHLAG_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFP0654379.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(25934..27577)
                     /gene="flgK"
                     /locus_tag="ACHLAG_001110"
     CDS             complement(25934..27577)
                     /gene="flgK"
                     /locus_tag="ACHLAG_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFP0654380.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTATPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27643..28584)
                     /gene="flgJ"
                     /locus_tag="ACHLAG_001111"
     CDS             complement(27643..28584)
                     /gene="flgJ"
                     /locus_tag="ACHLAG_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFP0654381.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAVPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28584..29681)
                     /gene="flgI"
                     /locus_tag="ACHLAG_001112"
     CDS             complement(28584..29681)
                     /gene="flgI"
                     /locus_tag="ACHLAG_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFP0654382.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29693..30391)
                     /gene="flgH"
                     /locus_tag="ACHLAG_001113"
     CDS             complement(29693..30391)
                     /gene="flgH"
                     /locus_tag="ACHLAG_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFP0654383.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30444..31226)
                     /gene="flgG"
                     /locus_tag="ACHLAG_001114"
     CDS             complement(30444..31226)
                     /gene="flgG"
                     /locus_tag="ACHLAG_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFP0654384.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31364..32119)
                     /gene="flgF"
                     /locus_tag="ACHLAG_001115"
     CDS             complement(31364..32119)
                     /gene="flgF"
                     /locus_tag="ACHLAG_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFP0654385.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32139..33344)
                     /gene="flgE"
                     /locus_tag="ACHLAG_001116"
     CDS             complement(32139..33344)
                     /gene="flgE"
                     /locus_tag="ACHLAG_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFP0654386.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(33369..34064)
                     /gene="flgD"
                     /locus_tag="ACHLAG_001117"
     CDS             complement(33369..34064)
                     /gene="flgD"
                     /locus_tag="ACHLAG_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFP0654387.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNRGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34076..34480)
                     /gene="flgC"
                     /locus_tag="ACHLAG_001118"
     CDS             complement(34076..34480)
                     /gene="flgC"
                     /locus_tag="ACHLAG_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFP0654388.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34484..34900)
                     /gene="flgB"
                     /locus_tag="ACHLAG_001119"
     CDS             complement(34484..34900)
                     /gene="flgB"
                     /locus_tag="ACHLAG_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFP0654389.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35055..35714
                     /gene="flgA"
                     /locus_tag="ACHLAG_001120"
     CDS             35055..35714
                     /gene="flgA"
                     /locus_tag="ACHLAG_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFP0654390.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            35790..36083
                     /gene="flgM"
                     /locus_tag="ACHLAG_001121"
     CDS             35790..36083
                     /gene="flgM"
                     /locus_tag="ACHLAG_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFP0654391.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36088..36504
                     /gene="flgN"
                     /locus_tag="ACHLAG_001122"
     CDS             36088..36504
                     /gene="flgN"
                     /locus_tag="ACHLAG_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFP0654392.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(36544..38079)
                     /gene="murJ"
                     /locus_tag="ACHLAG_001123"
     CDS             complement(36544..38079)
                     /gene="murJ"
                     /locus_tag="ACHLAG_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFP0654393.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38189..39112)
                     /locus_tag="ACHLAG_001124"
     CDS             complement(38189..39112)
                     /locus_tag="ACHLAG_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP0654394.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39114..39761)
                     /locus_tag="ACHLAG_001125"
     CDS             complement(39114..39761)
                     /locus_tag="ACHLAG_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFP0654395.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(39772..40356)
                     /gene="rimJ"
                     /locus_tag="ACHLAG_001126"
     CDS             complement(39772..40356)
                     /gene="rimJ"
                     /locus_tag="ACHLAG_001126"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFP0654396.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40592..41800
                     /gene="mdtH"
                     /locus_tag="ACHLAG_001127"
     CDS             40592..41800
                     /gene="mdtH"
                     /locus_tag="ACHLAG_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFP0654397.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVHTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41864..42511
                     /gene="grxB"
                     /locus_tag="ACHLAG_001128"
     CDS             41864..42511
                     /gene="grxB"
                     /locus_tag="ACHLAG_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFP0654398.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42645..43204
                     /locus_tag="ACHLAG_001129"
                     /pseudo
     CDS             42645..43204
                     /locus_tag="ACHLAG_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
     gene            43310..44356
                     /gene="pyrC"
                     /locus_tag="ACHLAG_001130"
     CDS             43310..44356
                     /gene="pyrC"
                     /locus_tag="ACHLAG_001130"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP0654399.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44430..44675
                     /gene="dinI"
                     /locus_tag="ACHLAG_001131"
     CDS             44430..44675
                     /gene="dinI"
                     /locus_tag="ACHLAG_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFP0654400.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT        10812 a        12106 c        11155 g        10707 t
ORIGIN      
        1 atgttaatca agttttgttg ctgaaaagat aggcgacttt accgggtgag atcaaaaaat
       61 agagtttatt gtttattatt tgagcaaatt cggaagagta atatagggtg aagcgtatat
      121 cgagggcgtt tggtcgtcct gataagatgc gcagcatcgc atcaggcatt gatgctcgac
      181 tgtcggatgc ggcgtggacg ccttatccga cttacagaat tcggagcgca tcaggtaccg
      241 gtaaggttac ctgatgcgac gagggcattt acccgagggt tttgaacgct ttaatgcgct
      301 gtaaatgcgg ggagatattc ttgaatttgt gcgtctgctc ttcgtcccag acaatctcgt
      361 aataatgatg caactcttca gatgtccgct ggctgttaag tgcttcatca ttacgcgaca
      421 aaataaccag acaacgatcg cgattcttct cgcggaagtt ggtcacacac ttagtggcaa
      481 tatcggcata ctcttccggg cgatcaatct tgccttccat gttctcataa gggaacaaat
      541 tagggttgaa gatcacctgg cggatatcgc aaagaaaacc aatccgttcc gcccagtatc
      601 cgcccaggcc aacgccgcaa attagcggac gctcgtcaac gttcagttgc aacattttgt
      661 ccacttcttt aagcagatgc tgcatatcat gtttcggatg ccgcgtactg tagcttatca
      721 agcgtacatc cgggtcaata aactgcaatt gtaagacttt ctcgtggtta cccggactgt
      781 tagagtcaaa accgtgtaaa tagataatca tcgcttcctc acataagcca gggttagtga
      841 cctgctttct cttcctgcca gcgttcgtgt aactgattca gacgggtgct gatcgctttc
      901 cagcgagccg agtccatcag ttcctgtcgc gaaaatgaac ctttatgata caaacgtgta
      961 acacgttctg ccttgatcgg tgacagatta tctaacacgc tgacggcccc tttacgatta
     1021 ttgcagacca ggatcatatc gcaacccgca tccagtgatg cctgcccgcg ttcggcataa
     1081 ctgcccataa tcgcggcacc ttccatcgat aaatcgtcag agaaaatcac gccgtcaaaa
     1141 cccagttcct gacgcaaaac ggttttcagc cagtagggag aaccgctcgc cggacgcgga
     1201 tcaacatcac tgtagatcac atgcgcaggc ataatggcgt cgagtttatt ttcgcggatt
     1261 aaggaactga agaccgacat atctttagcg cgaatctccg cttgtggacg tggatcgcac
     1321 ggtgtttctt tgtgtgagtc tgccgttact gcaccgtgtc ctgggaagtg tttcccggtc
     1381 gttttcattc cggcttcatg cataccatca ataaaccggc tggcaattgc cagggctttt
     1441 tgtggatcgg catgataaga acgctcgcca atcgccgcgc tgatatgccc gacatccagc
     1501 actggcgcaa agctgatatc aatatccata gcgatcattt cgctggccat cagccaaccc
     1561 gcctcttgcg ccagtttgcc gccctcttcc attcctgaca gcgcagcgaa tgattgcgcc
     1621 gctggcaagc gggtaaaacc ttcacgaaaa cgctgcacgc gtccaccttc ctgatctacc
     1681 gccaccacca gatgattgcg cgaagctgcg cggatctggc gcaccagttc acgtaactgg
     1741 gcaggatcat gatagttacg cgtaaagaga atcagccctc ccaccagcgg atgcgcaagt
     1801 atttcacgct cttccgcgtc cagttcgtaa cctttgacat ccaacattac tggacccaca
     1861 ctgctctcct tattgtttta ttaatagctg ccgccaggtg tcatcggcca gcctgataaa
     1921 ttgttgatcg ccggtttgtc gccagcggta ctcaaaccac cctgctttga gcatcagcag
     1981 ccagggaaac catcgcctga cctgacgcca taattgcgcc ggataaatct tcgcgcgagt
     2041 ggcatagtca ttgaccaatt gccggtgctg ttcagtattt tccacccaca ccgccgccag
     2101 ttccagcgcg atatcaccat ctccggcata ctcccagtcg atgagtttta accctgacgc
     2161 gctatgcact aaatttccgg cgtggacatc catatgcaat ggacttaagc gtaaaggccg
     2221 tggttcccgc gctttgcgca gacgttttaa cattcgcagc caacccactg tccgccgcgc
     2281 cggatcgctt tgctgccagt acagttccag taacggcaac agcgttattc gccagccaaa
     2341 acgtggttgt tgatgtagat aatacagcaa gcctgccagt tcgttggtat ccggcaaata
     2401 cgtttttacc gcgccgggca gatagtcgac taccatccag tcacggagat ataaatgcgg
     2461 cttcggtgca atgcttgcgg gtagttgtga taaagcccga tactggcgca agaacgcgga
     2521 ctgcggcgca tcaggatcgt gcggctgacg cacaacaaaa cgctggccct gatgttcaat
     2581 gagaaaactc ccgccactaa gcccactatt aaacgtcgtg acgggatgaa actgcgggaa
     2641 aaagcgcgac agcaattcgt cgcgcatgat gggattattg ctgcgaaacg gcacctttac
     2701 ctgaccagat aatttcgccc gtctgcacca gcatcagctg catttgtagg gtcggagcgt
     2761 taacgttgcc agaggcgctg gagtacagca cgtaatgagc gccgacattg cgggcaatgc
     2821 ctatggcttt actacgggta cctaaactgt cctgcggcga caaacctaac tgttgcttag
     2881 ccatcgacag ctgctgggcg gaaaccaggg taaatttccc gttattagcc agcgcatttc
     2941 gcagcgtttc ggtcgcttct gcggcattca gcgaaccgtt agtacggttg ttaacgctat
     3001 caaccagcag gacgctaccc gcagtcaccc cgtcagcccc aagcatctta ctgaccatcg
     3061 gctgcattgc gccattccag tcataatggc gaatatgcgg cgcaggcggt gccgtttgat
     3121 cttcgtgctc aattgggcct ggctgctgcg ggatcgtcgg caccgagggc actgtgggga
     3181 caggctgttg tggctcggct ggttgttccg gcgctggttt cacttcttct accggtgcag
     3241 gttcacgttg ccccacacac ccggcgagaa acatcgccag cgcggtaatc aaggcgtagc
     3301 gactcatttt tgtcatcaag attcacccct tacaaatata gataaagtct gactttgtgc
     3361 gcccccagaa aattggcgct gccgtacagc gttaccgccg aatgtgcggg aatgctaacg
     3421 ctgcgtggcc tttccagagg atgcatctcc agccctctgg cgtcatacca gtaaaaacga
     3481 taatgaacgg taacgggttc ttgcctttcg ttataaagcg ttgaggatgc tgaaggttga
     3541 atatcagacg tcgaaaggac gggcttttct gcactgatgc ccgcagccaa taaagatgac
     3601 tccatcacca gcgattgctc atcattcacc ggaatttccg gatgtgaacg acagcccacc
     3661 agcagtaaca acgccagaga caccagccca aagcatcctt ttctcatcgt tacagacctt
     3721 tatgcgccag cattggtccc agcggacggc cacccaacaa gtgcatatgg atgtggtaaa
     3781 cctcttgtcc gccatggcgg ttggtattca tgatcagacg atagccatct tcggcaatac
     3841 cttcttgctc agcaattttt gccgctacgg tgatcatgcg tcccagcgcc tgctcatgct
     3901 cagctgacac gtcgttcaca gtcggaatga ggatattcgg aatgatcaga atatgcgttg
     3961 gcgcttgcgg cgaaatatcg cgaaacgccg ttaccagatc atcctggtag acgatatcgg
     4021 aggggatctc acgacgaata attttgctga atatagtttc ttctgccacg acattttcct
     4081 ttttcaaata gccatgcgtt gcgccatgct acgccgcgcc acactgcgag tatagagtat
     4141 ggtcgagtta aacctgctct ttcaacctta actcacgatt tcttaagcaa aaaatcgttt
     4201 tgctgccgta tatatcgcca ttattcccat ttctgcctgc gaatgcgctg aaaagacatt
     4261 ctctgtttgg tttacacgac aaatatgaat gcgtatattt ctcatttgca tttacaaaca
     4321 aaattattcg cacactaaat ataaccgctc atcaccctcc tccggcatga gcctgacgac
     4381 aacataaacc aagagatttc aaatgctttc aacacaattt aacagggata atcaatatca
     4441 agccagcacc aaaccatcac tacttgccgg ttgcatagca ttggcactat taccttctgc
     4501 cgcttttgcc gctccagcca ctgaagaaac ggtgattgtt gagggttcag ccacagctcc
     4561 agatgatggc gaaaatgatt acagcgtaac gtctacctct gcgggtacca aaatgcagat
     4621 gactcaacgt gatattcctc agtcggtcac tattgttagc cagcagcgga tggaagatca
     4681 gcagttacaa acgctgggcg aagtgatgga aaacacgctg gggatcagca aaagtcaggc
     4741 ggattccgat cgtgctcttt attattcccg cggattccag atcgataact atatggttga
     4801 tggtatcccc acctattttg aatcgcgctg gaatctgggc gacgcacttt ctgatatggc
     4861 actctttgaa cgcgtagaag tagtgcgtgg cgcgacagga ctcatgaccg ggacgggtaa
     4921 tccatctgcg gcaattaata tggttcgaaa acacgcgacc agtcgtgaat ttaaaggcga
     4981 tgtctcggcg gaatacggta gctggaacaa agaacggtat gtggcggatt tacaaagccc
     5041 actcaccgaa gacggtaaaa tccgcgcgcg aattgtcggc ggctaccaga ataacgactc
     5101 atggctggac cgctacaaca gtgaaaagac cttcttctcg ggcattgtcg atgctgattt
     5161 aggcgatctt acgacgcttt cagccggtta cgaatatcag cgcattgatg ttaatagccc
     5221 tacctagggc ggtttaccgc gctggaatac tgatggcagc agcaacagtt acgatcgtgc
     5281 acgcagtacc gcacctgact gggcgtacaa cgataaagag atcaacaagg tctttatgac
     5341 cctgaagcag cggtttgctg atacctggca agcgacactg aatgccaccc actctgaagt
     5401 cgaatttgac agcaaaatga tgtatgtcga tgcctatgta aacaaagcgg atggtatgct
     5461 ggttgggcca tacagtaatt atggacctgg ctttgattat gtcggcggca ccggttggaa
     5521 cagtggcaaa cgtaaagttg atgcgctgga tttgttcgct gacggtattt atgaattgtt
     5581 tggtcgtcag cacaatctaa tgtttggtgg cagttacagc aaacaaaaca atcgttactt
     5641 cagttcatgg gccaacatct tcccggatga aattggcagt ttctacaact ttaatggcaa
     5701 tttcccacaa accgactggt caccacagag cctggcgcag gacgatacca cacatatgaa
     5761 atcgttatat gctgccactc gagtcaccct tgccgatccg ctgcatctga tcctcggcgc
     5821 acgttatacc aactggcggg ttgatacgct gacttacagc atggagaaaa accacaccac
     5881 gccttacgct ggtctggtgt ttgacatcaa tgacaactgg tcgacctacg ccagctatac
     5941 ctctattttc cagccgcaaa atgatcgtga cagttcaggc aaatatctgg ctccaatcac
     6001 cggtaacaac tacgagctgg gtctgaaatc ggactggatg aatagccgtc tgaccaccac
     6061 gttagccatc ttccgtattg agcaggataa tgtcgctcag tccaccggta cacctatccc
     6121 cggcagcaac ggcgaaaccg cctataaagc ggtggatggg acagtcagta aaggggtgga
     6181 atttgaactc aacggcgcaa ttaccgacaa ctggcagctg acatttggcg caacgcgcta
     6241 tattgcagag gataacgaag gaaacgccgt taatcctaat ctgccacgca ccacggttaa
     6301 aatgttcacc agctatcggt tgcctgtcat gccagagttg acagtcggcg gtggtgttaa
     6361 ctggcaaaat cgcgtgtata ccgacaccgt gacaccgtat ggcaccttcc gcgccgagca
     6421 aggtagctac gcgctggtgg atctcttcac ccgctaccag gtgacgaaaa acttctcgtt
     6481 acaggggaac gtcaataacc tgttcgacaa aacctacgat accaacgtgg aaggttctat
     6541 cgtctacggc acaccgcgta atttcagcat taccggcacg tatcaattct gaataacacc
     6601 tgtaaaaaag gcagccatct ggctgcctta gtctccccaa cgtcttacgg attagtggtt
     6661 acggatgtac tcatccatct cggttttcag gttatcggat ttagtaccga aaatcgcctg
     6721 aacaccagaa ccagcaacca ctacgcccgc tgcgcccagt ttcttcaggc cagcctgatc
     6781 cactttagac acatcagcaa cgctgacgcg cagacgggta atacatgcgt cgaggttagt
     6841 aatgttttct ttaccaccaa atgcagcaac cagagccggt gccatttcgc tggtacctgt
     6901 cgcttttgca tcttcagtcg cgtcttcacg acccggcgtt ttcagatcca gtgctttaat
     6961 cagcacgcgg aagatggtgt agtaaacaat cgcataaccg ataccgacga tcgggaacag
     7021 ccacagtttg ctgctgttac cagacagaac gatgaagtcg atcagaccgt gcgagaacga
     7081 cgtaccgtca cgcatcccca gaagaataca gattgggaat gccaggcctg ccagaatcgc
     7141 gtggatgatg tacaggatcg gcgcaacgaa catgaaggag aactcgatcg gctcggtgat
     7201 accggtcagg aacgaggtca gcgccgcgga gatcataata ccgcccactt tcgcgcggtt
     7261 ttctggttta gcagagtgcc agatagcaat tgcggcagct ggcagaccgt acattttgaa
     7321 caggaagcca ccagacagtt tacccgcagt cgggtcaccc gccatataac gcggaatgtc
     7381 gccgtggaaa acctgacctg ctgcgttggt gtattcacca atctgcatct ggaaaggtac
     7441 gttccagatg tggtgcagac caaacggtac caggcaacgt tcgatgaaac cgtaaatgcc
     7501 aaacgcaact accgggttct ggtaagcagc ccactgagag aaggtctgga ttgcagaacc
     7561 aatcggcggc caaatgaagg acagcacaac gccagtaaag atggcagcca ggccagaaat
     7621 gatcggcaca aagcgtttac cggcaaagaa gccaagatac tcaggcagct taatacggta
     7681 gaaacggtta aacatgtacg ctgcgatcgc accggagata atccctccga gtacgccagt
     7741 atccgccagg tgtttagagg cgatttcttc agcaggtaaa tgcagtacca gtggcgcaac
     7801 cacggccatg gttttaacca tgatgccata ggcaacaact gcggccagcg cggatacgcc
     7861 atcgttattg gtaaagccga gggcgacacc gatcgcaaaa atcagtggca tgtttgcaaa
     7921 gacggaaccg cctgcttctg ccataacatg cgatacaacg gcgggcagcc agctgaaatt
     7981 cgcggaaccg acgcccagca ggatacctgc gataggcagt acggataccg gcagcatcag
     8041 cgatttaccg accttttgca ggttagcaaa tgcattctta aacataattg agagtgctcc
     8101 tgagtatggg tgcttttttt acgttctcac gcgtggcaag gggggagagc ctcgccgtgt
     8161 acagggcatc taagcgccct ttatttatta cacagagtaa aataattcag tgccaatttg
     8221 tttgacggct atcacgtttc aacttcagat cattggagaa gttgcacatt ttgacaaaac
     8281 atatgtcaca atatttcaaa cgtggttatt caccgcccaa ttttcggcgg tgaactttac
     8341 tcgttttagc cattaattac gagctttaaa aaaactcatt caacggatgg attgaaggcg
     8401 ggaaacgtcg atgtgaaaca gacgggcgaa gttatcggtg gttacctgcg ccagttcttc
     8461 aacggcaaca cctttcaaca cagccatgta ttctgcaacg tcacgaacca tcgcaggttg
     8521 attctctttt cctcgatgcg gtaccggcgc aaggtaaggt gagtcagttt ccaccagtaa
     8581 tcgatccagg gggacataac gcgcagcatc gcgcagttgc tccgcattac ggaaggtcac
     8641 aatgccggaa aaggagatgt aaaatccgag atccagtaat ttacccgccg tttctctgtc
     8701 ctctgtaaaa cagtgtagta cgccaccgca atccgtcact ttttcttcgc gcagaattgc
     8761 cagcgtatcg gcacgggcgt cacgggtatg gacgataacc ggcttgttca gttcacgacc
     8821 aatctggata tgatggatga aggactcttg ctgacgtact ttagtttccg gtgtgtaata
     8881 ataatccagc ccggtttcac ccagcgctac aacaccctct tctgccgcca gacggcgtaa
     8941 atcttctaca tcgtagggat cattctggtt gagtggatgc acgccacaag aaaatacgac
     9001 gttgtcacgt tcgcctacca gatcccgcat atgtaaataa cccggtaatg ttgtggcgac
     9061 tgccagacaa aatttcacat cgcgtgcggc ggctttcgcc agaacgtcat ccacgtcctt
     9121 atgcaaagat tcataatcca gaccatcgag atggcagtgt gagtcgacta aaaacatgat
     9181 gtctctctta aagatgagga accggtagca caacgcccgg ttgcaggtaa tgctcaatac
     9241 gcagtaaaag atcggtgatg agaagctcgc ggttgatgcc tgtaacagac attaactgtt
     9301 cacgaatgtg gcaaacatcc cccagtatag cctgcaggcg cgagggagaa agatggtttg
     9361 ccagttcggc gaccaggccc ggcacatcaa cattggtcac ctgcgcagca ccatgatggc
     9421 gttttagcgc atccatcagc aacgttgcca gccagtgtaa acgcgccgga gcttgttcat
     9481 gattaagggc cgctagcagc gaataccagt cgcccgatgg cacgctatat gccaacgcct
     9541 gacacaatgt ttcacgagcc tgccagttat ctccctgaaa caacgccagt gccgcgccag
     9601 gcgaaccggc gcttaagcgc aatgcggcaa gtaatgcatc ctgtgacatt gtcacttcgc
     9661 gtgaaagcca ggtcacggcg tactgttccg gcggcggcgc aaggtaatgt aaccgacaac
     9721 gactacgtaa tgttgccagt aaacgttcag gctcgcgggt agccaggaaa aaccaggttt
     9781 ctgctggtgg ctcttcaagc gttttcagca atgcgttagc cgcggcgtcg gtcagtaagg
     9841 cagcatcggt tacccagaca actttcgcac cacctaggcg tgcgtgctca ttcagctttt
     9901 cggtgacctc acgtaccgca tcaatgccca gcatattttt ccctttttcg ggagccaggg
     9961 tgtagtaatc gggatgcgtg ccagcctgca tcaactgaca tccacgacag tgaccgcaac
    10021 ttttgtggcc ctgcggttgt tggcagagta aataacggct cagggcgtag attaaagcat
    10081 catcgcccat gcccggtaac gcctgaatga gtagcgcatg gtgacctctt ccggcctgat
    10141 agctggctac cagtttttcg aaatcaggtc gtaaccatgg ataccatctc atgcgtccaa
    10201 ctccttaacc cagtgggtca cggtagtgcg gattgcatcc atcacggcct ccagcggctg
    10261 ggtggcatca atggtatgaa tacttttatc ttgtgcagcc agttccagat agcgggcgcg
    10321 ggtgcgatta aagaaatcga aagactcttg ctcaatacga tccagctcgc cgcgcgcacg
    10381 cgcgcgtttt aagccaactt ccggggtaac atcgagatag agcgttaagt cggggcgaaa
    10441 atccccgaga acagcatcac gcagtgttgc cagcatatgt tggtcaatac cacgtcctcc
    10501 gccctgatac gcctgagtgg agagatcgtg gcgatcgcca atcacccagg tgccgttagc
    10561 cagagctggt ttgatgaccg tttctaccag ttgaacgcgc gcggcataaa acatcagaac
    10621 ttcggcttta tcggtaatga cttcatcgcc taccgatttg atatccagca ccaggcttct
    10681 taacttttcg gcaagttgcg taccgccagg ttcccgagtg aaaaccatgt cgcggatacc
    10741 cagttgctca agcgtctcaa ccaccacatt acgcgcggta gttttgcctg cgccttccag
    10801 cccctcaatg acgatatact tactgcgcat ttttttcctt aagcactttc agataatcct
    10861 gcacagactt gttatgactg gcaagattgg tattaaacgt gtgaccacct ttaccatcgg
    10921 ccacaaaata gagatacggc gtttttgccg gatgcgcagc agccttcagc gaatccgccc
    10981 ccggcgtagc tatcgcaccc ggcggcaaac cggtaatggt ataggtgtta tacgctgtcg
    11041 gcgtttccag gtctgcacga gaaagtttgc cattataacg ctctcccatc ccgtaaatca
    11101 cggttgggtc ggtctgcagg cgcataccaa tgcgtaaacg gttgataaat accgaggcaa
    11161 cctgatcgcg ttcactggca acggcggttt ctttttcgat aattgatgcc atcgtcacca
    11221 gctgattttt atctttataa ggcagaccgt ccgcacgccc ttcccaggcg ctatcgaccg
    11281 ctttcaccat tttcttgtgc gcttgcttga gtaacgcgac atcggtggta ttggcggtat
    11341 acatccaggt gtctggccag aaccaacctt caatccactc cgggttttcc agttcaagtg
    11401 cctgcgctac ggtggcgtac ttatcgtcac tcagcgtgtg cttgatatac ggagcctcac
    11461 gcaattgctt gaggtagtcg ctcagacgca tcccttctac cagtcgcagc gggaactgtg
    11521 cttctttacc gctttccaac aatttcagca tctcgcgcac ggtcatctgc ggtgtaaagc
    11581 ggtaagtccc ggctttaaag tgagaaagat ccggttcgat acgcagcagc cattgaaaaa
    11641 cccgtgggcg attgatgatc ttatcagcat aaagctgttc accgagcgcc agacgtccgg
    11701 tccctggctt cagggtaaat atcgtctctt ctttgataag caatttgctg tcggcaagat
    11761 ggcgaacctt ccagacgccc acaccagcgg cgatacccag taccaccagc aataacaaga
    11821 ttatcaataa cacttttttc atgactaatt cgggcgctca caaagtgggg ctaaatattc
    11881 atataacgtt gctgacgaaa aggagacatc gccacaggca cgtacgggca tcactggcat
    11941 taacgcatta caaataacca tctcatctgc ctgcaacgcc tcttccagag aagcttgcac
    12001 ttcgacaagc tgataagagg attgtgccag caaacggata cagaattgtc gcataatgcc
    12061 gttaacacct gcctgatcca gtcgcggcgt ataaactacg ttgcccttcc gccagaacaa
    12121 attagccgca cagcattccg taacccaccc ttcgctgtca aggaccagcg cctcatcagc
    12181 gtttgtctgc tcaagatgag agcgaatcaa tacttgctca agacgattga gatgtttaat
    12241 acctgcaaga tgaggattgc gccccagccg caccgggctt agcgccaacg tcatcccctc
    12301 gttacgcaaa cggtcgtaat gtgcaggata agccgtaacg gagagaatcc gcgttgccgg
    12361 tccactgttc aatgtgctgt accctcgccc gccactaccg cgactgatca cgactttcag
    12421 tacaccattt tgctgttctg ctgccagcgt tttcatctct tgttcaagct gtggccagaa
    12481 gtcacaggaa atcatcaacc gctgacaagc atcctgtagt cgctggatat gcgccgataa
    12541 caaactgact ttaccgtcga taactctggc ggtggtaaaa caaccatcac caaactgcgt
    12601 tgcccgatcg cttactgcca gcgattcctg cttataaccg ttaattaaga acataccggc
    12661 tccttatcag agaattgtta gtgtggcagc atgttcacta cggaacaagt cggaataaaa
    12721 gctaagaaaa aaggcccgca agcggacctt ttatatgggt ggaaaatgac aacttagatc
    12781 tttttaaaga tcaaagaacc attagtgcca ccgaagccga aggagttaca cagcgtgtat
    12841 tccattccgc tgacctgacg tgcttcatgc ggtacgaaat ccagatcgca accttcatcc
    12901 gggttatcca ggttgatggt tggcggaaca gcctgatcgc gcagcgccag gatggagtag
    12961 atagattcta ctgcacccgc cgcacctaac aggtgaccgg tcatagattt cgtggagctt
    13021 accaacacac ggcttgcagc ttcaccgaag atggttttca ccgcctgcgc ttcagcttta
    13081 tcgccagccg gcgtagaagt accgtgcgcg ttaacgtagc caatctgact cgcttcaata
    13141 cctgcatcac gcagagcatt tgccattgcc agagctgcgc ctgcgccatt ttctggcggt
    13201 gacgtcatat gataagcatc gctgctcata ccaaagccga cgagttcagc gtaaattttc
    13261 gcaccgcgtt ttttcgcgtg ttcgtactct tcaagtacca gcataccggc accatcgccc
    13321 agtacgaagc catcacgctc tttatcccac gggcggctcg ccgcttgcgg gttatcattg
    13381 cgggtagata atgcacgtgc cgcgccaaaa ccaccaacac ccagcggcgt actggctttc
    13441 tctgcgccac ctgcaaccat cacgtcagca tcgccatacg cgataatacg cgcagcatga
    13501 ccaatgttgt gcacgccgga agtacaggcg gtcgcgatag agatgcttgg gccacgcagg
    13561 ccatacatga tagtcagatg acctgccacc atgttcacaa tcgttgacgg aacgaagaat
    13621 gggctgatct tacgtggacc accgttcatc agagatgtgt ggttttcttc gatcagtccg
    13681 aggccgccaa tcccggagcc aattgcggca ccaatgcggg ttgcgttctc ttccgttatt
    13741 tcaaggccag aatcctgcat ggcctgaacg ccagcgacaa ttccatattg aatgaaggca
    13801 tccatcttgc gctgttcttt gcgcgagata atgtcctcac agttaaaatc ctttactaag
    13861 ccagcaaatt tcgttgcata ggcgctagta tcgaaatggt cgattaggct gatgccactc
    13921 tgaccggcaa gcagagcttt ccaggtagac tctacggtat tgccgacagg agacaacatg
    13981 cccagtccgg tcacaactac acgacgctta gacacgtttg tcctccaggg agggaaaaaa
    14041 tgattctagt gggacaaaaa gataaaactc aggcggtcga acgaccgcct ggagatgttc
    14101 acttacgcct ggtggccgtt gatgtaatca atggcagcct gaacggtggt gattttctca
    14161 gcttcttcgt ccggaatctc agtatcaaac tcttcttcca gagccattac cagctcaacg
    14221 gtgtcaagag aatccgcgcc caggtcttca acgaaagaag cattgttggt aacttcttcc
    14281 tgcttaacgc ccagctgttc gccgataatt ttcttaacgc gttcttcgat agtgctcata
    14341 ctcttaaatt tcctatcaaa actcgctttc gcgatggttt tcgtagtgta taaaatgttg
    14401 aaaaatttgc aactaaatcc cggcaggtct taccacgatt ttacgttatt ttgaggcctt
    14461 accccccaat aacgcaaatc attttgcact aattattact cataaccacg cagactgcgc
    14521 gcaacatgag cattttgtgc aaatcgcggt cagaccatgt acatcccgcc gttcacatgc
    14581 aaagtttcac ccgtgatgta agctgcttcg tcggatgcca ggaatgcaac cgcgttggcg
    14641 atttcctgtg cgccgccgag gcgacccgca ggaacctgcg ccaggatacc cgcacgctgg
    14701 tcatcgctca gcgcacgtgt catgtccgtt tcaataaagc ccggagcaac aacgtttaca
    14761 gtaataccgc gtgacgcaac ttcgcgcgcc agtgatttac tgaagccgat caagcccgct
    14821 ttcgccgcag cgtagttggc ctgaccgcca tttcccatgg taccaaccac agaaccgata
    14881 gtgataatac gaccatgacg ctttttcatc atagcgcgca ttaccgcttt tgacagacgg
    14941 aaaacagatg aaaggttggt ttcgataata tcgttccact cttcatcttt cattcgcatt
    15001 aacaggttat cacgagtgat accggcatta ttgaccagga tatccacttc accaaattct
    15061 gcgcgaattt tttccagaac agattcgata gatgccgggt cggtcacatt caacatcaga
    15121 cctttgccgt tagcacctaa ataatcactg atcgcctgag cgccattttc actggtcgca
    15181 gtgccaataa ctttcgcgcc acgggctgcg agcgtttcag caattgcgcg gccaattccg
    15241 cggcttgcac cggttaccag tgcgattttt ccttcaaaat tcatgatttt cctcttttaa
    15301 agctcgagcg ccgctgccat cgctgaaggt tcgttcagcg ccgaggcggt cagggtgtcg
    15361 acaatgcgtt tcgtcaggcc agttagcact ttgcccgggc cgacttcata gagatgttct
    15421 acgccttgcg ctgccatgta ctcaacagac ttcgtccact gaaccgggtt atacaactga
    15481 cgtaccagtg cgtcacgaat ggcatcacca ttggtttcgc atttcacatc aacgttattc
    15541 acaacaggaa ctgttggtgc gttaaaggtg attttcgcta attctactgc cagtttgtcg
    15601 gctgctggtt tcatcagcgc acagtgagac ggtacgctca ctggtaacgg cagagcgcgt
    15661 tttgcgcccg cagctttaca ggcagcgcca gcacgctcaa ccgcttcttt atgaccggca
    15721 ataaccacct gtcccggaga gttaaagttt accggagaaa cgacctgacc ttctgcggct
    15781 tcttcacacg ctttcgcaat agacgcatca tccagaccga tgattgccgc catagcgccc
    15841 gtgccttctg gtacggcttc ttgcatgaac ttgccgcgca tctcaaccag acgcaccgca
    15901 tcagcgaaat caatcacgcc agcgcaaacc agcgcggagt attcccccag gctgtgaccg
    15961 gccatcattg ccggtgcttt accgccctgc tgctgccata cgcgatacag cgcaacagat
    16021 gcagtcaaca gcgctggctg ggtttgccag gttttattca gttcttcagc tggcccctgc
    16081 tgggtcagcg cccacaggtc gtagcccagc gccgcagaag cttcagcaaa cgtttcttcg
    16141 acaattggat agctcgccgc catatcagcc agcattccaa cggtttgaga accctgtcca
    16201 gggaacacaa atgcaaattg cgtcatgttt taatccttat cctagaaacg aaccagcgcg
    16261 gagccccagg tgaatccacc gccaaaggct tcaagcagaa ccaactgccc cggcttaatg
    16321 cgcccgtcgc gtacagcttc atccagcgcg cacgggacag aggccgcaga ggtattaccg
    16381 tggcgatcca gcgtcaccac gacattgtcc atcgacatac cgagtttttt cgccgttgca
    16441 ctgataatac gcaggttagc ctgatgcgga accagccagt ccagttgaga acggtcaaga
    16501 ttattcgccg ccagcgtctc atcaacgatg tgcgccagtt ccgttaccgc aaccttgaag
    16561 acttcgttgc ccgccattgt cagatgaatt gaattctctg gattcacgcg gtcggcgttt
    16621 ggcagcgtca gcaattcacc ataactaccg tcggcatgca gatgggtgga gatgattccc
    16681 ggctcttcag aggcagccag caccgcagcg cccgcgccat cgccaaaaat aataatagtc
    16741 ccacgatctg ttggatcgca ggtgcgcgcc agtacatcgg aaccgacgac cagagcatac
    16801 ttcaccgccc cagatttcac gtattgatcg gctacgctta atgcataggt gaaacctgcg
    16861 caggctgctg caacgtcaaa tgccgggcaa cctttaatgc ccaacatgct ttgaatctga
    16921 caagctgcgc tcgggaaagc gtgcgtagca gaagtcgttg ccacaacgat caggccaatc
    16981 tggtctttct caatgcccgc catctcaatt gcgcgtgtcg ccgcttcaaa gcccatggtt
    17041 gaaacggttt cgtttggcgc ggcaatgtgg cgttcgcgga taccggtacg agtgacaatc
    17101 cactcgtcag aggtgtccac cattttttcc aaatcggcgt ttgtccgcac ttgttcgggc
    17161 agatagctgc cagtaccaat aatcttcgta tacatgtacg ctcagtcact tttcggttat
    17221 ataccgtcac ttgcaaactg cgagttcgct ggcagcgtcc tgctaccgca gagttccgct
    17281 tttgccaccg tccagcagct caaaaccagc tgggtataca gattccaggc gagcggcaat
    17341 tcgctgagga acttgtcgct gcaccgcctg cactgcctgt tcaatcgcga ccgcaaaagc
    17401 tcgctgattg gctgcaccat gactttttat caccgtgccg cgcaatccta acagacaggc
    17461 gccgttatac tggtcggggt tgaggtgact gaatcgcctc gtcaggctct tttgtagcca
    17521 acgctttaat aacagtagcc accacgaccg ttttttccct tcaccctgag atttcagcag
    17581 agaaaggaac atcctgacaa caccttccat cgtctttaac gtgacatttc ctgtaaagcc
    17641 gtcacaaacc agcacatctg tcttgccagt taacaactca ttggcttcaa gatagccgat
    17701 ataattgata gaagggattg ttttaagcac cgctgaggca tcccgaatac tgtcgagacc
    17761 ctttacttct tcttcaccaa tattgagcaa cgccacgcga ggattgggaa tttccaccac
    17821 ctcttcagcc agaactgagc ccataatggc aaattgcacc aacattgtgc tgtcacaatc
    17881 gacgttggcc cctaaatcga ggaccaccgt tttgcccttt tgctgatgtg gtaataccgt
    17941 caccagcgcc ggacgctcaa tcccctccag gggcttgagt aataattttg ccagccccat
    18001 cagtgccccg gtattaccgg cactgacaca ggcttgcgct cgaccttctt tcaccagctc
    18061 cagggccata cgcattgaac tcccacgact ggcgcggata gcttgcgaag gccgggcatc
    18121 actggcgata actgactgcg caggaataat ctgcagacgc gaacgttgtt caaagtcagc
    18181 tttagcaagt aatggcgtga tggcgtcggg attgccgact aaaagaagag tgagttgcga
    18241 attagaattc agtgcctgca atgctgcagg cactgtcacg gaagggccaa aatcccctcc
    18301 catgacatct aacgccaggg ttagacgtgt caaggtatcg tcgccgcccg gtttggtctt
    18361 tccccagttg cctggggaaa atcctcacta agcttcatca cgcagacgcg tgattactta
    18421 gcgatgacct tgcggccgcg gtagtaaccg tcggcagtga tgtggtgacg caggtgtttt
    18481 tcaccagaag ttttgtctac agacaggctg gtgactgcgg tcagcgcgtc atgggaacga
    18541 cgcatgccac gtttggaacg ggttggttta ttctgttgta cggccatgga ccttactcct
    18601 caattacggt ttatccccga tccaacgggg agcaccaatt acttacgctt taagctggct
    18661 aatacggcaa atgggtttgg cttttgcgct tcttcaggca gttcaccaaa gaccatgtcc
    18721 gcttcggaca cttcacagtg ttcagaatca tgcaccggaa ctaccggcaa ggcgaggatg
    18781 atttcatctt caaccattgc aagcagatcg atttcaccga attcgttaac ctcaatcggt
    18841 tcatacgctt ccggcagtgc ttcagcctgt tcgtctgaac gcacaggact aaaacaatac
    18901 gttgtgtaga cctgatgagt aaacggcttc ccgcaacgct gacactcgag cgttaccgtc
    18961 accttcgcat cgccgtttaa cactgcgaga cgttggttat cgatagcgaa cgacatggag
    19021 cattccacat cactgtccac actgactacg gattcggcga cgcgctcaac ctgatcaggg
    19081 gtatagatac cctggtaatc aaggcgtttt tgagccgtac gaaccggatc gagagtcagg
    19141 ggtaatttta ccttttgcat agggcgcgca tattaacttt gtaacgtcat agagtcaaag
    19201 aaaaaggcag cctgtggttg ccttttgcca ataattcgca cacattgcgg gttactgctt
    19261 tattttcgtt cagtgctgat aacgaacatc aacctgcaaa acgaagcgca tagtttaaaa
    19321 tggctatcat caatacgcta tatctgatgg aaaaaaatat gcctaaactt attttagcct
    19381 ccacttcgcc ctggcgtcgc gctcttctgg aaaaactgca aatctctttt gaatgtgcag
    19441 caccagaggt cgacgaaacc ccacgcagcg acgaatcacc gcgacagttg gtgcttcgac
    19501 tggcacaaga aaaagcacaa tctctggcgt cacgttatcc ggatcattta attattggtt
    19561 cagaccaggt atgtgttctt gatggcgaaa tcaccggaaa accgttaacg gaagaaaatg
    19621 cccgtctgca attacgcaaa gccagcggca atatcgtcac cttctatact ggactggcgt
    19681 tgtttaattc ggcgaatggg catctgcaaa cagaagtgga gccttttgac gtccatttcc
    19741 gtcacctgag cgaggcggag attgataatt acgtgcgtaa ggagcatccc ctgcactgcg
    19801 cgggtagctt taagagtgaa ggatttggca ttacgctgtt tgagcgttta gaggggcgcg
    19861 accctaacac actggttggt ttaccgctaa ttgcgctgtg tcagatgtta cgtcgggaag
    19921 ggaaaaatcc gctgatggga tagttttttg cgccggatgc ggttacgtcg catccggcaa
    19981 tcggtgcagg cggcctgatg cggcgtaaac gccttatcag gccaaccgcc cattatcagg
    20041 cttatatgct tgtattagcg cgcgttacgc agcttttgca aacaacgctt caaaccttca
    20101 tccatcggcg cttcgatgcg catcacctca ccggtccccg gatgggtaaa cttcaacgct
    20161 gcggcgtgca ggaacagacg atttaatccc gtgcctgctt cagtgagctg tctgtcaaat
    20221 tcacggtcac cgtagcgatc gtcaaaggca atcggatgac ccgcatactg tgtatgcaca
    20281 cggatctgat gagtacgtcc ggttaccggg ctacaacgca ccagggtggc aaatgcatag
    20341 cgttcttcca ctttaaagcg tgtttccgac ggtttgcctt cctgactgac acgcacgata
    20401 cgttcgccgc tttgcagaat atttttcaat aacggcgctt gaacgctctt cacatgcgac
    20461 tgccactgac cgcgcaccag cgccaggtaa tctttttgca ttcctttttc acgtaattgc
    20521 tcatgcagag aacgcaacgc cgagcgtttt ttcgctacca gcaacacacc tgaagtgtcc
    20581 cggtcgagac gatgaaccag ttcaaggaac cgcgcttccg ggcgcaacgc ccgcaaacct
    20641 tcgataacgc cgaagcttaa accactgccg ccatgtaccg ccgtaccgga aggtttattc
    20701 agcaccagga tatgatcatc ttcatataag atgacgtccg ccagcgccgc caccttttgc
    20761 agatgtggcg aaaccgcctc ttcttcccgc tcagcaacgc gaaccggtgg gatacgcacc
    20821 tcatcacccg cttcgagttt atattcaggc ttaatacgtt ttttgttcac ccgcacttca
    20881 cctttacgca aaatacggta aatcatactt tttggtacgc ctttcaattg ggtacgcaaa
    20941 aagttatcga tacgttgccc cgcttcgtca gcggtgatag caacaatttt tacggatgga
    21001 gtctctgttt tcatggtggg cgattctaat tagccaacag gattcgcgcc actcattttt
    21061 ctatgcttat atttagtttt gcaccttatt acttcactac gtgatcactt tattgatggt
    21121 tattaaacca atcaccagca agaagtgaaa aaactgtgag taagcgggtg ataaatggta
    21181 aaagtcatct tgctataaca aggcttgcag tggaataatg aggccgtttc cgtgtccatc
    21241 cttgttaaaa caagaaattt tacggaataa cccattttgc ccgaccgatc atccacgcag
    21301 caatggcgta agacgtattg atctttcagg cagttagcgg gctgcgggtt gcagtcctta
    21361 ccggtagatg gaaatatttc tggagagtaa tacccagtct gtttctttga taattgcgct
    21421 gtttttccgc atgaaaaacg ggcaaccgac actctgcgcc tctttgagct gacgataacc
    21481 gtgaggttgg cgacgcgact agacacgagg ccatcggttc acacccggaa aggcgttact
    21541 ttgcccgcag cttagtcgtc aatgtaagaa taatgagtaa gttacgatga aaagaatgtt
    21601 aatcaacgca actcagcagg aagagttgcg cgttgccctt gtagatgggc agcgtctgta
    21661 tgacctggat atcgaaagtc cagggcacga gcagaaaaag gcaaacatct acaaaggtaa
    21721 aatcacccgc attgaaccga gtctggaagc tgcttttgtt gattacggcg ctgaacgtca
    21781 cggtttcctc ccactaaaag aaattgcccg cgaatatttc cctgctaact acagtgctca
    21841 tggtcgtccc aacattaaag atgtgttgcg tgaaggtcag gaagtcattg ttcagatcga
    21901 taaagaagag cgcggcaaca aaggcgcggc attaaccacc tttatcagtc tggcgggtag
    21961 ctatctggtt ctgatgccga acaacccgcg cgcgggtggc atttctcgcc gtatcgaagg
    22021 cgacgaccgt accgaattaa aagaagcact ggcgagcctt gaactgccgg aaggcatggg
    22081 gcttatcgtg cgcaccgctg gcgtcggcaa atctgctgag gcgctgcaat gggatttaag
    22141 cttccgtctg aaacactggg aagccatcaa aaaagccgct gaaagccgcc cggctccgtt
    22201 cctgattcat caggagagca acgtaatcgt tcgcgcattc cgcgattact tacgtcagga
    22261 catcggcgaa atccttatcg ataacccgaa agtgctcgaa ctggcacgtc agcatatcgc
    22321 tgcattaggt cgcccggatt tcagcagcaa aatcaaactg tacaccggcg agatcccgct
    22381 gttcagccac taccagatcg agtcgcagat cgagtccgcc ttccagcgtg aagtccgtct
    22441 gccgtccggt ggttccattg ttatcgacag caccgaagcg ttaaccgcca tcgacatcaa
    22501 ctccgcacgc gcgacccgcg gcggcgatat cgaagaaact gcgtttaaca ccaacctcga
    22561 agctgccgat gagattgctc gtcagctacg cctgcgtgac ctcggcggcc tgattgttat
    22621 cgacttcatc gacatgacgc ctgtacgcca ccagcgtgcg gtagaaaacc gtctgcgtga
    22681 agcggtgcgt caggaccgtg cgcgtattca aatcagccat atttctcgct ttggcctgct
    22741 ggaaatgtct cgtcagcgcc tgagcccatc actgggtgaa tccagtcatc acgtttgtcc
    22801 gcgttgttct ggtactggca ccgtgcgtga caacgaatcg ctgtcgctct ctattctgcg
    22861 tctgatcgaa gaagaagcgc tgaaagagaa cacccaggaa gttcacgcca ttgttcctgt
    22921 gccaatcgct tcttacctgc tgaatgaaaa acgttctgcg gtaaatgcca ttgaaactcg
    22981 tcaggacggt gtgcgctgtg taattgtgcc aaacgatcag atggaaaccc cgcactacca
    23041 cgtgctgcgc gtgcgtaaag gggaagaaac cccaacctta agctacatgc tgccgaagct
    23101 gcatgaagaa gcgatggcgc tgccgtctga agaagagttc gctgaacgta agcgtccgga
    23161 acaacctgcg ctggcaacct ttgccatgcc ggatgtgccg ccagcgccaa cgccagctga
    23221 acctgccgcg cctgttgtag ccccagcacc gaaagctgca ccggcaacac cagcaactcc
    23281 tgcacaacct gggctgttga gccgcttctt cggcgcactg aaagcgctgt tcagcggtag
    23341 tgaagaaacc aaaccgaccg agcaaccagc accgaaagca gaagcgaaac cggaacgtca
    23401 acaggatcgt cgcaagcctc gtcagaacaa ccgccgtgac cgtaatgagc gccgcgacac
    23461 ccgtagtgaa cgtactgaag gcagcgataa tcgcgaagaa aaccgtcgta atcgtcgcca
    23521 ggcacagcag cagactgccg agacgcgtga gagccgtcag caagctgagg taacggaaaa
    23581 agcacgtacc accgacgagc agcaagcgcc gcgtcgtgaa cgtagccgcc gccgtaacga
    23641 tgataaacgt caggcgcaac aagaagcgaa ggcgctgaat gttgaagagc aatctgttca
    23701 ggaaaccgaa caggaagaac gtgtacgtcc ggttcagccg cgtcgtaaac agcgtcagct
    23761 caatcagaaa gtgcgttacg agcaaagcgt agccgaagaa gcggtagtcg caccggtggt
    23821 tgaagaaact gccgctgccg aaccaattgt tcaggaagct ccagctccac gcacagaact
    23881 ggtgaaagtc ccgctgccag tcgtagcgca aactgcacca gaacagcaag aagagaacaa
    23941 tgctgataac cgtgacaacg gtggcatgcc gcgtcgttct cgccgctcgc ctcgtcacct
    24001 gcgcgtgagt ggtcagcgtc gtcgtcgcta tcgtgacgag cgttatccaa cccagtcgcc
    24061 aatgccgttg accgtagcgt gcgcgtctcc ggaactggcc tctggcaaag tctggatccg
    24121 ctatccaatt gtacgtccgc aagatgtaca agttgaagag cagcgcgaac aggaagaagt
    24181 acaagtgcag ccgatggtga ctgaggtccc tgtcgccgcc gctgtcgaac ctgttgttag
    24241 cgcgccagtt gttgaagaaa tggctgaagt cgtagaagcc cccgtaccgg ttgccgaacc
    24301 gcaaccggaa gtggttgaaa cgacgcatcc tgaagtgatt gccgccgcgg taactgaaca
    24361 gccgcaggtg attaccgagt ctgatgttgc cgtagcccag gaagtcgcag aacacgcaga
    24421 accggtggtt gaaccgcagg aagagacggc agatattgaa gaagttgccg aaactgctga
    24481 ggttgtggtt gctgaacctg aagttgttgc tcaacctgcc gcgccagtcg tcgctgaagt
    24541 cgcagcagaa gttgaaacgg tagccgcggt tgaaccagag atcaccgttg agcataacca
    24601 cgctaccgcg ccaatgacgc gcgctccggc accggaatat gttccggagg caccgcgtca
    24661 cagtgactgg cagcgcccta cttttgcctt cgaaggtaaa ggtgccgcag gtggtcatac
    24721 ggcaacgcat catgcctctg ccgctcctgc gcgtccgcaa cctgttgagt aataattagc
    24781 tcaaagtaat caagccctgg taactgccag ggctttttta tttcatcttt gaatcaacat
    24841 attaaaattc atcaaatggc atccttgcta accaacaatg caaaataggc aaaaaaaatg
    24901 cccgccccgg cgatcccaga gcaggcagac aaaacatacc cagtttcatg atatgtttta
    24961 aagcgagctt atttgttgag ctggaagagc gacaatccct gcatatcggt aaatgcttta
    25021 tacgatgcct gcaatgccgt ttgctgcatg atgtaagatg aaatagttgc attccagtcc
    25081 acatcaacca gatcgctcat ctgctgcgtt tgccctaaag cgcgatcgct acctaaggaa
    25141 tccagcgact ccagttcgtt cagctgcgtg cctaattccg cgcgaacagt cagcacattg
    25201 ttcagcgagt ttttcagtcc gcggttggtt ttatctaacg ccgcagcggc ggtttcttta
    25261 tccgcttcgc tatccgcgac cggcgttttc agggctgcga tggcactatc cagcatggca
    25321 aaaagattgg tttcagaagc gctaccgtct ggttccgcta ccgcgttgct ggtaatactg
    25381 tcgaaaattt tgtcacccgt gtgccctatc accatcgaac gcgaagcatc gacctgttgt
    25441 ttaatacttt ctgctccacc gacgtatttc cctttctctt cgctaaacgg cgcagtctct
    25501 gttttataac cggcaaaaat gtagcgcccg ttaccgtcag tggtgttcgc cagattcagc
    25561 aactggtcac gaagcccctg aatatccgta gccagcgagg cccggtcatc gtcactcaag
    25621 gtgccattgc tggcgtagac aattttttcc tgagcattct ggatagcagt ggtgacctgg
    25681 ctaagtacac tctcttccag tgacactttt tgagtggcga aagtacgtgc cagcgtgtac
    25741 tggctgtttt gcgcctgtgc ctgggagaga acaacagctt gtgatgcagc aatgggatcg
    25801 tctgaagggt taacgactcg cttacccgtc gacatctgct cgccgtactt catccattct
    25861 gcctgagaat tggtgatgcc gcgcatgttt tgctggtaca tcatctgggt actgaaacgc
    25921 attttatctc cccttagcga atgttaatca gcgcatcaaa aatcgcgttt gccgtctgca
    25981 gaacctgcgc attcgccagg taatactgct gaaaacgttg cagatttccg tactcctcat
    26041 cgagattgac accggaaatc gactgctgct gattggaaag ctgcgtcacc acattacctt
    26101 gcgtggtgct actggttttc aacgtcgcgg ttttattacc gatatcactc actaacgagg
    26161 cataagcgtc gttaaaggat ttcgcaccac ccaccgtttt actgttgctt tgcagatcca
    26221 gcagggcctg accgttgcgg ttgtcgctat cacccgcatc ttcttcgctc gccatcgcga
    26281 ttttcgcttc gtcggtgatt aatacatcca tgttgacgat ggcgtcactt actggtttca
    26341 gcgtgaagct gtcgttaacg gcaggcgttc ctgtaaacgt caactccaga ccatcaaatg
    26401 ccactttacc gttggcatct ggtgtcgccg taaaagtggt attgctggca aggcgggtga
    26461 cctgccactg attattatcg aacgagattt tgtaatctgt cgccagtacc acggaggcat
    26521 cagttaccgt ggcaccgatc gcaacgtcac ctttgttttt cgtgttttgc agaaccgcgg
    26581 gcttaccgat agtaaagaaa tcttcaccag catcgccgtt agcatcaaat ccggctttgt
    26641 gttggctgtt gaaagcctcg gcaaatgcca gcgccagttg tccaagcgta ttacgcgtct
    26701 ggtccagatc ctgagaacgg aatgtcagaa tgccgcccag cgacccggta ttcagtaatt
    26761 tctccgggat ctcaatattg cctgccgtcc catcaacgta agcgacagtc gtacgagaag
    26821 ggtcggcgct ggaaggaact gccgccagtt gccgcgccgt acttccctga accagtgagt
    26881 aaccattggc catcgtgatg ttataagtgc cgccatcctg aacgctgact tctacaccaa
    26941 caatctggtt taattcgctc accagttgat cgcgttgatc cagcagattg ttaggtgacg
    27001 cccctgcccc cacgcctgtc aggcgggaga tttgatcgtt caggctggcg atttgtttag
    27061 cgtaattgtt gatctgatca acgctggcac ctatcgcgat attgacctgt ttgtcctggt
    27121 cgcgcagata ttgatcggtg gttttaaact gattcaccaa cccttctgat ttcccaatca
    27181 gcgcctggcg cgctgccggg tcttccgcgt tactcaccag cgtttgcagg ctggtgaaga
    27241 aatcctgcat ctgtgttgcc agcgaagagg tactggtgga gagcatattg tcgattttcg
    27301 acatctgctc atagcgggca gtcaggccgc tactttgcgt ctgcgccgca cgtaactggt
    27361 tggtaataaa agcatcatac tcacgctgca caccagaaac gtagacacca ttgccaaccc
    27421 agccgccagc gcccaacgtg ctattggcct gcgccataat agtggtttgg cgggtatatc
    27481 cggcaacgtt atagctggag atattattac ttgccgtatt taacgccgcc tgggccgcgt
    27541 tcagtccgct catggcgtta ttaatcaagc tggacatgga ggttccttat aagccttcaa
    27601 ttacagagta ttatcggcag cgacccgccg gacttgagtt attcagaaca gattatcaat
    27661 gttcatgctg taggttttgc tcaccttgtc gcttatcgat ttcatctgct gaatcatgtt
    27721 ggtgagtttg cgggcatagt gaggatcggt ggcatagccc gcgtcctgta gggcctgcgc
    27781 cccctgttcc gcactcgcgg cggtcgtcac ggcggcgtag cgcgggttac gcgttaacag
    27841 cccaacgtaa tccgacaagg cttccagata cgagctgtag acgcgaaact tcgcttttac
    27901 tttcttcgct tcgccgtttt catattcagt cgtggtgatt tcagtaactg gccctttcca
    27961 gttgccagag gctttgacac caaacaggtt atagctcggc tcgccgtttt cgcggcggat
    28021 ttgccgttgc ccccaaccag attccagtgc cgcctgagcg aggatcaaat gatgtggcac
    28081 accgctttgc tggcttgcca gttgggcggg cagcgagagt tgcgcgagga atgctttact
    28141 gtcacccggc agcgaatcat cgtagttacg tggcactgcc ttttgcacca gctgcgaaag
    28201 cgcctgattt tgataacgca ccacggtttc gagcgggaat ttcatcggta ctgctggcgt
    28261 ggactcctct ggcaatggtt gttctggcgt catctgttta accatcatct ctgcaagccc
    28321 cagacctttg cccgccgtca tctgttgggc aatttgctgg tcatacatac tggtatacag
    28381 acgagtgtgc tcgctgctga acaggccatc ttttggtaaa gcgtcgcgca tgcttttcaa
    28441 catcatctgc acgaacatcc cttccacctg gcgggccacc ggacggatat ttgccgccgg
    28501 atcttcgccc gctttcgcct ttagttcgtt gagtgattgc gcatcccagg ccgcacttgc
    28561 cagtagtttg ctgtcgctga tcatcagatg atttccagtt ttgcccgcag acatcccgca
    28621 ctttgcattg attgcaaaat agacatcaga tccatcggcg tagcgcccag cgcattgagc
    28681 gcgcgcacca cgttattgag gctggcgctg gaacgtacgc tttgcagcga accgccgctc
    28741 tggcgtaaat cgatctgcgt ttgtggagta accacagtct gtccaccacc aaacggtgta
    28801 tctggctggc tgacattggc ctgacgattg actgttactg agagatttcc ctgcgctacc
    28861 gcgcagctgt cgagggtcac ttcgcgattc atcaccaccg aaccggtgcg cgagttaatc
    28921 actactttag cgtcctgcgg ggtgacatta acctgcatat tctggatatc tgcaaggaaa
    28981 cggacctggg aactgttgcc acttggtacg cgcacctgaa tagtccgcgc atctaacgcg
    29041 gtggcgctgc catatccacg cacgcgattg atggtgtcag cgatttgctg cgccatgctg
    29101 aaatcttcgt cgttaagttg caaattaagg gtattcccga cgccaaactg gctgggcaat
    29161 tcacgttcaa taaccgcacc attggtgatc cgtccaccgt tcagttggtt cacctgaaca
    29221 ctgctaccgc cagcggaggc tcctgcgccg ccaaccagaa tattgccctg cgccagcgca
    29281 tacacctgac tgtcaacgcc cttaagcggc gtcatcaaca acgtacctcc acgcaagctt
    29341 ttggcatttc ccatggaaga aaccaccaca tcgatggttt gcccctgacg tccaaacgga
    29401 ggaagtgacg ctgtcaccat taccgcagcg acgtttttta gctgcatatt ggtgcccgtc
    29461 ggaacggtaa ttcccagctg tgagagcatg ttattaagcg tttgtgtggt aaacggcgtc
    29521 tgggttgtct ggtcaccggt gccatccagc cccaccacca gaccatagcc aatcagtgag
    29581 ttttgcctta ccccctgaac actggtgaga tcgcgaatac gctcagcctg agccgccgtc
    29641 gtaaccagta gaagaattaa tgcagagaga aatttaatca ccacagcctc acttacattg
    29701 gcgacaggtt aaggaagaaa cgctgcaacc agcccatatt ttgcgcttcg ttaatgtagc
    29761 cattgcctac gtattcaatg cgcgcatccg ccacctgagt agacggtacg gtattgctgc
    29821 cgctgatagt gcgtggatta accacgccag agaagcgaat aaattcggta ccctgattaa
    29881 tggcaatctg tttttcaccc accacatgca ggttgccgtt gaccagtacc tggtcaaccg
    29941 tcaccgtcaa cgtgccgcta aaggtattgc tggcattggc cccgcccttt ccgttgaacg
    30001 tgttaccacc ggaggcttcg acatcggcac gagcgttacc aaacagcccc tgcaaatagc
    30061 gcggcacagt atcaaagcca aaattagttt taccgtcacg gctggcattc gcagaggagc
    30121 ttttgctggc gctgacgttc tcctgcaaca cgatggtcag cgtatcgcca atattgcgtg
    30181 gtcgacgatc ttcaaacagc ggttgatagc catagttaat cggctgagca gactggaaaa
    30241 tagaaccgtt ggcgacgggc gtcggcccgg gaaccggttg tgcactggtc gccccctgca
    30301 ccagcggcgt ggagggtatc caggcgcagc cggttagtga aagcaccaac aagctggaaa
    30361 tggcataagt atgcgcagcg tttttttgca tggctatcat cttcaaaaat cagtaaaccg
    30421 gtgaacctgc caccggttaa gccttagagt tgcgtcagtt tttgcagcat ctgatcggtg
    30481 gtggacaccg ctttactgtt gatttcgtaa gcgcgttgca cctgaatcat attgaccagt
    30541 tcttccgcca cgttgacgtt agacgtttca acataccctt gatacagcag tcccgcgccg
    30601 ttcaggcccg gcgtgctttc gttcggtgca ccagaggatt gcgtttcggt gtagaggttt
    30661 tcgccaatgc tctccagccc ggtgtcattc ataaaggtgg tgagattgag ctgcccaacc
    30721 tgaaccggag ctgcctggcc ttgttgggtt acgctgacca cgccatcacg accgatggtg
    30781 atacttaacg cattcgccgg aatggtgatc gctggctgca cctgaaaacc accagccgtc
    30841 accagctgcc cgttctgatc cacctggaaa gagccgtcac gggtataggc tgatgaacca
    30901 tctggcaaca tcacctggaa aaagccctgc cctttaatcg cgacatcttt gctgttgttg
    30961 gtctgcgaca ggtttccctg gctgtgtaag cgttcagtgg cgaccgggcg tacccccgtg
    31021 ccgatttgta atccggaggg taaggtggtt tgttcggaag actgtgcccc cggctggcga
    31081 atggtttgat aaagcagatc ttcaaacacc gcgcgctgac gcttaaaacc gttagtactg
    31141 acgtttgcca ggttgttggc aatgacgtcc atattggttt gctgggcgtc aaggcccgtt
    31201 ttggcgatcc ataatgaact gatcataaaa tgtcctgtag tggattagtg acaagcagag
    31261 cagaagcacg cgacagtgtg aatttcccct caccctaacc ctctccccaa aggggcgagg
    31321 gaatagtccg cgcgctttta ttgcaatgtc atcaatcgtt caattaactc atcgacagca
    31381 gttggttggc acggcctgcg ttatcatcga cgctgctgat caccttcatc tgcatttcaa
    31441 aacgccgcgc gctggcaatc atgtcgctca ttgccgcaac ggcattgacg ttactgcctt
    31501 ccagaacccc cgacatcaca cgcaaggttg gatctgcctg cagtatcggc ccacgcgtgg
    31561 cctggctttc tgcgcttaaa cgaaaaatgc cgtcgtcacc gcgttgcact tcgctgcccg
    31621 tggctttcac cagtttcaga cgccccactg gcgcaaccgt atttgccgga tcgcccggat
    31681 tgagcgccga gattgtgcca tcggcagcaa tagtgatttc cgccccttcc ggcacagcaa
    31741 ttggcccagc ctcgcctatc accggatgcc cctgaattgt cagttgcccg gtgggatcaa
    31801 cctgaatgct gccattacgc gtataccctt cgctgccgtc agcggactgc acggccagcc
    31861 agccatcctg ctgcaacgca acgtccagcg ggcgcgaggt gtaatccatc ttgccgggcg
    31921 tcatgtctgc acccggtgtt gacgccgtta ccagcgtgcg cgtgggcagt gaaagccctt
    31981 ccaccggcac tgcacgcaag gcgtttaact gcgcgcgaaa accgggcgta gaggcattgg
    32041 ccagattact ggcggttacc gcctgttgat tcagcgtctg gctggctgct cccatcgcgg
    32101 tataaattgc gtgatccatt gagctatccc gtcagcgatt agcgtaagtt aaccagcgtg
    32161 ttgaggatct ggtcctgggt tttgatggtc tgggcgttag actgatagtt acgctgggca
    32221 acgatcatat tgaccagttc tttactgaga tcgacgttgg acgcttccaa cgcaccgttg
    32281 gtcagggtgc caaagttgcc cgtcccggct gtccccaaca gcgccacgcc agaagattgc
    32341 gtcgcagacc agacgttgtc gccttcggat gccagacctt cgttgttggc aaagttcgcc
    32401 agtacaatct gccccagcag ttgtgtttgt tcgttggaat agttgccgac aaccgtaccg
    32461 tcatcattga tttgataact caccagatcg cccggtttgt agccattctg ggtggttgcc
    32521 acaatattgt tagcgccggt attttgctgc atggagttga ggaagctcag gctaaacgtg
    32581 gcgggatctg cgccgttaat tgcgccagtg gtaatattct ctgttggatt tgaggtcaga
    32641 acgccattgg cattaaacac cagcttcatt gcaggctcgg ctgtacctgt tggatcactg
    32701 ctatcctggg tgtagacctg ccagttatta tccccggtct tcacaaagta gacgctcatg
    32761 tcatgagcat taccctgact gtcgaaaaca gtcaccgaac cttttttgtt atagctatcc
    32821 gcattgctgg cgctgaatgg cgtaacagta ggaagcggat cactggaatt caggttgatc
    32881 tgcatcgatg ccgtggtggt agttttcgct gccatcaggg tattcgggat cgaaatattg
    32941 gtcggattcg ccccttgctg aatagtcggc ggcgtaccgg ttgccgggta acccgtcagc
    33001 tgtaaacctt gcatattcac caggttacgg ttttcatcca gcttaaattg tccgttacgg
    33061 ctgtagaaca ccgaaccgtt gctgtctacc agacggaaaa aaccgttctg gctgatagca
    33121 acgtccagac ctcgcccggt gttggtggtc gtgccatcgg taaagtcctg agtgataccg
    33181 gcaactttta cccccagtcc cactttcgaa ccggcaaaca tatcggcaaa agaggccgtg
    33241 cctgatttaa agccgtaggt ggcggagttg gcgatattgt tgccaataac atcgaggttg
    33301 gtggcggcag cgtttaatcc gctaaccgct tgagaaaagg ccatgactga ctcctgaaat
    33361 gtgaaggctt aaattatctg ccgtacttcg tcgagggtgg tggtgccgta agtgccgaga
    33421 tccagcgtat taccgcggtt gccgcggatc acaccctgca ccagagcaaa ctgcagcggc
    33481 tgggcaacca gttgtgtacc accgttactc gcgctaatcg ctacattgta agaaccgttc
    33541 ggcgcagtgc tgccgtcagt caacgtaccg tcccaggtga aactgtgaac tccggcggtc
    33601 agttcaccaa tatcaatggt gcgcacaacc gcaccatttt tatcggtgat ggtggccgta
    33661 actttgtctg ccgcctgttg cagctcaaca ccaaacggcg tggtcgtggt cacagctcct
    33721 tcttcactgc cggttcccgc aagaacagtg gtgccgggga tcatcacgcc gtgaccgatc
    33781 aggttactgg cctgtaacga ctggctgtta tcaatctgtc cggaaataga tccgagtgtg
    33841 gtattgagtt tttcaatccc gctgaccgtg ctgatttgtg ccaattgcga cgtcagctcg
    33901 ttgttttcca ttggattggt cgggtcctgg tttttcagct gcgccaccag caaagtcaga
    33961 aaactgcttt gtaaatctgc ggcgttgctg cccgtgagcg aactactgct ggtggtactg
    34021 acgccggtat ttgtcggatc ggtggtggtt accgcaatgg acatagcttt ctcctttatt
    34081 gaccgagcgt aagggttttc agcatcatac ttttcaccgt gttgagcact tcaacattgg
    34141 cctgatagct gcgtgacgcc gacatggtgt taaccatctc cccgacgaca tcaacgttcg
    34201 gcatttttac gtagcccttt gcatccgcca gcggattacc cggttcataa accagtttgt
    34261 ccggggcctg actttctata acatcggcaa cctttacgcc gcctgtcgca gcacctggtg
    34321 cagcgttaac ctggaatacc acctgttttg cccgatatgg ctgtccatcg ggaccggtca
    34381 cgctatcagc atttgccaga ttactggccg ccacgttcag gcgctgggac tgggcagtta
    34441 acgccgaccc ggcgatatca aaaatattca gcagtgccat ccgttaattt ccgctctgta
    34501 aaacgttcat catgcctttg atttgcccgc tcaacgcgct aaggctcatc tggtattgca
    34561 ggctgttatc ggcaaactgg gtgcgttcgc gatccatatc gacggtatta ccgtcaagcg
    34621 aaggctggtc cggaatacgg tattgcagtt ctgcggtagg aggcgtcagc gcctgcgccg
    34681 ggatgtgttg cgttgaggtc atcgtcagtg caaccacact ggttgcatcc cgtccacgtt
    34741 gcatgacttt tttaagttca ctggcaaaat cgatatcgcg cgcctgataa ccaggggtat
    34801 cggcattggc gatgtttgct gccagcactt cctgacgctg ggcgcgcaga ttgagcgcct
    34861 cttgttgaaa acgtaaggcg gcgtcgagct tatcgagcat atctcctccg caggtatcaa
    34921 aattctgcca tcagcttaaa tgcctttacg cgcgcgttat cggcggaata aacgcaaaat
    34981 gggtcgctat ttatgccgtt gatggtcatt gcggacaggt acaattcacg ttgtagaaat
    35041 ggctgggggc gaaaatgctg acaataaaac gtagcgtggc gatcatcgcg atactgttca
    35101 gtccgttaag tacggcgagc aatctcacat cgcaattgca caactttttt agcgcccaac
    35161 tcgcgggggt aagtgatgag gttcgtgttt ctattcgtac agcgcccaat ctactaccgc
    35221 catgcgagca gccattgctt tcgatgagca ataattcccg cctgtggggc aatgtgaatg
    35281 tgttggcacg ctgcggtaac gacaaacgat atttacaggt taatgtgcag gccacaggaa
    35341 attatgtggt tgccgcgatg cccattgtgc ggggaggaaa gctggaagct ggcaatgtca
    35401 aactaaaacg cggacggctg gataccctgc caccgcgtac ggtgctggat atcaatcaac
    35461 ttgttgatgc cattagcctg cgcgatctat cacccgatca acctatccag ttaacccagt
    35521 ttcgccaggc atggcgggta aaagcgggac aacgcgtcaa tgtgatcgcc agcggtgatg
    35581 ggtttagcgc caacgcagaa ggtcaggcgc tgaacaatgc agccgtcgca cagaatgcgc
    35641 gggtgcgcat ggtatcggga caggtagtca gcggcgttgt tgatgcagat gggaatattc
    35701 ttataaacct gtaagctgtt aaagattacc cgtcccttgc cgataaataa gcaacacatg
    35761 ataaaagcgc cctcaatgag gaataaacca tgagtattga tcgcacttcg cctctgaagc
    35821 ctgtaagcac cgttcaaccg cgcgaaacca ctgacgcgcc ggtaacgaac agccgggcgg
    35881 caaaaacaac cgcctccacc agcaccagtg tgacgttaag cgacgcgcaa gcaaaactga
    35941 tgcaacccgg cagcagtgat atcaatcttg aacgtgtcga agcgttaaaa ctggcgattc
    36001 gtaacggtga actaaaaatg gacaccggca aaattgccga tgcgctgatc aacgaagcgc
    36061 agcaagactt gcagagtaac tgatcgtatg acacgtcttg cagagatcct cgaccagatg
    36121 tccgctgtgc ttaacgatct caaaacggta atggatcaag agcagcaaca tctctctatg
    36181 gggcagatca acggcagcca gttgcaatgg attacagaac aaaaaagctc actgctggcg
    36241 acgctggatt acctcgaaca gttacgcaag aaagaaccca atacagcaaa tagcgttgat
    36301 attagtcaac gctggcagga aattactgtg aaaacgcagc aactacgcca aatgaatcaa
    36361 cataacggct ggttactgga aggacagatt gagcgcaatc aacaggcgct ggaaatgttg
    36421 aaaccgcatc aggaaccgac gctatatggg gcgaacggtc agacctcaac aacccatcgc
    36481 ggcggtaaaa agatttcgat ctgaatatta tctccggcct gcactgcagg ccggaatgca
    36541 ttgttacacc gtccggcggg caaattcttt aactttgaag cccagtaccg ccagtgcagc
    36601 gaagtacgcg gcaatccccg ccagcacgac cgccattaaa cgcagtaagc gccagggcat
    36661 ggtacccaat gaccactccg gcatgatatg taacatacct aaaagcacgc cagacatcac
    36721 cagtaccgcc accaccagac gcaacagaaa cgccatccag ccgggttgcg gagtaaagat
    36781 tttctgctta cgcaactgcc agtaaagcag cgaagcattc agacacgccg ccagaccaat
    36841 agaaagtgac agcccggcat gtttcaacgg accaataaac gccaggttca tcaattgcgt
    36901 taaaatcagc gtaacgatgg caattttcac tggcgtttta atgtcctggc gggaataaaa
    36961 gccaggagcc aacactttca ctacaatcag gccaatcaaa cccaccgagt aggcaattaa
    37021 cgcccgctgg gtcatcagcg catcaaacgc ggtaaattta ccgtactgga acagcgaaac
    37081 ggttaacgga cctgaaagaa tgcccaacgc aaccgcactc ggcagcgcca acaggaaaca
    37141 aagacgcaat ccccagtcca tcaaacggtt gtattcatca tgattgccac tggcaaaact
    37201 tttcgacagc gacggcagca aaatggtgcc aagcgccacg cccagcacac cggacggaaa
    37261 ctccattaag cggtcggcgt aatacatcca agacaccgaa ccggaagcaa gaaacgaggc
    37321 aaaaatggtg ttgatgatta aggagatctg gctcacagag acgccaagga tcgccggtcc
    37381 catctgtttc accacgcgca ttgctccggc atcgtggaag ttaatgcgcg gcaggaccag
    37441 catgccgatc ttcttcaggt gcggtagctg atacaccagc tgcaggatgc cgccgaccgt
    37501 aacagcccac gccagcgcca gcaccggtgg gttaaagtac ggtgcggcaa acagcgcgaa
    37561 accaatcatg ctgatgttaa gcagtgttgg agcaaacgcc ggaatcgaga agcggttcca
    37621 cgtattcaga atcgctccca ccagcgacgc cagggagatc agcaagatat agggaaaggt
    37681 aatctttagt agctggctgg tcagggcaaa tttgtcagct gtgtcagcga agcctggcgc
    37741 ggtcaccatg atcacccacg gtgcggcgag catgccagcg accgtcacaa ccgccagcgc
    37801 gagtgtcagc aggccagaaa cataagagac aaagacccgc gtggcgtctt caccctgctt
    37861 acttttatat tccgccagaa tcggtacaaa tgcctgggaa aatgcccctt cggcaaagat
    37921 acggcgtaac aagttaggaa gtttaaaagc gacgaaaaag gcgtcggttg ccatccctgc
    37981 gccaaagatt ctggcgacaa ttgcgtctcg tgcgaagcca agcacacgcg aaaacatggt
    38041 catcgagctg acggcggcca gcgattttaa taaattcatc ggtgttctaa tccagaccca
    38101 cgggcaaaac gcctgcaagg caggcgctga aagaggcgaa tagtctacct ggattatggt
    38161 gaattgctac cgccagatgt tacagggttt attcactcat cgcatcgcgc cagatcttgt
    38221 caacgatacg ttgcgccagc acggcctgtt cgccggcggt ttgcggaact gtctgatttt
    38281 gcacacattc aatgaagtgc cgcgcacagc cgacaaatcc acgttgttca agagtgctct
    38341 gccaaccagg aatcggttta tgcactacgc cctgcccgcg ctcctcacgc cattcgcgca
    38401 tatccgtaat gtcgatgagc gcaccgtcag tcacggcctg cacggtttca cgctgacttc
    38461 cggcacggcg atgcatacag gtggtgatct gcaaaggacc agccgaaaaa tggtgctcgg
    38521 caaacagcat ttcgccagcg tcgttagtca gtagcgtacc gccatccaga gaggctttgc
    38581 cacccgacaa ccacagcgcg gtatccacca catgcagata atcatccagc aacgtgaaat
    38641 aaagatcgtg tggcccgacg ctattgctac gatgtttatc cattcttaac gaggctgcgg
    38701 tggcgagttg cgtttttaac tctccgtaga gtggtgcgaa acgacggtta aaaccgacca
    38761 tcaacgtcag ttttttccgc gccgccagtt ccaccagccg ttcagcatcg cgcagatttt
    38821 ctgccagcgg tttatcgaca cagacatgta cccccgcatt gagtaacgta ctgaccacgt
    38881 caaagtggct ggcggtgctg gaatgcacaa aaaccgcatc gcaactggcg gcaaggctgg
    38941 ataacgaatc ggcataagga atgcgccagc tttcacaaat tggcagggct ttcgcgcgcg
    39001 taggcgacca ggctccttgt aacatccagt cagacgctgc cgccagcacc ggtaaccacg
    39061 ctttttgcgc aatgccgcct aatcccacaa cgccgatacg taattttttc actttaatct
    39121 cccagatggg ccagcaacga atcaagacgc tgtttcagtt ctgccacttc gatttccagg
    39181 gcttcgacgc gggcctgtaa atcaccatca accgcgttcg acatatccgt taccgccggc
    39241 tgatcctcta cctcaccact gaaaagatgc atgtagcggc tttcgcgttt acccggttcg
    39301 cgggccagac gcaccacaaa aggaccatct tcgcgatttg ccagttgttc cagcgtcgac
    39361 tccacttccg ccatatcgct gaattcatac attcgcgcgg cgcggctgcg cagttcacct
    39421 ggcgtctggg caccacgcaa taacaacgtg gtgattaacg ccacttccgc tgcgctcagt
    39481 ttcagatcgc caaattctga attacaaaaa cgttgctcat atttggtgac ccgattacca
    39541 aaaccgctca ctgtgcgtag ataatgacgt ttgaccagat tatccagctg ttcctgcact
    39601 tcggattcac tcagattcat gaccggttca cggttcgttt tctgattaca ggccgtgact
    39661 acaccattga ctgagagcgg gtattgctcc ggcgtggtca cctgcttttc cagcaagcaa
    39721 ccaatcacgc gagcttcaag ggcagtaagt tgatatttca tgagattctc cttagcggcc
    39781 gggcgtccag tctggggtag ttaatgccgt cagtacgtga tcgcgccatt gtccatcaat
    39841 caacagatag tctttcgcat agccttcttt ttcaaaaccc agtcgcgcca gtaaatcacc
    39901 gctgcgttta ttgtgcggca tataattagc cataatgcga tgaatatgtt gggtgcgctg
    39961 catataacga atggctgcgg tcagggcttc aaacatgagt cctttgccct gccatttttg
    40021 cccaatcgaa taaccgagat agcaggcatg aaaagagcca cgaacaacat tggaaaaatt
    40081 ggcaacgcca ataatctctt tttcgtccgg gtcgaataag ccaaagtaga aagctgaacc
    40141 ttgtttatga aattcgttaa tcatccccag cctggcctgc cagcctgatg gataacagtg
    40201 gctttcgtcg cgcactggct cccagggctt gaggaaatgg cgattctctg cgtaataatc
    40261 cgcaagacgc caggcatcac gatcatgcac cagacgcacg accagtcggt ctgtggttaa
    40321 gcgcactttt ggcacgttac tgcgatagcc aaacattcta tacctactcc ttcccgtaac
    40381 gtctttaata cgctgtaagt attactatac ctgcgcaacc agaccctgtg aaaagtccaa
    40441 aatgccattt tctgcattat tcacatattt gataaaaact ctctatcaaa gcgacgtttt
    40501 gataaaaaaa tattgtcccc gaagtgatgg gaaaagtctt agcggcaatg cagaatagaa
    40561 agtgctcatc ttttatttcc cgggagggga aatgtcccgc gtatcgcagg cgaggaacct
    40621 gggtaaatat ttcctgctca tcgataatat gctggtcgtg ctggggttct ttgttgtctt
    40681 cccgctgatc tctatccgct tcgttgatca aatgggctgg gccgccgtca tggtcggtat
    40741 tgctctcggt ctacgccagt ttattcagca aggtctgggt attttcggcg gcgcaattgc
    40801 cgaccgcttt ggtgccaaac cgatgattgt taccggtatg ctgatgcgcg ccgccggatt
    40861 cgccacaatg ggtatcgccc acgaaccgtg gctattgtgg ttttcatgcc tgctctcggg
    40921 actcggcggc acgttgtttg atccgccgcg ttcggcgctg gtggtgaaac taatccgtcc
    40981 acaacaacgt ggtcgttttt tctcgctgtt gatgatgcag gacagtgccg gtgcggtcat
    41041 tggcgcattg ttggggagct ggctgttgca atacgacttt cgcctggtct gcgccacagg
    41101 ggccgttttg tttgtgctgt gtgcggcgtt caatgcgtgg ttgttaccgg catggaaact
    41161 ctccaccgta cacacgcccg ttcgcgaagg catgacccgc gtgatgcgtg acaagcgttt
    41221 tgtcacctat gtcctgacgc tggcgggtta ctacatgctg gctgtacaag tgatgctgat
    41281 gctgccaatt atggtcaacg acgtggctgg cgcaccctct gccgttaaat ggatgtatgc
    41341 cattgaagcg tgtctgtcgt taacgttgct ctaccctatc gcccgctgga gtgaaaagca
    41401 ttttcgtctg gaacaccggt tgatggctgg gctgttgata atgtcattaa gcatgatgcc
    41461 ggtgggcatg gtcagcggcc tgcaacaact tttcaccctg atttgtctgt tttatatcgg
    41521 gtcgatcatt gccgagcctg cgcgtgaaac cttaagtgct tcgctggcgg acgcaagagc
    41581 tcgcggcagc tatatggggt ttagccgtct gggtctggcg attggcggcg ctattggtta
    41641 tatcggtggt ggctggctgt ttgacctggg caaatcggcg caccagccag agcttccgtg
    41701 gatgatgctg ggaattattg gcatcttcac tttccttgcg ctgggttggc agtttagtca
    41761 gaaacgcgcc gcgcgtcgtt tgctggaacg cgacgcctga tttgatactc ccccctgact
    41821 cctccatact gaaagtgaac tgacagccaa atggaggagt cacgtgaagc tatacattta
    41881 cgatcactgc ccttactgcc tcaaagcccg catgattttc ggcctgaaaa atatccccgt
    41941 cgaattacat gttctgctca acgacgacgc agaaacaccc acccggatgg tcggtcaaaa
    42001 acaggttccc attctgcaaa aagatgacag ccgctatatg ccagaaagca tggatatcgt
    42061 tcactatgtc gataaactcg acggcaaacc gttactgacc ggcaaacgtt cccctgccat
    42121 tgaagagtgg ctgcgcaagg tcaatggcta cgcaaacaaa ctgctgttgc cgcgttttgc
    42181 caaatcggca tttgatgagt tttctactcc cgccgcgcgc aaatatttcg tcgacaagaa
    42241 agaggccagc gcgggtaatt ttgccgacct gctggcccac tctgacggtc tgattaagaa
    42301 tatcagcgat gatttgcgtg cgctggacaa actgatcgtc aaaccgaacg ccgtgaatgg
    42361 cgaactttcg gaagatgata ttcagctatt tccgctactg cgtaatctga cgctggtagc
    42421 cggaatcaac tggccaagcc gcgttgctga ttaccgcgat aatatggcga aacagacaca
    42481 aatcaatttg ttatcatcaa tggcgattta atcctgaccc gccgcggcag cctgtattcg
    42541 cgggttgccg ccgttttcag ttttttctgc tggcgagttt atcgctggtg acacatgctg
    42601 cgttgtgtca ggatattgac gcagatttca tgtcgaggac taccatgaac aagtttttat
    42661 ttgccgctgc attgatcgtc agtggcctgc tcgttggctg taatcaactc acccaataca
    42721 ccatcaccga acaagaaatt aaccagtcgc ttgcgaaaca taataatttc tcaaaagata
    42781 tcggtttacc cggcgtggct gacgcccata ttgttctgac aaacttgacc agccaaattg
    42841 gtcgcgaaga gccgaataag gttaccctaa ccggagacgc caatctgaca tgaactccct
    42901 gttcggtagt cagaaagcga ccatgaaact gaagctgaaa gcgctgcccg tgtttgataa
    42961 agaaaaaggt gcgatcttcc tgaaagagat ggaggtggtc gatgcgacgg tacaaccgga
    43021 aaaaatgcaa acggtgatgc aaacgttgct tccctatttg aaccaggcat tacgcaatta
    43081 ctttaaccag caacctgctt acgtcctgcg cgaagatggc agtcagggcg aagcaatggc
    43141 gaaaaaactg gcgaaaggca ttgaagtgaa gccaggcgaa attgtcattc cgtttacgga
    43201 ttaatgacga gggcgcattc gcgcccttta tttttcgtgc aaaggaaaac gtttccgctt
    43261 atcctttgtg tccggcaaaa acatcccttc agccggagca tagagattaa tgactgcacc
    43321 atcccaggta ttaaagatcc gccgcccaga cgactggcac cttcacctcc gcgatggcga
    43381 catgttaaaa actgtcgtgc cgtataccag cgaaatttat ggacgggcta tcgtaatgcc
    43441 caatctggct ccgcccgtga ccacagttga ggctgccgtg gcgtatcgcc aacgtattct
    43501 tgatgccgta cctgccgggc acaatttcac cccattgatg acctgttatt taacagattc
    43561 gctggatcct aatgagctgg agcgcggatt taacgaaggc gtgttcaccg ctgcaaaact
    43621 ttatccggca aacgcaacca ctaactccag ccacggcgtg acgtcaattg acgcaatcat
    43681 gccggtactt gagcgcatgg aaaaaatcgg tatgccgcta ctggtgcatg gtgaagtgac
    43741 acatgcagat atcgacattt ttgatcgtga agcgcgcttt atagaaagcg tgatggaacc
    43801 tctgcgccag cgcctgactg cgctgaaagt cgtttttgag cacatcacca ccaaagatgc
    43861 tgccgactat gtccgtgacg gaaatgaacg gctggctgcc accatcactc cgcagcatct
    43921 gatgtttaac cgcaaccata tgctggttgg tggcgtgcgt ccgcacctgt attgtttacc
    43981 catcctcaaa cgcaatattc accaacaggc attgcgtgaa ctggtcgcca gcggttttaa
    44041 tcgagtattc ctcggtacgg attctgcgcc acatgcacgt catcgcaaag agagcagttg
    44101 cggctgcgcg ggctgcttca acgccccaac cgcgctgggc agttacgcta ccgtctttga
    44161 agagatgaat gctctgcagc actttgaagc attctgttct gtaaacggcc cgcagttcta
    44221 tggcttgccg gtcaacgaca cattcatcga actggtacgt gaagagcaac aggttgctga
    44281 aagcatcgca ctgactgatg acaccctggt gccattcctc gccggggaaa cggtacgctg
    44341 gtccgttaaa caataaaaat cgtagcttcc tgttgtcatt aggttatttt acctgtataa
    44401 ataaccagta tattcaacag ggggctatta tgcgaattga agtcaccata gcgaaaactt
    44461 ctccattgcc agctggggct attgacgccc tggctggcga actttcccgc cgtattcagt
    44521 atgcgtttcc tgataatgaa ggccacgtat cggtacgtta tgccgcagcg aataatttat
    44581 cggttattgg cgcgacaaaa gaagataaac agcgcattag cgaaattctc caggaaacgt
    44641 gggaaagcgc cgatgactgg tttgtcagcg aataatatgc agtgattttt gtttttgccg
    44701 ggtcgccccg gcttttttat tttacttacg aattttgagt acaaaatttc tacacttcct
    44761 caaaataatt gcacaatatg
//
LOCUS       DAZGVQ010000021        42477 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000021 DAZGVQ010000000
VERSION     DAZGVQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.021"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(18..2447)
                     /gene="hrpB"
                     /locus_tag="ACHLAG_001132"
     CDS             complement(18..2447)
                     /gene="hrpB"
                     /locus_tag="ACHLAG_001132"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFP0654401.1"
                     /translation="MSSLPVAAVLSELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNLPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            2521..3051
                     /gene="thpR"
                     /locus_tag="ACHLAG_001133"
     CDS             2521..3051
                     /gene="thpR"
                     /locus_tag="ACHLAG_001133"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFP0654402.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            3066..3770
                     /gene="sfsA"
                     /locus_tag="ACHLAG_001134"
     CDS             3066..3770
                     /gene="sfsA"
                     /locus_tag="ACHLAG_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFP0654403.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAELSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            3948..4403
                     /gene="dksA"
                     /locus_tag="ACHLAG_001135"
     CDS             3948..4403
                     /gene="dksA"
                     /locus_tag="ACHLAG_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFP0654404.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            4470..5366
                     /gene="gluQRS"
                     /locus_tag="ACHLAG_001136"
     CDS             4470..5366
                     /gene="gluQRS"
                     /locus_tag="ACHLAG_001136"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFP0654405.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            5426..6823
                     /gene="pcnB"
                     /locus_tag="ACHLAG_001137"
     CDS             5426..6823
                     /gene="pcnB"
                     /locus_tag="ACHLAG_001137"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFP0654406.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYDTYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            6820..7299
                     /gene="folK"
                     /locus_tag="ACHLAG_001138"
     CDS             6820..7299
                     /gene="folK"
                     /locus_tag="ACHLAG_001138"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFP0654407.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            7669..8253
                     /gene="yadN"
                     /locus_tag="ACHLAG_001139"
     CDS             7669..8253
                     /gene="yadN"
                     /locus_tag="ACHLAG_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0654408.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            8358..9098
                     /gene="yadV"
                     /locus_tag="ACHLAG_001140"
     CDS             8358..9098
                     /gene="yadV"
                     /locus_tag="ACHLAG_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFP0654409.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            9133..11733
                     /gene="htrE"
                     /locus_tag="ACHLAG_001141"
     CDS             9133..11733
                     /gene="htrE"
                     /locus_tag="ACHLAG_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFP0654410.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGSLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEKGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            11750..12319
                     /gene="yadM"
                     /locus_tag="ACHLAG_001142"
     CDS             11750..12319
                     /gene="yadM"
                     /locus_tag="ACHLAG_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0654411.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            12334..12936
                     /gene="yadL"
                     /locus_tag="ACHLAG_001143"
     CDS             12334..12936
                     /gene="yadL"
                     /locus_tag="ACHLAG_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFP0654412.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            12963..13559
                     /locus_tag="ACHLAG_001144"
     CDS             12963..13559
                     /locus_tag="ACHLAG_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFP0654413.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            13543..14886
                     /locus_tag="ACHLAG_001145"
     CDS             13543..14886
                     /locus_tag="ACHLAG_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFP0654414.1"
                     /translation="MCTTNNRSKQITATVTAVDGGVIMKKFFRHFLFLILCLSCYTAS
                     AGTDDNVGYIVGNSYGVGPSDQKWRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTG
                     VKLQWSQLDTASGGGFLYCNRSDSTSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYY
                     TLLISNMWSAYGTVTNVSSPGIYIGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAV
                     GGIYQTLTIEFYTDTNFDPTVTQQIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFD
                     LLNVKLTNPTCFTAMLSGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNI
                     ETKLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKD
                     YDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            14999..15793
                     /gene="panB"
                     /locus_tag="ACHLAG_001146"
     CDS             14999..15793
                     /gene="panB"
                     /locus_tag="ACHLAG_001146"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFP0654415.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVEDIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            15805..16656
                     /gene="panC"
                     /locus_tag="ACHLAG_001147"
     CDS             15805..16656
                     /gene="panC"
                     /locus_tag="ACHLAG_001147"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFP0654416.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(16730..17631)
                     /gene="rpnC"
                     /locus_tag="ACHLAG_001148"
                     /pseudo
     CDS             complement(16730..17631)
                     /gene="rpnC"
                     /locus_tag="ACHLAG_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
     gene            17905..18285
                     /gene="panD"
                     /locus_tag="ACHLAG_001149"
     CDS             17905..18285
                     /gene="panD"
                     /locus_tag="ACHLAG_001149"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFP0654417.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(18289..19518)
                     /gene="yadE"
                     /locus_tag="ACHLAG_001150"
     CDS             complement(18289..19518)
                     /gene="yadE"
                     /locus_tag="ACHLAG_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP0654418.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(19582..20022)
                     /gene="yadI"
                     /locus_tag="ACHLAG_001151"
     CDS             complement(19582..20022)
                     /gene="yadI"
                     /locus_tag="ACHLAG_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP0654419.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(20127..20897)
                     /gene="yadH"
                     /locus_tag="ACHLAG_001152"
     CDS             complement(20127..20897)
                     /gene="yadH"
                     /locus_tag="ACHLAG_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0654420.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(20894..21820)
                     /gene="yadG"
                     /locus_tag="ACHLAG_001153"
     CDS             complement(20894..21820)
                     /gene="yadG"
                     /locus_tag="ACHLAG_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0654421.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            21929..22591
                     /gene="can"
                     /locus_tag="ACHLAG_001154"
     CDS             21929..22591
                     /gene="can"
                     /locus_tag="ACHLAG_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFP0654422.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(22632..23168)
                     /gene="hpt"
                     /locus_tag="ACHLAG_001155"
     CDS             complement(22632..23168)
                     /gene="hpt"
                     /locus_tag="ACHLAG_001155"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFP0654423.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            23374..25764
                     /gene="gcd"
                     /locus_tag="ACHLAG_001156"
     CDS             23374..25764
                     /gene="gcd"
                     /locus_tag="ACHLAG_001156"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFP0654424.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQL
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(25811..27361)
                     /gene="cueO"
                     /locus_tag="ACHLAG_001157"
     CDS             complement(25811..27361)
                     /gene="cueO"
                     /locus_tag="ACHLAG_001157"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFP0654425.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIILPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            27527..27874
                     /gene="yacC"
                     /locus_tag="ACHLAG_001158"
     CDS             27527..27874
                     /gene="yacC"
                     /locus_tag="ACHLAG_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFP0654426.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            27980..28846
                     /gene="speE"
                     /locus_tag="ACHLAG_001159"
     CDS             27980..28846
                     /gene="speE"
                     /locus_tag="ACHLAG_001159"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFP0654427.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            28862..29656
                     /gene="speD"
                     /locus_tag="ACHLAG_001160"
     CDS             28862..29656
                     /gene="speD"
                     /locus_tag="ACHLAG_001160"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFP0654428.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(29694..30056)
                     /gene="yacL"
                     /locus_tag="ACHLAG_001161"
     CDS             complement(29694..30056)
                     /gene="yacL"
                     /locus_tag="ACHLAG_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFP0654429.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(30232..32829)
                     /gene="acnB"
                     /locus_tag="ACHLAG_001162"
     CDS             complement(30232..32829)
                     /gene="acnB"
                     /locus_tag="ACHLAG_001162"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFP0654430.1"
                     /translation="MLEEYRKHVAERAAEGIAPNPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            33184..34941
                     /gene="yacH"
                     /locus_tag="ACHLAG_001163"
     CDS             33184..34941
                     /gene="yacH"
                     /locus_tag="ACHLAG_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFP0654431.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVTQTVTAPVIPSN
                     TVSTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(35012..36436)
                     /gene="lpdA"
                     /locus_tag="ACHLAG_001164"
     CDS             complement(35012..36436)
                     /gene="lpdA"
                     /locus_tag="ACHLAG_001164"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP0654432.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(36761..38653)
                     /gene="aceF"
                     /locus_tag="ACHLAG_001165"
     CDS             complement(36761..38653)
                     /gene="aceF"
                     /locus_tag="ACHLAG_001165"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFP0654433.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(38668..41331)
                     /gene="aceE"
                     /locus_tag="ACHLAG_001166"
     CDS             complement(38668..41331)
                     /gene="aceE"
                     /locus_tag="ACHLAG_001166"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFP0654434.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(41492..42256)
                     /gene="pdhR"
                     /locus_tag="ACHLAG_001167"
     CDS             complement(41492..42256)
                     /gene="pdhR"
                     /locus_tag="ACHLAG_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFP0654435.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT        10563 a        11134 c        10333 g        10447 t
ORIGIN      
        1 gagggagaaa accaacctta cgaatacttt ttcgtccgtc gcgtcggtgc agtatttgcc
       61 gggtcgtccg gccagacatg tttgggataa cgccctttca tctctttttg cacctcacgg
      121 tacgctcctt tccagaaggc gctcaaatct cgtgtgattt gtaatggcct ttgggcaggt
      181 gaaagcaact ccagcaccag cggcacgcgc ccctgggcga tcgtcggatt ggtggcctcg
      241 ccaaacatct cttgcattct caccgccagc gcgggcaggt tatcttcatg ataacgaatg
      301 gcgatccggc ttcccgtcgg cacagtgtaa tgcgcaggca attcactatc cagacgttgc
      361 tgcattcccc aatcaagtaa tccgcgtagt gcctgataaa tgtcgagtga tttcaggccg
      421 cgtagtgaat gtacgccagt catatgtggc agcagccacg tttccagcgc tgccaataaa
      481 ctttcatcat caaccgctgg ccagtcatat tccggcaacc actttgcggc gcataacaaa
      541 cgcaagcgta gctgttccgc ttccgccgtc cagttgagca cgcttaaacc tttatcacgg
      601 atgccattaa gcatcgcctg atgcaactcg tcttctgacg gtttcgccag cggctgcact
      661 ttcaccgtca actgaccgat ttgtagccga cgccaggctt tcagcgtacc ttgcgcgtca
      721 tcccactcca cagtgtcaga ctgctgtacc agctgcgggc agcgttgtac taactcatca
      781 atatcgaccg gcagcgccag taaaatccgc gcatccggcg aggcgctgcc ctgcaataat
      841 aacggtgcga tcaaccattc gtggcggctt agcgcgtcgt tggcatcgag catcgctccc
      901 atgccgtttg ccagttgata gcgtccatct tgcccacggc gacgagcaat gcgatcggca
      961 aacgccccgg caagtagcgg cgcgataagc gaactgtctg cctcaccgcc acgtacgttt
     1021 aagcgtttta acagttgctg actacgttgc tgccaggctg gttgattgcg cgaaaacgcc
     1081 acgcccaggt cactattgcc catccgtggc ggctcttcga gaatggcggc aatttttgcc
     1141 gcggtagcag cttcgtcgtc gttcttcgcg ctaaccagca ttgccgctaa acgcggatcg
     1201 ttacccagcg ctgccatttt ttgcccttgc gcactaagcc gttcaccctc cagtgccccc
     1261 agcatttgta acagacgttt cgcggccagt agattcacta ctggcggttg atccagccag
     1321 ctcatctgcg ccggatcgct gcatccccat tgcagtaatt ccatcagcaa accggaaaga
     1381 tcgctttgta agatctccgg ttcactttgc gccgcggcgc gttctgcttg ttctttggcg
     1441 attaaatgca ggcagatacc cggctccaga cgcccggcgc gcccggcacg ctgcgtcatg
     1501 gatgcctggc taacgcgttg agtaatcagt cgcgtaagcc ccgtgcgcgg atcaaaacgc
     1561 gccacacgct cctgggcaca atccaccacc agacgaatac cttcaatggt taaactggtt
     1621 tcagcaatat tggtcgccag caccactttg cgcatccctt gcggtgccgg gaggatcgct
     1681 tttcgctgat cgttcagcga caacgcgcca tacagcgggc agagcaatac atcactgccg
     1741 atgcgcgaag ccagttgttc ctgcacacgc tgaatttctc cgacgccagg taaaaataac
     1801 agtaatgatc cgctttcctg acgcagcatt tcagcggtgg ctaccgcaac ggcatcgtca
     1861 aaacgctgat gcgcgggcag cggtaaataa cggcgttcaa ccggaaacga gcgcccttct
     1921 gagatgacga caggcgcttc tggcagcatt tgctgcaagc ggtcgttgtc cagggtagcc
     1981 gacataatca gcagtttaag gtcatcacgc agaccttgtt gcacatcgag taacagcgcc
     2041 aacgccaaat ccgcctgcaa gctgcgctca tgaaattcat caaggatcac cagtccaaca
     2101 ccgctcagtt ccgggtcacg ctggatcatg cgcgtcagca cgccttcggt aaccacttcc
     2161 aggcgggtat tcggcccgac gcagttttgc gcacgcatcc ggtagccaac ggtatcgcct
     2221 ggcttttcgt taagcagctc cgccagccgt tgcgcgacgt tacgcgccgc cagacgacgc
     2281 ggctccagca ggataatttt cccgttaatg ccgggatgcg ccagcagttg cagcggcagc
     2341 caggttgatt tcccggcccc ggtcggcgca cttaataata cctgcggcgc acaatcgaga
     2401 gcggtaagta attcagataa gacggcagca acgggcaacg acgacacaaa acgctccaga
     2461 gggttaacat tcttcgcgcc acattgtagc atcgcggtaa ttcataaccg agtgcctcac
     2521 atgtctgaac cgcaacgtct gttctttgct atcgacttac ctgcagaaat ccgcgaacag
     2581 attatccatt ggcgcgccac acacttccca cctgaggcgg gacgtccggt cgccgccgat
     2641 aatttgcatc tgactctggc atttttaggc gaagtgagcg ccgagaaaga gaaggcgctt
     2701 tctcttttag ccggacggat tcgtcaacct ggtttcacac tcacgcttga tgacgccgga
     2761 caatggctgc gttcgcgtgt ggtgtggtta gggatgcgtc agcctccacg cggcttaatc
     2821 cagctggcga atatgctccg ttcacaggct gcccgcagcg gttgttttca aagcaatcgt
     2881 ccgtttcatc cacatattac cttattgcgc gacgccagcg aggcggtgac aatcccgccg
     2941 ccaggtttta actggtctta tgcggtgacg gagttcaccc tttacgcctc ctcgtttgcc
     3001 cgtggacgca cacgctacac gccgctaaaa cgctgggcgc taacgcaata acaaggattg
     3061 tcgcaatgga attctctccc cctctacagc gcgcgacgct aattcagcgt tacaaacgtt
     3121 ttttagccga tgtgatcaca cccgatggtc gcgaattaac gctacactgc ccgaatacgg
     3181 gtgcaatgac cggttgtgca acgcctggcg ataccgtctg gtattcgact tcagacaaca
     3241 ccaaacggaa atacccacac acctgggaat taactcaaag ccagagcggc gcatttattt
     3301 gcgtcaacac gctttgggct aacaggttga cgaaagaggc tatccttaat gaatcaattt
     3361 cagaactgtc aggctatagc tcgctgaaaa gcgaagtaaa atacggcgca gaactcagcc
     3421 gtattgactt tatgttgcag gcggattcgc gtccagactg ctatattgaa gtgaaatcgg
     3481 ttacgttagc ggagaacgaa cagggatatt ttcccgatgc ggtcactgaa cgaggtcaga
     3541 aacaccttcg ggagttgatg agcgtagcgg ctgaaggcca gcgtgcggtt atctttttcg
     3601 ccgtgctgca ttcagccatt acacggtttt cacccgcgcg ccacatcgat gagaaatacg
     3661 cgcaactatt gtcagaagct caacagaggg gggtagaaat tctggcttac aaagcggaaa
     3721 tttctgctga aggcatggct cttaaaaaat cactgccggt tacattgtag taaagtaagt
     3781 aactggttaa tttacattct ggtcgcgtgc gcaaatacgc ttttcctcac acagttgtca
     3841 agtgttacgt ttagataatt gctatccgga aaagcatctg ctatttatag cggcctcatt
     3901 tttcccccga acatggggat cgatagtgcg tgttaaggag aagcaacatg caagaagggc
     3961 aaaaccgtaa aacatcgtcc ctgagtattc tcgccatcgc tggggtggaa ccatatcagg
     4021 agaagccggg cgaagagtat atgaatgaag cccagctggc gcacttccgt cgtattctgg
     4081 aagcatggcg taatcaactc agggatgaag tcgatcgcac cgttacacat atgcaggatg
     4141 aagcagccaa cttcccggac ccggtagacc gtgcagccca ggaagaagag ttcagcctcg
     4201 aactgcgtaa ccgcgatcgc gagcgtaagc tgatcaaaaa gatcgagaag acgctgaaaa
     4261 aagtggaaga cgaagatttc ggctactgcg aatcctgcgg tgttgaaatt ggtattcgcc
     4321 gtctggaagc gcgcccgaca gccgatctgt gcatcgactg caaaacgctg gctgaaattc
     4381 gcgaaaaaca gatggctggc taattacagc cgttccatca cgtttaccac aggcgggaaa
     4441 tgctcccgcc ttattttttg ttcaaagaga tgacagacac acagtatatt ggccgcttcg
     4501 ccccctctcc ttccggcgag cttcattttg gctctctgat cgccgcgctc ggcagctatt
     4561 tgcaggctcg cgcccggcaa ggtcgctggc tggtacgcat agaagatatc gacccgcctc
     4621 gtgaagttcc cggtgccgca gaaactatcc tgcgccagct ggaacattac ggtctgcact
     4681 gggacggcga tgttctctgg caatcgcaac gtcaccacgc ctatcgtgaa gcactcgcct
     4741 ggttacatga acaaggacta agttattact gcacctgtac gcgtgcgcgt attcaaagca
     4801 ttggcggtat ttacgacggt cattgccggg tgttgcatca tggaccagac aatgccgcag
     4861 tgcgtatccg ccagcagcat ccggtcacgc aatttactga ccagctgcgc ggcattattc
     4921 acgccgacga aaaactggca cgggaagatt ttatcattca tcgccgtgat gggttgttcg
     4981 cctacaacct ggctgttgtg gttgatgatc atttccaggg cgttacagaa atagtgcgtg
     5041 gggctgatct gattgaacca acagtaaggc aaatctcgct gtaccagctt tttggctgga
     5101 aagtgccaga ttacattcat ctgccgctgg cgcttaatcc acaaggcgct aaactttcca
     5161 agcagaatca tgcgcctgcg ttgccgaaag gcgatccacg cccggtacta atcgcggcac
     5221 ttcaatttct ggggcagcag gcagaagcac actggcagga tttcagcgtc gagcaaatcc
     5281 ttcagtcagc cgtcaaaaac tggcggctta ccgccgtgcc cgagtcggca attgtaaatt
     5341 caacattctc aaatgcgtca tgctgagcta tgattagccg ctattttttt gtcctgaatg
     5401 atgtttgaca ctaccgaggt gtactatttt tacccgagtc gctaattttt gccgcaaggt
     5461 gctaagccgc gaggaaagcg aggctgaaca ggcagtcgcc cgtccacagg tgacggtgat
     5521 cccgcgtgag cagcatgcta tttcccgcaa agatatcagt gaaaatgccc tgaaggtaat
     5581 gtacaggctc aacaaagcgg gatacgaagc ctggctggtt ggcggcggcg tgcgcgacct
     5641 gttacttggc aaaaagccga aagattttga cgtaaccact aacgccacgc ctgagcaggt
     5701 gcgcaaactg ttccgtaact gccgcctggt gggtcgccgt ttccgtctgg ctcatgtaat
     5761 gtttggcccg gagattatcg aagttgcgac cttccgtgga caccacgaag gtaacgtcag
     5821 cgaccgcacg acctcccaac gcgggcaaaa cggcatgttg ctgcgcgaca acattttcgg
     5881 ctccatcgaa gaagacgccc agcgccgcga tttcactatc aacagcctct attacagcgt
     5941 agcggatttt accgtccgtg attacgttgg cggtatgaag gatctgaagg acggcgttat
     6001 ccgtctgatt ggtaacccgg aaacgcgcta ccgtgaagat ccggtacgta tgctgcgcgc
     6061 ggtacgtttt gccgccaaat tgggtatgcg catcagcccg gaaaccgcag aaccgatccc
     6121 tcgcctcgct accctgttga acgatatccc accggcacgc ctgtttgaag aatcgcttaa
     6181 actgctacaa gcgggctacg gttacgatac ctataagctg ttgtgtgaat atcatctgtt
     6241 ccagccgctg ttcccgacca ttacccgcta cttcacggaa aatggcgaca gcccgatgga
     6301 gcggatcatt gaacaggtgc tgaagaatac cgatacgcgt atccataacg atatgcgcgt
     6361 gaacccggcg ttcctgtttg ccgccatgtt ctggtaccca ctgctggaga cggcacagaa
     6421 gattgcccag gaaagcggcc tgacctatca cgacgctttc gcgctggcga tgaacgacgt
     6481 gctggacgaa gcctgccgtt cactggcaat cccgaaacgt ctgacgacgt taacccgcga
     6541 tatctggcag ttgcagttgc gtatgtcccg tcgtcagggt aaacgcgcat ggaaactgct
     6601 ggagcatcct aagttccgtg cggcttatga cctgttggcc ttgcgagctg aagttgaacg
     6661 taacgctgaa ctgcagcgtc tggtgaaatg gtggggtgag ttccaggttt ccgcgccacc
     6721 agatcaaaaa gggatgctta acgagttgga tgaagagccg tcaccgcgtc gccgtactcg
     6781 tcgtccacgc aaacgcgcac cgcgtcgtga gggtaccgca tgacagtggc gtatattgcc
     6841 ataggcagca atctggcctc tccgctggag caggtcaatg ctgccctgaa agcattaggc
     6901 gatatccctg aaagccacat tcttgccgtt tcttcgtttt accgcacccc accgctgggg
     6961 ccgcaagatc aacccgatta cctcaacgcc gccgtggcgc ttgaaacttc gctcgccccg
     7021 gaagagttac tcaaccatac acagcgcata gaactgcaac agggtcgtgt tcgtaaagct
     7081 gaacgctggg gaccacgcac gctggatctc gatatcatgc tgtttggtaa tgaagtgata
     7141 aataccgaac gcctgaccgt tccgcactac gatatgaaga atcgtggatt tatgctgtgg
     7201 ccgctgtttg agattgcacc ggagctggcg tttcctgatg gggagacgtt gcgtgaagtc
     7261 ttacatacaa gagcatttga caaattaagc aaatggtaat ttatttatat tacgaatata
     7321 aatattttct gaaaatcata catcctctct ttccttaata aaaattttag tttaaacatt
     7381 tttgcagaaa cataatataa attcccgcag ataaatatca atcccatcgc cataccctaa
     7441 ccaattgcac ttgtgaattt atttttgctt aattttgaat taagaaaatt taatttgttc
     7501 aaaagccctt aaagtgcaca ataggcgtcc taatgaacaa tggttgtaaa ttttggtaac
     7561 aaatatcatt gctcattaat atctagcatc tgcttataac atttgtgtca tgaatagtca
     7621 ttcttcaaac cagtagtctg actaatggaa ttttattcaa ggatttttat gtctaaaaaa
     7681 ttaggttttg ccctgtctgg tcttatgctg gcaatggttg caggtactgc ttctgctgat
     7741 atggatggtg gtcagttaaa tatcagtggt ctggttgttg ataacacctg tgaaacccgc
     7801 gttgacggcg gtaataaaga tggtctgatc ctgctgcaaa ctgcaactgt tggtgaaatc
     7861 gatgctggcg tgctgaatga caccgttggc gctaaagcca aacctttcag catcaccgtt
     7921 gactgcagca aagcaaatcc gaatccaggt agtacagcta aaatgacctt cggttctgtt
     7981 ttctttggta atagcaaagg aacgctgaac aacgacatgt ctattaacaa cccgtctgac
     8041 ggcgtgaata tcgctctgca caatattgat ggttccacta tcaaacaggt tcaaattaac
     8101 aaccctggcg atgtgtatac taaagccctg gatgcaacga caaaatctgc tgtttatgat
     8161 tttaaagcgt cttacgttcg tgccgtcgca gaccaaacag caactgctgg ttatgtaaaa
     8221 actaacactg catacaccat tacttatcag taagtattca ccaattaaat tgattgaatg
     8281 aatatacagg gaatagtgtt aataatttct atttttatat tattccctgt tttaattaac
     8341 tctgtcaggg atggtttatg ttttttaaca ccaaacatac aacagcttta tgcttcgtaa
     8401 cctgtatggc ttttagttca tcgtctattg cggacattgt catttcgggt actcgcgtaa
     8461 tatataaaag cgatcaaaaa agtgtcaacg tacgtctgga aaataaaggg aataacccgt
     8521 tgcttgtcca gagttggtta gatactggcg atgacaacgc tgagcctggc agtattacag
     8581 tcccttttac tgccacgccg ccagtatcgc gtattgatgc caaacgtggg caaacaatca
     8641 aattaatgta cacagccagc acctcactgc ctaaagacag agagagcgtg ttctggttta
     8701 acgtactgga agttccacca aaaccagatg cagaaaaggt cgcgaatcaa agtctgctgc
     8761 aactggcatt tcgcacacgt ataaaacttt tctatcgccc ggatggattg aagggaaatc
     8821 cctctgaagc cccgttagcc ctgaagtggt tctggtcagg ttcagaaggt aaggcgtcat
     8881 tgcgagtgac caatccaacc ccttactacg tctcttttag cagtggtgat ttagaagcta
     8941 gcggtaaacg ctatccgatt gatgtgaaaa tgattgcacc atttagtgat gaggtcatga
     9001 aagtcactgg ccttaatggc aaagcaagtt ctgcaaaagt acatttttac gccattaatg
     9061 attttggtgg cgccattgaa ggtaatgcca gtctgtaatc aggcaaggat ataattccgc
     9121 aggaagcata acgtgactat aaaaccaact aaaaattatc ataatcatct gacccgtatc
     9181 gctacgtttt gcgcgctgct gtattgcaat agtgctttct gtgctgaact tgttgaatat
     9241 gaccatacct tcctgatggg gcagaatgcc tcaaatattg atctcagccg gtatagtgaa
     9301 ggtaaccctg ctataccggg tatgtacgac gtcagtgttt atgtcaacga tcaacctatt
     9361 attaaccaaa gcattacatt tattgaaatt gaaggaaaaa aaaacgcaca agcctgtatc
     9421 acacttaaga atttattgca gtttcatata aattctcccg atataaataa cgaaaaagcc
     9481 gttctgcttg ccagggatga aacactcggc aattgcctta atttgacgga aattatccct
     9541 caggcttctg ttcgttatga cgttaacgag caacgtctgg atatagacgt tcctcaggca
     9601 tgggtaatga aaaattacca aaactatgtt gatccatcgt tatgggaaaa tggcattaat
     9661 gcggccatgt tgtcatacaa cctcaacgga tatcatagtg aaactcctgg tcgaagaaat
     9721 gacagtattt atgctgcttt taacggtggg atgaatttag gtgcatggcg actgcgtgcc
     9781 tcaggcaact acaactggat gaccgattct ggcagtaatt atgattttaa gaatcggtat
     9841 atacagcgtg atatcgcctc gctgcgttct caactcattc ttggtgagtc ttatacaacg
     9901 ggcgaaacct ttgattctgt cagtatccgc ggcattcgtt tatacagcga cagccgcatg
     9961 ctgcctccga cattagcgag ttttgcgcct atcattcatg gcgttgctaa taccaacgcc
    10021 aaagtcacta ttacgcaagg tggctataag atttatgaaa cgactgtgcc gccaggcgct
    10081 ttcgtcattg atgatctgag tccgtcaggt tacggcagcg atcttattgt tactgtcgaa
    10141 gaatccgatg gctcaaagcg gacattctcg caaccttttt catcggtcgt tcaaatgtta
    10201 cgccctggcg ttggacgttg ggatattagc ggcggccagg tcttaaaaga tgatattcag
    10261 gatgagccta atttatttca ggccagctac tactacggtc tgaataacta tctaacgggt
    10321 tatactggta ttcagattac cgataataac tataccgctg gtttgttagg tcttggtctg
    10381 aatacttcag tcggtgcatt ttctttcgat gtgactcact ccaatgttcg tataccggat
    10441 gataaaacat atcagggaca aagttatcgt gtttcctgga acaagttatt cgaagaaaca
    10501 agtacttcac tgaatatcgc ggcctatcgc tattcgacac agaattacct tggtcttaat
    10561 gatgcactaa ctctaattga tgaagtgaaa catcccgaac aagatcttga accgaaatcc
    10621 atgcgtaatt actcgcgcat gaaaaatcag gttacggtca gtattaacca accgctaaaa
    10681 tttgagaaaa aagattacgg ttcattttat ctttccggaa gttggtccga ttactgggct
    10741 tccggacaaa atcgtagcaa ttactctatt ggctacagta acagtgcatc ctggggcagc
    10801 tacagtgtca gtgcccagcg ttcatggaat gaagacggcg acactgacga tagcgtttat
    10861 ctcagtttca ccattcctat tgaaaaatta cttggcactg aacaacggac ttcaggtttc
    10921 cagagtattg atactcaaat gagcagtgac tttaagggta ataaccaact caacgtcagt
    10981 agcagtggct atagtgataa cgctcgcgtc agttatagcg taaatactgg ctatacgatg
    11041 aataaagcca gcaaagattt gagttatgtt gggggttatg ccagctatga atcaccgtgg
    11101 ggaagcctgg caggttcagt ttctgcaaat agcgataaca gtcgtcaagt gtctctcagc
    11161 accgacggtg gttttgtatt gcatagcggc ggcctgacct tcagtaatga cagttttagc
    11221 gactccgata cactggcggt agttcaggct ccaggtgctc aaggagcgcg aataaattat
    11281 ggcaacagta ctatcgatcg atggggttat ggtgtcacca gcgctctttc tccttatcat
    11341 gaaaaccgta tcgcgctgga tatcaacgat cttgagaacg atgttgaatt aaaaagtacc
    11401 agtgcagtag ctgtaccgcg tcagggttca gtcgtctttg ctgattttga aaccgtgcaa
    11461 gggcaatcag ccattatgaa catcacacga agtgatggta aaaatattcc atttgctgca
    11521 gatatttatg atgagaaagg caatgtcatt ggtaatgttg gacagggtgg acaagcattt
    11581 gttcgtggta ttgagcagca gggaaatatc agcattaaat ggctcgaaga aagtaaaccc
    11641 gtaagttgtc ttgcgcatta tcaacaaagt tcagaagcag aaaaaatagc gcaatctatt
    11701 attctgaatg gaattcggtg tcagattcag taactacaag ggacgtcaaa tgataaaaat
    11761 aacgccacat aaaatagcga tactgatggg aatattatta tcaccatcag tatttgcaac
    11821 ggatattaat gtagagttta cagccactgt caaagcgaca acctgtaaca tcacacttac
    11881 tggtaataac gtcacgaatg atggcaataa taactacaca ttgagaatcc ctaagatggg
    11941 tctggataag atcgcgaata aaacgacaga atctcaggct gattttaaac tggttgctag
    12001 tgggtgcagc agtggcatca gttggattga taccactctg accggaaatg catcatcaag
    12061 ctcacctaag cttattatac cgcagtctgg tgattcatct tcgacgacaa gtaatatcgg
    12121 tatgggtttc aaaaaacgga ctactgatga tgccactttc cttaaaccta acagtgcgga
    12181 aaagatacgc tggagcacag acgagatgca gcccgataag ggtcttgaaa tgaccgttgc
    12241 gctacgtgaa acagatgcag ggcaaggcgt accggggaat tttcgtgcac tggcaacgtt
    12301 taatttcatc tatcaataag aaggataatt aagatgacat ttaaaaattt acgttatgga
    12361 ttatccagca gtgtcgtttt ggctgcctca ttgttcagcg taccctctta tgcggcaaca
    12421 gacagcattg ggctgaccgt gattactacc gtagaaatag gtacttgtac tgctacatta
    12481 gtaaatgact ctgatcagga catttctgtt gttgactttg gcgatgtata tatttctgaa
    12541 atcaatgcca agaccaaagt taaaacattc aaactcaaat tcaaagactg tgcgggtatc
    12601 cccaataaaa aagcacaaat aaaattaacc aagcgagcca catgcgaagg tactgctaat
    12661 gacggtgcgg ggttcgcaaa tggatccaca gccgcagata aagcaagtgc cgtcgccgtt
    12721 gaagtctgga gcaccgaaac tccggcaaca gggagcgcaa cacaatttag ttgtgtgaca
    12781 ccagcatctc aagaggtcac tatctccaat gcagccaacg cggttgttta ttatccgatg
    12841 agtgctcgac tggtcgtcga aaaaaataaa accgtaagca atgtcactgc gggtaaattt
    12901 tctgcacctg ccacatttac agtaacctat aactaacaga ctgacagatc caggaactat
    12961 atatgcatcc cactcaacgt aagctgatga agagaataat tctgtttctg tcactacagt
    13021 ttcccattac ctatccagcc atcgctgggc aggatattga ccttgttgcc aatgtaaaaa
    13081 acagcacttg caaaagcgga atcagtaacc agggtaatat tgatcttggc gtcgttgggg
    13141 tgggatattt ttcagataat gttactcctg aaagctatca accaggtgga aaagagttca
    13201 ctgtcacagt atcagactgt gcattacagg gaactggcga tgtgcttaat cagttacata
    13261 ttgattttag agcccttagc ggtgtcatgg ctgcaggttc aaggcaaata tttgccaatg
    13321 aagtcgcaac cggggctaaa aatgtcggtg tcgttctctt ttccattcag gacccgacca
    13381 atatattcaa tgttattagt tcagcaggaa attctcgttc cgtgtatcca gtaatgactt
    13441 ctgccctgca taattcttca tggaaatttt atgccagaat gcaaaaaatc gatcccgcgc
    13501 tggatgtcat atctggccag gtaatgagtc atatattagt tgatgtgtac tacgaataat
    13561 agatcaaaac aaataactgc gactgttaca gcagtagatg gtggagtgat aatgaagaaa
    13621 ttcttcagac attttctttt tttaattctg tgcttgagtt gttataccgc gagtgctggc
    13681 actgatgata acgtaggata tattgttggg aatagttacg gcgttggtcc gtcagatcaa
    13741 aaatggagag aaacggggcc gaatggcgat gctactgtca tattcagata tgcaacaagt
    13801 acaaataatt tagttttcta caaaccgacg cagcttggac ctacaggtgt aaaattacag
    13861 tggagtcagt tagataccgc ttctggtggt ggttttcttt attgcaacag aagtgatagc
    13921 acaagtggta gcgcaatgcg tattgaaaat gcaatggttg actcaggtaa aatgtatggc
    13981 tcccataaat tatttaatac atcagttcct ggtttgtatt acacattatt aatttcaaac
    14041 atgtggtcag cttacggtac cgtaactaac gttagttcac ctgggatata cattggtgac
    14101 tctgcagaac aatatttttc gtggtataat ccaagcgaag acgtgttata ttggagttgc
    14161 aataatgcga atagcacccg taaatactgg gctgtaggtg gtatttatca gacccttaca
    14221 attgaattct atacagatac aaactttgat ccaacagtaa ctcaacagat caaattatcc
    14281 agctcatcaa attatctgta ttcatttaaa gcctatggcg caggccaagg tataaatgag
    14341 catagttatt ttatcaaaat cgatttcgat ctgctcaatg tcaagttgac taatcccact
    14401 tgttttaccg ctatgcttag tggaacttct gtgactggtt ctacggtaaa aatgggtgaa
    14461 tatagtgcag aacaaatcag aaacggtgcc acaccggttc cttttgatat ttcacttcaa
    14521 aactgcgttc gtgtgactaa tattgagaca aaattagttt caacaaaggt tggtactgaa
    14581 aacgggcaac tccttggtaa tactctcaca ggtaatgacg cagcaaaagg agtcggtgta
    14641 ctcatagaag gtttagcaac tagtaaaaat cctctaatga cattgaaacc taatgattca
    14701 aattctgttt ataaagatta cgacccgaga ggcaaagacg atacaacagg aggagtttac
    14761 ccggatcaag atacaggtat aacataccct ctccatttcc aggccacgct acaacaggat
    14821 ggaactatac caatagaagc tggtgagttt aaagccacca gtactttcca ggtaacctac
    14881 ccttaataag tccaccgcac cgccatcatc tggcggtgcg gtaattgata aatcattgcc
    14941 cccaccaatg tgactgataa aatgccaggc aacacgacat catttatcag gacacgttat
    15001 gaaaccgacc accattgctt cacttcagaa atgtaagcag gataaaaagc gtttcgcgac
    15061 catcaccgct tacgactaca gcttcgcgaa actatttgct gatgaaggac ttaacgtcat
    15121 gctggtaggc gattcgctgg gcatgacggt tcagggccac gactccaccc ttcccgttac
    15181 cgtcgaggat atcgcctatc ataccgccgc cgtacgtcgc ggcgcgccaa actgcctgct
    15241 gttggctgac ctgccgttta tggcgtatgc cacgccggaa caagcctttg aaaacgccgc
    15301 aacggttatg cgtgccggtg ccaatatggt caaaattgag ggcggtgagt ggctggtcga
    15361 aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt ggtcacttag gtttaacccc
    15421 acagtcagtg aatattttcg gtggctacaa agttcagggg cgcggcgatg aagcgggcga
    15481 tcaactgctc agcgatgcat tagccttaga agccgctggg gcacagctgc tggtgctgga
    15541 atgcgtgccg gttgaactgg caaaacgtat taccgaagca ctggcgatcc cggttattgg
    15601 cattggcgca ggcaacgtca ctgacgggca gatcctcgtg atgcacgacg ccttcggcat
    15661 taccggcggt cacattccta aattcgctaa aaatttcctc gccgaaacgg gcgacatccg
    15721 cgcggctgtg cggcagtata tggctgaagt ggagtccggc gtttatccgg gcgaagaaca
    15781 cagtttccat taaggagtca cgttgtgtta attatcgaaa ccctgccgct gctgcgtcag
    15841 caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc tggtgcctac tatgggtaat
    15901 ctgcacgatg gccatatgaa gctggtcgac gaagccaaag cccgcgccga tgtggtcgtc
    15961 gtcagtattt tcgttaaccc gatgcagttc gaccgcccgg aagatctggc acgttatcca
    16021 cggaccttgc aggaggactg cgagaagcta aacaaacgta aagtggattt agttttcgcc
    16081 ccttcggtaa aagagatcta cccgaacggt actgaaaccc atacttacgt tgacgttccc
    16141 gggctttcga ccatgctgga aggcgcgagc cgtccggggc attttcgcgg cgtttcgact
    16201 atcgtcagca agctgtttaa cctggtccag ccggacatcg cctgcttcgg tgaaaaggac
    16261 tttcagcaac tggcgctgat ccgcaaaatg gttgccgata tgggcttcga tattgagatt
    16321 gtcggtgtgc caattatgcg cgccaaagat ggccttgcgc tcagttcccg taatggttat
    16381 ctgacggcgg aacaacgcaa aattgcgccc ggtctgtaca aagttttaag ttcaattgcc
    16441 gacaaattgc aggctgggga acgggatctc gatgaaatta ttaccattgc ggggcaagaa
    16501 ctgaatgaaa aaggcttccg cgccgatgat attcagattc gcgatgccga cacattgctg
    16561 gaagtttctg aaaccagcaa acgggcagta attctggtag ccgcctggct tggcgatgct
    16621 cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact taactggcgc tacggctgat
    16681 ggcgccagtt attaatttac cccacgtcat ctgtcataac acaggtaact taaataaggt
    16741 taattacctt atcaatctca gcaagaggta aatttgccat ctctgcaacg tcttcccgag
    16801 acattccttt acttagaaga cgcagggcga attcttgcct ttcctcctgt cttccctgct
    16861 gaatcccctt ctcaatccct ttctcttcaa accactgcgc cagcgtcatc atagactccc
    16921 ctcccgtttc cctgtctctc agcacaccat aaaacaaatc cgcttgttca gtatgaccgc
    16981 gttgcagcat atagttttgc atggcaacta actgacttcc gctagtgtac ccttcgtcaa
    17041 tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg ctggcgaata tgtttttgca
    17101 gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt gtgatggtga
    17161 tatccaccag cgggaaagga ctgttataga cgcgtcgtgc cagctccggc gagtaaaata
    17221 tatcaaacca gcacattgag agcggataag gtgtggcctc gccctgataa aacagtatcg
    17281 gcaccaccag cggcagctta tcgtgatccg cctccagatg ccggtgcatg gcagcaatag
    17341 aataacgcat catgcgaaag gccattttct tatccggctt gctttggtgt tcaatcacaa
    17401 catgcagata accgggattg ccctgcattt gcacggaata gagcacgtcc gtgctgtgtc
    17461 ctttcaggct ctcttcaatg aaactccccg actctaaatg aagcgtgttg aggtcacaaa
    17521 gttctcgtaa ttccactggc aaatgtatct ccagaaagtc gcgagccgtc tccgcatgca
    17581 ttaaaaattg ttaaataccg cgtcatgcgg tgtggtactc ggtgcatcca tcttccctgt
    17641 cttccgtgtt gtcttgcgtg aaggcagtat ccagtcaagg cgcgccgtcg tcagccgcct
    17701 ctttagtgtt taccgagcag cgttcagaga aatttcaatt cgttacaggc gatacatggc
    17761 acgcttcggc gcggtacgca aactgctgac gcaaatgcca tcagatgagt agacactcat
    17821 caaaaatcgg gcaatactgc gtgagaattt cctcaaagca ggcaccgcct gcttcgttaa
    17881 cgacagggta gaaaggtaga agttatgatt cgcacgatgc tgcagggcaa actccaccgc
    17941 gtgaaagtga ctcatgcgga cctgcactat gaaggttctt gcgccattga ccaggatttt
    18001 cttgacgcag ccggtattct cgaaaacgaa gccatcgata tctggaatgt caccaacggc
    18061 aagcgtttct ccacttatgc catcgcggca gaacgcggtt cgagaattat ttctgttaac
    18121 ggtgcggcgg cccactgcgc cagcgtcggc gatattgtca tcatcgccag cttcgttacc
    18181 atgccagatg aagaagctcg cacctggcga cccaatgtcg cctattttga aggcgacaat
    18241 gaaatgaaac gtaccgcgaa agcgattccg gtacaggttg cttgattgtt atccctgcgg
    18301 ctggttactc accagccgcg acatcgtctc cagcgaatcc gttcttaaga tataaagtcg
    18361 ttttagtaac aacggattat cccccggttt tactttgcct ttcatggttg tcaccgccag
    18421 gtgaaatccg gcatcgtttg ctgccttcac ggctttgtca ttaaatccgc caaacggata
    18481 cgaaagatac cagacatgcg gattaaattg cgccagagcg cggcgtgaac gtgcaaaatc
    18541 aaacagaata ttgtgctcac tacggctcag taatatgggt cggcgatagc catccacccg
    18601 atgcaaaaaa tgggtatgtg actgaaaatc aaaaacatcg cgaatttcgt taagctcaga
    18661 aacgctcata aattgcagcg attttgggtt ccacttctgc gggtgacgtt tgatgcgcga
    18721 ggtaacaaca aacgccgtcg ccttcatgcc atattgtttc aatacaggat acgcatagcg
    18781 gctcaccgac ttgaggccat catcaaaggt aatcaccacc gctcgcgcag ggagattgat
    18841 cttattcttc acgtaacctt ccagctgcgc catgctcagt gtcgcgtatc ccctgtcgcg
    18901 cagccaggcc atctggttat tgaaagcgcg taccgaggtg gtcgtcgaag tatggcgaaa
    18961 acgggtgttt tcttcatcgc gcagaatatg gtgataggtt agcaccggca ggccattatc
    19021 gggttgggca tccagcgcgc tgatataggc gagtcgatcg ccaatacgga tctgatacca
    19081 ggtttgattt aacctgtctt tcagtttatg caaaatcggg tagcgcaaat tgtccgccag
    19141 taccccaaat ggcgcactgc ccgcactcgg cgcgttatag accggcgtat ctttccaggt
    19201 aattaagttc tgattactca gcggcttgtt gaggtcaccc aaaccgtctt caactttttg
    19261 tcgcccctga actggctcga gatgcccttt atcgataaac cctttaccaa agccaaaatt
    19321 aaatgcgtaa taacttgcgg cagtgggctc cacggcaatg atttgcccgg cccgaatatt
    19381 ccccacggtc accatcttgt cgccaatttg cgcccagatc gcagcatttt cgatggtttg
    19441 catataacgg gcaggtaacg cggcactgac acttgcggta aacagcatca gcaggagaat
    19501 aacagcttgt ttgtacatga aaaaataacc gagggtgaaa caacgctttt attgtagcaa
    19561 aagcgctata agaatcgtgg gttaatactc atacaaatta tgtttgagga cgaaaggcgg
    19621 atttttttgt tgttggtgcc aaagactact cacctccggc ccacccagtt cgacaatacg
    19681 ctcgcgaaac tctgaactag tcatggtttc acggcaagcc atcatctgtt caattaacgg
    19741 taacgtgaca ccagaaatca cttcgcaacg ggagtgtttg tggcttaata atgaagccac
    19801 gcggtacggt ggcgctccgg ctatatcggt taagaagatg acaccctcac cagagtccgc
    19861 ttcatgcaga gcatcgcaca tcatgcggct gagcatatta gagcttaacc cacgccagaa
    19921 attcacggcc cggcactgaa gaagcgcccc atgttttttc tccagcgcat ccagtatctc
    19981 ttgcgcccgg tcatcgtgac aggtaattac ccaacctaac atcgccttcc tccttagccg
    20041 aaaatcagtc taccctgacc attgttgata aagggtgata gccgtcaaaa ctcctctccc
    20101 ccaaatccag aggagaggaa atagccttag ctacgcaaac cacgtccacg ttggatcagc
    20161 gaccaacaga tcaaataaaa cgccacaata aagaccacca gtacgccaaa ggtagtgacc
    20221 agcggaacat cattgatacc gaggaagccg tagcggaaac cactgatcat ataaacgatt
    20281 gggttcaggt gcgacagccc ttgccagaac ggcggcaaca aagtcagtga gtaaaagacc
    20341 ccacccaaat acgtgagtgg cgttaacaca aaggttggca ccaggctgat gtcatcgaac
    20401 gttttggcaa acacaccgtt cagcaaaccc gcaagggaga acaacaccgc cgtgagcacc
    20461 agggttaagg caacgaatac ccacgaatgc acctgaaacg gcacaaaaaa cagtgaaatt
    20521 gccgtcacca gaatgccaac aaacagacca cgcgccacgc caccgccgac atatccggca
    20581 ataatgacgt gagtcggaac cggcgctacc agcagctctt caatattacg ctggaacttg
    20641 gcaccaaaaa atgatgacgc aacgttggcg taggcattgg tgatcaccga catcatgatc
    20701 agccccggta cgatgaactg catatagctg aagccatgca tatcgccaat acgcgaacca
    20761 atcaggttac cgaagataat aaagtaaagg gtcatggtga tgactggcgg caccagcgtc
    20821 tgcacccaga tacgcataaa gcgatggatc tctttcgccc agatgctttt tagcgccacc
    20881 cagtaaagat gcatcatgcg cgatctcctt gtttttcatt aaccagtgaa acaaacagct
    20941 cttccagacg gttagctttg ttacgcatac ttaatacctg aatgccctgc tcacttaact
    21001 gcgtaaatac gctgttgatc ccctgctcac gcagcacttc aacttccagc gtcgcggtat
    21061 cgaccagtcg atactgatag ccatcgagct tcggtaacgg gcttttcggt gcgagatcga
    21121 gaataaaggt ttccgatttc agcttcgcca gcagcgcctt catcgaggta ttttccacca
    21181 gctcaccgtg ttgaataatg ccgatattgc ggcacagcat ttctgcttct tccaggtagt
    21241 gtgtggtgag aatgatggtg gtgcctttgt cgtttaaatc cttcaaaaag ccccacattg
    21301 agcggcgaag ttcaatatcc acgcctgcgg tcggttcgtc gagaatcagt agtttaggtt
    21361 catgcattaa cgcacgggca atcattaaac ggcgcttcat cccgccagat aacatacgcg
    21421 cacgttcgtt gcgttttccc catagatcga gttgtttaag atacttttcg ctgcggatgt
    21481 acgcttcttt gcgctccacg ccatagtacc ctgcctgatt caccacaatt tgctgcacgg
    21541 tttcaaacgg gttgaagtta aattcctgcg gcaccagtcc caactgacgt ttagcgttca
    21601 cgacatcctt ctcgagatcg taaccaaata cgctgacccg cccggaggtt ttatttacca
    21661 gagagctgat aataccgata gtggtcgatt tcccggcccc gttcggcccg agaagcgcat
    21721 aaaaatcacc cgcttcgacc tgcaaatcta tcccacgaag cgcctgaacg ccgcctggat
    21781 aggttttttt aagctgttga agttccagtg caatggtcat aaatttttac ttaccttacg
    21841 ttcttacact ttatctgtgg tttaaatcgt cccggagttg ccctatatta gccaaacgta
    21901 attatttggt tacaggtcgt taacctccat gaaagacata gatacactca tcagcaacaa
    21961 tgcactatgg tcaaaaatgc tggtggaaga ggatcccggg ttttttgaga aactggcaca
    22021 agcgcaaaaa ccgcgctttc tatggattgg atgttccgac agtcgcgttc ctgcagaacg
    22081 tttaaccggt cttgagccgg gcgaactctt tgttcaccgt aatgttgcta acctggtcat
    22141 tcacactgac ctgaactgcc tttccgtggt tcagtatgca gtggatgtac tcgaagttga
    22201 acacattatt atctgtggcc actacggttg cggcggcgta caagccgcag ttgaaaaccc
    22261 ggaactgggg cttatcaaca actggctgct gcatatccgc gatatctggt tcaaacatag
    22321 ctcattgctc ggcgaaatgc cgcaagagcg ccgtctggat accttgtgtg aactgaacgt
    22381 catggaacag gtgtataacc tgggccactc caccattatg caatcagcgt ggaaacgcgg
    22441 gcagaaagtt accattcacg gctgggccta cggcattcac gacggcttgc tgcgtgatct
    22501 ggatgtcaca gcgaccaacc gcgaaaccct tgagcaacgt taccgtcacg ggatttccaa
    22561 cctcaagctg aaacacgcca accacaaata aaaatgccat gccggatgca acacatccgg
    22621 caacttcaca cttactcgtc cagcagaatc actttgccga tatacggcag atgacggtaa
    22681 cgctgtgcgt aatcaatgcc gtaacccacc acaaactcat ccgggatcga gaaaccgata
    22741 aattctaccg ggacgttcac ttcacgacgg gacggtttat ccagcagcgt acaaatcgcc
    22801 agcgacttcg gttcgcgcag gcttaagatc tcacgcactt tcgacagtgt attccccgag
    22861 tcgatgatat cttcaacaat cagcacgtcc ttgccacgga tatcttcatc cagatctttg
    22921 aggattttca catcacgggt ggtggacatg ccgctaccgt agctggaggc ggtcataaag
    22981 tcgacttcat gagatacctg aacttcacgg cacaggtccg ccataaacat aaatgagcca
    23041 cgcagcagac caaccagcac catatcgctg ccgctgtctt tgtaacgctc agtaatctga
    23101 cgacccagtt cggcgatacg cgctttaatc tccgcttcgg ggatcattac ttctacagta
    23161 tgtttcatat ctctaaccat atgatttaaa aacaaatcac ttcgcacggg caggttttgg
    23221 caccgcaaca aagcgcaaga cacgcagtat accagtaaaa ctattcattc gcggaatctg
    23281 ttaataaagc tcgaattgtg atgacgatca cacatgttaa aacctataat taattgctac
    23341 taaaatatta atgaattgaa atggtgtctc tttatggcaa ttaacaatac aggctcgcga
    23401 cgattactcg tcacgctaac agcccttttt gcagcgcttt gcgggctgta tctactcatt
    23461 ggcggaggct ggctggtcgc gattggcggc tcctggtact accctatcgc tggtcttgtg
    23521 atgctcggcg tcgcctggat gctgtggcgc agtaaacgtg ccgcgctttg gctgtacgca
    23581 gccctgctgc tcggcaccat gatttggggc gtctgggaag ttggtttcga cttctgggcg
    23641 ctgactccgc gcagcgacat tctggtcttc ttcggcatct ggctgatcct gccgtttgtc
    23701 tggcgtcgcc tggtcattcc tgccagcggc gcagttgccg cactggtggt ggcactgctg
    23761 attagcggtg gtatcctgac ctgggccgga tttaacgatc cgcaggaaat caacggcacc
    23821 ttaagcgcca atgccacacc tgcggaagct atctcacccg ttgccgatca ggactggcct
    23881 gcctatggtc gcaatcagga aggtcaacgc ttttcacctc tgaaacaaat taacgccgat
    23941 aacgtccata atctgaaaga agcctgggtg ttccgtactg gcgatgtgaa gcagccgaac
    24001 gatccgggcg aaatcaccaa tgaagtgacg ccgattaaag tgggcgacac cctttacctg
    24061 tgtaccgctc accagcgcct gtttgcgctc gatgccgcca gcggcaaaga gaaatggcat
    24121 tacgatcctg agctgaaaac taacgagtct ttccagcacg taacctgccg tggcgtctct
    24181 tatcatgaag ccaaagcaga aacggcttcg ccggaagtga tggcggattg cccgcgtcgt
    24241 atcattcttc cggtcaatga tggtcgactg attgcgatta acgctgaaaa cggcaaactg
    24301 tgcgaaacct tcgccaataa aggcgtgctc aatctgcaaa gcaatatgcc agacaccaaa
    24361 ccgggtctgt atgaaccgac ttcgccaccg attatcaccg ataaaaccat cgtgatggcc
    24421 ggttcagtta ccgataactt ctcaacccgc gaaacgtctg gcgtgatccg tggttttgat
    24481 gtcaacaccg gggagctgct gtgggctttt gatcccggcg cgaaagatcc gaacgcaatc
    24541 ccgtctgacg aacacacctt tacctttaac tcgccaaact cctgggcacc agcggcctat
    24601 gacgcgaagc tggatctggt ctatctgccg atgggcgtga ccacgccgga tatctggggc
    24661 ggtaaccgca caccggaaca ggaacgttat gccagctcga ttctggcgct gaatgccact
    24721 accgggaaac tggcgtggag ctaccagacc gttcaccacg acctgtggga catggatctt
    24781 ccggcacagc cgacgctggc ggacatcacc gttaatggtc agaaagtgcc agttatttac
    24841 gctccggcga aaaccggcaa catttttgtg ctcgatcgtc gcaatggcga actggtggtt
    24901 ccggccccgg aaaaaccggt gccgcaaggt gctgcgaaag gcgattacgt tactccgacc
    24961 cagccgttct ctgaactgag cttccgtccg aagaaagatt tgagcggtgc ggatatgtgg
    25021 ggagccacca tgtttgacca actggtgtgc cgcgtgatgt tccaccagtt gcgctatgaa
    25081 ggcattttca cgccgccatc tgaacagggc acgctggtct tcccgggtaa cctgggaatg
    25141 tttgaatggg gcgggatttc cgttgatcca aatcgtgaag tggcaattgc caacccaatg
    25201 gcactgccgt ttgtttcgaa actgatccca cgcggtccgg gtaacccgat ggagcagccg
    25261 aaagatgcca aaggcacggg tacggaatcc ggcattcagc cacagtacgg tgtaccgtat
    25321 ggtgtcacgc tcaacccgtt cctctcaccg tttggtctgc cgtgtaaaca gccagcatgg
    25381 ggttatatct cggcgctgga cctgaaaacc aatgaagtgg tgtggaagaa acgtattggt
    25441 acgccgcagg acagtatgcc gttcccgatg cctgtaccgg tgccgttcaa tatgggtatg
    25501 ccgatgctgg gcgggccaat ctccacggcg ggtaacgtgc tgtttatcgc cgctacggca
    25561 gataactacc tgcgcgctta caacatgagc aacggtgaaa aactgtggca gggtcgttta
    25621 ccagcgggtg gtcaggctac gccaatgacc tatgaagtga atggtaagca gtatgtggtg
    25681 atctccgcag gcggtcacgg ttcatttggt acgaagatgg gcgactatat tgtggcttat
    25741 gcgctgccgg atgatgtgaa gtaaccataa tgcccggaga aatccgggca tatttccgaa
    25801 tacggtcttt ttataccgta aaccctaaca tcatccccgt atcttcatgc tccagcagat
    25861 ggcagtgcgc catataagca tgttctttcg gtgcgtcgtg attaaacttc accagcactt
    25921 cactgacatt gccttctacc ttaacggtat ctttccagcc cgcgcgatga gccgctggcg
    25981 gtttgccatt ttctgacaag atacggaact gggtgccgtg aatatggaac ggatgcagca
    26041 tcatgtcgcc cacgccagag ataacccaac gttcgtactg ccctttcgcc gccgcaaaca
    26101 tcggcttatt catatcaaac gcctgaccgt tgattttatt ggcatggtgg aaatcgaact
    26161 tcccgccgtg gttcatatga ttcatattac cgtgccccat atggcccatc atctggctgt
    26221 gatccattcc agccatcgcc tgatcgccat atttctccat tagcatctgc atacccatca
    26281 tatcgagcat cgggtccata gaaagttgca gcttgcgtac cgtcagccct tccagcgaag
    26341 gtaacgcagg caggctactt aatgtgtctg gcaaagcacc ggaggcacta atagcaatcg
    26401 gctgaatccg cattaccgga tgcggcttgt caaacggcgc aatcgccatc cccatctggc
    26461 tgaccggtag cgtcaccagg tcaaagggtt tgttgtcgtt aacctccacc agcacttcaa
    26521 aacgctcgcc catcagcacc ggcagctcgc tcaccttcac cggttcaggt agcagaccac
    26581 cgtcgctggc aatcacatac agcgggcgat tgtcgctggt ggcgaaattg agcgagcggg
    26641 cattacagcc attgagcaaa cgcaggcgca gccaaccacg cggggcagcg tgttgtgggt
    26701 agattgcacc gttggtcagc agcgtatcgc caaaccagcc cacggcggcg gtcatcacat
    26761 ccagttgata atcaatctgc ccatcagcgt taaatttttt atcctgaaca atcaccggaa
    26821 catcatcgat accccactgt tttggcagca ttaatttcag gatctcgtca tcttcaatta
    26881 ccaccagccc agccaacccc atcgccacct gtcgcccggt cttgccatgt tgatgtggat
    26941 ggaaccagca agtagcggca ggttgatcaa cgttcaacgt caccgagcgc ttgccacctg
    27001 gcagaataat tccctgcggg ccgccgtcga cttcacccgg tacttccagc ccgtgccagt
    27061 gcaacgtcgt ctcttccgtc agttggttat agatatcaac cgttaccgct ttgccgcgct
    27121 gtaatttcac cgccggcccc agcagattgc cgttatagcc ccaggtagtt gcagttttct
    27181 cgccaaaggt agactgacct gcgccaatag ttaactgaat gcgattacgg gcatcggtcg
    27241 tgagcaaatc agggatcggt aacgttgggc gttccgccgc aaagactgcg cggctccaca
    27301 gcggcaaggc tgaagccaca cccagcgcga cggaatattt taagaaatca cgacgttgca
    27361 tagttatttc cttattctta agcaggcgaa acaaaatcaa acgttgagca tagtccttcc
    27421 ccttacggga aggtcaagcc gcagacacaa tttaatcgac aataatggtc gtcgcctcgc
    27481 tcgcagtgtg ctaacgttta tcttctttaa gcccctggta gaagcaatga agacgttttt
    27541 cagaacagtg ttattcggca gcctgatggc cgtttgcgca aacagttacg cgctcagcga
    27601 gtctgaagcc gaagatatgg ccgatttaac ggcagttttt gtctttctga agaacgattg
    27661 tggttaccag aacttaccta acgggcaaat tcgtcgcgca ctggtctttt tcgcccagca
    27721 aaaccagtgg gatctcagta attacgacac cttcgacatg aaagccctcg gtgaagacag
    27781 ctaccgcgat ctcagcggca ttggcattcc cgtcgctaaa aagtgcaaag ccctggcccg
    27841 cgattcctta agcctgcttg cctacgtcaa ataatccctg ataccttttt gtagaaatat
    27901 tgaccgtgca tccgcggtca atgttagcta ttatgttgcg cccttttttt acgggtgtta
    27961 acaaaggagg tatcaaccca tggccgaaaa aaaacagtgg catgaaacgc tacacgacca
    28021 gtttgggcag tactttgcgg tagataacgt tctgtatcat gaaaagaccg atcaccaaga
    28081 tctgatcatt tttgagaacg ctgcatttgg tcgcgtaatg gcgctggatg gcgtagtaca
    28141 aaccaccgag cgcgacgagt ttatctatca tgagatgatg acccatgttc cgctactggc
    28201 ccacggtcac gcgaaacatg tgctgattat cggcggcggc gacggtgcca tgctgcgtga
    28261 agtaacccga cataaaaacg ttgagtcaat cacgatggtg gaaatcgatg cgggtgtcgt
    28321 gtcgttctgc cgtcagtatc tgcccaacca taacgccggt agctacgacg atccgcgctt
    28381 taagctggtg atcgacgatg gcgtcaattt cgttaatcaa accagccaga cctttgatgt
    28441 cattatctcc gactgcaccg atcctatcgg tcccggcgaa agtctgttca cctcagcatt
    28501 ttatgaaggc tgcaaacgtt gcctgaatcc tggcggtatc ttcgtcgcac aaaacggcgt
    28561 ctgcttttta cagcaggaag aagccatcga cagccatcgc aaactcagcc attacttcag
    28621 cgacgttggc ttttatcagg cggcgatccc gacctattac ggcggtatca tgacttttgc
    28681 atgggcgaca gataacgacg ccttacgcca tctctcaacc gaaattattc aggcgcgttt
    28741 tctcgcttct ggcctgaaat gccgttatta caatccggca atccatacgg cagcttttgc
    28801 cttacctcag tatctgcaag acgcactggc ttcacagccg tcctaaggag aagataagaa
    28861 attgaaaaaa ctgaaactgc atggctttaa taatctgacc aaaagtctga gtttttgtat
    28921 ttacgatatc tgctacgcca aaactgccga agagcgcgac ggttatattg cttatatcga
    28981 tgaactctat aatgccaacc gtctgaccga aatcctgtca gaaacctgtt ccattatcgg
    29041 ggccaatatt cttaacatcg cccgccagga ttacgaacca cagggtgcca gcgtcactat
    29101 tctggtgagt gaagaaccgg ttgacccgaa actcatcgac aaaacagaac accccggccc
    29161 actgccagaa acggtggttg cccatctcga taaaagtcat atttgcgtac atacctaccc
    29221 ggaaagtcat cctgaaggcg gtttatgtac cttccgcgcc gatattgaag tctctacctg
    29281 cggcgtgatt tctccgctga aggcgctgaa ttacctgatc caccagcttg agtccgatat
    29341 cgtaaccatt gattatcgcg tgcgcggttt tactcgcgac attaacggta tgaagcactt
    29401 tatcgaccat gagattaatt cgattcagaa ctttatgtct gacgatatga aggcgctgta
    29461 tgacatggtg gatgtgaacg tctatcagga aaatatcttc cataccaaga tgttgcttaa
    29521 agagttcgac cttaagcact acatgttcca caccaaaccg gaagacttaa ccgacagcga
    29581 gcgccaggaa attaccgctg cgctgtggaa agagatgcgc gagatttatt acgggcgcaa
    29641 tatgccagct gtttaacggc tctggcggag ctcccaggct ccgccagatt tatttacttc
    29701 tgctgcacga aattacggta agccgccacg acctgcagaa aatcctcaac gccgcatagc
    29761 gacaggcttt cttcgtcgta gtagttcatc ccctcttcca tttcatcgcc agcgaattcc
    29821 agttgattgg cgcgaaccat cacctcttca ccgtccatcc acagagtgta ctcatgccct
    29881 gcccgttgcc aggaccgttc gctacctttc agtgcgtgcg ctgcttgttc cacttcatca
    29941 agcaaggcca ggttttcttt cacctcttca ttaaaccagt gcccgaccac ttcatgcccc
    30001 atggacatac gcacctttac cactccggta atatcgcgca gaaattcgta atccatagtg
    30061 tgttcctctg ctcccggcaa tgcgccgaaa tcgcattgcg ccattgatgt aattatcgca
    30121 gccttaccgg agaaaaacag cggaacggcg atggtttaga aataaaaaaa tgcccgggaa
    30181 tctcattcac cgggcattgt gtcgtttatg cgcagcgcgt gcgcttactt attacaccgc
    30241 agtctggaaa atcaccccat cggctttttc ggtgtactga gaaagctggt tgaagttcag
    30301 ataacgataa gtatcaacgg cagttttatc tacttgcgcc acgtaggtct ggtactcttc
    30361 cggcgtcggc agtttgccaa tcagcgccgc aacagccgcc agttccgcag aagccaggaa
    30421 gacattcgcg ccagtaccca gacggttcgg gaagttacgg gtagaggtgg aaaccaccgt
    30481 cgcaccgtct gctacacgcg cctggttacc catacacagg gaacagccag ggatctcgat
    30541 acgcgcacca ctcttaccga agacgctgta gtagccttct tcagtcaact gcgcggcgtc
    30601 catacgggtt ggcggtgcca cccacaggcg ggtcggcagt tggcctttgt gcgcatccag
    30661 cagtttaccc gcagcacgga agtgaccgat gttggtcatg caggaaccga taaatacttc
    30721 gtcgatcttc tcaccctgta ccgcagacag cggacgcgcg tcatccgggt cgttcggtgc
    30781 acagaggatt ggctctttaa tatccgccag atcgatgtcg atcactgccg cgtattcagc
    30841 atctgcatcg gcttccagca gctcaggatt cgccagccat ttttccatgc cctgaatacg
    30901 acgttccagg gtgcgacgat cgccgtaacc ttccgcgatc atccacttca gcaggacgat
    30961 gttagagttc aggtattcga tgatcggttc tttgttcagc ttgatggtac aaccagcggc
    31021 agaacgctcg gcggacgcat cggtcagttc aaatgcctgc tcaactttca gatccggcag
    31081 accttcaatt tccaggatgc ggccagagaa gatgtttttc ttgcctttct tctcaacggt
    31141 cagcagacct tgtttgatcg catacagcgg aatggcatgt accagatcgc gcagggtgat
    31201 gcccggctgc attttgcctt tgaagcgcac cagaacggat tccggcatat ccagcggcat
    31261 tacgcctgtt gcggctgcaa atgccaccag accagaaccc gccgggaaag agataccgat
    31321 cgggaaacgg gtatgggaat caccaccggt accgacggta tccggcagca gcatacggtt
    31381 cagccaggag tgaatgacgc cgtcacccgg acgcagcgac acaccgccac ggttcataat
    31441 gaagtccggc agcgtgtggt gcgtgttcac gtcaactggc ttcggatacg ccgcggtgtg
    31501 gcagaaagac tgcatcacca ggtcagccga gaagcccagg cacgccaggt ctttcagttc
    31561 atcacgggtc atcgggccgg tggtgtcctg ggaacctaca gaagtcattt tcggttcaca
    31621 gtacgcgccc ggacgaatgc ctttcacgcc acaggcacgg cctaccattt tttgcgccag
    31681 cgagaagccg cgatcgctct cagcgacatc tttcgcctga cggaacacat cactgtgcgg
    31741 cagaccaagt gcttcacgcg ctttggtggt caggccacgc ccgataatca gcggaatacg
    31801 gccaccagca cgcacttcat caatcagcac gtcggttttc agttcgaagg tcgccagcag
    31861 ttcgccggtt tcgtggttac gcacttcacc tttgtacggg taaacgtcaa tcacgtcgcc
    31921 catgttcagg ttagagacgt cgacttcgat tggcagtgca cccgcgtctt ccatcgtgtt
    31981 aaagaagatg ggtgcaattt taccgccgag gcacaaacca ccgccgcgtt tgttcggcac
    32041 atgtggaata tcatcgccca taaaccacag aacggagtta gtggcggatt tacgcgaaga
    32101 acccgtaccc acaacgtcac cgacgtacgc cagcgggaaa cctttctgtt gcagagcttc
    32161 gatttgcttg atcggaccaa caacaccagg ctggtctggc tcaatacctt cacgggcgtt
    32221 tttcagcatc gccagcgcgt gcagtgggat atccgggcgt gaccacgcat ccggtgccgg
    32281 agaaaggtca tcggtgttag tttcgccagt gactttgaag acggtaacgg tcagtttttc
    32341 agccagcgcc gggcgattca ggaaccattc ggcatccgcc caggactgca taacctgctt
    32401 cgcatattcg ttgcctgctt tcgctttctc ttctacgtca tagaagttat cgaacatcag
    32461 cagcgtgtga gaaagtgctt tggcagcaat aggtgccagt ttggcatcat ccagcgcgtc
    32521 gatcagcgga tgaatgttgt aaccaccctg catggtgccc agcagttcga tggctttttc
    32581 cggagtcagc agaggggatt tggcttcgcc tttcgcgata gcagccagga agcctgcttt
    32641 gacataggcg gcttcatcga cgcctggggg aacacggttg gttaacagat ctaacaggaa
    32701 ttcttcttcg cccgcgggcg ggtttttcag cagctctaca agtgcggcca tttggtttgc
    32761 atccaggggg ttgggcgcaa tcccctcagc ggcacgctca gctacgtgct tacggtattc
    32821 ttctagcacg acggttctcc tcgctctcat tgtcatagtg cggcaggcga ttctcttcac
    32881 gctcctgtga gacagcagtt tgtagggtaa atgcccagta ccgcaacggg cagaatagca
    32941 ggattttgac gatgtgttaa tctgtttaca aaaaagcaac ataaaaaagt ggctgaatcg
    33001 ttaaggatgg tctagagatt gtttccctcc gtaaaaactt cgttaattcg catggtgata
    33061 atcacctttc tcaacgaatc ccaagattag tcaaaattta aactaccgcc tctttatact
    33121 cggattcaca gcacctgcgg gtggcagttc gccgaccatt gcgatttcct tgagatccga
    33181 attatgaaaa tgactttgcc gtttaaaccc catgtgctgg cgctaatttg cagtgccggg
    33241 ctttgtgccg cctctaccgg gctatatata aaaagccgca cagtggaagc gcctgtggaa
    33301 ccgcaatcga cacaacagac tgcgcctgac atcaccgcag ttacgcttcc tgcaacggtt
    33361 tccgcaccgc ccgtaacgcc cgccgtcgtc aaatccgcat tcagcactgc acaaatcgat
    33421 caatgggttg cgcctgtcgc gctgtacccc gattctctgc tttcacaagt gttaatggca
    33481 tcaacctatc cggcaaacgt tgctcaagca gtacaatggt cgcacgataa tccacttaaa
    33541 caaggcgatg ctgctattca ggcggtatct gaccagccgt gggacgccag cgttaaatca
    33601 ctggtggcct ttccacaatt gatggcattg atgggcgaaa acccgcaatg ggtgcaaaac
    33661 ctgggcgatg cttttctggc gcagccgcag gacgtgatgg actcggtaca gcgattgcgg
    33721 caactggcgc aacaaaccgg ctcgctgaag tcatcaaccg aacagaaagt tattaccaca
    33781 acgaagaaag ctgtaccggt aacacagaca gtcacggctc ccgtcatacc atccaatacc
    33841 gtttcaactg ccagccccgt cattacagag cctgcaacaa ccgtcatttc cattgagccc
    33901 gccaatcctg atgtggtcta tattcccaac tacaacccaa ccgtggttta cgggaactgg
    33961 gccaatactg cgtatccgcc ggtttatctg ccaccaccag ccggagaacc gtttattgac
    34021 agctttgtgc gcggattcgg ctatagcatg ggtgttgcta ccacgtacgc actattcagc
    34081 agcatcgact gggacgacga cgatcatgac catcatcatc atgacgatga tgattatcat
    34141 caccacgatg gcggtcatcg tgacggtaat ggctggcaac acaacggcga caacatcaat
    34201 atcgacgtca acaatttcaa ccgtatcacc ggtgagcatc ttactgataa gaatatggca
    34261 tggcggcaca atccaaacta ccgtaatggt gtgccctatc atgatcagga tatggcaaag
    34321 cggtttcatc aaaccgatgt caacggcgga atgagcgcca cgcagctacc tgctccaaca
    34381 cgcgacagcc agcgtcaggc ggcagcaagt cagtttcagc aacgaacaca cgccgccccc
    34441 gtcattacac gagataccca acgtcaggca gcggcacagc ggtttaatga agctgaacac
    34501 tatgggagct atgacgactt ccgcgacttc agccgtcgcc aacccctgac ccagcaacaa
    34561 aaggacgccg ctcgtcagcg ttatcagtcc gcctcgcctg agcagcgcca ggcggttcgc
    34621 gagaaaatgc agactaaccc acagaaccag cagcgaagag aggcagcgcg tgagcgtatt
    34681 cagtctgcaa ctcccgaaca gcgccaggta tttaaggaaa aagtacagca gcgcccactg
    34741 aaccaacagc aacgtgataa cgcccgccag cgtatacaat cggcatcacc tgaacaacgt
    34801 caggtttttc gggaaaaagt tcaggagagc cgcccacaac gtctaaacga cagtaaccat
    34861 actgtcaggc tgaataacga gcaacggtcg gcagtacgcg aacgtctctc tgagcgcgga
    34921 gcaaggcgac aggaaaggta aattgcagac gtaaaaaaag cggcgtggtt agccgctttt
    34981 ttaattgccg gatgttccgg caaacgaaca attacttctt cttcgctttc gggttcggca
    35041 ggtcggtaat gctaccttcg aacacttctg ccgccaggcc cacagactcg tgcagagtcg
    35101 ggtgcgcgtg gatggtcagt gcgatgtctt cagcatcaca acccatttcg attgccaggc
    35161 cgatttcacc cagcagctcg ccgccgttgg taccgacaat cgcaccaccg atcacacggt
    35221 gagattcttt gtcgaaaatc agcttggtca taccgtctgc gcagtcggaa gcgatagcac
    35281 gaccagaagc agcccacggg aaggtggcgg tttcatagct gatgcctttc tctttcgctt
    35341 ctttctcagt cagacctacc catgcaactt ctggttcggt ataggcgatg gacgggataa
    35401 ctttcggatc gaagtagtgt ttcttaccgg cgataacttc agcggcaacg tgaccttcgt
    35461 gaacaccttt gtgtgccagc atcggctgac cgacgatatc gccgatagca aagatgtgcg
    35521 gtacgttggt acgcagctgt ttgtcaacgc ggatgaaacc acggtcgtcc acttccacgc
    35581 cagctttgcc tgcgtcgagg tttttaccgt tcggcacacg accaatcgct accagcacgg
    35641 cgtcgtaacg ctgcggttca gcgggtgctt ttttgccttc catcgtcaca taaataccgt
    35701 cttctttcgc ttcaacggcg gtaactttgg tttccagcat caggttgaat ttcttgctga
    35761 tacgcttggt gaagacttta acgatgtctt tgtcagctgc cgggataacc tggtcgaaca
    35821 tttcaaccac gtcaatctgt gaacccagcg cgtggtatac ggtgcccatt tccagaccga
    35881 tgataccgcc acccattacc agcaggcgtt ctggtacttc tttcagttcc agcgcgtcag
    35941 tggagtccca gatacgcgga tcttcatgcg gaataaacgg cagttggatc gggcgagaac
    36001 ccgctgcaat gatcgcgttg tcgaagttga tcacggtttt accgttctca ccttcaactt
    36061 ccagggtgtt agccccggta aatttaccca gaccgttgac cactttgact ttgcggcctt
    36121 tcgccatacc agccagacca ccggtcagct gattgatcac tttctctttc caggtacgaa
    36181 tcttgtcaat atcggttttc ggttcgccga agacgatacc gtgttcagcc agcgctttgg
    36241 cttcttcgat aacttttgct acgtgcagca gtgctttaga agggatacag ccgacgttca
    36301 ggcaaacacc gccaagggtg ttgtaacgtt ctacgattac ggtttccaga cctaaatcag
    36361 cgcaacggaa ggcagcggag taacctgcgg ggcctgcccc aagtaccacg acctgagttt
    36421 tgatttcagt actcatcatg acctctatat atttatctcc ggcggtcata cccgtcgtct
    36481 ttcaggccgc agcggcgtta gcttcacgcc ctcaccccag tcacttactt atgtaagctc
    36541 ctggggattc tggcgcttgc taccttgctg cacccagaaa tccatagggt atgtgaagta
    36601 ataccctaac caccaccggg tcgttctatc cgtcggtatt ttacaaaatt gttaacaatt
    36661 tttaaacaac aaacggcaac cgatttgtct attcgctaat aacctcaata cattcatgag
    36721 attaccagaa aaaagccggc cgttgggccg gctcttttac ttacatcacc agacggcgaa
    36781 tgtcagacag cgtgttgtta atgatggtaa tgaaacgggc accatcagca ccgtcgatca
    36841 cgcggtggtc gaaggagaga gaaatcggca gcatcagacg cggcacgaac tctttaccat
    36901 tccacaccgg ctccatcgcg gacttggaaa cgccgaggat agccacttcc ggcgcgttca
    36961 caatcggcgc gaagtgggta gtacccaggc cgccgatgct ggagatggtg aagcaaccgc
    37021 cctgcatttc gcccgcagtc agcttaccgt cacgcgcttt cttagaaata gtcatcagct
    37081 cgcgagacag ctcgatgatg cctttcttgt tgacgtcttt gaataccgga acaaccagac
    37141 cgttcggggt atccaccgcc acaccgatgt tgatgtattt cttcagggtc agacgctgac
    37201 cgtcttccga caacgaactg ttgaagcgag gcatctgctc aagagctgca gcaacggctt
    37261 tcatgatgaa gacaaccggg gtgatcttca catccagctt acgcttcgcc gcttcttcgt
    37321 tctgctgttt acggaacgct tccagctcgg tgatatcggt tttgtcgaag tgagtaacat
    37381 gcgggatcat cacccagtta cggctcaggt tcgcaccaga aattttctgg atacggccca
    37441 gttccacttc ttcgatttca ccaaacttgc tgaagtccac cttcggccac ggcagcatgc
    37501 ctgggatacc gccgccagtc gccgccggag ctgcttctgc acgtttgata gcttctttca
    37561 cgtaagcctg aacgtcttcg cgcaggatac gacctttacg gccagtgccc ttcactttcg
    37621 ccaggttaac gccaaactcg cgtgccagac ggcggatcag cggagtcgcg tgaacgtaag
    37681 cgtcgttttc agcaaattca gatttgcctt cggctttcgc agccggagct gctgccggag
    37741 cttcagcttt tgctgccgga gccggcgctg ccgcttcctg tttcgccgga gccgccgcag
    37801 gcgctgcgcc ttcaacttcg aagatcataa tcagcgagcc agttttcact ttatcgccaa
    37861 cgttgacttt cagttccttc acgacgcctg caaacggagc cggaacttcc atagaagctt
    37921 tgtcgccttc tacggtaatc agtgactgtt cagcggcaac tttgtcgccc actttcacca
    37981 tcacttcggt cacttcaact tcgtcaccgc cgatatccgg aacgttaact tctttcacgc
    38041 cagccgctgg tgcaggggcc gctgccggag ccgcttcctg tttagccgcc ggagctgccg
    38101 cgcctgcttc acccgcgact tcgaagacca taatcagcga gccggtagac actttgtcac
    38161 ccacgttcac tttgatctct ttcacggtgc cagcaaacgg agccggaact tccatagaag
    38221 ctttatcgcc ttctacggtg atcagcgact gttcagcttc aactttatcg ccaactttca
    38281 ccaggatttc ggtcacttca acttcgtcgc tgccgatatc cggaacgtta acgtcttttg
    38341 ccgccgcagc cgcgggtgct gctgccggag ctgcttcttt cttctcttct gcctgagcag
    38401 gtgcagcgtc tgctgcaccg tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg
    38461 ttttatcgcc aacagagact ttgatctctt taacgatacc cgcctgcgga gacggaactt
    38521 ccatagaggc tttgtcgcct tctacggtga tcagcgactg ttcggcttca actttgtcgc
    38581 ccactttgac caggatctcg gtgatttcaa cttcatcagc cccgatgtcc ggtactttga
    38641 tttcgatagc cattattctt ttacctctta cgccagacgc gggttaactt tatctgcatc
    38701 gatgttgaat ttggcgattg cgtcagcaac cactttctta tcgatttcgc cacgtttagc
    38761 cagttcgccc agcgccgcaa ccacgacata agaagcatca acttcgaagt ggtgacgcag
    38821 gttctcacgg ctgtcggaac gaccgaagcc atcagtaccc agtacgcggt agtcgtcagc
    38881 cggtacgtaa gtacggacct gctcagcgaa cagtttcata tagtcggtag atgccactgc
    38941 cggagcgtcg ttcatcacct gagcgatata cggaacgcgc ggagtttcca gcggatgaag
    39001 catgttccag cgttcacaat cctgaccatc acgcgccagc tcggtgaagg aggtcacgct
    39061 ataaacgtca gaacctacgc cgtaatcttt cgccaggatc tctgccgctt cacggacgtg
    39121 acgcaggata gaaccggagc ccagcagctg aactttacct ttgctacctt caatggtttc
    39181 gagtttgtag atacctttac ggataccttc ctcagcacct tccggcattg ccggcatgtg
    39241 gtagttttcg ttcagcgtag tgatgtagta gtaaacgttc tcttgttttt caccgtacat
    39301 acgctccaga ccgtcatgca tgatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga
    39361 gatacagttc gggatagtca gcgactgaat gtggctgtga ccatcttcgt gctgcagacc
    39421 ttcaccgttc agggtggtac gaccggaagt accgccgatc aggaagccac gcgcttgctg
    39481 gtcgccagcc gcccagcaca gatcgccgat acgctggaag ccgaacatcg agtaatagat
    39541 gtagaacggg atcatcggca gattgttggt gctgtaagag gtcgccgctg ccagccagga
    39601 acaacctgcg cccagctcgt tgatcccttc ctgcagaatc tgacctttct cgtcttcttt
    39661 atagtaagca acctgctcgc ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat
    39721 accaatctga cggaacagac cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg
    39781 taccagacga tctttgatcg acttgttctt cagcattacg ttcagggcac gaacgaaagc
    39841 gatagtggta gagatctctt tgctctgctc ttccagcagc gcgccgaagt cttgcaggct
    39901 cggcagctca agcttctcgg tgaagttcgg ctgacggctt ggcagataac cgtgcagttt
    39961 ctgacgctgt gcatgcagat aggtatgctc ttcagaacct tccgggaagg tgatgtacgg
    40021 cagtttttcg atatctgcat cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac
    40081 gccgtccatg ttcattttct taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc
    40141 catgccgtaa cctttaatgg tatgagcaag gattactgtc gctttgcctt tggtttcctg
    40201 cgctttcttg aatgcagcgt agattttctt cggatcgtga ccaccacggt tcagtgccca
    40261 gatctgctcg tcagtccagt ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa
    40321 gtgttcacga acgtacgcac catctttcga tttgaaggtc tggtagtcgc cgtcaacggt
    40381 ttcgttcatc agctggatca gtttaccgct ggtatcttta cgcagcagtt catcccaacg
    40441 gctaccccac atcactttga tcacgttcca gccagcacct tcgaagatgc cttccagttc
    40501 gttgatgatc ttgccgttac cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat
    40561 aacgaagacc aggttatcca gtttttcacg ggtagcgatg gtgatcgcac ctttggattc
    40621 cggttcgtcc atttcaccgt cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt
    40681 caggccacgg tgttccagat atttcaggaa tttagcctgg taaatagcac caatcggccc
    40741 cagacccata gatacggtcg ggaactgcca gaattccggc atcagtttcg ggtgcggata
    40801 ggaagagagg ccattgccgt gaacttcctg acggaagtta tccagctgct cctgagtcag
    40861 acgaccttcc aggaaagcac gagcgtacac gcccggggag atgtggccct ggaagtaaac
    40921 caggtcgccg ccatcctgct cgttgcgtgc acggaagaag tggttaaagc acacatcata
    40981 aatggttgcg gaagactgga aggacgccat atggccgccc agttcgaggt cttttttcga
    41041 cgcacgcagc accgtcatga tggcgttcca gcggatagct gaacgaatac ggcgttccag
    41101 ttccagatta cccggatact ccggttgttc ttcaacgggg atggtgttga tgtagttgct
    41161 gatacctgtg cctgcggcta cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc
    41221 gatcagatac tgagcacgct caacaccttc ttcacggatg accgattcga tcgcctggag
    41281 ccagtcgcga gtttcgatcg gatccacgtc atttgggaaa cgttctgaca tgggttattc
    41341 cttatctatc taataacgtt gagttttctg gaacctgtcc cattgaactc tcgccggaaa
    41401 gctcaataag acaggttcta cgtttagttg ccgcgctttt atatgcgctt gatttacaac
    41461 atcttctgga taatttttac cagaaaaatc actaattctt tcgttgctcc agacgacgca
    41521 gagaacgctc acggcggctc tcttcacgac ttctgtcgag caaaatttct tcgataaagg
    41581 ccagatggcg atgcgatgct tcgcgcgctt cttccggctt accggccata atcgcttcaa
    41641 atatgcgggt gcggtgacta ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt
    41701 cgaagttctg gcggacattc tgggccaaca tcggctccat acaccttagc agatgaagca
    41761 gaaccacatt gtgggccgct tcggtgacgg caatctgata ctggagtacg gcgtttgatt
    41821 ccgcgtccag atcgccagac tgctgcgcca gctctatggc gtggtggagt tcacggatgc
    41881 gttccttgtc ttcatcggta ctacgcagcg cggcgtaata agcggcgata ccttccaggg
    41941 cgtgtcgtgt ttcgagcaag tcatactgtg actcaggatg gtcggagagc agctccacca
    42001 gcggatcgct gaagctttgc cataggctgc tctggacaaa agtgccgcca ccctggcgac
    42061 gaagcaacaa gcccttcgct tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga
    42121 cgtcaaactg ttttgccagt tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc
    42181 cttcgaggat caaaaactcc agttgctgct caatcacatc ggagagtttt ggttggcgga
    42241 ttttgctgta ggccatgagt tcctgtctta agccacttgc cgaagtcaat tggtcttacc
    42301 aatttcatgt ctgtgacgct aaagtaacaa agtattcacc ttatgtccat acaggttttg
    42361 attgaaatca tgaaactgtg cacattttaa caacttgaca tatataacgt ttcaaagttg
    42421 taactatgca caaatgtaga ctttacgtag gaaaggagtt ttttaacgat tattaaa
//
LOCUS       DAZGVQ010000022        41711 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000022 DAZGVQ010000000
VERSION     DAZGVQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.022"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            51..287
                     /gene="lpp"
                     /locus_tag="ACHLAG_001168"
     CDS             51..287
                     /gene="lpp"
                     /locus_tag="ACHLAG_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFP0654436.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(351..1355)
                     /gene="ldtE"
                     /locus_tag="ACHLAG_001169"
     CDS             complement(351..1355)
                     /gene="ldtE"
                     /locus_tag="ACHLAG_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFP0654437.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(1504..1920)
                     /gene="sufE"
                     /locus_tag="ACHLAG_001170"
     CDS             complement(1504..1920)
                     /gene="sufE"
                     /locus_tag="ACHLAG_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFP0654438.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(1933..3153)
                     /gene="sufS"
                     /locus_tag="ACHLAG_001171"
     CDS             complement(1933..3153)
                     /gene="sufS"
                     /locus_tag="ACHLAG_001171"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFP0654439.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDSEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPRNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(3150..4421)
                     /gene="sufD"
                     /locus_tag="ACHLAG_001172"
     CDS             complement(3150..4421)
                     /gene="sufD"
                     /locus_tag="ACHLAG_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFP0654440.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(4396..5142)
                     /gene="sufC"
                     /locus_tag="ACHLAG_001173"
     CDS             complement(4396..5142)
                     /gene="sufC"
                     /locus_tag="ACHLAG_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFP0654441.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMVFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(5152..6639)
                     /gene="sufB"
                     /locus_tag="ACHLAG_001174"
     CDS             complement(5152..6639)
                     /gene="sufB"
                     /locus_tag="ACHLAG_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFP0654442.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(6648..7016)
                     /gene="sufA"
                     /locus_tag="ACHLAG_001175"
     CDS             complement(6648..7016)
                     /gene="sufA"
                     /locus_tag="ACHLAG_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFP0654443.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(7564..7752)
                     /gene="ydiH"
                     /locus_tag="ACHLAG_001176"
     CDS             complement(7564..7752)
                     /gene="ydiH"
                     /locus_tag="ACHLAG_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFP0654444.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            complement(7852..8262)
                     /gene="menI"
                     /locus_tag="ACHLAG_001177"
     CDS             complement(7852..8262)
                     /gene="menI"
                     /locus_tag="ACHLAG_001177"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFP0654445.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(8259..11315)
                     /gene="ydiJ"
                     /locus_tag="ACHLAG_001178"
     CDS             complement(8259..11315)
                     /gene="ydiJ"
                     /locus_tag="ACHLAG_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFP0654446.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            11704..12816
                     /gene="ydiK"
                     /locus_tag="ACHLAG_001179"
     CDS             11704..12816
                     /gene="ydiK"
                     /locus_tag="ACHLAG_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFP0654447.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            13002..13109
                     /gene="rprA"
                     /locus_tag="ACHLAG_001180"
     ncRNA           13002..13109
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHLAG_001180"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            13245..13601
                     /gene="ydiL"
                     /locus_tag="ACHLAG_001181"
     CDS             13245..13601
                     /gene="ydiL"
                     /locus_tag="ACHLAG_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFP0654448.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            13701..14915
                     /gene="ydiM"
                     /locus_tag="ACHLAG_001182"
     CDS             13701..14915
                     /gene="ydiM"
                     /locus_tag="ACHLAG_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0654449.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMLINALFLYRCTFPPHPGRRLPVIKKTTRSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLSQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            15142..16407
                     /gene="ydiN"
                     /locus_tag="ACHLAG_001183"
     CDS             15142..16407
                     /gene="ydiN"
                     /locus_tag="ACHLAG_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0654450.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            16419..17285
                     /gene="ydiB"
                     /locus_tag="ACHLAG_001184"
     CDS             16419..17285
                     /gene="ydiB"
                     /locus_tag="ACHLAG_001184"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFP0654451.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            17316..18074
                     /gene="aroD"
                     /locus_tag="ACHLAG_001185"
     CDS             17316..18074
                     /gene="aroD"
                     /locus_tag="ACHLAG_001185"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFP0654452.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDVQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            18218..19813
                     /gene="ydiF"
                     /locus_tag="ACHLAG_001186"
     CDS             18218..19813
                     /gene="ydiF"
                     /locus_tag="ACHLAG_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFP0654453.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERELDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            19827..20978
                     /gene="ydiO"
                     /locus_tag="ACHLAG_001187"
     CDS             19827..20978
                     /gene="ydiO"
                     /locus_tag="ACHLAG_001187"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFP0654454.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(21021..21932)
                     /gene="ydiP"
                     /locus_tag="ACHLAG_001188"
     CDS             complement(21021..21932)
                     /gene="ydiP"
                     /locus_tag="ACHLAG_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0654455.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAISDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            22248..23012
                     /gene="ydiQ"
                     /locus_tag="ACHLAG_001189"
     CDS             22248..23012
                     /gene="ydiQ"
                     /locus_tag="ACHLAG_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFP0654456.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQAGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            23032..23970
                     /gene="ydiR"
                     /locus_tag="ACHLAG_001190"
     CDS             23032..23970
                     /gene="ydiR"
                     /locus_tag="ACHLAG_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFP0654457.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            24026..25315
                     /gene="ydiS"
                     /locus_tag="ACHLAG_001191"
     CDS             24026..25315
                     /gene="ydiS"
                     /locus_tag="ACHLAG_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP0654458.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SGHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            25312..25605
                     /gene="ydiT"
                     /locus_tag="ACHLAG_001192"
     CDS             25312..25605
                     /gene="ydiT"
                     /locus_tag="ACHLAG_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP0654459.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            25662..27308
                     /gene="fadK"
                     /locus_tag="ACHLAG_001193"
     CDS             25662..27308
                     /gene="fadK"
                     /locus_tag="ACHLAG_001193"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFP0654460.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYNYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(27365..29743)
                     /gene="ppsA"
                     /locus_tag="ACHLAG_001194"
     CDS             complement(27365..29743)
                     /gene="ppsA"
                     /locus_tag="ACHLAG_001194"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFP0654461.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            30076..30909
                     /gene="ppsR"
                     /locus_tag="ACHLAG_001195"
     CDS             30076..30909
                     /gene="ppsR"
                     /locus_tag="ACHLAG_001195"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFP0654462.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            31066..32112
                     /gene="aroH"
                     /locus_tag="ACHLAG_001196"
     CDS             31066..32112
                     /gene="aroH"
                     /locus_tag="ACHLAG_001196"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFP0654463.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVAIHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            32244..32435
                     /gene="hemP"
                     /locus_tag="ACHLAG_001197"
     CDS             32244..32435
                     /gene="hemP"
                     /locus_tag="ACHLAG_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFP0654464.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(32439..33875)
                     /gene="selO"
                     /locus_tag="ACHLAG_001198"
     CDS             complement(32439..33875)
                     /gene="selO"
                     /locus_tag="ACHLAG_001198"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFP0654465.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDIERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(33938..34651)
                     /gene="ydiV"
                     /locus_tag="ACHLAG_001199"
     CDS             complement(33938..34651)
                     /gene="ydiV"
                     /locus_tag="ACHLAG_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFP0654466.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            complement(34898..35362)
                     /gene="nlpC"
                     /locus_tag="ACHLAG_001200"
     CDS             complement(34898..35362)
                     /gene="nlpC"
                     /locus_tag="ACHLAG_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP0654467.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(35440..36189)
                     /gene="btuD"
                     /locus_tag="ACHLAG_001201"
     CDS             complement(35440..36189)
                     /gene="btuD"
                     /locus_tag="ACHLAG_001201"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFP0654468.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            complement(36189..36740)
                     /gene="btuE"
                     /locus_tag="ACHLAG_001202"
     CDS             complement(36189..36740)
                     /gene="btuE"
                     /locus_tag="ACHLAG_001202"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFP0654469.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(36803..37783)
                     /gene="btuC"
                     /locus_tag="ACHLAG_001203"
     CDS             complement(36803..37783)
                     /gene="btuC"
                     /locus_tag="ACHLAG_001203"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFP0654470.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADVVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            complement(37994..38290)
                     /locus_tag="ACHLAG_001204"
     CDS             complement(37994..38290)
                     /locus_tag="ACHLAG_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654471.1"
                     /translation="MPDKLEKRVERLEADVADIKSNIAILTTRSESFATKSDVLEIRE
                     GLRLEMADLRQSLKVEMADHRTEIQKAFTSQTWQLASIIVSAMAALVAIMVAIK"
     gene            complement(38395..38757)
                     /locus_tag="ACHLAG_001205"
     CDS             complement(38395..38757)
                     /locus_tag="ACHLAG_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654472.1"
                     /translation="MTKPLDELERMATPDNFARFLNDSSANEKCLSCGSTDMYMHLSD
                     VVEVGPEPRSAEECNLGTRIQLDYTGPCTMYPGYNGHDREYIHNYEFKVMCSRCGFIH
                     RYSAQAFMSWLNHRGGEK"
     gene            complement(38760..39698)
                     /locus_tag="ACHLAG_001206"
     CDS             complement(38760..39698)
                     /locus_tag="ACHLAG_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0654473.1"
                     /translation="MRPDGRKGKRIRKKFKTKSDAVLYERWVLSQQHSNEWKSAPTDR
                     RPLSELIDLWWKYHGQLMRSGRNTHLKLLRLCELMNDPGVHKVNATTLTELRVARAEE
                     GIQPSTINREVGALSSMFSALISSCHFLNDNPVQGLKGMKVVEREMGYLSKPECLRLL
                     DALAEHPDERLAVEILLSTGARWGEVVALEQRRVQHCRITFSRTKNSKNRTVPISESL
                     FEKIKNRGGKRVFPVLDYPLVRDAIKVVAPDVPDGQAVHALRHTFASHFMMNGGNILT
                     LQKILGHARIQTTMIYAHLAPDYLQDAVRFNPLGED"
     gene            complement(39787..40095)
                     /locus_tag="ACHLAG_001207"
     CDS             complement(39787..40095)
                     /locus_tag="ACHLAG_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0654474.1"
                     /translation="MLIGEKIRVIREFEDLTREEFCGLIDVPIGTLRRYETGRIENVG
                     SDVLVKIVNHPRFFKYMNWLLTGKTNEAAGQISPALSPDGPESTSSSLKPRKTGTQHG
                     "
     gene            40193..40471
                     /locus_tag="ACHLAG_001208"
     CDS             40193..40471
                     /locus_tag="ACHLAG_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0654475.1"
                     /translation="MAKIQNERGQVEIERLMALYPYRDERGLPILEESLKDYAKRTNQ
                     AFNTVKRQADRAQIPINQDEKYGKRTVNLYAIFLKTIRNAEKYVQMTK"
     gene            40490..40813
                     /locus_tag="ACHLAG_001209"
     CDS             40490..40813
                     /locus_tag="ACHLAG_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFP0654476.1"
                     /translation="MKNQRNAGLHRSKERLVHRQDFRNTNTEIHATPDGHPVKQTGKH
                     TWTIGDTGIVIHKSFRSPVTGRFNFTLTRGDDYFGQDFTFFEALRTADRLINGLCFQY
                     PGSKH"
     gene            40832..41074
                     /locus_tag="ACHLAG_001210"
     CDS             40832..41074
                     /locus_tag="ACHLAG_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFP0654477.1"
                     /translation="MIEAYKILINNALQRYHFELANRGVTKDESLRNSLRGAIMGLYN
                     CALGSDDREALEELKSIMTELFEGEIVEPYFTGVAA"
     gene            41071..41196
                     /locus_tag="ACHLAG_001211"
     CDS             41071..41196
                     /locus_tag="ACHLAG_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654478.1"
                     /translation="MSIFLSWVILIISVLMAIGIMRIIHTLKDVELFFTGDKRRK"
     gene            41414..41647
                     /locus_tag="ACHLAG_001212"
     CDS             41414..41647
                     /locus_tag="ACHLAG_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654479.1"
                     /translation="MRTAESPSREITARPEQPHAHISMTARKAERAHICELLRDLVNT
                     AKEKHTSPLETCSAIERLIATLTNNAEQKECTQ"
BASE COUNT        10489 a        10676 c        10103 g        10443 t
ORIGIN      
        1 aatacttgta acgctacatg gagattaact caatctagag ggtattaata atgaaagcta
       61 ctaaactggt actgggcgcg gtaatcctgg gttctactct gctggcaggt tgctccagca
      121 acgctaaaat cgatcagctg tcttctgacg ttcagactct gaacgctaaa gttgaccagc
      181 tgagcaacga cgtgaacgca atgcgttccg acgttcaggc tgctaaagat gacgcagctc
      241 gtgctaacca gcgtctggac aacatggcta ctaaataccg caagtaatag tacctgtgaa
      301 gtgaaaaatg gcgcacattg tgcgccattt tttttgtctc ccgtttaccg ctactgcgtc
      361 gcgcgtaaca tattcccttg ctctggttca ccattctgcg ctgactctac tgaaggcgca
      421 ttgctggctg cgggagttgc tccactgctc accgaaaccg gataccctgc ccgacgatac
      481 aacgctttat cgactaactt ctgatctaca gccttattgt ctttaaattg cgtaaagcct
      541 gctggcagtg tgtatggcat tgtctgaacg ttttgctgtt cttctgctga tagtggtcga
      601 tgtacttcaa cataacgcat cccgttaggt tccacggaat atttcaccgg ttcgttgatc
      661 actttcaccg gcgttcccgt ccgcacgctg gagaacaagg ctttaatatc cggtgcattc
      721 atgcgaatac accctgaact gacgcgcaaa ccgacgctgt ccggcgcact ggtaccatga
      781 atgaggtatt cgccattacc atgcgcgagg cgcagtgcgt aacgtcctag cgggttattt
      841 ggtccggcag gaacgactgg cggtaattta atgccacgct ccagcgaacg ttgacgaatg
      901 cctgccgtag gcgtccaggt tgggttaggg attttctgcc caacacgcgt ttccatcacc
      961 ggcgtttcca gcccctgcaa tccaatacct attggataaa cctgcacaat attttctccc
     1021 ggcggataat aataaaggcg cagctctgca aggttaacga taatcccctg acgcggtgca
     1081 tcaggtaata acagttgtga aggaatagtt atcgtcgtac caggttttgg caccggggcg
     1141 atagtgttat tggcttcaag gatcaacatt gccgcagtat caaaacgtcg ggcaatagcc
     1201 tgaaggtttt tatccccttc ttgcaccgta tacgtttgat tttgcccaac cagtcggctt
     1261 ccggttggtg gtagcggata atcaaccgcc caggcagcct ggatggcgct aaaagcgccg
     1321 ataagcgtga gtgtaagcaa agacgcgcgt ttcattgtaa acctcctgta tttgccggag
     1381 actcacgctg aaacgtcgga tggcgcttat gttcacctga aaccaaaaca ctcctgtgca
     1441 ggtcagtgta aacattgacc atccggcaat gtgagccaac cggatgaaag ctgtcccttt
     1501 agtttagcta agtgcagcgg ctttggcgcg aattgcgcga atcatcgctt ccagaccttg
     1561 tgaacgagat ggggtgagat gttgggtgag cgccattttt tcaaaccacg gacgcacatc
     1621 gaaattgaca atatcctgcg gcgtcatctg atcgtagaga ataaagacga ccgcaataag
     1681 ccctttcaca atcgccgcat cgctgtcgcc ctgcaattca ataattccct gcgcattctg
     1741 gcgcatgaca atccacacct gactctgaca gccctgaatg ctattttgtg ggcttcggtc
     1801 ttcggcgcgt aattctggca gacgctggcc cagctcaata atgtagagat atttctcttc
     1861 ccagttggcg cagcgtaaaa aattacgcag caatttttct ttatccggca ataaagccat
     1921 agtgcctccc tgttatccca gcagacggtg aatacgttgc agtccggtca ccagacgatc
     1981 cacttcttca tgggtgttat acatggccag cgacgcccga cacatagcag ggacgttgta
     2041 ataggccatt aatggcatag cgcagtgatg tccggtacgc acagcaatgc cgtagttatc
     2101 gagaaaactg ccaacatcat aggcgtggtg tttaccaaga ttaaaagcaa taacgccaag
     2161 cctgttccgc gggccataga gagtgagatc cggtacagat tccagctgtg atagcgcgta
     2221 gtgcatcaga ttctgttcat actcggctat gttattaagc cccagcgccg aaacatactc
     2281 cagcgccgcg ccaagaccaa tgatgccccc ggtattgggt gtaccggctt caaaccgcca
     2341 tggtgctttg gtccaggtag tgccttcact caggctgacg gtggcgatca tagaaccgcc
     2401 cccttcccac ggcggcatct cctgcaacaa ggcttctttc acataaagaa tgccaattcc
     2461 ggtggggcca tacagtttat gcccggagaa tacgtaaaaa tcgcaatcca gcgcctgaac
     2521 gtccaccgga tgatgcatca ccgcctgagc gccatccacc agcacttttg cgccatgctg
     2581 atgcgcaagc gtgatcattt ccgccagggg attttctgtg ccaagcacgt tggagacatg
     2641 agtaattgcc agcaggcgag ttttctcatc gaacagatta ggtagcgtct ccagttgcag
     2701 cgtaccgtcg ggattgagcg ggatcacacg cagctctgcg ccaacgcgtg cgcaaagcat
     2761 ctgccaagga acaatgttag cgtggtgctc catctgactg atgatgatgt tatcgcccgc
     2821 ccgcacgttg ctgttgcccc aactattggc gaccagattg atcccttccg tcgtgccgcg
     2881 gacgaacacc agctcttccg ccgaacgggc attaataaac agcgatgccc gcttgcgcac
     2941 gttctccatt ttctcggtcg cctgggcgct taaggtatga ataccgcgat gcaccgccgc
     3001 gtagccgtga cgataaaact cggcttcgga gtcaatcacc tggcttggtt tctgcgcact
     3061 ggcggcgctg tcgagataag ccagcggcaa accgtttacc tcacgcgaaa gcaccggaaa
     3121 gtcggcccgc actttgtcga cggaaaaagt catcttgcac ctcctggcag tcgttgaccg
     3181 attcgggcca gcacctgctg tttaagcccc tcatcacgca gtgcttccgt cagttcggca
     3241 gcaaaggcgt aaatgatcat ctgctgggca tcctgctgat tgatcccgcg cgagcgcaga
     3301 tagaacatct gttcatcatc aatacgcccc accgtcgcgc cgtggctgca tttcacatca
     3361 tctgcataga tttccagctg cggtttcgta tccacttctg ccagtttgcc catcagcaga
     3421 ttattgttgg tcatctgacc atccgttttg atggcgtgct gcgcgacgtt gatcaaaccg
     3481 ttaaataccg cgcggccttt gtcgctgacg atagttttgt gcaactgtcg gctgttacaa
     3541 aaacctttat tgtgttccag ccaggtacgg gtatcacaca cctcgttttt caccggcatc
     3601 gccaggctat tgatccgcag cgtgctgttt tcgccattga gttgcgtact ggtgttgtgt
     3661 cgtaacactg cgccacccag caggaaacta tggctaaatg cggtggcatc gtcagccagc
     3721 aacaaatcgt tatgggcaaa gtggtgactg accgggtttt caaacgccag cttgatatgc
     3781 tgcaagtggg cgttcgctgc gacattgata gtgaaccgtg cgccggtgaa gtgacgagca
     3841 tcattaaggc tgacaaaatg ttcgatcacc gttgcttcgg caccttccgc cagatccaga
     3901 tgatgtcggt aatgggcagt gttgacctct tcacctgcca cgccctgggt aatatgcatt
     3961 aacagcaatg gctttgccgg ccgttgaccg cgcttcacgg cgatatgcgt cacgctttgt
     4021 gccaggcttt ccgtcaaatg cagaaacact tccgcctgaa tagcgtcggg taccccctga
     4081 cggtcgtcgt taatgctcac ttcatacccg ctgccttcag tcgcatcgct cagtgctgac
     4141 acgtaacgtc catcgacaaa taccagccgc acagcgtcta acgttaacgc taaggcatct
     4201 cgctgctgtg gggatatctc tcccgcaatg ctgacaaact ggctattggt cagcccttcc
     4261 agcggcgtat atttccagtt ttcatgttta cgtgtcggca gtccggtacg cagcaattgt
     4321 tgtaaatgct gctgtgcttg cggtgagcgt tttgtccctt cagcttcaaa caagtgatgc
     4381 cactgttgca gcgcgttact gctgttcggt aagccagcca taaccctgct cctccagttg
     4441 tttgaccaac gtgaaatcgc cggatttcac aattcgtccc tgatatagca catggacgta
     4501 atcaggcttg atgtagtcga gaatgcgttg gtagtgcgta acaatgatga atgagcgctt
     4561 gccatcacgc agcgagttca cgccatcggc gaccactttt aatgcgtcaa tatccagccc
     4621 ggagtccgac tcatcaagaa tgcataactc cggttccagc accgccattt gcaaaatatc
     4681 gttgcgcttt ttctcgccgc cggaaaaacc aacgtttacc gaacgggtta ataaatcttc
     4741 cggcatcttc aggagagcga ttttctcttc catcaaatcc tgaaaatcaa agcggtcgag
     4801 cgtttcctgg ccgcgatagc tgcgcactgc attaagtgcc gtttgcagga aaaactggtt
     4861 actgacgcct ggaatctcca ccggatactg gaagaccata aagatgcctt cgcctgcacg
     4921 atcttccggc gacagtgcaa gcaaatcttt gcctttgaac tcaaccgtgc cgcccgtcac
     4981 ttcataatct tcgcgcccgg caagcgttgc cgataaggta cttttgcccg aaccgtttgg
     5041 ccccataatg gcgtgaactt cgccgggacg aacggcgagg cttaatccac gcaggatagc
     5101 tttatcttcc acgctgacgt gtaaatcttt aatacttaac atgtttattc cttatccgac
     5161 gctgtgttca agactgatgg cgaggagttt ttgtgcttca acggcaaatt ccagcggcag
     5221 ttctgagaac acgtctttgc agaaaccgtt aacaatcatc gagatggcgt cttcttcgct
     5281 gatcccgcgt tgcaggcagt aaaacagttg atcttcacca atacgtgatg tcgttgcctc
     5341 gtgctccagt tgtgcgctgt tattgcgaca ttcgacatac gggaaggtat gcgccccaca
     5401 attagcgcca atcagcattg agtcgcactg agtgaaattg cgcgcattgg ttgccgtcgg
     5461 catgattttc actaagccgc gataactgtt ctgactatgt ccggcagaga tccctttcga
     5521 gataatggtc gatttggtgt ttttaccgat gtggatcatc ttggtgccgg tatccgcttg
     5581 ctgatgaccg ctggtcagcg ccactgagta aaactcacca atggagttat cgccgcgcaa
     5641 aatgcagctg ggatatttcc acgtaatcgc ggacccggtt tctgattgcg tccatgacat
     5701 tttgctgttt tcgccttcgc acaaagcacg cttggtgacg aagttgagaa taccgccggt
     5761 gttgttatcg ccagggaacc agttttgtac cgtggaatat ttcacctcgg cgtttttatg
     5821 gatgatgact tccaccactg ccgcgtgtaa ctgatagctg tcacgcaccg gagcggaaca
     5881 gccttcaatg tagctgacgt agctgtcttc gtcggccacc agaatggtgc gctcaaactg
     5941 cccggttttt tccgcgttaa tgcgaaaata ggtggaaagt tccatcgggc agcgcacgcc
     6001 tttaggcaca taaataaacg taccgtcaga ggctaccgcc gcattaagcg cggcaaagaa
     6061 gttgtcattc cccggcacca cggtgccgag atatttacgc accagttccg ggtgatcgtg
     6121 gatcgcctca ccaaaggaac agaaaataat tccctgctcc gccagttttt cgcgataagt
     6181 agtggcaacc gaaactgagt cgaaaatggc atccaccgcc acctctttgc cttcccgcac
     6241 gggaacgccc aactgctcaa acgccgcctc cacctcttta cttaaaaagg cgttcgcgcc
     6301 agtttgctgc accgcgccag gttcagacgc gcaagtgtcg tcacaattac cgcacgatgg
     6361 tgctgagtag tagctgtaat cctgataatt cagcttgtcg tagtgcgctt tcaaccagtg
     6421 cggttcttcc atctccagcc atgcgcgata ggcgtttaga cgaaacttca gcatccactc
     6481 cggctcatta cgcttcgccg aaattgcgcg caccacctct tcgtttatcc cctttgccag
     6541 ctcatcggtg gctaactggg tgaagaatcc ttctttataa ttcagcgggc cgccggtcca
     6601 ggttttgaca tcgtcagttg cttcagtatt acgagacata gtaccgccta taccccaaag
     6661 ctttcgccac agccacattc attctgggct ttagggttgt gaaatttgaa tatctgatta
     6721 agtccttcac gaacgaaatc gacttccgtg ccatcaataa acggcatcgc ttgcagcggg
     6781 acaaacagct tcgcgccgtc gtgttcaaac agcagatcgt ctttgtccgg ctcgctaaca
     6841 ctgtcgagca catagccaaa gcccgcgcag cccgtttgct tcacgcctaa gcgcacgccg
     6901 accatacccg gctgctttgc caccagctca cggatgtgta tcgccgctgc gggtgtcagc
     6961 gttaagcctt gccaggcgaa atcttgtggg ttaaaggttc ctgaatgcat gtccatcgat
     7021 ttacctcact tcatcgcttt cagcgtataa cagcatgtta gtgataatga ttatcagtac
     7081 aacccagaaa acgcaggggc tttaccgtaa aacatgcttt ttgcctgctt ttaataagca
     7141 tagaccctga tgtgtgggtt aacaggcacg ctaagaatta ggtatctcat tgttagataa
     7201 tggttattat ctaaggagca ttaaggctgt gatgaaaaag aaaaagttgt attgaaaatg
     7261 actatttaag agataggtaa aaaagtgcag cgttcagaaa taagaaaacc cttaagtctg
     7321 tgcaacacag gcttaagggt ttctacccca tccggcgctt atctccggca ctctcagtgg
     7381 cttagctctt gaaggggcga taagaataat ctcataaagc taacccgccg ttttaacaca
     7441 aactgcgatt agtattattt ttgaacaata tcaggcggta gataagcagt attaagaagg
     7501 tcatcgaacc tggacggagg ttaatccagg tcgatttggc gaacttgcgg cattaagtca
     7561 ggatcaatgc acgcccagac gccaggcaaa gtagatttct tcttttaatt cagcagaaga
     7621 gagagtaagc aggtcagcaa attcaagttc tagttgtttc agacgtttga gatattgggc
     7681 aggtgaaaga ttgctctggt cacggcgtaa aaattcaatg gccagctggg tgggatcaag
     7741 ttgagtagac atagcatcct cgcttttaga caagacctgc acagtatacc accgtttact
     7801 gtgcagataa tgaccaaaag caatatgcgt cacacttttc tggtgacaac gtcacaaaat
     7861 ggcggtcgtc aatcgtgacg aacagcacaa acgccctttc tcatcgaaga tttcaatctg
     7921 ccagacctgg tgacgcgaac cgagatgcaa cggtttacat atgccgcgca cccgcccttc
     7981 tcgtgccgag cggacgtgat tagcattgat ttccagacca accacttttt gctcaccttc
     8041 ggtacataaa taaccggcaa cggaaccgat actttcggcc agcaccacgg atgcgcctcc
     8101 atgcagcaac ccgaaaggct gctttgtccg cgagtctact ggcattgtcg cttcaagggt
     8161 gtcatcacca atatgttcaa agcgaatatc cagtaacccc accatgtttc cttcacccat
     8221 ggcattcagt gcttccaggg tgatttttcg tttccatatc atttaataat ctccagtaaa
     8281 gcctgcacag gatggcgtac ccccgtgcct tcaacccgtt tgacctgact acggcaggaa
     8341 tatccggtcg ccagacagcg gttacgcggc agtcgctgca tagcctgatg ccaggataac
     8401 tcatagatcc cgagcgaatt tttatggttt ttcgcttcat gtccgtaagt ccctgccatg
     8461 ccgcagcaac ccacgctgac attttccagt ttcgcgccaa aacgggcaaa tatcgcggcc
     8521 cattgtgctg gcgcacccgg caaggcggta acttcagtac agtgaccaaa gaaataccat
     8581 gattcaccgc tgactgtagc cactggctgt gactcaagtg cgcttgccag ccattcattc
     8641 gccagtaaga cgttaaactc gccacgttcc tcgcccagcg ccagtttata ttcatcgcga
     8701 taacaaagta ccagcgccgg atcgacgccc accattggca tacccagctt cgccatacgg
     8761 ttgaggaaat ccgccgtctt tttcgccgtc ttcgcaaaac gattaagaaa acctttaata
     8821 tgctgggctt tgccatttgg cgaaaatggc agtaacacag gctggaaacc taatttttcg
     8881 accagacgga caaaatccgc caccacttgc gcatcgtaat agctggtaaa ggggtcctgc
     8941 accaccaaca ctgtgcgcgc tttctgctct gcattgagag attcaagctg ttccagcgtc
     9001 atgtttgccg agcgatgccc caccatttgt tgttgtagcg aggggaccga cagcagcggc
     9061 aaatcaacca tgccgatatg tttttccgag agtttgcgca ccagcggctg gttaatgaag
     9121 aagttaaagg ttttcggcgc gcgtgccatc agcggcgcgt agctctcgac cgtagcgacg
     9181 aggtggtcgc gcagcgggcg taaataacgg gtgtgataga gctgcagaaa acgagagcga
     9241 aactccggca catcaatttt gatggggcac tgggtcgaac acgctttaca ggccagacag
     9301 cccgacatcg cctctttgac ttcgtgtgag aagtcatatt cgcctttatt cgcatgccag
     9361 ctattgcgcg tgcgggcaat taacgtccgc aaactgacgc cggattcagg cagttcttgt
     9421 tccagtttga gtggatcaac gccgcgatcc gccaacaaac gcagccattc acgcaccagc
     9481 gttgcgcgcc ctttcggtga atgaatccgg ttctgggtga tcttcatcga cggacacatc
     9541 ggactacggg catcaaagtt gaagcataaa ccgttgccgt tacactccat cgcaccgcgc
     9601 cactgctggc gtaccgcaat ggggatctgc cgatcgaatg taccgcgctt caccgcgtcc
     9661 actttcatca tcggcgcatc gagaccttct ggcgggcaaa tcttccctgg gttgagtcgg
     9721 ttatgcgggt caaatgccgc tttcactttg cgcagttctg caaaaagttc ctcaccgaaa
     9781 aacgccgggc tgtattcagc gcgaaaacct ttgccgtgct cgccccacaa caaaccaccg
     9841 tatttcgcag tcagcgccac tacgtcatca gagatttgct tcatcaaaat ctcttgttga
     9901 ggatcgcaca tatccagcgc tggacggacg tgcaagacac ctgcgtcgac gtgaccgaac
     9961 ataccgtagc ttaagccgtg gctgtcgagc agcgcgcgaa attcagcaat ataatcggcc
    10021 agatgttccg gcggtacgca ggtatcctca gcaaacggaa ttggcttagc ggcacctttg
    10081 gcattgccaa gcagaccaac ggctttttta cgcatcgcat agatacgttc aacacccgcc
    10141 agctcgcggc acacctgcca gccaatcaca cctgcctgtt ggctggcaat cagctcatca
    10201 agccgcacac agagagcatt aactcgctcg tcaatcagcg cctcatcatc accagcaaac
    10261 tccacaatgt tcagcccgag catctctttg tcaggcacat cggtaatcaa ctcgctgacg
    10321 gaatgccaga caatatcttc ccgcgccaga ttcagcactt ttgagtccac cgtctctacc
    10381 gaaagcgccc gcgcctcaac cataaacggc gcgttacgca gcgcggagtc aaaagagtca
    10441 tatttgacgt tcaccagacg gcgcacttta ggcaagggcg taatatccag ccgcgcttcg
    10501 gtaataaagg ccagcgtccc ttctgaaccc gtcagaatgc gcgtcaggtc gaactcggtc
    10561 atctcatcgt taaagacatg acgcagatcg taaccggtaa gaaagcggtt aagtttgggg
    10621 aagttgtcga tgattaactg gcgttgctga cggcaacgtt gataaaccgt gttataaatt
    10681 cgcccgattg tggtattgga tttacccagc gtttccgcca gttcgacggg taaaggttgc
    10741 gtatcgagaa tatcgccccc caacaacacc gcgcgtacgc caagtacgtg atccgacgtt
    10801 ttgccataga ccagcgatcc ctgaccggat gcatcggtat tgatcatccc accgagcgtt
    10861 gcccggttgc tggtcgaaag ttccggcgca aaaaagtagc cgaacggttt caggtactga
    10921 ttgagttgat cttttatcac cccggcctca acgcgcaccc agccctcttc agggttaatt
    10981 tcgatgatgc ggttcatatg gcgggacata tcaacaataa tcccctggtt gagcgcctga
    11041 ccgttagtgc cggtgccgcc gccgcggggg gtaaagatca gcgatgaata gcgttcctgc
    11101 gcggcaagac gggcgatcag cgccacatct gcggttgaac gcggaaatac caccgcatcg
    11161 gggagaagtt ggtaaatact gttgtcggtc gacattgtca gacgatcggc ataacttgtc
    11221 gccgtatcgc cggtaaaacc ttgttgctcc agctcttgca aaaaattaag caccagttga
    11281 acgacgccgg gtgcctggga aatctgtgga atcattatat tgaccctttc ctgcggtctg
    11341 tgatgtaggt cgatacacta ttctttcagg cggctgcaat agcgcactga aaggtgatgt
    11401 ttatttactc tatggatttc gagttgcagg aaggcggcaa gcgagtgaac tccaggagct
    11461 tacaatagta agtgactggg gtgaacgaac gtagccgcag cacatgcaac ttgaaatacg
    11521 acgagtaaat cgtttgcgtg ttgcctgagt tgttgtacca catttttttc taacacgccc
    11581 atcagaatta agggcagaat cggcctgtta aaaaccgctg aaattgctca tcattatgca
    11641 ggtgagtttc gcgtgttcac gtcgcgtcga cgatttgacg cacaaaaaag gtgaaaagta
    11701 gttatggtaa atgttcgtca gcccagggat gtcgcacaaa ttctgctttc ggtgctgttt
    11761 ttagccatca tgattgtggc atgtctgtgg attgttcaac cctttattct cggctttgca
    11821 tgggccggta cggtggttat cgccacctgg ccggtattgt tacgtttgca aaagatcatg
    11881 tttggccgcc gctctctcgc cgttctggtg atgacgctgt tattagtgat ggtgtttatc
    11941 atccctattg ctttgctggt taacagtatc gtcgacggca gcggcccgct aattaaagcc
    12001 atttccagcg gcgacatgac gttacccgat ctggcgtggc ttaataccat tccggtgatt
    12061 ggcgcgaagc tgtatgcagg ctggcacaac ttgctggata tgggggggac ggcgatcatg
    12121 gcgaaagtcc gcccttatat tggcaccacc accacctggt tcgttgggca ggcggcgcat
    12181 atcgggcgct ttatggtgca ttgtgcgctg atgcttctct tcagtgccct gctgtactgg
    12241 cgcggtgaac aggtggcaca aggcattcgc cattttgcaa cccgtctggc aggcgttcgc
    12301 ggagatgccg ccgtgctgct ggcggcacag gctatccgcg cggtggcgct gggtgtggtg
    12361 gtgacggcgt tagtacaggc agtgcttggc ggtatcggcc tcgccgtatc cggcgtacct
    12421 tatgcaacgt tgctaacggt gttaatgatc ctctcctgcc ttgtccagct tggcccgttg
    12481 ccggtactga ttccggcgat tatctggctc tactggaccg gcgataccac ctggggaacg
    12541 gtattgttag tgtggagcgg tgtggttggc acgctggata acgtcatccg cccaatgtta
    12601 attcgcatgg gtgccgattt accgctgatc ctgatcctct ctggcgttat tggtggtttg
    12661 attgctttcg ggatgatcgg tctgtttatt ggtccggttc tgttagccgt ttcctggcgt
    12721 ctgtttgccg cgtgggtgga agaagtcccg ccgccgacgg accaaccgga agaaattctc
    12781 gaagaacttg gcgaaatcga gaaaccgaat aagtaatttc tcatcaggcg gctctgccgc
    12841 ctgattgtta accaccgcta attgatattt gcttgctctt ccccatattt tcctgcttac
    12901 cgccattcag ctggtagtac ctgtcgcaaa ttctttacag tttttaaact aatgagacga
    12961 atctgatcga cgcaaaaagt ccgtatgcct actattagct cacggttata aatcaacata
    13021 ttgatttata agcatggaaa tcccctgagt gaaacaacga attgctgtgt gtagtctttg
    13081 cccatctccc acgatgggct tttttttaac atttttccgc ttcgctacct cgcccctcac
    13141 tctttccaat aatgttgcac aagatactgt gactctcttc aagccgtcag caggctgata
    13201 atgttattta tattttattg tttatttcat gcagccatta cacgatgaac gcttatgaac
    13261 tccaggcact ccgccatatt tttgccatga ctattgatga atgcgcgacc tggattgccc
    13321 agaccggtga cagtgaaagc tggcgtcaat gggaaaatgg caagtgtgcc attcctgatc
    13381 gtgtagtcga gcaactgttg gctatgcgtc agcaaagaaa aaaacatctt catgccatca
    13441 tagagaaaat aaataaccgc atcggcaaca acaccatgcg ttttttcccc gacttaaccg
    13501 catttcaacg agtctaccct gatggcaatt ttattgactg gaagatctat caatcggtag
    13561 ccgctgaatt atatgcacac gatctggagc ggctttgcta atttttccac agaaaggaat
    13621 tgtcgttgtt acaacaataa tgaatggata ctgacacaac atcgctacac tttttaaagc
    13681 acctttgcta agtagaacct atgaaaaatc cctattaccc taccgcactt gggttgtatt
    13741 ttaattacct ggtgcatggt atgggcgtca ttttgatgag cctgaatatg gcctcgctgg
    13801 agacactttg gcagactaat gccgcgggtg tctcgatagt tatctcatcg ctgggcattg
    13861 gtcgattaag tgtcttgctt tttgcaggat tattatccga tcgctttggt cgccgccctt
    13921 ttatcatgct cgggatgtgt tgctatatgg ccttcttttt tggcatcctg cagaccaata
    13981 acatcattat cgcttatgtt tttggctttc tggcaggaat ggcaaacagt tttctcgatg
    14041 caggcactta tcccagtttg atggaagctt ttccacgctc acctgggaca gccaatattt
    14101 taattaaagc atttgtttcc agcggacaat ttttattacc gctaatcatt agcctgttag
    14161 tgtgggctga actgtggttc ggttggtcct ttatgattgc tgcaggcatt atgcttatta
    14221 acgctctgtt tttataccgt tgtacgttcc caccccatcc gggtcgtcgc ttacctgtca
    14281 taaagaaaac cacccgctct acggaacatc gctgttcaat tatcgattta gccagttata
    14341 ccttatatgg ctatatctca atggcaacgt tttatctggt tagccagtgg ctgtcacagt
    14401 acggacaatt tgttgcaggc atgtcataca ctatgtcgat caaactactc agtatctaca
    14461 ccgtgggttc gctgctttgt gtatttatta ccgctccact cattcgaaat accgttcgcc
    14521 caacaacatt actgatgctg tacaccttta tctcatttat tgctctgttt accgtctgcc
    14581 tgcatcccac attttatgtg gtgataatat ttgcttttgt cattggtttt acctctgctg
    14641 gaggtgttgt gcaaattggc ctgacgttaa tggctgaacg tttcccctac gctaaaggta
    14701 aagctacagg gatctattac agtgcgggca gtattgcgac ctttactatt ccgttgatta
    14761 cggctcatct gtcccaaaga agtattgccg atattatgtg gttcgatacc gccatcgctg
    14821 ccatcggctt tttactggca ctgtttatcg gcttacgcag ccgcaaaaaa acgcggcatc
    14881 actcgctaaa ggaaaatgtc gctccgggtg ggtaatgcaa tattcttttc aggtcatgca
    14941 agatcttacg gataaataac tctttctgcg ctaactaagg aaaatcacga tcaaaaacaa
    15001 actatgacat gcaatattcc tggaaacata aactttatgc catgtaccca gggaaaatca
    15061 tcttcagtat agtaattatg taaaccgtcg gagaacaata cgtacggtaa cgaaattatc
    15121 tttcagcaag gagctgtgaa aatgtctcaa aataaggctt tcagcacgcc atttatcctg
    15181 gctgttcttt gtatttactt cagctatttc ctgcacggca ttagtgttat tacgcttgcc
    15241 caaaatatgt catctctggc agaaaagttt tccactgaca acgcgggcat tgcctactta
    15301 atttccggta tcggtttggg gcgattgatc agtattttat tcttcggtgt gatctccgat
    15361 aagtttggtc gtcgggcggt gatattaatg gcagtaataa tgtatctgct attcttcttt
    15421 ggtattcccg cttgcccgaa tttaactctc gcctacggtc tggcagtgtg cgtaggtatc
    15481 gctaactcag cgctggatac gggtggctac cccgcgctca tggaatgctt tccgaaagcc
    15541 tctggttcgg cggtcatact ggttaaagcg atggtgtcat ttgggcaaat gttctaccca
    15601 atgctggtga gctatatgtt gctcaataat atctggtacg gctatgggct gattattccg
    15661 ggtattctgt ttgtactgat cacgctgatg ctgttgaaaa gcaaattccc cagccagttg
    15721 gtggacgcca gcgtagctaa tgaattaccg caaatgaaca gcaaaccgtt agtctggctg
    15781 gaaggtgttt catcggtact gttcggtgta gccgcattct cgacctttta tgtgattgtg
    15841 gtgtggatgc ccaaatatgc gatggctttt gctggtatgt cagaagctga ggcattaaaa
    15901 accatctctt attacagtat gggctcgttg gtctgtgtct ttatttttgc cgcactactg
    15961 aaaaaaatgg tccggcccat ctgggctaat gtatttaact ctgcactggc aacaataaca
    16021 gcagccatta tctacctgta cccttctcca ctggtgtgta atgccggcgc ctttgttatc
    16081 ggtttctcag cagctggcgg cattttacag ctcggtgttt cggtcatgtc agagtttttc
    16141 ccaaaaagca aagccaaagt caccagtatt tatatgatga tgggtggact ggctaacttt
    16201 gttattccgc tgattaccgg ttatctgtcg aacatcggcc tgcaatatat cattgttctc
    16261 gattttactt tcgcgctgct ggccctgatt accgcaatta ttgtttttat ccgctattac
    16321 cgcgttttta ttattcctga gaatgatgtg cggtttggcg agcgcaaatt tagcacccgg
    16381 ttaaacacaa ttaagcatag aggttaaagg agttaattat ggatgttacc gcaaaatacg
    16441 aattgattgg gctgatggcc tatcctatcc gccacagttt atcgcctgaa atgcagaata
    16501 aagccttaga aaaagcggga ttgccattta cctatatggc ctttgaagtg gataacgata
    16561 gttttcccgg agcaattgaa ggattaaaag ccctcaaaat gcgcggaact ggtatatcaa
    16621 tgccgaacaa acaactggcg tgtgaatatg ttgatgaatt aacgccagca gccaaactgg
    16681 tgggtgccat caacaccatc gttaatgatg atggctacct gcgcggctat aacaccgacg
    16741 gcacgggcca tattcgcgcc attaaagaga gcggttttga tatcaaaggc aaaacaatgg
    16801 tgctgttagg ggccggaggt gcctcaacgg cgattggcgc gcagggggca attgaaggtt
    16861 taaaagaaat taaactcttt aaccgtcggg atgagttctt cgataaagcc ctcgccttcg
    16921 cgcagcgggt taacgaaaac accgattgtg tcgtcacggt taccgatctc gccgatcagc
    16981 aagcctttgc tgaagccctg gcttccgccg acattttaac caatggcaca aaagtgggta
    17041 tgaaacccct tgagaatgaa tcattagtta atgatatcag tctgttacat ccgggactgc
    17101 tggtcactga atgcgtgtat aacccgcata tgacgaagtt attgcagcag gcgcaacaag
    17161 ctggttgcaa aacgattgat ggatacggca tgttgttgtg gcaaggggct gaacagttca
    17221 cgttatggac tggcaaagat ttccctctgg aatatgttaa acaggtcatg gggttcggtg
    17281 cctgacaggc tgaccgcgtg cagaaagggt aaaaaatgaa aaccgtaact gtaaaagatc
    17341 tcgtcattgg tacgggcgca cctaaaatca tcgtctcgct gatggcgaaa gatatcgccc
    17401 gcgtgaaatc cgaagctctc gcctatcgtg aagcggactt tgatattctg gaatggcgtg
    17461 tggaccactt tgccgacctc tccaatgtgg agtctgtcat ggcggcggca aaaattctcc
    17521 gtgaaaccat gccagaaaaa ccgctgctgt ttaccttccg cagtgccaaa gaaggcggcg
    17581 agcaggcgat ttccaccgag gcttatattg ctctcaatcg tgcagccatc gacagcggcc
    17641 tggttgatat gatcgatctg gagttattta ccggcgatgt tcaggttaaa gaaaccgtcg
    17701 cctacgccca cgcgcatgat gtgaaagttg tcatgtccaa ccatgacttc cataaaacgc
    17761 cggaagccga agaaatcatt gcccgtctgc gcaaaatgca gtccttcgac gccgatattc
    17821 ctaagattgc gctgatgccg caaagtacca gcgatgtgct gacgttgctt accgcgaccc
    17881 tggagatgca ggagcagtat gccgatcgtc caatcatcac gatgtcgatg gcaaaaactg
    17941 gcgtaatttc tcgtctggct ggtgaagtat ttggctcggc ggcaactttt ggtgcggtaa
    18001 aaaaagcctc tgcaccaggg caaatctcgg taaatgattt acgcacggta ttaactattt
    18061 tacaccaggc ataagcaata atatttcggc gggaacaccc tccccgccga acttaaaaaa
    18121 tatatttaac cgtatttaat aaaaatattt cgtgagtctc tgtgcgctaa ttctccatct
    18181 ggcgtaggga aaatcacatc tgaatcagga attaacaatg aaacctgtaa aaccacctcg
    18241 tattaatgga cgagtgccgg tcctgtcggc acaggaagcg gtgaattata ttcccgacga
    18301 agcaacactt tgtgtgttag gcgctggcgg cggtattctg gaagccacca cgttaattac
    18361 agctcttgct gataaatata aacagactca aacgccacgt aatttatcga ttattagtcc
    18421 gacagggctt ggcgatcgcg ccgaccgtgg tattagtcct ctggcgcaag aaggtctggt
    18481 gaaatgggca ttatgtggtc actggggaca atcgccgcgt atttctgaac tcgcagaaca
    18541 aaataaaatt attgcttata actatccaca aggtgtactt acacaaacct tacgcgccgc
    18601 cgcagcccac cagcctggta ttattagtga tattggcatc gggacatttg tcgatccacg
    18661 ccagcaaggc ggcaaactga atgaagtcac gaaagaagac ctgattaaac tggtcgagtt
    18721 tgataacaaa gaatatctct attacaaagc gattgcgcca gatatcgcct tcattcgcgc
    18781 taccacctgc gacagtgaag gctacgccac ttttgaagat gaggtgatgt atctcgacgc
    18841 attggttatt gcccaggcgg tgcacaataa cggcggtatt gtgatgatgc aggtgcagaa
    18901 aatggttaag aaagccacgc tgcatcctaa atctgtccgt attccgggtt atctggtgga
    18961 tattgtggtg gtcgatccgg atcaaagcca actgtatggc ggtgcgccgg ttaaccgctt
    19021 tatttctggt gacttcaccc ttgatgacag caccaaactt agcctgcccc taaaccaacg
    19081 taaattagtt gcgaggcgtg cattattcga aatgcgtaaa ggcgcggtgg ggaatgtcgg
    19141 cgtcggtatt gctgacggta ttggcctagt cgcccgggaa gaaggttgtg ctgatgactt
    19201 tattctgacg gtagaaacag gtccgattgg cggaattact tcacagggta tcgcctttgg
    19261 cgcgaacgtg aatacccgcg ccattctgga tatgacgtcc cagtttgatt tttatcacgg
    19321 tggtggtctg gatgtttgtt atttgagttt tgctgaagtc gaccagcacg gtaacgtcgg
    19381 cgtgcataaa ttcaatggca aaatcatggg taccggtgga tttattgata tcagtgccac
    19441 ctcgaagaaa attatttttt gcggcacgtt aactgcgggc agtttaaaaa cagaaattgc
    19501 cgatggcaaa ttgcatatcg tccaggaagg acgggtaaat aaatttattc gggaactacc
    19561 ggaaattact tttagcggaa aaatcgcgct cgaacgggaa ctggatgttc gttacatcac
    19621 cgaacgtgct gtattcacgc tgaaagaaga cggcctgcat ttaatcgaaa tcgcccctgg
    19681 cgtcgattta caaaaagata ttctcgacaa aatggacttc accccagtga tttcgccaga
    19741 actcaaactg atggacgaaa gattatttat cgatgcggcg atgggttttg tcctgcctga
    19801 agcggctcat taacaggagt ataatgatgg atttttcttt aactgaagaa caagaactgc
    19861 tgctggccag tattcgcgaa ctgattacga ctaactttcc ggaagagtat ttccgcacct
    19921 gcgatcaaaa cgggacatat ccgcgtgagt ttatgcgggc gctggcggat aacggtattt
    19981 ccatgcttgg cgtgccggaa gaatttggtg gtatccctgc ggattacgtc acccaaatgc
    20041 tggcgctgat ggaagtgtca aaatgcggtg ctccggcatt tttgatcacc aacggtcaat
    20101 gtattcacag tatgcgccgt ttcggttctg cagagcagct acgtaaaacg gcagaaagca
    20161 ccctggaaac gggtgatccc gcctatgccc tggcgttgac ggaaccaggt gctggctcag
    20221 ataacaacag tgccactacc acttacacgc gtaaaaacgg caaggtctac atcaacggac
    20281 aaaaaacctt tattaccggt gcgaaagagt acccgtatat gctggtgttg gcgcgcgatc
    20341 cgcaaccgaa agatcccaaa aaagccttca ccctgtggtg ggtcgactcc agtaagcccg
    20401 gcattaagat taacccgctg cataaaatcg gctggcatat gctcagcacc tgcgaagtct
    20461 atctcgacaa cgtggaggtt gaagagagcg acatggtggg cgaagaagga atgggtttcc
    20521 tcaatgtgat gtacaacttt gagatggagc gcctgatcaa cgccgcgcgc agcaccggct
    20581 ttgccgaatg cgcctttgaa gatgccgccc gctatgccaa ccaacgtatc gcttttggta
    20641 agcccattgg tcataaccag atgatccagg aaaaactggc gctgatggcg attaagatcg
    20701 acaacatgcg caacatggtg ctgaaagtgg catggcaagc cgatcagcat cagtcactgc
    20761 gcaccagcgc ggcgctggca aaactgtact gtgcacgtac cgcaatggaa gtcattgatg
    20821 atgcgattca aatcatgggc ggtctgggct ataccgatga ggcgcgcgtc tcccgcttct
    20881 ggcgtgatgt ccgttgtgaa cgtatcggcg gcggtacaga cgaaattatg atttacgttg
    20941 cgggtcggca gatcctgaaa gattatcaga acaaataatc tgcaggcggc gcagcttctt
    21001 aacaaactgc gccgccagat ttacccggca agacttaccg gttgaggaaa ttcagatagg
    21061 atttcttgtt ccgcaaaaca gtttttaaat tgcctgcggt aatcgctggg cgaacaaccg
    21121 acatggcgca aaaacagttt ggcaaagtga tcgacatttt cataacccac acgccaggag
    21181 atctctgcct gtgataattc agtattagtg agtgaccatt tcgcttccgt catacgccgt
    21241 tgaataacat agttaatagg ggaaatacga tactctttgg taaattcatg gcaaatataa
    21301 ctgacgctgg cacgaaattt tttcgataac tgttctaaag tgattttttc gcgataatta
    21361 ttattcagat aaaaaagaat atcttttatc agaacatctt ttttaatata cccctgctcc
    21421 gaacgataag catttttaaa gttttcgtag taaagaatgg ctaacgtata ggcaaatgca
    21481 tcccataacg aagatgtttg gctattttta ctttgcggca aaatcacact tagctcatta
    21541 aataaggttt ttatgacttc ttttccctgc cccgcggcaa ttaccggaca agaatgcggt
    21601 tgcagtagct gattttcctc agccccctga aactgaaagc cgtacagcgc acaggtacac
    21661 gtcgttgccg gatcgttaac gtcagaggcc accgcatgca gcctgccgcg ttctatcacc
    21721 actatatcat cggcatgtgc gacatataac gatgaatcga tggtaaacct tgctacccct
    21781 ttcttgacgt aaatcagctc ggtttcattg tcatgaacgt gatgtccaga ctcccatttt
    21841 ggatcatcgc taattgcaaa acgtgaaagc cgtggcgttt taccggcgac aaacagcgtt
    21901 tcactggcat tatcaaaaca gcgttgatac atgacaacct ccctattcca tgagcaagca
    21961 aaaacaatat atgccggata aaatacggcg tgtattgcgg ttatacaacc gcgtttagcg
    22021 agagctaaac aagattattt acactgaatg caagattgta cggtcatgga ataactatat
    22081 tgccgcaaat tgcacttttg ttttagcgat cgcatttttt ttgcaagatt gttggcaagg
    22141 aaaacagctt gctccgtcga aaaccccgca ccgctatcgc acactatttt caggccattt
    22201 ttaccttcca tcggagatgg ttccgtatgc gactcacagg agaaatcatg aaaataataa
    22261 cctgctttaa gctggtgcct gaagaacagg acattgttgt cactccagaa tacaccctga
    22321 atttcgacaa tgccgacgcc aaaatcagcc agttcgatct caatgccatt gaagctgcaa
    22381 gccagcttgc aacagatgac gatgagatag ccgcgctgac cgttggcggc tcattgttgc
    22441 agaactcgaa agtgcgcaaa gacgtgctat cccgcgggcc gcacagcctg tatttggtgc
    22501 aggatgcgca acttgaacat gcactgcctc tcgataccgc aaaagcgctg gcagcagcag
    22561 ttgaaaagat cggcttcgat ttactgatct ttggtgaagg ttccggcgac ctttatgccc
    22621 agcaggctgg cttgctggtc ggagaaattc tgcaacttcc ggtgattaat gcagtgagtg
    22681 ctattcagcg tcagggcaat acactggtga ttgaacgcac gcttgaagat gatgttgaag
    22741 ttattgaact ctctgttcca gccgtgctct gcgtcacctc cgatattaac gtgccacgca
    22801 ttccttcgat gaaagccatt ctcggcgcgg gtaaaaaacc ggtaaatcag tggcaggcaa
    22861 gtgatattga ctggagccag agcgcgccac ttgctgaact ggtcggcatt cgcgtaccgc
    22921 cgcaaacaga acgtaagcac atcattatcg ataacgattc gccggaggcc attgccgagc
    22981 tggcggaaca tctgaagaaa gccctgaact gaagcctacg gagaagaaac gatgagtcaa
    23041 ttaaacagcg tctgggtctt tagcgataat cctgaacgtt atgctgaact gtttggcggc
    23101 gctcagcaat ggggccaaca ggtgtatgcc attgtacaaa ataccgacca ggcgcaggca
    23161 gttatgcctt atggtccaaa atgtatttat gttcttgagc aaaacgacgc gctgcaacgc
    23221 actgaaaatt acgccgaaag cattgctgcc ctgctgaaag ataaacaccc cgctatgttg
    23281 ctgttggccg cgacgaaacg tggtaaagct ctggcagcac ggttaagtgt gcaactgaat
    23341 gcggcgctgg tgaatgatgc cacggcggtg gatattgtcg atggtcacat ttgcgccgaa
    23401 caccggatgt atggcgggtt agcgttcgct caggaaaaga tcaacagccc gctggcgatc
    23461 attacccttg cacccggtgt tcaggaaccg tgcaccagtg atacctctca tcagtgcccg
    23521 acagaaacgg taccttatgt tgctccgcgt catgaaattc tctgtcgcga acgccgtgcg
    23581 aaagccgcaa gcagcgtgga cctgagcaaa gcaaaacgtg tggttggcgt cggtcgtggt
    23641 ctggcggcgc aggatgacct gaaaatggtc cacgaactgg cggcggtgct gaatgctgaa
    23701 gtcggctgtt cacgtccaat tgccgaaggc gagaactgga tggagcgtga acgttatatc
    23761 ggtgtctccg gcgtgttgct gaaatccgat ctctacctga cgctggggat ctccgggcag
    23821 atccagcata tggttggcgg caacggcgca aaagtgattg tcgccatcaa taaagataaa
    23881 aatgcgccaa tcttcaatta tgccgactac ggtctggtgg gcgatatcta caaagtcgtc
    23941 cctgccctga ttagccagtt gagccgctaa ttcccctttc aaccacatct cccgctgtaa
    24001 acgcagcggg aagggagcat aacgcatgtc ggaagacaaa tttgatgcca ttgtggtcgg
    24061 tgcgggcgtt gctggtagcg ttgccgcact ggtcatggcg cgagccgggc tggatgtcct
    24121 ggtgatagaa cgcggcgaca gtgccggatg taaaaacatg accggcgggc gtctttatgc
    24181 ccacacactt gaagcaatca ttccaggctt tgcagtatca gcgccggtag aacgcaaggt
    24241 cacacgcgag aaaatctcct tcttaaccga agaaagcgcc gttaccctcg attttcaccg
    24301 cgagcaacca gatgttccgc aacacgcatc ttataccgta ttgcgtaatc gtctggaccc
    24361 gtggttgatg gaacaagccg agcaggcggg cgcgcagttt atcccaggcg ttcgcgtcga
    24421 tgcgctggtt cgtgaaggaa acaaggtcac tggcgtgcag gctggggatg atattctcga
    24481 agcgaatgtg gtgattctgg ctgatggcgt taactcgatg cttggccgct cgctgggaat
    24541 ggttcccgct tccgatccgc atcattacgc tgttggtgtt aaagaggtta ttggcctcac
    24601 accagaacag atcaacgatc gctttaatat tacgggcgag gaaggtgccg cctggctgtt
    24661 tgccggttcc ccttctgacg gcctgatggg cgggggattt ctctatacca acaaggattc
    24721 catatccttg gggctggttt gtggattggg tgatatcgcc catgcgcaaa aaagcgtgcc
    24781 gcaaatgctg gaagatttta aacaacaccc cgccattcgc ccactaatta gcggcggcaa
    24841 actgcttgaa tattccgggc atatggtgcc agaaggcggt ctggcaatgg tgccgcagat
    24901 ggttaacgat ggcgtgatga tcgttggtga cgccgcaggc ttctgcctga atttgggttt
    24961 tacagttcgt ggcatggatt tagccattgc atcggctcag gctgccgcca caacggtgat
    25021 cgccgccaaa gaacgcgagg atttctccgc cagcagtctg gcgcaataca aacgtgagct
    25081 ggaacaaagc tgcgtcatgc gcgatatgca gcatttccgc aagatcccgg cgctgatgga
    25141 aaacccgcgc ctgtttagcc aatacccacg aatggtcgcc gacatcatga acgagatgtt
    25201 caccattgac ggcaaaccaa accagccggt acgcaaaatg atcatgggac atgcgaagaa
    25261 aattgggctg atcaacttgc tgaaagatgg cattaaggga gcaaccgcgc tatgagccag
    25321 aacgctacgg ttaacgtcga catcaaatta ggcgtcaata aattccatgt tgatgagggc
    25381 cacccgcata tcattctggc ggcaaatccc gatatcaatg aattccgtaa attaatgaaa
    25441 gcctgccctg ccggacttta taagcaggat gacgccggca acattcattt tgattctgcc
    25501 ggttgtctgg agtgcggcac ctgtcgggtg ctgtgcggta acactattct cgaacagtgg
    25561 caatatcccg caggcacctt cggtattgat tttcgctacg gctaatcatg catcccacag
    25621 gcccgcatct cgggcctgat gttctgtttc gagagtccaa catgaaagtg acattaacgt
    25681 tcaacgaaca acgtcgtgcg gcgtatcgtc agcaagggct atggggcgat gcttcgctgg
    25741 ccgattactg gcagcagacc gctcgtgcga tgccagacaa aattgccgtg gtcgataatc
    25801 atggtgcatc gtacaactat agcgcgctcg atcacgccgc gagctgtctg gcaaactgga
    25861 tgttagcgaa gggtattgaa tcaggcgatc gcatcgcatt tcaactgcct ggctggtgtg
    25921 aatttaccgt tatctatctt gcctgcctga aaatcggtgc ggtttccgtg ccgctgttgc
    25981 cttcctggcg ggaagcagaa ctggtgtggg tgctcaataa gtgtcaggca aaaatgttct
    26041 ttgcaccgac gttgtttaaa caaacgcgcc cggtagattt aatcctgccg ctgcaaaatc
    26101 agcttccaca actacaacaa attgtcggcg tggacaaact ggctcccgcc acctcttccc
    26161 tctcattaag tcagattatc gccgacaata ccccactgac cacggcgata acgacccacg
    26221 gcgatgaatt agctgcggtg ctgtttacct ccggaaccga gggtctgcca aagggcgtga
    26281 tgctaacgca taacaatatt ctcgccagtg agcgggctta ttgcgcgcgg ctgaatctga
    26341 cctggcagga tgtctttatg atgcctgcgc cactcggcca cgcaacgggc tttctgcatg
    26401 gcgtaacggc accattctta attggcgctc gcagcgtgtt gttagatatt ttcactcctg
    26461 atgcgtgtct cgcgctgctt gagcagcagc gttgcacctg tatgctaggc gcaacgccgt
    26521 ttgtctatga tcttttgaat gtactagaga aacaacccgc ggacctgtca gcgctgcgtt
    26581 tctttctttg cggcggaacc acaatcccca aaaaagtggc gcgtgaatgc cagcagctcg
    26641 gcattaaatt attaagtgtt tatggttcca cagaaagttc gccgcatgcg gtggtgaatc
    26701 tcgatgatcc tttgtcgcgc tttatgcaca ccgatggtta cgctgccgca ggtgtagaga
    26761 ttaaagtggt cgatgacgca cgcaagacct taccgccagg ttgcgaaggt gaagaagcct
    26821 cgcgtggccc caatgtgttt atgggatatt ttgatgaacc tgaattaacc gcccgtgcgc
    26881 tggatgaaga aggctggtat tacagcggcg atctctgccg tatggatgag gctggctata
    26941 taaaaattac cggacgcaaa aaagatatta ttgtccgcgg cggcgaaaat attagcagcc
    27001 gtgaagtgga agatatttta ttgcagcatc ctaaaattca cgatgcctgt gtggttgcaa
    27061 tgcccgatga acgtttaggt gaacgatcat gcgcttatgt cgtgctgaaa gcgccgcatc
    27121 attcattatc gctggaagag gtggtggctt tttttagccg taaacgggtc gcaaaatata
    27181 aatatcctga acatatcgtg gtaatcgaaa aactaccgcg cactgcctca ggtaaaatac
    27241 aaaagttttt gttaagaaaa gatattatgc ggcgattaac gcaggatgtc tgtgaagaga
    27301 ttgaataagt ttcatcttcg gggatcacat aaacccggcg actgaacgcc gccggggatt
    27361 tattttattt cttcagttca gccaggctta accaggtttg caccacggtg tccgggttca
    27421 gagacaggct atcgatcccc tcttccatca accatgcggc aaagtcttca tggtcggacg
    27481 gaccctgacc gcaaatcccg acatatttgc cctgtttctt cgcggcacga atcgccatcg
    27541 acagcagtgc tttcaccgca tcgttgcgct catcgaacag ttcagacacc actccggagt
    27601 cacggtccag accgagcgcc agctgtgtca tgtcgtttga gccaattgag aagccgtcga
    27661 aatattcgag gaactgctct gccagcaagg cgttggacgg gatttcacac atcatgatga
    27721 ttttcagccc gttctcgcca cgtttcagcc cctgacgcgc cagttcttca accaccgctt
    27781 tcgcctgatc tacggtacgc acgaacggga tcatgatctc aacgttggtc agccccatgt
    27841 cattgcgcac acgtttcact gcttcacact ccagcgcgaa acagtcgcgg aagctgtcgg
    27901 aaacatagcg gcccgcgcca cggaagccga gcatcgggtt ctcttcatct ggctcgtaac
    27961 gctcaccacc gaccaggttg gcatattcgt tcgatttaaa atcagagaga cggacaatga
    28021 cgcgcttcgg ataaaacgcg gcacccagcg tcgcgatccc ttcagtcaga cgaccaacgt
    28081 aaaattcacg cggagaatca aaacctttca tcatctcgcg gatttcgttt tgcaactgcg
    28141 gttcctgatc gtcaaactca agcagtgcgc gtgggtggac gccaatcata cggttgatga
    28201 taaattccag acgcgcaagg cccacgcctt cgttcggcag acaggcgaag tcgaaagcac
    28261 ggtccgggtt accgacgttc atcatcactt tcaacggcag atccggcatc gtttctacgc
    28321 tggagctttt cacgctaaat tccagcaact ccgcatagac gtaaccggta tcaccttcgg
    28381 cacaagaaac agtgacgttc tcaccgtctt tcatccgttc tgttgcatct ccacagccca
    28441 ctaccgccgg aatgcccagt tcacgagcga tgatcgccgc gtgacaggta cgaccgccac
    28501 ggttggtgac gatggcagat gctttcttca tgatcggttc ccagtccggg tcggtcatat
    28561 cagtgaccag cacgtcacca ggttcgatgc ggttcatttc gctgatgtca tggatgactt
    28621 tcaccggacc cgcaccgatg cgatgaccga tagcacggcc ttcggcgata atcttaccct
    28681 gtgaatgcag cgtataacgc tccatgacct gaccgcgtga gcgcacggtt tccggacgcg
    28741 cctgcacaat gaacagtttg ccggtgtggc catctttcgc ccactcaata tccattgggc
    28801 gaccgtagtg tttctcaatt tgtacggcct gttttgccag ttcctgtact tcttcgttgg
    28861 tcagcgagaa gatgtcacgc tgttcctgcg gtacgtcttc gattttaacc tgcttgccgt
    28921 gctcctgggt cggcgcataa accatgcgga tttttttcga ccccatggtg cggcgcacga
    28981 tagccgggcg attcgccgcc agtgtcggtt tatggacgta aaactcatcc gggttaaccg
    29041 caccctgcac gaccatttcc ccaaggcccc atgcggaagt gataaacacc acctggtcaa
    29101 agccggattc ggtatcaatg gagaacatca cgccagaaga tgcgaggtcg gagcgcacca
    29161 tccgctgaac accggcggag agcgccacgc cacggtgatc gtaaccctgg tgcacacgat
    29221 aagagatggc gcgatcgtta aacagagaag caaatacatg tttcactgcc acgagaacgg
    29281 cgtcaaaacc ctgaacgttg aggaaggttt cctgctgacc ggcaaaagaa gcgtccggca
    29341 tatcttctgc agtagcggag gaacgcaccg caaacgaggc gttttcgtca tcggcggaaa
    29401 gctgtgcata ggcttcgcgg atggcgtttt ccagctcagg ctggaaggga gtgtcgataa
    29461 tccactggcg gatttgcgcg cccgctttcg caagctgagt aacatcgtca atatccgttt
    29521 tatccagcag ttcataaatg cgctggttta cgccgctttg gtccagaaac tggttaaacg
    29581 cgtcggcggt tgtggcgaaa ccattcggaa cggaaacacc cattccggaa aggttagtaa
    29641 tcatttcacc cagggaggca tttttgcccc caaccctgtc tacatcattc atgccgagtt
    29701 ggttatacca aagcaccagc ggtgacgagc cattgttgga catcgaacaa tccttttgtg
    29761 ataaatgaac ggtttgagaa acacatttct gcgcatttat ctttgcatat ttaaccggat
    29821 gaaaaaaacg gtgaatcgtt caagcaaata tattttttta ctttttaaga ctgatcccag
    29881 cgttgcgcaa atctgctctc ttcgacaatt tccacataaa ccatcggtat aaacgaaacc
    29941 ataaaaatga aatactgttt tcataaaaaa taaaattgaa ggttcatttt ataaaccaga
    30001 cattacgtcc tacgcttctg tgcgttttta atttatgctt tcatagaatt atgtctgcat
    30061 cacgggaaga acaaaatgga taatgctgtt gatcgccacg ttttttatat ttctgatggt
    30121 acggcaataa ctgcggaggt attaggacac gcagtaatgt cacaatttcc cgtcactatc
    30181 agcagcatta cgctgccgtt tgtcgaaaat gagagccgtg cacgggcagt gaaggatcag
    30241 attgacgcca tttatcacca gacaggtgtg cgcccgctgg tcttctactc catcgtgttg
    30301 ccggagattc gcgccatcat cttgcaaagt gaaggctttt gccaggatat cgttcaggcg
    30361 ctggttgccc cgctacaaca agagatgaaa ctggatccaa cgccgattgc tcatcgtacc
    30421 catggcctta accctaataa tctcaataaa tatgatgcgc gcattgcggc gattgattac
    30481 acccttgccc acgatgacgg tatttcgttg cgcaatctgg accaggctca ggtgatcctg
    30541 ctcggcgttt ctcgctgtgg taaaaccccc accagtctgt atctggcaat gcaatttggt
    30601 atccgcgcgg caaactaccc ctttattgcc gacgatatgg ataatctggt gctacccgcg
    30661 tcgctcaaac cgcttcagca taaattgttc ggcctgacta tcgacccgga acgtctggcg
    30721 gcgattcgcg aggaacgtcg ggagaacagc cgctatgcct cgcttcgtca gtgcaggatg
    30781 gaagtcgcgg aagtagaagc gttgtaccgt aaaaatcaga tcccgtggat taacagtacc
    30841 aattattcgg tagaagagat tgccaccaag atcctcgata tcatggggct tagccgccga
    30901 atgtactaga gaactagtgc attagcttat ttttttgtta tcatgctaac cacccggcga
    30961 ggtgtgacac acctcgcact tgaaatcagc tgcgattggt ttatcgtgat gcgcatcact
    31021 tcccggtagt cctgccgtag aagcaacaaa tttctgagac ttgtaatgaa cagaactgac
    31081 gaactccgta ctgcgcgtat tgagagcctg gtaacgcccg ccgaactcgc gctacggtat
    31141 cccgtaacgc ctggcgtcgc catccatgtc accgactccc gccgcagaat tgaaaaaata
    31201 ctgaatggtg aagataagcg actgttggtt attattggcc cctgctcgat ccacgatctc
    31261 accgctgcaa tggagtacgc cacccgtctg cagtcgctgc gcaaccagta ccagtcacgg
    31321 ctggaaatcg taatgcgcac ctattttgaa aaaccacgaa ctgttgtcgg ctggaaaggg
    31381 ctaatctccg atccggattt aaacggcagc tatcgggtaa atcacggtct ggagctggcg
    31441 cgcaaattac ttttacaggt aaatgagctg ggtgtcccta ccgcgactga gttcctcgat
    31501 atggtgaccg gtcagtttat tgctgattta atcagttggg gcgcgattgg cgcacgtact
    31561 accgaaagtc agatccaccg tgaaatggct tcggcactct cctgtccggt aggttttaaa
    31621 aatggtaccg atggcaacac gcggattgcc gtggatgcta tccgcgcagc ccgcgccagc
    31681 catatgtttc tctcgccaga caaaaatggt cagatgacca tctatcagac cagcggcaac
    31741 ccgtatggcc acattattat gcgtggcggc aaaaaaccga attatcatgc cgatgatatc
    31801 gccgcagcct gcgatacgct gcacgagttt gatttacctg aacatctggt ggtggatttc
    31861 agccacggta actgccagaa gcagcaccgt cgccagttag aagtttgtga ggatatttgt
    31921 cagcaaatcc gcaatggctc tacggcgatt gctggaatta tggcggaaag tttcctgcgc
    31981 gaaggaacgc aaaaaatcgt cggcggtcag ccgctcactt acggtcaatc cattaccgac
    32041 ccgtgtctgg gctgggaaga taccgaacgc ctggtcgaaa aactcgcctc tgcggtagat
    32101 acccgcttct gaatgcgtgc ccattcctga tgggatgggc atttctgcgc aacttgttgt
    32161 cttttcaaca cattactgct tgctctggcc tgccataata ttgataataa gaatcattgt
    32221 tatatcaatt attattaatt tttatgcgtt atacggatag cagaaaactc acgcctgaaa
    32281 cggatgccaa tcacaagacc gcttccccgc agcccatccg gcgaattacc agccagacac
    32341 tgttaggtcc ggatggcaaa ctgattatcg atcatgacgg gcaagaatat ctgctccgta
    32401 aaactcaggc tggcaagctg ctgttgacca agtagccttt aactcgagca gctgacttcc
    32461 agccgtttac cccagtcagg tggacgactg acatagtcat cagctctgtc gctgaaagga
    32521 tttcgcaacg cctcatgcag gcggtgcaat tccgtcatat cacccttttc tgccgcttca
    32581 atcgcccgtt gcgccaacca gttgcgcaac accagagctg ggttaacgct ttgcatcagt
    32641 tgctgacgct caatatcgct aacctcgtct tgttgcaaac gcctccgata acgggcaaac
    32701 cagtcatcaa atgccgcacg atcaataaac tcatcacgta gcggtgacgc cgcgctgtgc
    32761 tgctcggtca gactcagcat gcggaatgtg cgggtataat cgctgcgctc tcgcgccatc
    32821 agactgaata attcattcag tagcgcgtta tcctcttttt gctccgtcat gaagcccagt
    32881 ttctgccgca tccgttgtcc ataatgcgtc aacaaaacct gctgatagct gtccagggcc
    32941 tcattcaggg catctacggc aacaaatggt gacaatgtct gcgccagacg ctgtaaattc
    33001 cacaacgcga cggcaggttg attatcaaag ctgtaacgcc cttgatgatc cgagtgatta
    33061 caaataaaac cgggttcgta atcatcaaga aaaccaaacg gcccgtaatc aagcgtcagc
    33121 cccagcagcg acatgttgtc ggtattcatc accccatgag caaagccgac cgtctgccat
    33181 tgggcaatta acgatgcggt acgtgcgaca acatcgttaa accagagacg gtatttatcc
    33241 tcatcatctt caagatgtga ccagtaatga cgaatggcaa agtcagctaa ctgacgaacc
    33301 ttttccggct cgcggcgata gtaaaaatgt tcgaaatgac caaagcgcag atgacttggt
    33361 gccacacgca tcagcatcgc acctggctct accgtttccc gatacactgg cgaatcgctg
    33421 gtgacgatac ttaacgcgcg ggtcgtcgga atgcccagat aatgcatcgc ctcactggcg
    33481 agactttctc gtatcgtcga acgtaaaacc gcccgtccat cacccattcg cgaataaggc
    33541 gtcaggccag cacctttcag atgccagtcc attgtagtgc catcagcaag cagttgttcg
    33601 ccaagtaaaa tgccgcgccc atcacccagt tggcccgccc agacgccgaa ctgatgacca
    33661 ctgtaaacct gcgccagtgg tgacatacca ggcagtaggg tttcgccgcc ccagacacct
    33721 gcagcatttt taaacagcga cgatggaata ctcagcgtgt tagcgagttc ggtattatgc
    33781 caaatcagcc gggcattatt taaaggcgta ggggaaagtg ctgtataggt ttctggcaat
    33841 tcatcgcgcc agcgggtaat aaaagacagg gtcatagatc ctcctgtttg atagtgtaga
    33901 cggttactct cgtcttaaac accagcaaac aggagggtta tcgctgaacc aacgtcgtta
    33961 tctgatttat aggaacggca ggccacaggc atccctggag agcatggaaa tcgaaggggg
    34021 taatctgtgc caatatttct gtcgtatcaa tgccccccgc gatgatgcaa ttgcaacaag
    34081 gggaaatttg cgcctggatc gcacgaataa acggttcgaa ggagcgatgc gtgatttgtt
    34141 gctgaataaa acttttatcc agcatgacgc gggtgaaaag accatcaaaa accgctttca
    34201 ttgtgctatt acctgctccc aaattgccta acacaagggg atacatttgc gataaggata
    34261 aaagatcgcg attatctttt ccttcattga gatgcgggta attctcatta atcaacagtt
    34321 caataaacgg atattttaat agttcgcctg cgtaattatc cctctctaat aataatgtag
    34381 ccacttgtgg cgttaaatta agccaggcaa agagtttatg ttgaataaaa aaatgttggc
    34441 atgattttag taattccaac tgctctgaaa ataattgcca atgttgctcc tcggtaagtt
    34501 gcgctataac ccgactcgtc gggatacgaa ccgtaccatc ttcactggag aaatgggtaa
    34561 ttaactcaac gccgacaagg tcttgctggt tatctcttat cggcaggaaa tagcaatcag
    34621 aatgataaag attctccaaa aaaatcttca tggtaaccgt ccctctcgaa ggatgttttt
    34681 cagtatccgg ctaaaaggga tgaggccata agacataaca attacataag gagtaacttt
    34741 catttgttcc atgttaacca ctttttcagg ggtccttttt ttagattatc ctgattataa
    34801 acgaataatc catttacgga atttttgtct gcaaaatact actgtattca ggagtaaatg
    34861 gacggcgatg tatcatcgcc gtccattttt cgacatacta aattcgtctc gcctgccaga
    34921 aatttttctg ccaatagaca ttatcaagtg aggaacgcat cactcccttg ctggtagagg
    34981 cgtggataaa ttggttgttg gtatcgtaaa tacctacatg caaaccattt tgtccggaac
    35041 ccgttttgaa aaagaccagg tcaccaggca gcaactcgtc tttatcaatt tgcgtgccga
    35101 tagaggcttg ttctttggtt tctcggggca gctgcaaatc gaaacgatcg cgcatcgtta
    35161 caaccacaaa tcccgaacag tccacaccgc gccgcgtcat gccaccataa cgatacggcg
    35221 tgccatgcca gctttgtagc tggtcgttca aaccggcaat aacggtaatc gaatcagaaa
    35281 gtctggcatt tggcggcggt gctttatggt ggctacaccc ggccagaaac agtgctgtga
    35341 tcaaaataag gcagaaacgc attccgtacg gttcctctgt tttttattct tgcattaatt
    35401 tagcgtcgta attacccgat tttcaagata ctaatgaaat cagatggtcg aaatcagcat
    35461 tctgtgacct tcgatatcca gacggcgaaa attcatccca taggcctgcg ccagatttgg
    35521 cggcgtgagc acctcttctc tgcgtccact ggccagcatt tttccacctt ttagcaacca
    35581 cgcccgatgc gcatgacgca atgtgtggtt gagatcgtga ctgctcatca caatcgccag
    35641 tccttgctga cacagagcgc tcagaatttt gtctaacgca ctttgttgcg caacatcaag
    35701 actgttcatc ggctcatcaa gaagcagcaa ttgacctgcg ggattggctt gtggtgtgat
    35761 ttgcagcacc accgcagcaa gacgtacgcg ttgccattca ccgccggaaa gttgattggt
    35821 gctacgaccg agtttgtcat caagagccag cgcccctgcg acatcattca gtagttcggt
    35881 acgcgtttta tcgtgctgat gcagtgtcag gtagtgccag accggcattg caaacggcgg
    35941 cgtctgctgt tgtgaaagat aggcgcgatg cagcgcgagt tttgttgcgg accatgcttc
    36001 caggggttgc cccgcgaact gaatgcttcc cttaccgctg gtcattccgg ccattcgtgc
    36061 aagcaaggta ctcttacccg cgccattcgg ccccaccagg tgcaggatct ccccagcccg
    36121 aacctcgcca gaaagtggcc ccaggcgggt agattccgca acatcctgta actgcatcac
    36181 aatagacatt attttgccaa cgccagttta atgctttcca tcacaatggg gtcttccggc
    36241 gtcatatccg gggaaaaacg ctggatgacc aatccgtccc tgccaactaa aaatttctca
    36301 aaattccata aaatatcatc agggtacagc ggtgcacgac ctttgctgac catacgggca
    36361 tagaatccgc tctcttccgg cgcgactgcg gtcggcgctg cggcaatcaa tttttgatac
    36421 agcggatggc gtccttcgcc attaacttca atcttactga acatcgggaa cgtcaccccc
    36481 catgtggtgg tacagtaagt tttaatctct tcatcgctgc ccggttcttg ttccagaaac
    36541 tggttgcacg ggaatcccag caccacaaaa cctcgatcgg cccaggcttt ctgaatattc
    36601 tccaactgct catattgcgg cgttaagcca cactttgagg cgacattgac aatcaacagc
    36661 acattaccgg cgtacttctc cagcgtggtc acttcaccgt cgatatcttt cactacggtc
    36721 gtcagaatgg aatcttgcat cgtttctcct gggtgtggtc agtaaaaatc ttagctttta
    36781 atcatagacc gtctttttgc ggctaacgtc ctgcttttaa caataaccag ataaacaccg
    36841 gcgcacccaa cgttgcggtg accacgccaa taggcagctc tgcggcagct aatgccaagc
    36901 gcgctacaac atcggccagc agcaatgcgc tcgcccctgc cagcgcgcag ccgggaagta
    36961 atacgcgatg atcggttaaa ccacacaacc gaagaatatg tgggatcacc agaccaataa
    37021 agccgatagc acccgccagc gccacactga cgccaaccat ccagccggtc gctgccacca
    37081 gcacattgcg ccagaaccac aggggtaaac ccagttgccg cgccgagatc tcgccaagtg
    37141 ctaacatatt catcggcctg gactgacaac agatccacaa caacacgggg atcaatgcca
    37201 gcatcagcca gctttgccgc cagtctacgc cgccaaaacc gcccatcatc cagtacatca
    37261 gctgacgtaa atcaacagag gtggaaaagt agatagccca cgtcattagt gcgctacaga
    37321 taatccctaa tgcaacgcca gccagcaata accgactggt cgaaagatga cgacgggcga
    37381 aacgtaagag tattaaagtg atgataagcg cgccagcaat cgcacacagc cctagtgccc
    37441 agtttgggag ttgcccttgc ccaagcaata ccgcggcgat aagccctacg cctgcgccgt
    37501 tagagacgcc aagtagtcca ggttctgcca gaggattttc aaacaacgcc tgcattactg
    37561 cgccggatat agccagcgcc gcaccaacca gcaatacagc cagcgtacgt ggcaggcgaa
    37621 tctgccagac gaacagttcg ccacgaggag aaaaccagtc acctggcgag atccattgtt
    37681 caccggcgca aaggcttaag agaagcgcca gcagcatcaa aactgacagg cataataacc
    37741 agcgaatatt ttgtcgctgt tgttggcggg caagtgtcag catggtatcc gttctgctga
    37801 agtgtcatgg cgttgatttt acggtgactc ttcgacagtg aaaagaaaaa aggccgcaga
    37861 gcggccataa caacgaatta aaaacaataa cttaccgttt tatcaaacac ttacagacac
    37921 acaaaaaagc agacaatcac aacgaataac agcaatgtgg tcactttgtg gatcatatcg
    37981 ccaacaaaaa aaatcactta atggcaacca taatcgccac aagtgcagcc attgctgaca
    38041 caatgatact tgcaagctgc caggtctgac tggtaaatgc tttttgaatt tcagtacgat
    38101 gatcagccat ctcaactttc agcgactgac gtaaatcagc catctcaagc cgcaaccctt
    38161 cgcgaatctc cagtacatca gatttggtgg caaaggattc ggagcgggtc gtcaggatgg
    38221 ctatattgct tttaatatca gcgacgtcag cctcaagtct ttcaactcgc ttttccaatt
    38281 tgtcaggcat gacattacct ccattgttgc ctccacctac tacaaatgca ttataacatt
    38341 gagcgtgtct gcgcctcgta atattatctc tgggaggctc gtaacaccga ggaatcattt
    38401 ttcaccacca cgatgattta accagctcat gaaggcttgt gccgagtaac ggtggatgaa
    38461 cccacatctg ctacacataa ccttaaattc gtagttatga atatattccc gatcatgccc
    38521 gttataccct ggatacatgg tgcatggacc ggtgtaatca agttgaatgc gcgtgcctaa
    38581 attgcattct tcagccgatc ttggttcagg cccaacctcc acaacatcag acaagtgcat
    38641 atacatatct gtagagccac acgataagca tttttcattt gcagaagagt cattgaggaa
    38701 acgagcaaaa ttatcaggcg tcgccattct ttcaagttca tctaacggtt tggtcacgtt
    38761 taatcttctc ctagtgggtt aaacctcacc gcatcctgca aataatccgg cgcaagatgg
    38821 gcataaatca tcgttgtctg aatccttgcg tgccccagaa ttttctggag cgtcagaata
    38881 ttgccgccgt tcatcatgaa atgactggcg aaggtgtgac gcagtgcatg aacagcctga
    38941 ccgtcaggaa cgtcaggcgc gaccacctta atagcatcac gtacaagcgg gtaatccagc
    39001 accggaaaaa cccgttttcc gccccggttt ttaatttttt caaacagact ttcagagatc
    39061 ggaacagtgc gatttttact gttctttgtt ctggagaagg tgattcggca atgctgaacc
    39121 cgacgctgtt caagcgccac cacttccccc caccgcgcac cggtagacag gagtatttca
    39181 actgccagtc gttcatcagg atgttcagcc agggcatcca gcaaccggag acactccggt
    39241 ttacttaagt aacccatttc acgctcgaca accttcatcc ctttcagtcc ctgaacaggg
    39301 ttgtcgttaa gaaagtgaca ggatgagatg agtgcgctga acatcgagct aagagcgcca
    39361 acctcgcggt ttatagtgct tggctgaatc ccttcttccg ccctcgccac gcgcaactct
    39421 gttagcgtgg tcgcattgac tttatgaaca ccgggatcat tcataagctc gcacaggcgc
    39481 agcagcttca ggtgcgtatt tcgcccggac ctcattagct gcccatgata tttccaccac
    39541 agatcaataa gctctgacag cggccggcga tcggtgggtg cacttttcca ctcattgcta
    39601 tgttgttgcg acaacaccca tcgctcatac aaaacggcat ccgatttcgt tttaaatttt
    39661 ttacggatgc gcttaccttt gcgaccatca gggcgcatat caacaaggta accaccggga
    39721 attgatttta tactcatcag cacaccccca gcgtcacaag accaccatga ccccagcgct
    39781 ccataattaa ccgtgctgtg tgccagtttt gcggggtttt agagaagacg atgtgctttc
    39841 tggcccatca ggggagagag ccgggcttat ctgacctgcg gcctcgtttg tttttccggt
    39901 caacaaccaa ttcatgtact taaaaaagcg agggtgatta acaatcttaa cgagaacatc
    39961 actccctaca ttttcaatcc ggccagtctc ataacgacgc aaagtaccaa ttggcacatc
    40021 aatcaggccg caaaactctt cacgcgttag gtcctcaaat tcacgaatca ctctgatttt
    40081 ttcaccaata agcattgaca gtgttcctat aagtacacta agcttgcgca caaggtgtac
    40141 ttataagtac acaaaaggca caaacaacca cagatagaac aggttatcac acatggcaaa
    40201 aattcagaac gagcgcggac aagtggaaat tgagcgcttg atggctctct acccataccg
    40261 tgatgagcgt ggattgccga tacttgaaga aagccttaag gactacgcga aacggacaaa
    40321 ccaggctttt aacaccgtta aaagacaggc cgacagagcg caaatcccaa tcaaccagga
    40381 cgaaaaatac ggaaagcgaa cggtaaacct gtacgcgatt tttctgaaaa ccatcagaaa
    40441 cgcagagaaa tacgtacaga tgacgaaata acaggaagtg aaggtgacta tgaaaaatca
    40501 acgtaatgcc ggactacaca gaagcaaaga gcgcctcgtt caccgtcagg acttccgcaa
    40561 tacaaatact gaaatccacg ccacaccaga cggtcacccg gtaaaacaaa ctggcaagca
    40621 cacctggacg attggcgaca ctggcatcgt tattcacaaa agttttcgca gccctgttac
    40681 tggccgattt aattttactc tgactcgcgg agatgattac ttcggacagg acttcacatt
    40741 ttttgaagcc ttaagaacgg cagacagatt aatcaacggc ctgtgttttc agtatccggg
    40801 aagtaagcac tgataaataa aggattcagt catgatcgaa gcctacaaaa tacttattaa
    40861 taacgcgttg cagcgttacc acttcgaact tgcaaaccgg ggcgtaacta aggatgaaag
    40921 ccttcgtaac tcactgcgcg gcgcaattat gggactgtat aactgcgcgc tgggcagcga
    40981 tgaccgtgaa gcactggaag aattaaagtc gattatgaca gaactgtttg aaggtgaaat
    41041 tgttgagcct tacttcacag gagtggcagc atgagcatat tcctttcatg ggtaattctg
    41101 attatatccg tgcttatggc aatcggcatt atgcgaatta tccacacact gaaggatgtc
    41161 gaattatttt tcactggcga caaacgccgt aaataaagcg aaaagattgt gaagcacgtg
    41221 aaaacaataa tactgaatat cgccactgca tggttggtat ttcaggtgtg cgcacttaca
    41281 tggttagcac tgaagaatct ggggataatc tgaagccgaa agcagccacg gaataacacc
    41341 agaaaacata cgaacaatta attaaggcat tcatgccttc ggcttccctt tgtctttttt
    41401 caggagtcaa caaatgagaa ctgccgaatc acccagcaga gaaataaccg ccagaccgga
    41461 acagccacat gcacacatca gcatgaccgc aagaaaagcc gaacgtgcgc atatctgcga
    41521 attactgcgc gacctggtta acacggcgaa agagaaacac acctccccgc tggaaacatg
    41581 ttcagccatt gaacgactga ttgcaacgct cacgaacaac gccgaacaga aggagtgcac
    41641 acagtgaacc atctcatgat cgacaccgaa acactgggta ccggaccgga tgcagtcata
    41701 ttcgcgatcg g
//
LOCUS       DAZGVQ010000023        41441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000023 DAZGVQ010000000
VERSION     DAZGVQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.023"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            253..339
                     /gene="leuL"
                     /locus_tag="ACHLAG_001213"
     CDS             253..339
                     /gene="leuL"
                     /locus_tag="ACHLAG_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFP0654480.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            432..2003
                     /gene="leuA"
                     /locus_tag="ACHLAG_001214"
     CDS             432..2003
                     /gene="leuA"
                     /locus_tag="ACHLAG_001214"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFP0654481.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2003..3094
                     /gene="leuB"
                     /locus_tag="ACHLAG_001215"
     CDS             2003..3094
                     /gene="leuB"
                     /locus_tag="ACHLAG_001215"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFP0654482.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3097..4497
                     /gene="leuC"
                     /locus_tag="ACHLAG_001216"
     CDS             3097..4497
                     /gene="leuC"
                     /locus_tag="ACHLAG_001216"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFP0654483.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4508..5113
                     /gene="leuD"
                     /locus_tag="ACHLAG_001217"
     CDS             4508..5113
                     /gene="leuD"
                     /locus_tag="ACHLAG_001217"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFP0654484.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDNAIAAYEAKQPAFMN"
     gene            complement(5162..6340)
                     /gene="setA"
                     /locus_tag="ACHLAG_001218"
     CDS             complement(5162..6340)
                     /gene="setA"
                     /locus_tag="ACHLAG_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFP0654485.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6442..6573)
                     /gene="sgrT"
                     /locus_tag="ACHLAG_001219"
     CDS             complement(6442..6573)
                     /gene="sgrT"
                     /locus_tag="ACHLAG_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFP0654486.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6662..8317
                     /gene="sgrR"
                     /locus_tag="ACHLAG_001220"
     CDS             6662..8317
                     /gene="sgrR"
                     /locus_tag="ACHLAG_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFP0654487.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8351..8450
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8481..9464
                     /gene="thiB"
                     /locus_tag="ACHLAG_001221"
     CDS             8481..9464
                     /gene="thiB"
                     /locus_tag="ACHLAG_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFP0654488.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9440..11050
                     /gene="thiP"
                     /locus_tag="ACHLAG_001222"
     CDS             9440..11050
                     /gene="thiP"
                     /locus_tag="ACHLAG_001222"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFP0654489.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11034..11732
                     /gene="thiQ"
                     /locus_tag="ACHLAG_001223"
     CDS             11034..11732
                     /gene="thiQ"
                     /locus_tag="ACHLAG_001223"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFP0654490.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11846..12610)
                     /gene="yabI"
                     /locus_tag="ACHLAG_001224"
     CDS             complement(11846..12610)
                     /gene="yabI"
                     /locus_tag="ACHLAG_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP0654491.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12696..13574)
                     /gene="araC"
                     /locus_tag="ACHLAG_001225"
     CDS             complement(12696..13574)
                     /gene="araC"
                     /locus_tag="ACHLAG_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFP0654492.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13913..15613
                     /gene="araB"
                     /locus_tag="ACHLAG_001226"
     CDS             13913..15613
                     /gene="araB"
                     /locus_tag="ACHLAG_001226"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFP0654493.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDANGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15624..17126
                     /gene="araA"
                     /locus_tag="ACHLAG_001227"
     CDS             15624..17126
                     /gene="araA"
                     /locus_tag="ACHLAG_001227"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFP0654494.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17295..17990
                     /gene="araD"
                     /locus_tag="ACHLAG_001228"
     CDS             17295..17990
                     /gene="araD"
                     /locus_tag="ACHLAG_001228"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP0654495.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNISAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPAIGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18065..20416
                     /gene="polB"
                     /locus_tag="ACHLAG_001229"
     CDS             18065..20416
                     /gene="polB"
                     /locus_tag="ACHLAG_001229"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFP0654496.1"
                     /translation="MAQAGFILTRRWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHSQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20581..23487
                     /gene="rapA"
                     /locus_tag="ACHLAG_001230"
     CDS             20581..23487
                     /gene="rapA"
                     /locus_tag="ACHLAG_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFP0654497.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23499..24158
                     /gene="rluA"
                     /locus_tag="ACHLAG_001231"
     CDS             23499..24158
                     /gene="rluA"
                     /locus_tag="ACHLAG_001231"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFP0654498.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24275..25090)
                     /gene="djlA"
                     /locus_tag="ACHLAG_001232"
     CDS             complement(24275..25090)
                     /gene="djlA"
                     /locus_tag="ACHLAG_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFP0654499.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25345..27699
                     /gene="lptD"
                     /locus_tag="ACHLAG_001233"
     CDS             25345..27699
                     /gene="lptD"
                     /locus_tag="ACHLAG_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFP0654500.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27752..29038
                     /gene="surA"
                     /locus_tag="ACHLAG_001234"
     CDS             27752..29038
                     /gene="surA"
                     /locus_tag="ACHLAG_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFP0654501.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29038..30027
                     /gene="pdxA"
                     /locus_tag="ACHLAG_001235"
     CDS             29038..30027
                     /gene="pdxA"
                     /locus_tag="ACHLAG_001235"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFP0654502.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADTTLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLGVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            30024..30845
                     /gene="rsmA"
                     /locus_tag="ACHLAG_001236"
     CDS             30024..30845
                     /gene="rsmA"
                     /locus_tag="ACHLAG_001236"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFP0654503.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30848..31225
                     /gene="apaG"
                     /locus_tag="ACHLAG_001237"
     CDS             30848..31225
                     /gene="apaG"
                     /locus_tag="ACHLAG_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFP0654504.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31232..32080
                     /gene="apaH"
                     /locus_tag="ACHLAG_001238"
     CDS             31232..32080
                     /gene="apaH"
                     /locus_tag="ACHLAG_001238"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFP0654505.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            32105..32704
                     /locus_tag="ACHLAG_001239"
     CDS             32105..32704
                     /locus_tag="ACHLAG_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HFP0654506.1"
                     /translation="MADYFEIRADYNQHTITVYQAYNDAIADVAVRDGRFGASFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQHILAIRIKRTFFDTLLEQAVLTTPEAHVYPHAGI
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNLLINGKTTQAKSFLPPEKIYPVSAAARKALGMKNS"
     gene            32740..33207
                     /locus_tag="ACHLAG_001240"
     CDS             32740..33207
                     /locus_tag="ACHLAG_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654507.1"
                     /translation="MRQINSGLNTREIILAMIDEADEPPFAEQRTWHRRRIAFLCDCI
                     RVAIDYGFFCSVSQQSIMNITRMAEDVWITESKTTQLIDIWQQLKQLYPPETIPYVHK
                     LPTKELLELLLSSPDDLRIIEIPDIIGDVFDDQTWIRLFKKHFADIIQLRCDV"
     gene            complement(33306..33485)
                     /locus_tag="ACHLAG_001241"
     CDS             complement(33306..33485)
                     /locus_tag="ACHLAG_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFP0654508.1"
                     /translation="MNDKTFDFTSERDFVSRAHCVGLNLQATLQWNEVNEDAISALNE
                     FAEETGCFSDEYRKF"
     gene            complement(33506..33985)
                     /gene="folA"
                     /locus_tag="ACHLAG_001242"
     CDS             complement(33506..33985)
                     /gene="folA"
                     /locus_tag="ACHLAG_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFP0654509.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34178..36040)
                     /gene="kefC"
                     /locus_tag="ACHLAG_001243"
     CDS             complement(34178..36040)
                     /gene="kefC"
                     /locus_tag="ACHLAG_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFP0654510.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAVTPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36033..36563)
                     /gene="kefF"
                     /locus_tag="ACHLAG_001244"
     CDS             complement(36033..36563)
                     /gene="kefF"
                     /locus_tag="ACHLAG_001244"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFP0654511.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(36671..38002)
                     /gene="yaaU"
                     /locus_tag="ACHLAG_001245"
     CDS             complement(36671..38002)
                     /gene="yaaU"
                     /locus_tag="ACHLAG_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0654512.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38060..38347)
                     /gene="fixX"
                     /locus_tag="ACHLAG_001246"
     CDS             complement(38060..38347)
                     /gene="fixX"
                     /locus_tag="ACHLAG_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFP0654513.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38344..39630)
                     /gene="fixC"
                     /locus_tag="ACHLAG_001247"
     CDS             complement(38344..39630)
                     /gene="fixC"
                     /locus_tag="ACHLAG_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP0654514.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(39681..40622)
                     /gene="fixB"
                     /locus_tag="ACHLAG_001248"
     CDS             complement(39681..40622)
                     /gene="fixB"
                     /locus_tag="ACHLAG_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFP0654515.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(40637..41407)
                     /gene="fixA"
                     /locus_tag="ACHLAG_001249"
     CDS             complement(40637..41407)
                     /gene="fixA"
                     /locus_tag="ACHLAG_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFP0654516.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT        10022 a        11373 c        10838 g         9208 t
ORIGIN      
        1 aacgcgtgca ttttgtcccc tttttcctcg ttgattagat gcaaaaattt atgctgaaat
       61 atgtcaaccg atgaaaagcg tcggtagtta agcagaaatt aatatcgctt actttaacca
      121 ccgcagcaca attagctaat tttacggatg cagaactcac gctggcggga cgtttttatt
      181 gcgtcagggt tgacatccgt ttttgtatcc agtaactcta aaagcatatc gcattcatct
      241 ggagctgatt taatgactca catcgttcgc tttatcggtc tactactact aaacgcatct
      301 tctttgcgcg gtagacgagt gagcggcatc cagcattaag ccagcacgca gtcaaacaaa
      361 aaacccgcgc cattgcgcgg gtttttttat gcccgaagcg aggcgctcta aaagagacaa
      421 ggacccaaac catgagccag caagtcatta ttttcgatac cacattgcgc gacggtgaac
      481 aggcgttaca ggcaagcttg agtgtgaaag aaaaactgca aattgcgctg gcccttgagc
      541 gtatgggtgt tgacgtgatg gaagtcggtt tccccgtctc ttcgccgggc gattttgaat
      601 cggtgcaaac catcgcccgc caggttaaaa acagccgcgt atgtgcgtta gctcgctgcg
      661 tggaaaaaga tatcgacgtg gcggccgaat ccctgaaagt cgccgaagcc ttccgtattc
      721 atacctttat tgctacctcc ccaatgcaca tcgccaccaa gctgcgcagc accctggacg
      781 aggtgatcga acgcgctatc tatatggtga aacgcgcccg taattacacc gatgatgttg
      841 aattttcttg cgaagatgcc gggcgtacac ccattgccga tctggcgcga gtggtcgaag
      901 cggcgattaa tgccggtgcc accaccatca acattccgga caccgtgggc tacaccatgc
      961 cgtttgagtt cgccggaatc atcagcggcc tgtatgaacg cgtacctaac atcgacaaag
     1021 ccattatttc cgtacatacc cacgacgatt taggcctggc ggtcggcaac tcactggcgg
     1081 cggtacatgc cggtgcacgc caggtggaag gtgcaatgaa cgggatcggc gagcgtgccg
     1141 gtaactgttc gctggaagaa gtcatcatgg cgattaaagt tcgtaaggat attctcaacg
     1201 tccacaccgc cattaatcac caggagatat ggcgcaccag ccagttagtt agccagattt
     1261 gtaatatgcc gatcccggca aacaaagcca ttgttggcag cggcgcattc gctcactcct
     1321 ccggtatcca ccaggatggc gtgctgaaaa accgcgaaaa ctacgaaatc atgacaccag
     1381 aatctattgg tctgaaccaa atccagctga atctgacctc tcgttcgggg cgtgcggcgg
     1441 tgaaacatcg catggatgag atggggtata aagaaagtga atataattta gacaatttgt
     1501 acgatgcttt cctgaagctg gcggacaaaa aaggtcaggt gtttgattac gatctggagg
     1561 cgctggcctt catcggtaag cagcaagaag agccggagca tttccgtctg gattacttca
     1621 gcgtgcagtc tggctctaac gatatcgcca ccgccgccgt caaactggcc tgtggcgaag
     1681 aagtcaaagc agaagccgcc aacggtaacg gtccggtcga tgccgtctat caggcaatta
     1741 accgcatcac tgactataac gtcgaactgg tgaaatacag cctgaccgcc aaaggtcacg
     1801 gtaaagatgc gctgggtcag gtggatattg tcgccaacta caacggtcgc cgcttccacg
     1861 gcgtcggcct ggccaccgat attgtcgagt cctccgccaa agccatggtg cacgtactga
     1921 acaatatctg gcgtgccgca gaagtcgaaa aagagttgca acgcaaagct caacacaacg
     1981 aaaacaacaa ggaaaccgtg tgatgtcgaa gaattaccat attgccgtat tgccggggga
     2041 cggtattggt ccggaagtga tgacccaggc gctgaaagtg ctggatgccg tgcgcaaccg
     2101 ctttgcgatg cgcatcacca ccagccatta cgatgtaggc ggcgcagcca ttgataacca
     2161 cgggcaacca ctgccgcctg cgacggttga aggttgtgag caagccgatg ccgtgctgtt
     2221 tggctcggta ggcggtccga aatgggaaca tttaccacca gaccagcaac cagaacgcgg
     2281 cgcgctgttg cctttgcgta agcacttcaa attattcagc aacctgcgcc cggcaaaact
     2341 gtatcagggg ctggaagcat tctgtccgct gcgtgcagac attgccgcta acggcttcga
     2401 catcctgtgc gtgcgcgaac tgaccggcgg catctatttc ggtcagccaa aaggccgcga
     2461 aggtagcgga caatatgaaa aagcgtttga taccgaggtg tatcaccgtt ttgagatcga
     2521 gcgtattgcc cgcatcgcgt ttgaatctgc tcgtaaacgt cgccacaaag tcacctcaat
     2581 cgacaaagct aacgtgctgc aatcctctat tttatggcgt gaaatcgtca acgagatcgc
     2641 cacggaatac ccggatgtcg aactggcgca tatgtacatc gacaacgcca ccatgcagct
     2701 gattaaagat ccatcacagt ttgacgttct gctgtgctcc aacctgtttg gcgacattct
     2761 gtctgacgag tgcgcaatga tcactggctc gatggggatg ttgccttccg ccagcctgaa
     2821 cgagcaaggt tttggtctgt atgaaccggc aggcggctca gcaccagata tcgcaggcaa
     2881 aaacatcgcc aacccgattg cgcaaattct gtcgctggca ctgctgctgc gctacagcct
     2941 ggatgccgat gatgcggctt gcgccattga acgcgccatt aaccgcgcat tagaagaagg
     3001 cattcgcacc ggggatttag cccgtggcgc tgccgccgtt agtaccgatg aaatgggcga
     3061 tatcattgcc cgctatgtgg cagaaggggt gtaatcatgg ctaaaacgtt atacgaaaaa
     3121 ttattcgacg ctcacgtagt gtacgaagcc gaaaacgaaa ccccgctgtt atatatcgac
     3181 cgccacctgg tgcatgaagt gacctcaccg caggcgtttg atggtctgcg cgcccacggt
     3241 cgcccggtac gtcagccggg caaaaccttc gccactatgg atcacaacgt ctctacccag
     3301 accaaagaca ttaatgcctg tggtgaaatg gcgcgcatcc agatgcagga gctgattaaa
     3361 aactgcaaag aatttggcgt cgagctgtat gacctgaatc acccgtatca ggggatcgtc
     3421 cacgtaatgg ggccggaaca gggcgtcacc ttgccgggga tgaccattgt ctgcggcgac
     3481 tcgcataccg ccacccacgg cgcgtttggc gcactggcct ttggtatcgg cacttccgaa
     3541 gttgaacacg tactggcaac acaaaccctg aaacagggcc gtgcgaagac catgaaaatt
     3601 gaagtccagg gcaaagccgc gccgggcatt acagcaaaag atatcgtgct ggcaattatc
     3661 ggtaaaactg gcagcgcagg cggcaccggg catgtggtgg agttttgcgg cgaagcaatt
     3721 cgtgatttaa gcatggaagg tcgtatgacc ctgtgcaata tggcaatcga aatgggcgca
     3781 aaagccggtc tggttgcacc ggacgaaacc acctttaact atgtcaaagg ccgtttgcat
     3841 gcgccgaaag gcaaagattt cgacgacgcc gttgcctact ggaaaaccct gcaaaccgac
     3901 gaaggcgcaa ctttcgatac cgttgtcact ctgcaagcag aagaaatttc accgcaggtc
     3961 acctggggca ccaatcctgg ccaggtgatt tccgtgaacg acaatattcc cgatccggct
     4021 tcgtttgccg atccggttga acgcgcgtcg gcagaaaaag cgctggccta tatggggctg
     4081 aaaccgggca ttccgctgac cgaagtggct atcgacaaag tgtttatcgg ttcctgtacc
     4141 aactcgcgca ttgaagattt acgcgcggca gcggagatcg ccaaagggcg aaaagtcgcg
     4201 ccaggcgtgc aggcactggt ggttcccggc tctggcccgg taaaagccca ggcggaagcg
     4261 gaaggtctgg ataaaatctt tattgaagcc ggttttgaat ggcgcttgcc tggctgctca
     4321 atgtgtctgg cgatgaacaa cgaccgtctg aatccgggcg aacgttgtgc atccaccagc
     4381 aaccgtaact ttgaaggccg ccaggggcgc ggcgggcgca cgcatctggt cagcccggca
     4441 atggctgccg ctgctgctgt gaccggacat ttcgccgaca ttcgcaacat taaataagga
     4501 gcacaccatg gcagagaaat ttatcaaaca cacaggcctg gtggttccgc tggatgccgc
     4561 caatgtcgat accgatgcaa tcatcccgaa acagtttttg cagaaagtga cccgtacggg
     4621 ttttggcgcg catctgttta acgactggcg ttttctcgat gaaaaaggcc aacagccaaa
     4681 cccggacttc gtgctgaact tcccgcagta tcagggcgct tccattttgc tggcacgaga
     4741 aaacttcggc tgtggctcct cgcgtgagca cgcgccctgg gcattgaccg actacggttt
     4801 taaagtggtg attgcgccga gttttgctga catcttctac ggcaatagct ttaacaacca
     4861 gctgctgccg gtgaaattaa gcgatgcaga agtggacgaa ctgtttgcgc tggtgaaagc
     4921 caatccgggg atccatttcg acgtggatct ggaagcgcaa gaggtgaaag cgggagagaa
     4981 aacctatcgc tttaccatcg atgccttccg ccgccactgc atgatgaacg gtctggacag
     5041 tattgggctt accttgcagc acgacaacgc cattgccgct tatgaagcaa aacaacctgc
     5101 gtttatgaat taatcccctt gcccggtcaa atgaccgggc tttccgctat cgtccacgtc
     5161 atcaaacgtc tttaaccttt gcggttaaaa ataatgcgac aacagaaata accgcaatta
     5221 cccagtagac agcaaagtgc ccccaacttt gtgcaattgc tccctgaata acgccagcca
     5281 gaattacccc ggtagaaata ctgttagtaa ataaggtggt agctgccccc gctcttccag
     5341 gcattaaatc ctgaaaccat agcatcccaa tacccgcaac aatgccgata aatacagcgt
     5401 taaaaagttg cagcgtcatc aacgccatac ggctatgaaa gaaaatcaat ccggtgtaaa
     5461 acagtactcc tgccgccact gctatgacca tcattcgccg cttaccataa cgtttgacat
     5521 agtagccagc cagaatcatt gctggtattt ccagtccagc tgccgtcccc atcaggaaac
     5581 ccgccagttt gtctggcaat cctaactcgc tactgatcca caacggcata tcaataatgt
     5641 acatggtgtt gcaggtccac attaacgtcg aggcgacaaa taacatccgt acgttactat
     5701 cctgccagcc accttgcatt gataaagcat tttccgacgg cagttctacc cgcgccacag
     5761 acggaagcat aaatgcaatc aataccagac tgagtgtgaa tatcccggcg gcaatcgaaa
     5821 acatcaccgt aaagccgtaa ttcaacgcca gcataaaggc caacggtgga ccgataaccc
     5881 atgccagaga aagctgcgca cgcatcaccg agctaaacat caccacttct cgcgccgagt
     5941 tatccgcata ttcccgcgcc agagcaaata actgtggcat tgccgtattg gccagagatg
     6001 ccagaagcac accacaggtg ataagcgtca gataatgacg attaaatgca aacaatagcg
     6061 cattgccgat agccatcaaa cagcaaaata taatcagttt tcgccgatcg ccctgactgt
     6121 cagaacgttt tgccaaccag aggcttacgc cgatcccagc aatagcattc accgtataaa
     6181 agagtccgat ccagaaaggt tgcgcgccaa cctcacgact cagaaataag ctcaatgtag
     6241 gagcctgtag cgccccggcc acccccatca taaaagcgac cagcataaat gccgcgtaaa
     6301 caccgttcat acggcgagcc atcgtcatta tccagatcat acgttccctt tttagcgcgg
     6361 cgagaataaa aaaaaccagc aggtataatc tgctggcggg tgattttaca ccaatactca
     6421 gtcacacatg atgcaggcaa gtcaactttc agaattgcgg tcatcccact gcatcagtcc
     6481 ttcaagcatg gttaatcgtt gtgggacgct taaccaacgc aaccagcaca acttcgctgt
     6541 cgcggtaaaa tagtgctgat aaaactgacg catggggcac ctccttgctt catcgttagg
     6601 aattattgac ttaatatagg gaaaataaaa ttgctgtctt ttgcgcagga gttccccttt
     6661 tatgccatct gctcgtctgc aacaacagtt catccgcctg tggcaatgct gcgagggtaa
     6721 atcgcaggac acaacgctca acgaactggc agcgttattg agctgctcgc gtcgtcatat
     6781 gcgcaccctg ctcaacacca tgcaggatcg cggctggctg acgtgggaag cggaagtcgg
     6841 gcgtggtaaa cgctcgcgtc tgacattcct ctataccggg ctggcgcttc agcaacagcg
     6901 ggcggaagac ctgctggagc aggatcgtat cgaccaacta gtgcagttgg ttggcgacaa
     6961 agcgactgtg cggcaaatgc tggtttctca tctgggccgc agcttccgcc aggggcggca
     7021 catcctgcgc gtgctctact atcgtccgtt gcgtaatctg ctacctggca gcgcattgcg
     7081 ccgttccgaa acccatattg cccggcaaat cttcagttcg ctaacgcgca taaatgagga
     7141 aaatggggaa ctggaagcag acatcgccca ccactggcag caaatatcac cgcttcactg
     7201 gcgtttcttt ttgcgtccag gagtccattt tcatcatggt cgtgaactgg aaatggacga
     7261 cgtgatcgcc tctttaaaac gaatcaatac gctgccgctc tattcgcata ttgcggacat
     7321 tgtgtcgccg acgccctgga cgctggatat ccatctcacg caaccggacc gctggttacc
     7381 gttactgctg ggacaagttc cggcgatgat cctgccgcgc gaatgggaaa ccctcagtaa
     7441 ctttgccagc catcccatcg gcaccggtcc gtatgcggtg atccgcaaca ccaccaatca
     7501 actgaaaatt caggcattcg atgacttctt cggttaccgg gcattaatcg atgaagttaa
     7561 cgtctgggtt ctgccggaaa ttgccgacga gccagctgga ggtctgatgc taaaaggtcc
     7621 acagggtgag gaaaaagaga ttgaaagccg cctggaggaa ggttgctact atttactgtt
     7681 cgacagccgc acccatcgcg gggcgaatca gcaagtcagg gactgggtaa gctatgtgct
     7741 ttctccaact aatctggtct atttcgctga ggaacagtac cagcaactgt ggttcccggc
     7801 ttatggactg ctcccccgtt ggcaccatgc ccgcaccata aagagcgaaa aaccggctgg
     7861 cctggaaagc ctcaccctga ccttttatca ggatcacagt gagcatcggg tgattgccgg
     7921 gatcatgcag cagattctgg caagtcacca ggtcacgctg gaaatcaaag agatcagcta
     7981 cgatcagtgg catgaaggag agatcgagag cgatatctgg cttaacagcg ccaactttac
     8041 gctgccgctg gatttttcgc tgttcgcgca cttgtgcgag gtgccgctgc tccaacactg
     8101 tattccgatc gactggcaag ccgacgccgc ccgctggcgc aatggcgaaa tgaacctggc
     8161 gaactggtgc cagcaactgg tcgccagcaa agcaatggtg ccgcttatcc accactggct
     8221 gatcattcag gggcaacgca gtatgcgcgg cctgcgcatg aacaccctcg gctggttcga
     8281 ttttaaatca gcgtggtttg cgccaccgga tccatgattg ctggtagata acaaaatcac
     8341 tacactaacg ccgttctcaa cggggtgcca cgcaaacgcg tgcgctgaga aaatacccgt
     8401 cgaacctgat ccggataacg ccggcgaagg gatttgaggc tccttctcaa gtcctttgcc
     8461 actctttttt gaggtgcaaa gtgttaaaaa aatgtcttcc cctgctgttg ctgtgcacag
     8521 cgcccgtttt cgctaaaccc gttctgactg tttataccta cgattccttc gccgccgact
     8581 gggggcctgg tccaaagatc aaaaaagctt ttgaagccga ctgtaactgc gaactgaaac
     8641 tggtggcgct ggaagatggc gtttcgcttc tcaaccgtct acggatggaa ggcaaaaata
     8701 gtaaagccga tgtggtgctg gggctggata acaatctgtt agacgccgcc agtaaaaccg
     8761 gactatttgc caaaagcggt gtggcagcgg atgccgttaa cgttcccggc ggctggaata
     8821 atgacacttt cgtcccgttt gattacggct atttcgcctt cgtctatgac aagaacaaac
     8881 tgaaaaaccc gccgcaaagc ctgaaagaat tggttgagag cgatcagaac tggcgggtga
     8941 tttatcagga tccgcgcacc agtacgccgg ggctgggcct gctgttgtgg atgcaaaaag
     9001 tctatggcga tgacgcccca caagcctggc aaaaactggc gaagaaaacc gtcacggtca
     9061 ccaaaggctg gagcgaagcc tacggcctgt ttttaaaagg tgaaagcgat ctggtactga
     9121 gttacaccac ctctccggct tatcacattc tcgaagagaa aaaagataac tacgccgccg
     9181 cgaactttag cgaaggtcac tatctgcagg tggaagtcgc cgcccgcacc gctgccagca
     9241 agcagccgga gctggcgcaa aaattcctcc agtttatggt ttctccggct tttcagaatg
     9301 cgatcccaac cggtaactgg atgtatccgg tggcaaatgt cacgctgcct gccgggtttg
     9361 aacaattgac caaaccggcc accacgctgg agttcacacc agctgaagtg gcggcacaac
     9421 gtcaggcatg gattagcgaa tggcaacgcg ccgtcagccg ttaattcccg gctggttaat
     9481 tccaggtgta agcgccgcca cgctggtggt agcggttgcg ctcgcggcgt ttctcgccct
     9541 gtggtggaac gcgccgcagg gtaactgggt ggcagtctgg caggacagct acctgtggca
     9601 tgtggtgcgc ttctccttct ggcaggcgtt tctctcggcg ctactctctg tcgtacccgc
     9661 gatattcctc gcccgcgccc tctatcgcag gcgctttccg ggtcggctgg cgctgttgcg
     9721 tctgtgcgca atgaccttga tcctcccggt gctagttgct gttttcggca ttcttagcgt
     9781 ctatggtcgt cagggctggc tggcatcgct ctgccaatcg ctcggtctgg agtggacctt
     9841 ttcgccctac ggcctgcaag gcattttgct ggcgcacgta ttttttaatc tgccgatggc
     9901 gagccgctta ttactccagg cactggaaaa cattcccggc gaacaacgtc agcttgccgc
     9961 ccagcttggg atgcgcggct ggcatttttt ccgcttcgtc gaatggccgt ggctacggcg
    10021 acaaatcccg ccggttgccg cgcttatctt tatgctctgt ttcgccagct tcgccaccgt
    10081 gctatcgctg ggcggcggtc cgcaggcaac tactatcgag ctggctattt atcaggcgct
    10141 gagttacgac tacgatcctg cccgcgcggc gatgctggcg ctgctccaga tggtgtgttg
    10201 ccttgggctg gtgctgttga gtcagcgact aagtaaggcc attgcgcccg gtaccacgct
    10261 gctgcaaggc tggcgcgatc cggacgatcg tctgcatagc cgcatttgcg acacggtgtt
    10321 aatagtgctg gcgctgctgc tgttgctgcc accgttgctg gcggtgatcg tcgatggggt
    10381 aaatcgccag ttgccggaag tgctggcaca accggtgctg tggcaggcgc tgtggacctc
    10441 gttgcgtatt gcgctggcgg caggtgtatt gtgcgtagtg ctgaccatga tgctgctatg
    10501 gagcagtcgc gaacttcggg cgcggcagaa aatgctggcg ggtcaggcgc tggagatgag
    10561 cggcatgttg atcctcgcca tgccggggat tgtgctggct accggcttct ttttactgct
    10621 caacaacacc attggcctgc cgcaatctgc tgacggcatt gtgattttca ccaatgcgtt
    10681 aatggcgatc ccttatgcgc tgaaagtact ggaaaacccg atgcgcgata tcaccgcccg
    10741 ctacagtatg ttgtgtcagt cgctgggcat tgaaggctgg tcgcgcttaa aagtggtcga
    10801 gctgcgcgcc ctgaaacgtc cactggcgca ggcgctggct tttgcctgcg tgctgtcgat
    10861 tggtgatttt ggcgtagtgg cgttgttcgg taacgatgat ttccgtaccc tgccgtttta
    10921 tctctaccag caaattggct cctatcgcag ccaggacggt gcggtcaccg cgttaattct
    10981 gctactactc tgttttctgc tgtttaccgt gattgaaaaa ctaccggggc gaaatgttaa
    11041 aactgactga tatcacctgg ctttatcacc atttgccgat gcgttttagc ttaacggtag
    11101 aacgcggcga gcaggtggca atcctcgggc caagcggcgc gggtaaaagt accctgctga
    11161 atttgatcgc cggttttctg acgccagcca gcggttcgct gactatcgac ggcgttgatc
    11221 acaccacgac gccgccgtca cgccgtccgg tgtcgatgct gtttcaggaa aacaacctgt
    11281 tcagtcacct gacggtcgcg cagaacatcg ggctggggct aaatccggga ttgaaactga
    11341 acgcggcaca gcaggagaaa atgcacgcta tcgcccgcca gatggggatt gataatttaa
    11401 tggcgcggtt acctggcgag ctttccggcg gtcagcgaca gcgcgtggcg ttagcgcgtt
    11461 gtctggtgcg cgaacagccg attttattgc tcgatgaacc gttctctgcg ctcgatccgg
    11521 cgttacgtca ggagatgctg acgctggtga gcacgagctg ccagcagcaa aaaatgacgc
    11581 tattgatggt gtcgcacagt gtggaagatg cggcgcggat cgccacacgc tcggtggtgg
    11641 tcgccgacgg gcgcatcgcc tggcagggta agaccaatga gttgttgagc ggtaaggcga
    11701 gtgcttcggc actattgggg attacgggtt agtatgcact gtcggatgcg gcgtgaacgc
    11761 cttatccgac ctacgggtag tgcgttttgt aggcctgata agacgcggtc agcgtcgcat
    11821 caggcaaccc gcacaagact atctcctaac ccccaaccac tttacgcaaa atatcgatat
    11881 acaccggcat caacgggtgg cgaattaaca ccaccagcgc caccacgccg atggcagaaa
    11941 ttaacggcgt cagccacaac aaacgaccac gggacaaata atgactcaaa cggtcagtcg
    12001 ctttaccact gcgccataac cgccagcaca gccagccacc aacccacaaa aacaccgctg
    12061 ttgccagcag caaccattta aactcaccgc tctgcattcc ggctggaata tcgatcgccg
    12121 cgcccgccag aatccctggc aggaagtaaa acggtggcca cagcaggcag ccgataatat
    12181 tcggcgtaat gaattttgcc accggcagat ccagcattcc cgccaccatt ggcaccagcg
    12241 gacgcgtcgg gccaacaaaa cgaccgacca gaatggtgaa catgctgtgt tgatgcaacg
    12301 catgttcagt tttatcgagt aatgctttgt ttttcttcag aaatgaccag cgatgcaacg
    12361 gctttttaaa acgccagccc agccagaagg agatccagtc gcccagcagg caaccgacga
    12421 tccctgccag ccaggcgtgc cagaaactta actcgccgct gccaatcagc gctcccagcc
    12481 ccgccatcag caccgtaccg ggtagaatca aaccgaccag cgccagcgac tccagaaagg
    12541 ccaccaacac caccgccatc aatgaataca cggtagattg ggtaataaag tgttccagca
    12601 atgcttgcat aatgtgcctg tcaaatggac gaagcaggga ttctgcaaac cctatgctac
    12661 tccgtcaagc cgtcaattgt ctgattcgtt accaattatg acaacttgac ggctacatca
    12721 ttcacttttt cttcacaacc ggctcggaac tcgctcgggc tggccccggt gcatttttta
    12781 aatacccgcg agaaatagag ttgatcgtca aaaccaacat tgcgaccgac ggtggcgata
    12841 ggcatccggg tggtgctcaa aagcagcttc gcctggctga tacgttggtc ctcgcgccag
    12901 cttaagacgc taatccctaa ctgctggcgg aaaagatgtg acagacgcga cggtgacaag
    12961 caaacatgct gtgcgacgct ggcgatatca aaattgctgt ctgccaggtg atcgctgatg
    13021 tactgacaag cctcgcgtac ccgattatcc atcggtggat ggagcgactc gttaatcgct
    13081 tccatgcgcc gcagtaacaa ttgctcaagc agatttatcg ccagcagctc cgaatagcgc
    13141 ccttcccctt gcccggcgtt aatgatttgc ccaaacaggt cgctgaaatg cggctggtgc
    13201 gcttcatccg ggcgaaagaa acccgtattg gcaaatattg acggccagtt aagccattca
    13261 tgccagtagg cgcgcggacg aaagtaaacc cactggtgat accattcgcg agcctccgga
    13321 tgacgaccgt agtgatgaat ctctcctggc gggaacagca aaatatcacc cggtcggcat
    13381 acaaattctc gtccctgatt tttcaccacc ccctgaccgc gaatggtgag attgagaata
    13441 taacctttca ttcccagcgg tcggtcgata aaaaaatcga gataaccgtt ggcctcaatc
    13501 ggcgttaaac ccgccaccag atgggcatta aacgagtatc ccggcagcag gggatcattt
    13561 tgcgcttcag ccatactttt catactcccg ccattcagag aagaaaccaa ttgtccatat
    13621 tgcatcagac attgccgtca ctgcgtcttt tactggctct tctcgctaac ccaaccggta
    13681 accccgctta ttaaaagcat tctgtaacaa agcgggacca aagccatgac aaaaacgcgt
    13741 aacaaaagtg tctataatca cggcagaaaa gtccacattg attatttgca cggcgtcaca
    13801 ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg acgcttttta
    13861 tcgcaactct ctactgtttc tccatacccg tttttttgga tggagtgaaa cgatggcgat
    13921 tgcaattggc ctcgattttg gcagtgattc tgtgcgagct ttggcggtgg actgcgccag
    13981 cggtgaagag atcgccacca gcgtagagtg gtatccccgt tggcaaaaag ggcaattttg
    14041 tgatgccccg aataaccagt tccgtcatca tccgcgtgac tacattgagt caatggaagc
    14101 ggcactgaaa accgtgcttg cagagcttag cgtcgaacag cgcgcagctg tggtcgggat
    14161 tggcgttgac acaaccggct cgacgcccgc accgattgat gccaacggta acgtgctggc
    14221 gctgcgcccg gagtttgccg aaaacccgaa cgcgatgttc gtattgtgga aagaccacac
    14281 tgcggttgaa gaagcggaag agattacccg tttgtgccac gcgccgggca acgttgacta
    14341 ctcccgctac attggcggta tttattccag cgaatggttc tgggcaaaaa tcctgcatgt
    14401 gactcgccag gacaccgccg tggcgcaatc tgccgcatcg tggattgagc tgtgcgactg
    14461 ggtgccagct ctgctttccg gtaccacccg cccgcaggat attcgtcgcg gacgttgcag
    14521 cgccgggcat aagtcgctat ggcacgaaag ctggggtggc ttgccgccag ccagtttctt
    14581 tgatgagcta gacccgatcc tcaatcgcca tttgccttcc ccgctgttca ctgacacctg
    14641 gactgccgat attccggtgg gcaccttatg cccggaatgg gcgcagcgtc tcggcctgcc
    14701 tgaaagcgtg gtgatttccg gcggcgcgtt tgactgccat atgggcgcag ttggcgcagg
    14761 cgcacagcct aacgcactgg taaaagttat cggtacttcc acctgcgaca ttctgattgc
    14821 cgacaaacag agcgttggcg agcgggcagt gaaaggtatt tgcggtcagg ttgatggcag
    14881 cgtggtacct ggatttatcg gtctggaagc aggccaatcg gcgtttggtg atatctacgc
    14941 ctggtttggt cgcgtactcg gctggccgct ggaacagctt gccgcccagc atccggaact
    15001 gaaagcgcaa atcaacgcca gccagaaaca actgcttccg gcgctgaccg aagcatgggc
    15061 caaaaatccg tctctggatc acctgccggt ggtgctcgac tggtttaacg gccgccgcac
    15121 accgaacgct aaccaacgcc tgaaaggggt gattaccgat cttaacctcg ctaccgacgc
    15181 tccgctgctg ttcggcggtt tgattgctgc caccgccttt ggcgcacgcg caatcatgga
    15241 gtgctttacc gatcagggga tcgccgtcaa taacgtgatg gcgctgggcg gcatcgcgcg
    15301 gaaaaaccag gtcattatgc aggcctgctg cgacgtgctg aatcgcccgc tgcaaattgt
    15361 tgcctctgac cagtgctgtg cgctcggtgc ggcgattttt gctgccgtcg ccgcgaaagt
    15421 gcacgcagac atcccatcag cccagcaaaa aatggccagt gcggtagaga aaaccctgca
    15481 accgcgcagc gaacaggcac aacgctttga acagctttat cgccgctatc agcaatgggc
    15541 gatgagcgcc gaacaacact atcttccaac ttccgccccg gcacaggctg cccaggccgt
    15601 tgcgactcta taaggacacg ataatgacga tttttgataa ttatgaagtg tggtttgtca
    15661 ttggcagcca gcatctgtat ggcccggaaa ccctgcgtca ggtcacccaa catgccgagc
    15721 acgttgttaa tgcgctgaat acggaagcga aactgccctg caaactagtg ttgaaaccgc
    15781 tgggcaccac gccggatgaa atcaccgcta tttgccgcga cgcgaattac gacgatcgtt
    15841 gcgctggtct ggtggtgtgg ctgcacactt tctccccggc caaaatgtgg atcaacggcc
    15901 tgaccatgct caacaaaccg ttgctgcaat tccacaccca gtttaacgcg gcgctgccgt
    15961 gggacagtat cgatatggac tttatgaacc tgaaccagac tgcacatggc ggtcgcgagt
    16021 tcggcttcat tggcgcgcgt atgcgtcagc aacatgccgt ggttaccggt cactggcagg
    16081 ataaacaagc ccatgagcgt atcggctcct ggatgcgcca ggcggtctct aaacaggata
    16141 cccgtcatct gaaagtctgc cgttttggcg ataacatgcg tgaagtagcg gtcaccgatg
    16201 gtgataaagt tgccgcacag atcaagttcg gtttctccgt caatacctgg gcggttggcg
    16261 atctggtgca ggtggtgaac tccatcagcg acggcgatgt taacgcgctg gtcgatgagt
    16321 acgaaagctg ctacaccatg acgcctgcca cacaaatcca cggcgaaaaa cgacagaacg
    16381 tgctggaagc ggcgcgtatt gagctgggga tgaagcgttt cctggaacaa ggtggcttcc
    16441 acgcgttcac caccaccttt gaagatttgc acggtctgaa acagcttcca ggtctggccg
    16501 tacagcgtct gatgcagcag ggttacggct ttgcgggcga aggcgactgg aaaaccgccg
    16561 ccctgcttcg catcatgaag gtgatgtcaa ccggtctgca gggcggcacc tcctttatgg
    16621 aggactacac ctatcacttc gagaaaggta atgacctggt gctcggctcc catatgctgg
    16681 aagtctgccc gtcgattgcc gtagaagaga aaccgatcct cgacgttcag catctcggta
    16741 ttggtggtaa ggacgatcct gcccgactga tcttcaatac ccaaaccggt ccagcgattg
    16801 tcgccagcct gattgatctc ggcgatcgtt accgtctgct ggttaactgt atcgacacgg
    16861 tgaaaacacc gcactccctg ccgaaactgc cggtggcgaa tgcgctgtgg aaagcgcaac
    16921 cggatctgcc aactgcttcc gaagcgtgga tccttgctgg tggcgcgcac cataccgtct
    16981 tcagccatgc gctgaacctc aacgatatgc gccagttcgc cgagatgcac gacattgaaa
    17041 tcacggtgat tgataacgac acccgcctgc cagcgtttaa agacgcactg cgctggaacg
    17101 aagtgtatta cggatttcgt cgctaagtag ccacatcagg tatgtaacgc ctgatgcgac
    17161 gctgacgcgt cttatctggc ctacacgctg cgattttgta ggccggataa gcaaagcgca
    17221 tccggcattg ttttccccct cgcccctttg gggagagggc cggggtgagg ggaaacgacc
    17281 tctcaggagt caacatgtta gaagatctca aacgccaggt attagaagcc aacctggcgc
    17341 tgccaaaaca caacctggtc acgctcacat ggggcaacat cagcgccgtt gatcgcgagc
    17401 gcggcgtctt tgtgatcaaa ccttccggcg tcgattacag cgtcatgacc gctgacgata
    17461 tggtcgtggt tagcatcgaa accggtgaag tggttgaagg tacgaaaaag ccctcctccg
    17521 acacgccaac tcaccggctg ctctatcagg cattcccctc cattggcggc attgtgcata
    17581 cgcactcgcg ccacgccacc atctgggcgc agtcgggtca gtcgattcca gcaatcggca
    17641 ccacccacgc cgactatttc tacggcacca ttccctgcac ccgcaaaatg accgacgcag
    17701 aaatcaacgg cgaatatgag tgggaaaccg gtaacgtcat cgtagaaacc tttgaaaaac
    17761 agggtatcga tgcagcgcaa atgcccggcg ttctggtcca ttcccacggc ccgtttgcat
    17821 ggggcaaaaa tgccgaagat gcggtgcata acgccatcgt gctggaagag gtcgcttata
    17881 tggggatatt ctgccgtcag ttagcgccgc agttaccgga tatgcagcaa acgctgctgg
    17941 ataaacacta tctgcgtaag catggcgcga aggcatatta cgggcagtaa tgactgtata
    18001 aaaccacagc caatcaaacg aaaccaggct atactcaagc ctggtttttt gatggatttt
    18061 cagcgtggcg caggcaggtt ttatcttaac ccgacgctgg cgggacaccc cgcaagggac
    18121 agaagtctcc ttctggctgg cgacggacaa cgggccgttg caggttacgc ttgcaccgca
    18181 agaatccgtg gcgtttattc ccgccgatca ggttccccgc gctcagcata ttttgcaggg
    18241 tgaacaaggc tttcgcctga caccgctggc gttaaaggat tttcaccgcc agccggtgta
    18301 tggcctttac tgtcgcgccc atagccaatt gatgaattac gaaaagcgcc tgcgtgaagg
    18361 tggcgttacc gtctacgagg ccgatgtgcg cccgccagaa cgctatctga tggagcggtt
    18421 tatcacctca ccggtgtggg tcgagggtga tatgcacaat ggcactatcg ttaatgcccg
    18481 tctgaaaccg catcccgact atcgtccgcc gctcaagtgg gtttctatag atattgaaac
    18541 cacccgccac ggtgagctgt actgcatcgg cctggaaggc tgcgggcagc gcatcgttta
    18601 tatgctgggg ccggagaatg gcgacgcctc ctcgcttgat ttcgaactgg aatacgtcgc
    18661 cagccgcccg ctgctgctgg aaaaactcaa cgcctggttt gcaaaccacg atcccgatgt
    18721 gatcatcggc tggaacgtgg tgcagttcga tctgcgaatg ctgcaaaaac atgccgagcg
    18781 ttaccgtctt ccgctgcgtc ttgggcgcga taatagcgag ctggagtggc gcgagcacgg
    18841 ctttaaaaac ggcgtctttt ttgcccaggc taaaggtcgg ctaattatcg acggtatcga
    18901 ggcgctgaaa tccgcgttct ggaatttctc ttcattctcg ctggaaactg tcgctcagga
    18961 gctattaggc gaaggaaaat ctatcgataa cccgtgggat cgaatggacg aaattgaccg
    19021 ccgtttcgcc gaagataaac ctgcgctggc aacttataac ctgaaagatt gcgagctggt
    19081 gacgcagatc ttccacaaaa ctgaaatcat gccattttta ctcgaacgag caacggtgaa
    19141 cggcctgccg gtggatcggc acggcggttc ggtggcagcg tttggtcatc tctattttcc
    19201 gcgaatgcat cgcgctggtt atgtcgcgcc taatctcggc gaagtgccac cgcacgccag
    19261 ccctggcggc tacgtgatgg attcacggcc agggctttat gattcagtgc tggtgctgga
    19321 ctataaaagc ctgtacccgt cgatcatccg cacctttctg attgatcccg tcgggctggt
    19381 tgaaggcatg gcgcaacctg atccagagca cagtaccgaa ggttttctcg atgcctggtt
    19441 ctcacgggag aaacattgcc tgccggagat tgtgactaac atctggcacg ggcgcgatga
    19501 agccaaacgt cagggtaata agccgctttc gcaggcgctg aagatcatta tgaatgcctt
    19561 ttatggcgtg ctcggcacca ccgcctgccg cttcttcgat ccgcgcctgg catcgtcgat
    19621 caccatgcgt ggtcatcaga tcatgcggca gaccaaagca ttgattgaag cgcagggcta
    19681 cgacgttatc tacggagata ccgactcgac ttttgtctgg ctaaaaggtg cacattcgga
    19741 agaagaggcg gcgaaaatcg gtcgtgcact ggtgcagcac gttaacgcct ggtgggcgga
    19801 aacgctgcaa aaacaacggc tgaccagcgc attagaactg gagtatgaaa ctcatttctg
    19861 ccgttttctg atgccaacca ttcgcggagc cgataccggc agtaaaaagc gctacgccgg
    19921 gctgattcag gagggcgata aacagcggat ggtgtttaaa gggctggaaa ccgtgcgcac
    19981 cgactggacg ccgctggccc agcagtttca gcaggagcta tacctgcgca tcttccgcaa
    20041 cgagccatat caggaatatg tacgcgaaac catcgacaaa ctgatggcgg gtgaactgga
    20101 tgcgcgactg gtttaccgta aacgccttcg ccgtccgctg agcgagtatc agcgtaatgt
    20161 gccgcctcat gtacgcgccg ctcgccttgc cgatgaagaa aaccaaaagc gcggtcgccc
    20221 cttgcaatat cagaatcgcg gcaccattaa gtacgtatgg accaccaacg gcccggagcc
    20281 gctggactac caacgttcac cactggatta cgaacactat ctgacccgcc agctacaacc
    20341 cgtggcggag ggaatactcc cttttattga ggataatttt gctacactta tgaccgggca
    20401 acttgggcta ttttgagcaa aaaaaagagt tcgccagata ccattttgat gcgtgacgaa
    20461 tgctttgcca tccagtacca tagcgccctt tccattcctg gacctgaata acaccactac
    20521 ctcataagca cggtagcggg tggttattgc ctgcaattaa agatatagag ccgaacacat
    20581 atgcctttta cacttggtca acgctggatc agcgatacag aaagcgaatt gggacttgga
    20641 accgttgtcg cggtggatgc gcgaactgtc actttacttt tcccatctac tggtgaaaac
    20701 cgtctgtacg cacgcagtga ttcccccgtg acccgcgtga tgttcaaccc tggtgatact
    20761 attaccagcc atgacggctg gcagatgcaa gtcgaagaag taaaagaaga aaatggcttg
    20821 ctgacctata tcggtactcg cctggatact gaagagtccg gcgtagccct gcgtgaagtt
    20881 ttccttgata gcaaactggt gttcagcaaa ccgcaggacc gtctgtttgc cgggcagatt
    20941 gaccgtatgg accgctttgc gctgcgttat cgcgcgcgta agtattccag cgaacagttc
    21001 cgtatgccgt acagcggcct gcgcggtcag cgtaccagcc tgattccgca tcagctcaac
    21061 atcgctcatg atgttggtcg ccgccacgcg ccgcgcgtcc tgctggctga cgaagtgggt
    21121 ttagggaaaa ccattgaagc cgggatgatc ctgcatcagc aactgctctc tggcgctgct
    21181 gaacgtgtgc taattatcgt cccggaaacc ttacagcatc agtggctggt agaaatgctg
    21241 cgccgtttca acctgcgctt tgcgctattt gatgatgagc gttatgccga agctcagcac
    21301 gatgcttaca acccgtttga caccgaacag ctggtgattt gctcgctgga ttttgcccgt
    21361 cgtagcaaac agcgcctgga acatctctgt gaagccgaat gggacctgct ggtggtcgat
    21421 gaagcgcatc acctggtgtg gagcgaagat gcgccaagcc gtgaatatca ggccattgaa
    21481 caactggcag agcacgtgcc gggcgttctg ctgctgaccg cgaccccgga acagctgggg
    21541 atggaaagcc acttcgcccg tctgcgtctg ctggacccga accgtttcca cgattttgcg
    21601 cagttcgttg aagagcagaa aaattatcgt ccggttgcgg acgccgttgc catgctgctg
    21661 gcaggtaaca aactgagcaa tgacgaactg aacatgctcg gcgagatgat cggcgagcag
    21721 gatatcgagc cgctgttgca ggcagcaaac agcgacagcg aagatgccca gagcgcccgt
    21781 caggagctgg tttcgatgct gatggatcgc cacggcacca gccgcgtgct gttccgtaac
    21841 acgcgtaacg gtgtgaaagg attcccgaaa cgcgagctgc acaccattaa actgccgcta
    21901 ccgacgcagt atcagacggc tattaaagtc tccggcatta tgggcgcacg taaaagtgcg
    21961 gaagatcgtg ctcgcgatat gctctacccg gagcgtattt atcaggaatt tgaaggtgat
    22021 aacgccacct ggtggaactt cgatccgcgc gttgagtggc tgatgggcta cctgaccagc
    22081 catcgctctc agaaagtgct ggtgatctgc gccaaagctg ccactgcgct gcaactggag
    22141 caggtactgc gcgaacgtga aggtattcgc gctgcggtgt tccacgaagg tatgtcgatt
    22201 atcgaacgtg accgcgctgc cgcctggttt gccgaagaag acaccggcgc acaggtactg
    22261 ctgtgctcag aaatcggttc tgaaggacgt aacttccagt tcgccagcca catggtgatg
    22321 tttgacctgc cattcaaccc ggatctactg gagcagcgta ttggtcgtct ggatcgtatc
    22381 ggccaggcgc acgatattca gatccatgtg ccttatctgg agaaaaccgc tcagtcggtg
    22441 ctggtgcgct ggtatcacga aggtctggat gcatttgagc acacctgccc gaccggacgc
    22501 actatttacg atagcgtata caacgatctg attaactatc tggcttcacc ggatcaaacc
    22561 gaaggctttg acgatctgat caaaaactgc cgcgagcaac atgaagcgct gaaagcacaa
    22621 ctggaacagg gtcgtgaccg cctgctggaa atccactcca acggtggcga aaaagcccag
    22681 gcactggcag aaagcattga agagcaggat gacgatacca acctgatcgc cttcgccatg
    22741 aacctgttcg atattatcgg tatcaatcag gacgatcgcg gcgacaacat gatcgtgctg
    22801 acgccgtccg atcatatgct ggtgccggac ttccctggcc tgtcggaaga tggcatcacc
    22861 atcacctttg atcgtgaagt ggcgctggcg cgtgaagatg ctcagtttat tacctgggaa
    22921 catccgctga tccgcaacgg tctggatctg atcctttctg gcgataccgg tagcagcacg
    22981 atttcactgt taaaaaacaa agcgttgccg gtaggtacgc tgttggtgga actgatttac
    23041 gtggtcgaag cccaggcgcc gaagcagttg cagctcaacc gcttcctgcc accgacgccg
    23101 gtacgtatgc tgctggataa aaacggcaac aacctggcgg cccaggtgga gtttgaaacc
    23161 tttaaccgcc agcttaacgc ggttaaccgt cacaccggca gcaaactggt taacgccgtg
    23221 cagcaggatg ttcacgctat ccttcagctg ggtgaagcgc agatcgagaa atctgcccgt
    23281 gcattgattg atgccgcacg taacgaagcc gacgaaaaac tgtctgccga gctgtctcgt
    23341 ctggaagcgc tgcgcgcagt gaacccgaac attcgtgacg acgaactgac cgccattgag
    23401 agcaaccgtc agcaggtaat ggaaagcctg gatcaggcag gctggcgtct ggatgccctg
    23461 cgtttgatcg ttgtaacgca tcagtaacgg agccgaaaat ggggatggaa aactacaatc
    23521 caccgcagga accctggttg gttatcctgt atcaggatga ccacattatg gtggttaaca
    23581 agccgagcgg tttgttgtct gtgccgggtc gtctggaaga gcacaaagat agcgtgatga
    23641 cgcgcattca gcgtgattat ccgcaggcag aatcggtgca tcgtctggat atggctacca
    23701 gcggcgtgat tgtagtggcg ctgaccaaag ccgcggagcg ggagttaaaa cgccagttcc
    23761 gcgagcgcga gccgaaaaag cagtatgtgg cccgcgtctg ggggcatcca tcccccgcag
    23821 aaggtctggt ggatctgccg ctgatttgcg actggccaaa ccgcccgaaa cagaaagtct
    23881 gttacgaaac gggtaaacct gcgcagacgg aatatgaagt ggtggagtat gcggcggata
    23941 acacggcaag agtggtgtta aaaccgatta ccgggcgttc gcatcaattg cgtgtgcata
    24001 tgctggcgct ggggcatccg attctcggcg atcgttttta tgcatcacca gaagcgagag
    24061 cgatggcacc gcgtttgttg ctacatgcag agatgttgac gattacccat ccggcgtatg
    24121 gcaatagtat gacgtttaaa gcgccagcgg atttttaatc gtcacgctgg cgcagttcgg
    24181 taggcggata aggcgttcac gccgcatccg gcaattgtgc gccgattgcc tgatgcgacg
    24241 cgggccgcgt cttatcaggc ctacagttta aaacttattt aaaccctttc tgctgcttta
    24301 tcagctcata tgcctgctga atttcctgcg ctttctgctt cgccatctcc atcatctcag
    24361 gcggcaaacc tttcgccacc agcttatcgg gatggtgttc actcatcagc ttacggtagg
    24421 cacgtttgat ggtggtcgca tcatccgtcg gcttcacgcc cagcacatta caggcatctt
    24481 ccagcgtcgg gccacgctgc gcttgctgcc agttaccacc gccagattgc tgctgataac
    24541 cgccgccaaa ctgtgcaccg ccctgcatca tgcgcaaaaa ctggtcaaac tgggcgcggg
    24601 agatccctaa ttcttcagca atgacataca gcaccgcccg ttcattcggg tgcagtgaac
    24661 catcagcaaa cgccgcctga atctggatct ccagaaacat acgaattaag tcaaaacgac
    24721 caaagcagac actgcgaaac tggcgcatct tttcgcgcag cgggtaattg tctgattttc
    24781 ccacccggaa cgcattttgc gccgcagtac gggaagcgcc atgaagattc attcggtcca
    24841 tcaactggct ggcgatatga atatcagcct ccgtgacgcg acctttggat ttggttaaat
    24901 gccccatcac ttcaaaagtg gtggcaaaaa acagcgcctg acgctcacgc tggttggcga
    24961 accacgccat tttacggcta cgggctttat caaacatatg gccaattaac aggcctaaca
    25021 ctacgcccca aaagccgccg cccatcagta aggccacggc cacgccaatg atttttcccc
    25081 aatactgcat atattcccca aatcgacaca cggatatcag ggctatctcc cacaatataa
    25141 aggtgctttt accgttttcc ggctgcggtc aattgtggga catcgcctat aatttgcatt
    25201 atcatacctg tcattcaatg ccgtgcctaa caccactgac gctattcgga caggattaac
    25261 actagcgtag agatgacgag tacgttagtc tctgaccgtt tgtcacgcgc aacgttaccg
    25321 atgatggaac aataaaatca acgtatgaaa aaacgtatcc ccactctcct ggccaccatg
    25381 attgccaccg ccctttatag tcaacaggga ctggcagccg acctcgcctc acagtgcatg
    25441 ttgggcgtgc caagctatga ccgtcctctg gtacagggcg ataccaatga cttacccgtg
    25501 actatcaatg ctgaccacgc gaaaggggac tacccggatg acgccgtgtt tactggcagc
    25561 gtggatatca tgcagggtaa cagccgtctg caggccgacg aagtgcagct ccatcaaaaa
    25621 gaggcaccag gacaaccgga gccggtacgt accgttgatg cgctcggtaa tgtccattac
    25681 gacgataacc aggtgatcct caaagggccg aaaggctggg cgaatctgaa caccaaagat
    25741 accaacgtct gggaaggtga ttaccagatg gtgggtcgcc agggtcgcgg taaagcggac
    25801 ctgatgaaac aacgtggcga aaaccgctat accattctgg ataacggtag ctttacctcc
    25861 tgtctgccgg gttctgacac ctggagcgtg gtaggtagcg aaattattca tgaccgcgaa
    25921 gaacaagttg cggagatctg gaacgcccgc tttaaggtgg gtccggtacc gatcttttat
    25981 agcccctatt tgcagttgcc ggtgggtgac aaacgtcgct ctggtttctt gatcccgaac
    26041 gccaagtaca ccaccaccaa ctactttgag ttctacctgc catattactg gaacatcgcg
    26101 ccaaatatgg atgccaccat cacgccgcat tatatgcatc gtcgtggcaa catcatgtgg
    26161 gagaacgaat tccgctacct ctcccaggcg ggcgctggct tgatggaact ggactatctg
    26221 ccttcagata aagtctatga agatgaacac ccgaacgatg acagttcacg tcgttggtta
    26281 ttctactgga accactccgg ggtcatggat caagtgtggc gtttcaacgt cgactacacc
    26341 aaggtcagcg atcctagcta cttcaatgat ttcgataaca agtacggttc cagtactgac
    26401 ggctacgcaa cgcaaaaatt cagcgttggc tatgcggtgc aaaacttcaa tgccaccgtt
    26461 tcaaccaagc agttccaggt tttcagcgaa cagaacacca gtagctactc ggcagagccg
    26521 cagttagacg ttaattacta ccagaatgat gttggtccgt ttgatacgcg tatttacggc
    26581 caggcagtgc actttgttaa caccagagac gacatgcctg aagcaacccg tgttcacctg
    26641 gaaccgacca tcaatttgcc gctctctaat aactggggca gcatcaatac cgaagcgaag
    26701 ttgctggcaa cccattatca gcaaaccaat cttgactggt ataactccag aaacacgacc
    26761 aagctggacg aatccgttaa ccgcgtaatg ccgcaattca aagttgacgg caaaatggtc
    26821 tttgaacgcg atatggaaat gctggctccg ggttataccc aaacgctgga accgcgcgcg
    26881 cagtatttgt acgtgccgta tcgcgatcag agcgacatct ataactacga ctcgtctctg
    26941 ctgcaatctg actactctgg cctgttccgg gaccggactt acggcggtct tgaccgtatt
    27001 gcctccgcta accaggtgac gaccggtgtc acatctcgca tatatgatga tgctgccgtt
    27061 gaacgtttta atatttccgt tggtcaaatc tactatttca cggagtctcg cactggcgat
    27121 gacaacataa catgggagaa tgacgacaaa acgggttcac tggtgtgggc aggcgatact
    27181 tactggcgta tctccgagcg ttggggattg cgtggcggga ttcagtacga tacacgtctg
    27241 gataacgtag cgaccagtaa ctccagcatt gaataccgtc gggatgaaga ccgtctggta
    27301 cagctgaatt accgttacgc cagcccggaa tatattcagg ctacgctgcc taagtactat
    27361 tccactgctg agcaatataa gaatggtatt tcgcaggtag gtgctgtcgc cagctggcca
    27421 attgccgatc gttggtccat tgttggggcc tactactacg acaccaatgc gaacaagcaa
    27481 gccgactcta tgttaggtgt gcaatacagc tcctgctgct atgcaattcg cgtcggttac
    27541 gagcggaagc tgaacggttg ggataacgat aaacaacatg cggtatatga caacgcaatc
    27601 ggctttaaca tcgaacttcg cggcctgagc tccaactacg gtctgggtac gcaagagatg
    27661 ctgcgttcga acattctgcc gtatcaaaac actttgtgat ttgttgattt accacgtaat
    27721 ccgcagtgcg gttaattgaa atggaaaaag tatgaagaac tggaaaacgc tgcttctcgg
    27781 tatcgccatg atcgcgaata ccagtttcgc tgccccccag gtagtcgata aagtcgcagc
    27841 cgtcgtcaat aacggcgtcg tgctggaaag cgacgttgat ggattaatgc agtcggtaaa
    27901 actgaacgct gctcaggcaa ggcagcaact tcctgatgac gcgacgctgc gccaccaaat
    27961 catggaacgt ttgatcatgg atcaaatcat cctgcagatg gggcagaaaa tgggagtgaa
    28021 aatctccgat gagcagctgg atcaggcgat tgctaacatc gcgaaacaga acaacatgac
    28081 gctggatcag atgcgcagcc gtctggctta cgatggtctg aactacaaca cttatcgtaa
    28141 ccagatccgc aaagagatga ttatctctga agtgcgtaac aacgaggtgc gtcgtcgcat
    28201 caccatcctg ccgcaggaag tcgaatccct ggcgcagcag gtgggtaacc aaaacgacgc
    28261 cagcactgag ctgaacctga gccacatcct gatcccgctg ccggaaaacc cgacctctga
    28321 tcaggtgaac gaagcggaaa gccaggcgcg tgccattgtc gatcaggcgc gtaacggcgc
    28381 tgatttcggt aagttggcga ttgctcattc tgccgaccag caggcgctga acggcggcca
    28441 gatgggctgg ggccgtattc aggagttgcc ggggatcttc gcccaggcat taagcaccgc
    28501 gaagaaaggc gacattgttg gcccgattcg ttccggcgtt ggcttccata ttctgaaagt
    28561 taacgacctg cgcggcgaaa gcaaaaatat ctcggtgacc gaagttcatg ctcgccatat
    28621 tctgctgaaa ccgtcgccga tcatgactga cgaacaggca cgcgtgaaac tggaacagat
    28681 tgctgctgat atcaagagtg gtaaaacgac ttttgctgcc gcagcgaaag agttctctca
    28741 ggatccaggc tccgctaacc agggcggtga tctcggctgg gctacaccag atattttcga
    28801 tccggccttc cgtgacgccc tgactcgcct gaacaaaggt caaatgagtg caccggttca
    28861 ctcttcattc ggctggcatt taatcgaact gctggatacc cgtaatgtcg ataaaaccga
    28921 cgctgcgcag aaagatcgtg cataccgcat gctgatgaac cgtaagttct cggaagaagc
    28981 agcaagctgg atgcaggaac aacgtgccag cgcctacgtt aaaatcctga gcaactaatg
    29041 gttaaaaccc aacgtgttgt gatcactccc ggcgagcccg ccgggattgg cccggactta
    29101 gttgtccagc ttgcacagcg tgagtggccg gtcgaactgg ttgtttgtgc cgataccact
    29161 ctccttaccg accgggcagc gatgctcggt ttgccgctca ccctccgccc ttattccccc
    29221 aactcccctg cacaaccgca aactacgggc acattaacgc tacttcctgt cgcgctacgt
    29281 gaatctgtca ctgcggggca gttaggggtt gaaaatgggc attacgtggt ggagacgctg
    29341 gcgcgagcgt gtgatggctg tctgaacggt gaatttgctg cgctgatcac aggccccgtg
    29401 cataaaggcg tcattaacga cgcaggtatt ccgtttactg gtcataccga gtttttcgaa
    29461 gagcgttcgc aggcgaaaaa agtggtgatg atgctggcga ccgaagaact tcgcgtggcg
    29521 ctggcaacga cgcatttacc gctgcgcgat atcgcagatg ctatcacccc tgcacttttg
    29581 cacgaagtga ttgctatttt gcatcacgat ttgcggacaa aatttggtat tgccgaaccg
    29641 cgcattctgg tctgcggact gaatccgcac gcgggcgaag gcggtcatat gggtacggaa
    29701 gagatagaca ccattattcc ggtgctcgac gagctgcggg cgcaggggat gaaactcaac
    29761 gggccgctgc ctgccgatac cctgtttcag ccgaaatatc tcgataacgc cgacgccgtg
    29821 ctggcgatgt accacgatca gggtcttccc gtgctaaaat accagggctt cgggcgcggt
    29881 gtgaacatta cgctgggcct gccctttatt cgcacatcag tggaccacgg caccgcgctt
    29941 gaactggcgg gacgtggtaa agccgatgtc ggcagtttta ttacggcgct taatctcgcc
    30001 atcaaaatga ttgttaacac ccaatgaata atcgagtcca ccagggccac ttagcccgta
    30061 aacgcttcgg gcaaaacttt ctcaacgatc agttcgtgat cgacagtatt gtttctgcca
    30121 ttaacccgca aaagggccag gcgatggtcg aaatcggccc cggtctggcg gcattgaccg
    30181 aaccggtcgg cgaacgtctg gaccagctga cggtcatcga acttgaccgc gatctggcgg
    30241 cacgtctgca aacgcatcca ttcttaggcc cgaaactgac gatttatcag caggatgcga
    30301 tgacctttaa ctttggcgaa ctggccgaga aaatgggtca gccgctgcgt gttttcggca
    30361 acctgcctta taacatctcc acgccgttga tgttccatct gtttagctat actgatgcca
    30421 ttgccgacat gcactttatg ttgcaaaaag aggtggtgaa tcgtctggtt gcaggaccga
    30481 acagcaaagc gtatggtcga ttaagcgtca tggcgcaata ctattgcaac gtgatcccgg
    30541 tactggaagt tccgccgtca gcctttacac caccacccaa agtggattcc gccgtcgtgc
    30601 gcctggttcc tcatgcaacg atgcctcacc cggttaaaga tgttcgtgtg ttgagccgca
    30661 tcaccaccga agcctttaac cagcgtcgta aaaccattcg taacagcctc ggcaacctgt
    30721 ttagcgtcga ggtgttaacg ggaatgggga tcgacccggc gatgcgagcg gaaaatatct
    30781 ctgtcgcgca atactgccag atggcgaact atctggcgga gaacgcgcct ttgcaggaga
    30841 gttaacgatg atcaattcgc cccgagtgtg tattcaggtt caaagcgtct acattgaggc
    30901 acaatcttca cctgataatg aacgttacgt ttttgcttat accgtaacca tacgcaatct
    30961 ggggcgagcg ccagtgcagt tgttggggcg ttactggctg atcaccaatg gcaatggccg
    31021 tgaaaccgaa gtccagggcg aaggagtggt tggcgtccag ccacttatcg cgcctggcga
    31081 agagtaccag tacaccagcg gtgcaatcat tgaaaccccg ctgggcacca tgcagggtca
    31141 ctacgaaatg atcgatgaaa atggcgtccc tttcagcatc gacattcccg tattccgact
    31201 cgccgttccc acactcattc attaaaagaa tatggcgaca taccttattg gcgacgttca
    31261 tggttgttac gatgaactga tcgcattgct gcataaagta gaatttaccc ctgggaaaga
    31321 taccctctgg ctgacgggcg atctggtcgc gcgcgggccg ggttcgctgg atgttctgcg
    31381 ctatgtgaaa tccttaggcg acagcgtacg tctggtgctg ggcaatcacg atctgcatct
    31441 gctggcggta tttgccggga tcagccgcaa taaaccgaaa gatcgcctga caccgctgct
    31501 ggaagcgccg gatgccgacg agctgcttaa ctggctgcgt cgccagcctt tgctgcaaat
    31561 cgacgaagag aaaaagttgg tgatggccca cgcagggatc acgccgcagt gggatctgca
    31621 gaccgccaaa gagtgcgcgc gcgatgtaga agcggtgctg tcgagcgact cctatccctt
    31681 cttccttgat gccatgtacg gcgatatgcc aaataactgg tcaccggaat tgcgggggct
    31741 gggaagactg cgttttatca ccaacgcctt tacccgtatg cgtttttgct ttccaaacgg
    31801 tcaactggat atgtacagca aagaatcgcc ggaagaagcc cctgccccac tgaaaccgtg
    31861 gtttgcgatt cctggccctg tcgctgaaga atacagcatc gcctttggtc actgggcatc
    31921 gctggagggc aaaggtacgc cggaaggtat atacgcgctg gataccggct gctgctgggg
    31981 tggtacatta acctgcctgc gctgggaaga taaacagtat tttgtccagc cgtcgaaccg
    32041 gcataaggat ttgggcgagg gagaggcggt cgcgtcttaa cgaattacca ttcagggagg
    32101 aataatggct gactattttg aaatacgtgc tgattacaat cagcacacca tcacggttta
    32161 tcaggcttac aatgatgcaa tcgctgacgt cgccgtacgt gacgggcgat ttggcgcatc
    32221 tttttcattc aaccgcatga cctggattaa accctctttt atgtggatga tggagcgtag
    32281 caactgggga ctgaaaaaag atcaacaaca cattctcgca atccgaatca aacgcacctt
    32341 ctttgataca ttgctggaac aagcggtgct aaccacgccg gaagcccatg tgtatccaca
    32401 tgcaggcatt tgggaaacgt tatttgcaca agcaaatgtg tatgttcagt gggatcccga
    32461 acgcagtata aatggtaaaa aactggagca ccgatcatta cagcttggta tttcccgtaa
    32521 cttgatcagt caatttaacg aagacgccat tgttgccatt gatgatctga ctccgctggt
    32581 gcgaaagtgt cataaccttt tgataaacgg taaaacaaca caggcgaaat ccttcctgcc
    32641 gccagaaaaa atttaccctg ttagtgcagc tgcgagaaag gctttgggaa tgaaaaattc
    32701 ctgagacaaa tacgcgccta ttaagaggaa aatatctaag tgcgacaaat caacagcggg
    32761 cttaatacca gagaaattat tctggcaatg attgacgaag cagatgaacc cccatttgct
    32821 gagcagcgga cctggcatcg caggcgaatc gcttttctat gtgattgcat ccgcgttgct
    32881 atcgactacg gcttcttctg ttctgtctct cagcagtcaa taatgaacat aaccaggatg
    32941 gctgaagatg tatggataac agaaagtaaa acgacccaac tcattgatat ttggcaacaa
    33001 cttaagcagc tttatccgcc agaaactatt ccctacgtcc ataagttacc cactaaggaa
    33061 ttacttgaac tgctactttc tagtcctgat gatcttcgaa taattgaaat tcctgacatc
    33121 atcggtgatg tttttgatga ccaaacctgg attcggttat tcaaaaaaca ctttgctgat
    33181 attatccaac tacgatgtga tgtgtaacaa tccaaaatat atcgccagac gatagaccaa
    33241 aaagaaagta tttagcagat actcaacatt ctgacttctg tacagaaata gcagagttta
    33301 aaaacttaaa acttccggta ctcatcagag aaacatcctg tttcttctgc aaattcattt
    33361 aatgccgaga tggcatcctc gtttacttca ttccactgta gagtggcttg aaggtttaga
    33421 cctacgcaat gagcgcgact cacaaaatcg cgctcactgg taaaatcaaa cgtcttatcg
    33481 ttcaatttgt agtcctggca aaaaattacc gccgctccag aatctcaaag caatagctat
    33541 gagagttctg cgcatcggcg tcgtggaatt cgctgaatac cgattcccag tcatccggct
    33601 cgtaatccgg gaaatgggtg tcgccttcca cttctgcgtc gatatgcgtc agatacagtt
    33661 tttgcgcttt tggcaggaac tgctcataaa cgcgaccacc gccaatcacc atgatttctg
    33721 gtacgtcacc acacgccgcg atggcttcat ccaccgactt cacccacgtt acgcgatcgt
    33781 ccgtacccgg ttgactgctg aggataatat ttttgcgtcc tggcagcgga cgaccgatgg
    33841 attcccaggt atggcggccc ataattacgg gtttatttaa ggtgttgcgt ttaaaccagg
    33901 cgagatcggc aggcaggttc cacggcatgg cgttttccat gccgataaca cgatctaccg
    33961 ctaacgccgc aatcagacta atcattgatt tatcctggat ataaaaaatt gtcgccacta
    34021 tacgtaaagc ataaaccgtc gtcgactggc gcaaggatga tgttgagtaa aattttacgt
    34081 tctgctggcg agtccacgct ctcccactgg actcgccaca ttacaatgaa acaaaaacaa
    34141 acagttagct gtaaagtgtg atttacgtca ctctttatta ggatgagggt ttcgtttccg
    34201 gttcatccgc catgttgccg gtatgtttcc cttcttcggt tccctgccag ccatgtcgtt
    34261 gaattaatga cagatgttcg cggtcttcgg taatgatctc acttaacatc gcgctggtgc
    34321 gtttatagac cgctgcgcgg gctttggtgt cgttctcaac cattgccatc tcttccacca
    34381 tctgaatatt aaagcggcgg aacacatcgg cacgttctcg cgcttcatac ggccccagac
    34441 ctaaactttc cagtgccaga cgcccggttt tcagcgcacc ttcgaaggtt tcacgctccg
    34501 gcttttcaac gcctgcctga cgcaaacgaa tgtagtgatc gacatcgcgg gcgcgggcaa
    34561 taatctgcaa atgcgggaaa tgttctttca ccatctccgt cagttgcagg ttggtttgcg
    34621 gatcgtcaat ggcgttaatc agcacttccg ctttcgccgc tccggcagac tccagtaaat
    34681 ccatccgcgt ggcatcgcca taaaacactt tcataccaaa tttacgcaag gtttcgatat
    34741 ggtccgggtc gtgatcgagc accaccattt tcaccccgct ggagagcagt aaacgtccgg
    34801 tgatctgccc gaaacgaccg aatccggcga taatcacgcg tggctgttct tcgtcgatct
    34861 catcggcttc acgcgcttcc tcagtagaag attgctcaag gcgattgagg atcaccagca
    34921 gaatcggcgt tactgccatc gacagcgcca ccgccagggt cagcgatttc gcccactccg
    34981 gctccagcac attcgccatc tgcgccgcac caaataccac aaaggcaaac tcactaccct
    35041 gccctaacaa caccgcaaac caacgacgct gtttatttgg cacttgcaac ggtcgggcaa
    35101 tcagccacag catggcgatt ttgatgatga ggaaaccgag cagcaaaatg acaatgcgca
    35161 atgggttttc aagcagcgtg ccaaagtcta tcgacatgcc aacaccgatg aaaaacagcc
    35221 ccaacagcaa acctttaaat ggttcgatat cgctctccag cgcatgacgg tattccgagc
    35281 ttgccagcag tacgcccgcc agaaacgcgc ccatcgccat cgacaagccg acctcttcca
    35341 gcagcaaacc aaagccaaac acgaggaata acgccacggc actaaacact tcccgcaagc
    35401 cagagcgggc tacaaaacgc agcgccggac gcgtgacata gcgccccagc aataccacca
    35461 gcaccagcgc acccgccact tttaacgccg agagagcaaa tgcgcccatc gtcgtcgagg
    35521 cactgctcgt tgccagtagc ggaatcatcg ccaccagcgg gatcgccgcg atatcctgga
    35581 acagcagcac cgcaaaggca ctgcgaccca tttgcgtcac catcagattg cgttcattca
    35641 tcgcctgcat ggcaatcgcc gtagaggaga gcgccagcgt catgccgatc aattccgcga
    35701 cctgccagcg cagcccaagt aacatgcaga acagccccag caggccaccg caaatcacca
    35761 tctgcaatgc gccgccgccg aacactgccg cacgcagctt ccatagcctt tgtggatcga
    35821 gttcgaggcc gataataaac agcatcagca ccacgccaat ctcggcaaag tgcaggatag
    35881 attcggcatc ggtcaccagt cgcagccccc acgggccaat aatgcagccg gcaatcaggt
    35941 agccaagtac cgatcccaga ccaagacgta ccgcaatggg tacaatcagc gccgccgatc
    36001 cgaggtagat cagcgcctga accagcgtat ggctatccat gatgggcctc ctgccattcc
    36061 agcagacgtt gcttatagtg acgcgcctgc ccttcgaggg tttcgtcgtc acaaataaag
    36121 gtgcagtgca tggcaaacgg tggcagccag ttcagcccgc agtagattgc cgtcgcctgt
    36181 agcggctgcg acagcacatc aaagcccgga tgcgcaccaa tttcaaaatg gctttccccg
    36241 ccgccggtcg tcaccgccca cagcaaatgt ttgccatgca gcgccgtgcc gccgtgaccg
    36301 taagcccagc cgtgcgagaa aactttatcg atccaaagtt tgaggagcgg tggaatgctg
    36361 taccactgca tcgggtgctg ccagacgatc agatcggcgc gagacagcgc ctcctgctcg
    36421 gcggcaatat cgatattgaa gtcaggatag agttgataaa gagagcgaat ttcgacgcct
    36481 tccagcgtcc ttgcctgttc aagcatccgt ttattcgcat gggaatgatg cggatacgga
    36541 tgcgcataaa ttataagaat cataggtagc ccgcgtgatt acgcctttgt tttcccacaa
    36601 agtgtagtca tatattcacg cggcttatag tcaataatat tgattcagaa aagagagaaa
    36661 tctgaacaat ttaccccatt ctctgcccgc ggatcgtcat attgctggtt tgcgccagtg
    36721 acatccctcg agtctccggg gcaaacgcta cggaaatcaa caagccaaac agcgagatac
    36781 ccgcccccat tagcatcgtg ttactgatac cgtaattatt gataaagatc ggcagtgccc
    36841 aggtcgaaac aatggtgcca atacggctta aggacataat cacgcccacg gcagaggcgc
    36901 ggatatctgt cgggaagagt tcattaggat agagccactg caaattaccc ggcccgccag
    36961 agaaaaaggc atacaccgca aaggccatca ctaccagcca gatccccata tccgggatca
    37021 gccccaaaac cgccagcgcc agcgtcatca tggcaaagct gccaatcaac aatggacgcc
    37081 gtccggcagt gtttagccac agcatcggcg gaatacagcc gagcataaag aacaggctaa
    37141 tcaccacgtt ccccagtgcc gcgtttttgc caacccccaa tcccaacaaa ccaacgattt
    37201 gcgggccaaa ggtgtaaatg gcgaacattg ggatcacctg gcaggtccag atggcggcaa
    37261 caaacagaac aaaaggaaaa tggcggcgat taaacagatc gcgaaaacgg gtttgctgcg
    37321 gctgctcttc atcgaaagcc accggttcgc caaacagttt tatcatcatc tcttcgcact
    37381 cttttactcg ccctttgcgt aataaccagc ggggagattc aggcagttcg aatcgaccaa
    37441 tcaaaatcaa caaacagggg atcgccgcgc tacccagcat ccagcgccag ccgccttcca
    37501 catcataaag ccagtagccg accagatcgg cgcaggtcgc gccgacatac cacatggcgg
    37561 cgataaagct gatggaaaac gcccgctgac gggtactgga gaactcggtg atcattgagg
    37621 tggcgatggg ataatctgca ccgatgacaa tgccgataag tacccgcatc accaacagtt
    37681 cgacagggga tgagacaaac atcgtcgcca ccgatatcac gccgatggcg atgatatcaa
    37741 tgaggaacat tttgcgccgt ccgactttat cggagatata gccaaacagc gatgtgccaa
    37801 cgaacagccc ggcgagcgtt cccgcaccca gcaagccaat ccagtcagcg tccagtttca
    37861 gcgccggagt cagttgctcc agcgccacgc caatcattac cagtacataa ccatccagaa
    37921 acggaccacc acttccccac agcaaaatgc ggcggtgaat agaggagaat ttgagatcgt
    37981 caaagtttct ggacggttgc atgggttatt cctgtttttt gcaatgagag tcagtgcggc
    38041 gttacgtatc aaaccaacat cagccgtaac ggaactccac accaaaggtg ccgcgcgggt
    38101 attcccactg ttccagcgcc gaccccagcc ccagaatgcg acaggtgccg cactccagac
    38161 atccggcgta atcgaagcgc acactgccgt catcctgctt cttgtacaga cctgcggggc
    38221 acgctttcac cagcagctcc agcacctgtt tatcagcatc ggccttcaca acaatgtgcg
    38281 gatgctcttc atcgacattg aatttattga cgcccagttt gacgtccaca ttgacgggag
    38341 aagtcataaa acggtcactc ctttcatgcc atccttgatt agattgatga agcccacttt
    38401 cttaccgtgg cggaggattt tcttgcgcat cagttctggc gcactgccgt caatggtgaa
    38461 caggtcacgc gccacgccca ccgccagctc cgggtagccg ctgaacatgc gtgggttatc
    38521 aaggaacgcc ggtagtttct ggtacatgcg catatcgcgc agcgggccac tctcaagatg
    38581 ctgacgatat tccgccagtt tttgcttact gaaatcgtcg cttttcatcg ctgaaagcac
    38641 ggtttttgct gcagcttccc cggcggcaat cgccagatcc ataccgcgaa tggtaaaacc
    38701 gaggttcata cacattccgg cggcatcgcc ggcaatcaat acgccgtcac cgaccagctc
    38761 cggcagcatg ttgatgcctg cttccggcac tacgtgagcg gaatattcca ccagcttgcc
    38821 acccgcgatc agcggtgcaa cggccggatg ttgtttgaaa tcttccagca tttgcggcac
    38881 cgattttttt gcgtcatgca gatgatgcag accacaaacc agccccagcg acagggtatt
    38941 ttcattggta taaaggaagc cgccgcccat caggccatcg gtgggtgatc ccgcaaacag
    39001 acaagccgcg ccctgattac cctgcaactg aaaacggtct tcgattaccg acttcggtaa
    39061 ctcgatcagt tccttcacgc caaccgccac atccgtcggt ttaacgcgtt ttgccatccc
    39121 caacttttcg gcaaggatgg aattcacccc gtcagcaagg atcaccgttt tcgcttcaat
    39181 cacatcgcca tcggcttcta caccgacgac tttgccatcg cgctgtacga ggttatcgac
    39241 acgaatcccg gtaattaact gcgcgcccgc ttcttcggcc tgctccatca accaggcatc
    39301 aaatttactg cgcaaaacgg agtaagaacg ctgggatggc gaggtttcgt caccgttgca
    39361 gtagtccata gtcatcgctg acttttccgt cataaacgcg agtttttcat gggtgatcag
    39421 gcgttctacg ggggcggagt cggcgaaacc aggaataatg tgttccagac tgtgggcata
    39481 gagacgcccg ccggtgacgt tcttggcacc tgcggaattg ccacgctcga taactaacac
    39541 ttgcgcacct tcgcgggcga gcaccagtgc ggcaaccgaa ccggcaagcc ctgcaccgac
    39601 gatgatggcg tcaaagatat cttcggacat aagagctccc tgtcagcggc agggccaaaa
    39661 tgcagccctg ccagagtgga tcaacgcgct aaagctgcgg tcagcgccgg aaggatcttc
    39721 acggcgtcgc caacaatgcc gtaatccgcg tactggaaga tcggcgcatt tttatctttg
    39781 ttgatggcga aaatggtttg cgatgcatta gcaccaacca tgtgctggat ctgcccggag
    39841 atccccaccg ccaggtacag ttcaggtttc agcatcaggt tggagatacc gacatagcgt
    39901 tcgtgctcca tccatttttc gttttccgcc accggacgag aacaggccaa ctccgcacct
    39961 atcgccttgc aaagctgttc tgccagcgca atgttctctt tgctgccaat accgcgaccg
    40021 acgctgacca ccagacgggc tttgtcgaga tcgacgctgt tgctctggcg cgcctgggtt
    40081 gccgtgcggg taatcgccac agccggagcc tgccactcca cggtgtgcgt ttcgccagtg
    40141 cgtgacgcgt ctggctgagc cgcatcgaac gtgccgctgc tgatggtcag taccgcatac
    40201 ggcgtggcaa tgcgttcttc gccaatcgcc agaccaccgt aaaccatgtg tttcactgtc
    40261 gctttaccgt cctgtacgct gacggtgctg gcatcgttag acaccgccgc tttaaggcga
    40321 taacccagtt ttgccgccag taatttgccg cgacgggtgt ttggcagcag caccaggccg
    40381 tctgcgccgt gctggcgaat agtgtcagcc atgacaccgg cgtaatcttc gatcatccga
    40441 tcgtccggtt tgccgtttaa tttccagaca tgattagcgc cgagctggat tgcctgtacg
    40501 ccgtcggcat cattgaggac aaaggtgttg atttgattag ctaaagcctg cgcaccgttc
    40561 atcagttccg gcagacgaga aggggtatcg ctgaataccc agacttgaga aaacgtgttc
    40621 atagcatccc ctgtaattaa atgactttgc gaagattttc agcaaatgcg gcgatctgtt
    40681 cttcgccgtc gccttcaatc acgatgcgct gacgttcgcg ctgtttcggc gcagcaacct
    40741 gttgttcaga ccaggcgtcc acggcgttaa aaccgatatc cgccgccgac catacctgga
    40801 ctggcttttt cgccgcgccg agaatggctt tcatcgaagg aatttgtggg gagttgatat
    40861 cagtggaaac agcaacaacc gcaggcagcg gaatgcttaa ggtttcggtt tcatcttcca
    40921 gttcgcgctc aacggtgagg gtatctgccg tcagggagat aattttgctg acgccgttaa
    40981 ctgccggaat attgaggatt tcgcccacca gcagaccaac ctgctgggca taaaggtcgg
    41041 aagaaccatc gccacagagg atcaaatcaa agcctgcttt ctgggcggct gcagccagtg
    41101 cgctcgccgt ttgttgcggc agtgcctgct cgaactggtc atcaatcacc acaatcagtt
    41161 catccgggcc gcgcgatagc acatctttac gccctttggc gttggtcagg gctttaccgc
    41221 ccacacttaa ggccgtcacc tgcgcctctg ctgcctgttg ctttagctgg caagccgctt
    41281 caatagcgtt cagatcgtat tggcttattt tggcatcggc tttgctgaag tctaatgaac
    41341 catcagcatt attgaccgca atatcctgtt catcaggcac gcacttatag caagtaataa
    41401 tcttcattgc atctccagaa atcatgaagg aaacgtcgtt c
//
LOCUS       DAZGVQ010000024        41356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000024 DAZGVQ010000000
VERSION     DAZGVQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.024"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            72..806
                     /locus_tag="ACHLAG_001250"
     CDS             72..806
                     /locus_tag="ACHLAG_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP0654517.1"
                     /translation="MKSIATLVVCAISGIACVNLSAHAAEGNHTISLGYAHFQFPGLK
                     DFVKDATAHNRETFSHFVNRNYFSSLGEYTDGRVSGYEGKDKNPQGINIRYRYEITDD
                     FGVITSFTWTRSLTNSQTFIDVQSADHTRKIKNPAASARTDIRANYWSLLAGPSWRVN
                     QYMSLYAMAGMGVAKVSADLKIKDNINSSGGFSESNSTKKNSLAWAAGAQFNLNESVT
                     LDVAYEGSGSGDWRTSGITAGIGLKF"
     gene            1037..1177
                     /locus_tag="ACHLAG_001251"
     CDS             1037..1177
                     /locus_tag="ACHLAG_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0654518.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            1234..1635
                     /locus_tag="ACHLAG_001252"
     CDS             1234..1635
                     /locus_tag="ACHLAG_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654519.1"
                     /translation="MAPVSGERMNDKILRYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSADGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            1730..2386
                     /locus_tag="ACHLAG_001253"
     CDS             1730..2386
                     /locus_tag="ACHLAG_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654520.1"
                     /translation="MGGSKGGGDTKVKPTAAQIAQEEVAWKGWQDYKNILRPAEDNFM
                     EKVDDLNSEQQYDNIAGTTNLGYQKQFGEARKELAGNLAQSGVDPSSGRFNAVMNANQ
                     SDQVTGQIDTTTRGQVSQADKYVAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAVGAYAMHKAGGSGGSGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            2389..2835
                     /locus_tag="ACHLAG_001254"
     CDS             2389..2835
                     /locus_tag="ACHLAG_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654521.1"
                     /translation="MEYGKYETLARYGYTGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVSRPDNPDSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQKLSVGGQLVAA"
     gene            2845..3096
                     /locus_tag="ACHLAG_001255"
     CDS             2845..3096
                     /locus_tag="ACHLAG_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFP0654522.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRREEIEAANKQMAAQQKAQ
                     NKQNIGTGIGTGAAIGASVGGPVGAVAGAVIGGIAGSLF"
     gene            3107..4372
                     /locus_tag="ACHLAG_001256"
     CDS             3107..4372
                     /locus_tag="ACHLAG_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654523.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLARQQKNNERDFE
                     FAQSQFEHNKNVDQRNFDYRAKVDDRNYALKEREFNANQNYRNASLGMEQQRLRMQKY
                     NQRRLEYNDMLARDQPVMAALGKAVDAGDRDAAMRLYGQLSEGNPLRLMANDGYVAKA
                     GQAVNNLQKIFDDKPDRAIASLNTPENLDVLSGVFGPELQQRIGMPDSTGKKTIKEAR
                     IGSIVPAQQEGYILIGLDLTYSDGSTAHKPVTEYGSAHPDDQTVLAIPVDKAIAQVRD
                     RSKFAEISKNYGYFMPKQQGLSLKELQKGASNVAADAIKNGGNAQAAVDEYYAATGSQ
                     PHQQKIQQQKLQQQVINWAGDDPDKLSFARNVAARQPEMLEPQNQKLLENGYANFLRI
                     QKARGEQARDESASSASQFIRGLKQNYAQ"
     gene            4442..12823
                     /locus_tag="ACHLAG_001257"
     CDS             4442..12823
                     /locus_tag="ACHLAG_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFP0654524.1"
                     /translation="MAYSEEQRPEAQLGNQNRNSLNIQQPGETDSYEAFFSDPNRWKD
                     NSTSFSLGDVLPTMGKGFAQSVRGTGEMARGLGDAMIQSPVKTGARILNEFSRMGLPG
                     VATVQDIFAGGSRGADEVIDTLPDGKNAVTDTVGKGLKATGKAVSDGAEATDEWLTGK
                     MSPGAVRALNTPMTEGYDDSAVWVAKGVNLIGALVPDMVAGGVAKKVGDVTLRKMLTA
                     GLEKKYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSTASAQGQSAMAAADAVLN
                     ADYSELAQSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAV
                     NAMAAKLGDAQLFNLVTRGTAKTVKSGIVRNATEQGAINAAQGGYSRYQENTALRETA
                     GMDVSPWEGVADATIEGAAFGAAMGAPSGAVAGYRGRRQAAEEAAMRDAETVQQDDAA
                     PQPESVDPVAQHRESMQGMNREQLLEQYADADMATEGDASAAHRREAASQLLNELDEQ
                     AKRQAVMNELKAKPRSELLEEYRRLSQKEERTETEEQQFQAIREVIRPQQEVTPEAQS
                     QPENAEDGNGSIYPTVRFRDPNEVRIEINGNGASRPAERIEKVRPDNRYFTDEKSAMG
                     SDVFRNAAATGLKPSVVKKGETQYAVEMDNPAFSEDVATETINTLADGERIADADPME
                     QPAFMRDPRFRGFTGDDTEVQARLARGNVPTAEELVRSQMAEGDAGPTAQELTERPRL
                     PAPGDIHPGQGYPLPGEVARTPDENQAGRGGRFTTTGEVKGQSFQKGQALAPENAAGR
                     QGETLEGEMVRRGLPSPDAQNATAPVREGLPAPDITHNVRMPQPDQLPRTVRNSLPEL
                     AQQAEVRRQAGGNRDIPQPETIAPESETTVATDREATVRGGEVRGKKIEDFGEEIKGA
                     AKHRYAQLAETLGKTLEDRDYVTQPLSKLFPKPDYAKLASEGADADTLAMIALYRSDI
                     PVKTKHNTAGWGESVKKVRHSVSEMLNGTVSAKRLAEWMEGRMPSRYADTWQLLRTLP
                     PSQMDKASAYRVVSGVYQAAGGKRYDPPQKLYSLRNKDNKGSNLFFSESRDELLAKAK
                     VWFAEQEEKSQAKGDEKTAPSPDDKIRFDVYRNTRSGDIFIAYGKNKMRVRGGFKSAS
                     DARKYIDLHRDELVRHVKEMREISREEQRNATNRDRTGPERRKGDVSPEQFSDAFGFR
                     GVQFGNYVESPRRQADLNRAYDSLHDLADVLNVPTKALSLNGRLGLAFGARGKGKAAA
                     HYESGEVAINLTKGNGPGALAHEWFHSLDNYFGRYDVSSDGKITSGGDFMTEAQRVRR
                     IFKDGRYVDAEYPVRQEVYDAFKGVIQAIKNSDMPRRSALLDEVRSKPYWSTDVEMAA
                     RAFERYVQDKARMAGVENDYLVNIRKAPEHNTDNTYAYPTNAELDGGIREAFDHLFRT
                     LKTRETDKGVAFYSRKGMTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDS
                     DLTVRVVKSQKEAEALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGQTVREKLRHE
                     IIHHAMEHVVTPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLA
                     HMAEKQPNKFVAAWERVVALVKGVLRRTGLLKPTELNDIRLVRETIRTLGQRVREGYT
                     PREDGVGASSQYSRSGKPDPFKVPEGEGERYRDDLARMMKSLRTTDLTVNIGRTPPVL
                     RHLGAPDLPLVISRDTVRKATNGVKHVVPMDVIERLPELMHDPDAIYRSATERNAVVM
                     LLDAVDKNGDPVVSAVHMKATQKLLEVNRIASVYGTENGKKLKSMETAGLTLYRREKL
                     NPDGSLYRGLQLPKDEHSRQGSADKILYPEDIRKGPYYSRTSSLTPEETIASRFVRQM
                     QDKFQVLKAVQENIRKTGGKIDDSNNAYMAEELFHGKAENDLNVMKERYVQPLAKLLA
                     DYKIAQADLDEYLYARHAPERNAHIAKINPKMPDGGSGMTNAEAAEIMQRVRNSGKQA
                     QYDRLAGIVDDMLARRRELIREAGLEESGVVDAWQKAYRYYVPLKGQDVDGVVSLPRT
                     GKGFTIGGRESKQAMGRASRAQSPSTQAIQDLSESLIRNRKNEVGNAFLKLVQDNPDK
                     DYWQVFTDDRPDTMRTIAERKDKETGETIREVVERPVPMAMMADRYFTTKKNGKTYYI
                     KLHDPRLMRAMKNMGPETSNAVIRTLGKVNRFLATVNTSYNPEFLVSNFIRDVQTAVM
                     NLKAEQGRSDGKLKGLDNLSALAVVKDSRSAMSAVYASLRGKTLTGNGAQWQKVWKEF
                     VEDGGKTGWFNMGDLEGQQKEMDRLVSLAKGGWKGQSIGAWNSFLNLVEDANGAVENA
                     LRLSAYKHARDAGLSRQQAASLAKNMTVNFNRRGEQGALMNSLYMFANASIQGTANLV
                     RTLGHLNGEGPLLERLRWKNLNVPQKIALAAVGAGYLLGSLNRSVAGEDDDGVNWYDK
                     VPSHVKERNLVIMKSVFGGKAGEYWSIPLPYGYNVFFLLGHTAEGVAAGDLTASRAAG
                     NVVGGVLGAFSPVGSETSETLSGALLKNAAPTILRPFANLAMNENFMGAQIYQENMPF
                     GTPKPDSQLGRRSTPEAYKAFASWLNAFSGGSQYRPGAVDITPESLKFWIDYISGGTG
                     RFISKTTDAAVKSLNGIDIPEQQVPFLGKISGEVMPYADQQKMYDRMTEIAQYHAELK
                     SLTGAERTAFIDENNGKLSMNGLMQDTRKRLKDLRKQRDAIYADSSLSLAQQAAIVKS
                     VERDMKVAVDRFNREYNKKVGVD"
     gene            12947..13138
                     /locus_tag="ACHLAG_001258"
     CDS             12947..13138
                     /locus_tag="ACHLAG_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654525.1"
                     /translation="MFNEEKVAQMAAYLLKKHGGSMRFIKLMKLMYLSDRKAMESLTG
                     KGRGLNAPPQFPGIKSPCR"
     gene            complement(13225..13440)
                     /locus_tag="ACHLAG_001259"
     CDS             complement(13225..13440)
                     /locus_tag="ACHLAG_001259"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP0654526.1"
                     /translation="MPEMGERQYYVLAADFKNNYPPSIPNTQVGVYGDWFNDGNPTWD
                     DGDGEDLHLKEVTHWMPLPEPPKEVSQ"
     gene            complement(13528..14151)
                     /locus_tag="ACHLAG_001260"
                     /pseudo
     CDS             complement(13528..14151)
                     /locus_tag="ACHLAG_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208055.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(14485..14663)
                     /locus_tag="ACHLAG_001261"
                     /pseudo
     CDS             complement(14485..14663)
                     /locus_tag="ACHLAG_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14665..15615)
                     /locus_tag="ACHLAG_001262"
     CDS             complement(14665..15615)
                     /locus_tag="ACHLAG_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP0654527.1"
                     /translation="MSEINYQVLREKAEKATRGEWSLEYGENRFDGDDALIHREAAGY
                     IPICRIEGAHPESGFDEDFQMEQQANAEFIAAANPATVLALLDERERNQQYIKRRDQE
                     NEDIALTVGKLRVELETAKSKLNEQREYYEGVIADGSKRIAELEKQCAEWERKALSNF
                     EECAAMAERIEEMQTKSAPDSFGIIGENIRTQDNRITSDPMFCVYQKREIVVDADYDH
                     DRIVWVDEDGNEANKRHSRRLELLHENFREPPEKWRRVAVKDIDEFVTCCFTEQGCKD
                     YLAVNGHNLRLPFIYVKSGFRNAEYIGIRNWLAGIRIKGE"
     gene            complement(15602..15886)
                     /locus_tag="ACHLAG_001263"
     CDS             complement(15602..15886)
                     /locus_tag="ACHLAG_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFP0654528.1"
                     /translation="MSIDTTITIDTALNTGLALLGYFYIMFCAGRWLSLLLLKKWNKC
                     RKQDQRQKAMNAFSEAFGIDSMEPGDPARVISRGDVVILVYRSEEKNERD"
     gene            complement(15883..16104)
                     /locus_tag="ACHLAG_001264"
     CDS             complement(15883..16104)
                     /locus_tag="ACHLAG_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP0654529.1"
                     /translation="MADIIDNAAEIEELQRNLSLQKYKSESNAPSATHCCECGDPIDE
                     RRRQAVRGCRTCASCQQDIELINKQRGVK"
     gene            complement(16152..16775)
                     /locus_tag="ACHLAG_001265"
     CDS             complement(16152..16775)
                     /locus_tag="ACHLAG_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFP0654530.1"
                     /translation="MTTQLIPVFEGTISNEPTLLVNARDLHGFLEVGKDFSNWIRAKL
                     NEYGFVENLDYILFSPNLAKTPGRRRKDYHLTLDTAKELAMVERNEKGRQIRRYFIEC
                     EKKLRNIQPVQTEQQFTAEDIILLCYVQLWMEKAQQLSKQLYPVMRELNSDYYGKLFD
                     LAYETRHITNRTRDTLLREAAKLDPANYMVHRARSMLAQLRARQFEF"
     gene            complement(17043..17312)
                     /locus_tag="ACHLAG_001266"
                     /pseudo
     CDS             complement(17043..17312)
                     /locus_tag="ACHLAG_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
     gene            complement(17727..17831)
                     /locus_tag="ACHLAG_001267"
                     /pseudo
     CDS             complement(17727..17831)
                     /locus_tag="ACHLAG_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(17842..20265)
                     /gene="ynfF"
                     /locus_tag="ACHLAG_001268"
     CDS             complement(17842..20265)
                     /gene="ynfF"
                     /locus_tag="ACHLAG_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFP0654531.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDLFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(20326..21360)
                     /locus_tag="ACHLAG_001269"
                     /pseudo
     CDS             complement(20326..21360)
                     /locus_tag="ACHLAG_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
     gene            21418..23001
                     /locus_tag="ACHLAG_001270"
     CDS             21418..23001
                     /locus_tag="ACHLAG_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP0654532.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYLYQQVCTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTIPFLSVSTRSTDRRVATMRQRELAATAKSFMLDRPD
                     VSLQELTDHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDASGLIKLIDDNNHTDYSTIGDSTSILKNEQGDRPAVFTQE
                     RQPSVVFSSLVSSLLAEKVQTLKSSSYKDLSSSLNTVSRFLPEDMDLMSRSEWLAVRD
                     SMLAAEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVESEYQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLDNGL
                     TCIDINEDGDGKSVKNKHSVRLVPLTDGACGFDLKSFLEWVDTQPDDGGPLFGMTPSA
                     YSSWFNSRVLTEALGDAENVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITYDL
                     YGKGHAVGRLAEVLKTALL"
     gene            23495..23863
                     /locus_tag="ACHLAG_001271"
     CDS             23495..23863
                     /locus_tag="ACHLAG_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654533.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            24524..25282
                     /locus_tag="ACHLAG_001272"
     CDS             24524..25282
                     /locus_tag="ACHLAG_001272"
                     /inference="COORDINATES: protein motif:HMM:NF016283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFP0654534.1"
                     /translation="MSTQPLMKHEVVLPVIVDGYHWACNQDRMWNLNELHKELELPSA
                     KEPGQWRSSVRTLLDQDANLHVAHGGSTPGTWATEEAAIAYAMWVSPDFYLKVIRAFI
                     ALRNDKVSEAKALAQDSKELKGLKPIARNWLEKLDNPKQGHTLTECLKNLDFRLGTKL
                     VSAKALNAVLKSGRIANPFYSRKLDGRGQPVFDISSGGWMTSFNPKGLENGYYRLQDN
                     HYNGQEGLKVLTKGYEWLKSNKVELVRLCAKGGL"
     gene            25548..26039
                     /locus_tag="ACHLAG_001273"
     CDS             25548..26039
                     /locus_tag="ACHLAG_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654535.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSRESMELLEALAEAGVAIGNFFSNDLMTAYGRRQQNKRAEAERIAKEQRIQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWL
                     DSL"
     gene            26121..27110
                     /locus_tag="ACHLAG_001274"
     CDS             26121..27110
                     /locus_tag="ACHLAG_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654536.1"
                     /translation="MMTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLTDRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(27986..29398)
                     /locus_tag="ACHLAG_001275"
                     /pseudo
     CDS             complement(27986..29398)
                     /locus_tag="ACHLAG_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
     gene            complement(29597..29902)
                     /gene="ynfD"
                     /locus_tag="ACHLAG_001276"
     CDS             complement(29597..29902)
                     /gene="ynfD"
                     /locus_tag="ACHLAG_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFP0654537.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            30010..30720
                     /gene="ynfC"
                     /locus_tag="ACHLAG_001277"
     CDS             30010..30720
                     /gene="ynfC"
                     /locus_tag="ACHLAG_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFP0654538.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(30723..31283)
                     /gene="speG"
                     /locus_tag="ACHLAG_001278"
     CDS             complement(30723..31283)
                     /gene="speG"
                     /locus_tag="ACHLAG_001278"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFP0654539.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(31318..31659)
                     /gene="ynfB"
                     /locus_tag="ACHLAG_001279"
     CDS             complement(31318..31659)
                     /gene="ynfB"
                     /locus_tag="ACHLAG_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFP0654540.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            31794..32120
                     /gene="ynfA"
                     /locus_tag="ACHLAG_001280"
     CDS             31794..32120
                     /gene="ynfA"
                     /locus_tag="ACHLAG_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFP0654541.1"
                     /translation="MIKATLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            32326..33540
                     /gene="rspA"
                     /locus_tag="ACHLAG_001281"
     CDS             32326..33540
                     /gene="rspA"
                     /locus_tag="ACHLAG_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFP0654542.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTLAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            33552..34571
                     /gene="rspB"
                     /locus_tag="ACHLAG_001282"
     CDS             33552..34571
                     /gene="rspB"
                     /locus_tag="ACHLAG_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFP0654543.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGDGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADRAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(34759..36039)
                     /locus_tag="ACHLAG_001283"
     CDS             complement(34759..36039)
                     /locus_tag="ACHLAG_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFP0654544.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RSSVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRAIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKQKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNESMKN"
     gene            complement(36074..36325)
                     /locus_tag="ACHLAG_001284"
     CDS             complement(36074..36325)
                     /locus_tag="ACHLAG_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP0654545.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRCHLT"
     gene            complement(36398..38869)
                     /locus_tag="ACHLAG_001285"
     CDS             complement(36398..38869)
                     /locus_tag="ACHLAG_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP0654546.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RANQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSV
                     NQNQHESERNYQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCG
                     AVMGDATYQETFDEEYQVEVQEDDPEEMEGAEHPHKENTGGNQHHNSDNETGETADHP
                     IKVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGANFDNTILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPSQADS"
     gene            complement(38965..39153)
                     /locus_tag="ACHLAG_001286"
     CDS             complement(38965..39153)
                     /locus_tag="ACHLAG_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP0654547.1"
                     /translation="MTSAFALVMTVFLITGEPQNVITGIYDSKSSCIQVRDEQKIPGE
                     CLPLKKVSLNLNNEIPAG"
     gene            complement(39150..39338)
                     /gene="dicB"
                     /locus_tag="ACHLAG_001287"
     CDS             complement(39150..39338)
                     /gene="dicB"
                     /locus_tag="ACHLAG_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP0654548.1"
                     /translation="METSLPNVNTSEGCFDIGVLLSNREFTEDAIKMRKYEPYLLNDN
                     SILSRIALLELGIIGEQQ"
     gene            complement(39349..40203)
                     /locus_tag="ACHLAG_001288"
     CDS             complement(39349..40203)
                     /locus_tag="ACHLAG_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP0654549.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKAGAGRGNPMMTTAHNRAQAVFLC
                     VMHSHIQIMVGRAGQPKGWPVSVVTGISTPVRLTTLMVVESLGGELFKLTIEAAIMAT
                     IPALSHPDVTIENGRAVTTSIAIAEFFGKRHERVLDKIRNLDCSAKFTEHNFVSSEYT
                     DSTGRKLPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKELRQNNAQSPDKM
                     IHGDGRTLVIRLDEHGNIKFTETVPDGAMVCTLDTFQFYLEKQGWTLVNRSAIKNMTV
                     EQLLKIHC"
     gene            40514..40771
                     /locus_tag="ACHLAG_001289"
     CDS             40514..40771
                     /locus_tag="ACHLAG_001289"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654550.1"
                     /translation="MEETPAILTAAPFRGLPQPEAHDRIKFNDAVQRDELRRALSCYA
                     LTFQGNILSANCQCRILIRVRRTRSTSPVENYLITNLSFVG"
     gene            complement(40775..40993)
                     /gene="ydfC"
                     /locus_tag="ACHLAG_001290"
     CDS             complement(40775..40993)
                     /gene="ydfC"
                     /locus_tag="ACHLAG_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFP0654551.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANEARAKQAE
                     NLSALEQDITRLEKAAALSIARMITYPR"
     gene            complement(41023..41151)
                     /gene="ydfB"
                     /locus_tag="ACHLAG_001291"
     CDS             complement(41023..41151)
                     /gene="ydfB"
                     /locus_tag="ACHLAG_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFP0654552.1"
                     /translation="MDFDTIMEKAYEEYFEGLADGEEALSFNEFKQALSSSAKSNG"
     gene            complement(41153..41308)
                     /locus_tag="ACHLAG_001292"
     CDS             complement(41153..41308)
                     /locus_tag="ACHLAG_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP0654553.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
BASE COUNT        10125 a         9859 c        10768 g        10604 t
ORIGIN      
        1 ttcatttaaa atgtctaaaa agatgtatta ttaaaaagtt tagcgtgtta tctgaataca
       61 ggatatctta aatgaagagt atagcaacac tggttgtgtg tgcaatctcc gggattgcct
      121 gtgtaaattt atctgcacat gcagcagaag gaaaccatac aatttctctg gggtatgcgc
      181 actttcagtt tccgggactg aaggattttg taaaggatgc gactgctcat aacagggaga
      241 ctttcagtca tttcgtcaac agaaactact tttcttcatt gggcgaatat acagatggtc
      301 gggtcagtgg atatgaaggc aaggataaaa atccacaggg cattaatatc aggtatcgct
      361 acgagataac ggatgatttt ggcgttatca cctcttttac atggacgcgt tctctcacta
      421 actcacagac atttattgat gtgcagtcag ccgatcatac caggaagatt aagaatccgg
      481 cagcttctgc cagaacggat atcagggcga attactggag tctgttagcg gggccttcat
      541 ggcgggttaa tcagtacatg agcttatatg cgatggcagg gatgggcgtt gctaaagtta
      601 gcgctgacct gaaaattaag gacaatatta acagtagtgg cggattttct gaaagcaaca
      661 gcacgaaaaa aaactccctt gcgtgggctg caggtgcaca gtttaacctg aatgagagtg
      721 ttacactgga tgtggcttac gaaggttccg gctctggcga ctggcgcacg agtggaatta
      781 ctgctggcat tggcctgaaa ttctaacctg tatccggtaa ccgtttacta cccgctgtga
      841 tggcgggttt tttattgccc gtacagggca aaaaccgtaa attatgcgtg ggtgcctttc
      901 ggctgatggc tggagggtga acctgaaggc ctgatgtgga aaggccccga gtcaacttaa
      961 cgttaacccg aggccctaac acttcgtacc ttgacaagta gaaggttagc gcctctccac
     1021 tcaggaggca agcgctatgt cgcaaaaatc gcttatcacc gtcacaattt gtatgacggt
     1081 tatcttcact atctggatgt tgcacggttc gctgtgtgag ttccggctga atttgtgggg
     1141 agcggagttt gcggcgttct tacagtgtaa gcagtaggaa aaccgcgacg gggacgaaag
     1201 tccccgtcaa ctggttgctg aggttcagcc gatatggcac ccgtttcagg tgagagaatg
     1261 aacgataaaa ttctccggta tatgcagcgt gtggtgagaa attcccgcaa ccctgaattt
     1321 atgaatgaag ttaaagacgc ctgccttaaa aagcaggcgt tttgttttga ggcacctgat
     1381 ggctttctgg tgctgcgttc tgtgctcagt gctgatggta tcccttatgt tctggtgttg
     1441 ctgggcgtgt gtacggggag taacagcgtt gagcgttatc tgccggaggt gaagacatta
     1501 acccgtctgg ctggcggacg ttgggctgaa tttcatacgg caaggcgggg atttatccgg
     1561 ctgggaaaac gtctgggctt tgagcgaatg ccggatgatg aggatggctt catggtgttc
     1621 aggatagcgg tctgactgcc acagttttca tcatcgtgtt taaaccaaca ttgcaattca
     1681 cattctgacc ctgctccggc agggtttttt tattatccag ggggccatta tgggtggaag
     1741 taaaggtggt ggtgatacca aagtaaaacc aacagcagcg caaatagcac aggaagaagt
     1801 ggcctggaaa gggtggcagg attacaaaaa tatcctccgc ccggctgaag ataacttcat
     1861 ggaaaaggtc gatgacctga acagtgagca gcagtacgac aatatcgctg gcaccacaaa
     1921 tctggggtat cagaaacagt ttggcgaagc acggaaggag ctggcgggta atcttgctca
     1981 gtccggtgtt gacccgtcca gtggtcgctt taacgcggta atgaatgcga accagagtga
     2041 tcaggtaacc gggcagattg acacaaccac acgggggcag gtatcgcagg cagataagta
     2101 tgttgccggg ctacaggatg ttgcagctct cggttcaggt cagaaggcgg atgcgttaca
     2161 gagttttaac tcgctggcag acagcagtct ggcaaaagct aaatcggacg cacaggctgc
     2221 gtttacgaaa cagcaggggc gagcctctct tgttggcgct ggtttgggtg cggtaggtgc
     2281 atatgcgatg cataaggctg gtggtagcgg aggaagtggc ggtgctaaaa caccgggcac
     2341 cggcgctaat gccattcagc atcaggctca gaactggaga ctgtgattat ggagtacggt
     2401 aaatacgaaa cacttgcgag atacggttat accggagctg cccgtccaca gggtgactgg
     2461 cagacgtcag cagcgctgac acgccaacaa tacgacgact ggcgaaccag atatttgccc
     2521 cgcgtggcaa ggctggctga ccttggcgag aacaacagcc tgatgaatgc acagcttgcc
     2581 cgggtgggcg gccttgccac ttccagtctc cgtacagcgc agatggcgca ggataaccag
     2641 atggcgagat acggggtaag ccgcccggat aatcccgaca gtaatacgct ggggttacgt
     2701 aatgccctgg caattgctgg cgcgaaaaat ggtatccgtg aagccgaaca ggatcgccag
     2761 atgaacatac tgacgggggc ttctgcaccg gcaagacaga aactgagtgt tggcggacaa
     2821 ctggtggcag cgtaaggagg aaatatgggg tacggcttac tggatattgc gaatcagtca
     2881 cggcgtgagg cattacaggg aataagtgac gcagaccgac gacgtgaaga aattgaggct
     2941 gcgaacaaac agatggcggc gcaacagaaa gcgcagaaca agcagaatat cggtacgggc
     3001 attggtacgg gggcggctat tggcgcatcc gttggtggtc ctgttggtgc tgttgctgga
     3061 gcagtaattg gcggcattgc tggttctttg ttttaaggag tggtgaatga gcggatttgc
     3121 acaggggtta cttgccggat tcagcacggt tgaccaggca atgacccgtc gtaaggagct
     3181 tggtctgcgt gaagcacagc ttgcccggca acagaaaaat aacgagcgcg attttgagtt
     3241 tgcgcagtct cagtttgaac ataataaaaa cgttgatcag cggaactttg attacagagc
     3301 caaagttgac gaccgtaatt atgcactgaa ggaaagggag tttaacgcta accagaatta
     3361 ccggaatgcg tcgctgggta tggagcagca gcgactccgg atgcagaaat acaaccagcg
     3421 acggcttgag tataacgata tgctggcgcg cgatcagcct gtgatggcgg cgcttggaaa
     3481 ggctgtggat gctggcgatc gggatgcagc aatgcgtctt tacggccagt tgtcagaggg
     3541 taatccgctg aggctgatgg cgaatgatgg ctatgtagcg aaagcgggtc aggccgtgaa
     3601 caacctgcaa aaaatctttg atgacaagcc agacagggct atcgcttcac ttaatacgcc
     3661 ggagaatctt gatgtccttt ccggggtgtt tggcccggaa ctgcaacagc gtattggcat
     3721 gcccgattca accgggaaaa agacgataaa agaggccagg attggcagta tagtaccggc
     3781 acagcaggaa gggtacatac ttatcggcct tgatctcaca tacagtgatg gctctacagc
     3841 acataaacct gtaacagaat acggcagtgc gcaccctgat gatcaaaccg tgctggcgat
     3901 acccgttgat aaggctatcg ctcaggtcag ggatcgcagc aaatttgcag agatatcgaa
     3961 aaattatggt tattttatgc cgaagcagca gggactttct ctgaaagagc ttcagaaggg
     4021 ggccagcaac gtcgcggcgg acgcgatcaa gaatggcggt aatgcacagg ctgcggtgga
     4081 tgaatattat gctgcgactg gttcacaacc gcatcaacag aaaattcagc aacaaaaact
     4141 tcagcaacag gttatcaact gggcgggaga tgatcctgat aagctgtcat ttgccagaaa
     4201 tgtagcggcc cgtcagcctg aaatgctgga gcctcagaat cagaaattgc tggagaacgg
     4261 gtatgcgaat tttctccgta ttcaaaaggc caggggggaa caggccagag atgaaagtgc
     4321 ttcatctgca tctcagttta tccgtggact gaaacagaat tacgcccagt aattcacgat
     4381 attccattaa taccatttcc tgatgcccgg ccattgtgcc gggttttttt atggagtctg
     4441 tatggcctat tcagaggaac agcgtcctga ggcgcaactc ggtaaccaga atcgtaacag
     4501 cctgaacatt cagcaacccg gcgaaactga cagctatgaa gcatttttct ctgatccgaa
     4561 tcgctggaag gataacagta cgtcgttcag cctgggcgat gtattgccaa caatgggtaa
     4621 aggtttcgcc cagtccgtcc ggggaacagg ggaaatggcc cgtggactcg gtgatgcgat
     4681 gattcagagc ccggtaaaaa caggggcgcg tattttaaat gagttcagcc gtatggggct
     4741 gccgggtgtc gcaactgtgc aggatatttt tgccggtggc agcagggggg ctgatgaggt
     4801 catcgatacc ctgcctgatg gcaaaaacgc ggttactgat actgtcggta aaggtctgaa
     4861 ggcaaccggt aaggctgtca gtgatggtgc tgaagccact gatgaatggc tgaccggtaa
     4921 gatgtcgccg ggtgcagttc gtgcgctgaa tacgccgatg accgaaggct atgatgattc
     4981 tgcggtctgg gtggcgaagg gtgtaaacct gattggtgcg cttgtacctg atatggttgc
     5041 tggcggtgtg gctaaaaagg tgggtgatgt cacactgcga aaaatgctga ccgccgggct
     5101 ggagaaaaaa tacatcgcgg cagggatgca gccggaaaga gccacggcac tggcagcaga
     5161 agctgtcgat aaaaaaatgc cggatttatt ccaggcgggc ctgatcaccc attccacagc
     5221 cagtgcacag gggcagagtg caatggcggc agcagatgct gttcttaatg cggattactc
     5281 tgagctggcg cagtcaccga aatttcagca gacgtttttg tccattgacg ccgacccgca
     5341 gcacgcacag cttactgatc gccagaaaat ggatctggca aaagagcgtg ttgccgatga
     5401 ggtgcgcgcg cagctggcaa ccgatcctga attgctggct gtgaatgcca tggcggcaaa
     5461 actgggtgac gcacaactgt ttaatctggt gacacgaggc acagcgaaga ccgttaaaag
     5521 cggcattgtc agaaatgcca cggaacaggg ggcgattaat gcggcgcagg gcggctattc
     5581 acgctatcag gaaaacacgg cattgcgtga gaccgccgga atggatgtgt caccgtggga
     5641 gggcgtggct gacgcaacga tcgaaggtgc agcctttggt gctgcgatgg gggctccatc
     5701 cggtgcggtt gccggatatc gtggcagacg tcaggccgca gaagaagcag ccatgcgtga
     5761 tgctgaaacc gtgcagcagg acgacgcagc cccgcaacca gaatctgttg atccggtggc
     5821 gcagcatcgt gaatccatgc aggggatgaa tcgcgagcag cttctggagc agtatgctga
     5881 tgcggatatg gcaacagagg gggacgcatc cgcagctcat cgccgggaag ctgccagcca
     5941 gttgttgaat gaactggacg aacaggcgaa gcgacaggct gtgatgaatg agctgaaggc
     6001 gaagccgcgt tctgaactgc ttgaggaata ccgcagactc agccagaaag aggagcgcac
     6061 cgagactgaa gaacaacagt ttcaggcaat acgagaagtc attcgcccac aacaggaagt
     6121 gacgccggaa gcacagtcac agcctgaaaa tgcggaggat ggtaacggga gcatttaccc
     6181 gacggtgcgg ttccgggacc cgaatgaagt ccgcattgaa attaacggga atggtgcgtc
     6241 cagaccagcg gaacgcattg aaaaggtgcg cccggacaac cgttatttca cggatgagaa
     6301 aagcgccatg gggagtgatg ttttccgtaa tgccgccgcc accggcctga aaccgtccgt
     6361 agtgaagaaa ggcgagactc aatatgctgt tgaaatggat aatcctgcgt tctctgaaga
     6421 tgtggcaacg gaaaccatta acaccctggc tgacggagag cgtattgctg atgctgaccc
     6481 gatggagcag cccgcgttca tgcgtgaccc acgattccgt ggtttcacgg gggatgatac
     6541 ggaggtacag gcccgccttg cccgtggcaa cgtgccgacg gcagaggagc ttgtacgttc
     6601 acagatggct gaaggtgatg ccggtccgac agcacaggag ttaactgagc gtccacgcct
     6661 gcccgctccc ggcgatattc atcccggaca gggatatccg ttaccgggag aggtggcgcg
     6721 tacgccggat gagaatcagg caggacgtgg tggtcgtttt accacaaccg gtgaggttaa
     6781 gggccagagt ttccagaaag gacaagctct ggcaccggaa aacgccgctg gtcgccaggg
     6841 ggaaacactc gagggtgaaa tggttcgtcg tggtctgccg tcaccggatg cgcagaacgc
     6901 gacagcaccg gtacgtgaag ggctaccggc tccggacatt acacacaatg tccgtatgcc
     6961 tcagcctgat cagcttcccc gaactgttcg taactcattg cctgagctcg cacagcaggc
     7021 agaagtacgt cgacaggccg gaggaaatcg tgacatcccg cagcctgaga caatcgcacc
     7081 tgaatctgaa acaacagtcg ctactgacag ggaagctacc gtgcgcggag gtgaagtcag
     7141 gggcaaaaaa attgaagact ttggtgaaga aattaagggc gcggcaaaac accgttatgc
     7201 acagcttgct gaaacactgg gtaaaacgct ggaagacagg gattatgtca cgcaaccgct
     7261 gagcaaactg ttcccgaaac cggactacgc aaaactggcg agcgaagggg ctgatgctga
     7321 caccctggca atgatagcgc tgtatcgtag cgatattccg gtgaagacga aacacaatac
     7381 ggcaggctgg ggggagagcg taaaaaaagt acgacacagt gtatcggaaa tgctgaacgg
     7441 aacggtcagc gcgaaacgcc tcgcagaatg gatggaaggc agaatgccct cccgttacgc
     7501 ggacacctgg caactgttac gcactctgcc accctcacag atggacaagg cttctgctta
     7561 tcgggtggta tcgggtgtgt atcaggcggc aggagggaag cgttacgatc cgccacagaa
     7621 actttattca ctgcgcaata aggacaataa ggggagtaac ctctttttct cggaaagcag
     7681 ggatgaatta ctggcaaagg cgaaagtctg gtttgcagag caggaggaaa aatcacaggc
     7741 gaaaggtgat gaaaaaacag caccgtctcc ggatgacaaa atccgctttg acgtttaccg
     7801 gaatacccgc agtggcgata tttttatcgc ttacggtaaa aacaaaatgc gggtgagagg
     7861 tggctttaag tcagccagtg atgcgcgtaa gtacattgat ttacatcgtg atgagcttgt
     7921 tcgtcatgtg aaggagatgc gggagatttc gcgtgaggag cagcgcaacg ccaccaaccg
     7981 cgaccgtacc ggaccagaac gccgcaaggg ggatgtttca ccggagcagt tcagtgatgc
     8041 gtttggtttt cgtggtgtgc agtttggtaa ttatgtggaa agtccgcgtc gtcaggctga
     8101 tttgaaccgg gcttatgact cgctgcatga cctggctgac gtgctgaatg taccgacaaa
     8161 agcgctttct ctgaacggtc gtcttgggct tgcttttggt gcccgtggta agggtaaggc
     8221 ggcggcacac tatgagtcag gtgaggtggc aatcaacctg acaaaaggta acggaccggg
     8281 tgcgctggcg cacgaatggt tccattctct ggataattat tttggtcgtt atgacgtttc
     8341 cagtgacggg aaaattacgt caggtggcga ctttatgacg gaagcacagc gtgtcaggcg
     8401 catatttaaa gacggcaggt atgttgatgc tgaatatccg gtacgtcagg aggtttacga
     8461 cgcttttaaa ggtgtgattc aggccattaa aaacagtgac atgccgcgtc gttcagcgct
     8521 tcttgatgag gtgcgctcaa aaccgtactg gtcaacggat gttgaaatgg cggcacgtgc
     8581 ctttgagcgt tatgttcagg ataaggcgcg tatggctggc gtggagaatg attatctggt
     8641 caatatccgt aaggcacctg agcacaacac agataacacc tacgcttatc cgacgaatgc
     8701 ggaactggat ggcggtattc gtgaggcatt cgatcacctg ttccgcaccc tgaaaacccg
     8761 tgagacggac aagggcgttg cgttttattc ccgtaagggc atgacccgca cacctgaagg
     8821 taatctcatt tcggatgtta accgtagtgc ggaagccaaa ggcagcccgg tcccgcaggt
     8881 tgaagcggtt gcccgtggcg tgatgagcgg cattaaggac agtgacctga cggtccgtgt
     8941 ggtgaagtca cagaaagagg ctgaagcgct ggcgggtgaa tcgttcgacg gttacggcag
     9001 ggtgcacgca ttctatcgtc cggataaacg agaaattgtc ctggtggcgg ataacatccc
     9061 tgacgggcag accgtacgcg agaagttgcg tcacgagatt atccaccatg ccatggagca
     9121 tgttgtcacg ccagcggaat atcagacgat tatcaaaacc gtgctgaaaa cccgcgacag
     9181 tgataacgcc accatccgtg aagcctggcg taaggttgat gcgtcctatg gtaaggaatc
     9241 accggaagta caggcgggtg aatttctggc acatatggcg gagaaacagc cgaataaatt
     9301 cgtggcggca tgggagcgtg ttgttgccct ggtcaaaggg gtactgcgtc gtacggggtt
     9361 actgaagccg acggaactga acgatatcag acttgttcgc gagaccatcc gtacgttagg
     9421 ccagcgtgtg cgggaaggtt acacgccgcg tgaggatggc gtgggcgcat cgtctcagta
     9481 ctcccgtagt ggtaagcctg atccgttcaa agtgccggaa ggtgagggtg aacgttatcg
     9541 cgatgacctt gccagaatga tgaaatctct gcgtaccaca gatttaacgg taaacatcgg
     9601 gcgtacgccg ccggtattgc gtcaccttgg tgcaccggat ttgccgctgg ttatttcccg
     9661 cgatactgtg cggaaggcca ccaatggtgt gaaacatgtg gtgccgatgg atgttatcga
     9721 gagactaccg gaactgatgc acgatccgga tgcaatttac cgctcagcga cagaaagaaa
     9781 tgcggttgtg atgctgcttg atgccgtgga taaaaatggt gatccggtag tgtctgcggt
     9841 gcatatgaag gcaacccaaa aacttctgga agttaaccgt attgcttctg tttacggaac
     9901 ggaaaacggg aaaaaactga agagtatgga aacggctggc ttaacattat acaggagaga
     9961 aaaactaaac cctgatggtt ctctgtacag agggctccaa ttgcccaaag atgagcactc
    10021 ccgtcagggt tctgcggata aaatactcta tcctgaagat attcgcaagg ggccgtatta
    10081 ctcccgtacc agcagtctga caccggaaga gacaattgca tcgcgttttg tgcgccagat
    10141 gcaggataaa ttccaggtgc tgaaagctgt tcaggagaat atccgtaaaa ctggtggaaa
    10201 aatagacgac agtaacaacg cttatatggc ggaagaactc ttccacggga aggcggaaaa
    10261 cgacctgaac gtgatgaagg agcgctacgt tcagccactg gctaaattac tggcggacta
    10321 caaaattgcg caggccgatc tggatgagta cctctacgcc cgtcacgcgc cggaacgtaa
    10381 cgcgcatatc gcgaaaatca acccgaaaat gccggacggc ggttcgggga tgaccaacgc
    10441 ggaggcggcg gaaatcatgc agcgtgtacg taacagcggc aaacaggcac agtatgaccg
    10501 tctggcaggg attgttgacg atatgctggc ccgtcgccgt gagcttatcc gtgaggcagg
    10561 actggaagag agcggcgtgg tggatgcctg gcagaaagcc taccgttact acgttcccct
    10621 gaaagggcag gatgttgacg gtgtggtgtc actgccacgt acaggcaagg gattcaccat
    10681 cggcgggcgt gaaagtaagc aggccatggg gcgcgcatcc cgcgcacagt ctccttccac
    10741 tcaggcgata caggatttga gcgaatcact gatccgcaat cgcaaaaatg aagtgggtaa
    10801 cgccttcctg aaactggtac aggataatcc cgacaaggat tactggcagg tattcaccga
    10861 tgacagaccg gataccatgc ggacgattgc agagcgcaag gacaaggaaa ctggtgaaac
    10921 cattcgcgaa gttgtcgaac gccctgtacc gatggcaatg atggcagacc ggtacttcac
    10981 caccaaaaag aacggcaaaa cgtactacat caaactccat gatccgcgcc tgatgcgtgc
    11041 gatgaagaat atgggaccgg aaaccagtaa cgccgtaatc cgcacgctgg ggaaagttaa
    11101 ccgcttcctg gcaacggtga acacgtcgta taacccggaa ttcctggtca gtaacttcat
    11161 ccgtgacgta cagacggcgg tgatgaacct gaaggcggag cagggaagga gcgacggcaa
    11221 actgaaaggg ctggataact tatccgcact ggctgtggtg aaagacagtc ggtctgccat
    11281 gtctgccgta tacgccagtc tgcgtggtaa aaccctcacg ggcaacggtg cgcagtggca
    11341 gaaggtgtgg aaagagtttg ttgaggacgg aggtaaaacc ggctggttta acatgggtga
    11401 ccttgaaggc cagcagaagg aaatggatcg ccttgtatcg ctggcgaagg gaggatggaa
    11461 aggccagagt atcggtgcat ggaattcgtt ccttaacctt gtcgaggatg ccaacggggc
    11521 ggttgaaaac gctctgcgtc tttctgccta taaacacgcc cgtgatgccg gtttgtcacg
    11581 ccagcaggcg gcgtctcttg ccaaaaacat gacggtgaac tttaaccgtc gtggtgagca
    11641 gggggcgctg atgaactcgc tgtacatgtt cgccaacgcc agtattcagg gaacggcaaa
    11701 tctggtgaga acgctcggac atcttaatgg tgagggacct ttactggagc gccttcgctg
    11761 gaagaatctg aatgttccgc agaaaatcgc gcttgccgct gtgggagcgg gttatctgct
    11821 tggctcgctt aaccgcagcg tggcgggtga ggatgatgac ggggttaact ggtatgacaa
    11881 agtaccgtct catgtgaaag agcgtaacct cgtcattatg aaatcggtgt tcgggggcaa
    11941 ggccggagag tactggagta ttcctctgcc ttacgggtac aacgttttct tcctgcttgg
    12001 tcacacagcc gaaggggtgg cagcgggcga cctgaccgct tcacgtgctg ccggtaatgt
    12061 tgttggtggt gtgcttgggg cattcagtcc tgtgggcagt gaaacgtcgg aaacactgtc
    12121 cggggcattg ctgaaaaatg cagcgccgac cattctgcgt ccgtttgcga accttgccat
    12181 gaatgaaaac ttcatggggg cgcagattta ccaggagaac atgccgtttg gtacaccaaa
    12241 acctgacagc cagctgggaa gacgttcaac gccagaagcg tacaaggcgt ttgcatcctg
    12301 gctgaatgcg ttctcaggtg gcagccagta ccgtcccggc gcggtggata tcacaccgga
    12361 atcgctgaaa ttctggattg actatatctc cggagggaca gggcgcttca tttccaaaac
    12421 cacggatgcg gcggtgaaat cgctgaatgg tattgatata ccggaacagc aggtgccctt
    12481 cctggggaaa atttcggggg aggtgatgcc gtatgcagac cagcagaaga tgtacgaccg
    12541 gatgacagag attgcgcagt atcacgcaga gctgaagagt ctgaccggtg cagaaagaac
    12601 ggcgttcatt gacgagaaca acggaaaatt gtcgatgaac gggcttatgc aggatacccg
    12661 gaagagactg aaggatttgc gtaaacagcg tgatgccatt tatgccgaca gttctctcag
    12721 tctggcgcag caggcggcga tagtgaaatc ggtagagcgg gatatgaagg ttgccgtgga
    12781 tcgctttaac cgcgagtaca acaaaaaagt gggagtggat taacagaaat ggccccgtac
    12841 ggaagtgcgg ggctgattaa ggaataaaca cacattaacc tgtaataacc ggagctatta
    12901 acatatagtc agaaagagta tttcatgtga gacagagagc cgatttatgt ttaatgaaga
    12961 aaaagttgcg caaatggcag cgtatttgct gaaaaagcat ggcggatcta tgcgtttcat
    13021 taagctgatg aagctgatgt atctctctga ccgcaaagca atggagtctt taaccgggaa
    13081 ggggagaggt ttgaatgctc ccccacaatt ccccggcatc aaatctccat gcaggtgaac
    13141 tattttaccc ccagcggcaa atcgcaaaaa caatcagtgc gatcgaaatg gcagccacta
    13201 caattgcaaa tgcttcaggc taggtcattg gcttacctcc ttcggcggtt ctggtagcgg
    13261 catccagtgg gttacttctt tgagatgcag gtcttcgcca tcaccgtcat cccaagtggg
    13321 attgccatca ttaaaccagt cgccatatac gccgacctga gtgttgggga tgcttggtgg
    13381 gtagttgttt ttaaagtcag ctgctaacac atagtattgt cgctctccca tttctggcat
    13441 tcgatcacta cagcttatcc aaccatccgg agttaccgga actggcggaa cggctgtttg
    13501 ctctcgaacg tcattagtcg ctatcggttc tgctgccaac tgactggcat atttgttaat
    13561 ggtaacgata agctcttgct cagcctcatc cagacaatca ccgatacctc gcctgtcacc
    13621 gtcaaaatca tcgaaatcgg cacgaatctt ggcaaccttc tggattgcgg acaacacctc
    13681 actaggaatt accggatagt tggttgacgt ttccgcgatt tcccgaaaat tattggttga
    13741 cgaattcttg ttttcccgaa agtttccgga ctgaagcatg gcggcgcggc aggcattcca
    13801 gcctcttacc tctgcaatag cggcaaccgc atcaaccgcg tacatgctaa gaggattagg
    13861 cattggtttt tcttccggta ctactggtgc tggaggggcg gcgtaaatgc cctctatcac
    13921 taaatgtttg cgctcaaaat catctggctc tcgatgatat acgtaactcc aatcaccaag
    13981 gttatcattg cgcctgcaac ggaaacctat cggctcggct tccagcgctg ccagagcaat
    14041 tcgtgccagt tcttccgctt cttctgctgg cagtacaacg ttgctaccag gtccgtatgt
    14101 ttcgcgccac tgcttgattg tcagtagtcg ctctttggta atagtgatca tgccgcgttt
    14161 ccttctttct tattaacaat tacaccgtca tatatttcat taaggtgccc tctcaactcc
    14221 atgcgcctta atgcagataa catgtaatcg cattcaacct gcttattccc aataaaaggt
    14281 ttatcttcag ggttacccca acagcaattc cccttgggcc atccatgtac tttccgtact
    14341 cttccgttaa caacgtgaag taatccccag ccaggtggta aatcctcaat tgaaataatt
    14401 cccggctcac taataaagaa tcgccagtcg cccattccaa gagacggatt tttacgaaaa
    14461 cgcttttttc tatctgccaa caagtcagca cgagaacatt tcgcctctat caggcatgat
    14521 gctgaatttc tgaatcccat agcatctggc tgttctccgg tactggttac agctataaag
    14581 cggtcatgaa aacaaacctt gaacccgttg cgcttaagga acttgtacgc aatctgacag
    14641 agttcgcggt gtgttaacgc catatcactc tcctttgatg cgaatgccag caagccagtt
    14701 tcttatgccg atatattcag cgttccggaa accgcttttt acatatataa atggcaagcg
    14761 aagattgtga ccattgactg ccaggtagtc tttacaaccc tgttcggtga aacagcaggt
    14821 aacgaattca tcaatatctt tcacagcaac gcgccgccat ttttctggtg gctctcgaaa
    14881 gttttcatga agtagctcga gacgacgact atggcgttta ttggcttcat tgccatcttc
    14941 gtcaacccag acaatccggt catggtcata atcagcatca acaacgattt cgcgcttttg
    15001 atacacacaa aacatgggat ctgacgttat tcgattgtcc tgtgttcgaa tattttcacc
    15061 gatgatgcca aacgaatctg gtgcagattt tgtctgcatc tcttcgatac gttcagccat
    15121 cgcagcacac tcttcaaagt tgcttaatgc ttttcgctcc cattcggcgc attgtttttc
    15181 cagttctgct atgcgcttac ttccatccgc gattactccc tcgtaatatt cacgctgctc
    15241 gttgagtttt gattttgctg tttcaagctc aacgcgcagc ttccctaccg taagcgcaat
    15301 atcctcgttc tcctggtcgc ggcgtttgat gtattgctgg tttctttccc gttcatccag
    15361 cagtgccagc acggtagccg gattggctgc ggcgatgaat tcagcattgg cctgctgttc
    15421 catttggaaa tcttcatcga aaccgctttc aggatgtgct ccttcaattc tgcaaatggg
    15481 aatatatcca gcagcctcgc gatgaattag tgcatcatca ccatcaaatc ggttctctcc
    15541 atattcgagc gaccactcac cacgcgttgc tttttctgcc ttttcacgca gtacctgata
    15601 gttaatctcg ctcatttttc tcttcgctcc ggtatacaag aattacaacg tcacctctgc
    15661 taattacgcg agctggatct cctggctcca tgctgtcaat cccgaaggct tcggaaaacg
    15721 cattcattgc cttctggcgt tgatcctgct tacggcattt attccatttt ttcagtaaca
    15781 acagcgatag ccaccgcccg gcgcagaaca taatgtaaaa ataaccaaga agcgccagac
    15841 ctgtgttgag ggccgtatcg atcgttatag tggtgtctat gctcacttca cacctctctg
    15901 tttgttgata agttcaatat cctgctggca actggcgcaa gttcggcatc cgcgaacagc
    15961 ctggcgtcgc cgctcatcta tcggatcgcc acactcgcaa caatgagtag cagatggggc
    16021 attactttcg gatttgtatt tttgcaggga gagattgcgc tgcaattctt cgatttcagc
    16081 ggcattgtcg atgatatctg ccattttcct ttccttcagg catgaaaaaa ggagccgaag
    16141 ctcctttggt atcagaattc aaattgtctt gctcttagtt gcgctagcat acttctggcc
    16201 ctatgcacca tatagttggc gggatcaagt tttgctgctt cgcggagcaa tgtgtcgcga
    16261 gtccggttgg ttatatgacg agtctcgtag gccagatcaa acagtttccc gtagtaatca
    16321 gaattcagtt ctctcatgac ggggtatagc tgcttactga gttgttgtgc tttttccatc
    16381 cagagttgaa cgtagcagag caggatgatg tcttctgctg tgaattgctg ctcagtttgt
    16441 actggctgaa tgttgcgaag tttcttttcg cactcgatga agtatcggcg tatctggcgg
    16501 cctttttcgt tacgttcaac catcgcaagc tctttggctg tgtcgagggt gaggtggtaa
    16561 tccttgcggc ggcggcctgg agttttcgcc aaatttggcg aaaatagaat gtagtctaaa
    16621 ttttctacga atccatactc gtttagcttg gcccgtatcc agttagaaaa atctttacct
    16681 acctctaaaa atccatgcaa atcacgcgcg ttcaccagaa gcgtaggttc atttgagatt
    16741 gtaccttcaa acacagggat gagttgagtg gtcatgatga cctcctttgg cttttttcga
    16801 gaaatgccac cacaaaatgt ggtgccggga ggctcgaaac ggcccaaaag ataccgcgga
    16861 cttattcccc ttgcgggtgt tgtattcgtc gccctcccga cattgatcgg ggatgtgacc
    16921 gcacactgtg ctatcactga ataacaggca taaaaaatcc aacactgacg gggttggttt
    16981 aatccgcttt tgggaggttt tcgaggcctc ggcgcggagt atagtcagta ttggacgcag
    17041 tcgtcaacca tcgccgtttc tttggctgtg tcgagggtga ggtggtaatc cttgcggttg
    17101 tgaccgcctc taccagatgt ttgctttccc aaattggaaa gcaaaatata gtcttgattt
    17161 tcaatgaatt cgtattctga aatgcgattt gtaatccatg ccgcaaacac ctttttcacg
    17221 cctaaaaaag catgcagatc gcgggcattg cagagtaggg ctgtttcgtt gtcgatagtg
    17281 ccgttgaata cggggatgag ttgattagcc ataattatga ctcctgacgt tttagtattg
    17341 actgccacct tagtggggtg gcgggcttca actaccgcgt cagacggcgg agcgtatttc
    17401 ccgaaagggt attgtattag gctctctcga cccgccattg atatggcgat acctgtaaac
    17461 aggcataaaa aagccgcaaa gctatcgggt gcggttgacc gctgacgttg tagtgcagtc
    17521 agtatgcgat agctctggca gatttgtcaa atcgtgcagt aacatccttt tcttccttgc
    17581 catttctcaa tgatgacaaa gggtggattc ggattggtat tgggacaaaa gtgagacaca
    17641 caaagctttg catcggctta caaagctttg catgtttttc aatgttggga cgtgtgagcg
    17701 cagaaatgac gggctatcta attgatttta aacgatacgt aaccaacttt aaaatctttg
    17761 cacgccagtt cgcaggtttt acagccagta cagcggctgg aatcgataaa aaatccatat
    17821 tgtgtggtca tgggctactc cttaaacctt ttcgatctgg acaagattgc tgtgcgacgg
    17881 gtttcccttt gccagcggtg aagggcggtg agaggtcaga atattgatac tgccgccgtg
    17941 atcgacccgg tcaccaaaca tatccgcttt aagccacgca ccttgcccga tggcggtaac
    18001 gccaggcaga atacgcggag tcacttttgc ggcaatcagc atttctccat tattgttaaa
    18061 tacccgcacg gtatcgccat gacggatacc gcgtgcctga gcatcaatgg ggttgatcca
    18121 cacctcttgt gggcaggcct gctgtaacac atcaatattg ccgtagctgg agtgggtacg
    18181 cgctttgtag tggaagcccg ttaactgcag tggataggtt ttccgcaggg gatcgtccca
    18241 gccatcaaaa cctggggtat acgcaggaag gggatgaata atttcatctt ttttcaattc
    18301 ccaggtatct gcaatcttcg ccagtcgttc agaataaatt tcgattttcc ccgaaggtgt
    18361 tttcaacggg tttgcctgtg gatcttcacg gaatgcgcgg aaagcgacgt agtgttcttc
    18421 cgggcatttt ttcttaaaga tcccggtcgt tttcatctcc tcgtagtcgg gcatctcagg
    18481 gttacgttcc ttcgttttcg catggagata tttgatccat tcatgctgac tgcgaccttc
    18541 agtaaaggtt tgataaacgt ctggtcctaa gcgtttggcg acttcactca gcatccagta
    18601 gatgggtttg cgttcaaatt ttgctgaggt tgcgggttgg gcgaggatca catagcccat
    18661 attccctgca gattcatgag agataaggtc ttcttgctct gttggcatca ggtcgggcaa
    18721 caggatatcg caatacttag ccgaggccgt catgaagagg tcaatgccaa caatcatctc
    18781 gcacttgctg tcatcctgaa gcacctcatg ggtgtgattg atgtcgccat gttgattgat
    18841 caatgtgtta ctggcgtagc accataaaaa cttgatgggg acatccagtt tttcttttcc
    18901 acgaacacca tcacgggtcg cggtcatttc cgtaccatgg tcgatggcat ctgtccatgt
    18961 aaagacggaa atctgcgttt taacaggatt ctcaagcatc gggaaccatt ctacccccag
    19021 atcccagcta ccttcgcgta cacctgagtt gccgccgttt atgccgacgt taccggtgag
    19081 aacggaaagc atggcaatag cgcgggacgt ttgctcgccg ttggaatgtc gttgtggccc
    19141 ccaaccctga caaatataag caggttttgc tgaaccgatc tctcgtgcca actggataat
    19201 tttttctgcc gggatgctgg tgatttttgc tgcccattcc ggcgttttag ctatgccgtc
    19261 aggcccttcg cccagaatat aggctttata atgcgcgtta cgtggtgcgt tggcgggcag
    19321 cgttttttca tcgtaaccaa cacaatattt gtcgagaaat ggctgatcga ccatgttttc
    19381 agtaatcagt acccaggcaa tcgcacaggc cagtgcgcca tcggtgccag ggcgaatggg
    19441 cagccattca tcttcacgcc cggcagcagt gtcgttataa cgtggatcga tgacgatcat
    19501 gcgtgcgttt gaacgttcgc gggcttgctc gacgtagtaa gtgacaccac cgccgctcat
    19561 ccgcgtttct gccgggttat ttccgaacat aacgaccagt ttcgtattgg cgatatcatc
    19621 cgggctgttg ccatcattgg caccgaacat ataactcatt gcggcactga tctgtgcggt
    19681 actgtagctg ccatagcgac tgagaaaacc accgcaagag ttcatcagac ggtacgggac
    19741 gtttgagttg gtgatgtttc cgccatctac gcctgttccg tacaggacat gtacagcctc
    19801 attgccgtaa tctttcagga tccgccgaag attatcactg atggtatcca gggcttcgtc
    19861 ccaacttatc cgttcaaatt taccttcacc gcgcttgccg acgcgcttca tgggatattt
    19921 caacctatca ggatgattca tccgtcggcg gatagagcgc ccgcgtaaac acgctcgaac
    19981 ctgatggtta ccgtagacgt cgtcacctgt cgtatcagac tccacccagt acacggtgtc
    20041 atctttcaca tgcaaacgta acagacagcg gctcccgcag ttaacggtgc aggaactcca
    20101 gaccgctttc tcttctaccg gagcctctgc cgcccggacc atttgggaaa atggcagagt
    20161 gaaagcactg cttgccagcg caagactgcc aagtgcggag gttttcatca gacttctacg
    20221 gctgatttca gccttcatga gcgcctctgt ggtatggatt ttcatcatta ctcacttatt
    20281 gcttttcaaa caaaatgtca tgccagaatt tatgattgtc gtgggttata ttttttcgat
    20341 ctcgaccaga ttagtgtgct gcgggtttcc cttcgccagt ggtgaagggc gcagagtggt
    20401 tagcgtattc acacagccgc catggtcgat tttatcgcca gacatattgg cctcgtgcca
    20461 ggctccctgg cccatagcgc taactccagg gagaatacgt ggtgttactt tggctggtag
    20521 ccgaacttcg ccacgatggt taaacacccg caccatatcg ccgttggcaa tcccacgttt
    20581 ctgcgcatct atagggttga tccacacctc ctgacggcag gcagccttca ggagatcaat
    20641 attgccgtag gtcgagtgag tacgggattt gtaatggaaa ccaaacagtt gcagtgggaa
    20701 ggttctacgt tcaggggagt cccagccttc aaaggtagag gcataaactg gcagtgggct
    20761 tatcacttca tctttttcca gttcccaggt acgggcaatt tccgccagct tgctggaata
    20821 aatttcaatc ttaccggaag gtgttttaag tggatttgcc tcggggtcgt cacgaaatgc
    20881 tttgtaggcg acaaaatgac cattgggatc tttacgctta tagataccca tttttttcag
    20941 ttcgtcgtaa gacggtaacg ccggatcttt ggcaagcatt ttggcgtaca gatgttgtaa
    21001 ccattgttcc tgcgtgcgac cttcagtgaa cttttgatag acgtcaggtc caagacgttt
    21061 cgcgacttca ctcaggatcc agtaaatcgg tttgcgttcg aatttttcgc tggtgacagg
    21121 ctggaggaaa atgagatatc ccatgttacc ggcgtagtcg ttaggaataa tatcttcctg
    21181 ctcaacggtc atcaggtctg gcagcagaat gtcggcatat tttgccgatg aggtcataaa
    21241 gttttcgatg accacaatca tttcgcattt cgattcgtcc tgcagaattt catgcgtttt
    21301 gttgatgtca gaatgctgat taacgagggt atttcccgcg tagttccaga tggactccat
    21361 tgtcagacgg atggatggac ttgaagtagt tgactcaatg ctacttaagg tcctgatatg
    21421 actgacatta accatgataa tactgtatat ccatccagtt ctttctctgg gtctccctat
    21481 ctgtatcagc aagtctgcac ctcaagtcct gacacaaggc tgacacaaat caatctactc
    21541 agcgtagcta agcctcagtt agtccgtaga gccaatggtc gatatactat ccgcttcaga
    21601 ctcaagggac agaccatccc gtttctttca gtatcgaccc gaagcacaga caggagagta
    21661 gccacaatgc gtcagagaga gcttgcagcc acagctaagt ccttcatgtt ggataggcct
    21721 gatgtctcgc tacaggagct tacggaccac ctccggtcta tggctgagca gttcctgaca
    21781 gacgccagtg atgactactg gaatggactt gaggtcgcta ctctggtgga cgagaagtcc
    21841 aacctgaagg agctagccgc tacgcaagcc ttgagtctgg accagcagaa gggcataagg
    21901 ttagcgcttg aagtcctgac cgcagcacag cagagggtag acactggtga tgcctctggt
    21961 ctgattaagc tgattgatga taataaccat actgattact caactatagg cgactctacg
    22021 tcaattctga agaatgagca gggggataga ccagcagttt tcacacagga gcgtcagcca
    22081 tctgtggttt tttcaagcct agtgtcctcg ctgctggctg agaaggtcca gaccctgaag
    22141 tcctccagct acaaggacct gtcgtcctca ctgaataccg tctctcggtt cttacctgaa
    22201 gacatggacc tgatgtcccg ctctgagtgg ctggctgtca gggactccat gctggctgct
    22261 gaggtgagac catcgacaat caacaagctg ctgactaagg cgaagatgtg tcttgattat
    22321 ggcctgatga atgggcaact tgagggccgc aaccctattg aacggatgaa gctgaccaag
    22381 gatatcgact ccaagcgcag agcgttcaca gacgaggagc tggagagact tctggtccgt
    22441 gttgagtctg agtatcagtt cacacgacac acggctcaca cgacctctga ggcccgtaga
    22501 tgggcgtctc tggtgtctgt ggtcactggc gcgaggtctg ctgaagtctg ccacctgact
    22561 aaacgtgaca ttgtcacctt agacaacggt ttaacctgca tcgacatcaa tgaagatggg
    22621 gacggtaagt ctgtgaagaa caaacattca gtccgacttg taccgctgac cgatggggcc
    22681 tgtgggttcg acctgaagtc cttccttgaa tgggtagaca cgcagcctga cgacggcggt
    22741 cctctcttcg ggatgactcc gagcgcctac tctagctggt ttaactctag ggtcctgact
    22801 gaggcactgg gagacgctga gaacgtttct ctccatagtc tgaggcattg gctggcgacc
    22861 cgaatgaaag agcgtggagt caatctggtg gacgctcagg gcatcttagg ccatagcagt
    22921 cagtcaatta cgtatgacct gtacgggaaa ggacacgctg ttggtaggct ggctgaggtg
    22981 ctgaagacag ctttacttta agtaggaaga cccaaggtgg agaccatata cggcttgatg
    23041 gttgagacct tgggtcactg aagtctgact gaagttaggg agggtgataa taatcataat
    23101 ccctcctgac tataggccca ctggttcgcc ttcggctgtc agactctaag tctgtatgtg
    23161 gtcttattta aactggttcc ctaactccag tactccaaat tcctatggtc tcctcttgac
    23221 attcctgttt aactgtgagt cgcctctggt ctctctttat atatacttcc ttaattcctg
    23281 aggcgtcttt agtccttgac atccagtcca gcctgtgagt cacatccggt cctccctctt
    23341 tatatatact tccttaattc ctagggtcgc cttaatcctt gacatccagt ccagcctgtg
    23401 agtcacatcc tgtcgcgcta ctgtatgcat caccagtgat ggacttgatt atcactatag
    23461 gggaaccaac taattcaact acaggagata ctctatggca cgaccaatcg caactcacga
    23521 caacacgttc accaaagcct acttgcagca gcactgcggg gacctgctgt cattcgatgg
    23581 tcagggcgac ctgagtggtt ggctggatga tgtccttact ggtgctggtc gcctgaacga
    23641 atcaatggca agcaatacca agcctgtcag cccatacttg attctgaccc aactactaac
    23701 gcacgacaca ttaactgtaa gcgctgtaca ggagtccctg agccgtaagc gtgttgcctt
    23761 gggtgaacct atggtttcaa ctcgctacgc tcgctacgtc tatgctaccg tcgtcagcgc
    23821 cagtaaatcc gtccagtacc acgccagtaa agctggttca tgattacgga ccctaaacta
    23881 tcactatagg gagaccagcc tccgcctctg agtctgatgc gtcagaacat gcgagcatct
    23941 ggtcaaccga cattaagtat ctctcctaag taatcaaggc atgtgaccgc ctctgtcaga
    24001 cctcgtgtat cgacctccag ctcgttagcg ttctcgtgtc ttcattatga tttgggtcta
    24061 tcaccgctca ccatctggaa gtccttacag ctcgtcctga catgcggtct cacctttata
    24121 tggctggaag agacctcctc tgtcagacgc tatctaacca acccagaaaa cgggtttgtt
    24181 aaaaagatta acatgggtct ccaccttcat gaggaccgct ccggaaattt ccggaccgtg
    24241 aacgggtggc tctatcacag gtcgcgaaaa tggaaacctg cttaacgtcc ccaaaactga
    24301 gcagtcacca ttcttaacgg gtccaaattt gggcgcgttg gcgagaagtc taaccaaccc
    24361 agaaaacggg tttgttaaaa aagttaacat ggtcttcaca cgacctcatt acctcaagtc
    24421 tttctgtatg tctcctgttg cctgtgagtc tccctatcgt tgggggcttg agtaggcttt
    24481 cagggtgtga cttcaggagg cataacaaaa ggagtacaac gatatgtcaa ctcaaccctt
    24541 aatgaagcat gaagtagttc ttcctgtcat cgttgacggc tatcactggg cctgtaatca
    24601 ggaccgtatg tggaacctga acgagctaca taaagagctt gagcttccat ctgctaagga
    24661 gccgggtcag tggcgttcta gcgtcaggac gcttcttgac caagatgcaa atctgcatgt
    24721 tgctcatggt ggttcgactc cggggacatg ggctactgaa gaggcggcta ttgcctatgc
    24781 gatgtgggtc tcacctgatt tctaccttaa ggtcatccgt gctttcatcg ctttacgtaa
    24841 cgacaaagtg tcggaagcta aggcgctggc gcaggactcc aaggaactta agggtctcaa
    24901 gcctatagcc cgtaactggc tggaaaagct ggataacccg aagcagggcc acaccctgac
    24961 agagtgtctt aagaatctcg acttcaggtt aggtacaaag ctggtatcag ccaaggctct
    25021 taatgccgta ttgaagtcag ggcgtatagc taatcccttc tatagtcgca agctggacgg
    25081 tcgaggacag cctgtatttg acatctcgtc aggtggctgg atgaccagct tcaatcctaa
    25141 gggtctggag aacggctatt accgtcttca ggataaccac tataacggtc aggaggggct
    25201 taaggtcctc accaagggtt acgagtggct taagtccaac aaggtcgagc tggttcgtct
    25261 gtgcgctaag ggaggactct aagatgattg acccgcgcac acctgagggc cgcatgactc
    25321 tccgttaccg tggctaccgg actgaagtcc tcctcagaga gcttggtcta gacccggaag
    25381 atgagacccg ccagcaccaa tcccgagacg agctgatagc gcaactggtc gctatgaaac
    25441 tgtctgccta attttaaaac cttttgagat gaccgatgcg gggagacccg taggcgctac
    25501 tgcgtctgcg ataccgatag tctcaactct caagaggaga attacctatg atgttcaaca
    25561 acaacaactg gaagctgtca gtcaccgaca tcaaccttta tgaaaacact gtgagtctgg
    25621 acggtcagtc ctatccgttg tcgctcgcta tcaagaccct gattccgggc tacctgtcag
    25681 gactgccgtc taccagccgt gagtctatgg agttactgga agctctggct gaagctggtg
    25741 tagctattgg caacttcttc agtaatgacc tgatgaccgc ctatggtcgc cgccagcaga
    25801 acaagcgagc tgaggctgag cgtatcgcta aagagcagcg cattcaggcc gagcgcatgc
    25861 gtgaagagaa catgactgat gcagagtggc agaaagagct acagcgccgc gaacaggtca
    25921 aagctgaacg ccggacctat ggtgagaacc tccgcagtgc tacccattcc gctggtcgct
    25981 cccgtgccgc tattgtcgct gaccttgagt caggcggtaa ctggctggac agcttataag
    26041 acacacagtc cctgacccga tgttagggac tcctcagttt tgaggactca cagtatttaa
    26101 actaatttag gagattgatt attatgacga ttaaagacgc tttccagttt ggcattgagc
    26161 cagtacgcat caccgatacc gacaacattc aggtcaatga aggtctgccg actaacgctg
    26221 acccgcaggt ctacgccctc cagttagcta agacggttaa ggctatgctg aacggtgtac
    26281 ttaaggacgc tcaagagaac atcccgttcc ctgtagaagt cctgccgacc cgtaacagct
    26341 taccgacccc aatcattgcc cacactctga ctgaccgctc tgtggtcgtt cctgtccgtg
    26401 gtggcaagcg acctgaagtc gtgaccgttc cgtctggaca agaaatcgtg gtcgaaccaa
    26461 tcgagcaggc tattctcatc tctgagcaga ccaagctctg ggacgccaag tcatccaccg
    26521 gattcactca gggcacactt cagcaggacg ctatgaacat ctgtgagaat gtggtccgta
    26581 ccatcaacgc tcgcatggtc gacgttctgg aatcctctaa gctgctgaag accgtagagc
    26641 ttcctgtcct gactggtagc ctgaccgcta aggcagacgc tatcatggac gctctgtatg
    26701 agaacacaga gtcttctttc ggttctgagg tgtctgacta cggtattatc gctcatgaga
    26761 gccaactgaa agcactgtcc cgtctggctg ctaagcaagg cttctccggt gaagacgcta
    26821 tcgtcgatat gctgggaaca gacatcgcct actacaacgg tgaagacaag ggtgtattca
    26881 tgctggctaa acgcttcacg gctctgagct tcggttgctt ccgtcacgat ggcgagaata
    26941 ttacggtcgt cctttcacgt gatggcgact ctcagtccca cgacttggaa atcctcggta
    27001 aagtgttcgt tgtcgctgaa gctgctacga ccatcaagat gggcactggc tcagctactg
    27061 ccgtcctgcc tgtagtcaaa cgcctgaagt tcaccaagac cgaagcgtaa gaccaatggg
    27121 taagagcatt agtaaaggct tcaggtccat cgccaagggc ctgacaggtg gtggtgggac
    27181 tcaaggtggg gcttcggtcc cgcctcctgt agctcctcct gtagctcctc cagatgacgc
    27241 tcagcaagct gtagtcgata cccaaggtca ggagaccgag ggaggtaaga ccatgagcag
    27301 aggtaagtct ggactgaata tcagcaggac ttcaggtggt ggcctgtcac tgtgattaag
    27361 acatctatac agcctgtgga gggtggacga tatcaagcaa caatctggat gacacgcaga
    27421 gtctataagc ggctatgctg gtcactggct gaagctgaac agtgggtgag acgtaaagtg
    27481 cctaccgttc aatcgttcca atcatcatga taactatggt ctggaccaat ggtctggacc
    27541 tttgttttgt actagttatc tagtgcagta gtacaataga cgaaggaggc tttatgtcta
    27601 tgacttccgc agacctaagg tccctcctga ctctggtcta taaactcgtg ttcctctctg
    27661 tgggtctcta tatggtcctc tccggtcgac tcggtgtgaa tgtattcgac actctgtcta
    27721 aggctgtggg tgggcttctg ggggcttagg tttggactca cagtgggacc gatggtttct
    27781 gacagaaaaa tgtgaatgac catctaatac attaagccag cagacttccc ccatagcccg
    27841 cctcaagtcc agaccgaggc caccgggggg ctgaccagcc gtctagacat cccgtctaat
    27901 gacacaaggc tgacacaaag cctgacacaa agtccttgac tcaatgcagg acctcaggcc
    27961 tactaagggt ccgcttcaag tccttgaagt tccagatgaa cttaatgggc acatccagtt
    28021 tatctttgcc gcggacgccg tcgcggattg ccgtcatttg cggaccatga tcgatagcat
    28081 ctgtccagct gaagcaggag attgacattt tgaccggatt atccagcacc ggcaggcgtt
    28141 ctatggtaat ggtataggtc gattcacgcg cgccactatt tccgccgctg atgccgacat
    28201 tgcccgtcaa aataggtaac atagcaatag cgcgtgcagt cagttcgccg tttgcctggc
    28261 gttgcggccc ccagccctgg cagatataag cgggttttgc tgtgccaatt tcacgcgcca
    28321 gtttgatgat acggtctacc gggataccgg taatttgcga agcccactgc ggcgttttcg
    28381 ctgtgttatc atcaccttca ccaagaatat aggctttata gtgactattt ttgggtgcat
    28441 ctgcgggtaa ggttttttcg tcatagccga cgcagtattt atcgagaaaa ggttgatcaa
    28501 cgagattttc gttaatcaat acccaggcaa tacccgcaac cagcgcggca tcggtgcccg
    28561 ggcgaatagg gagccattca tcttcacgac cagcagccgt atcggtatat cgcggatcga
    28621 tagcaatcat tttggcgttc gatttctcgc gcgctttttc aagaagataa gtgatgccac
    28681 cgccgctcat gcgggtttct gccgggttgt taccaaacat cacgaccagc ttgctgtttt
    28741 caatatccgt ggtgctgttg ccatcattac tgccgtaggt gtagggcatg gcacaggaaa
    28801 tttgcgcggt gctgtaggag ccatactgat tgagtgaacc gccgtagcag ttcatcaggc
    28861 gtttgaccgc cgaggctgat ggcgaagagc gggtcatatt gccgccaacg atccccgaag
    28921 agtactgaat atatacagcc tcattgccat attgttcgac ggttttcttc aggctactgg
    28981 cgatagtatc cagggcttca tcccagctaa tccgttcgaa tttgccttcg ccgcgtttgc
    29041 ccacgcgttt cattgggtaa ttcaagcgat cgggatgatt aatacgccga cggatggatc
    29101 gaccacgcaa acaggcgcgt acttgatgat tgccatactc gtcgctgccg gtgttgtcag
    29161 tttccaccca ggtgacttca ttatctttaa catgtagacg aagtgcacag cggctaccac
    29221 agttgacgga acaggcaccc cagatcactt tttcgctggc ctgttgtacc gctgccgctg
    29281 cactgcgcag ggtgaacggt aaagaaaaac cgcctgcagc cagcgccaga gaacctatcg
    29341 cggttgattt aacgagtgtt ctgcggctga tgcccaccat tcggtcattt ttggacataa
    29401 ctcactccct gttctttatc gttatataaa tgtttatata ttgaatattt agcgcgctaa
    29461 caatagaggg agtctaccca ttttgggtta agaattatta atccatatca atagaagggt
    29521 atgagtaata aggtgggatt atgttgtatg ttcaaatcgc cggatgtgtc gtatccggcg
    29581 ttcagtcgat aatgtattac tgcggttcgg caggcgcgcc atcctgggta gactgcgcgg
    29641 gagcagagac gttaccgctg gtggtgcggg tatagagaat tttatgcgta tcattagcgc
    29701 aatggccgac gacctgggaa tcaggctgat caacctggtc attgggtaca atacttaacg
    29761 tgaagctgct ttcgggtacg ccattattga taatgcgctg tgatatatcg ctctgtatgc
    29821 gctcacagga tcccggcgcg gcgagtaccg cgggtgaggc gagggcgagc agaagcgcgg
    29881 cacagcaggt tgagagtttc atcataagct ccttacgcga agataacttc tttaagcata
    29941 gcatttaacg tgtaaagtac tgtatttgct actatgattg agaatcatct ctactctctg
    30001 gtgactgttg tgaaatacaa attactacca tgcttactcg cgatattcct cacaggatgt
    30061 gaccgcacag aggtaacact ttcatttacc cctgagatgg ccagtttctc taatgaattc
    30121 gattttgatc cgctgcgtgg tccggtaaaa gatttcactc agacattaat ggatgagcaa
    30181 ggtgaagtga cgaaacgtgt ttctgggact ttgtcggaag aaggctgttt tgattcactc
    30241 gaattactgg atctggaaaa taataccgtg gtcgctctgg tactggacgc caattattac
    30301 cgtgatgccg agacgctgga gaagagagta cgtttacagg gaaaatgcca gctagcagaa
    30361 ttaccttctg ccggggtgag ttgggaaacc gatgataatg gcttcgtgat taaagccagc
    30421 agcaaacaaa tgcagatgga atatcgctat gatgatcagg gttatccact gggtaaaacc
    30481 acgaaaagta acgacaaaac attatctgtc agcgccacgc catcaacgga cccgatcaaa
    30541 aaattagatt acacagcggt tactttactg aataatcaac gagttggtaa tgtaaaacag
    30601 agctgtgaat atgacagtca cgccaatccg gtggactgtc agctgatcat tgttgatgaa
    30661 ggagtaaaac ccgccgtcga aagggtttac accatcaaaa atacgatcga ttattattaa
    30721 tgctattgtg cggtcggctt caggagagtc tgacccggtg ttttgtgctc tgccagatac
    30781 tgatgctgga atatacacat gcgaatggca ttacgatatt gaccattaat aaagaactcg
    30841 tgcatcaatt caccttcaac cgtaaagcca agcttgcggt aaatgtgaat cgctttttca
    30901 ttctctttat cgacgatcag atacagctta tagagattga gaacggtgaa gccatagtcc
    30961 attgctaatt tggcggcacg ggttgccaga cctttcccct gatactccgg ggagataatt
    31021 atctgaaatt ctgcccggcg atgtacatgg ttaatttcca ccagctccac cagaccggct
    31081 ttttcgccgt cacattccac cacaaagcgt cgttcgctct gatcgtgaat atgcttatca
    31141 tacagatcag agagttcaac aaaggcttcg tagggttcct caaaccagta acgcatcaca
    31201 ctggcgttat tgtcgagttg atgtacatag cgtaaatctt cacgctccag cgggcgtagc
    31261 ttaacactgt gggcgcttgg cataacgtgt ccttacattc cttaaatcaa taacaggtta
    31321 gggggtaata acgcggccag ttcgacggtc caggcagcgc aaagtatttg gctcccagta
    31381 ggcattgatg ttggcgcttt gctcacattt atcgcggtta tcaaaagcgg cgtcggcttt
    31441 atcccactct ttttcagtgc gtttattcac tttctgacgc agattgcgcg tgtcattcca
    31501 ttgctctttt tccatagcgg cgtgctggcg gctttgtgca ctgtcgccag actcaatcac
    31561 cagtttgtta gtttcggcat gaacagttgt gctcagtgcc agtgcgcaag gcagcagaaa
    31621 agcgagcagg ccgattcgtt tgctgagagt gattttcata attcattccc tgtatgaatg
    31681 attaaaggag attctacacc atccactgcg aacgcaaaac gtaccaggag ggtgtttata
    31741 ttgatgatat tatgtcgccc tataactata catgatgtca ataagtgaca aagatgatta
    31801 aagcaacgtt actatttttt gctactgcgc tgtgtgaaat tattggatgc tttctgccct
    31861 ggttgtggtt aaaacgaaac gccagtatct ggctgttgct tccggcgggg atttcactgg
    31921 cgctgtttgt ctggttgtta acgttgcatc cagcggcgag tgggcgtgtt tacgcggctt
    31981 atggtggcgt ttatgtctgc acggcgttga tgtggctgcg cgttgtggat ggcgtgaaac
    32041 tgactcttta tgactggacg ggtgcgttga ttgcgctttg cggcatgttg atcattgttg
    32101 cgggctgggg gcgcacgtag gaacataaat ccattttatc aataagataa gaggaagtgt
    32161 cagctgacaa aaggtattct atttcatctt ttgtcaacca ttcacagcgc taatatacgc
    32221 ctttttttgt gatcactccg gcttttttcg atctttatac ttgtatggta gtagctcagt
    32281 tgcgtagatt tcatgcatca cgacaagcga tgcaaggaat cgaacatgaa gatcgtaaag
    32341 gctgaagttt ttgttacctg tccggggcgt aatttcgtca cattaaaaat caccactgag
    32401 gacggtatta cgggccttgg ggatgccacc ctcaatggac gtgagctttc cgtggcctct
    32461 tatttgcagg atcacctttg tccgcagctt attggtcgcg atgcgcaccg tatcgaagat
    32521 atctggcagt ttttctataa aggtgcttac tggcgtcgcg gtccggttac gatgtcggcc
    32581 atttcagcgg ttgatatggc gctgtgggat attaaagcca aagctgccaa catgccgctt
    32641 taccagttac tcggcggcgc gtctcgtgaa ggggtgatgg tttattgcca taccaccggt
    32701 cacagtattg atgaagctct ggatgattat gcccgtcatc aggagctggg attcaaagcc
    32761 atccgcgtgc agtgcggaat ccctggtatg aaaaccacct acggcatgtc gaaaggtaaa
    32821 ggtctggctt atgaacccgc aaccaaagga cagtggccgg aagagcagtt gtggtcgacg
    32881 gagaaatacc tcgatttcat gccgaaattg tttgacgcgg tacgtaacaa gtttggtttt
    32941 aatgaacatt tgctgcatga catgcaccat cgcttaacgc ctattgaagc ggcgcgcttt
    33001 ggtaaaagca ttgaagatta tcgcatgttc tggatggaag acccgacgct tgcagaaaac
    33061 caggaatgct tccgtctcat tcgccaacat accgtcacac ccatcgcagt gggtgaagtc
    33121 ttcaacagca tctgggactg caaacaactg attgaagagc aactcatcga ttatatccgc
    33181 accacgctga cccatgcagg cggaattacc ggtatgcgcc ggattgccga ttttgcttcg
    33241 ctgtatcagg tacgtactgg ctcacacggt ccttccgatt tgtcaccagt ctgcatggct
    33301 gcagcgctgc actttgatct gtgggtcccc aatttcggtg tccaggaata catgggttat
    33361 tccgaacaaa tgctcgaagt cttcccgcac aactggactt tcgataacgg ctatatgcat
    33421 ccgggagaca aaccgggtct tggcatcgaa ttcgatgaaa agctggcggc gaaatatccc
    33481 tatgaacctg cttatctacc agtcgcacgt ctggaagatg gcacgctgtg gaactggtaa
    33541 ggagtaagat aatgaaaagc atattaattg aaaaaccgaa tcaactggcg attgtcgaac
    33601 gtgaaatacc caccccgtca gcgggtgaag tacgagtaaa agtgaaactt gccggaattt
    33661 gtggttcaga tagccatatt tatcgtgggc ataatccttt tgcgaaatat ccgcgcgtca
    33721 ttggacatga attctttggc gtcattgatg cggtgggtga cggcgtggaa agcgccagag
    33781 tcggtgaacg tgttgctgtc gatccggtgg tcagctgtgg gcattgctat ccgtgctcta
    33841 taggtaaacc gaacgtttgt acgacacttg ctgtattagg tgtgcacgct gacggtggtt
    33901 tcagtgaata tgccgtggtt ccggcaaaaa atgcgtggaa aattcctgaa gcagtggccg
    33961 atcaatatgc ggtgatgatc gaacctttta ccattgcggc taacgtaacc ggacatggtc
    34021 aaccgactga aaatgatacc gttctggttt atggtgccgg tccaatcggc ctgacgatcg
    34081 ttcaggtatt aaaaggcgtc tataacgtta aaaatgtgat tgttgccgat cgcattgatg
    34141 aacgactgga aaaagcgaaa gagagcgggg cagacagggc gattaataac agccagacac
    34201 cgcttggcga gattttcgct gaaaaaggca tcaagccgac attaattatc gatgcggctt
    34261 gtcatccttc tatcctgaaa gaggccgtta cgctggcttc tccagcggca cgtattgtat
    34321 tgatgggctt ctccagtgaa ccgtctgaag tgattcagca aggaattacc ggaaaagaac
    34381 tctctatttt ctcttcacgc ttaaatgcaa ataaatttcc ggttgttatc gactggttaa
    34441 gtaaagggtt aattaaacca gaaaaattaa ttacccatac gtttgatttc cagcatgttg
    34501 ctgatgccat tagtttattt gaacaggatc aaaaacattg ctgcaaagtc ttactcactt
    34561 tttctgaata ataccaataa cggcgagtaa gtagtacgca tcttacctct tttttagaga
    34621 taaccattat gacaatagaa aaacatgaaa gaagcactaa ggatttggtg aaagcagcag
    34681 tatcgggatg gctgggcact gcgcttgaat ttatggattt caagagtcat gcgtgttaac
    34741 tatttgataa atattaaatt aatttttcat tgattcgtta tggggcatgg ttggggcaaa
    34801 ctcgcttaac tgtgtattta acaaagctac ctgtgcatta ttgttttcag acatccattt
    34861 tccgtatacc tgaaatacca tttgcgcatc tgcatggccc atctggtttg ctataaatgc
    34921 cgggttagca ccagctgtca gcgaccagca ggcataagta tgtctcgact gatatgattt
    34981 tcgatggcgg agtccggcac gttttatcgc tgcgtcccac atctgcctta ttgagtcaac
    35041 ggtaaaatgg tcaccataat tttttactct cgctgacact tcaggttgaa aaacaaaggt
    35101 gcatttttgt ttttctgttc tgccatactc tctgaggtga acatcaatga tatgctcttt
    35161 gctcagtctc gttaatgtca tctgactccg gagagcgtcg attgctggct taataagatg
    35221 aatggcccga ttggttcccg cctgtgtttt tggtaccgtg aaacggtctt ttgctaaatt
    35281 tctcctgatc atcattgttc catttttcag atctatgtcc tcccatccaa gtgcacacag
    35341 ctcaccaggg cgaacgccag tataaacaga aacacaccat aaattttttg cttgctgatt
    35401 tctgcacgca tcgataagac ggataaattc ctcccgcgaa agaggatccg gaatggttct
    35461 tgattccttt aatggcgaga tccccttaaa cggattatct gccaggtaac cgttatcaac
    35521 accaaactgg aacacggcgt taagatttgt catgtaatta tttacagtta cagccgatct
    35581 ccctggttgt gtaacaatat agttactttt ggggatctgg tatccagtca gtagctcttt
    35641 acgaacctcc agtaattttt ctttattaat cgatgaggca agattttttt caccgattat
    35701 gctcaggata tttttgatga cggcacggta tgtgttgagt gatgttttgg cgacttcagt
    35761 ttctttcagt gccagaaatt tttcagccag ttcttttatg gttaaatctt gtcgggcctc
    35821 accaaatttt tccagattgc gtgaggaggg aaactgtttt gcatagtcga aaacaccagt
    35881 ttttattgcg taacaaacag aggagcgtag ttcacctgca acgcgcctgt tttttgctgt
    35941 gtcaggaacc cccagatttt ccctgactct tacgccttta taaacaaacc agatacgtaa
    36001 tttccctcca tggttttcca cgcctgtcgg atatttcatt tcaacttctc tcattagtta
    36061 gtgtggcttt tagtcaagta agatgacatc ttggtctcgc tgatgcttgg cgctcaatcc
    36121 agcgatcaat ttcttccagg ttgtaaaagc atggactgtt atcccatggc ataccgtcat
    36181 gagcgacatg cttatattcc cttccttcca taaacgattt ttcccgagcc ttttttaacg
    36241 tacctttttt tattcctttc agcgcaatta actgctcttc ggatacccat ttgccgggag
    36301 agacaatcat gattacttcg ctcatcgatt tctttatctc ttacatcaga cgagcgccgg
    36361 ttgcagaata ccagtcacaa ccggcgacag ttgaacatta agaatcagcc tgactcggga
    36421 tcagtttttg ccagataact gaaacgtatt ttgcctggta acgggcgtca tcaagtgcat
    36481 tatggcgctc accttcgaat ggaatagccg ttctggcatc gaagtctatg gctttcccca
    36541 gctcaacgat tgtgcgtaca tcgcgatcgt tgtagtaacg ccacgggcag gggatcccct
    36601 gccgttcata tgaacggcgc aaaatcgtgt tgtcgaagtt ggctccattt ccccaaacct
    36661 gaacaaaaaa ttcaccggag ttttcgtcga taaattcccg caattgtaac agtgcatcat
    36721 ctaacgggat ttcatcggtc ataatggcag attgcgcttc gcgtgattgc ttaagccacc
    36781 atttaatggt gtcccgatca atgactccgc cagcagtttc cagatcgata gtcttactaa
    36841 attccggtcc catatctccg gtttgcggat cgaaaaatat tgcacctatt gagatgatcg
    36901 gggcatcagg attttttccc atggtttcaa ggtcgatcat tagatggtca cacgtcctgc
    36961 tggtggatgt gatttcgtga tgaccgttca ccttaattgg gtgatctgcc gtctcgccag
    37021 tttcattatc gctattgtga tgctgattgc cgccagtgtt ctccttgtgt ggatgttcag
    37081 cgccttccat ttcctccgga tcatcttcct gaacttcaac ctgatactct tcatcgaatg
    37141 tttcttggta tgttgcgtcg cccatcaccg cgccacaatc agggcagttg ccgccgccgg
    37201 tctgaccgca ggcggtgcag accttttcca cttcctgttg cgccactggt tcaggctgtt
    37261 tcgtttctgg ctcgttttgt aacgcatttg gactgttttg ttccgctttt tggtagttcc
    37321 gttccgattc atgctggttc tggttcacag aatcgcgggt ctggatcccc ttaacccatt
    37381 tcggatcatt cgggtcacta atcccttcaa caaattcacc acgtgatgca gcaagcaact
    37441 tatcggcgtc aggctggctg atattggctg cctgcataat tttgtttact tcgtcagcgg
    37501 taacttttat cggctctggt tgttctgaat cttcagcggt atctacattt tgcggtaagc
    37561 ccgtgtatgt gccatttttt cgggcaaaat attcttcttt tgtgatttca gtggcgccag
    37621 cagccagtgc cttatccaga ccagaaagtt tgtttgcgcg accgtatttt tctccgtcct
    37681 tatctgcgaa gaggaaatag aacggcccct cacgctctac agatggttca gcttccggcg
    37741 cggtttcatt ttttgggata tcagatacct cagtttccac tgcatcagtt tgtgtttctg
    37801 atgactggag aacatcaaca gtgcccaggt ctgtttcttc attctcaaac acgccctttg
    37861 tcgtcaggta ttcgcagata tatttgttca gtgctacggg atctttgtga atgtcgatcg
    37921 gacgctcacg gacaaggcca aaaatagttt ggcggtcgta gcgaagggca tcaggctgtt
    37981 tgcgcattga tgccgagata cgcttccagt cttcgcggtc gttgtcgata acttcttttt
    38041 ttgcccagcg atggatgctg ccgtcaatgt ttccggcatc cacatcacca ggccagagag
    38101 cgtaggccag ttcgtcatcc agtgttttcc atgtctgctt gtattcgcga tgaatggcag
    38161 caatgaccgg gctgattttt cctgttgaat tttcagtgta ctgttgattg gctctggcgc
    38221 gggcgagatc aacaacagac gtgtattttc cggtttcctt gcgttcacct tcgcgacgtt
    38281 ttttccagat gcgcatctct gcctgaattt cgggccattt agcaccagga atacatttat
    38341 gcttaaccca cccaatggcg tgcaacttaa gctccggata catggcgtta acttctggca
    38401 ttttcatcaa cgcttcaacg atatgtccgt cgaatgttgc catgtcttcc tgcaacaatt
    38461 cctgtgcgct aataaccata tcaacggtga tgttttcaca tgtgtcgaac ttaaccatga
    38521 cagcgttctg tacttcaggg gccagcttgt caaaagtgac gttcatcgga tcggattcag
    38581 tctcaaccgg gacaaaagaa gcagactcct catcccagcg gttttcctgc atatattcag
    38641 catcccatga atcgagggca gggcggggta tgccaggttt atcctcgcag acaataaatt
    38701 tataagcgca gtcctgagca gccggataat gttccaggaa ttgccagtga aattttgctc
    38761 gagcacggcg ttcgtcgcca gcttcaatgg ctgtggctac agccacagcg ccttcttccc
    38821 ttgttgccag ttcgtcagga atagcggcgc aaataaagac tttactcatt ttgttttaac
    38881 ctcattacag atttcagggt gaacgaatcc ctgccattgc tggcattttt aatccgttgg
    38941 tatggcgtta atatggctgg cgggttatcc agccggtatt tcgttattca ggttcagcga
    39001 tacttttttt aacgggaggc attcaccggg gattttttgt tcgtccctta cctgaatgca
    39061 ggatgactta ctgtcataaa ttccggtaat cacattttgt ggctcacccg ttataagaaa
    39121 aacggtcatc accagtgcaa atgctgaagt cactgctgtt ctccgataat accaagttca
    39181 agaagggcaa ttctggaaag tatggaatta tcattgagaa gataaggttc atattttctc
    39241 atcttaatgg catcttccgt aaactcccgg ttactgagca gaacaccaat atcaaaacaa
    39301 ccttcagacg tattaacgtt tggcagtgac gtttccatta tctcgtcctc aacaatgaat
    39361 ttttagtaat tgctccacag tcatattttt aatcgcgctc cggtttacaa gagtccagcc
    39421 ttgtttctcc agataaaact ggaaggtatc cagggtgcag accattgcgc cgtccggaac
    39481 ggtttcagtg aatttgatat tgccgtgttc gtcgagacgg ataaccaggg tacgtccgtc
    39541 cccgtgaatc attttgtcgg gagactgggc gttattctgg cgcagttctt tctccatgcg
    39601 atcgaactca gcaatgtagg cttctttaaa tgcagcggct tttttgccgg tgaagcccat
    39661 caccaggaaa acgaagccgt ttttggtgat ttggtacatt gggagtttgc gaccggttga
    39721 gtcggtatat tcgctcgaca caaaattgtg ctcagtgaat tttgctgaac agtccagatt
    39781 gcgaatttta tccaacactc gttcgtggcg tttaccaaag aactcagcga tcgcaataga
    39841 cgtagtgaca gcgcgtccat tttcgatggt tacgtcaggg tgagaaaggg cagggatagt
    39901 agccatgatg gcagcctcga tagtcagttt aaataactca ccaccaaggc tttccacgac
    39961 cataagggtg gtgagacgta caggggtgga aataccggtg actaccgaaa ccggccagcc
    40021 tttcggctgc cctgcacgcc ccaccataat ctgaatgtgg ctgtgcatta cgcataaaaa
    40081 aaccgcctga gcgcggttat gcgcagtagt catcatcggg tttccacgcc cggcacccgc
    40141 tttatgaggt gcaggtgcac tataattcca cccgttctgg ttttcaatag ctacattcaa
    40201 cattttctct tacccttcat taccgaagtg aactttgttg atgcagtgcc tggtgcctcc
    40261 aggtgacatt aaccagttaa caattaacgc cggattgtta gttgatgtct gttacgcaag
    40321 taaaagaccg cttgttttaa ctgttccgcg tgcgcatagc cgcattcacc gcatcacaaa
    40381 attcacttta aaaagggtgg cagagcagtc acggagtaga actgatgcca ccaaagacta
    40441 cacatgggta ttgtggcggg gctgtcactt aagcgtatgg tcaacctgac aacccggtgc
    40501 attttctgga gcaatggagg aaaccccagc catacttacc gccgcgccat ttcgcggatt
    40561 gccacaaccg gaagcgcacg atcgaattaa atttaacgat gccgtacaga gagacgaact
    40621 tcgccgtgcg ctttcgtgtt atgccctgac ttttcaggga aatatccttt cagcaaactg
    40681 tcagtgccgg attcttatcc gtgtccggcg cacgcgctct acctcacccg tggagaacta
    40741 cttaattacc aaccttagct tcgttggtta gctattaacg cgggtatgta atcattctgg
    40801 caatgcttaa tgccgctgct ttttccagcc tggtgatatc ctgctccaga gcggacagat
    40861 tttcagcctg cttagccctg gcttcattgg cccatttcag atcctgcgct gcattaattt
    40921 tctggcgcat ccactcataa agttcatcat cggtatagtc tggtgcgatg atgacgggtt
    40981 ctcgtttctg catactgatt cctcgcggtg ctgtttcgct tatcagccgt tagattttgc
    41041 cgagctggaa agcgcctgtt taaattcgtt gaagctgaga gcttcttcgc cgtcggcaag
    41101 accttcgaag tattcttcat aagccttttc catgattgtg tcgaaatcca tatcactcac
    41161 ctgagtttct ttccagccag cgacgggcac cattttcggt tttaaacgtt ttgcttttgg
    41221 tatacgtcat cgcggtgaac gtgccgtcct ggttggggaa cacgccacat accagagatt
    41281 cgttgttgcc aagatcgata gtatccatgc tgacctcatt tccccttaac gccggggtag
    41341 cggaacaaaa acctgc
//
LOCUS       DAZGVQ010000025        40092 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000025 DAZGVQ010000000
VERSION     DAZGVQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.025"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..277)
                     /gene="chbB"
                     /locus_tag="ACHLAG_001293"
     CDS             complement(<1..277)
                     /gene="chbB"
                     /locus_tag="ACHLAG_001293"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFP0654554.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGV"
     gene            complement(576..914)
                     /gene="osmE"
                     /locus_tag="ACHLAG_001294"
     CDS             complement(576..914)
                     /gene="osmE"
                     /locus_tag="ACHLAG_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFP0654555.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1116..1943
                     /gene="nadE"
                     /locus_tag="ACHLAG_001295"
     CDS             1116..1943
                     /gene="nadE"
                     /locus_tag="ACHLAG_001295"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFP0654556.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2173..3060
                     /gene="cho"
                     /locus_tag="ACHLAG_001296"
     CDS             2173..3060
                     /gene="cho"
                     /locus_tag="ACHLAG_001296"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFP0654557.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3020..3595)
                     /gene="ves"
                     /locus_tag="ACHLAG_001297"
     CDS             complement(3020..3595)
                     /gene="ves"
                     /locus_tag="ACHLAG_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFP0654558.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3798..4283)
                     /gene="spy"
                     /locus_tag="ACHLAG_001298"
     CDS             complement(3798..4283)
                     /gene="spy"
                     /locus_tag="ACHLAG_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFP0654559.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4613..5581)
                     /gene="astE"
                     /locus_tag="ACHLAG_001299"
     CDS             complement(4613..5581)
                     /gene="astE"
                     /locus_tag="ACHLAG_001299"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFP0654560.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDHRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5574..6917)
                     /gene="astB"
                     /locus_tag="ACHLAG_001300"
     CDS             complement(5574..6917)
                     /gene="astB"
                     /locus_tag="ACHLAG_001300"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFP0654561.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6914..8392)
                     /gene="astD"
                     /locus_tag="ACHLAG_001301"
     CDS             complement(6914..8392)
                     /gene="astD"
                     /locus_tag="ACHLAG_001301"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFP0654562.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHARTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8389..9423)
                     /gene="astA"
                     /locus_tag="ACHLAG_001302"
     CDS             complement(8389..9423)
                     /gene="astA"
                     /locus_tag="ACHLAG_001302"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFP0654563.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9420..10640)
                     /gene="astC"
                     /locus_tag="ACHLAG_001303"
     CDS             complement(9420..10640)
                     /gene="astC"
                     /locus_tag="ACHLAG_001303"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFP0654564.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            11086..11892
                     /gene="xthA"
                     /locus_tag="ACHLAG_001304"
     CDS             11086..11892
                     /gene="xthA"
                     /locus_tag="ACHLAG_001304"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFP0654565.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12059..12769
                     /locus_tag="ACHLAG_001305"
     CDS             12059..12769
                     /locus_tag="ACHLAG_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP0654566.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            12774..13451
                     /gene="ydjY"
                     /locus_tag="ACHLAG_001306"
     CDS             12774..13451
                     /gene="ydjY"
                     /locus_tag="ACHLAG_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFP0654567.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            13466..14173
                     /locus_tag="ACHLAG_001307"
     CDS             13466..14173
                     /locus_tag="ACHLAG_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP0654568.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            14173..14721
                     /locus_tag="ACHLAG_001308"
     CDS             14173..14721
                     /locus_tag="ACHLAG_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP0654569.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14731..15897
                     /locus_tag="ACHLAG_001309"
     CDS             14731..15897
                     /locus_tag="ACHLAG_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP0654570.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            15870..17405
                     /locus_tag="ACHLAG_001310"
     CDS             15870..17405
                     /locus_tag="ACHLAG_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP0654571.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFAEGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            17405..18058
                     /locus_tag="ACHLAG_001311"
     CDS             17405..18058
                     /locus_tag="ACHLAG_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP0654572.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            18125..19432
                     /gene="ynjE"
                     /locus_tag="ACHLAG_001312"
     CDS             18125..19432
                     /gene="ynjE"
                     /locus_tag="ACHLAG_001312"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFP0654573.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTPDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(19441..20061)
                     /locus_tag="ACHLAG_001313"
     CDS             complement(19441..20061)
                     /locus_tag="ACHLAG_001313"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP0654574.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            20148..20555
                     /gene="nudG"
                     /locus_tag="ACHLAG_001314"
     CDS             20148..20555
                     /gene="nudG"
                     /locus_tag="ACHLAG_001314"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFP0654575.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(20521..20793)
                     /locus_tag="ACHLAG_001315"
     CDS             complement(20521..20793)
                     /locus_tag="ACHLAG_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFP0654576.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            21029..22372
                     /gene="gdhA"
                     /locus_tag="ACHLAG_001316"
     CDS             21029..22372
                     /gene="gdhA"
                     /locus_tag="ACHLAG_001316"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFP0654577.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(22489..23531)
                     /locus_tag="ACHLAG_001317"
                     /pseudo
     CDS             complement(22489..23531)
                     /locus_tag="ACHLAG_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
     gene            complement(23659..25620)
                     /gene="topB"
                     /locus_tag="ACHLAG_001318"
     CDS             complement(23659..25620)
                     /gene="topB"
                     /locus_tag="ACHLAG_001318"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP0654578.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQSVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRALL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(25625..26668)
                     /gene="selD"
                     /locus_tag="ACHLAG_001319"
     CDS             complement(25625..26668)
                     /gene="selD"
                     /locus_tag="ACHLAG_001319"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFP0654579.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(26785..27336)
                     /locus_tag="ACHLAG_001320"
     CDS             complement(26785..27336)
                     /locus_tag="ACHLAG_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFP0654580.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            27497..29353
                     /gene="sppA"
                     /locus_tag="ACHLAG_001321"
     CDS             27497..29353
                     /gene="sppA"
                     /locus_tag="ACHLAG_001321"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP0654581.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            29520..30536
                     /gene="ansA"
                     /locus_tag="ACHLAG_001322"
     CDS             29520..30536
                     /gene="ansA"
                     /locus_tag="ACHLAG_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFP0654582.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            30547..31188
                     /gene="pncA"
                     /locus_tag="ACHLAG_001323"
     CDS             30547..31188
                     /gene="pncA"
                     /locus_tag="ACHLAG_001323"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFP0654583.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHCVEPYMPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(31349..31621)
                     /locus_tag="ACHLAG_001324"
     CDS             complement(31349..31621)
                     /locus_tag="ACHLAG_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFP0654584.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(31663..32076)
                     /gene="msrB"
                     /locus_tag="ACHLAG_001325"
     CDS             complement(31663..32076)
                     /gene="msrB"
                     /locus_tag="ACHLAG_001325"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFP0654585.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            32418..33413
                     /gene="gapA"
                     /locus_tag="ACHLAG_001326"
     CDS             32418..33413
                     /gene="gapA"
                     /locus_tag="ACHLAG_001326"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP0654586.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            33497..34381
                     /locus_tag="ACHLAG_001327"
     CDS             33497..34381
                     /locus_tag="ACHLAG_001327"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFP0654587.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVTLTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(34432..35286)
                     /gene="yeaE"
                     /locus_tag="ACHLAG_001328"
     CDS             complement(34432..35286)
                     /gene="yeaE"
                     /locus_tag="ACHLAG_001328"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFP0654588.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTDLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELIMLDKAYPAPK
                     GKTALDMV"
     gene            complement(35376..36122)
                     /gene="mipA"
                     /locus_tag="ACHLAG_001329"
     CDS             complement(35376..36122)
                     /gene="mipA"
                     /locus_tag="ACHLAG_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFP0654589.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            36558..38492
                     /gene="yeaG"
                     /locus_tag="ACHLAG_001330"
     CDS             36558..38492
                     /gene="yeaG"
                     /locus_tag="ACHLAG_001330"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFP0654590.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            38605..39888
                     /locus_tag="ACHLAG_001331"
     CDS             38605..39888
                     /locus_tag="ACHLAG_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFP0654591.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         9714 a        10509 c        10052 g         9817 t
ORIGIN      
        1 gcacgcctaa accatcgact ttgccataaa gcagcgagtc aattacttca accggtttgt
       61 tgggtaacaa acgctggatt tcgggcaaca tataagcaat ctgcggccct aataacacga
      121 catcggcatt ctgacctttt tcaccagcca gtgtttccgg aaatgcttca ataatgaccg
      181 gaacttcata tttttctgcc tgtgcgcgca tttttgatac cagtaaagag gtagacatgc
      241 ccgcagaaca aaacagataa atgtgtttct tttccataaa actgccctca tcgacgatta
      301 tctgtcagcc agacactccg caagccttaa cctgcttcca tgctctgggt aacttgcgaa
      361 accaacatga ctttttttgt ggctgaaagg agtatacggt atagacctga gcaatagtat
      421 ttctttgacc ttataaattg tttctccttg acctgtggtt atgaccctct tcacatttcg
      481 ggcaaataat tcgcgaagat aattaagaac tcaggcccaa aaaaaccggc gcaatggccg
      541 gtttccgttg ttactcaagc tttcagacga attgattact tcgcagcctg tggatcagtg
      601 tcgtattcag cacaggtctg ataaccggag ttgatgacat gtccggtatc atctaacgcg
      661 acaaagtagg tttctgcttt accatcacgt tgacccagga tgtaggtctg gcaagtaccg
      721 cgagcatgga tcatgctcac ttcagacgaa ggtttacccg caatttgtgc aacctgcgcc
      781 cggctcatgc cttttttgac gtctttcacc acaggctgta caaactggtc tttagtacga
      841 tcataagccg tacaacccgc cagcatggtt aataccgccg ctgcactcag aattcctgcc
      901 atattcttgt tcatattccg tcctcttgtt tatcagcgtg ttagataagc ctggaataca
      961 ttgggcgctt tttcaagccc gtgaacgaaa cagctccgct ttcagaggat tcctgtatga
     1021 cgttttaacc accattcagc ccgctgtcgc ttgtcgtttc agtagcaacg ggttagcttt
     1081 aaggaagttt tgtcttttct gtctggaggg gttcaatgac attgcaacaa caaataataa
     1141 aggcgctggg cgcaaaaccg cagattaatg ctgaagagga aattcgtcgt agtgtcgatt
     1201 ttctgaaaag ctacctgcaa acttatccgt tcattaaatc actggtgctc gggatcagcg
     1261 gcggtcagga ctccacgctt gccggaaagc tgtgccagat ggcgattaat gagctgcgcc
     1321 aggaaactgg caacgaatca ctgcaattta ttgccgtacg cctgccctat ggtgttcagg
     1381 ccgacgaaca agattgccag gatgccattg cctttattca accggatcgc gtattaaccg
     1441 ttaatatcaa gggcgcggta ttggctagcg agcaggcatt gcgggaagca ggcattgaac
     1501 tgagcgattt tgtccgtggc aatgaaaaag cgcgtgagcg gatgaaagca caatatagca
     1561 ttgcgggtat gaccagcggt gtcgtggtgg gcaccgatca tgcagcagaa gccattaccg
     1621 gattcttcac taaatatggt gacggcggta cggacattaa tccgctgtat cgtctcaaca
     1681 aacgtcaggg taaacagtta ctggcggcat taggttgccc ggaacacctt tataagaaag
     1741 cgccaacggc cgatctggaa gatgatcgcc cttctctgcc agatgaagtg gcactcggcg
     1801 tgacctatga caatatcgac gactatctgg aagggaaaaa cgtacctgaa caggtcgcca
     1861 gaacaataga gaactggtat ctgaaaaccg aacataaacg ccgtccgcca attaccgttt
     1921 tcgatgattt ctggaaaaag taataatttg cacctattgg attgtgcgaa aaagagtaat
     1981 ttgttcacgc cggatgcggc gtgaacgcct tattcgacct acaaaactat gcaaattcaa
     2041 tacattgcag gagtcgaata ggcctgacag gcgtagcacg tcagacggtg taacctttgt
     2101 catcaacccg cctctttttt aatcgcctcc cgcctgttac actggataga taaccagcat
     2161 tcggagtcaa cagtggtacg gcgtttaact tctccgcggc tcgaatttga agctgcggca
     2221 atttatgaat atcccgaaca tttacgttca ttccttaatg acttacccac ccgaccaggg
     2281 gtgtatctgt ttcatggcga aagtgacacc atgccgctct atatcggcaa aagcgttaat
     2341 atccgcagtc gggtgctttc tcatttacgc accccggatg aagccgccat gctacggcaa
     2401 tcccggcgaa ttagctggat ctgcaccgca ggcgaaatcg gcgctctgct ccttgaagcg
     2461 cgattaatca aagaacaaca gccgctgttt aataaacggt tgcgccgcaa tcgccagctc
     2521 tgtgccctgc aattaaatga aaagcgcgtc gatgtggtgt atgccaaaga ggtggatttt
     2581 tcacgagccc ccaacctgtt tggcctgttt gccaataggc gcgcagcttt gcaagcattg
     2641 cagagcatcg ctgatgaaca aaaactttgt tatggcctgt tgggactgga accgttaagt
     2701 cgcggtcgtg catgttttcg ttcagcgcta aaacgttgcg ccggagcatg ctgcggtaaa
     2761 gagagccatg aggaacatgc gctacgcttg cgccagtctc tggagcgttt gcgggtggtg
     2821 tgttggcctt ggcaaggtgc ggtggcactg aaagaacagc acccggaaat gactcaatat
     2881 catattattc aaaactggct gtggctgggg gcggttaatt cgctggaaga agcgacaacg
     2941 ttaattcgga cacccgccgg gtttgatcac gacggttata aaattctttg taagccgctg
     3001 ctttccggta actatgaaat tactgaactt gatccggcga atgaccagcg agccagttga
     3061 tttcactgaa caacagttta ccttgcggtt gtaaaagccg cagggtgtgc ctgccatcga
     3121 accagcacgc gccctggtcg gtagtgagca atttatcgcc cagttgccac gccccgttaa
     3181 tcacaaacac caccccgccg cgtgagccaa aggtagtaaa cgtacgttcg gcaattctga
     3241 ctttcgcctt acagacatcc aggcgtgtca tgatgttgaa atccatcgac atctgccctg
     3301 ccgtcagttt cgcttttacc acctggtccg ctgcaaaggc aaaaggctgt aacggtttta
     3361 aggtatggtt aaagcggtct gcgctttcaa ggaacatctc gccgccttcc agcaacgtca
     3421 ctatcctttc catgccggga aacaaagaaa actccccatt cgccgcgatg gatgcaatgc
     3481 tggcacgcca gtaaaaatca cgttttgccg gtgggaaagt gcaaatttcg cgcgtttcac
     3541 cggcagcgtt tcgccacaga ttcaccgaca ttttacgcat atcaaagtat tccatactcg
     3601 ctcccggccc gttcttttca ttgttatcgt tcaggtaacc gtggatggtt atcggcaact
     3661 cgggtgattt acgttagtgg tgatcaggct ttttttacaa ctgtttgaga agagaaaaga
     3721 aaaccgccga tcctgtccac cgcattactg caaggtagtg gacaagaccg gcggtcttaa
     3781 gttttttggc tgaaagatta ttcagcagtt gcaggcattt taccttttgc cgctggacgt
     3841 tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg cgtcaggatg
     3901 ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg ctgttcttcc
     3961 atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc aatgatgtca
     4021 tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg gcctttcatg
     4081 atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa catcatgtcc
     4141 tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc cggtgctgcg
     4201 gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt agaggcaaca
     4261 aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt attacggctt
     4321 tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt caattaatca
     4381 gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat ggagaaaaaa
     4441 agaaaatttg atggagagtg atgagagaat aatacaacac gatgattttg cagagattat
     4501 gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt aatctgtatt
     4561 aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag tattagctta
     4621 ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta gggaataaca
     4681 catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc agcaatgttc
     4741 ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg aaggacggcg
     4801 agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc acgataccga
     4861 cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac tggcgatgat
     4921 cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc gcgccaaaat
     4981 gtctggcgct gaaatgggta aacgtaccac caggttcctg atggaacacc agcgcctcca
     5041 gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg cgttgcggta
     5101 atacaccgaa ctgcggatgc aaggagccac gaattgcggt atgtagatca aggtgccagc
     5161 gcacagattc tttgcccagg tcataaaaat cttccaggca ctgttccagt tcgcgcgccc
     5221 gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt cgattcatat
     5281 cgctatggca ataacgtttc ccttgcttca gcgcaggagg attcccgagg atcaccagca
     5341 accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg tccagcatct
     5401 ccacaggtgc cgtctcatta ccgtgtattc ccgctgaaat caccagtgcg ccttgcggtg
     5461 gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga acgccgttga
     5521 tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga aaattatcca
     5581 ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa ttggctcaat
     5641 acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc ggcagcagta
     5701 aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag cgtatcgttc
     5761 atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaataccac ccgcaaccgc
     5821 aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac ttttagttcg
     5881 ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc ggcgtgttcc
     5941 cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa cagttggctg
     6001 ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg aacttctatc
     6061 gccataaaac cgttcacccg cgcacgcagg tttgccagta actgtgactg gcgagcgaac
     6121 gcctgttggt ggcaaaacag cacctggcgg ttactcacgg caatcacgtc attatgaaaa
     6181 acgccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg gggattcacc
     6241 tgattcagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg ataacgggaa
     6301 ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc cggctcaccg
     6361 taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa cgctacctgc
     6421 ggcaacgccg aatgaacgct aaatttctct tcgtcgttaa aaatcgcttt taaaagtgat
     6481 tcggtgacgt gcgcttccag cgagcggtga aatttattgt tcaggttggc aacggtgaga
     6541 tgcactttgc catccagcgt atcggcagat ggcgcgatcg ttgccgcatt ggctacccac
     6601 attggcgaag cggaactgac gctggaaagc cagtgcggtg cctggcgtgc aactttttcc
     6661 agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat aaacggacgc
     6721 tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggcttt cattttcagt
     6781 aagccctgtt ttgccgcctg tcgcgggtta gacacctgaa aacggtgacg ggtagaggct
     6841 tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc gtcgaaatta
     6901 acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg ttaagcgttg
     6961 cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag taatctgcgg
     7021 cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat ggcgcggtac
     7081 tggcagcacc agtaagcggt ttgttccagt taacaatccc cgcccgcgcc tccagcaaca
     7141 gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg aagcgagtgt
     7201 tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc aataacggtc
     7261 cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc cccggcgtca
     7321 gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg cccatcgctt
     7381 ccagttgctg ccaggcagta accacctgct gtgcggcctg ttcagaaatc agcccgccaa
     7441 taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg acggcaacca
     7501 gacgagcaag aaacgcatcg ccctgcgctc cgcttttgag caataaacgg cgggcgcagg
     7561 tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca gccgcgtcga
     7621 tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg gcgagaattt
     7681 tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg ctaccggtaa
     7741 acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca cgcccgccct
     7801 gcaccaggtt cagtacgccc ggcggcaagc cagcctgctg ccataaacgc attaccgctt
     7861 cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct gccagcaatg
     7921 ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca aacaccgcca
     7981 gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca ctacgctgct
     8041 cgccggtacg agcgtgatac gccttaattg atatcgcgat tttattgatc atcgccgtca
     8101 cttcggttgc cgcttcccag cgcggcttgc cggtttctcg ggcaatgatt gcggttaatt
     8161 cgcctttatt cctttccagc aatccggcaa agcgttcgac aacgacctga cgttcagcca
     8221 atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc tgctcgacct
     8281 gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga ttacgcttca
     8341 cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc atgctgtttt
     8401 ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt tgagggcatc
     8461 cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg ccagcaccac
     8521 gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc cctgcgcagg
     8581 ctgcccttct gccacttcca ccagccgact tttacgaatg gcgcgcacgc ggtcgatgtc
     8641 acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac gaaaaccttc
     8701 tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga tgacgtcctg
     8761 ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt ctgcaataaa
     8821 tgccttttgc ccggtgccgc agagaaaatc ggcgcggcta aaatccatcg aaaagaagcg
     8881 tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc cgcgcatttc
     8941 agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa agcgcgattt
     9001 cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca gcgtgcacag
     9061 ctcgctgctg ccggtgtgat cgttactgag aaacagcgtc ggcaatgcgt tatagacatt
     9121 cagctctttt gaggcgtgaa ccaacgtgcc gacgcgatag ttgtaccagg gatcgttcag
     9181 cccaaccgcc acctcaatgg cacaaatccc cgccacggtg cctgtctcgc tatcttccag
     9241 cacgaacaca tagccctgct cacttttggg cagttcgcct tgccaggttt tgattgccct
     9301 ttcgatacgc gccgaaagcg tggcttcatt ggcgggaagc gacgtcaggc cgccgcccgt
     9361 tttgctggca agctgcatca gcgccgagac atctgatcgc tcaacgggac ggatgaccat
     9421 catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc cagtccggtc
     9481 gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt gccacccgca
     9541 atcagtacca tcacgcctgc tttcgccgct tcctgagaga tctgtttcgc ttgcccggcg
     9601 taatcggcat tcagtacaca gccaatcagc aaacctaagc cgcgaacttc actgaacaaa
     9661 ccatagcggt gattaatagt attaagacgc tcaacaaacc agtcgtgacg ctgtttaacg
     9721 ccattaagca tctctggtgt gttgatgagc tccagcactt tgcctgccac cgccgaggcc
     9781 agcggattac cgccataggt ggtgccatga gtgccaacgg tcatcacgcg ggcgcactct
     9841 tcggttgcca acaacgcacc gaccgggaaa ccgccgccca gcgctttggc ggtagttaac
     9901 agatcaggcg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg cccgacgccg
     9961 gtttgtactt catcaaaaat caacagcgca ttgtggcggt tacacaattc acgcagacct
    10021 tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg ttcgacaatc
    10081 accgcacagg tagagtcgtc aatcagcgcg ctggcagagt taatatcgtt atatgcagca
    10141 tgacgaatat ccgccggcag tggcgcaaaa tcctgtgaat aggctggctg cccacccgca
    10201 ctgacagtaa acagcgtgcg accatgaaac gcatttttga acgccacgat gccgctctta
    10261 tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc ttcgttggct
    10321 tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat caattttttc
    10381 gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa cttactcgcc
    10441 tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt caccgcaatg
    10501 ccacccgcga agtcgatata ctctttcccc tgctgatccc acaagcgcga accttcgcca
    10561 cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc aaagttttca
    10621 cgcgtaattg gctgagacat agcgacctct acagtaaata attcgttatt tatatattaa
    10681 caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc cagtgataaa
    10741 agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac catatgcatt
    10801 taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa taaaaaacgc
    10861 aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa atttactgga
    10921 aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg cgccatcgtt
    10981 gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca acgcgaaatt
    11041 ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa atttgtctct
    11101 tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt cgaaaagcac
    11161 caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt tccgctcgaa
    11221 gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca ttatggcgtg
    11281 gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg tgacgacgaa
    11341 gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa tgtcaccgtg
    11401 atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt cccggcaaaa
    11461 gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg tgataatccg
    11521 gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg cattggcgaa
    11581 gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga agagcgcgaa
    11641 tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc taatccgcaa
    11701 acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga taaccgtggt
    11761 ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt agaaaccggc
    11821 atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt ctgggcgacc
    11881 ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat taaccttgag
    11941 aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta taccgtcact
    12001 gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa actccacgat
    12061 gaacgctgag cgtaactttc tgtttgcctg tcttattttt gcgctggtca tttacgctat
    12121 ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga cactcattcg
    12181 ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg ccaccctctt
    12241 tctgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc cgctcttagg
    12301 gacactactc tcattaattg ccgcaacgct ggcctcctcg tgctcattcc tgctggcgcg
    12361 ctggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct tccaggccat
    12421 tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct taatcccgtt
    12481 gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct tctggcctta
    12541 tacccttatt tcggcactca cgaccctgcc cggtattgtt atttataccg tgatggcaag
    12601 cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc tggcgggcct
    12661 ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac atgtggatct
    12721 gtctgcttcg cgccgcagcc cacttactca cccaaaaaat gaaggataga acgatgtcgc
    12781 aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg gctgttgccg
    12841 ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac gacggacttt
    12901 cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg ctggtgcaaa
    12961 ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa gatggctcca
    13021 acggacataa atcgctgttt atggcttatg cgaccccgaa agcattttat gaagccctga
    13081 aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa acgactcatg
    13141 tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa gcgtattcct
    13201 tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt ggcggtaatt
    13261 taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc tgcccggtcg
    13321 gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc gaagtgaaat
    13381 tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt acctttaaaa
    13441 tcaccgaata aagccaggat aaaggatgat gaagatacaa tcgcgtaaaa tctggtacta
    13501 ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc tgcttcccgg
    13561 cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc aacagggtat
    13621 agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct tgttgatgat
    13681 tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca atgcgtcgct
    13741 gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg gcgcggcgct
    13801 gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa ccggcaaaac
    13861 cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca tcctggtctg
    13921 tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccggtc tgacttcaat
    13981 acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccggcga ctattgttta
    14041 ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg gactgtttat
    14101 tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg agcgccagaa
    14161 gaggaatgcc tgatgggttt accgccgctt agcaaaattc ctttcatttt acgtccgcag
    14221 gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg gtgggggcgg
    14281 atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg caaacgctca
    14341 ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat gtgcctgtgt
    14401 gagttttgcg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag caccgataaa
    14461 ctgctggcag tggctgactg gcggcaaagc ccgctcttca gcgatgaaga acggctggcg
    14521 ctggagtacg ccgaagccgc aagcgtaacg ccgccaacgg tcgatgatgc cctgcgcacc
    14581 cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt gatcggcctg
    14641 caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg gctgtgccgt
    14701 attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt gctgggattg
    14761 ttatcgctgt tttctctggc aacacatgcc agtgactggc aggaaattaa aaatgaggcc
    14821 aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa ccgctatctc
    14881 gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat tgttcgtctg
    14941 gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg acgtaaaacg
    15001 ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt aaaagaggcc
    15061 aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt cgacacacag
    15121 ctgccggtgc gggaagattt ttcagtgcct acagaagggg ctgaatcgcc ctggggcggc
    15181 gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca aacgccgcaa
    15241 gccttactgg agtttgctaa agccaatccc ggcacggtta cctatccgcg cccaccggac
    15301 tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgcccga tcccgccgcg
    15361 ttaaaagttg ccccggataa agccactttc gcccgcgtca ctgcgccctt gtggcaatat
    15421 cttgacgcgc tacatcccta tttatggcgc aaaggaaagg atttcccgcc ttcgcccgcg
    15481 cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt taaccctgct
    15541 catgcccagc aaaaaatcgc cagcggcgat ttgcctgcaa gcagttacag ttttggtttt
    15601 aaccagggga tgattggcaa cgtgcatttc gtcaccattc ctgctaacgc aaatgccagt
    15661 gctgcggcga aggtggttgc caacttcctg ctctcaccca atgcgcaact gcgtaaagcg
    15721 gatcccgctg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc tgatgggcag
    15781 cgcgaaacat tgcaatcaag aatgccgcaa gatctgccgc cggtactggc tgaaccgcac
    15841 gcaggttggg taaatgcgct ggaacaagaa tggctacacc gttacggtac gcattaatct
    15901 ttttgctgtg ggcgatggtg gcggtgattt atgcaccact gatcccggca gctctcacgc
    15961 tgatatcgcc tgctttgtcg ttgacacact ggcaggcgtt atttgccgat ccacagttac
    16021 cgcaggcatt actggcaacg ctggtgtcga caaccatcgc ggcggtcggg gcgttgttga
    16081 ttgccctgct ggtgattgtg gcgctgtggc cttggccgaa atggcagcgt atgtgtgccc
    16141 gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc cttctgctct
    16201 ttgctgaggg agggctgctt tatgactatt tcccgtattt cactccgcta atggacaaat
    16261 tgggtattgg gctgggcctc acccttgcag tgaaagaaag cgcatttctg ctgtggatct
    16321 tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg gattcgctgg
    16381 gctacagccg ctggcaatgc ctgaactggc tgctcttgcc ctccgtcgca cctgcactgg
    16441 caatggcgat gttggcgatt gttgcctggt cgctttcggt cgtggatgtg gcgattattc
    16501 ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta actcagggcg
    16561 acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg ttactcgccg
    16621 cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt ccccgcgtag
    16681 atggcattcg caagcctgcc acacctttat taccaggcac tacgctggcg agttttttac
    16741 ccttaaccgg tgtgctgtgt gtggttctgc tggcgatcct cgcggatcag tcgacgatca
    16801 atagtgaagc actcatcaac agcctgacaa tggggctggt ggcgacattc atcgctttgc
    16861 tcctgttact gctgtggctg gaatgggggc cacagcgtcg ccagttgtgg ctctggttgc
    16921 ccattctatt gcccgctctg ccactggtgg cgggccagta cacgctggcg ctatggctga
    16981 acctggatgg aagctggacg gcggtggtct gggggcatct gctgtgggta atgccgtgga
    17041 tgctgtttat cctgcaaccc gcctggcagc gcattgattc acggttaatt ttgattgcgc
    17101 aaacactgag ctggtcgcgg gccaaaatct tcttttacgt gaaatgtccg cttatgctac
    17161 gccctacgct gattgccttc gcggtgggat tttcagtcag tattgcgcag tatatgccga
    17221 cgctgtggct gggcgcgggg cgttttccga cgctcaccac tgaggcggtg gcattaagca
    17281 gcggcggcag caatggtatt ttcgccgccc aggctttatg gcaactgcta ttaccgctta
    17341 ttatttttgc cctgaccgcg ttagtcgcaa aatgggtagg ttatgtcaga caaggactcc
    17401 gctaatgctc tgcgtgaaaa atgtttcgct acgtttacca gaaagccgct tgctgacaaa
    17461 cgttaacttt acggtggata aaggtgacat tgtcacgtta atggggccgt ctggctgtgg
    17521 aaaatccact ctgttttcat ggatgattgg tgcactggct ggacagtttt cttgtacagg
    17581 tgagctatgg ctcaatgagc aacggattga catgctaccc accgcacagc gtcagattgg
    17641 cattcttttc caggatgcac tgttatttga ccagttcagc gtcgggcaaa atttactgct
    17701 ggcgctaccg tcgacgctca aggggactgc caggcgcaat gccgtgaaag acgcgcttga
    17761 tcgagcggga ctggctgaaa cttatcacca ggaccctgcg acattatcag gcggtcagcg
    17821 agcgcgggtg gccctgctgc gcgcccttct cgctcagcca aaagcgttac tcctggatga
    17881 gccattcagc cgtcttgatg tggctctgcg cgataatttt cgccagtggg tgttcagcga
    17941 agttcgcgaa ctggcgatcc ccgtcgttca ggtaacgcac gatctccagg atgttcctgc
    18001 tgatagttct gttctggata tggcgcagtg gtcagaaaat tacaacaaac tgcgataacg
    18061 caaagttttt ctcaatgcgt cagttcagaa tggcgcactc aaaactacaa tgtcgggatt
    18121 ttcgatgaaa cgtgtttctc aaatgaccgc gctggcaatg gctttagggc tggcttgcgc
    18181 ttcttcgtgg gccgctgaac tggcgaagcc tcttacacct gaccagcttc aacaacaaaa
    18241 tggcaaagcg atagatactc gccccagcgc gttttataac ggctggccac aaaccttaaa
    18301 tggcccttct ggtcatgaac ctgccgcctt aaacctctct gccagctggc tcgacaaaat
    18361 gagcaccgaa cagctcaacg cgtggatcaa gcaacataac ctgaaagccg atgccccggt
    18421 ggcgctgtac ggtaatgaca aagatgtcga cgccgtcaaa acgcgactgc aaaaagcagg
    18481 ttttacgcat atctccattc tgagtgacgc gctaagcgaa ccttcccgtc tacaaaaact
    18541 gccgcacttt gagcagctgg tttatccgca atggctgcac gacctgcaac aaggtaaaga
    18601 ggttacggcg aaacctgccg gtgactggaa agtcattgaa gcggcctggg gcgctcctaa
    18661 gctttacctt atcagccata ttcccggtgc tgactacatc gataccaacg aagtggaaag
    18721 cgaaccgctg tggaacaaag tttctgatga acaactaaaa gcgatgctgg caaaacacgg
    18781 cattcgccat gacaccacgg tcattctgta cgggcgtgac gtatacgctg gagcgcgtgt
    18841 ggcgcaaatt atgctctacg caggcgtgaa agatgtgcgt ctgctggatg gcggctggca
    18901 aacctggtcc gacgcgggac tgcctgttga gcgaggaacg ccaccgaaag tgaaagcgga
    18961 accggatttc ggcgtgaaga tcccggcaca accgcagttg atgctcgata tggaacaggc
    19021 ccgtggactg ctgcatcgcc aggatgcatc gctggtgagc attcgttcgt ggccagaatt
    19081 tatcggtacg accagcggtt acagctatat taaaccaaaa ggtgaaatag ccggagcacg
    19141 ttggggacac gctggtagcg actcgacgca tatggaagat tttcataacc cggatggcac
    19201 catgcgcagc gccgatgata ttaccgctat gtggaaagcg tggaatatca aaccagagca
    19261 gcaagtttca ttctactgcg gcaccggctg gcgcgcgtcc gaaaccttta tgtacgcacg
    19321 cgccatgggt tggaagaatg tttccgtgta tgacggcggc tggtacgaat ggagcagcga
    19381 tccaaaaaat ccggtagcaa ccggtgaacg cggcccggac agtagcaaat aacattgcaa
    19441 ttactgacgc tggtgcgact tcagcgtcag atacccactc cacacccgcg taaaggtcgt
    19501 catccagcac aacgcaccga agatccacgc aaaccacgca aaccatgcag ggaataaaca
    19561 tcccagcaca aacagtaaga tcgtttctgt gccttcggtt aacccgccca gataataaaa
    19621 cgatttatgc gcataaccgg gattatcaat ctgatgtttc gccgccagcg cagcaaaagc
    19681 gagaaaactg ctgcctgtgc cgataaacgc aaacagcagc cagccgcctg ccagggcgtt
    19741 ttgctgcggt gcggcgagga taaacccaaa aggtaccagc gcgtaaaaaa gaaaatcgag
    19801 agaaatatca agaaagccgc ccgcgtcggt aagccctctg cgccgcgcca gcgcaccgtc
    19861 cagaccatca agcagcctgt tcaacaaaat gacgacaagc gccgccagat accagcccag
    19921 cgccagaaaa ggcagtgcca gcacgccgat ggcaaatccc actaatgtta aaccatctgg
    19981 cgtaatgccc ggtttatcaa gaacccgcac gcactgatgc aacaacggtt taatccgggg
    20041 atgaagatgg cggtctagca caggcactcc ttaaatataa agcctttctg attgagcaac
    20101 agtgcggata ttatggcatt tttcgcttat ctgcccgtgt gtaatttatg aaaatgattg
    20161 aagttgttgc cgccatcatt gaacgtgatg gcaaaatttt actcgcgcaa cgccccgccc
    20221 agagcgatca ggcgggatta tgggagtttg ccggtggtaa agtcgagccg gatgaaagcc
    20281 agcggcaggc gctggtgcgt gagttacgcg aagaactggg catcgaagca actgtgggtg
    20341 agtatgttac cagccatcag cgagaagttt cgggccggat tatccatctt catgcctggc
    20401 acgtacccga cttccacggg acgttacagg cacatgaaca tcaggcgctg gtctggtgct
    20461 cacctgaaga ggcgctgcaa tatccgctgg cccctgctga cattccatta ttagaggcgt
    20521 ttatggcttt acgcgccgcc agaccagcgg attagtgcta agggttttgt catcccgctg
    20581 gcattgcagc agtattccct cggctttaat taccgcgcct tcagaataat ttttatcctg
    20641 ataaacgcag cactgagtac agggctgcgc tgactgcccg cctgaactga acacttctgg
    20701 cggtacgttt acctccacgt ccggacgata atgcgggtta gccagtgcaa ttaatggaaa
    20761 tgctaatact acggcgaaca atgctcgact cacagggaac tccttaacgt tattgtctct
    20821 gctactgata acggtagccg ggtggcaaaa ctttagcgtc tgagttatcg catttggtta
    20881 tgagattact ctcgttatta atttgctttc ctgggtcatt tttttcttgc ttaccgtcac
    20941 attcttgatg gtatagtcga aaactgcaaa agcacatcac ataaacaaca taagcacaat
    21001 cgtattaata tataagggtt ttatatctat ggatcagaca tattctctgg agtcattcct
    21061 caaccatgtc caaaagcgcg acccgaatca aaccgagttc gcgcaagccg ttcgtgaagt
    21121 aatgaccaca ctctggcctt ttcttgaaca aaatccaaaa tatcgccaga tgtcattact
    21181 ggagcgtctg gttgaaccgg agcgcgtgat ccagtttcgc gtggtatggg ttgatgatcg
    21241 caaccaggta caggtcaacc gtgcatggcg tgtgcagttc agctcagcca tcggcccgta
    21301 caaaggcggt atgcgcttcc atccgtcagt taacctctcc attctcaaat tcctcggctt
    21361 cgaacaaacc ttcaaaaatg ccctgactac cctgccgatg ggcggtggta aaggcggtag
    21421 cgattttgac ccgaaaggta aaagcgaagg cgaagtgatg cgtttctgcc aggcgctgat
    21481 gaccgagctt tatcgtcatc tgggcgcgga taccgacgtt ccggcaggtg atatcggcgt
    21541 tggtggtcgt gaagtcggct ttatggcggg gatgatgaaa aagctctcca acaataccgc
    21601 ctgcgtcttc accggtaagg gcctttcatt tggcggtagt cttattcgcc cggaagctac
    21661 cggctacggt ctggtttatt tcacagaagc aatgttaaaa cgccacggta tgggttttga
    21721 agggatgcgc gtttccgttt ctggctccgg caacgtcgcc cagtacgcta tcgaaaaagc
    21781 gatggaattt ggtgctcgtg tgatcactgc gtcagactcc agcggcactg tagttgatga
    21841 aagcggattc acgaaagaga aactggcacg tcttatcgaa atcaaagcca gccgcgatgg
    21901 tcgagtggca gattacgcca aagaatttgg tctggtctat ctcgaaggcc aacagccgtg
    21961 gtctgtaccg gttgatatcg ccctgccttg cgccacccag aacgaactgg atgttgacgc
    22021 cgcgcatcag cttatcgcca atggcgttaa agccgtcgcc gaaggggcaa atatgccgac
    22081 caccatcgaa gcgactgaac tgttccagca ggcaggcgta ctgtttgcac cgggtaaagc
    22141 ggctaatgct ggcggtgtag caacgtcagg ccttgaaatg gcgcaaaacg ctgcgcgcct
    22201 gggctggaaa gccgagaaag ttgacgcacg tttgcatcac atcatgctgg atatccacca
    22261 tgcctgtgtt gagcatggtg gtgaaggtga gcaaaccaac tacgtgcagg gcgcgaacat
    22321 tgccggtttt gtgaaggttg ccgatgcgat gctggcgcag ggtgtgattt aagttgtaaa
    22381 tgcctgatgg cgctacgctt atcaggccta caaatgggca caattcattg cagttacgct
    22441 ctaatgtagg ccgggcaagc gcagcgcccc cggcaaaatt tcaggcgttt atgagtattt
    22501 aacggatgat gctccccagg gaacatttct tatgggccaa cggcatttct tactgtagtg
    22561 ctcccaaaac tgcttgtcgt aacgataaca cgcttcaagt tcagcatccg ttaactttct
    22621 gcgatagcag cagatatgcc agtaaagaaa tcccattaga ctattttttt gataatcttc
    22681 ttcgctttcg aacaactcgt gcgcctttcg agaagcaagc attatataat gccaggccag
    22741 ttcttcttca attgtcccgt tttgaaaagc tgtgcttgat atcgagatca tccatgataa
    22801 ttccgccgcc catattagct tcgccgagga tttaccggag cgattattag cgcaatcaga
    22861 gatatagtct gagggaaaaa cagcaaattt attcaacaag gcgataacct gctctggggc
    22921 ttcctccatg tttgctttaa aggtattggc tccatggtcg ccagaaagaa aatgctccat
    22981 taaggcacaa taactttcgc tatcttcgat accccattga tcctctaaag actcgcgtct
    23041 tttacttatg atatcgatcg agtcaaaagg aagcacatga tattggaagg tatctttgcc
    23101 aggttcaggc tttcgcggcc agaactccag cgtttcagac cattgcttat gatagaatcg
    23161 ataaggtgcg atcaattgta gcgcctgtaa cttctcgata ctgagcggct caataccttt
    23221 agcctgataa taatgcagtt gttctttttt tgctttaaaa ccggcccgaa caataagccc
    23281 catcataatt aatagataaa gaaaagagca tcccgcagta atcaggcctc tttcattcaa
    23341 accgttggat gttatcgctg cgaatacaaa cattacagcg acaacacatg ttaaataccc
    23401 cccccccact tacaaagcag catggcctta ttttctttaa tcatccgttc aaaattacta
    23461 ttaaatattt cccagccatt aaaagaatac ttctcgctcc caggatggtt ttgtaataaa
    23521 acttttttca tcacattccc tgttattaca tactttagag gcgataattt tatccatgca
    23581 aaaaaaatat ccaacgaatt ttcttgatcc gctggatatt tcagaatatg actcgaatag
    23641 cacgaaagat tcgctcgctt acgctatcgc cccgcttccg gcttcatctg ctggcggact
    23701 ttttttcgca ctacgtttac gcggtgcagc ctttttctta tcagcactgc caccactgcc
    23761 cggagccaca atgccgcgaa actgccgcac cggagtacgt ttggcttgat caataagctg
    23821 atatagcgtc cccaccagcg gctgcataaa gtcctgatag cgacactgct tttcgctgat
    23881 ttgcgtcagc accgattccc agtgcgcggt catgtccggt cgcgtagcca tttctggcag
    23941 cgaatggaat aacgcttttc cggcgtcggt ggagtggata tagcgccctt ttttggtgag
    24001 gaaaccacgc ttgaacaaca gttcaataat cccggcacgc gttgcttctg tccccagacc
    24061 atcggtcgca cgaaggatct ttttcagatc tttgtcctgc acaaagcgcg caatcccggt
    24121 catcgccgaa agcagtgttg catcggtaaa atggcgcggc ggctgggttt gccgctctac
    24181 cacttcacct ttttcacaca gcaactcatc gcctttcgcc accacaggca atggcgtgcc
    24241 gtcgttttct tcatcgcgct ctttgctgcc taacagcgcg cgccaacctg cttcagcaag
    24301 aaaacgcgct ttagcgacaa atttgccttt ggcaatgtcc agttcgataa cacacttgcg
    24361 gaacaccgca tccgggcaga actgcatcag atactgacgg gcaatcaggt tatagacctt
    24421 cgcttcgttc tccgtcaggt tgatcgcaga actccttgcg gtcggaatga tggcgtggtg
    24481 cgcatcgacc tttttgtcat cccaacagcg gttgcgtata tctggatcta ccactgactg
    24541 cggcaacaga tccggtgcat gaacgctgat ggcattcatc accgcgtggc gtccggcaaa
    24601 atgttcttct ggcaaatagc gacaatcgga acgcggataa gtgattagct tgtgcgtttc
    24661 gtacagcttc tggcagatat caagcacgtt ctgcgcgctc agaccgaagc gttttgccgc
    24721 ttcaatctgc aacgccgaaa gcgaaaacgg cagcggcgcg gattctgatt cccgtttatc
    24781 gttatagctg gtgacaatag ccggttgacc actaatgcgg ttaaccacat gctccgccag
    24841 tggacgatgt aacaagcgcc cttcttcatc ctggtacggt tcacacgctt cgctcggttg
    24901 ccagatagcg gtaaaccgct catcggcagg tgtcacgata tgcgctttga cttcaaagaa
    24961 gtctttcgcc acgaagtttt caatctcttc atcgcggcgc accaccagcc cgagcacggg
    25021 tgtctgcacg cgtcccacgg aaagtacgcc ctgataaccg gcattgcgac cgagaatggt
    25081 atacgcacgg gtcatattga tgccgtacag ccagtcggca cgcgctcgcg ccagcgcaga
    25141 aacgcacagc ggtacaaact cactgttgga acgaagacgg tcgatcgccc gctcaaccgc
    25201 ctgcgggttc aggtcgttta tcaagcaacg ctgcacctgc tggcgctttt ccggtgccag
    25261 ttgtagatag tccagcactt catccaccag cagttgccct tcgcgatccg ggtccccggc
    25321 gtgaacgatt tcgctggctt catgcaggaa ccgtttgatg acgttaagtt gtttggtcac
    25381 ggagggtcgg ggctgtaatt gccacttttc cgggacaatc ggcaaatccg caagattcca
    25441 gcgcgcatag cggctgtcgt aggcgtctgg ctgcgcctgc tcaagcaggt gaccgataca
    25501 ccaggtcacc acctgaccat taccgcactc gataaagcca tcgcctttac ggtgcggttt
    25561 gggcaggaca tcagcaatgg cgcgcgccag actcggtttt tcggcaataa acaaccgcat
    25621 tgaattaacg gatctcaacc atggcacgac cgccgcgcgc tggcaccagt tcgccaatcg
    25681 ccgtcagttc aatgccaaac tcggcggctg tagctttaac ctcattttct gcttccggca
    25741 tgaccgccag cagcaaaccg ccagaagttt gcggatcgca cagcagatcg cgcacttcac
    25801 gcggcatttc acccatcaga tgaccgtagc tggcaaagtt acgttcggtg ccgcctggca
    25861 ctgcgcccaa cttaatgtac tcttcaaccc ccggcaattt cgggatcgcg tcatagtcaa
    25921 cgcgcgcctg cacacctgcg ccctgacaca tttcgctcaa atggcccagc agaccaaagc
    25981 ccgtaacgtc ggtcatcgct tttacgcctt cgatgttggc aaaggacgcg cctgcgatat
    26041 tcatccggca catcacttct gtcgccagtc cctgatgttc tggtttcagc agtgattttt
    26101 tctcagccgt ggtaagaacg ccgatcccca gcggtttcgt caggaacagt ttgcatccgg
    26161 cttgtgcggt actgtttttc ttcacccgct cggtcggtac gatccccgtt accgccagac
    26221 caaaaatcgg ctccggcgca tcgatggagt gaccgcccgc cagcgcaata cccgcctgac
    26281 ggcatgcata gcgtccacct tcggttactt cgcgggcaat ttccggggaa agtttgttaa
    26341 tcggccagcc gaggatcgca atcgccataa tcggtttgcc ccccatcgcg aagatatcgc
    26401 tgatggcgtt agtcgccgca atgcggccaa aatcgaaagg attatcaacg atcggcataa
    26461 agaagtcggt ggtactgata acgctggtgc cattgcccag atcgtacacc gccgcatcgt
    26521 cgcgggtctc attacccaca agcaaattcg gatcaacaaa cttcgcctgc tcactgtgca
    26581 ggatggtttc caacactttt ggggaaattt tacagccgca accagctccg tggctgtatt
    26641 gggtcaaacg aatcgagttc tcgctcatgg acatctcctg tcaatgcaat ccgggtatgg
    26701 taaccctcat tccgtgaagt gataagtgag agtgtctgaa ttcctgcgcc tttgctcaca
    26761 atccagacag tttcgcgaca attatcagaa ataagttaca aacggcgtcg ggtccgggac
    26821 gttaatcgac gtagatgctt tcaactgcgg cgtgccgagg tagagaaaac cgacaatttt
    26881 atcctgctca cggcaaccga atgcttcacg cactaccgga ctttcagtta atgcgccact
    26941 gcgccagatg ccgccaaatc cttgggcaac ggctgccatt tgcatcgcca tgaccgcgca
    27001 tccggcagac atttcctgtt cccatcgcgg gactttatga ttttcttcgc atttcgccac
    27061 caccgtgatg atgagcggtg cgcggaacgg cgcattacgg gctttgtcga tagctttgtc
    27121 atcactaccg gcagcaatcg ccccctgttc cagtacggcg ctgaaacgct cgcgcccttc
    27181 cccttcaatc acaaaaaaat gccacggttg catggactta tggtccggcg cacgcatacc
    27241 cgcacgcagg atgttttgca gttgttcacc cgttggcgcg ggttcagcca ggcgggaggc
    27301 gctacggcga ttgatcaata gttcgagtgc atccatttga ttaactcctg tcgtgatatt
    27361 tattcacaaa attaacacga gagtggattt tgttacagca cagtccgcaa ttcctgctga
    27421 caagtgccgg ttgggtcatt acgataacca catctattgc gcctgtgaca ggtgtgacct
    27481 taagttggga gaatacatgc gaaccctttg gcgatttatt gccggatttt ttaaatggac
    27541 gtggcgtctg ctgaatttcg tccgtgaaat ggtacttaac ctgttcttta ttttcctcgt
    27601 actggttggt gtggggatct ggatgcaggt cagtggtggt gattcgaaag aaacggccag
    27661 tcgtggcgca ctgctgctgg acatttctgg tgtgatcgtc gataaacccg acagttctca
    27721 gcggtttagt aaattaagcc gccagctgct tggtgccagt tccgatcgtc tgcaggaaaa
    27781 ctcactgttt gatatcgtca acaccattcg ccaggcgaag gacgaccgca atatcaccgg
    27841 tatcgtgatg gatctgaaaa acttcgcagg cggcgaccag ccgtctatgc agtacatcgg
    27901 caaagccctg aaagagtttc gtgacagcgg gaaaccggtt tatgccgttg gcgagaacta
    27961 cagccagggg caatattatc tcgccagttt cgccaataaa atttggctgt caccgcaagg
    28021 cgtggttgat ctgcacggtt ttgctaccaa cggtctgtac tacaaatcgt tgctggataa
    28081 gctgaaagtt tccacccatg tgttccgcgt gggtacgtat aaatctgccg ttgaaccgtt
    28141 tattcgtgat gatatgtcac cggcagcccg cgaagctgac agccgctgga ttggtgagct
    28201 gtggcaaaac tatctgaata ctgttgccgc taaccggcag atccctgctc agcaggtatt
    28261 ccctggcgcg caagggttgc ttgagggttt aaccaaaacc ggtggcgata ccgcgaaata
    28321 tgcactggaa aacaagctgg tcgatgcact ggcatcgagt gccgaaatcg aaaaagctct
    28381 gaccaaagag ttcggctgga gtaagactga taaaaattat cgcgccatca gttattacga
    28441 ttacgcattg aaaacgccgg cagataccgg tgacagcatc ggtgtcgtct tcgctaatgg
    28501 cgcaattatg gatggcgagg aaactcaggg gaatgttggc ggtgatacca ctgcggcaca
    28561 aatccgcgac gctcgccttg acccgaaagt gaaagcgatt gtcctgcgtg ttaatagccc
    28621 aggcggcagc gttaccgcgt ctgaagtgat tcgcgctgaa ctggcagcag cccgggcagc
    28681 gggtaagcct gtggttgtat cgatgggcgg catggcggca tctggtggtt actggatttc
    28741 cacgccagct aattacattg tggctaaccc cagcaccctg accggttcta tcggtatctt
    28801 cggcgtgatc accaccgtag aaaatagtct ggattcgatt ggtgttcata ctgatggtgt
    28861 ctcaacttca ccgctggcgg atgtttctat caccagggca ctgccgccgg aagcgcagca
    28921 gatgatgcaa ttaagcattg agaatggcta taaacgcttt atcacgctgg ttgctgatgc
    28981 gcgtcattcg acgccggagc aaattgataa aatcgcccag ggccacgtct ggaccggtca
    29041 ggatgcaaaa gctaacgggc tggtcgatag tctcggggat ttcgatgatg cggttgccaa
    29101 agcagcagag ctggcaaaag tgaaacagtg gcatctggaa tactacgttg atgaaccgac
    29161 cttcttcgac aaagtgatgg acaacatgtc tggttctgtc cgggcaatgt tgccagatgc
    29221 gttccaggcc atgttacctg caccgcttgc ctcggtagcc tctaccgtta aaagtgaaag
    29281 cgacaagctg gccgcgttta acgacccaca aaaccgttat gcgttttgcc tgacctgcgc
    29341 caacgtgcgt taagtcttgt actgagtggc cgacagatcg tcggccacat tattttttac
    29401 ctcgacgaat cctcttcccg ctgtctcgac ccatatttcc ttatccgcat agtatcaggt
    29461 gcgctcctcc tgcctcacgt atatactttt gctctttcga tatcattcat atcaatatca
    29521 tgcaaaagaa atcaatttac gtcgcctaca cgggcgggac catcgggatg cagcgttccg
    29581 agcagggtta tattccggtg tcaggtcatc tacaacgcca actggcgctg atgccggaat
    29641 tccatcgccc ggagatgcca gatttcacca ttcatgaata tacgccgttg atggattctt
    29701 ctgatatgac gccggaagac tggcagcata ttgctgaaga tattaaagcg cactatgacg
    29761 actatgatgg ttttgtcatt ctacacggta ccgacacgat ggcgtatacc gcctctgcgc
    29821 tgtcgttcat gctcgagaat ctcggtaaac cggtcattgt gacagggtca caaatcccgc
    29881 tggctgagtt acgctctgac ggacaaatta atctgctgaa tgcgttgtac gttgcggcga
    29941 attatccgat caacgaagta acgctctttt tcaataaccg attgtatcgc ggcaaccgca
    30001 ctaccaaagc ccatgccgat ggttttgatg cgtttgcctc tccaaacctt cctccgttac
    30061 tggaagctgg tatccatatt cgtcgtttga atacgccacc cgccccgcac ggtgaagggg
    30121 aattaatcgt tcatccaatc acgccacaac caattggcgt agtgacgatt tatccgggga
    30181 tttctgctga tgtcgtgcgc aattttctgc gccaaccggt gaaagcattg attctgcgct
    30241 cctatggcgt gggtaatgcg ccacaaaaca aagccttcct gcaggaatta caagaagcca
    30301 gcgatcgcgg tattgtggtg gttaacctga cacaatgtat gtccggtaaa gtgaacatgg
    30361 gtggttatgc caccggtaac gccctcgccc atgccggcgt aattggcggt gcagatatga
    30421 ctgtagaagc tacgctaacc aaactgcatt acctgctgag ccaggaactg gatactgaaa
    30481 ccattcgcaa ggccatgagc caaaacctgc gcggcgaact gacgccggat gattaaggag
    30541 actgcaatgc cccctcgcgc cctgttactg gtcgatttac aaaatgattt ctgtgctggt
    30601 ggcgcgctcg ccgtgccgga aggtgacagt acggtggatg tcgctaaccg ccttattgac
    30661 tggtgccagt cgcgcggtga agcggttatc gccagtcagg actggcaccc ggcgaatcac
    30721 ggcagttttg ccagtcagca ctgtgtagag ccttatatgc caggtcaact cgacggtttg
    30781 ccacaaacct tctggccaga tcactgtgtg cagaacagtg aaggcgcaca attacatccg
    30841 ttactgaacc aaaaagcgat cgcagcggtg ttccataaag gcgaaaatcc tttagttgac
    30901 agttacagtg ccttttttga taacggccgt cggcagaaaa cctctctcga tgactggtta
    30961 cgcgatcatg aaatcgatga attgatcgtt atgggcctgg ctactgacta ttgcgtgaag
    31021 tttaccgtgc tggacgcgtt acagttaggt tataaagtaa acgtgattac cgatggttgt
    31081 cgtggcgtga atatccagcc ccaggacagt gcgcacgcgt ttatggagat gtcagcagct
    31141 ggggcaacgc tatatacgct ggcagactgg gaagagacgc aggggtaata ttagttgaat
    31201 tcatctctgg cctacatttc ctcaatgaca ttgtaggcca gataatactt gtcagtgttg
    31261 catttaacct acttgcagct aaaacacgac gtcctaacaa gtatatttgc cctcccttaa
    31321 taacaggctt aaatactaga taactcattc acttaaacat agcaataggc tttgctgaga
    31381 taccaaaatc ttcttttaac tgctgtttgc tcttcatgac catctgacca tttgtgtcaa
    31441 ttgtcatatg ctgtgcttcg gtattatggc gggcttgcca cagcatcacc agttgcaggc
    31501 aattctcttt ttgttcctcg gtcaacgcaa cgccatcagg ccatttcccc agttcaacgg
    31561 cggtcgacaa acgctggtat acctcaggcg tcatgctgtt gataatgtca tcaagattca
    31621 taatttttcc gctcctgtgg aataagttgc tgaatcgttt tttcaaccgt tgatttcttc
    31681 gccgttttcg ccatcagtga agcgtaaaga agcagagtta acacaataac gttcgcctgt
    31741 tggttgcggc ccgtcgggaa agacatgccc cagatgggca tcacagttac cgcaacgaat
    31801 ttctatgcgc tgcattccat gtgacaagtc tttgatataa cgaatggatt cttcactcac
    31861 cggttcgtag aaactgggcc agccacagcc ggaatcatac ttggtttcgg aatgaaacag
    31921 cggggcatcg cagatcaaac agtgatatac gccgtcacgc ttgttatgca gtaaacgacc
    31981 cgtaaatggc ggttctgtcc catgattctg cgtcacgtaa aactgcatct cggacaaatt
    32041 ttttttcagt tcttctgccg aaggtttatt agccatttgc tcacatctca ctttaatcgt
    32101 gctcacatta cgtgactgat tctaacaaaa cattaacacc aactggcaaa attttgtcct
    32161 aaacttgatc tcgacgaaat ggctgcacct aaatcgtgat gaaaatcaca tttttatcgt
    32221 aattgccctt taaaattcgg ggcgccgacc ccatgtggtc tcaagcccaa aggaagagtg
    32281 aggcgagtca gtcgcgtaat gcttaggcac aggattgatt tgtcgcaatg attgacacga
    32341 ttccgcttga cgctgcgtaa ggtttttgta attttacagg caacctttta ttcactaaca
    32401 aatagctggt ggaatatatg actatcaaag taggtatcaa cggttttggc cgtatcggtc
    32461 gcattgtttt ccgtgctgct cagaaacgtt ctgacatcga gatcgttgca atcaacgacc
    32521 tgttagacgc tgattacatg gcatacatgc tgaaatatga ctccactcac ggccgtttcg
    32581 acggtaccgt tgaagtgaaa gacggtcatc tgatcgttaa cggtaaaaaa atccgtgtta
    32641 ccgctgaacg tgatccggct aacctgaaat gggacgaagt tggtgttgac gttgtcgctg
    32701 aagcaactgg tctgttcctg actgacgaaa ctgctcgtaa acacatcact gctggtgcga
    32761 agaaagtggt tatgactggt ccgtctaaag acaacactcc gatgttcgtt aaaggcgcta
    32821 acttcgacaa atatgctggc caggacatcg tttccaacgc ttcctgcacc accaactgcc
    32881 tggctccgct ggctaaagtt atcaacgata acttcggcat catcgaaggt ctgatgacca
    32941 ccgttcacgc tactaccgct actcagaaaa ccgttgatgg cccgtctcac aaagactggc
    33001 gcggcggccg cggcgcttcc cagaacatca tcccgtcctc taccggtgct gctaaagctg
    33061 taggtaaagt actgccagaa ctgaatggca aactgactgg tatggcgttc cgcgttccga
    33121 ccccgaacgt atccgtagtt gacctgaccg ttcgtctgga aaaagctgca acttacgagc
    33181 agatcaaagc tgccgttaaa gctgctgctg aaggcgaaat gaaaggcgtt ctgggctaca
    33241 ccgaagatga cgtagtatct accgatttca acggcgaagt ttgcacttcc gtgttcgatg
    33301 ctaaagctgg tatcgctctg aacgacaact tcgtgaaact ggtatcttgg tacgacaacg
    33361 aaaccggtta ctccaacaaa gttctggacc tgatcgctca catctccaaa taagttgaga
    33421 tgacactgtg atctaaaaag agcgacttcg gtcgctcttt tttttacctg ataaaatgaa
    33481 gttaaaggac tgcgtcatga ttaagaaaat ttttgccctt ccggtcatcg aacaaatctc
    33541 ccctgtcctc tcccgtcgta aactggatga actggacctc attgtggtcg atcatcccca
    33601 ggtaaaagcc tcttttgcat tacagggcgc acaccttctc tcgtggaaac ctgcgggtga
    33661 agaagaagtt ctgtggttga gcaacaacac accgttcaaa aatggcgtcg ctattcgcgg
    33721 tggcgtaccg gtttgctggc cgtggtttgg tccggcggca caacaaggtc tgcctgcgca
    33781 cggttttgcc cgcaacctgc cgtggacgct gaaatcacat cgtgaagatg ctgatggcgt
    33841 aacgctgact tttgaattga cgcaaagcga agagacgaaa aaattctggc cgcacgactt
    33901 tacgctgtta gcgcatttcc gcgtgggtaa aacctgtgaa atcgatcttg aatcacatgg
    33961 cgaatttgaa accacctctg ccctgcatac ctactttaac gtgggtgata tcgctaaggt
    34021 aagcgtcagt gggctgggcg atcgcttcat tgataaagta aatgacgcga aagaagatgt
    34081 actgaccgac ggtattcaga ccttccctga ccgtaccgat cgcgtgtatc tgaatccgca
    34141 agattgcagc gtgattaatg atgaagcgct gaatcgtatt atcgccgtag gccaccagca
    34201 tcatctgaac gttgtcggct ggaacccggg accggcgctt tcagttagca tgggcgacat
    34261 gccagatgat ggctacaaaa catttgtttg tgtagaaacg gcttacgctt cagaaacgca
    34321 aaaagtgacc aaagagaaac ctgcacatct ggcgcaatcc attcgcgttg cgaaacgtta
    34381 atttgcgtta atgttgtgtg ccgggtgcga tgcatccggc acgtatcaac atcacaccat
    34441 atccagcgca gtttttcctt ttggtgccgg atatgcttta tccagcatga ttaattccgc
    34501 tgacgaaagt tcgacctcaa gcacagccgc attctgttgg acatgggcaa tcgtggccgc
    34561 ttttggaatc gccatcacac cctgatggct gattacccac gccaacaata cttgtgccgc
    34621 gctgatattg tgagcatgtg caatttcgtt gactaccgcg ttttttaaca gtccattgcg
    34681 caaccgcccg gcctgggcta atggactgta agccattacc ggcatctgct gttgctggca
    34741 ccaggggagt agatcgtact caatccctcg tgaaccgaga tggtaaagca cctgattagt
    34801 ggcacactga tttccccccg gcagctgcca gagttcctgc atatcagcat aatcaaggtt
    34861 agaaacgccc cagcggcgga tttttccctg ggcgatcaat ttttccatcg ctgccacagt
    34921 ctcttcaaaa gcgaaactgc cagaccagtg taataagtag agatcgagat aatcagtatt
    34981 gagacggcgt aaactggctt cgcatgcatt tatcgctttt tgcccgccag cattccacgg
    35041 atagacttta gagacgagaa agacgttatc gcgcagatcg gttaatgctt ccccaaccac
    35101 cttttcggca ccgccatcgg catacatttc ggcggtatca atgagggtta aaccgagctc
    35161 aatgcccgcg cgtagtgcag caacttctgt tttgcgctga ctggcatctt cgcccatata
    35221 ccatgttccc tgccctatgg ctggcagtga gacatcgcca ctaaattgaa tcattttttg
    35281 ttgcattgtt tcctcccagg tattgcacca ccgtaatgca aaacagggcg tgacgccctg
    35341 tttttatgca caaaatgccc tggaaagatg cattatcaga atttgtaggt gatcccggta
    35401 gaaatcaggc cagtccagga tttatccacc atcgggctgt cagtaacttc atcagacaga
    35461 cgggtatagc gcgcggtacc gtaaacactc cagtcgccga ggaagttgta gctggcgctc
    35521 agctccaggt aagggctcca gctgtcgttc gggttatagc cacgcagacc gctgcgagcg
    35581 gactctttgc gcgatacgcc ataatagtat tcgttctggt tttcgctgtt ccactgcaca
    35641 ccaatacccg gagtcacggt caggccaccg ttggtgtaac gatacaacca ggccatatcc
    35701 cagacaatgc cgttgctgtt atccagggta tcgccagcca aggtggtacg caggtaaccg
    35761 tactgggtaa agtgagcata agacagacca gccatcatgg tgctcttacg gtcatccagg
    35821 tgacgcattt ggtgatcgcc actgtctttc gctttgaagt aaagcggcga ccagtaagcg
    35881 gtaattgaaa gtttatccgt tgcgtcattc cacaggtagt aaccaccacc taagccacgg
    35941 aaccagaagt tatcgccttc atagttgatt accggtactg ggtaaacatc ggtatcgtaa
    36001 tctttatatg ggtgctcaac gacacctacg cctgcgccca gggaaaattt accttcagcg
    36061 tgcgctacgc ctgcagacgt tgcgataagc actccaagtg ccagaagttt gagtttggtc
    36121 acaattaatc attccttaaa caaatgttta gcgggcgaca aagtttaccc gtcaatacat
    36181 cgaaacccaa cctttttacg ttttcatttt ttaaagtaac tgtttaattt tcctgacgcg
    36241 gatgacaccg cgcttacagc caaatgaatt tagcgttact ggctaccctg gtctttacat
    36301 taattatgca aaatttatgg atgagttgtt gatatgccat tgaaattaag aaagccgtgc
    36361 aggcaagttt tccatttgcc atctacgctt aattttgaag gtgtatcacc gggcacgttg
    36421 ttctcatcgt cgataaaatg gcatgagagt tgctgtgttt tagcaagaga cgtcgttcag
    36481 tttacctctt ccgggagcct ctactattca tatgaacggc tcttaacctg tgctaaaaaa
    36541 cgaaaggacg gcataccatg aatatattcg atcactatcg ccagcgatat gaagctgcca
    36601 aggacgaaga gttcacactg caggagtttc ttaccacttg tcggcaagat cgcagtgctt
    36661 atgccaacgc ggctgagcgg ctattgatgg ctatcggtga gcctgtcatg gtcgatacag
    36721 cccaggaacc cagactttct cgactctttt ctaaccgggt cattgcacgt tatccggcat
    36781 ttgaagagtt ttacggcatg gaagacgcga ttgaacagat tgtctcttat ctgaaacacg
    36841 cggctcaggg gctggaagag aagaaacaaa tcctgtatct gctggggcct gtgggtgggg
    36901 gtaaatcatc gcttgctgag cgactgaaat cattaatgca gctcgtaccg atttatgtat
    36961 tgagcgccaa cggtgagcgt agcccggtca acgatcatcc gttctgtctt ttcaatccgc
    37021 aggaagatgc tcagattctg gaaaaagagt atggcattcc tcgccgttat ctcggcacca
    37081 tcatgtcgcc atgggcggca aaacgcctgc atgaatttgg tggcgatatc actaagttcc
    37141 gggtagtgaa ggtctggccg tcaattctgc aacaaattgc tatcgccaaa acggaacccg
    37201 gtgatgagaa caaccaggac atctccgcgc tggttgggaa agtcgatatt cgtaaactcg
    37261 aacactacgc gcagaatgac ccggacgcct acggctattc cggtgcgctg tgccgcgcca
    37321 accaagggat catggaattt gtcgagatgt ttaaagcacc gataaaagtg ctgcatccgc
    37381 tgttaaccgc cactcaggaa ggtaactaca acgggacgga aggtatctcc gccctgccgt
    37441 tcaacgggat tattctcgcc cactcgaacg aatccgaatg ggtcactttc cgtaataaca
    37501 aaaacaacga agccttcctc gaccgtgttt acatcgtgaa ggtgccgtat tgcttacgca
    37561 tttccgaaga gatcaaaatc tacgagaaat tgcttaatca cagtgaattg actcacgccc
    37621 cctgcgcccc tggcacgctg gaaacgctgt cacgtttttc cattctttcg cgccttaaag
    37681 agccagaaaa ctccagcatt tattcaaaga tgcgggttta tgatggcgaa agtctgaaag
    37741 acaccgatcc caaagccaag tcgtatcagg aatatcgtga ctacgccggt gtcgatgaag
    37801 ggatgaacgg tctgtcgacg cgttttgcgt ttaagatcct ctcccgcgtg ttcaacttcg
    37861 atcatgtaga agtggcggca aacccggtcc atctgttcta cgtactggaa cagcagatcg
    37921 agcgcgagca gttcccacaa gagcaggcag aacgctatct ggagttcctg aaaggttatc
    37981 tgatcccgaa atatgccgag tttatcggca aagagatcca gacggcctac cttgaatcct
    38041 attccgaata tgggcaaaac attttcgacc gttatgttac ctacgcggat ttctggattc
    38101 aggatcagga gtatcgcgat ccggataccg ggcagctgtt tgaccgcgag tctcttaacg
    38161 ccgagctgga gaaaatcgag aaaccggcgg ggatcagtaa tccaaaagat ttccgcaacg
    38221 agattgttaa cttcgtactg cgcgccagag cgaataacag cggacgcaat ccgaactgga
    38281 ccagctatga aaaactgcgc acggttatcg agaagaaaat gttctccaat accgaggagc
    38341 tgttgccggt tatctcgttt aacgccaaaa cgtcaaccga cgagcagaag aaacacgacg
    38401 actttgttga ccgtatgatg gaaaaaggct acacccgtaa acaggtgcgt ttactgtgcg
    38461 aatggtattt gcgcgtacgt aaatcgtctt aacaaccctg gcccggtaca catgttaccg
    38521 ggcctacaac gacagcgaac cgtgggcctg agaagcggca acacaggcgt agcatacagt
    38581 tggcaaatgt agtacggggg gcatatgacc tggtttattg accggcgtct gaacggcaaa
    38641 aacaaaagca tggtgaatcg ccagcgtttt ttacgccgtt ataaagcgca aattaaacag
    38701 tcgatctccg aggccattaa taagcgttcg gtgactgacg tcgacagcgg cgaatccgta
    38761 tccattccca cggaagatat tagcgaaccg atgtttcatc aggggcgtgg cggtctgcgc
    38821 caccgcgtgc atccgggcaa tgaccatttc gtccagaacg accgaattga acgtccccag
    38881 ggtggcggcg gaggttccgg cagtggtcag ggccaggcca gccaggatgg tgaaggtcag
    38941 gatgaatttg tctttcagat ttcgaaagat gagtatcttg atttgctctt tgaagatttg
    39001 gccttaccga atctgaaaca aaaccaacaa cgccagctga ccgaatataa aacgcatcgg
    39061 gcgggttata ccgctaacgg cgttccggca aatatcagcg ttgtgcgctc cttgcagaac
    39121 tcactggcgc gacgcacagc catgacggca ggcaagcggc gggaacttca tgcactggaa
    39181 gagaatttgg ccatcatcag caacagtgaa cctgcgcagc tgctggaaga ggaacgtctg
    39241 cgcaaagaaa ttgcagaatt acgtgccaaa attgaacgcg tcccttttat tgacaccttc
    39301 gatttacgtt acaagaacta cgagaagcgg cccgatccct ccagccaggc agtgatgttt
    39361 tgcctgatgg acgtttccgg ttcaatggat caatccacta aagatatggc taagcgtttt
    39421 tatattctgc tgtatctgtt cctcagcaga acgtataaga acgtggaagt cgtatacatc
    39481 cgccatcata cccaggcgaa agaagtcgat gaacatgagt ttttctactc gcaggaaaca
    39541 ggcggcacca tcgtttccag cgccctgaaa ctgatggatg aggtagtgaa agagcgttat
    39601 aacccggcac agtggaatat ttacgctgca caagcatcgg acggcgataa ctgggccgat
    39661 gactctccgc tttgccatga aatcctggcg aaaaaattat tacctgttgt tcgttattac
    39721 agctatatcg aaattacccg tcgtgcacat cagacattgt ggcgagaata tgagcatctg
    39781 caatctactt tcgacaactt tgcgatgcag cacatccgcg accaggatga tatttatccg
    39841 gtgttccgtg aactgtttca taaacaaaat gcaacagcta aagactaaaa ctatcagcca
    39901 ggtcattatc gcctggctga tttttagctt actgtaaatt atctcattta ttacatacat
    39961 tagcttacaa tcgctttaaa tatgacagca taacctttac ataatttagt tccagaaaac
    40021 aatcattcgg aaaaatgatt cagtcaacac gtatttccat ggggttattc tttaaatatt
    40081 ttttatcgtt aa
//
LOCUS       DAZGVQ010000026        38668 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000026 DAZGVQ010000000
VERSION     DAZGVQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.026"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            48..467
                     /gene="umuD"
                     /locus_tag="ACHLAG_001332"
     CDS             48..467
                     /gene="umuD"
                     /locus_tag="ACHLAG_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFP0654592.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            467..1735
                     /gene="umuC"
                     /locus_tag="ACHLAG_001333"
     CDS             467..1735
                     /gene="umuC"
                     /locus_tag="ACHLAG_001333"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFP0654593.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1781..2311)
                     /gene="dsbB"
                     /locus_tag="ACHLAG_001334"
     CDS             complement(1781..2311)
                     /gene="dsbB"
                     /locus_tag="ACHLAG_001334"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFP0654594.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2457..3998)
                     /gene="nhaB"
                     /locus_tag="ACHLAG_001335"
     CDS             complement(2457..3998)
                     /gene="nhaB"
                     /locus_tag="ACHLAG_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFP0654595.1"
                     /translation="MEISWGRALWRNFLYQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHIREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4219..4938
                     /gene="fadR"
                     /locus_tag="ACHLAG_001336"
     CDS             4219..4938
                     /gene="fadR"
                     /locus_tag="ACHLAG_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFP0654596.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4990..6522)
                     /gene="ycgB"
                     /locus_tag="ACHLAG_001337"
     CDS             complement(4990..6522)
                     /gene="ycgB"
                     /locus_tag="ACHLAG_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFP0654597.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            6852..8150
                     /gene="dadA"
                     /locus_tag="ACHLAG_001338"
     CDS             6852..8150
                     /gene="dadA"
                     /locus_tag="ACHLAG_001338"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFP0654598.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQSTFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8160..9230
                     /gene="dadX"
                     /locus_tag="ACHLAG_001339"
     CDS             8160..9230
                     /gene="dadX"
                     /locus_tag="ACHLAG_001339"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFP0654599.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGVTDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9616..11352)
                     /gene="cvrA"
                     /locus_tag="ACHLAG_001340"
     CDS             complement(9616..11352)
                     /gene="cvrA"
                     /locus_tag="ACHLAG_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFP0654600.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11447..12361)
                     /gene="ldcA"
                     /locus_tag="ACHLAG_001341"
     CDS             complement(11447..12361)
                     /gene="ldcA"
                     /locus_tag="ACHLAG_001341"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFP0654601.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12461..13072
                     /gene="emtA"
                     /locus_tag="ACHLAG_001342"
     CDS             12461..13072
                     /gene="emtA"
                     /locus_tag="ACHLAG_001342"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFP0654602.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13074..13808)
                     /gene="ycgR"
                     /locus_tag="ACHLAG_001343"
     CDS             complement(13074..13808)
                     /gene="ycgR"
                     /locus_tag="ACHLAG_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFP0654603.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMRQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14009..14263
                     /gene="ymgE"
                     /locus_tag="ACHLAG_001344"
     CDS             14009..14263
                     /gene="ymgE"
                     /locus_tag="ACHLAG_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP0654604.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            14441..14881
                     /gene="ycgY"
                     /locus_tag="ACHLAG_001345"
     CDS             14441..14881
                     /gene="ycgY"
                     /locus_tag="ACHLAG_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFP0654605.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRYKSPGDDSETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(14960..16657)
                     /gene="treA"
                     /locus_tag="ACHLAG_001346"
     CDS             complement(14960..16657)
                     /gene="treA"
                     /locus_tag="ACHLAG_001346"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFP0654606.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFAMMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(16977..18395)
                     /gene="dhaM"
                     /locus_tag="ACHLAG_001347"
     CDS             complement(16977..18395)
                     /gene="dhaM"
                     /locus_tag="ACHLAG_001347"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFP0654607.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKSICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKRQRFNRQG"
     gene            complement(18406..19038)
                     /gene="dhaL"
                     /locus_tag="ACHLAG_001348"
     CDS             complement(18406..19038)
                     /gene="dhaL"
                     /locus_tag="ACHLAG_001348"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFP0654608.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19049..20119)
                     /gene="dhaK"
                     /locus_tag="ACHLAG_001349"
     CDS             complement(19049..20119)
                     /gene="dhaK"
                     /locus_tag="ACHLAG_001349"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFP0654609.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLMI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLVNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRFQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20347..22266
                     /gene="dhaR"
                     /locus_tag="ACHLAG_001350"
     CDS             20347..22266
                     /gene="dhaR"
                     /locus_tag="ACHLAG_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFP0654610.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPVDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22366..25233)
                     /gene="ycgV"
                     /locus_tag="ACHLAG_001351"
     CDS             complement(22366..25233)
                     /gene="ycgV"
                     /locus_tag="ACHLAG_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0654611.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAHNGFYSDLVVKASRQKNSFHVLDSQNNG
                     VKANGTANGLSISLEAGQRFNLTPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNMYVKTGAIREFSGDTDYLLNNSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQSNLFDQKQVNGGYRFSF"
     gene            complement(26074..27165)
                     /gene="ychF"
                     /locus_tag="ACHLAG_001352"
     CDS             complement(26074..27165)
                     /gene="ychF"
                     /locus_tag="ACHLAG_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFP0654612.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(27282..27866)
                     /gene="pth"
                     /locus_tag="ACHLAG_001353"
     CDS             complement(27282..27866)
                     /gene="pth"
                     /locus_tag="ACHLAG_001353"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFP0654613.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            28144..28422
                     /gene="ychH"
                     /locus_tag="ACHLAG_001354"
     CDS             28144..28422
                     /gene="ychH"
                     /locus_tag="ACHLAG_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFP0654614.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(28477..30156)
                     /gene="dauA"
                     /locus_tag="ACHLAG_001355"
     CDS             complement(28477..30156)
                     /gene="dauA"
                     /locus_tag="ACHLAG_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFP0654615.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(30281..31228)
                     /gene="prs"
                     /locus_tag="ACHLAG_001356"
     CDS             complement(30281..31228)
                     /gene="prs"
                     /locus_tag="ACHLAG_001356"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP0654616.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(31379..32230)
                     /gene="ispE"
                     /locus_tag="ACHLAG_001357"
     CDS             complement(31379..32230)
                     /gene="ispE"
                     /locus_tag="ACHLAG_001357"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFP0654617.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLIKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(32230..32853)
                     /gene="lolB"
                     /locus_tag="ACHLAG_001358"
     CDS             complement(32230..32853)
                     /gene="lolB"
                     /locus_tag="ACHLAG_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFP0654618.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            33067..34323
                     /gene="hemA"
                     /locus_tag="ACHLAG_001359"
     CDS             33067..34323
                     /gene="hemA"
                     /locus_tag="ACHLAG_001359"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFP0654619.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            34365..35447
                     /gene="prfA"
                     /locus_tag="ACHLAG_001360"
     CDS             34365..35447
                     /gene="prfA"
                     /locus_tag="ACHLAG_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFP0654620.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYSLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            35447..36280
                     /gene="prmC"
                     /locus_tag="ACHLAG_001361"
     CDS             35447..36280
                     /gene="prmC"
                     /locus_tag="ACHLAG_001361"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP0654621.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            36277..36669
                     /gene="sirB2"
                     /locus_tag="ACHLAG_001362"
     CDS             36277..36669
                     /gene="sirB2"
                     /locus_tag="ACHLAG_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFP0654622.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            36673..37482
                     /gene="sirB1"
                     /locus_tag="ACHLAG_001363"
     CDS             36673..37482
                     /gene="sirB1"
                     /locus_tag="ACHLAG_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFP0654623.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            37518..38372
                     /gene="kdsA"
                     /locus_tag="ACHLAG_001364"
     CDS             37518..38372
                     /gene="kdsA"
                     /locus_tag="ACHLAG_001364"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFP0654624.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSMMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(38521..38628)
                     /gene="ldrA"
                     /locus_tag="ACHLAG_001365"
     CDS             complement(38521..38628)
                     /gene="ldrA"
                     /locus_tag="ACHLAG_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0654625.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         9593 a         9653 c         9956 g         9466 t
ORIGIN      
        1 gaacagacta ctgtatataa aaacagtata acttcaggca gattattatg ttgtttatca
       61 agcctgcgga tctccgcgaa attgtgactt ttccgctatt tagcgatctt gttcagtgtg
      121 gctttccttc accggcagca gattacgttg aacagcgcat cgatctgaat caactgttga
      181 tccagcatcc cagcgcgact tacttcgtca aagcaagtgg tgattctatg attgatggtg
      241 gaattagtga cggtgattta ctgattgtcg atagcgctat taccgccagc catggtgata
      301 ttgtcatcgc tgctgttgac ggcgagttta cggtgaaaaa attgcaacta cgcccgacgg
      361 tacagcttat tcccatgaac agtgcgtact cgcccattac catcagtagc gaagatacgc
      421 tggatgtctt tggtgtggtg atccacgtcg ttaaggcgat gcgctgatgt ttgctctctg
      481 tgatgtaaac gcgttttatg ccagctgtga gacggtgttt cgccctgatt tatggggtaa
      541 accggtggtt gtgctatcga ataatgacgg ttgcgttatc gcccgaaacg ctgaggcaaa
      601 ggcgctgggt gttaaaatgg gcgatccctg gttcaaacaa aaagatctgt ttcgtcgctg
      661 tggcgtggtt tgctttagca gcaattatga gctttacgca gacatgagca atcgggtgat
      721 gtcgacgctg gaagaactat cgccccgcgt cgagatttac agtattgatg aggcattctg
      781 cgatctgaca ggtgtgcgta attgtcgcga tctgactgat tttggcagag aaattcgcgc
      841 aacggtgcta caacgtaccc atcttactgt tggtgtgggg atcgcccaga ccaaaacgtt
      901 ggctaagctt gccaatcatg cggcaaaaaa atggcagcgg cagacgggtg gggtggtgga
      961 tttatcaaat ctggaacgcc agcgtaaatt aatgtctgct ctccccgtgg atgacgtctg
     1021 ggggattgga cggcggatca gcaaaaaact ggacgcgatg gggatcaaaa ccgttctcga
     1081 tttggcggat acagatatcc ggtttatccg taaacatttt aatgtcgtgc tcgaaagaac
     1141 ggtgcgtgaa ctgcgcggcg aaccctgttt gcaactggaa gagtttgcac cgacgaagca
     1201 ggaaattatc tgttcccgct cgtttggtga acgcatcacg gattatccat cgatgcggca
     1261 ggccatttgt agttacgctg cccgggcggc ggaaaaactt cgcagcgagc atcaatattg
     1321 tcggtttatc tccacattta ttaagacgtc accatttgcg ctcaatgaac cttattacgg
     1381 caatagcgcg tcggtaaaac tgctgacgcc cactcaggac agcagggata tcattaacgc
     1441 tgctacgcga tctctggatg ccatctggca agcgggccat cgctaccaaa aagcgggcgt
     1501 gatgctgggg gatttcttca gtcagggagt cgcgcagctc aatttattcg atgacaacgc
     1561 accgcgcccc gggagtgagc aattgatgac ggtaatggat acactgaatg ctaaagaggg
     1621 cagaggaaca ctctattttg ccgggcaggg gatccagcaa caatggcaga tgaagcgtgc
     1681 catgctttca ccacgttata caacgcgaag ttctgattta ctgcgggtca aataaatata
     1741 gcggcaggaa aaaagcgctc ccgcaggagc gctgaaggga ttagcgaccg aacaggtcac
     1801 gttttttcgc tttaaacggc tgggaaatca ccaccagcac cgcgacaatc aggtaagcga
     1861 taaaaatacc gagcagccac tgcggcattt ccatacctaa aaaatcccac tgacgctcgg
     1921 cacaatcgcc agaggcgaca aacacttgcg gcacccattt atccagcggc agccattccg
     1981 ggaaacgaac cataaaatca caggtggcaa acggcgaagg atagagctga agcatggtgt
     2041 gctcgtaagt taactgcaca ccgcggaacg cactatacaa ccagataacc atcgctacat
     2101 aacgcagcgg agttttcggg gcgatcgcgc caatcagcgc agcacccaga acgccgaata
     2161 acgcacaacg ttcataaata cagagcacgc aaggtttcag taacatcaca tgctggaacc
     2221 atagcgccgt cagttccagt gccagagcag taaacgccat caacagccac gcgccccggc
     2281 cttgtgaaca ttggttcaaa aatcgcaaca taatcatttc cctgcaatat gcatagagtg
     2341 cgcagtttaa accaattcat tcgctgcgcc accaggaggc agcaaaaata ggaagcaatt
     2401 ggacaattat cgggcgaaaa ggcaaaccgg gcatttatat gcccggtaag ttgttatcaa
     2461 agcgttgcta tccagcccat ttgcataaac cattcggtta caggggcaag cgtaaactcg
     2521 acgcagagca agccgacgag tgtcagtacg agggtgtaag gcagggccat ccacaccatg
     2581 cggccataag agaggcgaat caatggcgcg agtgcagagg tcagcaggaa caggaacgca
     2641 gcctgaccgt tcggcgtagc gacggagggc agattggtac cggtattaat ggcgaccgcc
     2701 agcagctcgt attgcttcaa cgtgatagcg ccactttcca ttgccgcttt cgcttcgttg
     2761 atataaatcg tccccacgaa gacgttatcc gaaatggatg acagcagacc gttgaaaata
     2821 tagaacagcg acagctgagc atgttccgat gcctgcaaca caaactgaat aattggcgaa
     2881 aacagttgtt ggtcgataat caccgcgact accgagaaaa acaccgtcaa cagtgcggtg
     2941 aatggcagag attcggtgaa ggctttaccg atagcatgtt catcggtgac accggtcaat
     3001 gatgtcgcca gaataatgac tgacaaacca atcaagccaa cttccgccag atgcaacgcc
     3061 agcgcagtca ccagccagac gccaataatc gcctggacaa tcagacggat tttatcctga
     3121 cgggtacgct ggtggcggct ttgatcgtca aactgttgca acacttcgcg gactttctcc
     3181 ggcagcgttt caccgtagcc aaaccaacgc agcttctcta ccagcaggca ggttaacagg
     3241 ccacaaatca gaaccggaac ggtcaccggc gacatgcgca ggaagaaatc gccaaaatgc
     3301 cagccagccg ctttagcgat gatcaggttc tgtggttcgc ccaccatggt catcacgccg
     3361 cctaatgcag tgccgacacc ggcatgcatc atcaggctac gcagaaagcc acggaactgt
     3421 tccagaacca ctttgtaatg cttgtcgata tgactatcgt cttgcaggtc ggtatcttca
     3481 gtacgggaag aggctacgcg gtgataaata ccataaaaac cgactgcaac gctgatcacc
     3541 accgccacga cggttaaggc atcgaggaac gcggagagga acgccgccgc cacgcaaaaa
     3601 gagagcgaca gcagcatttt ggagcgaatg cttaacagca aacgggtaaa tatgaacagc
     3661 aacagctgtt tcataaaata gatacccgcc accataaaca tcaataacag caagacttca
     3721 agatttgccg ccacctcttc acggatgtgt tccgcgctgg tcatgccgat gaataccgct
     3781 tcgatagcca acagaccacc ggggagtagc gggtagcatt tcagggccat cgccagagtg
     3841 aaaataaatt ccgctaccag caaccagccc gcgacgaaag ggctgatgag gaaaattaac
     3901 gggtttacga ttaagaaaat gatgagggcg agtttgtacc agtcggggga ctggtacaaa
     3961 aaattgcgcc atagcgcgcg gccccaggag atctccatga tggtttccct taccttacaa
     4021 ataatcaatg atgtttttat gtttaaacgc aaagcttaac ggtcaggcag gagtgaggca
     4081 agtcttgata gtcaagggga aagagatgcg gaaaatgaag ccttgatccc tttttcttct
     4141 ttttgtctgc tatcagcgta gttagccctc tggtatgatg agtccaactt tgttttgctg
     4201 tgttatggaa atctcactat ggtcattaag gcgcaaagcc cggcgggttt cgcggaagag
     4261 tacattattg aaagtatctg gaataaccgc ttccctcccg ggactatttt gcccgcagaa
     4321 cgtgaacttt cagaattaat tggcgtaacg cgtactacgt tacgtgaagt gttacagcgt
     4381 ctggcacgag atggctggtt gaccattcaa catggcaagc cgacgaaggt gaataatttc
     4441 tgggaaacct ctggtttaaa tatccttgaa acactggcgc gactggatca cgaaagtgtg
     4501 ccgcagctta ttgataattt gctgtcggtg cgtaccaata tttccactat ttttattcgc
     4561 accgcgtttc gtcagcatcc cgataaagcg caggaagtgc tggctaccgc taatgaagtg
     4621 gccgatcacg ccgatgcctt tgccgagctg gattacaaca tattccgcgg cctggcgttt
     4681 gcttccggca acccgattta cggtctgatc cttaacggga tgaaagggct gtatacgcgc
     4741 attggtcgtc actatttcgc caatccggaa gcgcgcagtc tggcgctggg cttctaccac
     4801 aaactgtcgg cgttgtgcag tgaaggcgcg cacgatcagg tgtacgaaac agtgcgtcgc
     4861 tatgggcatg agagtggcga gatttggcac cggatgcaga aaaatctgcc gggtgattta
     4921 gccattcagg ggcgataatc ccttccgttt aaagagcaaa cccctcaaac gaggggtttt
     4981 ttgttgtttt tacagatttc ccattcttgg cgggcaacgt tccagcaact cgatgctgcc
     5041 gtcttcgttt tgctgttcga gcatcacatc aaatccccac aggcgatgca catgcttcag
     5101 cacttctttg cgcccccgat ccagcggtgc gcgattatgt ggaatgtaac gcagcgtcag
     5161 cgaacggtcg ccgcgcaaat ccacgttcca gatctgaata ttcggctcca gattacttaa
     5221 gttatattgc gacgataacc ggttacggat ctcccgataa ccttcttcat tatgaatagc
     5281 ggaaatctcc agataattat gccgatcatc atccagcacg gtgaagaagc ggaaatcacg
     5341 catcactttc ggtgacagaa actggctgat aaaactctca tctttgaaat cacgcatcgc
     5401 gaaatgcagc gtttccagcc agtcggaacc ggcgatatcc gggaaccagt atttgtcttc
     5461 ttccgttggc gactgacaaa tccgtttaat atcctggaac atggcgaacc cgagggcata
     5521 cgggttgatg ccgctgtacc acgggctgtt ataggggggc tggaagacca cattggtgtg
     5581 gctgtgcaaa aactccagca taaaacgttc cgttactttc ccttcatcat acagatggtt
     5641 aaggatggta tagtgccaga aggtcgccca gccttcgttc atcacctgag tctgtttttg
     5701 cggataaaaa tactggctca ccttacgcac aatacgcagg atttcacgct gccatgattc
     5761 cagcagtggc gcatttttct ccataaaata gagcaggttt tcttgtggtt cggacggata
     5821 gcggcgcgct tcagcaaccg ttttctcttc ctcgcgcttc ggcagggtac gccagagcat
     5881 attgacctga ctttgtagat actcttcgcg acttttctgc cgggctttct cttcttgcag
     5941 cgagattttt tgcgggcgtt tgtaccggtc cacgccgtag ttcatcagcg cgtggcacga
     6001 gtccagaagc cgttctactt catcaacgcc ataacgttct tcgcactcgg taatatattt
     6061 acgggcaaaa atcagataat cgacaatcga actggcgtcg gtccaactgc ggaataagta
     6121 attgttttta aagaaagagt tatgcccata gcaggcgtgt gccatcacca gcgcctgcat
     6181 ggtaatggtg ttctcttcca tcaggtaagc gatacacggg ttagagttaa tgacgatttc
     6241 ataggccagt ccttgctgac cgtgcttata cagccgttca gtctcgataa actttttacc
     6301 gaatgaccag tgcggatagt taattggcat gccgacgctg gagtaggcat ccatcatctg
     6361 ttctgaggtt atcacttcaa tctggtgcgg gtaggtatcc agccggtaga gtttcgccac
     6421 ccggtctatc tctgccagat aaacatccag caggtcgaac gtccagtcgg gtccatcgct
     6481 caaacgtgtg gtgtccttat tcatagaatc gatcgtcgcc atacgcgcac ctcattgttg
     6541 tcggcgctct ctgtgtggag cacctcattt caagcataga acacctgtta aaaaccgcgt
     6601 cgccggagaa tttttttctt tgcgatttct tattatcaga gtgccactaa tccgcttctg
     6661 aacggaattt tatgctggat aaaaagggcg ttcagcagga gatactaaag acgccatatt
     6721 gccgcagagt cagggagatg tgagccagct caccataaaa aagccgtatg ttgaataata
     6781 ttttcaactg agttatcaag atgtaattag attattattc ttttactgta tctaccgtta
     6841 tcggagtggc tatgcgagtt gtcatactgg gaagtggtgt ggtaggcgtt gccagcgcct
     6901 ggtacttaaa tcaggcagga catgaggtca ccgtcattga tcgggagccg ggggccgccc
     6961 tggaaaccag cgccgcgaat gccgggcaaa tctcccccgg atatgctgca ccgtgggcgg
     7021 caccaggtgt gcctttaaaa gcgattaaat ggatgttcca gcgccatgcg ccgctggcgg
     7081 ttcgtctcga cggtacgcag ttccagttga aatggatgtg gcaaatgtta cgtaactgcg
     7141 acaccagcca ctacatggaa aacaaagggc ggatggtgcg tctggcggaa tacagccgtg
     7201 attgcctgaa agcattgcgc gccgaaacca atattcagta tgaagggcgt cagggtggga
     7261 cgctgcaact gttccgtacc gaacaacagt atgaaaatgc gacccgcgat atcgccgtgc
     7321 tggaagatgc cggcgtaccg tatcagctgc tggaatccag ccgcctggcg gaagtggagc
     7381 ccgcgctggc agaagtagcg cacaaactga cgggtggtct gcaactgccc aatgatgaaa
     7441 ccggagactg ccagctgttt acccagaatc tggcgcggat ggcagagcag gcgggggtta
     7501 aattccgctt taatacgccc gttgaccaac tgctttgcga cggcgagcaa atctacggcg
     7561 tgaagtgtgg cgatgaagtg attaaggccg atgcgtatgt gatggcgttt ggttcttact
     7621 cgacggcgat gctcaaaggc attgttgata ttccggtgta tccgctgaaa ggctactcgc
     7681 tgactattcc aattgcgcag gaagatggtg cgccagtatc caccattctt gatgaaacct
     7741 acaaaatcgc cattacccgt ttcgataacc gcattcgtgt tggcggaatg gcggaaattg
     7801 ttggttttaa caccgagctg ttgcaaccgc gtcgtgaaac gctggaaatg gtggttcgcg
     7861 atctctatcc acgcggcggt catgtcgagc agtcgacttt ctggacgggt ttgcgcccga
     7921 tgacgccaga cggtacgccg gttgtcgggc gtacacgctt taaaaatctg tggctgaata
     7981 ccggtcacgg cacgctcggc tggacgatgg cttgcggttc cggtcagttg ttaagcgatc
     8041 tgctctctgg tcgcacgcca gcgatcccat acgaggatct aagcgtagcg cgctacagcc
     8101 gtggatttac gccatcacgt ccgggccatt tacatggcgc acacagctaa ggaaacgaga
     8161 tgacccgtcc gatacaggcc agcctcgatc tgcaggcatt aaaacagaat ctgtccattg
     8221 tccgccaggc cgcgccgcac gcgcgcgttt ggtcggtggt aaaagcgaac gcttacgggc
     8281 atggtattga gcgtatctgg agcgcgctcg gggtcaccga tggctttgca ttacttaacc
     8341 tggaagaggc aataacgtta cgtgagcgcg gctggaaggg gccgatcctg atgctggaag
     8401 gatttttcca tgctcaggat ctggagattt atgaccagca ccgcctgacc acctgcgtac
     8461 acagcaactg gcagctcaaa gcactgcaaa atgcgcggct aaaagcaccg ttggatattt
     8521 atcttaaagt gaacagtggg atgaatcggt tgggcttcca gcccgatcgc gtgcttaccg
     8581 tctggcagca gttgcgggca atggcgaatg ttggcgagat gaccctgatg tcgcattttg
     8641 ccgaagcgga acatcctgat ggaatttcca gcgcgatggc gcgtattgag caggcggcgg
     8701 aagggctgga gtgtcggcgt tcgttgtcca attcggcggc gactctgtgg cacccggaag
     8761 cgcattttga ctgggttcgg cctggcatta ttttgtatgg cgcttcgccg tccggtcagt
     8821 ggcgtgatat cgccaatacc ggattacgtc cggtgatgac gctaagcagt gagattattg
     8881 gtgtccagac gctaaaagcg ggtgagcgtg tgggctacgg cggtcgctat actgcgcgcg
     8941 atgaacagcg aatcggcatt gtcgccgcag ggtacgccga cggttatccg cgccacgcgc
     9001 ctaccggtac ccctgtttta gtggacggcg tgcgcaccat gacggtgggg accgtctcga
     9061 tggatatgct agcggtcgat ttaacgcctt gcccgcaggc ggggattggt acgccggttg
     9121 agctgtgggg caaggagatc aaaattgatg atgtcgccgc cgctgccgga acggtgggct
     9181 atgagttgat gtgcgcgctg gcgttacgcg tcccggttgt gacagtgtaa cttgttgtaa
     9241 gccggatcgg agacagcgtc ttctggttgc gaaaaaatca tccatccggc tggtcagcaa
     9301 ctgtagttgt taatgtgaca gagccattgc ccatgatagt gtccattaaa agaatggaca
     9361 ctatttcccc ggaacctgaa ctcaccgcac aggcgttcta cataaaacgc ttacgcttca
     9421 ttgttgactc gaactcgact tcagataaat cacgctttac ccttgatgga gcctgtacat
     9481 agatttgtgt aattgcctga ttttgatatg ttcaattcaa catcaaatga aggttaaatt
     9541 atggacgaca aacaattgca ggctcaggct gcgttcagca aagcatcgca accggcgata
     9601 gatgcttcat taaatttaag attcagcttc ctcttcagcc acccgtacgc caatcttcaa
     9661 cacttcatta tcttctttct cggccaccgt ccagatcatc ccggcaaact ctacctggtc
     9721 accgacaacc ggtgccgcgc ctaacaactg ctggacaatt tcacccagcg tttgctgctt
     9781 atcacgatat tctcgcccgt cttccaggcc atatatcagc gcaacatcag catatttggc
     9841 gctggcttcg agaatgaagt caccaaagaa gcgttgatcc agcgcgaccg gcggcgactg
     9901 gctgaacagt ttaccgagcg ccggaagatc gcgttcccga ccaattacac acaacacatc
     9961 gccttcgcgc agtcgggtgc tgccggtagg atgaagcaac tggttatcac gaaacagtgc
    10021 cgcaatacgc gtctcttttg gcatatgcaa atcgcgcagt gccgcgccca cgcaccattt
    10081 atcggcactc aattgataaa caaactgctc ccacggattt tccggatgaa tatccaggcc
    10141 aacgcgtgac accggacgtc ccactggcgg aaccaccact ttggcttttt tagccgccca
    10201 cgagagtgat gttccctgca agagcagtga aaccagaacc acaaagaagg cgacattaaa
    10261 gaacagacgt gcattctcca gccccgccat catcgggaac acggccagga tgattggcac
    10321 cgcgccgcgt aatcctaccc agctgataaa cacgcgctca cgcagattga aaccgcggaa
    10381 ggggagcaat ccagcaaata ccgaaagggg acgggcgaag aatatcatcc atgccgacaa
    10441 aatgagcgcc ggaatggcaa taggcagcag atcgcttggg ttaaccaata gccccagcac
    10501 caggaacatg gcgatttgcg ccagccaggc gaggccgtcg aaattttgca ggatgccgta
    10561 gcggttgcga atcggccgat tacccagcag aaaaccgcac agataaacag ccagaatacc
    10621 gctgccttcc agcgccgtgg ttaaagcaaa aattaaaata ccgccgctta atgccagcaa
    10681 tggatataat ccggcaggca gggcgatgcg attaatcatt tgcagcagta aataaccacc
    10741 gccaagtccg atgacaatcc cgaggccaaa ttgctgcaga atatcgacca cgaacatcca
    10801 actgacactg ctttcatgtt gctggatcat cgcaatcagg gtaatcgtca gaaagaccgc
    10861 cattggatca ttactgccgg attcaatttc cagcgtcgag ccaacacgtt cgttaagccc
    10921 cttaccaccc agcagagaaa agaccgctgc agcatcggta gagccgacga tagcgccgat
    10981 taataagcct tcaatcaaat caagattaaa cagccacgcc gccatcatgc cggttaaacc
    11041 agaggtgata agcacgccca gcgtcgccag cgacagtgcc ggtcctaacg ccacacgaaa
    11101 ggaactggcc tgagtgcgca tcccgccgtc gagcaaaata atcgccagtg ccaggttact
    11161 taccatgtag gcgaaggggt aattatcaaa cgggataccg ccgacgccat cgactcctgc
    11221 cagcatgccg atcgccagaa aaataaccag aataggaatg ccaagacggg aagaaaatga
    11281 actaagtaat atactgctgg tgacaaggat ggatcctaag atgaaaaggc taattattgt
    11341 tgtggcatcc aacggtcggt tactcctgat tacgctgtct cttatataaa ccctaccata
    11401 ttagcggcag agacagcgtt ttacttagcc ctgaagcgtg atttttttac attttaagaa
    11461 caggatgacc gctaatcgtc aactgagtgc cttcccgggt gttattcaga atggcatgtg
    11521 cgcccaacgg cagcgtaacc gtgcgttgtt catgaccaaa atcgaggccg gtaatgagtg
    11581 gaatcgacag gcgggaacgc aaaaacgcgt acactgactc caggttgtaa cctgcgtcat
    11641 aatcattggg cgtgctgccg ctaaagctac cgagaataat cgccttctga cgtggcaaaa
    11701 ttcccgcatg atagagctgc aacagcatac gttcgacgcg gaaagggtgc tcgttaatat
    11761 cttccagcac cagaataccg ttctcaattt ttggcatcca cggtgtacca atcagtgaaa
    11821 tcagcatcgc aagattgcct ccccacaacg tgccttcggt ctgacatgtc gggccttccc
    11881 cttgccattc aatggtgaag gtttcattgc gtaacgccag ccagaaatgg tgctcggtaa
    11941 aggcgttcag ttcatccgcg ccaaagtttg ctaccagcat ggggccgcta aaggtgatga
    12001 cattgccttg cgccagaaga ccgcactgaa tggcggtaaa atcgctatgc ccgcatatga
    12061 gcaacgggtc atattgttgg cgagcaacca gtgcctgcca gtcaatatct gccagtaaac
    12121 gactggcacc gtaaccgccg cgtacagcca gcacgatggt gttgggggtg gtcagtctgg
    12181 caagggaatt gagatcttcc aggcgctccg tttccgtacc cgcaaaacgc tcacaacgac
    12241 gggcaataac ctcgacgtta ttgacctgat gcccctcgtc ggtaaggcgc tggataccac
    12301 gcagcgcggc gtgctgttta atgcagtaac ccgatggggc aattaagtga aacagagaca
    12361 tggcaattcc ttgctgacaa cagaaacgaa atgtatatca tgccgcttag gtgtgccgtt
    12421 gtcacctcaa cggcgattcc aggctataag gatagaagaa gtgaaattga gatggtttgc
    12481 ctttttgatt gtgttattag cgggttgttc atcaaagcat gactatacga acccgccgtg
    12541 gaacgcgaaa gttccggtgc aacgtgcgat gcagtggatg ccaataagcc agaaagccgg
    12601 tgcagcctgg ggcgtcgatc cacaattgat cacggcgatt atcgctatcg aatcgggtgg
    12661 taatccgaat gcggtgagta aatcgaatgc cattggtttg atgcagttaa aagcttcaac
    12721 ctccggacgt gatgtttatc gccgtatggg ctggagtggt gagccgacga ccagcgagct
    12781 gaaaaatcca gagcgtaata tttcaatggg ggcggcttac ctgaatattc tggaaaccgg
    12841 cccgctggca ggcattgaag atccgaaggt actgcaatat gcgctggtgg tgtcatacgc
    12901 taacggggca ggtgcgctgc tacggacttt ctcgtcagat cggaaaaagg cgatcagcaa
    12961 aatcaacgat ttggatgctg acgagttcct cgatcacgta gcgcgaaatc accctgcgcc
    13021 gcaggctccg cgctatatct acaaacttga gcaggcactg gacgcgatgt aaatcagtcg
    13081 cgcactttgt ccgctttttc ccgggcttct cgctcgagag agaaaataat ccgctgtaat
    13141 tgccgctcca ccgtcgggct gacgttaaga aaacggaagc tcagacgggg agtggtgatg
    13201 gtttcattct tgccatcaat cactttgcgc tcgctgatgg agattaactg ggcgtcaaag
    13261 tgaaaaacac cccattgcct catgttgact tcaatctgag cgaagcgcat gccttcttgt
    13321 aattcggcag gctttgctgt ttccagtaat gcgcccatgc cgcctaacga caaatcatac
    13381 aggcggaaac gtaacgtact gttatccgcc agtttggtct ggcaaaaata aggcggatgg
    13441 agtggggcgg agatgcggaa atatcggcgt cgttgtacaa accataaggt gggaggcggt
    13501 acggtgataa atgccggaag ctgcaagtat tcactctgct gtagttgttc aacagtaaac
    13561 tcgactttcg caccctgagt ttcggcggta atggtaatgt gctgtgcctt tagcacggcg
    13621 atgttgtctt cggcttgact gccgaaatcc agcaccagtt tatccggggt tattgccagt
    13681 attttgctga tcagctgccc gccattccaa ctgagacgca aaggaattgc ggctttgtgc
    13741 aaatcgcgta acacgcccag gacggctaac ggattttgtt tcaggaactg ctcatggtaa
    13801 tgactcacgc ggaaaaactc ctgactgata aactgtcttt ggtttatcgg tcacagttaa
    13861 caaaacttaa tacaaatgcg tgaatatttt ttacatgttg ttcttaaatc agccgacata
    13921 cgcccagcat cgatcccgcc cttgcctata cttagagcgt tgacgtaagc atttcttgcg
    13981 tccgattcat cgaaacgagg gcctgaacat gggaattatt gcctggatta tttttggcct
    14041 gatagccggc attatcgcca agctaatcat gccggggcgt gatggtggtg gatttttcct
    14101 gacctgtatt ctcgggatag tcggtgcggt ggtcggcggc tggctggcga ccatgtttgg
    14161 cattggcggc tccatcagtg gttttaatct gcacagcttc ctggtggcgg tggtgggagc
    14221 tattctcgtt ctgggcatat tccgcctcct gcgaagagaa taagattttc ataaggcgga
    14281 tagcgataca gatgccgcta tccgctttca catcagaacg tatactcgac acctgcttta
    14341 cgggtgaaaa aaatcaatat agcacttcga tatccgataa agtcggttcc cggtaacaac
    14401 aacagaatcc ttttttaatt attgtttcgt tgtttttggt gtgattcaga aaataaatat
    14461 tttagacagt agccagtcag gcgttaaaaa tggggcggat agtgaggcgg ttttggctgc
    14521 gcttattgaa catttagaat tgatcaatcc ttctggcaga ttaagccaaa atacccgttc
    14581 ggcaatgctt cagttacgcg aagaggaatg gtctgaattc tttttttggt tgctaaattc
    14641 tctggagtgt ctggattatg tgattattaa tttaactcca gaaagcaaaa aaacgttgat
    14701 gagtgaacac cgtaacaata ttcaggtagc aatcgatgct ttatataggc aacgtcggta
    14761 taaatcaccg ggggatgata gtgaaacact tacccgtaga aatgacgcta tcttcggcaa
    14821 ccacgtatgg caaacatttg cgcaatactt cccgccagga cttgaaaaac cgtctgtgta
    14881 gaaaacacca tagctatggt gttatctgcc gacaacgcca tccggagaag ccgaatgacg
    14941 ttgtcggagt aagcgctggt taaggtgtgg gttgtgcctc tttggttgag ggttgcgtcg
    15001 ttgctgactt aacggtcgga cgcgtcgccg gaacattgtc acacggttgc tctttcgggc
    15061 agatcaaatc cagcattttc agcgtcacgc cattggtcca gccaaagcca tcctgtaatg
    15121 gatattcgcc accgccgccc cccgttccgg tggtgctgac atcatatttt tccaccagct
    15181 ttttctcccg gtcataggtg tgctgaacat tggtcaggaa gtgccagcta atgtccatcg
    15241 ccacctcttt ttgcccgtag ttttgtaatc cttctgtcgc gacccactgt aacggtgccc
    15301 agccatttgg cgcatcccat tgttgcccac ttttcaccga cgtggtgttc aggccgccgg
    15361 gttgcagcag atgtgttttc gtcgccgtcg ccattttgtt ggcgcgatct ttcgctgccg
    15421 cattgacgta cagcgggaac agggcggccg cggttaactg attgcgcact ttatgacttt
    15481 tcaggtcgta atcggcatac cagccttgtt gatcgttcca caggtatttt tcgatccctt
    15541 tttgacgggc atttgccagc gtttcgtact ggtttgccat cgcgttatct ccggcagctt
    15601 tgctggcgcg ggcgaggatt ttttccattt taaacatcag gctgttcaga tcgaccggta
    15661 cgatgctggt ggtgcgtaag gtatttaact gctgcgggtt gtccatccag cgcgagctga
    15721 aatcccagcc agacgcagcg gcagagcgca ggtcgcggta aatttcagtg gcaggtcgat
    15781 tcggattgct tttggcggtg gcaatatctt ccacccatga ctctggtcgt ggcgtatcgc
    15841 gatcgtccca gtagcggttg agaagggtac catcctgaag tttgacaacg cgtttttcct
    15901 gttgtccggc ttgcaggttt tcaacaccgt ccatccagta agcatattct ttttgcattt
    15961 gcggcaggta ttgcttcaac gcggcatcgc cttcatgctg cgccagtaac tctaccatca
    16021 tggcaaagaa gggcggttgc gagcggctta aatagtaact gcggttgccg ttgggaatat
    16081 gaccgtaagt gtctatttca tgagcaaaat tggccaccat atccgcgact ttatcccagt
    16141 gaccgctttc ggcaagtcct aacatggtga agtaactgtc ccagtaatat acctcgcgaa
    16201 aacgtccgcc cggcacgaca taaggttccg gcagcggtaa cagagaatcc catttttctg
    16261 tgttttcggt agaacgcgtt aataccggcc aaagtccgtc aatatgttcg cgcagtgact
    16321 gcccctctgg cggaacatat ttctcgcctt ctttcggcag ggtgaaattg acgttaacga
    16381 aatggcgcag atcaaatccg ctctggtttt gctgcatccg ataatcagca aggatcatca
    16441 gcggatcgct gttcggcacg gcatcggcaa aggttttttg gtccggaaaa agtttggcgt
    16501 tttgcacatc attaaacagc ggccctaata aaatatcagg cggctgtggt gttaccggtg
    16561 tttcttctgc ctgcaccgat agcgcagcga aacacaaaaa gatacaggct ggaattaacg
    16621 ccattttttg cgggcgagaa ggtgcggggg atttcatcaa tcattctcct ttggcgaaac
    16681 cgaataaaac ggtattcaag tatcagaaaa ccttagttca ggatcgcact agctgcatga
    16741 tcgaaatctc attctgcgaa ctgtcaggca ttttagaaaa gggaattaaa gggataaata
    16801 acggcgaaat agagtgatca aataacgtct gtttattagc cagccagcgg cgttgggttc
    16861 gtgattagcg aagaaaaatc atcataaatt ggtgaaaaat ataacaggta gttaaaaacc
    16921 attagtgctg agtaaattgc cggatgacat cagaacgatg ccatccgaac agtggcttaa
    16981 ccctgacggt tgaaacgttg ccttttaacg tccagcgtta gcgtttcttc tggttgtatc
    17041 gcatacagtt tctcaccctg ctggcaaatc cagccaatcc ccagttcacg ggcgattagg
    17101 gcgctgtggg ataccggact tccggcgcta aggcagatac tttttacaac cgccggatcc
    17161 agttgcagta ctgtggaagg ataaatgttc tccgccagta gaatagttgg cgagttaaac
    17221 tgcgggagtt cttctttcgt ttgggtcagg tggaccaggg tgcgatgcag aagatcgtcc
    17281 acatcaatat agcgagcttg tagatattca tcatccagtt gctggtattg ctggctaagt
    17341 tctttaagaa cttgctgcca ggcatattct gccgtgcaat gttcatgctg aaggagttcg
    17401 cttgccgccg ccagcagttc cggatcatct aacagtgtat ggtgaccaga aaagattgcg
    17461 gcaatatcgt caagcccgct ggcttctgct ttcgctgtta acgtcatcag atctaataac
    17521 gtgaagtcaa tagcctggcg taatcgatct tgttcttctt ccacggtcag ggttgatttt
    17581 gcctgtaccg tacataaaac tggttgataa taaaaggctt tacccgaaac aggcggaacg
    17641 ggacgcagag taggtggagc gacttcctcc gtttcaccaa agttatcttc agccagctga
    17701 cggaaagcga tcagtgcctc ttcagcttct ggccctttcg caatcaggcg cagcgtatcg
    17761 ttatagcgaa cttgtagtaa cgcaatctgg ttaatactct ctggtgtgac gcatttgccg
    17821 tttttttcca gcaacatatc ggcattaaat gtcgataagg tataaaccag ccgggaggcc
    17881 ggacgtacat gcaggccgtt acggtttttt atgaccaccg ccagagaacg ggcttcttca
    17941 tcgtacgcag gacatgtgtc agagatttta gtgtcggagg acggtaaacc cagttgttca
    18001 cgtttggctt ccagcgcatg catagcgtca aagataactt tgtcgatatc cgctcccgag
    18061 gccgcgctga ccgttgctgc cagtgtacct tcgaccaacg gcgcagcaca caaacgtact
    18121 tttgcggcga tcttgggagc cagcaattcc agcgcagttt cagcactcaa taatgcgcta
    18181 cccatatcca tcatgaccag cacatggtcg gcatcagcaa cagattcgat ggcctccatc
    18241 actttgacgg catcggtacc aataggattt tgtggatcgt caattcccgc ggcaatggcg
    18301 attttacaac tatcactcat taacatctga cgggctaatt caccgacacc ttctcccagt
    18361 cggctgctat gtgaaactat gaccaggttt accatcacca attccttact cttttgcggc
    18421 taacgccaac atttgcatca taaacatcac cgaggtcgcg ccgggatcct ggtgaccaat
    18481 actgcgttca ccgagataac tggcgcggcc tttgcgggct tgcatcgtaa tcgtactttg
    18541 tgcagcggat tcggcgattc tactggcagc ttcgagcgcc accggaacag agagattttg
    18601 ctcgctggac tgacgtaacg attccaccac cggcacccac acatcacaca tggttttatc
    18661 gccaggttcg gctttcccgc gactgattac gccgtccgcg ccatcgcgga acatctgata
    18721 aagctcttcc agtgtcaggc tttgccgtgc ctgggtcgcc tgtgcggcgc ggataaagaa
    18781 ggtaccgaac agcggaccac tggcaccacc gacgctggaa agcagcgtca taccggtatt
    18841 cttgagaatg aaaccgatat ctttatctgc gatagcaggg agtttttcca ccactttgct
    18901 aaagcctcgg ttcatattta gcccgtggtc agcgtcgcca atttcgcgat ccagtccggt
    18961 aagatactcg ctctcggtgc tgaaaatatc gccacaacga gtgagccagt taacaatttg
    19021 agttctgctc agtgacattg ctttctcctt atttacccca gttaagggcc ggggtgtgga
    19081 ccggggcgtc ccagagtgcc agcgtttcgt catcaacttt cagtaaggtg attgagaaac
    19141 cggtcatatc cagtgaggtg cagtacgcgc caattaaatt acgttcgata gtcaatcccg
    19201 cttgctggaa acgtgtggtc aggcggttat agacgccgta cagctcagaa agcggagttg
    19261 cgccaagatt gttaaccagc gcaatcaccc gatcgccaga ctggagcggt tgtttggttt
    19321 gttgttcttc ctgccaactg ccttgttgat aatcccagaa acgcaaagtg cgatggtatg
    19381 agccatttac cagcagggtg tcgaacattt catcgacggt ttgatcaagg gaagagaagg
    19441 ggcggcggtc aatacccggc tcaccatgaa tgccgacgcc aaactccatc tcattatccg
    19501 ccagggtaaa agaaggtttg cccgcggcag gaacggtaca ggcaccgaga gcgataccta
    19561 ttgagtggcc ttgattattc aacttacgcc ccagttccgc acaggcgtcc agtgagtcgc
    19621 cacgctccgc cgctgcgcct acgagttttt caattaatac ggtgttggca acgccgcgtc
    19681 gcccggcagt ataaagactg tcttttaccg caacgtcgtc atcaatgacc acagtggtca
    19741 cttttacgcc gctatcgtgc agtaactcgg tcgctgtttc aaagttaaga atatcgccgg
    19801 tgtaattttt gataatcatc agtacacctt cgccgccatc aatttgcatg gcgcattcaa
    19861 agattttatc gggcgtcggt gaggtgaaaa tttcgcccgg acaggccccc gaaagcatcc
    19921 cctgaccgat ataaccacag tgcatcggct cgtgtccgct gccgccaccc gacagcaggg
    19981 cgacttttcc tgcaacgggg gcatcagctc gggtgacata caccggatcc tgatgcagtg
    20041 tcagagatgg atgcgctttc gccagtcctg ccagttgttc gtccagtacg tcttgcacat
    20101 cattgatcaa ttttttcatt attttgctcc agcaattacg gtagggcatg gatgatgttc
    20161 aacgacacgg cgaccactga ctgccgatga atccattgtg catcaggcaa gggaaaagaa
    20221 aattagccca aattatgttt catagtgaaa catatgcttt aatgaatgtt ccatattgaa
    20281 acttttacgt gtattaatac ttaaaattgc gagccggaac accttttgtc ataagggatg
    20341 cgggatatga gtggcgcttt taacaacgat ggtcggggca tatctccctt aattgcaacc
    20401 tcctgggagc gatgcaataa gctgatgaaa cgggagacat ggaacgtacc acatcaggcc
    20461 cagggcgtga catttgcttc tatttatcgg cgtaagaaag cgatgctgac gctcggtcag
    20521 gctgcgctgg aagatgcctg ggaatatatg gcaccgcgag agtgtgcgct gcttatcctc
    20581 gatgaaaccg cctgcattct cagccgtaat ggcgatccgc aaaccttgca gcagctaagt
    20641 gcactgggat tcaatgacgg cacgtattgc gccgagggaa ttattggtac ttgtgcgcta
    20701 tcgttagcgg ctatctctgg tcaggccgtg aaaacgatgg ccgatcaaca tttcaaacag
    20761 gcactctgga actgggcctt ttgtgcaacg ccgttgtttg acagcaaggg ccgattgacg
    20821 ggaacaatag cgctggcgtg tccggttgag caaactaccg cagctgattt gccgttgacg
    20881 ttggcaatcg cccgcgaggt cggtaattta ctgctgacgg acagtttgct cgctgaaact
    20941 aaccgtcatt taaatcaact taatgccctg ttagaaagta tggatgatgg cgtgattagc
    21001 tgggacgagc agggtaattt gcaatttatt aatgcccagg cggcgcgggt cttgcgcctt
    21061 gacgcgacgg caagtcaggg acgggcaatc actgaactct taacgttacc cgccgtattg
    21121 caacaagcaa taaaacaggc acatccgctc aaacacgtag aagcaacctt tgaaagccag
    21181 caccagttta ttgatgcggt gataaccctt aaaccgataa tagaaacgca gggaaccagc
    21241 tttattttgt tgctccatcc tgtggaacag atgcggcagt tgatgaccag tcaattagga
    21301 aaagtcagcc atacctttgc tcatatgcca caggacgatc cgcaaacccg ccgcttgatt
    21361 cattttggtc gccaggcggc gcgcagtagc tttcctgtcc tgctttgtgg agaagagggc
    21421 gtgggcaagg cactgctaag tcaggcaatt cataatgaaa gcgagcgtgc tgcaggtcct
    21481 tatatcgccg tcaattgtga gttatatggt gatgctgcgc tggcggaaga atttattggt
    21541 ggcgatcgca cggacaatga aaatggccgt ctgagtcggc tggaactggc gcacggcggc
    21601 acgctgtttc ttgaaaagat tgaatatctg gcggtggagt tacagtctgc tttgcttcag
    21661 gttatcaagc agggtgttat cacgcgactg gatgcgcggc gtttaatacc agttgatgtc
    21721 aaagtgattg ccacaacgac cgcggacctc gcaatgctgg tggaacaaaa tcgttttagt
    21781 cgccagctgt attacgcgtt gcatgcattt gaaattacca tcccgcctct gcgtatgcgg
    21841 cgtggcagca ttccggcgct ggtgaataac aaattacgca gtcttgaaaa acgcttctct
    21901 acgcggctga aaattgatga cgatgccctc gctcgcctgg tttcctgtgc atggcctggc
    21961 aacgattttg aactttacag cgtcatcgag aatcttgctc tgagtagtga taatgggcgc
    22021 attcgcgtca gtgatttgcc ggaacatctg tttaccgagc aggcaacaga tgatgtcagc
    22081 gccacacgcc tttccaccag tctgtcattt gcggaagttg aaaaagaggc aattattaac
    22141 gcagcccagg tcacaggcgg tcgcattcag gaaatgtcgg ctttacttgg gatcggccgc
    22201 actacgctgt ggcggaaaat gaagcaacat ggcattgatg cagggcagtt taagcgccgg
    22261 gtatgaaaga cagagacgat ttctgataca tcagagtgat ctatatttca ttccggcgca
    22321 cgctaacatt tttcagcatc gtttaagggc tggtctatcc cgcacttaaa agctgaagcg
    22381 atatcctccg ttgacttgct tctgatcaaa gaggttactc tgcgtgtaat ccgcttcgag
    22441 gtagaatgtg tgctgtttgt tatactgtgc actgactccc acgccgttat tccagccatt
    22501 acctttgaaa ctgtacttct cccgggagtt gttcaacaga tagtctgtat cccctgaaaa
    22561 ctcgcggata gctccagtct tcacatacat attcagctgg ctgttgcctg cggtgatgtc
    22621 atatcccagt atcatgctgg cacgccccag cagcgattcg tagtgattca gatgtatatt
    22681 gagaccatta ctcgccttca tagccatctc attctggtgg ctgtatgtga gctgggtttg
    22741 cggctctata tagaacccat aaccagtagg ggtcaggttg aacctctgcc cggcttccag
    22801 ggagatgctc aatccattcg cagtgccgtt ggctttaacg ccgttgttct gactgtccag
    22861 tacgtgaaaa ctatttttct ggcgcgatgc ttttacaacg agatcgctgt aaaaaccgtt
    22921 gtgtgccatg taactggcat acattcccat gtagtctgaa cgtgcggtac cgtcgcctcc
    22981 gctatagtcc ggcgatgcat gtgttgagcc aatatacaga ccgacataca acggcattac
    23041 atcagagaga cgtttatccc caccaaactg gataccgctg taacccatgt caaagccgct
    23101 cagtttgcca ctggcaaagg agtccaggct tcccccataa ctgcgcaacc agatattacc
    23161 gtctttactc tgatttcgca ggtcacccat ccgttgcatc agcgtacggt tttcaacata
    23221 gttcaataag taaccgacat tgagataatt gccgccagca tctgcggtcg tggtgggttt
    23281 aggcgtggga gcgggttcag gcgtaggatc ggggttgact atgggaggct gagcgggagc
    23341 tggtgtgggt tcaggattag gagtcggttc cggaactgtc ccggaagcgt aaagctccca
    23401 gttagtgcca tttttacgca catcgtacag atatcccccc aactcaacct gagaagacgc
    23461 gctgaacgag gccgcgccgt cagtggtttt taccactgtc agaacttcat ttcccgttgt
    23521 ggcctcgctg ccctggttgc ggatagccaa tacgtgatta ccagcactac tcccgctgat
    23581 attcaataaa tcccctttat tattaacgcc attaccctcg ccaacaacat cagcacgcat
    23641 aataaaggta ctgttaccgc tcaggttctc tacgtttaat gtggcaaatg tgccggcagt
    23701 tgacccgtgg ctggcaaaat cgacagttga atggctcagc gccagcgtgt cgagattaga
    23761 attacttgtt acgttccaga cgctgttatt cattgcaacg tccagttttc cgccattgac
    23821 attatcgctg agggaggaac ctgtccaaac cgacccaggg tgcatatcca gattgattag
    23881 cccacctttg gaaagaacgc taccgttgat aagcatacga cccgaggcgt tgatgcggct
    23941 ggcggcataa ccatcgtcat gttgcgttgc aatggccatc tggtcgggtg tactcatatc
    24001 aatgaccaga tcgctcgtga ggtcgatctg gctgttggtc atggcataaa ttcccctggc
    24061 tcctgccgcg ccggtgatag ccaaactgtc tccggttatt ctaccgccgc tgagcgccca
    24121 caaacccagc gccagactgc cattacgatc aactgtaata tcggtatttt gcatgttgac
    24181 aacagccgtt gccgtctggg ccgaggcacc ataagaaccg ccggaaaaga tgctgtttcg
    24241 ttgcgccgcc gtgccagaaa aattgattgt cgcgtctgaa ccactggtga cgaggccacc
    24301 gccctgcgcg gaagaaatat ggctatctgc accgatagtg gttgtaccac cacgaacttc
    24361 gacgccattg gccgcggctc cagttacatc aacagtgagt gcattcgcgc taacctggcc
    24421 aaagctccag aggccgtgtg cattatcgcc attggtttta atggaactgt ttgttccgag
    24481 atcgacaaca gagtttttct gtacgtttat ccccatggcg ctgtagccct gaacatcgat
    24541 tgtcaggtct gtagccgtaa aacgcgcagc accattggcg ttattgccgt tgagaccacc
    24601 gatataaaca cctgtacttc catcggtcgt gattttactt ccgcttccaa gatcgacact
    24661 ggtgccatag tcattgatgg ttaggcctat accgttcgag ttttcaatgg tgaattgagt
    24721 ggctgttagt gttgagctat gcccaataat gatgccgtca tcgttactct taatggtgct
    24781 gcctgtgcct aagtcagcat gggtatagtc accaattaag ttaatgccga tggcagaggt
    24841 ttgaccaaca acatctactg tgagtcggtt agctgtcagg gagctgtttt tgccctgaat
    24901 aatgattcct tttgcatatc cagaggcgtc gttgacattg acggtgacat cattacccag
    24961 gttgatattg ccaggcgtgt ttcctgctgg ggtcattaca ccatagaggt ttcctgatgg
    25021 atcggcggta tctcctgtaa ttctgtcacc atcattaata gtaatattgg tgccatattg
    25081 acttcctgta tcagcagcta tcagcagggg ggaaaagata attgcattca aaccaatagc
    25141 gccaaatttt atcagttgaa ttgagggcga acggattttt aattcagcga gacgatctgc
    25201 tttggtattc ccattgtgtt gtttgatgcc cataccactc ctatatagta cccagactat
    25261 gggtctatgg attttgtcat tttgtttaaa tcaatgaata atatcctctt atcatcatga
    25321 tatttgtatg gtgcaatcat gatgagcttc tcagagaata attctctatt accgggcaaa
    25381 atcaggaaca caggaattaa tatatattta acatccccct tacaaaggtg ggtttatatc
    25441 gagttattcc ctaagttgtc gttgcttatt ccaattcgtt actataaata tggcaaaaat
    25501 attacaacaa acagcggtta aaaaatttgg tgaagatttt ttgtaataat cctaattagt
    25561 tatgggaatt tcgcttgatg tatctgcata acaaaaacaa tatgcgccat ttttgcatta
    25621 ctaataagaa aaagcaaaaa acatcgatat tcatttttgg tcaatgggtt ttctggccta
    25681 tcgttgttat tgtgctactg gttatcattt tcatcttgcc aggatcgcac gtaatcacgt
    25741 tattcgggga ctttatctgc gggatattac ccttcaggta agtccttttc tcgccagcat
    25801 attggagcgt gatcaatatt gatcagctgt gaagggggtg atgtgtagtg gaccaaaact
    25861 tacgttatat cactactaat gagatatccg tcatctgaca ctgatgtctt taattattct
    25921 cgtggttcac tgtaggcaat aagcacaaaa gtgtaggatg ttacaagaat gattaggact
    25981 cggtgaaatg aaaaatccac gcaattgcgt ggattttata tacttttgcg ctcttcatga
    26041 gatttagcga aacctcatga gacaataaat taattagacg ttgaaaagga agttcatcac
    26101 atcgccatct ttcacgatgt aatctttacc ttctgcacgc attttgcctg cttctttcgc
    26161 gccttgttca cctttgtaag tgatgaaatc ttcaaacgag atggtttgtg cacggataaa
    26221 gcctttttca aaatcagtat ggattttgcc cgctgcctgc ggcgcggttg ctccaaccgg
    26281 aatggtccat gcacgcactt ctttcacccc agcggtgaag taagtttgca ggttcagcag
    26341 cttataaccg gcacggatca cacggttcag gcccggctct tccagcccaa gctcctgcat
    26401 aaactcgtca cgttcttcgt cgtccagttc ggcaatgtct gcttcaacag cagcacaaac
    26461 cggaaccaca acagaacctt ctttcgccgc gatttcacgc acctggtcaa gatatgggtt
    26521 gttttcaaaa ccgtcttcgt tgacgttggc gatgtacatt gttggtttca gcgtcaggaa
    26581 gctcaggtaa cgaatagccg ctttctcttc agcgcttaaa tccagcgcgc gcagcatacc
    26641 tgcattttcc aactggggca ggcatttttc caggaccgcc agctcagctt tcgcgtcttt
    26701 atcgccacct ttggctttct tctgtacgcg atgaatcgca cgttcgcagg tgtcgaggtc
    26761 tgccagcgcc agttcggtgt tgataacttc aatatcgtca gccgggttaa ctttgccgga
    26821 aacgtgaatg atgttgtcat tttcaaagca gcgaacaacg tgaccgatcg cttcggtttc
    26881 acggatgttg gtcaggaact ggttacccag accttcgcct ttcgatgcgc cttttaccag
    26941 accggcgata tcgacaaatt ccatggtcgt gggaagcgta cgttgcggtt ttacgatttc
    27001 agccagttga tccaggcgag ggtcaggcat tggtacgacg cctgtgttcg gctcaatggt
    27061 gcagaatgga aagttggccg cttcaatacc ggctttggtc agcgcgttga acagggtaga
    27121 tttcccgacg ttgggcaaac cgacgatacc gcatttgaat cccatgattt aaatcacctt
    27181 aatatcttaa taatcaacct gttattgcta acagattgca gaaatggaaa taactttgcc
    27241 tattatacac ggcactcggc aaaaatgccg cagacaacga cttattgcgc tttaaaggcg
    27301 tgcaatcggt tcgttgcttt ggtcaagcca tctgtaaacc acatttcagt acaacgcgcc
    27361 gcttcgtcga tggcttcatc aattaacttc tgttcactaa caggcggttt gcctaacaca
    27421 aaaccgacaa ctttattttt atcgcccgga tgaccgattc cgatgcgtaa acggtgaaag
    27481 ttagggttat tacccaattt actgatgatg tctttcagtc cattgtgacc accatggcca
    27541 ccgcccaatt taaatttggc gacgccagga ggcagatcca gttcgtcgtg ggccaccaga
    27601 atttcgtccg gattaatgcg gaaaaaactg gccatcgccg caacggcttt gccgctgaga
    27661 ttcataaatg tagtcgggac taacaggcgg acatcttcgc ctccaagagt gactcgcgaa
    27721 gtataaccaa agaatttagc ctcttcgcgc agcggagcgc gcaaacgctc tgccagtaag
    27781 tcaacgaacc aggcaccagc attatgtcgc gttgcggcgt attcagcacc ggggttcgcc
    27841 aggccgacaa tcaatttaat cgtcacgttt ttttgtcctg agtgtgtaca taactggcgc
    27901 gtagtttact ggttgcggcc ccgcttgaca aaaaactgcg tatcaaatgc agataacgta
    27961 ataattgcct gagtggacta ttagaaagtc aaggtgttca ggcgtttatt tgtaaagttt
    28021 tgttgaaata agggttgtaa ttgtgatcac gccctcacat aacccacagg gtgttgtcta
    28081 tactttacac ataaggaaga ggggtattcc ctgttacaac ccagaaagtt ccggaggtga
    28141 catatgaaac gcaaaaacgc ttcgttactc ggtaacgtgc tcatggggtt gggtctggtg
    28201 gtaatggtgg tcggcgtggg gtattcaatc ctcaaccagt taccacagtt taatatgccc
    28261 cagtatttcg cacatggtgc agtgctaagt attttcgtcg gtgccattct ctggctggcg
    28321 ggtgcccgtg ttggcgggca tgaacaggtg tgcgaccgtt actggtgggt tcgccactat
    28381 gacaaacgtt gccgccgtag cgataatcgc cgtcatagct aacaaataat gcagtttgct
    28441 gaccagtcag tttgcgctga ctggtcaatc tcgtacttat aaatctgcca tcgccgcgcg
    28501 acgattcggg aagaacgcca ggcgtcccgg gatcggttga atgccagcgc gagccatagt
    28561 gcgcagtggc tggaattcca cgttgcacac gcgcagttca catccctcgg gcagacgctt
    28621 cacaaaacgc tggaacgcat caagaccacc agcatcaagt accggaaccg catcccactt
    28681 cagaatcaca atccgtttgc cttcaagacg tgactccagg tccgtgaaca agccttctgc
    28741 agcagcaaaa aacagcgggc caataacgcg cagtaccaga acatcgtctg gaacatctac
    28801 gactaccggt gccaggcgag tcatacgtgc gatacgacgc ataaacagca gcgatgccag
    28861 cacgatcccc acgctgatgg caataaccat atcaaacagc acggtcagcg acatgcacag
    28921 cagcatgaca atgatgtcat ctttcggtgc atgacgcagc aagtcgacca ctttatgcgc
    28981 ttcactcatg ttccacgcca ccatcaacag cagggctgcc atagcggaaa gcggcagcca
    29041 ggagagcagc ggtgccagta ccagcagggc aagaataacc agaatagagt ggatcaccgc
    29101 tgagataggg gaagttgccc cggcacggac gttagcggca gaacgcgcga tggcagctgt
    29161 agcggtaata ccaccaaaga acggagcgat aatattcccc agtccctgtc caaccagttc
    29221 gctgttcgcc ttgtgtttcg tcccggtcat accatccagt accacggcgc agagcagaga
    29281 ttcgattgcg ccgagcattg ccattgagaa tgccgcaggc agcagtgtgc gaatagaatc
    29341 ccaggttagc gtgaattctg aattaggcag atcccacggc agcaccagtt gcggcagcag
    29401 ttgcggaata ccgttaccct gagaaccatc ggccaggacg tagtggaatt gcgaaccgat
    29461 ggtagcaaca tgtccgccga gcaggttaac aatccccatc accgcgcaac cagccagcaa
    29521 ggccggaagg tgaccgggta aacgaatgcc cagacgcggc cagaaaacaa gaatacctag
    29581 cgtcacaatg ccaatggcag catcacccac attaatggtc ggcagcgcca taaataatgc
    29641 gccgactttt tgtagataat gttccgggac atgggccatt tgcagaccga gaaaatcttt
    29701 aatctgcatg gtaccgatgg tgatcccgat acccgaggtg aaacctaagg tgacggaaac
    29761 cggaatatac tcaatcaggc gaccaaagcg tgccagaccc atcagaatca aaaaaatccc
    29821 cgacagcaag gtcgcaacca gcagtcctgc cagtccaaac tgttgcgaca cgggatagag
    29881 aattaccaca aatgccgcag tcggaccgga aacgctaaag cgtgacccac ccgtcagagc
    29941 aatgacaatc cccgcaacag ctgcggtata taaaccgtac tggggtgcca caccactacc
    30001 aatagccaac gccatcgcca gcgggatagc aataatcccg acggttatcc cggcaatcag
    30061 gtcacgggta aaccgtgcgg cagtatattt ttctttccag caagcgtcga tcagagcgcg
    30121 gaaaggcatc acatgtgagg aaaatatttt gttcacaata atgtttcatc cgtgagcgca
    30181 tcatctgtca actaaatggc aggtgaagga ggcataggtc atacaaatgg atattacaga
    30241 caaaaaaacc cgccgcagcg ggtctttgag ccgggttcga ttagtgttcg aacatggcag
    30301 agatcgattc ttcgttgctg atacgacgaa tcgcttcggc cagcatacct gacagggtca
    30361 gagtacgcac gttcggcagt gatttgattt catcgctcag cggaatggta tcgcagacaa
    30421 cgacttcatc aattacagag ttacgcaggt tattcgccgc gttgccagag aagatcgggt
    30481 gagtcgcgta cgcaaataca cgtttagcac cacgttcttt cagagcttca gcagctttac
    30541 acagcgtacc gccagtgtcg atcatatcat cgaccagtac gcagtcacga cctgcaacgt
    30601 caccgatgat atgcatcacc tgggaaacgt tcgcacgcgg acgacgtttg tcgatgattg
    30661 ccatatcggt atcgttcagc agcttagcga tagcgcgggc acgcacaacg ccgccgatgt
    30721 ccggagaaac cacaattggg ttatccagat tcagctgcag catgtcttcc agcaggatcg
    30781 ggctaccaaa tacgttatca accggaacgt cgaagaaacc ctgaatctgt tcagcgtgca
    30841 gatccactgt cagcacacgg tcaacaccga cgctggagag gaagtctgca accactttcg
    30901 cagtgattgg tacacgagcg gaacggacgc gacggtcctg gcgcgcatag ccaaagtagg
    30961 ggataacagc ggtgatacga cctgcggaag cacgacgcag ggcatcaacc ataacgacta
    31021 attccatcag gttgtcgtta gtaggggcac aagtggactg gatgatgaaa atatcaccac
    31081 cgcgtacatt ttcgttgatt tgtacactga cttcgccgtc gctaaagcga cctacagcgg
    31141 cgtcgccgag tgaagtgtac aggcggttgg caatacgttg tgctagttcc ggggtggcgt
    31201 taccagcaaa aagcttcata tcaggcacga gaagaacctc aggcatgcgt ccattggtgg
    31261 aaagaatctg ccgaaaactg tgcgggccag gcatgatcct ttccaggcgg tgtattaaag
    31321 agcgcgatgc aacgtctgga acaaggtgac gttgtcaccg aaactcagct tgcccggctt
    31381 aaagcatggc tctgtgcaat ggggaaagat taacgccttt cgccacaaag ccattgagcc
    31441 attccggggc ttgctctagc acctggcggg cttcagactc tgtatcaaat tcagcaaaga
    31501 cacaggcccc tgtcccagtc aggcgcgacg gggcgtattc taacagccag gaaagcaccg
    31561 catcaacctc gcgaaaacgt tttcttgcga taacctcgca atcattgctg aattcacatt
    31621 ttagcaacgt ttctattgac ctttttggcg tattgcgcgg gagttcagga tctttaaaaa
    31681 tcaccggagt cggaatactt acaccagggt gcgccaccag ataccacttc tctggcggat
    31741 ccaccggcgt tagtatttca ccaacgcctt cggcaaacgc ggcatgcccc cgaacaaaga
    31801 caggaacatc tgcgcccagc gtcagcccca tttccgccag ctcatccatg cttagcccgc
    31861 attgccagag atgatttaat gccaccagga ccgtcgcggc attggatgaa ccaccgccga
    31921 gaccgccgcc catcggcaaa cgcttgtcaa tgctgatatt cgcaccgctt cccgtcggaa
    31981 gacgcccgct gtctgccgca gtttttatca acaatcgcgc tgcgcgaacg atcaggttat
    32041 cttcatgttc cacgccttca acgggcgtta acagacgaat atccccatcg tcacgaagct
    32101 caatgctgat ggtgtcgccg taatcaagaa actgaaacag cgtttgcagc gtgtggtaac
    32161 catccgcacg ctgaccggta atgtataaaa acagattaag ttttgccgga gagggccact
    32221 gtgtccgcat tatttcacta tccagttatc catttttaac ttgatgcgtt gaccaccgtc
    32281 ggtgagttcc atattggctg gcatcgcagg ttgcgttttg gtgtcataac caccataaac
    32341 aaccttccag tttttgccat tctggctgta ggtaatttcg ctcaggcggt actggtcgtc
    32401 cagtttgtag tcggttgcat cacccggtaa acctaaaatc cactggcgca agctgttgag
    32461 cggaattggc attccggtca atttgccaat catctcttcg gcgtcatcgg cggtataacg
    32521 ctgaccttta ttgtcgacta actgcacgtt acccggttga gcattcagct ccagttccgt
    32581 gctgcccaat gggttagtga gcagcagacg gtagcgatcc tggccggttt gctgccagaa
    32641 aaagcgggcg tacacttttt gttggtcaga aatataagcg aacgcgccgc gagtctgata
    32701 ctgattaaga ttgcgcacgt cttgctgatg ctgacgccat tgtggcgaat ccgggctttt
    32761 gccaggacct ttgggcgtgg taacggaaca ggcagtgagc acaagagcag ccagcggtag
    32821 caggcggata agacgaaaat cgggcagggg catagtgatg acaagtcctt gagatacgtt
    32881 gcagttataa cccttaatgc tagcgttacc gtccgctatc gtctatgttc aagttgtctt
    32941 aattgccaga atctaacggc tttcggcaat tactccaaaa gggggcgctc tcttttattg
    33001 atcttacgca tcctgtatga tgcaagcaga ctaaccctat caacgttggt attatttccc
    33061 gcagacatga cccttttagc actcggtatc aaccataaaa cggcacctgt atcgctgcga
    33121 gaacgtgtat cgttttcgcc ggataagctc gatcaggcgc ttgacagcct gcttgcgcag
    33181 ccgatggtgc agggcggcgt ggtgctgtcg acgtgcaacc gcacggaact ttatcttagc
    33241 gttgaagagc aggataacct gcaagaggcg ttaatccgct ggctttgcga ttatcacaat
    33301 cttaatgaag aagatctgcg taaaagcctc tactggcatc aggataacga cgcggttagc
    33361 catttaatgc gtgttgccag cggcctggat tcactggttc ttggggagcc gcagatcctc
    33421 ggtcaggtta aaaaagcgtt tgccgattcg caaaaaggtc atatgaaggc cagcgaactg
    33481 gaacgcatgt tccagaaatc tttctctgta gcgaaacgcg ttcgcactga aacagatatc
    33541 ggtgccagtg ctgtgtctgt cgcttttgcg gcttgtacgc tggcgcggca gatctttgaa
    33601 tcgctctcta cggtcacagt gttgctggta ggcgcgggcg aaaccatcga gctggtagcg
    33661 cgtcatctgc gcgaacataa agtacagaag atgattatcg ccaaccgcac tcgcgaacgt
    33721 gcccaaattc tggcagatga agttggcgcg gaagtgattg ccctgagtga gatcgacgaa
    33781 cgtctgcgcg aagccgatat catcatcagt tccaccgcca gcccgttacc gattatcggg
    33841 aaaggcatgg tggagcgcgc attaaaaagc cgtcgcaacc aaccaatgct gttggtggat
    33901 attgccgttc cgcgcgatgt tgagccggaa gttggcaaac tggcgaatgc ttatctttat
    33961 agtgtggacg atctgcaaag catcatttcg cacaacctgg cgcagcgtaa agccgcagcg
    34021 gttgaggcgg aaactattgt cgctcaggaa accagcgaat ttatggcgtg gctgcgagca
    34081 caaagcgcca gcgaaaccat tcgcgagtat cgcagccagg cagagcacgt tcgcgatgag
    34141 ttaaccgcca aagcgttagc ggcccttgag cagggcggcg acgcgcaagc cattatgcag
    34201 gatctggcat ggaaactgac taaccgcttg atccatgcgc caacgaaatc acttcaacag
    34261 gccgcccgtg acggggataa cgaacgcctg aatattctgc gcgacagcct cgggctggag
    34321 tagcagtaca tcattttctt tttttacagg gtgcatttac gcctatgaag ccttctatcg
    34381 ttgccaaact ggaagccctg catgaacgcc atgaagaagt tcaggcgttg ctgggtgacg
    34441 cgcaaactat cgccgaccag gaacgttttc gcgcattatc acgcgaatat gcgcagttaa
    34501 gtgatgtttc gcgctgtttt accgactggc aacaggttca ggaagatatc gaaaccgcac
    34561 agatgatgct cgatgatcct gaaatgcgtg agatggcgca ggatgaactg cgcgaagcta
    34621 aagaaaaaag cgagcaactg gaacagcaat tacaggttct gttactgcca aaagatcctg
    34681 atgacgaacg taacgccttc ctcgaagtcc gagccggaac cggcggcgac gaagcggcgc
    34741 tgttcgcagg cgatctgttc cgtatgtaca gccgttacgc cgaagcccgc cgctggcggg
    34801 tagaaatcat gagcgccagc gagggtgaac atggtggtta taaagagatc atcgccaaaa
    34861 ttagcggtga tggtgtgtat ggtcgtctga aatttgaatc tggcggtcat cgcgtgcagc
    34921 gtgttcctgc tacggaatcg cagggtcgta ttcatacttc tgcttgtacc gttgcggtaa
    34981 tgccagaact gcctgacgca gaactgccgg acatcaaccc agcagattta cgcattgata
    35041 ctttccgctc gtcaggggcg ggtggtcagc acgttaacac caccgattcg gcaattcgta
    35101 ttactcactt gccgaccggg attgttgttg aatgtcagga tgaacgttca caacataaaa
    35161 acaaagcgaa ggcgctttca gtgctcggtg ctcgcatcca cgctgctgaa atggcaaaac
    35221 gccaacaggc cgaagcgtct acccgtcgta acctgctggg gagtggcgat cgcagcgacc
    35281 gtaaccgtac ttacaatttc ccgcaggggc gcgttaccga tcaccgcatc aacctgacgc
    35341 tctacagcct ggatgaagtg atggaaggta agctggatat gctgattgaa ccgattatcc
    35401 aggaacatca ggccgaccaa ctggcggcgt tgtccgagca ggaataatgg aatatcaaca
    35461 ctggttacgt gaagcaataa gccaacttca ggcgagcgaa agcccgcggc gtgatgctga
    35521 aatcctgctg gagcatgtta ccggcagagg gcgtactttt attctcgcct ttggtgaaac
    35581 gcagctgact gacgaacaat gtcagcaact tgatgcgcta ctgacacgtc gtcgcgatgg
    35641 tgaacccatt gctcatttaa ccggggtgcg agaattctgg tcgttgccgt tatttgtttc
    35701 gccagcgacc ttaattccgc gcccggatac ggagtgtctg gtggagcagg cactggcgcg
    35761 gttgcctgaa caaccttgcc gtattcttga tctcgggacg ggtaccgggg cgattgcgct
    35821 tgcgctggct agcgagcgcc cggactgcga aattatcgct gtagatcgta tgcctgatgc
    35881 tgtctccctg gcacaacgta atgcccagca tctggcgatc aaaaatatcc acattctgca
    35941 aagcgactgg tttagcgcgc tagccgggca gcagtttgcg atgattgtca gcaatccgcc
    36001 gtatattgac gagcaggacc cacatcttca acaaggcgat gtccgctttg agccgctcac
    36061 tgcgctggtt gcggcagaca gtggaatggc agacatcgtg catatcatcg aacagtcgcg
    36121 taacgcgctg gtatccggcg gctttctgct tctggaacat ggctggcagc agggcgaagc
    36181 ggtgcgacaa gcatttatcc tcgcggggta tcatgacgtc gaaacctgcc gtgactatgg
    36241 tgataacgag cgcgtaacgc tcggccgcta ttatcaatga caagtttttc tacactgctt
    36301 agtgttcatc ttattagtat cgcgctttct gttgggctat taaccttacg tttctggcta
    36361 cgttatcaga agcatccaca ggcatttgct cgctggacgc gcattgtgcc gccggttgtc
    36421 gatacggtgt tattgttaag cggcattgcg ttgatggcta aagcgcacat cctgccattt
    36481 tccgggcagg cacagtggct gactgaaaag ctgtttggag ttatcattta tatcgttttg
    36541 ggttttattg cactcgatta tcgtcgtatg cacagtcagc aggcgcgcat tattgccttc
    36601 ccgctggcgt tggtggtgct gtacatcatc attaaactcg ccaccacaaa agtaccgtta
    36661 ctggggtaag tcatgagatc gttagctgat ttcgaattta ataaagcgcc attgtgcgaa
    36721 ggcatgatcc tggcttgcga agcaatccgc cgcgattttc cctcgcaaga tgtttacgac
    36781 gaactggagc gtctcgttag tctggcgaag gaagaaatca gccagcttct gcctttagaa
    36841 gagcagctgg aaaaactgat cgcgctgttt tacggcgact ggggatttaa agcctcacgc
    36901 ggcgtttatc gtctttccga tgcattatgg ctggaccagg tgttaaagaa tcgacagggc
    36961 agtgcggtat cattaggtgc ggttttatta tgggtcgcga atcgtctcga tttgccgctg
    37021 ctgccggtga ttttccctac gcagctgata ttgcgcattg aatgtccgga tggcgaaatt
    37081 tggctgatta atccttttaa cggtgaatcg ttaagcgaac atatgctgga cgtatggtta
    37141 aagggaaata tcagcccgtc ggcggaactg ttttatgaag accttgatga agctgataac
    37201 attgaggtaa tccgcaaatt gctggataca ctcaaagcct cgttgatgga agaaaatcag
    37261 atggagctgg cgttacgcac cagcgaagct ttattacaat tcaaccctga agatccctat
    37321 gaaattcgcg atcgcgggtt gatttatgcg caactggatt gcgaacacgt tgcgttgaac
    37381 gatttaagtt atttcgttga acagtgtccg gaagacccga tcagcgaaat gatccgtgcg
    37441 caaataaata acatcgcgca taaacatatt gtgctgcatt aattaatcga cattttactc
    37501 aagattaagg cgatcctatg aaacaaaaag tggttagcat tggcgacatc aacgtagcaa
    37561 atgacctgcc gttcgtactg tttggcggta tgaacgtgct ggaatctcgc gatctggcga
    37621 tgcgcatttg cgagcactac gtaactgtga cccagaaact gggtatccct tacgtgttca
    37681 aagcctcttt tgacaaagcc aaccgctcct ccatccactc ttatcgtgga ccgggcctgg
    37741 aagaagggat gaaaatcttc caggagttga agcagacttt tggcgtgaaa attatcaccg
    37801 acgttcacga accaagtcag gcacagcccg ttgctgatgt cgtggatgtg attcagttgc
    37861 cggcgtttct tgctcgccag actgacctgg ttgaagccat ggcgaaaacc ggtgcggtaa
    37921 ttaacgtcaa gaaaccacag tttgtcagcc cgggacagat gggtaatatc gttgataaat
    37981 tcaaagaagg cggcaacgaa aaagtgattc tttgcgatcg tggtgctaac ttcggctatg
    38041 acaacctggt tgtcgatatg ctgggcttca gcatgatgaa gaaagtgtct ggtaactcgc
    38101 cggtgatttt cgacgtgacc cacgcactgc aatgccgcga tccgtttggc gcagcttccg
    38161 gtggtcgtcg tgctcaggtg gctgagctgg cacgagccgg tatggcggta ggtctggcgg
    38221 ggctgtttat tgaagcgcat ccggatccgg aacatgcgaa atgtgatggt ccatccgcgc
    38281 tgccgctggc taaactggaa ccgttcctca agcagatgaa agcgattgat gatctggtga
    38341 aaggtttcga agaactggat accagcaagt aatctttttt gcttgaaaaa taaagtctta
    38401 gcgttctgcg ttaagacttt tttcatgggt gccagataca aaaaaggccg caggctgtta
    38461 cccctgcggc cggtttcggg cgcatattgc catcacggca gcctgacgcc cgttttcacc
    38521 ttacttccgg ttacgccacc agccgacaat cgctgcggta ataattcccg ccaggatcgg
    38581 tgctgccagg tcgtgccaga aaatcatggc aaactgcgcg agcgtcatat agccgccttg
    38641 ttgtaatgac aacattttgc ggctattc
//
LOCUS       DAZGVQ010000027        38327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000027 DAZGVQ010000000
VERSION     DAZGVQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.027"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..614
                     /gene="acrS"
                     /locus_tag="ACHLAG_001366"
     CDS             <1..614
                     /gene="acrS"
                     /locus_tag="ACHLAG_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFP0654626.1"
                     /translation="ETAIAQFAQHGVSKTTLNDIADAANVTRGAIYWHFENKTQLFNE
                     MWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAKIPRQQALLKILYHKCEF
                     NDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLDVVMIIINGAFSGIVQNW
                     LMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELSVM"
     gene            complement(617..796)
                     /locus_tag="ACHLAG_001367"
     CDS             complement(617..796)
                     /locus_tag="ACHLAG_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFP0654627.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(880..1764)
                     /gene="yhdJ"
                     /locus_tag="ACHLAG_001368"
     CDS             complement(880..1764)
                     /gene="yhdJ"
                     /locus_tag="ACHLAG_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP0654628.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1850..2146)
                     /gene="fis"
                     /locus_tag="ACHLAG_001369"
     CDS             complement(1850..2146)
                     /gene="fis"
                     /locus_tag="ACHLAG_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFP0654629.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2172..3137)
                     /gene="dusB"
                     /locus_tag="ACHLAG_001370"
     CDS             complement(2172..3137)
                     /gene="dusB"
                     /locus_tag="ACHLAG_001370"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFP0654630.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(3466..4347)
                     /gene="prmA"
                     /locus_tag="ACHLAG_001371"
     CDS             complement(3466..4347)
                     /gene="prmA"
                     /locus_tag="ACHLAG_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFP0654631.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4359..5810)
                     /gene="panF"
                     /locus_tag="ACHLAG_001372"
     CDS             complement(4359..5810)
                     /gene="panF"
                     /locus_tag="ACHLAG_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFP0654632.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5800..6042)
                     /locus_tag="ACHLAG_001373"
     CDS             complement(5800..6042)
                     /locus_tag="ACHLAG_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFP0654633.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6151..7500)
                     /gene="accC"
                     /locus_tag="ACHLAG_001374"
     CDS             complement(6151..7500)
                     /gene="accC"
                     /locus_tag="ACHLAG_001374"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFP0654634.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7511..7981)
                     /gene="accB"
                     /locus_tag="ACHLAG_001375"
     CDS             complement(7511..7981)
                     /gene="accB"
                     /locus_tag="ACHLAG_001375"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFP0654635.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8959..9933)
                     /locus_tag="ACHLAG_001376"
     CDS             complement(8959..9933)
                     /locus_tag="ACHLAG_001376"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFP0654636.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10085..12025
                     /gene="csrD"
                     /locus_tag="ACHLAG_001377"
     CDS             10085..12025
                     /gene="csrD"
                     /locus_tag="ACHLAG_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFP0654637.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            12330..13373
                     /gene="mreB"
                     /locus_tag="ACHLAG_001378"
     CDS             12330..13373
                     /gene="mreB"
                     /locus_tag="ACHLAG_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFP0654638.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            13439..14542
                     /gene="mreC"
                     /locus_tag="ACHLAG_001379"
     CDS             13439..14542
                     /gene="mreC"
                     /locus_tag="ACHLAG_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFP0654639.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAVPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            14542..15030
                     /gene="mreD"
                     /locus_tag="ACHLAG_001380"
     CDS             14542..15030
                     /gene="mreD"
                     /locus_tag="ACHLAG_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFP0654640.1"
                     /translation="MASYRSQGCWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15039..15632
                     /gene="yhdE"
                     /locus_tag="ACHLAG_001381"
     CDS             15039..15632
                     /gene="yhdE"
                     /locus_tag="ACHLAG_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFP0654641.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15622..17091
                     /gene="rng"
                     /locus_tag="ACHLAG_001382"
     CDS             15622..17091
                     /gene="rng"
                     /locus_tag="ACHLAG_001382"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFP0654642.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            17159..20959
                     /gene="yhdP"
                     /locus_tag="ACHLAG_001383"
     CDS             17159..20959
                     /gene="yhdP"
                     /locus_tag="ACHLAG_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFP0654643.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTSVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVEMNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGDITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21115..22560
                     /gene="tldD"
                     /locus_tag="ACHLAG_001384"
     CDS             21115..22560
                     /gene="tldD"
                     /locus_tag="ACHLAG_001384"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFP0654644.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVSGTA"
     gene            complement(22688..23617)
                     /gene="aaeR"
                     /locus_tag="ACHLAG_001385"
     CDS             complement(22688..23617)
                     /gene="aaeR"
                     /locus_tag="ACHLAG_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFP0654645.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23800..24003
                     /gene="aaeX"
                     /locus_tag="ACHLAG_001386"
     CDS             23800..24003
                     /gene="aaeX"
                     /locus_tag="ACHLAG_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFP0654646.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24011..24943
                     /gene="aaeA"
                     /locus_tag="ACHLAG_001387"
     CDS             24011..24943
                     /gene="aaeA"
                     /locus_tag="ACHLAG_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFP0654647.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24949..26916
                     /gene="aaeB"
                     /locus_tag="ACHLAG_001388"
     CDS             24949..26916
                     /gene="aaeB"
                     /locus_tag="ACHLAG_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFP0654648.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27008..27280
                     /locus_tag="ACHLAG_001389"
     CDS             27008..27280
                     /locus_tag="ACHLAG_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFP0654649.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQAFGLAKHKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(27336..27599)
                     /gene="yhcN"
                     /locus_tag="ACHLAG_001390"
     CDS             complement(27336..27599)
                     /gene="yhcN"
                     /locus_tag="ACHLAG_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFP0654650.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27964..28434)
                     /gene="argR"
                     /locus_tag="ACHLAG_001391"
     CDS             complement(27964..28434)
                     /gene="argR"
                     /locus_tag="ACHLAG_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFP0654651.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28869..29807
                     /gene="mdh"
                     /locus_tag="ACHLAG_001392"
     CDS             28869..29807
                     /gene="mdh"
                     /locus_tag="ACHLAG_001392"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP0654652.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQSVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            complement(29870..30937)
                     /gene="degS"
                     /locus_tag="ACHLAG_001393"
     CDS             complement(29870..30937)
                     /gene="degS"
                     /locus_tag="ACHLAG_001393"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFP0654653.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(31027..32394)
                     /gene="degQ"
                     /locus_tag="ACHLAG_001394"
     CDS             complement(31027..32394)
                     /gene="degQ"
                     /locus_tag="ACHLAG_001394"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFP0654654.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            complement(32548..32946)
                     /gene="zapG"
                     /locus_tag="ACHLAG_001395"
     CDS             complement(32548..32946)
                     /gene="zapG"
                     /locus_tag="ACHLAG_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFP0654655.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            33140..34267
                     /gene="zapE"
                     /locus_tag="ACHLAG_001396"
     CDS             33140..34267
                     /gene="zapE"
                     /locus_tag="ACHLAG_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFP0654656.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            34486..34914
                     /gene="rplM"
                     /locus_tag="ACHLAG_001397"
     CDS             34486..34914
                     /gene="rplM"
                     /locus_tag="ACHLAG_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFP0654657.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34930..35322
                     /gene="rpsI"
                     /locus_tag="ACHLAG_001398"
     CDS             34930..35322
                     /gene="rpsI"
                     /locus_tag="ACHLAG_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP0654658.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35717..36355
                     /gene="sspA"
                     /locus_tag="ACHLAG_001399"
     CDS             35717..36355
                     /gene="sspA"
                     /locus_tag="ACHLAG_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFP0654659.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            36361..36858
                     /gene="sspB"
                     /locus_tag="ACHLAG_001400"
     CDS             36361..36858
                     /gene="sspB"
                     /locus_tag="ACHLAG_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFP0654660.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36901..38268)
                     /gene="dcuC"
                     /locus_tag="ACHLAG_001401"
     CDS             complement(36901..38268)
                     /gene="dcuC"
                     /locus_tag="ACHLAG_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP0654661.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIHSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9314 a         9577 c        10066 g         9370 t
ORIGIN      
        1 ttgaaactgc catcgcccag tttgcgcagc atggcgtaag caagacgaca ctcaacgaca
       61 ttgccgacgc tgctaacgtt acgcgtggcg ctatctactg gcacttcgaa aacaagactc
      121 aactgtttaa tgagatgtgg ttgcaacagc cttcattgcg ggagttaatc caggaacact
      181 tgacggctgg attagagcat gacccgtttc aacaattgcg tgaaaaattg attgtcggct
      241 tgcaatatat tgccaaaatt ccccgccagc aggcgttgct gaaaatctta tatcacaaat
      301 gtgaatttaa tgatgagatg ctggccgagg gagtgatacg cgaaaagatg ggcttcaatc
      361 cgcagactct ccgcgaagta ttgcaggcgt gtcagcaaca aggttgtgta gcaaataacc
      421 tcgatttaga tgttgtgatg attattatta atggtgcctt cagcggaatt gttcaaaact
      481 ggttaatgaa tatggcgggt tatgatcttt ataaacaagc ccccgctctg gtcgataacg
      541 tattaagaat gttcatgcca gatgaaaaca taacgaaatt aattcatcaa acgaatgaat
      601 taagtgtcat gtaaattcac attctgtcct gaaaattcag ttcgtcacag ttaaccagtt
      661 ttagtggatt aactgagttc atattgcggc atttgtctgt ttctgttgcc agcagttcaa
      721 tccactgcat cagtaaagta tcaaacagaa aaacgaagac agcgaaaacg acgagccacc
      781 aatacttgcg aatcattacc tgagcctgtg tatgtttaac caagtctggc aggaaatata
      841 cacgaaatga ataatctgaa aaccaggctt acacttcctt tactttgcaa tgagatcggg
      901 gtcaacttcg cttaaccggc ttcgttttga caggttgccg gtctttcttt ttttcacttt
      961 cgccagttct tccgcagagt aatgcgacgc gacatccagc cgtcgaagcc ccattttgat
     1021 gtactcgctg ttgatctcaa taccaatgaa ttttcgcccg ctggagacgg ctacggcacc
     1081 ggtagtaaag ctaccggcaa acgggtcgag aacgatatcg cctgggttgg aagaggcgag
     1141 aataatgcgt ttcagtaagg cttccggttt ttgcgtcggg tggttctcat attcatccat
     1201 caaataacgc acgcgcggaa aatcccagac gttacccggt actttttgat gattgtatgg
     1261 ctgtggagga tttttgcgat aatcgatcaa cgcgcgctgc gatccggttt tggcttctac
     1321 cagaatagca tcaccgttga atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg
     1381 ttcgtacatg gagccgtagt gttttttcgc ctgtactcca gaactgtcat atgaccagac
     1441 gatgcgactt ttgatggtaa aaagcttgcg gcactggaga tcgataaagg gcatgttttc
     1501 cgtgctgttc atgatgtaca tgctgccctg ctttttcaga acgcggtggc actctgcaat
     1561 cacttcaaac agccagtcga taaacagatc ttctttccag gcttcgatca gaccatcaaa
     1621 atttttaccg atattatatg gtgggtcggc aaagatcaga tcgacacttt cggcggggat
     1681 ctttttaagt tcggcaagcg catcaccgtg aataatggtc ttagcttcat taccaaaccg
     1741 ggtcggttca catcctgttc tcatggtcac tccctttgtg acacctataa aaaaggcgct
     1801 tccccatgcc gagtagcgcc tttttaatca agcatttagc taacctgaat tagttcatgc
     1861 cgtatttttt caatttttta cgcagcgtac cacggttgat gcccatcatc agcgcagcac
     1921 gggtctggtt accacgggtg tattgcatca ccatgtccaa caggggctgt tctacttcag
     1981 ccagtaccag ctcatagagg tcattcacat cctgaccatt cagttgagca aaatagttct
     2041 tcagtgcctg tttaaccgag tcacgcaggg gtttttgggt tacctgatcc tgagagttaa
     2101 cggtagaaac ggtcagtacg tcagaattta cgcgttgttc gaacatagtt ctgtcagctc
     2161 tttatttctg tttacgcaaa attttcgaag tatgcctcca acgcctccag ctgttcgctg
     2221 gcatcctcaa tggcgttgaa tgtgcgccga aactggtcat ttggagcgtg ttcctggaga
     2281 taccaggaaa cgtgtttacg tgcaattcgg tacccttttg ccggaccata aaagtcatgc
     2341 agttcccgaa cgtgcgcgca aagcaagcgc ttaacctctg ccaaaggcag cgggggcagc
     2401 aactccccag tgtccagata atgctggatt tcccgaaaga tccagggtct tccctgagct
     2461 gcgcggccta tcatcagggc atccgcccct gtatagtcga gcacagctct ggctttaagc
     2521 gggtcagtaa tgtcgccatt cgcgataacc ggaatggaaa ctttctgctt aactgcccga
     2581 atactgtcgt actcagcttc tccattgaac aaacaggcgc gtgtacggcc atgaatggtc
     2641 agagcctgaa tgccacagtc ttcagccagt tgggcaatct cttcgcagtt acggtgttcc
     2701 ggtgcccagc cggtgcgaat cttcagggta acaggaacgt ccactgcatt gacgacctcg
     2761 gtaaggatcg atttaacgac atccgggtac tgcaagaggg ctgaacctgc gagcttgcga
     2821 ttcacttttt tagccgggca acccatattg atatcaataa tctgggcacc gctttccacg
     2881 ttaatacgtg ctgcatctgc catttctttc ggatcgctac cagcaatttg cacggtgcga
     2941 ataccgggtt catcaatgtg caccatccgt aaacgagatt tgtcgctttc ccaaacctgt
     3001 gggttagaag acatcatctc ggatactgtc aatccggctc ccatctcgta gcacaacgtc
     3061 cgaaaaggtc tgtctgtaat gccagccatg ggcgctgcga tcaggcgatt tctgagctga
     3121 tattgtccga tgcgcatgag ttaagaaatg accatactgt gactgcaagg cggcgtatat
     3181 tacgcatttt ttgcacgaga tgaaaggcca aactttgacc aatcctctga gatggatcaa
     3241 agaattgcat ttaaaatgag cgtggtgcga taattactca taaaaatcat catattagaa
     3301 aatagtgact aaaatttaca ctcaaagaaa tttgagtaag ttctcaattt ttctttatga
     3361 atgaaaattt tggcacgcaa atttgcgtaa ataatcggca aatttacgtg ccttttgtga
     3421 gcttgctcgc acttcgcccc gcgtcaccct acggcgatgc gaaggttaat tcttacgacc
     3481 ggtaatacgg caccactctt ctttttccac gaccgggtcc agtgcgaagc tgtcggcata
     3541 agcttcacaa acgctctctg cctggcttgc cagaataccg gaaaggccca gcaaaccgcc
     3601 tgaaaccggc aggacgctga ttaacggtgc cagttcacgt aatgggcctg caaggatgtt
     3661 agcgaccacc acgtcggctt tcatttcttc tggctgatct ttcggtaagt agagttccag
     3721 acggtcagaa acgccattac gttcggcgtt gtcgcggctg gcctgaatcg cctgcggatc
     3781 gatatcaata ccaatggctt ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc
     3841 ggaaccacag ccaaagtcga tgactgtttt accggttaaa tcgaggctgt cgagccattg
     3901 caggcacaga gaggtggttg gatgggtacc cgtaccaaac gccagccctg gatctaacat
     3961 cacgttgacg gcgttttcgt ccggcacatc acgccagcta gggcagatcc acagtcgttc
     4021 accaaagcgc atcgggtgga aattatccat ccattcgcgc tcccagtctt tatcttctag
     4081 ttgttcgatt ttatgcgcga agcctgcgcc gagcagcgga tggttttcca gaatcgccac
     4141 cacgtcgttc atatcggttt cagcgtcaaa cagaccaatc acatcggtgt cgccccacag
     4201 gcgcgtttcg cccggcagcg gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat
     4261 agaaacggca cccgcttcca tcagcgcatc gctaagatct tccgcgttcg cgccggtggt
     4321 gttcagtttc agttggatcc aaggcatggc aaaactcttt atttatcagt agtcaaaacg
     4381 gtagcttgcg ggacggatgt accgaaacgg tttccgacca ggaaagccag caaacttagt
     4441 agtaacgagg gcacgatagg gtggaagccc aggtactgaa tattcagcgt cgcgagtacg
     4501 gcatacagca cgccgccaac gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc
     4561 cagtaaagac ccagcaccag cggccacagg aaaacggctt ccagcccacc gaaggccagc
     4621 aaattcagcc agatgatcat ttctggcggc ttccaggcgg caagcagcag caacgcgccg
     4681 agaactaacg taattaccgc cgacatccgc ttcagacgcg tctcgttttg catttgatcc
     4741 ggacggatat tcagatagag atctttaatg atcgtagcgg aactttgcag caactgggca
     4801 ttgatcgtcg acatgatcgc agccataggt gcagccagga agattccggc agcaaacggt
     4861 ggcagcactt ttaccattaa cgtcgggatc accagatccg gtacggtgag atcggggatc
     4921 actgcacgac ctaacgctcc ggccaggtgc ataccgaaca tcaggattgc gactacaatc
     4981 gtaccgatga tgatcccccg gtgtactgct ttgctgtctt tataagagat acagcgcacc
     5041 gcagtatgcg gcaggccaat cacgccaaag cacaccagta cccagaacga cgtcataaag
     5101 gcaggcgaca gaatatcgtc agcgccttgt ggcgtaacca gttgcggatc gatggtttgc
     5161 aaggtctgta ctgcgttgct taagccgcca gcggcatgta ccacgccaat aagcagcaca
     5221 acggtgccaa tcagcatcac aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag
     5281 ccaccaaagg cggtatataa cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa
     5341 ggaatacccg ccgcggtttc cagcaggcgc gcaccgccga taaactgcac ggtcatcgca
     5401 ccaacgaacg caaccagcaa actcaaactc gccagccaca ccagaagacg actctggtag
     5461 cgggcaaaca gcatatcgtt cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac
     5521 ttcttgccga gaataccgag tgaaagccag actgcaggaa gctgaatcat cgccagcaat
     5581 acccagccca gcccgtattt ataagcagct cctggcccgc cgataaacga actggcactg
     5641 atataggtcg cggtgagcgt catcgccagc acaataccgc ccatagagcg gctgccgagg
     5701 aaatactcat taaggaaggt gccggtgctc cgtttacgca tcgcataaac cgagataccg
     5761 aacaccacca ccagataggc gaccagcggt agaattactt caagctgcat cgtcatcctc
     5821 cagtgggata tcgcgataga taaatttcac catcgcccag cacagtccaa taaacagcag
     5881 cggcgtcagg atgcaggcca tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac
     5941 gccaggtaag taagcggcta ctaaccatac cgccagatac aaaagggtca gccccagcgc
     6001 ccagcgcgcc tctttatggg cctgaacaaa acgagtgtcc attttttgtc cctgatgggt
     6061 gaagaaagcg aggattgtac cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg
     6121 aaaatccggc cttttgacgc ttttgcagtc ttatttttcc tgaagaccga gttttttctc
     6181 cagatagtgg atgttagtgc caccatgctg gaagttctcg tcattcatga tgcggatctg
     6241 cagatcaacg ttggttttga taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg
     6301 ggcaatcgcc acgtcacggt tttcaccgta gcaaatcagc ttaccgatca ttgagtcata
     6361 gtacggcggt acggtgtagc ccgcgtagat atgagactcc caacgcactc caaaaccgcc
     6421 aggtgcgtgg aaacgggtga ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc
     6481 gttgatacga cattccaccg catggccgcg aacgtgaact tcttcttgct tgatcgacag
     6541 cggttgaccg gcagcgatac gcagctgttc tttgatcagg tcaacgccgg tgatcatttc
     6601 agtaaccggg tgttctacct gaatacgggt gttcatttcg atgaaataga actcgccgtt
     6661 ttcgaacagg aactcgaaag tacctgcacc gcgatagccg atatcaacac acgctttagc
     6721 gcaacgttcg ccgatgtagc gacgcagttc cggggtaatg cccggtgctg gtgcttcttc
     6781 gaccactttc tggtggcggc gctgcatgga gcagtcacgt tccgccagat agatagcgtt
     6841 gccctgaccg tcagccagta cctgaatctc gacgtggcga ggattttcca ggtatttctc
     6901 catgtaaacc atatcgttgc tgaaagcagc tttcgcttcc gcacgggtca tggagatgga
     6961 ttgtgccagt tcagcgtcgc cgcgcactac gcgcataccg cgaccaccgc cgccgccgga
     7021 ggctttgata atcaccggat aaccaatgcg tttagcaatg gcacggtttt tatccatatc
     7081 gtcgcccagc gggccgtcag aacccggtac gcaagggacg cccgcttttt tcatcgccgc
     7141 gattgcggat actttgtcgc ccatcaggcg aatggtttct gctttcgggc caatgaagat
     7201 aaagccggag cgttcaacct gctcggcaaa gttggcgttc tcggagagga agccgtaacc
     7261 cggatggatt gctactgcgc cggtgatttc agcggcgctt atgattgccg ggatgttcag
     7321 ataacttttt actgacggag cagggccaat acagaccgtt tcatctgcca gtaatacgtg
     7381 ttttagatcg cgatccgcgc tggagtgcac agcgacagtc ttgatgccca gttctttaca
     7441 ggcacgaaga atacgcaatg caatctcgcc gcggttggca ataacaattt tatccagcat
     7501 gttcgcctcg ttactcgatg acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt
     7561 cgaccagaat tgctttcacg gtaccggatt tgtccgcttc gatctggttc atcattttca
     7621 tggcttcaac gatgcacagg gtatcgccca cgttgacttt ctgacccact tcgatgaacg
     7681 cttttgcgtc cgggcttggg gtgcggtaga aagtaccaac catcggggag cgtacgatgt
     7741 gaccactgat ttccgctgct gctggcgctt ccatggaagg aacggtcgcc ggagcggctg
     7801 cgttagattg agctggctgc tgcatcattg gtgcagcgta agcttgttgc atcacaggga
     7861 aacttgcggc aggagctgca cggctgatgc gtactgactc ttcgccttcg gagatttcca
     7921 gttcggagat gcctgattct tcaaccagct cgatcagttt tttaatctta cgaatatcca
     7981 tgagtgggtt ccgtactctt tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt
     8041 aaagcgtatg ccccgacagc tgtatgcata gcgataaatt ccagcaggcc gggacgccgg
     8101 tctattttgc cccagagtgt caatgttaga cttgacggac attgtgcagc gatctgctgc
     8161 tatcctccgg caaaaaacaa aatatacctt cgttgcgcca atgtcacctt ttccgcagcg
     8221 caaaaactgc gtcagggagg acgaggtcgc acattataac gatttcgtag caattggcag
     8281 ctaaatactg gtcttatcag ggaagataat caacagctaa catgtaaata accttcaaca
     8341 ccgtgtaatt tgcaacaagc cgcacaattc acgaaattag cgccatcatc gacggaactt
     8401 cttataacgt aaggtgaaaa gctgtaaagc cagccctgcg aagatgaggg gctgcaatga
     8461 gataatcttc acagaccaca aataatgtag ggatgccagg atcgcgacaa gatagacgac
     8521 attacgcagc ggttgctggg gattacccaa ttttcactgc atcgtctgga tagatgtgaa
     8581 tgctaaagca cgcaaaatca accgactgac aattctcagc attaaatagg ttcgagtggt
     8641 taatttgtag tcaaataaaa tcagattatt caccccatgg gaccagaaat cagtaacagg
     8701 ttaagcgtag tgtcgcccag gcaaagcacc ataaacctaa caggcgacgg aggcatatca
     8761 ataatggctg tttagaggag tatcaatgcg catcaatttg tggtcagtcg aaatcatgac
     8821 tttccagttt ggatgatgtt gtacaacttc ctgaccaagg tgttcgacga aagctgtccg
     8881 gctaaccgct tttcccgtta accgattgaa taatgatgtc aaaatgagtt ccctctctta
     8941 ttattccttg ctaaatggtt agttaacctt caccagcgtg cgaccctgga tctggttatt
     9001 aatgatggcc tcggcaaagt tcggtgcctc tgacagagat atctcttttg ccgcctgggt
     9061 atagaatgat tccggtaaat cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg
     9121 tggcgtcatt actgaatcca ccccttgcaa acggacatta cgcagaataa atggcatgac
     9181 cgtggttggc agagtaaaac cacccgccag accacaggcc gccacgcagc cgccgtaatt
     9241 catttgcgcc agcacttttg ccagcacttt gtcgccaaca gtgtcaattg ccccagccca
     9301 gacttgtttt tccagaggac gggattcggc aaactcatca cgagggagaa tacgactggc
     9361 acctaaactt ttcagatatt catgggtact ttcgcgaccg gaaacggcaa cgacctgata
     9421 acccaactta tgcagcagcg ccacggcggt actgccgacg ccaccactgg cacccgtcac
     9481 gacaatctcc ccgtcctgcg ggcgaacacc ggcatcttcc agcgccatca cacacagcat
     9541 ggcggtaaaa ccggcagtac cgataatcat tgctttacgc gcgtccagcc cttgcggcat
     9601 ggcaaccagc cagtcacctt tcactcgcgc ctgctccgcc agcccacccc agtggttttc
     9661 accaacgccc cagccagtga gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct
     9721 ggtgcgtaca gttccggcaa aatcgatccc aggaatcatc ggaaaattac ggatgatttt
     9781 tcccttaccg gtaatcgcca gcgcatcttt atagttcagg ctcgaccagt gaacatcaac
     9841 cgtgacatcg ccctccggca ggcgactttc gtcaagtgtc tgtaccgatg cgagagtttt
     9901 gccgtcctgc tgttctaaaa gtaacgcctg cataagtggt cctcatgtgc atgatggatt
     9961 ggaaaataat ttctgaagac tatactcgct aatggaaaag caaagccgat gaagcgcaat
    10021 aaattgcgag ataaatctga tttgctagta tgcccgcttc ctcactatcg gagttaacac
    10081 aaggatgaga ttaacgacga aattttcggc ctttgttacg ctgctcaccg ggttaacaat
    10141 ttttgtgact ttgctgggct gttcgctaag tttctacaac gcaattcagt ataagtttag
    10201 tcatcgcgtt caggcggtgg cgacggcgat tgatacccac cttgtgtcga atgacttcag
    10261 cacattaagg ccacaaatta ccgaattaat gatgtcggca gatatcgttc gtgtagacct
    10321 gctccatggt gataaacagg tttataccct ggccagaaat ggtagttatc gtccggttgg
    10381 ctccagcgat ctgtttcgcg aactgagtgt tccgttgata aagcatccgg ggatgtcgct
    10441 gcgtctggtt tatcaggatc cgatgggcaa ctatttccat tctttaatga ctaccgcgcc
    10501 gctcacgggg gcgattggct ttatcattgt tatgctcttt ctggcggtac gctggttaca
    10561 acggcaactt gccgggcaag aattgctgga aacccgggct actcgtatct taaacggtga
    10621 gcgtggctct aatgtgttgg gaaccatcta tgaatggccg cccagaacca gcagtgcgct
    10681 agatacgctg cttcgtgaaa ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct
    10741 gatccgctct tatgccgccc aggacgtgaa aaccggcctc aataaccgac tctttttcga
    10801 taatcagtta gcaacgttac tggaagatca ggagaaagta ggtacccacg ggatcgtgat
    10861 gatgattcgt ctgccggatt tcaatatgtt gagcgatact tgggggcaca gccaggttga
    10921 agaacagttc ttcactctga cgaatctgct gtcgacattt atgatgcgct accctggcgc
    10981 actgctggcg cgttaccacc gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa
    11041 agaggcagag agcatcgccg gtcagttaat caaagccgtc gataccttgc cgaacaataa
    11101 aatgctcgat cgcgacgata tgatccacat tggtatctgc gcctggcgta gtggtcagga
    11161 taccgagcag gtaatggaac atgcagagtc tgccacgcgt aatgcgggat tgcagggcgg
    11221 caatagctgg gctatttacg atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg
    11281 gcgtacgctt atcgagcaaa tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc
    11341 ggttactcgc gaaggtcagg ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa
    11401 tgaagaggtt agctcggcgg agtatatgcc gatggtcttg cagtttggct tatcggaaga
    11461 gtatgaccgt ctgcaaatca gccgtcttat tccactattg cgttactggc cagaggaaaa
    11521 tctggcgatt caggttaccg ttgagtcgct gattcgcccg cgttttcagc gttggctgcg
    11581 cgatacgtta atgcaatgtg aaaaatcaca acgaaaacgc ataattattg aacttgcaga
    11641 ggccgatgta ggtcaacata tcagtcgttt acaacctgtt attcgtttag tgaatgcttt
    11701 aggggtacgg gtagccgtca accaggctgg tttgacgctg gtaagcacca gttggatcaa
    11761 agaacttaat gttgagttac tcaagctcca tccggggctg gtcagaaaca ttgagaagcg
    11821 aacggagaac cagctgctgg ttcaaagcct ggtggaagcc tgctccggga ccagcaccca
    11881 agtttacgcc accggtgtgc gttcgcgaag cgagtggcag accctgattc agcgcggtgt
    11941 tacaggcggg caaggggatt ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa
    12001 atattcacaa agatactcgg tttaacctgc cgtttaatcc gttttcacgt agaataacgc
    12061 gcgctgcgtc tcatgggagt gtgcttgtct gctcgccaga ttgttgcagc acatatgcag
    12121 atgaatgacc ttacgcggtt gcaaacaggc gaggaatgct gctgatgcat taagcctttc
    12181 tggactcagg cagagatttg taacaaagga aacgaactgc actaattttc accgtagcag
    12241 atgatttttg cgccttgtcg ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc
    12301 cgccccagct ttcaggatta tcccttagta tgttgaaaaa atttcgtggc atgttttcca
    12361 atgacttgtc cattgacctg ggtactgcga ataccctcat ttatgtaaaa ggacaaggca
    12421 tcgtattgaa tgagccttcc gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa
    12481 gcgtagctgc agtaggtcat gacgcgaagc agatgctggg ccgtacgccg ggcaatattg
    12541 ctgccattcg cccaatgaaa gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc
    12601 tccagcactt catcaaacaa gtgcacagca acagctttat gcgtccaagc ccgcgcgttc
    12661 tggtttgtgt accggttggc gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc
    12721 agggcgctgg tgcccgtgaa gtcttcctga ttgaagaacc gatggctgcc gcaattggtg
    12781 ctggcctgcc ggtttctgaa gcgaccggtt ctatggtggt tgatatcggt ggtggtacca
    12841 ctgaagttgc tgttatctcc ttgaacggcg tggtttactc ctcttccgtg cgcattggtg
    12901 gtgatcgttt cgacgaagct atcatcaact atgtgcgtcg taattacggt tctctgatcg
    12961 gtgaagccac cgcagaacgt atcaagcacg aaatcggttc ggcttacccg ggcgatgaag
    13021 tccgtgaaat cgaagttcgt ggccgtaacc tggcagaagg tgttccacgc ggttttaccc
    13081 tgaactccaa cgaaatcctc gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg
    13141 taatggttgc actggaacag tgcccgccgg aactggcttc cgacatctcc gagcgcggca
    13201 tggtgctcac cggtggtggc gcactgctgc gtaaccttga ccgtttgtta atggaagaaa
    13261 ccggcattcc agtcgttgtt gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca
    13321 aagcgctgga aatgatcgac atgcacggcg gcgacctgtt cagcgaagag taatcggatg
    13381 caggcagggg aaatgtctgt ttaccctgcc tggtctgata cgagaatacg cataacttat
    13441 gaagccaatt tttagccgtg gcccgtcgct acagattcgc cttattctgg cggtgctggt
    13501 ggcgctcggc attattattg ccgacagccg cctggggacg ttcagtcaaa tccgtactta
    13561 tatggatacc gccgtcagtc ctttctactt tgtttccaat gctcctcgtg aattgctgga
    13621 tggcgtatcg cagacgctgg cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg
    13681 tcaggaactg ttgctgaaaa acagtgaact gctgatgctt ggacaataca aacaggagaa
    13741 cgcgcgtctg cgcgagctgc tgggttcccc gctgcgtcag gatgagcaga aaatggtgac
    13801 tcaggttatc tccacggtta acgatcctta tagcgatcaa gttgttatcg ataaaggtag
    13861 cgttaatggc gtttatgaag gccagccggt catcagcgac aaaggtgttg ttggtcaggt
    13921 ggtggccgtc gctaaactga ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct
    13981 gccaatccag gtgctgcgca acgatatccg cgtaattgca gccggtaacg gttgtacgga
    14041 tgatttgcag cttgagcatc tgccagcgaa tacggatatt cgtgttggtg atgtgctggt
    14101 gacctccggt ctgggcggtc gtttcccgga aggctatccg gtcgcggttg tctcttccgt
    14161 aaaactcgat acccagcgcg cttatactgt gattcaggcg cgtccgactg caggactgca
    14221 acgtttgcgt tatctgctgc tgctgtgggg cgcggatcgt aacggcgcta acccgatgac
    14281 gccggaagag gtgcatcgtg ttgctaatga acgtctgatg cagatgatgc cgcaggtatt
    14341 gccttcgcca gacgcgatgg ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc
    14401 gactccgcag caaccgacga caggaaatgc agctactgcg cctgctgtgc ccacacagcc
    14461 tgctgctaat cgctctccac aaagggctac gccgccgcaa agtggtgctc aaccgcctgc
    14521 gcgtgcgccg ggagggcaat agtggcgagc tatcgtagcc agggatgttg ggtaatctgg
    14581 ctctctttcc tcattgcgct gttgctgcaa atcatgccct ggccggataa cctgattgtt
    14641 ttccggccaa actgggtgtt actcatcttg ttgtattgga tcctggcctt gcctcatcgc
    14701 gtaaatgtgg gcacaggttt tgtgatgggt gccatactgg atctgatcag cggctcgacg
    14761 cttggcgtac gcgtattggc gatgagcatc attgcttacc tggtggcgct gaaataccag
    14821 cttttccgca acctcgcatt atggcagcag gcgctggtcg tcatgttgct ttcgctggtg
    14881 gtggatatta ttgttttctg ggcagagttt ttagtgatta acgtctcttt cagaccggaa
    14941 gtgttctgga gtagtgtagt caatggggtg ctctggccgt ggattttctt gctgatgcgt
    15001 aaagtccgtc agcagtttgc agtgcaataa aggtttctat gacttctctg tatttagctt
    15061 ccggttctcc gcgtcgtcag gagttacttg cgcaacttgg cgtgaccttt gaacgtattg
    15121 ttacgggcat tgaggagcag cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc
    15181 tggcgcgcga gaaagcacag gcgggtgtcg cgcaaacggc gcaggatctc ccggtgctgg
    15241 gtgcggatac tatcgttatc ctgaatggag aagtgctgga gaaaccgcgc gacgcagagc
    15301 atgcggcgca gatgttgcgc aaattatcgg gtcagaccca tcaggtgatg acggcagtgg
    15361 cgttggccga cagccagcac attctcgatt gcctggtggt caccgatgtg actttcagaa
    15421 cgttaacaga cgaagacatc gcgggctatg tcgccagcgg tgaaccgtta gataaagcag
    15481 gtgcatacgg tattcagggg ctgggtggct gttttgtcag gaagataaat ggcagctatc
    15541 acgccgtagt cggcttaccg ctggttgaaa cgtatgaatt attaagtaat tttaacgcac
    15601 tgcgtgagaa aagggataaa catgacggct gaattgttag taaacgtaac gccttcggaa
    15661 acgcgagtgg cgtatattga tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg
    15721 cgacgcggaa tagtaggcaa tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg
    15781 caggcggctt ttgtagatat tgggctggat aaagccgcgt ttcttcatgc atctgacatc
    15841 atgccgcaca ccgaatgtgt ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc
    15901 tcggaactgg ttcgtcaggg gcaagatctg atggtgcagg tggtgaaaga tccgcttggc
    15961 actaaaggtg cgcgcctgac caccgatatc acgctgcctt ctcgctatct ggtgtttatg
    16021 ccaggggctt ctcacgttgg ggtttcccaa cgtattgaaa gcgaatcaga acgtgaacgc
    16081 ctgaaaaaag tggtcgcaga gtattgcgac gagcagggcg ggtttatcat ccgtaccgca
    16141 gcggaagggg ttggcgaggc tgaactggcc tccgatgccg cttatctgaa acgcgtctgg
    16201 accaaagtta tggagcgtaa aaaacgcccg cagacccgtt atcagctgta cggcgaactg
    16261 gcgctggcgc agcgtgttct gcgtgatttc gccgacgccg aactggaccg cattcgcgtt
    16321 gactcacgcc tgacttacga agcattgctg gagtttacct cggagtacat tcccgagatg
    16381 accagcaagc tggagcatta caccggacgc cagccgattt tcgatctctt tgatgtcgaa
    16441 aacgaaatcc agcgagcgct ggaacgcaaa gtagaactga aatccggtgg ttatctgatt
    16501 atcgaccaga ccgaagcgat gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat
    16561 cgcaatctgg acgacaccat tttcaatacc aatattgaag cgacgcaggc tatcgctcgc
    16621 cagttacggt tgcgtaatct gggtgggatt atcattattg atttcatcga tatgaataat
    16681 gaagatcacc gccgccgcgt gctgcactcg ctggagcagg cgttgagcaa agaccgggtg
    16741 aaaaccagcg ttaatggttt ttcggcgctg gggctggtgg agatgacgcg taaacgcacc
    16801 cgcgaaagca ttgagcacgt actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg
    16861 gtgaaaaccg tggaaacggt atgctatgaa atcatgcgcg agattgttcg tgtccaccat
    16921 gcttacgact ccgaccgttt cctggtctat gcatctccgg cagtagctga agccttgaaa
    16981 ggcgaagagt cacactcgct ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta
    17041 caaattgaac cgctctataa ccaggagcag tttgacgtcg taatgatgta aacagatgct
    17101 gggccgccat ccggcaaagg gtttttgagt cacattttta gcagacaagg agtgacgggt
    17161 gaggcgattg ccggggattt tactgcttac tggagccgcg ctcgttgtga tcgctgctct
    17221 gctggttagc ggcctgcgta ttgctttacc gcatcttgac gcctggcgtc cggaaatcct
    17281 caacaaaata gaatccgcga ctggcatgcc ggtagaagcc agtcagctct cagccagctg
    17341 gcagaatttt ggcccgacgc ttgaagcaca cgacatccgt gcagaactaa aagatggcgg
    17401 cgaattttcg gttaaacgcg ttactctggc gctggatgtc tggcagagcc tgttacatat
    17461 gcgctggcag tttcgcgacc tcactttctg gcagctgcgc tttcgcacca acactcctat
    17521 caccagcggt ggtagcgatg acagtctgga agccagtcac atcagcgatc tgtttcttcg
    17581 tcaatttgac catttcgatc tccgcgacag tgaagtcagt tttctgacgc catccggtca
    17641 gcgcgccgag ctggcgatcc cacaactcac ctggctgaac gatccacgtc gacaccgtgc
    17701 ggaaggcctg gtaagcctct ccagccttac cggacagcac ggcgtgatgc aggtgcgcat
    17761 ggatttgcgc gatgatgagg ggttgttaag caatggtcgc gtctggctac aggcggatga
    17821 catcgacctg aagccgtggc tcggtaaatg gatgcaggac aatattgctc tggaaacggc
    17881 acagttctcc cttgaaggct ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt
    17941 ctggctgaaa cagggcggtg ccagctggtt gggcgagaag caaacgcata cgctgtcggt
    18001 ggataatctg accgcgcata ttacgcgtga aaatccgggc tggcagttct ctattcccga
    18061 tacacggatc acgatggacg gcaaaccctg gccgagcgga gcattgacgc tggcctggat
    18121 accggaacag gacgttggcg gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc
    18181 cagtaatctg gagctggcag gcctggaggg catacgcccg ctggccgcga aactttcacc
    18241 tgcactgggt gatgtttggc gctccacaca accgagcggc aagattaaca ctctggcgct
    18301 ggatatcccg cttcaggcgg cagacaagac ccgttttcag gcatcgtgga gcgatctggc
    18361 ctggaagcaa tggaaattat taccgggtgc ggaacacttc tccgggacgc tttccggcag
    18421 cgttgaaaat ggtttgctta ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt
    18481 attccgtgcg ccactggaaa tcgccgacgg ccaggcaact ataagctggc tgaacaatga
    18541 caaaggtttc cagctggatg ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg
    18601 cggcggtttt cgttacctgc aacctgctaa cgatgaaccc tggctgggta ttctggctgg
    18661 catcagtacc gatgatggtt cacaagcctg gcgctatttc ccggaaaact tgatgggtaa
    18721 agacctggtt gattacttaa gtggcgcgat tcagggcggt gaagcggata acgcgacgct
    18781 ggtttatggt ggcaatccgc aactcttccc ctataaacac aacgaaggtc agtttgaagt
    18841 gctggtgccg ctgcgcaacg cgaagtttgc cttccagccg gactggcctg cattaactaa
    18901 ccttgatatt gaactggact ttattaacga cggtttatgg atgaaaaccg atggcgttaa
    18961 tctgggcggc gtgcgcgcga gtaatctcac cgcagtgatc cctgactact cgaaagaaaa
    19021 actgctgatt gacgctgaca ttaaaggtcc gggtaaagcc gttggccctt actttgatga
    19081 gacaccgctg aaagattctc tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt
    19141 gaatgctcgc ttacatcttg atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga
    19201 agtgacgctg cgtaataaca gtctgtttat caaaccactc gacagcaccc tgaaaaattt
    19261 gagcggtaaa ttcagcttta tcaatggcga tctgcaaagt gaaccactga cagcaagctg
    19321 gtttaatcag ccgttgaacg tggatttttc caccaaagaa ggggcaaaag cctaccaggt
    19381 tgcggtgaat ctcaacggta actggcaacc ggcgaaaacc agcgttctgc ctgaagcggt
    19441 gaacgaagca ttgagtggca gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta
    19501 tcatgctggc gcgacctata acgtagagat gaacggcgat ctgaataatg tgagtagtca
    19561 cttaccttca ccgttagcca aacctgcggg tgaaccacta gcggtaaacg ttaaggttga
    19621 tggcaatctc aacagctttg aattaaccgg acaggctggt gcggataacc atttcaatag
    19681 ccgctggttg cttggtcaaa agctgacgct cgatcgtgct atttgggcgg cagacagtaa
    19741 aacgctcccg ccgttgccgg aacaaagtgg cgttgaactc aatatgccgc cgatgaatgg
    19801 tgccgagtgg ctggccctgt tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag
    19861 tttcccacaa cacataacgt tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa
    19921 taacctgagt attgtttcgc aaccgacggc aaatggcacc ctggttgagg cgcaagggcg
    19981 tgaaatcaac gccacgctag cgatgcgtaa taacgcgccg tggctggcga atatcaaata
    20041 tctttattac aacccgagcg tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt
    20101 cccgacaacg gagcgcatta acttccgtgg ctggccggac gcacaaatac gatgcacaga
    20161 gtgctggttc tgggggcaaa aattcggtcg cattgacagt gatatcacca tttctggcga
    20221 tacgttaacg ctgaccaatg gactgattga tactggtttc tcgcggctta ctgccgatgg
    20281 tgaatgggtt aataatccgg agaatgaacg tacctcgctg aaaggaaaac tgcgcgggca
    20341 gaaaattgat gccgccgcag aattttttgg tgtcacgacg cccatacgcc agtcgtcatt
    20401 taatgtggat tacgatttac actggcgcaa agcaccctgg caaccagatg aggcgacgtt
    20461 gaatggcatc attcatactc aactgggtaa aggcgacatt accgaaatca ataccggaca
    20521 tgccgggcaa ttgctgcgct tattgagcgt tgatgccctg atgcgtaagc tgcgttttga
    20581 tttcagagac acttttggcg aagggttcta ttttgactcc attcgcagca ccgcgtggat
    20641 taaagacggg gttatgcaca ccgacgacac gctggtggat ggcctggagg cggatatcgc
    20701 catgaaaggg tcggtaaatc tggtacgtcg cgacctgaat atggaagcgg ttgtcgcacc
    20761 agagatttct gcgacggtgg gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc
    20821 ggcagtattt gcagccagta aagtgctggg gccgctgtgg agcaaagtct ccattttgcg
    20881 ctatcacatt tcaggtccgc tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg
    20941 taaagaaaaa gcgcaatgat ttgacgaggg cgcgtaattg ccccaatctc atatgataat
    21001 cgttgccaaa ggccaacgag ccggaacata actgtaggcc ggatagggcg ttcacgccgc
    21061 atccggcagc cgtaaaaaat cctctactgc agtaactaac gagtagcaaa aacgatgagt
    21121 ctgaacctgg taagtgaaca attgctagcg gcgaacggcc tgaaacatca ggacttgttc
    21181 gcgatcctcg gtcaactggc cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg
    21241 agctatcacg aatcctgggt tttagaagac cgcattatta aagatggttc ttacaacatc
    21301 gatcagggcg ttggtgtgcg tgcaatcagt ggtgaaaaaa ccggatttgc ttacgctgac
    21361 caaatcagcc tgctggcgct ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat
    21421 agtggtgatg gcaaagtaca gacgctgggc gcggtagagc atagcccgtt gtatacctcg
    21481 gtagatccgc tgcaaagcat gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat
    21541 aaggttgccc gcgaagcgga caagcgcgta caagaagtga ctgccagcct cagcggcgtt
    21601 tatgaattaa ttctggttgc ggccaccgac ggcacgctgg cggcggatgt tcgtccgctg
    21661 gtgcgtcttt ccgtgagcgt tctggtcgaa gaagatggca aacgcgaacg cggtgccagt
    21721 ggcggcggcg gtcgttttgg ttatgagttc ttccttgccg atcttgacgg cgaagtccgt
    21781 gcggatgcat gggcaaaaga agcagtgcgt atggcgctgg tcaatctttc tgccgttgct
    21841 gcaccagcgg gcaccatgcc ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat
    21901 gaagcggtag gtcacggtct ggaaggcgac ttcaaccgcc gcggcacttc agtatttagt
    21961 gggcaggtcg gggagctggt ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg
    22021 gtcgatcgcc gtggttcggt ggcgattgat gacgaaggta cgccaggcca gtacaacgtg
    22081 ctgattgaga acggcattct gaaaggctac atgcaggata aactcaacgc gcgtttgatg
    22141 gggatgacgc cgactggcaa cggtcgccgt gaatcctacg cccatctgcc catgccgcgt
    22201 atgaccaaca cctatatgct gccgggtaaa tcgaccccgc aggaaattat tgaatccgtt
    22261 gagtacggta tctatgcacc gaactttggt ggcggtcagg tggatattac atccggcaaa
    22321 ttcgttttct ccacttcaga agcgtatctg attgaaaacg gtaaagtaac gaagccggtg
    22381 aaaggcgcga cgttgattgg ttccggtatc gaaaccatgc agcagatttc gatggttggc
    22441 aacgacctga aactggataa cggcgtgggt gtctgcggta aagaagggca aagtttgccg
    22501 gttggcgtgg gccagccaac gctgaaagtt gataacctga ctgttagcgg tactgcgtaa
    22561 taatttcatt attttcagaa ggataattaa tctttctacg tgcacgaacg gtcccctcgc
    22621 tcctctgggg ttagggtgag gggaacccgt tggcacaggt ttgtagaacg taacagtaca
    22681 atatgaatta cttctctttc ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa
    22741 caaaataatc cgtcagcgag ttgatcacga cctgtacctt cagcggcagc ttatcttttt
    22801 cggtatataa cgcataaacc gggcgtggat ctgactgata acgcggcagc aggatctcca
    22861 gctcaccgcg attgatctcg ttgatcaccc acatcagcgg tacgtaggcg atcccggcac
    22921 ccgccgttag ccagcgcacc agcgtcatcg gatcattagt cacaaatctc ccttgcggga
    22981 tcaggcgagt cgagatccct tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc
    23041 tgtattcaag ccatgaatgg ctacttaaat cggcgggttt ttccggtatg ccgtattgtg
    23101 tgagatagct tttcgcggcg cacaccacca ttggcatcgc gccaagacgg cgggaaaaca
    23161 ggctggaatc ctgcaacgcg ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt
    23221 cgggggctgg aattccggta accagattga cgctcaaacc tgggtattct ttcagcattt
    23281 tggctgtcag cccggcgaga acattttgtg ccatagttga agaacagcca atgcgtagcg
    23341 tcccgatggg ggtgttattg aaggcataca gttgctcatg aacatcctgc acttcatgaa
    23401 gcatacgacg gcagccctgg tagtaaattc taccggcttc ggtcaggcca atgctgcgtg
    23461 tgctacggtt taacagcttt acctgcaact catcttccag ttttgacacc gtctgactga
    23521 tggacgaaac gctcatctgt agctgtctgg cggcggcggt aaaagagcca aattcaacta
    23581 ctttggcaaa caccgacatg cgttttagtc gttccattat tcactctgac ttaaaagtga
    23641 tttagatcac ataatataga taacagcata acagttacgc taatatatta aatatcaatc
    23701 tacagcaatg ttgctctcgc ccggctttcc atgcccttct ctgcggcgac agatgctgaa
    23761 aataataacg cctgctctct ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc
    23821 gtggtgtttg ggctgtcctt cccaccgata ttttttgaat tgcttttatc actggcgatt
    23881 ttctggctgg tgcgccgggt acttgtgcca acaggtatct acgactttgt ctggcatccg
    23941 gcgttgttca acaccgcgct ctattgctgc ttgttttatt tgatatcgcg actgttcgtt
    24001 tgaggttgaa gtgaaaacac taataagaaa attctcccgt acggccatca cggtcgtatt
    24061 agtcattctg gccttcatcg caatttttaa tgcctgggtc tattacaccg aatccccctg
    24121 gacgcgtgac gcgcgcttta gcgctgacgt cgttgcgatc gcgccggacg tttctggact
    24181 cattacccag gtgaatgttc atgataacca gctggtgaaa aaaggacaga tactgttcac
    24241 catcgaccag ccgcgctatc aaaaggcgct tgaggaagcg caagccgatg ttgcttatta
    24301 tcaggtactg gcacaggaga aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca
    24361 ggcgatgtct cgcgaagaga tcgaccaggc taacaacgta ctacaaacgg ttctgcatca
    24421 gttagcgaaa gcgcaggcga cccgcgatct ggcaaaactg gatcttgaac gcacggtgat
    24481 ccgcgcgcca gcagatggct gggtgaccaa cctcaacgtc tataccggtg agtttattac
    24541 tcgaggatca acggcggttg cgctggtgaa acagaactcc ttctatgtac tggcctatat
    24601 ggaagaaact aagctggaag gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg
    24661 cagtaacaaa gtgctgaaag ggactgttga tagtgttgcc gctggggtca ccaacgccag
    24721 cagcacgcgt gacgacaaag ggatggcgac catcgactct aaccttgaat gggtgcgtct
    24781 tgcgcaacgt gttccggttc gtattcgtct cgacaaccag caagagaaca tctggcctgc
    24841 gggcaccact gctacagtgg tggtcactgg caaacaagat cgcgacgaaa gccaggattc
    24901 gttcttccgt aaaatggccc atcgcctgcg tgagtttggt taatcacgat gggtattttc
    24961 tccattgcta accaacatat tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg
    25021 ctgtttgttg gctttcactt ccagctggaa acgccacgct gggcggtact gacagcggcg
    25081 attgttgccg ccggtccggc ctttgctgcg ggaggtgaac cgtattctgg cgctattcgt
    25141 tatcgtggct ttttgcgcat catcggcaca tttattggct gtattgccgg actggtgatc
    25201 atcattgcga tgatccgcgc accattattg atgattctgg tgtgctgtat ctgggccggt
    25261 ttttgtacct ggatatcctc gctggtacga atagaaaact cgtacgcgtg ggggctggcc
    25321 ggttataccg cgctgatcat tgtgatcacc attcagccgg aaccattgct tacgccgcag
    25381 tttgccgtcg aacgttgtag cgagatcgtt atcggtattg tgtgtgcgat tatggcggat
    25441 ttgctctttt ctccgcgatc gatcaaacaa gaagtggatc gagagctgga aagtttgctg
    25501 gtcgcgcaat atcaattaat gcaactctgt atcaagcatg gcgatggtga agttgtcgat
    25561 aaagcctggg gcgacctggt gcgacgcacc acggcgctac aaggcatgcg cagcaacctg
    25621 aatatggaat cttcccgctg ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta
    25681 tcgctgacgc tgattaccca atcctgcgaa acttatctta ttcagaatac gcgcccggaa
    25741 ttgatcactg atactttccg cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc
    25801 cacaagcagc tcaaacgcct gcggagagtt atcgcctgga ccggggaacg ggaaacgcct
    25861 gtcaccattt atagctgggt cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt
    25921 atcagtaaca caaaaatcaa cgccaccgaa gaagagatcc tgcaaggcga accggaagtc
    25981 aaagtagagt cagccgaacg tcatcatgcg atggttaact tctggcgaac cacactttcc
    26041 tgcattctgg gaacgctttt ctggctgtgg acgggctgga cttccggcag tggtgcaatg
    26101 gtgatgattg cggtcgtgac gtcactggca atgcgtttgc cgaatccacg catggtggcg
    26161 atcgacttta tctacgggac gctggccgcg ctgccgttag ggctgctcta ctttttggtg
    26221 attatcccta atacccaaca gagcatgttg ctgctgtgta ttagcctggc agtgctggga
    26281 ttcttcctcg gaatagaagt acagaaacgg cgactgggct cgatgggggc gctggccagc
    26341 accataaata ttatcgtgct ggataacccg atgactttcc atttcagtca gtttctcgac
    26401 agcgcattag ggcaaatcgt cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg
    26461 gataaatcgc gcgacaggac tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt
    26521 tccgcgatga ctaccaatgt ggcacgtcgt aaagagaacc acctcccggc actttatcag
    26581 cagctgtttt tgctgatgaa taagttccca ggggatttgc cgaaatttcg cctggcgctg
    26641 acgatgatta tcgcgcacca gcgcctgcgt gatgcgccga tcccggttaa cgaggattta
    26701 tcggcgtttc accgacaaat gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat
    26761 gataaacgtc gtcggtactt tggtcagttg ctggaagaac tggaaatcta ccaggaaaag
    26821 ctacgcatct ggcaagcgcc accgcaggtg acggaaccgg ttcatcggct ggcggggatg
    26881 ctccataagt atcaacatgc gttgaccgat agttaagtca aaaccgacgc caaaagcgtc
    26941 ggttttttca tggctatact tagcgatgaa cggcagaact cgccgcgaaa cgtgacggtg
    27001 gcaacagatg aatatttata cctttgattt tgatgagatt gagagtcagg aggattttta
    27061 tcgcgacttc agccaagcct ttggtctggc gaaacataag gtacgcgatc tcgactcact
    27121 atgggatgtg ttaatgaacg atgtcctgcc gctaccactt gagattgaat ttgttcatct
    27181 gggagagaaa acgcgtcgcc gttttggcgc gttaatattg ctgtttgatg aggcagagga
    27241 agagctggaa gggcatttgc gttttaatgt tcgtcattag cgcaaaaaaa agcccccgaa
    27301 ccgggggcaa tatcgtcgga caagacgatg agggtttatt tgtacagttc agccgtagcg
    27361 tgccaggtgt caccgctacg agcttcagta atctggtagg ccgttgcgcc tttctcttcc
    27421 gcttttttgt tcagcatttc acgtatatcc attggcgaag acgccacacc acttacggat
    27481 acggtcccga ttgcttcacg gttttgtgct tgtgcagcat caatggagtc ggcagcgaat
    27541 gcaccgaaag agagaacaga aagtacgctt aatgcagcaa cagtggtttt gattttcatg
    27601 atttttacct cgacataatc tttttgctgg gtctttgttt cgtgaccctt atcacaaaat
    27661 caagtataca ctaattattg aactaattaa taccacgcta actgtttctg ttgatactaa
    27721 gtgttaaaaa tgtgaatgtt aataacaaaa aagaattaaa aatacggtta tttatcgtta
    27781 attctagtgt tttcaattag ataatgaatt ttgcataatg tgcttttttt accttatgta
    27841 ttcattgtgt gaatgacatg tcgcagtaaa acgcactatt cgttaaatta atcgcagggg
    27901 aaagcagggg gttgagaggg ataagcaaca ttttccccgc cgccagaagc gacggggcag
    27961 agattaaagc tcctggtcga acagctctaa aatcgcttcg tacaggtctt tgacggtgaa
    28021 accgttagca ggggtggtaa agatggtgtc atcgccagcg atggtgccca gaataccttc
    28081 tgctttgccc agtgagtcca gcaggcgagc aattaactgc gccgcgccag ggctggtatg
    28141 aatcacgaca actgcatcgt tgtagtcgat atccagcacc agatttttca atggactgga
    28201 ggtggttggt acacccagtt cagctggcag gcagtaaacc atttccattt tggcattgcg
    28261 tgtacgtaca gcaccaaact tggtcaacat ccgcgagact ttagactgat taatattgtc
    28321 aaagccttgc tcctgcaacg cggcgacgat ttcgccctgg gagctaaatt tctcttcttt
    28381 aagtaatgct ttaaatgctt taactagttc ttcttgctta gccgagcttc gcataagtca
    28441 cccgatatgg tggttgatac aacattattg tgcatacaga tgaattttta tgcaaacagt
    28501 cagccctgaa gaaggctgaa ataatgttat gaaagagcgg gattttatca aatttcgtta
    28561 ttgagaaaca tgcctgcgtc acggcatgca aattctgctt aaaagtaaat taattgttat
    28621 caaattgatg ttgttttggc tgaacggtag ggtatattgt caccacctgt tggaatgttg
    28681 cgctaatgca taagcgactg ttaattacgt aagttaggtt cctgattacg gcaattaaat
    28741 gcataaacgc taaacttgcg tgactacaca ttcttgagat gtggtcattg taaacggcaa
    28801 ttttgtggat taaggtcgcg gcagcggagc aacatatctt agtttatcaa tataataagg
    28861 agtttaggat gaaagtcgca gtcctcggcg ctgctggcgg tattggccag gcgcttgcac
    28921 tactgttaaa aacccaactg ccttcaggtt cagaactctc tctgtatgat atcgctccag
    28981 tgactcccgg tgtggctgtc gatctgagcc atatccctac tgctgtgaaa atcaaaggtt
    29041 tttctggtga agatgcgact ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag
    29101 gtgtagcgcg taaaccgggt atggatcgtt ccgacctgtt taacgttaac gccggcatcg
    29161 tgaaaaacct ggtacagcaa gttgcgaaaa cctgcccgaa agcgtgcatt ggtattatca
    29221 ctaacccggt taacaccaca gttgcgattg ctgctgaagt gctgaaaaaa gccggtgttt
    29281 atgacaaaaa caaactgttc ggcgttacca cgctggatat cattcgttcc aatacttttg
    29341 ttgcggaact gaaaggcaaa cagccaggcg aagttgaagt gccggttatt ggtggtcact
    29401 ctggtgttac cattctgccg ctgctgtcac aggttcctgg cgttagtttt accgagcagg
    29461 aagtggctga tctgaccaaa cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag
    29521 ccggtggcgg gtctgcaacc ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc
    29581 tggttcgtgc actgcagggc gaacaaagcg ttgtcgaatg tgcctacgtt gaaggcgacg
    29641 gtcagtacgc ccgtttcttc tctcaaccgc tgctgctggg taaaaacggc gtggaagagc
    29701 gtaaatctat cggtaccctg agcgcatttg aacagagcgc actggaaggt atgctggata
    29761 cgctgaagaa agatatcgcc ctgggcgaag agttcgttaa taagtaatta attagcgaat
    29821 aataaaaaac cggagcacag actccggttt tttgttttga gcactcgact taattggttg
    29881 ccggatattc ctgaatggtg acctgcagcg ttaactgctt atcatcacgc atcactacaa
    29941 ccgggatcac cgaaccaggg cgaatttctg ccacctgatc catcgtctcc agagcagaga
    30001 tggccggttt gttatccacc gaaataatca gatcgttgac ctgaataccc gcattcgccg
    30061 ccgggccgtc aggtgacact tcattaacca cgatcccttg cagttgatct ataccaccgc
    30121 cctgcgcgtg cagtggtgcg atctcgcgtc caccgatacc aatgtagccg cggatcacgc
    30181 gaccatcgcg gatcagctta tccataattt tggttgctaa ctggaaagga atcgcaaagc
    30241 cgataccttc cggcgtttcg ccatcgttac tcttatcaaa cgacagcgtg ttaatgccca
    30301 tcagttctcc cagcgagttc accagcgcgc cgccagagtt accgtggtta atggaagcat
    30361 cggtttgcag gaagttttgc cgcccggtcg ggttcagacc gattcgaccc gtagcactaa
    30421 taatcccctg ggtaatggtc tgcccgaggt tgtacgggtt accgatcgcc agtaccacgt
    30481 cgccaatgtg cggtacgcga cgtgcattga tcggaatggt aggtaaaccg ccagtggcat
    30541 taattttaag taccgccaga tcggttagag agtcagatcc caccagcaat gcttcaaata
    30601 cacgtccatc ctgtaaggcg acgatgatct gatcggcgtc gttgatgacg tgtttattgg
    30661 tgatgatata accgcgttga tccatgatta caccggatcc cagggtgcgg atctcaagct
    30721 ggttgtgaga gttggtgttc aaaccacggt tgtaaacgtt aaccaccgct ggcgcggcgc
    30781 ggcgaaccgc cagattataa ctggcaggcg tctcatcggt actgtcaaat tgcggagtgg
    30841 aaagcgggtt aaggctgcgc agcgaaggca tggcaaccag cagaatagcg ccgacaatta
    30901 atccaatcgc aacggaacgt aagagcttca caaacatgat ggaggcgtca ttaaaaaagg
    30961 gaacggcagc agcataccac gagttaaccg gacatcacac gtaagcctga tgcccggttt
    31021 acgacattaa cgcatcagca gatagatgct ttcattaccg cgtacaattt gcagggcgat
    31081 gatggccggt tttgccgcca gcactttacg catttcagca atcgagttca cccgatcgcg
    31141 gttgacgcca atgatcacat cgtctttttg caagccagcc tgagcagctg ggcttccttt
    31201 gacaacttca tcgatcttaa tacctttgcc gccatctttt agctgaccat cgctcaacgt
    31261 tgcaccttcc agcgctggcg tgatcatttc agcgctggcc gacgaagagg tgctggtatc
    31321 aagcgtcact tctacttcca gtggtttgcc gttacgcagc aggccaagct tcactttcgt
    31381 gcccggctct gtagtcgcga tacgagagcg caactcagca aagctattca gcggtttgcc
    31441 gttgaggctg gtaataatat cgcccgcttt gacgcccgct tttgccgagc cagaacctgg
    31501 caacacttcg ctgacaaacg cgccacgctg cacgtcaagg ttgaaggctt tggcgatatc
    31561 ggcactcatc tcggtgcctt tgatgcctaa caaaccgcgt ttgatttcac caaagtcgat
    31621 aagctgctgc gccagtgttc gcgccatatt actggggatg gcaaatccaa tcccgacgct
    31681 cccgccgcca ggcgcaagga ttgcagtgtt gatgccaatt aactcaccgt taaggtttaa
    31741 tagtgcaccg ccggagttac cgcggttaat ggaagcatct gtctggataa agttttccag
    31801 accttcaaga ttcaacccgc tgcggcctaa tgcggaaaca atgccagagg tggcggtttg
    31861 cccaaggcca aatgggttac cgaccgctac ggcaaaatca ccgacgcgca atttatcgga
    31921 gtcggcaata gcgatttgcg ttaatttgct cgggttttga atttgtaaca gggcgatatc
    31981 gctctggtca tcgctaccaa tcagttttgc atcaaactcg cgcccatcat tgagctgaat
    32041 actgattttc tgtgcctgat taatcacatg gttgttggtc agcacatagc ctttactggc
    32101 gttgatgatg acaccggaac ctaaaccttc gaagggttgt gcaggttgat ccggtaaatc
    32161 atcaccaaaa aactttttga attcttccgg gattttctgt ccctgactgg ccgttccttc
    32221 cacccgtacg ctcaccactg ccggaagcac tttttccagc attggagcca gactggggag
    32281 aggggcctga tcggcaacct ggcctggaat cgacgcgacg gcctgaaatg acgccgagag
    32341 agttaacccg acacttaacg ctaatgcact caacagctgg gtttgttttt tcattattcc
    32401 tgctctcgta cctgaatgat aagaaaagag attcaaaaca gtttgatgtt attgaatttt
    32461 cagacggcta acaatgagac accagattaa atgatagtcg gggagggaga gaagaggagg
    32521 gcgcaatggc tgcgcccgaa aaataaatta gtcgcgcttc gcgccagtac gcagcaggcc
    32581 ggatgcgcct tcagaatagt cgcgaggcat ctgcaccggt gcctgatcgt tgctggcttc
    32641 agactctgcc agacgattac ggaacgggtt tgcttcagca gacagttccg gcagcaggct
    32701 gctggagctt ttcgccatgt gctgatacag ctggcgatag tcgtgcgcca tggtatccag
    32761 taattccgcg ctgcgggcaa agtggctaac cagctcttcg cgatactcgt ccagttcagc
    32821 tttattcttt tccagttcgt actgcaacgc ctgttgctgg cgtagtttac gattaccaaa
    32881 acgcatggcc acagcaccaa taatgatgcc gacgactaac ccaattagcg catattccca
    32941 ggtcatgaac atctcccgtt gtcttttgtt tccgtagggt gttggcttca ggctccatgc
    33001 ctgcggctga ttatgccact ataaccgtta attccacaga agtggaatcc cgactgcata
    33061 tcgcgtagtg tagaacggcc tttttttcgt caaccgtgaa caacggcgca ccgattattc
    33121 aaggaataac aataagatca tgcaaagcgt taccccaaca tcgcaatacc tgaaggcgct
    33181 caatgaaggc agccatcaac ccgacgacgt tcaaaaagag gccgtcagcc gcctggaaat
    33241 tatttatcaa gaactcatca atagcaggcc accagccccc aggacgagtg ggctaatggc
    33301 gcgggtcggt aagctgtggg gtaaacgcga agacacaaag catacgccag tgcgtggctt
    33361 atatatgtgg ggcggtgtag gacgcgggaa aacctggctg atggaccttt tctatcaaag
    33421 cctgccggga gagcggaaac agcgcctgca ctttcaccgt tttatgctgc gggtgcacga
    33481 agagctaact gccttacagg ggcagaccga tccgctggaa attattgccg atcgctttaa
    33541 agccgaaact gacgtgctct gttttgacga attttttgtt tctgatatta ccgacgccat
    33601 gctacttggc ggtctgatga aagccctgtt cgcccgcggc attaccctgg tagcgacgtc
    33661 aaatattccg ccggatgaac tttatcgaaa tggcctgcaa cgtgcgcgtt ttctgcctgc
    33721 aatcgatgcc attaaacagc attgtgatgt aatgaacgtg gacgctggtg ttgattatcg
    33781 actgcgtaca ctcactcagg cgcatctgtg gctttcgcca ctcaacgatg aaacccgggc
    33841 gcagatggat aaactatggt tggcgctggc gggggcgaaa cgagaaaatt caccgacgtt
    33901 agaaatcaac catcggccat tggcgacaat gggcgtcgag aaccagacgc tggcggtctc
    33961 ttttactacg ctgtgcgtcg acgcccgcag tcagcatgac tatattgcgc tctcacgtct
    34021 ctttcatacg gtcatgttgt ttgatgtacc agttatgacg cggttgatgg agagcgaagc
    34081 gcggcgcttt attgcgctgg tggatgagtt ttacgagcgc catgtcaaat tagtggtgag
    34141 tgcagaagtg ccgctgtatg aaatttatca gggcgatcgg ctgaagtttg agttccagcg
    34201 ttgcctgtca cgtctgcaag agatgcaaag cgaagagtat ctgaagcgcg agcatttggc
    34261 gggttaaaac ctgtcacaaa tcacaaaaag gggtcgatct ttgaccccga cttctctata
    34321 atcctgcgac cccacgttac aagaaagttt ttttcccaaa actttttgtg tgctggcata
    34381 ggctattcga aggggtaggt ttgccggact ttgtcgtgtg aacctcaaca attgaagacg
    34441 tttgggtgtt caccaacgtg taactattta ttgggtaagc ttttaatgaa aacttttaca
    34501 gctaaaccag aaaccgtaaa acgcgactgg tatgttgttg acgcgaccgg taaaactctg
    34561 ggccgtctgg ctactgaact ggctcgtcgc ctgcgcggta agcacaaagc ggaatacact
    34621 ccgcacgtag ataccggtga ttacatcatc gttctgaacg ctgacaaagt tgctgtaacc
    34681 ggcaacaagc gtactgacaa agtgtactat caccacaccg gccacatcgg tggtatcaaa
    34741 caagcgacct ttgaagagat gattgctcgc cgtcctgagc gtgtgattga aatcgcggtt
    34801 aaaggcatgt tgccaaaagg cccgctgggt cgtgctatgt tccgtaaact gaaagtttac
    34861 gcgggtaacg agcacaacca cgcggcacag caaccgcaag ttcttgacat ctaatcggga
    34921 ttataggcaa tggctgaaaa tcaatactac ggcactggtc gccgcaaaag ttccgcagct
    34981 cgcgttttca tcaaaccggg caacggtaaa atcgtaatca accaacgttc tctggaacag
    35041 tacttcggtc gtgaaactgc tcgcatggta gttcgtcagc cgctggaact ggtcgacatg
    35101 gttgagaaac tggacctgta catcaccgtt aaaggtggtg gtatctctgg tcaggctggt
    35161 gcgatccgtc acggtatcac ccgcgctctg atggaatacg acgagtccct gcgttctgaa
    35221 ctgcgtaaag ctggcttcgt tactcgtgac gctcgtcagg ttgaacgtaa gaaagtcggt
    35281 ctgcgtaaag cacgtcgtcg tccgcagttc tccaaacgtt aattggcttc tgctccggca
    35341 gaaaacaatt tgcgaaaaaa cccgcttcgg cgggtttttt tatggttaaa ttctgaatca
    35401 gcgtaaaaat tggaaagttg ctttttgcta ccgtctgaca gacaggcaaa acaaaaacca
    35461 catcgccaat aagggactaa gtcaactatt tcagactaaa gcgcatctct ttttccccat
    35521 ttccggcatc gactcaccac aaaggtcgca aaatctggta aactatcatc caattttctg
    35581 cccaaatgtc gggtattgct cattttttgt ttgattttcg aacaaagaga gtcgttcctt
    35641 actgggtaac acaacttctg actggccacc tggtggctgg tagcagtaaa aattctgact
    35701 atacctggag gttttcatgg ctgtcgctgc caacaaacgt tcggtaatga cgctgttttc
    35761 cggtcctact gacatctata gccatcaggt ccgcattgtg ctggctgaga aaggtgtaag
    35821 tttcgagatc gaacacgtgg aaaaggacaa tccgcctcag gatctgattg acctcaaccc
    35881 gaatcagagc gttccgaccc tggtggatcg tgagctgacc ctgtgggaat ctcgcatcat
    35941 tatggaatat ctggatgagc gtttcccgca tccgccactg atgcctgttt acccggtagc
    36001 tcgcggtgaa agccgtctgt acatgcatcg catcgaaaaa gactggtaca cgctgatgaa
    36061 caccatcatc aacggttcag cttctgaagc agatgccgca cgtaagcaac tgcgcgaaga
    36121 actgctggcg attgcgccgg tcttcggtca gaagccgtac ttcctgagcg atgagttcag
    36181 cctggtcgat tgctatcttg ctccgctgct gtggcgtctg ccgcaactgg gcatcgagtt
    36241 cagcggcccg ggtgcgaaag agctgaaagg ctatatgacc cgcgtctttg agcgtgactc
    36301 tttccttgct tctttaactg aagcagaacg tgaaatgcgt ctgggccgga gttaatctgt
    36361 atggatttgt cacagctaac accacgtcgt ccctatctgc tgcgtgcatt ctatgagtgg
    36421 ttgctggata accagctcac gccgcacttg gtggtggatg tgacgctccc tggcgtgcag
    36481 gttcctatgg aatatgcgcg tgacgggcaa atcgtactca acattgcgcc gcgtgctgtc
    36541 ggcaatctgg aactggcgaa tgatgaggta cgctttaacg cgcgctttgg cggcattccg
    36601 cgtcaggttt ctgtgccgct ggctgccgtg ctggctatct acgcccgtga aaatggcgca
    36661 ggcacgatgt ttgagcctga agctgcctac gatgaagata ccagcatcat gaatgatgaa
    36721 gaggcatcgg cagacaacga aaccgttatg tcggttattg atggcgacaa gccagatcac
    36781 gatgatgaca ctcatcctga cgatgaacct ccgcagccac cacgcggtgg tcgaccggca
    36841 ttacgcgttg tgaagtaata caaaaacagg ccctggtggc ctgttttgtc ttttaatggc
    36901 ttaataaaat agcgtgattg tggcaatcat attcaccacg aaaccgactg ccatggggat
    36961 cgctgttcgc ttcaccaccg caaaaggcga aacgcctgca attcctgacg taacgaccac
    37021 caccgcagta atcggcgaaa ccgcgcgcgc tagcgtcgtg gcaaaatgca tcggcataat
    37081 cattacaacc gctggtacat gtagtccggc tgcgatattc ggaataagac tggcaaatga
    37141 cataaacggc gcattgccag agcccatcac aatggcacaa atggcaatga ctagcgccat
    37201 aataatcatc acgccaatac cgcccagacc agaatgctca gcacccctga taaccgcatc
    37261 aacagtgcca atcgtcgtta agcctttcgc aaatatttca cccgcgacca ccagcgttac
    37321 cacgttggca aactgcgtac ccatgccgtc aaaaaacgcc tgcacatcgt ccattgtttc
    37381 gcgcaagtta tgcttgcgga agaactcaac aaacatcgtc accgtcaaac tcagtaacat
    37441 caccaccacc agatgcagtt ctgactgcat cagcccgatg tgggcgagga acagcgagcc
    37501 gagcatcaag attaacggca tcacaggtaa aatggcgtaa tagagcggcg ggacattatc
    37561 gagagctttt agctctgcct gttcgtgatt gatattttta tcttttttgt caaaagcgcg
    37621 ttgcacgaaa aagtgggaga tcgccaccga gataatgacg caagaggcga ccggaagctg
    37681 gtagtggaag aagtaagtgg caattttcat tcccgcgacc tgggctgcaa aaatggagtt
    37741 cgtttccaga atcccccact caatggacat cgtggttgcg ataaccgcca ccgcagagag
    37801 gcgacttact cccagactca ccagcgtcgg aaataaggtg accatcagca acatacccag
    37861 gccggaggcg ctggtaataa actgcgccat gatttggccg atgacgtaag ttgccgacag
    37921 aataatatac ggcgagtgaa tgagttttaa cgggcggctt agcaagctca ccatcgcgcg
    37981 actggctcct gtgcgctcca tgtagcgggc ataaccgccc accgccataa tcgacagccc
    38041 caatccggca atgcggttgg acagcatgcg caggatttca ttataaatat cgacgatcaa
    38101 gtagccgctg cttttggcgg gatcgagtac accaccgaac cctaaccaga caccgcacat
    38161 catcaggaag atacctgcgg cagccagcac cacctgaggt ttgtagtttt tcaggatcaa
    38221 atagcccatc gtaattaata cgatgacaga tataattatg ccgaacattg ttgaactccg
    38281 tgtcaaaaga aaacggtcaa tcccataaac ggcagattga aaacaat
//
LOCUS       DAZGVQ010000028        37926 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000028 DAZGVQ010000000
VERSION     DAZGVQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37926
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.028"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(59..346)
                     /gene="yebO"
                     /locus_tag="ACHLAG_001402"
     CDS             complement(59..346)
                     /gene="yebO"
                     /locus_tag="ACHLAG_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFP0654662.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(421..564)
                     /gene="mgrB"
                     /locus_tag="ACHLAG_001403"
     CDS             complement(421..564)
                     /gene="mgrB"
                     /locus_tag="ACHLAG_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFP0654663.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            723..962
                     /gene="yobH"
                     /locus_tag="ACHLAG_001404"
     CDS             723..962
                     /gene="yobH"
                     /locus_tag="ACHLAG_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFP0654664.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1105..1896)
                     /gene="kdgR"
                     /locus_tag="ACHLAG_001405"
     CDS             complement(1105..1896)
                     /gene="kdgR"
                     /locus_tag="ACHLAG_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFP0654665.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2073..3446
                     /gene="yebQ"
                     /locus_tag="ACHLAG_001406"
     CDS             2073..3446
                     /gene="yebQ"
                     /locus_tag="ACHLAG_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0654666.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3492..4373)
                     /gene="htpX"
                     /locus_tag="ACHLAG_001407"
     CDS             complement(3492..4373)
                     /gene="htpX"
                     /locus_tag="ACHLAG_001407"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFP0654667.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4565..6613)
                     /gene="prc"
                     /locus_tag="ACHLAG_001408"
     CDS             complement(4565..6613)
                     /gene="prc"
                     /locus_tag="ACHLAG_001408"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFP0654668.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6633..7331)
                     /gene="proQ"
                     /locus_tag="ACHLAG_001409"
     CDS             complement(6633..7331)
                     /gene="proQ"
                     /locus_tag="ACHLAG_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFP0654669.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7428..7925)
                     /gene="msrC"
                     /locus_tag="ACHLAG_001410"
     CDS             complement(7428..7925)
                     /gene="msrC"
                     /locus_tag="ACHLAG_001410"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFP0654670.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8055..9338
                     /gene="yebS"
                     /locus_tag="ACHLAG_001411"
     CDS             8055..9338
                     /gene="yebS"
                     /locus_tag="ACHLAG_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFP0654671.1"
                     /translation="MALNTPQITPTKKITVRSIGEEQPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9307..11940
                     /gene="yebT"
                     /locus_tag="ACHLAG_001412"
     CDS             9307..11940
                     /gene="yebT"
                     /locus_tag="ACHLAG_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFP0654672.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12020..13459
                     /gene="rsmF"
                     /locus_tag="ACHLAG_001413"
     CDS             12020..13459
                     /gene="rsmF"
                     /locus_tag="ACHLAG_001413"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFP0654673.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13577..13813
                     /gene="yebV"
                     /locus_tag="ACHLAG_001414"
     CDS             13577..13813
                     /gene="yebV"
                     /locus_tag="ACHLAG_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFP0654674.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13918..14109
                     /gene="yebW"
                     /locus_tag="ACHLAG_001415"
     CDS             13918..14109
                     /gene="yebW"
                     /locus_tag="ACHLAG_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFP0654675.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14110..14766)
                     /gene="pphA"
                     /locus_tag="ACHLAG_001416"
     CDS             complement(14110..14766)
                     /gene="pphA"
                     /locus_tag="ACHLAG_001416"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP0654676.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15162..15503)
                     /gene="yebY"
                     /locus_tag="ACHLAG_001417"
     CDS             complement(15162..15503)
                     /gene="yebY"
                     /locus_tag="ACHLAG_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFP0654677.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15516..16388)
                     /gene="copD"
                     /locus_tag="ACHLAG_001418"
     CDS             complement(15516..16388)
                     /gene="copD"
                     /locus_tag="ACHLAG_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFP0654678.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLSVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNVSRLLFMLTTAQFILLAGVGHATMNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16392..16766)
                     /gene="yobA"
                     /locus_tag="ACHLAG_001419"
     CDS             complement(16392..16766)
                     /gene="yobA"
                     /locus_tag="ACHLAG_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFP0654679.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16905..17135
                     /gene="holE"
                     /locus_tag="ACHLAG_001420"
     CDS             16905..17135
                     /gene="holE"
                     /locus_tag="ACHLAG_001420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFP0654680.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17237..17893
                     /gene="yobB"
                     /locus_tag="ACHLAG_001421"
     CDS             17237..17893
                     /gene="yobB"
                     /locus_tag="ACHLAG_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFP0654681.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17917..18579
                     /gene="exoX"
                     /locus_tag="ACHLAG_001422"
     CDS             17917..18579
                     /gene="exoX"
                     /locus_tag="ACHLAG_001422"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFP0654682.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18576..20636)
                     /gene="ptrB"
                     /locus_tag="ACHLAG_001423"
     CDS             complement(18576..20636)
                     /gene="ptrB"
                     /locus_tag="ACHLAG_001423"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFP0654683.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASHDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFYAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20845..21504)
                     /gene="yebE"
                     /locus_tag="ACHLAG_001424"
     CDS             complement(20845..21504)
                     /gene="yebE"
                     /locus_tag="ACHLAG_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP0654684.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSMPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(21831..22187)
                     /gene="yebF"
                     /locus_tag="ACHLAG_001425"
     CDS             complement(21831..22187)
                     /gene="yebF"
                     /locus_tag="ACHLAG_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFP0654685.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22254..22544)
                     /gene="yebG"
                     /locus_tag="ACHLAG_001426"
     CDS             complement(22254..22544)
                     /gene="yebG"
                     /locus_tag="ACHLAG_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFP0654686.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22678..23856
                     /gene="purT"
                     /locus_tag="ACHLAG_001427"
     CDS             22678..23856
                     /gene="purT"
                     /locus_tag="ACHLAG_001427"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFP0654687.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23912..24553)
                     /gene="kdgA"
                     /locus_tag="ACHLAG_001428"
                     /gene_synonym="eda"
     CDS             complement(23912..24553)
                     /gene="kdgA"
                     /locus_tag="ACHLAG_001428"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP0654688.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24590..26401)
                     /gene="edd"
                     /locus_tag="ACHLAG_001429"
     CDS             complement(24590..26401)
                     /gene="edd"
                     /locus_tag="ACHLAG_001429"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFP0654689.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYVEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PRIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26636..28111)
                     /gene="zwf"
                     /locus_tag="ACHLAG_001430"
     CDS             complement(26636..28111)
                     /gene="zwf"
                     /locus_tag="ACHLAG_001430"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFP0654690.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28449..29318
                     /gene="hexR"
                     /locus_tag="ACHLAG_001431"
                     /gene_synonym="yebK"
     CDS             28449..29318
                     /gene="hexR"
                     /locus_tag="ACHLAG_001431"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFP0654691.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANSTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29446..30888
                     /gene="pyk"
                     /locus_tag="ACHLAG_001432"
     CDS             29446..30888
                     /gene="pyk"
                     /locus_tag="ACHLAG_001432"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFP0654692.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLKVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31019..31990)
                     /gene="lpxM"
                     /locus_tag="ACHLAG_001433"
                     /gene_synonym="msbB"
     CDS             complement(31019..31990)
                     /gene="lpxM"
                     /locus_tag="ACHLAG_001433"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFP0654693.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32110..33432)
                     /gene="mepM"
                     /locus_tag="ACHLAG_001434"
     CDS             complement(32110..33432)
                     /gene="mepM"
                     /locus_tag="ACHLAG_001434"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFP0654694.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33448..34380)
                     /gene="znuA"
                     /locus_tag="ACHLAG_001435"
     CDS             complement(33448..34380)
                     /gene="znuA"
                     /locus_tag="ACHLAG_001435"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFP0654695.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34459..35214
                     /gene="znuC"
                     /locus_tag="ACHLAG_001436"
     CDS             34459..35214
                     /gene="znuC"
                     /locus_tag="ACHLAG_001436"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFP0654696.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35211..35996
                     /gene="znuB"
                     /locus_tag="ACHLAG_001437"
     CDS             35211..35996
                     /gene="znuB"
                     /locus_tag="ACHLAG_001437"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFP0654697.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(36143..37153)
                     /gene="ruvB"
                     /locus_tag="ACHLAG_001438"
     CDS             complement(36143..37153)
                     /gene="ruvB"
                     /locus_tag="ACHLAG_001438"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFP0654698.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37162..37773)
                     /gene="ruvA"
                     /locus_tag="ACHLAG_001439"
     CDS             complement(37162..37773)
                     /gene="ruvA"
                     /locus_tag="ACHLAG_001439"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFP0654699.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         9096 a         9795 c         9351 g         9684 t
ORIGIN      
        1 taatcaggat gaattgagta acccttcaac gacggggtta ctcaagattg aggaaggatt
       61 atcgttcggc gaccagacga ataatatctt cgtcttcaac cgatttttga ctctcaacgg
      121 tctttttatc tgctttctca ggttcgtttg cttcgcacaa acgacgtaac agtgcatttt
      181 gacgtttttg ctgatccagc aacgcctcaa gcagttcaat ctgttcgtta gtccgagaac
      241 tggcacgatt gataaaaaac cacaagatga gcccgataag aagaaccacc accgatacaa
      301 ccaaagacgc aatgttcatc acgcctgaat ttacaacttc gttcatttca ccacctctga
      361 gtagaggcgc tattctacca ctgctggaga ggaagaaaat ctagtgctga aaaaatgata
      421 tcaccacggg ataaactggt taatggcaca aattccgctg aaaaattgta catcctgatc
      481 gcacatcatg ttgaatacct gcgcccaaag cagcaagcaa gccaacacca cgacaaccag
      541 aacgacccat cgaaactttt tcactccaat ctccgcttca cctaccttat gtcattaaac
      601 tagcatgcgt tagttaaaca gcgcctaact atgtcgatat tcgtgctttt tcggaatgag
      661 tagcttgctt cagcgcatat tgccgctacg attaagcgaa cataaaaaga gaagaggttg
      721 taatgcgatt catcattcgc acagttatgc tgatagcact ggtatggata ggattattac
      781 tcagcggata tggcgttttg attggaagca aagagaatgc cgcaggatta ggtttgcaat
      841 gtacctatct gactgccaga ggcaccagca cggtacaata cttgcacact aaaagtggtt
      901 tcttggggat aacagattgc ccactgttac gtaaaagcaa tatcgtggtc gataatggtt
      961 aagggtaata aataaccaga ttctcactca tgagtaaaat attcttatag caagcgcgtt
     1021 taatataacg caccttcaac tttatttttc tcatgctaaa taaagaacct gtctgtttga
     1081 tattccgaca ggttcttact ctcatcagaa cggatagtcg tgataaccca tttgggcaga
     1141 aattttgcgc gctgcggtat gcaacattgc gacatattcc tgtaaacgct cttcagagaa
     1201 acgcaacgtc gggaaggaga tgctcaaacc ggcaatgacc acgccaaagc gatcaaatac
     1261 cggtaccgca atgcatcgca gcccttcttc ctgctcttcg ttatcttcgc catacccctg
     1321 ctcgcgcacc tggtccagaa cgggtaataa cgcttctgta ctggtgatgg tccgctcggt
     1381 actgcgttta tactccacgc cctcaagaat ttgcttcact tcatcgagat cgcgccatgc
     1441 cagcagtacc ttaccaatcg cggtgctgta cagcggatta cgacgcccaa tccgtgaata
     1501 catgcgcaaa ttgtacatag agtcaatttt gtgaatgtaa acaatactgt cttcgtccag
     1561 tgcgccgagg tggatagttt ctttggtcag gcgggagatc tcacgcatct ggatatctgc
     1621 gctacgaatt aaatcgacgt tttgtaacgc gcgagcgccc agttcaaaca atttcagggt
     1681 cagggaatat ttctccgact ccccttcctg cgccacataa cctaaggttt tcatggtctg
     1741 taaaaagcga taaacggtgc tttttgacat catgacgcgc tgcgacagct cggttatccc
     1801 tatttcgcgc tcttcaccca gcgcctgcaa aatgccaaaa acttttagca cggaagatac
     1861 agaatcaggc tgtttatcca gatctgcgtt agccatttat cacctcattg cgagtgtttt
     1921 ataaaaatca gaactgtttt ttattataat ttcgcaccag ggtggtcgca atccatcttt
     1981 tgccggttag ttacaattct gcgacatcca ccgtgaatat cagtgctaga atcatacccc
     2041 tgttgattat tcaccaaaga tataaaattc ctatgccaaa agttcaggcc gacggcctgc
     2101 cattgcccca gcgatacggt gcgatattaa ccattgtgat tggtatttcg atggctgtcc
     2161 ttgacggcgc aatcgccaac gtcgccctgc caacaatcgc cacggacctt catgccacgc
     2221 cagccagttc catctgggta gtgaacgcct atcaaatcgc cattgtcatc tccctgctct
     2281 cgttttcgtt tctgggcgat atgtttggct atcgacgtat ttataaatgc ggtctggtcg
     2341 tttttctgtt gtcttcactg ttctgcgccc tttctgattc gctgcaaatg ctcacccttg
     2401 cgcgtgtcat acaaggtttc ggcggtgcag cgttgatgag cgttaatacc gcacttatcc
     2461 gcctgatcta tccacaacgt tttctgggga gagggatggg cataaactcg tttattgttg
     2521 ccgtctcttc tgctgccggg ccgacaattg ctgcagcaat cctctccatc gcatcctgga
     2581 aatggttatt tttaatcaac gtaccgttag gtattatcgc cctgcttctg gcgatgcgtt
     2641 ttctgccacc caatggttct cgcgccagta aaccccgttt cgacctgccc agcgccgtga
     2701 tgaacgcgtt aaccttcggc ctgcttatca ctgcgttgag tggtttcgct caggggcaat
     2761 cgctgacgtt aattgctgcg gaactggtgg taatggttgt tgttggtatt ttctttattc
     2821 gccgccagct ttctcttccc gtaccgctgc taccggtgga tttactgcgt atcccgctgt
     2881 tttcactttc tatttgcaca tctgtttgct ctttctgcgc acaaatgctg gcaatggttt
     2941 ccctgccctt ttacctgcaa accgtgctcg ggcgtagtga agtcgaaaca ggtttacttc
     3001 tgacaccgtg gccgttagca acgatggtga tggctccgtt ggcaggctat ttgattgaac
     3061 gcgtacatgc aggattgctg ggtgctctag ggttgttcat catggctgcg gggctttttt
     3121 ccctggttct gctgcccgcg tcacctgcgg atatcaatat tatctggccg atgatcttat
     3181 gtggcgctgg atttggcttg ttccagtcac ccaataacca caccattatt acctccgctc
     3241 ctcgcgaacg tagcggtgga gccagtggca tgttaggaac ggctcgtcta ctgggtcaga
     3301 gtagcggcgc ggcactggtg gcgctgatgc taaatcagtt cggtgataat ggtacgcacg
     3361 tttcgctgat ggctgcggct attctggcag tgattgctgc ctgtgtcagt ggtttacgta
     3421 tcactcagcc acgatccagg gcataaaaaa agcgcgtcga tcaggacgcg ctttttagta
     3481 tttacttcat attacttcag gtattcaccc gtacgcagag cttcaattcg tttatccagc
     3541 ggcgggtgag tcatgaacaa ctcactgagc gatttcgact taccgttaat gcagaaagcc
     3601 atcatgctgg ttgcttcttg cggttcatag ctggttttca ggcgctgcag cgcggcaatc
     3661 attttctcgc gaccaaccag ttttgccgaa ccggcatcag catggaattc acgatgacgc
     3721 gagaaccaca tggtgataat gctcgccaga ataccaaaca ccagttccag aaccgttgca
     3781 accgcaaagt agatcagcgg gttgccgttg ctctcttcac cttcatcacg atttccgccc
     3841 ataaaacccg cggcaagctg cgccagaata cgggaaataa agataacgaa ggtgttcacc
     3901 acgccctgaa tcagcgtcat ggtgaccata tcaccattgg cgatgtggct gatttcgtga
     3961 gcaattaccg cctcggcttc atccgggctc atgttctgca gcaaaccggt gctgacagca
     4021 accagagagg catcacggcg cgcaccggtt gcaaaagcgt tgatgtccgg cgcatggtag
     4081 atagccactt gcggcatagc gatccctgcc tgacgagcct gggttgctac agtattaacc
     4141 agccaacgtt ccctttcgtt acgcggttgc tcgatcactt ccccgccaac agatcgtaat
     4201 gccatccatt tggacatcag aagcgaaacg aaggaaccac caaaaccgaa cagcaaggcc
     4261 atgatcatca gcccctgaac gctgctcgac tgtatccctg tcaggctcag taccagcccg
     4321 aaaacgacca ttacggccag gttcgttagc aggaagagcg cgattcgcat cataattttc
     4381 tttttacctc agtttaacaa aacgcaatat gcgataccca catcgtatgg gttacgcgac
     4441 tattttcaag tctggatagt gcgtaagtca ccagaaagac acaactttac attttgtagc
     4501 atctgattta cggcatcttg ccgctgttaa aaaatcaggc acaatttctt gtgcctgatt
     4561 gatattactt gacgggagcg ggttgttccg cgggtctggc tttttcaagc ttcgccagat
     4621 cgagtgcgat attcaccgtc tcatccagat aaggatccgg ctcctggtaa tctttcggta
     4681 gatcatccag cttcttcaac tccggtttac cttcgcgttt aaagcgttcg ttcaaacgcg
     4741 ccagacgcgt cgcatcatct tcattattct ctttctcacg cacagcgtag ttcagagaaa
     4801 cgatattgcg cttgtccttc atagcgttga agcgcgcgat atccttcatg atgttctgga
     4861 actcaggatc tttcgcgata cgcgcattat gttccttcag cagctccggc ccaaaggccg
     4921 ttaaatcgcc tgatttcaca taagtcgcgg catcaatgct atcccacggc agcgcgttat
     4981 cttcgaattt ctcacccgtt tccgtttctt cattacccgt cggcatgatg atgtctggcg
     5041 ttacgccttt acgttgcgta ctgccgccgt taacgcgata gaatttctgg atcgtgtact
     5101 gcacagaacc cagcgctggc cattcaggac gtaacatctg atcgtaaata cggttcaatg
     5161 aacggtattg ctgaacggtg cctttaccaa acgtcggttc acccacaacc agcgcacgac
     5221 cgtaatcctg cattgccgcg gcaaagattt ctgaagccga agcactgaaa cggtcaacca
     5281 gtaccaccag cgggccttta tagaacacct gcccgtcggt atcgctgtct tcacgaacct
     5341 tgccgttgtt atcgcggacc tgaacaatgg gacccgcagg aataaacaga cctgagagcg
     5401 ataccgcttc ggtcaacgcc ccaccgccat tgctacgcag gtcgatgatc acactgctga
     5461 cattctgttt ttccagtttc tgcagttgaa ctttgacatc gtctgtcaaa cccacataga
     5521 agcccggaat atccagcacg ccgactttct ctttaccgac ggtcttcacc gacattttaa
     5581 ccgcgcggtc ttcgagacga atacgttcac gggtcaatgt tacagtgcgg gtcttggtcc
     5641 ctttaccagc aggtaaaatt tccagacgaa ctttactgcc cttcggccct ttaattaagg
     5701 caaccacatc atcaagacgc cagccaatca cgtcaaccat cggcttgcct gtttgaccaa
     5761 caccgacaat tttgtcacca acgctgatag ctttactctt cgctgccgga ccacctgcca
     5821 ccatcgaatt gataacggtg tagtcatcat ccatttgcag cactgcgcca ataccttcca
     5881 gcgacaaact catttcagtg ttgaactgtt cggtattacg cggggaaaga tagttggtat
     5941 gcgggtcgat ttcacgcgca aacgccgtca ttgccagcga gaaaacatct tcgctgttgg
     6001 tttgcgccag acgacgaatg gcaaatttgt agcggcgtgt cagggtttca cgaatttctt
     6061 tatccgtttt tcctgccagc ttcaggctta actcgtcgaa tttgacttta ctgtcccaca
     6121 gcgcgttcaa ctcagcctcg tttttcggcc agggcgcttt gctgcggtca aggttataag
     6181 tgtcgttgcc ggtgaaatcc atcggctttt ccagtaccga caaagcgtac tggtaacgct
     6241 caaaacggcg cttttgcgcc agattgtaga gatcgtagaa aacgtcgagt ttgcctgaac
     6301 gcagttcatc gcctaactcg gttttctttt tcgcgaactg ttcaacatcg cttgccagca
     6361 gcacgttgtg gctgtaatcg agcagattca ggtagcggtc aaagattttg gccgaaaatg
     6421 cctgatcgag gtcgaactgg cgataatgag aacgggtgaa gcgcgacgtt acgcgctcac
     6481 ttaccgtcgc atgctgcgtc tcttccttta ataccggaat ttgatcagca cgcgtgatat
     6541 cttctacagc gaaggtctgg cctgctattg caagcaggcc agctaacgcg gtaagcctaa
     6601 aaaacatgtt catgcctggc ccggcctccg tttcagaaca ccaggtgttc tgcgcgcaca
     6661 atcaaagaca tacccgaatt cagctggacg cggacgccgt ctttggtgat ttctaatacg
     6721 gtggcatcca tcgcgttttg acccgctttc accttcaggg cttgtccgac agtcagagct
     6781 gaaatgtcag aaaccggggt gtgctgttct tcgcgaggtg cttttactgt ttttggcgct
     6841 ttctctaccg gcttttgcgc acgaggttta cgttcagcgc cttctttgcg gcgtggcgtg
     6901 gtcggacgtg gcttgcgttc gcggcgcggt gcgtcttctt tctcaccagc agctgcggca
     6961 gcttcgcgtt ttttcgcttg ctgttcagca cgctgtgcct gaacacgcgc tttcgcttct
     7021 tcaagctgct tgcgagcatg ctctacatgt tgctcgtcca gctcaccgca tgggttgccg
     7081 tcaagatcga cacgcgttgc gccgggttta acaccgtaaa gataacgcca gctcgaagtg
     7141 tagagacgta aagcggatcg taattgcgtt ttgctcaggt tcatttcccc ggcaacacga
     7201 tcgaccaaat cctgaaaaat accgattttc agcggacgcg cttcaccttc cgcactgaaa
     7261 cagtggggaa aacgttcggc cagaaacgcg attacttctt tactgctatt caacttaggt
     7321 tgattttcca tgaaatttcc tgattacaac ggacgtagcc aacaagcgca ggcatgaaca
     7381 ggcgtcatta taatgacgct accagtaaat gctacgttat ccgttgatta tcctgcgacg
     7441 ctcgcaaaga attttttgta atccgtcgtt gcaagcactt tttcaagctg tgccacaagc
     7501 tgacgtaagc cttgctcgtc ctcgtctgta aagcgaccga agacggtact atcgatgtcg
     7561 agaacaccaa taatctgatt tttcaccacc agcggcagaa caatttcaga attactcgcc
     7621 gcatcacagg caatatgccc gtcaaacgcg tgaacatcct cgatacgctg cacttgattg
     7681 cgggcaaccg cagtgccgca caccccgcgc ccgacgggta tccggacaca ggcaattttg
     7741 ccctgaaatg gtccgagtac cagtgtatcg tcctcaagca aataaaaacc tgcccagttt
     7801 acgtcagtga gacgctcgta taacaacgca ctggtgttcg caagcgttgc cagaaaactg
     7861 gtttctcctg ccatcagcgc gttaaagtcg cgatttaaat ccgcgtaaaa ttctgttttg
     7921 ttcattatat aatcacttgg ttgtcttacc tggaactgcc agcctattaa aataagcatt
     7981 aaatgcgtta atgctcaaga tcattcccat catgggttaa tattaatgtt aattcttatt
     8041 acatttggca cgtcatggct cttaacacac cacaaattac gccgacaaaa aagataacag
     8101 tgaggtcaat cggcgaggaa cagccgcgtg gtgattacca acgttgcccg caatgcgaca
     8161 tgctgtttag cctgcccgag ataaattctc atcagagtgc ctattgtccg cgctgtcagg
     8221 caaaaattcg cgacgggcgc gactggtcgc taacgcgcct ggcggcaatg gctttcacca
     8281 tgctgttgct gatgccgttt gcctggggcg aaccgctgtt gcatatctgg ctgttaggta
     8341 ttcgtatcga cgccaacgtc atgcaaggca tctggcaaat gaccaaacag ggcgatacga
     8401 taacgggggc gatggtcttt ttctgcgtta ttggtgcccc cctcattctg gtgtcctcca
     8461 tagcatattt atggtttggt aaccgactgg gaatgaattt acgtccggta ctgctgatgc
     8521 ttgagcgact taaagagtgg gtaatgctgg atatctacct ggtcggcatt ggcgttgctt
     8581 ctataaaggt acaggattat gcccatatcc aggcgggtgt cggcttgttc tcttttgtgg
     8641 cgttggtgat tttaacgacg gtgacgttgt cacatcttaa tgtcgaggag ctgtgggagc
     8701 gattttatcc gcagcgcccc gctacgcgta gggacgagaa acttcgtgtc tgtcttgggt
     8761 gccattttac cggctatcct gatcagcgtg gtcgctgccc gcgttgccat atcccgctac
     8821 gcctgcgtcg ccgtcatagt ttgcaaaaat gctgggcggc gctgttagcg tcaatcgttt
     8881 tgttgttacc tgccaacctg ttgcctattt ctatcattta tctgaacgga ggacggcagg
     8941 aagatacaat tctttccgga attatgtcgc tggcaagtag caacattgcg gttgcgggaa
     9001 tcgtgtttat cgccagtatt ctggtaccgt ttactaaagt gatcgtcatg ttcactttac
     9061 tgttgagcat tcattttaaa tgccagcaag gtttacgcac acgcattctg ttactgcgga
     9121 tggtgacctg gattggtcgc tggtcgatgc tcgacctgtt tgtcatatct ttaaccatgt
     9181 cgctgattaa tcgcgatcag atcctcgctt ttactatggg accggctgcg ttttatttcg
     9241 gcgcagcggt aattttgact attcttgctg tggaatggct ggacagccgc ttactttggg
     9301 atgcacatga gtcaggaaac gcccgcttcg acgactgaag cgcagattaa aaataaacgc
     9361 cgtatctcac ctttctggct gctgccgttc atcgcgctaa tgattgccgg ttggctgatt
     9421 tgggacagtt atcaggaccg gggtaatacc gtcaccatcg actttatgtc ggcggatggt
     9481 attgtcccgg gccgtacgcc tgttcgttat cagggcgttg aagtcggaac agtgcaggat
     9541 atcagcctca gcgacgatct tcgtaagatt gaagtcaagg tcagcatcaa gtccgatatg
     9601 aaagatgcgc tgcgcgaaga gactcaattc tggctggtga cgccaaaagc atcgttggca
     9661 ggtgtctccg ggctggacgc cctcgtcggt gggaactata tcggcatgat gccgggtaaa
     9721 ggtaaagagc aggatcactt tgtcgcactc gatacccaac cgaaatatcg gctggacaat
     9781 ggcgatctga tgatccacct gcaagccccc gatctcggtt cgctgaacag cggttcattg
     9841 gtctatttcc gcaagatccc ggtgggaaaa gtctacgact atgccatcaa tcccaacaaa
     9901 caaggcgtgg tgattgatgt cctgatcgag cggcgtttta ccgatctggt gaaaaaaggt
     9961 agccgtttct ggaacgtttc cggcgttgat gccaatgtca gtatcagtgg cgcgaaggtg
    10021 aaactggaaa gcctggcggc actggttaac ggtgcgattg ccttcgattc accagaagag
    10081 tcgaaacctg ccgaggcgga agataccttt ggtctgtatg aagacctcgc ccatagccag
    10141 cgtggcgtaa taataaaact ggaactgccg agtggggccg gattaaccgc cgactcgacg
    10201 ccattaatgt atcagggact ggaagtcgga cagctaacta aactggattt aaatcctggt
    10261 ggtaaagtca ctggggaaat gacagttgat cccagcgtcg ttaccctgct tcgtgaaaat
    10321 acccgcatcg aattacgtaa tccgaaatta tcccttagcg atgccaatct cagcgccctg
    10381 ctgactggca aaaccttcga gttggtaccc ggcgatggcg agccacgcaa agagttcgtt
    10441 gttgtgccag gcgaaaaagc actgctgcat gaacctgatg ttctgacgct gaccctgacc
    10501 gcgccggaaa gttacggtat tgatgcgggt cagccgctca ttcttcacgg cgtgcaggta
    10561 ggccaggtta tcgatcgtaa actcaccagc aaaggcgtca cctttaccgt cgccatcgag
    10621 cctcagcatc gagaactggt aaaaggcgat agcaaatttg tcgtcaacag ccgtgtcgac
    10681 gtgaaggtgg ggctggatgg cgttgagttt ctcggtgcca gcgcctcaga atggattaac
    10741 ggcgggatac gtattctgcc gggcgataaa ggcgagatga aagccagcta tccactgtat
    10801 gccaatctgg aaaaagcgct ggagaacagc cttagcgatt tacccaccac aaccgtgagt
    10861 ttgagtgcag agacgctgcc ggatgtgcag gcaggatcgg tagtgctcta ccgtaaattt
    10921 gaagttggtg aagttattac cgtccgtccg cgagctaacg cgtttgatat cgatctgcat
    10981 attaagccgg agtatcgcaa ccttctgacc agcaatagcg tgttctgggc agaaggcggg
    11041 gcgaaagttc agctgaatgg tagtggcctg accgtacagg catccccgct ctccagagca
    11101 ttaaagggag ccattagctt cgataacctc agcggtgcca gcgccagtca gcgtaaaggc
    11161 gacaaacgaa ttctgtatgc ttccgaaaca gcggcccgtg cggttggtgg gcagattacg
    11221 cttcacgctt tcgatgccgg aaaactggcg gtcgggatgc caattcgcta tctcggtatt
    11281 gatatcgggc aaatccagac gctggatctg attaccgcgc gcaatgaagt acaggcaaag
    11341 gcggtgctct atccggagta tgtccagacc ttcgcccgcg gcggtacgcg cttctcggtg
    11401 gtcacaccgc aaatttcagc cgcgggcgtt gagcatctag atactatcct ccagccgtat
    11461 atcaacgtcg aaccaggccg gggcaatcct cgccgcgact ttgaattaca agaggccacc
    11521 attactgatt cgcgttacct ggatggctta agcattattg ttgaagcgcc ggaagccggt
    11581 tcgttaggta ttggtacgcc tgtgctgttc cgtggtctgg aagtcggtac ggttacagga
    11641 atgacgctgg ggacattgtc agatcgcgtg atgattgcga tgcgcatcag taaacgctat
    11701 caacacctgg tgcgtaacaa ttccgtcttc tggttggcat cgggttacag tctggacttt
    11761 ggtctgacgg gcggcgtagt gaaaaccggc acctttaacc agtttatccg tggcggcatc
    11821 gccttcgcca cgcctccggg tacgccactg gcaccgaaag cccaggaagg caaacacttc
    11881 ctgttgcagg aaagtgaacc gaaagagtgg cgtgaatggg gaactgcgct tcccaaataa
    11941 tgcccactgc tccggcgtgc ctgcgccgga gcgtttatgc taaactgcgc gcctgttttt
    12001 ttgccagtgg tacatgctcg tggcccaaca caccgtttat ttcccggacg cctttctgac
    12061 acaaatgcgc gaagcgatgc cttcgacgct ctcatttgat gattttcttg ccgcctgtca
    12121 gcgcccgttg cgccgcagca ttcgcgttaa tacgctgaaa atctccgttg ctgatttcct
    12181 gcaattaacc gctccttatg gctggacgct tacgccaatt ccgtggtgtg aagaaggttt
    12241 ctggattgaa cgcgacaatg aagatgcatt gccattgggt agtaccgccg agcatttaag
    12301 cggcctgttt tatattcagg aagccagttc aatgttgccc gttgccgcct tgtttgctga
    12361 cgataatgca ccacagcggg tgatggatgt cgcagctgcg ccaggctcca aaacgacgca
    12421 aattgccgcg cggatgaata acgaaggggc aatccttgcc aatgagtttt ccgccagtcg
    12481 ggtaaaagtg ttacatgcca atatcagccg ctgtggcatc agtaatgttg cgctcacaca
    12541 ttttgatggc cgcgtgtttg gtgcggcagt gccagaaatg ttcgatgcca ttttgctgga
    12601 cgctccctgc tctggcgaag gcgtggtgcg taaagatccc gatgcgctaa aaaactggtc
    12661 accagaaagc aatcaggaaa tcgcagctac acaacgggag cttatcgaca gcgcctttca
    12721 tgcattacgt cctggtggta cgctggttta ctcgacctgt accttaaacc aggaagaaaa
    12781 cgaagccgtt tgcctgtggc tgaaagagac ttaccccgac gcagtagagt ttttaccact
    12841 tggcgatctc ttccctggtg caaacaaagc gctgaccgaa gaaggctttt tgcatgtttt
    12901 cccacaaatt tacgactgcg aaggcttctt cgttgctcgt ctgcgtaaaa ctcaggcgat
    12961 tcccgcctta cccgccccca aatacaaagt gggcaatttc ccgttcagcc cggtgaaaga
    13021 tcgcgaagct ggacaaattc gtcaggcggc tgcaggtgtt ggcttaaact gggatgaaaa
    13081 cctgcgcctc tggcagcgtg acaaagaact gtggttgttc ccggtgggca ttgaagccct
    13141 gatcggtaaa gtccgatttt ctcggttggg gattaaactt gccgagacgc acaacaaagg
    13201 ttatcgctgg cagcatgaag cggttattgc tcttgcctca cccgataata tgaatgcatt
    13261 cgagctgaca ccgcaggaag cggaggagtg gtatcgcggg cgcgatgttt acccgcaagc
    13321 cgcgccagtg gcggatgatg tattggttac tttccagcat caaccgattg gtttagccaa
    13381 acggattggt tcgcgattga aaaacagcta tccgcgtgaa ctggtgcgcg atgggaaact
    13441 ttttaccggt aacgcctgac agcgcacaaa aaaagcgcac tttttgactg gcacattcgg
    13501 ctgcctcaac taggctgaaa aatggtgcga tcggactggt cgtaccacaa tcggcagcta
    13561 aatggagagc acgaagatga aaaccagtgt gcgcataggc gcttttgaaa tcgacgacgg
    13621 cgaattacac ggtgaatcgc caggtgatcg aacgttaacc attccttgta aatctgaccc
    13681 cgatttatgt atgcaactgg atgcctggga tgctgaaacc agtatccctg ccctgctaaa
    13741 tggcgaacac tctgtccttt accgtacccg ttacgatcaa caatctgatg cctggattat
    13801 gcgtcttgcc tgatccaaaa agaacccgtc ggcatggcgg gttatttgtc ctggttattc
    13861 ccccgttgta aaatctctcc taaacttaac ggtacggcac cacacttcgg ggatgaaatg
    13921 ttcgcgctgg tactttttgt ttgctacctg gatggcggtt gtgaagatat tgttgtggat
    13981 gtctacaaca cggaacagca gtgtctttat tctatgagcg atcaacggat ccgccatggc
    14041 ggttgttttc cgattgagga ttttatagat ggtttctggc gaccagcaca ggagtatggt
    14101 gatttttaat tattgcaatt gcacaagagt cagttcgccc ccaaagacag caccggtatc
    14161 aatataatgc aggttgccaa tatccacgcg atgtcgcaac ggtgtatgac caaaccagaa
    14221 atgatcagca cctgtaattc cctgcccttt ttggcgttca cctaatcgcg agcggctcca
    14281 caagacctga tgcaaatcaa cgtccttttg ccattcataa acatcatctg gataatcggc
    14341 atgagcaata acatgtttgc cggtacgact gtgtacttca agaataaagg gcaaatgctg
    14401 acatttttcc agcgccgttt tcgcttgttt ctgttgatta tctgccagcg caataaacca
    14461 gtcgccgcca ttcatcaacc acaaagacat ctgctgggat gccagcgcat ccatcgccat
    14521 ctgttcatga ttgcctctta ccgcacaaac ccaatgttgt tccagtaact gcagacaacg
    14581 taaactttgc ggcccacgat cgataacgtc tcctactgag ataagtaaat ctcgccacgg
    14641 atcaaaacga caatgccata atttgcggcg caactgctca agacaaccgt gtatatcgcc
    14701 agaaagccag atatgtcgcc attgatgacc cgcaattctc tgataaacgg gcgcaggctg
    14761 tttcatcaat attttcctcc cgcgctaaag atcacataat cttaacaaga atgttaaaaa
    14821 acgctggact cagacagtag agtgtgtgtt atggttgact ataaagtcag cgaaggaaat
    14881 gcttctggct tttaacagat aaaaagagac cgaacacgat tcctgtattc ggtccaggga
    14941 aatggctctt gggagagagc cgtgcgctaa aagttggcat taatgcaggc ttagttgcct
    15001 tgccctttaa gaatagatga cgacgccagg ttttccagtt tgcgtgcaaa atggtcaata
    15061 aaaagcgtgg tggtcatcag ctgaaatgtt aaaaaccgcc cgttctggtg aaagaactga
    15121 ggcggttttt ttattggaaa tcaaaaggct attttaggta attaacagag tttttcagct
    15181 cgttctataa acggtgccag actcattttt tcgccgggat tgttaggatc atcaatctga
    15241 atcaccgaaa tgggttgggc tttagtcttc ccactggcaa cttccttttg tgcgatatcg
    15301 tttaaaggat actgcacgag ggtacttggg ttaatgacat acaaagcatt acccggtctg
    15361 caagtcagca tcacctcttc gcgattaaac gcccatttgt ctttacccac ttcaaaacga
    15421 ctgacggtaa tcacctgcgg tgcagccagc gccgctgcag aactggtgag taacagaaac
    15481 gtcagaatac tttttttcat cattttcgta atccatcaaa aaggttcgag agtcgcaagc
    15541 aggctgatga tcaccagcac taccgcacct atcgcccatt cgagtttagt catccactca
    15601 aaccagggtg ccgcacgatc ttcatcttgc ctcatgcgcg gtacaagaac ataacgatta
    15661 gccagcgcaa tgaccaccat aatcatcacc aggatggctt tgagcaataa gagttggccc
    15721 cagtaagtcg taagcgtggg gggaaatccg gtaatcaaca atgcattaag cacgccgctt
    15781 gccagtacgc cgatcaccgc aaaatgcccg caccaggaaa aacgcatcag cgcctgaatg
    15841 gcctggtgtc gccaacggcc tttgatgagt tgcatacacc agagcaccgg cagtaatccc
    15901 ccaaaccacg ccgccgcaca aatcaggtga atcgcgtgat tagtctgatg gattttcgcc
    15961 gttacccctt cattcatcgt cgcatgcccc actcccgcca gcagaataaa ttgcgcggta
    16021 gtgagcataa acagcaaacg tgacacattc cgcggttgca taagggcgac gatcaacgtt
    16081 acgagggcga gaacaatttg ccataaccag acgccaccaa actgcgtttg caaaacagcc
    16141 tgccaaatgt ttggcgaaaa tacatctgcc catcccgttc ccatcaaccc gccctgaacg
    16201 gagagcattg cagtggcact gatcaaactc cagacggcag cgtgctgttg taaacgcaga
    16261 aaacgcttcg ccagcaagcg acgaatcgtt aagggggcca gccaggcacc gtacattgcg
    16321 aagccaaaga ccagcatcag cgaggtgaaa tggataaatc gcagcgcgat ccaggtaaac
    16381 gccagcatga tttatttcac gctaaaggtg tagtgtcctt tcgttttatg cccgtccacc
    16441 gaaacaacat gccagtcaac gatgtaggtt ccgggtttca gcgaatcggc cagtggaaca
    16501 atcagttgct tttggtcctg ctcatttcgc ttcgccggta atgttttaat attttcgttt
    16561 tttggccccg tgatttttgc accactgaat cctgtttcaa caccttccga gaagtttaaa
    16621 gtgattgcct gcggtgcagc tgtcacttgc gcgtttgccg caggatactg atgcgttaaa
    16681 tgcgcatgtg cccagacgga aggtgtcacc aacgaagtgg tcaggattgc cagcgcgtag
    16741 cgaagggagc gtgcagttga agtcatatta tctattcctt tttgtaataa cttttttaca
    16801 gagcataacc ttgtctaatg tctgagtcga ggatcatcaa ttccggcttg ccatcctggc
    16861 tcactcttag taacttttgc ccgcgaatga tgaggagatt aagaatgctg aagaatctgg
    16921 ctaaactgga tcaaacagaa atggataaag tgaatgtcga tttggcggcg gccggggtgg
    16981 catttaaaga acgctacaat atgccggtga tcgctgaagc ggttgaacgt gaacagcctg
    17041 aacatttgcg cagctggttt cgcgagcggc ttattgccca ccgtttggct tcggtcaatc
    17101 tgtcacgttt accttacgag cccaaactta aataaaactt atacagagtt acactttctt
    17161 acataacgcc tgctaaatta tgagtatttt ctaaaccgca cccataattt gcagtcattt
    17221 tgaaaaggaa gtcattatgt cgttctggaa agttgcagca gcgcaatatg agcctcgtaa
    17281 aacctcactc accgaacagg tagctcatca tctggagttt gtgagggccg ccgccaggca
    17341 acaatgtcag ctgttagttt ttccatcgct ttctttactg gggtgtgatt attcgcgacg
    17401 tgcccttcct gccccacccg atctctcact gttagacccg ctttgttatg ccgcaacgac
    17461 ctggcgaatg accatcattg ccggccttcc cgttgaatat aacgatcgct ttattcgtgg
    17521 aattgcggtg ttcgcaccgt ggcgaaaaac gcctgggatc taccatcaga gtcatggtgc
    17581 atgtctgggt cgtcgttcca gaaccatcac ggtggtcgat gaacaaccgc aaggtatgga
    17641 tatggacccg acctgttcac tgtttactac agggcaatgt ctgggagaac ccgacctgct
    17701 ggcgtcggcc cgtcgcttac aatttttttc acatcagtac tcgattgccg tgctgatggc
    17761 aaatgcccgt ggtaacagtg cactatggga tgaatatggt cgtctcatcg ttcgcgccga
    17821 tcgtggttca ttattgttag tcggtcagcg ttcatcacag ggttggcaag gcgatatcat
    17881 tccattacgc taggcttttt cggcctggag catgccatgt tgcgcattat cgatacagaa
    17941 acctgcggtt tgcagggagg gatcgttgag attgcctctg ttgatgtcat tgacggaaaa
    18001 atcgtcaacc ccatgagcca cctggtgcgc cccgatcgtc ctattagtcc acaagcgatg
    18061 gcgattcatc gcatcaccga agccatggtc gccgataaac cgtggattga agatgtgatc
    18121 ccacactatt acggtagtga atggtatgtc gcgcataacg ccagctttga ccgccgcgta
    18181 ctgcctgaga tgcccggtga gtggatttgc actatgaaac tggcccgtcg tttgtggcct
    18241 gggatcaagt acagcaatat ggcgttatat aaaacacgca agctcaatgt acagacgccg
    18301 ccgggcctgc atcatcaccg cgcgttgtat gactgttata tcaccgccgc gttgcttatc
    18361 gatattatga acacctccgg ctggacggca gaacagatgg ccgatatcac cggacgtccg
    18421 tcgttgatga cgaccttcac ctttggcaaa taccgtggca aagcggtttc cgacgttgcc
    18481 gaacgcgatc cgggctatct gcgctggtta tttaataacc tggacagcat gagcccggag
    18541 ctgcgtttaa cactgaaaca ttatctggaa aatacttagt ccgcaggcgt agcgggtaat
    18601 gttccctgcg ccagcgcgac cagaaaagca tattccatcg ctacgccttc gtacgattta
    18661 aagcgaccag atttaccgcc atggcctgag tccatgtcgg tacagagcaa taaaagatgg
    18721 tcatcggttt tcagctcgcg caatttagcg acccattttg ccggttccca atattgcacc
    18781 tgagaatcgt gcaaaccggt cgttaccagt aaatgcggat aagcctgtgc ggtgacgttg
    18841 tcatacgggc tgtagctttt catgtactcg taatattgcg gatcctgcgg gttaccccac
    18901 tcttcaaact caccagtggt aagaggaatt gattcatcaa gcatcgttgt tacaacatca
    18961 acaaacggta cctgggcgat aacgccgtgg aataattccg ggcgttgatt aattgcaacg
    19021 cccatcaaca tgccccccgc actcccgccc atcgcataac aaagcgaagg agagccatag
    19081 cccagtttta acaatgcatc gcaggcatca agataatcat taaacgtatt tttcttcttc
    19141 agaaattttc cgtcttcgta ccattgttgc cccagctcac caccgccgcg aacatggaca
    19201 atggcgtaga caaagccacg atctaacaaa ctcaagcggc taaaactgaa atcggcatca
    19261 atacttgcgc cgtaagaacc atagccatac accagcaacg ggttgtgtcc tttgcgaaaa
    19321 tgtttgcgat ggtagaccaa cgaaaccgga acttcgacgc catcacgggc gactatccac
    19381 aggtgttcac tgcggtaatt cgccgcataa aaaccaggaa cttccgtttg ttttaataca
    19441 cgacgctcac cggtatccat atccagttca aacaaagtgt ctggtgtagt catggaagaa
    19501 taaccataac gcaatcgcgc ggtttcaggt tctggattgt aggcaatcca ggtcacatag
    19561 gccggatcat caaaggcaat accaatgact tcccgggtct tgcggttaat ttggcgcaaa
    19621 ctggttaacc cgcgctgacg ctcttcaacc accagccagt cggtaaacag cgtaaaccct
    19681 tccagcatga tgttttcgcg tggcggaatt aactcttccc actgttgctc atcacgcata
    19741 cgggtacggt ataagccaaa gtttttgccg tggcggttgg aacgcagata aaaacgatgc
    19801 tggtagtgat caaggctgta ttcgtgatct ttgcggcgcg gcagaaaaac aaacggctcg
    19861 gcatcggcca tttccgcgtc cagcaggcga acttcactgg tggtggcgct ggccaaatga
    19921 atgactacat agtgcttcga ggtcgtttta tgcaggctga cgtaataggt atcgtctttt
    19981 tcttcgtaga tcagtttatc atgcgatgct ggcgtaccga tggcgtgacg ccagacctga
    20041 taaggcagca gcgtcaccgg atgcttgcga acatagtaga aagtccagga gtcatttgcc
    20101 cagacaaagc tgggttcaac gttatccagc agttccgggt accagttacc agtttccaga
    20161 ttacgaaaac gaatgccgta ctggcgtcgg gaaagaaaat cttctgccag cgccataatg
    20221 gtgttatcgg gcgtaatcgc cattccgccc atcgaataaa actcactatg agctgcgcgc
    20281 ttattggcat cgagcaatgt ttcccactca tcccactctt cactgaatgc cgattgacgc
    20341 tggtagatag catattcaca gcctggttca taaatatgcc gatagcggta gccatttttg
    20401 atgtagggcg cagaaacttc tcgttgcgga atgcggtcga tgatttcctt taagatgcga
    20461 tcctgcaagg cttgttgtga ggccatcacc cgatgaccgt aactattttc ttgttgcagg
    20521 tagtccagga cttctggctg agaacgcgta tcgtcccgca gccagtagta attatcgatg
    20581 cgcgtatcgc catgaagcgt catggcgtgg ggaatgcggg cggcttttgg tagcatgtta
    20641 ttgttctttc tggttgaaac atcttataag ggtggcaaaa ctcaccgggg atgcaagcga
    20701 aacaggggag tcattgctta gatgatgaca ggtaatggcg cggatatcga atgttatgca
    20761 gacagagaaa atcagcctgt tcaaatggct gtgcgattct ggatagcccg aaatagtcaa
    20821 cttcaggcta tccagagagc ggaattattc cgccaaagtg cgtttttgct gttcgagatc
    20881 gcgttcaatg ccgtcacgaa catcctgggg gattttcagc gcgtcaccca gtgcattcag
    20941 gtaactgcgt tccataaaat ggtcaatatc aatagccgcg caactcagga aatagatttc
    21001 cagcgcctct tcttcattgc ggaccccggt agccaggcgt tgtggatcca gcggttgttc
    21061 gattgcctgc tcaatgagta cacgcccctg ctcttccacg ccggcttcac gcaattgctg
    21121 gtcgattgcc gcacgttctt tggcatcaat atgaccatca cttttagcgg caaagaccag
    21181 cgcaaggatc aaacgttctg tacgctcatc cagcggcata ctttgcgcgc caaactgcgg
    21241 ttcgtcctga tgcgccgcgc gaattttatc tttgtattta ttccacagca ccgtacccgc
    21301 taccgctccg ccgccaacca gtaacgcgtt ggtgccatat ttcgtaagaa gtttacgtgc
    21361 tgatttattt gcgaccagca gcccagccag tccgcctaat gcgcctggca ctaacagttt
    21421 gcccaatccc tgatccgcag acgaggaggt agaagaactg ctttgcccaa gcagggattg
    21481 cagttgattt aaccagttag ccatatttgc tcctcaataa ccatttttag tgcctgtagc
    21541 atagcggatg aggatgtcag aaaatgtata tcgagacaaa agatgcgcaa ataagcgggg
    21601 cggtaggccg ttattcaaag aaattcgcgc caggtaaggt atttactgta tatcaggtga
    21661 taagggtatt ttaggtgaaa gtagtttgtc gttttgagtg gcattgtttg atgctgattg
    21721 cccgatgcga cgctgttgcg tcttctcagg cctacgattg tggtgcgttc tggggcggac
    21781 aaggcactcg cgccgcatcc gccaacggtc aatcgaatta tcagaagcca ttaacgccgc
    21841 tgatattccg ccattcccgc tttgcagtcc acgctgacct ggtaatgaat atcggcactt
    21901 ttaccacgca cggttagcgg tactgaccat ttatcatctt taccctgaat gtcctgcaaa
    21961 ctgacccaag ccacgggatc ggcctgaccg acaatttttt gatcatctgc ccagcgcgcc
    22021 acgcgatttt gttgataatc acgttttacg ctcgcggcaa ttccggcagc atccagacct
    22081 tcacactttg ggaaagtgac cgacttgctg gtttcattat tggcagcgaa aactgatgcg
    22141 caggcagaaa ccaacaacag ccctaaaaac gcccctcttt ttttcatgtt tttctccata
    22201 gcacaatgat tcaggagaaa gcatggtaca aattgtcagg agcgcaagtt gcttcaggcc
    22261 gcgtgtgagg catcttcctc ttcggattca gcaccgacga cctgtgggga cggtaatttg
    22321 ccggttttca gaatggtgct cagcacatct ttttgttctg ccagccatag cgaaagggct
    22381 tcacgttgct cgtcttccat ttgcactgga gaatttgtca gccaggtgtc aagcagatcc
    22441 gccgtatcaa gcattttatc ataagcatcg gcttcctttt tgctggtaaa cgacattttc
    22501 tcttcgccct cacgaatgac tacgtattta acttcaaccg ccatttgcag cctctcataa
    22561 taactgtgat tttatacagt atatttcttt tcggttgaga aatcaacatc agcaataaag
    22621 acacacgcaa acgttttcgt ttatactgcg cgcggaatta atcaggggat attcgttatg
    22681 acgttattag gcactgcgct gcgtccggca gcaactcgcg tgatgttatt aggctccggt
    22741 gaactgggta aagaagtggc aatcgagtgt cagcgtctcg gcgtagaggt gattgccgtc
    22801 gatcgctatg ccgacgcacc agccatgcat gtcgcgcatc gctcccatgt cattaatatg
    22861 cttgatggtg atgcattacg ccgtgtggtt gaactggaaa aaccacatta tatcgtgccg
    22921 gagatcgaag ctattgccac cgatatgctg atccaacttg aagaggaagg actgaatgtt
    22981 gtcccctgcg ctcgcgcaac gaaattaacg atgaatcgcg agggtatccg tcgcctggcg
    23041 gcagaagagc tgcagctgcc cacttccact tatcgttttg ccgatagcga aagccttttc
    23101 cgcgaggcgg ttgccgccat tggctatccc tgcattgtaa aaccggtgat gagctcttcc
    23161 ggcaaggggc agacgtttat tcgttctgca gagcaacttg ctcaggcatg ggaatacgct
    23221 cagcaaggcg gtcgcgccgg agcgggccgc gtaattgttg aaggcgtcgt taagtttgac
    23281 ttcgaaatta ccctgctaac cgtcagcgcg gtggatggcg tccatttctg tgcaccagta
    23341 ggtcatcgcc aggaagatgg cgactaccgt gaatcctggc aaccacagca aatgagcccg
    23401 cttgcccttg aacgtgcgca ggagattgcc cgtaaagtgg tgctggcact gggcggttat
    23461 ggtctgtttg gtgtcgagct gtttgtctgt ggtgatgagg tgattttcag tgaggtctcc
    23521 cctcgtccac atgataccgg gatggtgacg ttaatttctc aagatctctc agagtttgcc
    23581 ctgcatgtac gtgccttcct cggacttcct gttggcggga tccgtcagta tggtcctgca
    23641 gcttctgccg ttattctgcc acaactgacc agtcagaatg tcacgtttga taatgtgcag
    23701 aatgccgtag gcgcagattt gcagattcgt ttatttggta agccggaaat tgatggcagc
    23761 cgtcgtctgg gtgttgcgct ggcgaccgct gagagtgttg ttgacgccat tgaacgcgcg
    23821 aagcacgccg ccggacaggt aaaagtacag ggttaaaccc ggcaaaaaaa cgctacaaaa
    23881 atgcccgatc gtagatcggg cgttttgact tttacagctt agcgccttct acagcttcac
    23941 gcgccagctt agtaatgcgg tcgtaatcgc ccgcttccag cgcatctgcc ggaaccagcc
    24001 aggaaccacc gatgcacagc acgcttttca gcgccaggta gtcacggtag ttagccggag
    24061 aaataccacc cgtcgggcag aaacggacct gggagaacgg acccgcgatc gcctgcaggg
    24121 ctttcacgcc gccgttagct tcagccggga agaatttgaa ctctttcaaa ccgtagtcca
    24181 tacccagcat cagttcggaa acagtgctga tccccggaat tagaggaata gtcccttcgg
    24241 tagcagcttt cagcagcggc tcggtcagac ccgggctaat tgcgaactgt gcacccgctt
    24301 cagtgacttc tgccagctgc tgtggattca gcaccgtacc ggcacccaca atcgcttcag
    24361 gcacttcttt ggcgatagca cggatagcgt caactgcaca ctcggtacgc agagtcactt
    24421 ccagaacgcg caccccacca gcaaccaacg cttttgccat cggcactgcg tgttccagtt
    24481 tttttaccac gataaccggt acaaccgggc cggtggtcag gattgattct gcacttgttt
    24541 tccagttttt catcagagtt ttctctcgcc tgattacaaa tttgtcgtct taaaaagtga
    24601 tacaggttgc gccctgttcg gcaccggaca gtttttcacg caaggcgctg aataattcac
    24661 gtcctgtccc cacgcgtgac gcgctcaggt caggaatgcg cggttcgcga gcagccagtt
    24721 ccgcttcgtc taccagcagc gtcagttcgc ctgtctgtcc attcacacga atgatgtccc
    24781 cgtcgcgcac ttttgccagc agcccgccat cgtaggcttc tggtgttacg tggatagctg
    24841 acggcacttt acctgaagcg ccggagagtc gtccatcggt aactaacgca attttgaaac
    24901 accggtccaa taatacacca agtggcggca tgagtttatg taattctggc attccgttcg
    24961 cttttggccc ctgatgacgg acaacaacga cacaatcgcg gtccagcaaa cccgcttcaa
    25021 aggccggcat aacgtcatgc tggctttcaa aaacaaccgc tggcgcttca atcacctggt
    25081 tctcaaccgg cacggcagag gttttcataa ccgcacggcc caggttaccg cttaacactt
    25141 ttgtcccacc atgatgagag aaaggttgtt cgaaggaagc gatcacattg ctgtcgagtg
    25201 atttttccgc cccttcccgc cagtccagtt caccattatt cagccatggt tcaagggtat
    25261 aacgagacag accaaagcct gccaccgtat tgacatcttc atgcagcagg cctgctttga
    25321 gcagttcacg caccagaacc ggtacgccac ctgccgcctg gaagtggtta atatcggccg
    25381 gaccgttcgg gtagagacgt gccatcagcg gtacaacatc agaaaggtca gagaagtcat
    25441 cccagttaat ctgaataccg gccgcgcgcg ccatcgccac caggtgcatg gtgtggttag
    25501 tggaaccacc ggtcgccagc agtgcaacga taccgttcac caccactttc tcatcgatca
    25561 tcttaccgat cggcatccat tcattaccat taccggtcat gcgtgtaacc tgacgcgcag
    25621 cggcagcggt caaagcatcg cgcagcggag aatccggatg aacaaaagaa gagcctggca
    25681 actgcatccc cataaattcc accaccatct ggttggtgtt ggcagtaccg tagaaagtac
    25741 atgttcccgg cgcatggtaa gacgcggctt ctgactccag taaggccatg cggtccactt
    25801 taccttcgac ataaagctgg cgaatacgca ctttttcttt atttggcaaa ccgcttgcca
    25861 tcggtccaga cggcacaaac accgcaggca aatgaccaaa cgacagggct gccatcgtca
    25921 gacccgggac aatcttgtcg cacacaccga ggaacagagc accatcaaac atgttatggg
    25981 acagccccac cgccgcagac atcgctatca cttcgcggct tagcagcgac aattccattc
    26041 catcctgccc ctgggtgaca ccatcacaca tcgccggaac accgcccgca acctgaccaa
    26101 ccgcattcgc ttcatgcagg gctttacgaa tgatttctgg atagtgttca taaggctggt
    26161 gcgcggagag catgtcgtta taggaggtga tgatggcgat attgttacgc aacatgcttt
    26221 tcaaagaggc tttgtcttct ggctggcagg cagcgaaacc gtgtgccagg ttaccgcatg
    26281 ccaactgcga acgatgaacg gtcgacgttt tcgcttgttc tatccgggcg agataagcag
    26341 agcgagtctc gcgcgaacgt tcaatgattc gatttgttac gcgtaacaat tgtggattca
    26401 taaaggctcc tgaaattgag ttgtcagagc aggatgattc acaacgcgtt tcattcagag
    26461 gatttatgag tgaaacgcct gtaaccggag ctcatagggc aaaaacgttt cagtcagtgt
    26521 aataaaaaaa gcctcgtggg tgaatccgca cgaggcctga aagtgtaaaa attgttctac
    26581 aatctgtgca agatcatgtt accggtaaaa taaccataaa ggataagcgc agatattact
    26641 caaactcatt ccaggaacga ccatcacggg taatcatcgc caccgaggca acgggtcccc
    26701 aggttccggc ctgatacggt ttcggcgcat cattgtccat cgcccacgcc tcagtaatgg
    26761 agtctaccca tttccaggct tcttccactt cgtcgcgacg aacaaacagt gcctgaatac
    26821 cacgcatggt ttccagcagc aaacgttcat aggcatccgc cagatgcgtc tgattaaagg
    26881 tttctgaata gctcagatcc agcttggtga tttgcaggtt atgtttgtgg tcaaggccag
    26941 gaactttatt cagtacctgg atatccacgc cttcatcagg ttgcagacgg atagtcagtt
    27001 tattctgcgg cagatcctgc cacgactctt taaacagatt cagttcaggt gttttgaaat
    27061 agaccacgac ttcagaacat ttggtcggca gacgtttacc agtacgcagg tagaatggca
    27121 caccggccca gcgccagtta tcaatgtcga cgcggatcgc cacgaaggtt tctgtattgc
    27181 tgctcttgtt cgcgccctct tcttccagat atcccggcac ttttttgccc tgggcgaagc
    27241 ccgcagtata ttgcccgcgt acggtttttt cgcgtacgtt ggagcggtcg atgcggcgca
    27301 gagacttcag tactttcact ttttcatcgc ggatgctgtc tgcgctcagg tcagacggcg
    27361 gagacatcgc aatcatgcaa agaatttgca gcaggtggtt ctggatcatg tcgcgcatct
    27421 gaccggcttt atcaaaatag ccccagcgcc cttcgatccc cacttcttct gccacggtaa
    27481 tctcaacatg atcaatggtg cgattgtccc agttattcac aaacagggag ttagcaaaac
    27541 gcagcgccaa caggttcagc accgtttctt taccaagata gtggtcgata cggtaaacct
    27601 ggcactcctc gaagtattcg ccaacctgat cattgatttc ctgcgaggtc gccagcgacg
    27661 tccccagcgg tttctccatg actacgcgtg ccggtttagc attcagtttt gcctcgccaa
    27721 gccctttgca aattgcgcca aaagtgctgg gcggcatggc aaagtagtta atggtgatac
    27781 gatttttttg atccagcatc gcgccgagac ggctgaatgc agcagtgtca ttgacatcga
    27841 gattacaaaa atccagacgt gcactcaggg tgtcccataa accttcatca atggtttctt
    27901 tcatgaaagt ttcgagcgcc tcgcggacaa ctttggtata tgccgcttta tcccagtcag
    27961 cacgccctac gccgataatc cgggtgtccg ggttgagctg accggctttt tccagttgat
    28021 acagggaagg cagcaattta cgacgcgcaa ggtcgccttt cgcgccgaaa atgaccaggt
    28081 cacaggcctg ggctgtttgc gttaccgcca tgtcattctc cttaagttaa ctaacccggt
    28141 acttaagcca gggtatactt gtaattttct tacggtgcac tgtactgctt ttacgagctt
    28201 gcgaaaactg taaacgctta tccacccgtg cgattacggg aaaagcgcgc aaagtgcggc
    28261 aaaactgata aaaaaatcat cgttttccat tgggtaaaaa tctgacactg atcatgttat
    28321 gaaaaaaaat aacaactttt ttatctgctt ttgtcattaa cacggcacac aggcgtaata
    28381 tctgacaaaa ctgcatttcg atttctttca gtgcggaaat cgtcattacc cgtgagtctc
    28441 tttacatcat gaatatgctg gaaaaaatcc agtctcagct ggaacatttg agcaaatcag
    28501 agcgcaaagt tgccgaggtc attctggctt cgcccgataa cgcgatccat tcgagtattg
    28561 ctgctatggc actggaagcc aatgttagcg aaccgacggt gaatcgtttc tgtcgcagca
    28621 tggacacgcg cggttttcct gattttaaac ttcatctggc acagagtctg gcgaatagca
    28681 ctccctatgt taatcgcaat gtcaatgaag atgacagcgt tgaatcatac acagggaaaa
    28741 tatttgagtc cgcaatggca acgcttgatc atgtccgtca ttcactggat aaatctgcca
    28801 tcaaccgcgc cgtcgacttg ctcactcagg caaaaaaaat cgcctttttc ggattaggct
    28861 cttcagccgc cgttgcccac gatgcgatga ataagttctt tcgttttaat gttccggtgg
    28921 tgtactccga tgatatcgtg ctgcaacgca tgagttgtat gaattgtagc gacggagacg
    28981 tggtggtgct gatttctcac actggaagaa caaaaaatct ggtcgagctg gcgcagctgg
    29041 ctcgcgaaaa cgacgccatg gtgattgccc tcacctctgc gggtaccccg ctcgcccggg
    29101 aagcaacgct ggcaattacc ctcgacgtac cggaagatac tgacatttat atgcccatgg
    29161 tttctcgact tgcacagctg accgtgatag atgtgctggc gacaggattt actttgcgac
    29221 gcggtgcaaa attcagagat aacttgaagc gggtcaagga agcgctgaag gaatcgcgtt
    29281 ttgataagca gttacttaat ttaagtgacg atcgctaaaa acgactgtca ctgtcctaat
    29341 cttatacgac atccgaatga gattaattta tcgccatcgc ggcgttattt cattcggatt
    29401 tcatgttcaa gcaacacctg gttgtttcag tcaacggagt attacatgtc cagaaggctt
    29461 cgcagaacaa aaatcgttac cacgttaggc ccagcaacag atcgcgataa taaccttgaa
    29521 aaagttatcg cggcaggtgc caacgttgta cgtatgaact tttctcacgg ctcgcctgaa
    29581 gatcacaaaa tgcgcgcgga taaagttcgt gagattgccg caaaactggg gcgtcatgtg
    29641 gctattctgg gtgacctcca ggggcccaaa atccgtgtat ccacctttaa agaaggcaaa
    29701 gttttcctca atattgggga taaattcctg ctcgacgcca acctgggtaa aggtgaaggc
    29761 gacaaagaaa aagtcggtat cgactacaaa ggcctgcctg ctgacgtcgt gcctggtgac
    29821 atcctgctgc tggacgatgg tcgcgtccag ttaaaagtac tgaaagttca gggcatgaaa
    29881 gtgttcaccg aagtcaccgt cggtggtccc ctctccaaca ataaaggtat caacaaactt
    29941 ggcggcggtt tgtcggctga agcgctgacc gaaaaagaca aagcagacat taagactgcg
    30001 gcgttgattg gcgtagatta cctggctgtc tccttcccac gctgtggcga agatctgaac
    30061 tatgcccgtc ggctggcacg cgatgcagga tgtgatgcga aaattgttgc caaggttgaa
    30121 cgtgcggaag ccgtttgcag ccaggatgca atggatgaca tcatcctcgc ctctgacgtg
    30181 gtaatggttg cacgtggcga cctcggtgtg gaaattggcg acccggaact ggtcggcatt
    30241 cagaaagcgt tgatccgtcg tgcgcgtcag ctaaaccgag cggtaatcac ggcgacccag
    30301 atgatggagt caatgattac taacccgatg ccgacgcgtg cagaagtcat ggacgtagca
    30361 aacgccgttc tggatggtac tgacgctgtg atgctgtctg cagaaactgc cgcagggcag
    30421 tatccgtcag aaactgttgc agccatggcg cgcgtttgcc tgggtgcgga aaaaatcccg
    30481 agcatcaacg tttctaaaca ccgtctggac gttcagttcg acaatgtgga agaagctatt
    30541 gccatgtcag caatgtacgc agctaaccac ctgaaaggcg ttacggcgat tattaccatg
    30601 accgaatcgg gtcgtaccgc gctgatgacc tcccgtatca gctctggtct accaattttc
    30661 gccatgtcgc gccatgaacg tacgctgaac ctgactgctc tctatcgtgg cgttacgccg
    30721 gtgcactttg atagcgctaa tgacggcgta gcagctgcca gcgaagcggt taatctgctg
    30781 cgcgataaag gttacttgat gtctggtgac ctggtgattg tcacccaggg cgacgtgatg
    30841 agtaccgtgg gttctactaa taccacgcgt attttaacgg tagagtaagt acgttgccgg
    30901 atgcggcgaa aacgccacat ccggcctaca gttcaatgat agttcaacag atttcgaata
    30961 ttctgaagca aacttgaact tatcatcagg cgaaggcctc tcctcgcgag aggctttttt
    31021 atttgatggg ataaagatct ttgcgcttat acggctggat ttcgcccggt ttgcgagttt
    31081 tcagcaattt tagtatccag gtgtattgtt ctggtcgcgg accaacaaaa atctcgactt
    31141 cttcattcat ccgccgcgca atcgtatgat catccgcctc taacagatca tccatcggtg
    31201 ggcgcacctg aatcgtcaga cggtgcgtct tgccatcata aatcggaaac agcggaacaa
    31261 cgcgcgcacg gcacactttc atcaaacgac caatcgcggg caacgtcgct ttataggtgg
    31321 caaagaaatc aacaaattcg ctgtgctcag ggccatgatc ctgatcgggt aaataatatc
    31381 cccagtagcc ctgacgtacc gactggatga atggtttaat accatcattt ctcgcatgca
    31441 gacgaccgcc aaaacgacga cgcaccgtgt tccagacata atcaaaaacc gggttgccct
    31501 gattatggaa catcgctgcc attttctgcc cttgcgaggc catcagcatg gcaggaatat
    31561 cgacggccca accgtgcggc accagaaaga taactttctc gttattacgc cgcatctctt
    31621 cgatgatctc cagcccttgc cagtcaacgc gcggctgaat tttctccggc ccgcgtattg
    31681 ccaactcagc catcattgcc atcgcttgcg gcgcggtggc aaacatctca tcaacaatcg
    31741 cttcgcgttc agcttcacta cgttctggaa agcagagcga cagattgatt aacgcacgac
    31801 ggcgtgagct ttttcccagt cgtccggcaa aacgtcccag ccgtgccaga atgggatcac
    31861 ggaactttgg cggcgttaaa gcgatacccg ccatcgctgc tacgcccagc catgctcccc
    31921 agtagcgcgg gtggcgaaag gatttatcaa actcaggaat gtattcgcta ttattttttt
    31981 tcgtttccat gcttttccag tttcggataa ggcaaaaatc aatctggtga tagtgtagcg
    32041 gcgcaacttg ccccgcacca aataaaaaaa gccggtactg acttcgtacc ggctgcgaat
    32101 ggatgttaat taatcaaacc gtagctgcgg cacaatctct ttggcctgcg ccaggaattc
    32161 gcgacgatcg gagccggtca gcccttcggt acgcggcagt tttgccgtca gcgggtttac
    32221 ggcctgctgg tttatccata cttcatagtg caaatgcggc ccggttgaac gtccggtatt
    32281 accggaaagc gcgatacggt cgccacgttt caccttctgt cccggtttca ccagaatctt
    32341 gcgcaagtgc atataacgcg tggtgtagct gcgaccatga cgaatagcca cataataacc
    32401 tgctgcgcca ctgcgtttgg caaccaccac ttcaccgtca cccactgaaa gcactggcgt
    32461 accctgcggc atggcgaaat caacacctct gtgtggtgca acgcgaccgg tcaccggatt
    32521 agtacgacgc gggttaaagt tagacgagat acggaactgt ttcgccgtcg ggaatcgcaa
    32581 gaatcctttc gccagaccag taccgttacg atcgtagaat ttgccatctt cagcgcggat
    32641 agcgtaataa tctttacctt ctgaacgcaa acgtacgccc agcagctggc tttgctcacg
    32701 tttaccatca agcatttctc gagacattaa taccgcaaat tcatcgcctt ttttcagttt
    32761 gcggaaatcc atttgccact gcatggcttt aatcactgcg ctcacttcgg cgctggttaa
    32821 accggcgttt ctggcgctgg caacaaagct ccccccgacg gtacctttca gcagattgtt
    32881 aacccactct ccttgctgca tttcgctggt cattttaaaa ccgttagcgg cagtacggtc
    32941 ataggttcgg gtttcacgac gagacacttc ccaggtgagg cgctgcagtt cgccgtccgc
    33001 ggttaatgtc caggagagtt gttgaccgat tttcaggtta cgcaattctt tgtcggcagc
    33061 agccagttgg gtgatatcac ccatatcaat accatactga ttgagaatgc tgcttagcgt
    33121 atcgccagtg gaaacaacat attcatgcac gcccgcttca ccggcgattt tgtcatccag
    33181 ttcgtcctga ggaatggctt catcttcttg tgcagcttga tcaatcggct cactggcttc
    33241 aggtaagagc gaacgaattt cgttctgttc cagctcaatg gttttgacaa ttggcgtggc
    33301 atcgcggtga taaacatagg gccgccagac agcgacggcc agagtaagaa cggtgagcga
    33361 ccccaacata acgcggtgtg gtcgcggtaa attattaaac gccagggcga cagagcgggc
    33421 tatctgttgc acgtaatcac ttcctcatta atctcctttc aggcagctcg catactggtt
    33481 ggctaattga ctcaggaatt ctgaatagct tgttttaccc agtttgatat tcgtccccag
    33541 gggatccaac gttcccatac gaacggatgt ccctcgtgcg acgctctcaa cgaccgctgg
    33601 cctgaactgt ggctcagcaa aaacgcaggt tgctttttgc tcaaccaact gtgttcttat
    33661 ttcatgtaaa cgctgcgcgc caggttgaat ctcagggtta acggtaaaat gaccaagcgg
    33721 tgtcagtcca aactgttttt cgaaatagcc gtaagcatcg tgaaaaacga aataaccttt
    33781 ccccttgagc ggcgcgagct cgttaccaac ctgcgtttcg gttgaggcta attgtgcctc
    33841 aaaatccttc aggttggcgt caagtttggc tcgactttgc ggcataagtt ccactaattt
    33901 tccatggatt gcaaccgctg tagcccgcgc tatctctggg gaaagccaaa gatgcatgtt
    33961 gaaatcgccg tgatggtgat cttcgtcact tttttccgcg tggtcgtgat catcatcatc
    34021 gccgtgaata cttttcatca gtagcggttt cacatcttca agctgcgcaa tcgttacctg
    34081 cttcgcttct ggtaatttgc ttaccggttt ttgcataaac gcttccatct ccgggccaac
    34141 ccaaacgact aagtccgcgt tctgtaagcg ttttacatcc gatggacgca gtgaataatc
    34201 atgttctgaa gcgccgtcag gcagtaaaac ctccgtttct gttacaccat cagcaatggc
    34261 agaagcgatg aacccaacgg gtttaagcga agcgacaacg gcggcatctg cggcctgtgt
    34321 tgcacctccc cagagagcgg cggataatgc tgcgaaaaga agcgtttttt tatgtaacat
    34381 aatgcgacca atcatcgtaa tgaatatgag aagtgtgata ttataacatt tcatgactac
    34441 tgcaagacta aaattaacat gacaagtctg gtttccctgg aaaatgtctc ggtttctttt
    34501 ggccaacgcc gcgtcctctc tgatgtgtcg ctggaactta aacctggaaa aattttgact
    34561 ttacttgggc caaatggcgc aggtaagtcg acactggtac gggtagtgct cgggctggta
    34621 acacccgatg aaggggttat caagcgcaac ggaaaactgc gcatcggcta tgtaccgcag
    34681 aagctgtatc tcgacaccac gttgccactg accgtaaacc gttttttacg cttacgccct
    34741 ggtacacata aagaagatat tttgcctgca ctgaaacgtg tccaggccgg gcacctgatt
    34801 aacgcaccga tgcaaaagct ctctggtggc gaaacgcagc gtgtactatt agcgcgagca
    34861 ttgttaaatc gcccgcaatt attagtgctg gatgaaccca ctcaaggcgt ggatgtaaat
    34921 ggccaggtgg cgttatatga ccttattgac caactgcgtc gtgaactgga ttgtggcgtt
    34981 ttaatggttt ctcacgatct gcatctggtg atggcaaaaa ccgatgaagt gctgtgcctg
    35041 aatcaccaca tttgttgttc cggcacaccg gaagttgttt ccctgcatcc ggagtttatt
    35101 tcaatgtttg gtcctcgtgg tgctgaacaa ctgggtatct atcgccatca tcataaccat
    35161 cgtcacgatt tacagggacg aattgttttg cgtcggggaa atgatcgctc atgattgaat
    35221 tattatttcc cggttggtta gccgggatca tgctcgcctg tgccgcgggt ccgctgggtt
    35281 cgtttgtagt ctggcgtcgt atgtcttatt tcggtgatac gctggctcat gcctcattac
    35341 ttggcgtcgc gtttggtttg ttgctggacg tgaatccatt ctatgcggta attgccgtta
    35401 cgctgctcct ggcgggcggt ctggtatggc tggagaagcg tccacagctg gcgattgaca
    35461 cgttattagg gattatggcg cacagtgccc tgtcgctggg cctggtggtc gttagtctga
    35521 tgtctaacat tcgtgttgat ttgatggctt acctgttcgg tgatttgctg gcagtgacgc
    35581 cagaagatct catcgctatt gcgattggcg tggtcatcgt ggtggctatt ttgttctggc
    35641 aatggcgcaa tttgctgtcg atgacgatta gcccggatct ggcgtttgtt gatggtgtga
    35701 aattacagcg cgtgaaattg ttgttgatgc tggtgacggc attgacgatt ggtgtagcga
    35761 tgaaatttgt tggcgcgttg attattactt cactgctgat tattcctgca gctactgcgc
    35821 gtcgctttgc ccgtacgccg gaacagatgg ctggtgtcgc tgttttggtg gggatggtgg
    35881 cagtaactgg cggtttaacc ttttccgcgt tttacgatac gccggcgggt ccgtcggtgg
    35941 tgctatgtgc ggcactgtta tttattctca gtatgatgaa aaagcaggcc agctaatcta
    36001 tcgctgaaca catttgtcgg atgcggcgcg agcgccttat ccgacctacg gttcgctatc
    36061 tctggtaggc cggataagac gcgaatagcg tcgcatcagg cacactgcca atgtcggatg
    36121 cggtgcgagc gaccaatccg acttacggca tttctggcgg cgttatgcca aagtgattcc
    36181 acgcccgcgt cgtcgccata cgcccacgcg gtgtacgctg caaaaagcct tgctgaatca
    36241 gataaggctc cagcacatcc tcaatggttt cacgttcttc gccaatagcc gccgccaggt
    36301 tatccagacc tacaggccca ccaaagaatt tatcgattac cgccagcaac aatttgcggt
    36361 ccatataatc aaaaccttca gcatcgacat tcagcatatc cagcgcctga gcagcgatat
    36421 ctgccgagat ggtgccatcg tgcttcactt cggcgaaatc acgcactcga cgcagcagac
    36481 ggttggcaat gcgcggcgta ccgcgagcgc gacgagcaac ttccagcgcg ccgtcatcac
    36541 tcatctcaag ccccataaag cgtgcgctgc gactgacgat atattgcaga tccggcacct
    36601 gataaaactc cagacgttgc acaataccaa aacggtcgcg caacggtgat gtcagcgaac
    36661 ctgcgcgtgt ggttgcacca atcagggtaa acggcggcaa atcaatttta atggagcgtg
    36721 ccgccggacc ttcaccaatc atgatatcca gttggtagtc ttccattgcc ggatacaaca
    36781 cctcttcaac aactggcgat agacggtgga tctcatcaat aaacagcacg tcatgcggtt
    36841 caaggttagt gagcatcgct gccagatcgc ccgccttttc cagcaccgga ccagaagtcg
    36901 tgcgtaaatt aacgcccatt tcattggcga caatgttggc aagcgtagtt ttacccaacc
    36961 ccggaggacc aaaaatcaac aaatgatcga gggcatcgcc gcgcagtttc gctgctttga
    37021 tgaaaatctc catctgtgaa cgaacctgcg gctgaccaac atactcttcc agtaatttag
    37081 ggcgaatggc gcgatctgct acatcttccg gcaaagtggt accggcagaa atcagacggt
    37141 ctgcttcaat catcctttac ctcataacgc ggcgcgtagg gcttcgcgaa ttaatgtttc
    37201 actgctggcg tcagggcgag cgattttgct caccatgcgg cttgcttctt gtggtttata
    37261 gcccagcgcc accagcgcgg caaccgcttc ttgttcagca tcgtcggtcg ccgggctggc
    37321 aggagacgtg agcaccagat cggcggctgg cgtaaagaga tcgccgtgca aacctttaaa
    37381 tcggtctttc atttcgacaa ttaaacgctc ggcggttttt ttaccaatgc ccggcagttt
    37441 caccagtgct cccacttctt cacgttcaac ggcattaacg aactgctgcg ctgacattcc
    37501 ggagaggatc gccagcgcca acttcgggcc gacgccgttg gttttgatta actctttaaa
    37561 caacgtgcgc tcttgcttat tgttaaagcc gtacagcagc tgcgcgtctt cacgcaccac
    37621 aaagtgggtg aaaacgatcg cttcctgacc cgcttcaggg agttcataaa aacaggtcat
    37681 cggcatatgc acttcatagc ctacgccgcc cacttcaatt aacaccagcg ggggttgttt
    37741 ttcaatgatg atgcctctga gtctgcctat cacatgacgc tcctgcgtaa tgaatcaaag
    37801 ataatgctgt atgataaaaa aatgctggat agatatccag cgaaggatga agaaaacttg
    37861 cgaggcgtcc caacgatctg aaaaatggcg cagtataatt tattctacag gttatattgg
    37921 aagcaa
//
LOCUS       DAZGVQ010000029        37649 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000029 DAZGVQ010000000
VERSION     DAZGVQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.029"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(562..702)
                     /locus_tag="ACHLAG_001440"
     CDS             complement(562..702)
                     /locus_tag="ACHLAG_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0654700.1"
                     /translation="MPQKTIIVSILCLTMLLTVWMIHRSPCEFRLNIMWSEFAAFLQC
                     KP"
     gene            complement(1004..1282)
                     /gene="rpmE"
                     /locus_tag="ACHLAG_001441"
     CDS             complement(1004..1282)
                     /gene="rpmE"
                     /locus_tag="ACHLAG_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFP0654701.1"
                     /translation="MISISNEITSSSTCVDEYFRIGSTIKTDRVIELDGVTYPYVTID
                     VSSKSHPFYTGKLRTVASEGNVARFTQRFGRFGRFGRFGRFVDAKKGG"
     gene            1433..1717
                     /locus_tag="ACHLAG_001442"
     CDS             1433..1717
                     /locus_tag="ACHLAG_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654702.1"
                     /translation="MKNEAEKAMWSCKTSDTPWTFSSLLKKAVCAILPDAEPKIENAT
                     KGCSVLSTVDNAPGNKSLNYDPFSFESGLHLDIENLPLIENWDGEPYKLP"
     gene            complement(1763..3265)
                     /locus_tag="ACHLAG_001443"
     CDS             complement(1763..3265)
                     /locus_tag="ACHLAG_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFP0654703.1"
                     /translation="MTTNNYLEYFLTLLGWLVNNGLWDLLIGTGLFALPLAFKVIGIW
                     LKVREEGADEGNKGMLSLPRIENALYGAFFVMVASCVPLVQVTLDTLKFDRSRAQTCG
                     VWTPKAPGDTGYQGIISSLGDKTAAAPIWWVVVHKLSKGVTQAAVASIPCRPDLRQLR
                     FEVQRTFIANRALADELQDFTNDCYSLAMYQWKQRDQGMTKDRKVLSDISWIGSSTFM
                     QGDYGTLQSRMPRAAFPWNDARDSGRPDTGRGGYPTCREWWNDSSVGLKKRVTEEVDE
                     GLWLRLSAAMKLLGNSQATYQEEIIKRLVSPANLTISQGGEVYRGYGGNADMTTMNAI
                     SRIGATAGSGVASLGAFPAFDAMRQALPMVQSILLMAVYIMLPMILAFAAYEFSTLIT
                     LTFVVFALNFLTFWWELARWLDSWMITALYGSDTHSSLNMMGLQNTSDDIIMNLVMGT
                     MFIVLPAVWLGALSWAGVSIGQVINAAIGKGVSTSQQAGQHAGGITNKLK"
     gene            complement(3269..3634)
                     /locus_tag="ACHLAG_001444"
     CDS             complement(3269..3634)
                     /locus_tag="ACHLAG_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654704.1"
                     /translation="MNKTVLMVPGSRAGLLVKGMKLLGKWGLTVIVIMASALLIADTG
                     MRHAQEVDALRLWFHETRFGWLGWRFCIYCGLVWGCRKIWRAPGFRAEYRLRFARICM
                     VSALFFLLCEYAIFSAAGD"
     gene            complement(3627..5048)
                     /locus_tag="ACHLAG_001445"
     CDS             complement(3627..5048)
                     /locus_tag="ACHLAG_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFP0654705.1"
                     /translation="MTMKVKTLVMMVMLSCSTPVMADSILSLPKLNDSTFGYGKSVDG
                     AVSDKLFYTLGGGSVISQPATRSNMQKLGLDLGWSSDLMCGNFDLKTTVGNQLNGITD
                     GFKNLMGDVIQGATGAVSSLPAMVIQRANPGLYDMITNGVLQANVAFDKAQLNCQNMA
                     KRMMDFSDRSKWTQAAMMEEYKKVVNTSDGDAVKGDDAGRQATGKEGQKWIGGQQRGG
                     AGQPAIRPVHDMTAAGFNMMNSLPVTSTSGVGAGSCNGSACEKFRNAEEAASAVVKVL
                     GDRSMRTCTDAKECTSGDSDQQPGTAVAGTGFAPMLEEATRINTEQLVRLVNGQDKPT
                     AENLAKLKTGSLAVSAGVIHALRRDPDNMSLTSRLAGELAMADTVETALVMRRMLLTG
                     MSEPYAAAQPAALEEGDRRIASLDREIIALKSEMELKRDLARNSVLTIIERDNERVSN
                     NPMIQQTDNADSRVRSLEVPENE"
     gene            complement(5060..6034)
                     /locus_tag="ACHLAG_001446"
     CDS             complement(5060..6034)
                     /locus_tag="ACHLAG_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFP0654706.1"
                     /translation="MMSRSFRLRAGALLLSVAALPLASASLNTASIVASAAAPDCISW
                     RVSGICYWLYCSGFSCKVRTSVKVTHYIPEVVVSTYTAPGGNPWKEMSLVSQTAGGLE
                     NAITKGLAGLSAGGGNPADMKAQGERRSAIRFKYADALGHPATRLIGGMVPGFSCDSP
                     VTPAMPYFLSTLDSLVWRTGMPESLYPEALVPGKREIGSQAEENLWGSVYPRSGFVMQ
                     TDDDKAAAVVAQRVADIISRTGEPHVYKSIQTPKKDGFWPPDPVEENTGTRNHKWQRL
                     TPSVSRSCAVFPDGDHQAAEDGNAAFALWQPYSCCKKRGQRFLGSTNF"
     gene            complement(6031..6429)
                     /locus_tag="ACHLAG_001447"
     CDS             complement(6031..6429)
                     /locus_tag="ACHLAG_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HFP0654707.1"
                     /translation="MHRIIFCSLFFLPMAVSAGITLYTDSQHLPVNPPADVRVILLDG
                     PEQLQARFFGALPADAAEAEARVRARMQSPEWGAMQAELAERYREVAYAWSLGVKKYP
                     AVVFDDREVVYGTTDVAEAAQLRAQEGVQP"
     gene            complement(6694..7080)
                     /locus_tag="ACHLAG_001448"
     CDS             complement(6694..7080)
                     /locus_tag="ACHLAG_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP0654708.1"
                     /translation="MMYLKYPEPELQTAVHPGGVVFFLPPQGKPGVTEITQPHLLRLR
                     SHLNRRLGRLRRVVCQPQRVGMNSSVAVTLEGDTGKVYLLVSVCGHECWPSEEEYTHL
                     RWYIQVVDAADLFYLLLWLNGMEAEK"
     gene            complement(7077..9773)
                     /locus_tag="ACHLAG_001449"
     CDS             complement(7077..9773)
                     /locus_tag="ACHLAG_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFP0654709.1"
                     /translation="MTQEEEVRVYHAGPSVIDFLPWVEYLDEAQCLLLDDGMSVGAVY
                     DVTPAATEGRTEERLEQIRDMVEDALQDSFDEYDTHPWVVQFFCQDENDVDAYLDYLK
                     GYVKPHAQGTAFTEAWLGEMERHLRGIARPEGLFTDTLVTGQPWRGQQRRTRMVIYRR
                     IAKNNSDPMPPVAMLNQVCDRVVSALGSAGVRCHRMNGLQVHSWLLRLFNPAPSWISR
                     EDLYRMASRADPRENAADVMPVMTDFAESLWFTPPHSDPENGVWWIDDLPHCAVVVEK
                     LRTPPEPGAITGEMSRGEKTVNALMDTFPEGTVLCMTIVVQPQDTLEERFTRLSKNAV
                     GENTESIRARQDVKVVKELLGNRHKLYRAGITFLLRAGDMDSLKRKRVELTTVLLGAG
                     LQPVRPEFDVGPLNSWLRALPMCFDPDMDRKQWHTRLMWVQHLAGLLPVTGRETGTGH
                     PGFSFFNRGGDVLTFDPLNKLDRTQNAHLLLFGPTGAGKSATLCASLSQVMAVHRPRL
                     FIAEAGNSFGLLADYFESLGLSVNKISVKPGSGVTLPPFADAHKLVEEGQTLQSVDEH
                     SLPDLDGDEEEEKRDILGEMEISARMMITGGDPKEEASLKRADRAMIREALLMATHAT
                     YREGRQMLPEDLQVALREISCDAGRNEVRRAKAAEMAESLGMFTQKGSFEAELFNREG
                     ELWPEADVTLIDLGHLAREGYEAQMALTMVSMTNMINNIAERDQYLGRDIVFAVDEAH
                     IVTVNPLLSPYMTKVVKMWRKLGAWLWLATQNLKDYPDISEKMLNMAEWWVCLTMPPE
                     EVNNIARFKSLTEEQKAVLMSASKLSGCYTEGVVLAKKIEALFRAVPPSLYLALGMTE
                     KDEKAERRMLMKEHNCSELEAAFHVARKLDRLRGLSAVESAA"
     gene            complement(9893..10285)
                     /locus_tag="ACHLAG_001450"
     CDS             complement(9893..10285)
                     /locus_tag="ACHLAG_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFP0654710.1"
                     /translation="MCIPLTALLLSGCSTSKEELLPAGDQTMLELWQGKTTGGSQRHA
                     LAARETLWRPLTSDEKQQVHREGQSYSRTQESEISQQFPRLPNPDMVMYVFPHLAGGT
                     SPVPGYSTVFPFYSRTQYALPGERTEAM"
     gene            complement(10324..11802)
                     /locus_tag="ACHLAG_001451"
     CDS             complement(10324..11802)
                     /locus_tag="ACHLAG_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFP0654711.1"
                     /translation="MQIKSNALVKFVVPAVVLVGLAVTMKSCKTQENVADRGEQARAL
                     SGLSSEELRALGVEGDTPEDTLRTLVARMNQVRDNQRTLEKQNSQLLKDNEQLRRRST
                     DVSGQVNDAVAGVQKTYEQKQRELQEQQHSLMSQIDAMKDQLSRAETLSRRQESDIPP
                     GLGLDAGGGSGVVISQGADGLMWVSPADQSQDDPLSSSGSKGGPRFPTSFLEDNALSR
                     QKAEYEQQVKGYSNEQQRATTQAVYTLPENSTLTGSRAMTALLGRVPINGTVTDPYPF
                     KILVGRDNLTANGIELPDVEGAIVSGTASGDWTLSCVRGQVNSITFVFADGTVRTLPS
                     PDVNGTTNSNQQAKGNGTGGIGWISDEDGIPCISGTRKSNASSYLPTIFALSAAGAAG
                     DALSEGQYTSQNNVNGITSTLTGSAGQAVLGKALSGGMNETADWVRQRYGQTFDAIYV
                     PPGKALAVHITRQLAIDYEEQGRRVRYDFALPGEANDHGGLD"
     gene            complement(11792..12700)
                     /locus_tag="ACHLAG_001452"
     CDS             complement(11792..12700)
                     /locus_tag="ACHLAG_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFP0654712.1"
                     /translation="MKAGIVCSLLRPAVMMMSLSVLSLTAQADELMKWERIPLNIPLK
                     VGQERVIFADKNVRVGFPPALNGKLRVQSTGGAVYLKADSAFPQTRVQLQDVESGEMI
                     LLDVTAGEKGTTEPVRIVYDGDVSTVSRAAGSDVQTVKSRSSNGKPGTDNSTQARRTE
                     PRYAEPLPVLLTRYAAQSLYAPLRTLEPVAGIRPVVPHLPRQITTVYPSEPLTVSPVA
                     AWAAGGRTVVALKLTSTVSRRIVLDPRALQGRFVAATFQHRWLGAAGTPEDTTMLYVV
                     TAGSPEKAFVEEPVTSRRGGEVTHAD"
     gene            complement(12697..13344)
                     /locus_tag="ACHLAG_001453"
     CDS             complement(12697..13344)
                     /locus_tag="ACHLAG_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFP0654713.1"
                     /translation="MSRFRHALKDRDQHILTLRVFSGVLCGLLLMVTIGWMTAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAFYIFQQLNAWPKNGEVDYPAKIHALSAYLTP
                     SCREILETDARRRKDAGELLDRVRVVYEIPEHGYGGRYNRDPVTVEGRDNWLVRLDLV
                     ADEYFHAEPVKRALIRYPMKVMRMEGDPERNPFGLVLDCYSSTPQRLEAVKGGGE"
     gene            complement(13341..13706)
                     /locus_tag="ACHLAG_001454"
     CDS             complement(13341..13706)
                     /locus_tag="ACHLAG_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFP0654714.1"
                     /translation="METIRFLPDRLNAEPTVFRGFTTPELGLAALTGFFTGLLWSLPF
                     VPVFGWVIIPTGALLTPLVLVWTGGAWISRLKRGKPDNWLWQRLEEKKAALGVGRSGL
                     IREARGWSLRRTRVRGGKS"
     gene            complement(13726..14064)
                     /locus_tag="ACHLAG_001455"
     CDS             complement(13726..14064)
                     /locus_tag="ACHLAG_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFP0654715.1"
                     /translation="MARLAGMAMVTFLLGSLTPALADLPQIEPPSSGGGGGLFGQIKG
                     YFQDGIVLIGLVIAAAAFVKVAEAAVTTFSEVRDGKTGWGQFGAIIVVGVVLLVAVIW
                     LLGKSASIIL"
     gene            complement(14134..14376)
                     /locus_tag="ACHLAG_001456"
     CDS             complement(14134..14376)
                     /locus_tag="ACHLAG_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFP0654716.1"
                     /translation="MALTSAQAAAFKVASGDVDLNVLHLSLLGLLIAVLFLWAAWGLS
                     DVWSGWRNGDVRESAFIWFTARTVLLLVASIWMFAG"
     gene            complement(14376..14660)
                     /locus_tag="ACHLAG_001457"
     CDS             complement(14376..14660)
                     /locus_tag="ACHLAG_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016190711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFP0654717.1"
                     /translation="MMLLVTLVLPVQAAEKDELATVMRQLDQVRAALDRARTASLQAT
                     PHDGRYFFDYPRAQADIQTMQEGIDRYLTPSRAQPRNGSAVTGEYRRERP"
     gene            complement(15350..16111)
                     /locus_tag="ACHLAG_001458"
     CDS             complement(15350..16111)
                     /locus_tag="ACHLAG_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFP0654718.1"
                     /translation="MAEARRDAERSPQSRPPRQHGLLYNLVWGWPWALVGLLLSSLLL
                     SLLIEYAGMTFFWPDEGASHSRAVMNTELGWLSTEFTRSLLLSTPSVTVPGWISVAYQ
                     WLFVESGMQSWLQSQQAVTAAGRSVSQFSGELSQWLAYHLHDYLLATVYVTVVTMVRV
                     IILALSVPLFVMVILVAVVEGLGRRDLRRYGAAYESSFVYHHAKKSIKPAIYIPCILY
                     LSWPTAVYPNLLIWPAAMLLGMAVTVTMASFKKYL"
     gene            complement(16092..18209)
                     /gene="traD"
                     /locus_tag="ACHLAG_001459"
     CDS             complement(16092..18209)
                     /gene="traD"
                     /locus_tag="ACHLAG_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFP0654719.1"
                     /translation="MSNRYVIEALLRPAVEFNTAVVAATAAGICVTAPWAVALAPSVS
                     YVTAAGFGVLAAVRFRQGMKIIRYRRNLRRLPRYVMSTRQIPVSRQRLFLGRGFRWTQ
                     KHTQRLQDTLRPEVAHYLQPGGLYRAARWLEMKTEHRLPGLGQWLRRDSPLNPVRPLP
                     PVGGNPALHGIEPDEQDVTLALGERVGHTIVYGTTRVGKTRLAELLVTQDIRRGEVTI
                     VFDPKGDADLLLRVWAEAHRAGRGDELYIFHLGWPEISARYNAVGRFGRVSEVASRVA
                     GQLSGEGNSAAFREFAWRFVNIIARALVALGERPDYMLIMRYVNNIADLYIRYAGKVI
                     RERLPGLEQIIANNQSVLSEDDVPRTMQNQPDAVRIWSIEMALSSEEGKKIYDPILDG
                     LRSAVRYDRTYFDKIVASLLPLLEKLTTGRIAELLAPDYLDMSDARPIFDWEQVIRKK
                     AVVYIGLDALSDSEVASAVGNSMFADLVSVAGHIYKHGMHAGLPAGGAGKNVVNLHCD
                     EFNELMGDEFIPLINKGGGAGMQVTAYTQTSSDIEARIGNAAKTAQVQGNFNNMIMLR
                     VRENRTAELLTTQLPQVEIYTKTLVSGHQDTADVSAGQDFTSSTQDRVGTVKVPLLEP
                     ADIVTLPKGQAFALLEGGQLWKIRMPLPAGDTDDVLMPESISRVAEAMRQNYHSGEGW
                     WNAPPSVQAIATEEGGDHGGSTS"
     gene            complement(18202..18990)
                     /locus_tag="ACHLAG_001460"
     CDS             complement(18202..18990)
                     /locus_tag="ACHLAG_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654720.1"
                     /translation="MNVSFTEQVQRNMPQWANEPLRAWNAAEFAAMSDFITEHYWTGQ
                     GSINVFRIVGTDHPQYAGMSWLELLERGKRMDINIPLVEKNPGYYTQAEQLHAGMSFV
                     STDGIHWYVSADGNHRSCLARFLFHLQGEERTQLHNVAQSIYHADRAFWSACREIHNL
                     ADVLSRHGVYLRLQTRRQCVSREDLACWKVDRFSTEAQLTVDDGRAGGHASAVYKVLL
                     LSAEDAWREVRKLQNRAEALSASPETDIPRSWWLRLLQRGRGHE"
     gene            complement(19004..19603)
                     /locus_tag="ACHLAG_001461"
     CDS             complement(19004..19603)
                     /locus_tag="ACHLAG_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP0654721.1"
                     /translation="MLRQMTDIVPDKIPYGVLLCVLTVLFLLFPVLCGRRRRRERASE
                     WRHRHYRRKAERVLAQLSSLPEGGAGVKYLRKINPYVFEELLLLAFERRGMRVIRNSR
                     YSGDGGLDGQVLIDGERWLIQAKRYGRTIRPSHIDDFGELLRREGCRGFFVHTGRTGP
                     ASRARLSLWPDIELISGQRLLKLLNGRLLTGELTGEKRR"
     gene            complement(19603..20109)
                     /locus_tag="ACHLAG_001462"
     CDS             complement(19603..20109)
                     /locus_tag="ACHLAG_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFP0654722.1"
                     /translation="MTAVWPLLLCAAQAGAELRVIADIGGSDASVFYEAINRQEGSST
                     PPPVTAPLPAQGETLALPVTTPEMTPGEVTPRPLQLPGIGALFLVGDDALSRQWLQAN
                     AAALRERQAVGMVVSVADENSLRTLRELAPGVMMVPSSGSALARRLQLNHWPVLITDS
                     GISGVVTP"
     gene            complement(20130..20792)
                     /locus_tag="ACHLAG_001463"
     CDS             complement(20130..20792)
                     /locus_tag="ACHLAG_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase"
                     /protein_id="HFP0654723.1"
                     /translation="MARGSLLTMALLSMAVSTLCRAGAHDIPEGYVRVAAVHGVPAEV
                     LYAVSLTETVMAPSAIAGVMKKRDADFRLPSVARPWPWTINVAGKGYRYASRLEAWQA
                     LQVFLTRYPARRIDVGIAQVNLGWNGHRFSSTWAAFDPYISLNTAATILSECRRRHPV
                     SWLQAAGCYHHPAGGAPAVRYTAVVRQHIERLHRDAVKTAEQHDGLTAMHPRYIWTEP
                     RE"
     gene            complement(20771..21493)
                     /locus_tag="ACHLAG_001464"
     CDS             complement(20771..21493)
                     /locus_tag="ACHLAG_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFP0654724.1"
                     /translation="MKMKNLSPVLMLLISLQTVAAVRESAPEGTRTQASSQVSTTTQA
                     LQQQAAQWGVTEEDYRRYRALMEGPRGIQSPGLDPLTVLGIEARTDAERRQLAEKWVK
                     AEYERTEKELAFQREVNAAWKRLYPGVMPVSLGKGGGLLNDNGGRLALFVKAKDCGTC
                     DARLASVLSSGRQVDIYLVDSQGSDNLLRQWALEHDIPVDRVRSRHITLNHDAGRWLR
                     FGEGRMPVVLQQGADGWRVAAF"
     gene            complement(21498..22265)
                     /locus_tag="ACHLAG_001465"
     CDS             complement(21498..22265)
                     /locus_tag="ACHLAG_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654725.1"
                     /translation="MSEKKQKTLPSVHSSPLDGWTCVSPMLSVGFVLTVLVATILAVC
                     GLYRYSGEQDERIAALEGQMKTYSDAFALSGSELEPLRSRLAETEKQLKSVTERLTLL
                     EQRPVLTEEEHARTGQMAASLASLQATLAQQGETLRAYDRRLEKLEKAPFTPVSPSSA
                     KGTGTVKSTPAVTPRQKNGSSGVSVISRSAPFVLTGTEQRGGRTYAAVAPRGFTRLSD
                     VTLLGEGESTAGWMLERAGRGEASFRVNGRQVRVMVE"
     gene            complement(22262..22840)
                     /locus_tag="ACHLAG_001466"
     CDS             complement(22262..22840)
                     /locus_tag="ACHLAG_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HFP0654726.1"
                     /translation="MKTSLLLSVLSACVLLTGCQGQQKLLQRDAAVPVPSVTVSNNVR
                     PASPDMYAGTTVPEVVRYDRYVLVNTHPDAVQRDPLSQVIDIRIPASLKPTVADAMRY
                     ALKQSGYTLCATGPANGVLYRQALPAVQYQTGPLRLRTALQMMAGPAWQLEVDDVQRV
                     VCHSLREGYQLPPGQLAPVPAGVRDAGAGLKK"
     gene            complement(23031..23384)
                     /locus_tag="ACHLAG_001467"
     CDS             complement(23031..23384)
                     /locus_tag="ACHLAG_001467"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0654727.1"
                     /translation="MTDIELTGKSITLTDKYGSSVHLNTVLTYLLELYQMDFAELSEA
                     VSTHYPSHKGVIMTIAEQLEEKGLEKGRAEERKKALEATYASVRRMSDMGMSTEVIKQ
                     ALQLSDEQIREALHN"
     gene            complement(23457..24392)
                     /locus_tag="ACHLAG_001468"
     CDS             complement(23457..24392)
                     /locus_tag="ACHLAG_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP0654728.1"
                     /translation="MSEKVNKTSLIHDAAFRKTLKDPAAARDFLEQVLTPYQKSRCNL
                     DTIELEPTTFVAESLRQSACDVLLSMKTNDGKDGYIYTLIEHQSSPDKFIPLRMMRYI
                     LAVMEQHIEEHKCAPVVIPVLFYHGAKRPYPYPMNWVDCLDDPVYGREIYGEQKPFSL
                     VDVSTLTDDEIEHYHRMAALMFTMKSGTSGDVIELIGKSITLTDKYGSSVHQNIVLTY
                     LMELYQMDFAELSEAVSTHYPSHKGVIMTIAEQLEEKGLEKGRAEERSRLVLMMRQKG
                     KSLEEIKDFLDLTDEQLLQALDYVPVQRNGALNQD"
     gene            complement(24439..24996)
                     /gene="ssb"
                     /locus_tag="ACHLAG_001469"
     CDS             complement(24439..24996)
                     /gene="ssb"
                     /locus_tag="ACHLAG_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0654729.1"
                     /translation="MASRGVNKVILLGHLGQDPEVRYFPNGGGAVANLSLATSDTWKD
                     KQSGEMKEVTEWHRIVIYGKLAEIAGEYLRKGSKVYIEGQLKTRKWFDQQNGVDRYST
                     EVVVGVNGVMQMLGSLRQQGTAGNGASAPAQGGWGQPPKTAGQAGRAPQTTSTGNGAP
                     PMDFDDDIPFMGLGYGMERRIIHAM"
     gene            complement(25009..25212)
                     /locus_tag="ACHLAG_001470"
     CDS             complement(25009..25212)
                     /locus_tag="ACHLAG_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654730.1"
                     /translation="MNRQNGILAAAAGYTLPLEILKSTRGYYIGTQCSEGPVSRESEE
                     YFKKHDQAEQALKNGTWTQRCDW"
     gene            complement(25306..25770)
                     /locus_tag="ACHLAG_001471"
     CDS             complement(25306..25770)
                     /locus_tag="ACHLAG_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFP0654731.1"
                     /translation="MSATNTSSAKAEYFNLTVKGLGYLSNVRQVNHQNGSFLCCVINA
                     LSGPTDNPSYTRFDVTVVGKDATTLISRCQKAVDEDRKVLLGFALSNLATDIFTLNKG
                     EHAGEQRVSLKARLIKVDWIKIGQEQVYKAEKAESLPPQNGTTKRQYAENSF"
     gene            complement(26416..28425)
                     /locus_tag="ACHLAG_001472"
     CDS             complement(26416..28425)
                     /locus_tag="ACHLAG_001472"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFP0654732.1"
                     /translation="MRLYLCEKPSQASDIARVLGAARRGEGLIEGNDFVVTWAFGHLL
                     EQAPPEAYGEQYGSPWSIEVLPVIPEEWQMVVKDKIARQFNVIRRLLGKADEVVIATD
                     ADREGEIIARELLDYCGYTGVVRRLWLSALDESSIREALGRILPGEKTARLYEAGKGR
                     SRADWLTGINLTRLYTMKARECGVSGVLSIGRVQTPTLAMVVRRDAEIKNFIPVPYWQ
                     LNLWLEKDNVRFRAVWVAAEQYCDAEKRCVNVQAATSVQSQCRQAGVAVVREVSSKRE
                     KTPPPLCFSLGTLQEVCSKKFGLGAQAVLDIAQSLYEKHKATTYPRTDCGYLPVSMHK
                     EVPEVLAAVANTDPDIAPELAKLSPDIVSRVWNDKKITAHHAIIPTRQAFNIGALSDN
                     ELRVYRLIRQHYLAQFLPVQEADVTEATFVVAGQLFGTRGRVEVVKGWRVFIADAPHD
                     EDEGGDTEQTMPLPVLAKGDECRLVEAELKEMKTTPPRHYTEGTLIAAMKNAAGMVSD
                     PALKKVLRENAGLGTEATRAEILETLFTRKYIEKQGKHIRATQLGAELIAGLPEVLTS
                     PGTTALWEQALDDIAQGKLSLASFMEKQKQWLNHLVSGARRQQLQITAPVTPVCPLCQ
                     GRMRKLKGKNGEFWSCQRYPECKGVISEQKTSHSKRGGRKIKKRG"
     gene            complement(28438..29118)
                     /locus_tag="ACHLAG_001473"
     CDS             complement(28438..29118)
                     /locus_tag="ACHLAG_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFP0654733.1"
                     /translation="MTDEKKAEPGVLKSTLSINLHTYYAIRLWEGRKPEPGADKSERR
                     RRIIGMPGVISRAKIASKDSENDNPWADMFLFNLEEKLSKSKQEIDLLVEQLNEVLRN
                     VPGDIIISGVASTSPVNIGVHSRSPLGYKSVWLLVGYDELVLKAFQAFHYGLIARARR
                     DELLNAGGHAVRQICALAQSYKTVPATRSDISSGSQKGKEAISRYGMPDPDVLSGKKR
                     SSFSAPLK"
     gene            29262..29747
                     /locus_tag="ACHLAG_001474"
     CDS             29262..29747
                     /locus_tag="ACHLAG_001474"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654734.1"
                     /translation="MQVVSYTVVAVPPSLVFCTVVAVPPSLVFCTVVAVPPLLIFYTV
                     VAVPPSLIFCTVVAVPPSLIFCTVVAVPPLLIFYTAVPVAPSIIFCTVVAVPPSLVFC
                     TVVPVPPSLVFCTVVAVPPSLIFCTVVAVPSSLIFYTVVAVAPLLIFCTTVTVPPSLN
                     L"
     gene            complement(29756..31042)
                     /locus_tag="ACHLAG_001475"
     CDS             complement(29756..31042)
                     /locus_tag="ACHLAG_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFP0654735.1"
                     /translation="MALPAESLIAHTLRKMKASMAEKASREEGRIRSGLLFTGNVHDA
                     VPRRLLLDTRLSPLDKTAWMMIRLHALSNEGAVFPTYDELQLQLASPGSGKASRETVS
                     RVLTMLRLTGWLSLCKRVRDEKGRIRGNIYALHDEPLGCCDAEKFDPRYLDMVLDSAC
                     KNRNKTIRYTAREVLSDIKNEPGMRHYHSHITLFESRMGAAQTPSQMAARQRVNPAVN
                     GRAAKSGSESAQPPVCASVTELSAQVTELCTAVTEPGEKTEVTQRSTDSEQPGKSVGY
                     HSVRKPNHYVRNITHSMKKNTYVLPDALKSQLSVEDTGMLTKQLQALPETLAEGVLDS
                     LSQMLSKNTLQNPVGWMLAVMKRAREGNFNSPDMSRSGTIQSQPARPSSQSGHGCDER
                     ERRRREVASPEKARNIVAEFRASIQRANAARLAMRG"
     gene            complement(31306..31533)
                     /locus_tag="ACHLAG_001476"
     CDS             complement(31306..31533)
                     /locus_tag="ACHLAG_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654736.1"
                     /translation="MKLKMQTISGPVIIDSNEVTQFYPDHESGGELTAVEYFTDEGRC
                     TTRVRHSFYQVAEALASAWHVDDASKRNRRG"
     gene            complement(31530..31745)
                     /locus_tag="ACHLAG_001477"
     CDS             complement(31530..31745)
                     /locus_tag="ACHLAG_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654737.1"
                     /translation="MRKISGMLNLEKARMQLRVLLSGPVPAITPMAHAVAIKVVLDAL
                     DASERRVAELEQVCTAAGIRIKGEQVK"
     gene            complement(32036..32164)
                     /locus_tag="ACHLAG_001478"
                     /pseudo
     CDS             complement(32036..32164)
                     /locus_tag="ACHLAG_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32498..32676)
                     /locus_tag="ACHLAG_001479"
                     /pseudo
     CDS             complement(32498..32676)
                     /locus_tag="ACHLAG_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(32678..33412)
                     /locus_tag="ACHLAG_001480"
     CDS             complement(32678..33412)
                     /locus_tag="ACHLAG_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP0654738.1"
                     /translation="MNKIDYQALRKAAQNYQSTLAWYQSIPDSPNAERDCDAALAAFK
                     HHIRHREVDIISGLLDELDEKQQYIKSRDQENEDIALTVGKLRVELEEVKQHAEELSE
                     TNAVRNQWRPDICPITGRAFFMWIEHPTLGNVPTYGGPLDSYTIPTKDGDGEFSCERY
                     DHDFGGWVESECPGLYLIDDKEQCRVYELEERVKELDAREISLPERSSMLHRTDFNEA
                     YHTVMAYKVSEVIAAIRVAGIRIKGE"
     gene            complement(33399..33656)
                     /locus_tag="ACHLAG_001481"
     CDS             complement(33399..33656)
                     /locus_tag="ACHLAG_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654739.1"
                     /translation="MSNAARLQLGFSPFSKTIVLAKMRNLDDGTSLRVGNDPGRDVTN
                     EAAQLVWHLVMAEGGEIMWEMDDGSRMVLKAESQEATSEQD"
     gene            complement(33666..33914)
                     /locus_tag="ACHLAG_001482"
     CDS             complement(33666..33914)
                     /locus_tag="ACHLAG_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654740.1"
                     /translation="MKMTELVPGVLTSPGVPPASQRKAVELVNRFRRGERNYHELKER
                     GFGYFKIDVGPDWRLLSRNKGLTWALLSHERYNNMRLK"
     gene            complement(33911..34489)
                     /locus_tag="ACHLAG_001483"
     CDS             complement(33911..34489)
                     /locus_tag="ACHLAG_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFP0654741.1"
                     /translation="MSNNLSQATNGLLMQLVMDLKSGYLRRCEALGLSREEMQMLQGL
                     SIEEIHYLSNSEVSVLRLDINHNNLVRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRISGIDVRPGRGITLSDDENSELWRLWQKAGINDVESVDGLDVMML
                     CAEQMNIPLTAIWHAVRGWHTTGNPDQVRSAS"
     gene            complement(34486..35217)
                     /locus_tag="ACHLAG_001484"
     CDS             complement(34486..35217)
                     /locus_tag="ACHLAG_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFP0654742.1"
                     /translation="MNADAARQDQLIKRIRKLLALSRNNSNPHEAGQALSRAQALMRK
                     HGITELDTDISVIKTSSSKGAPSEAKKVPVWMGRLMHVVAEAFGCHTYLDWRITTGRY
                     WRRVVIFYGFGERPVVAAYAFDVLCRQMQKATKAYLSTQSRTLKLSTRRARAAMFRDG
                     WVYGVRDVVDAFPLTEDETRVMKTWLKRQQFEGMAAFRNVKDCNGGDRARWQGYSEGK
                     KATLHHGVGSRNLRPESLISGGGEV"
     gene            complement(35207..36832)
                     /locus_tag="ACHLAG_001485"
     CDS             complement(35207..36832)
                     /locus_tag="ACHLAG_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFP0654743.1"
                     /translation="MSIKRNHSLDLGAAIMQPGRQASKTGNVVALGETPMVLTLDQLR
                     PNPDNPRTTRNPRYDDIKSSIFSRGLDTVPKVTRIPESEPDIYIFSDGGNTRYQILRE
                     LWEETGEQRFYRIHVLFKPWPGRLQCVIGHLAENEVRGELTFIEKAQGIHKARMIYEE
                     SLQRTVTIRELASLLTDEGLPVNHSSVSRMEHALKYLYPWIPDLLESGMGSPQVRALL
                     SLRQDAERIWGQFAVAAENDVDFDRVFGECCGKFNSPELWSMEMFRDELIGDLLKALP
                     HPVLDYDRWLLELDPRERNRRHHFGEPEPVLMPADSPPEPGAQVASGGATEVAQVVAA
                     TPADRESVMDTAQSGAEKEDRPVADKCERRRKEVQPDMYGGEDSYSGDCGEDATGAST
                     DIPIMPEDNAMQEGGEFRQASAFTGAEQDDIESLQNEAFHLAWELASHTGCVDEIAMD
                     RESELSAGFGAAEANCSPVMAFLVRLTGEEVLRVNPVSLDALLIGGSTEEDLPLLDDE
                     HAGKLLRLLGVLRRLRGLQRMVEDESEEGRDEC"
     gene            complement(36822..>37649)
                     /locus_tag="ACHLAG_001486"
     CDS             complement(36822..>37649)
                     /locus_tag="ACHLAG_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFP0654744.1"
                     /translation="DELDEMTCGLQPGDLILLAARPSMGKTALGLTLCLNALRERTDE
                     TVFLFSIEMSREQLILRTLSMLSRVELTRLRSSMMDDEEWARLTATMGEFMQEGTFAG
                     EGNRLIIDDTSSQTPSSLRASARRYVRLYGRPSLIMVDYLQLIRSPDQENRTQEIAEI
                     SRALKSLGKELGCPVLALSQLNRQVEQRADKRPNNGDLRDSGALEQDADLIMFIYRDE
                     VYNPGTLDKGVAEIIVSKQRQGPTGTIRVSFDGRYTLFSPFERGWNFDRSGGNAYVN"
BASE COUNT         8566 a        11655 c         8827 g         8601 t
ORIGIN      
        1 gttttagcaa taccttctgc aatgatcgtt ttattctttc acttttatca aaacatcacc
       61 agaaacagga aatgactgtc gcaataatga acataaaaag acaaaccagg atggcagtaa
      121 atcaataaag tacgatttca taagaatcat cagaaaaacg gtggacgcga tatccggcac
      181 gaggcaaaac acagcaacaa aaaataaacc ggatatcgcg cccggtactt ttctaactca
      241 acttcaggca ttgtgcaagt tttgatcgtc ggtggattca cagtcatgcc ccattgattc
      301 aggctgaaga ctcatatatc attggaagag actagaatct cattgctgtg aaattcctta
      361 cagaatcttc agccccattt tatggggctt tttttggtct gaacccaggc agttaccaca
      421 ccttcggtat gtgaataaaa aagcccgcag tttttacgct gcgggctttg ttttttcttt
      481 gtcacttgca tgcacgggtg cggctttcga ccgaaccttc ggcaaccgga tagcgggggc
      541 tttcaccccc gctgcggttt cttacggttt acactgcaag aacgccgcaa attctgacca
      601 catgatgtta agtctgaatt cacatggtga acgatgtatc atccagacgg tcaacaacat
      661 ggttaagcat aaaatgctca cgatgatcgt tttttgcggc atagcgcttg ctcctttttc
      721 aaagaggcgc taaccttcca cttgctaaag gtatgtagat tagggcctcg gctaaacaga
      781 gatgttttcc ggggcatttc cacatccggc cttcaggtaa tccttccagc cgtcagccga
      841 aaggcacccg cgcgtaatgt actggttttc agagaggtcc gcaatcttct ggtgacggga
      901 tcatctgcac gccccggagg gacgggctac ctcagtatat tcaggtgcag gctctctctg
      961 tccggtccgt tttaaaattg tcaaacgcac ctttgcggcg aaatcagccg ccttttttcg
     1021 catcaacaaa acgcccaaaa cgcccaaaac gcccaaaacg cccaaaacgc tgggtaaagc
     1081 gtgcaacatt accttctgaa gcaacagttc tcagtttgcc tgtatagaac ggatgcgact
     1141 tagaagaaac gtcaattgta acatatgggt aagttacccc atccagttca attacccggt
     1201 cagttttgat cgtcgacccg atcctgaagt actcatcaac acacgtgctt gatgaagtga
     1261 tttcgttact gattgatatc atcgatccac aataaaaata agctaaagta gctggcattg
     1321 acacgattta cgtttcttat catctctctg ccgcttcttt cttccattga acatttataa
     1381 tacagcacgt atgatatcac tgtgataact acctgctcag gggagctgcg ctatgaaaaa
     1441 cgaagcagaa aaggcgatgt ggagttgcaa aacctcggat accccatgga ccttcagttc
     1501 cctgctgaaa aaggctgtct gcgcgatttt gcctgatgca gaaccgaaga ttgagaatgc
     1561 cacaaaagga tgttctgttt tatcgactgt agataatgcc cctggcaata aatcattaaa
     1621 ttacgaccct ttttcttttg aatctggttt acatcttgat atagaaaatc ttccccttat
     1681 agaaaattgg gatggagaac cttacaagct cccctaaaaa tatggcgata tctatcacaa
     1741 atatcgccat aaatattaac aattatttta atttattcgt aatacctcca gcatgttgac
     1801 cagcttgttg gcttgttgac actccttttc cgattgccgc attaatcacc tgtccgatac
     1861 ttaccccagc ccaggataat gcccccagcc acacagctgg cagtacaata aacatcgtcc
     1921 ccatcaccag gttcatgatg atatcgtccg acgtgttctg cagccccatc atattcagac
     1981 tcgaatgtgt atctgaacca tacagcgccg ttatcatcca gctgtccagc caccgcgcca
     2041 gttcccacca gaatgtcaga aaattcagtg caaataccac aaacgtcagc gtgatcagcg
     2101 tactgaattc atatgccgca aaagcaagta tcatcgggag catgatatac accgccatca
     2161 gcagaatact ctgcaccatc ggcagcgcct gccgcatggc atcaaacgcc ggaaacgccc
     2221 ccagactggc gacaccggaa cctgccgttg cgccaatgcg tgaaatggca ttcatcgtgg
     2281 tcatgtctgc attaccacca tatccccgat acacttctcc cccctgagaa atcgtcaggt
     2341 tagccggact gaccagccgt ttgataatct cctcctgata agtcgcctgg ctgttcccca
     2401 gaagcttcat cgctgccgaa agacgcagcc acagcccttc atcaacttcc tccgtgaccc
     2461 gctttttcag ccccacactg ctgtcgttcc accactcccg gcacgttggg tagcctccgc
     2521 gccccgtatc cggacgaccg ctgtcacggg cgtcattcca cggaaacgcc gcccttggca
     2581 ttctggactg cagcgtgccg taatccccct gcatgaacgt gctgctccca atccagctga
     2641 tatccgacag cacctttctg tccttcgtca taccctggtc acgctgtttc cactggtaca
     2701 tcgccagcga gtagcagtca ttggtgaaat cctgcaactc atcggccagc gccctgttcg
     2761 caatgaacgt cctctgcact tcaaaacgca gttgccgcag atcaggcctg cagggaatgg
     2821 acgccacggc cgcctgtgtc acccctttgg aaagcttgtg cacaacaacc caccagatcg
     2881 gggccgccgc cgtcttatct cccaggctgc tgataatccc ctgataaccg gtgtccccgg
     2941 gggctttcgg cgtccacaca ccacaggtct gggcccggct gcgatcaaac ttcagggtat
     3001 ccagcgtcac ctgaaccagc ggtacacagg aagccaccat cacaaaaaac gcaccataca
     3061 atgcattctc gatgcggggt aacgagagca ttcccttgtt gccttcgtcc gccccctctt
     3121 ccctcacctt cagccagata ccgatcactt tgaaagccag aggaagcgca aacagtcccg
     3181 tgccaatcag caaatcccac agaccgttat tgaccagcca gcccagcagg gtcagaaaat
     3241 attccagata gttattggtc gtcactgctc agtcccccgc ggcggaaaaa atcgcgtatt
     3301 cgcacagcag gaaaaacagc gcactgacca tacaaatccg cgcaaaacgc agacggtact
     3361 ctgcccggaa tcccggtgca cgccagattt tccggcatcc ccataccagc ccgcagtaaa
     3421 tacaaaaccg ccagcccagc catccgaaac gggtttcgtg gaaccagagc ctcagcgcat
     3481 ccacctcctg cgcatgacgc atacctgtat ccgcaatcag gagcgctgac gccatgatga
     3541 caatcactgt cagcccccat ttccccagca gtttcatccc tttcacaagc agcccggccc
     3601 ggctcccggg caccatcagc acggttttat tcattttcag gaacctccag tgaacggaca
     3661 cggctgtcag cgttgtcggt ctgctgaatc atcgggttat tactcacgcg ttcattgtca
     3721 cgctcaatga tggtcagcac actgttacgc gccagatccc gtttcagctc catctcactt
     3781 ttcagggcga tgatctccct gtccagagag gcaatacgac gatcgccttc ttccagcgca
     3841 gcaggctgtg cagccgcata cggttcagac attcctgtga gcagcattct gcgcatcacc
     3901 agtgccgttt ccaccgtatc agccatcgcc agttcaccgg ccagacggga ggtcagggac
     3961 atattgtccg gatcgcgacg cagcgcatgt atcaccccgg cagataccgc cagactgccg
     4021 gtcttcagtt ttgccagatt ctctgccgtg ggtttatcct gcccgttcac caggcgaacc
     4081 agctgttccg tatttatgcg tgtcgcctcc tcaagcatcg gcgcaaaccc tgttccggct
     4141 accgccgttc ccggctgctg gtctgagtca ccactggtac attccttcgc atccgtacag
     4201 gtgcgcattg aacgatcacc cagtaccttc accacagcgc tggccgcctc ctctgcattc
     4261 ctgaatttct cacaggcact gccattacag cttcccgcac caacaccgga tgtactggtc
     4321 accggcagac tgttcatcat gttaaacccc gcggccgtca tgtcatgaac cggacggatg
     4381 gcgggctgac cggcaccacc acgctgctga ccaccaatcc atttctgccc ctctttcccc
     4441 gttgcctggc gtccggcgtc atcaccttta acggcatcac cgtcactggt attaacaacc
     4501 tttttgtact cctccatcat cgcggcctgc gtccacttgc tgcgatcgga aaaatccatc
     4561 atccgtttcg ccatgttctg gcagttcagt tgtgccttgt cgaacgccac gtttgcctgt
     4621 aacacaccat tcgtgatcat gtcgtagagg ccgggattcg cacgctgtat caccattgca
     4681 ggaaggctgg agaccgcacc ggtcgccccc tgaatcacat cccccatcag gtttttaaag
     4741 ccgtcagtga tcccgttgag ctggtttcct actgtggttt tcagatcgaa gttcccgcac
     4801 atcagatcgc tgctccagcc caggtccagc ccgagcttct gcatgttact gcgcgtcgcc
     4861 ggctgggaaa taacggagcc accgcccagg gtgtaaaaca gcttgtccga caccgcacca
     4921 tccaccgatt ttccgtaacc aaacgtgctg tcattcagtt ttggcagcga gagaatgctg
     4981 tctgccatga ccggcgtact gcaggacagc atcaccatca tcaccagagt ctttaccttc
     5041 atcgtcatca tccgtctcct taaaaattcg tactgccaag gaaccgctgc ccccgcttct
     5101 tgcagcaact gtagggctgc cagagcgcaa aagcggcatt accatcctcc gcggcctgat
     5161 ggtctccgtc aggaaacacg gcacaggagc gggagacgga gggcgtcagt ctctgccact
     5221 tgtggttacg ggtgcccgta ttttcctcaa ccggatccgg cggccagaaa ccatcctttt
     5281 tcggggtctg tattgattta taaacatgcg gctcaccggt ccggctgatg atgtcagcca
     5341 cccgctgggc aaccacggcc gctgccttgt catcatcggt ctgcatcaca aaacctgaac
     5401 ggggataaac actgccccac aggttttctt cagcctgact gccgatttca cgtttaccgg
     5461 gaacgagggc ttccggatac agggattcag gcattccggt ccgccagacg agactgtcca
     5521 gggtactcag gaaataaggc attgccggtg tcaccggcga atcgcagctg aatcccggca
     5581 ccattccgcc aataagacgc gttgccggat gccccagcgc atcggcatat ttaaaacgaa
     5641 tggccgaacg acgctctccc tgagctttca tgtccgcagg atttccccct ccggcactca
     5701 gccccgccag gcctttcgta atggcattct ccagccctcc ggctgtctga ctgaccagcg
     5761 acatctcttt ccagggattt ccccccggtg ccgtgtaggt ggaaaccacc acttccggaa
     5821 tataatgtgt cactttcacc gatgttctga ccttacagct gaacccggag cagtacagcc
     5881 agtagcagat tccactgacc cgccagctga tacaatccgg cgctgccgca ctggccacaa
     5941 tactggcggt attcagtgaa gctgacgcca gaggcagggc tgcaacactc agcagcaggg
     6001 ccccggcacg cagacgaaat gaccggctca tcatggctgt actccttcct gtgctctcag
     6061 ttgtgcggcc tctgccacat ccgtggtacc gtagaccacc tcacgatcat caaagaccac
     6121 ggccggatat ttcttcacgc ccagtgacca tgcatacgcc acctcccggt aacgctcagc
     6181 cagctccgcc tgcatcgccc cccactccgg ggactgcata cgcgccctga cccttgcttc
     6241 tgcctcagcg gcatccgcgg gcagtgcacc aaaaaaacgc gcctgcagtt gttcagggcc
     6301 atccagcagg atcacccgta cgtccgcagg cggattgacc ggaaggtgtt gagaatcggt
     6361 ataaagggtt atgccggctg acaccgccat cggcaggaaa aacagggaac agaatataat
     6421 ccggtgcatg gtacgcctcc gtcagacaat atctgaatga cagcgtgcgc agaaaacttc
     6481 acgatagcag caataaattc aaaaccggat gatgaaaatt aacgcagaga atggtcagtg
     6541 gaagggcatg agtggaatga tgccgtcgat gaattcttcc ggtgaagacg gataaaaata
     6601 ttgccgtaat gtaataaacg tgttaattaa aaacgcgtgt accggcaggt atgaattgat
     6661 cccgtaaccc atctgccggt tacacgctgg cgcttatttt tctgcctcca taccattcag
     6721 ccacaacagc agatagaaca aatcggccgc atcaacaacc tggatatacc atctcagatg
     6781 cgtatactcc tcctctgacg gccagcattc atgaccacac acggaaacaa gcagatagac
     6841 ctttccggta tcaccttcca gcgtcaccgc cacgctgctg ttcatgccaa cccgctgggg
     6901 ctggcataca acacgacgca gcctgcccag acgacgatta aggtgtgagc gcagccgcag
     6961 aagatgcggc tgtgttatct cggtcacccc gggcttaccc tgtggcggca aaaaaaacac
     7021 aacgccaccc ggatgcaccg ctgtctgcag ctccggctcc ggatatttca gatacatcat
     7081 gccgcactct ccacggcgga aagtccccgc agtctgtcca gtttacgggc cacatggaac
     7141 gcagcctcca gttcgctgca gttatgttcc ttcatcagca tgcgccgctc agccttctcg
     7201 tccttctccg tcatccccag cgccagataa agactcggtg gcaccgcacg gaacagcgcc
     7261 tcaatcttct tcgccaggac aaccccctcc gtataacagc cggacagttt gctcgccgac
     7321 atcagcaccg ccttctgctc ctccgtcagg gatttaaaac gggcaatgtt gttcacctct
     7381 tccggcggca tcgtcagaca cacccaccat tccgccatgt tcagcatttt ctctgatata
     7441 tccggatagt ccttcaggtt ctgcgtcgca agccacagcc aggctcccag cttacgccac
     7501 attttcacca ccttcgtcat atagggcgag agcagcggat tcaccgtcac gatatgcgcc
     7561 tcatccacgg caaacacaat atcacgcccc agatactggt cacgttcggc aatgttattg
     7621 atcatgttgg tcatcgacac catggtcagc gccatctgcg cctcataccc ctcacgggcc
     7681 aggtgcccca ggtctatcag cgtcacatcc gcttccggcc acagttcgcc ttcgcggttg
     7741 aacagctccg cctcaaaact ccctttctgg gtgaacattc ccagtgattc cgccatctct
     7801 gccgcctttg cccggcgtac ctcgttacgt cccgcatcac aggagatttc ccggagtgcc
     7861 acctgcaaat cttccggcag catctgccgc ccttcccggt acgtcgcgtg tgtggccatc
     7921 agcagcgctt cacggatcat cgcccggtcc gcacgtttga gtgaggcctc ttccttcgga
     7981 tcaccgccgg tgatcatcat cctggcagag atttccattt ctcccagaat gtcgcgtttc
     8041 tcctcctctt catcgccatc cagatcgggc agcgagtgct catccacact ctgcagggtc
     8101 tgcccttctt caaccagctt atgcgcatcc gcaaagggcg gcagtgtcac accgctcccc
     8161 ggtttgacac tgattttgtt cactgaaagc ccgagacttt caaagtaatc ggccagcaga
     8221 ccaaacgagt tacccgcctc ggcaataaac aaccggggac gatgcacggc catcacctgt
     8281 gacagagagg cacacagcgt ggcagacttc cctgcccccg tgggcccgaa cagcaacaga
     8341 tgggcattct gggtacgatc cagtttgttc aggggatcaa acgtcaggac atctcccccg
     8401 cggttaaaaa aactgaagcc gggatgccct gtaccggttt cccggccggt caccggcaga
     8461 agccctgcga gatgctgtac ccacatcaga cgtgtgtgcc actgcttcct gtccatatcc
     8521 ggatcaaagc acattggcag cgcacgcagc cagctgttca gcggccccac atcaaactcc
     8581 ggtcgcaccg gctgcaggcc tgcacccagc agaaccgtgg tcagctccac ccgtttacgt
     8641 ttcaggctgt ccatatcacc ggcccgcaga aggaaagtga tccccgcacg atagagttta
     8701 tgccggttac ccagcaactc cttcaccacc ttcacatcct gacgtgcccg gatggactcc
     8761 gtattctcac ccacggcatt tttcgacagc cgggtaaacc gctcctccag ggtgtcctgt
     8821 ggctgtacca caatggtcat gcacagcacc gttccttccg gaaacgtatc catcagcgca
     8881 ttcaccgttt tttctccccg ggacatctca ccggtaatgg cgcccggctc cggtggcgta
     8941 cggagcttct ccaccaccac ggcacaatgt ggcagatcat caatccacca caccccgttc
     9001 tccggatcgg aatgaggcgg ggtaaaccac aggctttctg caaagtccgt catcaccggc
     9061 ataacatccg cagcgttctc acggggatcg gctcttgagg ccatccggta caaatcctcc
     9121 cggctaatcc aggacggtgc cggattaaat aaccggagta accagctgtg aacctgcaga
     9181 ccgttcatcc ggtgacagcg caccccggcg ctccccagtg ccgacacaac gcgatcgcaa
     9241 acctgattca gcatggccac cggcggcatc ggatcactgt tgttcttcgc gatacgacga
     9301 taaatcacca tacgggttcg ccgctgctgt ccccgccacg gctgaccggt aaccagcgtg
     9361 tctgtaaaaa ggccttccgg gcgggcaatt ccccgcaaat ggcgttccat ttcgcccagc
     9421 caggcttccg taaacgccgt tccctgcgcg tgaggtttca catacccttt cagataatca
     9481 agatacgcat ccacgtcatt ttcatcctgg cagaagaact gcaccaccca gggatgcgtg
     9541 tcatactcat caaagctgtc ctgcagtgcg tcctccacca tgtccctgat ctgttcaaga
     9601 cgttcctccg tacgcccttc ggtcgccgcc ggcgtcacgt cataaacagc ccctaccgac
     9661 attccgtcat ccagcagcag acactgtgcc tcatccaggt actccaccca gggaagaaaa
     9721 tcgataactg acggcccggc atgataaacc cggacctcct cttcctgcgt catctgcccg
     9781 ggacgtttca ggggctgcgc cccggccggg acaggcggta caaaaccctg tcctgccgtc
     9841 tccgtttctg atggcgtggt gacattgcga cgtttaaaca gtgtgaatgc cattacatcg
     9901 cctccgtacg ctcaccgggt aacgcatact gcgttctgct gtaaaaagga aataccgtgc
     9961 tgtatcccgg caccggtgac gtcccgccgg caagatgcgg gaatacgtac atcaccatgt
    10021 cagggtttgg cagacgggga aactgctggc tgatttcact ttcctgcgtc ctgctgtagc
    10081 tctgcccctc cctgtgtacc tgctgtttct catctgacgt caggggccgc cacagtgtct
    10141 ccctggcggc cagcgcatgt cgctggctcc cccccgttgt tttcccctgc cagagttcaa
    10201 gcatggtctg atcgcctgcc ggcagaagct cctctttcga ggtggagcac ccggataaca
    10261 gcagggcagt cagggggata cacatcacta accgtaatcg tttcacaaac gtattctccc
    10321 tgttcagtca agccccccgt ggtcgtttgc ttcacccggc agggcgaagt cataacgcac
    10381 acggcggcct tgctcttcat aatcaatagc cagttgtcgg gtaatatgta ccgccagcgc
    10441 tttccccggg ggaacataaa tggcatcaaa cgtctgcccg taacgctgcc tcacccagtc
    10501 tgccgtctca ttcattccac cggacagcgc cttaccaagt acagcctgcc ccgcgctgcc
    10561 ggtcagcgtg gaggtgatac cgttgacatt gttctgcgag gtatactgcc cctcactcag
    10621 ggcatccccg gctgcaccgg cggctgacag tgcaaatatc gttggcagat aactcgacgc
    10681 attggattta cgggtaccgc tgatgcaggg aataccgtct tcatcggaga tccagccaat
    10741 cccgccagta ccattcccct tcgcctgctg attgctgttg gtggtgccgt tgacatccgg
    10801 tgacggcagc gtgcgcaccg tgccatcagc aaaaacaaag gtgatactgt tcacctgccc
    10861 gcgcacacag gaaagcgtcc agtcaccact ggccgtgccg gagacaatcg ccccttccac
    10921 atccggcagc tcaatgccgt ttgccgtgag gttgtcgcgc ccgaccagga tcttgaacgg
    10981 gtaaggatcc gtcacagtgc cgttgatcgg cacccgcccc agcagggccg tcatcgcacg
    11041 actgcctgtc agcgttgaat tttccggcag cgtgtaaacc gcctgtgtgg tggctcgctg
    11101 ctgttcattg ctgtatccct tcacctgctg ctcatactca gccttctgac gactcagtgc
    11161 gttgtcctca aggaatgagg tcggaaaacg agggcctccc tttgaaccac tgctgctcag
    11221 gggatcgtcc tgagactgat cggccggact tacccacatc agcccgtcag ccccctgcga
    11281 aatcaccacg ccactgccgc cgcccgcatc aagcccgagc cccggcggga tatcgctttc
    11341 ctggcggcga ctgagcgtct ctgcccggct cagttgatct ttcatggcgt caatctgcga
    11401 catcagggaa tgctgctgct cctgcagttc ccgctgcttc tgctcgtagg tcttctgtac
    11461 ccctgccaca gcgtcattca cctgaccgga cacatccgtg ctccgcctgc gcagctgctc
    11521 gttatcctta agaagttgcg agttctgttt ctccagcgtt ctctgattgt cccgcacctg
    11581 gttcatacgg gcgaccagcg tccgtaatgt gtcttccggc gtgtcacctt ccaccccaag
    11641 agcccgtagc tcttctgacg ataatccgga taaggcccgg gcctgctccc cgcggtcagc
    11701 aacattttcc tgcgttttgc agcttttcat cgtgactgca agccccacca gaacaacagc
    11761 gggcaccaca aatttcacca gcgcgttgga cttaatctgc atgcgtcacc tccccgcccc
    11821 tgcgggacgt cacaggctct tccacaaacg ccttctccgg actgcccgcc gtcaccacat
    11881 acagcatcgt ggtatcttcc ggcgtaccgg cagcacccag ccagcgatgc tggaaagtcg
    11941 ccgccacaaa acgcccctga agggcccggg gatcaagcac aatacgcctg cttaccgtac
    12001 tggtgagttt cagggccacc accgtccggc caccggctgc ccatgccgcc accggtgaaa
    12061 cagtcagcgg ctctgacgga taaaccgtgg tgatctgccg gggaagatgt ggcaccaccg
    12121 gtctgatccc cgccaccggt tcaagggtgc gcagtggtgc atacagggac tgtgccgcat
    12181 accgggtcaa cagcaccggc aggggctcag cataacgggg ctccgtgcga cgggcctgag
    12241 tactattatc cgtccccggc ttcccgttgc tgctgcgact tttcacagtc tgtacatcac
    12301 tcccggcagc acggctgacc gtactcacgt ctccgtcata cacgatacgc acgggctccg
    12361 tggttccctt ttcccccgca gtgacatcca gcaggatcat ttccccgctt tccacatcct
    12421 gcagctgaac ccgggtctgc ggaaatgcac tgtctgcctt caggtagacc gcaccaccgg
    12481 tactctggac ccgcagtttt ccattcagcg ccggagggaa gcccacccgc acatttttat
    12541 ccgcaaaaat cacccgctcc tgccccacct tcagcgggat attcagcggt atacgctccc
    12601 acttcatcag ttcatccgcc tgtgccgtca gactcagcac tgacaacgac atcatcatga
    12661 cggcaggacg cagcagactg cacacaatgc cggctttcat tcaccacctc ccttcacggc
    12721 ttccagacgc tgtggcgtgg agctgtagca gtccagcaca agaccaaacg gattacgctc
    12781 cggatcgccc tccatccgca tcactttcat cggatagcgg atcagtgccc gtttgaccgg
    12841 ctctgcatga aaatactcat ccgccaccag gtcgagacgg acaagccagt tgtcccgccc
    12901 ctccaccgtg acagggtcac ggttgtaacg cccgccgtaa ccgtgctcag ggatctcata
    12961 caccacacga acacgatcca gcagttctcc tgcatcctta cgacgacggg catccgtctc
    13021 caggatctcc cggcatgacg gtgtcagata ggccgacagt gcatgtattt tcgccggata
    13081 atccacctcg ccatttttcg gccaggcatt cagttgctga aaaatgtaga acgcaaagga
    13141 atagaccgtt gacggaggaa cttcccacca cggacgcgta ctgccggaac gcagatccgg
    13201 cggattatga atggtcagcc ggtcaggggc cgtcatccac ccgatagtga ccataagaag
    13261 aagtccgcac agcacaccgc tgaacaccct cagcgtcaga atatgctgat cacgatcctt
    13321 cagggcatga cgaaaacggc tcatgattta ccccctctga cacgggtacg gcgcagggac
    13381 cagccccggg cctccctgat aagcccggag cgtcccacgc ccagcgccgc ttttttctcc
    13441 tcaagacgct gccatagcca gttatccggt ttcccccgct tcagacggct gatccatgcc
    13501 cccccggtcc agacaagaac cagcggggtc agcagggcgc cggtgggtat aatcacccac
    13561 ccgaacaccg gcacaaaagg aagtgaccac agcagaccgg tgaagaaccc ggtcagtgca
    13621 gccagcccca gttccggggt ggtgaacccc ctgaacaccg tcggttcggc gttcagacgg
    13681 tcgggcagaa aacgaatggt ctccataacc agctcccact gttgcttaca ggatgatgga
    13741 cgcggattta ccaagcagcc agatgaccgc gaccagcagt accaccccca ccacaataat
    13801 ggccccgaac tgcccccagc ccgtcttgcc gtcccgcacc tctgaaaacg tggtaacggc
    13861 cgcctccgcc actttcacaa aggcagccgc ggcgatcacc agaccaatca gcacaatgcc
    13921 atcctggaaa taacctttga tttgcccgaa aagtccgcca ccaccaccgg acgacggcgg
    13981 ttcaatctgc ggcagatcgg ccagtgccgg tgtcagactg cccagcagaa acgtcaccat
    14041 ggccattccc gccagacgcg ccatcaggcg cagacggctg acagtaagcc cttttttacg
    14101 catagatttc tccttaaaaa gtcacaccat gaatcaaccc gcaaacatcc agatgctggc
    14161 aaccagcagc agaacagtgc gggcagtaaa ccagataaac gcagactccc tgacgtcacc
    14221 gtttcgccag ccagaccaga catccgacag tccccaggca gcccagagaa agagaacggc
    14281 aatcagtaat cccagcaagg acagatgcag cacgttcaga tccacatctc cggatgccac
    14341 cttaaaggcc gcagcctgtg ccgacgtcag tgccatcagg gacgctcccg ccggtattca
    14401 ccggtcacgg cactgccgtt acgtggttgc gcccgggagg gcgtgagata acggtcgatc
    14461 ccctcctgca tggtctgaat gtcagcctga gcacgcggat agtcgaaaaa ataacgcccg
    14521 tcgtggggcg tggcctgaag ggacgccgtt ctggcccgat caagcgccgc acggacctga
    14581 tcaagctggc gcatgaccgt cgccagctca tccttttcag cggcctgaac gggcagaacc
    14641 agggtcacga gaagcatcat cccggcggtc cccgtcagac cggccgtttt ccgggataat
    14701 gcaggaagag aaaacatacg cacctccgtc agaatcaatg ggtgcgtaca gcatgcgtaa
    14761 ggcaggggga gggatcagcg ataaactcta tttgcaaaac tgaaaatttt tacgggagaa
    14821 tacacggggg tggataaagg ggcggtcagc attgccagtt tctgcatcac gctgttccag
    14881 gcctgttcaa ataaattcat tccatcctga atgatttatt cctgtttatt aaaacacggc
    14941 atcactacag tttcccgtta atgacacctc attaacaata aaaagaggaa acacatcagc
    15001 atggaatttc tccgcaccgc tcttccgttc tgggccctgt ttcttctgac ctgggtttgt
    15061 gttgtgttgc aaaatggcat ctgaatcgat catggtttgg catctaaatc gatcacggtt
    15121 gtggaatacg cagttgtgta ttttttcatt tatgaagaac cacgttgccc cccgtggatc
    15181 tatgacgtcg tcaaggccat cacgatgttc attgttttcg tcaacattgt tgttattccg
    15241 gcgctggcat tacccggaca ctgacgcgca ctgaccgtaa gggcgtggct tgtctgccga
    15301 caccctgcca caaaacaagt tttgcatcag ggtgtccggc ctctgtggat tataaatatt
    15361 tcttgaagga tgccattgtc acggtgacag ccatacccag cagcatggcc gccggccata
    15421 tcagcagatt cgggtacacg gccgtcggcc aggaaagata gagaatacag ggaatatata
    15481 tcgccggttt aatggacttt ttcgcatggt gataaacaaa gctggattca tacgcggcac
    15541 cataccggcg cagatcacgt ctgcccagcc cctcaaccac ggccaccagg atcaccatca
    15601 cgaacagtgg cacggacagc gcgagaataa tcaccctgac catcgtcacc accgtcacat
    15661 aaaccgttgc cagcaggtaa tcatgaagat gatatgccag ccactggctc agctctccgc
    15721 tgaactgcga cacactacgc cccgccgccg ttaccgcctg ctgcgactgc agccatgact
    15781 gcataccact ttccacaaac aaccactgat acgccacgga aatccagccc ggtaccgtca
    15841 ctgacggcgt ggacagcagc aggctgcgcg tgaactccgt cgacagccag cccagctctg
    15901 tattcatgac ggcacgactg tgtgacgccc cctcatcagg ccagaaaaac gtcatacctg
    15961 catactcaat cagcagactc agtaacagtg aggagaggag cagccccacc agagcccatg
    16021 gccagcccca caccagattg tacagcaacc catgctgacg gggagggcgg gactgcggac
    16081 tgcgttctgc atcacgacgt gcttccgcca tggtcacctc cctcctccgt cgctatcgcc
    16141 tgtactgaag gtggtgcatt ccaccatcct tcaccggagt gataattctg tcgcatcgcc
    16201 tccgccaccc gggaaatact ctccggcatc agcacatcat ccgtatcacc tgccggcagc
    16261 ggcatccgga tcttccataa ctgtccacct tccagcaggg caaatgcctg ccccttgggc
    16321 agcgtgacaa tatctgcggg ctccagcagg gggactttca ccgtcccgac gcgatcctgg
    16381 gtactggagg tgaaatcctg accggctgac acatctgccg tatcctgatg accggagacc
    16441 agcgtcttcg tgtaaatctc cacctggggc agttgtgtgg tcagcaattc cgccgtccgg
    16501 ttctccctga cgcgcagcat gatcatgttg ttaaagttcc cctgaacctg cgccgttttg
    16561 gccgcattac caatacgcgc ttcaatatcc gatgacgtct gggtgtacgc ggtcacctgc
    16621 attccggcgc cgcccccctt gttgatgagc ggaatgaatt catcgcccat cagttcgtta
    16681 aactcatcac agtgcaggtt caccacattt ttacccgccc cgccagccgg cagaccggca
    16741 tgcataccgt gtttgtatat atgccctgcc acactcacca ggtccgcaaa catcgaattt
    16801 cccacggcac tggccacctc actgtcactg agcgcatcga ggccgatata aaccaccgcc
    16861 tttttgcgga tcacctgttc ccagtcaaaa atggggcgcg catcgctcat atccagatag
    16921 tccggcgcca gcagctctgc aatcctgccg gtggtgagtt tttccagtaa cggcagcagg
    16981 gaagccacga ttttgtcaaa atacgtacgg tcatagcgga cagcactgcg cagtccatcc
    17041 agaatcggat cgtatatttt tttaccttcc tcactgctca gtgccatttc aatcgaccag
    17101 atccgcaccg catccggctg gttctgcatg gtcctgggca catcgtcctc gctgagaaca
    17161 ctctggttat tcgcgataat ctgctccagt ccgggcagac gctcacggat caccttcccc
    17221 gcatagcgaa tgtagaggtc agcgatgttg ttcacatacc gcataatcag catatagtct
    17281 ggacgctcac cgagggccac cagggcgcgg gcaatgatat tgacaaagcg ccaggcaaac
    17341 tcccggaaag ccgccgaatt accttcaccg gacaactgac cggcaacacg ggaagccact
    17401 tccgatacac gaccaaagcg tcccaccgca ttgtaacggg ctgaaatttc aggccagcca
    17461 aggtgaaaga tatacaactc atccccccgc ccggcccggt gcgcttcagc ccagacccgc
    17521 agcagcagat ctgcatcccc cttgggatca aaaacgatgg tcacttcacc ccgccggata
    17581 tcctgggtga ccagcaactc tgccagacgg gttttaccca cacgcgtggt gccgtagaca
    17641 atggtgtgcc ccacacgctc acccagtgcc agtgtcacat cctgctcatc aggttcaata
    17701 ccgtgcaggg ccggattccc gcccaccggt ggcaggggac gaaccgggtt cagcggcgaa
    17761 tcacgacgaa gccactgccc cagcccgggc agccggtgtt ccgttttcat ttcaagccag
    17821 cgcgcagccc ggtaaagtcc tccgggctgc agataatgcg ccacctccgg ccgcagcgta
    17881 tcctgcagac gctgggtatg cttctgtgtc cagcgaaatc cccgccccag aaacagtcgc
    17941 tgacggctga caggaatttg ccgcgtgctc atcacatagc ggggtaaccg acgcagattg
    18001 cgtcggtagc ggatgatttt catgccctgg cggaaacgga cagccgccag aacgccaaat
    18061 ccggcagctg tgacataact gaccgaaggc gcaagcgcca ccgcccaggg ggctgtcaca
    18121 cagataccgg ccgccgttgc cgcaaccacg gccgtattga actccacagc tggcctcagt
    18181 aacgcctcaa taacataacg gttactcatg tccgcgtcct cgctgtaaaa ggcgcagcca
    18241 ccatgagcgg gggatatctg tctccggtga cgctgacaac gcctcagctc tgttctgtaa
    18301 tttcctcacc tcccgccatg cgtcttctgc actcagtaac aataccttat acaccgcaga
    18361 cgcatgtccg ccagcccgtc cgtcatccac tgtcaactga gcttctgtgc tgaaacggtc
    18421 cactttccag caggccaggt cttcacggga aacacactgc cgccgggtct gcagacgaag
    18481 atacacacca tgccgggaga gaacatcagc caggttgtga atttcccgac aggcagacca
    18541 gaacgcccgg tctgcgtgat aaatgctctg ggccacgttg tgaagctgtg tgcgctcctc
    18601 tccctgcagg tgaaacagaa agcgggccag acaactgcgg tgattaccat cagcactgac
    18661 ataccagtga atgccatccg tgctgacaaa actcatcccg gcatgcagtt gttctgcctg
    18721 cgtgtaatac cccgggttct tctccaccaa cgggatattg atatccatac gcttaccgcg
    18781 ctccagcaac tccagccagc tcattccggc atactgtgga tggtcagttc ccacgatgcg
    18841 gaacacattg atgctgccct gccccgtcca gtagtgttcc gtaataaaat ccgacattgc
    18901 cgcaaactct gccgcattcc atgcccgtaa aggttcattc gcccattgcg gcatattcct
    18961 ctgcacctgt tcagtaaatg aaacgttcat ccccgctctc ctgtcatctt cgcttttctc
    19021 cggtgagctc accggtcagt aatcgcccgt tcaacagttt cagcagacgc tgtccgctga
    19081 tcagttcaat atccggccac agcgaaagcc gtgcccggct ggccggtccc gtacgcccgg
    19141 tgtgaacaaa aaatccacga caaccttcac gacgcagtaa ttcaccaaaa tcatcgatat
    19201 gtgacggtcg gatggtgcgg ccatagcgtt tggcctgaat cagccaccgc tcgccgtcaa
    19261 tcagcacctg tccgtccagc cctccgtcgc cgctgtagcg ggaattgcgg ataacccgca
    19321 ttcccctgcg ttcaaacgcc agaagcagca gttcttcaaa cacatacggg ttaatttttc
    19381 gcaggtattt cacgcctgca ccgccctccg gcaacgacga cagttgcgcc agcacacgct
    19441 cagccttgcg ccggtaatgg cggtgacgcc actcactggc gcgttcacga cgccggcggc
    19501 gaccacacag gacggggaaa agcaggaaca acacggtcag gacacacagc agaacgccgt
    19561 acggtatctt atccggcaca atatccgtca tttgccgcag catcacggtg tcaccacccc
    19621 ggatattccg ctgtccgtta tcagtaccgg ccagtgattc agctgcagac ggcgtgccag
    19681 tgctgacccc gatgatggta ccatcatcac gccgggcgcc agctcacgca gcgttctcag
    19741 gctgttttca tccgccacgc tgacaaccat accgacagcc tgccgctcac ggagagcggc
    19801 cgcattcgcc tgcagccact gacgggaaag cgcatcgtct cccaccagaa acagcgcccc
    19861 gatccccggc aactgcagtg gtcgcggcgt cacctcaccc ggcgtcattt ccggcgtggt
    19921 cacgggcagc gccagcgtct ctccctgcgc cggcagaggc gccgtaaccg ggggaggcgt
    19981 actgctcccc tcctggcggt tgattgcctc ataaaacacg gaggcatcac tgccgccaat
    20041 gtccgcaata accctgagtt ccgcgccagc ctgagccgcg cacagcagca ggggccacac
    20101 tgccgtcatt acatatcgtt tcactggtat cactcccggg gttctgtcca gatataacgg
    20161 ggatgcatgg cggtcaggcc gtcatgctgc tctgcggtct tcaccgcatc acgatgaaga
    20221 cgctcaatat gttgtctgac cactgcggtg tagcgtactg ccggagcacc acctgccgga
    20281 tgatgataac aaccggccgc ctgcagccag ctgacaggat gacgacgacg gcattcactc
    20341 aggatggtcg ctgcggtgtt cagactgatg tacggatcaa atgccgccca tgtggaggag
    20401 aaacggtgcc cgttccagcc cagattgacc tgagcaattc ccacgtcaat ccgccgggcc
    20461 ggataccgtg tcagaaaaac ctgaagtgcc tgccaggctt ccaggcgact ggcatagcgg
    20521 tagcccttac ccgccacatt gatggtccac ggccagggac gtgcaacgga cggaaggcgg
    20581 aaatccgcat cccgtttttt catcacaccg gcaatcgcag agggcgccat caccgtctct
    20641 gtcagagaga cggcatagag cacttctgca ggcacaccat gcacggcagc cacgcgcaca
    20701 tacccctcag ggatatcatg cgcccccgcc cggcagagag tgctgacagc catgctcaac
    20761 agcgccattg tcagaaggct gccacgcgcc atccgtccgc tccctgctgc agtacaaccg
    20821 gcattctgcc ttcaccaaaa cgcagccagc gaccggcatc atgattcagg gtaatatggc
    20881 ggcttctcac ccggtccaca gggatatcgt gctccagtgc ccactgccgc agcagattgt
    20941 cactgccctg actgtcaacc agataaatat ccacctgccg ccccgaagac aacactgatg
    21001 ccagtcgtgc atcacaggtt ccacagtctt tcgctttcac aaacaacgcc agccgcccgc
    21061 cgttgtcgtt tagcagcccg ccccctttcc ccaggctgac cggcataacg cccggataaa
    21121 ggcgtttcca ggccgcattg acttcccgct gaaatgccag ttccttctca gtccgctcat
    21181 attcggcctt cacccatttt tcagccagct gccggcgttc cgcatccgtc cgtgcttcaa
    21241 tccccagcac cgtcagcgga tccagaccgg gggactgaat cccccgcggt ccctccatca
    21301 gagcccggta tcgacggtaa tcctcctcag tgactcccca ctgagcggcc tgttgctgca
    21361 gcgcctgtgt ggtggtactg acctgcgaag aggcctgcgt gcgtgtgccc tcaggcgcac
    21421 tctcccgtac ggcagccacc gtctgcaggg aaatgagcag catgagcacc ggtgacagat
    21481 tcttcatttt catttcatca ctccaccatg acccggacct gacgtccgtt cacccggaac
    21541 gaggcttctc cccgtccggc acgctcaagc atccagccgg ccgtgctctc cccttcaccc
    21601 agcagcgtca catctgaaag acgggtaaat ccacggggag ccaccgcagc atatgtgcgt
    21661 ccgccacgct gctccgtgcc tgtcagcaca aacggtgcgc tgcggctgat aactgaaacg
    21721 ccggaagagc catttttctg gcgtggcgtg accgccggtg tgctcttcac cgtgcccgtt
    21781 cctttcgcag atgacggtga taccggggtg aatggcgcct tctccagctt ctccagtcgc
    21841 cggtcatacg cacgaagcgt ttctccctgc tgcgccagcg tggcctgcag tgatgccagg
    21901 ctggcggcca tctgaccagt gcgtgcatgc tcttcctccg tcagtacagg tcgttgctcc
    21961 agcaatgtca gtcgctccgt cacgcttttc agctgttttt cggtttcagc cagccgggac
    22021 ctgagcggtt ccaactcact tcccgacaac gcgaaagcat ctgaatacgt tttcatttgc
    22081 ccctccagtg cggcaatgcg ttcatcctgc tccccgctgt aacgatagag gccgcaaacc
    22141 gccagaatgg tcgccaccag aacggtcagt acaaatccca cggaaagcat gggtgacaca
    22201 caggtccacc catcaagagg agatgaatgc actgacggta atgtcttctg ttttttttca
    22261 ctcatttttt aagccccgct cctgcgtccc tgacaccggc cggtaccggc gccagctggc
    22321 ccggcggcaa ctgataacct tcccgcaggc tgtgacagac aacccgctgc acatcatcaa
    22381 cttcaagttg ccacgccgga ccggccatca tctgcagggc ggtacgcaac ctgagcggcc
    22441 ccgtctgata ctgcactgcc ggcaacgcct gacgataaag caccccgttg gccggtccgg
    22501 tggcacagag cgtgtagccc gactgtttca gggcataacg catcgcatct gccaccgtcg
    22561 gcttcaggga tgccggaata cggatgtcaa tcacctggga aaggggatcc cgctgtaccg
    22621 catccggatg agtgttaacc agcacatagc ggtcataacg caccacctcg ggcacggtcg
    22681 tccccgcgta catatcaggg ctggccggac ggacattatt gctgaccgtc accgacggta
    22741 ccggaacagc cgcatcccgc tgcagcagtt tttgctgccc ctgacagccg gtcagaagta
    22801 cgcaggcgga cagcacggaa agaagaaggg atgttttcat caggaagtta cctctgcggt
    22861 gaagtgataa agtcagaggt caatttgcag aagaagtcca gaccggctca atgaacaact
    22921 gatttccggt tgacaaaaaa aacgcctgca caatagcagg cgctttgttt acagggcata
    22981 acgcattctg attcatcagt cgcgctttgc cacatcatta tgatgaaaag ttaattatga
    23041 agagcttccc gaatctgctc atcagaaagc tgaagtgcct gcttaatgac ttctgtcgac
    23101 atccccatat cactcatgcg gcggacagat gcataagtgg cttcaagcgc ttttttgcgc
    23161 tcttctgccc gacctttctc cagacctttc tcttccagtt gctctgctat cgtcataatc
    23221 acccccttgt ggctgggata gtgtgtggat actgcttcag aaagttcagc aaaatccatc
    23281 tggtaaagtt caagcagata agtgagcacc gtgttcaggt gaacgctgct gccatactta
    23341 tccgtcagtg tgattgattt accagtcagc tcaatatctg tcactgccag aaactaccaa
    23401 aaaaacgcct gcacaaggca ggcgtggtgt aaaagggaac ggagtcttcc ggttattcag
    23461 tcctggttta gcgcaccatt acgctgcacc gggacgtaat ccagggcctg cagcaactgc
    23521 tcatctgtca gatccagaaa atctttgatt tcctccagag attttccctt ttgtctcatc
    23581 atgagcacta atctggagcg ctcttcagca cgacctttct ccagaccttt ctcttccagt
    23641 tgctctgcta tcgtcataat cacccccttg tggctgggat agtgtgtgga tactgcttca
    23701 gaaagttcag caaaatccat ctggtaaagt tccatcagat aagtcagcac gatattctgg
    23761 tgaacgctgc tgccatactt atccgtcagt gtgattgatt taccaatcag ctcaatcaca
    23821 tcaccactgg tgccggactt catcgtgaac atcagcgccg ccatccggtg atagtgttca
    23881 atttcatcat cggtaagcgt gctgacatcc accaggctga atggcttttg ctcgccataa
    23941 atctcgcgtc cgtacaccgg gtcatccaga caatccaccc agttcatcgg atacggatat
    24001 ggccgctttg caccatgata aaacagcacc gggatcacta ctggcgcaca cttatgctct
    24061 tcaatatgct gctccatcac cgccaggata taacgcatca tgcgcagtgg gataaattta
    24121 tccggcgaac tctggtgttc tattaaagta taaatgtacc cgtccttccc gtcatttgtc
    24181 ttcatgctga gcaaaacatc gcaggcagac tggcgtaaac tttctgccac aaacgtcgtg
    24241 ggttcaagtt ctattgtatc aagattacag cgggatttct gatacggtgt caatacctgt
    24301 tcaagaaagt ccctcgcggc tgcagggtcc ttcagtgtct tcctgaacgc cgcatcatgt
    24361 atcaggctgg ttttattaac tttttcactc acggaacctc cggataaaaa agtggggcgt
    24421 aacgccccat ggttgatgtt acatggcatg aataattcgt cgttccatgc catacccgag
    24481 ccccataaag ggaatatcat cgtcaaagtc cattggtggt gcaccattac ccgttgatgt
    24541 tgtctggggt gcacgaccag cctgtccggc cgtcttcggc ggctgtcccc agcccccttg
    24601 tgcaggtgca gaagcaccat taccggcagt gccctgctga cgcagtgaac ccagcatctg
    24661 catgacacca ttcactccca caacaacctc agttgaataa cggtccacac cgttctgctg
    24721 gtcaaaccac ttacgggttt tcagctgacc ttcgatatag accttagagc ccttacgaag
    24781 gtactctccg gcgatttctg ccagttttcc ataaataaca atgcggtgcc attcggtcac
    24841 ttccttcatt tcaccgctct gtttatcctt ccaggtatca ctggtcgcca gtgacagatt
    24901 cgccactgca ccaccaccgt taggaaagta cctgacctcc ggatcctgtc ccagatgccc
    24961 aagcaggatg actttgttta cgccacgtga agccatactc ccccctcatt accagtcaca
    25021 acgctgtgtc caggtgccat ttttcagcgc ctgttcagcc tgatcatgtt ttttgaaata
    25081 ctcttcagat tcacgggaca ccggcccctc agaacactga gtgccaatat aatatccacg
    25141 cgtacttttg agaatttcca ggggtaatgt atagccagcc gcagctgcaa gaatgccgtt
    25201 ttgacggttc atatgtgttt ccttaaagtt ttcaaaaacg ataagaaaac agacctccct
    25261 gatggtggtc tgttttcatc atcagggagg ctgaggaaaa tacgatcaga aagagttttc
    25321 tgcgtattga cgtttagttg tgccattttg cggtggcagg gactccgctt tttcagcctt
    25381 atagacctgc tcctggccta ttttaatcca gtcaaccttg atcaggcggg ctttcagact
    25441 gacgcgctgc tcaccggcat gctctccctt gttcagggtg aagatatcgg tggccaggtt
    25501 gcttaacgca aagccaagca aaaccttacg gtcctcatcc actgctttct ggcagcgaga
    25561 gatcagggtg gttgcatctt taccaacaac cgtaacatca aaacgcgtat aggacggatt
    25621 gtccgttggt ccactcagtg cgttaatcac gcagcaaagg aacgagccat tctgatggtt
    25681 aacctgacgt acattactga ggtaccccag accttttacg gtcagattga agtattcagc
    25741 ttttgcagaa gaagtatttg ttgcagacat gatgtgttct ccatggataa aaaaagatat
    25801 gacggagaac accaccgccc ctgacgggtg tgatagttcc cgtcgggggg ccggaatcag
    25861 ctgtatttcc ggctgtctgt gcagtaaaaa aagccttcat cacacgtaaa aagcggtgac
    25921 atctgctctc aaaggtgtgc agatttgccc gtacgtcagg gatgtatccc ttcagtactc
    25981 acagctcctt tcaggctacg gaggtgtaaa actgaccacc cgaaggcgct cctcacaggt
    26041 catatggaac attggtggtc gccggagcga catgggccac ccgaaggcgt tcctcacagg
    26101 ccactatgga acattggtgg tcgccggagc gacaagttag ccatgtcata tttaaagctc
    26161 acacaaagaa agtcaacacc aaaataaaaa gggcaccgta aaaacggtgc ccggggttac
    26221 cagccctgaa gtttatctca tcaagctccg gggatcgctc ccgacgcggt tttcacggag
    26281 tatatgcgcc ttgtgatccc gtcggtgtga aaaacggatc accggacctc agagctgccc
    26341 gctcttttta ctgtggtcag cagggtacgc atcaaagcgt atgcacttta acaaataaaa
    26401 aaaaggaagt aatcatcatc cacgcttttt aatttttctg ccgccgcgtt tactgtgtga
    26461 cgttttctgc tctgaaatca cgcctttaca ctccgggtaa cgctggcaac tccagaactc
    26521 tccgttctta cctttcagct tccgcattct gccctggcaa agtgggcata ccggcgtcac
    26581 tggtgccgta atctgcaact gctgccgtcg ggcgcccgag acgaggtgat tcagccactg
    26641 tttctgcttc tccataaatg acgccagcga cagtttaccc tgtgcaatat catccagggc
    26701 ctgctcccac agtgccgtgg ttcccggact ggtcagcacc tcaggtaatc ctgcaatcaa
    26761 ctctgccccc agttgtgttg cccggatatg cttaccctgt ttctcaatat atttacgggt
    26821 gaacagtgtc tccagtattt cagcacgggt tgcttccgtg cccagtccgg cattctcgcg
    26881 taacactttt ttcagtgccg gatcgctcac cattcccgcc gcatttttca tcgcagcaat
    26941 gagcgtgccc tccgtatagt gacgcggagg cgtggttttc atctctttca gttcagcttc
    27001 aacaagacga cattcatccc ccttagccag aacaggcaga ggcattgtct gctctgtatc
    27061 gcccccctca tcttcatcat gcggcgcatc tgcaataaaa acacgccatc ctttcaccac
    27121 ttccacccgt cctcgcgtgc caaataactg cccggcaaca acaaaagtgg cttcagtgac
    27181 gtccgcttcc tgcacgggta aaaactgagc cagataatgc tgacggatta accggtaaac
    27241 cctgagttcg ttatcactca gggcaccaat attaaacgcc tgacgggtgg ggataatcgc
    27301 atgatgcgca gtgatcttct tatcattcca gactctggaa acaatatcag gactgagttt
    27361 tgccagttca ggggcaatgt ccggatcggt gtttgccaca gccgccagaa cctccggcac
    27421 ttctttatgc attgataccg gcagataacc acagtcagta cggggatacg tcgttgcctt
    27481 gtgtttttcg tacaacgact gggctatatc aagaacggcc tgcgcaccta aaccaaattt
    27541 tttcgagcag acctcctgca gtgtaccgag tgaaaaacaa agcggaggcg gcgtcttctc
    27601 tcttttgctg ctgacctctc tgactacagc aacaccggcc tgacgacact gcgactggac
    27661 gcttgtggca gcctgcacat tcacgcaccg cttttccgca tcacagtact gctctgccgc
    27721 aacccagact gcgcgaaaac gcacgttgtc tttctcaagc cagaggttca gttgccagta
    27781 aggtaccggg ataaaatttt taatttccgc atcccgtctg accaccatcg caagcgtggg
    27841 agtctgcacc ctgccaatcg agagaacacc agacacgccg cactccctgg ctttcatcgt
    27901 gtacaaacgc gtcaggttga tccccgtcag ccagtctgcc cggctgcgcc ccttaccggc
    27961 ctcatataaa cgcgcagttt tctctcctgg aagtattctg cccagcgctt ccctgatact
    28021 gctctcatcc agtgcggata accagaggcg tcgaactact cctgtatagc cacaataatc
    28081 aagcagttct ctggcaataa tttcgccttc ccgatcagca tcggtggcaa tcactacttc
    28141 atctgcctta ccgagaagac gcctgataac attaaactgc ctggcgatct tatccttcac
    28201 caccatctgc cactcttcag gaataaccgg cagaacctca atgctccagg gactgccata
    28261 ctgctcaccg tacgcctccg gtggagcctg ctccagcaaa tgaccaaaag cccatgtcac
    28321 aacaaagtcg ttaccttcaa tcaggccttc accgcgtctg gcagccccaa gcactctggc
    28381 aatgtcactg gcctgtgaag gtttttcgca taaatagaga cgcatagtgt taccagatta
    28441 tttaagagga gcagaaaaag aggaacgttt ctttccacta aggacatcag gatcaggcat
    28501 accgtagcgg ctaatagcct cttttccttt ttgtgatcct gaagaaatat cacttctggt
    28561 tgcgggcaca gttttatagc tctgtgcaag ggcgcagatc tgacgtaccg catgtccacc
    28621 ggcattcagg agctcatcac gacgcgcccg ggcgataaga ccataatgaa acgcctgaaa
    28681 ggctttgagc accaactcat catatcccac aagtaaccag acgctcttat accccagagg
    28741 cgatcggctg tgcacaccaa tgttgacagg actggttgat gcaacaccag aaataataat
    28801 atcccccggc acattccgta aaacctcatt cagttgttca accagtaaat cgatctcctg
    28861 tttggatttg ctcagttttt cctcaaggtt gaacaaaaac atatctgccc acggattgtc
    28921 attctcagaa tcttttgatg cgatttttgc ccttgaaata acacctggca taccaatgat
    28981 tctgcgacga cgctcagact tatcagcgcc aggctcaggc ttgcgacctt cccacaaacg
    29041 aatcgcataa taagtatgaa gattaattga cagagtactt ttcaatacac ccggctcagc
    29101 ctttttctca tcagtcatat gacctcctct cagtaccaga aataaacacg ccttgagtac
    29161 gagatcgtca gaaagaacca gagttacaac aacaagaaaa cagaattgaa cagtgcaaaa
    29221 ttaaaacaag caaacgccat tttcatgtat tcgacaatag aatgcaggtt gtttcttata
    29281 ccgtggtggc agtgccacca tcgctcgttt tttgtaccgt ggtggcagta ccaccatcgc
    29341 tcgttttttg taccgtggtg gcagtaccac cattgctcat tttttatacc gtggtggcag
    29401 tgccaccatc gctcattttt tgtaccgtgg tggcagtgcc accatcgctt attttttgta
    29461 ccgtggtggc agtgccacca ttgctcattt tttataccgc ggtgccagtg gcaccatcga
    29521 ttattttttg taccgtggtg gcagtgccac catcgctcgt tttttgtacc gtggtgccag
    29581 tgccaccatc gctcgttttt tgtaccgtgg tggcagtgcc accatcgctc attttttgta
    29641 ccgtggtggc agtgccatca tcgctcattt tttataccgt ggtggcagtg gcaccactgc
    29701 tcattttttg taccacggta acagtaccac catcgctcaa tttatagatc agcacttaac
    29761 cacgcatagc cagacgggca gcattagcac gctgtatgct ggcacggaat tctgcgacaa
    29821 tatttctcgc tttttctggc gaagccactt ccctgcgacg acgttcacgt tcatcacatc
    29881 catgtccgga ctgagatgac ggacgggcag gctgagactg gatcgttccg cttctgctca
    29941 tatccggcga gttaaagttc ccttcacgcg cacgtttcat cacagccagc atccagccaa
    30001 cgggattctg aagcgtattt ttgctgagca tctgtgacag actgtccagc acaccttccg
    30061 ccagcgtttc cggcaacgcc tgcagttgct tcgtcagcat gccggtgtct tccacactga
    30121 gttgtgattt cagtgcatcc ggcaatacgt acgtattttt tttcatactg tgtgtaatat
    30181 tacgtacgta gtggttcggt ttccgtactg agtgataacc cactgatttt cctggttgtt
    30241 cggaatccgt actgcgttgc gttacctctg ttttttctcc gggttcggtt accgcagtac
    30301 agagttcggt tacctgagca ctgagttcgg ttaccgacgc acatacaggg ggctgtgcgg
    30361 attccgaacc gctcttcgct gcacggccat tcaccgccgg atttacccgc tgacgcgctg
    30421 ccatctgaga cggtgtctgc gctgcgccca tccgtgattc aaacagcgtg atatggctgt
    30481 ggtaatgccg cataccaggt tcatttttga tatcactgag aacctctctg gctgtatacc
    30541 ggatggtctt gttcctgttc ttgcatgcag aatccagcac catatccagg tagcgagggt
    30601 caaacttttc tgcatcacaa cagccaagag gttcatcatg cagggcgtag atatttccac
    30661 ggatacggcc tttttcatca cgaacccgtt tacacagact cagccagccg gtcagccgaa
    30721 gcatggtcag tacccggctt acggtttcac gggatgcctt cccgctaccg ggtgaggcca
    30781 gttgtaactg aagttcatcg taggtgggga atactgcgcc ctcattactc agcgcatgca
    30841 gacgaatcat catccatgcg gttttatcaa gaggagaaag gcgcgtgtcc agcagcagcc
    30901 tacggggcac cgcatcatga acattaccgg taaacagcag cccactgcgt atcctgccct
    30961 cctcacgact ggctttctct gccatgctgg ccttcatttt tctgagcgta tgcgcaataa
    31021 ggctttctgc tggtaacgcc atgattcaat tccccttcaa tcccggaaaa caacaccggg
    31081 gttctgttac tgaaatgctt ataactgcac ccggcaaaga tgcaggctaa acacttcatc
    31141 acccgtaaac cgttaatacc gacggtattg cggttcctct gaaaaaatat tgccttaata
    31201 accagcacgc tgacgcactt cgtttgtcga ctgcttcacc gcaacataaa aaatttatgt
    31261 cacggttcag cagcttcaga actgacataa ccccggcacc tcaggttatc cacggcgatt
    31321 cctttttgat gcatcatcca catgccatgc actggcaagt gcctcagcaa cctgataaaa
    31381 ggaatggcgt acccgggtgg tgcatcttcc ctcgtcagta aagtattcaa cagcggtgag
    31441 ttcccctcca ctttcatggt ccgggtaaaa ctgtgtcacc tcattactgt cgataatcac
    31501 aggacccgaa attgtctgca ttttcagttt catttcactt gttccccttt gatacgaatc
    31561 cccgcggcag tacagacctg ttccagttca gccaccctcc gctcactagc atccagagca
    31621 tcaagcacaa cttttattgc cacggcatgc gccatcggag tgattgcagg aacaggtcca
    31681 gataacaaaa cacgcaactg catcctggcc ttctccagat tcaacattcc actgattttt
    31741 ctcatgaatg attctcctgt tcctgaaggt ctgatatctg tcggcaccaa tgaacagcct
    31801 gacatgcttc ctgtaatacc cagtcaacag cgtctttcca tgctccggtt tcgactggcg
    31861 gattttcacg cttaacctgt tcataaaagc gcacagcctt aaccagtcct tcaggtgcca
    31921 caagcactgg tggcacagcg tagagtggtg tccactttgg cgctttatcc ccgaccgaac
    31981 gctgatacca gtcacccggt ttgtattcat aaaactcacc aaccggctcg gcttccagcg
    32041 acgccagggc aatccgcgcc agttcttccg cttcttctgc aggcagtaca acgttgctac
    32101 ccggtccgta tgtttcgcac cactgcctga ttgtcagcag tcgtcctttg gtaatagtga
    32161 tcatgccgcg tttccttctt tcttattaac aattacaccg tcatatattt cattaaggtg
    32221 ccctctcaac tccatccgcc ttaatgcaga taacatgtaa tcgcattcaa cctgcttatt
    32281 cccggtaaat ggcttatcgt caggattacc ccaacagcaa ttacccctgg gccatccatg
    32341 tactttccga actcttccgt taacaacgtg aagtaatccc cagccaggag gtaaatcctc
    32401 aactgaaata attcccggct cactaataaa gaatcgccag tcgcccatgc caagagaggg
    32461 atttttacgg aaacgctttt ttctatctgc caacaaatca gcacgagaac acttcacctc
    32521 tatcaggcat gatgctgaat ttctgaatcc catagcatct ggctgttctc cagtgctggt
    32581 tacagctata aagcggtcat gaaagcaaac cttgaacccg ttgcgcttaa ggaacttgta
    32641 cgcaatctga cagagttcgc ggtgtgttaa cgccatatca ctctcctttg atgcgaatgc
    32701 cagcgacgcg gattgcagcg atgacttcag aaactttgta tgccattacc gtgtggtaag
    32761 cctcgttaaa atctgttcga tgaagcatac tgctacgttc cgggagcgat atttcccgag
    32821 catccagttc cttaactcgt tcctccagtt cgtagactct gcattgttct ttatcatcaa
    32881 tcagatataa cccaggacat tcgctttcta cccaaccgcc gaaatcatga tcgtaacgct
    32941 cacatgaaaa ctcaccgtca ccgtcctttg ttggaatggt gtaactgtct aatgggccac
    33001 catacgtcgg cacatttccc aatgtcggat gctcaatcca catgaaaaat gcacgtccgg
    33061 ttattgggca aatatctggc cgccattggt tacgaacagc gttggtttcg gataattctt
    33121 cagcgtgttg ttttacttcc tcaagctcaa ctctcagctt cccaaccgta agagcaatat
    33181 cctcattctc ctggtcgcgg gatttaatgt attgctgttt ttcgtccagc tcatccaaca
    33241 gcccggagat aatatccact tcccgatgac ggatgtgatg cttaaacgca gccagagccg
    33301 catcacaatc ccgttcagca tttgggctgt ccgggatgga ctgataccac gccagcgtcg
    33361 actgatagtt ttgtgctgcc ttacgcagtg cctgatagtc aatcttgttc actggttgcc
    33421 tcctgacttt cagccttcag caccatgcgg gagccatcgt ccatttccca cataatttca
    33481 ccaccttctg ccatgaccag atgccagaca agctgagccg cctcattggt tacatcgcgg
    33541 ccgggatcat taccgacacg aagactggtt ccgtcgtcaa gattacgcat ttttgccagc
    33601 acgatagttt ttgaaaacgg agaaaaaccc agctggagtc ttgctgcatt actcatcttt
    33661 ccacctcact tcagtctcat gttgttgtat cgttcatgac tcaacagggc ccaggtcagc
    33721 cctttattcc ggctcagtaa ccgccagtca ggaccaacat caatcttgaa ataaccaaat
    33781 cccctttcct tcagttcatg ataattccgc tctccacgac ggaagcgatt gaccagttca
    33841 accgccttac gctgactggc gggagggacg ccaggacttg tcagcactcc gggaaccagt
    33901 tctgtcatct tcatgacgcg ctccttacct gatccggatt tccggttgta tgccaccctc
    33961 tgacggcatg ccagatggcc gtcagtggaa tgttcatctg ttcagcacaa agcatcatga
    34021 catccagccc atccacactc tccacatcat tgatcccggc tttctgccac aaccgccaga
    34081 gttcagaatt ctcatcgtca ctcagggtga tcccccttcc gggacgaaca tcaataccag
    34141 agatacgacg ccgcgcagcc acatccacgc tggaaagacc aaaataaaac gccatgagtt
    34201 cgatagagcc ccccagcgcc agagcgcgat cgattcgctg cagacgtttc tgttcagtcc
    34261 gcgcctgctg caacatgcgc acaagattgt tgtggttaat atccagccgc agcaccgaca
    34321 cttctgaatt tgacagatag tgaatttctt caatgctcag cccctgcagc atctgcattt
    34381 cttcccggga aagccccagt gcttcacatc gccgcagata accactcttg agatccatca
    34441 ccagttgcat caacagcccg ttggtggcct gtgacaagtt gttactcata cctcacctcc
    34501 gcccgatatc aggctttcag gtctcagatt ccgggaacca acaccatgat gcagtgttgc
    34561 ctttttaccc tcgctgtatc cctgccagcg tgcacgatca ccaccattac agtccttaac
    34621 attccggaag gcagccattc cctcaaattg ctgtctcttc agccacgtct tcattacacg
    34681 cgtctcatct tccgtgagcg ggaatgcatc caccacatca cgcaccccat aaacccagcc
    34741 gtcacgaaac atggctgctc ttgccctgcg ggttgacaat tttaatgtcc tgctctgggt
    34801 tgaaagatac gcctttgttg ccttttgcat ctgccggcag agaacatcaa aggcatacgc
    34861 cgccacaacc gggcgctccc caaagccata aaatatgaca acccgccgcc agtaccttcc
    34921 ggttgtgata cgccagtcca ggtatgtatg gcatccgaat gcctctgcaa caacatgcat
    34981 aaggcgcccc atccataccg gaactttttt cgcttctgac ggggccccct ttgatgacga
    35041 ggtctttatc accgaaatat cagtatccag ttcagtgata ccgtgcttgc gcatcagcgc
    35101 ctgagcacgg gaaagtgcct ggcctgcctc atgggggtta ctgttattcc ggctcagcgc
    35161 cagcagtttt cttatccgtt taatcagttg atcctgacgt gcggcatcag cattcatcgc
    35221 gcccttcctc actctcgtct tccaccatac gctgcagccc ccgcagacga cgcaacacac
    35281 ccagcaggcg aagcaacttt cctgcatgtt catcatcaag caacggcagg tcttcttcag
    35341 tactgccacc tatcagcagt gcatcaaggg aaaccggatt gacccgcaga acctcctcac
    35401 cggtcagtct gacaagaaat gccatgaccg gtgagcaatt tgcctctgcc gcaccaaatc
    35461 ctgcagacag ctcactttcc cggtccatcg ctatctcatc gacacagcct gtatgtgacg
    35521 ccagctccca tgcgaggtga aacgcctcat tctgcagaga ttcgatatca tcctgttcag
    35581 caccagtgaa tgcagacgcc tggcgaaatt ctcccccctc ctgcatggcg ttatcttccg
    35641 gcattatcgg aatatcagtt gacgcaccgg tcgcatcctc tccgcaatca ccgctgtatg
    35701 agtcttcgcc accatacata tccggttgaa cttcctttct gcgtcgttcg catttatctg
    35761 cgaccgggcg gtcctctttc tcagcccctg attgcgcagt atccatgacg ctctcacggt
    35821 ctgcgggagt ggcggcaacg acctgagcaa cttccgtcgc cccgccagac gccacctgcg
    35881 cccccggttc tggggggctg tccgccggca tgagtaccgg ttcgggttca ccaaaatggt
    35941 gacgtcgatt acgctcccgg ggatccagct ccagcaacca gcgatcgtaa tccagtaccg
    36001 gatgtggcag agccttcagc aggtcaccga tcagctcatc cctgaacatt tccatggacc
    36061 agagttccgg cgagttgaat ttgccgcaac attcaccaaa cacgcgatca aaatccacat
    36121 cgttttccgc cgcgactgca aactgccccc agatgcgttc cgcatcctga cggagagaca
    36181 gtaaggctct gacctgcgga ctccccatac ctgactccag aagatcaggg atccacggat
    36241 acagatattt cagcgcatgc tccattctgc tgacggatga atgatttaca ggaagccctt
    36301 catctgtcag tagtgatgcc agttctctga tcgtcacagt ccgctgcagg gactcttcat
    36361 aaatcatccg tgctttatgg ataccctgag ccttttcaat aaacgtcagt tcacccctga
    36421 cttcattctc agccagatga ccaatgacac actgaagccg ccccggccac ggtttgaaaa
    36481 gcacatgaat acggtagaaa cgctgctcgc cggtctcctc ccacagttct ctcaggatct
    36541 gataacgagt gttaccacca tcgctgaaga tgtagatatc cggctcactc tccggtatcc
    36601 gggtgacctt tggcaccgta tccagtccgc gggagaaaat ggaactttta atatcgtcgt
    36661 accggggatt gcgcgtggtt ctcgggttat caggattagg gcgcaactga tccagcgtca
    36721 gaaccatggg cgtttccccc agggcaacca cgttaccggt cttactggcc tggcgccccg
    36781 gttgcatgat ggcggccccc agatcaaggg aatgatttcg cttaattgac atacgcattg
    36841 cctccggaac ggtcaaaatt ccagccacgt tcaaacggtg aaaacagggt gtaacgacca
    36901 tcgaagctca cccgtatcgt accggtcggt ccctgtcgct gtttgctcac aatgatttcc
    36961 gccacgccct tatccagagt gccggggttg tagacctcgt cacggtaaat aaacataatg
    37021 aggtcagcat cctgctccag cgccccggaa tcacgcaggt caccgttgtt tggtcgttta
    37081 tctgcccgct gttccacctg tcggttaagc tgcgacagtg cgagcacagg gcatccaagt
    37141 tctttcccca gcgacttgag agcgcgggat atttccgcaa tctcctgagt gcggttttcc
    37201 tggtccggtg aacgaatcaa ctggagataa tcgaccatta tcagggaagg tctgccatac
    37261 agacgcacat aacgacgggc actggcgcgc agtgatgacg gagtctgaga tgacgtgtca
    37321 tcaatgatca ggcggttacc ttcaccggca aatgtcccct cctgcatgaa ctcacccatt
    37381 gttgccgtaa gtctcgccca ctcctcatcg tccatcatgc tgctgcgaag acgagtcagc
    37441 tccacccggg acagcatcga cagggtacgc agaatcagct gctccctgga catttctatg
    37501 ctgaacagga ataccgtttc atcggtccgc tcacgcaggg cattaagaca cagagtgagt
    37561 ccaagggccg ttttccccat cgacggacgt gctgccagaa ggataagatc cccgggctgc
    37621 aggccacagg tcatctcatc cagttcatc
//
LOCUS       DAZGVQ010000030        36993 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000030 DAZGVQ010000000
VERSION     DAZGVQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.030"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(90..173)
                     /gene="yohP"
                     /locus_tag="ACHLAG_001487"
     CDS             complement(90..173)
                     /gene="yohP"
                     /locus_tag="ACHLAG_001487"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFP0654745.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            397..1833
                     /gene="mdtQ"
                     /locus_tag="ACHLAG_001488"
     CDS             397..1833
                     /gene="mdtQ"
                     /locus_tag="ACHLAG_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFP0654746.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            1886..2647
                     /gene="yohF"
                     /locus_tag="ACHLAG_001489"
     CDS             1886..2647
                     /gene="yohF"
                     /locus_tag="ACHLAG_001489"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0654747.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSYVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(2777..3355)
                     /gene="yohD"
                     /locus_tag="ACHLAG_001490"
     CDS             complement(2777..3355)
                     /gene="yohD"
                     /locus_tag="ACHLAG_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP0654748.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHTLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            3525..4112
                     /gene="yohC"
                     /locus_tag="ACHLAG_001491"
     CDS             3525..4112
                     /gene="yohC"
                     /locus_tag="ACHLAG_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFP0654749.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            4286..5218
                     /gene="pbpG"
                     /locus_tag="ACHLAG_001492"
     CDS             4286..5218
                     /gene="pbpG"
                     /locus_tag="ACHLAG_001492"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFP0654750.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(5256..6971)
                     /gene="dld"
                     /locus_tag="ACHLAG_001493"
     CDS             complement(5256..6971)
                     /gene="dld"
                     /locus_tag="ACHLAG_001493"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP0654751.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFHPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            7167..9464
                     /gene="bglX"
                     /locus_tag="ACHLAG_001494"
     CDS             7167..9464
                     /gene="bglX"
                     /locus_tag="ACHLAG_001494"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFP0654752.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            9675..10592
                     /gene="osmF"
                     /locus_tag="ACHLAG_001495"
     CDS             9675..10592
                     /gene="osmF"
                     /locus_tag="ACHLAG_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFP0654753.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVREAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            10599..11756
                     /gene="yehY"
                     /locus_tag="ACHLAG_001496"
     CDS             10599..11756
                     /gene="yehY"
                     /locus_tag="ACHLAG_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFP0654754.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            11749..12675
                     /gene="yehX"
                     /locus_tag="ACHLAG_001497"
     CDS             11749..12675
                     /gene="yehX"
                     /locus_tag="ACHLAG_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFP0654755.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALVEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            12680..13411
                     /gene="yehW"
                     /locus_tag="ACHLAG_001498"
     CDS             12680..13411
                     /gene="yehW"
                     /locus_tag="ACHLAG_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFP0654756.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(13392..13499)
                     /gene="yohO"
                     /locus_tag="ACHLAG_001499"
     CDS             complement(13392..13499)
                     /gene="yohO"
                     /locus_tag="ACHLAG_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFP0654757.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(13559..14290)
                     /gene="mlrA"
                     /locus_tag="ACHLAG_001500"
     CDS             complement(13559..14290)
                     /gene="mlrA"
                     /locus_tag="ACHLAG_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFP0654758.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            14512..16197
                     /gene="btsS"
                     /locus_tag="ACHLAG_001501"
     CDS             14512..16197
                     /gene="btsS"
                     /locus_tag="ACHLAG_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFP0654759.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            16194..16913
                     /gene="btsR"
                     /locus_tag="ACHLAG_001502"
                     /gene_synonym="yehT"
     CDS             16194..16913
                     /gene="btsR"
                     /locus_tag="ACHLAG_001502"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFP0654760.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLEPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            16960..17430
                     /gene="yehS"
                     /locus_tag="ACHLAG_001503"
     CDS             16960..17430
                     /gene="yehS"
                     /locus_tag="ACHLAG_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFP0654761.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKG"
     gene            complement(17470..17931)
                     /gene="yehR"
                     /locus_tag="ACHLAG_001504"
     CDS             complement(17470..17931)
                     /gene="yehR"
                     /locus_tag="ACHLAG_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFP0654762.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(18056..20056)
                     /gene="yehQ"
                     /locus_tag="ACHLAG_001505"
     CDS             complement(18056..20056)
                     /gene="yehQ"
                     /locus_tag="ACHLAG_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFP0654763.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRALQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(20053..21189)
                     /gene="yehP"
                     /locus_tag="ACHLAG_001506"
     CDS             complement(20053..21189)
                     /gene="yehP"
                     /locus_tag="ACHLAG_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP0654764.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(21182..23461)
                     /gene="yehM"
                     /locus_tag="ACHLAG_001507"
                     /pseudo
     CDS             complement(21182..23461)
                     /gene="yehM"
                     /locus_tag="ACHLAG_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            complement(23472..24560)
                     /gene="yehL"
                     /locus_tag="ACHLAG_001508"
     CDS             complement(23472..24560)
                     /gene="yehL"
                     /locus_tag="ACHLAG_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFP0654765.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            complement(25133..25453)
                     /gene="yehK"
                     /locus_tag="ACHLAG_001509"
     CDS             complement(25133..25453)
                     /gene="yehK"
                     /locus_tag="ACHLAG_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFP0654766.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     LIEY"
     gene            complement(25514..29146)
                     /gene="yehI"
                     /locus_tag="ACHLAG_001510"
                     /pseudo
     CDS             complement(25514..29146)
                     /gene="yehI"
                     /locus_tag="ACHLAG_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
     gene            complement(29156..32949)
                     /locus_tag="ACHLAG_001511"
                     /pseudo
     CDS             complement(29156..32949)
                     /locus_tag="ACHLAG_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            complement(33090..35123)
                     /gene="metG"
                     /locus_tag="ACHLAG_001512"
     CDS             complement(33090..35123)
                     /gene="metG"
                     /locus_tag="ACHLAG_001512"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP0654767.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            35255..36364
                     /gene="apbC"
                     /locus_tag="ACHLAG_001513"
     CDS             35255..36364
                     /gene="apbC"
                     /locus_tag="ACHLAG_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFP0654768.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            36627..36908
                     /gene="yehE"
                     /locus_tag="ACHLAG_001514"
     CDS             36627..36908
                     /gene="yehE"
                     /locus_tag="ACHLAG_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFP0654769.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         8716 a         9315 c         9850 g         9112 t
ORIGIN      
        1 tatatgaagc aatacacatg acaggagcgc aacaggatga gagactgaaa tattctcgtt
       61 attttcggac ctgatgacat taattaattt taaaatatca tcttaaatac gccagtcacc
      121 accagtagcc caatcaggaa aataatcaat acagcccaga gtataatttt cattggcgtt
      181 cctttatttg tgagtgctga ataagtatag gaaaaaacga agccctttgc cgttatccat
      241 aaacgattaa gataaatcgg aagatatcat tcacttagtg tatatttttc tggatatgtc
      301 gttttattgc tactgatgta ttgccacgcg aaactaaaaa atatatcgca acacacggta
      361 atctccgcgc accttttctt acctgattat tcccccatga atcgtgattc cttttatccc
      421 gccatcgcct gttttccgct gttactgatg ctggccgggt gtgcgcctat gcatgaaacc
      481 cgccaggcgt taagccagca aacgcccgct gcacaagttg acaccgcatt acccacggcg
      541 ctgaaaaatg gttggccaga cagccaatgg tggctggagt atcacgataa tcaactcact
      601 tccttaatta acaatgcact acagaacgca ccggatatgc aggtggctga acagcgcatt
      661 cagctagctg aagcccaggc gaaggcaatt gctactcagg atggtccgca gatcgacttt
      721 tctgcggata tggagcggca aaaaatgtcg gcagaaggct taatggggcc gtttgctctg
      781 aacgatccgg ccgcaggtac gaccggtccg tggtacacca acggaacttt tggcttaacg
      841 gcgggctggc atctcgatat ctggggaaag aatcgggcgg aggttactgc ccgcctgggt
      901 acggttaaag cacgggcggc ggaacgcgag caaacccgcc aattgctggc tggcagcgta
      961 gcccgcctgt actgggagtg gcaaacccag gcggcgttaa acacggtctt gcagcaaata
     1021 gaaaaagagc agaacaccat tatcgcgacc gatcgccagc tatatcagaa cgggattact
     1081 tcttcagttg aaggtgtgga aaccgatatt aatgccagca aaacccggca gcagctcaac
     1141 gatgtcgcgg ggaaaatgaa aattattgag gcacggttaa gcgcacttac aaataaccag
     1201 acaaagtcat tgaagcttaa accggtcgcg ttgccgaaag tggcaagcca gcttcctgat
     1261 gaactggggt actccttact ggcccggcgg gcagatttgc aggcggcgca ctggtacgtt
     1321 gagtcatcgc taagcaccat tgatgcggca aaagcggcat tttatcctga catcaacctg
     1381 atggccttcc tgcaacagga tgcgttgcac ttaagcgatc tgttccgtca ttccgcgcag
     1441 caaatgggcg ttacggcagg cctgacgcta cccattttcg atagtggtcg tcttaacgcc
     1501 aatctcgata tcgcaaaagc cgaaagcaac ttgtctatcg ccagctacaa caaagcggtg
     1561 gttgaagcgg tgaatgacgt ggcgcgggca gccagtcagg ttcagacact tgcggagaaa
     1621 aaccagcatc aggcgcaaat tgagcgcgat gccttgcgtg tggtaggtct tgcgcaggcg
     1681 cgctttaacg cgggcatcat tgctggttcc cgcgtcagcg aagccagaat ccccgcgctg
     1741 cgtgagcggg ccaatggcct gttattgcaa gggcagtggc tggatgcttc cattcaactc
     1801 actggtgcgt tgggcggggg ttacaaacgc tgatgatatg cgccttctat acttaacgtt
     1861 tattcagcgt taagtggaga actcgatggc acaggttgcg attattaccg cctccgattc
     1921 ggggatcggc aaagagtgcg cgttattact ggcgcagcag gggtttgata ttggcattac
     1981 ctggcactca gatgaagaag gggcaaaaga taccgcgcgt gaggtagtta gccacggcgt
     2041 acgtgcggag attgtgcagc tggatctcgg taaactgcca gaaggtgcac aggcgctgga
     2101 gaaactcatt caacggctgg ggcgtattga tgtgctggtg aataatgcgg gcgcaatgac
     2161 caaagcgccg tttcttgata tggctttcga tgagtggcgc aagattttta ccgttgatgt
     2221 cgatggtgca tttttatgct cacaaattgc ggcccgccag atggtgaaac aagggcaggg
     2281 cggtcgcatc atcaacatta cgtcggtaca tgaacatacg ccgctgccgg atgccagcgc
     2341 ttacacagcc gctaaacatg cgctcggcgg gttaaccaaa acgatggcgc tggagctggt
     2401 caggcataag attttggtga acgcagtcgc gcctggggcg atcgccacgc caatgaatgg
     2461 catggatgac agctacgtga agcccgacgc ggagccttcg attcctctgc ggcgttttgg
     2521 cgcaacgcat gagattgcca gcctggtagc gtggctgtgc tcagagggcg caaattacac
     2581 caccgggcag tcgttgatag tggatggcgg ctttatgttg gcgaatccgc agttcaaccc
     2641 agaatagcgc agtgaggtgc gtggttattt caatgggtaa aattttggta ggcatgataa
     2701 gacgcacaag cgtcgcatca ggcatggtgc cccggatgtc ggatgcggcg cgaacgcctt
     2761 atccagggca atggttttac gcctgattat ccggcttttt cttcccgcgt cgtttcagcc
     2821 accagcgcac gcctaccacc agaaccacga ccagaatcaa ccagacccag tgcttcaaat
     2881 gctggtcgag agtgtgcaac cacggcgcaa tcacctgacc accagcgtaa ccaatagtgg
     2941 taaaaatcaa cgcccaggca aatgcgccga gaatattcag cggcagaaag attttcggcg
     3001 gcagctggct ggcaccaatc agcgtcgggc caatcacccg aaagccatac ataaagcgcg
     3061 taccaatgac aaacagatac ggatggcgtt ggataagttt ctgcgcccgc tcaattttat
     3121 cctgatgttt cgagaaacgg cgtaacagct tgccgccaaa ccgccgcccg cacagataga
     3181 gcacctggtc gccaatcatg ccgccaagcg ccacagaaag taccaccagc gggaacttta
     3241 atagcccctg atgcgccgca acgcctccca gcaaagtcac ggtttcacct tccgccaggc
     3301 taccgatcac cagcgcggca taaccatatt gtgagataag tgtattgaga tccataggcc
     3361 agtctgtctc cctttggttg ccagtaagca tacaccctgt gaatcattca cgctgaaaaa
     3421 ggacgttcat cagacagtga aaattacatg ggcgaacaaa aaataatcac aagctgactt
     3481 atacttgaac tgtcaggttc atgaactcac aacaaggggg cggtatgagc catgtctggg
     3541 gactgttttc ccatcccgat cgtgaaatgc aggtgattaa tcgcgaaaac gaaacgattt
     3601 ctcaccatta cacccaccac gttttgctga tggcggcgat tccggtgatt tgcgccttca
     3661 ttggcactac acagattggc tggaattttg gcgatggcac tatcctgaag ttatcctggt
     3721 ttaccggact ggcgctggct gtcttgtttt acggcgttat gctggctggg gtcgcggtga
     3781 tggggcgggt catctggtgg atggcgcgta attatccgca gcgtccgtca cttgcgcact
     3841 gcatggtctt cgcgggctac gttgcaactc cgctgttttt aagcggtctg gtggcgcttt
     3901 atccactggt ctggttgtgt gcgctggtcg gcacggtggc gctgttttac accggctacc
     3961 tgctttacct tggtattccg agcttcctga atatcaacaa agaggaaggc cttagcttct
     4021 ctagttcgac gctggcgatt ggcgtgctgg tactggaagt gctgctcgcc ctaaccgtta
     4081 tactatgggg ctacggttat cgtttgttct agaaagttct ttgacgttgc attgctggcg
     4141 taaatgtcag caatgcagca tttcctcacg attctccttt gacgatcgca ctttttgctc
     4201 gttatgatgg cgtcgctagc ctcagtaaat ccttactccg gcggtgcgca acccgtgcgc
     4261 gtgaaccact atctgaatgc tcatcatgcc gaaatttcga gtttctttat ttagcctggc
     4321 cctgatgctg gctgtgcctt ttgcaccgca ggccgttgcg aaaacggcag ccgctaccac
     4381 cgcttcacaa ccggaaattg cctccggtag cgcgatgatt gttgatctga ataccaacaa
     4441 agtgatctat tcgaaccacc cggatctggt gcgtccgatt gcgtctatca gcaaattaat
     4501 gaccgcgatg gttgtgctgg atgcacgact gccgctggat gaaaaactaa aagtggatat
     4561 cagccagacg ccggagatga aaggggtcta ttcgcgcgta cgactgaata gcgaaatcag
     4621 ccgtaaagat atgctgttgc tggcgctgat gtcttcagaa aaccgcgcgg cggcaagcct
     4681 tgcgcaccat tatcccggtg gttacaaagc ctttattaag gcaatgaatg cgaaagcgaa
     4741 atcgctcgga atgaacaaca cgcgctttgt tgaacctacc ggattgtcgg tgcataacgt
     4801 ttcaactgcc cgtgacttaa ccaaactgct cattgccagc aaacagtatc cgttgatcgg
     4861 gcagttaagt actacccggg aagatatggc aaccttctct aacccgacgt atacgctgcc
     4921 gttccgcaat actaatcatc tggtgtatcg cgataactgg aatattcagt taaccaaaac
     4981 cggctttacc aatgcggcgg gccattgtct ggtgatgcgt acggttatca ataataaacc
     5041 ggtggcgctg gtagtgatgg acgcgtttgg caaatatacc cattttgccg atgccagccg
     5101 cctgcgtacc tggattgaaa ccggtaaagt gatgcctgtg ccggcagcag cgttgagcta
     5161 taaaaaacaa aaagccgccc aaatggcggc ggcggggcag acggcacaga acgattaagt
     5221 gaattcggat ggcgatactc tgccatccgt aatttttact ccacttcctg ccagtttttc
     5281 cgtttactgg ttttaccgat tcccggattc atgctgttgg tcggatcgtt ctcgcgatag
     5341 aacttctgca acgtctccgg tgctttatac aaatgaccga cgttatgctc ggcagggtat
     5401 tgcgcgccgc gctgctgtag cagttccagc atctgttctt ttaacgcatg cacatccacg
     5461 ccttttttca ctatgtaatc ctgatggaag acatagcaca taaaatggcc gtaatagagc
     5521 ttgtgcacca gctggctgtc gatctccggc ggtaaatgct cataccactc ggtgtcgtta
     5581 cgccgcagag cgatatccaa cgccagaatg tcttcgactt catcggaatg caccgcctga
     5641 taacgaattg ctgcgcccgc agcggcgaaa cggtgtaaaa acgctttgct gccttcctcc
     5701 ggcgtacaga caaagaaatc gccttcggcc tgtttgaaat aatccaccaa ccacgatttg
     5761 gcttcgccca cgccatcgcc cgccattttt aacagcagat gatgctcgta tttatcgcgc
     5821 cagtttttca tgcgcggcgg taaatggctg gggaacaggt gaccgaattt ttgcatcgca
     5881 cggtcagtaa aatgcggatg gaagaatttc actttctcca gcatcgcatc ggtgcgtccc
     5941 ttgagattaa agaagaacgg catcttgtcg gtgcctaact tatcaatcat caggaaggtg
     6001 tctttgccgt atttttccgc aatatcgtag atatcccggt gcatatattc cccggcaacc
     6061 ggcagatttt cgaagttagc cagaatatga cggcggattt cggtcagcac ttccggctgg
     6121 ttggtgccga tataaaacac ctgctgattt ttttccgctt cgaaggtatc tagacgtacc
     6181 gcaaagacgg ccagcttccc ggcgcaacca gaagattcaa ataaccgatc cggatcggcg
     6241 ttataacgtg cgggcgtgtc ggcttcaata tcacgaacgc ggtggacata gtcataatcg
     6301 tgggcgtgac gaccatcgtg acgcacatcg tcatctttga tgcgatcatc atccagcttg
     6361 ctaaggatct gctctggcgt ttcgcccaga tcaatcccca gatggttcac cagcgtcagt
     6421 ttgccgtctt catttatacg cgcgaataac gacatttcgg tatacgccgg gccacgttgc
     6481 accagcgagc cgccggagtt gttacaaata ccgccgataa ccgatgcgcc tatacacgac
     6541 gatccaatca ccgagtgcgg ttcgcgtccc agcggtttga gggctttttc cagcgaatag
     6601 agcgtggtgc ccggataggc tagcacctgt tcgcccttgc caagaacgtg cagcttgtcg
     6661 agacgcaggg tgctgatgat aacgacatct cgatcatagt cgttaccgtt tggcgtcgat
     6721 ccttcggtca ggcctgtatt ggcagcctgc atcagaataa ttttgtcggc ggtgacgcag
     6781 gctttcagca cccgccacaa ttccagtagt gagccaggga aaacgacagc cagcgcgtcg
     6841 ccctgaccag aacggaagcc cttgcgatag cgggccgttt ttgcgggatc ggtgagcagg
     6901 tgtgaatgac cgaccagacg ggcaagttca ttcaaaaagg ctttattatc agttgttgtc
     6961 atggaagaca ttttccactc cttgtggtgg cgaaaaaata tcacaaacta gaatagcgcc
     7021 ttgtatgaca attcagcgag tgaatatttt gaaaattcag acaataaact gagcattctt
     7081 tttctctctc catcatgctt atggcaaact acggtttttg caccattttc gctgcctcgt
     7141 cgggcaacag aggaagaaaa atccatatga aatggctatg ttcagtagga atcgcggtga
     7201 gtctggccct gcagccagca ctggcggatg atttattcgg caaccatcca ttaacgcccg
     7261 aagcgcggga tgcgttcgtc accgaactgc ttaagaaaat gacagttgat gagaaaattg
     7321 gtcagctgcg cttaatcagc gtcggcccgg ataacccgaa agaggcgatc cgcgagatga
     7381 tcaaagacgg tcaggttggg gcgattttca acaccgtaac ccgtcaggat atccgcgcca
     7441 tgcaggatca ggtgatggaa ttaagccgcc tgaaaattcc tcttttcttt gcttacgacg
     7501 tgctgcacgg tcagcgcacg gtgttcccga ttagcctcgg tctggcctcg tcttttaacc
     7561 tcgatgcagt gaaaacggtc ggacgtgtct ctgcttatga agcggcagat gatggcctga
     7621 atatgacctg ggcaccgatg gtcgatgtct cgcgcgatcc gcgctgggga cgtgcttccg
     7681 aaggttttgg cgaagatacg tatctcacct caacaatggg taaaaccatg gtggaagcga
     7741 tgcagggtaa aagcccggca gatcgctact cggtgatgac cagcgtcaaa cactttgccg
     7801 catacggcgc ggtagaaggc ggtaaagagt acaacaccgt cgatatgagt ccgcagcgcc
     7861 tgtttaatga ttatatgccg ccgtacaaag cggggctgga cgcaggcagc ggcgcggtga
     7921 tggtggcgct gaactcgctg aacggcacgc cagccacctc cgattcctgg ctgctgaaag
     7981 atgttctgcg cgaccagtgg ggctttaaag gcatcaccgt ttccgatcac ggtgcaatca
     8041 aagagctgat taaacatggc acggcggcag acccggaaga tgcggtgcgc gtggcgctga
     8101 aatccggaat caacatgagt atgagcgacg agtattactc gaagtatctg cctgggttga
     8161 tcaaatccgg caaagtgacg atggaagagc tggacgatgc tgcccgccat gtactgaacg
     8221 ttaaatatga tatgggattg tttaacgacc catacagcca tttggggccg aaagagtctg
     8281 acccggtgga taccaatgcc gaaagccgcc tgcaccgtaa agaagcgcgt gaagtggcgc
     8341 gcgaaagcct ggtgttgctg aaaaaccgtc tcgaaacgtt accgctgaaa aaatcggcca
     8401 ccattgcggt ggttgggcca ctggcggaca gtaaacgtga cgtgatgggc agctggtccg
     8461 cagccggtgt tgccgatcaa tccgtgaccg tactgaccgg gattaaaaat tccgtcggtg
     8521 aaaacggtaa agtgctgtat gccaaagggg cgaacgttac cagtgacaaa ggcattatcg
     8581 atttcctgaa tcagtatgaa gaagcggtca aagtcgatcc gcgttcgccg caagagatga
     8641 ttgatgaagc ggtgcagacg gcgaaacaat ctgatgtggt ggtggctgta gtcggtgaag
     8701 cacaggggat ggcgcacgaa gcctccagcc ggaccgatat cactattccg caaagccaac
     8761 gtgacttgat tgcggcgctg aaagccaccg gtaaaccgct ggtgctggtg ctgatgaacg
     8821 ggcgtccgct ggcgctggtg aaagaagatc agcaggctga tgcgattctg gaaacctggt
     8881 ttgcggggac tgaaggcggt aatgcaattg ccgatgtatt gtttggcgat tacaacccgt
     8941 ccggcaagct gccaatgtcc ttcccgcgtt ctgtcgggca gatcccggtg tactacagcc
     9001 atctgaatac cggtcgcccg tataatgccg acaagccgaa caaatacact tcgcgttatt
     9061 ttgatgaagc taacggggcg ttgtatccgt tcggctatgg gctgagctac accactttca
     9121 ccgtctctga tgtgaaactt tctgcgccga ccatgaagcg tgacggcaaa gtgactgcca
     9181 gcgtgcaggt gacgaacacc ggtaagcgcg agggggccac ggtagtgcag atgtacttgc
     9241 aggatgtgac ggcttccatg agtcgccctg tgaaacagct gaaaggcttt gagaaaatca
     9301 ccctgaaacc gggcgaaact cagactgtca gcttcccgat cgatattgag gcgctgaagt
     9361 tctggaatca acagatgaaa tatgacgccg agcctggcaa gttcaatgta tttatcggca
     9421 ctgattccgc acgcgttaag aaaggcgagt ttgagttgct gtaatgcttt agtaagggcg
     9481 cgacgtttgc gccctttgta ggccggataa ggcgctcgcg ccgcatccgg caggaaacgc
     9541 cggaccgaat atgcctctta agtatttatc tgattctgca actgaatcct tccgctcaag
     9601 ctaaccccgc cattatcaac tatgcttttc tcttaattcg ctgaaaacaa cagtaaaaga
     9661 atgaggaaag catcatgcca ctcttaaagc tctgggcagg ttcactggtt atgttggcag
     9721 ccgtgagcct gccgctacag gcggcttccc ccgttaaagt cggttcaaaa atcgataccg
     9781 aaggtgcgct actcggcaat atcattttgc aggtactgga aagccacggc gtaccgacgg
     9841 tgaataaagt gcaactcgga acgactcctg tggtgcgcga ggcgattact tccggtgaac
     9901 tggatatcta tccggaatat accggcaatg gcgctttctt ctttaaagat gaaaacgatg
     9961 cagcgtggaa aaacgcgcag caaggttacg agaaagtcaa aaagctcgat tcggagcaca
    10021 acaagttaat ctggctgacg cccgcgcctg caaataacac ctggactatc gccgtgcgtc
    10081 aggatgtggc agagaaaaat aaactaactt cgcttgccga cctgagtcgt tatctgcaag
    10141 agggcggcac cttcaaactg gcagcctcgg cagagtttat cgaacgcgcc gatgcgttac
    10201 ccgcgtttga aaaagcctat ggctttaagc tcggtcagga tcagttgctg tcactggctg
    10261 gtggcgacac ggcggtgacg atcaaagccg ctgcccagca aacctctggc gttaatgctg
    10321 caatggctta cggcactgac ggcccggtag cggcgctggg gctgcaaacc ttaagcgatc
    10381 cgcaaggcgt gcaacctatc tatgcgcctg caccagtggt gcgtgaatcg gtgttgaagg
    10441 agtatccgca aatggcacag tggctacagc cagtcttcgc cagcctcgat gcaaaaacat
    10501 tgcagcaact gaatgccagc attgcagtgg aaggactgga tgccaaaaaa gtggctgccg
    10561 actacctgaa acaaaaaggg tgggcgaagt aatttcccgt gacttatcta cgtattaatc
    10621 ctgttctggc gctgctgctg ttgctgacgg caatcgcagc agcgctgccg tttatcagct
    10681 acgcgcctaa tcgtttagtc tcgggtgaag ggcgtcatct ctggcaactg tggccgcaaa
    10741 cgatctggat gctggtgggc gttggttgcg cctggctgac agcctgtttt gtccccgcta
    10801 aaaaaggcag catttgtgca ctcattctgg cgcaattcgt cttcgtattg ttggtgtggg
    10861 gagctggaaa ggcggcgacc caactggcgc aaaatggcag tgcgctggcg cgtaccagcc
    10921 tcggcagtgg tttctggctg gctgcggcgc tggcattgct ggcctgtagc gatgccatcc
    10981 gccgaatctc cacgcatccg ctgtggcgct ggttgttgca tatgcagatt gccattattc
    11041 cgctgtggtt gctgtactcc ggcacgctta acgatctctc actaatgaaa gaatacgcca
    11101 accgtcagga tgtgtttgac gacgcgctgg cacaacatct gacgttgctg tttggtgcgg
    11161 tgctgcctgc gttagtgatt ggtgtgccgt tgggcatctg gtgctacttt tccactgcgc
    11221 ggcagggggc aattttttct ctgctcaatg tcattcagac cgtgccttcg gtggcgctct
    11281 ttggcctgtt gattgcgccg cttgccgcgc tggttacggc ctttccgtgg ctggggaagc
    11341 tcggcatagc aggaaccgga atgacacccg cactgattgc gctggtgctc tatgccttgc
    11401 tgccgctggt gcgcggcgtg gtagtcggtt tgaaccagat cccgcgcgat gtgctggaga
    11461 gcgccagagc gatgggcatg agcggggcgc agcgattcct gcatgttcag ttaccactgg
    11521 cgttaccggt atttctgcgc agcctgcggg tggtgatggt gcaaactgta ggtatggcgg
    11581 tgattgcggc gttaatcggc gcaggcggtt ttggtgcgct ggttttccag gggctgctaa
    11641 gcagcgccat tgatttagtg ttgctggggg tgatcccggt aattgttctg gcggtgctga
    11701 tcgatgccct gttcgatttg cttatcgcac tgctgaaggt gaaacgtaat gattgaattt
    11761 agccatgtca gcaaactgtt tggcgcacaa aaagccgtta acgatcttaa tctcaatttt
    11821 caggaaggga gtttttcggt gctgattggc acatctggct ccggcaaatc caccaccttg
    11881 aaaatgatta accgcctggt ggaacatgac agcggagaga tccgctttgc cggagaagaa
    11941 attcgctcgc tgccagtact ggagttgcgc cgccggatgg gctatgccat tcaatctatt
    12001 ggcctgttcc cccactggag cgtggcgcaa aacatcgcta ccgtgccgca attacaaaaa
    12061 tggtcgcggg cacggattga cgatcgtatc gacgaattaa tgacgctact ggggctggag
    12121 tcaaatttgc gtgagcgtta tccgcatcag ctttccggtg gtcagcagca acgtgtggga
    12181 gtggcgcgcg cactggctgc cgatccgcaa gtcttactga tggatgaacc ttttggcgca
    12241 ctggacccgg taacgcgcgg cgcgttgcaa caagagatga cgcgcattca ccgtttgctg
    12301 gggcgcacta ttgtgctggt cactcatgat attgatgagg cgctacggct ggctgaacat
    12361 ctggtattga tggatcacgg tgaagtggtg cagcagggca atccgctgac gatgctgact
    12421 cgtccggcga atgattttgt ccgccagttt tttggacgta gcgaactggg tgtgcgtctg
    12481 ctttcgttac gtagtgtggc ggattacgtg cgtcgcgaag aacgggcaga aggtgaggca
    12541 ctggtagaag agatgacgct acgcgatgcg ctctccctgt ttgtcgcgcg gggttgcgag
    12601 gtgctgccgg tggtgaacac gcagggccag ccttgcggta cgctgcattt tcaggatctg
    12661 ctggtggagg cgtaagcgta tgaagatgtt gcgcgatccg ctgttctggc tcattgcttt
    12721 gtttgtggca ctgattttct ggctgcctta cagccagccg ctgtttgctg ccttgttccc
    12781 acaactgcca cgacccgttt atcagcaaga aagttttgca gctctggcac tggctcattt
    12841 ctggctggtg ggaatttcga gtttgtttgc ggtgatcatt ggcactggtg ccggaattgc
    12901 tgtcactcgc ccgtggggcg cggaatttcg cccactggtg gaaactattg ccgccgttgg
    12961 acagactttt ccgcctgtcg cagtgctggc gattgccgtt ccggtgatcg gctttggtct
    13021 gcaaccagcg attatcgcct tgatccttta cggtgtgctg cccgtcctgc aggcgacact
    13081 tgccgggctg ggagcgatcg atgccagcgt gacagaagtt gcgaaaggta tgggaatgag
    13141 tcgtggtcag cgactgcgta aggtcgagct accgctggcg gctccggtga ttctggcggg
    13201 cgtgcgaact tcggtgatta tcaacattgg tacggcgacg atcgcctcaa cggtaggagc
    13261 cagcacgctg ggtacgccca tcatcatcgg gcttagcgga tttaataccg cgtatgtgat
    13321 ccagggggcg ttactggtgg cactggcggc gatcatcgca gaccgcctgt ttgaaaggct
    13381 ggtgcaggcg cttagccagc acgcaaaata aaggtataac ctgcgagcat gacgccacca
    13441 attccgccga acgccataaa caggaacagg gcgatgaccc caattttagc tatgcgcata
    13501 atgcactcct tatgttaacg aaaggattgt acagtaaagc gcatttgtta acgaatcatt
    13561 aaatgccgag tgggaaaata tcatggcctt gttcttgcca actggtgagt tgctgctgtt
    13621 gggcggaggt tcgattttca ccgcaccaca ccagcaatgt acggccttcg aatagttcag
    13681 ggcgtagttg attgagcgag tgggcgagga catcaatgcg ccatccttgt tgactggcaa
    13741 tccagccctc cagccacaga cgggtggtat cctgaatatt ccagccaacc accagcgcat
    13801 ctttaccctg ttttttacgt gccgaagcca gacaaatggc gatgtagttg atcagtacgc
    13861 cgtcgaggat cgccagcagc gcctggagag tcggttgttg gcattgaagt cgtcggcgca
    13921 gaggaataaa cagatgtgtg gtgagtgtct gggcggggta atcctgaccg cgctctttga
    13981 tccacgttcg caggctatgc agattgccgc tttgcaggta ggtcagtaat gtttcttgct
    14041 gatcgcgcca gccgttctgc acatcaacat tttcattact gagcagcatt ttaactttgc
    14101 tgacctgcac gccgttgtcg atccagcgtt tgatctcgcg gatacggtca atatcggcat
    14161 cgttgaacag tcgatgaccg ccgtctgtcc gttgcggttt cagcaacccg taacgcctct
    14221 gccacgcgcg taacgtgaca gggttaatat cacaaagcaa cgccacttca ccaattgtgt
    14281 aaagcgccat cgtctcaccc ttgctcgcga ggtcccggtt taactttaga cgcagttttg
    14341 cgaaccaggt agttttgccc gttttttgtg catctatagg gtgattttat ttttgccagg
    14401 cgattttgag tgatcgtact cacgaattct catttttctg caagagttca aagaaagtta
    14461 aacgcaggca atgtatgtta cgcgttttaa agggaagtgt ggtttgcggg tatgtacgat
    14521 tttaatctgg tgttgctgct gcttcagcag atgtgcgttt ttttagtcat tgcgtggtta
    14581 atgagtaaaa cgccattatt cataccgtta atgcaggtca cggttcgtct gccgcataaa
    14641 tttctctgct acatcgtctt ttccatcttc tgcatcatgg gcacctggtt tgggttgcac
    14701 attgacgatt ctattgccaa tacccgtgcg ataggcgcgg taatgggcgg cttactcggc
    14761 ggtccggtcg tcggtgggct ggttggtctg accggcggct tacatcgata ttcgatgggg
    14821 ggcatgaccg cgctaagttg catgatctca accatcgttg aaggattgct cggcggcctg
    14881 gtacacagca tcctgatccg tcgcgggcgc actgataaag tctttaaccc cattaccgcc
    14941 ggtgccgtca cgttcgtcgc tgaaatggtg caaatgctga tcatccttgc gatcgcccga
    15001 ccttatgaag atgcggtgcg tctggtgagt aatattgctg caccaatgat ggtcaccaat
    15061 accgtcggcg cggcgctgtt tatgcgtata ttgctcgata aacgcgcgat gtttgaaaaa
    15121 tacacttctg ctttttctgc cactgcgctg aaagtggcag cctcgacgga aggcattttg
    15181 cgacaggggt ttaacgaagt gaacagcatg aaagtggctc aggtgctgta tcaggagctg
    15241 gatattggtg cagtcgcgat taccgatcga gagaaattgc tggcctttac cggaattggt
    15301 gacgaccacc atttacccgg caaaccgatt tcttcgactt acaccttaaa agcgattgaa
    15361 accggtgaag tggtctacgc tgatggcaac gaagtacctt atcgttgctc tttgcatccg
    15421 caatgcaaac tggggtcgac gctggtaatt ccgttgcgtg gcgaaaatca gcgcgtgatg
    15481 ggcaccatca aattgtatga agccaaaaac cgtttattca gttcaataaa ccgcacgctg
    15541 ggcgagggga ttgcgcaact gctttcggcg cagattcttg ccgggcaata tgagcggcaa
    15601 aaagcgatgc tcacccagtc agaaatcaaa ctgcttcacg cccaggtgaa tcctcatttt
    15661 ttgtttaatg cgcttaacac cattaaagcg gtgatccgcc gcgacagcga acaggccagc
    15721 cagctggtgc agtatctttc cacttttttc cgcaaaaact taaagcggcc ttcggagttt
    15781 gttactctcg ccgacgaaat tgaacatgtg aacgcttatc tgcaaattga aaaggcgcgc
    15841 ttccagtcgc ggttgcaggt caacattgct attccgcaag aattatccca gcagcaattg
    15901 cccgcgttta ccctgcaacc gatagtggaa aacgccatta aacatgggac atcacaactg
    15961 ctggatacag ggcgagtggc aatcagcgcc cgacgtgagg ggcaacattt gatgctggag
    16021 atcgaagaca atgccggttt gtatcaaccg gtaaccaatg ccagtgggct ggggatgaat
    16081 ctggtggata agcgtttacg tgaacggttt ggcgatgact atggaataag cgtcgcctgt
    16141 gagcctgata gttacacccg aataacgtta cgactaccat ggagggacga ggcatgatta
    16201 aagtcttaat tgtcgatgat gaaccgttag cacgggagaa cctgcgtgta tttttgcagg
    16261 agcagagcga tattgaaatc gttggagagt gttcaaacgc cgtggaaggg atcggcgcgg
    16321 tgcataaact gcgcccggat gtgctgtttc tcgatatcca gatgccgcgc atcagtggtc
    16381 tggaaatggt ggggatgctc gagccggaac atcgtccgta tattgttttt ctcaccgcgt
    16441 ttgacgaata cgctattaaa gcctttgaag aacatgcctt tgattatctg ctgaagccaa
    16501 ttgatgaagc gcgactggag aaaacgctgg cgcgtttgcg tcaggaacgc agcaagcagg
    16561 atgtttcgct gttaccggaa aatcaacagg cgctgaaatt tatcccttgt acggggcata
    16621 gtcggattta tttgctgcaa atgaaagatg tggcatttgt cagcagtcgg atgagcggtg
    16681 tctacgttac cagccacgaa gggaaagagg gctttaccga attgacatta cgtaccctgg
    16741 aaagtcgtac accactactg cgctgccatc gtcagtatct ggttaacctc gcgcatttac
    16801 aggagattcg tctggaagat aacggccagg ccgagttgat tttgcgtaat ggcttaaccg
    16861 tgccggtcag ccgccgttat ctgaaaagct taaaagaggc gattggcctg taaaagactg
    16921 ctaaaatggc tttttgcctc atcaacacct gaaggcctca tgctaagtaa cgatattctg
    16981 cgcagcgtgc gctacatttt gaaagccaat aataatgacc tggtgcgtat tctggcgctg
    17041 ggtaatgtcg aagccaccgc ggaacagatc gccgtctggc tacgtaaaga agacgaagag
    17101 ggttttcagc gttgtccgga cattgttttg tcgtcattcc tcaatggcct gatttatgaa
    17161 aaacgcggca aggatgagtc tgctccggca ctggagccgg aacgtcgcat taataacaac
    17221 atcgtgctga aaaaattacg catcgcgttt tcgctgaaaa ccgatgacat tctggcgatc
    17281 ctcaccgaac agcagttccg cgtttcgatg ccggaaatta cagcgatgat gcgtgcaccg
    17341 gatcataaaa acttccgcga atgcggcgat caatttttac gttattttct gcgtggactg
    17401 gcagcgcgcc agcatgtgaa aaaaggctaa aaaattggcg gtcatgttta gagcatgacc
    17461 gccaaccgat tatttcactt ctttaaaacc agcggctttc atcaccagtt ccatttgcgc
    17521 catagtgata ccttttttgg catcttcagc agaaacgttg attcctgaaa taccctgcag
    17581 ggctttaaaa tccacttttt ccatatcgat agtcacgttt tcctgcgcgt aggtatctgt
    17641 ataggttaat ttttcttcaa cacccgcgat gtttttgtat ttggcgctta acggctcaag
    17701 tgtcttggca gcgtcttctt tggtggttgc accaatagaa gcaaattgaa ttttggtttc
    17761 agaagattgc ttaagcacct tgtcaccttt gtagacatag gtaatggcaa tttcagtgcc
    17821 gttcagattg gcgctgaatt tcttcgattc ttctttgtca ccgcagccag caagagagaa
    17881 aaccagaaca gatgcaacaa cgagggaaaa cagcttattg aaagccttca tgtaaaactc
    17941 cattttattt aatcaagaaa ctggtgactc tcaccagggg ctatatagaa tatgcctaat
    18001 accgtgacgt gagcagtccg gaactggagt agaactctta gtaaaaagca ctatttcatc
    18061 cttgttgctg aagcatgggg aataattgtt cgcaaagtaa aacaccgtta ttcattgctt
    18121 ctacccgtgc ctcgctttct gtattacgaa attgtcccaa cacatgtgcc agccgataaa
    18181 aaccgaccgc ggagaggtca ttcgccagca actctgcctg actaatagcg ctctgttcct
    18241 gatagcgcca gccgttatgg agcagttgaa taagtaacgc ctggcagcgc atcagcaact
    18301 gatgagcggt agacggcaca ggcaaaacgc tggcagaagg tagcggtgcc acaggcgcag
    18361 tttccgcgtc cagcgcccag gcgcgggttt ttgtcatcat cacccgtggt tccagtgtca
    18421 attgcccatc aacaaaactg acaaagccag aaaccagact cacggggtcg tctgtttgtt
    18481 gcaaaagcgc cgccatgcgt tcaacggcaa aaggagaaca ggcagatgcc ggaagggata
    18541 acgtcaggag attatcttca ccttcgccgc tgattacctg cgcatccagc gtctggcggc
    18601 tgctatccca accgagcgaa atacactcag cgaccggcag aataaataag ttatcgacct
    18661 gattaagagg ccgtatgcag gcggggggac gctggcgtaa atattcccgc aaagccacaa
    18721 tgcccggctg gcgtaacggc gcgctcaaca tttgccaggc atcaggcgac agcggcacaa
    18781 cgctgcttaa gcggttgcgg gtagctaaca gcagctcgcc atcggcactg cgttttgctg
    18841 cttgtgaaac aatttgccca cccgccagtg cgccagcctg aaaactaaac agccgacgcg
    18901 tagctgccgg tgagttttcc tgttcacttc gcggccaact gcgcgaaagg tgcaaaatac
    18961 tgccggtgtc gggatcggta aaccagatgc gtaaaccata atgctcaata tcctgccagc
    19021 aacgcatacc taaagacacc agccgcagat gatcaagctt tgcttctccg gcaatgccag
    19081 agccaacgac cgtgcgccac ggcacaggag gaacttcacc aacactgtcg cgccgggcca
    19141 tctcttgtgc gcaatttaat cgactgttta atgccgcaag ctgacgtaag cattctccgg
    19201 catgatagtg gctggcgcga gtgtggaagg catcaacgct tgcccgtagc tgtcgtagtg
    19261 attcactcac ccagcgccag ttgcaggcct ctgccgcctg caatgcgcgg ttgaatgctg
    19321 cctcgtagtg aataagcggc tggctgatgc cgccaagcca taatgcctgg cttaattgct
    19381 gaacatattg acgacacgtt tggccttctt cgctggcaaa cggatcgtca gatgatgtga
    19441 cgtgttcgct gcgcatctgc cagattaaat ggttaaattc tgcttgctgc gctttggcct
    19501 cgacgaaggc ctgcacagcc agtacgacat gttcgcaaag tgtgccttca atacaatcac
    19561 aacgggcgaa acgaatactg ctacgggaat aaaaacgcac atcgctcatc ggtaagcggg
    19621 cagagggaat ttcgcccggc gcacagaaca actcaatggt gatgccttta gcgaccagcg
    19681 cctgcgcgcg tttgcgggtg gcatcgggca gggtagccag ttcttccagc cagattgccg
    19741 gatcccactc ttcttctttt tccgtgggct ggacggtggc acaaagtcgt tgataactta
    19801 acaccagcat cacgcgatga cggcacatgc cgctggcccc gcagctgcac tgagcctctt
    19861 tcagtgcctg gctgttcgcc agctgggtac ggacaccgtc actgaaggtg gcgattaaag
    19921 cgccgttctc atggctgatt tccgggacgt tgccattttc cagttcctta agactgcgct
    19981 taacaaaacc ggcattgctt aacgccgtca gtgcctgcgg tgtcagttct aataattccg
    20041 gacgtagtga attcatgact gaagattctc cgcaagccat gatgccagct cgcccggcgt
    20101 catggcggct atttgtgcgc cgacattaac cagcgcctgg gccgtatcgc ggtcatagca
    20161 aggcgttgcg gtgctatcga gcgctgccag tcccagcact ttgatgccgc tctggacaca
    20221 ctttttcacc tgatgcgtca gcaatgatga tgaaccccct tcgtaaaaat cgctcacgag
    20281 gataatgacg ctttttgctg gttgttcaat aagttgccga ccatactcca cggcactggc
    20341 gatattggtc ccgccgccca actgtacttt cattaataac tctaccggat cggcaacgtc
    20401 tgccgtgaga tcaaccacgc ttgtgtcaaa cgccaccaga tgggtacgaa tgccgggtaa
    20461 ctgccacaaa caggccgcca tcaccgcaga gtggatcacc gagtccacca tcgatccgct
    20521 ttgatcaacc agtaagacca gttgccattg ttcgctttgg cgtttaatgc ggctgttaaa
    20581 gcggggggat tcgatataca acttgccgtg ttgcgggtgc cagtgttgca ggttggcgcg
    20641 cagagtactt ttgaaatcaa agtttcgcgc cagtggaatt aatgagcggc gacggcgatc
    20701 gcggacacca gaaaaagcct gacgaacttc ctttgccagt cgcgccataa tttcttcaac
    20761 aacctggcgc actatctggc gggcggcagc cagtacttcg ggattcatca gatgtttggt
    20821 gtgcaaaacg gcgcgtagca ggctttccga aggctgcata cgttccagca cgtcgagatt
    20881 tgtcaccaca tcttcaatgc cgtagcgcag tacggcatcg ctttccagcc gctcaatcac
    20941 ctgttgcgga aacagcgtgt ggatactgtt gatccactca ggagtggtga gatttgagcc
    21001 acctaatcca ccagagcgtt caccacgctg gagccgttca ggatcgcgcc catacagcca
    21061 ctccagcgcg tgatctatct gccgggcgtt gtcatccagc ccacaaagcg tcgtttctgc
    21121 cgcttcgcca agaattaatc gccagcgttg tagctcacgg gtggtcagaa gatcgttcag
    21181 ttcagacatg gaaaactccc cagtgttgca gcgatgccag tgcctgctgt tcgagttgtt
    21241 gatgatgtgc aatggcttgc ggtggacaat gcaacggcat ttgcagtgcc gaaacgggaa
    21301 gttgcgccag ttgataatgc tcaagcacct gatgcgccag cgttccgcgt tctcgtggcg
    21361 gtagccaggc catcgccgcg cgtaaatcgg gcagggcatt gataaaattg gcatcactca
    21421 gttgattcaa atggctgctg aaaccggcga taaatgcagg ttgacaggcc agttgatgac
    21481 gggctaatgc cagcaaaccg tgcagcgcct cgccggattg tgccggggat aactgcgcca
    21541 gcatcgtcag cgccgcttcg gcactggcgt tgggatgttc cagacgcatt agtgcgccaa
    21601 gagccgcgcc gcgatccagc gccggggcat gaatcgcctg actgcgtcgc tccagtaagg
    21661 ctaccgccgc agaaagcgaa acgccggata aattaacgcc gctatgtaga tcgcgcagaa
    21721 tatggcaaag cgcttgccag ctatgcaggt gagcgtgaaa ctccttttca tccggtctgc
    21781 cgttagattc acacagccac agcgtgcgat cgatagccgc gcataacgtc gtttgtaata
    21841 tctgcgcgcc ttgcataccg ctaatttcat ccagccgcca taaggcatat agcacttcca
    21901 gcgcctggcc catttcggca aattgatttt cctgggcgat taataatgtg agttgctcca
    21961 gtaattgttg actgaaggac gccagacccg ctaacgccgc ctggctaaga catgtggtga
    22021 tactgccgat tccacccgcg tccagcatat cggcttctaa tttatggcgt gcggcttcct
    22081 gaagtgttgc accaaagcat gcggcctcaa tcaatgccgc atgctggcta agcgggcggg
    22141 ttaatttcca gtgttcttca ccgttgcctg caagtgtcag tgtacttcac tgctggcgta
    22201 caatcccagg gatctccagt attgccagcc gatgtaacac ctgactttgc gccagcccat
    22261 cgggggtaaa gcgatttaag gtaagctccg ccggaagaga gattgtggta cgttccagtt
    22321 cgcaggtgac atcttttggc agaggcggtt gtggcgtgga aggggcaagt ttaccgaatc
    22381 cgtcacccgc taatgtgtct atcaacgtta gcagaatcgg atcggtatcg ggatgaataa
    22441 cgccgcgata gctccacggc aacggtgcat tcagggcttc tttaatcaac gagcctgcta
    22501 tggcatccag ccagtcagtg cgtaacggta gtgtatgacc gcgcaactgt gccagtgcca
    22561 ttgcatgcag atgtgcggca gccatatccg cggtcgaagc aggcaagttg tgctggcgca
    22621 aacgggtgag aaccgttttt agcagttgtt caccggcctg ctgtaagccc cactgccagc
    22681 accagttttg ccagaccggg gcaggcatac cggaaaggta tcctgccagc acatcaaggc
    22741 gcttttcact gtagggcgtg agataacaac ctgtaatggc atctgccagc gagggcaatt
    22801 ccggcgtgtt aatgtcctgc gggcattcgc gccacatttt tgccagtgcc ggagcgtgcc
    22861 agccaccgca gacgactaac acgtcgccat tattttgctg caccgcccat gcaatccagc
    22921 gagccataaa ggcttcgcgc tgacgattga gtgcatcgcc ggggaaatcg ccccgcagtt
    22981 gggcaaaata gtgcgccagc gcagagggta atgcagtttg ctggctttca tcttcgaaca
    23041 agtgatccca cagggtatcg ctgttatcca tgcgggtggc acgcagcagt aaggcctggc
    23101 tttcatcttg cgtatcaggc gaatcgtcct cttcttcact ctgcgcccag caaggcaaat
    23161 cgatgaagta agtttgtgcc tgaatacgac gtgcggcttg tagcgcctgc cactccggcg
    23221 aaaattcagc aaatggcgtc caggcaccac gcccgggggc tgcaccgtcc tgatactggc
    23281 aataactgta aatagctacc ggaagctggt gggaaaaaaa aagttcgtct acccggtcat
    23341 taaaatcagc cgggccttca atcaacacgt atcgtggccg ctggctttcg attaacgatt
    23401 tcaccagccg ggcgcaggcc ggactatgat gccggatgcc gacaattaac ggctcgctca
    23461 tgcctctttc ctcacggcag gcggtggcgg gcttgataat aagcctgcca gtgagcttct
    23521 ttatgtgtgg cgacacgctg ttcgaagtaa cggcgcagac gagcgcgatc ttcttcgtta
    23581 tctttgacga tggttccggc aatacagtcc accaggtcgg caggctcccc cgcgcgattc
    23641 gctaaaaacc aggcgcggac gcctacggcg tgggccacgt tcacggcttc agcggtggac
    23701 ataatcgctg tcagtgtatc cattgacgtt ttcttttcgc cgttggcacg cagatcgcgg
    23761 aaggtgcgta ccagtaactc cagtactgcg tcaggcactt tatgcggaat accgctatgg
    23821 gccagcaaac gcgccgaagc actggcgacc agttccagct cctgggcgaa atccataatc
    23881 ggaaagacgg tttcaaaatc gaagcgacgc tttagcgcgg cactcatctc attgacgccg
    23941 cgatcgcggg tattggcagt ggcaataata ttaaagcctt cccgcgcata gagctgactg
    24001 gcttcaccgg tgagttccgg caccgtcatc accctgtcag aaagcatacc taacagacag
    24061 tcttgtactt ccagcggtgt acgcgttatc tcttcaaaac ggacgatttt gccgtcgcgc
    24121 attccctggt aaagcggggc agggaccagt gcttccgttg acggaccatg gttgataagc
    24181 agggcgtagt tccagccgta cttgatttga tcttcggtag tagatgcgcc gccctggatg
    24241 gttaacccgg catcgccgct aatagcggtt gccagtaatt cagacaggag agattttgcg
    24301 gtgccaggtt cccccaccaa catcaaaccg cgcccggtgg caagagtgac taacatgcgt
    24361 tcgacagcgg agggactaat caccacttta cggctaatat tttgcgcgct atcgccaagg
    24421 ataaaagcac gggccgcagg caaacttaac tgccaaccgg gcgggcaagg tgcgttatca
    24481 ttttgtttta attttgccag ttcatcggcg tataacacag cagcaggcgg acgctgcaga
    24541 tgattattct gcggtgacat atattcatcc ctgaaatgta ttaatgaaat aaacgatatt
    24601 ttacgcgcaa taaatgtttg gcgcattagt tgtttgagcg aaaaatatct ggaaagcaga
    24661 tcacagtaaa taaaaaaacc tgctggtgag gcaggttctt ttatttacta ctttgatgtt
    24721 aagttactga tagtgcaaca tggcatctca acgaacgaat tataaccacc tctggacagt
    24781 gttgcaatta agataatgag atttataatc ctgactgttg ggaagttgcg ccggatcgaa
    24841 cccatttcaa gtcctggcta ccggacgggt ggtgccgcag gtagtgctct gatccggagt
    24901 gcaacatggc gactcaacaa acgtaaacca gtatcgacag gcagtgtaag ggtaaggtgc
    24961 tggtgttgcg ctccttaata aggagagcgg atggacacgc tgacacaaaa gttaaccgtg
    25021 ctcattgccg tactggagct attagtgtct ctgttacggt tgattgattt gttgaagtaa
    25081 tgggcaaatt gaaacgcaac taagggggaa aaccgcttcc ccctttaata aactaatatt
    25141 ctattagttg gttgaatata ttctgtagtt cacgattaaa aattgctatt acagtttcag
    25201 gtgattcacc tcgtaatttc cgctcatcaa tacactcatc tcttatataa atcaacgcat
    25261 catttattag cgggtcagca tcttctcgat cacgttgatg aaatgttgga cgtagccgga
    25321 atcgctccag tcgatagacg gaacctgtaa ataattcaga gcatttatgg atctcaagtc
    25381 tgaaggaaaa cggatcttca ggtacaggga cctcataatc gtaaatagca accagctctt
    25441 tttgcacgat catcgctttc cccgctggta agtcgtgttg ataacgggca gttgcatcac
    25501 tgcccgctgt gttttaatgg aaaatgacgt caggtgcgct taacgcctca ctcaaatcaa
    25561 acacatcaag ttcggcaaaa gtgacggatt tattgtcact gtacactcga agagaacgga
    25621 agctcattag cgctttgcta tcaacattgt agtggttaaa accgggtgaa aacttaagga
    25681 ccagccgcta ctcacccagg tcatagacaa atgcgtcctc atagcctgac tcccagcctt
    25741 tgtatcgcat tcccataagt tgcccaacgg tagcagattt accttcccag cgattcaaac
    25801 tattactggc tgactcgtca ggcgtgagca gcaccgtgcg ccgtgtcaac tggcgaagtg
    25861 gcggtataat ttcgtaatcg gcaaaaagct gggcaaattc actgcgcatt tctgctgtca
    25921 tttctaacgg gtgggcaatg ccaatcagag catccgcagg caggtcaatt ggctcatcct
    25981 gcgcattgca gaactccccc tctgcggcca cacgaaaggc gttgagtaaa cgacgcggtt
    26041 catttgccgg atacacgccc catattaatc gctgggtaac caggcgggta aagggatgat
    26101 taacaaacag agtatgaaaa tctgccagcg accagcgtcg ggtggtacgt agggcagctt
    26161 caagacgcgg gagcaggttt ttgctcacct gagtagcatc tttttttagc ccttttagtc
    26221 gggccagtgc ctcgggcgct ttcagttgat cgtcatcggc gcgcaaccgg ggaacgcttt
    26281 tctggcgaac gttttgctga tcaaagataa ccgggttgag gttttcatcg aagcgaacgg
    26341 taaactgccg gggaccaaaa tcaaaactca acgcctgcgg atcatcaagg cccagggttg
    26401 ggactaatct gtccgctagc tcttcctggc ttagcccgcg attttctgcg accacctgaa
    26461 gaaattccgc cgcgttatca cgtaaggggc gggatttagc ccgttgcgat atatgatgca
    26521 gctgtatcag cgccatatca ttattcatca gggtaaggat gttcaggccg ctgacagcac
    26581 gggcagattt gccttcttgt ggccaggcga ggatctgtgt cgtcagatcc cgtgccgtgc
    26641 tttcgtcacc aaagagactt aacgccagaa atgcccagtt gtcttttgcg ggggcaccag
    26701 cttgttgcca cgcagtgaag agatcccagg caaaagctgc cagcgtttgt ggctggcaga
    26761 acgttttcag ttgttccagc ccgctataaa aacgtccccc ctgggtaaag cgcagcattt
    26821 cgccgataat ttccagagcg tcaccggtaa caggctgatt attagtgata agtcgaggcc
    26881 tggaccacat tgccggatgc cagaaatcag gggcttttgg aatgcgtgcc gggtaaatgt
    26941 ccattggacc ctgtttaagc aactgctcca gggcagacca tacatctgta cgctgccagc
    27001 ggtttgccac ggtttgtagc aattcgccat gtccctgttc gtacagtaaa cgcagggcaa
    27061 gtaatgcggc ttcgctatta tcgctgggtt tggtaaagac aagagggata agtgcactag
    27121 ccgtatgttc cggccattgt aaaatccact ggcgagccag atcacgctgc gccgcgaaac
    27181 gtcgccagac gcgggcaaca ggcagcgcca gttctgttgc gccaaaattt aaaattaacg
    27241 gaaatgtttc ttttggacga tgtgaaaaag ccaacagcag acctggtagc gcatccaatc
    27301 cgaggatact gagaaaatag tcctcacccg cgaacagatg ctttttctca atgatacgca
    27361 gccagaaggt caaagccatc tcacgcggca actgtgccaa taaatacagg ttccagacac
    27421 tcttttccgg acgataacgt gagaagttgt cattttccca tatgttaatt aacgcttcta
    27481 tgtgaccgct gcggagcgcc tgttctgcta attgtgcttg cggggagttc cagccattca
    27541 ggatctcttc aacccgcccc tggtagtaat gccactgaac aatgagtgaa ttgcgtgcag
    27601 catccagttt tccggggagt atataattca gaaacagcca tacatcatcc ccaaaaccca
    27661 gttcctgtaa tacaactcgt ccactttttt gttccatgcg ttccgcaaga gattccgtgc
    27721 tgaaataacg ggcagactca gtggcggcaa gcctctcttg ttcgcctggt tgccagtaaa
    27781 tttgcggagt taattcgagc ggtgccaaat caagacgtgg agctgtcatt tttttcttac
    27841 tacgccacgg tggtgaaacg agcatttcag gcagactgct gttatcggtg ctatcaataa
    27901 ccggttttgg cggtgccgat aaataactat tcagcactgc cctggcttga gtacttaacc
    27961 acggcatcac cctttctgct agttgtggct gcgatgctat cagcgtttgt aacagcgtac
    28021 gccagtattc atcgcgtgct ttttgctgtg caggcagctt tttgtcttcg atgattggat
    28081 aacctgggcg ggctggtggt tcttttaacg ccagcagttc tgccagtgcg gcgagtgtcg
    28141 catgggggaa gtttttactg tatttagcca cacgttgcag gctgggtttg tttttatcag
    28201 caactaacag caaggtgcgg atgacttgcg gatggttaat ttgtaccagc agactgccgc
    28261 aatcttcttt atgggcatat atcgcgaggc gcggaacggc tgccagtcct tgttcgcgca
    28321 aaagggcggc gcaggccgcg tagccgaagt ggttttcatg cgacatgtag ctggcttctc
    28381 gatcgctaaa tacgtccagg ccccagcatc gttcgagttt ttttaccgcc gtattatcag
    28441 tagcaatgac ctttaaccac tcctttgaat ggagacttga ccgggagctt tcaagactgg
    28501 cgagttcatt ggttatttca ggtttttccg ggaggagtag tgcaataaaa ggacggcgaa
    28561 ttttgggtat tcctggcagt gcggcaatga gtttgtcggc gcaacgctgc cagctgcttt
    28621 cctctgccag agagagatgc ttacgcagtc ggagatcgaa ttcgttatag gtttctgagc
    28681 gataaccata gccataatcc tcgtcctgat actgcaatgt cactagcaga ggatcactct
    28741 cataacatcg tgtaataaac tggcgtgcaa tcaccacctc agtcgcatat tccagtccct
    28801 cttgttggac gatttcgtcg agcaactgtt ggctgtcatt ttgcgcgata cagactagcg
    28861 ccgccattgt ccgggccggg attgagggtt tgtgttcgcc gatcaagttg atggcctgcg
    28921 taatggcgga ctgccactcc agtgaaaaat tctcataacg ccagacaatg ttttttttct
    28981 tctcagcttt ttgtagcaat tcatcaccca gtgtctgaat gatgctttca gtgataacag
    29041 gcactggttc gcccggccaa ttgcgatggc tgagtggtgt tttgcctttt ttatatttca
    29101 gttccagttg ggcgttatcc gccagccacg gtaattcctt gtccatgctc tttccttatt
    29161 caaacagggc ttcaatatcg ttaatcagct cgctggcggt gatggcatcg agtacggaga
    29221 actgcgcttc ctgtgttgaa ttactccccc agccatagct ttctgctttg ccttcccata
    29281 gccacagctt gcttaagagt tgctcggcgc taagttcggc tggcgacata ccaactgcaa
    29341 agccttcatc gatttccatg attaacgacc agcggcccag aggtttgatc atccagccga
    29401 tccagccacc atcctgcggt tcaccacgca cccaaccttt attcgccagt cccatgaccc
    29461 gaccgctcgg gcattttctg cctgcccagc gggtcagttc actggcattg cgctcggctt
    29521 ctgtcagggc gtagctgtta cggtcgagct ggcggaacgg tggtagcagt tcgtagtcgg
    29581 caaaaagctg accaaaggcg gcagcatccg ttggtgatat ttccagaacg tgaggagtgc
    29641 cgatagagat atcgccttcc ggcagggtga aaagatcatc gtcagcggtg ctgtagctgt
    29701 tatcttctgc tacgcgaaag caagccagta gctggttttc ggcgctataa acgccccaaa
    29761 tcagacggcg ggttaagtga cgaaccagcg gatgctcaac caggaagagc tggaagtttt
    29821 ccagcgacca gcggcggcgc aggcacatgg cggattccag ccttgctacc tgctgggcgg
    29881 cgatggtacg cgcatctttt ttcagcaatt tgtagcggtt aaccgcatcg ttcgcccgcg
    29941 tttcatcatc gcttttgttc ggtttgggca ggtctttcag gcggctgccg gaaacatcac
    30001 gcacaaacgg ttttaaggtt tcatcaaagc tgacggtgaa ctgccgtggg ccaaaatcca
    30061 gcagcagcga accgttatca tccagaccga gatccggtgc taaccgatct tcaagctccg
    30121 ccaccgtgag ttcgcggctc tcggcaatgt cggcaatttt ttcttttgcc cgctcctgta
    30181 atgctttgaa tttcagtttc tgggcgatgc cgttaagctg cataagggcg atatcactac
    30241 cgatagcagc gagaatatcc aacccaacgg tggcgcgttt atgctgggat tcaccaggcc
    30301 aggcgcgtat taatggcgtc agtttgcggg cggtgtcatc gttaccgaga acgcctaacg
    30361 cagtgaacgc ccaactctct ttcgacggcg cgccagcggt ctgccaggcg gtaaacagat
    30421 cccaggcaaa tcccgccagt gagtcggcgg aacagacgtc tttcacctgc aataatcccg
    30481 gatacagagc ctcttcctga gggaagcgga gcatttcacc gaggtggagg agggcgctat
    30541 ctggcagtga ttgggcattt gcttttaata ccgggcgcgt ccagagcgat ggctgataaa
    30601 aggccggaag agtggggatt ttgtcgggtg attatctaag ggatcgagcg caagcagagc
    30661 gttcaccgca tcggttactt ccggctggtt ataacgtcgg gcgatttctt gcagtaatgg
    30721 ctgatgaccg ttttcggtaa gcatacgcaa ggcagcgcgg gcgttatcct gtgcttcacc
    30781 ggctttgccg agcgccgcag gcagcaggcc ggtaagggca tgttccgggt atttcaacag
    30841 ccagctacgg gcgttttcgc gtagcgtttt cagtttgttg aaggcacggg cgacggcagg
    30901 agccagctca ctcgctgcga agtaattcgt gatgggcaag gcttcttgtg gatagcgtgc
    30961 aagtgagttt actaacccag gtaacccttt gagtccaaaa tgtaacatgg cgtattcggt
    31021 atcggtatgt ggagataggg taagggcatt ccatgctttt actgctttct ccagcggcaa
    31081 tgttgtcaaa atccccaact gccaataatg cccggtaggt gaatgaaaca ccttaaattc
    31141 acttagtaag gttgaataat cttcatttag ccatgcctca actaccgctt cgggagcttt
    31201 aatatatgta ccagcgatcc gtcgttgaaa acccatcctt gccaacaggt tggtagtact
    31261 ttcttcatgg ctaaccgtga tgtgttttga ataccagata tatttcgcca aaagttgatt
    31321 actgatttct tctgtcagat aacagattgg ctcaatgcct aatggcgcta aatccagcac
    31381 cggaatcggc gattttttct ttttcgaaag ccagggaggg gagactacta cggctggcag
    31441 tagatcggcg ctggcatggt ttgagggctg tgtcagttgt tgctggcatg atttcagtac
    31501 ggcaactgcg ggtgtcgaga gccagggaat gacctgttct gccagtgctg gttgtgagat
    31561 aagcattgtc attagcataa tgcgccaact gttctcttct ttttgcccaa gaagttccgt
    31621 cagtgccgcc attgctgcat gtgggaacgc agcgcaggct ttcgtcatcc gatcgtgaca
    31681 gcgtttagta tgcccggcta cacgtataag cagtgttaat gcgaacggat gattgatatg
    31741 acgcaacaca tcggcgcagt agtcacttgc ggcataggga gcaaatcggg gcagggctgc
    31801 aacaccttgc tcctgtaata cggtggctga ccatatcgca ccacaatagt aatcatcaaa
    31861 aatggcatat ggctcgtgat atttttctaa tgaggcaaga atgtgctcat cagtcgccac
    31921 tatttttaac cactcaagcg agggtaattt tttttgcccc agtagacgct gggcaatttc
    31981 atgtgcaatt tctggttttt cgggtaataa caaagcaatt aatggttgcc gccattctgg
    32041 aatatgtgga attgcggcaa taagtttttg tgcacatttt tgccagagag actcttccgc
    32101 cagtgataaa tgtttacgta ggcgtaattc aaatctggaa tattgctcca agtagtcagg
    32161 tgcaatgcca gacggcagaa taataatgac attattcgca taatcccatt taatatcaat
    32221 ctgttgaagg tgtattatta catcagtagc atattccaga ccgccttcct ggacgatctg
    32281 atccataaca tcctcacgca ctttatatct tgtgcatttc atttcaagtg ccgtgagtac
    32341 agccattact ggtgatggta gctgtgttgt tttcaggccg tcgatctgac tgatcgcctg
    32401 ggcgacggtg ttttgccatt ctaaagaaca ggcgctgaag tcaaatgtgg ctgttttatt
    32461 atcccgttgc gacacactac aggctaagcg acgtaattgg gtcaatttgt ctgcttgctg
    32521 aacaggaact ccgggaaagc ggcgatgact taacgtggtg tgtacaactt cgggcggaaa
    32581 aacgacagca atatctttcg ccagccacgg taaaacagca tctgtatttt tgtcaggcgc
    32641 acgggtttct gctacgaaag agggttgttc ctgaataatg gtggataagt ccgctacctt
    32701 gagagagccc gttaccgttt gggaaggttg caaagaatta tccttcgctt gctccacata
    32761 ccccttcttc accttctccg caatcagctt aagctccgct tttgccactg ccgcagcatc
    32821 tgaaaaactt tttatctggc tttgcccttt ggtgccaact tttccccaac tgatatgcaa
    32881 ctcgtttccc tgttgctcaa ctgcccagaa tttatgtgat ttttcgtcct gatagataaa
    32941 gtgtctcatt ctgcatccct gtaattttat ccagttgaat gcagatgcta ccagtattta
    33001 tgcgggttag agagagacaa aatcgcaaga aaacaacttt aggaatttat aaagaaaagg
    33061 cgctgtcgat gcagcgcctt aaagggggat tatttcacct gatggcctgg tttagcacca
    33121 gcatccgggc ttaacaggaa gatatctttc ccgccaggac cagcagccat taccatgcct
    33181 tcggagatac caaagcgcat tttacgcggt gccaggttgg ccaccataat ggtgtgacgc
    33241 ccgatcagtg cctgcggatc cgggtaggca gaacgaatac cggagaagac attgcgtttt
    33301 tcaccgccga gatccagtgt caggcgcagc agtttgtcag aaccttcaac aaactctgcg
    33361 ttttcgatca gcgccacgcg caggtcaact tttgcgaagt cgtcaaaggt gatggtttcc
    33421 tgaatcggat catctgccag tgggccagta actggcgcgg cagctgcttt cacttcttct
    33481 ttagacgctt ccaccagtgc ttcaacctgc ttcatatcga tgcggttata cagcgctttg
    33541 aacggattca ctttgtggcc cagcagcggt tgctggatac catcccaggt cagttctgta
    33601 ttgaggaatg cttctgcgcg ctcggtcagt ttcggcagta ccggcttcag gtaagtcatc
    33661 agcacgcgga acaggtttat gcccattgag caaattgcct gcaggtcggc atcgcggcct
    33721 tcctgttttg ccaccaccca cggagcctgc tcgtcgacat agcggttagc cagatcagcc
    33781 agcgccatga tttcgcgcac tgctttacca aattcgcggc tttcccacgc ttcaccaatc
    33841 acttcagcgg catcagtgaa ggttttgtac aactgcgggt cagccaattc gcttgccagc
    33901 acgccgtcaa aacgcttgtt gataaagccc gcattacgag aggccaggtt aaccactttg
    33961 ttcacgatat cggcattcac acgctgaacg aaatcctcca ggttgagatc gatatcatca
    34021 atgcgcgaag agagtttcgc agtgtagtag taacgcaggc tgtcagcgtc aaaatgattc
    34081 agccaggtgc tggctttaat aaaggtgccg cgagacttgg acatctttgc gccgttcacc
    34141 gtcacatagc catgaacaaa caggttggtc ggcttgcgga agttgctgcc ttccagcatg
    34201 gcaggccaga acaggctgtg gaagtaaaca atatctttac cgatgaagtg gtacagctcg
    34261 gcggtggagt ctttcttcca gtattcatcg aagcttacgc tgtcgccgcg cttgtcgcac
    34321 agattcttga aagaacccat gtagccaatc ggtgcgtcca gccagacgta gaaatatttg
    34381 cccggcgcgt tcggaatttc aaaaccaaag tatggcgcgt cgcgggagat atcccactgt
    34441 tgcaggccgg attcaaacca ctcctgcatt ttatttgcca cctgctcctg caacgcaccg
    34501 ctgcgggtcc atgcctgcaa catttcgctg aaagagggca gatcaaagaa gaagtgttca
    34561 gaatcacgca ttaccggcgt agcgccagaa accaccgatt tcggctcgat caattcagtc
    34621 gggctgtagg tcgcgccgca gacttcgcag ttatcgccgt attgatccgg ggatttacat
    34681 ttcgggcagg tgcctttcac aaaacggtcc ggcaggaaca tgcctttttc cggatcgtac
    34741 agctgagaga tggtgcggtt tttaataaaa ccgttctctt tcaggcgaga gtagataagt
    34801 tctgacaact ggcggttctc ttcgctgtgc gtcgagtgat agttgtcata gctgatgtta
    34861 aagcctgcga aatcagtctg atgctcctga ctcatttcgc caatcatctg ctccggggtg
    34921 ataccaagct gctgagcttt cagcatgatc ggtgtaccgt gggcatcgtc ggcgcagatg
    34981 aagttgacct cgtggccgcg cattcgctgg taacggaccc agacatcagc ctggatgtgc
    35041 tccagcatat ggccgaggtg gattgagccg ttagcgtacg gcagtgcgca cgtcaccaga
    35101 attttcttcg cgacttgagt catagtaggc attacttctt aatagtgaaa aggggttaga
    35161 tagtaccaaa tgggaaaatg ttaagtaagt acttctctat attcttcagg taaactatca
    35221 gctgatggtt tatctataca aaaaaggagt cgggatgaac gaacaatccc aggccaaatc
    35281 accagaagcc ttgcgcgcaa tggtcgccgg gacactggcc aattttcagc acccaaccct
    35341 gaagcataac ctcaccacgc tgaaagcgtt gcaccatgtc gcctggatgg acgacacgtt
    35401 gcatgtggaa ctggttatgc cattcgtctg gcatagcgcg tttgaagagt taaaagagca
    35461 atgtagcgcc gaactgctgc gtatcaccgg ggcgaaagct atcgactgga aactgtcgca
    35521 caatatcgcc accttaaaac gcgtgaagaa tcagccgggt attaatggtg tgaagaacat
    35581 cattgcggtc agctccggta aaggcggcgt gggtaaatcc tccacggcgg taaacctggc
    35641 gctggcactg gcggcggaag gggcgaaagt cggcattctg gacgccgata tctacggccc
    35701 atcaattccc accatgctgg gcgcagaaaa tcagcgtcca acgtcgccgg acggtaccca
    35761 catggcaccg attatgtctc atggcctggc aaccaattca attggttatc tggtcactga
    35821 cgacaatgcg atggtgtggc gtggaccgat ggcgagcaag gcgttaatgc agatgttgca
    35881 ggaaaccttg tggccggatc tcgactatct ggtgcttgat atgccgccag gcaccggtga
    35941 cattcaattg acgctggcgc aaaacattcc agtaactggt gcagtcgtgg tcaccacccc
    36001 gcaggacatc gcgttgatcg atgcgaaaaa aggcattgtg atgttcgaga aagtcgaggt
    36061 tccggtgctt ggtattgtcg agaacatgag tgtgcatatt tgcagtaact gtggtcacca
    36121 tgaaccgatt ttcggtaccg gtggagctga gaaactggct gaaaaatacc acacacagct
    36181 gctggggcag atgccgttac acatctcctt acgtgaggat ctcgataaag gcacgccaac
    36241 ggttatcagc cgtccggaga gcgaatttac cgctatctac cgccagctgg ctgaccgcgt
    36301 tgcagctcag ctctactggc agggtgaagt cattccaggc gagatttcct tccgcgcggt
    36361 ctaacgccta ttaaccattc tggttattct tcatacaccg cagagatgtt acattgatgc
    36421 ggtgtattag tttttgccgc attaatgaac acttatttaa tactgcgaat attctgcgga
    36481 ccttcgtccg aaactctaca aagtcgattg atcatacagg caatgcttca ttatcatcct
    36541 tcgtggctgt gtagtggatg tacagctata gtttgttatg cactcattct atccaggcat
    36601 aacctttact acagggataa cgttcaatga ataagtattg gctgtcagga attatttttc
    36661 ttgcctatgg attggcttct cccgcattct cttcagagac tgccacatta gcgattaatg
    36721 gcaggatcag tcccccaaca tgcagtatgg ctatggttaa tggtcaacct caacagcatt
    36781 gtggtcagtt aacgtataat gttgacaccc gtcatctgtt ttcttcgccc gtcaaaggtg
    36841 taacgacaga agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct
    36901 acgattaatt tggcaacctg attttaattg catttagaaa gccgaaatca tttatataaa
    36961 tgatttcggc ttttttattg atatcaacaa tac
//
LOCUS       DAZGVQ010000031        36575 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000031 DAZGVQ010000000
VERSION     DAZGVQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36575
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.031"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..611
                     /locus_tag="ACHLAG_001515"
                     /pseudo
     CDS             1..611
                     /locus_tag="ACHLAG_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(647..1603)
                     /gene="ybhN"
                     /locus_tag="ACHLAG_001516"
     CDS             complement(647..1603)
                     /gene="ybhN"
                     /locus_tag="ACHLAG_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFP0654770.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1603..2844)
                     /gene="clsB"
                     /locus_tag="ACHLAG_001517"
     CDS             complement(1603..2844)
                     /gene="clsB"
                     /locus_tag="ACHLAG_001517"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFP0654771.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2841..3602)
                     /gene="ybhP"
                     /locus_tag="ACHLAG_001518"
     CDS             complement(2841..3602)
                     /gene="ybhP"
                     /locus_tag="ACHLAG_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP0654772.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRVHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3735..4145
                     /gene="ybhQ"
                     /locus_tag="ACHLAG_001519"
     CDS             3735..4145
                     /gene="ybhQ"
                     /locus_tag="ACHLAG_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFP0654773.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(4107..5213)
                     /gene="ybhR"
                     /locus_tag="ACHLAG_001520"
     CDS             complement(4107..5213)
                     /gene="ybhR"
                     /locus_tag="ACHLAG_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0654774.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5224..6357)
                     /gene="ybhS"
                     /locus_tag="ACHLAG_001521"
     CDS             complement(5224..6357)
                     /gene="ybhS"
                     /locus_tag="ACHLAG_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0654775.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6350..8086)
                     /gene="ybhF"
                     /locus_tag="ACHLAG_001522"
     CDS             complement(6350..8086)
                     /gene="ybhF"
                     /locus_tag="ACHLAG_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP0654776.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHAEGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGIDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(8079..9074)
                     /gene="hlyD"
                     /locus_tag="ACHLAG_001523"
     CDS             complement(8079..9074)
                     /gene="hlyD"
                     /locus_tag="ACHLAG_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFP0654777.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(9077..9748)
                     /gene="cecR"
                     /locus_tag="ACHLAG_001524"
     CDS             complement(9077..9748)
                     /gene="cecR"
                     /locus_tag="ACHLAG_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFP0654778.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            9977..11341
                     /gene="rhlE"
                     /locus_tag="ACHLAG_001525"
     CDS             9977..11341
                     /gene="rhlE"
                     /locus_tag="ACHLAG_001525"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFP0654779.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            complement(11573..12055)
                     /gene="ybiA"
                     /locus_tag="ACHLAG_001526"
     CDS             complement(11573..12055)
                     /gene="ybiA"
                     /locus_tag="ACHLAG_001526"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFP0654780.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            12175..14325
                     /gene="dinG"
                     /locus_tag="ACHLAG_001527"
     CDS             12175..14325
                     /gene="dinG"
                     /locus_tag="ACHLAG_001527"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFP0654781.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAINDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            14353..15315
                     /gene="ybiB"
                     /locus_tag="ACHLAG_001528"
     CDS             14353..15315
                     /gene="ybiB"
                     /locus_tag="ACHLAG_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFP0654782.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            15456..16541
                     /gene="hcxB"
                     /locus_tag="ACHLAG_001529"
     CDS             15456..16541
                     /gene="hcxB"
                     /locus_tag="ACHLAG_001529"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFP0654783.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            16608..16667
                     /gene="ybiE"
                     /locus_tag="ACHLAG_001530"
     CDS             16608..16667
                     /gene="ybiE"
                     /locus_tag="ACHLAG_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFP0654784.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(16770..17030)
                     /gene="ybiJ"
                     /locus_tag="ACHLAG_001531"
     CDS             complement(16770..17030)
                     /gene="ybiJ"
                     /locus_tag="ACHLAG_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFP0654785.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(17295..17561)
                     /gene="ybiI"
                     /locus_tag="ACHLAG_001532"
     CDS             complement(17295..17561)
                     /gene="ybiI"
                     /locus_tag="ACHLAG_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFP0654786.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(17635..18312)
                     /gene="ybiX"
                     /locus_tag="ACHLAG_001533"
     CDS             complement(17635..18312)
                     /gene="ybiX"
                     /locus_tag="ACHLAG_001533"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFP0654787.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(18354..20636)
                     /gene="fiu"
                     /locus_tag="ACHLAG_001534"
     CDS             complement(18354..20636)
                     /gene="fiu"
                     /locus_tag="ACHLAG_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFP0654788.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(20901..21161)
                     /gene="mcbA"
                     /locus_tag="ACHLAG_001535"
     CDS             complement(20901..21161)
                     /gene="mcbA"
                     /locus_tag="ACHLAG_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFP0654789.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            21437..22363
                     /gene="rlmF"
                     /locus_tag="ACHLAG_001536"
     CDS             21437..22363
                     /gene="rlmF"
                     /locus_tag="ACHLAG_001536"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFP0654790.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(22360..24585)
                     /gene="ybiO"
                     /locus_tag="ACHLAG_001537"
     CDS             complement(22360..24585)
                     /gene="ybiO"
                     /locus_tag="ACHLAG_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFP0654791.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(24702..25424)
                     /gene="glnQ"
                     /locus_tag="ACHLAG_001538"
     CDS             complement(24702..25424)
                     /gene="glnQ"
                     /locus_tag="ACHLAG_001538"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFP0654792.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(25421..26080)
                     /gene="glnP"
                     /locus_tag="ACHLAG_001539"
     CDS             complement(25421..26080)
                     /gene="glnP"
                     /locus_tag="ACHLAG_001539"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFP0654793.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(26219..26965)
                     /gene="glnH"
                     /locus_tag="ACHLAG_001540"
     CDS             complement(26219..26965)
                     /gene="glnH"
                     /locus_tag="ACHLAG_001540"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFP0654794.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(27369..27872)
                     /gene="dps"
                     /locus_tag="ACHLAG_001541"
                     /gene_synonym="pexB"
     CDS             complement(27369..27872)
                     /gene="dps"
                     /locus_tag="ACHLAG_001541"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFP0654795.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(28171..29058)
                     /gene="rhtA"
                     /locus_tag="ACHLAG_001542"
     CDS             complement(28171..29058)
                     /gene="rhtA"
                     /locus_tag="ACHLAG_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFP0654796.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            29293..29358
                     /locus_tag="ACHLAG_001543"
     CDS             29293..29358
                     /locus_tag="ACHLAG_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654797.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            29411..29926
                     /gene="ompX"
                     /locus_tag="ACHLAG_001544"
     CDS             29411..29926
                     /gene="ompX"
                     /locus_tag="ACHLAG_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFP0654798.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(29975..31558)
                     /gene="opgE"
                     /locus_tag="ACHLAG_001545"
     CDS             complement(29975..31558)
                     /gene="opgE"
                     /locus_tag="ACHLAG_001545"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP0654799.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGSIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(31830..31958)
                     /gene="mntS"
                     /locus_tag="ACHLAG_001546"
     CDS             complement(31830..31958)
                     /gene="mntS"
                     /locus_tag="ACHLAG_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFP0654800.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            32144..32611
                     /gene="mntR"
                     /locus_tag="ACHLAG_001547"
     CDS             32144..32611
                     /gene="mntR"
                     /locus_tag="ACHLAG_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFP0654801.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            32608..33726
                     /gene="ybiR"
                     /locus_tag="ACHLAG_001548"
     CDS             32608..33726
                     /gene="ybiR"
                     /locus_tag="ACHLAG_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFP0654802.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(33785..34705)
                     /gene="ldtB"
                     /locus_tag="ACHLAG_001549"
     CDS             complement(33785..34705)
                     /gene="ldtB"
                     /locus_tag="ACHLAG_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP0654803.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            34924..36516
                     /gene="ybiT"
                     /locus_tag="ACHLAG_001550"
     CDS             34924..36516
                     /gene="ybiT"
                     /locus_tag="ACHLAG_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFP0654804.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
BASE COUNT         8767 a         9312 c         9735 g         8761 t
ORIGIN      
        1 gcaggactgg cctggggagc taagtatctc gaaattacgg caaccaaata tgattcacca
       61 acaatgtatg tcgccatagg gttattatta gtttgtatgt atggcttagt aaggatatca
      121 acaagataaa tgccgtcatc gcaggcgtaa tatatctgtt tttactctct ttggtggcga
      181 ttgtcgttgc aagtttagct cctgtacctg ccattatcgt cgtgttcagc actgcgggcg
      241 cgatgttttt aatcagtatg ctggccggtt tattatttaa tgttgatcct ggttctcacc
      301 gttttatcat tatgatgacg ttgacagggt tggccctggt gatcatcatg aatgccgcat
      361 taatgagtga acgacccgtt tgggtaataa gttgcttaat gattgtgtta tggtcaggca
      421 ttatctcgca tggacgaaat aagctccttg aattggcggg gaaatgccat agtgaagagt
      481 tgtggagtcc ggttcgttgc gctgttacag gtgcattaac actctattac tattttatcg
      541 gcttctttgg gatacttgcc gcgatagcta taacgcttgt ctggcaaagg catacgcgtt
      601 tttttcatta gtcgtaaatg cagatactaa agaacttaac taaacttcac atcgccgctt
      661 catttttcgc ccgtagcttc ttcgcctggc tttccagtag caggtaacag atcagcgcca
      721 gcagcagcgg gataaagtaa tacaggacgc gataagcgag cagggcggcg ataatgctcc
      781 ctttcgaggt atgctcccca gccagtagtg cgataaacac cgcttccagc acaccgatcc
      841 ccgccggaat atgcacgatg acgccagcaa tactactaac cagtaacacg cccagtacaa
      901 agaaatagtt cacgctttga ccaagtaaca gccagataat cgcccccatt accatccagt
      961 taacgctgga aatcagcatc tgagcgaggg cgaatttcca tgaaggcagc accagctttt
     1021 gccctttgat ggtcatatgg cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga
     1081 taatcatcag taagccaatg ccgagaatac gcagcgtagt ttgatcgaca taccagtgat
     1141 ccggcaactc caccacgcct gcggtaaaga taatccctgc cagtaaaata tagcccagcc
     1201 agttggtggt aatactgagc gagaaaatcc gcgtaatagt gctgcccggt aaccctagtc
     1261 gggagtacaa acgatagcgc ataccaatgc cgccgaccca ggtactgagc gtcaggttga
     1321 aggcgtagca gataaacgac accagcatca cctggcgctt cgccagtttg tgaccgcagt
     1381 aaaagcgggc gagcaggtca tagcagccgt aaatcagata gctgacgacc accagcccga
     1441 ccgcactaag cagcgcaacg cgattgtagt cgcggatgac cttccagacc tcttcccagt
     1501 ccactttttt ggcgtagacc accagcaaca caatcaccgc gataaaaaac agccaggtga
     1561 ggatcttctt tgctaagcgc cagcgcgggt gtgatttact catcagggtt ttacccccgt
     1621 gttttcagtt tctacccgat cctgcgtttc cattgtcggt tgtgcgggcg gatcaacctg
     1681 cgccagacgt ggtgtgtgtg ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa
     1741 gtggaacgcc agcacgcttt tggtcaggtt ccaccaggtg cgtttcggca gcatggtctc
     1801 atcgacctgc tgacaatctg cggcaataat gccgttcaga ttatcgcgca gcgtctggtt
     1861 aaaatgacga tcgtggatga tgacatttgc ttcgagattc agtgacaaac tgagcggatc
     1921 gagattactg gaccctactg tcgcccagtg atcgtccatc aatgccactt tgccgtggag
     1981 cgggcggcgg cggtactcaa atacctgaac gccgccttta accagatagt tatacagcaa
     2041 gcgcgcaccg actctgacaa tcggcatatc cggttcgccc tgaatgatca gtttgatccg
     2101 caccccgcgc cgtgccgctt tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta
     2161 ggcgttggcg ataatgactt ctcgccgcgc ctgagtgagc attttcagat agtgacgttc
     2221 aatatcatcg cgatgttctt cgttatcgcg ccagaccagc aatacctgcg cttctcccgg
     2281 ctggcggttc tcttccgctt tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtcc
     2341 aggcaggttt tccagctcaa actggagaat atcttcgaca atcggccctt caaggcgtac
     2401 cgcgtaatcc tgtttagcct ctggaccgta gctggacata tgctcggcgg agtaattcag
     2461 cccgccaata aaggcgatac gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa
     2521 cacattggtg cgcataccaa aaaggcgagg gcggggatcg tagtagcgaa ataccacgcc
     2581 agctgccgtc agttcattga caaactcatc gctgagatcc ggcgaaccgt agccatccag
     2641 caagacttcc gctttaaccc cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt
     2701 gccgacgtca tcctcaaacc agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc
     2761 aatcgcctta aacaccgcgg gataatattg ctcgccgttt tccagcaact ggatcttatt
     2821 gccttcgcgc cagctacatt tcataaatga atctccgcac ttaaaggggc atgatcagaa
     2881 aggtgtcgcc atgtccgcag cggcaacgcg gttggcgcgc tggcgctggc atttttgacg
     2941 tagatcctgt ccagtcgtag tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg
     3001 tggacgcggg taaaaatctc atccagtccg gcctgcactt ttaacggatg attagctttt
     3061 tgccgccagt cattgaaatc acccgccacc aataccggtt cgccgtccgg tagctcattc
     3121 acccattcgg cgagcatcgc aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt
     3181 acgcacatca catgaatcgc ttttccggtc atcggtggca caatgcggca gtagagcacg
     3241 ccgcgctttt ccgcaccatc gaccgaaaca tcgcgattct cataatgttc aatgggataa
     3301 cgcgacagta cggcgttgcc gtgatgccct tccgggtata cggcattgcg accgtaggca
     3361 aaatcgctcc acatcgtgtc ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc
     3421 acatgcagcg gatgaacttc gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg
     3481 ctgacggtac gcaccgcgtc gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta
     3541 aaacctttgt gaatgttgat ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc
     3601 ataattttcc tgtctttgcc tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag
     3661 gtgcggtctt acggaatttt ccgtaaagtt cggtactctg agtaagtaga gataaattct
     3721 tcaggagaga agccatgaag tggcaacaac gtgttcgtgt cgcaactggt ctaagttgct
     3781 ggcagattat gttgcattta ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga
     3841 ctctggttca cgtcggcgtg ggattatgcg cactgtattg tgtcacggta gtgatgatgt
     3901 tggtgtttca gcgccacccc gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc
     3961 tgaccacgac ctggtatttt ggcgcagcgc tgattgtgct gtggctgttg tcccgtgtac
     4021 tggaaaacaa ctttttgctg gcaattgcag ggctggcaat ccttgccggc ccggcggtag
     4081 tgtctttgct ggcgaaagat aagaagttac atcaccttac gtctaaacat cgcgtacgcc
     4141 gctgaccctg tcgtggccgt tatcaccagt agcggccaca aactattcca cacaatatcc
     4201 agactcgcat ccttcaaata aatctgcttg gtaatgtccg taaagtggcg aatagggtta
     4261 atccacgtca ggttttgcag ccatactggc atgttttcca ccggagaaac gtaaccggaa
     4321 aggagaatgg cgggcatcat aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag
     4381 cagagtgatg aaatcaacag accgaatccc accagcgata aaccgtaaat caccatcgta
     4441 aagtagaaca gcgccagcga tccggcgaag gggatttgat acgcccagat accaatcgcc
     4501 agcacaatgg tggcctggaa ggtggcgaca attaacgccg gtacggcttt gccgatgaag
     4561 atctgccagg tggtgagcgg cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg
     4621 cgggcgacgg aaagtgaagt gacgatcatt acgccgatag tggtgatcat ggcgatcagc
     4681 gacggcacca caaaccattt gtagtcaaga ttcgggttat accagttgcg taccaccagc
     4741 tcgctgttgt taggtttcgg ttttccttcc agcagctcct gctgataatt tttgacgatc
     4801 tgttgcaggt agttggcggc aatttgcgca ctgttggagt tacgcccgtc gaggatcaac
     4861 tgcaacggcg cggtctggaa ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc
     4921 agtagtaacg ccttttgtgt gtcgatggtt gggcggatct cctgtgggct tttcagcagc
     4981 agcacatgag taaaggcgct ggcgcgggca aaacgttggg tcagctccac cgaatgctcg
     5041 ccgttatctt catcgtagat ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac
     5101 gggaacagaa tcacctgtat aagcacaggt aaaatcagaa tcgcgcgggt ttgcggttcg
     5161 cgcagcagcg actgcaactc tttgcggatt aacgtccata agcgatgaaa catgctcttc
     5221 tccctaatcc agccgacgtt tggttttcag ccacgtcagg ccgataaaca tcaccgccga
     5281 agcgatcaaa aacagcacgt ttaccaccag caccactgga atattcccgg cgaggaacag
     5341 gctttgcagg gtgctgacga aataacgagc gggaataatg tacgtcaccg cgcggatcac
     5401 cgcgggcata ctgtcgatct gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc
     5461 ggcgttcagg gcgacctgag cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag
     5521 ccccatcccc agggtactga gtaaaaacag gctggagata aaaaacagaa tcagcagcga
     5581 cccgcgatac ggcacgccga gaataaacac tgacaccagc atacacagca acatcgccag
     5641 catcccgaga aagtaataag ggatcagctt acacagcagc agttccgtgc gggtaatctc
     5701 cgtagagagc agagcctcca tggtgccgcg ttcccattct cgcgccacca ccagcgaggt
     5761 gagaatcgcg ccgatgaccg tcatgataat ggtcaccgca ccggggataa tgaagtgctg
     5821 gctaatcgcc gccgggttaa accagtagcg ggtttgtaca tcaataagag gttcaaaagt
     5881 ctgcccgttg tcctccgctc gctgcatttg ccagatctgc cagatccctt cgacataccc
     5941 ctgtacaaag ttagcggtat tcggctcact gccgtcggtg atcacctgaa tcggtgcggt
     6001 ggcgttggcg cgctccatct gttccgcaaa atccaccgga ataaccacca gaccgcgaat
     6061 tttccccgcc tgcattttgg cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat
     6121 gtagggcgaa ccggtcatgg tgtgggtgaa atccagcgcc gcttcgctac gctgttccag
     6181 taaaatcccg acccgcagct tgctggagtc gaggttaatg ccgtaaccaa aaataaacag
     6241 cagcaacagc gggatcacta ccgcaatcag ccaactactc ggatcgcgaa cgatctgccg
     6301 cgtctctttg acgcacagcg cccgcacgcg acgccaggaa aggatcgggt tactcattgc
     6361 tatgctcctt atcccagtcg tggatcaact gaataaaggc ttgctccatg gtgggatcgg
     6421 gttgctcatc gttagccgac tgtgctttca aatcgtccgg cgtgccgctg gcgattaatt
     6481 tcccgcggta caccaggccg atgcggtcgc aatattccgc ttcatccata aagtgggtgg
     6541 tgaccatcac cgtgacgcct ttctctacca tgctgttgat gtgcagccaa aattcacggc
     6601 gggtgagggg gtcaatgccg gaagtcggtt cgtcgagaaa cagaatatcc ggttcatgca
     6661 tcagcgaaca ggccagcgcc agccgctgtt taaaacctaa tggcagttca tcggtggcgt
     6721 gtgaggcaat acttttcagg ccgaacgcct cgctcatacg ggagattttt tcgttctgcg
     6781 cccgaccgcg taagccatac acaccagaga aaaagcgtaa attctgttcg accgtcaggt
     6841 tgccatagag cgaaaacttt tgcgccatat agccgagatg ctggcgcgct ttaccggaac
     6901 tctctttcag atccatcccc agcaccagcg cctgaccgga ggtcggcacc agcaagccgc
     6961 acatcatctt aaaggtggtc gatttccccg cgccgtttgg cccgagcaga ccaaaaatct
     7021 cccctcgttt aacggcaaag ttgacgtgat cggtggcggc aaaatcacca aatttcttgg
     7081 ttagttcttt cgcttcgatc accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg
     7141 cacccagcgg cgattccgag gttccggcac cgcccagcaa atcaataaac gcatcttcaa
     7201 aacgcggcgt agtttcgttg atgttgattt ccggcatccc ttcggcatgg cgaatatcgt
     7261 ctggtgtggc ctcttttttg aggatcagac gtaccgattt cccctgaatc atgccgtcgc
     7321 tgacctgcgg cagtttcaag gcgcgttgca acagtttgcg gttgccctcg tgtggactgg
     7381 tcatcagaaa gctgcgtccg gccatggttt gtgtcagggc ttttggttct ccctgataca
     7441 gcaactcgcc ttcgttcatc agtaacacgt cacggcactg ctcggcttcg tcgagatacg
     7501 aggtactcca gaggattaac atcccttcgc ccgccagctc atgcaccatc tgccacagtt
     7561 cgcgccgtga gatagggtca acgccgacgc cgggttcatc gagcagcaac actttcggtt
     7621 cgcccaccag ggtacaggcc agaccgagtt tttgtttcat cccaccggag agcttgcccg
     7681 ccaggcgtcc ggtaaacggc ccaagagacg taaactccag caggcgagca aaagtttgct
     7741 tacgtgcctc gccggtgacg ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg
     7801 tgagatcttc atacagacca aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc
     7861 cgtcgttttt gattggatca aagccaatca ccgtggcatt gccgctgtcg ggtttcagta
     7921 atcccgccag catccgcatt agcgtggttt tacctgcacc gtccggccct accagccccg
     7981 tcacataacc ggcgtgaatg gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg
     8041 gaaagcgttt ttccaggccg ttcagcgtga taacggcatc attcatgtcc tgcctcgtta
     8101 ccgaattgca ccgtcaccgg cattccctgg cgtaacgcat catcggcgtc ggtcaccaca
     8161 atacgcaggc gatagacgag gtcggtacgc agatccggtg tttcgacggt tttcggggta
     8221 aattcagcag tcggcgaaac gaaaccaatc tgtccgtgat agggcttgtc cgggcgacca
     8281 tcggtataaa gcagcacttt gcgccccggc tgggcctggt caagattccg ttcatcaaca
     8341 taggcacgta cccacaccgg acgcgttagt gaaacggtaa acaccgtgcc accttcattg
     8401 agaaccgtgc ctggctccac cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac
     8461 gttgagtcct gtaaattcag ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg
     8521 gctttcgcct gagcgatgtc ctgttcacgg ttaccggaac ggtactgacg caatttatcc
     8581 tgtgctgatt tcagcgttgc ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca
     8641 tttgccgaaa tggtgcggct tttccacagt ccttgctggc ggttatagaa gttctgcgca
     8701 tagtcatagg cggcttgagc ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg
     8761 cgatacccgg caagcatcag atcatactgc gcctgtgcca ccgagacgcc cgctttcgcc
     8821 tgcatcaggg caatctcata cggcttgtga tccagttcgc ccagcacctg gcccgctttg
     8881 atagcatcac cttcgtccac cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga
     8941 tttaccgtac gaatatccac gttgccatac agcgtcaggc cgttatcctg gcggctttga
     9001 taccaccagt agcctccggc aaccacggcg gcaagtacca ctaccgccaa tccgatcacg
     9061 acaggttttt tcatcactac aaactccttt gcgataatcc ttgcaaaatc aggtcgatat
     9121 gacaggtcac cgtctggttg atcagttcgg tcttttcttc atcgaacgcg gtccagccgg
     9181 tacgtaacag aattgtttct ttgccaagac ggaacgccag aatctcacca atcaacgcat
     9241 gagtatggag gatcatgcgg gtatcgttgg cgtcgcagcc ggtccaggcg gcaatcaggc
     9301 gtgtcaggtg gctgtgtagc gggctaatca cctgttcgtg caccaggtga taggctgccg
     9361 tgggagagag ctgctcacgg gagataaact tgctgaggtt gacggtgtca tcctgggtga
     9421 gcagcttaat catgttcctg caggcgcgaa ggatcagttc acggatggct gcgcgatcag
     9481 gctgtggttg tgcgaacaag cgttcggcct cttcggcgtg cggacggaac tgctcgccaa
     9541 taaaatcggc aatccactgg gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt
     9601 aggtgatggc agcgatattc tgcccggcct gggcggctat ctcgcgggtg gtggcgttca
     9661 ttccatattc accaaactgc gccagtgcgg cagcaatcag ctgttttttc gcctgttcac
     9721 ccttgattgt catggcagga ttattcatcg cacagtccat tcttaatcaa atgattgatt
     9781 aagattatga ctccataggg gagttgtcca gtatggctaa gaattttagc tacgccagtc
     9841 acagggataa tttatgcgct gcgtcacaaa aactgctaca ctccgctccc tcatgacatt
     9901 gtggtttttg tcattttcct tttcagtatc tccctgaaaa ctacaccggt aacggtcggg
     9961 gcggttcgga gtagttatgt ctttcgattc tttgggttta agccctgata tcctgcgcgc
    10021 cgttgccgag cagggttacc gtgaacccac ccctattcag cagcaggcga tccctgcggt
    10081 gctggaaggc cgcgacctga tggctagcgc ccagaccggt accggtaaaa cagcgggctt
    10141 tacgctgccg ctgttgcaac acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc
    10201 ggtacgtgcg ctcattctta ccccgacccg tgaactggct gcgcagattg gcgaaaacgt
    10261 ccgtgattac agcaaatatt tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat
    10321 taacccgcaa atgatgaagc tgcgcggcgg cgttgatgtt ctggtggcaa caccgggacg
    10381 tttgctggac ctggaacatc aaaatgcagt gaagctggat caggttgaaa tcctcgtcct
    10441 cgatgaagct gaccgcatgc tcgacatggg ctttatccac gatatccgtc gcgtgttaac
    10501 aaaactgcca gcgaaacgcc agaacctgct gttctctgcg accttctctg acgatattaa
    10561 agccctggcg gaaaaactgt tgcacaaccc gctggaaatc gaagtggcac gccgcaatac
    10621 cgcgtctgat caggtgaccc agcacgttca ctttgtcgat aagaaacgca aacgcgaatt
    10681 gctgtcgcac atgattggta aagggaactg gcagcaggtg ctggtgttta cccgtaccaa
    10741 acacggcgct aaccatctgg ctgagcagct caataaagat ggcatccgta gtgcggcgat
    10801 ccacgggaat aaatcgcaag gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga
    10861 cattcgcgta ctggtggcga ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc
    10921 gcacgtcgtc aactatgaac tgccaaacgt accggaagat tatgtccacc gtatcgggcg
    10981 taccggtcgt gcggctgcta ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa
    11041 actgctgcgt gatatcgaaa aactgctgaa aaaagagatc ccgcgcattg cgattccggg
    11101 ctatgagccg gacccgtcaa tcaaagccga accgatccag aacggtcgcc agcaacgtgg
    11161 cggcggcggt cgtgggcaag gtggtggtcg cggtcaacag caaccacgcc gtggggaagg
    11221 tggcgcaaaa tctgcaagcg cgaaacctgc agaaaaaccg tctcgccgcc tcggcgatgc
    11281 caaaccggca ggcgaacaac aacgtcgccg ccgtccgcgt aaacctaccg ctgcgcagta
    11341 atcttttatg ccgggctatg cccggcatca ggctgatgaa caaacgcaaa actgcctgat
    11401 gcgctacgct tatcaggcct acgtgaactc tgcaatatat tgaatttgca tgcttttgta
    11461 ggccggataa ggcgtttacg ccgcatccgg catttcacaa caagcacttg tcagcaattt
    11521 gagaacacgg gaaaaaattt atctgtttta ccgcccataa gccaccgaga cgttacttct
    11581 ctatagccaa ttgttcgcgc aactccatta aaaggtagcc cagtcgattc ttgcccttac
    11641 cattaccacc gtctccccag taagcatcgt tttccgtatg ctcaaccagt tttgcgggcg
    11701 cggttgccag caagagcgca cgcagttctg gatgctgttc gaatttagcg cgaagtgctt
    11761 ttcgcatcac ctgttctttg accgactccc agtttttacg cagaggctta gaacgatcgc
    11821 gccccatgcg tgcggcgacc ataggggaag aaacccgacg gatctcttcg cggtattttt
    11881 catcaaggaa tttttgtgcc tgaaaatagt gttctgaggt gggccaggtt ttcccgtcaa
    11941 ccttgatggg ccaggcggca aagttagaaa aatcaccgta gtcatcgctg gtgctgtaga
    12001 aatttatgat ggtgtcttgc atgacgtgtt ggatcctttg tgctcgaacg ggcattaaac
    12061 cgcattatgt tggtggttat tgcgagccgc tttccagaaa cagaaaaacc attacccctg
    12121 aaaaccgaaa aatgccacaa tattggctgt ttatacagta tttcaggttt tctcatggca
    12181 ttaaccgccg cgcttaaagc gcaaattgcc gcctggtata aggcgcttca ggaacagatc
    12241 cccgacttta ttccccgtgc gccgcagcgg cagatgattg cggacgtcgc caaaacgctg
    12301 gccggagaag aagggcgaca tctggcgatt gaagccccca ccggcgttgg gaaaacgctc
    12361 tcttatttga ttcccggcat cgccattgcc cgcgaagagc aaaaaacgct ggtggtgagt
    12421 accgccaacg tggcattgca ggatcagatt tacagcaaag atttaccgct gctgaaaaag
    12481 atcattcccg atcttaaatt cactgccgct tttgggcgtg ggcgctacgt ttgtccgcgt
    12541 aatctgacgg cgctcgccag tactgaaccc acgcaacagg atctgctggc gtttcttgac
    12601 gacgaactga cgccgaacaa tcaggaagag caaaaacgtt gtgcgaagct gaagggcgat
    12661 ctcgatactt ataaatggga tggtctgcgc gatcatacgg atatcgctat aaatgacgat
    12721 ctctggcgtc gtttgagtac cgacaaagcc agctgcctca accgtaactg ttactactat
    12781 cgtgaatgcc cgttttttgt tgctcgtcgg gagattcagg aagcggaagt ggtggtggca
    12841 aaccatgcgc tggtgatggc ggcgatggaa agcgaagcgg tattgcctga cccgaaaaat
    12901 ttactgctgg tgctggacga aggccatcac ctgccggatg tggcgcggga tgcgcttgag
    12961 atgagcgccg aaatcaccgc gccgtggtat cggctacagc tggacttgtt cacgaaactg
    13021 gtcgctacct gcatggagca gtttcgcccg aagaccatcc caccgctggc gatccctgaa
    13081 cgtttgaatg cgcattgtga agagttgtat gagcttatcg cctcgttaaa caacattctc
    13141 aatctctaca tgcctgccgg gcaggaggca gagcaccgtt ttgcgatggg cgaactgcca
    13201 gatgaagtgc tggagatctg ccagcggctg gcaaaactca ccgagatgct gcgtggcctg
    13261 gcggagttat ttcttaacga tttaagtgag aaaaccggca gccatgacat tgtacgtctg
    13321 catcggttga ttttgcagat gaaccgcgcg ttggggatgt tcgaggcgca aagcaaactc
    13381 tggcggctgg cttcgctggc gcaatcttcc ggtgcaccgg tgaccaaatg ggcgacgcgg
    13441 gaagagcgcg aagggcagct acacctctgg tttcactgcg tgggaatacg tgtcagcgat
    13501 cagctggaaa ggctgctgtg gcgcagtatt ccgcacatta ttgtcacctc cgcaaccttg
    13561 cgttcgctga acagtttttc gcgtttgcag gagatgagtg gtctgaaaga gaaagcgggc
    13621 gaccgttttg tggcgctgga ttcccccttt aaccactgcg aacagggcaa aattgttatt
    13681 ccccggatgc gcgttgagcc ttccatcgac aacgaagagc agcatattgc cgaaatggcg
    13741 gcctttttcc gtaagcaggt ggagagcaaa aaacatctcg gtatgttggt actgtttgcc
    13801 agcggacggg cgatgcagcg ctttctcgac tatgtgacgg atttacgtct gatgttgctg
    13861 gttcagggcg atcagccgcg ttaccgttta gttgaactgc accgcaaacg cgtcgccaac
    13921 ggtgagcgca gcgtgctggt gggcttacag tcatttgccg aagggcttga tttgaaaggt
    13981 gatctgctca gccaggtgca tatccacaaa atcgcttttc cgcccatcga cagcccggtg
    14041 gtgatcaccg aaggggaatg gctgaaaagc ctcaaccgct atccgtttga ggtgcaaagc
    14101 ctgccgagcg cctcgtttaa cctgattcag caggttgggc gactgattcg aagccacggt
    14161 tgctggggcg aagtggttat ctacgacaaa cgcttgctga ccaaaaatta tggcaagcga
    14221 ctactggatg cattaccggt atttccgata gagcaaccgg aagtccctga aggtatagtt
    14281 aaaaagaaag aaaaaacgaa atccccacgc cgtcggcggc gttaatgatg tgagtcaggt
    14341 aaggagtcgt aaatggacta tcgcaaaatc attaaagaga tcgggcgcgg gaaaaaccac
    14401 gcgcgcgatt tagaccggga tactgcgcgc ggtctgtatg ctcatatgct caatggtgaa
    14461 gtccctgacc ttgagttggg cggcgtattg attgcgctgc gtatcaaagg agaaggggaa
    14521 gcagagatgc tcggctttta cgaagccatg caaaatcaca ccatcaagct gacgccgcca
    14581 gcaggcaagc cgatgccgat tgtcatcccc agttacaacg gcgcgcgtaa acaggccaac
    14641 ctgacgccgt tgttggcgat tctcctgcac aaactcggtt ttcctgtggt ggttcacggg
    14701 gttagcgaag atccaacccg cgtgctgact gaaaccattt ttgaattgat gggtattacg
    14761 ccaacgttgc acggcgggca ggcgcaggcg aagctcgacg aacatcaacc ggtgtttatg
    14821 ccagtcggcg cgttttgccc gccgctggaa aaacaactgg cgatgcgctg gcggatgggc
    14881 gtgcgtaaca gtgcacatac cctggcgaaa ctggcgacgc catttgccga aggtgaggcg
    14941 ctgcgtcttt ccagcgtttc gcatccggaa tacattggac gcgtcgcgaa gttctttagc
    15001 gatatcggcg ggcgggcgct actaatgcat ggtactgaag gtgaagtgta tgctaatccg
    15061 cagcgctgcc cgcagatcaa tctcattgac cgtgaaggga tgcgggtgct gtatgaaaaa
    15121 caggacactg ctggtagcga gttactgcca caagcaaaag atccggaaac cacggcgcag
    15181 tggattgagc gttgccttgc tggcagcgaa ccgattcccg aatcgctgaa aatccagatg
    15241 gcttgctgcc tggtggctac gggtgaagcg gcaactatca gcgacggcct ggcgcgcgtt
    15301 aatcaggcat tttaattctt ttctcagccg gatgagccat gtcatccggc ttttacccca
    15361 ccgcaatatg aaattcctgc atctttattg accttcccac gcccggcgtg cagcataaaa
    15421 atacaacaaa cacataacat aaacaggagt taaccatgga aagtggtcat cgctttgatg
    15481 ctcagacgct gcacagtttt attcaggctg tatttcgtca gatgggtagc gaggaacaag
    15541 aagcgaaatt agttgccgat catttaatcg cggcaaacct ggcagggcat gattcacatg
    15601 gtattggcat gatcccaagc tatgtacgct cctggagtca ggggcacctg caaattaacc
    15661 atcatgccaa aaccgttaaa gaggcggggg cggcggtcac gctcgatggc gatcgcgcat
    15721 ttggtcaggt cgcggcacat gaagcgatgg cgctggggat tgagaaagcg catcagcacg
    15781 gtattgccgc cgtggcgcta cataactcgc atcatatcgg ccgtatcggt tactgggcgg
    15841 agcagtgtgc agcggcgggg tttgtctcta tccactttgt tagcgtggtc ggtattccaa
    15901 tggtcgcgcc gttccacggt cgcgacagcc gctttggcac caatccgttc tgtgtggttt
    15961 tccctcgtaa agataatttc ccgctgttgc ttgattacgc caccagcgcc attgcatttg
    16021 gcaaaacccg cgtcgcctgg cataaaggcg tccccgtgcc gccaggttgc ctgattgacg
    16081 ttaacggcgt gccgacgacc aatccggcgg taatgcagga gtcgccgttg ggttcgctgt
    16141 tgacctttgc cgaacataaa ggctacgccc ttgcagcgat gtgtgaaatt cttggcgggg
    16201 cgctttccgg cggtaaaacg acgcatcagg aaacgttaca aaccagtccc gatgccattc
    16261 ttaactgcat gaccactatc atcatcaacc cggaactgtt cggcgcgccg gattgtaacg
    16321 cgcagaccga agcctttgcc gagtgggtga aagcctcgcc gcatgatgat gataagccga
    16381 ttttgctacc gggcgagtgg gaagtgaaca cgcgtcgcga acggcagaag caggggattc
    16441 cactggatgc gggaagctgg caggccattt gtgatgcagc gcggcagatt ggtatgccgg
    16501 aagagacgtt gcaggctttc tgtcagcagt tagccagcta aaaaaaagcc cgtccagtgg
    16561 cggacgggca aacaagggta acataggatc aatgagggtt agagcatatg cgtctgtcgg
    16621 caaacagaca gggaaatact tgtgctggac gtagcgtaaa cgcctgatcc gtccaacggt
    16681 tcggattttg taggcctgat aagacgcggt agcgtcgcat caggcatctg ttgccggatg
    16741 cggcgtaaac gccttatcct tcctacgaat tacttataaa ttaccgcagt accgcttaat
    16801 ttattgttgt tggtggcgga agtgatgctg tatccactgg caccggctgc cgcggctttc
    16861 tcagccagtt tcgcttccag ggcatcgagg gtggatgcgc catcggcaga aaccacgccg
    16921 attttattca tgttctgtgc ctgggatgcc gttaccggtt ccgcggcaaa cacgccaaat
    16981 gacagagttg aaagagccat agcagcaaca acagtattga tagttttcat aattaatctc
    17041 tcgcaggtga tttttgtata agtgacgttg tttcgtcgat gtgatgagta tcacgttttt
    17101 ttacgagaga taaaatcgaa gagaattgac cgccttgttc aaataaattg attgatatct
    17161 aacatactga taaaccattt atttgttgta cttatggact tgttttatgc agcgtggcag
    17221 tcacgacagc gttaaaggca cggaatgcca cgcaaaatac aaattatact cgctatacat
    17281 ctcgccagtt gtagttaacg taactggctg tctttcgaac ctctgcgatt atatcctgta
    17341 tacgctggtt tttgtaaatc tttctcctgc tgacaatgaa tgcataagcg cacgccagga
    17401 atggcttccc gacgggcttg cgggatgggg gcaccgcact cttcacattc atccaggctt
    17461 tcgccacgcg gaatttcacc ccgagcgcgg gcaatcgcat cttcaattgt actgttgatc
    17521 tgttcgttga cggcgtcatc gttagcccaa ccggatgcca tatcgtcctc cccatatcaa
    17581 tgcttgtaca gttaagtgta gctaatccag ggacgaactt gggcagttca agcatcagat
    17641 ctccgaccat tcccgcagca gattatgata aagattaagc agcgacagga tctcttcatt
    17701 ttcaccatag cggcttttca gcgactgaat attgttgtcc agttcaaaca gcatggcgcg
    17761 ctttttatca tcacggatca tcgactggat ccacataaat gatgccactc gtacgccgcg
    17821 ggttacgggt gtcacgcaat gcaggctgct ggagggatac aacacgagat cgcctgccgg
    17881 gagttttacc cgatgttgtc cgaaggtgtc attaacgacc agttcgccgc cgtcgtagct
    17941 ttgtggatcg cttaaaaaca gcgtggcaga aaggtcagta cgcatccagc cgttttgtgg
    18001 atggctgcgt actgcgccat ccacatggaa accataggtt tcattgttct gatagcgatt
    18061 aaacagcggc gtggaaaggg tacgcggcaa ggccgcggca aagaataaag catgttggtt
    18121 aaccgcgttc agcacctcat tttgcagggc ggcgtataac gtgctgcggg tgtcgacctg
    18181 ttgattgttc ttaacttgcg caccttgtgc gccggtggtg acgcgtccat ccacccattc
    18241 ggcttgttcc agttgttcgc gaaaccgggc gacgtcctgt ggcgataaca cgccgggaat
    18301 gtggtacatc atacaagttc tccaaaaagt ggggcctgcg ccccacatct gaatcagaaa
    18361 tgcatattgg ctgtgagcaa gaaggttctt ggctcgcccg ggtgataacg gtagccgctc
    18421 ttgttgattg aggcgacgta atcggtatca aacaggttgt aaacgtttag ctggaagtcg
    18481 agattgcgat taacccgata ccccagtttg gcatcggcga cccagtaacc ttcggtaaac
    18541 gctggcgttc ccaccgcgcc gtctgaacct ttatgcatac tgccgatata gcgtgcgccc
    18601 gcgccaacag agatatcatc tgttgcctga tactggctcc ataaggtgaa ggcgtgctcc
    18661 ggggtatacg gcagcgatga ggaaccatcc tgggcaacat ctttgccgtt tttgatggtt
    18721 gctttttgct gggtatagcc gccaatcacc tgccacgcgg gcgtgatatt cccggccacg
    18781 gatatctcat agccttcgac acgtttctta ccgtattgcg agtaagttcc gtcatcattt
    18841 tgctcaactt cattttcgat atcagtacgg aatagcgcgg cggtgagcaa caggcgttta
    18901 tccagaacct gccatttggt gccaatctcg ctggtgttgg ctttttgcgg tttaaaatcg
    18961 gtgcggttgg cactgttacc gctgccagac tgcgcaaggg cgaagttgtt gccgcccgga
    19021 ggctgctggg aaacggcata gttaatatag acattaccgt tttccgtcag gtgatacagc
    19081 gccccggctt tccagttcac cagattgccc gacttggcgg tgtcgacggt ggtgaccgga
    19141 gaaccttttg ccacaccagc tgggcaggtg atggcaccgc gtccgctgcc gccgcaggcg
    19201 gtggcactgt catattcagt atgataatta tccagacgga tcccgccgtt cagctcaaaa
    19261 tcacgggtga tttgcagcgt atcgaaggcg taaattgcga aggtatccgt ctgaccattg
    19321 gcgtttgcgc cgttgcgcgt caggccgccg ggatgaatgc tgctgtcagg atgataaata
    19381 tttaccgcgg gtaacgtcac cggattaacg ccgtagttag tctgcgtttc acgggtaaat
    19441 tccacgccgg tactgacatc atgaccgata gaaccggtat agaacgtcga ggtcaggttg
    19501 gtctggttgg tgagaatttt attactcaca tctttggtat tcgccgtgcg tgaccaggtc
    19561 cagctattca catcgctggt gggctgagta atattcgacg ccccgcccat aatcgccgtc
    19621 atcaggtaat cctgctttac gcgcgaccaa cgggtagtat tgcgaatggt ggtgttatcg
    19681 ttgatgtcgt gctcaaaacg catggtggcg gtgtcggtgg tcgaatcgtc gtaatcggaa
    19741 tccgtgccgt aaaagttatg agtatcaact tttccggaat gattcagagc cgctgttccc
    19801 gcagatgggg cagaataacc cggcaaaccg atggtcggaa tgccgccgtc tggcgtgttg
    19861 tgctgggtga catgcagata attaagatac aaacgattcg ctgtaccaag gccaaaagcg
    19921 atagaagggg cgacgccgta acgctcattt ttgactttgt cgcgtccggc atcgtgcgtt
    19981 ttttcgccca tcacattcag gcgcaccgca gtggtatcac caatgacctg attgacgtcc
    20041 agcgtgccgc ggcggaacca ggcgctgcca atactggcgg aggcgtcaat gccggaatca
    20101 ttgcgcggct gcttgctgat catattgatc gagcccgtcg gcgcgctgcg cccgtagtcg
    20161 gtgccggaag gccctttaat cacttcgacc tgctcagtat tgaaggtgtc gcgcgagacg
    20221 ctgccgatat cgcgaatgcc atcaatataa atactgttag aggtatcagc accgcgcata
    20281 taaatggcgt cgccagtggt ggagttaccg ttctcacccg caaaaaacgc acccacgccg
    20341 gggacgtttt tgagcgcgtc ggtaaggttg gttgcgccct gatctttaat cacttgttca
    20401 gaaatcaccg tcatcgtgcg ggtagtatcc gctaccggac gcgagaattt cggatcggca
    20461 gattgttgtg gcgcataaag cgaaggcgtt gatgcttcga caaccagcgt gtcatccgcg
    20521 ttagtttgcc cttcggcggc gagtgcctga gccacaggcg tgatgccaat acaaagaccg
    20581 gcaaagaacg tgagcgaatg aaattgtctg gcagggaaat tgcgattgtt ttccattttg
    20641 caggtgactt ttttttatat gttgaaattt tcactgccac gagaaagcct ctctgcggat
    20701 gcgatgccac tgttaatctc gtgatagtgt tcgtgcaagt gataataatt ttgataagca
    20761 ttatcacttg gcgcgatttt ctatcaccca ttgcgcgaaa aagaaataca tttatttaca
    20821 ttgcgttcat atttgatgtt ttttgattac aaaacaggcg aaaactgcat ccagaggcga
    20881 atgcagttac gttggtgggg ttatttgtag atggttgcag taccgaacat ctggtcattt
    20941 ccgcctgcgg aattaatgac ataacctttc gcgccttgct cgcgcgcttt ttctgccagt
    21001 ttatcctcca gatcgcttag gtttgatgcg ccagttgccg aaacggtacc ggcgggccgt
    21061 agctgaccgc tgtccaggtt actcatcggt tgcgcggcat aagccgtgag agagattccg
    21121 ctcaggacgg tggcaatcag tagtgtgagg cactttttca taatgacatc ctcataaata
    21181 caacgggtag tgcctgataa gtgtaggtcg cctgggcgat gaaaatgacg caaaaaattg
    21241 acagagatgt gcttattttt tgaacgatat atttttacaa aataagacaa atcaatgaca
    21301 tgcataatgc tgatttttat gatgaattgc cataaaccat agccatgtta aggttttggg
    21361 gaatgataaa cgcagccaga tatttaccct gtcgcgttat gcgggtatct tacgccgctg
    21421 cttaaaggag aatgctatgt ccgcccagaa accggggttg catccgcgca accgtcatca
    21481 cagccgctac gatctcgcca cgctttgtca ggtcaatcct gaactcaggc aattcctcac
    21541 gcttacaccc gcaggggagc aaagcgtaga ctttgccaat ccgctggcgg tgaaggcgct
    21601 caataaggca ttgctggccc atttttacgc cgtagcgaac tgggatatcc cggacggttt
    21661 tctctgcccg ccagtaccgg gccgggcgga ttatattcat caccttgccg atttactggc
    21721 agaagcaagc ggaacaattc cagctaatgc cagcattctg gatatcggcg ttggtgcgaa
    21781 ctgtatttat ccgctgattg gcgtacatga atatggctgg cgttttaccg gtagtgaaac
    21841 cagcagtcag gcgttaagta gtgcgcaggc gatcatcagt gctaatccgg ggcttaaccg
    21901 tgccattcgt ctgcgtcggc aaaaagagag tggggcgatt tttaacggca tcatccataa
    21961 aaacgagcaa tacgacgcga ccttgtgtaa cccgccattc cacgattccg ccgctgcggc
    22021 acgggcaggt agtgagcgta aacgccgtaa cctggggctg aacaaagacg atgcactgaa
    22081 ctttggtggt cagcaacagg agttgtggtg tgaaggtggt gaagtcgcct ttatcaaaaa
    22141 gatgattgaa gagagcaaag gcttcgcgaa gcaggtgatg tggtttacct cgctggtttc
    22201 tcgtggtgaa aacttaccgc cgttgtatcg tgctctgact gacgtgggcg cggtgaaggt
    22261 ggttaaaaaa gagatggccc aggggcaaaa gcagagtcgc tttattgcct ggacctttat
    22321 gaacgacgag cagcgccgcc gttttgttaa tcgccaacgt taaagcgttg gctcccccgg
    22381 cggcagcggt tcagccgggg ccgcgcccgg agcaggcagc acctgataag tctgcactgg
    22441 cgcgcgaaca cctgccaggt cgaaatgctt tttcacctgg ctgtcgaggg caaagcggac
    22501 cgtccactgt ttgagtggca gcgtggtgaa cgaaacacgc agtgtaaacg cggtattgct
    22561 taagccgaca atcccggcaa agtttggttc accaataacc agcccgcgaa tttcttcgtt
    22621 ttccatcaat tccgctaccg catctttcag tgcctgattg gctttatcag catcttcatg
    22681 gcgatcaaca tcgtagtttg ccaccaccga accaatgccg cggacgaagt tagcaaaggt
    22741 ggttatcgaa gaccaaggaa tgatgtgata cgccccggta tcctgacgca cgcccacgga
    22801 gcgaatcgac atccgttcca cagtgccggt caacggcccg atagtaacca aatctccagt
    22861 gttcatgccg ttttcaaact gaataaatac cccggtgata atatctttca ccagcgtttg
    22921 cgaaccaaac gagatagcca gacctaatgc cccagcacct gccagcaatg gcgcgatatt
    22981 gacgccgatt tccgacaaca caatcatgat ggtgatggta ctgataatca ccgccagcgc
    23041 gttacggaac agcgtcagca gggtacgcgt acgggcgctg ggtagcgggc ggccatgaat
    23101 atccgaagcc agccggtttt cgatcaaact ggcgagcact gtccagccaa ccgccgagaa
    23161 gaaaagaatg agtgcgatgc ggatcaggat atctacggtt ttctgccccg cgccgttttg
    23221 cagccagttc cagaaatcga acaatcccca tgcgctcaac agcaacatta ccgccacgca
    23281 gacggtcaga atacgcgccg ttttcagcgc cgccgaaagc cagccattca gccgtttttg
    23341 tagctccgga tagttacgct gagtatgtgg cgagagggtg atggttttcg ccagccagcg
    23401 ggaaaacata ccggaaacaa acgctgcgat accaataatc gccaggctgc gcaccgttgc
    23461 acccatcata aatttcaggc tgttgcccgg atcgaacaac gaaaagaaaa acagcacgat
    23521 aaaataggcg cttgccagcc agtgccacac cagcgcaaag gcgcggataa acaggctgaa
    23581 aaaggccagc gaatgctccg caaagttgag caaatgttgg gtaatctctt ttttattgcg
    23641 aaagatcagg tacaacgccc agacggtcat gcacagcata atgatgacgt tcgccagcgc
    23701 acctatctgt acattcacct gattagagat aatcggcacg gccacaatca ggccataacc
    23761 tatcaaactg cttaaccagc tcaggcgacg actccagtaa cgggcgctct cgtcctgaat
    23821 cgtgaacggg cgcagttcag ccacgtttgg gcaaaaaatc aggcgtagta cggctttgaa
    23881 aaattcaatc agggcaaagg cattgagaaa caaactttgt tggaaagcga tggtgcgact
    23941 gcctgcattc aggttgtcgc ttaatacctg gccgacaaat aatgtcaggg ccagtaacag
    24001 caggtcgata ataaacgccc cgataatcat cgccggaagc tgcaaccagt tgctgcgctc
    24061 acgatttttt tgcctcgccc actgacccat tttgcgatac agcggcagtg cgcacaggcg
    24121 aatcagccag taaaaaccaa acactaatac cgctaacatt gaaaaatggg tcagcgcatt
    24181 gctgaaggtt tgtggattaa atggcttatg cggggagccg gtgatattgc gataaagttg
    24241 cccgaagcgg gcggaaaggg cttcaccata atggcggctg acttcggtga ccttttgcag
    24301 cacggtttgc tcttcaacca gcgtcggcgg tacgatcttc gggaccggtt ccgcaggggg
    24361 cgtcgcggcg acagtacgca actggtcgat caattcttta cgcgaggtgt cgttatccag
    24421 cacatccgcc agtgcgccat aggccgcttt tttttgttcg atatccggtt ccggggccgg
    24481 ttcagtcgtt gagtccgttg tcgttgtggt tgtaacgccg ggtatggata ccgcgtgggc
    24541 aggtgcgccc agcaggcaga agaggatgaa caggatccac cgcatgactc ctccagtgag
    24601 aaaacagagc aaaaagataa gtatagatgc tgggggaggg gcgattttaa atgagaggaa
    24661 tctggtgtgc ctccctttcg ggtgaaaggg aggcagagaa gttaagagac gtgctgcaaa
    24721 aattcctgca agcgctggct cggcgggttc ttgatcaata cctgcggatc gccatcttcc
    24781 gcaatccggc ctttgtcgat aaagatcagc cgcgaagcta ctttctcggc aaaaccgatt
    24841 tcgtgggtca cgatcaccat cgtcatccct tcttcagcca gatcctgcat aaccttcagc
    24901 acttcatggc gcagttccgg gtcaagagcg gaagtcggtt catcaaacag catcattttc
    24961 ggcttcaccg ccagcgcgcg ggcaatcgcc acacgctgct gttgaccacc agaaagttcg
    25021 gaagggtagt gatgtgcacg ttctgccaga ccgactttcg ccagcagctc acgcgccagt
    25081 ttttccgcct cttctttgtt cgcgccacgc acgcgtagcg gaccaaacat gacgttttcc
    25141 agcgccgtca gatgcgggaa gaggtaaaac tgctggaaca ccatacctgc ttcctggcga
    25201 atcaggcgct cgtcaacttt cggatcgtta accttcaggc catcgacaat cagatcgccg
    25261 gaggtgattt cttccagttt gttgatgcag cgcagcaggg tcgatttacc ggaaccggac
    25321 ggcccgataa tcaccacgac ttcgccctgg gcaatgttca aatcgatatt gtgcagcacc
    25381 tgggttgggc caaagtgctt ggagacgttt ttaaattcaa tcacaggatt ttcatccttc
    25441 tttccagacg acgcagaata aagctcagca ccagggtaat aatcagatag aacaccgcca
    25501 cggcgctcca gatctcaagg gcgcggaagt taccggcaat aatttcttgc ccctgacggg
    25561 tcagttccgc cacgccgatc acaataaaca gcgaggtgtc tttaatgctg atgatccact
    25621 ggttacccag cggcggcagc atacgacgca gtgccagcgg taaaatgacg tagcgaatgg
    25681 tttcccaacg tgaaagaccg agcgccagtc ctgcttcacg aaaacctttg tggatagaca
    25741 gcaccgcacc acgcgtgatt tccgcaatgt acgcgccgga gttgatcatg atggtgacca
    25801 ccgccgcagt aaatgggtcg atgcgtaagt cgttaaacgc catcggcagg gcgaaataga
    25861 taaacatcac ctggacgacg ataggtgtgc cgcggatcac ttcaataaag accagcgcga
    25921 cgtggttggc tatccaacca ccgaaggtgc gtgcaaaacc tgccagcaat ccgattacca
    25981 gaccgcctgc cagaccgagg accgaaatcc acagggtcat tttggcacct tcaatcagaa
    26041 gcggaatggc aggccagatg gcactccagt caaactgcat atgttgttcc tgttaccgtg
    26101 gtgtaaataa tcaaattcag ggtaattaat ggccccggag cgggtttgcg cccctcaccc
    26161 taatcctctc cccagagggg agagggaact gccagtgcgt tttacaggtg tagcgttatt
    26221 atttcggttc agtaccgaac cattttttgt agatttcgtt gtaagttccg ttctcgcgca
    26281 gggttttcaa cgcgccgttg actttgtcac gcagctcgtc gctacctttc gggaacgcaa
    26341 taccgtattg ctgcgcttcc agagagtcac ctaccgcttt gaactgaccg ttaccggcgg
    26401 ttttgatgaa gtacagaatg tttggcgtat cgtgcagaac ggcgtctgcg cggttggtgc
    26461 ccagttccat ataggcgtta tcgatgttcg ggaactgacg cagatcttta gttttgatgt
    26521 ttgctttcgc gtaatcaacg gagccagtac cgctcttcac agcaaccact ttcccgtcga
    26581 gatctttcac gcttttcaca tcgttattgt tagctttcac catcactaac aggccgcttt
    26641 tgtagtagcc gtcagagaaa tcgatcgctt ttttacgctc gtcggtgatg gtaatgcccg
    26701 ccagcgccag atcgacgttt ttggtttgca gtgccggaat gatcccactg aaatccatcg
    26761 gcttcagttc gtaatccagc ttcagctctt tagcgatggc cgcccacaga tcaacgtcaa
    26821 agcccacata tttatcgccc tgtttaaatt caaacggaac gaaggcggta tccgtcgcga
    26881 caactaattt tttatccgcg gcatgagaag aaaccgcaaa agccagggtc agtgcagcca
    26941 gtgaaacttt taatacagac ttcatagcat ttcctttttt ctatccacgg gacgatcccc
    27001 tgcgtgacat tcacatatat gaaaaaatcg tgccagtttt gcaactcctt gatttttcga
    27061 gatgcggcaa aaacgtgatg cacgatttat agggcaatac cctgaagatg caccattctg
    27121 gggcaccaat ctggtgcgct aaaattgtgc actcaacaca gtgattattt agcgtaaaac
    27181 ctgatgaaaa aacaatcttt atgtaacgat tgtgtgatga tgtggataca aaaaatttac
    27241 ttaatcagct ggagatagca gatggatgca ctaaataagt gcgttgaggt gggctgcaaa
    27301 acaaaacggc ctcctgtcag gaagccgctt ttatcgggta ctaaagttct gcaccatcag
    27361 cgatggattt attcgatgtt agactcgata aaccacagga atttatccag gtcgcgagac
    27421 gcggcggtca ggatatctgc ggtgtcgtca tctttcgctt cgccaatcgc tttgcgtacg
    27481 tcattagcga cgattgcgta acggtcagcc agttctttca ggtgatcctg aacgttgtgg
    27541 atgtccagcg ggtaactttt cagcggggtt ttgctgttga taacttgagt ggtccccaga
    27601 gctacaccgc ccagctgcac tgcacgttct gccatggtat ccagatgatc gatcagtgcg
    27661 gtgcggaagc catccagcat ttcatgtacg gcaatgaagt tagcgccgcg catgttccag
    27721 tgcgcttgtt tggtaatcaa agaaagatca ataaactgga taacctggcg attcagcaac
    27781 tctactgttg cttttttctc gctgtcggag acatcgttgc gggtataaag cagattggtc
    27841 gcttttgatt taactaattt agcggtactc ataatttcat atcctcttga tgttatgtcc
    27901 cagtaattaa cgagactaag tatagcaccg gctatgtgtt ccgctattct ggctgttcct
    27961 atcacactaa tagtggtaac aagcgtgaaa aacaaaacta ataaagattc aatgagttag
    28021 atatattgat aagaacaatt cttatttatc tgctcgccag gaattttcgg aataaatatg
    28081 cttttgcata attcgatata taaagacggt gtagaggaaa agtagcgaga aattctgcat
    28141 ggttatgcat aaccatgcag aaatgtaaat ttaattaatg tctaattctt ttattttgct
    28201 ctctttgcgt actgtcagcg tagaccccat tgaagcggcg atgatagcgc cgagcgccag
    28261 cagctgtatg ggtgtcagtg tttctccgag gaaaatcatc ccggaaacgg cagccagcgc
    28321 cggttccatg ctcatcagcg taccaaatgt ccgtgttggc aaacgggtga gggcaatcat
    28381 ttccagcgaa taaggcagag cggtcgagag aatagcgaca gccagaccca atggaataac
    28441 cgaccagtgc cagagtgctt caccagcctg aagcgctcca attggcacga aaattaacgc
    28501 tgcaatcaac gaaccaattg ccaccgtcgc agggccatgt tccgctcctg cgcgttgccc
    28561 acttaaaatg taaatagccc aacaagcccc ggcccccagt gccagcgcac agccggttaa
    28621 atcgacatgg gaaacgtctt gccccagcgg tagcaggaac cacagaccaa gaaccgccag
    28681 cacaacccag acgaaatcta ccgggcgacg agaagagaac agcgccaccg ccagtggtcc
    28741 ggtgaactcc agcgccaccg caatacccag cggtactgtc tgaatagaaa gataaaaaag
    28801 ataattcatc ccacccagcg aaacgccgta aaacaacagc ggtaaccgtt gctctttggc
    28861 aaagcgcagt cgccatggct taaagaacgc gatgaggatc agcgttccta atgccagacg
    28921 cagcgcagtg acacccggtg cgcccaccag aggaaaaagt gacttagcta acgaggctcc
    28981 accctgaata gacgccatgg caacgagcaa tatgattatt ggtaaccaga ccggcatttt
    29041 acgtaatgaa ccaggcatcc tttctcccac aaatatctag acttaagtaa agcgtggagt
    29101 gtactggata tacccaatgc tggttgagca tttgttgaaa aaattttccc ccgttttgac
    29161 taaaatgcgc caggattgat ggaatcatta gtctggtgat taggaataat ctggatgaat
    29221 gacagggaaa acatgcgtaa tacttacgca gttctctgaa aaagtgattt aaatttagat
    29281 ggatagcggt gtatggaaac gttctgttac atgaaatggc ccgttagaca tcacaaatcg
    29341 cgaagagttt cccattaatt tttgatatat ttaaaactta ggacttattt gaatcacatt
    29401 tgaggtggtt atgaaaaaaa ttgcatgtct ttcagcactg gccgcagttc tggctttcac
    29461 cgcaggtact tccgtagctg cgacttctac cgtaactggc ggttacgcac agagcgacgc
    29521 tcagggccaa atgaacaaaa tgggcggttt caacctgaaa taccgctatg aagaagacaa
    29581 cagcccgctg ggtgtgatcg gttctttcac ttacaccgag aaaagccgta ctgcaagctc
    29641 tggtgactac aacaaaaacc agtactacgg catcactgct ggtccggctt accgcattaa
    29701 cgactgggca agcatctacg gtgtagtggg tgtgggttat ggtaaattcc agaccactga
    29761 atacccgacc tacaaacacg acaccagcga ctacggtttc tcctacggtg ctggtctgca
    29821 gttcaacccg atggaaaacg ttgctctgga cttctcttac gagcagagcc gtattcgtag
    29881 cgttgacgta ggcacctgga ttgccggtgt tggttaccgc ttctaatcac tttggtgata
    29941 taaaaaatcc gcctctcggg gcggattttt gtttttaagg cttcgggtcg aaaatatcgg
    30001 ttccgagatg gttgtaatcc accttctgta actggaagtt ggtgatgtat atcgacccgg
    30061 ctttcttctc agatataaac gggtatttga tgttaatttc cttcgcttta attcccgtcc
    30121 actgggagaa aaaacctaag aagtcattgg ctgagcggcg ggctttaatc acccgatgcg
    30181 ctttatcgtc gctggaaatg accataaaag gcacctggaa attttgctga tacttatcat
    30241 catgggcaag gtattgcacg tctttgccgc gctctttaaa ggccagaccg tggtcagaaa
    30301 agtaaaccag cgagaagctg ctgccgctgt tgcgtaactg atcgtacagc ttgcgcagta
    30361 aatcgtccgt ttgcgtcatg gtatagagat agcacgacgt ttctttcgac tgcacaaagg
    30421 tttcgtattt gccttgtgtc ctgtcgcagg cctgcggatg tgagcccatc agatgtagaa
    30481 caatcagctg cggttgcgag tgctcttgcg ccagcacttg agcggtcata tccagtaacg
    30541 cttcgtcttt cgtgttttta tctgcttcaa aattaccttc tttcaggaag tacacttcat
    30601 ctgcacgttt ggcgatgctg gcgatagcag tatcgtattc gccgatttgg ccctggttgg
    30661 aaaaccacca ggtctggaag cccgcgcgat ttgccagggt gacaaagtta tcctgaaact
    30721 gtggtttgcc atcgactacg cgattgagcg ttaagccaag cgatttctgc gtggagccac
    30781 tggcggcaat gtagtcagca aatatcaaac cgttaacgct gctggcaaac ggggtattgt
    30841 cccagtgacc gccaaaggca ccgagggcat cgcgacgcgc gctttcaccg atcaccacca
    30901 cataggtctg atacttcggc ttaacggcag tgaccgtcca ggtatctttc attccggaaa
    30961 gttttgccat ccgttcttgc tcttcaatca cctcgttatt attgacgatg acatccttag
    31021 caaaacgaaa taccggatag ccagtatctt tcagtttaaa tacgccaccc caggccaggt
    31081 tttgcacggg agcaacaaaa aatgtcgcca cactgaatac cagacacagg ctgtcgaagg
    31141 tattccagcg cgctttttca ctctcttttt ttcgcctgat tgctattacg ccgagcacaa
    31201 aaataaatat gccgaccatg tagctgtacc acggaaaaat cgtcaggatt tcggtcgact
    31261 cttccatatt ggtggagtgc aatgccagca atgtattgaa gttaggcgcg ccgtaggcct
    31321 gagcaaaagg gaaataacaa gccgccacca gcgaactgat accgaccaga actttttgta
    31381 cgcgaggcaa tgttcgccat agcaaaagca aaatagccgt aaacgcagcg gtgtagagca
    31441 aactgaaggg gtaacctaag ccgaggttaa ttaatagcga ctgtaaaaag tagaacgcag
    31501 tccacgggct aaatacccgg ctacgggtaa caagcgattc tttgagggtt aaattcatat
    31561 gccactatcg taacaaacgc catgtgctta ccctggcgtc aagggtcaat acctgcaaga
    31621 gtgcgtgaag aggggaaagg ggtccgcatc cgcgagccgc aatatacgca gggctgcaag
    31681 aagatagagc gagccgccgt tcaggtcaac tactgacgaa caaatctttt gcgaagggga
    31741 ttgcaaatcc gacaaaaagt ggggtttttc agaccaaaac gcgttatgcc tgcatgaaag
    31801 caggagtgaa gcggcgtacc gcgacgccgc tatttatcgg aaggtttatc ttgctgcggt
    31861 ttgttgttga ccatatcgca caacatagag agcagcatta accgtacttt aaagggagaa
    31921 tgactaaaca cgcgcataca cctcttgaac tcattcataa gacctcctga cttgctaatc
    31981 ccgtcgatcc ttgagggatg attgcattac atacagatat agcacaggct atattatata
    32041 gctattgcta aaacgttaat tttttgtacc ttcgcaactc tggtttacaa tgtgcgcacg
    32101 aaatgagaac gctatgtatg cgtcaccata atgaggaagc acaatgagtc gtcgcgcagg
    32161 tacgccaaca gcaaaaaaag tgacgcagtt agtgaacgtg gaagagcacg ttgaagggtt
    32221 ccgtcaggtc agagaggcgc atcggcgcga gcttattgat gattacgttg agctgatttc
    32281 tgacttgatc agggaagtgg gggaagctcg tcaggtggac atggctgctc gtctgggagt
    32341 ttcgcaaccg acggtggcta aaatgcttaa gcggctggca accatggggc tgattgaaat
    32401 gatcccctgg cgtggcgtgt ttttaacggc agaaggagag aagctggcgc aggaaagccg
    32461 cgagcgacat cagatagtcg aaaatttctt gttggtgttg ggcgtcagtc cggaaatcgc
    32521 ccgtcgcgac gcggaaggca tggagcacca tgttagtgaa gagacgctcg acgcttttcg
    32581 tttgtttacc cagaaacacg gtgccaaatg agcctgcctt ttttacgcac gctgcaaggc
    32641 gatcgttttt ttcagttatt aattcttgtt ggtatcggat taagcttttt cgtgcccttt
    32701 gcaccgaaat cctggcctgc tgctatcgac tggcacacca tcatcacctt aagcggcctg
    32761 atgctgctga ccaaaggtgt ggagttaagc ggttattttg atgtgctggg gcgcaaaatg
    32821 gtgcgccgct ttgctacgga gcgtcggctg gcgatgttta tggtgctggc ggcggcgctg
    32881 ctttctacct ttctgaccaa cgatgtcgcg ctgtttattg ttgttccgct gactatcacg
    32941 ctaaaaagac tgtgtgagat cccggttaat cggctgatta tttttgaggc gctggcagtc
    33001 aacgctggtt cgctactgac gccaattggc aacccgcaaa atattcttat ctggggacgt
    33061 tctggtcttt cgtttgccgg atttattgcc caaatggcac cgctggctgg cgcaatgatg
    33121 ctgacgctcc tgctcctgtg ctggtgttgt ttccctggaa aggcgctgca ataccatacg
    33181 ggggtgcaaa caccggagtg gaaaccgcgg ctggtgtgga gttgtctggg gctgtatatc
    33241 gtctttctga cggcgctgga gttcaaacaa gagctgtggg gactggtgat tgtggcggca
    33301 ggctttgcgc tgctggcacg tcgcgtggtg ctcagtgtgg actggacgct gctgctggtg
    33361 tttatggcga tgtttatcga cgtccattta ctgacccagc ttccagcgtt gcaaggcgtg
    33421 ttgggtaacg tgagtcatct atctgaaccc gggttatggt taacggcaat cggtttatcg
    33481 caggtgatca gtaacgtgcc gagtaccata ttgttgctga actatgtgcc gccgtcttta
    33541 ttactggtat gggcggtaaa cgtaggtggc tttgggttat tacccggatc gctggcaaat
    33601 ttgattgcgc tacgtatggc gaacgatcgc cgcatctggt ggcgtttcca tctctattca
    33661 ataccgatgc tgttgtgggc ggcgttggtg ggatatgttt tgttagttat actcccggcc
    33721 aactagggtc tggcaaataa aaaaggcgga ttatgagtcc gccttttgct ttaagttttg
    33781 aagattaatt cagacgaacc ggcatcccgg agcggttttt aatcgcttca tcaagaacaa
    33841 cctggtcaac atctggctga ccggtcactg tttgcacgct cttcgtcagg gtaattggca
    33901 caatttcctg accttcaaac tgggcttcgg tggtagacag cgggttatgg acttcaatat
    33961 aacggctgcc gtctggctcg gtggtcgctt ttaccggctc atcaataaac tgtacgcggg
    34021 taccgaccgg tactttctcg aacaggaatt tgatgtcttc gttacgcaga cgcacacaac
    34081 catgacttac acgcaggccg ataccgaagt tggcgttggt gccatggata gcatacaggc
    34141 gaccgatgta gagtgcatac agacccatcg ggttatccgg acctgccgga acgacagccg
    34201 gaagcggttc gcccgcagcg cggtactctg cgtgcatttt ggcggtcggc gtccaggtcg
    34261 ggcctgcttt cttacgctca actttggtgg tccagttgat aggcgtatct ttgcctaact
    34321 gaccaatgcc gatcggcagc acgataacgg tgttggtccc tttcggatag taatacagac
    34381 gcatctctgc actgttaatg acgatgcctt catgaacggt atccggcagg atcagctgct
    34441 gcggaatgtt cagtacagta ccgcctttcg gcaggaaggt atccacaccc gggttcgctt
    34501 ccatcatatt ggaaagcccc atctggtact ccgcggcaaa atactccagc ggctgagtgt
    34561 taccttcagg aatggtgatc acctgattct gaccaaccag gcgactcccg tcggttggca
    34621 gaggataagt taccgcagag gcggtactgc aaaagccgac aacagcgaag gccgctgcga
    34681 ataatgtttt caatttcata ttcatgttaa gcgagatttt gtgcctggca ggccattggg
    34741 ttgagaatat tagagtattg gaagcgcatt ataagttcat tccagctcac agtgaaatca
    34801 gatgtgtacg aaatcacatt ttttgccttt ggcttgagtg tagaccttaa gcgaggagca
    34861 ggatcttctt tcagacttat ggcataatgc gcggtttgtc atatctcttt tcaggatacg
    34921 cctgtgttag tttccagtaa cgtcaccatg cagttcggca gtaagccgtt gtttgaaaac
    34981 atttccgtca aatttggcgg cggcaaccgt tacggcctga ttggcgcgaa cggtagtggt
    35041 aaatccacct ttatgaagat cctcggcggc gacttagagc cgactttggg taacgtttcc
    35101 ctcgatccca acgagcgcat tggtaagctg cgtcaggatc agtttgcctt tgaagagttc
    35161 actgtgctgg atacggtgat catggggcat aaagagttgt gggaagtgaa gcaggagcgt
    35221 gaccgcatct atgctttgcc ggaaatgagt gaggaagacg gctataaagt ggccgatctg
    35281 gaagttaaat acggcgaaat ggacggttac tctgcggaag ctcgcgccgg tgaattgttg
    35341 cttggcgtgg gaattccagt ggaacagcac tacggcccga tgagtgaagt tgctcctggc
    35401 tggaagctgc gtgtgcttct ggcgcaggcg ctgtttgctg atccggatat tctcctgctc
    35461 gacgaaccga ccaacaacct cgacatcgac accattcgct ggctggaaca ggtgctgaac
    35521 gagcgtgaca gcaccatgat catcatctcg cacgaccgtc acttccttaa catggtctgt
    35581 acccacatgg cggatctgga ttacggcgag ctgcgcgttt atccgggtaa ctacgatgag
    35641 tacatgacgg cggcgaccca ggcgcgtgaa cgtctgctgg ccgataacgc caagaagaaa
    35701 gcgcagattg ctgagttgca atctttcgtt agccgcttta gcgccaacgc ctcgaaatct
    35761 cgccaggcaa cttcgcgcgc gcgccagatt gataaaatca aactggaaga ggtgaaagcc
    35821 tccagccgtc agaacccgtt catccgtttt gaacaggata agaaactgtt ccgtaacgcg
    35881 ctggaagtgg aaggtctgac caaagggttt gataacggtc cactgtttaa aaatctcaac
    35941 ctgctgctgg aggtcggtga aaaacttgcg gtactgggta ccaacggcgt cggtaaatca
    36001 acgctgctga aaacgctggt gggcgatctg caaccggaca gcggcaccgt aaaatggtct
    36061 gaaaacgcgc gcattggtta ctacgcccag gaccacgaat atgagtttga aaatgatctg
    36121 accgtgttcg aatggatgag tcagtggaag caggaaggcg atgacgagca ggcggtacgc
    36181 agcattctcg gtcgtttgct gttcagccag gacgacatca aaaagccagc taaagtgctt
    36241 tccggtgggg aaaaagggcg gatgctgttt ggtaagttaa tgatgcagaa gccgaacatt
    36301 ctgatcatgg acgaaccgac caaccacctg gatatggaat ccatcgagtc actgaacatg
    36361 gcgctggaac tgtatcaggg cacgctgatc tttgtttcac acgaccgtga gttcgtaagc
    36421 tccctggcga cccgcattct ggaaatcacc ccggaacgcg tgatcgactt tagcggtaat
    36481 tacgaagatt acctgcgtag taaagggatc gagtaagtga ttatccgtca ccccgatact
    36541 gtcggggtga cggcggctga tgacaaatgc aaaat
//
LOCUS       DAZGVQ010000032        36209 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000032 DAZGVQ010000000
VERSION     DAZGVQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.032"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            275..1807
                     /gene="pntA"
                     /locus_tag="ACHLAG_001551"
     CDS             275..1807
                     /gene="pntA"
                     /locus_tag="ACHLAG_001551"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFP0654805.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            1818..3206
                     /gene="pntB"
                     /locus_tag="ACHLAG_001552"
     CDS             1818..3206
                     /gene="pntB"
                     /locus_tag="ACHLAG_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFP0654806.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTQMLFGD
                     AKASVDAILKAL"
     gene            complement(3231..4265)
                     /gene="tqsA"
                     /locus_tag="ACHLAG_001553"
     CDS             complement(3231..4265)
                     /gene="tqsA"
                     /locus_tag="ACHLAG_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFP0654807.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            4677..5042
                     /gene="mdtJ"
                     /locus_tag="ACHLAG_001554"
     CDS             4677..5042
                     /gene="mdtJ"
                     /locus_tag="ACHLAG_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFP0654808.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            5029..5358
                     /gene="mdtI"
                     /locus_tag="ACHLAG_001555"
     CDS             5029..5358
                     /gene="mdtI"
                     /locus_tag="ACHLAG_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFP0654809.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(5397..6218)
                     /gene="ydgD"
                     /locus_tag="ACHLAG_001556"
     CDS             complement(5397..6218)
                     /gene="ydgD"
                     /locus_tag="ACHLAG_001556"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFP0654810.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(6318..6401)
                     /locus_tag="ACHLAG_001557"
     CDS             complement(6318..6401)
                     /locus_tag="ACHLAG_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654811.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(6494..6802)
                     /gene="asr"
                     /locus_tag="ACHLAG_001558"
     CDS             complement(6494..6802)
                     /gene="asr"
                     /locus_tag="ACHLAG_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFP0654812.1"
                     /translation="MKKVLGLVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            7073..7177
                     /locus_tag="ACHLAG_001559"
     CDS             7073..7177
                     /locus_tag="ACHLAG_001559"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFP0654813.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(7226..8479)
                     /gene="ynfM"
                     /locus_tag="ACHLAG_001560"
     CDS             complement(7226..8479)
                     /gene="ynfM"
                     /locus_tag="ACHLAG_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0654814.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            8586..9479
                     /gene="ynfL"
                     /locus_tag="ACHLAG_001561"
     CDS             8586..9479
                     /gene="ynfL"
                     /locus_tag="ACHLAG_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0654815.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            9614..10834
                     /gene="mlc"
                     /locus_tag="ACHLAG_001562"
                     /gene_synonym="dgsA"
     CDS             9614..10834
                     /gene="mlc"
                     /locus_tag="ACHLAG_001562"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFP0654816.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            10959..11654
                     /gene="bioD"
                     /locus_tag="ACHLAG_001563"
     CDS             10959..11654
                     /gene="bioD"
                     /locus_tag="ACHLAG_001563"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP0654817.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(11607..12899)
                     /gene="clcB"
                     /locus_tag="ACHLAG_001564"
     CDS             complement(11607..12899)
                     /gene="clcB"
                     /locus_tag="ACHLAG_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFP0654818.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVGLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(13058..13672)
                     /gene="dmsD"
                     /locus_tag="ACHLAG_001565"
     CDS             complement(13058..13672)
                     /gene="dmsD"
                     /locus_tag="ACHLAG_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFP0654819.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(13715..14569)
                     /gene="ynfH"
                     /locus_tag="ACHLAG_001566"
     CDS             complement(13715..14569)
                     /gene="ynfH"
                     /locus_tag="ACHLAG_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFP0654820.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(14571..15125)
                     /locus_tag="ACHLAG_001567"
     CDS             complement(14571..15125)
                     /locus_tag="ACHLAG_001567"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex B
                     subunit"
                     /protein_id="HFP0654821.1"
                     /translation="MVSNNFKDLGPEVSFRRIYEYAGGDWQEDNGVWHQNVFAYYLSI
                     SCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGCRYCHMACPYGAPQYNAEKGHM
                     TKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQKHGTLAAVAPLPRAHFTKPNI
                     VIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(15163..16326)
                     /locus_tag="ACHLAG_001568"
     CDS             complement(15163..16326)
                     /locus_tag="ACHLAG_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0654822.1"
                     /translation="MSLFRRGEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVDRLGDMPDVTFEEACLRWLEEKADKKSIDSDKSRIAFWIEHFEGIRIKDISEAM
                     IYSVISKAYNRKTKERWKLQVEAALRKGKEPPAYIPKSVSTQTKATHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVKNKRVRWLEKEEARRLIDACSDPLKSVVKFALATGLRRSN
                     IINLEWQQIDMQRRVAWVNPEDSKSNRAIGVALNDTACKVLRDQIGKHHRWVFVYTTA
                     ARRPDGTMTPSIRKMRLDYNTSWLTACRRAGIENFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDILSDDVPNLSHPEVFEDAKKA"
     gene            complement(16458..16553)
                     /locus_tag="ACHLAG_001569"
                     /pseudo
     CDS             complement(16458..16553)
                     /locus_tag="ACHLAG_001569"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(16588..16839)
                     /locus_tag="ACHLAG_001570"
     CDS             complement(16588..16839)
                     /locus_tag="ACHLAG_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0654823.1"
                     /translation="MFVLIQRGQSFVDANNYPVEICKVTLTQVIYRRLDGRTRATSIG
                     AFNEEFERVDHNELHMIKAEIEKEMHIASLRKMRRTSIN"
     gene            complement(16887..17567)
                     /locus_tag="ACHLAG_001571"
     CDS             complement(16887..17567)
                     /locus_tag="ACHLAG_001571"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP0654824.1"
                     /translation="MTPEIILQRTGIDVRGVEQGDYAWQKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARALFEFTSGVNITE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQF
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMAGFDEMVPEFIEKMDEALAEIS
                     FVFGEQWR"
     gene            complement(17564..18349)
                     /gene="bet"
                     /locus_tag="ACHLAG_001572"
     CDS             complement(17564..18349)
                     /gene="bet"
                     /locus_tag="ACHLAG_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFP0654825.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQNDE
                     SCTCRIYRKDRNHPICVTEWMDECRRAPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTTERQPERDITPVNEETMSEINALLTSMEKTWDD
                     DLLPLCSQIFRRYIRASSELSQAEAEKVLGFLKQKATEQKVAA"
     gene            complement(18355..18651)
                     /locus_tag="ACHLAG_001573"
     CDS             complement(18355..18651)
                     /locus_tag="ACHLAG_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP0654826.1"
                     /translation="MNAWLIPDRIEEQSWARHYRQITREENETELADDLEKGLPQHLF
                     ESLCIDNLQRHGASKKAIARAFDDDVDFQERMAEHIRYMVETIARHQIYIDSEV"
     gene            complement(18648..20693)
                     /locus_tag="ACHLAG_001574"
     CDS             complement(18648..20693)
                     /locus_tag="ACHLAG_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFP0654827.1"
                     /translation="MSADKQTFALHCEAKNDKVRKRLGIKGGFFWADTRKLSVAVSRC
                     IAAMDDAGYDEDDFKKPVRVNFPVVNDLPPEGVFDTEFCNRYEKGGEDGITMMAIPFD
                     DNINGEDATTAGDDYDNLDGTIPDDVEKSESPDSDDDCSECEIPVATLSLTHRFLHLF
                     FSKDEDGKYRHHATPEQRNNVIRMEMDTEDSYLQSLLTAVRSHPELDKLTNYHLGRLA
                     QSVGKAFSHATPRHISPAEFDKFISTWMKTDYVDQGLLAKEWQKGNYVTGITRTPSGA
                     NAGGGNLTDRGEGFTHNQASLARDIATGVLARSMDVDIYNLHPAHAKRVEEIVAENKP
                     PFSVFRDKFIAMPGGLDYSRAIVVASVKEAPIGIEAIPARVTEYLNKVLTETDHSNPD
                     PEIVEIACGRSSAPMPQRGTAEEIHDDEEKQQTSDAMSNEQAAPESVEEIPVKHNADT
                     QSLENVSSVETKYQELRAELYEARKNILPKNPVDADKLLAASRGEFVEGISDPTDPKW
                     VKGIQTRDTEDQNQSKVEQNSPDTQQNGPEEQQPGPVMQQEVEKVCTACGQNSGGHCP
                     DCGPVMGDETYAETFGENETAAGDDQAQMKNEYIQKNAVDTTQEHDSVVHIDPDSDAS
                     DNNDDSEQADFDWKRQLVLAAVYGLCANPACVATAPAIPDIAIMIANRLENFGGDKS"
     gene            complement(20802..21188)
                     /locus_tag="ACHLAG_001575"
     CDS             complement(20802..21188)
                     /locus_tag="ACHLAG_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654828.1"
                     /translation="MKELAQNEFFSETSPDAINELKEIAERISKICKKHKIDFVFSFS
                     VLTEVGNNEYKDSRFVSFGLYCETTSPYIHAASEIVDSHIDPQRIQELTLALQLLREN
                     GECNCSDCQHEKKKTTHKTTNQATLH"
     gene            complement(21279..21494)
                     /gene="kil"
                     /locus_tag="ACHLAG_001576"
     CDS             complement(21279..21494)
                     /gene="kil"
                     /locus_tag="ACHLAG_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560215.1"
                     /note="Rac prophage Kil protein; Kil overexpression leads
                     to morphological defects and inhibits cell division;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP0654829.1"
                     /translation="MIAHHFGTDEIPRQCVKPGDYVLHEGRTYIASANNIEKRKLYIR
                     SLTTKTCITDCMIKVFIGRDGLPVKAE"
     gene            complement(21826..22182)
                     /locus_tag="ACHLAG_001577"
     CDS             complement(21826..22182)
                     /locus_tag="ACHLAG_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654830.1"
                     /translation="MNETELKHIIALLLEDARQVYRLSPNSATQERIRMAEKALKQES
                     ENNISEVSANGENEAIEINSNISNGVIASSHKTILMGARLLEVITSELEKNGIKPTPC
                     YLECVLNVIAYSMFRR"
     gene            complement(22214..22927)
                     /locus_tag="ACHLAG_001578"
     CDS             complement(22214..22927)
                     /locus_tag="ACHLAG_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654831.1"
                     /translation="MTTPSVLPQKLWRPLAEIKNFVEKMPDGVRLTEVTKKVKTFAEL
                     SGKERNQLIDFIDKRESIIVFKVRKEGSGNGVTFFRHKKYGYPKREGNVTIIKDLQSK
                     LCTRCGQTKSVDDFYSDASKRDGRAIYCKKCESAMKRSRRECNKLILQQQEPEMNNLK
                     AVSPSPEILRKQAEELLKAAEIAEKKRQEDDVFNKKLAPLKLEILQAAGKMQLKLDEF
                     IDCMDEMNKAVQKLKELTA"
     gene            complement(22982..23053)
                     /locus_tag="ACHLAG_001579"
     CDS             complement(22982..23053)
                     /locus_tag="ACHLAG_001579"
                     /inference="COORDINATES: protein motif:HMM:NF022510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction Ral family protein"
                     /protein_id="HFP0654832.1"
                     /translation="MVWAIRTTAMIARELSKQKNKAA"
     gene            complement(23281..23619)
                     /locus_tag="ACHLAG_001580"
     CDS             complement(23281..23619)
                     /locus_tag="ACHLAG_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0654833.1"
                     /translation="MLMVKKTIYVNPNRGQNRKVSDRSLTSRDRRRIARWEKRIAYAL
                     ENGITPGFNAIDDGPEYKINEDPMDKVDKALATPFPRDVEKIKDEKYEDIMHRVVNHA
                     HQRNPNKKWS"
     gene            complement(23588..23926)
                     /locus_tag="ACHLAG_001581"
     CDS             complement(23588..23926)
                     /locus_tag="ACHLAG_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFP0654834.1"
                     /translation="MVTIIWKESKGTAKSRYKARREELIAERRSSERLTRKIALKISG
                     CVRADKAASLGSLRNKKAEEVERKNRSTYYKDSNPLGNKIHAVQKIKLYSKPPYGLIE
                     YAYGEKDYLC"
     gene            24272..24481
                     /locus_tag="ACHLAG_001582"
     CDS             24272..24481
                     /locus_tag="ACHLAG_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654835.1"
                     /translation="MLIEGEFGVLHQRVVEILGVSLLEVIATGEAISADAIAGMIRVL
                     HHDELDDLAVKLAIDVLLQDMRLCN"
     gene            complement(24634..25341)
                     /locus_tag="ACHLAG_001583"
     CDS             complement(24634..25341)
                     /locus_tag="ACHLAG_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP0654836.1"
                     /translation="MKTFAERLNAAMSSAGLSQAQLADMVGVSQPAIQKMSSGKTTGS
                     RKMVELANALRVRPEWLSSGIGSMRPQSAEEPSNARESSLKAIAWDDHQNDNDEFVAL
                     PLLNISLSAGGGSCALEESAEFSLVFRRYYLKKMGVPEKAAKLVRVVGQSMEPTLHDG
                     DVVGVNTQDTSIRDGKTYAICQADLLRVKTLIATPTSVIIRSINREEYPDEVMNREEF
                     YKNVKIIGRVFWSSHSW"
     gene            25452..25676
                     /locus_tag="ACHLAG_001584"
     CDS             25452..25676
                     /locus_tag="ACHLAG_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0654837.1"
                     /translation="MNLVIQRALNIVGSQKRLAADCGVSQPAVHKWLRGGKVSPEKVF
                     AIVNATNGQVKAYEIRPDLPHLFPHPNQAE"
     gene            25792..26088
                     /locus_tag="ACHLAG_001585"
     CDS             25792..26088
                     /locus_tag="ACHLAG_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFP0654838.1"
                     /translation="MSQTSYSKLSQRDIDRAETDLLINLSVLTQRGLAKMIGCHESKV
                     SRTDWRYIAAILCAFQMASDISPISRAFQHAIRVHANKKRPVGAGRSEQILMNI"
     gene            26103..26231
                     /locus_tag="ACHLAG_001586"
     CDS             26103..26231
                     /locus_tag="ACHLAG_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654839.1"
                     /translation="MEQYTGIILPHLEEFRQQQHQPQQPEADWVNPGDTGTVCGME"
     gene            26252..27334
                     /locus_tag="ACHLAG_001587"
     CDS             26252..27334
                     /locus_tag="ACHLAG_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0654840.1"
                     /translation="MRNYATISPQFWLGDTGRKLRKSGPECMVVALYMMTSPHSNMLG
                     LYYLPVLYIAHETGLDPEGASKGLQMACEAGFCSYDHDSEVVWVHEMAAWQVGEALKP
                     GDNRCAGVRNEYSALLENPFLSSFYDRYKDDFHLDVRRESCRKIEAPSEPLSSQEQEQ
                     EQEQERDKTLLVHGEKIATDPLVDSCPVLTERPGPAGMTPEADSGRCVQQVLHVEPEQ
                     QHQPQQPEADSAMSGKPIGLTRAMPGPSAGRVDYPDVFERVWREYPHRAGSNPKKSAF
                     NAWRARLREGVSPDVVLDGVRRYARYLEATGKAGTEFVQQASTFFGPNRNFENPWSLP
                     KAGAVSLRCVNHISEPDTEIPPGFRG"
     gene            27341..28077
                     /locus_tag="ACHLAG_001588"
                     /pseudo
     CDS             27341..28077
                     /locus_tag="ACHLAG_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789014.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            28103..28873
                     /locus_tag="ACHLAG_001589"
     CDS             28103..28873
                     /locus_tag="ACHLAG_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP0654841.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGESGVTEGQALQSEAPDVVTEEVAPKVSADMMIEFIAQEGAKTCEEI
                     AGKFGVTTRKVASTLAVVTATGRLVRVSIKGKFRYKVPDVENGDLSASSSQDENITHE
                     AENSASGDELNRFPLMSEGTAKACTDEFIRDVPSFTEKYAGAVTLPPLRAASRELRRA
                     KKQVQKWERVCAALRELNKHRELVMQITDSSSSIASEK"
     gene            28888..29319
                     /locus_tag="ACHLAG_001590"
     CDS             28888..29319
                     /locus_tag="ACHLAG_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP0654842.1"
                     /translation="MAKVFTPEQREELKARIVELVRLNGRGTFRQLATETGVSKTAVR
                     RLSRELVVSGAIFDSGYGLFPSDQARKDWQNARKKLSRAKAKKPVVIDPGLIRALPDG
                     EIRRYDRRYNIICRECRNSETMQRVLAFWRGSAEGLMFSPS"
     gene            complement(29352..30026)
                     /locus_tag="ACHLAG_001591"
     CDS             complement(29352..30026)
                     /locus_tag="ACHLAG_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HFP0654843.1"
                     /translation="MSLVSFDITCPHCLKERAVIEAFAEKQIEKTPLFNVAFVCRSCH
                     RGGVAIVEIPSDHYHGPMAESQKKDLDILISGNSQYRFRKIYPAVKKITAPEYTPPVA
                     DRTFVEAKEDLQRRRYDTVVILCRRVLDISTKKLLGDEAGKESLSQRIQMIYKKGLIT
                     EQMKEWAHIVRIDANKAVHTDEVFTPIEASQILSFTEMFLVYAFTLPAMVAARREQKR
                     SDSASI"
     gene            30025..30270
                     /locus_tag="ACHLAG_001592"
     CDS             30025..30270
                     /locus_tag="ACHLAG_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654844.1"
                     /translation="MDIPLEIKEINEIIEVTDRPEFTLMRRYETGTDEQKYIMVAALA
                     VMAIERERREKDVMVIPARNDSPDLRWQDPEWDVAIR"
     gene            complement(30318..30623)
                     /locus_tag="ACHLAG_001593"
     CDS             complement(30318..30623)
                     /locus_tag="ACHLAG_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP0654845.1"
                     /translation="MAMFNPPHPGEIIADILEDQNIGIRELARALDVAPSTVQRLVSG
                     NATISPEMAVRLAAVLGGTPSSWIRLQTAWSLEKAEREVDVSHLSTKYRPAEISPHA"
     gene            complement(30623..30901)
                     /locus_tag="ACHLAG_001594"
     CDS             complement(30623..30901)
                     /locus_tag="ACHLAG_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005188495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0654846.1"
                     /translation="MIKSWKHKGLQRFFETGNASGVNPNHVKRLRTRLQVIDQAEIID
                     DINLAGYRLHQLQGDRSDTWSVTVSGNWRITFEFRNGDAYILNYEDYH"
     gene            31236..31547
                     /locus_tag="ACHLAG_001595"
                     /pseudo
     CDS             31236..31547
                     /locus_tag="ACHLAG_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232082.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            31816..32478
                     /locus_tag="ACHLAG_001596"
     CDS             31816..32478
                     /locus_tag="ACHLAG_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFP0654847.1"
                     /translation="MTMQLAVESLVVVTYSNVPVITTGLLANLYKTEEKHIRQNFKRN
                     ECRFIAGKHFFKVSGCELDSLRTSQRGLQISPKTRSLILWTERGAARHAKMLETDQAW
                     EVFEKLEDCYFSQGKTTQTEQQSQIQPQFTAEEIILLCYMQLWMEKAQDLSKHLYPIM
                     KELNSSYTNKLYDITFETIYMVTKNRDALLREAARLDQSSFVVQRARPMLKSLRARQF
                     EF"
     gene            32733..33377
                     /locus_tag="ACHLAG_001597"
     CDS             32733..33377
                     /locus_tag="ACHLAG_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HFP0654848.1"
                     /translation="MSTQLVFKSHVLETIEHDGKQWFTAATLASALEYSRGDKITQIY
                     NRNSDEFTPCMSINLKMRFNGINNSLREKDVRIFSLRGAHLVAMFATTPVAKEFRRWV
                     LDILDREAEKSAIPPQPHIQPQFTAEDIILLCYVQLWMEKAQQLSKQLYPVMRELNSD
                     YYGKLFDLAYETRHITNRTRDTLLREAAKLDPANYMVHRARSMLAQLRARQFEF"
     gene            33432..33842
                     /locus_tag="ACHLAG_001598"
     CDS             33432..33842
                     /locus_tag="ACHLAG_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFP0654849.1"
                     /translation="MNQTIFLRSKQQQQFAINAILATTLDKDKPVTIRITDYKRNLDQ
                     NAKFHAMVADISRQVQWCGRWLKPEQWKVLLISGHAVATKQEADVLPGLEGECVNIRE
                     SSAQMSVKRMASLIEYTTSWAVEKGVRFTDRRYE"
     gene            33839..34030
                     /locus_tag="ACHLAG_001599"
     CDS             33839..34030
                     /locus_tag="ACHLAG_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP0654850.1"
                     /translation="MRRQRRSITDIVCENCNYLPTKRSRNKPRPIPNESDVKTFNYTS
                     HLWDVRWLRRRARFNSHSG"
     gene            34054..34344
                     /locus_tag="ACHLAG_001600"
     CDS             34054..34344
                     /locus_tag="ACHLAG_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFP0654851.1"
                     /translation="MANLRKEARGRECQVRIYGVCNGNPETTVLAHYRIAGICGTGMK
                     PDDLLAAWACSDCHNEIDRRTRILDNNDARLYHLEGVIRTQAILLKEGKIKS"
     gene            34341..34703
                     /locus_tag="ACHLAG_001601"
     CDS             34341..34703
                     /locus_tag="ACHLAG_001601"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0654852.1"
                     /translation="MNEYEFVLPWPPTVNTYWRRRGSQYYISDKGQKYRKDVQQIIRQ
                     LRLDIFTKSRLRITIIAEPPDSRRRDLDNILKGLLDSLIYAGFAEDDEQFDDIRVIRG
                     VKVPGGRVGIKITELENI"
     gene            34703..34897
                     /locus_tag="ACHLAG_001602"
     CDS             34703..34897
                     /locus_tag="ACHLAG_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFP0654853.1"
                     /translation="MNAKIQTIPELLICTRGNQTEVARILNCNRATVRKYIDDKDAKK
                     HAVVNGVLMVHRGWGKDTDA"
     gene            34890..35270
                     /locus_tag="ACHLAG_001603"
     CDS             34890..35270
                     /locus_tag="ACHLAG_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP0654854.1"
                     /translation="MRDIRQVLERWGAWAANNYEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDGTIGKKLQKA
                     EGVVEGMLMLLGVRLEMDRYVQRL"
     gene            35403..35996
                     /locus_tag="ACHLAG_001604"
     CDS             35403..35996
                     /locus_tag="ACHLAG_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654855.1"
                     /translation="MDIEDFLEAEDNDLSLWNDDILSIDWFVILRELLKYQRRLNITH
                     SELVLLANLVSFHQNVDSQILPSISLLATRMRASRAIIQGRLQELEGKGMLQKISYAQ
                     IGHDDDLRNVYDIKPLIRKLIGVQKTENDKKHTCPVCGKVGSSDEEIEKFFGFRKSGD
                     YKWPQSWCRSCRSTKQRRLNTSLIGKKRPRKSPPEVV"
BASE COUNT         9115 a         8612 c         9064 g         9418 t
ORIGIN      
        1 tctcgtttta cccgaccgtc gaagacaatt atcagtcttt atccggcgtt ctaaggtgtt
       61 tatcccacta tcacggctga atcgttaata ttttgcgagt tcacgccgaa atactgattt
      121 ttggcgctag atcacaggca taattttcag tacgttatag ggcgtttgtt actaatttat
      181 tttaacggag taacatttag ctcgtacatg agcggcttgt gtggctcctg acacaggcaa
      241 accatcatca ataaaaccga tggaagggaa tatcatgcga attggcatac caagagaacg
      301 gttaaccaat gaaacccgtg ttgcagcaac gccaaaaaca gtggaacagc tgctgaaact
      361 gggttttacc gtcgcggtag agagcggcgc gggtcaactg gcaagttttg acgataaagc
      421 gtttgtgcaa gcgggcgctg aaattgtaga agggaatagc gtctggcagt cagagatcat
      481 tctgaaggtc aatgcgccgt tagatgatga aattgcgtta ctgaatcctg ggacaacgct
      541 ggtgagtttt atctggcctg cgcagaatcc ggaattaatg caaaaacttg cggaacgtaa
      601 cgtgaccgtg atggcgatgg attctgtgcc gcgtatctca cgcgcacaat cgctggacgc
      661 actaagctcg atggcgaaca tcgccggtta tcgcgccatt gttgaagcgg cacatgaatt
      721 tgggcgcttc tttaccgggc aaattactgc ggccgggaaa gtgccaccgg caaaagtgat
      781 ggtgattggt gcgggtgttg caggtctggc cgccattggc gcagcaaaca gtctcggcgc
      841 gattgtgcgt gcattcgaca cccgcccgga agtgaaagaa caagttcaaa gtatgggcgc
      901 ggaattcctc gagctggatt ttaaagagga agcgggcagc ggcgatggct atgccaaagt
      961 gatgtcggac gcgttcatca aagcggaaat ggaactcttt gccgcccagg caaaagaggt
     1021 cgatatcatt gtcaccaccg cgcttattcc aggcaaacca gcgccgaagc taattacccg
     1081 tgaaatggtt gactccatga aggcgggcag tgtgattgtc gacctggcag cccaaaacgg
     1141 tggcaactgt gaatacaccg tgccgggtga aatcttcact acggaaaatg gtgtcaaagt
     1201 gattggttat accgatcttc cgggccgtct gccgacgcaa tcctcacagc tttacggtac
     1261 taacctcgtt aatctgctga aactgttgtg caaagagaaa gacggcaata tcactgttga
     1321 ttttgatgat gtggtgattc gcggcgtgac cgtgatccgt gcgggcgaaa ttacctggcc
     1381 ggcaccgccg attcaggtat cagctcagcc gcaggcggca caaaaagcgg caccggaagt
     1441 gaaaactgag gaaaaatgtg cctgctcacc gtggcgtaaa tacgcattga tggcgctggc
     1501 aatcatcctt ttcggttggc tggcaagcgt tgcgccgaaa gagtttcttg ggcacttcac
     1561 cgttttcgcg ctggcctgcg ttgtcggtta ttacgtggtg tggaatgtat cgcatgcgct
     1621 gcatacaccg ttgatgtcgg tcaccaacgc gatttcaggg attattgttg tcggagcact
     1681 gttgcagatt ggccagggcg gctgggttag cttccttagt tttatcgcgg tgcttatagc
     1741 cagcattaat attttcggtg gcttcaccgt gactcagcgc atgctgaaaa tgttccgcaa
     1801 aaattaaggg gtaacatatg tctggaggat tagttacagc tgcatacatt gttgccgcga
     1861 tcctgtttat cttcagtctg gccggtcttt cgaaacatga aacgtctcgc cagggtaaca
     1921 acttcggtat cgccgggatg gcgattgcgt taatcgcaac catttttgga ccggatacgg
     1981 gtaatgttgg ctggatcttg ctggcgatgg tcattggtgg ggcaattggt atccgtctgg
     2041 cgaagaaagt tgaaatgacc gaaatgccag aactggtggc gatcctgcat agcttcgtgg
     2101 gtctggcggc agtgctggtt ggctttaaca gttatctgca tcatgacgcg ggaatggcac
     2161 cgattctggt caatattcac ctgacggaag tgttcctcgg tatcttcatc ggggcggtaa
     2221 cgttcacggg ttcggtggtg gcgttcggca aactgtgtgg caagatttcg tctaaaccgt
     2281 tgatgctgcc aaaccgtcac aaaatgaacc tggcggctct ggtcgtttcc ttcctgctgc
     2341 tgattgtatt tgttcgcacg gatagcgtcg gcctgcaagt gctggcattg ctgataatga
     2401 ccgccattgc gctggtattc ggctggcatt tagtcgcctc catcggtggt gcagatatgc
     2461 cagtggtggt gtcgatgctg aactcgtact ccggctgggc ggctgcggct gcgggcttta
     2521 tgctcagcaa cgacctgctg attgtgaccg gtgcgctggt cggttcttcg ggcgctatcc
     2581 tttcttacat tatgtgtaag gcgatgaacc gttcctttat cagcgttatt gcgggtggtt
     2641 tcggcaccga cggctcttct actggcgatg atcaggaagt gggcgagcac cgcgaaatca
     2701 ccgcagaaga gacagcggaa ctgctgaaaa actcccattc agtgatcatt actccggggt
     2761 acggcatggc agtcgcgcag gcgcaatatc ctgtcgctga aattaccgag aaactgcgcg
     2821 ctcgtggtat caacgtgcgt ttcggtatcc acccggttgc ggggcgtttg cctggacata
     2881 tgaacgtatt gctggctgaa gcaaaagtac cgtatgacat cgtgctggaa atggacgaga
     2941 tcaacgatga ctttgctgat accgataccg tactggtgat tggtgctaac gatacggtta
     3001 acccggcggc gcaggatgat ccgaagagtc ctattgctgg tatgcctgtg ctggaagtgt
     3061 ggaaagcgca gaacgtgatt gtctttaaac gttcgatgaa cactggctat gctggtgtgc
     3121 aaaacccgct gttcttcaag gaaaacaccc aaatgctgtt tggtgacgcc aaagccagcg
     3181 tggatgcaat cctgaaagct ctgtaaccct gacggcctct gcggaggccg tcactcttta
     3241 ttgagatcgc ttaacagaac ggcgatgctt tgacctcccg ctgtttgttc aagcgcaatt
     3301 ttgacaataa ttgttaacgg cacggaaagc agcataccca ccggtcctaa caaccatccc
     3361 caaaaaatca acgacaaaaa taccaccaat gtggaaagcc ccagcccacg tcccatgatg
     3421 cgcggctcca gaatattgcc gaagaccaga ttaatcagca gatatcccgc cagcaccagc
     3481 aacgcttcgt agaagccatt aaacaccagt acctgagcga taggggggat tgccgcgaga
     3541 actgaaccaa tattagggat gtaattaagc gcaaaggcca gcaatcccca gacaaaagcg
     3601 aagcgaacat cgagtgcggc gagcatcgcc caggcgacca ggccggtgat gatgctgatg
     3661 gctgttttca gcaccagata atgagaaaca ctgtcaattg cacgttgaat cgccgccatc
     3721 ccttcaaccg gacgcgccat catttgctga aattttccgg gcaattgtgg cacttcgagc
     3781 agcataaaca gcaccgtcag cagcaataaa aatattgatg acatggcatt agataactgc
     3841 gtcaataagt tggtgagcaa cgtcatcgcc gcgttcggat caatgtaatg cgccagctgg
     3901 tcaactgaga cgtcaatccc tacgcgttgc aacaacggct caagagcttg cagcggcgtc
     3961 ataatagagt tgcgatattg cggtaacgtc cgcgtcaact cgttgagcgc ggaacccaga
     4021 taagctaata gcaacaccat cgccatcacg atgatggtca tcaaaatcga caccgccagt
     4081 acacgcggca ctcgccagcg gaccatgtgt tgcaccagcg ggttaagaat aacagcaata
     4141 aataatgcga gaataaacgg cacgatgatc tcggcggcaa aacggatacc gcagagaata
     4201 atgaccagca ttcccaacat aatgacgatt tttaggccat tgagcgtgat gatcggcttt
     4261 gccatgcttg tccccatttt ttcttccttt gctaataata atgtcttttt cgctccgtca
     4321 tcataacgaa aatcaaaccg ctcaggtaaa gaagtgaaaa tattttgagt taattcttaa
     4381 gctatgatac aaatcaggcg tgttcaacta ccgaggacaa ttatcatccg cgatgacgag
     4441 aagcaacact gcggataatt gtaatattat ggacaatttg ttcaggacgt tattttcaac
     4501 atttacccat cttcgtacct catccactat cttgctcgta ggtgagcagc actggcgcaa
     4561 cgcgctttag tcctttcttc tgcctaagct tgccctatgc gcggggcttg accactttac
     4621 tttggtttcg ctgaattaag cgaaaattaa aataattctc ttgcaggaga aggacaatgt
     4681 atatttattg gattttatta ggcctggcta ttgctacaga aattaccggt acgctgtcaa
     4741 tgaaatgggc gagcgtcagt gagggaaatg gcggctttat tttaatgctg gtgatgattt
     4801 ctctgtcgta tatatttctc tctttcgccg ttaaaaagat cgccttaggc gtagcttatg
     4861 cgctgtggga aggtatcggt attttattta ttaccttgtt tagcgttttg ttattcgacg
     4921 aaagtttatc gctgatgaaa attgccgggt taaccaccct ggtcgccggg attgtgttga
     4981 tcaaatcagg tacccgtaaa gcgcgtaaac ctgaactgga ggtgaaccat ggcgcagttt
     5041 gaatgggttc acgccgcctg gctggcattg gcaatcgtgc tggaaatcgt tgctaacgtc
     5101 tttttgaaat tttctgacgg ctttcgtcgc aaaatatttg gcttgctctc cctggcggcg
     5161 gtgctggctg cctttagtgc gctttctcag gccgtaaaag ggatcgactt gtctgtcgct
     5221 tatgcattgt ggggcgggtt tggtattgcc gccacgttag ccgcaggttg gatcttgttt
     5281 ggtcaacggt taaatcgtaa aggctggatt ggcctggtct tgctgttggc tggaatgatc
     5341 atggtgaaac ttgcctgatg aacacgctgc ccgcactgtc gggcagcgtt tgaacattat
     5401 ttttgcgaca gttgatccag cttgtcgcga aaaccggtaa cagaaatggc ccggttatcg
     5461 gcgcgccagc gatctttcgc ggcaggagcc gaactttgca ccccaattaa ttgccagccg
     5521 tcatcggtat gcaacatcag aggcgaaccg ctgtcacccg gcaaggtatc gcactgatgt
     5581 gacatcactg acgtttgcgc ccagccagtc acttcacagt tttgatgact gtataacgta
     5641 tcgagatgat cttcaggata tcccgcctga gtcactttac gaccagccga ttttaatgcg
     5701 gcagtaagcg cggctttatc tccctcaaat aacggcaacg gtgtaatgcc agaaggggga
     5761 ttacgcagca caatcaagcc gaagtcccat ggtgcagctg cgggaggaac aatccaacca
     5821 tccccatctg cttttaaccg ctttcccagt gtcggatcaa cgcggccttc tatgtcgtgg
     5881 atgtcatagc gccaaagacc tttatttgac acaaaacgca gcgctaccgc cttatccgct
     5941 ttacccttag gcggcgtcaa taaacagtgt cctgccgtta atgccagatt gggtgcaatc
     6001 agcgtcgccg tacataaatt gccgctggcc gtttccagtt gcccaaccgc atcccacggt
     6061 gattgggtcg tgtcattcac tggcacacga tcatcatgac caaaaaacag ggtgctgacc
     6121 tcatcgtttg ccgatttggc aacgtctggt ttatctgcaa acacaaaagc agacgtcaaa
     6181 ctaattgcac ccaacactac agcaatggtt gtacgcatat cacactctgg tgggtaatta
     6241 tgattattaa aagcaaaccc tcataaatac tatagacggg acgggatgaa agtgggagta
     6301 aaatcagata actacagtca ggaaagataa aaacgggcgg caatcagtgc gcataaaata
     6361 aggatgataa gaatgaactc aaagcgataa cggcccacca tactaccctc cgctaaaggc
     6421 ggtgctaaac gttacccgct tagcaccgta attttcaggc gcgagggggc gcgccagcat
     6481 tactgttgaa aacttacgct gcgggttgtg cagcaggttt tgccggttgc tgatggctgt
     6541 gtttcccggc gtgtttcttc gctgcctgcg ctttttgttc aggggctttc tgttcagctt
     6601 tcgtattttt atgatgcttt ttagccgcct gcgctttctg ggcaggtgct gctttatgct
     6661 gttttttatg atgtgtagtt ttcgccggcg ctgctttggt ggtcgtcgca gtcggagcag
     6721 gtgtggtcgc agtctctgca gcaaaggcgg cagaagacag acccatagca gcggcaacaa
     6781 ccagacctaa tacttttttc attgtcatac cctcaatttg ttttttcatt taaccccact
     6841 gcggggccgt tgaaataact atatccctgg gaatggcaga cttccgtgag tggttggttt
     6901 cagcgtgtaa cgatatgtac aaacgctgaa taaaatacag cattgataat aacgtattgt
     6961 gacttaaggg aaatttagct accaataata gtagtcttga tcgggttaac tattttacta
     7021 cttacaaagc ggtagaataa ctgcgcatca gtaataaatg acacacagca aaatgaatcc
     7081 gtttatttgg gtacttataa tcctgatgac gctagacgcg ctgcgggaat tggctggcgc
     7141 ttcgtctatt ttaggatggc tattaacgtt ggtttgagca agcaataagt ccggacgtgt
     7201 agttaccgca gtccgggctt atttttcagg cgtgcagacg acgatgcaaa cgcgtcccga
     7261 ccagcagagc aatgaccagc atcagagcaa taaatgcgcc gacgccgttc cagccatagt
     7321 tatgccagaa aacaccaccc agcgtcccgg caatactcga ccccagatag taactgaaca
     7381 gatacagcga ggaggcctgg cctttagcgc gttttgcgcg ggggccaatc cagctgctgg
     7441 caactgagtg ggctgcgaag aatcctgctg agaagagtaa cattccggca aagatcagcc
     7501 acagcgagct gaataaggtc atcagtaaac caaacagcat aacccccgtc gaaaacaaca
     7561 tcaccggacc gcgcccgtag cgggtggtca tggttccggc tttaggtgag ctccatgtac
     7621 cggtcaaata agccagcgat aataagccaa ccacggcctg actgacatgc cagggggaaa
     7681 gcatcaaccg atagccgatg taattaaaca gcgtgacgaa cgaccccatc agcaaaaagc
     7741 cttctgcgaa caataacggt aatccccggt cacgccagtg cagacgaaag ttgataaaca
     7801 acgtcttagg tcgcagcgaa gtcgggcgaa aatggcgtga ttcagggagg attttccaga
     7861 acatcaacgc cgaggctagc gcgaaacaac cgattgccgc cagagcaatt cgccagttga
     7921 aaaagtccgt gaagacacca ctaattaagc gcccgctcat gccgccaatt gagttgccgc
     7981 tgatatacaa ccccatcgaa aaggccacga aactggggtg gatttcctcg ctaagataag
     8041 tcatgccaac agctgccacg ccacttaacg aaagcccaat caaggcgcgc ataatcaaaa
     8101 tgccgtgcca gctggtcatc attgtcgaaa gtaacgtaca aatggaggcc aacagtagcg
     8161 ccgtgaccat cactggtttg cgaccaatgg catcggatag ggggccagta aacagcaaac
     8221 caatagccaa catcgccgtg gaaatggaca gtgaaatact actgttcgcg ggcgttaagc
     8281 caaactcctg cgaaagcacc ggaaggatag gctgcacaca atagagaagt gcaaatgttg
     8341 ccagtccggc agagaacagc gccagggtga cgcgcataaa ttgcggcgta ccgcgtttaa
     8401 taaattgatt tggctgagaa atgctttgct tgtcagtgtc gcttgccgga gcgccatcaa
     8461 cagttgtagt acggctcact tgaaatcctt gctaaatatg cctgtggatc aggcttatac
     8521 atagggtagg aaaatcgaat tgttctgtct aatatattaa taatctcaaa taagatgttt
     8581 taaatatgaa tattgaactt cgtcatctgc gttactttgt tgctgttgcg gaagagctgc
     8641 attttgggcg cgcggctgcc cgcctgaata tttcgcaacc gccgctaagt cagcagattc
     8701 aggcactgga gcaacaaatc ggtgcccgac tgctggcacg aactaatcgc agtgtattgc
     8761 tgacggcagc aggaaaacag tttcttgcag atagtcggca aatcctgtct atggtggatg
     8821 acgctgccgc tcgcgctgaa agactgcatc agggtgaagc gggggagttg cgtattggtt
     8881 ttacttcgtc cgctcctttt attcgggcgg tgtctgatac gttatcgctg tttcgccgtg
     8941 attatcccga tgtccattta caaacccgcg aaatgaacac tcgtgagcaa atcgctccgc
     9001 tcattgaagg aacgctggat atgggattgc tgcgtaacac agcgttaccg gagacgcttg
     9061 aacacgcagt catcgtccat gaaccgctta tggcgatgat cccgcacgat catcccctgg
     9121 caaataaccc gaatgtaacg ctggctgaac tggcgaaaga acccttcgtc ttttttgatc
     9181 cgcacgtcgg gacagggctg tatgacgata ttctcgggct gatgcgacgt taccatttga
     9241 cgcccgtcat cactcaggag gtgggcgagg caatgaccat catcggtctg gtttccgccg
     9301 gtctgggtgt ttcaattttg cctgcgtcat ttaaacgtgt tcagctcaac gaaatgcgct
     9361 gggtgccgat tgcagaagag gatgcggttt ctgaaatgtg gttggtctgg ccgaaacatc
     9421 atgaacaaag tccagctgcg cgtaactttc gtattcatct gctgaatgct ctcaggtgag
     9481 ggaaatttca gcgaaaaagc ccgaaaaatg tgctgttaat cacatgccta agtaaaaatt
     9541 tgacgacacg tattgaagtg cttcaccata gcctacagat tatttcggag cgcgaaaata
     9601 tagggagtat gcggtggttg ctgaaaacca gcctgggcac attgatcaaa taaagcagac
     9661 caacgcgggc gcggtttatc gcctgattga tcagcttggt ccagtctcgc gtatcgatct
     9721 ttcccgtctg gcgcaactgg ctcctgccag tatcactaaa attgtccgtg agatgctcga
     9781 agcacacctg gtgcaagagc tggaaatcaa agaagcgggg aaccgtggcc gtccggcggt
     9841 ggggctggtg gttgaaactg aagcctggca ctatctttct ctgcgcatta gtcgcgggga
     9901 gattttcctt gctctgcgcg atctgagcag caaactggtg gtggaagagt cgcaggaact
     9961 ggcgttaaaa gatgactcgc cattgctgga tcgtatcatt tcccatatcg atcagttttt
    10021 tatccgccac cagaaaaaac ttgagcgtct aacttcgatt gccataaccc tgccgggaat
    10081 tattgatacg gaaaatggca ttgtacatcg catgccgttc tacgaggatg taaaagagat
    10141 gccgctcggc gaggctctgg agcagcatac cggcgttccg gtttatattc agcatgatat
    10201 cagcgcatgg acgatggcag aggccttgtt tggtgcctca cgcggggcgc gcgatgtgat
    10261 tcaggtggtt atcgatcaca acgtgggggc gggcgtcatt accgatggtc atctgctaca
    10321 cgcaggcagc agtagcctcg tggaaatagg ccacacacag gtcgacccgt atgggaaacg
    10381 ttgttattgc gggaatcacg gctgcctcga aaccatcgcc agtgtggaca gtatacttga
    10441 gctggcacag ctgcgtctca atcaatccat gagctcgatg ttacatgggc aaccgttaac
    10501 cgtggactca ttgtgtcagg cggcattgcg cggcgatctg ctggcaaaag acatcattac
    10561 cggggtgggc gcgcatgtgg ggcgcattct tgccatcatg gtgaatttat ttaacccaca
    10621 aaaaatactg attggctcac cgttaagtaa agcggcagat atcctcttcc cggtcatctc
    10681 agacagcatc cgtcagcagg cccttcctgc gtatagtcag cacattagcg ttgagagtac
    10741 tcagttttct aaccagggca cgatggcagg cgctgcgctg gtaaaagacg cgatgtataa
    10801 cggttctttg ttgattcgtc tgttgcaggg ttaacatttt ttaactgttc taccaaaatt
    10861 tgcgctatct caatttgggc caggaaagca taacttagac tttcaaggtt aattattttc
    10921 ctggtttata tttgtgaagc ataacggtgg agttagtgat gctgaagcgt ttctttatta
    10981 ccggtacaga cacttctgta gggaaaacgg tggtttcccg cgcattgcta caagcgttag
    11041 cctcccaggg aaaaacggtt gcgggatata aacccgtagc gaaggggagc aaagagacac
    11101 ccgaagggct gcgtaataaa gatgccctgg tgttgcagag tgtttcaacc atcgaactgc
    11161 cttatgaagc agttaatcct atcgcgttaa gcgaagaaga aagtagcgtg gcgcacagtt
    11221 gcccaatcaa ttacaccctc atttcaaacg gcctggcaaa cctgaccgaa aaagtcgatc
    11281 atgtcgtggt agaagggact ggcggctggc gcagtctgat gaatgatttg cgtccactct
    11341 ctgaatgggt ggtgcaggaa caactgccgg tgttgatggt tgtcggtatt caggaaggtt
    11401 gcattaacca tgcactgcta acagctcagg cgattgccaa cgacgggctg ccgctcattg
    11461 gctgggtggc taaccgaatc aacccaggac tggcgcatta tgcggaaatc attgatgtgc
    11521 tcggtaaaaa acttccggca ccgctcattg gtgaactgcc ttatctgccg cgcgccgaac
    11581 agcgtgaact ggggcaatac atccgcttag ctatgctgcg cagtgtgctg gcggtagata
    11641 gagtcacggt gtaacgtccg cgagattacc gacgcaatta cgcaggcaat caataaaccg
    11701 gggagtagct gatactcccc ggtcatttca catatcatca acgtcgacat aatcggcgcg
    11761 tgcgtggttg ccgccaacag tgtcgccatc ccggtcaatc cgagtaaaag cgtaatttct
    11821 tcgccatcag ggaaccataa tcccaggcta cgaccataca acatgccaat ggcaagaccg
    11881 ataaatagcg tcggtgtaaa gacaccaccg ggtgcgccgg aaccgctact cgccagcacg
    11941 gcacacagtt tacaaaggaa gatcccggca atgatcatta acagtggcgg ggcggttaaa
    12001 aaggattgta cggtgctata gccgttgccc cacactgcag gtgtgaacag ggaaagcaga
    12061 cccacgatca acccgcctag tgccagttgc cagggcggcg caagtttgag acccacaaat
    12121 ccacgatgac aggcgttcat taacgttaac aacagtggtc cgcacagacc tgccagcaca
    12181 cctgtactga taatcagcgc atagtcacga gcctgaaccg tcactgagag ttgtacgttg
    12241 tagagtaacg cgtcgctatg attaatcaga ttgctaacca gcaatgccac gacggcggaa
    12301 ataatcaccg ggccgagaga ggccaacatc atagtgccaa acagcacttc ggctataaat
    12361 aaactgccag caagcggggc acgataggcc gcagccattc ccgccgcggc cccacaggcg
    12421 atccataatt tccactcctg gcgtggcgta aaacgttggg caaaacagga ggcggcaagg
    12481 gcagctaaaa gaatcatcgc accttcgcga ccaattgcac tgccgctggt tactaccagc
    12541 agagaggcaa gcgatttaac caggcttgct gcgtaatcga actgtccatc ggtttgcaac
    12601 gcttccatgt aatcggtcgg cgcatgaggg cgttgttggg taaatttctg ccagcccatc
    12661 agcaacaaac ccgccgccag tccgccgagc gccggagtta gcaaccgtcg ccagggggaa
    12721 aggtttgtcg ctgcattgac cagactgccg gagtcattat tgaggaacaa ccactccagt
    12781 agcagcatcg catgacgaaa cccggcaacg gcaaaggccg cgagaatacc gacgactgtt
    12841 gcgataagca gacggcggaa catagtgcgc agatccggat agatgtgtag atgatgcatg
    12901 agcagaaatg ggcaatgaaa cacgggcgac tattttgcgg caaaagaggc cagccgcaaa
    12961 agcagtgcgt gctttaagag ataaaaaaac gtgacactgt aacctattat tgccggattg
    13021 cgcgtaatcg tcaccatccg gcaatattac ggtgatccta tcgaaacagc ggtttaaccg
    13081 cgacaggaat taacagttgt gattgccatt gcgccagcgt taagcgagcc agttcaccca
    13141 gtgcacggta gaaagggtgt tctgcttttt cgataaaaac atcaagaaaa cgtgttgacc
    13201 acggaaaaag gtgccatgcc agcaattctt cgcattctgt ctggcgacca ttctccgcca
    13261 accacgcagc catcaacagc agtgaaccaa aatgatcttc cggttcgttt tgcttcattt
    13321 caaactgaat gcctttctcg cgcatccact gacgaagtgc caatgttgaa tcgccaaaca
    13381 gcacagattc gcgatccagc cagaccgacc cccacggcgg agacggcagt gcccacgggc
    13441 cgacaaacaa acgctgccag gcctgggcgt gagtctcttc acactgtgtc tgaaaagccg
    13501 tcaccagcgg cgctaatgac gcttctggta gaggccactg agtttcccag ccgtcactgg
    13561 tgagtaccgc aaccagaggt gcggcttccg cactctctgg agcgtaataa aacagcgcgc
    13621 ccaacacgcg cgccgcgaca gaaaaattat cttgctgtga aaaatgggtc attcctaaca
    13681 tcctgaaatg tgcgggttgc cccgcaccaa tttgttaacc tgcaatagcc attcctactg
    13741 tcatgtgcag gccataaaag agcacacgcc cgatcatctc accgccaagt atgaggatta
    13801 gccccagaat aagtccggca acgtgaggtt cacggcgacg aatgagcggg caaagccagc
    13861 atccaagacc tgcgcataac aataccacac gccagacctg taaggaggcg taatctggta
    13921 ccagtgcgct ggcttgttgc acggatgagt gaatagacgc cagagacaaa ccttgcagca
    13981 caatcacccc ggcacaagcg atcagcgcca gaacgctaat gatggcaaat ggtgtggtat
    14041 taaatgtaac gcgggctgcc cgcagaattg cggcagcaag tattggaccg ctcaacaata
    14101 ccgtcaggaa gaaagccagc gtagtgtaac cgttatgcca ggttggcaca gtgtcgattt
    14161 gatacacgca ggtcatcatc cagacgaaaa tgacgccgag cgccatgctg acaagtaacc
    14221 agagtttccc caacgcttgc ggcattttac cgatgaccgc caccagccac catagtccgc
    14281 caacggcaaa aaatatggaa ccggcagcaa tctcgttact cagaccagaa gctccgattc
    14341 gattcagcga gttaaacgcc cgcagtggcg atcccaggtg catgacagag gcaataaacc
    14401 cgatgcccat caacagccag aggaaaaaca tgccacggac aatacgctga cggtctgcgt
    14461 catttttggc ggcgaaccag ccaattccgc taacaatcag cgcgcctact acgcattgac
    14521 ccaatacggt aaagataacc agtggccact catgccatcc atttcccatt ttacacttcc
    14581 tccggattag ccagataacc agtggtatcg ccggtcgggc ggctgttggc gttaggtttg
    14641 ataacgatat tgggttttgt gaaatgcgcg cgcggcagtg gcgcgacggc agccagagtg
    14701 ccgtgcttct ggcgcagctc ttcaattgga ccgaactcaa gtgcacgcag cgggcaggat
    14761 tcgacacata tgggttgttt cccctcggcg acgcgcgaat aacaaccatc gcacttcgtc
    14821 atgtgccctt tttcagcatt gtactgtggc gcgccgtacg gacaagccat gtggcagtag
    14881 cggcagccga tacaaacatc ttcatcaacc accacaaagc catcttcacg cttatgcatt
    14941 gcgccgctgg ggcagacttt tgtacatgcg gggtcatcgc aatggttaca ggagatggag
    15001 agataataag cgaacacgtt ctggtgccag acgccattat cttcctgcca gtcgccgcca
    15061 gcgtattcat aaatacggcg gaaactgact tccgggccaa gatctttaaa gttgttgctc
    15121 accacttgaa agcgtgagct taactcattc taattcaatt gcttatgctt tctttgcatc
    15181 ctcaaaaacc tcagggtggg acaaatttgg gacatcgtca ctcaatatgt cgtcaatttt
    15241 cctcgcgtgc tctgtcaaat gattaggtgc aaggtgtgcg tacctacgca ccatttcgat
    15301 agactcccag ccgcccattt cctgaagtac tgacagcggt acacctgact gaattaacca
    15361 actggcccaa gtgtggcgga ggtcatgaaa acggaaattt tcaattcctg cccgacgaca
    15421 tgctgttaac cacgatgtgt tatagtccag gcgcatcttt ctgatgcttg gtgtcattgt
    15481 cccgtcaggc cttctggcag cagtggtata aacaaacacc cagcggtgat gtttgcctat
    15541 ttgatcacgc aacaccttgc aggcagtgtc attcagtgcg accccaatag cgcggtttga
    15601 cttactgtct tcagggttta cccaagcaac acgtcgctgc atatcgattt gttgccactc
    15661 cagattaata atgtttgatc tcctcaggcc agttgccagt gcaaatttaa ctacagattt
    15721 caggggatca gaacatgcat caatgagtct cctggcttct tctttttcca gccatctcac
    15781 gcgtttgttt tttacggcag gtattttgat tacaggtgct ttttcaagcc atttccagtc
    15841 tcgctctgcg gcgcgcagaa tagccttgat cattgccagg tgtgttgctt ttgtttgcgt
    15901 gctcaccgat ttaggtatat aggctggtgg ttctttccct tttcttaatg cagcctccac
    15961 ctgcaacttc catctctcct ttgtttttcg gttatacgct ttgctgataa ctgagtagat
    16021 cattgcctcc gatatatcct taatccttat tccctcgaaa tgctcaatcc agaatgcgat
    16081 tctggattta tcggaatcga tggatttctt gtcggctttt tcctcaagcc atctcaggca
    16141 ggcttcttcg aaagtgacat ctggcatatc ccccagtctg tctactcgcc agagttctgc
    16201 ttttcgcttg tcgtgcaact cctgagcttg ccgcttgtcc tttgtgccaa gagactcctt
    16261 aattcgcttc ccgcccggga gcgagtacga ggcgtaccat atttcacctc tgcggaagag
    16321 tgacattttc tttcctctgt tatgccatca cccgcgctca cctggacagt atgcagcggt
    16381 gaatgaagtg ctgcaatgca ggcttgccgg gttgtgagat aaggtgatct tttaccggaa
    16441 gaattttttc gtgttgcctg tagtcggccc gttcgtatcc agttaacagc tgttggcctt
    16501 gatatcttga gaaactgaca ggcctctttg agggtgatac tgtgtgaatc catatttttg
    16561 ccattaaaaa ccgcccgaag gcggttgtca gttgattgat gtacgtcgca tttttcgaag
    16621 gctggcaata tgcatttcct tctcaatttc cgctttaatc atgtgtagtt cgttgtggtc
    16681 gactcgctca aattcttcat taaatgcacc aattgaagtg gctcgggttc tgccgtcgag
    16741 tcttcggtag atcacctgag taagagttac cttgcagatt tccaccggat agttgttggc
    16801 atcgacgaaa gattgcccgc gctggatcag gacgaacacc ggtagcattc cttaactgtc
    16861 atatcatttg cacctcgttg ctacggctat cgccattgct ccccaaatac aaaactaatt
    16921 tcagccagtg cttcgtccat tttttcgatg aactccggca ccatctcgtc aaaaccagcc
    16981 atgtattttt catcccgctc gaccacgaca taatgcaggc cttcacgctt catgcgcggg
    17041 tcatagttgg cgaagtacca ggcatctttt cgagtcaccc acatgctgaa ttgcacctgg
    17101 gccatgtaag ctgattttat ggcctcgaaa ccaccgagcc ggaatttcat gaaatcccgg
    17161 gaggtaaacg gacatttcag ctcaaggccg ttgccgtcac tgcataatcc atccggagag
    17221 caggcggtac gcatactttc gtcgcgatag atgatcgggg attcagtaat attcacgccg
    17281 gaagtaaact caaacagggc tctggcgtcg ttctcgtact gttttcccca tgccagggct
    17341 ttggcgttaa cttccggggc cacacccgtg cagacctcag ccagcagggt gtggaagtag
    17401 gacattttca tgtcaggcca tttctttccg gagcggggtt tggctatcac gttgtgaact
    17461 tctgaagctg tgatgacccc gagccgtaat ttttgccatg cataatctcc ctgctcgaca
    17521 cctctcacgt cgatcccggt acgttgtagg ataatttctg gtgtcatgct gccaccttct
    17581 gctccgtggc tttctgtttc aggaatccaa gaaccttctc tgcttcggcc tgggacagtt
    17641 ctgacgacgc ccgaatgtag cggcgaaata tctgagaaca gagcggcaag aggtcgtcat
    17701 cccacgtttt ttccatggaa gtaagaaggg cgttaatttc cgacatggtc tcttcgttaa
    17761 ccggggtgat gtcgcgttcc ggctgacgtt ctgtagtata tgcggtattt tcgacaatgc
    17821 gttcggcttc atccttgtca tagataccag caaatccgaa ggccagacgg gcacactgaa
    17881 tcatcgcttt atgccgtaac atccgtttgg gatgcgactg ccacggcccc gtgatttctc
    17941 tgccttcgcg ggttttgaat ggtgcgcggc gacattcatc catccactcg gtaacgcaga
    18001 tcgggtgatt acggtctttg cggtaaatcc gacatgtaca ggattcatcg ttctgctcaa
    18061 agtccatgcc atcaaactgc tggttttcgt tgataatgcg ggaccagcca tcaacgccca
    18121 ccaccggaac gatgccgttc tgcttatcag ggaaggcgta aatttctttc gtccatggat
    18181 taaggccgta ctggttggca acgatcagca atgcgatgaa ctgcgcatcg ctggcatcac
    18241 ctttaaatgc agtctggcga agagtggtga tcagttcctg tgggtcgaca gaatccatgc
    18301 cgacacgttc agccagcttc cctgccagcg ttgcgagagc tgtactcatc tggtttatac
    18361 ctctgaatca atataaatct ggtggcgggc aatggtttca accatgtacc ggatgtgttc
    18421 tgccatgcgt tcctgaaaat cgacatcgtc atcaaatgca cgggcaatgg cttttttgct
    18481 ggctccatga cgttgcagat tatcgatgca cagcgattca aacaggtgct ggggcagacc
    18541 tttttccagg tcgtccgcca gttcagtctc attttcttca cgggtaattt gccggtagtg
    18601 tcgtgcccat gactgctctt caatgcgatc ggggataagc caggcattca tgatttatca
    18661 cctccaaaat tttcaagcct gttggcaatc atgattgcga tatcagggat tgctggcgct
    18721 gttgcgacgc atgcagggtt tgcgcacaaa ccatagacgg ctgcaagcac gagttgcctt
    18781 ttccagtcga aatctgcttg ctctgaatca tcattattgt cagaagcatc actatctgga
    18841 tcgatgtgaa caaccgaatc gtgttcctga gttgtatcaa cagcattttt ctgaatgtat
    18901 tcattcttca tttgtgcctg gtcatctcca gcagcggtct cattttctcc aaaagtttca
    18961 gcgtaagttt catctcccat tactggacca cagtcagggc aatgcccgcc actgttctga
    19021 ccgcatgcgg tgcaaacctt ttccacttcc tgttgcatta ctggcccagg ctgttgctct
    19081 tccggtccgt tttgttgcgt atccgggctg ttttgttcca ctttggattg attctggtcc
    19141 tcagtgtcgc gagtctggat ccccttcacc cacttcgggt cggttgggtc gctgatgcct
    19201 tcaacgaact ccccacgaga ggcagccagc aatttgtctg catcgacagg atttttgggc
    19261 aaaatgtttt tcctggcttc atagagttct gctctcagtt cctggtattt cgtttctaca
    19321 gatgagacat tttccagtga ttgcgtgtcc gcattatgtt taactggaat ttcttccact
    19381 gattcaggcg ctgcctgttc attagacatt gcgtccgatg tttgctgctt ttcttcatcg
    19441 tcatgtatct cttctgctgt tccgcgctgc ggcatcgggg ctgatgagcg accgcaggca
    19501 atttccacga tttccggatc cgggttagag tgatcggttt cggtcaacac tttgttgaga
    19561 tattcagtca cgcgcgccgg gatagcctca atgccaattg gtgcttcttt cactgaagcc
    19621 accacaatgg cgcgggaata atccagccca ccgggcatgg cgatgaattt gtcacggaaa
    19681 acagaaaagg gcggtttatt ctctgcaacg atttcttcaa cgcgttttgc atgtgcaggg
    19741 tgcaggttat aaatatccac atccattgaa cgggccagaa cgccagtggc aatgtctcgc
    19801 gccagtgatg cctgattgtg ggtaaagcct tcgccacgat cggtaagatt tccgccgcca
    19861 gcgttagcac cggaaggcgt acgagtgatg cctgtaacat aatttccttt ctgccattct
    19921 tttgccagca atccctgatc cacatagtct gttttcatcc aggtggagat gaacttgtcg
    19981 aattcggccg ggctgatatg acggggtgtt gcgtgagaga atgcctttcc aacggattgt
    20041 gccagtcttc ccagatgata attcgtcagc ttatccagct ctggatgtga acgcacagca
    20101 gtaagcaggc tctgaaggta actgtcttct gtgtccatct ccatacggat cacgttattt
    20161 cgttgttcgg gtgtggcgtg atgccgatat tttccatctt catctttgct gaagaagagg
    20221 tgaaggaagc gatgagtaag gctcagagtg gcgacgggaa tttcacactc agaacagtca
    20281 tcgtcgctgt ccggggattc gcttttctcc acatcatccg gaatagttcc gtccaggtta
    20341 tcgtagtcat cgccagctgt tgtggcatct tcaccgttga tgttgtcatc gaaggggatt
    20401 gccatcatgg tgatgccatc ttccccgcct ttttcatagc ggttgcagaa ttcagtatca
    20461 aacacgcctt ccggtggaag gtcattcacg acggggaaat ttacgcgaac gggttttttg
    20521 aaatcatcct cgtcgtagcc tgcatcgtcc atggctgcaa tgcagcggga aactgcgaca
    20581 gaaagttttc tggtatcagc ccagaaaaaa ccgcctttga tgccaaggcg ttttctgact
    20641 ttatcgtttt ttgcttcgca gtgcagtgca aaagtttgtt tatcagcgct cattgtattt
    20701 aaacctctgg ctggattaga attagcggcc ctttgtctga tttctccgaa tacagtgacg
    20761 caggggggag tccggtagcc tgcgctgccg gattttttat ttcagtgtaa ggttgcctgg
    20821 tttgttgttt tgtgagttgt ttttttcttt tcatgctggc agtcagagca gttgcattcg
    20881 ccattttctc tgagaagttg aagtgccagg gtcagttcct gaattctttg tggatcaata
    20941 tgactgtcga caatttcaga tgcagcatga atatatgggc tggtggtttc gcaatataaa
    21001 ccgaaggaaa caaagcgact gtctttatat tcgttatttc caacttctgt aagcactgaa
    21061 aaagaaaata caaagtcgat tttgtgtttt ttgcatattt tactgatgcg ttctgcaatt
    21121 tctttgagtt catttattgc atcaggactg gtttcggaaa aaaattcatt ttgcgcaagt
    21181 tctttcattt tgattttccc gtgattgata attaatgtgg tttttatttc ggtttgtctg
    21241 ttacagatat ggaaggtgta ttttattact cgtcacaact actctgcttt tacaggtaag
    21301 ccatcgcgcc cgatgaaaac tttaatcatg cagtcggtaa tgcatgtttt tgtagtcagg
    21361 ctacgaatat agagctttct cttttcaata ttgtttgctg aagcgatata tgtgcggcct
    21421 tcatgaagaa cataatcgcc aggtttcacg cactgacgtg gtatttcatc agttccgaag
    21481 tgatgagcaa tcataattat ctccattttc acaaatgaac tttgttgatg cggtgcctag
    21541 tgcctccagg tgacgttaac cagttaacaa ttaacgccgg agcaggggat gatgattttc
    21601 cgtgacatcc tgtcggttta actgttccgc gtgcgcatag ccgcattcac cgcatcataa
    21661 aattcacttt taaaaagggc ggacatcagc aatcggcaaa ccgatgtccg ccaaagagga
    21721 tattgataca tggatgttgc agcggggttg tcactcaggc gtctggggcg atttccatta
    21781 tttacgatgc acttgaaaca ttccttgatc gaaaactttc atctgtcacc gacggaacat
    21841 cgagtaagca ataacattta acacacactc taaataacaa ggggtgggct tgataccgtt
    21901 cttttcaagc tcactcgtaa tcacctccag aagtcgtgcg cccatgagaa ttgtcttgtg
    21961 ggatgaagct attacgccgt tgctgatatt gctgtttatt tctattgctt cattttcacc
    22021 attcgcgcta acttcggata tgttattttc actctcctgt ttcagagctt tttctgccat
    22081 ccggatgcgt tcctgcgttg cggagtttgg gctaagtcga taaacctgtc tggcatcttc
    22141 cagaagcagg gcgataatgt gcttcagttc tgtttcgttc atagttaact ccggtagttg
    22201 caatttatta atatcaggcg gtcagctctt taagcttctg aactgcttta ttcatttcat
    22261 ccatacagtc gatgaattcg tccagtttaa gctgcatttt tccggcggcc tgaagaattt
    22321 caagttttaa gggcgcaagt tttttgttga atacatcatc ttcctggcgt tttttctccg
    22381 caatttcggc ggctttcagc aattcttccg cctgctttct gagtatttct ggtgaaggtg
    22441 aaactgcttt taggttattc atttcaggtt cctgttgttg cagaattaat ttgttgcatt
    22501 ctctgcgtga gcgtttcatc gcagattcgc acttcttgca ataaatggct ctcccgtcac
    22561 gtttgctggc atctgaataa aaatcatcga ctgattttgt ctgcccgcat ctggtacata
    22621 attttgattg aaggtcctta atgattgtga cgtttccttc ccgcttggga tatccatatt
    22681 ttttgtggcg gaaaaaggtt actccgttac cagaaccttc ttttctgacc ttaaatacaa
    22741 tgatgctttc ccgtttatcg ataaaatcta tgagctggtt tctctccttt cctgacagtt
    22801 cggcaaatgt cttaactttt ttagtaactt ccgtaaggcg aacgccgtca ggcatttttt
    22861 caacgaagtt tttaatctct gcaagcggac gccataattt ttgcggcaaa actgaaggcg
    22921 tggtcatcaa tcacctcgct tttttttact gatttgttta tgccaaaaat aaaggccacc
    22981 atcaggcagc cttgtttttc tgtttgctaa gttctctggc aatcatggcc gtagttcgta
    23041 ttgcccacac cattgatttg tatcaatagt cgtagtcatc cggatagtcc tggtattgtt
    23101 ccatcacatc ctgtggatgc tcttcgaact cttcaaattc ttcttccata tctcacttca
    23161 aataagtggt ttgctgccta attttatttt ctggcgacca acacaagtca cacccgtttc
    23221 actgcgtggc ttgcggtagt aaattttgtt agtccaaaca ataaaatcta ccgaagtggg
    23281 ttatgaccat tttttatttg gatttcgttg gtgagcgtgg ttaacaactc tgtgcattat
    23341 atcctcatat ttttcatctt taattttttc aacatctcga ggaaatggtg ttgctaatgc
    23401 tttgtcaact ttgtccattg ggtcttcatt aatcttatat tccggaccgt catctatagc
    23461 attaaatcca ggtgttatac cgttttctaa tgcatatgct attctctttt cccatctcgc
    23521 tatcctcctt ctgtctcgag atgtaaggct tctatcagat acttttctgt tttgtccgcg
    23581 attaggatta acataaatag tctttttcac cataagcata ctcaataagt ccatacggtg
    23641 gtttactgta caattttatt ttttgaactg catgtatttt gtttcctaat gggtttgaat
    23701 ccttgtaata agtacttctg tttttgcgct cgacttcttc tgccttcttg ttgcgaaggc
    23761 tgccgagtga tgctgctttg tctgctctga cgcaaccaga gatctttagc gcaatcttcc
    23821 gcgtcagtct ttcactactg cgccgctctg caataagttc ttccctgcga gctttatagc
    23881 ggctttttgc cgtacctttg gattctttcc agattatggt taccatgatg gtctccttta
    23941 agtggctttg gtgtatgacg cgtcgaggtg tttttcttct cgatcgcggc cttgcaactg
    24001 aaattcgcgt catccccaaa accacttaga ttttggtctc aacggttagg ttgagagtcc
    24061 atcaatgtta aagagcctgc caatctgttc cgtttggctt ccagcgtcct gctgttgaat
    24121 tgaagataac ctaagttatc tgattgtgca ataactatat ttatcatttt atggaaaacg
    24181 ttataaatgg ctgataacaa aaatatttta ttttttgtgg tatccgcgtg atattttcaa
    24241 gggggaaagg ctgattgtta tgggtgattg catgttaatc gaaggggaat ttggtgtgtt
    24301 gcaccagcgg gtagtcgaaa ttctaggggt gtcgttgctt gaggttattg ctactgggga
    24361 agctatttca gcagatgcta ttgcgggaat gatccgagtg cttcaccatg atgaattgga
    24421 tgatctcgct gtgaagttag ctatagatgt gttacttcag gatatgcgac tgtgtaatta
    24481 agtaaataaa acccggcact ggagccgggg gatttagaaa tggcatttta tgaattggct
    24541 acttggcgaa tggtttacca tcggtggtat tgagcacaat aaaaggccgt tcttcgtgag
    24601 tgttcttata gcaaaagttc agttggagtc tgattaccag ctatgtgatg accagaacac
    24661 tcgtcctatg atttttacgt ttttgtagaa ttcttctctg ttcattactt catcaggata
    24721 ctcttcgcgg tttatcgatc tgataattac cgatgttggt gtggctatta gcgtttttac
    24781 ccttaacaaa tcagcttggc aaattgcata tgttttacca tccctgatgc tcgtatcttg
    24841 cgtgtttacc cccaccacat caccatcatg gagtgttggc tccatacttt gcccaacaac
    24901 cctgaccaac tttgccgctt tttctggaac tcccattttt ttcaggtaat agcgcctaaa
    24961 aactagagag aattctgcgg actcctccaa tgcacaactt cctccgccag ctgaaagtga
    25021 aatatttaga agggggagcg caacaaattc gtcattatca ttttgatgat catcccaggc
    25081 gatagctttt aaagatgatt cccgagcatt agatggctct tctgcgctct gtggtctcat
    25141 tgaccctata ccagagctta gccactcagg gcgaactctt aacgcgttgg ctaattcgac
    25201 cattttacgt gatccggttg ttttaccaga tgacatcttt tgtatggctg gttgtgatac
    25261 ccccaccatg tctgcaagtt gtgcttgtga caacccggct gaactcatgg cggcatttag
    25321 tctttctgcg aatgttttca tacctgtaat ctataaccac ggttatcaaa agtaaaacaa
    25381 caattgttat tgctctgttg tataactcat gttatttttg gttatgcttt atttgctgta
    25441 gaggtatgct catgaattta gtgattcagc gagccttgaa tattgttggc agtcaaaaac
    25501 gacttgcagc tgattgcggc gtatcacagc ccgctgttca taaatggttg cgaggcggaa
    25561 aagtttctcc tgaaaaagtt ttcgctatcg ttaatgccac caatggtcag gttaaggctt
    25621 acgaaattcg cccggactta ccgcacctgt ttcctcatcc gaaccaggct gaataagtaa
    25681 caccgctctt taacattgct ggtcgttcac ctctaacagg gtgagcaaac atcagtggca
    25741 aacccattgg gattgccgct taaccccata tcaatatagg aaaattaaca aatgtcacaa
    25801 acaagttaca gcaaactgtc gcagcgcgat atcgatcgcg ctgaaacgga tttacttatc
    25861 aacctgtcag ttctgacgca aaggggactg gcgaagatga ttggctgcca tgaatcgaag
    25921 gtcagtcgta ccgactggcg atacatcgcg gcgattttat gtgcgttcca gatggcatct
    25981 gatatcagtc cgatcagccg ggctttccag catgccattc gcgttcatgc aaataaaaaa
    26041 cgtccggttg gggccggacg ttctgagcaa atcctgatga atatctgata ttcaggcagg
    26101 gcatggagca atacacggga ataattctgc cacatctgga agaatttcgc cagcaacaac
    26161 accaaccgca gcagcctgaa gccgattggg ttaacccggg agataccggg accgtctgcg
    26221 gtatggagta aatcttgtat gcgaggggac tatgcgtaat tacgcaacaa tttcacctca
    26281 gttctggtta ggcgatacag ggcgaaaact aaggaagtct ggtccggaat gtatggtagt
    26341 ggcgttgtat atgatgacct cgcctcattc caatatgctg ggcctttatt acctgcctgt
    26401 tttgtacatt gctcacgaaa ccggacttga tcctgaaggg gcttctaagg ggcttcaaat
    26461 ggcttgcgag gctggttttt gcagctatga ccatgattct gaggttgtat gggtgcatga
    26521 aatggcagca tggcaggttg gtgaagcgct gaaaccaggc gataaccgtt gtgctggggt
    26581 aagaaatgaa tattccgcgt tgctggaaaa tcctttttta tcatcctttt atgatagata
    26641 taaggatgat ttccacctgg atgtcagacg tgaatcatgt cggaaaattg aagccccttc
    26701 agagcccctt tcaagccaag aacaggaaca ggaacaggaa caggaaagag ataaaaccct
    26761 tctggtccat ggcgaaaaaa tcgccacgga cccactggtg gattcttgtc ccgttctgac
    26821 tgaacgtcca ggaccagctg gcatgacacc ggaagcagat tccgggcgtt gtgtgcagca
    26881 ggtgctgcac gtcgaaccgg agcaacaaca ccaaccgcag cagcctgaag ccgattccgc
    26941 gatgagcggg aagccgattg ggttaacccg ggcgatgccg ggaccgtctg cgggacgagt
    27001 tgattatcct gacgtgttcg aacgggtctg gcgtgaatat ccgcatcggg cagggtcaaa
    27061 cccgaagaaa tccgcgttca atgcctggag ggccagatta cgcgaagggg tgtcaccgga
    27121 tgtcgtgctg gatggcgtga ggcgttacgc aagatacctg gaggctaccg ggaaagcggg
    27181 aactgaattt gttcagcagg catcgacgtt ttttggcccg aacaggaatt tcgaaaatcc
    27241 gtggtcgctg ccgaaggctg gcgcagtcag cctgcgttgc gtgaatcaca tttctgaacc
    27301 ggacaccgaa attccgccgg gtttcagggg gtaatcagcc atgaaaaaca tttcgacagg
    27361 agggattctt gaacgggtgc gccgtctggt tccaccgcac gttacagcac cgttccggac
    27421 gaccgacgag tggctggaat ggcaactggc tgagggccgg aagcgcagcg aggaagttaa
    27481 ccgccagaat caccagacgc gggttgaaaa aatcctgaat cgggcatcca gccgcttcac
    27541 aggaagtgct cattcgggaa ctaccgggtg cagaacgacg gtcagcgcca tgctctgagt
    27601 caggcaaaat ccatcgcggc agagctgcat accggctgca cgaatttcgt gttcagcggt
    27661 aaacctggca ccgggaaaaa tcaccttgca gcagcgattg gcaactggct gatggcgaag
    27721 gggagaagcg tgattatcgt caccgtgtcc gatgtcatga gcgtgttgca tgacggctac
    27781 gacaacggca agtccgggga aaaattttta caggaacttt gcggggtaga ccttctggtc
    27841 cttgatgaaa ttggcatgca gcgggatacg cgcaacgagc aggtcatact gaaccagatc
    27901 gtcgaccgca gaacggcatc actgcgcagt gtcgggatgc tgacgaacct gaatcacgca
    27961 gctatgagca cactcctcgg cgagcgggtg atggaccgta tgaccatgaa cggtggtcgt
    28021 tgggtgaatt ttaactggga gagctggcgg tcaaacgttg ggcgtcaggg tatgtgagaa
    28081 tttttgacga ggtaaatttt cgatggaaac cgtattgcat gcactgaaag cgatgggtaa
    28141 agccaattct gttgaactgg cggcgcggct tgatatcagc cgtgaagaag ttctcaacga
    28201 actgtgggaa ctcaaaaaaa atggtgttgt tgataaaacg ggtcacacct ggtttctggc
    28261 tgtcgaaggt gaatccgggg taaccgaagg gcaggcacta caatctgaag cgccggatgt
    28321 ggtaaccgaa gaggtcgctc caaaagttag cgctgacatg atgattgagt ttatcgctca
    28381 ggagggggct aaaacctgtg aagaaatagc gggtaagttc ggagttacca ctcgtaaggt
    28441 tgcttccact ttggcggtag taacagcaac ggggcgcctg gtgcgcgtgt ctatcaaggg
    28501 aaaattccgt tataaagtgc ctgacgtcga aaacggcgat ttgagcgcgt catcatcgca
    28561 ggatgagaat atcacgcacg aagcagaaaa cagcgcgtca ggcgacgaac tgaatcgttt
    28621 tccgttgatg tcagagggca cggcaaaggc ctgtacagat gagtttatcc gggatgttcc
    28681 gtcgttcacc gaaaaatatg ctggtgcagt gacccttcca cctctacggg ccgccagtcg
    28741 tgaacttcgt cgcgccaaaa aacaggtcca gaagtgggag cgtgtctgtg ctgcactacg
    28801 tgagctgaat aaacaccggg aacttgtcat gcagattacc gattcatcca gcagtattgc
    28861 atcggaaaag tgataactgg agagcttatg gcaaaagtat ttacacccga acagcgggaa
    28921 gaactgaagg cacgtattgt ggagctcgtg cgcctgaatg gtcggggaac gttcaggcaa
    28981 ctggctactg aaaccggagt cagcaagaca gctgtcagac gtttgtccag agaactggtt
    29041 gtcagtggcg ctatattcga ctcaggttat ggtttattcc cgtctgatca ggcgcgtaag
    29101 gactggcaaa atgcccgcaa aaaactctcg agagcaaagg caaagaaacc ggttgttatt
    29161 gaccctggtc ttatccgggc attacctgat ggggaaatcc ggcgctacga cagacgctac
    29221 aacataattt gtcgcgagtg ccgtaacagc gaaacgatgc agcgtgtgct ggcattctgg
    29281 cgggggagtg cagagggatt gatgttctcc ccgtcgtgaa tgacgggggc ttacgtgttc
    29341 agaaaagtga ttcatataga ggctgaatct gatctttttt gttcacgtct ggctgccacc
    29401 attgcaggca gagtgaaggc gtagactaaa aacatttcgg taaaactcag gatctggctt
    29461 gcctcaattg gtgtgaaaac ttcatctgta tgaactgctt tattggcatc aattcgtaca
    29521 atatgagccc attccttcat ctgttcagtg atcagccctt ttttgtaaat catctgaata
    29581 cgttgcgaca gcgattcttt tcctgcttca tcgccgagca gttttttcgt ggagatatcg
    29641 aggactctgc gacaaagaat taccactgtg tcgtaccgcc tcctctgtaa atcctctttt
    29701 gcctcaacaa aagtacggtc tgcaaccggg ggcgtatatt ctggtgcggt aatttttttg
    29761 accgcaggat agatcttgcg gaaacggtac tggctatttc ctgaaatcag aatatcgagg
    29821 tcttttttct ggctttccgc catggggccg tgatagtggt ctgatgggat ttcaacaata
    29881 gcaacacctc ctcgatgaca acttctgcat acaaaagcga cgttaaaaag aggtgttttt
    29941 tcaatttgtt tttcggcaaa tgcctcaata acagctcgct cttttaaaca gtgtgggcat
    30001 gtaatgtcaa aggagactaa actcatggat attcctctcg aaattaaaga aattaacgaa
    30061 atcattgagg ttactgatcg ccccgagttt accctcatgc gtcgttatga aacaggaacc
    30121 gacgaacaaa aatacatcat ggttgcagca ttggctgtga tggcgataga aagggagcgg
    30181 agggaaaagg acgtaatggt tattccggcg agaaatgatt caccggattt gagatggcag
    30241 gatccggagt gggatgtggc aatccggtga ttgatatttc tctggggtaa agcgccgcca
    30301 gaatgacggc gcggtggtta agcatgaggc gaaatttctg ccgggcggta ttttgttgag
    30361 agatgagata cgtcaacttc tctttccgct ttttcaaggc tccatgccgt ctggagacga
    30421 atccatgaag acggagttcc acccaggaca gcagcgagac gaacagccat ttcaggagat
    30481 attgtcgcat tacctgaaac cagtcgctga acagtggaag gcgcgacatc aagcgctctt
    30541 gccagttccc tgattccaat attctgatct tccagaatat cagcaataat ttctccggga
    30601 tgaggggggt taaacatcgc cattagtggt aatcctcgta gttaagaata taagcatcgc
    30661 cgttccgaaa ctcgaatgtg attctccagt taccggagac ggtgacagac catgtatcgg
    30721 atctgtcacc ctgcaactgg tgcagcctgt acccggcaag gttaatatca tcaatgattt
    30781 cagcttgatc tattacctga aggcgcgttc tcaggcgttt aacgtgattg ggatttacgc
    30841 ctgatgcatt tcctgtttca aagaaacgtt gaagtccttt gtgtttccag ctttttatca
    30901 tgcttgtttc ctgttgcgtt acgcgaatca ttatagcgac tgttgtgtaa tgcgcaacgg
    30961 ttgtttgaca aaaataggta tccgggatta tattcttcgc acggtcgagt tgaccgtcgg
    31021 gattggaacc ccggatagag accgcgacaa acacacgccg cgagcgtgtt ttttattgtc
    31081 gtatgcacgc gcacatctga attatggtgg ggcgcatggg ggagctgaaa agctcgccgg
    31141 tcggtttccc ggtagttcca accctgtgcg tctcaccacc cgatgattgg aaccttacgg
    31201 tggtgatagt ttagaaacca ctagagggcg tcattatgac aactcaagtt tctgttgaaa
    31261 ctctttcccc gatcacccac aaccagatcc cagttattac aactgaactt cttgcccagc
    31321 tttacggcac agatgtaact aatattcgca aaaaccacag ccgaaaccat gaacgttttg
    31381 ttattggtaa acattatttt ttgctggaaa gtgcagaatt gcgtgagttt aagcacagag
    31441 tgtctttaag tcactctgtg acaatcgccc gtaacgttcg ctccctcatc ctctggacag
    31501 aacgcggcgc agcccgtcac gcaaaaatgc tcgaaaccga tcgggcttga cgttataggt
    31561 cgttacttgt agatttatct ttgtgaggcg tcagaacctc tctaaagcgg acaaaaccaa
    31621 ccccgtcagt gttggatttt ttatgcctgt tattcagtga tagcacaatg tgctgtcaca
    31681 tccccgaaca atgtcgggag ggcgacgaat acaacacccg taaggggaat aagtccgcgg
    31741 ttctttaggc cgttctgaac ctcccgacac cactatcaga ggtggtaatt tcagaaaaag
    31801 ctaaaggagg tcgttatgac catgcaactc gctgtagaat ccctcgtcgt cgttacttac
    31861 tctaacgtcc ctgtaataac aacagggtta ttggcaaatc tatacaaaac tgaagaaaaa
    31921 catatacggc agaacttcaa acgtaatgaa tgtcgtttca ttgcaggaaa acacttcttt
    31981 aaagtaagtg gctgtgaact tgatagtttg cgaacctctc aaagaggttt acaaatttcc
    32041 ccaaaaaccc gctccctcat cctctggaca gaacgcggcg ctgcccgtca tgcaaaaatg
    32101 ctcgaaaccg atcaggcgtg ggaagtgttc gaaaaactgg aagactgcta tttcagccag
    32161 ggaaaaacaa cacaaaccga acagcagtcg cagattcagc cacaattcac agccgaggaa
    32221 atcatcctcc tttgctacat gcagctctgg atggaaaaag cccaggacct cagcaaacac
    32281 ctgtatccca tcatgaaaga gctgaattca tcatacacga acaagctgta tgacattacg
    32341 tttgagacca tctacatggt gacgaagaac agagacgcgc tactgcggga ggcggcacgg
    32401 cttgaccagt caagtttcgt tgtccagcgg gccagaccaa tgctgaaaag cctgcgggca
    32461 agacaattcg agttttaagg ccagatttga caaatccgca ttagcgggga tatattccgc
    32521 tccatggtgc tgaacacacc ttcgaaagcg gaaaccgcgc ccgtcagtca tgcggatttt
    32581 ttatgtccat ttttcagata tggtcgggta gcgcgtatac cgaaaaacag ccgcaaggct
    32641 aaggatgcgg gccgaacttt cgacggtgtt caagtacccg accgccctgc tgaacacggg
    32701 gtgatctgaa cgaatcgaaa ggacataaaa ctatgagcac tcaactcgta ttcaaatccc
    32761 acgttcttga aacgatcgaa cacgacggca aacagtggtt cactgccgcg actttggctt
    32821 ctgcactgga gtattcgcgc ggcgataaaa tcacccagat atacaaccgc aattcggatg
    32881 agtttactcc ttgtatgtca atcaacctca aaatgaggtt caacgggatc aacaatagct
    32941 tacgagagaa agatgtgcgt attttctctc tacgcggtgc ccacctggta gcgatgttcg
    33001 caacaactcc agtagccaaa gagttccgtc gctgggtatt ggatattctg gaccgtgaag
    33061 ctgaaaaaag cgctataccg ccacaacctc atattcagcc gcaattcaca gcagaagaca
    33121 tcatcctgct ctgctacgtt caactctgga tggaaaaagc acaacaactc agtaaacagc
    33181 tataccccgt catgagagaa ctgaactctg attactacgg gaaactgttt gatctggcct
    33241 acgagactcg tcatataacc aaccggactc gcgacacatt gctccgcgaa gcagcaaaac
    33301 ttgatcccgc caactatatg gtgcataggg ccagaagcat gctggcgcaa ctacgggcaa
    33361 gacaatttga attctgaaac caaaggagct tcggctcctt ttttgttggg aaaatccagt
    33421 gagagggaat aatgaaccag actatcttcc tccgaagtaa gcagcagcaa caattcgcca
    33481 ttaacgcaat ccttgcaaca actctcgata aagacaaacc cgttacgatc cgcatcaccg
    33541 attacaagcg gaatctcgat caaaatgcca aatttcacgc gatggtcgct gatatctccc
    33601 gacaggttca gtggtgcggc agatggctaa aaccagaaca gtggaaagtt ttgttaatca
    33661 gcggtcatgc cgtggcgaca aaacaggaag ccgatgtttt acccggtctt gaaggcgaat
    33721 gcgtcaatat ccgcgaaagc agtgcgcaga tgagcgtgaa gcgtatggca agcctgattg
    33781 agtacacaac ttcctgggct gtggagaagg gggtcagatt taccgacagg aggtacgaat
    33841 gagacgacag cgacgaagca ttaccgatat agtctgtgaa aactgcaatt accttccaac
    33901 gaaacgctcc cgaaataaac ccaggccaat ccccaacgaa tctgatgtaa aaaccttcaa
    33961 ttatacgtct cacctgtggg atgtccggtg gctcagacgt cgtgcaagat ttaatagtca
    34021 ctccggatag ttcaatgtac gaggaataag atgatggcaa acctacgcaa agaagcgcgt
    34081 ggcagagaat gtcaggtacg gatttacggc gtatgcaatg gcaatcctga aactacagtt
    34141 ttggcgcatt accgaatagc tggaatttgc ggaacgggaa tgaagccaga tgacctgttg
    34201 gctgcatggg cctgtagtga ctgccataat gaaatcgatc gccgtactcg cattctcgac
    34261 aacaacgacg ccagacttta ccacctggaa ggcgtgatca ggacgcaggc gatactgctg
    34321 aaggagggga aaattaagtc atgaatgaat atgagtttgt gcttccctgg ccgccgacgg
    34381 tgaataccta ctggcgaaga cggggaagcc agtactacat cagcgataaa ggccagaaat
    34441 accgaaaaga cgtacagcaa atcatccggc aactcagatt agacattttc actaaatcac
    34501 gacttcgcat cacaattatt gctgaaccac cagattcccg ccgtcgcgac ctcgataaca
    34561 tcctgaaagg tttactcgac tctcttatct acgccggatt tgcggaagac gacgagcaat
    34621 tcgatgacat tcgcgtaatt cgcggcgtga aagtgcctgg cggtagagtg gggataaaaa
    34681 tcaccgaact ggagaacatt tgatgaatgc taaaattcaa acgatacctg aattactgat
    34741 ctgcaccagg ggaaatcaga cagaagtcgc cagaatactg aactgcaatc gtgccacagt
    34801 cagaaaatac attgatgata aagatgcgaa aaagcacgcc gtcgtcaatg gcgtccttat
    34861 ggttcatcgc ggatggggta aagatactga tgcgtgatat ccggcaggtt cttgagcgct
    34921 ggggtgcatg ggcggcaaat aactatgagg atgttacatg gtcgcccatt gctgcaggat
    34981 ttaagggact gatccccgaa aaagtaaaat cacgtccaca gtgttgtgac gatgacgcga
    35041 tgattatatg cgggtgtatg gctcgcctta acaggaacaa cagcgatctg catgacttgc
    35101 tggttgatta ttacgtgttg ggggagacat tcatggcgct ggcacgtaag catggatgct
    35161 ctgatggaac tatcggtaaa aaattacaga aagcggaggg tgtggttgaa gggatgttaa
    35221 tgttacttgg tgtcagactg gaaatggacc gatatgtgca acgcctctga caggaggcgt
    35281 ttgccgttca ctatgtgaag tgtgtcaatt tgtgagcgtt atgctgttta ctttatagtt
    35341 gcggaacgta agagttatcc tcctgctaag cgcagagatg caacaatgaa caggggggca
    35401 aaatggacat tgaagatttt ctggaggcag aagacaatga tctgtctctg tggaatgatg
    35461 atattttaag cattgactgg ttcgttattc ttagagagct gttgaaatat cagagaagac
    35521 taaatatcac ccattcagaa ttagttttgc tggctaattt ggtttcattt caccagaatg
    35581 tggattcgca aatattaccg tcaatatctc ttttggctac cagaatgcgc gcctcccgag
    35641 ccattataca aggacgcctt caggaactgg aggggaaagg aatgttgcag aagataagtt
    35701 acgctcaaat cggtcatgat gatgatttgc ggaacgtcta tgacatcaag ccattaattc
    35761 gtaagttaat tggggtgcag aaaaccgaga atgataaaaa gcacacttgc cccgtctgtg
    35821 gaaaagtagg ttcctctgat gaagaaattg agaaattttt tggattcaga aaaagtgggg
    35881 attataaatg gccgcaaagc tggtgccgtt catgcagaag cacgaaacaa cgccgactga
    35941 atacatcgct aattggtaaa aaaaggcccc gcaaatcccc accagaggtg gtgtgacgtg
    36001 tcgcggaatc cttgattccg ttcccgattg aatggggtgc taaaaagtat tttacgtacg
    36061 taaaaatctg catatcatga taagagtggt tgcattgcca cgcagccgac cccgtcgatg
    36121 agcggggttt ttttgtaccc agaatcctct gagctatacg gaaagtacac agaaaggaag
    36181 gtgcgaccac aattaataac aaaatctta
//
LOCUS       DAZGVQ010000033        36025 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000033 DAZGVQ010000000
VERSION     DAZGVQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.033"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(161..301)
                     /gene="yjjY"
                     /locus_tag="ACHLAG_001605"
     CDS             complement(161..301)
                     /gene="yjjY"
                     /locus_tag="ACHLAG_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFP0654856.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            397..1113
                     /gene="arcA"
                     /locus_tag="ACHLAG_001606"
     CDS             397..1113
                     /gene="arcA"
                     /locus_tag="ACHLAG_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFP0654857.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1172..2524)
                     /gene="creD"
                     /locus_tag="ACHLAG_001607"
     CDS             complement(1172..2524)
                     /gene="creD"
                     /locus_tag="ACHLAG_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFP0654858.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTAQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDEPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQCQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTGAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(2582..4006)
                     /gene="creC"
                     /locus_tag="ACHLAG_001608"
     CDS             complement(2582..4006)
                     /gene="creC"
                     /locus_tag="ACHLAG_001608"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFP0654859.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(4006..4695)
                     /gene="creB"
                     /locus_tag="ACHLAG_001609"
     CDS             complement(4006..4695)
                     /gene="creB"
                     /locus_tag="ACHLAG_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFP0654860.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4708..5181)
                     /gene="creA"
                     /locus_tag="ACHLAG_001610"
     CDS             complement(4708..5181)
                     /gene="creA"
                     /locus_tag="ACHLAG_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFP0654861.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSYRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5392..6261
                     /gene="robA"
                     /locus_tag="ACHLAG_001611"
     CDS             5392..6261
                     /gene="robA"
                     /locus_tag="ACHLAG_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFP0654862.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6258..6905)
                     /gene="gpmB"
                     /locus_tag="ACHLAG_001612"
     CDS             complement(6258..6905)
                     /gene="gpmB"
                     /locus_tag="ACHLAG_001612"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFP0654863.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6957..7478
                     /gene="yjjX"
                     /locus_tag="ACHLAG_001613"
     CDS             6957..7478
                     /gene="yjjX"
                     /locus_tag="ACHLAG_001613"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFP0654864.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(7563..7889)
                     /gene="trpR"
                     /locus_tag="ACHLAG_001614"
     CDS             complement(7563..7889)
                     /gene="trpR"
                     /locus_tag="ACHLAG_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFP0654865.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(7979..9916)
                     /gene="sltY"
                     /locus_tag="ACHLAG_001615"
     CDS             complement(7979..9916)
                     /gene="sltY"
                     /locus_tag="ACHLAG_001615"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFP0654866.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTAAHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10127..11794
                     /gene="ettA"
                     /locus_tag="ACHLAG_001616"
     CDS             10127..11794
                     /gene="ettA"
                     /locus_tag="ACHLAG_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFP0654867.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVE
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(12101..13333)
                     /gene="nadR"
                     /locus_tag="ACHLAG_001617"
     CDS             complement(12101..13333)
                     /gene="nadR"
                     /locus_tag="ACHLAG_001617"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFP0654868.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13354..14736)
                     /gene="radA"
                     /locus_tag="ACHLAG_001618"
     CDS             complement(13354..14736)
                     /gene="radA"
                     /locus_tag="ACHLAG_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFP0654869.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14785..15753)
                     /gene="serB"
                     /locus_tag="ACHLAG_001619"
     CDS             complement(14785..15753)
                     /gene="serB"
                     /locus_tag="ACHLAG_001619"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFP0654870.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15859..16503
                     /locus_tag="ACHLAG_001620"
     CDS             15859..16503
                     /locus_tag="ACHLAG_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFP0654871.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16531..17547
                     /gene="lplA"
                     /locus_tag="ACHLAG_001621"
     CDS             16531..17547
                     /gene="lplA"
                     /locus_tag="ACHLAG_001621"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFP0654872.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSTWIA
                     GAVR"
     gene            17579..17842
                     /locus_tag="ACHLAG_001622"
     CDS             17579..17842
                     /locus_tag="ACHLAG_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0654873.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(18003..18722)
                     /gene="deoD"
                     /locus_tag="ACHLAG_001623"
     CDS             complement(18003..18722)
                     /gene="deoD"
                     /locus_tag="ACHLAG_001623"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP0654874.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(18802..20025)
                     /gene="deoB"
                     /locus_tag="ACHLAG_001624"
     CDS             complement(18802..20025)
                     /gene="deoB"
                     /locus_tag="ACHLAG_001624"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP0654875.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(20077..21399)
                     /gene="deoA"
                     /locus_tag="ACHLAG_001625"
     CDS             complement(20077..21399)
                     /gene="deoA"
                     /locus_tag="ACHLAG_001625"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFP0654876.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQDAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(21526..22305)
                     /gene="deoC"
                     /locus_tag="ACHLAG_001626"
     CDS             complement(21526..22305)
                     /gene="deoC"
                     /locus_tag="ACHLAG_001626"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFP0654877.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22563..24113
                     /locus_tag="ACHLAG_001627"
     CDS             22563..24113
                     /locus_tag="ACHLAG_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFP0654878.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            24085..24948
                     /locus_tag="ACHLAG_001628"
     CDS             24085..24948
                     /locus_tag="ACHLAG_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFP0654879.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(25263..26045)
                     /locus_tag="ACHLAG_001629"
     CDS             complement(25263..26045)
                     /locus_tag="ACHLAG_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0654880.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(26042..27115)
                     /locus_tag="ACHLAG_001630"
     CDS             complement(26042..27115)
                     /locus_tag="ACHLAG_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFP0654881.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(27237..27398)
                     /locus_tag="ACHLAG_001631"
     CDS             complement(27237..27398)
                     /locus_tag="ACHLAG_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFP0654882.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(27525..28130)
                     /gene="osmY"
                     /locus_tag="ACHLAG_001632"
     CDS             complement(27525..28130)
                     /gene="osmY"
                     /locus_tag="ACHLAG_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFP0654883.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(28523..30112)
                     /gene="prfC"
                     /locus_tag="ACHLAG_001633"
     CDS             complement(28523..30112)
                     /gene="prfC"
                     /locus_tag="ACHLAG_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFP0654884.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVQASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTLGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(30203..30880)
                     /gene="yjjG"
                     /locus_tag="ACHLAG_001634"
     CDS             complement(30203..30880)
                     /gene="yjjG"
                     /locus_tag="ACHLAG_001634"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFP0654885.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(30895..31341)
                     /gene="rimI"
                     /locus_tag="ACHLAG_001635"
     CDS             complement(30895..31341)
                     /gene="rimI"
                     /locus_tag="ACHLAG_001635"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP0654886.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(31310..31723)
                     /gene="holD"
                     /locus_tag="ACHLAG_001636"
     CDS             complement(31310..31723)
                     /gene="holD"
                     /locus_tag="ACHLAG_001636"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFP0654887.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            31826..32857
                     /gene="rsmC"
                     /locus_tag="ACHLAG_001637"
     CDS             31826..32857
                     /gene="rsmC"
                     /locus_tag="ACHLAG_001637"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFP0654888.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            33125..33211
                     /locus_tag="ACHLAG_001638"
     tRNA            33125..33211
                     /locus_tag="ACHLAG_001638"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33159..33161,aa:Leu,seq:cag)
     gene            complement(33250..33486)
                     /locus_tag="ACHLAG_001639"
     CDS             complement(33250..33486)
                     /locus_tag="ACHLAG_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFP0654889.1"
                     /translation="MLQRMLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            33627..34415
                     /gene="fhuF"
                     /locus_tag="ACHLAG_001640"
     CDS             33627..34415
                     /gene="fhuF"
                     /locus_tag="ACHLAG_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFP0654890.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(34453..35130)
                     /gene="bglJ"
                     /locus_tag="ACHLAG_001641"
     CDS             complement(34453..35130)
                     /gene="bglJ"
                     /locus_tag="ACHLAG_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFP0654891.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(35088..35813)
                     /locus_tag="ACHLAG_001642"
     CDS             complement(35088..35813)
                     /locus_tag="ACHLAG_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0654892.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
BASE COUNT         8582 a         9831 c         9083 g         8529 t
ORIGIN      
        1 ttattagttg tatgatgcaa ctagttggat tattaaaata atgtgacgaa agctagcatt
       61 tagatacgat gatttcatca aactgttaac gtgctacaat tgaacttgat atatgtcaac
      121 gaagcgtagt tttattgggt gtccggcccc tcttagcctg ttatgttgct gttaaaatgg
      181 ttaggatgac agccgttttt gacactgtcg ggtcctgagg gaaagtaccc acgaccaagc
      241 taatgatgtt gttgacgttg atggaaagtg catcaagaac gcaattacgt actttagtca
      301 tgttacgccg atcatgttaa tttgcagcat gcatcaggca ggtcagggac ttttgtactt
      361 cctgtttcga tttagttggc aatttaggta gcaaacatgc agaccccgca cattcttatc
      421 gttgaagacg agttggtaac acgcaacacg ttgaaaagta ttttcgaagc ggaaggctat
      481 gatgttttcg aagcgacaga tggcgcggaa atgcatcaga tcctctctga atatgacatc
      541 aacctggtga tcatggatat caatctgccg ggtaagaacg gtcttctgtt agcgcgtgaa
      601 ctgcgcgagc aggcgaatgt tgcgttgatg ttcctgactg gccgtgacaa cgaagtcgat
      661 aaaattctcg gcctcgaaat cggtgcagat gactacatca ccaaaccgtt caacccgcgt
      721 gaactgacga ttcgtgcacg caacctgctg tcccgtacca tgaatctggg tactgtcagc
      781 gaagaacgtc gtagcgttga aagctacaag ttcaatggtt gggaactgga catcaacagc
      841 cgttcgttga tcggccctga tggcgagcag tacaagctgc cgcgcagcga gttccgcgcc
      901 atgcttcact tctgtgaaaa cccaggcaaa attcagtctc gtgctgaact gctgaagaaa
      961 atgaccggcc gtgagctgaa accgcacgac cgtactgtag acgtgacgat ccgccgtatt
     1021 cgtaaacatt tcgaatctac gccggatacg ccggaaatca tcgccaccat tcacggtgaa
     1081 ggttatcgct tctgcggtga tctggaagat taatcggctt taccaccgtc aaaaaaacgg
     1141 cgctttttag cgccgttttt atttttcaac cttatttcca gatacgtaat tcatcgtccg
     1201 ttgtaacttc tttactggct ttcattttcg gcagtgaaaa cgcataccag tcgatattac
     1261 gggtcacaaa catcatgccg gccagcgcca ccaccagcac actggttccc aacaacagcg
     1321 cgctatcggc agagttgagc agtccccaca tcacaccatc cagcaacaac agcgcgaggg
     1381 taaacaacat gctgttgcgc cagcctttca ataccgcctg caaataaata ccgttcatta
     1441 acgccccaat cagactggcg attatccatg ccccggtaaa accggtatgt tcagaaagcg
     1501 ccagcaagag caaataaaac atcactaatg aaagccccac cagtaaatat tgcattgggt
     1561 gtaaacgttg cgcggtgagc gtttcaaaaa caaagaacgc cataaaagtc agcgcaatca
     1621 gcagaatagc gtacttagtc gcccggtcag ttaattggca ttgatcggct ggcgtcgtca
     1681 ctgcgacgct aaacgcgggg aagttttccc agccggtatc attgcctgaa gcaaaacgct
     1741 caccgagatt attagcaaac cagctgcttt gccattgcgc ctgaaaacct gactcactaa
     1801 cttcccgttt ggctggcaaa aaatcaccta aaaaactggg atgcggccag ttgctggtta
     1861 aggtcatttc gctattacgc ccggcaggca ccacagaaag atcgccggta ccgcttaaat
     1921 tcagggccat attcagcttc aggttctgct tgcgccagtc cccttcaggt aaagggatat
     1981 gcacgccctg cccgccttgc tctaacccgg tgccgggttc aatggtcagc gccgttccgt
     2041 taacttcagg cgctttcacc acaccaatac cacgcgcatc cccgacgcta atcacaataa
     2101 atggcttgcc taaggtgata tttggctcgt cgagttcgct aagacgcgaa acatcgaaat
     2161 cggcttttag cgttaaatca ctgtgccaga cctgaccggt ataaatccct atcttgcgtt
     2221 cttcaacgtt ctgattgcca tcaaccatca atgactcagg taaccaaaaa tggataaaac
     2281 ttcgtttccg ctccacggtt ttatcgtctt cctgcgccgt ataaagctcg gtcacaggaa
     2341 tagcgatgag cggcccaacg agtttttgcg gcccgctggt actttgccga atcgcatctt
     2401 ccacatcgct acggtaatcg gcacgttcga caatcacttg ccgaatcagc attatcggaa
     2461 tcaacaacag caatactgca ccaaacaagg tggtcatttt ccagaacagg ggggatttca
     2521 acatagtctt ctcctttgca atggcagcag gatacgaagg ctatgtggga agaatttgaa
     2581 gctatgtgaa gtgacggtga agtcgaagcg aggccagcac gccaccttcc tgcacgttgc
     2641 gcagcgtgac ttcgccgtta aacaaacggg cgacctcact gacgaacgcc aaccccagac
     2701 cgctgctttt ttgcccattt gcacgcggca aagagtaaaa gcgttcaaaa atacgtgaca
     2761 gcgcgtagtc aggaatacca ctaccggtat ccagcacctt aagggcgacg tgttcctgat
     2821 ccacttcggc gcttagcgtt atgcgaccgc tctcgggggt aaaatcgatg gcgttatcca
     2881 gtaaattccc cagcgcctgc tccagtaacg ccggttcagc agcaacatta acctcagtgg
     2941 gcataacatg tagagtgatg tttttttctg ccaactgcac ggtgcgcgct tcgctgacgc
     3001 ggcgaaataa tgccgccaca tcaacaacag tcagaacgac ttcctgacga ttctccagtc
     3061 ttgcctggcg tagtaacgtt tctaccaatg cctgcatacg cgcattttgc gtcagaatgt
     3121 tgtcggtaaa acgagccacc acttccggtg gcggaccttc gcgtaaaatt tccgccgcgc
     3181 cacgaatcgc cgccagtggg ctttttagct catgggttaa cgcataaaca tactgctcaa
     3241 tatagttttt cccttccagc ttcacgcgca tactttccag cgcctgcgca agcttacgca
     3301 actcgctact gccgagttca ggaagaggaa cgggcttatt gtcagtgacg gaatcggcat
     3361 agcgagtgag ccgggcaatg gagcggttga tccaccaaac catgcctgcg ccaatcacca
     3421 gtgcaatccc cagcaaaatg gcgctggccc ataaaattcg ccgctcgcta cgcttaatga
     3481 ccggagccat cgccgcgttc ggtttgccta cgctcaaaac gccaataagc cgcgagccgt
     3541 ccataatcgg cgcggcaaca tacatcaccg aactttcggg atcggcagga ttttgcaacg
     3601 tgctgcgcgc accatactga ccacgcaacg ttagccagac gtcattccag cgcgaataat
     3661 cctgcccaac ggctttattt gccgaatcga acaatacttt accctgcgaa tcggtcatat
     3721 agacatgata ttcattgcgc actttgttaa tgccaccgat attggcgcga aacgggcgat
     3781 gttgtagctg attaaacgcc tgcgccagtt gcccatgcgt tgggtcccca gagagcaaat
     3841 cgggacgcgc cagctccgcc agcaacgttg cggtatcgat taacgtcccc tccgttgctc
     3901 ttcgcacgcc cggtttaact tctttgacaa aaatagccag tacgaaccag gccgccaccg
     3961 ccaccagtaa aaaatagccc agcagcaacc gcatgccgat acgcattaca ggcccctcag
     4021 gctatatccc atgccgcgat gagtattaat cggtgaaaga tcggggttga tggcgcgcag
     4081 cttggcacgc aacgttttaa tgtgggtatc aacggtgcga tcgtaggtgt cctgcgcatc
     4141 ttcccacacg ctatccatca gttgctggcg ggaccagacg cggcctggcg acttgagtaa
     4201 cgtcttcagc aataaaaact cataccgcgt cagcgttaat ggcgtgtcaa accagctgat
     4261 ctgcgccgcg ggttcattca attcaaaatg accaatacgg atgacgggag acggcgacga
     4321 gaacttcttc acccgacgca gtaaggtgcg caccctggcg cacacttcgc ggggtgaaaa
     4381 cggtttagcc acatagtcgt cagcgccaat ttccagccca agcaggcgat cgacctcttc
     4441 acttcgggcc gtcaggaaca gtacaggtaa cgccggatgg agcgccagta actggcggca
     4501 taattcaaag ccgctgatat caggcaaacc aacatcgaga atcatgacgt cgggaacctg
     4561 ctggcgagct ttatccagca ccggcaagcc tcgctcaaag acctcgacgg caaaaccttc
     4621 ctgctgcaac atgtagacta gcgtgtcggc tatcccttgc tcatcttcca ctaaccagac
     4681 cgtttcccgt tgcataaatc gcctctgtta ttgccgccac ggcatgacag gaaccgcgct
     4741 aatcgcgttt ttcggcgaac cttctacaac tttgtcggag taagccagat aagcgagcgc
     4801 gttgcgtttg gcgtcataaa agcgcacgac ctgtaacgac ttaaagacca gggacgtgcg
     4861 ttttttgaat actacctcgc cctgagcttt gccgttttta atacgatacg acagttcaat
     4921 cggcccgact tgctgacaag aaatagccgc atcggaggta tcttccgcca gacccaatcc
     4981 ccctttaata ccacctgttt tcgcccggct cacataacag gtgacatttt tcacatcggg
     5041 atcgtcaaag gcttccacaa caattttgtg atccgggccg atcattttaa atacggtgtc
     5101 gaccgaacca atctcttctg catgagccaa tggccccagc attatcaggc ttaaagacag
     5161 gatcaaatgc ttgtatttca tattgttacc atttttatct attacacgag caattagttc
     5221 gtcacggtaa ataacatatt gattaagaac aaaatctcaa tacttttatt tccgtcaagc
     5281 cctaaaacat actctactaa gaaaaaaaca ctgaatgcta aaacagcaaa aaatgctatt
     5341 atccaattac ctgatgtcag gtgctcgttg ttgaaaggat gaggatattt tatggatcag
     5401 gccggcatta ttcgcgacct tttaatctgg ctggaaggtc atctggatca gcccctgtcg
     5461 ctcgacaatg tagcggcgaa agcaggttat tccaagtggc acttacagag aatgtttaaa
     5521 gatgtcactg gccatgctat tggcgcgtat attcgtgctc gtcgtttgtc gaaatcggcg
     5581 gtcgcactac gcctgactgc gcgtccgatt ctggacatcg cgctgcaata ccgcttcgac
     5641 tctcaacaga catttacccg cgcattcaag aagcagtttg cccagactcc tgcactttac
     5701 cgccgttctc ctgaatggag cgcctttggt attcgcccgc cgctacgtct gggtgaattc
     5761 acaatgccag agcacaaatt tgtcaccctg gaagatacgc cgctgattgg tgttacccag
     5821 agctactcct gttcgctgga gcaaatctct gatttccgcc atgaaatgcg ttatcagttc
     5881 tggcacgatt ttctcggcaa cgcgccgacc attccgccag tgctctacgg cctgaatgaa
     5941 acgcgtccga gtcaggataa agacgacgaa caagaggtat tctataccac tgcgttagcc
     6001 caggatcagg cagatggcta tgtactgacg gggcatccgg tgatgctgca gggcggcgaa
     6061 tatgtgatgt ttacctatga aggtctggga accggcgtgc aggagtttat cctgacagta
     6121 tacggaacgt gcatgccaat gctcaatctg acgcgccgta aaggtcagga tattgagcga
     6181 tactacccgg cagaagatgc caaagcggga gatcgcccaa ttaatctgcg ctgtgaactg
     6241 ctgattccga tccgtcgtta acgctgcaac tcatctaatg caggggcgtc cagatgcgag
     6301 atgtcccctg cggtttcaac aacccagcca gacgccagcc acaggctttc ctgataatcc
     6361 acgcgcgaaa tagaacagtt acgcagacgt aagcggcgct ctgcccatgc tggtaatccg
     6421 agaatcgtac tcaccagaca tcccagtgca ataccatggc tcaccagcag cggtcggctt
     6481 ccctgcggta agtcccggca agattccagt gccgcattga cgcgatcgct gagctcctgc
     6541 attgactcgc cttcaggaat acgcccgtca acggtgccat tgaccagctg ccgacgccag
     6601 ttctcttctt cttcggtcag agaatcgata tgtctttttt ccagcacacc catgtttaat
     6661 tcacgcaggc gagaatcaaa gatgatgtca cagccgcagg cctgggcgat gatttccgcc
     6721 gtacgccggg tgcgtcctaa atcgctactg ataatatgcg taatgccaag ctctttggca
     6781 cgggttgcca cctgcatcgc ctgttgctca cctttggcgg tcagcgggct gtcagactgg
     6841 ccctgaatac gtcgctcggc gttccactgc gtttcaccgt ggcggactag gtatacctgt
     6901 aacatgcttt ttttccgtat actgctgtaa cgttaatttt ctgagtaatg ctgattatgc
     6961 accaagttgt ctgtgcgacc accaatcccg ctaaaattca ggccattctg caggcatttc
     7021 acgagatctt cggcgaagga tcctgccata ttgcatccgt tgccgtcgag agcggtgtac
     7081 cggaacagcc ctttggcagt gaggaaacgc gcgctggcgc acgaaatcgg gtagcaaatg
     7141 cccgccgttt acttccagag gctgattttt gggtggcgat tgaagctggc atcgatggtg
     7201 acagcacttt cagttgggtg gtgattgaaa acaccagcca gcgcggcgag gcgcgttcgg
     7261 ctactttgcc gttacctgcg gtgattctgc agaaagtgcg tgaaggtgag gcgcttggtc
     7321 ccgtgatgtc gcgttatacc ggtattgatg agattggtcg taaggaaggg gcgatcggcg
     7381 tgtttaccgc cgggaaactc actcgcgcca gcgtctatca ccaggcggtg atcctggcgc
     7441 tgagtccgtt tcacaatgcc gtgtatcagg cattgtagga cggataaggc gttcacgccg
     7501 catccggcat tcggtgcacg atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa
     7561 aatcaatcgc ttttcagcaa cacctcttcc agccactggc gcagctccac gggcgcggct
     7621 ttcaggctgt tagatccacg cgtaatcgtc gcgatgcccg cgccgagttc attttttaac
     7681 tcacgctggc tcatttcgcc gcgcaacagc tcttcgacaa tacgcacccg agtccccaac
     7741 gcttcgcgct catctggcgt cagcatcaga tttaacaacg gtaaatggag atcgttttgg
     7801 taggcattct taagcaggtc gacaaaacgt aaccactcct ggtgacgctg ttctgccatc
     7861 gctgctgaat agggtgattg ttgggccata atatgtcgcc attgttagcg ggggaagcaa
     7921 aatgcctccc ccggttgtac tcgctaaaga gtacgatagc atatcataaa cgtgcggatc
     7981 agtaacgacg tccccattcc gtggcgctca tcaacgtcgg tttatccccc atgaaatagc
     8041 ggtagtaagc gtcataagcc agcacgttct tcacataacc gcgcgtctcg gagaacggaa
     8101 tactctcgac aaatgccaca gcgtcgatac gcccggcgct gttgccaagc caggttcgca
     8161 cccgtccagg tccggcgtta taagctgctg aggagaaaat acggttattg ccaaactgct
     8221 gataaacata ttgcaggtaa ctggtgccaa tgttgatatt cgtttccgga tccagcaatt
     8281 gcccagggct gctataacca ggaatagaga acatcttcac cgtatgggcc gctgtaccag
     8341 gcataatctg catcaggccg ctggccccta ccggtgattt cactttcgga ttccaggcgc
     8401 tctcctgacg ggcaatcgcc atcgcatagc tttgcgggat ctctttccca ctggtgtaac
     8461 gtttgaaaag atcgttgtaa gccagcggga atcgctcttc cagatgatcc caaagcttcc
     8521 cggcgatcgt tgcctgaacg ctaagatccc accattggtt attgaaagca taccgagcca
     8581 gttgagcctg ctctgttttt gacttgctct tcaccagatt ggcccactcg ctacgcgcgg
     8641 tgttatcgag attccagtac atcaactcgc gcacgcgcgc catctccggc ccctgagtca
     8701 gggcgctgtc aacattctgc ggcgctttat caatcttcag ctcatactct tctccgatgc
     8761 gttgtgctgc aaccatcggg tagaaaccac gctgttgcat gagttgatgc aaaatctctt
     8821 tcgcttcagc ttcacgtccg cgttccagca ataaatccgc ctgccagtaa cgccattcat
     8881 ctttctcttt tgcttccatc ggcagacgcg ccagccaggt attcaggccg cgacgatcgc
     8941 cggtgccaag tgccattcgc acacggcgtt caatcaacga cgtggattgc gagcgcataa
     9001 tggcgtcatc gcgccatttc gcctgctcgt cggtgacatc gttgcccatc aaacgccagg
     9061 cgacgatatc gcgcagctcc tgaatctgat cttcattaag ctgctgcgcc tgggcaagcg
     9121 acgggatcat cagccgtgca ttttcagcat cctgccgcgc cacactggca aacgccaccg
     9181 ccgccatttg acgggtaaaa tcggtcgctc cggttgtacg cgcgaaggtc agtaccgtat
     9241 tagggttgtt cgccagtgaa atgattgccg aggcgatagt ctggtaatcg gcaggcatct
     9301 gccctgccag cactgttacc aggccagtgt tacccgcttt catcgccaga cggatacgct
     9361 ctaaatacgc cagcggatct tgtttacctg acgcacgcca gacgctaaat aacttgtcac
     9421 aggcgttagg ctggctcttg ccggttagcc acagctcttt cgccccttgc caggcttctt
     9481 cactctgccc ggtgttccat ttcgcatagt agtaattaca ttgcgcttcg gtagttccgg
     9541 gcttttccgg gctaaaggct aacaagccac gccagtcttc acgccgcgcc agttcattga
     9601 cgaaacgaga ttgcagcgtg cgagcgggag gaagcgtggg gttagcgcga acaaagttag
     9661 tgaccgtcac cgccggttga ttcatcagat catcggtgat ctggcggtat tccaggtagg
     9721 gataaagcgg ataatccttc agtccaggca tcatttgttc gaccacatcc atttgtcgat
     9781 tatcccaggc ctgcttgatt tgcgcgtaac ggctacgctg ctcatccagt gagtcggctc
     9841 gcgccacgct gctgaccgtc agcagacaga caccggcagc caacagccgc caggtaactt
     9901 gtttggcttt ttccacaagc gcatcctcta agtgtaaata catcaatgca gcgtcatgcc
     9961 gcgtaataga tttatgctaa ccagtcattg ccgtttacgc cacgttacgg acactttttt
    10021 acttttactg cgaaggtgat cgaccaactg ggattaggcg gggaaaaagg ctacacttcg
    10081 cctttgaaaa ctcatcatca cgataaacat agaggcgaag tccaacgtgg ctcaattcgt
    10141 ttataccatg catcgtgtcg gcaaagttgt tccgccgaaa cgtcatattt tgaaaaacat
    10201 ctctctgagt ttcttccctg gggcaaaaat tggtgtcctg ggtctgaacg gcgcgggtaa
    10261 gtctaccctg ctgcgcatta tggcgggcat tgataaagac atcgaaggtg aagcgcgtcc
    10321 gcagccagac atcaagattg gttacctgcc gcaggaaccg caactgaacc cggaacacac
    10381 cgtgcgtgag tccattgaag aagcggtttc tgaagtggtt aacgccctga aacgcctgga
    10441 tgaagtgtat gcgctgtacg ccgatccgga tgccgatttt gacaagctgg ccgctgaaca
    10501 aggccgtctg gaagagatca ttcaggctca cgacggtcat aacctgaacg tacagctgga
    10561 gcgtgcggcg gatgcgctgc gtctgccaga ttgggacgcg aaaatcgcta acctctccgg
    10621 tggtgagcgt cgtcgcgtgg cgttgtgccg cctgcttctg gaaaaaccag acatgctgct
    10681 gctcgacgaa ccgaccaacc acctggatgc cgaatccgtg gcctggctgg aacgcttcct
    10741 gcacgacttc gaaggcaccg ttgtggcgat tactcacgac cgttacttcc tcgataacgt
    10801 tgcaggctgg atcctcgaac ttgaccgcgg tgaaggtatt ccgtgggaag gcaactactc
    10861 ctcctggctg gagcagaaag atcagcgcct ggcgcaggaa gcttcacaag aagcggcgcg
    10921 tcgtaagtcg atcgagaaag agctggagtg ggtacgtcag ggaactaaag gccgccagtc
    10981 gaaaggtaaa gcacgtctgg cacgctttga agagctgaac agcaccgaat atcagaaacg
    11041 taacgaaacc aacgaactgt ttattccacc tggaccgcgt ctgggcgata aagtgctgga
    11101 agtcagcaac ctgcgtaaat cctacggtga tcgcctgctg attgatgacc tgagcttctc
    11161 gatcccgaaa ggggcaatcg tcgggatcat cggtccgaac ggcgcgggta aatcgaccct
    11221 gttccgtatg atctctggtc aggaacagcc ggacagcggc accatcactt taggtgaaac
    11281 ggtgaaactg gcatcggttg agcagttccg tgactcaatg gataacagca aaaccgtttg
    11341 ggaagaagtt tccggcgggc tggatattat gaagatcggc aacaccgaga tgccaagccg
    11401 cgcctacgtt ggccgcttta actttaaagg ggttgatcag ggtaaacgcg ttggtgaact
    11461 ttccggtggt gagcgcggtc gtctgcatct ggcgaagctg ctgcaggttg gcggcaacat
    11521 gctgctgctc gacgaaccaa ccaacgacct ggatatcgaa accctgcgcg cgctggaaaa
    11581 cgccctgctg gagttcccgg gctgtgcgat ggttatctcg cacgaccgtt ggttcctcga
    11641 ccgtatcgcc acgcacatcc tggactacca ggatgaaggt aaagttgagt tcttcgaagg
    11701 taactttact gagtacgaag agtacaagaa acgtacgctg ggcgcagacg cactggagcc
    11761 gaagcgtatc aagtacaagc gtattgcgaa gtaatgcgta aaatgccgga tgcggcgtga
    11821 acgccttatc cggcctacaa accaagaaat ttcaatacgt tgtgaatctc caggcctgat
    11881 aagacgcggt aagcgtcgca tcaggcattg agcaccgaat gccggatacg gcgtgaacgc
    11941 cttttccggc ctacaacagc cttagtatcc gcaaattcgt agcgggtcac ccgtgctgat
    12001 tcttttaaac cttgtttaat ctcgacagcc tcttccagag aggttttcaa ctcatcaaaa
    12061 aacttatttt atacctcgcc tttgagccgt ttcatcgcgg ttatctctgc tcccccatca
    12121 tctcccgcac cagctccacg cagcgcagga aacggctgtc ataatcgtcc tcttcaaccc
    12181 gcacgaattc gatattgttc tcttccagca tctccaccag caagttctgg aactctttgc
    12241 gatccaccga actgccgagg ctgcgtaaac catccgccac ccacggcgtg ttgttctcca
    12301 gcaggatcac cagatcgaaa cggtattcat caatcaacgc ctgtacgaac ggatgctcac
    12361 gcccttcgta ctttttgcag aacgcctggg tagtgacaaa atcagtgtca ataaacgcca
    12421 ctttattggc atatttcact gcaaaatcaa tgtattgtgc gtggcccagc gcgattttat
    12481 cgtaatcgga atactgcaat gcgatctcat caccgccgag gtgtgaaaag acataatcgc
    12541 gaccatattc ccacgcactg gtggtattga agatattggc aagtttgttt accaaagtgg
    12601 atttaccgct cgactcgcca ccaaggatcg ccacggtacg tacaaagaac ggcttcactt
    12661 ccgtaggaat atattcccag tagcggaacg ggttttcgcg gatctgcgca ccgctgatac
    12721 tcataaaggt acgtttcgga tcgaccagca ccgtatcgat ccccagatgt tccatatact
    12781 gtggcgcatc ggcttcttcc gaggtgtaga tcaggtccgg ctgaatcccc ttttcagcca
    12841 taaacttttt gatgccgttg ctccacacat cccagccgtg cggatacggc tccatgccct
    12901 cttcgttgaa agcatgaatg cgaatatttt tctgatattt aaaagtttgt aataaccagc
    12961 gcagacgatc cggcaccgtg ggctgctgcg acatggcgct gtcttcaaac aacgcgcggt
    13021 cgcgggtatc gtcaaaaccc ataatgatat gcagctcgtc aacctggcta caggcgcgct
    13081 ggataaggta gatatgtccg gtatgcagcg ggtagaactt accgaatacg acaccaatgg
    13141 ttttcttctg tcggggaaat tcaagcccca aaaaacggtg caacgcctcc agcttttgcg
    13201 cgctggggct tttaattttg gcattcagta actggcttaa ataccctttg gtcataccgc
    13261 tggcatcagc tacctgctgt agcgtgcagc cctgttgctt gatggcagtt ttcaggtaat
    13321 caaatgacga catatctccc tccgtatctc tcattataag tcgtcgaaca cgctaagcgc
    13381 gtcggagagt tttttaacgc caaaaatctg catcccttcc ggcgcttttt tcggtacgtt
    13441 agccgccgga acaatcgccc ggcgaaaacc gtgtttcgcc gcttctgaaa ttcgttcctg
    13501 accgctgggc accgggcgga tctcccctgc cagcccgact tcaccaaaca ccaccagatc
    13561 ctgtggcagc ggtctgtcac gcaggctgga aaccatcgcc agcagtaacg ctaaatcggc
    13621 actggtttcg gttaccttca cgccgccgac cacgttcaca aatacatcct gatcggccat
    13681 ttgcagacca ccgtgacggt gcaagacagc cagcaggatt gccagacggt tttgttccag
    13741 ccccactgcc acgcggcgcg gattcgccat catcgagtga tcgaccagcg cctgaatctc
    13801 caccagcaac ggacgcgttc cttcccacac caccatcacg gagctgccgg aggtcacttc
    13861 atctccgcga cttaagaaaa ttgccgaagg gttgctgact tcacgcagcc cctgttcggt
    13921 catcgcgaag acgcccagct cattcaccgc gccgaagcgg tttttatggc tgcgcaaggt
    13981 gcgaaaacgg gagtcggcat caccatccag aagcaccgaa cagtcgatac agtgttccag
    14041 cactttaggg ccagccagcg agccatcttt ggttacgtgc ccaaccatga caatcgccac
    14101 accgcgcgtt ttggcgaagc gcgtcagata agccgccgtt tcacgcacct gcgccacgct
    14161 accaggcgat gattgtacat ccgccatatg catcacctgg atcgagtcaa ttaccatcag
    14221 tttcggttgc tcttcttcgg caatcaggca gatctgttcg atgctggttt ccgacaacat
    14281 attgagattg tcagtcggca ggccaaggcg atgagcgcgc attgccacct gttgcagcga
    14341 ctcttcgcca gtgacataca gcgttttcat ctgctgggcc agtttgcaca gcgtttgcag
    14401 tagcagcgtg gatttccccg cacctgggtt accgccaatc agaatggcgc ttcctggcac
    14461 cacgccgccg cctagtacgc ggtcgaactc tttaaatccg gtggaaaaac gcggcagctc
    14521 ttcaaggctg atatcggaga gtttctggac ttttgccacc ccggcgctac cggcatagcc
    14581 gctgagacgc tcgttacgcg ccaccgttgg cgacgcagca agacgcacct cggtgatggt
    14641 gttccaggca tgacaggcac tgcactgccc ctgccagcgc ggataatcgg ccccgcattc
    14701 attacaaaca aaggcgcgtt ttggagcttt tgccacggtg tacctcgtta atgctgtgcc
    14761 gcccgcagga tggcgggcga gcaattactt ctgattcagg ctgccagaga ggatgcagaa
    14821 tacccccatc aggtcagcgt gacggatggt gacttccgcc ttttcattca cttttggctt
    14881 ggcatggtag gcaatcccca gccctgctgc tttgatcatc ggcaggtcat tagctccatc
    14941 gccaatcgcc acggtctgcg ccagcgggat ttcatactcc tgcgcgaggc gagtcaaagt
    15001 tttcgctttg tactgcgcgt ctacgatgtc gccgatcaca ttgccggtaa atttaccgtc
    15061 catgatctcc agttcattgg ctaccacggc ggtcaggcgc agcttgtcgc gcagatattc
    15121 agcaaagaaa gtaaagccgc cggaggcaat cgccactttc cagcccagcg tttccagctt
    15181 gagcaccagt tgcgttaagc ctggcatcag cggcagattt tcacgcacct gttgcagaat
    15241 attggcgtca gcgcctttca gcgtcgccac acggctgcgc aggctggcgg taaaatcgag
    15301 ttcgccgcgc atcgcccgtt cggttacttc cgccaccatc tcgcccgttc cggccagttt
    15361 ggcaatttca tcaatacatt caatctggat ggcggtggaa tccatgtcca tcaccagcaa
    15421 acccggcgtg cgcaggtgcg ggattttccc cagcggggcg acatccagtt gcgcttcatg
    15481 ggccaggcgc gtagcccgtg cggtgagtga acctgccaga cgaatcacct gataatcttc
    15541 cacgcaccag gcggcaacaa tcaccatcgc cgcacccagt ttgctctggt attgggtcag
    15601 acgctgttta tccagcccac gaccatacag cagccagccg ctacgacctg cgtggtaatc
    15661 cagtggcatc acttcatcac cacttaatga aagaggcaga ccaggccata aagagacatc
    15721 ttcaggcagg tcgcaccagg taatgttagg cattaaggct cctgtaaaat cgttcgaagc
    15781 agggaaaata acgcatgagg ctaccttgta tccattgctt ctggcaacat taagtctcaa
    15841 attttcaaag ggtggaagat ggctcgcaca aaactgaaat tccggctgca tcgggcagtg
    15901 attgtcctgt tctgtcttgc cttgttagtg gcgctgatgc agggagcgtc atggtttagt
    15961 caaaaccacc agcgacaacg taatccacag ctggaagaac tggcccgcac cctggcgcgt
    16021 caggtaacgc tgaacgttgc accgctgatg cgtaccgact caccggatga aaaacgcatt
    16081 caggcgatcc tcgatcagtt aacggatgaa agccgtatcc tcgacgcggg tgtgtatgac
    16141 gaacaaggcg atcttatcgc acgttctggc gaaagcgtcg aagtgcgcga ccggctggcg
    16201 ctcgacggta aaaaagcagg cggctatttt aaccagcaga ttgtcgagcc aattgcgggt
    16261 aaaaacggac cgctcggcta tctgcgcctg acactcgaca cccatacgct cgccaccgaa
    16321 gcccaacagg tggataacac cactaacatt ttacgcctga tgttgctgct ctcactggca
    16381 atcggcgtag tgctgacccg cacgctgcta cagggtaaac gcacccgctg gcagcaatcg
    16441 cccttcctgt taaccgccag caaaccggtg ccggaagagg aagaaagcga gaaaaaagag
    16501 tgacccatta ctacaagaaa ggaaatcgtt atgtccacat tacgcctgct catttctgac
    16561 tcttacgatc cgtggtttaa cctggcggtg gaggaatgta tttttcgcca gatgcccgcc
    16621 acgcagcgcg ttctgtttct ctggcgcaat gccgacacgg tagtgattgg tcgcgcgcag
    16681 aacccgtgga aagagtgtaa tacccggcgg atggaagaag ataacgtccg cctggcgcgg
    16741 cgcagtagcg gtggcggcgc ggtgttccac gatctcggca atacctgttt tacctttatg
    16801 gctggcaagc cggagtacga taaaaccatc tctacgtcga ttgtgctcaa tgcgctgaac
    16861 gcgcttggcg tcagcgccga agcgtccgga cgtaacgatc tggtggtgaa aaccgccgaa
    16921 ggcgaccgca aagtctcagg ctcggcctat cgcgaaacca aagatcgtgg cttccaccac
    16981 ggcaccttgc tgctcaatgc cgaccttagc cgcctggcaa actatctcaa tccggataaa
    17041 aagaaactgg cggcgaaagg cattacctca gtgcgttccc gcgtgaccaa cctcaccgag
    17101 ctgctgccgg ggatcaccca tgagcaggtt tgcgaggcca taaccgaggc ctttttcgcc
    17161 cattatggcg agcgcgtgga agcggaaatc atctccccgg acaaaacgcc agacttgcca
    17221 aacttcgccg aaacctttgc ccgccagagt agctgggaat ggaacttcgg tcaggctccg
    17281 gcattctcgc atctgctgga tgaacgcttt acctggggcg gcgtggaact gcatttcgac
    17341 gttgaaaaag gccatatcac ccgcgcccag gtgtttaccg acagcctcaa ccccgcgccg
    17401 ctggaagccc tcgccggacg actgcaaggc tgcctgtacc gcgcagatat gctgcaacag
    17461 gagtgcgaag cgctgttggt tgacttcccg gaacaggaaa aagagctacg ggagttatcg
    17521 acgtggatag cgggggcggt gcggtaactc gctgattaaa atatccaagg aaggaaatat
    17581 gatcgatctg gaaaaccagg aacgggagat tatcaattta atgttcagcc agcgtatttc
    17641 ctggctagcc gccgtgcgta tcaggcataa attatcgctt gctgaagtaa gtaaaatgtt
    17701 aggtatctcg attaattctc taaaacagat cgaaaagacg gaacggctta gctcaaacat
    17761 caaaagcaaa atggcagaaa tttatggttg tccaccagag ttgctgatat gcccttcctg
    17821 gatgacggct gaacataaat gaatagattc gtgaatagat acttgaatgt attttatttt
    17881 ctatccatat gaaaaataat agtaaatcta taaatcagtt gctattgatg aatagatact
    17941 aaaaagccgg agcagtcccc cactccggct taacacgaca atcgcctttc agcgaaacac
    18001 aattactctt tatcgcccag cagaacggat tccagtgcga ttttgatcat gtcgttgaag
    18061 gtggtctgac gctcagcggc agtggtctgc tcgtgagtgc ggatgtggtc agacacggtg
    18121 cagatggtca gggctttcgc gccaaattct gcagcgacgc cgtagatacc agccgcttcc
    18181 atttccacgc cgaggatgcc gtatttttcc atcacgtcga acatttcgcc gtccggagag
    18241 tagaacaggt cagcggagaa caggttacca acgcgagcat caacgcccag tgctttagcc
    18301 gcgtctaccg cgttacgcac catgtcaaag tcagcgatag cggcaaagtc atgatcttta
    18361 aaacggatgc ggttaacttt ggaatcggtg caggcaccca taccgataac gacgtcgcgc
    18421 agttttacgt gcggcagaac tgcgccacag gaacccacgc ggataatttt cttcacgccg
    18481 aaatcggtga tcagttcttt ggtgtagatg gagcaggacg ggatacccat accgtgaccc
    18541 attacggaaa ttttgcggcc tttgtaagta ccggtgaagc ccagcatacc gcgaacgttg
    18601 ttcacttcac gggcatcttc aaggaaagtt tcagcaatat acttcgcacg cagcgggtcg
    18661 cctggcatca aaactacgtc agcgaaatcg cccatttctg cattaatgtg tggggtagcc
    18721 attgttttat ccttatcaac atgttttcac cctctccctg agggagaggg ccggggtgag
    18781 ggcatcaagc cgcacccatt atcagaacat ggctttgcca tattccatat cagaagtacc
    18841 aaaatatttt gccagagtct gaccaatatc cgcgaaggtt tcacggtgac ccagtgagcc
    18901 cggttttact ttcgggccgt aaaccagtac cggaatgtgt tcacgcgtgt ggtcagtacc
    18961 ggtccaggtc ggatcacaac cgtggtcagc agtgaggatc aggatgtcgt catcgcgcag
    19021 cagagacatc agctccggca gacggcggtc gaacagctcc agacccgcag cataaccggc
    19081 gacgtcgcga cggtggcccc aggaagagtc gaagtcaacg aagttggtga agacgatggt
    19141 gttatcaccc gcttctttca tctctttgat ggtggcgtca aacagcgcgt ccaggccagt
    19201 tgctttcact ttcttggtga taccgcagtt ggcgtagatg tctgcaattt taccgacaga
    19261 aaccacctga ccgtgttttt catcaaccag tttctgcagc acggtcggtg ctggcggctc
    19321 aacagccagg tcgtgacggt taccggtacg ctggaagtta ccggctttgt cgccaataaa
    19381 cggacgagcg ataacacgac cgatgttgta gccgccgttg gtcagctctt cacgggcgat
    19441 ttcgcacagt tcgtagagtt tatccaagcc aaaggtttct tcgtggcagg caatctggaa
    19501 cacggagtca gcggaggtat agaaaatcgg cttgccggtt ttcatgtgct cttcgcccag
    19561 ctgatccaga atgaccgtac cggaagagtg gcagttaccg agataaccag gcaggttagc
    19621 gcgttcaacc agtttatcca gcagctcttg cgggaagctg ttttcgtgat cggagaagta
    19681 tccccactca aacagaaccg ggacaccggc gatttcccag tgaccagacg gggtatcttt
    19741 accggatgac atttcgtgcg cccatgcgta cgcgccgata acttcagcgt tgccgtccat
    19801 tcctgccgga atgaaaccgg tagaaccttc atgtgctttc gccagcccca gacgggtcag
    19861 atttggcaga ttgagcgggc ctttacgacc gttatcagct tcgcctttgg cacaggcttc
    19921 tgcgatatga cccagggtgt cagccccgac gtcaccaaag cgttctgcat cttctgtagc
    19981 gccgatgccg aatgagtcca gcaccataat aaatgcacgt ttcatatgtt ctccgtactc
    20041 agtgcttcaa atttaaatga tcagatcagt ataccgttat tcgctgatac ggcgatagac
    20101 agttggtgtg ctttccggtg ctttatcggc aagtttaatt gccgctttca ccgctttcgc
    20161 cgcgtcctgc cagctgtttt cgtctttcgc gtggataaca gccagcggac gctgtccgtc
    20221 tacctggtcg cccagacgcg ccatatcagt aaagccgacg ctgtaatcga tggtgtcaga
    20281 tgcctgacgg cgtccgccgc ccattgcaac cactgccatc cccagcgcgc gggtatccat
    20341 ttcactgaca aaaccttcgg tatcagcata gactgctttc gtcagcatcg ctgtcggcag
    20401 atacttcgcg tagttctcaa cgaagtcggt cgggcctttt tgtgccgcta ccatacgacc
    20461 aaagacttct gccgctttac cgttgtccag caccgcctgc aatttcgcgc gcgcttcggc
    20521 gtcatctttc gccagtttgc cggagatcag catctccacg cacagcgcca tcgtgacatc
    20581 aaacagacgc gggttacgat attcacccgt caggaactgc accgcttcac gaacttcaac
    20641 cgcgttacct gcactggagg ccagtacctg attcatgtcg gtgagcagcg cggtggtgcg
    20701 cacgccagcg ccgttagcca cgccaacaat cgcttcggca agggcttcag agagttcgta
    20761 ggtcggcata aacgcgccgc tacccacttt cacgtccatc accagcgcat ccagaccttc
    20821 cgccagtttc ttcgccagga tcgaggcagt gatcagcggg atggagtcca cggttgcggt
    20881 aatatcacgg gtcgcgtaga aacgtttatc cgccggagcc agtgagctgg tctggccgat
    20941 aatcgctacg ccgacgtctt taataatttc gcggaaacgg ttgtcatccg ggaaaatgtc
    21001 gaagccaggg atggattcca gtttgtcgag cgtaccgcca gtatgaccga ggccgcgacc
    21061 ggagatcatc ggaatatagc cgccgcaggc tgcgaccatc ggccccaaca tcagcgaagt
    21121 cacatcgccg acgccgccgg tggagtgttt atcaacaatc gggccattca gatgcaggct
    21181 tttccagtcg agaacggttc ctgaatctcg catcgccatg gtcagcgaga cacgctcagg
    21241 cattgtcata tcgtggaaga aaatggtcat cgcgagggcg gcaatctgcc cttcggagat
    21301 agtgttgtcg cgaataccgt tgataaagaa acgaatttct tcatcgctta gcgcatgacc
    21361 atcacgtttt ttacgaataa tttcttgtgc gagaaacaag gtaacctccc gaggaaaaga
    21421 gtatgaggcg gcttttgccg gatgcgtcta cgccttatcc ggcctacggg tagatacgtc
    21481 tcgtaggcct gataagcgca gcgcattagg cgtaaagcat cttacttagt agctgctggc
    21541 gctcttaccg tcaccgtggc ccagcgcttt caacaggctt gccagcaggc tggaagcacc
    21601 aaagcggtag tgacgcgcat ctgcccagtc agcaccgaac agttcatctg cgatggcgag
    21661 atatttctgc gcatcttccg cagtacgcac gccgcccgct ggtttgaaac caacggtttt
    21721 ttctacgccc atatcacgga tcacttccat catgatgcgc gcgctttccg gcgtcgcgtt
    21781 cacagccact ttaccggtag aggttttgat gaagtccgca cccgctttga tggagatttc
    21841 agacgcttta cggatcagcg cttcgtcttt cagttcgccg gtttcgatga tcactttcag
    21901 cagtacattc gctgccgcgc aagcctcttt acaggctttc accaggtcaa aaccaacctg
    21961 ctcgttaccc gccatcagcg cgcggtacgg gaacacaacg tcaacttcat cagcaccgta
    22021 ggcgattgcc gcacgggttt ctgccagcgc gatttcgatg tcatcgttac cgtgtgggaa
    22081 gttggttacc gtagcgattc ggatttccgg ggtgccctgc tctttcagcg ttttgcgagc
    22141 aatcgggata aagcgaggat agatacagat agcggcggta ttgccgaccg gagttttggc
    22201 ctgatgacac agagcaatta ctttctcgtc ggtgtcgtcg tcattcaggg tggtcaggtc
    22261 catcaatttc agtgcacgca ggctgcttgc tttcagatca gtcatttcat tctcctggct
    22321 tgtcgccaat aaaattcacc ttgcgagttt gttagtattc taacatctac tccgcaacac
    22381 acttcgatat acatcacaat taaggaaatc tacttacaag tttgcatcac tgtaatgcga
    22441 tctggttcaa ataattcact ttcaaatgaa tgcgtcagtg gtggcaaacg catcaggatc
    22501 aaagtgaaca tcacgaaact tcttacaatg gcgcatcacc ttcggcataa aaggaaaaga
    22561 agatgcccac ttctcatgaa aatgcactgc aacaacgttg ccagcaaatt gtcaccagcc
    22621 cagtgcttag cccggagcag aagcgccatt ttctggctct ggaagcagaa aacaatctgc
    22681 cttatccgca gcttcctgcc gaagcccgcc gcgcgctgga tgagggtgta atctgcgata
    22741 tgtttgaagg tcatgcgccg tacaaaccgc gctatgtctt acccgattac gcccgttttc
    22801 tggcgaacgg ttccgaatgg ctggagctgg aaggcgcgaa agatcttgat gacgcactct
    22861 ctctgctgac cattctttac caccacgtac cgtcggtcac atcgatgccg gtctacctgg
    22921 ggcaactgga tgcgttgttg caaccgtatg ttagaattct aacacaagac gagatcgatg
    22981 ttcgaataaa acgtttctgg cgttacctcg acagaaccct gccagacgcc tttatgcacg
    23041 ccaatatcgg cccgtctgat tcgcccatta cccgtgcaat cttacgtgca gacgcagagt
    23101 tgaagcaggt ttcaccgaac ctgaccttta tctacgatcc tgaaatcacc cctgatgacc
    23161 tgctgctgga agtggcgaag aacatctgtg aatgtagcaa accgcacatc gccaacggtc
    23221 cggtgcatga taaaattttc acaaaagggg gctacgggat tgtgagctgt tacaactcac
    23281 tgccgctggc gggtggtggc agcacgctgg tacgccttaa cctgaaagcc attgccgagc
    23341 gcagcgaatc gctggatgac ttctttacgc gcactctacc gcactactgc cagcagcaga
    23401 tcgccatcat cgatgcgcgg tgtgaattcc tctatcaaca atcacacttc tttgagaata
    23461 gcttcctggt gaaagaaggg ctgattaacc ctgaacgttt tgtgccaatg tttggcatgt
    23521 atgggctggc ggaagcggtt aacttgctgt gtgagaaaga agggattgcc gcacgttacg
    23581 gtaaagaagc cgccgcaaat gaagtgggtt atcgcatcag cgcgcaactg gcggagtttg
    23641 tcgccaatac ccccgtgaaa tatggatggc aaaaacgcgc catgttacac gcacagtcgg
    23701 ggatcagttc cgatattggc accacgccgg gcgcgcgttt gccgtatggc gatgagccag
    23761 atccgatcac ccatctgcaa actgtcgccc cgcatcatgc ttattattat tccggcatca
    23821 gcgacattct gacgctcgac gaaaccatca aacgtaaccc gcaggcactg gtacagcttt
    23881 gcctcggtgc ctttaaagcc ggaatgcgtg aatttaccgc caatgtcagc ggtaacgatc
    23941 tggttcgcgt taccggttat atggtacgtt tgtcggactt agaaaaatat cgcgccgaag
    24001 gttcacgcac caacaccacc tggctgggcg aagaagccgc acgcaacact cgtattctgg
    24061 aacgccagcc gcgcgtgata agccatgaac agcagatgcg ctttagtcag taagattatc
    24121 cccttctcct gcgttgacgg gccaggcagt cgtctggctc tgtttttgca gggctgcaat
    24181 ctgcgctgca aaaactgtca caatccgtgg acgatgggac gttgcaatga ctgtggagag
    24241 tgcgtgccac agtgtccgca tcaggcgttg cagattgttg acggcaaagt gctgtggaac
    24301 gctgtggtct gcgagcagtg tgatacctgc ctgaagatgt gtccgcaaca tgccacgcca
    24361 atggcgcaat ccatgagcgt ggacgaagtg cttagccatg tccgcaaagc ggtgctgttt
    24421 attgaaggga taacggtaag cggcggcgaa gccacgaccc aactgccgtt tgtagtggcg
    24481 ctgtttactg ctatcaaaaa cgatccgcaa ctgcgccacc tcacctgcct ggtggacagt
    24541 aacggcatgt tgagcgaaac cggctgggaa aaattactcc cggtgtgtga cggcgcaatg
    24601 ctcgatctca aagcgtgggg gagcgaatgt catcaacact taaccggacg cgataatcag
    24661 cagattaagc gcagcatctg tttgcttgca gagcgcggca agctggcgga actgcgtttg
    24721 ctggtaattc cagaccaggt ggattatttg catcacatcg atgagctggc gacgtttatc
    24781 aagagacttg gcgatgttcc ggttcgcctg aatgcgtttc atgcccacgg cgtgtatggc
    24841 gaggcgcaaa gctgggcgag cgccacgccg gaagacgttg agccgttggc tgatgcgtta
    24901 aaggtgcgcg gggtgagccg gttgatattt ccggcgctct atttgtgaca tgccggatac
    24961 ggcctacggt tcggcacaga cttgtaggca tgataagacg cgacaagcgt cgcatcaggc
    25021 attatgcgcc gactgccgga tgcggcgtga acgccttatc cggcctacgg ttcggcacaa
    25081 acctgtaggc atgataagac gcgtcaagcg tcgcatcagg cattgtgcgc caactgccgg
    25141 atgcggcgtg aacgccttat ccggcctacg gttcggcaca aacctgtagg catgataaga
    25201 cgcgttaagc gtcgcatcag gcattatgcg ccgactgccg gatgctgcct acgaagggta
    25261 aactacccac taacgctaaa caacgcatac gtattattaa gcaacacttc ggcaatctca
    25321 tcttctggtt ccgggcgcaa ctcgcaaagc acgtcaaaca cccgcacagc ctgttccggt
    25381 cggttaggct gcccctgaaa gccgttgagc ggcatatccg gcgcgtcggt ttccagcaac
    25441 aacgatgcca gcggtaattt tgcgatgaca tcgcgggttt tactggcgcg tggataggtg
    25501 atagtaccgc ctacgccgat tttgtagccc agctgtacaa accgctcggc ctgttgcagg
    25561 ctgccggaaa aaccgtgaac cacgccagtg cacggtaaat cgtgacgttt aagatgcatc
    25621 gccagtttgt cgtgtgtacg ccgggaatga aggatcaccg gcagatcgta gcgtttcgcc
    25681 agtttcagct gttcgtcgag taaccactgc tgcctctcaa attgcggatc gtcgccgaag
    25741 agatccagac cgatctcccc caccgccacc accttcgccg gacgcctttc cagcgcctgc
    25801 tgtagctgat caagagacac atcgctatgt ttttccaaca tccccggatg caaccccaat
    25861 gcggcataca gcggttgata tttttccgct aacgcctgca cgcgggcaaa attttccgcc
    25921 tctgtcgccg gaacaatgat cttgcctacg cccgcttgtg ccgcgcgttg caggctggcc
    25981 tcttcatcgc cactaaacgg cgggaaatca aaatggcagt gggtatcgat aaaacggcaa
    26041 atcaagccag atcctcattg ttaaatgtcg ggtcgttcgc ctgcggagca tcgctcactt
    26101 cggcaaccag cgtatcgttg gcgactggcg caggcggaat aacgatcgat tccggcgtca
    26161 ccactggcac cagatggcgg gtaagcggcg ctttttcagt taatagcttg ccaaccgtgg
    26221 cgaggaaata acgaccgcat aaacgcccaa gtttatagtc ttcacgcagc gccggaatcc
    26281 gactgccaag cgcgatacta tgtaatggtt tcggtggata aatttcgaat atccgcagct
    26341 tgcccggtgg tttctcaata aattgctgaa tgtcacgata gctggtttca tgatgctgca
    26401 ccagattgac cagcggctgc aggctactgt cacccagcca gcgttccatg cgtttgaacc
    26461 actgcggcgt gtagtacatt tgtgacggca cagtgcggat gacgaccaac gttttagcgc
    26521 cctgccttgc cgcctcttta accggaatcg catcactgat cccgccatcc aagtagttaa
    26581 tgccttccag cgacactccg ctacgataaa agccaggtat cgcactggag gcgcgaatca
    26641 catccagcca gttttgtttg gttggtaaaa agtaattagg cgcgtagtca tcctgacgac
    26701 aggcgcacat ataaaacgat ttgccgctgt caaacaaccg cgcggcggta tccatttgca
    26761 acggcatctg gcttgcggtg gcctccacca gccagtcgag atcgataaga tttcctccac
    26821 ggacaaagcg caatggatcg aaaaattcgc gttttgtggt atagcgcatg atgactttgc
    26881 gcgcgtaacc gggctgattg cagataaacg ccgagaggtt ctgcgccccg gcagatgtgc
    26941 cgagataaag atcgaaagga ttaaactgcg cgcgcataaa ctcatccagc acgccagccg
    27001 tgaaaattcc acgctgtccg ccgccttcgc acaccagagc tattcgtcca ggctggaacg
    27061 gccttagcga caacggcgca atattaccaa gcgttacagg tattcgctgc cccacctctg
    27121 ccttcctgtt tttgttgtga aagaatgaca aagtaacgca atttattatt cgctaaaacc
    27181 gcaaaagcca gtccgctgga ctggctttgg ataaaatttt cttaatggtc tgaaatctag
    27241 gggcgttttc ggcccatgaa caaactcacc aggaacagaa taatcccgac gacaaagaca
    27301 attttagctg cgcctgcagc ggtaccggcc agaccaccaa acccaagtgc ggcggcgatt
    27361 aacgcgataa ccagaaatat gatgccccaa cgaaacataa gcgtctcctt taccatagtt
    27421 aatgtcaccg cttgatatga gcgtgaaaat cactcaatgc gatattttta gtgtggtgca
    27481 catcgcgcct cccgacaaag ttcgggagga cgaattacga cgaattactt agttttcaga
    27541 tcatttttaa cgcttttcac accatctacc gctttggcga tactttcagc acggtcgctc
    27601 tgtgcctggg aatcgacggt accggagagc tgaaccacgc cgtcggtggt ttcaactttc
    27661 acatgacggg aagggacgat atcgtccgcc agcagtttgg ctttgatttc actggtggtg
    27721 gcggtgtcac ccgcgtagcc cttcacagag ccttctttag cgtcgcgaac gtgcagtttg
    27781 tcgctgacag aggtcacccc ttcaacgcct ttcgccactt tcactgcctc ttcggcctgg
    27841 gcctggcttt caacgaaacc gctcagggtc acgacttttt gatcggtttt tacagagata
    27901 tcggtgctct tgatgttgtc atgatccacc agggccgcct tcactttcgc ggtgatggcg
    27961 ctgtcatcca tgaaattacc gactttattc atagagctat cgactttttg ccctgcgctt
    28021 tcattggtag tctgtgcgtt gttttccgcg taggcagagc cggtcgcgac ggcagaggtc
    28081 aacattacag ccagcagagt tttcgaaatc ttcagtcttg tcatagtcat cgatttattc
    28141 ctgtatgttt gctcgtaatt tgagcctggc aacacgaggt tgcattgctg aatagggaga
    28201 gacttcaccc tctacagaag tcaatggtcg ccatcacaaa agcgatgggt gatgaataac
    28261 gaccattaca gcctctgaat cagttattaa tatcggtaga atgacaatcg acggctttag
    28321 atactgatat ctacgcattg aacggtattt aacgccgtca gaaatgtcat tactttgtta
    28381 aatatagatc acaattttga aaccgctcgg gatatcagcg aaaacataag caaaagtgaa
    28441 tgttttaaga acattccgta agcggctaat aaggaaggga aattgacagg gcgaggcggt
    28501 tgccgcgccc tggaggcaag aattaatgct cgcgggtctg gtggaactga acgtccggat
    28561 aacgttcctg tgccaggcgc aggttaacca tgctggtagc gatgtaagcg agattatcgc
    28621 cgccatcaag cgccagttgg ctttcgttct tacgcttgaa ctcttcaaat ttcttcgcgt
    28681 ccgcacattc tacccaccgg gcggtggcaa cgttgactga ttcatatact gcttcaacgt
    28741 tgtattcgct cttcaggcgc gataccacta catcaaactg cagcacacca accgcaccaa
    28801 cgatcaggtc gttgttggag atcggacgga acacctgcac cgcgccctct tcggaaagct
    28861 gtaccagccc tttgagcagc tgtttttgct tcagcggatc tttcaggcgg atacgacgga
    28921 acagttctgg tgcgaagttc ggaataccgg tgaacttcat catctcaccc agggtaaagg
    28981 tgtcgccgat ctgaatggtg ccgtggttgt gcaggccgag gatatcgcca ggatacgctt
    29041 cttcaacgtg cgaacggtca cccgccataa aggtcagcgc gtcagagatc accacgtctt
    29101 tcgcggtgcg cacctggcgc agcttcatgc ctttttcata tttaccggat accacgcgca
    29161 taaacgccac gcggtcgcgg tgtttcgggt ccatgttggc ctgaatttta aatacgaagc
    29221 cggtaaattt gtcttcgctc gcttgtacgg tacgggtatc agtctgacgc ggcatcggcg
    29281 caggtgccca ctccaccagg ccatccaaca tatgatcaac gccgaagtta cccagcgcag
    29341 taccgaagaa taccggagtg atttcgcccg caaggaacag ctctttgtcg aactcgttag
    29401 acgcgccttt aaccagttcc agttcgtcac gcagctgctg tgccagatct tcaccaaccg
    29461 cagcatcgag atccgggtta ttcagccctt taacaatgcg gacttcctga atggtgtgcc
    29521 ctttaccgct ctgatagaga taggtttcat ctttataaag gtggtaaacg cctttaaaca
    29581 gcttgccgca gccaattggc caggtgatcg gtgcgcagcc aattttcagc tcgttctcaa
    29641 cttcatcgag caattccatc gggtcgcgga tatcacggtc aagtttgttc ataaaggtga
    29701 ggatcggcgt gtcgcgcaga cgggtaactt ccatcagctt acgggtacga tcttcaacac
    29761 cttttgcggc gtcgataacc atcaggcagc agtccaccgc cgtcagggta cgataggtat
    29821 cttccgagaa gtcttcatgc cccggggtgt cgagcaggtt aaccaggcaa tcgtgatacg
    29881 gaaactgcat cacagacgta gtaatggaga tcccacgctg cttttccatc tccatccagt
    29941 ccgacttagc gtgctggttg gaaccacggc cttttactgt accggcggtc tgaatggcct
    30001 gtccgaacag cagcaccttc tcggtgatgg tagtcttacc ggcgtccggg tgagaaataa
    30061 tggcaaaagt gcggcgcttc gccacctctt gcaaataagg agacaacgtc ataattcttt
    30121 cttcttgagt aagcggcatc gcgccgcgct tgttgaaaac gaaaaattgc ggctatttta
    30181 cccatcaacg ggggggaggc aatcagtgtt tacacaggag ctgctccagt tcatgcaacg
    30241 aagaaacggt ccaggtgggc gcgatgcctt ctggttgctc gcgatggtgt gcattcagcc
    30301 agcaggtcgc aagcccggcg ttgatgccac cgagaatatc ggactcggca gtgtcgccaa
    30361 ccatcagcac gcgtgaacgg tcaggattgc ccgcctgttc cagcgcataa tcgaaaattt
    30421 tcttattcgg tttagcaacg ccaacttctt cggaaatcac cagcaaatcg aagtaatcac
    30481 gcaggcccgt ccgttccaga cgcacttgct gcaaggcgct aaaaccgttg gtgatgatgc
    30541 cgattttagc gttgccacga atggcgttaa gcagagaaac cgcgcccggc aacggcgtac
    30601 agatttccgc catcgcatta ataaaggcat cgttaagcaa gcctgcttca actttcagcc
    30661 gttcggccca gctctcaaac cgcccgtgct gaagctgtaa tgaagtgatc gcgccgtttt
    30721 gataatccac ccacagtggc ttgttaacgg cctgatagtc ctgaaaatct tcagcggtaa
    30781 aggtgacgct gtaatcaaga aacatccgct gcaggccggt gaatgagtca aaggtaaaca
    30841 gcgtttcatc ggcatcaaag aaaatccagt cccacttcat tattccacct tgtcttacat
    30901 actgattggc aacgccatga tgatggcgtc ttcgcgaccg tccgtggtgg ggtagtaatt
    30961 gcggcgaatc gtcgcctcgt taaagcctaa actttcgtac agggcaatgg cggcagcgtt
    31021 tgaagcacgg acttccagcc atagtgtcgc cacgccgcgt ttttccagtt cgtcgatcag
    31081 atgttccagc agcacccttc ccaatccctg acgctgatag tcaggatcga ccgcaatatt
    31141 gaacaatgta gcttcatcca gcaccacttg cgtaatcgca aacgccgcca ttttgccgtt
    31201 ttgcgttaac tgaaagttga gataacgctc gccctggttg ctggcaaagg ttttttcact
    31261 ccacggaaag gcgtgggcgc gttgttcaat gtggtaagcc gctggtaaat cagtcgtttc
    31321 gagggaagaa atcgtgttca tatgtgcaaa tctgttgcca taacgcggcg cgtgccgttg
    31381 ggtttgcccg taattcggtg agcgccggtg atgccacctg agcgccttcc agtgatagcg
    31441 gttcgtcagt acccaaccgc cagctgttgc agcgactgcc ttgcggcagc atcgcgattt
    31501 tttctggcgt cagttgcagc acttgatcgg ggctgacggt taatgcgcgc agaacatcgc
    31561 tcactaaagg atcggtcagg gcgggaagat cgtttgccac catcaccaga cggacgtgtg
    31621 ccgggatcgc aatggcaatc tccccctgca acgcgccagg gcgacgcagc gaccactggg
    31681 tgatgcccag ttgctgtaac tgccagtctc gtcgggatgt catagcgaat cgctcctgtt
    31741 gtcaggggcg caaatatagc aaattcgtcg ataccgcgcc aacatatggc tataatcgcc
    31801 gccagtatca attgaggagc attccatgtc tgcatttacc ccggcaagtg aagtcttgct
    31861 gcgtcacagt gatgatttcg aacaaagccg tattctgttt gccggagact tacaggatga
    31921 cctgcccgcg cgtttagata ccgcggccag ccgtgctcat acccagcaat tccaccactg
    31981 gcaggtatta agccgccaga tgggggataa cgcccgtttc agtctggtcg ccacggtgga
    32041 tgacgtcgca gattgcgata cgctgattta ctactggccg aagaacaaac cggaagccca
    32101 gttccagttg atgaatttac tttctctgct gccagtgggg acagatattt ttgtcgttgg
    32161 cgagaaccgc agcggcgtgc gcagcgccga gcagatgctg gcagattatg cgccgttgaa
    32221 taaagtcgac agcgctcgtc gctgtggcct ctattttggt cgtctggaaa aacagccggt
    32281 atttgatgcc gataaattct ggggcgaata cagcgtcgat ggcctgacgg tcaaaacgct
    32341 gcctggcgtg tttagccgcg acggtctgga tgtcggtagc cagttgctgc tctcgacgtt
    32401 aaccccgcac acgaaaggta aagtgctgga tgtcggctgt ggcgcgggtg tgctttcagt
    32461 tgcctttgcg cgccattcgc cgaaaattcg tctcaccttg tgcgatgtct ctgcgccagc
    32521 ggtagaagcc agccgcgcaa cacttgcggc caacggtgtt gaaggtgaag tctttgccag
    32581 caacgtcttt tccgaggtga aaggtcgttt tgatatgatc atctccaacc cgccgttcca
    32641 cgatgggatg caaaccagcc tggatgcggc gcaaacgctg attcgcggcg cggtgcgtca
    32701 tcttaatagc ggcggcgagc tgcgaattgt agcgaacgcc ttcctgcctt acccggacgt
    32761 gctggatgag acatttggct tccatgaagt gattgcgcaa accgggcgtt tcaaggtgta
    32821 tcgcgccatt atgacccgcc aggcgaagaa aggttaatta tctcgccgaa taccgtgtcg
    32881 gatgcggcgt gaacgcctta tccgacctac aaaacctgca cgttagccct tcgtaggcca
    32941 gataagacgc gccagcgtcg catctggcat ctgcccccgc atgcctacca aacctgcacg
    33001 ctagtttcct gatggacatt tttccagcaa ttacacctcc ggcgataatt aactattgac
    33061 gaaaagctga aaaccactag aatgcgcctc cgtggtagca attcttttta agaattgatg
    33121 gtatgcgaag gtggcggaat tggtagacgc gctagcttca ggtgttagtg tccttacgga
    33181 cgtgggggtt caagtccccc ccctcgcacc aattatcttt acttcctttc ttgtttcttc
    33241 cttgattttt catccctgat tcaaattcat tatcatcagc atgatgccag caataagcgc
    33301 caggcacgat ggtagcaaaa cgtaatgccg taactgtttg tgatacagca tcaccggcgt
    33361 caatactaat cccatcagaa tgacaatccg ccactgatcc ggcgataaat ccgcatacat
    33421 cagccctgca atcagcaatc ctggcagcaa cactccccag ccactgccca gcatacgttg
    33481 caacatggtt tcatctcttt cattgataat gataaccaat atcatatgat aatttttatc
    33541 atttgcaagc cagagaaatc ccttgctatc gggtaaacct atcgctagga ttagcaatca
    33601 ttatcattta gattactatc ccgattatgg cctatcgttc cgcaccgctc tatgaagata
    33661 tcatctggcg aacgcatctc cagccgcagg atgctgggct tgcacaagcc gtacgtgcga
    33721 cgatcgccaa acatcgtgaa catttgctcg agtttatccg cctggatgaa cccgctccgc
    33781 ttaacgccat gacgctggcg caatggtcat caccgaatgc gctaagttct ctactcgcgg
    33841 tctattccga tcatatctat cgcaaccaac caacgatgat ccgcgagaac aaaccgctga
    33901 tctcactatg ggcacaatgg tatatcggcc tgatggtgcc accattaatg ctggcgctac
    33961 tgacgcagga aaaggcatta gatgtgtcgc cggaacattt ccatgctgag tttcacgaaa
    34021 ccggacgcgt cgcctgtttc tgggtcgatg tgtgtgaaga taaaaacgca acaccacatt
    34081 cgccgcagca gcgaatggaa acgttaatca gccaggcgct ggttccggtt gtgcaggcac
    34141 tagaagcgac cggagagatc aacggcaaac ttatctggag taataccggt tatttgatca
    34201 actggtatct cactgagatg aagcaactgc tcggcgaggc gacggttgaa tcgctgcgcc
    34261 atgccctctt ttttgagaaa acgcttacta ccggtgaaga taatccactc tggcgtaccg
    34321 tggtgctacg cgacggcctg ctggtgcgcc ggacctgctg ccagcgttat cgcttaccgg
    34381 atgtgcagca atgtggcgat tgtacgctga aatgaaagca ctgccgggga agtaaacccg
    34441 gcatcatgcg gattaatagg gatgcaacac attacttgct tcgcaatgcc gcatacataa
    34501 cagaatatct gcggcctcca acaaccctgc atcagaactg acgcccagtt tcgacatcac
    34561 attaaattta tgtgcacgga tcgttttgat attgcgttta agctgctcgg caatttgtgt
    34621 cattgagtag ccacgcgaca taaagcgcaa tatttctctc tccgtcgggc ttagcgtccg
    34681 gctttggtta atgtaccact gattgttcag tcggtcggtc gcctgacgta caccatttaa
    34741 tgacaaaaag agttcctgat gaaaaatctc cagcgttgac gctttactta atacaccgtc
    34801 cagcggtgat ggcgacaatg aaccaatcag ccgagcttca atatcatcat ccgcaataac
    34861 taaacgccgg gtacgcggaa acttaatcgc cagttcagtc aggcaagata atccctctct
    34921 gcgctcactt ctcatggcag aaaaagaaaa aatgaccgcc gcaaatgaga tccgcgacat
    34981 ggcatcctga aatgactcct gactggtata ggtatggagc ttataagggt tacccgcaaa
    35041 ctttctgaat aaactctcaa taccgatact gctcatgacg catttttcta tgagtgcgac
    35101 atttctcttc ttaattcggc tgtgttccat tctgcggcat ctccataaac agaaggcaag
    35161 ttaagttctc ttgcaccctg cgcgctattt atccatgcat acatttcagc gttgctgtga
    35221 attgccagtc gacgcatcgc actgtttttt tgggcgctga tagttttatt acttttctta
    35281 agcaacgaag ctatctggtt gatgccccat cctttaccta atagccgcag taccttgcgc
    35341 tctgaaagag tgagaatgac ggagcgatag ctaaaatcat gagtttcatt cattgccgga
    35401 gaggtcagca tggcgctgat acgctctgcg tgcgtattgc cggaacgtac ggtcttgacc
    35461 agattctcaa tgggttcaac atcagacaat aacgtcgccg tagggcacat gagcaattct
    35521 actgcctggg aataccagga gcgtgacacc atgaaaaccc agtgaatttc ccgatattgt
    35581 gagattaaag aataataatg ttcacaaaca gaacgaggat cttcactttc accggctaaa
    35641 tcagcaatga ctaatccgga acgacgtaat tgtaataagg taaggtcctc cgcagaggcg
    35701 ctggatatta tttcatattc agggaagtga gtcctcatga cctcttttaa ccctgcttgc
    35761 ataacaggta ttttactgat aacaattcca tttttgcagc atcctggcaa catatttcct
    35821 ccacatccac tgtctaaaaa tcatgatggt ttattcagga tattcattcc ttaaatgttt
    35881 ccagcaaatt aatagattaa gcgaataaat tatccttagc attcactttc atatcagcat
    35941 actcctgctg cacaacaact ccactgcatg tgtcagaagg ttagcatcaa atgataacct
    36001 ggtgaaatag cctggagaca tttaa
//
LOCUS       DAZGVQ010000034        34757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000034 DAZGVQ010000000
VERSION     DAZGVQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.034"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..685)
                     /locus_tag="ACHLAG_001643"
     CDS             complement(<1..685)
                     /locus_tag="ACHLAG_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFP0654893.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGK"
     gene            complement(696..1697)
                     /gene="ltaE"
                     /locus_tag="ACHLAG_001644"
     CDS             complement(696..1697)
                     /gene="ltaE"
                     /locus_tag="ACHLAG_001644"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFP0654894.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1734..3452)
                     /gene="poxB"
                     /locus_tag="ACHLAG_001645"
     CDS             complement(1734..3452)
                     /gene="poxB"
                     /locus_tag="ACHLAG_001645"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFP0654895.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDGLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3585..4553)
                     /gene="hcr"
                     /locus_tag="ACHLAG_001646"
     CDS             complement(3585..4553)
                     /gene="hcr"
                     /locus_tag="ACHLAG_001646"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFP0654896.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4565..6217)
                     /gene="hcp"
                     /locus_tag="ACHLAG_001647"
                     /gene_synonym="priS"
     CDS             complement(4565..6217)
                     /gene="hcp"
                     /locus_tag="ACHLAG_001647"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFP0654897.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKTREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6361..7260)
                     /gene="lysO"
                     /locus_tag="ACHLAG_001648"
     CDS             complement(6361..7260)
                     /gene="lysO"
                     /locus_tag="ACHLAG_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFP0654898.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHTTEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(7755..8450)
                     /gene="aqpZ"
                     /locus_tag="ACHLAG_001649"
     CDS             complement(7755..8450)
                     /gene="aqpZ"
                     /locus_tag="ACHLAG_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFP0654899.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFSELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            8876..10534
                     /gene="ybjD"
                     /locus_tag="ACHLAG_001650"
     CDS             8876..10534
                     /gene="ybjD"
                     /locus_tag="ACHLAG_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFP0654900.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(10531..11487)
                     /gene="ybjX"
                     /locus_tag="ACHLAG_001651"
     CDS             complement(10531..11487)
                     /gene="ybjX"
                     /locus_tag="ACHLAG_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFP0654901.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIIPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            11638..12753
                     /gene="macA"
                     /locus_tag="ACHLAG_001652"
     CDS             11638..12753
                     /gene="macA"
                     /locus_tag="ACHLAG_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFP0654902.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            12750..14696
                     /gene="macB"
                     /locus_tag="ACHLAG_001653"
     CDS             12750..14696
                     /gene="macB"
                     /locus_tag="ACHLAG_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFP0654903.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(14769..14993)
                     /gene="cspD"
                     /locus_tag="ACHLAG_001654"
     CDS             complement(14769..14993)
                     /gene="cspD"
                     /locus_tag="ACHLAG_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFP0654904.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            15143..15217
                     /gene="yljB"
                     /locus_tag="ACHLAG_001655"
     CDS             15143..15217
                     /gene="yljB"
                     /locus_tag="ACHLAG_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFP0654905.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            15316..15636
                     /gene="clpS"
                     /locus_tag="ACHLAG_001656"
     CDS             15316..15636
                     /gene="clpS"
                     /locus_tag="ACHLAG_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFP0654906.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            15667..17943
                     /gene="clpA"
                     /locus_tag="ACHLAG_001657"
     CDS             15667..17943
                     /gene="clpA"
                     /locus_tag="ACHLAG_001657"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFP0654907.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(18287..18374)
                     /locus_tag="ACHLAG_001658"
     tRNA            complement(18287..18374)
                     /locus_tag="ACHLAG_001658"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18338..18340),aa:Ser,seq:gga)
     gene            complement(18628..18846)
                     /gene="infA"
                     /locus_tag="ACHLAG_001659"
     CDS             complement(18628..18846)
                     /gene="infA"
                     /locus_tag="ACHLAG_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFP0654908.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(19131..19835)
                     /gene="aat"
                     /locus_tag="ACHLAG_001660"
     CDS             complement(19131..19835)
                     /gene="aat"
                     /locus_tag="ACHLAG_001660"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFP0654909.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(19877..21598)
                     /gene="cydC"
                     /locus_tag="ACHLAG_001661"
     CDS             complement(19877..21598)
                     /gene="cydC"
                     /locus_tag="ACHLAG_001661"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFP0654910.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDPQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMSEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(21599..23365)
                     /gene="cydD"
                     /locus_tag="ACHLAG_001662"
     CDS             complement(21599..23365)
                     /gene="cydD"
                     /locus_tag="ACHLAG_001662"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFP0654911.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(23488..24453)
                     /gene="trxB"
                     /locus_tag="ACHLAG_001663"
     CDS             complement(23488..24453)
                     /gene="trxB"
                     /locus_tag="ACHLAG_001663"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFP0654912.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            24998..25492
                     /gene="lrp"
                     /locus_tag="ACHLAG_001664"
     CDS             24998..25492
                     /gene="lrp"
                     /locus_tag="ACHLAG_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFP0654913.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            25627..29694
                     /gene="ftsK"
                     /locus_tag="ACHLAG_001665"
     CDS             25627..29694
                     /gene="ftsK"
                     /locus_tag="ACHLAG_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP0654914.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPV
                     APQPQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPVAPQQQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDTESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            29849..30460
                     /gene="lolA"
                     /locus_tag="ACHLAG_001666"
     CDS             29849..30460
                     /gene="lolA"
                     /locus_tag="ACHLAG_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFP0654915.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            30471..31814
                     /gene="rarA"
                     /locus_tag="ACHLAG_001667"
                     /gene_synonym="mgsA"
     CDS             30471..31814
                     /gene="rarA"
                     /locus_tag="ACHLAG_001667"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFP0654916.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            31905..33197
                     /gene="serS"
                     /locus_tag="ACHLAG_001668"
     CDS             31905..33197
                     /gene="serS"
                     /locus_tag="ACHLAG_001668"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFP0654917.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            complement(33437..33577)
                     /locus_tag="ACHLAG_001669"
     CDS             complement(33437..33577)
                     /locus_tag="ACHLAG_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0654918.1"
                     /translation="MPQKTIIVSILCLTMLLTVWMIHRSPCEFRLNIMWSEFAAFLQC
                     KP"
     gene            complement(33909..34049)
                     /locus_tag="ACHLAG_001670"
     CDS             complement(33909..34049)
                     /locus_tag="ACHLAG_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0654919.1"
                     /translation="MPQKTIIVSILCLTMLLAIWMIHRSPCEFRLNIMWSEFAAFLQC
                     KQ"
     gene            complement(34184..34486)
                     /locus_tag="ACHLAG_001671"
     CDS             complement(34184..34486)
                     /locus_tag="ACHLAG_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFP0654920.1"
                     /translation="MFHCPFCGASARIRTSKAANDTGTIRYKYYQCNNLECSATFSTM
                     ESVHRIIGQPQLIFHDKDIPRETMRKSHQGRSQLTLQLEDCGMALKPVHYARVPFG"
     gene            complement(34531..>34757)
                     /locus_tag="ACHLAG_001672"
     CDS             complement(34531..>34757)
                     /locus_tag="ACHLAG_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFP0654921.1"
                     /translation="LQRGAAEFSITLARGRAELYPEMHGTVTGFKTEIDNQDWLIAKV
                     EHNIDGSGFTTRLELEARIPEWIAEKESNG"
BASE COUNT         8607 a         8814 c         9267 g         8069 t
ORIGIN      
        1 gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc acctctggtc
       61 cggcggcttc gaagatgcgg tgttcgctgg ccggatgatc taacaacgcc accagatagt
      121 gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt ggcggcgtta
      181 acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa cctgcgccaa
      241 cgataattcc ggcccgaagt tcggtcacag gtacattcgc ttcacgaaga atgtccgccg
      301 tagcctgacg agcacgcaga tgatccgact gctcatgtgg cggggcctgc aacgaactga
      361 gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg agagccacct
      421 ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga aaatagacgg
      481 tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg actttatggc
      541 aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc gccgccagga
      601 tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag ccactggcac
      661 cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa tgcgcgccag
      721 tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac caggcggaca
      781 atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa cgcggcagca
      841 ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac atccgcgcct
      901 gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg caaccgtgcg
      961 acgttatttt tcagggcata catcccggcg gcagccagaa tcccggactg acgcatcccg
     1021 ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt accgacgagt
     1081 aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga atcacaatat
     1141 tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat gcgcgcaccg
     1201 tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt caggtattca
     1261 cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt gcgggcgaaa
     1321 tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt gccgtcggca
     1381 gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc ggcttcaaac
     1441 agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca gtgactgagc
     1501 agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc tttaccggaa
     1561 agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta aacgtcgtcc
     1621 ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt aacggtatca
     1681 ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc tttttacctt
     1741 agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat aattgcgcgc
     1801 aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg aatggctaat
     1861 tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa ggcgcgttgc
     1921 agggcttcat caacttcaga cgctttttct acacggatac ccgtaatgcc gcacgcttcg
     1981 gcaatgcggg caaagtttgt gtcgtgtagt tcggtgccgt cagtcaaata gccaccagct
     2041 ttcatctcca tcgccacaaa gcccagcacg ctgttgttaa agacgacaat tttcactggc
     2101 agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc gccatcgccg
     2161 cacatggcga ccacctgacg ttctggctct gtcgcctgcg cacccagcgc ctgcggcatg
     2221 gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc gttcattttt
     2281 agataacgtg ccgcccacac cgttggcgta ccaacgtcac aggtgaaaat agcgtcatcg
     2341 gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt ctcgctcggt
     2401 ttagctaaat cgtccagccc tttgcgggcg tcgcggtaat cttccagcgc tttatccaga
     2461 aacttgcgat cggctttttc ttccaccaat ggaagcaatg cacgcagagt cgacttgata
     2521 tcgccgacca gtgccatatc caccttgctg tgagcgccga tgctggctgg gttgatatca
     2581 atctgaatga ttttggcatc ggtcgggtag aaggcgcggt agggaaattg cgtgccgagt
     2641 agcactaacg tgtcggcgtt catcatggta tggaaacctg acgagaagcc gattaacccg
     2701 gtcattccaa catcatacgg attatcgtat tcgacatgtt ctttaccgcg cagggcatga
     2761 acaataggcg ctttaatttt cccggcaaac tcaactaact ctttatgcgc ccccgcgcag
     2821 ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc cagtttgcgt
     2881 aactcttctt cttccggcgt cacgactggt tgtggcgcat gataccagtg cgtggttgcc
     2941 ccttctggcg caggttttaa cgccacgtcg cctggtaaca cgacaaccga aacgccacgg
     3001 ttaagcaccg ctttgcgcat ggcaatcgcc agtacttgtg ggatctgctc cgggctggaa
     3061 accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt ttcctggaaa
     3121 tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac cggaacgtga
     3181 ttgcggtggc aatcgaacag gccgttgatt aagtgcaggt tgccggggcc gcacgatccg
     3241 gcgcagaccg ccagttctcc gctaagttgt gcttcagcgc cagcggcaaa ggccgccact
     3301 tcttcgtggc gggtggacat ccactcgatg gtgcccatgc gattaagacc gtcactaaga
     3361 ccgttcagag agtcgcctgt gactccccag atgcgtttca cccctgccga ttcgagtgtt
     3421 ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa tgtgataacg
     3481 gtaacaagtt tagttcatct gacggagggg gaagggatgg gagagaaagg aggcactaac
     3541 ggttaaatag cccgatgaaa ggaatatcat cgggcataag gcgattatgc gagaaccaaa
     3601 tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc ggcgtcggtc
     3661 agcgtcattg tactgctcac cgtatattca ccggaaacca cttttgtctt acagcagccg
     3721 caaacacccg cacggcaggc agcgacaacc ggaacgttat tgctttccag cgcctccagt
     3781 agcgtggtgc caaccggggc gtaaaattct cgtgccggtt gcagtttggt gaatttcaga
     3841 ccgctggtcg ccgcttctgc tactggggtg aagaatttct ctttaaagaa acgcgtcacg
     3901 ccgagcgctt tcacttcctg ctctacccaa tccatatacg gagccgggcc gcaggtcatc
     3961 acggtacgtg aagctaagtc aggtacacct gccagcagtt cgcgagtgag acgaccagcg
     4021 ataaagcctt cggtaacgtt attttctgcc accagcgtta ccggatagtt acgccactca
     4081 tcggcgaaaa taacatcctg cggcgtacgc acgttgtaga tcacccgcac atcggcctgt
     4141 ggacggttct tcgcaagcca gcgacgcatc gacataatcg gcgtgacgcc gcagcctgcc
     4201 gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc catcgcgtcc
     4261 gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccactggga gccgacaccg
     4321 tcatcaatcc gccgcacggt cagggtgata tattcactca cgcctggcgt ggaggaaatg
     4381 gtgtaagcac gcagcgtttc cgctgagttt cgcacgctga ccagtgcata ttgcccggcg
     4441 cgatatgggt agtaatcgtg gcaaatcagg gaaatcgtcc acacatccgg cgtttcttgc
     4501 gtaatgtgat gaacctgcat ccgccacggg cattgattcg ttggcatcgt catcgacaaa
     4561 ctccttacgc gctcaacagt tgcttcatgt cttcttcaac agtggtgata gaacgcaggc
     4621 cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt gcagtcgggc
     4681 cggtgacgat atttttcacg cccagagaaa gcagcgtcag cagaatgacg atcgctttct
     4741 gttcaaacca ggagagcacc agcgacagcg gcagatcgtt cacaccacag cccagtttct
     4801 ctgccagagt gacagccaga ataattgctg agtaagcatc gttacattga cctgcatcta
     4861 ccagacgcgg cagaccttcg atatcgccaa actcaagttt gttaaagcga tatttaccac
     4921 aggcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg gtgaagtagt
     4981 ggcgctcgcc gcgtgcgccg tcacagccac caagcaggaa gatatgacgc agtttttcac
     5041 ggctaaccag atcaatcagc gtatcggctg caccaagcag cgtctggcga ccaaagccca
     5101 cggtgatcag gtgaggaatt tcgctgtacg ggaagcccgc catttgttgc gcctgggcga
     5161 tgaccgcaga gaaatcttcg ccatccagat gacgcacgcc aggccagcca acgatgctgc
     5221 gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag ttcgaggtca
     5281 tcacgatggg gccagggaaa cgagcgaact ctacttgctg attctgccag ccgctgccgt
     5341 agttaccgac cagatgcttg aatttacgca gctccgggta gccatgcgca ggcagcattt
     5401 cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg ttgtagagat
     5461 ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg acgttgacct
     5521 gggttggcgt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg ctcatcactt
     5581 tgaagttcat ctggccgatt tccattgaac actcaagaag cgcgttcata tcggcaggcc
     5641 aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg tcgtattgac
     5701 cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac aggcacagca
     5761 gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg gtaaattctg
     5821 ctgcctgacg ttgcagctcg ccgagatcgt cgctcaccag ttgcaggtca gccatcgggt
     5881 tatcgacgcg cgcgttggca tctacagcca ggcattgcgc tttcagcgcc tcgcgcaggg
     5941 caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg ttggtcaggg
     6001 ttgagaaaaa ggcacgtggc gcgaagctgt caacatcgtg gttgatgatg ccgtattcac
     6061 gcgtttttac cgcccaggca gaaagccctt gcagcgccgc gatgagtaaa tcctgaaggt
     6121 cagaagtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg tttcctgccg
     6181 gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt aaagttatat
     6241 ttaatataca tgtttaaggt taagacgctt aacgcgggga taaaagggat ttttcatgca
     6301 actttaaggg agattgattt agcgcaattt tggcggcagg gatctaccgc cagagaggta
     6361 ttacgcagag aaaaaggcga tgaggatcgg cactaacagg ctaagaataa aaccgtgaac
     6421 aattgccgcc gggaccatat ccagcccgcc agtacgttga agaacgggca gggtgaaatc
     6481 cattgatgtg gcaccgcata agcccagtgc agtagagcgg ctgcggcgaa tcagcccagg
     6541 gatcaacata atagcaatca gttcacgggc cagatcatta aaaaacgccg cgctcccgat
     6601 taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc cgaaaccgga
     6661 ggccattgcc agcgcggtat tgatggggag atcaagaata aaggcgttaa ttaaaccacc
     6721 aattaatgaa ctgacaacca ccaccacggc gacaatcatt ccccggcgat taaggacaat
     6781 ctgctttaag gtcatgccat tattgcgcaa ctgaatacca acgaggaaaa gtagcaaaat
     6841 taacgtgtat tcactggctt cggtcgtgtg ttgtaagaaa gccagtccac tcagaccaat
     6901 ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca tcgcaatacg
     6961 cgacgggagt ttttcttgct gatggtggtt gcgccacggc aggcctcgct ccagccacat
     7021 cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat aatgcagaat
     7081 cgccaacagg ttactggcga ggttatcgag aaacgccaga ctgataccca taaaaaagag
     7141 aataaggtaa accatccagc ttaatagctg attaataact tttaacgcag cttgttggcg
     7201 aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc cagaaaacat
     7261 gaaaacccag tccttgcaaa gatgaagtcg aaatgcgcta tgacacacta ctgaaagcgg
     7321 aaggacgagt aaagttgcaa ttaaaaggaa atgttatgca taaggagcag tagagtattc
     7381 gttttcattt aaagatattc ttgcgcttta attacaaact gcaccgatgt tggtggcgtc
     7441 aaaatcgccg aggcgttccc tgaaggccgg ggcagcccac atggatgtgg gctgagggcg
     7501 cgttttacag ggatgttacc tcgcgcccga cccggtagcc gtaagggata agtcgagggc
     7561 accgcgcagc ggcgattttg ttcgccagag cccgggggtg catggggcgg cggcgattgg
     7621 ccgccccctg cgcgctcctt gcgccagtgg caatatgtgg cttagctcat gaaaggagcg
     7681 caacaagatg atgaatcaac atataacaac atcttaaaaa aaggcctgac attacgccag
     7741 gccttctgcg ttaattaatc acgcttttcc agcagggtcc ggtaaatcag accaccgata
     7801 atgccgccga caattggcac cacccagaag aaccacagtt gttctaatgc ccagccgccc
     7861 tggaagatag caaccgcggt gctgcgcgcc gggttaacag aagtgttagt caccggaata
     7921 ctaattaagt gaatcagggt taaggccaga ccaatagcga tcggcgcaaa acctgccggc
     7981 gcgaatttgt cggttgcgcc gtggatcacc aacaggaaac ctgcactcaa taccagttca
     8041 actaccagcg cggaaagcat ggaataaccg cctggtgaat gctcgccata accgttagaa
     8101 gcaaaaccgc tggctgccgc gtcaaaaccc gttttaccac tggcaattaa atacagcagc
     8161 gccgctgcaa caataccgcc gacaacctgg gcaattacgt agccaacgac ttcttttgcc
     8221 ggaaaacgtc cgccagccca taaaccaata gtgaccgccg ggttaaaatg accaccagaa
     8281 atatgaccaa cagcaaaggc catcgtcaga acggtcagac cgaacgccaa cgccacgccg
     8341 gcaaaaccaa tgcctaattc cgagaagcct gcggccagta cagcactacc acagccacca
     8401 aaaacaagcc agaaagtacc aaaacattca gctgctaatt ttctgaacat atccacctca
     8461 attaaaaatt gaccctgtga aaaatatggt cgttttatag ggtcgtcgta aaaaagtgac
     8521 gacggaaata atgcgcggct attttaaaaa cgaaggcgag tcattcacca gataaataaa
     8581 tccagtaaat ttgatttagg gcaacagcgg gttgccccat atagtcattt gtctgattga
     8641 cagtgtagtg caagcaaaag atttaatcct ttaggcgtaa taaaaaataa tttatcatgc
     8701 taattatttg attttgttgt ttttgcagac ttatcagcaa gagggagtat aacgcgatta
     8761 ttcgctcatt tttcagacat ttgccatgct taaatgtgat gtcatcacgt attagcaagg
     8821 cctttcccgt tatactgcca gcgtaaagga taagtcacat atttctggag gggatatgat
     8881 tcttgagcgc gttgaaattg tgggttttcg cggtatcaac cgtttgtcgt tgatgctcga
     8941 acaaaacaac gtcctgattg gggagaacgc gtggggtaaa tccagcttgc tggacgcctt
     9001 aaccctgctg ctatcgccag aatcagatct ctaccatttt gagcgcgacg atttctggtt
     9061 cccgccggga gatatcaacg ggcgagaaca tcatctgcat attattttga ccttccgcga
     9121 atcgctgcca ggccgacatc gggttcgccg ttatcggccg ctggaagcgt gctggacgcc
     9181 atgcaccgat ggctatcacc gtatttttta tcgtctggaa ggggagagtg cggaagacgg
     9241 cagcgtgatg acactgcgca gttttctcga taaagacgga catccgattg atgtcgagga
     9301 tattaacgat caggcacgcc atctggtgcg tttaatgccg gtgctgcgct tgcgtgatgc
     9361 ccgttttatg cgccgtattc gtaacggcac ggtgccaaat gttcccaatg tggaagtcac
     9421 cgcgcgccag ctcgatttcc tcgcccgtga gttgtcctca catccgcaaa atctctctga
     9481 tggacagatt cgtcagggac tttccgcaat ggtgcagttg cttgagcatt atttctctga
     9541 gcagggggct ggacaggcgc gatatcgttt aatgcggcgg cgggccagca atgagcaacg
     9601 aagctggcgc tatctggaca tcatcaaccg gatgattgac cgacctggag ggcgctcgta
     9661 tcgggttatt ttgctcggcc tgtttgctac tttattgcag gcaaaaggca cattgagact
     9721 ggataaagac gcgcgtccgt tattgctaat tgaagatcca gaaacccgcc tgcatccgat
     9781 tatgctttcc gttgcctggc atctgttgaa tcttttgcct ttacagcgta ttgctaccac
     9841 caactctggc gagctgcttt cgttaactcc agtggaacat gtttgccgcc tggtgcgtga
     9901 atcttctcgt gttgccgcct ggcgtctggg gccaagtggc ttgagcaccg aagacagccg
     9961 ccgcatctct tttcacattc gttttaatcg tccgtcatcg ctgtttgctc gctgctggtt
    10021 gctggtggaa ggggaaacgg aaacctgggt tatcaatgaa ctggcgcgtc agtgcggaca
    10081 tcattttgat gccgaaggga tcaaggtcat tgagtttgcc cagtccgggc taaagccact
    10141 ggttaaattt gcccgccgaa tggggattga atggcatgta ctggtcgatg gcgatgaagc
    10201 agggaagaaa tatgccgcta cggtacgcag cctgttgaat aacgatcggg aagccgaacg
    10261 agaacattta acggcgttac cggcgctgga tatggaacat tttatgtatc gccagggatt
    10321 ttccgatgtg ttccaccgcg tggcgcaaat cccggaaaat gtaccgatga atctgcgcaa
    10381 aattatctcg aaagcgatcc atcgctcttc caaacccgat cttgccattg aagtggcaat
    10441 ggaggccgga cgtcgtggtg tggactccgt accgacgctg ctgaaaaaaa tgttctcacg
    10501 cgtgctgtgg ctggcgcgcg gtcgcgcgga ttaaccgcga aacatcgtgg ccatttgtgg
    10561 ctgaatagcg tcgagcatct catagcgccg acggtattca gcccgttttt tactggcgat
    10621 ttcggcaatc tcttttcgtg ctatctgtgc tggaaggcgg taatggcgtt cagcatcaca
    10681 tacgccgcca accgattccc agaaagcgtt gtaatcggca tggatcttgc cttctttatc
    10741 acgataacgc aggctgcggt aaatatgcgt ttcattgctg acggcaataa tctgctctac
    10801 ctgcaaacgt tgggcaaaca gacaggccgc ttccatcacg aggcgtttgg gaaatagccc
    10861 gtggcaggct ttcgtcgcat tctggatttc ctgatgtgga atttcccatt ttgcgccttg
    10921 cagtccgcca ataaacatcg ttcttttccc ctgatattca cacagggtaa acgtgatctc
    10981 tgccagagga ataccttcgc tgttgcgaaa caggattgtg ctgtcacctt ctttatccat
    11041 tgagatcatc atggtcagct caagcgtgaa ctgctcgccg tttttgcctt ccagcttcgc
    11101 cagttgcagc ccgggggtat tcaaatataa gctgaattct tccgccgaca tacatccgcg
    11161 gagtaacgca taatggtaac gtaacgcctc cagcaattgc ttacggctaa gattcgccgc
    11221 aaggtaaggg cgatgcagac gcacaggcag tcgcggttgg cgcgttaaca acacattgag
    11281 attaggccag tgggaaagtt cgttcatcca ctcaacgctt aaacgcggta taatcaacga
    11341 gcgcagcaaa aatttctggc gaaaactacg gcgatgccag aatttacccg gccgacactg
    11401 tccacgtgcc agactaagaa aaagtgacag gctgctgaga gattcagatg gcgtaaaggt
    11461 ccgttcagtt agctgcgaca tattcatgaa atcaatggtt atacatgacg tcgatttcac
    11521 cattgcgtat cttaaccaaa catcaatagt gtgattacta acgtaaattt tatggttttg
    11581 ttgatatttc gttgaagtta atgacccgga ttggcatatg gagtattcag aatatttatg
    11641 aaaaagcgga aaaccgtgaa gaagcgttac gttattgcgc tggtgatagt catcgccgga
    11701 ctgattacgt tgtggagaat tcttaacgca cccgtgccga cttatcagac gctgattgtg
    11761 cgccccggtg atttacagca aagcgtgctg gcgaccggaa agctggacgc gctgcgtaag
    11821 gttgacgtgg gcgcgcaggt cagcggtcag ttgaaaactc tgtcggtggc gattggcgat
    11881 aaagtaaaaa aagaccagct tttaggggtt attgatcctg aacaggctga aaaccagatt
    11941 aaggaggtcg aagcaacgct gatggagcta cgtgcgcagc ggcagcaggc ggaagcggag
    12001 ctgaaactgg cgcgggtgac gtattcccgt cagcaacgtc tggcacaaac gcaggctgtt
    12061 tcacagcagg atctcgacac cgccgcgacg gagatggctg tgaaacaggc gcaaattggc
    12121 accattgacg cgcaaatcaa gcgcaatcag gcttctctcg atacggctaa aaccaatctc
    12181 gattacactc gcatcgttgc cccgatggcc ggggaagtca cgcaaatcac cactctgcaa
    12241 ggccagacgg tgattgccgc acaacaagca ccgaacattc tgacgctggc agatatgagc
    12301 accatgctgg taaaagcgca ggtttctgaa gcggatgtaa tccacctgaa gccggggcaa
    12361 aaagcctggt ttacggtgct tggcgatcca ctgacgcgct acgaggggca aatcaaggat
    12421 gtactaccga cgccggaaaa ggttaacgac gctattttct attacgcccg ttttgaagtc
    12481 cccaacccca atggtttgct gcggctggat atgactgcgc aagtgcatat tcagctcacc
    12541 gatgtgaaaa atgtgctgac gatccctctg tcggcgttag gcgatccggt tggcgataat
    12601 cgttataaag tcaaattgtt gcgtaatggt gaaacacgcg agcgtgaagt gacgattggc
    12661 gcacgtaacg ataccgatgt tgagattgtc aaagggcttg aagcgggcga tgaagtggtg
    12721 attggtgagg ccaaaccagg agctgcacaa tgacgccttt gctcgaatta aaggatattc
    12781 gtcgcagcta tcctgccggt gatgagcagg ttgaggtgct gaagggcatc agcctcgata
    12841 tttatgcggg tgagatggtc gcgattgttg gcgcttcggg ttccggtaaa tcgaccctga
    12901 tgaatattct cggctgtctg gataaggcca ccagcggcac ctatcgcgtc gccggtcagg
    12961 atgttgccac gctggacgcc gatgcgctgg cgcaactgcg ccgcgagcat ttcggcttta
    13021 ttttccagcg ttaccatttg ctttcgcatt taaccgccga gcagaacgtt gaagtacccg
    13081 ccgtctatgc tggtcttgag cggaaacagc gactgcttcg tgcccaggag ttgctgcaac
    13141 ggctggggct ggaagaccgt acagagtatt atccggcaca gctttcgggt ggtcagcaac
    13201 agcgcgtcag catcgcgcgg gcattgatga acggtggtca ggtaattctt gccgatgaac
    13261 caaccggcgc actggacagc cattctggcg aagaggtgat ggcgatcctg catcagctgc
    13321 gcgatcgtgg gcatacggtg attatcgtca cccacgatcc gcaggtcgct gctcaggccg
    13381 agcgggtgat cgaaattcgc gacggcgaaa ttgtgcgcaa tcctcccgcc attgaaaaag
    13441 tgaatgttgc tggcgggacg gagcctgttg tcaacacggt gtctggctgg cggcagtttg
    13501 tcagcggttt taacgaggcg ctgacgatgg catggcgggc actggcagcg aataaaatgc
    13561 gtactttact gaccatgctg gggattatta tcggtattgc gtcggtggtt tccattgtcg
    13621 tggtgggtga cgccgccaaa caaatggtgc tggcggatat tcgttctata ggtacgaata
    13681 ctattgatgt ctatcccggg aaagattttg gcgatgacga tccgcaatat cagcaggcgc
    13741 tgaagtacga cgacttaatc gccatccaaa aacaaccgtg ggtcgcctca gccacacctg
    13801 ccgtctcgca aaacctgcgc ctgcgttata acaatgttga tgttgctgcc agtgccaatg
    13861 gcgtgagcgg cgattatttt aatgtctatg gcatgacctt cagtgaagga aacaccttta
    13921 atcaggagca gctgaacggt cgtgcgcagg tcgtggttct cgacagtaat actcgccgcc
    13981 agcttttccc ccataaagca gatgtggttg gcgaggtgat tctggtcggc aatatgcccg
    14041 ccagagtcat tggtgtggcg gaagaaaaac agtcgatgtt tggtagcagt aaagtgctgc
    14101 gtgtctggct accttacagc acgatgtccg ggcgagttat gggccagtcg tggcttaact
    14161 ccattactgt cagggtaaaa gaaggatttg acagcgccga ggcggaacag caactcacgc
    14221 gtttactttc actgcgccac ggtaagaagg atttctttac ctggaacatg gacggcgtct
    14281 tgaaaactgt tgaaaagacc acacgcactt tacaactgtt tctgacgctg gtggcggtga
    14341 tttcgctggt ggtgggtggt attggtgtaa tgaatattat gctggtgtca gtgaccgagc
    14401 ggacgcggga aattggcatt cgcatggctg taggtgcgcg agcaagcgat gttttgcaac
    14461 agttcctgat cgaagccgta ctggtttgcc tggtcggtgg cgcgttggga ataacactgt
    14521 cactgttaat tgctttcacc ttgcagcttt tcttacccgg ctgggagatt ggtttttcac
    14581 cgttggcact gctgctggcg tttctctgct cgacggccac cgggatttta tttggctggt
    14641 tacccgcacg aaatgcggca cgactggatc cagtagatgc tctggcacga gagtaatttt
    14701 tgagataaaa atgccagccg atcgggctgg cattttgcct ttaggatgta cacaatgaga
    14761 cagaagagct atgcgactgc cgcttctact tcgacgggca caataacact ggcgtgattg
    14821 ccttttggcc cctggtggac atcaaactga acggattgtc cagcttttag cgttctgtaa
    14881 ccatccatct gaatggtgga ataatgagcg aaaatatctt cgccgccgcc ttcagggcag
    14941 atgaaaccaa accctttggc attgttgaac cacttaacag tacccttttc catgcttcga
    15001 catccttcgc aaatcttata caagtaagat ggaataaacc ggggtcagag agggggctgt
    15061 tcaaaacctc gccaactcta gaaatacaat ttagagaatt agggcgagcc gtcaagcatt
    15121 tgacagggga cagggggcag gtatgaatca aaaatttgaa gcagttaacg ctattgacag
    15181 gaatgtgaca gatgtcgctg atgccaacga tagatgatag ttatctatca tgtgaagtag
    15241 attggtcagg caaataagct cttgtcagcg gcagggcgtt ctgccgataa ccgtaaccga
    15301 agatgataac tgacaatggg taaaacgaac gactggctgg actttgatca actggcggaa
    15361 gaaaaagttc gcgacgcgct aaaaccgcca tctatgtata aagtgatatt agtcaatgat
    15421 gattacactc cgatggagtt tgttattgac gtgttacaaa aattcttttc ttatgatgta
    15481 gaacgtgcaa cgcaattgat gctcgctgtt cactaccagg ggaaggccat ttgcggagtc
    15541 tttaccgccg aggttgcaga aaccaaagtg gcgatggtga acaagtacgc gagggagaat
    15601 gagcatccat tgctgtgtac gctagaaaaa gcctgaatgc aggcataaaa attgggggag
    15661 gtgcctatgc tcaatcaaga actggaactc agtttaaata tggctttcgc cagagcgcgc
    15721 gagcaccgtc atgagtttat gaccgtcgag cacttgttac tggcgctgct cagtaaccca
    15781 tctgcccggg aggcgctgga agcgtgttct gtggatttgg ttgcgctccg tcaggaactg
    15841 gaagccttta ttgaacaaac cacacccgtt ctgcctgcca gtgaagagga gcgcgacaca
    15901 cagccgacgc tgagttttca gcgtgtactg caacgtgcgg tcttccatgt ccagtcctcc
    15961 ggtcgcaatg aggtaaccgg tgcaaacgtt ctggtcgcta tctttagcga acaggagtcg
    16021 caggcggcat atctgttgcg taaacatgaa gtcagccgtc tcgatgtggt gaactttatc
    16081 tctcatggca cgcgtaaaga cgagccgaca cagtcttctg atcctggcag ccagccaaac
    16141 agcgaagaac aagctggtgg ggaggaacgt atggagaatt tcacgacgaa cctgaatcag
    16201 cttgcgcgcg tgggcggaat cgacccactg attggtcgtg agaaggagct ggagcgtgct
    16261 attcaggttc tctgccgtcg ccgtaaaaac aacccgctgc tggtggggga atctggtgtc
    16321 ggtaaaaccg cgattgcgga aggtcttgcc tggcgaattg ttcagggcga tgtgccggaa
    16381 gtgatggctg actgtacgat ttactctctc gatatcggtt ctctgttagc gggcactaaa
    16441 tatcgcggcg actttgaaaa acgttttaaa gcgttgctca agcagctgga gcaggacact
    16501 aacagcatcc tgtttattga tgagatccac accattatcg gtgcgggtgc agcgtctggt
    16561 ggccaggttg atgcggctaa cctgatcaaa ccgttgctct ccagcggtaa aattcgcgta
    16621 attggttcga caacctatca ggagttcagc aacattttcg agaaagaccg tgctctggcg
    16681 cgtcgcttcc agaaaattga tattactgaa ccgtcgatcg aagaaactgt tcaaatcatc
    16741 aatggcctga aaccgaagta tgaagcgcac cacgacgtgc gttataccgc aaaagcggtg
    16801 cgtgcagcgg tagagctggc ggtgaaatac attaacgatc gtcatctgcc ggataaagcc
    16861 attgacgtta tcgacgaagc gggcgctcgc gcacgcctga tgccggtaag caaacgcaag
    16921 aaaaccgtta atgtggcgga tattgagtcc gtggtggccc gtattgcgcg cattccagag
    16981 aagagtgttt ctcagagtga ccgcgatacc ctgaaaaacc tcggcgatcg cctgaaaatg
    17041 ctggtcttcg gtcaggataa agccattgag gcgctgactg aagccattaa gatggcgcgt
    17101 gcaggtttag gtcacgaaca taaaccggtt ggttcgttcc tgtttgccgg ccctaccggg
    17161 gtcgggaaaa cagaggtgac ggtacagctt tcgaaagcgt tgggcattga gctgctgcgc
    17221 tttgatatgt ccgagtatat ggaacgccat accgtcagcc gtctgattgg tgcgcctccg
    17281 ggatacgttg gttttgatca gggcggtttg ctgactgatg cggtcatcaa gcatccacat
    17341 gcggttctgc tgctggacga aatcgagaaa gcgcaccctg acgtgttcaa tattctgttg
    17401 caggtgatgg acaacggtac gctgaccgat aacaacggac gcaaagcgga cttccgtaac
    17461 gtggtgctgg tgatgaccac caacgccggg gtacgtgaaa ctgagcgtaa atccattggt
    17521 cttatccacc aggataacag caccgatgcg atggaagaga tcaagaagat ctttacaccg
    17581 gaattccgta accgtctcga caacattatc tggtttgatc atctgtcaac cgacgtgatc
    17641 catcaggtgg tggataaatt catcgtcgag ttgcaggttc agctggatca gaaaggtgtt
    17701 tctctggaag tgagccagga agcgcgtaac tggctggccg agaaaggtta cgaccgggca
    17761 atgggcgcac gtccgatggc gcgtgtcatc caggacaacc tgaaaaaacc gctcgccaac
    17821 gaactgctgt ttggttcgct ggtggacggc ggtcaggtca ccgtcgcgct ggataaagag
    17881 aaaaatgagc tgacttacgg attccagagt gcacaaaagc acaaggcgga agcagcgcat
    17941 taatctgatt gttaggtagg ttggtcaagt ccgtaatctc gaaagaggtt acggactttt
    18001 tgtttatggg gtggaggagg ttcagaccct ttttttaatg atgatggtaa gttgttgata
    18061 attagtgctg cgggaaggta aggataaaaa agggtgctgc aggagaatgg gatggttttg
    18121 ctttattaac aacgggctaa acgtgtagta tttgagttca ctgccgtaca ggcagcttag
    18181 aaatcgacgg ggtgcggtaa aacctttgcg aacgcgttca ctgccgtaca gacagataaa
    18241 atgcgaaaaa aaagctcgca ctttcgtacg agctcttctt taaatatggc ggtgaggggg
    18301 ggattcgaac ccccgatacg ttgccgtata cacactttcc aggcgtgctc cttcagccac
    18361 tcggacacct caccaaattg ttttgctacc aaacctcatg ggtggcaacg gggcgctact
    18421 atagggagtt ggagtaaaac ggtcaagaag aattttaatg ataattattg tttgctcata
    18481 ctgtaaacaa gttgtgcagt atatctacat cgagacaagt tacggactta tacttccaaa
    18541 gtacttcata catatcacaa aataaaaagg ccggttaaac cgacctttta ctcgttcttt
    18601 ctcttcgccc atcaggcggt aaaacaatca gcgactacgg aagacaatgc ggcctttgct
    18661 caggtcgtac ggggtcagtt caacagtcac tttgtcgccc gtcaggatgc ggatgtagtt
    18721 tttgcgcatt ttaccggaga tgtgtgcagt aaccacgtga ccgttttcta actctacgcg
    18781 gaacatggta ttaggcaacg tttcaagaac ggtaccttgc atttcaatat tgtcttcttt
    18841 ggccatctaa tcctctgggg tatcactacc gtaatttgaa ccggcaagat aatgccgaag
    18901 ttctgtaaat aagtaaagat ttgcgcgcta aatcgcaaca aacaggttcg gcacattact
    18961 ccgaaaacac acggctaagc cgcaccaaaa gcgcaacgta taagggagcg gtgagataaa
    19021 cgatgggcgt tacctgacgc gaaaaattcc ttatcggcag cggggtaatg agcgtaacca
    19081 actctgcgac cgcaattata acactctggg gagaaatgtg ccgaaaacat tcattcttgt
    19141 ggtgaaaaca agcatcgtgg tacccagaaa ttattcggca atcgtccgag gcgcatttga
    19201 ttgagataat taaggtaatc ccggcgggga atttcgcagg caccaagcga tgctgtgtga
    19261 tcgttaagga cctggcagtc gataagctta ccgccatgac cgataaattc ctcacagaat
    19321 accagaagcg ccgttttaga cgcattttcc atccggctga acatggactc gccacaaaat
    19381 agcgttccct gggccacgcc gtacataccg ccgacaagct catcttcacg ccagacttca
    19441 atggagtggg catggccgag ttcgtgaagg cgatggtagg cttcgaccac gccacgcgtg
    19501 atccaggttc cttcttcgcg atcgctggca cagccttcaa tgacctgacc aaaagcgtaa
    19561 ttcatcgtga cacgataggg cgagcgttta tgaaatcgct tcatactacg gctgatatgc
    19621 agtgattctg gccatagcac cgcgcgggga tcgggcgacc accagaggat ggggtcgcct
    19681 ggagaaaacc acggaaaaat accacgctgg taagccatta acaggcgcgc agggctaaga
    19741 tcgcccccaa gtgccagcag gccgttaggc tcacgtaatg cgccttccgg ggaagggaag
    19801 gctattgaat ggcgagaaag ctgaaccagg cgcatgaccg caaaactcca cgcaagtcgg
    19861 atcgttcaat aatagcttac aaaccctgct tgaactggta ataacgcccc tgtctggcaa
    19921 gcagctctgc gtgagtacct tgctcaataa tttgcccgtt gtccatcact attatttgtt
    19981 ggaaacgaga gagtccgcga agtcgatggg tgaccattaa caccgttttc tcactcatca
    20041 tttctgcaag caattcaagg atctggcttt cggttgtggc atctaagcct tcggtaggtt
    20101 catccagcaa caccagtggc gcatcatgta acagcgcacg ggcgatagcc agacggcgca
    20161 gttcaccacc ggagagctgg cgtccgcctt cacctaacca actgttgaga cctgcatcct
    20221 cgagcagctt ctccagacca acgcgacgca agatctccga cagagcctca tcactactgc
    20281 caggcgaggc gagtaacaga ttatcacgca gcgtggcgct aaacagatgc actcgctgag
    20341 gaacaacgct gatggtctgt cgtagagccg cttcattcag gctggctatg gggctatcgt
    20401 taagcaaaat ctcgccctgt tgcgggtccc atgcgcgggt cagcagttgt aacagtgttg
    20461 atttgccgca tccggttcgc ccgagaatcg ctatatgttc cccggcgttt acctgaagag
    20521 aaatcccttt aagtgcctgt tgggattgct ccggataagt gaactgaaca tcccgtaatg
    20581 tcagcgaaac gcgatcggca acacgagttt ggggatcagg gaaggtgacc tccggttttt
    20641 gatccgttaa gtcagagata cgtacggcag aggcaatgac ttgccccaga tgctgaaatg
    20701 cacccgttac tggtgccagt gcttcaaacg cggctaacgc gcagaagaca aacagggcaa
    20761 ttaacgcgcc gggttgagca ttgccgccaa cgccgccaga cgccatccac agcatcagga
    20821 tcaccgctaa cgcgccaatg agcagcatta tcgcttgcga caatgcggtc agttcagatt
    20881 gacggcgttg cgcttccagc cattgaattt ctgtattctc tagttgcgtg cgataacgat
    20941 cgctggcacc aaaaatggtc agctcagctt gcccttgcag ccaggccgtc agttgttggc
    21001 gatactgccc gcgaagatga gtcagatttt gcccggtgct ttttcccgca cgataaaaca
    21061 gcggtggcat caggaaaagc gtcagtaaca taatgccgcc aagcgtaaag gcgagggtga
    21121 aatcaaggaa acttaacccg attgtcacca ccataatcac cacaaaagcg cccaccagcg
    21181 gcgagataac gcgcaggtaa agatgatcga gcgtatcaac atccgccacc acgcggttga
    21241 gcaattcgcc ctgacgatag cgtgccagtc cggcagggga gaggggcagc aatttgctga
    21301 aggtgtaaat gcgcagatgc tgcaacacgc ggaaagtcgc gtcgtgactt accagacgtt
    21361 caaaataacg cccggcagta cgggtgattg ctgcgccacg cacgcccgca gcgggtagca
    21421 tatagttgaa gctgtacagt ccggcaaccc ccgcaaccgc tgaggccgag aggaaccagc
    21481 cggaaagtgt caacagaccg atactggcga gcagcgtgac aattgccagc acgataccaa
    21541 gacttaacat ccatttatga cgtttataca gtgccagata gggtagcaaa gcgcgcattt
    21601 aaatctcctc ctgacgatgg gccagtaatg tggcgaatgg gccaccagcc acacttaatt
    21661 ccgcgtaacg tccttgctca ataatctgac catcctgcat tacccaaatg acatcccagt
    21721 cagcaagatc ttctaactgg tgggtgacca ttaacgttgt ctggcgcaga gaggcggcat
    21781 tcagcgcctc cattacgcgc tgttcactgt gagcatcaag gctggcagcg ggttcatcca
    21841 atagtaatag cgagcaggga tttagtaacg cacgggccac cgccacgcgc tgcgcctgcc
    21901 ccacggaaag gcgggcagcc tggtcgccaa caggcgtatc aacgccttgt ggcaggagcg
    21961 gtagaaactc gctgacccag gcgttatcca gcgctgcttg taattcatgt tcgctggcat
    22021 caggtcgcgc cagtagtacg ttatcccgta atgttgctgc cggtaattgt ggattttgcc
    22081 caacccagga gagatgttta cgccatgatt ctggtgataa atcgcgtaat tctatcccgt
    22141 tgattcgtag cgatccctga tatgagagaa aaccagaaag cgcgttcaac agtgaacttt
    22201 tacctgaacc gctgcgacca accaacacgg cacgttggcc tgctggcaaa gtaaagttca
    22261 gcggtccggc cagcgtttta ccttccggcg acgtgataaa cagatcctcg gcctcaatgg
    22321 tcaccggatc ggtcgatgct aattccacct cgccacgctg cggatgggcg agcggggttt
    22381 ccataaacgt tttcagactg tcagctgcac caacagcctg ggctttagca tgataaaacg
    22441 taccgagatc gcgtaatggc tggaaaaact ctggcgcaag gatcagggcc agaaaacccg
    22501 cagccagcgt cacgccagta tcgtagtgac caaaatccag ctcgccgaga taggaaaaac
    22561 caaagtagac cgccaccaga gcaattgaca gcgaggtaaa aaattcgaga atgccggagg
    22621 ataaaaatgc cagccgtagc acttccattg tccgttggcg gaaatcttcc gaagcagaac
    22681 gaatactttc aatttcagct tcaccacgac caaaaatacg caatgtttcc atgccgcgca
    22741 ggcgatcgag gaaatgccca cttaagcgag caagagcgag aaagttacgt cggttagcat
    22801 cggcagcccc catcccaacc agcgccataa acaacggaat taacggtgca gtgcccagca
    22861 gaatgagcgc cgcagcccag ttagagggga agatcgccac cacaatcagc aacggcaccg
    22921 acactgccag cgccatttgc ggcaggtagc gtgcatagta atcatgcata tcgtcaattt
    22981 gctcgagtac cagcgtcgcc cagctccccg caggtttacc ctgaatccac gctggccctg
    23041 cttgttgcag acggtcgaga acctgacggc ggatggcaaa gcggatatgc tgcccggcgt
    23101 gataacccac ccgttcgcgt aaccagacca cccatgcgcg cagtacaaag gtcagaacca
    23161 gtaacgtaaa gggaagcagc agagcttcac ggggaatatt ctccataatc atatgttgca
    23221 gaattcgcgc catgaaccag gcctgggcaa tgatcaatat gccgctcaca aagcccagca
    23281 gacgagaaat attcagccaa cgttgggaga tgacgctttg ctgttttaac cagcgggtta
    23341 actctttttg acgggattta ttcattgcac gcttagcagg tgagttatca gaattatttg
    23401 tagagcaatg ttacaacgag gaaaaaataa aggcgaccca tagtcgcatg gtgtcgcctt
    23461 ctttactttt gttactgatt tgtaaaatta ttttgcgtca gctaaaccat cgaggtagcg
    23521 ttccgcatca agtgctgcca tgcagcctgt accagcagat gtaatagcct ggcgataaat
    23581 gtgatccatc acgtcgcctg cggcaaagac gccagggatg ctggtctggg tggcattacc
    23641 atgaataccc gactgtactt tgatgtagcc gttttccagt tccagctgcc cttcgaaaat
    23701 ggcagtattc gggctgtgac cgatagcaac aaacagaccg gcaacgtcga gtgactcgat
    23761 gttatcgctg ttttgcgtat cgcgcagacg aacgccagtg acgcccatct gatcgccggt
    23821 cacctcttcc agcgtacggt tggtgtgtag aatgatgttg ccgttctcca ctttatccat
    23881 cagacgctta ataaggattt tttccgcgcg gaaaccgtca cggcggtgaa tcagatgcac
    23941 ttccgaagcg atgttagaca gatacagcgc ctcttcaacc gcggtattgc cgccgccgat
    24001 gaccgcaact ttctggttgc gatagaagaa accgtcgcag gtagcacaag cagaaactcc
    24061 acggccttta aaggcttctt cagatggcag gccgagatag cgtgcagaag ctccagtggc
    24121 aataatcagc gcgtcgcaag tgtattcgcc gctatcgcca gtcagacgga acggtcgatt
    24181 ctgcaaatca actttgttga tgtgatcaaa gatgatttca gtttcaaatt tggtggcatg
    24241 ttcgtgcatg cgctccatta ataacggacc ggtcagatcg tttggatcgc caggccagtt
    24301 ttccacttcc gtggtggtgg tcagttggcc gcctttttcc atgccggtaa tcagcacagg
    24361 ttgcaggttg gcgcgcgctg catagacagc agcggtgtat cccgccgggc ctgaacccag
    24421 gataagcagt ttactgtgtt tggtcgtgcc catgagatcc ccatagttgt tggcagacaa
    24481 tgggcaggat tgtagggaat ttatagacgt aaaaaaagag tatgacgatt ttgttaacaa
    24541 tttgtgcaat cggcagcatc gataagcagg tcaaattctc ccgtcattat cacctctgct
    24601 acttaaattt cccgctttat aagccgatta aatgatgaat aaacgcccct gttaatgaat
    24661 atctggcatg ttgtactaaa aatcgatgtt ttgctttgac aatcccctgg tgttttgcga
    24721 aaacattcga ggaagaaaaa aaacagtatt cttatatgcg cataaccatg catgtaaata
    24781 ccatgtttac cgtgctagtg aaatctacgt atggcgtgga cagacgccat tcgtgatgtc
    24841 gatagctgcc acaaggcaac ggtcttctca ccgtagaccc aggcattgcg cgccgtgaat
    24901 cttcatgatt tcggtctatc gtgacgggta gcgactctga acagtgatgt ttcagagtca
    24961 gacaggagta gggaaggaat acagagagac aataataatg gtagatagca agaagcgccc
    25021 tggcaaagat ctcgaccgta tcgatcgtaa cattcttaat gagttgcaaa aggatgggcg
    25081 tatttctaac gtcgagcttt ctaaacgtgt gggactttcc ccaacgccgt gccttgagcg
    25141 tgtgcgtcgg ctggaaagac aagggtttat tcagggctat acggcgctgc tgaaccccca
    25201 ttatctggat gcatcacttc tggtattcgt tgagattact ctgaatcgtg gcgcaccgga
    25261 tgtgtttgaa caattcaata ccgctgtaca aaaacttgaa gaaattcagg agtgtcattt
    25321 agtatccggt gatttcgact acctgttgaa aacacgcgtg ccggatatgt cagcttaccg
    25381 taagttgctg ggggaaaccc tgctgcgtct gcctggcgtc aatgacacac ggacatacgt
    25441 tgttatggaa gaagtcaagc agagtaatcg tctggttatt aagacgcgct aacacggaac
    25501 aggtgcaaaa tcggcgtatt ttgattacac tcctgttaat ccatacagca acagtactgg
    25561 ggtaacctgg tactgttgtc cgttttagca tcgggcagga aaagcctgta acctggagag
    25621 cctttcttga gccaggaata cactgaagac aaagaagtca cattgacaaa gttaagtagc
    25681 ggccgccgcc ttctggaggc gttgctgatc cttattgtcc tgtttgccgt ctggttgatg
    25741 gctgccttac taagctttaa cccttcggac cccagctggt cgcaaacggc ctggcatgaa
    25801 cctatccata atttaggtgg gatgcccggt gcgtggctgg cagatacgct gttctttatt
    25861 tttggcgtga tggcttacac cattcccgtc attattgtcg gcggttgttg gtttgcctgg
    25921 cgtcatcagt ccagcgacga atacattgat tattttgccg tttcgctacg catcattggc
    25981 gttttggcgc tcatccttac ctcctgtggt ctggcggcaa tcaacgctga cgatatctgg
    26041 tattttgcct ccggtggcgt cattggcagc ttactaagta ctacgctaca accactgcta
    26101 cacagtagcg ggggaactat tgcgctgctc tgcgtttggg cagcgggcct gacgctgttc
    26161 accggttggt catgggtgac cattgctgaa aaactcggcg gctggatttt aaacattctc
    26221 accttcgcca gtaatcgtac ccgtcgcgat gatacctggg tcgatgaaga tgaatatgaa
    26281 gatgacgaag agtatgaaga tgaaaaccac ggcaaacagc atgaatcacg ccgtgctcgt
    26341 attcttcgcg gcgcgctagc gcgtcgtaaa cggttggcgg aaaaattcat taatccgatg
    26401 gggcggcaaa cagacgctgc gttgttctcc ggtaagcgga tggatgatga cgaagagatt
    26461 acctacactg cacgcggtgt ggctgccgac cctgacgacg tcctattttc gggcaatcgt
    26521 gcaacgcagc cagaatatga cgaatacgat ccattattaa acggtgcgcc aattaccgaa
    26581 cctgtcgctg tagcagctgc tgctaccacg gcgacacaaa gctgggctgc gccggttgaa
    26641 cctgtgactc agacgccgcc tgttgcctct gttgacgttc caccttcgca acctacagta
    26701 gcctggcagc ctgtaccggg tccacaaacg ggagagccgg ttattgctcc tgcaccggaa
    26761 ggttacccac agcagccaca atatgcgcag cctgcagtgc aatataatga gccgctgcaa
    26821 caaccagtac agccacagca gccgtattat gcacctgcag ctgaacaacc tgcgcaacag
    26881 ccgtattatg cccctgcgcc agaacaaccg gtggcaggta acgcctggca agccgaagag
    26941 cagcaatcca cttttgctcc acagtctaca taccagactg agcaaactta tcagcagcca
    27001 gccgctcagg agccgttgta ccaacagccg caacccgttg aacagcagcc tgttgtggag
    27061 cctgaacccg ttgtagaaga gacaaaaccc gcgcgtccgc cgctttacta ttttgaagaa
    27121 gtggaagaga agcgagcccg tgaacgtgaa caacttgcgg cctggtatca accgattcca
    27181 gaaccggtta aagaaccaga accgatcaaa tcttcgttga aagcgccttc tgttgcagca
    27241 gtacctccag tagaagccgc tgccgctgtt tctccgctgg catctggcgt gaaaaaagcg
    27301 acactggcga cgggggctgc cgcaaccgtt gccgcgccag tcttcagtct ggcaaatagc
    27361 ggtggaccgc gtccgcaagt caaagagggg attggcccgc agttgccacg accgaaacgt
    27421 atccgcgtgc caactcgtcg tgaactggcg tcttacggta ttaagctgcc ctcacagcgt
    27481 gcggcggaag agaaagcccg tgaagcccag cgcaatcagt atgattctgg cgatcagtat
    27541 aacgatgatg aaatcgatgc gatgcagcag gatgaactgg cacgtcagtt cgcccagaca
    27601 cagcagcaac gctatggcga acagtatcaa catgatgtgc ccgtaaacgc agaagatgca
    27661 gatgctgcgg cagaggctga actggctcgt cagtttgcgc aaactcaaca acaacgttat
    27721 tccggcgaac aaccggctgg ggcgaatccg ttctcgctgg atgattttga attttcgcca
    27781 atgaaagcgt tgctggatga tggtccacac gaaccgttgt ttacgccaat tgttgaacct
    27841 gtacagcagc cgcaacaacc ggttgcaccg cagcagcagt atcagcagcc acaacagccg
    27901 gttgcgccac agcagcaata tcagcagccg caacagccgg ttgcgccgca gccgcagtat
    27961 cagcagccac aacagcaggt tgcgccgcag ccacaatatc agcagccgca acaaccggtt
    28021 gcgccacagc cgcaatatca gcagccgcaa caaccagttg cgccgcagca gcagtatcag
    28081 cagccgcaac aaccggttgc accgcagccg cagtatcaac agccgcaaca accggttgca
    28141 ccgcagcagc aggataccct gcttcatccg ctgttgatgc gtaatggcga cagccgtccg
    28201 ttgcataaac cgacgacgcc gctgccttct ctggatttgc tgacaccgcc gccgagcgaa
    28261 gtggagccgg tagatacctt tgcgcttgaa caaatggctc gcctggtgga agcgcgtctg
    28321 gctgatttcc gtattaaagc cgatgtcgtc aattactctc cggggccggt tatcactcgc
    28381 tttgaactta acctggctcc tggcgtaaaa gcggcgcgca tttctaactt gtcacgggac
    28441 cttgcccgtt cactttcgac ggtggcggtg cgtgtcgttg aagttattcc tggcaaaccc
    28501 tatgtaggtc tggagttacc aaataaaaaa cgacaaaccg tttatctgcg cgaagttttg
    28561 gataacgcca aattccgcga taatccgtcg ccattaaccg tggtgctggg taaagatatc
    28621 gccggtgagc cggtggttgc cgatctggcg aaaatgccgc acttgttggt tgcggggact
    28681 accggttccg gtaaatctgt cggtgtgaac gcgatgatcc tgagcatgct ttataaagca
    28741 cagccagaag atgtgcgttt catcatgatc gacccgaaaa tgctggagct ttcggtttat
    28801 gaaggcattc cgcatctgtt aacggaagtc gttactgata tgaaagatgc cgccaacgcg
    28861 ctgcgctggt gtgttaacga gatggagcgt cgttataaac tgatgtctgc gctgggtgtg
    28921 cgtaatctgg cgggttataa cgaaaaaatt gctgaagccg atcgcatgat gcgtccgatt
    28981 ccagacccgt actggaagcc gggtgacagt atggatgccc agcatccggt gctgaaaaaa
    29041 gaaccataca ttgtggtgtt ggttgacgaa tttgccgacc tgatgatgac ggtaggtaaa
    29101 aaagtggaag agctgatagc acgtctggcg caaaaagccc gtgccgcggg tatccacctc
    29161 gtactggcaa ctcagcgtcc atcggttgat gttattactg gtctgattaa agcgaatatt
    29221 ccgacccgta tcgcctttac cgtatccagt aagattgact cacgtaccat tcttgatcag
    29281 gctggcgcgg aatcactgct ggggatgggg gatatgctct actctgggcc gaactccacg
    29341 ttgccggtac gtgtccatgg tgcttttgtt cgcgatcagg aagttcatgc cgtggtgcag
    29401 gactggaaag cgcgtggtcg cccacagtat gttgatggca tcacttccga cactgaaagc
    29461 gaaggtggcg cgggcggttt cgatggcgct gaagaactgg atccgttgtt cgatcaggcg
    29521 gtgcagtttg tcactgaaaa acgcaaagcg tcaatttctg gcgtacagcg tcagttccgc
    29581 attggttata accgtgcagc gcgtattatc gaacagatgg aagcgcaggg gattgtcagc
    29641 gaacaggggc acaacggtaa tcgtgaagtg ctggccccac cgccgtttga ctaattaatg
    29701 cattgccgga taaggcgcgg tagcgtcgca tccggcactc tatcaactga aaattcagta
    29761 ttttcttctt tcctcaagct gattattagc ctggaataga gagtagaggg aactcccgat
    29821 cgggagtgac gtaatttgag gaataatgat gaaaaaaatt gccatcacct gtgcattact
    29881 ctcaagctta gtagcaagca gcgtttgggc tgatgccgca agcgatctga aaagccgcct
    29941 ggataaagtc agcagcttcc acgccagctt cacacaaaaa gtgactgacg gtagcggcgc
    30001 ggcggtgcag gaaggtcagg gcgatctgtg ggtgaaacgt ccaaacttat tcaactggca
    30061 tatgacacaa cctgatgaaa gcattctggt ttctgacggt aaaacactgt ggttctataa
    30121 cccgttcgtt gagcaagcta cggcaacctg gctgaaagat gccaccggta atacgccgtt
    30181 tatgctgatt gcccgcaacc agtccagcga ctggcagcag tacaatatca aacagaatgg
    30241 cgatgacttt gtcctgacgc cgaaagccag caatggcaat ctgaagcagt tcaccattaa
    30301 cgtgggacgt gatggcacaa tccatcagtt tagcgcggtg gagcaggacg atcagcgcag
    30361 cagttatcaa ctgaaatccc agcaaaatgg ggctgtggat gcagcgaaat ttaccttcac
    30421 cccgccgcaa ggcgtcacgg tagatgatca acgtaagtag aggcacctga gtgagcaatc
    30481 tgtcgctcga tttttcggat aatacttttc aacctctggc cgcgcgtatg cggccagaaa
    30541 atttagcaca gtatatcggc cagcaacatt tgctggctgc ggggaagccg ttgccgcgcg
    30601 ctatcgaagc cgggcattta cattctatga tcctctgggg gccaccgggt accggcaaaa
    30661 caaccctcgc tgaagtgatt gcccgctatg cgaacgctga tgtggaacgt atttctgccg
    30721 tcacctctgg cgtgaaagag attcgcgagg cgatcgagcg cgcccggcaa aaccgcaatg
    30781 caggtcgccg cactattctt tttgttgacg aagtgcaccg attcaacaaa agccagcagg
    30841 atgcatttct gccacatatt gaagacggca ccatcacttt tattggcgca accactgaaa
    30901 acccgtcgtt tgagcttaat tcggcactgc tttcccgtgc ccgtgtctat ctgttgaaat
    30961 ccctgggtac agaggatatt gagcaagtac taactcaggc gatggaagac aagacccgtg
    31021 gttatggtgg tcaggatatt gttctgccag atgaaacacg acgcgccatt gctgaactgg
    31081 tgaatggcga cgcgcgccgg gcgttaaata cgctggaaat gatggcggat atggccgaag
    31141 tcaatgatag cggtaagcgg gtcctgaagc ctgaattatt gaccgaaatc gccggtgagc
    31201 gaagcgcccg ctttgataac aaaggcgatc gcttttacga tctcatttcc gcactgcata
    31261 agtcggtacg tggtagcgca cccgatgcgg cgctctactg gtatgcgcga attattaccg
    31321 ctggtggcga tccgttatat gtcgcgcgtc gttgcctggc aattgcttcc gaagatgtcg
    31381 gcaatgccga cccacgggcg atgcaggtgg caattgcggc ctgggattgc tttactcgcg
    31441 ttggcccggc ggaaggtgaa cgcgccattg ctcaggcgat tgtttacctg gcctgcgcgc
    31501 caaaaagcaa cgctgtctac actgcgttta aagctgcgct ggcggatgct cgcgaacgtc
    31561 cggattatga cgtgccggtt catttgcgta atgcgccgac gaaattaatg aaggaaatgg
    31621 gctacgggca ggaatatcgt tacgctcatg atgaagcaaa cgcttatgct gccggtgagg
    31681 tttacttccc gccggaaata gcacaaacac gctattattt cccgacaaac aggggccttg
    31741 aaggcaagat tggcgaaaag ctcgcctggc tggctgaaca ggatcaaaat agccccataa
    31801 aacgctaccg ttaatgttat cgttgcggta atgttgttac tgtatccctg tggtcgcagg
    31861 ctgtggccac atctcccatt taattcgata agcacaggat aagcatgctc gatcccaatc
    31921 tgctgcgtaa tgagccagac gcagtcgctg aaaaactggc acgccggggc tttaagctgg
    31981 atgtagataa gctgggcgct cttgaagagc gtcgtaaagt attgcaggtc aaaacggaaa
    32041 acctgcaagc ggagcgtaac tcccgatcga aatccattgg ccaggcgaaa gcgcgcgggg
    32101 aagatatcga gcctttacgt ctggaagtga acaaactggg cgaagagctg gatgcagcaa
    32161 aagccgagct ggatgcttta caggctgaaa ttcgcgatat cgcgctgacc atccctaacc
    32221 tgcctgcaga tgaagtgccg gtaggtaaag acgaaaatga caacgtcgaa gtcagccgct
    32281 ggggcacccc gcgtgagttt gactttgaag ttcgtgacca tgtgacgctg ggtgaaatgc
    32341 actctggcct cgactttgca gctgcagtta agctgactgg ttcccgcttt gtggtaatga
    32401 aagggcagat tgctcgcatg caccgcgcac tgtcgcagtt tatgctggat ctgcataccg
    32461 aacagcatgg ctacagtgag aactatgttc cgtacctggt taaccaggac acgctgtacg
    32521 gtacgggtca gctgccgaaa tttgctggcg atctgtttca tactcgtccg ctggaagaag
    32581 aagcagacac cagtaactat gcgctgatcc caacggcaga agttccgctg actaacctgg
    32641 tgcgcggtga aatcatcgat gaagatgatc tgccaattaa gatgaccgcc cacaccccat
    32701 gcttccgttc tgaagccggt tcatatggtc gtgacacccg tggtctgatc cgtatgcacc
    32761 agttcgacaa agttgaaatg gtgcagatcg tgcgcccgga agactcaatg gcggcgctgg
    32821 aagagatgac tggtcatgca gaaaaagtcc tgcagttgtt gggcctgccg taccgtaaaa
    32881 tcatcctttg cactggcgac atgggctttg gcgcttgcaa aacttacgac ctggaagtat
    32941 ggatcccggc acagaacacc taccgtgaga tctcttcctg ctccaacgtt tgggatttcc
    33001 aggcgcgtcg tatgcaggca cgctgccgca gcaagtcgga caagaaaacc cgtctggttc
    33061 ataccctgaa cggttctgga ctggctgttg gtcgtacgct ggttgcagta atggaaaact
    33121 atcagcaggc tgatggtcgt attgaagtac cagaagttct gcgtccgtat atgaacggac
    33181 tggaatatat tggctaatac ccaatttttc tgaatctaaa aagcgcctgc gggcggtttt
    33241 tgcatgagat taattaactg aaaataaaag ggatttattt tcaggtgtcc atataatgtc
    33301 catctttgaa acaaaaaagc ccgcagtttt tacgctacgg gctttgtttt tctttgtgac
    33361 ttgcgtgtga gggtgctact ttcgaccgaa cctttggcaa ccggatagcg ggggctttca
    33421 cccccgctgc ggtttcttac ggtttacact gcaagaacgc cgcaaattct gaccacatga
    33481 tgttaagtct gaactcacac ggcgaacggt gtatcatcca gactgtcaac aacatggtta
    33541 agcataaaat gctcacgatg atcgtttttt gcggcatagc gcttgctcct ttttcaaaga
    33601 ggcgctaacc ttccacttgc gaaggttaga tggttagggc ctcgggttaa acaaatgttt
    33661 gactcggggc cttttcacat ccggccttca ggtaatcctt ccagccgtca gccgaaaggc
    33721 acccacgtgt aatctactga tttccctttg tgcgcgcaat gctcagtgtt gaaactgttt
    33781 agggggaggg cggcaaaaaa gcccgcatat tgcgggcaaa tacgtctgta atccaatgga
    33841 gaaaggggtg ccatatcgac tgaacccgac aaccggatag cgggggcttt cacccccgct
    33901 gcggtttctt actgtttaca ctgcaagaac gccgcaaatt ctgaccacat gatgttaagt
    33961 ctgaactcac acggcgaacg gtgtatcatc cagatggcca acaacatggt taagcataaa
    34021 atgctcacga tgatcgtttt ttgcggcata gcgcttgact ccttttacag ggaggcgcta
    34081 accttccact tgtctagggt aggaatggtt agggcctcgg gttaaacaat atgtttgact
    34141 cggggccttt ccacatccgg ccttcaggtt ttccttccag ccgtcagccg aaaggcaccc
    34201 gcgcgtaatg tactggtttt aaagccatac cgcaatcctc cagttgtagc gttaactgac
    34261 tgcgtccctg atgggatttg cgcattgtct cccgtggaat atctttatcg tggaagatca
    34321 actgaggctg gccaataatt ctgtgcacac tttccattgt tgaaaatgtg gcgctgcact
    34381 caaggttatt gcactgatag tatttgtagc ggatggtgcc ggtatcattg gcggctttac
    34441 tggtacggat ccgggcagat gcgccgcaga atggacaatg aaacattgat atacccccct
    34501 ggctgaactg gggttgaact cgtcgccatt ctaaccattg ctttcctttt cggctatcca
    34561 ctcgggtatt cttgcctcca gttcaagcct tgtggtgaag ccgcttccgt cgatattgtg
    34621 ttccactttt gcaatgagcc agtcctgatt gtctatttcg gttttaaagc cggttaccgt
    34681 gccatgcatt tcagggtaaa gctccgctct gccccgggcc agcgtgatgg agaattcagc
    34741 cgcaccgcgc tgtaatt
//
LOCUS       DAZGVQ010000035        33739 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000035 DAZGVQ010000000
VERSION     DAZGVQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33739
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.035"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            156..1214
                     /gene="citC"
                     /locus_tag="ACHLAG_001673"
     CDS             156..1214
                     /gene="citC"
                     /locus_tag="ACHLAG_001673"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFP0654922.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1229..1525
                     /gene="citD"
                     /locus_tag="ACHLAG_001674"
     CDS             1229..1525
                     /gene="citD"
                     /locus_tag="ACHLAG_001674"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFP0654923.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1522..2430
                     /gene="citE"
                     /locus_tag="ACHLAG_001675"
     CDS             1522..2430
                     /gene="citE"
                     /locus_tag="ACHLAG_001675"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFP0654924.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2441..3973
                     /gene="citF"
                     /locus_tag="ACHLAG_001676"
     CDS             2441..3973
                     /gene="citF"
                     /locus_tag="ACHLAG_001676"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFP0654925.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3977..4528
                     /gene="citX"
                     /locus_tag="ACHLAG_001677"
     CDS             3977..4528
                     /gene="citX"
                     /locus_tag="ACHLAG_001677"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFP0654926.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4503..5381
                     /gene="citG"
                     /locus_tag="ACHLAG_001678"
     CDS             4503..5381
                     /gene="citG"
                     /locus_tag="ACHLAG_001678"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFP0654927.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5432..6895
                     /gene="citT"
                     /locus_tag="ACHLAG_001679"
     CDS             5432..6895
                     /gene="citT"
                     /locus_tag="ACHLAG_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFP0654928.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7009..7815
                     /gene="rna"
                     /locus_tag="ACHLAG_001680"
     CDS             7009..7815
                     /gene="rna"
                     /locus_tag="ACHLAG_001680"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFP0654929.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8045..8455
                     /gene="rnk"
                     /locus_tag="ACHLAG_001681"
     CDS             8045..8455
                     /gene="rnk"
                     /locus_tag="ACHLAG_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFP0654930.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8593..8682
                     /gene="yldA"
                     /locus_tag="ACHLAG_001682"
     CDS             8593..8682
                     /gene="yldA"
                     /locus_tag="ACHLAG_001682"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFP0654931.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYFENHS"
     gene            complement(8686..9924)
                     /gene="ybdR"
                     /locus_tag="ACHLAG_001683"
     CDS             complement(8686..9924)
                     /gene="ybdR"
                     /locus_tag="ACHLAG_001683"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFP0654932.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10145..10573
                     /gene="uspG"
                     /locus_tag="ACHLAG_001684"
     CDS             10145..10573
                     /gene="uspG"
                     /locus_tag="ACHLAG_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFP0654933.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10694..12259)
                     /gene="ahpF"
                     /locus_tag="ACHLAG_001685"
     CDS             complement(10694..12259)
                     /gene="ahpF"
                     /locus_tag="ACHLAG_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFP0654934.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12388..12951)
                     /gene="ahpC"
                     /locus_tag="ACHLAG_001686"
     CDS             complement(12388..12951)
                     /gene="ahpC"
                     /locus_tag="ACHLAG_001686"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFP0654935.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13323..14069
                     /gene="dsbG"
                     /locus_tag="ACHLAG_001687"
     CDS             13323..14069
                     /gene="dsbG"
                     /locus_tag="ACHLAG_001687"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFP0654936.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14278..15180
                     /gene="ybdO"
                     /locus_tag="ACHLAG_001688"
     CDS             14278..15180
                     /gene="ybdO"
                     /locus_tag="ACHLAG_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0654937.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15327..16547
                     /gene="ybdN"
                     /locus_tag="ACHLAG_001689"
     CDS             15327..16547
                     /gene="ybdN"
                     /locus_tag="ACHLAG_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP0654938.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARRMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            16520..17148
                     /gene="ybdM"
                     /locus_tag="ACHLAG_001690"
                     /pseudo
     CDS             16520..17148
                     /gene="ybdM"
                     /locus_tag="ACHLAG_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
     gene            complement(17149..18309)
                     /gene="ybdL"
                     /locus_tag="ACHLAG_001691"
     CDS             complement(17149..18309)
                     /gene="ybdL"
                     /locus_tag="ACHLAG_001691"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFP0654939.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKGDILVNALNESRLEILPCEGTYFLLVDYSEVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            18418..19506
                     /gene="hcxA"
                     /locus_tag="ACHLAG_001692"
     CDS             18418..19506
                     /gene="hcxA"
                     /locus_tag="ACHLAG_001692"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFP0654940.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(19516..19713)
                     /gene="ybdD"
                     /locus_tag="ACHLAG_001693"
     CDS             complement(19516..19713)
                     /gene="ybdD"
                     /locus_tag="ACHLAG_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP0654941.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(19889..21994)
                     /gene="cstA"
                     /locus_tag="ACHLAG_001694"
     CDS             complement(19889..21994)
                     /gene="cstA"
                     /locus_tag="ACHLAG_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFP0654942.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(22175..22588)
                     /gene="entH"
                     /locus_tag="ACHLAG_001695"
     CDS             complement(22175..22588)
                     /gene="entH"
                     /locus_tag="ACHLAG_001695"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFP0654943.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(22591..23337)
                     /gene="entA"
                     /locus_tag="ACHLAG_001696"
     CDS             complement(22591..23337)
                     /gene="entA"
                     /locus_tag="ACHLAG_001696"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFP0654944.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLVLSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(23337..24194)
                     /gene="entB"
                     /locus_tag="ACHLAG_001697"
     CDS             complement(23337..24194)
                     /gene="entB"
                     /locus_tag="ACHLAG_001697"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFP0654945.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(24208..25818)
                     /gene="entE"
                     /locus_tag="ACHLAG_001698"
     CDS             complement(24208..25818)
                     /gene="entE"
                     /locus_tag="ACHLAG_001698"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFP0654946.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVSELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCLLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(25828..27003)
                     /gene="entC"
                     /locus_tag="ACHLAG_001699"
     CDS             complement(25828..27003)
                     /gene="entC"
                     /locus_tag="ACHLAG_001699"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFP0654947.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            27378..28334
                     /gene="fepB"
                     /locus_tag="ACHLAG_001700"
     CDS             27378..28334
                     /gene="fepB"
                     /locus_tag="ACHLAG_001700"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP0654948.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAAQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(28338..29588)
                     /gene="entS"
                     /locus_tag="ACHLAG_001701"
     CDS             complement(28338..29588)
                     /gene="entS"
                     /locus_tag="ACHLAG_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFP0654949.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            29699..30703
                     /gene="fepD"
                     /locus_tag="ACHLAG_001702"
     CDS             29699..30703
                     /gene="fepD"
                     /locus_tag="ACHLAG_001702"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFP0654950.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            30700..31692
                     /gene="fepG"
                     /locus_tag="ACHLAG_001703"
     CDS             30700..31692
                     /gene="fepG"
                     /locus_tag="ACHLAG_001703"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFP0654951.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRQMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHISRRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            31689..32504
                     /gene="fepC"
                     /locus_tag="ACHLAG_001704"
     CDS             31689..32504
                     /gene="fepC"
                     /locus_tag="ACHLAG_001704"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP0654952.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(32501..33634)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHLAG_001705"
     CDS             complement(32501..33634)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHLAG_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFP0654953.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         8064 a         9206 c         8521 g         7948 t
ORIGIN      
        1 gaaatactcc ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt
       61 tctcccaagt gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat
      121 cgataacaac atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc
      181 gcgtaaaacg ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg
      241 atttgagcgt tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta
      301 tcgcgtgcgg tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc
      361 gcggtgaagg actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc
      421 acagcacgca tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg
      481 gtttttccac gctgaccagc gtacccggcg tgatggtgct gatggaaaac agcgccacgc
      541 ggctgaaacg ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct
      601 gcattgtgat gaacgccaat cccttcacga atggtcaccg ttatctgatt caacaagctg
      661 cggcacagtg cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct
      721 atgaagaccg gttggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc
      781 atcgcggctc cgaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac
      841 agagcgtcat taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg
      901 ctcccgcgct gggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg
      961 cccagtacaa ccaggatatg cgctactggc tggaaacgcc gactatctcc gcaccgccca
     1021 tcgaactggt tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg
     1081 tacgtcaact gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca
     1141 cgctgcatta tttgcagaac ctgcttgagc actctcgcca ggacgcggca gctcgtcaaa
     1201 agacccccgc atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac
     1261 ccttgagtct ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct
     1321 gcaaatcaat agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga
     1381 cgttctcgcc cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact
     1441 ggactgcatt ttacgtgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc
     1501 tctgccatgg gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc
     1561 cgccgcagca tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc
     1621 tacccggctg atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac
     1681 accgcccgcc gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc
     1741 attgtgcgtg tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt
     1801 cgcggtggtg cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat
     1861 attgaaaaag agatcctgcg tatcgaaaaa gcctgtggtc gtgaacccgg cagcaccggc
     1921 ctgctggcgg cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct
     1981 tccgagcgtt tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca
     2041 gaacgctccc cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg
     2101 cgctctgcgg gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga
     2161 ttcctgcaag aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac
     2221 ccgcgtcaga ttgatctgtt gcacaacctc tacgcaccaa cccagaaaga agtggatcac
     2281 gcccgccgcg tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc
     2341 ctgaacggca agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctctcgt
     2401 gcagaacttt ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca
     2461 atctcaacga caagaacggg tagcggcctg gaatcgtcgc gctgaatgcg atcttgccgc
     2521 tttccagaac tcgccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg
     2581 cgccaacctg gaagaagcga ttcgtcgctc tggtttacag gacggcatga cggtttcctt
     2641 ccatcacgct ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa
     2701 gatgggcttt aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgtt
     2761 ggtagaacac attcgccagg gcgtggttac ccgcatttat acctccggcc tgcgcggtcc
     2821 actggcggaa gaaatctccc gcggtctgct ggcggaaccg gtgcagatcc actctcacgg
     2881 cggtcgtgtg catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt
     2941 cccgtcctgt gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc
     3001 cctcggctat gcaatggttg atgccgacaa cgcaaaacag gttgtgatgc ttaccgaaga
     3061 actgctgcct tatccgcata atccggcaag cattgagcaa gatcaggttg atttaatcgt
     3121 caaagttgat cgcgttggcg atgctgcaaa aatcggcgct ggtgctaccc gtatgaccac
     3181 taacccgcgc gaactgctta ttgcccgtag cgctgcggat gtgattgtca actcaggcta
     3241 cttcaaagaa ggtttctcca tgcaaaccgg caccggcggc gcatcgctgg cggtaacccg
     3301 tttcctggaa gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggtggtat
     3361 taccgcgacg atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtaca
     3421 gagctttgac agccatgctg cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag
     3481 cgccaaccag tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt
     3541 ggtactgagc gcgctggaaa ttgacaccca gttcaacgtt aacgtgctga ccggctctga
     3601 cggcgtactg cgtggtgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc
     3661 catcatcgtc gcgccgctgg tacgcggtcg tattccgact ctggtggata acgtgctgac
     3721 ctgcatcacc ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa
     3781 cccggcacgt ccggaactgg cagaacgtct gcaggaagcg ggcattaaag tggtttccat
     3841 tgagtggctg cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac
     3901 cgaccgcgtc gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca
     3961 ggtgaaggaa taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat
     4021 tcccgagctg ctcgtcagcc gggatgaaag gcaagcacgg caacacgtct ggctcaagcg
     4081 ccatcctgtt ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga
     4141 ggtcacacgc cgaattttta atcatggcgt gacagccttg cgtgccttag ccgcaaaaca
     4201 gggctggcaa attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt
     4261 gagcattgcc gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca
     4321 tcctctcggg cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg
     4381 ccgcgactat tcactgccgc ctcgccgctg cctgttgtgc gagcaaagcg cagccgtctg
     4441 cgcgcgtgga aaaacccatc aactgaccga tttactcaac cgcatggagg cactgctgaa
     4501 cgatgtcgat gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca ttaatcgatg
     4561 agtacgccct gctgggctgg cgcgccatgc tgactgaagt caatctgtca ccgaaaccag
     4621 gcctcgtgga tcgcattaac tgcggtgcgc acaaagatat ggcgctggaa gatttccacc
     4681 gcagcgcgct ggcgattcag ggctggttac cccgtttcat tgaatttggt gcctgtagtg
     4741 cagaaatggc accggaagcg gtactccacg gattacgccc aattggtatg gcttgcgaag
     4801 gtgatatgtt ccgcgccact gcgggcgtaa acacgcataa aggcagcatt ttttctttag
     4861 ggctgctatg tgcggcaatt ggtcgtttgc ttcaactcaa ccaaccggta acgccaacaa
     4921 ccgtttgttc tacggcggca agtttctgcc gtggcctgac cgatcgagaa ctgcgtacca
     4981 ataattcaca actgacggca ggtcaacggt tgtaccaaca gcttggcctt accggcgcac
     5041 gcggtgaagc cgaagcgggt tatccactgg tgatcaatca cgccttgccg cattacctca
     5101 ctctgctgga tcaggggtta gatcctgaac tggcattgct cgataccttg ctcctactga
     5161 tggcgatcaa cggcgatacc aacgttgcat cgcgcggtgg cgaggggggc ctgcgctggc
     5221 tacagcgcga ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc gccgatctcg
     5281 attatctccg gcagttcgac agggagtgta tcgaacgaaa tctcagtcca ggcggcagtg
     5341 ccgacctgct gatccttacc tggtttttag cacagattta attatttaag cacttgataa
     5401 atttggaaat attaattttc ggagaacccg tatgtcttta gcaaaagata atatatggaa
     5461 actattggcc ccactggtgg tgatgggtgt catgtttctt atccctgtcc ccgacggtat
     5521 gccaccacag gcatggcatt acttcgctgt gtttgtggca atgattgtcg gcatgatcct
     5581 cgagccaatt ccggcaacag cgatcagttt tattgcggtt actatttgcg ttattggcag
     5641 taattacctg ctctttgatg ccaaagaatt agctgaccca gcgtttaatg cgcaaaaaca
     5701 ggcgctgaaa tggggtctgg ctggtttttc cagcaccacg gtatggctgg tatttggcgc
     5761 atttattttt gcattagggt atgaagtttc cgggttaggt cgtcgcattg cccttttcct
     5821 ggtgaaattc atgggcaaac gcacgctgac gttgggttat gcgattgtca ttatcgacat
     5881 tctgctggca ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg tttttccggt
     5941 cattaaaaac ctgccgccgc tgtttaaatc attccccaac gatccgtccg cgcgtcgtat
     6001 tggcggctat ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt ccatgtttgt
     6061 caccggtgcg gcaccaaacg tgctgggtct ggagttcgtc agcaaaattg ccggtatcca
     6121 gattagctgg ttgcagtggt tcctctgctt cctgccggtt ggggttatct tgcttatcat
     6181 tgcgccgtgg ctttcctacg tgctgtacaa accggaaatc acacacagtg aagaagtggc
     6241 aacctgggcg ggtgatgaac taaaaaccat gggtgcgctg acacgcagag agtggacgct
     6301 gattggcctt gtattgctca gcttaggttt gtgggtattt ggcagtgaag tcattaatgc
     6361 tactgcggtt ggtctgctgg cagtttcgct aatgctggct ctgcacgttg tgccgtggaa
     6421 agacattacc cgctataaca gcgcatggaa cacgctggtc aacctggcaa ctctggttgt
     6481 gatggctaac ggcctgactc gttctggttt tattgactgg ttcgccggta ccatgagtac
     6541 gcacctggaa ggattctcac caaacgcaac ggtgattgta ctggttctgg tgttctactt
     6601 tgcacactac ctgtttgcca gcctgtctgc gcacaccgca accatgctgc cggttattct
     6661 ggccgtcggt aaaggtattc cgggcgtacc aatggaacaa ctgtgtatcc tgctggtgct
     6721 gtctatcggt atcatgggct gtctgacgcc gtatgcaacc ggtcctgggg tgattattta
     6781 cggctgtggc tatgtgaaat caaaagatta ctggcgtctt ggcgcaatct tcggggtgat
     6841 ttacatctct atgttgctgt tggttggctg gccgattctc gccatgtgga actaatacgt
     6901 tcgccaaacg ctgaataaat catcacgccc gccaggtaag cccacctggc gggctttttt
     6961 atgatttaat agatagtatt cagtgtatgt atgagttcca cacccattat gaaagcattc
     7021 tggcgtaacg ccgcgttgct cgcggtttct ctgcttccct tctcttctgc caacgcctta
     7081 gcgttgcagg caaaacagta tggcgatttt gatcgctatg tcctggccct ctcctggcaa
     7141 accggatttt gccagagtca acacgatcga aatcgtaacg aacgagatga atgtcgcctg
     7201 caaaccgaaa cgaccaacaa agctgatttt ctgaccgtac atggtctgtg gccaggattg
     7261 cctaaatcgg ttgctgcccg tggtgttgat gaacgccgct ggatgcgctt cggttgcgct
     7321 actcgcccaa tcccgaatct accagaagcg cgcgccagcc gaatgtgttc atcgccggaa
     7381 accggattat cactggaaac ggccgctaaa ctaagtgaag tcatgccagg agctggcgga
     7441 cgttcctgcc tggaacgcta cgaatatgcc aaacacggtg cctgctttgg ttttgatccg
     7501 gacgcatact tcggtacgat ggtacgcctg aatcaagaaa ttaaagagag cgaagccgga
     7561 aaattccttg cggataatta cggtaaaaca gtgagccgcc gtgactttga cgccgccttt
     7621 gccaaaagct ggggaaaaga gaacgtgaaa gcagttaagc taacgtgcca gggtaaccct
     7681 gcgtatttga ctgaaattca gatctcgatc aaagctgacg ccatcaacgc tccgctttct
     7741 gcaaactcat tcttgccaca accacaccca ggcaactgtg gcaaaacctt tgtgattgat
     7801 aaagtgggtt attaatcatc tgatttacaa aacacgctaa ttcgtcttcc ataaaccgtg
     7861 acgattaaga attatacata atgcgctgca ttgaggaatt ccctgaatgt gacgcaaatc
     7921 acttcaaaga gtgagcaaca gtcagtatca tcatgtgagt taaaccctcg ccgcctgacg
     7981 gtgagggttt tcttttgggt atgtttatct gcgacactcg cagtaccgac gacatggagt
     8041 aaaaatgtcc agaccaacta tcatcattaa cgacctggat gccgaacgca tcgatattct
     8101 gctggagcaa cccgcctatg ctggtttgcc aatcgccgac gcgttaaacg cagagttgga
     8161 tcgcgcccaa atgtgttcgc cagaagagat gccacacgac gtggtgacaa tgaacagccg
     8221 ggttaaattc cgcaatctta gcgatggcga agtgcgtgtg cgcacgctgg tgtatccggc
     8281 aaaaatgacc gatagcaata ctcagctttc cgttatggct ccggtaggtg ccgcactgct
     8341 ggggctgcgc gttggcgatt ccattcactg ggaacttccg ggcggcgttg caacccacct
     8401 tgaagtgctg gaactcgaat accagccaga agctgctggc gactacctgc tttaatccca
     8461 tctgcacaag cgataactcg ctttgcccag aggatgcact cgcatcctct ttacgcaaaa
     8521 tcagcaattt ttcctggttc tcactgttca cttttgctgc aaaattattc cgtttcgttt
     8581 gtgggaataa ccatggcaga agcattttac atcctgatag gatttctgat tatggcggcg
     8641 attatcgtca tggctgtcct ttacttcgaa aaccactcct gacgatcata ttgttccccc
     8701 cggcatcgca ttcaccagac ctgaaaccgc cttctgcgcc gcctctgcgc tttgtgcacc
     8761 gggtaccaga atcaccttac ggcactcctc ttcacgtttt tcgaaaatct catatccccg
     8821 ggcggcctct tcaaacggca tatagtgggt aacaatttct tctggtttca gtaatccttt
     8881 ctcaattaac ggtaataatt ctcccagcca tgcgtgaacg tgggtctgtc ccattttaaa
     8941 cgtcaaccct ttatcaaagg cgtcgccaaa caggaaaccg tgaataaatc cagcgtagac
     9001 gcccggtacg ctaacaatgc cgccacgcct gaccgccgca atacactggc gcaacgcttt
     9061 accgctgctg ccctccagtt tcaggttagt cagcaccgtt tccgtggtgc tgcctttcgc
     9121 ttcaaaaccg acggcgtcta ttactgcatc cacgccccgg tgacctgccg tttgttcaat
     9181 aattgactgt gccggatcgc tatcttcatc gaaattaatc gggatcgcgc cgtagcggtc
     9241 agcggcgaaa cgcaagcggt agggatgatg atcaacaaca aaaatctgtt ccgcaccgag
     9301 caaccgtgca caggcgattg tcaacaatcc cactggaccg gcaccataga ctgcaacgct
     9361 tgaaccttgt tggatctgcg cattttttgc tgcctgccat gccgttggca gaatatcaga
     9421 aaggaaaagg gctttatcat ctgaaagcaa aggcggtact ttaaacggcc ccacattccc
     9481 tttagggacg cggacatatt ccgcctgccc accaggaacg ccgccataca ggtgactata
     9541 accaaacaat gccgcgggag ctggtatctg ttttttattg agcgcagcgc ctttacccgc
     9601 attggtattt tcgcaggcgg catattgctg taatcgacag aaaaaacagt cgccacaagc
     9661 aatgacaaac ggaattacca cccggtcgcc tttttgcaaa ttttttacgt cctttccggt
     9721 ttcaacgact tcccccataa attcatgacc aaaaatatcg ccatgtttaa cctggggtat
     9781 tttgcctcga taaagatgga ggtcagagcc acagatcgcc gttgccgtaa tacgcagaat
     9841 aatatcatct gcctgttcaa tgcccggatc gggaacattt tctacctgaa cgtgatgtgg
     9901 gccgtgataa gtcaatgctt tcatgcaacc tccagagaca gttcaaaaaa gagtcagtct
     9961 ttaagggtag ctatcacagc gggaaaacgg gcggtaccgc aggttttggg agaattcctg
    10021 gtgttcagtg agaatatatt aatcacagaa accgttcgta aggaaaaaga accagacaaa
    10081 tatcagggat aaatattata atcatggcga atgatttgtt catgattaac agggagaaag
    10141 ggttatgtat aagacaatca ttatgccagt tgatgtattt gaaatggaat tgagcgacaa
    10201 agctgttcgc cacgctgaat tcctcgccca ggatgacgga gttattcatc tacttcacgt
    10261 actacccggg tcagccagcc tgagcctgca ccgttttgcc gctgatgtgc gtcgttttga
    10321 agagcatctg caacatgaag cagaagaacg tctgcaaacg atggtcagcc acttcaccat
    10381 cgatccttcc cgcattaaac aacatgtccg ttttggtagc gtgcgggatg aagtcaatga
    10441 gttggcagaa gaactggggg ctgatgttgt agttattggt tctcgcaacc catcgatttc
    10501 gacccatctg ttaggttcta acgcctcgag cgtgatccgc catgccaatc tgccggtgct
    10561 ggttgtgcgt taattgccag aataaatatc ccgccctgcc cggagccatc ccggcagggc
    10621 tttttaatgc cataaaaaag ccgccaggtt tgaccccggc ggctaagcaa ttgcaggtga
    10681 atcttacttc ttcttatgca gttttggtgc gaatcaggta gtcaaaagca ctcagagagg
    10741 ctttggcacc ttcgccagtg gcgatgatga tctgcttgta cggaaccgtc gtacagtcac
    10801 ccgctgcgaa tacgcctttg acgttggttt cgcatttcgc atcaatgata atctcaccca
    10861 tgcggttacg ttcgactgcg ccttccagcc agttggtgtt cggcagcaga ccaatctgga
    10921 cgaaaatacc ggccagttcg atgttgtgaa tatcgccgct gacacgatct cgatattcca
    10981 gaccaacgac tttgctgccg tcgcctttca cttccgtggt ttgcgcattc agaataatgt
    11041 cgacgttttt caggctgcgc agtttgtcct gcagcacctg gtcggctttc atttctggcg
    11101 caaattccag cagagttacg tgctcaacga tacccgccag gtcgattgcc gcttccacgc
    11161 cggagttacc tccgccgata accgctacgc gtttgccttt aaacagcggg ccgtcgcagt
    11221 gcgggcagta ggtcacgcct ttggtgcgat actgatcttc gcccggaacg ttcatgttgc
    11281 gccattttgc accggtcgcc acgataatgc tgcgtgcttt cagtaccgcg ccagaagctg
    11341 tttcaatctg gtgtagacca ccttcaaccg ctgccgggat cagtttgctg gcgctctggc
    11401 tgtcgatcac atcaacgtcg tattcatcaa cgtgaacttt cagtgcgcct gccagcttct
    11461 gcccttcagt cttcggtaca gaaatgtagt tttcgatatc aacggtatcg aggatctgac
    11521 caccaaaacg ttcgcccatc agaccggtac ggatgccttt acgtgcggag taaattgctg
    11581 ccgctgcacc cgccgggccg gaaccgacga ttaatacgtc ataagcatca cgcttgttca
    11641 gctcttctgc cgcacgtttt tccgcgccag tatcaatttt ggcaacgatt tcagtcaacg
    11701 tcatgcggcc ctgaccaaac tctttcccgt ttacgaacac tgccggaacg cccatcacgt
    11761 tgcgatcggt gatttcgttc tggaaggtgc cgccgtcaat tgcagtgtgc ttgatgcgcg
    11821 ggttcagtac gctcatcagg ttcagcgcct gcaccacgtc cgggcagttg tggcaagaga
    11881 gcgagtaata ggtttcgaat tcaaaatcac cgtcaatatg gcgaatctgc tccagcagag
    11941 actgcgcttc tttcgacgga tgaccaccgg tccacagcaa cgccagtacc agcgaggtga
    12001 actcgtggcc cagcggggag cctgcaaaac gtggcccctg gttggaacct gggttggtga
    12061 tcaggaaaga cggcttacgc accggcaagc tgttatcttc tttaaaggtg actttgtctg
    12121 acagttctgc gatttcagcc aacagttcct tgatttctgc cgatttagcg ctgtcatcca
    12181 gcgtggcaat taactcaaca ggcttggtca atttctcaag gtaagccttg agttgagttt
    12241 tcatatttgt gtcgagcatg tttatctcct gggctttaaa catcatcatg caagcggcct
    12301 ctgcggctac ctggatgcag cttgcatcat ggtgcgggga agaaaatcgg gtgccacagc
    12361 acccgattaa ttacgcgatt gcggaactta gattttacca accaggtcca gagacggagc
    12421 cagagttgct tcaccttctt tccatttagc cgggcaaact tcacctgggt gagaagctac
    12481 gtactgtgct gctttgattt tacgcagcag gtcagacgcg tcacggccaa tgccttcagc
    12541 ggtaacttcg attgcctgga tgataccctg cgggtcaaca acgaaggtcg cacggtcagc
    12601 cagaccttca tcttcacgca tgttgtcgaa gttacgggtc agggcgccag tcgggtcgcc
    12661 gatcatcgca tatttgattt tagcgatggt ttcagagctg ctgtgccatg ctttgtgggt
    12721 gaagtgagta tcggtagata ctgcgtatac gtctacgccc agtttctgca gttcttcgta
    12781 gtggtcagca acgtcaccca gttcggtcgg gcatacgaaa gtaaagtcag ccgggtagaa
    12841 gaagaagacg ctccagcggc cttcggtatc tttttcggtg atttcgatga attcgccgtt
    12901 tttgaatgcc tggtttttaa aaggtttaat tttggtgtta atcaaggaca tctatacttc
    12961 ctccgtgttt tcgatgagat gtaaggtaac caatttttgc cgattcggct aatgcgtttc
    13021 cattatcaaa tcgataagtt ttaccttaca accttcgtaa gacaactttg tgaactttgc
    13081 accgccagcg gcgataaaaa gtaaaatggc cccctttcca ggtgaccgtg taatctgaac
    13141 taccctttgg tatcttcagc gcctgcctgc aggctgaggt gttgccctta caaatgcaac
    13201 aacgacatgg attacaacac cctcataaac aaagggcaat cacctgatct aagctcttac
    13261 ctatgacagt gataggttat gccttttact cgacttttgc actgactgaa aaggacaaat
    13321 taatgttaaa aaagatactt ttactggctc tgcttcctgc aatcgccttc gcagaggaac
    13381 ttccttcccc agtaaaagcg attgaaaaac agggcattac aatcatcaaa acgttcgatg
    13441 cccccggagg aatgaaaggt tatctcggaa agtatcagga tatgggcgtc accatctacc
    13501 tgactcccga tggtaagcac gctatctctg gttacatgta caacgagaaa ggtgaaaacc
    13561 tgagtaacac acttatcgaa aaagaaattt acgcaccagc cggacgcgaa atgtggcaac
    13621 ggatggaaca atcccactgg ctcctcgacg gtaaaaaaga tgcgccggtc attgtctacg
    13681 tgttcgccga tccgttctgc ccatattgta aacagttctg gcagcaggcg cgcccgtggg
    13741 tagattctgg caaagtgcaa ttaagaacat tgttggttgg ggttatcaag ccagaaagcc
    13801 cggcgacagc agcggcaatt cttgcctcca aagatcccgc aaaaacctgg caagaatatg
    13861 aatcctctgg tggcaagctt aagctaaacg tgcctgcaaa cgtaagtaca gaacaaatga
    13921 aagtgttaag cgccaacgag aaactcatgg acgatctggg ggcaaatgtc acaccagcga
    13981 tctattacat gagtaaggaa aatacgctgc aacaggccgt ggggttgccc gatcagaaaa
    14041 cgcttaatat cattatgggg aataaataaa agataattca tggatggttg aaatacatcc
    14101 ctgcttttca accatcctaa aaaagcatga attgctcgct ctgccctaag tggctatgct
    14161 attttagaaa aacttaatcc tgagttaagc aaaaaaatat aatcaataaa aatatatacg
    14221 caaaaaacat tgattaagtg aatataccat ggaagaaaaa tataaccgga gtagtgtatg
    14281 gccaatctct acgacttgaa aaagttcgac ttaaaccttc ttgtcatatt tgagtgtatt
    14341 taccagcatt taagcattag caaagctgct gaatcactat atatcacccc atcagccgtg
    14401 agccagtcgt tacaacgctt acgtgcgcaa tttaatgacc ctttattcat tcgctcagga
    14461 aaaggtatcg cgccgacgac aacagggttg aatctgcatc accatcttga gaaaaacctc
    14521 aaaggccttg aacaaacaat taatatagtc aataagtccg aactaaagaa aaactttatt
    14581 atctacggcc ctcagctcat ttcttgctct aataatagta tgctgatccg ctgcctacgg
    14641 caagatgcct ctgtcgagat tgagtgccat gatatcctca tgtcagcaga gaatgctgaa
    14701 gaactgttgg ttcaccgcaa agccgatctg gtgatcaccc aaatgccagt gatcagccgt
    14761 tccgtcattt gcatgccatt gcatacaatt cgcaatactc taatttgtag taataagcat
    14821 ccgcgaatta ctgacaatag cacttatgaa caaattatgg cagaagagtt cacccaatta
    14881 atatccaaat cagcaggagt tgatgatatt caaatggaaa tagacgagaa gtttatgaat
    14941 cgcaaaattt catttcgtgg ttcctctttg ctgacaatca ttaatagcat tgcagttaca
    15001 gatttactgg gaatagtccc ctatgaactt tataattcct accgtgactt tcttaattta
    15061 aaagagataa aactagaaca tccgttacct tcaataaagc tttacatttc ctataataaa
    15121 tcttctttaa ataatttggt gttctcccga tttattgacc gtctaaatga gagtttttga
    15181 tatataacta cagacagcta ctataacttt catctattta ttcacagcgc gaatgaaaaa
    15241 taaaacactc tgctttgcag tgtttacttt ataattaaca gacttatctc actgatcacc
    15301 ctgtaacgtt cagagagcgt cttcagatgt ctatttataa aattcctctt ccgctcaata
    15361 ttctggaagc tgcacgagag cgtattacct ggacactcaa caccctaccc cgcgtttgtg
    15421 tctcattttc tggcggcaaa gactctggtt taatgctgca tctgacagcc gaacttgccc
    15481 gacgaatggg caaaaaaatc tgcgttttgt ttatcgactg ggaggcgcaa ttctcttgca
    15541 ccattaacta tgttcagtcc ctgcgcgagt tgtacaccga tgtcatcgaa gagttttact
    15601 gggttgcgct cccgcttacg acgcaaaatt ccctttcaca ataccaaccc gaatggcagt
    15661 gctgggaacc tgatgtcgaa tgggtgcgtc agcccccgca agatgcgata accgatcctg
    15721 actttttctg cttttaccag cctggcatga ccttcgaaca atttgtacgt gagtttgccg
    15781 aatggttttc acaaaaacgt ccggcggcga tgatgatcgg catccgtgcg gatgagtcct
    15841 acaaccgttt tgtcgccatc gccagtttaa ataaacaacg ttttgccgac gataaaccat
    15901 ggactacggc cgcaccaggc ggtcatagct ggtacattta ccccatttac gactggaaag
    15961 tggctgatat ctggacctgg tatgcaaatc atcaaagtct ctgcaaccca ctgtataacc
    16021 taatgtatca ggcaggcgtt cctctgcgtc atatgcgaat ttgcgaacct tttggcccgg
    16081 agcaacgaca aggattatgg ctctatcacg ttatcgaacc ggatcgctgg gcagctatgt
    16141 gcgcacgagt cagcggggta aaaagtggcg gcatttacgc cggacatgac aatcatttct
    16201 atgggcatcg gaaaatcctc aagccagaac atttagactg gcaagaatat gcattattgc
    16261 tgctcaatag catgccggaa aaaacagctg agcattaccg caataaaatc gccatttatt
    16321 tgcactggta tcagaaaaaa ggcatcgagg ttccacaaac ccagcaaggg gacattggcg
    16381 cgaaagatat cccctcctgg cggcggatat gcaaagtttt actcaataac gattactggt
    16441 gtcgagcatt atcatttagt cctacgaaag cgaagaacta tcagcgttat aacgaacgga
    16501 taaaaggaaa acgtcaggaa tgggggatac tatgcaacaa cgattaacgc aggatttaac
    16561 gcaatttctc gccagcttgc cggaagatga tcgcatcaaa gcgattaatg aaatccgcat
    16621 ggcgatccat caggttagcc cctttcgtga agagccagtg gattgcgttc tgtgggttaa
    16681 aaacagccaa cttatgccta atgattacaa cccaaacaac gtggcaccac ccgaaaaaaa
    16741 ctgctgaaaa aatccattga aatagatggt tttacccaac ccattgtggt cacgcatacg
    16801 gataaaaatg ccctggaaat cgtggatggt tttcaccgcc atgaaattgg taaaggctca
    16861 tcatcattaa aattacgctt aaaaggttat ttgccggtga cctgtctgga aggtacgcgt
    16921 aatcaacgca tcgccgcgac tattcgtcat aatcgcgctc gtggacgcca tcaaatcacc
    16981 gccatgtcgg aaatcgtccg tgaacttagc cagttgggat gggacgataa taaaattggc
    17041 aaagagctag gaatggacag cgacgaagtg ctgcgcctga agcaaattaa cggcctgcaa
    17101 gagctgtttg ccgaccgtca atattcccgc gcctggacgg ttaaataact aaagctggcg
    17161 caggcgttca gctgctgcca gcaacgtcga ttccttcttg gcaaaacaga gacgaatcag
    17221 tttatgtggg aagggatcgg cgcaaaacac cgacagcgga atcgccgcta cgccgtgctc
    17281 ctgcgtcagc cactggcaaa actcaacatc atccagggta gaaacctcgc tgtaatccac
    17341 cagcaaaaag tatgtacctt cacacggtaa aatctccagc cggctttcat ttaaagcatt
    17401 caccagaata tcgcccttct ggcgataaaa gtccggtaac gcaagataat gctcaggttc
    17461 tgcacgtagc atatcagcaa gcgccagctg tgccggggta ttcaccgaaa aggtcagata
    17521 ctgatgtacc ttgcgaattt cggcgctgat gggcgctggc gcaacacaat aacccacttt
    17581 ccagccggtc atatgatagg tcttgccaaa tgaagaaacc gccactgccc gctcacgcag
    17641 ctgcggatgc gccagcacac tggcatggcc ctgttgtgaa aagttgatgt gctcgtagac
    17701 ttcatcgcta atgacaaaaa tctcgtgccc ggcgatcgcc tgccacaaag cggcgaaatc
    17761 agcctgctgc cagacagttg cactggggtt atgcggagtg ttgaggatca ccagtctggt
    17821 gcgctcgctt aataatgcgg caaattcctg ccagtcaacg cgaaaatgcg gtggttgcag
    17881 tgccatacgc ttcactattc ccccagaaag cgcgatggcg ggggagtaac tgtcatagct
    17941 gggatcaaaa caaatcactt catcgccatt gcgcaccagt gcggtaatcg ccgcgtataa
    18001 cgcttccgtc gcccctgccg ttacggtgat atcgctatcg gcatctggtt gatagccata
    18061 caaacgttcc gttttctgag caatcgcctc gcgcaaggcc tgcacgccgg tcatgggcgc
    18121 gtattggttt gccccctggg caacgtggta cgccagccgc tcctgtaaat agcgcggacc
    18181 atcaaaatca ggaaagcctt gcgacaggtt aatcgcctgg tgttgctgcg ccagcgcgct
    18241 catctgggtg aaaatagtgg tgccaagttg tggaagtttg ctttgtggaa tcagagggtt
    18301 atttgtcatt tttattgtac ctgacgaagt tgcgtgttga aggcactata acacgatgct
    18361 agtatttggc aatcaagacg tttagatgtc taaataaaac aataaggaca acacaacatg
    18421 cctcacaatc ctatccgcgt ggtcgtcggc ccggctaact acttttcaca tccaggaagt
    18481 ttcaatcacc tgcacgattt tttcactgat gaacaacttt ctcgcgcggt gtggatctac
    18541 ggcaaacgcg ccattgctgc ggcgcaaacc aaacttccgc cagcgtttgg acttccaggg
    18601 gcaaagcata ttttgtttcg tggtcattgc agcgaaagcg atgtacagca actggcggct
    18661 gagtccggtg acgatcgcag cgtggtgatt ggcgtcggcg gcggcgcact gctcgacacc
    18721 gcgaaagccc tcgcccgccg tctcggtctg ccgtttgttg ccgttccgac gatcgccgcc
    18781 acctgcgccg cctggacacc gctctccgtc tggtataacg atgccggaca ggcgctgcat
    18841 tatgagattt tcgacgacgc caattttatg gtgctggtgg aaccggagat tatcctcaat
    18901 gcaccgcaac aatatctgct ggcggggatc ggtgacacgc tggcgaaatg gtatgaagcg
    18961 gtggtgctgg ctccgcaacc agaaacgttg ccgctaaccg tgcgactggg gatcaataat
    19021 gcgcaggcca ttcgcgacgt attgttaaac agtagcgaac aggcgctgag cgatcagcaa
    19081 aatcaacagt taacgcaatc attttgcgat gtggtggatg ctattattgc tggtggtggg
    19141 atggtcggtg gtctgggcga tcgttttacg cgtgtggcgg cggctcatgc cgtgcataac
    19201 ggtctgactg tcctgccgca aaccgagaaa tttctccacg gcaccaaagt cgcttatggc
    19261 attctggtac aaagcgcctt gctgggtcag gatgatgtgc tggcgcaatt aaccggggcg
    19321 tatcagcgtt ttcatctgcc gaccacgctg gcggagctgg aagtggatat caataatcat
    19381 gcggagatcg acaaagtgat tacccacacc ctgcgtccgg tggaatccat tcattacctg
    19441 ccggtcacgc tgacaccaga tacgttacgt gcagcgttcg aaaaagtgga atcgtttaaa
    19501 gcctgacgta catatttagc agcagcgcgc gccgcctttt ccaccgtagc gcgcgtcctg
    19561 ccgctcacgg aaaaactcct cgtaggtcat cggcgtttga tcgggatggt taacccgcat
    19621 atgttcgaca tagttgtcgt aatcaggcat accaatcatc agcttcgccg cctgacctaa
    19681 atattttccg gcttttgcca gtgaatcaaa catcttggcc tccgctaacc atgttgctga
    19741 aagagtgccg gatgcggcgt gaacgcctta tccggcctac aaatgctatc aacgcgctcg
    19801 gtcagtcccc tcgccccacc ggggagaggg tgagggggaa ccctcggcgc catgccaaca
    19861 ttcgccaaca ttccccctca ctctgacttt agtgtgcgcc ttttgcctgc gccacgatct
    19921 cctcgacatt ttccggcatt ggctcatacg gcgtttcttt cgccgttggc ttcggctctt
    19981 tcaatgccgc aagtgccgtc ttaatcgaga acagtgccag aaccaccacg accaccataa
    20041 agaagatggt taacccggca tccagacggt tgttgaacac cagttgcgcc agctgtgact
    20101 cagtatactg cgacggaata ttgccgctgt cgatcattgc ctggaacttg ttagcaatgg
    20161 ccaggaagcc gactttcgca tccgggctaa acgctttctg ccagcctgcg gtcagggtac
    20221 aaatcagtag ccaggccgtt ggtaccagcg ccacccaggc gtaacgttga cgtttcatct
    20281 tgaacaacac cacggcacag agcatcagcg ccatccctgc cagcatctgg ttggcaatac
    20341 caaacagcgg ccacagagtg ttaataccgc ccagcggatc gaccacgccc tgatggagga
    20401 agtagcccca cgccagtacg cacagcgccg ttgccagcag gttagcaggc agtgaatcgg
    20461 tccgcttcag gccaggagac accacgccca gcagatcctg caacataaag cgcgcagcac
    20521 gcgtacccgc atctaccgcc gtcagaataa acagagcttc aaacagaatg gcgaagtgat
    20581 accagaacgc cacatccatc ataccaccca gcgcaccgtg caggatgtaa gccatcccca
    20641 ccgccagcgt cggcgcaccg cccgcacggg aaatgatcga ctgttcaccc acttcgctgg
    20701 caatctggtt gagcgtatct ggtgtaatgc taaagcccca gctactcacc acctgcgcgg
    20761 cagaagcgac tacatccgcc gtccctgccg gagccagcac cgccattggg ctgttcatgg
    20821 caaaatacac gcccggatcg atgatacagg cagaaaccag cgccataatc gccacgaagg
    20881 attccatcaa catcccaccg tagccgataa agcacgcctg cccttcgttc gccagcatct
    20941 tcggcgtggt gccggaagag atcagcgcat ggaagccaga caccgcacca caggcgatgg
    21001 tgataaacag gaacgggaac aggttaccgg tccataccgg gccagtgcca tcgacaaatt
    21061 tggtcagcgc aggcatggtc agcgtcgggc gcataatcaa aatgcctacc gccagaccga
    21121 cgatagtccc gattttcagg aaggtcgaga gatagtcacg cggggccagc agcaaccaca
    21181 ccggcagcac cgccgccaca aaaccgtagc ccaccagcat ccaggtcagc tgcacgccgg
    21241 taaagtcaaa gtacggtgcc caggtcggac tttctgccac ccagccgcca gagataatgg
    21301 cgaaaatcag gaacaccagg ccaatcactg acacttcgcc aatgcggccc ggacgcagat
    21361 agcgcaggta gattcccata aaaatcgcca gcggaatggt gaacgcaacg gtatacgttc
    21421 cccacgggct atgagtcagg gctttcacca cgatcatcgc cagtactgca aggataatga
    21481 ccatgatcat aaagcaggct accagcgcaa tcaccccggc agttggcccc atctcttctt
    21541 tgaccagctc acccagcgat cgaccgtcac ggcgcgtaga aacaaacagc accatgaaat
    21601 cctgcaccgc accagcgaga accaccccgg cgagcagcca gatcatccct ggcaggtaac
    21661 ccatttgcgc cgccagtacc ggcccaacca acggacctgc tccggcaatg gccgcaaaat
    21721 ggtgaccgaa cagcactttc ttgtccgtcg gcacatagtc cagcccgtcg ttatggcgca
    21781 ccgctggcgt catacgcgtc gggtcaaccg ccagcacatt tttggcgata tacagcccat
    21841 aaaaacggta agcgatcaga tagatacaga ccgacgccac cacaatccac agcgcgttga
    21901 tctgttcccc acgatttaaa gcaatgtatc ccagagcaaa tgctcccatt acagagagca
    21961 ctgtccagac gaggtatttc cctgatttgt tcatagttgt tatccgtgtg cgtgtccata
    22021 gagatgttac attttgtttc tataacatct ttgtacgatt taacaacacg gtaaccatgt
    22081 aaatcggagt tgaaaccaat atttaactta acaatgttaa ctcgatcact ccgttaaccg
    22141 agttttcctg caatctcatt taattctgtc gggctcatcc caaaactgcc gtacccagcc
    22201 gacaagtgca gcaacgccgc ccctgttcat cgaaaacgac gatttcccag ctctgatttt
    22261 gccgcccaag atgcagcggc tggcagacgc cgcgtacctt gccttcagac accgggcgat
    22321 gatgggtggc gttaagttcc gtacccacca cacactgccc gtcacgggtc atcataaatc
    22381 cggccatcga tcccagcgtt tcagccagcg ccgccgacgc gccgccatgc agcaggccaa
    22441 acggctgatg agtacgggta tcaaccggca tttcggcttc cagcacatca tctcccagac
    22501 gggtatacac aattcccaga tgcgccacca ttgtgttatc gctggtggcg ttcagttcgt
    22561 cgagcgttaa atggcgtttc cagatcatgc ttatgccccc agcgttgagc cgccatcgac
    22621 cacaatatcc tgtagggtga tatggctggc gaggtcagag gcgaggaaca aaatcgtgtt
    22681 ggcgatctct tgtggacggg cgattttccc cagcggaatg ccgagtttaa actgctcgcc
    22741 aaagccgcga atacgctgtt cttcggcgtc atcgctcacc cacagcgtgc gttgcatatc
    22801 ggtgtcggtg gagccaggcg aaaccacatt acagcgcacg ccgctacccg ccagttccag
    22861 cccgacgctc aacaccaggc ttttcagcgc cgctttcgat gcgccataag cactcatacc
    22921 aatacgcggc gtgtgtgcgg cgtcggacgc cacagtgaca atcgccccgc cccgctgacg
    22981 gcgaaactgg ttcatggttt gctggaacag gttaaacgca ccgccgacgt taaccgcaaa
    23041 agtctgctgc cagtcctctt tactgagctg atcggtcgcg cccatgcgca aaattcccgc
    23101 cgcattgatc agcacgtcca gtcgctccgt ttcagctaac agtcgctgac atacctgcgc
    23161 cacctgcccg gcgtcggcaa catccattac ttcggtcgca aagggatatt gctcctgagt
    23221 gaacgcttga tcaaaacctg taactttcgc tcccgcctca acaaacgcca gcgccgtggc
    23281 gtagccgata cctttacctg cgccggttac ccagacattt ttaccgctga aatccattat
    23341 ttcacctcgc gggagagtag cttccaccag gcgtcgatgg tcgggttttt cgccagcatg
    23401 acaaagtcga tatcaccatg cactttgcgc cagcgcgccg ccagcgccat catgcgcacc
    23461 gaatccagac cgtagtcgat caggttgtcg tcatcgaacg gttcatcgga ctcgtccagc
    23521 aacggcagga taacctcacg cagcgccgct ttgctggcgg ggacaggtgc tggcagtaat
    23581 tcttcagtca tcaccacccg gccagaccgc ccggccacat atttcagcga catcaaatgc
    23641 tcgtcacggc tgaaatcggc cagcgcatcc gccaccataa acggtttaat atcgcgcata
    23701 aatgcgtcgg ttgcggtggt catacagcca atgtgggcat ataccccggt aataatcagc
    23761 tggttacgtc cactctcttt gagcatttgc tccagcggag aacgatgaaa cgcgctgtag
    23821 cgccacttca ccagcaccgt gtcgtcggca tctggcgtca ggcgatccac caccttttgc
    23881 tgttccggcg aacgggtcag gcccggcccc cacatatcat tcaacagcgc ccgatcttca
    23941 tcgctctgct ctttcggctg ggcggtgtaa taaaccggga tattgtgctg tttgcagtag
    24001 tcgcgcagcg cagcaatatt cgcgatcacc tgctccatca tcgggcagtt ctcgccccag
    24061 aagctgacaa aatagtcctg catatcatgg attaacaacg cggcacgttg cggttcaaag
    24121 gcccagtcga ctttattctt agggatatcg tgagactccg gcagtgcgta agcctgtaat
    24181 tttggaatag ccatcgtgtt ctctccttca ggctgatgcg cgtgacgcca gccactgacg
    24241 taattgtttt ttatcgactt tcccgaccgc cgtcagcgga agtgaatcta cacactccac
    24301 gcgatccggt aatttaaatt cggcaatacc ctgttcacgc aggaaacgac gcacctgcac
    24361 cgcgcgcagc ggttctttca ccaccagata agcgcagctt ttttcgccca tcagctcatc
    24421 ttccatgctc accagtgcgg cgtagatcac cgccgggtgg cgcagcagca ggttttcgat
    24481 ctcttcggca gcgatcttct caccgccacg gttgatctga tctttctcgc gcccctgcac
    24541 ggtgatgtac ccctctggat caatagagat cagatcgccg gaacagtaaa aaccgttggc
    24601 atcaaaggca ctggcattgt gctgcggact tttgtaataa ccgcggaagg tgtacggccc
    24661 gcgcgtcatc aggcgtccaa cttccccttg cggcagtgga tttccttcag catcagcaac
    24721 ccatacttca tcgtccggac acattgggta accctgggta tggataattt tctccgcgct
    24781 atcatcaagt cgggtgtagt tcaccagccc ttccgccatg ccaaacacct gctgcaacag
    24841 gcagccaatc tcagcgggaa tacgcgccgc aagggtggct gaaagacgtg cgccgccgac
    24901 ctgtaacagt ttcagcgagg caagctgcgc ccggctttca ccttcggtca gcgcctgcaa
    24961 ccacaggctg actgccggtg gcaccagcgc ggtgacgttc acctgatgtt tttcaatcaa
    25021 tgggaagcaa agcgtggcgc tgggatcggc cgccagaaca accgttcctc cggcaagaaa
    25081 gacgcccagc gatcccggcg aactcatggc gtagttatga gccgccggga tcgcgcacag
    25141 gtagcgtgtc tgttgtgtga actgacaaat ctcgacgcta cgacgcacgc tgtagtagta
    25201 gtcgttatga gtgcgcggga tcagtttcgg tgtgccggtg gtgccgccgg aaagctggaa
    25261 ataggccact tcatcagcag gtgatggcgt ggcagtaaaa tcctcagccg gatggttaat
    25321 cgcatcctgc aagttatgct caccgctgtc gttgtgcagt tgcaccacgc gaatggagga
    25381 atgttctgtg acgaaagtat tgaggaaatc atccccgcta aacagcgcat gttggcgatc
    25441 ggcaatcagc aatgcgggtt caatctggct ggcataggcg ttcagttcac tacgctgatg
    25501 gctgaacagc gccagcaccg gcgcaacgcc cagtttcagc agcgcgaaaa aggtaatata
    25561 caattcagag acgttaccca gttgtaccag cgcggtttca ccaggtttaa tgccctgacg
    25621 gcgtaaacta cacgcgaggt tatccgccgc ctgattcagc tcccggtaac tcaactgtcg
    25681 ctcgccgtcg ataaccgcga tgctgtcact cgcagcgtgg cgagtcagaa tgtcggtcag
    25741 cggcaaatcc tgccagtagc ctttttcccg atagcgacgg gcaaactctt ccggccagcg
    25801 ggtgaatgga atgctcatcc tcgctcctta atgcaatcca aaaacgttca acatggtaga
    25861 aagtttgacg cctgtttcgc gccactcacc caacggtgac gacgcaggca caatccccgc
    25921 tccggcaaac agacgcacct gattttcccg cagcttcgcg cagcggatgg tcaccaccca
    25981 ttcgccgtta ccttcgctgt cacaccaacc cacaatgccg ccaaacagtt cgcggtcgaa
    26041 tggctccagt tcagcaataa cctgggtcgc ggcctgatgc gggaaaccgc tcagcgcggg
    26101 ggttggatgc agcagacagg ccaaagtcag tgcgttttct tgcgaattcg ctttaccttc
    26161 aaagggagtt gcgagatgcc acagcgtcgg cgtggtaatc aattgtggag aggagggaac
    26221 gtgtaactca ctactgcgtt cgcgcagtac ctctttcatc gcctgagtca ccagttcatg
    26281 ttcatggcga tctttttctg acgccagcag acgattaccc gcttcgcgat cgagcacttc
    26341 atccggctga cgacgcgcgg aaccggctaa cggaatggag ctaaaacgct cgccgtcttt
    26401 acgtagcagc agttccgggc tggcccccag caggacgcca ccatcagcca gcgggacatg
    26461 gaagttgtaa ctaaccgggt tttgcgcaat caaccgttcc agcaatacgc cactatcaat
    26521 ggcggcgtca gtggtgatat caatcaatcg tgacaacacc actttgtcga cctgcggcgt
    26581 ggcggtaagt gcggcggcgc gagcaaccat ctgttcaaac gtggtttgtt caggaattgc
    26641 ctggcgttcc accacgttca gcgactggct gcgggtaaaa cggcgggctg aggtctgttt
    26701 ttcctggcgg gagaacgact gccaggattc gggaatatac agcgacgaag gctgacgtgg
    26761 atcgaaggga atcgccccga ccatcaccgg atttttgatg ccctgcgctt tggcatcggc
    26821 aaacagcgcg gcgagttttt gctggaaggg actgtcgggc gaatccccgt tcacagccgg
    26881 ttcatcgaag cgggcgaaac atcctgacgt cgtaaaactg cggtacggcg acataaagaa
    26941 aaagcgattg ggcgcaagtg ttgccatggt ctgctgtact tcctcagcca gtgacgtatc
    27001 catatcatcc tccacaaaat gataaaggct ttaataatga ttatcattta tattttcggt
    27061 cgctaaccta aaagcaaacg cgcattatgt caactcttga ggtaacgcaa tctgcaactc
    27121 ggggttgcat ctggttgtgg ttacaatgaa aatgagaagc attattgatg gattcgcata
    27181 agcgcaatgt gatgtcctgc gccgttctgc cccctctccc ttccagggtg agggctgggg
    27241 tgagggttaa tgttcgcacc agtgctggct gttcccctca ccctaaccct ctccccaaag
    27301 gggcgagggg acggattgtg cgctttgtcg aatttgtcat tacgccctta accttattaa
    27361 taacaggaag ctgatttgtg agactcgccc cgctctaccg caacgccctt ctattaacag
    27421 gacttttgct ttcaggaata gccgcagctc aggccgctga ctggccgcgt cagattactg
    27481 acagccgtgg cacacataca ctggaaagcc agccgcagcg tattgtttcc accagcgtca
    27541 ccctgaccgg ctcactgctg gcgattgatg ctccggtgat cgccagcggc gcgaccacgc
    27601 cgaataaccg cgtcgcggat gaccagggct ttttacgcca gtggagcaag gtggcgaaag
    27661 aacgcaaact gcaacggctc tatatcggcg aaccgagcgc cgaagccgtt gccgcgcaaa
    27721 tgccggatct gattttaatt agcgcaaccg gcggggattc ggcgctggca ctgtatgatc
    27781 agctttccac catcgccccg acattaatca tcaattacga cgacaaaagc tggcagtcgc
    27841 tgttaacgca gcttggcgaa attaccgggc atgagaaaca agcggcagag cggattgcac
    27901 agtttgataa gcaactggca gcggcgaaag agcaaatcaa attaccgccg cagccggtca
    27961 ctgccattgt ctataccgcc gctgcacaca gtgccaatct ctggacgccg gaatcagcac
    28021 aagggcagat gctggaacaa ctcggcttta cgctggcgaa gttgcccgca gggttaaacg
    28081 ccagccaaag ccagggcaaa cgccatgaca tcattcagct tggtggggaa aatctggctg
    28141 ctgggttaaa tggcgagtca ctattccttt tcgccggtga tcagaaagat gccgatgcga
    28201 tttatgccaa tccactgctc gcgcacctgc ccgcagtaca aaacaagcag gtttatgcgc
    28261 tgggaaccga gacgttccgc ctggattact acagcgccat gcaagtgctg gaacgactta
    28321 aggcgctgtt ttaagcatta actgtcagac gctgtcacct gcggcggcgt ctggcgaaaa
    28381 cgtcgcaact ccaccagcac cagcaataac aacacgccga taatcaacaa accaaaaccg
    28441 ctcgcgcttg cggaagcaac cggtgtcatc atcgcgccca aaccacccag cagcgccgcg
    28501 cctatggcat cgcccgtcac gttctgcgcc gtccacaaac cgttaatccg ccctaacatc
    28561 gcttccgggg tttgcgtttg cagcattgta tattgcagca acgaactaac cgcgctcagc
    28621 cagccgaaca gcgccagaca aaccacgcct aaaatccaca tcggcatcag gccaaacaga
    28681 ccaatggcga ggaacgatcc cagcgtggag agcagcatca ataaccctgg tcgcgcacta
    28741 tgtgccagct tcccgctggt taacgcacca atagccgcgc cgagcgggat cgccgcgtag
    28801 agaaaaccaa tctgtgccgc tgacatctgc cagttgtcag ccagcgccgg atacagtacc
    28861 cgcaccgcgc tcgccatcgt caataaacca cccagcagcg caatccctcc taccagcggg
    28921 ctggcgagca gaaaacgaaa tcctgccagt aatgatttca acggatgctc gcgcggctgc
    28981 ggtggcggtg gcaacgccgg aaggcttaac aacggtagca aggtaataaa cgtgcccgcc
    29041 gccgccagcc cgtagttcca ggctacgccg ccggtcgcca gcaataaacc gccaatcatg
    29101 ggcgaaatca ccgaccccag acgcacggtc aacatggtga tcgccccggc ctgcattaag
    29161 ttttcacgcc ctaccagtgc aggtgttgcc gccagtagcg ccgtaacacc aagtgatgcg
    29221 aaaaaaccat cccataaacc aagtaaatag attgccagca atgacggctc cggcagcagc
    29281 gcgttcaggc acagtccaat gaagccaatg ccacaggtgc cgcgcgccag caaaatcact
    29341 tttttgcgtt catagcgatc cgccagcaca ccgccaacca tcaggccaac aaacatcgcg
    29401 ccgccggtca gcgtcaccga aagccccacc tgccaggtag aatgcgtcat catctggatc
    29461 tgcaccggca ccgcgacgcc gagcaaaccc agagacacaa ttgagataaa acgagcgagg
    29521 aatactgcgc gaaacgccgg gtgcgttttc aacaggctga ggttaagcag ccaggattgt
    29581 ttattcatta caatgccttg ccatcaaata ttttaaatac ctattcttag gctgcacatg
    29641 ctaacatatc caaataagat cgataacgat aattaatttc attatcatgg aagttcgtat
    29701 gtctggttct gttgccgtga cacgcgccat tgccgtgccc ggattgctgt tattactgat
    29761 tatcgcgacg gcattaagcc tgctcattgg ggcaaaatca ctccccgctt ccgtagtgct
    29821 ggaggccctc tccggcacct gccagagcgc cgactgcacc atcgtgctcg acgcgcgact
    29881 gccgcgcacc cttgccggtt tacttgcagg cggcgcgctt ggccttgccg gggcgttgat
    29941 gcaaaccctc acccgaaatc cacttgccga ccccggcttg cttggcgtga atgccggagc
    30001 cagctttgcc attgtgctgg gtgcggcgct gtttggttac tcatccgcgc aggaacaact
    30061 ggcgatggcc ttcgccgggg cgctggtggc ctcattgatt gttgccttta ccggcagtca
    30121 gggcggcggg cagttaagtc cggtgcgttt aaccctggcg ggcgtggcgc tggcggcggt
    30181 gctggaagga ctgaccagcg gcatcgccct gcttaatcct gacgtctacg atcaactgcg
    30241 tttctggcaa gccggttcgc tggatattcg caatctacat accttaaaag tggtgctgat
    30301 cccggttctg atcgccggag caactgcgct attactgagt cgcgcgctga acagtttgag
    30361 cctcggcagt gacaccgcga cggcgctggg cagtcgcgtg gcgcgcacac agttaattgg
    30421 tctgctggcg attaccgtgc tttgtggtag tgcgacagcg gtagttggcc cgattgcctt
    30481 tattggcctg atgatgccgc atatggcgcg ttggctggtg ggtgccgatc atcgctggtc
    30541 gctgcccgtc acgctactcg ccacccctgc cctgctgctg tttgccgata tcatcgggcg
    30601 ggtgattgtg cccggcgagt tgcgcgtttc tgtggtcagt gcgtttattg gtgcaccggt
    30661 gctgatcttc ctcgtgcgac gtaaaacgcg aggtggtgca tgatttacgt ctctcgccga
    30721 ttactcatca cctgtttgct gcttatttcc gcctgtgtgg ttgcaggtat ctggggatta
    30781 cgcagcggtg ccgtcacgct ggaaacctcg caggtattcg ccgcgctgat gggcgatgcg
    30841 ccgcgcagta tgacgatggt ggtcaccgaa tggcgtttac cacgcgtgct gatggcgctg
    30901 ttgattggcg cggcactggg cgtcagcggt gcgatttttc agtcgctgat gcgtaacccg
    30961 ctcggcagcc ctgacgtaat gggctttaac accggggcgt ggagcggcgt gctggtggcg
    31021 atggtgctgt ttggtcagga cctgacggct atcgcgctgg cagcaatggt gggcggcatt
    31081 gtcacttctc tgttggtctg gctgctcgcc tggcgtaacg gtatcgacac ctttcggttg
    31141 attattatcg gcatcggcgt tcgcgccatg ctggtggcct ttaatacctg gctgttgctg
    31201 aaagcgtctt tagaaacggc gctaacagcg ggtttgtgga atgccggatc gctcaatggc
    31261 ctgacgtggg caaaaacttc gccttccgca cccatcatta tattgatgct cattgccgcc
    31321 gccttgctgg tgcggcaaat gcgcttgctg gagatgggcg atgataccgc ctgcgcgctg
    31381 ggcgtcagcg tcgaacgttc gcgtctgtta atgatgctgg ttgcagtggt gcttaccgct
    31441 gcggcaacag cgcttgccgg gccgatttcc tttattgctt tagtcgcgcc gcacatttcc
    31501 cgacgcatta gcggcaccgc tcgctggggg ctaacccagg cggcgctatg cggggcgctg
    31561 ttactgctgg cggccgatct ctgcgcccaa caactgttta tgccgtatca acttccggtt
    31621 ggcgtcgtta ccgtcagcct cggcggtatt taccttatcg tcttgttaat tcaggagtct
    31681 cgcaaaaaat gaccgaatca gtagcccgtt tgcgcggcga acagttaacc ctgggatatg
    31741 gcaaatatac cgttgcggaa aatctgactg tagaaattcc tgatggtcac ttcacggcaa
    31801 ttatcggacc aaatggctgt ggtaaatcca cgttactgcg taccttaagc cgcctgatga
    31861 cgcctgctca cgggcatgtc tggctggatg gcgagcacat tcaacattac gccagtaaag
    31921 aagttgcacg ccggattggt ctgttggcgc aaaatgctac cacgccgggc gatatcaccg
    31981 tgcaggagct ggtggcgcgt ggacgttatc cgcatcaacc gctgtttacc cgctggcgca
    32041 aagaggatga agaagcggta acgaaagcga tgcaggccac gggaataact catctggcag
    32101 atcaaagcgt ggataccctt tctggcggac aacgccagcg agcgtggatc gcgatggtgc
    32161 tggcccagga aacggcaatt atgctgctcg acgaacccac gacctggctg gatatcagtc
    32221 atcagattga tctactggaa ctgttaagcg aactgaaccg cgagaaaggc tataccctgg
    32281 cggcggtgct gcacgatctt aatcaggcct gtcgttacgc cagccatttg attgcattgc
    32341 gggaagggaa aattgttgct cagggagcgc cgaaggagat tgtcactgct gaactgattg
    32401 agcgcattta tggtctgcga tgcatgatca ttgacgatcc ggtagccgga acgccgcttg
    32461 tggtgccgct cggacgaacg gcaccttcaa ccgcaaatag ttaaactaag tggtctgcca
    32521 tcatggcatc ctgttttctg gatgccatcg catagcgcaa taacacgcta ccacaagcca
    32581 ccatcccgcc gatcagcgcg gaaaggatca caataatcgc cttacccgga ccgtcttttt
    32641 tcactggcaa tgacgggctt aactgatatt taaacggcgt aaaattcaca tcgttgatat
    32701 ttgcttttgt taactgctca acaagatact gacgattacg taattcaccg ttcagttccg
    32761 caacgtcagt gaccgctttt tcaatttcca gtttgcgttc aataccgtct gcgccgagag
    32821 aaatagagaa atcggggtca tctttaacgg cctgaccatt actgtatacg ggctttttaa
    32881 ttcccgccgc gttggcaatg tcgagtgaat aattgaggcg ctgaatgttt gcatcaagtt
    32941 gatttttcgt tttaatgcga tcctgagcca gtttttcttt ttcaaactgg gttttgatct
    33001 ccagtttatt acggacgttt tctatcgact ctttcaccac caacgcagag atataatcga
    33061 tatacccgct caaaacggtc tgcgcctctt tactggttgg cgcggtaaaa cttagcgtcc
    33121 aggaggtata cagtgacggt tcatcttttt tcttactggc attgtcatca accgctttca
    33181 ttttttcgct caatgcaaca atagcgcgat gcaaatccag ttcatcgatt ttcgcctctt
    33241 ttaattggtc catcacataa ggtgatgaac gcaggtactc ttccagcaag ctaacagact
    33301 gaaacttctt gataaacagg ttaaatgcac cgccacggtc gatattaaca tcgagatcaa
    33361 gaacgcggag tttggtaaag gttttctcca ggtcctgcca ctgaatggct tcggcgggag
    33421 taataacggc tgagctggtc catttttgcg gcaggatgaa agagatcagt aaacctgcgc
    33481 aggcaaatgc aaaaacgacc gccatgaccg tttttttggc ccgccataaa acttcgatta
    33541 gattaagtaa atcaatttca ttattactgg gcgacgccag aggatactca gaaaaatgag
    33601 cgtcacttcc ctgtttaata ttcaatgatg acatggggta taacctgaaa atgagtccaa
    33661 taggggaaaa tctccacggc gctcacttta ccagttggtg agaattatcc gaagctgaag
    33721 tttgtaaatt ccttccact
//
LOCUS       DAZGVQ010000036        33531 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000036 DAZGVQ010000000
VERSION     DAZGVQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.036"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(153..1337)
                     /locus_tag="ACHLAG_001706"
     CDS             complement(153..1337)
                     /locus_tag="ACHLAG_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0654954.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQISLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
     gene            complement(1637..1731)
                     /locus_tag="ACHLAG_001707"
     tRNA            complement(1637..1731)
                     /locus_tag="ACHLAG_001707"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1695..1697),aa:Sec,seq:tca)
     gene            2024..3406
                     /locus_tag="ACHLAG_001708"
     CDS             2024..3406
                     /locus_tag="ACHLAG_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFP0654955.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3416..5734
                     /gene="yicI"
                     /locus_tag="ACHLAG_001709"
     CDS             3416..5734
                     /gene="yicI"
                     /locus_tag="ACHLAG_001709"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFP0654956.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(5787..7496)
                     /locus_tag="ACHLAG_001710"
     CDS             complement(5787..7496)
                     /locus_tag="ACHLAG_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP0654957.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINTEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(7617..9008)
                     /gene="xanP"
                     /locus_tag="ACHLAG_001711"
     CDS             complement(7617..9008)
                     /gene="xanP"
                     /locus_tag="ACHLAG_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFP0654958.1"
                     /translation="MSVSTLESENAEPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            9288..10493
                     /gene="gltS"
                     /locus_tag="ACHLAG_001712"
     CDS             9288..10493
                     /gene="gltS"
                     /locus_tag="ACHLAG_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFP0654959.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            10496..11362
                     /locus_tag="ACHLAG_001713"
     CDS             10496..11362
                     /locus_tag="ACHLAG_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0654960.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISVWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIHSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(11347..13428)
                     /gene="recG"
                     /locus_tag="ACHLAG_001714"
     CDS             complement(11347..13428)
                     /gene="recG"
                     /locus_tag="ACHLAG_001714"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFP0654961.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPVEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(13434..14123)
                     /gene="trmH"
                     /locus_tag="ACHLAG_001715"
     CDS             complement(13434..14123)
                     /gene="trmH"
                     /locus_tag="ACHLAG_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFP0654962.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(14130..16238)
                     /gene="spoT"
                     /locus_tag="ACHLAG_001716"
     CDS             complement(14130..16238)
                     /gene="spoT"
                     /locus_tag="ACHLAG_001716"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFP0654963.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVPKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(16257..16532)
                     /gene="rpoZ"
                     /locus_tag="ACHLAG_001717"
     CDS             complement(16257..16532)
                     /gene="rpoZ"
                     /locus_tag="ACHLAG_001717"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP0654964.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(16587..17210)
                     /gene="gmk"
                     /locus_tag="ACHLAG_001718"
     CDS             complement(16587..17210)
                     /gene="gmk"
                     /locus_tag="ACHLAG_001718"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP0654965.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            17468..19150
                     /gene="ligB"
                     /locus_tag="ACHLAG_001719"
     CDS             17468..19150
                     /gene="ligB"
                     /locus_tag="ACHLAG_001719"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFP0654966.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEDISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNARDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(19147..19764)
                     /locus_tag="ACHLAG_001720"
     CDS             complement(19147..19764)
                     /locus_tag="ACHLAG_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFP0654967.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(20056..20880)
                     /gene="dinD"
                     /locus_tag="ACHLAG_001721"
     CDS             complement(20056..20880)
                     /gene="dinD"
                     /locus_tag="ACHLAG_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFP0654968.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(21101..21964)
                     /locus_tag="ACHLAG_001722"
     CDS             complement(21101..21964)
                     /locus_tag="ACHLAG_001722"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFP0654969.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            22091..22807
                     /gene="rph"
                     /locus_tag="ACHLAG_001723"
     CDS             22091..22807
                     /gene="rph"
                     /locus_tag="ACHLAG_001723"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFP0654970.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            22873..23514
                     /gene="pyrE"
                     /locus_tag="ACHLAG_001724"
     CDS             22873..23514
                     /gene="pyrE"
                     /locus_tag="ACHLAG_001724"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFP0654971.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(23551..24147)
                     /gene="slmA"
                     /locus_tag="ACHLAG_001725"
     CDS             complement(23551..24147)
                     /gene="slmA"
                     /locus_tag="ACHLAG_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFP0654972.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(24254..24712)
                     /gene="dut"
                     /locus_tag="ACHLAG_001726"
     CDS             complement(24254..24712)
                     /gene="dut"
                     /locus_tag="ACHLAG_001726"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFP0654973.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(24690..25910)
                     /gene="coaBC"
                     /locus_tag="ACHLAG_001727"
     CDS             complement(24690..25910)
                     /gene="coaBC"
                     /locus_tag="ACHLAG_001727"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFP0654974.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            26082..26750
                     /gene="radC"
                     /locus_tag="ACHLAG_001728"
     CDS             26082..26750
                     /gene="radC"
                     /locus_tag="ACHLAG_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP0654975.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHWVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            26967..27203
                     /gene="rpmB"
                     /locus_tag="ACHLAG_001729"
     CDS             26967..27203
                     /gene="rpmB"
                     /locus_tag="ACHLAG_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFP0654976.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            27224..27391
                     /gene="rpmG"
                     /locus_tag="ACHLAG_001730"
     CDS             27224..27391
                     /gene="rpmG"
                     /locus_tag="ACHLAG_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP0654977.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            27489..28298
                     /gene="mutM"
                     /locus_tag="ACHLAG_001731"
     CDS             27489..28298
                     /gene="mutM"
                     /locus_tag="ACHLAG_001731"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFP0654978.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(28337..28816)
                     /gene="coaD"
                     /locus_tag="ACHLAG_001732"
     CDS             complement(28337..28816)
                     /gene="coaD"
                     /locus_tag="ACHLAG_001732"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFP0654979.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPIKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(28824..30101)
                     /gene="waaA"
                     /locus_tag="ACHLAG_001733"
     CDS             complement(28824..30101)
                     /gene="waaA"
                     /locus_tag="ACHLAG_001733"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFP0654980.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            30514..31572
                     /gene="rfaQ"
                     /locus_tag="ACHLAG_001734"
     CDS             30514..31572
                     /gene="rfaQ"
                     /locus_tag="ACHLAG_001734"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFP0654981.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            31569..32693
                     /locus_tag="ACHLAG_001735"
     CDS             31569..32693
                     /locus_tag="ACHLAG_001735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP0654982.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            32686..33483
                     /gene="rfaP"
                     /locus_tag="ACHLAG_001736"
     CDS             32686..33483
                     /gene="rfaP"
                     /locus_tag="ACHLAG_001736"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFP0654983.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYSADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         7948 a         8288 c         8922 g         8373 t
ORIGIN      
        1 ccaatttaaa aatgtcaatt tgtttttaat tatgtttttg taataagttt gaagacgcaa
       61 aaaaatgata taataacaaa aatcctccag ccttgtaggc cagaggataa aatacttaat
      121 tgaggaaaac atcagggaaa agtaattctg acttaacttt cccggctgga gagtgaatca
      181 atgtaatcag cgtaccattg catcatttca cggcggccat cgagatacag ggcatgatta
      241 taggtacccc tgatggaatt tttatccaca tgagctaact gcatttcaat ccaggcagag
      301 ttaaagcctt gttcatgcag aattgtactc atggtgtgtc tgaaaccgtg ccctgtgagt
      361 cttcccttat atccaagtaa ttcaattact tgattaatgc tttccttact aattggtttg
      421 cggggatcat ttctaccgat aaagactagg ggatgatgtt ttgagattgg ttcaagctgc
      481 ttaaatagca taataacctg ctcagagagc ggcacaatat gaggtcgctt catcttcatt
      541 acttctgccg ggatctccca caattttgtc tcaaaatcga tatcttccca gcgtgcaaaa
      601 cgcagttctt gtgttcgtac gccggttaac ataatgatct gagtagctgt cttagtgatt
      661 atacttccgg tataacccgc cagatcgttg aggaagtggg gaagttcatt agcagtaaga
      721 aagggaaaat gtactttttt cggtgtagct aaagcactgg caagatcggg ggctgggtta
      781 tagtctgccc ggccagtaac aattgcatag cggaacacct caccacagcg ctggcggacc
      841 ttgcgcattt tctccagagc tccacgtttt tccattttac gcagtgcttc cagtaactcc
      901 attggtttga tttcggcaat agggcgcttg ccaatgtagg gaaaaatgtc tttttcaaag
      961 gtatcaatga tttcatcacg atagcgtaat gaccaccgat cggcttttga ctggtgccat
     1021 tcgcgagcaa tagcctcgaa cgtatttttc agacttattt gctgcgcgat tttttcttcc
     1081 ttttttgcct cccctggatc gcctccagca gcgaggagtt tcctggcttc actgcgtttc
     1141 tcgcgagcat cggctagcgt tacatcgggg tatacgccaa tagaaagcat cttttcttta
     1201 ccagcaaagc ggtacttcat gcgccagtat ttagaaccat tagtgttaat cagtagatac
     1261 ataccgccgc catcagcaag cttatagggt ttgtctttgg gttttgcggt tttcactttg
     1321 atatcggtta atgccatgac atcctccggc ttttgatgaa ttcatgttcc tgagggggat
     1381 ttctgccagt aactaacctg atacccccaa tacacccctt gtataacaaa gatgttgtta
     1441 aaagccgttg ttatgtatca catttttcct attatggggg tgcttgtatt tcaataggaa
     1501 ataacacccc ctaaagtacc cccaaaagaa acttgatacg gggtgaagta ggttgacctc
     1561 aggatacaaa taaatactga tatacaaggg attagaagag atttattgac attgggagag
     1621 ttcagaagag gtaaactggc ggaagatcac aggagtcgaa cctgcccggg accgctggcg
     1681 gccccaactg gatttgaagt ccagccgcct caccggagac gacgatcttc cgcgcctcga
     1741 ttgctacatg gaggcggggc gcattatagc tacttccttg agtttctaca tcccccagat
     1801 cagttttttc cgcctaatac acgcagcgta ttatcacgaa agcattatag ttcatataaa
     1861 acccatattt tacaatgata tatgagcgga attaacgcta ccacgatcac ataaaacaag
     1921 aatcataaat taataaccag atatcggaat attcgctctc acagggatgg tttacaaaat
     1981 gcgttatcgg tctcagcacc cctatagcat caaggaaaag cagatgaaga gtgaagtgtt
     2041 gtccgttaaa gagaaaattg gttatggcat gggagacgcc gccagccaca ttattttcga
     2101 taacgtaatg ttatatatga tgttctttta taccgatatt tttggcattc ctgccggttt
     2161 tgtcggaacc atgtttttgg tcgctcgtgc actggatgcg atttccgatc cttgcatggg
     2221 gttgttggcc gatcgaacgc gctctcgctg gggtaaattt cgtccgtggg tactgtttgg
     2281 cgcactgcca ttcgggatcg tctgtgtact ggcctatagc acgccagatc tcagtatgaa
     2341 cggcaaaatg atctatgcag caattactta caccctactt accttacttt ataccgtcgt
     2401 caatatccct tactgcgcat tgggtggtgt aatcaccaat gacccgactc agcgtatctc
     2461 gctgcaatcc tggcgttttg tgctggcgac cgcgggaggc atgctttcta ctgttctgat
     2521 gatgccactg gttaatttaa ttggcggtga taataaacca ctcggtttcc agggcggtat
     2581 cgcggtcctt tccgtggtgg cattcatgat gctggcattt tgtttcttca ccactaaaga
     2641 acgcgttgaa gcaccaccta caacaacgtc tatgcgggaa gatttacgtg atatctggca
     2701 aaacgaccag tggcggattg tcggtttact aaccattttc aatatcctgg cggtgtgcgt
     2761 acgcggtggg gcgatgatgt attacgtcac atggattttg ggcacgccgg aagtgtttgt
     2821 cgcttttctc accacttatt gcgtgggtaa cctgattggt tccgcactgg caaaacctct
     2881 gaccgactgg aaatgtaaag tcactatctt ctggtggacg aacgccctgc tggcagtgat
     2941 tagcctcgcg atgttctttg ttcccatgca ggccagcatc actatgtttg tcttcatctt
     3001 cgtgattggt gtgttgcatc aactggtgac acctatccag tgggtaatga tgtccgatac
     3061 cgtcgactac ggcgagtggt gcaatggtaa acgcctgacc gggatcagtt ttgctggcac
     3121 gctgtttgtg ctcaaactgg ggttggcctt cggcggcgct cttatcggct ggatgctggc
     3181 ttatggcgga tatgatgcgg cagaaaaagc gcagaacagc gccacgatta gcatcattat
     3241 tgcgctattc acgattgttc cggcgatctg ttatttgctg agcgcgatta tcgctaaacg
     3301 ctactactca ctcacgacgc acaatctgaa aaccgttatg gaacagctgg ctcagggtaa
     3361 acgccgttgc cagcaacaat tcacctctca agaagtgcag aactaaggaa cggcaatgaa
     3421 aattagcgat ggaaactggt tgattcaacc tggcctcaat ttgattcacc cgcttcaggt
     3481 gttcgaggtt gaacagcagg ataatgaaat ggtggtctat gctgcccccc gtgatgtgcg
     3541 tgaacgtacc tggcagcttg atacgccttt atttacgttg cgctttttct ccccacagga
     3601 aggtattgtc ggtgtgcgga ttgagcattt tcagggggcg ctgaataacg gtcctcatta
     3661 tccgctcaat attttgcagg acgtgaaggt cacaatcgaa aacacagaac gttatgctga
     3721 gtttaaaagt ggcaacttaa gcgcgcgtgt cagcaaaggt gagttctggt cactggattt
     3781 tctgcgcaac ggcgaacgta ttaccggtag tcaggtgaaa aataatggct acgtgcagga
     3841 cacgaataat caacgcaatt atatgtttga gcggcttgat cttggcgttg gcgaaacagt
     3901 ttacggtctg ggagagcgct ttactgccct ggtgcgcaat ggccagacgg tagagacctg
     3961 gaaccgggac ggcggcacaa gtactgaaca ggcgtataaa aatatcccgt tctacatgac
     4021 taaccgtggt tatggggtac tggtcaatca tccccagtgt gtctcttttg aagtgggatc
     4081 ggagaaagtc tccaaagtgc agttcagcgt tgagagtgaa tatctcgaat actttgttat
     4141 cgacggcccg acgccgaaag cggtacttga tcgttatacc cgctttactg gtcgtccggc
     4201 gctgccgccc gcgtggtcct tcggcctgtg gctaaccact tcatttacca ccaactacga
     4261 cgaagcgacg gtaaacagct ttatcgatgg tatggcggaa cgcaatctgc cgctgcatgt
     4321 tttccacttt gactgtttct ggatgaaagc cttccagtgg tgcgattttg agtgggaccc
     4381 gctgactttc cctgacccgg aagggatgat ccgccgcctg aaagcgaaag ggctgaaaat
     4441 ctgcgtctgg attaacccct atatcggtca aaaatccccc gtctttaaag agttacaaga
     4501 gaaaggctat ttactcaaac gccccgacgg ttcgctatgg cagtgggata aatggcagcc
     4561 aggtctggcg atttatgact ttaccaatcc ggatgcctgc aaatggtacg ccgacaaact
     4621 gaaaggtctg gtcgcgatgg gcgttgattg ctttaagacc gactttggcg aacgtatccc
     4681 aactgatgtt cagtggtttg acggttccga tccgcagaaa atgcataacc attatgcgta
     4741 catctacaac gaactggtgt ggaacgtgct caaggacacc gttggtgagg aagaagctgt
     4801 cttgtttgcc cgctcggcct ccgtcggtgc gcagaaattc ccggtacact ggggtggcga
     4861 ttgttacgct aactacgaat caatggcgga aagcctgcgc ggtggtttgt ctattggcct
     4921 ttcaggtttt ggcttctgga gccacgatat cggcggcttt gaaaataccg ctccggcgca
     4981 cgtttacaaa cgctggtgcg cgtttggttt gctctccagc catagccgtt tacacggtag
     5041 caaatcttat cgtgtgccgt gggcttacga tgatgagtcc tgtgatgtgg tgcgcttctt
     5101 cacgcaactg aaatgccgca tgatgccgta tctgtatcgt gaagctgcgc gtgcgaacgc
     5161 gcggggtacg ccgatgatgc gggccatgat gatggagttc ccggacgatc cggcttgtga
     5221 ttaccttgac cgtcaataca tgttaggcga caacgtgatg gttgcgccgg tgttcactga
     5281 agcgggcgat gtgcagttct acctgccgga aggtcgctgg acacacctgt ggcacaacga
     5341 tgaactcgac ggtagtcgct ggcataaaca gcagcacggc ttcctgagtc tgcccgttta
     5401 tgtgcgtgat aacactctac tggcgctggg caacaacgat caacgtcccg attacgtgtg
     5461 gcacgaaggc acggcattcc acctctttaa tctgcaagac gggcatgaag ccgtctgtga
     5521 agtgcccgct gctgacggat cggtgatctt tactttaaaa gcagcacgta ctggcaacac
     5581 gattactgtg actggtgcgg gcgaggcgaa gaactggaca ctgtgcctgc gcaatgttgt
     5641 gaaagtaaat ggtctgcaag acggttcgca ggctgaaagt gagcaggggc tggtggtgaa
     5701 gcctcaaggg aatgcgctga caattacgtt gtaatctatt gatggaatgc cgggtacgtc
     5761 tgagtcgtac ccggcgagat tgatgtttac tgcaccagct ctgccgacgt aacttcaccg
     5821 ctggaaacga tgccagcatt catggtttga gtcacttgct gctttgcggc gttcacggca
     5881 tgcaattgcc cgctaaccgt aggtttcagc ggggcattgg cctgaatatc tccactggcg
     5941 gtaagctgaa tattgccgtc gccagtcaac ggtaattcag gccatcccca ttgttgcaaa
     6001 atatttaccg gcacaccgcg tccattcagg ctgatatggg tctgacgttg tggcgtttgt
     6061 gaaacactgg cagtggcttc cagaatgcct ttttcagtaa atgcactcag ttcgctgata
     6121 ttcaccgtgc tgctgttggc ggtcagcgcc agcgacgggc gacgaacatc aacacgattg
     6181 aatgtcgcgg cggcggcatt cagattcgcc gagccactcc agacgcccca tttatgatcg
     6241 gtaaccagcg tcaggttggc accgtaacca tcgagcgcgg tgagctgcca cgggaagtca
     6301 gggtcgatat caatgatcag attgcggctg gcgctaaatc tcttcagttg caggctgttt
     6361 aaccaaccgg gtgtcgtttc catccacaac tgttgccagt ttttcggcaa ggtatattcc
     6421 agcccggcaa ttgccgcatc atcaaggatc aacgttttcc cgtcacgcag ccagttccct
     6481 gacgttctga ccataccccc ttcccagcgg ctggtgaact ggcgcagcgc tacgccctgc
     6541 ggggaaaatt ccgtgtttat aatcgggtca aataaatgca gcgaaccata aatgaactcg
     6601 ctagcgttca tcgacagttt gccttcctgt gtctgccagt catctttact gaaggtcatg
     6661 ttgcgcaagc tgagatcgag gtcggtcacc gcccagtccg gaccttgcaa acgagcatcg
     6721 atcacttcca ggcgaccaat ctgcaacgac gggacagagc gtaatggcgc aaagaagtcg
     6781 gttagcgatt tttcgctttg tagacgtata tccgccatgc gcaggttttc cacttgccag
     6841 ctgccgtcgg cgttacgctg cgcgtttccg gttaatgtcc cgcgggcgat gtcggcaccc
     6901 aggttagtca gcgtaacgcg atcgttatca atactgcctt caatcagtac attggtggca
     6961 ggaacatcat tgagcgaaag cgatccggca ctaaactgaa tctgcgcctt cgtacccagc
     7021 actttaccgg cttttggtga ccacggaact acgccgccat ttacccgctg cgcgctcagt
     7081 ttccattcgc tattcgggct attaaacgcc atatcacgca gttgcagacg atcggctttg
     7141 aacggtagcg gcgcggtctg gtcggtgaga ttcagcgtcc cgttttccag caggatggta
     7201 tcgacatggc gtggttcggt cagttgccga ctgcttagcg caatgtcgac actttttgcc
     7261 accagggtcg cgggctggcc atcacggcca aacgtgacgt tctccagcac gatatgagat
     7321 ggcgcggaaa aacggtgatc catcgccccg aaggccagat gatagtcgct attctcggaa
     7381 acccatgcgc tgatatgttc tgctccccag cgggtttgca gaagaaaata gaggccagcg
     7441 atcaccacta acagagcgat gagaatgtag agaagcagct tcccaataaa tttcatggtc
     7501 ttccatcccg tgaaatgcac ataagggagt tatgcacgat ttacgcgcaa tcctcaaggc
     7561 gggaatggtg aaagaggtga gactgcggcg gtaattatct taccgccgcc agtgaattac
     7621 tgtttttctg gtgggaaaat cagattcagc acgatggcag taataccgcc cgcggcgatc
     7681 ccggaggaga gcaggttttt cagccattca ggggcaaact gcaaaatcag cggctgctga
     7741 gacacgccca gaccaaccgc cagcgacagc gcgataatca gaatcgcccg acggttcagc
     7801 ggttcacgag aaacgatacg cacaccggag gcggcgatag tgccaaacat tacaagcgtt
     7861 gcgccgccca gaaccggttc tggaatgtgt tgtacaaaac cgctcactgc cgggaacaga
     7921 cccagcacga tcaacatcag cgcgacgaca aaaccgacat agcggctggc aacaccagtc
     7981 aactggatca ctccgttgtt ttgcccgaag caggagttcg ggaaggtgtt aaacaccgcc
     8041 gaaacaaacg agttcaggcc gtttgccagc acgccgcctt tcaggcgttt catgtacagc
     8101 ggaccggaca ctggctgttc ggaaacgtca gaggtcgccg tgatatcgcc aatggtttcc
     8161 agcgaagtga tcataaagac cagcatcagc ggcagcagca gactccattc aatgccaaga
     8221 ccgtaataga gcggcgttgg caccataatc agttcttgcg tcatcggttc gttgctttct
     8281 ggcaacatgc ccataaacca cgccagcgca tatccggccg ccatcgcaat caccagtgag
     8341 gccacgcgta agtaagggtt acgttgacgg ttaagcagga taattaaggc taagaccacg
     8401 cctgccagca gcagattttt cggtgcgccg aaggtgttat cgctcatggc tgcgtaaccg
     8461 ccgccaatgg acgttaaccc aacctgaatt agcgacaggc cgataatcat caccacaacg
     8521 ccagaaacca gcggcgtaat aatgcggcgc gccagatgca gaacgcggga gatcaccatc
     8581 tcggtgcaac ttgccagcat caacgtgccg aacaaagccg ccatcatggt aggaacatca
     8641 gcaccaccgg ttttcagcgc ggtaccgccc ataatcagcg gggcaacaaa gttgaagctg
     8701 gtgccctgaa tagacaacag cccggagcca accggacccc aggccttaat ttgaataatc
     8761 gatgccacac cggaggcaaa caacgacatg ctaataatgt gttgcgtgtc ttgtgccggt
     8821 aaacccagcg cctggcagat taatagcgct ggcgtgatca ccgcaacgaa catcgccagc
     8881 agatgctgac aggcggcaaa cagggtttga ggaagcggcg gacgatcttc aagacggtaa
     8941 atcagttcgc tgttttgagt ctgcgcaacc ggttccgcat tttctgactc gagggtggaa
     9001 acagacatcg aaaacaatcc cgtggtagca aagcgggcat tttagctgac cgaaagagaa
     9061 aagcaaacgt ttgccacatc ctcatctaaa tatttaaatg agataaatgc acttttttat
     9121 ataacttttg gtatttttct gcttaaaatc catgccattt gttcattaac agctgagaat
     9181 ttgacccagg aaagcataac tcgatacccg tcatagttca tgaagttgcc actgcgcgat
     9241 gaagtatgac gagtatgaaa gagtgatgcg gatacaaagg agtaactatg tttcatctcg
     9301 atactttagc aacgcttgtt gccgcaacgc tgacgttgct gctcgggcgt aagttggtcc
     9361 attccgtctc ctttttgaag aaatacacca taccggaacc tgttgcgggt ggtttgttgg
     9421 tggcgctggc gctactagta ctgaaaaaaa gcatgggctg ggaagtcaac tttgatatgt
     9481 ccctgcgcga tccgttaatg ctggctttct tcgccaccat tggcctgaac gccaacattg
     9541 ccagtttgcg tgccggtggg cgtgtggttg gcatcttctt gattgtggtt gttggtctgt
     9601 tggtgatgca aaatgccatt ggcattggta tggctagcct gttagggctt gatccgctga
     9661 tggggctgtt ggccggttct attacgcttt ccggcggtca cggtacgggc gctgcgtgga
     9721 gtaaattgtt cattgaacgt tatggcttca ccaatgcgac ggaagtggcg atggcctgtg
     9781 caacgttcgg tctggtgctg ggcggcttga ttggcggtcc ggtggcgcgc tatctggtga
     9841 aacactccac cacgccgaac ggtattccgg atgaccagga agtcccgacg gcgtttgaaa
     9901 agccggatgt gggacgcatg atcacctcgt tggtgctgat tgaaactatc gcgctgattg
     9961 ctatctgcct gacggtgggg aaaattgttg cgcaactttt ggctggcact gcttttgaac
    10021 tgccgacctt cgtctgtgtc ctgtttgttg gcgtgattct gagcaacggt ctgtcaatga
    10081 tgggctttta ccgcgtcttt gagcgtgcgg tatccgtgct gggtaacgta agcctgtcgt
    10141 tgttcctggc gatggcgctg atggggctga aactgtggga gctggcttcg ctggcgctgc
    10201 cgatgctggc gattctggtg gtacagacca tcttcatggc gttgtatgcc atcttcgtta
    10261 cctggcgcat gatgggcaaa aactacgatg cggcagtgct ggctgcgggt cactgtggtt
    10321 ttggcctcgg tgcaacgcca acggcaatcg ccaacatgca ggcgatcact gaacgctttg
    10381 gcccgtcgca catggcgttt ttggtggtgc cgatggtcgg tgcgttcttt atcgatatcg
    10441 tcaatgcgct ggtgattaag ctgtatttaa tgttgccgat ttttgccggt taaaaatgaa
    10501 aaagcgtcgg aaggaaccgg aaacgctacg tgaacactgt cgccatattt ttggtgatga
    10561 gccgccagtg ctctgtgtat gggaaacgga atttgattac gcagatgccg aactcaaggc
    10621 gctggcggct aaagagtggc agcagatcag cgtatgggat ttgagtgctt actatgtact
    10681 caatctcgtt tataacgaac cgatgcaaat agagttgttt cgctatctgt ttccgctctg
    10741 cctggcgcaa tggcatgaaa ccgttttagc tggcggttat ggtgatcact tcgaagaatc
    10801 cttaatgaag gcattgtgtc ggccctatct ctggcaggag atgatgaatg cttcccaacg
    10861 ccagcaggta cggcaatttt tactggacac cgcgttgcag cgtatggata acgagcgtgg
    10921 tttcaataac gttctttgct ggctggcggt gttcaatact ctgggtgggg ccgcgccgtt
    10981 aattcattct ctctggtcgc gctggtgggc gctggatacg ccaggaaaag cggtgtgtgc
    11041 tattcagtat gccgcgcatc taatttatcc catagaagcc aatccgttat ggtcgcaaga
    11101 atggataggc tgggggcatc cattaggcca taaagatggc tggtcctctg ataatcgcgc
    11161 ttttcttcgc caaatgttaa cacctgaaat gattgtggct ggcgtacagg cagcggctga
    11221 aattctgcgt ggtgaaccgg aaggcgcaat ggcggcgcga attgcacagg atgcgtatga
    11281 ggcaatggat atcctcacta tccagattga agatttactg cgtgacttgt cttgtgatga
    11341 gtctggtcat gcgttggagt aacgttccgt ctccggcatc cagcgttcta tcagggcttt
    11401 tgcttgttgt gggtaacgtt cgtgaatatg tcgcgccagg cgctgaactt ccgggatcat
    11461 cgcctgatcg cgcagtaaat ccgccacttt aaactcagca ttacccgtct gacgcgtacc
    11521 taacagttca cccggtccgc gaatctctaa gtctttctgt gcaatcacaa aaccgtcgtt
    11581 gctgtcgcgc agcacttgca gccgaatttg cgcggttttg gagagcggcg ttttgtagag
    11641 cagcacgcag tgagaagcca ccgcaccacg acctacacgc ccgcgaagct ggtgtaactg
    11701 cgccagaccc agacgctccg ggttttcgat aatcatcaga ctggcgttag gcacatcaac
    11761 gccgacttca ataacggttg tggcaaccag caggtgtagc tcaccttgtt taaacgacgc
    11821 catcaccgcc tgtttctcga caggtttcat ccgcccgtgt accaggccaa cgttcaactc
    11881 tggtagcgcc agtttcaact cttcccaggt agcttccgcc gcctgcgctt ccagcaattc
    11941 cgactcttca atcaacgtac aaacccagta tgcctgacga ccttcagtta tgcaggcgtg
    12001 gcgcacgcgg tcaatgatgt cggtacggcg ggtatcagga atagcgaccg tagtcactgg
    12061 cgtgcggcct ggcggcagct catctatcac cgaggtatcg agatcggcat acgcagtcat
    12121 tgccagcgtg cgggggatcg gcgtggcggt catgatcaac tgatgcggat ggaagccctg
    12181 ctgctggcct ttctcccaca atgccagacg ctgatgcacg ccaaaacgat gctgttcgtc
    12241 gataatcacc agcgccaggc cgttaaactg cacctgttcc tggaagatgg cgtgtgtacc
    12301 gacaatcatc tgcacctgac cgctggcgat ggcttcctgc tgtgccagcc gtgctttacc
    12361 tttctgctta ccggcgagcc agcccacttc gataccgagc ggtgcaaacc agttgcggaa
    12421 gttattggcg tgctgctcgg cgagtaattc ggttggtgcc atcaatgcta cctgtttgcc
    12481 gtgggcaatc gcacgcaacg cggcgagggc ggcgaccagc gttttaccgg aacctacatc
    12541 gccctgcacc agacgcatca tcggcacatc cagcgccata tcgcgctcga tctccgccac
    12601 tacgcgtgcc tgtgcgcccg ttggcttgaa cggtaaggcg gcgaggagtt tatttttcag
    12661 cgtgtcattg gcgctcagcg gctgggcatg aaaacgctgt gctccggcac gtaaggctaa
    12721 catgctgagg ttgtgcgcca gcagttcttc cagaatcaga cgacgttgcg ccggatgctg
    12781 cccggtttcc agatcgctaa gctgtagcgt cggtggcggg cggtgcaaag tgcgcaacgc
    12841 ttccggtagc gtcatcattc cttgtgacag ttccggcggc aggagttctt caatggcgca
    12901 ggtgtcgagc agatccagcg cctggtcggt taatttacgc agcgtggcct gctttacgcc
    12961 ttccgttgtt ggataaaccg gcgtgagcgt ttcctgtaat tctggcgtgc tgagatcgcc
    13021 ctgcacgcgg tattccgggt ggatcatctc cgcaccatat ttaccgcgct ttgcttcgcc
    13081 ataagccagt acacggcggc ccgtcgccag gctatttttc attgccgcgc tgaaattgaa
    13141 aaagcgcatg gtgaggatgc cggaaccgtc gctgatctgg caggtcatca tccgccgacc
    13201 gccgaaggag atattgcagt tcagcacttc gccttccacc gtggcataaa cgcccggcag
    13261 tagttctccg atggggtaga gatgggtgcg atcttcgtag cgcaggggaa ggtgtaagag
    13321 tagatcctgc acggtatgca ggttgatttt cgccagtttg ttactaagtg ctgcgccaac
    13381 gcccgttagg gaactgagtg ggacagcatc taacaggcga cctttcatgg cacttaccct
    13441 gcagcctgca tagtagccca ccagtcggca tcagcttcga tctcgccttg ctgattgacg
    13501 tggggataag gcaggccttt gcgttttgcg actttcgcca gcaccggata gccgccttca
    13561 aacaacaggc gttgttgctc tgcttccggc aacatgctgt tttcacgcag gtacatgcct
    13621 gcattttgcc gctgacgctg ggcttcgtaa agaatgaggg ctgaggcaac ggaaacattc
    13681 agcgactgca ccatgccgat catcggaatg atgatgtcct gatccgccag ggccaatgct
    13741 tcctgcgtga tgcccgtttt ctcctgtccc atcaaaatgc aggtcgggcg agtgtaatca
    13801 atttcgcgga aatcgacagc gttatcagaa agatgggttg ccagaatctg catgccctgg
    13861 cctttgagat gagcgacggc atcgccaatg gtgcggtgtg ttttcacctg tacccagctg
    13921 ttactacccg ccgctgccga agccatggtg cgcatgcggc taccaggcca gacggcgtga
    13981 acttcatgta cgccaacggc atctgcggta cgaataatcg cagaaacgtt atgaggtttg
    14041 tggacctgct ccatgcagac ggtcagatca ggctgccgcc tggcgagcat ttcgcagatg
    14101 cgtgcataac gtgttgggtt cataaaacat taatttcggt ttcgggtgac tttaatcacg
    14161 tctggcatca cgcggatttt gcgcatgata ttcgccagat gcacacggtc acgagcggtc
    14221 agacgaataa aggcgctgta gacgcgacca tctttctctt ccgtattcaa actttgaata
    14281 ttcgaggtcg tggtgttaat tgccgccgtc aggtttgcca gcgcaccctg atgattgaac
    14341 atctccacct tgatttcggt gatgaactcc tgcgccgtct ctttatccca ttccacagcc
    14401 ataaacttct ctggctcttt ctggtagcca cggatgttac ggcaggattc atggtggatc
    14461 accagacctt taccggggct gacgtgggcg ataatcgggt cgccaggaat ggggcggcag
    14521 catttcgcaa aggtgatcag cacgccatca gcgcctttga tcggcagatg cccgtggctt
    14581 tgagtcgccg gtggaatgga ggcgtcccca tgttgtagat ttttcgcgac caccacgctc
    14641 attgcgttac caaggccgat ttctgccagc agatcgtcaa gcgttgccag cttcatgcga
    14701 tccagctcgc gctgaatatt ttcctgcggg atttcattca gcttacggct accacccaaa
    14761 gcatggttga gcagacgacg gcccaggctt acagaatcat cacgcttgag gtttttcagc
    14821 aactgacgaa ttttggcgcg cgcttttgag ctaacgacaa agttcagcca cgcggcattc
    14881 gggcgagcgc ccggtgcggt aatgatttca acggtttgac cgctggtaag cggctgcgac
    14941 agcgggtaag gctggcggtc aacgcgtgcg cccacgcagg catgaccgat atcggtatgc
    15001 actgcataag cgaagtcgac gggcgttgca ccggcaggca gctcgacaat tcgcccttct
    15061 ggtgtgaaaa cgtaaatctc atccgggaag agatcggatt taacgctctc gataaattca
    15121 aacgaactac cggcgctctg ttgcagctcc agcaggcttt gcatccagcg ctgggcgcgg
    15181 atttgtgcgg tagtactggt ttcgccgtgc tctttataag cccagtgcgc ggcaacaccc
    15241 atctccgcca tctgatccat atcttcggta cggatctgga cctcaaccgg cacgccgtgc
    15301 gggccaatca tcgaggtgtg caacgactga tagccgttcg ctttaggaat ggcgatatag
    15361 tctttcacgc gacccggacg cggcttgtac aggctatgca tttgcccgag cacgcgataa
    15421 caggtgtcag aatcattgac gatcacgcgg aaagcgtaga tgtccatgat tgagtgaaaa
    15481 cgctgctctt tgggcaccat tttgcagtaa atcgaataaa gatgcttttc gcgaccactg
    15541 acgcggcacg gtattcccgc ttcctgcaaa cgcccttcga tttcagagag gattttttgg
    15601 atcatctctt tacggttgcc gcgcgcggct ttcaccactt ctttaattac gcggtaacga
    15661 ttgggataca gcgcctcaaa acccagctct tcgagttcgg ttttaatgtg gtggatacct
    15721 aaacggtgcg ccagcgggct ataaatttcg agagtttcac gggcgatgcg gcgacgtttg
    15781 tccgggcgaa gtgagcccag cgtgcgcatg ttgtgggtac ggtcggcaag tttgatgaga
    15841 atgacgcgga tatcctgcac catcgccata atcatcttgc gaaagttttc ggcctgcgcc
    15901 tctttcttat cgcggaactt gagcttatca agtttcgaca ccccctctac cagctcggcg
    15961 acgcttttac caaaaagctg ttccatgtcc tggtaggtgg cgggagtatc ttcaatcacg
    16021 tcatgcagca gcgccgccat cagcgtttca tagtcgagtt tcatctcggc cagaatgcag
    16081 gcaaccgcta ccgggtgcgt gatatagggt tcaccgcttg aacgtgtttg cccctcgtga
    16141 gcatcacgtg caacgagata cgcctgccgc agacgcttga tttggtcttc cggcaggtag
    16201 gtttgaatca gttgattcag gctttcaaac agatacaagg gcgacccgct ttgtgattaa
    16261 cgacgacctt cagcaatagc ggtaacggct tgtaattcag cggcttcctg ctcttgctgt
    16321 tcctggcgtt cgcgaacgtc gaggatctgg ttgttgatca gaccttcttc gatttcgcgc
    16381 agcgcgatta cagtggtttt atcgttttct tccggtacca gcggatcctt tccgcctacc
    16441 tgcatctgac gagcgcgacg cgcggcgacc agtaccaggt caaaacggtt accaattttc
    16501 tctacagcgt cctgaacagt tacgcgtgcc atacttaaaa agctccacag gtgaagaaat
    16561 gactgggcat gatactgaaa tcaggttcag tctgccaaca atttgctgat taaagcgtca
    16621 tgacgctgct tttggcggct catgcgcaga cgttcggcgc gaataatggt cttcaaatcg
    16681 gtcaacgcgg tatcgaagtc atcattcaca atcagataat catattcggc gtaatggctc
    16741 atttctgcaa cagcttgcgc catacgcttt gcaatgacct cttcgctgtc ctgaccgcga
    16801 ccgcgtagac ggcggtccag ttcaattttg gacggcggta aaataaagat actccgcgcg
    16861 tgcggcattt tctggcgaat ttgctgcgcg ccctgccagt cgatatcgag aaaaacatcg
    16921 acaccggtcg ccagtacttg ctcaatggcc tcacgcgaag tgccatagta attaccaaaa
    16981 acttctgcgt gttcgaggaa cgcatctctg ctaatcattt ctttaaattc atcatgatta
    17041 acaaagaaat aatgttcacc gtggacttca ccaggacgcg gttggcgtgt ggtgtgtgaa
    17101 acagaaacct gggtgtcata caacggttgg gtttttaata aagcctgaat caggctggat
    17161 ttacccgcgc cactgggggc agaaacaata taaagcgtgc cttgagccat gagtatcttt
    17221 cgtatgtgat tagcgaaata aagcctacat acgagcttat tatacacggc gcggccatgt
    17281 gacgtagccc ttgctcacac tttttgcgca aagttgttgc gttttgcgta gctgtttccg
    17341 catttgcctg tgatttactg caataaagac gattctcagg aagcgtcctc gcagactccg
    17401 tttttgccac tcggctttgc cttcaccacg ttgtatatct gccagcctga ctacagcgga
    17461 ggtgaagatg aaagtatgga tggcgatatt aataagtatc ttgtgctggc aatcatctgt
    17521 gtgggcggtc tgtccggcct ggtcgccagc cagagcacag gaagatattt cccgcctgca
    17581 acagcaaata aaacagtggg acgatgacta ctggaaggaa ggaaaaagtg aggtggaaga
    17641 cggtgtttac gatcagttaa gcgcccgtct tacgcagtgg caacgctgct ttgggagcga
    17701 gccccgcgac gtgatgatgc cgcctttaaa tggtgcggtt atgcatccgg ttgcccatac
    17761 gggtgtacgc aaaatggtgg ataaaaacgc attaagtctg tggatgcgag aacgtagcga
    17821 tctttgggtg cagccaaaag ttgatggcgt tgcggtaacc ctggtttatc gggacgggaa
    17881 actgaacaaa gcaatcagtc gcggtaatgg cctgaaaggc gaggactgga cgcagaaagt
    17941 tagcttaatt tccgctgtgc cgcaaaccgt tagcgggcct ttagccaaca gtacgcttca
    18001 gggggaaata tttctccagc gcgaggggca tatccaacaa caaatggggg gaataaatgc
    18061 ccgcgcaaaa gttgctggcc tgatgatgcg ccaggacgat agcgacacgc tgaattctct
    18121 gggcgttttt gtctgggcat ggccggatgg accgcagtta atgaccgatc gtttaaagga
    18181 gctggctacc gcaggtttta ctctaacgca gaggtatacc cgtgcggtta aaaatgctga
    18241 tgaggttgcg cgcgtacgca atgagtggtg gaaagcgaaa ttacccttcg tcaccgatgg
    18301 cgtggttgtg cgaggggcga aagaacccga atcccgccat tggctaccag gccaggcaga
    18361 atggctggtg gcctggaaat atcaacctgt agctcaggtt gccgaagtga aggcgattca
    18421 gtttgcggtg ggtaagagcg gtaaaatatc ggtggttgcg tcactcgcac ctgtcatgct
    18481 ggatgataaa aaagtccagc gggtgaatat tggttccgtc aggcgctggc aggagtggga
    18541 tattgcgcct ggtgatcaga tcctcgtcag ccttgccggt cagggtattc ctcgcattga
    18601 tgatgtggtc tggcgcggtg cagaacgtac aaaaccgaca ccaccagaaa accgctttaa
    18661 ctcgttgacc tgctactttg cttctgatgt ttgtcaggaa cagttcattt cacgcttagt
    18721 ctggctgggg tcaaaacagg ttctggggct ggatggcatt ggtgaggccg gctggcgcgc
    18781 gctgcatcag actcatcgct ttgagcatat cttttcctgg cttttattaa cgccagagca
    18841 attacagaac acgccgggga tcgcgaaaag taaaagtgcg cagctatggc atcagtttaa
    18901 tctggctcgt aagcagcctt ttactcgctg ggtgatggca atgggaatac cgctaacccg
    18961 ggcggcgctt aatgcccgtg atgaacggtc ctggtcgcaa cttttattta gcacggagca
    19021 gttctggcag cagctgccgg ggactggatc ggggcgcgcc agacaggtta ttgaatggaa
    19081 ggaaaatgcg caaatcaaga agctgggcag ttggctggct gcccagcaga tcacaggttt
    19141 tgaaccttag tgtccttcgt ggctgtagta aaagaccggt aatcccagct taaggcgtag
    19201 cgccagtaag cgggcaaaaa agccgaatac cagggtggag ataattacca catcatggtt
    19261 agaaacatag tgttgcagcg cgatgtacag cacggcggag gcaaatgaga caccggcata
    19321 caactctttc tggaatacca gtgggatacg tttacagaac atatcgcgca aaacgccgcc
    19381 aaacacgccg gtggttaccg ccgcgacaac ggcaataatt gggccgtgcc ccatatccag
    19441 tgcaacctgt gcgccgatga tagaaaagac caccagtcca agcgcatcga gcaccagaaa
    19501 cactttgcgc aggtaaggca ttacaggagc aacgatagtg gtgagtactg cggcggtagc
    19561 gacgataatc acatattccg ggtgtttgac ccagccgagc ggatagtggc ccagcagaat
    19621 atcgcgcact gaccctccgc caattgcggt ggcggtcgca ataataatta cgccaaatgt
    19681 atccatgcgg cgtcgcccgg ccgccagagc ccccgtcatg gcttcggcag taatgccaac
    19741 caaatacaaa atgtgtaaca gcatggttct ctccctaaaa atctgtctgg cagggtagcg
    19801 atttgtgcgt attgtcacga ttgagatttt ttaaggtgag aggaaattga taacttgtag
    19861 ttatgtgaaa tcgctatttt ctgtagcgta gatgcattct tctgacttct tgtctgagaa
    19921 aatgcataag aaaaaatagg ttaaaattga ggggtggaaa agaaaggtgc aatgtttagc
    19981 cattgtgttc accgcacctg gactttggct tacgcatcgg gtatctcaat caacaaaaag
    20041 agtttttagt ttctgctatt tcttctctgt aatttttaca ctgttttcca actgcttgat
    20101 gctgacctgc ggggtcggca actcctcagg catggttccg ccaagttcct gaatggtttg
    20161 cctcaccttg ctacccacgt caaagtgtgt ggtattagct tgctgttttg aattcacgcc
    20221 atcccgcttg agtttttctt ctgtttgggt agctcgaaag agattagccg ccagttctgt
    20281 tgaacccata tgatccagga tcttctgact ctttttcagc cccttccgct gatggatggc
    20341 tttctgatct aatccaccat acagcccccg gtaaccatga ttctgaaata tggcgaaatc
    20401 gatagctgtc gctaccccag cttgctgcgc tgcctcaacc aattgtttgt tatgttcttt
    20461 caattcattt ctcagaaaaa gacgcttttc gtcttcacga agttgcctga aagcttcatc
    20521 gtcagccagc tcctgccgtc gggtctggat agcaaaataa gtttgccctg ccgcaataac
    20581 cggtttcgca gggtcgccgt tttgtactac caggtaacag gcatagcggg agagatgaac
    20641 atcttcaagt tctcgttgag cccctgaacc taatacaacc attttggtgg tttcaacgaa
    20701 atggtcagat gcagcttgat tgctggcttc gcacgcttga gtcgcgcggg ccagtacctt
    20761 ttgaaaattg cgccagtccc gataatccag taacggtgcc aattctctag ctgaccaaaa
    20821 ttcttgccct tcagtgccat aatgccgtat ttcttcaaaa ggttgatgat gttcgttcat
    20881 tgcccactcc atgtgatatc gattgcaaag aaagtaaatc tgtaactgta tttatataca
    20941 gttgtgagta tctcacgttc tcagtgtgct gggcaaaaaa acatcgcaaa atctggagca
    21001 ggcacgcaaa acagaattga agttgcgggg aagaaaaatg ccgctctgtt tgagtgggcg
    21061 gcatttgtga aggctgaaaa gtaagaattg gcgttacgag ttattcaatg ttctgaatct
    21121 gctcgcgcat ctgctcaatc aacactttca gctcaatcgc ggagtttgtc acttcggcat
    21181 tgatagactt cgacgcaaga gtgttcgact cgcggttgaa ttcctgcatc ataaagtcca
    21241 gacgacgacc aacggcttct tttttcttca gaatgttgta ggtctctttg acatgcgctt
    21301 ccaggcggtc cagttcttcg gcaacgtcaa ttcgttgtgc cagcagaacc agctcctgct
    21361 ccagacggtt gttttccaac tgaacctgag catcttccag cttcgcgacc agacgctcac
    21421 gctgccattg caggatttcc ggcatatggg cgcggacttt gaccacttcg gcggtgacgc
    21481 cttccagacg ctgctcgatc aatgctttca gtgcctgacc ttcggtttcg cgcgcgacaa
    21541 taaagtcgtc cagcgtacca tccagcgccg cgagaatttc agcggcaatg gcgtcaagat
    21601 cctgctcctg ggctgccatc acgcccggcc agcgtagaat atcaaccggg ttgatttccc
    21661 cttcgtcact ctgcattttt acccagttcg cggcagttac cagctgttta gccagttttt
    21721 cgttgaggat cagctcacct tgcgcgctaa catctggctc atagcgcagg gtacattcca
    21781 ctttaccgcg cgtcaggcga gaacgaatac gctcgcgaac gacaggttca aggctacgga
    21841 actgctccgg cagacgaaag taagtttcca gataacgctg gtttaccgag cgcatttccc
    21901 aggttgcgct cccccattca cccttgattt cacgccgggc gtaggcggtc atactgcgga
    21961 tcatagacgt tcctgtttat aaaaggagag gtggaaggat tatagccatc gatgccttgt
    22021 caggatagga ataaccgccg gaagtccgta taatgcgcag ccacatttgt ttcaagccgg
    22081 agatttcaat atgcgtccag caggccgtag caataatcag gtgcgtcccg ttaccctgac
    22141 tcgtaactat acaaaacatg cagaaggctc ggtgctggtc gaatttggcg ataccaaagt
    22201 gttgtgtacc gcctctattg aagaaggcgt gccgcgcttc ctgaaaggtc agggccaggg
    22261 ctggatcacc gcagagtacg gcatgctgcc acgttctacc cacacccgta acgctcgtga
    22321 agcggcgaaa ggtaagcagg gtggacgcac aatggaaatc cagcgtctga tcgcccgtgc
    22381 tcttcgcgcg gcagtagatt tgaaagcgct gggtgagttc accattacgc tggactgcga
    22441 cgtgcttcag gctgatggtg gcacgcgtac cgcgtcgatt acgggtgcct gcgtggcgct
    22501 ggcagatgcg ctacagaagc tggtggaaaa cggcaagctg aaaaccaacc cgatgaaagg
    22561 gatggtagcc gcagtttctg tcggtattgt aaacggtgaa gcaatttgcg atctggaata
    22621 cgttgaagac tcagccgcag agaccgacat gaacgtagtg atgaccgaag acgggcgcat
    22681 cattgaagtg caggggacgg cagaaggcga gccgttcacc catgaagagc tactcacctt
    22741 gttggcgctg gcccgagggg gaatcgaatc cattgtagcg acgcagaagg cggcgctggc
    22801 aaactgattt ttaaggcgac tgatgagtcg cctttttttt gtctgtagaa aagtaagatg
    22861 aggagcgaag gcatgaaacc atatcagcgc cagtttattg aatttgcgct tagcaagcag
    22921 gtgttaaagt ttggcgagtt tacgctgaaa tccgggcgca aaagccccta tttcttcaac
    22981 gccgggctgt ttaataccgg gcgcgacctg gcactgttag gccgttttta cgctgaagca
    23041 ttggtggatt ctggcattga gttcgatctg ctgtttggcc ctgcttacaa agggatcccg
    23101 attgcgacca ctaccgccgt ggcgctggcg gagcatcatg accttgacct gccgtactgc
    23161 tttaaccgca aagaggcaaa agaccacggt gaaggcggca atctggttgg tagcgcgtta
    23221 caaggacgcg taatgctggt agatgatgtg atcaccgccg gaacggcgat tcgcgaatcg
    23281 atggagatta ttcaggctaa tggcgcgacg cttgctggcg tattgatttc gcttgatcgt
    23341 caggaacggg ggcgcggcga gatttcggcg attcaggaag ttgagcgtga ttacaactgc
    23401 aaagtgatct ctatcatcac cctgaaagat ctgattgcct atctggaaga gaagccggaa
    23461 atggcggaac atctggcggc ggttaaggcc tatcgcgaag agtttggcgt ttaaagaaac
    23521 tcgccggatg aaaagtcatc cggcgtcata ttactgcaac tgcgcggcaa ttagcggcca
    23581 gcgggcgtca aaatcatccg tcgggcggta tttaaattcg ctgcggacaa aacgtgacag
    23641 cataccttca cagaaggcca ggatctggct tgccagcagg gtttcatcgg tggtgtaacc
    23701 ttcaccctca cgcattctct tttcacgcaa tacctggcgc agctgcgctt caatacgctc
    23761 gaacagctgg ttgatacgcc cttgcaggcg atcctgttca aacatcagcg catgaccagt
    23821 gaggatgcgg gtcaggccag gattacgctc accaaaaccg agaagcagca acacaatcag
    23881 acgcaggcgc gctgtggtgt ctttctcatc tttcagaatc aggttgatgc gagtaatcag
    23941 gctatcttcg ataaactcaa tcaggctatc gaacatgcgg gtcttactgg ggaagtggcg
    24001 atacagtgcc gcttcggaaa cgccgacaga ggcggccagt tttgccgtcg tgatacgttg
    24061 gcttccatcg ctggattcca gcatcagcgc cagagactga agtatttcct cgcgacggtt
    24121 ccttttcgca gtttgttttt ctgccatgtt acaaaatacc cctgaaaata agcacttgcc
    24181 aggcggcacc cacgctatga ccgcaaacga aatgtttgcg gctatgttat gacgttattc
    24241 ggatgcgtat gtgttactga cgaccagagt gaccaaagcc gccttcaccg cggtcggtgg
    24301 cgtcgaaatc ttccaccaga ttaaattcag cctgtactac cggaacaaaa atcatctggg
    24361 cgatgcgttc gccaggttga atggtgaagc tgtcctgacc acggttccac acggaaatca
    24421 tcaactggcc ctgatagtca gaatcgatca atcctaccag gttaccaagc acgataccgt
    24481 gcttatgtcc caatccggag cgcggcagca tcattgccgc cagtgaagga tcggcaatat
    24541 gaatcgccag cccggtcgga accagcgtag tgtcacccgg agccagttct acggcgtcgt
    24601 cgagacaggc acgcaggtca agtccggcag agccagaggt ggcataagtc gggagcggaa
    24661 attccttccc aacgcgcggg tccagaatct taacgtcgat ttttttcatc ataacgggtc
    24721 acgatctcgt cgagtaataa ttggccaagg agctctttgc gctcaagcgg taagacttta
    24781 tctccgtcct gccagaaaag gtgtaatgcg ttgttgtcgc tgttaaatcc ttgagttggc
    24841 tgggaaacat cgttcgcgca gatcagatca aggtttttac ggatacgttt ttgccgggcg
    24901 tattcttcca cattatttgt ttcggcggca aatccaacga cgtagggtcg atggtctttt
    24961 agtgcggcaa cgcctgcgac gatatcgggg tttttaacca tttttattgt taattcatca
    25021 ccctgcgtgg cctgcttttt gattttctct ggggccacgg tagctgcgcg ataatccgcc
    25081 acggcggcgc agccgataaa aatattttgc tgctgtacag aagcattcac ggcggcttcc
    25141 atttccagcg cggtcatcac atcaacacgt ttaacaaacg gtggcgtcgg tagtgaaacc
    25201 ggacctgata ccagcgtgac gttcgcgcca cgacgggcag cggcggcggc gatagcaaaa
    25261 cccatcttgc cggagctgtg attagagata taacgcacgg gatcgagcgg ttcacgcgtc
    25321 gggccggcgg taatcataat gttcagatgt ttcaggtcgt tgacgggcga aaaatgcgct
    25381 accgccatat ccacaatggt taacggatcg agcattcgcc caggaccgat atcaccacaa
    25441 gcctgactgc cactgtctgg cccccagatg agcaaaccac gggaagcaag cacctctaaa
    25501 ttatgctgcg tggcagcggc acggtacatc tgctggttca tggcggggag cacggctaca
    25561 ggcgcaggtg tagccagaca aatcgtcgat accaggtcat tcgccattcc ggcagcaaca
    25621 cgggcaatca aatctgccgt ggcaggggcg agaatcacta aatcagccca tttacccagc
    25681 tcaatatggc ccatagcggc ttcggctgcc gggtccagca gactgtcgga aacgggataa
    25741 ccagaaaccg cctgcaagct aagtggggtg ataaaggctt ttgccgcttc ggtcatggct
    25801 acgcggacgt cggccccgcg atcgcgcaaa cgacgcacca gttcaggggt tttataggca
    25861 gcaataccgc cgctaacgcc gagaacgatt tttttaccgg ccaggctcat gatgattttt
    25921 cctgttgggt gacaccagaa gttggcgatt ttatcacaat acttttgttg tcgtgccttc
    25981 acctgagaat cactttgcga ggcgctttcc aggattgaaa actggccgtc gatttaacgg
    26041 aacggctatg acaggatgcg agcaccacaa aggaggtgaa ggtgaaaaac aattcacagc
    26101 tgttgatgcc gcgcgaaaaa atgctgaagt ttggtattag cgccttaacg gatgtcgagc
    26161 tgctggcgct atttctgcgt accggaacgc gcggtaaaga tgtattaacc ctggcaaaag
    26221 agatgctgga gaatttcggc tctctttatg gcttgttaac ctctgaatat gaacaattta
    26281 gtggcgttca tggaattggc gtggcgaaat ttgcccagtt aaaggggatt gctgaactgg
    26341 cgcggcgtta ctacaacgtg cggatgcgtg aagaaagccc tttactcagc ccggagatga
    26401 cgagggaatt tttacaaagc cagctcacgg gtgaggagcg ggagatcttt atggtgatct
    26461 ttctcgactc ccaacactgg gttataacgc atagccgtct tttttccggc acgctaaacc
    26521 atgttgaagt ccatcctcgg gaaattatcc gcgaagcgat aaaaataaac gcctcggcgc
    26581 tgatccttgc acataatcac ccttcgggtt gtgctgaacc cagtaaagcg gataaactca
    26641 ttactgaacg gataataaag agttgtcagt tcatggattt acgcgtgctc gaccatatcg
    26701 tgattgggcg tggagagtat gtttcttttg ccgaacgcgg ctggatttaa cccgctatgc
    26761 gcgatccttc gggatctttg tctgttcggg acttgagcac atcgctgagt cagcgtatac
    26821 tacgccacct ttgagaatct cgggtttggc atttgggcct ggcaatcgag agttcacata
    26881 gaactgcgat gaccgggctg taaagcctga cgaggcgcca ataccccata cgaagctcga
    26941 gctaatttga tttttggaga atagacatgt cccgagtctg ccaagttact ggcaagcgtc
    27001 cggtgaccgg taacaaccgt tcccacgcac tgaacgcgac taaacgccgt ttcctgccga
    27061 acctgcactc tcaccgtttc tgggttgaga gcgagaagcg ttttgtcacc ctgcgcgtat
    27121 ctgctaaagg tatgcgtgta atcgataaaa aaggcatcga tacagttctg gctgaactgc
    27181 gtgcccgtgg cgaaaagtac taagtactta gaggaaataa atcatggcta aaggtattcg
    27241 tgagaaaatc aagctggttt cttctgctgg tactggtcac ttctatacca ctacgaagaa
    27301 caaacgtact aagccggaaa aactggaact gaaaaaattc gatccagttg ttcgccagca
    27361 cgtgatctac aaagaagcga aaatcaaata attctcgctt tgatgtaaca aaaaacctcg
    27421 ctccggcggg gttttttgtt atctgcttgc ccccatattg actgcatctg ttcattcctg
    27481 gagatgctat gcctgaatta cccgaagttg aaaccagccg ccgcggcata gaaccgcatc
    27541 tcgttggtgc aaccattctt catgcggtgg tgcgcaacgg acgcttgcgc tggccggttt
    27601 cagaagagat ctaccgttta agcgaccaac cagtgcttag cgtgcagcgg cgggctaaat
    27661 atctgctgct ggagctgcct gagggctgga ttatcattca tttggggatg tctggcagcc
    27721 tgcgcatcct tccagaagaa cttccccctg aaaagcatga ccatgtggat ttggtgatga
    27781 gcaacggcaa agtgctgcgc tacaccgatc cgcgccgctt tggtgcctgg ctgtggacca
    27841 aagagctgga agggcacaac gtgctggctc atcttggacc ggagccgctt agcgacgatt
    27901 tcaatggtga gtatctgcat cagaagtgcg cgaagaaaaa aacggcgatt aaaccgtggc
    27961 tgatggataa caagctggtg gtaggggtag ggaatatcta tgccagcgaa tcactgtttg
    28021 cggcggggat ccatccggat cggctggcgt catcactgtc gctggcagag tgtgaattgt
    28081 tagctcgggt gattaaagcg gtgttgctgc gttcgattga gcagggtggt acaacgctga
    28141 aagattttct gcaaagtgat ggtaaaccgg gctatttcgc acaggaattg caggtttacg
    28201 ggcgaaaagg cgagccgtgt cgggtgtgcg gtacgccgat tgtggcgact aaacatgcgc
    28261 agcgggcaac gttttattgt cggcagtgcc agaagtaatt catgcgcgcc ggatggcata
    28321 ccatccggca taaacgctac gctaacttcg ccatcagcgc ctgatggaca ttctccggca
    28381 ggaaatgggt gacatcgccc tgatggcgcg ccacctcttt caccaacgat gaagagataa
    28441 acgaccactc tttcgacggc atcagaaaca cactttccag ttccggcatt aagtggcgat
    28501 tcatatgcgc cagctgcatt tcatattcaa aatccgccac cgcacgcagg ccacgaatca
    28561 gcaccgtagc gtgttgatta cgggcgaagt tcgccatcaa atcactaaac ccgaccactt
    28621 ccacgttccc cagatgcgcg gttgcctgct gtgccagtgc cacacgctct tccagggtaa
    28681 acatcggttt tttaatgggg ctggcggcaa tcgccagaat aacgtgatcg aacatctgcg
    28741 tggcgcgcgt cacgatatcg atatgaccat tggtaatggg atcgaaagta cccggataaa
    28801 tcgcccgttt ttgcataaca acctcaatgc gttttcggtg gcaggtaagg ttccagcagt
    28861 tgaagcagac gctgtagcgc gccctggttt tgatacagta cttcaacggc atgacggcca
    28921 tagaaactac ggtaatcggc gtcggtgagt aaagaggaca cctcttttgc aagcgtagtg
    28981 gcatcggtaa cggtaatcag cccgcttgcc tgctccagcc gcgcgcaaat gtctttaaag
    29041 ttaaaagtat gcggccccat caataccgga atagcgtgtg cggcagcttc cagcggatta
    29101 tgcccaccac gttcaaccag tgaaccgcca acaaaggcga gatcggcaat gccgtacagt
    29161 aacatcaact cgcccatcgt atcgccaact acaacctgcg tgctggtgga ggggacttcc
    29221 cctgaagagc gtgtgatata gcttagtcca gcctggcgga caagattaat cgcatccggg
    29281 aagcgttctg gatgacgggg taccaggatg agcaataaat tcgggaattg ctgtaacaat
    29341 gcctgatgtg cggcgatcac cacactctct tcgccttcgt gagtgctggt ggcaatccat
    29401 accgggcggt gtggtgccca ctggcggcgc agcgtcacgg ctttagcagc caactgcggc
    29461 gttacagaaa tatcgaattt caggctaccg gtaacggtca cctgattatt ttttgcgccc
    29521 agcgccacaa aacgtgcacc atcttcttca ttttgcgcag caatcagcgt aatacgacgc
    29581 agcaagcgac ggacgaattt acccagtttg gcataacctg cggccgagcg ggcagagagt
    29641 cgcgcgttag cgatcaccag cggaatttta cgtttgtgta gcgccgcaat caggttaggc
    29701 catagttcgg tttccataat caacaccagt ttggggtcga ctttattcag gaaacggttg
    29761 agcgcatcgg gcaggtcata cggcagataa acgtgctgaa catccttccc gaaagccgat
    29821 tgtacgcgct ccgaaccggt tggcgtcatg gttgtaacgg taatcggtaa atcaggataa
    29881 cgatggcgca gcgcgcgcac caacgggatc gccgccagag tttcaccgac ggagacggag
    29941 tgcagcataa tgccgcctgg ttttagcgga tggcggtaaa aaccgtaacg ttcaccccag
    30001 cgttttcgat aggccggagc cttacgtccg cgcacccaga gccgtatcca gatcagcggc
    30061 tgaataaggt agagaagggc ggtgtaaagc aattcgagca tagtaaatag ctgacttatg
    30121 gatgtgctgg ggattctatg tatttagctg tggctttacc attacttttc ccgtttttga
    30181 cttaaatagc aatttacccg ccaattcttg ctgaacatct atactttttt tgtattttta
    30241 tgcgattcat tgatttctgc tgtacgtgca ggaaggaatc gcagagcaac agtgaaagaa
    30301 tatggttgct aatgatttta tttgattgat ttctttattg aaaatcaaca atgtgcttaa
    30361 aaatcattcg ggtcggattg ccgcgaaaga agtgatacac tagcgcgtca atatcagtgc
    30421 ggtatcagta ttcaggtagc tgttgagcct ggggcggtag cgtgcttttt ctgcttcacc
    30481 ttaatcggat aatctcaaca aaaagagtta cttgtggata agccatttcg aagaattttg
    30541 ctcattaaga tgcgttttca tggggatatg ttattaacta ctcccgtcat tagttcgctg
    30601 aaaaaaaatt accctgacgc aaaaatcgat gtgctgcttt atcaggacac catcccgatc
    30661 ctgtctgaaa atccagagat taacgcgctc tacggcataa aaaataaaaa agcaaaagcc
    30721 tcagaaaaaa ttgccaactt ttttcatctc atcaaggtat tacgtgccaa taagtatgac
    30781 cttatcgtca atcttaccga tcaatggatg gttgctatac tggttcgctt attaaatgcc
    30841 cgtgtgaaaa tttcccagga ttatcatcat cggcagtctg ctttttggcg taaaagtttc
    30901 acccatttgg tgccgttgca gggtggaaat gtggtggaaa gtaacttatc cgtgctgaca
    30961 ccattgggac ttgattcgtt ggtgaagcag acaaccatga gttacccgcc tgcaagctgg
    31021 aaacgtatgc gtcgcgaact tgatcacgct ggtgttggac aaaattatgt ggttatccaa
    31081 cctacggcgc ggcaaatctt caaatgctgg gacaacgcca agttttccgc tgtgattgat
    31141 gccttacatg ctcgtggtta tgaagtcgtt ctgacgtccg gcccagataa agacgatctg
    31201 gcctgcgtca atgaaattgc gcagggatgc cagacgccac cagtaacggc gctggctgga
    31261 aaggtgacct tcccggaact tggtgcgtta atcgatcatg cgcagctgtt tattggcgtt
    31321 gattccgcac cggcgcatat tgccgctgca gttaatacgc cgctgatatc gctgtttggt
    31381 gcgacagatc atattttctg gcgtccctgg tcaaataaca tgattcaatt ctgggcggga
    31441 gattaccggg aaatgccaac gcgcgatcag cgtgaccgaa atgagatgta tctttcggtt
    31501 attccggcgg cagatgtcat tgctgctgtc gataaattac tgccctcctc cacgacaggt
    31561 acgtcgttat gatcgttgct ttttgtttat ataaatattt tccctttggc ggtttgcagc
    31621 gtgattttat gcgtattgct cagacagtcg ccgcccgagg tcatcatgtt cgggtttata
    31681 cccagtcgtg ggaaggcgaa tgccctgatg tatttgaact gatcaaagtg ccggttaaat
    31741 cgcataccaa tcacgggcgc aatgcggagt attttgcctg ggtgcaaaaa catttacgcg
    31801 aacatcccgt cgataaagtc gttggattca acaaaatgcc ggggctggac gtttattatg
    31861 ccgctgatgt ttgttatgcc gagaaagtag cgcaggaaaa aggctttttc tatcgcctga
    31921 cgtcacgtta tcgccattat gccgcctttg agcgggcaac cttcgaacag ggcaagccga
    31981 cacagctgct gatgctgaca gataagcaaa tcgccgattt ccagaaacat tatcagactg
    32041 aagcggagcg ttttcatatt ctgccaccgg ggatttatcc tgatcgtaaa tatagccagc
    32101 agccagccaa tagccgtgaa atcttccgta agaagaatgg aataaccgaa caacaatatt
    32161 tattgttgca ggtcggttca gacttcacgc gtaaaggtgt cgatcgttcc attgaagcac
    32221 ttgcttcgtt accggattcg ctgcgccaca acacattgct atatgttgtt gggcaggata
    32281 aaccgcgaaa atttgaggcg ctggcagaaa aacgcggcgt gcgcagtaat gttcactttt
    32341 tctcggggcg caatgatgtc tcggaattaa tggcggcggc ggatttatta ctgcatcctg
    32401 cctaccagga agcggcggga attgtgctgc tggaagcgat tactgcagga ttaccggtac
    32461 taacaacagc cgtttgtggc tatgcgcatt atattgtcga cgctaattgc ggcgaggcta
    32521 ttgctgagcc attccgccag gaaacattga atgagatttt acgcaaagcg ttaacgcaat
    32581 cttcattgcg ccaggcctgg gcggaaaatg cgcgacatta tgctgataca caagatttat
    32641 acagtctgcc agagaaagcg gcggatatca taacgggtgg tctggatggt tgaacttaaa
    32701 gagccgtttg ccacgttatg gcgcggcaaa gatccttttg aggaagttaa aaccttgcag
    32761 ggtgaggtat ttcgtgaact ggaaactcgc cgtactctgc gctttgaaat ggcgggcaaa
    32821 agctattttc tcaaatggca tcgcggcacg accctgaaag agataatcaa aaatttactc
    32881 tcattgcgga tgccagtatt aggcgctgac cgcgaatgga atgcgattca tcgactgcgg
    32941 gatgtcggcg ttgatactat gtatggggtg gcatttggcg aaaaaggcat gaatccgctg
    33001 accagaactt catttattat taccgaagat ctgacaccaa ccataagtct tgaagattac
    33061 agtgctgact gggcgactaa ccctccagat gttcgcgtaa agcgtatgct tattaagcgt
    33121 gtcgcgacga tggtgcgcga tatgcatgct gcgggcatta accaccgtga ctgttatatc
    33181 tgtcatttcc tgctgcactt gcctttttcc ggtaaggaag aggagttaaa aatttcggta
    33241 attgacctgc accgggcgca gcttcgcacg cgcgttccac gtcgttggcg ggataaagat
    33301 cttattgggc tttatttttc ttcgatgaat atcggcctga ctcagcggga tatctggcgg
    33361 tttatgaaag tgtattttgc cgccccgctt aaagacattc tcaagcagga acaaggactg
    33421 ctgtcgcaag cagaagcaaa agccacaaaa atcagggaaa gaacgattcg aaaatcgttg
    33481 taacccgtaa cttatttttg ccaaaatttt ggatacagaa taaatatgtc t
//
LOCUS       DAZGVQ010000037        33082 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000037 DAZGVQ010000000
VERSION     DAZGVQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.037"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(367..657)
                     /locus_tag="ACHLAG_001737"
     CDS             complement(367..657)
                     /locus_tag="ACHLAG_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0654984.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTLQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(658..969)
                     /locus_tag="ACHLAG_001738"
     CDS             complement(658..969)
                     /locus_tag="ACHLAG_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0654985.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            1198..2106
                     /locus_tag="ACHLAG_001739"
     CDS             1198..2106
                     /locus_tag="ACHLAG_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0654986.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(2170..3111)
                     /gene="fabY"
                     /locus_tag="ACHLAG_001740"
                     /gene_synonym="yiiD"
     CDS             complement(2170..3111)
                     /gene="fabY"
                     /locus_tag="ACHLAG_001740"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFP0654987.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(3156..3593)
                     /gene="dtd"
                     /locus_tag="ACHLAG_001741"
     CDS             complement(3156..3593)
                     /gene="dtd"
                     /locus_tag="ACHLAG_001741"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFP0654988.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(3590..4462)
                     /gene="yihY"
                     /locus_tag="ACHLAG_001742"
     CDS             complement(3590..4462)
                     /gene="yihY"
                     /locus_tag="ACHLAG_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFP0654989.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(4456..5055)
                     /gene="yihX"
                     /locus_tag="ACHLAG_001743"
     CDS             complement(4456..5055)
                     /gene="yihX"
                     /locus_tag="ACHLAG_001743"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFP0654990.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(5196..6083)
                     /locus_tag="ACHLAG_001744"
     CDS             complement(5196..6083)
                     /locus_tag="ACHLAG_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFP0654991.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(6073..7422)
                     /locus_tag="ACHLAG_001745"
     CDS             complement(6073..7422)
                     /locus_tag="ACHLAG_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0654992.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLILMALRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(7521..8954)
                     /locus_tag="ACHLAG_001746"
     CDS             complement(7521..8954)
                     /locus_tag="ACHLAG_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0654993.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASSTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(9024..10034)
                     /locus_tag="ACHLAG_001747"
     CDS             complement(9024..10034)
                     /locus_tag="ACHLAG_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFP0654994.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(10067..10954)
                     /locus_tag="ACHLAG_001748"
     CDS             complement(10067..10954)
                     /locus_tag="ACHLAG_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFP0654995.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(10979..11857)
                     /gene="yihU"
                     /locus_tag="ACHLAG_001749"
     CDS             complement(10979..11857)
                     /gene="yihU"
                     /locus_tag="ACHLAG_001749"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFP0654996.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPCKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            12030..12926
                     /locus_tag="ACHLAG_001750"
     CDS             12030..12926
                     /locus_tag="ACHLAG_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP0654997.1"
                     /translation="MTKIACVGITVQDRIYSLLTLPDGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            12966..13754
                     /locus_tag="ACHLAG_001751"
     CDS             12966..13754
                     /locus_tag="ACHLAG_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP0654998.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(13865..15130)
                     /gene="yihN"
                     /locus_tag="ACHLAG_001752"
     CDS             complement(13865..15130)
                     /gene="yihN"
                     /locus_tag="ACHLAG_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0654999.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(15232..16212)
                     /gene="yihM"
                     /locus_tag="ACHLAG_001753"
     CDS             complement(15232..16212)
                     /gene="yihM"
                     /locus_tag="ACHLAG_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFP0655000.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(16220..16930)
                     /gene="yihL"
                     /locus_tag="ACHLAG_001754"
     CDS             complement(16220..16930)
                     /gene="yihL"
                     /locus_tag="ACHLAG_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0655001.1"
                     /translation="MTGNQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(17147..18970)
                     /gene="typA"
                     /locus_tag="ACHLAG_001755"
     CDS             complement(17147..18970)
                     /gene="typA"
                     /locus_tag="ACHLAG_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFP0655002.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            19343..20752
                     /gene="glnA"
                     /locus_tag="ACHLAG_001756"
     CDS             19343..20752
                     /gene="glnA"
                     /locus_tag="ACHLAG_001756"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFP0655003.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            20925..21974
                     /gene="glnL"
                     /locus_tag="ACHLAG_001757"
     CDS             20925..21974
                     /gene="glnL"
                     /locus_tag="ACHLAG_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFP0655004.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            21986..23395
                     /gene="glnG"
                     /locus_tag="ACHLAG_001758"
     CDS             21986..23395
                     /gene="glnG"
                     /locus_tag="ACHLAG_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFP0655005.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            23507..23617
                     /gene="yshB"
                     /locus_tag="ACHLAG_001759"
     CDS             23507..23617
                     /gene="yshB"
                     /locus_tag="ACHLAG_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFP0655006.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(23807..25180)
                     /gene="hemN"
                     /locus_tag="ACHLAG_001760"
     CDS             complement(23807..25180)
                     /gene="hemN"
                     /locus_tag="ACHLAG_001760"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFP0655007.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(25369..25878)
                     /gene="yihI"
                     /locus_tag="ACHLAG_001761"
     CDS             complement(25369..25878)
                     /gene="yihI"
                     /locus_tag="ACHLAG_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFP0655008.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(25997..26163)
                     /locus_tag="ACHLAG_001762"
                     /pseudo
     CDS             complement(25997..26163)
                     /locus_tag="ACHLAG_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26460..27092
                     /gene="yihA"
                     /locus_tag="ACHLAG_001763"
     CDS             26460..27092
                     /gene="yihA"
                     /locus_tag="ACHLAG_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFP0655009.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(27474..30260)
                     /gene="polA"
                     /locus_tag="ACHLAG_001764"
     CDS             complement(27474..30260)
                     /gene="polA"
                     /locus_tag="ACHLAG_001764"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFP0655010.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            30624..31556
                     /gene="yihG"
                     /locus_tag="ACHLAG_001765"
     CDS             30624..31556
                     /gene="yihG"
                     /locus_tag="ACHLAG_001765"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFP0655011.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWSLDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(31597..33027)
                     /gene="yihF"
                     /locus_tag="ACHLAG_001766"
     CDS             complement(31597..33027)
                     /gene="yihF"
                     /locus_tag="ACHLAG_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFP0655012.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         8334 a         8500 c         7903 g         8345 t
ORIGIN      
        1 tatttgcata atctgaatgg tgtagccgaa aaacgtcagc atcctaacag caccaggatt
       61 taaggtgaaa ttaatcatcc atcacagacc ggtcagtact gccaccaacc ggtctttact
      121 tgttcatcgt tataattttg ttggtaacag ttcgggaaac aaggaattcc ttccggaacc
      181 ccccggtatc atccgatagc attgacgtca atcatcagcg cgacgatgag tttttttaat
      241 cgcatcatag ggtggcgacg ttcagcacga ttacgggtca tagcaaaact cctgggcagg
      301 cgaccactat gccgctttca caggagttct gaatgctagt gctttctgta tggcataacc
      361 atgcctctaa cggttaagtg cttgcagcac atgttgcgga tcattggcaa tcgctcgcag
      421 caaagccttt gcaggcccgg tcggtgaacg tcgcccctgc tcccagttac gcaacgtgtc
      481 tacgcttaca gaaataagat cggcaaattt agattgtgac aaccctgaag cgcggcgtat
      541 ttctttgatt ttcatagcat caacttcaaa agtccgcgaa ggctgtagcg ttccttcaat
      601 aatttcattc atctgggcca tgctgtgagt caaccgctca aaaagtgcct tatccattta
      661 ccacctcgta tttaacaacc ggagcattgc tttttcctgg ggagacagat catctttaat
      721 acccttacga taaataagca gtaatctgat ctcataatgt ttaacctgat gaaagtaaat
      781 aaccctgaca ccagcacgtt tcccctttcc tcctgaaacc catcgaacct ttcttaaccc
      841 accagtttca ggtattacct caccatagtc aggattcaag gccaggaaaa actgaaaacg
      901 actgaattca tcgtcggtca gcagtttttg aacatcttca gtaaaaattt ccgtttcgat
      961 gaagagcata attatacgcc attggcttag agtaacaatc ctttttgtac gtcattgacg
     1021 tacccttgaa accactagct tactgatagt gctccagcaa aaccacgtct ctgccgggtt
     1081 tgtctttaga tttgcgaggc gctttccaga tttttcacgc ccgtgaattt ccctgtatcc
     1141 atccagcaaa aacgggtgta taagactgta cattcataca gcaaaataag ttctactatg
     1201 gcactggaaa aaggaattgc agagttagtc gaaaagttta tcgctgcggg tcgcccctca
     1261 tcgcgtcaac aaagtattgc gcagcgcagg gagggctata tcgccagcgc tgtccttgcg
     1321 ggtgagacag aaacgcgggt agatattcag actatcgaac ttgaagggat gaccttacga
     1381 atagtgtcgc cgctgaatgc cccgacatta ctcccgacga taatttatta ctacggtggc
     1441 tgttttgtta gcggcgggtt cgcaactcac gacaaccagc tgcgccagtt agcctattac
     1501 gggcaatgtc gggttattgc cgtccagtat cggctggcac cggaacatac tttccccgcc
     1561 gcccatgatg atgcgcaacg aggtgcagaa atagtctggc aacacgccga acgactcggc
     1621 gttgataaac aacgtataac tcttgccggt gacagtgcgg gcggacatct ggcgctggta
     1681 actgctttgc gattaaagcg tgctggcgag tggcaaccgg cacaactcat cttaatttat
     1741 cctatgcttg atgcgactgc gcatttcgaa agctacatac gcaatggtca cgattacatc
     1801 atcacacgcg acactttact gagcggcttt gagatgtatc tgccaggcat tgaacgcagg
     1861 catcctgaag ccagccctct ttggcgcgat gatttcaatg gcttgccacc tgtacatatc
     1921 attaccgctg aatacgaccc actttgcgat gaaggtgaag cgttgtatca ccgcatgaca
     1981 gggcaaggag tgcaatgcac ctgccaacgc taccttggcg ttatccacgg attttttcag
     2041 ttaggtggta tcagcgaggc cgcgcgatcg gcactacggg atgttgcctg gagagcggga
     2101 aggtaacttc ggacctgggc cctgaaaagg ttttcagatt gaaaaccttt tcagtcaagt
     2161 aaaacgcccc tactcttctt cgttcccgcc ctcttcatac gggccaaatg gcttcgcggg
     2221 cagaacgata tacgtgcctt caaacactgc acccggcgtc tcgtcgccaa agatttcaac
     2281 ttgcatctgc acccgtgctt ttcgtccgcg cgccagacgg tcgagatcgc cgcttaaggc
     2341 accaaggtcg gctaccgcat gaggtttacc gctaatcggc ttgctgtagc ggatatgcgc
     2401 atccgccaga ataatcgttc cgccgagatg gcgttcgcgc agcatcagcc agataagccc
     2461 ccaaccggtg agcgtcgcca gcgagaataa actcccggca aacagcgtat ggtgcggatt
     2521 ctgattgccg gtttccggca tggtggtgat aaatttttgc ccggtatatt gttgaatacg
     2581 cacgcccatt ttttcactaa gcgggatatg ttcgtaccac gcctgttgca gctgcgcgca
     2641 ccagtcgccg cgatgcaaaa tatcatccag agtggcgacg ggcttaatca tcaaaaaatg
     2701 gcgaatcggc gtggtggttg gcgtggtgat ttctccctga ttaacaaacc ccagcttggc
     2761 gaaaaactcc accgcgtctt cacgggcgct acaggtcacg cgcttaacgc cttcctgacg
     2821 cgccaccgac tccagggtca tcgccatcag cgttcctaac cctttgtcct gcacgtccgg
     2881 gtgaacggcc ataaagcgaa tggacgcttc attgtcggca ttaatataca gtcggcctac
     2941 cgccaccaga ttaccctgct cgtcgacgac catctggtga tgcgccatcg catcccacgc
     3001 gtcgcgttcc gaaccttttg gttgatgcag gggcttacgc aacatttccc agcgaaactg
     3061 atagtaacgc tctaattctt cttctgtttg tggaacccga aggtgataca tagcgatact
     3121 ctctcttgtt tcccgtgaca accctggaag ctggctcata cctgcaacca gaatgtcacg
     3181 gggccatcat tgaccagcga tacctgcata tcggcagcga agcgtcctgt ttgcgtgttc
     3241 atctcttgct ggcggcagcg ttcgacgaaa tagtcatata acgcctctgc gcgatccggt
     3301 gatgcgcctt tggagaaact tgggcgcatc cctcgttcgg tatctgcggc gagggtaaac
     3361 tgggaaacca ccagtacact gccgcccgcc tgttgcacgt tgagattcat cttgccttcg
     3421 gcatcgctaa agatgcggta gccgagcaca cgctcgcaca gacggtttgc tttctgttcg
     3481 tcgtcatcct tttcgacacc caataacacc aaaagtcccg cgccaatttc gcccgtcact
     3541 tctccctcca cggtgacgct ggcacgggtt acgcgttgaa ttaatgcaat catggttcgt
     3601 cgtcttcttc ttgttcagct gcttgtttta gtttgcggta ttccccgaga gtgacagtaa
     3661 tttccgcgcc aagcaagacg atacaccatg tccagtagac ccaaacaaag agaatgggga
     3721 tcaccgccag cacgccgtaa atgagctgat atgacgggaa catggtgata taaagcgcga
     3781 aacctttctt tcctgcttcg aacaggagtg cagcgacaaa cgcgccgaca atcgcgtcgc
     3841 ggttaggtac gcggatggta ggaacaatgc tgtacagcaa ccagaaggag atccacgaca
     3901 acagcagcgg aaaaatacgc agcacgttgt cgatgacagt attgagatcg ctcgcccagc
     3961 gcagggagag caaataggaa ctgatcgcca gactggcccc tgccagcagc ggccccagcg
     4021 ttaaaatcat ccagtacacg gcaaacgagt aaatttttgg tcgcgctcgt ttactgcgcc
     4081 agatggtatt caacgcgcta tcgatggagt acatcaacaa taatgcggtg acgatcagcc
     4141 cgcacgcgcc aacggcggtc atcttgttgg aattggcaac aaattgttcg atataccgct
     4201 gaataacatc gccagtagca ggcagaaagt tggcaaaaat aaagtgacgc aactgaatgc
     4261 tgacgtcgga aaacatggga aaagcggcga aaagcgcaaa aacaacggca accagcggca
     4321 ctaatgagag caaagacaca taggcaaggt tacctgccag ggttgtcatg ttgtcctcat
     4381 caatacgttg ccagagtagt tttagccagg cccatagtgg acgagtacgg tgcctggctt
     4441 tgtcctgaat ggtttttagc ataacacctt cgcgaaatag tccgggatgg tggttttatc
     4501 tttcaccaga atactggtaa tgcccagctg attggctcct tctatattat cggcgttatc
     4561 gtcgaaaaag accgtatcgc tgggtgaaaa accttccgcc tgcaaaacat gctggtaaat
     4621 tcgtgcttca ggtttgcgca tccccagatc ttgcgacaga tagatatggt cagcagcatc
     4681 acgaatttcc gggtattctt ccggccagaa ggtggtatgc aggcggttgg tattggaaag
     4741 caccaccacg cgatgcccct gctcacgcag tttatgcatg atggcgatca cttccgggcg
     4801 cagcgcaaca aacaccgcct gccagccgtg agagaactgc tcgtagctta gcggtagagc
     4861 catctcatga cacagcgcct ctgcgaacgc ttcgtcgcta atttccccac gctcatgctg
     4921 atgaaacgcc tcccccatat gaaaactctt cttaagcgat gccagcggaa tacgcgttaa
     4981 atcgctccag gctcccagca cacggttaaa gtcgatatcg acaatcacat tacctaaatc
     5041 aaagatatag agcatttttg cctcctttcg cgccacaaga aattaactgt agcgggaaag
     5101 gatgggtttg gctatgtggc aataagggta atcttgtgat gctgattgcg aaaaagcaga
     5161 gtaatttcac tggctttttc gcaatagatg aatggttaag attcaggtgt aaattgccaa
     5221 cgagtttcga tgaccagcgg cctatctggt gtcaccaatt ccggcgttag atttattgcg
     5281 ttaggtgcct gagtcagcgg gttaatacag gtggcatcgg gctgtttatc aaataccacc
     5341 agactgtttg cagaagagat catggtcatc gcgagtttac caggccacag taacttaacc
     5401 ttcaccccgt cataaaaacc aaaacagtca tcccagggac cggcttgagg agagatacga
     5461 tttccagttg gcagttcatc actacccgct tcttcttgcc agtccgcatc aaaaagcacc
     5521 tgcaacgatt ctgtattttg cggagtcagc tttttcgcaa accacggatg ccagcctgcg
     5581 gaggccggga atgtatcggc atatgaatgg acttccagtt gcaaaaccag cgcatcgttc
     5641 tccagcaaaa aagtctgaat cacttcccct tgccagggcc acggtgaagc caggggcata
     5701 cgtaaagtca gggaatctgc tgttttgtcg atgatttccc acgttgagta acaagccata
     5761 ccatgcaacg catgtggtgg tttattggcg ggtaaagaat aacattgccc accagcattc
     5821 agcgtcgcat tccccagtcg ccctgcccac ggcaccattg ggaaacagcc atactgaaaa
     5881 gctctgcgct gcggctgcca ttggcgtaat acttcagaac caaatgcctt taacgaggtt
     5941 atcctggcac catcatgcgg ataaatctgc aggtgaatat tgcctttttc cagcgtcact
     6001 aactctccta caggcccagg ccatggcgcg tcaaacgcct gttgtagcgc caggtaatgc
     6061 tcgttttttc gttcagtttt catgagattg ttccctgatg tagcggtaag aagcggtttc
     6121 ttgttcatcg gtcggctcaa tcagccagac gcaaacaaag gaaagaactg agacagcaaa
     6181 taaaaacaga gcgacatacc acggttttcc tccccctagc gccagcaaac ttgaggcgat
     6241 aagggggctt agtccgccaa gaatcgcgga aaattgataa gcaaaagaaa ggccggtata
     6301 tcgtactttg gtaccgaaca tgcgggtgaa aagtgttggc tgcacggcaa acatcatcgt
     6361 cggacctaag ttgtagccga ggatcatact gcaccagatg atgagcgtac ttttgctatc
     6421 cagtaataag aagaaaggaa acgcgaacag gacacaaaat cctgctcccg acaaatacag
     6481 acttttttga cctatacgat ctgataacca tccaaccaat ggacaactga aaataccgat
     6541 cgccgaggca atcaacatac ccgtcagggg aatatctcgc gacaatgcta attggctgga
     6601 aatatagaca ataccaaagg cgttaataat attagaggag acactttccg ccagcctcgc
     6661 acccagtgcc aataaaatat ttcgtggatg ttttttgaat aattcaatca acggaggaac
     6721 ggacttttct ttattattcg tcgttttttg cttttgaaaa tccagcgttt cttcggtatg
     6781 caggcggata aacagcccaa cgatcaacat caccgcagaa agccaaaacg gaatgcgcca
     6841 tccccaggag agaaactgct ccgaagtaag aaaatggtta cagagagcaa aaataccggt
     6901 tgccagcata atacccacag acgcagccgt ttgcgggagt gaacctaaaa atccgcgtcg
     6961 ggattcgggc gctgattcta tggtcatcaa tgccgctccg ccatactcgc caccaagacc
     7021 aaagccctga attaaacgca gcgccattaa tatgagcggt gcccagatcc caatttcctg
     7081 atacgtcgga ataaaaccaa tcaaaaatgt ggagcaaccg acaatagcca gtgtccagat
     7141 aagtgttatt tttcgcccaa ttttatcgcc aaaatggcca aagacgatac cacctaaagg
     7201 acgggtcaaa aaaccaacgc caaaggtagc aaaggcggct ataagaccta taaaaggatc
     7261 gctgtctgga taaaacagcg gtgcaaatac cagtcctgct gccgtgccaa aaataaagaa
     7321 gtcataccat tccattaacg cgcctgcgaa actgcccata acgacacgtc tcatggcttt
     7381 accggaaagc ccttcacgcg gtgcgggtaa cttttcagac ataattttcc tctcctggta
     7441 ggcgagaaat ctgggatacg aaccactaaa cgtgggcagt ttgaattaat gcgcatcggt
     7501 ttaagccgat gcgcattaat ttatttcttc aattcagcca gaacctctgc atcctcagca
     7561 tcaacatttg cacccagtgg ttcccaatta atcaatacgc aggtcaggaa ccccaagaat
     7621 ggcacgattg ccaaccagta aaacgcttca gttttcaacg ccccccacag catcggccag
     7681 aaaatgagtc ctgcaattgc ccctgtgcgc ataatagcgc gggcaaatcc gaccccagta
     7741 gggcgaatag cgggaggata agagagtgtg gcaatggtca tgcccagccc accaggaccc
     7801 gctgaatgga agaaaataat tgatcccaat aaccctaaag acagccatgg attagtcgtt
     7861 gccagtgcgc ctaacgacag caatgctaag gtcacaacag caaatccata catcgactgt
     7921 cggcgagtac ccagtcgttg gagaataaac gagccgatca tcccgcctgt caccccacaa
     7981 agggcattca cgacggcaga acccgtcaac gcaccagtaa gcccgccact tattattccc
     8041 gccaacgtaa gaggaagata aacacctacg gcgttatatt gccacgcctg catggttgcc
     8101 acgacacagc caagaatggt gcgtttgcgg taacgatcat taaataagat gccatatcca
     8161 ccactgtatt tattctctgc tttttccgag aatttcgctt ctgttgtact cgatgccgca
     8221 acgcgagcct gaacaccata acgttttccc agaatgtcgc acgcttcctg gtaacgcccg
     8281 acgcgtgcgg cccacattgc ggattcacca ataaagaagt aacgcaaaat catgaagatg
     8341 actgcaaaaa tagctccgat gaatattccg taacgccaca attgttcttc agcgataccc
     8401 gacagaagca gcggcaagag caccaaataa acaacagttg tcgaaacata ccaggccatt
     8461 tgccagaggt tgaccgacgt tccggttttc ttcgacgttg aaccacgaag ctctgccacg
     8521 gcattggttg ccaaagggaa gtcgatgcct aaaccaaagc ccatgatgac gcggcacacc
     8581 agcacccacc agatactggg cgcaatagct accgcagcag ccccgatggt gcacagcccc
     8641 atgccaataa taaatgctcg cttttgccca aagcggttta ttactcggtt cgctaataaa
     8701 ccgccaatta acgcggcgat taatactgac gcattaacgg ttgcggcaag gcctggagaa
     8761 atgccaaatt gagcggcaat gtatttatta ccaaacccca ccatcgccgc ctggtaggca
     8821 tcaatcaata tgccgcccaa agcaataaaa ataacgccga tactggtgtt gattgcagag
     8881 ttcttattaa caatatcaat tacgtcctgt ggccgactaa ttgaataagt tgcaggactg
     8941 gtgatttgac tcatcgcagt gtccttttta attgttgtag ggtggcgctg acgtgacgca
     9001 gcgccagaca gagatttaga aaatcattcc ggcgcaaatt gcagcatgac ctttatggag
     9061 tcattttgct gcgcgcgttc gaatgccgcc gctgcatcct caatggcaaa taaatcggta
     9121 accagttcac tggtatcaaa ccgcccatcc gccagccatt cgataacagc atcgaaatct
     9181 tcagggatgt acatgccaga gttgagcaag tcacgctcga aacgttgcat catcggtaat
     9241 ggtatttcac gcggcccact tggtacgccc atgcacacga cggtgcctcc ggcatataca
     9301 cgggtacatg catcgttaag cgtggcctgc gctgcaacga catcaatcgc ccccgtaaat
     9361 cgcacatctg cggccagctc acctggtgcc caaacttctg ctgcgcccag ctttaacgcc
     9421 agcgcgcgtt tggcagcgtc aggttcaatc acggtaatct tacctgcccc cataatgcgc
     9481 agtgcctgca cgatggataa gcctatcgtt ccggcaccaa tcaccagtac gctttccaga
     9541 gaagctttcg gcattcggtt aacgcagtga cgggcacaag ccgccggttc ggcaaaggcc
     9601 aacactttta gcggtaaaga ggctggtgcg acatggcaat tacgcgcagg aacaatgtgt
     9661 tgtgagcgag caaagcccgg tgcgcgaaaa ccagcaacct gcggtggttc acaaagatta
     9721 aaacgtcctg ctttgcaggc tcggcattcc atgcaagcca tgatgggatc gaccacaaca
     9781 tgatcgcccg gctgtacatt tttaacgtcg ctgccaactt ccgtaacgcg cgctgcaatt
     9841 tcatgaccgg ggacgacagg tggtttagca aacggatgac cgcctttcag aacatgcaga
     9901 tccgaaccgc atatcccata gaacacaggc gcaatccgca cttcgtgcgg tttaagttcc
     9961 tggtgtggta cttcaacata ttgagtggtg actttcccaa tatctgaaaa taaaacctgg
    10021 gtaattttat ccattgttat tctccagaat cgatatccat aggggattag cgttgcgcga
    10081 taccttcatc gacaatttct gccaaccgtt gtaaacgagg aacagagaca tcgcgcagca
    10141 tagtttgtgg atcttgtgca ccaacaaccg acgcccaaac cgctcgtcca gccaggaagc
    10201 ctgacgcacc acctttcatg gcaatactta ctgcacggcc aaaaatatcg gcatcaacgc
    10261 cagaagaaag aattacccac ggcatcttca tctgatcgtt aagttgctga caggcggcaa
    10321 gtaaggtttt ttcatcgcct ttgcccccta atggcatctc ggctttgtaa agatcagcct
    10381 ctgttccacc aagctctgcg gcggcggcaa caatggcgct ttcacgatca aagtcccaac
    10441 cgcgacgagg tggacgcacc acaggttcaa taatgctaac cagcccggca ctacggcagc
    10501 gacgaacaaa tttatcaacc attgccagac gttcctctgc gggttcatct tcacgccaga
    10561 gtacaagtaa tttcattgcc ttagccccca tttcacgggc tttatgcggg tcgacggaca
    10621 tatctatttc aacgctatca accgggatgc cattacctgg gataaaaaga tcggcagcga
    10681 caatcaggcc gcaagaatta gcaacagcgc cctgctcgac tatttgctca agacaaaatt
    10741 gtttatccgc cagaacggca gaagcataag gagaaaggat tcgggtcgcg gcaactttaa
    10801 aatcggtgag tacactgtcg gcgatgggtt ttggctgccc tgctgccgca aacatcaagc
    10861 gcatggcctc acgctgatcg acagctaaca tggcaaatcc accacctggg cgggagatat
    10921 ctttcagcgt aaatggggtg aagtgggatg acatagaaac tcctgttaat agataaaatt
    10981 aaagaccagc ctgctggcga acctgattta gcagggcact ccagtcctgc cgccctttgc
    11041 cgtcttgact tgctgcagcg taataacttt ctgctgcaat ccccatattg agcggtgccc
    11101 ccgtctcccg agcgacgtca actgcaatac gcagatcttt caacgccaga tccaccatga
    11161 aggcgggtga aagatcgcct ttcagcactt tgcaaggcca ggtggtcgta aaatgtcctt
    11221 tccctgctgc cgtaccactc atcaccttaa ttgcgacatc aagatttaat cccagtgact
    11281 cacataacac agcggcctcg gaggacagcg cattaagcgc aatgctcata tagttgttaa
    11341 ttagcttcac gcggatcccc attcctgggc cgccagcctc gaccagctca ttacccatgc
    11401 acatgagaat cgggcgtgca cgtgcaatct gctcatcagt gccacccgct aaaatcagca
    11461 atgtgccatc aatcgcattc acggacgttc tgcccacggg ggcgtccatc atactgatgc
    11521 ctttttcttg taactcccgt attaatgcat cggtctgtaa gggatgaata gtcgacatat
    11581 caatcaataa cgcgtcagat gaaacagcct cacagacgcc tttttccccc aacagaacct
    11641 gtcgaacaat gtcaccattg ggaagcatag tgatgataaa ttcagcgcct gctgccgctt
    11701 ccgctggggt ttgtgccgca gtggccccct gcgtaactag cgcatcgacg gcctgtgcat
    11761 tgacatcaaa tacctgcaat gaatgccctt tttgcaatag attgttagcc attgggctac
    11821 ccatctgccc cagcccgaga aatgcaatgg ttgccatgaa tactctcctg tctgacattg
    11881 acaaaataat gacaattgaa aattcgtttt cgtctgtttt tgagcatatt tgtaatttat
    11941 gttcaaaaac acgacacgca tcactaattt gatcatttgt gtcatttaaa ttgaccgaag
    12001 acgacaaaat taaacaggac aaggcaaata tgaccaaaat tgcatgcgtg ggtatcacag
    12061 ttcaggacag gatttattct ttgctcactc tgccggatgg cggcggaaaa tatcaggcaa
    12121 atcattatct tgagattggt ggcggcccgg cagcaacagc cgctgttgca atcgcaaagc
    12181 ttggcgtaga ggttgatttc atcggtcgcg tcggcgatga cagttgtgga aataccttac
    12241 ttgcagaact tgagggttgg ggcgtcaata ccgcattttg ccgccgatat cctaacgccc
    12301 gttcatcgca atcagcgatc ctcgttgatc agcatggcga acggattatc gttaattatc
    12361 caagtcccga tctcggtact gatgccgaat ggttagaagc gatcgatttc tctcgttatg
    12421 atctgattct ggccgatgtt cgctggcata gcggtactga aaaagcattc tcgctagcgc
    12481 gtcttgcggg tgtgacaacg cttttagacg ccgatatgac accgcaggat atttctccac
    12541 ttgtcgcgct tgccgatcac gccgtgttct caacaccagg gctgaaacgc atgactggcc
    12601 tgcagtcacc tgaagaaggt ttgttccagg caacgacaca aacagcaggt aaagtgtatg
    12661 ttacgctggg ttctgaagga tcgttgtgga ttgaagatgg tcatttgtgt caacaggagg
    12721 cattttctgt caatgtggtt gataccactg gagcaggcga tgtttttcac ggcgcactcg
    12781 cagtagcgtt ggcagaaaaa atgccaacaa aagaggctat tcgttttgcc agtgcggttg
    12841 ccgcgatgaa atgtacccag cctggcggtc gggccggcat tcctaatcgt gagcaaaccg
    12901 aatcattttt gtcactttat gcgtaaaatg gtaaacatct accgaggagg ggcaagggag
    12961 tattcatgag tatcatcgaa gtgacaggta atccacgcca tgaccaactc gttcatctaa
    13021 tcgcagaacg cggttatatg aatattgaag aactggcaca actgctggat gtttcaacgc
    13081 aaaccgtgcg ccgggatatc cgaaaactca gtgagcaggg cttgatcacc cgtcatcatg
    13141 gaggggccgg gcgcgtctcc agcgtcatga ataccgcttt tgagcaacgg gaactttcgc
    13201 tcaccgccga gaaacgggcg atcgctgagg cagtcgccga ttaccttccc gaacgctgta
    13261 ccgtctttat caccatcggt acaaccgtag aagccgttgc cagggcatta ctcaaccggc
    13321 gtgatttacg cattatcact aacagcctac gtgtggcaca gattctttat aagaatcagg
    13381 atattgaagt gatggtgccg ggaggcactt tacgcgctca taacggcggg attatcggcc
    13441 caggagccgt ggattttatt gaaggcttcc gcgcagatta tttaatcacc agtataggtg
    13501 ccattgaaca tgacggcacc ctactggaat ttgatgtaaa tgaagcgtta gtcgcgagaa
    13561 cgatgattaa acatgcgcgg aatacattgt tagtggccga tcatacgaag tttgccgcgt
    13621 ctgctgccgt ttcaattggc aatgcacgga atgtcagggc tttttttaca gatgccccgc
    13681 ctcccaattc tttctgccag ttgttaagtg aagagaatgt tgaactggtg gttgccgagc
    13741 aggaagtttc ctgaaattga aacttcgaaa aatccgctgg ggattcagcg gattttattt
    13801 tgccggatgc ggcctacggt tgatacggtt ttcgttggcc ggattaagca catccggcca
    13861 ttaattaagc ttcagccgcc agcatctctt tcttacgttg gctaatacgc ttaaccagca
    13921 ttacggaaac caccgcacca cagaatgcga agcaggccat aatgccaaac acgattttgt
    13981 agccaataat ccccggattt aaatccagaa tgtagccata cagactgaag cagaacatcg
    14041 ccggagcgta accaataaag ctacccaacg ccatcgccgc gcctgtttta ttttcagcaa
    14101 tttttgcttc gccgataggt gcaaaaaata cggctcgctg tgtaaagact atcgcgccaa
    14161 agcccagcgt acatgccatc cctaaataga ccggcatact ttcgtgcggc agcataatca
    14221 acagtacgag cgcagcggta ctgatgataa aggtgtagca tagatattta ctcggcgatt
    14281 tcaggatctt atctgaaatc atgccaccaa tcggtccacc aatcattttc agacagtatt
    14341 ggttaatgat gccgtaagcc cccaccagcg caacgggcaa tagatagatg tttttcagga
    14401 atggaatgaa gaatgtcagg ccacagtaaa ccgcgtaaac gaagaagacg ttaaaagcga
    14461 taagccagat ggttttgtct ctcagcaccg aggtcatact ggtgttttgc gatgctccgt
    14521 cttctttttt cacctcaacg gacggtgcct cttctttgtc attcaggaca aagaaaataa
    14581 taatgcctac ggcaatcaca ataagggaat agaaccagat gcctgcttta aaacccaata
    14641 agccactgcc aaaccaggta aatactgcca acgcagaaaa tgccaccacg gtatcgacaa
    14701 tgccacgccc tgtttcgaag aagccaaaca accgaccttg ttgttcgcta ttgcccaatc
    14761 gacttaccga cttgagcaag accggccagt tcatcatgtc gcaagtaacg ccaaatagcg
    14821 cccagacaaa gaggatgccc caataccccg gcattgtcgt caaataaaca cccagtaatc
    14881 ctgtcgcaat gagtgagaat gacatggtgt atctgcgcgg tagtttatcg gcaaaataaa
    14941 tagacagaaa aaaacccact gtcgtgacaa atgagtttac cgacatagca ttaccaattt
    15001 gaccattggt caaatggaaa tattcctgca tcgggatata aaacgcatct ttcagcgacg
    15061 gtaatttata aattgtaccg ccacacaatg ttaatagact aaataacgcc catttctttt
    15121 tcgtgagcat aacatcccct aagcgatttt taaaatcact gttttaaagg caataagata
    15181 ccgaccattc atatggaatg gttttattaa ctgcgtatga gttaaaactt attagtggtt
    15241 cagaagatgg tttgcctctt gtttgatttg ttgtagtacg ttacgcacat gatttatctg
    15301 attaattgca tcttcttttt ctttacgtaa ccgcgcatcc agtaaatgat tttctggcaa
    15361 tggtgtttca ctcatctggc gataagggat ccacggatta tcatcttcgt cttcaaagcg
    15421 gaacgccggc gcatagtaat caacctcttc ttccaggcca tatgccgtca cctgcggctc
    15481 atcatcaccc aggcatataa ggtgcgttaa taacgctttg aacggcatat caccctgacc
    15541 tgaaatacag gctttatgac ccaggccacc cggttcttta acgatcaagg catctttgat
    15601 atggacctgg gtaatatgcg gcgccatcgt ttttaaagcg tcgatgggat gctcatttgc
    15661 attgatcatg ttcgcaaaat caaacagtaa ggaaagagat tccatctcac tttctttgac
    15721 cagcgacacc agctcatggc tttttaaatc ttcatgctgt tcgatcgtaa aagtcaggcc
    15781 gctgtcctga tacgtttccc gtacataggc aatgtcgtta gcgatgatcg ataatacgtc
    15841 gcgcaaatta ccttcataac gtggataaaa acgtacggac gatgccccag ttttcaacgc
    15901 aatggcgacg gcttcgtcaa tagatgcctt atctgaggcg ctggtttcaa tatgaatatc
    15961 aaggttcaga cggcgggctt tatctccaaa ggcagagagt tctttatcgt ccatattacc
    16021 aagagaaaaa cgctcgccat ccagaacatg gattttgacg ccacgtagat tattttcact
    16081 tgcgatatca agtaaatcag caggcaacac tctttccagc cgcatattta aatgaaaggc
    16141 ataagcatgt aaataaagag gaagagtgtc gacacgttgt agaatctttc ttgcgttatt
    16201 tattgtcacc ataaaacacc tacagattaa tgatgaggtc tacggcctca aatcgccaat
    16261 attcaatatc tatctggaca atattgcctt gctggtcggc gcgatgcttt tcaattaaga
    16321 tcgctggcat acccgcagaa ccgccaagat acttgctgat atcgcccggc attctgacgg
    16381 gtttgaaaat caattttttt actaccgtct ctttttggta gcacttttcc cagactgcag
    16441 aaaatgagtg gttttcaagt tgttcaataa aacccggagc aacttcagga ttaatatatg
    16501 tttcgtgata gaaaacttta tgtccttcca gcgcccccca gccggtgatg cgatagagtt
    16561 cagttgatgg tgtcactgca atcagtggcg cgagcgtctc gggaatatcg ctggtacggt
    16621 ttttctcggt aaacccccag gaaggctctc gtccttgctc aattgcggcc cgctgaaagc
    16681 tcgccgacaa ctccggactg taattaaatc gcggctgggt cacgaaccac cccttacgat
    16741 ccttacggaa gattttggat tctgcctcga ggttcaacaa cgcctggcgc agcgtcatac
    16801 gtttaatgcc cagcagttct cccagctctc tttctgaagg gagttttcca cccggcgtgg
    16861 tgatgccatc ttttaaccac cgatccagtt tctcttttgc attttctacg gtggattgat
    16921 ttcccgtcat tgtttaatcc ttttggttta aaccagatga ggcatcttac gcttacgcca
    16981 aaaaagaaag caattaacgg cgtatgactt tgcagataat tgtgacaaag ctctctattg
    17041 acgtaatccc agacttataa gggagatact cgcattggtg tgcgctcacg acaggtaaaa
    17101 aaaaacctgc cagcgatggc aggtttttta taactaaaaa acgaaattaa tcgtctttcg
    17161 gtgcgcggtt ggcgcggcga cgatcgtttt ccgtcaggtg acgtttacga atacggatag
    17221 aggtcggagt cacttctacc agttcgtcat catcgatgaa ctccagagct tgttccagag
    17281 tcatgcggat aggcggaacc agaacaacgg cttcgtcagt accggaagca cgcatgttgg
    17341 tcagtttctt accggtcagg cagtttacag tcaggtcgtt agagcggcta tgaataccga
    17401 taatctgacc ttcgtaaact tctgcaccgt gaccgaggaa cagcttaccg cgatcctgca
    17461 gaccgaacag cgcgaacgcg accgctttac cctgaccgtt agagatcagt acgccgttct
    17521 gacgctgacc cacttcaccc ggacgtacgt cgtcgtagtg gctgaaggtg gagtacagca
    17581 gaccagtacc ggaagtcatg gtcatgaact cagaacggaa gccaatcaga ccacggcttg
    17641 ggatcacgta gtcgagacgt acgcggcctt taccgtctgg attcatgttt ttcaggtcgc
    17701 ctttacgttc gcccagcgcc tgcattacag aaccctgatg ctgttcttca acgtccagag
    17761 tcacgttttc atatggctct tgtttacgac cgtcgatttc acggaagata actttcggac
    17821 gggataccgc cagttcgaaa ccttcacgac gcatgttttc gatcagaaca gacaggtgta
    17881 gttcgccacg accagaaacg cggaacgcat cagcgtcttc ggtttcttct acgcgcagcg
    17941 caacgttgtg taccagttct ttgttcagac gatccaggat ctgacgagac gttacgaact
    18001 taccttcttt accgcagaac ggcgaggtgt taacgcagaa gaacatagaa acggtcggct
    18061 catcaacgga gagtgccggc agcgcttcaa cgttttgcgt gtcgcaaacg gtgtcagaaa
    18121 tgttcagttc gccaaggccc gtgatcgcaa cgatatcgcc agcttccgcc aggtcggttt
    18181 cgatacgttc cagaccgagg tggcccagca ctttaccgac tttcgcgttg cgggttttgc
    18241 cttcgctatc gatgatagtg acctgctggt tcggcttcac tttaccgcgc ttgatgcggc
    18301 caatgccgat aacgccaaca tagctgttgt aatcgagctg agaaatctgc atctggaacg
    18361 gaccgtcaag gtcaacgtcc ggcgcaggaa cgtggtcaac aatcgcctgg tacagcgggg
    18421 tcatgtcttc cgccatatct tcgtggtcca gacccgcgat accgttcagc gcagaagcgt
    18481 aaacgatcgg gaagtccagc tgctcgtcgg tcgcgtcgag gttaacgaac agatcgaata
    18541 cctgatccac aacccaatca ggacgcgcgc cagggcggtc aactttgttg ataacaacaa
    18601 tcggcttcag gccgtaagca aacgcttttt tggttacgaa gcgcgtttgc ggcatcgggc
    18661 cgtcaaatgc gtcaaccacc agcagcactg agtctaccat ggacattaca cgttcaactt
    18721 caccaccgaa gtcggcgtgc cccggggtat caacgatgtt gatacggtaa tcattccatt
    18781 tgatagcggt gtttttcgcg aggatggtaa tcccacgctc tttctccaaa tcgttggagt
    18841 ccatcacgcg ctcttgggtt tcggcacgag agtcgaacgt accggattgt tggagcagct
    18901 tgtctaccag ggtggtttta ccatggtcta cgtgcgcgat gatggcgata ttacgcaatt
    18961 tttcgatcac aactttgcct caggcattag aaatagcgcg ttattgtaca cggattaatc
    19021 gcactacaaa acaggatcac aaacatcctc cgcaaacaag tattgcagag tccctttgtg
    19081 atcgctttca cggagcataa aaagggttat ccaaaggtca ttgcaccaac atggtgctta
    19141 atgtttccat tgaagcacta tattggtgca acattcacat cgtggtgcag cccttttgca
    19201 cgatggtgcg catgataacg ccttttaggg gcaatttaaa agttggcaca gatttcgctt
    19261 tatctttttt acggcgacac ggccaaaata attgcagatt tcgttaccac gacgaccatg
    19321 accaatccag gagagttaaa gtatgtccgc tgaacacgta ctgacgatgc tgaacgagca
    19381 cgaagtgaag tttgttgatt tgcgcttcac cgatactaaa ggtaaagaac agcacgtcac
    19441 tatccctgct catcaggtga atgctgaatt cttcgaagaa ggcaaaatgt ttgacggctc
    19501 ctcgattggc ggctggaaag gcattaacga gtccgacatg gtgctgatgc cagacgcatc
    19561 caccgcagtg attgacccgt tcttcgccga ctccaccctg attatccgtt gcgacatcct
    19621 tgaacctggc accctgcaag gctatgaccg tgacccgcgc tccattgcga agcgcgccga
    19681 agattacctg cgttccactg gcattgccga caccgtactg ttcgggccag aacctgaatt
    19741 cttcctgttc gatgacatcc gtttcggatc atctatctcc ggttcccacg ttgctatcga
    19801 cgatatcgaa ggcgcatgga actcctccac ccaatacgaa ggtggtaaca aaggtcaccg
    19861 tccggcagtg aaaggcggtt acttcccggt tccaccggta gactcggctc aggatattcg
    19921 ttctgaaatg tgtctggtga tggaacagat gggtctggtg gttgaagccc atcaccacga
    19981 agtagcgact gctggtcaga acgaagtggc tacccgcttc aataccatga ccaaaaaagc
    20041 tgacgaaatt cagatctaca aatatgttgt gcacaacgta gcgcaccgct tcggtaaaac
    20101 cgcgaccttt atgccaaaac cgatgttcgg tgataacggc tccggtatgc actgccacat
    20161 gtctctgtct aaaaacggcg ttaacctgtt cgcaggcgac aaatacgcag gtctgtctga
    20221 gcaggcgctg tactacattg gcggcgtaat caaacacgct aaagcgatta acgccctggc
    20281 aaacccgacc accaactctt ataagcgtct ggtcccgggc tatgaagcac cggtaatgct
    20341 ggcttactct gcgcgtaacc gttctgcgtc tatccgtatt ccggtggttt cttctccgaa
    20401 agcacgtcgt atcgaagtac gtttcccgga tccggcagct aacccgtacc tgtgctttgc
    20461 tgccctgctg atggccggtc ttgatggtat caagaacaag atccatccgg gcgaagccat
    20521 ggacaaaaac ctgtatgacc tgccgccaga agaagcgaaa gagatcccac aggttgcagg
    20581 ctctctggaa gaagcactga acgaactgga tctggaccgc gagttcctga aagccggtgg
    20641 cgtgttcact gacgaagcaa ttgatgcgta catcgctctg cgtcgcgaag aagatgaccg
    20701 cgtgcgtatg actccgcatc cggtagagtt tgagctgtac tacagcgtct aatagttgaa
    20761 gttgtactac ccggcgcaac aacgccggga tttagttgcc gtggaaattt cagcccatct
    20821 ctgcatgggc ttttttctcc gtcaattctc tgatgcttcg cgctttttat ccgtaaaaag
    20881 ctataatgca ctaaaatggt gcaacctgtt caggagactg ctttatggca acaggcacgc
    20941 agcccgatgc tgggcagatc ctcaactcgc tgattaacag tattttgtta atcgatgaca
    21001 acctggcgat ccattacgcc aaccctgccg cgcaacaact gctcgcccaa agctcccgca
    21061 aattgtttgg tacaccgtta ccggaactgt tgagctactt ctcattaaat atcgagctga
    21121 tgcaagaaag tctggaggcg gggcaaggtt ttaccgataa cgaagtgacg ctggtcatcg
    21181 acgggcgctc gcatatcctt tctgtgacgg cccagcgtat gccggacggc atgatcctgc
    21241 tggagatggc tccgatggat aaccagcgcc gcttaagtca ggaacagcta cagcacgccc
    21301 agcaggttgc tgcccgtgat ttagtgcgcg gcctggcaca tgagattaaa aatccgcttg
    21361 gcggtttacg tggcgcggcg cagctgctca gcaaagcgtt acctgaccca tcactactcg
    21421 aatataccaa agtgattatc gaacaggcgg accggctgcg aaatctggtc gaccgtctgt
    21481 tggggccgca gctgcccggt acgcgcgtta ccgaaagtat tcacaaagtg gctgaacgcg
    21541 tggtaacgct ggtgtcgatg gaactgccgg acaacgtgcg gttgattcgt gattacgatc
    21601 ccagcctgcc ggaactggcg cacgacccgg atcaaattga acaggtcttg ctgaatattg
    21661 tgcgcaatgc gctacaggcg ctggggccgg aaggcggtga aatcattctg cgtacccgca
    21721 ccgcgtttca actgacctta cacggcgagc gctaccggct ggcggcgcgg attgatgtgg
    21781 aagataacgg gccgggcatt ccgcctcatt tgcaggatac gctgttttac ccgatggtca
    21841 gcggccgcga aggtggcacc gggcttggct tatccatcgc tcgtaatttg attgatcagc
    21901 attcaggcaa aattgaattt accagttggc cagggcatac cgagttctcg gtttacctgc
    21961 ctatcaggaa ataaaggtga cgtttatgca acgagggata gtctgggtag tcgatgacga
    22021 tagttccatc cgttgggtgc ttgaacgtgc gctcgctggg gcaggtttaa cctgtacgac
    22081 gtttgagaac ggcgcagaag tgctggaggc gctggcgagc aaaacgccgg atgtgctgct
    22141 ttcagatatc cgtatgccgg gaatggacgg gctggcgctg ctcaagcaga ttaaacagcg
    22201 ccatccaatg cttccggtca tcattatgac cgcacattcc gatctggatg ctgccgtcag
    22261 cgcctatcaa caaggggcgt ttgattatct gcccaaaccg tttgatatcg acgaagcagt
    22321 ggcgctggtt gagcgcgcta tcagtcatta ccaggaacag cagcagccgc gtaatgttca
    22381 gcttaacggc ccaacgaccg atatcatcgg cgaagcgcca gccatgcagg acgtgttccg
    22441 tattatcggt cggctttcgc gttcttctat tagcgtgctg attaacggcg aatccggcac
    22501 cggtaaagaa ctggtcgctc atgccctgca tcgccacagt ccgcgcgcca aagcgccgtt
    22561 tatcgcgctg aatatggcag ctatcccaaa agatttgatc gaatcagaac tgtttggcca
    22621 cgagaaaggc gcgtttactg gcgcgaatac cattcgtcag gggcgttttg aacaggccga
    22681 tggcggtaca ttattcctcg acgaaattgg tgatatgccg ctggatgtgc agacgcgttt
    22741 gctgcgcgtg ctggcagacg gtcagtttta ccgcgttggc ggctatgcgc cggtgaaagt
    22801 ggatgtgcgg attatcgctg ccactcacca gaatctcgaa cagcgagtgc aggaaggtaa
    22861 gttccgtgag gatctgttcc accgcctgaa cgttatccgc gttcatctgc cgccgctgcg
    22921 cgaacgtcgg gaagatattc cccgtctggc gcgccatttt ttacaggttg ccgcgcgcga
    22981 actgggcgta gaagcgaagt tactgcatcc ggaaaccgaa gctgctctga cgcgtctggc
    23041 gtggccaggc aacgtgcgcc agctggaaaa cacctgccgc tggctaacgg tgatggccgc
    23101 cgggcaggaa gtgttgattc aggatttgcc cggcgaactg tttgaatcaa cggttgcgga
    23161 gagtacttcg caaatgcaac cggacagttg ggcgacgctt cttgcgcagt gggcagacag
    23221 agcgctgcgt tccggtcatc aaaatctgct ttccgaagcg cagccagagc tggagcggac
    23281 gttactgacg accgcgttgc gacatacgca ggggcataaa caggaagcgg cgcggctact
    23341 cggctggggc cgcaacactc tgacgcgtaa gttaaaagag ctggggatgg agtgattcac
    23401 ggcgtgtgtg taaagattga ttattgagcg caaattgctg gtattttgcg ctttactgtt
    23461 ccgataagtt cagtatgatc ttgcccggaa aacggggaga gtcattatgc tggaatcaat
    23521 aattaatctg gtatcgagtg gcgcagttga cagccacaca ccgcaaactg ccgttgctgc
    23581 cgtgctgtgt gccgcaatga ttgggctgtt tagttgagtt tcagattgct gatgaagtgc
    23641 tctttgttca tgccggatgc ggcgtgaacg ccttatccgg cctacaaaag caatattttc
    23701 aacaggtttt gtaggcctga taagcgtagc gcatcaggca atcttgttct cagcacattg
    23761 atggcgcttt cgttttctac tttgtaaacg aagcgccatt cactatttaa atcacccgtg
    23821 agaactgctg catccgcgct ttctggcgca gataggtatc aaagcacatg caaatgttgc
    23881 ggatcagcaa gcgacctttc gccgtcacct gtattccctt ctcatccaca tccaccagcc
    23941 catcttttgc taacggggcg agcagcttga gatcttccgc aaagtaatca gcgaagtgca
    24001 aatcccactg tttctcaata ggggcgtaat ccagacggaa gttgcagatg agcgacttaa
    24061 tcacatcgcg gcgaatacag tcatcacgcg ttagcgcaat accacgccac agcgcattgc
    24121 cttgttcatc cacttgctga tagtactgct tcaactcttt ctggttctgc gcgtagcagt
    24181 cgccaatcat gctgatggcg gaaacgccca tccccagcag atcggtatcg ccctgagtgg
    24241 tgtagccctg gaagttacga tgcagcacgc cttcacgctg ggccaccgcc agctcgtcat
    24301 ccggacgggc aaagtgatcc ataccgataa actgatagcc cgattgcgtc aggaaggcga
    24361 tggtttcctg caggatatcg agtttttgct gcggactcgg caggtcagca tctttgattt
    24421 tgcgctgagc agcaaaaatg gtcggcagat gcgcgtagtt aaagacactc agacgatcgg
    24481 ggttcagctc cgccacacgt ttcagggtaa aggcgaaact ctccggcgtc tgtttcggca
    24541 ggccgtaaat caggtcgatg ttggtggagg taaaaccaat ctcacgcgca tggttaagca
    24601 gtgcaaagat gaactcttca tcctgctcgc ggttaaccag acgttgcact tctttgttga
    24661 agtcctgcac gcccatgctc aggcgattaa agccttcggc gcgtaaatga tcgagtacat
    24721 ccagttcgat ttcccgcgga tcgacttcga tcgaaatctc cgcatcggca ttgaactgga
    24781 agttttcgcg cagcagcttc atcaggcggc tgatttgcgc tttattcaga tacgtcggcg
    24841 ttccgccgcc ccagtgcaat tggctgacgt gacgcccggc gaacagcggt gcacgatgga
    24901 cgatttcttg ctccagcgcg tccagatact gatcggcctt gtgctgctgg cgagtaacaa
    24961 tcttattgca accgcagaag taacaaagct tatggcagaa cgggatatgt acgtagagag
    25021 ataatggacg ctcaggatag cgcgccacgg cttgtaaaaa cgcctgttcg ccgaagtctt
    25081 ctgaaaactc cagcgcggtc gggtacgagg tgtatcgtgg cccggaatag ttatatttct
    25141 ggatcagggc cagatcccag tcgatttgct gtacagacat gctcactcct tccgatggcg
    25201 tctctggcgg ctacggcgta ccggcggatg cccgtttcgg gtcatcagcc ggttgcgcag
    25261 ccacttttgt cgccgcgaca accgctgtag tttaacgaat aaccacacca gataacatat
    25321 aaccggaagg gttatcagca gaacgggaag gcccacggcg gcaatccgtt agttgccccg
    25381 cagcagacgc atcatgtctt cttgcttctc gtcttcttcc tcttcttcgt catcgtcata
    25441 agagaggccg agtttctgca tcaactcatc aatacgatcc agtttagcat ccacccagga
    25501 ttgctcttcg gcgctcaggg tttcgcctgc ttccagacgt tccagcagcg catccagacg
    25561 ctcatccgtt tccagtaatt ccaactccgc ctgcggtgaa agcataggtt tctcactctt
    25621 cggtttgtgc tgtttggtga ctttttcagt cacgcccaat ggaatagggg ttttactgcc
    25681 aatacgtgga tcttttggtg cgttctggcc tttgctgcct gacgtggtgt ttccgcccgc
    25741 tgcgcggctg cccggcgcat gaccacgacg ttttttctga cgcttgcgat cacgagcttc
    25801 ctgatccagc tcctcgcgtg tttttcgacg cgcttttgca tgacctttgc tgcgtgagtt
    25861 tgaagatgat ggtttcataa tggttcgtct tttagccgtt ttattcagta tagatttgcg
    25921 gcggaatcta acagaaagca agcaaagaaa aaaggcgaca gattactctg tcgccttttt
    25981 tcctgactca taacccttaa cgggtcttac aatccttgca ggccgccaac acaccctgtt
    26041 tttcctttag ccggaacaat ccgtgttgat tccatttccg tttaattacg tctccggacg
    26101 tttgtcttcc tgacaatcct ctgtcttcgc ctcctggcgc tcctgaccct catcctgagt
    26161 cattgttcct gttagcgtcc tcgctctatg ggtgctactt tacgcctttg ctttaaacaa
    26221 acaatcaacc gccatcagta tttgcacatt ttcagagttt tatataaaag caattcactg
    26281 atttatatag cttatttgac atgcatcgca gaattttctc tgctatttcg cataaagact
    26341 atcggcaatg tctcacaaag cgtatggctt ttacttacaa cgctcacgcc gaaaaatccg
    26401 ccttttatta tggttcaggt ataatcgcca caaagctttg actgacggag acgaccgctt
    26461 tgactaattt gaattatcaa cagacgcatt ttgtgatgag tgcgcctgat attcgccacc
    26521 taccttccga taccggaatt gaagtggctt ttgcaggccg ttccaacgca ggtaaatcca
    26581 gcgcgctgaa cacgctgact aaccagaaaa gcctggctcg tacctcaaaa accccagggc
    26641 gcacccagct tatcaacctg tttgaagtgg ctgacggcaa gcgtctggtt gacttgcctg
    26701 ggtacggtta tgcggaagtc ccggaagaga tgaagcgcaa atggcagcgt gcgctcggcg
    26761 aatacctcga aaaacgtcag agcctgcaag gtctggtggt gctaatggat attcgccatc
    26821 cgctgaaaga tttggatcag cagatgattg agtgggcggt agacagcaat atcgccgttc
    26881 tggtgctgct gaccaaagcg gacaaactgg caagcggcgc acgtaaagcg caattgaata
    26941 tggtgcgtga agctgtactg gcgtttaacg gtgatgtgca ggttgaaacg ttttcttcgt
    27001 tgaagaaaca aggcgtggac aagctgcggc agaaactgga tacctggttt agcgagatgc
    27061 agcctgtaga agaaacgcag gacggcgaat aattttcttg ccttaatgct tgtgccggat
    27121 gtggcgtatc cggcccgtaa attcaataga ttacaattct accgttggcc tgataaacat
    27181 agcgcatcag gcattacgga tcttttcttt cgcccaataa aaaacgcccc agtcattact
    27241 gactggggcg gctaaaatat tcagccaaat ccgattacgt gaagtaaaag gtctgaaaga
    27301 tagaacatct tacctctgta ccctacgcga ctaactttac tcttttttgt tcagttcaga
    27361 aagcactttt tgtaattttt tttcagttgt tgcataggaa tttctaatag ccatcacaaa
    27421 acgtgacagc ttatgttgct tacttacgaa aaaagggcat gttcaggcga atcttagtgc
    27481 gcctgatccc agttttcgcc actccccact tccaccagca acggcacatc cagacgggta
    27541 cagttttcca tcagttgatg aatctgcttc gcgacggcat caacatcatc tttatgaact
    27601 tcaaatacca gttcatcgtg tacctgcatg atcatacgta cacgcggttg ctcagcctgt
    27661 aaccacgcat caacggcaat catcgcccgt ttgataatgt cggcggcggt tccctgcatt
    27721 ggcgcgttaa tggctgcacg ttcagccgct gcacgacgag caccattgct ggatttgata
    27781 tccggcagat acagacggcg tccgtccagc gtttcaacgt agccctgctc tttcgcctga
    27841 gcacgggtgc gttccatata ctccagcacg ccagggtagc gttcgaagta aaggtccatg
    27901 tacttctgcg cttctttacg tggaatgttc aattgccgcg ccagaccgaa agcactcatg
    27961 ccataaatca gaccaaagtt gatcgctttc gcgctacggc gttgctcgct ggtgacggtt
    28021 tccagtggca aaccaaacac ttctgccgcc gttgcccggt ggatatcttt tccttccgcg
    28081 aatgcggtca gcaagccttt gtcacgcgag agatgcgcca taatgcgcag ttcaatctgc
    28141 gagtagtccg ctgagacaat cacataatcc tctggcgcaa taaacgcctg gcggatacga
    28201 cgaccttctt cgttacgcac cggaatgttt tgcaggttag gatcggttga cgataaacgt
    28261 cccgttgcag ttactgcctg gtgataagag gtatgcacac gcccggtttt cgggttgatc
    28321 atcagcggca gcttgtcggt gtaggtcgat ttcagcttcg ccagaccacg atactccaga
    28381 atcacttttg gcaacggata gtccagcgcc agttcttcca gtacctcttc cgacgttgac
    28441 ggcgcgccac ccggcgtttt cttcagcggt ttaataccct gtttttcaaa cagaatggtt
    28501 tgcaactgct tggtggaaga aaggttaaat tcttcacctg caatttcatg cgctttcttt
    28561 tccagctcag ccagacgcaa ggtgagctct tcagaatgat tgtgcagcac tttcggatcg
    28621 atcttcacac cgttacgttc aatgcgtgaa agcaccggca ccagcggcat ttcgatattc
    28681 tcgaagacgt tcaacggccc tttgtgtttt tgcaggtccg gccacatttt cagatgcaac
    28741 tgcaaggtga catcagcatc ttcggcggcg taacgtccgg cctcttcgag ggcaatctgg
    28801 ttaaaggtca gttgattttt gcctttaccc gcaatctctt caaaagtgat ggttttgtgc
    28861 ttcaaccaac gttccgcgag gctgtccata tcgtgacgcc cggcaacgct attgagaatg
    28921 taggactcca gcatggtatc aaacgcaatc ccacgcagct caatgccgta attcgccaga
    28981 ataccgcgat cgtatttcag gttttgcccg accttcagcg ccttttcatc ttccagcagc
    29041 ggttttagca actcgagtgc acgctcgcga gagatttgat cgggcgcatc aagataatca
    29101 tgagcaaccg gaatatatgc cgctacgcct ggctcgatag caaaagaaag cccgaccagg
    29161 ttagcagaga tgttatcaag gctgtcggtt tcggtatcaa atgcaaatac cggcgctttt
    29221 tccagcttcg caatccacgc tttcagtgtt tcttcatcaa ggatggtgac gtagttgtca
    29281 taagaaatca ccgttgccgt cacttctggc gcttcgtctg caacactggt ttcctgcggc
    29341 ttcgcggctg gttttgcccc tttggcctgt aaccatttgc ccgcttcgac atcagcagtc
    29401 cagcgtttga actcatactt tttgaacagc cccaacaact cttccgctgc cggttgctgc
    29461 acttccagtt gttcacaggt cagctccagt tcaacgtcgg ttttaatcgt cgccagctgg
    29521 tatgagagat aagcaacttc tttgttttgc tcgagcttcg ctgccattgt tttcgcgcca
    29581 cggaagctca acccagcaat tttttctggc tcggcataca gcgtatccag cccgcccaga
    29641 ccttgcagca atgcctgcgc ggttttttca ccgacgcccg gtacgccagg gatgttatcg
    29701 gaggagtcac ccatcagcgc caggaaatcg atgatcagtt ctggcggcac gccgtactta
    29761 ttcaccacct cttccggtcc gaggatggta ttcgtcatgg tattgataag cgtaatattt
    29821 ggcgtcacca gctgcgccat atctttatcg ccagtgctga tcagcaccgg acgcccggct
    29881 ttttcggctt cgcgcgccag agtaccgata acgtcgtccg cttctacgcc agaaaccgcc
    29941 agcagcggca gtcccatcgc tttaaccatc gcgtgcaagg gttcgatttg tgcacgcaga
    30001 tcgtccggca ttggcgggcg atgtgatttg taatgttcaa acagttcatc acgaaaggtt
    30061 tttcccttgg cgtcaaagac caccgctgca tgcgtcggtt tatattgcat gatcagactg
    30121 cgcagcatgt tgaggacacc atacatcgca ccggtcggct cgcctgcgct gttagtcagc
    30181 gggggaaacg cgtgatatgc gcgataaaga taagatgaac catctacaag gataagtgga
    30241 ttttggggga tctgaaccat aatgtccgtg cctgtttatc agattatggg taaaggatgc
    30301 cacagacagg atgaaaacat gagtttttca ccgcttttcg tgaaaatctt ttgtagatct
    30361 tctggatcgc tcgcaaatcg tcatgtggat aactttgtgc ataaattatt tacatcgctt
    30421 ttaatttatt atcatgcttt ttacagcatg atgtttgtct ttgtttttca tgcagttaaa
    30481 aataaatcca tccctaataa ttgagataat gggatttttt ctaatgtgga tagatatgaa
    30541 ttatttttct ccttaaggat catccgttat ttgggtcgtt ttctgctaaa atctgcgcct
    30601 tgcgcttctt tagcgcactg tctatggcta accttttgaa taaattcatt atgacgagaa
    30661 tactcgctgc gataaccctt ttgctgagta tcgtattgac cattttggtc actatttttt
    30721 gttccgtccc gatcatcatt gccgggattg taaaactatt gctacctgtg ccagtcattt
    30781 ggcgaaaggt ttcgcgtttt tgtgatttta tgatgtattg ctggtgtgaa ggtctggcgg
    30841 tattactgca tctgaaccca cacctgcaat gggaagttca cgggctggaa gggttaagta
    30901 agaagaactg gtatctgctt atttgtaacc accgtagttg ggcagatatt gtcgtactgt
    30961 gcgtgctgtt tcgtaagcac attccgatga ataaatattt cctcaagcaa cagctggcct
    31021 gggtgccatt tcttggcctg gcgtgctggt cgctggatat gccgtttatg aagcgttatt
    31081 cccgcgctta tttattacgt catccggagc gacgtggtaa agatgttgaa accactcggc
    31141 gttcttgtga aaagtttcgc ctgcatccca ccaccattgt caatttcgtt gaaggctccc
    31201 gtttcacgca agaaaaacat cagcaaaccc actccacttt tcaaaacttg ttgccaccaa
    31261 aggcggcagg cattgcgatg gcgctaaatg tactgggcaa acaattcgat aaactgctta
    31321 atgttacact gtgttatccg gacaataacc gtcagccgtt cttcgatatg ttaagcggta
    31381 aactgacgcg gattgtcgta catgtggatt tacagcctat tgccgatgag ttacatggcg
    31441 actacatcaa tgacaaaagc tttaagcgcc attttcagca atggctgaac tcattgtggc
    31501 aggaaaaaga ccggcttctg acgtctctca tgtcatcaca gcgtcagaat aagtaagttt
    31561 tactcgcctc tgaatgagca gaggcgagtg agtattttaa tgaacaaaac gtccggcacg
    31621 agacataaat tcttcttcgc tcatctcctg tccgttaaaa acaactttac ccggtgtata
    31681 acgcagctgc agcgaggcgg tattgttgtc aatcgtgatt aactgaaaca tctgacccaa
    31741 tgtcatcatc ccgctgattt gtttatcagc ctgcttttgc gctttttccg catccattcc
    31801 ttcagataaa ttaagctgtt ttgcggtttc tgtgacgaca ttaagcggta acttcacatc
    31861 aaaattgagc gatttgatat ctttgtttgt ggatgatgaa gatttggctg ggtcggcaat
    31921 actgatatcc agattggcat ttagttcgcc gagtgcgttc ttccatctta ccggttgttt
    31981 aatggtcggc tcactttttt gtaataacgg taaatactct ttgaacaatg cagcattcac
    32041 ttcttgcaga gcaacttcat cgtttgctaa ctcagggtgt acagcaagct gcttctgcat
    32101 agcaatgtta tactggatga taaattggcg cactgctgag ggatcaatag attcagcaat
    32161 caatgaccac tcaccactac cgagttgctg attccctttt gttaacttat caagttcgta
    32221 ggtcagattg acattgatat aatctttgtc tttaacgcga tccagcatgg tttttgcaac
    32281 gaaagcatcg atttgggcaa cgtcttcccc gtgattgata atgttaatct gattaaattt
    32341 cgactcgctt tcaccaaccg gaaatttctt ttcttctaat cgcgccagag aatcaatggt
    32401 aaaacttttt gccgttactt tgttcattgg tgataattgc agaacaatat cttcgacttt
    32461 gccttcaata tccaggttag acaatgaact accatcacca ttaaaagtaa actggccctc
    32521 accaaaagaa aactttccat actctgcagg ggcaacatct aatgtcgtag agaatgagcc
    32581 accaaacgcg aatcgcaatg tcgcttccac gggcgattta ttttttgcgg cgataaatag
    32641 cggttgtgtc agttcattat tcactaagtt cacttttgcc gctgccagtg ctgggatgac
    32701 attaccatgc attaacattg tgatgggcaa cggaccgtgc tcaacatcca catcaaatac
    32761 aactttctgg cctggcttga tattgagatc gggtgcaccg ttatcgaatg tcatttgcat
    32821 ttgaaaacgg gaattaaaaa agcctttgtc aaaattttga taacttaacg tcatatcgta
    32881 ggcatgttca cctttctttt tagcatcgtt aaaatcttta ataaattttt cgacaccagg
    32941 ctgaatttgt gtacctgtgt accatgtgcc accaccccag attacggcta acgcaacaat
    33001 aacacctgta gctgacttac gtatcatata aatattccct gataaaacag caaaactttg
    33061 aatatccact tatgctgaaa ta
//
LOCUS       DAZGVQ010000038        32624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000038 DAZGVQ010000000
VERSION     DAZGVQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.038"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(53..214)
                     /gene="rsmS"
                     /locus_tag="ACHLAG_001767"
     CDS             complement(53..214)
                     /gene="rsmS"
                     /locus_tag="ACHLAG_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFP0655013.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(228..755)
                     /gene="priC"
                     /locus_tag="ACHLAG_001768"
     CDS             complement(228..755)
                     /gene="priC"
                     /locus_tag="ACHLAG_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFP0655014.1"
                     /translation="MKTALLLEKVEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLTRVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            825..1202
                     /gene="ybaN"
                     /locus_tag="ACHLAG_001769"
     CDS             825..1202
                     /gene="ybaN"
                     /locus_tag="ACHLAG_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFP0655015.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            1355..1906
                     /gene="apt"
                     /locus_tag="ACHLAG_001770"
     CDS             1355..1906
                     /gene="apt"
                     /locus_tag="ACHLAG_001770"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFP0655016.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEFHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVTFPGH"
     gene            2035..3966
                     /gene="dnaX"
                     /locus_tag="ACHLAG_001771"
     CDS             2035..3966
                     /gene="dnaX"
                     /locus_tag="ACHLAG_001771"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFP0655017.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    3299..3363
                     /gene="dnaX"
                     /locus_tag="ACHLAG_001771"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            4019..4348
                     /gene="ybaB"
                     /locus_tag="ACHLAG_001772"
     CDS             4019..4348
                     /gene="ybaB"
                     /locus_tag="ACHLAG_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFP0655018.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            4348..4953
                     /gene="recR"
                     /locus_tag="ACHLAG_001773"
     CDS             4348..4953
                     /gene="recR"
                     /locus_tag="ACHLAG_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFP0655019.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            5063..6937
                     /gene="htpG"
                     /locus_tag="ACHLAG_001774"
     CDS             5063..6937
                     /gene="htpG"
                     /locus_tag="ACHLAG_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFP0655020.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            7118..7762
                     /gene="adk"
                     /locus_tag="ACHLAG_001775"
     CDS             7118..7762
                     /gene="adk"
                     /locus_tag="ACHLAG_001775"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFP0655021.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            7998..8960
                     /gene="hemH"
                     /locus_tag="ACHLAG_001776"
     CDS             7998..8960
                     /gene="hemH"
                     /locus_tag="ACHLAG_001776"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFP0655022.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(8957..9916)
                     /gene="aes"
                     /locus_tag="ACHLAG_001777"
     CDS             complement(8957..9916)
                     /gene="aes"
                     /locus_tag="ACHLAG_001777"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFP0655023.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQSDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            10068..11372
                     /gene="gsk"
                     /locus_tag="ACHLAG_001778"
     CDS             10068..11372
                     /gene="gsk"
                     /locus_tag="ACHLAG_001778"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFP0655024.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(11505..13181)
                     /gene="ybaL"
                     /locus_tag="ACHLAG_001779"
     CDS             complement(11505..13181)
                     /gene="ybaL"
                     /locus_tag="ACHLAG_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFP0655025.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARVKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(13419..14639)
                     /gene="fsr"
                     /locus_tag="ACHLAG_001780"
     CDS             complement(13419..14639)
                     /gene="fsr"
                     /locus_tag="ACHLAG_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFP0655026.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            14857..16509
                     /gene="ushA"
                     /locus_tag="ACHLAG_001781"
     CDS             14857..16509
                     /gene="ushA"
                     /locus_tag="ACHLAG_001781"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP0655027.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(16546..17025)
                     /gene="ybaK"
                     /locus_tag="ACHLAG_001782"
     CDS             complement(16546..17025)
                     /gene="ybaK"
                     /locus_tag="ACHLAG_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFP0655028.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(17229..18023)
                     /gene="ybaP"
                     /locus_tag="ACHLAG_001783"
     CDS             complement(17229..18023)
                     /gene="ybaP"
                     /locus_tag="ACHLAG_001783"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFP0655029.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            18161..18502
                     /gene="ybaQ"
                     /locus_tag="ACHLAG_001784"
     CDS             18161..18502
                     /gene="ybaQ"
                     /locus_tag="ACHLAG_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP0655030.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(18616..21120)
                     /gene="copA"
                     /locus_tag="ACHLAG_001785"
     CDS             complement(18616..21120)
                     /gene="copA"
                     /locus_tag="ACHLAG_001785"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFP0655031.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLLMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            21382..22314
                     /gene="glsA"
                     /locus_tag="ACHLAG_001786"
     CDS             21382..22314
                     /gene="glsA"
                     /locus_tag="ACHLAG_001786"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFP0655032.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            22317..23609
                     /gene="ybaT"
                     /locus_tag="ACHLAG_001787"
     CDS             22317..23609
                     /gene="ybaT"
                     /locus_tag="ACHLAG_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP0655033.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            23640..23737
                     /locus_tag="ACHLAG_001788"
                     /pseudo
     CDS             23640..23737
                     /locus_tag="ACHLAG_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23734..24141
                     /gene="cueR"
                     /locus_tag="ACHLAG_001789"
     CDS             23734..24141
                     /gene="cueR"
                     /locus_tag="ACHLAG_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFP0655034.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(24142..24600)
                     /gene="ybbJ"
                     /locus_tag="ACHLAG_001790"
     CDS             complement(24142..24600)
                     /gene="ybbJ"
                     /locus_tag="ACHLAG_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFP0655035.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVSAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(24597..25514)
                     /gene="qmcA"
                     /locus_tag="ACHLAG_001791"
     CDS             complement(24597..25514)
                     /gene="qmcA"
                     /locus_tag="ACHLAG_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP0655036.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            25660..26337
                     /gene="fetA"
                     /locus_tag="ACHLAG_001792"
     CDS             25660..26337
                     /gene="fetA"
                     /locus_tag="ACHLAG_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFP0655037.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            26324..27103
                     /gene="fetB"
                     /locus_tag="ACHLAG_001793"
     CDS             26324..27103
                     /gene="fetB"
                     /locus_tag="ACHLAG_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFP0655038.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(27166..28020)
                     /gene="cnoX"
                     /locus_tag="ACHLAG_001794"
     CDS             complement(27166..28020)
                     /gene="cnoX"
                     /locus_tag="ACHLAG_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFP0655039.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQESNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(28081..28890)
                     /gene="ybbO"
                     /locus_tag="ACHLAG_001795"
     CDS             complement(28081..28890)
                     /gene="ybbO"
                     /locus_tag="ACHLAG_001795"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP0655040.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(28880..29503)
                     /gene="tesA"
                     /locus_tag="ACHLAG_001796"
     CDS             complement(28880..29503)
                     /gene="tesA"
                     /locus_tag="ACHLAG_001796"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFP0655041.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            29474..30160
                     /gene="ybbA"
                     /locus_tag="ACHLAG_001797"
     CDS             29474..30160
                     /gene="ybbA"
                     /locus_tag="ACHLAG_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFP0655042.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            30157..32571
                     /gene="ybbP"
                     /locus_tag="ACHLAG_001798"
     CDS             30157..32571
                     /gene="ybbP"
                     /locus_tag="ACHLAG_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFP0655043.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKQIVGQWLMVLALSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVEPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         7738 a         8620 c         8795 g         7471 t
ORIGIN      
        1 gttttgcgtt tgcccacaat cttaggggac atttagcggc cccatttatt tctcactttt
       61 ccgcctcatc atcgcgcgtt aatttctttt cataatcgcg ttttacaata tccagcgcgc
      121 gcagcacggt gcgggctggg atctgatttt cttccagcag cacaatcaaa tcgacagcca
      181 gtttgacatc gtcaggggca ttttccagtg acatactctc tccattgcta gcgggttaag
      241 cgcgctagcc tgttttcgat tttttccagc gcatggcggc agcgtgccag gcgcgcttca
      301 taggcttcca cttcacgatg cagcgtttgc tgttccacga gatcggtcac ccgcgtcagg
      361 cgggctctgc gttcggcaac catctcacgt agccgccgct caaaatcctg atgctgaata
      421 cgtttacgct gccagcgggc aattttcggt ggcgcactgt cccactcgcg caatgaccag
      481 gcggaggctt cacgcgcgat ggcttccagt tgtgccgcca gatgttccgc cagccaggcc
      541 acttgcggca gctgttgctg ctcaactgca tgacgaagcg cagccagatt atcgcccgcc
      601 tcatcgagac aagcctgcag tgttgtcgca cgggtttgaa aaaggtgtct gtcaaaacgg
      661 gcacttagcg tggcaaattg cgctaccggg gcacaacgct gacgcagcgt agcgagctgt
      721 ccttccactt tttccagcag cagggcggtt ttcacgataa gacctcaatg aaaatgataa
      781 ttgttatgct aaagtagcca ctctgtaagc tgacaacact caatatgcaa cgaatcattt
      841 taatcatcat tggctggctg gcggtagtgc tgggtacgct gggcgtggta ttaccggtat
      901 taccgacgac gccgtttatc ctgctggcgg cctggtgctt tgcccgttct tccccgcgct
      961 ttcacgcttg gttgctgtac cgctcatggt ttggcagcta tctacgtttc tggcagaaac
     1021 atcatgcgat gccgcgcggc gtcaaaccgc gggcgatttt gcttattttg ctcacgtttg
     1081 ccatttctct gtggttcgtc cagatgccat gggtgcgcat catgttgctg gtaattctcg
     1141 cctgtttgct tttctatatg tggcgaattc cggtgattga tgaaaagcaa gaaaagcact
     1201 gaagcacaac aatcgcagtt gcaattattg cgtacagcca gtacattctg gcgttttcga
     1261 gcacaggcgc aggcggtcaa aggttaaaca actgttactt ttgatacgtt taaaacgcgc
     1321 cgtgagtacc accgtaacaa gcaggcatac acttatgacc gcgactgcac agcagcttga
     1381 gtatctcaaa aatagcatca aaagcattca ggactaccca aaacccggca ttcttttccg
     1441 cgatgtcacc agcttactgg aagacccgaa agcttacgct ctcagcatcg acttgctggt
     1501 tgagcgttac aaaaatgcgg gcattaccaa agttgtcggc accgaagcgc gtggcttctt
     1561 gtttggcgct ccggtagctc tgggtctggg cgttggcttt gtaccggtcc gtaaaccggg
     1621 caaactgccg cgtgaaacca tcagtgaaac ttacgacctg gaatacggca ccgatcagct
     1681 ggagttccac gttgatgcca tcaaaccggg cgacaaagtt ctggtggtgg acgatctgct
     1741 ggcaaccggc ggcactatcg aagcgaccgt taaactgatc cgtcgtctgg gtggtgaagt
     1801 ggctgacgct gcgttcatta tcaacctgtt cgatctcggc ggcgaacagc gtctcgaaaa
     1861 acagggcatt accagctaca gccttgtcac gttcccgggc cattaattat cgccagtctt
     1921 gtgctgccca cgctacggac agcacaagat gtgcattcag cctcgccgtt ctgacggggc
     1981 tgtgttagca ttaccccttc gtgaatccac cttccagcgt ttcagagcct gccaatgagt
     2041 tatcaggtct tagcccgaaa atggcgccca caaacctttg ctgacgtcgt cggccaggaa
     2101 catgtgctga ccgcactggc gaacggcttg tcgttagggc gtattcatca tgcttatctt
     2161 ttttccggta cccgtggcgt cggaaaaacc tctatcgccc gactgctggc gaaggggcta
     2221 aactgcgaaa ccggcattac cgcgacgccg tgcggcgtgt gcgataactg tcgtgaaatc
     2281 gagcaggggc gctttgtcga tctgattgaa atcgacgccg cctcgcgcac caaagttgaa
     2341 gatacccgcg acctgctgga taacgtccag tacgctccgg cgcgtggtcg tttcaaagtt
     2401 tatctgatcg acgaagtgca tatgctgtcg cgccacagct ttaacgcact gttaaaaacc
     2461 cttgaagagc cgccggagca cgttaagttt ctgctggcga cgaccgatcc acagaaattg
     2521 ccggtgacga ttttgtcgcg ctgtttgcaa tttcatctca aggcgctgga tgtcgagcaa
     2581 attcgccatc aacttgagca catcctcaac gaagaacata tcgctcacga gccgcgggcg
     2641 ttgcaattgc tggcgcgcgc cgctgaaggc agcctgcgag atgccttaag tctgaccgac
     2701 caggcgattg ccagcggtga cggccaggtt tcaacccagg cagtcagtgc gatgctgggt
     2761 acgcttgacg acgatcaggc gctgtcgctg gttgaagcga tggtcgaggc caacggcgag
     2821 cgcgtaatgg cgctgattaa tgaagccgct gcccgtggta tcgagtggga agcgttgctg
     2881 gtggaaatgc tcggcctgtt gcatcgtatt gcgatggtac aactttcgcc tgctgcactt
     2941 ggcaacgaca tggccgccat tgagctgcgg atgcgtgaac tggcgcgcac cataccgccg
     3001 acggatattc agctttacta tcagacgctg ttgattggtc gcaaagaatt accgtatgcg
     3061 ccggaccgcc gcatgggcgt tgagatgacg ctgctgcgcg cgctggcgtt ccatccacgt
     3121 atgcctctgc ctgagccaga agtgccacgc cagtcctttg cgcccgtcgc gccaacggca
     3181 gtaatgacgc caacccaggt gccgccgcaa ccgcaatcag cgccgcagca ggcaccgact
     3241 gtaccgctcc cggaaaccac cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg
     3301 cagggagcaa ccaaagcaaa aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg
     3361 aataacgctg cgctggaaag actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg
     3421 ccatcggcgc tggaaaaagc gccagccaaa aaagaagcgt atcgctggaa ggctaccact
     3481 ccagtgatgc agcaaaaaga agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa
     3541 catgaaaaaa cgccggaact ggcggcgaag ctggcggcag aagccattga gcgcgacgcg
     3601 tgggcggcac aggtgagtca actttcgcta ccaaaactgg tcgaacaggt ggccttaaat
     3661 gcctggaaag aggagagcga caacgcagta tgtctgcatt tgcgctcctc tcagcggcat
     3721 ttgaacaacc gcggtgcaca gcaaaaactg gctgaagcgt tgagcacgtt aaaaggttca
     3781 acggttgaac tgactatcgt tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg
     3841 cgtcaggcga tatacgaaga aaaacttgcg caggcgcgcg agtccattat tgcggataat
     3901 aatattcaga ccctgcgtcg gttcttcgat gcggagctgg atgaagaaag tatccgcccc
     3961 atttgatcgt aagcacagct tacgttcgtc atccttaacg tgattgagag agaaacctat
     4021 gtttggtaaa ggcggtctgg gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat
     4081 gcagaaaatg caggaagaga tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct
     4141 ggtaaaagtg accatcaacg gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct
     4201 gctggaagac gacaaagaga tgctggaaga cctggtggct gcagcattca acgacgcagc
     4261 acgtcgtatt gaagaaacgc agaaagaaaa aatggcctct gtatcctccg gaatgcagct
     4321 gccgcctggc tttaagatgc cgttctgatg caaaccagcc cgctgttaac acagcttatg
     4381 gaagcactgc gctgtctgcc gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg
     4441 ctgcttcagc gcgatcgtag cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg
     4501 tcggaaatcg gccactgcgc cgattgccgc actttcaccg aacaggaagt ctgtaacatc
     4561 tgttcgaatc cgcgtcgtca ggaaaacggt caaatctgtg tggtggagag tccggcggac
     4621 atctacgcca ttgagcagac ggggcagttt tcaggccgtt attttgtgtt gatggggcac
     4681 ctgtcaccgc tggacggcat cggtccggat gatatcggcc ttgatcgtct ggaacagcgt
     4741 ctggcagagg aaaaaatcac tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa
     4801 gctaccgcta actacattgc cgagctttgc gcgcaatatg acgtggaagc cagccgaatc
     4861 gctcatggcg taccggttgg cggcgaactg gaaatggtcg atggcaccac gctgtcacac
     4921 tcccttgccg ggcgtcataa gattcgtttt taagcaaacg agagcaggat cacctgctct
     4981 cgcttgaaat tattctccct tgtccccatc tctccctcat cctgttttta accttaaaat
     5041 ggcattattg aggtagacct acatgaaagg acaagaaact cgtggttttc agtcagaagt
     5101 gaaacagctt ctgcacctga tgatccattc tctctattcc aataaagaaa tcttcctgcg
     5161 tgagcttatc tctaacgcct ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa
     5221 cccggacctg tacgaaggtg atggcgaact gcgcgttcgt gtctctttcg ataaagacaa
     5281 gcgcacgctg accatctccg ataacggcgt ggggatgacc cgcgacgaag tgattgacca
     5341 tctggggact atcgctaaat ccggtaccaa atcattcctc gaatccctgg gttctgacca
     5401 ggcgaaagac agccagctga tcggtcagtt tggtgttggt ttctactctg cgtttatcgt
     5461 ggccgacaaa gtgaccgtgc gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt
     5521 ctgggaatcg gctggcgaag gtgaatatac cgttgccgac atcaccaaag aagatcgtgg
     5581 tactgaaatc accctgcatc tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt
     5641 gcgttccatc atcagcaaat actccgacca tatcgcgctg ccggtagaga tcgaaaaacg
     5701 cgaagagaaa gacggcgaaa ccgttatctc ctgggagaaa atcaacaaag cgcaggcgct
     5761 gtggactcgt aacaagtcgg aaatcaccga tgaagagtac aaagagttct acaaacacat
     5821 cgcccacgac tttaatgatc cgctgacctg gagccacaac cgcgttgaag gtaagcagga
     5881 gtacaccagc ctgctgtaca tcccgtccca ggctccgtgg gatatgtgga accgtgatca
     5941 taaacacggc ctgaaactgt acgtccagcg tgtgttcatc atggacgacg cagaacagtt
     6001 catgccgaac tatctgcgct tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa
     6061 cgtttcccgt gaaatcctcc aggacagcac ggtaacgcgt aacctgcgca atgcgctgac
     6121 caagcgtgtg ctgcaaatgc tggaaaaact ggcgaaagac gacgcggaaa aataccagac
     6181 cttctggcaa cagtttggcc tggtactgaa agaaggtccg gcggaagatt tcgcgaacca
     6241 ggaagcgatc gccaaactgc tgcgttttgc ttctacccat accgattctt ctgcgcagac
     6301 cgtatctctg gaagactacg tttcccgcat gaaagaaggg caggagaaaa tctactacat
     6361 caccgcagac agctatgcgg cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa
     6421 aggcatcgaa gttctgctgc tttccgaccg catcgatgag tggatgatga actatctgac
     6481 tgagttcgac ggtaaaccgt tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact
     6541 ggctgacgaa gttgatgaga gtgcgaaaga agcggagaaa gcactgactc cgttcatcga
     6601 ccgtgtgaaa gccctgctcg gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac
     6661 cgatacgcca gcgatcgttt cgaccgacgc ggacgaaatg agcactcaga tggctaaact
     6721 gttcgctgcg gcgggccaga aagtgccaga agtgaaatac atcttcgaac tgaacccgga
     6781 tcacgtactg gtgaaacgtg cggcagatac tgaagatgaa gcgaagttca gcgagtgggt
     6841 agaactgctg ctggatcagg cgctgctggc agaacgcggc acgctggaag atccgaacct
     6901 gtttattcgt cgtatgaacc agctgctggt ttcctgatgt catgccggat gacctttgtg
     6961 tcatccggca tttttctttt catcatctgc actttccgca aattatctcg ccattaaccg
     7021 tttcagcctc aggtgccttt cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt
     7081 ttcaaaaaat tcgacacatt ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc
     7141 tccgggcgcg gggaaaggga ctcaggctca gttcatcatg gagaaatatg gtattccgca
     7201 aatctccact ggcgatatgc tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca
     7261 agcaaaagac attatggatg ctggcaaact ggtcaccgac gaactggtga tcgcgctggt
     7321 taaagagcgc attgctcagg aagactgccg taatggtttc ctgttggacg gcttcccgcg
     7381 taccattccg caggcagacg cgatgaaaga agcgggcatc aatgttgatt acgttctgga
     7441 attcgacgta ccggacgaac tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc
     7501 gtctggtcgt gtttatcacg ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt
     7561 taccggtgaa gaactgacta cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct
     7621 ggttgaatac catcagatga ctgcaccgct gatcggctac tactccaaag aagcggaagc
     7681 gggtaacacc aaatacgcga aagttgacgg caccaagccg gttgctgaag ttcgcgctga
     7741 tctggaaaaa atcctcggct aattcgaaag cgcgcacgga cagtcccctc gccccctcgg
     7801 ggagagggtt agggtgaggg gaacaggccc gcacaagcaa acttatcagc aatctcaggc
     7861 cggatattca ttcggccttt tacaaaaacc tcaatctgcg ctattccttt ttctgatttg
     7921 acctctcaca gcaattagtt cttcttcctc acttttccgc tacaattatc aacaagttga
     7981 atcgataaga ggcggtaatg cgtcagacta aaaccggtat cctgctggca aacctgggta
     8041 cgcccgatgc ccccacacct gaagcggtaa aacgctatct gaaacaattt ttaagcgaca
     8101 gacgcgtggt tgatacctca cggttgttat ggtggccgtt gctgcgcggc gtgattttgc
     8161 cgctgcgctc gccgcgtgtg gcgaagctgt acgcctcggt ctggatggaa gacggctcgc
     8221 cgctgatggt ttacagccgc cagcaacagc aggcgctggc acaacgttta ccggagacgc
     8281 ccgtagcgct gggcatgagc tacggctcgc catcactgga aagcgccgtc gatgaactcc
     8341 tggcagagca tgtagatcat attgtggtgc tgccgcttta tccgcaattc tcctgttcta
     8401 cggtcggtgc ggtatgggat gaactggcac gcattctggc gcgcaaacgt agcattccgg
     8461 ggatatcgtt tattcgtgat tacgccgata accacgatta cattaatgca ctggcgaaca
     8521 gcgtacgcgc ttcttttgcc aaacatggcg aaccggatct gctgctgctc tcttatcatg
     8581 gcattcccca gcgttatgca gatgaaggcg atgattaccc gcaacgttgc cgcacaacga
     8641 ctcgcgaact ggcttccgca ctggggatgg caccggaaaa agtgatgatg acctttcagt
     8701 cgcgctttgg ccgggaaccc tggctgatgc cttataccga cgaaacgctg aaaatgctcg
     8761 gagaaaaagg cgtaggtcat atacaggtga tgtgcccggg ctttgctgcg gattgtctgg
     8821 agacgctgga agagattgcc gagcaaaacc gtgaggtctt cctcggtgcc ggcgggaaaa
     8881 aatatgaata tattccagcg cttaatgcca cgccggaaca tatcgaaatg atggctaatc
     8941 ttgttgccgc gtatcgctaa agctgagcgg taaagaactg agcgccgtcg cgaagagcct
     9001 cgtcggcggt tttcatcatt cgtgaataat gcaaaaaggc atgcagcgta cctgggtaga
     9061 gtttgaactc acagggctgc tgatgcgccg ctaacgtctg gtaaagcaga cggctgtcat
     9121 ccagcagcgg atcgaactcc gccccggcaa taaaacaggg cggaacttcg cgagtgagat
     9181 cattattaaa cagacagtaa tacggtgact cgcggtccgc gtcgttgctt aaatacgcct
     9241 cttcatacat ctgcaaatcc tgttgcgtta agccatccca gacaccgccc aacagacgac
     9301 gagtcacgga atcccgtaat ccgtaaagcc cataccacag caaaacaccc gcaattttac
     9361 cgcaatcgat ctgtttatca cgcaaccaca acgcactggc gagcgccagc atggcacctg
     9421 cggaatcacc ggcaaagcca atgcgggaca tattgatttg ataatcctcc gcctgctggt
     9481 ggaaataaca acaagcagcc acaatttcct ctatcgcttg cggaaaacgc gcttcaggtg
     9541 aaagggggta atcaatacca atcaccgtac attggctgta gcttgccagc aggcgcatga
     9601 tgcgatcgtg ggtatcgaga ttgccgagaa taaaaccgcc tccatgcaaa taaaatagcg
     9661 tcgccgggct atctggctgc ggacaaaaga gacgtgtttc cacctgccca tattttgttg
     9721 gaaccatgta agctctggtt gccatttctg gagcgcccgc attccagaat cggcgctcaa
     9781 gcgtgtaata ctgtcgttgc tcagcaatcg ttcccgttgc gggccaggac ggtaaatccg
     9841 actgaagagt attcacaacg gtcttcattt cagcagaaat aaggtccaga acaggtagtt
     9901 tgttttccgg cttcatacaa aaactccttt caaattacgt cattgtaagg aaccactgcc
     9961 atgaaaatgc gatcccgcct gctgatattg aaactggctg cgtctcgcgc gctcccgtca
    10021 gattgtgtta acattcgccg ctcagttaac cacccgtaaa aacaaccatg aaatttcccg
    10081 gtaaacgtaa atccaaacat tacttccctg taaatgcacg cgatccgctg cttcagcaat
    10141 tccagccaga aaacgaaacc agcgccgcct gggtcgtagg tattgaccag acgctggtcg
    10201 atattgaagc gaaagtggat gatgaattca ttgagcgtta tggattaagc gccgggcatt
    10261 cactggtgat tgaggatgac gtagccgaag cgctttatca ggagctaaaa cagaaaaacc
    10321 tgattaccca tcagtttgcg ggtggcacca ttggtaacac catgcacaac tactcggtgc
    10381 tcgcggacga ccgttcggtg ctgctgggcg tcatgtgcag caatattgaa attggtagtt
    10441 atgcctatcg ttacctgtgt aacacttcca gccgtaccga tcttaactat ctacaaggcg
    10501 tggatggccc gattggtcgt tgctttacgc tgattggcga gtccggggaa cgtacctttg
    10561 ctatcagtcc aggccacatg aaccagctgc gggctgaaag cattccggaa gatgtgattg
    10621 ccggagcctc ggcactggtt ctcacctcat atctggtgcg ttgcaagccg ggtgaaccca
    10681 tgccggaagc aaccatgaaa gccattgagt acgcgaagaa atataacgta ccggtggtgc
    10741 tgacgctggg caccaagttt gtcattgccg agaatccgca gtggtggcag caattcctca
    10801 aagaccacgt ttctatcctt gcgatgaacg aagatgaagc cgaagcgttg accggagaaa
    10861 gcgatccgtt gttggcatct gacaaggcgc tggactgggt agatctggtg ctgtgcaccg
    10921 ccgggccaat cggcttgtat atggcgggct ttaccgaaga cgaagcgaaa cgtaaaaccc
    10981 agcatccgct gctgccgggc gctatagcgg aattcaacca gtatgagttt agccgcgcca
    11041 tgcgccacaa ggattgccag aatccgctgc gtgtatattc gcacattgcg ccgtacatgg
    11101 gcgggccgga aaaaatcatg aacactaatg gagcggggga tggcgcattg gcagcgttgc
    11161 tgcatgacat taccgccaac agctaccatc gtagcaacgt accaaactcc agcaaacata
    11221 aattcacctg gttaacttat tcatcgttag cgcaggtgtg taaatatgct aaccgtgtga
    11281 gctatcaggt actgaaccag cattcacctc gtttaacgcg cggcttgccg gagcgtgaag
    11341 acagcctgga agagtcttac tgggatcgtt aagttatcgt cggttcgtag gccagataag
    11401 gcgttcacgc cgcatctggc atttggctct cgatgcctga tgcgacgctg gcgcgtctta
    11461 tcatgcctac atatttttca tattttacat ccggcaacca ccgtttaccc cgtcaccacc
    11521 tcacccgccg gtggcgtttc cagcagttcc agcatggtac gggcgatttc acgctcgccc
    11581 atcactacct gattcgcacc acgttcggtg atatacgcca cttcatcgtc ataatgggcg
    11641 cgggcaataa tctcaatatc cggatttttc acgcgggcag atgccacaat ctcacccgct
    11701 tcataaccgt tgggaatcgt caggatcagc cattttgcac attccagatg cgccagttgc
    11761 ataatttctt cgttcgccgc attgcccaat actgcgcgga ccccgcgctc tcgcagctca
    11821 tcaacacggg ttcgtgacgt ctcaatcacc accagcggaa tatcagaggc gagcaatttc
    11881 tcccccagca ggctgcctac acgaccgtaa cccaccagta gcgcatggtt gcaaatatcc
    11941 actgggatct gcttctcttc ttcgattgcc tcttccagcg tctgctcttc cagcgtttcg
    12001 gtcttcgcca gatatttctc cagtagtgcg aacagtaccg ggttgagcat aatcgacagg
    12061 atcgcccctg ccagtaccag gttttgtccg gcctgcggca gtaaattcaa tgccattccc
    12121 agtcccgcca ggataaacgc gaactcacca atctgcgcca ggctggcggc gatggttaat
    12181 gccgtacgtt gggagtgacc aaacagtcgc accaggaaaa atgcggctaa cgacttacca
    12241 aacagaataa tcgccagcgt cgccagcact gccagcggtt gctgaatcag aattaacgga
    12301 tcaaacaaca tcccgacgga gacaaaaaac agcaccgcaa acgcgtcgcg caatggcagc
    12361 gtatcgtggg cggcacggtg actcagttca gactcgttca gtaccatccc ggcaaagaac
    12421 gcaccgagtg caaaggagac atcaaacagc tctaccgcac caaaggcaat ccctaacgcc
    12481 agcgccagca ccgacagggt aaacagctcg cgagaaccgg ttgccgcgct gcgtgccata
    12541 atccacggca ccagacggcg acctaccagc atcataatgg cgataaatgc gatcactttg
    12601 ccgatggtga tccccatatc gactgcaaga gtggcaaagc ccacatcgcc ctgttccatc
    12661 attcctgcca ctgcgggcag caacaccagc gtcagaacca ttaccaggtc ttccacaatc
    12721 aaccaaccga tggcgatttg cccacgctga ctgtcaatta attgccgttc ttcaagtgcg
    12781 cgcagtaaca ccacggtact ggcggtggaa agacataaac cgaacacgat accggtcatt
    12841 aacgaccagc ccagcacggc ggagagcgcc atacccagca gcgtcgccac ggctatctgg
    12901 gcgatcgcgc cgggaatggc gatggacttt accgccatca aatccttgag cgaaaaatgc
    12961 aaaccgacgc cgaacatcag caaaataacg cccagttcag ccagttccgg cgcaagcttg
    13021 gtatcggcaa caaagcccgg agtgaatggt cctgccagca cacccgctaa cagatatccc
    13081 accagaggag aaatacgtag tttattggcc agcatgccga ggataaaggc gagcacaagg
    13141 ccgccaacaa tggtggtgat aagcggggtg gcgtgatgca ttccgtctcc ttttcctggt
    13201 ggttattgtc catttttggc cgggaaaacc aaaattacag gtaatagttt atgacaattt
    13261 cattgatgat gttcatgaat aattattgaa ttttgcagaa aaatggaatt agctgcaaaa
    13321 aaagcacgga tcggaaaaca gaaggcggtt taacagagga aaggcttatg gcgactgtaa
    13381 aacgatgcag ccaaagtttg cctttggctg caatgaaatc agtctttatg ccggttatca
    13441 ggcaggaata tggtcaacat ccccaatagt ggcaggaaag cacagatttt atagactaac
    13501 tcgatactgg tgtgatcggc gataagcccc agaactgccg ctcccagacc tcccatacca
    13561 aaagcaaaac cgaaaaagag tccagaaacc ataccgatac gtcccggaag cagctcctga
    13621 gcgtaaacca gaatggcaga gaatgccgaa gcgaggataa atccaataat caccgttaaa
    13681 acccccgtcc agtgcaggct ggcgtagggt aaaatcagcg taaacggcgc aacgccgagg
    13741 atagagcccc aaatcacata tttccgccca attttatccc ctacaggccc gccgatcacc
    13801 gtacctgccg caacggcaaa caggaaggca aacagatgaa gctgagcatt ctggatagat
    13861 aatccgaatt tttgcatcag ataaaaggtg taatagctgc tgatgctcgc catatagaaa
    13921 tatttcgaga aaatgaggat taacagaatg ctgaccgcca gtaccacttt gttgcgcggc
    13981 agtggattga taatcgtcgc tttgggtttt cctttattca ttcggtgctg tgccgagtac
    14041 caacggctga tttgcgccaa caccacgatc gccagcagtg ccgcaagcac aaaccaggca
    14101 acgttgcctt tgccataagg cgcgataatc accgccgcca gcaagggtcc cagggaactg
    14161 ccaaagttgc cgccgacctg aaagatagat tgcgccaggc catgccgccc gccggaagcc
    14221 atacgggcta cgcgagaaga ttccggatga aagaccgatg aaccggtacc gaccagcgcc
    14281 gccgccagca gaactgcgcc aaaactgccc gccagcgcaa gcagcaccag accacttaag
    14341 gtaaagcaca tgccaattgg caacgaccac ggcatcggat atttatcggt ccagtagccg
    14401 accactggtt gcagtagcga agaggcgagc tggaaggtga gggttatcat gccaatctgc
    14461 ataaatgtca gagaaaattc tgactgaagc agcggataaa tcgccagaat cagcgattgg
    14521 atcatgtcgt tcagcagatg tgagaggctg atagcaccta aaataccaaa cgatgttcgg
    14581 gccttggtcg ttgacgcagc cgcgcccgcc acaggctggg gttgttcact cattgccata
    14641 ggaaagtcac tttttcaggg ttgcgatgta aagaatgatc ttatttgtga ttattacccg
    14701 actaacatac ctgtatgcgt cgcctgaagg aagtctcaac gccgaataca gaatttctaa
    14761 tctggatgca gatttatctt caccggacgc agacttgtct atgatgtcgc gtcatactat
    14821 ttttcaacac gttgaaatca ggtcagggag agaagtatga aattattgca gcggggcgtg
    14881 gcgttagcgc tgttaaccac atttacactg gcgagtgaaa ctgctctggc gtatgagcag
    14941 gataaaacct acaaaattac agttctgcat accaatgatc atcatgggca tttttggcgc
    15001 aatgaatatg gcgaatatgg tctggcggcg caaaaaacgc tggtggatgg tatccgcaaa
    15061 gaggttgcgg ctgaaggcgg tagcgtgctg ctactttccg gtggcgacat taacactggc
    15121 gtgcccgagt ctgacttaca ggatgccgaa cctgattttc gcggtatgaa tctggtgggc
    15181 tatgacgcga tggcgatcgg taatcatgaa tttgataatc cgctcaccgt attacgccag
    15241 caggaaaagt gggccaagtt cccgttgctt tccgcgaata tctaccagaa aagtactggc
    15301 gagcgcctgt ttaaaccgtg ggcgctgttt aagcgtcagg atctgaaaat tgccgtcatt
    15361 gggctgacaa ccgatgacac agcaaaaatt ggtaacccgg aatacttcac tgatgtcgaa
    15421 tttcgtaagc ccgccgatga agcgaagctg gtgattcagg agctgcaaca gacagaaaag
    15481 ccagacatta ttatcgcggc gacccatatg gggcattacg ataatggtga gcacggctct
    15541 aacgcaccgg gcgatgtgga gatggcacgc gcgctgcctg ccggatcgct ggcgatgatc
    15601 gtcggtggtc actcgcaaga tccggtctgc atggcggcag aaaacaaaaa acaggtcgat
    15661 tacgtgccgg gtacgccatg caagccagat caacaaaacg gcatctggat tgtgcaggcg
    15721 catgagtggg gcaaatacgt gggacgggct gattttgagt ttcgtaatgg cgaaatgaaa
    15781 atggttaact accagctgat tccggtgaac ctgaagaaga aagtgacctg ggaagacggg
    15841 aaaagcgagc gcgtgcttta cactcctgaa atcgctgaaa accagcaaat gatctcgctg
    15901 ttatcaccgt tccagaacaa aggcaaagcg cagctggaag tgaaaatagg cgaaaccaat
    15961 ggtcgtctgg aaggcgatcg tgacaaagtg cgttttgtac agaccaatat ggggcggttg
    16021 attctggcag cccaaatgga tcgcactggt gccgactttg cggtgatgag cggaggcgga
    16081 attcgtgatt ctatcgaagc aggcgatatc agctataaaa acgtgctgaa agtgcagcca
    16141 ttcggcaatg tggtggtgta tgccgacatg accggtaaag aggtgattga ttacctgacc
    16201 gccgtcgcgc agatgaagcc agattcaggt gcctacccgc aatttgccaa cgttagcttt
    16261 gtggcgaaag acggcaaact gaacgacctt aaaatcaaag gcgaaccggt cgatccggcg
    16321 aaaacttacc gtatggcgac attaaacttc aatgccaccg gcggtgatgg atatccgcgc
    16381 cttgataaca aaccgggcta tgtgaatacc ggctttattg atgccgaagt gctgaaagcg
    16441 tatatccaga aaagctcgcc gctggatgtg agtgtttatg aaccgaaagg tgaggtgagc
    16501 tggcagtaat ccgaaagtgc cggatgtttg catccggcac aatgcttaat cgcggcgggc
    16561 gatatcagca aatttggcat cgaggatctt tgccagatcg cctgccgcca gttcgatatc
    16621 cagtccgcgc ttgccgccgg aaacataaat agtggcaaat tcttgtgcgg gggcgtcgat
    16681 aatcgttggc agacgttttt tctgccccag tgggctaatc cccccaacca ggtatcccgt
    16741 cgaacgctgc gcgaccatcg gatcggccat ctcaactttc ttggcaccca gcgcttttgc
    16801 tactttttta agatccagtt gacctgcgac cggcgtaacg gccacggcaa ggtgtttcat
    16861 atcaccgttc actgccacca gcagcgtttt gtagacctga tccggattca aacctaattt
    16921 tttgacgact tcatcgccaa aattggtttc agccggatcg tgctcgtagg tatggatttg
    16981 aaacgaaatc ttgttttttt cgagtaattt aactgcgggt gtcatggtaa ttttcctgcc
    17041 ttaaacaaaa aaacgaagca agcatacgcc tgatggcggt ccaaaaaaaa gagtcatctt
    17101 gcctaagagt attggcagga tggtgagatt gagcgacaat cgagttacac cgtcgcttaa
    17161 agtgacggca taataataaa aaaatgaaat tcctctttga cgggccaata gcgatattgg
    17221 ccattttttt agcgcaacat ttgcggcaaa ttcccttctc catacaggtg tagtgcaccg
    17281 accgcgacca catatcgccc cggcggcatg gcgcgtaatt tatcccgcca ggcgagattt
    17341 cgctgatgca tcagcacatc gtacagcgac tgactgaacg tattgggcag cgttatttca
    17401 ttattttgcg gcggcgcatt cagccaccag ctcatcattt gttgcagcaa ccgtgcgttg
    17461 gtatgccagt gggtcagcgt atcgtccagc agcgccagtc ctttgtcagg gagctggagc
    17521 aacatggcaa tctggttttc agccccttcc agttcaatca cgggtttatg ttgttgcttc
    17581 gccgcctgca atagctggta atcgataccg tattctgccc gcagccccag tttttgtgcc
    17641 tgcgtcgcct gaagaaccat cgcgatttgc cacagcggtt gggtagaaaa gagtgaggga
    17701 gaaatgccca tctcctggct aatgtgctgc agattttgga gttgctcctc gctaatgcgc
    17761 tcttccagcg cctcgcaggc aggcaaatta gcaaaaggcg tatcgctggt ggaaacatcc
    17821 gcctcgacga tcagcgcatc ggcgtttttg agctttttga gcaaacgggt gggcagggga
    17881 gccatatcgt ggctacccat atgaatactg ccaatcagat gaaaatggcg attgccgggg
    17941 agggtgatat cgatggcagg ccaggtgtaa tgattaccgc gcagcgcggc ccaaagcgtt
    18001 tttacccggt acaacagatc catacgacct ccctttgtga aatatcatgc tagcgcgcgg
    18061 tgagggatgg cgcaaatgga gttggggact tgtttaatgt gtttgtatga ttcagtatgt
    18121 tcttgcatcg ctattcacag ggaagcaaca gttaaaaacc atgaaacagg caacaagaaa
    18181 accgacgaca cccggagata ttcttctcta tgaatatctg gaaccgctcg atttgaaaat
    18241 caatgagtta gcagagttgc tgcatgttca tcgtaatagc gtcagtgcac tgatcaataa
    18301 caatcgtaaa ctcaccactg agatggcatt ccgtctggcg aaagtttttg ataccacagt
    18361 cgattttagg ctaaacctcc aggcggcagt tgatctttgg gaagttgaaa acaacatgcg
    18421 cacccaggaa gaattgggac ggattgaaac agtggctgaa tatttggcac gccgtgaaga
    18481 gcgtgcaaaa aaggtcgcgt aaggccaaaa catcgcttca tctcgtaggc cggataaggc
    18541 gtttacgccg catccggcag tcatgcgtcg atgccaaatg cgccgcccta aagcagcgca
    18601 tccgcaatga cgtacttatt ccttcggttt aaaccgcagc aaccggttgg cgttactcac
    18661 tacggtaatc gacgagagcg ccattgccgc tccggcaact accgggttaa gcagtgttcc
    18721 agtaaacggc cacaaaatac cggcggcgac cggaataccg atgctgttgt agataaaggc
    18781 accgagcagg ttctgcttca tgttgcgcag cgttgcgcgg gaaatcgcga gcgcatccgc
    18841 aacgcccatc aggctatggc gcatcagggt aatcgccgcg gtttcaatgg caacatcact
    18901 gccgccaccc atcgcaatgc cgacatccgc ctgggccagc gctggcgcgt cgttaatgcc
    18961 gtcgcccacc attgccacct gacgtccttc actttgcaga cgtttgatcg cttcggcttt
    19021 accgtctggc agcactccgg caatcacttc atcaattcca gcttctttgg cgatcgcatt
    19081 ggcggtggtt gggttatccc cggtcaacat cagcagacga tatcccgctt tatgcaggcg
    19141 ttgcagcgcc gccacgctat cactacgcaa cggatcgcgt acagccagca gggctactgc
    19201 tttcccgtca atcgccagca gcacaggcgt tgctccttgc gatgcctgag cagtaatctc
    19261 cgcttcgata gctttggtac caacctgttg ctcatttaac agcgcctgat tgcccagcaa
    19321 taacgcatga ccttcagctt caccgctcac gcccagcccg cgcaatgtgc ggaaaccgtt
    19381 gacctgcggc agctgcatat cacctgcttt atcgaggatc gcccgtgcca gcggatggct
    19441 ggaaccttgc tccagtgccg ccgccagacg caatgcctgc gcttcatcaa catcagcaaa
    19501 cgttttcact gcgacaacct gcggcttccc ttcggtcagc gtcccggttt tatcgaacac
    19561 gacggtatcg agtgtactgg cgcgttgcag cgcgtcggcg tcccgcacca gcacgccaaa
    19621 ctcagccgcc cgcccgacgc cggaaataat cgacatcggc gtcgccagcc ccagcgcaca
    19681 cggacaggcg ataatcagta ccgtggtggc aatcaccagg gtataaacaa tttgcggtgc
    19741 cggaccaaag aagtaccaga ttgccgcact gaccagcgca atcaccacca ccacaggtac
    19801 aaatacggct gagattttat ccgccagctg accgatttct ggcttgctgc tctgggcctg
    19861 gcgcaccatg cgaatgatcc gtgacagcgt ggtatggctg ccaaccgcac tggcacgaaa
    19921 cagcacgcta ccgtcctgta ccactgtccc ggcatggacg ctttcgcctt cgcctttttg
    19981 ctgcgggatt ggttcgcccg tcagcatcgc ttcatccagc catgcttcgc cctgggtaat
    20041 ctcgccatct accggcacgc gatcgccggt cgtcaggcgc agcaacatac ctggctgcac
    20101 ttccgccaga ggcacgcttt tttcaccttc gtcagtaacc aggcgcgccg tcggtggggt
    20161 taaatcgagt aatttctcca gcgccttaga agaacgctga cgtgcgcgtg cttccagcat
    20221 atggccgaga ttgatcagac cgataatcat cgcgctggct tcgtaataaa gatgtcgcgc
    20281 ttccatcggg aaccactgcg gccacaggtt gacgctcatc gaatagagcc acgccacgcc
    20341 agtacccagc gccaccagcg tatccatcgt cgctgcaccg ttcagcaggc ttttccatgc
    20401 actgcggtaa aaatggccgc cggcgaaaac catcaccgcc agggttatca ggccgataac
    20461 caaccacagg ctgcggttgt cagcggtgac catcatgtta tcgccaatca tcccccagac
    20521 catcaccggg atacccaccg ccagtgcgac aattgcctgc cagcggaagc gcttcatcgt
    20581 agcaacggcg gtttcctgct ggcgctcacg gcgtttcgcg tcatcttcaa tcgcttccgc
    20641 gccgtagccc gctttttcca ccgcctgcac taaatcttgt ggggaggcac tgcccatcac
    20701 cagcgcagta cgctccgcca ggtttacccg tgcctgagtg acgcccggta cgctttgcag
    20761 cgcattttgt acgcgggtga cacagctggc gcagctcatg ccgctcagca gcaactgctg
    20821 gctgtcatcg tcatcggcgg tcgctgccgg aagcgcctca gaaaccgctg tcagtgcttc
    20881 cgacgggatt gatgactccg ccagcggttt agcctttggg tggcttacag atgcgtcata
    20941 acccgcttgt ttgatggttt cgatcagctg ttctgcactg gcagtcccgg taacgtgcgc
    21001 ttcagtgata gacacatccg cttgttccac atccgggcgc tgttcgagac tttctttcac
    21061 gcgtttaacg cagtgaccgc aggacaggcc gtccagggtc aggtcgatag tttgtgacat
    21121 aaaacactcc tttaagacag ttttgactgg ctgtgataaa ggttaaacct tccagcaagg
    21181 ggaaggtcaa gaaattaata aaccaggcga gtaaaagtcc gtaaagatta aaaaattggc
    21241 tcaatttgca tcaggattag acatttatct ctttgttttc ctgaagttaa gttgcgggtg
    21301 ctaagttaaa tcaagatgcc tgaaaatcgg caccggggtg aggaattacc tcccgcatct
    21361 ataaaaagga gttaacaaaa gatgttagat gcaaacaaat tacagcaggc agtggatcag
    21421 gcttacaccc aatttcactc acttaacggc ggacaaaatg ccgactacat tccctttctg
    21481 gcgaatgtac ctagtcaact ggcagcagtg gctattgtga cctgcgatgg caacatctat
    21541 cgcgcgggtg atagtgatta ccgctttgcg ctggaatcca tctcgaaagt ctgtacgtta
    21601 gcccttgcgt tagaagatgt cggcccgcag gcggtacagg acaaaattgg cgctgacccg
    21661 accggattgc cctttaactc agttatcgcc ttagagttgc atggcggcaa accactgtcg
    21721 ccactggtaa atgctggcgc tattgccacc accagcctga ttaacgctga aaatattgaa
    21781 caacgctggc agcgaatttt acatatccaa cagcaactgg ctggtgagca ggtagcgctc
    21841 tctgatgaag tcaaccagtc ggaacaaaca accaacttcc ataaccgggc cattgcctgg
    21901 ctgctgtact ccgccggata tctctattgt gatgcaatgg aagcctgtga cgtgtacacc
    21961 cgtcagtgct ccacgctcat caatactgtt gaactggcaa cgcttggcgc gacgctggcg
    22021 gcgggtggtg tgaatccgct gacgcataaa cgcgttcttc aggccgacaa cgtgccgtac
    22081 attctggccg aaatgatgat ggaagggctg tatggtcgct ccggtgactg ggcgtatcgc
    22141 gttggtttac cgggcaaaag cggtgtaggt ggcggtattc tggcggtcgt ccctggcgtg
    22201 atgggaattg ccgcgttctc accaccgctg gacgaagaag gcaacagtgt tcgcggtcaa
    22261 aaaatggtgg catcggtcgc taagcaactc ggctataacg tgtttaaggg ctgatcatga
    22321 tgaacacgga aggtaataac ggtaacaaac ctctcggtct atggaacgtc gtttccatcg
    22381 gcattggggc aatggtgggg gcggggatct tcgcgctgct ggggcaggct gcattgctaa
    22441 tggaagcctc gacctgggtc gcctttgctt ttggcggtat tgtggcgatg ttttccggtt
    22501 atgcctatgc gcgtctgggg gcgagctatc ccagcaatgg cggcattatc gacttctttc
    22561 gtcgcggatt aggcaacggc gtcttttcgc tggcgctctc gttactgtac ctgttgacgc
    22621 tggcggtgag catcgccatg gtcgcccgtg cttttggcgc ttatgccgtg cagtttttgc
    22681 atgaaggcag ccaggaggag caccttattt tgctctacgc gttggggatc attgcggtga
    22741 tgacgctttt caactcctta agcaaccatg cggtagggcg gctggaagtg atcctcgtcg
    22801 gcattaaaat gatgatcctg ttattgctga ttattgccgg tgtctggtcg ctgcaaccgg
    22861 cgcatatttc cgtctctgcg ccccccagct ccggtgcgtt cttctcctgt atcgggataa
    22921 ccttccttgc ctatgcgggc tttggcatga tggcgaacgc ggcggataaa gtgaaagatc
    22981 cgcaggtcat tatgccgcgg gcgtttctgg tggcgattgg cgttaccacg ttgctttata
    23041 tctcgctggc actggttttg cttagcgatg tatcggcatt agagttagaa aaatatgccg
    23101 ataccgccgt agcgcaggct gcttctccgc tgctcggaca tgtgggttat gtgatcgtcg
    23161 tcatcggcgc tttactggcg acggcttcag ccattaacgc gaacctgttc gccgtgttta
    23221 acatcatgga caacatgggc agcgaacgcg aactgccgaa gctaatgaat aaatccctgt
    23281 ggcggcagag tacctggggc aacatcattg tcgtggtgtt gattatgctg atgacggcgg
    23341 cgctaaattt aggctcactc gccagtgttg ccagcgccac ctttttgatt tgctacctgg
    23401 cggtgtttgt ggtggcgatc cgcctgcgtc atgatattca cgcctcgttg ccgattctta
    23461 tcgttggtac gttggtgatg ttgttggtga tcgttggctt tatctacagc ctgtggtccc
    23521 agggtagccg tgcgttgata tggattattg gctcactctt actcagcctt attgtggcaa
    23581 tggtcatgaa gcgcaataaa accgtataac atctctctgt gcgcagtact tcctgtatta
    23641 ttgtggtggc ggtcgatatt cgcactggca aaaaaacgtg cttgaatatc tgttgaaacc
    23701 ctttaacaaa gcacaggagg cgttgcgcga acgatgaaca tcagcgatgt agcaaaaatt
    23761 accggcctga ccagcaaagc cattcgcttc tatgaagaga aggggctggt gacgccgccg
    23821 atgcgcagcg aaaacggtta tcgcacctac acgcagcagc atctcaacga actgacctta
    23881 ctgcgccagg cacggcaggt gggctttaac ctggaagaga gcggcgagct ggtgaatctg
    23941 tttaacgacc cgcagcggca cagcgccgac gtcaaacggc gcacgctgga gaaggtggcg
    24001 gagatcgaac gacacattga ggagctgcaa tccatgcgcg accagctgct ggcactggcg
    24061 aatgcctgcc ctggcgatga cagcgccgac tgcccgatta tcgaaaatct ctccggctgc
    24121 tgtcatcatc gggcagggtg attaagacga gacggctcgg atatgtaggg ttatcccttc
    24181 tatcgcaatg acttcaacat gcgtacctgc accgagatcc tcgctggcgc tgacaggcca
    24241 tgaactgtca ccgacgcgca tatgaccgcg cccgttgacc agcggagatt ccagcacaaa
    24301 acgtcggcca atcagctgct gcccgcgctg gtttaaatga ctatcgctgt gcttttgttc
    24361 gcgcacccgc cgcgacaacc atttccacca cagccaggcg gcgagcagcg tcaggatggc
    24421 aaacatcacc ccttgccact cccaacccag cggcaccagc cagaccacca ggccggtaat
    24481 caccgctgac acgccgctcc acagcaaata accatttccg cccagcatct cggctgccag
    24541 cagcaacccg ccgagactga gccagaaaat atgtggatga acgaccatta actccatcat
    24601 ggctgagtcc gtttgttggc gctgtctttc accagctcgg caatcccggc aatcgacccc
    24661 atcaggctgc tggcctctaa tggcatcatc actactttgc tgttactgga ggaaccgatc
    24721 tgctgtaacg cttcggtgta tttctgcgct acgaagtagt tcaccgcctg aatatcaccg
    24781 gaggcaatgg cttcagatac cattttggtg gcgcgggctt ctgcttcggc agaacgttca
    24841 cgcgcttcgg cctgtaagaa tgccgactga cgttcacctt ccgctttcag aatttgcgac
    24901 tgtttttcac cttccgcttt gagtatttcc gcctgacgga tcccttccgc ttcaaggatg
    24961 taagcgcgtt tggtacgttc cgctttcatc tgcgcgttca ttgaagagat aagctctgcc
    25021 ggtgggcgca cgtcgcgaat ttcaatacgg gtgactttaa tcccccacgg gttggtggct
    25081 tcatcgacaa tacgcagcag gcgtgagttg atgctgtcgc gctgagagag catttcgtca
    25141 agttccattg aacccaacac ggtacggatg ttagtcatgg tcaggttgat gatcgccagc
    25201 tccagattgc tgacttcata agccgcgcgc ggcgcgtcaa tcacctgaat aaaacagact
    25261 gcgtcgatgg taacgttggc gttatctttc gagataactt cctgggaagg gatatcgagc
    25321 acttgctcca tcatattgat cttgcgacca atgcgatcca taaacggcac caccagactg
    25381 agccccggct gtaacgtttt ggtatagcga ccaaaacgtt ctaccgtcca ttgatagccc
    25441 tgcggcacga ttttgacgcc cgcgccgaca atgaccagcg cgacaaaaat gagaatcggg
    25501 ataaagataa gcatcggaaa aacctcctgt tgtaccgtcc ataatcagca aaattgctgc
    25561 ttgattaaac aaattatacc tgattactga aagagagttc ccccttattc ctgcgaagga
    25621 taaactgttt ttagtaaaaa tcagaaaaag ggaacagtga tgcaggaaaa tagtcctttg
    25681 cttcagctac aaaacgtagg atatctggcg ggtgatgcga agattcttaa taacatcaat
    25741 ttttcgctgc gtgctggcga atttaagtta attaccggtc cttccggttg tggcaaaagt
    25801 acgctgctaa aaatagttgc ttcattgatc agcccaacca gcggaacgtt actgtttgaa
    25861 ggtgaggatg tcagcacact aaagccagaa atctaccgcc agcaagtctc ttactgcgcc
    25921 cagacgccga cgctgtttgg cgatacggta tacgataatc tgatctttcc ctggcagatc
    25981 cgtaaccagc agcctgaccc ggccattttt ctcgattttc tcgaacgctt cgctttgccg
    26041 gacagcattt tgacgaagaa tatcgccgag ctatctggtg gtgaaaaaca acgcatctca
    26101 ttgattcgta acctgcaatt tatgccgaag gttttattgc tggatgaaat aaccagtgcg
    26161 ctggatgaaa gtaataaaca taacgtcaat gagatgatcc atcgttatgt gcgcgagcaa
    26221 aatattgccg tgctgtgggt gacacacgat aaagacgaaa ttaatcatgc ggataaagtg
    26281 attacactgc aaccgcatgc cggagaaatg caggaagcac gctatgaact cgcataatat
    26341 tactaacgaa tcattagcac tggcattaat gctggtggtg gtggcaatct taattagcca
    26401 taaagaaaaa ctggcgctgg agaaagatat tctctggagc gtcgggcgag cgataattca
    26461 gctgattatt gtcggctatg tgctgaagta tattttcagc gtggatgatg ccagcctgac
    26521 attattgatg gtgttattta tctgctttaa tgcggcgtgg aacgcgcaaa aacgcagtaa
    26581 atatattgct aaagctttta tctcatcgtt tattgccatt acggtcgggg cgggaattac
    26641 cctggcggtg ctgattctct cagggtcgat tgaatttatc ccgatgcagg tgatccctat
    26701 cgccgggatg attgccggta acgccatggt agcggtgggg ttgtgttaca acaatttagg
    26761 gcaacgggtc attagcgaac agcaacagat tcaggagaaa ctgagtcttg gtgcgacgcc
    26821 gaagcaggct tcagcgatat tgattcgcga cagtattcgc gcggctttaa ttccgacggt
    26881 cgattcagca aaaacggttg gcttagtgag tttaccagga atgatgtccg ggctgatatt
    26941 tgccgggatt gatccggtga aggcgattaa atatcagatt atggtgacct ttatgctgct
    27001 ctcaaccgcc agcttgtcga ccattattgc ctgctattta acctatcgta agttttataa
    27061 ttcgcgccac cagttggtgg tgacgcaatt gaagaagaaa tgatgcgatg ccggatgcgc
    27121 agcatcgcat ccggcgttgt ggttcatgtg ccgatcaacg gaatatcaat acaacaatgc
    27181 atacagctgg cggcgatact tcgacgccag tgcatcaccc gtacccagcg cagcgaggat
    27241 ctcctggaac gttttacgcg tctgaccgtc tgcggcggtg agatctttac gcagatgccc
    27301 gaacagcaac tccagcgcct cttcattgcg cccaacctga tgcagttgca gcgccagttg
    27361 cgtcgccagt gcggcatctt ctggattctc cgccacctgc tgttgcaact gttgaatttc
    27421 cggagtatca gccgcctgct tcagcagttc gatttgcgcc accagcccct ggtagcgggt
    27481 gtcctgatcc tgcaacggaa tggttttcag caccgcttcc gcatcttcag aacggttcag
    27541 cgcaatcagc gtttctgcca gcagcaggcc gatctccccg ttctgattcg acaactgcca
    27601 ggcgtctttc agcaatggca gggcatcggt gtaattgctt tcctgcatca gttgcatcgc
    27661 ctgctgcgct ttcagctctt cttcgcgcgg cagcacttta tccagcaggg cgcggatcgc
    27721 ctcttccggt tgcggcccct ggaagccatc taccggttgc ccgttctgga acagatacac
    27781 ggtcggaatc gcgcgcagac caaactgggc ggcaatcatc tgctccgcgt cgcagtccag
    27841 cttcgccaga ataaattgcc cgttgtactg cgccgcgagg ctttccagga tcggggttaa
    27901 ctgcaaacag tgctggctac gttcagacca aaaatagaac agcaccggag tggtcatcga
    27961 ctgttcaaga acctgttgca ggttagattc gttaatgttg acaatatttt ctacggacat
    28021 ggagtcgctc tctgttgtcg atattttctt tgacatgggg gcttaagcgc gcgcttcaac
    28081 tcacccctgc aatattttgt ccatcacgcg ccccggcagc aggcgcttaa gcaccattac
    28141 tgcccaggtc accagcgtta ccggatagcg catcttcggc ttctcgctaa taaaagcatg
    28201 gcgtactttg tccaccaccg cttccggtcc caacgtaaag cgggcggcga tgccgggatt
    28261 ttcgactggt ttatcacttt gcgtctggtt gacgttgtcg gtgaagcgag tacgaatggg
    28321 accgggttcg atcaggctga ctttaattcc gctgtggcgt agctccatgc gcagcgcgtc
    28381 tgaccacgcc tccagcgcat atttactggc cgcgtaagcg ccacgacccg gcgtggagat
    28441 taatcccatc accgatgatg tcatcacaat acgcccttca ccgtgcggta acatcgcggg
    28501 taacaggcgc atggtgagct ggtgtgcgcc gaaaaagttg gcggaaaact gctgttccat
    28561 ctgcgcacgg ctgatggtgg aaagggggcc atacatgccg aatccggcat tgttaaagat
    28621 cccatacaga caattatcgg tcagggcgat cacctcgtct gctgcgcgat caacactttc
    28681 tggtgaatcc agatcgatca acacgccggt aaatcccatg ttgttcatac gctcaacatc
    28741 atccggtttt cggcaacctg ccagcacatg aaaaccctgg cgttttaatt cgagcgcgct
    28801 ttccaggcca attccactgg aacatccggt aattaagacc gatttttgca taactttacc
    28861 tgtcaggatc tccgttgctt tatgagtcat gatttactaa aggctgcaac tgcttcgcca
    28921 tccagtcggc aataaacggc tgggcgtcgc ggttgggatg aataccgtca tcctgcatcc
    28981 attgtggctt gaggtagacc tcttccataa aaaagggcag cagcggaaca tcaaactctt
    29041 tggcgagttt ggggtaaatg gcgctaaagg cttcattata acggcgacca tagtttgcag
    29101 gcagacgtat ttgcattaac aatggttcag cgttggcggc tttgacatcc tgcaaaatct
    29161 ggcgcagcgt ttgctcggtt tgctgtggct gaaaaccacg caaaccgtca ttgccgccca
    29221 gttcaaccag cacccagcgc ggctgatgct gtttcagcag agccggaagg cgcgccagtc
    29281 cttgttgcga ggtgtcgccg ctgatgctgg cattaactac cgacgtttta ctctgccact
    29341 tatcattcaa caaggcaggc caggccgcgc tggcagacat tcgatacccg gcgctcaggc
    29401 tatcacccag aatcaataac gtgtccgctg cggcggcacg gaaggttaac aggaccagga
    29461 acaggaaggg caaatgccag cggaaaacat tgttgaagtt catcatctta agaagtccgt
    29521 cggtcagggg gagcatgaac tctccatcct caccggagtt gagctggttg tcaaacgtgg
    29581 cgagaccatc gcactggtgg gcgagtcggg atcgggtaag tcaaccttgc tggcgatcct
    29641 cgccgggctt gatgacggca gcagtggcga agtgagtctg gtgggacaac cgctacataa
    29701 tatggacgaa gaagcgcggg caaagttgcg cgcgaagcac gtcggctttg tttttcagtc
    29761 atttatgtta attcctaccc ttaacgcgct ggaaaacgtc gagcttccgg ctctgctgcg
    29821 cggtgagagt agcgcggaaa gtcgtaacgg ggcgaaagcg ttgctcgaac agttagggct
    29881 gggtaaacgt ctggatcatc ttccggcaca gctttccggc ggtgaacagc aacgagtggc
    29941 gctggcacga gcctttaatg gtcgacctga tgtgctgttt gccgacgaac ccaccggcaa
    30001 ccttgaccgc cagacgggcg ataaaattgc cgacctgctg ttttccctca accgtgaaca
    30061 tggcaccacg ttgattatgg tgacccacga cctgcaactg gcggcacgct gcgaccgctg
    30121 cttacggctg gtgaacgggc agttgcagga ggaagcatga ttgcacgttg gttctggcgc
    30181 gaatggcgtt cgccgtcgct attaattgtc tggctggcgc taagcctggc ggtggcctgc
    30241 gtgctggcgc tgggcaatat cagcgatcgc atggagaagg gcttaagcca acaaagccgg
    30301 gagtttatgg cgggcgatcg ggcgttgcgc agttcgcgcg aagtgccgca agcatggctg
    30361 gaggaagcgc aaaagcgcgg cctgaaagtc ggcaagcagc tgactttcgc caccatgaca
    30421 tttgcaggcg acacaccgca gctggcgaac gtcaaagcgg tggatgatat ctacccgatg
    30481 tatggcgatc tgcaaactaa tccccctggc ctgaaaccgc aggcgggcag cgtattgctg
    30541 gccccacgcc tgatggcgct gcttaacctg aaaacgggcg acaccattga cgtgggcgat
    30601 gccaccttgc ggattgccgg agaagtgatt caggaaccgg attccggttt taaccccttc
    30661 cagatggctc cgcgtctgat gatgaatctg gcggatgtcg ataaaaccgg agccgtgcag
    30721 ccggggagtc gggtcacctg gcgttataaa ttcggcggca acgagaacca gctcgacggc
    30781 tatgagaaat ggttgttacc tcagcttaaa cccgaacaac gctggtacgg tctggaacag
    30841 gacgaaggcg cgctggggcg atcgatggaa cgctcgcaac agttcctgct gctttcggcg
    30901 cttctgacct tgctgctggc agtggcagcg gtggcggtag cgatgaatca ttactgtcgc
    30961 agtcgctacg atctggtggc gatcctcaaa acgctggggg cagggcgagc gcaactgcgt
    31021 aagcaaatcg tcggtcagtg gttgatggtg ctggcgcttt cagccgttac cggtggggcg
    31081 ataggcctgt tgttcgaaaa cgtgttgatg gtgctgctca agcccgttct gcctgctgca
    31141 ctaccgccag ccagcctctg gccgtggctg tgggcgctgg gtaccatgac ggtcatctcg
    31201 ctgctggtgg ggctacgacc gtaccgcctg ttgctggcaa cgcagccttt acgcgtatta
    31261 cgtaatgatg tggtagcgaa cgtctggccg ctgaagtttt atctgccgat tgtcagtgtg
    31321 gtggttgtgc tgctgctcgc cggattaatg ggtggcagca tgctgctttg ggcggtgctg
    31381 gcgggcgcgg tagtactggc tttgctgtgc ggtgtgctgg gctggatgct gctgaatgta
    31441 cttcgccgca tgacgctgaa atcgctacct ctgcgcctgg cggttagccg cctgttacgt
    31501 cagccgtggt caacattaag ccagctttcg gcattttcgc tctcctttat gctgctggcg
    31561 ctgttgctgg tattgcgtgg cgatctgctt gaccgctggc agcagcagct acctccagaa
    31621 agcccgaact actttttaat taacattgcc acagaacagg tagaaccgct aaaagcgttc
    31681 ctcgcggaac atcagatagt cccggaatca ttttatccgg tagtgcgggc gcggctgacg
    31741 gcgattaacg ataagccgac agaaggtaat gaagatgagg cgcttaatcg cgaactcaat
    31801 cttacctggc aaaatacgcg gcccgatcat aatccgattg tcgccggtaa ctggccgcca
    31861 aaagccgatg aagtgtcgat ggaagagggg ctggcgaaac gcttaaacgt tgccctcggt
    31921 gataccgtga cttttatggg cgatacccag gagttccgcg ctaaagtgac cagcctgcgc
    31981 aaagtggact gggaaagtct gcggcctaat ttctatttta ttttcccgga aggggcatta
    32041 gacgggcaac cgcagagctg gcttaccagt ttccgctggg agaatggcaa cggcatgttg
    32101 acgcaactca accgccagtt cccgaccatt agcctgttag atattggcgc gattttaaaa
    32161 caggtcggtc aggtgctgga gcaggtaagt cgggcgctgg aggtgatggt ggtgctggtc
    32221 accgcctgcg gtatgttgct gttgctggca caggtgcagg tgggaatgcg ccagcgtcat
    32281 caggagctgg tggtatggcg cacactcggt gcggggaaaa aactgctgcg cacgacattg
    32341 tggtgtgagt tcgccatgct tgggtttgtt tccggcctgg tggccgcaat tggtgcggaa
    32401 acggcactgg cagtgttgca ggcgaaagtg tttgatttcc cgtgggagcc agactggcga
    32461 ttgtggattg ttctgccgtg cagcggtgcg ctgctgctgt cgcttttcgg cggctggctg
    32521 ggtgcgcgac tggttaaggg taaggcgctg ttcaggcagt ttgcggggtg atgagagtaa
    32581 ataaggggcg ttctggttga atggaacgcc ttgtttaaat taat
//
LOCUS       DAZGVQ010000039        32384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000039 DAZGVQ010000000
VERSION     DAZGVQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.039"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            68..1357
                     /gene="hpaG"
                     /locus_tag="ACHLAG_001799"
     CDS             68..1357
                     /gene="hpaG"
                     /locus_tag="ACHLAG_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFP0655044.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKIREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            1354..2820
                     /gene="hpaE"
                     /locus_tag="ACHLAG_001800"
     CDS             1354..2820
                     /gene="hpaE"
                     /locus_tag="ACHLAG_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFP0655045.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2822..3673
                     /gene="hpaD"
                     /locus_tag="ACHLAG_001801"
     CDS             2822..3673
                     /gene="hpaD"
                     /locus_tag="ACHLAG_001801"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFP0655046.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3683..4063
                     /locus_tag="ACHLAG_001802"
     CDS             3683..4063
                     /locus_tag="ACHLAG_001802"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFP0655047.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4174..4977
                     /gene="hpaH"
                     /locus_tag="ACHLAG_001803"
     CDS             4174..4977
                     /gene="hpaH"
                     /locus_tag="ACHLAG_001803"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFP0655048.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4988..5776
                     /gene="hpaI"
                     /locus_tag="ACHLAG_001804"
     CDS             4988..5776
                     /gene="hpaI"
                     /locus_tag="ACHLAG_001804"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFP0655049.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            5899..7275
                     /gene="hpaX"
                     /locus_tag="ACHLAG_001805"
     CDS             5899..7275
                     /gene="hpaX"
                     /locus_tag="ACHLAG_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFP0655050.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSLVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMIYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVATLLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7285..8175
                     /gene="hpaA"
                     /locus_tag="ACHLAG_001806"
     CDS             7285..8175
                     /gene="hpaA"
                     /locus_tag="ACHLAG_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFP0655051.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8426..9988
                     /gene="hpaB"
                     /locus_tag="ACHLAG_001807"
     CDS             8426..9988
                     /gene="hpaB"
                     /locus_tag="ACHLAG_001807"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFP0655052.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGSMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10006..10518
                     /locus_tag="ACHLAG_001808"
     CDS             10006..10518
                     /locus_tag="ACHLAG_001808"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFP0655053.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10911..13061
                     /gene="btsT"
                     /locus_tag="ACHLAG_001809"
     CDS             10911..13061
                     /gene="btsT"
                     /locus_tag="ACHLAG_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFP0655054.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLNVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13111..13314
                     /gene="yjiX"
                     /locus_tag="ACHLAG_001810"
     CDS             13111..13314
                     /gene="yjiX"
                     /locus_tag="ACHLAG_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP0655055.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13325..14281
                     /gene="yjiA"
                     /locus_tag="ACHLAG_001811"
     CDS             13325..14281
                     /gene="yjiA"
                     /locus_tag="ACHLAG_001811"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFP0655056.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(14359..16899)
                     /locus_tag="ACHLAG_001812"
     CDS             complement(14359..16899)
                     /locus_tag="ACHLAG_001812"
                     /EC_number="3.5.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008953 - penicillin amidase activity
                     [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin G acylase"
                     /protein_id="HFP0655057.1"
                     /translation="MKNRNRMIVNCVTASLMYYWSLPALAEQSSSEIKIVRDEYGMPH
                     IYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPD
                     AIRAQIAALSPEDMSILQGYADGMNAWIDKVNTNPETLLPKQFNTFGFTPKRWEPFDV
                     AMIFVGTMANRFSDSTSEIDNLALLTALKDKYGVSQGMAVFNQLKWLVNPSAPTTIAV
                     QESNYPLKFNQQNSQTAALLPRYDLPAPMLDRPAKGADGALLALTAGKNRETIAAQFA
                     QGGANGLAGYPTTSNMWVIGKSKAQDAKAIMVNGPQFGWYAPAYTYGIGLHGAGYDVT
                     GNTPFAYPGLVFGHNGVISWGSTAGFGDDVDIFAERLSAEKPGYYLHNGKWVKMLSRE
                     ETITVKNGQAETFTVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAK
                     NWQEWTQQAAKQALTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKG
                     LLPFEMNPKVYNPQSGYIANWNNSPQKDYPASDLFAFLWGGADRVTEIDRLLEQKPRL
                     TADQAWDVIRQTSRQDLNLRLFLPTLQAATSGLTQSDPRRQLVETLTRWDGINLLNDD
                     GKTWQQPGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTGSLNISVGA
                     KILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVSNWKTPAMA
                     LTFRANNFFGVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWDVVAPGQSG
                     FIAPDGTVDKHYEDQLKMYENFGRKSLWLTKQDVEAHKESQEVLHVQR"
     gene            complement(17251..17415)
                     /gene="yjiS"
                     /locus_tag="ACHLAG_001813"
     CDS             complement(17251..17415)
                     /gene="yjiS"
                     /locus_tag="ACHLAG_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFP0655058.1"
                     /translation="MEFHENKAKTPFIRLVQLWHAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            17592..19004
                     /gene="yjiR"
                     /locus_tag="ACHLAG_001814"
     CDS             17592..19004
                     /gene="yjiR"
                     /locus_tag="ACHLAG_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP0655059.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMRLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DNSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(19246..20190)
                     /locus_tag="ACHLAG_001815"
     CDS             complement(19246..20190)
                     /locus_tag="ACHLAG_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP0655060.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            20657..21889
                     /gene="mdtM"
                     /locus_tag="ACHLAG_001816"
     CDS             20657..21889
                     /gene="mdtM"
                     /locus_tag="ACHLAG_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFP0655061.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            21930..23210
                     /gene="yjiN"
                     /locus_tag="ACHLAG_001817"
     CDS             21930..23210
                     /gene="yjiN"
                     /locus_tag="ACHLAG_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFP0655062.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPVLLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLSNF"
     gene            23326..24477
                     /gene="yjiM"
                     /locus_tag="ACHLAG_001818"
     CDS             23326..24477
                     /gene="yjiM"
                     /locus_tag="ACHLAG_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFP0655063.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLALRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            24487..25254
                     /gene="yjiL"
                     /locus_tag="ACHLAG_001819"
     CDS             24487..25254
                     /gene="yjiL"
                     /locus_tag="ACHLAG_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFP0655064.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            25251..25508
                     /gene="ytiA"
                     /locus_tag="ACHLAG_001820"
     CDS             25251..25508
                     /gene="ytiA"
                     /locus_tag="ACHLAG_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFP0655065.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTESIYCQQDGVWRCIAHYGVSPR"
     gene            25573..26433
                     /gene="yjiK"
                     /locus_tag="ACHLAG_001821"
     CDS             25573..26433
                     /gene="yjiK"
                     /locus_tag="ACHLAG_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFP0655066.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            26501..27679
                     /gene="yjiJ"
                     /locus_tag="ACHLAG_001822"
     CDS             26501..27679
                     /gene="yjiJ"
                     /locus_tag="ACHLAG_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0655067.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPLLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(27692..28246)
                     /gene="kptA"
                     /locus_tag="ACHLAG_001823"
     CDS             complement(27692..28246)
                     /gene="kptA"
                     /locus_tag="ACHLAG_001823"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFP0655068.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            28496..29179
                     /gene="yjiH"
                     /locus_tag="ACHLAG_001824"
     CDS             28496..29179
                     /gene="yjiH"
                     /locus_tag="ACHLAG_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFP0655069.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            29176..29637
                     /gene="yjiG"
                     /locus_tag="ACHLAG_001825"
     CDS             29176..29637
                     /gene="yjiG"
                     /locus_tag="ACHLAG_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFP0655070.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            29650..30822
                     /gene="iadA"
                     /locus_tag="ACHLAG_001826"
     CDS             29650..30822
                     /gene="iadA"
                     /locus_tag="ACHLAG_001826"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP0655071.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVNGTFETA"
     gene            30887..31798
                     /gene="hypT"
                     /locus_tag="ACHLAG_001827"
                     /gene_synonym="qseD"
     CDS             30887..31798
                     /gene="hypT"
                     /locus_tag="ACHLAG_001827"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFP0655072.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(31791..32183)
                     /gene="iraD"
                     /locus_tag="ACHLAG_001828"
     CDS             complement(31791..32183)
                     /gene="iraD"
                     /locus_tag="ACHLAG_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFP0655073.1"
                     /translation="MMRQSLQAVLPEISGNKTSSLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYSHTLAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(32180..32263)
                     /gene="idlP"
                     /locus_tag="ACHLAG_001829"
     CDS             complement(32180..32263)
                     /gene="idlP"
                     /locus_tag="ACHLAG_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFP0655074.1"
                     /translation="MENEHQYSGARCSGQTAYVAKRQECAK"
BASE COUNT         7419 a         8552 c         8508 g         7905 t
ORIGIN      
        1 cgcaatcggg aataaaagta atcattaaca tattaatgat taaaatgatc gaatccgagg
       61 agtggtaatg aaaggcacta tcttcgccgt agcgttgaac catcgcagcc aacttgatgc
      121 atggcaggaa gcgttccagc aatcccccta caaagccccg cctaaaactg cggtctggtt
      181 tattaaaccg cgcaatacgg tgattggttg cggtgaaccg attccctttc cacagggtga
      241 aaaggtactg agcggtgcga ctgttgcgct gattgtggga aaaacggcga cgaaaatacg
      301 tgaagaagat gcagcagagt acatcgccgg atatgcgctg gctaacgatg tcagcctgcc
      361 ggaagagagc ttttaccgcc cggcaatcaa agcaaaatgt cgtgatggat tctgccccat
      421 tggcgaaacc gtggctctca gcaatgtcga taatctgacc atctataccg agatcaacgg
      481 gcgtcctgcc gatcactgga ataccgccga tttacaacgt aacgccgcgc agttgctgag
      541 cgccctgagc gaatttgcca cactgaatcc aggcgatgcc attctgctcg gcacgccaca
      601 ggcgcgcgtg gaaatacagc caggcgatcg cgttcgtgtt ctcgcagaag gtttcccgcc
      661 gctggaaaat ccggtagtgg acgaacgtga agtgaccacg cgcaagagct tcccaacgca
      721 gccacacccg cacggcacgc tgtttgccct cggcctgaac tacgccgacc acgccagcga
      781 actggaattt aagccaccgg aagaaccgct ggtgttcctg aaagcgccaa ataccctcac
      841 tggcgataac cagacctccg tgcgtccaaa caatattgaa tacatgcact atgaagcgga
      901 gctggtggta gttattggca aacaggcgcg taacgtcagc gaagccgatg ccatggatta
      961 tgtcgcgggc tacaccgtgt gtaacgacta cgccattcgc gactatctgg aaaactacta
     1021 ccgccctaac ctgcgggtca aaagccgcga cggactgacg ccgatgcttt caaccatcgt
     1081 gccgaaagag gcgatcccgg acccgcataa tctgaccctt cgcaccttcg tcaacggcga
     1141 gttacgccag caaggcacca ccgccgatct gatcttcagc gtgcccttcc tgatcgccta
     1201 cttaagcgaa tttatgaccc tgaatccggg cgacatgatc gccaccggca caccaaaagg
     1261 cttatctgac gtggtgcctg gcgatgaagt ggtggtggaa gtagaaggcg tgggccgcct
     1321 ggtgaaccga attgtgagtg aggaaacagc gaaatgaaaa aagtaaatca ttggatcaac
     1381 ggtaaaaatg ttgcaggtaa cgactacttc cagactacca atccggcaac gggtgaagtg
     1441 ctggcggatg tggcctccgg cggtgaagcg gagatcaatc aggcggtagc ggcagcgaaa
     1501 gaggcgttcc cgaaatgggc caatctgccg atgaaagagc gcgcgcgcct gatgcgccgt
     1561 ctgggcgatc tgatcgacca gaacgtgccg gagatcgccg cgatggaaac cgcggacacg
     1621 ggcctgccga tccatcagac caaaaatgtg ttgatcccac gcgcttctca caactttgaa
     1681 tttttcgcgg aagtctgcca gcagatgaac ggcaagactt atccggtcga cgacaagatg
     1741 ctcaactaca cgctggtgca gccggtaggc gtttgtgcac tggtgtcacc gtggaacgtg
     1801 ccgtttatga ccgccacctg gaaggtcgcg ccgtgtctgg cgctgggcaa taccgcggta
     1861 ctgaaaatgt cggaactctc cccgctgacc gctgaccgcc tgggtgagct ggcgctggaa
     1921 gccggtattc cggcaggcgt gctgaacgtg gtacagggct acggcgcaac cgcaggggat
     1981 gcgctggttc gtcatcatga cgtacgtgcc gtgtcgttca ccggcggtac ggccaccggg
     2041 cgcaacatca tgaaaaacgc cgggctgaaa aaatactcca tggaactggg cggtaaatcg
     2101 ccggtgctga tttttgaaga tgccgatatt gaacgcgcgc tggacgccgc cctgttcacc
     2161 atcttctcga tcaacggcga acgctgcacc gccggttcgc gcatctttat tcagcaaagc
     2221 atctacccgg aattcgttaa acgctttgcc gaacgcgcca accgtctgcg cgtgggcgat
     2281 ccgaacgatc cgaataccca ggttggcgcg cttattagcc agcagcactg ggaaaaagtc
     2341 tccggctata tccgtctcgg cattgaagaa ggcgcaaccc tgctggcggg cggcccggat
     2401 aaaccgtccg acctgcctgc gcacctgaaa ggcggcaact tcctgcgccc aaccgtgctg
     2461 gcagacgttg ataaccgtat gcgagtcgcc caggaagaga ttttcgggcc ggtcgcctgc
     2521 ctgctgccgt ttaaagacga agccgaaggc ttacgtctgg caaacgacgt ggagtacggc
     2581 ctcgcgtcgt acatctggac acaggatgtc agcaaagtgt tacgcctggc gcgtggcatt
     2641 gaagctggca tggtgttcgt caacacccag aacgtgcgtg acctgcgcca gccatttggc
     2701 ggcgtaaaag cctccggcac cgggcgtgaa ggcggtgagt acagcttcga agtgttcgcg
     2761 gaaatgaaga acgtctgcat ttccatgggc gaccatccaa ttccgaaatg gggagtctga
     2821 tatgggtaag ttagcgttag ccgccaagat cacgcacgta ccgtcgatgt atctctctga
     2881 actgccaggg aaaaaccacg gttgccgcca gggcgcgatc gacgggcata aagagatcag
     2941 caagcgttgc cgggaaatgg gcgtcgatac cattatcgtt ttcgataccc actggctggt
     3001 caacagtgct tatcacatca actgtgcaga ccattttgaa ggcgtctaca ccagcaacga
     3061 gctgccgcat tttatccgcg atatgaccta caactacgag ggcaacccgg agttggggca
     3121 gcttattgcc gatgaagcct taaagctcgg cgtgcgggca aaagcgcaca acattcccag
     3181 cctgaaactg gaatacggca cgctggtgcc aatgcgctac atgaatgaag acaagcactt
     3241 caaagtggtt tccatttcag ctttctgcac tgttcacgat tttgccgaca gccgcaagct
     3301 gggcgaggcg attctgaaag cgatcgaaca gtacgacggc accgtggcgg tccttgccag
     3361 cggttcgtta tcgcaccgct ttattgacga tcagcgagcg gaagaaggga tgaacagcta
     3421 cacccgcgag ttcgaccgcc agatggacga gcgtgtggtg aagctgtggc gcgaaggcca
     3481 gttcaaagag ttctgcaata tgctgccgga gtacgccgac tactgctacg gcgaaggcaa
     3541 tatgcacgac acggtgatgc tgctggggat gctcggctgg gataaatacg acggcaaggt
     3601 ggagtttatc accgagctat tcccaagctc tggcaccggt caggttaacg ctgttttccc
     3661 gcttcccgcg taaggacgtt ttatgccgca ctttatcgtt gaatgcagtg acaacatccg
     3721 cgaagaagcc gacctgccgg ggttgttcgc caaagtgaat ccgacgctgg cagccacggg
     3781 tatttttccg ctggcgggta ttcgcagccg cgtgcattgg gtcgatacct ggcagatggc
     3841 cgacgggcag cgtgattatg ccttcgtgca tatgacgttg aaaatcggcg caggtcgcag
     3901 cctggaaagc cgtcagcagg cgggtgaaat gctgtttgaa ctgattaaaa cgcacttcgc
     3961 cgccctgatg gagagccgcc tgctggcgtt gtcgtttgag attgaagagc tgcatccgac
     4021 gctgaatttt aaacaaaaca acgtgcacgc attgtttaag tagcgcgcag attgcccggt
     4081 ggcgctgcgc ttaccgggcc tacaaaaccc caaaccgtac accgtaggcc ggataaggcg
     4141 cagccgcatc cggcaatgcc acaggatatc gctatgttcg ataaacatac ccacaccctg
     4201 atcgcccagc gtctggatca ggcagaaaaa cagcgcgaac agatccgcgc gatctcgctg
     4261 gattacccgg agatcaccat cgaagacgct tacgcggtgc agcgtgaatg ggttcgcctg
     4321 aaaatagccg aaggtcgcac gctgaaaggc cacaaaatcg gcctgacctc gaaagcgatg
     4381 caggccagct cacaaatcag cgagccggat tacggtgccc tgctggatga catgttcttc
     4441 cacgatggca gcgatatccc gaccgatcgc tttatcgtgc cgcgcattga agtggagctg
     4501 gctttcgtgc tggcaaaacc gctgcgtggg ccaaactgca cgctgttcga cgtctacaac
     4561 gccacggact acgtgatccc ggcgctggag ctgatcgacg ctcgctgcca caacatcgat
     4621 ccggaaaccc agcgcccgcg caaagtgttc gacaccattt ctgataacgc cgccaacgcc
     4681 ggggtgatcc tcggtggtcg tcccattaag cccgatgagt tggatctgcg ttggatctcc
     4741 gccctgatgt atcgcaatgg cgtgattgaa gaaaccggcg tcgccgctgg tgtgctaaat
     4801 catccggcaa acggcgtggc ctggctggcg aacaaactcg caccgtatga cgtacaactg
     4861 gaagccgggc aaatcattct cggcggttcg ttcacccgcc cggttccggc gcgtaagggc
     4921 gacaccttcc acgtcgatta cggcaacatg ggctccatta gctgccgctt tgtttaagga
     4981 gagaacgatg gaaaacagtt ttaaagcggc gctgaaagca ggccgcccgc agatcggatt
     5041 atggctgggg ctgagtagca gctacagcgc agagttactg gccggagcag gattcgactg
     5101 gttgttgatc gacggtgagc acgcgccgaa taacgtgcaa accgtgctca cccagctaca
     5161 ggcgattgcg ccctacccca gccagccggt agtacgtccg tcgtggaacg atccggtgca
     5221 aatcaaacaa ctgctggacg tcggcacaca aaccttgctg gtgccgatgg tacaaaacgc
     5281 tgacgaagcc cgtgaagcgg tacgcgccac ccgttatccc cccgccggta ttcgcggtgt
     5341 gggcagtgcg ctggcccgcg cctcgcgctg gaatcgtatt cctgattacc tgcaaaaagc
     5401 caacgatcaa atgtgcgtgc tggtgcagat cgaaacgcgt gaggcaatga agaacttacc
     5461 gcagattctg gacgtggaag gcgtcgacgg cgtgtttatc ggcccggcgg atctgagcgc
     5521 cgatatgggt tatgccggta atccgcagca cccggaagta caggccgcca ttgagcaggc
     5581 gatcgtgcag atccgtgaat cgggcaaagc gccggggatc ctgatcgcca atgagcaact
     5641 ggcaaaacgc tatctggaac tgggcgcgct gtttgtcgcc gtcggcgttg acaccaccct
     5701 gctcgcccgc gccgctgaag cgctggcagc acggtttggt gcgcaggcta ccgccgtgaa
     5761 gcccggcgtg tattaatgcc ggatgcgctg cgcttatccg gcctacactc gcaccaaatc
     5821 gtaggccgga taaggcgtta cgccgcatcc agcaaaaacc ttgtcgtacc ctacaaaaat
     5881 cccattagag gaagaaaaat gagcgacacc tcacctgcca taccggagag tatcgatccg
     5941 gcgaatcagc ataaagcgct gactgccgga caacaggcgg ttattaagaa gctatttcgc
     6001 cgcctgatcg tctttctgtt cgtgctgttt atcttctcgt tccttgatcg catcaacatc
     6061 ggctttgccg gactcacgat gggacgcgac ctcggtctga gcgccaccat gtttggcctc
     6121 gctaccaccc tgttctacgc cgcttatgtc atcttcggca ttcccagcaa cattatgctg
     6181 agtattgtcg gtgcgcggcg ctggatcgcc accatcatgg tgctttgggg catcgcctct
     6241 actgccacca tgtttgccac tggccccacc agcttgtacg tactgcgtat actggttggc
     6301 attaccgaag ccggctttct gcctggcatt ctgctgtatt taaccttctg gtttccagcc
     6361 tatttccgcg cccgtgccaa cgccttgttt atggttgcaa tgccggtaac gacagcgttg
     6421 ggatcgctcg tttccggcta cattttgtcg ctggatggcg taatggcatt aaaaggctgg
     6481 cagtggctgt ttttgctgga aggcttcccg tcggtattac tcggcgtcat ggtgtggttc
     6541 tggcttgatg actcaccgga caaagctaag tggctgacga aagaagacaa aaaatgcctg
     6601 caagagatga tggataacga tcgtctgacg ctggttcagc cagagggagc catcagccac
     6661 cacgccatgc aacaacgcag catgtggcgg gagatcttca ctccggtggt gatgatgtat
     6721 accctggcgt atttctgcct gaccaacaca cttagtgcga tcagcatctg gacaccgcag
     6781 atcctgcaaa gctttaatca gggcagcagt aatatcacca tcggcctgct ggccgccgta
     6841 ccgcagattt gtaccattct cgggatgatc tactggagcc gtcactcaga tcgccgccag
     6901 gaacgaaggc atcacaccgc ccttccttat ttgttcgctg ccgcgggttg gttactggct
     6961 tcggcaactg atcacaacat gatccagatg ctggggatca ttatggcttc gaccggatca
     7021 ttcagcgcaa tggcgatttt ctggacaaca ccggatcagt ccatcagcct gcgggcacga
     7081 gcgatcggta ttgcggtgat caacgccact ggcaacattg gttcagcgtt aagtccgttt
     7141 atgatcggct ggttgaaaga tctgaccggc agctttaaca gtggattgtg gtttgttgcc
     7201 acgctgctgg tgattggtgc ggggattatc tgggcgattc caatgcagtc ctcccgtccg
     7261 cgagcgaccc cgtaaggaac gacgatgtgt gaccgtcaga ttgccaatat tgatatcagc
     7321 aaagagtacg atgaaagcct gggcacggac gatgtgcatt atcagtcgtt cgcccgcatg
     7381 gcggcatttt ttggccgcca tatgctgcca catcgccacg aacagtactt tcagatgcat
     7441 ttcctcaata gcggacagat tgagctacag cttgacgatc atcgctactc ggtggaagcg
     7501 cccctgtttg tcctgacgcc accgtcagta cctcatgcgt ttattacgga gtctgacgcc
     7561 gacggtcatg tgttgacggt acgggaagat ctgatctggc ccctgctgga agttctttat
     7621 ccgggcactc gggaaacctt cggcctgccg gggatttgcc tgtcactggc agataaaccc
     7681 gacgaactgg cggcgctgga acactattgg caactgatag agcgggaatc ggtggaacaa
     7741 ctgcctggac gggaacacac cctgacgtta ctggcacagg cagtgttcac cctactgctg
     7801 cgtaacgcaa aactcgacga ccatgccgcc agcgggatgc gcggagaatt aaaactgttc
     7861 cagcgtttta atatgctgat tgaaagccat tttcatcagc actggacagt accggattac
     7921 gctaacgaac tgcatatcac cgaatcacga ctcacggaca tctgccgtcg ctttgccaac
     7981 cgtccgccaa aacggttgat tttcgacagg cagctgcgag aagccaagcg gctgctgctg
     8041 ttttctgata acgccgtgaa caatattgcc tggcaactcg gttttaagga tccggcttat
     8101 tttgcgcgct tttttaatcg cttagtcggt tgctcgccca gtgcttatcg cgccaaaaaa
     8161 gtacctgtga cgtgaaaatc ccttagcctt aacgggaaac caggcaccac ctgctattcc
     8221 cctttctccg cctggagcaa ggggatttag taacgtcaaa aatcgctaaa agcgacctcg
     8281 atcacaaaac cgtacccgac ctgaaaagta ccagccattg tcccaaaagt ctcttcccgt
     8341 aatcgcccct gcaagcttca attgaataac aaaaacaaca cacaaaatta acaattgtat
     8401 tccgattaat actgtagagg tcgacatgaa accagaagat ttccgcgcca gtacccaacg
     8461 tccgttcacc ggggaagagt atctgaaaag cctgcaggat ggtcgcgaga tctatatcta
     8521 tggcgagcga gtgaaagacg tcactactca tccggcattt cgtaatgcgg cagcgtctgt
     8581 tgcccaactg tacgacgcgc tgcacaaacc ggagatgcag gactctctgt gctggaacac
     8641 cgacaccggc agcggcggct atacccataa attcttccgc gtggcgaaaa gtgccgacga
     8701 cctgcgccag caacgcgacg ccatcgctga gtggtcacgc ctgagctatg gctggatggg
     8761 ccgtacccca gactacaaag ctgctttcgg ttgcgcactg ggcgcgaatc cgggctttta
     8821 cggtcagttc gagcagaacg cccgtaactg gtacacccgt attcaggaaa ctggcctcta
     8881 ctttaaccac gcgattgtta acccaccgat cgatcgtcat ttgccgaccg ataaagtaaa
     8941 agacgtttac atcaagctgg aaaaagagac tgacgccggg attatcgtca gcggtgcgaa
     9001 agtggttgcc accaactcgg cgctgactca ctacaacatg attggcttcg gctcggcaca
     9061 agtgatgggc gaaaacccgg acttcgcact gatgttcgtt gcgccaatgg atgccgatgg
     9121 cgtgaaatta atctcccgcg cctcttatga gatggtcgcg ggtgctaccg gctcgccata
     9181 cgactacccg ctctccagcc gcttcgatga gaacgatgcg attctggtga tggataacgt
     9241 gctgatccca tgggaaaacg tactgatcta ccgcgatttc gatcgctgcc gtcgctggac
     9301 aatggaaggc ggttttgccc gtatgtatcc gctgcaagcc tgtgtgcgcc tggcagtgaa
     9361 attagacttc attacagcac tgctgaaaaa atctctcgaa tgtaccggca ccctggagtt
     9421 ccgtggtgta caggccgatc tcggtgaagt ggtggcgtgg cgcaacacct tctgggcatt
     9481 gagtgactcg atgtgttctg aagcgacgcc gtgggtcaac ggggcttatt taccggatca
     9541 tgccgcactg caaacctatc gcgtactggc accaatggcc tacgcgaaga tcaaaaacat
     9601 tatcgaacgc aacgttacca gtggcctgat ctatctccct tccagtgccc gtgacctgaa
     9661 caatccgcag atcgaccagt atctggcgaa gtatgtgcgc ggttcgaatg gtatggatca
     9721 cgtccagcgc atcaagatcc tcaaactgat gtgggacgcc attggcagcg agtttggtgg
     9781 tcgccacgaa ctgtatgaaa tcaactactc cggtagccag gatgagattc gcctacagtg
     9841 tctgcgccag gcacaaagct ccggcagtat ggacaagatg atggcgatgg ttgatcgctg
     9901 cctgtcggaa tacgaccaga acggctggac tgtgccgcac ttgcacaaca acgacgatat
     9961 caacatgctg gataagctgc tgaaataacg cagcaggagg ttaagatgca attagatgaa
    10021 cagcgcctgc gctttcgtga cgcgatggcc agcctgtcgg cagcggtaaa tattatcacc
    10081 accgagggcg acgccggaca atgcgggatt acggcaacgg ccgtctgctc ggtcacggat
    10141 acaccaccat cgctgatggt gtgcattaac gccaacagtg cgatgaaccc ggtttttcag
    10201 ggcaacggta agttgtgcgt caacgtcctc aaccatgagc aggaactgat ggcacgccac
    10261 ttcgcgggca tgacaggcat ggcgatggaa gagcgtttta gcctctcatg ctggcaaaaa
    10321 ggtccgctgg cgcagccggt gctgaaaggt tcgctggcca gtcttgaagg tgagatccgc
    10381 gatgtgcagg caattggcac acatctggtg tatctggtgg agattaaaaa catcatcctc
    10441 agtgcagaag gtcacggact tatctacttt aaacgccgtt tccatccggt gatgctggaa
    10501 atggaagctg cgatttaagc tccagcccct tcacgcattc gtcgaagggg catttttatt
    10561 gtgatctcaa tcacatatcc acccgatgaa taattcttgc ccaaccgctc accgccattc
    10621 ctgaccgctt acgtccctaa aataaccact cagttattta ccttacttta cgcgcgcgta
    10681 actctggcaa catcactgca ggatagcggt caatttacct cctcaaacgc aacgcaaacc
    10741 tagaacggct tcggcctatg attaacaaat tactctatga attttggttt gcaggaaggc
    10801 agcaagtgag tgaatccccg ggagcttaca acagtaagtg acaggggtga acgaacgcaa
    10861 ctgccgcacc tgtaagctaa aagacgacga gtaaaatgcc aggttttact atggatacga
    10921 aaaaactatt caagcacata ccctgggtga ttctcggaat catcggtgca ttctgcctcg
    10981 cggtagttgc attacgtcgg ggggagcacg tcagcgccct gtggatcgtg gtcgcctctg
    11041 tgtcggtgta tctggtggcg tatcgctact acagtctgta catcgcccag aaggtgatga
    11101 aactcgaccc cacgcgcgcg acgcctgcgg ttattaacaa cgacggtctg aactacgttc
    11161 cgaccaaccg ttacgtgttg tttggtcacc acttcgccgc tatcgccggt gctggtccgc
    11221 tggtcggtcc ggttctcgcc gcacagatgg gctacctgcc tggcacgctg tggctgctgg
    11281 cgggggtagt actggccggt gcggttcagg actttatggt gctgtttatc tcctctcgcc
    11341 gtaacggcgc atctctcggt gagatgataa aagaagagat ggggccagtt ccggggacta
    11401 tcgcgctgtt tggctgtttc ttaatcatga tcatcatcct cgccgttctg gcgctgattg
    11461 tggttaaagc cttggccgaa agtccgtggg gtgtcttcac cgtttgctca accgtaccga
    11521 ttgcgctgtt tatgggtatc tacatgcgct ttatccgtcc ggggcgtgtg ggtgaagtct
    11581 ctgtcattgg tatcgtgctg ctggttgcct ctatctactt cggtggcgtg attgctcacg
    11641 atccgtactg gggtccggca ctgaccttta aagacaccac cattaccttc gcgctgattg
    11701 gctatgcgtt tgtttccgca ctgctgccgg tgtggctgat cctcgcaccg cgcgactatc
    11761 tggcaacctt cctgaaaatc ggcgttatcg tcggcctggc gctgggtatc gtggtgctga
    11821 acccggaact gaaaatgcct gcgatgaccc agtacattga cggtactggc ccgctgtgga
    11881 aaggcgctct gttcccgttc ctgttcatca ccatcgcctg tggtgcggta tctggcttcc
    11941 acgcgctgat ctcttccggt acgacgccaa aactgctggc taacgaaacc gacgcgcgtt
    12001 tcattggcta cggcgcaatg ctgatggagt ccttcgtggc gattatggcg ctggttgcag
    12061 cgtccatcat cgaaccgggt ctttacttcg cgatgaatac cccgcctgct ggccttggca
    12121 tcaccatgcc taacctgcat gaaatgggtg gcgagaacgc gccgatcatc atggcgcagc
    12181 tgaaagacgt taccgcacac gcggcagcga ccgtcagctc ctggggcttc gtgatttcgc
    12241 cagagcagat cctgcaaacc gcgaaagaca ttggtgagcc ttctgtcctg aaccgtgcag
    12301 gtggtgcgcc aacgctggcg gtaggtatcg ctcacgtgtt ccacaaagtg ctgccgatgg
    12361 ctgacatggg cttctggtat cacttcggta ttctgttcga agccctgttc atcctgaccg
    12421 cgctggatgc gggtactcgt tctggccgct ttatgctgca agacctgctg ggtaacttca
    12481 tcccgttcct gaaaaaaacc gactctctgg ttgccggtat catcggtact gcgggctgtg
    12541 tgggtctgtg gggctacctg ctgtatcagg gcgtggtcga tccgctgggc ggcgttaaga
    12601 gcctgtggcc gctgttcggt atttccaacc agatgctggc agccgtagcg ctggtactgg
    12661 gcaccgttgt gctgattaag atgaagcgca cccaatacat ctgggtaact gttgttccgg
    12721 ctgtatggct gcttatctgc accacctggg cgctgggtct gaaactgttc agcaccaacc
    12781 cgcagatgga aggcttcttc tacatggcaa gccagtacaa agagaagatt gctaacggta
    12841 ctgacctgac ggcgcagcag attgccaaca tgaaccacat cgttgtgaac aactacacca
    12901 acgcaggcct gagtattctg ttcctgattg tggtgtacag catcatcttc tacggtttca
    12961 aaacctggct gaatgtgcgt aacagcgaca aacgtactga caaagaaaca ccgtacgttc
    13021 caatcccgga aggcggcgtg aagatctctt cgcaccacta accgtattta gccccgcttc
    13081 ggcggggctt tgttcttatc agagtgaact atgtttggta acttaggaca ggcaaaaaaa
    13141 tatctcggcc aggcggcgaa gatgttgatt ggcattccag actacgacaa ctacgtcgag
    13201 catatgaaga ccaaccatcc cgacaagccg tacatgagct atgaagaatt cttccgcgaa
    13261 cgccagaacg cacgctacgg cggcgatggt aaaggcggta tgcgctgttg ttaatggaga
    13321 gagcatgaac ccgattgcag ttaccctact caccggtttt ttaggcgcgg gcaaaaccac
    13381 cctgctgcgc catattctta atgaacaaca cggctacaag attgccgtga ttgaaaacga
    13441 attcggcgaa gtctccgttg atgatcaatt gattggtgat cgcgcaacgc agatcaaaac
    13501 gctgaccaac ggctgcatct gctgttcgcg ctccaacgag ctggaggacg cgctactcga
    13561 cctgctggat aatctcgaca agggcaatat tcagttcgat cgtctggtca ttgaatgcac
    13621 cggcatggcc gatcctggcc cgattattca aacctttttc tcccatgaga ttttatgcca
    13681 gcgctacctg ctggacggcg tgattgcgct ggtggatgcg gtacatgccg atgagcagat
    13741 gaaccagttc accatcgccc agtcccaggt gggctacgcc gaccgtattc tgctgactaa
    13801 aaccgacgtc gcaggcgaag cagaaaaact gcgtgaacgc ctggcgcgca tcaacgcccg
    13861 tgcaccggtc tacaccgtca cccacggcga catcgacctg ggcctgctgt tcaacaccaa
    13921 cggttttatg ctggaagaaa acgtcgtcag caccaaaccg cgtttccact ttatcgcgga
    13981 taaacaaaac gatatttcgt cgattgtggt ggaactggat tacccggtag atatcagcga
    14041 agtttcccgg gtgatggaaa acctgctgct ggaatcggcg gataaactgc tgcgttacaa
    14101 agggatgctg tggattgacg gcgaacctaa ccgcctgctg ttccagggcg tccagcgcct
    14161 ctacagcgcc gactgggaca ggccatgggg cgatgaaaca ccgcatagca cgatggtgtt
    14221 tatcgggatt cagttgccgg aggatgagat tcgggcggcg tttgcgggac tcaaaaagta
    14281 accagccctc caacatcaca atacctgaga ctttatcacc ctcatattcc tccagaatat
    14341 gagggctttc gggcttaatt atctctgaac gtgcaacact tcctgcgact ccttatgcgc
    14401 ctccacatcc tgcttcgtta accagagcga cttacggcca aaattttcgt acattttcag
    14461 ctgatcttca tagtgcttat caactgttcc atcgggagca ataaacccac tctgaccggg
    14521 tgcgaccaca tcccaggcaa gcacaggacg atcgcttgtc gttggtgaga aaacaatcat
    14581 atcgttttct gttccacggt tttgatactc cgcctgatga cgcgtttctt ccgctgcggc
    14641 ctgcggtaca ccaaagaaat tatttgcccg gaacgttaag gccattgcag gtgttttcca
    14701 gttactcaca ttattgccat agcgtttgga aagagtctcc caggtatctt ccagcgcagc
    14761 caacacaacc tcctgctgtg gtttcccagc aaacagatca accgcctgtg ggattggtga
    14821 tttgtctccc tgcaccgcct catacaaaat ttttgctcca acacttatat tcagcgaacc
    14881 agttgggccg tcctgggttg tttcgtagcc actggcgctg taccacttat caaatggcat
    14941 aggtacggca gccactacgg tacgcttcaa catactggtc agccaaacgt tcaggatggc
    15001 agagcctggc tgctgccagg ttttaccatc atcattaagc aaattgatgc catcccaacg
    15061 tgttaatgtt tctaccaact gacgacgcgg atcgctctgt gtcaaaccag atgtcgctgc
    15121 ttgcagagta ggtaaaaaaa gcctcaggtt aagatcctga cgactggttt ggcgaataac
    15181 atcccatgcc tgatcagcag ttaagcgtgg cttttgctca agcagtcggt cgatctccgt
    15241 aacgcgatct gcaccacccc acaaaaaggc aaacagatct gaagcgggat aatctttttg
    15301 gggagaattg ttccagttag caatatatcc cgactggggg ttatacacct tagggttcat
    15361 ttcaaaaggc aatagccctt tccagtccca ttttcccgta ccaggaacgg gtaatcgcgg
    15421 atcatggcct gattgacgat ctggataagc accagtatga acataaccaa tattgccgtt
    15481 tacatcagca tagtaccagt tgatggtcag tgcttgtttc gctgcctgct gtgtccactc
    15541 ctgccaattt ttggccttca tctgatgagt ccaggccagc aaagacgcca cctctttacc
    15601 atcccatgcg cgggatttag cgtaagccgt ttgtgtcgtc tggtcagttt ggagaatgtt
    15661 gccatgcacc gtacgccaga cagtaaaggt ctctgcctga ccatttttca ccgtaatggt
    15721 ttcctcacgg cttaacattt tcacccactt accattatgc aagtagtagc ctggtttctc
    15781 tgccgacagc cgttcagcaa aaatatcgac atcatcgccg aaacctgccg ttgatcccca
    15841 ggaaatcaca ccattatgac caaaaaccag cccaggatag gcaaatggtg tattgccagt
    15901 gacatcataa ccagcaccgt gcagaccaat accataagta tacgcaggcg cataccagcc
    15961 aaactgcgga ccatttacca tgattgcttt cgcatcctgg gctttgcttt tgccgatcac
    16021 ccacatattg ctggtcgttg gataccccgc cagaccattg gcaccaccct gtgcaaattg
    16081 tgcagcaata gtttcccggt tcttccctgc tgttaacgcc agcagtgcgc catccgcccc
    16141 ttttgctggt cggtcaagca ttggtgcagg taaatcgtag cgtggcaaca gagctgctgt
    16201 ttgcgagttt tgctgattaa atttaagtgg gtagttactc tcttgtacgg caatagtggt
    16261 tggcgctgat gggtttacca gccatttcaa ctgattaaat accgccatgc cttgtgatac
    16321 accatattta tcttttaaag ccgttagcag tgccagatta tcaatttcgc tagtgctatc
    16381 agagaagcgg tttgccatgg tgcccacaaa tatcatcgcg acatcaaacg gttcccagcg
    16441 cttaggagta aagccaaatg tattaaactg ttttggtaag agcgtctctg gattggtatt
    16501 taccttatca atccaggcat tcattccatc agcgtagcct tgcagaatgg acatatcctc
    16561 tggggaaagg gcagcaattt gcgcccggat agcatccggc cagtagttac gacggatatc
    16621 tttatcaaat ttcacaaaat ctttgccaag cacttccgcg acagtccctt gagtactgcg
    16681 acgtgccatt tccatctgaa aaaggcgatc ttgtgctact acatagccat agccataaaa
    16741 taggtgccat gtatcattgg cataaatatg cggcatgccg tattcatcgc gaacaatctt
    16801 tatctcactt gacgactgct cagccagtgc aggtaagctc caataataca tcagggaagc
    16861 agtaacacag ttcacgatca tacgatttct atttttcatt gtatcctctg gcaggtgtat
    16921 aattagcgaa ttgatactag caacgaagct tcggttatct atttgcagag aattgtttca
    16981 ttatgaactg tgagcgcgat aactaatttt tgatacaaaa atttcattaa tgtgatctaa
    17041 aacgaatcgc tttattcacc gctgatgatt ttgttctatt cctttttgtg acagatcccc
    17101 cggacgcata gatgatgacc cccagagaga cagctcaggt cattatctgt atgtaaatca
    17161 gaaaaatatg ttgtaaacga cttttatggt tggattttgt agcaattttg aatgcagaca
    17221 ggcagcattt gccaacacgc cgcccatagt tcactccaca tcctccctgc gtaacccaat
    17281 atccctcaac cgttcatcac tcatctgctg taacactcgt cgggtctgca tttgccgccg
    17341 ccaacgcctc accgcatgcc agagttgcac aagtctgata aacggcgttt tggctttgtt
    17401 ttcgtgaaat tccatctgct gcctcctcat ctttcagagg cttcatcatc cgccttctgg
    17461 cagttcacaa tacagatgca aatattagtt ttatttaaca tacagacagg ttaaaacagc
    17521 atctgcatcc ggttttttag cctcatctgt accggttttt ctttctgtat gggccaaata
    17581 aggaatacag catgacgcgt tatcaacatc tggcgactct acttgccgag cggattgagc
    17641 aagggctgta tcgtcacggg gagaaattgc cgtcggtgcg cagcttaagt caggagcacg
    17701 gcgtcagcat cagcaccgtg caacaggcgt atcagacgct ggaaacgatg aggctcatca
    17761 ctccgcagcc gcgttcgggt tattttgtcg cacaacgtaa agcccagccg cctgtaccgc
    17821 cgatgacgcg tccggtgcag cgcccggtgg aaattaccca gtgggatcag gtgctggata
    17881 tgctggaggc gcatagcgac aattccattg ttccgttaag caaaagcacg ccggatgtcg
    17941 aaacgcccag cctgaaaccg ctgtggcgtg agctaagccg ggtggtgcag cataatctgc
    18001 aaaccgttct cggttatgac ttgttagccg gtcagcgagt gttgcgggag cagattgccc
    18061 gcctgatgct cgacagcggc tcggtggtca ccgccgatga catcatcatc accagcggct
    18121 gccataactc gatgtcgctg gcgttaatgg cggtgtgtaa accgggcgat attgtcgcgg
    18181 tcgaatctcc ctgttattac ggttcgatgc agatgctgcg aggcatgggc gtgaaagtga
    18241 ttgaaatccc aaccgatcca gaaactggca tcagcgttga agcactggaa ctggcgctgg
    18301 aacagtggcc gattaaaggc atcattctgg tgccaaactg taataatccg ctgggattta
    18361 ttatgccgga cgcacgcaaa cgggccgttc tctctctcgc tcagcgtcat gatattgtga
    18421 tttttgaaga tgatgtctac ggcgaactgg caacggagta tccgcgcccg cggaccatcc
    18481 attcatggga tatcgacggg cgagtgctgt tgtgcagctc gttcagtaaa agtattgctc
    18541 caggcctgcg cgtgggttgg gtcgcaccgg ggcgttatca cgataaactg atgcatatga
    18601 aatacgccat cagcagcttt aatgtgccgt ccacgcaaat ggcggcggca acgtttgtgt
    18661 tggaaggcca ctatcatcgc catatccggc ggatgcggca gacttatcag cgcaatctgg
    18721 cgctttatac ctgctggata cgggaatatt ttccctgcga aatctgtatt acgcgcccga
    18781 aaggcggatt tttactgtgg atagaattgc ctgaacaggt cgatatggtc tgcgttgcgc
    18841 ggcagctgtg ccgcatgaat atccaggtgg cagcaggctc gattttctcg gcttccggca
    18901 aataccgtaa ttgtctacgc atcaactgcg ctttgccgct cagcgaaacc tatcgcgaag
    18961 cactaaagca aattggcgag gccgtgtatc gggcaatgga ataaattcga attagtggcg
    19021 aagcacctga tttcctctca cgaatcgcct gtaactcgtt gtatctttgc agggtcatcg
    19081 ccacgttcca gcatggctta ggcaaagtgt gcttcgttca taccggatgc ggcgtgaacg
    19141 ccttatccgg cctacaaacc gcgcaaattc aatagattgc agaggaaatg taggtctgat
    19201 aagcgtagcg catcaggcag ctttgcgttt gtcatccgtc tctgattagt attcgatcca
    19261 tggatcgcgc ttgatttcag gcaagggctg ctctaatgcc tgcatttgct ctgtcgaaag
    19321 tccggtaatc ttttgtatcc attcaggatc tgccccattc tgcaacaaca atctaaggag
    19381 gcgttgttcc ccttcttctt tccccttttc catccccaac agccatccat cattataaat
    19441 tcgctctgca atcgtcatta ttcgctccct gtgttgtggc attcgacggg taagttcgct
    19501 gataaacgcc ttaaaacgcg cttcatcgcc agtcagtaaa atgtaattta acagcgcagt
    19561 tatctggctg tcattagcac actctgtaac cagtaatact accagttggt caataagccc
    19621 catcagatcg cgctggcgaa tatgcttttg gatcaactcc aacagggcga ctctgcgatg
    19681 ctgcacaatc tcgtcgtctg gcacgacagt gacatccacc agcgggaacg ctgcgctata
    19741 aagtttccgt gcggtagtcg gggcggcaaa ttcgtccagc cagcacaggg accaggggta
    19801 aggactacgg ctaccgtgat aaaacagcat cgggataacc agcggtagcg gccggcgttt
    19861 atcatgctcg atatggcgct gcatcaccgc catggaatat cgcatcaggc gaaacgccat
    19921 atgaatatcc tcgcggctct gatgttcaat cactacgtaa atataaccat caccttcacg
    19981 ggtctttacc gaccacagaa tatcggagtg tagcgcccgc aatttttcat cgacaaagct
    20041 ggcggattcc agttttaagc tgtcgagatc gcacagttca cgtaaatctt tgggtaagtg
    20101 gatctccata aaatcccgcg cggtgtcagg gtgagtgaga aaggatttaa ataatgcgtc
    20161 atgtggcgtg ctggtcgtga agtttgtcat ggcgatccgt cacctgtaga taagatggcc
    20221 cgactttagc gactaacaaa accattctca cccggcaatt tctcgttccc cgaggtgctt
    20281 tccaggctat ttttgttcgt ctgcaaacga tacatttttc cggaaacagg ctcgcgtttt
    20341 acatttcctg tacccggcag atattcccct ttccaccgcg ctcttttgat taaccgcaaa
    20401 ttttaattac acttaaggtg tatattttct atgcaaccca tcaattaaag aggtgtaatg
    20461 tgctgatgac tatttgtaat cgttatacat tctgacctgg agtcagaagg tatttctctg
    20521 tctgtgtgtt cacaggcagt gtggttgatt acatgaaatt cagtacattt gtccttctcg
    20581 cctgcctttc gtcattaccg acggtattga atttcgtttt ccccgttggg gttctccgga
    20641 caaggagttg tttgttatgc cacgtttttt tgcccgccat gccgccacgc tgtttttccc
    20701 gatggcgttg attttgtatg actttgccgc gtacctgtcg acggatctga tccagccggg
    20761 gatcattaat gtggtccgtg attttaatgc cgatgtcagt ctggccccgg cagccgtcag
    20821 tctctatctc gcgggcggta tggcgttaca gtggctgctg gggccgcttt ccgacaggat
    20881 tggccgcagg ccggtgctga ttaccggggc gctaatattt acccttgcct gcgctgcgac
    20941 aatgttcaca acgtctatga cacagtttct tatcgcgcgt gcaattcagg gcaccagtat
    21001 ctgttttatt gccaccgttg gttatgtcac ggtgcaggag gcgttcggcc agacaaaagg
    21061 gatcaagttg atggcgatta tcacctccat cgtactgatt gcgccgatta ttggcccgct
    21121 ttccggcgca gctctgatgc actttgtgca ctggaaagtc ctttttgcca tcattgcagt
    21181 tatgggtttt atctcatttg ttggcttact gctggcgatg ccagagacgg tgaagcgcgg
    21241 cgcggttcca tttagcgcca aaagcgtctt tcgcgatttt cgtaatgtct tttgcaatcg
    21301 gctgttcctc tttggcgcag caaccatctc tttaagctat atcccaatga tgagttgggt
    21361 ggctgtctcg ccggtgatcc tgatcgacgc gggcggctta acaacttcgc agttcgcctg
    21421 gacacaggtt ccggtgttcg gcgcggtgat tgtcgcgaat gccatcgtgg cgcgtttcgt
    21481 taaagatccg accgaaccgc gctttatctg gcgtgctgta cccattcaac tggtcggcct
    21541 cgcgctgttg attatcggca atctgctgtc accgcacgtc tggctgtggt cggtgctggg
    21601 caccagtttg tatgctttcg ggattggttt gatcttcccg accttattcc gctttacgct
    21661 tttttccaat aacttaccga aagggaccgt ctccgcatcg ctgaatatgg tgatcctgat
    21721 ggtgatgtct gtctcggtcg aaatcggccg ctggctgtgg tttaacggcg gtcgcttgcc
    21781 gtttcatctg ttagccgtcg tggcgggcgt tatcgtcgtt ttcaccctgg caggattgct
    21841 caatcgcgtg cgccaacatc aggctgccga gctggcggag gagcggtgat ttttgcgcga
    21901 tcctgccgtc aggctctatt cttaacgtta tgaataaact cattgaactc agacgcgcca
    21961 aaatgttggc gctctcttta ctgcttatcg ccgctgctac ctttgtcgtt acgctgtttt
    22021 tgccgcccaa tttttgggtg agcggcgtga aggcgattgc tgaagcggcg atggtcggcg
    22081 cgctggcgga ctggtttgcg gtggtggcgc tgtttcgccg cgtgccgatt ccgatcattt
    22141 ctcgtcatac ggcgattatc ccgcgtaata aagaccggat tggcgaaaat ctcggccagt
    22201 tcgtgcagga aaaatttctc gatacccagt cgctggtggc attgattcga cgccacgaac
    22261 cggtgttgtt gattggcaac tggtttagtc agccagaaaa cgcccgccgc gttggtcagc
    22321 atctgttgca gatcatgagc ggttttcttg aactgaccga tgatgcgcgt attcagcgcc
    22381 tgcttaagcg cgcggtccat cgggcgattg ataaggtcga tctttccggc accagtgcgt
    22441 tgatgctgga gagtatgacc aaaaacgatc gtcatcaggt gctactggat acgctgatcg
    22501 cacagttgat cgcccttctc cagcgcgata aatcgcgcaa gtttattgcc cagcaaattg
    22561 ttcgctggct ggagagtgag catccactga aagccaaaat tctccccacc gaatggttgg
    22621 gcgaacatag cgcggagttg gtttctgacg cggtgaattc tttgcttgat gatatcagcc
    22681 gtgatcgtgc gcatcagatc cgtcatgcgt ttgatcgcgc cacttttgcc ctgatcgaca
    22741 agttgaaaaa cgatccggaa atggcagcgc gagccgatgc cgtaaaaagt tatctgaaag
    22801 aagatgaagc ttttaaccgc tatctcagtg aattgtgggg ggatttacgg gagtggctga
    22861 aagcggatat caacagtgaa gattctcgtg tgaaagaacg tatcgcgcgg gcgggtcaat
    22921 ggtttggcga aacgttaatt gccgatgatg ccttgcgggc gtcgttaaat ggtcacctgg
    22981 aacaagccgc gcaccgcgtc gcgcctgagt tttccgcatt cctgacgcgc cacatcagcg
    23041 atacagtaaa aagctgggat gcgcgggata tgtcgcggca aatcgagtta aatatcggca
    23101 aagatctgca gtttatccgt gtcaacggta cgctggttgg cggttgtatt gggctaattt
    23161 tatatttgtt gtcgcagctc ccggccttgt tccccctcag caatttttag aaatccatga
    23221 aaaactaagt ggctaaccgc gagagagatc aaataaacca cactaaaaga atggtaataa
    23281 tgtggcctct tttcattatc tcccgtggta cggagaagga aaatcatgtc acttatcacc
    23341 gatctacccg ccattttcga tcagttctct gaagctcgcc agaaaggctt tctcaccgtc
    23401 atggatctca aggagcgcgg cattccgctg gttggcactt actgcacctt tatgccgcaa
    23461 gagatcccga tggcagccgg tgcggttgtg gtttcgctct gttctacctc tgatgaaacc
    23521 attgaagaag cggagaaaga tctgccgcgc aacctctgcc cgctgattaa aagcagctac
    23581 ggctttggca aaaccgataa atgcccctac ttctactttt cggatctggt ggtcggtgaa
    23641 accacctgcg acggcaaaaa gaaaatgtat gaatacatgg cggagtttaa gcccgttcat
    23701 gtgatgcagt tgccgaacag cgttaaggac gatgcctcgc gtgcgttatg gaaagccgag
    23761 atgctgcgct tgcaaaaaac ggtagaagaa cgttttgggc acgagattag cgaagatgct
    23821 ctgcgcgatg ccattgcgct gaaaaaccgc gaacgtcgcg cactggctaa tttttatcat
    23881 cttgggcagt taaatcctcc ggcgcttagc ggcagcgaca ttctgaaagt ggtctacggc
    23941 gcaaccttcc ggttcgataa agaggcgttg atcaatgaac tggacgcgat gacagcccgc
    24001 gttcgtcagc agtgggaaga aggccagcgg ctggccctgc gtccacgcat tttaatcacc
    24061 ggctgcccga ttggcggcgc agcagaaaaa gtggtgcgcg cgattgaaga gaatggcggc
    24121 tgggttgtcg gttatgaaaa ctgcaccggg gcgaaagcga ccgagcaatg cgtggcagaa
    24181 acgggcgatg tctacgacgc actggcggat aaatatctgg cgattggctg ctcctgtgtt
    24241 tcgccgaacg atcagcgcct gcaaatgctc agccagatgg tggaagaata tcaggtcgat
    24301 ggcgtagttg atgtgatttt gcaggcgtgc catacctacg cggtggaatc gctggcgatt
    24361 aaacgtcatg tgcgtcagca gcacaacatt ccttatatcg ctattgaaac agactactcc
    24421 acctcagatg tcgggcagct cagtacccgt gtcgcggcct ttattgagat gctgtaagga
    24481 gtggcagtga catattcgat tggcattgat tctggttcta ccgccaccaa agggatctta
    24541 ctggcagacg gcgtgattac gcgccgtttc ctcgttccaa caccctttcg tccggcaaca
    24601 gcaattactg aagcctggga aactctgcgc gaagggttag agacaacgcc gtttctgacg
    24661 ctcaccggct atggacggca actggtggat tttgccgata aacaagtgac ggaaatctcc
    24721 tgtcatgggc tgggcgcacg gtttcttgcg ccagcaacgc gcgcggtaat cgacatcggt
    24781 ggtcaggaca gcaaagtgat tcagcttgat gatgacggta acctgtgcga tttcctgatg
    24841 aatgacaaat gcgcggcggg caccgggcgt ttcctggagg tgatctcgcg cacgcttggc
    24901 accagcgtcg agcaactcga cagcattacc gaaaatgtca cgccgcacgc catcacgagt
    24961 atgtgcacag tgtttgccga atcagaagtg atcagcctgc gctcagcggg cgtcgcgcca
    25021 gaagcgattc tcgccggagt gattaacgcg atggcgcgga ggagtgccaa tttcattgct
    25081 cgtctctcct gtgaagcgcc gattctgttt actggtggcg ttagtcattg ccagacgttt
    25141 gcccggatgc tggaatctca tctgggaatg ccggtaaata cccatcctga tgcgcaattt
    25201 gctggcgcaa ttggcgcggc ggtaattggt caacgagtga ggacacgccg atgaaagagt
    25261 ttttattttt atttcactcc acggtcggcg tcatacaaac ccgcaaggcg ttgcaggcag
    25321 cgggcatgac ttttcgcgtc agcgatattc cccgtgattt acgcggcggc tgcgggttat
    25381 gcatttggct gacctgtccc cccggcgagg aaatacaatg ggtgatccct gggcagactg
    25441 agtcaattta ttgccagcag gatggtgtct ggcgctgcat tgcacattac ggggtttctc
    25501 cgcgttaagg attcattaag gtcgctacgt atacttctcg atatcttatt gatttgcctt
    25561 aaagggatta ccgtgacaaa aagtatttcg ttaagtaaac gcatctccgt tattgtgatt
    25621 ctttttgcca ttgtggcagt gtgtactttc ttcgtgcaat cttgcgcccg taaaagcaat
    25681 catgcagcat catttcagaa ttaccacgcc accattgacg gcaaagaaat tgcaggcata
    25741 acgaataata tctcttcgct gacctggtcg gcacaaagca atactctttt tagcactatc
    25801 aacaaacctg ccgccattgt tgaaatgacc accaatggtg attttatccg cacaattcct
    25861 ctggattttg ttaaggatct cgagacgatc gaatacattg gcgataatca gtttgtcatt
    25921 agcgatgagc gcgattacgc catttatgtg atttcactga ccccaaactc ggaagtaaaa
    25981 atcctcaaaa aaataaaaat cccgctgcaa gactccccaa ccaattgcgg ttttgaaggg
    26041 ttggcttatt ctcgccagga tcacactttc tggtttttta aagagaaaaa tccgattgag
    26101 gtctacaaag ttaacgggct gttaagtagc aatgaattac acatcagcaa agacgaggca
    26161 ctacaacggc aatttacttt agatgatgtg tccggcgcag aatttaacca gcaaaaaaat
    26221 acgcttctgg tgctatctca tgaatctcgc gcactccagg aagtaaccct ggttggcgag
    26281 gttatcggtg aaatatcact aaccaaaggt agtcgaggtt tgtcgcataa catcaaacag
    26341 gccgaaggga ttgcgatgga tgcctctggt aatatttata tcgttagcga accaaatcgt
    26401 ttttatcgct ttacccctaa atcatcgcat taacaggctg gcatatttca cctttttgtt
    26461 attaacttgt taacaacaaa aatgacatac aaggttaaac atgccttcgt cctcgcatcc
    26521 cgtagaacgc ttttctttca gcaccgcgct tttcgggatg ctggttctga ccttaggtat
    26581 gggattaggt cgcttccttt atacgcctat gttgcccgtc atgatggcgg aaggggcgtt
    26641 ttcatttagc cagctctcgt ggattgccag cggcaactat gcggggtatc tggctggcag
    26701 tctgctattt tcgtttggcg catttcacca gccgtcgcgc ctgcgcccat tactgttggt
    26761 ttctgccctg gcgagcgggt tgttgatcct cgcaatggca tggttgccgc catttattct
    26821 ggtgttattg attcgcttcc tggcgggtgt cgccagcgcc ggtatgctga tttttggttc
    26881 gacgctgatt atgcagcaca cgcgccatcc ttttgtgctg gcagctttgt tttctggcgt
    26941 tggcattggc attgcactgg gcaacgaata tgttctggca ggcctgcatt ttgccttctc
    27001 ctcgcaaacg ttatggcaag gcgccggggc gctttctggc atgatgctga ttgcacttac
    27061 gcttttaatg ccctcgaaaa aacacgccat cgcaccaatg ccattggcaa aaacggagca
    27121 acagataatg agctggtggt tactggctat tctgtacggc cttgcgggat ttggttatat
    27181 catcgtcgcg acttacctgc cgctcatggc gaaagatgca ggctcaccat tgttaaccgc
    27241 ccatctctgg acgttagtcg gcttatcaat tgtgccaggc tgctttggct ggctatgggc
    27301 agcaaaacgt tggggggctt tgccttgcct gacggcgaat ttgctggtgc aggctatcag
    27361 tgtgctgctt actctcgcca gcgactctcc tctcttgctt atcattagta gtatcggttt
    27421 tggcggtacc tttatgggca cgacatctct ggtgatgact atcgcccgcc aactgagcgt
    27481 tccgggaaat ctcaatcttt taggctttgt gacgctcatt tacggcattg gacaaatcct
    27541 cggcccggcg ctgaccagta tgctcggcaa tggaacatcg gcgcttgccg gcgccactct
    27601 ctgcggcgcg gcggcgttgt ttattgctgc gcttatcagc acagtacaat tatttaagct
    27661 gcaagtggtc acttcatgag gagtgtttcc cttaccactc aagaaactca acagcaaccg
    27721 ttgatgtcag ccagaccccg ttttccgctt gccagaaagg gatgcctcgt ttcgccattt
    27781 cctgtgcttt gacggttaag ataaccggcg atccgtgtct tgctcccact ttgcgtgccg
    27841 ttgcttcatc agcagagaga tgtacgtaat gacgctcccc tgcaatcagc ccctgttttt
    27901 ttatctcgtc aaggaaacgg cttgccgtac cgtggtagag aaactgtggc ggtgtttttt
    27961 cggcaaagga aatggcgacc tgcgaagtcg aatgcccctg aaccgcgcgg atgcacctac
    28021 catcactgga ataactaaaa cgctttttat cgctggtggc aaccacagta tccagcaatg
    28081 cacgggttaa ccgcttgccc gctttttggg cacaaagtat gagcttatcg atatccgccc
    28141 atccttcacg gtcaagtacg ataccaatcg cctccggttt gtgtcggagg acaaaactta
    28201 aaaacttgct cgtatccgcc aactcttttt cgttgtactt tgccatcgcg ttttcgtccc
    28261 tggattttct gtttttattt actgatgtca ttgtcactgg gtttatgaat tttctgcaac
    28321 tcaatcctgc gcagagcctt ttctacacag cgtaattttt ccgctatgca aaaaacgcat
    28381 agcaagaaac atacttgcat tttccccata cccacatggc tgataaggtt tttcagctcc
    28441 aggaattttc ctggctcaag caacacacaa acacaaaaaa acaatgggga ttgttatgac
    28501 acaacaaggg gatgctgtag ccggggagct ggcgacagaa aaagtgggca ttaaagggta
    28561 tctggctttt tttctcacca taatcttttt ttccggagtt ttttccggga cagacagctg
    28621 gtggcgcgtt tttgattttt ccgttctgaa cggatcgttt gggcaactgc ctggtgcgaa
    28681 tggagccacc acctcatttc gcggggcggg tggcgcgggg gctaaagatg gctttctctt
    28741 tgcgctggag ctggcaccgt cagtcattct ctctctgggg attatttcaa ttaccgatgg
    28801 tcttggtgga ttacgcgccg cgcagcagtt aatgacgcct gtgctaaaac cgttgttggg
    28861 cattccaggg atctgctcgc tggcgctgat cgctaacctg caaaacactg atgccgccgc
    28921 cggtatgacc aaagagctgg cgcaggaagg ggaaattacc gaacgcgata aagtgatttt
    28981 cgccgcatat caaaccagcg gtagcgccat catcaccaac tacttctctt ccggcgtcgc
    29041 ggtgttcgcc tttctgggca cgtccgtaat cgtaccttta gccgtcatcc tggtgtttaa
    29101 atttgtcgga gccaacattt tgcgcgtctg gcttaacttc gaagaacgtc gcaacccaac
    29161 gcaaggagcg caagcatgac aactcaggtc cgcaaaaacg tcatggatat gtttatcgat
    29221 ggcgcgcgcc gtggctttac tatcgccacg accaacctgc tgccaaacgt ggtgatggcg
    29281 tttgtcatca ttcaggcgct gaaaattacc ggcctgctcg actgggtggg gcatatctgt
    29341 gaacccgtaa tggcgctgtg gggattaccc ggtgaagcgg caaccgtcct tttagccgca
    29401 ttaatgagta tgggcggcgc agtcggtgtt gccgccagtc tggcgaccgc cggcgcatta
    29461 accggtcacg atgtcaccgt tttactgccc gcgatgtatt taatgggcaa cccggtgcaa
    29521 aatgtcggtc gctgcctggg gacagccgaa gtgaatgcca aatattaccc gcatatcatt
    29581 acggtctgcg tgattaacgc attgctgtcg atctgggtta tgcagcttat tgtttaacaa
    29641 ggagttacca tgattgatta taccgcagcc ggttttaccc tgctgcaggg agcgcatttg
    29701 tatgcgccgg aagatcgggg aatttgcgat gtcctcgtcg ctaacggcaa aattatcgcc
    29761 gttgccagca atatcccttc tgacattgta ccggactgca cggttgtcga tctcagcggg
    29821 cagatcctct gtcctggttt tattgatcaa cacgtccatt tgattggtgg tggcggcgaa
    29881 gcaggtccca cgacgcgcac gccagaagtg gcgctaagtc gcctgacgga agcgggcgtt
    29941 acgtcagtgg ttggtctgct gggcaccgac tctatctctc gccacccgga atccctgctc
    30001 gccaagaccc gtgcgctcaa tgaagaaggc atcagcgcct ggatgctgac cggcgcttat
    30061 catgtccctt cccgcaccat tacaggttcc gtggaaaaag acgtggcgat tatcgatcgt
    30121 gtgattggcg tgaaatgcgc catctctgat caccgttctg ccgcaccgga cgtttatcac
    30181 ctggctaata tggcggcgga atcccgcgtt ggcggtttgc tcggcggtaa acctggcgtc
    30241 accgtgttcc acatgggcga cagcaaaaag gcgttacagc ctgtctatga cctgctggaa
    30301 aactgcgatg tgccgatcag caagttgctg ccgacccacg ttaaccgcaa cgttccgttg
    30361 tttgagcagg cgctggagtt cgcgcgcaaa ggcggcacta tcgatatcac cagcagcatt
    30421 gacgaaccgg tcgcccctgc cgaaggtatt gcccgcgccg ttcaggcggg tattccgctg
    30481 gcacgcgtca ccctcagctc cgacggcaac ggtagccagc cgttcttcga tgacgaaggg
    30541 aatttaaccc atatcggtgt tgccggtttt gaaacgttgc tggaaaccgt gcaggtgctg
    30601 gtcaaagact atgatttcag tatcagcgat gccctgcgcc cgctcaccag tagcgtagcc
    30661 ggtttcctta acctgaccgg gaaaggcgaa attctgccag gcaatgatgc tgacttgctg
    30721 gtcatgacgc cagaactgcg cattgagcag gtatacgctc gcggcaaact gatggtcaaa
    30781 gacggcaaag cctgcgtgaa tggaacgttt gaaacggctt aaacggttgt cagccgcccg
    30841 aatggacgta gaatgcccgc aagggcggct gacagagtaa aacgtaatgg atgactgtgg
    30901 tgcgattttg cataatattg aaaccaaatg gctttatgat tttctgaccc tggaaaaatg
    30961 ccgcaatttt tcccaggcgg cagtcagtcg caacgtctcg caaccggcat tcagccgccg
    31021 catccgtgcg ctggaacagg cgattggtgt tgaattgttt aaccgccagg taacgccgct
    31081 gcaactctca gaacaaggga aaatcttcca ttcgcagatc cgccatctgt tgcaacagtt
    31141 agagagcaat ctggcagagc tgcgtggcgg cagcgattac gcgcaacgaa aaatcaagat
    31201 agccgctgca cactctcttt ccctcgggct gttaccgtcc attatcagcc agatgccgcc
    31261 gctctttacc tgggcaattg aagctattga tgtcgatgaa gcggtcgata aactgcgtga
    31321 agggcaaagt gactgtattt tttcctttca cgacgaagat ttgctggaag cgccgtttga
    31381 ccacattcgc ttatttgaat ctcaattgtt ccctgtctgc gccagtgacg aacacggaga
    31441 agcacttttt aacctcgcgc agccacactt tccgttactg aattacagcc gcaactccta
    31501 catggggcga ttgattaatc gcaccctgac gcgccacagt gagttaagtt tcagcacctt
    31561 ttttgtctct tcgatgagcg agcttttaaa gcaggttgcc ctcgacggct gtgggattgc
    31621 ctggctgccg gagtacgcca tccaacaaga aattcgcagc gggaagctcg ttgtactgaa
    31681 tcgggacgaa ctggtgatcc cgattcaggc ctatgcatac cgaatgaata cccgcatgaa
    31741 tccagttgcc gaacgcttct ggcgtgaact gcgcgagctg gagattgtgc ttagctgaca
    31801 ttctccagca tcgcactaca ccaaccgcca ttatcccaga ccagatcaaa cgttagcggt
    31861 tcaccgtcgt ggtacagcat tatctcaagg ctgatcgcca gcgtgtggct gtacgcttct
    31921 ttcagattga cctgcaacgc cgcaatccgt ggctcatgaa ataacagtgc agaacgcagc
    31981 tgacggcaat accagctctg gagatgtttg tcgctctggt tatgcacttg ccactcgggc
    32041 atgcctgaaa caagtaatga cggtaatgcc gaatgaggag aattaaaaag agtgaggaga
    32101 tcgctacata ctgatttacg cagcgatgac gttttattgc ctgaaatttc aggtaaaaca
    32161 gcctgaagtg attggcgcat cattttgcgc actcctgacg tttagcaaca tatgcggttt
    32221 gccctgaaca ccgggcacca ctgtattgat gctcattttc catagccatt cccttttgtt
    32281 cattacatcc atgtcagtct gagacaggcc tgaatcctgt cagcaaagta taaaacctta
    32341 cttgcctatg aatatctact gacttttcag gaaaaaagtc atga
//
LOCUS       DAZGVQ010000040        32287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000040 DAZGVQ010000000
VERSION     DAZGVQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.040"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(38..1798)
                     /gene="ycaO"
                     /locus_tag="ACHLAG_001830"
     CDS             complement(38..1798)
                     /gene="ycaO"
                     /locus_tag="ACHLAG_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFP0655075.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1928..2620
                     /locus_tag="ACHLAG_001831"
     CDS             1928..2620
                     /locus_tag="ACHLAG_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFP0655076.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2819..3907
                     /gene="serC"
                     /locus_tag="ACHLAG_001832"
     CDS             2819..3907
                     /gene="serC"
                     /locus_tag="ACHLAG_001832"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFP0655077.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIECPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            3978..5261
                     /gene="aroA"
                     /locus_tag="ACHLAG_001833"
     CDS             3978..5261
                     /gene="aroA"
                     /locus_tag="ACHLAG_001833"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP0655078.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5430..6194
                     /gene="ycaL"
                     /locus_tag="ACHLAG_001834"
     CDS             5430..6194
                     /gene="ycaL"
                     /locus_tag="ACHLAG_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFP0655079.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6367..7050
                     /gene="cmk"
                     /locus_tag="ACHLAG_001835"
     CDS             6367..7050
                     /gene="cmk"
                     /locus_tag="ACHLAG_001835"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFP0655080.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7161..8834
                     /gene="rpsA"
                     /locus_tag="ACHLAG_001836"
     CDS             7161..8834
                     /gene="rpsA"
                     /locus_tag="ACHLAG_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFP0655081.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            8994..9278
                     /gene="ihfB"
                     /locus_tag="ACHLAG_001837"
     CDS             8994..9278
                     /gene="ihfB"
                     /locus_tag="ACHLAG_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFP0655082.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9485..11748
                     /locus_tag="ACHLAG_001838"
                     /pseudo
     CDS             9485..11748
                     /locus_tag="ACHLAG_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            11785..13533
                     /gene="msbA"
                     /locus_tag="ACHLAG_001839"
     CDS             11785..13533
                     /gene="msbA"
                     /locus_tag="ACHLAG_001839"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFP0655083.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13530..14516
                     /gene="lpxK"
                     /locus_tag="ACHLAG_001840"
     CDS             13530..14516
                     /gene="lpxK"
                     /locus_tag="ACHLAG_001840"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFP0655084.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            14553..15785
                     /gene="ycaQ"
                     /locus_tag="ACHLAG_001841"
     CDS             14553..15785
                     /gene="ycaQ"
                     /locus_tag="ACHLAG_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFP0655085.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            15837..16019
                     /gene="ycaR"
                     /locus_tag="ACHLAG_001842"
     CDS             15837..16019
                     /gene="ycaR"
                     /locus_tag="ACHLAG_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFP0655086.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16016..16762
                     /gene="kdsB"
                     /locus_tag="ACHLAG_001843"
     CDS             16016..16762
                     /gene="kdsB"
                     /locus_tag="ACHLAG_001843"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFP0655087.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            16916..17809
                     /locus_tag="ACHLAG_001844"
     CDS             16916..17809
                     /locus_tag="ACHLAG_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFP0655088.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17786..18565)
                     /gene="elyC"
                     /locus_tag="ACHLAG_001845"
     CDS             complement(17786..18565)
                     /gene="elyC"
                     /locus_tag="ACHLAG_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFP0655089.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            18701..19486
                     /gene="cmoM"
                     /locus_tag="ACHLAG_001846"
     CDS             18701..19486
                     /gene="cmoM"
                     /locus_tag="ACHLAG_001846"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFP0655090.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDHVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19483..20805
                     /gene="mukF"
                     /locus_tag="ACHLAG_001847"
     CDS             19483..20805
                     /gene="mukF"
                     /locus_tag="ACHLAG_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFP0655091.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20786..21490
                     /gene="mukE"
                     /locus_tag="ACHLAG_001848"
     CDS             20786..21490
                     /gene="mukE"
                     /locus_tag="ACHLAG_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFP0655092.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21490..25950
                     /gene="mukB"
                     /locus_tag="ACHLAG_001849"
     CDS             21490..25950
                     /gene="mukB"
                     /locus_tag="ACHLAG_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFP0655093.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVSKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADCAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26211..28058
                     /gene="ldtD"
                     /locus_tag="ACHLAG_001850"
     CDS             26211..28058
                     /gene="ldtD"
                     /locus_tag="ACHLAG_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP0655094.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRLSDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28239..28787
                     /locus_tag="ACHLAG_001851"
     CDS             28239..28787
                     /locus_tag="ACHLAG_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFP0655095.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28814..29461
                     /gene="gloC"
                     /locus_tag="ACHLAG_001852"
     CDS             28814..29461
                     /gene="gloC"
                     /locus_tag="ACHLAG_001852"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFP0655096.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29683..30873)
                     /gene="aspC"
                     /locus_tag="ACHLAG_001853"
     CDS             complement(29683..30873)
                     /gene="aspC"
                     /locus_tag="ACHLAG_001853"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFP0655097.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(31058..32146)
                     /gene="ompF"
                     /locus_tag="ACHLAG_001854"
     CDS             complement(31058..32146)
                     /gene="ompF"
                     /locus_tag="ACHLAG_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFP0655098.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7842 a         7856 c         8692 g         7897 t
ORIGIN      
        1 ctaatcatta agcctacatt gcgtaggcta tttgatttta ttttgcccag aatgctgctt
       61 tggcgcgctg cagcttttcg taggccttca acaacgactg atgtgcagca aacgcgtgca
      121 gatcgctatc taccggttgc aggccgtaaa acgccgcttc gccgctcatt gccgcactgg
      181 cggcttctac ggcatctgcg ccgtacatgc gaacaaacgc attcagatat tgcagcggct
      241 ggcgatcttc ttcctgtgcc agtaataaca acgtttgcag gcagcgataa tagttggcgc
      301 gttccggact aaatactgat gagttaaact ccatcgtcca ttcggtccag accagagcct
      361 gttccagatc gccaccagcc agcgccagca tggcttttaa ttcaccgata cgcagggtgt
      421 accaaccgtt atccgacccg gtcgccagac ccaacagctc acgcacgcgg gtaaagtcat
      481 caaaaccttc ttcatccagt tgctcgatga ggttcaggta atcttctttt tcccactcgc
      541 tgcctggtag cgaaagaatc gtttcacgta aatggctgcc catactgtta ttcgcgagcc
      601 acagatcttc agccggataa atatcggaca tgccaggcac gataatgcgg caagcgtaga
      661 cacccagatg ctcgtaatcg gcaatataaa cttctttatc ttcctttttg aagatagcca
      721 tcagcgtggc gaactcttct tccgtggtgc cggagaaatt ccagtccaca aacggataat
      781 cggcatcctg cttgaacagg tcccaggaga ttaaaccgct ggaatcgata aagtgcgttt
      841 cgaggttggt atgttcagcg acttcttcat catcgaaggt tggcggagta aacacatcca
      901 aatctttcag gccacgacct tgcagcagct cggtcacggt acgttccagt gctacgccaa
      961 aatcaggatg cgcaccgaaa gaggcaaagc aggtaccgtt agcaggattg aacagtacca
     1021 cgcaaatcac cggatactgg ccgccaagcg aaccgtcata agcgaagatt gggaaaccct
     1081 ccgcttccag cgtttcgatg gcttcaacta ctgctgggta acgcgccagc acgtctgccg
     1141 ggatctctgg caggctgatg ctttcagcaa taatgcggtt tttcacgtag cgttcgaaaa
     1201 cttcagacaa tccctgaacg cgtgcttcgt tgcgggtatt acctgcggac ataccgttgg
     1261 agacgtacag gttaccaatg atattcatcg gaatataaac ggtctgattg tcggactgac
     1321 gcgtaaacgg caggccgcaa ataccacgat cttcgttacc ggattgtagg tcaatcagca
     1381 tgctgccggt cagttcattc tccggatcat aaaacgcgcg cagacggtca tcgagtagtc
     1441 cttctggcac atcgtcattt tcggtcagtg ggaaccattt ttcgttggga taatgcacga
     1501 acggaccgtt ggcgatggtt tcgcccagcc agaaatcagc aaaaaagtag ttggttgaga
     1561 gacgctcgaa atattcacca agtgcagaag ccagcgccgc ttttttggtt gcgcctttac
     1621 cgttggtaaa acacagtgcg cactctttgt cgcgaatatg tacagaccag acgttaggca
     1681 caggattcag ccaggaggcc tcttcaatct gaaagccgag gtctgaaagt ttttgctgga
     1741 agcgagcgat ggaatcttcc agagcggcat ctttgccggg gataaatgtt tgcgtcatga
     1801 aaatcacttt agtcgtacgg aaagcgcgca ataatacggg ttttatctca aaggcgctat
     1861 caccgccgcc atgccgatga actgttgact atgcttttag cggataaacc acgataagag
     1921 cataaaaatg aaggcgttcg atctccaccg tatggcattt gataaagtgc cttttgattt
     1981 ccttggcgaa gttgcactac gtagtcttta tacctttgta ctggtttttc tgttcctcaa
     2041 aatgaccgga agacgcggtg tgcgacagat gtcgctgttt gaagtgttaa tcattctcac
     2101 gctgggttca gcggcgggag atgtggcgtt ttatgatgat gtgccgatgg tgccggtact
     2161 tatcgtcttt atcactctgg cgttgttata ccgcctggta atgtggttga tggcgcacag
     2221 tgaaaaactg gaagatttac tggaaggtaa gccggttgtt attattgaag atggcgagct
     2281 ggcctggtcg aaactcaata actccaacat gacggaattt gagttcttta tggagctacg
     2341 attgcgtggc gtggagcagc tggggcaggt acgtctggcg attctcgaaa ccaacgggca
     2401 aatcagtgtc tatttctttg aagatgacaa ggtgaaaccg ggtttactta ttttacccag
     2461 tgattgtacg cagcgttaca aagtggtgcc ggagtcggcg gactatgcct gtattcgttg
     2521 tagtgaaatc attcatatga aagcggggga aaaacaatta tgtccgcgct gtgcaaatcc
     2581 agaatggacg aaggcaagtc gggcaaaacg ggtgacctga cagtaaaaac atcggctttt
     2641 tgctaataat ccgagagatt cttttgtgtg atgcaagcca catttttgcc ctcaacggtt
     2701 ttactcattg cgatgtgtgt cactgaatga taaaaccgat agccacagga ataatgtatt
     2761 acctgtggtc gcaatcgatt gaccgcgggt taatagcaac gcaacgtggt gaggggaaat
     2821 ggctcaaatc ttcaatttta gttctggtcc ggcaatgcta ccggcagagg tgcttaaaca
     2881 ggctcaacag gaactgcgcg actggaacgg tcttggtacg tcggtgatgg aagtgagtca
     2941 ccgtggcaaa gagttcattc aggttgcaga ggaagccgag aaggattttc gcgatcttct
     3001 taatgttccc tccaactaca aggtattatt ctgccatggc ggtggtcgcg gtcagtttgc
     3061 tgcggtaccg ctgaatattc tcggtgataa aaccaccgca gattatgttg atgccggtta
     3121 ctgggcggca agtgccatta aagaagcgaa aaaatactgc acgcctaatg tctttgacgc
     3181 caaagtgact gttgatggtc tgcgcgcggt taagccaatg cgtgaatggc aactctctga
     3241 taatgctgct tatatgcatt actgcccgaa tgaaaccatc gacggtatcg ccatcgacga
     3301 aacgccagac ttcggcaaag atgtggtggt cgccgccgac ttctcttcaa ccattctttc
     3361 ccgtccgatt gacgtcagcc gttatggcgt gatttacgct ggcgcgcaga aaaatatcgg
     3421 cccggctggc ctgacaatcg tcatcgttcg tgaagatttg ctgggcaaag cgaatatcga
     3481 gtgtccgtcg attctggatt attccatcct caacgataac ggctccatgt ttaacacgcc
     3541 gccgacattt gcctggtatc tatctggtct ggtctttaaa tggctgaaag cgaacggcgg
     3601 tgtagctgaa atggataaaa tcaatcagca aaaagcagaa ctgctatatg gggtgattga
     3661 taacagcgat ttctaccgca atgacgtggc gaaagctaac cgttcgcgga tgaacgtgcc
     3721 gttccagttg gcggacagtg cgcttgacaa attgttcctt gaagagtctt ttgctgctgg
     3781 ccttcatgca ctgaaaggtc accgtgtggt cggcggaatg cgcgcttcta tttataacgc
     3841 catgccgctg gaaggcgtta aagcgctgac agacttcatg gttgagttcg aacgccgtca
     3901 cggttaatgc cgaaattttg cttaatcccc acagccagcc tgtggggttt ttatttctgt
     3961 tgtagagagt tgagttcatg gaatccctga cgttacaacc catcgctcgt gtcgatggca
     4021 ctattaatct gcccggttcc aagagcgttt ctaaccgcgc tttattgctg gcggcattag
     4081 cacacggcaa aacagtatta accaatctgc tggatagcga tgacgtgcgc catatgctga
     4141 atgcattaac agcgttaggg gtaagctata cgctttcagc cgatcgtacg cgttgcgaaa
     4201 ttatcggtaa cggcggtcca ttacacgcag aaggtgccct ggagttattc ctcggtaacg
     4261 ccggaacggc aatgcgtccg ctggcggcag ctctttgtct gggtagcaat gatattgtgc
     4321 tgaccggtga gccgcgtatg aaagaacgcc cgattggtca tctggtggat gcgctgcgcc
     4381 tgggcggggc gaagatcact tacctggaac aagaaaatta tccgccgttg cgtttacagg
     4441 gcggctttac tggcggcaac gttgacgttg atggctccgt ttccagccaa ttcctcaccg
     4501 cactgttaat gactgcgcct cttgcgccgg aagatacggt gattcgtatt aaaggcgatc
     4561 tggtttctaa accttatatc gacatcacac tcaatctgat gaagacgttt ggtgttgaaa
     4621 ttgaaaatca gcactatcaa caatttgtcg taaaaggcgg gcagtcttat cagtctccgg
     4681 gtacttattt ggtcgaaggc gatgcatctt cggcttctta ctttctggca gcagcagcaa
     4741 tcaaaggcgg cactgtaaaa gtgaccggta ttggacgtaa cagtatgcag ggtgatattc
     4801 gctttgctga tgtgctggaa aaaatgggcg cgaccatttg ctggggcgat gattatattt
     4861 cctgcacgcg tggtgaactg aacgctattg atatggatat gaaccatatt cccgatgcgg
     4921 cgatgaccat tgccacggcg gcgttatttg caaaaggcac caccacgctg cgcaatatct
     4981 ataactggcg tgttaaagaa accgatcgcc tgtttgcgat ggcaacagaa ctgcgtaaag
     5041 tcggtgcgga agtagaagag gggcacgatt acattcgtat cactccaccg gaaaaactga
     5101 actttgccga gatcgcgaca tacaatgatc accggatggc gatgtgtttc tcgctggtgg
     5161 cgttgtcaga tacaccagtg acgattcttg atcccaaatg tacggccaaa acatttccgg
     5221 attatttcga gcagttggcg cggattagcc aggcagcctg aatgaacaac gggcaataaa
     5281 tagccaaatc tttctttatc aaaacgtcgg cacattgtcg gcgttttttt tcggaccttg
     5341 tgagtcattt tgattaatgg tagcgtcgct tgtcaatgta agttgttgat acataatatt
     5401 tatatatgat taatcaacgg atgattcaca tgaagaatac taaattactg ctggcgattg
     5461 cgacctctgc agcattactg acagggtgtc aaaataccca cggtattgat accaatatgg
     5521 ctatcagctc cggtttaaat gcctataaag cagcaacatt aagcgatgcc gatgcaaaag
     5581 cgattgccaa tcagggctgt gccgaaatgg acagcggcaa tcaagtcgca agtaaatcca
     5641 gcaagtacgg taaacgtctg gcaaaaatcg ccaaagcatt gggtaacaat attaatggca
     5701 cgccggtcaa ctataaggtt tatatgacca gcgacgtcaa cgcatgggcg atggcgaacg
     5761 gctgtgtccg tgtctacagt ggcctgatgg acatgatgaa tgacaacgaa attgaaggtg
     5821 ttctgggcca tgaactgggc cacgtcgcgt tgggtcactc gctggctgaa atgaaagctt
     5881 cttatgcgat cgttgccgca cgcgatgcca tttcagctac cagcggagtg gcttcccagc
     5941 tttcccgctc acaattgggt gatatcgcag aaggcgctat taatgctaaa tactcccgtg
     6001 ataaagagtc cgaagcagat gatttctcct ttgatctgtt gaagaaacgt ggcatcagca
     6061 cccaggggct ggttggcagc tttgaaaaac tggctagtct ggatggcggt cgcacccagt
     6121 ccatgtttga ctctcaccca ccatcaacag agcgtgcgca acacatccgt gatcgtatcg
     6181 cctctggtaa gtaaatcatt gtcatctttc gggctggtct tctgccagcc cgctataatt
     6241 gcgcaataaa tccccatctg aatacagaca aaactggttt ttgcacacaa cgttaacgat
     6301 ttgtggcgtc ggcgcgtata atgcgcgcgg ttatgttaac ggtacgcctg ttttaaggag
     6361 ataaagatga cggcaattgc cccggttatt accattgatg gcccaagcgg tgcagggaaa
     6421 ggcaccttgt gtaaggctat ggcggaagca ttgcaatggc atctgctgga ttcgggtgca
     6481 atttatcgcg tactggcatt ggcggcatta catcaccatg ttgatgttgc gtcagaagat
     6541 gcgctggtac cgctggcatc ccatctggat gtacgttttg tgtcaaccaa tggcaatctg
     6601 gaagtgatcc tcgaagggga agatgtcagc ggcgaaattc gtactcagga agtggcgaat
     6661 gcagcttcac aagtcgcggc attcccacgc gttcgtgaag cattattgcg tcgccaacgc
     6721 gcgtttcgcg aattaccagg cctgattgcc gatggccgcg acatgggaac ggtggtattc
     6781 cctgatgcgc cagtgaaaat tttccttgac gcctcctcgg aagaacgtgc gcatcgccgc
     6841 atgctacagt tgcaggagaa gggctttagt gttaactttg agcgcctttt ggccgagatc
     6901 aaagaacgcg acgaccgcga tcgtaaccga gcggtagcgc cactggttcc ggcagccgat
     6961 gctttagtgt tggattccac caccttaagc attgagcaag tgattgaaaa agcgctacaa
     7021 tacgcgcgcc agaaattggc tctcgcataa gcgaccgaat ttgcagtacc cccgttgcaa
     7081 tggaatgaca gcgggtatgt taaacaaccc catccggcat ggagccaggt ggacgttaaa
     7141 tataaacctg aagattaaac atgactgaat cttttgctca actctttgaa gagtccttaa
     7201 aagaaatcga aacccgcccg ggttctatcg ttcgtggcgt tgttgttgct atcgacaaag
     7261 acgtagtact ggttgacgct ggtctgaaat ctgagtccgc catcccggct gagcagttca
     7321 aaaacgccca gggcgagctg gaaatccagg taggtgacga agttgacgtt gctctggacg
     7381 cagtagaaga cggcttcggt gaaactctgc tgtcccgtga gaaagctaaa cgtcacgaag
     7441 cctggatcac gctggaaaaa gcttacgaag atgctgaaac tgttaccggt gttatcaacg
     7501 gcaaagttaa gggcggcttc actgttgagc tgaacggtat tcgtgcgttc ctgccaggtt
     7561 ctctggtaga cgttcgtccg gtgcgtgaca ctctgcacct ggaaggcaaa gagcttgaat
     7621 ttaaagtaat caagctggat cagaagcgca acaacgttgt tgtttctcgt cgtgccgtta
     7681 tcgaatccga aaacagcgca gagcgcgatc agctgctgga aaacctgcag gaaggcatgg
     7741 aagttaaagg tatcgttaag aacctcactg actacggtgc attcgttgat ctgggcggcg
     7801 ttgacggcct gctgcacatc actgacatgg cctggaaacg cgttaagcat ccgagcgaaa
     7861 tcgtcaacgt gggcgacgaa atcactgtta aagtgctgaa gttcgaccgc gaacgtaccc
     7921 gtgtatccct gggcctgaaa cagttgggcg aagatccgtg ggtagctatc gctaaacgtt
     7981 atccggaagg taccaaactg actggtcgcg tgaccaactt gaccgactac ggctgcttcg
     8041 ttgaaatcga agaaggcgtt gaaggcctgg tacacgtttc cgaaatggat tggaccaaca
     8101 aaaacatcca cccgtccaaa gttgttaacg ttggcgatgt agtggaagtt atggttctgg
     8161 atatcgacga agaacgtcgt cgtatctccc tgggtctgaa acagtgcaaa gctaacccgt
     8221 ggcagcagtt cgcggaaacc cacaacaagg gcgaccgtgt tgaaggtaaa atcaagtcta
     8281 tcactgactt cggtatcttc atcggcctgg acggcggcat cgacggcctg gttcacctgt
     8341 ctgacatctc ctggaacgtt gcaggcgaag aagcagttcg tgaatacaaa aaaggcgacg
     8401 aaatcgctgc agttgttctg caggttgacg cagaacgtga acgtatctcc ctgggcgtta
     8461 aacagctcgc agaagatccg ttcaacaact gggttgctct gaacaagaaa ggcgctatcg
     8521 taaccggtaa agtaactgca gttgacgcta aaggcgcaac cgtagaactg gctgacggcg
     8581 ttgaaggtta cctgcgtgct tctgaagcat cccgtgaccg cgttgaagac gctaccctgg
     8641 ttctgagcgt tggcgacgaa gttgaagcta aattcaccgg cgttgatcgt aaaaaccgcg
     8701 caatcagcct gtctgttcgt gcgaaagacg aagctgacga gaaagatgca atcgcaactg
     8761 ttaacaaaca ggaagatgca aacttctcca acaacgcaat ggctgaagct ttcaaagcag
     8821 ctaaaggcga gtaattctct gactcttcgg gatttttatt ccgaagtttg ttgagtttac
     8881 ttgacagatt gcaggtttcg tcctgtaatc aagcactaag ggcggctacg gccgccctta
     8941 atcaatgcag caacagcagc cgcttaattt gcctttaagg aaccggagga atcatgacca
     9001 agtcagaatt gatagaaaga cttgccaccc agcaatcgca cattcccgcc aagacggttg
     9061 aagatgcagt aaaagagatg ctggagcata tggcctcgac tcttgcgcag ggcgagcgta
     9121 ttgaaatccg cggtttcggc agtttctctt tgcactaccg cgcaccacgt accggacgta
     9181 atccgaagac tggcgataaa gtagaactgg aaggaaaata cgttcctcac tttaaacctg
     9241 gtaaagaact gcgcgatcgc gccaatattt acggttaagt ttttactcaa acttgaacgc
     9301 gagaaaagca cctgtcgggt gcttttttca tttctctaat caggaactgg aagctgcctc
     9361 gcagagtttt gagcagtttt caccctttcg ttaaattctt ctgaatgtgc ctcggggaac
     9421 gcaaaattcc caccagacaa ccgctcaaca aagttgcaca ctttccataa acagggaggg
     9481 gtgcatgaaa ataacgacag tcggcgtatg cataatttgc ggaatttttc cgttgctgat
     9541 tttgccccaa ttgcctggga cattaaccct tgcgtttctg actctcttcg cctgcgtact
     9601 ggctttatcc ctgttaaaac cgtccgttat atcgcgctga cgttgctgtt tttcgtttgg
     9661 ggcatattat cagcaaagca aattttgtgg gcaggagaaa ccttaactgg cgcgacgcag
     9721 gatgcaattg ttgagatcac tgcaactgac ggcatgacca ctcattacgg tcaaattact
     9781 catctacaag gtcgacgtat attccctgcg ccaggccttg tgctgtatgg cgaatatctt
     9841 ccgcaagcgg tttgtgctgg acaacaatgg tcaatgaaac tcaaagttcg tgcggttcat
     9901 ggccaactta atgatggcgg ctttgatagc cagcgttatg ccattgccca gcatcaaccg
     9961 ctcaccggcc gctttctgca ggcaagtgtt attgaaccga attgtagcct gcgtgcacag
    10021 tatctggcgt cactacaaac aacgctgcaa ccctatccgt ggaatgcggt tattcttggt
    10081 ttaggtatgg gggaacggtt atccgtcccc aaagaaatca aaaatatcat gcgcgatact
    10141 ggaacggcgc atttaatggc gatatcggga ttgcacatcg cttttgcggc gttgctggct
    10201 gccggactca ttcgcagtgg acaaattttt ctgcctgggc gctggatcca ctggcaaata
    10261 ccattaattg gtgggatctg ctgtgctgct ttttatgcct ggttgacggg aatgcaacct
    10321 cctgcattgc gtaccgtggt ggcgcttgct acgtggggaa tgcttaagtt aagtgggcga
    10381 cagtggagtg gctgggatgt atggatatgt tgtctggcgg caattttgct gatggatcct
    10441 gttgccattc tctcgcaaag tttatggctc tctgccgctg cggtcgcggc actgattttt
    10501 tggtatcagt ggtttccctg tcctgagtgg caactgccgc cggtattgcg tgcagttgtt
    10561 tccctcatcc atctgcaact gggtatcaca cttctgctta tgcccgtgca aatcgtcata
    10621 tttcatggca ttagtctgac ctcgtttatt gcaaatctat tagcaattcc cttggtgaca
    10681 tttatcacgg ttccgttgat cctcgccgcg atggttgtgc atttaagcgg gccgttaatc
    10741 ctggagcaag ggttatggtt tcttgccgac cggtctttgg ctttactttt ctgggggtta
    10801 aagagtttgc cagaagggtg gatcaacatt gctgaacgtt ggcaatggct atcattttcc
    10861 ccatggttct tactggtggt atggcgatta aacgcctggc gaacgttgcc agcaatgtgt
    10921 gtggctgtag gcttgctgat gtgctggccg ctatggcaaa aacctcgacc tgacgagtgg
    10981 caggtgtaca tgcttgatgt cgggcaaggg ctggcaatgg tgatagccag aaacggcaaa
    11041 gcgattctct atgacacagg actggcctgg cccgaagggg atagtgggca acaactgatt
    11101 atcccctggc tccactggca taatcttgaa ccggaaggcg ttattctgag tcatgaacat
    11161 ctggatcacc ggggagggct ggactcaata ttgcacacat ggccgatctt atggatcaga
    11221 agtccgttag actgggaaca tcatcagccc tgtgtgcgtg gcgaagcgta gcaatggcaa
    11281 ggattgcgtt tcagcgtgca ctggccttta caagctagca acgataaagg aaataaccat
    11341 tcctgtgtgg ttaaggttga tgacgggacg aatagcattc ttctaaccgg tgatattgaa
    11401 gtccccgctg aacaaaagat gctaagccgt tactggcagc aagtgcagac aacattgctt
    11461 caggtacctc accatggcag taatacctca tcatcgttgc cattaattca gcgagtgaat
    11521 gaaaaagtgg cactcgcatc ggcatcgcgc tataacgcat ggcgattgcc ctctaataaa
    11581 gttaagcatc gctatcaaca gcaaggatat caatggcttg atactccaca tcagggtcaa
    11641 gtgacggtca atttttcagc gcaaggctgg cggattagca gcctcagaga gcaaatttta
    11701 cctcgttggt atcatcagtg gtttggcgtg ccagtggata acgggtagaa tatgcggcta
    11761 tttcaacaaa tgctggtttt ttgaatgcat aacgacaaag atctctctac gtggcagacg
    11821 ttccgccgac tgtggccaac cattgcgcct tttaaagcgg gtctgatcgt ggcgggcgta
    11881 gcgttaatcc tcaacgcagc cagcgatacc ttcatgttat cgctccttaa gccacttctt
    11941 gatgatggct ttggtaaaac agatcgctcc gtgctggtgt ggatgccgct ggtggtgatc
    12001 gggctgatga ttttacgtgg tatcaccagc tatgtctcca gctactgtat ctcctgggta
    12061 tcaggaaagg tggtaatgac catgcgtcgc cgcctgtttg gtcacatgat gggaatgccg
    12121 gtttcatttt ttgacaaaca gtcaacgggg acgctgttgt cacgtatcac ctacgattcc
    12181 gaacaggttg cttcttcttc ttccggcgca ctgataacgg ttgtgcgtga aggtgcgtca
    12241 atcatcggcc tgttcatcat gatgttctat tacagttggc aactgtcgat cattttgatt
    12301 gtgctggcac cgattgtttc gattgcgatt cgcgtagtat cgaagcgttt tcgcaacatc
    12361 agtaaaaaca tgcagaacac catggggcag gtgaccacca gcgccgaaca aatgctgaag
    12421 ggccacaaag aagtattgat tttcggtggt caggaagtgg aaacgaaacg ctttgataaa
    12481 gtcagcaacc gaatgcgtct tcaggggatg aaaatggttt cagcctcttc catctctgat
    12541 ccgatcattc agctgatcgc ctctttggcg ctggcgtttg ttctgtatgc ggcgagcttc
    12601 ccaagtgtca tggatagcct gactgccggt acgattaccg ttgttttctc ttcaatgatt
    12661 gcactgatgc gtccgctgaa atcactgacc aacgttaacg cccagttcca gcgcggtatg
    12721 gcggcttgtc agacgctgtt taccattctg gacagtgagc aggagaaaga cgaaggtaag
    12781 cgcgtgatcg agcgtgcgac tggcgacgtg gaattccgca atgtcacctt tacttatccg
    12841 ggacgtgacg tacctgcatt gcgtaacatc aacctgaaaa ttccggcagg gaagacggtt
    12901 gctctggttg gacgctctgg ttcaggtaaa tcaaccatcg ccagcctgat cactcgtttt
    12961 tacgatattg atgaaggcga aatcctgatg gatggtcacg atctgcgcga gtataccctg
    13021 gcgtcgttac gtaaccaggt tgctctggtg tcgcagaatg tccatctgtt taacgatacg
    13081 gttgctaaca acattgctta cgcacggact gaacagtaca gccgtgagca aattgaagaa
    13141 gcggcgcgta tggcctacgc catggacttc atcaataaga tggataacgg tctcgataca
    13201 gtgattggtg aaaacggcgt gctgctctct ggcggtcagc gtcagcgtat tgctatcgct
    13261 cgagccttgc tgcgtgatag cccgattctg attctggatg aagctacctc ggctctggat
    13321 accgaatccg aacgtgcgat tcaggcggca ctggatgagt tgcagaaaaa ccgtacctct
    13381 ctggtgattg cccaccgctt gtctaccatt gaaaaggcag acgaaatcgt ggtcgtcgag
    13441 gatggtgtca ttgtggaacg cggtacgcat aacgatttgc ttgagcaccg cggcgtttac
    13501 gcgcaacttc acaaaatgca gtttggccaa tgatcgaaaa aatctggtct ggtgaatccc
    13561 ctttgtggcg gctattgctg ccactctcct ggttgtatgg cctggtaagt ggcgcgatcc
    13621 gtctttgcta taaactaaaa ctgaagcgcg cctggcgtgc ccccgtaccg gttgtcgtgg
    13681 ttggtaatct caccgcaggc ggcaacggaa aaaccccggt cgttgtctgg ctggtggaac
    13741 agttgcaaca gcgcggtatt cgcgtggggg tcgtatcgcg gggatatggt ggtaaggctg
    13801 aatcttatcc gctgttattg tcggcagata ccacaacagc acaggcgggt gatgaacctg
    13861 tgttgattta tcaacgcact gatgcgcctg ttgcggtttc tcccgttcgt tctgatgcgg
    13921 taaaagccat tctggcgcaa caccctgatg tgcagatcat cgtaaccgac gacggtttac
    13981 agcattaccg tctggcgcgt gatgtggaaa ttgtcgttat tgatggtgtg cgtcgctttg
    14041 gcaatggctg gtggttgccg gcggggccaa tgcgtgagcg agcggggcgc ttaaagtcgg
    14101 ttgatgcggt aatcgtcaac ggcggtgtcc ctcgcagcgg tgaaatcccc atgcatctgc
    14161 tgccgggtca ggcggtgaat ttacgtaccg gtacgcgttg tgacgttgct cagcttgaac
    14221 atgtggtggc gatggcaggg attgggcatc cgccgcgctt ttttgccacg ctgaagatgt
    14281 gcggcgtaca accggaaaaa tgtgtaccgc tggccgatca tcagtctttg aaccatgcgg
    14341 atgtcagcgc gttggtaagc accgggcaaa cgctggtaat gactgaaaaa gatgcggtga
    14401 aatgccgggc ctttgcagaa gaaaattggt ggtatttgcc tgtagacgca cagctttcag
    14461 gtgatgaacc agcgaaactg cttacgcaac taacctcgct ggcttctggc aactagttac
    14521 gccgcggcag cgttcgattg atggagtcat gaatgtcgct gccgcacctc tcccttgctg
    14581 atgcgcgtaa tcttcacctt gccgcacaag gcctgttaaa caaaccccgc cgtcaagcgt
    14641 cgttggagga tattccggca acgatctccc gcatgtcctt gctgcaaatc gataccatca
    14701 atattgttgc ccgtagtcca tatctggtgc ttttcagtcg tctgggaaat tatcctgccc
    14761 agtggctgga tgagtctctg gcgcgtggcg aattaatgga atactgggcg catgaagcct
    14821 gctttatgcc gcgtagcgac tttcgtctta ttcgccaccg catgctggca cctgaaaaaa
    14881 tgggctggaa atacaaagac gcctggatgc aggaacatga ggcggaaatt gcacagttaa
    14941 ttcagcatat tcatgataag gggccggtac gttcagccga ttttgagcat cctcgtaaag
    15001 gtgcaagcgg ctggtgggaa tggaagccgc ataaacggca tctggaaggt ttatttactg
    15061 ccggaaaggt gatggtgatt gaacggcgca acttccagcg cgtttatgat ttaacccacc
    15121 gtgtcatgcc tgactgggat gatgagcgcg atctcgtttc gcaaacagaa gcagaaatca
    15181 tcatgctgga taacagtgcg cgtagcctgg gaatattccg cgaacagtgg ctggcagatt
    15241 actatcggct gaaacgtccg gcactggcgg cgtggcgcga agcgagggct gaacagcagc
    15301 aaatcattgc tgtgcatgtt gaaaaattgg gcaatctttg gctgcatgct gatttgctgc
    15361 cgctactcga gcgtgcgctg gccggaaagc tcactgcaac gcacagcgcg gtactttcgc
    15421 cttttgatcc tgttgtctgg gatcgcaaac gcgcagagca gctttttgat tttagctacc
    15481 ggctggagtg ctatacccca gcgccgaaac gccagtatgg ctattttgtt ctgccattat
    15541 tacatcgtgg gcaattagtt gggcgaatgg atgccaaaat gcatcgccag acaggcatcc
    15601 ttgaagttat ctctctgtgg ttacaggaag gcattaaacc aacgacaatg ctgcaaaaag
    15661 ggttacgtca ggcgattact gatttcgcga gctggcagca ggcaacgcgg gtgacattag
    15721 gacgctgccc gcaaggtctc tttactgatt gccgcactgg ctgggaaata gaccccgtcg
    15781 cataaatgat tatgataaga tttatgcatt cataacccga tccatctgga ggaactatgg
    15841 atcatcgttt gcttgaaatc attgcctgcc cggtttgcaa cggaaaactt tggtataacc
    15901 aggaaaaaca agagcttatt tgcaaactgg ataacctggc tttcccgctc cgcgatggca
    15961 ttccagtgtt gctggaaacg gaagcccgcg tgctgactgc tgatgagagt aaatcatgag
    16021 ttttgtggtc attattcccg cgcgctacgc gtccacgcgt ctgcccggta aaccattggt
    16081 tgatattaac ggcaaaccca tgattgttca tgttcttgaa cgcgcgcgtg aatcaggtgc
    16141 cgagcgcatc atcgtggcaa ccgatcatga ggatgttgcc cgcgccgttg aagccgctgg
    16201 cggtgaagta tgtatgacgc gcgccgatca tcagtcagga acagaacgtc tggcggaagt
    16261 tgtcgaaaaa tgcgcattca gcgacgacac ggtgatcgtt aatgtgcagg gtgatgaacc
    16321 gatgatccct gcgacaatca ttcgtcaggt tgctgataac ctcgctcagc gtcaggtggg
    16381 tatggcgact ctggcggtgc caatccacaa tgcggaagaa gcgtttaacc cgaatgcggt
    16441 gaaagtggtt ctcgacgctg aagggtatgc actgtacttc tcacgcgcca ccattccttg
    16501 ggatcgtgat cgttttgcag aaggccttga aaccgttggc gataacttcc tgcgtcatct
    16561 tggtatttat ggctaccgtg caggctttat ccgtcgttac gtcaactggc aggcaagtcc
    16621 gttagaacac atcgaaatgt tagagcagct tcgtgttctg tggtacggcg aaaaaatcca
    16681 tgttgctgtt gctcaggaag ttcctggcac aggtgtggat acccctgaag atcttgagcg
    16741 cgttcgcgct gaaatgcgct aatttcactt cacgacactt cagccaattt tgggaggagt
    16801 gtcgtaccgt tacgattttc ctcaattttt cttttcaaca attgatctca ttcaggtgac
    16861 atcttttata ttggcgctca ttatgaaagc agtagctttt atgagggtaa tctgaatgga
    16921 acagctgcgt gccgaattaa gccatttact gggcgaaaaa ctcagtcgta ttgagtgcgt
    16981 caatgaaaaa gcggatacgg cgttgtgggc tttgtatgac agccagggaa acccaatgcc
    17041 gttaatggca agaagcttta gtacgcccgg aaaagcccga caactggcat ggaaaaccac
    17101 catgctggca agaagtggga ctgtccgtat gccgactatt tatggtgtga tgacgcatga
    17161 agaacacccc ggccccgatg tcctgctact ggagcggatg cgtggtgttt cggtggaggc
    17221 accagcccga acaccagaac gctgggaaca actcaaagac caaatcgttg aagccttact
    17281 ggcctggcac cgtcaggaca gtcgcggttg cgtcggcgcg gtcgacaata ctcaggaaaa
    17341 tttctggccc tcatggtacc ggcaacatgt tgaagtgcta tggaccacgc tcaatcagtt
    17401 caataacacc ggtctgacga tgcaggataa gcggatcctg tttcgcactc gcgaatgtct
    17461 cccggcatta tttgaaggct ttaacgacaa ttgtgtgttg attcacggta acttctgttt
    17521 acgcagcatg ctgaaagatt cgcgcagcga tcagttactg gcgatggtcg ggccgggact
    17581 aatgctttgg gcaccgcgag aatacgaact gttccgacta atggataatt ctctggcgga
    17641 agatttgctc tggagttacc tgcaacgcgc gccagtggcg gagtcgttca tctggcggcg
    17701 ttggttgtat gtgttatggg atgaagttgc gcaactggtt aataccggac gatttagtcg
    17761 gcgcaacttc gatctggcat caaaatcact cttgccgtgg ctcgcctgac ggacctttca
    17821 gccactgcca gatgcggcca agcgtttcat agccaacacg atcgctatgc atcagccaga
    17881 ccggagaagg gattgcccgt tcccacggat taagcggcga gtcgatggcc aactgattcg
    17941 ccggggctgg tagcggattt aacccttcct gctgaaaaaa gatcatcgcc cgcggcagat
    18001 gtgatgcgga ggtcaccagc aggaaagggg cgtcaccaat cgcctgtttc actgccgcag
    18061 cttcttcttc ggtatctttt ggcaaatcca gggtgataat ttgctcgcgc ggcacgccca
    18121 gcgattgcgc aactctggca cctacttccg ctgtactcac cgtattggtt tttgctacgc
    18181 ctcccgtgaa gatcagtttt gatcccggat tttcgcgcca taagcgaata ccttcattca
    18241 ggcgtggcag actgttattg attaaattag agctcggtgc ccactgcggg ttccaggtat
    18301 aaccacctcc gagcaccacg atatagtcca ctttttgcga attattccat gtcggatagg
    18361 tgctttcgat gggacgcagc aggcggtcgg ctaccggttg caaactcaat aacaagagtg
    18421 ccagccaccc aatgctaata aaaatcttcc cggttttttg aaaacggcta aaccacagca
    18481 gcgccaggcc agcgccaata attaacaaca tcagcggaag tggcagcaac atattgccaa
    18541 tcactttttt cagtgtaaaa agcatccttt ctggttcctt ttttaaccat atagcaaggg
    18601 atcgccagcg atattacacc agacgggttc attctcgccg tggctgtgac aaaatagcgt
    18661 ctttatcaga aaacgtctta gccaaccggt ggagaaagca atgcaggatc gcaattttga
    18721 tgatattgcg gaaaagtttt cccgtaacat ttacggcacc accaaagggc agcttcgaca
    18781 ggctattctg tggcaggatc tcgatcacgt gctggcggaa atgggcccgc aaaaactgcg
    18841 tgtgctggat gctggcggtg gagaagggca gaccgcaatc aaaatggccg agcgtgggca
    18901 tcaggtcatt ttatgcgatc tttctgcgca gatgatcgac cgcgcaaaac aggcggcaga
    18961 agcaaaaggt gtgagcgaca acatgcaatt tatacattgc gccgctcagg atgttgcttc
    19021 gcatttggaa acgcccgttg atctgatatt gttccatgcg gtgctcgagt gggtggccga
    19081 tccccgcagt gtattgcaga ccctgtggtc agtgttgcgt ccaggcggcg tgttgtcgtt
    19141 aatgttctac aatgcgcatg gtttgttgat gcataacatg gtcgccggga attttgatta
    19201 cgtgcaggcg ggaatgccga aaaagaaaaa acggacgctt tcgccagatt atccacgcga
    19261 cccggcgcag gtttatctgt ggctggaaga agctggttgg caaattatgg gtaagacagg
    19321 cgttcgcgtg tttcatgatt atctgcgcga gaagcaccag cagcgcgact gctatgaagc
    19381 attacttgaa ttagaaacgc gttattgccg tcaggaaccg tatattaccc tggggcgtta
    19441 tattcatgtc accgcgcgca aaccgcagag caaggataaa gtatgagtga attttcccag
    19501 acagtccccg aactggttgc ctgggccaga aaaaatgact tctccatctc gctgccggta
    19561 gaccgactct cttttctgct ggcggttgcc acgctgaacg gcgagcgtct ggatggtgag
    19621 atgagtgaag gcgagctggt ggatgcattc cgccatgtga gtgatgcgtt tgagcaaacc
    19681 agcgaaacca tcggcgtgcg cgccaataac gcgatcaacg acatggtgcg tcaacgtctg
    19741 ctgaaccgct ttaccagcga gcaggcggaa gggaacgcaa tttaccgtct gacgccgctc
    19801 ggcatcggca ttactgacta ctacatccgt cagcgcgagt tttctacgct gcgtctttct
    19861 atgcagttgt cgattgtggc gggtgagctc aaacgcgcag cagatgccgc cgaagagggc
    19921 ggtgatgaat ttcactggca ccgtaatgtc tatgcgccac tgaaatattc ggtagcagaa
    19981 attttcgaca gtatcgacct gacgcaacgt ctgatggacg aacagcagca gcaggtgaag
    20041 gacgatatcg cccagttgct gaacaaagac tggcgggcgg cgatttccag ctgtgaattg
    20101 ttgctttcgg aaacttccgg aacgctgcgt gaattgcagg atacgctgga agcggcaggc
    20161 gacaaattgc aggctaatct gttgcgcatt caggatgcga cgatgaccca tgacgatctg
    20221 catttcgtcg atcgtctggt gttcgatctg cagagcaaac tcgatcgtat tatcagttgg
    20281 ggccagcaat ccatcgactt gtggattggc tacgaccgcc acgtacacaa atttattcgt
    20341 accgcgatcg atatggataa aaaccgcgtc tttgctcagc ggttacgtca gtcggtacaa
    20401 acctattttg atgagccgtg ggcgctaact tatgccaatg ccgatcgtct gctggatatg
    20461 cgtgacgaag agatggcact gcgcgatgaa gaagtgactg gggaacttcc tgaggatctg
    20521 gaatacgaag agtttaacga gatccgcgaa cagctggcgg cgatcatcga agaacaactt
    20581 gccgtgtaca aaaccagaca agtgccgctg gatcttggtc tggtggtacg cgaatatctg
    20641 tcacagtatc cgcgtgcacg tcactttgac gttgcgcgca ttgttattga tcaggcggta
    20701 cgtcttggcg tagcgcaagc agatttcacc ggactgccag cgaaatggca gccgattaat
    20761 gattacggag ccaaggtaca ggcgcatgtc atcgacaaat attgaacaag tgatgccggt
    20821 taagctggcg caggcgctgg cgaatccgtt atttccggcg ctggacagcg ccttacgttc
    20881 aggccgccat attggcctcg acgaactgga taatcatgca ttcctgatgg attttcagga
    20941 atatctggaa gagttttacg cgcgttataa cgttgagctt attcgcgcac cagaagggtt
    21001 cttctattta cgcccacgtt ccaccacgct gatccctcgt tccgtcttgt cggaactgga
    21061 tatgatggtc gggaaaatcc tctgttatct ctatctcagc ccggaacggc tggcgaatga
    21121 ggggattttc acccagcagg aactgtacga cgaactgctc accctggccg atgaagcaaa
    21181 actgctgaaa ctggtgaaca accgttcaac cggttcagac gttgaccgtc agaagttgca
    21241 ggagaaagta cgttcttcgc tcaaccgtct gcgtcgttta ggcatggtgt ggtttatggg
    21301 ccacgacagc agcaagtttc gcattaccga atcggtgttc cgcttcggag ccgatgtgcg
    21361 tgctggcgac gatccccgtg aagcacagcg tcgcctgatt cgtgatggcg aagcaatgcc
    21421 gattgaaaat catctgcaac tcaacgatga aaccgaagag aatcagccag atagcggaga
    21481 ggaagaataa tgattgaacg cggtaaattt cgctcactga cgctgattaa ctggaacggc
    21541 ttttttgccc gaacttttga ccttgacgag ctggtcacga cgctttctgg cggtaacggg
    21601 gcgggtaaat ccaccaccat ggcggcgttc gttacggcgc tgatccccga cctgaccctg
    21661 ctgcatttcc gtaacactac ggaagccggg gccaccagcg gttcgcgcga taaaggtctg
    21721 cacggtaagc tgaaagcggg tgtctgttat tcgatgctcg acaccattaa ctcgcgccac
    21781 cagcgcgtgg tggtcggtgt gcgtctgcaa caggttgccg gacgcgatcg taaagtggat
    21841 atcaagccgt ttgccattca gggactgccg atgtcggtgc agccgacaca gctggtgacc
    21901 gaaaccctga acgaacgcca ggcgcgcgtg ctgccgctta acgagctgaa agacaagctc
    21961 gaggcgatgg aaggcgtgca gtttaaacag ttcaactcca ttactgatta ccactcgctg
    22021 atgttcgatc tgggcatcat cgcgcgtcgt ctgcgctccg catctgaccg tagcaaattc
    22081 tatcgtctga tcgaagcttc gctgtatggc gggatctcca gtgccattac ccgttctctg
    22141 cgcgactacc tgttgccgga aaacagcggc gtgagtaaag cgttccagga catggaagcg
    22201 gcgctgcgtg aaaaccgtat gacgctggaa gcgattcgtg tcacccagtc ggaccgcgac
    22261 ctgtttaagc atctgatcag cgaagccacc aactacgtgg cggcggacta catgcgtcac
    22321 gccaacgagc gccgtgtcca tctcgacaaa gccctggagt ttcgtcgcga gctacatact
    22381 tcgcgtcagc aactggcggc tgagcagtac aaacacgtcg atatggcgcg tgagctggca
    22441 gagcacaacg gtgccgaagg cgatctggaa gcggattatc aggcggccag cgatcacctg
    22501 aacctggtgc aaaccgcact gcgtcagcag gagaagatcg aacgctacga agcggatctc
    22561 gatgagctgc agatccgtct ggaagagcaa aatgaagtgg tggcagaagc catcgaacgc
    22621 caggaagaga atgaggctcg tgcggaagct gccgaactgg aagtggacga gctgaaaagc
    22681 cagcttgctg actaccagca ggcgctggac gtccagcaaa cgcgcgcgat ccagtataac
    22741 caggcgattg ctgcgcttaa tcgtgccaaa gaactgtgcc atctgccgga cttaaccgcc
    22801 gactgcgccg ccgaatggct ggaaaccttc caggcgaaag agctggaagc gactgaaaaa
    22861 atgctctctc ttgagcagaa aatgagcatg gcgcaaaccg cgcacagcca gtttgagcag
    22921 gcttatcagc tggtggtggc aatcaacggc ccgctggcgc gtaacgaggc gtgggatgtc
    22981 gctcgcgaac tattgcgcga aggggtcgat cagcgtcacc tggcagagca ggttcaaccg
    23041 ttgcggatgc gcttaagtga actggaacag cgtctgcgcg agcagcaaga agccgaacgc
    23101 ctgctggcag acttctgcaa acgtcagggc aagaattttg atattgatga actggaagct
    23161 ctgcatcagg agctggaagc gcgcattgcc tctctttccg atagcgtgtc taacgcccgt
    23221 gaagagcgca tggcactgcg ccaggaacag gaacagctgc agtctcgcat tcagagtttg
    23281 atgcagcgtg cgccggtttg gctggcagcg caaaacagtc tcaaccagtt gagcgagcag
    23341 tgcggtgaag agtttacctc cagccaggac gtcaccgaat atctgcaaca gttgctggag
    23401 cgtgagcgag aggcgattgt tgaacgcgat gaagtgggcg cgcgcaaaaa cgctgtcgat
    23461 gaagagatcg aacgtttaag ccagcctggc ggctctgaag atcagcgtct gaacgcgctg
    23521 gcggagcgtt ttggtggtgt gctgctgtca gaaatttatg acgacgttag cctggaagat
    23581 gcgccgtact tctcagcgct gtatggcccg tcacgccacg ccatcgtggt gccagatctg
    23641 tcacaggtaa ctgaacacct ggaaggcttg accgattgcc cggaagatct ctatctgatc
    23701 gaaggagatc cgcagtcatt cgatgacagc gtgttcagcg ttgatgagct ggaaaaagcg
    23761 gtagtggtga aaatcgccga tcgtcagtgg cgttattcac gtttcccgga agtgccgctg
    23821 tttggtcgtg ctgcgcgtga aagccgtatt gaaagcctcc atgccgagcg tgaagtgctt
    23881 tccgaacgct tcgccacgct ctcctttgat gtacagaaaa ctcagcgtct gcatcaggcg
    23941 ttcagccgct ttatcggcag tcatctggcg gttgcgtttg agtctgaccc ggaagcagaa
    24001 atccgtcaac tgaacagccg tcgcgtcgaa ctggagcggg cgttaagtaa tcatgaaaat
    24061 gataaccagc agcagcgtat tcagtttgag caggcgaaag agggcgttac ggcgctgaac
    24121 cgcattctgc cgcgtctcaa cctgttggct gatgacagcc tggcggatcg cgtcgatgaa
    24181 atccgcgaac gtctggatga agcccaggaa gccgcgcgtt ttgttcagca gtttggcaat
    24241 caactggcga aactggaacc gattgtttcg gtattgcaga gcgacccgga acagttcgaa
    24301 cagttaaaag aagattacgc gtactctcag cagatgcagc gcgatgcccg tcagcaggcg
    24361 tttgccctga cggaagtggt gcagcgtcgt gcgcacttta gctattctga ctcggcagaa
    24421 atgcttagcg gtaacagcga tctcaacgaa aaactgcgtg aacgtctgga acaggcggaa
    24481 gcggagcgta cccgcgctcg cgaagcgttg cgcggtcacg cagcgcagtt gagtcagtac
    24541 aaccaggtgc tggcttcgct gaaaagttct tacgacacca aaaaagagct actcaacgat
    24601 ctgcaacgtg aattgcagga tatcggcgtg cgtgctgata gcggggcaga agagcgggcg
    24661 cgtattcgcc gtgacgagct gcatgcgcaa ctgagcaata accgttcacg ccgcaatcaa
    24721 ctggaaaaag cgcttacctt ctgcgaagcg gagatggaca acctgacccg caaactgcgc
    24781 aagctggagc gggattactt tgagatgcgc gagcaggtag tgaccgccaa agcgggctgg
    24841 tgtgcggtga tgcgcatggt gaaagataac ggcgttgagc gccgcttaca ccgtcgtgag
    24901 ctggcttatc tctccgctga tgatttgcgt tccatgtcgg ataaggcgtt aggtgcgctg
    24961 cgtctggcgg tggcggataa cgaacatctg cgcgacgtgc tgcgcatgtc ggaagatccg
    25021 aaacgtccgg agcgtaaaat tcagttcttc gtggcggttt atcagcatct gcgtgaacgt
    25081 attcgtcagg atattattcg taccgatgat ccggtggaag ctatcgaaca gatggagatt
    25141 gaacttagcc gtctgaccga agaattaacc tcccgtgaac agaaactggc gatcagttcc
    25201 cgcagcgtgg cgaacatcat tcgcaaaacc attcagcgcg agcagaaccg tatccgtatg
    25261 ctcaaccagg ggttgcagaa cgtatcgttt ggtcaggtga acagcgtgcg tctcaacgtg
    25321 aacgtgcgtg aaacgcacgc catgctactg gatgtgctct ctgaacagca cgagcagcat
    25381 caggatctgt ttaacagcaa ccgtttgacc ttctcggaag cgctggcgaa actgtatcaa
    25441 cgtcttaacc cgcagattga tatggggcag cgcacgccgc agaccatcgg tgaagaactg
    25501 ctggattacc gcaactatct ggaaatggaa gttgaggtta accgtggttc cgatggctgg
    25561 ctgcgcgcag agtctggtgc attgtcgacc ggtgaggcga ttggtaccgg tatgtcgatt
    25621 ctggtgatgg tggtacaaag ctgggaagat gaatctcgcc gcctgcgcgg taaagatatc
    25681 tctccttgcc gcctgctgtt cctcgatgaa gcagcgcgac tggatgctcg ttctatcgcc
    25741 acgctgtttg aattgtgtga acgtttgcaa atgcagctca tcatcgcagc gccggaaaat
    25801 atcagcccgg agaaaggcac cacctataaa ctggtgcgta aagtcttcca gaataccgaa
    25861 cacgttcacg tcgtcggcct gcgaggattt gcgccgcaac tccctgaaac gcttccagga
    25921 agtgacgaag cgccttctca ggcgagttaa aattaaggcg gcagcaatgc cgccttttct
    25981 ttttccgaaa actccgtttc tgcactaaaa aagtggcaca ttacggcgcg cttttcttta
    26041 aacttcttta cattaggtta tgtaaaaacg tatcggcgtt tatatactga agataagcct
    26101 gatgagtaac aggcttgctc gtcatacttt cgtgagtatt ggcgttgtac aggcaagtcg
    26161 taaaataaca gcctggctat tcagagtatg ataaaaacag ggggcaaggg atgttgctta
    26221 atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg cttggccgta acattcgctc
    26281 cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc tggcgacagc ccggtggctg
    26341 tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac ggcaataatg gcggggatcc
    26401 agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca aatcgaatct caattacccg
    26461 caggttacaa gccggtttat cttaaccagc ttcaactgtt gtatgccgca cgcgatatgc
    26521 aacccatgtg ggaaaaccgt gatgctgtta aagccttcca gcaacagctg gcagaggtgg
    26581 cgattgccgg tttccagccg cagtttaata aatgggtaga gttactgacc gatcctggtg
    26641 ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat gatgggctat ctccatttca
    26701 ttgcaaatat tccggtcaaa ggcactcgct ggctatatag cagtaaacct tatgcacttg
    26761 caacgccgcc gctctcggtg attaaccaat ggcagctggc gctggataaa ggtcaattgc
    26821 ctacgtttgt tgcaggactg gcaccgcagc atccgcaata tgcggcgatg catgaatcgt
    26881 tactggcctt actcagtgac accaaaccgt ggccccaact gaccggcaaa gcaacgttgc
    26941 gcccagggca gtggagtaac gacgtaccgg cgttgcgcga aatattgcaa cgcacaggca
    27001 tgttggacgg ggggccgaaa attactctac ctggcgatga cacgccaact gacgcggtag
    27061 tcagcccatc cgctgttact gttgaaacag cagaaactaa gccgatggac aagcaaacga
    27121 cgtctcgtag taaacctgcg cctgccgttc gtgccgccta cgataatgaa ctggtggaag
    27181 ccgttaaacg ttttcaggca tggcaaggat tgggggcaga tggtgctatt ggcccggcaa
    27241 cacgtgactg gttaaacgta acgcccgccc agcgtgctgg cgtgttggct ctcaacatcc
    27301 agcgattgcg cttgctgcca acagagcttt ctaccgggat catggttaac attccggcct
    27361 attcgctggt ctactatcag aacggcaatc aggtgctgga ttcgcgagtc attgtcggtc
    27421 gccccgatcg caaaacgccg atgatgagca gtgcccttaa caacgtagtg gtaaacccgc
    27481 cgtggaacgt acctccaact ctggcacgca aagatattct gccaaaagtg cgcaacgatc
    27541 cgggatatct cgaaagccat ggctatacgg tgatgcgcgg ctggaacagc agagaagcga
    27601 ttgacccatg gcaggttgac tggtctacaa tcacggcctc gaatttaccg ttccgcttcc
    27661 agcaggctcc aggcccacgg aactcgctgg ggcgctataa attcaatatg ccgagttcag
    27721 aggccattta tttgcatgac acgccgaacc acaatctgtt caagcgtgat acacgcgcat
    27781 tgagctcagg ctgtgtacga gtgaataaag cttccgatct ggcgaatatg ctgttgcagg
    27841 atgcaggctg gaatgacaaa cgtctttctg atgcgctgaa gcagggtgat acacgttacg
    27901 tcaatattcg gcagtcgatt ccggtgaatc tctactacct gacggccttt gttggtgcag
    27961 atggtcgtac ccagtatcgt acagatattt acaattatga tctgcctgcg cgatccagct
    28021 cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat gaagtagttc ggggaaatca
    28081 gttgtcgtaa taattagcat gattgggggc gattctctgc agcccccgtc actgctgggg
    28141 ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg cctcttgtgc gccagaagtg
    28201 catataaacg ataacattga cctgtagact tgattatcat ggacaaattc gacgctaatc
    28261 gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc cgccatcctg ccgacccctg
    28321 cgtttgcaac actctctacc ccacgcccgc gcattttgac actcaataat cttcataccg
    28381 gagagtcaat caaagcggag tttttcgatg gcagaggcta tattcaggaa gaattggcaa
    28441 aacttaacca ttttttccgc gattaccgcg cgaacaaaat aaagtccatc gacccaggat
    28501 tattcgacca gttgtatcgc ctgcaagggt tgttaggcac gcgcaaaccg gtgcaactca
    28561 tttccggtta tcgttctatt gataccaaca atgaactacg cgcccgcagc cgtggagtag
    28621 cgaagaaaag ctatcacact aaaggccagg cgatggattt ccatattgaa ggtatcgcgt
    28681 taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg tggtgtagga tattatccac
    28741 gtagtaactt tgtgcatatt gataccgggc cagcacggca ctggtagtaa ttgcttaacg
    28801 aaacaggggc agtatgaact atcgtattat tccggtcacc gcattctccc agaactgttc
    28861 attaatctgg tgtgaacaaa cccgtctggc cgcactggtc gatcctggcg gcgatgcgga
    28921 aaaaatcaaa caggaagttg atgacagcgg cctgacactg atgcagatcc tgctgacgca
    28981 tggtcatctg gaccacgttg gcgcagcggc ggaactggcg caacattacg gcgtgccggt
    29041 ttttggcccg gaaaaagaag atgagttctg gctgcaaggc ttgcctgcgc aaagtcgtat
    29101 gtttggtctg gaagagtgcc agccgctgac gccagatcgt tggctgaacg aaggcgatac
    29161 catcagcata gggaatgtga ctttacaggt gttacattgc cctgggcata cgccgggtca
    29221 tgtcgtgttt tttgatgatc gggcaaagct gctgatttct ggcgatgtta ttttcaaagg
    29281 cggagtaggg cgcagtgact tcccgcgtgg cgatcataat caactgattt cttcaatcaa
    29341 agataaattg ctgccactgg gggatgacgt gacatttatt ccgggtcacg gaccattatc
    29401 cacacttggt tatgaacgcc tgcataatcc cttcctgcaa gacgaaatgc ccgtctggta
    29461 aggcacataa aaaagcccgc ttttaatgcg ggcctggatt tctggcaaag tgcgctttgt
    29521 ttatgccgga tgcggcacga gcgccttatc cggcctacaa aatcgtgcaa attcaaaata
    29581 ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag gcaatgttgc gtttgtcatc
    29641 agtctcagcc cgcttttcag cgggcttcat tgtttttaat gcttacagca ctgccacaat
    29701 cgcttcgcac agcggagcca tgttatctgg tgtcatcccg gccacattta cgcgaccaga
    29761 agcaaccgca tatacgccaa actcttcgcg cagacgcagc acttgttctt ttgtcaggcc
    29821 actgaaggag aacatgccgt tctgtttgat gataaagctg aagtcgcggt ttgcgccttt
    29881 ttcctgcagc gtattgacga acaactgacg catacgctga atacgctggc gcatatcagt
    29941 cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc aggatggtgg caacaacaga
    30001 agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc gccgctttca tttggctgaa
    30061 tgcgcgatca acggtttcac tgtcggcagc aaccagagta caagcgccaa cacgctcgtt
    30121 gtacaggcca aagtttttag agtaggaact ggcaacaatc agctctttat gcatagccgc
    30181 gaaagcgcgc agtccttcag catcttcttc cagaccacgg gcaaaaccct ggtaagcgaa
    30241 gtcaaacagc ggtaaccagc ctttctcaac tgagagttgt gccagtgtct gccattgttc
    30301 cagcgtaggg tcgataccgg ttgggttatg gcagcagcca tggaacagca ctacgtcgcc
    30361 agcctgagct tcattcaggc tgttaatcag tgcatcgaag tcaagagtgt gattttccgc
    30421 atcataataa gcgtattcac gaacttccag acctgcagag ttaaagacgc tcttatggtt
    30481 cggccagctt gggttgctca cccacactcg cttaacgctg gtattttttg ccaggaaatc
    30541 ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt gccgtgcgag cacgtttgtc
    30601 attgatcagg gcgctacctt taccaaacag cagttcctga gtgcagcgac caaattcagg
    30661 gatgccgtca atgccgaggt aatttttggt ggtttcattt tcgagcagat actgttcagc
    30721 ctttttcacg ctggtcagta ccggggtttt gcccgtctca tctttataga caccaatccc
    30781 gaggttaatt ttgccgggac gttcatcggc acgaaacaga tcggccaggc ccagaatcgg
    30841 gtcggcagga gcggcggtaa tgttctcaaa catgacgagg ttccattatg gttacagaag
    30901 ggaagtccgc tatcagggta acgggagatt tacaaaattc caactattac tgatgaaaac
    30961 gcaggctgtt tttgcaagac gtgagattgc tctggaaggt ataaaaaaaa caggaccaaa
    31021 gtcctgtttt ttcggcattt aacaaagagg tatgctatta gaactggtaa acgataccca
    31081 cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc agaatcgatc tggttgatga
    31141 tgtagtcaac ataggtggac atgtttttgt tgaagtagta ggttgcgccc acttcaaagt
    31201 agttcaccag atcaacatca ccgatacctt ctacgtcttt cgctttagat ttggtgtaag
    31261 cgatggacgg acgcagaccg aaatcgaact ggtattgcgc aactaacaga acgtcttgcg
    31321 ttttgttggc gaagccgctg gtgtttgtaa atttattagt gatcggcgta gcgttacggg
    31381 tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc gtacttcaga ccagtagccc
    31441 actgttcagc ttttttaccg ttgccaagag gttgagcttc ttgcaggttg gtacggtcag
    31501 ctgcaccata agcaccaacg ataccaaagc cttcgtattc gtagctgata gaaccgccaa
    31561 caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt tttacccagg tactgaacag
    31621 cgaagttcag gccatcaacc agaccaaaga agttggagtt acgataggta gcaacgccgc
    31681 caacacgacc aacgaagaag tcatcgctgt atgcagtatc accaccaaat tctggcagca
    31741 tatcggtgta acccagtgca tcataaacca caccgtagtt acggccgtaa tcgaaagaac
    31801 caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt tttgttacca gtttgagcgt
    31861 cagcgccttc agagttgtta ccctggaagt tatattccca ctgaccataa ccggtcagat
    31921 cggaattgat ttgagtttcc cctttaaaac caagacgggc ataggtcatg tcgccattgc
    31981 caccgtaact gttttcaccg ttacccttgg aaaaataatg cagaccaaca gctttaccgt
    32041 acagatctac tttgttgcca tctttgttat agatttctgc agcgtttgca gtacctgcta
    32101 ctaacagagc agggacgatc actgccagaa tattgcgctt catcattatt tattaccctc
    32161 atggtttttt ttatgacacc tgccactgcc gtcaataagt tctgtcaata aaaatttacg
    32221 gaactattga tgagagtttg gtgtctttat gtgtctgcag gcatctttcc attcaaacta
    32281 acgtttc
//
LOCUS       DAZGVQ010000041        32270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000041 DAZGVQ010000000
VERSION     DAZGVQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.041"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            405..1976
                     /gene="garD"
                     /locus_tag="ACHLAG_001855"
     CDS             405..1976
                     /gene="garD"
                     /locus_tag="ACHLAG_001855"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFP0655099.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QLERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            2125..2460
                     /gene="prlF"
                     /locus_tag="ACHLAG_001856"
     CDS             2125..2460
                     /gene="prlF"
                     /locus_tag="ACHLAG_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFP0655100.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2460..2924
                     /gene="yhaV"
                     /locus_tag="ACHLAG_001857"
     CDS             2460..2924
                     /gene="yhaV"
                     /locus_tag="ACHLAG_001857"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFP0655101.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2979..3788)
                     /gene="agaR"
                     /locus_tag="ACHLAG_001858"
     CDS             complement(2979..3788)
                     /gene="agaR"
                     /locus_tag="ACHLAG_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFP0655102.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            4037..5317
                     /gene="kbaZ"
                     /locus_tag="ACHLAG_001859"
     CDS             4037..5317
                     /gene="kbaZ"
                     /locus_tag="ACHLAG_001859"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFP0655103.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYYTACEGQ"
     gene            5340..5813
                     /gene="agaV"
                     /locus_tag="ACHLAG_001860"
     CDS             5340..5813
                     /gene="agaV"
                     /locus_tag="ACHLAG_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFP0655104.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5824..6603
                     /gene="agaW"
                     /locus_tag="ACHLAG_001861"
     CDS             5824..6603
                     /gene="agaW"
                     /locus_tag="ACHLAG_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFP0655105.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6593..7471
                     /gene="agaE"
                     /locus_tag="ACHLAG_001862"
     CDS             6593..7471
                     /gene="agaE"
                     /locus_tag="ACHLAG_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFP0655106.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7489..7923
                     /gene="agaF"
                     /locus_tag="ACHLAG_001863"
     CDS             7489..7923
                     /gene="agaF"
                     /locus_tag="ACHLAG_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFP0655107.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7920..9053
                     /gene="nagA"
                     /locus_tag="ACHLAG_001864"
     CDS             7920..9053
                     /gene="nagA"
                     /locus_tag="ACHLAG_001864"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP0655108.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNSIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            9404..10558
                     /gene="agaS"
                     /locus_tag="ACHLAG_001865"
     CDS             9404..10558
                     /gene="agaS"
                     /locus_tag="ACHLAG_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFP0655109.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10571..11431
                     /gene="kbaY"
                     /locus_tag="ACHLAG_001866"
     CDS             10571..11431
                     /gene="kbaY"
                     /locus_tag="ACHLAG_001866"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFP0655110.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11598..12074
                     /gene="agaB"
                     /locus_tag="ACHLAG_001867"
     CDS             11598..12074
                     /gene="agaB"
                     /locus_tag="ACHLAG_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFP0655111.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            12113..12916
                     /gene="agaC"
                     /locus_tag="ACHLAG_001868"
     CDS             12113..12916
                     /gene="agaC"
                     /locus_tag="ACHLAG_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFP0655112.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12906..13697
                     /gene="agaD"
                     /locus_tag="ACHLAG_001869"
     CDS             12906..13697
                     /gene="agaD"
                     /locus_tag="ACHLAG_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFP0655113.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13698..14453
                     /gene="agaI"
                     /locus_tag="ACHLAG_001870"
     CDS             13698..14453
                     /gene="agaI"
                     /locus_tag="ACHLAG_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFP0655114.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
     gene            14853..15437
                     /gene="yraH"
                     /locus_tag="ACHLAG_001871"
     CDS             14853..15437
                     /gene="yraH"
                     /locus_tag="ACHLAG_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0655115.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            15517..16212
                     /gene="yraI"
                     /locus_tag="ACHLAG_001872"
     CDS             15517..16212
                     /gene="yraI"
                     /locus_tag="ACHLAG_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0655116.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            16242..18758
                     /gene="yraJ"
                     /locus_tag="ACHLAG_001873"
     CDS             16242..18758
                     /gene="yraJ"
                     /locus_tag="ACHLAG_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0655117.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFNEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            18769..19860
                     /gene="yraK"
                     /locus_tag="ACHLAG_001874"
     CDS             18769..19860
                     /gene="yraK"
                     /locus_tag="ACHLAG_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0655118.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(19903..20763)
                     /gene="rsmI"
                     /locus_tag="ACHLAG_001875"
     CDS             complement(19903..20763)
                     /gene="rsmI"
                     /locus_tag="ACHLAG_001875"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFP0655119.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQKGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            20827..22863
                     /gene="lpoA"
                     /locus_tag="ACHLAG_001876"
     CDS             20827..22863
                     /gene="lpoA"
                     /locus_tag="ACHLAG_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFP0655120.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            22821..23216
                     /gene="yraN"
                     /locus_tag="ACHLAG_001877"
     CDS             22821..23216
                     /gene="yraN"
                     /locus_tag="ACHLAG_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFP0655121.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            23236..23826
                     /gene="diaA"
                     /locus_tag="ACHLAG_001878"
     CDS             23236..23826
                     /gene="diaA"
                     /locus_tag="ACHLAG_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFP0655122.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            23836..24411
                     /gene="dolP"
                     /locus_tag="ACHLAG_001879"
     CDS             23836..24411
                     /gene="dolP"
                     /locus_tag="ACHLAG_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFP0655123.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(24510..25517)
                     /gene="yhbW"
                     /locus_tag="ACHLAG_001880"
     CDS             complement(24510..25517)
                     /gene="yhbW"
                     /locus_tag="ACHLAG_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFP0655124.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(25723..26601)
                     /gene="ubiV"
                     /locus_tag="ACHLAG_001881"
     CDS             complement(25723..26601)
                     /gene="ubiV"
                     /locus_tag="ACHLAG_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFP0655125.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGISN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            complement(26610..27605)
                     /gene="ubiU"
                     /locus_tag="ACHLAG_001882"
     CDS             complement(26610..27605)
                     /gene="ubiU"
                     /locus_tag="ACHLAG_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFP0655126.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            27814..28338
                     /gene="ubiT"
                     /locus_tag="ACHLAG_001883"
     CDS             27814..28338
                     /gene="ubiT"
                     /locus_tag="ACHLAG_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFP0655127.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            28332..28835
                     /gene="yhbS"
                     /locus_tag="ACHLAG_001884"
     CDS             28332..28835
                     /gene="yhbS"
                     /locus_tag="ACHLAG_001884"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0655128.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(28822..29124)
                     /gene="yhbQ"
                     /locus_tag="ACHLAG_001885"
     CDS             complement(28822..29124)
                     /gene="yhbQ"
                     /locus_tag="ACHLAG_001885"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFP0655129.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAESAGFAELLSSLQTPEIKSD"
     gene            29175..29618
                     /gene="yhbP"
                     /locus_tag="ACHLAG_001886"
     CDS             29175..29618
                     /gene="yhbP"
                     /locus_tag="ACHLAG_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFP0655130.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            complement(29598..30116)
                     /gene="yhbO"
                     /locus_tag="ACHLAG_001887"
     CDS             complement(29598..30116)
                     /gene="yhbO"
                     /locus_tag="ACHLAG_001887"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFP0655131.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            30244..30879
                     /gene="yraR"
                     /locus_tag="ACHLAG_001888"
     CDS             30244..30879
                     /gene="yraR"
                     /locus_tag="ACHLAG_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFP0655132.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            30952..31992
                     /gene="yraQ"
                     /locus_tag="ACHLAG_001889"
     CDS             30952..31992
                     /gene="yraQ"
                     /locus_tag="ACHLAG_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP0655133.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(32164..>32270)
                     /locus_tag="ACHLAG_001890"
     CDS             complement(32164..>32270)
                     /locus_tag="ACHLAG_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP0655134.1"
                     /translation="VILGAMMRKLAQVAYGVLKSGVPFDASRHNPVAA"
BASE COUNT         7903 a         8117 c         8418 g         7832 t
ORIGIN      
        1 ctttttttcg tcaacggtgt ccagaatcat tttatttacc tcgggtactt atgctgattt
       61 ttattattat ggggaaggtg ttatttatga gtttcattta tgccgtaacg gcaatgaact
      121 cgggaattag tataagcagc gcgagaataa taatcattgt gcaaatgcta atttaattaa
      181 tgctatttaa atattatttt gagcatatgc acataaggtt gcgcgctaaa gcacagattt
      241 gcgctttacc ttgccgggcg gcactgcaat ccctgaaatg attgacattg atcacatttc
      301 tgcgtttaaa ctcctgacat tcttatttca cccaatgaag tcatttattt ttaaatgaga
      361 ccaggtcctc attttaataa cccctggctg gagaatatag tacaatggcc aacatcgaaa
      421 tcagacaaga aacgccaact gcgttttata taaaagttca cgacacagat aatgtggcaa
      481 ttattgttaa tgataatggc ctgaaagcag gaacgcgttt tccggatgga ctggaattaa
      541 ttgaacatat tccccagggg cataaagtcg cattgctgga cattccggct aatggtgaaa
      601 ttattcgtta tggtgaagtg attggttacg ccgtgcgtgc aatcccacgc ggaagctgga
      661 tcgacgaatc aatggttgta ctaccggaag cgccgccgtt acacacgctg ccactggcaa
      721 ccaaagtccc ggccccctta ccgccgctgg aaggatacac ctttgagggc tatcgcaatg
      781 ccgatggcag cgtgggcacc aaaaacctgc tcggcatcac caccagcgtc cactgtgtgg
      841 caggcgtggt ggactatgta gtaaaaatca ttgaacgcga tctgctaccg aaatacccga
      901 acgtcgatgg cgtggtgggg ctgaatcatt tgtacggctg tggcgtggcg attaacgcac
      961 cggcggcagt tgtgcctatt cgtaccatcc acaatatttc gctgaaccct aactttggcg
     1021 gcgaagtaat ggtgattggt ctgggttgtg aaaagttgca gcttgagcgc ctgctggtcg
     1081 gcacggatga tgtgcaagct attccagtag aaagcgccag cattgtcagt ttgcaggatg
     1141 aaaagcatgt cggttttcag tccatggtcg aggatatttt gcaggtcgcc gaacgccatc
     1201 tacaaaaact gaatcaacgt cagcgagaaa cctgtccggc ttcagaactg gttgtcggca
     1261 tgcagtgcgg tggcagcgat gccttttctg gcgtaacggc aaacccggcg gttggctatg
     1321 cgtctgatct actggtgcgc tgcggcgcaa cggtgatgtt ctcagaagtc acggaagtgc
     1381 gtgacgcgat ccatctgctg acaccacgcg cagtgaacga agaggtcggc aaacggctgc
     1441 tggaggagat ggagtggtac gataactatc tcaatatggg aaaaaccgac cgcagcgcca
     1501 acccttcgcc gggcaacaag aaaggcggtc tggcaaacgt ggtagagaag gcactcggct
     1561 ccattgctaa atcgggtaaa agcgcaattg ttgaagtgct gtcgcccggt caacgcccga
     1621 ctaaacgcgg attaatttac gccgcgacgc cagccagcga ttttgtctgt ggcacgcaac
     1681 aggtggcttc gggtatcacc gtgcaagtgt ttacgaccgg tcgtggtacg ccgtacggcc
     1741 tgatggcggt acccgtcatt aaaatggcga cccgcaccga gctggcgaac cgctggtttg
     1801 atttaatgga tattaacgcg ggcactatcg ccaccggcga agaaaccatt gaagaggtgg
     1861 gctggaagtt gttccacttt attctcgacg tcgccagcgg gaagaagaaa accttctcgg
     1921 atcaatgggg gctgcataac cagctggcag tgtttaaccc ggcaccggtg acctgatttc
     1981 ttttggaacg tccctcgcaa aacatggcct tagtgccatg tttttattgt ttaaagcccc
     2041 cacgtccatt aataatgcat ttgcattacc ttagttcaag cttataattt gagcagaaaa
     2101 caggctgtaa aaggacagtg aatcatgccc gctaatgctc gctctcacgc tgtactgacc
     2161 actgaatcaa aggtcacgat acgcggacaa acaactatcc ccgcgccagt gcgtgaggcc
     2221 ttaaaactga agccaggcca ggacagcatt cattacgaaa ttctgcctgg tgggcaagtc
     2281 tttatgtgcc gactgggaga tgaacaggag gatcatacta tgaatgcatt tttgcgtttt
     2341 ctggatgcag atatccagaa caacccgcaa aaaactcgtc cattcaacat tcaacaagga
     2401 aagaaacttg tcgctggcat ggacgtcaac attgatgatg agattggcga cgacgaataa
     2461 tggattttcc acaaagggtt aatggttggg cgctatatgc tcatccctgt tttcaggaaa
     2521 cctacgacgc tttagttgcc gaagtcgaga cattaaaggg aaaagatcct gaaaattatc
     2581 agagaaaagc cgccacaaag ttattggcgg tagtccataa agtgattgag gagcatatca
     2641 cggtcaatcc atcatcaccg gcattccgtc atggcaagtc gttaggctct gggaaaaata
     2701 aagactggtc acgggtaaaa tttggtgctg gtcgttatcg tctcttcttt cgttatagtg
     2761 aaaaagagaa agtcatcatt ctgggatgga tgaacgatga aaacactctg cgcacctacg
     2821 gtaaaaaaac agatgcctat accgtattca gcaaaatgtt aaaaagagga catcctcctg
     2881 ccgactggga aagcctcacc caagagacag aagaaaacca ttgatggtgt tcacaatgcc
     2941 cgccgaacgt attcgtgttg accagcgggc accctttctc actccccgac cagaatcact
     3001 tcaaccccag cctttcgcag tccttccaga ctatccgcag gaatgccttc atcaacaatg
     3061 atcatgtcga tacgttgagt atcaatgatc ttatgtaaac tggaacgatt gaatttactg
     3121 gaatcggtga ccacgatgat ccgttccgca acttcgcaca tccgacggtt taaacgggct
     3181 tcatcttcat tatgtgtgct gacgccgcgc tccagatcga tcgcatctac accaagaaac
     3241 agcatatcga agtggtaatt ttgcagcgat tgctcagcct gatcgccgta aaaagattgc
     3301 gactgacggc gcaaatgccc gccggtcatc agcagctcaa cgccttccgc ttccagcaac
     3361 gcattagcca cgttcatacc gttggtcatc gcaattacgt cagtgtgctt acgcatcaga
     3421 cgagcaatct caaaagtggt agtcccggaa tcgaggatca cccgatgacc aggctgaatc
     3481 aactcaacgg cagctttcgc aacgctgcgt ttcatcgcgg tgttcagcgc gcttttatct
     3541 tccactgatg gctcgactga cggcgtcgtg ctatcgcaga tcaacgcgcc accataggca
     3601 cgcacagcga tcccctgctt ttccagaaac gccagatcat tgcggatcgt cacagtagat
     3661 acgccataca atgccgacag atcgttaacc tgcacactcc cttgctgtcg cagacgctga
     3721 atgatctgtt ctcgtcgctc gctggtgcca gtcactcgct tctcacctga agcgtcggta
     3781 ttactcatag taagtccttt cgtaaaactt tcgtttcatt tcgttttgcc tattaacgcc
     3841 tttctattaa gcaaatgcaa gcccaccttg cccattgacg caagctactc tcgtttcagt
     3901 gactttcatt atgtttcttt tgtgaatcag atcagaaaac tattatcttt cgttttattt
     3961 ttatctcacc atgacgcagt atcaactgaa acaaaacgaa agattaatat cgcagtaatc
     4021 tgaactggag aggaaagtga aacatctgac agaaatggtg agacagcaca aagcgggcaa
     4081 aacaaatgga atttatgccg tttgttccgc acatccgctg gtgctggagg ctgcaatccg
     4141 ctacgccagt gcaaaccaaa cgccgttact gattgaagca acctccaatc aggtagacca
     4201 gttcggcggt tataccggaa tgacgcccgc cgattttcgc ggctttgttt gtcagctcgc
     4261 cgactcgttg aatttcccgc aggatgcgtt gattctgggt ggtgaccatc tggggccaaa
     4321 ccgctggcaa aacctgccgg ccgctcaggc aatggccaat gccgatgatt tgattaaaag
     4381 ctacgttgcg gcaggattca aaaaaatcca ccttgattgc agcatgtcct gtcaggacga
     4441 tccgattccc ttaactgatg acatcgtggc tgaacgcgcc gcccgtctgg cgaaagtggc
     4501 ggaagaaacc tgtcttgaac actttggcga agccgatctg gagtatgtca ttggtaccga
     4561 agtgccggta cctggcggcg cgcatgaaac cttaagcgag ctggcggtca ccacgccgga
     4621 tgccgcccgc gccacgctgg aagcccatcg tcacgccttt gaaaagcaag gtttgaatgc
     4681 catctggcca cgcatcattg ccctggtggt tcaacccggc gtcgaattcg atcacaccaa
     4741 cgttattgat tatcagcccg ccaaagcgag cgccttaagc cagatggtcg aaaactacga
     4801 aacgctgatt ttcgaagcgc actctaccga ttatcaaacg ccgcaatcgc tgcgccagct
     4861 ggtgattgac cactttgcca ttctgaaagt tggcccagcg ctgaccttcg ccctgcgtga
     4921 agctctgttc tctctggcgg cgattgaaga agaactggtg ccagcgaaag cctgttctgg
     4981 tctgcgtcag gtgctggaag acgtgatgct cgaccgcccg gaatactggc aaagccacta
     5041 ccacggtgac ggcaacgcgc gtcgtctggc gcgtggttat agctactcgg atcgcgtgcg
     5101 ctattactgg ccggacagcc agattgatga cgctttcgct catctggttc gtaatctggc
     5161 ggattcacca attccgctac cgctcatcag ccagtatctg ccgctgcaat acgtgaaagt
     5221 tcgctccggc gagctgcaac cgacgccacg ggaactcatt atcaaccata ttcaggacat
     5281 cctggcgcag tactacacag cctgtgaagg ccaataagca aaacaaagag gaacacgcta
     5341 tgccaaatat tgttttaagc cggattgatg aacgcttgat tcacggtcag gtcggcgttc
     5401 agtgggtcgg atttgcgggg gcaaatctgg tgctggtagc caacgatgag gttgccgaag
     5461 atccggtaca acaaaacctg atggaaatgg tactggcaga agggatcgcc gtacgtttct
     5521 ggacgctgca aaaagttatc gacaacattc atcgcgccgc cgatcgacag aaaatcctgc
     5581 tggtttgtaa aacacccgcc gatttcctga cgctggtgaa aggtggcgtt ccggtgaatc
     5641 gcattaacgt tggcaatatg cactacgcca atggcaaaca acaaatcgcc aaaacggttt
     5701 ctgtggatgc gggcgatatc gcagcattta acgacctgaa agccgctggg gtggaatgct
     5761 tcgttcaggg cgtcccgaca gagcctgctg tggacctctt taaattactt tgagggattc
     5821 atcatggaaa tcagcctgtt gcaggcattt gcgttgggca ttatcgcctt tatcgctggc
     5881 ctggatatgt ttaacggcct aacacatatg caccgcccgg tggtcctcgg cccgttggtc
     5941 gggctggtac ttggcgatct gcataccgga attttaaccg gcggtacgct ggaactggtg
     6001 tggatggggc tggccccgct ggcgggcgca cagccgccta acgtgattat cggtactatc
     6061 gtcggcacgg cgtttgccat tactactggc gtgaaacccg atgtcgcggt cggtgtcgcc
     6121 gtacctttcg ctgtcgcagt acagatgggg attaccttcc tgttctcggt gatgtctggc
     6181 gtgatgtctc gctgcgaccg gatggcggag aacgccgata ctcgcggcat tgaacgcgtg
     6241 aactatctgg cactgctggc actcggcact ttctattttc tctgcgcttt cctgcctatc
     6301 tacttcggcg cggaacatgc aaaaaccatc attgatgtcc tgccgcaacg attaatcgac
     6361 ggcctcggcg tcgcgggcgg catcatgcca gcaatcggtt ttgccgtgct gctgaaaatc
     6421 atgatgaaaa acgtctacat cccctacttc atcctgggct ttgttgccgc agcctggctc
     6481 aagttaccgg tgctggctat tgctgccgct gcgctggcga tggcgctgat cgacctgctg
     6541 cgtaaatctc ctgaaccgac tcaacctgcg gcacagaaag aggaatttga agatggcatc
     6601 taatcaaacc accctgccga acgtcagtga aaacgaagaa acattgctga ctggcgtcaa
     6661 tgaaaacgtg tatgaagatc agagcattgg cgcggagctg acgaaaaaag atatcaaccg
     6721 cgtcgcctgg cgttccatgc tgttacaagc gtcttttaac tacgaacgta tgcaggcttc
     6781 tggctggttg tacggtctgc tgcctgctct gaaaaagatc cacactaata aacgcgacct
     6841 ggcgcgcgcc atgaaggggc atatgggttt cttcaatacc catccgtttc tggtgacatt
     6901 tgttatcggc attatccttg cgatggagcg ttctaagcag gacgttaaca gtattcagag
     6961 caccaaaatt gccgtcggtg cgccgctcgg cgggattggt gatgcgatgt tctggctgac
     7021 gctactgccg atttgtggcg ggataggcgc aagccttgcg ctacaaggct ctattcttgg
     7081 cgcagtcgta tttattgtgc tgttcaacgt ggtacacctg gggctgcgtt ttggtctggc
     7141 gcattatgct taccgcatgg gcgtggcggc gattccactc attaaagcga ataccaaaaa
     7201 agtcggccat gctgcgtcta tcgttgggat gacggtaatc ggcgcgctgg tggcaaccta
     7261 tgttcgttta agcaccacgc tggaaatcac tgcgggcgac gcagtggtta agttacagac
     7321 tgatgttatt gacaaactga tgcctgcctt cttaccgctg gtctacacct tgaccatgtt
     7381 ctggctggta cgccgcggct ggagtccgct gcgtctgatt gcagttaccg tggttctcgg
     7441 catcgtcggt aaattctgcc atttccttta aatacaatga ggtattagat gttaagtatt
     7501 attttgacag ggcatggcgg gtttgccagc ggcatggaaa aagcaatgaa gcaaattctc
     7561 ggtgagcaat cgcagtttat cgccatcgat tttccggaaa cctcaagcac cgcgctgctt
     7621 acatcgcagc ttgaagaggc catcgcccaa ctggattgtg aagacggcat tgtttttctg
     7681 acggatttgc tgggcggcac gccgtttcgc gtagcttcaa cgctggcgat gcaaaaaccg
     7741 ggctgtgaag tgatcaccgg caccaatttg caactgctac tggagatggt gctggagcgc
     7801 gaagggttaa gcggtgaaga attccgtgta caggcgctgg agtgtggaca ccgagggctg
     7861 accagtctgg tggacgagtt aggccgctgc catgaagaat gtccggtcga ggaaggaata
     7921 tgacacacgt tctgcgcgcc agaaggctgc tgactgaaga gggatggctc gatgaccatc
     7981 agttgcgtat tgctgacggt gtcatcgcag caatcgaacc gattccagtg ggcgtgactg
     8041 aacgcgatgc ggaactgctc tgccccgctt acatcgacac ccatgtacac ggtggtgcgg
     8101 gcgttgatgt aatggatgac gcgccggatg tactcgacaa gctggcaatg cacaaggcac
     8161 gcgaaggtgt cggcagttgg ttgccgacta ccgtaaccgc gccgcttaac agcattcatg
     8221 cggcgctgaa acgtattgct caacgttgcc aacgcggcgg acctggtgcg caagtgctgg
     8281 ggagttatct cgaaggaccg tacttcacgc cgcagaataa aggcgcgcat ccgccggaat
     8341 tgtttcgcga gcttgaaatt gccgagctgg atcaattgat tgccgtttct cagcacacct
     8401 tacgcgtggt agcgctggca ccggaaaaag agggggcatt gcaggccatc cgccatctta
     8461 aacagcaaaa tgtacgagtg atgctggggc atagcgcggc gacctggcaa caaactcgcg
     8521 ccgcgtttga tgctggtgcc gacggcctgg tgcattgcta taacgggatg acaggtttac
     8581 atcaccgcga accgggaatg gttggcgcgg gattaacgga caagcgcgcc tggctggaac
     8641 tgatagccga tggtcatcat gtgcatccgg cggcgatgtc gctgtgttgt tgctgtgcaa
     8701 aagagagaat cgtgctgatc accgacgcga tgcaggcagc cgggatgccg gatggtcgct
     8761 atacgttatg tggcgaagaa gtgcagatgc acggtggcgt tgtccgtacc gcgtccggtg
     8821 ggctggcggg cagtacgctg tctgttgatg cggcagtgcg caacatggtc gagttgacgg
     8881 gcgtaacgcc tgcggaagcc attcatatgg catcgctgca tccggcgcga atgctgggtg
     8941 ttgatggtgt tctgggatcg cttaaaccgg gcaaacgcgc cagcatcgtt gcgctggata
     9001 gcgggctgca tgtgcaacaa atctggattc agagtcaatt agcttcgttt tgatattttg
     9061 ctcctttatt gggccttcac ttcccccgta aggcctttct ttttctttcg ttttgatctg
     9121 tgcagcggtg tcggatgcga cgctaacgcg tcttatgcga cctacggtac gccgcatcca
     9181 gcaatcggtg aacatagcga cgtcattacg caattcttta atcttccctt gtcttttctt
     9241 tcaatgatca cagatttcgt tttatttcct ttttctccat tgaactttcg gtttcttttc
     9301 tatagatttt aatcaacgaa agacatcacc aagtgaaatg aaacgaaagg caagtgaaag
     9361 cgacaacgcc cgacgtcaag ttcatcagac taaggattga gttatgccag aaaattacac
     9421 ccctgctgcc gccgcaaccg gtacatggac tgaagaagag atccgccatc agcctcgcgc
     9481 atggatccgt tcactcacca acatcgacgc gctacgttct gcgctcaata acttccttga
     9541 accgttactg cgcaaagaga atctgcggat catcctgacc ggagccggaa cctcggcatt
     9601 tatcggtgac atcatcgcgc cgtggctcgc cagccatacc ggtaaaaact tcagcgccgt
     9661 accgaccacc gatctggtca ccaatccgat ggactacctg aacccagctc atccgctgct
     9721 gttgatctcc ttcggtcgat ccggcaacag cccggaaagc gtcgctgccg tggaactggc
     9781 aaatcaattt gtaccagaat gctatcacct gccgatcacc tgcaacgaag cgggcgctct
     9841 ttaccaaaac gcgatcaaca gcgataacgc gtttgccctg ctgatgcccg cagaaacgca
     9901 cgatcgcggc tttgcgatga ccagcagcat taccaccatg atggccagct gcctcgcggt
     9961 tttcgcacct gagacgatca acagccaaac cttccgcgac gtggcggatc gttgccaggc
    10021 gatcctgacc tcactgggcg atttcagcga aggtgtgttt ggttacgcac cgtggaaacg
    10081 gatcgtttat ctcggcagcg gtggcttaca gggcgcagca cgcgagtcgg cgctgaaagt
    10141 gctggaactg actgcgggta aactggcggc cttctatgat tccccgaccg gattccgtca
    10201 tggcccgaaa tcactggtcg ataacgaaac gctggtggtg gtatttgtct caagccaccc
    10261 ttacacccgt cagtatgatc ttgatctgct ggctgaactc cgccgtgaca accaggcaat
    10321 gcgcgtaatc gccatcgccg cggaaagcac cgacatcgtc gctgccggtc cacatattat
    10381 cctgccgccg tcacgtcact ttatcgacgt tgagcaggca ttttgcttcc tgatgtacgc
    10441 ccagacgttt gcactgatgc agtcgctgca catgggcaat acgccggata ccccatcagc
    10501 cagcggcacc gttaaccgcg tggtgcaagg cgtaatcatt catccgtggc aggcataaga
    10561 ggatcgcatt atgagcatta tctccactaa atatctgtta caggacgccc aggccaatgg
    10621 ctacgcggtg cctgctttta acattcataa cgccgagacg atccaggcga tcctcgaagt
    10681 gtgcagtgaa atgcgatcgc cggtgatcct cgccggaacg ccggggacct ttaaacatat
    10741 cgcgctggaa gagatctacg ccctgtgcag cgcctattcc acaacctaca acatgccact
    10801 ggcgctgcat ctcgaccacc acgaatcgct ggatgatatt cgccgtaaag tccacgcagg
    10861 tgtgcgcagc gcaatgatcg acggcagcca cttcccgttt gccgagaacg tgaagctggt
    10921 gaaatcggtt gttgacttct gccactcaca ggattgcagc gtggaagcag aactgggccg
    10981 cctgggcggt gttgaagatg acatgagcgt tgacgccgaa agtgcattcc tgaccgatcc
    11041 acaagaagct aaacgctttg tcgaactgac tggcgtcgac agcctggcgg tagcgattgg
    11101 tacggcgcac ggcttataca gcaaaacgcc gaagattgat ttccagcggc tggcggaaat
    11161 tcgtgaagtg gtggatgttc ctctggtgct acatggtgcc agcgatgttc cggatgaatt
    11221 tgtccgtcgc actattgaac ttggcgtcac aaaagtgaac gttgccacag aattaaaaat
    11281 agccttcgct ggcgcggtta aagcctggtt tgcggaaaat ccgcagggta atgatcctcg
    11341 ttattatatg cgcgtcggaa tggatgcgat gaaagaagtt gtcagaaata aaattaatgt
    11401 ctgtggttca gcgaatcgaa tttcagcata atcattagtt ttttatgatt tatcccaatg
    11461 tacttcccga ttaatatcgg ggagtgcctt aatggaaaag gagatgacta aaccttaata
    11521 aatacatcag tacaatatcg caacaataat atattaaaaa aaattatatt attcaacttt
    11581 atggtgagga ttacacaatg agcagtccaa atattctctt aacccgtatt gataaccgtc
    11641 tggttcacgg tcaggttggc gtgacctgga catccaccat cggtgcgaat ctgctggtag
    11701 tcgtggatga tgttgtcgct aacgatgata ttcaacagaa attaatgggt attaccgcag
    11761 aaacctacgg ctttggcatt cgtttcttta ctatcgaaaa aaccattaac gtcatcggca
    11821 aagctgcacc gcatcagaag atcttcctga tttgccgtac gccacaaacg gtacgtaaat
    11881 tggtagaagg tggcattgac ctgaaagata tcaacgtcgg caatatgcat ttctcggaag
    11941 ggaaaaagca aatcagcagt aaagtttatg tcgatgacca ggatctcgcg gacttacgtt
    12001 ttattaaaca acgtggcgtg aatgttttca ttcaggacgt ccctggcgat caaaaagaac
    12061 aaatccctga ctaaatctaa aatcgcctta atattggttt gaggtaataa aaatgcatga
    12121 aataacccta cttcagggat tatccctggc ggcgttagtt ttcgttctgg ggattgattt
    12181 ttggctggaa gccttatttt tattccgccc gataatcgtt tgtaccctaa ctggcgctat
    12241 tctcggtgat attcagactg gcttaattac cggtggtctg acagagttgg ctttcgccgg
    12301 attaacccct gcaggtggtg ttcagccgcc caacccgatt atggcgggtc tgatgaccac
    12361 cgtcattgca tggtctacgg gcgttgatgc caaaacagca attggtcttg gcctgccgtt
    12421 tagtttgtta atgcagtacg tcattctgtt cttctattcc gctttctcat tatttatgac
    12481 caaagccgat aaatgcgcga aagaggcgga tacggcagcg ttttcccggc ttaactggac
    12541 aacgatgctc atcgtcgctt cagcgtatgc ggtgattgct ttcctctgta cttacctggc
    12601 acaaggggcg atgcaggcgc tggtgaaagc gatgcccgcc tggttgaccc acggctttga
    12661 agtggctggc ggtattctgc ctgccgttgg ttttggcttg ctgctgcgcg taatgttcaa
    12721 agcgcaatat atcccttacc tgatcgccgg tttcctgttt gtttgctaca tccaggtcag
    12781 caacctgttg ccggttgccg tactgggcgc aggctttgcg gtgtatgagt ttttcaatgc
    12841 gaaatcccgg cagcaagcgc aaccgcagcc cgttgccagt aaaaatgaag aagaggacta
    12901 cagcaatggg atctgaaatc agtaaaaaag atatcacccg tctgggcttt cgttcgtcgc
    12961 tgctgcaagc gagctttaac tacgaaagga tgcaggcggg cggttttacc tgggcgatgt
    13021 tgccgatcct gaaaaagatt tataaggacg acaaaccggg cttaagcgcg gcgatgaaag
    13081 ataacctcga atttattaat acccacccga atctggtcgg attcctgatg gggttattaa
    13141 tttcgatgga agaaaaagga gaaaaccgcg acaccattaa aggcctcaaa gtggcactgt
    13201 ttggcccaat cgccgggatt ggcgatgcga ttttctggtt tactttgttg ccgattatgg
    13261 cgggaatttg ctcatcattt gccagccagg gaaacctgct ggggccgatt ctatttttcg
    13321 ccgtttacct gcttatcttt ttcctgcgcg tcggctggac ccacgtcggt tattcagtcg
    13381 gcgtgaaggc gatcgataaa gtgcgagaga actcgcagat gattgctcgc tcggcaacca
    13441 tcctcggaat cacggtaatc ggcgggctga tcgcttcgta tgtgcatatt aacgtggtga
    13501 catcgtttgc catcgacagt acccacagcg tcgcgctgca acaggatttc ttcgataaag
    13561 tcttcccgaa cattttaccg atggcctaca ccctgctgat gtattacttc ctgcgggtga
    13621 aaaaagcgca tccggtgctg ttaatcggcg tgacttttgt gctctctatt gtttgttccg
    13681 cattcggcat tttgtaaatg gaacgaggca ctgcttctgg cggtgcctct ttacttaagg
    13741 aatttcatcc tgtgcaaacc cttcagcaag ttgaaaacta tacggcgtta agtgaacgag
    13801 ccagcgaata tttattggct gtgatccgta gcaaaccgga tgccgtgatt tgcctggcga
    13861 ccggagccac gccattactg acgtatcatt atctggtaga aaaaatccac cagcagcagg
    13921 ttgatatcag ccagctcacc ttcgtgaagc tcgacgaatg ggtggatctg ccattaacga
    13981 tgcctggcac ctgcgaaact ttcctacaac agcatatcgt gcagccgctg gggctgcgtg
    14041 aagaccagct catcagcttt cgctccgaag agataaatga gacagagtgc gaacgggtaa
    14101 cgaacctgat tgcgcgcaaa ggcggtctgg atttatgcgt tctaggattg gggaaaaacg
    14161 gtcatcttgg gctgaacgaa ccgggagaaa gcctacaacc ggcctgccat atcagtcaac
    14221 ttgatgccag aacacagcaa catgagatgt taaaaaccac gggtcgcccc gtgactcgtg
    14281 ggatcacctt aggcctgaag gatattctca atgcccgcga ggttttgtta ttggtgactg
    14341 gcgaaggaaa gcaggatgcg acagaacgtt ttctcacggc taaagtctcc accgctatcc
    14401 cggcttcgtt tttatggctg cacagtaact ttatttgttt aattaatacc taaccagatg
    14461 aaaatttgct caaaattttt aaattaactt attcaacagt cacgcattat attaaataac
    14521 atttgactgg gttgaacata acgccaatag caaaaggagt tatgttcaac aatttaattt
    14581 tagatataca tataacttgt tctattaata aatcacctcg tattctgact cggcccctgg
    14641 cgcagcaaag tcaggcattt atactctttt taacacatag aaaattcata tcaatataat
    14701 aaaaacataa aatatttgca tataaatata atcttaaagt ttagtctatt taatgttcaa
    14761 tgaaatattt ctacctgtat aatctttaaa gatgttgaac atatattcac attaaatatg
    14821 attatgtact tgttacaagg ataaggttat aaatgaataa agttacaaaa acagctattg
    14881 tgggcttact tgcgcttttc gcaggtaatg ccgctgcaac cgatggtgaa atagtttttg
    14941 atggcgagat tttaaaatcc gcttgtgaaa tcaatgactc tgataagaaa attgaagttg
    15001 ctcttggtca ctataatgcc gaacagtttc gttctgttgg cgatcgtagc ccaaaaattc
    15061 cattcactat tcctttagtt aactgcccag tgactgggtg ggagcacgat aacggcaacg
    15121 ttgaagcgtc tttccgttta tggctggaaa cgcgggataa tggcaccgtc cctaacttcc
    15181 ctaatctggc aaaagtgggc tcttttgctg gcacagcggc aacaggtgtg ggtatccgta
    15241 ttgacgatgc ggaaagcgga aaccttatgc cactgaatgc tatggggaat gataacacgg
    15301 tttatcagat cccagcggac tccaatggta ttgtcaatgt tgacctcatc gcttactacg
    15361 tatcaaccgt tgaagcatca gaaatcaccc caggggaagc agacgctgtc gttaacgtaa
    15421 cgctggatta ccgttaatac gttacggcgt tatctgacct ttcagataac gcccttttcc
    15481 ttcctctttc tcgttgtatc aggttgaaaa ataactatgt caaaacgaac attcgcggtg
    15541 ataataacct tgttgtgtag cttctgtatc ggccaggcgc ttgcaggggg tatcgtttta
    15601 cagcgaacgc gagtgatcta tgatgccagc cgcaaagagg ctgcgttacc tgtcgcaaac
    15661 aaaggcgcag aaacgcctta tttactgcaa tcatgggtag ataatataga tggtacaagc
    15721 cgtgccccat ttattataac cccaccgcta tttcgtcttg aagctggcga tgactcatca
    15781 ctgcgaatta ttaaaacagc tgataacctg cctgaaaata aagagtcgct gttctacatt
    15841 aatgttcgtg ccattccagc aaagaaaaaa tcagatgatg ttaatgctaa cgagttgacg
    15901 ctggtattta aaacacggat caaaatgttt tatcgccccg cacacctgaa gggacgggta
    15961 aacgatgcgt ggacatcact ggaatttaaa cgtagtgacc attcactcaa tatatataac
    16021 ccaactgaat attacgtcgt atttgccgga ctggcagtcg ataaaaccga tctcacaagc
    16081 aaaattgaat atatcgcgcc cggagaacat aaacagttac cacttcctgc atctggcgga
    16141 aagaacgtga aatgggctgc aatcaatgat tatggcggca gttccgggac agaaactcgt
    16201 ccactgcaat aacaaatata aaaaacacag gtcatcaggg aatgccacaa cgacaccacc
    16261 agggacataa acgcacaccg aaacagttgg cgctcatcat caaacgctgt ttgccgatgg
    16321 tgctcactgg cagcggcatg ctttgcacta ccgctaacgc cgaagagtat tatttcgacc
    16381 ccattatgct ggaaactaca aaaagtggta tgcaaacaac tgatctgtca cgtttttcaa
    16441 aaaaatacgc acaactacca ggaacttatc aggttgatat ctggctgaat aaaaagaagg
    16501 tttcacagaa aaaaattaca tttaccgcca atgcagagca acttctgcag ccacagttta
    16561 cggtagaaca actacgtgag ctgggtatta aggtggatga aatcccggcg ctggctgaaa
    16621 aagatgacga tagcgtgatc aactcgcttg aacaaatcat tcccggtaca gctgctgaat
    16681 ttgatttcaa tcatcagcga cttaatttga gcattcccca aattgcactg taccgtgatg
    16741 caagaggtta cgtctcccct tctcgttggg acgatggtat accaacgctg tttaccaact
    16801 actcgtttac aggttctgat aaccgttacc gccagggcaa tcgtagccaa cgacagtacc
    16861 tgaatatgca aaatggtgct aattttggcc cctggcgatt acgtaactat tctacgtgga
    16921 cacgcaacga tcaggcgtca agctggaaca ctatcagtag ttatttacaa cgtgatatca
    16981 aggcgttgaa gtctcagttg cttctgggag aaagcgccac cagcggcagt attttttcca
    17041 gctacacctt tactggcgtg caactcgctt ccgacgataa tatgttgcca aacagccagc
    17101 gcggatttgc cccaacggta cgcggtatcg caaacagtag tgcaatcgtg actatcaggc
    17161 aaaatggtta tgtgatctat caaagcaacg tgccagcggg tgcctttgaa attaacgatc
    17221 tctacccctc ttccaacagc ggcgatttag aagtcacgat tgaagaaagt gacggtacgc
    17281 aacgtcgctt tatccagcct tattcttcat tacccatgat gcagcgacct gggcatctaa
    17341 aatatagcgc gaccgctgga cgctatcgcg ctgatgcaaa cagtgatagc aaggaacccg
    17401 aatttgctga agccacggca atatatggtt tgaataatac ttttacgctg tatggcggcc
    17461 tgctcggttc tgaagattat tatgcgctgg ggatcggtat cggcggcaca cttggcgcac
    17521 tgggcgcgtt gtcgatggat atcaacagag ctgacaccca attcgataac cagcactctt
    17581 ttcatggcta tcaatggcgt acgcagtaca tcaaagatat cccggaaacc aacaccaata
    17641 tcgctgtcag ctactatcgc tataccaacg atggctattt tagttttaat gaagccaata
    17701 cccgtaattg gaactataac agtcgccaaa aaagtgaaat tcaattcaac atcagccaga
    17761 caatatttga tggggtaagt ctgtatgcct ccggttcgca gcaagactat tggggcaata
    17821 acgagaaaaa caggaatatc tctgttgggg tttccggtca gcaatgggga attggttaca
    17881 gcctgaatta tcaatacagt cgctacacgg atcaaaataa tgaccgcgca ctctctttga
    17941 atctcagtat tccgttagaa cgctggttac cgcgtagccg ggtttcctat cagatgacca
    18001 gccagaaaga tcgcccaacc caacatgaaa tgcgtcttga tggctcactg ctggatgatg
    18061 gtcgcctgag ctatagcctg gaacaaagtc tggatgacga taacaaccat aacagtagcc
    18121 tgaacgccag ttaccgttca ccttatggca ccttcagtgc cggatacagc tacggtaatg
    18181 acagcagcca atacaattac ggcgttaccg gcggcgtggt tatccatcct catggcgtga
    18241 cgctctcgca atatctgggc aacgcttttg cgcttatcga tgctaatggg gcttctggcg
    18301 tgaggataca aaactatccg gggattgcta ctgatccctt tggctatgca gtggttcctt
    18361 atctcacgac ttaccaggaa aaccgtctct cggtagatac tacgcagctg cccgataacg
    18421 tcgatcttga gcaaacaaca cagtttgtgg tgcccaacag aggtgcaatg gtagcggcgc
    18481 gtttcaacgc caatatcggt tatcgcgtac ttgttacagt cagcgatcgc aacggtaaac
    18541 cgttgccctt tggcgctctt gccagcaacg atgagacggg gcaacaaagt atcgtcgatg
    18601 agggcggcat actatatctc tctgggatat cgagtaaatc acaaagctgg actgtacgct
    18661 ggggaaatca ggcagatcaa caatgtcagt ttgcttttag tacaccggat tcagaaccaa
    18721 caacctctgt attacaaggc acggcgcagt gccattaagg ataaaaaaat gaaaagagcg
    18781 cctcttataa caggactttt gttgatatcc acatcctgcg cttatgcctc ctcaggaggg
    18841 tgtggagctg acagcactag cggtgcgaca aattacagca gtgtggttga tgatgttacg
    18901 gtgaaccaga cagataacgt gacaggacgg gagtttacct ctgccacgct aagtagcact
    18961 aactggcaat acgcctgtac ctgctctgcg ggtaaggcag ttaaacttgt ctatatggtc
    19021 agccctgtac ttaccaccac tggacatcaa gcaggatatt acaaactcaa tgatagtctg
    19081 gatattaaaa ccacattaca ggcgaatgac attccaggac tcgtgactga ccagaccgtt
    19141 tctgttaaca cccgattcac acagataaaa agcaacaccg tatattctgc tgcaacccaa
    19201 acgggtgttt gccagggtga cacgtctcgt tatggacccg ttaatattgg tgcaaatacc
    19261 acctttaccc tgtatgtcac caagccattt ctcggctcga tgaccattcc gaaaacggat
    19321 attgccgtca ttaaaggcgc atgggtcgat ggaatgggaa gcccgtctac aggtgacttc
    19381 catgatttag tcaagttatc gattcaggga aatctcaccg ccccacagtc gtgcaaaatt
    19441 aatcagggcg atgttattaa ggttaatttt ggattcatca atggtcagaa gtttaccacc
    19501 cgcaatgcca tgccagacgg ttttactcca gtagactttg atatcactta tgactgtggt
    19561 gatacttcaa agattaaaaa ctcgttgcaa atgcgcatcg acggtacaac tggggtagta
    19621 gaccagtaca acctggtcgc cagacgaaga agttcagaca atgcgcccga tgtcggtatt
    19681 cgtattgaaa atctcggcgg cggagttgca aatattcctt ttcagaacgg tatccttccc
    19741 gttgatcctt ccgggcatgg caccatcaac atgcgcgcct ggccagttaa tctggtcggt
    19801 ggtgagctgg aaacaggaaa atttcagggc accgccacca ttaccgtcat cgtgcggtaa
    19861 aagaagacgt tgcgttttga ttaacgcaac gcttcggcac tcttacccct gctgctccag
    19921 cgcatactta tacagcgcat ttttcttcac gccgtgaatt tctgccgcca gcgccgccgc
    19981 ttttttcaac ggcagttctg cctgtagcag cgccagcgtg cgcagggcat cggcgggtaa
    20041 gtcttcttcc tgtgctttat gaccttcgat aatcagcacc atttcgcctt tgcgacggtt
    20101 ttcatcttct tttacccacg ccagcagctc gccaacgggc gcgccgtgaa tggtttccca
    20161 ggttttggtc agctcacgcg ccagaaccac gtagcgggat tcgcctaata ccgcaacgat
    20221 atcttccagg ctatctaaca gacggtgggt ggattcataa aaaatcagcg tgcgcggctc
    20281 cgcttcaatg gcttttagcg catcacggcg gccttttgat ttggcaggta aaaagccttc
    20341 gtaacagaaa cggtcagagg gtaaacccgc ggcgcttaac gcagtgatag cagcacacgg
    20401 cccgggtagc ggcaccacgc aaatccccgc ttcacggcag gtacgcacca gatggtagcc
    20461 aggatcgtta attagcggcg ttccggcatc ggaaaccagc gcaatgttct gccctttttg
    20521 cagcttcgcc agcagcgttt cggctttttg ttgttcgtta tggtcgtgca gcgcaaacaa
    20581 ccgggcatta atcccaaaat gttgcagcaa taaaccggtg tgacgagtat cctcggcggc
    20641 aatcagatca acggcctgta atacctctaa cgcacgctgg gtgatatccg ccagattgcc
    20701 gattggcgtc ggtacaatgt aaagctggcc ctgagaatta tccgccgatt ggtgttgttt
    20761 cattgtgtcg tccgtattgc cgatttaata ttgagcattg cgtaaaaaaa tatcactgga
    20821 tacattatgg taccctcaac attttctcgt ttgaaagccg cgcgttgtct gcctgttgtt
    20881 ctggcagccc tgattttcgc cggttgtggc acccatactc ccgatcagtc cactgcttat
    20941 atgcagggca cggcgcaggc tgattctgcc ttttatcttc agcagatgca gcaaagctct
    21001 gatgatacca ggatcaactg gcaattactc gccattcgtg cactggtgaa agaaggtaaa
    21061 accgggcagg cggttgagtt gtttaaccaa ctaccgcaag aactgaacga ttctcagcgt
    21121 cgcgagaaaa cactgctggc ggtagagatt aaactggcgc agaaagattt tgctggcgcg
    21181 caaaacttgc tggcgaaaat cacacctgcc gatttagaac aaaaccagca agcgcgttac
    21241 tggcaggcaa aaatcgatgc cagccagggg cgtccttcca ttgatttact gcgcgcgtta
    21301 attgctcagg aaccgctgct cggcgcgaaa gaaaaacagc agaatattga tgccacctgg
    21361 caggcgctct cctccatgac tcaggaacag gcgaatacgc tggtgatcaa cgccgacgaa
    21421 aatattctgc aaggctggct ggatctgcag cgcgtctggt ttgataaccg taacgatccc
    21481 gacatgatga aagccgggat cgccgactgg caaaaacgtt atccgaacaa tccgggcgcg
    21541 aaaatgctgc caacgcagtt ggttaacgta aaagcgttta aaccagcctc gaccaacaaa
    21601 atcgccctgc tgttgccgct gaatggccag gcagcggtat ttggtcgcac tattcagcaa
    21661 ggctttgaag cggcgaaaaa tatcggcact cagccagtgg cggctcaggt agctgccgca
    21721 cctgccgcag acgtagctga acaacctcag ccgcaaaccg tggatggcgt tgccagcccg
    21781 gcacaagcct cggttagcga tctgaccggt gaacagcctg cggcacagcc agcgcctgta
    21841 agcaccccgg cagcaaccac cacagcggta agcgcgccag cgaatccatc cgcagaactg
    21901 aaaatctacg atacctcatc acaaccactt agccagatct taagccaggt tcagcaggat
    21961 ggcgcgagta ttgtggttgg tccgttgctg aaaaataacg ttgaagagtt gcttaagagc
    22021 aacaccccgc tgaacgtgct ggcactgaac cagccggaga atatcgaaaa ccgcgtcaat
    22081 atttgttact tcgcgctttc accggaagac gaagcgcgcg atgcagcgcg tcatattcgt
    22141 gaccagggta aacaagcgcc gctggtgctg atcccacgca gttcattggg cgatcgcgta
    22201 gccaatgcgt ttgcgcagga gtggcagaaa ctgggcggcg gcaccgttct gcaacaaaaa
    22261 tttggttcca ccagcgaatt acgcgcgggt gttaacggcg gttctggtat cgctttaacg
    22321 ggtaccccga ttactcccag agcgacaacc gactccggca tgacgaccaa caatccaacg
    22381 ctgcaaacca cgccaaccga tgaccagttc accaataatg gcggtcgtgt cgatgcggtg
    22441 tacattgtgg caacgccggg tgaaatcgct tttatcaaac cgatgatcgc catgcgtaac
    22501 ggtagccaga gcggtgcaac gctgtacgcc agctcccgca gtgcgcaagg gaccgctggc
    22561 ccggatttcc gactggagat ggaaggcttg cagtacagcg aaatcccgat gctggcaggc
    22621 ggtaatctac cgttaatgca gcaggcactc agcgcggtga ataacgatta ttcactggct
    22681 cgcatgtatg cgatgggcgt cgatgcctgg tcgctggcaa atcatttctc acaaatgcgc
    22741 caggttcagg gttttgaaat caacggtaat accggaagcc tgacggcaaa cccggattgc
    22801 gtgattaaca ggaagttatc atggctacag taccaacaag gtcaggtagt ccccgccagt
    22861 taaccaccaa acagaccggc gatgcgtggg aagtacaagc gcgtcgctgg ctggaaggca
    22921 aaggactgcg atttgtcgcc gctaacgtga acgagcgtgg cggcgagatc gatctgataa
    22981 tgcgtgaagg ccggaccacc gtttttgtcg aggtgcgtta ccgccgctct gcgctttatg
    23041 gcggcgcggc agccagtgtg acccgcagca aacaacacaa attattacag actgcccgct
    23101 tgtggctcgc gcgtcataat gggagttttg atactgtgga ttgccggttc gatgtggtag
    23161 ccttcaccgg gaatgaggtt gagtggatta aggatgcctt taatgaccac tcataattaa
    23221 ggtttaagga ttagcgtgca agaaagaatt aaagcttgct tcactgaaag cattcaaact
    23281 caaattgcgg cggcagaggc gcttccggat gccatctccc gtgcagccat gacgctggtt
    23341 cagtctctgc tcaatggcaa caaaatcctc tgttgtggta atggaacttc cgctgccaat
    23401 gcacagcatt ttgctgccag catgatcaac cgtttcgaaa cggagcggcc cagcttacct
    23461 gccattgcac taaatactga taatgttgtc ttaacggcga ttgccaacga tcgcttacat
    23521 gatgaagtgt atgcaaaaca ggtgcgggcg ctgggtcatg cgggagatgt attgttagcc
    23581 atttccaccc gtggcaacag ccgcgatatt gtaaaagcgg tagaagctgc cgttacgcgt
    23641 gatatgacta ttgtggcatt gaccggctat gacggcggcg aacttgcagg tttgttaggg
    23701 ccacaagatg tggagatccg cattccttcg catcgtagtg ctcgcattca ggaaatgcat
    23761 atgctgacgg taaattgcct gtgcgatctg atcgataaca cgcttttccc tcaccaggat
    23821 gtttaaggag aatacatgaa ggcattatcg ccaatcgcag tccttatttc cgcgctgctg
    23881 ttgcaaggtt gtgttgccgc tgccgtagtg ggtaccgctg ctgtgggtac caaagccgca
    23941 actgacccac gcagtgtcgg cacccaggtg gacgatggta ccctggaagt gcgcgtgaac
    24001 agcgcattgt cgaaagacga acagattaag aaagaagccc gtattaatgt cacggcgtat
    24061 cagggcaaag tgctgctggt tgggcagtca ccaaacgctg aactttcggc tcgcgccaaa
    24121 cagattgcta tgggcgtaga cggtgccaac gaagtgtata acgagattcg tcagggccag
    24181 ccgattggtc tgggcgaagc gtctaacgat acgtggatca ccaccaaagt gcgttcgcag
    24241 ctcttaacca gcgacctggt gaaatcgtcc aacgtgaaag tgaccaccga aaacggtgaa
    24301 gtgttcctga tggggctggt gactgaacgt gaagcgaaag cggcggcaga tattgccagc
    24361 cgggtgagcg gcgtaaagcg ggtaaccaca gcgtttacgt ttattaaata gcagcccctt
    24421 gtaatgcctg atgcgacgct tgccgcgtct tatcaggcct tgtaggacag cgttgccagc
    24481 tcggatacgg cttcccgcgt taagacacac tatcccaaca actcttcctt aacatccatc
    24541 gccagctcaa acgaatgcag ccgcgcctgg tggtcgaaaa tctgcccgtt gaccataatc
    24601 tcatcggcgt cggtttcgcg caggatcgac tgcaagccat gacgcacttt cgctttatca
    24661 cctaccaacg acatactcag cgcctgctgc acaccatact gttcggacgg cgaccagaac
    24721 tgatccatat tttgaatcgg tggcggcagt tgcccggttt cgccacggcg caacttcaca
    24781 aaggcttgct gcattgaggt aaacagaaac tcagcgtcgc ggttgctatc ggcggcgata
    24841 atattgatgc acaccatcgc gtatggtttt tccagtcgtg ccgacggttt gaagttgctg
    24901 cgataaagat gcagcgcctg gaacaacata tccggcgcga agtgtgaggc aaacgcgaac
    24961 ggcagaccaa gctgcgccgc cagttgcgcg ctgtaaaggc tggagcctaa caaccacacg
    25021 gggattttct cgccatagcc tggtaccggg cgcacatgcg gattgggatc gcgggcgtca
    25081 aaccagtcca ccagctccgc gacatcgcgg gggaaattat caatatcgcc gctcatatgg
    25141 cgacgcagcg ccatcattgt ccgttggtca ctacctggcg cacgacccag ccccaaatcg
    25201 attcgccccg gatagagtgt attgagcgtg ccgaactgtt cggcaatgac caacggtgag
    25261 tggttaggca acatcacgcc gccagacccc agatgcagcg tggtggtatt cgccgccaga
    25321 tagccaatca ataccgacgt ggcagcactg gcaatgccag tcatattgtg gtgttctgcc
    25381 agccagtagc gatgatagcc gcgcttttca gccagacggg cgagatcgag agagtgggag
    25441 aatgcttctc gcgctgaaga accttcggga atgggggcca gatcgagtag cgaaaacgca
    25501 atggttttat cagtcataac agctcacttt gttaccgatt aatctttaat actgcattaa
    25561 agagtagtta aagttgttaa caaagtgagc tgtgctctaa ataattcgag ttgcaggaag
    25621 gcgacaagag agtgaatccc caggagctta cataagtaag tgactggggt gaacgaatgc
    25681 agatgcagca catgcaactt gaagtatgac gagcatattt acttaggctt gcagctccag
    25741 cccggccagc cgccgccagt agccgttaca gtcgctgttt gccgtcaacg gtagtggcgc
    25801 agcgccattt tcattagcgc ggaaggcgtc gagcatcgcg aaagtgtcag taccctgcgg
    25861 tgacaggcgg accacatcaa ccagcccctg catggatgcc agctcgttac cgaggttgta
    25921 aacgtagccg ctcatggtct gaatgccatt gagtacaaac acttgttggt tttcctgcga
    25981 cagcacgttg cgcccgttcg gatacttaat gcagcaggtt tcacactcat ctttcgggcg
    26041 gtcttccgaa cgcgcggtaa agcagcgggc ggagtaggcc agcggtagat gaccgtagct
    26101 caggacttcc acttcaaact ggttgctaat gcccagctca tcgcactgat taagcagatt
    26161 caccagccag tcgcgggaaa gctccaccgg catacaccag cgcatcatgc cctgtttgag
    26221 caatattttc agcgtcaccg cgttgtagca gttcagcgcg tgcccggcga cgaacggcag
    26281 tttgcgttca gcgcacatat tcaccacgcc gagatcgctg gcttcaatca gaaactcacc
    26341 gttctcaaca tagcgtttca gttcgcccag ttcagaggat gcctgcacca gcgccagcgt
    26401 ggagagcaca atctgcttgc cacttccggc gagcgatttt gccatctcca gccagtcgcc
    26461 aactttggtt gcccggcgct tgctgcatac cgcttcacca agataaatca cgtcggcgct
    26521 gctggtggcg gcctgctgat aaaaatcttc cagcgtctct tttggccagt accacagcac
    26581 tggccctaag gaatatttca ttgctcttct cactgccatt tacggtgata cgcgccaagg
    26641 gtggtctgcg tgccttcgga catcgacccg agcgtctcca tccacgcgct ttgcggtatg
    26701 aagttttgcg gatcggcctt acaacggtcg atagcctgac gccagacttt cgccacctgg
    26761 ctgacatacg ccgggctacg ctggcggcct tcaattttca ccgaagcaat attcgccgcc
    26821 attaactccg gcagcagttc cagggtattg agactggttg gttcttccag cgcgtgatag
    26881 cgctcgccgt ccaccagata acgcccttta catagcgtcg gataacctgc gttttcgccg
    26941 tcctgataac ggtcgatcag cacttcgttc aggcgggatt ccagcccctg cggcgtttgt
    27001 tgccagcgca cgaaacgggc cggagaacac gcgcctacgg tgttgggcga ctcacccgtc
    27061 agatacgacg acagatagca acgaccttcc gacataatgc acaggctgcc gaaagcaaag
    27121 acttccagtg gtacaggtgt gacccgtgcc agttgtttca cctgatgaat cgacaacacg
    27181 cgcggcagca ccacgcgagc aacgtcaaaa tggcgatgat aaaagttaat cgcctcttca
    27241 ttggtcgccg aagcctgcac tgacacatga cgctcaatat gcgggtaacg ctcggcggcg
    27301 tactctagca tggcgaggtc ggcgaggatc agcgcgtcgg cacccagctg cgccgccata
    27361 tccacggcac gctgccaacg ggcgtaaccg tccggatgcg caaaagtgtt aatcgcgatg
    27421 tgaagtttgc ggcgatgttg atggacaaaa ctcaccgctt cctgcaattt tttctcggta
    27481 aagttaaggc cggcgaagtg acgggcattg gtatcatctt ttagcccgat ataaacagca
    27541 tctgcgccgt tttcgatggc cgccttaagc gccgggagat ttccggcagg gcagagcagc
    27601 tccataattt ttcctgaagg ttgcgctatc aaaacgcaaa aatgttaacg aactgggatt
    27661 ttagttaacc ttgctgcgac aatttttgat ttaaggcagt taaagttgta ttggtggtag
    27721 cagcaatttc atatggaatt gttgatttat accgctatgt tatttttctg atatggcaaa
    27781 ataggatgat tgttgaaaca gggagtaaaa ctcgtgttgg ataaactgcg ttcccgtatt
    27841 gtgcatttgg ggccatctct gttgagtgta ccggtaaaac tgacgccatt tgcgctaaaa
    27901 cgccaggttc ttgagcaggt cttaagctgg caattccgtc aggcgctgga tgatggcgag
    27961 ctggagtttc ttgaaggccg ctggttaagt attcatgtgc gtgatattga cctgcaatgg
    28021 tttacctcgg tggtgaatgg caaactggtg gtcagccaga acgcgcaagc tgatgtgagt
    28081 tttagtgccg acgccagcga tctgctgatg attgcggcgc gtaaacaaga tccggatacg
    28141 ctcttcttcc agcgtcggct ggtgattgaa ggcgatacgg agctggggct gtatgtgaaa
    28201 aacctgatgg acgccattga actggagcaa atgccgaaag ccttacgcat gatgctgctg
    28261 caactggcgg attttgttga ggcgggaatg aaaaccgcgc ctgaaaccaa acagacatcg
    28321 gtaggtgaac catgctaatt cgagtagaaa ttcccattga tgcgccgggt attgatgccc
    28381 tgctacgtcg ctcattcgaa agtgatgcgg aagcgaagct ggttcacgat ctgcgtgaag
    28441 atggctttct gacgctgggg ctggtggcga cagatgacga aggtcaggtc attggctatg
    28501 tggcatttag tccggttgat gtgcagggcg aagacctgca atgggtcggc atggcaccgt
    28561 tggcggttga tgaaaaatac cgtggacagg ggctggcacg ccaactggtc tatgaaggac
    28621 tcgattcgct taatgagttc ggctatgccg cagtggtgac gctgggcgat ccggcgctgt
    28681 acagccgttt tggctttgaa ctggcggcac atcacgacct gcgttgccgc tggccaggca
    28741 ctgaaagcgc cttccaggta catcgtttag cggatgacgc gttgaatggc gtaactggtc
    28801 tggttgagta tcacgagcat ttcaatcgct tttaatctcc ggggtttgca gactgcttaa
    28861 cagctctgca aaccctgcgc tttccgctac cagacgctct ttttgccgtt tggtcagttg
    28921 cttaacgcga tattccgccc gtaacgccag cgaacgatcg cccactggcg cagaaaacgc
    28981 cagcgtcagt tctcctttcc cgcgcagtgc tttcgcccct ttgccgcttt ggtgctgctg
    29041 atagcggcgt tcgacatccg tggtgatccc ggtataaagc ttattgtcgg cggtacggat
    29101 caagtaaaga aaccagggtg tcatcgtctg cgtcgcatgt taaggtcagg cccacagtat
    29161 aaaagagaac agcaatggaa acactcatca ccatcagccg ttggttggca aaacaacatg
    29221 ttgtcacctg gtgtgtgcag caggaagggg aactctggtg cgctaatgct ttttatctgt
    29281 ttgatgcgca gaaagtcgcc ttctacattt tgacggaaga aaaaacgcgc cacgcgcaga
    29341 tgagtgggcc gcaggctgcg gttgccggaa cggtaaacgg tcagccgaaa acggtagcgt
    29401 taattcgcgg cgtgcagttt aaaggtgaga tccgcaggct ggaaggtgag gaaagcgacc
    29461 tcgcgcgcaa ggcgtacaat cgtcgctttc cggttgccag aatgctgtcg gccccggtgt
    29521 gggaaatccg gctcgatgaa atcaaattca ccgacaacac gctgggcttt ggtaaaaaaa
    29581 tgatttggct acgcggctca ggcaccgagc aggcgtaacg cctcgcggtt aaacgctggc
    29641 agatcgtccg gtgtccggct ggtaaccagc tgatctttat cgaccacgac ttcctgatcg
    29701 taaaattccg cgcccgcgtt tttaacatca ataatgatcg gtttaaccgc ggtcagtttg
    29761 cgcccccgaa tcacatcggc gctgatcagc agctgcggac cgtgacagat ggcgaaaaca
    29821 ggtttgccgc tattcacaaa atcacgggta aaggtgacaa aacggttgtc gccacgcaga
    29881 taatccggtg aatggccgcc cggtagcagc agggcatcaa actccgctgg cgtcacttca
    29941 tcgatggatt tatcgatggt cacgctggct tctccttttt tgcctttcac cgttttaccc
    30001 gcttgttttt caatggtaat cacttcgtgt ccggctttac ggaactcgtc tgcgggtgaa
    30061 gtaaattctg aatcttcaaa ttcatcagtg attaaaacgg caattttctt actcatgctt
    30121 cctccgcgtt gttgcattgg atgaggcgaa tttcccacaa tcggggataa ttacctcatg
    30181 gtctattaag cctggtgcat gagccgtgga gcgcaaggca gacaattctg taaaggagcg
    30241 aagatgagtc aggtactgat tacaggcgca acggggctgg tgggcggtca cctgctgcgg
    30301 atgttgatta acgaaccgaa agttaacgcc atagccgcgc cgacgcgacg tccgttgggc
    30361 gatatgcccg gggtgtttaa tccccatgat ccgcaactga ctgacgcgct ggcgcaggtc
    30421 accgatccca tcgacattgt gttttgttgt ctcggcacca cgcggcgaga agcggggagc
    30481 aaagaggcgt ttattcatgc cgattacacg ctggtagtgg ataccgcatt aaccgggcgg
    30541 cgactgggcg cgcagcatat gctggtggtc agtgctatgg gtgccaatgc ccactcgccg
    30601 tttttctata accgcgtcaa aggggagatg gaagaagcat taatcgccca gaactggccg
    30661 aaattgacca ttgctcgccc gtcgatgtta ctgggcgatc gtagcaaaca gcggatgaac
    30721 gaaacgcttt ttgcgccgct gttccgttta ttacccggta actggaaatc cattgatgcg
    30781 agagacgtgg cgcgggtcat gctggcagag gcgatgcggc ccgggcatga aggtgtgacg
    30841 attttaagct cttcagaatt acgcaaaaga gctgaataat tattcccaag aattgatagt
    30901 gagcgtaata ttcacgcgct caattatcat aacttattcc tggggaatgc tatgactggt
    30961 cagtcttcat ctcaggcggc aacgcccatt cagtggtgga aacccgcgct tttctttctc
    31021 gttgtcattg ccggtctctg gtatgtgaaa tggcaacctt actacggcaa agcgtttact
    31081 gctgccgaaa cccacagtat cggtaaatct atccttgcgc aggcggatgc taatccgtgg
    31141 caggcggcgt tggattacgc gatgatctat ttcctcgcgg tatggaaagc ggcagtgctg
    31201 ggggtgatcc tcggttcgtt gattcaggtg ctgatcccgc gtgactggtt gttgcgtacg
    31261 cttgggcaat cgcgctttcg cggcacgctg ctgggaacgc tgttttcgct gccgggcatg
    31321 atgtgtacct gctgtgcggc tccggtcgcg gcgggaatgc gtcgccaaca ggtgtcgatg
    31381 ggcggtgcgc tggcattctg gatgggcaat ccggtgttaa acccggcgac gctggtgttt
    31441 atggggtttg tcctcggctg gggttttgcg gcgattcgtc tggtggccgg gctggtgatg
    31501 gtgttgctga ttgcgacgct ggtgcaaaaa tgggtgcgtg aaacaccgca aacgcaggca
    31561 ccggtcgaaa ttgacatacc ggaagcacag ggcggttttt ttagccgctg gggcagggcg
    31621 ctatggacgc ttttctggag tacgatcccg gtttacatcc ttgcagtact ggtgttgggt
    31681 gccgctcgcg tctggttatt cccccatgcc gatggtgctg tcgataacag cctgatgtgg
    31741 gtgatggcga tggcggtagc aggatgcttg tttgtcattc ccacggcagc agaaattccg
    31801 attgtacaaa cgatgatgct ggcaggtatg ggaactgctc cggcgctggc attgttgatg
    31861 acgctcccgg cggtgagttt gccgtcactg attatgctgc gcaaagcgtt cccagcgaaa
    31921 gccttatggc tgacaggggc gatggtggca gtgtctggtg tgattgtcgg cgggctggcg
    31981 ctgttgttct gatttgaggg aaaacatgtc ggatgcggcg taaacgcctt atccgaccta
    32041 cggtttgcat cgttgtagat actgttatgg cctgcaagtt ttttcctggt ccggtttccc
    32101 ggattgcagg catgccagac acagcgccgt caggcgctgt ttttttcatc ccttccgcga
    32161 tttttacgcc gctaccggat tatgccgtga cgcatcgaac ggcacgcctg acttcagcac
    32221 tccatacgcc acctgtgcca gcttgcgcat catcgcgccg agaatcacct
//
LOCUS       DAZGVQ010000042        31879 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000042 DAZGVQ010000000
VERSION     DAZGVQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.042"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            39..482
                     /gene="cmtB"
                     /locus_tag="ACHLAG_001891"
     CDS             39..482
                     /gene="cmtB"
                     /locus_tag="ACHLAG_001891"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFP0655135.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIAFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            510..1898
                     /gene="cmtA"
                     /locus_tag="ACHLAG_001892"
     CDS             510..1898
                     /gene="cmtA"
                     /locus_tag="ACHLAG_001892"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFP0655136.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            1913..3190
                     /gene="yggP"
                     /locus_tag="ACHLAG_001893"
     CDS             1913..3190
                     /gene="yggP"
                     /locus_tag="ACHLAG_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP0655137.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            3187..4152
                     /gene="glpX"
                     /locus_tag="ACHLAG_001894"
     CDS             3187..4152
                     /gene="glpX"
                     /locus_tag="ACHLAG_001894"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP0655138.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MALAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            4174..4683
                     /gene="fumE"
                     /locus_tag="ACHLAG_001895"
     CDS             4174..4683
                     /gene="fumE"
                     /locus_tag="ACHLAG_001895"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFP0655139.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            4680..5393
                     /locus_tag="ACHLAG_001896"
     CDS             4680..5393
                     /locus_tag="ACHLAG_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFP0655140.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(5365..6042)
                     /locus_tag="ACHLAG_001897"
     CDS             complement(5365..6042)
                     /locus_tag="ACHLAG_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0655141.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCGQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(6036..6713)
                     /locus_tag="ACHLAG_001898"
     CDS             complement(6036..6713)
                     /locus_tag="ACHLAG_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0655142.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(6701..7408)
                     /locus_tag="ACHLAG_001899"
     CDS             complement(6701..7408)
                     /locus_tag="ACHLAG_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFP0655143.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPLGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNNLAVRGAALDMRAFRIQNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(7409..7987)
                     /locus_tag="ACHLAG_001900"
     CDS             complement(7409..7987)
                     /locus_tag="ACHLAG_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP0655144.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTELFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(8010..8441)
                     /locus_tag="ACHLAG_001901"
     CDS             complement(8010..8441)
                     /locus_tag="ACHLAG_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFP0655145.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            8813..9832
                     /gene="epd"
                     /locus_tag="ACHLAG_001902"
     CDS             8813..9832
                     /gene="epd"
                     /locus_tag="ACHLAG_001902"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFP0655146.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            9882..11045
                     /gene="pgk"
                     /locus_tag="ACHLAG_001903"
     CDS             9882..11045
                     /gene="pgk"
                     /locus_tag="ACHLAG_001903"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFP0655147.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            11260..12339
                     /gene="fbaA"
                     /locus_tag="ACHLAG_001904"
     CDS             11260..12339
                     /gene="fbaA"
                     /locus_tag="ACHLAG_001904"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP0655148.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(12403..12501)
                     /locus_tag="ACHLAG_001905"
                     /pseudo
     CDS             complement(12403..12501)
                     /locus_tag="ACHLAG_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12697..13557
                     /gene="mscS"
                     /locus_tag="ACHLAG_001906"
     CDS             12697..13557
                     /gene="mscS"
                     /locus_tag="ACHLAG_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFP0655149.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            13696..14331
                     /gene="argO"
                     /locus_tag="ACHLAG_001907"
     CDS             13696..14331
                     /gene="argO"
                     /locus_tag="ACHLAG_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFP0655150.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKAQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            14424..15164
                     /gene="yggE"
                     /locus_tag="ACHLAG_001908"
     CDS             14424..15164
                     /gene="yggE"
                     /locus_tag="ACHLAG_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFP0655151.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            15331..16227
                     /gene="ygfI"
                     /locus_tag="ACHLAG_001909"
     CDS             15331..16227
                     /gene="ygfI"
                     /locus_tag="ACHLAG_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0655152.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTSH"
     gene            complement(16224..17702)
                     /gene="scpC"
                     /locus_tag="ACHLAG_001910"
     CDS             complement(16224..17702)
                     /gene="scpC"
                     /locus_tag="ACHLAG_001910"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFP0655153.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(17726..18511)
                     /gene="scpB"
                     /locus_tag="ACHLAG_001911"
     CDS             complement(17726..18511)
                     /gene="scpB"
                     /locus_tag="ACHLAG_001911"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFP0655154.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(18522..19517)
                     /gene="meaB"
                     /locus_tag="ACHLAG_001912"
     CDS             complement(18522..19517)
                     /gene="meaB"
                     /locus_tag="ACHLAG_001912"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFP0655155.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLHHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(19510..21654)
                     /gene="scpA"
                     /locus_tag="ACHLAG_001913"
                     /pseudo
     CDS             complement(19510..21654)
                     /gene="scpA"
                     /locus_tag="ACHLAG_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(21858..22751)
                     /gene="argP"
                     /locus_tag="ACHLAG_001914"
     CDS             complement(21858..22751)
                     /gene="argP"
                     /locus_tag="ACHLAG_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFP0655156.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            22893..23078
                     /locus_tag="ACHLAG_001915"
     CDS             22893..23078
                     /locus_tag="ACHLAG_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655157.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            23134..23793
                     /gene="rpiA"
                     /locus_tag="ACHLAG_001916"
     CDS             23134..23793
                     /gene="rpiA"
                     /locus_tag="ACHLAG_001916"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFP0655158.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            24049..25281
                     /gene="serA"
                     /locus_tag="ACHLAG_001917"
     CDS             24049..25281
                     /gene="serA"
                     /locus_tag="ACHLAG_001917"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP0655159.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            25510..25569
                     /gene="ibsC"
                     /locus_tag="ACHLAG_001918"
     CDS             25510..25569
                     /gene="ibsC"
                     /locus_tag="ACHLAG_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFP0655160.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            25902..25961
                     /locus_tag="ACHLAG_001919"
     CDS             25902..25961
                     /locus_tag="ACHLAG_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0655161.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26062..26664)
                     /gene="fau"
                     /locus_tag="ACHLAG_001920"
     CDS             complement(26062..26664)
                     /gene="fau"
                     /locus_tag="ACHLAG_001920"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFP0655162.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(26685..26868)
                     /gene="ssrS"
                     /locus_tag="ACHLAG_001921"
     ncRNA           complement(26685..26868)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHLAG_001921"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(26910..27239)
                     /gene="zapA"
                     /locus_tag="ACHLAG_001922"
     CDS             complement(26910..27239)
                     /gene="zapA"
                     /locus_tag="ACHLAG_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP0655163.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            27407..27985
                     /gene="ygfB"
                     /locus_tag="ACHLAG_001923"
     CDS             27407..27985
                     /gene="ygfB"
                     /locus_tag="ACHLAG_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFP0655164.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            28011..29336
                     /gene="pepP"
                     /locus_tag="ACHLAG_001924"
     CDS             28011..29336
                     /gene="pepP"
                     /locus_tag="ACHLAG_001924"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFP0655165.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPIVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSMQEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            29333..30511
                     /gene="ubiH"
                     /locus_tag="ACHLAG_001925"
                     /gene_synonym="visB"
     CDS             29333..30511
                     /gene="ubiH"
                     /locus_tag="ACHLAG_001925"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFP0655166.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            30534..31736
                     /gene="ubiI"
                     /locus_tag="ACHLAG_001926"
                     /gene_synonym="visC"
     CDS             30534..31736
                     /gene="ubiI"
                     /locus_tag="ACHLAG_001926"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFP0655167.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(31810..>31879)
                     /locus_tag="ACHLAG_001927"
     CDS             complement(31810..>31879)
                     /locus_tag="ACHLAG_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655168.1"
                     /translation="KSKNNDIRKYNAKTEVKLVYFK"
BASE COUNT         7628 a         7903 c         8587 g         7761 t
ORIGIN      
        1 cgccggacgg taccctaaaa ataaaataag gataatttat gcggcttagt gattattttc
       61 cagaatcatc aatctcagtt atacattcag caaaagattg gcaggaagct atcgctttct
      121 cgatggtatc attgctggat aaaaactata tcagcgagaa ttacattcag gcaattaaag
      181 attccaccat taacaatggc ccttattata ttctcgcacc aggcgtggca atgcctcatg
      241 cgcgaccgga atgtggggcg cttaaaaccg ggatgtcttt gacattactt gaacaaggtg
      301 tttattttcc ggggaatgac gaaccaatta aattactcat cggactctct gctgccgatg
      361 ccgattcgca cattggcgct attcaggcgt taagtgaatt actgtgcgaa gaagaaatac
      421 tcgaacaact cttaacagca tcatcagaaa aacaattagc ggacattatc agccgcggat
      481 aattactctc tcctctacag gataatatta tggaaaacaa gtctgctcgt gcaaaggtcc
      541 aggcttttgg gggctttttg actgcaatgg tcatccccaa tattggtgct tttattgcct
      601 ggggttttat tactgcgtta tttattccca ccggttggct gcctaacgaa catttcgcca
      661 aaattgtcgg cccgatgatt acctatttat tgcccgtgat gattggttct acaggtggtc
      721 atctggtcgg cggtaaacgc ggggcggtca tgggcggaat aggtactatt ggtgtgatcg
      781 ttggcgcaga gatcccgatg ttccttggct caatgatcat ggggccgctc ggtgggttgg
      841 tcataaaata tgtcgataag gcactggaaa aacgcatacc tgccggtttt gagatggtta
      901 tcaataactt ctcattaggt atcgcgggga tgctcctttg tctgctgggt tttgaagtga
      961 tcggcccggc ggtgttaatt gccaatactt tcgtcaaaga gtgtatcgag gcgctggtac
     1021 atgcgggtta tctgcctctg ctgtcagtca tcaatgaacc ggcgaaagtg cttttcctta
     1081 ataatgcgat cgatcagggc gtctattatc cgctgggaat gcaacaggct tcggttaacg
     1141 gtaaatccat cttctttatg gtggcctcta acccaggtcc gggcctgggg ctgctgctgg
     1201 cgtttacctt gtttggtaaa gggatgagta aacgttctgc gcccggggcg atgattattc
     1261 acttcctcgg tgggatccac gaactgtatt tcccatatgt gctgatgaag ccgctgacta
     1321 ttattgccat gattgcgggc ggtatgtctg gtacctggat gtttaactta ctggacggtg
     1381 gtctggtggc tggcccaagt ccggggtcta tctttgctta cctggcactg acgccgaaag
     1441 gctcgttcct ggcgacaatt gccggtgtta cggtaggtac cctggtgtcc tttgctatta
     1501 cttcgctgat actgaagatg gaaaaaacgg tggaaacgga gagcgaagat gagtttgctc
     1561 agtcagccaa tgcggttaag gcgatgaaac aagagggtgc attctcgtta agcagggtta
     1621 agcgtatcgc ctttgtttgc gatgcgggga tgggctccag tgcgatgggc gcgaccacct
     1681 tccgtaaacg cctggaaaaa gcggggctgg caatagaagt aaaacattac gcgatagaaa
     1741 acgtgcctgc ggatgccgat atcgtcgtta ctcatgccag tctggaaggg cgcgtgaaac
     1801 gtgttacgga taaaccgctg atattgatta ataactatat tggcgatcca aaactcgaca
     1861 ctttatttaa tcaattaacc gccgaacata aacactgatt acagaggtaa aaatgaaaac
     1921 caaagttgct gctatttatg gcaagcggga tgtccgtctg cgcgaatttg aactgccaga
     1981 aattaccgat aatgaactgt tagtgagtgt aatttctgac agcgtctgtt tatcgacctg
     2041 gaaagcggcg ttactcggta gtgaacataa acgcgtaccc gacgatttag aaaatcatcc
     2101 ggtcattacc gggcatgaat gtgccggggt tattgtcgaa gtgggtaaaa atctcactgg
     2161 caaatataaa aaaggccagc gttttgtatt gcaaccggcg atggggttac caagcggata
     2221 ttcagcaggc tacagctacg aatattttgg cggtaacgcc acctatatga ttattcccga
     2281 aatcgccatt aatttgggct gcgtattacc gtatcacggc tcttattttg ctgcggcgtc
     2341 gctggcagag cctatgtgct gcattattgg tgcttatcat gccaattatc acaccacgca
     2401 atatgtttat gagcatcgca tgggcgtcaa acctggcggc aatattgcac tgctggcgtg
     2461 tgcgggtccg atgggcattg gcgctatcga ttacgccatt aacggtggca tacaaccgtc
     2521 acgggtggtg gtggtcgata tcgacgacaa acgcctggcg caggtgcaga agctgctgcc
     2581 ggtggatctg gcggccagta aaggcattga gctggtgtat gtgaatacca aagggatgag
     2641 cgatccggtc cagacgctgc gggcgctgac aggagatgtc gggttcgatg acatttttgt
     2701 ttatgcggcg gtgcctgctg tcgttgagat ggctgacgaa ttactggcgg aagatggctg
     2761 tctgaacttc tttgccgggc cgacggataa aaacttcaaa gtgccgttta atttctacaa
     2821 cgtccattac aacagcacgc acgtagtcgg tacatccggc ggttcaacgg acgacatgaa
     2881 agaggcgatt gcgttaagcg ccacggggca gttgcagccg tcctttatgg tcacgcatat
     2941 cggtgggctg gatgcggtgc cagataccgt gctcaatctg ccggatatcc ctggcggtaa
     3001 aaaactcatt tataacggcg tgaccatgcc gctcactgcc attgccgatt ttgccgaaaa
     3061 aggcaaaacc gatccgctgt ttaaagagtt ggcgcggctg gttgaggaaa cgcacggcat
     3121 ctggaatgaa caggccgaga aatatctgct ggcacaattt ggcgttgata tcggggaggc
     3181 cgcgcaatga tgtccctggc gtggccatta tttcgcgtta cggaacaggc agcgttggct
     3241 gcctggccgc aaaccggatg tggcgacaaa aataaaattg atggcctggc ggtcaccgcg
     3301 atgcgccagg cattaaacga cgtcgctttt cgtggacgag tggttatcgg cgaaggagaa
     3361 attgaccatg cgccgatgct gtggattggc gaagaggtgg gcaaaggcga tgggccagaa
     3421 gtcgatatcg cggttgaccc gattgaaggc acgcggatgg tagcgatggg gcagagcaat
     3481 gcgctggcgg tcatggcttt agcccctcga gatagcctgt tacatgcgcc cgatatgtat
     3541 atgaaaaagc tggttgttaa tcgactggct gccggggcga ttgatctgtc tttgcctctg
     3601 gcggacaact tgcgtaacgt ggcgcgggcg ttaggtaaac cgctggacaa gctgcgcatg
     3661 gttacgctgg ataaaccgcg cctgagtgcc gcgattgaag aagcaacaca gctgggcgtg
     3721 aaggttttcg ctctgccgga tggcgatgtc gccgccagcg tgctgacctg ctggcaggat
     3781 aatccttacg acgtgatgta caccatcggc ggtgcgccag aaggcgtgat ttctgcctgt
     3841 gccgttaaag cattaggtgg ggatatgcag gccgagctga ttgacttttg tcaggctaaa
     3901 ggggattaca cggaaaatcg gcagattgca gagcaggagc gcaagcgttg taaggcaatg
     3961 ggcgttgacg ttaaccgtgt gtactcgctc gatgaactgg tgagggggaa cgatatcctc
     4021 tttagcgcta cgggcgtgac gggcggcgag ttggtgaacg gtatccaaca gacggcgaat
     4081 ggggtgcgga cgcagacatt actgatcggc ggcgcggacc aaacgtgtaa tataatagac
     4141 tccctgcatt gatggtgatc tatcggaaga ccaatggcga cgctgacaga agatgatgtg
     4201 cttgagcaac tggatgcaca ggacaattta ttttcattta tgaaaactgc gcattctatt
     4261 ttgctccagg ggatacgcca gtttctgccg tcgctgtttg tcgataacga tgaagagatc
     4321 gtcgaatatg cagtgaagcc gttactcgcc caaagcggcc cgcttgacga tattgatgtt
     4381 gcgctgcgtt tgatttatgc gctggggaaa atggataaat ggctgtatgc cgatatcacg
     4441 cacttttccc agtactggca ttacctgaac gaacaggatg aaacgcccgg atttgccgat
     4501 gacatgacct gggattttat cagcaacgtc aatagcataa cctgcaatgc gacgctctat
     4561 gacgcgttaa aagcgatgaa gttcgccgat ttcgccgtct ggtcagaggc gcgttttagc
     4621 ggaatggtca aaacggcgct gacgctggca gtaacgacca ccttaaagga attaacgccg
     4681 tgaaaattga attaacggtg aatgggctga acgtgcaagc gcagtaccat gatgaagaga
     4741 tagaacgcgt gcataaaccg ttgctgcgta tgctggcggc cttacagacg gtaagtccgc
     4801 agcgacgtac ggtggttttt ctttgcgctc cgccggggac gggtaaatct accttaacta
     4861 ccttctggga atatctcgcg caacaagacc ctgaattacc cgctatccag acgctcccga
     4921 tggatggttt tcaccattac aatagctggc tggatgcgca tcaattgcgc cccttcaaag
     4981 gcgcaccaga gacatttgac gttgcgaaac tggcggaaaa tctgtgccag gtagtggaag
     5041 gggattgtac gtggccgcag tatgatcgac aaaagcatga tcctgttgaa gatgcgttgc
     5101 acgttaccgc accactcgtc atcgtcgaag gaaactggtt gttgctggac gacgagaagt
     5161 ggtgccagct tgcgcaattt tgtgatttct cgatctttat caacgcgcca gctactgcgt
     5221 tgcgggaacg actggtaggg cgcaagctgg cgggaggggt atcgctggca gacgcagaag
     5281 ctttttatga ccgtaccgac gggccgaatg ttcgccgggg gctggaagag agtctgcctg
     5341 caaatttgac cttaatgatg accgctacgg gtgaatatcg tctggtggat taacgttcct
     5401 gattagacca tcttccagtc gcaccacacg agagaaatga cgccgggtaa atgcggcgtc
     5461 atgactgatt gctacgacgc tcgtgcctcg ctggccgcac ttctccagcc agctttcaaa
     5521 cacgctcaac caattttcgt caaaatcccg actcggctcg tccagcagca atagtggcgg
     5581 agagagtgct tccaggcagg cgacggcgac catccgtcgc tgcgcactat gcagatccaa
     5641 cgggtgcgag ttggcagtgt ctgctaactg acaacattgc agggcagcat gggtacgttg
     5701 agtgatttca tccgccgggc atttttgcag tttcaaacca aatgcgatct cgtcagcgac
     5761 ggtgctgtga aaaagttggt tttccgcttc ctgaaacagt acgccaacct ttgccgcgcg
     5821 ctggcggttt ttcaggtttt tcatcgcctg ttgttgcagc ataaccgtac cggctgtggg
     5881 cgtaagaaga cctgccatta ctcgtaacag cgtcgatttt cccgcgccat tgtcgccggt
     5941 cagcgccaac cattcacctt gtttgagttg cagagaaatg tcgcaaagac aatccgttgc
     6001 cgcgccaggc cagcgataag atatcttatt taacgttaac atagtggctg gagcgctccg
     6061 ttttgcaatt gccacgcttg ctggcagtag ttcaggaaag gtgtgcgatg acgttcaaaa
     6121 agaataatca gcgaaccacg ttcaaacgcc cagtgttgta agcgctgcaa cagcatttcg
     6181 ctggcctgcg gagtcaggcg gctaaaggct tcatcaagaa ttaagagctt tggctgcatc
     6241 gcgatggcgc aggcgatgac cacgcgctgt gtctctccac cggagagtgt tgccgggtga
     6301 cgatggcgca acggctggca ctcggtcaga gtcagggcgg catcaatccg cgccataatc
     6361 tctgcttcag caagacacag attttctggc ccaaaggcaa cttcttcttc cacgctaaag
     6421 gtacagccgg agagctgcaa atagggcgat tgctggacga gctggatggt ggcggattgt
     6481 tcattaagcg ggagatgccc aatgggcgta ccgaggagcg tccccgtgcc tgttatctct
     6541 cctggcagaa agtccggata ccagcccgcc atcagctgtg ccagcgtact tttgccactg
     6601 ccgttgtcac caaagatggc gaccatcccc ggtttgacat agtgaaagtc ataacagaac
     6661 ggaggatgcg ttgagtgtgt cgggcaataa cggaactgtt ctaacgtaac catatccacg
     6721 ctccgaattc tgtcagcatc agcaagatca tggtgtaacg cgctacgcgt tgtagggtgc
     6781 tatcggctgg tgcccacaac gttgttcgtc ggttttggat ccgaaatgcc cgcatatcca
     6841 gcgccgcacc acggacagca agattgttta gcgcattgtg tgttagcgga ataattaacg
     6901 cgggcatcgc acgtaaacgc tgataccagc cctcatccag cggcacgccg cgggcacgct
     6961 gcgcttcatg aataatcgct aattgccgtt ttaactgctc aacgaccaat aacggcccgg
     7021 caaacagata agcgacgcct agcggcaggc gagaagcaaa cagagcgcgg ataaaacatt
     7081 gcacggggac gtactgcatc cacaactgtg aggttgaaac aatcgccaga atccgcaacc
     7141 agagcgtaat agcatgcgac caccgttctg gcgagcgtgg cgtgccgctt agccattctg
     7201 gtagccagcc accgtgtacc agccacagcc cggctcccag cgagaacatc agccagacga
     7261 catattttgc ccgccgccgc gtggccttta gcgcaataag gcagaaaaag gtcgccgcgc
     7321 tatagatcgg caaaatggtc tgcgcgggca ggataagcgt ggtgcaggcc gccaatgccc
     7381 acagtgttaa tgaggtaaat ggatgcattt agcgcacggc agccattgcc ggaaaatggc
     7441 gcgtggtgcg aatcggcagc tggcgcagta aaagccagac aatcaccgct gtaagaattt
     7501 tatcgaccag gttcgcgcct atcaccgtaa tcgccaccga ctccaccagg ttttgcccca
     7561 tcgagtgcat ccaggcaacg aacaaatcgg ctccgctacc ggtgacaccg ccgaacagct
     7621 cagtacgcag cggtacagcg acgaccgtta ccgcaagcgt aataatcacg ccgctgacaa
     7681 cgacttttgg cagggtgcga aaccaacccg ctcgcgccag ccaaccggcg accaggccga
     7741 tgaccatcgc gacgggtgca aatgcggcgg cgatcgggtc ggtcagcaat ccccagagta
     7801 aattggtcag taagccggtc agcattccga tcaccggacc aagcagtacc gcgctaataa
     7861 gtgtgccgat ggagtcgaga aatataggta atttgaccat gctggccagt tgcccgccga
     7921 tcatattgat ggcgatagat ataacgatca acaccagtgc ctgactggaa aagtggctac
     7981 gcgccataag aaaacctctt tgaaagtagt tatttaacgg gggaaatatg caactcgtcg
     8041 taaccagaaa gcgcgcaagg ttggcggcta aacgccagtt cctcaaggct accgatcact
     8101 agttcaagcg tggtccgcac ggtacagccg ggcatcatat gaccgccaag catcgtgccg
     8161 tgtggatcgg aaacgcagag atgaagatgc tcaccactct gttccagcgt gccgtttagt
     8221 gagatcactt cgaatgtacc gttcaggagc gtggtgcctt cttgcccggc atagcgcaag
     8281 gcgacgtcag tcaggctacc ggtacagcct gcaatccagg cggcatgaag ttgatgctgc
     8341 tgtgcaaaag cgtgtagctg ggaaaaaact tcctgcccgg ggaggaggcg cagtgcgtaa
     8401 aagcgtgcgg aggaagaata tgggtagggc aatgtcatca ttggagtact ccgaaactcg
     8461 gacgtattgc ggataatatc gacgctgcat cagtcatatt ctgatataag tcagggtttg
     8521 tgacggcaca acgaaaagat tttgctacgc ttattcgtga ttctgcaaag tggaataaat
     8581 tccacagggg cggggctgaa aacgtgacgt aagtcagata attgctttct ttgactggac
     8641 aatcattcct tttattccac gtttcgctta tcctagctga agcgtttcag tcgattaaat
     8701 gttcgacaat taaccaatca gtcgcagttt gcgacaggta aggtttcccc ggacgatttg
     8761 ctggattact ctgtctggca aatttgctca gggaaaacct tgcaggagat ctatgaccgt
     8821 acgcgtagcg ataaatggct tcggtcgcat cgggcgtaat gtggttcgtg ctttgtatga
     8881 atcaggacgc cgggcggaaa ttaccgtggt ggcaatcaac gaactggcgg atgctgcggg
     8941 catggcgcat ttgttgaaat atgacaccag ccatggccgt tttgcatggg aagtacgaca
     9001 ggaacgcgat caactttttg ttggtgatga cgccatccgc gtattgcatg aacgttcact
     9061 gcaatcgctc ccctggcgtg aacttggcgt tgatgtagtc ctcgactgca ccggcgtata
     9121 tggctcccgc gagcatggcg aagcgcatat tgccgccggg gccaaaaaag tgctcttttc
     9181 acatcctggc agtaacgatc tcgacgcgac cgttgtttac ggcgtcaatc aggatcaact
     9241 tcgtgcggaa caccgcatcg tttctaacgc ttcctgtacc acgaattgca taattcccgt
     9301 catcaaattg ttagatgatg cgtacggtat tgagtccggc acagtgacca caattcactc
     9361 cgccatgcac gatcaacagg ttattgatgc ataccatcct gacctgcgtc gcacccgggc
     9421 agccagccag tcgatcattc cggtcgatac taaactggcc gccggtatca cacgattttt
     9481 tccgcaattt aacgatcgct ttgaagcgat tgcggtacgt gtgccaacca taaatgtgac
     9541 ggcaatcgat ttaagcgtga cggtgaagaa acctgtaaaa gccaatgaag tcaacctgtt
     9601 gctgcaaaaa gcagcacaag gtgcatttca tggtatagtt gactatacgg aattgccgtt
     9661 ggtctctgta gattttaacc acgatccgca cagtgccatt gtcgatggca cccaaacccg
     9721 ggtcagtggc gcacacctga tcaaaacgtt ggtctggtgc gataacgaat ggggctttgc
     9781 taaccgaatg ctcgacacga cgttagctat ggctactgtt gctttcaggt aagacgcaag
     9841 cagcgtctgc aaaactttta gaatcaacga gaggattcac catgtctgta attaagatga
     9901 ccgatctgga tcttgctggg aaacgtgtat ttatccgtgc ggatctgaac gtaccagtaa
     9961 aagacgggaa agtaaccagc gacgcgcgta tccgtgcttc tctgccgacc atcgaactgg
    10021 ccctgaaaca aggcgcaaaa gtgatggtaa cttcccacct gggtcgtcct accgaaggcg
    10081 agtacaacga agaattctct ctgctgccgg ttgttaacta cctgaaagac aaactgtcta
    10141 acccggttcg tctggttaaa gattacctcg acggcgttga cgttgctgaa ggtgaactgg
    10201 ttgttctgga aaacgttcgc ttcaacaaag gcgagaagaa agacgacgaa accctgtcca
    10261 aaaaatacgc tgcactgtgt gacgtgttcg taatggacgc attcggtact gctcaccgcg
    10321 cgcaggcttc tactcacggt atcggtaaat tcgctgacgt tgcgtgcgca ggcccgctgc
    10381 tggcagctga actggacgcg ctgggtaaag cactgaaaga acctgctcgc ccgatggtgg
    10441 ctatcgttgg tggttctaaa gtatctacca aactgaccgt tctggactcc ctgtctaaaa
    10501 tcgctgacca gctgattgtt ggtggtggta tcgctaacac ctttatcgcg gcacaaggcc
    10561 acgatgtggg taaatccctg tacgaagctg acctggttga cgaagctaaa cgtctgctga
    10621 ccacctgcaa catcccggtt ccgtctgatg ttcgcgtagc aaccgagttc tctgaaactg
    10681 caccggctac cctgaaatct gttaacgatg tgaaagctga cgagcagatc ctggatatcg
    10741 gtgatgcttc cgctcaggaa ctggctgaaa tcctgaagaa tgcgaaaacc attctgtgga
    10801 acggtccggt tggcgtgttc gaattcccga acttccgcaa aggtactgaa atcgtggcta
    10861 acgctatcgc agacagcgaa gcgttctcca tcgctggcgg cggcgacact ctggcagcaa
    10921 tcgacctgtt cggcattgct gacaaaatct cctacatctc cactggcggc ggcgcattcc
    10981 tcgaattcgt ggaaggtaaa gtactgcctg cagtagcgat gctcgaagag cgcgctaaga
    11041 agtaaaaaat cacagggcag ggaaacctgc ccttgtttca gcgcgctttt agagcacgca
    11101 ttttttcata ctctaaataa ttcgagttgc aggaaggcga caagagagtg aatccccagg
    11161 agcttacata agtaagtgac tggggtgagc gaacgcagac gcagcacatg caacttgaag
    11221 tatgacgagt ataaggcccg acgatacagg ataagagaca tgtctaagat ttttgatttc
    11281 gtaaaacctg gcgtaatcac tggtgatgac gtacagaaag ttttccaggt agcaaaagaa
    11341 aacaacttcg cactgccagc agtaaactgc gtcggtactg actccatcaa cgccgtactg
    11401 gaaaccgctg ctaaagttaa agcgccggtt atcgttcagt tctccaacgg tggtgcttcc
    11461 tttatcgctg gtaaaggcgt gaaatctgac gttccgcagg gtgctgctat cctgggcgcg
    11521 atctctggtg cgcatcacgt tcaccagatg gctgaacatt atggtgttcc ggttatcctg
    11581 cacaccgacc actgcgcgaa gaaactgctg ccgtggatcg acggtctgct ggacgcgggt
    11641 gaaaaacact tcgctgctac cggcaagccg ctgttctctt ctcacatgat cgacctgtct
    11701 gaagaatctc tgcaagagaa catcgaaatc tgctctaaat acctggagcg tatgtccaaa
    11761 atcggcatga ctctggaaat cgaactgggt tgcaccggtg gtgaagaaga cggcgtggac
    11821 aacagccaca tggacgcttc tgcactgtac acccagccgg aagacgttga ttacgcatac
    11881 accgagctga gcaaaatcag cccgcgtttc accatcgcag cgtccttcgg taacgtacac
    11941 ggtgtttaca agccgggtaa cgtggttctg actccgacca tcctgcgtga ttctcaggaa
    12001 tatgtttcta agaaacacaa cctgccgcac aacagcctga acttcgtatt ccacggtggt
    12061 tccggttcta ctgctcagga aatcaaagac tccgtaagct acggcgtagt gaaaatgaac
    12121 atcgataccg atacccaatg ggcaacctgg gaaggcgttc tgaactacta caaagcgaac
    12181 gaagcttatc tgcagggtca gctgggtaac ccgaaaggcg aagatcagcc gaacaagaaa
    12241 tactacgatc cgcgcgtatg gctgcgtgcc ggtcagactt cgatgatcgc tcgtctggaa
    12301 aaagcattcc aggaactgaa cgcgatcgac gttctgtaag atattccttt ctgcttatct
    12361 caaggcccgc tctgcgggtc tttttttcgc caaaagcaaa attgcctgat gcgctacgct
    12421 tatcaggcct acacgtatcc tgcaatatat tgaatttgca ggtttttgta ggccggataa
    12481 ggcgttcacg ccgcatccgg catgacaaag cgcacattga tatctgtctg agccccgctc
    12541 tgcggggatt ttttcgccaa aagcaaggtg attcagatga gaaatctgtg atctatttgg
    12601 caaaattatg ctttattgtt tacccttgtc agactgcccg tcataaggcg gcggagtgta
    12661 tttctccatt ttgagtcagt tgaaaaggaa tattgaatgg aagatttgaa tgttgtcgat
    12721 agcataaacg gcgcgggaag ctggctggta gctaaccagg cgctgctgct aagttatgca
    12781 gtaaacatcg tggcggcact cgcgatcatc atcgttggtt tgattatcgc gcggatgatt
    12841 tccaacgcgg tgaatcgcct gatgatctcc cgtaaaatcg atgccactgt tgctgatttt
    12901 ctctctgcat tagtccgtta cggtattatc gcctttacgc taatcgctgc actgggacgc
    12961 gtgggtgtac aaaccgcgtc cgtcattgct gtactcggtg ccgcaggctt agctgttggt
    13021 ctggctttgc aggggtcact gtctaacctg gccgctggcg tgttacttgt catgttccgc
    13081 ccgttccgtg ccggagaata tgttgacctg ggcggcgtag ccggtactgt gctgagtgtg
    13141 cagattttct ccaccaccat gcgtactgca gacggtaaaa ttatcgttat tccgaacggt
    13201 aaaattattg ccggaaatat tattaacttc tcccgcgagc cagttcgccg taacgaattt
    13261 attattggcg tggcgtatga ttccgatatc gatcaggtta agcagatcct gaccaatatt
    13321 atccagtctg aagatcgcat tttgaaagat cgcgaaatga ctgtgcgcct gaacgaactt
    13381 ggtgcatcgt cgattaattt cgtggtccgc gtctggagca acagcggcga tctgcaaaac
    13441 gtgtactggg atgtgctgga gcgtattaaa cgtgaatttg atgccgccgg tatcagcttc
    13501 ccgtacccgc aaatggatgt gaactttaag cgggtgaaag aagacaaagc tgcgtaatca
    13561 acgctgaggc cagataatac tctctggcct ctctcttatt agttattctg attgccaatt
    13621 aatattatca atttccgcta ataacaatcc cgcgatatag tctctgcatc agatacttaa
    13681 ttcggaatat ccaacgtgtt ttcttattac tttcaaggtc ttgctcttgg ggcggctatg
    13741 atcctaccgc tcggtccaca aaatgctttt gtgatgaatc agggcattcg tcgtcagtac
    13801 cacattatga ttgccttact ttgtgctatc agcgatttgg tcctgatttg cgcggggatt
    13861 tttggtggca gcgcgttatt gatgcagtcg ccgtggttgc tggcgctggt cacctggggc
    13921 ggcgtagcct tcttgctctg gtatggtttt ggcgctttta aaacagcaat gagcagtaat
    13981 attgagttag ccagtgccga agtccttaag caaggcagat ggaaaattat cgccaccatg
    14041 ctggcagtga cctggctgaa tccgcatgtt tacctggata cttttgttgt actgggcagc
    14101 ctcggcgggc aacttgacgt cgaaccaaaa cgttggtttg cgctcgggac aattagtgcc
    14161 tctttcttgt ggttctttgg tctggctatt ctcgcagcct ggctggcacc gcgtctgcgc
    14221 acagcaaaag cacagcgcat tatcaatctg gtagtgggat gtgttatgtg gtttattgcc
    14281 ttgcagctgg cgagagacgg tattgctcat gcacaagcct tgttcagtta ggcgctatct
    14341 gatggaaaaa taaaacagag gcgctaagct tgcctccaga ggtcctgaat tttgggcaag
    14401 tcaattaaca cggagagact aacgtgaagt tcaaagttat cgccctggcg gcattaatgg
    14461 gtattagcgg gatggcagcg caggctaacg aattgccgga tggaccgcat attgtcacct
    14521 ccggtacggc aagcgtggat gcggtgccag acattgccac tcttgcgatt gaagtcaacg
    14581 tggccgcgaa ggatgccgct actgccaaga aacaggcgga tgagcgcgtc gcacaataca
    14641 tttccttcct tgaactcaat cagatcgcga aaaaagatat cagctcagcg aatttacgca
    14701 cccagccaga ttacgactat caggatggta aaagtatcct gaaaggctac cgcgctgtga
    14761 gaacggtgga agtcacgctc cgtcagttag acaaactgaa ttccttgctg gatggcgcgc
    14821 tgaaggcggg tcttaacgaa attcgttctg tgtcgctggg cgtggcgcag ccggatgcct
    14881 ataaagacaa agcgcgtaag gcagcgattg ataacgcgat tcatcaggcg caggaactgg
    14941 cgaacggctt tcatcgtaaa ctggggccgg tatatagcgt gcgctaccat gtttccaact
    15001 atcagcccag cccaatggtg cggatgatga aagccgatgc cgcgccggtg tctgcccagg
    15061 aaacttacga gcaggccgct attcagtttg atgatcaggt cgatgtggtc ttccagttag
    15121 aacctgtgga tcaacaaccc gctaaaacac ctgcagcaca ataatacttg tagcccgacg
    15181 tattcgtcgg gctaatgcct ttacccgata ttgcctcctt tttctgtaat tttctgtaaa
    15241 accatgacgt aatcacctat ctttgctcgc ggtttcatca aggataggct gactgaaacg
    15301 gttaagcgga caaggatgcg gggagggggg atggacattt ttatttcgaa aaagatgcgc
    15361 aatttcattt tattagcgca aaccaataat attgcccggg cggcagaaaa aatccatatg
    15421 acggcttcgc catttggtaa aagcattgcc gcgctggaag aacaaattgg ctatacgcta
    15481 tttacccgca aagataataa tatcagcctc aataaagccg gtcaggaact gtatcaaaaa
    15541 ctgttcccgg tttatcaacg actttctgcg attgataatg aaatccataa ctcagggcgt
    15601 cgttcacgag aaattgtgat tggcatagat aatacttatc cgacaattat tttcgatcag
    15661 ttgattagtc tcggagataa gtatgaaggc gtgaccgccc agccagttga gttcagcgaa
    15721 aacggcgtta tcgataatct ttttgaccgt cagttagatt ttattatctc cccacagcat
    15781 gtatctgcac gagttcagga actggaaaac ctgactatca gtgaattacc accgctgcga
    15841 ttgggctttc tcgtttctcg ccgttatgaa gagcgtcaag agcaagagct gcttcaggag
    15901 ctgccctggc tacaaatgcg ctttcaaaac cgggctaact tcgaagcaat gattgatgcg
    15961 aatatgcgac cttgtgggat caatcccacc attatttatc gtccctacag ttttatggcc
    16021 aaaatcagcg ccgtggagcg cggacatttt ctgacggtga tcccgcattt tgcctggcga
    16081 ctggtgaatc cggcaacgct gaaatatttt gatgcgccgc acaggccgat gtatatgcag
    16141 gaatatcttt attcaatcag aaatcatcgt tataccgcca cgatgcttca gcatattgct
    16201 gaagatcgtg acgggacgag tcattaaccc agcatcgagc cggttgcaat taaattacgg
    16261 tgtaagtcga agacgtggct aagatcgtgg tgaatatgtc cgccaggcgc attttccaga
    16321 tagcgatgca gataatcccg atacatagga tgtgcacaat tatcaatgat agtgcgggcg
    16381 cgttgaagcg gggaaagacc gcgcagatcg gcgatccctt gttcggtgat gatcactttg
    16441 acgctgtgtt cgctgtgatc aacatggctg cacattggca cgacggttga gatcttgcct
    16501 tctttagcaa tcgacggggc cataaagatc gacagatacg cgttgcgttc aaaatcaccg
    16561 ctgccgccga tgccgttcat cagatcgacc ccggctacgt gcgttgagtt ggcatgcccg
    16621 taaatatcaa actccaggcc gacgttcaga gcgatgacgc ccagacgacg gatgatttcc
    16681 gggttattgg aaatctcctg cggacgcaac acaatgcggc tggcaaagta atccatattg
    16741 tcgtaaatct tgcgcaggga atcggccgag attgtcaggc tggaggcgct ggccccgctg
    16801 attttgccgg tttccagtaa atgcaccacc gattcctgta gcacttccga atacatcata
    16861 aacggaggaa tttccgggtt ttcccccaga cgcgccatta ccgcattatt gatattgccc
    16921 acgccacttt gcagcggcag aaattccggc ggaatacgcc catgcgccat ttcctgcaat
    16981 aagaacgtga ccacgttatc ggcaatctgc tggcacatgg gattttgctt atccagcata
    17041 ttaccggcgt cgggcaagtt ggtttccacg acggcgacaa tctttttcgg atcgatttgc
    17101 acatagcggg taccgacgcg atccattgca tggaagatcg acacgctatt gcgccgcggt
    17161 ggcgcgccag gaatcacaat atccgccagt tctgcaacgc gcggatcgtg atagtgatta
    17221 agttcaatga tcactttctt cgcccgcaga agccatgtcg gcgcattacc gatcccgctg
    17281 gttaaccaga ctcgaccatc cggtgccagt gccgatgctt cgatgacggc aacatcaatg
    17341 tcgccgaaga aaccgtaatt gaccatttgc gccacttcgc tcaaatgcag gtcaacgaaa
    17401 ctcaccgcgc cctgattgat ctttttacgt aaaccggacg atgtttgata tggcgcacgc
    17461 caggaaacag catcggcgtc agaaagtaca tcgtcagcgg cggcgctgat tgacgccccc
    17521 gtcagcaggc gaatttgata cggctttttg gcctcatgct gttcgttagc tctgcgggca
    17581 atcgcggtgg gtagggcttt cggcgaaccc gccggggtaa agccgctaaa tgccaccatg
    17641 tcgttatgct ggataatttc tgccgcttca tcggcggtca tccttgtcca ctgagtttcc
    17701 atttttactc cttcgttcgc agggattaat gaccaacgaa attaggttta cgtttttcga
    17761 ggaaagcgtt cattccttcc tggtaatctt cgctgtcata aaccgcgcgt cgcatcccct
    17821 gaatacgttc aaattcatcg gagttcatgg tgtgcgcttc gcccagtaca cgcagctctt
    17881 ctttgataac agcaatggct aacggcgctt tctcagagat gtggtgcgcc atttgtaagg
    17941 tgaaatcttc cagttcttcc acttccacaa catggttgag gatgccgaca gccaacgcgc
    18001 gctgggcggt gattggcgaa gcggtaaaaa tcagctcttt gacaatgtgg aagcccgcgt
    18061 cgcgggtcag gttgtgaatg ccgaccaggt tatacgggac gccgaggttt acaggcgtca
    18121 ttgagaaggt tgaggtactg gcggcgataa tcagatcgga actcatgatc atttcaaatg
    18181 cgccacccca aacactacct tccaccatcg aaatgatcgg tttcgggaat ttttggatca
    18241 tgcgggtgat ttgacgcaat ggatcatcat aggagagcgg atcgcgaccg ccagacggca
    18301 gttcgtgaat atcgtggcct gcggagaaga ctttggatcc actcggcgcg cgcaaaatga
    18361 tacagcgaat ttccggccgg ttgagatcgc ttaatgcctg cataagatca tcaataaaga
    18421 ctttacttaa ggcattaagt ttgcggccat agttaaactc aatgaccgcc actttgttga
    18481 tagtgacaac gttaacatac tgataagaca taaaaattcc tttaatcaaa atattgcgtc
    18541 tggataaatt cactgagctg ccgcaggccg gtgcgcggtg agagcgtatt gtttttgacc
    18601 gctaaaagcg tctggcggta atagcgatcg aaatcttcat tcgcgaacag gtgatgcagt
    18661 acttcttctt cggtctgctt acgcagccat tccaccgatt gttgttgccg cacttgttgt
    18721 aaacgaccac tggcagttag cgcggttttg aagtcgatga tggcgtgcca gatctcatcg
    18781 attccacgtt tttccagtgc gctacaagtc agaacccgtg gctgccattc gtcgtattta
    18841 cgtcgcagaa tatgcagggc actctcgtac atatgccggg caatggcgac attggtatgg
    18901 ttatcgccat cgtctttgtt gataacgagc agatcagcca cttccatcag ccctttttta
    18961 atgccctgca gatcatcgcc gccaccggca atttgcaacg agataaaaca gtccaccatg
    19021 cgggcgactt ccgtttccga ctgcccgacg ccaaccgttt cgacaatcac tacgtcataa
    19081 cccgccgctt cgcataacag cattaattcc cgcgctcgct gactggcacc gcccagatga
    19141 ccggaggatg gtaccgggcg aataaacgcc gcttcggcac gcgccaggtc attcatgcgg
    19201 gttttatccc cgagaatgct accgccagtg accgggctgc tgggatcgac cgcaataacc
    19261 gcgaccttta atccctcacg aatcaacaac atgccaaagg cttcaagaaa ggtacttttc
    19321 cccgcgccgg gtgtgccggt aacgcccagt cgcagggcgt taccgcagta cggcataatg
    19381 gcatcaagca gttgcgtact tagcgcctga tgacgcgggt gacggctttc caccagcgtc
    19441 atggcctggg cgagtgtggc acgctcaccc tgacgtaagc ggcgaatact ttctgccagc
    19501 gtggcttcat taatcatgat gctggcttat cagattcagt acgtcgcgca cactctcgag
    19561 cataggtgta cctggaccat aaatcgccgc cacgccgcgc tcttgcagga aggcgtaatc
    19621 ctgcggtgga atgacgccac cggcgactac gcagatatct tcgcgtcccc attttttcag
    19681 cgcttcgacc agttccggga tcagcgtttt atgaccggca gccagtgagg atgcgcccac
    19741 tacgtgaacg tcgttttcta cggccaggcg ggcgatctct tcaggtgtag agaacatcgg
    19801 gcttaaatct acgtcgaaac cgagatcgga ataggcgctg gcgatcactt tcgcgccgcg
    19861 atcgtgtcca tcctggccca tcttagcgat cagaatgcgc gggcgacgac cattgtcggc
    19921 aaggaactgc tccgtttgcg caacaatggc atcgaactcg gaggccgatt tctcagactg
    19981 atgatagctt tgcgcaatca cgccggtaac acactggctt ggcaccagat aacggtcgaa
    20041 agcgacttcc agcgcatcgg aaatttcacc cagggtggcg cgaacgcgag cggcattaac
    20101 agcggcagcc agcaggtttt cgttatgctg tgcggcgtga gtcagggcgt tcaacgcggc
    20161 ggttacggcg gcatcatcac gggtggcgcg aatgcgttcc agcgaagcaa tttgctcgtt
    20221 acgcaccatc acgttgtcga tctcaagtac atcggtttcg tcttcgtgat ccagtttgta
    20281 cttgttgaca ccaacgatga cacgcttgcc ctggtcgatc agcgactgtt cgcgcgctga
    20341 ggcctcttcg atcattcgtt ttggcagacc tgcttcgatc gctttcgcca tgccaccggc
    20401 ttcgtcgatc tattggataa tagctctggc ttgtttgacg atttgatcgg tcagcgactc
    20461 aatgtaatag gatccggcca gtggatcgac ggtgcggcag agttctgatt cttcctggat
    20521 gatgatctgg gtgttgcggg caatgcgtgc tgagaaatcg gtaggcaaac caagcgcttc
    20581 gtcaaaggcg ttggtatgca gtgactgagt accgcccagc gtcgcagcca gcgcttcaat
    20641 ggtggtgcgg ataacgttgt tatacggatc ctgttcagtc aggctccagc ctgaggtctg
    20701 gcagtgggta cgcagcgcca gtgatttcgg gtcctgtgcg ccaaatccac tgacctcttc
    20761 gctccataaa taacgtgccg cacgcaacat ggcgacgttc ataaacagat ccatgccgat
    20821 gccgaagaag aacgacaggc gaggagcgaa gtcatcaatt ttcagtccgg cagagattgc
    20881 tgctttgatg tactcaatcc catcagcgag cgtaaatgct acctgctgca cgcagttggc
    20941 acccgcttca cccatgtggt aaccgctgat actgatggta ttaaatcgcg gcatgttgcc
    21001 ggaacaccag gcgatgatgt cggcgataat gcgcattgac ggttttggcg ggtaaatata
    21061 ggtgttgcgg cagaggtact ctttgagaat atcgttttga atggtgccgg tcagtttatc
    21121 aggtgtaaca ccttgctctt ctgcggcgac gatataaaac gccagtactg gtagcactgc
    21181 gccattcatg gtcatcgaaa ccgacatttt atccagcggg atctggtcga acaggacttt
    21241 catatcttcc acggtgtcga tagcgacgcc cgctttgccg acgtcacccg ccacgcgtgg
    21301 gttatcggag tcgtagccac ggtgggtggc aaggtcaaac gcaacggaaa gacctttttg
    21361 cccggcggcc aggttacggc gataaaaagc gttggactct tttgctgttg aaaaaccagc
    21421 atactgacgg atggtccacg gttgggcggt atacatagtg gcacgcgggc cacgaacgta
    21481 gggcggcaaa ccaggaaggg tacctgtcac ctccagatta tcgagatcgg cttcggtata
    21541 cagcggcttg atggcgatcc cttccgcggt ttgctgaacc agcgagtcga cagttttctc
    21601 ccgacggctc aattccttgt tggcaagctg ttgccactcc tgctcgttag acattatttg
    21661 ctccgtaaaa cgcaatcaaa atgagggtat ttgcgcttac ggtgagcttt ttacgtgggt
    21721 ttgtcgccag caaaaaaaat gcattcagac gtcgtttttt gccgcctgag atttgtctat
    21781 tttgtgatgt gattcaagga atacaaaaag aagtgaggac aacgcctgat atgtgctatc
    21841 aggcattatt tgatggatta atcctgacga aggactttgt gaccataatc gagtaacgca
    21901 tcagtgactt tacgcatcat gcggctttca ggagcaaagc ggtgccagta gagcatccgt
    21961 cgttgaaata gcccaggcgt taagtcaatc agttcaccgc tggccagctc tttctcgatt
    22021 tgcaggtgcg ggatcataca gcaggtggtg ccctggcgag caagttgtac gaacgcttct
    22081 gaagaattaa cgatatggca gggcacgctg cctggaggca gatcgaagtt ttgctgcaaa
    22141 aaggcctggt gcatatcgtc aagatggtca aacgcgacca ctggcgcttt cagtaatgcc
    22201 gaacgcgtta cgccgttagg gaaatatttt tcggcaaagg gttttgagct gacgaacaga
    22261 tagtcgagcg caccaagttt atcgacaaga caactcggca gcgcctgatg ttgaatactc
    22321 accgcgccga ccacttcgcc gcggcgcaga cgttcctgag tgcgggtttc atcttctacc
    22381 tgcaagttga ggcggatagg cgaatcagcc aacacaggag ccagtgcagg aagcaaccac
    22441 gtcgccagac tgtcggcgtt gaccgccagt gaaagcagca gcggagtcga accggtttgt
    22501 tcatcgccca gccactcttc ttccagcaac tccacctggc gcagcagtgc cagcagtttt
    22561 tgcccttgtt ccgtcgggcg cggcggtacg gtacgcacca acagcggctg cccgaacata
    22621 ttttccagtt gcttaatgcg ctgtgagacg gctgattgtg taatgcacag cttttgtgcc
    22681 gcgcgctcaa atcctcgttc acgtatcacc gcatccagtg cctgtaatgt tctgtagtcc
    22741 gggcgtttca ttgctgttgt ctgctcctgg taaatgcttt ttcctgcact atgacacaat
    22801 tttgtgtcag gttgcatata ccgaagccgt cggcgggtaa catttgtatt cgtggttaaa
    22861 cgcgcagctt aaaagatcag ggatttgcgc taatgcattt cgctcagcga gttcgcgccc
    22921 tggttgtgtt gaatggcgtg gcgttattgc ctctgttcgc accgtggagc ggcataccca
    22981 gaccgttgta tgctttattt gcggggcgaa aggggatgcc cgccattgcg cgatatttta
    23041 tggatgagtt aaccacgcgg cttgccaacg gggtctgaat cgcttttttt gtatataatg
    23101 cgtgtgaaat ttcataccac aggcgaaacg atcatgacgc aggatgaatt gaaaaaagca
    23161 gtaggatggg cggcacttca gtatgttcag cccggcacca ttgttggtgt aggtacaggt
    23221 tccaccgccg cacactttat tgacgcgctc ggtacaatga aaggccagat tgaaggggcc
    23281 gtttccagtt cagatgcttc cactgaaaaa ctgaaaagcc tcggcattca cgtttttgat
    23341 ctcaacgaag tcgacagcct tggcatctac gttgatggcg cagatgaaat caacggccac
    23401 atgcaaatga tcaaaggcgg cggcgcggcg ctgacccgtg aaaaaatcat tgcttcggtt
    23461 gcagaaaaat ttatctgtat tgcagacgct tccaagcagg ttgatattct gggtaaattc
    23521 ccgctgccag tagaagttat cccgatggca cgtagtgcag tggcgcgcca gctggtgaaa
    23581 ctgggcggtc gtccggaata ccgtcagggc gtggtgaccg ataatggcaa cgtgatcctc
    23641 gacgtccacg gcatggaaat ccttgacccg atagcgatgg aaaacgccat aaatgcgatt
    23701 cctggcgtgg tgactgttgg cttgtttgct aaccgtggcg cggacgttgc gctgattggc
    23761 acacctgacg gtgtcaaaac cattgtgaaa tgatctgacg ggggaacctc ccccgttaaa
    23821 aaaattctct tcattaaatt tggtgacatg tgtcacgttt ttacctggct attgtcgatt
    23881 gctctaaata aatcttctaa accagcatat tcatccaaga attacctttg cgtgatattt
    23941 cctcaacatc gcgacgcaaa cgttcatatt gccgcaatat tattttttga tatgttgaaa
    24001 ggcggacgca aatccgcaca caacatttca aaagacagga ttgggtaaat ggcaaaggta
    24061 tcgctggaga aagacaagat taagtttctg ctggtagaag gcgtgcacca aaaggcgctg
    24121 gaaagccttc gtgcagctgg ttacaccaac atcgaatttc acaaaggcgc gctggatgat
    24181 gaacaattaa aagaatccat ccgcgatgcc cacttcatcg gcctgcgatc ccgtacccat
    24241 ctgactgaag acgtgatcaa cgccgcagaa aaactggtcg ctattggctg tttctgtatc
    24301 ggaacaaacc aggttgatct ggatgcggcg gcaaagcgcg ggatcccggt atttaacgca
    24361 ccgttctcaa atacgcgctc tgttgcggag ctggtgattg gcgaactgct gctgctattg
    24421 cgcggcgtgc cggaagccaa tgctaaagcg caccgtggcg tgtggaacaa actggcggcg
    24481 ggttcttttg aagcgcgcgg caaaaagctg ggtatcatcg gctacggtca tattggtacg
    24541 caattgggca ttctggctga atcgctggga atgtatgttt acttttatga tattgaaaat
    24601 aaactgccgc tgggcaacgc cactcaggta cagcatcttt ctgacctgct gaatatgagc
    24661 gatgtggtga gtctgcatgt accagagaat ccgtccacca aaaatatgat gggcgcgaaa
    24721 gaaatttcac taatgaagcc cggctcgctg ctgattaatg cttcgcgcgg tactgtggtg
    24781 gatattccgg cgctgtgtga tgcgctggcg agcaaacatc tggcgggggc ggcaatcgac
    24841 gtattcccga cggaaccggc gaccaatagc gatccattta cctctccgct gtgtgaattc
    24901 gacaacgtcc ttctgacgcc acacattggc ggttcgactc aggaagcgca ggagaatatc
    24961 ggcctggaag ttgcgggtaa attgatcaag tattctgaca atggctcaac gctctctgcg
    25021 gtgaacttcc cggaagtctc gctgccactg catggtggtc gtcgtctgat gcacatccac
    25081 gaaaaccgtc cgggcgtgct aactgcgctg aacaaaatct tcgccgagca gggcgtcaac
    25141 atcgccgcgc aatatttaca aacttccgcc cagatgggtt atgtagttat tgatattgaa
    25201 gctgacgaag acgttgccga aaaagcgctg caggcaatga aagctattcc gggtaccatt
    25261 cgcgcccgtc tgctgtacta attccccttc tctgaaaatt aacgggcagg tcactgactt
    25321 gcccgttttt ttatcccttc tccacaccgt tgccgcgttg cgcattctac tttgcgagtc
    25381 tgctccagaa aataaatcaa agtcagatca atgcgttgtg tttatttaat ggggatttac
    25441 gatggcggga cagcatgggg ctgtaacggt aaagccctca ccgaagcgag ggcttaaagg
    25501 agaagggtta tgatgcgact tgtcatcata ctgattgtac tgttactgat aagtttcgga
    25561 gcttattaac agtcagtctc aggggaggag tcatcctccc ttacccttac tcactaaatt
    25621 aggtcaaaga atcaacgatg tcaatcaggg cgatgcgggg tgcatcgccc tgaaccgaac
    25681 tttttgatag ccacttcatc gcccacgttt actgccgtaa tagatccttt ttcccaaaaa
    25741 ttatctctgt tttgcatggg gtttttcgag cacgctcgaa gaaaagattt aaaggtagat
    25801 caatgcgttg tggttattta aagagagctt acgatagctg ggcagcatgg ggctgtaacg
    25861 gtaaagccct caccgaagcg agggcttgaa ggagaagggt tatgatgcga cttgccatca
    25921 tactgattgt actgttactc ataagtttcc cagcttatta acagtcagtc acaggggagg
    25981 agcaatcctc ccttaccctt actcactaaa ttaggttaaa gaatcaacga tgtcaatcag
    26041 ggcgatgcag gtgtatcgcc cttaccactc ccagactttc gacggtgtaa ccaccgcagg
    26101 aagagggata tcccactctt caacggggag tttttccacc aactgacaat catgcgcata
    26161 acccaccggt tgcgttttat agtgctgcca gttttgtaag gtccgatcat aaaaaccacc
    26221 gcccattccc aggcgctgac cgtactcatc aaaggcgacc agcggtgtga tcagcacgtc
    26281 taatcgggaa aggggtagca cgtcacgcac atccagtttt ggctcatgga tcttcaacct
    26341 gttcatcacc agttcgcttt gcggatggta attcaggaac agcaaattac cggcactaaa
    26401 gggatgcaaa actggaagat atacgcgctt accggcgcgc cagagttgtt ctattagcgg
    26461 ctgggtgtcg agttcaccat caaaagagag gaatacagcg accgtatgcg ccatcaccac
    26521 cgggggataa gtcatcatcc gggtagcggc ttgttgaccc atttcctgct gttgttccgg
    26581 cgttaacgca cgacgacgtt gccgaatcat tttgcggatt tcttgtcggg ataatgtcag
    26641 tgggagttct ggtagttgcg tcatggctgg tgccagataa gaagggaatc tccgagatgc
    26701 cgccgcaggc tgtaaccctt gaacccttgg ttcaaggtga atgtgtcgtc gcagttttaa
    26761 ggcttctcgg acggaccgag catgctcacc aaccgcggag cgccacattc ttgtggtatg
    26821 aaatatcggc tcaggggact ggcccgcttg cgaacatctc agagaaattt tgtcttcacg
    26881 gttactctac cacagtaaac cgaaaagtgt cattcaaagt tttggttagt tttttcggtg
    26941 atgcgacctt gttcaagtaa cgcttgttct atggtctgct gcagcatccg aatacgctgt
    27001 tccatacttg ccgcgtagtc acgagtcttc gctttttctt gcgctaactc atagctgata
    27061 ttcaatgcgg caatgaagac caactgttca gtatttgtga ctctagtgcg ttctttcaga
    27121 tcttgcaacc gttggttcag atcgtccgct gcctgattca acgcatccct ttggtcaggc
    27181 gggcagttca cacgcagtga acggccaaaa atttggatat cgacgggttg tgcagacatg
    27241 ccaccttcct gctgattgac tgcgctgcct tcgtctttag acccatggtc tgcgaagggg
    27301 cgacactata gctaccctga tgagaagaga caagcccttt tctggtccac cagggcccaa
    27361 agtggtagca tatcatgaat attcctccct ttgacgacga atgcttatgt ctatacagaa
    27421 cgaaatgcct ggttacaacg aaatgaacca gtatctgaac caacaaggga cgggtctgac
    27481 ccctgctgag atgcatggtt taatcagtgg gatgatatgt ggcggtaacg atgacagctc
    27541 atggctaccg ctacttcacg acctgacgaa cgaaggcatg gctttcggtc atgagctggc
    27601 acaggcactg cgtaaaatgc actctgccac cagcgatgcc ctgcaggatg acggcttcct
    27661 ttttcagctt tatctgcctg atggcgatga tgtcagcgtt ttcgatcggg ctgatgcatt
    27721 ggcaggttgg gtcaatcact tcctgcttgg tcttggcgtt acgcaaccga agctggataa
    27781 agtgaccggc gaaaccggtg aagctatcga cgatctgcgt aacattgcgc aactgggtta
    27841 cgacgaagac gaagatcagg aagagcttga aatgtcgctt gaagagatca tcgaatacgt
    27901 tcgtgttgcc gcgctgttat gccacgacac ctttactcat ccgcaaccga ccgcgccaga
    27961 agtacaaaaa ccgactctac actaaaaaca aaaaacgtaa ggagagtgtt atgagtgaga
    28021 tatcccggca agagtttcag cgtcgccgtc aggccctggt ggagcaaatg caacccggca
    28081 gcgccgcgct gatttttgct gcaccagaag taacacgtag cgccgacagc gaatacccct
    28141 atcgtcagaa cagtgacttc tggtacttca ccggctttaa cgaaccggaa gcggtgctgg
    28201 tgctgattaa aagcgatgac actcataacc acagcgttct gtttaaccgc gttcgcgacc
    28261 tgacggcgga gatctggttt ggccgtcgtt taggtcagga tgccgcgcca gagaaactgg
    28321 gcgttgaccg cgcactggca ttcagcgaaa ttaatcagca actttatcaa ttacttaatg
    28381 gtctggatgt ggtttaccac gcccagggcg aatataccta tgctgatgaa atcgtgaaca
    28441 gcgcgctgga aaagctgcgt aaaggttcgc gacaaaatct caccgcgccg gcaacgatga
    28501 tcgactggcg tcccattgtt catgaaatgc gcctgttcaa atcgccagaa gagattgccg
    28561 tacttcgccg cgcgggtgaa atcaccgccc tggcgcatac ccgggcgatg gaaaaatgcc
    28621 gtccgggaat gttcgagtac cacctggaag gcgaaattca ccacgaattt aaccgccacg
    28681 gcgcgcgcta tccttcctat aacaccattg tcggcagcgg tgaaaacggc tgcattttgc
    28741 actacaccga aaacgagtgt gaactgcgtg acggtgacct ggtgttgatt gacgcgggtt
    28801 gtgaatacaa aggttacgct ggcgatatta cccgcacctt cccggtcaac ggcaaattca
    28861 ctcaggccca gcgtgaaatc tacgacattg tgctggagtc tctcgaaacc agcttgcgcc
    28921 tgtatcgtcc gggaacttcc atgcaggaag tcaccggtga agtggtgcgc atcatggtta
    28981 gcggcctggt aaaactcggc attctgaaag gtgatgttga tgaactgatc gctcagaacg
    29041 cccatcgtcc tttctttatg catggcctta gccactggtt agggctagat gtccatgacg
    29101 ttggcgttta tggtcaggat cgctcgcgca ttctggaacc gggcatggta ctgaccgtag
    29161 agccagggct gtatattgcg ccggatgcag aagtgccaga acaatatcgc ggtatcggca
    29221 ttcgtattga agacgacatt gtgattaccg aaaccggtaa cgaaaacctc accgccagcg
    29281 tggtgaaaaa gccggaagaa atcgaagcgt tgatggcagc tgcgagaaag caatgagcgt
    29341 aatcatcgtc ggtggcggca tggcgggcgc gacgctggcg ctggctattt cccggttaag
    29401 tcacggggcg ctgccggtac atttgattga agcgaccgcg ccagagtcac atgctcatcc
    29461 gggctttgat ggacgagcta ttgcgctagc ggcggggacc tgtcagcaac tggcgcgcat
    29521 cggcgtctgg caatctctgg ctgattgcgc aaccgccatc actaccgtgc atgtcagcga
    29581 tcgtggtcat gctggattcg tcaccctcgc cgcagaagat taccaactgg cggcgctggg
    29641 gcaggttgtc gaattgcata atgtcgggca acggctgttt gcattgctgc gaaaagcacc
    29701 tggcgtaacg ctgcattgcc ctgatcgcgt agctaacgtt gcccgtactc agagtcacgt
    29761 tgaagtgacg ctggagagcg gcgagatgct gacgggccgc gtgctgatag ccgctgatgg
    29821 cacccattca gcgttagcca ccgcgtgcgg cgttgattgg cagcaggagc cttacgaaca
    29881 actggccgtg attgccaacg ttgcgacttc cgttgcgcac gaaggccgcg cttttgaacg
    29941 cttcacgcaa catggcccgc tggcgatgtt gccgatgtct gacggacgct gttcgctggt
    30001 ctggtgtcat ccactggaac ggcgcgaaga ggtgctgtcg tggagcgacg agaagttttg
    30061 ccgtgaactc cagtcggcct ttggctggcg acttggaaaa attacccacg ccggtaaacg
    30121 cagtgcttat ccgctggcgt taacccgcgc cgccagagct attacccatc gtaccgtgct
    30181 ggtgggcaat gcggcgcaaa ctctgcaccc gattgccggg caagggttta acctcggtat
    30241 gcgagatgtc atgagccttg cggaaaccct aactcaggcg catgagcgcg gagaagacat
    30301 cggtgattac ggcatattgt gccgctatca acagcgtcga cagagcgatc gcgaagcaac
    30361 catcggcgtc acggacagcc tggtacatct ttttgccaac cgttgggcac cgctggttgt
    30421 cgggcgcaac atcggactga tgacgatgga attattcacc ccggcacgcg atgtgctggc
    30481 gcagcgcacc ctcggttggg tggcgcgttg aaggcggttt taaggaataa acaatgcaaa
    30541 gtgttgatgt agccattgtt ggtggcggca tggtggggct ggcggttgcc tgcggcttac
    30601 aggggagcgg cttacgcgtt gccgtactgg agcagcgcgt accggaacct ctggcggcgg
    30661 atgcaccacc acaactgcgc gtttcggcta tcaatgccgc cagcgaaaaa ttactcaccc
    30721 gtcttggcgt ctggcaggac attctctctc gtagggccag ttgttatcac ggtatggaag
    30781 tgtgggacaa agacagtttt ggtcacattt cgtttgacga tcaaagcatg ggctatagcc
    30841 atcttgggca tatcgttgaa aactcagtga ttcactacgc gttgtggaac aaagcgcagc
    30901 agtcgtcaga tatcactctg ttggcccccg cagaattaca gcaggtcgcc tggggagaaa
    30961 atgaaacttt cctgacgctg aaagatggca gcatgttaac ggcgcgtctg gtgattggcg
    31021 cggacggtgc taattcctgg ttgcgcaaca aagcagatat tccgctgact ttctgggatt
    31081 atcagcatca cgcgctggta gcgaccattc gcacggaaga accgcatgat gcggtggcgc
    31141 ggcaggtttt ccatggcgaa ggcattctgg cctttttacc gcttagcgat ccgcatcttt
    31201 gctcaattgt ctggtcactg tcgccagagg aagcgcagcg gatgcagcag gcaagtgaag
    31261 atgaatttaa tcgcgcgtta aatatcgctt ttgataatcg cctgggctta tgcaaggttg
    31321 agagcgcgcg tcaggtgttc ccactgacgg ggcgttatgc gcgccagttt gccgcgcacc
    31381 gtctggtgct ggtgggcgac gccgcgcata ccattcaccc gctggcgggg cagggagtaa
    31441 atctcggctt tatggatgct gcagagctga ttgctgaact gaaacggttg catcgtcagg
    31501 ggaaagacat cgggcagtac atttacctgc gccggtatga gcgtagccgc aagcacagtg
    31561 cggcgctgat gctggctggt atgcagggat tccgcgatct gttttccggt accaatccgg
    31621 cgaaaaaact gctgcgtgat attggtttga aactggccga cacgcttcct ggcgttaaac
    31681 cacaacttat ccgccaggca atgggattaa acgatttgcc tgaatggctg cgttaaaaat
    31741 ttctcttctg ctgtttattt gatacccatc acactttcat ctcccggttt tttcgccggg
    31801 agattttcct catttgaaat aaactaattt cacctccgtt ttcgcattat attttctaat
    31861 gtcattattt tttgattta
//
LOCUS       DAZGVQ010000043        31754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000043 DAZGVQ010000000
VERSION     DAZGVQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.043"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(42..929)
                     /gene="rarD"
                     /locus_tag="ACHLAG_001928"
     CDS             complement(42..929)
                     /gene="rarD"
                     /locus_tag="ACHLAG_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFP0655169.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAISAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(981..1448)
                     /gene="yigI"
                     /locus_tag="ACHLAG_001929"
     CDS             complement(981..1448)
                     /gene="yigI"
                     /locus_tag="ACHLAG_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFP0655170.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1613..2482
                     /gene="pldA"
                     /locus_tag="ACHLAG_001930"
     CDS             1613..2482
                     /gene="pldA"
                     /locus_tag="ACHLAG_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFP0655171.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2615..4444
                     /gene="recQ"
                     /locus_tag="ACHLAG_001931"
     CDS             2615..4444
                     /gene="recQ"
                     /locus_tag="ACHLAG_001931"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP0655172.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4508..5128
                     /gene="rhtC"
                     /locus_tag="ACHLAG_001932"
     CDS             4508..5128
                     /gene="rhtC"
                     /locus_tag="ACHLAG_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFP0655173.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5190..5810)
                     /gene="rhtB"
                     /locus_tag="ACHLAG_001933"
     CDS             complement(5190..5810)
                     /gene="rhtB"
                     /locus_tag="ACHLAG_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFP0655174.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5921..6943
                     /gene="pldB"
                     /locus_tag="ACHLAG_001934"
     CDS             5921..6943
                     /gene="pldB"
                     /locus_tag="ACHLAG_001934"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFP0655175.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6951..7751
                     /gene="yigL"
                     /locus_tag="ACHLAG_001935"
     CDS             6951..7751
                     /gene="yigL"
                     /locus_tag="ACHLAG_001935"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFP0655176.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7827..8726
                     /gene="bioP"
                     /locus_tag="ACHLAG_001936"
     CDS             7827..8726
                     /gene="bioP"
                     /locus_tag="ACHLAG_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFP0655177.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQLHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8614..9567)
                     /gene="metR"
                     /locus_tag="ACHLAG_001937"
     CDS             complement(8614..9567)
                     /gene="metR"
                     /locus_tag="ACHLAG_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFP0655178.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9685..11946
                     /gene="metE"
                     /locus_tag="ACHLAG_001938"
     CDS             9685..11946
                     /gene="metE"
                     /locus_tag="ACHLAG_001938"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFP0655179.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(11985..12800)
                     /gene="ysgA"
                     /locus_tag="ACHLAG_001939"
     CDS             complement(11985..12800)
                     /gene="ysgA"
                     /locus_tag="ACHLAG_001939"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFP0655180.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13062..13823
                     /gene="udp"
                     /locus_tag="ACHLAG_001940"
     CDS             13062..13823
                     /gene="udp"
                     /locus_tag="ACHLAG_001940"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFP0655181.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            13964..15391
                     /gene="rmuC"
                     /locus_tag="ACHLAG_001941"
     CDS             13964..15391
                     /gene="rmuC"
                     /locus_tag="ACHLAG_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFP0655182.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15486..16241
                     /gene="ubiE"
                     /locus_tag="ACHLAG_001942"
     CDS             15486..16241
                     /gene="ubiE"
                     /locus_tag="ACHLAG_001942"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFP0655183.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16255..16860
                     /gene="ubiJ"
                     /locus_tag="ACHLAG_001943"
     CDS             16255..16860
                     /gene="ubiJ"
                     /locus_tag="ACHLAG_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFP0655184.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            16857..18497
                     /gene="ubiB"
                     /locus_tag="ACHLAG_001944"
     CDS             16857..18497
                     /gene="ubiB"
                     /locus_tag="ACHLAG_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFP0655185.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18576..18845
                     /gene="tatA"
                     /locus_tag="ACHLAG_001945"
     CDS             18576..18845
                     /gene="tatA"
                     /locus_tag="ACHLAG_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFP0655186.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            18849..19364
                     /gene="tatB"
                     /locus_tag="ACHLAG_001946"
     CDS             18849..19364
                     /gene="tatB"
                     /locus_tag="ACHLAG_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFP0655187.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19367..20143
                     /gene="tatC"
                     /locus_tag="ACHLAG_001947"
     CDS             19367..20143
                     /gene="tatC"
                     /locus_tag="ACHLAG_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFP0655188.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20185..20967
                     /gene="tatD"
                     /locus_tag="ACHLAG_001948"
     CDS             20185..20967
                     /gene="tatD"
                     /locus_tag="ACHLAG_001948"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFP0655189.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(20964..21452)
                     /gene="rfaH"
                     /locus_tag="ACHLAG_001949"
     CDS             complement(20964..21452)
                     /gene="rfaH"
                     /locus_tag="ACHLAG_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFP0655190.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21619..23112
                     /gene="ubiD"
                     /locus_tag="ACHLAG_001950"
     CDS             21619..23112
                     /gene="ubiD"
                     /locus_tag="ACHLAG_001950"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFP0655191.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23158..23859
                     /gene="fre"
                     /locus_tag="ACHLAG_001951"
     CDS             23158..23859
                     /gene="fre"
                     /locus_tag="ACHLAG_001951"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFP0655192.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24051..25214)
                     /gene="fadA"
                     /locus_tag="ACHLAG_001952"
     CDS             complement(24051..25214)
                     /gene="fadA"
                     /locus_tag="ACHLAG_001952"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFP0655193.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYCPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25224..27413)
                     /gene="fadB"
                     /locus_tag="ACHLAG_001953"
     CDS             complement(25224..27413)
                     /gene="fadB"
                     /locus_tag="ACHLAG_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFP0655194.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPNTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSLLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDTIDALFDANRFGQKNGLGFWRYKEGSKGKPKKEEDAVVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEESIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27603..28934
                     /gene="pepQ"
                     /locus_tag="ACHLAG_001954"
     CDS             27603..28934
                     /gene="pepQ"
                     /locus_tag="ACHLAG_001954"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFP0655195.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERVLQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            28934..29548
                     /gene="yigZ"
                     /locus_tag="ACHLAG_001955"
     CDS             28934..29548
                     /gene="yigZ"
                     /locus_tag="ACHLAG_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFP0655196.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29587..31038
                     /gene="trkH"
                     /locus_tag="ACHLAG_001956"
     CDS             29587..31038
                     /gene="trkH"
                     /locus_tag="ACHLAG_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFP0655197.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31050..31595
                     /gene="hemG"
                     /locus_tag="ACHLAG_001957"
     CDS             31050..31595
                     /gene="hemG"
                     /locus_tag="ACHLAG_001957"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFP0655198.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7345 a         8101 c         8712 g         7596 t
ORIGIN      
        1 tcagtgggta ggctctttaa acggcctacc catgttaatt cttaactttt tctacgctgc
       61 gtataaatcg catccatcac aaaaattgcc agcgccaccc aaataaaggc gaaagtcacc
      121 atcttatcgg cacccggttt ttcaccataa aacgtcacag ccagcaggaa catcagcgtc
      181 gggccaatgt actggaaaaa gcctaacgtt gagagacgca agcgcgtggc agcggcggta
      241 aaacacaaca gcggtacggt agtgacaata ccggcggcga tcagcagtaa attcagcgac
      301 atcgggtttt gccccatatg gctggttgag ctgtcggcaa tagcaaacag gtaaattgcc
      361 gccacgggca gcagccacat ggtttcgatt aacatgccgg tttgcgcttc aacggcaatc
      421 ttcttgcgta ccagaccgta gaaggcaaaa ctaaatgcca gtcccagcgc gataataggt
      481 agcgaaccaa aagtccacag ctggactaac acgccacata tcgccagaat caccgccagc
      541 cattgcatcc ggcggaatcg ctcgccgagg aaaatcatcc ccagcacaat gttcaccagc
      601 gggttaataa agtaaccaag gctcgcttcc agcatatggt gattgttcac cgcccagata
      661 aacagtagcc agttgccacc aatcagcacg gcagagattg ccagcataaa aattttctgt
      721 ggcgtctgaa tcagcgtttt taaataggac cactggcggc aaatgctcat cagcaccacc
      781 ataaagaaaa acgaccagat cacgcgatgc gtcaggattt catcggcagg cacgtagtaa
      841 atcaacttga agtacgctgg cgctataccc caaataaaat aagcggcaag agcgagtaat
      901 acgccctgcc gcgtttgttt tgcatccatc gggaatactc atttttaatt ggtaacagca
      961 gtttacctgc ttttatgtct tcaacctacc atataggtgg cggtggcact ggcaatataa
     1021 agctgttctt cattgtgtaa ttcaacgcgg gcgacggcga ctttattgcc tgcacgcaac
     1081 aggctactgg tagcagtaaa acgctcgccc ctgcctgggc gcagataatc aacgcgaaga
     1141 tcaatggtcc ccatccgcga tagccgctgg cgtagttcat cttcactgat ggtttcgtgg
     1201 cgggttaagg tacttcccac gcacaccaga ccggcggcga catccagcgc cgacgcaatg
     1261 accccgccgt gcaaaatgct ttgcgcccag ttgcccacca tcattggctg atttttaaag
     1321 gccagctgtg cgaactcttt ttcgtaacgc tccagttcca tccccaatgc gcggttaaat
     1381 ggcatgtgat aaacaaacat ctcacccact aatttcaggg cttgttcagc ggtcagtacg
     1441 gcagacatat catccttaca cttcattggt taatgaaatg ttgattttat gcttctttgt
     1501 tgttggtttc tactttagga agggataact aacggctatg gagttaagta tgtaaaatag
     1561 cccgcagaaa aatattcacc ttatcaataa ttcgttacgg agaacacgac cgatgcggac
     1621 tctgcagggc tggttgttgc cggtgtttat gttgcctatg gcagtatatg cgcaagaggc
     1681 aacggtgaaa gaggtgcatg acgcgccagc ggtgcgtggc agtattatcg ccaatatgct
     1741 gcaggagcat gacaatccgt tcacgctcta tccttatgac accaactacc tcatttacac
     1801 ccaaaccagc gatctgaata aagaagcgat tgccagttac gactgggcgg aaaatgcgcg
     1861 taaggatgaa gtaaagtttc agttgagcct ggcatttccg ctgtggcgtg ggattttagg
     1921 cccgaactcg gtgttgggtg cgtcttatac gcaaaaatcc tggtggcaac tgtccaatag
     1981 cgaagagtct tcaccgtttc gtgaaaccaa ctacgaaccg caattgttcc tcggttttgc
     2041 caccgattac cgttttgcag gttggacgct gcgcgatgtg gagatggggt ataaccacga
     2101 ctctaacggg cgttccgacc cgacctcccg cagctggaac cgcctttata ctcgcctgat
     2161 ggcagaaaac ggtaactggc tggtagaagt gaagccgtgg tatgtggtgg gtaatactga
     2221 cgataacccg gatatcacca aatatatggg ttactaccag cttaaaatcg gctatcacct
     2281 cggtgatgcg gtgctcagtg cgaaaggaca gtacaactgg aacaccggct acggcggcgc
     2341 ggagttaggc ttaagttacc cgatcaccaa acatgtgcgc ctttatactc aggtttacag
     2401 cggctatggc gaatcgctca tcgactataa cttcaaccag acccgtgtcg gtgtgggggt
     2461 tatgctaaac gatttgtttt gatgaacggt tgagtggttg gcaaatctgg aatccagcat
     2521 ccaggattac cctctcagag actaaaagca ttgcagtttc tcgcgcaggc gctgaaaata
     2581 gcgcctgttt ttatttcagg caatcggggt gaatgtggcg caggcggaag tgttgaatct
     2641 ggagtccgga gctaaacagg ttttacaaga aacctttggc taccaacagt ttcgccccgg
     2701 ccaggaagaa attatcgaca ctgtgctttc cggtcgcgat tgcctggtcg tcatgcccac
     2761 cggtggcgga aaatcccttt gctatcaaat tcctgcctta ttgctaaacg gccttaccgt
     2821 ggttgtttca ccgctgattt cgttgatgaa agatcaggtg gatcaactgc aagccaacgg
     2881 cgtggcggcg gcgtgcctta actcgacgca aacccgcgaa cagcaacttg aagtgatgac
     2941 aggctgccgc accgggcaaa ttcgtctgct ttatatcgcc ccggaacgcc tgatgctgga
     3001 taactttctt gagcatctgg cgcactggaa tccggtgtta ttagccgttg atgaagcgca
     3061 ctgtatctcc caatggggcc acgatttccg cccggaatat gccgcgctcg gtcagttgcg
     3121 ccagcggttc ccgacgctgc cgtttatggc gctgaccgcc acagccgacg acaccacgcg
     3181 ccaggatatc gtgcgcctgc tggggctgaa cgatccgctg attcaaatca gcagttttga
     3241 ccgtccgaat attcgctaca tgctgatgga gaagttcaaa ccgctcgatc agttgatgcg
     3301 ctacgtgcag gaacagcgcg gtaagtccgg cattatctac tgcaacagcc gggcgaaagt
     3361 agaagacacc gctgcgcgcc tgcaaagcaa gggtattagc gcggcggcct atcatgccgg
     3421 gctggaaaat aatgtccgcg ccgacgtgca ggagaaattc cagcgcgatg acctgcaaat
     3481 tgtggtggcg acggtggcgt tcggcatggg catcaataaa ccaaacgttc gcttcgtggt
     3541 ccactttgat attccgcgca atatcgaatc ctattatcag gaaaccggtc gcgccgggcg
     3601 tgatggcctg cccgcggaag cgatgctgtt ttacgatccg gccgatatgg cgtggcttcg
     3661 ccgttgtctg gaagagaagc cgcaggggca gttgcaggat atcgaacgtc ataaactcaa
     3721 tgcgatgggc gcttttgccg aagcgcaaac ttgccgtcgt ctggtactgc tgaactattt
     3781 tggcgaaggg cgtcaggagc cgtgcgggaa ctgcgatatc tgcctcgatc cgccgaaaca
     3841 gtacgacggt tcaaccgatg ctcagattgc cctttccacc attggtcgtg tgaatcagcg
     3901 ttttggtatg ggctatgtgg tggaagtgat tcgcggcgct aataaccagc gtatccgcga
     3961 ctatggtcat gacaaactaa aagtctacgg tatgggccgt gataaaagtc atgaacattg
     4021 ggtgagcgtg atccgccagc tgattcacct cggtctggtg acgcaaaata ttgcccagca
     4081 ttctgcccta caactgacag aggctgcgcg tccggtgctg cgcggcgaat cctctttgca
     4141 acttgccgtg ccgcgtatcg tggcgctcaa accgaaagcg atgcagaaat cgttcggcgg
     4201 caactatgat cgcaaactgt tcgccaaatt acgcaaactg cgtaaatcga tagccgatga
     4261 aagtaatgtc ccgccgtacg tggtgtttaa cgacgcaacc ttgattgaga tggctgaaca
     4321 gatgccgatc accgccagcg aaatgctcag cgttaacggc gttgggatgc gcaagctgga
     4381 acgctttggc aaaccgttta tggcgctgat tcgtgcgcat gttgatggcg atgacgaaga
     4441 gtagtcagca gcataaaaaa gtgccagtat gaagactccg taaacgtttc ccccgcgagt
     4501 caaatgtatg ttgatgttat ttctcaccgt cgccatggtg cacattgtgg cgcttatgag
     4561 ccccggtccc gatttctttt ttgtctctca gaccgctgtc agtcgttccc gtaaagaagc
     4621 gatgatgggc gtgctgggca ttacctgcgg cgtaatggtt tgggctggga ttgcgctgct
     4681 tggcctgcat ttgattatcg aaaaaatggc ctggctgcat acgctgatta tggtgggcgg
     4741 tggcctgtat ctctgctgga tgggttacca gatgctacgc ggtgcactga aaaaagaggc
     4801 ggtttctgca cctgcgccac aggtcgagct ggcgaaaagt gggcgcagtt tcctgaaagg
     4861 tttactgacc aatctcgcta atccgaaagc gattatctac tttggctcgg tgttctcatt
     4921 gtttgtcggt gataacgttg gcactaccgc gcgctggggc atttttgcgc tgatcattgt
     4981 cgaaacgctg gcgtggttta ccgtcgttgc cagcctgttt gccctgccgc aaatgcgccg
     5041 tggttatcaa cgtctggcga agtggattga tggttttgcc ggggcgttat ttgccggatt
     5101 tggcattcat ttgattattt cgcggtgatg ccagacgcgt cttcagagta agtcggataa
     5161 ggcgtttacg ccgcatccga cattattttt cacgcatgcc tcgccgatgc taacagcgct
     5221 cccaccagca taaacaacga gccgaaaatc ttattcagcg ccttcatctg ctttggtcct
     5281 ttaatccata gagcaatccg ttgagcaagg gtggcgtaac cgatcatcac aataatatcg
     5341 accacaatag tggtgacgcc gagcacgata tactgcatca gttgcggctg ttgcggcatg
     5401 atgaattgcg gaaatagcgc cgccagaaac acaatacttt tgggattggt gagattcaca
     5461 aaaactgcgc gctggaacaa atgtcgacgc gattgagtag aggccagcga tttaaggtca
     5521 attgcaccag cggcgcgcca ctgctggatt cccagccaaa tcaagtaagc cgcgcctgcc
     5581 cacttcaaca cttcaaacgc aatcactgag cgggaaaata gcgtccccaa ccccacgcca
     5641 accaacacaa tatgaatcgc cagtccggtc tgaagcccag caatagacgc caccgcgccg
     5701 cgataaccgt ggttgagcga ggtggtcata gtgttgattg caccagagcc tggcgacagg
     5761 cttaaaatga tcgatgtcag caggtaggca aaccaccatt ctaaggtcat gatgaactcc
     5821 cggtgtgtct atttttgtgc cacaatacgc tactgtcgca gcgttgtgtc aggcacgcta
     5881 aaaaaaacga ttttacgtgg tttaagaggc agattacccg atgtttcagc agcaaaaaga
     5941 ctgggaaaca agagaaaacg cgtttgctgc ttttaccatg ggaccgctga ctgatttctg
     6001 gcgtcagcgt gatgaagcag agtttactgg tgtggatgac attccggtgc gctttgtccg
     6061 ttttcgcgca cagcaccatg accgggtggt agtcatctgc ccggggcgta ttgagagcta
     6121 cgtaaaatat gcggaactgg cctatgacct gttccatttg gggtttgatg tcttaatcat
     6181 cgaccatcgc gggcagggac gttccggtcg cctgttagcc gatccgcatc tcgggcatgt
     6241 taatcgcttt aatgattatg ttgatgatct ggcggcattc tggcagcagg aggttcagcc
     6301 cggtccgtgg cgtaaacgct atatactggc acattcgatg ggcggtgcga tctccacatt
     6361 atttctgcaa cgccatccag gtgtatgtga cgccattgcg ctaactgcgc caatgtttgg
     6421 gatcgtgatt cgtatgccgt catttatggc acggcagatc ctcaactggg ccgaagcgca
     6481 tccacgtttc cgtgatggct atgcaatagg caccgggcgc tggcgcgcgt tgccgtttgc
     6541 tatcaacgta ctgacccaca gcagacagcg atatcgacgt aacttacgct tctatgctga
     6601 tgacccaacg attcgcgtcg gtgggccgac ctaccattgg gtacgcgaaa gtattctggc
     6661 tggcgaacag gtgttagccg gtgcgggtga tgatgccacg ccaacgcttc tcttacaagc
     6721 tgaagaggaa cgcgtggtgg ataaccgcat gcatgaccgt ttttgtgaac tccgcaccgc
     6781 cgcgggccat cctgtcgaag gaggacggcc gttggtaatt aaaggtgctt accatgagat
     6841 cctttttgaa aaggacgcaa tgcgctcagt cgcgctccac gccatcgttg attttttcaa
     6901 caggcataac tcacccagcg gaaaccgctc tacagaggtt taaatttctt atgtaccagg
     6961 ttgttgcgtc tgatttagat ggcacgttac tttctcccga ccatacgtta tccccttacg
     7021 ccaaagaaac tctgaagctg ctcaccgcgc gcggcatcaa ctttgtgttt gcgaccggtc
     7081 gtcaccacgt tgatgtgggg caaattcgcg ataatctgga gattaagtct tacatgatta
     7141 cctccaatgg tgcgcgcgtt cacgatctgg atggtaatct gatttttgct cataacctgg
     7201 atcgcgacat tgccagcgat ctgtttggcg tagtcaacga caatccggac atcattacta
     7261 acgtttatcg cgacgacgaa tggtttatga atcgccatcg cccggaagag atgcgctttt
     7321 ttaaagaagc ggtgttcaaa tatgcgctgt atgagcctgg attactggag ccggaaggcg
     7381 tcagcaaagt gtttttcacc tgcgattccc atgaacaact gctgccgctg gagcaggcga
     7441 ttaacgctcg ctggggcgat cgcgtcaacg tcagtttctc taccttaacc tgtctggaag
     7501 tgatggcggg cggcgtgtcg aaaggccatg cgctggaagc agtggcgaag aaactgggct
     7561 acagcctgaa ggattgtatt gcgtttggtg acgggatgaa cgacgccgaa atgctgtcga
     7621 tggcggggaa aggctgcatt atgggcagtg cgcaccagcg tctgaaagac cttcatcccg
     7681 agctggaagt gattggtact aatgccgacg acgcggtgcc gcattatctg cgtaaactct
     7741 atttatcgta atcgttcttt atttggtcag ttgtcaacct gatacttcgc tacaatggat
     7801 actcgttaat caaagagttt tccattgtgg cgctacttat catcaccacg attctgtggg
     7861 ccttctcctt tagcttttat ggcgagtacc ttgcggggca cgtcgatagc tattttgcgg
     7921 tgctggtgcg cgttggcctg gcggcactcg tttttctgcc gtttctgcgt acccgtggca
     7981 atagcctgaa aacggtcggc ctgtatatgt tggtgggcgc gatgcagctc ggcgtgatgt
     8041 atatgctgag tttccgcgcg tatctctacc tgacggtctc cgaactgctg ctgttcaccg
     8101 tactgacgcc gctctacatc acgctgattt atgacatcat gagtaagcgc cgtctgcgct
     8161 ggggctatgc ttttagcgcc ttgctggcgg tgattggtgc cgggattatt cgctatgatc
     8221 aggtcaccga ccatttctgg actggcttgt tgctggtgca actctccaat atcacttttg
     8281 ccatcggcat ggtgggttac aaacgcctga tggaaactcg cccgatgccg cagcataacg
     8341 cctttgcgtg gttctatctt ggcgcgttcc tggtggcggt aattgcatgg ttcttgctgg
     8401 gaaatgcgca gaaaatgccg caaaccacgt tgcaatgggg cattctggtg tttcttggcg
     8461 tggtggcttc cgggattggc tactttatgt ggaactacgg cgcgacgcag gtggacgccg
     8521 gaacgctggg cattatgaat aatatgcacg ttccggcagg gctgctggtt aacctggcta
     8581 tctggcacca acagctgcac tggccaacgt ttattacagg cgcgctggtg atcctggcct
     8641 cactgtgggt gcatcgtaag tgggtcgctc cgcgctcttc acaaacggca gatgatcgca
     8701 ggcgtgattg cgcgctgagc gaataaacgc ttccgtaact ggctgacgct gctcgccatc
     8761 gcgcacggcg gcgtacagtc ggctccacaa gccttcgccc agggttttgg tcaccaccag
     8821 accctggcgc tcaaaactct ctactaccca atgcggtagc gcggcaatac ccatccgcgc
     8881 ggcaaccatc tgaatcaaca ataaggtgtt atcgacgctt ttcagtgacg ggctgacgcc
     8941 tgccggctga agaaaatgcc gccagacatc cagtcgacta cgctgcaccg gataaattaa
     9001 tagcgtctcg ctggcgagat cttccggtgt aatacgcgtt tttgccgcca gtggatggtc
     9061 aggtgctaac accaggcgta cttcataatc gaacatcggc gaataatgca ggccactgcg
     9121 cggcaggata tcggaggtca tcaccagatc cagctcgccc tgttgcaacg cgggctgcgg
     9181 gtcaaacgtc acgcccgatt taaaatccat ctctacctgc ggccagttct tatggaaatt
     9241 ttctaacgcg ggtgtcagcc actgaataca gctatggcac tcaatggcaa tgcgcagacg
     9301 cgtctgctgc ggttcattgc aggcttgtag tgcctggctg atttgcggca gcacctggtt
     9361 tgccagttgc aacaggattt ctccctgcgg tgtaaagcgt agcggctggc tcttacgcac
     9421 aaatagccgg aaaccaaggc gttgttccag atcgctaaac tggtgagaca gggcggattg
     9481 cgtctgatgc aacgtcgccg cagcggctgc gagcgagccg cagttccgca acgcttgtag
     9541 cgttttcagg tgttttactt cgatcatgaa agtccttcac ttcggcatga ataatttgcg
     9601 cttgaggaat atacagtaac cgccaattat ggatgtgtaa acatctggac ggctaaatac
     9661 taaaaattcc ataaggggca tataatgaca attcttaatc acaccctcgg tttccctcgc
     9721 gttggcctgc gtcgcgagct gaaaaaagcg caagaaagtt attgggcggg gaactccacg
     9781 cgtgaagaac tgctgacagt agggcgtgaa ctgcgtgctc gtcactggga tcaacaaaag
     9841 caagcgggta tcgacctgct gccggtgggc gattttgcct ggtacgatca tgtactgacc
     9901 accagtttgc tgctgggtaa tgttccgcca cgtcatcaga acaaagacgg ttcggtagat
     9961 atcgacaccc tgttccgtat tggtcgtgga cgtgcgccga ctggcgaacc tgcggcggca
    10021 gcggaaatga ccaaatggtt taacaccaac tatcactaca tggtgccgga gttcgttaaa
    10081 ggccaacagt tcaaactgac ctggacgcag ctgctggagg aagtggacga ggcgctggcg
    10141 ctgggccaca acgttaagcc tgtgctgctg gggccggtta cttacctgtg gctggggaaa
    10201 gtgaaaggtg aacagtttga ccgccttagc ctgctgaacg acattctgcc ggtttatcag
    10261 caagtgctgg cagaactggc gaaacgcggc atcgagtggg tacagattga tgaacccgcg
    10321 ctggtactgg aactaccaca ggcgtggctg gacgcataca aacccgctta cgacgcgctc
    10381 cagggacagg tgaaactgct gctgaccacc tattttgaag gcgtaacgcc aaatctcgac
    10441 acgattactg cgctgcctgt tcagggtctg catgttgacc tcgtacatgg taaagatgac
    10501 gttgctgaac tgcacaagcg cctgccttct gactggttgc tgtctgcggg tctgatcaat
    10561 ggtcgtaacg tctggcgcgc cgatcttacc gagaaatatg cgcaaattaa ggacattgtc
    10621 ggcaaacgtg atttgtgggt ggcatcttcc tgctcgttgc tgcacagccc catcgacctg
    10681 agcgtggaaa cgcgtcttga tgcagaagtg aaaagctggt ttgccttcgc cctacaaaaa
    10741 tgccatgaac tggcactgct gcgcgatgcg ctgaacagtg gtgacacggc agctctggca
    10801 gagtggagcg ccccaattca ggcgcgtcgt cactctactc gtgtacataa tccggcggta
    10861 gaaaagcgtc tggcggcgat caccgcccag gacagccagc gtgcgaatgt ctatgaagtg
    10921 cgtgctgaag cccagcgtgc gcgttttaaa ctgccagcgt ggccgaccac cacgattggt
    10981 tccttcccgc aaaccacgga aattcgtacc ctgcgtctgg atttcaaaaa gggcaatctc
    11041 gacgccaaca actaccgcac gggcattgcg gaacatatca agcaggccat tgttgagcag
    11101 gaacgtttgg gactggatgt gctggtacat ggcgaggccg agcgtaatga catggtggaa
    11161 tactttggcg agcacctcga cggatttgtc tttacgcaaa acggttgggt acagagctac
    11221 ggttcccgct gcgtgaagcc accgattgtt attggtgacg tgagccgacc ggcaccgatt
    11281 accgtggagt gggcgaaata tgcgcagtcg ctgaccgaca aaccggtgaa agggatgctg
    11341 actggtccgg tgacgattct ctgctggtcg ttcccgcgtg aagatgtgag ccgtgaaacc
    11401 atcgccaaac agattgcgct ggcgctgcgt gatgaagtgg cggacctgga agccgctgga
    11461 attggcatca tccagattga tgaaccggcg ctgcgcgaag gtttaccgct gcgtcgtagc
    11521 gactgggacg cctatctcca gtggggcgta gaggccttcc gtatcaacgc cgccgtggcg
    11581 aaagatgaca cacaaatcca cactcacatg tgttattgcg agttcaacga catcatggat
    11641 tccattgcgg cgctggatgc ggatgtgatt accatcgaaa cttcgcgttc tgacatggag
    11701 ttgctggagt cgttcgaaga gtttgattat cccaatgaaa tcggtccagg cgtctatgat
    11761 attcactcgc caaacgtacc gagcgtggaa tggattgaag ccctgctgaa gaaagcggca
    11821 aaacgcattc cggcagagcg cctgtgggtt aacccggact gtggcctgaa aacgcgcggc
    11881 tggccagaaa cccgcgcggc actggcgaac atggtgcagg cggcgcagaa cttgcgtcgg
    11941 gggtaaatcc aaaccgggtg gtaataccac ccggtctttt ctcattacag cgacttcttc
    12001 ccaccatact gcttaaacca ttccagcata cgctgccagc catctttcgc agattcggca
    12061 tgatagctcg ggcgataatc agcgttgaat gcatgcccgg catccgggta cacgataatc
    12121 tctgctttcg cattagcagc ccgcagcgcc tggcgcatgg tttcaacgct ctcctgcgga
    12181 atgctgttat cctgaccacc atataagccg agaaccggcg cgttaagatc ggttgcgata
    12241 tcaacaggtt gtttcggtga attcagcgac ttgtcgcccg tcagtttgcc gtaccacgcc
    12301 actgcggctt ttagctgtgg attatgcgcg gcatacagcc aggtgatacg tccaccccag
    12361 cagaatccgg tgatcattaa acgatgaaca tcgccgccgt tgcgggaagc ccaactggcg
    12421 acatgatcga gatcggccag cacctgcgag tcaggcactt ttgctaccag accgctaagc
    12481 aacgtgggga tatcggcaaa atcattcgga tcgccttcgc ggaagtaaag ttcaggtgcg
    12541 atagccagat acccctccag cgccagacgg cgacaaatgt cgcggatatg ttcatgcacg
    12601 ccaaaaattt cctgcactac aatgaccact ggcagtgggc catcgctttg ctttggtctg
    12661 gcatggtaag caggcatgtt atccccttgt gaagggatag aggtgaagcc cgccacaatc
    12721 gcgtcgtccg gggtctgaac gatggtcgaa gcgagaggcg atgcagcagg tgcaaatcca
    12781 gattgttgtg ttgttgccat ggtattctcc gtacccttat aaaaatgttg cgcaatgtta
    12841 actatagtca gcatgcaaca aatcacattg cctgaatcgg ctcatctttt atgcagtcct
    12901 gcagaatgaa gagtgattta tgtgatttac atcacttttg atgggtaaat ttatgcaacg
    12961 cgtttgcgtc atggtgatga gtatcacgaa aaaatgttaa acccttcggt aaagtgtctt
    13021 tttgcttctt ctgactaaac cgattcacag aggagttgta tatgtccaag tctgatgttt
    13081 ttcatctcgg cctcactaaa aacgatttac aaggggctac gcttgccatc gtccctggcg
    13141 acccggatcg tgtggaaaag atcgccgcgc tgatggataa gccggttaag ctggcatctc
    13201 accgcgaatt cactacctgg cgtgcagagc tggatggtaa acctgttatc gtctgttcta
    13261 ccggtatcgg cggcccgtct acctctattg ctgttgaaga gctggcacag ctgggcattc
    13321 gcaccttcct gcgtatcggt acaacgggcg ctattcagcc gcatattaat gtgggtgatg
    13381 tcctggttac cacggcgtct gtccgtctgg atggcgcgag cctgcacttc gcaccgctgg
    13441 aattcccggc tgtcgctgat ttcgaatgta cgactgcgct ggttgaagct gcgaaatcca
    13501 ttggcgcgac aactcacgtt ggcgtgacag cttcttctga taccttctac ccaggtcagg
    13561 aacgttacga tacttactct ggtcgcgtag ttcgtcactt taaaggttct atggaagagt
    13621 ggcaggcgat gggcgtaatg aactatgaaa tggaatctgc aaccctgctg accatgtgtg
    13681 caagtcaggg cctgcgtgcc ggtatggtag cgggtgttat cgttaaccgc acccagcaag
    13741 agatcccgaa tgctgagacg atgaaacaaa ccgaaagcca tgcggtgaaa atcgtggtgg
    13801 aagcggcgcg tcgtctgctg taattctctt ctcctgtctg aaggccgacg cgttcggcct
    13861 tttgtatttt tgcgtagcgc ctcgcaggaa atgcctttcc aactggacgt ttgtacagca
    13921 caattctatt ttgtgcgggt aagttgttgc gtcaggaggc gttgtggatt tctcaatcat
    13981 ggtttacgca gttattgcgt tggtgggtgt ggcaattggc tggctgtttg ccagttatca
    14041 acatgcgcag caaaaagccg agcaattagc tgaacgtgaa gagatggtcg cggagttaag
    14101 cgcggcaaaa caacaaatta cccaaagcga gcactggcgt gcagagtgcg agttactcaa
    14161 taacgaagtg cgcagcctgc aaagtattaa cacctctctg gaggccgatc tgcgtgaagt
    14221 aaccacgcgg atggaagccg cacagcaaca tgctgacgat aaaattcgcc agatgattaa
    14281 cagcgagcag cgcctcagtg agcagtttga aaacctcgcc aaccgtattt ttgagcacag
    14341 caatcgccgg gttgatgagc aaaaccgtca gagtctgaac agcctgttgt cgccgctacg
    14401 tgaacaactg gacggttttc gccgtcaggt tcaggacagc ttcggtaaag aagcacaaga
    14461 acgccatacc ctgacccacg aaattcgcaa tctccagcaa ctcaacgcgc aaatggccca
    14521 ggaagcgatc aacctgacgc gcgcgctgaa aggcgacaat aaaacccagg gcaactgggg
    14581 cgaggtagta ttgacgcggg tgctggaggc ttccggtctg cgtgaagggt atgaatatga
    14641 aacccaggtc agcatcgaaa atgacgcccg ctcgcggatg caaccggatg tcatcgtgcg
    14701 gctgccgcag ggaaaagatg tggtgatcga cgccaaaatg acgctggtcg cctatgaacg
    14761 ctatttcaac gccgaagatg actacacccg cgaaagcgcg ctacaggaac atatcgcgtc
    14821 ggtgcgtaac catatccgtt tgctgggacg caaagattat caacagctgc cggggctgcg
    14881 aactctggat tacgtgctga tgtttattcc cgttgaaccc gcttttttac tggcgcttga
    14941 ccgccagccg gagctgatca ccgaagcgtt gaaaaacaat atcatgctgg ttagcccgac
    15001 tacgctgctg gtggcgttgc gtactattgc caacctgtgg cgttatgagc atcaaagccg
    15061 caacgcccag caaatcgccg atcgtgccag caagctgtac gacaagatgc gtttgttcat
    15121 cgatgacatg tccgcgattg gtcaaagtct cgacaaagcg caggataact accgccaggc
    15181 aatgaaaaaa ctctcttcag gtcgcggaaa tgtgctggcg caggcagaag cgtttcgcgg
    15241 tttaggagta gaaattaaac gcgagattaa tccggatttg gctgaacagg cggtgagcca
    15301 ggatgaagag tatcgacttc ggtcggttcc ggagcagccg aatgatgaag cttatcaacg
    15361 cgatgatgaa tataatcagc agtcgcgcta gcccattagg ggtagttaag ccgggtagaa
    15421 atctagggca tcgacgccca atctgttaca cttctggaac aattttttga tgagcaggca
    15481 ttgagatggt ggataagtca caagaaacga cgcactttgg ttttcagacc gtcgcgaagg
    15541 aacaaaaagc ggatatggtc gcccacgttt tccattccgt ggcatcaaaa tacgatgtca
    15601 tgaatgattt gatgtcattt ggtattcatc gtttgtggaa gcgattcacg attgattgca
    15661 gcggcgtacg ccgtgggcag accgtgctgg atctggctgg tggcaccggc gacctgacag
    15721 cgaaattctc ccgcctggtc ggagaaactg gcaaagtggt ccttgctgat atcaatgaat
    15781 ccatgctcaa aatgggacgc gagaagctgc gtaatatcgg tgtgattggc aacgttgagt
    15841 atgttcaggc gaacgctgag gcgctgccgt tcccggataa cacctttgat tgcatcacca
    15901 tttcgtttgg tctgcgtaac gtcaccgaca aagataaagc actgcgttca atgtatcgcg
    15961 tgctgaaacc cggcggccgc ctgctggtgc ttgagttctc gaagccaatt atcgagccgc
    16021 tgagcaaagc ctatgatgca tactccttcc atgtgctgcc gcgtattggc tcactggtcg
    16081 cgaacgacgc cgacagctac cgttatctgg cagaatccat ccgtatgcat cccgatcagg
    16141 ataccctgaa agccatgatg caggatgccg gattcgaaag tgtcgactac tacaatctga
    16201 cggcaggggt tgtggcgctg catcgtggtt ataagttctg acaggagacc ggaaatgcct
    16261 tttaaacctt tagtgacggc aggaattgaa agtctgctca acaccttcct gtatcgctca
    16321 cccgcgctga aaacagcccg ctcgcgtctg ctgggtaaag tattgcgcgt ggaggtaaaa
    16381 ggcttttcga cgtcattgat tctggtgttc agcgaacgcc aggttgatgt actgggcgaa
    16441 tgggcaggcg atgctgactg caccgttatc gcctacgcca gtgtgttgcc gaaacttcgc
    16501 gatcgccagc agcttaccgc actgattcgc agtggtgagc tggaagtgca gggcgatatt
    16561 caggtggtgc aaaacttcgt tgcgctggca gatctggcag agttcgaccc tgcggaactg
    16621 ctggcccctt ataccggtga tatcgccgct gaaggaatca gcaaagccat gcgcggaggc
    16681 gcaaagttcc tgcatcacgg cattaagcgc cagcaacgtt atgtggcgga agccattact
    16741 gaagagtggc gtatggcacc cggtccgctt gaagtggcct ggtttgcgga agagacggct
    16801 gccgtcgagc gtgctgttga tgccctgacc aaacggctgg aaaaactgga ggctaaatga
    16861 cgccaggtga agtacggcgc ctatatttca tcattcgcac ttttttaagc tacggacttg
    16921 atgaactgat ccccaaaatg cgtatcaccc tgccgctacg gctatggcga tactcattat
    16981 tctggatgcc aaatcggcat aaagacaaac ttttaggtga gcgactacga ctggccctgc
    17041 aagaactggg gccggtttgg atcaagttcg ggcaaatgtt atcaacccgc cgcgatcttt
    17101 ttccaccgca tattgccgat cagctggcgt tattgcagga caaagttgct ccgtttgatg
    17161 gcaagctggc gaagcagcag attgaagctg caatgggcgg cttgccggta gaagcgtggt
    17221 ttgacgattt tgaaatcaag ccgctggctt ctgcttctat cgcccaggtt cataccgcgc
    17281 gattgaaatc gaatggtaaa gaggtggtga ttaaagtcat ccgcccggat attttgccgg
    17341 tcattaaagc ggatctgaaa cttatctacc gtctggctcg ctgggtgccg cgtttgctgc
    17401 cggatggtcg ccgtctgcgc ccaaccgaag tggtgcgcga gtacgaaaag accttgattg
    17461 atgaactgaa tttgctgcgg gaatctgcca acgccattca gcttcggcgc aattttgaag
    17521 acagcccgat gctctacatc ccggaagttt accctgacta ttgtagtgaa gggatgatgg
    17581 tgatggagcg catttacggc attccggtgt ctgatgttgc ggcgctggag aaaaacggca
    17641 ctaacatgaa attgctggcg gaacgcggcg tgcaggtgtt cttcactcag gtctttcgcg
    17701 acagcttttt ccatgccgat atgcaccctg gcaacatctt cgtaagctat gaacacccgg
    17761 aaaacccgaa atatatcggc attgattgcg ggattgttgg ctcgctaaac aaagaagata
    17821 aacgctatct ggcagaaaac tttatcgcct tctttaatcg cgactatcgc aaagtggcag
    17881 agctacacgt cgattctggc tgggtgccac cagataccaa cgttgaagag ttcgaatttg
    17941 ccattcgtac ggtctgtgaa cctatctttg agaaaccgct ggccgaaatt tcgtttggac
    18001 atgtactgtt aaatctgttt aatacggcgc gtcgcttcaa tatggaagtg cagccgcaac
    18061 tggtgttact ccagaaaacc ctgctctatg tcgaaggggt aggacgccag ctttatccgc
    18121 agctcgattt atggaaaaca gcgaagcctt tcctggagtc gtggattaaa gatcaggtcg
    18181 gtattcctgc gctggtgaga gcatttaaag aaaaagcgcc gttctgggtc gaaaaaatgc
    18241 cagaactgcc tgaattggtt tacgacagtt tgcgccaggg caagtattta cagcacagtg
    18301 ttgataagat tgcccgcgag cttcagtcaa atcatgtacg tcagggacaa tcgcgttatt
    18361 ttctcggaat tggcgctacg ttagtattaa gtggcacatt cttgttggtc agccgacctg
    18421 agtgggggct gatgcccggc tggttaatgg caggtggtct gatcgcctgg tttgtcggtt
    18481 ggcgcaaaac acgctgattt tttcatcgct caaggcgggc cgtgtaacgt ataatgcggc
    18541 tttgtttaat catcatctac cacagaggaa catgtatggg tggtatcagt atttggcagt
    18601 tattgattat tgccgtcatc gttgtactgc tttttggcac caaaaagctc ggctccatcg
    18661 gttccgatct tggtgcgtcg atcaaaggct ttaaaaaagc aatgagcgat gatgaaccaa
    18721 agcaggataa aaccagtcag gatgctgatt ttactgcgaa aactatcgcc gataagcagg
    18781 cggatacgaa tcaggaacag gctaaaacag aagacgcgaa gcgccacgat aaagagcagg
    18841 tgtaatccgt gtttgatatc ggttttagcg aactgctatt ggtgttcatc atcggcctcg
    18901 tcgttctggg gccgcaacga ctgcctgtgg cggtaaaaac ggtagcgggc tggattcgcg
    18961 cgttgcgttc actggcgaca acggtgcaga acgaactgac ccaggagtta aaactccagg
    19021 agtttcagga cagtctgaaa aaggttgaaa aggcgagcct cactaacctg acgcccgaac
    19081 tgaaagcgtc gatggatgaa ctacgccagg ccgcggagtc gatgaagcgt tcctacgttg
    19141 caaacgatcc tgaaaaggcg agcgatgaag cgcacaccat ccataacccg gtggtgaaag
    19201 ataatgaagc tgcgcatgag ggcgtaacgc ctgccgctgc acaaacgcag gccagttcgc
    19261 cggaacagaa gccagaaacc acgccagagc cggtggtaaa acctgctgcg gacgctgaac
    19321 cgaaaaccgc tgcaccttcc ccttcgtcga gtgataaacc gtaaacatgt ctgtagaaga
    19381 tactcaaccg cttatcacgc atctgattga gctgcgtaag cgtctgctga actgcattat
    19441 cgcggtgatc gtgatattcc tgtgtctggt ctatttcgcc aatgatatct atcacctggt
    19501 atccgcgcca ttgatcaagc agttgccgca aggttcaacg atgatcgcca ccgacgtggc
    19561 ctcgccgttc tttacgccga tcaagctgac ctttatggtg tcgctgattc tgtcagcgcc
    19621 ggtgattctc tatcaggtgt gggcatttat cgccccagcg ctgtataagc atgaacgtcg
    19681 cctggtggtg ccgctgctgg tttccagctc tctgctgttt tatatcggta tggcattcgc
    19741 ctactttgtg gtctttccgc tggcatttgg cttccttgcc aataccgcgc cggaaggggt
    19801 gcaggtatcc accgacatcg ccagctattt aagcttcgtt atggcgctgt ttatggcgtt
    19861 tggtgtctcc tttgaagtgc cggtggcaat tgtgctgctg tgctggatgg ggattacctc
    19921 gccagaagac ttacgcaaaa aacgcccgta tgtgctggtt ggtgcattcg ttgtcgggat
    19981 gttgctgacg ccgccggatg tcttctcgca aacgctattg gcgatcccga tgtactgtct
    20041 gtttgaaatc ggtgtcttct tctcacgctt ttacgttggt aaagggcgaa atcgggaaga
    20101 ggaaaacgac gctgaagcag aaagcgaaaa aactgaagaa taaattcaac cgcccgtcag
    20161 ggcggttgtc atatggagta caggatgttt gatatcggcg ttaatttgac cagttcgcaa
    20221 tttgcgaaag accgtgatga tgttgtagcg cgcgcttttg acgcgggagt taatgggcta
    20281 ctcatcaccg gcactaacct gcgtgaaagc cagcaggcgc aaaagctggc gcgtcagtat
    20341 tcgtcctgtt ggtcaacggc gggcgtacat cctcacgaca gcagccagtg gcaagctgcg
    20401 actgaagaag cgattattga gctggccgcg cagccagaag tggtggcgat tggtgaatgt
    20461 ggtctcgact ttaaccgcaa cttttcgacg ccggaagagc aggaacgcgc ttttgttgct
    20521 cagctacgca ttgccgcaga attaaacatg ccggtattta tgcactgtcg cgatgcccac
    20581 gagcggttta tgacattgct ggagccgtgg ctggataaac tgcctggtgc ggttcttcat
    20641 tgctttaccg gcacacgcga agagatgcag gcgtgcgtgg cgcgtggaat ttatatcggc
    20701 attaccggtt gggtttgcga tgaacgacgc gggctggagc tgcgggaatt gttgccgttg
    20761 attccggcgg agaaattgct gatcgaaact gatgcgccgt atctgctccc gcgcgatctc
    20821 acgccaaagc catcatcccg gcgcaacgag ccagcccatc tgccccatat tttgcaacgt
    20881 attgcgcact ggcgtggaga agatgccgca tggctggctg ccaccacgga tgccaatgtc
    20941 aaaacactgt ttgggattgc gttttagagt ttgcggaact cggtgttctt cacactgtgc
    21001 ttaatctctt tattaataag attaagcaat agcatggagc gagcctcacc atcgggttcg
    21061 gtgaaaatgg cctgaaagcc ttcgaacgcg ccttcggtaa taatcacctt atcacccgga
    21121 taaggggttg ccggatcgac aatgtctttc ggtttatata ccgatagctg atgaatcacc
    21181 gccgatggga ctatcgctgg cgacgcgcca aagcgcacga agtggctgac accgcgggtc
    21241 gcgttgatag tcgtggtatg aatcacttct ggatcaaatt cgacaaacag gtagttggga
    21301 aacaatggct cactgactgc agtacgtttt ccacgcacga ttttttccag ggtgatcatc
    21361 ggtgccaggc aattcacagc ctgtctttcg aggtgttcct gggcacgttg aagttgcccg
    21421 cgcttgcagt acagtaaata ccaggattgc ataatgactc ttatccgttt aatcggggcg
    21481 caaggatagc aaaagcttta cgctaagtta attatattcc ccggtttgcg ttataccgtc
    21541 agagttcacg ctaatttaac aaatttacag catcgcaaag atgaacgccg tataatgggc
    21601 gtagattaag aggctacaat ggacgccatg aaatataacg atttacgcga cttcctgaca
    21661 ctgcttgaac agcagggtga gctaaaacgt atcacgctcc cggtggatcc gcatctggaa
    21721 atcactgaaa ttgctgaccg cactttgcgt gccggtgggc ctgcgctgtt gttcgaaaac
    21781 cctaaaggct actcaatgcc ggtgctgtgc aacctgttcg gtacgccaaa gcgcgtggca
    21841 atgggcatgg ggcaggaaga tgtttcggcg ctacgtgaag ttggtaaatt attggcgttt
    21901 ctgaaagagc cggagccgcc aaaaggtttc cgcgacctgt ttgataaact gccgcagttt
    21961 aagcaagtat tgaacatgcc gacaaagcgg ctgcgtggtg caccctgcca acaaaaaatc
    22021 gtctctggcg atgacgtcga tctcaatcgc attcccatta tgacctgctg gccggaagat
    22081 gccgcgccgc tgattacctg ggggctgaca gtgacgcgcg gcccgcataa agagcggcag
    22141 aatctgggca tttatcgcca gcagctgatt ggtaaaaaca aactgattat gcgctggctg
    22201 tcgcatcgcg gcggcgcgct ggattatcag gagtggtgtg cggcgcatcc gggcgaacgt
    22261 ttcccggttt ctgtggcgct gggtgccgat cccgccacga ttctcggtgc agtcaccccc
    22321 gttccggata cactttcaga gtatgcgttt gccggattgc tgcgcggtac caagaccgaa
    22381 gtggtgaagt gtatctccaa tgaccttgaa gtgcccgcca gtgcggagat tgtgctggaa
    22441 gggtatatcg aacaaggtga aacggcgccg gaagggccgt atggcgacca caccggttac
    22501 tataacgaag tcgatagttt cccggtattt accgtgacgc atattaccca gcgtgaagat
    22561 gcgatttacc actccaccta tactggtcgt ccgccggatg aacccgcggt gctgggtgtc
    22621 gcactgaacg aagtgtttgt gccgattctg caaaaacagt tcccggaaat tgtcgatttt
    22681 tacctgccgc cggaaggctg ttcttatcgt ctggcggtag tgacgatcaa aaaacagtac
    22741 gccggacacg cgaagcgcgt catgatgggc gtctggtcgt tcttacgcca gtttatgtac
    22801 actaaatttg tgatcgtttg cgatgatgac gtcaacgcac gcgactggaa cgatgtgatt
    22861 tgggcgatta ccacccgtat ggacccggcg cgggatactg ttctggtaga aaatacgcct
    22921 attgattatc tggattttgc ctcgcctgtc tccgggctgg gttcaaaaat ggggctggat
    22981 gccacgaata aatggccggg ggaaacccag cgtgaatggg gacgtcccat caaaaaagat
    23041 ccagatgttg tcgcgcatat tgacgccatc tgggatgaac tggctatttt taacaacggt
    23101 aaaagcgcct gatgcgcgtt tgttttgccc tatttatcga tccgacagag aaagcgcatg
    23161 acaaccttaa gctgtaaagt gacctcggta gaagctatca cggataccgt atatcgtgtc
    23221 cgcatcgtgc cagatgcggc cttttctttt cgcgctggtc agtatttgat ggtagtgatg
    23281 gatgagcgcg acaaacgtcc gttctcaatg gcttcgacgc cggatgaaaa agggtttatc
    23341 gagctgcata ttggcgcttc tgaaatcaac ctttacgcga aagcagtcat ggaccgcatc
    23401 ctcaaagatc atcaaatcgt ggtcgatatt ccccacggag aagcgtggct gcgcgatgat
    23461 gaagagcgtc cgatgatttt gattgcgggc ggcaccgggt tctcttatgc ccgctcgatt
    23521 ttgctgacag cgttggcgcg taacccaaac cgtgatatca ccatttactg gggcgggcgt
    23581 gaagagcagc atctgtatga tctctgcgag cttgaggcgc tttcgttgaa gcatcctggt
    23641 ctgcaagtgg tgccggtggt tgaacaaccg gaagcgggct ggcgtgggcg tactggcacc
    23701 gtgttaacgg cggtattgca ggatcacggt acgctggcag agcatgatat ctatattgcc
    23761 ggacgttttg agatggcgaa aattgcccgc gatctgtttt gcagtgagcg taatgcgcgg
    23821 gaagatcgcc tgtttggcga tgcgtttgca tttatctgag atataaaaaa acccgcccct
    23881 gacaggcggg aagaacggca actaaaactt tattcagtgg catttagatc tatgtcgcat
    23941 ccggcattcg gcacacgatg cctgatgcga cgctggcgcg tcttatcagg cctacattgg
    24001 tgccggatcg gtagaccgga taaggcgttc acgccgcatc cggcaagtgg ttaaacccgc
    24061 tcaaacaccg tcgcaatacc ctgacccaga ccgatacaca tcgtcgccag accaaactgg
    24121 acgtctttgc gctccatcag atttagcagc gtggtgctga tacgcgcacc ggaacagccc
    24181 agcggatgac ccagcgcgat cgcgccaccg ttgaggttga tcttctcgtc aatctgctcc
    24241 attagcccca gatctttaat acatggcagg atctgcgcgg caaaggcttc attcatttca
    24301 aacacgccga tatcactgac agaaagcccc gcttttttca gcgccagctt cgaggccgga
    24361 accgggcagt aacccataat cgatgggtca caaccaacga ccgccatcga acgcacgcgg
    24421 gcgcgcggct taagacctaa ttcatgggcg cggctttcac tcatcaccag catggcagct
    24481 gcgccatcgg aaagcgcaga agatgtaccc gccgttaccg taccgcttac tggatcaaac
    24541 gccggacgca gcgtggcgag ggcttccacg gtggtttccg ggcgaatcac ttcgtcgtaa
    24601 ttaaactgct tcaggacgcc gtcggcatcg tgaccaccgg tcgggataat ttcattttta
    24661 aatgcacccg actgcgtggc ggcccaggcg cgggcgtgtg accgcgcggc aaaggcatcc
    24721 tgcatttcac ggctgatacc gtgcatacgc gccagcattt ctgccgttaa gcccatcatg
    24781 cccgccgctt tggcgacatt gcggctcagg ccggggtgaa aatcgacgcc gtggctcatt
    24841 ggcacatggc ccatatgctc cacgccgccg acaagacatg cctgcgcatc gccagtcatg
    24901 atcattcgtg ctgcgtcatg caatgcctgc atggatgaac cacacaagcg attgacggtc
    24961 accgccggga cagagtgtgg tacttctgcc agcagcgccg cgttacgggc gatattaaaa
    25021 ccctgctcca gcgtctgctg cacacaaccc cagtaaatat cgtcgagggc cgccgcttcc
    25081 agcgccgggt tacgcgccag caggctacgc attaaatgag cggagagatc ttctgcacgc
    25141 acgttacgaa aagcaccgcc cttcgaacgg cccatcgggg tgcgaattgc atcgacaatg
    25201 acaacctgtt ccattgtgac tccttaagcc gttttcaggt cgccaaccgg acgggctggc
    25261 tcaaccgggg gatagtacgg ttcgttatga cgcgctttat tacgcagacc ttccggcact
    25321 tcatacagcg ggccgaggtg ctgatattgc tgtgccatat cgaggtattt tgcgctaccg
    25381 agggtgtcca gccagcggaa cgcgccgccg tggaacggag ggaagcccag gccgtagacc
    25441 agcgccatat ccgcttctgc cggagtggcg ataatgcttt cttccagaca gcgcaccact
    25501 tcgttgacca tcgggatcat catgcgggcg ataatctctt cttcgctgaa atcgcgcttc
    25561 ggctggctca cttctgccag caggtcgtca acgacggcgt cttcttcttt cttcggctta
    25621 cctttgctgc cttctttata acgccagaaa ccgaggccgt tcttctgacc aaagcggttg
    25681 gcatcaaaca gcgcgtcgat ggtatcgcga taatctttct gcatccgctg cgggaagcct
    25741 gctgccatga cagcctgggc atgatgcgcg gtgtcaatgc ccacgacatc cagcagatat
    25801 gccggaccca tcggccagcc aaactgtttt tccattactt tgtcgatctt gcggaaatcc
    25861 gcgccgtcgc gcagcaacag gctgaaaccg gcgaaatacg ggaacagcac gcggttgacg
    25921 aagaagccgg ggcagtcgtt aaccacaatc ggcgttttgc ccatcttgct cgcccaggcg
    25981 acgactttcg cgatggtttc gtcggagctt ttctcgccgc gaattatttc taccaacggc
    26041 attcggtgga ccgggttaaa gaagtgcatc ccgcagaagt tttctggacg ttccagcgcg
    26101 ttggccagtt cgctgatagg aatggttgaa gtgttagacg ccagcacggt attcgggcgt
    26161 actttctgct cggtttctgc cagtacggct tttttcactt ttgggttttc gacaaccgct
    26221 tctaccacaa catccacgcg gtcaaacccg gcgtagtcga gcgttgggtg gattgtggag
    26281 atcacgccag ccagtttcag accatcgatc ttgccgcgct caagctgctt gttcagcagt
    26341 ttcgcggctt cggtcatgcc gagggttaac gacttgtcgt tgatatcttt catgacaacc
    26401 ggcacgcctt tccacgcaga ctggtaagcg atgccgccgc ccataatgcc tgcacccagc
    26461 accgcggcct gtttcggggt ttcaacgtct ttggtgagtt tcttcgcttt gccttttaca
    26521 tattgatcgt taaggaaaat gccgaccagt gcgcgggctt cgttggtatg cgccagcggg
    26581 acaaaacttt tgttttccag gtttaaggct tcttcacgac caaaacgggc cgcagcttca
    26641 atggttttta ctgcggtgat gggggccgga taatgtttcc ccgctgtttg tgcgaccatc
    26701 cctttagcga tggtgaagct catggtggct tcaatcttgc tcagttttag tggttccagc
    26761 ttcggctgac gttttgcttt ccagtcgagg tcgccgttaa tggcctggcg taaaaccgcc
    26821 tttgcgcctt caaccagttt ttctgctttg actacgccat ccaccagacc gattttcagc
    26881 gcctgatccg cgccgacatc tttaccggcg gcaatgattt ccagcgcact gtcagcgccc
    26941 agcatacgtg gcatacgtac agaaccgcca aagcccggca tgatgcccag tttggtttcc
    27001 ggcagaccga tgcgcagatc cggcgtcgcc agacgataat cggtcgccag cacgcattcg
    27061 cagccaccgc ccagcgcata gccgttgacg gcagcaatgg tcggcaccgg cagatcttcc
    27121 aggcgattaa acacgctatt ggcaaagtgc agccactgac ttaactgttc ttcaggaacg
    27181 aggaacaggg acaaaaattc ggtgatatca gcaccgacga taaaggctgc tttgttcgaa
    27241 cgcagcagca gcccttttag atctgattgc tgttccagca cgccgatggc ctcgccgagg
    27301 ctggcgacgg tcgcagtgtc gagtttatta actgaacctg gggcatcaaa taccagttcg
    27361 gcaatgccat cttccagcca gtcaaggtac agggtgtcgc ctttgtaaag catgtcagtc
    27421 tcctgaatcc gcaaggtgat ctggtcgtac cagatgcatc gaagtgtgta ttttgtgtta
    27481 aaaacatgca aataaaagat taaagaaatg ccgatctgat cacgctcggc agaaatcacg
    27541 ctctggatga acgatgtgct aagatgcgga gacttaaggt caaaaaaaca gaagggtaaa
    27601 aaatggaatc actggcctca ctctataaaa atcatatagc taccttacag gaacggactc
    27661 gcgatgcgct ggcgcgcttc aagctggatg cgttacttat tcactccggc gaactgttca
    27721 acgtttttct cgacgatcat ccctatccgt ttaaagtgaa cccgcaattc aaagcgtggg
    27781 tgccggtaac tcaggtgcca aactgctggc tgctggtgga tggcgtgaac aagccgaaac
    27841 tgtggttcta tctgccggtt gattactggc acaacgtcga accgctgccg acctctttct
    27901 ggactgaaga cgtggaagtg atcgcgctgc cgaaagccga tggcattggt agcctgctgc
    27961 ctgctgcgcg cgggaatatc ggttatatcg gtccggtgcc ggaacgtgtg ctgcaactgg
    28021 gtattgaggc cagcaatatc aacccgaaag gggtgatcga ctacctgcat tactaccgct
    28081 ccttcaaaac cgagtacgaa ctggcctgta tgcgtgaagc gcagaaaatg gcggtcaacg
    28141 gtcatcgcgc ggcagaagaa gcgttccgtt ctggcatgag cgagttcgat atcaacattg
    28201 cctatctgac cgcgaccggt catcgtgata ccgacgtacc ttacagcaac attgtggcgc
    28261 tcaacgaaca cgctgcggtg ttgcattaca ccaaactgga ccaccaggca ccggaagaga
    28321 tgcgcagctt cctgctggat gccggggccg aatataacgg ctatgcggct gacctgaccc
    28381 gtacctggtc ggcaaaaagt gacaacgact acgcacagct ggtgaaagac gtaaatgatg
    28441 aacaactggc gctgatcgct accatgaaag caggcgtcag ctatgtggat taccacatcc
    28501 agttccatca gcgcatcgcc aaactgctgc gtaaacatca aatcatcacc gatatgagtg
    28561 aagaagcgat ggtcgaaaac gatctcaccg gaccgtttat gccgcacggt attggccatc
    28621 cgctgggcct gcaggtgcat gacgtagccg gttttatgca ggatgatagc ggtacgcacc
    28681 tcgcggcacc ggcaaaatat ccgtacctgc gctgcacccg tattctccag ccgggcatgg
    28741 tgttaaccat cgaaccgggt atctacttca ttgaatcgct gctggcaccg tggcgtgaag
    28801 ggcagttcag caagcacttc aactggcaga aaattgaagc actgaaaccg ttcggcggca
    28861 ttcgtatcga agacaacgtg gtgatccacg aaaataacgt ggaaaacatg acccgggatc
    28921 tgaaactggc gtgatggaaa gctggttaat tcctgcggca ccggtcacgg tcgttgaaga
    28981 gatcaaaaag agccgcttca taacgatgtt ggcgcatacc gatggcgttg aggcggcgaa
    29041 agcgtttgtt gaatcggtgc gggcagaaca ccccgatgcc cgccaccatt gcgtggcgtg
    29101 ggtggcgggt gcgccggatg attcacaaca gctgggtttc tctgacgacg gggagccggc
    29161 gggaacggca ggtaaaccga tgctcgccca gctaatgggc agcggcgttg gggaaattac
    29221 cgctgtggtg gtgcgctact acggcggcat attgcttggc accggtgggt tagtgaaagc
    29281 gtatggcggc ggcgtgaatc aggcgctgcg ccagctaacg acccaacgca agacgccatt
    29341 aaccgaatat actttgcaat gtgaatatca tcagttaacc ggcattgaag cgttgctggg
    29401 gcagtgtgac ggcaaaatta tcaacagtga ttatcaggca ttcgttctgc tgcgggtggc
    29461 gcttccggcg gcgaaagtgg ctgaattttc cgcaaagctg gcggatttta gccgtggttc
    29521 attgcaattg ttagcgattg aagaataatc cccacttcgt tttgcagaac taaggaagcg
    29581 gcagagatgc attttcgcgc cattacccga atcgttggac tactggtcat cttattttca
    29641 gggaccatga ttatccctgg gctggtagca ctcatctacc gggatggagc gggccgcgct
    29701 tttacccaga ccttttttgt cgccctcgcc attggctcta tgctgtggtg gccgaaccgc
    29761 aaagagaaag gcgaacttaa atcccgtgag gggtttctga tagtggtgct gttctggacc
    29821 gtgctgggta gcgtcggtgc gctccctttt atcttctcgg aaagcccgaa cctcacgatt
    29881 accgatgcgt tttttgaatc tttctctggc ctgaccacca ccggggccac tacgctggtg
    29941 gggctggatt cgctccctca cgccatcctc ttttatcgcc agatgctgca atggtttggc
    30001 gggatgggga tcatcgtgtt agcggttgcg atactgccta tcctcggcgt gggtgggatg
    30061 cagctctatc gcgcagaaat gcccggcccg ctgaaagata acaaaatgcg cccgcgaatt
    30121 gcggaaacgg cgaaaaccct gtggttgatt tatgtcttgc tgaccgtcgc ctgtgcgctg
    30181 gcgttgtggt ttgccggaat ggatgccttt gatgccatcg gccatagctt tgcgactatc
    30241 gctattggcg gcttctcgac acatgatgcc agtatcggtt atttcgatag cccgactatt
    30301 aacactatca ttgctatctt cctgctgatc tccggctgta actacggtct gcacttttca
    30361 ctgttaagtg ggcgtagtct gaaggtttat tggcgcgatc cggaatttcg catgtttatc
    30421 ggcgtacagt ttacgctggt ggttatttgt accctcgtac tgtggtttca taatgtctac
    30481 agttcggcgc tgatgacaat taaccaggcg tttttccagg tggtgtcgat ggcgacaacc
    30541 gccgggttta cgactgacag cattgcccgc tggccgctct ttttgccggt actgctttta
    30601 tgttcggctt ttatcggcgg ttgtgccggg tcaacgggcg gtggcctgaa agtgatccgc
    30661 atcctgctgc tgtttaagca ggggaaccgt gagctgaaac gactggtgca tccgaacgcc
    30721 gtctatagca ttaagctggg gaatcgcgca ctgccggaac gtatcctcga agccgtttgg
    30781 ggatttttct ccgcctatgc attggtgttt attgtcagta tgctggcgat tatcgccacg
    30841 ggcgtggatg acttttctgc ctttgcctca gttgttgcga cattgaataa cctggggcca
    30901 gggcttggcg tggttgctga taactttacc agtatgaacc cggtggctaa atggatcctg
    30961 attgccaaca tgctgtttgg tcgtctcgag gtctttacat tgctggtgct ctttaccccg
    31021 actttctggc gtgaatgatg gagtaatacg tgaaaacatt aattcttttc tcaacaaggg
    31081 acggacaaac gcgcgagatt gcctcctacc tggcttcgga actgaaagaa ctggggatcc
    31141 agacggatgt cgccaatgtg caccgcattg aagaaccaca atgggaaaat tatgaccgtg
    31201 tggtcattgg tgcttctatt cgctatggtc actaccactc tgcgttccag gaatttgtca
    31261 aaaaacatgc gacgcgactg aattcgatgc cgagcgcctt ttattccgta aacctggtgg
    31321 cgcgcaaacc ggagaagcgt actccacaga ccaacagcta cgcgcggaag tttctgatga
    31381 actcgcaatg gcgtcccgat cgctgcgcgg tcattgccgg ggcgctgcgt tacccacgtt
    31441 atcgctggta cgaccgtttt atgatcaagc tgattatgaa gatgtcaggc ggtgaaacgg
    31501 atacgcgcaa agaagttgtc tataccgatt gggagcaggt ggcgaatttc gcccgagaaa
    31561 tcgcccattt aaccgacaaa ccgacgctga aataagcaca aagaataaaa aatgcgcggt
    31621 cagaaaatta ttttaaattt cctcttgtca ggccggaata actccctata atgcgccacc
    31681 actgacacgg aacaacggca aacacgttgc cgggtcagcg gggttctcct gagaatctca
    31741 acagagaaaa gcaa
//
LOCUS       DAZGVQ010000044        31744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000044 DAZGVQ010000000
VERSION     DAZGVQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.044"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(172..1545)
                     /gene="argH"
                     /locus_tag="ACHLAG_001958"
     CDS             complement(172..1545)
                     /gene="argH"
                     /locus_tag="ACHLAG_001958"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFP0655199.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSHVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1606..2382)
                     /gene="argB"
                     /locus_tag="ACHLAG_001959"
     CDS             complement(1606..2382)
                     /gene="argB"
                     /locus_tag="ACHLAG_001959"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFP0655200.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLSVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(2390..3394)
                     /gene="argC"
                     /locus_tag="ACHLAG_001960"
     CDS             complement(2390..3394)
                     /gene="argC"
                     /locus_tag="ACHLAG_001960"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFP0655201.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3548..4699
                     /gene="argE"
                     /locus_tag="ACHLAG_001961"
     CDS             3548..4699
                     /gene="argE"
                     /locus_tag="ACHLAG_001961"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFP0655202.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            5051..7702
                     /gene="ppc"
                     /locus_tag="ACHLAG_001962"
     CDS             5051..7702
                     /gene="ppc"
                     /locus_tag="ACHLAG_001962"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFP0655203.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7885..9618
                     /gene="eptC"
                     /locus_tag="ACHLAG_001963"
     CDS             7885..9618
                     /gene="eptC"
                     /locus_tag="ACHLAG_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFP0655204.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9767..10618
                     /gene="yijO"
                     /locus_tag="ACHLAG_001964"
     CDS             9767..10618
                     /gene="yijO"
                     /locus_tag="ACHLAG_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0655205.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10605..10946)
                     /gene="frwD"
                     /locus_tag="ACHLAG_001965"
     CDS             complement(10605..10946)
                     /gene="frwD"
                     /locus_tag="ACHLAG_001965"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP0655206.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRVDVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10948..11826)
                     /gene="pflC"
                     /locus_tag="ACHLAG_001966"
     CDS             complement(10948..11826)
                     /gene="pflC"
                     /locus_tag="ACHLAG_001966"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312908.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFP0655207.1"
                     /translation="MTSSAGQRISCNVVETRRNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVNMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11792..14089)
                     /gene="pflD"
                     /locus_tag="ACHLAG_001967"
     CDS             complement(11792..14089)
                     /gene="pflD"
                     /locus_tag="ACHLAG_001967"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP0655208.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14140..14460)
                     /gene="frwB"
                     /locus_tag="ACHLAG_001968"
     CDS             complement(14140..14460)
                     /gene="frwB"
                     /locus_tag="ACHLAG_001968"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP0655209.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14475..15554)
                     /gene="frwC"
                     /locus_tag="ACHLAG_001969"
     CDS             complement(14475..15554)
                     /gene="frwC"
                     /locus_tag="ACHLAG_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFP0655210.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15863..18364
                     /gene="ptsP"
                     /locus_tag="ACHLAG_001970"
     CDS             15863..18364
                     /gene="ptsP"
                     /locus_tag="ACHLAG_001970"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP0655211.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKDMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18376..19038
                     /gene="fsa"
                     /locus_tag="ACHLAG_001971"
     CDS             18376..19038
                     /gene="fsa"
                     /locus_tag="ACHLAG_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP0655212.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            19049..20152
                     /gene="gldA"
                     /locus_tag="ACHLAG_001972"
     CDS             19049..20152
                     /gene="gldA"
                     /locus_tag="ACHLAG_001972"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFP0655213.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALEHVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPEAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            20427..21044
                     /gene="yijF"
                     /locus_tag="ACHLAG_001973"
     CDS             20427..21044
                     /gene="yijF"
                     /locus_tag="ACHLAG_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFP0655214.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(21071..21976)
                     /gene="yijE"
                     /locus_tag="ACHLAG_001974"
     CDS             complement(21071..21976)
                     /gene="yijE"
                     /locus_tag="ACHLAG_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFP0655215.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(22070..24250)
                     /gene="katG"
                     /locus_tag="ACHLAG_001975"
     CDS             complement(22070..24250)
                     /gene="katG"
                     /locus_tag="ACHLAG_001975"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFP0655216.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24579..25469)
                     /gene="metF"
                     /locus_tag="ACHLAG_001976"
     CDS             complement(24579..25469)
                     /gene="metF"
                     /locus_tag="ACHLAG_001976"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFP0655217.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            25545..25679
                     /locus_tag="ACHLAG_001977"
     CDS             25545..25679
                     /locus_tag="ACHLAG_001977"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655218.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(25818..28250)
                     /gene="metL"
                     /locus_tag="ACHLAG_001978"
     CDS             complement(25818..28250)
                     /gene="metL"
                     /locus_tag="ACHLAG_001978"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFP0655219.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28253..29413)
                     /gene="metB"
                     /locus_tag="ACHLAG_001979"
     CDS             complement(28253..29413)
                     /gene="metB"
                     /locus_tag="ACHLAG_001979"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFP0655220.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29690..30007
                     /gene="metJ"
                     /locus_tag="ACHLAG_001980"
     CDS             29690..30007
                     /gene="metJ"
                     /locus_tag="ACHLAG_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFP0655221.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30191..30799
                     /gene="yiiX"
                     /locus_tag="ACHLAG_001981"
     CDS             30191..30799
                     /gene="yiiX"
                     /locus_tag="ACHLAG_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFP0655222.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            30944..31619
                     /locus_tag="ACHLAG_001982"
                     /pseudo
     CDS             30944..31619
                     /locus_tag="ACHLAG_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         7658 a         8836 c         8054 g         7196 t
ORIGIN      
        1 tgataaaaag gaatgtgaca aataatggtt ctggttgggc gttatattta aggtcaccag
       61 aatgctgaca aatttctgcc tgatgtggcg taaacgcctt atccgcctac gaagtgtcgc
      121 actttgtagg cgtggtaagc tctccatttt caggggagta atataaacaa attactctaa
      181 ccgagcctgc gcaaaagcaa tcgcctgcgc cacctgctgc ggtgagacgc cgccttttgc
      241 cgcacgcttg tcgaggcacg attgtagcga cagaatcgga tagacatctt cgccaatcac
      301 gtggctgaat ttctgcaact cactgagagg cagatcttcc agcggtttgc cctgacgaat
      361 ggcttccacc accgcttcac caacaatatg gtgcgcctcg cggaacggta cgcctttcgc
      421 caccagataa tccgccagtt cggtggcgtt ggcgtaaccc tgctgagccg cttcttggca
      481 acgtggacgt ttcacctgaa tgccgtccag caccagcgcc gccatatgca ggcagtccag
      541 ccaggtatcg agcgcgtcga acagaccttc tttgtcttcc tgcatatctt tgttgtaagc
      601 cagcggcaaa cctttcagcg tcatcatcat gccggttaac gccccctgca cccgaccgca
      661 tttaccgcga atcagctcca gcgcatccgg gtttttcttc tgcggcatta atgatgaacc
      721 ggaagtcacg cggtcagaaa gctccacaaa ccccgcttcg ccggtgttaa agaaaatcag
      781 atcttcagca aaacgcgaca gatgcaccat gccgatagcg gcagccgaca gcagttccaa
      841 cacatggtca cggtcagaaa cgctgtccag actgttacgg gtcgccgaag caaagcccag
      901 ccagcctgct aactgttcac ggtcgatttc ataggccgtt cccgccagcg cgccacagcc
      961 taatgggctg acatccagac gcttaagcgc atcctgcaaa cggctttcat cacgcgccag
     1021 catctcaaca taggccaggc accagtgcgc gaacgtcacc ggctgggcgc gttgcaggtg
     1081 agtgtaacct ggcatgaccg cgtcctgatt gttttgtgcg gtttccacca gcgccgattg
     1141 cagctgtcgg ttagccgtca gtaactcgct aacggtatct ttgcaccaca gtttcaggtc
     1201 agtcgctacc tgatcgttac gactacgccc ggtatgcagc tttttgccta actggcccac
     1261 tttgtcgatc agtttgcctt ccacccagct atggatatct tcggcgtcgc tttcaaggat
     1321 ttgttgtggc ctggcgcgaa catcttccaa cagcacgttc agcgcctctt ccagttgcgc
     1381 ctgctcttct gcggttaaca cgcctaccgt gaccagggct ttggaccagg ccacagagcc
     1441 aacaatatcc tgctccgcca gacggtaatc aaagcgcagt gagtcgttga attgtttgaa
     1501 ccgttgatct gctgcctggg taaaacgccc gccccaaagt gccataactc tgtttcctta
     1561 ttttgaaatt caatgccgga aagctgcgcc tccggccaac aaaacttaag ctaaaatccg
     1621 cgtacccatc ggcataccgt taaacagtgc cggaagctgc tccgcatgac gccaggaggc
     1681 gatatctacc ggacggccca gcgtgcgggc cgcatccagc gccgcgttca ctttcactat
     1741 catgccgtca gtaataatgc cctgctcaat cagttgttct gctttcgcgg cggtcatttc
     1801 ggcaatgcgt tgccctttgc cgtcgagaat gccgctgacg tcggagagca aaatcagatc
     1861 cgcgcccagc gttgccgcca gcgccgttgc cgcctggtcg gcattgacgt tcatcagttg
     1921 cccttcgtct gttacgccaa tggagctgac caccggcaga taaccgttct ccagcaagga
     1981 gttgataagc ttaggcgaac ctggctgcgc cagtccaaca tgacctaact cttcatcaag
     2041 ctgggtcact ttgacgctgt cgccgtcacc gagaaacaaa cctacggccg caatctgatg
     2101 tttcttcgcc catgccaaca gggttttatt tgccgttccc gccagtgctc cggtgataat
     2161 gtctatctga tcagcaggcg tcacccgcag gccgtttttc tttttcaccg acagattcag
     2221 ccctttcatc agctcatcca ccacgcaacc gccgccgtgc acaatcacca gcggacgctg
     2281 atgtgactca cgataattca ccagtgcgct aaacagacgt tccagcgcct cttcactatc
     2341 cagcagtacg ccgcccagtt tgataattaa tggattcatc attgcaccct taaataagag
     2401 actgcgtttc cgcatagccg aaacgaatat tggcgcactg taccgcttgt gccgccgcgc
     2461 ctttcagtaa gttgtcttcg gtcgccacaa taatcagatg ctcgccctga acggcaaacc
     2521 cgatatcgca aaatggcagc ccaacgacat ttttcagcgc cggaacgcct ttgtcataca
     2581 gccgcaccag cggtttatgg gcatacgcct gctgtaacac ttgcgcgact tgcgcctggg
     2641 tcacacccga tttcaggcgg caggtaatgg tttcgagaat gccgcgcggg aaattgccca
     2701 gatgcggagt gaagataacg tcagcaccga ggtgtgtggc gatctctggt tgatggcgat
     2761 gagtaaagac gccatacggt tgcaggctaa cttcacaaaa actatttgaa atcgctgcct
     2821 tacgtcctgc accgctcacg ccgctggtgg cgttgatcac cggccactga ttgaggtcaa
     2881 gaagatcggc atcaatcaac ggtttcagcg ccagctgtgc cgccgtcgga taacagcctg
     2941 gcaccgcaat caaattcgct tcttttaatt tattaccgca ccactccgcc agaccgtagg
     3001 ctgcctgttc cagcagttcc gggtattgat gggtaaagcc gtaatatttt tcatagaagg
     3061 tggcgtcgtt aacacgaaac gcgccggaaa ggtcgaacac cacgcagccc gcttcaagaa
     3121 actgcggcgc taaatcgtgg ctaacttcat gggcggtggc gagaaacact acgtccaccc
     3181 ctgggctaaa ctcgctgata tccgacatcg gctgcaacgg cagatcaacg atgcctttta
     3241 gctgcggatg caaatcggag attaactttc ccgcatcatt gctttgcgct gaaacagtca
     3301 aagcggttat gttcatatgc ggatggcgat ttacataggt cactagctct gcgccagcgt
     3361 agccgctggc acccacaatc agcgtattca acatcggggc tattcacctt cttatgtctg
     3421 gttgccaggt taaacgtaaa acattcacct tacggctggt gggttttatt acgctcaacg
     3481 ttagtgtatt tttattcata aatactgcat gaatattgat actatcatga ccagaggtgt
     3541 gtcaacaatg aaaaacaaat taccgccatt tatcgagatt taccgtgctc tgattgccac
     3601 accttcaata agcgccacgg aagaggcact cgatcaaagc aatgcagatt taatcactct
     3661 gctggcggac tggtttaaag atttgggctt caatgtggaa gtgcagcctg tgccaggaac
     3721 tcgcaacaaa ttcaatatgc tggcaagtat cggacagggg gctggcggct tgttgctggc
     3781 ggggcatacc gatacggtgc catttgatga cggtcgctgg acgcgcgatc cgtttacact
     3841 gacggagcat gacggcaagc tttacggctt aggcaccgcc gacatgaaag gcttttttgc
     3901 gtttatcctt gatgcgctac gcgatgtcga cgtcacgaaa ctgaaaaaac cgctctacat
     3961 tctggcgact gctgatgaag aaaccagtat ggccggagcg cgttattttg ccgaaactac
     4021 cgccctgcgc ccggattgcg ccatcattgg cgaaccgacg tcactacaac cggtacgcgc
     4081 acataaaggt catatctcta acgccatccg tattcagggc cagtcggggc actccagcga
     4141 tccagcacgc ggagttaacg ctatcgaact aatgcacgac gccatcgggc atattttgca
     4201 attgcgcgat aacctgaaag aacgttatca ctacgaagcg tttaccgtgc cataccctac
     4261 gctcaacctc gggcatattc acggtggcga cgcttctaac cgtatttgcg cttgctgtga
     4321 gttgcatatg gatattcgtc cgctgcctgg catgacactc aatgaactta atggtttgct
     4381 caacgatgca ttggctccgg tgagcgaacg ctggccgggt cgtctgacgg tcgacgagct
     4441 gcatccgccg atccctggct atgaatgccc accgaatcat caactggttg aagtagttga
     4501 gaaattgctc ggagcaaaga ccgaagtggt gaactactgt accgaagcgc cgtttattca
     4561 aacgttatgc ccgacgctgg tgttggggcc tggctcaatt aatcaggctc atcaacctga
     4621 tgaatatctg gaaacacggt ttatcaagcc cacccgcgaa ctgataaccc aggtaattca
     4681 ccatttttgc tggcattaaa acgtaggccg gataaggcgc tcgcgccgca tccggcaccg
     4741 ttgccaaact cctgtgccgc aataatgtcg gatgcgatgc ttgcgcatct tatccgacct
     4801 acacctttgg tgttatgtat ggcgattttt taacatttcc ataagttacg cttatttaaa
     4861 gcgtcgtgaa tttaatggcg taaattcctg ctatttattc gtttgctgaa gcgatttcgc
     4921 agcatttgac gtcaccgctt ttacgtggct ttataaaaga cgacgaaaag caaagcccga
     4981 gcatattcgc gccaatgcga cgtgaaggat acagggctat caaacgataa gatggggtgt
     5041 ctggggtaat atgaacgaac aatattccgc attgcgtagt aatgtcagta tgctcggcaa
     5101 agtgctggga gaaaccatca aggatgcgtt gggagaacac attcttgaac gcgtagaaac
     5161 tatccgtaag ttgtcgaaat cttcacgcgc tggcaatgat gctaaccgcc aggagttgct
     5221 caccacctta caaaatttgt cgaacgacga gctgctgccc gttgcgcgtg cgtttagtca
     5281 gttcctgaac ctggccaaca ccgccgagca ataccacagc atttcgccga aaggcgaagc
     5341 tgccagcaac ccggaagtga tcgcccgcac cctgcgtaaa ctgaaaaacc agccggaact
     5401 gagcgaagac accatcaaaa aagcagtgga atcgctgtcg ctggaactgg tcctcacggc
     5461 tcacccaacc gaaattaccc gtcgtacact gatccacaaa atggtggaag tgaacgcctg
     5521 tttaaaacag ctcgataaca aagatatcgc tgactacgaa cacaaccagc tgatgcgccg
     5581 cctgcgccag ttaatcgccc agtcatggca taccgatgaa atccgtaagc tgcgtccaag
     5641 cccggtagat gaagccaaat ggggctttgc cgtagtggaa aacagcctgt ggcaaggcgt
     5701 accaaattac ctgcgcgaac tgaacgaaca actggaagag aacctcggct acaaactgcc
     5761 cgtcgaattt gttccggtcc gttttacttc gtggatgggc ggcgaccgcg acggcaaccc
     5821 gaacgtcact gccgatatca cccgccacgt cctgctactc agccgctgga aagccaccga
     5881 tttgttcctg aaagatattc aggtgctggt ttctgaactg tcgatggttg aagcgacccc
     5941 tgaactgctg gcgctggttg gcgaagaagg tgccgcagaa ccgtatcgct atctgatgaa
     6001 aaacctgcgt tctcgcctga tggcgacaca ggcatggctg gaagcgcgcc tgaaaggcga
     6061 agaactgcca aaaccagaag gcctgctgac acaaaacgaa gaactgtggg aaccgctcta
     6121 cgcttgctac cagtcacttc aggcgtgtgg catgggtatt atcgccaacg gcgatctgct
     6181 cgacaccctg cgccgcgtga aatgtttcgg cgtaccgctg gtccgtattg atatccgtca
     6241 ggagagcacg cgtcataccg aagcgctggg cgagctgacc cgctacctcg gtatcggcga
     6301 ctacgaaagc tggtcagagg ccgacaaaca ggcgttcctg atccgcgaac tgaactccaa
     6361 acgtccgctt ctgccgcgca actggcaacc aagcgccgaa acgcgcgaag tgctcgatac
     6421 ctgccaggtg attgccgaag cgccgcaagg ctccattgcc gcctacgtga tctcgatggc
     6481 gaaaacgccg tccgacgtac tggctgtcca cctgctgctg aaagaagcgg gtatcgggtt
     6541 tgcgatgccg gttgctccgc tgtttgaaac cctcgatgat ctgaacaacg ccaacgatgt
     6601 catgacccag ctgctcaata ttgactggta tcgtggcctg attcagggca aacagatggt
     6661 gatgattggc tattccgact cagcaaaaga tgcgggagtg atggcagctt cctgggcgca
     6721 atatcaggca caggatgcat taatcaaaac ctgcgaaaaa gcgggtattg agctgacgtt
     6781 gttccacggt cgcggcggtt ccattggtcg cggcggcgca cctgctcatg cggcgctgct
     6841 gtcacaaccg ccaggaagcc tgaaaggtgg cctgcgcgtg actgaacagg gcgagatgat
     6901 ccgctttaaa tatggtctgc cagaaatcac cgtcagcagc ctgtcgctgt acaccggggc
     6961 gattctggaa gccaacctgc tgccaccacc tgagccgaaa gagagctggc gtcgcattat
     7021 ggatgaactg tcagtcatct cctgcgatct ctaccgtggc tacgtacgtg aaaacaaaga
     7081 ttttgtgcct tacttccgct ccgctacgcc ggaacaagaa ttgggcaaac tgccgttggg
     7141 ttcacgtccg gcgaaacgtc gcccaaccgg cggcgtcgag tcgctgcgcg ccataccgtg
     7201 gatttttgcc tggacgcaaa accgcctgat gctccccgcc tggctgggtg caggtacggc
     7261 gctgcaaaaa gtggtcgaag atggcaaaca gagcgagctg gaagccatgt gccgcgattg
     7321 gccattcttc tcgacgcgtc tcggcatgct ggagatggtc ttcgccaaag cagacctgtg
     7381 gctggcggaa tactatgacc aacgcctggt agacaaagca ctgtggccgt taggtaaaga
     7441 gttacgcaac ctgcaagaag aagacatcaa agtggtgctg gcgattgcca acgattccca
     7501 tctaatggcc gatctgccgt ggattgcaga gtctattcag ctacggaata tttacaccga
     7561 cccgctgaac gtattgcagg ccgagttact gcaccgctcc cgccaggcag aaaaagaagg
     7621 ccaggaaccg gatcctcgcg tcgaacaagc gttaatggtc actattgccg ggattgcggc
     7681 aggtatgcgt aataccggct aatcttcctc ttctgcaaac cctcgtgctt ttgcgcgagg
     7741 gttttctgaa atacttctgt tctaacaccc tcgttttcaa tatatttctg tctgcatttt
     7801 attcaaattc tgaatatacc ttcagatatc cttaaggaat tgtcgttaca ttcggcgata
     7861 tttttttcaa gacaggttct tactatgcat tccacagaag tccaggctaa accccttttt
     7921 agctggaaag ccctgggttg ggcactgctc tacttttggt ttttctctac tctgctacag
     7981 gccattattt acatcagtgg ttatagtggc accaacggca ttcgcgactc gctgttattc
     8041 agctcgctgt ggttgatccc ggtattcctc tttccgaagc ggattaaaat tattgccgca
     8101 gtaatcggcg tggtgctatg ggcggcctct ctggcggcgc tatgctacta cgtcatctac
     8161 gggcaggagt tctcgcagag cgttctgttt gtgatgttcg aaaccaacac caacgaagcc
     8221 agcgagtatt taagccagta tttcagcctg aaaattgtgc ttatcgcgct ggcctatacg
     8281 gcggtggcag ttctgctgtg gacacgcctg cgcccggtct atattccaaa gccgtggcgt
     8341 tatgttgtct cttttgccct gctttatggc ttgattctgc atccgatcgc catgaatacg
     8401 tttatcaaaa acaagccgtt tgagaaaacg ttggataacc tggcctcgcg tatggagcct
     8461 gccgcaccgt ggcaattcct gaccggctat tatcagtatc gtcagcaact aaactcgcta
     8521 acaaagttac tgaatgaaaa taatgccttg ccgccactgg ctaatttcaa agatgaatcg
     8581 ggtaacgaac cgcgcacttt agtgctggtg attggcgagt cgacccagcg cggacgcatg
     8641 agtctgtacg gttatccgcg tgaaaccacg ccggagctgg atgcgctgca taaaaccgat
     8701 ccgaatctga ccgtgtttaa taacgtagtt acgtctcgtc cgtacaccat tgaaatcctg
     8761 caacaggcgc tgacctttgc caatgaaaag aacccggatc tgtatctgac gcagccgtcg
     8821 ctgatgaaca tgatgaaaca ggcgggttat aaaaccttct ggatcaccaa ccagcagacg
     8881 atgaccgccc gcaataccat gctgacggta ttttcgcgcc agaccgacaa gcagtactac
     8941 atgaaccagc aacgtacgca gagtgcgcgt gaatacgaca ccaacgtgct gaagccgttc
     9001 caggatgtgc tgaatgaccc tgcgccgaag aaactgatca tcgttcatct gctgggtacg
     9061 catatcaaat acaaataccg ctacccggaa aatcagggca agtttgatgg caataccgat
     9121 catgttccgc caggattaag cgcagaagag ctggaatcat ataacgatta tgacaacgct
     9181 aacctgtata acgatcatgt ggttgccagc ctgattaaag actttaaagc ggcagacccg
     9241 aacggattcc tggtttactt ctctgaccac ggtgaagagg tttacgacac gccgccgcac
     9301 aaaactcagg ggcgtaacga agacaacccg acgcgccaca tgtacaccat tccgttcctg
     9361 ctgtggacgt cggaaaaatg gcaagcgact catccccgtg atttctcaca ggatgtcgat
     9421 cgtaaataca gcctggcgga actgatccac acctggtcag atttggcggg cttatcttac
     9481 gacggttacg atccaacccg ttcagtggtg aatccgcagt tcaaagaaac tacccgctgg
     9541 attggtaacc cgtacaagaa aaacgcgctg atcgattacg acactctgcc gtatggcgac
     9601 caggtaggta atcagtaaat gacctttgta tcgcgtctgg cgctatttgc cggatgcgat
     9661 actgccgcaa tgtttacatc gaactcccct caggccacac ttttcaggtg gcctttttta
     9721 ataatgccag ttactgtttt cccgtctaca tacctaaggg aataacatgt atcacgacgt
     9781 cagctatctg ctctcccgcc tgatcaacgg cccgctgtcc ctgcgccaga tttactttgc
     9841 cagcagtaac ggcccggtgc ccgatcttgc gtatcaggtc gattttccac ggctggaaat
     9901 tgtgctggaa ggtgagtttg ttgataccgg cgctggagca acgttagttc ccggcgatgt
     9961 gctgtacgtt gccgctggtg gctggaattt cccacaatgg aaaacacccg ctaccacctt
    10021 tagcgtgctg tttggcaaac agcaactcgg cttcagcgtc gtgcaatggg atggcaaaca
    10081 atatcaaaat ctggcgaagc aacacgtcgc ccgacgcggc ccacgtatag gttcttttct
    10141 gctacaaacg ctcaatgaaa tgcaaatgca gccgcaggag cagcaaacgg caaggcttat
    10201 cgtagccagc ctgcttagcc actgccgcga tttgctcggc agccaaatcc agaccgcctc
    10261 acgcagccag gcactatttg aagctattcg cgattatatc gacgaacgct atgcctccgc
    10321 gcttacccgc gaatctgtcg cacaggcatt ctatatttca ccaaactatc tctcgcacct
    10381 gtttcaaaaa acgggggcca ttggttttaa cgaatacctg aatcacacgc gactggagca
    10441 cgctaagacg ttattgaaag gctatgacct gaaggtaaaa gaagtggcgc acgcctgcgg
    10501 ctttgtcgac agcaactact tctgccgtct gtttcgtaaa aacaccgaac gctcgccgtc
    10561 ggaatatcgc cggcagtacc acagccaact gacagaaaaa ccgactactc cagaataaga
    10621 tgggtttgct gcggtgcaga aagcactttg cgcaccgcgc tcattacccg ctgcggctca
    10681 cgcaggaatg cgtagatgct gcattgcaca tagcggcaat gttcaaatcg ctcggcaccg
    10741 gcaagctcga tatccgtaat caacagagca acatccaccc gacggatatc ctcgtccgct
    10801 aaacgattct ccgttcccaa cgcgccctga gtttcaatgc tgacgcccca cttctctaac
    10861 tggcacaact tttccagccg ttccgccgcc atatatgtat gcgccacgcc gcttacgcag
    10921 gcggttactg ccaccaggta tgccatttta acctcccacg gtaacctgaa atccggcccg
    10981 ttctgccatt tcgcgcatcg ttgtcacatc ggctgacgac ggcgcaggca cctctttcat
    11041 cgaccatgtt ttccccagca ggcggtattt cggttcgccg tactgatgaa acggtaacag
    11101 atggatctgc ctgatattca gcgggatcag cacgtccagc gcctgctgca tattctcccg
    11161 gctgagcgtg aaaccaggga tcagcggtaa acgcgggatc acgttgacgc cctcactcac
    11221 cagcaaacgc agattctcca gcacgcgtgg caggttcata ttcaccacat cccgcgcctg
    11281 agtcgcatcc ataattttta aatcgaacaa cacttcatcg cacaatttcg ccagtggtaa
    11341 cagcttgctg gccggcgcgt ctccggcagt ttcaatggca catgagacac cccacagccg
    11401 cagtcgctgt aaaaaacggg tagcaaactc cgcctgcatt aacacttcgc cgccagaaag
    11461 cgtgacgccg ccgccggacg tgcgaaagaa aatgtcatct ttcatcactt cccgttccag
    11521 agcgtcaagg ctgatatcgc gaccaatccg ttcaaacgca ccggaggggc attcatccgc
    11581 atcacgcaaa catttcgcac agtgcagaca tttcgcctct ctgcgtaccg tctggatttt
    11641 gccggagatc gactccggat tagcacacca cgggcaaaga tgcggacagc ctttaaaaaa
    11701 gaccaccgta cgaatgccct cgccgtcatt cagtgaataa cgctgaatgt tgaaaatgcg
    11761 cgccacatca ttgcggcgcg tttccacaac gttacagctg atgcgctgtc cggcggatga
    11821 tgtcatcctg gatctccttc gacagttcga caaagaaggc gctgtatccg gcaacgcgca
    11881 ccaccagccc ggcataatct tgtgggcgct gttgcgcttc ccgcaacgtg tcggcgttca
    11941 ccacgttaaa ctgaatatgc tgcaacttaa gctgggtaaa cgcccgtaag aagtcggcca
    12001 gtttgcgtaa tcccgcttca ccttccaggg tcgccggagt gaatttcacg ttcagcaacg
    12061 taccgttaga cagcagcgta ttatcgagct tactgactga cttcagtacc gctgttggcc
    12121 cttgtgcgtc ctggcccagc ataggtgaca agccgccgtc tgccagttgt tccccggcaa
    12181 aacgaccgtc tggcgtcgcg ccaaccaccg atcccaacgg gacgtgagca gaaacggtat
    12241 acgatcccgg cgtgaagtaa ccgccgcgcg ggttctggta tttttccact tctttgcagt
    12301 agtggcgtaa cagttcggcg ctgatgttat ccacctcgtc gatatcgtta ccgtatttct
    12361 cgaagcggtt aattaagcga gcacggactt tttcgccttc tggcgttgca aagttggctt
    12421 ttaataccga cagcaattcg tcaaaactta aacgctgttg ctcaaaaacc atccctttca
    12481 acgcatggag agaatcgctc aggttggcga taccgatccc ctgtacgccg gagaagttat
    12541 aacgcgcgcc gccatcggta atatcgcgac ctttttccag acaatcgctg ataaaagacg
    12601 agagcagcgg tacaggtgcc cagtcgcgat ggccgatatc acaaatattg ctgccctcaa
    12661 ccatcagggt gatgtagtgg ctgatcttgg cgcggatctg ctccagtaaa ccttcataag
    12721 tcagcgcagc attgccttca ttttcatgca ggcagatttc catcactttc agcagattaa
    12781 acatcgcgat gtcatgcaag ccgtaggttc tgccgggaat agataattcc acacagccca
    12841 ctacggaata gtcgcgcgca tcttccagcg aaacgccacg gttgaggaac gctggtacca
    12901 ccacttcatc gttaaagatt tgcggaatac cggtgccgag gcgaatggtt tcggcggttt
    12961 tcatcaggaa cggcgtgtcg atcaaggcgt tagtgcgcac gccgaggttc ggttgcggta
    13021 attgcacgct ttgataagcg tcaaggcaaa ggaacgaaag cacgttcacc gcgctacgtc
    13081 cgttctcggt taacccgccg agcagtgcgg tatagccggt cgggaaacct gcgaaataac
    13141 gcgcgctgct ggtggagcgc aacagcacga tgtcgttgca tttcacccat aaagattcga
    13201 gtagttcttt caggaacgcc ggatcttcgc cctgggttaa agatgcctga tagaacggca
    13261 acatatactg gtcgaagcgc cccaacgata gcgaactggc gttggattcg tattgcagaa
    13321 tgatgttcat gtaccagaat aactggcacg cctgccagaa cgtctgcggc ttatgttcgg
    13381 cgttatggcg ggagatctcc gcaatagtca gcagttcttc gcgacgctgg gcatctgtgc
    13441 agtttgccgc cattgtttcc gccagttcag cgtaacgcag aatgtgtttc tgcgaggctt
    13501 ccagcagtaa cagcgcggcc tgataaaagt gattctccgg ctgttgctga caatgttgct
    13561 gcatctgcgc caccagctcc cccagcccgt gattcagcag gcgtgggtaa tcaataataa
    13621 tgtgcccctg gcctttatct gtctggttga tgctgaaaat ctgcgtgctg gtcgcggctt
    13681 ttacttcatc tgtcatctgc ccgttgatga aatctttcat cgaacgtttt tcccagtacg
    13741 ggaacaactc ttcacggtag atacgtttat cttcttcact gatagcaaag cggtcctgcg
    13801 gacgcgtcgg gaattgatcc agctctttca gcagccagta agggtccatt tccggcgaca
    13861 taatcccagc gcgcggtttt acggtgcggt taccggcaat cagttcttca tcacgaatcg
    13921 aaatttcaac atgttcaagg atatacgctg ttgctttcgc ccggcgcaat atcaccggtt
    13981 cgccttcggt ttgccgatgg ctggcggtat aaagcagcgc ccgctccagc gagatttcac
    14041 gggtattggc aaacagtgca gttttgaggc gagagatacg attcgtcatg aggaactcct
    14101 gtgggaaagc gaacccgccc ttgcgagcgg gagaagaact taagcggttt gcgccaggtg
    14161 agcttcaatt ttgctcataa tggcgtcagc acgttttact gcatcgctga tgttgacgcg
    14221 cacgatggtc ttgccagcaa aacgctcttc aaacttgatg ccgatatctt tggtcagaat
    14281 caccatatca gcgctcgcca catcttccgc agtcagttca ttttccagac caattgaccc
    14341 ctgggtttct actttcactt cccagccttt cgctttcgcg gcactttcca gcgcctctgc
    14401 tgccatatag gtatgggcaa cacctgaagg acatgcggtt actgcaataa ttttcgtcat
    14461 ggctcgttcc tcaattaatt aatttcaaaa tccaaatcca ggtcgtcttc tttttcatca
    14521 gtcgaagacc catttttacg cgccagactt ttcagcacgt tcacacaaac agccgtcacc
    14581 actgctccga cagccactgc tgcgatataa cccagcttgc cttcaaccac cggcagcaca
    14641 atcagaccac cccagcctgc atagcactgc gcaccgacca gcgccgcagt tactgcacca
    14701 caaactgaac cgaccatgat ggaaggaatg acacgcagcg gatcggcagc ggcgaaagga
    14761 atcgcccctt cagtaacgcc aacacagccc atcaccagtg ccgctttgcc ggtttcgcgc
    14821 tcttctgcgg agaaattttt acgaccaatc agcgtcgcca gccccatccc cagcggcggg
    14881 atacaaatac ccacggcagc gatggccacc acggtataaa caccctgagc aacgcaaatc
    14941 agcatgaagg cataggccac tttgttaacc ggaccgccca tatcgaacgc cagcatcaga
    15001 cccatgatca ccgccagcat aacaatgctg ccctgctgca tcccctgaag ccactgagtc
    15061 aggctgttgg tcaacgcccc tacaggctcg cccaagcccc acatcatgat gcctgcggta
    15121 atcagtgtgc cgacgatagg aataatgaag ataggcatca ccgagcgcag aactttatgc
    15181 accggaattt tcttcaggta atgcaccacg atgccgccga taatcccggc gatcagtgca
    15241 ccaaagaagc ccgcaccaaa gctgttacca acccaggcac cgatagcgca cggagccagc
    15301 gcagaacgct ctgcaatgga ataaccgatg tacgctgcga ggaaaggcac catcagcgtc
    15361 aagcccgcaa cgccgatatc aaacagtttt ttcagatttg gatcggctac ggcatccggc
    15421 actgcgcctt tgccatacaa catgacggaa accgccagca aaataccgcc cgataccacg
    15481 aagggaatca tgtgtgaaac gcccgtcatt aaatgctgac gggtattttt caggatctgc
    15541 accaactcat tcataagctg ctcccgggct tgcgaaagcc catgtccata tttttgatgg
    15601 aggaaacaat aggcaatcgc aggcggagca aacagtggat aaaaaggcca tttactggac
    15661 ttttgtaata tcagtacaaa aatgcgatcc gcctcataac ttgcgataaa ccggaagcat
    15721 gccgcaaaac agcgtcttgt gagacgcgtc gcaaattgac ggttacatta catttgtcca
    15781 gcttttggca gatttgtcac atggcaggcc gttgccgtgc gctcttatcc tgtaaacaga
    15841 aaaccgtacc gggagagagg caatggccct gattgtggaa tttatttgtg agctacctaa
    15901 cggcgtacat gcacgtccgg caagccacgt tgaaacgctg tgtaatactt tttcatcaca
    15961 aattgagtgg cataacctgc gcactgaccg caagggcaac gccaaaagcg cccttgcgct
    16021 gattggcacc gatacgctgg cgggcgataa ctgccagtta ctgatttccg gggccgacga
    16081 acaggaagcg caccagcgtt taagccaatg gctgcgcgat gaattccccc actgcgatgc
    16141 gccgctggcg gaagttaaat ctgacgaact ggaaccactg ccggtttcac tgaccaatct
    16201 gaatccgcaa attatccgcg cccgcaccgt gtgcagcggt agtgcaggcg gcattctgac
    16261 gccgatctct tctttagatc tcaatgcgct gggtaatctt cccgcagcca aaggcgttga
    16321 cgccgagcaa tccgcactgg aaaacggcct gacgctggta ctgaaaaaca ttgagtttcg
    16381 tctgctggat agcgacggtg ccaccagcgc gattctggaa gcgcaccgat ccctggctgg
    16441 cgatacttcc ctgcgcgaac atttactggc aggcgtcagc gccggattaa gctgcgccga
    16501 agcgattgtc accagtgcga atcacttttg cgaagagttc gcccgttcca gcagcagcta
    16561 cctgcaagaa cgtgccctgg acgtacgcga cgtctgcttc cagttactcc agcaaatcta
    16621 cggtgagcaa cgcttcccgg caccgggcaa actgacgcag cccgccattt gtatggctga
    16681 tgaactgact cccagccagt tcctcgaact ggataaaaat cacctcaaag gtctgttact
    16741 gaaaagtggc ggcaccacct cacataccgt aattcttgcc cgttcgttca acattccgac
    16801 actggttggt gtagatattg atgcccttac tccgtggcag catcaaacga tttatatcga
    16861 cggcaacgct ggggcgattg tggttgagcc aggagaagcc gtagctcgtt attatcagca
    16921 agaagcccgc gtacaggacg ccctgcgtga gcaacagcgt gtctggctga cccaacaagc
    16981 ccgtaccgct gacggtatcc gcattgaaat tgccgctaac atcgctcact ccgtggaagc
    17041 gcaggccgca ttcggcaatg gtgcggaagg cgttggtttg ttccgcactg aaatgctcta
    17101 tatggatcgc accagtgcac cgggcgaaag cgagctgtac aacatttttt gtcaggcgct
    17161 ggagtctgcc aacggacgca gcattattgt gcgcactatg gacattggcg gcgacaaacc
    17221 cgttgattat ctgaacattc cggcagaagc taacccgttc ctcggttatc gcgctgtgcg
    17281 tatttatgaa gaatacgcct cgctgttcac cacacaacta cggtcgatcc tgcgcgcctc
    17341 cgctcacggc agcctgaaaa tcatgatccc gatgatctcc tcaatggaag agatcttatg
    17401 ggtgaaagaa aaactggcgg aagccaaaca gcaactgcgt aacgaacaca ttccgtttga
    17461 tgagaagatc cagcttggca ttatgctgga agtaccgtcg gtgatgttca tcatcgatca
    17521 atgctgcgaa gagattgatt tctttagtat tggtagtaat gacctgacgc aatatctgct
    17581 ggcagtggat cgcgataacg ctaaggttac tcgtcactac aacagcctga atccggcatt
    17641 cttgcgggcg ctcgattacg ccgtgcaggc ggtgcatcgc cagggcaaat ggattggtct
    17701 gtgcggtgag ctgggagcga aaggttccgt gctgccgttg ctggtcggct tagggctgga
    17761 tgaactcagc atgagcgcac catcaattcc ggcggcgaaa gcgcggatgg cgcaacttga
    17821 tagccgtgag tgccgcaagt tgctcaacca ggcaatggcc tgccgtactt cgctggaagt
    17881 ggaacacctg ctggcgcaat tccgcatgac ccaacaggac gcaccgctgg tcaccgccga
    17941 gtgcatcacg ctggaaagtg actggcgcag caaagaagaa gtgctcaaag acatgaccga
    18001 taacctgctg ctggcgggcc gctgccgcta tccgcgtaaa ctggaagccg acttatgggc
    18061 gcgcgaggcc gttttctcta ccggtctggg ctttagtttt gccattccac acagcaaatc
    18121 agaacacatt gagcaatcca ccatcagcgt ggcgcgtctg caagcgccgg tgcgctgggg
    18181 cgatgatgaa gcgcaattca tcattatgtt aaccctgaac aaacacgctg cgggcgatca
    18241 gcacatgcgc attttctcgc gcctcgctcg ccgcatcatg cacgaagaat tccgtaacgc
    18301 gctggttaac gccgcctctg ccgacgctat cgccagcctg ctgcaacatg aactggaact
    18361 gtaaaaggaa acatcatgga actgtatctg gacaccgcta acgtcgcaga agtcgaacgt
    18421 ctggcacgca tattccccat tgccggggtg acaactaacc cgagcattat cgctgccagc
    18481 aaggagtcca tatgggaagt tctgccgcgt ctgcaaaaag cgattggtga tgagggcatt
    18541 ctgtttgctc agactatgag tcgtgacgcg cagggtatgg tggaagaagc gaagcgcctg
    18601 cgcgacgcca ttccgggcat tgtggtgaaa atcccggtga cttccgaagg tctggccgcc
    18661 attaagatgc tgaaaaaaga gggtattact acgcttggca ccgcagtgta cagtgctgca
    18721 caagggttac tggccgcgct ggcaggggca aaatacgttg ctccgtatgt taaccgcgta
    18781 gatgcccagg gcggggacgg cattcgtacg gttcaggagc tgcaagcgct gttagaaatg
    18841 cacgcaccag aaagcatggt gctggcggcc agctttaaaa cgccgcgtca ggcgctggac
    18901 tgtttactgg caggatgtga atccatcacc ctgcccttag atgtagcgca acaaatgctc
    18961 aacacccctg cggtagagtc agctatagag aagttcgaac acgactggaa tgccgcattt
    19021 ggcactactc atctctaaag gagcaattat ggaccgcatt attcaatcac cgggtaaata
    19081 catccagggc gctgatgtga ttaatcgtct gggcgaatac ctgaagccgc tggcagaacg
    19141 ctggttagtg gtgggtgaca aatttgtttt aggttttgct caatccactg tcgagaaaag
    19201 ctttaaagat gctggactgg tagtagaaat tgcgccgttt ggcggtgaat gttcgcaaaa
    19261 tgagatcgac cgtctgcgtg gcatcgcgga gactgcgcag tgtggcgcaa ttctcggtat
    19321 cggtggcgga aaaaccctcg atactgccaa agcactggca catttcatgg gtgttccggt
    19381 agcgatcgca ccgactatcg cctctaccga tgcaccgtgc agcgcattgt ctgttatcta
    19441 caccgatgag ggtgagtttg accgctatct gctgttgcca aataacccga atatggtcat
    19501 tgtcgacacc aaaatcgtcg cgggcgcacc tgcacgtctg ttagcagcgg gtatcggcga
    19561 tgcgctggca acctggtttg aagcgcgtgc ttgctctcgt agcggcgcga ccaccatggc
    19621 gggcggcaag tgcacccagg cggcgctggc actggctgaa ctgtgctaca acaccctgct
    19681 ggaagaaggc gaaaaagcga tgcttgccgc cgaacagcat gtagtaaccc cggcgctgga
    19741 gcacgtgatt gaagcgaaca cctatttgag cggcgttggt tttgaaagcg gcgggctggc
    19801 ggcggctcac gcggtgcata acggcctgac cgctatcccg gaggcgcatc actattatca
    19861 cggtgaaaaa gtggcatttg gtacgctgac gcagctggtt ctggaaaacg caccggttga
    19921 ggaaatcgaa accgtagctg cacttagcca tgcggtaggt ttgccaataa ctctcgctca
    19981 actggatatt aaagaagatg tcccggcgaa aatgcgaatt gtggcagaag cggcatgtgc
    20041 agaaggtgaa accattcaca acatgcctgg cggcgcgacg ccagatcagg tttacgccgc
    20101 tctgctggta gccgaccagt acggtcagcg tttcctgcaa gagtgggaat aacctactcc
    20161 aaactcccgg cttgtccggg agtttgaacg caaaattgcc tgatgcgcta cgtttatcaa
    20221 gcctacgaaa tctctgcaat atattgaata tgcgtgcttt tgtaggccgg ataaggcgtt
    20281 cactccgcat ccggcactct gtgccagtaa tctgaaactc ccacgttgtt caggagtttt
    20341 ttatccgctt ctggacattt tctccacaga acaatcgtta gcccggaagg tcgaatctgt
    20401 gactcttatt ttcacggacg aaccagatga aagcttcact ggcgctgctc agcctgctaa
    20461 cggcatttac cagccactcc ttaaaatctc ccgccgttcc accaaccgtg gtacaaatcc
    20521 aggccaatac caaccttgcc atcgcagacg gtgccagaca gcaaatcggc agcacgctat
    20581 tttacgatcc ggcgtatatg cagcttacct atcctggcgg tgatgtcccg caagaacgcg
    20641 gggtatgttc cgatgtggtg atccgtgcat tgcgcagcca gaaggtcgat ttgcagaaac
    20701 tggtgcacga agatatggcg aagaattttg ccgagtaccc gcaaaaatgg cagttaaagc
    20761 gcccggacag caacatcgat caccgtcggg tgcctaatct ggaaacctgg tttacccgcc
    20821 acgataaaac acgccccatc agcaagaacc ccagtgacta tcaagcgggc gatattgtct
    20881 cctggcgact ggataacgga ctggcacata ttggggtggt gtcagatggc ttcgcccgcg
    20941 acggtacgcc gctggtgata cataacattg gcgcaggcgc gcaggaagaa gatgtgctgt
    21001 ttagctggcg gatggtgggg cattatcgtt attttgttaa ataagcgatc gccccacatg
    21061 aagaaaaaat tcagatgctt tttacactga cagcttcttt tttcttacgg ctcaccagcg
    21121 ccagtgccag cacaatcagc acaataccgc taccttcaac ggcccccgga ttctcaccga
    21181 gcagccacca ggaaaagagt acgccgcaaa cgggaacggc cagcgtgctt aagctggcaa
    21241 tactggcagg caagtttttc aatacaaaca accataagct ccacgccagc gccgtcgcca
    21301 gaatcgcact gtaggccagc gcccagaaca cggtgggctg ccagtcaatt tcacgttgcg
    21361 gcaccagtaa agcgaccaca ctcatcacca gcgccgcata gagcatctgc caggatgtta
    21421 acgataataa atccacgcgc ggatgacggg tgtataggcg tttagcaaca atcgcgctcg
    21481 ccccccaact gacgccggag aggattgcca gcatggcact tttcattgaa gagaaatcga
    21541 gttgccacgg ctgcaacacc aaaaataagc cgaaagcggc aatcagtatc gcgaaatatt
    21601 gcccacgtcg caggcgttca ccgagaaaca acgcggcgaa aatcaccacc cagaacggca
    21661 tggtatagct taggatcgcc accttccccg ctccgccgct gaccagcgcc cactgcgcca
    21721 ggccaaccat cccgcaggtt tgtaacaggg caatggctaa ggtgtattta aacggtgtcg
    21781 ggcgcattcc gcgaccacgt aataaaagga cgatgaataa aacgagagcg ccgaaaatgc
    21841 agcgtaaggc ggtaaagtcg aaggcaccga tgtagctggt gacttgcttc atgaaaatcc
    21901 agctataact ccagataagt gtgagcacaa ccaggccact gattgccagt gggttgctct
    21961 ttcctgcggc agacatgaga gatccagtgt gtagttttcc tgtggacact atacgccggt
    22021 aaagaaactt cagcgactgc ctgaaagaca gtcgctgaag aaggtcagat tacagcaggt
    22081 cgaaacggtc gaggttcatc actttcaccc atgccgccac gaagtcttta acaaacttct
    22141 cgtgggcatc gctgctggcg taaacttccg ctaccgcacg caggacagag ttagaaccaa
    22201 ataccagatc cgcacggctg gctgtgtatt tcacttcgcc ggtttcacgg tcacgacctt
    22261 cgaacagctc tttcgattcg tcagtcgctt tccactcgta acgcatatcc agcaagttca
    22321 cgaagaagtc attgctcaat acgccaacgc ggtcagtgaa gacgccgttt ttgctgccat
    22381 cgaagttggc acccagtaca cgcatcccgc ccaccagcgc agtcatttcc ggcgcggtca
    22441 gcgtcagttg ctgtgctttg tcgatcagca gtgactcggt ggtggaaacg tccagacgag
    22501 cgcgatagtt acggaaaccg tcagcaattg gctccagcag ctcaaacatc tcaatgtcag
    22561 tctgatcctg acgcgcatca acgcgacccg gcgcaaacgg tacatgaatg ctcaaacctg
    22621 cggcgcttgc ggctttctca acaccaacca caccagccag cacgatgata tccgccagcg
    22681 aggctttacc agactctttc tggattttct ccagaacagg cagagcacga acggctgcgg
    22741 cgttcacatc ccagtcgcgc tgcggcatta atgccagacg cgcaccgttg gcaccaccgc
    22801 gtttgtcgcc accacggaag gtagaagcag atgcccaggc caccgatacc agctcactaa
    22861 cagacagacc agaatccgca atcgcgaatt tcagatcgat aatgtcctgc tcggtcgggt
    22921 tgtagatcgg ctgcggcagc ggatcttgcc agatcagatc ttctttcggt acttccggtc
    22981 cgatgtaacg agatttcggc cccatatccc tgtgcgtcag tttgaaccag gcacgggcaa
    23041 aggcttcgtt gaacgcctgc ggatcgttga ggaaacgacg agagatcttc tcgaactcag
    23101 gatcaaaacg cagcgtcagg tcggtcacca gcatcgtcgg tttacgtttc ttcgacggat
    23161 cgaacggatc cgggataatt tccggtgcgt ctaccgcttc gaactggatt gcgccagccg
    23221 gactgcgggt ctgtacccac tcatatttga acaggttctc gaagaaatag ttgctccact
    23281 gggtcggcgt ctgggtccag acaacttcca gaccagaggt aatggcatct gcgccaacgc
    23341 cgctgccgta agtgctcgcc cagcctaaac cttgttcttc aatcggtgca gcttctggat
    23401 caggacctac atttgatgtc ggaccggcac cgtgggtttt acccagcgta tgaccacccg
    23461 caatcagcgc cacggtttct tcgtcgttca tgcccatgtt gccgaaggtc gcgcggatag
    23521 ctgctgccgc agaaagcggt tcgccgctgt ggtccgggcc ttccgggtta acgtaaatca
    23581 ggcccatctc ggttgcaccc agcggtgctt tcgccagcgc ttccggatga cggtgagtca
    23641 gccaggcttt ttcatcaccc cagttaacat ccagatccgg ttcccagacg tcttcacgac
    23701 cggcaccaaa accgaaggta cggaagccgg agttttccag cgccacgtta cctgcgagga
    23761 taaacaggtc ggcccaggag attttctgac catatttctg tttgattggc cacaacaggc
    23821 gacgcgcttt atcgaggctt acgttatccg gccaagagtt cagcggtgca aaacgttgct
    23881 gaccacgacc cgcgccgccg cgtccatcga ttgaacggta agtacccgcg ccgtgccagg
    23941 ccatacgaat aaacagaccg gcgtaactgc cccagtcggc aggccaccac ggctgagatt
    24001 ctgtcagcag ggctttcaga tcttttttca ggccgtagta atctaatttg ctgaattctt
    24061 tgcggtagtc aaagtcctca cccagtgggt tagaacgatt agaatgttgg tttaacaggt
    24121 caacacggag ttgatttggc caccagtcgc gagtggttgt gcccgccccc gcgctctggt
    24181 cgtgaccgcc ctgatggaac gggcatttgc cagtggctgt ggtgttatga atatcgtctg
    24241 acgtgctcat caatgtgctc ccctctacag tgttaccgtt acgatacaca gcgttagaga
    24301 gaagttataa ttgaattaat ccacggatgc gatagttgag atcttacata ttgttggtta
    24361 aagagatgta gatcaaattg atcgtaatta agcttttttg attatgtccg aattcggaca
    24421 aatgctttat aaaaaagggt tttaggttca tatccttcaa gtaatgaggg agaaaacaag
    24481 gctgataacc ttattttccc gccctcattt cgaggcagca ttttgtgctc tgtttaaaat
    24541 ttgtgatcac tgtgtgattt tcacaaaagc cacactattt ataaaccagg tcgaacaccc
    24601 agcgtatggc aaatcgcgta actcatttca gcgcggttaa gcgtatagaa gtggaaatct
    24661 ttcactcctt cacggcttaa aatcttcacc atatccatgg caatattcgc tcccaccagt
    24721 ttgcgggttt cggcatcgtc gtccagcccg tcaaacattt gtgccatcca cgccggaata
    24781 cgtacgttgg tcatatcggc aaatttcttc gcttgtttaa agttcgagac cggcaaaatt
    24841 cccggaataa tttccacatc aatacccgcc gatacgcagc ggtcacgaaa acgcaggtag
    24901 ctttcgacat caaagaagaa ctgagtaatc gcgcggttgg ctccggcatc gactttgcgt
    24961 ttcaggttga gcaaatccgc ctgagcgctt tttgcttccg ggtgaacctc cggatacgcc
    25021 gccacggaga tatcgaaatc tgccacttct tttaacagcg tcaccaggtc agaagcatac
    25081 atttccggct taccacttcc cggcggcaga tcgccacgca gcgccacgat atgacgaata
    25141 ccgttattcc agtagtcgcg tgcaatggtg cgcagctcgt cgggcgtcgc atcaatgcaa
    25201 gtaagatgcg gtgccgcttc cagaccagtg cgatctttaa tgcctttaat aatgctgtgc
    25261 gtacggtcgc gctcgccgga gttcgcgcca taggtcaccg atacaaactt cggtttcagg
    25321 ctgctaaggc gatcgatgga gttccacagg gtctgctcca tttcactggt acgcggcggg
    25381 aaaaactcga acgaaacgtt aatttgcccc tggacttctg ccaggctctg attcagggca
    25441 tcccgctggc tggcgtgaaa aaagctcata ccttacctca tcaatcgctt gtagttatat
    25501 gttgtgttgt gcacatctat ccgtttagac gtccagatgt aaagatgaag gaaaagccga
    25561 agggcgtcaa ccgaaaaatg accaatgatg gtgaggaaaa atcagggaag atgaaaaaac
    25621 ttcaggatgg taagaaaaag aaaatccggt atgcaaagca aaccggactg caaatataaa
    25681 ctaaccatgt acttattgct tcttgccgga ttcgtcgtaa acgcttaatc cggcctaatc
    25741 ctatgataat tcaatatatt tcattagaaa ccataaaccc gaaaacatga gtaccgggca
    25801 ttattaaatt tctgaaatta caacaactgt gccagccggt tgatatccga ctgaatcgcc
    25861 ccggcggtga cgtcgcgccc cgcgccaggc ccacggatca ccagcgggtt gtcgcgatac
    25921 cagcggcttt cgatggcaaa gacgttatcg cacggcagca gtgatgccag cggatggtct
    25981 tcacgcaccg cctctacgcc cacacgcgct ttgccgttgg catcgaaacg cgccacgtag
    26041 cgcagcacca gccccatttc gcgggccgct tccagccgtt gcaccatctg ctcgttaagt
    26101 tcatcgccat tttcaaagaa atggtcgatg ctgccgcctt cgcaatgagc aggcaccagc
    26161 gattccacac gtacctggtc cggttcaatg ttgtaacccg cttcacgcgc cagaattacc
    26221 agcttgcgca ttacgtcttt gccggagagg tcgtcacgcg gatcaggctc ggttaagccc
    26281 tgctgccacg cctgatccac cagctcggta aacggcacgc taccgtcgaa ttgcaggaac
    26341 agccaggaga gcgtgccgga gaagatcccg ctgatcgaca aaatagtatc gccgctgtcg
    26401 atcagatcgc gcacggtgtg gttgatcggc aagcccgcac cgacggtggc attgtacagc
    26461 cagtgacgcc cggttttttc gaaggcgtcg tggatctggc gatatttatt gctgtcgctg
    26521 gctcccgcca gtttgttggc gctgataacg tggaaaccgt ggctggcgaa atcaagatac
    26581 tgatcagcaa gctgctggct ggcggtaacg tccagcacca ctaaatcatc atacggatgg
    26641 gcgcgcatcc acaggaacaa cgactcttca tcctgctcaa ccgcttcatc gttgaagaag
    26701 gctaacgcgc ggctggcgtc cagcccgtca tagctcaaca ggctgcggcg gctgtccacc
    26761 acacctgcca gcacaaactc aaagccggta cgtgccgaaa gcgtgctctg ctcacgggcg
    26821 aacagttcca gccaacggga accgatattg cccttaccga acaataccag gccgatgcgt
    26881 ttttctgcgc ggaagacgga ctgatgcagc ccctgaatca ggctttcggt cgggccggtg
    26941 cgcagtactg ccaccaggct gatgccgtca tcggactgcc aggtaaattc gaccggctgg
    27001 cctttcagtt gctgccagaa gcggtggcaa tgcagcgggt tacgggtgac gcctgcaccg
    27061 accatcgcca ccagcgccag cccctgacgc aggcgcagtt cgccaggtaa tcccgcttcg
    27121 tcgaggattt tcagcgcact gtcggccact tctgaggtgt agcaaaattg cagcaactgg
    27181 cgatcgttat gtacgccaac cgccagcggg cgtacctgcg cgcgtttcag gatttggtcg
    27241 atctctttat gcgccagttt gaaatcctga ctggcgggca cctgaaactc aatcaaacag
    27301 acatcatcgt ggctggtgac aatacgcgca ccagtaccgg aggccagcac gcgttcaatg
    27361 cgcgtggaac cttgatccgg cgtgtagcta cagcgcagtt gcaggtcgat ttcgctgcca
    27421 gaaaccggct gtaaagtacg ggcgtgaaga acgggagccg ccaggcgcgc cagttcgctg
    27481 gcctcatcca gacgcagcaa cggcagcagg caggcatctt tcactttacg cgggtcggca
    27541 ctgtataccc cggcgacgtc gctccagatg gttacgcgag aaacccccgc cagcgcaccg
    27601 atttgtgtcg cggaatagtc ggaaccgtta cgccccagca gcaccgtttc accggcgttg
    27661 ttgcggctga taaatccggt caccaccaga cgtttgcccg gatgttgcac cagcagctgt
    27721 tgcagcaacg ggtaagaaag cccttcatca acctgcggtt gtgcggcgcg ttcagcgcgt
    27781 aaaaactcgc gggcatcaag ccaggccgct ggcagccctt gttgattaag taccgcagac
    27841 atcaggcgtg ccgaccacac ttcaccgtgc cccaccactt ccgcatacac tgcgtcgtta
    27901 ataccgctgt cgagcagcgc cgccaggcgc tcaaggtcgc tgacaaaagc gctaatgagg
    27961 ctatcggctt cttcagcggg tagcagaccg ctaatcagat cgcactgata gcgacgcagc
    28021 gtttgttgaa cctgatgcgc agagagacga tcggtctggc ttagtttcaa ccagttaatc
    28081 aactggttag tggtgctacc ggcggcggaa accaccatca tatcgtcagg ctgagagtac
    28141 tccgccataa tgcccgcgac acgcaaataa cacttcacat cagccagact actgccacca
    28201 aatttatgca gctgacgacc tttcgcccct gcctgcgcaa tcacactcat ttttacccct
    28261 tgtttgcagc ccggaagcca ttttccaggt cggcaattaa atcttcgcca tcttcaatac
    28321 cggtggagat acgcagcagc gtctcggaga tcccggcggc agcacgcgct tctggtgcca
    28381 tgcctgcatg tgtcatggtt gcggcgtgag agattaaact ttccactccc cctaatgatt
    28441 ccgccagcgt aaacaacgac agcccgctca ggaaacgacg cagcgtctgc tcatcgccat
    28501 ccagttcaaa actcaacatt gcgccaaagc ctttttgctg acgcgcggca atttcatgcc
    28561 cctgattttc cggcagcgac gggtgataca gttttttcac caacggctgg gtttgcagat
    28621 atttcacaat cgcctgcgcg ttacgctggg ccaactccat gcgcggcacc agcgttcgca
    28681 acccgcgtag cagcagatag ctgtcaaacg cgccgcccgt cacgccaata ttgtttgccc
    28741 accaggcaag ttcagtgaca acgtccgggt ctttagcaat caccacgccg gccaccacgt
    28801 ctgagtgacc gttcagatat ttcgtgcatg aatgcaacac cagatcggca cctaatgcca
    28861 gcggattttg taatgccggg cttaagaagg tgttatccac cacgctcacc gccccgactt
    28921 cccttgccag atggcagatt ttcgcaatat ccacgacgcg taacaatgga ttacttgggc
    28981 tttctaccag taccagtttg ggtttttctg ccagcgctgc ccgtaatgcc tgttcatcgc
    29041 cttgatcaac aaacaacacg cgatagcaac cacgtttcgc caggctgtcg aacaggcgat
    29101 agctaccgcc gtagcagtcg tgcggcgcta ccagcagatc gccaggtttc aaaaagacgg
    29161 tcgttaccag gtgaatcgcg gacatgccgg tattagtaag tactgcacca gcaccgcctt
    29221 ccagttctgc cagtgctcgc tgaaccacat cgcgcgttgg gttgccacga cgcgagtaat
    29281 catgcgcgcg cggttcatta aatccggtaa agttataggt actggaaaga tggatgggtg
    29341 ggacaacgca accatactgt tcgtcgtcat ttaacccgct acgcactgcg atggtggcct
    29401 gtttacgcgt catgtgatga tgttccctgg gctttattgg tgaaatgtca ggcaccagag
    29461 taaacattgt ggtaatggac gtcaatacat ctggacatct aaacttcttt gcgtatagat
    29521 tgagcaaatc ccaaatagcc gttaaaatta tatgcattat cacgccagca ggtggattac
    29581 ccgatgtaac ggtagcgcct gtacggtaaa ctatgcgggt ttacggccag tgcccacatt
    29641 aactgtgtgg tctggtctca atttattgac gaagaggatt aagtatctca tggctgaatg
    29701 gagcggcgaa tatatcagcc catacgctga gcacggcaag aagagtgaac aagtcaaaaa
    29761 gattacggtt tccattcctc ttaaggtgtt aaaaatcctc accgatgaac gcacgcgtcg
    29821 tcaggtgaac aacctgcgtc acgctaccaa cagcgagctg ctgtgcgaag cgtttctgca
    29881 tgcctttacc gggcaacctt tgccggatga tgccgatctg cgtaaagagc gcagcgacga
    29941 aatcccggaa gcggcaaaag agatcatgcg tgagatgggg attaacccgg agacgtggga
    30001 atactaaagc ggaaaagagc ggcgcggagt ggaaccgcct gatgcgctac gcttatcagg
    30061 cctacgtcat attgcaattt attgaatttg cacgaacttg taggccggat aaggcgttca
    30121 cgccgcatcc ggcataaaca acgagcacgt tgtctgcgac ccaccgcttt ttatacatgg
    30181 acgtttaact atgaaaaaca ggctgctgat cctcagcctg ctggtttctg tacctgcctt
    30241 tgcctggcag ccgcaaactg gcgacatcat ctttcaaatc tctcgctcat cgcaaagtaa
    30301 agcgatccaa ctggcaaccc attccgatta cagccacacc ggtatgctgg tgatacgcaa
    30361 caaaaagccc tacgtttttg aagcggtcgg cccggtgaaa tacaccccgc tcaagcagtg
    30421 gatcgcccat ggtgaaaagg gcaaatacgt cgttcgccgc gttgaaggcg gactgagtgt
    30481 tgaacaacag caaaaactgg cgcaaacggc aaaacgttat ctcggtaaac cgtatgattt
    30541 cagcttctca tggagcgacg atcgccagta ctgttcggaa gtggtgtgga aggtttacca
    30601 gaacgcgctg ggaatgcgcg tgggcgaaca gcagaagttg aaagagtttg atctcagcaa
    30661 cccgctggtc caggcgaagc tcaaagaacg ctacggtaaa aatattccgc ttgaggaaac
    30721 ggtagtttca ccacaagccg ttttcgacgc gccacaactc accacggttg ccaaagagtg
    30781 gccgctgttt tcgtggtaat ggatgatgaa aaaaataatt cccgccgtag tctgtttgtt
    30841 gctttgtagt tgcacgacct ttccgcaagc tgtcgctccg gtaaacggtg atgcctccaa
    30901 ttagttgaat taaggatata atactcgctt ttaaggttgt ttcatggtca cagttaacct
    30961 tcattgcccc cgttgccagt ccgttcaggt ttaccgtcac gggcagaacc caaaaagaca
    31021 tgatcgattc cgcggccgtg actgtcaccg ggtatttcag ctcagttact gttacgagcc
    31081 ccgtaaaccg ggagttaaag accaaatcgc tgaaatgact ttcaacggtg ccggggttcg
    31141 cgatactgcc cgtacgctga aaatcggcat taatacagtc atccgaactt taaaaaactc
    31201 tcgccacgaa gaataacgtc atccccggtc gctcatgctg atgtggcact catctgcaaa
    31261 cttagtcagc aatggagctt tgtcggcagt aaagcgcgac agcactggct ctggtacgtg
    31321 tacaacacga aaactggtgg tgttctggcc tacacttttg gcccccggac tgacgaaacc
    31381 tgtcgtgaat tgcgggcgct gcttaccctt ttaccatcgg catgttgacc actgacgaat
    31441 ggggtagtta cgctagagag ttaccgaagg agaagcatct gactggtaag atttttactc
    31501 agcacattga gcgtaacaat ctgaccctga gaacccgaat caaacgtctg gcccgcagaa
    31561 ccatctgttt ctcgcgttct gtagaacttc atgaaaaagt catcggtgct tttatcgaaa
    31621 atatatgttc tactaattgg agtcatcacc cttctgataa gctttccttg gatgattacc
    31681 agactgaata cacagccaaa gatttttacc tcattctgct cttttttctc aaatgacata
    31741 tatt
//
LOCUS       DAZGVQ010000045        31333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000045 DAZGVQ010000000
VERSION     DAZGVQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.045"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..567
                     /locus_tag="ACHLAG_001983"
     CDS             <1..567
                     /locus_tag="ACHLAG_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFP0655223.1"
                     /translation="IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKE
                     TQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESE
                     VYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHP
                     IAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            797..1180
                     /gene="secE"
                     /locus_tag="ACHLAG_001984"
     CDS             797..1180
                     /gene="secE"
                     /locus_tag="ACHLAG_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFP0655224.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1182..1727
                     /gene="nusG"
                     /locus_tag="ACHLAG_001985"
     CDS             1182..1727
                     /gene="nusG"
                     /locus_tag="ACHLAG_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFP0655225.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATTLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1886..2314
                     /gene="rplK"
                     /locus_tag="ACHLAG_001986"
     CDS             1886..2314
                     /gene="rplK"
                     /locus_tag="ACHLAG_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFP0655226.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2318..3022
                     /gene="rplA"
                     /locus_tag="ACHLAG_001987"
     CDS             2318..3022
                     /gene="rplA"
                     /locus_tag="ACHLAG_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFP0655227.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3435..3932
                     /gene="rplJ"
                     /locus_tag="ACHLAG_001988"
     CDS             3435..3932
                     /gene="rplJ"
                     /locus_tag="ACHLAG_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFP0655228.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3999..4364
                     /gene="rplL"
                     /locus_tag="ACHLAG_001989"
     CDS             3999..4364
                     /gene="rplL"
                     /locus_tag="ACHLAG_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFP0655229.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4684..8712
                     /gene="rpoB"
                     /locus_tag="ACHLAG_001990"
     CDS             4684..8712
                     /gene="rpoB"
                     /locus_tag="ACHLAG_001990"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP0655230.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8789..13012
                     /gene="rpoC"
                     /locus_tag="ACHLAG_001991"
     CDS             8789..13012
                     /gene="rpoC"
                     /locus_tag="ACHLAG_001991"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFP0655231.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            13225..13764
                     /gene="yjaZ"
                     /locus_tag="ACHLAG_001992"
     CDS             13225..13764
                     /gene="yjaZ"
                     /locus_tag="ACHLAG_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase growth adaptation protein"
                     /protein_id="HFP0655232.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQSSK"
     gene            complement(14174..15307)
                     /gene="thiH"
                     /locus_tag="ACHLAG_001993"
     CDS             complement(14174..15307)
                     /gene="thiH"
                     /locus_tag="ACHLAG_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFP0655233.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(15304..16074)
                     /gene="thiG"
                     /locus_tag="ACHLAG_001994"
     CDS             complement(15304..16074)
                     /gene="thiG"
                     /locus_tag="ACHLAG_001994"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFP0655234.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(16076..16276)
                     /gene="thiS"
                     /locus_tag="ACHLAG_001995"
     CDS             complement(16076..16276)
                     /gene="thiS"
                     /locus_tag="ACHLAG_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFP0655235.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(16260..17015)
                     /gene="thiF"
                     /locus_tag="ACHLAG_001996"
     CDS             complement(16260..17015)
                     /gene="thiF"
                     /locus_tag="ACHLAG_001996"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFP0655236.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(17008..17643)
                     /gene="thiE"
                     /locus_tag="ACHLAG_001997"
     CDS             complement(17008..17643)
                     /gene="thiE"
                     /locus_tag="ACHLAG_001997"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFP0655237.1"
                     /translation="MYQPDFPPVPFRSGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17643..19538)
                     /gene="thiC"
                     /locus_tag="ACHLAG_001998"
     CDS             complement(17643..19538)
                     /gene="thiC"
                     /locus_tag="ACHLAG_001998"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFP0655238.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQVSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19619..19719)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19771..20247)
                     /gene="rsd"
                     /locus_tag="ACHLAG_001999"
     CDS             complement(19771..20247)
                     /gene="rsd"
                     /locus_tag="ACHLAG_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFP0655239.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20342..21115
                     /gene="nudC"
                     /locus_tag="ACHLAG_002000"
     CDS             20342..21115
                     /gene="nudC"
                     /locus_tag="ACHLAG_002000"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFP0655240.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            21155..22219
                     /gene="hemE"
                     /locus_tag="ACHLAG_002001"
     CDS             21155..22219
                     /gene="hemE"
                     /locus_tag="ACHLAG_002001"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFP0655241.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLVRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            22229..22900
                     /gene="nfi"
                     /locus_tag="ACHLAG_002002"
     CDS             22229..22900
                     /gene="nfi"
                     /locus_tag="ACHLAG_002002"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFP0655242.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22943..23533
                     /gene="yjaG"
                     /locus_tag="ACHLAG_002003"
     CDS             22943..23533
                     /gene="yjaG"
                     /locus_tag="ACHLAG_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFP0655243.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23720..23992
                     /gene="hupA"
                     /locus_tag="ACHLAG_002004"
     CDS             23720..23992
                     /gene="hupA"
                     /locus_tag="ACHLAG_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFP0655244.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            24005..24700
                     /gene="yjaH"
                     /locus_tag="ACHLAG_002005"
     CDS             24005..24700
                     /gene="yjaH"
                     /locus_tag="ACHLAG_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFP0655245.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRAAQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24702..25127)
                     /gene="zraP"
                     /locus_tag="ACHLAG_002006"
     CDS             complement(24702..25127)
                     /gene="zraP"
                     /locus_tag="ACHLAG_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFP0655246.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYTQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            complement(25130..25258)
                     /locus_tag="ACHLAG_002007"
     CDS             complement(25130..25258)
                     /locus_tag="ACHLAG_002007"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655247.1"
                     /translation="MTRYPQQASHFYSFIMPDYPSYQRFIVGTEDAIPEVRQPLED"
     gene            25365..26741
                     /gene="zraS"
                     /locus_tag="ACHLAG_002008"
     CDS             25365..26741
                     /gene="zraS"
                     /locus_tag="ACHLAG_002008"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFP0655248.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVGQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26738..28063
                     /gene="zraR"
                     /locus_tag="ACHLAG_002009"
     CDS             26738..28063
                     /gene="zraR"
                     /locus_tag="ACHLAG_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFP0655249.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VRERVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDTLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLASHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERSVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQLLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(28060..29349)
                     /gene="purD"
                     /locus_tag="ACHLAG_002010"
     CDS             complement(28060..29349)
                     /gene="purD"
                     /locus_tag="ACHLAG_002010"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFP0655250.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTNIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29361..30950)
                     /gene="purH"
                     /locus_tag="ACHLAG_002011"
     CDS             complement(29361..30950)
                     /gene="purH"
                     /locus_tag="ACHLAG_002011"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFP0655251.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7380 a         8116 c         8580 g         7257 t
ORIGIN      
        1 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
       61 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      121 ggtatcaaag agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      181 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      241 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      301 tctgaagtgt acattctgtc caaagatgaa ggcggtcgtc atactccgtt cttcaaaggc
      361 taccgtccgc agttctactt ccgtactact gacgtgactg gtaccatcga actgccggaa
      421 ggcgtagaga tggtaatgcc gggcgacaac atcaaaatgg ttgttaccct gatccacccg
      481 atcgcgatgg acgacggtct gcgtttcgca atccgtgaag gcggccgtac cgttggcgcg
      541 ggcgttgtag caaaagttct gagctaattg ccgataacat ttgacgcaat gcgcactaaa
      601 agggcatcat ttgatgccct ttttgcacgc tttcgtacca gaacctggct catcagtgat
      661 tttctttgtc ataatcattg ctgagacagg ctctgttgag ggcgtataat ccgaaaagct
      721 aatacgcgtt tcgatttggt ttgcctcgcg atcgcggggt gaaaatgttt gtagaaaact
      781 tctgacaggt tggtttatga gtgcgaatac cgaagctcaa ggaagcgggc gcggcctgga
      841 agcgatgaag tgggtcgttg tggtggcatt gctcctggtg gcgattgtcg gcaactacct
      901 ttatcgcgac attatgctgc cgctgcgtgc gctggccgta gtaattctga ttgctgcagc
      961 gggtggtgtc gcgctgttaa cgacaaaagg taaagctacc gttgcttttg cccgtgaagc
     1021 gcgtaccgaa gtccgtaagg tcatttggcc gactcgccag gaaacattgc acaccacgct
     1081 gattgtggct gcggttaccg cagtaatgtc actgatcctg tggggactgg atggtattct
     1141 ggttcgcctg gtatccttta tcactggcct gaggttctga gatgtctgaa gctcctaaaa
     1201 agcgctggta cgtcgttcag gcgttttccg gttttgaagg ccgcgtagca acgacgctgc
     1261 gtgagcatat caaattacac aacatggaag atttgtttgg tgaagtcatg gtaccaaccg
     1321 aagaagtggt tgaaatccgt ggcggtcagc gtcgcaaaag cgaacgtaaa ttcttccctg
     1381 gctacgtcct cgttcagatg gtgatgaacg acgcgagctg gcacctggtg cgcagcgtac
     1441 cgcgtgtgat gggcttcatc ggcggtactt ccgatcgtcc tgcgccaatc agcgataaag
     1501 aagtcgatgc gattatgaac cgcctgcagc aggttggtga taagccgcgt ccgaaaacgc
     1561 tgtttgaacc gggtgaaatg gtccgtgtta atgatggtcc gttcgctgac ttcaacggtg
     1621 ttgttgaaga agtggattac gagaaatctc gtctgaaagt gtctgtttct atcttcggtc
     1681 gtgcgacccc ggtagagctg gacttcagcc aggttgaaaa agcctaaccc agcgatcaaa
     1741 aaagcggcga tttaatcgtt gcacaaggcg tgagattgga atacaatttc gcgccttttg
     1801 tttttatggg ccttgcccgt aaaacgattt tttatatcac ggggagcctc tcagaggcgt
     1861 tattacccaa cttgaggaat ttataatggc taagaaagta caagcctatg tcaagctgca
     1921 ggttgcagct ggtatggcta acccgagtcc gccagtaggt ccggctctgg gtcagcaggg
     1981 cgtaaacatc atggaattct gcaaagcgtt caacgcaaaa actgattcca tcgaaaaagg
     2041 tctgccgatt ccggtagtaa tcaccgttta cgctgaccgt tctttcactt tcgttaccaa
     2101 gactccgccg gcagcagttc tgctgaaaaa agcggctggt atcaagtctg gttccggtaa
     2161 gccgaacaaa gacaaagtgg gtaaaatttc ccgcgctcag ctgcaggaaa tcgcgcagac
     2221 caaagctgcc gacatgactg gtgccgacat tgaagcgatg actcgctcca tcgaaggtac
     2281 tgcacgttcc atgggcctgg tagtggagga ctaagaaatg gctaaactga ccaagcgcat
     2341 gcgtgttatc cgcgagaaag ttgatgcaac caaacagtac gacatcaacg aagctatcgc
     2401 actgctgaaa gagctggcga ctgctaaatt cgtagaaagc gtggacgtag ctgttaacct
     2461 cggcatcgac gctcgtaaat ctgaccagaa cgtacgtggt gcaactgtac tgccgcacgg
     2521 tactggccgt tccgttcgcg tagccgtatt tacccaaggt gcaaacgctg aagctgctaa
     2581 agctgcaggc gcagaactgg taggtatgga agatctggct gaccagatca agaaaggcga
     2641 aatgaacttt gacgttgtta ttgcttctcc ggatgcaatg cgcgttgttg gccagctggg
     2701 ccaggttctg ggtccgcgcg gcctgatgcc aaacccgaaa gtgggtactg taacaccgaa
     2761 cgttgctgaa gcggttaaaa acgctaaagc tggccaggtt cgttaccgta acgacaaaaa
     2821 cggcatcatc cacaccacca tcggtaaagt ggactttgac gctgacaaac tgaaagaaaa
     2881 cctggaagct ctgctggttg cgctgaaaaa agcaaaaccg actcaggcga aaggcgtgta
     2941 catcaagaaa gttagcatct ccaccaccat gggtgcaggt gttgcagttg accaggctgg
     3001 cctgagcgct tctgtaaact aatgccttta cgtgggcggt gattttgtct acaatcttac
     3061 ccccacgtat aatgcttaat gcagacgtat atccgagata ttcgggttgt ggcaaggcgg
     3121 caactgagtg agtcgccagg agcatagcta actatgtgac tggtgcgaat gaaggaagcc
     3181 aacgccgcca caagttgaat agcgacggat agaaaagatt tgttcgttgg agcctggcct
     3241 atccaggcct ccgtcgaaga ccgcaggagt ttcgcaagaa acttaatccc ctgcgtagac
     3301 ggtgacagaa cgctaagatt attcttttat attctggctt gtttctgctc accgtaatta
     3361 agacgctctc tccgtttgga ggagtgaagt gagttccaga gattttctct ggcaaacatc
     3421 caggagcaaa gctaatggct ttaaatcttc aagacaaaca agcgattgtt gctgaagtca
     3481 gcgaagtagc caaaggcgcg ctgtctgcag tagttgcgga ttcccgtggc gtaactgtag
     3541 ataaaatgac tgaactgcgt aaagcaggtc gcgaagctgg cgtatacatg cgtgttgttc
     3601 gtaacaccct gctgcgccgt gctgttgaag gtactccgtt cgagtgcctg aaagacgcgt
     3661 ttgttggtcc gaccctgatt gcatactcta tggaacaccc gggcgctgct gctcgtctgt
     3721 tcaaagagtt cgcgaaagcg aatgcaaaat ttgaggtcaa agccgctgcc tttgaaggtg
     3781 agctgatccc ggcgtctcag atcgaccgcc tggcaactct gccgacctac gaagaagcaa
     3841 ttgcacgcct gatggcaacc atgaaagaag cttcggctgg caaactggtt cgtactctgg
     3901 ctgctgtacg cgatgcgaaa gaagctgctt aatcgcagtt atctttttaa cgcattcgct
     3961 tacgtataaa cttattctga tattcaggaa caatttaaat gtctatcact aaagatcaaa
     4021 tcattgaagc agttgcagct atgtctgtaa tggacgttgt agaactgatc tctgcaatgg
     4081 aagaaaaatt cggtgtttcc gctgctgctg ctgtagctgt agctgctggc ccggttgaag
     4141 ctgctgaaga aaaaactgaa ttcgacgtaa ttctgaaagc tgctggcgct aacaaagttg
     4201 ctgttatcaa agcagtacgt ggcgcaactg gcctgggtct gaaagaagct aaagacctgg
     4261 tagaatctgc accggctgca ctgaaagaag gcgtgagcaa agacgacgca gaagcactga
     4321 aaaaagctct ggaagaagct ggcgctgaag ttgaagttaa ataagccaac ccttccggtt
     4381 gcagcctgag aaatcaggct gatggctggt gactttttag tcaccagcct ttttgcgctg
     4441 taaggcgcca gtagcgtttc acactgtttg actactgctg tgcctttcaa tgcttgtttc
     4501 tatcgacgac ttaatatact gcgacaggac gtccgttctg tgtaaatcgc aatgaaatgg
     4561 tttaagcgtg atagcaacag gcattgcgga aagtgttcca ttttccggtc aacaaaatag
     4621 tgttgcacaa actgtccgct caatggacag atgggtcgac ttgtcagcga gctgaggaac
     4681 cctatggttt actcctatac cgagaaaaaa cgtattcgta aggattttgg taaacgtcca
     4741 caagttctgg atgtacctta tctcctttct atccagcttg actcgtttca gaaatttatc
     4801 gagcaagatc ctgaagggca gtatggtctg gaagctgctt tccgttccgt attcccgatt
     4861 cagagctaca gcggtaattc cgagctgcaa tacgtcagct accgccttgg cgaaccggtg
     4921 tttgacgtcc aggaatgtca aatccgtggc gtgacctatt ccgcaccgct gcgcgttaaa
     4981 ctgcgtctgg tgatctatga gcgcgaagcg ccggaaggca ccgtaaaaga cattaaagaa
     5041 caagaagtct acatgggcga aattccgctc atgacagaca acggtacctt tgttatcaac
     5101 ggtactgagc gtgttatcgt ttcccagctg caccgtagtc cgggcgtctt ctttgactcc
     5161 gacaaaggta aaacccactc ttcgggtaaa gtgctgtata acgcgcgtat catcccttac
     5221 cgtggttcct ggctggactt cgaattcgat ccgaaggaca acctgttcgt acgtatcgac
     5281 cgtcgccgta aactgcctgc gaccatcatt ctgcgcgccc tgaactacac cacagagcag
     5341 atcctcgacc tgttctttga aaaagttatc tttgaaatcc gtgataacaa gctgcagatg
     5401 gaactggtgc cggaacgcct gcgtggtgaa accgcatctt ttgacatcga agctaacggt
     5461 aaagtgtacg tagaaaaagg ccgccgtatc actgcgcgcc acattcgcca gctggaaaaa
     5521 gacgacgtca aactgatcga agtcccggtt gagtacatcg caggtaaagt ggttgctaaa
     5581 gactatattg atgagtctac cggcgagctg atctgcgcag cgaacatgga gctgagcctg
     5641 gatctgctgg ctaagctgag ccagtctggt cacaagcgta tcgaaacgct gttcaccaac
     5701 gatctggatc acggcccata tatctctgaa accttacgtg tcgacccaac taacgaccgt
     5761 ctgagcgcac tggtagaaat ctaccgcatg atgcgccctg gcgagccgcc gactcgtgaa
     5821 gcagctgaaa gcctgttcga gaacctgttc ttctccgaag accgttatga cttgtctgcg
     5881 gttggtcgta tgaagttcaa ccgttctctg ctgcgcgaag aaatcgaagg ttccggtatc
     5941 ctgagcaaag acgacatcat tgatgttatg aaaaagctca tcgatatccg taacggtaaa
     6001 ggcgaagtcg atgatatcga ccacctcggc aaccgtcgta tccgttccgt tggcgaaatg
     6061 gcggaaaacc agttccgcgt tggcctggta cgtgtagagc gtgcggtgaa agagcgtctg
     6121 tctctgggcg atctggatac cctgatgcct caggatatga tcaacgccaa gccgatttcc
     6181 gcagcagtga aagagttctt cggttccagc cagctgtctc agtttatgga ccagaacaac
     6241 ccgctgtctg agattacgca caaacgtcgt atctccgcac tcggcccagg cggtctgacc
     6301 cgtgaacgtg caggcttcga agttcgagac gtacacccga ctcactacgg tcgcgtatgt
     6361 ccaatcgaaa cccctgaagg tccgaacatc ggtctgatca actctctgtc cgtgtacgca
     6421 cagactaacg aatacggctt ccttgagact ccgtatcgta aagtgaccga cggtgttgta
     6481 actgacgaaa ttcactacct gtctgctatc gaagaaggca actacgttat cgcccaggcg
     6541 aactccaacc tggatgaaga aggccacttc gtagaagacc tggtaacttg ccgtagcaaa
     6601 ggcgaatcca gcttgttcag ccgcgaccag gttgactaca tggacgtatc cacccagcag
     6661 gtggtatccg tcggtgcgtc cctgatcccg ttcctggaac acgatgacgc caaccgtgca
     6721 ttgatgggtg cgaacatgca acgtcaggcc gttccgactc tgcgcgctga taagccgctg
     6781 gttggtactg gtatggaacg tgctgttgcc gttgactccg gtgtaactgc ggtagctaaa
     6841 cgtggtggtg tcgttcagta cgtggatgct tcccgtatcg ttatcaaagt taacgaagac
     6901 gagatgtatc cgggtgaagc aggtatcgac atctacaacc tgaccaaata cacccgttct
     6961 aaccagaaca cctgtatcaa ccagatgccg tgtgtgtctc tgggtgaacc ggttgaacgt
     7021 ggcgacgtgc tggcagacgg tccgtccacc gacctcggtg aactggcgct tggtcagaac
     7081 atgcgcgtag cgttcatgcc gtggaatggt tacaacttcg aagactccat cctcgtatcc
     7141 gagcgtgttg ttcaggaaga ccgtttcacc accatccaca ttcaggaact ggcgtgtgtg
     7201 tcccgtgaca ccaagctggg gccggaagag atcaccgctg acatcccgaa cgtgggtgaa
     7261 gctgcgctct ccaaactgga tgaatccggt atcgtttaca ttggtgcgga agtgaccggt
     7321 ggcgacattc tggttggtaa ggtaacgccg aaaggtgaaa ctcagctgac cccagaagaa
     7381 aaactgctgc gtgcgatctt cggtgagaaa gcctctgacg ttaaagactc ttctctgcgc
     7441 gtaccaaacg gtgtatccgg tacggttatc gacgttcagg tctttactcg cgatggcgta
     7501 gaaaaagaca aacgtgcgct ggaaatcgaa gaaatgcagc tcaaacaggc gaagaaagac
     7561 ctgtctgaag aactgcagat cctcgaagcg ggtctgttca gccgtatccg tgctgtgctg
     7621 gtagccggtg gcgttgaagc tgagaagctc gacaaactgc cgcgcgatcg ctggctggag
     7681 ctgggcctga cagacgaaga gaaacaaaat cagctggaac agctggctga gcagtatgac
     7741 gaactgaaac acgagttcga gaagaaactc gaagcgaaac gccgcaaaat cacccagggc
     7801 gacgatctgg caccgggcgt gctgaagatt gttaaggtat atctggcggt taaacgccgt
     7861 atccagcctg gtgacaagat ggcaggtcgt cacggtaaca agggtgtaat ttctaagatc
     7921 aacccgatcg aagatatgcc ttacgatgaa aacggtacgc cggtagacat cgtactgaac
     7981 ccgctgggcg taccgtctcg tatgaacatc ggtcagatcc tcgaaaccca cctgggtatg
     8041 gctgcgaaag gtatcggcga caagatcaac gccatgctga aacagcagca agaagtcgcg
     8101 aaactgcgcg aattcatcca gcgtgcgtac gatctgggcg ctgacgttcg tcagaaagtt
     8161 gacctgagta ccttcagcga tgaagaagtt atgcgtctgg ctgaaaacct gcgcaaaggt
     8221 atgccaatcg caacgccggt gttcgacggt gcgaaagaag cagaaattaa agagctgctg
     8281 aaacttggtg acctgccgac ttccggtcag atccgcctgt acgatggtcg cactggtgaa
     8341 cagttcgagc gtccggtaac cgttggttac atgtacatgc tgaaactgaa ccacctggtc
     8401 gacgacaaga tgcacgcgcg ttccaccggt tcttacagcc tggttactca gcagccgctg
     8461 ggtggtaagg cacagttcgg tggtcagcgt ttcggggaga tggaagtgtg ggcgctggaa
     8521 gcatacggcg cagcatacac cctgcaggaa atgctcaccg ttaagtctga tgacgtgaac
     8581 ggtcgtacca agatgtataa aaacatcgtg gacggcaacc atcagatgga gccgggcatg
     8641 ccagaatcct tcaacgtatt gttgaaagag attcgttcgc tgggtatcaa catcgaactg
     8701 gaagacgagt aattctcgct caaacaggtc actgctgtcg ggttaaaacc cggcagcgga
     8761 ttgtgctaac tccgacggga gcaaatccgt gaaagattta ttaaagtttc tgaaagcgca
     8821 gactaaaacc gaagagtttg atgcgatcaa aattgctctg gcttcgccag acatgatccg
     8881 ttcatggtct ttcggtgaag ttaaaaagcc ggaaaccatc aactaccgta cgttcaaacc
     8941 agaacgtgac ggccttttct gcgcccgtat ctttgggccg gtaaaagatt acgagtgcct
     9001 gtgcggtaag tacaagcgcc tgaaacaccg tggcgtcatc tgtgagaagt gcggcgttga
     9061 agtgacccag actaaagtac gccgtgagcg tatgggccac atcgaactgg cttccccgac
     9121 tgcgcacatc tggttcctga aatcgctgcc gtcccgtatc ggtctgctgc tcgatatgcc
     9181 gctgcgcgat atcgaacgcg tactgtactt tgaatcctat gtggttatcg aaggcggtat
     9241 gaccaacctg gaacgtcagc agatcctgac tgaagagcag tatctggacg cgctggaaga
     9301 gttcggtgac gaattcgacg cgaagatggg ggcggaagca atccaggctc tgctgaagag
     9361 catggatctg gagcaagagt gcgaacagct gcgtgaagag ctgaacgaaa ccaactccga
     9421 aaccaagcgt aaaaagctga ccaagcgtat caaactgctg gaagcgttcg ttcagtctgg
     9481 taacaaacca gagtggatga tcctgaccgt tctgccggta ctgccgccag atctgcgtcc
     9541 gctggttccg ctggatggtg gtcgtttcgc gacttctgac ctgaacgatc tgtatcgtcg
     9601 cgtcattaac cgtaacaacc gtctgaaacg tctgctggat ctggctgcgc cggacatcat
     9661 cgtacgtaac gaaaaacgta tgctgcagga agcggtagac gccctgctgg ataacggtcg
     9721 tcgcggtcgt gcgatcaccg gttctaacaa gcgtcctctg aaatctttgg ccgacatgat
     9781 caaaggtaaa cagggtcgtt tccgtcagaa cctgctcggt aagcgtgttg actactccgg
     9841 tcgttctgta atcaccgtag gtccatacct gcgtctgcat cagtgcggtc tgccgaagaa
     9901 aatggcgctg gagctgttca aaccgttcat ctacggcaag ctggaactgc gtggtcttgc
     9961 taccaccatt aaagctgcga agaaaatggt tgagcgcgaa gaagctgtcg tttgggatat
    10021 cctggacgaa gttatccgcg aacacccggt actgctgaac cgtgcaccga ctctgcaccg
    10081 tctgggtatc caggcatttg aaccggtact gatcgaaggt aaagctatcc agctgcaccc
    10141 gctggtttgt gcggcatata acgccgactt cgatggtgac cagatggctg ttcacgtacc
    10201 gctgacgctg gaagcccagc tggaagcgcg tgcgctgatg atgtctacca acaacatcct
    10261 gtccccggcg aacggcgaac caatcatcgt tccgtctcag gacgttgttc tgggtctgta
    10321 ctatatgacc cgtgactgtg ttaacgccaa aggcgaaggc atggtgctga ctggcccgaa
    10381 agaagcagaa cgtctgtatc gctctggtct ggcttctctg catgcgcgcg ttaaagtgcg
    10441 tatcaccgag tatgaaaaag atgcaaacgg tgaattagta gcgaaaacca gcctgaaaga
    10501 cacgactgtt ggccgtgcca ttctgtggat gattgtaccg aaaggtctgc cttactccat
    10561 cgtcaaccag gcgctgggta aaaaagcaat ctccaaaatg ctgaacacct gctaccgcat
    10621 tctcggtctg aaaccgaccg ttatttttgc ggaccagatc atgtacaccg gcttcgccta
    10681 tgcagcgcgt tctggtgcat ctgttggtat cgatgacatg gtcatcccgg agaagaaaca
    10741 cgaaatcatc tccgaggcag aagcagaagt tgctgaaatt caggagcagt tccagtctgg
    10801 tctggtaact gcgggcgaac gctacaacaa agttatcgat atctgggctg cggcgaacga
    10861 tcgtgtatcc aaagcgatga tggataacct gcaaactgaa accgtgatta accgtgacgg
    10921 tcaggaagag aagcaggttt ccttcaacag catctacatg atggccgact ccggtgcgcg
    10981 tggttctgcg gcacagattc gtcagcttgc tggtatgcgt ggtctgatgg cgaagccgga
    11041 tggctccatc atcgaaacgc caatcaccgc gaacttccgt gaaggtctga acgtactcca
    11101 gtacttcatc tccacccacg gtgctcgtaa aggtctggcg gataccgcac tgaaaactgc
    11161 gaactccggt tacctgactc gtcgtctggt tgacgtggcg caggacctgg tggttaccga
    11221 agacgattgt ggtacccatg aaggtatcat gatgactccg gttatcgagg gtggtgacgt
    11281 taaagagccg ctgcgcgatc gcgtactggg tcgtgtaact gctgaagacg ttctgaagcc
    11341 gggtactgct gatatcctcg ttccgcgcaa cacgctgctg cacgaacagt ggtgtgacct
    11401 gctggaagag aactctgtcg acgcggttaa agtacgttct gttgtatctt gtgacaccga
    11461 ctttggtgta tgtgcgcact gctacggtcg tgacctggcg cgtggccaca tcatcaacaa
    11521 gggtgaagca atcggtgtta tcgcggcaca gtccatcggt gaaccgggta cacagctgac
    11581 catgcgtacg ttccacatcg gtggtgcggc atctcgtgcg gctgctgaat ccagcatcca
    11641 ggtgaaaaac aaaggtagca tcaagctcag caacgtgaag tcggttgtga actccagcgg
    11701 taaactggtt atcacttccc gtaatactga actgaaactg atcgacgaat tcggtcgtac
    11761 caaagaaagc tacaaagtac cttacggtgc ggtactggcg aaaggcgatg gcgaacaggt
    11821 tgctggcggc gaaaccgttg caaactggga cccgcacacc atgccggtta tcaccgaagt
    11881 aagcggtttt gtacgcttta ctgacatgat cgacggtcag accattactc gtcagacaga
    11941 cgaattgacc ggtctgtctt cgctggtggt tctggattcc gcagaacgta ccgcaggtgg
    12001 taaagatctg cgtccggcac tgaaaatcgt tgatgctcag ggtaacgacg ttctgatccc
    12061 aggtaccgat atgcctgcgc agtacttcct gccgggtaaa gcgattgttc agctggaaga
    12121 tggcgtacag atcagctctg gtgacaccct ggcgcgtatt ccgcaggaat ccggcggtac
    12181 caaggacatc accggtggtc tgccgcgcgt tgcggacctg ttcgaagctc gtcgtccgaa
    12241 agagccggca atcctggctg aaatcagcgg tatcgtttcc ttcggtaaag aaaccaaagg
    12301 taaacgtcgt ctggttatca ccccggtaga cggtagcgat ccgtacgaag agatgattcc
    12361 gaaatggcgt cagctcaacg tgttcgaagg tgaacgtgta gaacgtggtg acgtaatttc
    12421 cgacggtccg gaagcgccgc acgacattct gcgtctgcgt ggtgttcatg ctgtgactcg
    12481 ttacatcgtt aacgaagtac aggacgtata ccgtctgcag ggcgttaaga ttaacgataa
    12541 acacatcgaa gttatcgttc gtcagatgct gcgtaaagct accatcgtta acgcgggcag
    12601 ctccgacttc ctggaaggcg aacaggttga atactctcgc gtcaagatcg caaaccgcga
    12661 actggaagcg aacggcaaag tgggtgcaac ttactcccgc gatctgctgg gtatcaccaa
    12721 agcgtctctg gcaaccgagt ccttcatctc cgcggcatcg ttccaggaga ccactcgcgt
    12781 gctgaccgaa gcagccgttg cgggcaaacg cgacgaactg cgcggcctga aagagaacgt
    12841 tatcgtgggt cgtctgatcc cggcaggtac cggttacgcg taccaccagg atcgtatgcg
    12901 tcgccgtgct gcgggtgaag ctccggctgc accgcaggtg actgcagaag acgcatctgc
    12961 cagcctggca gaactgctga acgcaggtct gggcggttct gataacgagt aatcgttaat
    13021 ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta tggggggagt ttagggaaag
    13081 agcatttgtc agaatattta aggaatttct gaatactcat aatcaatgta gagatgacta
    13141 atatcctgaa actgactgaa ctaattgagt caaactcggc aaggattcga tactattcct
    13201 gtgtaacttt cttaaggaac gagaatgaaa caggaagtgg aaaagtggcg accttttgga
    13261 catccggatg gtgatattcg tgatttatca tttcttgatg ctcatcaggc tgtctacgtt
    13321 cagcatcatg agggcaaaga gcctttagag tatcgctttt gggttaccta ctctcttcac
    13381 tgcttcacaa aagattatga acatcagacg aacgaagaaa aacaatcgtt aatgtaccac
    13441 gcgcctaaag aatctcgtcc cttctgccag caccgttata acttagcgcg cacacactta
    13501 aaaagaacta ttttggcgct gccagaaagc aacgttattc atgccgggta tggtagctat
    13561 gccgtgattg aggtggactt agacggagga gataaggcat tttactttgt tgcgttcagg
    13621 gctttcaggg aaaagaaaaa actccgtttg catgtaacta gcgcttatcc catttctgaa
    13681 aaacagaaag gtaaatcagt gaaatttttc accattgcct acaacttatt gagaaataag
    13741 cagcttcctc agtcctcaaa ataacaaaac ccaccttaag gtgggtttcg ccagagaatt
    13801 atctctggta ttcagaacgc cattaccgga ctttgccttg accttgcgat aatcgcaggt
    13861 tgcgggatgt ctgaatttct tcagtctgct gcatcctgga agatgagaac atgtgttctt
    13921 attttcgtct ctatcatagt tgagtattta ctctcttaca atcagatctc tttcattgct
    13981 caacaggcga tggcttcaga ctttgcatta cggaattttt aagaaaggca gggcgaaacg
    14041 aggaagaagc ttttcgccct ggacaacatt cctgctgacg gcactaccat aaaaaaacag
    14101 aacgctttca acaaccttgc gtttttcatc ttccgtagcg gctcggaata aatttacgtt
    14161 cttacattcc gtctcatagt ctttgcgagg cccgtcccag atagctgtcc cagtctttcc
    14221 ataccggctg caaaccctga gcggttaacg cggcagcaac cgcttccggt ctgcgatcgt
    14281 cgtgcggtga gaactgttcc aactcggggt gattatcggc atagccacct ggctgcgttt
    14341 tcgagaaggc gctgacgtta ttgatcgcca gcggaataac gcgatcgcga aaccacggtg
    14401 attcccgcgt ggagagtgac agttcaatct ccggtgcaag cagtcggaag gcgcagatgg
    14461 tttgcactaa ctggcgttca tccataatcg acgcaggctc aatgccgcca gtacacgggc
    14521 gcaggcgcgg aaaggagaca gagtaacggc tttgccagta atgctgttgc agccatagca
    14581 aatgttcggc aaccatatag cagtcaacgc gccagttgtc ggaaaggcca attagcgcgc
    14641 cgaggcctat cttatcaatc cccgcacgcc ccagccgatc cggcgtttcc agccgccaga
    14701 agaagtcctg tttttttcct ttcagatgat ggcgggcata agtcgcctcg tgatatgtct
    14761 cctgataaac catcacgcca tccagaccaa gctgctttaa ctcggcgtat tccgtctccg
    14821 ccagcggttg cacttccatt tgtagtgaag agaactgttc acgcagggcg gggagatgac
    14881 gacgaaagta atccatcccc actttcgcct gatgttcacc agtgactaat agcagatgtt
    14941 caaagcccat ctcccgtata gcggcgcttt ccctggcaat atccgcttca tccagcgttt
    15001 tgcgcttgat gcgattactc atggaaaatc cgcagtacgt gcagtcgtta gcgcaaagat
    15061 tggaaagata aagcgggacg tagaaactaa ctgtgttgcc aaatcgctga cgggtcagac
    15121 gctgcgcccg ttgggccagt tgttccagat agccactggc ggctggcgat aacagcgcca
    15181 tcatatcgtc gcgggtgagt tgcgaggcat ttagcgcccg ttctacgtca gcagccgttt
    15241 tgccgttgat acgcaggcgg atgtcgtccc agtccagttg tcgccagcga tcgctgaagg
    15301 ttttcatgcc gatgcctcca gaaatccagt cagcgggctg gtggcatgag caaaatgact
    15361 gcggctgccc ggtccggact gacgtgccag caggcctgct tctaccgcca gacgaaatgc
    15421 cttcgccatg ttgacgggat cgtccgcgac ggcaattgcc gtattcacta acaccgcgtc
    15481 ggcccccatt tccagcgcct gcgcggcatg gctgggaacg ccgatgccag catcgacaac
    15541 caccggcact gtggcctgct ggataataat ctccagcatg gcgcgggttt ccagtccctg
    15601 attcgagcca atcggcgcgc cgagcggcat caccgctgca cagccgactt cttccagacg
    15661 tttacacaat accggatcgg ccccgcagta aggcagcacg acaaatccct gttgtaccag
    15721 cgtttcggcg gctttcaggg tttcgatggg atcgggcaac agccagcggg cgtcagggtg
    15781 aatctctaat tttaaccagt ttgtgcctaa cgcttcacga gccagatggg cggcgaaaat
    15841 ggcttcttcc gctgttttcg ccccggatgt atttggcagc agggtcacac ccgccgcgat
    15901 aagcggttcg aggatagcgt cgttgtgctg gcgcaagtcg acacgtttca tcgccagtgt
    15961 caccagctgg ctgccggaag cgcggatcgc ctccaccatc agttgtgaag aagcgaattt
    16021 ccctgtgccg gtaaacagat gtgaatcaaa cgttttgtcc gcaatacgta acatttcaac
    16081 cccctgcaat aacctgaaaa agcaggatct ggtcgccatc ctgcacgata tgttgcgccc
    16141 actgctcacg cgggacgatt tgctgattaa tcgccagagc cgcgcccgct tgtcgttggt
    16201 cgagttgctc cagtagttcg tgaacagttt gcccggcggc gcactgcatc ggttgatcgt
    16261 taaacaggat ctgcattgct tcctccgcat accgggcaac cactggcgcg gcgcaacgcc
    16321 aggctgcgcc actggctcga tttaccgtcg aacagtcgga gttctcccgc aggtgtctct
    16381 ataccgctta ataacttaat ggcttccagt gcctgcaaag tgcccataac cccgaccacc
    16441 gggccaacca cgcccgccgt gcggcagttg cgttctggct cctggttatc tggccacagg
    16501 cagcggtaac acccctgctc ccagggcggc gtcagtacca tcaactgacc gccaaatccg
    16561 accgcgctgg cggtgataag cggcgtgttg agtgccacgc aggcggcatt aatctcctgg
    16621 cgagtcgcca tattgtcggt acagtcgagc accacatcgg cccgtgcaac cgcatctttt
    16681 aacgcctcac ccgttaaccg ttgttgtaat gctgtcagtt gaatgtcggg attcaactgt
    16741 gtcagtcgct gttggctgac ctgcgatttc gggcgatcga tatcttcagt ggtaaagagg
    16801 atttgtcgtt gcagattgct taaatgcaca tcgtcgtcat ctgccagtac cagcgtcccg
    16861 acgccagcgc ccgccaggta cagcgcagca ggtgtaccca gcccgcccag accgataatc
    16921 agcacctggc tgtcgagcag tttttgctgc ccgtccagag cgatatcgtc gagcaggatt
    16981 tggcggctat aacgcataaa gtcacggtca ttcatcgcca actcctgcaa tttccagcaa
    17041 ctgtgccgtt gccaaacgcc agtctgcagc ttgagtaatg gcgctgacga cggcgatact
    17101 gccgacaccc gttgctatca ccgcaggcgc gcgtgccaga ctgataccgc caatcgccac
    17161 ggtgggataa tccgccagtc gctcaacatg ccgtgccagc tgttccagcc cctgcggtgc
    17221 agaaggcatc tgtttggttt gcgtcgggaa cacatgtccc agcgcgatat aagaggggcg
    17281 tgctgccagc gcgacgtcga tttccatatc gtcatgtgtc gaaacgccca gccgcaggcc
    17341 tgccgcgcgg atggcattga gatcggtggc ttgcaaatct tcctgcccca aatggacgcc
    17401 atacgcctga tgcttgatcg ccagccgcca gtaatcgttg ataaacaatc gcgcgttata
    17461 gcggcggccc agcgcaattg ccgccacgac atcggcttcc acctcttcat cgcgccgatc
    17521 tttgatgcgt agctggagag tacgtacgcc tgcatccaac agacgttcga tccactgtac
    17581 gctgtccacc accgggtaca gtcctgaacg aaaaggtaca ggaggaaaat caggctgata
    17641 catcacgctt cctccttacg caggtagatt tctccgcctc tggcacggaa gttctccgac
    17701 atatccgcca ttcccatttc aatagtttgc gtggcggcgt aatcacgcac ttcctggctg
    17761 attttcatcg agcagaattt cggcccacac atggagcaaa aatgggcgac tttacctgac
    17821 tcttgcggca gggtttcatc gtgataagcg cgggcggtaa acgggtcgag ggccagatta
    17881 aactggtctt cccagcgaaa ttcgaagcgg gctttcgaca tggcgttatc gcgaatttgc
    17941 gcgcccggat gccctttcgc caggtcagcg gcgtgggcgg caatcttata ggtgataagc
    18001 ccctgcttaa catcttcttt attgggcaga cccagatgct cttttggcgt tacgtaacag
    18061 agcatcgcgc agccaaacca gccaatcatc gccgcaccaa tccccgacgt gaagtggtca
    18121 tagcccggcg caatatcggt agttagcggc cccagagtgt aaaacggcgc ttcgtggcag
    18181 tgctctaact cctcggtcat attgcggcgg atcatctgca tcggcacgtg gcctgggcct
    18241 tcaatcatca cctgcacgtc atattcccag gcaattttgg tcagttcgcc cagcgtatgc
    18301 agctcggcaa actgcgcttc atcgttggcg tcctgaatag aaccggggcg cagaccgtcg
    18361 cccagcgaca gcgaaacgtc ataagcggca cagatttcac aaatttcgcg gaagtgttga
    18421 tagaggaaat tttcctgatg atgggagagg caccatttcg ccataatcga accgccgcga
    18481 gagacgatac cggtcaggcg tttcgcggtc atcggcacat agcgcagcag tacgcccgca
    18541 tggatagtga agtaatccac accttgctcg gcctgttcca gcagcgtgtc gcggaacgct
    18601 tcccaggtaa gatcttcggc gatcccgtta accttctcca gcgcctggta gatcggcact
    18661 gtaccgatcg gcaccgggct gttacgcaaa atccactcgc gggtttcgtg aatatagcga
    18721 ccggtggaga gatccatcac cgtatccgct ccccagcgcg tggaccatac cagcttttcc
    18781 acttcttctt cgatggaaga ggtgaccgcc gagttgccga tattggcgtt aacttttacc
    18841 aggaaattgc gaccaataat catcggctcc gattccggat gattaatgtt ggccgggata
    18901 atcgcacgtc cggcagcaac ttcatcacgg acaaattccg cagtgatatt ttccggcaga
    18961 cgtgcgccaa agctcattcc cggatgctgg tggcgtaaaa cttcgctacg gatgcgctcg
    19021 cggcccatat tctcgcggat ggcgatgaat tccatttccg gcgtgatgat gccctggcgg
    19081 gcgtagtgca gttgggtgac acggcgtcct gctttggcgc gttttggtgt tagtacgccg
    19141 ctaaaacgca gttcgtcgag gccatcatct gccagccgcg ctttagtgta atcggaactg
    19201 cgcacggtaa gttcttcggt atcgccgcgc gcatcgatcc acggctggcg tagttttgcc
    19261 agcccttgct gcacgttaat ggcaatctgc ggatcaccat acgggccgga ggtgtcgtag
    19321 accggaatcg cttcgttttc ttcgtactgc ggctgttctt tgctaccgcc aattagcgtc
    19381 gggctaacct ggatctcacg catcggcacg cgcacgccgg ggtgtgtgcc agtgatataa
    19441 atgcgttttg agttgggaaa ggcggtgcct tccagggtgt cgataaaatg ttgggcccgg
    19501 gcgcgttgtt cgcggcgggt cagttttgtt gcagacatag ctcattccaa aaagttaagg
    19561 acgtggcttg tcagacgacg gatgaagcaa gagacgatcg ccgcaggggc gatgcgatag
    19621 cagattaact cttgttccct tcgcaggtat tagcctgatc aggttccgcg gatcccgaat
    19681 aaacggtctc agccagttaa ggcactccga caagaaatta gccccgcaaa tggggcattg
    19741 aatgtaaatt acgcgttaac agcgcagaac tcaagcagga tgtttgacgc gggcggcgtc
    19801 aagcaccagc agaatcagct tatcttccag cacaaagcgc gcttccagcg cctcgccgat
    19861 gtcagataaa acctgttgaa actcaaggta attatcatga tcgatggcgg tttccagact
    19921 ggaatcgtag taatccataa tctgttgagt attggcttcg agttgcggcc aaatcttcgc
    19981 ggcgcgcgcg agttgcccgt tgccttccag cttatgaaga atgcgctcat aaatactgaa
    20041 atgtccggca gacaagtaat cgaccaggct ctgacaaaaa tcatcaaggg ctttttcgtt
    20101 tagcctcatg tacgattctt tgccaggctt aatgccaacc agattgtagt aagccacgag
    20161 cagatgctta cgtacatgta gccagcgatc aaccagtttg ttacttcctc tgacgcgctc
    20221 cgtcaggtta tcgagctggt taagcatgat tgactccgca agtttgtatt caaaaactgc
    20281 tcagtgagaa atgtaaaaac catgttaaac atgccagtga tgcaaaggta gtgcaagagc
    20341 tatggatcgt ataattgaaa aattagatca cggctggtgg gtcgtcagcc atgaacaaaa
    20401 attatggttg ccgaagggag aattgccata tggcgaagcg gcaaatttcg atcttgtggg
    20461 tcagcgcgca ctacaaatcg gcgaatggca gggggaacct gtttggttag tacaacagca
    20521 gcggcgtcac gatatggggt cggtacgtca ggtcattgat ctcgatgttg ggctgtttca
    20581 actggccgga cgaggcgtac aactggcgga gttttaccga tcgcataaat actgtggtta
    20641 ctgcgggcat gaaatgtatc cgagcaaaac cgaatgggcg atgctgtgca gccattgccg
    20701 tgagcgttac tacccgcaaa tcgccccctg cattattgtt gccatccgtc gcgatgattc
    20761 gatcctcctc gcccagcata cccgccatcg taacggtgtc catacagtac ttgccggatt
    20821 cgtcgaagtg ggcgaaaccc tcgagcaggc agtcgcgcgg gaagtgatgg aagagagcgg
    20881 aattaaagtt aaaaacttgc gttacgtgac ttctcagccg tggccgtttc ctcagtcttt
    20941 aatgaccgcg tttatggcgg aatatgacag cggcgacatc gtgatcgacc cgaaagaatt
    21001 gctcgaggcg aactggtatc gctatgacga tttgccgtta ctcccgccgc ccggcaccgt
    21061 agcgcgccgt ctgatagaag atacggtggc gatgtgtcgg gcagagtatg agtgatgata
    21121 cactgaccgc ctgacgcact aaggaacagc caaaatgacc gaacttaaaa acgatcgtta
    21181 tctgcgggcg ctgctgcgcc agcccgttga tgtcactcca gtatggatga tgcgccaggc
    21241 gggtcgctat ctaccggaat ataaagccac gcgcgcccag gcgggcgatt ttatgtcgct
    21301 gtgcaaaaac gccgagctgg cgtgcgaagt gactttgcaa ccgctgcgtc gctacccgct
    21361 ggatgcggcg atcctctttt ccgatatcct caccgtgccg gacgcgatgg ggttagggct
    21421 ctattttgaa gccggagaag gtccgcgttt tacctcgcca gtcacctgca aagccgacgt
    21481 cgataaactg ccaattccgg acccggaaga tgagctgggt tacgtgatga acgcggtgcg
    21541 taccattcgt cgcgaactga aaggcgaagt gccgctgatt ggtttttccg gcagcccgtg
    21601 gacgctggcg acctacatgg tggaaggcgg cagcagcaaa gccttcaccg tgatcaaaaa
    21661 aatgatgtat gccgatccgc aggcgctgca cgctctgctc gataaactgg cgaaaagcgt
    21721 cactttgtat ctgaatgcgc agattaaagc cggtgctcag gcagtgatga ttttcgacac
    21781 ctggggcggc gtgcttaccg ggcgcgatta tcaacagttc tcgctctatt acatgcataa
    21841 aattgttgat ggtttagtgc gtgaaaacga cggtcgccgc gtaccggtca cgctgtttac
    21901 caaaggcggc ggacagtggc tggaagccat ggcagaaacc ggttgcgatg cgctgggcct
    21961 cgactggaca acggacatcg ccgatgcgcg ccgccgtgtg ggcaataaag tcgcgttgca
    22021 gggtaatatg gatccgtcga tgctgtacgc gccgcctgcc cgcattgaag aagaagtagc
    22081 gactatactt gcaggtttcg gtcacggcga aggtcatgtc tttaaccttg gtcacggcat
    22141 tcatcaggat gtgccgccag aacatgctgg cgtgttcgtg gaggcagtgc atcgactgtc
    22201 tgaacagtat caccgctaag gagtgattat ggatctcgcg tcattacgcg ctcaacaaat
    22261 cgaactggct tcttctgtga tccgcgagga tcgactcgat aaagatccac cggatctgat
    22321 cgccggagcc gatgtcgggt ttgagcaggg cggagaagtg acgcgagcgg cgatggtgct
    22381 gctgaaatat ccctcgcttg agctggtgga gtataaagtt gcccgcatcg ccaccaccat
    22441 gccttacatt ccaggttttc tttccttccg cgaatatcct gcgctgctgg cagcgtggga
    22501 gatgctgtcg caaaagccgg atttagtgtt tgtcgatggt catgggatct cgcatcctcg
    22561 ccgtcttggc gtcgccagcc attttggctt attggtggat gtgccgacca ttggcgtggc
    22621 gaaaaaacgt ctctgcggta aattcgaacc gctctccagc gaaccgggcg cgctggcccc
    22681 gctgatggat aaaggcgagc agctggcctg ggtctggcgc agcaaagcgc gctgtaaccc
    22741 gttgtttatc gctaccggcc atcgggtcag cgtggacagc gcgctggcgt gggtacaacg
    22801 ctgcatgaaa ggctatcgtc tgccggagcc aacgcgctgg gcggacgcgg tggcctcgga
    22861 acgtccggcg ttcgtgcgct atacagcaaa tcagccctaa ttcaggtaaa ctgcggataa
    22921 tttccgtatt tgagaactca acatgttaca aaacccaatt catctgcgtc tggagcgcct
    22981 ggaaagctgg cagcatgtca ctttcatggc ttgcttatgc gaacgcatgt accccaatta
    23041 cgccatgttc tgccagcaaa ccggttttgg tgatgggcaa atttaccgtc gtattctcga
    23101 tctcatctgg gaaacgctga ccgttaaaga cgcaaaagta aatttcgaca gccaactgga
    23161 gaaatttgaa gaagcgattc cttcagccga cgatttcgat ctgtacggcg tttatccggc
    23221 aatcgatgcc tgcgtggcgt taagtgaact ggtccattcg cgtttgagtg gtgaaacgct
    23281 cgaacacgcg gtggaagtga gtaagacctc catcacgacc gttgcgatgc tggaaatgac
    23341 tcaggctggt cgcgaaatga gcgatgaaga gcttaaagaa aacccagctg tagagcaaga
    23401 atgggacatt cagtgggaaa tattccgact tttagctgag tgcgaagaac gcgatatcga
    23461 gctgataaaa ggccttaggg cagacctgcg tgaggcgggt gagagcaata ttggtataat
    23521 ttttcagcaa taagaccaga aaacgtgatt taacgcctga tttgtcgtac ctggagtctt
    23581 ccctttcgcc ccccgtctgg tctacatttg gggggcgaaa aaaagtggct atcggtgcgt
    23641 gtatgcagga gagtgctatt ctggcatttc cgtcgcactc gatgcttagc aagcgataaa
    23701 cacattgtaa ggataactta tgaacaagac tcaactgatt gatgtaattg cagagaaagc
    23761 agaactgtcc aaaacccagg ctaaagctgc tctggagtcc actctggctg caattactga
    23821 gtctctgaaa gaaggcgatg ctgtacaact ggttggtttc ggtaccttca aagtgaacca
    23881 ccgcgctgag cgtactggcc gcaacccgca gaccggtaaa gaaatcaaaa ttgccgcagc
    23941 taacgtaccg gcatttgttt ctggcaaggc actgaaagac gcagttaagt aagattgcgt
    24001 ggcagtgaac agttttaacg aaggggtggt ttcacccctt ttgtctttct ggcgtcgatc
    24061 attgatgctg gcaggtgcgc tgtttctcac tgcctgtagc cacaactctt cacttcctcc
    24121 ctttaccgcc agtggatttg ctgaagacca gggcgcggta cgcatctggc gaaaagacag
    24181 cggcgataat gtgcatctgc ttgccgtgtt tagcccgtgg cgcagtggcg ataccacgac
    24241 gcgagagtat cgctggcagg gcgataacct cacgctcatc aatatcaatg tttacagcaa
    24301 accgccggtg aacattcgcg cgcgttttga cgatcgcggt gatctgagct ttatgcaacg
    24361 tgaatccgat ggggaaaagc agcagctttc taacgaccaa atcgatttat accgttatcg
    24421 tgctgctcag atccgccaga ttagcgatgc cttacgtcag gggagagtcg tgctgcgcca
    24481 ggggcgctgg catgcgatgg aacagaccgt gaccacctgc gaagggcaaa ccattaaacc
    24541 tgatttagat tcgcaggcga tagcgcatat cgagcgccgc cagagccgct cttctgttga
    24601 tgtcagcgtg gcatggctgg aagcgcccga aggttcgcaa ttactgttag tggcaaactc
    24661 tgatttctgt cgctggcaac ccaacgagaa aacgttctga tttaccagtg gcccataccc
    24721 atatgaccgc caccaccgca gccgccgtag cccatgccca ttccggctcc gcgcggaata
    24781 cccgcttcag ccatcgcgat atcccgtttc acccgtaact catctaacga ctgacgcaaa
    24841 ttctccatct ctttggcgac cgcgttaatt ttgctgctat ccggtgggtt cgcggctaac
    24901 agggcattgt attcataacg cttcgtcacc agttgctgtt gcagtgcgct gctttgagtg
    24961 taaaagtcat tatggatttt ctgccacgct gtctgttgtt cgctggtcaa aggcgcggca
    25021 ttttgctgcc acataccgtg tccgccgtga gcaaatgcag atgtcgatcc catcgccatt
    25081 gctgaaagcg ccatcattac cagggcaatt ttcgtgttcc gtttcatggt taatcctcca
    25141 gtggttgtct tacttcgggt attgcatctt ccgtgccaac gatgaaacgc tgatatgacg
    25201 ggtaatctgg catgataaac gagtaaaaat gactcgcctg ctgcgggtag cgagtcattt
    25261 ttactcattg aaacttaagc ctttgtgtta cagcgcaggg taagcgctga taaaagatgg
    25321 catgatttct gctgtcagaa agggatgagc aggcaaagaa gaagatgcgt tttatgcaac
    25381 gttctaaaga ctccttagct aaatggttaa gcgcgatcct ccccgtggtc attgttgggc
    25441 tggtagggtt gtttgcggtg acggtgattc gcgattatgg gcgcgagact gccgccgcca
    25501 gacaaacgct gctggaaaaa ggcagtgtac ttattcgcgc tcttgaatcc ggctcgcgcg
    25561 tcggcatggg gatgcgcatg catcatgcgc agcagcaggc cttactggaa gaaatggccg
    25621 ggcagcctgg agtacgttgg tttgcggtca cggatgaaca aggaacaatc gtgatgcata
    25681 gcaactccgg catggtggga aaacagcttt attccccgca ggaaatgcag cagttacatc
    25741 cgggagatga agaagcgtgg cggcggatcg atagcgcaga cggcgagcct gttctggaaa
    25801 tttatcgcca gtttcaaccg atgtttgctg ctggaatgca ccggatgcgc catatgcagc
    25861 agtatgccgc gacaccacaa gcaattttca tcgctttcga cgccagtaat attgtgagtg
    25921 ccgaagatcg tgagcagaga aacaccctga ttatcctctt cgccctggcg acggtcttgc
    25981 tggcaagcgt attgtcattc ttctggtatc gccgctatct gcgctcgcgc cagcttctac
    26041 aagatgaaat gaagcgcaaa gagaagctag tggcgctggg gcatcttgcg gcaggcgttg
    26101 cccacgaaat ccgtaaccca ctttcctcga ttaaaggact ggcgaaatac tttgccgagc
    26161 gcgcgcctgc agggggagaa gcgcatcaac tggcgcaggt gatggcgaaa gaggccgacc
    26221 gtttaaaccg cgtggtaagc gagctgctgg aactggttaa gccaacgcat ctggctttgc
    26281 aggcggtgga tctcaacacg ctgattaacc actcattaca gctggtaggc caggatgcaa
    26341 acagccggga gatccagtta cgctttaccg ccaacgacac attaccggaa attcaggccg
    26401 atccggacag gctgactcag gtcctgttga atctctatct caatgctatt caggcgattg
    26461 gtcagcatgg cgtgattagc gtgacggtca gcgaaagcgg cgcgggcgtg aaaatcagcg
    26521 ttaccgacag cggtaaggga attgcggcag atcagcttga agccatcttc actccgtact
    26581 tcaccaccaa agccgaaggc accggattgg ggctggcggt cgtgcataat attgttgaac
    26641 aacacggtgg tacaattcag gtcgcaagcc aggagggaaa aggctcaacg ttcaccctct
    26701 ggcttccggt caatattacg cgtaaggacc cacaaggatg acgcacgata atatcgatat
    26761 tctggtggtg gatgatgaca ttagccactg cactattttg caggctttac tgcgcggctg
    26821 gggctataac gtcgcgctgg cgaacagcgg gcgacaggcg ctggagcagg tgcgggagcg
    26881 ggtttttgat cttgtgcttt gcgatgtgcg aatggcagag atggacggca ttgccacgct
    26941 aaaagagatc aaagcgttaa atccggcgat tccggtactg attatgaccg catactccag
    27001 cgtcgagacg gcggtagagg cactgaaaac cggggcgctg gattatctca tcaagccgct
    27061 ggatttcgac accctgcagg cgacgctgga aaaggcgctc gcgcatacgc acagtattga
    27121 tgctgaaacg cctgcggtga ctgccagcca gttcggtatg gtcggtaaaa gcccggcgat
    27181 gcaacacctg ctcagtgaaa tcgccctcgt cgcgccatcg gaagccacgg tgctgatcca
    27241 cggcgattcc ggcaccggta aagagctggt cgccagggcg attcacgcca gtagcgcacg
    27301 tagcgaaaaa ccgctggtaa cgctcaactg tgcggcactc aacgaatcct tgctggaatc
    27361 tgaattgttc ggtcacgaaa aaggggcgtt tactggagcc gataaacgcc gggaagggcg
    27421 ctttgttgag gcggacggcg gcacgctgtt tctcgatgaa attggcgata tctcgccgat
    27481 gatgcaggtg cgtctgctgc gtgcgattca ggagcgcgaa gttcagcgtg tcggtagcaa
    27541 ccagactatc tcggttgatg tccggctgat tgcggcgacc catcgcgatc ttgccgcaga
    27601 ggtgaatgcc gggcgttttc gccaggatct ctactatcgc ctgaatgtgg tggcgattga
    27661 agtaccatcg ctgcggcaac ggcgggagga tatccctctg ctggctagcc attttcttca
    27721 gcgttttgcc gagcgtaatc gcaaggcggt aaaaggcttt acgccacagg cgatggattt
    27781 gctgatccac tacgactggc cgggaaacat tcgtgaactg gaaaacgcgg tggaaaggtc
    27841 ggtggtgctg ctgaccgggg aatatatttc cgaacgcgag ctgccgctgg cgattgccag
    27901 tacgccgatc ccgctggggc aaagtcagga tattcagctg ctggtggaag tcgaaaaaga
    27961 agtgattctg gcggcactgg agaaaacggg cggcaacaaa accgaagccg cccgtcagtt
    28021 agggatcacg cgcaaaacgc tattggcaaa actgtcgcgt tagttctgct cgcgttcgat
    28081 agcgcgccag ccgatatctt tccggcagaa gcagtcgtcc cagtgaatat tggtcattaa
    28141 ggcataagcg cgtttctgcg cttctgccac ggtatgaccc agcgcggtga cgcacagtac
    28201 gcgcccgccg ctggtcacta cctgctcgtt atccgccagt ttcgtgcccg cgtggaacac
    28261 tttgccgcct gccacttctt ccagcggcag gccgtggatc acgtcaccgg tgcggtaatc
    28321 acccggatat ccacccgcag ccatcaccac gccgagagaa gcgcgttcat cccactctga
    28381 cgttttctcg tccagtttgc cttcacaggc cgccaggcag agctcaacca gatcggactt
    28441 catgcgcagc ataatcggct gggtttctgg atcgccaaag cggcagttaa attcgataac
    28501 cttcggattg ccctgtttgt cgatcatcag gcccgcgtag agaaaaccgg tgtaggtgtt
    28561 gccttccgcc gccatgcctt tcacggttgg ccagatgata cgttccatgg tgcgctgatg
    28621 aacttcatcg gttactaccg gcgcggggga gtaagcgccc atcccgcccg tgtttggtcc
    28681 ggtatcttta tcgcctacgc gtttgtgatc ctggctggtg gccatcggca gcacatgctc
    28741 gccgtctacc atcacgataa agctcgcttc ttcgccatcg aggaactctt cgataacgat
    28801 gcgatgaccc gcgtcgccaa aagcgttgcc cgccagcata tcgtgaacag ccgcttccgc
    28861 ttcttccagc gtcatcgcca cgataacgcc tttcccggca gccagaccgt ccgctttaat
    28921 gacgattggc gcgcctttct cacgcagata cgccagcgca ggttctacct cggtgaagtt
    28981 ctggtattcc gccgtaggga tcttatggcg ggccaggaaa tctttggtaa acgcttttga
    29041 gccttccagt tgggccgcac ctgcggttgg gccgaagatt ttcagcccgg cggcgcggaa
    29101 ggtatcgacc acgcctttca ccagcggcgc ttccgggccg acgatggtca gatcaatctt
    29161 ttcgttttgt gcgaaatcca acagcgccgg gatatcggtc acgccaatag caacgttttg
    29221 cagcgcgggt tccagtgcag tgcctgcatt acccggagca acaaaaacag tctcaaccag
    29281 cggcgactgg gccgctttcc aggccagcgc gtgctcgcgc ccgccgttac caatcactaa
    29341 tactttcatc tattgctcca ttaatggcgg aagtggcgca tgtcggtgaa gagcatcgca
    29401 ataccgtgct cgtcggcggc ggcaatcact tcgtcatcac ggatggaacc gccaggctgg
    29461 attacgcagg tcacgcccgc agcggcggcg gcatcaatac cgtcgcggaa cgggaagaac
    29521 gcgtcagaag ccatcgagga acctttcact tccaggcctt catcggcagc tttaataccg
    29581 gcgattttcg cggagtacac gcggctcatc tggcccgcgc caataccgat ggtcatattg
    29641 tttttggcat agacgatagc attggatttc acgaacttcg ccactttcca gcagaacagc
    29701 gcatcgcgca gttcctgttc ggtcggctga cgttgggtga cgacgcgcag ttcttccgca
    29761 ccgaccatcc ccaggtcacg atcctgaacc agcagaccgc cgttcacgcg tttgaaatca
    29821 agacccggaa cacgctcgcc ccactgaccg caggtcagaa cgcgtacgtt ctgcttggcg
    29881 gcggtgattt tcagggcttc ttcgctggcg gaaggcgcaa taatcacttc gacaaactga
    29941 cgagaaatga tggcctgtgc ggtttccgca tctagctcgc ggttaaaggc aatgatgccg
    30001 ccgaatgcgg aggttgggtc ggttttgtac gcgcgatcgt aagcatcaag aatggaattg
    30061 ccgatagcta caccgcaagg gttggcgtgc ttcacaatca cacatgccgg ctcggcgaac
    30121 tctttcacgc actccagcgc cgcatcggta tcggcgatgt tgttataaga gagggcttta
    30181 ccctgaacct gggttgcggt agcaacggag gcttctttca cattctcttc tatatagaag
    30241 gcagcctgct ggtggctgtt ctcgccgtaa cgcatatcct gcttcttaat gaagttcagg
    30301 ttcagcgtgc gtgggaagcg accagcggct tctttgcttt caccgtggta cgccggaacc
    30361 atgctgccga agtagttggc aatcatgctg tcgtaggcgg cagtgtgttc gaaggctttg
    30421 atggcgaggt cgaaacgggt tgcaagcgtc agcgatcctt cgttgtcatc catctcttta
    30481 ataatggcgt cgtagtcgct gctcttcacc acgattgcga catctttatg gttcttagcg
    30541 gcggagcgca ccatggtcgg gccgccgata tcgatgttct caaccgcatc ttccagcgag
    30601 caaccttcac gggccacggt ctgggcgaac ggatacaggt taacaaccac catatcgata
    30661 ggctggatct gatgttcttc cataatggca tcgtcctggc cgcgacggcc cagaatgcca
    30721 ccatgtactt tcggatgcag ggtcttcacg cgtccatcca tcatctccgg gaaaccggtg
    30781 taatcggaaa cttcggttac cggcagacct ttttctgcta acagacgggc agtgccccct
    30841 gtagacagca gctccacacc gcgtgcggaa agtgcctggg cgaattcgac gataccggct
    30901 ttgtcagaaa cactgagcag agcgcggcgg actggacgac gttgttgcat ggtaaatccc
    30961 ctggatttga ctattacaga gagcgttagc tgaatttttc gcgaaaaact cagctaacgc
    31021 ccctaacggg gcatccttat ttttcgcccg cattgtaacg aaaacgtttg cgcaacgctc
    31081 gcgaattttt ctctttcaat ggtgatcaca attttgacag tggttaccgt gggaaaaata
    31141 cagaaattac attgatgatt gtggataact ctgtgcgtaa aaaggtataa agcggggttt
    31201 tgctggggaa tgcagcagtc agtcattttt ctgcaatttt tctattgcgg cctgcggaga
    31261 actccctata atgcgcctcc atcgacacgg cggatgtgaa tcacttcaca caaacagccg
    31321 gttcggttga aga
//
LOCUS       DAZGVQ010000046        30656 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000046 DAZGVQ010000000
VERSION     DAZGVQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.046"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            84..800
                     /gene="nanC"
                     /locus_tag="ACHLAG_002012"
     CDS             84..800
                     /gene="nanC"
                     /locus_tag="ACHLAG_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFP0655252.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            820..1926
                     /gene="nanM"
                     /locus_tag="ACHLAG_002013"
     CDS             820..1926
                     /gene="nanM"
                     /locus_tag="ACHLAG_002013"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFP0655253.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSATIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAALVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            1991..2971
                     /gene="nanS"
                     /locus_tag="ACHLAG_002014"
     CDS             1991..2971
                     /gene="nanS"
                     /locus_tag="ACHLAG_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP0655254.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPSIPDNAGVLIVPCCRGGSAFIAGSEGTYSERHGASHDACRWGSDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGVISDRFVEAILQFWREK"
     gene            complement(2979..3083)
                     /locus_tag="ACHLAG_002015"
                     /pseudo
     CDS             complement(2979..3083)
                     /locus_tag="ACHLAG_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3183..4055
                     /locus_tag="ACHLAG_002016"
     CDS             3183..4055
                     /locus_tag="ACHLAG_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFP0655255.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSHYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQQGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EVFVEWHRKEVFRG"
     gene            4225..6300
                     /locus_tag="ACHLAG_002017"
     CDS             4225..6300
                     /locus_tag="ACHLAG_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0655256.1"
                     /translation="MTLVDSVEAGRLTISELIDALAKDKNYTASRWYQRYRAFTTLLQ
                     QTSTFAEPATDGLVKQLWYERDNGIASIRQGVPSLAEYQQSLPLLRELTERIRQQPDE
                     ETYQYVGNALQQAKENGLLKRMYWSLRNRVFAAFSPENYTSTVDENAFNKAAEFLNQH
                     FHLGLVLTGNWLQKNYELKQAIHAQSPDTDPYYVNMAIWHLYELLRERDNEQKQEKVA
                     STTSITRSEPIENKIILHSPTNVIFFGPPGTGKTFRLQQKMKEYTSHAVPADRDAWLD
                     SRLESLNWMQVITLVLLDLGKRAKVRQIIEHMWFQRKALLNGRNGNLSNTAWAALQSY
                     TVPESLTVDYKNRREPAVFNKTDNSEWFLVDSQLEQVEDLVELYAELKRGPKSAEAIQ
                     RFAVVTFHQSYGYEEFIEGIRARSDESGNISYPIEPGIFMRLCQRANADPGHRYAIFI
                     DEINRGNISKIFGELISLIEVDKRAGMPNAMSLQLAYSGDHFSVPGNVDIIGAMNTAD
                     RSLALMDTALRRRFDFVEMMPDLSLLSEAKVKGIELESLLEKLNSRIEALYDREHTLG
                     HAFFMPVKNALDAGDEEAAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDD
                     NLQFVIEKTDDLDTLFGNNHGLRRHDQQSTAYELKDFDQEIWNIPQAYRSIYQPQQTP
                     LDEQAVNHG"
     gene            6293..7636
                     /locus_tag="ACHLAG_002018"
     CDS             6293..7636
                     /locus_tag="ACHLAG_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HFP0655257.1"
                     /translation="MGEVISVFEYDLLGSGKAASVGAKPVPPQVFNYLEALSLASNQG
                     SQFLKLTSRSGFKLLQVQNYAGMLSTPHGFQLEILPKVGKNLTAANARQTLLTMLSHL
                     PGFRHIQTQQATLQAQRMPLLEIFINQFLHSVSQLLKQGLRSDYVSKQGNLAFMKGKL
                     MLSAQLRHNAVNRHKFCVDYDDYMPDCAANRLLHSALDKLLSLQLSSDNQRWLYELRF
                     AFDGIPLSRDIERDINNLRLERGMAHYNEPMAWAQLILRGMSPSALQGNTKAISLLFP
                     MEAVFESFVAQTLPDELPPHLKVLPQAAIYSLVKHGVKDCFKLRPDLLIQSNKPVQTE
                     MVMDTKWKLVNSSQQTKSLYGLAQADFYQMFAYGQKYLGGNGEMYLIYPAHDDFSQPI
                     PQHFAFSETLKLWVVPFRIMAKRGERMMWPNDVSCTMSPQLDRMAASVSYSGGMR"
     gene            7633..8019
                     /locus_tag="ACHLAG_002019"
     CDS             7633..8019
                     /locus_tag="ACHLAG_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655258.1"
                     /translation="MTHEYQRQSLEQLITQQTATPRSAAVVFNENQTLWQTLGFSQSQ
                     VSLWLASLPQSNHLKGPTTATYQVIPDIAAHLVSLLHQAGGRMPLAQVLKKLPAGITT
                     SEQQIRKLAQKHAQIEIKGPLLVLIN"
     gene            8070..10217
                     /locus_tag="ACHLAG_002020"
     CDS             8070..10217
                     /locus_tag="ACHLAG_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP0655259.1"
                     /translation="MAKEAKKQDPQQQLEALFNKLVKQHSEFTQPLVELPEHEFAKAG
                     LIQGLVEGNLTPPVMLIALTEGHWEYAADTAKFDDLAYQLLSDIEGDWPVYAVVDDGL
                     NHRILALFGADGSDGTHGADTLPGLEELRSFDRMERDPTFRWSMRVYTRLMQRFDAFH
                     ENVYRVTKDKVNDKNDIIEEVAKLLFLETFRLHHDEDLTFKDDEGNTLRFRDVFNWQY
                     VESHGDKAVAQIKAAFEEFKSHENYVVISDDGSRNPIFSKETHLRLSVAKNYQDLLEA
                     IQNLGPVKTNDGKIAKEHGTLADVSGDLLGRVFDVFLRANFESKGGLGVYLTPNPVKQ
                     AMLEIAMHDIDDDDEMRSRLANGDFRFCDPTCGSFGFGSVALSQIDKWIDFKLVLADD
                     KKESLKQKLRDCAFTGADAAPRMVMLARVNMALQGAPKAQIFYTDNSLTTNALKPNSF
                     DLICTNPPFGTPKFDKGKNGQNSKANYEANMEQVLGGFRPTKKVVDSYNAWYDHVKMK
                     WEDIGDLELDENGEPKWAGYRTDLRRTGGTDKKPFYSLQPTTAGLALGSKPDSKGNWQ
                     PVGATIDPAVLFIDRCLQLLKPGGRLLIVLPDGILCNSGDRYVREYIMGKKDEKTGEF
                     VGGKAIVKAVISLPSDCFKLSGTGAKTSILYLQKRHANPNQPEQFLPEPQTDVFMAVA
                     ETLGYVVKNNIEDYNAGVANDLDKIVSAYKRGE"
     gene            10217..11743
                     /locus_tag="ACHLAG_002021"
     CDS             10217..11743
                     /locus_tag="ACHLAG_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP0655260.1"
                     /translation="MDKFFRTNSSILVERLDPNFYQPSLVENDKLIKSKGWVELQSLY
                     EASGIGNTSAVERYYSEAADSIPYISGKVIKSFNIDLDECQRISLDSHKNELTKSALK
                     PTDVLVIRKGDMGNACVVPSEVNEANCSSEVIYLKMKASSDPYYLVSYLNCDQGQKAF
                     KRLGRGTIIPGVSLLDVPRLPIPKVSEFVQKYIGDKVRQAEQLRAWAKLLRTSVDAHL
                     NSLNLPINEPPALLNRVSAQTMEDRLDPRPYRTHYLCLVREIEKLPHDSISTLVELAS
                     GCPVSSNDFLENSGIPLVRIRNIGFDDFIGLDTGVSQDVYQDATKYQAKDKMIVVGMD
                     GIFRSQFFISDELPMLVNQRVAMLSPQNIRGELLTHWLNRPEGQMQLNQWAVKTTVEH
                     TSLSDIGRVLIPRLDKSLENKLADYLLNARLAYRYAKFLTQVAKTLVEALIEGQLTEQ
                     QLIQAQQALEDGDNSFDQAILSKLSAEGYAIEGATPLFSDVDELYSLLEEAAQAEAEE
                     "
     gene            11761..13047
                     /locus_tag="ACHLAG_002022"
     CDS             11761..13047
                     /locus_tag="ACHLAG_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655261.1"
                     /translation="MDSITTRQNNDPVNSEAALNLCLQLWHQGGLTASKAALLLAAVP
                     ALRSLLQPIIQPKKKDAETDIISAFSLTAPLLDAFSDLSQSGEWQLALLGLNPDVRQH
                     WINLAAARCQEAGAMSDPMVLVKLIQQLGNASEWVLAQLENADFSPQIIASPLAQTER
                     DLLGHSLNDNAAIPALCRILRTSHTLFTVSEQNEPPASIQAVDVTAKQLTNNWCSGRL
                     LALPNTLLDEHNLKPNADWLLVSRSGHDNVPLTELFAQQPWLFLLSLIIFVQDAWAAE
                     QRGGLLLTLPAGQNAFAPGQINVAVQGIEGDEVSLGSLAEFLVLLLGELNIPLYPALD
                     ANTESINRLNRVLSSFIAELLAKKIWQFTEAGRGESGQYRIHTSFSDACYSLPLAPLF
                     GYKSQTLQRAIKQLAQNCYANKKRAANRINLQGSSL"
     gene            13044..17330
                     /locus_tag="ACHLAG_002023"
     CDS             13044..17330
                     /locus_tag="ACHLAG_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655262.1"
                     /translation="MSFELNEQMVDSLAKQHRWAATGVSDYPPRDPLVGQSRFFKRFQ
                     TFLHTVDHDDDRFAHVFAVEAEWGRGKSRLGHELVAQINDCSRGWFVRDEQGQLHDKQ
                     LFNRDTQDKYLALYIRYSQVASDYQNSDNWFAFGLYQALLPLATQTFDGSIQSEIAKQ
                     AYERLMPFGFKSELLADALQLNANHSEEDLYTDQTLVVSLVQAAYKVLKSFGVQYMLV
                     VLDELETVAEAATFGLEDEQNKRLDGQAIRLIGKAIKEEDPRRKLPWLRYVALCSPLL
                     GQQLREIQSVARRFELVELEHNAFADVSDYVASLLKDGKLGFNYPAGLVEAAYAMSGA
                     NFGWFNVVMANVDAVLSQYQQAGRSIKDVGDLFEAVLAGSGRVARHVLDKGAIEGIQT
                     RDQALLTECRSLLYGQLPVRLAEMAYATELLALKNEDLEPIAAHYRKLSIDRLQCRQA
                     LEQAKFRRDKDEWYYPAVDQALNLDTLLDNLRTFSIKEQAASGDKGAVLLPLSKGEFK
                     YLLSLLYDHPAIEFAADALWHKLVGETTELDASEATHIGPSVAMLLRLDLRYRSAQQN
                     SLIFKDPAMGDAHEQAMSELSKQSSQKPLIKALARVTGFVRLLDNNWTYDENLLSNKA
                     GSDDAPLAILTEPRGRQGGLLTLDGFKLHPEGKALFAWVSNLIELNNLHTFAANHSHQ
                     NGRTPVLALTASAHLMEQYSRLDERNELRDAILLHYVNPSEADQLERIGLSLAACQLH
                     GVNLTADSFTAKFKNKLHALTTFATDAIHKWRQRLQQRGLIAWPLKVDGKLSPNDRDL
                     LFKGWYQLAIAHPELNGILDLQQQHGVPVNELSSLLDKLKVPGSYIAKGYTADEHAGL
                     FTELNNVQRSQAQIPLFLARIAHPNKKHKWQFEGFKQQFYFAYVAETSVTAKGVFNDW
                     MWWCGELNLLTLTNPTEKQAIWEHYPRSRLENAIREAQNWFRGNDMGSYAANVEVMSR
                     VYGYARINEMFAPLGKNKLGFVTVEAKEQLEKAQSLFNVLKQQEEQLADMVEANDTKV
                     LAGLIHKRAEVLELVAKVKPLNSSRPMLKDAHILSLEDKTTSLYQRIEQACLFAEFVE
                     CSAERINNRLVDLIIDVETECAPLTNFPKRLYTNTLRTIGHILDGALKDDTSSATGRK
                     EQQADSDTLLHYLRKLDLGRAHDKLSALAQEVGLNLHNDQQLPIAEIQGHILSSYRNC
                     KERFSKLVNNLTEQKLRAQQLQEWLSSATAEYQFADDIAELPKLVMKLQLIEDAIADL
                     PSDVESKRQSMQNSLRNGQFSSLRDLPEELLKPARGQLTPIQGQLLKIEERLNQVRRN
                     SIAQVNSWLPLLKPLLASQKQAEPAALTLEDVSNLGVRELQQVCESTQAKWQSQGEQI
                     LNDTGLTLADWQPIYKALSQNQEPVLTPEQQKGLVDKGIVKMRLTFATGL"
     gene            17341..18189
                     /locus_tag="ACHLAG_002024"
     CDS             17341..18189
                     /locus_tag="ACHLAG_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655263.1"
                     /translation="MSRYLSAALASNRKGRFLQTVAGATPLMKDWISSLPASGLLIVQ
                     AEELTDANTMQHLYHWAMQAGCAALVINLKAEQFTLLAQLPYPLDWQLVPASLRGQEP
                     GLTALLASETDQAIAGFTGSADRYQHQAGDVVHTRYIRKHSNSGLLAFTTLPLWSLTL
                     LDHSELLVSWLNWFVDHAGIAERIIEPKAPSTDYTPDKHDLVVLLLLYAGGGMNLQAL
                     SEHNAVKLMFDVNSLDIVKRGEMLRQHDFIDDAGITATGKTCLQASQYWAYAPLLGEQ
                     LHTGTL"
     gene            18192..19349
                     /locus_tag="ACHLAG_002025"
     CDS             18192..19349
                     /locus_tag="ACHLAG_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655264.1"
                     /translation="MNENKTMNLAQSLAIDSQLRLPGSAGRLLKQVRDIEQVAPLFRD
                     AGLVKAVPKPTIVNLRVAGIATKSCLDKVLGGFIYASAAVRTDLLIDDNKVSTVGSDS
                     VSELDDIDFEAQRKRLDLIEIRQAYQLVEKKLLGYEPGDLILLDTPLFLARDMAPLER
                     NVRHKQEYEKTRQVIETFWQNHRSRIFPWNQNGVVLASILAERFSAIVSIAKQDLRTE
                     QGRKQILASDGFSKELMQNLEGLDESLVGVGDLRFINGILGNYSRTIAFRLSEQEKRI
                     EPNIEAKQGLIGFHFRSARGGQIKMVQLAGDEPEWKSADLDLVASRLMVLDMQNKGNA
                     MPLPQLLGYQQLGILPKFATFYRQGLHQALKNNDIEAGWLAGLDEGLNNGL"
     gene            19339..21024
                     /locus_tag="ACHLAG_002026"
     CDS             19339..21024
                     /locus_tag="ACHLAG_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HFP0655265.1"
                     /translation="MAYKVLGKLVGNTGDASQLTMVVQDSFSVRRGEFVRIMHQERKD
                     EGQVAVLGRVTKISRTNMLYNAGFGDGVTELELLPGANVTGENMFAQVELVGYRDPVT
                     RQIKIPRRPLNPGTAVETVDFQFLSDFYEFNEHSSLHLGNLVGYDKGENTVPVFIDVN
                     KLVTEHLAVLAMTGSGKSYTVGRIIERLVAVNNGTVVVFDPHGEYGKALAGGNLQFSS
                     FLEATDDKRDQEALPLIKQTFEKLQAAGAGIQVYSPQHESFKHKYASKNTPLALQFDH
                     FEMDDIAEILPGLTEPQQRVLDVAIRYWRYADKTEPRDINRLRFLLGDGIEELKEWDD
                     LSEAEAKALSGRSAAVASMKLSRVLNEAKSFYSATMAEPTDIYKMVGRPSNQQGRLVV
                     VDLQGLSDTAKQVICALLSSEILKAASSKTDPLRPCFIIYEEGHNFAPAGGNAVSHRI
                     IKKIAGEGRKFGVGFGIVSQRPSKLDSDVTSQCNTLITMRLKNPDDQRFIAKASDMVS
                     KADLEELPSLSTGEALVCGRSIPAPLLVKVGSKALIHGGESPEVLRVWGSFSG"
     gene            21032..21574
                     /locus_tag="ACHLAG_002027"
     CDS             21032..21574
                     /locus_tag="ACHLAG_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655266.1"
                     /translation="MPEVAWVNETFPLLASGKLLDQDVLVHSLGCSVWNTFGHEQSFM
                     AVVECPAPGTFGADIRSDSGWFHKSSASPVCLIEFERFDGSAKGQQKLEEKLKNLLEA
                     AQRWNHCPKTLVLSAWSQGLVGVPDTQKLKDICRMGFTSSTGTQVIAAPDVEVVFSRF
                     LFIKNLNMIVLDRIHYEVLM"
     gene            21571..23040
                     /locus_tag="ACHLAG_002028"
     CDS             21571..23040
                     /locus_tag="ACHLAG_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFP0655267.1"
                     /translation="MNIKSAHFLPGTNKRLGDKYLLLECLGDGSHGWVWRAERLKDGK
                     IVAVKIPKDITREDRQLAEGKELLNVEPHENIVQIFDMGRIDNEWFYIEMEYFPSQTL
                     AQKLDDRQRNFGQTYERLFRIYRQVLCAVHYLAELPVPISHGDIKPHNILVGERDLVK
                     LTDFGSSALPEEIYVRTRENGGTVLYSAPEFSNVDSRRGSLEELLLGDIYSLGVLLYQ
                     LLTGKLPHDTPAQVQRHAPFKLPTEINSSIHTDLEQVVLTCLQKRAEDRFSTISDLIH
                     AFDSAATKQLKVGAIAPVFQTKPAQDWSSAVLEAMSDQSYQKAAQLASQEYSRSKDLQ
                     ALHQQLIALYRANRLFDFEKVVEDNKAILLEGKLTQPAALFELIVKANLQLRNISQAN
                     FWLLQRKQAEAENAATMYLESSILGLEAKYSEARVLLEKVNQTTPMKFHVLSQLILVC
                     EQMRDYSGAAAYLKAALRVAPLDNSMKEKKELYAKLGVI"
     gene            complement(23241..24506)
                     /locus_tag="ACHLAG_002029"
     CDS             complement(23241..24506)
                     /locus_tag="ACHLAG_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0655268.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLVDARGRRDEARKLLANGVDPSENKKAVKVEHEQEVITFEVV
                     AREWHASNQKWSASHSARVLKSLEDNLFAAIGKRNIADLKTRDLLVPIKAVESSGRLE
                     VAARLQQHTTAIIRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLQRIY
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEVDFDTAMWTIPGEREPLEGVKHSQRG
                     SKMRTPHLVPLSRQALTILEKIKSMSGNRELIFVGDHDPRKPMSENTLNKALRVMGYD
                     TKTEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERMLM
                     LQWWADYLDANRMAAITPFDYAKINSSFR"
     gene            24868..26139
                     /locus_tag="ACHLAG_002030"
     CDS             24868..26139
                     /locus_tag="ACHLAG_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP0655269.1"
                     /translation="MSFKRDRYLISDSCGNYLVRIAIPKYMRHLFGGRCSYVKSTGTR
                     DIREARLFRDAVALEWIKLRNKLKPQREKTSKIEQIITELRKAGTYAFQVSESFNDSS
                     IGCPSLLNMRDQYILQFSEKRKLTTLSKTNKAVEVLLEHLNKRDVQLRDVNRTIVTSW
                     LDNLKNEKAPQTIQNYISALAQIWDLARNRYHDAPQDNPWRGHGLEAKSSKVNYEAFS
                     NDELAKVFSMLADEEEMQNITLIGMYTGMRINEIASLTIDDVKEIEGVLCFEITQGKT
                     KAAARVVPVHSLITPLVLSLREKPHNGFLFYHASITERADGKRSTWHTQRFTRAKRKA
                     LGEKGTERKVFHSLRHGVAQLLDRNQIPEDRIALLLGHTRGNTETFRTYSKNAASPVE
                     LKNYIELLRYPEIEKGLSINKKSNLRRKTAP"
     gene            complement(26219..26932)
                     /locus_tag="ACHLAG_002031"
     CDS             complement(26219..26932)
                     /locus_tag="ACHLAG_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFP0655270.1"
                     /translation="MATAGILTIRAANTPEQHVQAVYKAEQDINSTKNENSKANKTKG
                     ENGFAIVTSGLASSGNDVYENYMALAFDSVDDVNVRRSADVTSYPVENGATVSDHVQI
                     KNNKFSLKGRITETPIKSDPGLLKSAGVNGNRRSLAIDYLNQIMDSRQPFLLVTENKT
                     FENVVLTGIEYTEEASESLVFDLSFEQIRLVSYGTVNTVAIKTQPSKNIGANMKKRVN
                     TEKSSSEGEDTITPAFKQE"
     gene            complement(26964..28799)
                     /locus_tag="ACHLAG_002032"
     CDS             complement(26964..28799)
                     /locus_tag="ACHLAG_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655271.1"
                     /translation="MLEINTSKIKNTVTFSVDKDSLKKAKDSITGLKEFAENIKPAKL
                     RFDNVTKGYKKAQSEVDKIAQQKAKNEKQNQRAQAAAARAQARQQQQIAARKEKAELK
                     LLDVSSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKIN
                     GNRKAQLAPVKGGGSGNSATMGALLFGGATAAAGVMAVSKASDFVTSSFANAETQGEL
                     IQRAKLGGVDVNQMYNITEWAYRHGIDSLQGDQGARKYLDQMKDVRERAAKSYSEAEL
                     VKDKQTGKSKWKGGDNAINELLNIGVINKSDLKKFADNPAGLISKAVNGMMKKGFSDS
                     QIGQRLEDLGDDLMLTSKYWQRSVKDVQESINQQKASGKWLTETQQESIVKFRELNRQ
                     LSQLSDARQVAFVDGFMKSLDPKVTEEFMKNLSNLTPYFTKLGEASGSLFEAIMKIVN
                     WFNRNDDKTDAIQKNLGDAPPLSNEGMKQHLSNLVPDQYKGAGTATTAPDNSHSLFNT
                     IKGWLFDDKSSSVSDVKMSVSEAPITNLKQGALNNLAMTTPAYNLSPVFNLNPTFEVV
                     TEVPLTINSDTSRLSDYVDFTARASRDSFLKSLTLTSLSGQSNGG"
     gene            complement(28819..29016)
                     /locus_tag="ACHLAG_002033"
     CDS             complement(28819..29016)
                     /locus_tag="ACHLAG_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655272.1"
                     /translation="MEVILKSKNGVHVHLDANDSRGLLNLKYLCSLLDVPYEGVKARM
                     FRLNESIDQALHHFLSKEGGK"
     gene            complement(29104..30132)
                     /locus_tag="ACHLAG_002034"
     CDS             complement(29104..30132)
                     /locus_tag="ACHLAG_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP0655273.1"
                     /translation="MILGNDYVDLAPLFSAHSTRNYLLSTLDFTDPVGVSSHKVAVSQ
                     LVENNESLFNKETSRFSSEHNVTKREQGKEWLIEIPYFLREDVIRPSDVQGKRKPGTD
                     FQETLTDIYAEYVAKHHVAYQRTKESVLAASLFSGRTYTPKTDDVLIEWGKLFNVSAM
                     KATVNASSTDTTKIFKEFDQIATDIIEKAQSQAAAVERIVVFCKPEAFSAIRFSAGMA
                     NAFQYVSPLEEGNVVYQRRDLLPGVTAFTIPGTNIDVIKLVDPLHLAHMTADAVAVPK
                     FAKGAGVYANIFGAASSTFELINAAPAEVYSYSYESSRGDAVNVVTENSQMVVNHGVG
                     FSVQITVK"
     gene            complement(30151..30513)
                     /locus_tag="ACHLAG_002035"
     CDS             complement(30151..30513)
                     /locus_tag="ACHLAG_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655274.1"
                     /translation="MIIDLDTLFPVRKTFTDIVSYCTDPFASVENKVFASLPADVECG
                     DLITSTGAKYESGDDIYVVMSEFVTAGENKPVDVLRSNAGLVCIKADALNAVSEAAKT
                     ALIKKGFQLEGFHSVFTS"
BASE COUNT         8673 a         6839 c         7060 g         8084 t
ORIGIN      
        1 gcaaaaaata ctacacaaca taaatgctaa ttgtttatgc gggctttgta ttgctttctg
       61 tatcctacaa atgagtgaaa tttatgaaaa aggctaaaat actttctggc gtattattac
      121 tgtgcttttc gtcaccatta atttctcagg ctgcaacact ggacgtacgt ggtggatatc
      181 gtagtggaag ccacgcctat gagactcgac tcaaagtcag tgagggatgg caaaatggat
      241 ggtgggcaag catggaaagt aatacctgga ataccattca tgataataaa aaggaaaatg
      301 ccgcactcaa tgatgttcag gttgaagtta attacgcgat taaacttgat gatcaatgga
      361 cggtgcgccc gggaatgtta acgcatttta gcagcaacgg cacacgctac ggaccctacg
      421 taaaactgtc ctgggacgcg acaaaagatc ttaattttgg cattcgctat cgttacgact
      481 ggaaagctta ccgacaacaa gacttatccg gtgatatgtc tcgtgataac gttcatcgtt
      541 gggatggata tgtcacttac catattaata gtgatttcac cttcgcatgg caaacgacgc
      601 tatacagcaa acagaacgat tatcgctatg caaaccataa gaaatgggcg acggaaaatg
      661 catttgttct acaataccat atgacgcccg atattacgcc atacatagaa tatgactacc
      721 tggaccgtca gggtgtttac aacggcagag ataatttatc ggaaaacagt tatcgcattg
      781 gtgtgtcatt taaactgtag tagacaggag acagtcacaa tgaataaaac aataacggcg
      841 cttgctatca tgatggcttc atttgccgca aacgcgtctg tattacctga aactcctgtg
      901 ccatttaaaa gtggtaccgg agcaattgat aacgacactg tctacattgg tttaggtagc
      961 gcaggtacgg catggtacaa gctggataca caggccaaag ataaaaaatg gacagcgtta
     1021 gctgcattcc ctggtggacc aagagatcaa gcaacctcgg catttattga tggcaatctg
     1081 tatgtgtttg gcggcattgg caaaaatagc aagggcttga ctcaggtatt taatgacgta
     1141 cacaaataca accccaaaac caatagttgg gttaaattga tgtcgcatgc accgatgggc
     1201 atggcgggtc atgtgacttt tgtacacaac ggcaaggctt atgttactgg cggtgttaac
     1261 cagaatatct tcaatggcta ttttgaagat ctcaacgagg ctggaaaaga ttcagcaact
     1321 atagacaaga tcaatgccca ttattttgac aaaaaagcag aagattattt cttcaataag
     1381 tttctgttgt cttttgatcc ctcaacacag caatggagtt acgctggcga atctccctgg
     1441 tacggaacgg ctggtgcggc gcttgtgaat aaaggtgata aaacctggct tattaatggc
     1501 gaagccaaac caggattgcg aacggatgcc gtatttgaac ttgatttcac cggtaataat
     1561 ttaaaatgga ataagcttgc tcccgtctca tcaccagatg gcgtcgctgg cggttttgcg
     1621 gggataagca atgattctct tatatttgcc ggaggggccg gattcaaagg ttcacgagaa
     1681 aattaccaga acggtaagaa ctatgcgcat gaaggcctaa aaaaatcata tagcactgat
     1741 attcatcttt ggcataacgg gaaatgggat aaatcgggtg aattatcgca aggtcgggcc
     1801 tacggagtat cattgccctg gaataatagt ctattgatta ttggcggtga aactgcaggc
     1861 ggcaaagcgg tgacggattc agttttgatc tctgtgaagg ataataaagt cacagtacaa
     1921 aattaacgct tcagggcccc ggtaaagggg ccctgctatc aatttgtcat ttcaattacg
     1981 cgaaatttat atgaacgcaa taatatcgcc cgattattac tatgttctta ccgttgctgg
     2041 tcagtctaat gccatggcgt atggcgaagg actgccatta ccggacaggg aagatgcgcc
     2101 tcatcccaga attaaacaat tagcgagatt tgcgcatacg catcccggag gcccgtcatg
     2161 tcactttaac gacattattc cactgactca ctgcccacac gatgttcagg atatgcaggg
     2221 ttatcaccat cctctggcaa cgaatcatca aacacagtac ggcaccgttg gccaggcact
     2281 gcatattgca cggaaattac tgccctctat tcctgataat gcaggggttc tcatcgttcc
     2341 atgttgtcgt ggcggctcgg cttttatcgc gggcagcgaa gggacatatt cagaacggca
     2401 cggagccagc catgatgctt gtcgttgggg atcggatact ccgctatacc aggatttagt
     2461 cagcagaacg cgagccgcac tggcaaaaaa tccgcagaac aaattcctcg gcgtatgctg
     2521 gatgcaaggc gaatttgact taatgaccag tgactacgcg tcacaccctc aacactttaa
     2581 tcatatggtt gaagcctttc gtagggatct aaaacaatac cattctcagc ttaataatat
     2641 tactgacgcg ccgtggtttt gcggcgatac cacctggtac tggaaagaaa atttcccgca
     2701 ttcgtatgaa gctatttatg gcaattatca aaataatgtt ttagccaata ttattttcgt
     2761 cgacttccag caacaaggtg aaagaggact gacgaacgcg cctgatgaag acccggacga
     2821 tttaagcacg ggatattacg gttcagcgta ccggtcaccg gagaactgga cgacggcact
     2881 gcgaagcagt catttcagca cggcagcccg tcggggggtt atttctgaca ggtttgtaga
     2941 agcaattttg cagttttggc gcgaaaagtg agcgttcatt aatttgattt attcgcatcc
     3001 atcaacgctt ttaactcctc cggcgtaaaa ggtggtttgc gtcggtgcaa catcgcatgg
     3061 caattagggt acacaggcag caaaaaaaca aatgagatca aagtaatata tcttcacttg
     3121 caggcttgaa cagccgaaaa tttgcgagca tgatcgcaat tatttcactg ccagggatat
     3181 caatggcttc cagtaaatcg ctacagcagg caattgccaa cataaaaatc tggcacaaag
     3241 gtgaacagcg cgcgccgcat aagccattgt tattgctata cgtattagcg ggatacctga
     3301 atggacatcc acgccttttc gattatggat cggaaatcta cgaacctctg cacagtttac
     3361 tggaacgttt tgggccacag cgttcacatt accgacctga tatgccgttc tggcgattac
     3421 aaggcgacgg attctggcaa ttacacaatg ccgagctttg ctcaacagca gggagcagcc
     3481 gacaaccgcc agtgaaggaa ctgaacgaat accacgttgc aggcggtttc gatgaacagc
     3541 actacgccct ggtaaccggc aataaaaaac tcatcaatac tctggcgcaa cagatactcg
     3601 aagcgcactt caccgagagt attcaggaag aaattgctga cgagctgggt tttgatctcc
     3661 agcaaatccg taaacagcgc gaccctttat ttcgcaaaaa tgtattacgc gcttataact
     3721 accagtgcgc gatatgtggc tttaatatgc gccacgacga caccaccgta gcgttggaag
     3781 cggcgcatat aaaatggaaa cagcaaggag gtccttgtga aattcctaat ggcctggcgc
     3841 tttgcgcaat tcaccataaa gcctttgata aaggatcgat tggcctggat gaaaatatgc
     3901 gggtactggt atcagatgcg gtaaacggcg gagggatcgt tgagagatta ttctgggatt
     3961 ttgacgggaa aactattgcg ttgccgcagg tgcgtaaaaa ttacccttat gaggtgtttg
     4021 tagagtggca tcggaaagag gtttttaggg ggtgaggttg ataacagcat tcaccctaca
     4081 accttacatt gtatttaaat tagagctgag ttaacagata gttgtatata taacaggcac
     4141 ttgtcacaac gattaactca atatagttag aatacctcat attaattaaa aaataagtaa
     4201 tgtcacaaca gaggaaaagt cacgatgact cttgtagata gtgtcgaagc aggtaggcta
     4261 acgattagtg aattgattga tgccctggcg aaagataaaa attacacagc ttccagatgg
     4321 tatcagcgat accgtgcatt tacgactttg ctacagcaaa cctcaacttt tgctgagcct
     4381 gcaacagatg gtctggtcaa acagctttgg tatgagcgtg acaacggcat tgcaagtatt
     4441 cgccagggcg ttccatcctt agcagaatat cagcaaagcc tgccactgct tagagaacta
     4501 actgaacgaa ttcggcaaca gccggatgaa gaaacttacc aatatgttgg caatgcactt
     4561 caacaagcta aagaaaacgg acttctcaag cgtatgtatt ggagtttgag aaatcgcgtc
     4621 tttgccgcgt tctcgccaga aaactacacc agtactgtgg atgagaatgc ttttaataaa
     4681 gcagcagaat tcttaaatca gcacttccat ctcggtttgg tactgaccgg aaattggtta
     4741 cagaaaaact atgaattgaa acaagccata cacgcccaat ctcctgatac agatccttat
     4801 tatgtgaata tggccatctg gcatctctat gaattgctcc gtgaacgcga taatgaacaa
     4861 aagcaggaga aagtagctag cactacatcc ataacccgca gtgagcccat cgagaacaag
     4921 atcatcctac attcaccaac taacgtgatc ttctttggcc cccctggcac tggcaagacc
     4981 ttcaggttgc agcaaaaaat gaaagagtac acttctcatg ctgttcccgc tgatcgtgat
     5041 gcctggctgg attctcgcct tgaatcgttg aactggatgc aggttataac gctggtgctg
     5101 ctcgatcttg ggaaacgagc gaaagttcgc caaattattg aacatatgtg gtttcaacgt
     5161 aaggcattat taaacggtcg taatggcaat ctatcgaata ctgcctgggc agctttgcaa
     5221 tcctatacag ttcccgagtc gttaaccgtt gattataaga atcggcgtga gcctgccgta
     5281 tttaacaaaa cagataacag cgaatggttt ctagttgatt cacagctcga gcaagtggag
     5341 gatttggtag agctctacgc cgaacttaaa cgtggcccta aatctgccga agccatccag
     5401 cgttttgcgg tggttacgtt ccaccaatct tacggctatg aagaatttat tgaaggtata
     5461 cgcgctcgct ctgacgagag tggcaatatc tcttatccca ttgagccggg tatctttatg
     5521 cgcctttgcc aacgtgcgaa tgccgatcca ggacatcgct acgccatttt cattgatgag
     5581 atcaatcgcg gtaacatatc caagatcttt ggtgaactaa tctcactcat tgaagtagac
     5641 aagcgtgcag gcatgcccaa tgcgatgagc ctgcaactgg cttatagcgg tgatcacttc
     5701 agcgtacccg gcaatgtcga tatcatcgga gccatgaata cagcggaccg ttctttagct
     5761 ctgatggaca cggctttgcg ccgtcgcttt gactttgtcg aaatgatgcc tgatctctct
     5821 ttactgagtg aagctaaggt gaaaggcata gagctcgagt cgttgttaga gaaactcaat
     5881 agccgcatcg aggctcttta cgatcgtgaa catacgctgg ggcatgcgtt ctttatgccg
     5941 gtaaaaaatg cactcgatgc cggtgatgaa gaagctgcgt ttaaacaatt gaagatcgca
     6001 ttccagaaaa agatcattcc gcttttacag gaatactttt tcgatgactg gaacaagatc
     6061 cggttggtgc tggcagacaa tcaaaagcaa gacgacaacc tgcaattcgt gattgagaaa
     6121 accgacgatc tcgatacgct ttttggtaac aaccatggtt tacgacgcca tgatcagcaa
     6181 tcaacagctt atgagctcaa agatttcgat caagagatct ggaatattcc acaggcttat
     6241 cgttcaattt atcagcccca acagactccc cttgatgagc aggcagtaaa tcatgggtga
     6301 agttatctcc gtttttgaat atgacctctt ggggagtgga aaagcggctt cagttggcgc
     6361 aaaaccggtg cctccacagg tctttaatta tttagaagcg cttagcctgg caagtaatca
     6421 agggagccag ttcctcaaac tcacctctcg ctcaggcttc aaattgcttc aggttcaaaa
     6481 ctacgcaggg atgctttcaa cacctcacgg tttccagctt gagatactgc ccaaggttgg
     6541 taaaaacctc acagcggcta atgcacgtca aacattgctg acgatgctaa gccatctgcc
     6601 tgggttccga catattcaaa cccagcaagc cactcttcag gctcagcgta tgcctttgct
     6661 tgagattttt attaaccagt ttttgcatag cgtcagccaa ttgcttaaac agggtttacg
     6721 ctccgactat gtgagcaagc aaggtaatct ggcttttatg aagggcaagc tgatgctttc
     6781 tgcacagctg cgacataacg cggtgaatcg ccataagttt tgtgtcgatt atgatgacta
     6841 catgcctgat tgtgcagcca atcggctctt acactccgca ctagataagc ttcttagtct
     6901 gcagctgtca tcagataatc aacgctggct ttacgaactg cgttttgcgt ttgacggtat
     6961 tccacttagc cgggatattg aacgtgatat aaacaactta cgcctcgagc gtggtatggc
     7021 tcattacaac gagccaatgg catgggcgca attgatcctg aggggaatga gcccgagtgc
     7081 tttgcaggga aacaccaaag cgatatcact tttgttccct atggaagcgg tatttgaatc
     7141 ctttgtggca cagaccctac ccgacgagtt accacctcac ctcaaagttt tacctcaagc
     7201 agcaatttat tctttggtaa agcatggagt taaagattgc tttaagcttc gccccgactt
     7261 gctgattcaa tcaaataaac cagttcaaac cgaaatggtg atggatacaa aatggaagct
     7321 ggtgaatagc agccagcaga caaaatcact ctatggactg gcacaagctg acttctatca
     7381 aatgtttgcc tacggacaaa aatatcttgg tgggaatggc gaaatgtatc tgatttaccc
     7441 tgcgcatgat gactttagcc aaccgatacc gcagcatttt gctttctcgg agactttaaa
     7501 attatgggtt gtgccattcc ggataatggc aaaacgcggt gagaggatga tgtggccgaa
     7561 tgacgtgtcc tgtacaatga gcccccaact ggatcggatg gctgcttcag tatcctatag
     7621 tggaggcatg agatgaccca tgaataccag cgacaaagcc tggaacaact aataactcag
     7681 caaacagcaa ctcccagaag cgccgccgtt gtatttaatg agaaccaaac cctctggcaa
     7741 acacttggct ttagccagtc tcaagtttcg ctatggcttg cctcattgcc tcaaagtaat
     7801 catctaaaag ggcccacaac ggcaacgtac caggttatac ctgatatcgc ggcacatttg
     7861 gtatcattgt tgcatcaagc tggtgggcgc atgccccttg cacaagtatt gaaaaagctc
     7921 cctgctggta ttacgaccag tgaacagcag atacgcaaac tggcacagaa gcacgcgcag
     7981 atagagatta aaggcccctt gctggtactg ataaattagc cggcatttgt agtaaccatt
     8041 ggctgtatta aaggatttta gaaaacatca tggcaaaaga agcaaaaaaa caagacccgc
     8101 aacagcagct agaagcactg ttcaataaac tggtcaaaca gcactccgag tttactcagc
     8161 cattggtcga attacccgag catgaattcg ccaaggctgg tctcattcaa ggcctggttg
     8221 aaggcaactt aacgccacca gtcatgttga ttgcgctgac agaaggccat tgggaatacg
     8281 ccgctgacac tgcaaagttt gatgatttag cctatcagct gttaagtgat atcgaaggtg
     8341 actggccagt atatgccgtt gtggacgatg gcttgaacca ccgtattttg gccttgttcg
     8401 gtgccgatgg atctgacggt acccacggtg cagataccct gcctggtctg gaagaattac
     8461 gtagctttga tcgcatggag cgtgatccaa cctttcgctg gtcgatgcgt gtttatacac
     8521 gcttaatgca gcgatttgat gccttccatg aaaatgttta tcgcgttacc aaagacaagg
     8581 taaacgacaa gaacgacatt attgaggaag tagctaagct tctgttttta gaaactttcc
     8641 gcctgcatca cgatgaagac cttaccttta aagatgatga gggaaatacc ctgcgttttc
     8701 gtgatgtctt taattggcaa tatgtggaga gccacggcga taaagccgtt gcccaaatca
     8761 aagccgcctt tgaggaattt aaaagccacg aaaactacgt tgtgatctca gatgatggct
     8821 cacgtaaccc catctttagt aaagaaacac acctgcggct gtctgttgca aaaaactatc
     8881 aggatttgct ggaagcaata caaaacctgg gaccggtaaa aaccaacgac ggtaaaatcg
     8941 ctaaagaaca cggcacgttg gctgatgtgt caggagattt actcggcaga gtgtttgatg
     9001 tattcctacg cgctaacttt gaatccaaag gtggccttgg cgtttattta acacctaacc
     9061 cagtaaaaca ggccatgcta gaaattgcta tgcacgacat cgacgatgac gatgaaatgc
     9121 gatcgcgctt agccaatggc gattttcgtt tctgcgaccc cacctgtggc tcctttggtt
     9181 ttggctcggt ggcgttaagc cagattgata aatggatcga ttttaagctg gtgctggccg
     9241 acgataaaaa agaatcattg aaacaaaaat tacgcgactg tgcatttacc ggtgccgatg
     9301 ctgcaccacg catggtaatg ctggcccgcg tgaatatggc attgcaaggt gcccccaaag
     9361 cacagatttt ctataccgac aactcactca ccactaacgc cctgaagccg aatagctttg
     9421 accttatttg caccaacccg ccgttcggta cgcctaaatt tgataaaggc aaaaatggtc
     9481 aaaactcaaa agccaattac gaagccaata tggagcaagt gctcggtggt tttcgtccga
     9541 ccaaaaaagt cgttgatagc tacaacgcct ggtatgacca cgtaaaaatg aagtgggagg
     9601 atatcggtga tttagagctg gatgaaaacg gcgagcctaa atgggcgggc tatcgtaccg
     9661 atttgcgcag aacgggcggg actgataaaa agccgtttta cagcctgcag cctacaaccg
     9721 cagggctcgc gctgggcagc aagcccgata gcaagggcaa ctggcaaccg gtaggtgcca
     9781 ctattgaccc agcagtactt tttatcgacc gctgcctgca actgcttaaa cccggaggcc
     9841 gtctgttaat agtgctgccc gatggtattt tatgtaactc cggcgaccgt tatgtacgcg
     9901 agtacatcat ggggaaaaaa gacgaaaaaa ccggcgagtt tgttggcggt aaagccattg
     9961 taaaagcagt aatttcattg ccgagcgact gctttaagct ctctggcacg ggcgccaaaa
    10021 cctcgatttt gtatttgcaa aaacgccatg ctaaccccaa ccagccagag caatttttgc
    10081 ccgagccaca aactgacgtg tttatggcag tggctgaaac cttgggctac gtggtgaaaa
    10141 ataatattga agactataac gctggtgtcg caaacgatct ggataagatt gtatcggctt
    10201 ataagcgggg tgagtgatgg acaaattttt tcgaacaaac agctcaattc ttgtggaaag
    10261 acttgatcct aacttttatc aaccgagcct agtagaaaat gacaaactga taaaatccaa
    10321 aggctgggtg gaactccagt cactttatga agcgagtggt attggaaaca cgtcagcagt
    10381 cgaacggtat tactctgaag ctgctgatag tattccatat atttcgggaa aggtaattaa
    10441 aagcttcaat attgatttag atgaatgcca acgaatatct ctcgattcac ataaaaatga
    10501 gcttacaaaa tcagcgctca aaccaacaga tgttttagtt attagaaaag gagatatggg
    10561 aaatgcgtgc gtagttccat ctgaagttaa cgaagcaaac tgttcttcag aagtcatata
    10621 cctgaaaatg aaggcctcta gcgatcctta ctatctcgtt agctacctga actgtgacca
    10681 aggacaaaag gcttttaagc gactaggccg ggggactatt attcctggag tgagcttgtt
    10741 agatgttcca cgtttaccta ttccgaaagt gtctgagttt gttcaaaaat acatcgggga
    10801 taaagttcgc caagccgagc agctgcgcgc ttgggcgaag ctgcttcgca catcagttga
    10861 cgctcatctt aatagtctaa atctgccgat caacgagccc cccgcactat taaaccgagt
    10921 cagcgcgcaa acaatggagg atcgactcga tccacgtcct tatcggactc actacctatg
    10981 tttagtgcgt gaaattgaaa aactacctca tgacagcatt agcactttgg ttgaattggc
    11041 atctggttgc ccagtatcca gtaacgactt tttggaaaac tcaggcatac ctttagttcg
    11101 catccgcaat atcggttttg atgattttat tggtttagat actggcgtta gccaagatgt
    11161 atatcaggat gcgactaagt atcaagctaa agacaaaatg atcgttgtcg gtatggacgg
    11221 tattttccgc tcgcagtttt tcatatctga cgagctacct atgctggtta atcaacgggt
    11281 tgcaatgctc tcacctcaaa atattcgtgg cgaactatta acacattggc taaatcgccc
    11341 cgaaggacaa atgcagctaa atcaatgggc agttaaaaca actgtagaac atacttccct
    11401 atctgatatt ggtcgcgtac taattcctcg actggataaa agcttagaaa acaagcttgc
    11461 tgattatttg ctcaatgcga ggctggctta tcgttatgcc aaatttttaa cacaagtagc
    11521 caaaaccctc gtcgaagccc tcatcgaagg tcaactgacc gagcaacaat taatccaggc
    11581 tcaacaagcc ctggaagatg gcgacaacag ctttgatcaa gcgattttaa gtaaactgag
    11641 cgctgagggt tatgccatag aaggcgccac accgctattt agcgatgtgg atgagttata
    11701 cagtttgttg gaagaggcgg cgcaagcgga ggctgaagag taaagatgga gacaaggggc
    11761 atggacagca ttacaaccag gcaaaataac gaccccgtaa atagcgaagc ggcacttaac
    11821 ctctgcctgc agctttggca tcaaggcggt ttaaccgcca gtaaagcagc gttgttactg
    11881 gcggctgtgc ctgccctcag gtcactgtta caaccaataa tacagccgaa aaagaaagac
    11941 gctgaaacgg atatcattag cgcctttagc ctcactgcac cattacttga tgcctttagt
    12001 gatttaagcc agtcgggtga atggcaatta gcgttgttgg gcttaaaccc agatgttcgc
    12061 caacattgga taaatttagc cgccgctcgc tgccaggaag ctggcgcgat gagtgaccca
    12121 atggtattgg ttaaactcat tcagcaactg ggcaatgcca gtgaatgggt cttagcccag
    12181 ttggaaaacg cagacttctc accacaaatc attgcaagcc cattggcgca aaccgaacgg
    12241 gatttactcg gtcatagcct aaacgacaat gctgccattc ccgctctatg ccgtattttg
    12301 cgcaccagcc ataccctgtt taccgtgagc gaacaaaatg aaccaccggc atctatacaa
    12361 gcggtggatg tcaccgcaaa acaactgaca aataactggt gtagtgggcg gttgttagca
    12421 ctccctaata ccttgcttga tgaacataac cttaaaccca atgccgattg gttattggtg
    12481 tcccgctcgg gtcatgacaa cgtgccgctt accgagctat tcgcccaaca gccctggtta
    12541 tttttactca gtctaattat ctttgtgcaa gatgcctggg ctgccgagca acgtggcggt
    12601 ttgttgctca ccttacctgc cgggcaaaat gcctttgccc ccggtcaaat aaatgtggcg
    12661 gtacagggca ttgaaggtga tgaggtgagt ctgggtagtt tggccgagtt tcttgtgctt
    12721 ctgctcggtg agctcaatat ccccctctat ccggcactcg atgcgaacac ggaaagcatc
    12781 aaccgcctaa accgcgtcct gagctcattc attgccgaat tgttggcaaa aaaaatctgg
    12841 caatttaccg aggcgggtcg tggtgagtcc ggccaatatc gaattcatac aagcttcagc
    12901 gatgcctgtt atagcctgcc gttagcacca ctatttggct acaaaagcca aacgttacaa
    12961 cgggccatta agcaactggc gcaaaactgc tatgccaata aaaaacgcgc cgccaataga
    13021 ataaaccttc aaggatcgtc gttatgagtt ttgaattaaa cgaacagatg gttgatagcc
    13081 tggcaaaaca acatcgctgg gcggcgacgg gcgtatcaga ttatccacct cgcgacccgt
    13141 tagtgggcca gagccgcttt tttaaacgct ttcaaacctt tttgcatacg gtggatcacg
    13201 acgatgatcg ctttgcccat gtgtttgccg tcgaagccga atggggtcgg ggtaaatcac
    13261 gtttaggcca tgaattggtc gcacaaatta acgactgttc aaggggctgg tttgtccgcg
    13321 acgaacaagg ccaactgcac gataagcagc tgtttaatcg cgacacgcaa gacaaatacc
    13381 tggcgctcta cattcgctac tcgcaagtgg catcagatta tcaaaactcg gataactggt
    13441 ttgcttttgg tttatatcag gcgctattac cactggccac ccaaaccttt gatggatcga
    13501 ttcaaagtga aattgccaaa caagcctatg aacgcttaat gccatttggc tttaaatctg
    13561 aactgttagc agatgcctta cagctgaacg ccaaccacag cgaagaagac ctctatactg
    13621 accaaacgct ggtagtcagt ttggtgcaag ccgcctataa agtattaaag agctttggcg
    13681 tgcagtatat gttggtggtg ctggatgagc tggaaaccgt tgcagaagcc gccacctttg
    13741 gcctggaaga tgaacaaaat aaacgcctcg atggccaggc cattcgcctg attggtaaag
    13801 caattaaaga ggaagaccct cgccgtaaac ttccatggtt gcgctatgta gcgctgtgtt
    13861 caccactgct tggccagcaa ttgcgcgaaa ttcaatcggt cgcacgccgc tttgagttag
    13921 tggaacttga gcataatgcc tttgccgatg tatcagacta tgtggcaagc ctcttaaaag
    13981 acggcaagct gggttttaat taccctgctg gtttagtgga agctgcatac gccatgagtg
    14041 gtgccaactt cggttggttt aacgtggtca tggctaatgt ggatgcagtg ctgagccaat
    14101 accaacaagc aggccgcagt atcaaggatg tgggcgattt atttgaagct gtgctggcag
    14161 gctctggccg cgtagccagg catgtgctgg ataaaggcgc gatagaaggc attcaaactc
    14221 gcgaccaggc actattgacc gaatgtcgta gtttgctcta cggccaatta cccgtgcgct
    14281 tagctgaaat ggcctatgcc acagaattgc tggcgctgaa aaacgaagat ctggaaccca
    14341 tagccgctca ttaccgcaaa ctgagcattg accgcctaca gtgccgacaa gccctggagc
    14401 aagccaaatt ccgccgcgat aaagatgaat ggtactaccc tgcagtagat caagcgctga
    14461 acttagatac tttgctggat aacctgcgta ctttttcaat taaagaacaa gccgcaagcg
    14521 gcgataaagg cgcggtatta ctacctctgt caaaaggtga gtttaaatac ttactcagct
    14581 tgctgtatga ccacccagcg attgaattcg ctgccgatgc cttatggcac aagctagtgg
    14641 gtgaaactac cgaactggac gccagcgaag caacgcatat tggccccagt gtggcgatgt
    14701 tactgcgcct ggatttgcgc tatcgcagtg cccagcaaaa ctcactgatt tttaaagatc
    14761 ccgccatggg cgacgctcat gaacaggcaa tgagtgaact tagcaaacaa agtagccaga
    14821 agccgctcat caaggcatta gcccgtgtaa cgggctttgt gagattactt gataacaact
    14881 ggacctatga tgaaaacttg ctgagtaata aagcgggcag cgacgatgca cctttggcca
    14941 ttttaaccga gccccgtggc cgccagggtg gtttacttac cctggatgga tttaagcttc
    15001 acccagaagg taaagccttg tttgcctggg tcagcaacct aattgagctg aacaatcttc
    15061 acacctttgc ggcaaaccac agccaccaaa atggtcgcac gccagtatta gcgttaactg
    15121 cttctgcaca tctcatggag caatacagcc gactggatga aagaaatgag ttgcgtgatg
    15181 cgatactgct gcattatgtc aacccaagcg aagcagacca gttagagcgt attggcttaa
    15241 gtttggcagc atgtcagttg cacggcgtga atttaactgc cgacagcttt accgcgaaat
    15301 tcaaaaacaa gctgcatgcc ctgacaacct ttgccacgga tgccattcac aagtggcgcc
    15361 agcgtttgca gcagcgcggt ttaattgcct ggccgttaaa ggtagatggt aaattatcac
    15421 cgaatgaccg agacctgttg tttaaaggct ggtatcagct ggccattgct caccctgagt
    15481 taaatggcat tctcgattta cagcagcaac acggcgtgcc agtgaatgag ctgagcagct
    15541 tgttggataa acttaaggtg ccgggcagct atattgccaa aggttacact gccgatgaac
    15601 atgccgggct atttactgaa cttaataatg tgcagcgaag ccaggcgcag atcccattat
    15661 ttttagcccg tattgctcac cctaataaaa agcacaagtg gcaattcgaa ggctttaagc
    15721 aacagtttta tttcgcctat gtggctgaaa cgagtgttac cgccaaaggc gtgtttaatg
    15781 actggatgtg gtggtgtgga gagctgaact tactcacttt aaccaatcca acagaaaaac
    15841 aggcaatttg ggagcactac ccgcgctccc gcttagaaaa tgccattaga gaagcgcaaa
    15901 actggttccg tggtaacgat atgggcagct atgcagccaa tgtggaagtc atgagccgcg
    15961 tgtatggtta tgcacgcatt aatgaaatgt tcgcgccact gggtaaaaat aagctgggtt
    16021 tcgtcaccgt ggaagctaaa gagcagctgg aaaaagcaca atcgctattt aacgtgctca
    16081 aacagcaaga agaacaattg gctgatatgg tcgaggccaa cgacacgaaa gtattggcag
    16141 ggcttatcca caaacgcgct gaagtgctgg agctggtagc aaaggttaaa ccgcttaata
    16201 gttccaggcc tatgctaaaa gatgcccata ttctcagctt agaagacaaa acgacctcgc
    16261 tttatcaacg gattgaacag gcctgtttat ttgccgagtt tgttgagtgc agcgctgagc
    16321 gtatcaacaa ccgactcgtt gatttaatca tagatgttga aacagagtgt gccccgctga
    16381 cgaattttcc gaaacgtctg tacaccaaca cgctgcgcac cataggtcat attttagatg
    16441 gagcgttaaa agacgatacc agcagtgcca ctggccgaaa agagcagcaa gcagatagcg
    16501 ataccttact gcattacctg cgtaaactcg atttaggtag agcgcatgac aagctttcag
    16561 ccttagcgca agaagtgggt ttaaacctgc acaatgatca acagttgccg atagcggaga
    16621 ttcaggggca tattttgtcc agctaccgca actgcaaaga acgatttagt aagctggtta
    16681 ataacctcac agagcaaaaa ctacgtgcac aacaactgca agaatggctt agtagcgcca
    16741 cggctgagta tcagttcgcc gatgacattg cagagctacc aaaactggta atgaaattac
    16801 agctcattga agatgctatt gccgatctcc ccagtgatgt agagagcaag cgtcaaagta
    16861 tgcaaaacag tttgcgcaat gggcagtttt catcgttacg ggatttacca gaagagcttt
    16921 taaaaccggc acgcggtcaa cttacaccga ttcaaggcca gctacttaaa attgaagagc
    16981 gtctaaacca agtaagacgc aactccatcg cacaggtaaa cagctggctg ccgttattaa
    17041 aaccgctatt ggcttctcaa aagcaagcgg aaccagcagc gttgactctg gaggatgtga
    17101 gcaatttggg tgtcagagaa cttcaacagg tgtgtgaaag cacccaggcc aaatggcaga
    17161 gtcaaggtga gcaaatatta aatgatacag gcttaacact ggcggattgg caaccgattt
    17221 ataaggcttt gagccaaaat caggaacccg tgttaacacc tgagcaacag aaaggacttg
    17281 tagacaaagg cattgtaaaa atgcgcctta cctttgccac tggtttgtag aggtgacgcg
    17341 atgagtcgtt atttatctgc ggcactggcc agtaatcgta aaggccgttt tttgcaaact
    17401 gttgcggggg ctaccccctt aatgaaggat tggattagct cgctgccagc cagcggtttg
    17461 ttaatagtgc aagcagaaga gttgactgac gctaatacta tgcagcacct gtatcactgg
    17521 gctatgcagg caggttgtgc cgctttggta ataaacctga aagcagaaca atttacctta
    17581 ttagcgcaat tgccctatcc actggattgg cagttagtac cagcgagctt gagggggcag
    17641 gaaccggggc taaccgcttt gctagccagt gaaacagacc aggctattgc tggctttact
    17701 ggcagcgcag atcgctacca gcaccaggcc ggtgatgtag tgcatacccg ctacatacga
    17761 aaacacagca acagtgggct attagccttt accacgttac ctttgtggtc gctgacttta
    17821 ctcgatcata gtgagctcct ggtgagctgg ttgaattggt ttgtggacca tgcaggcatt
    17881 gctgaacgaa taatcgaacc taaagcgcca tcaactgact acacgccaga taagcacgat
    17941 ctggttgtat tgctattact ctacgctggc ggtggcatga acttacaagc gttgtctgag
    18001 cataatgcgg ttaagctgat gtttgatgtt aatagccttg atattgtcaa gcggggtgag
    18061 atgttgcggc agcacgattt tatcgacgac gcgggcataa cagctaccgg taaaacatgc
    18121 ctacaagcct cgcaatattg ggcttatgcc ccattacttg gcgagcagct acacacagga
    18181 acgctataag catgaatgag aacaaaacga tgaatttagc gcaatcgctc gccatcgata
    18241 gccagcttcg cttaccggga agtgcaggac gcttgcttaa acaggtccgt gatattgagc
    18301 aagtggcgcc gctattccgt gatgctggct tagtgaaagc ggtccccaag cccacaattg
    18361 ttaatttacg cgtagccggt attgcgacta aatcgtgctt agacaaggta ttgggtggtt
    18421 ttatttatgc cagtgcagca gtgcgcacag atttgttaat agatgacaat aaagtcagca
    18481 cggtgggtag cgactcggta tcagaactgg atgatattga ttttgaagct cagcgtaaac
    18541 ggcttgattt aattgaaatt cgtcaggctt atcagttagt tgagaaaaaa cttttaggtt
    18601 atgagcctgg cgatttaatt ttgctggata cgccgttgtt tttagcacgg gatatggctc
    18661 cgctggagag aaatgtccgt cataaacaag agtacgaaaa aaccagacaa gtgatagaaa
    18721 ctttttggca aaaccatcgc tccagaattt ttccctggaa tcaaaatggt gtagtacttg
    18781 cgagcatttt ggctgagcgc tttagtgcca ttgtaagtat cgccaaacaa gatttacgta
    18841 ccgaacaagg acgcaaacaa attttagcat ctgatgggtt ttctaaagag ttgatgcaaa
    18901 atctggaagg actggatgaa agtttggttg gcgtgggaga tttgcgattt atcaatggca
    18961 ttttaggcaa ctattcgcgc accatcgcat ttcgtttgtc tgaacaagaa aagcgcattg
    19021 aacccaatat tgaagctaag caaggtctaa ttggttttca cttccgtagt gcccgaggtg
    19081 gacagataaa aatggtgcaa ttagcgggtg acgaaccaga atggaaaagt gctgatctcg
    19141 atttagttgc ctctcgcctg atggtgctgg atatgcaaaa caaaggtaat gcaatgccgt
    19201 tgccacaatt acttggttat caacagcttg gtattttacc taaatttgct acgttttatc
    19261 gccagggttt acaccaggca ctaaagaata atgatattga ggcagggtgg ttggcaggcc
    19321 tcgatgaggg attgaataat ggcttataaa gttttaggta aattagtcgg taacactggt
    19381 gatgccagtc agttaactat ggttgtacaa gactcgtttt cagtacgccg tggtgagttt
    19441 gtacgcatta tgcaccagga acgcaaagat gaggggcaag tcgccgtact ggggcgagtg
    19501 accaagataa gccgtactaa tatgctctat aacgccggct ttggcgatgg tgtaaccgag
    19561 cttgagcttt tgcccggtgc gaatgtaact ggtgagaata tgtttgctca ggtcgagctg
    19621 gttggttacc gcgatcctgt tactcgtcaa atcaaaattc cacgtcgccc gcttaacccg
    19681 ggtaccgctg ttgaaaccgt cgattttcag tttttgagtg atttttacga atttaatgaa
    19741 cactccagtt tgcacttagg taacttagta ggctacgaca aaggtgagaa tacagttcct
    19801 gtttttattg acgtgaacaa gttggtcact gagcatttgg ctgttctggc aatgacgggt
    19861 tcaggtaagt cttatacagt tggccgcatt attgagcgtt tagttgcggt taataacggt
    19921 acggtagtag tatttgaccc acatggtgaa tacggtaaag ccctcgctgg cggtaatctg
    19981 caattctcaa gcttcttaga agccactgac gataagcgtg atcaagaagc actgccactg
    20041 ataaaacaga cctttgaaaa gctgcaagcc gccggtgcag gtattcaggt ttactcgccg
    20101 cagcatgaat cttttaaaca taaatacgcc agtaagaata cgccattagc gcttcagttt
    20161 gaccattttg aaatggacga tattgctgaa atattacctg gcttaacgga accgcagcaa
    20221 cgagtactcg atgttgcaat tcgttattgg cgctatgcag ataagactga gccacgagat
    20281 attaaccgcc tacgcttctt attaggtgat ggtattgaag agttaaaaga gtgggacgac
    20341 ctttctgaag ctgaggcgaa ggcgttatca ggccgtagtg ccgccgtcgc ctcgatgaag
    20401 ctttcacgtg tgcttaatga agctaaaagt ttttacagcg caacaatggc tgagccaact
    20461 gatatttaca aaatggtggg tcgcccgagc aatcaacagg ggcgtttagt cgttgttgac
    20521 ttacaaggtt tgagtgatac cgctaagcaa gtcatttgtg cactgttatc tagtgaaata
    20581 ttaaaagcag cctcgagcaa aacggacccg ctgcgcccat gctttatcat ttacgaagag
    20641 ggtcataatt ttgccccggc cggcggcaac gcagtcagtc accgcattat taaaaagatc
    20701 gctggtgaag gtcgtaaatt tggtgttgga tttggcattg tgagccagcg tccatcgaaa
    20761 ttagattcag atgtgacctc tcagtgcaat acgctaatta cgatgcgctt aaagaaccct
    20821 gacgatcaac gatttatagc taaagcatca gatatggtga gcaaagctga tctggaggaa
    20881 ctcccgagtt tatcaaccgg tgaagctttg gtgtgtggtc gttcaatccc tgcaccactg
    20941 cttgttaaag ttggtagtaa ggcattaatt cacggtggtg aatcaccaga ggtactgcgt
    21001 gtttggggct ctttcagtgg ctaaggtgcc tatgcctgaa gttgcctggg taaatgagac
    21061 atttccactt ttagcaagcg gtaagctatt ggaccaggat gtattggtcc acagcctggg
    21121 ctgtagcgtt tggaatacat tcgggcatga acagtcattt atggcggtag tggaatgccc
    21181 ggctccaggg acatttggtg ctgatattcg ctctgactcg ggctggtttc ataaatcttc
    21241 tgcatcgcct gtgtgtttaa tcgaatttga acgctttgat ggttcagcga aggggcagca
    21301 aaagctggaa gaaaaattaa aaaacttact ggaagcagca cagcgctgga atcattgccc
    21361 caaaaccctg gtcctcagtg cctggagcca gggcttagta ggtgtacctg atactcaaaa
    21421 actgaaagac atatgccgta tgggttttac ttcgtccacg ggtacccagg taatcgccgc
    21481 tcctgatgtt gaggtggtat ttagtcgctt tttatttatc aaaaacctga acatgattgt
    21541 tttggacaga attcactatg aggtgttgat gtgaatataa aatcagcaca ttttttgcca
    21601 gggacgaata aacgccttgg tgacaaatac ctgttacttg aatgccttgg tgatggcagc
    21661 catggctggg tatggcgggc ggaacgcctg aaagatggaa aaattgtcgc agtaaaaatc
    21721 cctaaggata ttactcggga agaccgtcag cttgccgaag gtaaagagct tttaaacgtt
    21781 gagccgcacg aaaatattgt gcaaattttt gatatgggcc gtatagataa tgaatggttc
    21841 tatattgaaa tggagtattt tcccagccaa accctggcgc aaaagctcga tgacaggcag
    21901 cgtaattttg gccagaccta tgaacggttg ttcagaatat atcgtcaagt gctctgtgct
    21961 gtgcattatt tggccgaatt accagtacca atatctcatg gagatattaa gccacataac
    22021 attttggttg gagagcggga cctggtgaag ttgacggact ttggcagctc agctttacca
    22081 gaagaaattt acgttcgtac ccgtgaaaat ggcgggactg tgctgtattc ggccccagag
    22141 ttctccaacg tcgatagccg tagaggcagc ctggaagagc tactattggg cgacatctat
    22201 agcttaggtg ttttgctata ccagctgctg acaggaaagc tgccccatga tacgccagca
    22261 caagttcagc gccacgcacc tttcaagctt ccgacagaga ttaatagctc tattcatacg
    22321 gacctggaac aagtggttct tacttgttta caaaaacgag cggaggatcg cttttccact
    22381 atttccgact taattcatgc atttgattct gctgccacta agcaattaaa agttggtgct
    22441 attgctcccg tttttcaaac taaacctgct caggactggt caagtgcggt acttgaagca
    22501 atgtccgatc aatcttacca aaaagctgct caattagcgt cgcaggagta tagccgcagt
    22561 aaagatctac aagctttaca tcagcaactt attgccttgt atcgcgccaa tcgcttattc
    22621 gactttgaaa aagttgtaga agataataaa gcgatcttac ttgagggtaa attgactcaa
    22681 ccagcagctt tgttcgagtt gatagttaag gcaaacctac agttacgcaa tatcagtcaa
    22741 gcaaatttct ggctgttgca acgaaagcaa gctgaagcag aaaacgcggc caccatgtat
    22801 ttagaatcat ctatccttgg ccttgaagcg aaatattctg aagcaagagt actattggag
    22861 aaagtaaacc aaacaactcc aatgaagttc catgtgttaa gccaactcat tttagtttgc
    22921 gagcaaatgc gtgattactc tggtgcagcg gcttatttga aagcggcatt gcgagtagca
    22981 ccattggata acagcatgaa agagaaaaaa gaattgtatg caaagcttgg agttatttga
    23041 ttgaaaggat gaccattgcg cgaacaaggc atagtggata tttaacactt ccgttcacat
    23101 accggacaaa aaaataaagt tttacataac gatttccttc ttgattgagt taagacaaaa
    23161 gcctctcccc attaggacgg aggcttctca gttataagcc tttaattaaa aaaagttaat
    23221 attcatatac ttagctagaa ttatcgaaaa gaactattaa tctttgcata gtcaaatgga
    23281 gtgatagccg ccattctatt cgcatccaga taatcagccc accactgcag catcaacatt
    23341 cgctccccaa gatgttccgc cttatggata taagccgccc gtactgagct acgttcctga
    23401 tgactcatct gccgctctac tgcatccctc gaccacaatc ccgattcaat caaagaacta
    23461 cacgccatcg tcctgaaacc atgtccacac acttccgttt tcgtgtcata gcccatcact
    23521 cgcaaggcct tgttaagcgt attctcactc attggcttac gcggatcgtg atcaccaacg
    23581 aagatcagct cacggtttcc actcatgctt ttgatttttt ccagaatggt gagagcttgt
    23641 cgagataagg gtacaagatg tggagtccgc atcttcgaac cacgctgaga atgcttaact
    23701 ccctccagtg gctcacgttc tcccggaatc gtccacatgg cagtatcaaa atcaacttct
    23761 gaccagcgag caaaacgcaa ctcgcttgaa cggatgaaaa ctaacaatgt gagttcaacc
    23821 gccagtcggg ttaacggcct gccagaatag tgatagatgc gttgaagcaa ttcaggaatg
    23881 cggttaagtt ccagagcagc acgatgctgt cttttcgccg tagcaaccgc accagcaatc
    23941 tcttgagcgg ggttgtagtc gattaatcca ctctgtacag caaagcgtat aatcgcggta
    24001 gtatgctgct gcaaacgagc agcgacttcg agacgcccag acgactccac cgctttgatg
    24061 gggacaagca gatcccgagt cttcagatca gcaatgttcc gcttaccgat agcagcaaag
    24121 agattatctt cgaggctttt caatacacga gcgctatgcg atgcagacca cttctgattg
    24181 ctggcgtgcc actctctggc taccacttca aatgttatca cctcttgctc atgctctacc
    24241 ttaacagctt tcttgttctc actaggatcg acgccgttag ctaacaactt acgggcttca
    24301 tcccggcgac ctctggcatc gaccagcgag acttcagggt atttccccag cgccagcatc
    24361 ttctccttac cgccaaaacg ataacgaagt cgccagtatt tggagccatt agggtgaacc
    24421 aacaaaacca tgccttcacc gtcagtaagt ttataggctt ttgcttccgg cttagccgaa
    24481 cggaccttca catcactcag agccatgatg agtatccttt caagggccct gtgtgggtac
    24541 aaacataatc gaaccgggaa atacccgcag ttgtacccgc atcagtaagt tgatgtagat
    24601 tgaatcaggt tgacttaggt tgagtgaaaa agcgaggaaa gccttgcgga tactggattt
    24661 caggcataaa aaaagacctc agttgaggtc tatttacata cttttggtgc cgaaggccgg
    24721 actcgaaccg gcacgatttt aacggtcgtt cgcttgccta gcaagctcct gtaatgcttt
    24781 gaatcaaaag caaaactaac aacacatcga cacttcacac tgtacactat caagtgcaaa
    24841 agccctgaca aaatagccgg ggtttgtatg agtttcaaga gagatcgtta tttaatttca
    24901 gattcttgcg gaaactatct ggtaaggatc gctataccta agtatatgcg tcatctattt
    24961 ggtgggcggt gtagctacgt taaaagcaca ggcacaagag atattcgaga agcacggttg
    25021 ttccgtgatg cggtggcgct ggaatggata aaactacgta acaagctaaa gcctcaacgt
    25081 gagaaaacat cgaagatcga gcaaattatt acagaactac ggaaagctgg aacatatgca
    25141 tttcaagtat ctgaatcatt taatgattct tctattggct gcccttctct tttaaatatg
    25201 cgggatcagt acattctcca gtttagcgag aagcgcaagc taactacgct atcaaagaca
    25261 aacaaagccg tagaggtgct cttagaacat ctgaataaac gcgatgttca gcttcgtgac
    25321 gttaaccgaa ctattgttac tagctggtta gataacctaa aaaatgagaa agcaccacaa
    25381 acaatacaaa actacattag tgcattagcc cagatttggg atcttgctcg taaccgctac
    25441 cacgatgcac cacaagataa cccttggcga ggtcacggcc ttgaagctaa aagtagcaag
    25501 gtgaattatg aagcctttag caatgatgaa ctcgcaaaag tgttctcaat gctggctgat
    25561 gaagaagaaa tgcaaaatat aacactgatc ggaatgtata caggtatgcg gatcaatgag
    25621 atagcaagtc tcacaataga cgatgtgaaa gagatcgaag gtgtgctgtg ttttgaaatc
    25681 acacagggaa aaacgaaagc tgcggcacgt gttgtgcctg tacatagcct tatcactccg
    25741 ttggtgttgt cgctgcgtga aaagcctcat aatggctttt tgttctatca cgccagcatt
    25801 acagagcgtg ctgatggtaa acgctctacg tggcacacgc agagatttac aagagctaaa
    25861 cgaaaggctt taggggaaaa gggaacagaa aggaaagtgt ttcattctct gagacacgga
    25921 gtagcacagc ttcttgatcg aaatcaaatt ccagaagaca ggatcgccct tctcctgggc
    25981 catacacgcg gcaatacaga gacattccgc acatatagca agaatgcagc ttctccagta
    26041 gagcttaaaa actatattga gcttctacga taccctgaaa tagagaaagg cttatcaatc
    26101 aataaaaaat caaatttaag gcgtaaaaca gcgccataga cgcaataaag aggcttcaac
    26161 atattggtag cctcttttat aattacatcg ttacagagcg ttttagaggc atttacggct
    26221 attcctgttt aaaggcagga gtaatagtgt cttcaccttc actacttgat ttttcagtgt
    26281 taacacgttt tttcatattc gccccgatgt tcttagatgg ctgtgttttg atagctacag
    26341 tgtttacagt gccatacgaa acgagcctga tctgctcaaa gctaagatca aataccaggg
    26401 attcgcttgc ctcttctgtg tattcgatgc ctgttaaaac aacgttctca aaagttttat
    26461 tttctgtaac aagaagaaaa ggctgtctac tgtccataat ctgattcaag tagtctatag
    26521 ctaatgatct tctgttcccg ttcacccctg cactctttaa caagccagga tcgcttttaa
    26581 tcggtgtttc agtgatcctt cctttaagag aaaacttatt gtttttaatt tgaacgtgat
    26641 cagacacagt agcaccattt tctacggggt agcttgtaac atctgccgaa cgtctcacgt
    26701 tcacatcgtc aacactatca aaggccagtg ccatataatt ttcataaaca tcgtttccag
    26761 aagatgccag cccactggta acaatagcaa atccgttctc tccttttgtt ttattagctt
    26821 tgctattttc gttttttgta gagtttatat cttgttctgc tttgtaaaca gcctgaacgt
    26881 gttgttctgg agtatttgct gcacgtatgg ttaaaattcc cgccgttgcc ataataattt
    26941 tccttgtaat gttatggcgg gaattacccg ccgttagatt gtcctgataa gctggttaat
    27001 gttaatgatt tcaagaagct atcccgactt gctctcgctg taaaatccac ataatcgctt
    27061 aatcttgatg tgtccgaatt aatagtaagg ggaacttctg ttaccacttc aaacgttgga
    27121 ttcagattaa acacaggaga taaattataa gccggagttg tcattgcaag attatttaaa
    27181 gcaccctgtt tgagattggt tatcggtgct tcgctgactg acatttttac atctgacaca
    27241 gaagaagact tatcatcgaa aagccatccc ttaatcgtat taaagagaga atgactatta
    27301 tcaggtgcag ttgttgcagt tccagcgcct ttatattggt caggaacgag attagaaaga
    27361 tgctgtttca tcccttcatt gcttaatggc ggtgcatcac ctaaattttt ctgaatagca
    27421 tctgtcttat catcattacg attaaaccag tttacaatct tcataatggc ctcaaagaga
    27481 cttccagacg cttcccctag cttcgtaaaa tacggagtga gattagaaag gtttttcata
    27541 aactcttccg ttacttttgg atcgagagat ttcataaagc cgtctacgaa tgctacttgt
    27601 cttgcatcag aaagctgtga aagctgtctg tttagttctc tgaatttaac gatagattct
    27661 tgctgtgttt ctgttagcca ttttccagag gctttttgct ggttaatact ttcttgtaca
    27721 tctttaacgg aacgttgcca atattttgat gtaagcatca aatcatcgcc caggtcttct
    27781 aaacgttgtc caatttggga gtctgaaaac ccttttttca tcattccatt aacagcctta
    27841 gaaattaatc cagccggatt atcagcaaat tttttaaggt cgcttttgtt aataacaccg
    27901 atgtttagaa gttcattaat agcgttatca ccgcctttcc atttagattt tccggtttgc
    27961 ttatctttca ccaattcagc ttcactgtag gattttgcgg cacgttccct aacatctttc
    28021 atctgatcca ggtatttcct agccccttga tcaccctgga ggctatcgat cccgtgtcta
    28081 taggcccatt ctgtaatgtt atacatctgg ttaacgtcta caccgcccag cttggcccgt
    28141 tgaattagct ccccttgcgt ctctgcgtta gcgaagctgc tagtaacaaa atcagaggct
    28201 ttgcttacag ccatcacgcc agcagcagcc gtagcgcctc caaataacag agcgcccata
    28261 gtggcggaat tccctgatcc tcctccctta acgggggcta attgagcttt tctgttgcca
    28321 ttaatcttac gttgttgctg ttgtaggcgt ttcatttggc tattcatacg tgcaagcgag
    28381 atagcccctt gctcatattg ttttgctatc tctctagcct gtgcaatagc tttgtattgt
    28441 tccgctacag agagacggtg cattgcactg atagagcttg acacatcgag gagctttaat
    28501 tctgctttct ccttacgtgc tgctatttgt tgctgttgcc ttgcctgtgc tcttgctgcg
    28561 gcagcttgtg ctctttgatt ctgtttttcg tttttcgctt tctgttgagc aatcttatct
    28621 acttcgcttt gtgctttctt atagccttta gtgacattat caaatcttaa tttagctggc
    28681 ttgatgtttt ctgcgaattc ctttaagcct gtaatagagt ctttcgcttt tttcaagctg
    28741 tctttatcta cactaaatgt tactgtgttc ttaattttag aagtgttaat ttctaacatt
    28801 taaaatcccc cttattaatc atttaccacc ttctttactt aagaaatggt gtagagcctg
    28861 atcaatactc tcgtttagtc tgaacatacg ggcttttact ccttcatagg gaacatctaa
    28921 aagagaacat agatatttca gatttaacaa gcctctagaa tcattagcat ctaaatgtac
    28981 gtgtacacca ttttttgatt tcagaatcac ttccatattt taatcacctc tcaaaattta
    29041 aaaaataaat aaaaaaaagg cgaagcgtga atacacgcca cgcctctatt acacgttaaa
    29101 atgttattta acagtgattt gaacagagaa gccaacgccg tggtttacaa ccatctggct
    29161 attctctgta acaacgttaa cagcatcacc acgagaagat tcatagctgt agctgtacac
    29221 ctcagcagga gccgcattga tcagttcaaa ggtgctggaa gccgccccga agatgttggc
    29281 ataaacacct gcccccttag caaacttcgg aaccgctaca gcgtctgcgg tcatatgtgc
    29341 aaggtgtagc ggatctacca gtttaataac atcaatgtta gttcctggaa tagtgaacgc
    29401 tgtcaccccc ggtagcaggt cacgacgctg gtacacaaca tttccctctt ccagtgggct
    29461 tacatactgg aatgcgtttg ccatacccgc agagaatcga attgcagaga aggcttccgg
    29521 cttacagaaa acaacaatac gctctacagc agccgcctgg ctttgtgctt tctcgataat
    29581 gtcagtagca atctgatcaa attctttaaa aatcttcgtg gtgtcagtgc tggaagcgtt
    29641 cacagtggct ttcatagctg atacgttaaa cagcttgccc cactcgataa gaacatcatc
    29701 agttttcggg gtgtaggtgc gaccactaaa cagagaagca gccaatacgc tctcttttgt
    29761 acgctgatac gctacgtggt gtttagcaac atattccgcg tagatatcag tgagtgtttc
    29821 ctggaaatca gtgccaggtt tacgtttgcc ttgaacatca gaaggacgga tcacatcttc
    29881 acgcaggaag taagggatct cgatcagcca ttctttacct tgttctcgct tagtaacgtt
    29941 atgctcagaa gagaaacgag aggtttcttt gttgaacaga gattcgttat tttcgacaag
    30001 ctgggaaaca gctactttat gagaactaac gcccaccgga tcagtaaaat ccagagtaga
    30061 aagcagataa tttctagtgc tatgcgcaga gaacagagga gctaaatcaa cgtaatcatt
    30121 acctagaatc atttattatt attcctttta ttaagaagtg aaaacagaat ggaacccttc
    30181 aagctggaag ccttttttaa ttaatgctgt ttttgctgct tcacttacag cattcagcgc
    30241 atcagctttg atgcagacaa gcccagcatt gctacgtaaa acatctacag gcttgttttc
    30301 tccagccgtt acaaattcac tcataacaac atagatatca tctcctgatt catatttagc
    30361 gcccgtgctg gtgatcaagt cgccgcattc aacatcagca ggaagagaag caaaaacctt
    30421 gttttctaca gaggcaaacg gatcagtaca ataacttaca atatctgtga aagtctttcg
    30481 aactggaaac agagtgtcta aatcaataat catttagtaa tctccttagc ttttaatttg
    30541 attaattcat taagggaagg tgtagacgga gaagaaggag aaaaatattt ttcaacacat
    30601 ctttccactt cctctacaga acacccagcg tatttagcta ccatttcggt aatttg
//
LOCUS       DAZGVQ010000047        30215 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000047 DAZGVQ010000000
VERSION     DAZGVQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.047"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            204..1148
                     /gene="ydgH"
                     /locus_tag="ACHLAG_002036"
     CDS             204..1148
                     /gene="ydgH"
                     /locus_tag="ACHLAG_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFP0655275.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            1334..2716
                     /gene="ydgI"
                     /locus_tag="ACHLAG_002037"
     CDS             1334..2716
                     /gene="ydgI"
                     /locus_tag="ACHLAG_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0655276.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            2753..3475
                     /gene="folM"
                     /locus_tag="ACHLAG_002038"
     CDS             2753..3475
                     /gene="folM"
                     /locus_tag="ACHLAG_002038"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFP0655277.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(3472..3807)
                     /gene="ydgC"
                     /locus_tag="ACHLAG_002039"
     CDS             complement(3472..3807)
                     /gene="ydgC"
                     /locus_tag="ACHLAG_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFP0655278.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            3936..4655
                     /gene="rstA"
                     /locus_tag="ACHLAG_002040"
     CDS             3936..4655
                     /gene="rstA"
                     /locus_tag="ACHLAG_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFP0655279.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            4659..5960
                     /gene="rstB"
                     /locus_tag="ACHLAG_002041"
     CDS             4659..5960
                     /gene="rstB"
                     /locus_tag="ACHLAG_002041"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFP0655280.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            6036..6965
                     /gene="tus"
                     /locus_tag="ACHLAG_002042"
     CDS             6036..6965
                     /gene="tus"
                     /locus_tag="ACHLAG_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFP0655281.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(6962..8365)
                     /gene="fumC"
                     /locus_tag="ACHLAG_002043"
     CDS             complement(6962..8365)
                     /gene="fumC"
                     /locus_tag="ACHLAG_002043"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFP0655282.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(8508..10154)
                     /gene="fumB"
                     /locus_tag="ACHLAG_002044"
     CDS             complement(8508..10154)
                     /gene="fumB"
                     /locus_tag="ACHLAG_002044"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP0655283.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAL
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10353..11528
                     /gene="manA"
                     /locus_tag="ACHLAG_002045"
     CDS             10353..11528
                     /gene="manA"
                     /locus_tag="ACHLAG_002045"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFP0655284.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            11629..13137
                     /gene="ydgA"
                     /locus_tag="ACHLAG_002046"
     CDS             11629..13137
                     /gene="ydgA"
                     /locus_tag="ACHLAG_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFP0655285.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(13182..14447)
                     /gene="uidC"
                     /locus_tag="ACHLAG_002047"
     CDS             complement(13182..14447)
                     /gene="uidC"
                     /locus_tag="ACHLAG_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFP0655286.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLVDDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(14486..15859)
                     /gene="uidB"
                     /locus_tag="ACHLAG_002048"
     CDS             complement(14486..15859)
                     /gene="uidB"
                     /locus_tag="ACHLAG_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFP0655287.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(15856..17667)
                     /gene="uidA"
                     /locus_tag="ACHLAG_002049"
     CDS             complement(15856..17667)
                     /gene="uidA"
                     /locus_tag="ACHLAG_002049"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFP0655288.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRIFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(18056..18646)
                     /gene="uidR"
                     /locus_tag="ACHLAG_002050"
     CDS             complement(18056..18646)
                     /gene="uidR"
                     /locus_tag="ACHLAG_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFP0655289.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(18875..19642)
                     /gene="hdhA"
                     /locus_tag="ACHLAG_002051"
     CDS             complement(18875..19642)
                     /gene="hdhA"
                     /locus_tag="ACHLAG_002051"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFP0655290.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(19754..20782)
                     /gene="malI"
                     /locus_tag="ACHLAG_002052"
     CDS             complement(19754..20782)
                     /gene="malI"
                     /locus_tag="ACHLAG_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFP0655291.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            20957..22549
                     /gene="malX"
                     /locus_tag="ACHLAG_002053"
     CDS             20957..22549
                     /gene="malX"
                     /locus_tag="ACHLAG_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFP0655292.1"
                     /translation="MTAKTAAKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            22559..23731
                     /gene="malY"
                     /locus_tag="ACHLAG_002054"
     CDS             22559..23731
                     /gene="malY"
                     /locus_tag="ACHLAG_002054"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFP0655293.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            23835..24836
                     /gene="add"
                     /locus_tag="ACHLAG_002055"
     CDS             23835..24836
                     /gene="add"
                     /locus_tag="ACHLAG_002055"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFP0655294.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(24871..25911)
                     /gene="ydgJ"
                     /locus_tag="ACHLAG_002056"
     CDS             complement(24871..25911)
                     /gene="ydgJ"
                     /locus_tag="ACHLAG_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP0655295.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            26154..26279
                     /gene="blr"
                     /locus_tag="ACHLAG_002057"
     CDS             26154..26279
                     /gene="blr"
                     /locus_tag="ACHLAG_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFP0655296.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            26552..26767
                     /gene="ydgT"
                     /locus_tag="ACHLAG_002058"
     CDS             26552..26767
                     /gene="ydgT"
                     /locus_tag="ACHLAG_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFP0655297.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            26853..27293
                     /gene="ydgK"
                     /locus_tag="ACHLAG_002059"
     CDS             26853..27293
                     /gene="ydgK"
                     /locus_tag="ACHLAG_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFP0655298.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            27370..27951
                     /gene="rsxA"
                     /locus_tag="ACHLAG_002060"
     CDS             27370..27951
                     /gene="rsxA"
                     /locus_tag="ACHLAG_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFP0655299.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            27951..28529
                     /gene="rsxB"
                     /locus_tag="ACHLAG_002061"
     CDS             27951..28529
                     /gene="rsxB"
                     /locus_tag="ACHLAG_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFP0655300.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            28522..>30215
                     /gene="rsxC"
                     /locus_tag="ACHLAG_002062"
     CDS             28522..>30215
                     /gene="rsxC"
                     /locus_tag="ACHLAG_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFP0655301.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIAR"
BASE COUNT         7319 a         7770 c         7442 g         7684 t
ORIGIN      
        1 aattcagttg cttcagtagt aatgatgctg atacggctgt tttttaagca tagacggtca
       61 tttgagcagg attaaaattg gcttaaggaa tgtgatatga aaaatgacgc agacagttac
      121 accgtttaaa tgcaataatc agccacgttt ctcgttaata acaataccag tacctggttt
      181 gcgcaaggcg aaggattatt tttatgaagc ttaagaacac cctcctggcg tcggcactgc
      241 tttctgctac ggcattctcc gttaacgcag caacagaact gacaccggag caagcggcag
      301 cggttaaacc ttttgaccgt gtagtggtta ccggtcgttt taatgctatt ggcgaagcgg
      361 tgaaagccgt ttctcgtcgc gcagataaag aaggtgccgc ctctttttat gttgtcgaca
      421 cttctgattt tggtaacagc ggtaactggc gtgtggtcgc tgacctctat aaagccgatg
      481 ctgaaaaagc agaagaaaca agtaatcgcg taattaacgg tgttgtcgaa ctgccgaaag
      541 atcaggctgt tctgattgaa ccgtttgaca cggtcaccgt ccagggcttc tatcgtagcc
      601 agccagaagt caatgatgcc atcaccaaag cagcaaaagc gaaaggtgcc tactctttct
      661 acattgttcg tcaaatcgat gccaaccagg gcggcaacca gcgtattact gcattcatct
      721 ataaaaaaga tgctaagaaa cgtatcgtcc agagcccgga tgtgatcccg gcagattccg
      781 aagcaggacg tgcagctctg gctgccggtg gcgaagccgc gaagaaagtt gagatcccag
      841 gtgttgcgac taccgcatca ccaagttctg aagtcggtcg tttctttgaa acccagtcat
      901 caaaaggcgg acgttacact gtcacgcttc ctgacggcac taaagtcgaa gaactgaaca
      961 aagcgacagc agcgatgatg gtcccgttcg acagcatcaa attctctggc aactacggca
     1021 acatgaccga agtctcctat caggttgcga aacgtgccgc gaagaaaggt gctaagtatt
     1081 accacatcac ccgccagtgg caggaacgtg gtaataacct gaccgtcagc gcagatctgt
     1141 ataaataact attcagttac tcctataagg cggcttgatg ccgcctttcg catttttagt
     1201 cacaaatttc cccgcgacca ttgcatgcag acacatctct tcgtaaaatc ccgcgcttca
     1261 gtggcaaact ccatttttat gcgttttgcc ctaatattta ttcttcttat cacgttttaa
     1321 tcactggata tcgatggaaa agaaactggg actgagcgca ctcaccgcgc tggtattaag
     1381 ctcaatgctg ggcgcgggtg ttttcagtct gccgcaaaat atggcggcag ttgccagccc
     1441 ggcagcactg ctcatcggct ggggtattac tggcgctggc attttattgc tggcctttgc
     1501 catgctgatc ctcacgcgca ttcgtcccga acttgacggc ggtatcttta cctatgcccg
     1561 cgaagggttt ggcgagctaa tcggcttttg ttccgcatgg ggatactggc tgtgcgcagt
     1621 catcgccaac gtctcctatc tggtgatcgt tttttccgcg ttaagctttt ttacggacac
     1681 gccggaattg cgcctgttcg gcgatggtaa tacctggcaa tcgatagtcg gtgcatcggc
     1741 tttattgtgg attgttcact ttttgatcct gcgcggggtg caaaccgctg ccagcattaa
     1801 cctggtggcg acactggcaa aattgttgcc gctgggtctg tttgttgtgc tggcgatgat
     1861 gatgttcaaa ctggatactt tcaagctcga cttcaccgga cttgcacttg gcgtacccgt
     1921 gtgggaacag gtgaaaaaca ccatgctgat caccctgtgg gttttcattg gtgtggaagg
     1981 tgccgtggtt gtttctgcgc gtgcgcgtaa taaacgtgat gttggcaaag cgacactgct
     2041 ggcggttctc tccgctctgg gcgtttactt gctggtaacg ctgctttcgc tgggcgtggt
     2101 ggcacgtcct gaactggctg aaattcgtaa cccgtcaatg gccggtctga tggtggaaat
     2161 gatgggacca tggggcgaaa tcatcatcgc tgccggtttg atcgtttccg tttgcggcgc
     2221 gtacttaagc tggaccatca tggcggcaga agttccgttc ctggctgcca ctcataaagc
     2281 attcccacgc atttttgcgc gccagaacgc acaagctgcg ccatccgcct cgctgtggtt
     2341 gaccaacatc tgcgtgcaaa tttgtctggt actcatctgg ctgaccggtt ccgattacaa
     2401 cacgttgctg accatcgcct cagaaatgat tctggtgccc tatttcctgg tcggcgcgtt
     2461 cttgctgaaa atcgcaaccc gtccactgca taaagcggta ggtgtcggtg cctgcattta
     2521 tggcttatgg ttattgtatg cctctggtcc aatgcacttg ctgttatccg ttgttctgta
     2581 tgcgccgggg ctgctggttt tcctgtatgc gcgcaaaacg catacacatg ataatgtact
     2641 gaaccgtcag gaaatggtgc tgattggcat gctgttaatt gcctcagttc cggcgacctg
     2701 gatgctggtg ggataacgtt catcccatcg ttggatagca aaggagataa cgatgggtaa
     2761 agcccagccc ttgccaatat taattactgg cggaggtcgt cgcatcggcc tcgccctcgc
     2821 ctggcatttc attaatcaaa agcaaccggt gattgtcagc tatcggacgc actatccagc
     2881 cattgatgga ctgattaagg caggtgcgca gtgtattcag gctgattttt cgaccaacga
     2941 cggtgtgatg gcgtttgccg atgaagtact aaaaagcacc catggtctgc gtgctatttt
     3001 gcataacgcc agtgcgtgga tggcggaaaa accaggtaca ccactggccg acgtactggc
     3061 ttgcatgatg cagatccacg ttaatacccc atacctgctc aaccatgcgc tggaaagatt
     3121 actgcgtggg cacggacacg ccgccagcga tatcattcac tttaccgatt atgtggtgga
     3181 gcgcggtagc gacaaacatg ttgcgtatgc tgcaagcaaa gcggcactgg ataatatgac
     3241 ccgctcgttt gcccgcaagc tggcaccgga agtgaaagtg aattctattg cgccatcgct
     3301 gatcctgttt aatgaacatg atgatgccga atatcgacaa caggcgctga ataaatcact
     3361 gatgaaaacc gcgcctggcg agaaagaagt gatcgacctg gtcgattact tacttaccag
     3421 ctgctttgtc accggacgca gtttcccact tgatggcggt cgtcatctgc gttaatgcag
     3481 ctttatccag catataatca acacccatgc gcttatcccc caacacgcaa cagaaccaac
     3541 aaaagcggcg ggcaagcgca tcattccggt gaaataccac agcgacacca ggtaaacaaa
     3601 atagggaata atcgaccaca tactaaaaat gatggttgcg cgtaaggctt caatgccgcg
     3661 ttcgctggca acaatataat gcgcgataag cgcaaaggtc gggaaaagtg gaatcagccc
     3721 ggcgatataa taatttttcg tttttgctaa aacaccaatc aacagcacta ccagcgcacc
     3781 gagcgcggct ttgattacca gccccatctt tttaccttaa cacttccata acaagtcatc
     3841 agtagaatac ctgatgaaaa cttgtttaga aacgattgat agtaagtaaa aacagcgcgg
     3901 tgtattgtga cgtttttata tctaccgtga atgttatgaa cactatcgta tttgtggaag
     3961 atgatgcgga agtcggttca ctgattgccg cgtacctggc aaaacatgat atgcaggtta
     4021 ccgtagaacc gcgcggcgac caggccgaag aaaccatttt gcgagaaagt ccggatttgg
     4081 tgttactcga catcatgcta ccaggcaagg atggcatgac catttgtcgt gatttacgcg
     4141 caaagtggtc tggaccgatt gttcttctaa cctctctcga tagcgatatg aaccacatcc
     4201 tggcactgga aatgggtgcc tgcgactata ttctcaaaac gacgccccct gctgttttgc
     4261 tagcgcgttt acgtttgcat ttgcgtcaga atgagcaagc cacactgacc aaaggtcttc
     4321 aggaaacgtc tctgactccc tacaaagccc tgcatttcgg cacgttgacc atcgatccca
     4381 tcaaccgcgt agtcaccctg gctaacactg aaatctcgct ctcgacagct gatttcgaat
     4441 tattgtggga attagctacc catgccgggc aaatcatgga ccgcgatgca ttgctgaaaa
     4501 atttacgcgg cgtcagttat gacggactgg atcgtagcgt ggacgtggct atttcgcggt
     4561 taagaaaaaa actgctcgat aacgccgcag aaccttatcg cattaaaact gtgcgtaaca
     4621 aaggctatct ttttgcgcct catgcatggg aataagcgat gaaaaaactg tttatccagt
     4681 tttacctgtt attgtttgtc tgcttccttg tgatgtctct gctggttggg ttggtgtaca
     4741 aatttaccgc cgaacgcgcg ggcaaacagt cgctggatga tttgatgaac agttcgctgt
     4801 atctgatgcg cagcgaattg cgtgagatcc ccccacacga ctggggtaag acgctgaaag
     4861 agatggattt aaatctctct ttcgatctgc gtgtcgagcc attgagtaaa taccatcttg
     4921 atgatatttc catgcaccga ctgcgtggcg gcgaaattgt cgccctggac gatcagtaca
     4981 cgtttttgca gcgtatcccg cgcagccact acgtgctggc agttggtcct gttccttatc
     5041 tttattatct ccatcagatg cgattgctgg atatcgccct gatcgctttt attgctattt
     5101 ccctcgcctt tccggtgttt atctggatgc gtccgcactg gcaggatatg ttaaaactgg
     5161 aagcagcggc gcaacgattt ggcgatgggc atctcaatga gcgtatccac tttgatgagg
     5221 gttcgagctt tgaacgactt ggtatcgcat ttaaccagat ggcggacaat atcaacgcct
     5281 tgattgccag caaaaaacag cttattgacg gtatcgctca cgaactgcga acaccgttag
     5341 tgcgcctgcg ttatcgactg gagatgagcg ataacctgag cgccgccgaa tcccaggcgt
     5401 tgaatcgtga tatcagtcaa cttgaagctt taattgaaga gctgctgact tatgcccgac
     5461 tcgatcgccc acaaaacgag cttcatctta gcgaaccaga cctgccgttg tggctgtcaa
     5521 cgcatctggc agacattcag gcagtaacgc ccgataaaac ggtacggatt aaaacgctcg
     5581 tgcaaggcca ttatgcggcg ttggatatgc gcttaatgga gcgcgtgctg gataatttgc
     5641 tcaataacgc cctgcggtac tgccattcaa cggttgaaac cagcctgcta ctgtcgggga
     5701 atagagcgac attaattgtt gaggatgatg gcccagggat tgccccagaa aaccgcgaac
     5761 atatctttga accttttgtt cgcctcgatc ccagccggga tcgctcaacc ggcggctgcg
     5821 ggctggggct ggcaattgtc cactctatag cactggcaat gggcggtacg gttaattgtg
     5881 acaccagcga actgggtggt gctcgcttct cgtttagctg gccgttatgg cataacatcc
     5941 cgcaatttac ctctgcctga cactacgcgc acgatggtca agtcaccacg actgtgctat
     6001 aaaataagta tgttgtaact aaagtggtta atattatggc gcgttacgat ctcgtagacc
     6061 gactcaacac tacctttcgc cagatggaac aagagctggc tacatttgcc gctcatcttg
     6121 agcaacacaa gctattggtt gcccgcgtgt tctctttgcc ggaggtaaaa aaagaggatg
     6181 agcataatcc gcttaatcgt attgaggtaa aacaacatct cggcaacgac gcgcagtcgc
     6241 tggcgttgcg tcatttccgc catttattta ttcaacaaca gtccgaaaat cgcagcagca
     6301 aggccgctgt ccgtctgcct ggcgtgttgt gttaccaggt cgataacctt tcgcaagcag
     6361 cgttggtcag tcatattcag cacatcaata aactcaagac cacgttcgag catatcgtca
     6421 cggttgaatc agaactcccc accgcggcac gttttgaatg ggtgcatcgt catttgccgg
     6481 ggctgatcac ccttaatgct taccgcacgc tcaccgttct gcacgacccc gccactttac
     6541 gctttggttg ggctaataaa catatcatta agaatttgca tcgtgatgaa gtcctggcac
     6601 agctggaaaa aagcctgaaa tcaccacgca gtgtcgcacc gtggacgcgc gaggagtggc
     6661 aaagaaaact ggagcgagag tatcaggata tcgctgccct gccacagaac gcgaagttaa
     6721 aaatcaaacg tccggtgaag gtgcagccga ttgcccgcgt ctggtacaaa ggagatcaaa
     6781 aacaagtgca acacgcctgc cctacaccgc tgattgcact gattaatcgg gataatggtg
     6841 cgggcgtgcc agacgttggt gagttgttaa attacgatgc cgacaatgtg cagcaccgtt
     6901 ataaaccaca ggcgcagccg cttcgtttga tcattccacg gctgcacctg tatgttgcag
     6961 attaacgccc ggctttcata ctgccgacca tctgttctgg ccgtacccag ctgtcaaact
     7021 cggcttcgct aagatacccc agcgcaaggg ccgcagcttt taaggtcagc ccttctttat
     7081 gcgctttttt ggcgatctcc gcggctttgt cataaccaat gtgggtgtta agcgcagtca
     7141 ccagcatcag cgattcattg agtaattgat tgattcgctc acgattcggt tcaataccca
     7201 ctgcgcagtg tttgttaaaa ctttccatgc catctgccag caagcgcacc gattgcagga
     7261 aattatggat caccatcgga cggaagacgt tcagttcaaa gttaccggaa gcgccaccca
     7321 tgttgatcgc cacgtcgttc cccattacct gacagcagag catggttaat gcttcacact
     7381 gggttgggtt cactttcccc ggcatgattg agctgcccgg ctcattttcc gggattgaga
     7441 tttcaccaat tccgcagcgc gggccagagg ccagccagcg gacatcattg gcgattttca
     7501 tcagtgacgc agccaaccct ttcaacgcgc cgtgcgcctg aaccagggca tcacaggtcg
     7561 caagcgcttc aaatttgttc ggcgcggtaa caaacggtgc acaggtaatg actgccagtt
     7621 catctgctac gcgacgcgca tactccggat gggtatttag tccagtaccc accgctgtac
     7681 cgccaagagc cagttccgct acgtgaggca ggctgtattc gatatgtttg agattatgct
     7741 cgagcatcgc tacccagccg gaaatctcct gccctagtgt tagcggcgtg gcgtcctgca
     7801 agtgggttcg accgattttg acgatatcgg caaatgcacg cgatttttca ctcagtgtct
     7861 gtgtcagggt tttaagctgc ggaatgagtt gcttgcgcag cgccagcagc gccgcaacgt
     7921 gcatcgccgt cggaaagaca tcgttggaac tttggctttt gttcacgtcg tcgttagggt
     7981 gaactttacg ttccatcccg cgcacgccgc cgagtaattc actggcccgg ttagccagca
     8041 cttcgttcat gttcatgtta ctttgcgtgc cggagccggt ctgccagata gccagcggga
     8101 attcgtcgtc atgctgtcct gccagtactt catccgccgc ctgccgaatg gcgctcgctt
     8161 tctcttcaga caacaagcct aaatcttcat taacttttgc cgctgcgcgc ttggttagcg
     8221 ccagcgcatg aatcagtgag gtgggcattt tctccgtcga aatgcggaaa tgctccagcg
     8281 agcgttgagt ttgtgcgccc cacagcttat ctgccgggac atcaatcgcc cccatcgaat
     8341 ctttttcgct gcgtactgta ttcatggcct gctcctcacc tgtttaattt tttctttctg
     8401 ttttgctttc gttaagcaac ttttagctca cttattattt actatttgat aacaaatgtt
     8461 tggtctttcg tgccatgtaa aaaaaccgcc ccgaagggcg gctctgttta tttcacacag
     8521 cgagtgcact gtgtgagttg tatctgctgg aagaagtcat ttcctttatc atccacaagg
     8581 ataaacgccg ggaaatcttc cacttcaatt ttccagatgg cttccattcc cagttccgga
     8641 tattcaacac attccaggct cttaatactt ccctgcgcca atacagcggc cggaccaccg
     8701 atactgccaa ggtagaagcc gccgtgtttt ttacaggcat ccgtcacctg ctggctgcgg
     8761 ttgcctttcg ccagcatgat catacttccg ccctgcgctt gcagttgatc gacataagaa
     8821 tccatccgtc cggcggtcgt tgggccaaga gaaccggagg cataaccttc cggcgttttg
     8881 gccggacccg cgtagtaaat cggatgatct ttaatgtact gcggcagccc ttcaccgtta
     8941 tccatccgct ctttcagttt ggcgtgagca atatcacgac cgacgataat cgtgccgtta
     9001 agcgataagc gtgtagaaac gggatactgc gacaactgtg cgaggatctc tttcatcgga
     9061 cggttaaggt caacgcgcac cgcttcgcct tctcccgctt tacgcagctc ttcagggata
     9121 tatttacctg gattatgttc cagtttttcg atccagatcc cctgacggtt gatcttcgct
     9181 ttgatattac ggtcagcaga gcaggaaaca cccataccaa ccggacagga tgcgccgtga
     9241 cgtggcaggc gaatcacgcg tatgtcgtga gcgaagtatt taccaccaaa ctgcgcaccc
     9301 agaccgagat tttgcgcttc gatcagcaat tctttttcca gttccacatc gcggaatgcc
     9361 tgaccgtgct cattcccttc cgttggcagt tcgtcatagt atttagcgga agccagtttc
     9421 actgttttaa ggttagtttc tgcagaagtt ccaccaatta cgaacgcaat atgatacgga
     9481 ggacaggccg ccgtacccag cgtgcgcatc ttctcaacca ggtaattttt cagtttcccc
     9541 ggcgttagta acgctttggt ttcctgatag agatacgtct tgtttgccga accaccacct
     9601 ttggcgatac agaggaattt gtactcgtcg ccatcaacgg cataaagatc gatctgcgct
     9661 ggcagattgg tgccggtatt cacctcttta tacatatcca gcggcgcgtt ttgcgagtag
     9721 cgcaaattat cttcgatata agtgttatag acaccgcgcg ccagcgccgc ttcatcacag
     9781 ccaccggtcc atacacgctg ccctttttta ccaacaataa tcgcagtgcc ggtatcctga
     9841 caggttggca gaacgccttt cgccgcgatg tcggagttac gcaggaattg cagcgccaca
     9901 tatttatcat tttcgctggc ctccgggtca cgcagaatgt cggccacctg ttgttggtga
     9961 gccggacgca gcataaacga cgcatcatga aacgcctgac gcgccaacag agttaacgct
    10021 tcgagtgcga ctttcaaaat ctcctgccct tcaaattcag atacgctaac gtgttcgctg
    10081 gttagcaggt aatactcagt atcatctttt ttgagtggaa aaggagcctg ataatgaaag
    10141 ggtttgtttg acattgttct ctcacttact gcctggtttg gttatgctct gggcgggtgt
    10201 tccgttgccc tgttaaaagc gagtaacaat atcctacaca cttttttaac aaaaactgag
    10261 actagtacga ctttttgcgg ctccaggtta cttcccgtag gattcttgct ttaatagtgg
    10321 gattaatttc cacattaaaa cagggattga tcatgcaaaa actcattaac tcagtgcaaa
    10381 actatgcctg gggcagcaaa acggcgttga ctgaacttta tggtatggaa aatccgtcca
    10441 gccagccgat ggccgagctg tggatgggcg cacatccgaa aagcagttca cgagtgcaga
    10501 atgccgccgg agatatcgtt tcactgcgtg atgtgattga gagtgataaa tcgactctgc
    10561 tcggagaggc cgttgccaaa cgctttggcg aactgccttt cctgttcaaa gtattatgcg
    10621 cagcacagcc gctctccatt caggttcatc caaacaaacg caattctgaa atcggttttg
    10681 ccaaagaaaa tgccgcaggt atcccgatgg atgccgccga gcgtaactat aaagatccta
    10741 accacaagcc ggagctggtt tttgcgctga cgcctttcct tgcgatgaac gcgtttcgtg
    10801 agttttccga gattgtctcc ctactccagc cagtcgcagg tgcacatccg gcaattgctc
    10861 actttttaca acagcctaat gccgaacgtt taagcgaact gttcgccagc ctgttgaata
    10921 tgcagggtga agaaaaatcc cgcgcgctgg cgattttaaa atcggcgcta gacagtcagc
    10981 agggtgaacc gtggcaaacg attcgtttaa tttctgaatt ttacccggaa gacagcggcc
    11041 tgttctcccc gctgttactg aatgtggtga aattgaatcc tggcgaagcg atgttcctgt
    11101 tcgctgaaac accgcacgct tacctgcaag gcgtggcgct ggaagtgatg gcgaactccg
    11161 ataacgtgct gcgtgcgggt ctgacgccta agtacattga tattccggaa ctggttgcca
    11221 atgtgaaatt cgaagccaaa ccggctaacc agttgttgac ccagccggtg aaacaaggtg
    11281 cagaactgga cttcccgatt ccagtggatg attttgcctt ctcgctgcat gaccttagtg
    11341 ataaagaaac caccattagc cagcagagtg ccgccatttt gttctgcgtc gaaggcgatg
    11401 caacgttgtg gaaaggttct cagcagttac agcttaaacc gggtgaatca gcgtttattg
    11461 ccgccaacga atcaccggtg actgtcaaag gccacggccg tttagcgcgt gtttacaaca
    11521 agctgtaaga gcttactgaa aaaattaaca tctcttgcta agcttattaa aggcttataa
    11581 caccttcagg cggccagtcc gcctgatttc attttatgga taatcattat gaataaatcg
    11641 ctggtagcgg taggcgtcat tgttgcgcta ggcgtagtct ggacaggcgg cgcatggtat
    11701 acaggcaaga agattgaaac ccatctcgaa gacatggtcg cgcaggcgaa cgcgcaactc
    11761 aaactgacag ctcctgaatc caacctggaa gtgagttatc aaaactatca tcgcggcgta
    11821 ttcagcagcc agttgcaact gttggtgaaa cccattgccg ggaaagaaaa tccgtggatt
    11881 aaaagcggtc agagcgtcat cttcaacgaa tcggttgatc atggtccctt cccgcttgcc
    11941 cagcttaaaa aactgaacct gatcccgtcg atggcatcaa ttcaaaccac gctggttaat
    12001 aacgaagtaa gcaaaccact gtttgatatg gcaaaaggtg aaacgccttt tgagattaac
    12061 tcgcgcattg gttacagcgg tgattccagt tccgatattt cgctcaagcc actgaattac
    12121 gagcaaaagg atgaaaaagt cgcctttagc ggcggcgagt tccagttaaa tgctgacaga
    12181 gacggcaaag ccatctccct ttccggggag gcgcaaagtg gacggataga cgcagttaac
    12241 gaatataacc agaaagtgca gttgaccttt aataatctga aaaccgacgg ttccagcacg
    12301 ctggcaagtt ttggtgagcg cgtaggaaac caaaaactgt cactggaaaa aatgaccatt
    12361 tcagtggaag gcaaagaact ggcactgctg gaaggcatgg agatcagcgg taaatcggat
    12421 ctggtcaatg acggtaaaac gatcaatagc caactggatt actcgctaaa cagcctgaag
    12481 gtacagaatc aggatctggg cagcggcaag ctgactttaa aagtcggcca gattgatggt
    12541 gaagcctggc atcagtttag ccagcaatat aacgcgcaaa ctcaggcgct gctggcacag
    12601 ccagaaattg ccaacaatcc cgaactttat caggagaaag tgacggaagc cttctttagc
    12661 gccctgccgc tgatgttgaa aggcgatccg gtgattacta tcgcgccgct aagctggaaa
    12721 aacagtcagg gtgaaagtgc gctgaatctg tcgctgttcc tgaaagatcc ggcaacgact
    12781 aaagaagcgc cgcaaacgct ggcgcaggaa gtagatcgtt cggttaaatc tctggatgcg
    12841 aaactgacca ttccggtgga tatggcaact gagtttatga ctcaggtagc gaagctggaa
    12901 ggttatcagg aagatcaagc gaaaaaactg gcgaaacagc aagttgaagg tgcatcagca
    12961 atggggcaga tgttccgtct gaccaccttg caggacaata ccatcaccac cagcctgcaa
    13021 tatactaacg gtcagataac gttaaacggg cagaaaatgc cactggaaga tttcgttggt
    13081 atgtttgcaa tgccggcatt aaatgttccg gtcgtacccg ctattccgca gcagtaattc
    13141 acagacaaaa cgccggagat ttttctctcc ggcgttattt tttacttcag cataaagtca
    13201 tactgaatac tcattttcac atcccagtaa ttcaggcgat cgcccagcgt actgccatcg
    13261 cgcttttccc gccctttatc ggcagcaaga aagaaattca tcccttgtaa cggcccttcg
    13321 ctgacccgat agttcatacc acctaagata cccgtgcctt caatccgcac atctcgttta
    13381 ctggattcat agccgcgagt cacaatcggt gcaaatgtca gcgtaaattg cggcgttaaa
    13441 cgatagttga cgcccaattg ccaggactgc attccttcac ggttacgatc aagacttaat
    13501 gaaaagggat agccaatgtc ggtatccacc acggcaccat tcattgcatg gcgcaaataa
    13561 ccaccactgc caaatacgct tccccacggc gcattccagg tcaattgtcc actaaccaac
    13621 gccgtttcat tgggctggct ggtattacga cttaaacctt cgagctgcgc gtaaaagccg
    13681 agcaactccc ctttcaacgt ataattcggg tcgagcggta tgcttttctc gagaatactt
    13741 tgtatctgat tggaaacatc tttctgtacc gatgccccca gagaatagcg actggtatca
    13801 tcatgccaac tggcggctaa aaaagaacgt gcgcgatgtt tatcgtattt tcccgtctgg
    13861 ctgttataca ccccttcatc acgaataccg gttgttgaat cagtccataa aaatactcgt
    13921 gaacgccaca ttccccacag atcaatattt ggtgttaaag cccaataacc taatgctcct
    13981 tcagaaactg tcggtaatgt acgttgcgaa ctgccagcca ttagcggaat acgataaggt
    14041 aagctaccat aactataatc cgtcccgaag cgacctaatt taagcaggct attttcactg
    14101 ggttttattt ttactgcccc cagggcaaag ccaaaacttt tatcaccatc aagataccac
    14161 cgggtactca tatcagcacg agcacctaat ttataaacat aatatgcccc gccttcaacg
    14221 ccaataatat tggaataata accactattg aaatcgagca atcctccctg tacccatgcg
    14281 taaatatctc ttcccgcgcc agcactgggc ttatccgccc tgcgcaattc gtttttcagt
    14341 ctgatgcgta gtcccgcttc atcgtcaacc agttgcgccg cataagcaga ggtaaggcca
    14401 gaggcagccg tcaggcaaat aacggccatg gccactattt ttctcatagt tgcacctttg
    14461 atgttctgat tatttttatt gaatattaat tagtgatatc gctgattaat tgctgctgca
    14521 cttttttacg attatcaatt tcaaccacga tttctttgaa ttttttatcc gtgagcggat
    14581 aaaaccagat aataacgaat gccagtagca taaatccgca aggtactaag gcaattgatg
    14641 tgcggatgcc cataataact tccggcgttt gcacctgatt ggcgatatat ccgcttaatc
    14701 ccaaaataaa ggcaggaatt gaacctccga ttgcctgacc gcatttacgg gtaaatgaga
    14761 atagtgaata ggtgagccct tcaattcgca cgccggtcag gtattcaccg tattctacgg
    14821 tatcagcttc cagtgcccac atcacagtca tagtaacgcc ctgaccaatt gaagcgatgg
    14881 ccaacgcaac caacgccacc ggcagtgacc agacggaaac ccagaagaat agcagataac
    14941 cgcaggttcc cagcaaagcg ccaatcagga aggtattctt tttaccgatc ctcgcgacca
    15001 tccccggcac cagcggtgcc gatgccacag taccaaccag gttttgcacc agtaccagta
    15061 cagtgaacag cccggtatca tttaacacat agcgcacgta gaacaacgac gaggcgctga
    15121 ccgcgaaggt cgaaatcagc acacacagcg caccgatgca caacataaac agcgggcgat
    15181 tccgtttcag ggtttgcaga ctgatcttca atgacggctg cgccacgata cgtaccacat
    15241 tctcacgcgt cgatttgaag cagatgaagt aaagcaccat tccggcaatc gccagcacaa
    15301 tcgtccagaa atggtatacc gacaccatct cttccgggct ggagttctta atgctcggtc
    15361 ctatcagaaa tgccaggcag acaaaggtta atgaagcggc aatcccgcga gccgcgccca
    15421 gacgggcgcg ggattgtggt tgttgggtca tcgcggtagc aagtgaacca taaggaatat
    15481 tcaccaggct gtagcaaagc ccgaggccca tgtaggtcaa atatgcatac actactttgc
    15541 tgctatggct ccagtcggtc ggtacccaga ataccagcac gctgaagatc attaacggcg
    15601 cagtaccgaa gagtaaaaac gggcggaatt ttccccagcg ggtattcaca ctgtccacca
    15661 ctcgtccggc aaagacgtcg gcgaaggcat cgaatacccg caccagtaac agcatggtgc
    15721 ccgccgcagc ggcaccgacg ccagcgacgt cggtgtagta actcaacagg aagagcgccc
    15781 ccattgcgaa ggcgaagtta ttggcgacgt caccgaggct gtagccgacg atggtgcgcc
    15841 aggagagttg ttgattcatt gtttgcctcc ctgctgcggt ttttcaccga agttcatgcc
    15901 agtccagcgt ttttgcagca gaaaagccgc cgacttcggt ttgcggtcgc gagtgaagat
    15961 ccctttcttg ttacctccaa cgcgcaatat gccttgcgag gtcgcaaaat cggcgaaatt
    16021 ccatacctgt tcaccgacga cggcgctgac gcgatcaaag atgcggtgat acatatccag
    16081 ccatgcacac tgatactctt cactccacat gtcggtgtac attgagtgca gcccggctaa
    16141 cgtatccacg ccgtattcgg tgatgataat cggctgatgc agtttctcct gccaggccag
    16201 aagttctttt tccagtacct tctctgccgt ttccaaatcg ccgctttgga cataccatcc
    16261 gtaataacgg ttcaggcaca gcacatcaaa gagatcgctg atggtatcgg tgtgagcgtc
    16321 gcagaacatt acattgacgc aggtgatcgg acgcgtcggg tcgagtttac gcgttgcttc
    16381 cgccagtggc gcgaaatatt cccgtgcacc ttgcggacgg gtatccggtt cgttggcaat
    16441 actccacatc accacgcttg ggtggttttt gtcacgcgct atcagctctt taatcgcctg
    16501 taagtgcgct tgctgagttt ccccgttgac tgcctcttcg ctgtacagtt ctttcggctt
    16561 gttgcccgct tcgaaaccaa tgcctaaaga gaggttaaag ccgacagcag cagtttcatc
    16621 aatcaccacg atgccatgtt catctgccca gtcgagcatc tcttcagcgt aagggtaatg
    16681 cgaggtacgg taggagttgg ccccaatcca gtccattaat gcgtggtcgt gcaccatcag
    16741 cacgttatcg aatcctttgc cacgcaagtc cgcatcttca tgacgaccaa agccagtaaa
    16801 gtagaacggt ttgtggttaa tcaggaactg ttcgcccttc actgccactg accggatgcc
    16861 gacgcgaagc gggtagatat cacactctgt ctggcttttg gctgtgacgc acagttcata
    16921 gagataacct tcacccggtt gccagaggtg cggattcacc acttgcaaag tcccgctagt
    16981 gccttgtcca gttgcaacca cctgttgatc cgcatcacgc agttcaacgc tgacatcacc
    17041 attggccacc acctgccagt caacagacgc gtggttacag tcttgcgcga catgcgtcac
    17101 cacggtgata tcgtccaccc aggtgttcgg cgtggtgtag agcattacgc tgcgatggat
    17161 cccggcatag ttaaagaaat catggaagta agactgcttt ttcttgccgt tttcgtcggt
    17221 aatcaccatt cccggcggga tagtctgcca gttcagttcg ttgttcacac aaacggtgat
    17281 acgtacactt ttcccggcaa taacatacgg cgtgacatcg gcttcaaatg gcgtatagcc
    17341 gccctgatgc tccatcactt cctgattatt gacccacact ttgccgtaat gagtgaccgc
    17401 atcgaaacgc agcacgatac gctggcctgc ccaacctttc ggtataaaga cttcgcgctg
    17461 ataccagacg ttgcccgcat aattacgaat atctgcatcg gcgaactgat cgttaaaact
    17521 gcctggcaca gcaattgccc ggctttcttg taacgcgctt tcccaccaac gctgatcaat
    17581 tccacagttt tcgcgatcca gactgaatgc ccacaggccg tcgagttttt tgatttcacg
    17641 ggttggggtt tctacaggac gtaacataag ggactcctca ttaagataaa aatactggtc
    17701 aacctttaat ctgattagtt aaccaattct gtgttgcaca cagttatctg tgagaaaccc
    17761 atggaagaga aatggaaata aaaagaactg acaccgcgat caaaaaacca aaaactgcaa
    17821 tacccacagc gtgatattga tcgcattaat ctttaaaacg gcgttgacag aagccataaa
    17881 ataagcaacc gaacgttcgt tcgagtatat tgggaacgat aaactctcat gacatatggc
    17941 tacggtgaat atttttgggg gggaattctc cgatcccggc tagattgtat tttgtacagg
    18001 gaaattaaag tatgaaacgc gggcacagtt atcaccagcc cgcgaaatga gaggatcagg
    18061 atgcggttaa gataccgcca atcatcgcgc gtaatccctg agcaaacgac gcttcacgcg
    18121 ccagatcttc cgcttcgata tcggccagta caccgtaggt cagatccagc agtaaacgtg
    18181 aagtcattga cgtgttgatg tctgggctta tctcgccttt ttgctgggca tcacgcatcc
    18241 gctgggcaac aaattcagtg atcgtcatat gcttattttt aagcatggcg gcgacctgtg
    18301 ggttacgcat cccttccgcc ataatttcga ccaccagcgc ccgttgccca aaggcttcat
    18361 gggtgagaga gacaatagac tcgaccatat agtcaacaaa atgaatccct tcaatcggtt
    18421 cccggaaacg ggccagcgcc ctctcctggt cctgtaagat aatcgcctga atcaaggctt
    18481 ctttggagat gaaatggtga tagagcgtcc cgggactaat ggcgcaggat ttacagatgg
    18541 ctttcatcga ggcactgtga aatccatttt ctgaaaaaat ctctctggca gcattgagga
    18601 tccgggtccg tgtcggttgt gcttcagtct gcatgttatc catcattaaa ccattatagt
    18661 tagcgtgaat aactagttta ccgcgttgtc aggtgagcgc ctatcattgc tgttgaatag
    18721 tgcgaaggca cactctattc gtatgctttt catccagcta ttggttaacc aatagccgtg
    18781 atttggctgc cagttgttta gcagagacgt tgatgaattg cacacgctat cccggtaaac
    18841 aacacatacg gctgtttacc ggttcgttag tgtattaatt gagctcctgt accccaccac
    18901 cggagacggt gagaatttgt ccgcttaccc agctggctgc aggcgagcaa aggaacagcg
    18961 ccgcgttagc aatatcttgc ggttggccca gacgtctgat cggcgtgtgc tgtaacattt
    19021 tttgttcaat ttctggtgta ataacggatt tcagggcatc ggttaatatt gcccccggcg
    19081 caatgccatt cacccgaata tttttttcac ccaggtcaaa tgccatattt ctgaccagat
    19141 gactggccgc agctttagat gatgcatagg aagtcatgtt tatattttta ttttctgccg
    19201 ccatagaagt gatggtcaga ataacgccac cgccattttt ttccatttct ggcgcaacaa
    19261 gttgtgacag atggaaaaaa gaaaacacat tcagttcata agcacggcga aaatctgcca
    19321 ttggcatatc aaacggttta ggaccaccgc caccggcgtt gttaaccagg atatcaactt
    19381 tacccagctt actgacggca aagtctgcca gtgcagagag ttcctgttcg gaagtaatat
    19441 cacaacggca ggcaaatgcc tgaccaccca gttgttgaat ttcgtctaca acatggttag
    19501 ctgcgtcggc gttaatatca ctgaccacca cagatgcgcc agctgtcgcg aatgtaatgg
    19561 cgatttcttt accaatacct gcacccgcac ctgtgatgat ggcgcatttt ccgtcgagtc
    19621 tcaggttgtc agaattaaac acataaacct cctgtgaact tcagtaaaaa taaaaggcac
    19681 cgcctgtgtg ccacattttt tatcattaac cttgaggaaa ggacactacc aggtatagca
    19741 gataaagaga ggattatttc gccgcaatga gccgggcggg aataataaga ttgcgtgaat
    19801 gcgtctcttc atgggtgatt ttttgcatca tgcgatccgc aagtgtggta ccaagttccc
    19861 gcgcaggcgt gctggcccag gtaacgggta tatcatcaag tgtggttggt gtcgcatcgg
    19921 taaatgccgc cagcgaaact tgttgctcaa agtaacgatc gactccgctt tccccgcttt
    19981 gccgccctgc tttgagcaaa ccaaaccatg cccccatcgc aatagtttcg ttatagcaaa
    20041 ccacagcact gatggtcggg ttatgacgta aaagcgccgt gatagcttcc gcggcttgct
    20101 tctggctgga tgtgcactcc agcacccaat cgctgtgaaa cggcaggcca aattttagta
    20161 gagttgcaca atagcccccc acccgttctg cacgggttaa tgaggaactt tgccctccca
    20221 gccaggcgat ccgctgatgc ccattgcgaa tgagatgctc cgtcaacaac tgtgcagcct
    20281 gcatgttgtc cgggcgaacc gtatcaacat catcaagata actggcacgg gaagcgaata
    20341 tcaccgggat agctttttct tctgccatcc gtcgcaggtc atcgctgctt cctgcagccc
    20401 cggcaattac cacaccatcc acaccctgat tcagtaacag tgaaaaccgc tgtgccagct
    20461 gctcgccgtc tttaccgccg tgaagcaaaa aaaccatccg tccctgcgct tccagagctt
    20521 ccgtcaatcc ggccgtcaat tcggcgtaaa acggcgcaga taaatcacgg acgatcaaac
    20581 caatgacgcc gctttgcccg ccgcgcagcg ccgacgcctg gcgattgcgc acaaatccca
    20641 gctcttcaat ggcggcgtta acgcgttctc ctgtggcggt agagattcgc cctttgccac
    20701 taagcaccag cgaaacggta cttaccgaca cgcccgcagc cagcgcaaca tcatgaatgg
    20761 ttattttttt ggcggtagcc atgcagatga cctactccct gattatgtga cagataaaac
    20821 gttttacctt ttatttatct tatacccgct attatcgttg cgtaatgtga tttatgcctc
    20881 actaaaattt gataaaacgt tttatcttct cgcgcaattt actgaatcca gattgttctc
    20941 tacgaggagt cgttttatga cggcgaaaac agcagcgaaa gtcacgctgt gggagttctt
    21001 ccagcagtta ggcaaaacct tcatgttacc cgtggcatta ttgtcgttct gcggcattat
    21061 gctcggcatt ggtagttctc ttagcagcca tgatgtcata accctgatcc cggtcctggg
    21121 caaccccgtg ttgcaggcta tctttacctg gatgagtaag attggctcgt ttgcttttag
    21181 tttcctgcct gtcatgttct gtatcgccat cccgctgggc ctggcacgcg aaaataaagg
    21241 cgtagcggca ttcgctggct tcgtcggtta tgcggtaatg aacctcgcgg taaacttctg
    21301 gttgaccaat aaaggcattc tgccaaccac ggatgccgcg gttctgaaag ccaataacat
    21361 ccagagcatt cttgggatcc agtcgatcga taccgggatc ctcggtgcgg tgatcgccgg
    21421 tattatcgtc tggatgctgc atgagcgttt ccataatatc cgcctgccgg atgcgctggc
    21481 attcttcggc ggtacgcgct tcgtaccaat tatctcctcg ctggtgatgg gccttgtcgg
    21541 cctggtgatt ccattagtct ggccgatttt cgccatgggt attagcggct tgggccatat
    21601 gataaacagc gcgggtgatt tcggaccgat gctgtttggt accggtgaac gtctgctgtt
    21661 gccgtttggt ctgcatcaca ttctggtagc attaattcgc tttaccgacg caggcggcac
    21721 gcaggaagtc tgcggtcaaa ccgtcagcgg cgcactgacc atcttccagg cgcaattgag
    21781 ttgcccgacc actcacggtt tttctgaaag cgccacgcgt ttcctttcgc aaggtaaaat
    21841 gcctgcgttt ctcggcggtc tgccgggtgc agcgttagct atgtatcact gcgcgcgccc
    21901 ggaaaatcgc cataaaatta aaggtctgct gatttctggc ctgatcgcct gcgtcgttgg
    21961 cggcactacc gaaccgctgg aattcctgtt cctgttcgta gcgccagttc tgtatgtcat
    22021 ccacgcgctg ttaaccggcc tcggcttcac cgtcatgtct gtgctcggcg tcaccatcgg
    22081 taataccgac ggcaatatca tcgacttcgt ggtgttcggt attttgcatg gtctgtcaac
    22141 caagtggtac atggtgccag tggtggcggc aatctggttt gtcgtttact acgtcatctt
    22201 ccgtttcgct atcacccgct tcaatctgaa aaccccgggg cgcgatagcg aagttgccag
    22261 ctcaatcgaa aaagccgttg ccggtgcgcc gggtaaatca ggttacaacg ttcctgcaat
    22321 cctcgaagca ttaggcggtg ccgacaatat tgtcagcctc gataactgca ttacccgtct
    22381 gcgtttgtct gtgaaagata tgtcgcttgt taatgtgcag gcactgaagg acaatcgggc
    22441 aattggcgta gtacaactta atcaacataa cctgcaggtt gttatcgggc cacaagttca
    22501 gtcagtaaaa gatgaaatgg ccggtctgat gcatactgtc caggcataag gataagatat
    22561 gttcgatttt tcaaaggtcg tggatcgtca tggcacatgg tgtacacagt gggattatgt
    22621 cgctgaccgt ttcggcactg ctgacctgtt accgttcacg atttcagaca tggattttgc
    22681 cactgccccc tgcattatcg aggcgctgaa tcagcgcctg atgcacggcg tatttggcta
    22741 cagccgctgg aaaaacgatg agtttctcgc ggctattgcc cactggtttt ccacccagca
    22801 ttacaccgca attgatacgc agacggtggt gtatggccct tctgtcatct atatggtttc
    22861 agaactgatt cgtcagtggt ctgaaacagg tgaaggcgtg gtgatccaca cgcccgccta
    22921 tgacgcattt tacaaggcca ttgaaggtaa ccagcgcaca gtaatgcccg ttgctttaga
    22981 gaagcaggct gacgactggt tttgcgatat gggcaagctg gaagccgtgt tggcgaaacc
    23041 agaatgtaaa attatgctcc tgtgtagccc acagaatcct accgggaaag tgtggacgtg
    23101 cgatgagctg gagatcatgg ctgacctgtg cgagcgtcat ggtgtgcggg ttatttccga
    23161 tgaaatccat atggatatgg tctggggcga gcagccgcat attccctgga gtaatgtggc
    23221 gcggggagac tgggcgttgc tgacgtcggg ctcgaaaagt ttcaatattc ccgcgctgac
    23281 cggtgcttac gggattatag aaaatagcag cagccgtgat gcctatttat cggcactgaa
    23341 aggacgtgat gggctttctt ccccttcggt actggcgtta actgcccata tcgccgccta
    23401 tcagcaaggt gcaccgtggc tggatgcctt acgcgtctat ctgaaagata acctgacgta
    23461 tatctcagat aaaatgaacg ccgcgtttcc tgaactcaac tggcagatcc cgcaatccac
    23521 ttatctggcc tggcttgatc tacgtccgtt gaatattgac gacaacgcgt tgcaaaaagc
    23581 gcttatcgag caagaaaaag tcgcgatcat gccggggtat acctatggtg aagaaggtcg
    23641 tggttttgtc cgtctcaatg ccggctgccc acgttcgaaa ctggaaaaag gtgtggctgg
    23701 attaataaac gccatccgcg ctgttcgcta accccaattg cgcaacgtaa aaaatcgttg
    23761 cgcaatcgtg gatttttacc ctgctttgtt tttataatgg tgcgcacttt tatattcaga
    23821 aaaagagtgc gaccatgatt gataccaccc tgccattaac tgatatccat cgccaccttg
    23881 atggcaacat tcgtccccag accattcttg aacttggccg ccagtataat atctcgcttc
    23941 ctgcacaatc cctggaaaca ctgattcccc acgttcaggt cattgccaac gaacccgatc
    24001 tggtgagctt tctgaccaaa cttgactggg gcgttaaagt tctcgcctct cttgatgcct
    24061 gtcgccgcgt ggcatttgaa aacattgaag atgcagcccg tcacggcctg cactatgtcg
    24121 agctgcgttt ttcaccaggc tacatggcaa tggcacatca gctgcctgta gcgggtgttg
    24181 tcgaagcggt gatcgatggc gtacgtgaag gttgccgcac ctttggtgtg caggcgaagc
    24241 ttatcggcat tatgagccgg accttcggcg aagccgcctg tcagcaagag ctggaggcct
    24301 ttttagccca ccgtgaccag attaccgcac ttgatttagc cggtgatgaa cttggtttcc
    24361 cgggaagtct gttcctttct cacttcaacc gcgcgcgtga tgcgggctgg catattaccg
    24421 tccatgcagg cgaagctgcc gggccggaaa gcatctggca ggcgattcgt gaactgggtg
    24481 cggagcgtat tggacatggc gtaaaagcca ttgaagatcg ggcgctgatg gattttctcg
    24541 ccgagcaaca aattggtatt gaatcctgtc tgacctccaa tattcagacc agcaccgtag
    24601 cagagctggc tgcacatccg ctgaaaacgt tccttgagca tggcattcgt gccagcatta
    24661 acactgacga tcccggcgta cagggagtgg atatcattca cgaatatacc gttgccgcgc
    24721 cagctgctgg gttatcccgc gagcaaatcc gccaggcaca gattaatggt ctggaaatgg
    24781 ctttcctcag cgctgaggaa aaacgcgcac tgcgagaaaa agtcgccgcg aagtaacaaa
    24841 atggatggtg caaattgcac catccatttt tcatgcaagg cacaaagtcg cgcgatgttt
    24901 ggcggattcg atgcccagct caatcaactc cattacctgg attgcctggc ttgccggaac
    24961 cggattttca ccatcgccat ttaacgcatc acgaatagcc gcatagtaag ccggataatt
    25021 cccaggcacc gtcaacagcg tttcttcgac acgttcctca ccttccacgc gggtaagtac
    25081 gccatcacgc atatcgtagc cccagtcttc ctgcggtaga cgctcgccat ttttcagacg
    25141 ttcttcctgt ggatcgaggc catatttcac ataactgcct cgggatccat gcacgatata
    25201 ccgtgctgac tcagcggctg ccagcatggt accgtgtaaa atgactcgcc gctgtggata
    25261 ggacaagatg gcgtggaaat aatcggtcga ctgcgctccg ggccgtaact gtgccaaatc
    25321 taccgtcatg ctgaccggta aaccaaaaag cgtaatggcc tgatcaagaa gatgtggtgc
    25381 taaatcgtac cagataccgc tgcctgggcc gccctgttca cgccaacgat cgcgcacctg
    25441 cggacggaag cggtcaaaat gagactcaaa gtaagcaact tcacccagca cgccttccgc
    25501 gagtaaacct tttagcgtca agaaatcgct atcccagcga cggttatgga atacagacag
    25561 cacacgcccc aggctttttg ccagcgcatc cagctctcgc gcttgtgaca gtgtcacggt
    25621 aaagggttta tcaacgacca catgtttacc cgcctcaagc gccgctttgg ctaacgggaa
    25681 atgggtatcg ttgggtgtag gaatgacaat caggtctatg ttgggatcgt taaacagatg
    25741 cttcggctca gagacaaccg taaccgttgg ccagtcggct tttacttttg tttcatcact
    25801 gctggagatt accgccagtt ccagcccggg cgtgcccgca atcaggggcg catggaaggt
    25861 tttgctcgca taaccatacc caatcaaccc aacacggatg ttgtcgctca tgtcattgcc
    25921 tctcattgaa gtatgatggc tatttgacac tatcctttac ccacgctcaa cagtttaata
    25981 acctgccagc aataagggat gttgtttaac ttaagtcaaa aaaatagcga attttccaac
    26041 gacaaaagct aaatatcgca aaaacctcag taaaaatctt gctggagcta ttattgctaa
    26101 gtaacattta ccccctgaag ttaatggatc aatcaagaga gatgtgggct gtaatgaatc
    26161 gtcttattga attaacaggt tggatcgttc ttgtcgtttc agtcattctt cttggcgtgg
    26221 cgagtcacat tgacaactat cagccacctg aacagagtgc ttcggtacaa cacaagtaag
    26281 ctctgcactt gtggagcgac atgctgcccg tccgggtgca tgttttcact tgtcggatat
    26341 taaaccagga atttattatc ttgttcgatg ttgttggtga ttgtcaggga tagtaaagcg
    26401 cgttactctt ttcagagcag tttttcatcc ctgaatattt gccatcagcg cattcttatt
    26461 attgtaataa cacaagcgtt cacttttggt gaaattttat catcactaat aatacctacc
    26521 cctttggtat taattatttt atggaccttt tatgactgtt caggactact tattaaaatt
    26581 tcgcaaaatc agttcactcg aaagtctgga aaaactctac gaccatctta attacaccct
    26641 gacggacgat caggaactga tcaatatgta tcgtgctgcc gatcaccgtc gcgcagagct
    26701 ggtttctggc gggcgtttgt ttgacctcgg ccaggtaccg aagtccgtct ggcactatgt
    26761 ccaataaaga aagtagcgat cggttataaa agccttataa taacgcccct aaaattagat
    26821 ttgcctgttt gcgtcgctat tctggagaac ctatgaccac cacaacgcca caacgtattg
    26881 gaggctggtt gcttggcccg ttggcctggc ttttagtcgc tctgttgagt acgacgctgg
    26941 cgttgttgct gtataccgct gcgttatctt ctccgcaaac atttcaaacg ctcggcggac
    27001 aagcactgac gacacaaatc ttatggggcg tttcttttat taccgctatc gccatgtggt
    27061 attacacact gtggttgacc atcgctttct ttaaacgtcg gcgttgtgtt cctaaacact
    27121 atattatctg gctgcttatt tccgtattgc tggcagtaaa agcattcgct ttttcacccg
    27181 tggaagatgg catagccgtt cgccagttgc tgtttacttt gttggcaacc gcattgatcg
    27241 tcccctattt caaacgctcg tcgcgggtaa aagcgacgtt tgtgaatccg taataacctt
    27301 acagttaacc tgttgtcgcc tgctctggat taacggataa taggcggctt ttttatttca
    27361 ggccgaaaaa tgactgacta cctgttactg tttgtcggaa ctgtactggt caataacttt
    27421 gtactggtca agtttctcgg tctctgtccg tttatggggg tttccaaaaa actggaaacc
    27481 gcgatgggca tggggctggc aacaacgttt gtgatgacgc tggcgtctat ttgcgcctgg
    27541 cttatcgata cgtggatttt gatcccactt aatctgattt acctgcgcac cctggcattt
    27601 attctggtga ttgctgtggt cgtgcagttc accgagatgg tggtgcgcaa aaccagcccg
    27661 gtgctttacc gtctgctggg gatttttttg ccgcttatca ccaccaactg tgcagtgctc
    27721 ggcgtggcgt tgctgaatat caatctcggg cacaatttct tgcagtcggc gctgtacggt
    27781 ttttccgccg ctgtcggttt ctcgctggtg atggtgctct tcgccgccat ccgcgaacgc
    27841 cttgctgtgg ctgatgtccc ggcacctttt cgcggtaatg ccattgcgtt aattaccgca
    27901 ggtcttatgt ctctggcctt tatgggcttt agtggtttgg tgaagttgta atgaatgcta
    27961 tctggattgc cgttgccgcc gtgagcctgc tgggcctggc gtttggcgcc attctgggtt
    28021 atgcctcccg ccgttttgcg gtggaagacg atccggtcgt tgagaaaatt gacgaaatct
    28081 taccgcagag ccagtgtggt cagtgcggtt atcccggctg tcgcccctac gcggaagcca
    28141 tcagctgtaa cggtgaaaaa atcaaccgtt gcgccccagg tggcgaagct gtgatgctaa
    28201 aaattgccga gttgcttaat gtcgagccgc agccgctgga tggcgaagcg caagagctaa
    28261 cgcctgcgcg gatggtggcg gttattgatg aaaataactg tattggctgc actaaatgta
    28321 ttcaggcttg tccggtagac gccatcgttg gcgctacccg tgccatgcat acggtaatga
    28381 gtgatctctg tacgggctgc aatttatgtg ttgatccgtg cccgacgcac tgcatctcgt
    28441 tgcaaccggt cgcagaaaca cctgactcct ggaaatggga tctgaacacc attcccgtgc
    28501 gtatcattcc cgtggaacac catgcttaag ttattctctg cattcagaaa aaataaaatc
    28561 tgggatttca acggcggcat ccatccaccg gagatgaaaa cccagtccaa cggtacaccc
    28621 ctgcgccagg taccactggc gcagcgtttt gttattccac tgaaacagca tattggcgct
    28681 gaaggtgagt tgtgcgttag cgtcggcgat aaagtattgc gcggccagcc gcttacccgt
    28741 ggtcgcggca aaatgctgcc tgttcacgcg cccacctcgg gtaccgttac ggctattgcg
    28801 ccccactcta cggctcatcc ttcagcttta gctgaattaa gcgtgattat tgatgccgat
    28861 ggtgaagact gctggatccc gcgcgacggc tgggccgatt atcgcactcg cagtcgcgaa
    28921 gagttaatcg agcgcataca tcagtttggt gtagccgggc tgggcggtgc aggattcccg
    28981 acaggcgtta aattgcaggg tggcggagat aagattgaaa cgttgattat caacgcggct
    29041 gagtgcgagc cgtacattac cgccgatgac cgtttgatgc aggattgcgc ggctcaggtc
    29101 gtagagggta ttcgcattct tgcgcatatt ctgcagccac gcgaaattct tatcggcatt
    29161 gaagataaca aaccgcaggc gatttccatg ctgcgcgcgg tgctggcgga ctctaacgat
    29221 atttctctgc gggtgattcc aaccaaatat ccttctggcg gtgctaaaca attaacctac
    29281 attctgaccg ggaagcaggt tccacatggc gggcgttcat ccgatatcgg cgtattaatg
    29341 caaaacgtcg gcactgctta tgcagtgaaa cgtgccgtta ttgatggcga gccgattacc
    29401 gagcgtgttg taaccctgac tggcgaagca atcgctcgcc cgggcaacgt ctgggcacgg
    29461 ctggggacgc cagtgcgtca tttattgaat gatgccggat tctgcccctc tgccgatcaa
    29521 atggtgatta tgggtggccc gctaatgggc tttaccttgc catggctgga tgtcccggtc
    29581 gtaaagatta ccaactgtct gttggctccc tctgccaatg aacttggcga accacaggaa
    29641 gaacaaagct gcatccggtg tagcgcctgt gctgacgcct gccctgcgga tcttttgccg
    29701 caacagttgt actggttcag caaaggtcag caacacgata aagctaccac gcataacatt
    29761 gctgattgca ttgaatgtgg ggcttgcgcg tgggtttgcc cgagcaatat tcccctggtg
    29821 caatatttcc gtcaggaaaa agctgaaatt gcggctattc gtcaggaaga aaagcgcgcc
    29881 gcagaagcca aagcgcgttt cgaagcgcgc caggctcgtc tggagcgcga aaaagcggct
    29941 cgccttgaac gacataagag cgcagccgtt caacctgcag ccaaagataa agatgcgatt
    30001 gctgccgctc tggcgcgggt gaaagagaaa caggcccagg ctacacagcc tattgtgatt
    30061 aaagcgggcg aacgcccgga taacagtgca attattgcag cacgggaagc ccgtaaagcg
    30121 caagccagag cgaaacaggc agaactgcag caaactaacg acgcagcaac cgttgctgat
    30181 ccacgtaaaa ctgccgttga agcagctatc gcccg
//
LOCUS       DAZGVQ010000048        30173 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000048 DAZGVQ010000000
VERSION     DAZGVQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.048"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..329)
                     /locus_tag="ACHLAG_002063"
     CDS             complement(<1..329)
                     /locus_tag="ACHLAG_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655302.1"
                     /translation="MELTADLLKSLNNIRQNATIYVHAYHYTDDEKCVMAPRSSLPSL
                     ELTVLQSEKIIDLLLSGYGYIRHDGYVQGYGEKTVATFTIQEGKHHHFRTNLALNKTG
                     NIADNAL"
     gene            726..971
                     /locus_tag="ACHLAG_002064"
                     /pseudo
     CDS             726..971
                     /locus_tag="ACHLAG_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
     gene            complement(1167..1400)
                     /locus_tag="ACHLAG_002065"
                     /pseudo
     CDS             complement(1167..1400)
                     /locus_tag="ACHLAG_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1700..5557)
                     /gene="sigA"
                     /locus_tag="ACHLAG_002066"
     CDS             complement(1700..5557)
                     /gene="sigA"
                     /locus_tag="ACHLAG_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFP0655303.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYALFNNDRVNNLKNNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVSGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKTAQGNNLKTGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQKHNNNTNLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGS
                     NNCLIPLLCQKDYSAAIRDQESTVNKRYNTEYKSNNQVASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQVHSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQDKEDNLDKTTHIPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNMSPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLIGKYVHHDNEYSATFAGLGTKDYSSHSLYAGAEAGYRYHV
                     TEDAWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPVIGRAGVDVGKSFSGKDW
                     KVTARAGVGYQFDLLNNGETVLRDASGEKRIKGEKDGRMLMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAVNASFRYSF"
     gene            complement(5917..7119)
                     /locus_tag="ACHLAG_002067"
     CDS             complement(5917..7119)
                     /locus_tag="ACHLAG_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFP0655304.1"
                     /translation="MMMKKHITRTLVASAVLFSFNSAAATSYSEARNDAMGGTGVASS
                     HYGVAPLANPALLTKAGPDDDFSLLLPSVGAQLSDPDNITDNADRISDDWKAFDRAID
                     SNHGVPEAAARLKERLRDFRHTHAAAQLGVSAVAALPGDRLSAALMVKSHGTVSVDGK
                     VSDADLTYLEEVANSTGQEVDKSRLTSQAFARAALITDVGIALATELETAGQKWSLGF
                     TPKFQRVDLFNYNVLVRNYDSSAFKGDRYHNTKNGINADIGASTDLDDNWTLGLVAQN
                     LIPRSIETKEVNGITETFRIRPQVTAGVSWHNAMFTTAFDVDLTPASGFTSDSNRQFA
                     AIGAEFNAWKWAQLRAGYRQNLAGNDGSAFTAGVGISPFDVVHLDVAGLIGTDNTYGA
                     VAQFQFTF"
     gene            complement(7209..8030)
                     /locus_tag="ACHLAG_002068"
     CDS             complement(7209..8030)
                     /locus_tag="ACHLAG_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFP0655305.1"
                     /translation="MKPNSTGIAARMILSLDRARICEALLNNRQLQPTPLQVRYPQGV
                     REALSIMSEQLSLSVSDLTRILVEEALSEMFLPADNSVRRLHDRMEYLMQVHELSPVT
                     MATLLAPWNIRPAVFREPDRLTDYLTGEILASLADWFYLSPEWLNGRVHYPLCHPGDW
                     PETQDAFCRLISEDDNIDIILWHGFPFSGEHAQEYCGVLLRQKKKINNAIIYPVLSLY
                     PTGMNKKKEGWFQMARKMSPDIPVRAVTLSPAQAEYLVTGKILPAELFRIPLSPW"
     gene            complement(8591..9715)
                     /locus_tag="ACHLAG_002069"
     CDS             complement(8591..9715)
                     /locus_tag="ACHLAG_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP0655306.1"
                     /translation="MFRKIKIRKMTLNRALDKYLKTVSIHKKGHLQEFYRVNVIKRHP
                     MAERYMDEITTVDIATYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIASVEWGT
                     CRMNPVELVRKPKISSGRDRRLTSGEERRLSRYFRDKNQQLYVIFHLALETAMRQGEI
                     LTLRWEHLDLQHGVAHLPETKNGLPRDVPLSRKARNYLQILPQQINGNVFSYTSSGFK
                     SAWRTALLDLKIENLHFHDLRHEAISRFFELGTLNVMEVAAISGHRSLNMLKRYTHLR
                     AYQLVSKLDAKRKQTCKIAPYFVPYPATVGNRNGLFIVTLHDFDLETRAETRELAISH
                     ASVLLLRTLAQAAQRGERVPTPGELPANIDARVMICPLTS"
     gene            10386..10625
                     /locus_tag="ACHLAG_002070"
     CDS             10386..10625
                     /locus_tag="ACHLAG_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655307.1"
                     /translation="MYASGGNGAGGGDCANTSNLQVYVNGTFIGMNASNNPSYGKTAF
                     ISFAVPVGASYQIISRPTQNYACGNGVFSVYAYQM"
     gene            complement(10630..11973)
                     /gene="pilV"
                     /locus_tag="ACHLAG_002071"
     CDS             complement(10630..11973)
                     /gene="pilV"
                     /locus_tag="ACHLAG_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFP0655308.1"
                     /translation="MKKFRLDDDKGFILLEILAGLILIGIATPMIYSEIEDWLNEQLY
                     QSAALHADEYNNAIKNYVADKTSSLSSKTITVNELIQQGYLNNGFSRSPFGHSYITGI
                     RKNNLSGRLEALTCTTGGQDIKEDGLRRIAGQINGLGGFMLQNNSVTGAFGGWTDLGS
                     NYQITCNKGHIAMRMAGKDLEESDRLYRYSVPGRPDLNRMHTGIDMNNNSITNINEAS
                     GKNARFSGDVTSNRWLHPNGGGFTMTDSQWIRAVNNKGITTEGEIKGGTVRGQTVTGG
                     TVRSDGRLTAGEYLQLNGIANAGTHCSPDGLVGRDNSGAILQCQSGAWSGFESYPVGS
                     PIPWPSATPPQGYLLMNGQSFSCSRYPQLARAYPGCKLPDLRGVFIRGWDNGKGLDGD
                     RNRQLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAFNYIVKAS"
     gene            complement(11970..12620)
                     /locus_tag="ACHLAG_002072"
     CDS             complement(11970..12620)
                     /locus_tag="ACHLAG_002072"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFP0655309.1"
                     /translation="MSELLPICIILIVLAAILYLAYSAATVYLSQVHNTRPHNAAVCI
                     CCLLAVLSICLNGAYGVDATGLLFLSILTGLLTVMTLTDIAVCRLPRIFTLSLIILGA
                     AFRYSQDSLAHALLNASLWFGMIYLLRRIFLAVKGTEALGLGDVFLIAGIAMWTQAQH
                     TPLIITAAASGAFLFILIFCRRRRQQALPFAPFLCASLYVLTLFPDSVIHTSEIFT"
     gene            complement(12637..13113)
                     /locus_tag="ACHLAG_002073"
     CDS             complement(12637..13113)
                     /locus_tag="ACHLAG_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFP0655310.1"
                     /translation="MKWLYFSVLILFSHTSIASVMNNNHLDSCFIQAGKRYLIAPELL
                     KTIAQQESGLVATAINHNKNKSGKIISTDYGIMQINSSHIPELKKLGVIKDKDELLKN
                     PCLNIHTGAWILAHHFQRCGVNWECLGSYNAGFSKNNTARRMIYARIIHNRYFRKD"
     gene            complement(13172..13708)
                     /locus_tag="ACHLAG_002074"
     CDS             complement(13172..13708)
                     /locus_tag="ACHLAG_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFP0655311.1"
                     /translation="MNFRARLFYYFSLKKEKEEDKGLTLLEIAGALIIIGIVVGLAMS
                     QMSSTMSSSDNQRELKNLQTIATKMKQYKFQGRYTGSDYIKTLTESGSLPADMIAGGN
                     KAKNAWGGDVTIKATTDKYGYTITSNNVPKENCVELINSLRSSSMFTKIMNTTPATVD
                     PVTVCSKDKNNITLETNS"
     gene            complement(13759..14859)
                     /locus_tag="ACHLAG_002075"
     CDS             complement(13759..14859)
                     /locus_tag="ACHLAG_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFP0655312.1"
                     /translation="MRFLEKLNNTDIELTALTVWIARKTFALNRVEFYESLGGMIRDG
                     TPSLRALEFLCNVETDFGTRKGTSGMYFLAKECIASIKSSGMLSGALPTWVPPEEATL
                     IRNGEERGNIADALFQVARTVKARREMTSSLIAVCLYPLLLLSLCVVNMYNAHTRIIP
                     IVSAFATEDKWSFQMKVLAGMSEFIIGYGLWLCGAIIALTVLIRFSFSRYTGPGRRML
                     DRVPPWSLYQTLHGVNYLFSISSMLQINIPLSQALLRMEKGAEKNKWLKERIQAIRKH
                     VLSGQNLATAMRNSGYDFPSRVCINKLLLNSEREDSVAAMAVYADQWLEEAKKRVKRS
                     GLIITGISGGFVFFFILNMISAIYSISDMLKQ"
     gene            complement(14861..16381)
                     /locus_tag="ACHLAG_002076"
     CDS             complement(14861..16381)
                     /locus_tag="ACHLAG_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFP0655313.1"
                     /translation="MPETIVLPPEDIRDFVAIQHDADGKIRVKVVEAKKHITSVQRYL
                     TLLRSRYPGKISKFDFTTLDVIAALNSNNAERAPGVLSDGGINTVQRKVLDYIRKAAQ
                     LDASDLHITPGRDNTDFTYVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQ
                     FDPGVPQDARLSEQYLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQQIPTFSMLGYH
                     PDQERTLRRILQRPEGIITLSGPTGSGKSTTLRTASAAYLEQYGFNNTGGILLPRRRL
                     FTIESPPEGRIPGAIQTAVMDTAQGWVDSIKSALRLDPDGILNGEIRDHDSAITAIKA
                     AMTGHLMLTTIHANDPINILERLEMEGVQTRMIADPQLFIGLLSQRLVQLICPHCRRP
                     WHEVATGRTDDERRLVEGFCNPDQVYLRNHEGCSHCWRGVTGRTVIAEVISPDARFFQ
                     LYREKGRIEAKTYWHRELGGMTRNQHLLGKINSGQVDPLAAHYISPIDEDSYTLLH"
     gene            complement(16391..16852)
                     /gene="pilP"
                     /locus_tag="ACHLAG_002077"
     CDS             complement(16391..16852)
                     /gene="pilP"
                     /locus_tag="ACHLAG_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFP0655314.1"
                     /translation="MHDKTKALLIAALLLPAMAVAGNENDASQNKQLQIEQIQEETAL
                     YEAMLAREKIRHELRTLEQGISPVSGSVTGHQPENAAGTSSGSQALSSPRLKEIFGIH
                     PQLSARVILPGGTETDVRAGQALPGTLFMVEQITPDSVILEYNGNRQTLRP"
     gene            complement(16842..18137)
                     /gene="pilO2"
                     /locus_tag="ACHLAG_002078"
     CDS             complement(16842..18137)
                     /gene="pilO2"
                     /locus_tag="ACHLAG_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFP0655315.1"
                     /translation="MQKKIRPVSLISGKQAFLAGLDWKNIPPGYRNARRFARSQGAEL
                     YLSCHNHDGENGDEMMIALLSRQDADIPRNIRQCLSLAMLIRPLLKSGGYAIFIIGND
                     ENDDAELTCAFVSVINGILVNDVTGTPTEITEARNTFLMLNAEPENGWVRYEPDGFSP
                     DSRCIPLPLSNLISTRKHPAEARLLPVSRGPQLMTALLIITLLGASWYGWQRYEIWQA
                     EKAEREESARKAAEARITPPWTGQPETGEFIRQCSTTWNQTPLSAAGWRYTLAECSTD
                     GNSGNLRASYTNTAGTTVTAFIRRITELTDTRPFIVMPEGNSGGVALPVTFRLPDNPV
                     PVDSLPETSDLQERLTTLAQSVQLQIDWQEVNNSFTDENGNIIQPPWKEYDLQIQTLL
                     PANQLAERFSEPSVRFLSVTRQLENGRFRYQFTGKYYAR"
     gene            complement(18161..19762)
                     /locus_tag="ACHLAG_002079"
     CDS             complement(18161..19762)
                     /locus_tag="ACHLAG_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFP0655316.1"
                     /translation="MKKFFCAVTALSVVLSGCSSFERIRATEKNASDEITRAERIAGE
                     LRKQRAVVRETNQQWINTTPLPEKTVTRAIPDCPVVINTREAITLHQIAQRISESCHI
                     PVQIMSDVWTLLGTGENRTQRISGDIPPPDTSGMQPLSTVGLSSAAPSTASLPELNVQ
                     GLPALLNTVSSRFGISWRYVKGKIEFHWLDTRTFPVTYMDSQVAYNARVVSGTMSSTG
                     SSGGSSGSSLSGDASNTQTTAVDMKSTLYEDTKNAVNSMLTPGIGRMVLSTGFLTVTD
                     TPQVLDTVKNFMEERNREMRRQVVLNIEILSIKKTSKEQAGIDWNAVFSDGHLGIDLT
                     SAFTNAADEVVTSGVSIIDGKLTGSKAFLKALSSQGKVSVVTQNSAVTKNLTPVPMQV
                     ASQQGYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFIQPGSHRLQLLFSMSLSD
                     KPVFEVFESGGSRAQTPSVELKTINQTIDLKSDQTVILSGFQQFSRKSLRQGVGTPSF
                     FGLGGGVDSADDDTILVVLITPHVI"
     gene            complement(20012..20446)
                     /gene="pilM"
                     /locus_tag="ACHLAG_002080"
     CDS             complement(20012..20446)
                     /gene="pilM"
                     /locus_tag="ACHLAG_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFP0655317.1"
                     /translation="MGWLMPLIIALFIIGNWFHAGNTERAIKNTDEQQRQQQVYEILR
                     LTNAINDWKYHHPGDIREGKMLTVNETGFAPVYGTKHIISGQRTFVWLPFQPGLISAL
                     NKVSRNSALIAQVRDRKLLDNQGNNIGVPPSVIPENAIVLIN"
     gene            complement(20447..21118)
                     /locus_tag="ACHLAG_002081"
     CDS             complement(20447..21118)
                     /locus_tag="ACHLAG_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001023520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin co-regulated pilus biosynthesis Q family
                     protein"
                     /protein_id="HFP0655318.1"
                     /translation="MNILRYLSISIFAFLFSYTATASTQTSSSNNHAEKRAIGTLKFI
                     RADGHRPFGQAMQQLLPTGWRTEIARDVRLPARISWSGNDQWNHVIEKTLPRYSLKAT
                     FDTSGKRVLIQQAEKTVKSGLFSAPVTGVKKTETRHIEQGMTLRTGLEKWAAAEPCAT
                     GKWKVAWKTPVNYSIDARLVFSGNFMDALNSTFALYQQAEKPLYASVNKNQCVLIITD
                     IPGDS"
     gene            complement(21618..21848)
                     /locus_tag="ACHLAG_002082"
     CDS             complement(21618..21848)
                     /locus_tag="ACHLAG_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000135398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFP0655319.1"
                     /translation="MASAQATPFPYKYCIQATIEKPGGTPVSWIRFQNTPMTKQECEK
                     LFSTEKEAGKSFTVKVGVTQFSCKENPPAPGE"
     gene            22386..23894
                     /locus_tag="ACHLAG_002083"
     CDS             22386..23894
                     /locus_tag="ACHLAG_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFP0655320.1"
                     /translation="MPHKPTEEQTAIIHWQGRKLVVNAFAGTGKTSTLVSYAMACPES
                     RMLYLAYNRAIRDEAERRFPFNVECKTSHQLAFARFGRHYQKRLVPGLFVTDVARKLN
                     TRYWALARVAMTGLNQFIGSADATPGRQHLPDKDEMKGVAPDEALRAVRTLWQAMSDP
                     DGTFPVTHDTYLKLFQLSGPDLSRRWDTILFDEAQDANPVTSALVLGQRCRIVLVGDR
                     YQQIYRFRGADDALSHPSLAGADRLWMTQSFRFGPAVAAMANLLLKRAGESWEVSGCG
                     GDDEVLTMQEMRERIKHGKFGGHRAVLNRTVAGVIGTALMAAMAGRKVYWVGGIEGYR
                     TEALEDLYWFQMDMPARMHSDRLRRDYRNFDEYRHIAKVTKDVEMNQSIRLLELCFPL
                     PKKLELLREHTVTNEREADITVSTAHRSKGLEWERVVLGDDFQDIADPLMSEQERRDE
                     TNLLYVAVTRARRTLVLNELMQQLAEEKSRTKEKTTSPHERGTEKAVEGKDV"
     gene            23887..25392
                     /locus_tag="ACHLAG_002084"
     CDS             23887..25392
                     /locus_tag="ACHLAG_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFP0655321.1"
                     /translation="MFKLRKFFTSGSAVEIPDDGRSVPGTPEGFYAPLTTDSLLMPKN
                     RAQCLKQIRDNNMMPKPLCEAMCCAPVYSLLLSTQNVPAAREGRWSHAGGFGDLTLLH
                     TSYAVRLARAHMFPPGASAEEQAAQGAAWHAVIFWSALFYHLPLLAHLEGELFSGRGW
                     QPGISVPDEAYRFRFRQLPPQGTEAQQLAAVIAGGLLPEGAAAWLATVPGALQNLAGA
                     FWHQHPEMALIRDVLQESARQAESPLLNGGIVATASHIASAEVVTGEVVVSGKTESTV
                     APAVSGEEAAAEGNTVSGNAAESVQEQPEMRQQQAEKPTTPDSSEGSEDDAACEMESI
                     PTDEDTAMLVSMFAEEARAPEKGEDDSPSGEVVAPVSSTGEISAAGDDEEDFIAWLKM
                     GLSSGMIEANTPSARVHYIAGYVFLSVPDIFFLYRKLTGSQRERKNIQALFERACIHK
                     IREGERFFSVRLYSVPEKTGNYKTVSGYLVKGRYLYGYNKVPEDSAFIIFP"
     gene            25431..26528
                     /locus_tag="ACHLAG_002085"
     CDS             25431..26528
                     /locus_tag="ACHLAG_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0655322.1"
                     /translation="MVSDEKMSWEELLEEYFLIKNLKPDTEHSYRKVVRLFNEFMGGN
                     SWPGDVTARDIQRWRRHMLKVQGRSTNTWNNRVSHLRPILALGIRKKWLPQTENPLDD
                     ASVPGESKKKKTLTEKQMIKVYLTVQNFAEAEVKRQVVCDRMRNAMYPAWYWLTVLDV
                     LRFTGMRFNQLKHIRLKDVHEDEAVIALQLEGSKTRREWSVPIVTPLQISLKHLLACA
                     RANGAMDDDYLFDVCLYSEHYGPGKYKYCARRAHQSVRSFFRRLSRECGFNVSPHRFR
                     HTLATKLMEAPERNLHMVKSILGHRSVATTMEYVDLSLKVAAQTLEAELSLSTDEGTK
                     RRIHEARKHSYGYSEVEQSSDVSEVESTDTE"
     gene            complement(26734..26809)
                     /locus_tag="ACHLAG_002086"
     tRNA            complement(26734..26809)
                     /locus_tag="ACHLAG_002086"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26774..26776),aa:Phe,seq:gaa)
     gene            complement(26915..27613)
                     /gene="yqgA"
                     /locus_tag="ACHLAG_002087"
     CDS             complement(26915..27613)
                     /gene="yqgA"
                     /locus_tag="ACHLAG_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFP0655323.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWFA"
     gene            27766..27849
                     /gene="yqgH"
                     /locus_tag="ACHLAG_002088"
     CDS             27766..27849
                     /gene="yqgH"
                     /locus_tag="ACHLAG_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFP0655324.1"
                     /translation="MHAISLHSLAYRHFARNVSLSMLLLMR"
     gene            28011..30146
                     /gene="speC"
                     /locus_tag="ACHLAG_002089"
     CDS             28011..30146
                     /gene="speC"
                     /locus_tag="ACHLAG_002089"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFP0655325.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
BASE COUNT         7183 a         6980 c         7879 g         8131 t
ORIGIN      
        1 ttcaacgcat tatcggctat attgcccgtc ttattcagtg ccagatttgt cctgaaatga
       61 tggtgcttac cctcctgtat tgtgaaagtt gccacagttt tttctccata tccctgaaca
      121 tagccgtcat gcctgatata accgtaacca ctcagaagca gatcgataat tttttctgac
      181 tgcagaactg ttagctccag agagggcagg gatgagcgtg gtgccataac gcatttttca
      241 tcatcagtat agtggtatgc gtgaacatat atggtggcat tttgtcttat attgttaagg
      301 cttttcagca ggtctgcagt caactccatc gcggtgtcct tccggttgtt gtcatctgtt
      361 gatgatgcga cctgttgaaa gcgttggcga caattaattc tttttctctt ctgagaaatt
      421 tacagaatac aaaaagagtt atttgttcac gacctgttgc tttctgacga tattccctgc
      481 ttcatttgtt gaagcaggag aatattgatg gtaaagacac atccatcttc tgtaagacgt
      541 gtcgcagttt atgggctggc tggattgcag tttctttttt caggctacct caagtcgtta
      601 ttcctgataa ccggttgctt aatctgaacc gctggcggag ggtgtaaccc gaccgccatt
      661 caccccgtta gggcattgtt cctcacggga cagtgccctc ttcatttacc agaggagcat
      721 tgattatggc tgaatggtgc agaaatcgtt ttgaaatcac cggtaagtca gtctgtcttg
      781 acgtactgat tcagtggatt gaagggaatg atgcatcccg gtaccgtcat gcgatccagc
      841 agagcatact gctgtttctt gcaggctgtg cggggatact gaaaccggta agaacggaga
      901 catatccgcc ttatccaggg ctggtcagtc acggtgccgg ctcaacaact gtagccagtc
      961 aggcatggga ggtcgcggag catgagcagc cacaactctg tctgatcagg gacacacagc
     1021 caggcgaacg cgccatggca tcagcatcag tcgggtcccc ggggccagta gtaaaaatag
     1081 tgtaatgaaa caatgcgcca cattttgtgg cgctttttta ataagtcagt gatggcagag
     1141 ttgttattgt aaataactct ccgtgtttac tgggccagtt tttttatcag gtcgtgaatt
     1201 ttatcacctg aaaactgggt catgaaagtt atctttatgg atttactgat ttgttcgaag
     1261 agtttctgtg cgtgttttat tttatcatgc tcttcatgtc tgagttcgcg tttgccatca
     1321 acatttttgg tttcaataat aaggttcaga taatcaccct ttgacgtgtt tacaacataa
     1381 gcgaagtcag ttatggggct tgccggcgtt ctccgggggg aaagctccgt gctactgtca
     1441 cctggaaagc cgttgccgca agcttgctcc tcgtaagtgt taacggagcg ccgtattgac
     1501 gcttattcac tggtgagtac tacgttccat gggggggcgt atgcgccgcc gatgacgcgc
     1561 ccgaaaaacg cgtcaatatg agtccgccgg aacgatacag agtttctgca agtaaaacac
     1621 ggtgttctcc ggagcacgct attttatatt tgttttattg aatataagag ggggccataa
     1681 agcggccccc ttgtttttat cagaaagagt aacggaagct ggcgttcacc gcgttatcca
     1741 cgttgtactt accaaatgcc gatttctcaa actccagacc aaagcggaga ttatcgcgaa
     1801 tctcagcatt cagaccaaca ttcatgagca tgcgtccgtc tttctcgcct ttgattcgtt
     1861 tctcaccaga ggcgtcccgc agaacggttt caccgttatt cagcaggtca aactgataac
     1921 cgacaccggc acgggcagtc actttccagt ccttaccgga gaacgattta cccacatcca
     1981 caccggcacg accaatcact ggattgaagt ccttgtcttt catggtcacg ctcattccgc
     2041 gatcctgcca gtcgaaccgt ttacctgata cggccccata aaccagttcc gcctgcggct
     2101 caatccaggc gtcttctgtt acatgatagc ggtagcctgc ttcagcacct gcatacaagg
     2161 aatgagaact gtagtctttt gttccgagtc cggcaaaggt cgccgagtac tcattatcat
     2221 ggtgaacata cttaccaatc aggtcgatat aggcaccaga gtcaaatata gcggaagcat
     2281 acagacctgc cccgacggac ttcgtcttgc cactgaatgc attactgctg gcatgactgt
     2341 cggtatacgt catagtcaga ccagtgaaaa ggtcaagtcc atccagctca tgttttttat
     2401 ccacaccaat ctgcacatgg gtgtagttgt cactgtatcc accagaagct gaacctgctc
     2461 cgctcatgat gcgggcccat gcaccggcct cgccattggt gtcacgcaga tcccccattc
     2521 gtttattcag gttgttgact tctgtcagga aggctttgta attaccggac atatacgatt
     2581 ttgctgcacc ggaagatgct gtattttcag ccaccttata tccggtcagg gtccacgtgg
     2641 tatgttcctg atcctctttc gtgctgatca caggagacat gttgctgaaa cctatggcct
     2701 gagtactggc tttaaatatc tctttattgc ttccttttgg ggctgttatc aggtcaacgt
     2761 ttaatcctga gttattattt cccactttct gtatatcgac cgacagactg ttattgcttc
     2821 cggttaactg atttttaaca atgagtttat ccgattgatt tacagactgg ctcagatcag
     2881 cccgcataat cacagcagta ttagtggcat ccagtgtgtt tactgtcaca gtatggaaac
     2941 ccgaattctc tatgttacta aacaacaaaa gactgtcgcg ggtttttaat gattgcaggg
     3001 ctgactgtcc gtttactctc cataaggtgt tatacatgct ggctgaggca gcaggagcat
     3061 caatacctcc ggtataagaa gtatcaaagc cacccagcaa acctgtagcc agcggtatgt
     3121 gtgtggtttt gtccaggtta tcttctttat cctggtcaac accaaacaga atagatgctt
     3181 ttttgtctga ttttatatta ccattgaccc aggcatagtt ttgagccttt aacgtggcac
     3241 catcttctgt catattatat ccatctgaca tatagattgc cgggctgtat ttattatttt
     3301 ctgtaggggt gcctgttaat gtcagggttc cggccagttc aatagcttta tctacataca
     3361 tttttccctg ggcggtaaca gtagctcctt tgttgataat caggtctgta ttaattaagg
     3421 ataatgcacc agtcatagta acagttgatt tatcaccaac agttattttg ctgttttgag
     3481 cactcagact gccgttgaaa ctactttcgc ctgctgaatc cccggagtga acctgttgtc
     3541 tgaatccaaa accttcgcca gtaatgtttc ttcctgagaa catgtcaatg tatgcagttt
     3601 tatttcccag aacaatttga gagtttttag cctctatgtg tccttttaca ttagcatccc
     3661 ggcctatact cagggttgcg ttttctaaat ttaatttccg gaaattaaat ttacgacttt
     3721 cccagtcggg ctgggaaaaa gaggctacct gattgttgga cttatattcc gtattgtaac
     3781 gtttatttac agtgctttcc tggtccctta tggcagcaga atagtctttt tgacataaaa
     3841 gaggaattag acagttgtta ctgccttctt taaatatggc atgttctgtc gcatgtcctt
     3901 gtaatgttaa ctgtgcattc cggacagaga tatcgttttt gatatcgact gagccatcaa
     3961 agatcagatt ggtattattg ttatgtttct gggtagtatt gataatatga tttatgctta
     4021 tattacctga tacattccca tggtacatat atgcatcagt attagtaagt gataatgttg
     4081 attgcttcac gtttgagttc gttacggttg ttcccgcatc tgttgctgcg attttctgaa
     4141 atgactggtc atagccgttt agatccagtg ttccgccatt tctggtaaaa tagatttcgc
     4201 cattaccgct ttcgcttaaa gcgtctttgg catttatttt taccgtacct ttacctccgg
     4261 ccatgtaaat tttattgaag gctttttcag cactaaggat gacagtccca ttacctgttt
     4321 taaggttatt tccctgtgct gtttttacat ccagagtacc actacctatt ttatgcagat
     4381 tatctccggc aacgccctta acattccatt caacggtggt attattatca atatctatcc
     4441 cggcaccttt ataagactta tcttttccgg acactttata tgtctggttc ttatcgaata
     4501 taagcccgcc aatgccaaga tcggtatcct gcttgagcgt taattcccct cctcctgaga
     4561 cagacaggtc cttatctttt ataatcgatg ataactcttg ttttttacca tccagctgca
     4621 tagtgtttgt gaaattattt tttaaattgt ttacccggtc attattaaat aacgcgtagt
     4681 ttgtccaggc tatattggta ctactgataa aaccaactgt actggtaaca ccatggaaaa
     4741 cccatttttg ttgttcttta tcatatagat aagaaccaga accactatcg ccaaaaattg
     4801 gtgagttttt aaaaggggtt ctgttggtat aaatgtaact cttgtttttc cagtccagat
     4861 taaaaagact tgcgcttaac aagtcgggat tatagttttg tccaaacaga tattgtttgc
     4921 cgtttaatat cgttgaagtt gttccggcac ctgctctgaa gccaattatt tgttccttac
     4981 cgttgtagtt tacgccatat cttttcatcg catcttcggg agaaagtgag aagtcggcac
     5041 tctccgtaac ccctgttgtt tccacgacga atttattcag acgatgaacc gaaaagtcgc
     5101 cagatgatac tctgttactt gctttatatg ctgtctgatc ccatgactgt gttgttattg
     5161 catgatgctg tgtaccgtta tgagtcgcag taacactgta cgcacctcca attgatgttg
     5221 ttgcaccttt gttggaaaca gcagaaaagt caggtacagg tagttctgga aaatgaaata
     5281 tctttccatc tttcatcatt aattgaacat ttgttgcacc cggtctgaac tctcctctgt
     5341 tttgagcaag gtcaagataa tcgcgggtcc atatattatt tatatccagc attgctgcaa
     5401 atgctgtttc gccataactt ccatatattg aggataaaat aataccgagg ataactttct
     5461 ttcttgatgt accgacacta actttccggg tcagttcaga aacagcaact aatccacctg
     5521 taatatgact atatttcagt gaataaattt tattcatacg tgaactccga tatttctgag
     5581 ttgtggttat gggataacat ttaattacaa cgggtaacta tcagtcacgg cacgtaactg
     5641 ataggggtat aatgataatc actgtcagtt atatttgtat gacagatgta acaaaataaa
     5701 aaaaagataa acacaagtga ttgattgatc agatattgat cacttggtgt ttgtgttttg
     5761 aactctgtga tcaaaatttg atctttgttg tttccggtca atcctttaca ggttttctgt
     5821 attatccggg ggagttaagt ctgtcgatgt ccgacagact tttgatgatg gaaacaacat
     5881 gcagcgataa tgtgcatgct gttttgcagg tggaactcag aaggtgaact ggaactgtgc
     5941 gaccgcaccg taagtgttgt ccgtgccgat caggcctgca acatcaaggt gaaccacatc
     6001 aaacggtgag atccccaccc cggccgtgaa tgcactgccg tcgttaccgg ccagattctg
     6061 acggtaaccg gcgcgcagct gtgcccattt ccaggcatta aattctgcgc caatggcggc
     6121 aaactgacgg ttgctgtcgg aggtgaaacc gctggccggg gtcagatcca catcaaatgc
     6181 ggtggtgaac atcgcgttgt gccaggagac accggccgtc acctgcgggc ggatcctgaa
     6241 ggtttccgtg ataccgttca cttcttttgt ctcaatacta cgggggatca ggttctgtgc
     6301 gaccagcccc agcgtccagt tgtcatccag gtccgtactg gcaccgatgt cggcgttgat
     6361 gccatttttc gtgttgtggt agcggtcgcc tttgaaggcg ctgctgtcat aatttctgac
     6421 cagcacgttg tagttgaaca ggtcgacgcg ctggaatttg ggggtgaagc ccagagacca
     6481 tttctgcccg gccgtttcca gttccgtagc cagagcaata cccacatcag tgatcagtgc
     6541 cgcgcgggca aaggcctgtg atgtcaggcg gcttttgtcc acctcctgtc cggtgctgtt
     6601 tgccacctct tccaggtaag tgaggtcagc atcagagact ttaccgtcga cactgacggt
     6661 gccgtgtgat ttaaccatca gtgcggcaga gaggcggtca ccgggaaggg ccgccacagc
     6721 agaaacgccc aactgtgcgg cggcatgtgt gtgacggaag tcccggaggc gttctttcag
     6781 gcgtgcggca gcctcaggaa caccgtggtt actgtcgatg gcgcggtcga aggccttcca
     6841 gtcatcactg atccggtcgg cgttgtcggt gatattatcc gggtcggaga gttgcgcacc
     6901 gacggacgga agcagaaggc tgaaatcatc gtcaggaccg gcttttgtca gcagcgccgg
     6961 gtttgccagc ggtgccacgc catagtgaga ggaggcgacg ccggttccgc ccattgcatc
     7021 attacgggct tcagaatatg aggtggcggc cgctgaatta aaggaaaata aaacggcaga
     7081 cgcgacaagt gtacgggtaa tatgcttttt catcatcatt tcagtttcct gtattaaggt
     7141 ctttcggata tagcaaaggc gtggcacgcc tgtgccatta aaaacagtca gtgtttaata
     7201 acgtgttatt accagggcga taagggaatt cggaataact cagccggtaa tatttttcct
     7261 gtgaccagat attccgcctg tgccggactg agcgtgacgg ccctgacggg gatatccggt
     7321 gacatttttc tggccatctg aaaccagccc tctttctttt tgttcattcc tgtcggatat
     7381 aaagaaagta ccgggtaaat aatggcattg ttaattttct ttttctggcg cagaagaacg
     7441 ccacaatatt cctgagcgtg ttcaccggaa aaaggaaaac catgccagag aataatatcg
     7501 atattatcgt cttctgaaat aagacggcag aacgcgtcct gtgtttcggg ccagtctccc
     7561 ggatgacaca gcggataatg caccctgccg ttcagccatt caggactgag ataaaaccag
     7621 tcagccagtg aggcaaggat ttccccggtt aggtagtcag tgagtctgtc cggctcccgg
     7681 aagacggcag ggcggatatt ccagggggcc agtaatgtgg ccatggtgac cgggctgagc
     7741 tcatgcacct gcatgagata ttccatacgg tcgtgaagac ggcggacgct gttatccgcg
     7801 gggagaaaca tctcacttaa tgcctcttcg accaggatac gcgtcaggtc agagacggag
     7861 agggaaagct gttcgctcat gatgctgagg gcttccctga cgccctgtgg ataacgaacc
     7921 tgcagtggcg tgggctgcag ctggcggttg ttgagcaggg cttcacaaat ccgggccctg
     7981 tccagggaga gtatcatgcg ggcagcaatg ccagtgctgt ttggtttcat tctgttctcc
     8041 gtgcggctca gcgcgttgtg ttcataagtt gagtctaaag agagtgtttt ttaaacggaa
     8101 atgagttatt tcgatcgtta tgatcgagtg ttttaaaaga gtgttaacca ctctttgtag
     8161 gggcccaggg ctgggtgtgg ccagatatgt tgttggtctg cattctgcgg cagaagtggt
     8221 tatttgtatt gcgtggggag tattgcaggc gcccttgttt ataaggtcat aaggttgttt
     8281 ccagaactgt tgtttattac atgaagtcgt ataatattaa aaaagtgaat ccggcagtat
     8341 ataacactaa cagaataatt tgctttggca gtgatagttt cttaaatctg tcactgtcat
     8401 atgccagcca gaatgcagta cagatggcca ggcctgataa gaatatggat ataagtaata
     8461 taatggggag tggaatatgt ccggataccg gaatattatg ccacaaatat atctgtatca
     8521 ggacctgaga cagaatgact gtgattaagt taatccggaa tttatagttt attttcattc
     8581 tgatatgcct tcagcttgtt aacggacaaa tcatgacccg tgcatcaatg tttgcgggta
     8641 gctcgcctgg cgttggaacg cgttcacccc gctgagccgc ctgtgccagt gtgcgcagca
     8701 acaatacgct ggcatgagat attgccagtt cccgggtttc agcacgggtt tcaaggtcga
     8761 aatcgtgcag tgtcacaatg aacagtccgt ttctgttccc gactgttgcg ggatagggaa
     8821 caaaataggg ggcgattttg caggtctgtt tacgctttgc atcgagcttg ctgaccagct
     8881 gatacgcccg cagatgagta tatcgcttca gcatgttgag agagcgatgg ccggaaatgg
     8941 ccgcaacctc catgacgttg agtgtgccga gttcaaaaaa acggctgatg gcttcatggc
     9001 gaaggtcgtg aaaatgcaga ttttctattt tcaggtccag aagtgcggta cgccaggcgc
     9061 ttttgaaacc ggatgaggtg taactgaaca cattgccgtt tatctgttgt ggcagaattt
     9121 gcaggtagtt gcgagctttc cgggagagtg ggacatcgcg tggtaaacca tttttcgttt
     9181 caggaagatg cgccactccg tgctgcaaat cgaggtgttc ccagcgtaat gtgaggattt
     9241 ccccctgtcg cattgctgtt tccagggcca gatggaagat aacgtagagc tgctgatttt
     9301 tatctctgaa atagcgggag agtcggcgtt cttcgccaga agtcaggcga cgatcgcgtc
     9361 cactactgat tttgggtttt cggaccagtt caacaggatt catacggcat gtcccccatt
     9421 ccacactggc gatattgaat aatgatgata aaagagcaag ttcaagacgg acggtgtttc
     9481 cggtgatttg acgccctgtt cgtgggttaa tttgagccaa acgctgatcc cggtaagtgg
     9541 cgatatcaac ggtggtgatt tcatccatat agcgctcggc catagggtga cgtttaatca
     9601 cattgacgcg ataaaactcc tgcagatgac cttttttgtg gatggatact gtcttcaggt
     9661 atttgtccag cgcccggttg agagtcattt tgcgaatttt tatttttcga aacatatctg
     9721 ccttccacga taaatcccag gaaggtagga atactgaatc agaaatgcaa tgtctctgtg
     9781 ccaatccggt atctggacaa cgacaaaaat gaacttcact accagtacat acaatatcgg
     9841 gaaaaatact cgtaatctga gcattggcgt acatgcgtac tgctcgtgga cataccttaa
     9901 tggctcgcct tttggtggtt ttcagcagat atacgccgac aaaaacaagg tttggtatgt
     9961 gaataattat gcgtggggaa attatgagtc cggtggcaca attacagtga catgcctcaa
    10021 ccttcctggt gccggaatct aaccaaaagg gcaacagtaa gcatcgacgt gctcttcatc
    10081 aacaccatct ccgatgccgt acattcttgt acctgtcatg acgtacccgg cagggcactg
    10141 aatcggcttg taataaatag aacctccgct tctgccaccg ataaaatgtg cggaggtcgc
    10201 ttcataccat ttacaggcag aatagttcac tttattgcct ccaagccaca aaccggatta
    10261 gcactggcct ccgtgccaaa tccggtgtgt ggcgaacgat tggtgcacct gacggaagtt
    10321 acagcaatct gggttctcac cggggaacat ttaatgggag gaataacggc agaggaactt
    10381 tgtttgtgta cgcttccggt ggaaatggtg ccggtggcgg tgattgcgcc aacacatcaa
    10441 atcttcaggt ctatgtgaac ggcactttca ttggtatgaa tgccagcaat aacccctcct
    10501 acggaaagac agcatttatt tcatttgccg tgccagtggg agcgtcatat caaatcatct
    10561 ccagaccaac acaaaattac gcctgtggta acggtgtttt ctctgtgtat gcctatcaga
    10621 tgtgagtccc tagctggctt taacaatgta gttgaatgca atatttttgg gacgcatttc
    10681 tgtggtactc ccctgtgccc agtatggcat accactatga tgccctttca gccatccgcc
    10741 acgttcgtga tagacgcctg attgatcggc ctgataactc aggagttgtc tgttccgatc
    10801 accatcaagt cccttcccgt tatcccagcc acggataaaa acaccgcgca gatcgggcaa
    10861 tttacagcca ggataagcac gggcaagctg aggataccgg gaacaggaaa acgactggcc
    10921 gttcatcaac agatagcctt gtggaggagt ggcagatggc cacgggatag gacttcccac
    10981 ggggtaggat tcaaaccccg accaggcacc ggattggcac tgaagtatgg ccccgctgtt
    11041 gtcgcggcct acaagcccgt ctggtgagca atgagtgcct gcgttggcga ttccgtttag
    11101 ctgtaaatac tcgccggcag taagacgacc atcagagcgg accgttcctc cagtgactgt
    11161 ctggccacgg acagttccac ctttgatctc tccttcggtt gtgatccctt tgttgttaac
    11221 agcacgtatc cactggctgt ccgtcatggt gaagccgcca ccatttggat gtaaccaacg
    11281 atttgatgtg acatcgcctg aaaatctggc gtttttacca ctggcttcat tgatattggt
    11341 gatgctgtta ttattcatat caatgccagt atgcattctg tttaaatcgg gtcttcccgg
    11401 aacgctgtag cggtaaagcc gatcgctttc ctccagatct tttccagcca ttcgcatggc
    11461 gatatggccc ttgttgcagg taatctgata attacttccc agatcggtcc agccgccaaa
    11521 ggcacctgta acggaattat tctggagcat aaaaccaccg agtccattaa tctggcccgc
    11581 tatgcgacgt aacccatctt ctttaatatc ctgtcctccg gttgtgcagg taagtgcttc
    11641 cagacgaccg gataaattat tttttctgat accggtaata tagctgtgtc caaaggggga
    11701 gcgggaaaat ccgttgttca gatatccctg ctgaattaat tcatttaccg tgatggtttt
    11761 actggaaagt gagctggttt tatcagccac ataatttttt attgcattat tatattcatc
    11821 agcatgtaaa gccgcacttt gataaagttg ttcattcagc cagtcttcaa tttctgaata
    11881 aatcattggc gtggcgatac caattagtat caggccagca agaatttcaa gcagaataaa
    11941 acctttatcg tcatcgagtc tgaatttttt cacgtgaata tctccgatgt gtggataacg
    12001 gaatcaggga agagggtcaa cacatacagg gacgcgcaga gaaacggagc aaatggcagt
    12061 gcctgctggc gtctgcgacg gcagaaaatg aggataaaca gaaatgcccc ggaggctgcg
    12121 gcggtgatta tcagtggagt gtgttgtgct tgtgtccaca ttgcgatccc cgcaatcagg
    12181 aacacatcac caaggccgag cgcttccgtc cctttcactg ccagaaaaat gcgtcgtaac
    12241 aggtatatca tgccgaacca cagtgacgcg ttgagcaggg catgagccag tgagtcctgg
    12301 ctgtatctga acgctgcgcc cagaataata agagaaagcg tgaatatccg gggaagccga
    12361 cagacagcaa tatccgtcag cgtcattaca gttaataatc ccgtcagaat ggaaaggaat
    12421 aacagtcctg tcgcgtcaac accatatgca ccgttgaggc aaatgctcag tacagccaga
    12481 agacaacaga tgcacaccgc agcgttgtgt gggcgagtat tgtgtacctg cgacaaatat
    12541 accgtggcgg cgctgtatgc aagatacaga attgccgcaa ggacaatcag gataatgcat
    12601 atcgggagta attcagacac agacagtcac ctcagcttaa tcttttctga aataccggtt
    12661 gtggattatt cgggcatata tcatgcgccg ggctgtgtta tttttgctga atcctgcgtt
    12721 gtaactgcca aggcattccc agttaacgcc gcagcgttga aaatggtggg ccagtatcca
    12781 ggcgccggta tgaatattca ggcagggatt tttaagcagt tcatccttgt ctttaatgac
    12841 gcccagtttt ttcagttcag gaatatgcga tgaattaatc tgcattattc cgtaatccgt
    12901 actgatgatt tttccggatt tatttttatt gtgatttatc gcagtggcga ccaggccgct
    12961 ttcctgctgt gcaatggttt tcagaagctc aggggcaatc aggtaacgtt ttccggcctg
    13021 aataaaacag gaatcgagat gattattatt catcacagat gcgattgatg tatgactgaa
    13081 taaaatcagg acagagaaat ataaccattt cattatgata atcccggcag tgacagcatc
    13141 ctcttgtatt taaggatgct gtccggaaac attagctgtt tgtttcaagg gtgatgttat
    13201 ttttgtcctt actacatact gttacaggat ccacagtagc cggggtggta ttcatgattt
    13261 tggtaaacat ggatgaactg cgcaggctgt taatgagttc cacgcaattt tctttcggta
    13321 cattattgct ggtaatcgta taaccatact tatctgttgt tgccttaatg gtgacatcac
    13381 cgccccaggc atttttggcc ttgttaccac cggcgatcat atcggccggc agggaaccgc
    13441 tttccgtcag ggttttgata taatctgagc ccgtataccg gccctggaat ttatactgct
    13501 tcattttggt ggcaatggtc tgcaggtttt tcagttcgcg ctggttatca ctggatgaca
    13561 tggtactgga catctgactc attgcgagac caaccacaat accgataata atcagtgcgc
    13621 cggcaatttc cagtaaggtt aaacctttat cctcctcttt ctcttttttt aaagaaaaat
    13681 aataaaaaag acgtgctctg aagttcatat atcactcctg gtttatggga tgtttcagaa
    13741 atattctgta tggggaaatt attgcttcag catgtcgctg atactgtaga tggcagatat
    13801 catgttaaga ataaaaaaga agacaaaacc tcctgatata ccggtaataa ttaatccgct
    13861 gcgttttacc cgtttttttg cctcttccag ccactggtcg gcatagaccg ccatggctgc
    13921 cacactgtcc tccctttcgg aattcaggag cagtttattg atgcatactc tggacggaaa
    13981 atcgtaaccg gaatttcgca ttgccgtggc cagattctga ccggataaca catgttttcg
    14041 tattgcctga attctctcct ttaaccattt attcttttct gccccttttt ccattctgag
    14101 cagtgcctgc gaaagcggaa tattaatctg cagcatggat gaaatactga acagataatt
    14161 cactccgtgt aaggtctgat acagtgacca gggcggtacc ctgtccagca tcctccggcc
    14221 ggggccggtg tacctgctga aggaaaaacg gatcagaacc gtcagggcga taatggcccc
    14281 gcagagccag agtccgtacc cgatgataaa ttccgacatg ccggccagca ctttcatctg
    14341 gaatgaccat ttatcttccg tggcaaacgc actgacgatg ggaatgatac gggtatgggc
    14401 gttgtacatg ttcaccacac agagtgaaag cagcagaagg ggatagaggc agacggcaat
    14461 gaggctggat gtcatttccc gccgggcttt aacggttctg gccacctgga aaagggcgtc
    14521 ggcaatattc ccgcgctctt caccgtttct gattaacgtg gcttcctccg gtggcaccca
    14581 ggtgggcaga gcaccggata acatgccgct ggatttgatg gacgcgatgc attccttcgc
    14641 cagaaaatac atgcctgatg tccctttccg ggttccgaag tcagtttcga cattgcagag
    14701 aaattcgagt gcacgcagtg acggggtccc gtcacgtatc atgccgccca gggattcgta
    14761 gaactccacc cggttcaggg caaaggtttt tctggctatc cagacggtca gtgctgtcag
    14821 ctcaatgtcg gtgttgttga gtttttccag aaatctcatg tcagtgcagc agcgtatagc
    14881 tgtcctcgtc gatgggagag atgtaatggg ctgccagcgg atcaacctgt ccgctgttga
    14941 tttttcccag gagatgctga tttcgggtca ttccgcccag ttctctgtgc cagtaggttt
    15001 tagcctcaat acgacccttt tcccggtaca actggaaaaa acgcgcatcc ggggagatga
    15061 cttctgcgat aaccgttcgc cccgtcacgc cacgccagca gtgtgagcag ccttcatgat
    15121 ttcgcagata cacctggtcc gggttacaga agccttccac caggcgccgt tcatcatccg
    15181 tgcgtccggt ggccacctca tgccaggggc gacggcagtg cggacatatc agctgaacca
    15241 gacgctggct cagcaggccg atgaacaact gtgggtcggc gatcatcctg gtctgaacgc
    15301 cttccatctc aaggcgttcg aggatattaa tcgggtcgtt ggcatggatg gtggtcagca
    15361 tcagatgccc ggtcatggcg gcttttatcg ccgtaatagc ggaatcgtgg tcgcggattt
    15421 caccgttcag gatcccgtca ggatcaagac gcaacgctga tttgatggag tccacccagc
    15481 cctgcgcggt atccatgacg gcggtctgaa tggcaccggg aatgcgccct tccggcgggg
    15541 attcaatggt gaacagcctg cgacggggaa gaagaatacc gccggtgttg ttgaagccgt
    15601 actgttcaag gtacgccgca gaggcggttc tcagcgtggt ggattttcct gagcctgtgg
    15661 ggcctgatag cgtgataata ccttcagggc gctgcagaat gcggcgaaga gtgcgctcct
    15721 ggtcagggtg atagcccagc atactgaagg tcgggatttg ctggctgtca tccttgatca
    15781 ggcggagtac ggcatagaga ccacccacgc aggggtagtg ggaatagcgt gcaccaaaca
    15841 gaccggccag tttcaggtac tgttctgaaa gacgcgcatc ctgaggcacc ccgggatcaa
    15901 actgtgtgcc cttgatgacg tcggtcatgc cggaataggt ggcccccagt aattcgaggc
    15961 cttcttcttt ccggaggata tcgagcactt ccagttcgcc gtgaacccgg gcttccacat
    16021 aggtgaagtc cgtgttgtcg cgtcctggtg tgatatgcag atctgaggca tccagttgag
    16081 cggcttttct gatgtaatca agaacttttc tttgtactgt gttgatccct ccgtcggata
    16141 aaacgcccgg tgcacgttcc gcattattgc tgttcagggc tgcgataacg tcaagagtgg
    16201 taaaatcgaa tttgctgatt tttcccggat agcgggagcg taagagcgtc aggtaacgtt
    16261 gtacagaggt gatgtgcttc ttcgcctcga cgactttcac cctgattttt ccgtcagcgt
    16321 catgctgaat tgcgacgaaa tcacgaatat cttcaggagg cagtacgata gtctctggca
    16381 tgatggcacc tcacgggcgt agcgtctgcc tgttgccgtt gtattccaga atcacactgt
    16441 ccggtgtgat ttgttccacc atgaacagtg taccgggtaa tgcctgtccg gccctgacat
    16501 cagtttcagt tcctccgggg agtatgacgc gtgctgaaag ctgtgggtga ataccaaaga
    16561 tttctttcag acggggggat gataatgcct gtgaaccgga ggaggtgcca gccgcatttt
    16621 ccggctgatg tcccgtgaca gaaccggaaa ccggggagat accctgttcc agtgtccgca
    16681 attcatgacg aattttttct cttgccagca tcgcttcata cagggcggtt tcttcctgaa
    16741 tctgctcaat ctgcagttgt ttgttctgtg acgcgtcatt ttcgttgccg gccactgcca
    16801 ttgccggtaa cagcagggct gcaatcagca gcgcttttgt tttatcgtgc ataatattta
    16861 cctgtgaact gataacggaa acgaccattt tccagttgtc ttgtgacgga gagaaaacgg
    16921 acagatggct cgctgaagcg ctctgccagc tggttagccg ggagtagcgt ctgtatctgc
    16981 aggtcatact ctttccacgg tggctggatg atgtttccgt tttcatcagt gaagctgtta
    17041 ttgacttcct gccagtcaat ctgcagttga actgactgtg cgagtgtggt cagtcgttcc
    17101 tgcaggtcac tggtttccgg aagggaatca accgggacag gattatccgg gagccggaat
    17161 gtgaccggca gtgcaacacc gccagagttc ccttcaggca tcacaataaa cggccgggtg
    17221 tctgtgagtt cagtgatgcg ccggatgaag gctgtcactg tcgtgcctgc tgtattggtg
    17281 tatgaagccc gcagattacc gctgttaccg tcagtagaac attcagccag ggtataacgc
    17341 catccggcag cagacagtgg cgtctggttc catgttgtgc tgcattgccg gataaattct
    17401 ccggtctcag gctgccccgt ccatggtggc gttatacgtg cttcagctgc tttccgggca
    17461 ctttcttccc gttccgcttt ttccgcctgc cagatttcat aacgttgcca gccgtaccac
    17521 gatgcgccaa gcagcgtgat gatgagtaag gctgtcatta actgtgggcc tctggatacg
    17581 gggagcagac gtgcttctgc cggatgcttt ctggtgctga tcaggtttga cagcggtagc
    17641 gggatacacc tgctgtctgg tgaaaaaccg tcaggttcgt agcgtaccca gccgttttcc
    17701 ggttccgcat tgagcatcag gaacgtgttg cgggcttccg tgatctcagt cggggtaccg
    17761 gtaacgtcgt taaccagaat gccattaatc accgatacaa acgcacaggt gagttcagcg
    17821 tcgtcgttct cgtcgttccc gatgatgaaa atggcatacc cacctgattt gagtagcggg
    17881 cgtattaaca ttgccagtga caggcactgg cgaatgttac gggggatatc tgcatcctga
    17941 cgactgagca gggcaatcat catttcatca ccgttttcac cgtcgtggtt gtggcaggaa
    18001 agatacagtt ctgcgccctg tgaccgggca aaccgccggg cattccggta accgggaggt
    18061 atgttcttcc agtcaaggcc cgcaaggaat gcctgtttac ccgatatcag tgaaaccgga
    18121 cgaatttttt tctgcattga cccgttcaag ggaacctccg tcagattacg tgcggtgtga
    18181 tcagcacaac cagaatggtg tcgtcatccg cactgtccac gccaccaccg agtccgaaga
    18241 atgacggggt gcccacgccc tgtctgagcg atttccggct gaactgctgg aatccgctca
    18301 ggatcaccgt ctgatctgat ttcaggtcga tggtctggtt gatggttttc agctcaacac
    18361 tcggtgtctg ggcccgactg ccaccgcttt caaacacttc aaaaacaggt ttgtcggaca
    18421 ggctcatgct gaacagtaac tgcaaccggt gtgagccggg ctggataaac ggtaacagtg
    18481 tcatgttgaa gccggtggtg attgttgccg cattcagtga ggtggaggag cccacattgg
    18541 ccgtcgtatc cgtggtgaca ctttctatgt acccctgctg gctggcgacc tgcataggaa
    18601 ccggcgtcag gtttttggtg acagccgagt tttgggtgac cacactgact ttaccctgtg
    18661 atgaaagcgc tttcagaaag gcttttgaac cggtgagctt accgtcaata atcgataccc
    18721 cactggtcac gacttcatcg gcggcattag taaatgcact ggtcaggtcg ataccgagat
    18781 gtccgtcact gaaaacggcg ttccagtcga ttccggcctg ctctttgctg gtttttttga
    18841 tgctcagaat ttcaatattc agcacaacct gacggcgcat ttcccggtta cgttcttcca
    18901 taaaattctt aacagtatcc agaacctgtg gggtgtcggt tacggtcaga aagccggtgg
    18961 acaacaccat ccttccgata ccgggcgtca gcatggaatt cacggcattt tttgtgtctt
    19021 catagagtgt ggatttcata tcaacggcgg ttgtctgcgt attgctggca tccccactca
    19081 gggaactgcc ggatgaacca ccggaactgc ccgtggatga catggtgcca ctgacgactc
    19141 tggcattgta ggcgacctgg ctgtccatgt aggtgaccgg gaatgtacgg gtgtcaagcc
    19201 agtggaattc aatttttcct ttcacatagc gccaggagat cccaaagcgt gaagatacgg
    19261 tgttaagcaa agcgggcagc ccctgaacat tgagttccgg cagtgaggcc gtggaggggg
    19321 cggctgatga cagccccacg gtgctcagtg gctgcatgcc actggtgtca ggcgggggaa
    19381 tgtcgccgct gatcctctgt gtgcgatttt ctcctgtacc cagcagtgtc cagacatcag
    19441 acatgatttg aaccggaata tgacagcttt cagaaatccg ctgtgcaatc tggtgaagtg
    19501 tgatggcttc ccgggtgttt atgacgaccg ggcaatctgg tattgcccgg gtaacggttt
    19561 tttccggaag gggcgtggta ttgatccact gctggtttgt ttcgcgcaca acggcgcgtt
    19621 gtttgcgaag ttcaccggct atacgttcag cccgtgttat ctcatcggat gcattttttt
    19681 ctgtggctct gattcgttca aaactgctgc agcctgataa aacaacggat aaggcggtga
    19741 cagcgcagaa aaattttttc atattctgta tacctgcttg ttgaatattt attctctgtc
    19801 tgtggaggga cgaaataaca attcagtgag acagagcgac gggtatttca tgtgttaatg
    19861 aacactgaga aagattttcc ggactgaagt ctgcgccgta gcgatatatc atgtcgctga
    19921 gagaagacat gatatgcaga cctcagtaca gaatcccctt ttgggctgat tagcgctgat
    19981 ttatggggag ccctcagcct tttggggatg cttaattgat caggacgatc gcattttccg
    20041 gtataacgga aggcggaaca ccaatattat ttccctggtt atccagtaac tttctgtccc
    20101 tgacctgcgc aattaatgct gagtttcggc tcactttatt cagggctgat atcagccctg
    20161 gctgaaaagg aagccagaca aaagttcttt gccctgaaat aatatgtttt gttccgtata
    20221 ccggagcgaa tccggtttcg ttaaccgtaa gcatctttcc ttcacggata tcaccgggat
    20281 gatgatattt ccagtcatta atggcatttg tcaggcgtaa tatttcatat acctgttgtt
    20341 gtctttgctg ctcatcagta ttttttattg cccgctccgt attgccggca tgaaaccagt
    20401 tcccgatgat aaacagtgcg attatgaggg gcatgagcca tcccatttag ctgtctcccg
    20461 ggatgtcagt aattatcagc acacactggt ttttattcac cgaggcatat aagggttttt
    20521 cagcctgctg ataaagtgcg aatgtactgt tcagtgcatc cataaaatta cctgaaaaaa
    20581 ctaaccgggc atcgatactg taatttaccg gcgttttcca tgccactttc cattttccgg
    20641 tagcgcaggg ttcggcggct gcccattttt ccagccctgt acgcagggtc attccctgct
    20701 caatatgacg tgtttctgtt tttttgacac cggtgacggg ggctgagaac aggccagatt
    20761 ttaccgtttt ttccgcctgc tggatcagta cgcgttttcc tgaggtatca aatgtggctt
    20821 tcaggctgta gcggggcagc gttttttcga taacatggtt ccactggtca tttccggacc
    20881 atgatattct ggcggggagc cggacgtcac gggcaatctc agtacgccag cctgtgggta
    20941 ataattgttg catcgcctga ccaaacggtc ggtgtccgtc ggcccgtata aatttcagcg
    21001 ttccgattgc acgtttttcg gcgtggttgt tcgatgatga tgtctgagtg gaggctgtgg
    21061 cggtatatga aaataagaac gcgaagatcg aaatacttaa gtatcttaat atattcatca
    21121 gtatcacctt tttcactatt tctgtctgtt aagacggagt tgactgaatt aataaaacgt
    21181 ggcgtaaata aaatgggcac atgaattgat taaaataaga tgaagaaaag ccagggttat
    21241 ataaataaag gtgtttttgt ctctgtgtat gttgcagaga taacaaatcc atgcgtgagt
    21301 taataacgaa ttacagaacg ccagggaaat cagcgagttt gtaagcgcat tgtccggtgt
    21361 attaccgaaa acaggcgtgc tgtctgaaat actggtgcct gtcaccagga atgcccagaa
    21421 tattgatgaa aggaaatatg ctgaaaaaag agacaaaaaa tcctttctgt atctgttgta
    21481 aaagcccctt acagtcatat ctgcggggat ggtcgtggcg gtggctatta taaaagtgat
    21541 aataaaaata acgataacga aaaacagaac caccggaaga aaatctgtat atgtgatggg
    21601 atgaggcata ataaaaatca ctctccaggg gcaggtggat tttccttaca tgaaaattga
    21661 gtaaccccga ctttaacggt gaatgattta ccggcttctt tttcagtgga gaagagtttt
    21721 tcacactcct gtttcgtcat gggcgtattc tggaagcgaa tccatgaaac cggtgtaccg
    21781 ccgggttttt cgatggtggc ctgaatacag tatttatacg ggaacggggt ggcctgcgct
    21841 gatgccatta tctgtactcc tgctgtgcat tatcattctg tgctgaaaaa aggcgacatc
    21901 accatgggga aaatgatgtc gccgaaagtg cctggtttta ctggctgttg ttcaggactc
    21961 tgcagggtgc aggctccggc acaaccggaa acgtactgtt ctggcgctca gtgcgttttc
    22021 gggttgtgta gccattattt gcggtgtccg gcgttgggag tgttacctgt ggctgcaggc
    22081 gcggcacgtc actctctacg tagcatatct gttttcctcc tcagcccggc ttcagggcgt
    22141 ggccgctccc ggattgttaa agagcatgac cgttacgaag aacggtgata cagcaggccc
    22201 gttgtgggct gtgtctgagg ggataatgaa cagcggatac ccgggtggcg ataattaatt
    22261 cttttcaggt cgctaaaaat ttacgggcgg taaaattttc tggtcaccag ggtaaattaa
    22321 tgctggtcac ggtcgcctgg ctgaaggcag agtgaccgga aatttcgtct ttacggagga
    22381 aagccatgcc ccataaaccg acagaagaac agacggcaat tattcactgg caggggagga
    22441 agcttgttgt taatgctttt gccggtacag ggaaaaccag cacactggtc agctatgcca
    22501 tggcctgccc ggagagcagg atgctctatc tcgcctataa tcgtgcgatt cgtgatgaag
    22561 ctgagcgcag gtttcctttt aacgttgaat gcaaaacgtc gcatcagctg gcgtttgccc
    22621 gcttcggaag gcattaccag aagcggcttg ttccgggctt gttcgtcacc gatgtggcaa
    22681 gaaaactgaa tacccgatac tgggcactgg cccgggtggc catgacaggg ctgaatcagt
    22741 ttatcggctc agcggatgca acccccggca ggcagcatct gccggacaag gacgaaatga
    22801 aaggcgttgc tccggatgag gcacttcgtg ctgtgaggac gctctggcag gccatgagcg
    22861 atccggatgg aacgtttccg gtcacccatg atacctatct caaattgttt cagctctccg
    22921 gacctgatct ctcgcgcaga tgggacacca ttctgtttga tgaagcgcag gatgcgaatc
    22981 cggtgacttc cgctcttgta ctgggacagc ggtgcaggat tgtgctggtc ggtgaccggt
    23041 atcagcagat ctaccgtttt cgtggcgcgg atgatgccct gtctcatccg tctctggccg
    23101 gtgccgaccg tctctggatg acacagagtt ttcgttttgg ccccgcagtg gccgcgatgg
    23161 ctaatctgct gctgaaacgc gcaggggaaa gttgggaggt gagcggctgc ggcggtgatg
    23221 atgaggtgct taccatgcag gaaatgcgtg agcgcattaa gcatggaaaa tttgggggac
    23281 accgggcggt actgaaccgg accgtcgccg gagtgattgg cacggcgctg atggcggcaa
    23341 tggcgggcag gaaggtttac tgggtcgggg gcattgaggg ataccgaacg gaggcgcttg
    23401 aagatcttta ctggttccag atggatatgc cggcccggat gcactctgac cggttgcgca
    23461 gggattaccg gaattttgat gaataccgtc atattgcgaa ggtgacgaag gatgtggaaa
    23521 tgaatcagtc catacgactg ctggagctgt gttttccgtt accaaaaaaa ctggagctac
    23581 tcagggagca cacagtcacg aacgagcggg aggctgatat cacggtttca acggcgcacc
    23641 gcagtaaggg gctggaatgg gagcgggttg tactggggga tgattttcag gatatcgctg
    23701 atccgctgat gtcagaacag gagcgccggg atgaaactaa cctgttgtat gtggcggtga
    23761 cccgggcacg caggacgctg gtactcaatg agctcatgca gcaactggcg gaggaaaaga
    23821 gcaggacgaa ggagaagacg acgtccccgc atgagcgtgg cacagagaag gcggtggagg
    23881 gaaaggatgt ttaagttacg gaagtttttt acctccggtt cagcagtgga gatacccgat
    23941 gacgggcggt cagtgccggg aacaccggaa ggcttttatg ctccgctgac gacagattca
    24001 ctgctgatgc cgaagaacag ggcgcagtgt ctgaagcaga tacgggataa caatatgatg
    24061 ccaaagccat tgtgtgaggc gatgtgttgt gctcccgtat acagcctgct tctctccacg
    24121 cagaatgtcc cggcggcccg ggaggggcgc tggtcacatg cagggggatt cggggacctg
    24181 acgttgttgc ataccagtta cgcggtccgt ctggcccgtg cacatatgtt tccgccgggc
    24241 gcctctgcgg aagagcaggc cgcacagggg gcggcctggc atgcagtgat tttctggtca
    24301 gccctgtttt atcacctgcc tctgcttgcg catctggaag gcgaattgtt cagcggccgg
    24361 ggatggcagc cgggaatatc cgttcctgat gaggcgtatc gtttccggtt caggcagctc
    24421 ccgccacagg gtacggaagc tcagcaactg gcggcagtga ttgccggagg tctgttacct
    24481 gagggggctg cagcctggct tgcgacggtg ccgggagcgc ttcagaatct ggccggtgcg
    24541 ttctggcatc agcatccgga aatggcactc atccgtgatg tactgcagga gtcggcccgt
    24601 caggctgaaa gtccgttgtt aaacggtgga attgttgcca cagccagtca tattgcttca
    24661 gcagaggtcg ttacggggga ggtggttgta tccggtaaaa cggagagcac cgtggcaccg
    24721 gctgtgtccg gggaagaggc tgcggctgaa gggaataccg tgtccggcaa tgctgcagag
    24781 tctgttcagg aacaaccaga gatgcggcaa caacaggctg agaaaccgac gacaccagac
    24841 agcagtgaag gttcagagga cgatgcagca tgtgaaatgg agagtatacc gacagatgaa
    24901 gatacagcta tgctggtcag catgtttgcg gaggaggcga gggcgccgga aaagggggag
    24961 gatgactcgc catctggcga ggtggttgcg cctgtttcat cgacaggaga gatatcagca
    25021 gccggagacg atgaagagga ttttatcgcc tggctgaaga tggggttgtc atcaggaatg
    25081 attgaagcca acacgccttc tgcgagagtg cattatattg ccggatatgt ttttttgagt
    25141 gtgcctgaca ttttttttct gtaccggaaa ttgacgggaa gccagcgcga acgcaaaaat
    25201 attcaggcgt tgtttgagcg tgcctgtatc cacaaaatac gtgaaggaga acgttttttt
    25261 agcgtgcgtt tgtattcggt accagaaaag acggggaatt ataaaaccgt cagcggatat
    25321 ctggttaagg gtcgttattt atatgggtat aacaaagtac cagaggacag tgcatttatc
    25381 atatttccct gaaatcactg tgctggtcat gagttcagcg agatgtcctg atggtgagcg
    25441 acgaaaaaat gagctgggag gaattacttg aggagtattt ccttattaaa aatcttaagc
    25501 cggacactga gcatagttac cgcaaggttg tccgtttgtt taatgagttt atgggaggga
    25561 attcctggcc cggagatgtg acagccagag atattcagag atggcggcgc catatgctga
    25621 aagttcaggg gcgttcgaca aatacctgga ataatagggt gtcacatctc agaccaattc
    25681 tggcgttagg tatcaggaaa aagtggttgc cgcaaacgga gaatccactg gatgatgctt
    25741 ccgttcccgg ggagtccaaa aagaaaaaaa cgcttactga gaagcagatg attaaggttt
    25801 atcttaccgt tcagaatttt gctgaagcgg aggttaagcg tcaggttgtt tgtgacagga
    25861 tgcgtaatgc catgtacccg gcctggtact ggcttacagt actggatgtt ttgcgtttta
    25921 cgggaatgcg ttttaatcaa ctgaaacata ttcgactgaa agatgtccat gaggatgaag
    25981 cagtgattgc actgcaactg gaagggagta aaacgaggcg tgaatggagc gttccgattg
    26041 tgacgccgtt gcagatttcg ctgaaacatc tcctggcgtg tgcgcgagcc aacggtgcga
    26101 tggatgatga ttaccttttt gatgtctgtc tgtacagcga gcactatggt cccgggaaat
    26161 ataagtattg tgccagacgg gcccatcagt ctgtcagatc atttttccgc aggttgagca
    26221 gggaatgtgg ttttaatgta tccccccaca gattcaggca cacactggcg acaaaactga
    26281 tggaggcacc ggagcgcaat ctgcatatgg tgaaatccat tctgggacac aggagtgttg
    26341 ccaccacgat ggagtatgtt gatctttcac tgaaggtggc agcccagacg ctggaagctg
    26401 aactttcgtt atccacggac gagggaacga agagacgtat tcatgaggcc agaaaacact
    26461 cttatggtta cagtgaagtc gagcagagtt cagatgtttc tgaagtggag tcgacagaca
    26521 cagaataaag acatcttgac atcaggctgg aatggtgtaa gcatttttag cggaaacgag
    26581 aacagagcac ctgaggggac aggtgctctg tttgaggaac cgatactgaa accatagtgt
    26641 aaccacactt acacggagat ttcgttatga ccggaccccg tattaaacgc ccgttaagga
    26701 tctcatccct taaccagcgt actttgatgc gtttggtgcc cggaggcgga atcgaaccac
    26761 cgacacgggg attttcaatc ccctgctcta ccgactgagc tatccgggca acggggcgca
    26821 ttaaacctga ttcgcctcgt ctcgtcaatc aaatttcttc aatttactgc agactgcaca
    26881 atctatcatc actttgccgc tattgcacgc attgtcaggc aaaccaggcg gcggaaagcg
    26941 gcattgccag aaggagtgct ggaagcatgt tgaccaccgg gaacatttta atgccacaga
    27001 tgcgtaatcc ggttgccagc agcaataaac cgcctacggc gctgaagtct gccatcatcg
    27061 acggtgtggt cagcggtaat atcagcgcgg cagcccacgc cagcgttaac tggatgatca
    27121 gtaatgggat actaattacc gacaccgcaa tacccagcga gcaggcgaag atcatcgccg
    27181 taaagaaatc aagaaatgac ttggcgatta aaatactcgg atcgccggtc atcccttcgt
    27241 tcatcgcccc gaagatcccg gtgccgctgg cgcaaaacag gacaataatc gcgacataat
    27301 tctgaataaa agattcatgc gctggcttct tacgtgagtg acgaaacaga ttttgtgctt
    27361 tggcgactgc tgtattgaca cctttctcca gcaaacaaat ttcgccaatt agagcgccaa
    27421 gtagcgtagc taaaaccatc gctggaaggt tggcgcattt cacaaccagt aaaataccaa
    27481 tccccagcga tgccagacca aaaatagatg tcatggagac gcggatacgt tccggtaagc
    27541 gttggctgag cagtgcgccg agaacgccac ccagtaagac tgcactagcg ttgataaaag
    27601 ggccgatgac cacaagagtt cctgttagct gtattgtttg attttcttat tatgaacttt
    27661 ttgccttgtt gatggcttgt ctggtcgcaa agtcctggct tgcacgcttt agcgaaaggt
    27721 gccatgattg cgcgaatttt ctcctctctg tacggagttt gcccgatgca cgccatctcc
    27781 ttacattctc tcgcttatcg ccatttcgcg cgaaacgttt ccctttctat gttactgctc
    27841 atgcggtgaa gttaacgcac gctcactgca ggacaacagt aaaatcagag cgtttctgct
    27901 tttactgatg tctggcggtc ggagctggtg accagtttga cccatatctc atggggcagg
    27961 gttttccacc ttgtccggta ttcttacttc cccgaaacgg gtttgcgctt atgaaatcaa
    28021 tgaatattgc cgccagtagt gaactggtat cccgactttc ttctcatcgt cgcgtggtgg
    28081 cgttgggaga tactgatttt acggacgtcg cggcagtcgt cattaccgct gcggatagtc
    28141 gcagtggcat tcttgcgttg cttaagcgca ccggttttca tctaccggtg tttttgtatt
    28201 ccgaacatgc tgttgaatta cctgcgggcg ttacggcggt aatcaacggc aacgagcagc
    28261 agtggctgga gctggaatcc gcagcctgtc agtatgaaga gaatttgctg ccaccgtttt
    28321 atgacacgct gacgcagtac gttgagatgg gcaacagcac ctttgcttgc cctggacatc
    28381 aacatggtgc gttttttaaa aagcatcctg ccggacgcca tttttacgat ttctttggtg
    28441 agaacgtctt tcgcgccgat atgtgtaacg ctgacgtaaa attgggcgat ctgcttattc
    28501 atgaaggatc ggcgaaagat gcgcagaaat tcgcagccaa agtctttcat gccgataaaa
    28561 cctattttgt gctgaacggc acatcggcag cgaataaagt ggtgacgaat gcgctgttaa
    28621 cgcgtggcga tctggtgctc ttcgaccgta acaaccataa gtcgaatcat cacggcgcgc
    28681 tgattcaggc gggggcgacg ccggtctatc tggaagcttc acgcaacccg tttggtttca
    28741 ttggcggtat tgatgcgcac tgttttaatg aagagtatct tcgccagcaa attcgcgacg
    28801 ttgcgccaga aaaagccgag ctgccgcgcc cgtttcgcct ggcgattatt cagctgggaa
    28861 cctatgacgg aactgtctat aacgcccgtc aggtgatcga taccgttggg catctgtgtg
    28921 attacattct gtttgattcc gcgtgggtcg gttacgaaca atttatcccg atgatggcgg
    28981 atagctcgcc gctgctgtta gaacttaacg aaaacgatcc ggggattttt gtgacccagt
    29041 cggtgcacaa acagcaggct ggattctcac agacgtcgca gatccataaa aaagataacc
    29101 atatccgcgg acaggcgcgt ttttgcccgc ataagcggtt gaataacgcc tttatgctcc
    29161 atgcttctac cagccctttc tatccgctgt ttgctgcact ggatgttaac gccaaaattc
    29221 atgaagggga gagtgggcgt cggctgtggg ctgagtgcgt tgagttgggt attgagtcgc
    29281 gcaaggctat tcttgcgcgc tgtaagctgt tccgcccgtt tatcccgccc gttgttgatg
    29341 gcaaattgtg gcaggattat ccgacatctg tgttagccag cgaccgccgt tttttcagtt
    29401 ttgagccggg ggcgaagtgg cacggctttg aaggatatgc cgcggatcag tattttgttg
    29461 atccgtgcaa gctgttactc actacaccag gtatcaatgc cgaaactggc gaatatagcg
    29521 actttggcgt tccggcgacg attctggcgc actatctgcg tgagaacggc attgtgccgg
    29581 agaagtgcga tctcaactcc attctgttct tattaactcc ggcggaaagc cacgagaagc
    29641 tggcacaact ggtggcgatg ctggcgcaat ttgaacagca tattgaggat gactcgccgc
    29701 tggctgaggt gttgccgagc gtttataaca aatatccggt gcgctatcgc gactacaccc
    29761 tgcgccagtt gtgtcaggag atgcacgatc tgtatgtcag tttcgacgtc aaagacctac
    29821 aaaaagcgat gttccgccag cagagtttcc cgtcagtggt gatgaacccc caggatgcgc
    29881 atagcgctta tattcgcggt gaagtggagt tggtgcggat tcgtgatgcc gaagggcgaa
    29941 ttgcggcaga aggggcgttg ccttatccac ctggcgtgct ttgcgtggta cccggggaag
    30001 tctggggtgg ggcggttcaa cgttatttcc ttgcactgga agaaggggtg aatttgttgc
    30061 cgggattttc gccggagctg caaggtgttt atagcgaaac cgatgcggat ggcatgaaac
    30121 ggttgtacgg ttatgtgttg aagtaagaat aaaaaaaacg ggtcaccttc tgg
//
LOCUS       DAZGVQ010000049        29884 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000049 DAZGVQ010000000
VERSION     DAZGVQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.049"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            110..1693
                     /gene="yegH"
                     /locus_tag="ACHLAG_002090"
     CDS             110..1693
                     /gene="yegH"
                     /locus_tag="ACHLAG_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP0655326.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(1967..3820)
                     /gene="asmA"
                     /locus_tag="ACHLAG_002091"
     CDS             complement(1967..3820)
                     /gene="asmA"
                     /locus_tag="ACHLAG_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFP0655327.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILNAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3842..4423)
                     /gene="dcd"
                     /locus_tag="ACHLAG_002092"
     CDS             complement(3842..4423)
                     /gene="dcd"
                     /locus_tag="ACHLAG_002092"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFP0655328.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4515..5156)
                     /gene="udk"
                     /locus_tag="ACHLAG_002093"
     CDS             complement(4515..5156)
                     /gene="udk"
                     /locus_tag="ACHLAG_002093"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFP0655329.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5473..8788
                     /gene="dgcE"
                     /locus_tag="ACHLAG_002094"
                     /pseudo
     CDS             5473..8788
                     /gene="dgcE"
                     /locus_tag="ACHLAG_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(8827..9684)
                     /gene="alkA"
                     /locus_tag="ACHLAG_002095"
     CDS             complement(8827..9684)
                     /gene="alkA"
                     /locus_tag="ACHLAG_002095"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFP0655330.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDMARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            9818..11170
                     /gene="yegD"
                     /locus_tag="ACHLAG_002096"
     CDS             9818..11170
                     /gene="yegD"
                     /locus_tag="ACHLAG_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0655331.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11183..13123)
                     /gene="yegI"
                     /locus_tag="ACHLAG_002097"
     CDS             complement(11183..13123)
                     /gene="yegI"
                     /locus_tag="ACHLAG_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFP0655332.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEQLRQAAEKLAQAQADLSRC"
     gene            complement(13120..13881)
                     /gene="pphC"
                     /locus_tag="ACHLAG_002098"
     CDS             complement(13120..13881)
                     /gene="pphC"
                     /locus_tag="ACHLAG_002098"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFP0655333.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(13878..14537)
                     /gene="yegL"
                     /locus_tag="ACHLAG_002099"
     CDS             complement(13878..14537)
                     /gene="yegL"
                     /locus_tag="ACHLAG_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP0655334.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIA
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14758..14817)
                     /gene="ibsA"
                     /locus_tag="ACHLAG_002100"
     CDS             complement(14758..14817)
                     /gene="ibsA"
                     /locus_tag="ACHLAG_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFP0655335.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15090..15146)
                     /locus_tag="ACHLAG_002101"
     CDS             complement(15090..15146)
                     /locus_tag="ACHLAG_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP0655336.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            15425..16672
                     /gene="mdtA"
                     /locus_tag="ACHLAG_002102"
     CDS             15425..16672
                     /gene="mdtA"
                     /locus_tag="ACHLAG_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFP0655337.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLIALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKVGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            16672..19794
                     /gene="mdtB"
                     /locus_tag="ACHLAG_002103"
     CDS             16672..19794
                     /gene="mdtB"
                     /locus_tag="ACHLAG_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFP0655338.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSAHLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            19795..22872
                     /gene="mdtC"
                     /locus_tag="ACHLAG_002104"
     CDS             19795..22872
                     /gene="mdtC"
                     /locus_tag="ACHLAG_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFP0655339.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            22873..24288
                     /gene="mdtD"
                     /locus_tag="ACHLAG_002105"
     CDS             22873..24288
                     /gene="mdtD"
                     /locus_tag="ACHLAG_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0655340.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNCFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            24285..25688
                     /gene="baeS"
                     /locus_tag="ACHLAG_002106"
     CDS             24285..25688
                     /gene="baeS"
                     /locus_tag="ACHLAG_002106"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFP0655341.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            25685..26407
                     /gene="baeR"
                     /locus_tag="ACHLAG_002107"
     CDS             25685..26407
                     /gene="baeR"
                     /locus_tag="ACHLAG_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFP0655342.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            26598..26930
                     /gene="yegP"
                     /locus_tag="ACHLAG_002108"
     CDS             26598..26930
                     /gene="yegP"
                     /locus_tag="ACHLAG_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFP0655343.1"
                     /translation="MAGWFELIKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKVNGT
                     SQTVKDNT"
     gene            27139..27435
                     /locus_tag="ACHLAG_002109"
     CDS             27139..27435
                     /locus_tag="ACHLAG_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP0655344.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            27437..27733
                     /locus_tag="ACHLAG_002110"
     CDS             27437..27733
                     /locus_tag="ACHLAG_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0655345.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            27836..29197
                     /gene="yegQ"
                     /locus_tag="ACHLAG_002111"
     CDS             27836..29197
                     /gene="yegQ"
                     /locus_tag="ACHLAG_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFP0655346.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29527..29844)
                     /gene="yegR"
                     /locus_tag="ACHLAG_002112"
     CDS             complement(29527..29844)
                     /gene="yegR"
                     /locus_tag="ACHLAG_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFP0655347.1"
                     /translation="MKKIAAISLISIFIVSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT         6902 a         7976 c         7889 g         7117 t
ORIGIN      
        1 ccctcaaata atatgagata aatagtgctg caacattgca tttttgcccc gatttatcca
       61 tgatcgaatt gtgacatttg tcatacaacg aataggtttt gtacttacta tggaatggat
      121 tgccgatccg tctatctggg ccgggttaat cacgctgatt gtgatcgaac tggtcctcgg
      181 cattgataac ctggtcttta ttgccatcct cgccgaaaaa ctaccgccga agcagcgcga
      241 ccgcgcacgg gttaccgggc tgctgctggc gatgttaatg cgcctgttac tgctggcgtc
      301 aatctcctgg ctggtcaccc tgacgcaacc gctgttcagc ttccgctcgt ttacctttag
      361 cgcccgcgac ttaatcatgc tgtttggtgg tttcttcctg ttgttcaaag ccacgatgga
      421 gctgaacgaa cggctggaag ggaaagacag caataatccc acacaacgca aaggtgcgaa
      481 gttctggggg gtggtgacac aaattgtggt actggacgcc atcttctcac tcgactcagt
      541 gattaccgcc gtcgggatgg tcgaccattt actggtcatg atggccgccg tggttatcgc
      601 tatcagcctg atgttgatgg ccagcaagcc gttaacgcaa tttgttaaca gtcacccgac
      661 catcgttatt ctctgcttaa gcttcctgtt gatgattggc tttagcctgg tggcagaagg
      721 tttcggcttc gtcattccga aaggctacct gtacgctgcc attggtttct cggtgatgat
      781 cgaggcgctc aatcagttgg ctatctttaa ccggcgacgt tttctttccg ctaaccagac
      841 gctacgccag cggactaccg aagcggtaat gcgcctgctt agcgggcaaa aagaagatgc
      901 ggaactggac gccgaaaccg cgtccatgct ggtggatcat ggtaaccagc agatctttaa
      961 tccgcaggaa cggcggatga ttgagcgggt acttaatctt aaccagcgta ctgtcagcag
     1021 cattatgacg tcgcgccacg atattgagca tatcgatctc aacgcgccgg aagacgagat
     1081 ccgccagttg ctggagcgaa atcagcatac gcggctggtg gttaccgacg gtgatgacgc
     1141 agaagatttg ctcggtgttg ttcacgttat cgacctgcta caacagtcac tgcgtggcga
     1201 accgctcaac ctgcgggtgt tgattcgcca gccgctggtg ttcccggaaa ccttgccgtt
     1261 gttacctgcc ctggagcagt tccgtaatgc ccgcacgcac tttgcttttg tggtggatga
     1321 gtttggctcg gtggaaggga ttgtgacatt aagtgacgtc actgaaacca ttgccggtaa
     1381 cttaccgaac gaagtggaag agatcgacgc ccgccatgat attcagaaga atgcggacgg
     1441 ttcctggacg gcgaatggtc atatgccgct ggaagatctg gtgcaatatg tgccgctgcc
     1501 gctggatgaa aaacgtgaat atcacaccat tgccgggctg ttgatggaat atttacagcg
     1561 tattccaaaa cctggcgaag aagttcaggt gggggattat ttgcttaaaa cgttgcaggt
     1621 ggaaagccat cgcgtgcaga aggtgcagat tattccgctg cataaggatg gcgagttgga
     1681 gtacgaggtg tgatggtatc ggtcatttta cccgatagcc gaaaggaata agatgaggga
     1741 accgcgccag cggcgattta ccgccgggag cccgggttgc cagggtggtg gcgattgagc
     1801 caccctggca cgttcaccgg cttgagcgtt ccatagtagt gaggaacatc aagtgaacgg
     1861 aacagccttc gatcttacat attcagaata taatggtgcc gccgcgaacg cctgatgcga
     1921 cgctttacgc gtcttatcag gcctacagga caggtcacct ggtaacctac atcttctcca
     1981 gcaacttctt cacatctttg ccattgcggg aatctttatt ccgctcggcc cagtcattca
     2041 ggcgacgttt cgcttcgtcc tgtagatgtt tgcgcagtaa ctgatccact tgcagactgt
     2101 aattgagttg ctgccagttg ccataaaccc gcagcggtac tggcgtttct ttgaggaaat
     2161 caatcagttt gctttccccg ttccagccac ccacgacccg aatatcaaac tgggtgtcgc
     2221 aggtttgatc tgccagattc aacatgcctt cccctgtcag cgccagcaca ggcgattgac
     2281 cttgcatgtc gtttaacgtc acgacgccat ccttcaacgt caaatcggtg gtaaagcggt
     2341 caagccgcgt cacgttatca aagttttcag cggccttcac atcaccgcca ttacgctcta
     2401 ccgcttgctg aatcatctgc tggaagttca tcccttccat gcgcgtgtcg gtcatttcga
     2461 catgtgcctg tccttgccag ttgtggcgga atgcgtcggc atctatgtca gcaccggaga
     2521 aatcaccagc cagtgacatt tttccggtca gcgaaatggg gtagttaaag gcattcagaa
     2581 tggtaccaat ctcaacgttt tccagccgtg gctggaagtt tatccgcgga tttattgatg
     2641 tcgcgtccag cgtgcccggc agtgaaacct gtccaccgtt aagcttgccc tgcagttgag
     2701 taatttccag caaacccgac ttgttggtca tttgcgtggc aacatctgta aaattcattc
     2761 cgcgccagcg cacgttactg gcctgcaaca aaatatcagc cgtaaagcct tgcagtccct
     2821 gatatgccgg ttcatcaata cgcgaagaaa tgaccgggcg cggcaatgtt gattggctct
     2881 gcccctgctg cgcggcaccg ttttcaccat tcgctgtttc attaagcggg atgaggttgt
     2941 caagattcag ttgcgggaat tgcagtctca gctgccattc aggtttctct gtcagcgtga
     3001 cctgtgcttg cccgctcagc gtgctgtcat tggcggtcaa gctgatttgg ttaaatgaaa
     3061 ggcgtttatg tgactcctgc cactgggcct ggaaactccc ctgcccctga ataccttgtt
     3121 ttggtaaatc tgcaccctgc aactgccagt taatttgttc aatagccgcc gttaaatcat
     3181 gcggataatc agaagcatct accgtaccgt taagggatat cgtgagatcg cgctgatcgc
     3241 gattaacccg cccggagaac tcaaatgaac cacgatgttg gggatcttgt tccatttgca
     3301 ggcgaatatt gcggattgtc acctgctcgt catcttcatg ctggaacacc agcacgctgt
     3361 ccgccacctt aagactggat atatcaaacg accatccgcg atcgtctgac agatccggca
     3421 aggtattgtc gcgcggtgca accggagcgt cttcactgcg caccgcttcc gtctgcggcg
     3481 tcagttggat cactgcccct tttagcatca cctgcttaac gctcagttga tgactcagta
     3541 gtggtaaaag cgccacgtcc agacgcatgt tgtcggcgcg aaccagtggc tggcttgcgc
     3601 cctgggcggt gagagacatt cgcccggaga ggatactaag ctgcggccag acgtgccaac
     3661 gcagtggccc gtcgagctgc aattgataac cgctacgtgc agcaacttgc ttgaccatat
     3721 agtcgcggaa atcattcgga ttcaccagca acactaacgc agataacccg gccaccagca
     3781 cgaccaggag tatcatcagc gtcgtcagaa atcgtctcat ggtatcctca atgggcctga
     3841 attagtcttt atcgattcgg ctggctaccg cgccctgctg gttgcgatat ttcgcatctt
     3901 cacggcggtt gtaaggtcgc gccgccgggc cggaaagcgg ctcaaagctc agcgcaccaa
     3961 ttaacatgcc cggacgcagc gccagcggca gcttaccgga gttgtagaac tccagcacaa
     4021 tgcaaccaga ccagcccgga tcgatacggt gcgcggtgac gtgcaccatc agccccagac
     4081 gcgccaaaga ggaacgcccg tccagccagc ccaccagatc ggctggcagc gtcaccgact
     4141 ccagcgtcac cgccagcgcc agctctcctg ggtgaagata gaacgcctcg ccctcgtcga
     4201 gaacgatctc atcgctcatc acgcggtcaa gcgcggcgct cacttcatct ttgggaccgc
     4261 tcagatcgat aaacgctgcc gtgtgaccac ggaaggtacg aaatttattg cccaagcgta
     4321 catccaccgt cgcgccgtta atacgctcca ctggcggacg tgggttgatc gacaaacggc
     4381 cttcatcaag ccaggcttca atatctcggt cacacagacg catggcattt tctcctttcg
     4441 cgcatcactc ccttaacgcc aattacgtca agggcatacc aggttatcac tgaacggtac
     4501 acaatttatc aagcttattc aaagaactga cttattttcg ctttcaatat atcgatcgcg
     4561 atgcggtttt tcccgccgcg cggcacgata atgtccgcat attgtttaga aggctcaatg
     4621 aattgcagga acatcgggcg cacggttttt tgatattgcg ccatcacgga atccattgaa
     4681 cgcccacgct cgttaacgtc acgcttgatg cggcgcatca ggcagatatc cagcggggta
     4741 tcaacgaaaa tggagaagtt aagttcgtca cgcaaacgcg catccgtcag caacaaaatg
     4801 ccttcgagaa tgatgacctt cttcggctca accgtcaccg tttctttcat acgcgtatgt
     4861 tcaacatagc tgtaaaccgg caggtcaatt gccgagccgc gtttcaacgc ttgtaaatgc
     4921 tcaagcagca ggctgtgatc catcgcgctg ggatggtcgt agttggtctt aacgcgttct
     4981 tccatcgaca gatggctttg atctttgtaa tagcagtctt cgggaattac gccgatgtgt
     5041 tcatcaccga cttgctcacg taattcacga taaagggtac tggcaataag actcttgccg
     5101 gaagccgatg cgccagcgat accgataatg acgcactgat gagactgatc agtcatatat
     5161 ttagcgacct gattaacctg gatgttagga aggggcgacg aagcgccaaa cgcggcaatt
     5221 atagggattt catccgcctg ataccagtcg aatagcgctg ccgcgcgctc agagtttatt
     5281 gttgacgaag aattcccggt ggcaaattac gttgatcagt tttatgtaag gtaaaaaatg
     5341 ttatacgcag ttgcgcaaat tatccgcctt tacgtcactt tatgagcaat tcgcatataa
     5401 aatgtaaaac ttttgtacta gcataaacac agaaacgaat actggcgacc tggtcttgcg
     5461 gataaagcgg taatgagcaa acaatcacag catgtattaa ttgccctgcc ccacccgctg
     5521 cttcacctgg tcagtttagg tttagtctcg tttatcttta cccttttctc gcttgagctt
     5581 tcgcagtttg gcacccaact cgccccactg tggttcccga cgtccatcat gatggtggcg
     5641 ttttatcgcc atgccgggcg catgtggccg ggaattgcgc tgagctgttc gctgggaaat
     5701 atcgccgcat ccatcctgct tttttccacc agctcgctga acatgacctg gacgaccatc
     5761 aatattgttg aagccgtggt cggggcagtg ctgctacgta aattgctgcc gtggtataac
     5821 cccttgcaaa atctggctga ctggctgcgt ctggcactcg gcagcgccat tgttccgcct
     5881 ctgttggggg ggtctggttg tcctgctgac ggccggagac gatcctctca gggcattttt
     5941 gatatgggta ctgtcagaat ccatcggcgc tctggcactg gtgccgctgg gattgttatt
     6001 taaaccacac tatctgctgc gccatcgcaa cccacggttg ctttttgagt cgctgctcac
     6061 gttagccatc acactgacgt taagctggct ttcgatgctg tatctgccgt ggccttttac
     6121 tttcattatt gtgctgttga tgtggagcgc cgtgcgcctg ccacgaatgg aagccttttt
     6181 gatcttcctt accacggtga tgatggtgtc gctgatgatg gccgcggatc cctccctgct
     6241 tgctacgccg cgtacatacc tgatgagcca tatgccgtgg ctaccgtttt tgctgatcct
     6301 gctgcccgcc aacatcatga cgatggtgat gtatgccttt cgtgcggaac gcaaacacat
     6361 ttccgaaagc gaaacccgtt ttcggaacgc catggaatat tccgctatcg gtatggcgtt
     6421 agtgggcacc gagggacaat ggctgcaaac caacaaagcg ctctgccagt ttctcgggta
     6481 cagtcaggaa gagctgcgcg gactcacctt tcagcaactg acctggccgg aggatctcaa
     6541 taaagatctc caacaggttg aaaagctgat aagcggtgaa ataaacacct attcaatgga
     6601 aaaacgctac tacaaccgca atggcgatgt tgtctgggcg ttgcttgccg tctcactggt
     6661 gcgccacacg gatggcacgc cgctctattt tatcgctcag attgaagaca ttaacgagct
     6721 aaaacgcacc gaacaggtga atcagcaact gatggagcgc atcacgctgg ctaacgaagc
     6781 gggcgggatt ggcatctggg agtgggagtt gaagccgaat atttttagct gggataagcg
     6841 gatgttcgag ctgtatgaaa ttcctccaca tatcaaaccg aactggcagg tgtggtacga
     6901 gtgcgtgctg ccggaagatc gccagcatgc cgaaaaagtg attcgtgatt cgttgcaatc
     6961 acgctcgccc tttaaactgg aatttcgcat taccgtaaaa gacggtattc gccatatccg
     7021 cgccctcgcc aaccgggtac tgaataaaga aggcgaagtc gaacgcctgc tcggcattaa
     7081 tatggatatg actgaggtta aacagcttaa cgaggcattg tttcaggaaa aagagcgcct
     7141 gcacataacg ctggactcca tcggcgaagc cgtggtctgt attgatatgg caatgaaaat
     7201 tacctttatg aatccagtgg cggagaagat gagcggctgg acgcaggaag aagcattagg
     7261 tgttccgctc ctgacggtgt tgcatattac ctttggcgac aatggtcccc tgatggagaa
     7321 catttacagt gccgacacct cacgttccgc gattgaacaa gatgtggtgt tgcactgccg
     7381 gagcggcggc agctacgacg tgcattacag tattacgccg ttaagtactc tggacggcag
     7441 caatattggt tcggttctgg tgattcagga cgtcaccgaa tcacgcaaaa tgctacgcca
     7501 gctgagctac agcgcctccc atgatgcact aacgcatctc gccaatcgcg ccagttttga
     7561 gaagcaactg cgtatcctgc tgcaaacggt aaacagtaca catcagcgac atgccctggt
     7621 gtttatcgat cttgatcgct ttaaagcggt gaatgacagc gccgggcacg ccgctggcga
     7681 cgctttactg cgcgaactgg cgtcgttgat gctgagcatg ctgcgctcca gcgacgtgct
     7741 ggcgcggctc ggcggtgatg aatttggtct gctgctgcca gactgcaatg tcgaaagtgc
     7801 gcgttttatc gctacacgta ttatcagtgc tgtgaatgac tatcacttta tctgggaagg
     7861 acgtgtacat cgggtaggtg ccagtgccgg gattaccttg attgatgaca acaatcatca
     7921 ggcggctgaa gtgatgtcgc aggctgatat cgcctgttat gcctccaaaa atggtggccg
     7981 tggccgggtg acgatttacg aaccgcagca agctgccgca catagcgaac ggacggcgat
     8041 gtcgcttgat gaacagtggc ggatgattaa agagaatcag ttgatgatga tcgcccacgg
     8101 tgtcgcttcg ccgcggatcc cggaagcgcg taatttgtgg ctgatttcgc ttaaactctg
     8161 gagttgcgaa ggcgagatta ttgatgaaca aaaatttcgt cgtagcttca gcgatccggc
     8221 acttagccat gctcttgacc gacgggtatt ccacgaattt ttccagctgg ccgcaaaagc
     8281 ggttgccagt aaaggcataa gcatcgccct ccccctttcc gttgccggtt tgagtagcgc
     8341 cacgctggtg aatgatctgc ttgagcagct ggaaaatagc cctctaccac cacggttatt
     8401 acatctgatt attccggcag acgcgatttt agatcacgca gaaagcgtgc aaaaactgcg
     8461 gctggcggga tgtcggatag tgctcagcca ggtgggccgc gatctgcaaa tcttcaactc
     8521 gctgaaagcg aatatggcag attacctgct acttgatggt gagttatgcg ccaacgtgca
     8581 gggtaatttg atggatgaga tgctgattac gattattcag gggcacgctc agcgactcgg
     8641 gatgaaaacc atcgccgggc cagtcgtttt acccttagtg atggatacgc tttctggcat
     8701 cggcgtcgat ctgatttatg gtgatgtgat tgccgatgcc caaccgctgg atttgctggt
     8761 gaatagcagt tatttcgcga ttaactgagg gagcgtttgc ccattgcctg atgcgacgca
     8821 tcaggcctac aattcgtccg ttccgtctgg ttgccagcct tccgtatacc agatatgcaa
     8881 cagcgcataa gaacgccagg gcttccagcg ctcggcataa cggcggattt gtgccggtgt
     8941 catccccgga aatcgctgtt taatcagata atcatccggc agaaaaacat ctttcgcctg
     9001 ccagccacgc aaagcgaaat aattcgccgt ccagcgcccg atacccggaa aagtttgcag
     9061 cgttttcatc gcctgctcca catcgcccgg aattgtcatt ggtaagctgc cctccagcgc
     9121 cgcatttgcc agatgaatca gtgcctctgc ccgtttcaac ggcatgccta acgcttttaa
     9181 tgcctgcggg tcggctgctg ccagccgctg aggcgtgggg aagcagatat actccggaaa
     9241 atcatccagc cgttcgccat aaagctgtac cactttggca gtcaattttg ccgccatcgc
     9301 cacgctcacc agttggccta aaatcgcccg cacaccctgc tcaaaagcat caacacagcc
     9361 gggtaaacgc aatccgggcc gcgccgcgcc taacttgccc aacgcaccgt taacaatctg
     9421 cgggttacat tgcagatcaa gcaggcggct cattttcgcc agacactcag cggcaacagg
     9481 ttctaaacct gcacttaaat ttatgtgcag agtatgacgg gccatatccg gaatagcagt
     9541 cactacgccg cgatattcgc ccaccgccag actacgggca taataatcgt cagcgaccgt
     9601 ttccacgcca ctcactgcac gggcggcgag aaatcccaac atccacgacc agtcatacgg
     9661 cggctgccag ttcagggtat acatcgcatc tccttattca tccgctttca gcataaacgt
     9721 tattcagacg ctacgctttg ctttcatatt ccggttgtcg cgacggcaac atttcgctaa
     9781 agtcacgccc cttcttcacc ggcatgggga ttaattcgtg tttattggtt ttgattacgg
     9841 tacagcaaac tgttcagtgg cggtcatgcg tgacggtaaa ccgcaattac tgaaaatgga
     9901 aaacgacagc acgctgctgc cttcaatgct ttgcgcgcca acgcgtgaag cagtaagtga
     9961 atggctgtac cgccatcatg atgttccggc agacgacgat gaaacgcagg cgctgttacg
    10021 tcgggcgatt cgttataacc gcgaagaaga tatcgatgtt acggcgaaaa gcgtgcagtt
    10081 cggtctttcc tcactggctc agtacattga tgatccagaa gaagtgtggt ttgtgaaatc
    10141 accaaaatcg ttcctcggtg ccagcggctt aaaaccgcag caggtagcgc tgtttgagga
    10201 tctggtctgc gcaatgatgt tgcacattcg ccagcaggcg caggcgcagc tgccagaagc
    10261 gattactcag gcggtgattg gtcgcccgat caacttccag gggctgggcg gcgatgaagc
    10321 aaacgcccag gcgcaaggga ttctggaacg cgcggcgaag cgtgccggat tcagggacgt
    10381 ggtattccag tacgagccgg tcgcggctgg gctggattac gaagccacct tgcaggaaga
    10441 aaaacgggtg ctggtggtgg atatcggcgg tggtacgact gactgttcat tgctgctgat
    10501 ggggccgcag tggcgttcgc gtctcgatcg tgaagccagt ctgctgggtc acagtggttg
    10561 tcgtattggc ggtaacgatc tggatatcgc cctggcgttt aaaaacctga tgccactgct
    10621 gggtatgggt ggcgaaaccg aaaaaggcat cgccctgccg atcctgccgt ggtggaatgc
    10681 ggttgccatc aacgacgtac ctgcgcagag tgatttctac agtagtgcca acggtcgtct
    10741 gcttaacgat ctggtacgcg atgcccgcga accggaaaaa gtggccctgt tacagaaagt
    10801 ctggcgtcag cgtttaagct atcgcctggt gcgtagcgca gaagagtgca aaattgctct
    10861 ttcaagcgta gcggaaaccc gcgcctcact gccgtttatc agcaatgaac tggctacgct
    10921 gattagccag cgagggctgg aaagcgccct cagccagcca ctggcgcgga ttctggaaca
    10981 ggtgcaactg gccctggata acgcccagga aaaaccggac gttatctacc tgaccggcgg
    11041 tagcgcccgt tctccgctga ttaaaaaagc gctggcagaa cagttgccgg gcattccgat
    11101 tgcaggcggc gatgactttg gctccgtcac cgccgggctg gcacgctggg cggaagtggt
    11161 gtttcgttaa ttgaaaaatg gctcagcagc ggcttaaatc tgcctgcgcc tgagccagtt
    11221 tttctgctgc ctgacgtaac tgttccatca acggcatggt ccgtgctgga gcatgaaggc
    11281 ggaatcgctg caactctgtc gcgccgactg tcaacgccga ttccagccga tgtcgtttgg
    11341 cggtcatttt cgccattacc gcttgtctgt ctgctggcgt tatcgcttca ttcggcctga
    11401 acacaaaacg gcgttcacag ctcgctttcc agtcgataac cgcctgggtc agatggtcgc
    11461 caaacccttt cacttgctta acgccacgac gggtaacatc tgcggctgtt tcaataccaa
    11521 aagaccgtaa cgccgcttta cgcgcagggc caacgccggg aatagaggca acatcaataa
    11581 aaaatccctc cagaaacttc tgcttctgcc gttcccttgc agtgtcatga agtgctgcca
    11641 gagcgcgttt ctcttcttcc ggtaacccga gaatttcgtc cttcattttt tcgagcatcg
    11701 tccgtttggc gataaaacct tccaggccgc ctacctgctg gatctggcgg actaaatggt
    11761 catagtccat tttcgcacga ttaaatgcct cacggcgttg ctgaacttct gctttgtacg
    11821 ctttgcttgt cagactgcca ataatccaga tagccgccag cacaggaacc aaaataatat
    11881 aacgcggttc tgcctgaagg ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa
    11941 gcagaatgat gtattcgcgc cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga
    12001 aatgatcggg taatggcagt tgcaatgctg gcggtgctac tgacgccatc accatcgccc
    12061 agacttttgc cagcacaaaa tccccgccgg tggtaatgac ctcttcgccg agatcaataa
    12121 aatagataac accttgatta tccagcgcac accatgggca gtcagccaaa tgaccgggat
    12181 aaacatgcat tgccgaaacg gtacatttct ttaactgttg gcgcagagaa tcaagtgccg
    12241 ctacccacgc tttagccgtc ggacgcccgg ttgccacgcc actttccgtg aatgcctgct
    12301 gaaacatggc ttcaacatca ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt
    12361 ttaaaccacg tcgctgatta tctgacgcgt aggcataacg gaaatgggta atatccgtct
    12421 ccagcgcatt acccgcatca gagataagcg gcacaccaga ataaggatgc cggccaccaa
    12481 acaagacgtg aaaaatcagc aacgcaaggc caaaattatc gtgattttct gtgcgttcaa
    12541 agccgacaaa tgacgacaag gtttgtagct ctggcggcgt aaaatgcgac acgccgactt
    12601 cgcataaatg cagtgtgcca ttggcgttaa tctgaaagga gtcgctatca atcagtacca
    12661 ctttgctgtc gcgacctacc ataaaactgt tctggtttac gtcacccacg acgtgcccgt
    12721 gctcgtgaac cgtagcaaaa gatgaagcaa tattgcgcgc aacatagagt agaaaatccc
    12781 acgcacaatg agggtaacgt tgacgacgat gtgccgggct atagatcata tgaatcggtt
    12841 ctttaccaga aacttttggc atcataaaac cgataacttt tccgcctcgt ccaccgtgaa
    12901 gcgttgcctg cggccaggcg acataattca acaattgcgc gtcagctgtc gcagccataa
    12961 aggcaagttt gtcctgtttt aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat
    13021 cgacaaactc attgatatca taaaccgcgc cttcaccgcc tttgcccagt tcacggcctg
    13081 gcgtaacgca ctcaccagta gcagtatata aagtgggttt catggtaacc acagcgccag
    13141 tgctaacgtt ttatcatcat cggtacgctc gtttaccgct gggctggaca aaaactgctt
    13201 taacaattca ggtaataaat cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa
    13261 cggggtaaag aaaggcacat gcggagaatt atccaacata tttaacgcca gtcgctggat
    13321 accatccgta aatgctgcaa ctttatgcac acgctcagtg ctggtgaaag tttccagtct
    13381 ggaaacggca tcttcatcgg taatgaagtg cgtcatattg gcatattcac ccaccatcgg
    13441 cgtaagcggc aattgcagac cgtgaccaaa atccaccacc acgccgccat cgccaatctg
    13501 cataatcaag gtgccattag ccgatgaaat caatcccaga aacgtgcagg caaaatcgcg
    13561 aaccgctaat tctttggcct ctgcctcggc aaaaagtcgc tgacgaacgg tcagcacaat
    13621 atccgtggcg aggatatcat taagccccaa ttccccaccc tgcacttttt gcgacatata
    13681 agccatcgct tcattgacgg cgagcatcgc gccttcgcca ccctgcgaga cgctgcctgc
    13741 accatcagca agaaacatca ccagtaatgg ttgctgatcg tttaaccagg caacctgcat
    13801 ctggcaggca tcctgacaag gtaaatcagc gctaatgtgc gacgttccaa cagcggatgc
    13861 ataaaccaga cgccagctca cactgacgtc cagccttttg gcgcttccag cacaacttcc
    13921 gttcccggcg tggagcggga aaccgaacgc agcgaactgg ataaccagct aaacagttca
    13981 cggaattgta atccttgcag aggcaaaggc tgacgaacgc tgatttgtgc cagcgtcttc
    14041 atatccgccc cctgaacagc gatggaaaag aaggcaaact tcttatcttc ttccccctga
    14101 aacactttgt tggcggcagc ctgccactca tcggttggag cgccatcggt gatcaggaaa
    14161 atccacgggc gataatacga aataccattt gcgcggtact cacgtttcct ctcttcaacc
    14221 atatccaggg ctttggtaat ggcggccccc attggcgtat ccccctgggc aaacaggatg
    14281 ggcgggaaaa agttagcggc gctggtaaag ggttgttcca catgcaccgg gccgaacgtc
    14341 acaataccga gttccactct ttttaaggcc agcgaatcgg caagcagctc atcgcgaaag
    14401 gtaaccaatc cggcgttaag ttcattaatg ggtctgccgc tcatcgagcc agaaacatcc
    14461 agcagcaaaa tgcaggggca acgtggttcc gggttactgg caaaatcgct ggtggcaaac
    14521 gtgatttgtt cgctcataag aaatcctttc ttagagaatt attgaggtcc ggtatcatac
    14581 cgactgcaat aatgaaataa gagcaaacga agaaactttc agaattaggc ccggcggcgc
    14641 agaaaatttt gctattcgcc tcgcaagaac ggagagccgt cgattgacac taagggcgga
    14701 gtgacataat ttcaggagtg agggttaggg agaggtttcc ccctccccct ggtgttctta
    14761 gtaagcctgg aagctaatca ctaagagtat caccagtatg atgacgtgct tcatcataac
    14821 cctttcctta ttaaaagccc tcttctccgg gagaggcttt cccgtttcag cgtcccgctg
    14881 aaatcatcgg cttacctcct ttcgccctga atgcagtcta tcgctaacgc gtgaataaaa
    14941 tagtttcctc tgtgattatt gcgtaacgac tcgcaaagaa tatttgctgg caggatcgca
    15001 gactacaaag cctgcggatt gacaatctta tcgtgaaggc atactttcag gagtgagggt
    15061 agagcggggt ttcccccgcc ctggtagtct tagtaagcgg ggaagcttat gactaagagc
    15121 accacgatga tgagtagctt catcatgacc ctttccttat ttaaggcccc ttcctcggga
    15181 ggggctttcc cgtttcagcg tcccgctgaa atcgtcggct tacctccttt cgccatgcaa
    15241 gcagtctatc gctaacgcgt agataaaata gtttcctgtg ttattactgg atgcgtgctc
    15301 gcaaatgtgc ccgccattca gacgattccc gatagtgttt cataattcct ccatttttct
    15361 cccttattgg ctggctacac tagtatcatt ccgcgaaacg tttcaggaag agaaactctt
    15421 aacgatgaaa ggcagttata aatcccgttg ggtaatcgta atcgtggtgg ttatcgccgc
    15481 catcgccgca ttctggttct ggcaaggccg caatgactcc cagagtgcag ccccaggggc
    15541 gacgaaacaa gcgcagcaat cgccagcggg tggtcgccgt ggtatgcgtt ccggcccatt
    15601 agccccggtt caggcggcga ccgccgtaga acaggcagtt ccgcgttacc tcaccgggct
    15661 tggcaccatt atcgccgcta atactgttac ggtgcgcagc cgcgtggacg gccaactgat
    15721 agcgttacat ttccaggaag gccagcaggt caaagcaggc gatttactgg cagaaattga
    15781 ccccagccag ttcaaagttg cattagcaca agcccagggc caactggcaa aagataaagc
    15841 cacgcttgcc aacgcccgcc gtgacctggc gcgttatcaa caactggcaa aaaccaatct
    15901 cgtttcccgc caggaactgg atgcccaaca ggcgctggtc agtgaaaccg aaggcaccat
    15961 taaggctgat gaagcaagcg tcgccagcgc gcagttgcaa ctcgactgga gccgcatcac
    16021 cgcaccagtc gatggtcgcg ttggtctcaa gcaggttgat gttggtaacc aaatctccag
    16081 tggtgatacc accggaattg tggtgatcac ccagacgcat cctatcgatt tagtctttac
    16141 cctgccggaa agcgatatcg ctactgtagt gcaggcgcaa aaagtcggaa aaccgctggt
    16201 ggtagaagcc tgggatcgaa ccaactcgaa gaaattaagt gaaggcacgc tgttaagtct
    16261 cgataaccaa atcgatgcca ctaccggtac gattaaagtg aaagcacgct ttaataatca
    16321 ggatgatgcg ctgtttccca atcagtttgt taacgcgcgc atgttagtcg acaccgaaca
    16381 aaacgccgtg gtgatcccca ccgccgccct gcaaatgggt aatgaaggcc attttgtctg
    16441 ggtgctgaat agcgaaaaca aagtcagcaa acatctggtg acgccgggca ttcaggacag
    16501 tcagaaagtg gtgatccgtg caggtatttc tgcgggcgat cgcgtggtga cagacggcat
    16561 tgatcgcctg accgaagggg cgaaagtgga agtagtggaa gcccagagcg ccactactcc
    16621 ggaagagaaa gccaccagcc gcgaatacgc gaaaaaagga gcacgctcct gatgcaggtg
    16681 ttacccccga gcagcacagg cggcccgtcg cgcctgttta ttatgcgtcc tgtcgccacc
    16741 acgctgctga tggtggcgat cttactcgcc gggattatcg gttatcgcgc cctgcccgtt
    16801 tcggcgctgc cggaagtgga ctatccgacc attcaggtgg tcacgctcta cccaggtgcc
    16861 agcccggatg tcatgacctc tgccgttacc gcgccgctag aacgccagtt cgggcagatg
    16921 tctggcctga aacagatgtc gtcgcaaagt tccggcggtg cgtcagttat cactttgcag
    16981 ttccagctaa cattaccgct cgatgtcgcc gagcaggaag tgcaggccgc gattaacgct
    17041 gcgaccaact tgttgccgag cgatctgcct aacccgccgg tttacagcaa agtgaacccg
    17101 gcagatccgc cgatcatgac gctcgccgtc acctcaaccg ccatgccgat gacgcaagtg
    17161 gaagatatgg tggaaacccg cgtcgcgcag aaaatctcgc agatttccgg tgtcggactg
    17221 gtgacgcttt ccggcggtca gcgtccggct gttcgcgtca aacttaacgc tcaggcgatt
    17281 gccgccctcg gcctgaccag cgaaaccgtg cgcaccgcca ttaccggcgc taacgttaac
    17341 tcggcaaaag gtagcctcga cggcccttcc cgtgcggtta cgctttccgc taacgaccag
    17401 atgcaatccg ccgaagagta tcgccagcta atcatcgcct accagaacgg cgcgccaatt
    17461 cgtctgggcg atgtcgcaac tgtagagcaa ggtgcagaaa acagctggct cggcgcgtgg
    17521 gcgaacaaag aacaggccat tgtgatgaat gttcagcgcc agcccggtgc taacattatc
    17581 tccaccgccg acagcattcg gcagatgctg ccacagctca ctgagagtct gccgaaatcg
    17641 gtgaaggtga cagtgctttc cgatcgcacc accaatatcc gcgcatccgt cgatgatact
    17701 cagtttgaat tgatgatggc tatcgcgctg gtagtcatga ttatctacct gtttttgcgc
    17761 aatattccgg cgaccatcat tcccggtgtt gctgtaccgc tgtcgttaat cggcactttc
    17821 gcggttatgg tgtttctcga tttttcaatc aataacctga cactgatggc gttaactatc
    17881 gccaccggat tcgtggtcga tgacgccatc gtggtgatcg aaaacatttc ccgctatatc
    17941 gaaaaaggcg aaaaaccgtt ggcggcggcg ctcaagggcg caggtgaaat cggctttacc
    18001 attatctcgc tgaccttctc actgattgcg gtgttgatcc cactgctgtt tatgggcgat
    18061 atcgtcgggc gactgttccg cgaatttgct attaccctgg cggtagcgat tttgatctca
    18121 gcggtggtgt cgctgaccct gacaccgatg atgtgcgcgc ggatgctcag ccaggagtcg
    18181 ttgcgtaaac agaaccgctt ctcccgtgcc tcggaaaaaa tgttcgacag gataatcgcc
    18241 gcctatggtc gtggactggc gaaagtgctg aatcatccgt ggctgacctt aagcgtggca
    18301 ctcagcacgc tgctgcttag cgtgctgctg tgggtgttca ttccgaaagg tttcttcccg
    18361 gtacaggaca atggcattat tcagggcact ttgcaggcac cgcaatccag ctcctttgcc
    18421 aatatggccc agcgacaacg ccaggtcgcg gacgtgattt tgcaggatcc ggcagtgcaa
    18481 agcctgacct catttgttgg cgttgatggc actaacccgt cgctgaacag tgcacattta
    18541 caaatcaacc tcaaaccgtt ggatgaacgt gatgatcggg tgcaaaaagt catcgcccgt
    18601 ctgcaaacgg cggtagataa agtgccgggc gtcgatctct tcctgcaacc aacgcaggat
    18661 ctgactattg atactcaggt cagccgcacc cagtaccagt ttaccttgca ggccacgtca
    18721 ctggatgcgc tcagtacctg ggtgccacag ttgatggaaa aactccagca actgccacag
    18781 ctttctgatg tctccagcga ctggcaggac aaagggctgg tggcgtatgt caatgttgat
    18841 cgcgacagcg ccagccgtct ggggatcagc atggcggatg tcgataacgc cctgtacaac
    18901 gcgtttggtc agcggctgat ttccactatt tatactcagg ccaaccagta tcgcgtggtg
    18961 ctggagcaca acaccgaaaa taccccaggc ctcgcggcgc tggataccat tcgcctgacc
    19021 agcagcgacg gcggcgtggt gccgctaagc tcaattgcca aaattgagca gcgttttgcg
    19081 ccgctctcca tcaaccatct ggatcagttc ccggtaacga ccatctcctt taacgtgccg
    19141 gataactatt cgctgggcga tgcggtgcag gcgattatgg acaccgaaaa gacgctgaat
    19201 ctgccggtgg atatcaccac gcagttccag ggcagcaccc tcgccttcca gtcggcgctg
    19261 ggcagcactg tctggctgat tgtcgcggcg gtggtggcga tgtatatcgt gctcggcatt
    19321 ctgtacgaga gctttattca cccgatcacc attctctcga cgctacccac cgcaggagtc
    19381 ggcgcactgc tggcgttaat gattgccggt agcgaactgg atgtgattgc gattatcggc
    19441 attattttgc tgatcggtat cgtgaagaag aacgccatca tgatgatcga cttcgcgctg
    19501 gctgctgagc gcgagcaagg catgtcgccg cgcgatgcca tctaccaggc ttgtctgttg
    19561 cgttttcgtc cgatcctgat gaccactctg gcggctctgc ttggcgcgct gccgctgatg
    19621 ttgagtaccg gggtcggcgc ggaactgcgt cgtccgttag gtatcggcat ggtcggcggc
    19681 ctgattgtca gccaggtgct gacgctgttt accacgccgg tgatttatct gctgttcgac
    19741 cgcctggcat tgtggaccaa aagtcgcttt gctcgccatg aagaggaggc gtaagtgaag
    19801 ttttttgccc tcttcattta ccgtccggtg gcgacgattt tactgtcggt tgccattacc
    19861 ctgtgcggca tactgggctt ccgtatgctg ccggtcgccc cgctgccgca ggtcgatttt
    19921 ccggtgatta tggtcagcgc ctcgctgccc ggtgcgtcac cagaaacaat ggcgtcttcc
    19981 gttgccacgc cgctggagcg ctcacttggg cgcattgccg gagtcagtga aatgacctcc
    20041 agcagttcgc tcggcagcac gcgtattatt ttgcagtttg attttgaccg ggatatcaac
    20101 ggcgcggcgc gcgatgtgca ggcggcgatc aacgctgcac aaagtttgct gcccagcggg
    20161 atgcccagcc gtccgaccta tcgcaaagct aacccgtcgg atgcgccaat tatgatcctc
    20221 acgctgacat ccgatactta ttcgcagggt gaactgtacg atttcgcctc gacgcagctg
    20281 gctccgacga tttcgcaaat cgacggtgtt ggtgatgtcg atgtcggcgg cagctcactg
    20341 cccgccgtac gcgtcgggct gaatccgcag gcgctgttta atcagggcgt ctcgctggac
    20401 gacgtacgca ctgccatcag caatgccaac gtgcgtaaac cgcagggggc gctggaagat
    20461 ggcactcacc gctggcagat ccagaccaat gatgagctaa aaaccgccgc cgaatatcag
    20521 ccgctgatta ttcattacaa caacggcggc gcggttcgtc tgggcgatgt ggcgacagtg
    20581 accgactctg tacaggatgt acgtaacgcc gggatgacca atgccaaacc ggctattttg
    20641 ctgatgatcc gcaagctgcc ggaagccaat attatccaga cggttgacag catccgggca
    20701 aaattaccgg agttgcagga aaccattccg gcggcgattg atctgcaaat tgctcaggat
    20761 cgctccccca ccattcgcgc ctcgctggaa gaagtcgagc aaacgctgat tatctcggtg
    20821 gcgctggtga ttctggtggt gtttttattc ctgcgctcgg gtcgcgcaac tattattccc
    20881 gccgttgcgg tgccggtttc gctgattggt acgtttgcgg cgatgtacct gtgcggtttc
    20941 agtctcaata atctttcgtt aatggcgctc accatcgcta ctggtttcgt ggtggatgac
    21001 gccatcgtgg tgctggaaaa cattgcccgt catctggaag cgggaatgaa accgttgcaa
    21061 gccgcactgc aaggtactcg cgaagtcggt tttacggtgc tgtcgatgag tctgtcactg
    21121 gtggcggtgt tcctgccgct gctgttgatg ggcggattgc cgggccgact gttacgcgaa
    21181 tttgccgtga cgctttctgt cgccattggt atttcgttgc tggtttctct gacattaacg
    21241 ccaatgatgt gtggctggat gctgaaagcc agcaagccgc gcgagcaaaa gcgactgcgt
    21301 ggttttggtc gcatgttggt agccctgcaa caaggctacg gcaagtcact gaaatgggtg
    21361 ctcaatcata cccgtctggt gggcgtggtg ctgcttggca ccattgcgct gaatatctgg
    21421 ctgtatatct cgatcccgaa aaccttcttc ccggagcagg acactggcgt gttgatgggc
    21481 gggattcagg cggatcagag tatttcgttt caggcgatgc gcggtaagtt gcaggatttc
    21541 atgaaaatta tccgtgacga tccggcagtg gataatgtca ccggctttac aggcggttcg
    21601 cgagtgaaca gcgggatgat gtttatcacc ctcaagccac gcgacgaacg cagcgaaacg
    21661 gcgcagcaaa ttatcgaccg tctgcgcgta aaactggcga aagaaccggg ggcgaatctg
    21721 ttcctgatgg cggtacagga tattcgcgtt ggtgggcgtc agtcgaacgc cagctaccag
    21781 tacacgttgt tatccgacga cctggcggca ctgcgcgaat gggagccgaa aatccgcaaa
    21841 aaactggcga cgttgccgga actggcggac gtgaactccg atcagcagga taacggcgcg
    21901 gagatgaatc tggtttacga ccgcgacacc atggcacggc tgggaatcga cgtacaagcc
    21961 gccaacagtc tgttaaataa cgccttcggt cagcggcaaa tctcgaccat ttaccagccg
    22021 atgaaccagt ataaagtggt gatggaagtg gatccgcgct atacccagga catcagtgcg
    22081 ctggaaaaaa tgttcgttat caataacgaa ggcaaagcga tcccgctgtc gtatttcgct
    22141 aaatggcaac cggcgaatgc cccactatcg gtgaatcatc agggattatc ggcggcctcg
    22201 accatttcgt ttaacctgcc gaccggaaaa tcgctctcgg acgccagtgc ggcgatcgat
    22261 cgcgcaatga cccagcttgg ggtgccttcg acggtgcgcg gcagttttgc cggcacggcg
    22321 caggtgttcc aggagacgat gaactcgcag gtgatcctga ttatcgccgc catcgccacg
    22381 gtgtatatcg tgctgggtat cctttacgag agttacgtac atccgctgac gattctctcc
    22441 accctgccct cggcgggcgt tggagcgctg ttggcgctgg agctgttcaa tgccccgttc
    22501 agcctaatcg ccctgatagg gatcatgcta ttaatcggca tcgtgaagaa aaacgccatt
    22561 atgatggtcg attttgcgct tgaagcccaa cggcacggta acctgacgcc gcaggaagct
    22621 attttccagg cctgtctgct gcgttttcgc ccgattatga tgactaccct ggcggcgctg
    22681 tttggtgcgc tgccgctggt attgtcgggc ggcgacggct cggagctgcg gcaacccctg
    22741 gggatcacca ttgtcggcgg actggtaatg agccagctcc ttacgctgta taccacgccg
    22801 gtggtgtatc tctttttcga ccgtctgcgg ctgcgttttt cgcgtaaacc taaacaaacg
    22861 gtaaccgagt aaatgacaga tcttcccgac agcacccgct ggcaattgtg gattgtggct
    22921 ttcggcttct ttatgcagtc gctggacacc accatcgtaa acaccgccct tccctcaatg
    22981 gcgcaaagcc tcggggaaag tccgttgcat atgcacatgg tcattgtctc ttatgtgctg
    23041 accgtggcgg tgatgctgcc cgccagcggc tggctggcgg acaaagtcgg cgtgcgcaat
    23101 attttcttta ccgccatcgt gctgtttact ctcggttcac tgttttgcgc gctttccggc
    23161 acgctgaacg aactgttgct ggcacgcgcg ttacagggcg ttggcggcgc gatgatggtg
    23221 ccggtcggca gattgacggt gatgaaaatc gtaccgcgcg agcaatatat ggcggcgatg
    23281 acctttgtca cgttacccgg tcaggtcggt ccgctgctcg gtccggcgct cggcggtctg
    23341 ctggtggagt acgcgtcgtg gcactggatc tttttgatca acattccggt ggggattatc
    23401 ggtgcgatcg ccacattgat gttaatgccg aactatacca tgcagacgcg gcgcttcgat
    23461 ctctccggct ttttattgct ggcggttggc atggcggtat taaccctggc gctggacggc
    23521 agtaaaggta caggtttatc gccgctgacg attgctggcc tggtcgcagt tggcgtggta
    23581 gcactggtgc tttatttgct gcacgccaga aataacaacc gcgctctttt cagtctgaaa
    23641 ctgttccgca ctcgtacctt ttcgctgggc ctggcgggga gctttgccgg acgtattggc
    23701 agtggcatgt tgccctttat gacaccggtt ttcctgcaaa ttggcctcgg tttctcgccg
    23761 tttcatgccg gactgatgat gatcccgatg gtgcttggca gcatgggaat gaagcgaatt
    23821 gttgtacagg tggtgaattg ctttggttat cgtcgggtac tggtagcgac cacgctgggt
    23881 ctgtcgctgg tcaccctgtt gtttatgact accgctctgc tgggctggta ctacgttttg
    23941 ccgttcgtcc tgtttttaca agggatggtc aactcgacgc gtttctcctc catgaacacc
    24001 ctgacgctga aagatctccc ggacaatctg gcgagcagcg gaaacagcct gctgtcgatg
    24061 attatgcaat tgtcgatgag tatcggcgtc accatcgccg ggctgttgct gggacttttt
    24121 ggttcgcagc atgtcagcgt cgacagcggc accacacaaa ccgtctttat gtacacctgg
    24181 cttagcatgg cgttgatcat cgcccttccg gcgttcatct ttgccagagt gccgaacgat
    24241 acgcatcaaa atgtagctat ttcgcggcga aaaaggagcg cgcaatgaag ttctggcgac
    24301 ccggtattac cggcaaactg tttctggcga ttttcgccac ctgcattgtc ttgctgatca
    24361 gtatgcactg ggcggtgcgt atcagttttg agcgtggctt tattgattac atcaagcatg
    24421 gtaatgagca gcggctgcaa atgctcggcg atgccctggg cgagcagtat gcgcagcatg
    24481 gcaactggcg cttcctgcgc aacaatgatc gctttgtctt tcagatcctg cgttcatttg
    24541 aacacgataa ttcggaagat aaacccggcc cgggtatgcc accgcacggc tggcgtaccc
    24601 agttctgggt ggttgatcaa aacaacaaag tgctggttgg tccgcgagcg ccgattccac
    24661 ctgacggtac acggcgaccc attctggtca acggtgcgga agttggcgcg gtgatcgcct
    24721 cccccgttga gcggttaacg cgcaatactg atatcaattt cgataaacaa cagcggcaaa
    24781 ccagctggtt gattgtcgct ctggcaacgt tactcgcggc gctcgccacc tttctgctgg
    24841 cgcgcggttt actggcaccg gtaaaacgac ttgtcgatgg cacgcacaaa ctggcggcgg
    24901 gcgatttcac tacccgcgta acgcccacca gtgaagatga actgggcaaa ctggcgcaag
    24961 acttcaacca gcttgccagc acactggaga aaaaccagca aatgcggcgc gattttatgg
    25021 ccgatatttc tcacgaactg cgtacaccat tagcggtgct gcgcggtgaa ctggaagcca
    25081 ttcaggatgg cgtgcgtaaa ttcacgccgg agacggtggc gtctttacag gcggaggtcg
    25141 gtacactgac caaactggtt gacgatctcc atcagttgtc gatgtctgat gaaggcgctc
    25201 tcgcctatca aaaagcaccg gtagatttga tcccactgct ggaagtggcg ggcggcgcat
    25261 ttcgcgaacg attcgccagt cgtggcctga aactgcaatt ttccctgcca gacagtatta
    25321 ccgtatttgg cgatcgcgac cgtttaatgc agttattcaa taacttactg gaaaacagcc
    25381 tgcgctacac tgacagcggc ggcagcctgc aaatctctgc cgggcagcgc gacaaaacgg
    25441 tgcgcctgac ctttgccgac agtgcgccag gtgtcagtga cgatcagcta caaaaattgt
    25501 ttgaacgttt ttatcgcacc gaaggttccc gcaaccgtgc cagcggcggt tccgggctgg
    25561 ggctggcgat ttgcctgaac attgttgaag cacataatgg tcgcattatt gctgcccatt
    25621 cgccttttgg cggggtaagc attacagtag agttaccgct ggaacgggat ttacagagag
    25681 aagtatgacc gagttaccaa tcgacgaaaa cacaccgcgt attttgatcg tggaagatga
    25741 accgaagctg gggcagttgc tcattgatta tctgcgtgct gcgagctatg cgccgacgct
    25801 tatcagccac ggcgatcagg tactgccgta tgtgcgccag acaccaccgg atctgatcct
    25861 gttagatctg atgctccctg gcaccgatgg cctgacgctg tgccgggaaa ttcgtcgttt
    25921 ttctgacatt ccgatcgtga tggtgacggc aaaaatcgaa gagatcgatc gcctgctggg
    25981 gctggagatt ggcgcagatg attatatctg taagccgtac agcccacggg aagtggtagc
    26041 gcgcgtcaaa accattttgc gccgttgcaa accgcagcgc gaattgcagc aacaggatgc
    26101 tgaaagcccg ttgattatcg acgaaggtcg ttttcaggct tcatggcgcg gtaaaatgct
    26161 tgacctgacg cctgcggaat ttcgtctgct gaaaacgcta tctcatgaac caggaaaagt
    26221 gttctcccgc gagcatttgc tcaatcatct ttatgacgac taccgcgtag taaccgaccg
    26281 caccatcgac agccacatta aaaacctgcg ccgcaagctg gaatctctcg acgccgaaca
    26341 gtcatttatc cgcgccgttt acggcgtcgg ttaccgctgg gaagccgacg cctgccgcat
    26401 cgtttagttt tagcgacatt attttgttag ccggagatgg cgttcagacc cgatccggca
    26461 acattatccc acgcatggtc agcaaactga cattatctct ccgccctggc aggcatcaga
    26521 aaatcgctca tactttaatc ggtaaacagc acctttagat gctgttttcg atacacaatt
    26581 tcaatcaagg agtcattatg gctggttggt ttgaactcat taagagcagt gataatcagt
    26641 tccggtttgt gctaaaagcg ggcaatggtg agactatcct caccagcgag ctgtatacct
    26701 caaaagcctc tgcggaaaag ggcatcgcgt cggtgcgtag caacagcccg caagaagaac
    26761 gctatgagaa aaaaacggca agtaacggca aattctattt caatctgaaa gccgctaatc
    26821 atcaaattat cggctccagc cagatgtacg ccaccgcgca atctcgtgaa accggaattg
    26881 cctccgttaa agtcaatggc acaagccaga cggtgaaaga caatacgtaa ttacgatgcc
    26941 gggcgtgggt tgttggcgtc cggctttata tccaaataac gcttagttat taataattaa
    27001 gagtaaccgc cgccattaaa atagtgggca ctgacgagca ctcgttgtgc tttctttgtt
    27061 taccatccca tacttatcct cctgaacatg ttcatgtgcc aaaatatctt ctaatacttt
    27121 cgtgacgcta tccggtttat gacatacact gatgtaagat ttttacttac aggcgggcgt
    27181 aaaatggcca gaacgatgac ggttgatctc ggcgatgagt tgcgtgaatt tatcgagtcc
    27241 ttaattgaat ccggcgacta tcgcacccaa agcgaagtga tccgtgagtc actgcgatta
    27301 cttcgagaaa agcaagcaga gtctcgcctg caagcgttac gtgacatgct cgcagaaggg
    27361 ttgagtagtg gtgaagctca gccgtgggaa aaggacgcgt tcttacggaa ggtgaaagca
    27421 gggataagga aataatgtgc gtattattaa actcatgcca aaagccaatg aggatttaga
    27481 aggcatctgg tattacagct atcaccattt cggcgaacca caagccgaca gatatgtgga
    27541 acatctttca gacgttctcc agattctgag taacaataat ataggtacgc ccagacctga
    27601 attaggcgaa ggtatttttg ttcttccttt tgaacgccac gtcatttatt tcctgcaatc
    27661 accaggggaa attatcgtca ttcgtatcct taaccaaaac caggacgcca ccagacattt
    27721 acactggagc taaacccgcc gattgcgcta caatgcccgc ccttaaagtg ggggcactcc
    27781 cctaaccgct tcatcaggtg aagcggatct gacctgtcat cagaacgaga gaattatgtt
    27841 taaaccggaa ctcctttccc cggcgggaac gctgaaaaat atgcgttacg ctttcgctta
    27901 tggcgcagat gctgtttatg cgggccagcc gcgttattcc ctgcgtgtgc gcaacaacga
    27961 attcaaccac gaaaatcttc agctcggcat caatgaagcc cacgcgctgg ggaaaaagtt
    28021 ttatgtcgtg gtcaacattg caccgcacaa cgccaagctg aaaaccttta tccgtgacct
    28081 gaaaccggtg gtggaaatgg ggccggatgc gctgattatg tccgatccag ggctgattat
    28141 gctggtgcgt gagcacttcc ctgaaatgcc gatccacctt tcggtgcagg ctaacgccgt
    28201 gaactgggcg acggtgaaat tctggcagca aatgggcctg acccgcgtga tcctctctcg
    28261 cgagctgtcg ctggaagaga ttgaagagat ccgcaatcag gtgccggata tggagatcga
    28321 gatcttcgtt cacggcgcgc tgtgcatggc ctactccggt cgctgcctgc tctctggcta
    28381 tatcaacaag cgcgacccga accagggcac ctgcaccaac gcctgccgct gggagtacaa
    28441 cgtccaggaa gggaaagaag atgatgttgg caacatcgta cacaagtacg agccgattcc
    28501 ggtgcaaaat gttgagccga cgctgggtat cggcgcacca accgacaaag tgtttatgat
    28561 cgaagaggcc cagcgtccgg gcgagtatat gaccgcgttt gaagatgagc acggcactta
    28621 catcatgaac tcgaaagatc tgcgcgccat cgcccatgta gaacgcctga ccaaaatggg
    28681 cgtgcattcg ctgaaaatcg aaggtcgtac caaatctttc tactattgtg cacgcaccgc
    28741 acaggtttac cgcaaagcta tcgatgacgc cgctgcggga aaaccgttcg ataccagcct
    28801 gctggaaact ctggaaggtc tggcgcatcg tggctatacc gaaggtttcc tgcgtcgtca
    28861 tactcacgac gattatcaga actacgaata cggttattca gtttctgacc gccagcagtt
    28921 tgttggtgag tttaccggtg agcgcaaggg ggacctcgcg gcggtagcgg tgaaaaataa
    28981 attctccgtt ggcgacagcc ttgagctgat gacgccgcaa ggcaacatta attttaccct
    29041 tgagcacatg gaaaacgcca aaggtgaagc tatgccgata gcaccaggcg atggttatac
    29101 tgtgtggctc ccggtcccgc aggatcttga gctcaattac gcgctgctga tgcgtaattt
    29161 ctccggggaa accacgcgta atccccacgg taagtgatta atttcgatta tttttcccgg
    29221 atggaaaatt cttagaaacc gatcacatac agctgcattt attaaggtta tcatccgttt
    29281 cgctgaaaaa cataacccat aaaatgctag ctgtaccagg aaccacctcc ttagcctgtg
    29341 taatctccct tacacgggct tattttttat gcataagccc tatccctggt caccgtcttc
    29401 cattgaccac atcgatagaa tctcccttca tagcacgatg cctttcacgt aacggcatcg
    29461 tgctcgcaca ggttccggct aagcacaacc agaacgcgca tgtttgacgc ttaccaaaaa
    29521 atattctcac tctccacatt tgaatgtcag acgagcgacg ccatgtaatc ctgcaccttc
    29581 tgtcttcagg tcaactatct gcattttttt gccctgagta acacagaaat gggctgcatc
    29641 attttttact atattttctg caccagatat tctgcccctg gctaaagaag cttcggcttc
    29701 ggtgtagtat tggttatcga gtttacgctg aatattactt ttatatgcaa gaccaaattt
    29761 accgatactt gtctcatcat tatgcacagc acaaccagac acaataaaaa tactaattaa
    29821 tgatatagca gctatctttt tcatctcacc ttcccccatt aaataccaac gacactctct
    29881 agtg
//
LOCUS       DAZGVQ010000050        29825 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000050 DAZGVQ010000000
VERSION     DAZGVQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.050"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            50..1339
                     /gene="uraA"
                     /locus_tag="ACHLAG_002113"
     CDS             50..1339
                     /gene="uraA"
                     /locus_tag="ACHLAG_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFP0655348.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            1434..2135
                     /gene="hda"
                     /locus_tag="ACHLAG_002114"
     CDS             1434..2135
                     /gene="hda"
                     /locus_tag="ACHLAG_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFP0655349.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(2273..2632)
                     /gene="arsC"
                     /locus_tag="ACHLAG_002115"
     CDS             complement(2273..2632)
                     /gene="arsC"
                     /locus_tag="ACHLAG_002115"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFP0655350.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2653..4116)
                     /gene="bepA"
                     /locus_tag="ACHLAG_002116"
     CDS             complement(2653..4116)
                     /gene="bepA"
                     /locus_tag="ACHLAG_002116"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFP0655351.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            4329..5390
                     /gene="yfgO"
                     /locus_tag="ACHLAG_002117"
     CDS             4329..5390
                     /gene="yfgO"
                     /locus_tag="ACHLAG_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP0655352.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(5428..6276)
                     /gene="focB"
                     /locus_tag="ACHLAG_002118"
     CDS             complement(5428..6276)
                     /gene="focB"
                     /locus_tag="ACHLAG_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFP0655353.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQEP"
     gene            complement(6298..8310)
                     /gene="hyfR"
                     /locus_tag="ACHLAG_002119"
     CDS             complement(6298..8310)
                     /gene="hyfR"
                     /locus_tag="ACHLAG_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFP0655354.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVTLRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(8340..8753)
                     /gene="hyfJ"
                     /locus_tag="ACHLAG_002120"
     CDS             complement(8340..8753)
                     /gene="hyfJ"
                     /locus_tag="ACHLAG_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFP0655355.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(8746..9504)
                     /gene="hyfI"
                     /locus_tag="ACHLAG_002121"
     CDS             complement(8746..9504)
                     /gene="hyfI"
                     /locus_tag="ACHLAG_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HFP0655356.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(9501..10040)
                     /gene="hyfH"
                     /locus_tag="ACHLAG_002122"
     CDS             complement(9501..10040)
                     /gene="hyfH"
                     /locus_tag="ACHLAG_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFP0655357.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPECKQ
                     RATLINDDTDVPLVAKEQL"
     gene            complement(10050..11765)
                     /gene="hyfG"
                     /locus_tag="ACHLAG_002123"
     CDS             complement(10050..11765)
                     /gene="hyfG"
                     /locus_tag="ACHLAG_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFP0655358.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVWEKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVHEMRADVSELVEMLLAMPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(11755..13335)
                     /gene="hyfF"
                     /locus_tag="ACHLAG_002124"
     CDS             complement(11755..13335)
                     /gene="hyfF"
                     /locus_tag="ACHLAG_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFP0655359.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(13340..13990)
                     /gene="hyfE"
                     /locus_tag="ACHLAG_002125"
     CDS             complement(13340..13990)
                     /gene="hyfE"
                     /locus_tag="ACHLAG_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFP0655360.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(14002..15439)
                     /gene="hyfD"
                     /locus_tag="ACHLAG_002126"
                     /pseudo
     CDS             complement(14002..15439)
                     /gene="hyfD"
                     /locus_tag="ACHLAG_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(15456..16403)
                     /gene="hyfC"
                     /locus_tag="ACHLAG_002127"
     CDS             complement(15456..16403)
                     /gene="hyfC"
                     /locus_tag="ACHLAG_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFP0655361.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(16414..18431)
                     /gene="hyfB"
                     /locus_tag="ACHLAG_002128"
                     /pseudo
     CDS             complement(16414..18431)
                     /gene="hyfB"
                     /locus_tag="ACHLAG_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(18431..19048)
                     /gene="hyfA"
                     /locus_tag="ACHLAG_002129"
     CDS             complement(18431..19048)
                     /gene="hyfA"
                     /locus_tag="ACHLAG_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFP0655362.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVLPQHALLRREPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(19301..19771)
                     /gene="bcp"
                     /locus_tag="ACHLAG_002130"
     CDS             complement(19301..19771)
                     /gene="bcp"
                     /locus_tag="ACHLAG_002130"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFP0655363.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(19771..20343)
                     /gene="gcvR"
                     /locus_tag="ACHLAG_002131"
     CDS             complement(19771..20343)
                     /gene="gcvR"
                     /locus_tag="ACHLAG_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFP0655364.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            20489..21367
                     /gene="dapA"
                     /locus_tag="ACHLAG_002132"
     CDS             20489..21367
                     /gene="dapA"
                     /locus_tag="ACHLAG_002132"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFP0655365.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            21384..22418
                     /gene="bamC"
                     /locus_tag="ACHLAG_002133"
     CDS             21384..22418
                     /gene="bamC"
                     /locus_tag="ACHLAG_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFP0655366.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VSVFQAAFSK"
     gene            22631..23344
                     /gene="purC"
                     /locus_tag="ACHLAG_002134"
     CDS             22631..23344
                     /gene="purC"
                     /locus_tag="ACHLAG_002134"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFP0655367.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            23498..23725
                     /gene="vapB"
                     /locus_tag="ACHLAG_002135"
     CDS             23498..23725
                     /gene="vapB"
                     /locus_tag="ACHLAG_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFP0655368.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            23725..24123
                     /locus_tag="ACHLAG_002136"
     CDS             23725..24123
                     /locus_tag="ACHLAG_002136"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFP0655369.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            24270..25133
                     /gene="ypfJ"
                     /locus_tag="ACHLAG_002137"
     CDS             24270..25133
                     /gene="ypfJ"
                     /locus_tag="ACHLAG_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFP0655370.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            25148..27163
                     /gene="tmcA"
                     /locus_tag="ACHLAG_002138"
     CDS             25148..27163
                     /gene="tmcA"
                     /locus_tag="ACHLAG_002138"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFP0655371.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLAGRKMLLVRQREEVAHALFALNDVRTERLRDRITQWQFFH"
     gene            27237..27935
                     /gene="ypfH"
                     /locus_tag="ACHLAG_002139"
     CDS             27237..27935
                     /gene="ypfH"
                     /locus_tag="ACHLAG_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP0655372.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(28045..28245)
                     /gene="ypfN"
                     /locus_tag="ACHLAG_002140"
     CDS             complement(28045..28245)
                     /gene="ypfN"
                     /locus_tag="ACHLAG_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFP0655373.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(28273..29400)
                     /gene="dapE"
                     /locus_tag="ACHLAG_002141"
     CDS             complement(28273..29400)
                     /gene="dapE"
                     /locus_tag="ACHLAG_002141"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFP0655374.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(29404..29760)
                     /gene="yffB"
                     /locus_tag="ACHLAG_002142"
     CDS             complement(29404..29760)
                     /gene="yffB"
                     /locus_tag="ACHLAG_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFP0655375.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         7215 a         7774 c         8006 g         6830 t
ORIGIN      
        1 cttttttttg agtaaagcgc ctataacaca taatacagag gataatacta tgacgcgccg
       61 tgctatcggg gtgagtgaaa gaccgccact tttacagaca atcccgctta gtttgcaaca
      121 tttgttcgcc atgtttggtg caaccgtcct ggtgcccgtc ttatttcata ttaacccggc
      181 gactgtactg ttatttaacg gtattggaac gctgctgtat ctcttcatct gtaaagggaa
      241 aattccggct tatcttggtt ccagctttgc ctttatttca ccggtattgt tactgttgcc
      301 gttagggtat gaagtcgcgc tgggcggctt tattatgtgc ggcgtgctgt tctgcctggt
      361 ttcttttatc gtgaagaaag cggggaccgg ctggctggac gtgctgtttc cacctgcggc
      421 aatgggcgca atcgttgccg tcatcggtct ggagctggcg ggcgtagctg ccggtatggc
      481 gggtttactc ccggctgaag ggcaaacgcc agactccaaa accatcatca tctctattac
      541 caccctggcg gtcacggttt taggttccgt gctgtttcgt ggtttcctgg caattatccc
      601 gattttaatt ggcgtgctgg tgggctacgc gctctctttc gcaatgggaa ttgtcgatac
      661 cacgccgatt attaatgctc actggtttgc gctgccaacc ctctatacgc cgcgcttcga
      721 gtggtttgcc attctgacta ttctgccagc ggcgttagtg gttattgccg aacacgtagg
      781 gcacctggta gtaacggcta atatcgtcaa aaaagatctg ctgcgcgatc caggtctgca
      841 ccgttcgatg tttgctaatg gcttgtcgac cgtgatctcc ggcttctttg gctctacgcc
      901 aaataccact tacggagaaa acattggcgt gatggcgatc acccgtgttt acagtacctg
      961 ggttatcggc ggggcggcga ttttcgctat cctgctttcc tgcgtcggta aactggctgc
     1021 cgctatccag atgatcccat tgccggtgat gggcggcgtt tcgctgctgc tttatggtgt
     1081 catcggtgct tccggtattc gtgttttgat cgaatcgaaa gtggattaca acaaagcaca
     1141 gaacctgatc ctgacttccg tgattttgat catcggcgtc agtggcgcga aggtaaacat
     1201 cggcgcggcg gagctgaaag gtatggcgct ggcgaccatc gtcggtatcg gcttaagttt
     1261 gattttcaaa ctgatcagtg tgctgcgccc tgaagaagtg gtgctggacg cagaagacgc
     1321 ggatataaca gacaaataat gtaccgaccg ggcagtgttg ctgcccggtt caaacatcat
     1381 gggattctgt ggtaaacttc tcgcgatttt gtgaaatcct ggttgaggta tctctgaaca
     1441 caccggcaca gctctctttg ccactttatc ttcctgacga cgaaaccttt gcaagtttct
     1501 ggccggggga taactcctct ttactggccg cgctgcaaaa cgtgctgcgt caggaacata
     1561 gcggttacat ctatctctgg gcacgcgaag gcgcggggcg cagccatctg ctgcacgcgg
     1621 cttgcgcgga attgtcgcag cgtggcgatg cggtgggcta tgtcccgctg gataaacgca
     1681 cctggtttgt tccggaagtg ctcgacggta tggagcattt gtcactggtc tgtatcgaca
     1741 acattgagtg tattgccggc gatgagttgt gggagatggc gattttcgat ctctacaatc
     1801 gaattctgga atcgggcaaa acacggttgt tgatcaccgg cgatcgtcca ccgcggcagt
     1861 tgaatctggg attaccggat ctcgcgtcgc gactcgactg ggggcagatc tacaaattgc
     1921 agccactttc tgatgaagat aagttgcagg cgctacagtt acgcgcgcgt ttgcgtggtt
     1981 ttgaactgcc ggaagatgtg gggcgtttct tgctgaagcg gctcgacaga gaaatgcgca
     2041 cgctatttat gacgttggat cagttggatc gtgcgtcgat taccgcgcaa cgtaagctga
     2101 ccattccgtt tgtgaaagaa attctgaagt tgtagataag gtgtttattg tcggatgcgg
     2161 cgcgaacgcc ttatccgaac tacgcgaagt taatccatag tattcgtagg ccggataaga
     2221 cgtggcaagc gtcgcatccg gcaatgtgct tcaactagcg atggagcaca tcttaaccca
     2281 cgatttccag tacctgctcc ggtggacgac caatccgcgc tttgccattc gcgaccacaa
     2341 tcgggcgctc catcagcttc ggattatcca ccattgcctg aatcagcgcc tcttcgctaa
     2401 gcgaactgtc agccaggttc agttctttat aaagatcctc tttctggcgc atcaattcac
     2461 gggcgctatt catccccagc attttcagca aatcgcgcag cgttgccgca tcggcgggtg
     2521 tctcaaggta gagtaccact tctggctcca cgccgttttc tttcagcagg ttcagcgttt
     2581 cccggctctt tgaacagcgt gggttatggt agatttttac ctgtttggtc attgttcttc
     2641 ctttaatgcg aattacatct tggtataagg cttaaagcgt tcctgcagct ggcgcaactg
     2701 gtcgatgcgc gcatcgtaac gcgcttgttg caggctgcct aatttcacct gcgaactggc
     2761 gctactcaac agcgaaatgg cctgatcgag tcgtccggcg agcgcataac cttctgctcg
     2821 cgcagccagc tcctgatcgc ggttatttag cgcggcttcc gcctgtgcca gtaaatccca
     2881 gccgttgctg tcatctttat tattaaaggt gtagcgattc agaatattgg ccgcttcttg
     2941 tggttgaccg ccttgtagat aagcgttcgc caggttgagc tgcaacaccg gattggtgcg
     3001 caaatcacgg gcatttttca ggcgattgat tgcctcattg gctttgtttt gcccaagatc
     3061 gatatcggta gccagatcga gataccatgc gttgccaggt tctgccgcca gtaacggttg
     3121 cagcgtttta cgcgcctcgt cgtatttatt ggcttccatc gcctgtaaag cacgaccata
     3181 ttgcgccgct cgttgctgac gaacgtttcc tttcgcccat tcatccagca aatcactggt
     3241 gagctggtta cgtccggaat tatacatccc cagtgtgcgc gctttcgcca gatagaaatc
     3301 ttccgacgac tgcaccacca tcggtcgcat ctgattagca cggttgcggg catctgccag
     3361 acgactttcc ggcaacgggt gagtcaataa aatttccggc gggcgcgagg agtaacgcgc
     3421 ctgatcgagt aatttttcga ggaaggttgg catcgcctgc ggatcgaatc ccgagcgttg
     3481 cagcacctga ataccaatgc gatccgcttc ctgttcattt tgctgggtga agctgatcat
     3541 cccctggcgc gttcccgcca gtgtaccggt cagcgctgcc atccccgcct gcggactggc
     3601 catcgccagt aaaatagaac ctaacgcgcc gacccaggtc agcggcgcgc tgcgctgttg
     3661 atcttccatc gctcgcgcca ggtgacgttg ggtgacgtgg gagatttcat gcgccattac
     3721 tgaagccagt tggctttcgt tatcggaata acggaacagg gcagagtgca gtaccacgtt
     3781 gccgccaaag aaagcaaagg cgttaatttc gtcgttgttg atcagaaaaa aatgaaacgg
     3841 tgtcttaacc gaattggcat gcgaaaccag acgcattcct agtgaattaa tgtactgcgt
     3901 taatagtgga tcattaatta acggcgcgct gccgcgtagc tggcggacat aatagtcgcc
     3961 catctgcatt tcctgaccaa tggaaagcgt gcttcctgcg gaggttccca tatccggcaa
     4021 ggtgtctgca ctatcggcaa acgccggggc tacctgacca atggtcatgg cagcaatgag
     4081 ggttgcaacc aggttttttt tcaactgcct gaacataacc tctatcctgt atttctggag
     4141 atagtcattt gaccgatact gtgaaatatc gttcccaccg cgccgccttt gagtgtaacg
     4201 gcgaagatta gtccactcaa atgacttttc agtttcctga atccgtcata aaaagcgaag
     4261 tcttttttgt gacatttcga tacaattgcg gatcgcaatc ccgctcttga ggttcaggga
     4321 aggtctttat gctcgaaatg ttgatgcaat ggtatcgccg ccgttttagc gacccggaag
     4381 cgattgcctt gctggttatt ttagttgccg gatttggcat tatctttttc tttagtggcc
     4441 tgcttgctcc gctgttggtg gctattgtgc tggcctattt gttggaatgg ccaaccgtgc
     4501 gcctgcaatc tattggctgc tcccgccgct gggcgacgtc gattgtattg gtggttttcg
     4561 tcggtatatt gctattgatg gcgttcgtgg tactgcctat cgcctggcag cagggcatct
     4621 acttaattcg cgatatgccg gggatgctca ataagctttc tgactttgcc gccacgttgc
     4681 cgcgccgcta tccggcgttg atggatgcgg gcattattga tgcaatggcc gaaaatatgc
     4741 gcagtcggat gctgaccatg ggcgattcgg tggtgaaaat ttccctcgcc tcgctggtcg
     4801 gtttgctgac cattgccgtc tatctggtgc tggtaccatt gatggtcttc ttcctgctga
     4861 aagacaaaga gcagatgctg aacgccgttc gccgggtgct gccgcgtaac cgtggactgg
     4921 caggacaggt gtggaaggag atgaatcaac aaatcaccaa ctatatccgc ggcaaagtgc
     4981 tggagatgat cgtggtgggg attgccacct ggctgggatt cttactcttc gggctgaact
     5041 attcgctgct gctggcggtg ctggtcggct tctcggtact tattccgtac attggtgcat
     5101 ttgtggtgac cattccggtg gttggcgtgg cgctattcca gtttggtgca ggcacggaat
     5161 tctggagctg cttcgcggtg tatctgatta ttcaggcgct ggacggcaac ctgttagtac
     5221 cggtgttgtt ctccgaagcg gttaacctgc atccgctggt gattatttta tcggtggtga
     5281 tcttcggtgg tttgtgggga ttctggggcg tattcttcgc cattccgttg gcgacgctga
     5341 tcaaagccgt gatccacgcc tggcccgatg ggcaaatcgc gcaagaataa tcttcagccc
     5401 gacgaactgt ttcgccgggc tgatttttca gggttcctga cgtaaataaa tagcccgata
     5461 acaaatactc accagcactg caccgccgat aatattaccc agcatcaccg ggagcagatt
     5521 ggcggtaata aaatggctga ccgtcagtgc cggaaaattg tctgcgctac tgtgcgccag
     5581 ttgccagaag gaggtagggg cgaaatggcg aatggcaatg gcgaacggaa tcacaaacaa
     5641 attggcgata cagtgctcaa agccactggc gacaaacagg gtgatgggca aaatcatggc
     5701 gacgattttg tcgcataacg aacgcccgca ataactcatc cacagcgcca ggcaaaccat
     5761 cagattgcac ataatgccga ggctgacaga ttcagtaaat gtatgatgca ttttgccctc
     5821 ggcgcagtgt aaaaccgcga ctccccacat cgcgttttca ctcatcacca gcccggaaaa
     5881 ccagattaac aaactgaaac aggcaatacc tgccagatta ccgcaggcca ccagaagtgc
     5941 gttaatcagc caagttcgcc aactaataac gccccgactt tttgccatta ccgtcattac
     6001 cgacgaggtg aacaggttgg tgccgcaaac cgccagcaaa ataaagccaa gtgtaaagca
     6061 aaggccgccc accagatggg ttaatgcctg tgaagacggg gcatcggcga taactgaaag
     6121 gtaaaaagta aatccgatcg ccataaacac gccagcggac atcgccatta aaaagaccga
     6181 cactttactg cgggcaattt tatgggcggc aatccgttca gcgattgccg cttttctggc
     6241 gctcaactgc aagtcgaaag agagtttgtt tctcatgcat actcctgcaa aagcagatta
     6301 caacacctcg cgaaccgaga ttcccagacg ctgcattcgt gacagcagcg tggtgcgctt
     6361 catccctaat cgtgtcgccg ctccacgggg cccggcaaca atgccattgg tttctcgcaa
     6421 tacctgaaca atgcgctgac gttcttcttc gtcattctct ggcgtcgtcg ggtgcagcaa
     6481 ctgcgccatt gaactgcgaa gcgctgaatc ttcatttagc gtcggcagta aacggctttg
     6541 tcggacatta agatgtaaat tcagactgtt accgcgagtc agcagtaccg ctcgctcaat
     6601 cacgttttcc agctcgcgca cgttgcccgg ccagtcccac gacatcaact ggcgtagcgc
     6661 ctcggtcggg atggcgtcaa ttgaacgatt catatggcgc gccatttttt gcgtgaaatg
     6721 ctttgctaaa agagggatat cttccggacg gtcgcgcagc ggcggcaatt ccagtgggaa
     6781 gacattcagg cgataaaaga gatcgctgcg aaactggcga tcttcaacca tttgccacaa
     6841 atcacggttg gtggcggcaa tgactctgac atttaccggg atcgttctac tcccgccgag
     6901 acgctcaatc tcccgttcct gcaatacgcg cagcagttta ggctgaagtt ctaacggcag
     6961 atcgccaatt tcatcgagaa acaacgtgcc gccatcggca atttcaaaac gaccacgatg
     7021 tgtattaatc gcaccagtaa acgccccttt gtcatgaccg aataactcac tttccagaag
     7081 actggcgggg atggcagcgc agttgatttt gaccagcggc ttgtcgcgtc gcgggctaag
     7141 ttgatggatc gctctggcga tcacctcttt gccggttccg gtttcaccgc aaatcaacac
     7201 cgtactgtcg ctcttcgcca caatatctac ctgctggagc aggtcttcca ttgcctggct
     7261 ttgatagata atgtcaccga cgcccagatt cgaaaggagc tgctcgctaa gctggtggtt
     7321 ttcttgctgc aaactttcct gcaaatcggt catgctgcgc caggcatcgg cattgccaac
     7381 ggcaatagcg atgcgatcgg ctatgtgttg tagtagctgg cagttttcct cactaaagag
     7441 agtggaagag gtatgcgcca gcaacaatgc acccggtgta tggttgccaa aggtaagcgg
     7501 tatcaggagc gcagattcgc agccgttaga tgcaagtaag agtaataacg ggtcgcgttg
     7561 ccagagaaac agatcatctg cacgatgcgt caaggtcggt tgtcgggttt gtagcgtttg
     7621 tgtcagcaat acactgtcgc caggcatatt gcgtggcaga cacgcacaat gtgaggcaga
     7681 aagatcgctg caccacaggc tgaacttctc gttctttcga tgatcgccca gtaccatact
     7741 gactgaagcc agaccgaaaa aatgatggat ctcacgggcg acgtcagcga tcagatcgtc
     7801 gagatcaaga tgtgacagca ctgcattagt gatatccacc agtacccgat gatgatcgcg
     7861 atctttctcc agcaaacgca gcgtcacctg ctgttcttca tcattgagcc atgattgcag
     7921 aaccggggtg ataatgcgtg ccagccagtt aaacagtgtg caatcctggg gagtaaaacg
     7981 gcagccgtgg gtacgaataa agcgtagctc cgccattcca ctactgccga gaaggacggc
     8041 gtgacgcgaa taatcgacca tctcgccctg atactctgtt gttgccgtca catcgctgcc
     8101 atcatcatgg tagcggtaat agaagttgct tttgagaaca tgcagttcaa taagttgtac
     8161 cgggctgaac ggcggcggca gcgggatgaa ggcgcgtaat aaagacgcct taccgtgttt
     8221 ctgcgctaac cgctccgcga gcattccgtt taccgcttca attgttattc cttgcggcgg
     8281 ggcaaacatc gcctcgtctg acatagccat caacagtcct tattaatgag aaatccttct
     8341 cacgcaattt ttctggccat cagatagatg gccggttcct gcacaatttc atccaacaga
     8401 tgaatgaatt gaatgctacg cgcctgccac acagacgtcg cgtcatccgc cagtggcgcg
     8461 aatgcccggg tcaacagggc ggtgtggctg gcgtcaataa caatctcacc aaaagtcatc
     8521 acccccagca tcttacgtcg ggcttgctcc tcttcgacct gggcaagcca atcttcgtat
     8581 tccgtcagtg ggcaggggaa ggcgacattc agacaatcaa tcacgccaac gtgatgaccg
     8641 atagccagcg agtaatacat tacctgagag ctgtgttccg gcatctcgtc gctactggcg
     8701 acaaactttt ttcgcagcgt ccagaaaaca atttccccgc actcttcagt catgtaaccc
     8761 ccggagtacg cgaaagagtt gctgaacgat actattgaga cgtggatcgt cggcatcgcg
     8821 caaccaggtg ttaacccgat ttcctgtagg atcgtcgctt aaatggcgca ggagccgatc
     8881 gcaaatttct cgcccctgac gatagcccgc cagccgccgc gcttctcgct caatggcgat
     8941 acgctgtgat ggcgggatct gtggccacaa cggttgcata gtcaccccgg tgggatcgcg
     9001 ataatccaca gcgtgaatct tctgttgcag caaaccgagc gccacggcga aaccgtgaat
     9061 ggtggcggcc ggtgttggcg ggcagccggg gatccaaaca tcaatgggga caatggtgtc
     9121 gctgccgccc cagacgctgt agagatcgtg gaaaataccg ccgccgacac cgcacgcgcc
     9181 gtaggaaaca cagattttat gatcgggggc agactcatac gcccgaagtg caggcatacg
     9241 catcgcccgg gtgactgcgc cagtaaataa caaaatatcg gcgtgacgcg gtgatgaaac
     9301 aaccttaatg ccaaaacgtt ctgcgtcgaa tactggtgtg atggcagcaa aaatttcgat
     9361 ttcacaggcg ttgcagccgc cgcaatcgac gcgataaacg taagccgagc gacggatatc
     9421 ctgtagcaaa tgctgcttca tcttttgcgt ttgctcgtcc agcgtgatgg gctggctgac
     9481 atattgcgta agcactggac tcatagctgc tccttagcca ccagcggtac atctgtatcg
     9541 tcgttgatca gcgtcgcgcg ttgtttgcac tccgggcaga cgctcgcttg cgcccgcaac
     9601 atttcgcggt tttgtggcgc attttgttgc tgtgccaaca attcagcagc cagtgcgacg
     9661 gttttttgcg gggcaaacgg gcgttcgcaa cggctgcaac gttgtagatg gaacgtcgcg
     9721 cgggtataga gatcggcttt attggtgacg gtcagttcaa agttattggt aagctggatg
     9781 gctctggtcg ggcacacttc ttcacaacgt ccgcagtaaa tacaacgcct cagatagagc
     9841 tgccaggtgc gcgaattttg ctggtcgtcg gtctggatag tcagcgcatt tgccggacaa
     9901 gcacaggcgc cgcaggcaat acattggctg ggcatcaggt ccggtttccc gcgaaagcct
     9961 gggctgacct ccagtggcgc gaagggatat ttcaccgtcg cggttccggc gcgcataata
    10021 gttttcagta acttcagcat cttctgtcct tacttcagcg gcgaacggtt acgatcaatg
    10081 ccgtagcgtt cgatctcttt atacggcacg gtttttgact ggcgcttgcg cacatctacc
    10141 agcgtcacac ggtcggtaca ggagtagcag ggatcaaggc taccgataat cagcggtgcg
    10201 tcagaaacgg tattgccgcg cagcatgtaa cgcaacaccg gccagttggc gtaggtggcg
    10261 gcacgacagc gccagcggaa caatttttgg ttatcaccga gcatgctcca gtgcacgtct
    10321 tcaccgcgtg gcgcttcaac aaagcccagc gcgaatgcgt gaggtttata gctaaagcct
    10381 tcggtcagca gcggggtatc cggcatgttg tcgagggcaa attccagcat tgccagcgaa
    10441 tcaaacgtct ctttgacacg gaccatcacg cgggagaaaa catcgccgcc ggtgaaggta
    10501 aacagtgttt ttggaatatt gccgtagtcg gcgtaggggt gatcaaagcg caaatcacgg
    10561 gcaaaaccac tgccgcggat cagcggccct acagggctat aatcacgggc gatttgtcgg
    10621 tcgagaatgc caatgccctg agtgcgttgt tccatattcg gcatagcaag cagcatctct
    10681 accagctccg acacgtcggc gcgcatctcg tgcaccagtt tcagcgtttg cagacgttgc
    10741 tctttgagaa tatcgcggcg aacaccacca atcagattca gaccgtaggt tttacgcgac
    10801 ccggtcagca attccgccat cgtcatcgac ttttcccaca cgcggaaaaa ttgcataaag
    10861 ccggtatcaa aaccaacaaa atggcaggag aggccaaggt tgagcaaatg actgtgtagc
    10921 cgttcgactt ccagcagaat cgagcgaatg gtatgcgctc gttgcggcac ctcaatcccc
    10981 agtgcatttt caaccgagtt ggtataggcc acactgtggg caaaaccgca aatcccacac
    11041 acgcggtccg ataagaaggt cacttcgttg tagcccatcc gcgtttctgc cagtttctcc
    11101 atgccgcgat ggacataaaa caggcggtaa tcagcatcga caatttgctc gccatccaca
    11161 aacaagcgga agtgacccgg ttcatcggag gtgatatgca gcgggccgac agggatcacc
    11221 cgcgcatcgc tgttgccctc attgataaac gcatacgttt cggaatctgt cgtcggttca
    11281 gggcgcagtc gataatccat cgcatctttg cgcagcggat gcatatcttc cggccagtca
    11341 tcgggcaaca ccaggcgacg ctgatccggc aggccaaccg gaatcagccc gtacatatcg
    11401 cgaatttctc gctcgcccca gaccgcggca gggacgcgcg gtgtgactga cggaaactcc
    11461 cgactgtcgg catcgaccag tgccttcacc acgatccagc atttttcggc cccttccatt
    11521 gaaagggcat aataaaccgc gtaatgaccg ttaagtgtcc gctcgtcgtt gccaaacagg
    11581 actggaagcc agccatcatg ttgataataa agataatgta caacgtcagg cagcagattg
    11641 attttcaccg taatggtgac ctgttcaggc gtttgtcgct cttcatccag caccgcgccg
    11701 gggaactgcg ttttcagggc ggcgagaatc gcttcgccac gatttgacga tgaattaacg
    11761 ttcactgtgt ttctccgaaa cagatgcggt gcctgaaggc aaaaaatcat gccaggtgct
    11821 acgttgtgca ggcaggtcgt gcgtccctga gaggactata gtggaagcgc ccgccaggat
    11881 cctgatgaca ggttgtggaa tatgcgttcc catcgccagc atcatgacca gcagaatcac
    11941 cattggcgag gtggtcaacc agccgagatc accccggtta acggcctgcg gcggtttcgc
    12001 cattaacacc cgcgcagcca tccgtaccag gcccgccagc accagcgtta acagcaataa
    12061 cagcaggacg ataatcagca ggtgattacg tgccagtcct gcggtaacgg tcataaattc
    12121 gctaagaaaa atgttgaagg gcggcatccc tgccagcgcc agcgcaccgc cgccaaacag
    12181 cacggcggta aatggcatga ttttgagcat cccacagacg acgttgagat cgcgcgtgcc
    12241 gtacttgagc agtacattgc cggaaccgca gaacagcagc gtttttgcca gactgtggtt
    12301 taaggtgtgc agcagcgcgg caaaaattcc cagcggcccg ccaatgccta gcgccaccgc
    12361 gaccagcccc atgttctcca cgctggagta cgccagcaga cgcttaatgt cccgctgtac
    12421 cagaatgaaa aatgccgcca cggcaaccga caacatgccg aagatgagca acaaccggtt
    12481 ggggaaatcg ctgccgatgg cctggcagat aatgatgtaa tagcgaatta gcaccaacag
    12541 cgcgcagttc agcaatacgg cggagagcag ggcgctgacc ggactcggtg cttcactgtg
    12601 agcatccggc agccaggcgt gcatgggaaa tagcccggtt ttggtgccaa agccaattag
    12661 cacaaacaca aaggccaaca gcattaatgt tgggtcaagc aaggacgatt gcttaagaac
    12721 ctcgctccag aatatcgcca tttctgcctg cggcataacg ctggcgacgt tggcgtatac
    12781 cagcacggta ccgaacagac caaaagcgac accaacagta caaataatga tgtacttcca
    12841 tgctgcttcc agcgatgaac gctgaccgta aatgcctacc agaaacgccg agcttaaggt
    12901 ggtggcttcg atcgccgccc acatcacaat caggttattg ctggtaacaa ccagcagcat
    12961 ggtaaacaaa aacagatgga agaaaccgta gtaatcgcac agcgtaacgg gggaaagctc
    13021 gccgtgtgcc acttcatgac gcatgtagcc aatcgagtaa acaccggtga gaaagccaat
    13081 cacaccaaga atggcgagga acaaaccgcc cagaccatca atatgaagcc acagtcccgc
    13141 agcgaatatt tctcctgcat cagcggcagt ttggaccacc cagagtgcca gaatcagcag
    13201 cagtgtgatc cctaagctat gtaatacagt caccgtacaa gtcgcagaga gcccccgttt
    13261 ccggcaggca aaacagagca gcgaaaaaag cagcggcgtg agcaggagta aagcgaacat
    13321 cacagaataa ctcatctcat tatcccttca gcgcggtcaa gttgttcacg tccagcgtgc
    13381 cgtgggtacg ccatattttt cttgccagta acaccatcac aatgacggcg aagatggcgt
    13441 cggtagcgat acctatttcc accagttccg gtgctcgcca ggcaagaagc gccagcacca
    13501 gatgggagcc gttttccatc aggcagtaac caaaaatttg ccgcaggata ttgcgctggc
    13561 tgacaatgca cagcaggcca agcagaaaat gacctaacgc taccgccagc gccggtttca
    13621 gcccggtagc catcggtagc ttcacgggct gaacgacaaa tgcgcaaagc aggacaatta
    13681 acgccgcgag cagtgccatc attgccggac cgaataacgc tttttccggg atgttctggg
    13741 gaatatttcg tgcagcgtaa gtcatgatta acggtaccag cagcactttg gtgataaagg
    13801 cgctggcgga ccagatcagc agttgctctg cggcgaacag gcacgagaga gtggcgaaaa
    13861 tagacaccag caccagcgac tggcaggcgt aaaatccgca tgacaggcga tagcttttga
    13921 cgctaatcac aaacagcgat gtcagcatca tcagtcccgc cagattattt acgatcatag
    13981 aaccggtcat tcatcatctc cttactgcaa ccaggtcgcg gcgattacgc tggaaatcag
    14041 cgacatcaca atcagtacaa tcaacaccag gcgcattgac cctggcagcg gtgcggcatc
    14101 ggcgacggcc tcgctcggtt tgccaggcac aacgcgacca aaccagcgaa taaaccaggc
    14161 gaaactggcg accgattcaa tcatcagcag aatcatggcg ggcagcagga tccagtactc
    14221 cactgacaac gcaaaaccgg cagcaaacag cgggaattta ctgaagaagc cgttgaacgg
    14281 cggcacgccg gtaatcgcca gcgctgccac gcagaaaccc acgcctggca acggcagggt
    14341 gtgcaatacg ccacgcagac gcggcaacaa gcgcgtgccg cagctgtaac tcagcgcacc
    14401 tgctacaagg aaaaacaggc ttttagcgaa cgcgtggttg acgatgtagg cgatgctacc
    14461 ctccagcgcc agccgcgagc cgaagatgga gagcgacaag ccgaagaaca tccagccaag
    14521 ttgagtgatg gtcgaccagg ccagcaaccg cttcatatcc tgctgtggca aatacatcag
    14581 aaagccataa agaatggtga ccagtgccat gaccatgcca acgccgccaa tcacatgcgg
    14641 gatattgccg ccgtcgataa ttgcgcgggc aaaaatgtaa acgcccactt tcaccatcga
    14701 tgcggcgtgg agataggcgc tgatcggtgt tggcgcttcc attgcatccg gtagccacgc
    14761 ttgcatcggt agctgggccg atttccccca cgcggcaaac aggatcccgc cataaaccag
    14821 ataacgtgcg tcgccgtgta actcgctcat tgcgctaagc gcaaacgttc cggtctgcaa
    14881 aaacagcgtg gcggcggcaa gatacaaccc caacgaaccg atatgggtga taagcaacgc
    14941 ttttagtgct gaacgctgcg ctttatcgct ctggtaataa ctgatcaacg cccaggagca
    15001 gccgccctaa tttcaaaaaa caacaactga ccgagcagcg tcgaggagag taccagtccc
    15061 gccatcgcgc cgataaacac cagcaaaaat gcgtaataac gattcgtgcc gttatgcggg
    15121 tgttcgcgat ttttatccgt cagataaccc gtggagtaga tcgtgaccag caaaccgaga
    15181 aacaccacca caaacagaat cagcgtactc acgcgatcaa tgaccaggcc aaacaatgcc
    15241 acatccccca cgttgaccaa cgtcaacgtg acggcaactt tatcggcctg ataaaacgcg
    15301 gagatcagcg acaacatgca cagcgtggtc agtgcggcga acaaaacccc cattcggcgg
    15361 cccgacgttg tggcgaaaac gaaacgacca gtgcgccgat aaaagacagc attaacgtcg
    15421 tcagagcaag attttccata ttccgtcagt gctccttaca gaccggttaa ccagaagacc
    15481 catgcaagcg cagcaaggct gaagccaagc caggtcacat ggtgaatgag taaaaaacgc
    15541 ccgcgtgcca gcgtgttttc tgcgattgag gccagtacaa aaatcagcaa aaccttgagc
    15601 agcgtaacga caagtgaagt cagcaggcag gcgagagaaa gttcttgcgc gcgcccaaag
    15661 ggcagaaaca gggccacaaa cagtgctgcc atcacgacct gtttcagccc cagcccccat
    15721 ttcgctagcg ccagcccggc accggaatat tcggtcagcg ggccttcctg taattcctgt
    15781 tctgcttcag caacatcaaa gggaattttt cccatctcaa tgaagcaggc aaaaccacag
    15841 gccagtaacg ccagtacggt ggttagcggc gagttccagc ccgttgccag cgtattgctg
    15901 atcatctcga tatgcgtgga acctgctatc agcgccaata ccagcagtga gagaatgagc
    15961 atgggttcga ccagaatgcc gagcgtcaac tcgcgactgg caccgactcc cgcaaacggg
    16021 cttccggtat ccagcccgga aagagcaaag aaaaaacgaa acagggcaag aagatagata
    16081 agggtgatca gatcgccgcc gcccgcaaaa ggggaaacgg taataaaaag tggtagggcc
    16141 atcgccagca ccagcatgct gctgattaat acccacggca tcaggcggaa catcagacct
    16201 gaagatatag gcgcaacttc ctggcgttta aacagtttgt ggatatcgcg ataatcctgc
    16261 cagatccccg gtccgcggcg ggagtgcatt cgcgcgcgta tctgccggga aatacccgta
    16321 aaaagtgggg ttagcatcag cagaatcacg gcctgtgcta acgcaaaaat gaccagatat
    16381 ccgtcgcaaa gagtttgtct catggtgatt tccttagacg gcaatagcga ttagcagcac
    16441 aaccagtgcg gcaaccacat acaggcaata gagacgaaag tcgccgcttt gcagatgctg
    16501 tatttctttg gccagccgtt gggtggcgct cacgatgggg cggatcaccc gctcatccca
    16561 gaagggttct gtgctctgag ccctggcggt gacgtgcgca agacctttat ttagcctcag
    16621 cgtagggtcg agctgttgtc gtagacgaaa tagcgcagaa aagaccacac gcagcggctg
    16681 catcacgcca ttgcctgacg gggccatcgc attttcccag ccgtagccgc atgcccaggc
    16741 atctcctgtg cgacgaaacg ctgcgcggcg cgaacgacac cacagccagt aaaggccagg
    16801 cagcaaaggc atcgccagta gtaacagcaa cagtaatgag ggggtgaccc gtgtatgaaa
    16861 cgtgccgggt acaagtgcta ttccgctggc gacagtgacg ggaggggtat tggtaaacgc
    16921 ataggcaata tgcattatct tcggtgccag ccaactggca ctaagcgcaa ttaatacgca
    16981 gagtgccgcg agcagtagca tggcgacgat catcgtattt ggcacttcct gtgcctcttc
    17041 agcgtgtgta ctgcgcggcg caccacagaa agtaataccg tacattttga cgaagcacat
    17101 tactgccagc ccaccagtga ctgccagcat taccatagca ataggacccg caagttgtag
    17161 cgctacggct tccacacgac ttagtgagaa cagcgactgc caggtgtacc attcgctgat
    17221 aaaaccattc agcggaggaa tggctgatat cgcgaggcaa ccaatcaggc atgctgcggc
    17281 tgtccacggc atccgtttcg ccagtgcccc cattttttcc atgtcgtggg tatgcaaacg
    17341 cgaaataatc gcacccgcgc cgagaaatag cagccctttg aacagcgcat ggttgagcag
    17401 atgaaacagt gcgccgagca gtccaaccac ggtgagcagc gggtcgtgca gtgacagacc
    17461 gtccatcgcc acaccgaccg ccagcaaaat aatgccgacg ttttcgacgg tactccaggc
    17521 cagcagccgt ttgatatcct gttccgccag tgcatacagc acgcccagga gcgcggagat
    17581 tgcgccgacc gccatcacca gagtgcccca ccacagaggc aaacccgttt gcgccagcag
    17641 atccatcgct actttcagga tgccgaaaat accgattttg accattacgc cagacatcaa
    17701 cgccgaagcg tgcgatggtg cggcagggtg agcgcgcggc aaccagctgt gcaacggcat
    17761 catcccggct ttcgcgccaa aaccgaaaaa ggccagcaga aacaccgccg acgccagccc
    17821 cggagaaagt gaaagcgtgc gaaactggca aaatcgaggc tgccgctttc gcgccccatc
    17881 agcaagaagg cgatcataat cagcaccgat ccggcgtggg cgataaaaaa gtagagcatg
    17941 ccagcgttga tcgacgtttt atcctgcctg gcaatgacca gaaaccagga agacagcgac
    18001 atcatttcaa acagcacgat gaaccaaaaa gcgttgtcca tcaccagcag ggcaaccatc
    18061 gatgcgatga aaagattcat aaagaagccc atcgccgccg cgcctttgcc ctcgtattcg
    18121 cgcatataag tcaatgaata gagcgaacaa accaccacca gcaaggagat aaccagtacc
    18181 ataaatgccg agaggctatc cattcgcagg gttaaatcgg caaagtcaaa aggggtggca
    18241 aaatgggcga ctaaagttac accagcatgt aattgcgtaa ttccgctgat gatgccaatc
    18301 acgccgccca ccagcgatgt gatgccggaa agcttaatag ccagtctgtc cagaccgagt
    18361 aaaaacagcg aagccagact ggcaaacaga tagaggatca gcgaccaggt taataattgc
    18421 aaggcatcca ttagtgttgc tcctgagtga gggaaagggg atcctccccg ccattggcaa
    18481 accagcttgc ggcaaggcgc tgtttttctt tcatctgacg ttgcaggcta tcgtcggtga
    18541 tcagccgtaa cgcctgattc gggcaagcgc gaacgcaggc cggaccttct ggcaagaaat
    18601 cacacaggtc gcatttcacc gcgacggtct ggacacccgg ttcccggcgc agcaacgcat
    18661 gttggggcag cacgttttct tcgccgtctt tgagtgagcc ttcagcctga aaaacatatt
    18721 gcgcatgggc attcaccgga cggcttcctg aagcgctgat tgcgccaaat gggcagacca
    18781 cggcgcaaag cttgcagcca atacagaggc tttcgttgag ttggatcgca tcatccctct
    18841 gggagatggc attaaccggg cagacctgca ggcaaggggc ttcctcacag tgatgacaca
    18901 cgacaggggc agtgagtgtt gacgtcttcg ccagggccag gcgcgggtgt tgctgtaaac
    18961 cttgcgtttt atgcacgtcc gaacaggcag cgagacaggt attacatcct gtacaccaca
    19021 gtggttcggc caccacaaag cggttcatgt cggctccttg aaaatgatat gaaacattgc
    19081 atttcatatt gcataaagga tgccattctg attaactcaa tatattcaat aagttatgta
    19141 ttgactggat tctgaacggt acaaaaatga tggtcattaa cgttcagcaa aaaatggatt
    19201 cgacacccgt gtcgcagggg aaagcgagaa tgtgatcttg atcacgagaa gtgaggtatg
    19261 ctggccgcaa gcgcagccag cacggaatgg agcaaagtaa tcaggcgtgt tctttcagcc
    19321 agttcagcac aacgtcgtgg tgattgctgg ttttgaaatc gtcaaagaca tgttcgattt
    19381 tgccatcagc gtcaatcagg aagctgatgc gatgaatgcc atcgtaggtt ttgcccatga
    19441 aggacttttc accccagacg ccgaattgtt cgcacacctg gtggtcctca tcagacagga
    19501 gcgtaaagtt aagcagctct ttttccgcaa aacgggagag tttttcgggt ttatcggtgc
    19561 tgatacccag cacatcaacg cccgcttttt tcaactcatc catgttatcg cgtaagccgc
    19621 aggcctgtac ggtacagccg ggggtcatgg ctttcgggta gaaataaacc agaacacgct
    19681 gtccctggaa gtcggtcaaa ttaacttgtt ctccgtcttg atccggcaag ctaaatttcg
    19741 gtgcgatatc accggctttc agtggattca ttacttaact ccatcctgtt catcatgttg
    19801 ggaataattg acgacgttaa tactgccttg tgcattgagt tctgtacata gggctttgaa
    19861 cgcttgctca atatttgctg cgtccgcaga tgcggggctg tgggcggtta tctgaatatg
    19921 caactgcgca gcccgttcat tttcagcagg ttgcgtgcgc gacaccagct ccgcaatgtt
    19981 catatgatgc gcgtcgaaaa gtgctgtgaa gcgttcaatt aaatgcgggg agtctgccac
    20041 atcgacctga acccagacag atgctggcat tggcggacgc ggacgcgccg tcgtgcgctt
    20101 catcacgatt aaaagatcca gttcggcacc tttcaacggt aacgttgatt caatcagagt
    20161 aatggcattc catgaaccgg aaagcagcat aataaacgtg aactcttctc ccagcatcgc
    20221 caggcgactg tcttcaatat tacagccgca actactgaca tgacgggtga tggtgttcac
    20281 aattccaggg cgatcggcac ccaacgcagt gatcaccaga taatgttgcg atgacagtgt
    20341 caaactggtt attcctttaa ggggtgagtt gttcttaagg aaagcataaa aaaaacatgc
    20401 atacaacaat cagaacggtt ctgtctgctt gcttttaatg ccataccaaa cgtaccattg
    20461 agacacttgt ttgcacagag gatggcccat gttcacggga agtattgtcg cgattgttac
    20521 tccgatggat gaaaaaggta atgtctgtcg ggctagcttg aaaaaactga ttgattatca
    20581 tgtcgccagc ggtacttcgg cgatcgtttc tgttggcacc actggcgagt ccgctacctt
    20641 aaatcatgac gaacatgctg atgtggtgat gatgacgctg gagctggctg atgggcgcat
    20701 tccggtaatt gccgggaccg gcgctaacgc tactgcggaa gccattagcc tgacgcagcg
    20761 cttcaatgac agtggtatcg tcggctgcct gacggtaacc ccttactaca atcgtccgtc
    20821 gcaagaaggt ttgtatcagc atttcaaagc catcgctgag catactgacc tgccgcaaat
    20881 tctgtataat gtgccgtccc gtactggctg cgatctgctc ccggaaacgg tgggccgtct
    20941 ggcgaaagta aaaaatatta tcggaatcaa agaggcaaca gggaacttaa cgcgtgtaaa
    21001 ccagatcaaa gagctggttt cagatgattt tgttctgctg agcggcgatg atgcgagcgc
    21061 gctggacttc atgcaattgg gcggtcatgg ggttatttcc gttacggcta acgtcgcagc
    21121 gcgtgatatg gcccagatgt gcaaactggc agcagaaggg cattttgccg aggcacgcgt
    21181 tattaatcag cgtctgatgc cattacacaa caaactattt gtcgaaccca atccaatccc
    21241 ggtgaaatgg gcatgtaagg aactgggtct tgtggctacc gatacgctgc gcctgccaat
    21301 gacaccaatc accgacagtg gtcgtgagac ggtcagagcg gcgcttaagc atgccggttt
    21361 gctgtaaagt ttagggagat ttgatggctt actctgttca aaagtcgcgc ctggcaaagg
    21421 ttgcgggtgt ttcgcttgtt ttattactcg ctgcctgtag ttctgactca cgctataagc
    21481 gtcaggtcag tggtgatgaa gcctacctgg aagcggcacc gcttgcggag cttcatgccc
    21541 cggctggaat gattttgccg gtgacctccg gtgattatgc aatcccggtg accaacggta
    21601 gtggtgctgt cggtaaggcg ctggacattc gtccaccagc ccagccgctg gcactggttt
    21661 ctggcgcgcg tacccagttc acgggcgata ccgcttcatt gctggtggaa aatggtcgtg
    21721 gcaatactct gtggccgcag gtggttagcg tgctgcaggc gaaaaactac accatcaccc
    21781 aacgtgatga tgctggtcag acactgacca ccgattgggt acaatggaac cgtctggacg
    21841 aagacgagca gtatcgtggt cgttatcaaa tctctgttaa gccgcagggt tatcagcagg
    21901 cggttacggt taaactgctg aacctggaac aggcgggcaa accggttgca gacgcggctt
    21961 ccatgcagcg ttacagcacg gagatgatga acgttatttc cgccggtctg gataaatctg
    22021 ccactgacgc cgcgaacgct gcgcaaaatc gtgcctccac cactatggac gtacaaagtg
    22081 cagctgatga caccggttta ccgatgctgg tcgtacgcgg gccgttcaat gtggtttggc
    22141 aacgtctgcc agcggcgctg gaaaaagtgg gcatgaaagt gaccgacagc acccgttcgc
    22201 agggtaacat ggccgtaact tataagccgc tgtctgacag cgactggcag gaactgggcg
    22261 cgagcgatcc aggcctggca tccggtgact ataaactgca ggttggcgat ttagataacc
    22321 gcagcagcct acagttcatc gatccgaaag gtcatactct gactcagagt cagaacgacg
    22381 cgctggtatc tgtcttccag gctgcgttta gcaagtaaaa atacagggct ggaatcatcc
    22441 ggcccttttt tctgatatga tacgcaaacg tgtgcgtagg caggaaaacg cgattttagc
    22501 ggtaattcgc acgaaatttg tttgtcggac gtagttcgaa taaggcgttc acgccgcatc
    22561 cgacaaaaca tccggcacac cagacagcaa aagattttaa aacgttaatt cacacccagg
    22621 agtgataaag atgcaaaagc aagctgagtt gtatcgtggt aaagcgaaaa ccgtatacag
    22681 cacggaaaac ccggacctgt tggtgctcga attccgcaat gatacgtcag caggggatgg
    22741 cgcgcgcatt gagcagtttg atcgcaaagg tatggtgaac aacaagttca actacttcat
    22801 tatgagcaaa ctggctgaag cgggtatccc gactcaaatg gagcgtctgc tctccgatac
    22861 cgaatgtctg gtgaaaaagc tggatatggt gccggttgag tgtgtcgtgc gtaaccgtgc
    22921 tgctggctct ctggtgaaac gtcttggaat cgaagaaggt attgagctga acccgccgct
    22981 gttcgatctg ttcctgaaaa acgacgccat gcacgatccg atggtcaacg aatcttactg
    23041 cgaaaccttt ggctgggtga gcaaagagaa cctggcgcgt atgaaagagc tgacctacaa
    23101 agcgaacgac gtgctgaaaa aactgttcga tgatgctggt ctgattctgg tcgacttcaa
    23161 gctggagttt ggcctgtaca aaggtgaagt ggtactgggc gatgagttct ccccggacgg
    23221 cagccgcctg tgggacaaag aaacactgga gaaaatggac aaagaccgtt tccgccagag
    23281 cctcggtggc ctgatcgaag cctatgaagc cgtcgcccgc cgcctgggtg tacagctgga
    23341 ctgatttttc tggttgcttg tttctttagc cggagggtac tccggctaaa tgtctttgat
    23401 acccccgcta caatacttct cattatctcc ctttgacagg aaagtcatac caggccaata
    23461 tgtatataca taacgtatat cccaaaaagg gggggctatg gaaactactg tatttttaag
    23521 taatcgcagt caggccgtca gattaccgaa agcggtcgct ctgccagaag acgttaagaa
    23581 agtagaaatt attgcgattg gtcgtacaag aatcatcact cccgccggtg agtcctggga
    23641 cagttggttt gatggcgaaa atgtgagtgc ggattttatg gatatccgtg atcaacccgc
    23701 aatgcaggaa agggaatcat tttgatgctg aaattcatgc tggataccaa tatctgtatt
    23761 ttcactatta agaataaacc agtacatgtc agagagcggt ttaacctgaa ttctggccgg
    23821 atgtgtataa gttctgtaac attgatggag cttatctatg gggcagaaaa aagtcagatg
    23881 ccagaacgca atttggcggt tattgaaggt ttcgtatctc gtttggtagt gcttgattat
    23941 gatagtgctg ctgcgacgca taccgggcaa atcagggccg agcttgcccg gcagggaagg
    24001 cctgttggac cttttgacca gatgatagcg ggacatgctc gcagtcgcgg tttgattgtg
    24061 gttaccaaca atacccgaga gtttgagcga gtggcgggaa tcagactaga agactggagc
    24121 taacattctc ttttacttct aacattcgta aattatataa cgtggcatga agccttaatt
    24181 cctccgccat tttatcccgc tttcctgtgg taatcttcct ctgaaccacc tacgatcatt
    24241 tctataatga atatattgtt gaggtatcta tgcgttggca agggcgacgt gaaagtgaca
    24301 atgttgaaga caggcgcaac agctctggcg gtccgtcaat gggcggtccc ggtttccgtc
    24361 tgccgagcgg taaaggcggg ctgattttac tgatagtcgt gctggttgca ggctactatg
    24421 gcgttgattt aaccgggttg atgaccgggc agccggtttc tcaacaacaa tcaacgcggt
    24481 caattagccc taatgaagac gaagccgcaa aattcacctc ggtgattctg gcaaccacgg
    24541 aagacacctg gggacaacag ttcgagaaga tgggtaagac ctatcagcaa ccgaagctgg
    24601 tcatgtaccg tggaatgacg cgcaccggct gcggggctgg tcagtccatt atggggccgt
    24661 tctattgccc ggcggatggc acagtttata tcgatctctc cttctatgat gacatgaaag
    24721 acaaacttgg cgcggatggc gattttgccc aggggtacgt tatcgcccat gaagtcggtc
    24781 atcatgtgca gaaactgtta ggcatcgagc cgaaagttcg tcaactgcaa caaaacgcga
    24841 cgcaggcgga agtaaaccgc ttatctgtgc gtatggaact ccaggccgac tgttttgccg
    24901 gtgtctgggg gcatagtatg cagcagcaag gcgttctgga aactggcgat ctggaagagg
    24961 cgctgaatgc ggcgcaggcc atcggcgatg accgtttaca acagcaaagt caggggcgag
    25021 tagtaccaga cagtttcaca catggcactt ctcagcaacg ctacagctgg tttaaacgtg
    25081 gtttcgacag cggcgatccg gcacaatgca atacttttgg taaaagcatt taatttccgg
    25141 ggcagggatg gctgaactga ctgcgcttta cacattaaca gcgcaaatga aacgtgaagg
    25201 gatccgccgc ttgctggtgt tgagcgggga agagggttgg tgttttgatc atgcgcttaa
    25261 gttgcgtgat gccttacctg gcgactggct gtggatttcg ccgcagccag atgctgaaaa
    25321 ccactgttca ccctcggcgc tacaaacttt acttgggcgc gagttccggc atgcggtatt
    25381 cgacgcccgc cacggctttg atgccgctgc ctttgcagca cttagcggaa cgttgaaagc
    25441 gggaagctgg ctggttttgt tactccctgt atgggaagag tgggaaaacc aacctgatgc
    25501 cgactcgttg cgctggagtg attgccctga ccctattgcg acgccgcatt ttgtccagca
    25561 tctcaaacgc gtacttacgg cggataacga cgctatcctc tggcggcaaa accagccgtt
    25621 ctcgttggcg cattttactc cccgtactga ctggcacccc gcgaccggcg caccacaacc
    25681 agaacaacag caactcttac agcagctact gaccatgccg ccgggcgtgg cagcggtaac
    25741 ggctgcgcgt gggcgcggta agtcggcgct ggcagggcaa ctcatttctc gtattgcggg
    25801 cagtgcgatt gtcaccgcgc ccgcaaaagc ggcaacggat gtactggcac aatttgcggg
    25861 cgagaagttt cgctttattg cgccggatgc cttgttagcc agcgatgagc aagccgactg
    25921 gctggtggtc gacgaagccg cagccatacc tgcgccgttg ttgcatcaac tggtatcgcg
    25981 ttttcctcga acgttgttaa ccactacggt gcagggctac gaaggcaccg gacgtggttt
    26041 tttgctgaaa ttttgcgctc gctttccgca tttacaccgt tttgaactgc aacagccgat
    26101 ccgctgggca cagggatgcc cgctggaaaa aatggttagt gaggcactgg tttttgacga
    26161 tgaaaacttc acccatacac cacaaggcaa tatcgtcatt tccgcatttg aacaaacgtt
    26221 atggcgaagc gagccagaaa cgccgttaaa ggtttatcag ttattgtctg gtgcgcacta
    26281 ccggacttcg ccgctggatt tacgccggat gatggatgca ccagggcaat attttttaca
    26341 ggcggctggc gaaaacgaga ttgccggagc gctgtggctg gtggatgagg ggggattatc
    26401 tcaacaactc agtcaggcgg tatgggcagg ttttcgtcgc ccgcggggta atctggtggc
    26461 ccagtcgctg gcggcgcacg gtagcaatcc actggcagcg acattgcgtg gacggcgggt
    26521 cagccggata gcagttcatc cggcgcgtca gcgggaaggc acagggcagc aacttattgc
    26581 tggtgctttg caatatacgc gtgacctcga ctatctttcg gtgagttttg gttacaccgg
    26641 ggagttatgg cgtttctggc accgctgcgg ttttgtgctg gtgcggatgg gtaatcatcg
    26701 tgaagccagc agcggttgct atacggcgat ggcgctgtta ccgatgagtg atgcgggtaa
    26761 acagctggct gaacgtgagc attaccgttt acgtcgcgat gcgcaagctc tcgcgcagtg
    26821 gaatggcgaa acacttcctg ttgatccact aaacgatatc gtgctttctg acgacgactg
    26881 gcttgaactg gccggttttg cttttgctca tcgtccgcta ttaacgtcgt taggttgctt
    26941 attgcgtctg ctacaaacca gtgaactggc attaccggcg ctgcgtgggc gtttacagaa
    27001 aaatgccagt gatgcgcagt tatgtaccac acttaaactt gcaggccgca agatgttact
    27061 ggtccgtcag cgggaagagg tcgcgcatgc gctgttcgca cttaatgatg ttcgcactga
    27121 gcgtctgcgc gatcgcataa cgcaatggca attttttcac tgactccttc aattttctcc
    27181 catggtcatt gtgagccagc ggcgtaaaat taatgacatc agttaaggag ttcgccatga
    27241 aacatgacca ttttgttgtt caaagcccgg ataaacctgc gcaacagttg ttgctgcttt
    27301 tccacggtgt cggggataac ccggtggcaa tgggggaaat aggtagctgg tttgcaccgc
    27361 tgtttcctga tgcgctggtg gtgagtgtcg gtggcgcgga gccgagcggt aatccggcgg
    27421 ggcgtcagtg gttttcggtg cagggtatta cggaagataa tcgccaggcg cgtgtggatg
    27481 cgatcatgcc gacgtttatt gaaacggtgc gctactggca gaaacagagc ggagtagggg
    27541 ctaacgccac ggcgctcatc ggtttctctc aaggcgcgat tatggcgctg gagagcatta
    27601 aagccgaacc gggccttgct tcacgcgtca ttgcttttaa cggacgctat gcgagcctgc
    27661 cggaaactgc gtctacagcc acgacaattc acctgattca tggcggtgaa gatccggtta
    27721 tcgatctggc gcatgcggtt gcggcgcagg aagcgttaat cagtgccggt ggtgacgtga
    27781 cgctggatat cgtggaggat ttaggccatg cgatcgacaa tcgcagtatg caatttgccc
    27841 tggatcactt gcgttatacc attccgaaac attatttcga cgaagcgcta agtggcggta
    27901 agcctggcga tgatgatgtg attgaaatga tgtaaaagac agattacctg gcctgataag
    27961 acgcgtgagc gtcgcatcag gcatcgttca ccattgccgg atgcggcgtt aacgccttat
    28021 ccggcctacg gttgagcact cagcttactt cttcggttga tcctttttcg gccagtcgtc
    28081 gtcatcgtcc cacttatcgt tgaaatcacg atgcggggga agctccggct tgttggcgag
    28141 aaatttctta tggtcgacgc gcttgagatc tttaatcacg ttcagcagga cgcctaccaa
    28201 aaagacaatc accagaatcc accaatattt agccagccag tccatgctta tttcctcttg
    28261 cagaaccact catcaggcga cgagctgttc catgatacgt tgatacatac gggcaagtag
    28321 ctgcaggtcg gcagcgttca cacattcatt aattttatga atagtggcat tgaccggccc
    28381 gagttccacc acctgcgccc ccatgcgagc aataaagcgc ccgtcggacg ttccgcctgt
    28441 ggtcagtagc tgcggtttaa tttcattata gtgctcaacc gcgttaacga ccgcatccac
    28501 cagtttaccg cgcgcggtca aaaatggctg cccggaaagc caccaatcca ccgtatagcg
    28561 cagttgatgt ttttcaagca gggcaagcac ctgcgctttg atcatctcat cagtcagttc
    28621 ggtgctgaag cggaagttaa actgcacaaa cagttcaccc ggaataacat tgttactgcc
    28681 cgttcccgcc tgaatattag caatctgcat actggtcgcc gggaaaaatt cattgccctg
    28741 atcccactca atagccacta attcattaag gaaaggtgct gcgcgatgta ctggattgtc
    28801 agccagatgc gggtaggcca catgcccctg aacgccatga atggtaaggt tgcaggttaa
    28861 cgatccgcga cgaccatttt tcaccacatc accaaccact tcgatactcg acggttcgcc
    28921 gaccaggcag taatcgagac gctcattacg tgccatcaac gcttcgacga cttttaccgt
    28981 accgttgtgg gcactggctt cttcatcaga ggtgatcaga aatgccagtc gccccgtatg
    29041 gttgggatgt tgtgcgacaa agcgttctgc cgctaccacc atcgccgcca gcgagccttt
    29101 catatctgcc gcaccgcgcc cgaataacat gccatcacga atggtgggtt caaacggcgg
    29161 attgatccaa cgatcggcgt cgccaggcgg caccacgtcg gtatgcccgg caaaggctaa
    29221 cgtttcacct tgcccacgcc atgcccaaaa attctgcgta tcggcaaagt ccatgcgttc
    29281 aacggtaaaa ccgatcgcct gcaaacgttc aatcaacaaa gcctggcatc ctgcgtcatc
    29341 aggactcagg gaagggcggc gaataagctg ttgtgtcagc tcaataaccg ggcacgacat
    29401 agactacacc tcatggaaaa attgctgata actggaatca ctgaaaccca gcagcatagg
    29461 cttaccaggc gcgcagagca atggacgttt gataattgca ggcatttcag tcattaatgc
    29521 cgccgcagag gccgcatcgg tgattttatt gcgggtggtt tcgtccagtt tacgccaggt
    29581 tgtaccacgg gtgttcagta acgcttccca gcctaattcg ttgataaaac cgttcaataa
    29641 ttcgctgtcc agcccatcga cgcggtaatc atgaaaacga tagtcgatgt tattggcttc
    29701 cagccaacgg cgagcctttt taatggtgtc acaatttttg atgccgtaaa gtgtaaccat
    29761 aatgaatcct ttttcgatct atgagaataa atactcattt aagggtgaaa acattttgcc
    29821 tgcaa
//
LOCUS       DAZGVQ010000051        29543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000051 DAZGVQ010000000
VERSION     DAZGVQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.051"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            86..628
                     /gene="zapC"
                     /locus_tag="ACHLAG_002143"
     CDS             86..628
                     /gene="zapC"
                     /locus_tag="ACHLAG_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFP0655376.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(625..1734)
                     /gene="ycbX"
                     /locus_tag="ACHLAG_002144"
     CDS             complement(625..1734)
                     /gene="ycbX"
                     /locus_tag="ACHLAG_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFP0655377.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRTGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            1978..4086
                     /gene="rlmKL"
                     /locus_tag="ACHLAG_002145"
     CDS             1978..4086
                     /gene="rlmKL"
                     /locus_tag="ACHLAG_002145"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFP0655378.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGIPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEDIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4098..6005
                     /gene="uup"
                     /locus_tag="ACHLAG_002146"
     CDS             4098..6005
                     /gene="uup"
                     /locus_tag="ACHLAG_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0655379.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6135..7388
                     /gene="pqiA"
                     /locus_tag="ACHLAG_002147"
     CDS             6135..7388
                     /gene="pqiA"
                     /locus_tag="ACHLAG_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFP0655380.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7393..9033
                     /gene="pqiB"
                     /locus_tag="ACHLAG_002148"
     CDS             7393..9033
                     /gene="pqiB"
                     /locus_tag="ACHLAG_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFP0655381.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            9030..9593
                     /gene="pqiC"
                     /locus_tag="ACHLAG_002149"
     CDS             9030..9593
                     /gene="pqiC"
                     /locus_tag="ACHLAG_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFP0655382.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            9849..10016
                     /gene="rmf"
                     /locus_tag="ACHLAG_002150"
     CDS             9849..10016
                     /gene="rmf"
                     /locus_tag="ACHLAG_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFP0655383.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(10086..10604)
                     /gene="fabA"
                     /locus_tag="ACHLAG_002151"
     CDS             complement(10086..10604)
                     /gene="fabA"
                     /locus_tag="ACHLAG_002151"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFP0655384.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10673..12433)
                     /gene="ycbZ"
                     /locus_tag="ACHLAG_002152"
     CDS             complement(10673..12433)
                     /gene="ycbZ"
                     /locus_tag="ACHLAG_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0655385.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12619..13071
                     /gene="matP"
                     /locus_tag="ACHLAG_002153"
     CDS             12619..13071
                     /gene="matP"
                     /locus_tag="ACHLAG_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFP0655386.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13147..14187)
                     /gene="ompA"
                     /locus_tag="ACHLAG_002154"
     CDS             complement(13147..14187)
                     /gene="ompA"
                     /locus_tag="ACHLAG_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFP0655387.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(14544..15053)
                     /gene="sulA"
                     /locus_tag="ACHLAG_002155"
     CDS             complement(14544..15053)
                     /gene="sulA"
                     /locus_tag="ACHLAG_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFP0655388.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15272..15901
                     /gene="sxy"
                     /locus_tag="ACHLAG_002156"
     CDS             15272..15901
                     /gene="sxy"
                     /locus_tag="ACHLAG_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFP0655389.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15864..18026)
                     /gene="yccS"
                     /locus_tag="ACHLAG_002157"
     CDS             complement(15864..18026)
                     /gene="yccS"
                     /locus_tag="ACHLAG_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFP0655390.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAIRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDEAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(18036..18482)
                     /gene="yccF"
                     /locus_tag="ACHLAG_002158"
     CDS             complement(18036..18482)
                     /gene="yccF"
                     /locus_tag="ACHLAG_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFP0655391.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            18605..20659
                     /gene="helD"
                     /locus_tag="ACHLAG_002159"
     CDS             18605..20659
                     /gene="helD"
                     /locus_tag="ACHLAG_002159"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFP0655392.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(20691..21149)
                     /gene="mgsA"
                     /locus_tag="ACHLAG_002160"
     CDS             complement(20691..21149)
                     /gene="mgsA"
                     /locus_tag="ACHLAG_002160"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFP0655393.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(21245..21907)
                     /gene="yccT"
                     /locus_tag="ACHLAG_002161"
     CDS             complement(21245..21907)
                     /gene="yccT"
                     /locus_tag="ACHLAG_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFP0655394.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            22080..22493
                     /gene="yccU"
                     /locus_tag="ACHLAG_002162"
     CDS             22080..22493
                     /gene="yccU"
                     /locus_tag="ACHLAG_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFP0655395.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(22538..22855)
                     /gene="hspQ"
                     /locus_tag="ACHLAG_002163"
     CDS             complement(22538..22855)
                     /gene="hspQ"
                     /locus_tag="ACHLAG_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFP0655396.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(22913..24103)
                     /gene="rlmI"
                     /locus_tag="ACHLAG_002164"
     CDS             complement(22913..24103)
                     /gene="rlmI"
                     /locus_tag="ACHLAG_002164"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFP0655397.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECSIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            24198..24476
                     /gene="yccX"
                     /locus_tag="ACHLAG_002165"
     CDS             24198..24476
                     /gene="yccX"
                     /locus_tag="ACHLAG_002165"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFP0655398.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(24473..24802)
                     /gene="tusE"
                     /locus_tag="ACHLAG_002166"
     CDS             complement(24473..24802)
                     /gene="tusE"
                     /locus_tag="ACHLAG_002166"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFP0655399.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(24893..25552)
                     /gene="yccA"
                     /locus_tag="ACHLAG_002167"
     CDS             complement(24893..25552)
                     /gene="yccA"
                     /locus_tag="ACHLAG_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFP0655400.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(25960..26979)
                     /locus_tag="ACHLAG_002168"
     CDS             complement(25960..26979)
                     /locus_tag="ACHLAG_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0655401.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRTIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRSA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(26957..27199)
                     /locus_tag="ACHLAG_002169"
     CDS             complement(26957..27199)
                     /locus_tag="ACHLAG_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFP0655402.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            complement(27267..>29543)
                     /locus_tag="ACHLAG_002170"
     CDS             complement(27267..>29543)
                     /locus_tag="ACHLAG_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP0655403.1"
                     /translation="GIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPMNVNFDKL
                     SPEVQNAVLVKFDTCENITVDMVISAQELLQEDMATFGGHIVEAFMKMPEVNSMYPEL
                     KLHAIGWVKHKCEPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARSRVNQQNT
                     ENAAEKNGAVTVAVRREYKQTWKTLDNELACALWPGDVDAGNIDGSIHRWAKNEVIDN
                     DREDWKRISASMRKQPEALGYDRQTIFGLVRERPIDIHKDPVALNKYITEYLTTKGVF
                     EDEGTNQSTTNTLPSPVPEADAVETAMPDNEKTECKVEVEPSVEREGPFYFLFTDKDG
                     EKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGSQQNTDASDTTAQPDLVKVT
                     ADEVNKIMQAASISQPDADKLLAASRGEFVAGISDPNDPKWVKGIETRDSVDQNQQES
                     EQNDQKAEQNSPNALQNEPETKQPEPVVQQEPEKICTACGQTGGGNCPDCGAVMGDAT
                     YQETFDEEYQVEVQEDDPEEMEGAEHPHKENAGSAQDHASDSETGETADPLITVNGHH
                     VITSTSRLWHHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGV
                     IDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQVWGNGANFDN
                     TILRRSYERQGIPCPWRYYNDRDVRTIVELGKAIDFDARTVTPFEGERHNALDDARYQ
                     AKYVSAIWQKLIPSQADF"
BASE COUNT         7377 a         7188 c         7671 g         7307 t
ORIGIN      
        1 cattttgtac ttttattgtt gaggttatta agcgaagcga caatggattg tgttgctgcg
       61 gttttatagg gtaggggaga ggcagatgcg aattaaacca gacgataact ggcgttggta
      121 ttacgatgaa gagcacgatc gaatgatgct cgatttagcc aatggtatgc tatttcgctc
      181 acgttttgcg cgcaagatgt tgactccaga tgccttttct cccgcaggct tttgcgttga
      241 cgacgccgcg ctctatttct cttttgaaga aaagtgccgc gattttaatt tatctaaaga
      301 acaaaaagcc gaactggtgc tgaatgcact ggtagcaatt cgctatctca aaccgcaaat
      361 gccgaaaagc tggcattttg tttcccatgg tgaaatgtgg gtgcccatgc cgggcgatgc
      421 ggcctgcgtt tggttgagcg atactcacga gcaggtcaat ttgctggtcg ttgaatctgg
      481 cgaaaatgcc gcactatgcc tgctggcaca accctgcgtt gttattgcgg gtcgcgcgat
      541 gcagttgggc gacgccatta aaatcatgaa cgacaggctg aaaccgcagg ttaatgttga
      601 cagcttcagc ctcgaacagg cagtctaacg cgccaactta agtgcagtct tcggtacaca
      661 gctacagcaa agaatggtgc catcatcgcc cattgctgat tttttcagcg gcgtgacttc
      721 gccttctaaa agctgaacac ggcaacttcc acaaatgccc gtgcggcaag aataagggat
      781 acgaattccc tgattttcta attgctccag caacacctgt tggttatttc cacgaaatgc
      841 ctgtccctgc cagtcaatat ctacatttgc gtccggttgt tgcgtgatgt tggcggtatc
      901 atcagcggca gctgcgccgt aaatttttgc cggagccgtt gccagaattt ccacctcatc
      961 gccaacgcga atcacgccgc tattacgggc aattaaattc tgaccaaaat cgacatcgcc
     1021 gttatcctgg gcagtgcgga aagattgcaa tgtttttaat ggttcgcctg ccggatgttt
     1081 ttgccctttt tctgggctga cggtggtgaa aatacagcgg ctacaaggtt taaccacatc
     1141 aaacaccaca tcaccaatgc gaatcacttt ccagcgatct tcttcccagg ctgacgcgcc
     1201 ggaaaccacc agattggggc ggaattgctc catttttaca ctggccggac aacgttgttg
     1261 gagatcacgt aacgaggctt cgttagcaag aaggtaagga tagccatcag caaatgacag
     1321 aggtacagtg ttgtggcgtt tcacgcgccg ggtcatttgt ggccccaccc agcgtaattg
     1381 cacttcgcgg gagaaaaatc cacttagcca tttgttgatc gcgtctggcg caattcgcgc
     1441 ggtaaaatgt gtgccccaaa cttcggttgg tgcgtcttgt gtggcgaaat cagcaaaacg
     1501 aacatatgcg ctactgccat ctggtgcggt gagatgcaag ccatcatgca caggtgaagg
     1561 agtaaaccgt accatctggg gaaactggcg agcggtaata aacgtaccgt caggttccgt
     1621 gatcataaag atgcgatcga aggccagacc actgacatca gccagagcat gtgtaagacc
     1681 aatgccgcgc atcgatttaa caggatgaat aaaaagccgg gttaatgtcg ccacggtgcg
     1741 gtcctcaaat gaaaataagc cctcaacttt atgacatgac gcgcttatta gctataatgc
     1801 gcaacaattt tcttagcgcc tgtcccatta ggctatttta tttgtcagtt tggccttggg
     1861 ttgtgctcga aatcctcacg tacttgtgta cgctccggtt tctccgcgct gtccatgtcc
     1921 agactgactg caacaattac gcctactgcg gtaggctctt agagtaaaag tgacgatatg
     1981 aattctctgt ttgccagtac ggcccgtggg ctggaagagc tgttaaaaac tgaactggaa
     2041 aacctggggg ccgttgaatg ccaggtggtt cagggtgggg tccatttcaa gggcgacaca
     2101 cggcttgttt accagagcct gatgtggagc cgcctggcct cgcgtattat gttgccgctg
     2161 ggcgagtgta aggtttacag cgatttagac ctctatctcg gtgttcaggc gatcaactgg
     2221 acagagatgt ttaatcctgg cgcgaccttc gctgtccact tcagtggttt gaatgacacc
     2281 atccgcaaca gtcagtacgg cgcgatgaaa gtgaaagacg cgatcgtcga tgctttcacg
     2341 cggaaaaatc tgccgcgtcc aaatgttgat cgcgatgcgc cggatatccg cgttaacgtc
     2401 tggctgcata aagaaaccgc cagtatcgct cttgatctga gtggtgatgg tttacatctg
     2461 cgtggctatc gcgatcgtgc tggtattgcg ccgatcaaag aaaccctggc agccgctatt
     2521 gtgatgcgat ccggctggca gccaggaata ccgctgctcg atccgatgtg tggttccggt
     2581 acgttgctga ttgaagcggc gatgctagcg accgatcgcg caccaggctt gcaccgtggg
     2641 cgttggggct ttagcggctg ggtgcagcat gatgaagata tctggcagga agtgaaagcg
     2701 gaagcgcaaa ctcgtgcccg taaaggcctg gctgagtata gctctcattt ttacggttcg
     2761 gacagcgacg cacgggtgat tcaacgtgcg cgcactaacg cccgtcttgc ggggattggt
     2821 gaactgatca cctttgaggt gaaagatgtc gcgcaactga ccaatccgct gccgaaaggg
     2881 ccgtacggta cagtgttgag caacccgcca tacggggaac gtctggacag cgaaccggcg
     2941 ctgattgcgc tgcatagcct gctgggtcgg atcatgaaaa accagtttgg tggctggaat
     3001 ctctctttgt ttagtgcctc gccggatctg ctaagctgcc tgcagctgcg tgcagacaaa
     3061 cagtacaagg cgaaaaacgg cccgctggac tgcgtacaga aaaattacca cgttgccgaa
     3121 agcactccag acagcaaacc ggcgatggta gcggaagact acgccaaccg cctgcgtaag
     3181 aacctcaaaa aattcgagaa gtgggctcgt caggaaggaa ttgaatgtta ccgcctgtat
     3241 gacgccgatc tgccggaata taacgttgcc gttgaccgtt atgccgactg ggtggtggtg
     3301 caggagtatg caccgccaaa aactattgat gctcacaaag cgcgtcagcg tctgttcgat
     3361 attatcgctg caaccatttc ggtactgggg attgcgccaa acaaactggt gctgaaaacc
     3421 cgtgaacgtc agaagggcaa aaatcagtac cagaaactgg gcgagaaggg cgagtttctg
     3481 gaagttaccg aatataacgc tcacttgtgg gtgaatctga cggattacct cgataccggt
     3541 ctgttcctcg atcaccgtat cgcccgtcgt atgctcggcc agatgagcaa aggcaaagat
     3601 ttcctcaacc tgttctctta taccggcagc gccaccgtgc atgcgggatt aggcggtgca
     3661 cgcagcacca ccaccgtgga tatgtcgcgt acttatctgg agtgggcaga acgcaacctg
     3721 cgtctgaatg gcctgaccgg gcgtgcgcat cgcctgattc aggccgattg cctggcgtgg
     3781 ctgcgtgagg caaatgaaca gttcgatctg atctttatcg atccgccaac cttctctaac
     3841 tcaaaacgaa tggaagatgc gtttgatgtt cagcgcgatc atctggcgct gatgaaagat
     3901 ttgaaacgtc tgctgcgtgc aggtgggacg atcatgttct cgaacaataa acgtggcttc
     3961 cgtatggatc tcgacggact ggcaaaactg ggactgaaag cacaagaaat tacgcaaaaa
     4021 acgctctccc aggatttcgc ccgtaaccgc cagatccaca actgctggct gattaccgca
     4081 gcctgaaagg aatagtaatg tcattaatca gtatgcatgg cgcatggctg tcgttcagcg
     4141 acgcgccgct tctcgataac gcagaactgc atatcgaaga taacgaacgt gtttgtctgg
     4201 taggccgtaa cggcgcgggc aaatcgacgt taatgaagat cctcaaccgt gaacaggggc
     4261 tggatgacgg gcgcattatt tacgagcaag atttgatcgt ggctcgtctg caacaggatc
     4321 cgccgcgtaa cgttgagggt agcgtttatg atttcgttgc cgaaggcatt gaagaacaag
     4381 cggaatatct gaaacgctat cacgatattt cgcgcctggt gatgaacgac ccgagcgaga
     4441 aaaatctcaa cgaactggcg aaggttcagg aacagctgga tcaccacaac ctgtggcagc
     4501 tggaaaaccg catcaacgaa gtgctggcgc aactggggtt agatcctaac gttgcactgt
     4561 cgtcgctttc cggcggctgg ttgcgtaaag cggcattagg acgcgcgctg gtgagtaatc
     4621 cgcgcgtgct gttgcttgat gaaccgacta accacctgga tatcgaaacc atcgactggc
     4681 tggaagggtt ccttaaaacc ttcaatggga cgattatttt catctcccac gaccgttcgt
     4741 ttatccgcaa tatggcgacg cgcattgtcg atctcgatcg cggcaagctg gtgacctatc
     4801 cagggaatta cgaccagtac ctgctggaaa aagaagaagc cctgcgcgtg gaagaacttc
     4861 aaaatgccga gttcgatcgc aaactggcgc aggaagaggt gtggatccgc caggggatca
     4921 aagcacgccg tacccgtaat gaaggccgcg tacgcgccct gaaagcaatg cgtcgtgagc
     4981 gtggcgaacg tcgcgaagtg atgggtaccg caaagatgca ggtggaagag gccagccgct
     5041 ccggtaagat cgttttcgaa atggaagacg tttgctacca ggttgacggt aagcaactgg
     5101 tgaaagattt ttcggcccag gttctacgtg gcgacaaaat tgccctgatt ggtccgaatg
     5161 ggtgcggcaa aaccacgctg ctgaaactaa tgctcggtca gcttcaagcg gacagcgggc
     5221 gtattcacgt tggcaccaaa ctggaagtgg cttatttcga tcagcaccgc gcggaactgg
     5281 atcccgataa aacggtgatg gataaccttg ccgaaggtaa gcaagaggtg atggttaacg
     5341 gcaagccacg ccacgtattg ggctatttgc aggactttct gttccatccg aaacgggcga
     5401 tgacgccggt acgtgcgctt tctggcggtg agcggaaccg cttgctgctg gcgcgtttgt
     5461 tcctcaaacc aagcaactta ttgattcttg acgaaccgac caacgatctt gatgtcgaaa
     5521 cgctggaact gctggaagaa ctgatcgaca gctatcaggg cacggtattg ctggttagcc
     5581 acgatcgtca gtttgtcgat aacaccgtta cagaatgttg gatcttcgaa ggcggcggta
     5641 aaattggtcg ttatgtcggc ggttatcatg atgcccgtgg tcagcaagag cagtatgtgg
     5701 cgctcaaaca gcctgcggtg aaaaaaaccg aagaagccgc cgcggcaaaa gcagaaactg
     5761 taaaacgcag cagtagcaaa ctaagctata aattgcagcg cgaactggag cagctaccgc
     5821 aattgctcga agatctggag gcgaagctgg aagccctaca gacgcaggtg gcggatgctt
     5881 ccttcttcag tcagccacat gagcagacac aaaaagtgct cgccgatatg gctgctgcag
     5941 agcaggagct ggagcaagcc tttgaacgct gggagtatct tgaagcgtta aaaaatggtg
     6001 gctgatcgca aaatagtcga tttaaacaga gacagctgac aacggtaaaa ttgcctgatg
     6061 aactgggttt atcaggcatg aaaaccaaca ctttgttagt catctgtaaa atagcgcatc
     6121 attaaggagt accaatgtgc gaacatcatc atgccgcgaa gcacatcctg tgctcgcagt
     6181 gtgacatgct ggtggcgtta ccgcgccttg agcatggtca gaaagcggca tgtccccggt
     6241 gtggcacaac gttaaccgtg gcgtgggatg cccctcggca gcgtcccacc gcctatgcgt
     6301 tggctgcact gttcatgctg ttgctgtcca acttgtttcc ttttgtgaat atgaacgttg
     6361 caggagttac cagtgaaatt acattactgg aaattcccgg cgtgcttttt tctgaggact
     6421 acgccagcct cggcaccttt ttcctgttgt ttgtgcaact ggttcccgcg ttttgtctga
     6481 taaccattct gttactggtg aatcgcgcgg aattaccggt ccgtttaaaa gagcaactgg
     6541 cacgggtgct ttttcaactc aaaacctggg gaatggcgga gattttcctc gctggtgtgc
     6601 tggtcagttt cgttaaactg atggcttacg gcagcattgg ggtaggcagc agctttctcc
     6661 cctggtgttt attttgtgtc ctgcaactgc gcgcttttca gtgcgttgat cgtcgctggt
     6721 tatgggacga catcgccccg atgccagaac tgcgccagcc gctaaaacca ggcgtcacgg
     6781 ggatacgtca ggggctgcgt tcgtgctcct gttgtacggc aatccttcct gctgatgaac
     6841 ccgtgtgccc gcgttgtagt accaaagggt acgttcggcg tagaaacagc ctgcagtgga
     6901 cactcgcgct gcttgtaacg tccatcatgc tgtatcttcc ggctaatatt ttgcccatca
     6961 tggtgacgga tttattaggc tcgaagatgc cgtcgacgat tctcgctggg gtcattctgt
     7021 tatggagcga aggctcttat cccgtcgctg cggtgatctt tctggccagt attatggtgc
     7081 caacgttaaa gatgatcgcc atcgcgtggc tgtgttggga tgccaaaggg catggcaagc
     7141 gcgacagtga aagaatgcat ttgatttatg aagttgttga gtttgtaggc cgctggtcga
     7201 tgattgacgt tttcgttatc gcggtgctct cggcgctggt gcgtatggga ggtttaatga
     7261 gtatttatcc ggcaatgggt gcattaatgt ttgctttagt cgtcataatg acaatgtttt
     7321 ctgctatgac gtttgacccg cgtttgtcgt gggatcgtca acctgaatca gagcatgagg
     7381 agtcctgaca gtatggaatc taataatggg gaagccaaaa tccagaaagt gaagaactgg
     7441 tctcccgtgt ggatatttcc tatcgtcacg gcgctcattg gggcctgggt tcttttttat
     7501 cattacagcc atcagggacc ggaagtgacc ctgatcaccg cgaatgcgga aggaattgaa
     7561 ggtggcaaaa ccaccattaa aagccgtagc gttgacgtcg gcgtggttga aagcgccaca
     7621 ctggctgatg atttgacgca cgttgaaatc aaagcgcggc tgaattccgg tatggaaaaa
     7681 ttgctgcata aagacaccgt tttttgggtg gtgaaaccgc agattggtcg cgaagggatt
     7741 agcggcctgg gaacgctgct gtctggagtt tatatcgaac tgcagccagg cgcgaaaggc
     7801 agcaaaatgg ataaatacga tttgctggac tcgccaccgt tggccccgcc tgatgcgaaa
     7861 ggtatccgtg tgattctcga tagcaaaaaa gccgggcagc tctcgccagg agatccggtg
     7921 ctgttccgtg gctatcgggt aggttcggtt gaaaccagca ccttcgatac acaaaaacgc
     7981 aatatcagct atcaactgtt catcaatgca ccttatgacc gactggtgac caacaatgtt
     8041 cgcttctgga aagatagtgg cattgcggtt gatctgacgt cagcagggat gcgtgtggag
     8101 atgggctcat tgacaacgct gctgagtggc ggtgtcagct ttgatgtgcc ggaaggtctg
     8161 gatttagggc agccagtggc accgaaaaca gctttcgttt tgtatgatga tcagaagagc
     8221 attcaggatt cgttgtacac cgatcacatt gattatctga tgttctttaa agattcggta
     8281 cgcggtctgc aaccgggagc tccggtagag ttccggggta ttcgcctggg taccgtaagc
     8341 aaagtgccat tctttgcgcc gaatatgcgt cagacattta acgatgatta ccgtattccg
     8401 gtactgattc gtatcgagcc agagcggctg aaaatgcagc ttggcgaaaa tgcggatgtt
     8461 gttgagcacc ttggcgaatt gttgaaacgt ggtttacgcg gatcgctgaa aaccggaaac
     8521 ctggtcactg gtgcactgta tgttgatctc gatttctatc caaatacgcc tgcaataacc
     8581 ggtattcgtg aatttaatgg ttatcagatt atcccgaccg ttagcggcgg cctggcgcaa
     8641 atccagcaac gactgatgga agcgttggat aagatcaaca aactgccatt gaatccgatg
     8701 attgaacagg caaccagtac gctttctgaa agtcagcgca caatgaaaaa cctgcaaacg
     8761 acgctggata gcatgaacaa gatcctcgct agccagtcga tgcagcagtt gccgacggat
     8821 atgcagtcaa cgttgcgtga attgaatcgc agcatgcagg gcttccagcc tggctccgca
     8881 gcctacaaca agatggtggc ggatatgcag cgccttgatc aggtgttgcg agaactgcaa
     8941 ccggtgctga aaacgctcaa tgagaagagt aacgcgctgg tatttgaagc gaaggacaaa
     9001 aaagatccag agccgaagag ggcgaaacaa tgaaaaagtg gctagtgacg attgcagcac
     9061 tgtggctggc cggatgcagc tccggcgaaa ttaataaaaa ctattaccag ttacctgtgg
     9121 tgcagagcgg tacacaaagt accgccagcc agggcaatcg tctgttatgg gtagagcagg
     9181 tcactgttcc tgactatctg gcggggaatg gtgtggttta tcaaaccagt gatgtgaagt
     9241 atgtgattgc caacaacaac ttgtgggcca gcccgttgga tcaacagttg cgcaacaccc
     9301 tggttgccaa cctgagtacg caactgcccg gctgggtggt tgcctcccag cctctgggaa
     9361 gcgcccagga cacgctcaat gttaccgtaa cggagtttaa cggtcgctat gatggcaagg
     9421 tcattgtcag tggtgagtgg ctgttgaacc accagggaca actgatcaaa cgtccgttcc
     9481 gtctggaagg agtgcaaact caggatggtt acgatgagat ggttaaagta ctggccagtg
     9541 tctggagtca ggaagccgct tctattgcgc aagagataaa gcgtctacct taattataaa
     9601 gatttgtaaa tataaccgtc tccggtatgt tgcctgaggc ggtttttttg tttctaacgt
     9661 gcggaaaaat ttgttcctct tcacattttt tgtacaaccg acatgcccgt gtagctcaca
     9721 aatatgacag tggcgtgaat tttgcgcatt gacggcagtt atgattcgcg gtattgctta
     9781 actgtgattg cacatttagt aatcactgtt ttcttttcca ccagaaacca gtatgaggga
     9841 aacgaggcat gaagagacaa aaacgagatc gcctggaacg ggcacatcaa cgtggttatc
     9901 aggccggcat cgccggacgc tcaaaagaaa tgtgtcccta tcagacgctg aatcaaaggt
     9961 cacaatggct gggaggctgg cgagaagcca tggcggacag ggtagtaatg gcctgattct
    10021 gtctctttaa aaagaaacct ccgcattgcg gaggtttcgc cttttgatac tctgtctgat
    10081 tataatcaga aggcagacgt atcctggaac agaccgactt tcaggtcgct ggcggtatag
    10141 ataagacgac catcaaccag cacttcgcca tccgccaggc ccataatcag acgacggtta
    10201 acaatgcgtt taaagtgaat acggtaggtc acttttttcg ctgtcggcag tacctgacca
    10261 gtgaatttca cttcgccaac gcccagcgcg cggcctttac cttcgccgcc cagccagccg
    10321 aggtagaacc ctaccagctg ccacattgcg tccaggccca ggcatcccgg cataaccgga
    10381 tcgccaataa agtggcatcc gaagaaccac agatccggat tgatatccag ttctgcttca
    10441 acataccctt tgtcgaagtt accacccgtt tcggtcattt tgaccacacg gtccatcatc
    10501 agcatgttcg gtgctggcaa ttgcgggcct ttagcgccaa acagttcacc gcgaccagag
    10561 gcaagaaggt cttcttttgt ataggattcg cgtttatcta ccatgttctc tgtaagcctt
    10621 attttattga agcacgcagg atagctaaca cgtgtacgct gaacaagtcc gatcagttcg
    10681 gaataaacca gttcagccaa cgtaatggcc atggaaaacg gtgacgtcct tcctgttgcg
    10741 atgcttgcgc gatacgttcc tggatggttt gcatcagcgt cgtttggcct tcgccatccc
    10801 acaccagatt taataataac ggcagtgcgt cagtcacatc gtctactgcc cagatggtga
    10861 atttgccttc ttctaccgct ttcaccagtt cactgtgaag acttaaatgg cgaacgttag
    10921 ctgtggggat aatgacacct tgtttcccgg ttaactcacg ttgctggcaa atagcaaaga
    10981 agccttcgat tttctcattt aaaccaccga ccggctgggc gcgaccgaac tgatcgactg
    11041 aacctgtgat agcgatactc tgattcaccg gcacatcggc gagggcgctt atcagggcgc
    11101 agagttcagc catcgaggca ctatctccat caacttcact gtatgactgc tcaaatgtca
    11161 gcgatgctga gaaggggatc tgttgctcaa gctgtagttc cgacatcagg aacgcttgca
    11221 tgatcatcat ccctttcgca tggatattgc cgccaagctc cgctttgcgt tcgatgtcgg
    11281 tgaattcacc atcgccaata tgcacaacgc agctaatgcg agaaggttcg ccaaaagcgc
    11341 gtggatgacc cggaaattca atgaccgaaa gggcgttaat ttgcccgatg cgttcgcctt
    11401 cggtttcaat caggatttgc tcctgaagga tctcatcctg catacgttca gcgaggaaac
    11461 cttcgcgcca ttcacgctgc tgcagcatta agtttagctg ctcgccggag aaggtgtcgc
    11521 catcacacag ggaggcgacc tctttacact ggcggaggat ccactgcggg ctaagcggaa
    11581 gtgtttcttg ttcaccggtg tagcgtgctg cttcgcggat aagtaccggc caggcatccg
    11641 ctcccggtgc aggtaagtga ttatgtctgg cggtaaatgt cacccagcga caccactggg
    11701 ttactgactc tgcatcgaca atctgcagag tatcttcaaa ttcgctataa atagcctgct
    11761 ctgaaagctc tggctccatc tcctggaaat cagccaatga ttcgcgttcg cctaccagaa
    11821 tgactttcag cttcaatggc atcgaaggca cagagacggg gagagggcgc gactcatcaa
    11881 acgcaaccca gtcaaaacgc tcgcggttaa cgatattttt cagccgcatc cacagcagag
    11941 gttgcgccag cagtgtacgc aaagagatga tgagaatacc gccatttgcc tgatgcacca
    12001 gaccaggctg cagggtaatg tcgccattaa actggcgcag gcagccaaag agttgctccg
    12061 cttctaccca gtcggcagcg acaacttgcg ttaaagtcgc aaaattatca tctgcactca
    12121 ctgcgtgacg taagcggatg gagtggccgg aaacctcata gtgaccgccc accagttggc
    12181 ctgcatcgct ttgtagcgta cgcgcggcat tggcaataag attcagatac tcagactctt
    12241 ccggggcctt cgccagcata aaagaggagg atgctcgcgt atgcagcaac tgctccagtg
    12301 cgaattgcag tcgcggttga gtatcactga ataaaggatc gttttcgtca atcaaatgtg
    12361 gctgagcaaa tatttcctga tagctatcgg tatcaggaac caggtcacgc catgcaagtt
    12421 tcgtaatggt caaagttgat gttttttagt ctgttgtcaa agccgcgatt ataccgtaac
    12481 cggcactaca gcacacgtag aaagcaccga caatactcct ggcatgggcg ttaaagctca
    12541 caggatggag attcttttct tcactggcct aaaaagctga tattctgtaa agagttacac
    12601 ggtaacattg agatcgctat gaaatatcaa caacttgaaa atcttgaaag cggttggaaa
    12661 tggaagtatc tggtgaagaa gcatcgcgaa ggggagttaa tcacccgtta catagaagcc
    12721 agtgccgccc aggaagccgt cgatgtgttg ctctcgctcg aaaatgaacc cgtgctggta
    12781 aatggctgga ttgacaagca tatgaatccg gaactggtca atcgcatgaa gcaaaccatt
    12841 cgggcaagac gaaagcgcca ttttaatgcg gaacaccagc atacgcgcaa aaaatcgatc
    12901 gatctggaat ttattgtctg gcaacgtctg gctggtcttg cgcagcgtcg cggtaaaacg
    12961 ctttctgaaa cgattgttca gctgattgaa gatgcggaaa acaaagagaa atacgcgaat
    13021 aaaatgtctt ctctgaagca ggatctgcag gcattgctgg gtaaggaata actgacgaaa
    13081 gtcagttcaa tttactaaag gcaaaaaaaa ccccgcagca gcggggtttt tctaccagac
    13141 gagaacttaa gcttgcggct gagttacaac gtctttgata cctttaactt cgatctctac
    13201 gcgacgatcc ggagccaagc agtcgatcag tgcagcacgc tgtttcacgt tgtcacaggt
    13261 gttgccagta accgggttgg attcgcccat accacgtgcg gagatcttgt ctgccgggat
    13321 acctttggag atcaggtaat caacaacaga ctgagcacgg cgctcggaca gaccctggtt
    13381 gtaagcgtca gaaccgatgc ggtcggtgta acccagaaca actacggaac cgtctttcgg
    13441 atccaggttg ctcagctggc tgtacagctg atccagagca gcctgacctt ccggtttcag
    13501 ggttgctttg ttgaagttga acagaacgtc agacttcaga gtgaagtgct tggtctgtac
    13561 ttccggtgcc ggagctggag ccggagcaac tactggagct gcttcgccct gaccgaaacg
    13621 gtaggaaaca cccaggctca gcatgccgtt gtccggacga gtgccgatgg tgtgtgcgtc
    13681 accgatgttg ttggtccact ggtattccag acgggtagcg atttcaggag tgatcgcgta
    13741 ctcaacaccg ccagcgaaga ccggagaaac gccggtgtcg tggtttttac cataaacgtt
    13801 ggatttagtg tctgcacgcc ataccatacc acccagacga gtgtagatgt ccaggtcgtc
    13861 agtgattggg taacccagtt tagcggtcag ttgaacgccc tgagctttgt atgcaccgtt
    13921 ttcaacgctg cctttgtacg gcatacgacc taaccagtcg taacccattt caaagccaac
    13981 atacgggtta acctggtaac caccaaaagc accagcgccc agttggtttt catgggtcgg
    14041 gccattgttg ttgatgaaac cagtgtcatg gtactgggac cagcccagtt tagcaccagt
    14101 gtaccaggtg ttatctttcg gagcggcctg cgctacggta gcgaaaccag ccagtgccac
    14161 tgcaatcgcg atagctgtct ttttcatttt ttgcgcctcg ttatcatcca aaatacgcca
    14221 tgaatatctc caacgagata acacggttaa atccttcacc gggggatctg ctcaatatta
    14281 actctaccga tatcttcggc ttatgccgag cacccctggc gatgtaaagt ctacaacgta
    14341 gttgaaaact tacaagtgtg aactccgtca ggcatatgaa aaaaaagtct tgtataaggt
    14401 atgtttaatc ttttttgtca gcgacaattt acagaagaaa atcgcggaaa ccgcttcaga
    14461 caagcctccg caaggaaaat tagtcacgac tgaaagcatt ggctgggcga caaaaaaagt
    14521 tccaggatta atcctaaatt tacttaatga tacaaattag agtgaatttt tagcccggaa
    14581 agttgtctcg tggcgtgaga ggatgcgctt accggacgca taataaaccc catagcgtta
    14641 ccttcatttg ccgcatcaac aagttcagca tgctcttctt cagtcaaatc atctgccaac
    14701 caaccgatca ccacactgta attgcccgtg cgtaaagcgc gaaccattga ctccacagtg
    14761 tggcaagggg agagctggct aatctgcatt actttcgtta agggtagccc agatgcctga
    14821 acccattccc gactcagttt ttgttgcggt gttaaccaga gttgccagcg cgattgctga
    14881 ccgagttgct gtaacaatgg caacagtaga agttgcgtca tcatgggctg atcttcgcga
    14941 tagacaactt cactgataag cccggctgta gtgttttccg tagagacacg cgcaatttta
    15001 cttgctgcgg atgagaacga cgaagaacga tgtgcatagc ctgaagtgta cataatcaat
    15061 ccagcccctg tgagttactg tatggatgta cagtacatcc agtgacaaca aagatcaacc
    15121 ctattttcgg aaagagcctc gcaaattttg tcgttggtga cgggaaaaca taaattaatc
    15181 ttgcccctta agaataagtt gcctattttc gtagttaacg gatccgttaa tgtgaatcat
    15241 tcttttatgt tatgatttta aaaggaattt tatgaaaagc ctctcctata agcggatcta
    15301 taaatcacaa gaatacctgg caacgttggg cacaattgaa taccgatcat tgtttggcag
    15361 ttacagcctg accgttgacg acacggtgtt tgcgatggtt tctgatggtg agttgtatct
    15421 tcgggcttgt gagcaaagtg cacagtactg tgtaaaacat ccgcctgtct ggctgacata
    15481 taaaaagtgt ggccgatccg ttaccctcaa ttactatcgg gttgatgaaa gtctatggcg
    15541 aaatcaactg aagctggtgc gtctgtcgaa atattctctc gatgcagcgc tgaaagagaa
    15601 aagcacgcgc aatacccggg aaagactgaa agatttgccc aatatgtctt ttcatctgga
    15661 agcgattctc ggggaggtgg ggattaagga tgtacgggcg ttacgtatac ttggggcaaa
    15721 aatgtgttgg ttgcgactgc ggcagcaaaa cagtctggtg acagaaaaga ttctgtttat
    15781 gcttgaaggt gccattatcg gcattcatga agctgcgctc ccggtggcac gccgccagga
    15841 gcttgcagaa tgggctgact ctcttacgcc gaaacaggag tttcctgcgg aacttgagta
    15901 atctggcgtt gcagacgacc aatctcaggc agtaatgcaa tcaataatcc aacttgttgt
    15961 acgaccagag gttctttgct gtctgcccgt ggttcaagtt gctgcatccg ctgtttcagg
    16021 ctagctaatg cctcattgac gcgttcttca tcagcaggtt gatgatgtaa cgcgtcatca
    16081 acatagcaaa ctgcctcatc aagaaacgcc agaatttcag gattagttaa ctgctcccgg
    16141 tgagcaccga gggctgagat atagctggta aacgtatggt taaggcacag caaccgaaac
    16201 gcggcttcgc gaatttgcgg ggtaacgttc ggttcgctgg acatatttga taccaccgac
    16261 gccagttcag catcacggtt gtgtgcatcg cggcgggcaa tacgatacgc cagacggtta
    16321 tcacgcccct gatggtattg ctccagtatg gcatcgagat accggcagtt ggcctctgtg
    16381 gcgcgttcga gcattcgcgg cagattgcga aactgccagt caggccagat gtagctcact
    16441 gccgcccacg caatggcaca accaatcagc gtatcgatta cgcgaggtaa cgctacttca
    16501 aaaccttcac ccagcaagtt aaaacacagt agcaccaaaa gtgtgatgaa catcgttgca
    16561 tgggcatatt gcacgttacg gaaggcaaaa aagagcacgc cggtaataac cagtagcacc
    16621 agctgccctt ccagtgacgg tacaaaccac agcacaggaa tgccaatggc gataccgacc
    16681 agcgtaccaa taatccttaa cttcaggcgg tggcgcgtgg cgttatagtt tggctggcag
    16741 acaaacaaac ttgtcagcaa gatccaatac ccgtgatgca ttccggttat ttgaatgatg
    16801 gcgtagccga agcacaacac cagcgacatt cttatcgcat gacggaagag ggcggattcc
    16861 ggcgtgaagt gacggctaag acgcagccag atatcactca acccgtgcgg gctgtcatca
    16921 gcgagctcat tttcgtcatt attatggggt agtgcctggg cctgttctga ttcaattgtt
    16981 gccagttggg catcaatggc gcgtaaattg ttcagcaaaa atcccagtgt tttgagtaaa
    17041 tcggcgggtg cgccgttatc gcgcatccgc tccagcgcag catcaatatg cgtaaaagcg
    17101 cgctcaaaat gcggatcatg ttgataaggc tgacgcaaca aaatacagcg tgacagttgc
    17161 tggcacgcct ggccctgcat cgacatcagc cgctgaaaac ggaacagcac gtcgctgtgg
    17221 cgaaaatgtt cacgcaatgt ttgatactga atatgagaag agctggcacg ctcgtgaata
    17281 tcctgtgcga caaagtaata atgcagcgtg cgacgcgttc cccgttgacc acgatcgcca
    17341 cgtaagcggg tcagcagcga gagtttcgtc tgattcaatg tcgccatcag cagaccgttg
    17401 gcgagagcca aatcgtacag cggtgcctgg ctttcatctt caatatcagg atcaaacatg
    17461 cgcgacttga gctcaagata acgcgccagt tgttcatagc aacgcgccag gttgtcctgc
    17521 agcgggcgga ccgggaacag cagatgacca ataagtgtca ggacgttgta ccagacggca
    17581 ccggccagca gatacatcgg ctgctgatac cagtgctcat acagtgatgt tcccaacata
    17641 gtgtaaatgg cgatcagcaa tgcaccgaag gcaattgttg catagcgttg acccagaccg
    17701 ccgagcaaaa tgaagccgct ggtagagagc gttaagccaa tcgcaaatag ccagggccag
    17761 ggaaacagca attctactga ggccgaggcg ataaaaaagc agaacagcgt aatgatgagg
    17821 ttacgcaaac gtcccgccag tcggtcatcg agatcggtca gcgccgctgc caccatcccc
    17881 agcgttagcg gaatcgtcag ttttacatca cccagccacc acggaaacgc tgtggttcca
    17941 caaagcgcaa taaaaatacg cgcgtaatac agccaggcgc tgttccaggt atagcgtttg
    18001 agcaaaggac ttagcataaa ggccatatta gtggattatt caaaacgacg acgtgcattg
    18061 gcttcacgcg cagcttgcgc tgtttctacc gataccacgc gacgaccaac cggccatagt
    18121 gcaatagcgg caattttaaa gttcgcaatg ccgacaggaa tgccaatgat tgaaatacat
    18181 tgtgcgatgc ccgttgcaat gtgcatcagg cataaccacc agccaaagaa aatcagccag
    18241 aaaatattca ataccgtacc gccagtattc agcagcacat ttttgccagc cgggttcagt
    18301 tcatcgacat gaatagcttc attgccataa ggcaccagag acagtttagt gatctcccag
    18361 caggatcgtg tcagcggtaa ggtaaaaatc agcacaatac tgaccagagt cgccaacagc
    18421 cagcccagag tggtggcaaa tccgccaagc acaaagttca gaatgttcaa aacggtacgc
    18481 ataaaacctc gctttactgt ggttttcagt aatgggcggc aattgtaacg tttttttggg
    18541 ctggagcacg ttttctctga cggttacact gataagaaat aatttcgtgt ggatctacag
    18601 agtcatggaa ctgaaagcga caacgcttgg aaaacgtctg gcacagcacc cttacgatcg
    18661 ggcggtgatc ctcaatgccg ggattaaagt ctccggcgat cgccacgaat accttattcc
    18721 tttcaatcaa ttactggcga ttcactgtaa gcgcggtctg gtatggggcg agctggaatt
    18781 tgtactgccg gacgaaaaag tggtgcgtct gcacggcacc gaatggggcg agacgcagcg
    18841 tttttaccat catcttgatg ctcactggcg gcggtggagt ggcgagatga gcgaaattgc
    18901 gtctggtgtt ttacgccagc aactggattt gattgccacg cgcactggag aaaataaatg
    18961 gctgacgcgt gagcaaacct ctggcgttca gcaacaaatc cgccaggctt tgtcggcgtt
    19021 gccgttgccg gttaaccgac tggaagaatt cgataactgc cgtgaggcgt ggcgtaaatg
    19081 tcaggcctgg ttgaaagata ttgaaagcgc tcggttgcag cataaccagg cgtataccga
    19141 agccatgctt accgagtatg cggatttttt ccgccaggtc gagtcttcac cgctgaatcc
    19201 ggcgcaggcc cgggcagtcg ttaatggcga gcattctctg ttagtgctgg caggtgcagg
    19261 aagcggaaaa acgtcggtgc tggtggcccg tgcaggctgg ttgctggcgc gtggtgaagc
    19321 gtcccctgag caaattttat tgctggcgtt tggtcgcaaa gccgctgaag agatggacga
    19381 gcgtattcgc gaacggctac ataccgaaga cattaccgca cgcacgtttc atgcgctggc
    19441 gctgcatatt attcagcagg gcagcaaaaa agttccgata gtcagcaaac tggaaaatga
    19501 taccgctgcc cgtcatgaac tctttattgc tgagtggcgc aagcaatgca gcgaaaagaa
    19561 agcgcaggcg aagggctggc ggcaatggct gacggaagaa atgcagtggt cagtgccaga
    19621 aggtaacttc tgggatgatg aaaaattaca gcgtcgtctt gcctcacgcc tcgatcgttg
    19681 ggtaagtctg atgcggatgc acggtggtgc acaggcagaa atgattgcca gtgcacccga
    19741 agagattcgc gatctgttca gtaaacgtat caagttgatg gccccgttat taaaagcctg
    19801 gaaaggtgcg ctgaaggcag aaaacgctgt cgatttttcg ggccttattc atcaggcgat
    19861 tgtgattctg gagaaaggcc gctttatcag cccgtggaag catattctgg ttgatgaatt
    19921 tcaggatatc tcgccgcagc gcgcagcgtt gttagcggca ttacgcaagc aaaacagtca
    19981 gacgacgttg ttcgccgttg gtgatgactg gcaggcgatt taccgattca gcggtgcgca
    20041 aatgtcgctc accaccgctt tccatgaaaa ctttggtgaa ggcgatcgct gtgatttaga
    20101 cacgacttac cgttttaaca gtcgtatcgg tgaggtggca aaccggttta ttcagcagaa
    20161 cccaggccag ctgaaaaagc cgctaaacag cttaaccaat ggagacaaaa aagccgtcac
    20221 gttattggat gagagtcaac ttgacgcttt gctggataag ctctctggtt atgccaaacc
    20281 ggaagagcgc attctgatcc tggcgcgtta ccatcacatg aggcctgcca gcctggaaaa
    20341 agcggcaaca cgctggccga agttgcaaat cgactttatg accattcatg ccagcaaagg
    20401 gcaacaggcg gattacgtca tcatcgttgg cttgcaggag gggagtgatg gttttccggc
    20461 tgcggcgcgg gagtcgatta tggaagaggc gctactgcca ccggttgagg atttcccgga
    20521 cgctgaagaa cggcggttaa tgtacgtggc gctgacccgg gcacgccatc gggtatgggc
    20581 actgtttaac aaagagaatc cctctccctt tgtggaaata ctgaaaaatc tggatgtgcc
    20641 ggtagcgaga aaaccgtaag aaacaggtgg cgtttgccac ctgtgcaata ttacttcaga
    20701 cggtccgcga gataacgctg ataatcgggg atcagaatat cgaccgcgtc gttgaaatgc
    20761 ggcgactgga ttatgaagtc tgccgttgcc acgttggtgg cgaccggaat gttccatacc
    20821 gtcgccagac gcaacaaggc tttcacgtca ggatcgtgcg gcacggcatt tagtgggtcc
    20881 cagaagaaaa tcaatacatc aattttccct tctgagatca atgcgccaac ctgctggtca
    20941 ccccccattg ggccactcaa catcgcgttg acgttcatgc cggtcgcgcg ggaaattaag
    21001 ttaccggtag tgcctgttgc atacagtacg tgttgttcca gtaacggttg atgccgttcc
    21061 acccagctca tcagcatttg tttgcagtga tcgtgtgcca ccagcgcaat atgtttccgc
    21121 gcaggtaaag tgcgagtcgt cagttccata atgtacatcc gtagttaact ttcctacaga
    21181 ttactgtaag cacttatcgc tgcaagataa agaccgaaaa agcctgcgca caggcacaaa
    21241 aatctcagga agatggttgt ttttccgccc attgtaggaa agtatttcgc gtttgtgggt
    21301 cagccagttt aaaccaatac ttcagccgtt gttctgtgag cacctgagac tgcggtggaa
    21361 tagcatccag ctcggaaaca cccgaaagca gcgtactgtc atctgccatc atcgtggcaa
    21421 attgcggtag tgaagcgcgt ttcgcggatt tgttatagcg ttcaacctcg acctcataat
    21481 caatcgtttt tgccgttgag gtaatggcga gaatatccag ctttaccgga atcggcgtcg
    21541 catcgccatc caacaattca aggcgcggcg cggcatcaaa atggttagct tcccgctcat
    21601 tctccaggcg aggcaggcga aaattgacct ggttgatgag ctgggtatta aaactgacca
    21661 ccagcggtgg ggagatatac agccgttctt cactattgga aagatgaatc gtcttctcaa
    21721 cgcgaaacac taactgatgc ggtccattat ccagttcaat gctgtccgca ccgcgcagca
    21781 gagagctgga aaccttcttg ccgtcgagta ccagaagatc gacatcggtt gaaagccgca
    21841 gcgtggtggc aaaaacggat accggcaaac ataatgcaat caaggtggtc acgatgccgg
    21901 ttttcatagg tagctcctgt caaaagaccg cccgaataat aatgtgtcaa aatttttctt
    21961 taatcatact tactaacata tagacatatt tcccgctttt gctctcattc attcgtatta
    22021 gctgcatggt tggcatgtcg gctttgtcgt acactttgtt aaacagtctg gaggaaataa
    22081 tgaaagaaac cgatattgca ggcattttaa cgtccactca caccattgcg ctggtggggg
    22141 caagcgacaa acccgatcgt cccagctatc gcgtgatgaa atatctactc gaccaggggt
    22201 atcacgtcat tccggtttcg ccaaaagttg ccggcaaaac gctgctgggc cagcaggggt
    22261 atggcacgct ggcagacgtt ccggaaaagg tcgatatggt cgatgttttc cgtaattcag
    22321 aagcggcgtg gggcgtggca caggaagcta ttgccattgg cgcgaaaacc ctctggatgc
    22381 aactgggggt gattaatgag caagcggcag tactggcacg ggatgccggg ttgaatgtgg
    22441 tgatggatcg ctgcccggct attgagatcc ctcgcctggg cctggccaaa taaaaaatcc
    22501 ccggaaggca aaaaccttcc ggggattcgt tcagggatca gttacgcaga cgcggggcct
    22561 ggagttgttt gcggatggtc tgcgccagct catccatgga tggctgttca ggatgttcat
    22621 cctgcaattc gctacttagc tgggcttcag ccaggtaggt atgaaccggt aggccgttat
    22681 cgtcctccat cactacgtga taccacggag cggcgcgaag ctcgtcattt accgccagct
    22741 catcaggcga cggttcagag agcgaataaa ccgggtcgat atccacgacc actccgagat
    22801 aacctaacag ggaatggcgg acctgctggc cgataccgaa tttgctggca atcatagtca
    22861 cctcccggga aatctttacc tttgatataa gggtaaaatt ccacatttca agttacatga
    22921 cgcgacaggc aaaccctttc agatatagcc cttccggata ggtagcgatc accggatgat
    22981 cggctgcctg acggaactgc tctataaatt gtacatcacg gccggcatca attgcggcat
    23041 ccgcgatgat tttctgaaat aaatcgctgg tcatcagacc ggaacaggag aaagtcagga
    23101 gaataccgcc ttcattcagc aactgaatcg ccagcatgtt gatatcttta tagccacgac
    23161 acgcgcccat caactggctt ttattctcaa caaacttcgg cgggtccatc acgataacgt
    23221 caaatttttc accgcgatcg cgataagtac gcagcaattt aaagacatca tcacggacaa
    23281 actcagcctt gctcagatcc agtttgttca gctcaacgtt ctgccgtgca atatccagcg
    23341 cttcctggga ggtatcaacg ctgacaacct ggctgcaacc gcccatcagt gccgatacgg
    23401 cgaaaccacc ggtataggag aaacagttca gcacacgttt attttcaacg tagcggcggg
    23461 tagccaggcg gctatcacgc tggtccaggt agtagcccgt tttgtgtccg tgctgaatat
    23521 ccaccagcag tttcattccg tgttcttcaa tcggcagcag ggcaggtggc aactcgccgg
    23581 tgacggggcc ctgggtcagc tccattcctt cttttttacg taccgcgacg tcgctgcgat
    23641 cgtaaatcga acattccggg tacagcgttt gcagggcact aattaatgcc gcgcgctgat
    23701 attctgcccc agcactcagc agttgcagca ccagaaaatt accgaaacga tcgatagtaa
    23761 tacccggcag gccatcagat tctccggcga ttaaacgata gctgtcgagg ccatcttttt
    23821 gcgccagcca gtcacgccat ttttgtgctt gttgcaaacg gcgggaaaaa aaagcaatgt
    23881 cgatagactc agacgggtca aacgtccaga cgcgcgcccg gatttgcgaa gctggcgaat
    23941 aagcgccgcg tgctaaccat tttccctgat gatcaacaat atcgatggtt tcaccgaggc
    24001 tggctttacc ttccatgcgg gcaacggccc cggaaaagac ccacggatgg cgacgaagta
    24061 atgatttttc gcgccctttg gctaacacta aacgtacact cataatttac ttttctgtcg
    24121 atgcaaagaa atgggcgtca ttgtccggag ttttccatgg atttgcaacg cgtccattaa
    24181 ggataaggag agcagcaatg tcgaaagtct gcataattgc ctgggtttac gggcgagttc
    24241 agggcgtagg atttcgctac accacacagt acgaagcgaa aagactgggt ttaaccgggt
    24301 acgccaaaaa tcttgacgat ggcagcgtgg aagtggttgc ttgcggtgaa gaagggcagg
    24361 tggaaaaatt aatgcagtgg ttaaagagtg gcggcccgcg ttctgcgcgt gtagaacggg
    24421 tgctcagcga gccgcatcat ccctcggggg aattaaccga ttttcgtatc cgttaaatac
    24481 attttaccgg ttttggcagg ccagcaattt tggtggcttg cttagccgga cctttcggaa
    24541 acagtcggta taaatagcgg ctgttacctt tttcttcgcc aaatttattc gccatcgctt
    24601 ttaccagcat acgaatcgcc ggagaagtat tgaattccag atagaaatca cgcacaaaac
    24661 gcaccacttc ccagtgttcc ggtgacagcg aaatcccttc gttctctgca atcaccacag
    24721 ccagcggctc actccactgg ctgctttctt tgagatagcc ttcggtatcc gtttctatct
    24781 ctttaccttc gaagatcagc ataattatta ctaccttaat cagactggcg gcagtgtaac
    24841 aaaaaacaaa gccccgcata aagcgaggct atgaaagtgt tagcgggtga gattaatcgc
    24901 ggctagcgaa gcccagaatg ctcagcaggc tgacgaagat gttgtacagc gaaacataca
    24961 ggctaaccgt ggcacgaata tagttcgttt caccgccatg aatgatgttg ctggtttcaa
    25021 acaaaatagc gccagaggag atcaaaatga agaccgcgct gatcgccaga tgcagagcag
    25081 gcagctgcag gaagatattc gcaaccatac caatcagcac caccacaata cccgccatca
    25141 gcataccacc gaggaacgac atatctttgc gggtggtcag cacatatgca gagcagcaga
    25201 agaacactaa cgccgttccg cccagtgcca tagcgattac gtcacccatt ccggcagaca
    25261 gataggtgtt cagaataggt ccgaggatat aacccagaaa accggtaaag gcgaatgcgg
    25321 agataatccc ggtcggctta ttcgccgttt tataggtcag gaacatcaaa ccatacatac
    25381 ccaccagcgt cagaatcaga cccggagatg gcagcatcag caccgtactg gcagttgcgg
    25441 taatcgccga aaaggccagc gtcaggctca gcagaaaata ggtattacgc agcaccttat
    25501 gggtgctaag cagtgatgta cggtcatgtg aagaactaac aatacgatcc attgagtcac
    25561 tctctatgac agatgtaatt aattaagcag cataatgata atgcgtaagg gcacccagaa
    25621 gttttaccca tctttacgca tttgatctgg aacaggttta acagcggatt atcaggtcat
    25681 taagcaaata taacgccctg agaatttcga caggcaaaag aaaaaggggt tagcatttag
    25741 ctaaccccct atcttatttg gcggaagcgc agagattcga actctggaac cctttcgggt
    25801 cgccggtttt caagaccgaa gaaaaacaaa tgaaaatcag tgtaatacgt gaatctgctg
    25861 aaaatttaat taaatttaac accaagaaaa atcaaggtgt taggaagttg ctcatagcta
    25921 gtataccaag tatttttgcc agaaaatttt ctgtattgcc tacttgctaa ccacgagcgg
    25981 cagatccagt gttggtgtga tttttgtttt acgatcgtaa atcaacacct gattttctgt
    26041 tttatgcccg ctgaaaattt gtttgtcgcg actgctgcct tcgtaatctg agatcccctt
    26101 ggcttttatg tcgtgaaaat tgcacccgaa cggaacgcca actttttgct cggctgagcg
    26161 tttagcctga ttccaccagt tgttcagcgt tttagctatg actttcccgc ctttggttgt
    26221 attgatcaca tattcgcatg tacaggaaga tacatttcgg gctaactgga tcgctgtacg
    26281 taatcgcggt gaccattcct tgatttgttt ggtgccggtt ttgttttgct caatgtagat
    26341 ccctttatcc ataatatcct gccatttcaa ctcaagcaca tcaccgagcc ttgccgcaca
    26401 gagataggaa atctccattg caatgcgtaa ctgtggaatt gcttccgcat atatcgctgc
    26461 atactgttca tcggtgatgt aaacagtgcg ggctttaaga gagaattttc tgactccttt
    26521 acatgggtta ttcttcacgt atccacgctc atatccccac ccgtatactc gactcaggct
    26581 tgccagttca tgatttgcct gggtcttgct ctcaagccct cgtttatcca tgaaaattct
    26641 tacctgctca gttttgacat tatcagcaag cacttttccg aataccatca gcaacgcctt
    26701 ctgatgttgt cgataatctt tttgggttcg gggggccagt tctgtaaatg caggggagtc
    26761 cataaacatg tgccataatt tagctacggt cattatgttg tggagttttg ctttttccag
    26821 ttcataattt tgccagactt tagctacgct ggttttccgc actcttccta gccctatagt
    26881 tcttgtactt ccttctggtt tccacacgta actgtaacca ttcgatctaa cccgcggtgg
    26941 cagtacatta tcttttttat tttttcttgg tcttcccatt gttcagtgcc tcaaaatcgg
    27001 gttcagcaga aaccagttca gatgtttttg gcatcgttgt cagtccgtgt ggaatatccc
    27061 tgcgtagaac tattggttcg tttttaggac cgattacaaa tgggatgccg tgcagtctta
    27121 actggtgttg ctgttttgtg tatcgctcgt atttcgtgat ctctttaatc tctgctggag
    27181 atagagttaa ttcgtacatg tggtcacgtg cctttacagc atgaccgccg ccactatagc
    27241 tggtgggcgg cgatatgggt tgaacattaa aaatcagcct gactcgggat cagtttttgc
    27301 cagatagctg aaacgtattt tgcctgataa cgggcgtcat caagtgcatt atggcgctca
    27361 ccttcgaatg gggtaacagt tctggcatcg aagtctatgg ctttccccag ctcaacgatt
    27421 gtgcgtacat cgcgatcgtt gtagtaacgc cacgggcagg ggatcccctg ccgttcgtat
    27481 gaacggcgca aaatcgtgtt gtcgaagttg gctccatttc cccagacctg aacaaaaaat
    27541 tcaccggagt tttcgtcgat aaattcccgc aattgtaaca gtgcatcatc taacgggatt
    27601 tcatcggtca taatggcaga ttgcgcttcg cgtgattgct taagccacca tttaatggtg
    27661 tcccgatcaa tgaccccgcc agcagtttcc agatcgatag tcttactaaa ttccggtccc
    27721 atatctccgg tttgcggatc gaaaaatatt gcacctattg agatgatcgg ggcatcagga
    27781 ttttttccca tggtttcaag gtcaatcatc agatggtgcc acaacctgct ggtggatgtg
    27841 ataacgtgat gaccgttcac cgtaattaag ggatctgccg tctcgccagt ttcactatcg
    27901 ctggcgtgat cctgagcgct gccagcattc tccttgtgtg gatgttcagc gccttccatt
    27961 tcctccggat catcttcctg aacttcaacc tgatactctt catcgaatgt ttcctggtat
    28021 gttgcgtcgc ccatcaccgc gccacaatca gggcagttgc cgccgccggt ctgaccgcag
    28081 gcggtgcaga tcttttccgg ttcctgttgc actactggtt caggttgttt cgtttctggc
    28141 tcgttttgta acgcatttgg gctgttttgt tccgctttct ggtcgttctg ttccgattct
    28201 tgctggttct ggtctacaga atcgcgggtt tcaatcccct ttacccattt cggatcattc
    28261 gggtcgctaa tccctgcaac aaattctccg cgagaggcag caagcaactt atcggcgtca
    28321 ggctggctga tactggctgc ctgcataatt ttgtttactt cgtcagcggt aacttttacc
    28381 aggtctggtt gtgcggtcgt gtcagatgca tcagtatttt gttgtgaacc tgtgtatgtg
    28441 ccgtttttgc gggcaaaata ttcttctttc gtgatttcag ttgccccggc agccagcgcc
    28501 ttatccagac cagaaagttt gtttgcgcga ccgtattttt cgccatcctt gtcggtgaag
    28561 aggaaataga acggcccctc acgctctaca gatggttcga cttccacttt gcattcggtt
    28621 ttttcgttgt ccggcattgc cgtttccact gcatcagctt ctggtactgg cgacgggaga
    28681 gtattagttg tgctctgatt tgttccttca tcttcaaaca cgccctttgt agtcaggtat
    28741 tcagtgatgt atttgttcag cgccacggga tctttgtgaa tgtcgatcgg acgttcacgg
    28801 acaaggccaa aaatagtctg acggtcatag ccaagtgctt caggctgttt gcgcattgat
    28861 gccgagatac gcttccagtc ttcgcggtcg ttgtcgataa cttcattttt tgcccagcga
    28921 tggatgctgc cgtcaatgtt tcccgcatcc acatcaccgg gccagagggc gcaggccagc
    28981 tcattgtcga gagttttcca tgtctgtttg tattcgcggc gaacggcaac agtgacagct
    29041 ccgttttttt cagcagcgtt ttcagtgttc tgttggttta ctctggaacg ggcgagatca
    29101 acaacagacg tgtattttcc ggtttccttg cgttcacctt cgcggcgttt tttccagatg
    29161 cgcatctctg cctgaatttc aggccattta gcgccaggct cacatttatg cttaacccac
    29221 ccgatggcat gcagtttaag ttcaggatac atggagttaa cttctggcat tttcataaac
    29281 gcttcaacga tatgtccgcc gaatgttgcc atgtcttcct gtagtaattc ctgtgcgcta
    29341 atcaccatat caacggtgat gttttcgcat gtgtcgaatt taaccaggac tgcgttctgt
    29401 acttcagggg acagcttgtc aaaattgacg ttcatcggat cggattcagg ttcgaccggt
    29461 acaaaggaag ctgactcctc atcccagcga ttttcctgca tatattcggt atcccaggaa
    29521 tcgagggcag ggcggggtat gcc
//
LOCUS       DAZGVQ010000052        29421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000052 DAZGVQ010000000
VERSION     DAZGVQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.052"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            108..1061
                     /gene="ybhD"
                     /locus_tag="ACHLAG_002171"
     CDS             108..1061
                     /gene="ybhD"
                     /locus_tag="ACHLAG_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0655404.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            complement(1102..2097)
                     /gene="pgl"
                     /locus_tag="ACHLAG_002172"
     CDS             complement(1102..2097)
                     /gene="pgl"
                     /locus_tag="ACHLAG_002172"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFP0655405.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2252..3070
                     /gene="ybhA"
                     /locus_tag="ACHLAG_002173"
     CDS             2252..3070
                     /gene="ybhA"
                     /locus_tag="ACHLAG_002173"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFP0655406.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(3071..4129)
                     /gene="modC"
                     /locus_tag="ACHLAG_002174"
     CDS             complement(3071..4129)
                     /gene="modC"
                     /locus_tag="ACHLAG_002174"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFP0655407.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4132..4821)
                     /gene="modB"
                     /locus_tag="ACHLAG_002175"
     CDS             complement(4132..4821)
                     /gene="modB"
                     /locus_tag="ACHLAG_002175"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFP0655408.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITIPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4821..5594)
                     /gene="modA"
                     /locus_tag="ACHLAG_002176"
     CDS             complement(4821..5594)
                     /gene="modA"
                     /locus_tag="ACHLAG_002176"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0655409.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLSGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5761..5910)
                     /gene="acrZ"
                     /locus_tag="ACHLAG_002177"
     CDS             complement(5761..5910)
                     /gene="acrZ"
                     /locus_tag="ACHLAG_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFP0655410.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            6039..6827
                     /gene="modE"
                     /locus_tag="ACHLAG_002178"
     CDS             6039..6827
                     /gene="modE"
                     /locus_tag="ACHLAG_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFP0655411.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6895..8367
                     /gene="modF"
                     /locus_tag="ACHLAG_002179"
     CDS             6895..8367
                     /gene="modF"
                     /locus_tag="ACHLAG_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFP0655412.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKSLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            8628..9644
                     /gene="galE"
                     /locus_tag="ACHLAG_002180"
     CDS             8628..9644
                     /gene="galE"
                     /locus_tag="ACHLAG_002180"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP0655413.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9654..10700
                     /gene="galT"
                     /locus_tag="ACHLAG_002181"
     CDS             9654..10700
                     /gene="galT"
                     /locus_tag="ACHLAG_002181"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFP0655414.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESHDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREERLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10704..11852
                     /gene="galK"
                     /locus_tag="ACHLAG_002182"
     CDS             10704..11852
                     /gene="galK"
                     /locus_tag="ACHLAG_002182"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFP0655415.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11846..12886
                     /gene="galM"
                     /locus_tag="ACHLAG_002183"
     CDS             11846..12886
                     /gene="galM"
                     /locus_tag="ACHLAG_002183"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFP0655416.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDVLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            13091..13843
                     /gene="gpmA"
                     /locus_tag="ACHLAG_002184"
     CDS             13091..13843
                     /gene="gpmA"
                     /locus_tag="ACHLAG_002184"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP0655417.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(14001..15053)
                     /gene="aroG"
                     /locus_tag="ACHLAG_002185"
     CDS             complement(14001..15053)
                     /gene="aroG"
                     /locus_tag="ACHLAG_002185"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFP0655418.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            15369..15749
                     /gene="ybgS"
                     /locus_tag="ACHLAG_002186"
     CDS             15369..15749
                     /gene="ybgS"
                     /locus_tag="ACHLAG_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFP0655419.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15863..16804
                     /gene="zitB"
                     /locus_tag="ACHLAG_002187"
     CDS             15863..16804
                     /gene="zitB"
                     /locus_tag="ACHLAG_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFP0655420.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16801..17520)
                     /gene="pnuC"
                     /locus_tag="ACHLAG_002188"
     CDS             complement(16801..17520)
                     /gene="pnuC"
                     /locus_tag="ACHLAG_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFP0655421.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17558..18601)
                     /gene="nadA"
                     /locus_tag="ACHLAG_002189"
     CDS             complement(17558..18601)
                     /gene="nadA"
                     /locus_tag="ACHLAG_002189"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFP0655422.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18850..18975)
                     /locus_tag="ACHLAG_002190"
     ncRNA           complement(18850..18975)
                     /ncRNA_class="other"
                     /locus_tag="ACHLAG_002190"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(19006..19081)
                     /locus_tag="ACHLAG_002191"
     tRNA            complement(19006..19081)
                     /locus_tag="ACHLAG_002191"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19046..19048),aa:Lys,seq:ttt)
     gene            complement(19115..19190)
                     /locus_tag="ACHLAG_002192"
     tRNA            complement(19115..19190)
                     /locus_tag="ACHLAG_002192"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19155..19157),aa:Lys,seq:ttt)
     gene            complement(19337..19412)
                     /locus_tag="ACHLAG_002193"
     tRNA            complement(19337..19412)
                     /locus_tag="ACHLAG_002193"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19377..19379),aa:Lys,seq:ttt)
     gene            complement(19416..19491)
                     /locus_tag="ACHLAG_002194"
     tRNA            complement(19416..19491)
                     /locus_tag="ACHLAG_002194"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19456..19458),aa:Val,seq:tac)
     gene            complement(19641..19716)
                     /locus_tag="ACHLAG_002195"
     tRNA            complement(19641..19716)
                     /locus_tag="ACHLAG_002195"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19681..19683),aa:Lys,seq:ttt)
     gene            complement(19719..19794)
                     /locus_tag="ACHLAG_002196"
     tRNA            complement(19719..19794)
                     /locus_tag="ACHLAG_002196"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19759..19761),aa:Val,seq:tac)
     gene            complement(19930..20005)
                     /locus_tag="ACHLAG_002197"
     tRNA            complement(19930..20005)
                     /locus_tag="ACHLAG_002197"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19970..19972),aa:Lys,seq:ttt)
     gene            complement(20170..20961)
                     /gene="cpoB"
                     /locus_tag="ACHLAG_002198"
     CDS             complement(20170..20961)
                     /gene="cpoB"
                     /locus_tag="ACHLAG_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFP0655423.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(20971..21492)
                     /gene="pal"
                     /locus_tag="ACHLAG_002199"
     CDS             complement(20971..21492)
                     /gene="pal"
                     /locus_tag="ACHLAG_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFP0655424.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMY
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(21527..22819)
                     /gene="tolB"
                     /locus_tag="ACHLAG_002200"
     CDS             complement(21527..22819)
                     /gene="tolB"
                     /locus_tag="ACHLAG_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFP0655425.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(22952..24217)
                     /gene="tolA"
                     /locus_tag="ACHLAG_002201"
     CDS             complement(22952..24217)
                     /gene="tolA"
                     /locus_tag="ACHLAG_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFP0655426.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            complement(24282..24710)
                     /gene="tolR"
                     /locus_tag="ACHLAG_002202"
     CDS             complement(24282..24710)
                     /gene="tolR"
                     /locus_tag="ACHLAG_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFP0655427.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(24714..25406)
                     /gene="tolQ"
                     /locus_tag="ACHLAG_002203"
     CDS             complement(24714..25406)
                     /gene="tolQ"
                     /locus_tag="ACHLAG_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFP0655428.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGVSRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(25403..25807)
                     /gene="ybgC"
                     /locus_tag="ACHLAG_002204"
     CDS             complement(25403..25807)
                     /gene="ybgC"
                     /locus_tag="ACHLAG_002204"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP0655429.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(25957..26250)
                     /gene="ybgE"
                     /locus_tag="ACHLAG_002205"
     CDS             complement(25957..26250)
                     /gene="ybgE"
                     /locus_tag="ACHLAG_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFP0655430.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(26250..26363)
                     /gene="cydX"
                     /locus_tag="ACHLAG_002206"
     CDS             complement(26250..26363)
                     /gene="cydX"
                     /locus_tag="ACHLAG_002206"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFP0655431.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(26378..27517)
                     /gene="cydB"
                     /locus_tag="ACHLAG_002207"
     CDS             complement(26378..27517)
                     /gene="cydB"
                     /locus_tag="ACHLAG_002207"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP0655432.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(27533..29101)
                     /gene="cydA"
                     /locus_tag="ACHLAG_002208"
     CDS             complement(27533..29101)
                     /gene="cydA"
                     /locus_tag="ACHLAG_002208"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFP0655433.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT         7313 a         7787 c         7242 g         7079 t
ORIGIN      
        1 gctcaacgat taataacatt ttttcattaa tttaagttgg ctaaatgcag ttaaaatttt
       61 taacgaccag ccacccaaaa tcgtcgcttc cattttccgg agtaataatg aagcatgaat
      121 tatcaagtat gaaggcattt gtcatactgg cagagtccag ttcatttaac aatgccgcta
      181 aattactcaa tattacgcaa cctgcattaa cgcgaagaat aaaaaagatg gaggaagatt
      241 tacatattca actatttgag cgtacaactc gcaaggttac gttaacaaaa gcaggaaaaa
      301 ggttgctccc ggaggcgcgg gaattaataa agaaattcga tgaaacgctt tttaatattc
      361 gtgatatgaa tgcttatcat cgtggtatgg tgacgttagc gtgtattccg accgcagtat
      421 tttatttttt accgctggca atcggtaaat ttaatgagct gtatcccaat attaaagtgc
      481 ggattctgga acaaggcacg aataattgca tggaatcggt gctgtgtaac gaatcagact
      541 ttggcataaa catgaacaac gtcacaaatt catccattga ttttacccca ctggtcaacg
      601 agccattcgt gctggcctgt cggcgtgacc atccattggc caaaaagcag cttgtagaat
      661 ggcaggagtt agtgggttac aaaatgattg gcgtacgttc ctccagcggc aaccgattgt
      721 tgatagaaca acagctagcc gacaagccct ggaagctgga ctggttttac gaagtgcgtc
      781 atctttcgac gtcattagga ctagtggagg caggactggg gatttcggcg cttcctggtc
      841 tggcaatgcc ccatgcgccc tattcttcga ttattggtat cccactggtg gaacccgtta
      901 tacgtcggac attggggatt attcgccgca aagatgccgt gctttctccg gcagctgaac
      961 gcttttttgc cttgctgatt aatttgtgga ctgacgataa agacaatttg tggaccaata
     1021 tcgtcgaacg ccagagacat gcgttacagg agataggtta atacaccggc gcaggagaga
     1081 ctgcgccggt taaatcagcg gttagtgtgc gttaaccacc acccacattg gtccctgccc
     1141 gaccgcataa cggccttttt catgcagtag tccctgctcg ccaacaattt cgtataccga
     1201 gatgtggtga gatttttgcc cggcggcaat cagatacttg ccgctgtgat caacattgaa
     1261 gccgcgcggc tgggtttccg ttggctggaa gccttcttta ctcaacacgc tgccatcttc
     1321 cgaaacgctg aaaacggtaa tcaggctggc ggtacggtcg caggcgtata aatggcgacc
     1381 atccggggtg atatgaatat cagccgccca acgggtgtcg gagaagtttt ccggcatcat
     1441 atccagcgtc tggacacatt cgatattacc gtgcggatct ttcagttccc agacatccac
     1501 tgagctgttt aactcattga cgcaatacgc atattgttcg tttggatgga ataccatatg
     1561 acgcgggccg gccccttcaa cggtggtcac ttccgcaggg tcctgcgcca cgagatgacc
     1621 atcatcgctg accgtaaaca ggcaaatgcg atcctgcttt aatgccggaa cccacagcgt
     1681 acggttgtcc ggtgagatat tggcggaatg gcaaccgtcc agcccctcga ccacatcgac
     1741 gacgcccact ggcaggccat cttccagacg cgttacgctc acgttacccg cattgtaaga
     1801 acctacaaag acaaactgcc cctggtgatc ggtggaaata tgcgtcggac tacccggcag
     1861 cgcagactct gcggcaaagg tcagtgcgcc atcgtccggg gcgatacgat acgccaggac
     1921 gcgaaactca gggcgaacac caacatagag ataacgtttg tccgggctga ccaccatcgg
     1981 ctgcacctgc cccggcacat cgacaacctg tgtcagcgtc agtgcgcctt catgattcag
     2041 gttccagacg tgaatttgct ggctctcagg gctggcgata taaactgttt gcttcatgaa
     2101 tgctcctttg catttagcaa ctgactgtaa cagctaaaat tagtcgcttt tggcggtgaa
     2161 tgctgaatta aaacgcagaa ttttgtccga tagtggtatc ggtgtaccat tcgcgcagac
     2221 gaaaattctt aaccttaatc tggaatacgc catgaccaca cgcgtgattg ctctcgactt
     2281 agacggcacc ttattgaccc cgaaaaagac cctgcttcct tcatcgatag aagccctggc
     2341 ccgcgctcgc gaagcaggct atcaattaat catcgtcaca ggtcgccatc acgtcgctat
     2401 tcatcctttt tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta
     2461 tttgtatgat tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc
     2521 cctgcaactc attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtggatga
     2581 tgcaatggtc tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac
     2641 cctgccgccg gaacagcgtc cgactttcac acaagtcgct tctctggctg aaacggcgca
     2701 acaagttaac gccgtatgga agttcgccct gacgcacgat gacctgccgc aattgcagca
     2761 ttttggtaag catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca
     2821 ggttgatatt gcacgcggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc
     2881 gcaaggctgg tcgatggaaa acgtcgtggc gttcggcgat aactttaatg atatcagtat
     2941 gctggaggcc gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg
     3001 cgccaacatt gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca
     3061 cctgatttaa tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag
     3121 gtttgatcac cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttacctc
     3181 cgacttccag ttccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc
     3241 gcaatacgtt acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag
     3301 cctgaatgcg gatacgtagc gcagcttgca gcggttcgtc cagcttattg acccacaaat
     3361 gctgatcgcc cagcgccagc gcagtcatcg cgtaatgcgg atgatgctca agtaccgtca
     3421 ctttcagaat gctactttgt tgctctttcg gcagccacgg attcatcacg ctactacccc
     3481 agacttcttc cagtgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt
     3541 ctgccagatg gaggatctca tccagcgaat ggctgacata caacatcggg atgttgattt
     3601 cccgcgtcag acgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg
     3661 ccagcggttc atccagcaac agcaattctg gtgccgtcag caaagcccgc ccaatcgcta
     3721 cgcgctgttt ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc
     3781 ctaaaagcgc caccagctta tcgaactgat cgaccatgct tttcgccatg ccgtagcgca
     3841 gattgccacg cactttgtaa tgcggaaaca gccgcgcatc ctgaaaaaca tagccaacgc
     3901 gacgcttttc tggcgtcagg cagatacctt tttcggcatc atttagtacc cgaccattga
     3961 ggacaatcct ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt
     4021 ttccggcacc ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa
     4081 tggtcaggca atggttgccc aaggtctggg aaaaattcag ttccagcatg attagcgccc
     4141 cgcccgttca cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag
     4201 cgcaatagaa ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag
     4261 ggtatacatg gcagaaggaa tggttcgcgt ttcaccagga atgttcgaca caaaggtgat
     4321 ggttgcgcca aattcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc
     4381 cggtaaggtc agcggtatcg tgatagtaaa gaaaacgcgc cagcgcccgg cccccagtgt
     4441 tcttgcggcc tgttccagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac
     4501 catcagcgga aacgacatga cggcggcagc gagaaccgcg ccgcgccagc taaaggcgaa
     4561 ggtaatgcca aaccagtcat acagacgttc accgataaac ccgcgccgtc ccatcgaaac
     4621 taataataag taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc
     4681 gagcagagct ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa
     4741 cggcaggcta aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca
     4801 ttctggatcg gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgca
     4861 gcctgcggcc ctttcaaata atcataaaat gctttcactg tcgcattgtt atgcccttcc
     4921 acaacagcaa ccgggtattc cactttttta tgcgaatctt ccgggaaggt ggcgaccact
     4981 tttacccctt tgctggcaac tgcgtcagaa ccgtaaacta tgcccagcgg cgcttcattg
     5041 cgttcgacca acgccagcgc gccacgaaca tcttccgccg gagccagttt cggagagagc
     5101 gtatcccatg cgcccagttt ttgcagtgct tcttttgcat aaatgccagc gggaacatgt
     5161 tccggatcgc caaccgccag acgaccgcca ctcagcagcg aagtccagtt ggttttgctg
     5221 tcgatggtga aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg
     5281 agcagtgtct gacgcgtagc agtatcgatc gcttttttat caactgcata atccatccat
     5341 ttctgatcgg cagaaataaa cagatccgca ggcgcacccg cttcaatctg acgggcgaga
     5401 gtagatgacg aagcgaaaga agaaaccaca tccacgcctt tttctttttt atactgcgta
     5461 gcaatgtcct gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca
     5521 tctgccagtg tattgccagc aacagcgaaa gagagtgccg ccccggcaaa caagttcaac
     5581 catttacgag ccattggtaa ccccttaatg taacgttata taggtgacaa tataacggct
     5641 agctcagaat tttcccagca gttattgcta acctttgtga ggtagataag aaaaaatatc
     5701 ggcaggaaaa gcaggaagtt gagaaaaaga aaatgcccga ctaagcgggc attcagggaa
     5761 tcaatgattt tgtccgggct ggtctttttt accaacacca gaaaagatgt tgaatacttc
     5821 accaagaccg taaatcagac ccaggatgat ggccatcacg acaggtacca tgattacggc
     5881 gaataccaga ctttttaata actctaacat ggtcaactcc agatatagtc atgagactat
     5941 tctaaccgct aagcacagaa aagcactccc cttttgtgcg gtcagctttg cgtggcgttc
     6001 gttttccgtc acaataagac ttttgccagg acattgttat gcaggccgaa atccttctca
     6061 cccttaagct ccaacaaaaa ttattcgccg acccgcgccg gatttcgcta ctaaaacaca
     6121 ttgcgctttc cggttccatt agccagggag cgaaagatgc cggtatcagc tataaaagcg
     6181 cctgggatgc cattaacgag atgaatcagt taagtgagca tattctggtc gagcgcgcaa
     6241 caggcggtaa aggtggcggc ggcgcagtac tgacccgcta tggtcagcga ctgattcagc
     6301 tctatgactt actggcgcaa atccagcaaa aagcctttga tgtgttaagt gacgatgacg
     6361 ccctgccgct gaacagcctg ctggccgcga tctcacgttt ttcactgcaa accagcgccc
     6421 gtaaccagtg gttcggcacc atcaccgccc gcgatcatga tgacgttcaa cagcatgttg
     6481 atgtcttact ggctgacgga aaaacacgcc tgaaagtcgc aattaccgca caaagcggcg
     6541 cgcgtctggg gctggatgaa ggcaaagaag tgttgatatt gctaaaagcg ccgtgggtag
     6601 gtattactca ggacgaggcg gtcgcgcaaa acgctgacaa ccaattaccg ggtattatta
     6661 gtcatattga gcgcggcgca gagcagtgcg aagtattaat ggcgctaccc gacgggcaaa
     6721 cactgtgcgc cacagtgccg gtaaatgaag cgacttctct tcagcaaggg cagaatgtca
     6781 cggcctactt taatgccgac agcgtgatta tcgccacgct gtgctaagcg tgttgacaat
     6841 ttgttatgaa acacgtatcc ctgtcagtaa tcgctgcaca aaatgggata taaaatgtca
     6901 tcgttgcaaa ttttgcaagg cacgtttcgt cttagcgaca caaaaacgct gcaattgcct
     6961 cagctaacgt taaacgcggg tgatagttgg gcgtttgtcg gttcgaatgg aagcgggaaa
     7021 tcggccctgg cccgcgcgct ggcgggggaa cttccgcttt tgaaaggtga acggcaaagc
     7081 cagttttccc acatcactcg tctctccttc gagcaattgc aaaaactcgt cagcgacgaa
     7141 tggcagcgga ataacaccga tatgctcggc cctggcgaag atgacaccgg acgcactacg
     7201 gctgagatca ttcaggatga agtaaaggat gcaccgcgtt gcatgcaact ggcgcagcag
     7261 ttcggtatta ccgccctcct cgaccgacgc tttaaatacc tttccactgg cgagacgcga
     7321 aaaagcctgc tgtgtcaggc gctgatgtcg gagcctgact tgttgattct tgatgagccg
     7381 ttcgatggcc tggatgttgc ctcacgtcag cagctggctg agcgactcgc ctcgttacat
     7441 cagtccggta ttactctggt actggtgctc aatcgcttcg atgagatccc ggagtttgtc
     7501 cagtttgctg gcgtgctggc ggattgcacg ttagcggaaa ctggcgctaa agaggaactg
     7561 ctccaacaag cactcgtcgc gcaactggcg catagtgaac agcttgaagg tgtgcaactg
     7621 ccggagccgg atgaaccttc agcacgtcac gccttacccg ccaacgaacc gcgcattgtg
     7681 ctgaacaatg gcgtggtttc ttataacgat cgccccattc ttaataacct tagctggcag
     7741 gtgaatccag gcgaacactg gcaaattgtc gggccaaatg gtgcaggaaa atcgacgtta
     7801 ttaagcctgg ttactggcga tcatccgcaa ggttacagca acgatttgac gcttttcgga
     7861 cgacgtcgcg gcagcggcga aaccatctgg gatatcaaaa agcatatcgg ttacgtcagc
     7921 agtagtttgc atctggatta ccgggtcagt actactgtgc gtaatgtgat tctttctggc
     7981 tattttgatt cgattggcat ttatcaggcc gtttcggatc gccagcaaaa actggtgcag
     8041 cagtggctgg atattctcgg cattgataaa cgcacggctg acgctccttt ccatagtctt
     8101 tcctggggac agcagcgtct ggcgctgatc gtccgcgcac tggtgaaaca tccgacgttg
     8161 cttattctcg atgaaccact acaggggctt gatccgctca atcgccagct tatccgccgt
     8221 tttgttgatg tgctgattag cgaaggtgaa acgcaattgt tgtttgtttc gcaccacgct
     8281 gaagatgcgc ctgcctgtat tacccatcgc cttgagttcg tgccggacgg tggactctat
     8341 cgctatgtgc tgacaaaaat atattgagtc ggtagtgctg accttgccgg aggcggccta
     8401 agcgccctct ccggccaacg gttcgacgca tgcaggcatg aaaccgcttc ttttttcaga
     8461 taaaaagcgc aatcattcat aaaccctctg ttttataatc acttaatcgc gcataaaaaa
     8521 cggctaaatt cttgtgtaaa cgattccact aatttattcc atgtcacact tttcgcatct
     8581 ttgttatgct atggttattt cataccataa gcctaatgga gcgaattatg agagttctgg
     8641 ttaccggtgg tagcggttac attggaagtc atacctgcgt gcaattactg caaaacggtc
     8701 atgatgtcat cattcttgat aacctctgta acagtaagcg cagcgtactg cctgttatcg
     8761 agcgtttagg cggcaaacat ccaacgtttg ttgaaggcga tattcgtaac gaagcgttga
     8821 tgaccgagat actgcacgat cacgctatcg acaccgtgat ccacttcgcc gggctgaaag
     8881 ccgtcggcga atcggtacaa aaaccgctgg aatattacga caacaatgtc aacggcactc
     8941 tgcgcctgat tagcgccatg cgcgccgcta acgtcaaaaa ctttattttt agctcctccg
     9001 ctaccgttta tggcgatcag cccaaaattc catacgttga aagcttcccg accggcacac
     9061 cgcaaagccc ttatggcaaa agcaaattga tggtggaaca gatcctcacc gacctgcaaa
     9121 aagcccagcc ggactggagc attgccctgc tgcgctactt caacccggtt ggcgcgcatc
     9181 cgtcgggcga tatgggcgaa gatccgcaag gcattccgaa taacctgatg ccatacatcg
     9241 cccaggttgc tgtaggccgt cgcgactcgc tggcgatttt tggtaacgat tatccgaccg
     9301 aagatggtac tggcgtacgc gattacatcc acgtaatgga tctggcggac ggtcacgtcg
     9361 tggcgatgga aaaactggcg aacaagccag gcgtacacat ctacaacctc ggtgctggcg
     9421 taggcagcag cgtgctggac gtggttaatg ccttcagcaa agcctgcggc aaaccggtta
     9481 actatcattt tgcaccgcgt cgcgaaggcg accttccggc ctactgggcg gacgccagca
     9541 aagccgaccg tgaactgaac tggcgcgtaa cgcgcacact cgatgaaatg gcgcaggaca
     9601 cctggcactg gcagtcacgc catccacagg gatatcccga ttaaggaacg accatgacgc
     9661 aatttaatcc cgttgatcat ccacatcgcc gctacaaccc gctcaccggg caatggattc
     9721 tggtttcacc gcaccgcgct aagcgcccct ggcagggggc gcaggaaacg ccagccaaac
     9781 aggtgttacc tgcgcacgat ccagattgct tcctctgcgc aggtaatgtg cgggtgacag
     9841 gcgataaaaa ccccgattac accgggactt acgttttcac taatgacttt gcggctttga
     9901 tgtctgacac gccagatgcg ccagaaagtc acgatccttt gatgcgttgc cagagcgcgc
     9961 gcggcaccag ccgggtgatc tgcttttcac cggatcacag taaaacgctg ccagagctca
    10021 gcgttgcagc attgacggaa atcgtcaaaa cctggcagga gcaaaccgca gaactgggga
    10081 aaacgtaccc atgggtgcag gtttttgaaa acaaaggcgc tgcgatgggc tgctctaacc
    10141 cgcatccgca cggtcagatt tgggcaaata gcttcctgcc taacgaagct gagcgcgaag
    10201 aacgcctgca aaaagaatat tttgccgaac agaaatcacc aatgctggtg gattatgttc
    10261 agcgcgagct ggcagacggt agccgtaccg ttgtcgaaac cgaacactgg ttagccgtcg
    10321 tgccttactg ggctgcctgg ccgttcgaaa cgctactgct gcccaaagcc cacgttttgc
    10381 ggatcaccga tttgaccgac gcccagcgca gcgatttggc gctggcgttg aaaaagctga
    10441 ccagtcgtta tgacaacctc ttccagtgct ccttccccta ctctatgggc tggcacggcg
    10501 caccatttaa tggcgaagag aatcaacact ggcagctgca cgcgcacttt tatccgcctc
    10561 tgctgcgctc cgccaccgta cgtaaattta tggttggtta tgaaatgctg gcagagaccc
    10621 agcgagacct gaccgcagaa caggcagcag agcgtttgcg cgcagtcagc gatatccatt
    10681 ttcgcgaatc cggagtgtaa gaaatgagtc tgaaagaaaa aacacaatct ctgtttgcca
    10741 acgcatttgg ctaccctgcc actcacacca ttcaggcgcc tggccgcgtg aatttgattg
    10801 gtgaacacac cgactacaac gacggtttcg ttctgccctg cgcgattgat tatcaaaccg
    10861 tgatcagctg tgcgccacgc gatgaccgta aagttcgcgt aatggcagcc gattatgaaa
    10921 atcagctcga cgagttttcc ctcgatgcgc ccattgtcgc gcatgaaaac tatcaatggg
    10981 cgaactacgt tcgtggcgtg gtgaaacatc tgcaactgcg taacaacagc ttcggcggcg
    11041 tggacatggt gatcagcggc aatgtgccgc agggtgccgg gttaagttct tccgcttcac
    11101 tggaagtcgc ggtcggaacc gtattgcagc agctttatca tctgccgctg gacggcgcac
    11161 aaatcgcgct taacggtcag gaagcagaaa accagtttgt tggctgtaac tgcgggatca
    11221 tggatcagct aatttccgca ctcggcaaga aagatcatgc cttgctgatt gactgccgct
    11281 cactggggac taaagcagtt tccatgccga aaggtgtggc tgtcgtcatc atcaacagta
    11341 acttcaaacg taccctggtt ggcagcgaat acaacacccg tcgtgaacag tgcgaaaccg
    11401 gtgcgcgttt cttccagcag ccagctctgc gcgatgtcac cattgaagag ttcaacgctg
    11461 ttgcacatga gctggaccca atcgtggcga aacgcgtgcg gcatatcctg actgaaaacg
    11521 cccgcaccgt tgaagctgcc agcgcgctgg agcaaggcga cctgaaacgt atgggcgagt
    11581 tgatggcgga atctcatgcc tctatgcgcg atgatttcga aatcaccgtg ccgcaaattg
    11641 acactctggt agaaatcgtc aaagctgtga ttggcgacaa aggtggcgta cgcatgaccg
    11701 gcggcggatt tggcggctgt atcgtcgcgc tgatcccgga agagctggtg cctgccgtac
    11761 agcaagctgt cgctgaacaa tatgaagcaa aaacaggtat taaagagact ttttacgttt
    11821 gtaaaccatc acaaggagca ggacagtgct gaacgaaact cccgcactgg cacccgatgg
    11881 tcagccgtac cgactgttaa ctttgcgtaa caacgcaggg atggtagtca cgctgatgga
    11941 ctggggtgcg actttacttt cagctcgtat tccgctttcc gatggcagcg tccgcgaggc
    12001 gctgctcggc tgtgccagcc cggaatgcta tcaggatcag gctgcgtttc tgggggcctc
    12061 tattggtcgt tatgccaacc gcatcgccaa tagccgttat acctttgacg gtgaaaccgt
    12121 gacgctttcg ccaagtcagg gcgttaacca gctgcacggc ggcccggaag ggttcgacaa
    12181 acgtcgctgg cagattgtga accagaacga tcgtcaggtg ctgtttgccc tgagttcaga
    12241 tgatggcgat cagggcttcc cgggtaatct cggcgcgacg gtgcaatatc gtctgaccga
    12301 cgataaccgt atctctatta cttatcgcgc cacagttgat aaaccttgcc cggtgaatat
    12361 gactaatcac gtctatttca atcttgacgg cgagcagtct gacgtgcgca atcacaagtt
    12421 gcagattctg gcggacgaat atctgccggt tgatgaaggc ggcattccgc acgacgtcct
    12481 gaaatctgtc gccggaacgt cttttgattt ccgtagcgcc aaaatcatcg ccagtgagtt
    12541 tcttgccgac gacgatcagc gcaaagtgaa aggttacgat cacgcattct tgttacaggc
    12601 caaaggcgat ggcaagaaag tggcggcgca tgtctggtca gcagatgaaa aattgcagct
    12661 gaaggtctac accaccgctc cggctctgca attctactcc ggcaacttcc tcggcggcac
    12721 gccatcaaga ggaactgaac cttacgccga ctggcaaggc ctggcactgg aaagcgagtt
    12781 tctgccggac agcccaaacc accctgaatg gccgcaaccg gactgcttcc tgcgtcctgg
    12841 cgaagagtat tccagcctga cggaatatca gtttattgct gagtagcacc tgaagccctc
    12901 gtaatgaggg ctttatcctt atattgctca aatcaccagc aaacaccgac atatttgcaa
    12961 ctcaatattc acaacaacct tacactgcga cactattttc gctatggtta tgcgtaagca
    13021 ttgctgttgc ttcgtcacgg caatataatg agaattatta tcattaaaaa atgatttgag
    13081 gagtaagtat atggctgtaa ctaagctggt tctggttcgt catggcgaaa gtcagtggaa
    13141 caaagaaaac cgtttcaccg gttggtacga cgtggatctg tctgagaaag gcgtaagcga
    13201 agcaaaagca gcaggtaagc tgctgaaaga ggaaggttac agctttgact ttgcttacac
    13261 ctctgtgctg aaacgcgcta tccataccct gtggaatgtg ctggacgaac tggatcaggc
    13321 atggctgccc gttgagaaat cctggaaact gaacgaacgt cactacggtg cgttgcaggg
    13381 tctgaacaaa gcggaaactg ctgaaaagta tggcgacgag caggtgaaac agtggcgtcg
    13441 tggttttgca gtgactccgc cggaactgac taaagatgat gagcgttatc cgggtcacga
    13501 tccgcgttac gcgaaactga gcgaaaaaga actgccgctg acggaaagcc tggcgctgac
    13561 cattgaccgc gtgatccctt actggaatga aactattctg ccgcgtatga agagcggtga
    13621 gcgcgtgatc atcgctgcac acggtaactc tttacgtgcg ctggtgaaat atcttgataa
    13681 catgagcgaa gaagagattc ttgagcttaa tatcccgact ggcgtgccgc tggtgtatga
    13741 gttcgacgag aatttcaaac cgctgaaacg ctattatctg ggtaatgctg acgagatcgc
    13801 agcgaaagca gcggcggttg caaaccaggg taaagcgaag taaacgtcat tcgtttaaaa
    13861 tgagaaagcc gactgcaagt gagtcggcat ttttgttgct aacaatggag cacattgcct
    13921 gatgcgacgc tgcgcgactt atcaggcctg tggtgattca tcggatacgc cactctgacg
    13981 gcgcatccga caattaaacc ttacccgcga cgcgctttta ctgcattcgc cagttgacgt
    14041 aacagagcat cggtatcttc ccagccgatg caggcatcgg tgatgctctt accgtaggcc
    14101 agcggctccc cgctctcgag gctctgattg ccttccacca gatggctttc caccatcacg
    14161 ccaataatgg ccttttcgcc accggcaatc tgctggcaaa cgtcagcaca aacatccatc
    14221 tgctttttga attgtttgga cgagttagca tggctgaaat cgatcatcac ctgtgctggc
    14281 aggcctgctt tgttcagccc ttctttcact tcagcaacgt gcttcgcgct gtagttaggc
    14341 tctttaccgc cgcgcagaat gatatggcaa tcgccgttac cgctggtatt cacaatcgcc
    14401 gaatgccccc atttcgttac ggacaggaag cagtgcggcg caccggcggc attaatggca
    14461 tcgatagcca ctttaatcgt accgtcggtg ccatttttga agccgaccgg acaagaaagc
    14521 cctgatgcca gttcgcggtg cacctgcgat tcggtggtac gtgcgccaat tgcgccccag
    14581 ctcatcaggt cagcgagata ttgtggggtg atcatatcga gaaactcacc tgccgctggc
    14641 agaccgctgt cgttaatatc aagcagcaat ttacgggcta tacgcagacc gtcgttgatc
    14701 tggaagctat tatccatatg cggatcgtta atcagccctt tccagcccac cgtggtacgc
    14761 ggcttttcaa aatagacgcg cattacgatt tccagctcat ctttcagctc ttcacgcagc
    14821 gccagcaagc gagtggcata ctcttttgcc gcgacaggat catgaattga gcatgggcca
    14881 atcacaacca acaggcgatc atcattacct ttcaggatct tatggatcgc ttttcgggca
    14941 tgggcaaccg tattcgcggc attttcagta gcggggaatt tttccagcaa tgcgacagga
    15001 ggaagtaact ctttgatttc tttgatgcgt aaatcgtcgt tctgataatt catgtctgtt
    15061 ccagtgttgc catacttatc ttagtgaatg caatacttcc aatctatatc ttccgtcaga
    15121 atgtgtaaac ggggttttac actatgaacg gattaatcct ggaattagca agaaaaacgc
    15181 cagattgtcg cgaaaaacga gatctctcct acaatttcta actgtaactc ctttgtttgt
    15241 taattatttc aagattctct gctgcgtttc ataacctggc tgaaatctta aaccaatgcc
    15301 ttatattcac ctgcaaatgc actgttggaa gaggttatcc gacataacga ccataacagg
    15361 agcatcctat gaaaatgaca aaactggcca cattatttct gactgccact ctaagccttg
    15421 ccagcggtgc cgcactggct gccgatagcg gagcgcaaac taataacggc caggcaaacg
    15481 ccgcagctga tgcgggccag gtagcccctg acgcccgtga aaatgtcgcg ccaaataacg
    15541 tcgacaataa cggggtaaat accggttctg gtggcacaat gctgcattcg gatggttctt
    15601 caatgaacaa tgacggaatg accaaagatg aagagcacaa gaacaccatg tgcaaagatg
    15661 gtcgctgtcc ggatatcaat aaaaaggtac aaaccggtga tggcatcaat aatgatgtcg
    15721 ataccaaaac cgacggtacc acgcagtaac cctgcaaaat agccaataat gggagagcgg
    15781 agtctctccc ttttaatttt tcacttctaa aagcagtatg atgtcataaa gttaagtgag
    15841 aataattaag ggatgactcg tcatggcgca ctcacactca cacacgtcct cacacctgcc
    15901 agaagataat aatgctcgtc gcttgttgta tgctttcggc gtgactgccg ggtttatgct
    15961 ggtagaagtc gttggtggtt ttctttctgg ttctctggca ttgctggccg atgcgggtca
    16021 tatgttgacc gatactgccg ccctgctttt cgccctactc gccgtgcaat tttcacgtcg
    16081 tcctcccacc attcgccaca ctttcggttg gctaagactg accacgctcg cggcgtttgt
    16141 gaatgccata gccctcgtgg taattaccat tttgattgtc tgggaggcga tagaacgctt
    16201 ccgcacgccg cgtccggtcg agggcggcat gatgatggca attgccgtgg ctgggttgct
    16261 ggcaaatata ctttctttct ggttacttca tcacggcagc gaagagaaaa acctcaacgt
    16321 acgagcagcg gcactgcatg tgctcgggga cctgctgggt tcggttgggg ctattattgc
    16381 cgcgttgatt attatctgga ctggctggac acctgctgac cccattctct cgatactggt
    16441 gtcgcttctg gttctgcgta gcgcgtggcg actgttgaaa gatagcgtga atgaattact
    16501 tgaaggtgca ccggtatcgc tggatatcgc tgaactgaag cgtcgtatgt gccgggaaat
    16561 cccggaagtc cgcaatgtgc accatgtaca tgtatggatg gtaggcgaga agccggtgat
    16621 gacgttgcat gtgcaggtga tcccgccaca cgatcacgat gccttgttgg atcagatcca
    16681 acactacctg atggatcact atcagattga gcacgccacc attcagatgg aatatcaacc
    16741 ttgtcatggg ccagactgcc acctgaatga aggggtgtct ggccattcac atcatcacca
    16801 ttaatgggac agcgcgcgtg agcctctttc acgtgcgctg ttgatccaca tccggctgcc
    16861 gttgagcgca ataaaggtca ggatgatgta ctccagagac atggcgtaaa cgccctgaag
    16921 tgcaaaaata acgacgctaa tcacgttaat aatcacccac aacagccagt tttccacata
    16981 cttacgcgtc atcagaatca ttgccacgat agataacacc atcatgcatg aatcccagaa
    17041 cgggaaagcg tccggttgca gttcaggcat cacaacctgt aatcctaatg cttgcatgat
    17101 catgactgcc acgcgggtca aaaatgcaaa caccggattg ataaataccg tcatcagacc
    17161 aatcgaaaca acgcaaaccg ccaaccagct gagtgccttc ggcaatggca accagcgaat
    17221 tttcaactcc gcctcgttct gactggtttg tcgcgaccac gcataccaac cgtaaatatt
    17281 cgcggcaaag aaaaacacct gtaatagcag gctggcatac agctgaatct gaaagaaaat
    17341 aatgccaaac aaggtgacgt tgatcaggcc aaagaagtag ttgctgatct tctccagact
    17401 ggcaaggcca atacacagca acccggcgat cgtgcctacc gcttcgatcc atgagagatc
    17461 ataaccgcct gcccctattg gtatatgtac caggatattc tgcacactaa aaaaatccat
    17521 ctttccccca aagcgtaacg ttacgcctta ttattcgtta tccacgtagt gtagccgcaa
    17581 aatccagcat acgattgagc ggcaccagcg ccctctctcg cagcctttca tcaacatgaa
    17641 cctcgtgatt gcttccttcc tgttctaatg cctctgcgat ggactgaagg ccattcatgg
    17701 ccatccacgg acaatgcgcg cagctgcggc aggttgcacc ctcacctgcg gttggtgctt
    17761 ccagtaactc tttatctggc actgcctgct gcattttgta gaaaatgccc cgatcggttg
    17821 ccacgataag ccgctgatgt ggcaatgttt tcgcagcagc gatcagttga ctggtggaac
    17881 cgaccgcatc cgccatatcg acaatagctt gtggtgattc tggatgcacc agtatggcag
    17941 catccgggta ttcttcttgc aagcgggtta acgcctgagt cttaaattca tcatgcacaa
    18001 tacaggcacc ctgccagcat agaatgtctc cacccgtctg tttttgcacg taacgcccca
    18061 gatgtttgtc gggtgcccag atgatttttt cacccaaact atcaagatga tcaataagtt
    18121 cgacggcaat gcttgaagtt accacccaat ctgcgcgcgc ttttaccgca gcagaagtgt
    18181 tggcgtagac gacgacagta cgatcgggat gggcatcgca aaatgcgtta aattcttcaa
    18241 cagggcagcc gagatccagt gaacattcag cctgaagtgt cggcatcaga attgtttttt
    18301 ccggactgag aattttggcg gtttctccca taaatctcac cccagcgact aacaaagtag
    18361 aagcgggatg ctttgcaccg aagcgcgcca tttccagaga atcagaaata cagccaccgg
    18421 tttcttctgc cagttgttga atttcgggat cggtatagta gtgggcaacc atcaccgcat
    18481 tacgttcttt tagcagacgt tttatcttct cgcggtaata cgctttttca tcaatgctta
    18541 acggcgtcgg cttcgggggg aaaggataaa tcgccgtgtc tggatcaaac attacgctca
    18601 tcttaccatc tcgttttaca ggcttaacgt taaaaccgac attagcgtaa tattcgctgt
    18661 tatcggtgta atttgtttta tatgctaaac aagatagtcg aatggtgggg aaaagtcact
    18721 cgaattacgc ctaattccac cgacgtagag ttgcttgcac agtaagctct gacgatgtca
    18781 tcaggtttac aaagtgaaat tgaggatgcc tgaatagcaa aaaagcgcct ttagggcgct
    18841 tttttacatt ggtgggtcgt gcaggatgac tcgcttcgct cgcccttcgg gccgtcgccg
    18901 cgagcggctc cgttgtttcg ctgcgctcaa ctcaaacctg ctgcaggttc gaatctttga
    18961 gtatcgcaga tagcaaaaaa gcgcctttag ggcgcttttt tacattggtg ggtcgtgcag
    19021 gattcgaacc tgcgaccaat tgattaaaag tcaactgctc taccaactga gctaacgacc
    19081 ccttgcggga ttatgctgct ttaatcattc aggatggtgg gtcgtgcagg attcgaacct
    19141 gcgaccaatt gattaaaagt caactgctct accaactgag ctaacgaccc atacgggtgt
    19201 tacctgaaag atttctactc ggggccaatc tttaagattg gtgtgtcgtg caggatgact
    19261 cgcttcgctt gccctacggg ccgttgctgg cgcaacgtta tccttcacac ttaacatctg
    19321 agtttgatgt taaaattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    19381 gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc
    19441 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg
    19501 ctggatacta caaactactt ccaccgaacc caccgtaatt agtggtgggt cgtgcaggat
    19561 gactcgcttc gctcgcccta cgggccgttg ctgacgcaac gttatcctac acgcttaaca
    19621 tctgtcttcg atgctaaaac tggtgggtcg tgcaggattc gaacctgcga ccaattgatt
    19681 aaaagtcaac tgctctacca actgagctaa cgacccggtg gtgggtgatg acgggatcga
    19741 accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc acccaatact
    19801 acgctggata ctacaactaa ctatgttagt ggtgggtcgt gcaggatgac tcgacttcgt
    19861 ctcgcccttc gggccgttgc tgatgcaacg ttatccttca cgttttacta ccgcttccac
    19921 cttagcgatt ggtgggtcgt gcaggattcg aacctgcgac caattgatta aaagtcaact
    19981 gctctaccaa ctgagctaac gacccacttt ttcgttgctt tcggtttgtt tgatatcccg
    20041 tggcaacggc ggcatatatt actgatttca gacttgagcg caacaaaaat ttcgatgcag
    20101 atcactcaac tgcttatgat tcgcacgaca cgaccagaaa taatgcgact tctggtcgtg
    20161 tgttatgcat tacatcgcgt tcagacgttt ttgtgcctgt ttagcgccat cagtaccagg
    20221 gtatttactg ataacctgct ggtatacggc tttcgctttt gcggtgtcac ctttgtcctg
    20281 catgatgacg ccgactttaa acatcgcatc agcagccttt ggtgacttcg gatagttttt
    20341 cactaccgaa gcaaaatagt acgccgcatc atctttttta cccttgttgt agtttaactg
    20401 acccagccaa taattggcgt ttggcaggta agttgaatca gggtaatttt tgatgaaatt
    20461 ctgaaatgcc accattgcgt catcctggcg ggatttatcc tgcaccagcg caatagctgc
    20521 attgtaatcc gtgtttgcat cgccgctttt caccggcgcg ccagcattcg cagtaccagc
    20581 atcagctgtc ggcgtcgttg atgccgccgc accgctttga tcgctgctgg ttgattgcgc
    20641 cgctgcacca ccgctgctga ggctgtcgat ctgcaacagg atctgctttt gccgctccac
    20701 gacctgattc agttgatact gattttcctg aatctgacca cgcagggaat caatatcgga
    20761 ttgattatca gaaagttgtt gctggagttg ggttaaaagc tggctgtgag cattagaaat
    20821 acgctcaagt tgagtgacgc ggtcttcgac cgagcctgag ccgacactac tgattggtgc
    20881 ctgagcaaaa gcggcccagg gggccgctat accaaccagt aacgacagac tcaatagttg
    20941 atgtctgaag ttactgctca tgcaattctc ttagtaaacc agtaccgcac gacggttttt
    21001 ggagtatgcc gcttcgtcat gacccagtac tgcaggtttt tctttaccgt aagaaacgat
    21061 ggagatctgg tctgcagaaa cgcctttacc ctgcaggtac atcttaacgg cgttcgcacg
    21121 acgttcaccc agggagatgt tgtattccgg agtaccacgt tcgtccgcgt gaccttctac
    21181 ggtgactttg taagacgggt tgctacgcag gaagtttgca tgtgcatcca gcatttgagc
    21241 gaagtcagaa cggatatcgt acttgtccag atcgaagtaa acgatgttgt tctgctgcag
    21301 ctgttgcatt tgcagacgag cctgctcttc ggaagacatg ttgccgttgc cgccgttcgc
    21361 atacatacca gtgccggcac ccagcatgcc ttcgctgccg tcattgctgg cgttcttgtt
    21421 ggaagaacat gccgcaattg ccataacagg cagagcaatc atcagccctt tcagcacttt
    21481 gttcagttgc atttcaatga ttcctttact attcaattaa ttattatcac agatacggcg
    21541 accaggcagg gaatttgacc tgtccatcag ttgccggaag acgcgctttg aaacgcccat
    21601 ctgtagaaac caaattcagc acggatccca tcccctgaga agagctgtag attaccatag
    21661 tgccgttagg tgccagactt ggcgtttcat ccaggaacgt ggacgacaga acttgtacgc
    21721 ctcccgttgc cagatcttgt ttggcaatgt gctgctgccc accattggag ctgaccatta
    21781 ccataaattt accgtcgctg ctgacatccg catcctggtt ctgcgaacct tcccaggtaa
    21841 tacgttgtgg cgcaccgccg ttgatattca ctttataaac ctgcggacga ccggcctggt
    21901 cagaagtaaa tgccaggttc tggctatccg ggaaccaggt cggttcggta ttgttactgc
    21961 gaccatcagt cacctggcgg atctgaccag aagccaaatc cattacgtac aggttcagac
    22021 taccggtttt cgacaaggcg aatgccagtt tgctgccgtc tggcgagaat gcaggtgcac
    22081 cgttgtgacg cgggaatgaa gccacctgac gtacagcgcc atttgccagc gtctgaataa
    22141 ccagcgcgga acgaccgctt tcgaaggtca cataagccag tttagaaccg tctggtgacc
    22201 acgccggaga catcagcggc tgcggtgaac ggtgaacgac aaactggttg taaccgtcat
    22261 agtcagatac gcgcagttca tacgggaact gaccgccgtt ggtctgaaca acgtaggcaa
    22321 tacgagtacg gaacgcacct ttaatgccgg tcagtttttc aaacacttca tcactggcgg
    22381 tatgaccagc ataacgcagc cactgcttgt tcactttgta cgagttctga gcaagtacag
    22441 tacccggtgc gccgccagtg tcaacaagtt gataagcaac attgtaagaa ccatccggat
    22501 tcggagtgac ctgaccgaca actacagcgt caatgcccag tgcggaccat gcagctggtt
    22561 gtacttcctg cgcactaccc ggctgctgtg gcagacgagc gcgatctaac ggattaaatt
    22621 taccgctgtt acgcaagtca gcagcaacga tgccgccaat atcttcaggt gccgcaccag
    22681 gccccgccca ctggaaagga acaacaccaa taggacgacc ggaatctaca ccgctgtcga
    22741 tcacaatgcg gacttcagca tgcagaactg atgcccacag tatgagaaaa ccaaatgcta
    22801 ctcgtaatgc ctgcttcatc atatctccct tatctggacc gatggcccac gataatttag
    22861 cagaatgtta acaaactaaa atacacaaaa ctaccagaac cccgtggcaa ccggtgcctg
    22921 atgttgaccg tccgaacagt caacatcgcg attacggttt gaagtccaat ggcgcgtttt
    22981 tgaacacttc atatactgcc tggcttggtg gtttcgggat cttcgcaagt ttagctgctg
    23041 ccaacgcagc ctgacaaagt gcgggatcgc caccttcagg tttgatatcc agtaacatac
    23101 catcgggtgc cagttttatg cgcagcgtac aggttttgcc tgcataggac gatgcgtcat
    23161 agaacttact ttcgatagca gatttaatct gcccggcata gttattgata tcggcccctg
    23221 atgcgccatt gtttttagta ttaccactcc cggcaggcga agcattgttc cctttcgccc
    23281 ctccccccgt tttcggtgca ttcttaccag agcttagctc accgaaaata tcatctgcct
    23341 ctgcggccgc ttttgctgca gcggcttttt cagctgcagc ttttgccgct gccgcttttt
    23401 tgtctgcggc ggctttttct gccgctgctt tcttatcagc tgctgccttt tcagcagccg
    23461 ctttttctgc tgcttttttg tcggctgcag ctttctctgc tgccgctttc ttatcagctg
    23521 cagccttttc agcagccgct ttcttctcag cttctgcttt ggctttttca gcagcttcag
    23581 ttgccgcttt ctttcttgct tcagctgcag ctgcttctgc cgcttccgct ttcttcttca
    23641 gtgccgcagc ggctgcctcg gcttttttct gcgcttcggc tgcggctttg gcggcttctg
    23701 cttctgcttt tttctttgcg tctgcagccg ctttcttcgc tgcttcttcc gcagctttag
    23761 catctgcttc ggccttcgct ttagcatctg ccgccgcttt cgctgccgcc tcttcagctt
    23821 gcttctgctt taactcggcc tgttttgcgg cttcttcagc ctgctttttc tgctcctgag
    23881 ccgctaaccg ctctttctca agttgcttca ggcgttcctg ttcagccgct tgtttctcac
    23941 gcagttcttc agcagcctgc tgttccttca tcttgcgctg ttcatcagaa cgcttcgcgc
    24001 ttgattcctg gctttgcatg cgtttgtact gctcaactac cgcacctgaa tcaaccatga
    24061 cagcgtcgat ggacgaacca ccgccgcctc cggctgaagc ttctatattc tcatcgaacg
    24121 aactccagat cagcgccgca aataagatga catgcagcac tgctgaaata attatcgccc
    24181 gcttgagctt gtcgttttgt tcggttgcct ttgacactct cggtttccaa aaactgttcg
    24241 cctgttaccc gctctctttc aagcaaggga aacgcagatg tttagatagg ctgcgtcatt
    24301 aaaccaaccg atttcacacc cgcactatgt aacaagttca gtgctttaat tatttcatcg
    24361 taaggcacat cttttgcgcc accgatcaga aagaccgttt tcgggttggc cttgaaacgg
    24421 ctggacactt ccgccaccac ctgctctggt ggtaaacgct ccaggcgatc tttctcaacc
    24481 accacggtgt actgaccaat accagacact tcaacaatca ctggcggatt atcgttactg
    24541 ctcaccgcct gtgattcagt agcgtctggc agatcgacct ccacgctctg ggtgatgatg
    24601 ggcgctgtcg ccataaagat cagcaacagc accagcagta cgtccagcaa cggtacaatg
    24661 ttgatttcgg acttgagatc gcgacgacct cgtccacgcg ctctggccat ggcttacccc
    24721 ttgttgctct cgctaacggt aaacgcctgg cggtgcagaa tcgcggtaaa ctcttccata
    24781 aagttgtcgt aattcagttc cagtttgttt acgcgctggt tgaggcggtt ataagccatt
    24841 accgccggaa ttgcggcgaa cagaccaatt gcagtcgcaa tcaacgcttc tgcgataccg
    24901 ggcgcaacca tttgcagtgt tgcttgtttt accgccccga gggcgataaa ggcgtgcatg
    24961 atcccccaga ccgtaccaaa cagaccaata tacgggctga tggagccaac cgtaccgagg
    25021 aacggaatgt gcgtttccag attttcaagt tcacggttca tggagatacg catagcacgc
    25081 gacacccctt ccactacggc ttccggcgca tggctattgg cacgatgcag gcgcacaaac
    25141 tctttgaacc cgctgtaaaa gatttgttcc gaaccagtca gattatcccg tttcccctgg
    25201 ctctcttgat agaggcgaga gagttcgatt ccagaccaga atttatcttc aaacgcttcg
    25261 gcttcgcgcg ccgctgcgtt aagaatacgg gtccgctgga taataatggc ccaagatgcg
    25321 attgaaaaac caatcaaaat caacatgata agtttaacca gaaggctagc cttcaggaac
    25381 aaatcaagga tattcatgtc agtcactgct taaactccgc gacaatagac ttgggaagcg
    25441 cacgaggctt cattttgagt gggtcaacgc aaacaaccag aacctctgct tcattcagca
    25501 aagtattctc ggcgttgaca atacgttgcg tgaaaaccaa agaggtgcca cgcattgatg
    25561 ttatttcagt ctgtatttcg agcatatcgt cgagccgcgc aggtgcgtaa tattccaccg
    25621 tcattttacg taccacaaag gcaacgcgtt cagccatcag cgcctgctga ctgaagtgat
    25681 gatgacgcag catctctgtg cgtgctcttt cataaaaagc gacgtaactg gcgtggtaca
    25741 ccacaccacc ggcatcggta tcttcatagt agacgcgaac cggccatcga aacagcgttg
    25801 tattcacttt acatcccggt aatgcaacaa aagttagagc ttttaaacgc tgctactata
    25861 cgcgcgcgat tgaggtttgg gaatgagggc aagttaaggg agcgtaaaaa tttatgggcc
    25921 tggagcggcc cataattttt taaagagaat tctgatttaa aagaagaaga aaatcagccc
    25981 gacaatgaga acaatatcgg caagcaatgg gcaaaaaatc ccttgccaaa gaaccttctg
    26041 cggacgaaag cccacgccgt gaatcacacc agcacagacg gcccacatca gcaataaacc
    26101 atgccagatt tccagttcac tggtcttcgc ggcaaaacga gatgggtccc aaaacataca
    26161 tcctgctaac agaagcgcca tcacgaagga aagcgcccgc agggggcgct tgtccattac
    26221 cgcatacaaa gtcgcgataa tcttactcat cagatgtctt cttgaccggc tttgcctgat
    26281 tcgacgtgtt caagcgccag cgcggtgatt accccaaacg aacaggcaag aagcgttccc
    26341 agaatccatg cgaaatacca catttttagc tccttactta gtacagagag tgggtgttac
    26401 gttcaatatc ttctttggtg atacgaccga acattttcca gtaacaccag gcggtgtaga
    26461 gcagaatgat cggtaccaga accaccgcaa cccaggtcat gacgttaagc gtcagctggc
    26521 tggaagttgc atcccacatt gtcagacttg cgttcatcat ggtgctggac ggcatcacaa
    26581 acgggaacat tgcgataccg gctgtcagga tgatgcaggc cagcgtcagg gaggagaaca
    26641 caaacgccca cgcggcttta tccatacgtg cagtcaggat ggtcagcagc ggcagaacca
    26701 cacccagcgc cggaatagcc cacagaattg gcgtgttgtt gaagttaacc agccatgcgc
    26761 cagcttcacg aaccacttct ttattcagtg ggttagaggc tgcgtaatgg tccattgtcg
    26821 atttcacgac ataaccatcg ataccgtaca tcacccatac gccagccagt gcgaaacaga
    26881 ccagtgtcac cagcgcagcc acctgagccg ttgcacgggt acgcaggtgc agttcgccca
    26941 cggtacgcat ttgcagatag gttgcgccct gagtaatgat catccctacg ctcaccacgc
    27001 ctgccagcag gccgaacggg ttaagcaact ggaagaagtt accggtgtag tacagacgca
    27061 gatattcatc aacgttgaac ggtacgccct gcaacaggtt accgaacgct acaccaatta
    27121 ccagcggcgg aacgaagcta ccaatgaaga tgccccagtc ccacatgtta cgccagcggg
    27181 tttcttcaat cttggagcgg tagtcaaaac cgaccggacg gaagaacaaa gacgccagca
    27241 cgaggatcat cgccacatag aagccggaga acgcagcggc atagaccatc ggccaggcag
    27301 caaagagtgc gccgcccgcg gtgatcagcc aaacctggtt accgtcccag tgtggtgcaa
    27361 tggagttaat cataattcga cgctcggtgt cgttacgacc gaggaaacgg gtgagcatgc
    27421 ccacccccat gtcgaaaccg tcagtgactg caaaaccaat cagcagaacg ccaaccagca
    27481 gccaccagat aaaacgcaat acttcataat cgatcatttg acgactcctg tcttagcgtg
    27541 ccggctgagt agtcgtggaa gactgctcaa agtgatagcg accggttttc aggctgcttg
    27601 ggccgaggcg tgcaaatttg aacattaaga acaattctgc caccaggaac agggtataca
    27661 ggccgcaaat cagcaccatt gagaagatga gatcgcctgc ggtcagtgac gagttcgcca
    27721 cagctgtcgg cagcacttca ccgatagccc acggttggcg gccatattca gccacgaacc
    27781 agcccgcttc tacagcaatc cacggcagcg gaataccgta cagcgcggcg cgcagaagcc
    27841 attttttctc gccaatgcgg ttgcggatga cactccagaa agagagcgcg atgattgcca
    27901 gcagcaggaa gccacacgcc accatgatac ggaacgcaaa gtacagcggc gctacacgcg
    27961 ggatggagtc tttggttgcc tgttgaatct gcgcttcagt cgcatcagcc acgtttggcg
    28021 tatagcgttt cagcagcaga ccgtaaccga ggtctttctt catgctattg aactggtcac
    28081 gaaccgcctg gtcggtagaa ccagaacgca gttgttcgag cagagagtac gccttcatcc
    28141 cgttacgaat gcgttcttca tgctgcacca tcagctcttt caggccgata accggggtat
    28201 ccacggaacg cgttgcaatg atgcccagtg cgtaagggat ctgaatcgca aatttgttcg
    28261 tctcctcttc ctgatcagga atgccgaaca gagtaaaggc agcaggcgca ggttgcgttt
    28321 cccactcggc ttcaatagca gccagtttgg ttttctgcac gtcgcccatt tcgtagccgg
    28381 attcatcacc cagaacaata acagacagaa cagcagccat accgaagctg gcagcgatag
    28441 caaaggagcg tttagcgaag gcgaagtcac gacctttcag catataccat gcgctgatac
    28501 cgaggatgaa catcgcgcca gtcacataac cagacgctac agtgtgaacg aatttcacct
    28561 gagcaaccgg gttaagcacc agctcggaga agctcaccat ctccatacgc atagtttcaa
    28621 agttgaaatc ggacgcgatt gggttttgca tccagccgtt cgcaaccaga atccacagtg
    28681 cggacaggtt tgaaccgagc gccaccagcc aggtgacaca catatgctga actttaccca
    28741 gacgatccca accgaagaag aacagaccta caaaggtgga ttcgaggaag aaggccatca
    28801 gaccttcgat tgccagcggc gcaccgaaga tatcccctac atagtgggaa tagtaagacc
    28861 agttagtccc gaactggaac tccatggtca gaccggtagc cacacccaga gcgaagttga
    28921 taccaaacaa cttgccccag aacttggtca tatctttata aatctgtttg ccggagagga
    28981 cgtagaccgt ttccataatg gccagcagga acgccatacc gagcgtcagt ggcacaaaaa
    29041 ggaagtggta catcgcggtc aaggcaaact gtaagcgcga cagttcgact atatctaaca
    29101 tcatgactcc ttgctcatcg catgaagact ccgagagtga accccgttag aaagggtcac
    29161 acgcatgccc caatagaaat ttgtttgaac actttcatca taccatgtga atgcacacaa
    29221 aaacggcgta aagaaaggtt gcaaacatgt taataaaaac tcaaattgat cccacgtata
    29281 tattacgccg caaaatcctt acaataaaca ggtttttgtt gaagcaaatt tgcatttttc
    29341 gacggtgatc aatttatagc taaattaccg cctttcagcc aatttgatcg agaacaattt
    29401 atctcttttt gatgcccatt t
//
LOCUS       DAZGVQ010000053        29413 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000053 DAZGVQ010000000
VERSION     DAZGVQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29413
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.053"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..366
                     /locus_tag="ACHLAG_002209"
     CDS             <1..366
                     /locus_tag="ACHLAG_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP0655434.1"
                     /translation="PFNQMKNPTDPEELANIFNKKHKEHMDDRFIHLIRSNAIASTPV
                     YDNYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLGVDQHQKIDNYTMGLNSWKIA
                     PNERGDKKGVPGLSCFQPH"
     gene            628..2250
                     /gene="yhiJ"
                     /locus_tag="ACHLAG_002210"
     CDS             628..2250
                     /gene="yhiJ"
                     /locus_tag="ACHLAG_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP0655435.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMRAHILRR
                     DNNYQFIVTINSKNYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPVWDWK
                     ISQFNVSDIKFETMINPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLRIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYAGIKDYPVCFYDPKYKIMANHHGITFDEQRKRYIIGPIT
                     VSIDEMTNALDPVELAAIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            2440..2553
                     /gene="yhiY"
                     /locus_tag="ACHLAG_002211"
     CDS             2440..2553
                     /gene="yhiY"
                     /locus_tag="ACHLAG_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFP0655436.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            2616..3683
                     /gene="yhiI"
                     /locus_tag="ACHLAG_002212"
     CDS             2616..3683
                     /gene="yhiI"
                     /locus_tag="ACHLAG_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP0655437.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAHRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAELGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            3680..6415
                     /gene="rbbA"
                     /locus_tag="ACHLAG_002213"
     CDS             3680..6415
                     /gene="rbbA"
                     /locus_tag="ACHLAG_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFP0655438.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVIHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIVRGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6415..7539
                     /gene="yhhJ"
                     /locus_tag="ACHLAG_002214"
     CDS             6415..7539
                     /gene="yhhJ"
                     /locus_tag="ACHLAG_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0655439.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQKIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            7643..7873
                     /locus_tag="ACHLAG_002215"
     CDS             7643..7873
                     /locus_tag="ACHLAG_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFP0655440.1"
                     /translation="MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMS
                     VACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            7870..8229
                     /locus_tag="ACHLAG_002216"
     CDS             7870..8229
                     /locus_tag="ACHLAG_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP0655441.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYVRTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(8349..8750)
                     /gene="nikR"
                     /locus_tag="ACHLAG_002217"
     CDS             complement(8349..8750)
                     /gene="nikR"
                     /locus_tag="ACHLAG_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFP0655442.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(8759..9562)
                     /gene="nikE"
                     /locus_tag="ACHLAG_002218"
                     /pseudo
     CDS             complement(8759..9562)
                     /gene="nikE"
                     /locus_tag="ACHLAG_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
     gene            complement(9559..10323)
                     /gene="nikD"
                     /locus_tag="ACHLAG_002219"
     CDS             complement(9559..10323)
                     /gene="nikD"
                     /locus_tag="ACHLAG_002219"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFP0655443.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(10323..11156)
                     /gene="nikC"
                     /locus_tag="ACHLAG_002220"
     CDS             complement(10323..11156)
                     /gene="nikC"
                     /locus_tag="ACHLAG_002220"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFP0655444.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(11153..12097)
                     /gene="nikB"
                     /locus_tag="ACHLAG_002221"
     CDS             complement(11153..12097)
                     /gene="nikB"
                     /locus_tag="ACHLAG_002221"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFP0655445.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(12097..13671)
                     /gene="nikA"
                     /locus_tag="ACHLAG_002222"
     CDS             complement(12097..13671)
                     /gene="nikA"
                     /locus_tag="ACHLAG_002222"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0655446.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(13782..14369)
                     /gene="acpT"
                     /locus_tag="ACHLAG_002223"
     CDS             complement(13782..14369)
                     /gene="acpT"
                     /locus_tag="ACHLAG_002223"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFP0655447.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(14424..15473)
                     /gene="yhhT"
                     /locus_tag="ACHLAG_002224"
     CDS             complement(14424..15473)
                     /gene="yhhT"
                     /locus_tag="ACHLAG_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP0655448.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            15605..16822
                     /gene="yhhS"
                     /locus_tag="ACHLAG_002225"
     CDS             15605..16822
                     /gene="yhhS"
                     /locus_tag="ACHLAG_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0655449.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     SVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(16826..17383)
                     /gene="dcrB"
                     /locus_tag="ACHLAG_002226"
     CDS             complement(16826..17383)
                     /gene="dcrB"
                     /locus_tag="ACHLAG_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFP0655450.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(17456..18121)
                     /gene="yhhQ"
                     /locus_tag="ACHLAG_002227"
     CDS             complement(17456..18121)
                     /gene="yhhQ"
                     /locus_tag="ACHLAG_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFP0655451.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            18342..18587
                     /gene="tusA"
                     /locus_tag="ACHLAG_002228"
     CDS             18342..18587
                     /gene="tusA"
                     /locus_tag="ACHLAG_002228"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFP0655452.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(18689..20887)
                     /gene="zntA"
                     /locus_tag="ACHLAG_002229"
     CDS             complement(18689..20887)
                     /gene="zntA"
                     /locus_tag="ACHLAG_002229"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFP0655453.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQATDEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(20961..21587)
                     /gene="yhhN"
                     /locus_tag="ACHLAG_002230"
     CDS             complement(20961..21587)
                     /gene="yhhN"
                     /locus_tag="ACHLAG_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFP0655454.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            21728..22087
                     /gene="yhhM"
                     /locus_tag="ACHLAG_002231"
     CDS             21728..22087
                     /gene="yhhM"
                     /locus_tag="ACHLAG_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFP0655455.1"
                     /translation="MSKPPLFFIVIICLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFSLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(22090..22359)
                     /gene="yhhL"
                     /locus_tag="ACHLAG_002232"
     CDS             complement(22090..22359)
                     /gene="yhhL"
                     /locus_tag="ACHLAG_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFP0655456.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(22349..22945)
                     /gene="rsmD"
                     /locus_tag="ACHLAG_002233"
     CDS             complement(22349..22945)
                     /gene="rsmD"
                     /locus_tag="ACHLAG_002233"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFP0655457.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            23095..24591
                     /gene="ftsY"
                     /locus_tag="ACHLAG_002234"
     CDS             23095..24591
                     /gene="ftsY"
                     /locus_tag="ACHLAG_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFP0655458.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVAEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            24594..25262
                     /gene="ftsE"
                     /locus_tag="ACHLAG_002235"
     CDS             24594..25262
                     /gene="ftsE"
                     /locus_tag="ACHLAG_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFP0655459.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            25255..26313
                     /gene="ftsX"
                     /locus_tag="ACHLAG_002236"
     CDS             25255..26313
                     /gene="ftsX"
                     /locus_tag="ACHLAG_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFP0655460.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            26558..27412
                     /gene="rpoH"
                     /locus_tag="ACHLAG_002237"
     CDS             26558..27412
                     /gene="rpoH"
                     /locus_tag="ACHLAG_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFP0655461.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            27683..28786
                     /gene="livJ"
                     /locus_tag="ACHLAG_002238"
     CDS             27683..28786
                     /gene="livJ"
                     /locus_tag="ACHLAG_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFP0655462.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(28985..29368)
                     /gene="panM"
                     /locus_tag="ACHLAG_002239"
     CDS             complement(28985..29368)
                     /gene="panM"
                     /locus_tag="ACHLAG_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFP0655463.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPAVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         7121 a         7520 c         8029 g         6743 t
ORIGIN      
        1 ccatttaatc agatgaaaaa tcctactgat cctgaggaat tagccaatat ttttaataaa
       61 aaacataaag aacacatgga tgatcgtttt atccatctaa ttagaagcaa tgctatagca
      121 tccacacctg tatatgacaa ttattttaat aacactactg ctttcagacc aaaacctgaa
      181 gatatcttca aatgtggaca gacgctcaaa aaaactaaac agaaatatgg ccattatgga
      241 ttaggggtag atcagcatca aaaaattgat aattatacca tggggctaaa ttcctggaag
      301 atagctccta acgaacgtgg tgataaaaaa ggtgtcccag ggcttagttg ttttcaacca
      361 cattaaaact ccatgagtgc tgtggtatat ctttttaatc ctgccagtat ttcataggtt
      421 ttcttaatcg attgtagagg catgttctga tgacgcctct acaatcttta gggactttct
      481 ctgctatgag ataagaaatt tcatattatg ctttaaggct gaatgatttg tgcaaaatat
      541 aattattttt ccatcgaaca tgaggtgtat gtcataagat taaatgttaa ctatgcattc
      601 ctcgcggtga attatatgag gtaaaagatg aaaattggaa ctgtggcagg cactaacgac
      661 agtacaacga caatcgcaac aaatgatatg gtgcaagagc atgttactaa cttcactaaa
      721 gaactgtttg ggtatattgc aaatggtata ggtgatgata tatcaagtat tgccaggaca
      781 atgctcggtg aagttgtgga aaaaattgat gattggcaaa tagaacgttt ccaacaatct
      841 attcgagatg acaaaatatc atttaccata caaacgaatc attcagaaaa atatagtatg
      901 ctatcggaca tgcgcgctca tattctacgt agggataata attatcagtt tattgtgaca
      961 attaatagta aaaattacgg ctgctatctt gataataccg atattaattg gtgcagcatt
     1021 gtttatttac ttaataatat gacagtaaat gatgatgcta atgatgttgc agttactgag
     1081 tcgtacaaac ccgtctggga ctggaaaata tcgcaattca atgtatctga tattaaattc
     1141 gaaacaatga taaaccccca gttcgctgac agaacctatt tctccaattg ttcacctgtt
     1201 gatcccacaa gtaccagacc aacctatttt ggcgataccg atggctcagt cggtgcagtt
     1261 ttgtacgcgc tttttgcaac gggacatttg agaatcatgg ctgaaggaga aaatttcctt
     1321 agccagttgc ttaatataga agatgaagtt ctcaacgtat tgctaaggga gaactttaat
     1381 gagcaacttg acactaatgt taatacaata atcagtattc tgaacagaag agatatagtt
     1441 ttagaaagtc ttcagcctta tttagttatt aataaagacg ctgtgacgcc atgtacgttt
     1501 cttggcgacc aaaccggcga tcgatttagt aatatctgtg gcgatcaatt tatcatcgat
     1561 ttgttaaaac gcataatgag tatcaatgaa aatgttcatg tattagctgg aaatcatgaa
     1621 acgaattgca atggtaacta tatgcagaac ttcacgcgta tgaaaccact tgatgaggat
     1681 acctacgctg ggataaaaga ttatcctgta tgtttctatg accccaaata caaaataatg
     1741 gcaaatcatc atgggataac ttttgatgag cagcgaaaac gttacattat aggaccgata
     1801 acagtctcta ttgatgaaat gaccaatgct ctcgatccgg tcgaattagc ggcaattatc
     1861 aataaaaagc atcatgctat aattaatggc aaaaagttta aaactagccg ggctatctct
     1921 tgtcgatcct ttaatcgtta tttttctgtt tcgacagatt acagacctaa acttgaagca
     1981 ctattagcat gttcacaaat gttaggtata aatcaagttg ttgcacataa cggaaatggt
     2041 ggaagagaac gcataggcga aacggggaca gttttaggtc ttaacgcccg tgacagtaaa
     2101 catgctggaa gaatgttcag catgcataat tgccaaatta accctggtgc agggcctgag
     2161 ataaccactc cctggaagtc ttaccagcat gaaaaaaaca gaaatggact tatgcctttg
     2221 attagaaggc gcacaatgtt acagctctga accatagaca acatgaatat taatgttact
     2281 atctggttga gactgttgat aaaacgtaaa aaggaatgct ctgttcctgc aactgaacag
     2341 gcgtgaaacg ctatccaaca ggatggatac cgttttgtca gccagtttta tagatcacac
     2401 ttatgagtat gcgtgtttca tttacggcca ggaatgctga tgatgacaac attactaccc
     2461 gttttcacta agccttcccc gttggcgctc aatgctctgc gcgctggtcg tatttgccgt
     2521 ttccttctta tcccggatgg aagaatccgc tgaacgtcgt tcggcggcag ttgtaacacg
     2581 cgtcgtttgc ttacagggat tatggcgggt cgattatgga taagagtaag cgccatctgg
     2641 cgtggtgggt tgtcgggtta ctggcggtgg cggctatcgt ggcgtggtgg ctgttgcgcc
     2701 cggcaggtgt gccggaaggc tttgctgtca gcaatgggcg cattgaagcg acggaagtgg
     2761 atatcgccag caaaattgcc gggcgtattg acaccattct ggtgaaagaa ggccagtttg
     2821 ttcgagaagg tgaagtgctg gcgaagatgg atactcgcgt gttgcaggaa cagcgactgg
     2881 aagccatcgc gcaaatcaaa gaagcacaaa gcgccgttgc tgccgcgcag gctttgctgg
     2941 agcaacgcca aagtgaaact cgcgccgctc agtcgctggt taatcaacgg caagctgaac
     3001 tggactccgt agccaaacgt catacccgtt cccgctcgct cgcccatcgt ggggctattt
     3061 ctgcgcaaca gttggatgac gaccgcgccg ccgctgagag cgcccgagct gcgctggaat
     3121 cggcaaaagc tcaggtttcg gcttctaaag ccgctataga agcggcacgc accaatatca
     3181 ttcaggcgca aactcgcgtc gaagccgcac aagccactga acggcgcatt gccgcagata
     3241 tcgatgacag cgaactgaaa gccccgcgtg acggacgcgt gcagtatcgg gttgccgagc
     3301 taggcgaagt gctggcggca ggcggtcggg tgctgaatat ggtcgatctc agcgacgtct
     3361 atatgacttt cttcctgcca accgaacagg cgggcacgct gaaactgggc ggtgaagccc
     3421 ggctgatcct cgatgccgcg ccagatctgc gtattcctgc aaccatcagt tttgtcgcca
     3481 gtgtcgccca gttcacgcca aaaaccgtcg aaaccagcga tgaacggctg aaactgatgt
     3541 tccgcgtcaa agcgcgtatc ccaccggaat tactccagca gcatctggaa tatgtcaaaa
     3601 ccggtttacc gggcgtagcg tgggtgcggg tgaatgaaga acttccgtgg cctgacgacc
     3661 tcgtggtgag gttgccgcaa tgacgcatct ggaactggtt cccgtcccgc ctgtcgcgca
     3721 actggcgggc gtgagccagc attatggaaa aaccgttgcg ctgaacaata tcacactcga
     3781 tattccggcc cgttgcatgg tcgggctgat tggcccggac ggcgtcggga agtcgagctt
     3841 gttgtcgttg atttccggtg cccgcgtcat tgaacagggc aatgtgatgg tgctgggcgg
     3901 cgatatgcgc gacccgaagc atcgccgcga cgtctgcccg cgcatcgcct ggatgccgca
     3961 ggggctgggc aaaaatctct accacacctt atcggtgtat gaaaatgtcg attttttcgc
     4021 tcgcttgttc ggtcacgata aagcggagcg ggaagtccga attaatgagc tgctgaccag
     4081 caccgggtta gcaccgtttc gcgatcgtcc ggcaggtaaa ctctccggcg ggatgaagca
     4141 aaaactgggg ctgtgctgcg cgttaatcca cgacccggaa ctgttgatcc ttgatgagcc
     4201 aacaacgggg gttgacccgc tctcccgcgc ccagttctgg gatctgatcg acagtattcg
     4261 ccagcggcag agcaatatga gcgtgctagt cgccaccgcc tatatggaag aggccgaacg
     4321 cttcgactgg ctggtagcga tgaatgccgg agaagtgctg gcaactggca gcgccgaaga
     4381 gctgcggcag caaacgcaaa gcgccacgct ggaagaggcg tttataaatc tgttaccgca
     4441 agcgcaacgc caggcgcatc aggcggtagt gatcccaccg tatcaacctg aaaatgcaga
     4501 gattgccatc gaagcgcgcg atctgaccat gcgttttggt tccttcgttg ccgttgatca
     4561 cgtcaatttc cgcattccac gcggggagat ttttggtttt cttggatcga acggctgcgg
     4621 taaatccacc accatgaaaa tgctcaccgg actgctgccc gccagcgaag gtgaggcgtg
     4681 gctgtttggg caaccggttg atccaaaaga tatcgatacc cgccgtcggg tgggctatat
     4741 gtcgcaggcg ttttcgctct ataacgaact caccgtgcgg caaaaccttg agttacatgc
     4801 ccgtttgttt cacatcccgg aagcggaaat tcccgcgcga gtggctgaaa tgagcgagcg
     4861 ttttaagctc aacgacgttg aagatgttct gccggagtca ttgccgctcg gcattcgcca
     4921 gcggctttcg ctggcggtgg cggtgattca tcgcccggag atgttaatcc tcgatgaacc
     4981 tacttctggc gtcgatccgg tggcgaggga tatgttctgg cagttgatgg tcgatctctc
     5041 gcgccaggac aaagtaacca tttttatctc cacccacttt atgaacgaag cggaacgttg
     5101 cgaccgcatc tcactgatgc acgccggaaa agtgctcgcc agcggtacac cgcaggaact
     5161 ggttgagaaa cgcggagccg ccagtctgga agaggcattt atcgcctatt tgcaggaagc
     5221 ggcaggacag agcaacgaag ccgaagcgcc gcccgtgata cacgacacca cccacgcgcc
     5281 gcgtcaggga tttagcctgc gccgtctgtt tagctacagc cgccgcgaag cactggaact
     5341 gcgacgcgat ccggtacgtt cgacgctggc gctgatggga acagtgatcc tgatgctgat
     5401 aatgggttac ggcatcagta tggatgtgga aaacctgcgc tttgcggtgc tcgaccgcga
     5461 ccagaccgtc agtagccagg cgtggacgct caatctctcc ggttcccgtt actttatcga
     5521 acagccgccg ctcaccagtt atgacgagct tgatcgtcgg atgcgtgcgg gcgatataac
     5581 ggtggcaatt gagatccctc ccaatttcgg gcgcgatatc gtgcgtggta cgcctgtgga
     5641 actcggcgtc tggatcgacg gagcgatgcc gagccgtgcc gaaacggtaa aaggttacgt
     5701 gcaggcaatg caccagagct ggctacagga tgtggcgagc cgacaatcca cacccgccag
     5761 ccaaagcggg ctgatgaata ttgagacgcg ctatcgctat aacccggacg taaaaagcct
     5821 gccagcgatt gttccggcgg tgatcccgct tctgctgatg atgatcccgt caatgctaag
     5881 cgcccttagc gtggtgcggg aaaaagagct tgggtcgatt atcaaccttt acgtgacccc
     5941 caccacgcgt agtgaatttt tgcttggtaa acagttgcca tacatcgcgc tggggatgct
     6001 gaactttttc ctgctctgcg gcctgtcggt gtttgtgttt ggcgtaccgc ataaaggcag
     6061 tttcctgacg ctcaccctgg cggcgctgct gtatatcatc attgccaccg gaatggggct
     6121 gctgatctcc acctttatga aaagccagat tgccgccatt ttcggaacgg cgattatcac
     6181 gttgatcccg gcgacacagt tttccgggat gatcgatccg gtagcttcgc tggaagggcc
     6241 tggacgttgg atcggcgagg tttacccgac cagtcatttt ctgactatcg cccgcgggac
     6301 gttctcgaaa gcgctggatc tgactgattt gtggcaactt tttatcccgt tgctgatagc
     6361 catcccgctg gtgatgggct taagtatcct gctgctgaaa aaacaggagg gatgatgcgc
     6421 catttacgca atatttttaa tctgggtatc aaagagttgc gcagtctgct cggtgataaa
     6481 gcgatgctga cgctgattgt cttctcgttt acggtgtcgg tgtattcgtc agcgaccgtt
     6541 acgccaggat cgttgaacct cgcgccgatc gccattgccg atatggatca atcgcagtta
     6601 tcgaaccgga tcgttaacag cttctatcgt ccgtggtttt tgccaccgga gatgatcacc
     6661 gccgatgaga tggatgccgg actggacgcc ggacgctata ccttcgcgat aaatattccg
     6721 cctaattttc agcgtgatgt cctcgccgga cgccagccgg atattcaggt gaacgtcgat
     6781 gccacgcgca tgagccaggc atttaccggc aatgggtata tccagaagat tatcaacggt
     6841 gaagtgaaca gctttgtcgc gcgctaccgt gataacagcg aaccgttggt atcgctggaa
     6901 acccggatgc gctttaaccc gaacctcgat cccgcgtggt ttggcggggt gatggcgatc
     6961 atcaacaaca ttaccatgct ggcgattgta ttgaccggat cggcgctgat ccgcgagcgt
     7021 gaacacggca cggtggaaca cttactggtg atgccgataa cgccgtttga gatcatgatg
     7081 gcgaagatct ggtcgatggg gctggtggtg ctggtggtat cgggattatc gctggtgctg
     7141 atggtgaaag gtgtactggg cgtaccgatt gaaggctcga tcccgctgtt tatgctgggc
     7201 gtggcgctca gtctgtttgc caccacgtca atcggcattt ttatggggac gatagcgcgt
     7261 tcaatgccgc aactggggct gctggtgatt ctggtgctgc tgccgctgca aatgctttcc
     7321 ggtggatcca cgccgcgcga aagtatgccg cagatggtgc aggacattat gctgaccatg
     7381 ccgacgacac actttgttag cctcgcgcag gccattctct accggggtgc cggattcgaa
     7441 atcgtctggc cgcagttcct gacgctgatg gcaattggcg gcgcgttttt caccatcgcg
     7501 ctgctgcgat tcaggaagac gattgggaca atggcgtaag gaagggaaac ttgacttcat
     7561 actgtgatgt cataaagtga agtcataagg gaatgtcatg taaacacagg tacaggcttt
     7621 acgaaagaaa caaaaaaaca cactggatca gctatttaaa tcacctttac ctcaaggaat
     7681 caaatggact gatatagaat ctctggttaa agcgttaggc ggagaaatta aggaaggtag
     7741 ggggtcgcgc tgtaagttca tactaaatat gagcgttgcg tgtttccatc ggcctcatcc
     7801 gtcgccagat accgataaag gcgctgtaga aagcgtgcgt gactggttac taagtatagg
     7861 ggtaaaacca tgatcaaact gaagacgcca aactctatgg agattgccgg gcaacctgct
     7921 gttatcactt acgtgccaga actgaatgca tttcgtggca aattcctggg cttgtcaggt
     7981 tattgtgatt ttgtctctga cagcattcag gggctgcaaa aagaagggga actttccctg
     8041 cgagagtatc ttgaggattg taaagctgcg ggtattgagc cgtatgtgcg tacagaaaag
     8101 attaagacct ttacgttacg ctatccagaa tcgctgagtg aacgccttaa taatgcggct
     8161 gcgcagcaac aagtctctgt taatacctat attattgaaa cactgaatga gcgtttgaat
     8221 catttataaa ttttagcggc gtacattacg cagaatatct gcctttacag gatctatcta
     8281 tgcaactgaa cttaatttta taaattaagt tcagttaata tcttttcttt cctcgtttca
     8341 gaaataactc aatcttcctt cggcaggcac tgcaaatgcc cgtgccgcac gccgcgctgg
     8401 gcgataacgt catcggcaaa atgctgcacg tcgcccatat cacctttcag cacggcgatt
     8461 tccagacagt cgtcgtgatt gatatgcaca tgcagcgtgg cgacggagag atcgtggtga
     8521 tggtgctggg tggagacaat gcggctggct aagtcgcgtt tttcgtgttc atacacatag
     8581 gagagcaccg cgaaaccttg cgtgccgtgc tgctgagtgg cctcctgggc cagggcgcta
     8641 cgcagaatat cgcggatagc ttcggaacgg ttgttataac cacgacgctg gctcaggctg
     8701 tccagcgtct ccagtaaatc gtcatcaagc gtgatggtga ctcgttgcat ttgcgtttaa
     8761 ccttttctgt ggtgcgacgg cgcacgggga atgcgggtaa taccgcgttt tgtagcacac
     8821 gtccggcgtc agaggaaaag gttaatttat ctcccaccac ctgggtttcg acgatttgtc
     8881 cgttgtccat caccatcacc cgctgacaga agcgttccac caggcgtaag tcgtgggtga
     8941 tgaacaggca ggcgctgcca aactgttgtt gtagcttttt cagcaggcga atgacgcccg
     9001 cctgtaacac gagatcaagg ttagaaacgg cttcatccag aatcagtagt ttcggttcga
     9061 ccgccagcgc gcgagccagg cagacgcgct ggagctggcc gccgcttaac tgcggtgggc
     9121 gtttgtcgag aacgctgtca tcgagatcga ccgccttcag catttcgctg gcgcgcgcca
     9181 gttgttcgga ttttttcagt gatagcaggt ggcgcatcgg ttcacgcagg atctcgcgca
     9241 cggttttgcg cggattcacg gcgctgatgg agtcctgaaa taccatctga atatcgcggc
     9301 ggaacgcttt acgctgggcg cgattgagtt tcgccagcgg ttcgccgcgc cagctaatat
     9361 tcccttgtga gggagactct aagcctacca gcagccgcgc gagggtgctt tttccgcagc
     9421 cgctgcgtcc cagcagggcg acggtttcgc cgcttttcag ggtcagggaa acgttattca
     9481 gcaccgcctg atgttgatgt tttccgctaa atccaccgtg ggcatagtga tgggaaaggc
     9541 cgcagacgtt aagtaaggtc atgatgccag ctccataccg tagagggcga gatgagcgga
     9601 aaccaggctg cgcgtcaccg tatgtttggg ggcgttaaac agcgtttcta catcgccctg
     9661 ttcgacaatt ttaccttgtg acatcaccgc cacgtcatcc gccagacgcg ccacaacgcc
     9721 catatcatgg gtcaccagca gcattcccgg cgcttgtttt tgcataatgc tttccagcag
     9781 atcgaggatg cgcgcctgtg ctaccacgtc gaggtcggtg gtcggttcat cggcgatgat
     9841 aaacggtgat tcacacagca ccgccatcgc aatcatcatg cgctgcaaca tgccgccgct
     9901 catctcgaac gggtacagct tcagcacgcg cgcggcgttt tccagcccca ccgcttctat
     9961 ggcagcggta agcgtagcgt catcggcggg tttccctaac gccaggcagg tttcccgcgc
    10021 gtgggtgtgc atggtgtgca gtggattaaa ggcgctgcgc gggttctgca tgatggtggc
    10081 aattttgatg ccgcgcaggg cgcaaggcga aaccggtttg ccatcggcta aaatttcccc
    10141 cgccgtctgg cgaacgccag cgggcaaaat gcccagcgtc gcggcgcagg ttaatgattt
    10201 cccgctgccg ctaccgccga ctaacgccag cacgcgcccg cgttgcaggg ttaacgatac
    10261 accgtgtacc agcggctgcg cggcctgtag cgcgatatta cgtagttcaa tctgttgcgg
    10321 cattagtgtg cgtgctccgt caccagatga ggatccagat gatcgcgcag tgcgtcaccc
    10381 accaggttaa aggccatcac gctgataaac agcgccagcc ccggccagaa catttgcagc
    10441 ggctgggtcc agatatactg gcgcgcgtcg ttaatcatca cgccccattc ggcggtcggc
    10501 gcggtcacac cgaggccaag gaaagacatc cccgcgacgt gcagcatcat atggccgata
    10561 tccagcgttg ccagcaccag cagcgaaggg atcaccgcgc ctgccagatg atcgacaaac
    10621 acccgcacat ggcccgcacc ggaaagccgt gacgccagca caaactcgcg ttggcgtagt
    10681 gaaatcacca ggctgcgcac catgcgtgca taccacgccc agtgcgacag ggcgatggcg
    10741 ataattacgt tggtcagccc ggtgccgagc acgccaacca taaagaacga cagaatcgag
    10801 gtcgggaagg tcataaacat atcggcgacg cgcatggtgg cctgatcaac gcgtccgcca
    10861 atcagtccgg cgctgccgcc aatcaccagc cctaatgtca gcaccagcag caggcaggcc
    10921 attaccgaac cgagcgacac gcgggtcgcc gccatcagcc gcgagaaaat atcacgccct
    10981 aagtgatcgg tgcccagcca gtgctgcgca tccggcgaaa gcaggcgcga cggcagatca
    11041 atagcctgag ggtcatacgg cagccaccac tggctggtaa gcgcaatcag cgccagcagg
    11101 gcgataatga tcagcgccag gcgtaccgac cagcgggaag agaggaaaaa gttcacgcgt
    11161 gcgctccttc atgacgacga atgcgcgggt ccagcgcggc gttgagcaaa tcgacaatca
    11221 aattacagac cacaaaaacc accaccatca tcagcgtaaa gcactggatc accggatagt
    11281 cacggttaaa aatcgccgac accgcatagc gcccgacgcc cggccaggca aagatgtttt
    11341 cgataatcat cgtcccgccg atcagttcgc cgatgtgcat ccccacggcg gtgatcatcg
    11401 gcagcgaggc attgcgcagg atgtggcgac gttcggtctg tttgtcgttc aggccgcgca
    11461 gacgcgccca ggtgacgtga cgctgaccgg cgacgtccag catactggcg cgcagtaaac
    11521 gcgcgttaat cgccagcgac ataaaggcaa tggaaaccgc aggcaaaatg atgtgctgcc
    11581 agccgccgta acccatcgcg ggtagccatt gcagatacac cgaaaacgcc atcaccagta
    11641 aaaacgccag ccagaagtta ggcatcgaca cgccaagaaa cgcgatgaaa cgtacggcga
    11701 aatccggcag acggtcgcgg tggcgcgccg cccagatacc gagcggtacg gaagtgagca
    11761 gaattaatac cagcgccgca cctgcaagtt ccagcgtggc gggcaggaag ttcagcatat
    11821 cgtccagtac cgggcgttgg ctggcgaatg agataccaaa gtcaagatgc agcgccttcc
    11881 acaaccaggt gccgtactgg acgtacagcg gctgatccag tcccagcatg gtgcgggtag
    11941 aggccagcat ctccggcgtc ggcggcaggt tagacagacg caaataatcg agcgccgggt
    12001 cgccggtacc gaggcgcagc atcagaaaaa tgatcaccga ggcggcgagc accatcggga
    12061 tcagcagcag aaagcggcgt aatacgtaac gcaacattaa ggtttcaccg gtttaatctg
    12121 ttcgaacgga atttcggtgg cgatcggcgc gtaggggata ttacccagct ccggttttga
    12181 taccaccatc atcgagatgt agctgatagg cagataaacc gcctcgtcat gcagacgggt
    12241 cagtatgtcg cgatacagcg cctgacgttg cgtttcgtca tgggtcgcca gcacttcgcc
    12301 gatctcttta tcaatcagcg gcttatcggc taatccttgc tgtgcctgga agtcagcgtg
    12361 cgacggtacg cgcattgaac tcaggaaggc gtgtggatcg tatggcgcgc cccaggtgcg
    12421 gtggaaaatc atgccaaaac gaccgtcgcg ctgacgagca tagatactgc tctcttcttc
    12481 gccaatcagc gagacatctg cgccaatctg gcgcatatca gcctgaatga tttccgccat
    12541 cgatttgctt aacgcatcgg tgccgatgaa cgacagttca atgcgcagcg gctgaccatt
    12601 tttctcgcgg atgtctttgc ccgcaggcag cgtccaaccg gctttttcca gcaacgcttt
    12661 cgctttctgc ggatcgtact ggcgcggttt caggccgaga ttagcgtaag gcacagaagg
    12721 ggcaaacagg gtgtcggcga cctgctgggt gccatataac gcgttatcaa tcagcgattt
    12781 tttgtttacc gcgtaattaa gagcttcacg tactgccagc tcgttggtgg gggctttggc
    12841 ggtattgagc gccagcatca cggtttcgat cggctgtgac agttgggtgt ggtaagccgg
    12901 attctggcta aagcgggcga aggtatcgag cggtaataac ccttcgtttc cgtacagcag
    12961 gtcgatatcg ccagtttcaa acgccaccgc gcgggtagtc gggtccggga tgacgttaaa
    13021 ggtgatcttt ttaatcgctg gcttttcgcc ccagtagttt tcgttacgga cgaagacatc
    13081 gtactgattc agtttcgatt cctgcaaaat ccatggtccg gtgccaatcg gcgctttaat
    13141 tccgttcatg gtttcatggt ttttaaactg cgagggagcg ataaaacgga aaggacgggg
    13201 cagggccagt tcttgcagga aaggatagta ggcgcttttc agggtaattt gcagctctgt
    13261 tttactgagt gctttaacat caacaatctg gtttgccagc tccagccagg cgtgacgttg
    13321 acggttatcg agcactgcgc ggaagttttc tgccgccgcc tcggcatcga acggttcacc
    13381 gttggagaat ttcacgtcat cacgcagggt gaaggtccag gttttaccat cttctgaatg
    13441 agtccagctt tttgccagcc acgggatcac cgaaccgtct gcctgatatt tcaccaatgg
    13501 ttcataaacc atgctctggg cgaacatctg gttaggcgtg taaaggtgcg ggtttagtgg
    13561 cccgacattc accggccagg cggtagtgat ttcatctggt gcagcggcat ggacgataaa
    13621 agacgcacaa gccagcagcg caaatagagt gcggcggagt gtggagagca tgataacccc
    13681 aatggattaa aatagatggc ggaaataagt atgacgattt taagtattcg tcatactgat
    13741 tacctgttct ggatcaatag atgggcagtc gggtgggcgg atcagttaac tgaatcgatc
    13801 cattgcacac tgtcggcggt gagcgtaaag ggggtggggg tgcagatcgc caggcttaaa
    13861 ttttcaagct gacaatggct gactgacagc gaggagtgat aggtgctgtc tacgctgacg
    13921 atttgccagg cgctgccgcc acgctgttta acgatggctt ctttgcgcgt ccagatgcgc
    13981 cagaacattt ccagttgctg atcaggatgc acggcgtcca tctcagcgtg ttccccgagg
    14041 ctgaatacgg cgttcgccag ccagcgccag ttggcgcgcg ggcgaatcac ttcgatatcg
    14101 cagccgactt cgccttcatc actcaacagc agagcgatat cgtcaccgct atggcttaag
    14161 ttgaaccata gcggcgtttc cggcgcgaat gcaggtttgc cttgttcgcc atagatgatc
    14221 tccggtagcg gggaaagcgt gtgcgaaagc aatgcacgcc ccgccagcca gcgttcgcgt
    14281 cgtggacctt gcggtgcttg ctcgcgtaaa cccggtggca gtggagctgc gcttaaggtc
    14341 gaaactttcc ccagaactat ccgatacata tcagggccaa cgtttaatgg aaaatgaaag
    14401 tgcgtatcgt atcacttgtc gcctcatccc ggtaaccgac ttttcggtct gcccggcccc
    14461 agtaaaatcg ccagtttgct gccacctttc gtcgtttcca tccagatttt acacacgctg
    14521 gttaatggca ccgaaagtag catccctacc gggccgagca gccatcccca gattaacaat
    14581 gaaagaaata ccaccatggt ggacatcccc aggcgatggc ccatcatccg tggctctaaa
    14641 atattgccga tgaccatatg gacaactaaa aacaatgcgc cgaccagaat acattcgtaa
    14701 ataccattaa acagcagcac ctgaatcatt ggcggtacgg cggaaattac cgcgccgata
    14761 ttgggcacgt agttaagcaa aaacgccagt actgcccaca tcagcgcaaa ctgtaccccc
    14821 atcagctcca gccccagcca gacgatgaca cctgtccata aactgagtag cgtcttcaat
    14881 gcaagatagt gcgaaacgcc tttaagtgcg cggtgtaatc ccgcgatgtg aatctgtgga
    14941 ttattcagcg caaaacgcat tttgtaaggg acgtggcgca cttcaaacag cataaaaact
    15001 acggtcatca ccagcaaaag cacgctcgcc attgccccgg aaagcccggt cattagcgct
    15061 gtggtgaagg taaccacttt ttccgagtcc atccgctgca gcattcgctc cggcgacata
    15121 tgcaaattaa gaaaaggcaa catctcctgc aatttaaaaa gtttgcgcgt cagctcctta
    15181 ttaaacttcg gcagcataga gataaattcg ttaaacgatg ccgccagtac gccgaccagc
    15241 gcggttagtg cgatcagcat caccaccact acaatcgtaa tggcaacggg gcgttgtact
    15301 ccccgacgaa taaaccaggt gacgagcggg ttgaggacga tggcaaaaaa cagcgccagt
    15361 aacagctgca caatgatatc tgccgctgcg tgaatgcccg cgaggatcac taccagcgag
    15421 gccagcttga gcagaatgtg catgcccgtt ttatcgggtt gaggggtttc cattggggct
    15481 tccttgtgac tttttgtatt aagtgtagcg ggagtcacgc cagcaatatt ctgattctta
    15541 ctgctgattt tcacgtcagc acatggtaaa aatgaaacac tgttgtaaaa atgtggtgat
    15601 cctcatgccc gaacccgtag ccgaacccgc gctaaacgga ttgcgcctga atttgcgcat
    15661 tgtctccatt gtcatgttta acttcgccag ctacctcacc atcgggttgc cgctcgctgt
    15721 attaccgggc tatgtccatg atgtgatggg ctttagcgct ttctgggcag gattggttat
    15781 cagcctgcaa tatttcgcca ccttgctgag ccgccctcat gccggacgtt atgccgattt
    15841 gctgggaccc aaaaagattg tcgtcttcgg tttatgcggc tgctttttga gcggtctggg
    15901 gtatctgacg gcaggattaa ccgccagtct gcctgtcatc agcctgttat tactttgcct
    15961 ggggcgcgtc atccttggga ttgggcaaag ttttgccgga acgggatcga ccctgtgggg
    16021 cgttggcgtg gttggctcgc tgcatatcgg gcgggtgatt tcgtggaacg gcattgtcac
    16081 ttacggggcg atggcgatgg gtgcgccgtt aggcgtcgtg ttttatcact ggggcggctt
    16141 gcaggcgtta gcgttaatca ttatgggcgt ggcgctggtg gccattttgt tggcgatccc
    16201 gcgtccgacg gtaaaagcca gtaaaggcaa accgctgccg tttcgcgcgg tgcttgggag
    16261 cgtctggctg tacggtatgg cgctggcact ggcttccgcc ggatttggcg tcatcgccac
    16321 ctttatcacg ctgttttatg acgctaaagg ttgggacggt gcggctttcg cgctgacgct
    16381 gtttagctgt gcgtttgtcg gtacgcgttt gttattccct aacggcatta accgtatcgg
    16441 tggcttaaac gtagcgatga tttgctttag cgttgagata atcggcctgc tactggttgg
    16501 cgtggcgact atgccgtgga tggcgaaaat cggcgtctta ctggcggggg ccgggttttc
    16561 gctggtgttc ccggcattgg gtgtagtggc ggtaaaagcg gttccgcagc aaaatcaggg
    16621 ggcggcgctg gcaacttaca ccgtatttat ggatttatcg cttggcgtga ctggaccact
    16681 ggctgggctg gtgatgagct gggcgggcgt accggtgatt tatctggcgg cggcgggact
    16741 ggtcgcaatc gcgttattac tgacgtggcg attaaaaaaa cggcctccgg aacacgtccc
    16801 tgaggccgcc tcatcatctt aaaacttact gaataaccag cgtattaatg atgttttctg
    16861 cggtggtctg cgctttttgc tgatcgtcag cgggcagcgt aatttgcatg gtcagcagtt
    16921 gattacccac gttacccaga ataacggaag agtacgccgt ctggcctttc gcggagataa
    16981 tactgtctaa ctgctgcatt ttgtgacctt tcagctcaat ggctttattg gttaccactt
    17041 gcagctgcgg atcgcggcta cgttgctgat cttccagacg cttcgccagc accgccagat
    17101 cttctttcgg atcatcgccc atgatgacga tgactgcttt ctgcccggtg gcgtcggacc
    17161 agacatgcat gttattggcc tgcgttccca gcttaccgct ctggtcggtc atatccgctg
    17221 gcagcgagaa acttaacttg ccatcaagca ggttgacggg attcccggta gcgttacttt
    17281 ccgcgaccga accctgcgcc gtagcgttag tgtctttatc atcacaggcc gcaagcccca
    17341 taaccagcag gccaattccg acatatttaa ccagattgcg cattgacttc ttcctttcga
    17401 taaacggcca taacggctca ttcatccatc ttatcacaac tctgataacg aacctttaac
    17461 tcgcctgcaa agcgttgatt tcggatttgt ctgccagtct tttcaacagc atattgagta
    17521 atacgccata cattggcagg aagaaaacga tactgattaa cactttgaaa cagtaatcga
    17581 ccagcgcgat ttccatccag tgttcagcca taaaggcatc cgggctacgc cagaaggcaa
    17641 tgaagaaaaa ggccagcgtg tcactgacgt taccgaacag tgtggacgct gtcggtgcca
    17701 gccaccagcg gcgactttga cgcaggcggt taaaaacgtg cacgtcgagg atttgcccca
    17761 gcgcgtaggc catgaaactg gcggtggcga tacgggcgac aaacaggttg aagtgggcga
    17821 gtgcgccgaa tccctgccat gaacccatat agaacagcga cgagatgaca taggagatta
    17881 ataacgcagg gatcattacc gcgaagataa tgcgtcgggc cagcggtgcg ccaaaaatac
    17941 gcacggtcag gtcggtcgcg agaaagataa acggaaaact aaacgcgccc caggtggtat
    18001 gaaaaccaaa aatggagacc ggaagctgga ccagatagtt actggaggtg atcaccagca
    18061 gatgaaataa cgataaccag aacaacgcct tatagcgttg agtttgcgag aaaacgttca
    18121 tattgtacct ttttgattaa ccattggggt gagggaaccc aatacgtacg acacgtctgt
    18181 tatcttaaca taataacgtg cttacccttt tttcgagccg ccgcatgata ctgctttacg
    18241 ttgacaatgc aatggttaat tttcacgcaa tcgttaacct ggtttgctta cggactcaca
    18301 gggcgtaaac tagcgccgtt tttttaagtg atgagaagaa aatgaccgat ctcttttcca
    18361 gccctgacca cacactcgac gcgcttggcc tgcgctgccc ggaaccggtg atgatggtgc
    18421 gcaaaaccgt gcgcaatatg cagcctggcg aaactttact gattatcgcc gacgatccgg
    18481 cgacgacccg cgatattcct gggttttgta cctttatgga acacgaactg gttgcgaaag
    18541 agacggatgg actgccttat cgttatttga ttcgtaaagg cggttgatgg ggaaagattg
    18601 gcttcgatgc cgccttttcc cctcacccta accctctccc cagaggagcg aggggaccga
    18661 tcgcgctcaa tgttgcgatc ggtttgcctt atctcctgcg caacaatctt aacgcattcg
    18721 ctgttaccag cacggtcgca ccggtatctg ccagcaccgc cagccatagt ccggtcatcc
    18781 ctagcagcgt ggtgacgagg aagatccctt tcagccccag cgcaatagtg atgttctggc
    18841 ggatattggc gtgagtggcg cgtgccagtt caatcatttg caccaggccg cgcaggtggt
    18901 tatgggttaa tgctgcgtcg gcggtttcca gcgccacgtc tgtgccactg cccattgcaa
    18961 tcccaatggc ggcagctttc atcgctggcg cgtcgttaat accatcaccg accatcgcca
    19021 ggggcgcatg ttgattcagc ttggtcaccg ctttgacttt atcttccggc aataggcccg
    19081 ctttaaactc cagccccagc tccccggcaa ttgccgccgc tgcgcgtgga ttatcgccgg
    19141 tgaggatcac tcctttaacc cccagcgcgt tcagttcact gatggcagtt gctgcgtcgg
    19201 cgcgcagggt atcctgcaat gcaatgatac ccagcacgtc atcattacgt actaccagca
    19261 caaccgtttg cccggcgctt tccagttcat taatcaaacc agcaaatgca tcagcgggat
    19321 gtttcccggc agcgcaaatc aatacgcgct caccgttaac ctgcgcttca atgccagacc
    19381 cgaccagcgc ccgctgtgat tcggcggtgg gaatggcgag ttcagcaacc tgtgcttcgc
    19441 gtacgatggc ctgcgccagt ggatgcgtcg cgccttgctc gaccgccgcc gccagtgtca
    19501 gcagttcaga ttcactaata cccgttgccg gatgaatcgc ggtaacgcgc ggtttaccga
    19561 cggtcagcgt accggtttta tcaaacgcca cctgagtaac acgacccagc tgttccagcg
    19621 ccgctccgcc tttaatcaac gccccacgac gcgctgccgc cgccagcccg gaggtaatcg
    19681 ccgcaggcgt ggagataact aacgcacacg ggcagccaat cagcagcagc gtcagccctt
    19741 tataaatcca ctcctgccag ctggcggcaa acagcagcgg tggcaccagc gtcaccagca
    19801 gggcgacggc cataatcgct ggcgtataaa tacggctgaa acggtcgata aaccgctcaa
    19861 tgggggcgcg gcgctcttcg gcttcttcga tcagtttcag aatccggtca atggcgctgg
    19921 cacccggttc tgacagcact tccagcgtta ccagacggtc tacgctggtg gctcctgcag
    19981 gaactttatc gcccgtcgcg cgctccaccg gaatggattc tccggtcagg gcgctttcat
    20041 caaaactggc aaacggtgag agcagtttac cgtcggcagg caaacgccca cctgcggcga
    20101 cttcaatcac atcgccaggc cgcaggctgt taatcgccac ctcttcccgc tcaccgttac
    20161 gcaggcgcgt ggcggtttct ggtttcagcg ccattaacgc gctaacgccc tgacgcgcgc
    20221 ggctggcggc ccagccttcc agtcgttcac caatcaaaaa cagcagcaac accatcgcag
    20281 cttcagccgt tgcgccaata aacagtgcac caatagcggc tacgctcatt aaggtttcaa
    20341 tggcgaagta gctgccggat ttgatcagcc gtaatgcctg acgagcaatc gggtacagcc
    20401 caaccagcgt ggtcgcgata aacgccagtt gcccaaacgg atgattaaat tgctccagac
    20461 cccagctgat tgccatcatc acgattagcg taatcagcgg cagattctct ttcaggcgcg
    20521 atgcttgtgg ctcgtcggtg gcctgctcat cgcgcaggga atagcccgct ttttgcaccg
    20581 cagattcaac ctgggcacgg atgtcattgt cggcatcgac caccagtttt tcggtggcga
    20641 acagtacctg cacctgattc acacctgcaa gctggcgcac ggcattttct accttgcgcg
    20701 cacaggcggc gcagtccatg ccgctgactt tccagctata gcgggtgccg gagacgtttt
    20761 cagagagcgt tggcgagctg gagcatgcgc cgtcgcagca acagtcgttg gcgttctgta
    20821 ccgtggttag cggtttgaac gcagcaaatt gaggggcttt cttgccgtga ttgtcaggtg
    20881 tcgacatggc atcctccggt taagtttttt ctcattaacc gaaggataca ctctggagtc
    20941 gactccagag tcaagtttta tcagagatac agtgagcgga cgatgaggaa gtgaccggca
    21001 aagtagcagg ccgcagcaat cgcgttatcc gcacggaagc gtcggcgata gtggctcccc
    21061 agccagacaa agttactgat aaacagcaac gaagcgccaa caaacgcaga gagcgccgga
    21121 gcggtcggac ggaagaacca cagttcacct gccagccaca ccatcaccag cgtcatgccg
    21181 ataaacgtgc agataggcca acgatactct tccagccgcg tccagataat cgccagtaac
    21241 agcgcaccca gaaccagcaa caccagtggc agaggccaga agaaagagag cgtcatctga
    21301 ctggcgaaat agatggtgta cagcaggtgc gagaggaaaa acgcgccgat ggcgtacatc
    21361 agacgttgac gtggcaacag ggttagcgca tcgcccagca gtgaggcgca cagccctgcc
    21421 agcaccagat agctaatggc gtcgaacatc ggcgcttgcc aggccagtaa cagcaggaga
    21481 agaagggtta acggtttaaa cacccagcgt tgccaggttg gcccacgata cgatgcatcc
    21541 acagatagcc atgcggaaag acagacagcg ataaacgacc aaagcatctt agttccttga
    21601 attcgttatt ttttcgtaag cggaatgcct acggtcttgc ttccagttta gtggcgaggg
    21661 tggacggatg acaacaccat aactggcagg gtatgcttat ttccgcattt tccgatgagg
    21721 gataaagatg agcaaaccac ctcttttctt tattgttatc atttgcttaa ttgtcgtcgc
    21781 cgcatcgttt cgttttatgc aacagcgacg ggaaaaagct gataatgata tggctccgct
    21841 ccagcaaaag ctggtggtgg tgagcaacaa gcgggaaaaa ccgattaacg atcgccgttc
    21901 gcgccagcag gaagtgactc cggcaggcac cagtatgcgc tatgaggcaa gcttcaaacc
    21961 gcaaagcgga ggaatggagc agacgtttag cctcgacgcc cagcagtacc acgccctgac
    22021 agtgggcgat aaaggtacgc tgagctataa aggaacgcgc tttgtcagct ttgtaggcga
    22081 acaataaggt tattttttaa ctttaaattt cttctgccag accagcagtt caaacacgcc
    22141 gaaaaggaaa atccgcacct tttcggcggt ggtcatctgc gggccatctt tcggtaaagt
    22201 ggatttcaat agcgccagtt gcataccatg catcagcacg gtaaaaatca gcgccacgtt
    22261 aacgaaaata ttcagcgggc gcgggaaggg atgcaccaga ttgaggatta aaaatcccca
    22321 aacgcagagc attaacaaac gaccaatatt aatcagcatc actttctcct tgtgcttcgc
    22381 gttgatacag ccgataagcc acctgacccg ccactttttc ccgatgtaat gaccagtttg
    22441 ccggaacagt gggcagaccg ttttcgactt cgctttcgac ataaatcagg gcttcgtcag
    22501 ccagccagcc gttatcttcc agtaaattta tcgtctcttc taacaagcca cggcggaacg
    22561 gtggatcgac aaacacgata ttatgcggtg tacctttttg cgccaggaat gacattgcgt
    22621 tgctgttcac cacgcgtgca ttgcctgctt ttagtgtcgc cagattctta attaactgct
    22681 gagaaaccgc gcgatccatc tcaatcaacg ttgcccccgc agcatagcgc gacaacgctt
    22741 ccagccccag cgcgccgctt ccggcgaagc aatccagaca ttgggcgtca acaatgaccg
    22801 gagccagcca gttaaacaac gtttcgcgta cgcggtcggt ggtggggcgc agacctgggc
    22861 tatcaggaac cgggagttta cggcctcgcc actgcccgcc aataatgcgg atttggccgc
    22921 tgccggaatg attcggtttt ttcatgataa attgctcaat ccgccagata acacatactt
    22981 gcaggcggtg atgtgaatta agttaagtga tagactattt catcattttt ttagctgcta
    23041 tgtacatagc gttaacgctg tgccatgaag caacagcgag gagtgtagtc gcaaatggcg
    23101 aaagaaaaaa aacgtggctt tttttcctgg ctgggctttg gtcaaaaaga gcagaccccg
    23161 gaaaaagaga cagaagttca gaatgaacaa ccggttgtag aagaaatcgt tcaggcgcaa
    23221 gagcctgtga aggcttcgga acaagccgtt gaagagcagc cgcaggcgca tactgaagcc
    23281 gaggcggaaa cttttgctgc cgacgttgtg gaagtcactg aacaggttgc tgaaagtgaa
    23341 aaagcgcagc ctgaagcgga agtcgttgca cagccggaac cggtcgcaga agaaacgccg
    23401 gagccagtgg ctatcgaacg tgaagagctg ccgttgccag aagacgtcaa cgccgaagag
    23461 gtttcgccag aagagtggca ggctgaagcg gaaaccgtag agattgtcga agcggcagaa
    23521 gaagaagcgg ctaaagaaga aatcaccgac gaagagctgg aagcacaggc gctggctgcc
    23581 gaagcggcag aagaggcggt gatggtagtt cctccggcag aagaagagca gccggttgaa
    23641 gaaattgccc aggagcaaga aaaaccgacc aaagaaggtt tcttcgcgcg cctgaaacgc
    23701 agcctgttaa aaaccaaaga aaatctcggt tccggattta tcagcctgtt ccgcggtaaa
    23761 aaaatcgacg atgatctgtt tgaggagcta gaagaacagt tgttgatcgc cgatgtgggc
    23821 gtggaaacca cacgtaaaat tatcaccaat ctgacggaag gcgcatcccg caagcagctt
    23881 cgtgatgccg aggcgctcta tggcctgctg aaagaagaga tgggcgagat tctggcgaaa
    23941 gtcgatgagc cgctgaatgt tgaaggtaaa acgccgttcg tgatcctgat ggtgggcgtc
    24001 aacggtgtgg gtaaaactac gacgattggt aagctggcac gtcagtttga gcagcagggt
    24061 aaatcggtga tgctggcggc gggcgatact ttccgtgcag cagcggttga acagcttcag
    24121 gtctggggtc agcgcaacaa tattccggtg attgcccagc ataccggtgc ggattccgcc
    24181 tctgttatct tcgacgccat tcaggcagct aaagcgcgta atatcgacgt cctgattgcc
    24241 gataccgccg gacgcctgca gaacaaatcg cacctgatgg aagagttgaa gaaaatcgtc
    24301 cgcgtgatga agaaactcga cgttgaagcg ccgcatgaag ttatgctgac tatcgatgcc
    24361 agtaccgggc agaacgcggt aagccaggcc aaactgttcc atgaagctgt tggcttaacc
    24421 ggcatcacgc taacgaaact ggacggcacg gcgaaaggcg gggtaatttt ctcggtggct
    24481 gaccagtttg gtatccctat ccgctacatt ggtgtcggcg aacgtattga ggatttgcgt
    24541 ccgtttaagg cggacgactt tatagaggca ctttttgccc gagaggatta acaatgattc
    24601 gctttgaaca tgtcagcaag gcttatctcg gtgggagaca ggcgctgcag ggcgttacgt
    24661 tccatatgca gccgggtgag atggcgtttc tgaccggtca ttccggcgca gggaaaagta
    24721 ccctcctgaa gctgatctgt gggattgagc ggcccagcgc cgggaaaatc tggtttagcg
    24781 gccatgacat cacgcgtctg aaaaaccgtg aagttccgtt tctgcgccgc cagattggca
    24841 tgattttcca ggatcaccat ctactgatgg accgtactgt ctacgataac gtggcgatcc
    24901 cgctgattat cgctggtgcc agcggtgacg atattcgtcg ccgggtgtcg gcggcgctgg
    24961 ataaagtcgg gctactggac aaagcgaaga acttccctat tcagctttcc ggcggtgaac
    25021 aacagcgtgt tggcattgcc cgcgcggtgg tgaacaagcc cgcggtactg ctggcggacg
    25081 aaccgactgg taacctggac gacgcgttgt cggaagggat tctacgtctg tttgaagagt
    25141 ttaaccgcgt tggggtaacc gtattgatgg caacgcacga catcaacctg atttcgcggc
    25201 gttcctatcg catgctcacc ctgagcgatg gtcacttgca tggaggcgtg ggccatgaat
    25261 aagcgcgatg caatcaatca tattcggcag tttggcgggc gtctggatcg cttccgtaaa
    25321 tcggtcggcg gctcaggcga cggcggtcgt aacgcaccga aacgcgcgaa atcctcgcca
    25381 aaaccggtaa atcgcaaaac caacgttttc aacgaacagg tgcgctatgc cttccacggc
    25441 gcattgcagg atctgaaaag caaaccgttc gccacgtttt taacggtgat ggttatcgcc
    25501 atctccctga cgctgcccag cgtctgttat atggtgtaca aaaacgttaa tcaggcggcg
    25561 acgcagtatt atccgtcacc gcaaatcact gtttacttgc aaaaaacgct ggacgatgac
    25621 gccgctgcgg gcgtggtggc acagttgcag gccgagcaag gcgtggagaa agtgaactat
    25681 ctttctcgtg aagacgcact gggcgagttc cgtaactggt ctggttttgg tggtgcgctg
    25741 gatatgctgg aagaaaaccc gcttccggca gtggcggtgg tgatcccgaa actcgatttt
    25801 caggggacgg aatcactcaa tacgctgcgt gatcgtatca cccagattaa cggcattgac
    25861 gaagtgcgga tggatgacag ctggtttgcc cgtctggcgg cgttgaccgg gctggtcggg
    25921 cgcgtttccg cgatgatcgg cgtgttgatg gtggcagcag tgttcctcgt catcggcaac
    25981 agtgtgcgtc tgagcatctt tgctcgccgt gactccatta acgtgcagaa actgattggt
    26041 gcgacggatg gtttcatcct gcgcccgttc ctgtacggag gcgcattgct gggattttct
    26101 ggcgcattgc tgtcattaat tttgtcagaa attctggtac tgcgtttgtc atcggcggtt
    26161 gcagaagtgg cacaggtttt cggaacgaag tttgatatca atggtttatc gttcgatgaa
    26221 tgcctgctat tgctgctggt atgctcgatg attggctggg tggcagcgtg gcttgccacg
    26281 gtacaacatt tacgccactt tacgcctgaa taataaaagc gtgttatact ctttccctgc
    26341 aatgggttcc gtagcaggga aagagatccc gttgtctctt cccggtattt catctctatg
    26401 tcacattttg tgcgtaattt attcacaagc ttgcattgaa cttgtggata aaatcacggt
    26461 ctgataaaac agtgaatgat aacctcgttg ctcttaagct ctggcacagt tgttgctacc
    26521 actgaagcgc cagaagatat cgattgagag gatttgaatg actgacaaaa tgcaaagttt
    26581 agctttagcc ccagttggca acctggattc ctacatccgg gcagctaacg cgtggccgat
    26641 gttgtcggct gacgaggagc gggcgctggc tgaaaagctg cattaccatg gcgatctgga
    26701 agcagctaaa acgctgatcc tgtctcacct gcggtttgtt gttcatattg ctcgtaatta
    26761 tgcgggctat ggcctgccac aggcggattt gattcaggaa ggtaacatcg gcctgatgaa
    26821 agcagtgcgc cgtttcaacc cggaagtggg tgtgcgcctg gtctccttcg ccgttcactg
    26881 gatcaaagca gagatccacg aatacgttct gcgtaactgg cgtatcgtca aagttgcgac
    26941 caccaaagcg cagcgcaaac tgttcttcaa cctgcgtaaa actaagcagc gtctgggctg
    27001 gtttaaccag gatgaagtcg aaatggtggc ccgtgaactg ggcgtaacca gcaaagacgt
    27061 tcgtgagatg gaatcacgta tggcggcaca ggacatgacc tttgacctgt cttccgacga
    27121 cgattccgac agccagccga tggcaccggt gctctatctg caggataaat catctaactt
    27181 tgccgacggc atcgaagatg ataactggga agagcaggcg gcaaaccgtc tgaccgacgc
    27241 gatgcaaggt ctggacgaac gcagccagga catcatccgc gcgcgctggc tggacgaaga
    27301 caacaagtcc acgttgcagg aactggctga ccgttacggt gtttccgctg aacgtgtgcg
    27361 ccaactggaa aagaacgcga tgaaaaaact tcgcgccgct attgaagcgt aatttccgct
    27421 attaagcaga gaaccctgga tgagagtccg gggtttttgt tttttgggcc tctacaataa
    27481 tcaattcccc ctccggcaaa acgtcaatcc ccacgcagat tgttaataaa ctgtcaaaat
    27541 agctattcca atatcataaa aatggggtat gttttagcag agtatgctgc taaagcacgg
    27601 gtagtcatgc ataaaacgaa ataaagtgct gaatcacaac accacaacac acgtaataac
    27661 cagaagaatg gggattctca ggatgaacat aaagggtaaa gcgttactgg caggatgtat
    27721 cgcgctggca ttcagcaata tggctctggc agaagatatt aaagtcgcgg tcgtgggcgc
    27781 aatgtccggt ccggttgcgc agtacggtga ccaggagttt accggcgcag agcaggcggt
    27841 tgcggatatc aacgctaaag gcggcattaa aggcaacaaa ctgcaaatcg taaaatatga
    27901 cgatgcctgt gacccgaaac aggcggttgc ggtggcgaac aaagtcgtta acgacggcat
    27961 taaatatgtg attggtcacc tctgttcttc atcaacgcag cctgcgtctg acatctacga
    28021 agacgaaggc attttaatga tcaccccagc ggcaaccgcg ccggagctga ccgcccgtgg
    28081 ctatcagctg atcctgcgca ccaccggcct ggactccgac caggggccga cggcggcgaa
    28141 atatattctt gagaaagtga aaccgcagcg tattgctatc gttcacgaca aacagcaata
    28201 cggcgaaggt ctggcgcgag cggtgcagga cggcctgaag aaaggcaatg ctaacgtggt
    28261 gttctttgat ggtatcaccg ccggggaaaa agatttctca acgctggtgg cccgtctgaa
    28321 aaaagagaat atcgacttcg tttactacgg cggttatcac ccggaaatgg ggcaaatcct
    28381 gcgtcaggca cgcgcggcag ggctgaaaac tcagtttatg gggccggaag gtgtggctaa
    28441 cgtttcgctg tctaacattg cgggcgaatc agcggaaggg ctgctggtga ccaagccgaa
    28501 gaactacgat caggttccgg cgaacaaacc cattgttgac gccatcaaag cgaaaaaaca
    28561 ggacccaagc ggcgcattcg tttggaccac ctacgccgcg ctgcaatctt tgcaggcggg
    28621 cctgaatcag tctgacgatc cggctgagat cgccaaatac ctgaaagcga actccgtgga
    28681 taccgtgatg ggtccgctga cgtgggatga gaaaggtgat ctgaaaggct ttgagttcgg
    28741 cgtatttgac tggcacgcca acggcacggc gaccgatgcg aagtaatcat taatcggcaa
    28801 ctttgggttg ccgccaaatt gctaatatcg agtacgttgc ttcatgccgg atgcggcgta
    28861 aacgccttat ccggcctaca agatccaaag aaatcagtaa attgcaacac acattgtagg
    28921 cctgataagc gtagcgcatc aggcaatttc tcgttggtca tcacttttga aatcaacttg
    28981 acgattaacg cttctcccag ccgccctgtt gcgccgtaaa ccccagcgcc tgcataaacg
    29041 ccgtcatcac accgcgatct tccacgcctg catccgccat ccaccagcat gaaacggcag
    29101 gattgttacg caaaacctct tccagcagat attgccccac accgcgacgg cgggtgactt
    29161 cccgcacgcg cagggaatcc agtgctccct cggtgccgct taaggttacc cgcacggccg
    29221 ctagcaggcg ctcgttaaaa cgcgcggcgt agatgcggtg gttatcgtca acctgtaaag
    29281 aggaagggga atactccggc cagatctttt gcaggtcaat ccggtcctgg tcgctaaagt
    29341 tttctaatcg aatgatggtc agcttcatgg ggaacccgtg taaatcacaa aagtggaacc
    29401 agtgtagcga aat
//
LOCUS       DAZGVQ010000054        29094 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000054 DAZGVQ010000000
VERSION     DAZGVQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29094
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.054"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(208..729)
                     /locus_tag="ACHLAG_002240"
     CDS             complement(208..729)
                     /locus_tag="ACHLAG_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0655464.1"
                     /translation="MRTLDITYGIPAEVWPRDYEHAERTLLFWRSAKFPVRVTLEDGQ
                     VFCMYVYGLLSARNKLNLTPDPLSNTNRIRLPLERISTIESGIEEVDTSFTGRLTLNN
                     EQMVNQPSRRDFFKICRQAYQNGKSIRVYMADGREIEGTSDGVDACLVTLKLEDGRKI
                     LIFFDWVERILPF"
     gene            complement(726..1319)
                     /locus_tag="ACHLAG_002241"
     CDS             complement(726..1319)
                     /locus_tag="ACHLAG_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655465.1"
                     /translation="MKRSLLLACIYLSSFSANALSDIVSDMQKTYSMYTSEDVLKYES
                     RGLIQPVWFRFSDNAEETILNAGKERLRLQINGTAVPENVNVSSDEAMKPSGIVLEVQ
                     DGTLYCDEMATTGCQVNIRVGNEEPIVTYAKAEGNTSGKVLFIDENISKRIVRDIHKT
                     ANSKSVDKKIYVEVPFFVVGNKEFKFGLLKTPLRVSE"
     gene            complement(1500..1778)
                     /locus_tag="ACHLAG_002242"
     CDS             complement(1500..1778)
                     /locus_tag="ACHLAG_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655466.1"
                     /translation="MSEKKTTYCQVALSDKANDKLGKFQMKLKEKNIKMSKAEVINTI
                     LEAMTMAEFDKVSSAVGVSAKAREKIMRIYENSNMTKEDLEQILSKLP"
     gene            complement(1781..3340)
                     /locus_tag="ACHLAG_002243"
     CDS             complement(1781..3340)
                     /locus_tag="ACHLAG_002243"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP0655467.1"
                     /translation="MTVRIVSNAVNALISGADDKVKQLVQQMLSYEVETGDWKGTSTM
                     FNWSKNSFPAGFAKPVAANLNKAGIKCVHIRKDKAPALGKPNPAVNPFPYNPDYAYQD
                     QTVETLVREGMMIAQIATGGGKSNVACKAAARIGRMTLFLTTRSVLMFQMAENFQRSI
                     DYRAENGEPWLKDQKVGVIGSGEFQVSRHINVATVQTLASFLEEPPRDATPDKKSYHL
                     KRRELVKRFLSSVSLLILEEAHESSGSNFYDIARLCVNADYRLALTATPFMKDSTEAN
                     MRLMAVAGRIEIKVTEKYLIDRGILAKPYFLYHKVAYKPDEARIKAELANKHLNFRVG
                     MSTAYQKAYQLGIVYNLGRNEAIVREALLYKQHSLNCMTLVRLKRHGQILMEMMKESG
                     LRVDFIYGESNQATRQAKLNSLASGEIDVLIGSTILDVGVDVPSVGAVILAGGGKAEV
                     EMRQRVGRGLRAKKNQANVCFITDFIDISNKYLLSHSYERKHIIDTTPGFAEGVLPID
                     GAFDFGVLKRD"
     gene            3423..4103
                     /locus_tag="ACHLAG_002244"
     CDS             3423..4103
                     /locus_tag="ACHLAG_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP0655468.1"
                     /translation="MDVKITILQVDVANLRPNTWNTNSVGAQNFEKLKGSIEKLGFFK
                     PILARELEDGFFEILGGEHRWRAAIEQGISTVPVLSVGKISDVVAKQMSLVDNERYGE
                     DDQIALQRFIEEIQSELDYQLSEIAPYDDEISMVLAKEAAIDLEALEALSRGSDEPVD
                     TDKREKTERVGAEHQTMRFKVTFDASDRVAETIKNIIKEQAINTGNEMENAGEALVWL
                     VDYYKERM"
     gene            4103..4771
                     /locus_tag="ACHLAG_002245"
     CDS             4103..4771
                     /locus_tag="ACHLAG_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/Srx family N-terminal domain-containing
                     protein"
                     /protein_id="HFP0655469.1"
                     /translation="MTKKFEIVYRDPADLIPYEMNAKKHDEQQIRDLAAAIKKRGFDQ
                     PITVDKNDVIITGHGRREAAIFAGLERVPVIVRDDLSDDEVRAKRLEDNRLASIDYDA
                     IKLQKELESLVLDDIEVFGFEERELNVLVGSMTEEMDTDSLVIDLGEETKRQKDEHTE
                     ISREVAAEEVRVVDVLGFKTLPAGSAIVVGDLLAHMEEMTGESGVDAFVAYAEKISSG
                     EMAA"
     gene            4768..5433
                     /locus_tag="ACHLAG_002246"
     CDS             4768..5433
                     /locus_tag="ACHLAG_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP0655470.1"
                     /translation="MSKYIINVSFQTRVNKTTRTLEIAESFGLGLDEKEWTLYDNLEL
                     EVKQGDVVYITGQSGSGKSVVLRELQRQMKDEGLSVASIDDFTFDNEVNVIDQLGKTT
                     SDALGLLSMAGLNDAYLFVRKPSEMSDGQKYRLKIAKLIESGAKVWAADEFGAVLDRV
                     TAQVVASNLQRAARKVGATVMVATTHEDLKNALRPDMQITKHYKERVKVEYHNGSHDE
                     VHL"
     gene            5430..6320
                     /locus_tag="ACHLAG_002247"
     CDS             5430..6320
                     /locus_tag="ACHLAG_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655471.1"
                     /translation="MTDIIIKRYRPEEYPRHLDFLERMTVTKGTVEDWYALKSLHYKT
                     DGKPFAPTYYRCELDDRLVGVVVMAYPKLLLAPRHRMFPKLKPTTNTTVANQYWGRYV
                     NNNFAVISRSVVDTQYRGVGVSYRMINLVSRMHDRPIIEIQSSMSKYNPFAMKAGFKF
                     IRPERPKSYESALRVFQRHFRSDPGDNEAIVKELFAMSESRRRRALRDLVADYHKNSS
                     LAKAGRNRGTTIQDIADSLVDEASIVKLLKDIHNLSFTSPLYGVYRNPDFGRRLPDTL
                     PLLAFDKQPLDKPLEIALPA"
     gene            6330..6596
                     /locus_tag="ACHLAG_002248"
     CDS             6330..6596
                     /locus_tag="ACHLAG_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655472.1"
                     /translation="MTLTDKQKDIIKTLNLGYERGHLLDLDELLEVLPYKTTKQSIQF
                     SIRALIKKGLVEKGHTRQRSDNRYHRRTLGLTTLGRAKAKLLVM"
     gene            6793..7425
                     /locus_tag="ACHLAG_002249"
     CDS             6793..7425
                     /locus_tag="ACHLAG_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0655473.1"
                     /translation="MTVEKDEVKTRLTPAEWAEAEAKWTSGEYTLSKLEEEYGIRRET
                     LSRHFKKRGLEKGADSVGKMVRESLKSDAELRAKARAEKIEERRTRYDGWAYALGQMV
                     MVEVTTAKREGKPLGAIEDSLKSLQRASNTLAKCFEVSSKALGMDHAENDEEEIPNLV
                     FGELTPSQVAKLRQEDDEPEIIDDELLETLEEEALSEFDATDDGIEGEDE"
     gene            7425..8681
                     /locus_tag="ACHLAG_002250"
     CDS             7425..8681
                     /locus_tag="ACHLAG_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFP0655474.1"
                     /translation="MAIPSSLSLVQLHSGQMKVFQSPHRFKVVCAGRRWGKSRLSIST
                     IIRAAAKEKKQRVWYVAPTYQMARQILWDDLQEVLPRQWVRKKNDTTMTIVLKNGSEI
                     ALKGADKPDTLRGVALHFVVLDEFQDMKADTWYKVLRPTLSSTRGGALIIGTPKGFSE
                     FHKLWTIGQNVELQRKGQWKSWQFVTADSPFVPTAEIEAAKNDMDPKSFAQEYLASFE
                     NMSGRVYYPFDRNVHVKPLQFNPRLPIWVGQDFNIDPMSSVILQPQPNGELWAIDELV
                     LFSSNTAEVCDELERRFWRWKSQITVFPDPAGAYRQHARGESDVDIFKEKGFLRVDYS
                     KKHPPIADRVNAVNRMLMTASGDIRLYIDPKCKHLIDSLEKVIYKPGTRDMDKTGGIE
                     HSADALGYPVHRRYPVKNRVILGGSR"
     gene            8708..10282
                     /locus_tag="ACHLAG_002251"
     CDS             8708..10282
                     /locus_tag="ACHLAG_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0655475.1"
                     /translation="MELNDKQIKDLVARRHPEYEKKKEHWDFLASTYAGGRGWFTDNI
                     FRYFKEGDQEFKERVERAYRFNHTREVVNLINKYLFKEDIHRNIEEAPEQIRNFWKRA
                     TRQNASIDSFMAAIDLQSSIYGRIWVVVDSTMSGDVESVADEKKKDARAYAYWISPQQ
                     MLDVAWDDDGNMLWALIVEVARDDADPFTSTGQEYQRYRLWTQNEWYLFREEVKKGAG
                     GAGRRQAKVILEDSGEHNLGVVPVFPVDCIGESESPYFSPSLIDDIAYLDRAVANYLS
                     NLDAIIQDQTFSQLAIPVQSLLPGDENHTKVLEMGTKRVFTYDSEGGNQPFYLSPDPK
                     QAQMIITTIKTVINEIYHSVGVAGERTKQDNAQGIDNSSGAAKMYDFQRVNSLLVTKA
                     ERLERAERQIMLLVAKWMGVDLDEDHSLIAYPESFDIRGLTDEFSVAEKLSLLQAPDS
                     VRRHQMEMLIEKIFPNITEAMKKEFDKDLLNFPPKNDLNTLENKSVLTYDQGAAQESG
                     QDQPRGNGDSSTQENE"
     gene            10304..11191
                     /locus_tag="ACHLAG_002252"
     CDS             10304..11191
                     /locus_tag="ACHLAG_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6651 domain-containing protein"
                     /protein_id="HFP0655476.1"
                     /translation="MQLWQMLLARRGLMGVAEAHERGGAGGVAADNEQSTQDPDKQGE
                     QKEQPKGDDEYAGMTHEELLAELRKTKKAGAELLKENMKRKEKERTLADQLAQYGDID
                     PARARQLLEAEQAAENARREAEQAELERRGEFDAVKKQMIEAHQAELAQRDERYTALE
                     SENASLKSQLVEMTVGASFSNSLFLRDKVLMTPAKARVIYGSHFEVGEDGSVVGYDKP
                     AGHKERAVLVDGEGKPLPFESAIERIVRADPEADALMRSEAKQGVGSHSKSTYTISQP
                     KNKSTMDKLASGLGKIGLK"
     gene            11217..12095
                     /locus_tag="ACHLAG_002253"
     CDS             11217..12095
                     /locus_tag="ACHLAG_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP0655477.1"
                     /translation="MPLLREEAEKLSNNELEQGVIETIIDRDDLFAILPFMKINSKAY
                     LYNREKTLSEATFIDVNDTITEGAATFEEKVAKLRILAGDVDVDKFLATTMADTNNQL
                     AIQVRQKVKGLARAFRRNLIVGDSTTNNKAFDGIPKLMHDDQKIDISGASMTFSMFDE
                     LVDAVKDLGADCIMMRSEHLRAYRALLRTVNVGPSEIMMENFGRPMLCHNGVPFIVND
                     FIPVVDSTKADIYCLHLSEENGVTGLYGGDNAGIVVENIGTVQNKDAVRTRVKWYCSL
                     ANKHDKAIAALTNVKI"
     gene            12169..13089
                     /locus_tag="ACHLAG_002254"
     CDS             12169..13089
                     /locus_tag="ACHLAG_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HFP0655478.1"
                     /translation="MPEQKMKITEEAFSDFTGHMCRAGFTNSISDEPLTERQQSQLSA
                     CLQAVPFSQSVNITPASPSVLVGKTVQLSAGISMGKSASSFTWKSANDQIATVNGTGL
                     VTGVAPGKVKITATDQETQLSASVEVTVNPVAVQSVTVTPESTSVEKGKSVSLKANVQ
                     PSNATNKAVTWSSKNEDKATVDQSGNVTGVEVGTATIEIVSQDGSKKATATVEVTAPV
                     VAVTGVEIDPNSTTVEANKTVQLTANVEPAGATNKTVTWESKNTEFATVDSETGVVTG
                     VAAGTATIEVTTQDGSHKATATVEVTVAQE"
     gene            13134..13568
                     /locus_tag="ACHLAG_002255"
     CDS             13134..13568
                     /locus_tag="ACHLAG_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655479.1"
                     /translation="MKPAKIVLLEPQFSGYSGMLCGVQFENGVSVAELPFIDQQRICA
                     SMRASTVEGKNVSPSAAYSDRGELTADLITEPAAPDIVPMKRGTPDEPAKQIQTFTRE
                     ELESIADNEGIAGLRVIGNQVGVKAKGIVEMIEGILKAQGGE"
     gene            13568..14401
                     /locus_tag="ACHLAG_002256"
     CDS             13568..14401
                     /locus_tag="ACHLAG_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655480.1"
                     /translation="MAQIDSYRSGEAVSLSFAFNVLDIESATYTVKDSTGAILVDGEP
                     LEVTSGQMSIPVVVSAEYNQLSEKERDLRYVIVKAVASGLTHEERQMYVLLNSFELSI
                     PEQSFATVADAQMQAIDMLNGDTLLSDGEGLMRKRLIEATRRIKTLPFSIRKILRIDF
                     DRYDRPQNMLNVYDIPWGADGAYRHDLVDWEKMTQEKFEEFPDYFKEALMLAVVNEAC
                     EIANGNDVAAAREDGILSESIGETTNMYRTGKAANVHVARSTWRLLVSYINNRMIVRR
                     A"
     gene            14481..14825
                     /locus_tag="ACHLAG_002257"
     CDS             14481..14825
                     /locus_tag="ACHLAG_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655481.1"
                     /translation="MNISWQTEIAIYRFGAKNVYGEAQLQFVRKTNVGVVKFEQSNEK
                     SSVRADSSGSRGKASLELFDAVLVVPLEAAVQLDDVLILEGQKLKVSSVHRRWGLRGR
                     PGHLEVGANIWV"
     gene            14816..15289
                     /locus_tag="ACHLAG_002258"
     CDS             14816..15289
                     /locus_tag="ACHLAG_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655482.1"
                     /translation="MGLKYDAHQFKRAGNRLNNSQKAFKRYLIRDMEKLARLVERLSR
                     AMAPLETGSLETAIFARVIKEGYSGLRIELSVSGAKPRQGHPGVEVGDYAKYMELGKY
                     RLGYLSRMKNVTNPPIAGVKPRVGPHFLERAVEISEKQFSEAILEAARKAGFTRG"
     gene            15291..15674
                     /locus_tag="ACHLAG_002259"
     CDS             15291..15674
                     /locus_tag="ACHLAG_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HFP0655483.1"
                     /translation="MFIEAFASLMQKAKIGTVGTDIFCHYLPANVKSGVLLINPNTGI
                     SIDHELQGFYHESFTIIVRGSSITTTVEKANKIIEMFPVEETESGGVYFRLVRPMAMP
                     IIYPKNDGALIEAGIPVEFAGYLLN"
     gene            15749..16495
                     /locus_tag="ACHLAG_002260"
     CDS             15749..16495
                     /locus_tag="ACHLAG_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001718041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP0655484.1"
                     /translation="MSNTHVKNIKLGACKVSFGGVDLGYTKGGVQVEVATETLKVTVD
                     QLGQTVISELVQGRNITITAPLAESVLQNMVDLMPGSTLSDDDSSVTITSAQGVNLID
                     VAKELVLTPQDTTDYVLTIPKAATAGNFTMTYQSDDVRVFSVQFSAYPDDEGVLGKMS
                     GPKPVKTVSISPESPTVKAGETVQLTAEITPADAADKTGVWESEDQEKATVDQTGLVR
                     GVAQGSTNISFTSNSGGKKATKSVTINSAG"
     gene            16551..16868
                     /locus_tag="ACHLAG_002261"
     CDS             16551..16868
                     /locus_tag="ACHLAG_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655485.1"
                     /translation="MTKLLDLDSILPPKKSIKFGGQEYPIVEMTVGLFVSIKQMEGKD
                     LMNMSPVEQVTAYADLVRKVIPSVPDEVLEKLTVPQLQQIFTFAMEVIDEENEKAAGE
                     GAK"
     gene            16994..17218
                     /locus_tag="ACHLAG_002262"
     CDS             16994..17218
                     /locus_tag="ACHLAG_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655486.1"
                     /translation="MLSRNIDRLRAEEDVRNLQVARASQADGEAVKAFMEGLQLRIGR
                     PVVTDKVYDPSQDKADPDAKEQLMQIFGRG"
     gene            17226..21797
                     /locus_tag="ACHLAG_002263"
     CDS             17226..21797
                     /locus_tag="ACHLAG_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFP0655487.1"
                     /translation="MSQNVEFILSLEDKQFTASIDRAGKLLTRFGEQVTKPAQKIQTF
                     ERSLGSVARIIGVLESKLDSTADKLQDVAAGFELASDATRKMRGNITSLNSGLKALIE
                     RVDTTTSSVDKLTASLRKVQSELNDFSDWATYASKSASRFGTEVKEASVSVSGMNTRL
                     NTTTKRLSNWGVTTSQAAEGLKKVREQMDEVIGRQQLISKPVRVRTSGSGDGGSGRRS
                     GASGHSGKSNEGGMFSGLRGNIFLLGEIGDAARTVTDIMFGWQKPIVEAAAEMERMRV
                     MLRGLNKEKSNPGQAAADDMKYIVDMAQNAPFAMQALTDSFVKFRSAGLDPTDGSLKA
                     LVDSVARFGGDSELLKRAAVAVQQMSGKGVVSMEELRQQLGEAVPNAMQAMADAAGIT
                     MGELTKAVASGTVEAKQALSLMFVGLRAENENAAKDMMQTYTGALAQLQTSFTLFADR
                     VGQAGYLDSLSKGMKELASIMNSAEGISFANSLGSGLTTAIDGLRQLAQWLAKNQELV
                     INLGKVVAAMVAFKLMRAGIMGVVGAGSQMVSTFATMATAIQTPFNLGATAVTRFNRA
                     ARMGLAPIPSLIFAIRGAITGLKGAFAGLTAFIAANPIGAAFTVATVAVAGLITYMTM
                     LRSETSKVVDEIRKIPEAMTAAKRAQMADRAEKLEKQILADQRALKTGEGVNYVSNSA
                     GVITHKESKADIEARLKKNQEEYQKITGTIALGDGAVAKRLAKEAAESQIEKIRAENQ
                     IFAATFVKARQEALDKIQKINDDGSLSDDEKNKLLAPLRETVNKSYLEPAQKLVDSLS
                     SRKNATEKQIATLNDQLEKAKKDGNTEQIQKLQGSIRGYQEHLEAVAQELTQAEFERD
                     NAAKTGKGVMSNQGTVLGLGTTDKAAQKALAQYMRNQMDSATYQRTLPDGTPMMDFEG
                     KPIIGPKQLKTQLNLQKASSASSLEKMSDEERAAAIAALTKAREQDAAAAEKAAQRSA
                     NASQRAAKKEQAAQQKLAAGYQKALDKADQLMGQMGESSKATVSFDQSLRDTTKSLTD
                     LANAVPNEFITQEMIDKAKKRLEDLKNATPEYREMFNRRNVEQMISTWAPESDSIISA
                     GYTPSREEKVADFEDTYNRNLKALIELRDKASDPKVVALYTKKINQLIAAGNTALIKE
                     TGTATQKLALEYENLAEQIESTWTDLFSGLTDVLTDFVINGKMSFSSLSQSILKDITN
                     MVVKSQITLPLMNMLGMGTTAAGSSQSGNLLTGVASAVANQGVRMGNTVNGDKSVGEA
                     TKETSSSVTGLGQTTQQTTSAIGTATNAIGSWVSGLFDSTEAKDAETKAVKDSIFSMQ
                     NLSSVTGALSAAFAMLGANASGSGNKWLNFGATVASGLVSVWAGGGFDGLMSGSSGST
                     SGFNNLTSSAADGTNGIPAIPKFANGGIFGKDGVIPLRAYQKGGIANSPQLALFGEGS
                     MNEAYVPLPDGRTIPVTLSTDGMSGGGNVLSPVSIEINVHSDGSTTESGDTESIWNNA
                     AQRMKAIALETIAQEKRPGGSLNPNTQRN"
     gene            21840..22175
                     /locus_tag="ACHLAG_002264"
     CDS             21840..22175
                     /locus_tag="ACHLAG_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0655488.1"
                     /translation="MERQTFNWYPDYESEKSVKPNVTVLNFGDDYEQRQAQGLNRIKE
                     EWSLTFTRSYNEINAIDDFLTERSGVESFYWVNPRGKQIVVVCDSHTVKRYQGYCVLT
                     ATFRQVFEA"
     gene            22258..22956
                     /locus_tag="ACHLAG_002265"
     CDS             22258..22956
                     /locus_tag="ACHLAG_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0655489.1"
                     /translation="MGIKADIQSLSPSALIELFVLDMSNTTSGGKLFFHAGTNELMQP
                     VVWQGVTYEPWPIKASGFDKTGQGTLPRPKIQVSNFAGTVSAEVQANDDLVGCRIIRK
                     MTLARFLDAVNFKDGNPTADPNQHFPDEMWFIEQKTLETHQVVEFELSSVFDLMGVQL
                     PYRQIIKNTCPWKYRGPECGYTGPYFDKNNQQTSMSGADYCTKRYDACNARRNYFADG
                     VIHFGGFIGATRYG"
     gene            22949..23746
                     /locus_tag="ACHLAG_002266"
     CDS             22949..23746
                     /locus_tag="ACHLAG_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0655490.1"
                     /translation="MGNKAIPELGSDVMQQIYLCAINRYPNEACGFLVRTNGDKYRFM
                     EARNVSENPQNTFVMHVDDIMAAEDAGDVIAIWHSHTDESAEASDADRAGCEATEVPW
                     MILAIRKNVEGDAPFHFSEMNVITPDGFEMPYLGRPYVFGVFDCWMLCRDYLKREFNV
                     ELNPNAHLHIPSWYTGDNDILDQNYRNEGLVRLAPGTEPQRGDVFFIQYGKMPDHCAV
                     YIGDGMILHHQIDRLSCRAYYGGMYQKHTTHHLRHRDLLKGDETCLS"
     gene            23734..24327
                     /locus_tag="ACHLAG_002267"
     CDS             23734..24327
                     /locus_tag="ACHLAG_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0655491.1"
                     /translation="MSELVHVQLGGPMAKHFGRHWHLKVRNTKQALDLIEANKPGFKA
                     WMKRNIKTYDRYHIQITNKQGHKWSVDESEYQMMGQSDNIAKIRITPVPRGSGGSAFG
                     WFQTVVGAALLVVSAVVMPALAPLGLSLMMGGISQIISPQATNESVRQADNSNSYYFD
                     GPQNTENQGNPVQLIYGEEILVGSQVVSSSITIDQLM"
     gene            24345..29072
                     /locus_tag="ACHLAG_002268"
     CDS             24345..29072
                     /locus_tag="ACHLAG_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003993213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0655492.1"
                     /translation="MEQFKKKKLPLFIAGAGGKKSSKSSSRTPVEADDTVNSRAMAAI
                     LDLLGEGVIGGLVNGARSIFIDDLPIVNEDGSSNFSGITWDFRDGSQDQTPMSGFDFV
                     ETPKSVNIQLKKTHYVTVSIDNDEADRVRVIMKFPSLRSIDKKTGDTNGTTVEYKFQI
                     ANGDSTFVDVVAEGEKSVGIKLTAKKTGVYYRSYELKLPKPGRAYKVRVVRITDDNSS
                     QYLYNDTWVDSIGEIVDTPMNYPNSVLVGLKVNSEQFGSTMPSRSYLVRGLKIRVPSN
                     YNESSNTYAGVWDGTFKLLSSSNPAWILFDLLTNARYGLGQYVSESMIDLGQLYQIGR
                     YCDEEVDDGFGGKEKRFAINTQITSRQDAYRLIQDIAGAFRGMVFWAGGMVNIMQDSP
                     SDPVMMFTNSNVKDGLFTYKGSARKDRPSVALVTYNNKEDGYKQNIEYVEDQDAMRRY
                     GERKTEVVAFGCTSRGQAHRVGLWLLYTARMESDVITFTAGLDASFLMPGETVLIQNK
                     YRAGKRNSGRIVAFTKNSVTLDAPVSLAKGGCFIRILNQEGKIVERDVLETGENITKV
                     TFSKALSSAETPVLNGVWTITEPDLEPMRVRIVNIAQGETPGSFDITAVENNPSKYEA
                     IDNGATLIPQNTTVLDPTYSKPSNLQITEGTYLSSPGNLSVKLTATWEGKSPEYWISW
                     RRSDENNVSNWQSARVTEEQYEIVNVAENGRYDFQLYAVSFNGKKTEIISAVYQVLGT
                     MTPPDSPTSLTAVGDYRNVILNWVNPDSVDLDHINVYASQTNNLDTAKLIAESASTTF
                     THAGLGDSETWYYWVRASNKRGMLSPPNSNLGTEATTRDVLSFLTGKITSSELGQALL
                     EDINSKASQEAVDELNEHINQSVESLEGEVNDVKEDIAELDKQFSDNLADFEIKFNER
                     SDALENAQTELKGEVSATIDKVNEAFEKIDATDAAIVEIENTVSEHDKALANTVEAIK
                     AARDEAAALIAKESEARVEGDAANAKQLEVLQSTVEESSAAVEEMKKTVAEVDRASAE
                     LTTNIEALAKTNIDLALRQDEDQHKQMVNNAKIATTQKTFADDMSAMATKVEEIRVEI
                     GEDIKASILEESTARADGDEALAKRVTQLQSKFEGDISAAISTEQEARTSADEALTTQ
                     ITQLESKVSNDIVAAIKEEQEARATEDSALASQITQLQAKVDDDISAAIRSEQEARAS
                     GDSALAKQINQLQSKVDGDISAAITQEQEARASADQALSREINSLRAQTGTDIAAAVA
                     VETKARTDADSALSTQITSLTAKANDLEASLARETTARADGDTALTKEVSSLKAQTAK
                     DISAAVAVETQARTDADSALSSQITKLTSQYKEDIKAAVATETKTRTEQDAALATQIT
                     NLESQTAANISAAVTTETTARTQADNALSGRIDTLKAEVDGNTATIQQQATAIADTNK
                     KVSTAWTLKMETSTSGGQKYVAGIALGIDTTGLSQFLVQADRFGLVNSVNGKITTPFV
                     IENSIAYMNGAYIKDGTITNAKVGDLQSTNFVSGRSGWRFGKNGTLEINGNSGGNGRL
                     VINGQRIDVYDDNNVLRVRIGRL"
BASE COUNT         8077 a         6370 c         7472 g         7175 t
ORIGIN      
        1 aaaaaagatt gcaagtgttt ttttaagttt ttttgcaggg atgaaagtcc tagaaataaa
       61 agcgagatcg tcgaaggtgt tccctaaata aataataaat tccggctttt gaccttatat
      121 atttatacgg gggagaattg aatgagtgtt aaacgaatgg catgtaaaat aataaccgga
      181 cttagccggt tattaccctt atagaaatta aaacggaagg attcgttcaa cccaatcgaa
      241 gaaaataagt atttttctac cgtcttccaa cttaagtgtg actagacagg cgtcaacgcc
      301 atctgacgtt ccctcaattt cgcgaccgtc tgccatgtag acccttatag atttgccgtt
      361 ttggtaagcc tgacgacata tcttaaaaaa gtcacgacgt gatggctgat taaccatctg
      421 ttcattgttt agcgttagtc gacctgtgaa tgaagtgtca acctcttcta tgcccgactc
      481 aattgtgcta atgcgctcaa gagggagcct tatacgattt gtgttgctaa gcggatctgg
      541 tgtgaggttc agcttatttc ttgcgctcaa tagcccgtag acatacatgc aaaacacttg
      601 gccgtcttcg agagtgactc ttacaggaaa ttttgcactc cgccagaaga gcaaggtgcg
      661 ctcagcatgt tcgtagtctc gcggccagac ttcagccggt atgccgtagg tgatgtccaa
      721 tgttctcatt ctgatactct tagaggtgtt ttcagcaacc caaatttaaa ttctttgtta
      781 ccaacgacaa agaagggaac ctcaacatag atttttttat cgacggattt agagttagct
      841 gttttatgta tgtcacgaac gatacgtttt gagatatttt catcgataaa caataccttt
      901 ccacttgtat tcccttcagc cttggcgtaa gtaacgatag gctcttcatt gccaacccta
      961 atgtttactt ggcaacctgt tgtagccatt tcatcacagt aaagtgtgcc gtcttgaact
     1021 tccaaaacaa ttccagatgg cttcatggct tcgtcagagc ttacattaac gttttctggt
     1081 acagcggtcc cgttaatttg caaccgtaat ctttcttttc ctgcattaag tattgtttct
     1141 tctgcgttat ctgagaatct gaaccagact ggttgaatca gacctcgtga ttcgtatttt
     1201 agtacgtctt cagaagtata catgctgtac gttttttgca tgtcggagac gatatctgac
     1261 aacgcatttg cggaaaatga cgagaggtat atacaagcaa gaagtaacga acgtttcatt
     1321 tttatacagc gattattgca aagtagaatt ataaatatcc aatagtctca aacgataagt
     1381 tttcaactgt aaatagcaga cgtgtttctc taaaagctgt gcggcaaagg cgttgttgcc
     1441 gcactctgtc tattagctct tcaagtctgg caattctgaa attgactaag aatccaatat
     1501 tacggcagtt tgcttagaat ttgctctaaa tcttcctttg tcatattaga gttctcatag
     1561 atgcgcatga ttttctcacg agccttagca gagactccaa cggcagatga aactttgtca
     1621 aattcagcca ttgtcatcgc ttccagaatg gtattgataa cttcagcctt agacattttg
     1681 atgttttttt cttttagttt catttgaaac tttccaagtt tgtcattggc cttatcggac
     1741 aatgccacct gacaataagt tgttttcttt tcgctcataa ctaatctcgt ttcagaactc
     1801 caaaatcgaa tgcgccatca ataggcaata caccttctgc aaagccaggt gtggtgtcga
     1861 tgatgtgttt tcgctcataa gagtgagaca acaggtattt gttgctaatg tcaatgaaat
     1921 cagtgataaa acacacgttt gcctgatttt ttttggctcg taagccacga ccgacacgct
     1981 gtctcatttc aacttctgct ttcccaccac cagcaagaat gaccgcacca acgcttggca
     2041 catcaacacc gacatccaga atagtcgagc ctattaaaac atctatttct ccagacgcta
     2101 aactgttcag ctttgcttgc cttgtcgcct ggttagattc cccatagatg aagtcaactc
     2161 taaggccgga ctctttcatc atttccatca ggatttgccc gtggcgttta agacgaacca
     2221 gagtcataca attgagagaa tgttgcttat agagcaatgc ttcgcgaaca atggcctcgt
     2281 tacgtcccaa attatacacg atccccaact gataagcctt ttggtaggcg gtgctcatac
     2341 caactctaaa attgaggtgt ttgttggcaa gttcggcctt gattctggcc tcgtctggct
     2401 tgtaggcaac tttatgataa aggaagtacg gctttgccag aatgcctcga tcaatcaggt
     2461 acttttctgt gactttaatt tcaattcgcc cggccaccgc catcaggcgc atgttggctt
     2521 ccgtcgaatc cttcatgaac ggcgtggctg taagcgccag acgatagtct gcgttcacac
     2581 ataatctggc gatgtcatag aaatttgagc ctgaagactc atgcgcctct tccagaataa
     2641 gaagagagac acttgaaagg aagcgtttca ccaactcccg acgtttgagg tggtagctct
     2701 ttttatctgg tgttgcatcg cgtggtggtt cttcgaggaa acttgcaaga gtttgaactg
     2761 tagcgacgtt gatatgtcgt gatacctgga actcacccga gccaatgact ccaacctttt
     2821 ggtcttttaa ccacggttcg ccattttcgg cgcggtagtc gatggatctc tggaagtttt
     2881 cggccatttg aaacatcaga acagagcggg ttgttaaaaa taatgtcatt cgaccgatac
     2941 gtgcagctgc tttgcaggca acattagatt tcccgccacc agtagcgatc tgcgcaatca
     3001 tcattccctc tcgaaccagt gtttccacag tctgatcctg atacgcataa tcaggattgt
     3061 atgggaatgg gttaaccgcc ggatttggtt ttccaagcgc cggggctttg tctttgcgga
     3121 tatgaacaca tttgatgccc gccttgttca agttcgccgc tacaggcttg gcaaagcctg
     3181 cagggaacga gtttttactc cagttgaaca ttgtgcttgt gcccttccag tcgccagtct
     3241 cgacttcgta gctcaacatc tgttgcacca gttgctttac cttgtcatct gcgccagaaa
     3301 taagcgcatt aactgcgtta gatacaatcc gaacagtcat aaacctcttt ccttagtgcc
     3361 ttttgtatgt tatttggcta ttataataag taagtgatta cttagtggat tgtagcaata
     3421 aaatggatgt aaaaatcacg attttgcagg ttgatgttgc caaccttcgc ccgaatacct
     3481 ggaacaccaa ttcggttggt gcgcagaatt ttgaaaaact gaaaggttct atcgaaaaat
     3541 tgggcttttt taagccaatt ttggctcgtg aacttgaaga tggatttttt gaaatcctcg
     3601 gcggcgaaca tcgctggcgt gccgctattg agcaaggaat ttcaacggtt ccagtgcttt
     3661 ctgtgggcaa aattagcgat gtcgtagcta aacagatgtc actggtggac aacgagcgat
     3721 acggtgaaga cgaccaaatc gcattgcaac gcttcattga agaaattcag tcagaacttg
     3781 actatcaact gtctgaaatc gccccgtatg acgacgaaat ctcgatggtt ttagcaaaag
     3841 aggcggcaat cgaccttgaa gcactggagg cgttgtctcg tggtagtgat gagcctgtcg
     3901 ataccgacaa acgagagaaa accgaacgtg tgggggcgga acatcagacc atgcgcttca
     3961 aagtaacttt cgatgcgtca gatcgcgttg cagaaaccat aaaaaacatc atcaaagagc
     4021 aggctattaa caccggtaat gaaatggaga acgctggtga ggctctggtg tggctggtcg
     4081 actactacaa ggagcgtatg taatgaccaa aaagtttgaa atcgtatatc gcgacccggc
     4141 agatcttatt ccctatgaga tgaatgccaa aaagcatgat gaacagcaga tccgagatct
     4201 agccgcagcc attaaaaagc gcggatttga ccagccaatt acggtcgata agaacgacgt
     4261 aattattact ggccacggcc gccgtgaggc tgcaattttt gctggacttg agcgcgtacc
     4321 ggttattgtt cgcgatgatc tcagtgatga cgaggttcgt gcgaagcgcc ttgaagataa
     4381 ccgacttgcc agcattgatt acgatgcaat taagctacag aaagagcttg agtcgcttgt
     4441 tctggacgat atcgaggttt tcggctttga agagcgtgag ttgaatgttc tcgttggcag
     4501 catgacagaa gaaatggaca ccgactcgct agttatcgat cttggcgaag aaactaaacg
     4561 acagaaggat gaacacaccg aaatcagtcg tgaagttgca gcggaagaag tacgtgttgt
     4621 cgacgtattg ggctttaaaa cgcttcctgc tggctctgcc attgttgttg gtgatttgct
     4681 tgcccacatg gaagaaatga cgggagaaag cggggtagac gcatttgtgg catatgcgga
     4741 gaagatctct tccggggaga tggctgcatg agcaaataca tcatcaacgt atcgtttcag
     4801 acacgcgtaa ataaaaccac gcgcacgttg gaaatcgctg agtcgttcgg gcttggcctg
     4861 gacgaaaaag agtggacgct ttacgacaat ctggagctgg aagtgaagca gggcgatgtg
     4921 gtgtacatca ccggccaatc cggttccggc aaatcagttg tgctgcgcga gttgcaacga
     4981 cagatgaagg atgaagggct gtctgtagcc tccatcgatg attttacctt cgataatgag
     5041 gttaacgtca tcgatcagtt gggcaaaacg accagcgatg cgcttgggtt gttatctatg
     5101 gctggtctga acgatgcata tctgtttgtt cgcaagcctt ctgaaatgtc agacggtcag
     5161 aaatatcgtc tcaagattgc caaactgatt gagtcaggcg ctaaagtgtg ggctgctgac
     5221 gagttcggtg ctgttctaga ccgtgtaacc gctcaggttg tggcatctaa cctccagcgt
     5281 gccgctcgaa aggttggtgc aacggtaatg gtggcgacga ctcacgaaga cctgaagaac
     5341 gcgctgcgcc cggatatgca gatcaccaag cactacaaag aacgcgtgaa ggtggaatat
     5401 cacaatggta gtcatgatga ggttcattta tgacggacat catcattaaa cgctaccgcc
     5461 ctgaagagta tccgcgtcat ctggactttc tggagcgaat gactgtcaca aagggaactg
     5521 tagaggactg gtacgctctt aagtcgcttc actacaaaac agacggcaaa cctttcgcgc
     5581 caacttacta tcgctgcgaa cttgatgacc gtctggtggg cgtcgtggtt atggcttacc
     5641 cgaaactact gttggcacct cgccaccgca tgtttcctaa gttgaaacca accactaata
     5701 ccaccgtggc taaccagtac tggggtcggt acgtgaataa caactttgcg gtgatcagtc
     5761 gctcagttgt ggatactcag tatcgtggcg taggcgtctc ttatcgaatg attaacctgg
     5821 ttagcaggat gcatgaccgg ccaatcattg agatccagtc ctcgatgagc aaatacaatc
     5881 ccttcgccat gaaagcaggg tttaagttca tccgccctga gcgaccgaag agctatgaaa
     5941 gtgcactgcg tgtattccag cgccatttcc gttccgaccc gggtgataac gaggcgatcg
     6001 tcaaagagtt gttcgcaatg agcgagtctc gtcgtcgccg tgcactgcgt gatctggtag
     6061 cggactacca caagaacagt tccctggcaa aagctgggcg gaatcgtggc acgacgattc
     6121 aggacattgc cgacagtctg gtggacgagg ccagcattgt gaagctgctc aaggacattc
     6181 acaacctgag ctttacgtct ccgttgtatg gtgtgtaccg aaaccctgac tttggtcgtc
     6241 ggctgcctga cacgctgcca ctgctggcat tcgacaaaca acctttggat aaaccgttag
     6301 aaattgcttt accggcataa ggatttgcca tgacgttaac cgataaacaa aaggacatca
     6361 tcaaaacgct caatctcggt tatgagcgag gtcatttact tgatctggac gaattgcttg
     6421 aagttttgcc gtacaagaca accaagcaaa gtatccagtt ctcaattcgc gctctgataa
     6481 aaaaggggct ggtggagaaa gggcatacgc gccaacgcag tgacaatcgc tatcaccgcc
     6541 gaactcttgg gttaaccact ttaggtcgag ccaaagcgaa gttactggtg atgtaatcgg
     6601 tctgggagct tatttaaaga cctgcttctg tatatataaa taataagtaa cttattaaat
     6661 atatacggaa gcaggcttaa taagacatgc ccagacctaa ttaaacaccc cagaaaacaa
     6721 gttggttagc agattcagta aacaagttgt tttagagcgc atggacgcgc tctgtgtgtt
     6781 ttagagggat ctatgacggt cgaaaaagac gaggtaaaaa ctcgcctgac accagcggag
     6841 tgggccgaag ctgaagccaa atggacgtca ggcgaatata cactctcaaa gctggaggaa
     6901 gagtacggca ttcgtcgtga aacactctcc agacatttca aaaagcgagg attagagaaa
     6961 ggcgcggact ctgttgggaa gatggttcgt gagtctctta aatctgacgc agagcttcgc
     7021 gctaaagccc gtgcggaaaa gatagaagaa cgtcgtacac gttatgacgg ctgggcgtat
     7081 gcgttggggc agatggtgat ggtcgaagtc actacggcca aacgtgaggg taagccttta
     7141 ggggcgattg aggattctct caagagctta cagagagcca gtaataccct tgcaaaatgc
     7201 tttgaagttt cgtccaaagc attgggcatg gatcatgcgg aaaatgacga ggaagaaatt
     7261 ccgaacctgg tatttggtga gcttacgcct tcccaggtgg cgaaattacg tcaggaagac
     7321 gacgagcctg aaatcatcga tgacgaattg cttgagacgt tggaagaaga agctctaagc
     7381 gaatttgatg cgacagatga tggaattgaa ggggaggacg aataatggca atcccgtcct
     7441 cgctcagtct tgtgcaactg cattctggac agatgaaagt cttccagtct ccgcatcgat
     7501 ttaaagttgt ttgtgctggt cgacgctggg gaaaatcccg gttgtcgatc tccactatta
     7561 ttcgtgcggc ggcaaaggaa aaaaagcaaa gagtctggta tgtcgctcct acttaccaga
     7621 tggctcgcca gattttgtgg gacgatctac aggaagttct gcctcgtcag tgggttagga
     7681 aaaagaacga caccacgatg acaatcgtgt tgaagaacgg ttcggagatc gccctcaaag
     7741 gtgctgataa gcctgacact ctgcgcggcg tagcgttgca ttttgtagtg cttgatgaat
     7801 ttcaggatat gaaggctgac acctggtaca aggtgttacg acctactctt tcatcgacac
     7861 gcggcggtgc actgatcatt ggtacgccaa aaggcttctc ggaatttcac aaactgtgga
     7921 ctataggcca gaacgtagag ctgcaaagaa agggacagtg gaagagctgg cagtttgtaa
     7981 ctgccgattc tccgtttgta cctacggcgg aaattgaagc tgctaagaac gatatggacc
     8041 cgaaatcgtt cgctcaggag tacctggcga gctttgagaa catgtccggg cgcgtttact
     8101 acccgttcga tcgtaacgtg catgtaaaac cgcttcagtt caaccctcgg ttgcctatat
     8161 gggtagggca ggacttcaac attgacccga tgtcttcagt aattttgcaa cctcagccaa
     8221 atggtgagct atgggcaatt gatgaattgg tgctcttttc ctctaacacg gcagaggttt
     8281 gtgatgagct tgagagacgc ttctggcgct ggaaatcaca gataacggta tttccagatc
     8341 cggcaggtgc ttatcgccaa catgctcgcg gggagtctga cgtcgacata ttcaaagaga
     8401 agggattctt acgtgtcgat tattcgaaaa agcacccgcc aattgcggat cgtgttaatg
     8461 ctgttaaccg aatgctgatg accgcatctg gagatatccg gctgtatatc gatccgaagt
     8521 gcaagcattt gattgattca ttggaaaaag tcatctacaa gcctggaaca cgagatatgg
     8581 ataagacagg tggcattgag catagtgcag acgcattggg ctatccagta catcgtaggt
     8641 atccagtcaa aaaccgtgtt attcttggtg gttctcgata ggtaagtaat tatctaaggt
     8701 tattcaaatg gaattgaacg acaaacaaat taaggatctg gtggcgcgac gccacccgga
     8761 atacgaaaag aaaaaagaac attgggactt cctcgccagc acttacgctg gcgggcgtgg
     8821 ttggtttaca gacaatatct ttcgttactt taaagaggga gatcaggagt ttaaggagcg
     8881 agttgagcgt gcttatcgct tcaaccacac tcgtgaggtg gtaaacctca tcaacaaata
     8941 tctctttaaa gaagacattc atagaaatat cgaagaggca ccagagcaga tccgcaattt
     9001 ctggaaacgt gcgactcgcc agaatgcctc tattgactca tttatggccg ctattgattt
     9061 gcagtcgtct atttacggtc gcatatgggt tgttgtcgat agcacgatga gtggtgatgt
     9121 tgagtcagta gctgacgaga aaaagaaaga tgctcgcgcc tacgcctact ggatttcacc
     9181 tcagcaaatg ctggatgtgg catgggacga cgacgggaat atgttgtggg cgttaattgt
     9241 ggaagtcgct cgtgatgacg cagatccttt tacttctaca ggtcaggaat accaacgtta
     9301 tcgtctgtgg acacaaaacg agtggtatct gttccgtgag gaagtgaaga agggcgctgg
     9361 tggagcaggt cgccgtcagg caaaagttat tttagaggat agcggtgagc ataatctcgg
     9421 cgtagttcct gtgtttcctg ttgattgtat tggagaaagt gaatcaccgt atttcagccc
     9481 atcgttgatc gatgatatcg cttatcttga tcgtgcggtt gcaaactatc tgtcaaacct
     9541 tgatgccatt attcaggatc agacatttag ccagttggct ataccggtac agtcgctttt
     9601 acctggtgat gaaaaccaca ctaaagtgct tgaaatgggc acaaagcgag tcttcaccta
     9661 cgattctgaa ggtggaaacc agccgtttta tctgtcacca gacccgaaac aagctcagat
     9721 gatcatcact acgattaaga cggtgattaa cgaaatctac cattcagttg gtgtagctgg
     9781 tgagcgaacg aagcaggaca acgcacaggg aatcgataac tcatctggtg ccgcaaaaat
     9841 gtatgacttc cagcgtgtaa atagcttgct tgtcacaaaa gcagagcgtc tggaaagggc
     9901 tgaacgccaa atcatgctac tggttgcgaa atggatgggg gtagatctgg acgaagacca
     9961 ctcattaatt gcgtatccag aaagtttcga tattcgtggc cttactgatg aattttctgt
    10021 tgccgagaaa ctgtcgttac ttcaggcacc ggactctgta cgtcgtcacc agatggaaat
    10081 gctgattgag aagattttcc cgaacatcac tgaggcgatg aagaaggaat ttgataaaga
    10141 tctcttgaat tttccgccaa aaaatgatct aaatactctt gaaaataagt cagtacttac
    10201 ttatgatcaa ggcgcagccc aagaaagcgg gcaagatcaa ccccgaggga atggggactc
    10261 atctactcaa gagaacgagt gataagtaac aaagggaatt tttatgcaac tgtggcaaat
    10321 gcttttggcc cgtcgtggtc tgatgggtgt tgctgaagcg catgagcgtg gaggcgctgg
    10381 cggtgtagct gctgataatg agcagagtac acaagatcct gacaaacagg gtgaacaaaa
    10441 agagcagccg aagggtgatg acgaatacgc tggcatgact catgaagagt tactggccga
    10501 acttcgcaaa accaagaaag ctggtgctga actgctgaaa gagaacatga agcgcaaaga
    10561 gaaagagcgc acattggccg atcagcttgc tcagtacggt gatatcgacc cggcgcgtgc
    10621 tcgccagctt ttagaagctg aacaggccgc agaaaatgca cgtcgtgagg cggagcaagc
    10681 tgaactggag cgtcgtggtg agttcgatgc tgttaaaaaa cagatgatcg aagcacacca
    10741 ggcagagctg gcacagcgtg acgaacgtta tacagcactg gaaagtgaaa acgcatcact
    10801 gaaatctcaa ttagtcgaga tgaccgtggg cgcttccttc agtaactctc tcttcctacg
    10861 tgacaaagtt ctgatgaccc cggcaaaagc ccgcgtgatc tacggttctc attttgaagt
    10921 gggtgaagac ggtagcgtag tgggttatga caaaccggca ggtcataaag aacgagctgt
    10981 tctggttgac ggtgaaggta agccgttgcc gtttgaatcc gcgattgaac gcattgtgcg
    11041 ggcagatccg gaagctgacg cattgatgcg tagcgaagcc aagcagggtg taggctcaca
    11101 ttccaaatcg acctacacaa tatcccaacc gaagaacaag tcgactatgg ataagttggc
    11161 ctccggtctg gggaaaattg gacttaagta acatctaaat caaagggaat tgatagatgc
    11221 cattactgcg tgaagaagct gaaaagctgt ctaacaacga actcgaacag ggtgtgatcg
    11281 aaaccatcat tgatcgcgat gacctgtttg ccatcctgcc ttttatgaaa attaattcaa
    11341 aggcatatct gtacaaccgt gaaaaaacgc tgagcgaagc tactttcatt gatgtgaacg
    11401 acaccatcac cgaaggtgca gcaaccttcg aagagaaagt tgcgaagctg cgcattctgg
    11461 ctggcgacgt tgacgtcgac aaattcctgg ctaccactat ggctgatacc aacaaccagc
    11521 tggctatcca ggttcgtcag aaagtcaaag gtctggctcg tgccttccgt cgcaatctga
    11581 ttgttggcga ctccaccact aacaacaaag ccttcgacgg tattccgaag ctgatgcatg
    11641 acgatcagaa gatcgacatc tccggcgcat ccatgacttt ctctatgttc gacgaactgg
    11701 tcgacgcagt taaagatctg ggcgcagact gcatcatgat gcgttctgag catcttcgcg
    11761 catatcgtgc gctgctgcga actgtaaacg taggcccgtc cgaaatcatg atggaaaact
    11821 tcggtcgacc aatgctgtgc cataacggcg ttccgtttat cgtaaacgac ttcattccgg
    11881 ttgtggactc caccaaagcg gatatctact gtctgcacct ttctgaagag aacggcgtta
    11941 ctggtctgta tggcggcgac aacgcaggta tcgttgttga aaacattggt actgtacaga
    12001 acaaagacgc agtacgtacc cgtgtgaagt ggtactgctc tctggcgaat aagcacgata
    12061 aggctatcgc tgcactgacc aatgtaaaaa tttaatcagt gtggtagata agtaattatc
    12121 tatgtttaag ggtgggctat acgcccaccc tttttgtagg agcaagaaat gccagaacaa
    12181 aagatgaaga ttacggaaga ggcattttcg gattttacgg ggcatatgtg ccgcgccgga
    12241 tttaccaatt ctatctccga tgaaccttta accgagcggc aacaaagtca gctatctgct
    12301 tgtttgcagg cggttccctt ctcccaatcc gtaaacataa ctccggcttc accatcagtt
    12361 ttggttggga aaactgttca acttagtgca ggtattagta tgggtaagag tgccagttca
    12421 tttacctgga agtcagccaa tgatcaaatt gcaaccgtta acggcactgg attagtaact
    12481 ggcgtagccc caggcaaagt aaaaattact gcaactgatc aggaaaccca gctttccgct
    12541 tcagtggaag tcaccgtaaa tccggtagcc gttcaatccg taacggtaac gccagaatca
    12601 acgtctgttg agaaagggaa atcagtcagt ctgaaagcga atgttcagcc gtcaaatgcg
    12661 acgaataagg ctgttacctg gtcttccaaa aatgaagata aggcaacggt agaccagagc
    12721 gggaacgtaa ctggtgtaga agttggcact gcgacgattg agatcgtttc ccaagatggt
    12781 agcaaaaagg caactgcaac ggtggaagta actgcgcctg ttgtggctgt tacaggcgtc
    12841 gaaatcgacc caaatagcac aaccgttgag gcaaacaaaa ctgttcagct gaccgcaaat
    12901 gtcgagccgg caggagccac aaataaaacc gttacttggg aatctaaaaa taccgagttt
    12961 gcaacggtgg acagcgaaac cggtgttgta actggtgttg cggctggtac tgccacaatc
    13021 gaggttacta cccaagatgg tagccacaaa gcgacagcga ccgtagaggt cactgtcgca
    13081 caggaataac cgacattggg cggcgaaagc cgcccaagag gagagaaagc gttatgaaac
    13141 cagcaaaaat tgttttatta gaaccgcaat tttccggtta ttcgggaatg ctgtgcggcg
    13201 ttcagttcga gaacggggtg tccgtagcgg agttgccttt tatcgatcag caaaggattt
    13261 gtgcctcaat gcgagcatca acagtcgagg gcaaaaatgt ttctccgtct gccgcataca
    13321 gtgatcgtgg cgagttgacc gcagacctga ttaccgagcc agcagcgcct gacattgtgc
    13381 caatgaaacg tgggacacca gatgaaccgg ccaaacagat ccagactttc acacgagaag
    13441 agttggagtc aattgcagac aatgaaggca ttgccggtct acgtgtgatt ggtaatcagg
    13501 ttggtgtcaa agcgaaagga attgtcgaga tgattgaagg catcctgaaa gcacaaggcg
    13561 gtgagtaatg gcgcagatcg actcgtatcg tagcggtgaa gctgtttccc tttcatttgc
    13621 cttcaacgtg ctggatatcg aatctgctac ctacacagta aaggacagca ccggggctat
    13681 tctcgttgat ggcgagccgc tagaagttac cagcggtcaa atgtcgattc cggttgtcgt
    13741 gtcggctgaa tataaccagc tctctgagaa agagcgagat ctgcggtacg tcattgtgaa
    13801 ggctgttgca tcgggtctga cgcatgaaga gcggcaaatg tatgttctgc tgaatagttt
    13861 tgaactgtcg ataccagaac agtcgtttgc aactgtcgct gatgctcaaa tgcaggcgat
    13921 cgatatgctg aatggggaca ctttgctgtc ggatggtgaa ggcttaatgc gcaagcgtct
    13981 cattgaggct accagacgaa ttaaaacttt accgttctca atccgcaaaa ttctacgtat
    14041 cgactttgac cgatacgatc gccctcaaaa tatgctgaat gtgtatgaca ttccgtgggg
    14101 agcagatgga gcgtaccggc atgatttagt cgattgggaa aagatgacgc aggagaagtt
    14161 tgaagagttc cctgactact tcaaagaagc gttgatgctt gccgttgtca acgaagcgtg
    14221 tgagatcgca aatggcaatg atgtagccgc agcacgagag gatggcattt tgtcagagtc
    14281 catcggtgaa acaaccaaca tgtaccgtac cggtaaagct gcaaacgtgc atgtggctcg
    14341 tagtacctgg cgcttgctgg tcagttacat caacaaccgt atgattgttc gccgtgcgta
    14401 acgccagtcg cattatttac ttttggtcta aagggttttt ggccagtaat gagagcgacc
    14461 gacaacaaca gggagagagc atgaatattt catggcaaac agaaattgcg atctaccgct
    14521 ttggcgcaaa gaacgtttac ggtgaggcgc aattacaatt cgtcaggaag acgaatgtcg
    14581 gcgtggttaa gttcgaacag agtaatgaga aatcgtcagt tcgtgctgat agctctggta
    14641 gtcgtggtaa ggcgagtctg gagctgtttg atgctgtgtt agttgtccct ttagaagcgg
    14701 ctgtacaact tgatgatgtg ctgatccttg aaggtcaaaa attaaaggtg tccagtgttc
    14761 atcgccgctg ggggctacga ggaagacctg ggcatcttga agtgggggcg aatatatggg
    14821 tctgaaatac gatgcgcatc agttcaaacg tgcggggaat agactcaata acagccagaa
    14881 agcatttaag cgatatctta ttcgggacat ggaaaagctg gcgcgtttgg ttgagcgtct
    14941 cagccgtgca atggccccgc tggaaaccgg atctctggag acagccatct ttgccagggt
    15001 gattaaagaa ggttattcag ggctgcgcat tgagctttct gtgtctggag caaaaccacg
    15061 tcaggggcat ccgggcgtag aagtcggtga ctatgctaag tacatggagc tgggtaaata
    15121 ccgactcggt tatctttctc gaatgaagaa cgtgacaaac ccaccaattg ccggtgttaa
    15181 acctcgtgtt gggccacact ttctggaaag agcggtggaa attagcgaga agcagttctc
    15241 agaagcgatt cttgaggctg caagaaaagc cggttttacg agaggttaat gtgtttatcg
    15301 aagcgtttgc aagtttgatg cagaaggcaa agattggcac ggtcgggact gacattttct
    15361 gtcactactt gcctgccaat gtgaaatctg gcgtcctgct tattaaccca aataccggta
    15421 tcagcatcga tcatgagcta cagggctttt atcacgaatc attcacaatc attgtgcgtg
    15481 gttcgtcaat tactacgacg gttgagaaag ccaataaaat cattgagatg tttccagtag
    15541 aggaaacgga atctggtggt gtttattttc gacttgtacg accgatggcg atgccaatca
    15601 tttatcccaa aaatgatggg gcattaatag aagcaggcat tcctgttgaa tttgctggct
    15661 atttattgaa ttaactcaat tggtaagtat atacttacta ttagcgcaat gaatgcgtag
    15721 aattaacgga aaaaggagtt ttccatcaat gtctaatacc catgtaaaaa acatcaaact
    15781 tggtgcctgt aaggtgtcgt ttggtggtgt ggatttgggt tacaccaaag gtggtgttca
    15841 ggttgaagta gccactgaaa cgctgaaagt caccgtcgat cagttggggc agaccgtaat
    15901 ttccgaatta gttcaggggc gcaacattac catcaccgcg cctctggctg agtctgtact
    15961 ccaaaacatg gttgacctga tgcctggttc tactctgagc gatgatgaca gctctgtcac
    16021 gatcacatct gcacagggcg ttaacctgat tgatgtggcg aaggagctgg ttctgactcc
    16081 gcaggacacc accgactatg tgttgaccat cccgaaagcg gcaaccgcgg gcaacttcac
    16141 tatgacctac cagtctgatg atgttcgtgt gttctctgtt cagttcagcg catacccgga
    16201 tgatgaaggc gttctgggca aaatgagcgg tcctaaaccg gtaaaaactg tgtcgatctc
    16261 accggaatct ccaaccgtaa aagctggaga aactgtgcaa ctgacagccg aaatcactcc
    16321 agctgatgcc gcagacaaaa ccggtgtttg ggaatccgaa gaccaggaaa aggccacggt
    16381 agatcaaacg ggtctggtac gtggtgtggc gcagggttct acaaatatct ccttcaccag
    16441 caatagcggt ggtaagaagg ctactaagtc agtaacgatt aactccgcag gttaatcagt
    16501 aattaagcag aggctcagga tgagcctctc ttttaaaagg attttaacca atgaccaaat
    16561 tactcgatct cgattccatt ctgcctccga agaaaagcat caaatttggc ggtcaggaat
    16621 atcccatcgt tgaaatgacg gtaggtctgt tcgtttccat caagcagatg gaaggcaaag
    16681 atctcatgaa catgtctcct gttgagcaag tgacagctta tgcagatctg gttcgtaagg
    16741 ttatcccttc agtacctgac gaagttcttg aaaaactgac tgttccgcaa ctccagcaga
    16801 tcttcacctt cgctatggaa gtgattgatg aagaaaacga aaaagcggct ggcgaagggg
    16861 caaagtaatt tcccgcgatg aatccgggac aaggaccgtc tcaatagatc tcggattcta
    16921 tttcagtcgt gtagttgctc actacgccgt gtcgccatta gagctactga acgttcctct
    16981 cacgatgttc tggatgctca gtcgcaatat agaccgtctg cgtgcggaag aggatgtccg
    17041 caacttacaa gtcgctcgtg cgtcccaggc ggatggcgag gctgtgaagg cgttcatgga
    17101 gggtttgcaa ctcaggattg gaagaccagt cgtaacagat aaagtctacg atccaagcca
    17161 ggataaggca gaccctgacg ccaaagagca actgatgcaa atatttggca gaggatgaca
    17221 agggaatgtc acaaaacgta gagtttatcc tgtcgctgga agacaaacag tttacagcgt
    17281 caatcgatcg tgcggggaaa ctgcttactc gatttggtga gcaggtaaca aagcctgctc
    17341 aaaaaatcca aacctttgaa cgctctttgg gttcggtcgc ccgtatcatt ggcgttctgg
    17401 aaagcaagct cgattctacg gcagataaac tacaggatgt agctgccggt tttgagcttg
    17461 cttcagatgc tacgcgtaaa atgcgtggca acatcaccag cctcaattct ggtcttaaag
    17521 ccctgattga gcgcgtcgat acgacaactt cttcagtaga taaactcacc gcgtcattac
    17581 gtaaagtgca atcagagcta aatgatttct ctgattgggc gacctatgca agcaaaagcg
    17641 caagccgctt tggtacggag gtcaaagaag cctctgtgtc cgtgagtggc atgaatacgc
    17701 gccttaatac cacgacgaag cgactcagta attggggtgt cacaacgagt caggctgccg
    17761 agggactgaa aaaggttcgt gagcagatgg acgaagttat cggacgtcag caactgatta
    17821 gcaagccggt acgcgttcgt acatctggaa gtggcgatgg tggtagcggg cggcgtagcg
    17881 gcgcttctgg ccatagcggt aaaagtaacg aaggaggtat gttctctggc cttcgtggca
    17941 acatttttct gcttggcgag atcggagatg cagccagaac ggttactgac atcatgtttg
    18001 ggtggcagaa gcctattgtt gaagctgcgg ccgaaatgga acgtatgcgg gtgatgcttc
    18061 gagggttgaa taaggagaag tccaacccgg gccaggctgc cgctgatgat atgaagtaca
    18121 tcgtagacat ggctcaaaat gcgccgttcg cgatgcaggc gttaacagac tccttcgtga
    18181 agtttcgttc tgctggctta gatccaaccg atggttctct gaaggcgctg gtggactccg
    18241 ttgctcgttt tggtggtgat agtgagctgt tgaaacgtgc ggctgtggcc gttcagcaga
    18301 tgtccggtaa gggcgttgtg tcaatggaag agctacgtca gcaattaggt gaagccgttc
    18361 ctaacgcgat gcaggcaatg gcagacgccg caggcatcac tatgggggaa ctgactaaag
    18421 ccgttgccag cggtacggtt gaggcaaaac aggcgttgtc tctgatgttt gttggtctgc
    18481 gtgcggagaa tgagaacgca gcgaaagaca tgatgcaaac ctacacaggt gcgctggcgc
    18541 aacttcagac gtcattcacg ttatttgctg atcgggttgg tcaggccgga tatctggatt
    18601 ctctatcgaa ggggatgaaa gaactggctt caatcatgaa tagcgccgaa gggatttcgt
    18661 ttgccaattc tttaggttcg gggttaacaa cggcaatcga tgggttgcgt cagcttgctc
    18721 aatggttagc aaagaaccaa gagctggtaa ttaatctcgg taaggtcgtg gccgcgatgg
    18781 ttgcgttcaa actgatgcga gcagggatca tgggggtagt tggcgcaggc agccagatgg
    18841 ttagcacctt tgccacgatg gcgaccgcca tacagactcc atttaacctc ggcgctacag
    18901 cagtaacccg attcaatcgt gcggcacgta tggggctggc tccgatcccc tctcttattt
    18961 tcgccatccg tggggcgatt acggggctta aaggcgcttt tgctggatta acggcgttca
    19021 ttgcagcaaa ccctataggg gcagcattta cagttgccac tgtagctgtt gctggcctaa
    19081 tcacgtacat gaccatgctc cgcagcgaaa cttcaaaggt cgttgacgag attaggaaaa
    19141 taccagaggc gatgacggcg gccaaacgtg cacaaatggc cgaccgcgca gaaaagctcg
    19201 aaaagcagat tcttgctgat caacgagcat taaaaactgg tgagggtgtt aattacgtat
    19261 ctaactctgc cggtgttatt actcacaaag agtcgaaggc tgacatcgaa gcacgcctga
    19321 aaaaaaatca ggaagagtat caaaagataa ccggcacgat agctcttggt gacggcgctg
    19381 tagcaaagcg tttggctaaa gaggctgccg aatctcagat tgagaaaatt cgagcggaaa
    19441 accagatttt tgcggcaaca ttcgtgaaag cccggcagga ggctctggac aagatccaga
    19501 aaatcaatga tgacggttca ctttcagatg acgaaaagaa caagctattg gcaccgttac
    19561 gtgaaacggt aaacaaaagc tatctggagc ctgcgcaaaa actggttgat tcactttctt
    19621 ctcgtaagaa tgccaccgag aagcaaatag cgactcttaa tgatcagctt gaaaaagcca
    19681 aaaaggatgg aaataccgag caaatccaga aactgcaagg cagtatccgt ggttatcagg
    19741 agcatttgga agccgttgct caggagctga ctcaggcaga gttcgagaga gataacgcgg
    19801 ccaaaactgg taagggcgta atgtcaaacc aggggactgt tcttgggtta ggtacaactg
    19861 ataaagctgc tcagaaggcg ctggcgcaat atatgcgaaa ccagatggat tctgcgactt
    19921 atcaacgtac tttgcctgac ggcactccga tgatggactt cgaaggtaag ccgattattg
    19981 ggcctaaaca actcaagacg cagcttaatt tgcagaaagc atccagcgcc agctctctgg
    20041 aaaaaatgag cgatgaagag cgtgccgcag ccattgctgc actgactaaa gctcgtgaac
    20101 aggatgcagc agccgccgag aaagcagccc aacgatcagc taacgcctcg cagcgtgcgg
    20161 ccaagaagga acaggcagcg caacagaaac tggcagccgg ataccagaag gctctggaca
    20221 aagccgatca gcttatgggg caaatgggtg aaagctctaa ggctacggta tcgtttgatc
    20281 agtctcttcg cgatacaacg aaatcgctga cagatttggc taatgcggtt cctaacgagt
    20341 tcatcactca agagatgatc gacaaggcga aaaaacgtct tgaagacctc aaaaatgcga
    20401 cacctgaata tcgcgagatg tttaatcgcc gcaatgttga gcagatgatc tccacttggg
    20461 caccggaatc agattccatt atcagtgctg gctatacgcc gtctcgtgaa gagaaagttg
    20521 ctgatttcga agacacctac aaccgcaatc ttaaagcgtt gatagaactt cgtgataagg
    20581 cgtctgatcc taaagtcgtg gcgctttata caaagaaaat caatcaactt attgctgctg
    20641 gcaataccgc gctcattaaa gagacaggga ctgcgacgca gaagttggca ctggaatacg
    20701 aaaacctggc agagcagatc gaaagcacct ggactgattt gtttagtggc ttaactgatg
    20761 tcctgactga tttcgttatt aacgggaaga tgagcttctc cagcctatct cagtccattt
    20821 tgaaagatat caccaatatg gtcgtgaagt cgcaaatcac gctgcctcta atgaacatgt
    20881 tggggatggg aaccaccgca gctggtagtt cacagagtgg caatttgctc actggagttg
    20941 cttctgccgt tgccaatcaa ggtgtacgaa tgggcaacac tgttaatggc gacaagtcgg
    21001 taggagaagc cacgaaggag acgtccagtt cggtaactgg attggggcaa acaacacagc
    21061 agaccaccag cgcaattggc acagcaacaa atgcgattgg tagctgggta tcggggctat
    21121 ttgatagcac cgaagccaaa gatgctgaga caaaagccgt gaaggactcc atcttctcga
    21181 tgcagaacct cagctctgtt accggcgctc tgtctgccgc gtttgcaatg cttggagcta
    21241 atgcttccgg ctctggtaat aagtggttga atttcggcgc gacagttgca tctggtttgg
    21301 tttcagtatg ggctggtggt ggattcgatg gattgatgtc cggttcttct ggctcaactt
    21361 ccgggttcaa caatctgacc agttctgctg ccgatggcac gaatggtatt cctgcaattc
    21421 cgaagtttgc aaatggtggc atattcggaa aagacggcgt gatcccactc cgggcatacc
    21481 agaaaggcgg tatcgccaac tcacctcaat tggcgttgtt tggggaaggt tctatgaatg
    21541 aggcgtatgt tccattgcct gatggtcgaa caatccctgt aacgctcagt accgatggta
    21601 tgagtggagg aggaaatgtt ctttctccgg tatcaattga gatcaacgtc catagtgacg
    21661 gtagcacaac tgaatccggc gatacagaaa gcatatggaa caatgccgct caacggatga
    21721 aagcaatcgc gcttgagact atcgctcaag agaaacgccc tggcggatca ctcaacccaa
    21781 acactcaacg taactaacta tcgactgccc cgaccggggc agtctcacaa ggatgtgaga
    21841 tggaaagaca aacgtttaat tggtatccag attacgaatc tgaaaaaagc gtaaaaccga
    21901 atgtaacggt acttaatttt ggtgatgact acgagcagcg acaggctcaa ggtcttaatc
    21961 gtattaaaga agaatggtcg ttaaccttta ccagatcata caacgaaatt aatgcaatcg
    22021 atgacttcct gactgagcga tcaggtgttg aatcgttcta ttgggttaat ccaagaggca
    22081 agcagattgt agttgtatgt gacagtcata cggtcaagcg atatcagggg tactgtgtct
    22141 taactgctac attcagacaa gtatttgagg cttaggcatc tggataagta agtactaatt
    22201 tactatcatt gtggcgctga caggatgtta gcgcctactt atttcaagga tgaaacaatg
    22261 ggaattaaag ctgatattca gagcttatct ccctctgcac tcattgagtt gttcgtactg
    22321 gatatgtcga acacaacttc aggggggaag ctattcttcc acgccggaac aaacgaactg
    22381 atgcaaccgg tcgtctggca aggagtgaca tacgagccgt ggccaatcaa agcatcaggc
    22441 tttgacaaaa ctggccaggg aacgttgcca cgtccaaaaa ttcaggtatc gaactttgcc
    22501 ggaaccgtct ctgcggaagt gcaggcaaac gacgatcttg ttggctgccg cattattcgc
    22561 aagatgacgc tggctcgctt cctcgatgcc gttaatttta aagacggcaa cccaacagca
    22621 gatccaaacc aacatttccc ggatgaaatg tggttcatcg aacagaaaac tctcgaaact
    22681 catcaggttg tcgagtttga attgtctagt gtgttcgatt tgatgggggt gcaactgccg
    22741 tatcgtcaga tcattaaaaa cacctgcccg tggaaatacc gagggccaga atgcggctat
    22801 accggtccat atttcgacaa aaataaccag cagacgtcta tgtctggtgc ggattactgc
    22861 acaaaacgtt atgacgcctg taatgcgcgt cggaattatt ttgccgacgg tgtgatccat
    22921 tttggcggat ttattggagc tacgcggtat gggtaataaa gcaatccctg agcttggctc
    22981 tgacgttatg cagcaaatct atctctgcgc cataaatcgc taccctaatg aagcgtgtgg
    23041 ctttctggtt agaactaatg gcgacaaata tcgctttatg gaagcgcgga atgtttcgga
    23101 gaacccgcag aacacttttg taatgcacgt tgacgacatt atggcggcag aggatgcggg
    23161 tgatgttatc gcaatctggc attcacatac tgatgaatca gcagaagcat ctgatgccga
    23221 tcgtgcaggc tgcgaagcga cggaagttcc gtggatgatt ctggctattc gcaagaatgt
    23281 tgagggagat gcccctttcc attttagcga gatgaatgtg atcacacctg atggtttcga
    23341 aatgccatac ctgggcagac cgtatgtatt tggcgtattc gattgctgga tgttgtgtcg
    23401 ggactatttg aagcgtgagt ttaacgtcga actaaacccg aacgcacacc tgcatattcc
    23461 atcgtggtac accggcgata acgacattct cgaccagaac taccgaaacg aaggattggt
    23521 acggcttgcg cccggaacag aacctcaacg tggggacgtc ttctttatcc aatacgggaa
    23581 aatgcctgac cactgcgcgg tttatatcgg cgatggaatg attcttcatc accagatcga
    23641 ccgccttagt tgtcgtgctt attacggcgg aatgtatcag aaacatacga cgcatcactt
    23701 gcgtcacaga gacttgctca agggagatga gacgtgtctg agttagttca tgtgcagctt
    23761 ggcggcccta tggccaaaca ttttggccgc cactggcatc taaaggtgcg caatacaaaa
    23821 caggctctgg atttaattga ggccaacaag cctgggttta aagcatggat gaagcgcaat
    23881 atcaaaacct atgaccgtta ccacatccag atcaccaata aacagggcca caagtggtct
    23941 gttgacgaaa gtgaatatca gatgatgggg cagtctgaca acattgccaa aatccgcatt
    24001 acacctgttc cgcgaggaag cggtggatct gcttttgggt ggtttcagac ggtagtaggg
    24061 gccgctttgt tggttgtatc ggcggtagtg atgcctgctt tagcacctct cggtttgtca
    24121 ctgatgatgg gcggcatatc acaaatcata tcgccgcaag ccactaacga aagtgtgaga
    24181 caggcagata actcgaactc ttattatttc gacggccctc aaaatacaga aaaccagggc
    24241 aacccagtac aacttatcta tggcgaggaa attctggttg gctcacaggt tgtgagttct
    24301 tcaatcacga ttgaccagct aatgtaaaca aggatttttt gaacatggaa cagttcaaga
    24361 agaaaaagct accgctgttt attgccggtg cgggtggtaa aaaaagcagc aaaagctcca
    24421 gccgtacacc ggttgaagcc gacgataccg taaattctcg tgcaatggcc gctatcctcg
    24481 atcttcttgg ggaaggggta attggcggct tagtaaatgg cgcaagatct atttttatcg
    24541 acgatctgcc gattgtaaat gaagacggtt cctccaactt cagcggaatc acatgggatt
    24601 ttcgcgacgg ttcacaagac cagactccaa tgtctggctt tgatttcgtt gaaacgccta
    24661 aatccgtcaa tatccagtta aaaaaaacac attatgtaac ggtttcaatc gataacgatg
    24721 aagctgatcg ggttcgtgtc atcatgaagt ttccttcttt gcgaagcata gataaaaaaa
    24781 ctggtgacac gaacggtacg actgttgagt acaagttcca gatagcaaat ggcgactcaa
    24841 catttgtaga tgtggtcgct gaaggtgaaa aaagcgttgg cattaagcta acagcaaaga
    24901 agaccggcgt ttattaccgt agctatgagc tgaagctgcc taagcctgga cgagcataca
    24961 aggttcgtgt ggtccgtatt accgatgata acagtagcca gtatctctat aacgatacat
    25021 gggtggattc aatcggggaa attgtcgata caccgatgaa ctatccaaac tctgttttgg
    25081 ttggattgaa ggtaaactct gagcaattcg gcagcaccat gccatctcgc tcttatctgg
    25141 tgcgtggcct aaaaattcga gtaccttcaa actataacga gtccagtaac acttacgctg
    25201 gggtatggga tggcactttt aagctgttgt cttcttcgaa ccccgcgtgg attctctttg
    25261 atttgctgac caatgctcga tatggccttg gtcagtatgt gtcggaatct atgattgacc
    25321 ttggccagtt gtaccagatt ggtcgatatt gtgacgaaga ggtcgatgat ggctttggtg
    25381 gcaaagagaa acgctttgca atcaatacgc agatcaccag tcgacaggac gcataccggc
    25441 taattcagga tattgctgga gcattccgcg gcatggtgtt ttgggctggt gggatggtta
    25501 acatcatgca ggatagccca tcagatccgg taatgatgtt taccaactct aacgtcaaag
    25561 atggattgtt tacctataaa ggttctgcgc gtaaagatcg cccatccgtt gcgctcgtaa
    25621 cctacaacaa caaggaggac ggttataagc aaaacatcga gtacgtcgaa gatcaggacg
    25681 caatgcgccg ttatggtgag cgtaaaacag aagtcgtagc atttggctgt acaagccgag
    25741 ggcaggctca ccgagttggt ttgtggcttt tatataccgc cagaatggag tcggatgtaa
    25801 ttacatttac tgccggctta gacgcgtcat ttctgatgcc tggtgaaacc gttctgattc
    25861 agaacaaata tcgcgcaggc aaacgtaact caggtcgaat tgtggcgttt accaaaaaca
    25921 gcgtcacact cgatgcgcct gtgtcgttag ccaaaggcgg ctgctttatt cgaatactga
    25981 atcaggaagg caaaatcgtt gaacgcgatg ttcttgaaac tggcgaaaat ataacaaaag
    26041 ttacgttttc aaaagccctg tcttcggctg aaacgcctgt tttgaacggc gtctggacta
    26101 ttacagaacc agacctcgaa cctatgcgcg ttcgcatcgt taacatcgcg cagggggaaa
    26161 cgccgggtag ctttgacatc accgctgttg agaacaatcc gtctaaatat gaggcaatcg
    26221 acaatggtgc aacgctgatc ccgcagaata cgacggtatt ggacccaact tactccaagc
    26281 catctaatct gcaaatcact gaagggactt atctctcaag cccgggcaac ctgtcagtaa
    26341 aactgactgc aacatgggaa gggaaatctc cagagtattg gatcagttgg cgacgttctg
    26401 atgaaaacaa tgtatcgaac tggcaatcgg cgcgtgtaac cgaagagcaa tacgaaatcg
    26461 ttaatgtcgc ggagaatgga cgctacgact tccagctgta tgcggtttca ttcaacggta
    26521 aaaaaacaga gattatcagt gccgtttatc aggtgttggg cacaatgaca ccgccggatt
    26581 ccccaacgtc attaacggcc gttggagact atcgtaatgt aatactgaat tgggttaatc
    26641 cagattcggt agaccttgat cacattaatg tttacgcatc ccagaccaac aatctggata
    26701 cggcgaaact gatcgcagag tctgcaagca ccacctttac acatgctggt cttggggata
    26761 gcgaaacgtg gtattactgg gttcgagcat cgaacaagcg aggtatgtta agtcctccga
    26821 actcaaactt gggtacagag gcgacaactc gcgatgtact gtcgttcctg actggcaaaa
    26881 ttacatcttc cgagcttggt caggctctgc ttgaggacat caacagcaaa gcctctcaag
    26941 aagcagttga cgagttaaat gagcatatca accagagcgt cgaatctttg gagggggagg
    27001 taaacgacgt taaggaagat atagctgaat tagataagca gttcagcgat aacctcgcgg
    27061 attttgaaat aaaattcaac gagcgtagcg atgctttgga gaacgcacaa accgagctta
    27121 aaggtgaggt ttcggcaacg atcgacaagg tcaatgaagc gtttgaaaaa attgatgcta
    27181 ctgatgctgc aattgttgaa atcgaaaaca ccgtatctga acacgataaa gctctcgcta
    27241 atacagtcga ggcaataaag gctgcaagag atgaagcggc ggctcttatt gctaaggaaa
    27301 gcgaggctcg tgttgaaggc gatgctgcaa acgctaaaca actggaagtg ttgcagtcaa
    27361 cggtagagga aagctccgct gccgttgaag aaatgaaaaa gacggttgca gaggtcgatc
    27421 gtgcaagtgc agaactgact acgaacattg aggcgttagc caaaacaaat attgacctgg
    27481 ctcttcgtca agatgaagac cagcacaagc agatggtcaa taacgcgaag atcgcaacaa
    27541 cacagaaaac ctttgctgat gatatgtctg caatggccac gaaggttgag gaaattcgcg
    27601 tagaaattgg cgaagacatt aaggccagta ttctggaaga gtccaccgct cgtgccgatg
    27661 gcgatgaagc gttggcaaag cgtgtcactc agctgcaatc taagtttgaa ggagatatca
    27721 gcgcggcgat tagtacagag caggaggctc gtacatctgc cgatgaagct ctgacgacac
    27781 agatcactca gcttgagtcg aaagtaagca acgatatcgt cgcagctatt aaggaagagc
    27841 aagaagcccg agcaacggaa gattcggctc tggcaagcca gatcacacaa cttcaggcaa
    27901 aggttgatga tgatatttct gctgcaatac gtagtgagca ggaggcccga gcaagcgggg
    27961 actctgcgtt agccaaacaa ataaaccagc ttcagtcaaa ggttgacggt gatatatccg
    28021 ctgccatcac tcaggagcag gaggccaggg caagtgctga ccaggcgctc tcacgagaaa
    28081 ttaacagcct gcgagcacaa accggaactg atattgccgc agcggttgct gtagagacaa
    28141 aagcaaggac tgatgccgat agtgcattgt ctacccagat tacgtctctt acagcaaaag
    28201 ctaatgatct cgaggcgtct cttgccagag aaacaacggc tcgtgcagat ggtgataccg
    28261 ctttaacaaa agaggtttca agtttaaaag cacagacagc gaaagatatt agtgcggccg
    28321 ttgcagttga aacgcaggct cgaactgatg ctgactcggc gttgtcgtct cagatcacca
    28381 agctgacctc tcaatacaaa gaggatatta aagcggctgt agcaaccgaa acgaaaacgc
    28441 gtacagagca agatgctgca ttggcgactc aaatcacaaa cttagagtct caaacggcag
    28501 ccaacatttc cgcggcggta acaactgaaa cgacagcgag aacgcaggca gataatgctc
    28561 tgagtgggcg aatcgatact ctgaaagcag aggtggatgg aaataccgcg acaattcaac
    28621 agcaggcaac agctattgct gataccaaca aaaaagtatc aaccgcgtgg acgttgaaaa
    28681 tggagacttc aacgagcgga gggcagaagt atgttgccgg tattgcgctt ggtattgaca
    28741 ccactgggct atctcagttt ttggttcagg cggacaggtt tggtttggtc aactctgtta
    28801 acgggaagat cacaacgcca tttgttatcg aaaacagtat cgcctatatg aatggagcgt
    28861 atatcaagga tggcacaatc accaatgcaa aagtaggcga tctgcaatct accaattttg
    28921 ttagcggtag atctggatgg cggttcggca aaaatggaac gcttgagatc aacggtaata
    28981 gcggcggcaa tgggcgattg gttataaatg gtcagcggat tgacgtttat gacgataaca
    29041 acgtcctacg agtaagaatt ggccgtctgt gatggtggaa aaaaattttt atat
//
LOCUS       DAZGVQ010000055        28895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000055 DAZGVQ010000000
VERSION     DAZGVQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.055"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(62..1726)
                     /gene="glnS"
                     /locus_tag="ACHLAG_002269"
     CDS             complement(62..1726)
                     /gene="glnS"
                     /locus_tag="ACHLAG_002269"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFP0655493.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1929..3875)
                     /gene="nagE"
                     /locus_tag="ACHLAG_002270"
     CDS             complement(1929..3875)
                     /gene="nagE"
                     /locus_tag="ACHLAG_002270"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFP0655494.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4208..5008
                     /gene="nagB"
                     /locus_tag="ACHLAG_002271"
     CDS             4208..5008
                     /gene="nagB"
                     /locus_tag="ACHLAG_002271"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP0655495.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5068..6216
                     /gene="nagA"
                     /locus_tag="ACHLAG_002272"
     CDS             5068..6216
                     /gene="nagA"
                     /locus_tag="ACHLAG_002272"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP0655496.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6225..7445
                     /gene="nagC"
                     /locus_tag="ACHLAG_002273"
                     /gene_synonym="nagR"
     CDS             6225..7445
                     /gene="nagC"
                     /locus_tag="ACHLAG_002273"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFP0655497.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7493..8245
                     /gene="nagD"
                     /locus_tag="ACHLAG_002274"
     CDS             7493..8245
                     /gene="nagD"
                     /locus_tag="ACHLAG_002274"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFP0655498.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8502..10166
                     /gene="asnB"
                     /locus_tag="ACHLAG_002275"
     CDS             8502..10166
                     /gene="asnB"
                     /locus_tag="ACHLAG_002275"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFP0655499.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLL
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10547..10623
                     /locus_tag="ACHLAG_002276"
     tRNA            10547..10623
                     /locus_tag="ACHLAG_002276"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10581..10583,aa:Met,seq:cat)
     gene            10632..10716
                     /locus_tag="ACHLAG_002277"
     tRNA            10632..10716
                     /locus_tag="ACHLAG_002277"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10666..10668,aa:Leu,seq:tag)
     gene            10740..10814
                     /locus_tag="ACHLAG_002278"
     tRNA            10740..10814
                     /locus_tag="ACHLAG_002278"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10772..10774,aa:Gln,seq:ttg)
     gene            10849..10923
                     /locus_tag="ACHLAG_002279"
     tRNA            10849..10923
                     /locus_tag="ACHLAG_002279"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10881..10883,aa:Gln,seq:ttg)
     gene            10939..11015
                     /locus_tag="ACHLAG_002280"
     tRNA            10939..11015
                     /locus_tag="ACHLAG_002280"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10973..10975,aa:Met,seq:cat)
     gene            11064..11138
                     /locus_tag="ACHLAG_002281"
     tRNA            11064..11138
                     /locus_tag="ACHLAG_002281"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11096..11098,aa:Gln,seq:ctg)
     gene            complement(11292..12467)
                     /gene="ubiF"
                     /locus_tag="ACHLAG_002282"
     CDS             complement(11292..12467)
                     /gene="ubiF"
                     /locus_tag="ACHLAG_002282"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFP0655500.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12613..14037
                     /gene="miaB"
                     /locus_tag="ACHLAG_002283"
     CDS             12613..14037
                     /gene="miaB"
                     /locus_tag="ACHLAG_002283"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFP0655501.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14190..15230
                     /locus_tag="ACHLAG_002284"
     CDS             14190..15230
                     /locus_tag="ACHLAG_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFP0655502.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15227..15694
                     /gene="ybeY"
                     /locus_tag="ACHLAG_002285"
     CDS             15227..15694
                     /gene="ybeY"
                     /locus_tag="ACHLAG_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFP0655503.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15784..16662
                     /gene="corC"
                     /locus_tag="ACHLAG_002286"
     CDS             15784..16662
                     /gene="corC"
                     /locus_tag="ACHLAG_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFP0655504.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16687..18225
                     /gene="lnt"
                     /locus_tag="ACHLAG_002287"
     CDS             16687..18225
                     /gene="lnt"
                     /locus_tag="ACHLAG_002287"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFP0655505.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIVSLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLLGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREALTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(18303..20180)
                     /locus_tag="ACHLAG_002288"
     CDS             complement(18303..20180)
                     /locus_tag="ACHLAG_002288"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFP0655506.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLMTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSNKPCAWV
                     MALSPDEKILLIPGMVRGDIVRINTITHQKESYPASDARGTISAMRFRPENGDVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            20635..21543
                     /gene="gltI"
                     /locus_tag="ACHLAG_002289"
     CDS             20635..21543
                     /gene="gltI"
                     /locus_tag="ACHLAG_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFP0655507.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            21713..22453
                     /gene="gltJ"
                     /locus_tag="ACHLAG_002290"
     CDS             21713..22453
                     /gene="gltJ"
                     /locus_tag="ACHLAG_002290"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFP0655508.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            22453..23127
                     /gene="gltK"
                     /locus_tag="ACHLAG_002291"
     CDS             22453..23127
                     /gene="gltK"
                     /locus_tag="ACHLAG_002291"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFP0655509.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            23127..23852
                     /gene="gltL"
                     /locus_tag="ACHLAG_002292"
     CDS             23127..23852
                     /gene="gltL"
                     /locus_tag="ACHLAG_002292"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFP0655510.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            23970..24905
                     /gene="rihA"
                     /locus_tag="ACHLAG_002293"
     CDS             23970..24905
                     /gene="rihA"
                     /locus_tag="ACHLAG_002293"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFP0655511.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQKC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            24989..26659
                     /gene="hscC"
                     /locus_tag="ACHLAG_002294"
     CDS             24989..26659
                     /gene="hscC"
                     /locus_tag="ACHLAG_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFP0655512.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGTNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(26719..28170)
                     /gene="djlC"
                     /locus_tag="ACHLAG_002295"
     CDS             complement(26719..28170)
                     /gene="djlC"
                     /locus_tag="ACHLAG_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFP0655513.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(28167..28874)
                     /locus_tag="ACHLAG_002296"
     CDS             complement(28167..28874)
                     /locus_tag="ACHLAG_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP0655514.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
BASE COUNT         7110 a         7505 c         7285 g         6995 t
ORIGIN      
        1 tgtttgctat ttcttgccgg atgcgacgta aatgcctcac ccggcctggt agaacttaaa
       61 attactcgcc tactttcgcc caggtatcac gcagcccaac ggtgcggtta aataccggtt
      121 tttccgctgt ggaatggcgg ctgtcgaggc agaagtaacc ttcacgctca aactggaatg
      181 ctttacccgc aaccgcatct ttcagcgacg gttcagcaaa gccctgtttg atcaccagcg
      241 attccgggtt aatcaccgac aggaaatcat ccgcagcacc agggttcggc acgctgaaca
      301 gacggtcgta cagacggatt tcaaccggca gcgcatgtgc cgcgctcacc cagtgaataa
      361 cacctttgac tttacgacca tctgccggat ctttgcttaa ggtgtcggcg tcataagtac
      421 agaagatggt ggtgatattg ccttcggcat ctttctccac gcgttctgcc ttaatgacgt
      481 aagcattacg cagacgcact tctttaccca gcaccagacg tttgtactgc ttgttagctt
      541 cttcgcggaa atcggcgcga tcgatccaaa tctcaccgct aaacggcacc tggcggctac
      601 ccatttccgg tttgttcgga tggttcggca tggtaaccat ttcgccttcg ccctgatagt
      661 tttcgataac cagtttcacc ggatcgataa ccgccattgc gcgcggcgca ttttcgttga
      721 gatcttcacg gatgcaggat tccagcgatg ccatctcaat ggtgttgtcc tgcttggtca
      781 cgccgatgcg tttgcagaac tcacgaatag aagcagcagt gtaaccacga cgacgcagac
      841 cggaaatggt cggcatacgc gggtcatccc agccttcaac gtgcttgtcg gtcaccagca
      901 ggttcagctt acgcttggac atcacagtgt attccagatt caggcgcgag aattcgtact
      961 ggcgcgggtg aacaggaatg gtgatgttgt ccagcaccca gtcgtacagg cgacggttgt
     1021 cctggaactc aagcgtacac agagagtgcg taataccttc cagggcatcg ctgatgcagt
     1081 gggtgaagtc gtacatcggg tagatgcacc acttgttgcc agtctggtgg tgttcagcaa
     1141 atttaatacg gtacagcacc ggatcgcgca tcacgataaa cggtgaagcc atgtcgattt
     1201 tcgcacgcag gcaggcttta ccttcttcaa aaccaccggc acgcattttt tcgaacagcg
     1261 ccaggttctc ttcaacgctg cggtcgcggt acgggctgtt tttacccggt tgcgtcaggg
     1321 tgccgcggta ttcgcggatc tgttccggcg tcagttcatc aacgtacgcc aggcctttat
     1381 tgatcagttc gatcgcatag gcgtggagct gatcaaaata atcggaggag taacggacgt
     1441 taccagacca gtgaaaacct aaccactcta cgtcgttttt gatcgactca acatactcga
     1501 tatcttcttt taccgggtta gtgtcgtcga aacgcaggtt gcactggcct ttatagtcct
     1561 gggcgatccc gaagttcagg cagatagatt tcgcatggcc aatatgcaga tagccattcg
     1621 gctccggcgg gaaacgggtg tgtactgtgg tgtgcttacc actggccaga tcttcatcga
     1681 tgatctgacg gataaagtta gtcgggcggg cttctgcctc actcatcgtg gattcctcaa
     1741 agcgtaaaca acgtataacg gcgtatgatc ttataagtgg gacaggctga caactgttag
     1801 ttttttaaaa ttgataatct aacaaggatt atggggatga ttgatgaaaa aaaaagcggc
     1861 agagaagatc tccaccgctt gtcattgttg gatgcgacgc tcaagcgtcg catcaggcat
     1921 aaagcagatt actttttgat ttcatacagc ggtgtttgac ccgccacaac atggccctga
     1981 gctttaatga tcaagccact gaaatcgtcg atattgctgc aaaccaccgg gctaatcatc
     2041 gagcgggcgt tagcgttcag gtaatccaga tccatttcca gaatcggttg ccctgcgctt
     2101 acctgcgcac cctcttccac cagacgttta aagcctttac cttccagcgc tacggtgtcg
     2161 atacccatat ggacgacgat ctccgcgcct ttttcggttt ccaggcagaa cgcgtggttg
     2221 gtgttgaaga ttttcacgat tgtccctgcg gctggtgata cgacgatttt atctgtcggt
     2281 ttcaccgcca caccgtcacc caccgctttg ctggcgaatg cttcgtcagg aacctgatcc
     2341 agtgccacga catcaccggt aatcggcgat accagctccg cgatagatac cgcgtttggt
     2401 acagcctgcg gttttgctac aggcgcggca gttgccggag ttgcttcagc tgacgcagcg
     2461 gctaccggac cacgggcaac gactttcttc atcgcatcgc cgatggattc tgctttcgcg
     2521 ccaacaatca cctgaatagt ctgtttgttc agtttcacta caccagaagc acccagacgt
     2581 ttacacatcg tatcgttgac gcgggcagag tcagccacgg tcaggcgcag acgggtgata
     2641 caggcgtcaa ttgctttcag gttgtcagtg ccgccaaccg cagcaatata gttggtcgcc
     2701 agttgattga ggccttcttc agtgttgctg ttggcttctt cagtaacgat ctcgtcttct
     2761 ttatcttcac gacccggcgt tttcaggttg aacatgcgga taaccaaact gaacaccacg
     2821 aagtagatag cgaagaagac aacacccata accagcagca tccagacgtt ctggctggcg
     2881 gccggcaggt tatacatcaa cgcgtagtcg atagcccccg cagagaaaga gaagccagca
     2941 tggataccca gcagtgttgc cacaaacagg ctgataccgg tcagcagtgc gtgcaggagg
     3001 tacagcagcg gtgcaaggaa catgaacagg aattccagcg gctcagtcac accggtcagg
     3061 aacgcagtaa cagcaacaga aagcagcatc ccgccaacca tcggacgacg ctctttcggt
     3121 gctgcgaagt acatcgccag cgccgcaccc ggcagaccga acatcataat cgggaagaag
     3181 ccggacatga acatccccgc ggtgccgtca ccggcataga agcggttgat atcaccgtgg
     3241 aaaaccgtac ccgccgcgtt ggtgaattca ccaatctgga accaggcgat ggtgttcagc
     3301 acctgatgca gaccggttgg gatcagcaga cggttgatga aaccaaagat accggaaccc
     3361 agcgcgcccg cagaaacgat ccactcgcct cctgcatgga tagcgtgctg taccggcggc
     3421 cagacgtaac caaaaatggc cgccagcacc aggcagaaga agccggtggc aatcggcaca
     3481 aagcgtttgc cgccgaagaa actcaggaag tccggcagtt taatatcgga ccaacggtta
     3541 taggctgcgc caccaaccag accggtaatg atacccgcca gtacacccat gttaatttct
     3601 gggttgatgg tcaccatcgc tttggttaac acaaagtaac ctaccgcacc cgccagcgcc
     3661 gccgcacctg cgctgtcttt cgaccagctg gatgccacac cgatggcgaa gattaatgcg
     3721 aggttatcaa aaatcgcacc gcccgcctgg gcaataaacg caacgttaag taaatctggc
     3781 tgaccgaatc gcagcaacag tgccgccacc ggcagcaccg cgatagggag ctgtaacgcc
     3841 ctaccgagtc gctggaaaaa acctaaaata ttcatcttat tccccctacg agaaccctat
     3901 ttggctcgtt taaagccgta tttttatttt gctgcaaatt gtactgccga tgttctgtaa
     3961 tcagattgtt agatcatctg ctacagagtg tgtgaaaatt taattcgtat cgcaaattaa
     4021 acgcgtgtct tttgtgagtt ttgtcaccaa atatcgttat catcactccc ttttactggc
     4081 taaagcagaa aacttatttt atcattcaaa aaatcaggtc ggattgacgc ctgtctgcgc
     4141 aaatccaggt tacgcttaaa gatgcctaat ccgccaacgg cttacatttt acttattgag
     4201 gtgaataatg agactgatcc ccctgactac cgctgaacag gtcggcaaat gggctgctcg
     4261 ccatatcgtc aatcgtatca atgcgttcaa accgactgcc gatcgtccgt ttgttctggg
     4321 cctgccgact ggcggcacgc cgatgaccac ctataaagcg ttagtcgaaa tgcataaagc
     4381 aggccaggtc agctttaagc acgttgtcac cttcaacatg gacgaatatg tcggtctgcc
     4441 gaaagagcat ccggaaagct actacagctt tatgcaccgt aatttcttcg atcacgttga
     4501 tattccagca gaaaacatca accttctcaa cggcaacgcc ccggatatcg acgccgaatg
     4561 ccgccagtat gaagaaaaaa tccgttctta cggaaaaatt catctgttta tgggcggtgt
     4621 aggtaacgac ggtcatattg catttaacga accggcgtct tctctggctt ctcgtactcg
     4681 tatcaaaacc ttgactcatg acactcgcgt cgcgaactct cgtttctttg ataacgatgt
     4741 caatcaggtg ccaaaatatg ccctgactgt cggtgttggt acattgctgg atgctgaaga
     4801 agtgatgatt ctggtgctgg gtagccagaa agcactggca ctgcaggctg cagttgaagg
     4861 ttgcgtgaac cacatgtgga ccatcagctg tctgcaactg catccaaaag cgatcatggt
     4921 gtgcgatgaa ccttccacca tggagctgaa agttaagact ttaagatatt tcaatgaatt
     4981 agaagcagaa aatatcaaag gtctgtaatt gttatccctg ccctggctcc ttgctcaggg
     5041 caatattttt taaaatcggg ggtcagaatg tatgcattaa cccagggccg gatctttacc
     5101 ggccacgaat ttcttgatga ccacgcggtt gttatcgctg atggcctgat taaaagcgtc
     5161 tgtccggtag cggaactgcc gccagagatc gaacaacgtt cactgaacgg ggccattctc
     5221 tcccccggtt ttatcgatgt gcagttaaac ggctgcggcg gcgtacagtt taacgacacc
     5281 gctgaagcgg tcagcgtgga aacgctggaa atcatgcaga aagccaatga gaaatcaggc
     5341 tgtactaact atctgccgac gcttatcacc accagcgatg agctgatgaa acagggcgtg
     5401 cgcgttatgc gcgagtacct ggcaaaacat ccgaatcagg cgttaggtct gcatctggaa
     5461 ggtccgtggc tgaatctggt aaaaaaaggc acccataatc cgaattttgt gcgtaagcct
     5521 gatgccgcgc tggtcgattt cctgtgtgaa aacgccgacg tcattaccaa agtgaccctg
     5581 gcaccggaaa tggttcctgc ggaagtcatc agcaaactgg caaatgccgg gattgtggtt
     5641 tctgccggtc actccaacgc gacgttgaaa gaagcaaaag ccggtttccg cgcggggatt
     5701 acctttgcca cccatctgta caacgcgatg ccgtatatta ccggtcgtga accgggcctg
     5761 gcgggcgcga tcctcgacga agctgacatt tattgcggta ttattgctga tggcctgcat
     5821 gttgattacg ccaacattcg taacgctaaa cgtctgaaag gcgacaaact gtgtctggtt
     5881 accgacgcca ccgcgccagc aggtgccaac attgaacagt tcatttttgc gggtaaaaca
     5941 atatactacc gtaacggact ttgtgtggat gagaacggta cgttaagcgg ttcatcctta
     6001 accatgattg aaggcgtgcg taatctggtc gaacattgcg gtatcgcact ggatgaagtg
     6061 ctgcgtatgg cgacgctcta tccggcgcgt gcgattggcg ttgagaaacg tctcggcaca
     6121 ctcgccgcag gtaaagtagc caacctgacc gcattcacac ctgattttaa aatcaccaag
     6181 accatcgtta acggtaacga ggtcgtaact caataagaga aagtatgaca ccaggcggac
     6241 aagctcagat aggtaatgtt gatctcgtaa aacagcttaa cagcgcggcg gtttatcgcc
     6301 tgattgacca gtacgggcca atctcgcgga ttcagattgc cgagcaaagc cagcttgccc
     6361 ccgccagcgt aaccaaaatt acgcgtcagc ttatcgaacg cgggctgatc aaagaagttg
     6421 atcagcaggc ctccaccggg ggccgccgcg ctatctccat cgtcaccgaa acccgcaatt
     6481 tccacgcaat cggcgtacgg cttggtcgtc acgacgccac catcaccctg tttgatctca
     6541 gcagcaaagt gctggcagaa gaacattacc cgctgccgga acgtacccag caaacgctgg
     6601 aacatgccct gttgaatgcc attgctcagt ttattgatag ctaccagcgc aaactgcgcg
     6661 agctgatcgc gatttcggtg atcctgccag ggcttgttga cccggacagc ggcaaaattc
     6721 attacatgcc gcatattcag gtagaaaact gggggctggt agaagcactg gaagaacgtt
     6781 ttaaagtgac ctgtttcgtt ggtcacgata tccgtagtct ggcgctggcg gagcactact
     6841 tcggtgcaag tcaggattgc gaagactcca ttctggtgcg tgtccatcgc ggaaccgggg
     6901 ccgggattat ctctaacggg cgcattttta ttggccgcaa cggcaacgtc ggtgaaattg
     6961 gccatattca ggtcgaaccg ctgggtgaac gctgccactg cggcaacttt ggctgcctgg
     7021 aaactatcgc tgccaacgcc gctattgaac aacgggtgtt gaatctgtta aagcagggct
     7081 accagagccg cgtgccgctg gacgactgca ccatcaaaac tatctgcaaa gccgcgaaca
     7141 aaggcgatag cctggcctcg gaagtgattg agtatgtcgg tcgtcatctg ggcaaaacca
     7201 tcgccattgc tatcaactta tttaatccgc aaaaaattgt tattgccggt gaaatcaccg
     7261 aagccgataa agtgctgctc cctgctattg aaagctgcat taatacccag gcgctgaagg
     7321 cgtttcgcac taatctgccg gtggtacgtt ctgagctgga tcaccgctcg gcaatcggcg
     7381 cttttgcgct ggtaaaacgc gccatgctca acggtatttt gctccagcat ttgctggaaa
     7441 attaatgtgc ttttatagtg gcgcttattg ttgtcaatat tctgggtagt ccatgaccat
     7501 taaaaatgta atttgcgata tcgacggcgt gctgatgcac gataacgtcg ccgtaccggg
     7561 tgcagcggaa tttttgcacg ggattatgga taaaggcctg ccgctggtgt tgctgaccaa
     7621 ctatccttcg cagaccgggc aagatctggc gaaccgcttt gccactgccg gtgtcgatgt
     7681 gccggatagc gtgttttata cctctgcgat ggcgactgcc gatttcctgc gtcgccagga
     7741 aggcaagaaa gcgtatgtgg tgggcgaagg cgcactgatt catgaactgt acaaagccgg
     7801 tttcactatt accgatgtga accctgattt tgtgattgtt ggcgaaacgc gttcctacaa
     7861 ctgggacatg atgcataaag cggcctattt cgtcgctaac ggtgcacgct ttatcgccac
     7921 caacccggac acccacgggc gcggttttta tcccgcttgt ggcgcgttgt gtgcagggat
     7981 tgagaaaatc tccgggcgca aaccgttcta tgttggtaag cccagcccgt ggatcatccg
     8041 cgcggcatta aacaaaatgc aggcgcattc ggaagaaacg gtgattgtcg gcgataactt
     8101 gcgtaccgat attctggcgg gcttccaggc gggtctggag acgattctgg tgctttctgg
     8161 tgtttcatcg ctcgacgata tcgacagtat gccgttccgc ccaagctgga tttacccgtc
     8221 ggtcgctgaa atcgacgtta tctgaatagc aaccacgtct cagggcgtgg tttcttattt
     8281 ttacccatca ttaaaatcat cccattcaat aaaactcgcc aaaaattatc gatccatcaa
     8341 taaatacatc accacaccat cccctttttc acaaatcagc aatcaccatt acgtttttta
     8401 tttttgcact gccaaatcgc ttgcgcacgg tgctgctttg cggcattttt ttaaacaagc
     8461 aaacacaaca agcaacaaat accaggttaa cggagaaggt tatgtgttca atttttggcg
     8521 tattcgatat caaaacagac gcagttgagc tgcgtaagaa agcactcgag ctgtcacgcc
     8581 tgatgcgtca tcgtggcccg gactggtccg gtatttatgc cagcgataac gccattctcg
     8641 ctcacgaacg tctgtcaatt gttgacgtta acgcgggggc gcaacctctc tacaaccaac
     8701 aaaaaaccca cgtactggcg gtaaacggtg aaatctacaa ccaccaggca ttgcgcgctg
     8761 aatatggcga tcgttatcag ttccagacag ggtctgactg tgaagtgatc ctcgcgctgt
     8821 atcaggaaaa agggccggaa tttctcgacg acttgcaggg catgtttgcc tttgccctgt
     8881 acgacagcga aaaagatgcc tacctgattg gtcgcgacca tctggggatc atcccactgt
     8941 atatgggcta tgacgaacac ggtcagctgt atgtggcctc agaaatgaaa tcgctggtgc
     9001 cagtttgccg cacgattaaa gagttcccgg cggggagcta tttgtggagc caggacggcg
     9061 aaatccgttc ttattatcat cgcgactggt tcgactttga tgcagtgaaa gataacgtga
     9121 ccgacaaaaa cgagctgcgt caggctctgg aagattccgt taaaagccat ctgatgtctg
     9181 atgtgcctta cggtgtgctg ctttctggtg gtctggattc ctcaattatt tccgctatca
     9241 ccaagaaata cgcagcccgt cgcgtggaag atcaggaacg ctctgaagcc tggtggccgc
     9301 agttacactc ctttgctgta ggtctgctgg gttcaccgga tcttaaagca gcccaggaag
     9361 tggcaaacca tctgggcacg gtgcatcacg aaattcactt cactgtacag gaaggtctgg
     9421 atgccatccg cgacgtgatt taccacatcg aaacttatga tgtgaccact attcgcgctt
     9481 caacaccgat gtatttaatg tcgcgtaaga tcaaggcgat gggcattaaa atggtgctgt
     9541 ccggtgaagg ttctgatgaa gtgtttggcg gttatcttta cttccataaa gcgcccaacg
     9601 ctaaagaact gcatgaagag acggtgcgta aactgctggc cctgcatatg tatgactgcg
     9661 cgcgcgccaa caaagcgatg tccgcctggg gcgtggaagc gcgcgttccg ttcctcgaca
     9721 aaaaattcct tgatgtggcg atgcgtatta acccacagga taaaatgtgc ggtaacggca
     9781 aaatggaaaa acacatcctg cgtgaatgtt ttgagtcata cctgcctgca agcgtggcct
     9841 ggcggcagaa agagcagttc tccgatggcg tcggttacag ttggatcgac accctgaaag
     9901 aagtggcggc gcagcaggtt tctgatcaac agctggaaac tgcccgcttc cgcttcccgt
     9961 acaacacgcc gacgtccaaa gaagcgtatc tgtaccggga gatctttgaa gaactgttcc
    10021 cacttccgag cgccgctgag tgcgtaccgg gcggtccttc cgtcgcgtgt tcttccgcta
    10081 aagcgattga gtgggatgag gcgttcaaga aaatggacga tccgtctggt cgtgcggttg
    10141 gcgttcacca gtcggcgtat aagtaagaaa tgcgaaacag ccccggtgaa atccggggct
    10201 gtttcagtta ttgaggtgct tgttaattct gatatgcagt aaggaaatgt tccgggtttg
    10261 ccacatcact gtttgacaac tcatcactgt aggccagtat ttcatagatt aatttcccac
    10321 cactattgtt gaattccgaa tttcatcctg tacgacaaaa gaatggctgt agggtgggga
    10381 aaaaacacaa agtaaacaat aattgacgaa tatagcgcca cgctgttcgc aacctaacca
    10441 aacagtcact ttcgagcaat tttccttgaa aaagaggttg acgctgcaag gctctatacg
    10501 cataatgcgc cccgcaacgc cgataaggta tcgcgaaaaa aaagatggct acgtagctca
    10561 gttggttaga gcacatcact cataatgatg gggtcacagg ttcgaatccc gtcgtagcca
    10621 ccatcttttt tgcgggagtg gcgaaattgg tagacgcacc agatttaggt tctggcgccg
    10681 caaggtgtgc gagttcaagt ctcgcctccc gcaccattca ccagaaagcg ttgtacggat
    10741 ggggtatcgc caagcggtaa ggcaccggtt tttgataccg gcattccctg gttcgaatcc
    10801 aggtacccca gccatcttct tcgagtaagc ggttcaccgc ccggttattg gggtatcgcc
    10861 aagcggtaag gcaccggttt ttgataccgg cattccctgg ttcgaatcca ggtaccccag
    10921 ccatcgaaga aacaatctgg ctacgtagct cagttggtta gagcacatca ctcataatga
    10981 tggggtcaca ggttcgaatc ccgtcgtagc caccaaattc tgaatgtgtc gaatacgttc
    11041 ggcaaattca aaaaccaatt tgttggggta tcgccaagcg gtaaggcacc ggattctgat
    11101 tccggcattc cgaggttcga atcctcgtac cccagccaca ttaaaaaagc tcgcttcggc
    11161 gagctttttg cttttctgcg ttcattcaat gtcgaatgcg atgttgacac gtcttatcct
    11221 tcaatgtcgg atgcgacgct gccgcgtctt atccgaccta cggttggcac gcatccggca
    11281 atgttgtaag gctacaaccc taacgcatat ttcagcgcct gacgtttcaa cacgccagca
    11341 cgctctgccg ccattaaccc gagattacgc acaaaacgca gcggcggcag attattactg
    11401 aatccggcat aaaacagatc cataccactt tgcataatga agttatccgc catgcgccgc
    11461 atctggtaac gcttgaggac aggataactg gcccacgctt caccgtagct gcgggcattc
    11521 accagaacat caatcagggc atcgacatca cgataaccaa gattcacccc ctgccccgcc
    11581 agcggatgga tggtatgcgc ggcatcgccc accagcgcaa gccccggctg cacatactgc
    11641 aacgcatgtc ggcgcgtcag cggaaacgca ccagcggcaa gcggtgtaac gtaacccaga
    11701 cgcgacggga aatgcttcgc gatttccgcc tggagttgcg ccatattcat attctgcaac
    11761 tggcgaatac gtaccggaga gtcataccac accagcgatg cccagttatc aaacaacggc
    11821 agaaacgcac gtggtccgtc cggggtaaat tgctgccagg tgctgtcgcc gggatcgttc
    11881 tcacactgta cgctaatcaa catacacgac tgcgcatact gccaggcatg aacgccaatt
    11941 cccgccatct gccgcacctg cgaatttgcg ccgtcagcac caatcaccag cttcgcgcga
    12001 atcacttcac cgcccttcag ttccagctcc tgaagatcat tatctcgatg taatgcaatc
    12061 agcgagcctg gcacacgtaa cgttactttc ggatgcgctt ccagcgcctg ccacaatgcc
    12121 tgttgcagga cggtgttttc caccatatag ccaagcagcg gcagcttaag ttcagcggcg
    12181 tcaaacatca catgcgccgt ttcccactcc cacgtttcca gtctgcggta aggatggcaa
    12241 cgcatagcct gtactgcatc ccagacccct aaccctttaa gcaatgatac cgatgcggcg
    12301 ctgatcgccg agatccgcac gtccggttgg ctatcagcga caaacggtgc aggttctgcg
    12361 tgctcgatca ccgttaccga aaatccgtgc tgtgccagcc ccagcgccag tgcgccgccg
    12421 accattcctc cgccgacaat ggcaatttcc gttggttgat ttgtcatggt caatcatcct
    12481 gttagcaata tgctttaagt ttacaggatt tttaccccca agggctgata acgctcatac
    12541 tggtcacaac ggcagcaaag cattacacta tgcgccctgc attcctggct actatttcgc
    12601 aagagcaagt cgatgaccaa aaaactccat attaaaacct ggggctgtca gatgaacgag
    12661 tacgattcat cgaagatggc cgatctgctg gatgccaccc acggctatca actgaccgac
    12721 gtggcggaag aagcggatgt gctgctgctg aacacctgct caatccgcga gaaggctcag
    12781 gaaaaagtct tccatcagtt gggtcgctgg aaactgttaa aagagaagaa tccagacctg
    12841 attatcggcg tcggtggctg cgtggcatcg caagaaggcg agcacattcg ccagcgcgcc
    12901 cactatgtcg atattatttt tgggccgcaa acgctgcacc gtctgccgga gatgatcaac
    12961 tccgtgcgcg gcgaccgcag cccggttgta gatatcagct tcccggaaat cgagaagttt
    13021 gaccgtctac cggaaccgcg cgccgaaggg ccgaccgcgt ttgtctccat catggaaggc
    13081 tgcaataaat attgtaccta ctgcgtggtg ccttacaccc gtggtgaaga ggtaagccgt
    13141 ccgtctgacg atattctgtt tgagattgcc cagcttgctg ctcagggcgt gcgtgaagtc
    13201 aacctgctcg gtcagaacgt gaacgcctgg cgtggtgaaa actacgacgg caccaccgga
    13261 tcgtttgccg atctgctgcg tctggttgct gcgatcgacg ggatcgatcg tattcgcttt
    13321 accaccagcc atccgatcga attcaccgac gatatcatcg aagtgtaccg cgacacgcct
    13381 gagctggtga gtttcctgca tctgccggta cagagcggtt ccgatcgcat tctgaacctg
    13441 atgggccgta cccatacggc gctggagtac aaagcgatca tccgtaaact gcgtgcggcg
    13501 cgtccagata ttcagatcag ttctgacttc attgttggct tccctggcga aaccaccgaa
    13561 gacttcgaga aaacgatgaa gctgattgcc gacgtcaatt tcgacatgag ctacagcttt
    13621 atcttctctg cacgtccggg tacaccagcc gccgatatgg ttgatgatgt tccggaagaa
    13681 gagaagaagc aacgtctgta tattctgcaa gaacgtatca atcagcaagc gatggcatgg
    13741 agccgccgga tgctcggcac cacccagcgt attctggtag aagggacttc gcgtaagagc
    13801 atcatggagc tttccgggcg taccgaaaat aaccgcgtgg tcaacttcga gggcacaccg
    13861 gatatgatcg gtaaattcgt cgatgtagaa attaccgacg tctacccgaa ctctctgcgc
    13921 ggtaaagtgg tgcgtactga agatgaaatg ggtctgcgcg tggcagaaac accggaatcg
    13981 gtgattgccc gtacccgcaa agaaaacgac cttggcgtgg gttattatca gccgtaattc
    14041 tcaggcccgc cgttccggtg ggccttgttt tttctctctt tatccccatc tttatcgcaa
    14101 tgcttgcgcc tttttcctgt ggcgtgaata atttccttat aggccggtga atgttcagct
    14161 tccggcgaca tacagataag aggaacggtt tgaacataga cactcgcgaa atcaccctgg
    14221 agccagcaga caacgcgcgt ctgttgagcc tgtgcggccc gtttgatgac aacatcaagc
    14281 agctcgaacg ccgtctcggc atcgagatca atcgccgcga caaccacttt aaactgaccg
    14341 gccgtccgat ttgcgtcacc gctgcggcag acattctgcg tagcctgtat gtcgatacag
    14401 ccccgatgcg cggtcagatt caggatatcg aaccggaaca gatccacctt gcgattaaag
    14461 aagcgcgagt actggagcaa agtgcggaga gcgtgccgga gtacggcaaa gcggtcaata
    14521 tcaaaaccaa acgcggcgta attaagccgc gcacgccaaa ccaggcgcag tacatcgcca
    14581 atattctcga ccatgacatt accttcggcg ttggcccggc gggtacgggt aaaacctacc
    14641 tggcagtggc tgcggcagtt gatgccctgg agcgtcagga gattcgccgt attctgctga
    14701 ctcgtccggc ggtcgaagcc ggtgagaaac tgggcttcct gcctggcgat ttaagccaga
    14761 aagtagaccc gtatctgcgc ccgctgtacg acgcgctgtt tgaaatgctg ggctttgaga
    14821 aagttgagaa actgattgag cgcaacgtta ttgaagtcgc gccgctggcc tatatgcgtg
    14881 gtcgtacgct gaacgacgcg tttatcattc tcgatgagag ccagaacacc accatcgaac
    14941 agatgaagat gttcctgacc cgtatcggtt ttaactcaaa agcggttatc accggcgacg
    15001 tcacacagat cgacctgccg cgtaatacta aatcaggctt acgtcacgcc atcgaagtgc
    15061 tggccgatgt cgaagagatc agctttaact tcttccacag cgaagacgtg gttcgtcacc
    15121 ctgtggtggc gcgtatcgtt aacgcctatg aagcctggga agaagccgaa caaaaacgaa
    15181 aagcggcgct ggcggcagaa cgcaagcgcg aagaacagga acaaaaatga gtcaggtgat
    15241 cctcgattta caactggcat gtgaagataa ttccgggtta ccggaagaga gccagtttca
    15301 aacatggctg aatgcggtga tcccgcagtt tcaggaagaa tcggaagtga ctattcgcgt
    15361 ggtcgatacc gccgaaagcc acagtctgaa tctgacctat cgcggtaagg ataagccgac
    15421 caacgtgctc tccttcccgt ttgaagtgcc gcctggcatg gaaatgtcgc tactgggcga
    15481 tctggttatc tgccgtcagg tggttgagaa ggaagctcag gagcaaggca aaccactgga
    15541 ggcgcactgg gcgcatatgg tggtgcacgg cagtctgcat ttgttaggtt acgatcacat
    15601 cgaagatgac gaagcagaag aaatggaagc cctcgaaaca gagattatgc ttgctctggg
    15661 ctatgaggat ccgtacattg ccgagaaaga ataatttgcc aggcatttga ctggcagacg
    15721 tccccgccgt tggtgattaa cgacggcgtt ttgattaact aacatgagaa cccatacgac
    15781 gccatgagcg acgacaattc acacagtagt gacacgataa gcaacaagaa gggatttttc
    15841 tccctgttac tcagccaact tttccacggt gaaccgaaaa accgtgacga actgctggcg
    15901 ctgatccgtg attccgggca gaacgacctt atcgacgaag atacgcgcga tatgctcgaa
    15961 ggggtgatgg acatcgcaga ccaacgcgtt cgcgacatca tgatcccccg ctcccagatg
    16021 attaccctga aacgcaacca gacgctggac gaatgccttg atgtcatcat cgagtccgcc
    16081 cactcacgtt tcccggtgat tagcgaagac aaagatcaca ttgaagggat tctgatggcg
    16141 aaagacttgc tgccgtttat gcgcagcgat gctgaagcct tcagcatgga caaagtgtta
    16201 cgtcaggcgg ttgtcgttcc tgaaagtaag cgcgtagacc ggatgctgaa agagtttcgc
    16261 tctcagcgtt accacatggc gatcgttatt gacgaattcg gtggggtttc cggtctggtg
    16321 accattgaag acatcctgga actgattgtt ggtgagattg aagacgagta tgacgaagaa
    16381 gatgatatcg acttccgtca gctgagtcgt catacctgga ccgtgcgcgc actggcttcc
    16441 attgaagact tcaacgaagc gttcggcacc cactttagcg atgaagaagt cgacactatc
    16501 ggtggtctgg tgatgcaggc gtttgggcat cttccggcgc gtggcgaaac tatcgacatc
    16561 gacggttacc agttcaaagt ggcgatggcc gatagtcggc gtattattca ggttcatgtc
    16621 aaaatcccgg atgactcacc ccagccaaag ctggatgaat aaaaccgaaa ctggatagat
    16681 aactacatgg cttttgcctc attaattgaa cgccagcgca ttcgcctgct gctggcgtta
    16741 ttattcggtg cctgcggaac gctggccttc tctccttacg atgtctggcc tgcggcgatt
    16801 gtttcgctga tggggcttca ggcgctgacc tttaaccgcc gcccgctcca gtctgccgct
    16861 attggcttct gctggggatt tggcctcttt ggcagcggta ttaactgggt ctatgtcagc
    16921 atcgcgacct ttggcgggat gcctggcccg gttaacatct tcctggtggt gctgctggcg
    16981 gcgtatttgt cgctgtatac cggactgttt gctggcgtgc tgtcgcgcct gtggccgaaa
    17041 accacctggc tgcgcgtggc gattgccgcc cctgctctct ggcaagtgac cgagtttctg
    17101 cgcggttggg tgctgactgg cttcccgtgg ttacagttcg gctatagcca gattgatggc
    17161 ccgttaaaag ggctggcacc aataatgggc gtggaagcca ttaacttcct gctgatgatg
    17221 gttagcggcc tgctggcact ggcgttagtc aaacgcaact ggcgtccgct ggtggtggcc
    17281 gtcgtgctgt ttgcccttcc cttcccgctg cgttacatcc aatggtttac cccgcaaccg
    17341 gagaaaacca ttcaggtttc gatggttcag ggcgatattc cgcaatcact gaaatgggac
    17401 ggagaccagc ttcttaatac gctgaagatt tactacaacg caacggcacc gctgctgggc
    17461 aaatcatcgt tgattatctg gccggagtcg gcgataaccg atctggaaat taatcagcaa
    17521 ccgttcctca aagcactgga cggtgagttg cgtgataaag gtagctcgct cgtaaccggg
    17581 attgtcgacg cgcgtctcaa taagcagaac cgctacgata cctacaacac catcatcaca
    17641 ctgggtaaag gtgcaccgta cagctacgaa tcagccgatc gctataacaa aaaccatctg
    17701 gtgccgtttg gcgagtttgt cccgctggag tcgattctgc gtccgttagc accgttcttt
    17761 gatctgccga tgtcgtcgtt cagccgtggg ccatatatcc agccgccgct gtcggtaaat
    17821 ggtattgagc ttactgcggc catttgctac gagatcattc ttggcgagca agtgcgcgat
    17881 aacttccgcc cggataccga ctatctgctg actatctcca acgatgcgtg gtttggtaaa
    17941 tctattggtc catggcaaca cttccagatg gcgcgaatgc gtgcgctgga gctggcgcgc
    18001 ccactgttgc gcagcaccaa caacggcatt acggcggtga ttggcccgca gggtgagatt
    18061 caggcgatga tcccgcagtt cacccgcgag gcgttaacca ctaatgtgac gccaaccacc
    18121 ggactcacac catacgcacg taccggcaac tggccgctgt gggtgctgac ggcactgttt
    18181 ggttttgccg ctgtgctgat gagtctgcga gcacgtaaac gttgatgtgc ctgatgtatc
    18241 ctgcatgagc aaaacgtact ttctcattga gctttggggc atggttatca tgcccctttt
    18301 tcttacggtt cagggataca agccacattg ccatattggg gatgacgaat aattgcctgc
    18361 ggatcaagcg agcgcgtcct gtccagttgg atcaacccgt tcttcgccat aacaaatatt
    18421 tttctaccat ccgctgaaaa acgaaggtga ttaacgtagc tggcaggata aacgccaata
    18481 atctgcccgg cattaatatt gagcaggatg acataacctt gcaggccata tgacaccaac
    18541 gctaaatact gaccgtccgg gctaataccc tcaacggaat aaacgctcct gctacaccat
    18601 tgcgtcataa tgctggcttg ttgatcccct acgcttatac gtgaaatccc ctggctgtcg
    18661 gcaaaaatta catccccgtt ttcaggtcga aaacgcatcg ccgatatcgt tcctcgcgca
    18721 tcactagccg gatagctttc tttctgatgc gtgatggtat tgatgcgtac aatgtcacct
    18781 ctgaccatac cgggtatcaa caatattttt tcatccgggg aaagagccat aacccaggca
    18841 caaggtttat tactcgtccc cgtcccgtcg tatttctcca gcgggatatc cgccacatgc
    18901 tgataagtca ccagatcaat cgctgacaca ctattactgg tgcgattcat cacccaggct
    18961 tttgtaccat cacgcgagag gataaggctg tcaggatgtg caccggtggt aatcgactga
    19021 ataatctccc ctgtcgccac gttaatgacc gataacgcat tcgcttcaaa tgaagtcgca
    19081 taaatcaact tttcatccgg gcttaatgcc agcgatctga cgccctgatc tttacagcca
    19141 ccacaatgtt tgactgactt ttctttcgct atgggcgctg taaaggtatg caggattttc
    19201 ttttgtccga ggtcgaaaac aacaatgctg ttggtgtttt caattgcggc atacaacata
    19261 ttaccatccc ggctgatcgc catatcatgg atatcggcaa tctcagcgag caaatcacca
    19321 ctggcctgtt cacgacttac cggagcggat aatgtttccc tgagggcgtt gcgttcctcc
    19381 gctaactgct gctttttttg ccgtcgctct tgttcaatct cctgtggata aaacgcttca
    19441 cgcaggcttt gcctgacttg taaaactgac tcatccatct gctgctgcgc aagccagata
    19501 gccccggtca taagaagact gcccgcaaca ataatcccgc cttccgtaac gagtcggttt
    19561 tgcccagata aacgcgcgct cagccaaccc aacgcgccac ccgcaatcag gccgccaatg
    19621 tggcaagcgt tatctattcc tgtttgccgg gcaccgtaca acagtgtcag cgccaccata
    19681 cccaacagag gaaatactcg ccgctggttt ttatggtatg tgcccgtacc tgaagcaaac
    19741 tgtgttgcta tcgctgcacc agctattccc agaatcgccc cggatgcccc aacgctgatg
    19801 actatctgct cactttcgcg taacgcataa caggcgctga tgagcccacc aaagacgccg
    19861 gagaataacc aggtaatcag gagtcgccat ttccccagaa tctgttccgc cacgcctccc
    19921 acgaccagga atgcaaacat attcatcagc agatgagaaa cactactgtg cagaaagata
    19981 ctgctcaata agcgccaggg ttcacccgac aaagtaagag gtgcaacgtt tgcgccgaga
    20041 tggataagtg cggattgatc gacctcttca taactcatcc attgtgcgag ataacaaaat
    20101 atcccaatac agagtgcaaa aaggataagg gttattgcgg gtaattgaac atttaacggc
    20161 ttaaccgaag atgcagacat aaggtgtttc ttccttgaaa agacaggtaa aaagcatttt
    20221 tcccgtgtgc agttatcatc ccggcaaacg tcccatgccg aaaagataat tctgtccgaa
    20281 atatgacttt ttcagcctaa aattacaatc gtaattaatt cgcccctaag tattttttgg
    20341 catgtaagtt gctttattta ctgtgtaagc gatgaaatca gccgcgacgc aaccaggttg
    20401 caccaccaca aatcagcggt agcaccaaaa aggtgcggtt taagaatttt gcttcggaat
    20461 ggtgcggcac atctcgcaaa aacaaacaat aaaacagcaa tatctttaca tttagccaaa
    20521 ctaaatgtta cctaacaggc acaacactgc acaataaagt tgcagacgat aacaacacaa
    20581 acactcacaa cgggtatcca tgcgttctta acgcagaaga taaaggagtt ggatatgcaa
    20641 ttacgtaaac ctgccacagc aatcctcgcc ctggcgcttt ccgcaggact ggcacaggca
    20701 gatgacgccg ccccggcagc aggcagtacg ctggacaaaa tcgccaaaaa cggtgtgatt
    20761 gtcgtcggtc accgtgaatc ttcagtacct ttctcttatt acgacaatca gcaaaaagtg
    20821 gtgggttact cgcaggatta ctccaacgcc attgttgaag cagtgaaaaa gaaactcaac
    20881 aaaccggact tgcaggtaaa actgattccg attacctcac aaaaccgtat tccactgctg
    20941 caaaacggca ctttcgattt tgaatgtggt tctaccacca acaacgtcga acgccaaaaa
    21001 caggcggctt tctctgatac tattttcgtg gtcggtacgc gcctgctgac caaaaagggc
    21061 ggcgatatca aagattttgc cgacctgaaa gacaaagccg tagtcgtcac ttccggcact
    21121 acctctgaag ttttgctcaa caaactgaat gaagagcaaa aaatgaatat gcgcatcatc
    21181 agcgccaaag atcacggtga ctctttccgc accctggaaa gcggtcgtgc cgttgccttt
    21241 atgatggatg atgccctgct ggccggtgaa cgtgcgaaag cgaagaaacc agacaactgg
    21301 gaaatcgtcg gcaagccgca gtctcaggaa gcctacggtt gcatgttgcg taaagacgat
    21361 ccgcagttca aaaagctgat ggatgacacc atcgctcagg tgcagacctc cggtgaagcg
    21421 gaaaaatggt ttgataaatg gttcaaaaat ccaattccgc cgaaaaacct gaacatgaat
    21481 ttcgaactgt cagacgaaat gaaagcactg ttcaaagaac cgaatgacaa ggcactgaac
    21541 taattacaag aaccaggggc ggaaattcca gccctctcga ttgttacgta gcacggacag
    21601 actatacgcc tgatggtcgt tccccatcgg gcctgaaaac cgcaatacgc tgggtaacaa
    21661 tcttcgaggg tagcagttaa cgctgctacc ctcttttttc tggagtagat ttatgtctat
    21721 agactggaac tggggtattt ttttacaaca agccccgttc ggcaacacca cctatcttgg
    21781 ttggatctgg agtggttttc aggtgacgat cgctttgtcg atctgcgcct ggattatcgc
    21841 tttcctcgtc ggttcatttt tcggcatttt acgtaccgtt ccaaaccgtt ttctctccgg
    21901 tctgggtacg ttgtatgtcg aactgttccg caacgtgccg ttgattgtgc aattctttac
    21961 ctggtatctg gtgatcccgg agctgttgcc ggagaaaatc ggcatgtggt ttaaggccga
    22021 gctggatccc aatattcagt ttttcctttc atccatgctc tgcctggggc tgtttaccgc
    22081 cgcccgtgtt tgcgaacagg tacgcgccgc cattcagtcg ttgccgcgtg ggcaaaaaaa
    22141 tgccgcgctg gcgatgggcc tgacgctacc gcaggcttat cgatacgtac tgctgcctaa
    22201 tgcttatcga gttatcgtcc cgccgatgac ttccgagatg atgaacctgg tgaaaaactc
    22261 cgccatcgcc tcgactattg gtctggtgga tatggcggcg caggcgggta aattgcttga
    22321 ttactcagcc cacgcctggg agtcgtttac cgctattacg ctggcgtatg tgttgattaa
    22381 cgcggttatc atgctagtaa tgacgctggt tgaacgtaaa gttcgcctgc ctggcaacat
    22441 ggggggcaaa taatgtacga gtttgactgg agttccattg tcccttccct gccatatctg
    22501 ctcgacgggc tggtgatcac gctgaaaatc accgtcacgg cagtcgtgat tggtattttg
    22561 tggggcacca tgctggcggt gatgcgcttg tccagctttg cgcccgtagc gtggtttgcc
    22621 aaagcctacg ttaacgtatt ccgctcgatt cctttagtta tggttttgct gtggttttac
    22681 ctgatcgtgc caggttttct gcaaaacgtg ctgggattat caccaaaaaa tgatatccgt
    22741 ttaatttcgg cgatggtggc gttttcgatg tttgaagcgg cctactattc agagattatc
    22801 cgtgctggta ttcaaagtat ttctcgtggt cagtcgagcg ccgcgctggc gttgggaatg
    22861 actcactggc agtcgatgaa actgattatt ctgccgcagg cgttccgcgc gatggtgccg
    22921 cttctgctca ctcagggcat cgtactgttc caggatacct cactggtgta tgtgttatct
    22981 ctggccgatt tcttccgtac cgcctcaacc attggtgagc gtgatggtac gcaggttgag
    23041 atgatcctgt ttgccggatt tgtttatttc gttattagtc ttagcgcgtc gttgttggtc
    23101 agctacttga aaagaaggac agcataatga ttaccctgaa aaatgtttca aaatggtatg
    23161 gtcactttca ggtgctgacc gactgctcaa ccgaagtgaa aaaaggcgaa gtggtggtgg
    23221 tttgcggccc gtctggttcc ggcaaatcaa cgctgattaa aaccgtcaac ggcctcgaac
    23281 cagtgcagca aggtgaaatc accgtcgatg gtatcgtggt taatgacaag aaaaccgatc
    23341 tggcaaagct gcgttcccgc gtcgggatgg tattccagca tttcgagctg ttccctcatc
    23401 tgtcgattat cgaaaacctg accctggcgc aggtgaaagt gcttaaacgc gataaagcgc
    23461 ccgctcgtga aaaagcattg aaattgctgg agcgcgtggg actttccgca catgccaata
    23521 aatttccggc gcagctttcc ggcggtcagc agcagcgtgt ggcaatcgct cgcgcgttgt
    23581 gtatggatcc tattgcgatg ctgttcgacg aaccgacatc ggcgctggat ccggagatga
    23641 tcaacgaagt gctggacgtg atggtggaac tggcaaacga aggaatgacc atgatggtgg
    23701 tgacccacga aatgggtttt gcccgtaaag tggcgaatcg ggtgatcttt atggacgagg
    23761 gtaaaattgt cgaagactcg ccgaaagacg ctttcttcga tgatccgaaa tcggaccgcg
    23821 caaaagactt cctcgcgaaa atcctgcatt aatcactctg gcgcgcatac ccaattgcgc
    23881 gccatctcac gctttatcgt ttcgcccctc gtatctgtta catcctggcg ttacccttgt
    23941 cgcaaagaag cacacaacaa ggagcaacaa tggcactgcc aattctgtta gattgcgacc
    24001 caggtcatga tgacgctatc gcaatagttc tcgccctcgc ctcaccagag cttgatgtca
    24061 aagcaattac gtcttccgcc ggaaaccaga caccagaaaa aaccttacgc aatgttctgc
    24121 gtatgctgac cttgcttaat cgcaccgata ttccggtagc cggcggcgcg gtaaaaccgt
    24181 taatgcgtga gttgattatc gcggataatg tgcacggcga aagcggactt gacggcccgg
    24241 cactaccgga accgacgttt gcaccgcaaa agtgtacggc ggtggagctg atggcgaaaa
    24301 cgctacgtga aagtgcggaa cctgtcacga ttgtgtctac cggaccgcaa actaacgttg
    24361 ccttgctgct caatagccat ccggaactgc atagcaaaat tgctcgtatc gtgattatgg
    24421 gcggcgcaat ggggcttggt aactggacgc ctgcggctga atttaatatt tacgttgacc
    24481 cggaagcggc agaaattgtc ttccagtcag ggatcccggt ggtgatggcc ggtctggatg
    24541 ttactcataa agcacaaatc cacgttgaag acaccgagcg tttccgcgcg attggcaacc
    24601 ctgtttcaac cattgttgcc gaactgctgg atttcttcct cgaatatcat aaagacgaaa
    24661 aatggggctt tgtcggtgca ccgctgcatg acccatgcac catcgcctgg ctgttgaaac
    24721 ccgagttgtt taccactgtt gagcgctggg ttggcgtgga aacacagggg aaatatagcc
    24781 agggtatgac ggttgttgat tattattatc tgactggcaa taaaccgaat gccactgtaa
    24841 tggtcgatgt tgatcgtcag ggctttgttg atttactggc tgatcgtctg aaattttacg
    24901 cttaacacac gtcattgatt aagatcacca cgcgttacct gggtggtctt aatctgttta
    24961 tctctttttc tttcaagata taaatgcaat ggataatgca gaactcgcca ttggtatcga
    25021 tctcggcact accaatagtt taattgccgt ctggaaagac ggtgccgcgc aattaattcc
    25081 aaataagttc ggtgaatatt taacaccatc cataattagc atggatgaaa ataatcatat
    25141 tttagtcgga aaaccggctg tatcacggcg tacttcgcat ccggataaaa cggcagcgtt
    25201 atttaaacgt gcaatgggca ctaataccaa ctggcggtta ggcagcgaca catttaacgc
    25261 gccagaactg tcctctttgg tattacgctc attaaaagaa gatgccgaag aatttctgca
    25321 acgtccgatt aaagatgtgg tgatctccgt tccggcttat ttcagcgatg aacaacgcaa
    25381 gcatacccgt ttagcagcgg agttagccgg gttaaatgcg gtacgcttaa ttaatgaacc
    25441 cacagcagct gcgatggcat atggcctgca tacgcaacaa aatacccgct cgctggtttt
    25501 tgatctcggt ggcggcacgt ttgacgtcac ggtgctcgaa tacgccacgc cggtgattga
    25561 agttcacgcc tccgctggcg ataacttcct cggtggcgaa gattttaccc atatgttggt
    25621 cgatgacgtt ttaaagcgcg cggatgtcgc caggaccacg cttaacgaga gtgaactggc
    25681 agccttgtac gcctgtgtgg aagcggcaaa atgtagcaat caatcgccat tgcacattcg
    25741 ctggcagtac caggatgaaa cgcgggagtg cgaattttac gagaacgaac tggaagattt
    25801 gtggttgccg ctgctcaatc gcttgcgagt gccgattgaa caggcgttgc gcgatgcacg
    25861 tctgaagccg agtcaaatcg acagtctggt gctggttggc ggcgcgtcac aaatgccgct
    25921 ggtgcagcga atcgccgtgc gtctgtttgg caaattaccg tatcaaagtt acgacccgag
    25981 caccattgtc gcgctgggcg cagcaatcca ggccgcctgc cgcttacgca gtgaagatat
    26041 tgaagaggtg atcctcactg atatttgccc ttactcgttg ggcgttgaag ttaaccgcca
    26101 gggcgtttcc ggcattttct cgccaattat tgaacgaaac accacagtac ccgtgtcgcg
    26161 tgtagaaact tactcgacca tgcacccgga acaggattca attacggtta acgtctatca
    26221 gggagaaaac cacaaagtta aaaataacat tctggttgaa tccttcgatg tgccgttgaa
    26281 gaaaaccggg gcttatcagt cgattgatat tcgctttagt tatgatatca acggcttgct
    26341 tgaagttgac gtgcttctgg aagatggcag cgttaagtcc agagtcatta atcatagccc
    26401 ggtaacattg agcgcgcagc agattgaaga gagtcggacg cggttatccg cattgaaaat
    26461 ttatccgcgc gatatgctca tcaaccgcac ctttaaggcc aaactggaag agttgtgggc
    26521 gcgggcgctg ggtgacgagc gagaagagat tggccgggtg atcaccgatt ttgatgcggc
    26581 gttgcagtca aacgatatgg cccgcgtcga tgaggtcagg cggcgggcga gcgtttattt
    26641 agccattgag acctcataat cacggctgcc ggatgcggcg taaatgctgc atccggcatc
    26701 ggcgcaaaaa acacatcgtc aactaaacaa catcgcgatt ttgccgagag cataacctat
    26761 aaccagcacc gttaaactga ttttgataat gctggtaatg ctctgtggtt tttcaccctc
    26821 atcatttttt tcttcatggt tttcaggtgt tggctcatcg acatcttcat cctccggctc
    26881 gagttttact ttcgtcaaca gcatccgggt gataatggcc tctaatggat gagcatctcc
    26941 gccttccagc cttgtattaa gacgggccag catctcttgt ttcggaaacg cttcaagtac
    27001 tacctgttcc gctacccgat acaaatccag taacggtgaa tcttcttctg tctcgctacc
    27061 gactctcgat ttccaccacg cccatgcttt gcgtttgaag aataacgact caataaacga
    27121 ttgacctaac ggcgataatt ttccgctatg taaaatttcc gcccataaca gccgcgtccg
    27181 ggcgctctca ccaggaacat atttcagcgg ttgaccttgc gcatctacca tgctccgcgc
    27241 ttcgataacc atcactaata acgcgaaata atccgggcaa tgacgcttgc tccactgcaa
    27301 caacaaatta tcagcttgtg tagatgggta actttcccgg tacgcgcaga gatcggcaag
    27361 caggctttcc ccctcgccgc agtacaacct ttgagcacac aagtaatatc gggcatcttc
    27421 gctttctggt tcttccattt gccactgttt ggctacagga atgagttccg ccatacctgt
    27481 ttgcaccgag ctgtaccagc gcaacagttt gcgatgtaac tttgcatcat caggaatgaa
    27541 ccacggacct tccatcgcca aaaacgcagt gacgtagtca ggatggtaac ggcagcaacg
    27601 ttccagcgcg aagtaatact caacagtctg gttctgcaac gcaacgggat attccagaag
    27661 gctaaggaaa tcgaaacaat caccagcctg aatggcctca agaaaagcct ccctttcttc
    27721 ctcatccatt ccttcgctgt cattaaacga atgccagttc aaacgctctg ccagcaaaga
    27781 agcacaattt aatgaaagat atcgcgcttc tatagcgatt gcacacagcg gccagcgtaa
    27841 ttgatcgaca tcctccatgt tccaggtatt taattgctga ataaatttct gccaggcgga
    27901 gggctgaaaa cgatcacttt ccgaatccag taatgtcctg aatgcacgca gtatttcatg
    27961 ttcagcggcg gcatcttttt cttcatcatc tgcttcctcg acaggattta ccgccagtcg
    28021 cagcgcctct tcataggcct ggcgtaatgc tttaaacccc tgcggatcgg tttcgggatg
    28081 acataacggt aagcgagcaa gataagcctg gcggataatg tctatttgcg tcgtgctttc
    28141 aatttcgagg atttgccaac atgttttcat aggtcgctca catccttaag cgttaccggg
    28201 cattcaggct ggacagttaa tggctgccag ggaagcgtgg catagaaacg atcgcttccc
    28261 gctgccagct cttcgaacat ccgactcccg gcgctatcgt atttgtacag ggttagcgcc
    28321 tcgcgtagcg aagtgttgtc ttcgcactga gaagattgcc agtagagacg tcctaacgaa
    28381 taggcggaaa aatagtgcat ccagctgtga tagtaatggt gagctcgtac atagacgcgc
    28441 gattgcagcc ataaactctc ttcttccgtc agccagttgt tgagcacacc gacccgtgac
    28501 agaaacccca ttcgaacata atcccaggct ttgattcccc cttctccaca gcacatagcg
    28561 gtttgggcaa cgaattgggt ataaatcaac cctctctccg agccaccagc aattaatgct
    28621 ttccactctt caggggaaga gcgatgccat gtcagatata agccagctaa atcattggca
    28681 tgaccatcat cagtcatctg ataaatagtc tgaattaaat catcacgatt atttattccc
    28741 caggatgatg agagatcgaa ttcttttata ccggtataca tttttggcga gtcatgcgcg
    28801 ggtttgcaac cctgattaag tatttccatg ggcgcagaaa gcgcaaaaag aagatattgt
    28861 tcttccttat tcatttgtta ataatccctt aagtg
//
LOCUS       DAZGVQ010000056        28259 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000056 DAZGVQ010000000
VERSION     DAZGVQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.056"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..209)
                     /locus_tag="ACHLAG_002297"
     CDS             complement(<1..209)
                     /locus_tag="ACHLAG_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP0655515.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHLAG_002298"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHLAG_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP0655516.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHLAG_002299"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHLAG_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFP0655517.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHLAG_002300"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHLAG_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFP0655518.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHLAG_002301"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHLAG_002301"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFP0655519.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHLAG_002302"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHLAG_002302"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFP0655520.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHLAG_002303"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHLAG_002303"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFP0655521.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHLAG_002304"
     CDS             complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHLAG_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0655522.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHLAG_002305"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHLAG_002305"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP0655523.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /gene="slyX"
                     /locus_tag="ACHLAG_002306"
     CDS             6520..6738
                     /gene="slyX"
                     /locus_tag="ACHLAG_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFP0655524.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHLAG_002307"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHLAG_002307"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP0655525.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHLAG_002308"
     CDS             complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHLAG_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFP0655526.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ACHLAG_002309"
                     /pseudo
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ACHLAG_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHLAG_002310"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHLAG_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFP0655527.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /gene="yheS"
                     /locus_tag="ACHLAG_002311"
     CDS             10169..12082
                     /gene="yheS"
                     /locus_tag="ACHLAG_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0655528.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDDAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /gene="yheT"
                     /locus_tag="ACHLAG_002312"
     CDS             12082..13104
                     /gene="yheT"
                     /locus_tag="ACHLAG_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP0655529.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /gene="yheU"
                     /locus_tag="ACHLAG_002313"
     CDS             13098..13316
                     /gene="yheU"
                     /locus_tag="ACHLAG_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFP0655530.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /gene="prkB"
                     /locus_tag="ACHLAG_002314"
     CDS             13370..14239
                     /gene="prkB"
                     /locus_tag="ACHLAG_002314"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFP0655531.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKID"
     gene            complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHLAG_002315"
     CDS             complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHLAG_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFP0655532.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="ACHLAG_002316"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="ACHLAG_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFP0655533.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHLAG_002317"
     CDS             15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHLAG_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFP0655534.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHLAG_002318"
                     /gene_synonym="dapC"
     CDS             complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHLAG_002318"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFP0655535.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGLGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLSCAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAGAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHLAG_002319"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHLAG_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFP0655536.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHLAG_002320"
     CDS             complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHLAG_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFP0655537.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHLAG_002321"
     CDS             complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHLAG_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFP0655538.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHLAG_002322"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHLAG_002322"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFP0655539.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHLAG_002323"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHLAG_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFP0655540.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYIAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="ACHLAG_002324"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="ACHLAG_002324"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFP0655541.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="ACHLAG_002325"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="ACHLAG_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFP0655542.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25884..26690
                     /gene="nirC"
                     /locus_tag="ACHLAG_002326"
     CDS             25884..26690
                     /gene="nirC"
                     /locus_tag="ACHLAG_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFP0655543.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26709..28082
                     /gene="cysG"
                     /locus_tag="ACHLAG_002327"
     CDS             26709..28082
                     /gene="cysG"
                     /locus_tag="ACHLAG_002327"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFP0655544.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6843 a         7589 c         7131 g         6696 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gataatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ctcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc ccccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatat tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctttt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgcg tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacgt ccaacagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagagagc ggttcaccac gagtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagagt acctttgtag ttcacgacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttcttttag agagttttcc
     6121 atgtaacgac ccagcgaggc acccagtgca taagctgact tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcaaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgactgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccgc attattgagg cgaaaaaaaa gcggggaatc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtggtgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tggtgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgttgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccagt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcagcgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taagcgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata caacacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaacg
     8761 taccgagcgc catcgacagc cccagcgcgt ccataaacaa tgcggaaccc aacaccaaca
     8821 gcagcgtcgc ggcggtgaac acttaccgca cgccagaagc tgcaataaaa cggaataccg
     8881 gacgcagtaa atagcgcccg ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg aacccgccaa caacggcact aacgccagtg
     9001 ccgggatcac cgccagatcc tgaaacagca gaaccgaaaa cccgagctgc ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgacaacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa gaccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccatgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 gcggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtgactcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaaggga cgcagaacat cgctcatcgg
     9661 gtagcgattc aatgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgct
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcataaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcaccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccctgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaacta gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtatgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaaag tcagcgcggg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaac ctggtgcccg gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctcaggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagttcgaag gcgcgctggt tgtcgtctcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgacctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 cgcgccaaaa gagaatgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccactgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc
    12121 agcagtgctg aattcatccc catgcgcggc tttagcaact gccatctaca aactatgctg
    12181 ccgcgtctgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct tgcgtggagt gaaaaccctg cacaggcgca acataaacct
    12301 cgtctggtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaagaag gcaatgatct ccctgttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcacgc
    12721 aagctggcag cctaccccgg aacactgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctaatcacc gccagaattc acggctacgc tgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa acctacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggttgagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatca tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccctgg aatcaggtac cggggacatt caccccctgg caacctttac cggaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctcgatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtaccggg
    14161 cggcaaaatg ggtctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgactaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcgga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta
    15721 tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccattac ttccggcgct
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actattgtgg ttgaacggcg agcaatacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggcg gtactgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattcttag
    17281 cgaggatccg caacctacgc cactggcctg gcagcgaatg cgggtaaatc aggcacataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcgtttaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat tcacgccgca tccgacatct
    17821 tttgcctgat gcttcgtatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggtgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cccctgcgcc cgcatacagg aaatcacgcg
    18001 cccgaccttt gaactgtggc ttaagctccg cgccaatcaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcagatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc gcgcccgcta
    18181 ctgcacagga cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaatgcag
    18241 aagcaatttc tgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaggtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccggtcc
    18361 gccccatccc gcactgtact tcatcaaaca ccaataatgc ctgatgttga tcgcacagct
    18421 cgcgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt
    18541 taaaaggaac gtggatgatg tctgccggtt ttggcccaaa gccgtcggaa tattttggct
    18601 gcccgcccac cgagacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacggacta tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttgcg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgcccagacc ttttaccgga atgaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttacg
    19081 cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcattg aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacgtcaaag catgctggta atgagccagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttgccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccgggt
    19561 gatatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gcgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggct tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagtcc
    19921 gcttcccaca cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ccgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggtggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgaaaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcggcg gcgatgttca ttctcggggt ggtcagcggc
    21781 atcaccatgt cgattggtac attcctggta acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttaccga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatatcg ctatttttat tctgaccttc ggctgtgagt tcccggcgct gggcaaacat
    22021 gcgccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccagcac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc tattttttgc acaaacatga
    22741 aatatcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg gttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt
    23221 tcctacccgt ggatcccgcc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaagcg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctcggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggtaacctgc tgcaactggt gctgaacgct
    24061 atcgaactgc cggaaaaccc ggattccctg atcctgccag cacactctgg tagcggcaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgcc acaccgttgc ggcgctgaaa
    24241 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggaa cctactcggt gatcccgcgc
    24601 tctccgggcg gtgaaatcac cccggaagga ctgatggcag tgggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaaaat caccggctct cagcgcctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggagatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactacgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggc
    24841 gttggcgaca gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25141 gcgccgtggt tagaaaacct cgaaggcggc atcgattatc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc acatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgta cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtagcaatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca
    25561 acatcgaccc gttcttcgag tccagcgtgc tgtcacgcgg gctgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta tttttttgtt
    25861 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaacg
    25921 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25981 tggcgggcgc gtatgtgggt cttgggatca tcttgatttt cacgctcggt aatttgctcg
    26041 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26101 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26161 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26221 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26281 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26341 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26401 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26461 ctggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26521 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26581 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26641 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacggc tgccggttaa ttactaaggg
    26701 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26761 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26821 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26881 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26941 cagcgacgga tgatgacgcg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    27001 tcttctgtaa cgtggtcgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27061 accgctcacc gctcatggtc gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27121 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27181 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27241 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27301 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27361 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27421 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27481 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27541 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27601 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27661 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27721 cctattcggg tattccgctc acgcatcgcg attatgccca gagcgtgcgc ttaattaccg
    27781 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27841 tggtgttcta tatggggctg aatcaggctg cgactattca gcaaaagctg attgaacacg
    27901 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacagcagtc acgcagcgcg
    27961 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    28021 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaaccatt
    28081 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28141 ataaaacata gccccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28201 acttacaaaa ttaagtcatt cgctcactga cgccaattaa gtattcctgt atcgttctt
//
LOCUS       DAZGVQ010000057        28030 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000057 DAZGVQ010000000
VERSION     DAZGVQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.057"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(92..1567)
                     /gene="ampG"
                     /locus_tag="ACHLAG_002328"
     CDS             complement(92..1567)
                     /gene="ampG"
                     /locus_tag="ACHLAG_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFP0655545.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(1611..2189)
                     /gene="yajG"
                     /locus_tag="ACHLAG_002329"
     CDS             complement(1611..2189)
                     /gene="yajG"
                     /locus_tag="ACHLAG_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0655546.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            2494..2811
                     /gene="bolA"
                     /locus_tag="ACHLAG_002330"
     CDS             2494..2811
                     /gene="bolA"
                     /locus_tag="ACHLAG_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFP0655547.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            3155..4453
                     /gene="tig"
                     /locus_tag="ACHLAG_002331"
     CDS             3155..4453
                     /gene="tig"
                     /locus_tag="ACHLAG_002331"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP0655548.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            4699..5322
                     /gene="clpP"
                     /locus_tag="ACHLAG_002332"
     CDS             4699..5322
                     /gene="clpP"
                     /locus_tag="ACHLAG_002332"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP0655549.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            5448..6722
                     /gene="clpX"
                     /locus_tag="ACHLAG_002333"
     CDS             5448..6722
                     /gene="clpX"
                     /locus_tag="ACHLAG_002333"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFP0655550.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            6910..9264
                     /gene="lon"
                     /locus_tag="ACHLAG_002334"
     CDS             6910..9264
                     /gene="lon"
                     /locus_tag="ACHLAG_002334"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFP0655551.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINSDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            9455..9745
                     /gene="hupB"
                     /locus_tag="ACHLAG_002335"
     CDS             9455..9745
                     /gene="hupB"
                     /locus_tag="ACHLAG_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFP0655552.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            9937..11808
                     /gene="ppiD"
                     /locus_tag="ACHLAG_002336"
     CDS             9937..11808
                     /gene="ppiD"
                     /locus_tag="ACHLAG_002336"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP0655553.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAQQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            11959..12330
                     /gene="ybaV"
                     /locus_tag="ACHLAG_002337"
     CDS             11959..12330
                     /gene="ybaV"
                     /locus_tag="ACHLAG_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFP0655554.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            12424..12822
                     /gene="fadM"
                     /locus_tag="ACHLAG_002338"
     CDS             12424..12822
                     /gene="fadM"
                     /locus_tag="ACHLAG_002338"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFP0655555.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(12874..13569)
                     /gene="queC"
                     /locus_tag="ACHLAG_002339"
     CDS             complement(12874..13569)
                     /gene="queC"
                     /locus_tag="ACHLAG_002339"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFP0655556.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(13634..15334)
                     /gene="ybaE"
                     /locus_tag="ACHLAG_002340"
     CDS             complement(13634..15334)
                     /gene="ybaE"
                     /locus_tag="ACHLAG_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFP0655557.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGNSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPYWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            15434..16252
                     /gene="cof"
                     /locus_tag="ACHLAG_002341"
     CDS             15434..16252
                     /gene="cof"
                     /locus_tag="ACHLAG_002341"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFP0655558.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            16405..16863
                     /gene="decR"
                     /locus_tag="ACHLAG_002342"
     CDS             16405..16863
                     /gene="decR"
                     /locus_tag="ACHLAG_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFP0655559.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            16893..18665
                     /gene="mdlA"
                     /locus_tag="ACHLAG_002343"
     CDS             16893..18665
                     /gene="mdlA"
                     /locus_tag="ACHLAG_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP0655560.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVAQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWEQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            18658..20439
                     /gene="mdlB"
                     /locus_tag="ACHLAG_002344"
     CDS             18658..20439
                     /gene="mdlB"
                     /locus_tag="ACHLAG_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP0655561.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDER
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            20620..20958
                     /gene="glnK"
                     /locus_tag="ACHLAG_002345"
     CDS             20620..20958
                     /gene="glnK"
                     /locus_tag="ACHLAG_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFP0655562.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            20988..22274
                     /gene="amtB"
                     /locus_tag="ACHLAG_002346"
     CDS             20988..22274
                     /gene="amtB"
                     /locus_tag="ACHLAG_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFP0655563.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(22322..23182)
                     /gene="tesB"
                     /locus_tag="ACHLAG_002347"
     CDS             complement(22322..23182)
                     /gene="tesB"
                     /locus_tag="ACHLAG_002347"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFP0655564.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            23400..23972
                     /gene="ybaY"
                     /locus_tag="ACHLAG_002348"
     CDS             23400..23972
                     /gene="ybaY"
                     /locus_tag="ACHLAG_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFP0655565.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(24003..24314)
                     /locus_tag="ACHLAG_002349"
     CDS             complement(24003..24314)
                     /locus_tag="ACHLAG_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFP0655566.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            24477..24573
                     /gene="ffs"
                     /locus_tag="ACHLAG_002350"
     ncRNA           24477..24573
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHLAG_002350"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            24693..25046
                     /gene="ybaA"
                     /locus_tag="ACHLAG_002351"
     CDS             24693..25046
                     /gene="ybaA"
                     /locus_tag="ACHLAG_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HFP0655567.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAATQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(25088..26638)
                     /gene="pdeB"
                     /locus_tag="ACHLAG_002352"
     CDS             complement(25088..26638)
                     /gene="pdeB"
                     /locus_tag="ACHLAG_002352"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFP0655568.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(26802..27272)
                     /gene="ylaC"
                     /locus_tag="ACHLAG_002353"
     CDS             complement(26802..27272)
                     /gene="ylaC"
                     /locus_tag="ACHLAG_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFP0655569.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(27388..27939)
                     /gene="maa"
                     /locus_tag="ACHLAG_002354"
     CDS             complement(27388..27939)
                     /gene="maa"
                     /locus_tag="ACHLAG_002354"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFP0655570.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
BASE COUNT         6989 a         6785 c         7636 g         6620 t
ORIGIN      
        1 caaaagaaca aatattatta agcgatactg atgtttattt agccgtaata attacggcga
       61 gtgattacta cagctaaata atatttacag attacgtcag atgcgttttt cgtagcgcca
      121 gataatccag caaaccacca agcacgacgc cagaaagggc gactaaaacc ccgacttcca
      181 gcagagcagg caggaaagag aagtgcgtca aatccagcgc gtccatcgtc aacagcagta
      241 accacacggc caacaggctg acgcccgccg ccagtgtcca catggcaaag gcataacctg
      301 ccggatattc ggtacgggag ataaagttgt catttactcg tgtatattca agcgtctggc
      361 ggcaaaccag cagcaaaata agccccggta cggcagcggc gacggagaat agatagaatg
      421 tcgaccagcc gtgtgcttca acaaaccaac ccgccacggg gccgacataa actcgcccta
      481 cagcggaaag cgctgagagc agggcaaatt gagttgcgga aaatgactta ttacacagcg
      541 tcattaacag cgcgacaaag gctgatgtgc ccatcccgcc acagaggttt tcgaaaaaga
      601 cggctgcgcc catgctgtag agatgcttat cagtaatcga cagcagccag taaccagcgt
      661 tagacgcacc ttgtaaaatg ccgaaaatca gcagcgcccg gaacagcgac aggcgctgca
      721 tcaaaatccc accgtacaat gcgcccacaa tggtcgccag taagccaagc gttttgttaa
      781 ccacgcctac ttcacccgca tcaaacccga cgccgcgaat caaaaacgtg gttgtcaggc
      841 tcatggcgaa tgcgtcgccc agcttataca gcacgataag aagcaaaata agccaggcat
      901 tattgcgacc aaagaaatct cgcagaggtg caacaaccgc ttgttccagc gtttttggca
      961 caggaatggt gtcggtcggt tctggtgcaa gcaacgtcgc gataatacaa gggatcaaca
     1021 gcgccgccat caaccagtac atgccctgcc agcccagcca tttatctgcc agccacaggg
     1081 ctaagccgcc ggaaaccagc atccctaaac ggtaacccag cacgctgatt gccgcgcccg
     1141 caccacgttc ttctgccggg agcacatcgg ttttccacgc atcgaagaca atatcctggg
     1201 aggccgagca aaaagcgatc accaccgcca gtgccgccat ccagcggagt tgggtgccag
     1261 gttcgagaaa acccatcgcc gcaatggcga ctaataacag gatttgcgtg gcgagcagcc
     1321 aaccgcgccg ccgcccaaaa aatgggggcg tgtagcggtc catcagcggt gaccagagga
     1381 atttaaaaac gtaagcctgg cctaccagag agaagaaacc aatggttttg agatcaatat
     1441 tctcgaccgt catccaggcc tgtaaggtgc cggatgtcag ggcgagcggt agcccggaag
     1501 caaaacccag gatcagcaat atggctgaac gcggctgttg aaaaatacgt aaatattgac
     1561 tggacatggg cgtctacagg cccggtttgc accgggccag agggcagaaa ttaacgcgcg
     1621 ttctgcttga taaattcgtg aatgctggtg tcctgagaca tatcagcgat ggtatcggtc
     1681 agcacactgt taaccgcatc ggcgatattt ttgttggagg cctggaacgc accttcaacg
     1741 ttgtagctgg cacgatagtt tttggtcatt ttgttgccat tctgcgcggt agcgatgatg
     1801 gcgatatccg ctttggtcgc gatgttatag cgcacgttgc cctgggacac gtcagcatac
     1861 agttggctaa cgatgatttg cagattaacc gggccattcg gaccaaccat gtaaccacgc
     1921 gcggtcatct gtttttccag cacttcttgc agcaggaaac gcagatcgcg ggaggcggtc
     1981 agggtaacga tttgattatc gcgggtgact tttgccagcg cctgatcggt acgctgatcg
     2041 gcaccattaa tgcttacggt gacgcccatc aggcttggat cctgctgtgg cagtgtaatc
     2101 gtcggggaaa cttcaatagt tgttggcggt tttgcgcatc ctgcaagcat aaacagagca
     2161 actaacggga agaggatttt tttgaacatg ttcgggctct cagagactct taagcgtgtt
     2221 tggtaaaaat tcccgccatc ataacattgc caacggcgag gggaagtggg taaggcatgt
     2281 aaattcatca tgttgacgaa ataatcaccc ctggtaaaag aaacgctgat gcgaggcctg
     2341 tgtttcaatc tttaaatcag taaacttcat acgcttgacg gaaaaaccag gacgaaacct
     2401 aaatatttgt tgttaagctg caatggaaac ggtaaaagcg gctagtattt aaagggatgg
     2461 atgacatctc agcgttgtcg gaggagatat ttcatgatga tacgtgagcg gatagaagaa
     2521 aaattaaggg cggcgttcca acccgtattc ctcgaagtag tggatgaaag ctatcgtcac
     2581 aatgtcccag ccggctctga aagccatttt aaagttgtgc tggtcagcga tcgttttacg
     2641 ggtgaacgtt ttctgaatcg tcatcgaatg atttacagta ctttagcgga ggaactctct
     2701 actaccgttc atgcgctggc tctgcatact tacactatta aggagtggga agggttgcag
     2761 gacaccgtct ttgcctctcc tccctgtcgt ggagcaggaa gcatcgcgta aaaacgcatt
     2821 tgcaactgtc ggcgcttttc cagtatgttg ctaaagattt tatgaaaaac ggcctgcggg
     2881 ccgttttgtt ttgtctggat tttgcgcttt ttgcccagca ttcagacgaa aattgcccgg
     2941 gaattgtgaa aaaatacgcg acagcgcgca ataaccgttc tcgactcata aaagtgatgc
     3001 cgctataatg ccgcgtccta tttgaatgct ttcgggatga ttctggtaac agggaatgtg
     3061 attgattata agaacatccc ggttccgcga agccaacaac ctgtgcttgc ggggtaagag
     3121 ttgaccgagc actgtgattt tttgaggtaa caagatgcaa gtttcagttg aaaccactca
     3181 aggccttggc cgccgtgtaa cgattactat cgctgctgac agcatcgaga ccgctgttaa
     3241 aagcgagctg gtcaacgttg cgaaaaaagt acgtattgac ggcttccgca aaggcaaagt
     3301 gccaatgaat atcgttgctc agcgttatgg cgcgtctgta cgccaggacg ttctgggtga
     3361 cctgatgagc cgtaacttca ttgacgccat cattaaagaa aaaatcaatc cggctggcgc
     3421 accgacttat gttccgggcg aatacaagct gggtgaagac ttcacttact ctgtagagtt
     3481 tgaagtttat ccggaagttg aactgcaggg tctggaagcg atcgaagttg aaaaaccgat
     3541 cgttgaagtg accgacgctg acgttgacgg catgctggat actctgcgta aacagcaggc
     3601 gacctggaaa gaaaaagacg gcgctgttga agcagaagac cgcgtaacca tcgacttcac
     3661 cggttctgta gacggcgaag agttcgaagg cggtaaagcg tctgatttcg tactggcgat
     3721 gggccagggt cgtatgatcc cgggctttga agacggtatc aaaggccaca aagctggcga
     3781 agagttcacc atcgacgtga ccttcccgga agaataccac gcagaaaacc tgaaaggtaa
     3841 agcagcgaaa ttcgctatca acctgaagaa agttgaagag cgtgaactgc cggaactgac
     3901 tgcagaattc atcaaacgtt tcggcgttga agatggttcc gtagaaggtc tgcgcgctga
     3961 agtgcgtaaa aacatggagc gcgagctgaa gagcgccatc cgtaaccgcg ttaagtctca
     4021 ggcgatcgaa ggtctggtaa aagctaacga catcgacgta ccggctgcgc tgatcgacag
     4081 cgaaatcgac gttctgcgtc gccaggctgc acagcgtttc ggtggcaacg aaaaacaagc
     4141 tctggaactg ccgcgcgaac tgttcgaaga acaggctaaa cgccgcgtag ttgttggcct
     4201 gctgctgggc gaagttatcc gcaccaacga gctgaaagct gacgaagagc gcgtgaaagg
     4261 cctgatcgaa gagatggctt ctgcgtacga agatccgaaa gaagttatcg agttctacag
     4321 caaaaacaaa gaactgatgg acaacatgcg caatgttgct ctggaagaac aggctgttga
     4381 agctgtactg gcgaaagcga aagtgactga aaaagaaacc actttcaacg agctgatgaa
     4441 ccagcaggcg taatttacgc agcataacgc gctaaattcg cacaaaggcc cgtcaccgcc
     4501 aggtggtggg cttttttttg tcatgaattt tgcatggaac cgtgcgaaaa gcctctttcg
     4561 gtgttagcgt aacaacaaaa gattgttatg cttgaaatat ggtgatgccg tacccataac
     4621 acagggacta gctgataatc cgtccataag gttacaatcg gtacagcagg ttttttcaat
     4681 tttatccagg agacggaaat gtcatacagt ggcgaacgag ataactttgc accccatatg
     4741 gcgctggtgc cgatggtcat tgaacagacc tcacgcggtg agcgctcttt tgatatctat
     4801 tctcgtctac ttaaggaacg cgtcattttt ctgactggcc aggttgaaga ccacatggct
     4861 aacctgattg tggcgcagat gctgttcctg gaagcggaaa acccagaaaa agatatctat
     4921 ctgtacatta actccccagg cggggtgatc actgccggga tgtctatcta tgacaccatg
     4981 cagtttatca agcctgatgt cagcaccatc tgtatgggcc aggcggcctc gatgggcgct
     5041 ttcttgttga ccgcaggggc aaaaggtaaa cgtttttgcc tgccgaattc gcgcgtgatg
     5101 attcaccaac cgttgggcgg ctaccagggc caggcgaccg atatcgaaat tcatgcccgt
     5161 gaaattctga aagttaaagg gcgcatgaat gaacttatgg cgcttcatac gggtcaatca
     5221 ttagaacaga ttgaacgtga taccgagcgc gatcgcttcc tttccgcccc tgaagcggtg
     5281 gaatacggtc tggtcgattc gattctgacc catcgtaatt gatgccagag gcgcaactgt
     5341 gccgctatac ttatccaggg cggcacaacg ctgtaagcgg cttgcgcctg agaatggcat
     5401 ttgcgtcgtc gtgtgcggca caaagaacaa agaagaggtt ttgacccatg acagataaac
     5461 gcaaagatgg ctcaggcaaa ttgctgtatt gctctttttg cggcaaaagc cagcatgaag
     5521 tgcgcaagct gattgccggt ccatccgtgt atatctgcga cgaatgtgtt gatttatgta
     5581 acgacatcat tcgcgaagag attaaagaag ttgcaccgca tcgtgaacgc agtgcgctac
     5641 cgacgccgca tgaaattcgc aaccacctgg acgattacgt tatcggccag gaacaggcga
     5701 aaaaagtgct ggcggtcgcg gtatacaacc attacaaacg tctgcgcaac ggcgatacca
     5761 gcaatggtgt cgagttgggc aaaagtaaca ttctgctgat cggtccgacc ggttccggta
     5821 aaacgctgct ggctgaaacg ctggcgcgcc tgctggacgt cccgttcacc atggccgacg
     5881 caaccacgct gaccgaagcc ggttatgtgg gcgaagacgt tgaaaacatc attcagaagc
     5941 tgttgcagaa gtgcgattac gacgtacaga aagcgcagcg cgggattgtc tacatcgatg
     6001 aaatcgacaa gatttctcgt aagtcagaca acccgtccat tacccgagac gtttccggtg
     6061 aaggcgtaca gcaggcactg ttgaaactga tcgaaggtac ggtagctgct gttccaccgc
     6121 aaggtgggcg taaacatccg cagcaggaat tcttgcaggt tgatacctct aagatcctgt
     6181 ttatttgtgg cggtgcgttt gccggtctgg ataaagtgat ttcccaccgt gtagaaaccg
     6241 gctccggcat tggttttggc gcgacggtaa aagcgaagtc cgacaaagca agcgaaggcg
     6301 agctgctggc gcaggttgaa ccggaagatc tgatcaagtt tggtcttatc cctgagttta
     6361 ttggtcgtct gccggttgtc gcaacgttga atgaactgag cgaagaagct ctgattcaga
     6421 tcctcaaaga gccgaaaaac gccctgacca agcagtatca ggcgctgttt aatctggaag
     6481 gcgtggatct ggaattccgt gacgaggcgc tggatgctat cgctaagaaa gcgatggcgc
     6541 gtaaaaccgg tgcccgtggc ctgcgttcca tcgtagaagc cgcactgctc gataccatgt
     6601 acgatctgcc gtccatggaa gatgtcgaaa aagtggttat cgacgagtcg gtaattgatg
     6661 gtcaaagcaa accgttgctg atttatggta agccggaagc gcaacaggca tctggtgaat
     6721 aattaaccat tcccatacaa ttagttaacc aaaaaggggg gattttatct cccctttaat
     6781 ttttcctcta ttctcggcgt tgaatgtggg ggaaacatcc ccatatactg acgtacatgt
     6841 taatagatgg cgtgaagcac agtcgtgtca tctgattacc tggcggaaat taaactaaga
     6901 gagagctcta tgaatcctga gcgttctgaa cgcattgaaa tccccgtatt gccgctgcgc
     6961 gatgtggtgg tttatccgca catggtcatc cccttatttg tcgggcggga aaaatctatc
     7021 cgttgtctgg aagcggcgat ggaccatgat aaaaaaatta tgctggtcgc gcagaaagaa
     7081 gcttcaacgg atgagccggg tgtaaacgat cttttcaccg tcgggaccgt ggcctctata
     7141 ttgcagatgc tgaaactgcc tgacggcacc gtcaaagtgc tggtcgaggg gttacagcgc
     7201 gcgcgtattt ctgcgctctc tgacaatggc gaacactttt ctgcgaaggc ggagtatctg
     7261 gagtcgccga ccattgatga gcgggaacag gaagtgctgg tgcgtactgc aatcagccag
     7321 ttcgaaggct acatcaagct gaacaaaaaa atcccaccag aagtgctgac gtcgctgaat
     7381 agcatcgacg atccggcgcg tctggcggat accattgctg cacatatgcc gctgaaactg
     7441 gctgacaaac agtccgttct ggagatgtcc gacgttaacg aacgtctgga atatctgatg
     7501 gcaatgatgg aatcggaaat cgatctgctg caggttgaga aacgcattcg caaccgcgtt
     7561 aaaaagcaga tggagaaatc ccagcgtgag tactatctga acgagcaaat gaaagctatt
     7621 cagaaagaac tcggtgaaat ggacgacgcg ccggacgaaa acgaagccct gaagcgcaaa
     7681 atcgacgcgg cgaagatgcc gaaagaggca aaagagaaag cggaagcaga gttgcagaag
     7741 ctgaaaatga tgtctccgat gtcggcagaa gcgaccgtag tgcgtggtta tatcgactgg
     7801 atggtacagg taccgtggaa tgcgcgcagc aaggtcaaaa aagacctgcg tcaggcgcag
     7861 gaaatccttg ataccgacca ttatggtctg gagcgcgtga aagatcgcat ccttgagtac
     7921 cttgcggttc aaagccgtgt caacaaaatc aagggaccga tcctctgcct ggtagggccg
     7981 ccgggggtag gtaaaacctc tcttggtcag tccattgcca aagccaccgg gcgtaaatat
     8041 gtccgtatgg cgctgggcgg cgtgcgtgat gaagcggaaa tccgtggtca ccgccgtact
     8101 tacatcggtt ctatgccggg taaactgatc cagaaaatgg cgaaagtggg cgtgaaaaac
     8161 ccgctgttcc tgctcgatga gatcgacaaa atgtcttctg acatgcgagg cgatccggcc
     8221 tctgcactgc ttgaagtgct ggatccagag cagaacgtag cgttcagcga ccactacctg
     8281 gaagtggatt acgatctcag cgacgtgatg tttgtcgcga cgtcgaactc catgaacatt
     8341 ccggcaccgc tgctggatcg tatggaagtg attcgcctct ccggttatac cgaagatgaa
     8401 aaactgaaca tcgccaaacg tcacctgctg ccgaagcaga ttgaacgtaa tgcactgaaa
     8461 aaaggtgagc tgaccgtcga cgatagcgcc attatcggca ttattcgtta ctacacccgt
     8521 gaggcgggcg tgcgtggtct ggagcgtgaa atctccaaac tgtgccgcaa agcggttaag
     8581 cagttactgc tcgataagtc attaaaacat atcgaaatta acagcgataa cctgcatgac
     8641 tacctcggtg ttcagcgttt cgactatggt cgcgctgata acgaaaaccg tgtcggtcag
     8701 gtaaccggtc tggcgtggac ggaagtgggc ggtgacttgc tgaccattga aaccgcatgt
     8761 gttccgggta aaggcaaact gacctatacc ggttcgctcg gcgaagtgat gcaggagtct
     8821 attcaggcgg cgttaacggt ggttcgtgcg cgtgcggaaa aactggggat caaccctgat
     8881 ttttacgaaa aacgtgacat ccacgtccac gttccggaag gtgcgacgcc gaaagatggt
     8941 ccgagtgccg gtattgctat gtgcaccgcg ctggtttctt gcctgaccgg taacccggtt
     9001 cgtgccgatg tggcaatgac cggtgagatc actctgcgtg gtcaggtact gccgatcggt
     9061 ggtttgaaag aaaaactact ggcagcgcat cgcggcggga ttaaaacagt gctaattccg
     9121 ttcgaaaata aacgcgatct ggaagagatt cctgacaacg taattgccga tctggacatt
     9181 catcctgtga agcgcattga ggaagttctg actctggcgc tgcaaaatga accgtctggc
     9241 atgcaggttg tgactgcaaa atagtgacct cgcgcaaaat gcactaataa aaacagggct
     9301 ggcaggctaa ttcgggcttg ccagcctttt tttgtctcgc taagttagat ggcggatcgg
     9361 gcttgccctt attaaggggt gttgtaaggg gatggctggc ctgatataac tgctgcgcgt
     9421 tcgtaccttg aaggattcaa gtgcgatata aattataaag aggaagagaa gagtgaataa
     9481 atctcaattg atcgacaaga ttgctgcagg ggctgatatc tctaaagctg cggctggccg
     9541 tgcgttagat gctattattg cttccgtaac tgaatctctg aaagaagggg atgatgtagc
     9601 actggtaggt tttggtactt ttgccgttaa agagcgtgct gcccgtactg gccgcaaccc
     9661 gcagaccggt aaagagatca ccatcgctgc cgctaaagta ccgagcttcc gtgcaggtaa
     9721 agcactgaaa gacgcggtaa actaagcgtt gtccccagtg gggatgtgac gaagttcaag
     9781 ggcgcatcta ctgatgtgcc ttttttattt gtattcggtg actttctgtg tcttgtgggc
     9841 tgacaattgc ccccgtttct tgtcacaata ggcctttgcg cgcatcgata cgttgcgtga
     9901 ggtacacagt catctacagc ggagtgttgt tacaccatga tggacagctt acgcacggct
     9961 gcaaacagtc tcgtgctcaa gattattttc ggtatcatta tcgtgtcgtt catattgacc
    10021 ggcgtgagtg gttacctgat tggcggaggc aataactacg ccgcaaaagt gaatgaccag
    10081 gaaatcagcc gtgggcagtt cgagaacgcc tttaacagcg agcgtaatcg catgcagcaa
    10141 cagctgggcg atcaatactc cgagctggca gcgaacgaag gctatatgaa aaccctgcgt
    10201 caacaggtgc tgaatcgtct gatcgacgag gcgctgttgg atcagtacgc tcgtgagctg
    10261 aaactgggta tcagtgatga gcaggttaaa caggcgattt tcgcgacccc agccttccag
    10321 gttgacggca aatttgataa cagccgctat aacggtatcc tcaaccagat ggggatgacc
    10381 gccgatcagt acgcccaggc gctgcgtaac cagctcacta cccaacagct gattaacggt
    10441 gttgccggta ccgattttat gctgaaaggt gaaaccgacg agctggcggc actggtcgcg
    10501 caacaacgcg tggtgcgtga ggcgactatc gatgttaacg cgctggcggc gaagcagcct
    10561 gtgaccgaac aggagattgc cagctactac gaacaaaaca aaaacaattt catgacgccg
    10621 gaacaattcc gcgtgagtta catcaagctg gatgccgcaa cgatgcagca accggttagc
    10681 gatgcggata tccagagcta ctacgatcag catcaggatc aattcaccca gccgcagcgt
    10741 acccgctaca gcatcatcca gaccaaaact gaagatgaag cgaaagcggt acttgatgag
    10801 ctgaataaag gcggtgattt tgctgcatta gccaaagaaa aatctgccga tattatctct
    10861 gcccgtaacg gcggcgatat gggttggtta gaagatgcca ctatccccga cgaactgaaa
    10921 aatgctggtc tgaaagaaaa agggcaactg tctggtgtca tcaaatcttc ggtcggtttc
    10981 ctgattgtac gtctggacga cattcagcca gcgaaagtga aatcgttaga cgaagtacgt
    11041 gacgacattg cggcgaaagt gaaacacgaa aaagccctcg atgcgtacta tgcgctgcag
    11101 cagaaagtga gcgatgcggc aagcaacgac accgagtctc tggccggtgc agagcaagct
    11161 gctggcgtta aagccactca gacgggttgg ttcagcaaag ataacctgcc ggaagagttg
    11221 aacttcaagc cggttgccga cgctatcttt aacggcggtc tggtaggtga aaacggcgcg
    11281 ccgggcatca actctgacat catcaccgta gacggcgacc gcgcattcgt gctgcgcatc
    11341 agcgagcaca aaccggaagc ggtgaaaccg ttggcagatg ttcaggaaca agttaaggca
    11401 ttggttcagc acaacaaagc tcaacaacag gcgaaagtgg atgctgagaa actgctggtt
    11461 gatttgaaag ccggcaaagg tgcggaagct atgcaggctg ccggtctgaa atttggcgag
    11521 ccgaaaacct taagtcgttc cggtcgtgac ccgattagcc aggcggcgtt tgcactgcca
    11581 ctgccagcga aagacaaacc gagctacggt atggcgaccg atatgcaagg taatgtggtt
    11641 ctgctggcgc tggatgaagt gaaacaaggt tcaatgccgg aagatcagaa aaaagcgatg
    11701 gtgcagggta tcacccagaa caacgcacaa atcgtctttg aagctctgat gagtaacctg
    11761 cgtaaagagg cgaaaatcaa aattggcgat gcgctggaac agcaataatc ctgaagccgc
    11821 ctcgcaaaaa aatgcgttgc agctgtaaca agaaaaggtc gctttcgcgg ccttttccat
    11881 ttctgaacat tgccatttgt ttactgtttt cactgccgtt aaggtgattc cactgttaac
    11941 aaacaaggag aaaacagtat gaaacacgga attaaagcac tgctcattac cctgtccctg
    12001 gcctgtgccg gaatgtctca tagcgcgctg gcggcagctt ctgtggcgaa accgacaacg
    12061 gtagaaacca aagcggaagc tcctgcagca caaagtaaag cagcagtacc ggcgaaagcc
    12121 agtgacgaag aaggcacccg ggtcagcatt aataatgcca gcgcggaaga gctagcccgc
    12181 gcgatgaatg gcgttggcct gaagaaagcg caggcgattg tcagttatcg cgaagagtac
    12241 ggtccgttta aaacggtgga ggatctaaag caggtgccgg ggatgggcaa ttcgctggtg
    12301 gaacgtaatc tggcggtatt aaccctgtaa ttaatttgca tagtggcaat tttttgccag
    12361 actgaagagg tcataccagt tatgacctct gtacttataa caacaacgta aggttattgc
    12421 gctatgcaaa cacaaatcaa agttcgtgga tatcatctcg acgtttacca gcacgtcaac
    12481 aacgcccgct accttgaatt tctcgaagaa gcccgctggg atgggttgga aaatagcgac
    12541 agttttcagt ggatgacggc ccataacatc gccttcgtcg tggtcaatat caatattaac
    12601 tatcgtcgcc cagcggtatt aagtgacctg ttaactatta ccagtcagtt gcagcaatta
    12661 aacggtaaaa gcggcatctt aagccaggtc attacactgg agccggaagg gcaggtggta
    12721 gcggatgcgc ttattacgtt tgtttgtatt gatcttaaaa cgcagaaagc attagctctg
    12781 gaaggggaat tgcgcgaaaa gctggagcag atggttaagt aaacgttttg tggtgccgga
    12841 tgctcaagcc gcatccggcg acacccggaa taattacctc aacccggttt tctgcttcat
    12901 cgctgccatc accgtcggtt tatcggccag ataatgattc aaaccgttgg cgcgtaaatt
    12961 acatgccgca caatgaccgc aaccgtcgcc tttaatgccg ttatagcagg tcaacgtttc
    13021 gttacggact aaatccagtt tgccgtaata atctgccagc gcccaggttt ccgctttatc
    13081 aatccacatc agcggcgttt caaaacgaat atctttcgcc atgcccaaac tgacggcatg
    13141 gtttagtgct ttcacaaact catcgcggca atccgggtag ccggagaaat ccgtttcaca
    13201 gacgccggta attacggctt ctgcttttac ctgatacgca tatattgccg ccagcgtcag
    13261 gaacaaaata ttacgccctg ggacaaacgt attcgggatg ccatcggctt caggttcata
    13321 atcaggcacc ggaatgctgt cacgtgtcag gctgctgacc gccagctcgt tgagcagcgt
    13381 tacatcaaga accttatgcg cgcgtgctcc cagtttcagc gccagttcgc gtgccacgtc
    13441 gatttctgcg cgatgtcgct gaccgtaatc gaacgtcacg caatggactt catcatattg
    13501 ttgtaatgcc tgcaccagac aggtggtgga atcctggcct ccactgaaca caacgacagc
    13561 acgtttcata gataatcctg cttgaacaat aaaagcgtta tggtaacgcc tgcgattaac
    13621 ccggaccagc tattcattgc gacggcgcgg gaagccaggc ttcggtaaat tcaaaccagc
    13681 cgcgcggtgt cagtcgcaca ccgttcacgc cgggaggggc actaatgcga tagtgatagt
    13741 tgaacagcgg cgtcagcgtc gcgtctgcca ttaactggct aaaaaccgct ttcagggcat
    13801 taaatcggtt ttcctcatca ggcattactt gcaccgcgtc cagcgtcgat tgcagatgtg
    13861 catatgctgg agcgtcgaaa acatgtggcc atagtggatc gcaacgtagc cattgctcca
    13921 gagtatattc cggtgcttcg ccaattaatc tgtcgcccat catgaggtct gcgtgtgcca
    13981 gtagggtcgt gtcgtcccag ttttttgcgt tatgaaaaat aattgtgagt tcacagcctt
    14041 ccgctgccag tgtcgcctgt aggcgttctg ccatggtatg aagttctatc ggtaggtgat
    14101 aaaccagcgt caaggttttc ggtagtttga cttcatccgg tacttgccag tacggaatag
    14161 tccagcctgg cagtaatgca tgactggcgg tgatcaggtt ttctccgact tctaacgttt
    14221 gtaataaacc ggattgatga ataatggaga tcacttttcg cgcctgccag agggagagtc
    14281 ggggactttt gcgcaacgtt aaatagcaaa aacctaaact gatgccacta ctgacctggc
    14341 tgacccgttg cagctcctcc ggtttgccga tggtgatttg cacgggatgc cgacaactgt
    14401 ttcccaaatc tttttcgaaa agcggcggag ttatccagta ctcaaccgct ttaagcagcg
    14461 gatgacgcaa atggtaataa tcatggctct ccaggcgcac cagctccgct gtgaattgtg
    14521 ttaagcgaaa aggaccagtg ccgatcagtg ggaattgcgg atgcgccaga tggctgcaat
    14581 agctcgccag ccggtgcgca agccagtaat cggggcgatg taaaaagaag gtcagacact
    14641 gcggatgggt gacttcaata cgcttcacgc taataaataa ttgatccagt gctggcagtt
    14701 gtaacagcat caataatcgc tggtgtaagt gtgaggcttt tactgcatcg ccgttatgcc
    14761 agtgtagggt tgaacgaaga taaaagtccc agcgtaaccc gtcagtagag gtttcccagt
    14821 gatgcgctaa atcgccaatc gggcgctgag tattattatc gaagcgggtc aggccggaaa
    14881 atatctgccc ggcgagatgc tgctcggcac ggccgggcaa aaagcctggt tgtagcggtt
    14941 cgagcgggcg atagtaggga atacgcaacg tgggtgtatc gttttgccat tgtccgccca
    15001 taaacggctg taacagagtg cgcagctcac ctggggccag ttgcgccagc tccagcacat
    15061 cttgctgctt tccggtttcc agtgcctgtt ccatcatcgc attgcgtagc gattccggcg
    15121 tgaccagaaa gcgtaattgt ccgcgctttc cgcgtcctga ctgcgcctgc cactccagcc
    15181 atcccgcctc ctgtgcctga cgcaacagcg tacgaacatg gcgttcgctg caaaaacagc
    15241 gttcggccag ttcgctgacg gtgacggttt gcggctttcc ggctgaaggt tgccacagac
    15301 gttgatactg gttaagacgg ttgagcaatc gcatataaac ccggaacaat attatttaac
    15361 tattcactat tacttccgta tatatcaggt gatactcaat caccattaac cgtgtcacag
    15421 agtggagaaa gaaatggctc gtctggcagc atttgatatg gatggcactt tattgatgcc
    15481 tgaccatcat ttaggtgaga aaaccctctc tactttggcg cgactgcgtg aacgcgacat
    15541 taccctcact tttgccacgg ggcgtcatgc gctggagatg cagcatattc tcggggcgct
    15601 atcgctggat gcatatttga ttaccggcaa cggaacgcgc gtgcattctc tggaaggtga
    15661 acttttacat cgtgatgatt tacctgcgga tgtcgcggag ctggtgctgt atcagcaatg
    15721 ggatacccga gccagcatgc atatcttcaa tgacgacggt tggtttaccg ggaaagagat
    15781 ccctgcgttg ttgcaggcat ttgtctatag cggttttcgt tatcagataa tcgatgtcaa
    15841 aaaaatgcca ctcggcagcg tcaccaagat ctgcttctgt ggcgatcacg acgatcttac
    15901 acgcttgcag atccagctat acgaagcatt aggcgagcgt gcacatttgt gtttttccgc
    15961 cacggattgc ctcgaagtgc tgccggtggg ctgcaataaa ggcgctgcat tgacggtgct
    16021 gacccaacat ttaggtttat cgttgcgcga ttgcatggcc tttggtgatg cgatgaacga
    16081 tcgcgaaatg ttaggcagcg tcggtagcgg gtttattatg ggcaatgcga tgccgcaact
    16141 gcgcgcggag ctcccgcatt taccggtgat tggacattgc cgaaatcagg ctgtctctca
    16201 ctatttgacg cactggctgg actatccaca tctaccttat tcccccgaat aacgagatcc
    16261 cttccagcac cgggcaattg cccggttttt tttgcgttga atttgtcatt ttgtgccgtg
    16321 gtgtttaaac cgcacagaat aaattgtcgt gatttcacct ttaaaataga attaaaagag
    16381 aaaaaaattc tctgtggaag ggctatgtta gataaaattg accgtaagct gctggcctta
    16441 ctgcagcagg attgcaccct ctctttgcag gcactggctg aagccgttaa tctgacaacc
    16501 accccttgct ggaagcgcct gaaacggctg gaggacgacg gtatccttat cggcaaagtc
    16561 gccctgctgg atccggaaaa aataggcctc ggcctgaccg cttttgtgct gataaaaacg
    16621 caacatcaca gcagcgaatg gtattgccgc tttgtcacgg tggttaccga aatgccagaa
    16681 gtgctggggt tctggcgcat ggctggtgaa tacgattatc tgatgcgcgt ccaggttgcc
    16741 gacatgaaac gctacgacga gttttataag cgtctggtaa acagcgtgcc ggggctgtcg
    16801 gacgtcactt ccagcttcgc gatggaacag attaaataca ccacttcttt acccatcgaa
    16861 taaatatcca gaatcaggtc aggacacaac gcgtgcgatt atttgctcaa ttaagctggt
    16921 atttccgtcg ggaatggcgt cgctatctcg gggctgtcgc cttgcttgtc attatcgcga
    16981 tgctgcaact ggttccgcca aaagtggttg gtattgttgt cgatggcgtg acagaacaac
    17041 actttactac cgggcagatc ctgatgtgga tcgccaccat ggtgctgatt gccgttgtgg
    17101 tttatctcct gcgttacgtc tggcgggtat tgctgtttgg tgcgtcttat caactggctg
    17161 ttgaactgcg tgaagattat taccgtcagc taagccggca gcatcctgag ttttacctgc
    17221 gtcatcgcac gggtgatctc atggctcgtg cgaccaatga cgtcgatcgc gtcgtgtttg
    17281 ccgccggaga aggggtgctg acgctggtgg attcactggt gatgggctgc gctgtgttga
    17341 ttatgatgtc tacgcaaatt agctggcagt tgaccttatt tgccctgttg ccgatgccag
    17401 tgatggcgat catgatcaag cgcaacggcg atgccttgca tgaacgcttt aagctggcac
    17461 aggcggcgtt ttctagtctt aatgaccgca cccaggaaag cctcaccagt atccgcatga
    17521 tcaaagcctt tggtctggaa gatcgccagt cggcgttatt tgccgcggat gccgaagata
    17581 ccggcaaaaa aaacatgcgg gtggcgcgta ttgatgctcg tttcgaccca accatctata
    17641 tcgcgattgg tatggcgaac ttgctggcga ttggcggcgg tagttggatg gtggcgcagg
    17701 gcagtttaac gctgggccag ctcaccagtt ttatgatgta tttaggtctg atgatttggc
    17761 caatgctggc gctggcatgg atgtttaaca ttgtggaacg tggtagtgct gcgtacagcc
    17821 gtattcgtgc gatgctggcg gaagcgccgg tggtgaacga tggtagcgaa cccgtgccgg
    17881 aagggcgtgg cgaacttgat gtaaatattc accagttcac gtatccgcag actgaccatc
    17941 ctgcgctgga aaacgtcaat ttcgccctga aacccggtca gatgctgggt atctgcgggc
    18001 cgactggttc cggcaaaagt accctgttgt cgctcattca gcgtcatttc gacgtcagcg
    18061 agggggatat tcgctttcat gatattcctc tgacgaagtt acaactcgat agctggcgta
    18121 gccgcctggc ggtagttagc cagacaccgt tccttttttc cgacaccgta gcgaacaaca
    18181 tcgcgttggg ttgcccgaat gccacccagc aagagattga gcatgtcgcg cggttagcca
    18241 gcgtacatga cgatattttg cgtctaccgc aaggttacga tacagaagtg ggcgagcgcg
    18301 gcgtgatgct ttccggtggg caaaaacagc gtatctccat tgctcgagcg ttattagtca
    18361 acgcggaaat cctcatcctt gatgatgcgc tttcggcggt ggacggacgc acagagcacc
    18421 agatcctgca taacctgcgt cagtgggaac agggaagaac ggtaatcatc agtgcccatc
    18481 gcctttcggc actgacggaa gccagtgaaa ttattgtgat gcagcacgga catatcgccc
    18541 agcgtggcaa tcatgatgag ctggcgcaac aaagcggctg gtatcgcgat atgtatcgct
    18601 atcaacaact ggaggcggcg ctcgacgacg ctccggaaat tcgcgaggag gccgtcgatg
    18661 cgtagtttta gccaactgtg gccgactctc aagcgtctgt tagcgtacgg ttcgccgtgg
    18721 cgtaaaccgc tggggattgc ggtcctgatg atgtgggttg cggcagcggc agaagtcagt
    18781 gggccgctgc ttatcagcta ttttatcgac aatatggtag cgaaaaataa cctgccgttg
    18841 aaaatggttg cagggctggc tgcggcgtat gttgggctac aactgtttgc cgccgggcta
    18901 cattacgcgc agtcgctgct gtttaatcgg gcggcagtag gcgtagtgca acagttgcgt
    18961 accgacgtga tggatgccgc attgcgccag ccattaagcg agtttgatac ccaacccgtc
    19021 gggcaggtga tttcccgcgt cactaatgac actgaagtga tccgcgatct ctacgttacc
    19081 gtagtggcaa ctgtcctgcg tagcgccgca ctggtgggcg cgatgctggt ggcgatgttc
    19141 agcctcgact ggcgaatggc gctggtggcg ataatgattt tcccggtggt gctggtggta
    19201 atggtgatat accagcgtta cagcacgccg attgtccgcc gtgtgcgcgc ctatctagcg
    19261 gatatcaacg acggctttaa cgaaatcatc aatggcatga gcgttatcca gcagttccgt
    19321 cagcaggcgc gatttggcga acgtatgggg gaggccagtc gttcacacta tatggccagg
    19381 atgcaaaccc tgcgcctcga cggttttctg ctgcgtccgc tgctgagtct gttttcatcg
    19441 ctcattcttt gtggcttgtt gatgctgttt ggcttctccg ctagcggcac cattgaagtg
    19501 ggcgtgctgt atgcgtttat cagctatctt gggcgactta acgaaccatt aatcgaactg
    19561 accacgcaac aggcgatgct gcaacaggct gttgttgctg gtgagcgcgt gtttgaactg
    19621 atggacggac cgcgccagca atatggcaat gatgagcgcc cgttacagag tggcaccatc
    19681 gaagtcgata acgtgtcatt tgcttatcgc gatgacaatc tggtgctaaa gaacattaat
    19741 ctctctgtgc cttcgcgtaa ctttgtggca ctggtcgggc ataccggcag tggcaaaagc
    19801 accctcgcca gtttattgat gggctattac ccgctaacgg aaggtgagat tcgccttgat
    19861 ggtcgtccat taagttcgct aagtcacagc gcgctgcgcc agggcgtggc aatggtgcag
    19921 caagatccgg tggtgctggc ggataccttc ctcgccaacg tgacgctggg gcgggatatc
    19981 tccgaagaac gcgtctggca ggcgctggaa actgtgcaac tggcggagct ggcgcgtagc
    20041 atgagcgacg gtatttacac gccgctgggc gagcagggga ataatctctc agttgggcaa
    20101 aagcaactgc tggcactggc gcgcgtgctg gtcgagacgc cgcaaatcct gatccttgat
    20161 gaggcaaccg ccagcattga ctccgggact gaacaggcga ttcaacatgc tctggcggcg
    20221 gtgcgtgaac ataccacgct ggtggtgatt gctcaccgct tatcgaccat tgttgatgcc
    20281 gacaccattc tggtgcttca tcgtgggcaa gccgtggagc agggcactca ccagcaactg
    20341 ctggcggccc agggacgcta ctggcagatg tatcaactgc aacttgcggg cgaagagctg
    20401 gcagccagcg tgcgtgaaga agaatcattg agcgcctgaa tagcgcaata tttcatcgtt
    20461 ggtgcaaaaa tgtaacgcac tgtgcactgt catagtgcgt tttcattttc aatcttctta
    20521 acttcctgct ctctttctcg tttttcattt ctggcacacc gcttgcaata ccttcttcgt
    20581 gtagcagaac cattaccgaa ttctgaccgg aggggatcta tgaagctggt gaccgtgata
    20641 atcaaaccat tcaagctgga agacgttcgt gaagcgttat cttccattgg tattcagggc
    20701 ctgaccgtca ccgaagtgaa aggtttcggg cgtcagaaag ggcatgccga gctgtaccgg
    20761 ggggcggaat acagcgtcaa cttcctgcca aaagtaaaaa ttgatgtggc gattgctgat
    20821 gaccaactcg atgaagtgat cgatatcgtc agtaaggcgg cttacaccgg aaaaattggc
    20881 gacggcaaaa tcttcgtcgc tgaattgcaa cgcgtcattc gtatccgtac cggcgaagcc
    20941 gacgaagcgg cgctgtaatc tctggcacac agcaacagga acgaaaaatg aagatagcga
    21001 cgataaaaac tgggcttgct tcactggcga tgctgccggg actggtaatg gctgcacctg
    21061 cggtggccga taaagccgac aatgcgttta tgatgatttg tactgcgctg gtgctgttta
    21121 tgactattcc ggggattgcc ctgttttacg gtgggttgat tcgcggcaaa aacgtgctgt
    21181 cgatgctgac gcaggtgacg gtgacgtttg cactggtctg tattctctgg gtggtttacg
    21241 gttactcgct ggcgtttggt gagggcaaca acttcttcgg caacattaac tggttgatgc
    21301 taaaaaacat cgaactgacg gcggtgatgg gcagcattta tcagtatatc cacgtggcgt
    21361 ttcagggctc gtttgcctgc attaccgtcg gcttgatcgt tggggcgctg gcggaacgaa
    21421 tccgcttctc agctgtgttg attttcgtgg tggtatggct gacgctctct tacattccga
    21481 ttgcgcatat ggtgtggggc ggtggtttgc tggcttctca cggtgcgctg gatttcgcgg
    21541 gtggcacggt ggtgcatatt aacgccgcaa tcgccggtct ggtgggcgcg tatctgatag
    21601 gcaaacgcgt gggcttcggc aaagaggcgt ttaaaccgca caacctgcca atggtcttca
    21661 ccggtactgc cattctctat attggttggt ttggctttaa cgccgggtca gcgggcacgg
    21721 cgaatgaaat cgcggcactg gcatttgtga atactgtggt cgcaacggcg gcggcaattc
    21781 ttggctggat cttcggtgaa tgggcgctgc gtggtaagcc ttctctgctg ggagcgtgtt
    21841 ctggcgcgat tgccggtctg gtcggcgtga cgccagcgtg tggctacatt ggggttggcg
    21901 gcgcgttgat tatcggcgtg gtagctggtc tggcgggctt gtggggcgtt actatgctca
    21961 aacgcttgct gcgggtggat gatccctgcg atgtcttcgg tgtgcacggc gtttgtggca
    22021 ttgtcggctg tatcatgact gggatttttg ccgccagttc gctgggtggc gtgggcttcg
    22081 ctgaaggtgt gacgatgggc catcagttgc tggtacagct ggaaagcatc gccattacga
    22141 tcgtctggtc cggtgtggtg gcatttattg gctacaaact ggcggatctg acggttggtc
    22201 tgcgtgtacc ggaagagcag gagcgagaag ggctggatgt caacagtcac ggcgagaatg
    22261 cctataacgc gtaacaagca ctgcaaaaaa tagccggacg gttttacctc cggctatttt
    22321 tttaattgtg attacgcatc accccttcct gaacggtcga ggcaaccagc acgccgtcct
    22381 gggtataaaa ctcaccgcgc acaaagccgc gtgcgctgga cgccgaggtg ctctccacgc
    22441 tatacagcag ccattcattc aaattaaacg ggcgatggaa ccacatggaa tggtcaatgg
    22501 tggcaatctg aatccccggt tcgagaaaac cgatgccgtg cggctgtaga gctaccggca
    22561 ggaagttaag atcagaagcg taaccgagca gatactgatg aacgcgcagg tcatccggca
    22621 cgctaccatt tgcgcggatc cacacctgac gatgtggttc tgcgacgtga cctttcagtg
    22681 ggttatgaaa ctccaccgga cggacttcca gcggacgatc gcagatgaat ttatctttaa
    22741 gcactggcgg cagcaggtgc gccagcgatt gggcgatttg cgtttccgaa ggcaggccat
    22801 caggcgctgg cgcggacggc attgtttttt gatgttcgaa acccgcttct ggtgcctgga
    22861 aagaggccgt catataaaaa atcggtttgc cgttttgaat agcagcaacc cggcgggcgc
    22921 tgaagctgtt accgtcacgc agcgtttcga catcataaat aatcggcttc ttactatcgc
    22981 cagggcgaag aaagtagctg tgaaacgaat gtaccagccg ctcttcaggg acggtctctt
    23041 ttgcagcata caaggcctga cccacgacct ggccgccaaa cacctggcgt aaacctaaat
    23101 cttcactctg gccgcgaaag agtccttcct caattttttc cagatttaac aatgtcagta
    23161 aattttttag cgcctgactc atataactct ccagtaacaa agctgccgca gcaagccaaa
    23221 gtgagttgag tataacgcaa atttgctact ggtccgatgg gtgcaatggt ctgaattacg
    23281 ggctaattac aggcagaaat gcgtgatgtg tgccacactt gttgacgtta ctattttgtt
    23341 aaccactctt ccggcgagga aagttagccc gctgatgcat tgataataag gagaaatgaa
    23401 tgaaactcgt gcacatggcc agtggtttag cggttgcgat tgcgttggcg gcttgcgcag
    23461 ataaaagcgc ggatattcag acgccagccc cggctgcaaa tacgtctatt tcagcaacac
    23521 aacaacctgc tatccagcaa ccgaatgtct ccggtaccgt ctggatccgt cagaaagtcg
    23581 cactgccgcc tgatgctgtg ctgaccgtga cactttctga cgcgtcgtta gccgatgcac
    23641 cgtcaaaagt gctggcgcag aaagcggtgc gtactgaagg taaacagtca ccattcagct
    23701 ttgttctgcc atttaacccg gcagatgttc agccgaacgc gcgtattctg ttgagtgcgg
    23761 cgattaccgt gaatgacaaa ctggtattta ttaccgatac cgttcagccg gtgatcaaca
    23821 agggcggaac taaagccgac ctgacattgg tgccggtgca gcaaaccgcc gtgccggttc
    23881 aggccagcgg tggtgcaacg actaccgtac cttcgacttc accaactcag gtgaatccgt
    23941 cttcggcagt tccagctcct acgcaatatt aagcgacgtt aaccctctcc gcccggagag
    24001 ggttagtagt tccagcgata acgctgcaag tcgatttgcc cgcttcccga taccatcaca
    24061 ccttctgcca gtaatgcctg tcgctgacgc tgtaaatccg gtccggttag cgaaattgtg
    24121 ccgtggcgat taaccacccg gtgccagggt aaggtgctgc cttcagggag acgctttaac
    24181 acaccgccca cctggcgcgc ggcgcggggc gatcccgcca gtttcgccac atcaccgtaa
    24241 gtggtgacat agccttcggg aatagcggcg acgatttgcc agacgcgttg gggaaatgaa
    24301 tcttcttttt ccatcttttc ttcctgaggt aatttttcag cataatctgg aaaaacgccc
    24361 gagtgaagtc gcattgcgca agaaaccagc atctggcacg cgatgggttg caattagccg
    24421 gggcagcagt gataatgcgc ctgcgcgttg gttctcaacg ctctcaatgg gggctctgtt
    24481 ggttctcccg caacgctact ctgtttacca ggtcaggtcc ggaaggaagc agccaaggca
    24541 gatgacgcgt gtgccgggat gtagctggca gggcccccac ccatttctgc ctcccaccgt
    24601 ttcgtcaaaa aaccccaaca tggctaaact ttaaccacaa ctgacgtcgc aagaattgtc
    24661 tggctgcgca gtacgcttcg gaggtatgtc tgatgaagta tgttgatggt tttgtggttg
    24721 ccgttcctgc cgataaaaag gatgcctatc gggaaatggc cgctaaggct gcgccattgt
    24781 ttaaagagtt tggcgcgctt cgtattgtcg aatgctgggc cagcgatgta ccggatggca
    24841 aagtgaccga ttttcgtatg gcggtgaaag cggaagagaa tgaagaggtt gtctttagct
    24901 ggattgaata cccttcaaaa gaggtccgcg acgctgctac tcaaaagatg atgtcggacc
    24961 cacggatgaa agagttcggc gagtccatgc cgtttgacgg caagcgaatg atctatggcg
    25021 gattcgagtc aatcatcgac gaataggtag cgtgacgggc tgtgcggcgt gccgtcagcc
    25081 caaaaattca caaatgttgc tcggcccagc gtaagaaatc ttctttcggt aatgccttgc
    25141 tgtagagcca gccctgaccg tagtgcacgc catgctggcg tagccactct tcttgtttac
    25201 tggtttcgat tccctccgct accattttca gtttcagtgt ttttgccatt tcgatgatat
    25261 gcggcgtgac atttttatat tccagcgcat caacgaaaga tttatcgatc ttcagaatgt
    25321 cgacatccag atcctgtaaa taacttaaac ttgaatatcc cgtaccaaaa tcatcaagat
    25381 aaatttcatg gcccgcctcc cggtagcgag aaattatcgg ggcgctggtt ttcggatcgg
    25441 caaactcgcg ttcagtgagt tcaagcgcga tctgtctggg attaacctga tagtgattga
    25501 tcatttcacg cagcaattgc gggatttttt ccgaggtgag cacggtggat tcaagattga
    25561 tcgaaatatg ctgctgtgga tgctgacgca gccagtcgcc catatcttca aagacgcttc
    25621 ttataatcag tagcgtcaat ggctcagaaa ggcccgtttg ctgtgccagc ggaataaaac
    25681 tatctggtga caaccaacta ccgtctgtct gcggccagcg cgccagtgcc tcagcaccga
    25741 caattttgcc attggctaag gagacaatcg gctgatagtg cacgcaaata tcacgatttt
    25801 cgatagcatc ctgcagccga tgatgcggtg actggatacg gcgcaaaata cgcagcacaa
    25861 acatcgctgc cagcaggcca atcaccaacc cgagcggtaa ccaaataaag acttgccgat
    25921 gccagccttt ctgcaacatt ttcgttgaag cccaggtgat gatcgaaata ttcatctccg
    25981 ggaagggcag gatatcgtaa atgattccat tattttcgat atgctcaccg ggtgtttttt
    26041 gtagcctggt aataattccc tgagcaattt catcgctgct ggttatgaca acgttatggg
    26101 cattgccaat aatggcggca tcaatttgcc atgagctata gggaatgaca tcaataaagg
    26161 aagcggggtc gatcatgacg acataatgtg ccgttcccat ggcgaccatg taacggataa
    26221 tgcctaaatc gttatgcgat gttaaccaga cacgataacc atctttcgaa attttacctg
    26281 gctcggggaa ggtatcgggc ggactttcat gctccagaga cgaacactgg ggaacgttgt
    26341 tatcgatata aaccacttcc tgaatatagc gataactgta agatacccga cgcatttcca
    26401 tgagatgggc ttcgctacag gcagcgcctt gccatctttc cagctcttgc agcgcatctt
    26461 tcccttgtgt cgccacctta ttggcccgaa tagcgacgcg ggaggaatag gtatccagct
    26521 cttcaataaa cgatgtttct acctgctgat gggccagcca gatgcttaag ccgacaggca
    26581 gcaatactga aagaatcagt actcccgaaa taaggccgac cagatgtcgt gttctcacca
    26641 gcatatcctt attaacacag tgtagggaaa gtatatctga ttgtggaaaa aggctggcag
    26701 gagataagga ggtagagctg gtatttcatg aagtgatggg tggaaacgat cattgtcatt
    26761 tgaccaggcg cagagcttta cgcctggtat gtaaccctaa cttaagatgt tgattcggcg
    26821 cgagcgaggg taaaaatatc gtaaaaagac agttcacctt tacggacgat cattttttgc
    26881 agttgttcct tatgaacatc cgcgacacgc ggagagttca agatggcttc aaccagcgcg
    26941 gcaggtacaa agcgcgtgtt gtaccattcg ccgttatagc aaacgcggaa atccagcacg
    27001 tcgatatctt catagcggta gcgtgggtag acatcgaaaa agaagaaacc attaagcagg
    27061 ataaataata caaccagtag ccagacagag cctgacagcg tttcggactg caacatcacc
    27121 gccagggtgg caaaaaaagc aacgtacata ccgataaaca gccccggatg tttacggata
    27181 aaactgatac taaagcgggg tttgttgtcg cgtttttcgc gggtattcag agactcaatc
    27241 gtttcggtca gcaggcgttg tatttcggtc atcgtttgcc ttcgtggtat ctgcaacttt
    27301 ccagggaaca ccctatttta acgtgggaat agtagagggg gagtaacaga tttaccacaa
    27361 ttttgcataa cagttgcgaa aaaccgatta caatttttta attattctgg ctggattacc
    27421 gcccacgaca acgttgtccg ggacatcttt tgtgacaact gcacctgagg ctaccacgac
    27481 gttatcacca atggtcacac cagggttaat gaccgcgcgt ccgccaatcc agacgttatt
    27541 accgatggtg acgggtttcc ccagttcagc accgctatta cgtgctacag ggtcgatggg
    27601 atgtgttgcc gtgtagatat gaacgcctgg tgccaacata cagttatcac cgatgcgaat
    27661 agggcagaca tcaagcatca cgcaatcgaa gttggcgaaa aaattattac cgagaaaaat
    27721 gttatagcca tagtcacagc gaaacgttgg ctcaatataa gcctctgtca cctgaccgaa
    27781 tagatcagcg agaatttgct ggcgtaatgt gtgctcttcc gccagggaat gattgtatcg
    27841 gtgaataagc tgacgagcgc gcaggcgatc gcgagataac gtctcatctg ccgagcgata
    27901 caactcacca gcaatcatct tttctttttc tgtgctcatt actgacctcc ggttgctgaa
    27961 aatgcggcaa cacagtaaga cgaaaagtca gtgaagagaa cgttcccatt acatctgtgt
    28021 gataaagatc
//
LOCUS       DAZGVQ010000058        27727 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000058 DAZGVQ010000000
VERSION     DAZGVQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27727
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.058"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(68..298)
                     /locus_tag="ACHLAG_002355"
     CDS             complement(68..298)
                     /locus_tag="ACHLAG_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655571.1"
                     /translation="MRTAESPGRKITARPANHHAHISIAARKAERAHICELLHDLVNT
                     AREKHTSPEETCSSIERLIAILANPDELKGCA"
     gene            complement(513..638)
                     /locus_tag="ACHLAG_002356"
     CDS             complement(513..638)
                     /locus_tag="ACHLAG_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655572.1"
                     /translation="MSVFLSWVILIISVLMTIGIMRIIHTLKDIELFFTGDKRRK"
     gene            complement(635..874)
                     /locus_tag="ACHLAG_002357"
     CDS             complement(635..874)
                     /locus_tag="ACHLAG_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFP0655573.1"
                     /translation="MTRNYKEIVNQIIKAYSHRIAKSPEFIRIMETRSLMQGLNGLAL
                     AALAANDIDATIEINNLLLDIEKGGNIEPYNLEAA"
     gene            complement(893..1108)
                     /locus_tag="ACHLAG_002358"
     CDS             complement(893..1108)
                     /locus_tag="ACHLAG_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655574.1"
                     /translation="METYKIKNIEHHDGFFSTEHVACVVRIFNEVDVILKQNIKLEII
                     AKSEADAIEIKKAIERQIMPLTSIIAF"
     gene            complement(1122..1445)
                     /locus_tag="ACHLAG_002359"
     CDS             complement(1122..1445)
                     /locus_tag="ACHLAG_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFP0655575.1"
                     /translation="MKQQRNARINNNTERHVARQHFRKNSATATTEIHTTPDGHPVKQ
                     TGKHTWAIGDTGIVIHKSFRSPVTGRFNFTLTRGDDYFGQDFTFFEALRTVDRLMTGR
                     NFHAK"
     gene            complement(1471..1665)
                     /locus_tag="ACHLAG_002360"
     CDS             complement(1471..1665)
                     /locus_tag="ACHLAG_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655576.1"
                     /translation="MTDSTQTKDLYSDLRFIIIQQQSGRMDPTRASKAWKEALTDQGI
                     DVISEVMAEVIFRLTRDESL"
     gene            complement(1717..2241)
                     /locus_tag="ACHLAG_002361"
     CDS             complement(1717..2241)
                     /locus_tag="ACHLAG_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0655577.1"
                     /translation="MRKSIADKELSDTQALQENDSITESVGAANDDEHQEEKKKRTYK
                     KGATLRLDGPVAGLCSLEKGAAYIGLTKAALRVALQRNQMPGHKTRSNPEDENSDGVW
                     WFNAREWDKLADELPECEPPEWHNWKSYWTYDRQKRKFSPANKEDCQTINGKRVYTGR
                     KSKLEQHQYSEGNQ"
     gene            2345..2686
                     /locus_tag="ACHLAG_002362"
     CDS             2345..2686
                     /locus_tag="ACHLAG_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0655578.1"
                     /translation="MVNMSRDYAKKLKAIRNAENLTQSQFSQVTGVSVGTIGNYESGY
                     KPARIEVVEKVIQVERFEKYTMWILHDKTFPAAGQVAPALSLDGSIQSEGDQVSIGIT
                     QKSPRSRRNAG"
     gene            2756..3748
                     /locus_tag="ACHLAG_002363"
     CDS             2756..3748
                     /locus_tag="ACHLAG_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0655579.1"
                     /translation="MSIKQLKDGRYKVDVRPQGTEGKRIRKIFTLKSKAQEFERYVLQ
                     NFHDKPWQAKPADQRRLSELIDAWWVLDGRNQPYGDTYRVRLEKINREMRDPRASEMT
                     RKFMLGYRSDKLQAGLMPSSVNRDLCALSAMFTSLIDAEVFHNENPVRGIRKLKVRNT
                     EMAFLSDDEIDRLLERLEGDARRVAVLCLSTGARWGEAAGLRGEHIVGNRVMFFNTKN
                     GKSRAVPVSDTVLSLIKTRKTGLLYQADYIRFRDILQEVKPDLPKGQATHVMRHTFAT
                     HFMMNGGNIVTLQRILGHATIQQTMTYAHFSPDFLQDAISFNPLADSVHKLSID"
     gene            3799..4329
                     /locus_tag="ACHLAG_002364"
     CDS             3799..4329
                     /locus_tag="ACHLAG_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 family protein"
                     /protein_id="HFP0655580.1"
                     /translation="MSKSSRGVRCVKSPEGVLPVSQTQQGEGQIWRREVSSRYGQYPK
                     AQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQTLNKTRPAMVDVIPTDGI
                     VPLYINPQGVAKLLRNETLTSLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQM
                     EPGAAWQWLPITWQPL"
     gene            4326..4949
                     /gene="yfaT"
                     /locus_tag="ACHLAG_002365"
                     /pseudo
     CDS             4326..4949
                     /gene="yfaT"
                     /locus_tag="ACHLAG_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
     gene            4973..9487
                     /locus_tag="ACHLAG_002366"
     CDS             4973..9487
                     /locus_tag="ACHLAG_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFP0655581.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRIGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDTRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQAAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKMAQQSNIPVTVERQLYRLIPGEEEMSFILQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            9488..11137
                     /gene="yfaQ"
                     /locus_tag="ACHLAG_002367"
     CDS             9488..11137
                     /gene="yfaQ"
                     /locus_tag="ACHLAG_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFP0655582.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKASTLPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWATGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            11142..11918
                     /gene="yfaP"
                     /locus_tag="ACHLAG_002368"
     CDS             11142..11918
                     /gene="yfaP"
                     /locus_tag="ACHLAG_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFP0655583.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            12193..15042
                     /gene="rcsC"
                     /locus_tag="ACHLAG_002369"
     CDS             12193..15042
                     /gene="rcsC"
                     /locus_tag="ACHLAG_002369"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFP0655584.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(15242..15892)
                     /gene="rcsB"
                     /locus_tag="ACHLAG_002370"
     CDS             complement(15242..15892)
                     /gene="rcsB"
                     /locus_tag="ACHLAG_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFP0655585.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(15909..18581)
                     /gene="rcsD"
                     /locus_tag="ACHLAG_002371"
     CDS             complement(15909..18581)
                     /gene="rcsD"
                     /locus_tag="ACHLAG_002371"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFP0655586.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            19320..20423
                     /gene="ompC"
                     /locus_tag="ACHLAG_002372"
     CDS             19320..20423
                     /gene="ompC"
                     /locus_tag="ACHLAG_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP0655587.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSARGEGMTNNGRDALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDAQNTAAYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIGTRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            20535..21590
                     /gene="apbE"
                     /locus_tag="ACHLAG_002373"
     CDS             20535..21590
                     /gene="apbE"
                     /locus_tag="ACHLAG_002373"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFP0655588.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPKKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            21664..22728
                     /gene="ada"
                     /locus_tag="ACHLAG_002374"
     CDS             21664..22728
                     /gene="ada"
                     /locus_tag="ACHLAG_002374"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFP0655589.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFHPCKRCQPEKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            22728..23378
                     /gene="alkB"
                     /locus_tag="ACHLAG_002375"
     CDS             22728..23378
                     /gene="alkB"
                     /locus_tag="ACHLAG_002375"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFP0655590.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINNVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            23454..25097
                     /gene="yojI"
                     /locus_tag="ACHLAG_002376"
     CDS             23454..25097
                     /gene="yojI"
                     /locus_tag="ACHLAG_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFP0655591.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            25315..26961
                     /gene="mqo"
                     /locus_tag="ACHLAG_002377"
     CDS             25315..26961
                     /gene="mqo"
                     /locus_tag="ACHLAG_002377"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFP0655592.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSAVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(27110..27619)
                     /gene="eco"
                     /locus_tag="ACHLAG_002378"
     CDS             complement(27110..27619)
                     /gene="eco"
                     /locus_tag="ACHLAG_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFP0655593.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
BASE COUNT         6657 a         6743 c         7348 g         6979 t
ORIGIN      
        1 ccaatcgcga atatggctgc gtccggtccg ttacccagtg tttcggtgtc aatcatgaga
       61 tggttcatta tgcgcacccc ttcagttcgt ctgggttggc gagtattgca atcagtcgtt
      121 caatggatga acaggtttcc tccggggagg tgtgcttctc tctcgctgtg ttaaccaggt
      181 catgcagcaa ttcgcagata tgcgcacgct cggcttttct tgcggcaatg ctgatatgtg
      241 catgatggtt agctggtctg gcggttattt ttctgccggg tgattcggct gttctcattt
      301 gttgactcat gaaaaaagac aaagggaatc cgaaggcatg aatgccttaa ttaattgttc
      361 gtgtgttttc tggtgttatt ccgcgcctgc tttcggcttc agattatccc cagattcttc
      421 agggctaacc agataagcgc actcacctga aatatcaccc atacagtggc gatggtgagt
      481 attgctttta cattcttcat aaggttttcg ctttatttac ggcgtttatc gccggtaaag
      541 aataattcga tatccttcag tgtgtggata attcgcataa tgccgattgt cataagcacg
      601 gagataatca gaattaccca cgaaagaaat acgctcatgc ggcctccaga ttgtagggtt
      661 ctatgtttcc gcctttttca atgtctaata acaggttgtt tatttcgata gtggcatcaa
      721 tatcgttagc tgccaatgcg gccagtgcca acccgttcag tccttgcatt aatgagcgcg
      781 tctccattat gcgtatgaac tcgggtgatt ttgcgatgcg gtggctgtaa gccttaatga
      841 tctgattgac gatttctttg taatttctgg tcatggttat ttttcctgtt gcttaaaatg
      901 caatgatact tgtcaacggc attatctggc gttcaatagc tttttttatt tcaatggcgt
      961 cggcttcgct ttttgcgatg atttcaagct ttatgttttg cttcaaaatc acatctactt
     1021 cattgaaaat cctgacaaca caagcaacat gttcagtgct gaaaaaaccg tcatggtgtt
     1081 caatattttt aattttgtat gtttccatgt tttcatctgt atcatttagc gtgaaaatta
     1141 cgaccagtca ttaaccgatc gaccgttctt aacgcttcaa aaaatgtgaa gtcctgtccg
     1201 aagtaatcat ctccgcgagt cagggtaaaa ttaaatctgc cagtaacagg gctacgaaaa
     1261 cttttgtgaa taacgatgcc agtgtcgcca atcgcccagg tgtgctttcc ggtttgtttt
     1321 accggatgac cgtctggtgt ggtatggatt tcagtggttg cggtcgcact atttttacgg
     1381 aagtgctggc gtgcaacgtg gcgttcggtg ttgttattga tgcgtgcatt tcgttgttgc
     1441 ttcattcttc actcggtcct tatgcgatga ttacagtgat tcgtctcttg tcagcctaaa
     1501 aatcacttca gccatgactt cgctaattac atcgatcccc tgatcggtaa gggcttcttt
     1561 ccaggccttg cttgcacgtg ttggatccat cctgcctgat tgctgttgta tgatgatgaa
     1621 gcgtaaatcg ctgtataagt cttttgtttg agtggagtca gtcatgtcaa cacattcaaa
     1681 tcccgatgct cttaatgatc ccggtttgtt ttgatgtcac tggttgcctt cgctgtattg
     1741 gtgttgctcc agttttgact ttcttcctgt gtaaacccgc tttccattga tggtctggca
     1801 gtcttctttg tttgccgggg aaaactttct tttctgtcgg tcgtacgtcc agtagctttt
     1861 ccagttgtgc cattctggcg gctcgcattc tggtagttcg tcagccagct tgtcccactc
     1921 tctggcgtta aaccaccata cgccatccga attttcatct tcagggtttg aacgggtctt
     1981 gtgtccaggc atctgatttc gctgaagcgc cacgcgcaat gcagcttttg tcaacccgat
     2041 ataggctgcc cctttttcaa gtgagcaaag tccggctacc gggccatcca gtcgcaaggt
     2101 tgcccctttt ttgtaggtgc gttttttctt ttcctcttgg tgttcatcgt cgtttgccgc
     2161 gccaacggat tcggtgatgc tgtcgttttc ctgaagggct tgtgtgtcag ataactcttt
     2221 atcggcaatt gacttccgca tttgctatcc tcttgcgttg gtgcgcttgt cgcgcatcat
     2281 gaaggtttgt tttgatttgt ttacttcacc ctgtggtgaa gttcgaatag aggatactca
     2341 tcgtatggtg aacatgtcaa gagattacgc caaaaaacta aaggcaatca gaaacgcgga
     2401 aaaccttaca caatctcagt tttcgcaagt aacgggagtt tctgtaggaa ctataggaaa
     2461 ttatgagtct ggatataaac ctgcgcgaat tgaggttgta gaaaaagtaa tccaagtcga
     2521 gcgattcgaa aaatacacta tgtggatact tcacgacaaa acatttcccg ctgccgggca
     2581 ggttgccccg gctctctctc ttgatggctc cattcagtcg gagggcgatc aggtttcaat
     2641 cggcattacc caaaaatcac cccgatctcg tcgcaatgct ggctaacgct gtacatcgag
     2701 cgggtttcat cccgtagaga gtgtacggct gattcatccg ttggaggggc taatcatgtc
     2761 gattaagcaa ctcaaagacg ggcgttataa ggttgatgta agaccgcaag ggacggaagg
     2821 aaagcggatc cgtaagattt ttaccctgaa gtcaaaggca caggagtttg aaaggtatgt
     2881 tttgcagaat ttccacgata aaccgtggca ggccaagcca gctgatcagc gtcggttatc
     2941 cgaactgata gatgcctggt gggtgcttga tggccggaat cagccttacg gggatactta
     3001 cagggtcagg ctggaaaaaa taaaccgtga gatgagggac ccccgcgcca gtgagatgac
     3061 gcgaaagttt atgctcgggt atcgttcaga caaattacag gccggattaa tgccctccag
     3121 tgttaatcgt gatttgtgcg ccttgtctgc aatgtttacc tcgctgattg atgcggaggt
     3181 tttccataac gaaaacccgg tgcgtggaat acgtaagctc aaggtcagga atacagaaat
     3241 ggcctttctt tcagatgatg agattgatcg gctgcttgaa cgactcgagg gggatgcgcg
     3301 tcgtgttgct gttctgtgtc tttccactgg cgcaaggtgg ggggaagcag ctggcttacg
     3361 tggtgagcat atcgttggta accgcgtaat gttctttaac acaaagaatg gaaaatcccg
     3421 cgcggttcct gtttctgata cggtgctttc gttgataaaa acccgaaaaa ccgggctatt
     3481 gtaccaggct gattacatcc gttttcggga tatccttcag gaggtgaaac cggatttacc
     3541 caaaggtcag gcgactcacg ttatgcgtca cacgttcgcc acgcatttca tgatgaacgg
     3601 cggaaatatc gttacgttac agcgaatcct tgggcacgcc acgattcaac aaacgatgac
     3661 gtatgcgcat ttttcaccgg actttttaca ggatgcgatt agctttaatc cgctggctga
     3721 tagtgtccat aaactgtcca ttgactagtt gtaatttgcg ttaatttgct ccccttgttt
     3781 tgatttagtc attgaaaaat aagtaaatca tcaaggggag taaggtgtgt taaatctcca
     3841 gaaggtgttt tgccggtaag ccagactcag cagggcgaag ggcaaatctg gcgtcgcgaa
     3901 gtgagttccc gatacggcca gtatccgaaa gcgcaggcgg cgcaacccga tcaattaatg
     3961 tcggattatt ttttccgcgt gtcgctggcg atgcaaaaca aaacgctgct tttctccctc
     4021 gatgacacgc tggttaataa cgcgctgcaa acactgaata aaacccgccc ggcaatggtg
     4081 gatgtaatac ccactgatgg catcgttccg ctctatatca atccacaagg cgtggcgaaa
     4141 ctgctgcgta acgaaacgtt gaccagtctg ccgaagaatc tcgaaccggt tttttataac
     4201 gccgcacaaa ctttattaat gccgaagctg gacgctttat ctcaacaacc gcgttatgtc
     4261 atgaagctgg cccagatgga acccggtgcc gcctggcagt ggttacccat aacctggcag
     4321 ccgctatgag gcacgggctg ctggcgctga tttgctggct gtgttgtgtt gttgcccata
     4381 gcgaaatgct gaatgtcgaa caatccgggc tgtttcgcgc ctggtttgta cgcattgcac
     4441 aagaacagct ccgccagggg ccaagtccac gctggtatca gcaggattgt gcgggcctgg
     4501 tgcgatttgc ggcgaacgag acgctgaaag ttcacgatag taaatggcta aaaagtaacg
     4561 gtttctctag ccagtatttg ccgccagaga tgacgctaac acctggacag cgtcaactgg
     4621 cgcaaaactg gaatcagggg aacgggaaaa ccggccccta cgtgaccgcg attaatttga
     4681 ttcagtacaa cagccagttt attggctagg acataaacca ggcgctgcct ggcgatatga
     4741 tttttttcga tcagggcgat gcccagcact taatggtctg gatggggcgt tacgtcatct
     4801 accacaccgg aagcgccacg aaaactgaca acggaatgcg cgcagtcagt ctgcaacaac
     4861 ttatgacatg gaaggacacc cgatggatac ccaacgattc caatcccaat ttcattggca
     4921 tttatcgttt aaattttctg gcgcgatagc agcgtgcttg tccctgtctc ttgtgggaac
     4981 agggcttgct aatgctgatg attcgcttcc ttccagtaac tatgcgccgc ccgccggggg
     5041 aacattcttt ttgcttgctg acagcagttt tagcagcagt gaagaggcga aagtgcgact
     5101 ggaagcgccg gggcgtgatt atcggcgcta tcagatggaa gagtacggcg gcgtggacgt
     5161 tcgcctgtat cgtattcctg acccgatggc atttttgcgc cagcagaaaa acctgcatcg
     5221 cattgtggtg caaccgcaat atctgggcga cgggctgaac aatacgctga cctggctgtg
     5281 ggataactgg tacggcaaat ctcgccgcgt aatgcagcgt actttctctt ctcagtcacg
     5341 gcagaatgtg actcaggcat tacccgaatt acatctcggc aatgccatta ttaaaccttc
     5401 ccgttatgta cagaacaacc agttttcccc gctgaaaaaa tatcccttgg tggaacagtt
     5461 ccgttatcca ctatggcagg ctaaaccggt cgagccgcag caaggggtaa aactggaagg
     5521 cgcatccagc aatttcatct cgccgcagcc gggtaacatt tatattcctc tcggccaaca
     5581 agagccggga ctgtacctcg tcgaggcgat ggttggtggg tatcgggcga cgacggtggt
     5641 gtttgtttcc gataccgtgg cgcttagcaa agtgtcaggc aacgagcttc tggtgtggac
     5701 cgcgggtaaa aaacagggtg aagcgaagcc cggctcagag atcttgtgga ctgacggtct
     5761 tggcgtgatg acccgcggtg tgaccgatga cagcggtacc ttgcagttac aacatatatc
     5821 gccagaacgc tcatacattc tgggtaagga tgctgaaggc ggcgtttttg tctccgagaa
     5881 cttcttttac gaaagtgaaa tctacaacac ccgcttgtat atctttaccg atcgcccgct
     5941 atatcgcgca ggcgatcgtg tcgatgttaa agtgatgggc cgcgagttcc acgatccgtt
     6001 gcattcatcc cccatcgtca gcgccccggc gaagctttcg gtgctggacg ccaacggcag
     6061 tctgttgcaa accgtcaatg tcacgctgga tgcgcgcaat ggcgggcagg gaagtttccg
     6121 cctgccagaa aatgccgttg ccggaggtta tgagttacgt cttgcttacc gcaatcaggt
     6181 ctatagcagc agttttcgcg tggcaaacta catcaagcca catttcgaga ttggtttagc
     6241 tctcgacaaa aaagagttca aaactggcga agcggtcagc ggcaaactgc aactgctcta
     6301 cccggatggc gagccggtaa aaaatgcccg cgtgcagtta agtttgcgcg ctcagcaatt
     6361 atcaatggtc ggtaacgatt tgcgttatgc cggacgtttc cccgtgtcgc tggaaggcag
     6421 cgaaacggtg tccgacgcca gcggtcatgt ggcgttaaat ctccccgccg ccgataaacc
     6481 gagccgttat ttgttaaccg tctccgccag tgacggcgcg gcgtatcgtg tcaccaccac
     6541 caaagagatc ctcattgaac gcggtctggc gcattactca ttaagtactg ccgcacaata
     6601 cagtaatagc ggcgagtcgg ttgtgttccg ttatgccgcg ctggaatctt caaaacaggt
     6661 tcctgttacg tatgaatggt tgcgtctcga agaccgcacg agccatagcg gagagctacc
     6721 gtcaggcggc aaatccttta ccgtcaattt cgctaaacct ggcaactaca atctgacatt
     6781 acgcgataaa gacggcttaa ttctcgctgg gttaagtcat gccgtcagcg gtaagggcag
     6841 cacggcgcat actggtacgg tagatatcgt ggcggataaa acgctgtacc agccaggcga
     6901 aactgcgaag atgctgatta cctttccgga gccaattgat gaagcattat tgacgctgga
     6961 acgcgatcgc gtggaacagc agtcgctgct ttcgcatccg gcaaactggc tgacgttaca
     7021 acgtttaaac gatacccagt atgaagcccg ggttccagtg agcaattcct ttgcgcctaa
     7081 catcactttt tcggtgctgt atacccgtaa tggtcagtac agttttcaga acgccgggat
     7141 caaagttgcc gttcctcagc ttgatatccg ggtgaaaacg gacaaaaccc attaccagcc
     7201 tggtgaactg gtcaatgtcg aattaacctc gtcgctgaaa ggtaaacctg tttctgcgca
     7261 gctaacggta ggcgtggtcg atgaaatgat ctacgcgctg caaccagaaa tcgcgccgaa
     7321 tatcggcaaa tttttctatc cgctggggcg taacaatgtg cgtaccagct ccagtctgtc
     7381 gtttatcagc tacgaccagg cgctctccag cgagccggtt gcgcctggcg caactaaccg
     7441 cagcgagcgg cgagtaaaaa tgcttgaacg tccacggcgt gaagaggtgg ataccgcggc
     7501 atggatgccg tcactcacaa ccgataaaca aggcaaagcg tatttcacgt tcctgatgcc
     7561 tgattcgtta acccgctggc gtatcaccgc gcgtgggatg aacggcgacg ggctggtcgg
     7621 gcaggggcgt gcttatctgc gttcggaaaa aaatctctac atgaagtgga gtatgccaac
     7681 ggtgtatcgc ataggcgaca aaccggcagc aggactgttt atcttcagtc agcaggataa
     7741 cgaaccggtg gcgctggtga cgaaatttgc aggcgctgag atgcgccaga cgctgacgct
     7801 gcacaaaggg gcgaattata tttcgctgac gcagaatatt cagcaatctg gcttgttaag
     7861 tgcagaactg caacaaaatg ggcaagtgca ggacagcatt agcacaaaac tgtcttttgt
     7921 ggacaacagc tggcccgttg aacagcagaa aaatgtcatg ctcggcggtg gcgagaacgc
     7981 gctgatgttg cccgagcagg cgagcaatat ccggctacaa agtagtgaaa cgccgcagga
     8041 gattttccgc aacaatcttg atgcgttagt cgatgaaccg tggggtgggg tgatcaacac
     8101 cggtagccgt ctgatcccgc tcagtctcgc ctggcgttcg cttgccgatc atcaaagtgc
     8161 cgccgctaac gacattcgtc agatgattca ggataaccgt ctgcggctga tgcaactggc
     8221 ggggcccgga gcgcgcttta cctggtgggg tgaagatggc aatggtgacg ccttccttac
     8281 ggcatgggca tggtacgccg actggcaggc cagccaggcg ctcggcgtaa cgcaacaacc
     8341 ggaatactgg cagcatatgc tcgacagtta tgccgagcag gcagataaca tgccgttatt
     8401 gcatcgggcg ctggtgctgg cgtgggcaca ggagatgaat ctgccgtgca aaacgttgtt
     8461 gaaagggttg gatgaagcta tcgcccggcg cggaactaaa gatgaagatt tctctgagga
     8521 agacacccgc gatatcaatg acagcctgat cctcgataca ccggaatctc cactggcaga
     8581 tgcggtggca aacgtcttaa ccatgacgtt gctgaaaaaa gcgcagttga agtccacggt
     8641 gatgccacag gttcagcaat atgcgtggga taaagcggca aacagcaatc agccgctggc
     8701 gcacacggtt gtgctgctta atagcggtgg cgacgctacc caggcggctg ctattttaag
     8761 tggtttgacc gctgagcaat ccactattga gcgcgcgctg gccatgaact ggctggcgaa
     8821 atatatggcg acgatgcctc cagttgtttt gcctgcgcct gcgggcgcat gggctaaaca
     8881 taagttaact ggagggggcg aagactggcg ttgggttggt cagggcgtgc cggacattct
     8941 ctcttttggt gacgaattat cgccgcaaaa tgtgcaggtc cgctggcgtg agccggcaaa
     9001 aatggctcaa caaagtaaca ttccggtgac cgttgaacgc cagttgtatc ggcttatccc
     9061 tggtgaagaa gagatgagct ttattctgca accggtgacc agcaatgaga ttgacagcga
     9121 tgcgctgtat ctcgatgaaa ttacgcttac cagcgagcag gatgcagttc tgcgctatgg
     9181 tcaggtggaa gtaccgctcc cgccgggagc cgacgttgag cgcacaacat ggggcatttc
     9241 agtcaataaa cccaacgccg cgaaacagca ggggcaattg ctggaaaaag cgcgtaatga
     9301 aatgggcgaa ctggcctata tggtgccggt gaaagaactg acgggaacgg tcactttccg
     9361 ccatttgctg cgcttctcgc aaaaagggca attcgttctg cctcctgctc gttatgtgcg
     9421 ttcctatgca cctgcgcagc aaagtgttgc ggcagggagt gaatggaccg ggatgcaggt
     9481 gaaataagtg aactggcgca gaatcgtctg gctattggcg ttggttactc tgccaacgct
     9541 tgcggaggag acgccgttac aactggtgct gcgcggtgcg cagcacgatc aactgtatca
     9601 gctgtcgtca tcaggcgtga ccaaagcctc gacattacct gactcgctca ccacgccgtt
     9661 agggagtttg tggaaactgt atgtttacgc ctggctggag gatacccatc aaccggagca
     9721 gccctaccag tgtcgcggaa actccccgga agaggtttat tgttgtcagg ctggggagag
     9781 cataacgcgc gataccgcgc tggtacgttc ctgtgggctc tattttgcgc cgcagcggtt
     9841 gcatattggc gcagatgtgt gggggcaata ctggcaacag cgtcaggcac ctgcgtggtt
     9901 agcgtctctt actacgctga agccagaaac ctcggtaacg gttaaatcgc tgcttgattc
     9961 attagcgacg ttacctgcgc aaaacaaagc tcaggaagtc ttactggatg tggtgctgga
    10021 cgaggcgaaa atcggagtcg catccatgct cggtagtcgg gtacgggtga aaacctggag
    10081 ctggtttgcc gatgataaac aggaaatccg tcagggtggt tttgccggct ggttaaccga
    10141 tggtacaccg ctgtgggcta ctggcagtgg caccagcaaa acggtactaa cccgttatgc
    10201 gacggtgctt aatcgggttt tgccagtgcc gacgcaggtt gccagtgggc aatgtgtaga
    10261 ggtggagctg tttgcccgct atccgctgaa gaaaatcaca gcggaaaaga gtacgacggc
    10321 agtaaaaccc ggcgtactta atgggcgcta tcgtgtgacg tttaccaatg gtaaccacat
    10381 tacgtttgtc agccacggtg agacgacctt actgagcgaa aagggcaaac tgaaattgca
    10441 gtcgcatctc gatcgtgaag agtacgttgc cagagttctg gatcgcgagg cgaaaagtac
    10501 gccccctgag gcagcgaaag ccatgaccgt ggcgattcgc acttttttac agcaaaacgc
    10561 caatcgggaa ggcgattgcc tgaccatccc cgacagcagc gccacgcagc gtgtttctgc
    10621 ttcgccagcg accacggggg cgcgaacgat gaccgcctgg acgcaggatc tcatctatgc
    10681 aggcgatccg gttcattatc atggcagtcg cgctaccgaa gggacacttt cctggcggca
    10741 agccacggcc caggcggggc agggggagcg ttacgaccag atactcgcgt ttgcttatcc
    10801 cgacaacagc cttagccgct ggggagctcc acgctcaacc tgccagttat tacccaaagc
    10861 gaaagcctgg ctggcgaaaa aaatgccgca gtggcggcgt atattacaag gcgagacggg
    10921 gtacaacgaa ccagacgtgt ttgcggtctg tcgtttagtc tcaggtttcc cctataccga
    10981 tcggcagcag aaacggctgt ttattcgtaa cttctttacg cttcaggatc ggctggattt
    11041 aacccatgag tatctgcacc tggcctttga tggttatccc accgggcttg atgagaacta
    11101 tatcgaaacg ctgacccgac aattattaat ggactaatgc tatgcgaaag atttttcttc
    11161 cgttgttact ggtggcactt tcgcctgtcg ctcacagtga aggtgtgcag gaagtcgaga
    11221 ttgacgcacc gctctctggc tggcatccgg cagagggcga agatgccagt ttttcacaat
    11281 cgatcaatta cccggcgtcg tccgtcaaca tggcagatga tcaaaatatc tcagcgcaga
    11341 tccgcggcaa aataaagaat tatgctgcgg cgggtaaagt tcagcagggc cggctggtgg
    11401 tcaacggtgc cagtatgccg cagagagttg aatccgatgg ttcatttgca cgcccttata
    11461 ttttcactga aggcagcaac agcgtgcagg tcatcagccc ggatgggcaa agccgacaaa
    11521 aaatgcagtt ttactcaacg ccgggtacag gaacgattcg tgcacgttta cggctggttc
    11581 tctcgtggga tacggacaat accgatctcg atctccatgt tgtcacgccc gatggcgaac
    11641 acgcctggta cggtaacacc gtgctgaaaa acagtggtgc actggatatg gatgtcacga
    11701 cggggtacgg acccgagatt ttcgccatgc cagcaccagt tcacgggcgc tatcaggtgt
    11761 atatcaacta ctatggtggg cgcagcgaaa cggaattgac gaccgcccaa ctgacgctga
    11821 tcaccgatga aggatcggtc aatgagaaac aggaaacatt tattgtaccg atgcgtaatg
    11881 ctggcgagct gacgctggtg aaaagttttg actggtgatc catcccttca cacaacctgg
    11941 ctctctcttt tttcagataa gactgctggg cgatctgccg acgccaggat ataaccatta
    12001 acaagcgtcg gcagattggt catttcttat ttttcgtact gagaactgtc catctactcc
    12061 ggctttcctg gttaaggcaa tggtttctaa gcgatacgca ttattgcaat ggtgacagaa
    12121 cattgcggta acacgctttt accgctacct taaccacact ccatcggtca cctgaggcgg
    12181 agcttcgccc ctttgaaata ccttgcttct tttcgtacaa ccctgaaagc ctcgcgctac
    12241 atgttcagag cattggcgtt agtgctctgg ctgttgattg ctttttcatc cgttttttac
    12301 atcgttaatg cgttacatca gcgagaatcg gaaattcgtc aggaatttaa tctgagttcc
    12361 gatcaggctc agcgctttat tcaacgcacc tctgatgtga tgaaagagct gaagtacatc
    12421 gccgaaaatc gcttatcggc agaaaacggt gtgctttccc cgcgtggacg agaaacgcag
    12481 gcggatgtgc ctgcgtttga accgctgttt gccgactccg attgttccgc aatgagtaac
    12541 acctggcgag gttctctgga gtcattggcg tggtttatgc gctactggcg cgataatttt
    12601 tctgcggctt acgatctcaa ccgggtattt ttaatcggca gtgataacct ctgcatggcc
    12661 aatttcggtc tgcgtgatat gccagtggaa cgcgataccg cgttgaaagc tttgcatgaa
    12721 cgcatcaata aatatcgaaa tgcaccacaa gatgatagcg gcagtaacct ctactggatc
    12781 agcgaaggtc cgcgccctgg cgtcgggtat ttttacgcgt tgacgccagt ttatctggcg
    12841 aaccggttgc aggcgctttt gggtgtcgag cagaccatcc ggatggagaa ctttttctta
    12901 ccgggtacgt tgccgatggg ggttaccatt cttgatgaaa atggtcatac cctgatttcg
    12961 cttaccggac cagaaagtaa aattaagggc gatcctcgct ggatgcagga acgctcctgg
    13021 tttggctata cggaagggtt ccgggagctg gtgctgaaga aaaatctgcc accctcatcg
    13081 ctaagcatcg tgtattcggt gccggttgat aaggtgctgg aacgcattcg catgttgatc
    13141 cttaacgcaa ttttgctgaa tgtgcttgcc ggagctgcat tgtttactct cgcgcggatg
    13201 tacgagcgac gtattttcat tccggcggaa agcgacgccc tgcgactgga agaacatgag
    13261 cagttcaacc gcaagattgt cgcctccgcg ccagtgggta tctgcatttt gcgtaccgct
    13321 gatggcgtca atattttaag taacgaactg gcgcatacct atctcaatat gcttacgcat
    13381 gaggaccgcc aacgactgac gcaaattatc tgtgggcagc aggtcaattt tgttgatgtc
    13441 ctgaccagca acaataccaa tctgcaaatc agcttcgtcc attcgcgcta tcgtaatgaa
    13501 aacgtggcca tttgtgtgct ggtggatgtt tcttcgcgcg tgaagatgga agagtcgttg
    13561 caggagatgg cacaagcagc ggaacaggcg agccagtcaa aatcgatgtt ccttgccacc
    13621 gtcagtcatg agctgcgaac gccgctgtat ggcattatcg gtaacctgga tctgttgcaa
    13681 accaaagagt taccgaaagg cgtcgatcgg ctggtgacgg caatgaacaa ctcttccagc
    13741 ctgttgttga aaattatcag cgatattctc gatttctcga agattgaatc ggaacagttg
    13801 aagatcgaac cgcgtgagtt ttcaccgcgt gaagtgatga accacatcac cgccaactat
    13861 ttaccgctgg tggtacgcaa gcagttaggc ttgtactgct ttattgaacc ggatgtgcca
    13921 gtggccttaa atggcgaccc gatgcgttta cagcaggtca tctccaacct gttgagtaac
    13981 gccataaaat tcaccgatac cggctgtata gttttgcatg tccgcgctga tggcgattat
    14041 ctctctatcc gtgttcgcga taccggcgtg gggattccgg cgaaagaagt ggtgcgcttg
    14101 tttgatccct tcttccaggt cggaacgggc gtacagcgaa acttccaggg gaccggtctg
    14161 ggtctggcga tttgtgaaaa actgatcagc atgatggacg gcgatatctc ggtagattca
    14221 gaaccgggaa tgggcagcca gtttaccgtg cgtattccgt tgtacggcgc tcagtacccg
    14281 cagaaaaaag gcgtggaagg gttgagtggt aaacgctgct ggctggcggt ccgcaatgcg
    14341 tcgctctgtc agttcctgga aaccagtttg cagcgcagcg gcatcgtcgt tacaacatac
    14401 gaagggcagg aaccgactcc cgaagatgtg ttaattactg acgaggtagt gagtaaaaaa
    14461 tggcagggta gagcggtagt gaccttctgt cgtcgccata ttggtattcc gctggagaaa
    14521 gcgccagggg agtgggtaca cagtgtggct gccccgcatg agctaccggc attgttggcg
    14581 cgtatttatt tgatcgagat ggagagcgac gatcctgcta acgctctgcc gtcgacggac
    14641 aaagcggtca gcgataatga cgatatgatg attctggtcg tggatgatca tccgattaac
    14701 cgacgtctgc tggcagatca gttgggatcg ttgggctatc aatgtaaaac cgcgaatgat
    14761 ggtgtcgatg cgcttaatgt acttagcaag aatcatattg atatcgtgct tagcgacgtc
    14821 aacatgccaa atatggatgg ttaccgcttg acgcaacgca ttcgtcagtt gggactgacg
    14881 ttgccggtaa tcggagtaac tgctaatgcg ttggctgaag agaagcagcg gtgtctggag
    14941 tccggtatgg acagctgcct gtcgaagccg gtaacgctgg atgtgataaa acagacgctg
    15001 acggtatatg ccgagagggt caggaaatcg cgggaatcgt aggacggata aggtgtttat
    15061 gccgcatctg gcattcagtg caaatgccag atgcgacgct gacgcgtctt atctggccta
    15121 ctttaatgta atctgttgaa ataatgggaa tcgtaggccg gataaggcgt ttacgctgca
    15181 tccggcaatc ggtgcaaatg ccagataaga cactaacgcg tcttatctgg cctacagatg
    15241 attagtcttt atctgccgga cttaaggtca cagaagagag ataattcagc agggcgatat
    15301 cgttctcgac acccagcttc atcatcgcag atttcttctg gctactgatg gttttaatac
    15361 tgcggttcag ctttttagcg atctcggtca ccaggaagcc ttccgcaaac aggcgcagaa
    15421 cttcactctc ttttggcgag agacgcttgt caccgtaacc accagcactg attttttcca
    15481 acaggcgaga aacgctttcc ggggtaaatt tcttcccttt ctgcagcgcg gcgagagctt
    15541 tcggcagatc ggtcggtgca ccttgtttca gcacgatccc ttcgatatcc agatccaata
    15601 ccgcactaag aatcgccggg ttgttgttca tggtcagaac aatgatcgac aggcttggga
    15661 aatggcgctt gatgtacttg attaaggtaa tgccatcgcc gtacttatcg ccaggcatgg
    15721 agagatcggt aatcaacaca tgcgcatcca gtttcggcag gttgttgatc agtgctgtag
    15781 agtcttcaaa ttcgccgaca acattcaccc actcaatttg ctcaagtgat ttgcgaatac
    15841 cgaacaagac tatcggatgg tcatcggcaa taattacgtt catattgttc atgtaatagg
    15901 ctaccttgct acagcaagct cttgacataa ctgtcaatgt cgctgatgta tttttctatt
    15961 cctggaacat ccttctcacg aatcagatgt tccagcgttt cacataactg cttgccgggt
    16021 accagattta gcatggcaaa tacgccttta agacgatgag ccgtttgggc taacgcagca
    16081 aagtcactgg ttgctgcttc agtatacagc ctcttaacat catccggtac tgtgtctaca
    16141 aagagcgcat aatagccgct ggcatggagt tgcgcatttt catctccgcc cagaggcgat
    16201 tctgtcacct cttcctgcgc cagttgcact tcaattaatt gtaagaccgc ttcctgcata
    16261 gcgttgctca tattgaagtt gacgcacaat tgaccaggcc caatttcccg tacgccagac
    16321 tcatcatcgc ttaaaagcaa gccagaggca gtaagattag acggattatc cgttaaaaag
    16381 atatcataat cttgactaat taatctttca tcgggtgtga tacaggttgc accccaattt
    16441 tctaactggc gagtgacaat attccgaatt tctgccgaag taacatccac cattacgcag
    16501 acatcatcca gtaaacgctc ttcttcctct tcaacttccg ggtcagctgc gagcattttg
    16561 atatgcacag agtagcgtgt accaagccca tcccgcgttt tgatgtttaa atgaccgccc
    16621 agtttacgtg ccagttgatc gctcagccag aatgccagcg ggtccgcctt gccatagcga
    16681 tcgttttggg tctggttgat aaacgggaag tgcaaattat ccatttcatg aatacttacg
    16741 ccttctcccg tgtccagaat gcggaacgtc aggcggtctt cggaggactc atcctgatca
    16801 acctcaaggg tgatttttcc caattgcgtt gaggtcacgg catattgcat cagtagcagc
    16861 aaaatacgtc gtaaggcatc gcgatcgccg cggcgcatat cgtgtgcttt cagatgattg
    16921 ttaatcagca gttgcagacc tttacgcttg atggcaggca acactgaagg cacaacttca
    16981 tcaattaaat cctgcacgga gaacagcacc gtctcacttt tccagctatc atccgcaagc
    17041 atgttcgcta actgtatttc atcaaccagc cgcaccagca catctgcctg attcgccagt
    17101 tgtttgcttt ccggggcgtt gagtttagcc gcgctctccg ccagggactg tgcgggttct
    17161 ttcagcgcat cgccaatgtt tttcataaag gtcatccgcc cctgctggtt tttctcatac
    17221 agacgctgcg cctgcttgag tttcttgttt accagcactt cgcgatcctg atcgcgaata
    17281 atgaaaattt gtgtgcgcgg cgcgacctgg ctgcggaaca tgcggatctc atacagctcg
    17341 ttattgatcg tcgcctgaat aatcccctga tgctgttccg ccatggtggt gatgttttgc
    17401 agattcaaat gcggcagcaa atgatcggca attttgttac ttatgacagt gcggttcgat
    17461 tcctgatcgt gaaccagcag gccgagcggc agcagtgaga ctatctcttc attaattgcc
    17521 cgtaaaatgc gcaattcgtt attgaccgcc gtgctgggta gactttctgt actgcggctg
    17581 gagaaatggc ggaatgtggt atagccaaat aacgccagcg ccagcaaacc gatgttcagc
    17641 agcagtggca gcagaatgtt ttgcaacgta tccagcaata aggtgccata aggaacctgc
    17701 cagaccaggc gcatatcggt agagttgagt gccgaggaga tttcaatctt cgtactgtta
    17761 aagtggatac tgacgctatc cgtcccttct ttctcattat cattgtttcc cgttgccgtc
    17821 gcgtctggct caaggcggaa actgtccagc ggcatacccg gtgggatcaa atcattaatc
    17881 ggcagatcaa aagccacgac cgttgccaga tgtcctggct ggttaaaggt agtacgcaag
    17941 gtaaagtaat gaccgttctg ccaggccagg cggcgcatgt tagaaaagct ttcgcgttca
    18001 tcgagggcgt tggcctgttg caacatctct gcacgacgtg aatcaacaat gtcgctgacg
    18061 gtcgattctt taaatccgga ggtgagatct ttaaggggca gcgttgagat cagcaccaga
    18121 ctgttatcct gaccattcag gtaatacatc gaccacggta cattttctgc gccccacaat
    18181 gtgtccagat aggtggacat ccgctgagtc atctcaagcg ttgagttgtc gtgagagcca
    18241 aagattaacg cttccgtttt gcggcgcggt ttctccagat agtagacatc ctgtttcagg
    18301 cgcgtctctt gtaaaccttc gccggaggag ggggaggtcg tcgcggcaat gttgtcgtag
    18361 atctgccagg tcacgtaacg ccaggtatcg acgcgctttt ggatagcgtg ggtaatgtcg
    18421 acaatctggt aacttttatc tttcagccat gcgttaacgg cgctttgtac cattacaccc
    18481 atcgtcacca gtaacacaat gatcaacagt aaaaagaagc gggtaatgct ccccggtagg
    18541 agtgaaaagc gggtcgtggc cgttgtctct ttctgacgca ttcgtgttta tgacctgtta
    18601 aaacttcgcg aagtgatagg gcagtataaa gggtacagtg tgaaattcca gactcttacc
    18661 tgattgatga ttctctattc tcccgtgata tttatcatcc tgcattgggg gaaatagatg
    18721 tacaagcgcc attttgctgt acaaataacc tacaaaaagc ccaacgaaaa taattgcaaa
    18781 taaacaaaga ttgctggaaa ttatgctgat gttaattatt tgtgaaatag ttaacaagcg
    18841 ttatagtttt tctgtggtag caaagaataa tgaaaagtgt gtaaagaagg gtaaaaaaaa
    18901 ccgaatgcga ggcatccggt tgaaataggg gtaaacagac attcagaaat gaatgacggt
    18961 aataaataaa gttaatgatg atagcgggag ttattctagt tgcgagtgaa ggttttgttt
    19021 tgacattcag tgctgtcaaa tacttaagaa taagttattg attttaacct tgaattatta
    19081 ttgcttgatg ttaggtgctt atttcgccat tccgcaataa tcttaaaaag ttcccttgca
    19141 tttacatttt gaaacatcta tagcgataaa tgaaacatct taaaagtttt agtatcatat
    19201 tcgtgttgga ttattctgca tttttgggga gaatggactt gccgactgat taatgagggt
    19261 taatcagtat gcagtggcat aaaaaagcaa ataaaggcat ataacagagg gttaataaca
    19321 tgaaagttaa agtactgtcc ctcctggtcc cagctctgct ggtagcaggc gcagcaaacg
    19381 ctgctgaagt ttacaacaaa gacggcaaca aattagatct gtacggtaaa gtagacggcc
    19441 tgcactattt ctctgacgac aagtctgtag atggcgacca gacctacatg cgtcttggct
    19501 tcaaaggtga aactcaggtt actgaccagc tgaccggtta cggccagtgg gaatatcaga
    19561 ttcagggcaa cagcgctgaa aacgaaaaca actcctggac ccgtgtggca ttcgcaggtc
    19621 tgaaattcca ggatgtaggt tctttcgact acggtcgtaa ctacggcgtt gtttacgacg
    19681 taacttcctg gaccgacgta ctgccagaat tcggtggtga cacctacggt tctgacaact
    19741 tcatgcagca gcgtggtaac ggctttgcga cctaccgtaa cactgacttc ttcggtctgg
    19801 ttgacggcct gaactttgct gttcagtacc agggcaaaaa cggtagcgct agaggcgaag
    19861 gcatgaccaa caatggtcgc gatgctctgc gtcagaacgg cgacggcgtt ggcggttcta
    19921 tcacttatga ttacgaaggc ttcggtatcg gtgctgcagt ttccagctcc aaacgtactg
    19981 atgctcagaa caccgctgct tacatcggta acggcgaccg tgctgaaacc tacactggtg
    20041 gtctgaaata cgatgctaac aacatctacc tggctgctca gtacacccag acctacaacg
    20101 caactcgcgt aggttccctg ggttgggcga acaaagcaca gaacttcgaa gctgttgctc
    20161 agtaccagtt cgacttcggt ctgcgcccgt ctgtagcata cctgcagtct aaaggtaaaa
    20221 acctgggcac catcggtact cgtaactacg acgacgaaga tatcctgaaa tatgttgatg
    20281 ttggtgcgac ctactacttc aacaaaaaca tgtccaccta cgttgactac aaaatcaacc
    20341 tgctggacga caaccagttc actcgtgacg ctggcatcaa cactgataac atcgtagctc
    20401 tgggcctggt ttaccagttc taatctcgat tgatctcgaa caaagggcct gcgggccctt
    20461 ttttcattgt tttcagcgta caaacacagt tttttggtgt actcttgcga ccgttcgcat
    20521 gaggataatc acgtatggaa ataagcttta cccgcgtggc actgctggct gccgcgctct
    20581 tctttgttgg ttgcgatcaa aaaccacaac ccgccaaaac ccacgctact gaagttaccg
    20641 ttcttgaagg caaaaccatg ggtaccttct ggcgtgccag catcccgggc attgacgcca
    20701 aacgcagtgc cgaacttaaa gaaaagattc agacccagct ggatgccgac gatcagctgc
    20761 tttcgaccta taaaaaagat tccgcgctga tgcgctttaa cgactcgcaa agtttgtcgc
    20821 cgtggccggt aagtgaagcg atggccgata tcgtcaccac ctcgctgcgc attggcgcaa
    20881 ggaccgatgg tgcgatggat ataaccgtcg ggccgctggt gaatctgtgg ggctttggcc
    20941 cggaacaaca gccggttcaa attccgagcc aggaacagat cgatgcgatg aaagccaaaa
    21001 ccggcttaca gcacctgacg gtcatcaatc agtcgcatca gcaatatctg caaaaagacc
    21061 tgccggattt atatgtcgat ctctccaccg tcggtgaagg ttatgcggcg gatcacctgg
    21121 cacgcttgat ggagcaggaa gggatttccc gctatctggt gtcggtgggc ggcgcgctga
    21181 acagccgtgg tatgaacggt gaaggccagc cgtggcgggt agcgattcaa aaaccaaccg
    21241 ataaagaaaa cgcggttcag gctgtggtgg atatcaacgg tcatgggatt agcacctctg
    21301 gcagctaccg taactattac gaactggacg gcaaacgtct ttcccatgtt atcgatccgc
    21361 aaaccgggcg tcccatcgaa cacaatctgg tatccgtgac ggtgattgct ccgacggcgc
    21421 tggaagccga tgcctgggat actggcttga tggtactcgg gccgaagaaa gccaaagagg
    21481 ttgttcgccg ggaagggctg gcggtttata tgatcaccaa agaaggcgat agctttaaaa
    21541 cctggatgtc accacagttt aaaagcttcc ttgtcagcga aaaaaattaa agcgcaagat
    21601 tgttggtttt tgcgtgatgg tgaccgggca gactaaaggc tatccttaac cagggagctg
    21661 attatgaaaa aagccacatg cttaactgac gatcaacgct ggcaatctgt cttagcccgc
    21721 gacccgaatg ccgacggcga attcgttttc gccgtgcgta ccacaggcat cttttgccgt
    21781 ccgtcttgcc gcgccagaca tgctttgcgg gaaaacgtct ccttctacgc aaatgccagc
    21841 gaggcactcg ccgctggctt tcacccctgc aaacgttgtc agccagaaaa agccaatgcc
    21901 cagcaacatc ggttggataa aatcacccac gcgtgtcgac tgctggaaca ggaaacgcct
    21961 gtaacgctgg aagccttagc cgaccaggtg gcgatgagtc catttcatct acatcggttg
    22021 tttaaagcga ctaccggaat gacgcctaaa gcctggcaac aggcctggcg cgctcgccgt
    22081 ttgcgcgaat cgctggcgaa aggggagagc gtgacgacgt ctattcttaa cgccggattc
    22141 cccgacagca gcagttacta tcgcaaagct gacgaaacgc tgggcatgac ggctaaacaa
    22201 ttccgtcacg gtggcgaaaa tctggcggtg cgttacgcgc tggctgattg tgagctgggt
    22261 cgttgcctgg tggcagaaag cgagcggggg atttgcgcga tattgctggg cgatgatgac
    22321 gcgacactaa tcagcgagtt gcagcagatg tttcccgctg ccgacaacgc gcctgccgat
    22381 ctgatgtttc agcaacatgt gcgtgaagtg atcgccagcc tcaatcaacg cgatacgccg
    22441 ctgacgttac cgctggacat tcgcggcact gcttttcagc aacaagtctg gcaggcactg
    22501 cgcacgatac cttgcggtga aaccgtcagt tatcagcaac tggctaacgc catcggcaaa
    22561 ccgaaagcgg tacgggccgt tgccagcgcc tgtgccgcca acaagctggc tatcataata
    22621 ccctgtcatc gggtggtccg tggtgatggc acactttccg gttaccgctg gggcgtgtcg
    22681 cgtaaagcgc aactgctgcg ccgcgaagct gaaaatgagg agaggtaatg ttggatctgt
    22741 ttgccgatgc tgaaccgtgg caagagccac tggcggctgg agcggtaatt ttgcaccgtt
    22801 ttgcttttaa cgctgcggag caactgatcc gcgatattaa taacgttgcc agccagtcgc
    22861 cgtttcgcca gatggtcacc cccgggggat ataccatgtc ggtggcgatg accaactgtg
    22921 ggcatctggg ctggacgagc catcggcaag gttatctcta ttcgcccatt gatccgcaaa
    22981 caaataaacc gtggcccgcc atgccacaga gttttcataa tttatgtcaa cgtgcggcta
    23041 cggcggcggg ctatccagat ttccagccag atgcttgtct tatcaaccgc tacgctcctg
    23101 gcgcgaaact gtcgctgcat caggataaag acgaaccgga tctgcgcgcg ccaattgttt
    23161 ctgtttctct gggcttaccc gcgatttttc aatttggtgg cctgaaacgt aatgatccgc
    23221 tcaaacgttt gttgttggaa catggcgatg tggtggtatg gggcggtgaa tcgcggctgt
    23281 tttatcacgg tattcaaccg ttgaaagcgg ggtttcatcc actcaccacc gactgccgct
    23341 acaacctgac attccgtcag gcaggtaaaa aagaataaaa ataagaatta ttattgctgt
    23401 gcgcgcgaag atgtttaaac tgcgggctgt aattcattgt ccgggttttc tgcatggaac
    23461 ttcttgtact tgtctggcgg cagtatcgct ggccatttat cagtgtgatg gcgctaagcc
    23521 tcgccagtgc ggcattaggc attggcttaa ttgcttttat caatcagcgc cttatcgaaa
    23581 cggcggatac cagtctgctg gtgttgccgg agtttcttgg attattactg ctgttgatgg
    23641 cagtcactct cggatcgcaa ctggcgctca ccactttggg gcatcacttc gtttaccgac
    23701 tgcgtagtga atttatcaag cggattctgg atacccacgt cgagcgcatt gaacaactcg
    23761 gtagcgcctc gttgctggcg ggattaacca gcgatgtgcg caatatcacc attgcttttg
    23821 tgcgtcttcc ggaactggtg caggggatca ttctcactat cggttccgcg gcgtatctgt
    23881 ggatgctgtc gggcaaaatg ttgctggtaa cggctatctg gatggcgatc accatctggg
    23941 gtggttttgt actggtggcg cgggtgtaca aacatatggc gaccctgcgt gaaaccgaag
    24001 acaagctgta cacggatttt caaaccgtac tcgaagggcg caaagagctg actctgaacc
    24061 gggaacgcgc cgagtatgtg tttaacaacc tctacatccc cgatgcgcaa gagtatcgcc
    24121 atcatattat ccgcgcagac accttccatc ttagcgccgt gaactggtca aacatcatga
    24181 tgctgggcgc tatcggcctg gtgttctgga tggcgaacag tctcggttgg gctgatacca
    24241 acgttgctgc gacctattcg ttgacgctgt tattcctgcg tacgccgctg ctttcggcgg
    24301 ttggcgcatt gccgacgctg ctgacggcgc aggtggcgtt taacaagctg aacaaattcg
    24361 cgctcgcgcc tttcaaagct gagtttccgc gcccgcaggc ttttcccaac tggcaaacgc
    24421 tggagctgcg taacgtgacg tttagctatc aggataacgc gttttccgtt gggccgatta
    24481 acctcaccat taaacgtggc gagctgctgt ttctgattgg tggcaacggt agtggaaaat
    24541 cgacgctggc gatgttgctg acgggcttgt accagccaca aagcggcgaa atcttgctgg
    24601 atggcaaacc tgtcagtggc gaacaaccgg aagattatcg caaactgttt tcggcagtgt
    24661 ttaccgatgt ctggctgttt gatcaactgc tggggccgga gggtaaaccc gctaacccgc
    24721 aactggttga gaagtggctg gcgcagctga aaatggctca taagcttgag ttaagcaacg
    24781 ggcgtattgt taacctgaag ttatcaaaag ggcagaaaaa acgcgtggcg ctgttgctgg
    24841 cgctggcaga agaacgcgat attatcctgc tggatgaatg ggcggcggat caggatccac
    24901 acttccgtcg tgagttttat caggtgttgc tgccgctgat gcaggagatg ggtaaaacta
    24961 ttttcgctat cagtcatgat gatcattact ttatccacgc cgaccgcctg ctggaaatgc
    25021 gcaatgggca acttagcgag ctgacgggcg aagagcgcga tgccgcttcg cgtgatgccg
    25081 ttgcccggac ggcataatat tacgacgctg acctggcgga ctgctgccgt caggtcaata
    25141 tgctaaaaat cccttcattt ctaataaccc tataattaat caacgaaatt ataatgtttc
    25201 taaaattaga atataattta taaacattat ttaaatgttg ttgcttaagt gttaccgttg
    25261 atgccgcgca aatctcacct gcaataaagc gactaaaagt aaggcattaa caagatgaaa
    25321 aaagtgactg ccatgctctt ctcgatggcc gtggggctta atgccgtttc gatggcggca
    25381 aaagcgaaag cgtccgagga gcaggaaact gatgtactgt tgattggcgg cggcattatg
    25441 agcgccacgt tggggaccta tttacgcgag ctggagcctg aatggtcgat gaccatggtg
    25501 gagcgcctgg agggtgtcgc gcaggagagt tcgaacggct ggaataacgc cggaaccggg
    25561 cattctgcac tgatggaact gaactacacc ccgcaaaacg ccgatggcag catcagtatt
    25621 gaaaaagcag tcgccattaa cgaagcattt cagatttccc gccagttctg ggcgcaccag
    25681 gttgagcgcg gcgtgctgcg tactccgcgt tcatttatca ataccgttcc gcatatgagc
    25741 tttgtctggg gcgaggataa cgttaatttc ctgcgcgccc gttacgccgc gttgcaacaa
    25801 agctcgctgt ttcgcggtat gcgttactct gaagatcacg cgcagatcaa agagtgggca
    25861 ccgttagtga tggaagggcg cgatccacaa cagaaagtag cggcaacacg cacagaaatt
    25921 ggcactgatg tgaactatgg cgagatcacc cgccagttaa ttgcttcctt acagaagaaa
    25981 tctaacttct cgctgcaact cagcagcgca gtccgcgccc taaagcgtaa tgacgataac
    26041 acctggaccg ttaccgttgc cgatctgaaa aacggcactg cacagaacat ccgcgccaaa
    26101 tttgtcttta tcggcgcggg cggtgcggcg ctgaagctat tacaggaatc ggggattccg
    26161 gaagcgaaag actacgccgg tttcccggtg ggcggacagt tccttgtttc ggaaaacccg
    26221 gacgtggtta atcaccatct ggcgaaggtt tacggtaaag catccgttgg cgcaccaccg
    26281 atgtcggttc cgcatatcga tacccgcgtt ctggacggta aacgcgtagt gctgtttggg
    26341 ccatttgcca ccttctcaac caaattcctc aaaaacggtt cattgtggga tctgatgagt
    26401 tccaccacca cctctaacgt gatgccgatg atgcacgtcg ggctggataa tttcgatctg
    26461 gtgaaatatc tggtgagtca ggtgatgttg agtgaagagg atcgttttga agcgttgaaa
    26521 gagtactatc cgcaggcaaa aaaagaggac tggcgtttgt ggcaagcggg gcagcgcgtg
    26581 cagattatca agcgtgatgc cgagaaaggt ggcgtactgc gtctgggtac tgaagtggtc
    26641 agtgaccagc aagggaccat tgccgcgctg ctgggggcat cgccaggggc gtcaaccgcc
    26701 gcgccgatta tgttggatct gctggaaaaa gtatttggcg atcgcgtttc cagcccgcaa
    26761 tggcaggcta cgttgaaagc gatcgttccg tcttatggac gcaagctgaa cggtgatgta
    26821 gcggcaacag aacgcgagtt gcagtacacc agcgaagtgc tggggctgaa atacgacaag
    26881 ccgcaggcag cagatagtac gccaaaaccg cagttgaaac cgcaacccgt tcaaaaagaa
    26941 gtggcggata ttgcgttgta atgatgtgcc acatccggca tggtatgccg gatgtggcgt
    27001 atgctgataa gacgcgccag cgtcgcatca ggcaccggct cgggcgttag atgtcggatg
    27061 cggcgtgacc gccttattcg gcctacgggg tgccgcactt cacagcattt tagcgaacta
    27121 ccgcgttgtc aattttctct tccgccttcc agacgcggta cttcacatct acattgtctg
    27181 gcgtatacac cacgatcggc agcttgctgt tgtaacgcag cattccagca tcgcccagat
    27241 acgcggtgac aaatttcttc tctttcttgc catccgggca ggccatcatc gtcgaaaccg
    27301 gggaactgac tttatcaaag acataataat catagcccca gccttccagc gttttgtttt
    27361 ccagcttccc gccgagacga tgcaaattgc aatcgacttc cagcgtctga ccgattaaca
    27421 gttctacttt cagggtagat tcatcttctt gcggggttaa ctgaatcacc tgacgcttca
    27481 tccctttttc agcttgtgga taaggcgcga ttttttccag tggctggacg ctttctgccg
    27541 cccaggcgga agtggtagcg aaagcggcaa acaatactgc aggtagaatg gtcttcattt
    27601 ttttgctttc cttcttcatt tataggtttt caccgatgat gtcgctgaag gttaacatat
    27661 tcatcagatt tattaatttg ctttatgaat aacttaaaga acattcagac aattttacct
    27721 gctcttt
//
LOCUS       DAZGVQ010000059        27333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000059 DAZGVQ010000000
VERSION     DAZGVQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.059"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(40..429)
                     /gene="mutT"
                     /locus_tag="ACHLAG_002379"
     CDS             complement(40..429)
                     /gene="mutT"
                     /locus_tag="ACHLAG_002379"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFP0655594.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(489..3194)
                     /gene="secA"
                     /locus_tag="ACHLAG_002380"
     CDS             complement(489..3194)
                     /gene="secA"
                     /locus_tag="ACHLAG_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFP0655595.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(3256..3768)
                     /gene="secM"
                     /locus_tag="ACHLAG_002381"
     CDS             complement(3256..3768)
                     /gene="secM"
                     /locus_tag="ACHLAG_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFP0655596.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(3999..4916)
                     /gene="lpxC"
                     /locus_tag="ACHLAG_002382"
     CDS             complement(3999..4916)
                     /gene="lpxC"
                     /locus_tag="ACHLAG_002382"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFP0655597.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(5017..6168)
                     /gene="ftsZ"
                     /locus_tag="ACHLAG_002383"
     CDS             complement(5017..6168)
                     /gene="ftsZ"
                     /locus_tag="ACHLAG_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP0655598.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(6229..7491)
                     /gene="ftsA"
                     /locus_tag="ACHLAG_002384"
     CDS             complement(6229..7491)
                     /gene="ftsA"
                     /locus_tag="ACHLAG_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFP0655599.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(7488..8318)
                     /gene="ftsQ"
                     /locus_tag="ACHLAG_002385"
     CDS             complement(7488..8318)
                     /gene="ftsQ"
                     /locus_tag="ACHLAG_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFP0655600.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(8320..9240)
                     /gene="ddlB"
                     /locus_tag="ACHLAG_002386"
     CDS             complement(8320..9240)
                     /gene="ddlB"
                     /locus_tag="ACHLAG_002386"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP0655601.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQELVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(9233..10708)
                     /gene="murC"
                     /locus_tag="ACHLAG_002387"
     CDS             complement(9233..10708)
                     /gene="murC"
                     /locus_tag="ACHLAG_002387"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFP0655602.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(10762..11829)
                     /gene="murG"
                     /locus_tag="ACHLAG_002388"
     CDS             complement(10762..11829)
                     /gene="murG"
                     /locus_tag="ACHLAG_002388"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFP0655603.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPLLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(11826..13070)
                     /gene="ftsW"
                     /locus_tag="ACHLAG_002389"
     CDS             complement(11826..13070)
                     /gene="ftsW"
                     /locus_tag="ACHLAG_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFP0655604.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(13070..14386)
                     /gene="murD"
                     /locus_tag="ACHLAG_002390"
     CDS             complement(13070..14386)
                     /gene="murD"
                     /locus_tag="ACHLAG_002390"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFP0655605.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(14389..15471)
                     /gene="mraY"
                     /locus_tag="ACHLAG_002391"
     CDS             complement(14389..15471)
                     /gene="mraY"
                     /locus_tag="ACHLAG_002391"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFP0655606.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(15465..16823)
                     /gene="murF"
                     /locus_tag="ACHLAG_002392"
     CDS             complement(15465..16823)
                     /gene="murF"
                     /locus_tag="ACHLAG_002392"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFP0655607.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQLITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(16820..18307)
                     /gene="murE"
                     /locus_tag="ACHLAG_002393"
     CDS             complement(16820..18307)
                     /gene="murE"
                     /locus_tag="ACHLAG_002393"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFP0655608.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(18294..20060)
                     /gene="ftsI"
                     /locus_tag="ACHLAG_002394"
     CDS             complement(18294..20060)
                     /gene="ftsI"
                     /locus_tag="ACHLAG_002394"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFP0655609.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(20076..20441)
                     /gene="ftsL"
                     /locus_tag="ACHLAG_002395"
     CDS             complement(20076..20441)
                     /gene="ftsL"
                     /locus_tag="ACHLAG_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFP0655610.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(20438..21379)
                     /gene="rsmH"
                     /locus_tag="ACHLAG_002396"
     CDS             complement(20438..21379)
                     /gene="rsmH"
                     /locus_tag="ACHLAG_002396"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFP0655611.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(21381..21839)
                     /gene="mraZ"
                     /locus_tag="ACHLAG_002397"
     CDS             complement(21381..21839)
                     /gene="mraZ"
                     /locus_tag="ACHLAG_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFP0655612.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(22441..23445)
                     /gene="cra"
                     /locus_tag="ACHLAG_002398"
     CDS             complement(22441..23445)
                     /gene="cra"
                     /locus_tag="ACHLAG_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFP0655613.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(23527..23613)
                     /locus_tag="ACHLAG_002399"
     CDS             complement(23527..23613)
                     /locus_tag="ACHLAG_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFP0655614.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(23625..24116)
                     /gene="ilvN"
                     /locus_tag="ACHLAG_002400"
     CDS             complement(23625..24116)
                     /gene="ilvN"
                     /locus_tag="ACHLAG_002400"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP0655615.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(24119..25843)
                     /gene="ilvI"
                     /locus_tag="ACHLAG_002401"
     CDS             complement(24119..25843)
                     /gene="ilvI"
                     /locus_tag="ACHLAG_002401"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFP0655616.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKGALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            25962..26006
                     /locus_tag="ACHLAG_002402"
     CDS             25962..26006
                     /locus_tag="ACHLAG_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655617.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(26161..27105)
                     /gene="leuO"
                     /locus_tag="ACHLAG_002403"
     CDS             complement(26161..27105)
                     /gene="leuO"
                     /locus_tag="ACHLAG_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFP0655618.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         6545 a         7768 c         6554 g         6466 t
ORIGIN      
        1 tgtgcaaatg tcggatgcgg cgtgaacgcc ttatccgacc tacagacgct taagcttcgc
       61 aattaccggt tcattggctg gcggaaaatc atcggcatta agaccgacta gcgacatcca
      121 ctcaccgggt tgcccttctt taccccacgg ctccccttcc cagctttcga ccagccagaa
      181 ccacagagtt atatgcctgt ccgggaattc atattccagt ttttcaaata gcgaaaaatg
      241 ttgtggggta atcccgactt cttcctgaag ttcacgcacc accgcctgtt ccggcgtttc
      301 gcccatttca attttaccgc cgggaaactc cagtttattc gccatgtgcg catctgctgc
      361 gcgacgcgtt ataaagattt cattgttctc gttgcgaata atacctaccg caatttgcag
      421 ctttttcatt gtcttaaacc tataaaaaag gcgcagaatc ctgcgccttt tacttcaact
      481 gttagctttt attgcaggcg accatggcac tgcttgtatt ttttaccaga accgcacggg
      541 caaggatcgt tacgtcctac tttgcgctca ccggtttgcg ccgccagtgc agctgcggct
      601 gcagagtcgt catcctgatg gctaagctgc tgcatttgcg ctaaacgctc ggcttccata
      661 cgacgctgtt gttccagctc ctcaacctct tcaggcatac gtacctggac tttgctcagc
      721 gtgctgataa cttcatattt taacgactcc agcatcgctg caaacatgga gaacgattca
      781 cgtttgtatt cctgcttcgg atctttctgt gcatagccac gcaggtggat accctgacgc
      841 agatagtcca tcgccgccag gtgctctttc cacagagagt cgagagtttg cagcatgacg
      901 cctttttcga agtgacgcat catctcagca ccaaccactt cttctttacg ctgatacact
      961 tcgatggact gcgccagaat tcgctcacgc agcgtctctt catgcagttc tggttcttta
     1021 tccagccact cggcaattgg caaatcgagg tcgaaatcgt tcttcagacg ttcctgcagc
     1081 cccggaatat cccacatttc ttccagtgac tgtggcggaa tgtaggcatc aatggtcgct
     1141 ttgaacacat cttcacgaat gctgttgatg gtttcgctca catcgctgac atccagcagt
     1201 tcgttacgct gggagtaaat ggcgcgacgc tgatcgttag ccacgtcatc atattccagc
     1261 agttgcttac gaatgtcgaa gttacggctt tcaactttac gctgggcgtt ggcaatcgct
     1321 ttagtcaccc acgggtgttc aatggcttcg cctggcttca tacccagttt acgcatcatg
     1381 ccggatactc ggtcggaagc aaaaatacgc atcagcgcat cttccatcga caggtagaaa
     1441 cgggaagaac cagcatcccc ctgacgacca gaacgaccgc gcaactggtt atcgatacga
     1501 cgggattcgt gacgctcggt accgatgata tgcaggccac ctgcttccag taccgcatcg
     1561 tgacgtacct gccagtcggc tttaattttt tcaatttgct ctgcggtcgg attttccagc
     1621 gcggcaactt ctgcctgcca gctaccaccg agcacaatat ctgtaccacg acccgccata
     1681 ttggtcgcga tagtcaccgc agccggataa cctgcctgag caacaatcgc cgcttcgttg
     1741 gcgtggaatt tggcgttcag gacgttgtgc ttaataccgg ctttggtcag ttcgtttgac
     1801 accagctccg atttttcgat ggagatagta cccaccagca ccggctggcc tttcgcagta
     1861 cgttctttga tatcttcaat gatcgcctga attttttccg cttcagtcat gtagaccagg
     1921 tccggcagat ctttacgaat cattggacgg ttggtcggaa caacgacggt atccagcttg
     1981 tagatggagc taaattcgaa agcttcggta tcagcagtac cggtcatccc cgccagtttt
     2041 tcatacagac ggaagtagtt ctggaaggtg atcgaagcca gcgtttggtt ttcgttctgg
     2101 atctgcacac cttctttcgc ttccacagcc tggtgcagac catcggacca gcgacggccc
     2161 tgcatggtac gaccggtgtg ttcgtcaacg atgataactt caccatcttt aacgatgtag
     2221 tcgacgtcgc gggtaaacag cgcatgagcg cgcagcgccg ccgttacgtg gtgcatcagc
     2281 atgatgttgg ccggagagta cagagactcc ccttcatcca tgatgccctc tttcaccagc
     2341 agttcttcaa tcagcaccag accacgttcg gtcaggttca cctggcgaga tttttcgtcc
     2401 accgagaagt ggccttcgcc ctggaaggtt tcggagtctt ctttttcctg acggatcagg
     2461 tgcggaataa ttttattcac gcgtttatac atttccgagc tgtcttctgc cgggccggaa
     2521 atgatcagcg gtgtacgcgc ttcatcgatc aggatggagt ccacttcgtc caccagcgca
     2581 tagtgcagtt tacgctgtac acgttcttca gggctgaacg ccatattgtc gcgcaggtag
     2641 tcaaagccgt attcgttgtt cgtaccgtaa gtgatgtcag ctgcgtaagc ttcgcgcttt
     2701 gccggtgctg gcatgcccgg caggttgata ccgacagtca ggccaaggaa ttcaaacagc
     2761 ggacggttgt tttcggcgtc acgttgcgcc aggtagtcgt tgacggtaac tacgtgcacg
     2821 cctttaccgg ttagtgcgtt caggtaagca ggcagcgttg cggtcagggt tttaccttca
     2881 ccggtacgca tttcggcgat gcagcgttcg ttaagaacca taccgccgag taactgaacg
     2941 tcgaagtgac gcataccaaa gacgcgctta cttgcctcac gtaccacggc gaaagcttcc
     3001 gggatcagat tttccagcac ttcgcctttt tccagacgtg cacgaaactc tgcggttttc
     3061 cctttcagtt cttcgtcgga gagtttttcc atctccggtt ccatggcatt gatgatgttg
     3121 accactttgc gcatccggcg cagggtgcga tcgttacgac taccgaaaac tttagttaac
     3181 aatttgatta gcataataaa atctcaaacg ccccgcgttg cggagttaat aaaatgaagt
     3241 aaaggtttat tgttgttagg tgaggcgttg agggccagca cggatgcctt gcgcctggct
     3301 tatccagacg ggcgtgctga attttgcttg tggggtaaaa tgcgcataat caatgcgata
     3361 acccttttca gacggcgtgc cttcctgggt cagcagcgcg ctgagcgtat ccagtaatgc
     3421 aagatgttgc gcctgaagag gcaaagattc ttcagcaacg ggcagtgttt gcggtgccat
     3481 tgcgaaagaa agatgacgga ttaccgtgcg aatggcatgt tgatgccagt aatcaacgga
     3541 atagttcgaa ttcgggcggc gtgtgttcgc ttccagcaag gccaattgac caaagttaac
     3601 tttggctgaa ggctcgtggt tgcgggttgt cgcttttgcg ggcgcgtttg gttcggcggc
     3661 gttgctgagc gcaggcaaac ctaaactcgc cgcaaccatc cctaataaga gatgcggcca
     3721 gaagtagcgt ttaccaaact gtcgccagcg cgtcagtatt ccactcacgt tattgccatc
     3781 ccgatcatat tcaaacgtat ttagcgcaca aatcttatca ttaaagcctg cagtccacag
     3841 cacgaatgaa gataagttgt gcggaaaagt gcttaagaaa acagcgttcg caccgttttt
     3901 tatagctctt ataaaattca aaaaaaaacg actggttcac ctggccggag agagtgccag
     3961 atttgccagt cgaattttat acgacagtat aaatgtcgtt atgccagtac agctgaaggc
     4021 gctttgaagg ccaacggcag ttctgcgtcg tcctggaagg tcacatattc ccaggcttcc
     4081 tgtttcgcca ggacagcctg cagcagtttg ttattcagtg catgaccgga tttataagcg
     4141 gtaaatgcac caataatatt gtgaccacac atgaacaagt caccgatcgc atcgagcatt
     4201 ttgtgacgca caaattcgtc ttcaaaacgc aggccgtctt cgttcagtac gcgataatcg
     4261 tcaacaacga tggcacaatc gaagctgccg cccaggcaca aaccacggga ctgcagatat
     4321 tcgatatcac gcatgaaacc gaacgtacgc gcacggctga tctggcgcat aaacgcatca
     4381 gcggagaagt tcatcgcata gcgctggttg ctggaatcaa tagccggatg gttaaaatcg
     4441 atggtgaaat ccagcgaaaa accattgtac ggcttaaatt cagcccactt atcgccatct
     4501 tcgacacgaa cagtctcttt gatgcgaaca aattttttgg cgcagttcaa ctcgtcgata
     4561 ccggcgtcaa gcagcaggta tacaaacgga gcggcgctgc cgtccatgat cgggatttcc
     4621 ggcgcgttaa cttcgataac aatgttatcg atgcccaagc ccgcgagagc agcattgagg
     4681 tgctctacgg ttgaaatccg tacatcatgc tcgttgacca gacacgtaca gagcatggta
     4741 tcacgcacag atttggcatc ggccgggaaa tctaccggtg gattcaagtc ggtgcgacga
     4801 tagatgaccc cggtgttggc cggcgcaggg cgtaacgtca gggtgacttt cttgccggta
     4861 tgtaaaccga cacccgtcgc ctgaacgata cgtttaagtg tcctttgttt gatcatcgta
     4921 ttatctcgcc aaattaccta tccaaccgaa gtgtactata cattcggcgg gccagtttag
     4981 cacaaagagc ctcgaaaccc aaattccagt caattcttaa tcagcttgct tacgcaggaa
     5041 tgctgggata tccagataat ccggctcttt cgcagtttgc ggcgcattgt cattcacgac
     5101 tttagcaacc ggcttctgct cctgggtcag cggagccatc ccatgctgct ggtagcgatc
     5161 catcactggc tgctgaacct gcttattggt caccagagtg atttcaggac gtttgtccat
     5221 gccgatacct gtcgcaacaa cggttacgcg cagctcgtca ttcatatccg ggtcaagaga
     5281 agtaccgata accacagtcg cgttgtcgga agcaaatgca cggatggtgt tacctaccgt
     5341 ttcgaactca tccagacgca ggtcgaagcc cgccgtgatg ttaaccagca cgccgcgcgc
     5401 gccagacagg tcgatatctt ccagcagcgg agaagagata gccatttcag cagcttcttc
     5461 cgcacggtct tcaccgctcg ccacgccaga acccatcatt gcgtagccca tctcagacat
     5521 tacggtgcgt acgtctgcaa agtccacgtt catcaaaccc ggacgagtaa tcagttcagc
     5581 gataccttgc acagcgcctt tcagtacatc gttcgctgcg ccaaacgcat ccagcaggga
     5641 gataccgcgg cccagaactt tcagcagttt gtcgttcggg atagtgatca gagagtccac
     5701 atgcttggac agttcagtga tcccctgctc cgcgaatgcc atacgcttct tgccttcaaa
     5761 gttgaaaggc ttagtgacga cagcaacggt caggataccc aaatcttttg ccacttcagc
     5821 gacgactggt gctgcacctg taccggtacc accacccata cccgcagcaa taaagaccat
     5881 gtctgcacct tccagcgccg cacgcaatgc atcgcgatcc tcatcagccg cattgcggcc
     5941 aacttctgga ttagcgccag cgcccagtcc tttggtgata ccgctaccga tttgaatcgt
     6001 ctgtccaacc gctgttttac gcagcgcttg tgcatcggta tttaccgcga agaattcaac
     6061 accttcaatg cgctcgcgca ccatgtgttc aacagcatta ccgccgccgc cgccgacgcc
     6121 gatgacttta atcaccgcgt cattggtaag ttccattggt tcaaacatag tttctctccg
     6181 atttgtgcct gtcgcctgag gccgtaatca tcgtcggcct cataaaaatt aaaactcttt
     6241 tcgcagccaa ctattgagtc gcttgatcca cgagccaact gatgctgtaa cacgtttttc
     6301 tacttcagct tcaccgttaa gatgtgactc tttcccatag tgaagcaatc ccaccgccgt
     6361 cgaataatac ggctcctgag cataatccgt taaaccggta atgttcagcg gcgcgccgat
     6421 acgcacttgc gtatgaaaca cgcgctgagc acaggctgca agaccttcga tctgcgctgc
     6481 gccaccggtt aatacaatgc ctgccgccag gtgatgttta accccttgtt ggcgaagctt
     6541 ttcctgcaac tgcaatatct cttcgttgac caggttgagc agctcggtat agcgcggctc
     6601 gatcacctct gccagggtct gacgttgcag actccgcggc ggacgaccac ctacgctcgg
     6661 cacttccacg ctctcatctt ttccaacgat ggaacccagc gcacaaccgt ggcgaacttt
     6721 aatcgcttcg gcgtcgcttg gcggcgtgcc aaaggcataa gcgatatcac tggtcacgac
     6781 attgccagca taaggaatta ccttagtgtg gcgcaatgcc ccaccggtat aaacggcgat
     6841 atccattgta ccaccaccga tatcgacgac gcagacaccc agttcacgtt catcttccgt
     6901 caataccgaa taacttgatg ccagtccggc aaatatcagt tggtcaactt tcagcccaca
     6961 acgttcaacc gctttgacga tgtttttcgc catatcgttg tgacatgtga tcaggtgcac
     7021 ttttgcctgc atccgcacgc ccgaaagtcc taccggattc ttgatccctt cctgatagtc
     7081 aatcgcatac tcttgcggga tcacatgcag cacacgatgc tcatcgcgca cacgcaccga
     7141 tttcgcggta tggacgacgt tttccacatc ttcttgcgtc acttcttctt cagaaatagg
     7201 caccatacca atttcattct ggcagctgat gtgcttacca gaaagcgcca gatataccga
     7261 agagatctga caatctgcca tcaattctgc ctggtcaatg gcgcgttgta cgcacttgac
     7321 cacggattcg aggtcgttca ccccgccttt atccatacca cgtgacgggc agctgcccac
     7381 gccaatgata ttgaccatac cgtcgggcag aacttcccct actaaagcgg caaccttcgc
     7441 ggtaccaatc tccagtccta ctaccagttt tctgtccgtc gccttgatca ttgttgttct
     7501 gcctgtgcct gattttgttg ctgagtagat tcctctggcg gcaagggcgc ccagcctact
     7561 gccgctccag agtcataacg caaatcaacg tagctaatcc gtttgccatc ggtttgcgcc
     7621 tgctgctgta aaaccggata aagttctaca aagcgagcca aacgtttcat cgtatcgccc
     7681 cggccaagat tgagcttaat atcgttattc agcgtcaact gccaggaacg ccgcgcggtc
     7741 atcgccgctt ccttcagagt aaatctgtcc tttgccagca tctgccccat ttcgcgatag
     7801 ccctgcaaca cttcattggc gctgccttcc gggccataca gcattggaag cacctgcttg
     7861 ctggtgcgat ctggcggcac gctgaaggtg tttccttccg cgtctaccat atgttgatca
     7921 ttccaccgcg caatcggcac atattcaacc agatgaatct tcaattcatc aggccactgc
     7981 tttctgacgc tcacctgctt aatccacggc aggcgttgtt ctatttgcgt ctggatgatg
     8041 ttgacatctt gggtcataaa ggtacccggc tcacccaatg ccaggatcga ctgccggata
     8101 tcgtcattac gcgtgtaatg gcgttcaccg gtcaacacca gctttgagag cggcaggcgt
     8161 tgcgcatctt ccatccagcc caacacgacc cagccgctca ccaacactgt cgttaaaacg
     8221 gtcagcagga aaaggatccc cgccagacgc gttccattat tgcggcgaga agaaacctct
     8281 tcttcgctgt ttcgcgtgtt cagagcagcc tgcgacatat tagtccgcca gttccagaat
     8341 tcgtactacc aactgcgaga agctcatacc tgcctgacgt gccgccatcg gcaccaggct
     8401 gtggctggtc atacccggtg aggtattggc ttccagcaga taaaactgtc catcgctgtc
     8461 cagcataacg tcaatacgtc cccatccttt gcaacctaac gtcgtccatg ctttcagcac
     8521 taattcctgc aaattggcct cttgtgacgc ttccagacct gcggggcaga aatactgtgt
     8581 ctcatcagag agatacttcg cctcataatc atagaaggtt ccggacggtt gaatacgtat
     8641 tgacggtaaa atttcttcac cgagtatcgc aaccgtgaac tccggcccac ttagccattt
     8701 ttcaatcaat acttcttcat cgtgctgaaa tgccaatctt aatgcatctt gtagagcatt
     8761 ttctgctact acttttgaca ttcccacact ggaaccttcg cggctcggct taacgataac
     8821 cggcaaaccc agagcagaaa tttctgctaa ctgcttatca ctcaggcctt tttcaaactc
     8881 tgcgcgggtt aacgcaaccc acggagcgac cggtaaaccg gcaccttgcc acagcagttt
     8941 gctgcgtagt ttatccattg aaagcgcaga tgccatcact ccactgccgg tataaggcaa
     9001 gcccatcagc tcgagcatgc cctgcagcgt accatcttca ccgccgcgac cgtgtagcgc
     9061 gataaacact ttctgaaagc ccatcgactt cagttgcgtc acgtcgactt ctttcgggtc
     9121 gacaggatac gcatcaatac cgccttcacg cagcccggct aacaccgctg cgccagaatt
     9181 cagagaaact tcccgctcag cggaagtccc gcccaacagg accgcgattt tatcagtcat
     9241 gttgttcttc ctccggagtt tgcggcttca gtttgatttc agctaaagaa cgggcaattt
     9301 ttccgatatt accagccccc tgaacgagaa tcaggtcgtt accggttaat accggtgcca
     9361 gcatctcggc tacctgcgca gggtccggca ccagaatggg atcaattttc ccacgtccac
     9421 gaattgtgcg acacagcgaa cggctgtccg ctcccggaat tggcgcttcg ccagccggat
     9481 acacttccag catcaacagg gtatcaacct gcgtcagcac attggcgaaa tcatcataca
     9541 ggtcgcgcgt acgggtaaaa cggtgcggct gaaacagcat taccaggttt ttatccggcc
     9601 agcctgcgcg cgccgcttta atggtggcgt caacttccgt cgggtggtgg ccgtagtcat
     9661 cgaccagcat tgccgtaccg cttttaccat tcactggctc cagcgggaat tcaccgagga
     9721 aatcaaaacg gcgaccagtc ccctggaagc tctccagcgc acgcaaaata gcctcgtcgt
     9781 caattccctc ttccgtagca accgcaaccg cagctgcggc gttcagcgcg ttatgacggc
     9841 ctggcgcatt cagggtgacg cgcatcggct ctttgtcctg gcgcagcagc gtaaagtgcc
     9901 cctgcgggcc aatctgctga taatcttcta cacgcacgtc ggcatcttcg ctgaagccgt
     9961 aagttgtggt ctgacgtccc acacgcggta acaattcgcg gatcaccgga tcatcaacac
    10021 acatcaccgc acgaccgtaa aaaggcaggt tgtgcagaaa attaataaag gtctgtttta
    10081 aattctcaaa gtcgccctgg taggtatcca tgtggtcggc ttcgatattg gtgacaatcg
    10141 ccaccatcgg ttgcagatgc aggaacgatg catcactctc atctgcttcg gcaatcaggt
    10201 accgaccatg ccccaaacgc gcatgaaccc ccgccgcttt taccagcccg ccgttaacga
    10261 aggttgggtc gagccccgct tctgcgtaga tgctggaaac catcgcggta gttgtcgttt
    10321 tgccgtgcgt tcctgcaatg gcgatgccat gacgaaaacg cattaactca gccagcattt
    10381 cggcacgacg gatcaccgga atacgcgctt catgagctgc gacaatttcc gggttatcgg
    10441 cagaaatcgc gctggaaaca acaaccacgc tggcatcacg tacgttttcc gggcgatggt
    10501 tgaaataaat cgtcgctccc agattcatta actgctgcgt gaccggattt ggcgctaaat
    10561 cggaaccact gatctgataa ccttcattgg ccagaacttc ggcaataccg cccataccgg
    10621 caccaccgat gccgacaaaa tgtatgtgcc gaacgcgacg catttcgggc acgatggaac
    10681 gcagttttgc caattgttgt gtattcattc tttacgccat taacttctta aattcatacg
    10741 atgcaaaaga catcgctaca attacgcccg ggcagcccgg ctcacttcat ttgccactcg
    10801 ctcggtggca tccggaatgg atgcagcgcg ggcgcgttct gccatggtta ataaggtttc
    10861 tcgcgaccac ccggccaggg tgttggcgac agcatccacg ctaagcagtg gctgctcgat
    10921 aattttggct gcgcccgctt tttccagcgg tagcgcattc cagtattgct ggcggtcttt
    10981 atgttgaaac ggcacaaaca acgccggtag tcctgccgcg gcgatttcac tcaccgttaa
    11041 agcaccggag cggcaaacga cgacatccgc ccacgcatac gccgccgcca tatcatcaat
    11101 aaattcagtc actttatgct gcggttgccc cgcttcggca tacgcctgtt caacggattg
    11161 ttgcgaacct ttgccgctct gatgccagat agtgactgaa tcacccagtt tcgcagcaac
    11221 ctgcggcatt gtctggttaa gaatgcgtgc gccctgggaa ccacccacta ccagcacacg
    11281 aaccggacct tcacgtccag ccaaacgttg ctgcggcaac ggtagcgcca acacatcggt
    11341 acgcaccggg ttacccacca cttccgcatt ggggaaagca cctggaaacg cctgcatcac
    11401 tttggtggca atcttcgcca gccatttatt ggttaagccc gcaataccgt tttgttcatg
    11461 aagtacaacc ggaatgccta acgaccacgc ggccagacca cctggacctg atacatagcc
    11521 gcccataccg agtaccacgt caggtttgta cgctttcata atcgcccgcg cctgacgcca
    11581 ggcgttgaag atacgcagcg gcgcagctat cagtgctttt ataccttttc cacgcagacc
    11641 agagatacga atgaaatcaa tttcgatgcc atgttttggc actaagtccg cttccatacg
    11701 gtcggcagtc cccagccagc gaacttgcca accctgagcc attagatggt gcgcaaccgc
    11761 cagtcccggg aatacatgtc caccggttcc gcctgccatc accattaatc gctttccttg
    11821 accactcatc gtgaacctcg tacaaacgcc tgcgctttct ccagacgcgt ttcataatca
    11881 atacgcaaca gcatcatgat ggctgtcgac ataatcagta agctcgaacc accgtaactg
    11941 atcagcggca atgtcagacc tttggtcggt aacatccccg ccgccgcgcc tacgttaacc
    12001 agcgcctgga agctaaacca gatgccaata gaacaggcga gaaaaccgga aaaacggtgg
    12061 tcaatttcta acgctttgcg gccaatcgac atcgcgcgaa aagcgacgaa gaataccatt
    12121 aaaagtgcca gcaccacacc gacatacccc agttcttcgc cgataatggc gaaaataaag
    12181 tcagtgtgcg cttccggcag atactccagt ttttgtaccg agttacctaa accttgtccc
    12241 caaagttcgc cgcgaccaaa cgccatcagc gattgcgtta actgatagcc gctgccaaag
    12301 ggatcttccc acgggttcca gaatgcggta acacggcgga tacggtacgg ttcggcgagt
    12361 atcagcaaca caaccgctga aatgcccata ccgataatgg caatgaactg ccacaatttc
    12421 gctcccgcca ggaacaacat cgccagcgta gtcacaaaca acaccaccac cgtaccaagg
    12481 tctggctgtg ccagcagtaa cactgccaac accagaatca cgcccatcgg tttcaggaag
    12541 ccgcgcaggt tattacgtac ttcgtcgcct ttacgcacca gatagttggc gatataacaa
    12601 aacagcgaca gttttgtcag ctccgcaggc tggatacgca gcaaaccgag atcgatccaa
    12661 cgcgatgccc ctttaaccga gctacccact accagcacga tcatcagcag gatgatagat
    12721 ccgagcagca tcgtggcact gtagcgttgc cagaactcca tcggcagacg cagcgtaatg
    12781 atcgccagaa tgaatgccag gatcagatag acgccatcac gcttcgcgaa gaagaacgga
    12841 tcgttggtta agcgttgccc tatgggcatt gacgccgagg tcaccatgat aaagccaatc
    12901 gccgcaaggc cgaaggtcag ccacagtaag gtgcgatcgt acatgatcag gctgtcggta
    12961 tctttttccc gcgagcccat cacccagcct tttagcgccg tggagatcca gaccaggata
    13021 ctgaatcctg gcaggcgcgg cattttcagg cgagggagag ataaacgcat caacctaact
    13081 ccttcgccag acgggcaaac tcattgcctc gttgttcaaa gttcttgaac tgatcaaggc
    13141 tggcacaggc cggggagagc agaaccatat cgcccggctg aacacgcgga gccagcaagc
    13201 gcatcgcctg ttccatagtt tcggtttgtt ctgccacttc cgggcgtagc gccgccagct
    13261 gcgcaccgtc acgaccaaaa cagtacaaac gcacgttgtc gccattcaga tagcgcgcca
    13321 gtggactgaa gtccgccgat ttaccatcgc cgcccagcaa caaatgcagc gtgccgtcta
    13381 cctgcaagcc attcaacgcc gcttccgtac tgccgacgtt agtggctttc gaatcgttaa
    13441 tccagcgtac gccattgtgc tccagcacca cttcaaagcg atgcggcaga ccggtgaagg
    13501 tggttaacgc tttcaggctg ctggcgcgcg gcaaaccagc tgcgtctgcc agtgccagtg
    13561 ctgccagcgc attggtgtag ttatgctgcc cggaaagttt catctctttc acattcagca
    13621 ctttctcgcc tttaacccgc agccaggttt cgccctgctg atgattcagg tgatagtcac
    13681 ccatgttgac gccaaagctg acgcagcgtt catccgcacc gcgaatcggc attgttaagg
    13741 catcatcagc attaaccacg caaactttcg cgttttcgta aatgcgcagt tttgctgcac
    13801 gatactgttg taaaccaaac ggatagcgat ccatatgatc ttcagtcacg ttcagaatgg
    13861 tcgctgctac cgcctgtaag ctggaggtgg tttccagctg gaagctcgac agttccagca
    13921 cgtacagttc gcactcagca tcaagcagca tcaacgctgg caggccaata ttgccaccca
    13981 caccaacgtt aacccccgcc gctttcgcca tttcacccac tagcgtggtg accgtgcttt
    14041 tgccgttaga accggtaatc gccacaatcg gtgcttgtgc ttcgcgacag aacagctcga
    14101 tatcgccaac gatttcgatt ccggcatcag cggcagcgct taaggatgga tgcgccagtg
    14161 caataccggg actggcgaca atcagatctg ccgccatcag ccattcatca ttcagactgc
    14221 ccgtgtggcg ttctacggct tcgggtaatt tatccaggcc aggcggtgtc atacgcgtat
    14281 ccataacgcg cggcgtcaca ccgcgagcga ggaaaaagtc cacgcaggaa agcccagtga
    14341 ggcccaggcc gataatgacg acatttttac cctgataatc agccatgatt aacgtacctt
    14401 cagcgttgcc agaccaatca gaaccagcat cagcgaaata atccagaaac gcacaatgac
    14461 gcgcggttcc ggccagcctt tcagttcata gtggtgatga atcggtgcca tgcggaaaat
    14521 acgttgtccg cgcagtttaa aggagccgac ctgcaggatg acagaaagcg tttctaccac
    14581 gaacacgccc cccataatca ccagcaggaa ttcctgacgt agcagtacgg cgataatgcc
    14641 taacgcacca cctaacgcca gcgaacctac atcgcccata aagacctgcg ccggataggt
    14701 gttaaaccac aggaagccca gtcctgcccc gactatcgcg gtacagacaa taaccagttc
    14761 ccctgcgtgt cgcagatacg gtatatgcaa gtagctggca aagttcatat tgccggtcgc
    14821 ccacgccacc agcgcaaaac caccggcgac aaatacggtc ggcataattg ccaggccgtc
    14881 gagaccatcg gtcaggttta ccgcgttgcc agtacccaca atgacgaagt aagccagcag
    14941 aatgtagaac agccccagct gcggcatcac atctttaaag aatgggacca ccagctgcgt
    15001 tgcgggcgtg tctttgccgg caaggtacag ggcgaaggcg acacccagcg caatgaccga
    15061 catccagaaa tacttccaac gagcgatcaa ccctttggtg tctttacgca ccactttgcg
    15121 ataatcatca acaaagccaa taacaccgta acctaccagc accaccaaca cgcaccagac
    15181 gtacggattg gacgggtaag cccacagcag tacggagatc acaatcgccg tcaggatcat
    15241 aatcccgccc atggtcggcg taccgcgctt gctgaagtgt gattcaggac cgtcgttacg
    15301 caccacctga ccaaaggaaa gtttttgcaa atgagcaatc atacgcgggc ccatccacaa
    15361 tgagatgaac agcgcggtca gcaggctgac gatggcgcga aacgtcagat aggaaaagac
    15421 gttaaagccg gaataatatt tgaccaaatg ttcggccagc caaactaaca tgtcccattc
    15481 tcctgtaaag cgcgtactac ctcttccatg gcggcactac gtgaaccctt aactaaaatc
    15541 gtaattaact gttgctcagc aatcagtgat ttaagacgcg taattaacgc agttttatca
    15601 gcaaaatgtt cgccaacgcc gctggcggtg ctgatagcat ggctttgttt acccacgctt
    15661 aacacgcggt caataccagc agcttttgcc gcctcgccca cctgtacatg gcaggcttcg
    15721 ctttcagcgc ccagttccgc catatcgccc accaccagca cgcggtagcc cggcatttca
    15781 gccagtacct gaacagcggc ggtcattgaa ccgacattgg cgttgtagga gtcgtcgagc
    15841 agcaactggt tttctgccag ttggatgggg aacagacggc ctggaacagc tttcagattt
    15901 gccagccccg ctttgatagc atcaagcgtt gcgcccacgg acatggagag cgcagcggct
    15961 gccagcgcat tcgcaatatt gtgacgcccc ggcaacggca gcagaacatc gacgctaccg
    16021 gttggggttt gtagggtaaa ttccgtaccg tgcgaggtca catggatatt ggtggcggtg
    16081 aaatcgctgt tggcggcatt aggtgagaaa cgccacactt tgcgtgagcc aattacgctc
    16141 tgccagttca gccagtcgtt gttgtcggcg ttcataatgg cgataccgtt ttccggcagg
    16201 ccgctaaaga tttcaccttt cgctttcgcg acacccgcaa gcgagccaaa accttccaga
    16261 tgcgccgctg ccaggttgtt gaccagcgca gcttccgggc gagtcagact cacagtccag
    16321 gctatttcgc cctgatggtt cgcgccaagt tcaataactg cgtaatcgta ttccggcgtt
    16381 aagcgcaaca acgtcatcgg tacaccgatg tcgttgttga gattgcctgc cgtataaagc
    16441 gtgttgccgc actggcttaa aatcgccgcc gtcatctctt taacggaggt tttgccggag
    16501 gaccccgtca gagcaaccac gcgcgccgga acttgctggc gaacccatgc agccagttca
    16561 ccaaacgcca gacgcgtatc cttgacgatt aactgcggca ggtcgatgtc cagcggacgg
    16621 ctaaccagta gtgcgcctgc gccgccagct ttcgcctggt cggcaaaatc gtgggcatca
    16681 aaacgttcgc ctttcagggc aacaaacagg cagcccggcg tcagttttcg ggtatcagtg
    16741 gttacagcat caagggtgat atctgcacct tgcagttcac cgttgagaat gtcggtaagt
    16801 tggctaaggg ttacgctaat catgcaatca cccccagcag acgcgccacc gtgacgcgat
    16861 cggagtagtc cagacgctga ttgccaacaa tctggtaatc ttcatggcct ttgcccgcga
    16921 ccagtaccac atcattctct ttagcctgca taacggcgca agtcaccgct tcagcacggc
    16981 cttccatcac tttggcatgt ccggcatcta acattcccgc cagaatatcg ttgatgatgg
    17041 cacgcggttc ttcggtacgc gggttatcgt ccgtcaccac cgccacgtca gcaaactctt
    17101 cggcaattgc gcccatcagt ggacgcttac ctttatcgcg atcgccacca cagccaaaga
    17161 cgcaccacag cttgcccgca cagtgcaggc gcgccgcctg taaggctttt tccagtgcat
    17221 ccggcgtatg cgcgtaatcc accaccaccg tcggtttgcc tggcgcagtg aacacttcca
    17281 tacgtccgca aaccggttgc agacgcgcgg cggttttcag cagatcagcc agtggatagc
    17341 cgagtgccag cagtgtcgcc agcgcgagca gcaggttgct gacgttaaaa gcgcccatca
    17401 gatggctttc aatttcgcca tcgccccaac ttgagctaaa gcgaatcgtc gcaccgctgt
    17461 cgtgatagtt cacgtcgatc gcttttaacc agcgtccgtg acagttcgga ttgatatgat
    17521 cttccattga taccgcaacc gcgtccggca gttttgccag ccagcggcgg cccacttcat
    17581 cgtcggcgtt aataatcgcc tgaccgcaat gatgttcaga ataaagcagc catttcgcag
    17641 cttcgtagtg ttccatatca ccatgataat caaggtgatc gcggcttaag ttggtaaaga
    17701 ccgacgccgc aaatttcaat gccgccacac ggtgctgtac cagcccgtgg gaggaaactt
    17761 ccattgcgca aaacgtcgcg ccctgctcca ccagccccgc cagctcatgc tgaacatcga
    17821 ctgccgaacc ggtagtattt tctgtcggga tcactttccc aagcagaccg ttaccaacgg
    17881 tgcccattac cgcgctggtt tcgccaagca gttggctcca ctgcgccagc agctgggtag
    17941 tcgtggtttt gccgttggtg cccgttacgc ctacgagacg taaattgtca gagggttcat
    18001 ggtaaaagcg gcccgccagt gcagataaac gctcgttgag ctggctgaga tagatgaccg
    18061 gtacgccgtg catttcacgg atttcaccgt cggtcgcctc atctttcgcc tctgcaataa
    18121 tggcagccac accttgcgct atcgcctgcg ggatatatcg acgcccgtcc gcctgatgac
    18181 ctactacagc tacaaagaga tcgcccgccg cagccacacg gctgtcgagt gtcatctctc
    18241 gcagtgctcg cgaaggtgcg tctggcaccc acggagcaag aaggtcgcgc aaattacgat
    18301 ctgccacctg tcccctcgcc ttgattaatc acaaattcat ttttatcgcc cgttgtcagc
    18361 gcatccggct cgatgttcat ggtacgcaat acgccgccca tgatggcacc aaagaccggc
    18421 gcggaaacgg cgccgccgta gtatttaccc gcctgcggat cgttgataac aacaaccagc
    18481 gcgaagcgcg gctgactcgc aggcgcaacg cctgcggtat aagcaatata tttattgatg
    18541 tagcgaccgt ccggcccgac ctttttcgcg gtaccggttt taatggcgat acgatagcct
    18601 ttaatcgccg ctttcacgcc gccgccgcct ggtagcgcca cgctttccat catatgcacc
    18661 acggtgcgaa caatggattc cgggaagaca cgttcaccgg gaaccggggg gtcaactttg
    18721 gtaatcgaca gtgggcgata aatgccgtag ctgccgatag ttgcgtagac tcgcgctaac
    18781 tgtaacggtg ttaccattag cccgtagccg aaagagaagg tggccctctc tatgtcagac
    18841 caccgttgtt tttgaggata taagccactg cgttctccga ccaaccccaa attggtcgct
    18901 tttcccagtc caaaacgtga gtaagtatct actaacgctg aggacggcat cgctaacgcc
    18961 agcttggaaa caccgacgtt actcgacttc tgtaataccc cggtcagggt taattcgctg
    19021 tagcgtgcca cgtctttgat ttcgtggccg ttaattcgat aaggaatggt attgagtacc
    19081 gagttttccc gcaccacgcc acgttgcaac gcggtcatta ccaccatcgg tttaaccgtt
    19141 gagcccggtt caaacacgtc ggtgatggta cggttacgca tcgcctcttt cggcgtgccg
    19201 ctcagattgt tagggttgta tgacgggctg ttagccatcg ccagcacttc accggtgttg
    19261 acatccacca gcacggcgct accggattca gccttgttaa aggccaccgc gttgttcagt
    19321 tcgcgataaa ccagcgcctg caagcgttca tcaatactca acgccaggtt gtgcgccgcc
    19381 tggctgtcag tagaagaaat atcttcaatt acgcgaccat agcggtcttt acgcacaatg
    19441 cgctcacccg gctgcccggt aagccattta tcgaaactct tctcaacgcc ctcaatccct
    19501 tgactatcga cgttagtaaa gccaatgagg tgagcagtca cttcgccgga tggataataa
    19561 cggcgagatt cttcacgcag atgaatcccc ggcagtttca gttttttgat gtagtccgcc
    19621 atgtcagggt ttacctgacg cgccagataa ataaagcgcc ctttcgggtt ggcgttaatg
    19681 cgggctgaaa gctgatccag cggaatattg agcgcgttag ccagcgcctt ccagcggtca
    19741 ccgacgctga taccgccagc gtcatgcact tctttcgggt cagcccaaat cgcttttacc
    19801 ggcacgctca ccgctaacgg gcgaccagaa cggtcagtaa tcatgccgcg ggaggtggaa
    19861 acttgctgaa cgcgaagaga acgcatgtcg ccctctttca ccagcatatc cggggagata
    19921 acttgtaacc acgctacgcg tccgagcaga aaagccagcg ccaggagaat acagccgcat
    19981 aacaacgcaa aacgccaact gataaagttg gcatgttctt cctgacgttt tggtttctgc
    20041 gttttcgccg ctgctttcat gcgtcgcgtt tatccttatt tttgcactac gatattttct
    20101 tgtgacggat caacatgctg catttgcagt ttttccgtgg cgatcctttc cacccggcta
    20161 tggtcgccga gcgcattctc ttcaaggatc aggttgcgcc attcaatgtc taaagcatct
    20221 cgctccagca ccagttgttc gcgctgagcg gtcagtaaac gggtatggtg cgccgtggtt
    20281 accacggtca ccgccgtcaa aataatgcaa atgaacaggc agagtggcag cttcccaaat
    20341 cgcaaaagat cgtcaccgat aacaccaggc aatgcatggc gctcgtggct tcccatcgat
    20401 cctttaactt tgcttagagc ttctgtcact ctgctgatca tgcgttcgtc ctctctgcaa
    20461 tacgcagaac tgaactacgg gcacgaggat tctcagccac ctcttcttcg cccggcatta
    20521 acttgcctaa tgctcgcagc tgacggccac ccagtttttt gagctgctct tcagtcatcg
    20581 gtaaccctgc cggaacttgc ggaccgcggc tgttttcacg cataaaacgt ttcacaatac
    20641 ggtcttccag cgagtggaag ctgatgatcg aaagccgccc acccggggcc agcacgttga
    20701 gcgagctttt tagcgcctgc tctatctcct ccagttcact gtttacccaa atgcgcaccg
    20761 cctggaaggt acgggtcgcg ggatgtttga atttgtcttt caccggcgtt gcggcagcca
    20821 cgacttccgc cagttctttg gtgcgggtca tcggctgttc gcggttacgc tcgacaatgg
    20881 cgcgggcaat gcgtttggca aaacgctctt caccataggt tttcaatacc caggcgatat
    20941 cggcttcttc tgcggtttgc agccattcag cggctgactg cccacgggtt gggtccatac
    21001 gcatgtccag cggaccatcg cgcataaagg aaaagccgcg ctcagcatca tcaagttgcg
    21061 gtgaagagac gccaagatcg aggagaatgc cgtcgatctt gccgataaga tcgcgctcgg
    21121 caacgtattc gcccagcgcg gagaaaggtc cgtggatgat ggagaagcgc ggatcatcaa
    21181 tagtcttcgc aacggcgata gcctgcgggt cgcgatcgat cgccagcaaa cgcccctctt
    21241 cgccaagctg cgagaggatc agacgtgagt gaccaccgcg accaaaagtc ccatcaatgt
    21301 agatgccatc aggacggata ttgaggccat taacggcttc atccagcagc accgtagtat
    21361 gtttatagtt ttccatcatt ttatagagac aagtcctgca gtcgctccga taagtctccg
    21421 gtagccaact gctctgcgtc gatatcttcc ttgacctgtt gatgccaggt tgtttcatcc
    21481 cacagctcaa acttgttgaa ctgtccaacc agcatcactt cttttgtcag cccggcatgt
    21541 tgccgcagta ctggcgcgat taacaatcga cctgcgccat ccatctgaca ttcgctggca
    21601 tgacctaaca gtaggcgctg cacacggcgc tcaaccgggt tcatgctcga cagacgcgat
    21661 aatttttgct cgataatttc ccattcaggc agggggtaaa gcagcaggca cgggtgatga
    21721 atgtcaatgg tgcaaaccat ttgaccggca gcgttctcaa gcagctgttc ccggtaacgg
    21781 gtaggcactg ataagcgccc tttgctgtcg agattgacta acgttgctcc ccggaacatg
    21841 ccagcctcac cccttattcc cactttgccc cacaatttcc cactgaaagg agtttacgga
    21901 gcggaggaaa agcttgtcaa gccagtcaca aggcataccg aggtaaaaaa cccgatgttt
    21961 acggctaata tcagcgttgt ttattttacg tccaacgtgc gcagcaaatt aacgttatga
    22021 atagctgtaa gaaaaaatcc ctcatcgtca tcaccgtttt aaaatgactc actattttcc
    22081 acagcaaata taaagtgtca gtttgcgacg cgagcggcat tttaggacat atcttccccg
    22141 gttaacagtc cctgttgcgt ctgtgtggcg cttgatgcca gcgggtttgc gcaaaagcaa
    22201 agaaaagtgc ttgttaaatc ggcaattcat ctctgcatgt aacaaaataa tgcaaaaacg
    22261 cgctgtcgat atcacttaat tattgtgaat ttcagctggt gagttaatta acgggcgggt
    22321 ttaaaagaac ttatcaataa tagtcaggaa tagtcttatt tactttaagg atattgatgt
    22381 ccattcccgt actctacgcg ccagagggaa attcacctgg cgcgtattat tgttcgcggc
    22441 ttagctacgg ctgagcacac cgcggcgata gagattacgt ttaatgcgcg ttaaaccagg
    22501 ttttggctta cgcggttcgt ccaggcttgc caggacaatc tccagcacac gctctgcgac
    22561 atcgcggtga cgttgagcca ctgccagcac cgggcactgt aagaagtcga gcagttcgtt
    22621 atcgccaaag gtggcaattg caaggtcaga aggcagttta ccatcgcgac gtagcgtgac
    22681 atccatcacc ccttgcaaca acgcaaacga cgttgtgaac agcgcctgcg gcatcggatg
    22741 cgtttccagc catttttcga ataactgggc agccgcctcc cgctcatagc tgttggcata
    22801 caggaaatgc acttcgcgcg gatcatcttt ccaggcagta cggaaacctt gttcacgcag
    22861 gaagctgaca gaaagctccg gcagcgcgcc aagataaagc accgtctcgg cgggaaactt
    22921 acgtaactct tccgccagca tttcggcatc atcctgatcg gcaccaacca cgctggtgaa
    22981 atgttcacga tcgagggcgc ggtccagcgc gacaatcggg aacgggtcgt tagcccagcg
    23041 ttgataaaaa ggatgctcag gaggcaacga tgtcgaaaca ataatggcat cgacctgacg
    23101 ctgtaaaagg tgctcaatac agcgcatttc gttgtctggc tgatcttctg agcaggcaat
    23161 tagcagttga taaccccgtt gccgcgcctg gcgttcaaga tagttagcga tgcgggtata
    23221 gctggtgttc tccagatcgg ggatcacaag accaatagaa cgtgtgcgtc cagcacgaag
    23281 cccagctgcc acggcgttcg ggtggtaatt gtgctcacgc accacagcca tgactttttc
    23341 aacggttttg tcgctcacac ggtattgctt cgctttgccg ttaataacat agcttgcagt
    23401 ggtccgcgac actcccgcca gccgagcgat ttcatccagt ttcacaattg ccccttgcgt
    23461 aaaatgtaaa aaccatagcc attgtactgg catgggttaa attcattaac atctaagcgc
    23521 agaaaattaa ctgcggcaac tgcttttatt ccaggttccc actgatttcg caaaaaaagc
    23581 ccaacgtgac atgttgggct gcaaattgcg cattcagatc attctcaacg cattatttta
    23641 tcgccgcgcg aaagtccgac cacaccagaa cgagcaactt ccacaatttt cgccacctcg
    23701 cgaatcgatg ctaaaaatgc atcaagctta tcgctggtgc ctgctaattg aacggtataa
    23761 agcgagggtg tgacatcgat aatttgccca cggaatattt ccgtattacg tttcacttcg
    23821 tcacgcccgt aaccgctggc ctgaattttc accagcatga tttcccgctc aacatgcgcg
    23881 ccctgcccca actcgctcac gcgcaagaca tcgaccagct tgtgtaattg cttttcgatc
    23941 tgctcaagca ctttttcatc gcccacggtc tggatggtca tacgcgataa tgtcggatcg
    24001 tcggttggcg caacggtcag gctttcaatg ttgtagccac gctgggaaaa aaggccaatc
    24061 acgcgggata acgcgcctga ttcattttcg agtaagactg ataatatccg gcgcataatc
    24121 aggttctctc cgttttgctt aaccacattt catccattcc gcccccgcga atctgcatcg
    24181 ggtagacgtg ctcactgcca tcgacggtaa catcaacaaa caccaggcga ttattgcgca
    24241 cctgttccag cgcctcgcta agtttgcttt ccagctcatg cggatgagag atctggatcc
    24301 cgacgtgacc ataggcttcc gccagacgga cgaaatcggg tagtgattgc atataagatt
    24361 gtgaatgacg gccggaatag atcatgtcct gccactgttt caccatcccc agatagcggt
    24421 tattgagatt caccaccagt acgggcaact cgtattgcaa cgcggtagac agttcctgga
    24481 tgttcatctg aatactgccg tcgccagtga cgcaaaccac ggtttcttct ggcaacgcca
    24541 ttttgacgcc cagtgccgca ggtaaaccaa aacccatcgt gccgaggcca ccggaattga
    24601 tccagcgacg cggtttgtcg aatggataat aaagtgcagc aaacatctgg tgctgcccga
    24661 catcggacgt cacgtaagcg tccccattag tcaaccgcca aagagtctcg atcaccgcct
    24721 gcggtttaat cttttcactg tgagtgtcat atttcaggca ctgacgagcg cgccactgtt
    24781 caatttgctg ccaccagtcg cggatctcat ccagtggttg atgggcggat tcttgcgaca
    24841 ggagttcaag catttgttcg aggacctggc gagcatcccc cacaatcggg atatccgcag
    24901 tcacggtttt agaaatggaa gtaggatcaa tatcgatatg cagaacagtg gcatttgggc
    24961 agtactttgc cagattgttc gtcgttcggt catcaaatcg taccccgacg gcgaaaatca
    25021 catccgcgtt atgcatcgtc atattggctt cgtaggtacc gtgcattccc agcatgccca
    25081 gtgcctgacg atgcgttgcc ggaaacgccc ccagccccat caatgaggaa acaacgggca
    25141 gattcaacgc ctccaccgtt tctttcaact gctgatggca gcccgccgtg attgccccac
    25201 cgcctacgta gacaaccggt ttttttgccg ctaccagcgt ttgcagagca cccttaattt
    25261 gccctttatg tccggtagta gtgggattgt aagaacgcat actgaccgac tcaggccaga
    25321 catagggtaa tttgttcgcc ggattaagaa tatctttcgg taaatcaacg accacgggtc
    25381 ctgggcgacc acttgccgcc agccagaaag cctttttcag cacctgcgga atgtcttccg
    25441 tttgcttaac cagaaaactg tgtttaacca ccggtcgcga aatccccacc atgtcgcact
    25501 cctgaaaggc atcgtaacct atcaacgagg tcgctacctg cccggaaagg acaactaatg
    25561 gaatggaatc catataagcg gtggcgatgc cggtaatcgc attggtcgcc cctggacccg
    25621 acgttaccag cacgacgccg acttccccgg tcgcacgcgc caggccatcg gccatatgca
    25681 ccgccgcctg ctcatgacga actaatacat gatcaatacc acccacggta tgcaatgcat
    25741 cgtaaatatc aaggactgcg cctccgggat aaccgaatac ttgtttaacg ccctgatcga
    25801 taagcgatcg gacgaccatc tcggctccag acaacatctc catggcctgc ctcactgttt
    25861 gacggaaaaa atgtgtaaaa cacattaacc gtccgatctt gcttaagcaa ttggcagcca
    25921 gagacataaa cgcagggtaa taagaataaa caggaggaaa ggtgaaaaga aacgataaaa
    25981 tcctccatta cgaaggatta aattgataat aaatcggctg aatcccacaa cttacagcaa
    26041 tttctctatt tttttaatta atcctaaaat agagagtcca cacattcagg ataaaattct
    26101 acattcaata aaaaatgcag aataaaccag acattcatgt ctggcctatt ctgcattcag
    26161 ttagcgtttg caaattgaga ctaattgttc ttccatccac tgatggcctt tatcgcgccc
    26221 ggcagcttca tgccaggaga gataacaggt tctgctgttt tgttttaacg gcagcggtaa
    26281 tacctgtaat tctaaggatt cagcgaactc ttcagccagc caacgcggcg caatagcgac
    26341 caaatgcgtt tgcgacacca cgctaagtac gctcatcatt gccatgccct gatacgcgat
    26401 actggcttgc ttatctaccg tgtcatacca aggttgacta aatgacgcga aacgatcgag
    26461 cgaaaccgcc gcatgttgtt cgttataaac atcatgtttc agtaacgggc ctttaattgt
    26521 tggatgattt ttgctggcta ccagcaccat ttcatcttta aataatggca cgctggtaaa
    26581 ttcaggacga tggaagtctt cataactaat cacaaactcc gtttcctgat aacgcagctg
    26641 atgttcagtg ttctgattta atgaagactt gaacataaca tgtatatttg gcgcaatctg
    26701 ctcaatgtga ttataaatct gcgaggtcag aatgctgtct aacgggctgc aaacacaaag
    26761 atgaaataca cgttcactgc tcgcgggttc aaaacctgaa ccaggcaatt cattttgtac
    26821 tagttgcaat gcctgacgaa ctgagccaaa aagttgaaat gcgcgagcag tcggttgaat
    26881 accacggcca taacgaacaa aaagctcgtc attaaacatc accttcaggc gtgcaacagc
    26941 gttactgacc gcaggttgcg acatacccag aacatgagcg gcacgggtaa tgttttgctc
    27001 ctgcatcacg gcatcgaaaa cggttaataa gttgagatcg accatgcgta gctgtggctt
    27061 gcttaactcc gccgtctctg aatgatctgt ttgtacctct ggcatactta actccactgt
    27121 cacacttaac tccctttccc ttattggaat gcagagaaat aatcctgaag aatatgattt
    27181 atcatgcata taaaataaga aaaagcaaaa tgagtaaaat tcggaaaaca taaagatacg
    27241 ggttctgata aaaacagaat ccattgccgt ggaatgagtc atttaccaca gcataataat
    27301 ccataatgtt tttgttgcga aaacaatcta atc
//
LOCUS       DAZGVQ010000060        27153 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000060 DAZGVQ010000000
VERSION     DAZGVQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27153
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.060"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..252)
                     /locus_tag="ACHLAG_002404"
     CDS             complement(<1..252)
                     /locus_tag="ACHLAG_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0655619.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTSAERQRLKELERENRELR"
     gene            441..1025
                     /locus_tag="ACHLAG_002405"
     CDS             441..1025
                     /locus_tag="ACHLAG_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655620.1"
                     /translation="MGKMKELLFEIQEERTDEWIAENYTDAEEGTPEWDAAAQEYSWF
                     QDWMEEAAEQQYFEASLASIPDRLQDAKDELFELENLMQFNQPGIVERMAYVHCVSVL
                     DSFLMYSARALLNHPPHLQRFLQVADSLIANKEDRRKLRASKWVEQEPDTATPEKVYT
                     WRAQSLVAKKTFQSHKVIDRYFSTMLATPHEWPL"
     gene            1268..1789
                     /locus_tag="ACHLAG_002406"
                     /pseudo
     CDS             1268..1789
                     /locus_tag="ACHLAG_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001495932.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            1970..2614
                     /locus_tag="ACHLAG_002407"
     CDS             1970..2614
                     /locus_tag="ACHLAG_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP0655621.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            2701..3009
                     /locus_tag="ACHLAG_002408"
     CDS             2701..3009
                     /locus_tag="ACHLAG_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HFP0655622.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATVPSQPNEGTEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            3423..4403
                     /locus_tag="ACHLAG_002409"
     CDS             3423..4403
                     /locus_tag="ACHLAG_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFP0655623.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPIDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMREVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            4396..4812
                     /locus_tag="ACHLAG_002410"
     CDS             4396..4812
                     /locus_tag="ACHLAG_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFP0655624.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(4814..5781)
                     /gene="umuC"
                     /locus_tag="ACHLAG_002411"
                     /pseudo
     CDS             complement(4814..5781)
                     /gene="umuC"
                     /locus_tag="ACHLAG_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
     gene            complement(5771..6190)
                     /gene="umuD"
                     /locus_tag="ACHLAG_002412"
     CDS             complement(5771..6190)
                     /gene="umuD"
                     /locus_tag="ACHLAG_002412"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFP0655625.1"
                     /translation="MSTVYHRPADPSGNDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVVH"
     gene            complement(6187..6435)
                     /locus_tag="ACHLAG_002413"
     CDS             complement(6187..6435)
                     /locus_tag="ACHLAG_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP0655626.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIERRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            7458..9182
                     /gene="ltrA"
                     /locus_tag="ACHLAG_002414"
     CDS             7458..9182
                     /gene="ltrA"
                     /locus_tag="ACHLAG_002414"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP0655627.1"
                     /translation="MTEQATTCKGASLLNGDSWHSINWRQCYREVRRLQARIVKATRE
                     GKHGKVKSLQWILTHSFSGRAVAVRRVTENSGKRTPGVDGQTWSSPEVKFLAINLLKR
                     RGYKPQPLKRVYIPKSNGKSRPLGIPTMKDRAMQALYLLALEPVAEVTADQRSFGFRT
                     GRSTADAIAQCFCVLAQKTSAEWVLEGDIRGCFDNISHQWLIDNTSTDRQILTKWLKA
                     GYREKGQLFPVNSGTPQGGIISPVLANIALDGLEALLASEFKKRTVKGRLVNPKVNYV
                     RYADDFIITGESKELLESQVLPVVRRFMAERGLMLSPEKTKITHIEEGFDFLGQNIRK
                     YGGKMLIKPSKANVSSFLKKIRAVIKGNKAMDQLTLIRMLNPMIKGWAAYHQHIVAKV
                     AFNKVDNEIWLALWRWAVRRHPNKGKKWIRKRYFHQQGARNWSFSTATGELLANGKPQ
                     YANLRKAIDTPINRFKPIKIAANPFDPQWEMYFEERCADKMRHKLQGRKKLIQIWFEQ
                     RGRCPICDERITSDSQWQVHHIIRRVDGGSNCLSNLIMLHPMCHTLVHAKGIHVVKPA
                     HESGLRKA"
     gene            9259..10161
                     /locus_tag="ACHLAG_002415"
     CDS             9259..10161
                     /locus_tag="ACHLAG_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFP0655628.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVVAELSRRFGCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            10158..10469
                     /locus_tag="ACHLAG_002416"
     CDS             10158..10469
                     /locus_tag="ACHLAG_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655629.1"
                     /translation="MKYDGDGRATARFFSGKGCRHAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGITLPSNRRRLRYSTVGLTRYRT
                     R"
     gene            10545..11228
                     /locus_tag="ACHLAG_002417"
     CDS             10545..11228
                     /locus_tag="ACHLAG_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFP0655630.1"
                     /translation="MSRFVLGNCIDVIARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFVAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLSAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            11229..11450
                     /locus_tag="ACHLAG_002418"
     CDS             11229..11450
                     /locus_tag="ACHLAG_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655631.1"
                     /translation="MHYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDACFKD"
     gene            11431..11898
                     /locus_tag="ACHLAG_002419"
     CDS             11431..11898
                     /locus_tag="ACHLAG_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFP0655632.1"
                     /translation="MRALKIEKETRMYGTCETLCRELAAKYPGDTPLMLVVWSPEEIQ
                     ALADGMDISLSDHEIRTVLARLEDIPEDQRIESGISSAAAMEIISNVSENRQVTVPAE
                     LLASLIQTAEQALWKREWAARDYGLAVPECVTRRQAVVNQARILLKNNTHEND"
     gene            11943..12713
                     /locus_tag="ACHLAG_002420"
     CDS             11943..12713
                     /locus_tag="ACHLAG_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655633.1"
                     /translation="MNKTLNALICRHARDLLLAQGWPEETDVDQRNLKYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRNAVRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDYPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVCECVEHILASGLVCDVLR
                     IPDEPSRRWFDRDILREVVLEARDEIRSMADALAKIRG"
     gene            13127..13552
                     /locus_tag="ACHLAG_002421"
     CDS             13127..13552
                     /locus_tag="ACHLAG_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0655634.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            13552..14043
                     /locus_tag="ACHLAG_002422"
     CDS             13552..14043
                     /locus_tag="ACHLAG_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFP0655635.1"
                     /translation="MNGRGGNAAPVKKTRTCTVKEIIRDVLDTDVPDSECVLAVVLTR
                     GDVRHMAQDWNLSDDELETVMQRLDDAFEYGADVSIVHDVVRELMEEKRASRQVTVPA
                     VMLEKVMALAGSEMKRLYAVGSENGGDGDAFVREEREAMDVVLQGQAVEAVCQPARPP
                     LAG"
     gene            14964..15194
                     /locus_tag="ACHLAG_002423"
     CDS             14964..15194
                     /locus_tag="ACHLAG_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655636.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            15246..16607
                     /locus_tag="ACHLAG_002424"
     CDS             15246..16607
                     /locus_tag="ACHLAG_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HFP0655637.1"
                     /translation="MTSAAITMTAPEAASPVQMYRATYSPDDNKLRLYAVSRLDPETY
                     KKVHDAGFRWAPKQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVAEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            16655..17218
                     /locus_tag="ACHLAG_002425"
     CDS             16655..17218
                     /locus_tag="ACHLAG_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFP0655638.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVTPAPVCDRLVM
                     LAEISNRDHILEPSAGTGAILRAIRDTAPGAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQPVQYYSRVIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            17218..17460
                     /locus_tag="ACHLAG_002426"
     CDS             17218..17460
                     /locus_tag="ACHLAG_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655639.1"
                     /translation="MTQGRREDKCLSARAGGAERGRAHAPPLSLRGRSDGGFCFFMPV
                     ICLLLMMLLFLLLLLLLLLLLLLLLLLLLFFFFLLL"
     gene            17352..17630
                     /locus_tag="ACHLAG_002427"
     CDS             17352..17630
                     /locus_tag="ACHLAG_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001366172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655640.1"
                     /translation="MSVTDDVVVPATATATATATATATATATVLLFPAALILTTAVLS
                     CLPGAPSAPGCLAASGRQARLRQAAAGAPASLRRTRWRGFGLSLRSDA"
     gene            17871..18077
                     /locus_tag="ACHLAG_002428"
     CDS             17871..18077
                     /locus_tag="ACHLAG_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0655641.1"
                     /translation="MYIPQNFLPKSRQKLLRSAPGQSFPSVAVMPLAAKVLKKVMVNT
                     GGNKRQVPAKRQNRGSRNSKDDGG"
     gene            18103..18642
                     /locus_tag="ACHLAG_002429"
     CDS             18103..18642
                     /locus_tag="ACHLAG_002429"
                     /inference="COORDINATES: protein motif:HMM:NF010292.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0655642.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGRAAGAQTQPEEGQQFSAQPQPEPQSEAGTKKGGAKTKGRGRKAAQPE
                     PQLQPPEGDDYGFSDDIPF"
     gene            18705..18938
                     /locus_tag="ACHLAG_002430"
     CDS             18705..18938
                     /locus_tag="ACHLAG_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFP0655643.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGAMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            19004..20962
                     /locus_tag="ACHLAG_002431"
     CDS             19004..20962
                     /locus_tag="ACHLAG_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP0655644.1"
                     /translation="MSVTESKAKTERKSSRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNTKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEYLREAEGWGWCAGRMEAVGECREDARAYRNLPQPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDVADDADHTEQMQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFGSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFMALMAEKARL
                     AALLPEGWTRDMTMFLSLSLEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEHHMKD
                     NRWVPGWMCAPHPQTDTTERTDNLADAA"
     gene            21017..21451
                     /gene="psiB"
                     /locus_tag="ACHLAG_002432"
     CDS             21017..21451
                     /gene="psiB"
                     /locus_tag="ACHLAG_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFP0655645.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            21448..22210
                     /locus_tag="ACHLAG_002433"
                     /pseudo
     CDS             21448..22210
                     /locus_tag="ACHLAG_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
     gene            complement(22179..22367)
                     /locus_tag="ACHLAG_002434"
     CDS             complement(22179..22367)
                     /locus_tag="ACHLAG_002434"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655646.1"
                     /translation="MKINLLRGYLLSATQHGNKPPSRHEAESLKRRAHHSPYTCTLTT
                     LTFPENNPLIQTVHGKSV"
     gene            22389..22538
                     /gene="mok"
                     /locus_tag="ACHLAG_002435"
                     /pseudo
     CDS             22389..22538
                     /gene="mok"
                     /locus_tag="ACHLAG_002435"
                     /inference="COORDINATES: protein motif:HMM:NF010277.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance protein Mok"
     gene            22480..22605
                     /locus_tag="ACHLAG_002436"
     CDS             22480..22605
                     /locus_tag="ACHLAG_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HFP0655647.1"
                     /translation="MLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK"
     gene            22825..23055
                     /locus_tag="ACHLAG_002437"
     CDS             22825..23055
                     /locus_tag="ACHLAG_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655648.1"
                     /translation="MACQKSHHLPCDYSLFLPALILTTAVLSCLPGAPSAPGCLAASG
                     RQARLRQAAAGAPVSLRQTRWRGFGLSLRSDA"
     gene            complement(23075..23212)
                     /locus_tag="ACHLAG_002438"
                     /pseudo
     CDS             complement(23075..23212)
                     /locus_tag="ACHLAG_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23282..23488
                     /locus_tag="ACHLAG_002439"
     CDS             23282..23488
                     /locus_tag="ACHLAG_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0655649.1"
                     /translation="MHIPQKFLPKSRQKLLRSAPGQSFPAVAVMPLAAKVLKQVMVNT
                     GGNKRQVPAKRQNRGSRNSTDDGG"
     gene            23513..23800
                     /locus_tag="ACHLAG_002440"
     CDS             23513..23800
                     /locus_tag="ACHLAG_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655650.1"
                     /translation="MSTRNIHVNTASYTLLVAGKKKNTGEEWDVLEFSSLTELKKYRK
                     SHPEKMAFSYSYALSRGVDTQFRHINIAEADHFKQFLRQIKRAGLDIRAIC"
     gene            23919..24740
                     /locus_tag="ACHLAG_002441"
                     /pseudo
     CDS             23919..24740
                     /locus_tag="ACHLAG_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
     gene            complement(25036..25626)
                     /locus_tag="ACHLAG_002442"
     CDS             complement(25036..25626)
                     /locus_tag="ACHLAG_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020246007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFP0655651.1"
                     /translation="MVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFINEIC
                     QATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQHFNEL
                     ARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQRRLA
                     YASDVYRIYTGIKSSKGIRIPATKKSLPEINSVQNN"
     gene            25978..26361
                     /gene="traM"
                     /locus_tag="ACHLAG_002443"
     CDS             25978..26361
                     /gene="traM"
                     /locus_tag="ACHLAG_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFP0655652.1"
                     /translation="MAKIQVYVNDHVSEKINAIAVQRRAEGAKEKDVSYSSIASMLLE
                     LGLRVYEAQMERKESAFNQAEFNKLLLECVVKTQSTVAKILGIESLSPHVSGKPKFEY
                     ANMVEDIREKVSVEMERFFPKNDDE"
     gene            26552..>27153
                     /gene="traJ"
                     /locus_tag="ACHLAG_002444"
     CDS             26552..>27153
                     /gene="traJ"
                     /locus_tag="ACHLAG_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HFP0655653.1"
                     /translation="MCAMDRRERALISQLHFPHSLGEAISFLKYPVCVRFEDGSFIQE
                     NNRFEKLIRSSFNSCDEWFDSLTLECKLQLSRAEIESCSSVYGVNCNNDILLNNMLWS
                     VIIESVITPCGYFFIWRFIWVANDNLSSFVVSKYTNELIVLSDEYVGIEPYLIGFSHH
                     YSSAKMNITVSKSKKKTMKLFKRHGFSSRDLWLDEMIRTE"
BASE COUNT         6493 a         6636 c         7521 g         6503 t
ORIGIN      
        1 gcgcagttca cgattttcac gttccagctc tttcagacgc tgacgttcag cgctggtgag
       61 cccaccatca ccgcccccgg tatcccgctc atgctggcga acccagacac gcagagtctc
      121 cggcgtacag ccaatctttg gggcaatgga acaaattgcc gcccactgtg agtcatattc
      181 gccctgactt tccagaacca tacgaactgc gcgttgacgg acttcggggg aaaaacgagt
      241 atttttagtc atcctgttta cctctttctc agggagttta gtctccagga tttccggggc
      301 ggttcatgct ttcgtgcggt atacctctgg ataaaaacat cgcatcacat tacgccaagc
      361 atggatactg aataaatcta atcctggaat gagcacagac aaatgccagg cgggatttca
      421 ggagattcag tgaggtataa atgggaaaaa tgaaagaact gctgtttgaa attcaggaag
      481 aacgcactga tgaatggatt gcggaaaatt acacggatgc tgaggaagga actcctgaat
      541 gggatgcggc agcacaggaa tacagctggt ttcaggactg gatggaagag gcggctgagc
      601 agcaatattt tgaggcttcg cttgccagta ttcctgaccg tctgcaggat gcaaaagatg
      661 aattgtttga gttagagaac ctgatgcagt tcaaccaacc gggaattgtg gagcgaatgg
      721 cttacgttca ttgtgtgagt gtgttggaca gttttctgat gtacagcgca agggcacttc
      781 ttaatcatcc tccacatttg cagcgatttc tgcaggtagc tgattcgctg attgctaata
      841 aggaagacag aagaaaatta cgtgcttcca aatgggtcga gcaggaaccg gatacagcca
      901 ctcctgagaa ggtttatacc tggcgagctc agtctcttgt ggcgaagaaa acgtttcaga
      961 gccacaaagt gatagatcgg tatttttcaa cgatgctggc aacaccgcat gagtggcctt
     1021 tgtaggaact tgagagggta atcaaggtcc gaaacgcact ggtacaccgt gatggagtta
     1081 ccgaatctct tgaacccgtg tatatcagtt cggggagtgt gcagaatgct atctgcacgg
     1141 tacgagcatt tattaccgcg gcggctgaaa cccttctgca ggaagatgcg ctatacagga
     1201 cggatgatgg cacgttctga aattaccgca gcgacaccat ccataagcct cctcttcact
     1261 gagggatatg tcgatggttc atgatgaact gccaaaatac ctgctggtca ctctgaagca
     1321 gcggacagaa aaagcagcca ggacggcagg aagaacgcca gccgggcagt agactcaccg
     1381 gctggttccg ctctccgact cttggtacgt cagccagctg cagacgatgg tggccacact
     1441 gaaaatcccc atggaacggc gtaataaacg gacaggcagg agagagaaag cgcggatctg
     1501 ggaagtgacg gacagaacgg tcagggcctg gattggggaa gcggttaccg ctgacggtgt
     1561 gacgttatct gtcccggtca cgccacatac gttccgccat tcctatgcga tgcacatgct
     1621 gtatgtcggt ataccgctga aggtcctgca gagcctgatg gggcataagt ccatcagttc
     1681 aacggaggtc tacacgaagg tatttgcact ggatgtggct gcacggcacc gggtgcagtt
     1741 ttcgatgctt gagtccgatg cggtgtctat gctcaaaaga attccttaag ctctgacttt
     1801 tttaaatccc tttcggatat ctcatggata tattatcggt atttcatatt ctgttgacat
     1861 gccgggccgg tatagagtaa aaaaaacatc tattgagtac ctcttggata tccgaaaggt
     1921 gtctttgggg ggctttatgg gtattacatg tggattctgg agatacacta tgcctgttat
     1981 tgctaatacg catcccaaag gtggtgtggg taagacaact tcatccgtaa acattgttgg
     2041 tgaaatgaag tctgatacag ttgatctgga tactcatacc gggctttcta tcattctggg
     2101 gctgagacct gaaggaaaag aaatttccgt gaaagtaccc aaaacagtgg atgagttaat
     2161 cgaaattatg actccctaca agaacagcga taagacgtta cttattgact gtgggggatt
     2221 tgactcagat cttacccgta ctgcgattgc ctttgcagac tgcgttattg tcccttcaaa
     2281 agactccctg actgagcgta ttggtttgat gcattttgat ggagtactgg acgaaatcag
     2341 ctctatcatg gggactgata taactgctca cctgtatctc tgtaaagtta atcctaacaa
     2401 gaagaaattc cctaagcttg atgcaatcct gccatcgttc aaacatctga agctgatgaa
     2461 gagccgcatt tctgctcgtg cagaatttga ggatgtcatt gagactggga tgggaatcac
     2521 tgagtctgtt catgggcgct attcggcagg aggtaaggaa gttattgccc tgatcgaaga
     2581 aatcaatcat ctgattgaaa ataacaaaca gtaaagtgta tctattgggt atccatatag
     2641 tattctttgg gtttttaaag gatatctttt gagtatctaa caaacatcct gggttatctc
     2701 atgagcaaag cgaaatttag tgcacaggct gaaaaactga aaaacctggt ggattctgcc
     2761 agattacaaa cttcttcttc tgccgtaaca gcaacagtcc cgtctcagcc aaatgaaggt
     2821 acagaggaag tttcaggtcg tcccgtggcc gccaggacga aggctaagaa tcttaaggcc
     2881 ataccgctgt cgtattttga agctcatgcg gaacttaaag cgaccagtaa aacatctctg
     2941 gattttagct catatattat tgaggctata agagagaagc ttgaacgtga tggcgcaata
     3001 ggtcaataag ctatcggata ccttaagggt atctaatgtg tgtcttttaa atgcgttatg
     3061 gggattctta gtgtacctaa agagtcccca ttagacatct ttctgtatat ggtgacatga
     3121 ttcgtcaccg ctttcctttc ctctcagttt caccatttca ttcccttccc ttaacctgaa
     3181 ccatgatgtc aaagatattt ttatattgca tttatgtatc acatataaca ctcataatgt
     3241 tttttgtatc tatgattttc tctggtctta aggttgtacg tgcgatatac atgcagttta
     3301 ttgtgttttt atgttgcttg taccctttca gaaagaccat ctttccatta taattgaata
     3361 tacattgcat gtatgaagca tatttgtttt ttctatttgt ttatgagcaa cggagactgg
     3421 ttatgaaaat ctttattgat gatggttcta ccaacattaa acttgcctgg ttggaggatg
     3481 gtgacgtgaa aacgttaatc agccctaaca gctttaaacc ggaatggtct tttagtttac
     3541 tggatgatgc tgccccggct aattacgaga tcgacggcga gaagttttca tttgatccat
     3601 taagtgctga tgctgtcgta acgactgaaa cacgctatca gtacagtgat gtgaatgttg
     3661 tggccattca gcatgcgcta cagcagaccg gcctgaaagc gcagcctatt gatgtcatcg
     3721 tcacattgcc gatctctgaa taccttgacg ccaacaatca gaaaaataag caaaacattg
     3781 aaagaaagaa aaaaaatgtg atgcgtgaag tacgggtgca ggggagtgat gcatttgtta
     3841 ttcgcagtgt ttccgttctt cctgaaagta ttcccgctgg tttcagtgtt cttgcggggc
     3901 ttgaggatga tgaatccctt ttgatcgtag atctcggtgg tacaactctt gatgtgtcgc
     3961 atgttcgtag caaaatgaca gggattacaa aaacctggtg tgatccgaat atcggcgtgt
     4021 ccctgattac atccggggtt aaggaacaga tggctgtaca tgctaataca agagtgagct
     4081 cattccaggc tgacaatatc attgtgcacc gtaatgaacc tgactacctt tcccgtcgca
     4141 tttataatgc tgaacagcgt gagtctatca ttaatgttat caatgaacgg cagaagttgc
     4201 ttattaaacg agttaatgat gtcatcagcc gtttcaccga ctatacacat gtaatgtgtg
     4261 ttggtggtgg ggctgaaatt gtggccgagg ctgtgaagaa cctgactaag gttcctgatg
     4321 agcgctttta tctgagctca tccccgcagt ttgatttagt catgggaatg ataaaaatga
     4381 aaggtggtgt gactaatgag tgatgaaaat aaatccagac gctgctcatt tgaacttttc
     4441 ccggatgaac ggacaggaga taagatagca gatgagctca ttgcaaatga aaaactgaag
     4501 gagcggggac gtttcatgcg cgcgatgtta gttactggtg cggcgtttgc agctatagat
     4561 aaacgtcttc ctttgctgat atcggagtta ctgacagaaa atacgactct ggatgatatt
     4621 aataaggtga tttccagcgt aataccaggt gctttttcgg ttgaaaagaa attgcttgaa
     4681 ttactggaaa aacaatctgg tctccatacg tctgtggatt gcagtactcc tcttacagag
     4741 cagagtctgt cgagaaatga cggtgaagac cagaccagaa gaaacgctga aaatatgttc
     4801 ggagatgact gaactaaccc agcctggcca ggggaatatc tcgccattta gttgtataac
     4861 actgactcaa catttcccgt ttcatttgcc attcaggggc ggttcctcgc cccgcaaacc
     4921 acactttccc ttttcccgac tggttcagtt catcgagtgt tttcattaac ttttcgctgt
     4981 ttttatgggg ctggatttca tcaaataatc ccggctgcgc tatacccgat ggtgtgaaat
     5041 cagccagcat gactcctgcc ttcatatagc ggtacccttc acgccagaca tggtttaacg
     5101 ctctgcatgc tgcggcaata atgtcccggc tgtcctgtgt aggcaatgga agcttttcca
     5161 cagcggaatt gctgtaacag gggctttcac tgcaaagggt gacgttcgta taaatgtcgc
     5221 cacctgccgg caatactgac gctccccacg tagtttctct gcggcccgct ctgcatactg
     5281 aacaacagcc tggtgcatgg catctttgtc tgtgattcgt tcaccaaaac tgcggctaca
     5341 gacaatctgc tgttttgccg gtggggcttc ttccggagat atgcaggact cgccgttgag
     5401 ttcgcgtact gtatgctcaa gaatgacgct gaagtttttc cggatgaatg ccgtgttagc
     5461 ctgcgccagc tgctgcgctg tgttaatacc ctgcgcattc agcttttccg tcagtctgcg
     5521 tcctactccc cagacttcac caacaggctg ctgccccagt aacttgaagg tccgattacg
     5581 gttttctgcc gtcagcgcga ccacaccgga aaactgtggc cattgctttg ttgcccactg
     5641 tgcacttttg gccagcgttt ttgtaggtgc aatgcccacc cccatggcga gtcctgtcca
     5701 gctctttact tgttccctga gctgatgacc aaaaacctcc ggagagatgc aacggttcac
     5761 cccccgcaaa tcaatgaaca acgtgagtga caacaccaaa aatacagatg ttttccggat
     5821 acagtgtgcg gaactccggg ctgtctgaaa ccggctccag tgccgggcgt gggcgcaaca
     5881 gcagtcgttt gacggtgaac tccccgtcga tctcagcgat aacgatgtcc ccgtgttgtg
     5941 gtttttcggc cctgtccact accagcagat caccattctg cacgccagcc tggttcatcg
     6001 attcaccgct ggcgcgcaga aagaaggtgg ctgcaggtct gctgatgcaa tagctgttca
     6061 gatccagttc ctgctcagca taatcagtgg caggcgaagg aaaaccggcc tggcaacgat
     6121 cggcaaacaa cggtcgcacg taactgtcat tgcctgaagg gtcagccgga cggtgataaa
     6181 cggtactcat gctgcaggca gccagctgtc gtcctgccag atttcttcca gcagagacag
     6241 gatacgttct ttatccttgt cgttgcgggc tcctgtgaca gatacagacg gggcgctgcc
     6301 ctggtctata cgcagccaga tttccgggta gtgtggtttc agacgtcgtt cgatttcatt
     6361 ctgtagcgcc tgaagtgtgc ctgatttgag gttttttgtg ctctggcggt cgaaaagaat
     6421 ttcaatgcgg atcatgttct catctccctt ttcttttgct gtatgcatat acagtattct
     6481 aataactgaa tccacacaca gtcaaatgtc attattgtga ctaaaaaacg ctcaattctc
     6541 tgagggtgtg gctgtatgcc tgctgtggac ggctgtgtcc agcttatcca cagcattttg
     6601 tgcacggttc tgtggacaaa atacctggtt acccaggccg tgccggcacg ttaaccgggc
     6661 tgcatccgat gcaagtgtgt cgctgtcgcc ggcctcctca cccggtcacg tttcgtcgtt
     6721 cctcctccac gcgctgcggc ttcggggccg cacctgcatt cgtatgcggt cgcccggtta
     6781 caggtgcggc acggcctggt ggaggccgca tgtgagagga gaattcccat gccaaactgg
     6841 tgctcaaatg tgcgacatga agtcgcctga ataattgttc cagcggagtt cgattccgtc
     6901 agggaacctg atgttccgtc atcagtagcc tactgacaca tgcgtcactg gtaacggtgg
     6961 ggtgtgaagc tgtcaggaca atgaaaccgg atcttcggat cgcatgaaac cgtgaggtta
     7021 catgtaatct gccagcatca ggcggaggag gtctaggctc ggtagcatga ctaacatatg
     7081 tgaactgctg aagcgtcgtt aaccagaaca agccaaagat gctgacaggc ttgaaccaaa
     7141 aggtatgtgg ttggttactc ctctgtttgc taggggtaca cggacaataa accaccggtg
     7201 ttgtgagcag agtctaacct actgttgtta ttcaggtgga acatggtaag cccgtatcgc
     7261 tgcctcgtga ggcaggttaa ccgcaaggga tactgttggc ggtgcgggta aaagaaggtg
     7321 gaaaaagcga atgccagtct gtaatggacc ggataagggt tgagcccggc aacattaccc
     7381 tacgcgaaag cgggcagact tccactgggt ctttcattac gagagagttt gagtaatctt
     7441 ccagaaagga aaagcagatg actgagcagg ctacaacctg taaaggtgcg tccttactta
     7501 acggtgactc ctggcacagt atcaactggc gtcagtgcta tcgggaagtg cggagactgc
     7561 aagcgcgtat cgtaaaggca acacgagaag gtaaacatgg caaggtcaaa tccttgcaat
     7621 ggatactgac tcactcgttt agtggcagag cagttgctgt cagacgagtt acagagaata
     7681 gcgggaaacg tactcctggg gttgatggtc agacatggtc ttccccggag gttaaatttc
     7741 ttgctattaa cctgctgaaa cggcgtggtt acaaaccaca gccacttaag cgggtttata
     7801 tacctaagtc aaatgggaag tctcgtcccc ttgggatacc cactatgaag gatcgggcga
     7861 tgcaggcgtt gtatttgctg gctcttgagc ccgttgctga agtcactgct gaccagagat
     7921 ccttcggctt tcggactgga aggtctactg cagatgcgat agcacagtgc ttctgtgttc
     7981 ttgcgcagaa aacatccgct gaatgggtgc ttgaggggga tatacgggga tgctttgaca
     8041 atatatctca tcaatggcta atcgataaca cctcaacaga cagacaaatc ctgacaaagt
     8101 ggcttaaagc gggttacagg gaaaaggggc agttattccc tgtaaattca ggaactcctc
     8161 aggggggaat aatttctcct gtactggcta acattgctct ggatggactg gaggcgcttc
     8221 ttgcttcgga gtttaaaaag agaacagtca agggacgatt ggttaacccg aaagttaact
     8281 atgtgagata tgctgatgat tttattatca ctggagagtc aaaagaactt cttgagtctc
     8341 aggtattacc cgtcgtcagg cggtttatgg ctgaaagagg attaatgctt tctccggaaa
     8401 agacgaaaat cacacatatc gaggaaggat ttgattttct tggacagaac attcgcaaat
     8461 atggcggtaa gatgctgatc aaaccctcta aagccaatgt cagttccttt ttaaagaaaa
     8521 tacgcgctgt aatcaaaggc aacaaagcta tggatcagct tacgttgatc cgcatgctga
     8581 acccaatgat caaagggtgg gcggcttacc atcaacacat tgtggccaag gtcgcattta
     8641 acaaagttga caatgagatc tggctggctt tatggcgttg ggctgttcgg cgtcatccca
     8701 ataagggaaa gaagtggatt cggaaaagat atttccatca acaaggtgcc cgaaactggt
     8761 ctttctccac ggctaccgga gaactccttg ccaacggtaa gcctcagtat gcaaatctga
     8821 ggaaagcaat cgatacgcct atcaaccggt ttaaaccgat taaaattgca gctaatccgt
     8881 tcgatcctca atgggagatg tactttgagg agcgttgtgc agacaaaatg cggcacaaac
     8941 tgcaaggacg gaaaaagctc attcaaatct ggtttgagca gcggggccga tgccccatat
     9001 gtgatgagag gattacatct gattcccagt ggcaggttca tcacatcata cggcgagtcg
     9061 atggagggag caactgcctc tccaatttga ttatgctgca tccgatgtgt cacacacttg
     9121 ttcatgcgaa aggtatacat gtagtgaaac cggctcatga gagtgggctt agaaaggctt
     9181 gagccgtatg ccgggaaact ggcacgtacg gttcttaggg ggcggtgatg cagtaatgca
     9241 tcactgctac ccgatcgtat gcatttttct ggtgaaccgg cacagattgc tgagattaaa
     9301 cgactggcca gcggtgcagt cacaccgttt tatcgccgcg ccacaaatga aggtattcag
     9361 ctgtttctgg ccggaagtgc cggacttctg cagatcactg aaaatatccg ctctgagcag
     9421 tgccccggag tgacggctgc cggacgtggt gctgtatcga cggaaaatat cgcgttcacc
     9481 cgctggctga cacacctgca gaacggtgta ctgctggatg agcaaaactg cctgatgctg
     9541 catgaactct ggctgcagag cggtactgga cagcgtcgct gggaagggtt accggatgat
     9601 gtcagggaaa ccatcactgt gcatttcacc gcaaaaagag gtgactggtg tgacatctgg
     9661 ggtaacgagg atgtatcggt gtggtggaac cgtctgtgtg acaacgtagt gccggaaaaa
     9721 accatgccgt ttgacctgct gacggttctg ccgacccgcc tggatgttga agtgaatggc
     9781 tttaacggtg gtgttctgaa cggtgttcct tctgcatatc actggtatac agaacggtat
     9841 ggcgtgaagt ggccttgtgg gtatgacctg aatatcagca gtcaggggga aaacttcatt
     9901 caggtggatt tcgacacgcc gtggtgtcag ccggaaagcg acgttgttgc agaattaagc
     9961 cgccgtttcg gctgcacgct ggagcactgg tacgccgaac agggctgcaa tttctgtggc
    10021 tggcagcgat atgagcgtgg agagcttgtt gatgtgctgt ggggggaact ggaatggtct
    10081 tccccgacag atgacgatga gctgccggaa gtcaccggac ctgcgtggat agtcgacaag
    10141 gtggcgcatt acggtggatg aagtatgacg gagacgggcg ggcaactgcc cgtttctttt
    10201 ccggtaaagg atgtcgccat gctcctcttt ttactgcgcc ggctgacgcg gcgcggcata
    10261 aacgctgcct gtggtcagtg tcccgcgtcc ggcgggcacg ggacgggcgc ttttaccgct
    10321 cccggctggc gtcggtgaca gtgtacgcca gcctgtcgcc cttttctgat gaacgccctt
    10381 caagccgctt tcgcggcata accttgccgt caaacagacg acggctgcgg tattccacgg
    10441 tcggcctgac ccgttaccgg acgcggtgaa cagcccacag gcagcgggga acgggcacca
    10501 caggggtgcc ctcccggtgc cctctgtaag agaaggcgtt ttgtatgtcc cgttttgtcc
    10561 tcggtaactg catcgatgtt atagcccgta tccctgataa cgccattgat ttcatcctca
    10621 ccgacccgcc gtacctcgtc ggtttccgtg accgttccgg gcgcaccatc gctggcgata
    10681 aaaccgatga atggctgcaa ccggcctgta atgaaatgta ccgcgtactc aaaaaagatg
    10741 cgctgatggt cagcttctat ggctggaacc gcgtcgatcg ctttgtggcc gcctggaaaa
    10801 atgcgggatt cagcgtggtt ggtcacctgg tcttcaccaa aaactacaca tcgaaggccg
    10861 catatgtggg ctatcgccac gaatgcgcct acatcctggc aaaaggccgt ccacgtctgc
    10921 cacaaaaccc gctgccggac gtgctgggct ggaaatattc gggcaatcgc catcacccga
    10981 cggaaaagcc cgttaccagc ctgcaaccgc tgattgagag cttcacacac ccgaacgcaa
    11041 ttgtgctgga cccgtttgca ggcagcggct caacctgcgt cgccgccctc cagtccggac
    11101 gccggtatat cggtatcgag ctgcttgagc agtatcaccg tgccgggcag caacgccttt
    11161 ccgccgtgca acgggccatg cagcaggggg ccgcgaatga tgactggttt atgccggagg
    11221 ctgcgtaaat gcactatgcc ggacacgaaa aacttcgcgc cgaagtggcg gaggtggcca
    11281 atgccatgtg cgacctgcgt acaaccatga atgagatgga gcggcggtac agctttaatg
    11341 ctgacaccct tccggaacgt ctggtgcgtc agacgttgtt tcgcgcaaac cgcctcctga
    11401 tggaggcata taccgaaatt ctggaactgg atgcgtgctt taaagattga gaaggagacg
    11461 agaatgtacg gaacatgcga aacgctatgc cgggagctgg cagcaaagta tccgggagac
    11521 acgccgttga tgctggttgt ctggtccccg gaagagattc aggccctcgc tgacggaatg
    11581 gatatttccc tgtccgatca tgaaatcagg accgtcctgg cgcgcctgga ggacatcccg
    11641 gaagaccagc gaattgagtc cggtatttct tccgccgcgg cgatggagat tatcagcaac
    11701 gtgagcgaaa accgccaggt gaccgtccct gctgaactgc tggcgtccct gattcagacc
    11761 gctgaacagg cattgtggaa gcgtgaatgg gccgcccgtg attacggcct cgccgtcccg
    11821 gaatgcgtta cccgccgtca ggcggtggtt aatcaggccc gcatcctgct gaaaaacaac
    11881 acgcacgaaa acgactgatg ttatcgccgc ctccgggcgg cgattcaggg agaccatgaa
    11941 tcatgaataa aacgctgaat gcactgattt gtcgtcatgc ccgcgacctg ctgctggcgc
    12001 agggctggcc ggaagagacg gatgttgacc agcgtaacct gaagtatccg ggctggatca
    12061 gcatttacgt gcggctggat gcgccccggc tggcgacgtt actcgttaac cgccacgatg
    12121 gcgtactgcc gccgcacctg gcctccgcca ttcagaaact gaccggaacc ggggcggaac
    12181 ttgttctgtc cggcagtcag tggcagtcgc tgccggtact tccggcagac ggaacacagg
    12241 tgtcttttcc gtatgccggt gaatggctga ccgaagacga aatcagggca gttcttgatg
    12301 cggtgcgcaa tgcagtacga agcatctgtt accaggtggc agacgatgcg cggcgtatcc
    12361 gggcggcgct gaccaccacc ggtcagacgt tgctgacccg ccagacgcgc cgctttcgtc
    12421 tggttgtgaa ggaaagcgat tacccctgct ggcttgatga agatgacgaa aatctgcccg
    12481 tggtgctcga tgccattctg aaccgtggcg cacgtttttc ctctgtggaa atgtacctgg
    12541 tctgtgagtg tgtggagcat atcctggcca gtggtctggt ctgtgatgtg ctgcgtatac
    12601 cggatgaacc gtcccgtcgc tggtttgacc gggatattct gcgggaggtt gttctggagg
    12661 cacgggatga aatccgcagc atggcggatg ccctggcaaa aatcaggggc tgatgatggc
    12721 ggggaaatcc ccgccttttt cctgccggtc cccggacaga gcacaaagga agtcgccggc
    12781 ggctccgctt tacccagcca tgcggggcat ggccttgtgg attttcagtt atgtggccgc
    12841 cgtgtcgtgt gcgggctgtg ccgtgcctcc atcttagccg ggctggcagg gatgcaaggg
    12901 tacgcttcgc cgctgcggtc acccggtccc tccttcccgt ctgccgtgat tttccggtcg
    12961 ttcacccgct cagatttcgc tgtctcaccc tccaactccc gtcagccgtg tgcgtcaggc
    13021 tgcggcttcc cttgcgccct gtgcatcccc gccttatcgc ccggctttta tggaggcacg
    13081 gcaccgcccg gtgtcgcgga atatgtgaac aacggaggaa aacatcatgc agtatgcgaa
    13141 acctgtcact ctgaacgttg aagagtgcga ccgtctgtct tttctgcctt acctgtttgg
    13201 cctggatttt ctgtatgccg aagcgtctgt atacgcgctg gcgaaaaaaa tgatgccgga
    13261 atatgaaggc ggattctggc acttcatccg cctgccggac ggtggcggtt acatgatgcc
    13321 ggatggcgac cgttttcatc tggtcaacgg tgaaaactgg tttgaccgca cggtcagcgc
    13381 cgatgccgca ggcatcattc tgacttccct tgtgattaac cgccagttgt ggctgtacca
    13441 cgacagtggg gatgcaggac tgacccagct ttaccggatg cgcgatgcgc agttgtggag
    13501 ccacatcgaa tttcaccccg aatgtaacgc gatttacgcg gcactggact gattaacgga
    13561 cggggcggca atgccgcccc tgtaaagaag acgagaacat gtactgttaa agaaattatc
    13621 cgcgatgtac tggatacaga cgtgccggac agtgaatgcg ttttggccgt ggtgctgacc
    13681 cgtggggatg tgcgccatat ggctcaggac tggaacctgt cagacgatga gctggaaacc
    13741 gtcatgcagc ggctggacga tgcctttgaa tatggtgcgg atgtcagcat tgttcatgac
    13801 gttgttcgtg aactgatgga agaaaagcgc gccagccgtc aggtgacagt tccggcggtg
    13861 atgctggaaa aagtgatggc gctggctggc agtgaaatga agcgcctgta tgccgtcggg
    13921 agtgagaacg ggggcgacgg tgacgcattc gtcagggaag aacgcgaagc aatggacgtt
    13981 gtgttacagg ggcaggcggt ggaggccgtc tgtcagcccg cgcgaccccc cctggcaggc
    14041 tgacgcctgc cggaagggaa gcgggccacg ctgacgcgtc gcccgttacc cttcccgttt
    14101 gtgatgtgtc cggaatgttt ttttctgatg ttatttttct gtggttaccg tgcttgttat
    14161 tgttcttctt cctcttcttc ttttcgttgc tctcctgttt atggctttcc cgggcgtccc
    14221 gcttgcgggg cgggctgtcg tgaacggagc cagcggagct gtctccgccc tggcgggctt
    14281 ccatccctga cgccgcaaaa ccccgggctt gcagcgaccg gaacggtcgc gccacccttg
    14341 tcgcgccgtg gcctgtgctc ctggcatccc ggcaaaaggc cgccgggatg tgaggcgcaa
    14401 cgccgtttcg cgcacgcgct ctccgtcatt gcggcgggtc cctgctcatt gcctgccgga
    14461 tgccacacac ccgtatgagt ccctgtcgcc gggggctcat acagccacaa cctgccgcac
    14521 tgactgtcca gggtaaatga acagcccaca aaatttttta cccaaaaccc ggtaaaaaat
    14581 tttgcgttct gcccaggaca ggtgcgtcag gccgtggcag tgatgcccct tgcggcgaag
    14641 gttctcaaac gggtgaagtt cataccacag ggaatacagc agcaggttcc cgcgaaacgt
    14701 caaaaccgcg gctcccgaaa ctcaacgggt gatgttcttt aaaaatcagg atttatttca
    14761 ggctccggca atacgcattt atgtgttctg tcagtcatcc ggctggcagg taatgagaac
    14821 gcaggaacag aaggaggaaa acaccggaga acgtcagacg cagatatgaa aaaagcaggt
    14881 cagggacctg cttaatccac accgcagaga ctgcacgaac agtctttacg gtttcattaa
    14941 ttaacgtcat aagaggctaa attatgacag taagtattgt ttcaccttcc gcagcggctg
    15001 taaagccccg ccgtcatccc cgtttcagac gcgaagacat ccccgcgccg gaaattgacc
    15061 ccgtgctgaa agccttcggg cgacacattg cccggagttt tcaccggggg cgtggtgtac
    15121 acatccccgc catgaaaaac acggccttcg ggcaggtgct ccgcacactc gaactgaaac
    15181 gcgcttttaa ctgatgaggt gagccgcccc gcgaggggcg gcaacagacg gaaggagaac
    15241 attttatgac atcagcagca atcaccatga ccgccccgga agccgccagc cctgtgcaga
    15301 tgtaccgcgc gacctactca ccggatgaca acaaactgcg cctgtatgcc gtgtcacgtc
    15361 ttgacccgga gacgtataaa aaagtgcatg atgccggttt ccgctgggca ccaaaacagg
    15421 cgctgtttgt cgcgccagcc tggacaccgg gccgggaaga cgtgctcctc tcacttgccg
    15481 gagagattga ggatgaagac agcacgctcg ctgaacgtca ggaagcacgg gcggagcggt
    15541 ttaccggata cagcggaaag cgggccagtg aatccgcaca ggcacttgat gaagtggaaa
    15601 gactggccgc gatgatcccg cccggtcagc ccattcttgt ggggcatcac agcgaacgcc
    15661 gcgcccgtcg tgatgcgcag cgtattgaaa acggcatgaa acgagccgtg atgctctttg
    15721 aacgtgcgga atactgggaa gagcgggcgc ggtcggcact gcttcacgcg aagtataaag
    15781 aacgtccgga cgttcgctgg cgtcgtatca aaaaaatcga agctgatttg cgcaaggctg
    15841 aaaagaccat cgcgcagtcg cagaaatatc tgacgatgtg gcgggctgaa tcgctggatc
    15901 tgaatatggc aaaactcatc agcagtcatg accatatcag cgcctgtttc ccgctggata
    15961 cgtatccgcg cccggcagaa aaaagccagt atgaaggaag tcgatcgtta tggtcggccc
    16021 tggatgatga catcatcacc acggagcagg cccgcgaaat tgcgatccgc tgtcatgaac
    16081 ggcagattca gcatcaacaa cgctgggtta accactatca gaaccgcctg atctatgagc
    16141 gtgccatgct ggacgaaagc ggcggcgtgg ttacccggac acaggatttt gagccgggcg
    16201 gacaggtttt cagccggggc gagtggctga ccatcatccg tgtgaacaaa agcaacgggg
    16261 cggtgagttc agtcacaacg ccaaattaca gttttctcgg gtacagcggc acgatgaaag
    16321 tgacgcccga tcgcatcacg gactacaaag caccatcggc agaagaggct gccgtcgcca
    16381 gtcaggccgc gaagcgtccg ccggtagtca actatccggg ggaaggtttc cgggaaatga
    16441 caaaggcaca gtgggccgcc ctgccccggg actgtaaggc cgtgcgcagt gtggcagaag
    16501 cagaagacca cggggcatac cggtaccgcc gcacaatgga caataatttc cgcctggtga
    16561 atgtgtatat caccgacatg aaaatcacgg aaatcccaca gaaataaggt atatcccccc
    16621 gggaaaatcc ccgggggatg acggagataa tgatatgcac agtcagttaa gagaacgtat
    16681 ccggctgatg cgcgcaaggc tggataacgc cgcgccggtt gctgaaatcc gggctgaatc
    16741 tcagcttttt gtgactcctg ccccggtctg tgatcgcctg gtgatgctgg cggagatcag
    16801 caaccgtgat cacattctgg aaccctctgc cggcaccggg gccatactgc gggcgattcg
    16861 ggataccgcg ccgggggcca tgtgtgatgc ggtggaaatc aacagcgggc tggtccggta
    16921 tctgcgggaa aattttaacg gtgtcagggt tcagtgtggc gacttcatgg aatggcagcc
    16981 agtgcagtat tacagccggg ttatcatgaa tccgccgttc agccacgggc aggatatccg
    17041 gcatatcctg cgggcctttt ccctgttgcg tccgggtggc gtgctggtcg ccgtctgtct
    17101 caacggacca cgccagcagg agaagctgtt accgttttct gacgtccgcg aggagctgcc
    17161 gcgcggcacg tttgcttata ccgatgtccc gacgatgatt attcgtctgc gtgcctgatg
    17221 acacagggcc ggcgggagga taaatgcctg tctgcccgcg ccggtggcgc tgaaaggggc
    17281 cgcgctcacg cgccgcccct ttcattacgc ggacgttctg atgggggctt ctgtttcttc
    17341 atgcctgtca tttgtctgtt actgatgatg ttgttgttcc tgctactgct actgctactg
    17401 ctactgctac tgctactgct actgctactg ctactgttct tctttttcct gctgctctga
    17461 ttctgactac tgcagtgttg tcctgtctgc cgggcgcacc ttcggctccc ggctgcctgg
    17521 cggcatccgg tcgtcaggcc cgactccgac aggcagctgc tggcgcacct gccagtctcc
    17581 gccgaacccg ctggcgcggt ttcgggctgt cgctacggtc cgatgcgtga aaaccccggg
    17641 cttgcagcga ccgttccggt cgcgccgcct ctgtcgcgcc gtggcctgcg ctcctggcat
    17701 cccggcaaaa ggccgccggg atgtaagggg caacgccgtt tcgcaaaccg cgcttcacgt
    17761 cgttgcggcg ggtccctgcc cgttgttctg tccggtgcca ttcacctgtt taagcccctg
    17821 tcgccggggg caaagacagc cacaaccgtc tccgccagtc caggggtgaa atgtacatcc
    17881 cgcaaaactt tttgcccaaa agcaggcaaa aacttttgcg gtctgccccc ggacagtcgt
    17941 ttccgtccgt ggcagtgatg ccccttgcgg cgaaggttct caaaaaggtg atggtgaata
    18001 ccggagggaa caaaaggcag gtccccgcaa aacgtcaaaa ccgcggctcc cgaaactcaa
    18061 aagatgatgg gggctgaaaa catcaacaga aggaggcaca tcatggcagt tcgtggcatt
    18121 aacaaggtca ttctcgtagg acgcctggga aaagatccgg aagtccgtta catccccaac
    18181 gggggcgcag tggcaaacct gcaggtcgcc acgtcggaaa cctggcgtga caaacagaca
    18241 ggggagatgc gggagcagac agaatggcat cgcgtggtgc tgttcggcaa gctcgcggaa
    18301 gtggctggcg aatatctgcg caagggcgcg caggtctaca tcgaaggtca gcttcgcacc
    18361 cgtagctggg aagataacgg tatcacccgt tacgtcaccg aaattcttgt taagaccacg
    18421 ggcaccatgc agatgctggg acgtgccgca ggtgctcaga ctcagccgga agaggggcaa
    18481 cagttcagcg ctcagcctca gccggaacca cagtcggagg ccggtacgaa aaaaggtggc
    18541 gcaaaaacga aaggccgtgg acgtaaggcc gcgcagccgg agcctcagct gcaaccgccg
    18601 gagggtgacg attacgggtt ttcagacgat atcccgttct gatcgggctg actgtgataa
    18661 ccgccccgcc ccgttctgtg cggggcatca ccggagagat gagaatgagc gaatatttca
    18721 gaatacttca ggggctgcct gacggtcctt ttacccgcaa acatgccgaa gctgttgccg
    18781 cacagtaccg gaacgtcttt atcgaggatg accacggcga acagtttcgc ctggttgtcc
    18841 gtaataacgg tgcaatggtc tggcgtacct ggaattttga ggacggtgcc gggtactgga
    18901 tgaaccatgt catcagggat ttcgggattc ttaagtaaga gaggtgccgg acggggacac
    18961 gtccggcaga caatcattat taccgttttg ggaggtacta actatgtcag ttacagagtc
    19021 taaggcaaaa acggagcgta aatccagccg taaacctgca aaaacgcagg aaacagtcct
    19081 gtctgccctg ctggcgcaga cggaggaagt gagcgtgccg ctggcctcac tgattaaatc
    19141 accgctgaat gtgcgcacgg tgccgtattc tgcggagtcc gtcagcgaac tggcggactc
    19201 cattaaaggt gtcggcctgt tacagaatct ggttgttcat gccctgccag gtgaccgcca
    19261 cggtgtcgcc gcaggtggtc gccgactggc agcactcaac atgctggcag agcgcaacat
    19321 ccttccggcg gactggcctg tccgtgtgaa ggtcattccg caggaactgg cgactgccgc
    19381 atcaatgact gaaaacggtc atcgtcggga tatgcaccct gccgaacaga ttgccggatt
    19441 ccgcgcaatg gcgcaggaag gcaaaacacc tgcacaaatc ggtgatttgc tgggctattc
    19501 accccgccac gttcagcgaa tgctgaaact ggctgacctt gcgcctgtca tcctcgatgc
    19561 gctggcagaa gaccgcatca ccacagagca ctgtcaggcg ctggcgctgg agaacgacac
    19621 cgcgcgtcag gtgcaggtgt ttgaagctgc ctgtcagtcg ggatggggcg gaaaaccgga
    19681 agtacagacc attcgtcgtc tggtgaccga aagtgaagtg gcggtggcgg ggaacactaa
    19741 attccgcttc gtgggggctg atgccttctc gccagatgaa ctgcgcactg atttgttcag
    19801 cgacgatgag ggtggctatg tggactgtgt tgcgctcgat gccgccctgc tggaaaaact
    19861 ccaggctgtt gcagagtacc ttcgggaagc cgagggctgg ggatggtgtg ccggacgcat
    19921 ggaggccgtt ggtgagtgcc gtgaagatgc ccgggcatac cgcaacctgc cgcagccgga
    19981 agcagtgctg acggaggcgg aagaagaacg cctgaacgaa ctgatggcac gttacgacgc
    20041 actggaaaac cagtgtgagg aatccgacct gctggaagca gaaatgaagc tgatgcgctg
    20101 catggcgaag gtcagagcgt ggacgccgga gatgcgtgcc ggaagcggtg tggtggtgtc
    20161 ctggcgttat ggcaacgtgt gtgtccagcg tggtgtgcag ttgcgcagtg aggatgacgt
    20221 ggctgacgac gctgaccaca cggaacagat gcaggagaaa gcgtcagtgg aggaaatcag
    20281 tctgccactg ctgacgaaaa tgtcttccga acgcacgctg gcagtccagg cggcactcat
    20341 gcagcaaccg gacaaatctc tggcactgct ggcatggacg ctctgcctga atgtgtttgg
    20401 cagcggagcg tacagtaaac cagcacaaat cagcctggaa tgtaaacatt attcgctgac
    20461 cagcgatgcg ccgtcaggga aggaaggtgc cgcattcatg gcgctgatgg cagaaaaagc
    20521 ccggcttgca gccctgctgc cggagggatg gacacgggac atgacgatgt tcctgtcact
    20581 cagcctggag gtgctgttat ccctgctcag tttctgtacc gcgtgcagtc ttaacggtgt
    20641 ccagacccgc gagtgtggtc acacgtcacg cagtccgctt gactcgctgg aaaccgctat
    20701 cggattccac atgcgcgact ggtggcagcc gacaaaagca aacttcttcg gacacctgaa
    20761 aaagccgcag attatcgcag ccctgaatga ggcaggactg tccggtgctg cacgggatgc
    20821 agagaagatg aagaaaggtg atgcggctga acatgcagag caccatatga aagacaaccg
    20881 ctgggtgcct ggctggatgt gtgcaccaca tccacagacg gataccactg aacgcaccga
    20941 taacctggct gatgccgcct gataaacaac cacaccgccc cgccggagac ggggcggcag
    21001 caagggagat accgtgatga aaactgaact gaccctgaat gtattacaga ccatgagcgc
    21061 acaggaatat gaagatatcc gggcagccgg aagtgatgaa cgtcgtgagc tgacacacgc
    21121 cgtgatgcgg gaactggatg cgccggacaa ctggacgatg aacggcgagt acggcagcga
    21181 gtttggcgga tttttccccg tccaggtccg tttcacgcct gcccacgaac gtttccacct
    21241 ggcgttatgt tcaccgggcg atgtctctca ggtctgggtg ctggttctgg tgaatgccgg
    21301 tggtgagccg tttgcggtgg ttcaggtaca gcggcggttt gcaccggaag ccgtcagcca
    21361 ttcactggca ctggcggcgt cactggacgc gcaggggtac agcgtcagtg acatcatcca
    21421 tatcctgatg gcggaaggag gtcaggcatg agcgcacgtt cacaggcact ggttcctctc
    21481 agcacagagc aacaggccgc atggcgggcg gtggcggaga ccgaaaaacg ccgccatcag
    21541 ggtaacacgc tggctgaata tccgtatgcg ggggcgtttt tccgctgtct gaacggaagc
    21601 cgcaggatat cgctgtctga cctgcgattt ttcatgccgt ccctgaccgc agaagaactg
    21661 cacggcaacc gtcttcagtg gctgtatgcc atcgatgtgc tgattgaaac acagggcgag
    21721 gtctgcctcc ttccccttcc cggtgatgca gcagagcgct tgttcccgtc ggtgcggttt
    21781 cgtgtcaggg aacgcagtcg ccataaatcc gcgctggtca tgcagaagta cagccgccag
    21841 caggcgaggg aggcagagca gaaagcccgt gcgtaccagg cgctggtcgc tcaggctgaa
    21901 atcgaactgg cattccattc gccggaaaca gtcggaagct ggcatgcacg ctggagtgac
    21961 cgggtggcag agcatgacct ggaaaccctg ttctggcagt ggggcgagcg ttttccgtca
    22021 ctggccggaa tggaacgctg gcagtggcag gatatgccat tctggcaggt catcgcagag
    22081 gctggtatgg cggcaagggt ggtcggtcat gctgttcgtg agatggagcg gtggatggtg
    22141 ccgaacaaac tccgggaggc agcgtgatgc ggcaacaatc acacggattt cccgtgaacg
    22201 gtctgaatga gtggattatt ttcagggaaa gtgagtgtgg tcagcgtgca ggtatatggg
    22261 ctatgatgtg cccggcgctt gaggctttct gcctcatgac gtgaaggtgg tttgttgccg
    22321 tgttgtgtgg cagaaagaag atagccccgt agtaagttaa ttttcattaa ccaccacgag
    22381 gcatccctat gtctagtcca catcaggata gcctcttacc gcgctttgcg caaggagaag
    22441 aaggccatga aactaccacg aagttccctt gtctggtgtg tgttgatcgt gtgtctcaca
    22501 ctgttgatat tcacttatct gacacgaaaa tcgctgtgcg agattcgtta cagagacgga
    22561 cacagggagg tggcggcttt catggcttac gaatccggta agtagcaacc tggaggcggg
    22621 cgcaggcccg ccttttcagg actgatgctg gtctgactac tgaagcgcct ttataaaggg
    22681 gctgctggtt cgccggtagc ccctttctcc ttgctgatgt tgtacgggca tgaacaacct
    22741 gacttcagga aggtcgtttc ccttcaggaa cggggatgaa cgtgcgcctg cggcgcccgt
    22801 tcttttcccc cgccttctct ggttatggcc tgtcagaaat cacatcacct tccctgtgat
    22861 tattctcttt ttcttcctgc tctgattctg actactgcag tgttgtcctg tctgccgggc
    22921 gcaccttcgg ctcccggctg cctggcggca tccggtcgtc aggcccgact ccgacaggca
    22981 gctgctggcg cacctgtcag tctccgccaa acccgctggc gcggtttcgg gctgtcgcta
    23041 cggtccgatg cgtgaaaacc ccgggcttgc agcgaccgtt ccggtcgcgc cgtggcctgc
    23101 gctccttaca tcccggcaaa agaccgccgg gatgtaaggg gcaactccgt ttcgcaaacc
    23161 gcgcttcacg tcgttgcggc gggtccctgc ccgttgttgt gtccggtgcc attcacctgt
    23221 ttaagcccct gtcgccgggg gcaaagacag ccacaaccgt ctccgccagt ccaggggtga
    23281 aatgcacatc ccgcaaaagt ttttgcccaa aagcaggcaa aaacttttgc ggtctgcccc
    23341 cggacagtcg tttccggccg tggcagtgat gccccttgcg gcgaaggttc tcaaacaggt
    23401 gatggtgaat accggaggga acaaaaggca ggtccccgca aaacgtcaaa accgcggctc
    23461 ccgaaactca acagatgatg ggggctgaaa accagagaga ggagaaagca ggatgagtac
    23521 ccgaaatatc cacgttaaca ccgcgtcgta cacgcttctt gttgccggga agaaaaaaaa
    23581 caccggggaa gagtgggacg tcctggaatt cagcagcctg actgagctga aaaaatatcg
    23641 caaaagccac ccggaaaaga tggccttcag ctatagctac gcgctcagcc ggggtgtgga
    23701 tacgcagttc cgccatatca acattgcgga agccgatcac ttcaaacagt tcctgcgtca
    23761 gattaagcgt gccggcctcg atatccgggc tatctgctga ccggatgtga tgaatacccc
    23821 ccctgattgt tgcaatcttg ttaatggaga tcgggaggga tgatattaag cggaaaaatt
    23881 ttttattaaa tttattcttt cataaaggag tagctgttat gcgattagct tcccgttttg
    23941 gtcggtataa ttccatccgc cgtgaacgtc ctttaacgga tgatgaatta atgcagttcg
    24001 tgccttcggt attttccggt gataaacatg agtcccggag tgaacgttat acgtatattc
    24061 caacaatcaa tatcatcaat aagttacgtg atgaaggttt ccagccattc tttgcctgtc
    24121 agagtcgggt tcgtgatttg ggacgtcgcg aatacagtaa acatatgtta cgtcttcgca
    24181 gggaagggca tattaacggg caggaagttc ctgaaattat cctgcttaat tcacatgatg
    24241 gttcatccag ttatcagatg aaccccggaa tttttcgttt tgtctgcaca aatggcctgg
    24301 tgtgcggaaa taattttggc gaaatccgcg ttccacataa aggtgatatt gtcgggcagg
    24361 ttatcgaggg tgcgtatgaa gtactcggtg tctttgataa ggtcactgat aatatggagg
    24421 caatgaaaga aattcatctt aacagtgacg agcaacattt atttggcaga gctgcactga
    24481 tggtcaggta tgaagatgaa aataaaacgc cagtgacgcc tgaataaata attactcccc
    24541 gtcgtcggga ggataaacag aacgatctct ggacaacctg gcagcgggtt caggagaata
    24601 tgataaaagg tggattatct gggcgaagtg cctccgggaa aaataccagg acaagagtca
    24661 ttacaggtat tgatggtgat ataagaatca acaaggcgtt atgggtgatt gccgaacagt
    24721 tcagaaagtg gaagtcatga taatataagt aaagccccga aaatttttcg gggctttact
    24781 tatattatca ttcttcggca tcgctgtgtc gtctgatata cctcaatttc atcaactggc
    24841 tttccggttg tctgtaaata ttgtgcccgc gaaaaatggt ctggcaggag gctgcgcagt
    24901 taccgggtat atataagatg ggttcccgct tcgcggggct tcggccagtc agtattgtca
    24961 ctcatatgat atttttgtgt gtggccttcc atgccgcttt tgcggcataa caggtatctg
    25021 acaatgtctg taagattaat tgttctgcac gctgttaatt tccggaagtg atttcttcgt
    25081 ggccggaatc cggatacctt tactgctttt tatcccggta taaatccggt aaacatctga
    25141 tgcgtaggca agacgtctct ggttctggcg ttcggtcttc ctgaatccgg cattgtatgc
    25201 accaacggcc tcccaggaga cgccccattt tttaaatgct attgccagat aataagcacc
    25261 ggtataaatg ttcatgcagg gatctgttgt cagatgttct ggcttaattc cgtagcgggc
    25321 cagttcgtta aaatgctggg aatctacctg catcagtccg ctgccatatc ccgttaccgg
    25381 attaataccg atggcattaa cccggtaacg ggattctttc catgatattg ctctcagtaa
    25441 atcaggatct attttgtaat cccggcctgc aagatcaaag caatcagtgg cctggcagat
    25501 ctcatttata aacatcaggc agatggctaa catccacttt ttcatttttc cacctctggt
    25561 gactttatcc gtaaataatt taacccactc cacaaaaagg ctcaacaggt tggtggttct
    25621 caccaccaaa agcaccacac cccacgcaaa aacaagtttt tgctgatttg tgttttaaat
    25681 catgtaatta atgaactata tttatattat ataattttga attatattgc agagattcgt
    25741 gatagattcg atagtgaatc tatcacgaat tgtcaatgat tcagtaatga ctattttttt
    25801 tgaaaacatc tatttacttg gcgcgtgaat ctatattaga ttcgtcattt gatgattgct
    25861 gattcgtata gtgaatcgtg attttagtat gatgaatcta atggtgattc gtcataaggt
    25921 gcagaataga gagcacgaaa atatattgtt tgtttaggaa ggtgaataag gtgtgttatg
    25981 gccaaaattc aggtttatgt taatgaccat gtgtctgaaa aaataaatgc tattgctgtg
    26041 caaaggcgag cagagggagc aaaagagaaa gatgttagtt attcaagtat tgcctctatg
    26101 ttgcttgagt taggattgcg agtttatgaa gcgcagatgg aacgaaaaga atcagcgttt
    26161 aatcaggcag agtttaataa acttcttctt gagtgtgttg taaaaacgca gtcaacggtg
    26221 gcaaagattt taggtattga gtctcttagc cctcatgttt ccggaaaacc gaagtttgaa
    26281 tatgccaata tggttgaaga tatcagagag aaggtgtcgg ttgagatgga aaggttcttt
    26341 ccaaaaaatg atgatgaata aagtgaaata aattttgact ttgttcaaat atcatggttt
    26401 tttatggttc aaactgatga caataatcag aataattagt atattgatta tgtggttaat
    26461 gccacgttaa aaattgaaac tgaaaatcgc cgatgcaggg agcagccact ccctgcatcg
    26521 actgtccata gaatccttag aggaggttcc tatgtgtgcg atggatcgta gagaaagagc
    26581 actcatcagt caattgcatt tccctcatag tcttggagag gctatttcgt ttttaaaata
    26641 tccagtatgt gtccgcttcg aggatggttc ttttattcag gaaaataacc gctttgaaaa
    26701 acttatcaga tcgtcattta attcatgtga tgaatggttt gatagtttaa cgttggaatg
    26761 taaattacag ttatccagag cagagattga atcatgctct tcagtttatg gtgtaaattg
    26821 taataatgac atattactta ataatatgtt atggagtgtg atcatagaaa gtgttatcac
    26881 accatgcggc tatttcttta tctggagatt tatttgggta gcaaatgata atctgtcatc
    26941 gtttgttgtg tctaaataca ccaatgaatt aattgtacta tctgatgaat atgtaggcat
    27001 tgagccatat ctgatagggt ttagccatca ttattcatca gcaaagatga acatcactgt
    27061 gtctaaatca aagaagaaaa ccatgaaatt atttaaacga catggattta gttctagaga
    27121 tctctggttg gatgaaatga ttagaacgga aaa
//
LOCUS       DAZGVQ010000061        26974 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000061 DAZGVQ010000000
VERSION     DAZGVQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.061"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..74)
                     /locus_tag="ACHLAG_002445"
     CDS             complement(<1..74)
                     /locus_tag="ACHLAG_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein pndA"
                     /protein_id="HFP0655654.1"
                     /translation="MPQRTFLTMLIVVCVTILCFVWMV"
     gene            complement(307..1218)
                     /locus_tag="ACHLAG_002446"
                     /pseudo
     CDS             complement(307..1218)
                     /locus_tag="ACHLAG_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000893019.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            complement(1327..1479)
                     /locus_tag="ACHLAG_002447"
     CDS             complement(1327..1479)
                     /locus_tag="ACHLAG_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0655655.1"
                     /translation="MPQRTFLTMLIVVCVTILCFVWMVRDSLCGFRVEQGNTVLVATL
                     AYEVKR"
     gene            1811..2053
                     /locus_tag="ACHLAG_002448"
     CDS             1811..2053
                     /locus_tag="ACHLAG_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655656.1"
                     /translation="MSNEYQSTSINPDVFEQFFTLLNTHCKSSEAALAMLSTLCYELM
                     RRSGIHSLDSLMLNGEGILIQLQGADIREKTGQHIH"
     gene            complement(2146..3675)
                     /locus_tag="ACHLAG_002449"
     CDS             complement(2146..3675)
                     /locus_tag="ACHLAG_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP0655657.1"
                     /translation="MSCIKEKYAFERAKQYYFMLWCKESLIYLLFSVLVTWCCGYTVK
                     NTLGNVLILFLMLLLGRQNVISATCVCIFLLFSAFYAPVGMIYGKINNSFIASVMQTN
                     LNEAGEFITGIPVLYFIVSLFIAAFTFWFWRKRDKSHRGFIFLLFFIVLSVNAWPKRM
                     VTNVMTGIAETREEMMRYESLKHNQKDSWDIVNVEKKYKTIIIVIGESVRRDYLSVYG
                     YPLPTTPWLNSAPGIFINGYFSAAPNTIGSLSRTLTLDYTETGNPGNNIVTLARKAGY
                     ETWWISNQGSLGRHDTLISVIAANADKKYFLKEGSSSSRRIDDEDMLQYVREAVNSSS
                     PKVIFIHMLGSHPNPCDRLFNDAQPFRELFGNKVSCYLSTLLKLDRFLQEIYNMTTEH
                     GDDFAMLYFSDHGQSVSSDSTPVHHAPGLRSGYDVPLMMMASDITEHHLNHVHISARR
                     FPEIFQWITGIQTKNLPLSFSLLQNDFAESVSVFNGERDVPVMSLPEEPILTGNAESE
                     K"
     gene            complement(3875..4330)
                     /locus_tag="ACHLAG_002450"
     CDS             complement(3875..4330)
                     /locus_tag="ACHLAG_002450"
                     /inference="COORDINATES: protein motif:HMM:NF022214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HFP0655658.1"
                     /translation="MRDKSEVLTRAQKNRFGLILYIILLSVVYILITRLNLTTTYYSL
                     CLSLDENSQVTEYFIISIATFLFLAALSLFFIYSFFKRKRIVGKLLLCIYILYISHYA
                     ITFVYCLYFVGAECMTTGKIEHILIDIIIAMLSLSYVCLSGRARHIFVN"
     gene            complement(4418..5041)
                     /locus_tag="ACHLAG_002451"
     CDS             complement(4418..5041)
                     /locus_tag="ACHLAG_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0655659.1"
                     /translation="MQERIDYHIEKYQFRARNESPRLMRQWADVMRECRDTGAGARER
                     LRTALLNVDYVTSFELPFRLLLTRTPQMIDTLRREWLLSQKKALFNGRQRGCVYSLQN
                     DLSGVPDCFSYSLATRIRRMDCYGMTTRCFTDIAGQVKAPTARLKLALESGLLVTALD
                     GLFWLGTQRIAADVQRLRQSGMVIVTREVEVSDTLTGTTRLVSAYAL"
     gene            5190..5672
                     /locus_tag="ACHLAG_002452"
     CDS             5190..5672
                     /locus_tag="ACHLAG_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFP0655660.1"
                     /translation="MIKYIFALTLLLSGSVCADQIQGLVIRVLDGDTLEILQARHPVR
                     IRLVNIDAPEKKQPFGRWSMNQLKSLVVNTPVTVTYTQTDRYGRILGRVYTEDGMEVN
                     RWLVQHGAAWVYPDFNTDYTLPAYQREARTMKQGLWADKKPVPPWLWRKENKIAQDCK
                     "
     gene            6523..10428
                     /locus_tag="ACHLAG_002453"
     CDS             6523..10428
                     /locus_tag="ACHLAG_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0655661.1"
                     /translation="MNKVYSLKYSHITGGLIAVSELTRRITTGSRKKLFAIISLSLIG
                     VITPSVASQMDLTNFYSQDFFDFAQNKGAFKTGAKNITILKKDGSTLELPDVPFPDFS
                     SVSNKGSTTAIGGSYTVTATHNNISHHSISTQGFGQTNYSYIDRMSKGDFAVTRVDKF
                     IVEGTGDIESADISLSKNEALERYGIMYKGKKQLIGFRAGAGSLIFMKNGKTTNNQNA
                     SYAPDIRNGSFVLIDGWEGERITTNNLFDEFKNRTTGGDSGSSLFVYDNEKNKWVILG
                     TLFGEYHYSNGQIRSAFNKYDQSLVNKLKDYFTQQINLSGKSAVIKNDNITINSSSDS
                     IKVVNQLKNNKTVQMDLSFNGGGTINLEQDLHTGSGGLIFDDSSVYEINGNNYSYKGA
                     GINTGNSSVVNWNVKGIKGNDLHKIGKGTLNIKSTQDGNLKIGDGKVVLQAEKSFDNI
                     YITSGKATVVIDKNNALNNENEFSGFYFSKNGGVLDLNGHDQSFKKIAATDNGATITN
                     TSEKTATLSLSSTSDYLYHGNISNNIQIRHEHTGKKSDSRLILDGDININNDIHIKNA
                     QVVMQGHATSHTVVKNQACGLPSFLCPVTTTAHLSNLEKNDALKNGVAYKANNQSASF
                     EQPDWEDRQFSFRTMTLDNSVFTTSRNSFLKGDIISSDSDIYFGSKDDVYIDTLAGEN
                     ITGNGFSFSQNIKKGVSVGDSGFSGGIDSTDGSIRIGDKANVTLTKSSSMDNTTLTIE
                     KGGLINAQGGLFTSKESLIAGQMNLTGKPVLNTNAWSPSMYLGFGGYKLTEDGAQLSA
                     RNQASVIADIYSDKTANISLGKENNTESKNTPAYSSFAISLLNGFDTSLEGKINAAKS
                     TLTMNDALWKVTGDSALKNMSTTGSMVLFSNENRNSFNKLTVDELTTNNSAFVMRTNT
                     QQTDQLIVKNKLEGANNLLLVDFIEKKGNDKNGLNIDLVKAPENTSKDVFKTETQTIG
                     FSDVTPEIKQQVKDNNSVWTLTGYKTVANASAAAKATSLMSGNYKAFLNEVNNLNKRM
                     GDLRDINGEAGAWARIMSGAGSAGSGYSDNYTHVQIGADKKHELDGLDLFTGLTMTYT
                     DSHAGNHDFSGETKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYS
                     THSWYAGAEAGYRYHVTEDAWIEPQAELVYGAVSGKQFAWKDQGMHLSMKDKDYNPLI
                     GRTGVDVGKSFSGKDWKVTARAGLGYQFDLLANGETVLRDASGEKRIKGEKDSRMLMS
                     VGLNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            10925..11290
                     /locus_tag="ACHLAG_002454"
     CDS             10925..11290
                     /locus_tag="ACHLAG_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655662.1"
                     /translation="MNDGRQTPPSPEAWKIRFLANGTPGTWFYTDDAAVTKEWLRAHC
                     DDVEVIPLCRMHDGPVFREHHINELSAALARMLHMHHILLERTRYRENIHDAECIREI
                     HEAPLQAIGILEKYKRTKS"
     gene            11300..11491
                     /locus_tag="ACHLAG_002455"
     CDS             11300..11491
                     /locus_tag="ACHLAG_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655663.1"
                     /translation="MWILIFWLSSPFNVSSGDARQPTLAVHSQEFRTEKACRDAFTAL
                     KKENVGEFALRGVCVPAGE"
     gene            11525..11848
                     /locus_tag="ACHLAG_002456"
     CDS             11525..11848
                     /locus_tag="ACHLAG_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFP0655664.1"
                     /translation="MAYHPPDITLHYPYLRRMSWAQITAAAEDAEIHHDYARALILWQ
                     HAYHAATLTLNKNLAVAKIDFCIKRIRMHEQMSRIIRKTGTDEILFRLSKNHHLYEKK
                     KTKEG"
     gene            complement(11994..12089)
                     /gene="dqlB"
                     /locus_tag="ACHLAG_002457"
     CDS             complement(11994..12089)
                     /gene="dqlB"
                     /locus_tag="ACHLAG_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFP0655665.1"
                     /translation="MINLLIIVLRAVVAVVNAVIALLELIRELID"
     gene            12647..13918
                     /gene="trbA"
                     /locus_tag="ACHLAG_002458"
     CDS             12647..13918
                     /gene="trbA"
                     /locus_tag="ACHLAG_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HFP0655666.1"
                     /translation="MSYQQTSAAEDPMAIWYIVGAICLLFAIIIWRFLPEIVFASCLI
                     LHTLWGMIDWGPFHNFAAPRYNLLAITANNAATITFSQWLDVMSRTVGILWLILLPMT
                     FGFLWMWFHHPAQPRFTRRPLNIHTLPHIFSALSPAIAPVLADGDNNRLFHGQKRPER
                     RVALPPEAFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQKWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEACRLGLPCPEPCVEEAIEGLRQDMLRLGLIWDEPQPDRDRRRQIRTRWSLTDDVI
                     PRRHDNDEGSETGETTETTETRHPADKEKAQ"
     gene            13915..15024
                     /locus_tag="ACHLAG_002459"
     CDS             13915..15024
                     /locus_tag="ACHLAG_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFP0655667.1"
                     /translation="MTIRYTATLNNKHLSLTALYPDGGQEVIAILPVTELSALINRNN
                     RLMHLSFSTPFREITETLLVDTVDTERPGHHIGRMLSAIDSCLNAAYAELPARKTSFM
                     RGLIYGAGLVLALLTAVTAGYILLKPDSDSPLRVPVTESVTETAPLHIDKEIVRSVAP
                     SETKNEIVHPAPQEMQETSAAPAITADKNTLVAEARRNLATVLKRNADRGMFTINLST
                     GHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGEASTNQTAPLLCEPD
                     AQKRATGWHQLYSADGGMAPTGDAPDEKCLTAARAAIDVNNQAFRQFGFGGTPWVLSD
                     TGWHLPSGILKDATTVSLFLKATDTEDNHNEPASR"
     gene            15005..17308
                     /gene="trbC"
                     /locus_tag="ACHLAG_002460"
     CDS             15005..17308
                     /gene="trbC"
                     /locus_tag="ACHLAG_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFP0655668.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNIIGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFTLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEVIQSGDKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVRERK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFITQQSMILARDLGYRLEGTDAQTLEVKKYKGRFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKINVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTEKTSRKRRKKHTEPYRIIDTFQDQLKRTQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            17855..18004
                     /locus_tag="ACHLAG_002461"
     CDS             17855..18004
                     /locus_tag="ACHLAG_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655669.1"
                     /translation="MNSQILPDMLINFALINITDRKNEGINTIDGNWQADEGRRYRDN
                     VRIYF"
     gene            complement(18102..18533)
                     /locus_tag="ACHLAG_002462"
     CDS             complement(18102..18533)
                     /locus_tag="ACHLAG_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFP0655670.1"
                     /translation="MVLRSIWIEEQYMGVKKIVILAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(18601..21312)
                     /locus_tag="ACHLAG_002463"
     CDS             complement(18601..21312)
                     /locus_tag="ACHLAG_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFP0655671.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGWKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRTEPQAGPFE
                     MAAADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAVCRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVIICEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(21324..21656)
                     /gene="mobA"
                     /locus_tag="ACHLAG_002464"
     CDS             complement(21324..21656)
                     /gene="mobA"
                     /locus_tag="ACHLAG_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFP0655672.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            21889..22224
                     /locus_tag="ACHLAG_002465"
     CDS             21889..22224
                     /locus_tag="ACHLAG_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFP0655673.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKAFRDIIREAESKKTSRRSSSKTAPKKTDSAR
                     KDGIDMNNN"
     gene            complement(22309..23157)
                     /locus_tag="ACHLAG_002466"
     CDS             complement(22309..23157)
                     /locus_tag="ACHLAG_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP0655674.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGISLFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRRMDKPEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVWRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRS
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            23505..23702
                     /locus_tag="ACHLAG_002467"
     CDS             23505..23702
                     /locus_tag="ACHLAG_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655675.1"
                     /translation="MQGAPHPARLKKNDGTTMACRGHWCTTPAQVKTEKTTRHIQSKK
                     VKFLHFFTLFYRADLVTMMIK"
     gene            23736..23987
                     /locus_tag="ACHLAG_002468"
     CDS             23736..23987
                     /locus_tag="ACHLAG_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP0655676.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIWRLENELGETAEDV"
     gene            complement(24018..24251)
                     /locus_tag="ACHLAG_002469"
     CDS             complement(24018..24251)
                     /locus_tag="ACHLAG_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655677.1"
                     /translation="MRVSQPGKMPGFLSSRSRANSMTLAERLIQKGFEKGALEVAREA
                     ACRLRDMGWPPERIQEATGLSGEELKKLFPDEQ"
     gene            complement(24251..25177)
                     /locus_tag="ACHLAG_002470"
     CDS             complement(24251..25177)
                     /locus_tag="ACHLAG_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP0655678.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPASFVEPDLHQYASDILWSVKTTGGKDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFEEPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGRMEGALEKAL
                     AIACQLQKMGMTPEQIRQATGLSDDELKKITH"
     gene            complement(25174..25563)
                     /locus_tag="ACHLAG_002471"
     CDS             complement(25174..25563)
                     /locus_tag="ACHLAG_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFP0655679.1"
                     /translation="MKSCFDTDKDFQMSSDEVTFNADSVRSKLELLSEELMHRYGLVY
                     ELYQRRLSAAIDNFVSTLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDAERGYCCHG
                     FDRNCCPLGCGDLDDDCCDAADLMEEP"
     gene            complement(25754..26020)
                     /locus_tag="ACHLAG_002472"
     CDS             complement(25754..26020)
                     /locus_tag="ACHLAG_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655680.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRVRRQELAPDEIN
                     PDVLAMGGHIARRCSERKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(26112..26546)
                     /locus_tag="ACHLAG_002473"
     CDS             complement(26112..26546)
                     /locus_tag="ACHLAG_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFP0655681.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQLA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFMEAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
BASE COUNT         7421 a         6941 c         6005 g         6607 t
ORIGIN      
        1 ctcaccatcc agacaaaaca cagtatcgtc acacagacga caattaacat tgttaaaaac
       61 gttcgttgtg gcatcgcagc ccctccattg cctttcggcc gtcatcgggc tactataatg
      121 ttgttgaggc attacagtgg gccctgggtt gattgataaa aagatcacct ggggctttct
      181 gctgcatgcc gtccacaaaa cgctttcggc atacagcctc aagcgcccgc cgctattcta
      241 attgtttttc agtgagccac aatcgttttc aactttctga cttacagtag attacctgca
      301 catgattcag gaaaacatcg acaatcggga tgccactgcc tgcactgcat ctcttccagc
      361 ctgacttata ccgccaagct gaaagaaacc atgaataacc cccagatatc gctggcacgt
      421 acacattacg ccctgctcag tcattcgctg atacaacatc tccccttcct gccgtaaagg
      481 atcaaattca gctgtgataa tatgagtagc aggcagtccc tgtaaatccg aacgccagag
      541 aggcgaggct tcaggatgtt cacgatccgt atcatgtaag tacatatcat aacctgatac
      601 aagtgcatct cgcgtaataa catagtcatc accaaacatg gcataacttt ccaggcatgc
      661 tgttgcatca agcatgggat aaataagaat aagttgtgaa ggttgccact gagctactga
      721 tttaaggcgt aatgccgtta caagagcaag atgacctcca gcactgtcac ctgccagaga
      781 aatcctgttc cggtcaattc ccagagattc cgcattctgc catatgatgc aggctcctct
      841 ctcagcatcg tcgtgcgcca cgggaaacaa aaactcagga gcaagacggt actgaactgc
      901 gataacacga caaccactat aataagccag ttgtcttagc tggttatcat gggtctcaaa
      961 cccaccactg acgaagcaac cgccatgata ataaataaga catggagtga tatctgatat
     1021 atctggtcga acaaaaatcc ggaatcgcat tccgtctata aaaatatcgc tgacattaac
     1081 ccgttcttct ttttttcccg caagacaagt gctggtaata taaccagctc ttctgtcatt
     1141 aatatgttac atccggtaag aaggacgccc ggatgtaata aaatcttcca ctaactgaga
     1201 aataccttgt tccagcataa cgtcaggtcc tgtaattccg ggaataacga cgaataggaa
     1261 aaagggcgct gtgaggtctg ccttcgcaac aatccggcgc cagatttcct gccgccgtgt
     1321 tgcccgttaa cgtttaactt cgtaggctaa cgttgccaca agcactgtgt ttccctgctc
     1381 gacacggaat ccgcaaagcg aatccctcac catccagaca aaacacagta tcgtcacaca
     1441 gacgacaatt aacattgtta aaaacgttcg ttgtggcatc gcagcccctc cattgccttt
     1501 cggccgtcat cgggctacta taatgttgtc taggcattac agtgggccct gggttgattg
     1561 ataaaaaatc gcctggggct ttctgctgca tgccgtccac aaaacgcttt cagcatacag
     1621 cctcaagcgc ccgccgctat tctaatcgat tttcagtctg ccacaatcgt tttccaccta
     1681 atgacatgtt atgcacaaca gtatttttac gggaaactac acggcaagga ttcccggaac
     1741 acgttcatta ttttatggtt acagacatca tgttaaatct ggaagtaata atgacctgga
     1801 aggaaatact atgagtaacg aatatcaaag caccagtata aacccggatg tttttgaaca
     1861 attttttacc ctcctgaata cacactgtaa atcatcagaa gctgcacttg ccatgctgag
     1921 tacactctgc tacgaactga tgaggaggag tgggatccat tcactggaca gcctgatgct
     1981 caacggagag ggtatattga tacaattaca gggggctgat attcgcgaaa aaacaggtca
     2041 gcatattcac tgatccaggt atgggcttaa aacagtgtca gcctgaaata aacgctcaat
     2101 gcctcataca catgaggcat tgagcacggt cataaataat ctttattatt tttctgattc
     2161 agcatttccg gtcagtatcg gctcttctgg caaagacatt accggaacgt cacgttcacc
     2221 attgaataca gaaacacttt cagcgaaatc attctgtaac aaagaaaaac ttaacggcag
     2281 atttttggtt tgtatccctg tgatccattg aaaaatctcg ggaaaacgac gtgcactgat
     2341 atgcacatga ttgaggtggt gctcagtaat atcactggcc atcatcatga gaggtacatc
     2401 gtatcccgaa cgcaaacctg gagcatgatg cacaggggtg ctgtctgaac tgacggattg
     2461 cccatgatca gaaaaataca gcatggcaaa atcatcgcca tgctctgtcg tcatgttata
     2521 aatttcctga agaaaccgat ccagcttaag caatgttgat aaataacagg atactttatt
     2581 tccgaataat tccctgaacg gctgtgcatc attaaaaagt ctgtcacagg gattaggatg
     2641 ggaccccagc atatgaataa aaatcacttt tggagaagac gaattaacag cttccctgac
     2701 atactgaagc atatcttcat catcaatgcg acgggaagaa gagctccctt ccttaagaaa
     2761 atatttctta tccgcattgg cggcgatgac agaaatcagc gtatcatgtc gccctaaaga
     2821 gccctgattt gatatccacc acgtctcata tccggctttt ctggcaagcg taacaatatt
     2881 attacccgga ttacctgttt cagtgtaatc aagagtcaga gttctgctga gtgaacctat
     2941 cgtattcgga gctgcggaga aatacccatt aataaatata cccggagcag aattcaacca
     3001 tggtgttgtc ggcaaaggat acccataaac agaaagataa tcccgtctga cactttcacc
     3061 aattacgata attatggttt tatatttctt ctccacattc acaatatccc atgagtcttt
     3121 ctgattgtgt ttcagcgatt catatcgcat catttcttct ctggtttcag ctatccctgt
     3181 cattacattc gtaaccatac gttttggcca tgcatttact gacaatacga taaagaacaa
     3241 cagaaagata aatccccggt ggcttttatc acgcttacgc cagaaccaga atgtaaaagc
     3301 agcaatgaac agtgaaacaa taaaataaag aacaggtatg cctgtgataa attcacctgc
     3361 ctcatttagg tttgtctgca ttacagacgc aataaagctg ttgtttattt ttccgtaaat
     3421 catcccaacc ggagcataga atgcactaaa caaaagaaag atacagacac acgttgctga
     3481 aataacattc tgccgcccca acaacaacat caggaacaat ataagcacat tccccagggt
     3541 gtttttcact gtatatccgc aacaccatgt aacaagcaca gaaaacaaca gataaatcag
     3601 tgactcttta caccacaaca taaaataata ctgttttgct ctctcaaaag catatttttc
     3661 tttaatacaa gacacgccag tcattttcct gtacaaacac agacgatcat ttcaacgcat
     3721 ggatatttat atgaaacagt tcacaaaaat accctatcct gtccagcagg atttacatat
     3781 tatcaggttc tgtgtttaca gcaactgctc cccgagccag attattcgca taacccgatg
     3841 aaggcctcat aaccataaga cactcagaat acatttagtt aacaaaaata tgtcttgccc
     3901 tcccggaaag gcaaacatac gaaagagaaa gcatggctat aataatatca atcaaaatat
     3961 gctcaatctt tccggttgtc atacactcag caccgacaaa ataaagacaa taaacaaaag
     4021 tgatagcata gtgtgatata taaagaatat aaatgcacag aagtaatttc ccgacaattc
     4081 ttttccgttt aaagaaagaa taaatgaaga acaaagataa agccgccaga aaaagaaatg
     4141 tagctatgga tattataaaa tactccgtca cctgtgaatt ctcatccaga gataaacaca
     4201 gcgaataata tgtggttgtg agattaagtc ttgttatcaa tatataaaca actgataata
     4261 gtataatata aagaatcaga ccaaatctgt ttttctgtgc tctggttaat acttctgatt
     4321 tatctcgcat aaaatatcct gtctgtaatc agtcccggtc aacaatggta aacttcagaa
     4381 tcagtacgaa tacggtaaca aagattcccc cagtgcttta aagtgcatag gcagaaacca
     4441 gtctggtggt tccggtcagg gtatctgaca cctcaacttc ccgggtgact atcaccatac
     4501 ctgactgacg cagtctctga acatcagctg caattcgctg agttcccagc cagaacaggc
     4561 catccagtgc cgttaccagc aacccgcttt ccagcgccag ttttagtcgt gccgtcgggg
     4621 ctttaacctg cccggcgata tctgtgaaac aacgtgtcgt catgccatag cagtccatcc
     4681 gccgtattcg cgtcgcaaga ctgtagctga aacaatccgg cacgccggat aagtcattct
     4741 gcagactgta tacacatccc cgctgccgcc cgttgaacag agcttttttc tgactgagca
     4801 gccattcccg acgcagagtg tcaatcatct gcggtgtccg ggttaacagc agacgaaacg
     4861 gcagctcaaa actggtcaca taatcaacat tcagtaatgc tgtccgcagt cgctccctgg
     4921 cacctgcccc ggtatcccgg cattcacgca tgacatcagc ccactgacgc atcagtcgtg
     4981 gagactcatt cctggccctg aactgatatt tctcaatatg ataatcaata cgttcctgca
     5041 tatcgccacc agatatctgt tgtttaccca gacacagtga ttagcctaca ggaataaata
     5101 tcctccgtga agccaagtgc ctcagaagat aaaaacatgt acttttttca acgtgtaata
     5161 ttaacaccac caccatagga aaatataaaa tgataaaata tatattcgca ctcacacttc
     5221 tgctttccgg gtctgtctgt gccgatcaaa ttcagggact cgttattcgt gtgctggacg
     5281 gagatactct ggagatatta caggcccggc atcctgtgag gattcgtctt gttaatattg
     5341 atgcaccgga aaagaaacag ccttttgggc gctggtcaat gaatcaactt aaaagccttg
     5401 tggtaaatac gcccgtaacc gtgacataca cacagacaga ccgctacggg cgtattcttg
     5461 gacgggtata taccgaagac ggcatggaag ttaaccgctg gctggtacag catggtgcag
     5521 cctgggtata tcctgatttc aacacagatt acaccctgcc ggcataccag agagaagcca
     5581 gaacaatgaa acagggtctg tgggcagaca aaaaacctgt tccgccatgg ctgtggcgaa
     5641 aagaaaataa aattgcacag gattgtaaat aaaacagctc acaggcagtt tttgcggact
     5701 atcgccatac aggtgaaacc acgtaccaga agcgggagca aagcagggcc aaacctaacg
     5761 ctcaggctgt tttactgatc cggatagtgc ggaagaatcc ggaaacgtta aatacacttg
     5821 ccctgcttta aataatacag gaaacaggga gatgtaaggt tagatcagct tccgcaacaa
     5881 tccgacagca tatttccttc taccgtgttt cctgttaacg ttgatcttag taaaaatggc
     5941 aactgtcaaa gatcgtgaca ttgacaaact ttgtgagcta acgttgccat aggcaccata
     6001 tttccctgga ggaaacagaa tccgcaaagc gttcgttgtg gcatcacaat ccatccattg
     6061 cctttcaact gttatacctg gctactatga tgttgtcctg acattacagc agagttccgg
     6121 attgaacgat aaaacatcgc cagggaactt ctactgcatg ccatctacaa aacacttttg
     6181 gcatacaaca tgagaactat cccacacaaa ggcatcagaa tactcccccc ctgaattaat
     6241 aagaaatatt aatcaatgtc gcatctgtat gctttatcct tcccggtacc aggcatactt
     6301 taaaaaataa aaaacatgat aaaaaactac cgcccctcct tttttttgct gagttcccgc
     6361 tgaagaatcc ataatggaca tgatgtattt gttatctgca tcataacaaa ctcaccaaat
     6421 gctaaacatt tgtctgaaaa atgattacaa tcataaaact gtcagagtcc ccctctaaca
     6481 gattaacaac aataatactt ttcctaacat actggagctt atatgaataa agtatattct
     6541 cttaaataca gtcatattac cggaggatta atcgcagtat ctgaattaac gcgcagaata
     6601 acgacaggat ccagaaaaaa actatttgct attatttcat tatctctgat tggtgtaata
     6661 actccctcag ttgcatctca aatggacctt acaaattttt attcgcagga tttcttcgat
     6721 tttgcacaaa ataaaggagc attcaaaaca ggagcaaaaa acatcactat tctgaaaaaa
     6781 gatggctcta cccttgaatt accagatgta ccattccctg atttttcatc tgtatcgaat
     6841 aaggggtcaa ctactgcaat tggtggttca tatactgtta ctgcaaccca caataatata
     6901 tctcatcact ctatatcaac ccaggggttt gggcaaacaa actattcata tatagacaga
     6961 atgagtaaag gtgactttgc cgttacacga gtggacaaat ttattgttga aggcactgga
     7021 gatatagaga gtgctgatat cagtttatct aaaaacgaag ctcttgaacg atatgggatt
     7081 atgtataaag ggaaaaaaca actaattggc ttcagagctg gagcaggcag tcttattttt
     7141 atgaagaatg gaaaaacaac aaataaccag aatgcaagtt atgctcctga tatcagaaat
     7201 ggttcttttg tactgattga tggctgggaa ggcgaacgca tcaccaccaa taatcttttt
     7261 gatgaattta aaaacagaac cactggtggt gacagtgggt cttctctttt tgtatacgat
     7321 aatgaaaaga ataaatgggt tattcttggt acattatttg gtgaatatca ttacagcaat
     7381 gggcagatcc gttctgcatt caacaaatat gaccagtctc tggtgaacaa gttgaaagat
     7441 tattttactc aacagataaa cctgtcaggt aaaagcgcag tcataaaaaa tgacaatata
     7501 actatcaact cctcatctga cagtattaaa gttgttaacc agctcaagaa taataagact
     7561 gtgcaaatgg atttatcttt taacggtggt ggaactataa atcttgaaca ggatttgcat
     7621 acaggttctg gagggcttat ttttgatgat tccagtgtat atgaaataaa cggaaataat
     7681 tactcataca aaggtgctgg tattaacacc ggaaatagct ccgttgttaa ctggaatgta
     7741 aaaggaatta aaggtaatga tctccacaaa ataggtaagg gcacacttaa tataaaatca
     7801 acgcaggatg ggaatttaaa gatcggtgac ggcaaagttg ttttgcaggc tgaaaaatca
     7861 tttgacaaca tttatataac cagcggaaaa gcaacagtcg ttatcgataa aaacaatgca
     7921 ttaaataatg aaaatgagtt cagcgggttc tacttttcta aaaatggagg agtgctggat
     7981 ctgaatggac acgatcaatc ctttaaaaaa atagctgcaa ccgacaatgg tgctacaata
     8041 acaaacacat cagaaaaaac agccacctta tctttatcca gtacatccga ctatctttac
     8101 catgggaata tcagcaataa tattcagata cgccatgaac atacaggtaa gaagagtgat
     8161 tcacgactca ttcttgacgg agatataaat ataaataatg acatacatat taaaaatgcg
     8221 caggttgtga tgcagggaca tgccacatcc catactgttg taaaaaatca ggcatgtggt
     8281 ttaccatctt ttttatgtcc ggttacaaca accgcccact tatccaatct tgaaaaaaat
     8341 gatgcattaa aaaatggggt tgcgtataaa gcaaataacc agtcagcatc atttgaacaa
     8401 ccagactggg aagatcgcca gttctcattc agaaccatga ctctggataa ttcggttttt
     8461 acaacctctc gtaactcttt tctgaaagga gatattatct catcagactc tgatatctat
     8521 tttggaagca aggatgatgt atatattgac actctcgctg gtgagaacat caccgggaat
     8581 ggattcagtt ttagccagaa tattaaaaaa ggcgtatctg tgggtgattc cggttttagc
     8641 ggggggattg actctactga tggtagtatc aggattggtg ataaagcaaa tgtcaccctg
     8701 actaaatcca gttcaatgga caatacaact ctgactatcg agaaaggtgg tttaataaat
     8761 gctcagggtg gcctgttcac cagtaaagag tcactgattg cgggacagat gaacctgaca
     8821 ggtaaacctg ttctgaatac aaacgcctgg tctccatcca tgtatttagg ctttggtggc
     8881 tacaaactga ctgaagatgg ggctcaactc tcggccagaa accaggcatc tgtaattgct
     8941 gatatttact ctgataaaac agcaaatatc tcacttggta aggaaaacaa tactgaaagt
     9001 aagaacactc ctgcttactc atcatttgcc atatcattat tgaatggatt tgacacctca
     9061 ctggaaggaa aaattaacgc agccaaatcc acactcacaa tgaacgatgc gttgtggaaa
     9121 gtgaccggtg attctgctct gaaaaacatg tcaacaaccg gcagtatggt tctcttcagt
     9181 aatgaaaacc gtaactcatt caataagctg actgttgatg aactgaccac caacaacagc
     9241 gcctttgtga tgcgaactaa tacacaacag accgaccagt taattgttaa aaacaaactg
     9301 gaaggtgcaa acaacctgct gttagtcgat ttcattgaga aaaaaggaaa cgacaaaaac
     9361 ggtctgaaca tcgacctagt taaggctccg gagaatacca gtaaagatgt attcaaaact
     9421 gaaacacaga ccattggttt cagtgatgtg acgcctgaaa tcaaacaaca ggtaaaagac
     9481 aataactccg tatggacgct gactggctat aagactgtcg ccaatgcgag tgctgcagca
     9541 aaagcaacat ctctgatgtc cggcaactat aaggccttcc tgaatgaggt caacaacctc
     9601 aacaaacgta tgggtgacct ccgtgacatc aacggtgaag ccggtgcatg ggcacgtatc
     9661 atgagcggcg ccggctctgc cggtagcggt tactctgata actacaccca tgtacagatt
     9721 ggtgcggata aaaaacatga acttgatggc cttgacctct tcaccggtct gaccatgacc
     9781 tataccgaca gccacgccgg aaaccatgat ttctccggtg aaaccaaatc tgtcggggca
     9841 ggtctctacg cttctgccat gtttgattcc ggtgcctata tcgacctgat tggtaagtat
     9901 gttcaccatg ataatgagta caccgcaacc tttgccggac tcggaacccg tgattacagc
     9961 acacattcat ggtatgccgg tgcagaagcg ggctaccgct atcatgtcac tgaggacgcc
    10021 tggattgagc cacaggcaga actggtttac ggtgccgtat ccggtaaaca gtttgcatgg
    10081 aaggaccagg ggatgcatct gtccatgaag gacaaagact acaatccgct gattggtcgt
    10141 accggtgtgg atgtgggtaa atccttctca ggtaaggact ggaaagtgac agcccgtgcc
    10201 ggtctgggct accagttcga cctgctggct aacggcgaaa ccgtattgcg ggacgcatca
    10261 ggtgaaaaac gtatcaaagg tgaaaaagac agccgtatgc tgatgtccgt tggcctgaat
    10321 gcagaaatca gggacaacgt ccgctttgga ctggagtttg agaaatccgc ctttggtaag
    10381 tacaacgttg ataatgctgt caacgcaaac ttccgttact cgttctgata ctccgtattc
    10441 aaaagggcct gcgggccctt tatgctcagg acctgccgtc ctggtataca tttccggggt
    10501 atcagtcgat ttgtttactt aactgcgatg actaaagctg gcagtaatgc cggctttttt
    10561 acgaccacac acgtcttcct ctcatccgga actgtgatac aaatcctgca tacaggattg
    10621 atcgccggtg cccacaaatg gcacattttc agtaagattc tgaatgttac attccccctt
    10681 catgtgtggc acagctgtca ccgggaaaga aacgaaaagc acatgaaaaa cataaaaact
    10741 cacaccggac tccttatcca caaagaacaa gcgcggcgcg ttcgtcttca tgaaactccg
    10801 gccgcctggt gccacacgca ccgggaatgc tacagcaaaa cgaccggtcg ccgctgcggc
    10861 agccctgaca gtctcagcag gctgattctg agcagcattc agcccataga atgagatgac
    10921 ccgtatgaat gatggcagac agacgcctcc gtcccctgag gcatggaaaa tccggtttct
    10981 ggcgaacggt acacccggca catggtttta caccgatgac gcagccgtca caaaagagtg
    11041 gctgcgggca cattgtgacg atgtggaagt gatccccctg tgccgtatgc atgacggtcc
    11101 cgttttccgg gaacaccata ttaatgaact cagcgccgca ctggccagaa tgctccatat
    11161 gcaccacata ctgctggaac gaacccggta cagagaaaat atccatgatg cagaatgtat
    11221 tcgtgaaatt cacgaagctc ccctgcaggc tatcggcatc ctggaaaaat acaaacgcac
    11281 aaaatcataa ggcagttcta tgtggatact gattttctgg ctctcatccc cctttaatgt
    11341 ctcctccggc gatgcccggc aacccacact ggccgttcac agtcaggagt tccgtactga
    11401 aaaagcctgt cgggatgcct tcaccgcgct gaaaaaagaa aacgtgggag aattcgctct
    11461 ccggggagtc tgcgtccccg ccggagaata aagcggagat gatgattagc cacaggagta
    11521 ttcaatggca taccatcccc cggatatcac actgcattat ccgtatctgc gccggatgtc
    11581 ctgggcgcag ataacggcag cagctgaaga tgcggaaata catcatgatt atgcccgggc
    11641 attaatattg tggcagcatg catatcatgc tgccacgctg actctcaaca aaaacctggc
    11701 agttgctaaa atcgacttct gtattaaacg tatccggatg catgaacaaa tgagcaggat
    11761 aatccggaag actggcacag acgaaatact cttccgtctg agtaaaaacc atcatttata
    11821 tgagaaaaag aaaacaaaag agggatgaca gtcccattgt atgggaaaaa cttgccatgc
    11881 ttactgcagg tgcatttttg atactggcag tcctgggtga gtaatcttaa tatcctgttt
    11941 attaaagggc agaaggcggt ccccctctgc ccttaagtac gtttccggga ttatcagtca
    12001 ataagttccc ggatcagttc cagaagagct atcactgcat tgaccacagc aacaaccgcc
    12061 ctgagaacga tgattaacag gttaatcatc cgtttctcca tcaatggagg aagtcaccgt
    12121 tgcttgctca tacatcgcct gtcacaacgg ctctctgaca ttgttggatg taaagtacct
    12181 tcccccctgc cagagcactg aacacagcac cacctgtttg ttcagccagg actttaattc
    12241 atccatacac agatagaaca ggttttagaa gcaagggagg cactctgtac agcgccaaca
    12301 caaatattat acagatgcac acataaaatt catttaaaga aatgaatatt gacaggcaat
    12361 ctgaagtcaa actagacaac aaatgtctgg tgccataata ttatggatgt tggatgtaga
    12421 gtatcttcct ccggtcagag caccaactcc gctccggaat agcaaaaaaa ccggcctcac
    12481 cagtcggttt ttttgtgcct gtctcccacc accttctgcc gttcgatctt ctctgtgaaa
    12541 atgtcctgtt gaactcattc ctgtccggtt attaccggcg ctgattcgca ttcatgccac
    12601 ccgacttaat cccgaaattt atccattcat ctatggagga atccccgtgt cttaccaaca
    12661 gacgtctgcc gccgaagatc caatggctat atggtatatc gtcggtgcca tatgcctgct
    12721 ctttgcgatt atcatctggc gctttttacc tgaaatcgtc ttcgcctcct gcctgatcct
    12781 gcacacactg tggggcatga ttgactgggg gccctttcac aactttgccg ccccccgtta
    12841 caacctgctg gccatcactg ctaacaacgc ggcaaccatc actttcagcc agtggctgga
    12901 tgttatgtcc cggacagtgg ggatcctctg gctgattctg cttcccatga cattcggctt
    12961 tctgtggatg tggtttcacc atccggccca gccccgtttt acccgcaggc cactgaatat
    13021 ccacacactg ccccatattt tttcagctct gtcaccggcg atcgcccccg ttctggctga
    13081 cggagacaac aacagactct tccacggaca aaaacgtccc gagcgccgtg tcgcactacc
    13141 ccccgaagcc tttgtggagc agaacaacct tatccgcaac atgcagctcg atgttgcgtc
    13201 cacacgccag tgcttcatgg cgcaactggg gcaaccactg accagctgga aagacatggc
    13261 cccacatgaa aaagcgctct ttgccatctt tggcctgcag ttcttcctcg gtgaccggaa
    13321 agcagcagta gccctgatga acaacctcaa tctctcctgc aggctcaaaa gtaaacggga
    13381 ccagggacgt ttcagcacgc cggtgtattc actggccaga aacgccttta tccgggtcat
    13441 caaaacagaa ggcgcacaaa agtggctcag gcagcaccgt tacgttcgca gcggtctggt
    13501 ctggctttac gcacatgatt tgcgcctgac gcctccgaac tggctgtggc tcaagggcgt
    13561 tgaccggacg ctcttctatg cccttcaccg ggcaaacacc acaaaaggct tcatcgaagg
    13621 ggccggcgtg gtggccgttg cccgggcaga aaatgaagcg tgtcgtctcg gcctgccctg
    13681 cccggaaccc tgtgtggaag aagccattga agggctgcgg caggacatgc tgagactggg
    13741 gttgatatgg gacgaacctc agccagaccg cgatcgcaga cgtcagataa ggacccgctg
    13801 gtcactcacg gatgacgtga taccacgccg ccatgataac gatgaaggct cagagactgg
    13861 tgaaaccacc gaaaccactg aaacccggca tcccgccgac aaggaaaaag cacaatgacc
    13921 atccgctaca ctgcaacact gaacaataaa cacctgtccc tgaccgccct gtaccctgac
    13981 ggtggtcagg aggttattgc catcctgccg gttacagaac tgagcgcact gattaaccgt
    14041 aataacaggc tgatgcacct gtccttcagc accccttttc gtgaaataac cgaaacactg
    14101 cttgttgata ccgttgatac tgaacgtccc ggacatcaca tcggcagaat gctctccgcg
    14161 attgacagct gtctgaacgc ggcatatgca gaacttcctg cccggaaaac cagcttcatg
    14221 agagggctga tatacggtgc aggattagtg ctggcacttc tgacagcggt aacagccgga
    14281 tatattttac tgaaaccgga cagcgattcc cctctcaggg tacctgtcac agaatccgta
    14341 acagaaactg caccattaca catcgacaag gaaattgtcc ggtcagtagc cccgtcagag
    14401 acgaaaaatg aaatcgttca cccggcacca caggaaatgc aggagacatc cgctgccccg
    14461 gcgataacag cagacaaaaa tactctggtc gctgaagccc gccgtaatct ggccactgta
    14521 cttaaacgca acgcggaccg gggtatgttc accattaatc tctccactgg tcatgaacgc
    14581 acactctacg ccttccttga ccccgcatgc atcaactgcc gtcttctgga gcccgcgctt
    14641 aagcaactgg cccgggaatt cagcgtggtg atttatccgg tctccgtaat cggcggagag
    14701 gccagcacaa accagactgc gccactgctg tgtgaaccgg acgcacaaaa acgggcgacc
    14761 ggatggcatc agctgtattc cgcagacggt ggtatggcac ccaccggcga tgcaccggat
    14821 gaaaagtgtc tgaccgctgc tcgtgctgcc atcgatgtca ataaccaggc tttccgccag
    14881 ttcggttttg gcggtacccc atgggtgtta tccgataccg gctggcacct gccgtccggc
    14941 atcctgaaag acgccactac cgtcagcctg ttcctgaaag ccacagatac cgaggataat
    15001 cacaatgagc cagcatcacg ttaatcctga cctcatacac cgcacggcct ggggaaaccc
    15061 gctgtggaat gcactgcaca acctgaacat cattggtctg tgtctggccg gcagtatcat
    15121 tacggccctg atatggccac tggcccttcc tgtctgtctg ctgttcaccc tggtcacctc
    15181 cgtgatattc acgctgcaac gctggcgatg ccccctgcgt atgcccatga cgctcagcct
    15241 tgatgaccct tcccaggaca gaaaggtcag gcgaagcctc ttcagcttct ggcccaccct
    15301 gtttcagtat gaagcagatg aaacatcccc cgcacggggg attttttatg tcggttaccg
    15361 ccgtatcaat gatatcggtc gtgagctctg gctgagcatg gatgacctca cccggcatgt
    15421 catgtttttt gccaccaccg gtggcggtaa aacagaaacc acctttgcct ggctgctcaa
    15481 cccgctctgc tggggaagag gctttacctt tgttgacggc aaggcacaga acgataccac
    15541 gaggacaatc tggtatctct cccgacgttt tggccgggag gatgatatcg aagtcatcaa
    15601 cttcatgaac ggcggaaaat cccgcagtga agtcattcag agtggcgata aatcccgccc
    15661 acagtcaaac acctggaacc cgttcgcatt cagcacagaa gccttcaccg ccgaaaccat
    15721 gcagtccatg cttccccaga acgttcaggg gggagaatgg cagtcacggg cgatagccat
    15781 gaacaaagcc ctggtctttg gcacaaaatt ctggtgcgtc cgggaaagaa aaaccatgtc
    15841 cctgcagatg ttacgggagc atatgacgct ggaagggatg gcaaaactct attgccgtgg
    15901 gcttgatgat cagtggccgg aagaagccat cgcaccactg agaaattacc tgcaggacgt
    15961 tccgggattc gatatgtcac tggtgagaac gccgtcagcc tggactgagg agccccgtaa
    16021 acagcatgct tatctctccg gtcagttctc ggaaacattc acaacctttg ccgaaacctt
    16081 cggggacgtc tttgcggcag atgccggcga catcgatatt cgcgacagta tccacagcga
    16141 ccgcattctg attgtgatga tccctgcact ggacacatcg gcccatacca cgtcggcact
    16201 cggtcgtatg ttcatcaccc agcagagcat gatcctcgcc cgtgacctgg gctaccgcct
    16261 ggaaggcaca gacgcacaga ccctggaagt caaaaaatac aaaggccgct tcccctatat
    16321 ctgtatcctt gacgaagtcg gtgcctacta cacggaccgc attgcagtgg aggccaccca
    16381 ggttcgttca ctggagttct ccctgataat gacggcgcag gatcaggagc gaatcgaagg
    16441 gcagacttcc gccaccaaca ccgccacgct gatgcagaac gccggcacca aattcgccgg
    16501 acgcattgtc agtgacgaca aaacagcaag gacggtgaaa aatgccgccg gtgaagaggc
    16561 ccgcgccaga atgggcagcc tgcagcgcca cgatggtgtg atgggtgagt cctgggtgga
    16621 cggtaaccag atcacaatcc agatggaaag caaaatcaac gtgcaggatt tgatcagact
    16681 gaatgccggt gagttcttta ccgttttcca gggggatgtg gtgccgtcag cctctgtgta
    16741 tattccggac agcgagaaaa gttgcgacag tgatccagtg gtcatcaacc gttatatctc
    16801 cgttgaagcc ccccgactgg agcgtctccg ccgtcttgtc cccaggacag tgcagcgcag
    16861 gctccccaca ccggagcatg tcagcagcat tatcggtgtg ctgacagaaa aaacctcccg
    16921 gaaacgtcgt aaaaaacaca cagagcctta ccgaatcatc gataccttcc aggaccagct
    16981 taaaagaaca caaacatcgc tggatctgct tccgcaatat gatacagata tcgaatcccg
    17041 ggcaaacgaa ctgtggaaaa aggctgtgca cactatcaat aacacaacca gggaagaacg
    17101 ccgggtatgt tatatcacac tgaatcgtcc ggaagaggaa cactctggtc ctgaagatat
    17161 accttcagtg aaggcgattc tgaatactct gctccccctg gagatgctgt tacctgtacc
    17221 ggatctaact gcatccccgc cccataaaaa aaatgtcgct cagacaccat caggtggaaa
    17281 acaagagagt cgtaaaaaaa gattctgata acacctgtat ggctgatttt aataacaacc
    17341 tttacgcgat tattaatctc ctccgctcac catagtaagc ggaggattca ggattcaatc
    17401 catcaccaga ggtacaataa atgtgtatta gtagcccgga aacaaactca tggtctgtta
    17461 tttatcgtaa aaattccgga gaagatatca atatcacttc cctgacgttt aaaaatagtc
    17521 ttctggcagc ccggacactg atggtgcccg aaaactatat gatatgcata ctcagaaacg
    17581 gggagagggt cagatgatgg gacagagaaa tacttgcagg ctcaaacaga tggtacaagt
    17641 gttctcccga taatttcgaa atactgggta agctccccat aataaataaa gtcacaacac
    17701 tgataaaatc ataatctact gattttaatc agttaagcta aatcaggatg aaggctttga
    17761 ttatgaatta tacataaaaa caaatatggg cagcacaaaa aatggtgcaa gggttcccca
    17821 cgttcgtaat agattcactt ctatggatat tggtatgaat tcccagatcc tgccagacat
    17881 gctgattaat tttgccctta ttaacatcac agacagaaag aacgagggta tcaataccat
    17941 tgatggtaac tggcaggccg atgaaggacg ccgttaccgg gataatgtaa gaatctattt
    18001 ttgagtatgt caacataacc tgaggcaaag tacctcaggt tccagtgtag taatcagcca
    18061 acctgacagt cttctgttat ccccacagat atgtcgtgga gctaaccgcg catgtcaaag
    18121 ccggattttt gaaaatctct ataaacttcc ggattgttga aggccgggaa tttagcttta
    18181 tgggctgctg actttatcgc ttcgcaatag gctttatcgc cattactggt tgatattttt
    18241 aacgccgtgc catcccggga gaattccata tgcaacctgc attttttcct tttccatttt
    18301 tgcggatcat caagtttagc attaattgca tccctgattc cccgtgcctg ctccccccat
    18361 tcatcctgat catcccaacg tcctgaactg caactaccca gggctgtagt tttatggcaa
    18421 tttgaaggat gtaatggcgc acatcccgcc acaaagctga ctaaaatagc cagtataacg
    18481 attttcttta cccccatata ttgctcctca atccatatag atctcagtac catagctctg
    18541 tcagagcaaa gaaaatctta actaatacaa gttaagattt ttaatatcca ctcctcatcc
    18601 ctacattcca ggtgatggtg gcgtccactc attatcctga actggagcat aagatgcgcc
    18661 atgccccccg gatatgcttt tctgttgcct gatatattcg tggtagtact gttcactgcg
    18721 ctgacgatgt aaatccacat ccgggcggct gattacaaag tgtccatttc cggttattcc
    18781 agctgcatta tgccaggcca ccatttccgc aaatacatca ttaaactgtt gatatgagcc
    18841 ttccggttca aaacccattc ctggctcacg atcaaacaat acaggagcaa tcagattcag
    18901 cttatccacc agttcatttc tgtcctgatg atggtagaac accacacggt ccccataatc
    18961 cacacaaact aactcgccat tcctccgatc acgaaaacgc acactgccat ctttctgcag
    19021 acgggcttcg agaacgccag tgtcttcata aagtggagtt cctcccggtt cacagataat
    19081 cacgcagcgg tcggcattcg ttgttcgtcg cttgtctttg cgacgatcac gataatccca
    19141 gccacgtaac tgtgataatg cagctctgtc tccctgtacc gcctgttgct ccacccactg
    19201 ccggtaggac aaaggatacc acttaccctc cgcaatcagt gacaaccgct cttccttcac
    19261 actctccttc agcctgatca gcgcctgcat acgctgcact tccgcaatat ggtaatgcag
    19321 cttacgcaat tgtggatccc ggaactgcac acggatatac gccttgcgac gacggcagac
    19381 agcatgaatt tcccgcaacc gttcgccgta acgtaaatcg ggtttacgcc agtgttcctt
    19441 ccatgcaaga taacgcgcac gcaaatcttc tctcgcagct gcacgggctt cacggcgttc
    19501 acgacgtagc acaggatcgc gccggaatcc tggctccact tcatcgctgg cagcaagttc
    19561 cggattgtag cgacattcag gcttcacccg ttcaaatata tctgcagcag ccatttcaaa
    19621 tggccctgcc tgaggctcgg tccgggataa cgtcaaatcc gggtggatac tgctggcctt
    19681 cactggtgtg agctcatgat taaacgcatc cacaatcaca agaccatgat gctgtttctg
    19741 caacataagc cctttacgcg caaacatctc atggcacgcc tgccagctga aattcccgcc
    19801 cgcgatcata tcttcaatca acgactcaca ctgaacagtc gtgtaccccc acagactttc
    19861 cttatctgaa aacagttcgc gctttattgc tccctgtgga cctgtcccca tccgcatacg
    19921 ataccattcg taatcactga cagcctgagt accaagatta cgtccccgga atcgttctcc
    19981 gggtataacc tgagtaaaaa tatccttcgg tacaggctgc catccaccat ccagatcctg
    20041 catataatcc agcgtcatta acggccacac actgtctgca cgtactggtg tctgttgttt
    20101 atcaaatcca tcatgcagta ccagatgccc gtgctggatc cccatccata atccagcctt
    20161 tgccagagtt ctgtgcacat ccaggcaact ttccagctca ccatttctcg ccatttccgg
    20221 taagcgttcg gcaaaatgac ggcaggcgta ctgtttcaga ctctccgttt ctgccacttt
    20281 ttccggccgg atatcaacgt ttatggcttc cggatcataa cgaccgggtg tccccacctg
    20341 actgaaaata tctgtcggta caggctgata ttcccccagt ttccacccca gtttttcagc
    20401 tgtccatgac ggtccgaacg aactgagtgc aacaccttcc cgcgctctgt cccagccatc
    20461 cttcaccacc agttcccctt cctgcatcgt gatatacaga ccatcactgg ccagccgttt
    20521 atgcaaagat aaccagtcct gtgctggctc ttcacgcagc ccggcaatcg acatctgagc
    20581 aatatattcc ctgaacgtct gctttttccc gcgtcgccag gcattacggc gctcacgtac
    20641 cagcgctgtt ttgcgcacaa tacggttgcc gggtgcatgc acaaagcagc cattatccgg
    20701 cgcaaatccg tgcttaagtt caagttcacg gcatgcacga ctgagttttt cctggctcca
    20761 ggaaagccag ttaatataac cagtttcagg atgtacacga ttcacggcaa catgcacatg
    20821 cagattatcc gtgtccgtat gaacggcagc cacgtactga tgttttgaga gttcaagaga
    20881 tttcagtgtg tgacgcacac agtcaaaaag ctgctcagga cgcgggcttt catgcgcagg
    20941 ccaggaaaga atgtagtgga aaacggggtc tgtatcatta cgggaaaaaa cagctttgtc
    21001 cgccttgtac tgcatctctt cagcagctgt ttccagtgaa ttgcagttat ggaaacaggt
    21061 tacgccatag aaatttaccc actgactgcc gtctttcatc acatcaatca gggagacaaa
    21121 tttttcgtta cgcaggcgtg ttgcataatc aacaagccgt ctgaaacggt tgcggtgagg
    21181 gacatcactc ttgatttcgc tgtcttctgt gagctcattt tcctgcacat catcccgcac
    21241 agacgtgtac gcaatcaggt cttcaaagga agacctgccg tcacgtcttt tgtgcggaat
    21301 aatggcattc atcagcagtt atctcagcgt cccattaaat ctcttcgcag agcattgaca
    21361 tattcagtaa tggcaacaag aacctctgca tactccttat caccagtgcg ttttccctcc
    21421 acaaacagat gcttctgaag gcggccaaga cgcataatct catcaatcat acggtcgtca
    21481 gtacggcagt taaccacctt atccagtgaa gagcttcgta taaatgttga aaccgtctgt
    21541 ccacttgact ccgctttttc tcttacgatt tcagcctcat cctccgtgaa tctcgccgtg
    21601 aattttacaa ttctcttcct gttctcactg cccgttcggg ttttcttctc actcatggtc
    21661 tgctccttac cggggtgtcg gggcaaagcc ctgaccaggt ggggaatgtc tgagtgcgcg
    21721 tgcgcggtcc gacattccca catcctgtcc cgtttttcgg tccattatag cctgttattt
    21781 ttgtgcatca tagtcttatg cagacggcag aggtagggca cagccctgcc gggagtcagt
    21841 ttttgatgat aaaatcaggc ttcaacatga aacggagaga gagtgccaat ggcttctaaa
    21901 aaattttact cagatgatga catccagctg gcaaaagctg ctttatcgga attgccggac
    21961 ctcacagctc aacggaaaac attgcgtgat tttcttgatg ccattcgtga tgacattatt
    22021 gtcctggtaa ggacaaaggg ttatacactg gcagatgttc gtgacacgct caaaaatgca
    22081 gggtatgaag taggagagaa ggcatttcgt gacattattc gggaagcaga atcgaaaaaa
    22141 acatcccgcc gttcttcctc taaaacagca cctaaaaaga cagattcagc caggaaagat
    22201 ggcatcgata tgaacaacaa ttgatgtgac aatgtcacat cgtattctga gattatgatg
    22261 attacagaga aaaagccccg gcataaatac cagggctttt ctgtttaatc agactcgtgg
    22321 cggcaatgca ccaggataat gccgtgcaat aatatcattc agtttttcaa caaggtcaga
    22381 acgcttaaag acaatgtgta cggttccttt ctggtagcca cgcaccgtaa aatactcccc
    22441 ttctaacagc ttaccgacat tgtctccgtt cccgagaaat tcaccatact gcgcaccgat
    22501 gctgctccgg aagtcaggaa cgttgcgccc ctccagcaga taaaacggac gtaccaggtc
    22561 atccagtgcc tcccgtccgc tgcagtccag cgtcacacgc ccgttactcc agcggtacag
    22621 caggttttca atgatgattt tcttgcccag acggcaggga ttattggttc tgtaatccca
    22681 tgacaatttc cgccagacat caatcagtcc ctgttcaaat gtctgcatct tactggcatt
    22741 cagatggcga aaagttgcca gtacattgtc cagggttatt tcaggacagg tgtcgctcat
    22801 cagctggctg ttccactcat cacgctgaca tgaactcatg aaggtgtaca tccccgtatc
    22861 cgtcatcagg cggtcccaga ttttccggtc ggcaagacgg gtgatattcc ttttcgcttc
    22921 ttccggttta tccatgcgac gaacggagtc agtcacgcag tcttcaaacc cgtagagaaa
    22981 aatgtcacct gacgcatcaa gcagaatcct gcgtgctgtc gccaggcatt ccatcgcctg
    23041 catgaacagt gaaatacctt cgttacgctg cgcgatgatg cggtcgatag ccacagacgg
    23101 aataacatct gtcgtgccgg cagtattcag gtcagcagtg ggtaaagtct gattcatcac
    23161 attgtctcct catcgtcacg actgccgggt cagccaggtc atttcccgga ggagcgaagc
    23221 tccttctgcc gtccgggtct tcggggattt ccccgcagtg gacctttccg acgggggacg
    23281 ctcaccacgt cacggggcag ttgtccgggg cagacgcaaa agtttttgac gcctgcggca
    23341 aaaagttttg tggctggtac atctctcccc tggatgactg aaacgggatg tggtcgtgta
    23401 tgtacccccg gaaagtccgt ctgcggagaa caggccgcag tgcggcggca gacaggcaga
    23461 gacagctgcg ccatcaggcg cagtggcctt acacaccgta aggtgtgcag ggagcgccgc
    23521 atccggcgcg actgaagaaa aacgacggca caacaatggc atgtcgtggt cactggtgca
    23581 ccactcccgc acaggtgaaa acagagaaaa caacacgaca tatacaatca aaaaaagtga
    23641 aatttcttca ctttttcact cttttttatc gcgctgactt ggtgactatg atgataaaat
    23701 aaagtgaagt tttttcactt tttcaaaggt gttttatgac cggctgggaa ctgcgaatct
    23761 ggcgaaaaag catgctctgg tcacgcgaaa aagcggccag agaatttggc gtgacacaga
    23821 gaacatggca cgcctgggaa aatgcggaac aggtggatgt caccgtctgg aggaccactc
    23881 aggcactttc agtccgtgat ctgctgcctc acatgcaggg gatgcgcaag gcagatatca
    23941 tctggcgtct ggaaaatgaa ctgggggaaa cggcagagga tgtgtgagct tgattcacag
    24001 cctggctgaa tgccaggcta ctgctcatca ggaaacagtt ttttcagttc ttcaccggaa
    24061 agtccggttg cctcctgaat ccgttccggc ggccagccca tatcccgaag tcggcaggct
    24121 gcttcccgcg ccacttcaag agcacctttt tcaaaacctt tctgtataag acgttctgca
    24181 agcgtcatgc tgtttgctct gctcctgctg gataaaaagc ccggcatttt gccgggctgt
    24241 gatactctca tcagtgagtg attttcttca gttcgtcatc ggaaagcccg gtagcctgcc
    24301 taatctgctc cggcgtcatc cccattttct gaagctggca cgcaatagcc agggcttttt
    24361 caagtgctcc ttccatgcgt ccttccatgc gtccttccat cctgccttcc tggcgacctt
    24421 tttgctcaag acgttctgcg atagtcatca gtttatcctc atgctgcggc agacgctcag
    24481 ccagcgcccg cataaattcc ggtgatacag agtcccctgc gttaacgatg taatgaatca
    24541 gaacacgcac ctgctcgtct gaaatttcca ccatgacctg cgggagtttg tccagcagct
    24601 ccatcatgtc acgatggcgg atatgcttca tcagcagcgt cagtgcggcc atccggcgat
    24661 ggttcatgat ttcattgtca tcaacgacag tgatatcaac cagcggaaac ggctttgtgt
    24721 atattttagc cgcaagcgcc ggttcctcaa aacagtccag ccagttcatg ctgtacgggt
    24781 acgggctgcg ctcaccatga tagaacagca ccggaatcac cagtggcaat gttttgtgct
    24841 gctccagatg tctctgcatc gccgccacac tgtaacgtaa catgcggaaa ggcatgtaca
    24901 gattttcggt gctctggtgc tcgatgagcg tataaacata gccgtcttta cccccggtgg
    24961 ttttcacact ccagaggata tcgctggcgt actgatgcag gtccggctca acaaaggagg
    25021 cgggttccag cttcagcgtg gacaggtcgc acaactgccg gtactccgcc ggaagatgca
    25081 gttccagaaa atctctggcg acgtcggggt tcgccaggaa tgaccggaac gcggcatcat
    25141 gcggcgtggg cgttgtggtg tttttcttct tactcacggt tcctccatca aatcagcagc
    25201 atcgcagcaa tcatcgtcca aatccccaca accaagagga cagcaattgc gatcaaagcc
    25261 gtgacaacaa tagccacgtt ctgcgtcacg gcgtatttct tctgcaatct cttcagcgga
    25321 aaggtaatca aactcctcac gggccagttc caggaccagc agatgttgtt ctgtcggcaa
    25381 tgtagacaca aaattatcaa tagcagcact caaacgtcgc tgatatagct cataaacaag
    25441 accataacgg tgcattagtt cttcggataa tagttccagt tttgagcgaa cgctgtcagc
    25501 attgaaagtg acttcatcag atgacatctg aaaatctttg tcagtgtcaa aacaagactt
    25561 catttttatc tcctgcccgt ccgggccctg aatgaaagag aacctgtttc ccttctctgt
    25621 ttttttatgg aacaggagga atccggtacc atgcgacata gacgtcatga tgtttttcat
    25681 cttgctcacg gttcctcctg gtaaagtacc tgaacatatc ctgccacctg cacagacaga
    25741 cggcaggggg taatcagtgg cagacccgtt caatttcaag cgcacggagc aggtgccccc
    25801 actcgctgac tttcatggcg gggatacgca cccgcttccg ctcactgcag cgtctggcga
    25861 tatgaccgcc catcgccagg acgtccgggt taatctcatc cggtgccagt tcctggcgtc
    25921 tgacgcgggt atggcggtgc aggggtttat tctcccgcag gcactcttca cgcagacgct
    25981 ttacatattc agccgttgca aagataattg atggcctcac aaatatttct ccttatgttg
    26041 ttgatgactg ccgctcttcc tggccgggaa gcagcggcgg agagaggcag ggtttatcgc
    26101 cctgcttttt ttcattccgg gcagcgacgc agtgccagcg cgagcgcttt catcttccag
    26161 ccttccggcg tgcggtacat caccttccag tagccgtcga tgaacacata atggaagcgc
    26221 acctcttctg tgctttccgt ggcaatagcc tccatgaaag aacggtactc acgcgcctca
    26281 ctgtccatcc ggaaaccact gtcacgtccg taataagccg tcacaccgtc caccgggttc
    26341 tgaagcgtat gcccggcggg tttgtcgcaa cggggaccga ggcaacggat atctccgtgt
    26401 tcaaccaggg cttcggccag ctgaccggtg gcatagtggg tatgcaggat gcgaccgttc
    26461 caggtcggct caccgtgcag gtggcagtaa acggtacgga acaccccgtc gcggcaaaaa
    26521 acggaaatcg tgctgctgac tgtcatgaaa acgtctcctt ctgctgaatc aggtcgccgg
    26581 gccgaccggt tgctcccccg gaggggcgaa agccccttct gccgtccact tcttcggggt
    26641 ttctccccgc agtggacctt tccgatgggg cagcgacagc cacggcacgg ggcagttgtc
    26701 cgggggcaga cgcaaaagtt tttgacgctt gcggcaaaaa cttttgtggc tggtacatct
    26761 cccccctgga cggctgacac gtgttgtggc cgcttcagcc cccggaaagt ccacagcggg
    26821 aaacaggccg caggggtggc ggcggaaggg cagacaactg cgccatcagg cgcagtttcc
    26881 ttacacaccg tcaggtgtgc agggagcgca ggtcacggcg cgacagaggc ggcgcgaccg
    26941 gaacggtcgc tgcaagcctg gtttttcaga catc
//
LOCUS       DAZGVQ010000062        26450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000062 DAZGVQ010000000
VERSION     DAZGVQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.062"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..719)
                     /gene="rfbA"
                     /locus_tag="ACHLAG_002474"
     CDS             complement(<1..719)
                     /gene="rfbA"
                     /locus_tag="ACHLAG_002474"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP0655682.1"
                     /translation="MKGIILAGGSGTRLYPITMGISKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILIITTPEDQFSFIRLLGDGKHLGVSISYAVQPRPDGLAQAFTIGKDFIGADSV
                     CLVLGDNIFFGQGFTPKLKTACERKLGATIFGYQVRDPGRFGVVEFDSDYRVLSIEEK
                     PLKPKSNWAVTGLYFYDNDVVEIAKSITPSQRGELEITSINEVYLKNNKLTVELLGRG
                     FAWLDTGTHDSLIEAGSFVETV"
     gene            complement(716..1792)
                     /gene="rfbB"
                     /locus_tag="ACHLAG_002475"
     CDS             complement(716..1792)
                     /gene="rfbB"
                     /locus_tag="ACHLAG_002475"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP0655683.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYIFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTINWY
                     LNNNDWCRRVQDGSYQRERLGVIR"
     gene            complement(2165..3058)
                     /gene="galF"
                     /locus_tag="ACHLAG_002476"
     CDS             complement(2165..3058)
                     /gene="galF"
                     /locus_tag="ACHLAG_002476"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFP0655684.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGANFRKGIEKLLSE"
     gene            complement(3233..4627)
                     /gene="wcaM"
                     /locus_tag="ACHLAG_002477"
     CDS             complement(3233..4627)
                     /gene="wcaM"
                     /locus_tag="ACHLAG_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFP0655685.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPDQQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEKGGSMHNITLDVRGSDCTIKGLTMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDRDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNALSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(4638..5858)
                     /gene="wcaL"
                     /locus_tag="ACHLAG_002478"
     CDS             complement(4638..5858)
                     /gene="wcaL"
                     /locus_tag="ACHLAG_002478"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFP0655686.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPAGKVAKLRHRASQTLRGIYRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSRDV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(5855..7135)
                     /gene="wcaK"
                     /locus_tag="ACHLAG_002479"
     CDS             complement(5855..7135)
                     /gene="wcaK"
                     /locus_tag="ACHLAG_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFP0655687.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(7314..8792)
                     /gene="wzxC"
                     /locus_tag="ACHLAG_002480"
     CDS             complement(7314..8792)
                     /gene="wzxC"
                     /locus_tag="ACHLAG_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFP0655688.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWISIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIATGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(8794..10188)
                     /gene="wcaJ"
                     /locus_tag="ACHLAG_002481"
     CDS             complement(8794..10188)
                     /gene="wcaJ"
                     /locus_tag="ACHLAG_002481"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFP0655689.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLIKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(10243..11613)
                     /gene="cpsG"
                     /locus_tag="ACHLAG_002482"
                     /gene_synonym="manB"
     CDS             complement(10243..11613)
                     /gene="cpsG"
                     /locus_tag="ACHLAG_002482"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFP0655690.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     SYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAHPIEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(11806..13242)
                     /gene="cpsB"
                     /locus_tag="ACHLAG_002483"
     CDS             complement(11806..13242)
                     /gene="cpsB"
                     /locus_tag="ACHLAG_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFP0655691.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(13245..14468)
                     /gene="wcaI"
                     /locus_tag="ACHLAG_002484"
     CDS             complement(13245..14468)
                     /gene="wcaI"
                     /locus_tag="ACHLAG_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFP0655692.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNIIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKYNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(14465..14944)
                     /gene="gmm"
                     /locus_tag="ACHLAG_002485"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(14465..14944)
                     /gene="gmm"
                     /locus_tag="ACHLAG_002485"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFP0655693.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(14944..15912)
                     /gene="fcl"
                     /locus_tag="ACHLAG_002486"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(14944..15912)
                     /gene="fcl"
                     /locus_tag="ACHLAG_002486"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFP0655694.1"
                     /translation="MSRQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(15915..17036)
                     /gene="gmd"
                     /locus_tag="ACHLAG_002487"
     CDS             complement(15915..17036)
                     /gene="gmd"
                     /locus_tag="ACHLAG_002487"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFP0655695.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(17062..17610)
                     /gene="wcaF"
                     /locus_tag="ACHLAG_002488"
     CDS             complement(17062..17610)
                     /gene="wcaF"
                     /locus_tag="ACHLAG_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFP0655696.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDEVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(17626..18372)
                     /gene="wcaE"
                     /locus_tag="ACHLAG_002489"
     CDS             complement(17626..18372)
                     /gene="wcaE"
                     /locus_tag="ACHLAG_002489"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFP0655697.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQNAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(18383..19600)
                     /gene="wcaD"
                     /locus_tag="ACHLAG_002490"
     CDS             complement(18383..19600)
                     /gene="wcaD"
                     /locus_tag="ACHLAG_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFP0655698.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWVFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(19575..20792)
                     /gene="wcaC"
                     /locus_tag="ACHLAG_002491"
     CDS             complement(19575..20792)
                     /gene="wcaC"
                     /locus_tag="ACHLAG_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFP0655699.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIVLFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(20789..21277)
                     /gene="wcaB"
                     /locus_tag="ACHLAG_002492"
     CDS             complement(20789..21277)
                     /gene="wcaB"
                     /locus_tag="ACHLAG_002492"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFP0655700.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNIWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(21280..22119)
                     /gene="wcaA"
                     /locus_tag="ACHLAG_002493"
     CDS             complement(21280..22119)
                     /gene="wcaA"
                     /locus_tag="ACHLAG_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFP0655701.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(22297..24459)
                     /gene="wzc"
                     /locus_tag="ACHLAG_002494"
     CDS             complement(22297..24459)
                     /gene="wzc"
                     /locus_tag="ACHLAG_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFP0655702.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQILKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(24462..24905)
                     /gene="wzb"
                     /locus_tag="ACHLAG_002495"
     CDS             complement(24462..24905)
                     /gene="wzb"
                     /locus_tag="ACHLAG_002495"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFP0655703.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWEQALNAEQV"
     gene            complement(24911..26050)
                     /gene="wza"
                     /locus_tag="ACHLAG_002496"
     CDS             complement(24911..26050)
                     /gene="wza"
                     /locus_tag="ACHLAG_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFP0655704.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
BASE COUNT         6298 a         7112 c         6454 g         6586 t
ORIGIN      
        1 acagtttcaa cgaaacttcc cgcctcaatt aggctatcgt gcgtgcctgt atctaaccat
       61 gcgaaaccac gcccaagcag ttcgaccgtc aacttgttgt tttttagata aacttcattt
      121 atcgaagtta tttctagctc acctcgttga gaaggagtaa ttgatttggc aatttcaaca
      181 acatcattat cataaaaata aagtccagtt acagcccagt ttgattttgg ttttaacggt
      241 ttttcttcaa tacttaatac cctataatct gagtcaaact ctacaactcc aaaccgtcct
      301 ggatctctta cctgatagcc aaaaattgtg gctcctaatt tcctttcaca tgcagttttt
      361 aattttggtg taaacccttg accaaaaaaa atattatcac ctaatacaag gcaaactgaa
      421 tcagcaccta taaaatcttt tccaattgtg aaagcttgcg ctaaaccatc tggccttggt
      481 tgcactgcat aactaatgct gacacctaga tgttttccgt caccaagcaa ccgtataaaa
      541 ctaaattggt cttcgggcgt tgtaataata agtatatctc tgatccctgc caacatcaga
      601 acagacaatg gataatatat cattggcttg tcataaacag gcaataattg ctttgaaatc
      661 cccattgtaa ttggataaag ccttgttcca gaaccaccag ctaaaataat tcctttcatc
      721 taattacccc caaacgttca cgttgataag agccatcttg aactcgccgg caccaatcat
      781 tattattcaa ataccagtta attgttttac gaatcccgct ctcaaacgtt tcctgtggtt
      841 tccatcccaa ttcgcggcta atcttctcag catcaatcgc ataacggcga tcgtgtcccg
      901 gacgatcggc aacataagtg atttgctcac gataagattt ctctttcggt acaatctcat
      961 ccaacaaatc acaaatagtg agcactacat cgatgttttt cttttcgttg tgtccaccaa
     1021 tgttataagt ttcacccgct ttaccttcgg ttacgacggt atataacgca cgtgcatgat
     1081 cttcaacata cagccagtcg cgaatttgat cccctttgcc ataaataggt aatgccttac
     1141 cttccagagc attaagaatt actagtggaa tcaatttttc cggaaagtga taaggaccgt
     1201 agttattcga acaattagtc acaatggtcg gtaaaccata ggtacgtttc cacgcacgga
     1261 ctaaatgatc gctggatgct ttggatgcgg aataagggct gcttggcgcg taagctgttg
     1321 tctcagtaaa taagggtaat tcttctgtat tatttacctc gtcaggatga ggcaaatcac
     1381 catatacttc gtcagtagaa atatgatgaa aacggaagct atttttcttg tcgctatcaa
     1441 gagcagacca gtaattgcga gcggcttcca aaaggacata agtaccaaca atattggttt
     1501 caataaatgc cgcagggcct gtaattgaac gatccacatg gctttcagca gccaggtgca
     1561 tcactgcatc cggctgatgc tgagcaaaaa tccgtgccat tgcagcagca tcgcaaatat
     1621 ccgcatgttc aaaaatatag cgttcagaat cagaaacatc agcaagtgat tccaggtttc
     1681 cggcgtacgt taatttatcg acattaacaa cactatcctg cgtattattt ataatgtgac
     1741 gaactacagc agaaccaata aatcctgcgc caccagtaac aagtattttc acttaattta
     1801 ttccatatta catcagagca tgctgtgaaa taagcggctc tttgtttgat taatagaggt
     1861 attaatgcac gctaccgccc ctggctttac agctaccaga gcactgcatg catgtctacg
     1921 atgtgacgag cgttacccac tcgcgctaaa cccgaaaaat tcaaacgcta attgtcttac
     1981 caatccgctc tggaaacaag gaaaatcctg gaaaactttg actaaaatcc tattgctaac
     2041 tcgttgttat tctgattgtt tatataaaac aacggcagga atattcgcag caaattactt
     2101 tcgccacgaa tcttcactgc cgttataatt ttcttatcaa ccgttacatc cggtcagatt
     2161 ttcattattc gcttaacagc ttctcaatac ctttacggaa attcgcccct tctttcaggt
     2221 tgcgtagtcc atacttcaca aatgcctgca tataacccat ttttttaccg cagtcgtagc
     2281 tgtctccagt catcagcatg gcatcaacgg actgtttttt cgccagttca gcgatggcat
     2341 cagtcagctg aatacgtccc catgcaccag gctgagtgcg ttcaagttcc ggccaaatat
     2401 cggcagaaag cacataacgg ccaacggcca tgatgtctga gtccagcgtc tgcggctgat
     2461 ccggtttttc gataaattcg acgatgcggc ttactttacc ttcgcggtcc agcggctctt
     2521 tggtctgaat gacggagtat tcagagaggt cacccggcat acgttttgcc agcacctggc
     2581 tgcgtcctgt ttcattgaag cgcgcaatca tggcagcaag gttgtagcgc agcgggtcgg
     2641 cgctggcgtc gtcgatcaca acgtctggca gcaccacgac aaatgggttg tcaccaatgg
     2701 cgggtcgtgc acataaaatg gagtggccca aacctaaagg ttcgccctga cgcacgttca
     2761 taatggtcac acccggcgga cagatggact gcacttccgc cagcagctga cgcttcacgc
     2821 gctgttcaag gagagattct aattcataag aggtgtcgaa gtggttttcg accgcgttct
     2881 tggacgcgtg agttaccagg aggatttctt tgatccctgc agccacaatc tcgtcaacaa
     2941 tgtactgaat cattggcttg tcgacgatcg gtagcatctc tttgggaatc gccttagtgg
     3001 caggcaacat atgcatccca agacccgcta caggaataac tgcttttaaa ttcgtcatta
     3061 tttcatccac ctgtaaaatg gttgctgaat tatagcttgt tcgatttttt tcgccagcat
     3121 caattaccct gaattgatta ctgaattact tgtgatgtta cgccgcttcg ttgtggattg
     3181 cagtagcatt gttcctaagt atggctccat ttttccagga atggtcgcaa atctactccc
     3241 tccgttctgg caatctaaag ttaatctttt ccacattaac aatatggtga ttaatcctgt
     3301 cgatatcgac ggagctttgc cctctttcat tcaccgcatg aacattcgca agagatagca
     3361 gtgtttcttt tttcgccata aagacgccgc gaacgtcttt gcgcatgtca aagttcatgc
     3421 tcaatgctgg tccaactgag gattcctgca tcaccttgat atttcgcata aaaagatgtt
     3481 gcggtttgtt gtgtaactcc agcgaggcac gcttcatctc aatgttagtc agcgccacaa
     3541 aggagagagc attcccggcg gagatttgaa tgccgcgcaa tttgtaagca aggtgggtgt
     3601 tatccagttg aatattattc actcggaaat tttgcggtat cgagagatat ttgcctttaa
     3661 ttaccccata gccgattaac atcccggcgc tattaatcat ttcaatatta tcaatcacga
     3721 aattgtcaca accgtaaata gcaactgtcg cgttatcaat gcctgctttc ttactgaaat
     3781 ccggcgtgat attgcgggct ttgatattac gaataacaaa atgtttacca ttttcaacat
     3841 ggatcaactg ccgacaatcc gatcccgtga tattcgccac gacaaagttt ttcactgcct
     3901 ggtcttccgg gtagttgtta tcgtaggtac ttcccgcaag gcctatgcca atgccccagt
     3961 tgattttgcc attagtacag ttgatgcgct cgatgacatg gtcggagatc aaaatatcac
     4021 ggtcgttaat cgccacgttc cattcaatgg catcgccctg taagtcgctg aacttacaat
     4081 tggtgatgtt ggcaccgatt atctggttat gaaatccctg gcgtaagatg gcgtaattag
     4141 cgtggctaac ggtcaggtta tcgatggtca ggttacgcat gacccgtttg tttttgccgc
     4201 cgatatagat ctgcgtcacc gggccaaagc cgctcatcgt cagccctttg atggtgcagt
     4261 cagaaccacg cacatccagg gtgatgttat gcatactgcc gcccttctcc cctgtcacct
     4321 ggctgccgtc ctgtaagaca aatcgccctc tgccgttgcc tcgcaggctt ccgaggatgt
     4381 gtaacgtttt gccaggagga atgaagatgc cggtgttgat attgtcacaa acgaatccgg
     4441 caggcacgac gaccgtttgc ccttcgctga aggcttgttt aaatgaggcg atccagtcgt
     4501 gtgggttgta gtcgttaatg ttaacgctct gttggtcggg aagtgcgcga gcgaaagggg
     4561 tatggaggaa ggcaagcgcc gagcttgccg tcaggaacgt gcgtcgggag agttttttaa
     4621 atggcatgcg ttctcctcta taaagcctgc agcaagctgg cgagttctcg attgatcacc
     4681 tgctggttaa aatcgtgttc gactttttcg cgcgcacgtt tgacaaccgg agccagttcg
     4741 tcagtgtcca gttggctaaa tgccgccaga cgttgtgcca gtgcgcgagc atcgttctca
     4801 ggcaccagcc agccggattt atcggcctcc accagttccg gtattccgct atgcagagta
     4861 gaaactactg gaatgccgac cgccatcgct tccattagcg ccactggaat gccttccata
     4921 tcaccatccg cgcccgttac cgatggcaac aggaagacat ccgcgtcgtc gagcatcgct
     4981 ttcacttcat ggctcggttt aaagcccggc atctctacca catcttccag ttgatattgt
     5041 tcgatgaggg tgcgcaggcg tcgttcccac gggccaatgc cgagaatgcg atagcgaaat
     5101 gccacgccct gctctttcaa ctgacggcag gcttcaatcg ccacatgcag accttttttc
     5161 tcggttaagc gtgcgacgga aatgatttcc agcggcgttg cgggtgcttt caccggacgc
     5221 gggctaaagc gcgtcatgtc cacgcccata cgcgatacgg cgattttttc cctcggacag
     5281 cccatttttt gcagccttcc ggcccacaga tcgcttatcg gtaacatcag gtcgccacgg
     5341 cgaaacagtt gttgatattc gggagtgtag tggttgagaa cgtcccgact ggagatatca
     5401 ataccgtgga agatagtggc aattttgccg cgaataacac ccagttcgcg tagttttgct
     5461 gcggttaccc ccgccgggcc aaaatgagcg ataaagacat cggcatgaaa cggtgttgcg
     5521 acctggccac aaatggcaga caaaatcagg ttccgcgact cggcaccata gcgtttgagg
     5581 ttgagcgcct gccaggtatt tttacgataa atgccgcgta aggtctggct ggcgcggtgg
     5641 cgcaatttcg ctactttgcc cgcaggttcg tcctgtaacc aacgggttct ggcggcaagg
     5701 ttgtatttcg tccatgccgc gtgggtgttt tgtgtgtcgc ctttttgcag cgcgacaatc
     5761 tccacctcaa atcccatatc aataaacgca gtgatttggt tgaggacgaa ggtttccgac
     5821 gacagcggga atttcagtaa aaagaagccg accttcattt cacctccccg atgcgctcaa
     5881 gcacagactg taccatctgc attcctgtct gacgctcacg actgacggct tcgctaagtc
     5941 gtgcgttcag cgccggaagc tggcctaagg tatccgcaac catcgcttgc aggctgccgt
     6001 ctaataaatg acggatatca attgccatct ctggcagtcc caactgttgc ataatcccgg
     6061 cggatttatg ttcgtagttg atggcaattg ccggagtggc aaaattcatc gagataatgg
     6121 cagagtgcag gcgcgtaccg acggtgagtt cacaggcacc gagaattttg cccatttcca
     6181 gatcgttaag ttcatccatc actacgtgat aacgggcagg atcactgatg tgctggcgca
     6241 ggttgagcgc caccatgcgg tcgtctttgt tatagctgtc gatacccgta caggtggaga
     6301 gcgcaataac ctgatagcct tcgtcgagaa tgcgattgac caccccggca aaggcttttt
     6361 catacgcttg ttgagtggtg ccgagacgtt tgtcgaacgg tgccagttcg cgcagggtaa
     6421 tggccaccgt tttctgttgt gcggcaacgt ccagccagtg ttgaacggca tagctggcgg
     6481 tgaaatcttc ggtgtggtga tcgaccagcc acgcggtatc gacgccatgt tccacttttg
     6541 cggtggtgat attgctgcgt ttcatcagat caaggctgac cgattcgcgc aggatcagcg
     6601 cgtcgcagtg accaaaaacg tagttcgcca gttggttaaa ttgctcatcc tggaacgggc
     6661 caacactgtg accaatcata aacagcggct ttttcgccat gaaagtacaa agtgcatgtt
     6721 caaactgcgg cacgccatag agatcgacaa aaaacgatcc gccgacctga ataatggcgt
     6781 cgtaacctga cagcaggcgc acgaagtcgg tgaatccctg ggcgatggcg atattacgca
     6841 gcttgccagt gtcggtgacg cgtgagagca atacctggtg ttgatagcgg cgacggagga
     6901 cttttttaac gcgcccgaca acgcccgccg cgctgttgtg ttgtttcatt tgcaggaaca
     6961 gcggatcgcc cattaccggg cggttgagca gccaggaaga actgaccgga tagcggctca
     7021 tcacgtccac ttcggcttgt ggattgagga tgttgatggc atcaagtaag ccgcgcagga
     7081 tggcgctgtc gccacgattg ccgcaagtgt ggttgcccag aataagtaat ttcatttttt
     7141 cctcataaat ttgatgccag gtgaggctgc gtttgttttg ttcgttgccg ctttttgcct
     7201 gatgcgacgc tgacgcgtct tatcaggcct acaagacccg agcctagaac cgtaggccgg
     7261 ataaggcgtt tacgccgcat ccggcaaccg ttgccggaac cgaaaacaac aactcacccc
     7321 gcccgtaaaa gcattttcat tttttcgctg cgacaaaact gacgctttac ttccaccacc
     7381 agcggatggc gcgacagcac aatcatcacc acaaacgcca gcacacccgt ggctatttgc
     7441 accgccagca gcatccccag cgccagttgc cctttcagca caatgcccag cgcataactg
     7501 accaccagcg tcggcagcga gagataaaac ggcagccata aactcaggat gtactggcga
     7561 taacttgaac cgagtactgg tttaatcatc acgaaataac tcagaatggt gttgataatc
     7621 tgcaccagca ggaaaccgag cgtgacgccg atcgcgcccg ccatctgccc accaataaca
     7681 atcgccggaa taaacagaaa tgttttgaat acgttgaatt taaagctgat atcgacccgc
     7741 gctttcgcca tcagcagcga accaatcggg ttacccactg agcgcagcag acccacaata
     7801 cacagcaatt gcagcaccgg aataatgctg atccacttct caccaaagac cagcggtaca
     7861 aaattattcg acaccaccat cagcccgagc agcgccggaa agttgataat ccccaccacc
     7921 gacagcagct tgtagaagtt gacgcgcagc ttttcggtgt cgtcctgaat tttggcaaac
     7981 gccggaaaca acacgcgggt gatgatgggg ttcagcttca tcggcggcac aacggccacg
     8041 ttgtacgcca ggttgtatcc ccctgctacg cccgcgccga gaatacgcgc cagcaccagc
     8101 gttgaaaggt tggtgttgag atagttgatg atgctgtccg ccgtcagcca ggcaccaaaa
     8161 cgtaagttcg gtgccacaga cgccagcgag aagtgcagac cggggcaata aattttgcgg
     8221 ccaaagtagc caaacagcag cgttcttacc gcactattga ccagataacc gaggatcgcg
     8281 gtcatcgcca gcggccaaaa atgggcgcta accactgtaa aagtgaagcc cgccagcacc
     8341 gcgctggttt cgatcatgcc gattttgttg aactccagtt ccttttgcat caacgcgcgg
     8401 aactgctgcc cgtgggggat caccacaaac gccagcgaca atgttttaat caacggtgcc
     8461 aggtccgggt tattcagcac gtcaccaatg gcatcactca acaaaaacac cgccacgcac
     8521 accacaagcc ccagcccgac gttcagccag tacagcgtgg tgagttcaag gtggctgatt
     8581 tcttttcgct gaataatcga gttagcgata ccgaagtcag aaagcgtatc tgccagcgcg
     8641 atgatcacca gcgacacggt aagcaaaccg aactggtggt tgtcaataat ccgcgccagc
     8701 acggtcatct gtaccagccc gaggccgatg atgatcaccg tagcaatcgc cgaccacttc
     8761 gcgccgctga tggttttttc acgtaagctc atatcaatat gccgctttgt taacgaaacc
     8821 tttgaatacc gtcaggaaaa cgattttaat atcgaaccag acgctccatt cgcggatgta
     8881 ctcaaggtcg aactcgacgc gtttttccat tttctccagc gtgtcggttt cgccacgcca
     8941 gccattaatc tgcgcccagc cggtaatgcc cggtttcact ttatggcgca gcatgtagcc
     9001 ttcaatgagc tgtcgatact gttcgttatg cgccaccgcg tgcggacgtg gaccgacaat
     9061 cgacatcccg ccggtcagca cattgataaa ctgcggcagt tcatccagcg aggtgcggcg
     9121 cagaaagttc cccactttgg tgacgcgcgg atcgttctgc gtcgcctggg tcaccacttt
     9181 gtcgttctcc atcactttca tcgaacggaa cttccacact ttgatcggct tgccatccat
     9241 gccatagcga gtctggcgga aaataaccgg ccctggtgaa ctgagtttca ccgccagcgc
     9301 aatacagcac agcaccgggg agatcagcag caagatcagc gtcgccagca caatgtcttc
     9361 cgcacgtttt atcaggcggt taaccccgga aagcggcgta tcgtacagcg gcaccaccgg
     9421 aacgccgttc atctcttcga gacgtgaatg gagaatgttg aaggtaaaaa catcggggat
     9481 cagcagcacc gaacaggtgg tgtccgccaa ttgatggacc agttttttca ctcgcgcgcc
     9541 gtcgcacatt tgcatcgcga tatagacgtt atgaatctta cccgctttag catcctcgac
     9601 cagctgttgc aggttacccg cccagtcgtt agaaacgccg cccggtttcg ggtcgtggta
     9661 aacgcccacc acttcaaacc ctaaccacgg ctgattacgg aagctctcca tcagcatttg
     9721 tccggcggct aaatcccccg ccaccgcgac catgcgcttg ttatagccat gattacgcag
     9781 ccagcccgcc ccaatgcgaa tacatgaacg gcaaaccacc agtccgatac tggtcagccc
     9841 ataccacgcc agccagattt tcagttgcgt gtcgaaatca ttgttgaacg ccaccagtcc
     9901 ggcactgaaa atcacgctta aggtccagtt ttgtagcaat agggcaaatt ctgtcgctgc
     9961 ccgaacaccg cgccatgagc gataaaaatc ggtgatgcca cccagcatct ggaacaccac
    10021 cagcgtaatc agcgccacca acaggtgcat gtagaggaat gacagtccgc tgacttcgca
    10081 aaccagccat agcccggcaa acatgatggt gatatctgaa aagcgttgca ccatagagat
    10141 taacgatgca ttggttttcg ctcgctcgcg cttttttaga tttgtcatcg ttgttcctgt
    10201 tattagcccc ttacccgcag tggggtaagg gaagatccga cattactcat tcagcaacgt
    10261 cagcagagtt cgcgttcgcg cttccatcag cggcacatca ccgcgcgatt ccacattcaa
    10321 ccgcaccacc ggttcggtgt tggaggagcg caggttaaag cgccagtcgg caaaggtcat
    10381 gctgatgcca tcggtgcgat ccaccgccag cgcctcacgg ctaaaatgct gttccacgcg
    10441 gttaatcgcc tcaatggggt gcgccagttt gctgttgatc tcaccgcttg ccggaaacgc
    10501 agccatccgg tcgcgcacca gttcgcccag cgtttttcct ttcaggcaca ccagttcggc
    10561 gaccagcagc cacgggatca tgccgctgtc gcagtaagcg aaatcacgga agtaatggtg
    10621 ggcgctcatt tcgccaccgt agatggcgtc ttccttgcgc atacgttctt taataaaggc
    10681 gtgtccggtt ttcgacatca ccggcgtgcc gccagcagca gttaccacat caacggtgtt
    10741 ccaggagaga cgtggatcgt ggatgatctt cgcgccggga tttttttcga ggaatgcttc
    10801 tgccaacagg ccgacaatgt agtagccctc aataaactgc cctttttcgt caaacaggaa
    10861 gcagcgatca aaatcaccgt caaaggcaat gcccatatcc gcgccgtgtt tgatgaccgc
    10921 attgcgggtg tcgtcgcggc attctggcag tagcgggtta ggaataccgt tggggaaatt
    10981 gccgtccggc gtgttgtgta ctttgattaa ttccaccggt gcgccgaggg ctttaaatcg
    11041 ggcttcaatg gcgtccacca ccggacccgc cgcgccgttc ccggagttaa tcaccagctt
    11101 gagcggcgtg aggtttttga cgttgatata actgaacagg tgatcaacgt aagcgtcacg
    11161 caggttgatt tgctgatagc gaccgcgttt ggtttcatcg acgggaggaa agtcgttggc
    11221 ttctgccaga cgctggacgt cgcgcagtcc ggtatcaccg ctgatcgggc gagccccttc
    11281 gcgcaccagt ttcatgccgt tgtaatccat cggattatgg ctggcggtaa cttcaatgcc
    11341 gccatccaca ccgagatgga acgtggcgaa atagatctct tcggtgccgg acatgccaat
    11401 atccagcaca tcgacgcccg catcctgcaa accttttgcc agcgccagtt ttaaggtttc
    11461 gctggtgagg cggacatcac cgcctaacac aatggttttc ggtttgagaa attcgccata
    11521 ggcacgccca atacgccagg caatatcttc attcagttct tcgcctaatt tcccgcgaat
    11581 atcataggct ttaaagcagg ttaatttttt catatcgtta ccctttttca ggcaatagtt
    11641 ggccctgacc gaaacggcca attttgtttt tgtcgttatt tacaccgcgg tttcgcattc
    11701 attgcctgat gcgacgctgc cgcgtcttat caggcctaca aaaccgaacc gtaggtcgga
    11761 taaggcgttc acgccgcatc cgacaaacag cgcctgatgc gacgtttaca cccgtccgta
    11821 gcgatccgcg aaacgcacca catcatcctc ttcgagataa gagccggagc gtacttcaat
    11881 taaatcgagc ggaattttcc ccgggttttc caggcagtgc gtcgccccca gcggaatata
    11941 aatggactcg ttttcaccaa gcagtttgat atcaccgtca atggtgactt ttgccgttcc
    12001 cgcaaccacc acccagtgtt ccgcgcggtg atggtgcatc tgtaccgaca agccttcgcc
    12061 cggtttcacg gtgatgcgtt tcacctggta gcggtcgccc gcgtcgatag agtcatattt
    12121 gccccacgga cgatacactt cgcgatgcac ccgatgctca tggcgaccat cggctttgat
    12181 ctgctcgacc acttttttca catcctgtac cgcgttacgg tcggcaatca gcaccgcatc
    12241 tttggtctgc accactacca gatctttcac cccgacggtg gtgaccaggc cagattcggc
    12301 atacacatag ctgttttcag ttttgtgatt aatcacatcg ccgtggcaaa cgttgccctc
    12361 ggcggtgtgg gcgctgatct cccataatga agaccaggaa ccaacatcgc tccagcccgc
    12421 atccatcggc accacaacag catctgccgt acgttccatg accgcgtaat ccaccgactc
    12481 ttccgggcag gcgagaaacg cttcttcatc cacacgaata aaatcaagat ccggatcgac
    12541 ggcgctcatc gctttttcac aggcatcgag aatatccggg cgatattttt tcagttcttc
    12601 gagatagcgt ccggcgcgga acaggaacat gccgctgttc cagtaatatt cgccgcttgc
    12661 cacataggcc tgggcggttt ccagattcgg tttttcgaca aactgcgcca cttcaaaggc
    12721 caccgtatcc tgctcacccg cagacacttc accgcgacga atatagccat aaccggtttc
    12781 tggtagatcc ggcacaatgc caaaggtcac cagcttgccc gcttcggcat atggcatggc
    12841 attacgcacg gcggcacgga acgcgtcttc atcggcaatt acatgatccg ccgccagcac
    12901 cagcattaac gggtcgttct ccgggctatg acgttttgcc gccagcgccg ccagcgcaat
    12961 ggccggtgca gtgttacgcc ctgcaggttc gagaataatg ttctcggtaa gtttgttcag
    13021 ttgacgcagc tgttccgcga caataaagcg gtgctgctca ttgcaaatca ccaccgggct
    13081 ttcgcactcc acgccgttca ggcggcagat ggtggtttgc agcatggtga gatcgccttt
    13141 caggcataaa aactgcttgg ggtaaagtac gcgggaaagc ggccataagc ggctaccgga
    13201 gccacctgcc atcacaactg gatagagttt cgactgcgcc ataattatcc ccgaatatca
    13261 tttataaatt gacgtaacac gttctcttta tcgagcgtgc gttcggcata ttcacgtgcc
    13321 accgtgttgt acttgggcag caggagcgcc tgacgaatcc ccgccaccag cgcctcgacc
    13381 gattccggtt ccacgcaaac cgcaatgccc ggaaaggttt cgcaaagctg tcccagttct
    13441 gtgtgggcct ctgcggtaat caccgcgtta ccgcctactg ccagaatatt ggtcagtttc
    13501 gacggcaata cggcatctgc cgcgccgcgt ttttgcacca ccagatggca atcgcccatc
    13561 ttcagcagtg cgggtaaagc gtcatacgat tgcagcggga aaaattgcat gttgcgcagt
    13621 ccacgctgct gcgccatttt ttccagccgc gctttgccgc cgccctgccc gacaatggca
    13681 aaaatcagcg gttcatcgcg caggcgatcg gcagcttcaa taacgttttc cagtccctgc
    13741 ttttcaccaa tattgccgga gtaaagaatg atttttttgt tatccggcag gccaagctgg
    13801 ttacgaaggg catcaacatc ggcgtctgca acatgctgaa aacgggcgat ttccgaccag
    13861 ttggggaaga aaatgatgtt atccgccgcc acgccttttt cgatggcttt attcatcatc
    13921 gaacgcgaaa tcgtggagac gttatcgacg ttatgcagtc cgctacgttc gaacgccgtt
    13981 gccagctgtg ccactttgcc gcctttgcct tttccggcaa ggcccagccc cagcatggcg
    14041 tccacttcgt aatcctgaat atgcagcacg gtacgcgcac cagagagttt cgccagcagg
    14101 cgcattcccg gcgtgcaaaa cagcgttggc accacgccga taatgcgatc cggcttccag
    14161 cgacgttgcg ccatcaacgg gaaaaaactg ctgacggcaa aactgccgag atgcaacagg
    14221 cgtttcaggg tgctcggctg ttttggcacg tacagcgggc agcgccacac cgtggcggcc
    14281 ccctcttctc gtttgtagcg ccaggcggaa tagttctcgc ccacctgcca ctgcgggtag
    14341 taaggcggtg cggtaataac ccgcacctca tgaccttgcg ccgccagcca ttccaccatt
    14401 tctccggtgt atttgccgat gccggttaac tccggcgagt agttaatgcc gtagaccagt
    14461 attttcataa tccgggtact ccggcacgct tctcagcgag aaaataggcg cggctgttag
    14521 cgtgaacatt gtcactggcg agcagtgcgt ccggcgtcag ccagcggtaa tcgtcatgct
    14581 gctcatccgg cagtaacagt tcttcttccg ctactctgaa gcgaaaaccg agcaccacat
    14641 agtgagtggt gaaatccgtg ccggagaagt tatcgtcata aaagtgctgc cagacaccgt
    14701 aaaactggcc tgctgttatc ggcagacgca gccccagttc cgccatcgtc agccgctcaa
    14761 atgcggcttc cagcgtttcg tctttctgca cgcgccctcc cggcacaaac cagtaaccct
    14821 gcgccgggcg gttggttctt ttgccaagca gaaactcgcc gcgactgttc tcgacaataa
    14881 agtcgagaga gacaagcggt gtggagcgca ctacagtggc aaagtcttcc tgacgtaaaa
    14941 acatcattcc ccccgaaagc ggtcttgatt ctcaaggaac cactggtaag tgctggcaag
    15001 ccccgcttcc agtgagattt cgtgatacca gccaagctga tgcaggcgcg tcacatccag
    15061 cagtttgcgc ggcgtaccat ccggtttgct ggcatcaaaa accacccgac ctttgtaacc
    15121 caccactttg gcgatggttt gcgccagctc gcggatagtg cagtcaacgc ccgtaccaac
    15181 gttaatgtgc gacagcatcg gctgggtgtt ctccagccag acttcatgcg ccagctccat
    15241 gacatgaatg ctcgccgccg ccatgtcatc gacgtgcagg aattcacgca tcggtgtacc
    15301 gctgccccat accaccacgt ccggcgcatt ctgtgccgtc gcctcgtgga agcgacgcag
    15361 caatgctggg atcacatgcg agttactcgg gtggaagttg tcatgcgggc catacaggtt
    15421 ggtcggcatg actgagcggt aatcgcgtcc gtactggcgg ttgtatgatt cgcacagttt
    15481 gatcccggcg attttggcaa tggcgtaagg ctcgttggtc ggctccagcg tgccctgcaa
    15541 taactcgctt tctgccatcg gctgttttgc cagtttcggg tagatacagg acgatccaag
    15601 aaacagcagt ttgttcacgt cgttctgatg cgcggcgtga atgatgttgc tctcaatcat
    15661 catgttctgg tagatgaaat ccgccggata ggtgttgttg gcaacaatgc cgcccacttt
    15721 cgccgccgcc agatagacct ggtcaatgcg ttcgctggca aagaaatcat gcaccgcgcg
    15781 gctgtccagc aggttcagct cgtcgcgggt gcgtaatacc agttccacat caccgcgctg
    15841 ttcgagctgc cgcctgatgg cagaaccgac cattccgcga tggccagcga taaaaatgcg
    15901 ttgtctactc atgcttatga ctccagcgcg atcgccacgt cgtagccgtg agatttcagc
    15961 agagagtgtt ttttcgccgc ttcgaggtca ttagccacca tttcagacac catctctctg
    16021 agggtgattt ccggtttcca gcccagtttt tcgtgcgctt tggtcgggtc gccgagcagc
    16081 gtttcaactt cagccggacg gaagtaacgc ggatcaacgg cgataatcac atcacccggt
    16141 ttaacgcccg gcgcgtcatg cccggtgacg gaaaccacaa tgcccttctc ttcaacaccc
    16201 gtgccttcaa agcgcagttt gatgcccagc tgtgccgctg ccatttccac gaactgacgc
    16261 acggagtact gaacgccggt cgcgataacg aaatcttccg gctgttcctg ttgcagcatc
    16321 atccactgca tttttacgta gtctttggcg tggccccagt cacgcaggga atccatattg
    16381 ccgaggtaca ggcacgactc cagcccctgg gcgatgttgg cgattgcgcg ggtgattttg
    16441 cgggtaacga aggtttcgcc gcggcgcggg gattcatggt tgaagagaat tccgttacaa
    16501 gcgtacatgc cgtaggattc gcggtagtta acggtgatcc agtaggcgta cagtttggcg
    16561 accgcatacg gagatcgcgg gtagaacggc gtggtttctt tctgcggaat ttcctgcacc
    16621 agaccgtaca gttcagaggt ggaagcctga tagaaacggg ttttcttttc cagaccgagg
    16681 aagcggatcg cctcgagcag gcgcagcgta cccatcgcat cgacgtctgc ggtatattcc
    16741 ggtgactcaa aagaaaccgc aacgtggctc attgcgccca ggttgtacac ttcatccggc
    16801 tgcacttcac gcaaaatgcg cgtcaggttg gaggtatcac tcaggtcgcc ataatgcaga
    16861 tggaatttcg ggttgcaggt gtgcggatcc tgataaatgt gatccacgcg ctcggtgttg
    16921 aatgacgatg cacgacgctt aataccatgc acctcgtaac ctttttccag cagaaactct
    16981 gccaggtaag aaccgtcttg tccggttaca ccggtgatga gagcgacttt tgacatgtct
    17041 tattcctctg tatttttgaa tttattcagt ttcaacgcgt tcgcgtatca ccactgcggg
    17101 attcccccgg caaaccacat ttgccggaag cgatttaaaa acactgcttc gtgcacccac
    17161 gacggtgccg tcgccgattg tgacgcctgg ggcaacaaag acatcggttg ccagccagca
    17221 tttctcgcca atcacaatag gcgtggcgtt aatggtgaaa tgttgacttg catggtcgtg
    17281 gctcccggtg cataaataac ttttttgcga tatcaccgaa tgtgcgccaa tggttatttc
    17341 accgagggta tataaattga cctcatcgcc gacccacgcg taatcaccta aggttaattt
    17401 ccacggatag gtaattttta ctgacggacg aataactacg ttttttccta tttttgctcc
    17461 gaataaacgt aataaaaaag cccgccagcg atacaatact tgtggcgacc aggcaaatat
    17521 tgttgcctgt actgcccacc ataattgcac tttaatagcg ttgccgcccc ggaacccttt
    17581 cggcaccgag aatccactta aatcctgcat cgttttccct tatattcaga ctttgttata
    17641 taaggctttc gtctttgagg tagtacgttg gcgtaaatgc caggataatt cagcccaaaa
    17701 gccaggtaca tgtaatattt gtcgttggac ttttttcgcg tcagcacaca attccatatt
    17761 attggtggta gataccccac ccatggaaaa ttcagacacc aggccattga gttttttaaa
    17821 tgcataaccg gctttataca ttttggctgc cagcgcgtag tcggaagaaa ctttatattc
    17881 caggtcataa cgccatttct tcaagccgga taccgggaaa aatatcgcct gatgactggc
    17941 gggcaggctg tgataaatat accagcccgg tttggcgcta cgtttaattt tatgcccgtc
    18001 gccaaaatcc agcagcgcat cgccggtgat catcacgtta tctttttgca tttttaactt
    18061 acggacaaaa tttgcggcat tctgatgaaa aatatcgccc gaattgagaa acaacgcgaa
    18121 cttgccttgt gccatcgtaa tgcctttgtt catggcgtca tagataccgt tatctggctc
    18181 gctgacaaag cgtaggttat agataccatt gagattttcc agatactcac gggtgccgtc
    18241 attggaaccg ccatcgacaa caatccattc gaagctgata tcttccgcct gcgccagatg
    18301 cgccagcgag gcatgtgttt tgactatccc ttcgaggtta cgaaacgcga cagtgattat
    18361 gctaagcaac atgttcttat tcttacctcg taatatttaa cgcttttcgc aaaataaacg
    18421 gacagacgat taaaaatgca tattccgggc taaatatcga accggtaaaa aacagcgata
    18481 ccggagtaaa aagatataat tgcacgcgaa aattgcggtt atcaccaaaa gcgttgatca
    18541 tcatttttat cactttcccc atgtaccaca gcgataaaaa caccgcgaac caggaaaaat
    18601 aaataatcag cagatacaaa ccattgtcta tggtttttcc gacatccgca ccgttaaata
    18661 ttccgaatga tgcgacatat tcataaagtg agccaaatct gactacaccg tcaatatggg
    18721 tcaaagaata accgaccatc accagcggac cgacaatacg ataatacgat gacgatcctt
    18781 ccgtacctaa atcgcccaga cgggtggaaa tataaggaaa cgcgattacc acaccaacca
    18841 ggaatacagc cagagaaatc aatgctaacg gtaacttttt cttaatcgcc tctttattca
    18901 gatattggaa tacccactcc agcaaataaa acaggataaa ggtcataacc cctgaaaacg
    18961 atccggataa tattatccct gcgagaatca tagcatcggt tttaggcgtt ttgataccaa
    19021 actgtttgat gctgagccaa attgagatta atgccagagc gaaaaatgcc ggttcgaaat
    19081 aaagtgctgt cgtgcgcttg ccgccgaatt tgatgaaatt cagcacatag ctgttgctgt
    19141 aaatcagata tttcgaaatc gactccataa tactgctgcc gccggtgagg ataatttgcg
    19201 ccatctccac cgccgccagc gccaccacca gcccgaccac cagataaaag aaacgtaata
    19261 tcttgcgatg gttgtgtggc gaaattgttt taaaacgaat actccacacc atgccaataa
    19321 tgatcacaat atagacaaac agcatggttg aagtgacgta tttaccggca tccagcgact
    19381 gaccaaacag gtagttaaac gccgtgagtc ccgcaccaat ccctaaggca atcatcaatt
    19441 ttttaacgct gatgcgttct aaaaacagca gtaacaagac aggtaaaaaa gtgacgatag
    19501 tgatggggaa actttcgcca agctgggcga ttttgacgtt aaccagcagg tagatcagcg
    19561 gcagcagcag gtagctacag attctgatag aagttgacat actcctccag catctgttgt
    19621 ccactgtagg cggcgcggct gcgttggctg aactcagcca gcgtggtacc aaatatcgcc
    19681 tgcgcgattt ccggtttgct taactgcacc agttgcagca cctcttcttc actgacggtt
    19741 ttaccgccgg atttttgcag cacttcacgc gccgcatcgc tatgggtggc aatcaccggc
    19801 acgccaatcg atagcgcctc acacaaaatc agcgggtagt tatcgacgcg agaactgaac
    19861 accagcgcat ccatctgatt gagcgcgctc atcagcttac gtttgtcggt ttcaaagccg
    19921 tgattaacca cgttaccagc ggtgaacggc gagaacttac caaaggtatg cagttcgatt
    19981 ttgtcgccca gcgccatcat ctcacgcacc agttgctggt tagttttgcc gtcgtaacgc
    20041 aggtcatgcg ccaccaccgc gattttcggc ttaccctggg tttcgcgcac cggaggcaga
    20101 tccgccagaa tcgcttcggt tgccatatca ataccgttat tgataatccg gcaacgccct
    20161 ggaccgtaca ggctattgaa agcgtcagcc acatgctggc tgggggaaat aaactgacag
    20221 cccagcgcca gcatctcacg gaataactgg cgtttgcccg ccaccagttg gtgtgcgcga
    20281 tcaatcttca ccggcggata attatttaag gtcgggcatt tctggcagcc cgttttccag
    20341 ccttcacaac cgtcggtaaa ggcgcagcgt ccggtaacgc tccagtggtc gtgcagcgtc
    20401 cagaccagag tgacgtccgg tttatggttt ttcacctttt cgcaaaagcg caccacgctc
    20461 ttaagattta gccagtagct gtgcagcaca tgaaaatgca ggaccaccgg acccggtgta
    20521 cgagtaatgg tgcgatataa ctcattgaaa ttgccaaaca gatcgcgatt aaacagacga
    20581 aacaggacaa tattcgccat tgcagtcatc cgcggcgtat gtttgatgac ctgcggatag
    20641 ttttgatggc tgacgctctc tttgccgcct ttgccgtaac cgtagacaaa atgtgacgct
    20701 agcccctgtt gcagcgcacg ctggtggaga tctaacgcca cacctgctgc cccgccttcc
    20761 gccagtcgca cattaaattg caaaatattc atttaattac cttcactcgc gctttttctc
    20821 ccaccaccag cgcgttgtcc gggacagagt cgagcaccac gctgcccgca cccacggtga
    20881 cgttgttacc aagcgtgata tcaccaagaa taataacgtt ggcaccgagt tcgacgccgt
    20941 tgccaatatg tggacatgcc atgttatcgg caccacgatt gccgatagtg acgccgtggc
    21001 gaatggtgaa atcatccccc gccaccacgt ttttattgat cactacggcg taaccgtgat
    21061 ggatagtaaa gcggcggcca atggtcgcgg cagcctggat ttcataaccg aaaaagcatt
    21121 cggtgataat gcgatacagc accagcagag gggccgccca aatattgttg aggacgttct
    21181 ttttgcgcca caccgaacaa aaatgagcga cgcgataggc tagaaccatg cagcacggac
    21241 gtaaactcca gctgttggcg cgcagatctt ccagcatctt tagcgccccc gaataccatc
    21301 agccagacgt ttgccgttac gcaccgacag cagcgtcagc aaagtgcgcc aggtcatacg
    21361 tttattgcgg atctggtaga gggtaaacag ctgatatttt ttgctggcgc ggtcgaattt
    21421 gtctttgtgc ttgcggtaaa agtggaagta tccggagaat tttttcggtg acgaggtgat
    21481 ctgcatttca ccgtgattga tatgcaggat ctgcgtcgcc tcttccactt tccacggttc
    21541 gccatactcc accaccatcc gcaggaagat gtcgtaatcc tgtgccgctt tcagttcggt
    21601 atcgaacaag cactctttga aacgccacgc ccaggtaaaa acctgattgc caatgatatt
    21661 gcgtttatag aacaggcggc gtgagtacgg cgatttggga tacagtggta agcttgctgg
    21721 ttgggaataa acttctccct ggcatacgta gtcgttagcg tacaaaaagg cgtgagtgac
    21781 cagttgctgt ttatgggcga ggaagacgct caggcggttg ggcgtccatt catcatcgtc
    21841 atcgatcccg gtaatgtatt ccccttgcgc cagcataatc gcctggttac gtaccgcgca
    21901 cgccccgctg ttaatgtcgt tgtgaatgta ggtgatacgc ggatcgttga gggcagtgac
    21961 gtactgttgc agttgttccc aggaggtgga gcaatcatcc acgatgatca tctcccagtt
    22021 gctgtagtcc tggcgcagga ccgattttat cgcccgaatc gccagttgtt ggcggttcca
    22081 ggtcggcata tagattgaaa tcagcggatt gtctttcata ggttgttctc ccggagtatc
    22141 gagatgttct gccggtttat aagcttgttt acgggttctg ttgccggatg cggcgtgagc
    22201 gccttatccg gcctacggag ctggtttgct tgccggatgc ggcgtgaagg taggtcggat
    22261 aagatgcgtc agcatcgcat ccgacagctg atgtcgttat ttcgcatccg acttatattc
    22321 gtattcgtaa tagccataat cctgatacgc gctggcgcgg cggaagatgg agttcagaat
    22381 cacccctttc accggaatac cgttttgctc aaagcggctc agactggttt ccacttcttt
    22441 caatgtgttg accgcataac gcgccaccat taacgtggtt ccgacatgac gaccaacaat
    22501 tgccgcatcg gtcactgcca gaatcggcgg cgtatcaatc aacaccaggt catagttttt
    22561 actcgcccag ttcaccagtt cggcaaagcg ttcgctcatc aacagttcag aaggatttgg
    22621 cggtacctga ccgcgcggga tcaggtcaaa tttggcaata gaggtcggtt tagctgcggt
    22681 agtaatatcg ccctggccaa tcagaatttc cgacaggcca ttgacgttat tggtacccaa
    22741 cagctcgtgg gtgtagcctt tgcgcatatc gcagtcgatc aacaacaccc gtttattggt
    22801 ctgactgatc accgccgcca ggttggcgca gacaaaggtt ttaccgattg acgggctcac
    22861 ccctgtcatc atcaacacat tgttctgcgc ctgcatcatc gcgaagtgca gactggtacg
    22921 cagactgcgg atggcttcaa tcgccagatc ggttggattc cccaccgcca gtagctggct
    22981 ctgtttatag cgtttaatcc ctttgatggt tttgacgcta tcgcgcgctt tctgccattc
    23041 cgacaacggg atgctggcat agacgctgat accatgttct tccagcacct gcgggctttc
    23101 gatgccgcga ttaaacaacg agcgcagcag cacacccacg atagagagca tcaggccgag
    23161 gataattgcg ccgaggataa tcagtccttt cttcggtttc agcacgccag gctgggtgat
    23221 tgccgggtca acaatgcgca catcgccgac ggtgctggct tcggtgattt tcagctcctg
    23281 ctctttattc agcagttgca tatagacctg ctgaccagac tcgacatcgc gggtcagacg
    23341 gacaatttcc tgctgggttt tcggcatcgc cgttacgcga ccattaagtt tggctttttc
    23401 gtcttccagc gcctgacgtt tctccagcag cgtgcggtac gcggggtgaa ctttggtgta
    23461 cagcttggag atttccgcct ctttaaaggt cagttcgttc aactgcgcgt cgatgttcac
    23521 catcgaatcg agcaccgctt tcgcttccag cggcagatca acagaatctt tatcctgacg
    23581 gaaggcattc agtttgtttt cggcaacatc gaggcggcta cgtacttccg gtaactgttg
    23641 cgcgaggaaa gcgaggcttt tcgacgcttc cgccgatttg cgctcaatat tttgttcctg
    23701 atagttacgg gcgatgctgt taagaatgtc gcggatctgt tcgcgatctt caccggtata
    23761 agtcaggctc agtacgcctg cgtctttgcc gttctccgtt accgtcaggc tgttttgcag
    23821 ttggttgatc atccccaacg tggagtattt ggtgacggta aactcactgc ccgggcgggc
    23881 gtgaatggct tcaaccatca gcgtgacgcc ttcttttttc agaatctggc ccgcttgccc
    23941 acgggcgcta aagccgccat cactgctcag agtgtagttt ttgtcatcca gcacattaag
    24001 cgtaaacacc tggtccgcca tctctttcgg gcggttaaag gtggtcactt tcaccgtctc
    24061 gttctgacgt cccatcaggc gatcccaacc cgcaccgaaa atcgggaacg tgtttttgct
    24121 caccgcaata tcgaggtcta gatcgtccac cgttttacca agcaccagac gcgaacgaat
    24181 caactggatc tcggcgtccg atgcaggcgg tttgttggct aatgccgagc caatgtcctg
    24241 cactaacgaa ttgccgctgt tttgttcgat ttgtaccagt gcgtcggcac tataaatcgg
    24301 cgtggcgaag aaagtgtaaa ccacagcaca gagggcaaat acagcggtga tgccaatcac
    24361 ccaccagcgc gcttcaatga cggtgccgac caggcgacca atatcgattt catcactgcc
    24421 cgttaccgga gcggcatgtt gttttacttt ttctgtcatt cttatacctg ctctgcgttc
    24481 aatgcctgct cccactggcg ggcagaccgt tcaagtaatg tgtacaccgc tgcaaacgtt
    24541 tcccggcttt tgcgatacgg atcggggatt tcacattcgt tatcccagtg accaaacagc
    24601 atcactttgc cgcgcatctc aggtgccatc tcgcataagc gttcgatatg gcgcttttcc
    24661 atggtcaaaa tcaggtcata gttgcgacac aggcggcggc tgatttgccg tgcacagtga
    24721 ccttccagag acagttgatg ttctgcggcg acgctgatag cggtaggatc agcgccctta
    24781 ccgaccagcg cgccgagtcc agcggactcc actttcagct ccgggtgata acgttgcagt
    24841 aagcgttccg ccgtcgggga acggcaaata ttgccgacac agacaactaa gatgttgtta
    24901 aacatgacga ttaccagtta tgaatgtcgc tggctgtatc cgtcatgtaa cggacaccgc
    24961 taatagttgg cagcaactga ttgatcagac ggttccagcg ggaaaccggg gcggtggtga
    25021 catacaccac gtcataaggt tgcaggcgga attctgtcgc catcaccagc gacgtcgcat
    25081 cggacatatc cagctggtag atattggcaa tcttgccgtt acgcccgccc tcgcctttca
    25141 gcggacgaat gacaaagatg ccgctggcgt tggaggtggt catgtcgata ccttcggcat
    25201 tgcccagggc ttcagtcagg gtcatgccgc taaagtccat tttcagggtg ctctgtttct
    25261 tcacttcacc catcacaaac actttcagat catcattacg cggcacgtag agaatatcgc
    25321 cggggtaaag cagtcggttc tgattgaggt cgccgttttg catcagcgcc tgcaaagaaa
    25381 tgcgttcttc acgaccattg tgtgttagca ccacgttgcg ccagtcagcg gtgtcggtca
    25441 ggccacctgc ggcgttgatg gcgtcgagaa tggtcagtgg tacgttggtg atcgcctgtt
    25501 gaccggattt attcacctga cctgagatat aggccttttg cgagcggaag gcggcgatat
    25561 taacatccac ctgcgggtca gcgatgtacg tcgctaagcg cccggtaata tcactgcgga
    25621 tttcagcgag cgttttcccg actacgtgga ccttgccgat atacgggtaa aacatagtgc
    25681 cgtcaggctg tacccagttg ccggtgtcgc tggagctgcg gtactgaccg gctggcgtgg
    25741 tgagttccgg gtgatcccag acggtgacat taagaacgtc ccccggcccg acgcgatact
    25801 ggtaattcgc gatctcactt tccagcgtca tattggggcg cgctacattc gggcgtggac
    25861 gtaattgatc aatcaggcgc ggggtcaacg gataaacatt caccattttg tcgagatcga
    25921 aatcagcgtc ctgctgtttg atgacgtctt tgcccatcgt cgacatattg ctgcccggaa
    25981 gtactgtgca accgcttatc aaggttactg acaccaataa tggcatcaat ttcattttgg
    26041 atttcatcat tgtttattta tcactttggc agagtaatta tcctgtgcac tattaatagc
    26101 aatgtcgcca tgcacattta ccttgcagtt aattgaataa aaatttaact ggcatcagtc
    26161 ctaaaaaaat tgatttcatc cgcaggctat tgacagaata attcagactg gtctttcagg
    26221 catccagaca cgctaccgcc cctggctttt tagctaccaa tacactgatt tagcttaatt
    26281 tttcaaaccc tctctgcata caatcgttga tgagaaaggg ttattgcgga aattaacttc
    26341 cgaatataag gtgacattat ggtaattgaa tattggctct ccaataatgc aggaggaagt
    26401 gttacagcta acggaatagc aggcaaggta acaattcggc aattggctat
//
LOCUS       DAZGVQ010000063        26445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000063 DAZGVQ010000000
VERSION     DAZGVQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.063"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..670)
                     /locus_tag="ACHLAG_002497"
     CDS             complement(<1..670)
                     /locus_tag="ACHLAG_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP0655705.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFH"
     gene            complement(830..1042)
                     /gene="rpmE"
                     /locus_tag="ACHLAG_002498"
     CDS             complement(830..1042)
                     /gene="rpmE"
                     /locus_tag="ACHLAG_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFP0655706.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            1245..3443
                     /gene="priA"
                     /locus_tag="ACHLAG_002499"
     CDS             1245..3443
                     /gene="priA"
                     /locus_tag="ACHLAG_002499"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFP0655707.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3599..4624
                     /gene="cytR"
                     /locus_tag="ACHLAG_002500"
     CDS             3599..4624
                     /gene="cytR"
                     /locus_tag="ACHLAG_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFP0655708.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKVAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4716..5675
                     /gene="ftsN"
                     /locus_tag="ACHLAG_002501"
     CDS             4716..5675
                     /gene="ftsN"
                     /locus_tag="ACHLAG_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFP0655709.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5768..6298
                     /gene="hslV"
                     /locus_tag="ACHLAG_002502"
     CDS             5768..6298
                     /gene="hslV"
                     /locus_tag="ACHLAG_002502"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFP0655710.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            6308..7639
                     /gene="hslU"
                     /locus_tag="ACHLAG_002503"
     CDS             6308..7639
                     /gene="hslU"
                     /locus_tag="ACHLAG_002503"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFP0655711.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            7706..8632
                     /gene="menA"
                     /locus_tag="ACHLAG_002504"
     CDS             7706..8632
                     /gene="menA"
                     /locus_tag="ACHLAG_002504"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP0655712.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8725..9210
                     /gene="rraA"
                     /locus_tag="ACHLAG_002505"
     CDS             8725..9210
                     /gene="rraA"
                     /locus_tag="ACHLAG_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFP0655713.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(9295..9540)
                     /gene="zapB"
                     /locus_tag="ACHLAG_002506"
     CDS             complement(9295..9540)
                     /gene="zapB"
                     /locus_tag="ACHLAG_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFP0655714.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9965..10810
                     /gene="glpF"
                     /locus_tag="ACHLAG_002507"
     CDS             9965..10810
                     /gene="glpF"
                     /locus_tag="ACHLAG_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFP0655715.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10833..12341
                     /gene="glpK"
                     /locus_tag="ACHLAG_002508"
     CDS             10833..12341
                     /gene="glpK"
                     /locus_tag="ACHLAG_002508"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFP0655716.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            12476..13486
                     /gene="glpX"
                     /locus_tag="ACHLAG_002509"
     CDS             12476..13486
                     /gene="glpX"
                     /locus_tag="ACHLAG_002509"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP0655717.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13583..14329
                     /gene="fpr"
                     /locus_tag="ACHLAG_002510"
     CDS             13583..14329
                     /gene="fpr"
                     /locus_tag="ACHLAG_002510"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFP0655718.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(14334..14762)
                     /gene="uspD"
                     /locus_tag="ACHLAG_002511"
     CDS             complement(14334..14762)
                     /gene="uspD"
                     /locus_tag="ACHLAG_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFP0655719.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14789..15088)
                     /gene="yiiS"
                     /locus_tag="ACHLAG_002512"
     CDS             complement(14789..15088)
                     /gene="yiiS"
                     /locus_tag="ACHLAG_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFP0655720.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(15300..15740)
                     /gene="yiiR"
                     /locus_tag="ACHLAG_002513"
     CDS             complement(15300..15740)
                     /gene="yiiR"
                     /locus_tag="ACHLAG_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP0655721.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15841..16440
                     /gene="yiiQ"
                     /locus_tag="ACHLAG_002514"
     CDS             15841..16440
                     /gene="yiiQ"
                     /locus_tag="ACHLAG_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFP0655722.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16548..17315
                     /gene="tpiA"
                     /locus_tag="ACHLAG_002515"
     CDS             16548..17315
                     /gene="tpiA"
                     /locus_tag="ACHLAG_002515"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFP0655723.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(17370..18125)
                     /gene="cdh"
                     /locus_tag="ACHLAG_002516"
     CDS             complement(17370..18125)
                     /gene="cdh"
                     /locus_tag="ACHLAG_002516"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFP0655724.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(18232..19221)
                     /gene="sbp"
                     /locus_tag="ACHLAG_002517"
     CDS             complement(18232..19221)
                     /gene="sbp"
                     /locus_tag="ACHLAG_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFP0655725.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19541..20503)
                     /gene="pfkA"
                     /locus_tag="ACHLAG_002518"
     CDS             complement(19541..20503)
                     /gene="pfkA"
                     /locus_tag="ACHLAG_002518"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP0655726.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20684..21586)
                     /gene="fieF"
                     /locus_tag="ACHLAG_002519"
     CDS             complement(20684..21586)
                     /gene="fieF"
                     /locus_tag="ACHLAG_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFP0655727.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTLMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21735..22235)
                     /gene="cpxP"
                     /locus_tag="ACHLAG_002520"
     CDS             complement(21735..22235)
                     /gene="cpxP"
                     /locus_tag="ACHLAG_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFP0655728.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            22385..23083
                     /gene="cpxR"
                     /locus_tag="ACHLAG_002521"
     CDS             22385..23083
                     /gene="cpxR"
                     /locus_tag="ACHLAG_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFP0655729.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            23080..24453
                     /gene="cpxA"
                     /locus_tag="ACHLAG_002522"
     CDS             23080..24453
                     /gene="cpxA"
                     /locus_tag="ACHLAG_002522"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFP0655730.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24559..25233)
                     /gene="yiiM"
                     /locus_tag="ACHLAG_002523"
     CDS             complement(24559..25233)
                     /gene="yiiM"
                     /locus_tag="ACHLAG_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFP0655731.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(25382..26365)
                     /gene="kdgT"
                     /locus_tag="ACHLAG_002524"
     CDS             complement(25382..26365)
                     /gene="kdgT"
                     /locus_tag="ACHLAG_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFP0655732.1"
                     /translation="MQIKRSIEKISGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
BASE COUNT         6471 a         6953 c         6848 g         6173 t
ORIGIN      
        1 ggtggaacgt ctgtgtgcgc ccgaagcggt ccaccagccc ggtcagtacc cggtacggcg
       61 gcagcggcgc aggcagcacc tcatccgctt ccggcacccg ctcacaccag ccgagcagcc
      121 accacggccc ctgcggactg tttgtcgcca gataacgatg cggacttaag cggagttctt
      181 ccggcagcgc ctgccagagt gcggccagcc ggtgaccttc atccagtttc gccacgccgc
      241 cgcgcaccag ccagagtgat tcgctgcggc tgtaaccgtc ctcaccggga aacaggtgct
      301 caaaatacag gcttctgccg ccgttatcac tgagtatcag tgtgttatcg cgcagctgta
      361 agcggatatc cgcaggcatt ttccagccgg ggccgaggct ccccaccggc gcgggcgttt
      421 ttgtccggta actgctgtag gtgcgggaga gaatgaacgg cagcgggccg ggcagggcga
      481 tgtcggtttc accgggaagg acctttgcac ccagcagcgg attgaccgga tggccggacg
      541 tcaccccgcc ggggcacacc gaacaggcca cgccggtggg ggcaccaatg cgcaccccgg
      601 ctgaaccctg gacaatgcta ccgccatact gcgtcatgtc gccctgacgc gccgccggtt
      661 ttccgctcat cgtaaaatcc ttttgaaata aataagaacc agaagcctag cgccagtcaa
      721 caacaggaaa tgataatgag ttaacacccc ggaatttttc agaatttata aaaggcaaat
      781 tacaggcaca aaaaaagcgc cgtgcggcgc ttttttcgga aatccggtct tatttgctgc
      841 ccgggatgtt gaaacgcttg ttgaagcggt caacacggcc accggtagca acatcacgct
      901 gtttgccagt gaagaacggg tggcacttgc tgcacacgtc gaggttcagg tcatgaccaa
      961 cggtggagcg gattttcatt acgttaccgc aagagcagct agcagtaatt tcttcgtatt
     1021 tcgggtgaat atcttttttc atgggaaaac ctcggtttaa ggccgcgtcg ctcttccagc
     1081 cctaacgcca gacaccacgc gatgttaaaa gtatagcttc aatacgatca tttcgtacga
     1141 aggcgcgaaa tcatacagaa attaaccagc gtatgcaaac tgatccgcac tcttctacgg
     1201 caatgtgtat actaacccac cgaatttcaa gtcaggatga tgctatgccc gttgcccacg
     1261 ttgccttgcc cgttccgctt cctcgtacct ttgactatct gctgccagaa ggcatgacgg
     1321 ttaaagccgg gtgtcgcgtg cgcgtgccgt ttggcaaaca gcaggagcgc atcggggttg
     1381 tggtgtcagt tagcgatgtc agtgagctgc cgcttaacga gctgaaagcg gtagtggaag
     1441 tgctggacgt tgagccggta tttactcact ccgtctggcg gttactgctc tgggcggcag
     1501 attactatca tcatccgatt ggcgatgtgc tgtttcatgc cttgccgatt ttactgcgcc
     1561 aggggcggcc tgcggcgaac gcgccgatgt ggtactggtt tgccactgaa caaggccagg
     1621 cggtggatct gaacagcctg aaacgctccc ccaagcaaca acaggcgctg gcggcgttac
     1681 ggcaaggcaa aatctggcgc gaccaggtcg ccacgctcga atttaatgat gccgcgttgc
     1741 aggcgctacg caaaaaaggt ctgtgtgatt tagcaagtga aacaccagag tttagcgact
     1801 ggcgaacgaa ctatgccgtt tctggtgagc ggttgcgatt gaataccgaa caagccaccg
     1861 ccgttggcgc aattcatagc gcggcagata ctttttctgc ctggctgctg gcgggcgtta
     1921 ccggttccgg taaaacggag gtttatctca gtgtactgga aaacgtgctc gctcagggca
     1981 aacaggcgct ggtgatggtg ccggaaatcg gcctgacacc gcaaactatc gcccgttttc
     2041 gtgaacgttt taatgccccc gtggaagttc tgcattccgg cctgaacgac agcgagcggc
     2101 tttcggcgtg gttgaaagcg aaaaatggcg aggcggcgat tgtgatcggc acccgctccg
     2161 cgctgtttac gccgtttaaa aatctcggcg tgattgtcat tgatgaagag cacgacagct
     2221 cctacaagca gcaggaaggc tggcgctatc atgcccgcga cctggcggtg tatcgtgcgc
     2281 atagcgagca aatcccgatt attcttggct ccgcaacgcc cgcgctggaa acgttatgca
     2341 acgtccagca gaaaaaatac cgcctgctgc gcctgacccg tcgggcaggg aatgcgcgtc
     2401 cggcaattca acatgtgctg gatttaaaag gtcagaaggt gcaggcaggt ctggctccgg
     2461 cgttaatcac ccgtatgcgc cagcatttac aggccaacaa tcaggttatc ctctttctta
     2521 atcgtcgggg ttttgcgcct gcgctgctgt gccacgactg cggctggata gccgaatgcc
     2581 cacgttgcga tcactactac acgctgcatc aggcgcaaca gcatttgcgc tgtcaccact
     2641 gtgacagtca gcgtccggtg ccgcgccagt gcccttcctg cggttccacg cacctggtcc
     2701 ccgtggggct gggcaccgaa cagcttgaac agacgcttgc gccgatgttc cccggcgtgc
     2761 ccatttcacg tatcgaccgc gataccacca gccgcaaagg ggcgctggaa cagcaactgg
     2821 cggaagtgca tcgcggcggc gcgaggatct tgattggcac gcaaatgctg gcgaaaggtc
     2881 accactttcc ggatgtgacg ctggtggcgt tactggatgt ggacggcgcg ctgttttctg
     2941 ccgattttcg ctcggcagaa cgtttcgccc agctttacac ccaggtcgcc ggtcgtgccg
     3001 ggcgtgcggg taaacagggc gaagtggtgc tgcaaacgca ccatccggaa catcctctgt
     3061 tgcaaacgtt gctctataaa ggctacgacg cctttgccga acaggcgctg gctgagcgac
     3121 gcatgatgca gctgccgccg tggaccagcc atgtgattgt gcgtgcggaa gatcataaca
     3181 atcagcatgc gccgctgttc ctgcaacaac tgcgtaatct gatcctttcc agcccgctgg
     3241 cagacgacaa actgtgggtt ctcggcccgg ttccggctct ggcacctaag cgtggtggcc
     3301 gctggcgctg gcagatcttg ttgcagcacc cttcccgcgt gcgcttgcaa cacatcatta
     3361 gtggtacgct ggcgcttatt aatacaatac cggattcccg caaggtgaaa tgggtgttgg
     3421 atgtcgatcc catcgaaggc taatgcgcaa acgttgcgag gcggatcgaa aaattcaata
     3481 ttcatcacac ttttcatgaa aattctgtaa ccgttttcac gcgctatctg ctaaaaatgt
     3541 tgccgatgtg aagtaaacat ggatgtagta cgcctgacgt gccaggcgag gagtgagtgt
     3601 gaaagcgaag aagcaggaaa ctgccgcgac catgaaagac gttgccctca aggcaaaagt
     3661 ctctacagcg actgtctccc gagcattaat gaatcccgat aaagtctccc aggccacccg
     3721 taatcgggtt gaaaaagtgg cccgggaagt gggttattta ccgcaaccta tggggcgcaa
     3781 cgtcaagcgt aatgaatccc gcaccattct ggtgattgtc ccggatatct gcgatccctt
     3841 ctttagcgaa attattcgcg gtatcgaagt tacggcggca aatcacggat atctggtgct
     3901 gattggcgac tgtgcgcatc aaaatcagca ggaaaaaacc tttatcgatt tgatcatcac
     3961 caagcaaatt gatggcatgt tgctgctggg ctcaaggctg ccgtttgatg ccagcattga
     4021 ggaacagcgt aatctgccgc cgatggtgat ggcgaacgaa tttgcaccgg aactggagct
     4081 gcctaccgtt catatcgaca atctgaccgc cgcatttgat gcagtaaatt atttatatga
     4141 gcaagggcat aaacggattg gctgtatagc cggtcccgaa gagatgccac tgtgtcacta
     4201 ccgcctgcaa ggctatgttc aggcgctgcg tcgctgcggc attatggttg atccgcaata
     4261 catcgcccgt ggcgacttca ccttcgaagc cggaagcaaa gcgatgcagc agctgcttga
     4321 tcttccacaa ccgcctactg ctgtcttctg ccatagcgat gtgatggcgc tcggcgcact
     4381 ttctcaggca aaacgccagg ggctgaaagt cccggaagac ctttccataa tcggttttga
     4441 taacatcgac ctgacgcaat tttgtgatcc gccgctgaca accatcgcgc agccgcgtta
     4501 cgaaatcggt cgggaagcta tgctgttatt gcttgatcaa atgcaggggc aacacgttgg
     4561 cagtggctct cgtttaatgg actgcgaact tatcatccgg ggatcaacac gcgcgttacc
     4621 ttaaagtaaa cctttaagac tgccgtgact ggtcaaaggc ccgccgctta agtaacatgg
     4681 cgggctgacg aacgaataaa tacagcgaaa cgatagtggc acaacgagat tatgtacgcc
     4741 gcagccaacc ggcaccttcg cggcgaaaaa agagcacctc acggaaaaag caacgaaatc
     4801 tgcctgcggt ttctcccgct atggtcgcta ttgctgccgc cgttcttgtg acctttatcg
     4861 gtggtctgta cttcattacg catcacaaga aagaagagtc cgagacgctg caaagccaga
     4921 aagtgaccgg taacggacta ccaccaaaac cagaagaacg ctggcgctac attaaagagc
     4981 tggaaagtcg ccagccgggt gtgcgtgcgc ccacagaacc ttctgccggt ggtgaagtga
     5041 aaacgccgga gcaactgaca ccagaacaac gtcagcttct tgaacaaatg caggctgata
     5101 tgcgccagca gccaacgcag ctggttgaag ttccgtggaa tgaacagacg ccggaacagc
     5161 gtcagcaaac gctacaacgc cagcgtcagg cgcagcaatt agcggagcag caacgcctgg
     5221 ctcagcagtc gcgtacgact gaacaaagct ggcagcagca gacgcgtacg tcgcaagccg
     5281 cgccggtaca ggcgcaaccg cgccagtcta aaccggcttc tactcagcag ccgtaccagg
     5341 atctgctgca aacccctgcg cacacgaccg cgcagtcgaa gccgcagcaa gctgcgccgg
     5401 ttgctcgtgc cgctgacgcg ccaaaaccga cggcggagaa aaaagacgaa cgccgctgga
     5461 tggtgcagtg cggttcgttc agaggcgcgg aacaggcaga gacggtacgt gctcagctgg
     5521 cgttcgaagg atttgactcg aaaatcacca ccaacaatgg ctggaatcgt gtggtcattg
     5581 gcccggtgaa aggcaaagag aacgcagaca gcaccctcaa tcggttgaag atggcgggtc
     5641 atacaaactg cattcggctc gccgccgggg gttgaaaccc tcaaaatccc ccccatctat
     5701 aattgcatta tgccccgtac ttttgtacgg ggtttgtact ctgtattcgt aaccaagggg
     5761 tcagctcgtg acaactatag taagcgtacg ccgtaacggc catgtggtca tcgctggtga
     5821 tggtcaggcc acgttgggca ataccgtaat gaaaggcaac gtgaaaaagg tccgccgtct
     5881 gtacaacgac aaagtcatcg cgggctttgc gggcggtact gcggatgctt ttacgctgtt
     5941 cgaactgttt gaacgtaaac tggaaatgca tcagggccat ctggtcaaag ccgccgttga
     6001 gctggcgaaa gactggcgta ccgatcgcat gctgcgcaaa cttgaagcac tgctggcagt
     6061 cgcggatgaa actgcatcgc ttatcatcac cggtaacggt gacgtggtgc agccagaaaa
     6121 cgatcttatt gctatcggct ccggcggccc ttacgcccag gctgcggcgc gtgcactgtt
     6181 agaaaatact gaactgagtg ctcgtgaaat tgctgaaaag gcgttggata ttgcaggcga
     6241 catttgcatc tataccaacc atttccacac catcgaagaa ttaagctaca aagcgtaagg
     6301 atctcccatg tctgaaatga ccccacgcga aatcgtcagc gaactggata agcacatcat
     6361 cggccaggac aacgccaagc gttctgtggc gattgctctg cgtaaccgct ggcgtcgcat
     6421 gcagctcaac gaagagctgc gccatgaagt gaccccgaaa aatatcctga tgatcggccc
     6481 gaccggtgtc ggtaaaactg aaatcgcccg tcgtctggct aagctggcga atgcgccgtt
     6541 catcaaagtt gaagcgacca aattcaccga agtgggctac gtcggtaagg aagtggattc
     6601 tattattcgc gatctgaccg atgccgccgt gaaaatggta cgcgtccagg ctatcgagaa
     6661 aaaccgttat cgcgctgaag aactggcaga agaacgtatt ctcgacgtgc tgatcccacc
     6721 tgctaaaaac aactggggac agaccgaaca gcagcaggaa ccgtccgctg ctcgtcaggc
     6781 attccgcaaa aaactgcgtg aaggccagct tgatgacaaa gaaatcgaga tcgatcttgc
     6841 cgcagcaccg atgggcgttg aaattatggc tcctccaggc atggaagaga tgaccagcca
     6901 gctgcagtcc atgttccaga acctgggcgg ccagaagcaa aaagcgcgta agctgaaaat
     6961 caaagacgcc atgaagctgc tgattgaaga agaagcggcg aaactggtga acccggaaga
     7021 gctgaagcaa gacgctatcg acgctgttga gcagcacggg atcgtgttta tcgacgaaat
     7081 cgacaaaatc tgtaagcgcg gcgagtcttc cggtccggat gtttctcgtg aaggcgttca
     7141 gcgtgacctg ctgccgctgg tagaaggttg caccgtttcc accaaacacg ggatggtcaa
     7201 aactgaccac attctgttta tcgcttctgg cgcgttccag attgcgaaac cgtctgacct
     7261 gatcccggaa ctgcaaggtc gtctgccaat ccgcgttgaa ctgcaggcgc tgaccaccag
     7321 cgacttcgag cgtattctga ccgagccgaa tgcctctatc accgtgcagt acaaagcact
     7381 gatggcgact gaaggcgtaa atatcgagtt taccgactcc ggtattaagc gcatcgcgga
     7441 agcggcatgg caggtgaacg aatctaccga aaacatcggt gctcgtcgtt tacacactgt
     7501 tctggagcgt ttaatggaag agatttccta cgacgccagc gatttaagcg gtcaaactat
     7561 catcattgac gcagattatg tgagcaaaca tctggatgcg ttggtggcag atgaagatct
     7621 gagccgtttt atcctataat cgcgttcaat cattttcatc attgtttgat ggggctgaaa
     7681 ggccccattt ttattggcgc gtattatgac tgaacaacaa attagccgaa ctcaggcgtg
     7741 gctggaaagt ttacgaccta aaaccctccc cctcgccttt gctgcaatta tcgtcgggac
     7801 agcgctggca tggtggcaag gtcacttcga tccgctggtc gccctgctgg cactgattac
     7861 cgccgggcta ttacagatcc tttctaacct cgccaatgat tacggcgatg cggtaaaagg
     7921 cagcgataaa cctgaccgca ttgggccgct acgcggcatg caaaaagggg tcattaccca
     7981 gcaagagatg aaacgggcgc tcattattac cgtcgtgctc atctgtctct ccgggctggc
     8041 actggttgca gtggcatgcc atacgctggc cgattttgtc ggtttcctga ttcttggcgg
     8101 gttgtcgatc attgccgcta tcacctacac cgtgggcaat cgtccttatg gttatatcgg
     8161 tctgggtgat atttccgtac tggttttctt tggctggttg agtgtcatgg ggagctggta
     8221 tttacaggct catacattga ttccggcact gatccttccg gcgaccgcat gcggcctgct
     8281 ggcaacggca gtactgaata ttaataacct gcgtgatatc aatagcgacc gcgaaaatgg
     8341 caaaaacacg ctggtggtgc gcttaggtga agtgaacgcg cgtcgttatc atgcctgcct
     8401 gctgatgggc tcgctggtgt gtctggcgct gtttaatctc ttttcgctgc atagcctgtg
     8461 gggctggctg ttcctgctgg cggcaccatt actggtgaag caagcccgtt atgtgatgcg
     8521 ggaaatggac ccggtggcga tgcgaccaat gctggaacgt actgtcaagg gagcgttact
     8581 gactaacctg ctgtttgttt tagggatatt cctaagccag tgggcagcat aactgacaaa
     8641 tatcaattaa caattgatga ttttgccaac agcctacata gcgcgatata ctgaaaattc
     8701 tcgcagcaac tgaatgttaa gcctatgaaa tacgatactt ccgagctttg tgacatctat
     8761 caagaagatg ttaacgtcgt ggaaccgctg ttctccaact ttggcggacg ggcgtcgttt
     8821 ggcggacaaa taatcacggt aaaatgtttc gaggacaacg ggttgctgta cgatctgctc
     8881 gaacagaatg gccgtggtcg tgttcttgtc gtcgatggcg gtggttctgt tcgtcgcgca
     8941 ctggtcgatg ctgaactggc gcgtctggca gtacaaaatg aatgggaagg tctggtcatt
     9001 tacggcgcgg tgcgtcaggt agatgacctg gaagagttgg atatcggcat ccaggcgatg
     9061 gcggcaattc cggttggtgc cgctggcgaa ggcattggcg aaagcgatgt ccgcgtcaat
     9121 tttggcggtg tcaccttctt ctccggcgac catctttatg ccgacaatac cgggattatt
     9181 ctttctgaag atccgctgga tattgaatga tagaaaaggc accgcaaggt gccttttttc
     9241 tgcgttacct gttggcctac acagtaaaga aattacgcgg aagatgaagc gtaatcagac
     9301 ctcttccatg cgacccagca gggcctgcag acgttcctgc cagccgttct gctgttcttt
     9361 cagatggttg ttctcacgct ccagctcttc gcgctgatgc tgggcatttt gaacttcctg
     9421 cgacagtgag ttgttttttt ctttcagctc ttcgatttcc atctgcaaca gagtgatggt
     9481 atcaatcgcc tgctgtactt ttgcttccag tttctcaaac acttctaatg acattgtcat
     9541 acctctcctg aattgcaagg cgttgatgga taaaaatcct cgtcccgatt accggtgacg
     9601 ccttaataaa tacgagcgca ctttagttag ctccgattgt atgaagccgc gccatcgctg
     9661 tccagcggca cgccttgcag attacggttt gccacacttt tcatccttct cctggtgaca
     9721 taatctacat caatcgaaaa tgttaataaa tttgttgcgc gaatgatcta acaaacatgc
     9781 atcatgtaca atcagatgga ataaatggcg cgataacgct cattttatga cgaagcacac
     9841 acattttaag ttcgatattt ctcgtttttg ctcgttaacg ataagtttac agcatgccta
     9901 caagcatcgt ggaggtccgt gactttcacg catacaacaa acattaactc ttcaggatcc
     9961 gattatgagt caaacatcaa ccttgaaagg ccagtgcatt gctgaattcc tcggtaccgg
    10021 gttgttgatt ttcttcggtg tgggttgcgt tgcagcacta aaagtcgctg gtgcgtcttt
    10081 tggtcagtgg gaaatcagtg tcatttgggg actgggggtg gcaatggcca tctacctgac
    10141 cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc attgcattgt ggctgtttgc
    10201 ctgtttcgac aagcgcaaag ttattccttt tatcgtttca caagttgccg gcgctttctg
    10261 tgctgcggct ttagtttacg ggctttacta caatttattt ttcgacttcg agcagactca
    10321 tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct ggcactttct ctacttaccc
    10381 taatcctcat atcaattttg tgcaggcttt cgcagttgag atggtgatta ccgctattct
    10441 gatggggctg atcctggcgt taacggacga tggcaacggt gtaccacgcg gccctttggc
    10501 tcccttgctg attggtctac tgattgcggt cattggcgca tctatgggcc cattgacagg
    10561 ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc tttgcctggc tggcgggctg
    10621 gggcaatgtc gcctttaccg gcggcagaga cattccttac ttcctggtgc cgcttttcgg
    10681 ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc aaactgattg gtcgccattt
    10741 gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca actccttcag aacaaaaagc
    10801 ttcgctgtaa tatgactacg ggacaattaa acatgactga aaaaaaatat atcgttgcgc
    10861 tcgaccaggg caccaccagc tcccgcgcgg tcgtaatgga tcacgatgcc aatatcatta
    10921 gcgtgtcgca gcgcgaattt gagcaaatct acccaaaacc aggttgggta gaacacgacc
    10981 caatggaaat ctgggccacc caaagctcca cgctggtaga agtgctggcg aaagccgata
    11041 tcagttccga tcaaattgca gctatcggta ttacgaacca gcgtgaaacc actattgtct
    11101 gggaaaaaga aaccggcaag cctatctata acgccattgt ctggcagtgc cgtcgtaccg
    11161 cagaaatctg cgagcattta aaacgtgacg gtttagaaga ttatatccgc agcaataccg
    11221 gtctggtgat tgacccgtac ttttctggca ccaaagtgaa gtggatcctc gaccatgtgg
    11281 aaggctctcg cgagcgtgca cgtcgtggtg aattgctgtt tggtacggtt gatacgtggc
    11341 ttatctggaa aatgactcag ggccgtgtcc atgtgaccga ttacaccaac gcctctcgta
    11401 ccatgttgtt caacatccat accctggact gggacgacaa aatgctggaa gtgctggata
    11461 ttccgcgcga gatgctgcca gaagtgcgtc gttcttccga agtatacggt cagactaaca
    11521 ttggcggcaa aggcggcacg cgtattccaa tctccgggat cgccggtgac cagcaggccg
    11581 cgctgtttgg tcagttgtgc gtgaaagaag ggatggcgaa gaacacctat ggcactggct
    11641 gctttatgct gatgaacact ggcgagaaag cggtgaaatc agaaaacggc ctgctgacca
    11701 ccatcgcctg cggcccgact ggcgaagtga actatgcgtt ggaaggtgcg gtgtttatgg
    11761 caggcgcatc cattcagtgg ctgcgcgatg agatgaagtt gattaacgac gcctacgatt
    11821 ccgaatattt cgccaccaaa gtgcaaaaca ccaatggtgt gtatgtggtt ccggcattta
    11881 ccgggctggg tgcgccgtac tgggacccgt atgcgcgcgg ggcgattttc ggtctgactc
    11941 gtggggtgaa cgctaaccac attatccgcg cgacgctgga gtctattgct tatcagacgc
    12001 gtgacgtgct ggaagcgatg caggccgact ctggtatccg tctgcacgcc ctgcgcgtgg
    12061 atggtggcgc agtagcaaac aatttcctga tgcagttcca gtccgatatt ctcggcaccc
    12121 gcgttgagcg cccggaagtg cgcgaagtca ccgcattggg tgcggcctat ctcgcaggcc
    12181 tggcggttgg cttctggcag aacctcgacg agctgcaaga gaaagcggtg attgagcgcg
    12241 agttccgtcc aggcatcgaa accactgagc gtaattaccg ttacgcaggc tggaaaaaag
    12301 cggttaaacg cgcgatggcg tgggaagaac acgacgaata atgtaaatgc cgaatgaagc
    12361 gtttatgccg catccggtag tcccgaaacg tgcgggggca accccgcaca catcaataat
    12421 ccctcccttc ccctgtgcta cacttcgcgc cattccttac tgcttagagt ttgctatgag
    12481 acgagaactt gccatcgaat tttcccgcgt caccgaatca gcggcgctgg ctggctacaa
    12541 atggttagga cgcggcgata aaaacaccgc ggacggcgcg gcggtaaacg ccatgcgtat
    12601 tatgctcaac caggtcaaca ttgacggcac catcgtcatt ggtgaaggtg aaatcgacga
    12661 agcaccgatg ctctacattg gtgaaaaagt cggtactggt cgcggcgacg cggtagatat
    12721 tgctgttgat ccgattgaag gcacgcgcat gacggcgatg ggccaggcta acgcgctggc
    12781 ggtgctggca gtaggcgata aaggctgctt cctcaatgcg ccggatatgt atatggagaa
    12841 gctgattgtc gggccgggag ccaaaggcac cattgatctg aacctgccgc tggcggataa
    12901 cctgcgcaat gtagcggcgg cgctcggcaa accgttgagc gaactgacgg taacgattct
    12961 ggctaaacca cgccacgatg ccgttatcgc tgaaatgcag caactcggcg tacgcgtatt
    13021 tgctattccg gatggcgacg ttgcggcctc aattctcacc tgtatgccag acagcgaagt
    13081 tgacgtgctg tacggtattg gtggcgcgcc ggaaggcgta gtttctgcgg cggtgatccg
    13141 cgcattagat ggcgacatga acggtcgtct gctggcgcgt catgacgtca aaggcgacaa
    13201 cgaagagaat cgtcgcattg gcgagcagga gctggcacgc tgcaaagcga tgggcatcga
    13261 agccggtaaa gtattgcgcc tgggcgatat ggcgcgcagc gataacgtca tcttctctgc
    13321 caccggtatt accaaaggcg atctgctgga aggcattagc cgcaaaggca atatcgcgac
    13381 taccgaaacg ctgctgatcc gcggcaagtc acgcaccatt cgccgcattc agtccatcca
    13441 ctatctggat cgcaaagacc cggaaatgca ggtgcacatc ctctgattga tttgatcgat
    13501 tgagccttcc agtccttcgg gactggaatt tttttgttcg gagaacgaag ataaggcaag
    13561 tcaatcaaaa caggagaaaa acatggctga ttgggtaaca ggcaaagtca ctaaagtgca
    13621 gaactggacc gacgccctgt ttagtctcac cgttcacgcc cccgtgcttc cgtttaccgc
    13681 cgggcaattc accaagcttg gccttgaaat cgacggcgaa cgcgtccagc gcgcctactc
    13741 ctatgttaac tcgcccgata atcccgatct ggagttttac ctggtcaccg tccccgatgg
    13801 caaattaagc ccacgattgg cggcactgaa accaggcgat gaagtgcagg tggttagcga
    13861 agcagctggc ttctttgttc tggatgaagt accggactgc gaaacgctat ggatgctggc
    13921 aaccggtaca gcgattggcc cttatttatc gattctgcaa ctaggcaaag atttagatcg
    13981 cttcaaaaat ctggtcctgg ttcacgccgc acgttatgcc gccgacttaa gctatttgcc
    14041 actgatgcaa gaactggaaa aacgctacga agggaaactg cgcattcaga cggtggtcag
    14101 tcgggaaacg gcagcggggt cgctcaccgg gcggataccg gcgttaattg aaagtgggga
    14161 actggaaagc gcgattggcc tgccgatgaa taaagaaacc agccatgtga tgctgtgcgg
    14221 caatccacag atggtgcgcg atacacaaca gttgctgaaa gagacccggc agatgacgaa
    14281 acatttacgt cgccgaccgg gccatatgac agcggagcat tactggtaag cggttactta
    14341 tcgataaacg gcacgatgag caaatccgca ctcatcttat tgatcatccc gcgatatgcc
    14401 ggcatcaaac ggttgataaa tgagtgatga tgaccacaga caaggaggtc gcactgctct
    14461 ttttgcataa tttccagcag tgtttccggc atttctccgc gttcaatacg cagttttgtc
    14521 ttcggccatt gaatattttt cgtcagttta tacagcttgt tatccgactt attcttcaac
    14581 aattgaagaa tatcttctgt tgcagggaag tagatacccg gatacaactc gcttaagcca
    14641 tcatcaatat gaattaacgt caggtgagcg tcattatgtc tggcgagctc cagagcttta
    14701 ttcaccagta aggcatcttc ttcattcccg gaaattgcca cgccaatgtg tttataagcc
    14761 atgtttaact ccttctaaag caactccatc aagctaacga acgcagtgat aactcaaaaa
    14821 taatcatctc ggcctggcat gagaaagtga aggccgcatc aagctcaact tgcccctcca
    14881 gatccttaat cttgtaggcc acgctggccc cttcatctgc agaggatgca gcggcggcaa
    14941 gctctttcag tttggtcata aaagactctg cttcttcgcg ggtggcaaaa aagcgcgaaa
    15001 acttactggt acagttgtcg ttatcaatca ccgtaccgat atcaatcgca catcctttag
    15061 tactgcattt atctacgaca tctttcatag ggcacctctg tatattcgcc tctctgtcga
    15121 ttacgactta agaataatgc gccgttaagg gaagaacaaa caagtaatga tcttatggat
    15181 taccattttt tttgatattg cttaaggtga agagttcaga aggattttcc gggtattgtc
    15241 atttgcatcg tggaaataac aatcccggaa aaaaatagca gtaagagata tattaaaggt
    15301 taattacttg atttattgtc ggctttatac ttcacatcct gagtatcttt accatattta
    15361 ttttcgcctt gtgtgccaac aaacgcacca agatcgataa gcatcatcac cagaatcaac
    15421 gtcgggacaa aacgccccac cgcccattgc cagacacccg gtaaaatcgc ccagttaccc
    15481 gccagcagca tccacgccac aatcatcaga aatgcccatg cgccggaacg cccgcgatca
    15541 tgcaagcgct taacagttac tgccgctgtt ggccaaagca agcacacaag gcaaaacgcc
    15601 gcggtctgaa tatcgagtaa atttttaccc gccagtgaga aaagcaccag catgcctgcg
    15661 aaccacaggc ctatccaaat ccagaaatca cggcgtccaa tacgcccttt aaatgagaat
    15721 aaccattgct gtatggtcat gtaagttcct tgatggttgt cttttccagg attctaccct
    15781 tttgacaagg gcgacagata tcgttttaat cggagccagt cataacaaaa ggtactgtca
    15841 atgaagccag ggtgtacgct gttttttctc ttatgttctg cattaaccgt tacaacaacg
    15901 gcgcatgcac aaacaccaga tacggcaacg accgcgcctt atctgctggc tggagcccct
    15961 actttcgatc tctccatcag ccagtttcga gaagacttta acagccagaa tcctagcctg
    16021 ccactgaacg aatttcgtgc catcgacagc agtcccgaca aagccaatct cactcgtgct
    16081 gcaagtaaaa ttaacgagaa cttgtatgct tctacagcgc tggagcgcgg taccttaaaa
    16141 atcaaaagca ttcaaatgac ctggctaccc atccaggggc cagagcaaaa agcagcgaaa
    16201 gcgaaagctc aggaatacat ggcagcagtg atccgcacac tcaccccatt aatgaccaaa
    16261 acacaaagcc agaaaaaact gcagtcgctg ctaacggcgg ggaaaaacaa acgttattac
    16321 accgagacag aaggtgcact gcgttatgtt gtcgcggaca acggcgaaaa ggggctgacc
    16381 ttcgctgttg aaccgattaa gctggcgcta tctgaatcgc ttgaaggttt gaataaatga
    16441 caaaaagcaa agcctttgtg ccgatgaatc tctatactgt ttcacagacc tgctgccctg
    16501 cggggcggcc atcttccttt attcgcttat aagcgtggag aattaaaatg cgacatcctt
    16561 tagtgatggg taactggaaa ctgaacggca gccgccacat ggttcacgag ctggtttcta
    16621 acctgcgtaa agagctggca ggtgttgctg gctgtgcggt tgcaatcgca ccaccggaaa
    16681 tgtacatcga tatggcgaag cgcgaagctg aaggcagcca catcatgctg ggtgcgcaaa
    16741 acgtggacct gaacctgtcc ggcgcattca ccggtgaaac ttctgccgct atgctgaaag
    16801 acatcggcgc tcagtacatc atcatcggcc actctgaacg tcgtacttac cacaaagagt
    16861 ctgacgaact gatcgcgaaa aaattcgcgg tgctgaaaga gcagggcctg actccggttc
    16921 tgtgcatcgg tgaaaccgaa gctgaaaacg aagcgggcaa aactgaagaa gtttgcgcac
    16981 gtcagatcga cgcggtactg aaaactcagg gtgctgcggc attcgaaggt gcggttatcg
    17041 cttacgaacc tgtatgggca atcggtactg gcaaatctgc aactccggct caggcacagg
    17101 ctgttcacaa attcatccgt gaccacatcg ctaaagttga cgctaacatc gctgaacaag
    17161 tgatcattca gtacggcggc tctgtaaacg cgtctaacgc tgcagaactg tttgctcagc
    17221 cagatatcga cggcgcgctg gttggcggtg cttctctgaa agctgacgct ttcgcagtga
    17281 tcgttaaagc tgcagaagcg gctaaacagg cttaagtctg acaggtgccg gatttcatat
    17341 ccggcactta ctttccttaa ctcctcgcct taacgcaaaa tctcacactc atgatcctga
    17401 atttcctcgg ctgaagcacg gttaagcgtc agtagatttc gttgtgtcgc cagcaataca
    17461 aatgagttat cactctgccg taccatcgcc agcccgtagc ttcccatatg ttcccgcgcc
    17521 tcaggtactt cttctgccag catcataaat gggctgcgtt gtaccagttc gctttccgtt
    17581 acccgacgcg ccaggtattc atgcccgcgc aaaccacctg gcagtggcaa ccagcggctg
    17641 ctgatgttcg ccagattgtt atccagttgt tcgcgcacat caggacgaat acaagagata
    17701 tgaatatgaa aatggttttg cgtacgcccg gtgcgggagt tgatcgccaa agaaaccgcg
    17761 cgatcgggaa ccggctggcc gtattttttg ctcataaaat cacgcgcctg ccaggccaac
    17821 caaaagaagt tcggcgttga aggatcggtc aacaaaggac tttcagtacc gttaatacga
    17881 tacgttggca tcaacagata ttgcagtgga ccattaagat cttttaaaac cacgtatccg
    17941 gcattgggtt tgacttccgc acatggcgaa ggattttgat tttgctgctg attgggcaaa
    18001 cattcctcaa ggacaatctt acgtaatgta tccgactctt caccggttaa tttccagtaa
    18061 ccaataccgg cagccacaac ggcgataact atcatcacca aaaaaagaag acccgctttt
    18121 ttcatctttt tttccctgta cctcaaagag atgcaagggt aacgcaaaat cgtgacaaat
    18181 aaaaaacccg gtcgcgagaa accaccgggt ttgataatta tcctggggaa atcagcgttt
    18241 gctgatctga tcgaacgtac cgccgttagc aaaatgctct ttttgcgctt tcgtccagcc
    18301 gccgaactct tcatcaatgg tgaataactt cagctttgga aacgcatttt cgtacttttt
    18361 cgccacctca gcgtcgcgcg gacggtagta gtttttcgcg gcaatttcct gaccttctgg
    18421 cgagtagaga tatttcaggt aggcttccgc cacctctttg gtgccttttt tctcgaccac
    18481 tttatcgacc accgacacgg ttggctctgc gaggatagac tcactcggcg tgacgatttc
    18541 gaatttatct ttccccagtt cattcgctgc cagcagagct tcgttttccc aggcgatcag
    18601 tacatcgcca atcccgcgct cgacaaaggt gttagtggag ccgcgcgcgc cagaatccag
    18661 aacttcgacg tttttataca gtgcccgaac aaaatcctgt gcttttgcct gatcgttgtt
    18721 gttgtgatgc agcgcgtagc cccaggcagc caggtagttc cagcgtgcgc caccagagct
    18781 tttcggatta ggcgtgatca ccgaaacacc cggtttaatc agatcgttcc agtcatggat
    18841 ctgcttcgga ttgcccttac gcaccaggaa aacaatggtg gaagtgtacg gtgcggagtt
    18901 atccggcaga cgtttgatcc actctttatc aatccgcccg cgttccgcaa ttgcgtccac
    18961 gtcataggcc agagccagcg tgacaacatc agcttcaata ccgttgatta ccgacgtcgc
    19021 ttgtttacct gaaccaccgt gtgactgacg aatcaccacg ttatcaccag tttgctgttt
    19081 ccagtgggcg ctgaatgcct tgttgtactg ttcgtacaat tcgcgcgttg gatcatatga
    19141 aacgttaaga agctgaatat cctttgccat aacgctggtt gccgccagca aaaatgttaa
    19201 ccctacgccc cacttgttca tcgcccgact ctcttatgtt gtgttgtgat gagcaaagcg
    19261 tgccagaagg ttaaccaaac attaaagaat aaaaaaagat tggctataac ttgcgggtat
    19321 atgttgaagg attaaaaagg cggaagaaat ccgcctcata ttgctgacaa agtccgcttt
    19381 gtccatgccg gatgcggcgc gaacgcctta tccggcctac aaaagtttgc aaattcaata
    19441 aattgcagaa ttaatgtagg cctgataagc gaagcgcatc aggcattttt gcttctgtca
    19501 tcggtttcag gctaaatgaa tctgcctttt tccgaaatca ttaatacagt tttttcgcgc
    19561 agtccagcca gtcgcctttg aacggacgct tcatgttttc gatagcgtcg atgatgtcgt
    19621 ggtgaaccaa ctgttcgttc tggataccta cgcaacgacc gccgtaacct gccagcagca
    19681 gatcgatagc gtaagcgccc atacgggaag ccagaatacg gtcgtaaggc accggagaac
    19741 caccgcgctg gatgtggccc agcacagttg cgcgggtttc acgaccggtt tctttctcga
    19801 tgaaatgcgc cagttcgtca acatcacaca tatgttcggt aatcgccacg atcgcgtgtt
    19861 ttttaccttt cgcgataccc gctttgattt cgtttaccag gtcttcacgg ctgaattcaa
    19921 cttccggaac cacaacgaat tcacagccac cggcaatggc cgcagccagg gtcagatcgc
    19981 cacaataacg gcccatcact tccaccacgg aaatacgctg gtgagaagaa gaggtgtcac
    20041 gcagacggtc gatcgcttct acaacggtgc tcagcgcagt gaagaaaccg atagtgtagt
    20101 cagtgccttt gatgtcgttg tcgatagtgc ccggcagacc gatgcacggg aagcccattt
    20161 cggtcagacg cattgcaccc atgtaggaac cgtcaccgcc gataaccacc agcgcgtcga
    20221 taccacgttt tttcaggttt tcgatagcca cggcgcggat gttctcgtcg cggaattccg
    20281 ggaaacgcgc agaaccgagg aacgtaccgc cacggttgat catgtcagac acgctgtaac
    20341 ggtctagctg taccatacgg tcttcataca gacccagata gccgtcataa atacccatta
    20401 cttccagacc ttctgtcagc gcagaacgaa caaccccgcg aattgcggcg ttcatgcctg
    20461 gcgcatcacc gccgcttgtc aacacaccga ttttcttaat catgactacc tctgaacttt
    20521 ggaatgcaaa atgaaatctg ttgccggaag tcttcttgca catcgaagtg atccaacgaa
    20581 tgtgcaaata gtataacaat cacttcctgc tgaattgatt caggtcaggc caaatggcgg
    20641 tattttatac acaaaatgcg ggtctggctc tcttttatac tgattatgaa agcatagacc
    20701 gtttaccctc cctgggtacg acggaacagg ggtcctgatg gataattaca tccgatcccg
    20761 gaaaacgccg taaaatagcc tgctctacct gatccgccac catatgtgcc tgaaccaaag
    20821 gcagagagtc ttccatttcc aaatgaatct gaataaagcg ggtcggccct gactgccgcg
    20881 tgcgaagatc gtgagcgccg ctaacacccg gccaggaagt cacgatatca ataatttctt
    20941 gccgttcctc atcaggcaat gcgcgatcca gtaatgactg taccgcctca tatcccatgc
    21001 gtaacgcgct atataaaata tagatgccga ttcccaatgc aaacagagca tcggcgcgat
    21061 gccagccgta ccaggacaac cccagcgcca gcagaattgc gccgttcatc ataacatcag
    21121 actggtaatg tagcatatca gcccgcaccg cctggctttg cgtccgccgc accacccaac
    21181 gctgaaacga gacaaggata atcgtacaaa ttagcgccac aattgtcacg ataaccccga
    21241 cgcctggatc tgtcatcagt gttggagata tcagatgttg aatacccgtc aaaaacagga
    21301 atagtgccga accggagata aacatacttt gcgccagcgc cgcgagggac tcagctttac
    21361 cgtgaccaaa cgagtgatta tcgtcggcag gttgcaggga atatcgcacc accaataaat
    21421 tcgtcaacga cgcgccgata tccaccagcg aatccaccag cgcggcgaga atactcaccg
    21481 acccggtata ccaccatgca aaaattttaa tcagcaatag cagcgaagcc atcgccgtcg
    21541 cagcaatcgc cgcccgactg accagccgtc cataagattg attcataaat actcccgcta
    21601 tcaactgacg ctagtataac ggaagcaaat catctgcaat gcattaagca gcaggcaaat
    21661 tgaggataaa aaaaaccccc acatcatgtg ggggaagaca gggatggtgt ctatggcaag
    21721 gaaaacaggg tttactactg ggaacgtgag ttgctactac tcaatagctt caacgatgaa
    21781 cttttttgcc attgcgtcac gtcacgcaac tgctccattc gttgttgatg tttctcgttt
    21841 aaaaccgctt gctgctccgg cgttaacagg cgatacattt ggttgcggac ttttgccatc
    21901 tcaacctgac gagcaatttg ctcattcgcc attttttctg cctgtgcgcg cacagcgttt
    21961 tcatcaaaat tttctgcggt gacaaggcga tgcattgtct ccagttcgct aacattaaca
    22021 ggaggctgtt cgtgccgggc ctgttgcata agatctcgca tctgctgacg ctgatgttcg
    22081 gttaaactta tgccgtcgaa catatggctc tgcgtactgc gctgcgtaag ttcttcaccc
    22141 ggatgccagt tatcgcctga accgacttca gcagcgtggc ttaatgaact gactgccagc
    22201 gttgaggcca tgacggcagc ggtaactatg cgcatcattt gctcccaaaa tctttctgtc
    22261 gcgattcaac gatagagagt ttacgattca ggctgcaaac atgcgtcagg gggtgtaaaa
    22321 caacgtaaag tcatggatta gcgacgtctg atgacgtaat ttctgcctcg gaggtattta
    22381 aacaatgaat aaaatcctgt tagttgatga tgaccgagag ctgacttccc tattaaagga
    22441 gctgctcgag atggaaggct tcaacgtgat tgttgcccac gatggggaac aggcgcttga
    22501 tcttctggac gacagcattg atttactttt gcttgacgta atgatgccga agaaaaatgg
    22561 tatcgacaca ttaaaagcac ttcgccagac acaccagacg cctgtcatta tgttgacggc
    22621 gcgcggcagt gaacttgatc gcgttctcgg ccttgagctg ggcgcagatg actatctccc
    22681 gaaaccgttt aatgatcgtg agctggtggc acgtattcgc gcgatcctgc gccgttcgca
    22741 ctggagcgag caacagcaaa acaacgacaa cggttcaccg acactggaag ttgatgcctt
    22801 agtgctgaat ccaggccgtc aggaagccag cttcgacggg caaacgctgg agttaaccgg
    22861 cactgagttt accctgctct atttgctggc acagcatctg ggtcaggtgg tttcccgtga
    22921 acatttaagc caggaagtgc tgggcaaacg cctgacgcct ttcgaccgcg ctatcgatat
    22981 gcacatttcc aacctgcgtc gtaaactgcc ggatcgtaaa gatggtcacc cgtggtttaa
    23041 aaccttgcgt ggtcgcggct atctgatggt ttctgcttca tgataggcag cttaaccgcg
    23101 cgcatcttcg ccatcttctg gctgacgctg gcgctggtgt tgatgttggt tttgatgtta
    23161 cccaagctcg attcacgcca gatgaccgag cttctggata gcgaacagcg tcaggggctg
    23221 atgattgagc agcatgttga agcggaactg gcgaacgatc cgcccaacga tttaatgtgg
    23281 tggcgacgtc tgttccgggc gattgataag tgggcaccgc caggacagcg tttgttattg
    23341 gtgaccaccg aaggccgcgt gatcggcgct gaacgcagcg aaatgcagat cattcgtaac
    23401 tttattggtc aggccgataa cgccgatcat ccgcagaaga aaaagtatgg ccgcgtggaa
    23461 ctggtcggtc cgttctccgt gcgtgatggc gaagataatt accaacttta tctgattcgt
    23521 ccggccagca gttctcaatc cgatttcatt aacttactgt ttgaccgccc gctattactg
    23581 ctgattgtca ccatgttggt cagtacgccg ctgctgttgt ggttggcctg gagtctggca
    23641 aaaccggcgc gtaagctgaa aaacgctgcc gatgaagttg cccagggaaa cttacgccag
    23701 cacccggaac ttgaagcggg gccacaggaa ttccttgccg ctggtgccag ttttaaccag
    23761 atggtcaccg cgctggagcg catgatgacc tctcagcagc gtctgctttc tgatatctct
    23821 cacgagctgc gcaccccgct gacgcgtctg caactgggta cggcgttact gcgccgtcgt
    23881 agtggtgaaa gcaaggaact ggagcgtatt gaaaccgaag cgcaacgtct ggacagcatg
    23941 atcaacgatc tgttggtgat gtcccgtaat cagcaaaaaa acgcgctggt tagcgaaacc
    24001 atcaaagcca accagttgtg gagtgaagtg ctggataacg cggcgttcga agccgagcag
    24061 atgggcaagt cgttgacggt taacttcccg cctgggccgt ggccgctgta cggcaatccg
    24121 aacgccctgg aaagtgcgct ggaaaatatt gttcgtaatg ctctgcgtta ttcccatacg
    24181 aagattgaag tgggctttgc ggtagataaa gacggtatca ccattacggt ggacgacgat
    24241 ggtcctggcg ttagcccgga agatcgcgaa cagattttcc gtccgttcta tcggaccgat
    24301 gaagcacgcg atcgtgaatc tggcggtaca ggattggggc tggcgattgt tgaaaccgcc
    24361 attcagcagc atcgtggctg ggtgaaggca gaagacagcc cgctgggcgg tttacggctg
    24421 gtgatttggt tgccgctgta taagcggagt taaactccgc atttgtaggc aggataaggc
    24481 gtttacgccg catccggcat ttgagcagga tgcctgatgc gacgctgata gcgtcttatc
    24541 aggcctacac tccgggggtt attttcccca caaccggcgg gaaaaatctt cgatcttgcc
    24601 gctcagtcgg cgcttttgca tcgtccgcgt ccagcttttc gataacccgg cggcggagag
    24661 taaacggtga tattgatcgt catcaaacgg catatgccat gcgatggcgg cagcctcctg
    24721 cacggtcaca tcgctgacac gggaaaccaa ctccagcggc gcgtccgcag acactttccc
    24781 tggcgctatc acgctataca accaccccac cttacctgtg ttttgcatca actgcgctat
    24841 atcgctgata tcaaaatggt aattgagttt atagcaaggc gagcgcggct ggctgacctg
    24901 aattaatgcc tctccccagc ggaaaatatc gcccatataa acattacttt ccgtcaggcc
    24961 gtcggttgag aggttttcac caaacgcagg cgcaacaaac aactccgcct gttccggaaa
    25021 ttcccgcgcc cagtagagat aatgctcacg aggataatga cacagcgctc tgtctggccc
    25081 gccgtgaacc tttttctccg cctgctcgtc accttccagc cccagctccg tcagcatcaa
    25141 ctcaccatca acctggattt tggcaattgc gctgggtttg ccttcgggat aagcctgaat
    25201 cttgcctgtg tatacatcaa ccggatatcg catctgttcc ctctccacga gaaatttccc
    25261 cattagacca tatttatttt atgtcaggcg tgctgttttc gcggtaagta tccaggcagg
    25321 aactcaatag caaaccggac gaacgttagt tcatccggct gtggggatta aggaagcagg
    25381 tttatttcac cgtacctaaa atttcgattt tcgctgctct ggctttgact ttacgtgacc
    25441 agatagaggt aagaattggc accagaatcg aagtcacaat gaccgccgtc gctaccagcg
    25501 aagttgctgc cggagccatc ggtttaaacg caggcaccat ttctgcaatc agcacaggtg
    25561 tcgctaccgc ggcccctgcg gaactggaag cggcaattcc ggcagtgcca tcgccaccgc
    25621 caatcaattt atcggcgata atcaacggaa taccggtcac gataattact gccacaccca
    25681 acaggatccc cagcaaacca gtctgggcaa tcacagtcaa atcaatggta ttgcccagcg
    25741 cgaaggcaaa gaatggaatc agcgtttgca ccgctttgct gaaaaattct cgtaattcag
    25801 ggtcaaggtt ccccagggca aagcccacca ggaacggtaa tactgcgccg acgaaaacat
    25861 gcggttcaaa cgaggcaatc cctgcagtgc ccagaataat catcgtcatg agcggcccgg
    25921 actccaacga catcaacaca aatgccccag cttcttcttt tgtgccgtac tgctgcatga
    25981 tggaagcgta aagtccgcca ttggtcatat ccatcgccgc caccagcgcc agcgttgaaa
    26041 gtccggcaaa gaatccaact tcaacaccat gttccggaat aatgcgcgag gcaattgccg
    26101 caaccaccca cgcgacggca atttttgtta ccaccagcgt accggattta cgcaataccg
    26161 ttcccgtcgc gcttaatttt attgacgccc ccatgcaaaa aaaccacacc gccagaatgg
    26221 gcaccgtacc ggtaatcatc ccgttggtga atgatccaaa atatttcccc gcccccggcg
    26281 agaaggtgtg gcacagtgcg ccaaggaata gcgggacgag catcatcccc cccgagattt
    26341 tctcaatcga gcgttttatc tgcatttcca tcacctgtcg gcatattttt aagggctgat
    26401 gcgtcattcc tcaatggagc atcagtgttg atggaaacga agata
//
LOCUS       DAZGVQ010000064        25997 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000064 DAZGVQ010000000
VERSION     DAZGVQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.064"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(212..2785)
                     /gene="clpB"
                     /locus_tag="ACHLAG_002525"
     CDS             complement(212..2785)
                     /gene="clpB"
                     /locus_tag="ACHLAG_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFP0655733.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2915..3646)
                     /gene="yfiH"
                     /locus_tag="ACHLAG_002526"
     CDS             complement(2915..3646)
                     /gene="yfiH"
                     /locus_tag="ACHLAG_002526"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFP0655734.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="ACHLAG_002527"
     CDS             complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="ACHLAG_002527"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFP0655735.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCNKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEENKDEVDWL"
     gene            4758..5495
                     /gene="bamD"
                     /locus_tag="ACHLAG_002528"
     CDS             4758..5495
                     /gene="bamD"
                     /locus_tag="ACHLAG_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFP0655736.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5766..6107
                     /gene="raiA"
                     /locus_tag="ACHLAG_002529"
     CDS             5766..6107
                     /gene="raiA"
                     /locus_tag="ACHLAG_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFP0655737.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6210..6335
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6211..6258
                     /gene="pheL"
                     /locus_tag="ACHLAG_002530"
     CDS             6211..6258
                     /gene="pheL"
                     /locus_tag="ACHLAG_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFP0655738.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6357..7517
                     /gene="pheA"
                     /locus_tag="ACHLAG_002531"
     CDS             6357..7517
                     /gene="pheA"
                     /locus_tag="ACHLAG_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFP0655739.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="ACHLAG_002532"
     CDS             complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="ACHLAG_002532"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFP0655740.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="ACHLAG_002533"
     CDS             complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="ACHLAG_002533"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFP0655741.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9972..10337
                     /gene="yfiL"
                     /locus_tag="ACHLAG_002534"
     CDS             9972..10337
                     /gene="yfiL"
                     /locus_tag="ACHLAG_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFP0655742.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGTDENASTIRLN"
     gene            10487..11005
                     /gene="yfiR"
                     /locus_tag="ACHLAG_002535"
     CDS             10487..11005
                     /gene="yfiR"
                     /locus_tag="ACHLAG_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFP0655743.1"
                     /translation="MRFSHRLFLLLILLLTRAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSHFSNALQENGTTSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            10995..12221
                     /gene="dgcN"
                     /locus_tag="ACHLAG_002536"
     CDS             10995..12221
                     /gene="dgcN"
                     /locus_tag="ACHLAG_002536"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFP0655744.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            12237..12719
                     /gene="yfiB"
                     /locus_tag="ACHLAG_002537"
     CDS             12237..12719
                     /gene="yfiB"
                     /locus_tag="ACHLAG_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP0655745.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12796..13143)
                     /gene="rplS"
                     /locus_tag="ACHLAG_002538"
     CDS             complement(12796..13143)
                     /gene="rplS"
                     /locus_tag="ACHLAG_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFP0655746.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13185..13952)
                     /gene="trmD"
                     /locus_tag="ACHLAG_002539"
     CDS             complement(13185..13952)
                     /gene="trmD"
                     /locus_tag="ACHLAG_002539"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFP0655747.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13983..14531)
                     /gene="rimM"
                     /locus_tag="ACHLAG_002540"
     CDS             complement(13983..14531)
                     /gene="rimM"
                     /locus_tag="ACHLAG_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFP0655748.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14550..14798)
                     /gene="rpsP"
                     /locus_tag="ACHLAG_002541"
     CDS             complement(14550..14798)
                     /gene="rpsP"
                     /locus_tag="ACHLAG_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFP0655749.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15047..16408)
                     /gene="ffh"
                     /locus_tag="ACHLAG_002542"
     CDS             complement(15047..16408)
                     /gene="ffh"
                     /locus_tag="ACHLAG_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFP0655750.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16575..17366
                     /gene="ypjD"
                     /locus_tag="ACHLAG_002543"
     CDS             16575..17366
                     /gene="ypjD"
                     /locus_tag="ACHLAG_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFP0655751.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17387..18673
                     /gene="yfjD"
                     /locus_tag="ACHLAG_002544"
     CDS             17387..18673
                     /gene="yfjD"
                     /locus_tag="ACHLAG_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFP0655752.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18728..19321)
                     /gene="grpE"
                     /locus_tag="ACHLAG_002545"
     CDS             complement(18728..19321)
                     /gene="grpE"
                     /locus_tag="ACHLAG_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFP0655753.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19444..20322
                     /gene="nadK"
                     /locus_tag="ACHLAG_002546"
     CDS             19444..20322
                     /gene="nadK"
                     /locus_tag="ACHLAG_002546"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFP0655754.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20408..22069
                     /gene="recN"
                     /locus_tag="ACHLAG_002547"
     CDS             20408..22069
                     /gene="recN"
                     /locus_tag="ACHLAG_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFP0655755.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22218..22559
                     /gene="bamE"
                     /locus_tag="ACHLAG_002548"
     CDS             22218..22559
                     /gene="bamE"
                     /locus_tag="ACHLAG_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFP0655756.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22621..22911)
                     /gene="yfjF"
                     /locus_tag="ACHLAG_002549"
     CDS             complement(22621..22911)
                     /gene="yfjF"
                     /locus_tag="ACHLAG_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFP0655757.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22901..23338)
                     /gene="ratA"
                     /locus_tag="ACHLAG_002550"
     CDS             complement(22901..23338)
                     /gene="ratA"
                     /locus_tag="ACHLAG_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFP0655758.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23509..23991
                     /gene="smpB"
                     /locus_tag="ACHLAG_002551"
     CDS             23509..23991
                     /gene="smpB"
                     /locus_tag="ACHLAG_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFP0655759.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24206..24568
                     /gene="ssrA"
                     /locus_tag="ACHLAG_002552"
     tmRNA           24206..24568
                     /gene="ssrA"
                     /locus_tag="ACHLAG_002552"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(24729..25997)
                     /locus_tag="ACHLAG_002553"
                     /pseudo
     CDS             complement(24729..25997)
                     /locus_tag="ACHLAG_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
BASE COUNT         6546 a         6917 c         6120 g         6414 t
ORIGIN      
        1 cggaattctc aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagcga aaaatataaa aactttatcg
      121 ttcgctcatt tttcaggcaa aacatcttaa atatagtctt ttccgtctaa cttatagaca
      181 aaaacgagcc ccgaagggct cgttttatca tttactggac ggcgacaatc cggtcttcat
      241 taacttccag gcgaatcact ttacccggaa ccaattcacc agacagtatt tgctgtgcca
      301 gcgggttttc gatctgctgc tggatagcac gtttcaaagg acgtgcacca tagactggat
      361 cgtaaccgtt ctcgctcagc agtttcagcg cctcgtcaga aatgtggatt tcataaccac
      421 gttcttccag acgtttgtac agacgtttca actgaatctg cgcaatcgag gcaatgtgct
      481 gttcacccag cggatggaag accaccactt catcaatacg gttaatgaat tccggacgga
      541 agttatggct taccacaccg agcaccagct ctttcatgtg cgcataatcc agttcaccga
      601 agcgttcctg aatcaaatcg gaaccgaggt tagaggtcat aatgacaacc gtattacgga
      661 agtcgaccgt tctcccctgc ccgtcagtca gacgaccatc atccagtacc tgcaacagaa
      721 tgttgaagac atccggatgc gctttttcca cttcatccag caggatgacg gaatacggac
      781 gacgacgcac agcttcggtc aggtagccac cttcttcata accgacatat cccggaggcg
      841 caccaaccag acgagacacc gagtgtttct ccataaactc ggacatatcg atacggacca
      901 tcgcctcgtc gctatcaaac ataaagttcg ccagcgcctt acaaagctca gttttcccca
      961 cacccgttgg gccgaggaac aggaatgaac caatcgggcg atttggatcc gccaaccccg
     1021 cacggctacg gcgaatagcg ttagataccg catcaaccgc ttcgttctga ccaatgacgc
     1081 gatggtgcag ttcttgctcc atacgcagca gtttttcgcg ctcgctttcc atcatgcgag
     1141 aaaccggaat ccccgtccaa cgcgccagca cttcagcaat ttcggcgtcg gtcactttat
     1201 tacgcaacag acgcatagtt ttgccttcga gctgcgttgc ggcttccagt tgcttttcca
     1261 gttccgggat tttgccgtat tgcagttcag acatccgcgc caggtccccc acacggcgag
     1321 cctgttcaat agcgattttc gcctgttcca gttccgcttt aatggtctgc gtaccagaaa
     1381 gcgatgcctt ctctgctttc cactcttctt ctaactcgga gtactgacgt tctttgtcgc
     1441 tcagttcttc gttgagcata tccaggcgtt ttttactggc ttcatcagac tctttcatta
     1501 acgcctgttg ttccagtttg agctggatga tacgacgatc gagtcggtcg agttcttctg
     1561 gttttgagtc aatctgcata cgaatgctgg atgctgcttc atcgatcagg tcgatggctt
     1621 tatccggcag ctgacggtca gcaatgtagc gatgagacaa cgtcgccgct gcaacaattg
     1681 ccgggtcagt aatttgcaca tggtggtgca attcgtaacg ttctttcagg ccacgcagaa
     1741 tcgcaatggt atcttcaaca gaaggctcgg caacaaacac tttctggaaa cgacgttcca
     1801 gcgcagcatc tttttcgatg tactggcgat attcgtcaag cgtcgtggca cctacgcagt
     1861 gcaattcacc acgcgccagc gccggtttca gcatgtttcc ggcgtccatt gcgccatcgg
     1921 ctttacccgc gccgaccatg gtatgtaatt cgtcgataaa caggatgacg ttgccttcct
     1981 gtttggcaag atcgttaagc acgcctttta aacgttcttc aaactcaccg cgatatttcg
     2041 ccccagccac cagcgcgccc atatccagcg ccagtacgcg gcggcctttc aatccttccg
     2101 gcacttcgcc gttgataata cgctgcgcca gaccttcaac gatggcagtt ttaccgacgc
     2161 cgggttcacc aatcagtacc gggttatttt tagtacgacg ttgcagcacc tgaatggtac
     2221 ggcgaatttc ttcatcacga ccaatcaccg gatcgagttt gccctgttcg gctcgttcgg
     2281 taaggtcgat ggtatatttt ttcaaagcct gacgttggtc ttcagcacct tgatcgttca
     2341 cgctttcacc tccacgcatt tgttcaatcg cttgagtaat gttggcggtg gtcgcccctg
     2401 ctgctttcag gatgtcggcc agcgtgccgc gagactcaag tgccgccaga acgaacagtt
     2461 ctgacgagat aaagttatca ccacgttttt gcgccagctt gtcgcaaaga ttaagaacgc
     2521 gcaccagatc ctgtgatggc tggacatcac caccagtacc ttcaacctgc ggtaaacgat
     2581 ttaatgcctg attgatatct gtgcgcaact ggccagcatt tatgccagcg gatgttaata
     2641 aaggactaac cgaaccccct tcctgattca gcagggcgct cattaaatga agtggttcga
     2701 taaattggtt gtcgtgcccg agtgcaagtg attgggcatc ggcaagagca agctggaatt
     2761 tattagtaag acgatccaga cgcataactc ctcccataac ggatcaaagt tgctactgga
     2821 gattaaatga ggtcatccct caattattca aggttattga ccagattaat gtgaaaagaa
     2881 aatcacgcgt accggatcgt cttgattctt taggttatat cagccaaatg aaacttgcca
     2941 tacgaccggt ggtcttgtcg cgacgataag agaagaaagt ctcattttcc gtatatgtac
     3001 aacggtcgcc gccgaaaatt tgctcaacac ccacgttcgc cagacgctgc cgggcaagct
     3061 gataaatatc cgccagatac ttatcaccat gctgaatgaa agctgcactt gctttagcgt
     3121 ctactgccat aaacgcctcg cgaacctcca cccccacttc gaacgcgcgt ggaccaattg
     3181 ccggccctaa ccaggcgaga atattttccg gattatcagc aaaacaggaa accgtctctt
     3241 ccagcacgcc tgcgcacagt ccacgccagc cagcatgagc ggcggcgact tccgttcccg
     3301 ctcgattgca aaacagcaca gggaggcagt cggcagtcat cactgcgcaa accgtgccgg
     3361 gcgtattgct ataagaggca tctgctcgtt ttgaggcata aggttcgcca gtaagcttaa
     3421 gcacatcttt gccgtgtacc tgctcaagcc agaccggttt agaaggcaaa ttgcccgcag
     3481 caaaaagtcg cttgcgattc tcctcaacgt gatccgggtt atcgccacaa tgggcaccga
     3541 ggttgagcga gtcatacggg ggcaagctca cgccgccgat acgagaagag ctacaggccg
     3601 caacaccttt tggtagcggc cactgcggga caatcagctt actcataacc agtccacttc
     3661 atccttattc tcttcgaaat cggcgcgcat cacctcaatc agctccacca tatcttgtgg
     3721 aataggcgca tgccattcca tttcgatgcc ggagatcggg tgataaagac gcagcatggt
     3781 tgcatgaagc gcctggcggt caaacttacg cagcatggag ataaatgctt ccgaagcacc
     3841 ttttggcgga cgcggacggc caccataaac cggatcgccc accagcggat gagtgatatg
     3901 ggccatatgc acgcggatct ggtgcgtacg tccagtttcc agacgcaacc gcagacgcgt
     3961 gtgcacacgg aagtgttcca tgatgcgata gtgagtcacc gccggtttgc ccatcggatg
     4021 caccgccatg tgggtacgtt tggtcgggtg gcggctgatg ggttcatcta ctgtaccgcc
     4081 agcggtcata tgaccaatcg ccaccgcttc atactcacga gtaatttcac gccgttgcaa
     4141 agattcgacc aaacgcgtct gagccggaac ggtttttgcc acaaccatca ggccagtggt
     4201 gtctttatcc agacgatgga cgatgcccgc acgtggtaca tcggcaatgg gcggatagta
     4261 atgaagcaac gcattcagta ccgtgccatc cgggttaccc gcgccaggat gtaccaccag
     4321 gtcgcgcggt ttattaatga cgatgatatc ttcatcttca tagacgatat ccagcgggat
     4381 atcctgcggt tcaaaacgcg cttcttcttc aatctcagcg ttgatggcaa cctgctcgcc
     4441 acccaatact ttttctttcg gcttattaca aactttgccg tttaccagca cgcgctggtc
     4501 gaggatccat tcttttattc gcgaacgtga ataatccggg aacatttcgg ccaaagcctg
     4561 atctaagcgt tgaccgagtt ggttttcgga caccgttgca gtgagctgta ctcgttgtgc
     4621 catatatact gcttcttcgt ttatcgttgg ggttttacgg ctttgccgtt taatatagtg
     4681 tgctattgta gctggtctta accgggagca ggaacagaga atctcccgta ttacattttg
     4741 aggaaagtca aaacgtcatg acgcgcatga aatatctggt ggcagccgcc acactaagcc
     4801 tgtttttggc gggttgctcg gggtcaaagg aagaagtacc tgataatccg ccaaatgaaa
     4861 tttacgcgac tgcacaacaa aagctgcagg acggtaactg gagacaggca ataacgcaac
     4921 tggaagcgtt agataatcgc tatccgtttg gtccgtattc gcagcaggtg cagctggatc
     4981 tcatctacgc ctactataaa aacgccgatt tgccgttagc gcaggctgcc atcgatcgtt
     5041 ttattcgcct taacccgacc catccgaata tcgattatgt catgtacatg cgtggcctga
     5101 ccaatatggc gctggatgac agtgcgctgc aagggttctt tggcgttgac cgtagcgatc
     5161 gcgatcctca acatgcacga gctgcgttta gtgacttttc caaactggtg cgcggctatc
     5221 caaacagtca gtacaccacc gatgccacca aacgtctggt attcctgaaa gatcgtctgg
     5281 cgaaatatga atactccgtg gccgagtact atacagaacg tggcgcatgg gttgccgtcg
     5341 ttaaccgcgt agaaggaatg ttgcgcgact acccggatac ccaggctacg cgtgatgcgc
     5401 tgccgctgat ggaaaatgca taccgtcaga tgcagatgaa tgcgcaagct gaaaaagtag
     5461 cgaaaatcat cgccgcaaac agcagcaata cataacagaa acctgaaata caaaacggca
     5521 gcccttgagc tgccgttttt ttattctgtt agttgtgaaa ctgaagcgat ttagtcacta
     5581 tcgatctcat caaatatggc tcgctttgag atattcctca agtaaaaaaa cacctcttcc
     5641 tgcgatttct cacaaaaaag attcgttgac aaaaagtgac aaaattatga gattcccatc
     5701 acacattttg acatcaggag cggtatgctg aattcaccaa gacgggaaga caagaggtaa
     5761 aatttatgac aatgaacatt accagcaaac aaatggaaat tactccggcc atccgccaac
     5821 atgtcgcaga ccgtctcgcc aaactggaaa aatggcaaac acatctgatt aatccacata
     5881 tcattctgtc caaagagcca caagggtttg ttgctgacgc cacaatcaat acacctaacg
     5941 gcgttctggt tgccagtggt aaacatgaag atatgtacac tgcaattaac gaattgatca
     6001 acaagctgga acggcagctc aataaactgc agcacaaagg cgaagcacgt cgtgccgcaa
     6061 catcggtgaa agacgccaac ttcgtcgaag aagttgaaga agagtagtcc tttatattga
     6121 gtgtatcgcc aacgcgcctt cgggcgcgtt ttttgttgac agcgtgaaaa cagtacgggt
     6181 actgtactaa agtcacttaa ggacacaaac atgaaacaca caccgttttt cttcgcattc
     6241 ttttttacct tcccctgaat gggaggcgtt tcgtcgtgtg aaacagaatg cgaagacgaa
     6301 caataaggcc tcccaaatcg gggggccttt tttattgata acaaaaaggc aacactatga
     6361 catcggaaaa cccgttactg gcgctgcgag agaaaatcag cgcgctggat gaaaaattat
     6421 tagcattact ggcagagcgg cgcgaattgg ccgtcgaggt aggaaaagcc aaactgctct
     6481 cgcatcgccc ggtacgagat attgatcgtg aacgcgattt actggaaaga ttaattacgc
     6541 tcggtaaagc gcaccatctg gacgcccatt acattactcg cctgttccag ctcatcattg
     6601 aagattccgt attaactcag caggctttgc tccagcaaca tctcaataaa attaatccgc
     6661 actcagcacg catcgctttt ctcggcccca aaggttctta ttcccatctt gcggcgcgcc
     6721 agtatgctgc ccgtcacttt gagcaattca ttgaaagtgg ctgcgccaaa tttgccgata
     6781 tttttaatca ggtggaaacc ggccaggccg actatgccgt cgtaccgatt gaaaatacca
     6841 gctccggtgc cataaacgac gtttacgatc tgctgcaaca taccagcttg tcgattgttg
     6901 gcgagatgac gttaactatc gaccattgtt tgttggtctc cggcacgact gatttatcca
     6961 ccattaatac ggtctacagc catccgcagc cattccagca atgcagcaaa ttccttaatc
     7021 gttatcctca ctggaagatt gaatataccg aaagtacgtc tgcggcaatg gaaaaggttg
     7081 cacaggcaaa atcaccgcat gttgctgcgt tgggaagcga agctggcggc actttgtacg
     7141 gtttgcaggt actggagcgt attgaagcga atcagcaaca aaacttcacc cgatttgtgg
     7201 tgttggcacg taaagccatt aacgtgtctg accaggttcc ggcgaaaacg acgttgttaa
     7261 tggcgaccgg gcaacaagcc ggtgcgctgg ttgaagcgtt gctggtgctg cgcaaccaca
     7321 atctgattat gacccgtctg gaatcacgcc cgattcacgg taatccatgg gaagagatgt
     7381 tttatctgga tattcaggcc aatcttgaat cagcggaaat gcaaaaagca ttgaaagagt
     7441 taggggaaat tacccgttca atgaaggtat tgggctgtta cccaagtgag aacgtagtgc
     7501 ctgttgatcc aacctgatga aaaggtgccg gatgatgtga atcatccggc actggattat
     7561 tactggcggt tgtcattcgc ctgacgcaat aacacgcggc tttcactctg aaaacgctgt
     7621 gcgtaatcgc cgaaccagtg ctccaccttg cggaaactgt caataaacgc ctgcttatcg
     7681 ccctgctcca gcaactcaat cgcctcgccg aaacgcttat agtaacgttt gattaacgcc
     7741 agattacgct ctgacgacat aataatgtcg gcataaagct gcggatcctg agcaaacagt
     7801 cgcccgacca tcgccagctc aaggcggtaa atcggcgaag agagcgccag aagttgctca
     7861 agctgaacat tttcttctgc cagatgcagc ccataagcaa aagtagcaaa gtggcgcaga
     7921 gcctgaataa acgccatatt ctgatcgtgc tcgacagcgc taatacgatg cagccgagcg
     7981 ccccagacct gaatttgctc cagaaaccat tggtatgctt ccggcttacg tccatcacac
     8041 cagaccacaa cttgctttgc caggctaccg ctgtccgggc cgaacatcgg gtgtaacccc
     8101 agtaccgggc catcgtgcgc cgccagcatg gcctgtaatg gtccattttt cactgatgcc
     8161 agatcaacca gaatacaatc tttcggtaaa ggcggtaatt tgccgataac ttgctcagta
     8221 acgtggattg gcacactaac aatcaccatt ccggcatcgg caacaatatc agccgctcga
     8281 tcccagtcat gttgctccag aatccgcacc tgataacccg atagtgtcag catcttctcg
     8341 aacaggcgtc ccatctgacc gccaccgccg acgataacca ccggacgcag tgacggacaa
     8401 agtgttttaa atcctttgtc gttttcactg gagtaagatt cacgcatcac ccgacgcaaa
     8461 acatcctcaa tcagatctgg cggtacaccc agagcttccg cctctgcacg acgcgaggcc
     8521 aacatagatg cctcgcgctc cggaacataa ataggcagtc caaagcggct tttcacctcg
     8581 cccacttcag caaccagttc cagacgcttc gctaataaat tcagcagcgc tttatcgact
     8641 tcatcaattt gatcgcgtaa tgcggtcaat tcagcaacca taataaacct cttaagccac
     8701 gcgagccgtc agctgcccgt tcagatcctg atgaatttca cgcagcaagg catcggtcat
     8761 ttcccagcta atgcaggcat cggttacgga tacaccgtat ttcatttcac tgcgcggttg
     8821 ctcagaagac tgattgccct cgtggatatt actttcgatc atcagaccaa taattgagcg
     8881 attgccatct ttgatttgag caaccacgga ttctgccacc gcaggctgac ggcgataatc
     8941 tttattggaa ttaccgtggc tgcaatctac catcagagac gggcgcagtc ccgcctgttc
     9001 catctctttt tcacattgcg caacatccgc agggctatag ttcggcgctt taccaccgcg
     9061 caggatgaca tgcccgtccg gattcccctg agtttgtagc aacgcaacct gccctgcctg
     9121 gttaatgcca acaaaacggt gcggctgggc ggcggcgcgc atagcgttaa ttgctgttgc
     9181 cagactgccg tcggtgccgt ttttaaaacc aaccggcatg gaaagcccgg aggccatttc
     9241 acggtgagtt tgcgattccg ttgtacgagc accaattgct gaccagctaa acagatcgcc
     9301 caggtattgc gggctattcg gatctaacgc ttccgtcgcc agtggcagtc ccatattcac
     9361 cagctcaagc agcaatttac gcgcgatctg cagcccggct tctacatcaa aagagccatc
     9421 catatgggga tcgttaatta accctttcca gccgacagtg gtacggggtt tttcaaaata
     9481 gacgcgcatt accagataga ggctatcgct gacctctgcg gcaagggctt taaatcgacg
     9541 agcatattcc agagcagttt ccggatcatg aatggaacaa ggaccacata ctaccagcag
     9601 acgaggatcg cgcccggcga taatatctga aatggttcta cgcgagtcag caatctgggc
     9661 ttcttgttgc aggctcaatg gaaaagcggc cttcagttgt tccggagtca ttaaaacctg
     9721 ttcgtcggta atatgtacgt tattcagcgc gtctttttgc atgatggcga tcctgtttat
     9781 gctcgtttgc gatagttgat cctcagcgag gatgacgtaa cgataacaca taaagtaaag
     9841 ttttcaatcc atatttcgta catttttctt tacataagca atttattagc gttttcaacc
     9901 cttgcttctg tatagataaa tttacacccc ctttgaaaac aattcgctat gctttgaaaa
     9961 aggagaaaga aatgatgaaa aagtttatcg cccccttgtt ggctttactg gttagcggat
    10021 gccagattga tccttatact cacgcgccaa ccttgaccag caccgactgg tatgatgttg
    10081 gtatggaaga tgcgatatcg ggcagcgcca taaaagatga cgatgcattt agcgattcac
    10141 aggcggatcg cggtctatac cttaaaggat atgccgaagg acaaaagaaa acttgccaga
    10201 ccgattttac ttatgcccgg ggactttccg gtaaaagctt tcctgccagc tgtaacaatg
    10261 ttgaaaatgc cagccaactg catgaagttt ggcaaaaagg gactgatgaa aatgccagca
    10321 caatacgatt aaattaatta aaaccccaca aataaattaa acataagatt ttactcattg
    10381 tgttttattt ctcacattga tgacgatcgc atgaaaaaat gatattttaa tgagcattct
    10441 gaaaaaatat cgatgcattt cgagcgaaga tggtggggat ccctgaatgc gtttttctca
    10501 ccgactgttc ttactgctga ttctcctgct gacgagagca ccaatactcg ctcaggaacc
    10561 ttctgacgtt gcgaaaaacg tgcgtatgat ggtttccggt attgtcagtt acacccgctg
    10621 gcctgcgcta tcagggccac caaagttatg tatcttttca tcttcacatt ttagtaacgc
    10681 cctacaagag aatggcacga catctttgcc ttatctacct gtcatcattc acactcaaca
    10741 agaggcgatg atttcaggct gcaatggttt ttattttggc aacgagtcac cgacattcca
    10801 gatggaatta accgaacaat atccgtcaaa ggcgttatta ttaattgccg aacaaaatac
    10861 cgagtgcatt attggtagcg ccttttgtct gatcatccac aataatgacg tcagatttgc
    10921 cgtaaacctg gatgccttat cgcgtagcgg tgtaaaggtc aacccggatg tcttaatgct
    10981 cgcacggaag aaaaatgatg gataacgata attctcttaa taagcgcccc acgtttaaaa
    11041 gagcattacg caacatcagt atgaccagca tatttatcac tatgatgctg atctggttgc
    11101 tgctttccgt gacctcggtg ctgaccctga aacagtacgc gcaaaaaaac ctggcactga
    11161 cagcagcaac aatgacttac agtctggaag cagctgtcgt ttttgccgat ggccctgcag
    11221 caactgaaac actggcagcg ctgggccagc aagggcaatt ttcaactgca gaagtacgtg
    11281 ataagcagca aaatattctg gcatcctggc attacacccg taaggatcca ggcgatactt
    11341 tcagcaattt cataagccac tggctcttcc ctgcccccat cattcagccg attcgtcaca
    11401 atggcgaaac cattggcgaa gtacgcttaa ccgctcgcga cagttcaatc agccatttta
    11461 tctggttttc gctcgccgta ctgaccggtt gtattctgct ggcatcagga atcgcaatta
    11521 ccctcacccg ccatttgcac aatggcctgg tggaagcact gaaaaatatc accgatgtcg
    11581 tacatgatgt gcgttccaac cgcaattttt cccgacgagt ttcggaagaa cgtattgctg
    11641 agtttcaccg cttcgctctc gacttcaaca gtctgctgga tgaaatggaa gagtggcagc
    11701 ttcgtttaca ggctaaaaat gcgcagcttc tacgtaccgc gctacatgac ccattaaccg
    11761 ggctggctaa ccgcgcagcg tttcgtagcg gcatcaacac gttgatgaac aattccgatg
    11821 cccgaaaaac gtcggcgtta ctatttcttg atggcgataa tttcaaatac atcaatgata
    11881 cctggggtca tgcgacgggc gatagagtct tgattgaaat cgcaaaacgg ttagctgaag
    11941 ttggcgggct gcgacataaa gcataccgcc tgggcggcga tgaattcgct atggtgctct
    12001 atgatgtaca gtcagaatct gaagtgcagc agatatgctc agcactgaca caaatcttta
    12061 atctcccgtt tgatcttcat aatggccatc agaccaccat gacattaagc attggttacg
    12121 cgatgaccat tgagcacgcc tctgcggaaa aattacaaga gcttgccgat cacaatatgt
    12181 atcaggccaa acaccagcgt gccgaaaagc tggtgagata acaaggatat atcgatatga
    12241 taaagcacct ggtagcaccc ctgattttca cctcactaat actgactggc tgccagtccc
    12301 ctcagggaaa gtttactcct gagcaagtcg ccgctatgca atcttatgga tttactgaat
    12361 ccgccggcga ctggtcgctg ggcttatcag atgccattct gttcgcaaaa aatgactaca
    12421 aattgctccc ggaaagccag caacagatcc aaaccatggc agccaaattg gcctcgacag
    12481 ggctaacaca tgcccgtatg gatggacaca ccgataacta tggtgaagac agttacaacg
    12541 aaggcttatc attgaaacgg gcgaatgtcg tggccgatgc atgggctatg ggtggacaaa
    12601 ttccacgcag caatctcacc acacagggtt taggaaaaaa atatcccata gccagtaaca
    12661 agaccgccca gggccgcgcc gagaaccgcc gcgtcgcagt ggtgattaca accccttaaa
    12721 acacgagcaa acagacaaaa aaagggccag ccaattggcc agcccttctt aacaggatgt
    12781 cgcttaagcg aaatcttagt taagacgctc tttgatacga gcagccttac cagtacgctc
    12841 acgcaggtag tacagtttag ctttacgaac agcaccacga cgtttgacag aaatgctgtc
    12901 aactaccgga gagtgagtct ggaagacacg ctcaacgcct tcgccgttgg aaattttacg
    12961 aacagtgaat gcagagtgca gaccgcggtt acgaatagcg ataaccacgc cctcgaatgc
    13021 ctgcagacgt tttttggaac cttcaacaac ccatactttc acttccacgg tatcacccgg
    13081 acggaaggaa ggtacgtcct gcttcatctg ctcttgttca agttgcttaa taatgttgct
    13141 cataatttaa tctcttatcc tgggtaaact gatatctcgg gggcttacgc catcccatca
    13201 tgtttatgtt gctgttgtgc gtgttccgtt ttgaactccg ccagcaacct tgcttgctct
    13261 tcagtcagag ccaggttttc cagaagttca ggtcttctaa gccaggtacg gcccagcgac
    13321 tgtttcaaac gccagcgacg tatctcggca tggttgcccg acagtaacac tggcggaact
    13381 tccatccctt ctaacacctc aggccgcgta tagtgcgggc aatccagcaa tccttcagca
    13441 aaggaatctt ccgttgccga ggcttcatgt cccagtactc ccggaataaa ccgggaaacg
    13501 gagtcaatca gcgtcattgc tggtaactca ccaccactga gaacgtaatc gccgattgac
    13561 cattcttcgt caatttcggt ttggatcacg cgctcatcta taccttcgta gcgaccgcac
    13621 accagaatca atttttgatt cgttgccagt tcgctgacgc ccgcttgatc aagcttgcgt
    13681 ccctgtggtg acagataaat cacctttgcg ccttcacccg ccgcggcttt tgctgcatga
    13741 atggcgtccc gcaagggttg caccatcatt aacatccccg gtccgccgcc gtaaggacga
    13801 tcgtccacgg tacggtgccg gtcatgcgtg aagtcgcgag gactccagct ctggatgctc
    13861 agcaggccat ttttaactgc ccggccagtt accccgtaat cggtaattgc gcggaacatt
    13921 tcaggaaaca ggctaattat gccaatccac atagcgccgt cttttaccgt ttatccggtg
    13981 gtttaaaaac caggatccca atctacttcg attgaacgag tagtgagatc gactttcttg
    14041 ataacctgcc catcgaggaa cggtacgaga cgttccttga taccaaacgc atctttcagg
    14101 tttgccttaa tgacgagaac gtcattagat ccggtttcca tcatatcgac gactttaccg
    14161 agatcgtagc cttcagtggt tactacctgg cagcccatca ggtctttcca gtagtagtcc
    14221 ccctcttcaa gctgaggcag ctgcgatgaa tccacgacaa tttcacaatt cgtcagcagg
    14281 ttcgccgcat cacgatcgtc aacgcctttc agcttgatga tcatgtcctg attgtggtgc
    14341 ttccagcttt ccagctggac ttgctgccac tgacccgcct tctggataaa ccaaggctga
    14401 tagtcaaaaa tgctttcggc gtcttcggtg gaagaaaaca ctctgagcca cccacgaata
    14461 ccgtaagacg aacccatttt tcccaaaacg atgggatcaa caggtgcttg cgcggtgagt
    14521 tgtttgctca tcatgaccac cgtgacagat taagctgctt tgtttacttc tttgatcagc
    14581 gcagcaacgc gatcagaaat agttgcgccc tggccaaccc agtgagcgat gcgatccaga
    14641 tccaggcgag tgccttcttc tttttcgcta gcgattgggt tgaagaaacc aacgcgctcg
    14701 atgaagcgac cgttgcgtgc attacggctg tcagcgacaa caacctggta gaacggacgc
    14761 tttttagcgc cgtgacgtgc taaacgaata gttaccataa catcctcttg tgtgaataaa
    14821 acaaccggac cccatcgagg aacggagtcc ggtgtcatat taaaagcccg aaaattttac
    14881 tcatttttgc gggaattgca atcaacagtt gctaactctg ctgtaaaagg ccgtcggcgg
    14941 tgcagccagt ttggtgccgg agtgcgcgca gtcaccggag cgtacacgca gtacgtgagg
    15001 atgacgagca catcccggtg ccaaaatggc aaacaagcca ggccgattag cgaccaggga
    15061 agcctggggg catcataccc ttcatgcttc tcatcatctt cgccattccg cctttcttca
    15121 ttttcttcat catgcgctgc atgtcgtcga actgcttcag aaggcggttc acgtcctgca
    15181 cctgcatacc gcaaccggca gcgatacgac gcttacgcga acctttgatg atttctggct
    15241 tagcgcgctc tttcatcgtc atcgagttga tgatggcttc catacgcacc agcactttat
    15301 cgtccatctg cgacttaaca ttatccggga tctgccccat gcccggcagc ttgcccatca
    15361 ggctagccat gccgcccata tttttcatct ggcgcagttg ctcaagaaaa tcgttgagat
    15421 cgaagccgtc gccttttttc agcttgctgg ctaatttctc tgcctgcgcg cggtcaactt
    15481 tgctttcgat atcttcgatc agcgacagta cgtcgcccat gccgagaata cgcgacgcga
    15541 tgcggtccgg atggaacggc tccagcgcct cagttttctc gccaacaccg agaaacttga
    15601 tcggtttgcc agtgatgtga cgaatagaga gcgccgcacc gccgcgggca tcgccgtcca
    15661 ctttggtcaa cactacgccg gtaagcggta acgcttcatt gaatgctttt gccgtattgg
    15721 ccgcatcctg accggtcatg gcgtcaacca caaacagggt ttcaaccggg ttaatcgacg
    15781 catggacttg tttgatctcg tccatcatcg cttcgtcaac gtgcagacga ccagcggtat
    15841 ccaccagcag cacgtcgtag aatttcagtt tggcttcttt cagcgccgcg ttaacgatat
    15901 ctaccggctt ctgaccaaca tcagaaggga agaaatcaac gcccacctgc tctgccagcg
    15961 tctcaagctg tttgattgcc gccgggcgat aaacgtcggc agaaaccacc agcactttct
    16021 tcttgtgctt ctcgcgcagg aacttaccga gtttaccaac gctggttgtt ttaccggcac
    16081 cttgcaggcc cgccatcagc acgaccgcag gcggttgcgc cgccaggttc agggtctggt
    16141 tctcttcgcc catcgccgca accagttcgt tacggacgat tttgacgaac tcctgccccg
    16201 gcgtcaggct cttattaact tcatgaccaa ccgctttctc ttttacgcga ttgataaact
    16261 cacgcactac cggcagagcg acgtccgcct ccagcagcgc catgcgcact tcgcgcagcg
    16321 tatcttttac gttgtcttca gtgaggcgtc cacggccact gatattgcgc agcgtgcgcg
    16381 acaaacgatc ggttaaatta tcaaacattg tctctcgcct ggggtggaaa cggttggccg
    16441 caatcgcgac acatcatcag tattttgccg cagtataaca tgaaggcgtc tttgttgtta
    16501 tgcaacggtt ggagcagcgt tcacctgacg ctatactgct tctctttctt attgctcaaa
    16561 ctgtcgacat cactatgccc gtttttgctc tgctcgcgct tgtcgcctac tccgtcagtc
    16621 ttgcgctgat tgttcccggt ctgctgcaaa aaaacggcgg ctggcggcgc atggctatta
    16681 tttctgcggt cattgcgctg gtctgccacg caatcgctct ggaagcccgc atcctgcccg
    16741 acggtgatag cggacaaaac ctcagcctgc tgaacgttgg ttcattggtc agtttgatga
    16801 tctgtacggt aatgaccatt gtggcttctc gcaatcgtgg ctggttgctg ctacccattg
    16861 tctatgcctt tgcgcttatc aacctggcgc tggcgacctt catgcccaat gaatacatca
    16921 cccatctgga agcaacgcct gggatgctgg tgcacattgg cttatcgctc ttttcctatg
    16981 ccacgctgat aattgccgcc ctgtacgcgc tacaactggc gtggattgat taccaactga
    17041 agaacaagaa gctggcgttt aaccaagaaa tgccgccatt gatgagtatc gagcgtaaaa
    17101 tgttccacat cacgcagatt ggcgtggtgc tactaacgct cacgctttgc actggcctgt
    17161 tctacatgca caacttattt agcatggaaa atatcgacaa ggccgtgctc tctatcgtgg
    17221 cgtggtttgt ctatattgtg ctgctgtggg gacattatca tgaaggatgg cgtggacgcc
    17281 gcgtcgtctg gtttaacgtt gcgggtgcag tcatcctgac actggcctac ttcggcagcc
    17341 gaattgtcca gcagttaatc agctaaaccc agaaaaggag tttcccctgg aacacatttc
    17401 tactactacg ttgatcatta ttctgatcat catggtggtc atttcagcct atttttccgg
    17461 gtccgaaacc ggaatgatga ccctcaaccg ctatcgtctg cgacatatgg cgaaacaggg
    17521 taatcgctcg gccaaacgcg tcgaaaaatt gctgcgtaag ccagaccgcc tgataagcct
    17581 ggtgttaatc ggcaataacc tggtcaatat tcttgcctcc gcgctcggca ctattgttgg
    17641 gatgcgtttg tacggcgatg cgggcgtggc aattgcgact ggtgtgctga cttttgtcgt
    17701 actggttttt gctgaggtat tgccgaaaac cattgccgcg ctgtacccgg aaaaagtcgc
    17761 ttatccgagt agttttctgc tggctccgct gcaaattttg atgatgccgc tggtctggtt
    17821 gctgaatgct atcacccgta tgctgatgcg catgatgggt atcaaaaccg atatcgtggt
    17881 tagcggctct ttgagcaaag aagagttgcg cactatcgtg cacgaatcac gctcacaaat
    17941 ttcccgtcgc aatcaggata tgctactgtc tgtgctcgat ctggaaaaga tgaccgttga
    18001 tgacatcatg gtgccgcgca gtgaaattat cggtattgat atcaacgatg actggaaatc
    18061 gattctgcgc caactctccc actcacctca cgggcgcatc gtgctctacc gtgattcgct
    18121 ggacgacgcc atcagtatgc tgcgagtacg tgaagcctgg cggttgatgt cggagaaaaa
    18181 agagttcacc aaagaaacca tgctgcgcgc cgcggacgag atctattttg tgccggaagg
    18241 tacgccgctc agcacgcagt tggtaaagtt tcagcgcaac aaaaagaaag tcggcctggt
    18301 cgtcaacgag tatggagaca ttcaggggct ggtgacggtt gaagatattc tggaagagat
    18361 tgtcggcgat ttcactacgt cgatgtcgcc aacacttgcc gaagaggtca cgccgcaaaa
    18421 cgacggttcg gtgattatcg atggcaccgc caacgtgcgg gaaatcaaca aagcctttaa
    18481 ctggcatcta ccggaagatg atgcccgcac ggttaatggc gtcattcttg aggcactgga
    18541 agagatccct gtcgcaggca cccgcgtgcg tattggcgag tacgatatag atattctcga
    18601 cgtacaggac aatatgatta agcaggtaaa agtttttcct gtgaaaccgc tgcgcgagag
    18661 tgtggcggag taacgaaaac ggccctgcat tcgcatgcag ggccgtgaat tattacgaaa
    18721 acagaaatta cgcttttgct ttcgctacag ttaccatcgc cgcacgaatc gtacgaccat
    18781 tcagcgtata acccttctgc ataatgccca gtacgttacc tggcgcaacg tcatcagatt
    18841 ccaccattgc gatagcctga tgcacattcg ggtccagcgg cacgttagtt tcggcgatca
    18901 cttcaacacc aaacttacgc acaacatcca gcatcgactt cagcgtcagc tcaatgcctt
    18961 caaccatcgc agacatatcc gggttagctt tatcagccac ttccagcgca cgatccaggc
    19021 tatcaatcac cggcagcaat tcgttgatga atttctccag cgcgaatttg tgggcttttt
    19081 caatatccag ttcagtacgt cgacgcaggt tttccatttc ggcttttaca cgcaaaatgc
    19141 cgtcacgttc acgggtctgg gcttcagcca gctgagcttc gagattcgca attttttcat
    19201 cgcgcggatc cacctgctca gcagaagctt ctggctcaac tgcctcaatc tcttcgtgct
    19261 gatccatgat aatttcttcc ggggcttgcc cctcaggcgt tttctgttct ttactactca
    19321 tgaatttctc cgcgtttttt tcgcattcat ctcgctaact tcgcttatta tggggatcag
    19381 tttcagggtt tcaagggaag cactcacatt gtcatcaatc ttcgcaacaa ggacctcgga
    19441 aaaatgaata atcatttcaa gtgtattggc attgtgggac acccacggca ccccactgca
    19501 ctgacaacac atgaaatgct ctaccgctgg ctgtgcacaa aaggttacga ggtcatcgtt
    19561 gagcaacaaa tcgctcacga actgcaactg aagaatgtga aaactggcac gctcgcggag
    19621 attgggcaac tagctgatct cgcggtagtc gttggtggcg acggtaatat gctgggcgcg
    19681 gcacgcacac tcgcccgtta cgatattaaa gttattggaa tcaaccgtgg caacctgggt
    19741 ttcctgactg accttgaccc cgataacgcc cagcaacagt tagccgatgt gctggaaggc
    19801 cactacatca gcgagaaacg ttttttgctg gaagcgcaag tctgtcagca agattgccag
    19861 aaacgcatca gcaccgcgat aaatgaagtg gtgcttcatc caggcaaagt ggcgcatatg
    19921 attgagttcg aagtgtatat cgacgagatc tttgcgtttt ctcagcgatc tgatggacta
    19981 attatttcga cgccaacagg ctccaccgcc tattccctct ctgcaggcgg tcctattctg
    20041 accccctctc tggatgcgat taccctggtg cccatgttcc cgcatacgtt gtcagcacga
    20101 ccactggtca taaacagcag cagcacgatc cgtctgcgtt tttcgcatcg ccgtaacgac
    20161 ctggaaatca gttgcgacag ccagatagca ctgccgattc aggaaggtga agatgtcctg
    20221 attcgtcgct gtgattacca tctgaatctg attcatccga aagattacag ttatttcaac
    20281 acattaagca ccaagctcgg ctggtcaaaa aaattattct aattttacgc cagcctcttt
    20341 actgtatata aaaccagttt atactgtaca caataacagt aatggttttt catacaggaa
    20401 aacgactatg ttggcacaac tgaccatcag caactttgct atcgttcgtg agcttgagat
    20461 tgattttcat agcggcatga ccgtaataac tggcgagacc ggcgcgggta aatctattgc
    20521 aatagatgcc ctcggtcttt gtctcggtgg tcgcgctgaa gccgacatgg tgcgtaccgg
    20581 cgctgctcgc gctgacctgt gcgcccgttt ttctctgaaa gatacgccag cggctctgcg
    20641 ctggctggaa gaaaatcagc ttgaagacgg gcatgaatgt ttgcttcgtc gagtgatcag
    20701 cagcgatggt cgctcccgtg gtttcatcaa cggtacagct gttcctctgt cacaactgcg
    20761 cgaactgggt cagttgctga ttcagatcca tggtcagcac gctcatcaat tactcaccaa
    20821 acctgagcac caaaaattcc tgcttgatgg ctatgccaat gaaacctctc tactgcagga
    20881 aatgaccgca cgttatcagt tgtggcatca aagctgccgt gacctcgcgc atcatcaaca
    20941 gttaagtcag gaacgcgccg cccgtgcgga actgctgcaa taccaattaa aagaacttaa
    21001 cgaatttaat ccgcagcccg gagagtttga acaaatcgac gaagagtaca aacgtctggc
    21061 gaacagcggt caattgctga ccaccagcca gaatgcattg gcattaatgg ccgacggtga
    21121 agacgcaaac ctgcaaagtc agctttacac ggctaaacaa ctggtgagcg aattgattgg
    21181 catggacagc aaactgtccg gcgtacttga tatgctggaa gaagctacca tccagattgc
    21241 tgaagccagc gatgaactgc gccactactg cgatcgtctg gatctagatc ccaaccgact
    21301 atttgaactt gaacagcgca tctcaaaaca gatttcgctg gcacgtaaac atcacgtcag
    21361 ccctgaggca ttgccacagt attaccagtc gctactggaa gaacagcagc aactggacga
    21421 tcaggccgac tcacaagaaa cgcttgcgct ggcggtaacg aaacatcatc agcaggcact
    21481 ggaaatcgcg cgcgcattac accaacaacg ccagcaatat gcagaagaac ttgcacagct
    21541 gatcaccgac agtatgcatg cgctctcaat gccgcatggg cagtttacga tcgatgttaa
    21601 atttgacgag catcacctgg gcgctgacgg tgccgatcgt attgagtttc gggtaaccac
    21661 caacccaggt cagccaatgc agcctattgc caaagtcgca tccggtggtg aattatcccg
    21721 catcgcactg gcaatccagg tcatcacggc gcgtaaaatg gaaaccccgg cactgatttt
    21781 tgatgaagtg gatgtaggga ttagcggtcc aacagcggca gttgtcggca aactgctgcg
    21841 tcaactcggc gaatcaactc aggtgatgtg tgttacccac ctgccacaag tcgcgggatg
    21901 tggtcatcaa cactattttg tcagcaaaga aaccgatggt gcgatgacag aaacgcatat
    21961 gcaatccctg aataaaaaag cgcggttaca agagctggcg cgcctgcttg gtggcagtga
    22021 agtcacacgt aatacactgg cgaatgcgaa agaactgctt gcagcgtaaa cttttttcct
    22081 gcttcacggt cagagtaaac agcaaaacgc cgtaagaccg gaaagcaaaa ggttttaaag
    22141 tgatgaaagg tctattatca tcggcatatt acagatgagc cacgtactgc tcgggcccga
    22201 aaaggaatca aatcactatg cgctgtaaaa cgctgactgc tgcagcagca gtactattga
    22261 tgttgaccgc aggctgttcc actctggagc gagtggttta ccgtcctgac atcaaccagg
    22321 ggaactatct gaccgctaac gacgtatcca aaatacgtgt tggcatgacg caacaacaag
    22381 ttgcgtacgc attgggtaca ccgctgatgt ccgatccatt tggtacgaat acctggttct
    22441 atgtcttccg ccagcaacca ggtcatgaag gtgtaacgca gcaaacgctg acgctgacct
    22501 ttaacagtag cggtgtgttg accaatattg ataacaaacc tgcgctgagt ggtaactaat
    22561 aataaagttg ctctcaaaga cgttaaaaaa ggtgctcaat gagcaccttt tttctgtctg
    22621 ttatttattc gctgattttt ctgctcgttg cctgcgaagc tctttcggat cggcaatgag
    22681 agggcgataa atctccaccc gatcgccatc atgcacacta tcgcttagtt ttgccggacg
    22741 gctgtaaatg ccgactttgt ttttagttaa atcgatatcg gtacgcaatt ccagcaagcc
    22801 actggcgcga atagcttctt caaccgtcgc gccctcctgc agcgtcacgc gctgcaggta
    22861 ctgcttctca ggtagcgcat aagccacctc aacggcaatt ttacctggca ctgtaaacct
    22921 ctttcgcacg aaccgtaaaa gcctggacca tattagacgc cagctcttta aacacgcgac
    22981 caaaggcgag ttcaatcaac ttattggtaa actcaaagtc gagatgaaac tcgatacgac
    23041 acgcctcctg gctcagcggc gtaaacttcc atccaccaat caatttcttg aacggcccat
    23101 ccaccagatt cataagaata ctttggttac tggtcaactg gttgcgggta gtaaacgttt
    23161 tgctgatccc agccttagag acatctaccg ccgcagtcat ctgcccagga gtggactcca
    23221 gaatccgact tccggtacaa cccggcaaaa actgaggata agactgaacg tcattcacta
    23281 actgatacat ttgctccgcg ctgtagggta ccagtgcggt ccggctaatc tgaggcataa
    23341 caatttccat caacaaaaat ccaacaaata atatcattta tcctgctgta aaaaaaacgc
    23401 tatcccggcg ctgggtaaca tcgggttcat gctaagatag agccttgtcc cccgcaggat
    23461 tgatatgggg tgttttcgat ttcagattac cgatgattca cgacgcttat gacgaagaaa
    23521 aaagcacata aacctggttc agcgaccatc gcgcttaaca agcgcgcccg tcacgaatac
    23581 tttatcgaag aagagttcga agcgggactt gccctgcaag gctgggaggt taaatccctg
    23641 cgcgcaggaa aagccaatat cagcgacagc tacgtccttc tgcgtgacgg agaggcattt
    23701 ctgtttggcg ctaacatcac gccaatggcc gtggcctcca cgcatgtggt gtgcgatcct
    23761 acccgtaccc gcaagttact tctcaaccag cgcgaactgg actcattgta cggtcgcgtc
    23821 aatcgagaag gctataccgt agtggcgctc tccctgtact ggaaaaatgc ctggtgcaaa
    23881 gtgaaaatcg gcgtcgccaa aggtaaaaaa cagcacgata aacgttcaga tatcaaagaa
    23941 cgcgaatggc aggtggataa agcacgtatc atgaaaaacg cccaccgtta aacctgcact
    24001 ccaattattg accagttcct caccgcgcct ccctctccgg cggcgcgaat gaacatctta
    24061 ttggctatca catccgacac aaatgttgcc atcccattgc ttaatcgaat aaaaatcagg
    24121 ctacatgggt gctaaatctt taacgataac gccattgagg ctggtcatgg cgctcataaa
    24181 tctggtatac ttacctttac acattggggc tgattctgga ttcgacggga tttgcgaaac
    24241 ccaaggtgca tgccgagggg cggttggcct cgtaaaaagc cgcaaaaaat agtcgcaaac
    24301 gacgaaaact acgctttagc agcttaataa cctgcttaga gccctctctc cctagcctcc
    24361 gctcttagga cggggatcaa gagaggtcaa acccaaaaga gatcgcgtgg aagccctgcc
    24421 tggggttgaa gcgttaaaac ttaatcaggc tagtttgtta gtggcgtgtc cgtccgcagc
    24481 tggcaagcga atgtaaagac tgactaagca tgtagtaccg aggatgtagg aatttcggac
    24541 gcgggttcaa ctcccgccag ctccaccaat catgattgga cggtataagg acaacaccaa
    24601 taaaaacagg aagttagcag tctcagcagg acaccgacca gacggtgaag agacataaaa
    24661 ggatacgcaa aggagccgcg gctcctggtg acatgaaagc ccacagatgt gggctttttc
    24721 gttgatggtc agaacgacca gttcacacca gctaccccgt tccacgggga ttccacaccg
    24781 gcaccatggc tataccccac cccaagatgc ccgcttaacg tactgctgaa tgaggcttta
    24841 atacctgcct ggtatattcc acgtctgccc gacaaatcat tgacgaaatt accgtcacta
    24901 ttcactttca cccggttatc atcgataaat tctttgcgca cagccgcctt cagccacggc
    24961 tcaacttcca taccgttccc cagacgcatg ttgtaactca gcgttgcacc cagttcacga
    25021 tataaactgc gggtatcgac tgatttcgat tccatgccat tggataaatg atattcaggg
    25081 ttatcagcgg tgaacccccg ttaacgatgc atacggcgtc aggttccagt taccatcggt
    25141 aaatcgcatc ccggtttcaa tgtgaccgcc cagcccgttg ctgcgataac tgccgttggc
    25201 ggctccaccg ctgccaaccg tggagtcgcg agcttcggta cctgctaata ccaataaatt
    25261 accgccattt tccagcaaca tattggtcgc taaattgccg gaaatggaaa aagtgccgta
    25321 ctggtgagta ccgctgattt caataccgtt agccgtgctc gtctggagag cggcaccgct
    25381 gttctggacg atatctgtcg ctttgccatt atcgttaact gtcagcgtac cgccttcatt
    25441 gatctttgtt tttattgcct ctccgttagc tgaaactgtt tgtattccgc cgtcgttaat
    25501 cgttgtctca ttcgccaccc cctcgacgat ttgttcgccg ccggtgagcg tcgtgcctgt
    25561 cgcagtggct tttgttttga cgatctcccg tccgcccgta ttgacctgtg ttttgtcaga
    25621 agaggtgtct gactccatgg ttaacacgcc gccatttgcc agcaggatat tgttcgccgc
    25681 accctgctcg atgctgaacg cgacgccatc cgcgcgtgtt cctgtgaccc gcgtcgccct
    25741 ggtggttgca accaaagcgt cctggctact ctgctgtatc cccgttgcgc tgcctttctc
    25801 ccgcacatcg agtatgccgc cgtcattaag caccgagttt tcagccagac cgccctcatt
    25861 aactacctgt gaacacccat taataatgga accttccgct gtcccgtttg ccataatttg
    25921 ttgtaggcca gagacgatat cggtattgat tgccttacca taattctgaa cggtttgcgt
    25981 gccaccattg atgtgtg
//
LOCUS       DAZGVQ010000065        25854 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000065 DAZGVQ010000000
VERSION     DAZGVQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.065"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..265)
                     /locus_tag="ACHLAG_002554"
     CDS             complement(<1..265)
                     /locus_tag="ACHLAG_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP0655760.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIE"
     gene            478..1269
                     /locus_tag="ACHLAG_002555"
     CDS             478..1269
                     /locus_tag="ACHLAG_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFP0655761.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            1279..2121
                     /locus_tag="ACHLAG_002556"
     CDS             1279..2121
                     /locus_tag="ACHLAG_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP0655762.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIAHQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            2131..2907
                     /locus_tag="ACHLAG_002557"
     CDS             2131..2907
                     /locus_tag="ACHLAG_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HFP0655763.1"
                     /translation="MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNIPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            2917..4458
                     /locus_tag="ACHLAG_002558"
     CDS             2917..4458
                     /locus_tag="ACHLAG_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFP0655764.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHIMAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            4455..6536
                     /locus_tag="ACHLAG_002559"
     CDS             4455..6536
                     /locus_tag="ACHLAG_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFP0655765.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            6557..7033
                     /locus_tag="ACHLAG_002560"
     CDS             6557..7033
                     /locus_tag="ACHLAG_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFP0655766.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            7076..8377
                     /locus_tag="ACHLAG_002561"
     CDS             7076..8377
                     /locus_tag="ACHLAG_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFP0655767.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIVLIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFL
                     ESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            8430..9401
                     /locus_tag="ACHLAG_002562"
     CDS             8430..9401
                     /locus_tag="ACHLAG_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFP0655768.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQEVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(9515..9964)
                     /gene="rplI"
                     /locus_tag="ACHLAG_002563"
     CDS             complement(9515..9964)
                     /gene="rplI"
                     /locus_tag="ACHLAG_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFP0655769.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(10006..10233)
                     /gene="rpsR"
                     /locus_tag="ACHLAG_002564"
     CDS             complement(10006..10233)
                     /gene="rpsR"
                     /locus_tag="ACHLAG_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFP0655770.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(10238..10552)
                     /gene="priB"
                     /locus_tag="ACHLAG_002565"
     CDS             complement(10238..10552)
                     /gene="priB"
                     /locus_tag="ACHLAG_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFP0655771.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(10559..10954)
                     /gene="rpsF"
                     /locus_tag="ACHLAG_002566"
     CDS             complement(10559..10954)
                     /gene="rpsF"
                     /locus_tag="ACHLAG_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFP0655772.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            11281..11556
                     /gene="yjfY"
                     /locus_tag="ACHLAG_002567"
     CDS             11281..11556
                     /gene="yjfY"
                     /locus_tag="ACHLAG_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFP0655773.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(11685..12371)
                     /gene="ulaF"
                     /locus_tag="ACHLAG_002568"
     CDS             complement(11685..12371)
                     /gene="ulaF"
                     /locus_tag="ACHLAG_002568"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFP0655774.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIASFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12371..13225)
                     /gene="ulaE"
                     /locus_tag="ACHLAG_002569"
     CDS             complement(12371..13225)
                     /gene="ulaE"
                     /locus_tag="ACHLAG_002569"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFP0655775.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGREIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(13235..13885)
                     /gene="ulaD"
                     /locus_tag="ACHLAG_002570"
     CDS             complement(13235..13885)
                     /gene="ulaD"
                     /locus_tag="ACHLAG_002570"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP0655776.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(13899..14363)
                     /gene="ulaC"
                     /locus_tag="ACHLAG_002571"
     CDS             complement(13899..14363)
                     /gene="ulaC"
                     /locus_tag="ACHLAG_002571"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFP0655777.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(14373..14678)
                     /gene="ulaB"
                     /locus_tag="ACHLAG_002572"
     CDS             complement(14373..14678)
                     /gene="ulaB"
                     /locus_tag="ACHLAG_002572"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFP0655778.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(14694..16091)
                     /gene="ulaA"
                     /locus_tag="ACHLAG_002573"
     CDS             complement(14694..16091)
                     /gene="ulaA"
                     /locus_tag="ACHLAG_002573"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP0655779.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            16446..17510
                     /gene="ulaG"
                     /locus_tag="ACHLAG_002574"
     CDS             16446..17510
                     /gene="ulaG"
                     /locus_tag="ACHLAG_002574"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFP0655780.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            17618..18373
                     /gene="ulaR"
                     /locus_tag="ACHLAG_002575"
     CDS             17618..18373
                     /gene="ulaR"
                     /locus_tag="ACHLAG_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFP0655781.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(18370..19119)
                     /gene="yjfP"
                     /locus_tag="ACHLAG_002576"
     CDS             complement(18370..19119)
                     /gene="yjfP"
                     /locus_tag="ACHLAG_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP0655782.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVRCTASMMGSGYFTSLARSLFPPLIPETTA
                     QQNEFNNIVAPLAEWEATNHLEQLSDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            19301..19630
                     /gene="bsmA"
                     /locus_tag="ACHLAG_002577"
     CDS             19301..19630
                     /gene="bsmA"
                     /locus_tag="ACHLAG_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFP0655783.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            19779..20054
                     /gene="yjfN"
                     /locus_tag="ACHLAG_002578"
     CDS             19779..20054
                     /gene="yjfN"
                     /locus_tag="ACHLAG_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFP0655784.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(20171..21796)
                     /locus_tag="ACHLAG_002579"
     CDS             complement(20171..21796)
                     /locus_tag="ACHLAG_002579"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFP0655785.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELRREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(21880..23043)
                     /locus_tag="ACHLAG_002580"
     CDS             complement(21880..23043)
                     /locus_tag="ACHLAG_002580"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP0655786.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRATVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(23046..23684)
                     /locus_tag="ACHLAG_002581"
     CDS             complement(23046..23684)
                     /locus_tag="ACHLAG_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFP0655787.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(23694..24092)
                     /locus_tag="ACHLAG_002582"
     CDS             complement(23694..24092)
                     /locus_tag="ACHLAG_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFP0655788.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(24110..24769)
                     /locus_tag="ACHLAG_002583"
     CDS             complement(24110..24769)
                     /locus_tag="ACHLAG_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFP0655789.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(24820..25518)
                     /locus_tag="ACHLAG_002584"
     CDS             complement(24820..25518)
                     /locus_tag="ACHLAG_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFP0655790.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(25537..>25854)
                     /locus_tag="ACHLAG_002585"
     CDS             complement(25537..>25854)
                     /locus_tag="ACHLAG_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFP0655791.1"
                     /translation="LIIKMNDYGDLQINILFTSRQMIIETFICPVSSISNPDEFNTFL
                     LRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLEITSLVDNALDLAEITEE
                     YSH"
BASE COUNT         6461 a         6651 c         6376 g         6366 t
ORIGIN      
        1 tctcaatagc tttttgtggc ggaagatctg agaaatcaag ggccttttct ttttgtcgaa
       61 tatcgttcca ggtatattcc agcacttcga taaataaatt ttctttatta ccaaagtggt
      121 gataaaccgt ctgtttacta caccctgctg cctttacaat attatctact ctggcccctt
      181 cataaccata ttcggcaaat tcattaaccg cacatagcag caatttttcc ttcaggctct
      241 gtacgttgct ggtatcaata tacataacgc ttccccacga cctcttcaag ataactatct
      301 taacccgatt aattagaagc tccagtaaaa aggataaaaa cttgtacttt catcaagtta
      361 tcaaatgtac tgtgatcact atcgcatatc aaccaaccag atggttgatc tttcttgcgg
      421 cacgtcgcaa gctaattgtc acttagaaaa caatattcaa aaaaagagag ctattgtatg
      481 aaatccactc gtcctgttgc cgtgattacc ggagccgctc gtggtattgg aaagggctgt
      541 gcgctggagc ttgctcgtgg cggatttaac ctgctgatta acgatcgtcc cgatgccgac
      601 agcgtagaaa aactgcatat cacacaacaa gaatgtttgg cagaaggcgt ggaagtcatt
      661 tgcttccctg ccgatgtcgg cgatctctcc ttgcatgaag agatgctcga cgcggcgcaa
      721 aaccagtggg ggcggctgga ctgtctgctc aataacgcgg ggatttcagt gaaaaaacgc
      781 ggtgacctcc tcgacctcga accagacagc ttcgatcaaa acattgccat taacacccgc
      841 gcgccgttct tccttgctca ggcattcagt aagcgcttac tggcacagcc gaaaccggaa
      901 gcggagctgc cgcaccgctc gattattttc gtcagttcca tcaacgccat catgctggcg
      961 atgaatcgcg gcgaatacac cattgccaaa accgccgttt ctgccgctgc tcgtctgttt
     1021 gctgctcgcc tttgtaatga acaaattggc gtttatgaag tgcgccctgg tctcatcaaa
     1081 accgatatga ctattccggc aacggcttat tacgacgaac tgattgccaa aggtctggtg
     1141 ccgtggggac gttggggtta tccggctgat attgcttcca ctgtccgcgc gatggcggaa
     1201 ggtaaactga tttacacctg tggtcaggcg gtcgccatcg acggcggctt aagtatgccg
     1261 cgcttctgag gctgaatcat gagagatctg ttacaacgcc cggatttatt cagtatcaat
     1321 accgcaacac tgggctataa aacgccgcta cccgccatta ttgatgcctg cgccgcgcgt
     1381 ggtattggcg cgattgctcc ctggcgcagg gagttgcaag gtgaagatct ccagcaaatc
     1441 gcccaccagc ttgccgccag taacatgagc gtttccggtt tgtgccgtag cacttactac
     1501 accgcgccga cactggcgga acgcaaactg gcgattgatg ataaccgccg ggcgctggac
     1561 gacgcagcgg tgcttaacgc cgcctgttat atgcaggtgg ttggcggtct gccaacgggc
     1621 acaaaagatc tgtacgaagc gcgggaacaa gtaaaacaag ggattcgcca gttgctgcca
     1681 cattcgaaag atgtcggcgt gccaattgcg ctggagccac tgcacccgat gacagctgcc
     1741 gatcgctctt gcctgtgcac gttacgccag gcgctggact ggtgtgatga actcgatccc
     1801 gatggtgagt ttggcctcgg cgtggcggtg gatgtttatc acgtctggtg ggatccggat
     1861 ctcgccagtc agatcctgcg tgccggaaaa cgcatcctcg cgtttcatgt ttccgactgg
     1921 ttagtgccga ccactgatct ggtcaatgac cgtggtatgc cgggagatgg cgtcatcaat
     1981 attccgtcaa ttcgtcggct ggttgaaaac gccggattta atggtgctat tgaactggaa
     2041 atattctcgc cttactggtg gcagaaagat attaacagca cgctggatat tagcgtcgat
     2101 cgcatcgcgc attattgtta aggaatgatc atgagtgacc aacccgttct gttcagcaga
     2161 gtggcggctt cctgccgcct gacattgaac cgtgaagata aatgtcatgc tattaacgaa
     2221 gagatgatcg aatctctcga ccattattta aatgaaattg aaaacgacac cacattacga
     2281 ttagtcgaat taacggcaac cggcgataaa ttcttttgtg cgggcggcga tattaaatcg
     2341 tggtcggcct attcgccatt agatatgggc agaaaatgga ttaaacgcgg taatgacgtt
     2401 tttaatcgcc tgcgaaacat accccaatta accgttgcga atctcaacgg tcataccatc
     2461 ggcggtggta ttgaactggc cttgtgctgc gatattcgta ttgcccgccc cggcgcgaaa
     2521 ttctctaacc cggaagtgat gctcggcatg gtgcctggct ggatgggcat tgaacgtgtg
     2581 cttaatcagg ttggtccggt ggtcggtcgc cagatgctga tgctgggtaa acgcctgacg
     2641 gcgcaggaag cccaggcggc gaatttaatt gatgaagtgg tagaaaaaga acaggtggaa
     2701 agctggatgg cgaaccagtt agcgcagctg gaaaaatgcg gtccggtggc actggcgcat
     2761 attaagcaac tgattctggc actggaaaat aaacatgccg attacccgca ccagctactg
     2821 gccggattaa tgtctgccac tcaggattgc cagcaggcga cgcgcgcctt tgccgaaaaa
     2881 agtagcgtga gcttccataa tcaataagga aattgtatgc gtaagataac aaccgccgag
     2941 gcactggcgg cgcaaattca ggacggcgca accattgcca ttagcggtaa cggcggcggc
     3001 atggtggaag ctgaccatat tatggccgcc attgaagcgc gtttcctgca aaccggacac
     3061 ccgcgcgacc tgacgctaat ccactcgctg ggtattggcg atcgcgactg caaaggcact
     3121 aaccgttttg cgcacgcaga gatgctcaaa cgcattattg ccgggcactt tacctggtcg
     3181 cccaaaatgc aggcgctggt aaaaaataac acgattgaag cctactgctt tcctggtggc
     3241 gtgattcagg cactgctacg ggagatcggc gccggacgtc cggggctttt tacccacgtt
     3301 gggctgggat cgtttgttga tccacgcaat ggcggcggta agtcgaatga atgcactacc
     3361 gacgacctgg tagaactgat tgaaatcgac ggtgaaacca aacttcgtta tcgccctttc
     3421 aaagtggatt acgcgatttt gcgtggcact tatgccgatc ctcgaggcaa cgtcagcctt
     3481 gaagaagaag cgattgatat ggatagctat tccatggcgc tggcagcaca caacagcggc
     3541 ggcaaagtgt tcgtacaggt acgcgatgtg ctggaagctg gcgcgattga accacgccgg
     3601 gtcaaattac cgggcattct ggttgatggc atcgttgaac accgcgaaca accacaaacc
     3661 tatcttggcg gttacgacct gaccatcagc ggccaacatc gccgtctgag ttctaacgac
     3721 gccattgaac tggttagtca tccggtacgt cgcctgattg cccgtcgggc ggcacgcgaa
     3781 ctggtagctg gcgcttcaac caactttggc tttggtattc ctgggggtat tccaggcgta
     3841 gcgctgcgcg aaggcgtgcc ttaccaaagt ttatggctga gcgtcgagca aggcgtgcat
     3901 aacggtatga tgctggatga tgcgtttttc ggctgcgccc gcaacgccga tgccattatt
     3961 ccatcactcg atcaatttga gttctacagt ggcggaggga tcgatatcac cttcctcggc
     4021 atgggagaga tggatcagta cggtaacgtc aacgtctccc atctcaatgg caatctgatt
     4081 ggccccggcg gatttctcga aattgcgcaa aacgcccgta aagtggtgtt ctgcggcacg
     4141 ttcgacgcca aaggtagcaa gattgatgta acgccagatg gcttgcatat cacccagtca
     4201 ggtcaaatcc ctaaactggt tacccaggtg gaaaaaatca cttttagcgc cgcctacgca
     4261 cagcaaagtg gtcaggaagt gttgtatatc actgaacgcg cagtattcca gttaacggca
     4321 gaaggcgttg aattaattga aatcgcgccg ggtgtggaga ttgagcgcga cattctgccg
     4381 tatatggctt tccgtccaat tatcaagcac ccacgcctga tggaaagtag cctgtttacg
     4441 ccgatggagg atgcatgagt cagcttgatg acaccatcct tgatgcgctg acgcacgtta
     4501 ctttcccaaa gggttttgca caggcagagc ccgcatgggt tgtcacggta gacggtgttg
     4561 attacccact ctggcaaaca gatgccctgg tagtcggcag tggtgcagca ggattacgtg
     4621 cggcagttga acttaaacgc cgccagcaaa atgtgctgat cgctaccgcc gggttatata
     4681 tggggacgtc ggcatgttct ggttccgata aacagacact atttaccgcc gctaccgcgg
     4741 gcaacggcga caacttcacc aaactggcag aggcactggc gagcggtggg gcgatggatc
     4801 acgacaccgc ttatgtcgaa gcggtaggtt ctctgcacac tctaggcggg ttgcaatatc
     4861 ttggtctgga attaccggaa gatcgctatg gcgcgattct tcgttatcaa accgaccatg
     4921 acgaagccgg gcgtgcaacc tcgtgtgggc cgcgtacctc aaggttaatg gtgaaagtgc
     4981 tgttggaaga agtacagcgc ctcgccattc cagtgttgac cagtgcaaca gtgattaaac
     5041 tgctgcatca gcgtgacgaa aacggcgaag accgcgtggc aggggcaatc ctcgcgaccg
     5101 gtcatcgcgc ccataaccca tgggggctgg caattgtgac tgcgcccaat gtggtactgg
     5161 caacaggagg gcctggcgag ctttatcgcg acagtgttta tccacataaa tgcttcggct
     5221 cgctggggct ggcgctggag gaaggtctga cgctaaccaa tctgaccgaa agccagtttg
     5281 gtataggcac gccgcgtagc acgtttccgt ggaatttatc cggcacctat gtacaggtga
     5341 tcccgtatat ctattccgtg gatgctgagg gtaacgagta taacttcctc gcggattact
     5401 atcgcaccac ccaggagctg gcttcaaaca ttttccgtaa aggctatcag tggccgttcc
     5461 acgccacgcg ggtgatggat tttggctcca gcttgttaga tatggcagta gcgcaagagc
     5521 agcaatcagg gcgtcaggta tttatggatt tcaatcgcaa tcctgaagcg gtgccgggtg
     5581 acctgccgtt ctcattagat cgactggacg acgacgttcg cgcgtatctg gaaaataacg
     5641 acgctctggc accatcgccc atcgaacggc tgcaacgaat gaatccgctg tctatctcgc
     5701 tgtataagat gcacggttac gatctcacca cacagccttt gcagtttgcc atgaataatc
     5761 agcatatgaa tggcggcatt gaagtggata tctggggaca aacctccctg cccggctgtt
     5821 tcgccgtggg ggaagttgct ggcacacacg gcgtcactcg ccctggtggt gcagcattga
     5881 atgccgggca ggtttttgct gttcgtctgg cacgttttat tggttgcacg caaaagcgga
     5941 atattgatgg agatatagca cagctggtag ctcctacact ggcttctata agagagatca
     6001 ttactcaggc gcacgataac gggaccggga tgccgttgtc ggttgtgaga gaaaaaattc
     6061 aggcacgaat gtctgaccat gcgggattta tttgccatgc cgataaagtc cgacgcgcca
     6121 ctcgcgatgc cctgctattg agcgaatttg tccaacggca tggattggct atcaaacatg
     6181 tgggtgaagt tgccgagctg tttatgtggc ggcatatggc actgacttct gccgccgtat
     6241 taacgcaatt aacgcattat attgacgcag gtggtggtag tcggggagca cgtattattc
     6301 tcgacaggga tggaaattct attccgcaaa cacgaaatgg tttttgtgat gcctggcgct
     6361 ttcgttcaga gcgtactgag gataaaaaag ataaactact tattcattat tgcaacggta
     6421 tctttcacgt cagagaaaca cctgttcgtg agtttcctat tatccgaggt atttggtttg
     6481 aaaaaaactg gcctggtttt cttaatggga caatttacca gccacaggat gaatgatatt
     6541 gcaaaggtta atcactatgc gaaatataat tagatctatt aatgctctgc ttgcagcatt
     6601 gaacattacc attttggcga tcatcgtggc atgtgtgacg tggcaggtgg ctgcacgatt
     6661 tatatttact agccccagta tttttacaga tgaactcagc cgtttgctct ttatttgtct
     6721 tggacttttt ggtggggcat acaccgcagg gcaaaaccgg catctggcga ttgacctgct
     6781 acctatgatg ctgaaaggaa aagcccgacg tcatttattt ttgtgtattc agattatcgt
     6841 tattatcttc gcaaccatca ttatggtata tggtggtggg ctattaacga tggatacatt
     6901 cgattcaggt cagacctcgc ccgctttagg ctggcaaatg ggatatatct atatgagcat
     6961 cccgattagt ggtgtactta ttattatata caccatagat atggttttaa ccgaacttaa
     7021 acaaccactc taaagataac aaacaacaac caaccatagt aggaatggag aaattatgga
     7081 ttactggtta ccgattattg tgttatttgg tgccttcttt tttatgttgg cattaggtgt
     7141 acctattgtt tatgccattg gtttatcgac actggcatca atttccacac aactcgattt
     7201 caactcagca ttgagtgtgg tttcccaaaa gttagcctcc gggttagata gttttacgtt
     7261 gttggcaatc ccatttttta tcttgtctgg caacattatg aatcatggag gaatagcgcg
     7321 acgattaatc aactttgccc gtattctcgg cggtcgtctg ccaggctcac ttgcccactg
     7381 taatattctc gccaatatgc tgttcggggc aatttctggt tcagccgtgg cttcggcggc
     7441 tgcgatgggc ggcgtcatgc atccgcaaca ggttaaagag ggctacgacc cggcattcag
     7501 tactgcggtt aacgttgcct ctgccccgac gggtttgctt attccaccaa gtaatacgtt
     7561 aatcgtctat tcactggtca gtggtggtac ttctattgcc gcactgttcc tggcgggtta
     7621 tgttccgggg attttgctgg gtctggcact gatggtaata gccggtatta tcgctgtacg
     7681 tcgcggttat ccaaaaccag aacgtccgac tttgcgccag gctggcgttg cgatatggat
     7741 ggctatcccc agtattttcc tgattgtgtt aattatgggt ggcgtactaa gcggtatttt
     7801 tactccaacc gaagcctccg caattgctgt aatttatacc cttttccttg cactggttct
     7861 ttatcgtgaa atctcagtta aagatttacc taaaatattt ctggaatccg ttattaccac
     7921 tgcaattgtc ttgttattaa ttggttcttc gatgggaatg tcatgggcaa tgtcaaacgc
     7981 tgacgttccc ttcctgattc tcgatttgct taacactatt tctgacaacc caataataat
     8041 cctgcttatt atcaatatta tcctgttgat tatcggcact tttatggata tgacaccggc
     8101 ggtactgatt tttacgccga tatttttacc ggttgtcacc gaactgggca tggacccgat
     8161 tcactttggt attgtcatgg tgctgaacat gtgtatcggt atttgtactc cgcctgtagg
     8221 cagtgtgtta tttgtcggtt gtagtgtatc taaattaccg attaataaaa ttatcaaacc
     8281 tatgctgcca ttctatgccg tgatggtatt ggtgctggca atggtgacct atattccgca
     8341 aatcagtatg gcattgccaa gagcactggg ttactgatgc gcattcacta gcggcacctg
     8401 ccgccatatt tattagaaaa ggaacatcta tgaaaataaa agtatccgct ggcattatcg
     8461 gtgctgttct tatgttatcc gcaagccagt cctgggcagt gacattaaaa ctgagtcata
     8521 atcaggataa gtctcatcct gttcataaag cgatggagtt ctttgcgaaa aagagcaaag
     8581 agtactctaa cggtgatatt actattcgta tttatccaaa tggaacattg ggtactcaac
     8641 gagaaacaat ggagctgatt cgttctggcg ctattccact ggtaaaaacc aatgcggcag
     8701 aaatggaagc atttgaaaat tcctataaat tatttagcct gccttatttg ttccgcgatc
     8761 gtgatcatta ttatcaggtc atgcagggcg atatcgggag aaaaatcctc gactcaacga
     8821 aaagcaaagg ttatttcggg ctgacttttt atgatggagg cgcccgcagt ttctatggca
     8881 ataaaccagt actgaaacca gacgatctga aaggcatgaa agtccgtgtc cagccaagcc
     8941 ctggcgcagt tgaaatgatc aaagtcatgg gcggtaaccc gacgccactg gattacggcg
     9001 agttgtatac agccttacag cagggtgtgg tcgatatggc agaaaacagc gtgatggcgc
     9061 tgaccaccat gcgtcacggt gaagtggcaa aatccttcag ccttgacgaa cacactatgg
     9121 ttcccgatgt ggttctgatg agcaatgctg cgtttgataa acttagcccg gaaaatcagg
     9181 aagttatatt aaaagcagct aaagaatcaa tgagctacat gaaagacttg tggagcgagg
     9241 aagagaaaca agaatttgca aaactggata aaatgggcgt gaaagtctac caggtagata
     9301 aagctccgtt tatcgagaaa gtacagccaa tgtacgcaaa cttcgctaag gataacccag
     9361 cccttgcccc aatgctggct gatattcagg cggctaagta attatcttcg tagggctgct
     9421 tattgtggcc cttatcgata tctgcaagtg ctcgctacaa aagcaaaacg ccgaccaatg
     9481 gtcggcgttt ttttacgtct cgttgaataa cgaattattc agctactacg tttacgatca
     9541 ctttcgcgaa tacttcgctg tgaacctgga agctcacttc gtgttcgcca gtggtacgca
     9601 gaacgccgtt cggcagacga acttcgctct tagccacttc aacgccagct gcagttacag
     9661 cgtcagcgat gtcgcgagta ccgatggaac cgaacagttt accttcgtcg ccagctttag
     9721 acgcgatggt aacagtttcc agtgcattga ttttctcagc gcgagcatta gcagctgcca
     9781 gaacttcagc cagtttagct tccagttcag cgcgacgtgc ttcgaagaat tcaatgtttt
     9841 tcttggtagc tggaacagct ttaccctgcg gtaccaggaa gttacgagca tagcccgctt
     9901 taacgtttac ctgatcaccc aggctaccca ggtttgctac tttatcaagc agaataactt
     9961 gcattacctt atcctctcaa agtcgtatta atggaccgtg accgattact gatggcgatc
    10021 agtgtacggc agcagggaca ggtagcgagc gcgtttgata gcgcgagcca gctgacgctg
    10081 gtattttgca cgggtaccgg tgatacggct tgggacaatc ttaccgcttt cggtgatgta
    10141 gtttttcagc gtagcgatat ctttatagtc gatctcttga acgccttccg cggtgaaacg
    10201 gcagaacttg cgacgacgga aataacgtgc catatggcta gtctccagaa tctatcaatt
    10261 caatctgctc ggcatgcaaa accattttgc tcagtccgtt ctttgccttg tggcatgaaa
    10321 tgaacccctg aacggttatg cgactgccga ccgttatact gtgagtaatg gcctggtttt
    10381 cgtgtccgct aacaataacg ggcatttgac accatgcctg ccggtgaaag ccggcttcct
    10441 cctgcacaga acgatgctca agcacgaact ggcagtgagg aattcctgat ggactgacct
    10501 ttcgaagggg agtcctgcac acggtgccgg acaacaccag acggttggtc atcagaaatt
    10561 actcttcaga atccccagct tcagcatcat cagcggtttc gtttgcgaaa tcatcgcgac
    10621 gctcacggcg ctcgtctttc gctttaacca tcggagatgc ttcggtaaca gcgtgcttgg
    10681 tacgcataac catgctgcgg ataacggcat cgttgaagcg gaaggtagtt tccagctcat
    10741 cgatcacttc ctgcggagct tcaacgttca tcagaacgta gtgtgctttg tgcagtttgt
    10801 tgatcgggta agccagctga cggcggcccc agtcttccag acggtggatc ttgccttctg
    10861 caccagtgat ggcagcagtg tagcgctcga tcatgcccgg aacctgttcg ctctggtcag
    10921 gatggaccat aaaaacgatt tcgtaatgac gcatcgaatt gctccttacg gattattcag
    10981 cctcctgtct gggtcagccg aatcccgggg aggcaaggaa cgtgttaaag gtcggctgaa
    11041 aaatgacgcg ttatagtact tgtatgcccc gctaaactca aggccagccg acaaataaat
    11101 ctcatctttc gctcaaaaga actaactccc tgatttgaag ttcatcacat tccgtcacag
    11161 tgttttttcg aacaactgca tcagaaatgg tcaccatcaa cttctttatc attgattaaa
    11221 attaaatcag aagatcatca cagaggagag cacacaaagt cagccgtcga ggaggtatct
    11281 atgttcagtc gtgttttagc ccttctggct gtgcttttgc taagtgcaaa tacatgggca
    11341 gccattgaaa tcaataacca ccaggccaga aatatggacg atgtgcaaag cttaggcgtg
    11401 atttatatca atcataattt cgccactgaa agtgaagcac gtcaggcatt aaatgaagaa
    11461 acagatgcgc agggcgcaac gtactaccac gtaattctga tgcgggaacc ggggagtaac
    11521 ggcaatatgc acgccagcgc ggatatttat cgctagcacc aggtataacc aacgaaacat
    11581 tgccatagtt tgctttgccc ccttcgcagg gggctttttt tgaaaaacgg ctgcggaatt
    11641 agaccagtta tctcccgaag aaggaaattt ccgcagcgcg tgttttactt ctgcccgtaa
    11701 taagcgttag gaccatgctt acgcatgaag tgtttattca tcaggaagct ggcgatgtga
    11761 ttgagttgtg gattaatact gcgcgcaatc cacgccattt tcgccacttc ttccatcact
    11821 accgcgttat gcaccgcgtc gtgggcatct ttcccccagg cgaacggtcc gtgctgatac
    11881 accacaattc ccggcgtatg cagcggctcg gcgttgccca gcgtttcgat aatcactttg
    11941 ccagtgttca gttcatactc accctgcacc tcttcttcgc ttaatccacg cgtacacggg
    12001 atgtcgccaa agaagtagtc ggcatgcgta gtgcctaacg ccgggatcgc cagccccgcc
    12061 tgcgcccatg cggtagcatg agtggagtgg gtatggacaa tgccgccaag cgacggataa
    12121 cgacggtaga gttcaagatg cgtcgcggtg tcggaagacg gacgatattc cccttccacc
    12181 accttaccgc tcatatcgac caccaccata tcgtctgctt tcatggtttc gtaggcgacg
    12241 ccgctgggct tgatcaccac cagcccgcgt tcgcggtcga tagcgctgac gttgccccag
    12301 gtaaaggtca ccagcccata gcgcggcaga tccatgttgg cttcaaatac ctgctgtttt
    12361 agcttttgca ttatgccgcc tccaccatgc ccgctttcgc catgcgcgct ttcacccaat
    12421 cacgcgcttt cgccacttct gccgccgggt cttccgccgt ttcgctccac atctcaatca
    12481 ggtacggccc gcaatagcca ctctgtttga gcgtttcgaa acaacgttcg aaatccacta
    12541 caccttcgcc aaacggcacg tttttgaaga cgccaggttt ggtgtctttc acatgtaccg
    12601 cgacgatatg cccgattccg gcctgcaact ccatctgcac atcgttgtcc cacgccgaca
    12661 ggttgccgat atccgggtag agctggaacc acgggttgtt gagatagtgc gcgtaaccca
    12721 gcgccttgct gatggagttc atcaacggat aatccatgat ctccatcgcc agcgtcacct
    12781 gcgcgcggct cgccatctca acgctctctt tcaggccgtc acggaaacga cgacgcgttt
    12841 cgttattggc ttcctgatag taaacgtcat agcccgccag ctggatcacg cgaatgccga
    12901 cgtcctgggc gaactggata gctttacgca taatctcccg cccctgcgcc cgcaccgcgt
    12961 catcttcact gcccagcggg aaacgacgat gagcagaaag gcacatggac ggcacgcgca
    13021 cgccggtttc aacaatcgca ttcaccagcg ccagacgctg ctcgcggctc cagtcgaggc
    13081 gcgacaggcg ctcgtcagtt tcatctaccg acatctcgac aaaatcgaag cccaacgttt
    13141 ttgccagtcg caggcgttcc agccagcact ccccggcggg gagcgctttt tcatagatgc
    13201 caagcgggat ttgtttggac aacatatccg ctccttagcc ccacagttcg gcgatggaac
    13261 gtttgaactg acgcgcggct tcaaccggag aagcggcatc acggatgctg cgaccggcga
    13321 taaacacatg aatcggaata cctttgaaca gcggcagatc ttccagcgcc aggccaccgg
    13381 tgacggtgac tttgaagccc atatcggaaa gacgtttgat cgcggtaatg tccgcttcgc
    13441 cccacgctac gcctgcggcc tgcgcgtcac ggctgcggtg ataaaccacc tgctgaatgc
    13501 ctgcgtcacg ccactgctgc gcctgttccc aggtccagta gccggtcaat tcgatctgca
    13561 cgtcgccgtt aaactctttt gctacgtcca gcgcgccttt ggcggtgttg atatccgcac
    13621 agcaaattac cgtcacccag tcagcgttgg cttcgaagca catacgcgaa aggattttgc
    13681 ccgcatcagc aattttagcg tctgccagta cgattttgtg cgggtagagc gctttcaggt
    13741 cacgaaccgc acgcacgcct tcgcccacgc acagaatggt acccacttcg ataatgtcga
    13801 cttcttcggc aatcaggcga gtggtttcgt aggcgctatc catagtctgg ttgtccagcg
    13861 cgacttgcaa catcggtaat gacatcttca attccttctt aagctgccgc gttggtgcgg
    13921 tcaatgagat ccagtacttc ctgctcggta cggcaggcac gtaaacggtc gaaattttct
    13981 tcatcttcaa acaggttgac gatctgcatg atgcccactt cctggtgcgt gttggcatcg
    14041 accgccgcca tggtgatgag gatatccacc gggtcgttat cttcgtggtt gaactccagc
    14101 ggctttttca gcgtcaccag cgcaaaaccg gttttcttaa cgccctcttc cgggcgaccg
    14161 tgcggcattg ccaggcctgg tgcgagcaca aagtacggac cgtgctgttc aaccgcatcc
    14221 agaatcgcct ggtagtaacg cggctcgaca acatccgccg ccaccagcag gtcaacgccg
    14281 atttttactg cgtcctgcca tgtctcagct tcagcctgca ggcggatgga tttattctcc
    14341 gccagcgaat cacgtaattt catggcgcgt ccttacttca catcctgcgg gaaatgcgct
    14401 ttgatcactt ccagcagttt cgggccaaag tcggcaggag agagcatgtt gcgcacgcca
    14461 accacgtatt tgttgccggt cacggtgatt tcgcccgcaa tgtgcgtaga agcgatgatg
    14521 atatccgcgc cgctcaactc gcttttgtat tcgccaaccg cgcagctatt taccgtgtgg
    14581 tcaatgtttg attgggttaa aaactggtcc actttcatct tcatgatcat ggaactgcct
    14641 tgtccgttgc cacacacagc cagaatacgt acggtcataa tcaaaactcc ttattaagca
    14701 gactgttctg ccagttgttt ttctgcatct tcttctgcgc gcagtgcgcg gccagcgaag
    14761 aacatataag ccagtgcaat tacaatgatg acggccataa aggcgatacc gatggagaag
    14821 aagccctgca tcatcggcgg tgccagaatc gaccagtccg ccatgcccat ccaggcgctc
    14881 ataccggtga gtttcaccgc ccagacgcag ccaaagattt caatcatccc catcaccaga
    14941 catatcttca gcgccgcacg ccagccgccg aagtggttag cgaacacgcc gatggtggcg
    15001 ttggagaaga acatcgggat aaagccggga ataatcagga tcgaggaacc gcaggcgacc
    15061 aggatgccaa ccgcaatcag ctgaccgatg gtgccccaca taaagcccca aaccacggcg
    15121 ttcggcgcga agctatagat agccgcacag tcaatcgcca gaaccgcacc agggatcagg
    15181 cgctgagaga taccgttaaa tgcttcagag agttccgcca caaacatgcg cacgccctga
    15241 gtgatgatga agatcgccac cgcgaaggag aaaccagttt gcaggatgta caccgtccag
    15301 ttcactttgc ctgccatcgc ctgcacggtg tcgataccga aggagagcag aatggcacca
    15361 aagaagatgg tcatcacaat cgccgtggag acgatgttgt cgtggaaaat attcagccag
    15421 cctggcagtt tgaggtcttc aacgctttcc tcttttttgc cgaggaacgg cgcaacttta
    15481 taggcaatcc atgatgcaaa ctgttgctgg tgaccgatgg agaaaccgca gccgtccgtc
    15541 acttcctgag tcggcttgta catcatgttg gaggtaatgc cccagtagag cgaaaccaga
    15601 atcgccgtgc agataatggt tgtccacatg gagtagccga aaataaacaa cgtaacggca
    15661 atcagccccg cctgctggaa catgatatga ccagtcagca tgatggtacg aataccggta
    15721 atgcgacgca gcagcacgta acagatgttc agcgccagcg ctaacaacac cgcgtaacct
    15781 acccagctat aggcatcgcc catgcggtcg atggttgcca tcatcgacgc gtaagtatcg
    15841 gaaattgcgc cgttaatgcc gtagacttcg gacattttcg ccaccaccgg tttgaaggtg
    15901 ctggtgagga tgccggaccc tgcctgcaac aacatgaaac caattatggt tttaatcgtg
    15961 cctttaataa taacgctgac acttttgcgc agtaggatgt agcccagaca ggtcacaata
    16021 cccagcaaca acggggcatt ggtcatgacc tggttaaaaa acacggtaaa gatgttgtag
    16081 aggatctcca taacgatctc ctgaagaaga ggtaaccggg tattccacac acccggaatg
    16141 ttgtgcactc actctaattt tcaaaagtaa tcacaacaag attatttatg attaaatgtg
    16201 acgcgacccg caaataattc cacatggata gaatggtatt cacacaacga aacaactatt
    16261 catttaaatt catttagtta attgaattac acattccatt aaatctttcc acactcaaaa
    16321 tcaacaacca cttccctggc aaaaagtcaa aaaatggtca ttgccttcct gaaaaaatag
    16381 tgtcaggatt aatcaaaacc aatcacacat tgattcgaat tgaacaatca tgaaggtaaa
    16441 tggcgatgag taaagtgaaa agtatcaccc gtgaatcctg gatcctgagc actttcccgg
    16501 agtggggtag ctggttgaat gaagaaattg aacaagaaca ggtcgctcca ggcacatttg
    16561 cgatgtggtg gcttggctgc accgggatct ggttgaaatc ggaaggtggc accaacgttt
    16621 gcgttgattt ctggtgcggc actggcaaac aaagtcacgg taacccgtta atgaaacagg
    16681 gtcaccagat gcagcgcatg gctggcgtga aaaaactgca gccaaacctg cgcaccaccc
    16741 cgtttgttct tgatccgttt gcgattcgcc agatcgacgc ggtactggcg actcacgatc
    16801 acaacgatca tatcgacgtt aacgttgctg ctgccgtgat gcagaactgt gctgatgacg
    16861 taccgttcat tggaccgaaa acctgtgtgg atttgtggat tggctggggc gtaccgaaag
    16921 agcgttgcat cgtggtcaaa ccgggcgatg tagtaaaagt gaaagacatt gaaattcatg
    16981 cgcttgacgc tttcgaccgt actgcactga tcaccctgcc tgccgatcaa aaagcggctg
    17041 gcgtactgcc agatggcatg gacgatcgcg cagtaaacta cctgttcaaa acccctggcg
    17101 gttccctata tcacagtggc gattcccact actctaacta ttatgcgaag cacggtaacg
    17161 aacatcagat cgatgtggcg ttaggatcgt atggtgagaa cccgcgcggt attaccgaca
    17221 aaatgaccag cgccgatatg ctgcgtatgg gtgaagccct gaatgcgaaa gtggttatcc
    17281 cgttccacca cgacatctgg tcaaacttcc aggccgatcc gcaagagatc cgcgtgctgt
    17341 gggagatgaa aaaagatcgt ctgaagtatg gcttcaagcc gtttatctgg caggttggcg
    17401 gcaaatttac ctggccgctg gataaagaca acttcgagta ccactatccg cgcggtttcg
    17461 atgattgctt cactattgaa ccggatctgc cgttcaagtc attcctgtaa tctgtcgttt
    17521 ataccggata gcgtggagaa gttcgcgcta tccggcgtag tatttcctgg ctatttcaaa
    17581 tatcatctaa aaaaatcaaa ttttatcgga atagctcatg actgaagcac aaagacatca
    17641 aatcctcctg gaaatgctcg cacaattggg ctttgtgacc gttgagaaag tcgttgagcg
    17701 tctgggaatt tcgcctgcca ctgcgcgacg cgatatcaat aaacttgacg aaagcggcaa
    17761 actgaaaaaa gtgcgcaatg gcgcagaagc tattacccaa cagcgcccgc gctggacgcc
    17821 gatgaatctg catcaggcgc agaatcacga tgaaaaagta cgtatcgcta aagcggcctc
    17881 gcagctggtt aatccgggcg aaagcgtagt catcaactgc ggctccaccg cgtttctgct
    17941 tgggcgggaa atgtgtggca agccagtgca aatcatcact aattatctac cgctggcaaa
    18001 ttacctgatc gatcaagaac atgacagcgt gatcattatg ggcggacagt acaacaaaag
    18061 tcagtccatc actttaagcc cgcagggcag cgaaaacagt ctctatgccg ggcactggat
    18121 gtttaccagc ggcaaaggac tgacagcaga agggttgtat aaaaccgata tgctgacggc
    18181 aatggcagag cagaagatgc tgagcgtggt agggaaactg gtggtactgg ttgatagcag
    18241 taagattggc gaacgcgcgg gaatgctttt tagccgtgcc gatcaaattg atatgctgat
    18301 caccggcaaa aatgctaacc cggaaatcct gcaacaactg gaagcgcaag gggtcagcat
    18361 tctgcgtgtt taaagatgct ggcggaaaaa tgtcactgca gcatctaacg cctcaggcgt
    18421 aatgcggtga cgcacgcctg gctgccatga acaggttagc agtttatccc gtcccgtctc
    18481 gcttaaggcc tgctgcaaac gtagtgattc gtcggcaggc acaacgtcat cgtcgaggcc
    18541 atgccacagc agtagaggtc tgtcactaag ttgttccagg tggtttgtcg cttcccactc
    18601 tgccagcggc gcgacaatgt tattgaattc attctgctgt gctgtcgttt caggtatcag
    18661 cggtggaaac agtgaacggg cgagtgatgt aaaatagccc gatcccatca tgctggcggt
    18721 acatctcacc gtggggtggc gagcggtaat ccccagtgcc gtcatcgcgc ccatcgacgc
    18781 accaccgact gccagacggt catcaagcag ccagttttct tcggctattg ccgcacgtaa
    18841 agtagtgaat tcctgcatac tttgtagcaa gatttgccag aattgattta accgccgcgc
    18901 tgcgtcacca ctaaaacggc taccgtgatc gggcgcatcc ggcatgatca ctcgcaaacc
    18961 agcctgcgcc agcgcaacgg caaaatagct atacaccaga ctggatgaag taaagccgtg
    19021 ataaaaaatt acgcacggta acggggtatc tttttgcccg acaggataag catgaagaac
    19081 gggaatatct gccagctcgc gtgattctat ttcaatcatc tgtctctcct ttttgtctgg
    19141 tatgacatga agaaatcctt agcacaagtt tctgctggcg gtattagttc ataatgttga
    19201 gatgtgggtt acgctttcgt cacgttttca ttcgaaaatc gcgtaccagg taacaattcg
    19261 ggaacattcc cccaaagtta aattaataag gcactacact atggttagca ggaaacgtaa
    19321 tagcgttatt taccggtttg ccagtttatt attggtgttg atgttaagtg cctgtagcgc
    19381 actgcaaggt acgccacagc cagcaccgcc agtaacggac catccgcaag agattcgccg
    19441 cgaccagacg caagggttac agcgaatagg cagcgtaagc acgatggttc ggggttctcc
    19501 ggatgacgca ttagcagaaa tcagagcgaa agctgtcgct gcaaaagctg attattacgt
    19561 tgtcgtcatg gtggacgaaa ccatcgtgac aggacagtgg tattcacaag ccattttgta
    19621 ccgtaaataa atcagacaac ttacatagtc agctttacat tgctttgcgt ccctatgcgt
    19681 ttacctgtgc acaataaatt accggctgga agcctgggcg tttattaccg cgatggaatg
    19741 ccctgcaaca tgtggggaaa cgaaaaatgg agctgacgat gaaacaatta cttgcctcac
    19801 cctcgctgca attagtgact tatcctgcga gcgccacggc acagtctgca gaattcgcca
    19861 gtgctgactg cgtgacgggc ctgaatgaaa taggtcagat ctcagttagc aatatttcag
    19921 gggatccgca ggacgtggaa cgtatcgtgg cgttaaaggc cgatgaacaa ggtgcgtcat
    19981 ggtaccgcat tattaccatg tacgaagacc agcagcctga taactggcgc gtacaggcga
    20041 tactgtatgc atgagtgtca tctacagcaa atgaacaatg ccggatgcgg cgcgagcgcc
    20101 ttgtccggcc tacaattttc acatatttca tttagttaat cgaaaccagc gtcgcatcag
    20161 tcgtatacgc ttacacacac actccccccg tcgcccgcag caataaatca ttctggatct
    20221 gctctgacaa ccgtacgccg ccgcgcgtat ctaacatcac ctgacaccac gcctgcgcca
    20281 ttggcggaga agcatatttc aacatttgcg caccgcagcc cagcaggaat agctgatgag
    20341 taatctctcg cctcagttct tcagctggct tacgcagctg ctgctgtaaa cgacgaaccg
    20401 cgcgatcaaa atagcgatcc tgccctttca cttccacaaa cgcttccgac aataagtcgt
    20461 atacgcccgc ttgcttattg agaacgcgca gcacatccag gcacataata ttgccggaac
    20521 cttcccaaat actgtttacc ggcatctccc ggtaaagccg cggcagctcg ctctcctcgc
    20581 aataaccaat gccgcccagc acctccatcg cttcggccac aaacggcata cctcttttgc
    20641 agatcacaaa ttttgccgca ggcgtaaata aacgcgccca cagggcttct ttggcatcgg
    20701 cacgccggtc ccacgctcgc gcaagacgaa acagcaacgc cgtttgccct tcaagctgaa
    20761 gtgccatgcg acttaagaca tgacgcataa ggggctgttg gatcaatgga ttaccaaaaa
    20821 catggcgttg atgtgcatga taaatcgcca gcgaaaatgc acggcgcatc atggcatggc
    20881 tacccagggc gcaatcaaaa cgcgtcatcc cacccatttt caggatcaga cgaattcctt
    20941 ccccttcctg ccccaacaac cagccaatgg catcctgaaa ctccacttcg cagctggcgt
    21001 tagagcgatt acccagctta tctttcagcc gctcgaggcg aatcgcgttg cgttgcccgt
    21061 caggcaaaaa gcgcggcaca aaaaagcagg acaaaccgcc cgtagtctgc gccagcacca
    21121 gatgcgcatc gctttgcggc accgagaaaa accatttatg ccccaccagc cgataagagc
    21181 catcttccag gcgctctgcg cgggtggtgt tgctcatgac atcggaaccg ccctgctttt
    21241 ccgtcattcc catgccaatc aacaaaccgc gtttttgccc acctggcaat aagtgagaat
    21301 cgtagcgatc gctcagcagc ggcgtggtcc agtcctgaaa cggcgcgggt aacatctgta
    21361 acagcaatgg cgtggcggca aaggtcatgg ttatcggaca taacgaccct gcctcaacct
    21421 gtgcgtgcaa cataaaacgc gccgcgcgcg ccacaaatgc gccggagcga gcgtcttctt
    21481 cccaggcaag attgtgcacc cgattggtac atagcacctg catcagcagg tgccaggcgg
    21541 ggtgaaaacg cacatcgtcc agacggcgtc cttgcgcatc gtagcgcaat aattcaggcg
    21601 gattcacatt cgccagccgc cccagttcaa gggattcagc cgttcctaac tgctgaccaa
    21661 tactggctag aaaatcgcta tcccagccag caccttcacg cgttaccgct tcgcagagcg
    21721 cgccatcaga caggtataag ttgctgttat ttaatggtat aggttgatta aaaacggtgt
    21781 gagtttgcca gtgcactgtg tctccctcca tcaatggcag tcaccattag tatggtcact
    21841 gccatggatt catgacagaa agagattgct aaaacattct tatccagcaa tgtaatgcgg
    21901 aacgaaacgt gaggtgtctt tggtgatcag tgtgttatct tcacggatcc ccattccgca
    21961 cgcttcatca tcgacaatcc agctaccgat gagtgtgtag ctatcgccaa atcgcggcag
    22021 aggttgaaac gcctggtaga tcatcggttc atcggcgtaa tcaccatcag cgtggtcaac
    22081 gacgttattc tgaccgtcga aaatggtgac gtttccgcct tcgcgcgagt agatcggttt
    22141 acgcacatag ctttcgccag cggcaatctg cgatttttca ccctcaaacc aggatgcaag
    22201 aaggttagga tgaccaggga agaagcgcca aagcagcggc attagccctt tattactcaa
    22261 gatacttttc cataacggct ccacccattg ctcgcgacgc ttgcacagca gcggaccgtt
    22321 atcgtcacgc atcatccact ccagcggata cagcttaaat gcacgctgga tgacattatc
    22381 atcaagatcg gtcagtacac cgccgacgcc caaaccgaga tcttcaatgt agataaaccg
    22441 cgactcctgc cctgcctgct gggcgcagtc ctgcaagtac agcacggtag ccctgtcttc
    22501 atcggtgtcc tgacagcagc agaaataaaa cggctcccgg ctataaagct cgctaaagcg
    22561 tgaaatcagg cgttcctgaa tggcattgta ctgatcggca tcacgcggaa taatgccgct
    22621 gcgccgggca tcctccagcc acaaccactg gaaataagcc gactcataca acgaggtcgg
    22681 cgtatcggcg ttgtactcca gcagcttcac cggcgcattg ccacaccagg caaaatccat
    22741 ccggccatac agcgaaggat cgcgtgcgcg ccagctttcg gcgatcacat cccagtacaa
    22801 cggcgggatc gccagttgcg tcaggatctc ttcgtcttta accgcgcgat ccaccacctc
    22861 aaggcacatc tgatgcagtt ccgcagtcgg tttttcgatc tgctcttcaa tctggcgcag
    22921 agtaaaacgg taagcccgac tctcgtccca atagatttca ttgtcgatga tatgaaagtc
    22981 gaaaccgttg ttagcggcga tctggtccag atcccgtcgc acaggaacgt tgtgtctcag
    23041 catgattagc ctccccaatg cccacgggcg ctagaagaac gaccatagcc gccgcgcgaa
    23101 acggttgacg cttttttggt ggtaaagccg cctgaagagt aagactcttt tttgccagaa
    23161 ccggagcgcc aggagtaatc accggaagtg ttgcgccaga cggggcggga agcaaatgag
    23221 gatccgccgc tgttatagac gaacggctca tcgcgatctt tacgaataac tcggcttaac
    23281 aaaaatccag aaacgaccgg gatccaactc tgttcaacat tgtcgtagta gcaattttcg
    23341 tacttagact gacaattctg ctgagtcata ttcttcggaa catcggcata aaatgccgct
    23401 ttggcgttgt tccagccacg cgcgcaaatg tcggcattat taccgtcatc aatacaatcc
    23461 tgcactgtcg aataaaacgt tccgtcgccg tcgttatcga catcgccact ttcactccat
    23521 cctttgacta ttaaaaaaac ggcaccgccc attagcacca acgttaagta tttttgtgcg
    23581 tagcgattgc ggcgcggctc aaaaggatta tgcggtcttc caatgcgact gatcgctccg
    23641 tgaccaattt tactgttatt tcttgatttg cgttttctgg ccatgatcgt ccattaccat
    23701 gtcatacaag ctgcgttaaa aataccacca gccagtgcgg cggttcccat aaacattcct
    23761 gctgcggtat tgtgattaat aattttttcg cttaatgcgg gcatataaag tctgacacca
    23821 gcaaaaacga gtaactgaat caccagtgca atcccgcccc aggcgaaata gtctggaata
    23881 ctcaccgcat tgattgccgc actggataag gggatcacgt aacccaacaa tgtaccgctg
    23941 aatgccagtg acgccgcggt attattgttt ttgattaatt gccattcgtt gtgcggtgta
    24001 attttggagt agataaacag aaaaataatc accatagcca cgccaataaa aaaataggca
    24061 ctaaaggcaa gaagtgaatc cagtatatgc attgtaaacg tccttgtaat taaccaataa
    24121 tatgaagtga tgtcagcggg atatcgacac ccaacgcgcg cgaaaataac cactctggct
    24181 cgccgagatc gttaaaagat tcctcaccat ttaacagcaa atattcgtaa gtatcttcgg
    24241 ttacttggcg ttggtagccc atggtaaaat tatggacctc ccatttggca tggttttgat
    24301 tttctacttt ttccagcatg tagacgggtt cgatatttcc cggttcttcg ctgttaaaaa
    24361 atcgctgcca gcgtttttcc tgccagttta agatcattgc ccccatcact ttggcgttga
    24421 tcgcctcgcg ccagtgactc tctttactga taccgtagct ctcttcatag acaaagagct
    24481 tgatatcatc aatgtcatca atatcttcgc cgccggtggt attgatttgc agaaattcat
    24541 cgcctgaagt gtagtagcgg aaaatctgac taccgccgcc cagatcgatg tggctgacag
    24601 cggctaccgt aaattcctca cccggcagcg caatcagcaa ttcatcttcc agcaaacgaa
    24661 acgctaacgt gtcgagcgta aagccactat tgagatgaag tcccagcgga ccgcgagcga
    24721 tagcaggctt attatccttg ccaaacagac gctggaaaaa accagacata ttttatcctt
    24781 aaaatagttg ccagcctttt agcgaggctg gcaaaaaaat tactcgccct gttggcgttg
    24841 cagtcttgct aatacatcct gggcgctact tttattgcta ccgccaattc cggcttccgc
    24901 cagcttttca tcaagatcgc gaccgtctgc gactttttcc aactgtgcag cagcatccag
    24961 gcgagcctga cgttcggcct gacgcgtttg caggcgtttt aaggattctg ccgctgtcga
    25021 aacgctggaa gatgcgccaa cggtagaggt tgttacagcc tgttgtgcac gctgcatggc
    25081 ttcagtggct ttaacgactt ccatttgctg ctcaaactga gcgatacgct gcgctgtcgc
    25141 tgtaactgct ttttccacgc catcacggga gacttccaga ttcgacaaca cttgctcttc
    25201 agcggtaatg agattctcaa ggcgcgcgat ttcttcagca acttcgttaa tcaacgacgg
    25261 attaacgttc ttgcttaacg cttccagcgc acgggcttcc agactggctt tgcgctcacg
    25321 taaatctttc agcttatcgt gacttaattt cacccgcgcc agcagatcaa cgcgagattt
    25381 tccggcttta tcgagttcag ctttagcgtc acgaatatgc tgttccagca tgcgcacgcc
    25441 ctgggtttct tcaatggatt cttccgcctg ggagataaac gatttcccca gcgtaaataa
    25501 actttttaaa attcccataa atacccgtcc ttgtaattag tgtgaatatt cttctgtaat
    25561 ttcagccaga tccaatgcgt tatctaccag cgaggttatc tcaagcagga tatcttcaag
    25621 agaagattta agcgataacg ccccgaaaac aatgtaatac tcttcctgtt gcacgctgga
    25681 gattcctacc gatgacaacg gcatcatctt ctgatttctt aataagaagg tattaaattc
    25741 atctggattg ctgatactac ttaccggaca aataaaggtt tcgataatca tttggcggga
    25801 agtaaaaaga atattgattt gcaaatcgcc ataatcattc attttaataa ttaa
//
LOCUS       DAZGVQ010000066        25826 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000066 DAZGVQ010000000
VERSION     DAZGVQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.066"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            28..2256
                     /gene="crfC"
                     /locus_tag="ACHLAG_002586"
     CDS             28..2256
                     /gene="crfC"
                     /locus_tag="ACHLAG_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFP0655792.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRFENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSAVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2253..3131
                     /gene="rdcB"
                     /locus_tag="ACHLAG_002587"
     CDS             2253..3131
                     /gene="rdcB"
                     /locus_tag="ACHLAG_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFP0655793.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGVFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            3395..4897
                     /gene="proP"
                     /locus_tag="ACHLAG_002588"
     CDS             3395..4897
                     /gene="proP"
                     /locus_tag="ACHLAG_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFP0655794.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5009..5098
                     /gene="pmrR"
                     /locus_tag="ACHLAG_002589"
     CDS             5009..5098
                     /gene="pmrR"
                     /locus_tag="ACHLAG_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFP0655795.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFASF"
     gene            complement(5074..6174)
                     /gene="pmrB"
                     /locus_tag="ACHLAG_002590"
     CDS             complement(5074..6174)
                     /gene="pmrB"
                     /locus_tag="ACHLAG_002590"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFP0655796.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNEANQI"
     gene            complement(6175..6843)
                     /gene="basR"
                     /locus_tag="ACHLAG_002591"
     CDS             complement(6175..6843)
                     /gene="basR"
                     /locus_tag="ACHLAG_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFP0655797.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6840..8486)
                     /gene="eptA"
                     /locus_tag="ACHLAG_002592"
     CDS             complement(6840..8486)
                     /gene="eptA"
                     /locus_tag="ACHLAG_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFP0655798.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFLRGA
                     NILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLV
                     RIGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNT
                     ASCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGAC
                     DRVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYN
                     RYPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTS
                     LVYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQA
                     QTQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            complement(8590..9927)
                     /gene="adiC"
                     /locus_tag="ACHLAG_002593"
     CDS             complement(8590..9927)
                     /gene="adiC"
                     /locus_tag="ACHLAG_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFP0655799.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10064..10825)
                     /gene="adiY"
                     /locus_tag="ACHLAG_002594"
     CDS             complement(10064..10825)
                     /gene="adiY"
                     /locus_tag="ACHLAG_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFP0655800.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11150..13417)
                     /gene="adiA"
                     /locus_tag="ACHLAG_002595"
     CDS             complement(11150..13417)
                     /gene="adiA"
                     /locus_tag="ACHLAG_002595"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFP0655801.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEVTPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(13616..14524)
                     /gene="melR"
                     /locus_tag="ACHLAG_002596"
     CDS             complement(13616..14524)
                     /gene="melR"
                     /locus_tag="ACHLAG_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFP0655802.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14807..16162
                     /gene="melA"
                     /locus_tag="ACHLAG_002597"
     CDS             14807..16162
                     /gene="melA"
                     /locus_tag="ACHLAG_002597"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP0655803.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            16265..17686
                     /gene="melB"
                     /locus_tag="ACHLAG_002598"
     CDS             16265..17686
                     /gene="melB"
                     /locus_tag="ACHLAG_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFP0655804.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(17825..18454)
                     /gene="yjdF"
                     /locus_tag="ACHLAG_002599"
     CDS             complement(17825..18454)
                     /gene="yjdF"
                     /locus_tag="ACHLAG_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFP0655805.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(18577..20223)
                     /gene="fumB"
                     /locus_tag="ACHLAG_002600"
     CDS             complement(18577..20223)
                     /gene="fumB"
                     /locus_tag="ACHLAG_002600"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP0655806.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRKGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(20301..21641)
                     /gene="dcuB"
                     /locus_tag="ACHLAG_002601"
     CDS             complement(20301..21641)
                     /gene="dcuB"
                     /locus_tag="ACHLAG_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFP0655807.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(22212..22931)
                     /gene="dcuR"
                     /locus_tag="ACHLAG_002602"
     CDS             complement(22212..22931)
                     /gene="dcuR"
                     /locus_tag="ACHLAG_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFP0655808.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEMIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(22928..24559)
                     /gene="dcuS"
                     /locus_tag="ACHLAG_002603"
     CDS             complement(22928..24559)
                     /gene="dcuS"
                     /locus_tag="ACHLAG_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP0655809.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLAYKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            24740..24970
                     /gene="yjdI"
                     /locus_tag="ACHLAG_002604"
     CDS             24740..24970
                     /gene="yjdI"
                     /locus_tag="ACHLAG_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFP0655810.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            24982..25254
                     /gene="yjdJ"
                     /locus_tag="ACHLAG_002605"
     CDS             24982..25254
                     /gene="yjdJ"
                     /locus_tag="ACHLAG_002605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0655811.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            25481..25777
                     /gene="ghoS"
                     /locus_tag="ACHLAG_002606"
     CDS             25481..25777
                     /gene="ghoS"
                     /locus_tag="ACHLAG_002606"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP0655812.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
BASE COUNT         6271 a         6223 c         6703 g         6629 t
ORIGIN      
        1 aaaatttgct ttaaggaaga attttctatg tacacacaga ccctgtatga gttaagccag
       61 gaggctgaac gcctgttaca gctttctcgc caacagttgc agttactgga aaaaatgcct
      121 ctctctgtac ccggagacga cgcgccacaa ctggctttac cctggagtca gcctaatatc
      181 gccgaacgtc acgcgatgct gaataatgag ttgcgtaaaa tttcccgact ggaaatggtg
      241 ctggcaattg tcggtaccat gaaagcaggg aaatcaacca ccattaatgc cattgttggt
      301 acggaggttc tgcctaatcg taatcgccca atgactgcgc tgccgacgct tattcgccat
      361 acgcccgggc aaaaggaacc ggtactgcat ttttcacatg tcgcgccaat cgattgttta
      421 attcaacaat tacaacagcg cctgcgtgat tgcgatatta agcatctgac cgatgtgctg
      481 gaaatagata aagatatgcg tgcgcttatg cagcggttcg aaaatggcgt cgctttcgaa
      541 aaatattatc tgggtgccca gcctattttt cattgtctga aaagtttgaa tgatttagtg
      601 cgactggcga aggcgctgga cgtcgatttt cctttttctg cttacgccgc cattgagcat
      661 attcccgtga ttgaagtgga gtttgtccat ctggcggggc tggagagtta tcccggtcag
      721 ttgacgttac tggatacccc cgggccaaat gaagccgggc aaccgcatct gcaaaaaatg
      781 cttaaccagc agctggcacg cgcctcggcg gtactggcgg tgctggatta tacgcaactg
      841 aaatcgatct ccgatgaaga ggtccgtgag gcgattttgg cggtggggca atcggtgccg
      901 ctgtatgtgc tggtcaataa gttcgatcaa caggatcgta acagtgacga cgccgaccag
      961 gtgcgggcac tgatttccgg gacgctgatg aaaggctgta ttacgccaca gcagatattt
     1021 ccggtgtcgt cgatgtgggg ctacctggcg aatcgggcgc gccatgagtt agccaacaac
     1081 ggtaagttac cagcgccaga gcaacaacgc tgggtggaag attttgccca tgccgcgctc
     1141 ggcaggcgct ggcgtcatgc cgatctggcg gacctcgaac atattcgtca tgctgccgat
     1201 cagttgtggg aagattcgct gttcgcccag ccaattcagg cgttgcttca tgccgcttac
     1261 gctaacgcct cgttgtatgc tttgcgatct gctgcgcata aactgttgaa ttacgcgcag
     1321 caggcgcggg aatacctgga ttttcgtgcg cacgggttaa acgtcgcttg tgaacaattg
     1381 cggcaaaata tccatcaggt cgaagaaagt ttgcagctat tgcaactcaa tcaggcgcag
     1441 gtgagcggcg agattaaaca tgaaatcgag ctggccctga cctccgccaa ccactttctg
     1501 cgtcaacaac aagatgcgct gaatgcgcag ttagccgcct tgtttcagga tgattcggag
     1561 ccgttaagcg agatgcgtac ccgctgtgag acactgttac agacggcgca gaacaccatc
     1621 agtcgcgact ttacgctgcg ttttgccgag ctggaatcca ccctttgccg ggtgttaacc
     1681 gatgttattc gccccattga gcaacaagtc aaaatggaat tgagcgagtc agggtttcgt
     1741 cctgggtttc attttcctgt ttttcacggc gtagttcccc acttcaacac tcgccagctg
     1801 ttcagtgcag tcatttcgcg ccaggatgca acggacgagc agagcacgcg tttaggcgtt
     1861 gtgcgtgaga ctttttcgcg ctggttgaat cagcccgact ggggacgggg aaatgagaaa
     1921 tccccgacag aaacggttga ttacagtgtg ttgcaacgag cattaagcgc agaagtcgat
     1981 ctttattgcc aacaaatggc taaagttctg gcagagcagg tcgatgaatc tgttacggca
     2041 ggcatgaata cttttttcgc tgagttcgct tcatgtttga cggaattaca gacgcgttta
     2101 cgcgaaagcc tggctctgcg tcaacaaaat gaatcggtgg tcaggctgat gcagcagcaa
     2161 ttgcagcaga ctgtgatgac tcacggctgg atttacaccg acgcccagct gttacgcgat
     2221 gatattcaaa cacttttcac ggcagaacga tattgaccaa gacgttactg gacggccccg
     2281 gtcgcgtgct ggagtcggtt tatccccgct ttttagtgga tctggcgcag ggcgatgatg
     2341 cccgccttcc acaagcccat cagcagcagt ttcgtgaacg actgatgcag gaacttcttt
     2401 cgcgtgtgca gcttcagaca tggacgaacg gcggcatgtt aaatgcgccg cttagcctgc
     2461 gactgacaat ggtggaaaaa ctggcgtcga tgctggatcc cggtcatctg gcgctgacgc
     2521 agatcgcgca gcatctggcg ctgctgcaaa aaatggatca ccgccagcac tctgctttcc
     2581 cggagctccc ccagcaaatt gccgccttgt atgagtggtt ttcagcccgt tgtcgctgga
     2641 aggaaaaggc attaacgcaa cgaggcctac tggtgcaggc gggtgaacag agcgagcaaa
     2701 tttttacccg ctggcgtgct ggggcgtata acgcctggtc gttgcctggg cgctgtttta
     2761 tcgttctcga agagttgcgt tggggggtat ttggcgatgc ctgtcgtctg ggaagcccgc
     2821 aagcggtggc gttgttgctg ggtgatttgc gcgtgaaagc gacacaacat ctggcagaga
     2881 gtatcaatgc ggccccgacc acgcgtcact attaccatca gtggtttgcc tcttcgaccg
     2941 ttccgacggg cggggatcat gctgattttt taagttggct gggaaagtgg accacggcag
     3001 ataaacaacc cgtttgctgg tcagtgaccc aacgctggca aactgtcgcg ctggggatgc
     3061 cacgactctg ttcagcgcag cgtctggcgg gggcaatggt cgaggaaatc ttctctgtaa
     3121 atttggcgta aataatcagt tacatcaatg agtcctaaac gaaatccatg tgtgaagttg
     3181 atcacaaatt taaacactgg tagggtaaaa aggtcattaa ctgcccaatt caggcgtcaa
     3241 ctggtttgat tgtacattcc ttaaccggag ggtgtaagca aacccgctac gcttgttaca
     3301 gagattgcat cctgcaattc ccgctcccct tttgcggccg tcgcgctgat ttttctggcg
     3361 tttgcggaaa tgggccaact ctgcgaggaa agctatgctg aaaaggaaaa aagtaaaacc
     3421 gattaccctt cgtgatgtca ccattattga tgacggtaaa ctgcgtaaag ccattaccgc
     3481 agcatcactg ggtaatgcaa tggaatggtt tgattttggt gtttatggtt ttgttgctta
     3541 cgcattaggt aaagtttttt tcccgggggc tgaccccagc gtgcagatgg ttgctgcact
     3601 tgccacgttc tccgttccct ttctgattcg accgcttggc gggctcttct ttggtatgtt
     3661 gggcgataaa tatggtcgcc agaagatcct cgctatcact attgtgatta tgtcgatcag
     3721 tacgttctgt attggcttaa taccgtccta cgacacgatt ggtatttggg caccgattct
     3781 gctgttgatc tgtaagatgg cacaaggttt ctcggtcggc ggtgaatata ccggggcgtc
     3841 gatatttgtt gcggaatact cacctgaccg taaacgtggc tttatgggca gctggctgga
     3901 cttcggttct attgccgggt ttgtgctggg tgctggcgta gtggtgttaa tttcgaccat
     3961 tgtcggcgaa gcgaacttcc tcgactgggg ctggcgtatt ccgttcttta ttgctctgcc
     4021 gttagggatt atcgggcttt acctgcgcca tgcgctggaa gaaactccgg cgttccagca
     4081 gcatgtcgat aaactggaac agggcgaccg cgaaggtttg caggatggcc cgaaagtctc
     4141 gtttaaagag attgccacca aacactggcg cagcctgttg acatgtattg gtctggtaat
     4201 tgccaccaac gtgacttact acatgttgct gacctatatg ccgagttatt tgtcgcataa
     4261 cctgcattac tccgaagacc acggggtgct gattattatc gccattatga tcggtatgct
     4321 gtttgtccag ccggtgatgg gcttgctgag tgaccgtttt ggccgtcgtc cgtttgtgct
     4381 acttggtagt gttgccctgt ttgtgttggc gatcccggcg tttattctga ttaacagtaa
     4441 cgtcatcggc ctgatttttg ccgggttact gatgctggcg gtgatcctta actgctttac
     4501 gggcgttatg gcttctacct tgccagcgat gttcccgacg catatccgtt acagcgcgct
     4561 ggcggcggca tttaatattt cggtgctggt tgccggtctg acgccaacac tggcggcctg
     4621 gctggtcgaa agctcgcaga atctgatgat gcctgcctat tacctgatgg tagtggcggt
     4681 gattggttta atcaccggcg taaccatgaa agagacggca aatcgtccgt tgaaaggtgc
     4741 gacaccggcg gcgtcagata tacaggaagc gaaggaaatt ctcgtcgagc attacgataa
     4801 tatcgagcag aaaatcgata atattgacca cgagattgcc gatttgcagg cgaaacgtac
     4861 ccgcctggtg cagcaacatc cgcgaattga tgaataagct gaaacggatg gcctgatgtg
     4921 acgctgtctt atcaggccaa ttgaactctt aaggttcact taatctctga cgcgcatact
     4981 ctcctccagg ttaacggagg agagtgcaat gaaaaaccgt gtttatgaaa gtttaactac
     5041 cgtgttcagc gtgctggtgg tcagcagctt tctttatatc tggtttgcct cgttctgatc
     5101 tttcttcagc cgtacccagg cccgcgtgcc ggaagtctct tgccggtttt gcaggaaaaa
     5161 ctgcccgtga tgcaactgtg taatgcggct gacaatactt aaccccagac caatcccgcc
     5221 ataacggctg tccatacgta caaacgcttt actcaactcc ccgcatttac tctcatcaat
     5281 acctggtcct tcatcttcaa ctgccatgac cgctccgcca tcttcttgca gcttaatcat
     5341 aatgttgctg ccttgcgggc tgtaacgatg ggcgttttct accaggtttc gcaataacat
     5401 ccgcagcagg gttgcatcac cctgaacggt gatgtcggcg gcgctctctg gcaatagcag
     5461 ggtttgctgt cgctggtcga gcatggtact gagttcgtca tacgagggga gaatgacatc
     5521 ttccagcagt tttacatgtt gataattacc ggaagaaaat gactgtccgg cacgcgccag
     5581 ttgcagcagc tgggagacgc tctccatcat ctgatcaagc cgtgccacta acggtgctac
     5641 atcaatgtga tgcgttttcg ccagcagttc cagatgcaaa cgcacccccg ccagtggcgt
     5701 tcgcagttcg tgcgcgacgt cagcggtaaa caacctttcg ttatccagcg tgctggtcag
     5761 gcgactgacc agatcgttca acgccgaaac caccgcttcg atttcgaggg tggcgctgtg
     5821 aatggcaatg ggcgttaagt tgtcggcggt gcgcgcttcc agctcttttt gcagctccgc
     5881 cagcgggcga gtgatgcggc gtaccgcctg atagcagata aatagcgtca ggctgaccat
     5941 aaagacgccg gggacaatca ggctggcgac cgcctcgcgg atctcacgca tgatgtggcg
     6001 atcgttgttg cgattgtcgc gtagcgcctg ctcaaacagc tgaatctgct cggtactttc
     6061 atgccatagc cagaagacgc tgatcagctc aaacaccaac aaaatggccc cgatggtcaa
     6121 tatcagccgt tggcgcagcg atattggtcg gcgcagaaaa tgcatcagat tcaattagtt
     6181 ttcctcattc gcgaccagca tatagccaaa gccgcgcacg gtgcggatac gggctttgcc
     6241 cactttgtcg cgcagattgt ggatatgcac ttccagggtg ttggtcgagg gttcattgtc
     6301 ccagttatag atgtcgttgt agagaatttc ccgatgcacc ggactgcctg ctttgagcat
     6361 taaccgtgac agcagagcat attctttggg cgtcagaatc aactcttcac cgcccatcca
     6421 tacctgacgg cgacccatat tcagcgtcag attgccaaca atcagctcac tttcgccctg
     6481 attattatgg cgtcgtagca gggcgcggat acgggcgtgt aactcttcca gcgcaaaagg
     6541 cttcaccaga tagtcatcgg caccgacatc cagcccagcg attttgtcgg tcagcgtatc
     6601 gcgagcggtg aggatcagta ctggcagggt gtattttttc tgccggatac gggcgagaaa
     6661 atgcagtcca tcttcgtcgg gtaaccctaa atccagtacc accaggctgt aatgaccggc
     6721 ctcaaggctt tgttccgcca tccgcgcggt tgtcacgcca tcgcacgcgt agccttcagt
     6781 ttgcgccgcc agaatcaatc cctgcaataa cagcgtatcg tcttcaacaa tcagaatttt
     6841 cattcactca ctctcctgca agtttgcaga atatcatccg cagcctggta atacttcgtc
     6901 tcaacgccag ttaatcccaa tagcgtggag aataaattgt cttgtgaata gtgttgcgtt
     6961 tgcgcctgtt tttgcaggca gttctggtca acctgatacc gtttttgata atcctccgac
     7021 agccacagca gcatcggcac ctgtttttgg ctatccgggg cgatggcata aggcagaccg
     7081 tgcagataga tgccattttc acctaacgat tcaccgtggt cagaaagata aaccaggctg
     7141 gtggtaaatt tatcctgatg ttctttcagc agattaatcg ctttatcaac aatatagtcg
     7201 acgtaaacca gcgtgttgtc gtaagtgttc accagttgct ctttgctaca ggtctggatc
     7261 tcattggtgt cgcaggttgg ggtaaatttc ctgaactgcg gcggatagcg gttgtaatag
     7321 gtcggaccgt ggctgccgat ggtgtgtaag acaatcacgc catcaccctg caggttattg
     7381 atgtactctt ccagcccgtg gaacagcact tcgtcatagc attcgccgtt gatgcactga
     7441 ccaggcagat tcagcgcggt gacgttctgg tgaggcacgc ggtcgcaggc acctttacag
     7501 ccgccatcgt tgtcattcca cagcacgttg atgcccgctc gctgaatgat atccagcacg
     7561 ccttcctggt gctgtgccag ctcttctttg taatgctcac gcggcatatc cgagaacatg
     7621 cacggtactg aaactgccgt tgccgtgccg caagatgcgg tattagggaa atagaccacg
     7681 ttatctttcg ccagccgcgg gttagtttca cgcgggtagc cgttgaggga gaagttctcc
     7741 gcccgcgagg tttcgccgac aatcaggatg gtcaaatttt tacgtttttc gttctgcatt
     7801 aacgggttgc ggtgggcgtc ttcaccaatt cgcaccagcg gtagatttgc cagtcgctga
     7861 tgggagtacc atgaccagct ggcaacaatg ctgttagagg ggcttaagga tttcaccagc
     7921 tctttgttat tgcggaacaa cgaggcgtag tctttataaa acagtgcggc gaccagcaaa
     7981 atcagtagta cagaaaccag aatattggct ccacggagga aaagaacact gcgcagacgc
     8041 gaggtggcag gtttgatttt tatccagcag gcaatcagcg cagcaagcac gccgctgaat
     8101 cccagcgtta ataacatttg cggtgtcatc agcgcataac tttctgccgg agtggtatca
     8161 ataatattgg caatcatcga gcggtcgatg acgatgccgt aagtcattat gaaatattgt
     8221 gcagccgcgc caaccagaat aaacaggcag gccagtggtc gattaagcca taagaaagag
     8281 cttagtgtca ggacaatatt aatcacgctg aaagcgacga ccggcatcga caagaaaacc
     8341 agtacgttat gcagcgaatc cagcggcagc gcctgcaaca cctgtttaaa aaaggcaata
     8401 ttcaggcaga tagagatata aaaagcggcc aacaatagcc aggcgagtaa attcaaagag
     8461 ggtcttttta gtaggcgctt caacatcacg gtgtttccat cgaacaaagt gcgcatatgc
     8521 tcgcaaagca aaattaagcc aaccttaagt tcttaaggtt ggcttttatg tttgctggat
     8581 taaggggaat taatctttgc ttattggtgc atctaaggga tacgggtttt tatgcagccg
     8641 gttgtaattc agcgcataca tggcggtgat taccatcagg gtgacaaatg accacataac
     8701 ctctttcgcc ccggacccca ccacggccca aatgcagtag aggaaggcaa tggtagtaac
     8761 agccagatat gccgggcgtg ctttaccaaa gtgaccgtga ccgagcagca gtaacgccgc
     8821 acaggtgtaa agatatggca ccagtgtaaa gatgaccgag acggaagaaa ccagaccgaa
     8881 ctctttggtc gcgtttggtg aaatgctgct gagctggaag atggtcatca aaataccgac
     8941 gataatcagc cccgccactg gcgtgcccgc tttatttaca cgggcaaaaa tcggtgggaa
     9001 cagtccgtca tcggcagcgg ctttcgccgt ttgacccgcc agcaacgtcc agccgcccag
     9061 tgaacctaag caacccgcag ctgcgcagaa ggaaacaatg gccccggcgg tgtcacccaa
     9121 cgccatccgt gcggcatcac cgaatggcga agcagaaacg cgcagtgcgg cattagggat
     9181 catccccata atcgcggtgg tagaaagtac atagcaaacg gcggcaatca ataccccacc
     9241 aatggtggcg atagggacat tgcgtttcgg gtttttcacc acacctgcgg caacggaggc
     9301 actttccaca ccgatgaacg accacagcgt aacgttaagg gtactttgaa ttgcaccgaa
     9361 ggtgcccagg ccgctgacgt tccatgccgc catataggtt tcaccacgga accagaacca
     9421 gccaaatacg gcaatcccga cgatggggat cagcgccagc acggtggcaa ctgcctgcac
     9481 acgggtgatc attttcggac cgacaatgtt cagcaggacg aagatccaca gcaccacgac
     9541 gcaggtgatg gttaatacca atggatcttt cagaatcggg aagaagtaac ttaaatatcc
     9601 tacgccaatg accaccatgg cgatattgcc gatccagcag gccagccagt agaggacgtt
     9661 ggtttgataa ccgagaaacg ggccaaagca gcggcgggcg taagcgtaag aaccaccagg
     9721 acttggatcg aggaacgaca ttttggcgta taccatcgag agccccagcg caccgataat
     9781 cgtcaccaac catccataaa tggcaatccc gccagtggag gccaggtttg caggtagcag
     9841 aaaaacacct gaccccataa tattccccga caccatcagg gtgacgggga ttaagcccac
     9901 tttgtgagca tcagcatccg aagacataat taaactcctg cgaaggcgag cttcgtgaca
     9961 ataaattacg tcatatcata cgcctgcatt cgatagttga attattatcg accgggttat
    10021 cgctgatgcc gttattaatg tagctcttcg ttaaggttaa aaatcaggcg acagtgcgtt
    10081 ctctgtgctg actaacataa tgcagcggcg tcataccgta gaagtcttta aagacagaaa
    10141 taaagtacga cgtactgttg tagccgcagg actgtgatac ctgagagata tttttaccgt
    10201 ccatcattaa ttcatttacg gcataacgca tgcggcaggt ggtgattatt tggctataac
    10261 tggtgttttc gcttttcaac tttttcttta acagacttgg gctaagacat aaacaactgg
    10321 ctaccatact aagattccag tctttgtgaa tatcgctttc aataatttga taaacgctgt
    10381 cacttacaca attacgtaac atatacatca ttaatgaaag gaatttttta ctgtcgagaa
    10441 aacgggatag cacagtgaat aataatgcgc gtgttcgttc cttttctgac tcagtttctg
    10501 cgggcatcat gctgtgttgc gcggcaacgc gaaagacgtc tggcgtcagg catggcaggg
    10561 tgagtatggg cgtagcgcta cgttgccata ctggtatttg cgagagatct ttattcagaa
    10621 accggaggta atctttgatg atgtcgtgac taatatgagc aaccaaagtg ttgttaagag
    10681 aggagacgtc gataatatta ttttcacaat ttaataacgc catatggtta gctttaaggc
    10741 taataggttc tttcccattc acccttatcc agacatcttt ttcagtcaat aaaacaatac
    10801 aaggttggtc gctgcaaatc ctcatacatg tactcctgag tgcgaataaa agaccagttc
    10861 tcgctaaagc aaagcgatac cgggaaatgg ttatataatt gtatgatgaa tataaacatg
    10921 cataaaaata acgagtttta ctaatattgc aaatttttat agtaatttct catgtacata
    10981 tttttttaca ggataaataa caggcaaaaa aaagcgcggg gtggggccgc gcaaaaagta
    11041 tgacattttt gtttgcagca atttttatga aaacgttgcc atactcaaaa ggcctaaggc
    11101 caggcggcgt aatgttattt aaacaattac gccttcagcg gaatagtggt tacgctttca
    11161 cgcacataac gtggtaaata ccgtcaataa tttcagtccc ttcagtttcg tgttcaaatc
    11221 cagggaaatg gtggtcccag gattgcagcg agcgtaaata acttacttgc ggactgtttt
    11281 tatcgccgaa gttttcacca gacagcagca tcgggattcc tggcggatac gggataactg
    11341 agtttgccgc gatgcgtcct ggcagatttt caatggatac cagttcgaca ttgttgtcga
    11401 caatcgcgtt gtacgcttca cgcggggtga cttccgccac cggcaggccg gaataggctt
    11461 cgttcaaccg tgcgccaggg ttgttttctt tcagccaggc aaacatggtg tcacccagat
    11521 cgtgaatccc catgttcgcg taagtgtcag gatattgttc aacaagttcc ggcatcacct
    11581 gcgccagcgg tgtgttggcg tcatagtggc gtttgaagga gcaaagggtg ttaaccagag
    11641 ttccccattt cccacgggtt acgcccatag agaacaggaa cataatttgg aagtcagtgg
    11701 tgcgggtagg tacaatgccg tggcgaccaa gccaggcagt gaccagcgcc gccggaacac
    11761 cggtttcttc cagttcacca tcttcaccca ttcccggagc aaggatgctg actttaatcg
    11821 ggtcgagcat actccagtta tccggaatat ctttgaagcc gtgccagctt tcgcccggat
    11881 gcattaccca gcagtcctga acggtggtca gcagtttggt tggtgcgtca gcaaagtcat
    11941 aggttttgcc ggtttgtggg tcggtgacga cttctttgtt ccacggtttg aagaaccagc
    12001 taccgtcagc ggtgaactct ttatacagcc gcgccatcgc ctgacgaaaa tcaaccgctt
    12061 cgtcaatcac ttcttgtgtc agtgacaggc cgctgttgcc gtccatcatc gacaccgcca
    12121 cgtcgttgga tgcgcagatg gcatacagcg gggaggtggt ggcatgcatc atgtaggcct
    12181 ggttgaagcg ggagaagtta atcgccccac gaccttcacg tacatgaata taagaagcct
    12241 gtgacagcgc attcagcagt ttgtgggtgg agtgggtggc gaaaacggta ggaccgttgt
    12301 gatcgccagg ttcgccgcgc atggcatagt gatcggcata gatcgggttg aaacgtgcat
    12361 agccgtacca ggcttcgtca aagtgcagac gatcggaggt tttttccagc agatcctgcg
    12421 cttctttagc gttataacac acgccgtcat aggtgcagtt ggtcaccacg cagtaagacg
    12481 gtttttgccc agctttgtct ttagtcagcg ggctttcact gattttcttc tgcaaggttt
    12541 caggttgcat ttcctgcgga tagattggcc cgataatgcc gtagcggttg cggcttggca
    12601 ccatgtagac tggtttcgcg cctgtcagca tcaagccttg ttcgatggat ttatggcaat
    12661 tacggtcaac gaccacgaca tcgttatcgg tcatgcaagc ctgcatgatg gtgcggttag
    12721 agccggaagt accgacgact accgaccagg agcgatcggc accaaatacg cgtgcggcat
    12781 atttttcgct ttcgccaaat gcgccagtat ggtcaagcaa agaaccgagg gaagttcgtt
    12841 cgatgcccat gtcggtgcga aacagatttt caccatagta gtcatggtag aaacgtccgg
    12901 cgggtgtttt ggtaaaacca acgccgccct ggtggcctgg cgctgcccag gaatattcat
    12961 ggatgtcact atatttcatc agcgcgctga acagtggcgg caacagctgc tggcggtagc
    13021 gggtcatcgc ggcaacggcg cgtccggcga taaagtcggc ggtatcttcc agaatccagg
    13081 cgaattcatc gacaagctcc agcagatccc ggtccattgc ggcgagggct ttttcccgat
    13141 cgcccaacag gaagaccggc acgttttgtt ggcgctcatg aagcttaccg atcaattgtc
    13201 tgacgttttg atgttcgtcc ggatgttcca tttgatagct gaacatcagg cagtcaatgg
    13261 cttcgtttga agagagaatg gcaaaaccat catcaaagga ggtggattta atcacggtaa
    13321 cattttgctg gcttaaagca tctgccagac gctcaacggc gttaccgacc caggtgtctt
    13381 gatggagaaa ctcgctttca acaattaata ctttcatcat tgcttacccg gttatgaagg
    13441 aatcttcgtt gcgggcaagt atcttccggc cttttcgggc tgtaaagcgc gtgaaaaaca
    13501 aacatttttg aaatatttag ttttataaac aatacgttat gtgattattt taaattattt
    13561 tcactgctta ttatcgacgg ctaaactatt tttttggctg atactgatat cgtctttagc
    13621 cgggaaacgt ctggcggcgc tgttggctaa gtttgcggta ttgttgcggc gacatgccga
    13681 catatttgcc gaacgtgctg taaaaacgac tacttgaacg aaagcctgcc gtcagggcaa
    13741 tatcgagaat acttttatcg gtatcgctca gtaacgcgcg aacgtggttg atgcgcatcg
    13801 cggtaatgta ctgtttcatc gtcaattgca tgacccgctg gaatatcccc attgcatagt
    13861 tggcgttaag tttgacgtgc tcagccacat cgttgatggt cagcgcctga tcatagtttt
    13921 cggcaataaa gcccagcatc tggctaacat aaaattgcgc atggcgcgag acgctgtttt
    13981 tgtgtgtgcg cgaggtttta ttgaccagaa tcggttccca gccagagagg ctaaatcgct
    14041 tgagcatcag gccaatttca tcaatggcga gctggcgaat ttgctcgttc ggactgttta
    14101 attcctgctg ccagcggcgc acttcaaacg ggctaagttg ctgtgtggcc agtgatttga
    14161 tcaccatgcc gtgagtgacg tggttaatca ggtctttatc cagcggccag gagagaaaca
    14221 gatgcatcgg cagattaaaa atcgccatgc tctgacaggt tccggtatct gttagttggt
    14281 gcggtgtaca ggcccagaac agcgtgatat gaccctgatt gatattcact ttttcattgt
    14341 tgatcaggta ttccacatcg ccatcgaaag gcacattcac ttcgacctga ccatgccagt
    14401 ggctggtggg catgatatgc ggtgcgcgaa actcaatctc catccgctgg tattccgaat
    14461 acagcgacag cgggctgcgg gtctgttttt cgtcgctgct gcacataaac gtatctgtat
    14521 tcatggatgg ctctctttcc tggaatatca gaattatggc aggagtgagg gaggatgact
    14581 gcgagtggga gcacggtttt caccctcttc ccagagaggc gaggggagcg tccgagcatc
    14641 atgaggccga aaactctgct tttcaggtaa attattacca taaactcaga tttactgctg
    14701 cttcacgcag gatctgagtt tatgggaatg ctcaacttgg aagccggagg ttttctgcag
    14761 attcgcctgc catgatgaag ttattcaagc aagccaggag atctgcatga tgactgcacc
    14821 caaaattaca tttatcggcg ctggttcgac gattttcgtt aaaaatattc ttggtgatgt
    14881 gttccatcgc gaggcgctga aaacggcgca tattgccctg atggacattg atcccacccg
    14941 cctggaagag tcgcatattg tggtgcgtaa gctgatggat tcagcagggg ccagcggcaa
    15001 aatcacctgc cacacccaac agaaagaagc cttagaggat gccgattttg tcgtggtggc
    15061 atttcagatt ggcggttatg aaccttgcac ggtgactgat ttcgaggtct gtaagcggca
    15121 tggtctggaa caaaccattg ccgatacgtt ggggccgggc ggtattatgc gcgcgctacg
    15181 taccattccg catctgtggc aaatttgcga ggacatgacg gaagtctgcc ccgatgccac
    15241 catgctcaac tatgttaacc ccatggcgat gaatacctgg gcgatgtatg cccgttatcc
    15301 gcatatcaaa caggtcgggc tgtgccattc ggtgcaggga acggcggaag agctggcgcg
    15361 tgatctcaat atcgacccag ctacgctgcg ttaccgctgc gcaggtatca accatatggc
    15421 gttttacctg gagctggagc gcaaaaccgc cgacggcagt tacgtgaatc tctacccgga
    15481 actgctggcg gcttatgaag cagggcaggc accgaagcca aatattcatg gtaatactcg
    15541 ctgccagaat attgtgcgct atgaaatgtt caaaaagctg ggctacttcg tcacggaatc
    15601 gtcagaacat tttgccgaat atacgccgtg gtttattaag ccaggtcgtg aggatttgat
    15661 tgagcgttat aaagtaccgc tggatgagta cccgaaacgc tgcgtcgagc agctggcgaa
    15721 ctggcataaa gagctggagg agtataaaaa cgcctcccgg attgatatta aaccgtcacg
    15781 ggaatatgcc agcacaatca tgaacgctat ctggactggc gagccgagtg tgatttacgg
    15841 caacgtccgt aacgatggtt tgattgataa cctgccacaa ggatgttgcg tggaagtagc
    15901 ctgtctggtt gatgctaatg gcattcagcc aaccaaagtc ggtacgctac cttcgcatct
    15961 ggccgccctg atgcaaacca acatcaacgt acagacgctg ctgaccgaag ctattcttac
    16021 ggaaaatcgc gaccgtgttt accacgccgc gatgatggac ccgcatactg ccgccgtgct
    16081 gggcattgac gaaatatatg ctcttgttga cgacctgatt gccgcccacg gcgactggct
    16141 gccaggctgg ttgcaccgtt aaaacgcgac taaacgctac tgcgccgggg gatttattcc
    16201 ggcgcacacc tctgacgata ccaataacag aaggcgggcg ttggtaacag cgacccgata
    16261 ccctatgagc atttcaatga ctacaaaact cagttatgga tttggagcgt tcgggaagga
    16321 ttttgcgatc ggcattgtgt atatgtacct catgtattac tacaccgatg tcgtcgggct
    16381 gtctgtgggt ttggtcggta ctttgtttct ggtggcgagg atctgggatg ctattaacga
    16441 tccgattatg ggatggattg taaatgctac gcgatcgcga tggggtaagt tcaaaccctg
    16501 gatcctgatc ggtacgttgg caaactctgt aatcttattt ctcctcttta gtgcgcatct
    16561 gtttgaaggt actactcaga ttgtctttgt ttgcgtgacc tacatcctct ggggcatgac
    16621 ttacaccatt atggatattc ccttctggtc gctggttcca accatcacgc tcgataaacg
    16681 tgagcgcgaa caactggttc cttatccgcg tttttttgcc agtctggcag gctttgttac
    16741 ggcaggtgtg acgctaccat ttgttaatta tgtcggcggt ggcgatcggg gatttggctt
    16801 tcagatgttc actctggtac tgatcgcctt ttttattgtt tcaaccatca tcactctgcg
    16861 caatgtgcat gaagtctttt cgtcagacaa tcaaccgtct gctgaaggaa gccatctgac
    16921 acttaaagcc atcgttgcgc taatttataa aaacgatcag ctttcatgcc tcttgggtat
    16981 ggctcttgct tataatgtag ccagcaacat tattaccgga tttgctatct attatttctc
    17041 atatgttatc ggtgatgcgg atttgttccc ctattatctg tcgtatgcgg gagctgctaa
    17101 cctggtgacg ttagtattct tcccacgctt agttaaatca ttatcccgac gcattttatg
    17161 ggccggagca tctattcttc cggtgttaag ctgtggtgtt ctcctgttaa tggcattaat
    17221 gagctatcac aacgtcgtcc tcattgtgat tgcgggtatt ttgctgaatg tgggaacggc
    17281 gcttttctgg gtattacagg tcatcatggt ggcagatacc gttgattacg gtgaatataa
    17341 actgcacgta cgctgtgaaa gtatcgctta ctccgtgcag actatggtgg tgaagggcgg
    17401 ttcagccttt gcggcttttt tcattgcggt tgtgttaggg atgattggct atgtaccgaa
    17461 tgttgaacag tctacgcaag ccctattagg tatgcagttt attatgattg ctctaccaac
    17521 tctgtttttc atggtaacgc tgattctcta cttccgtttc tatcgcctca atggcgacac
    17581 gctgcgcagg atccagattc atctgctgga taaatatcgc aaagtaccgc ccgagcctgt
    17641 tcatgctgat attccggtcg gtgcagtgag tgatgtgaaa gcctgacgtg gacaatagct
    17701 aacgaaaatg gcctgatgcg atatgtttat caggccacgt cttaatgtaa tgctttggat
    17761 ttgcaaattt tgtagaccgg ataaggaatt cacgccgcat ccggcatcaa caaagcgcac
    17821 gttgttatcc ggttatcaag ccaaagcgcc gtagctggcg gcaatgaaag cgagcgagga
    17881 atatcaccgt cgttaatgcg ccaagcagcg cgcagaacat atcggattgc gtgtcccact
    17941 ggtcgccctg ggtgccgaga aaatcatccg ctccctggcc cattgccagc gccgcccacc
    18001 actcgattaa ttcatacatc gcgcttatcg ccagcgccac gcagcagacc aggaacgcca
    18061 ccattttacg tccgcgcacg tacatcccgc gaacgagaat ttctcgtgcc accagtgcag
    18121 gcaccagccc ctggaaaaaa tgccccagct tgtcatacgg attacggctc aaccctaacc
    18181 attcctgcac ctcaaaacca acggggactt tcgcgtaggt gtattgtccg ccgaccatca
    18241 ggatgatggc gtgaaagaaa atgagcgtat agagcagcgg cgttaacgga taacgtttcg
    18301 cggtggcaag gagtagcggc acgacaataa tcaccggtgt cacttccatc agccaggtga
    18361 gtttgtcatg ggccgaaatg ccggtataaa tcaggattaa cgttagcgcc agtgcgccgg
    18421 tgttaagaat taacggcttg agtgtgcggg tcatggtgag ttcgcaagtc agggaaaata
    18481 ctgactattc accagcaagc gtgaaattac aatcgcaaaa gaggtggccc agggggatca
    18541 cctggcagca tgctgccagg cgctgggccg aagaggttac ttagtgcagt tcgcgcactg
    18601 tttgttgacg atctgctgga agaagtcgtt acctttgtca tcgaccagga taaacgccgg
    18661 gaaatcttct acttcgattt tccagatagc ttccataccc agctccggat aagcgacgca
    18721 ttccagatgc ttgatgctct gctgcgccag taccgccgcc ggaccgccga tgctaccgag
    18781 gtagaagccg ccgtgtttat gacacgcgtc ggtaacctgc tgactgcggt tacctttcgc
    18841 cagcatgatc atgctgccgc cgtgggattg cagcagatcc acgtaggagt ccatacggcc
    18901 tgcggtggtt gggccaagtg aacctgatgg ataaccggca ggggttttcg ccggacccgc
    18961 gtagtagatc gggtgatctt tgatgtactg cggaagttct ttaccggcgt caatcagctc
    19021 tttcagcttg gcgtgtgcaa tatctcggcc cacgataatg gtgccggtga gcgacaaacg
    19081 agtggatacc gggtattgcg aaagctgggc gaggatctct ttcatcgggc ggttaaggtc
    19141 aactttcacc gcttcgcctt caccggcctg gcgcagttct tgtggaatgt actggcctgg
    19201 gttgtgttcc agtttttcga tccagatacc tttgcggttg attttcgctt taatgttacg
    19261 gtcagcggag caggagacgc ccatgccgac cgggcaggat gcgccgtgac gtggcagacg
    19321 gataacgcga atgtcgtgcg cgaagtattt accgccaaac tgcgcgccaa gaccgagttt
    19381 ctgggcctct tccagcagtt cctgttccag ctggacatcg cggaacgcct gaccatgttc
    19441 gttcccttcc gtcggcagtt catcgtaata gtgagcgctt gctaacttga cggttttcag
    19501 gttggtttcc gcagacgtac cgccaatcac aaacgcgata tggtacggcg ggcaggctgc
    19561 agtaccgagg gtacgcattt tctcgacgag gaagtttttc agtttgccgg gagtcagcag
    19621 ggctttggtt tcctggtaga gatacgtttt gttggcagag ccgccgcctt tcgcaacgca
    19681 aaggaatttg tactcatcgc catctaccgc gtacaggtcg atttgcgcag gcaggttagt
    19741 gccggtgttg acctctttgt acatgtccag cgccgcattc tgtgaatagc gcaggttatc
    19801 ttcgatatag gtgttataga cgcctttcga cagcgcttct tcatcaccgc cgccggtcca
    19861 cacgcgctgg ccttttttac cgacgatgat cgcggtgccg gtatcctggc aggtcggcag
    19921 cacgcctttg gcggcgattt cggagtttct taagaattgc agcgccacgt acttgtcgtt
    19981 ttcgctggct tctggatcgt gaagaatagc cgcaacctgt ttctggtgtg ccgggcggag
    20041 cataaaagaa gcgtcgtgaa aggcttgctg cgccagcagg gtcagggctt ctggttccac
    20101 tttcaggatg gtttcgccgt cgaagtcggc aacgctaacg taatcggaag tgagtagata
    20161 gtattcggta ttgtctttcc ccatcgggaa aggtgcctgg tagataaagg gtttgtttga
    20221 catagcttcc agcctgtaac tctgtatttg ttattcgaaa atggcgtgcc gcgtagtcac
    20281 gggcggcacg caaagtgcat ttataagaac ccgtacatcg cggcgaagat ccagccgaag
    20341 acgcacgata cgctcacacc aatcaacccc ggcagaataa agctgtggtt gatgacgaag
    20401 cgaccgatgt gggtggtgcc ggaacggtca aactgaatcg ctgccagatc gctcggataa
    20461 gtcggcagga tgtaataacc gtagcaagcc ggtgctgaag ccacgatgta tgccggatca
    20521 acgccgatcg ccagcgcgac cggaacaatc gccgccagcg ccgcagcctg agagtttaca
    20581 aacttggaaa ccagcagcag aacaatggca taggcccacg gatactcttt caccatttca
    20641 cccagtacgc cctgaatttc agacatatgc gcaccgaaca tggtttctgc catccatgcg
    20701 ataccgtaca ccgccacgat ggcgatcata ccggaacgga agacttcgtt ttttgagata
    20761 gacgcgggat tggttttggt caggataata atcagcgccc cggtcagcag cataaacatc
    20821 tgaataacca gtaccatcga cagcggtttg ccgccgaagg atggacgcag gtccgaatca
    20881 gcaccaagaa gggcgactac agcgattgca ccgaggaaaa tccacattgc cagccagttg
    20941 cttttcggca gttttttatc gagcagcgtc gcggtatcac cgtaaacata ctcacggttt
    21001 tccggtacgg agatgaattt ctggaactct tcgtctttat ccagatcttt accgcggaac
    21061 cagctgaaga taccgatcgc caggataccg attaacgtcg atggaatggt gattgccagc
    21121 aggtcgagga actcaagatg gcgaccatca aaggtgacat tacccagcat tgcaaccaga
    21181 gacacgaccg caaccgacac cggactggcg ataatcccca tctgtgcacc gatagaactt
    21241 gccgccatcg gacgttccgg acggatgttg ttcttaatgg cgacgtcgta gatgatcggc
    21301 agaatggtgt aaaccacatg acccgtaccg caaagaatgg tcagtgtaca ggtcacaaac
    21361 ggcgcgacaa ttgagacata tttcgggttg cggcgcagca acttctcggc aatttgcagc
    21421 atgacatcaa gaccgcccga agcttgcaag gtcgccgatg ccgccaccac cgcaatgata
    21481 accagcatga catcaactgg tggtttacct ggctgaaggt ggaagacgaa gaccagaatg
    21541 accagaccga taccgcctaa taaacccagc gcgataccac cctttctggc accataaaac
    21601 agacatatca gtattatgat aagttggata gtaaataaca tgtgtgaacc ctcgcgataa
    21661 tcctatttaa atttttgctg aatagatcac agtcacgttc tgttttgtat gaactgtttt
    21721 cagagcctga ctaaatatcc gtttggtcgt taccggcttt agcaaatgcc tcacagtgaa
    21781 tattggctga ttaaagcagc ggcaaattta tgcactgtga taagcggctt tttcagagag
    21841 aaaaactctc tggcgatggg tttatagttt gtactgcgca gatgttaatg ttttgtttgc
    21901 tgttatctta ttgatattta tcggtctatt ttttgttctc agggcagata tcattaggtt
    21961 taaagattaa tctgatctac ccatttgtgg gtaaaaatac acataatgcg ggtgacataa
    22021 tagttaatta acttttgtta gcgttttgaa attaaaaaca ccgttcacct gaagagatat
    22081 taatttttag cgatgatgga gggataatta tatttgatct ggcacaagtt ttactgatga
    22141 aggatgtaac ttgtgccagg ggtattttgc attacggtaa taattattac ttacagataa
    22201 ccagcgatcc gttattggca atattgtttc agtagtgagt agtgttctgc ctgaatacgg
    22261 taacggtaaa ctggacgccc cgtgacgcca taatggatac tggtgaacaa gatgtggcag
    22321 ttgaccagcc agatgaggta tttacggcag gaaacacgcg aaatgttaac ctcgttggct
    22381 agctcgtcgg ttgaaaactc atagtcctga tgcgcgtcaa tccactggca cagtgtgcgt
    22441 aacgtctgcg gcgttaagcc ttttggcaag cgacgaggat cctgttcgtt ggagctgctg
    22501 ccgtggatta gctgatcaag ctcggcctgg tcataatact gatgtttttc cagcgccatt
    22561 ttcttttgcc gccagccggt gagcgcctct tcaaagcggg aagcctggaa gggtttgatc
    22621 aggtaatcca cgacaccgta atgcagcgaa tctttaatgg ttgccgcatc ggctgcggag
    22681 gagatgacaa tcacatcact tttgcaacgc gcgttatgca ggacaggcag taaatcgagc
    22741 ccgttctctt tttgcatata gatatcgagc aatatcaggt cgataggcgt atcgctattg
    22801 aagatcatct ctttggcttt ctccagcgtc gaggctgttc cacagcattg aaagcctggg
    22861 atttgtgcta cgtatcggcg attcagctcc gcgaccattg cgtcgtcatc gataattaat
    22921 acattgatca tctgttcgac ctctccccgt cccagggtat ctggacaaaa aattgtgtga
    22981 aaatcccggg ttccgattcc acggcgatgc tgccgccgag attttctacc tgttgtttga
    23041 caagtgctaa accgacgcct cgctcgcttc cttttgtcga gacacctttg tcaaaaatgt
    23101 gatcgatttt gtcgggtgcg atccccggtc catcatcgtt aacttcgcag tgcagccagc
    23161 cgtgacggta gtgcaatgtt acgctaattt cgccaccggg ttccggccct aatgcttcca
    23221 gcgcgttttc tatcagattt cccaatgtgg taatcagcgt cgcgacctgg tcctcactgc
    23281 cgctgtctgg cagctggctt tcactgttta aaatcagcgt atggcctaaa tcggtcgcgc
    23341 gattaatctt gctgattaaa aaaccagcga taaccggaga tttgatctta cccagcagag
    23401 agccaatctc ttcctgatag ttattggctg ttttgagaat gtaatcttcc aactgcttat
    23461 aactcttcag atgcaataat ccgagaatca catgcaattt attcataaat tcgtgggatc
    23521 gttcacgaag tgcatcagca tagttgacca gaccgtcgag tcgctgcatc agtttacgaa
    23581 cttcagtttt gtccctgaag gttgaaatgg caccgatgat aacgccatta ctgcgcaccg
    23641 gaacggtgtt gatcagtaat agccggtctt taatcgtaat ctcttcgtcg cggcgcggcg
    23701 taccgtcgcg taacacttcc gagacatcta ccacctgtga ccatgagtgg cttagcgtcg
    23761 acagtttctc atcgtcctgc gacttacggt aattcagcaa ttcttgtgcg gcatcgttga
    23821 tcagcgtgac ctcgccgcga tcgtccacgg caacgacgcc ttctttgata gactgcaaca
    23881 tggcctggcg ctgctcaaac agcgtggaga tttcgtaggg ttccaggccg aaaaggattt
    23941 ttttcagtac cttaaccaga atgcaggtgc caatcagtcc gaccagcatg ccaaataata
    24001 ccgaccagat aatgctccag cgactgtcat tgatttgttg ggtcacgcgg cttaactcaa
    24061 ggccgatcgc caccacgcca atttgtttat gattttcatc gtagatgggg gtaaatacgc
    24121 gtaaagcctg cgccagaaaa ccgcgattga tagcgacatt ttctttgcca ttcagcgctt
    24181 taaggatgtc atcaccttta aatggctgac caatacgctg ggcttcagga tgcgagtagc
    24241 gaagactttg catatcggta acgacaataa acagcagatc gttgcgtttg cgcacggctt
    24301 ccgcgatggc ctggatgccg ctctcctgcg gttttttctg caagccctga cggatttccg
    24361 gcgagtcggc gagggtacgc gccactgcca gtgccttgta ggctagcccg tctcgcgtca
    24421 tatcactgat ttgcgagaag taaatcagat gcaccaccaa tagcaccgag aacagtaccg
    24481 cactgaccat taagatcact gtggtactca atttcatcgg acgtttgcgt aacatgcggt
    24541 agggcaatga atgtctcatc agcttccttg tgtgacaaat ttcttaagca ttatctctga
    24601 tgaggcgggt aattcaaagg gagtaagaat gattggctat ataggggaag agattctggc
    24661 aacggaaact gccagtgctg tatgaagatt acagggctat gcttatagcg ataatcatac
    24721 tgatgagaga gggaaagtca tggatcaggc gctactggac gagggttatc gctgttatac
    24781 cggcgaaaag atcgatgtct atttcaacac tgcgatatgt cagcattctg gcaattgcgt
    24841 acgtggcaac ggcaagttat ttaatctcaa acgaaagccg tggatcatgc cggatgaagt
    24901 cgacgtcgcc actgtggtta aagtgattga tacgtgcccg agcggtgcgc tgaaataccg
    24961 tcataaataa gcgagggtaa aatggaaata cgcgaaggcc acaataagtt ttacattaat
    25021 gacgaacaag gcaagcaaat cgctgaaatt gtctttgtgc cgaccggaga gaatttagcg
    25081 attatcgaac ataccgatgt cgatgaaagc ctgaaagggc aggggattgg taaacagctg
    25141 gttgcaaaag tcgtggaaaa aatgcgtcgg gaaaaacgaa aaattatccc attatgtcca
    25201 tttgcgaaac acgaatttga taaaacgcgg gagtatgatg atattcgcag ttgatgggag
    25261 agtacagatt cacgatattt ttcatgctct ccgcggtgtg atgcaggaga gcatctgaag
    25321 ggtaggggga tgcacaaaga atgggcagag agagcgtttt tttgtcccaa gtcatcccct
    25381 ttactgagca aaaaaaagaa tatctcctat atgagaatca tcaatcgggg ttaataagtt
    25441 ttgcgtcccc agagcgttca atattgatag gagtcatatt atggaaggta aaaacaagtt
    25501 caatacttat gttgtttctt ttgattatcc atcatcttat tcctcagtgt tcttaaggtt
    25561 aagatcattg atgtatgata tgaatttctc ttctatcgtg gctgatgaat atgggatacc
    25621 acgacaattg aatgaaaatt ccttcgcaat aacgacatca ttagccgcaa gtgaaatcga
    25681 agatttaatc aggctcaaat gcttagactt accggatatt gattttgacc tcaacattat
    25741 gacagttgat gactatttcc gtcagtttta caagtagcca ggaaggataa gaagaacata
    25801 aaaaatggca ctattctcta aaatat
//
LOCUS       DAZGVQ010000067        25042 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000067 DAZGVQ010000000
VERSION     DAZGVQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.067"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1080
                     /locus_tag="ACHLAG_002607"
     CDS             <1..1080
                     /locus_tag="ACHLAG_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFP0655813.1"
                     /translation="IYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLSFICLTSL
                     ALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVDYCPDNHT
                     ATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLLTLDEYNY
                     TIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFFNSVESLL
                     SRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDILARLEGEV
                     FGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTGDTRNISL
                     VITEADKALREAKSEGGNKVIIHHI"
     gene            1261..2751
                     /gene="dgcJ"
                     /locus_tag="ACHLAG_002608"
     CDS             1261..2751
                     /gene="dgcJ"
                     /locus_tag="ACHLAG_002608"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFP0655814.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHE
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2794..3297
                     /gene="yeaK"
                     /locus_tag="ACHLAG_002609"
     CDS             2794..3297
                     /gene="yeaK"
                     /locus_tag="ACHLAG_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFP0655815.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHSKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3298..3402)
                     /gene="yoaI"
                     /locus_tag="ACHLAG_002610"
     CDS             complement(3298..3402)
                     /gene="yoaI"
                     /locus_tag="ACHLAG_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFP0655816.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3572..4018
                     /gene="yeaL"
                     /locus_tag="ACHLAG_002611"
     CDS             3572..4018
                     /gene="yeaL"
                     /locus_tag="ACHLAG_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFP0655817.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(3975..4796)
                     /gene="nimR"
                     /locus_tag="ACHLAG_002612"
     CDS             complement(3975..4796)
                     /gene="nimR"
                     /locus_tag="ACHLAG_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFP0655818.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMSTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKQDPRQ"
     gene            4893..6074
                     /gene="nimT"
                     /locus_tag="ACHLAG_002613"
     CDS             4893..6074
                     /gene="nimT"
                     /locus_tag="ACHLAG_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFP0655819.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6129..6476
                     /gene="yeaO"
                     /locus_tag="ACHLAG_002614"
     CDS             6129..6476
                     /gene="yeaO"
                     /locus_tag="ACHLAG_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFP0655820.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6498..6752)
                     /gene="yoaF"
                     /locus_tag="ACHLAG_002615"
     CDS             complement(6498..6752)
                     /gene="yoaF"
                     /locus_tag="ACHLAG_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFP0655821.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            6935..7960
                     /gene="dgcP"
                     /locus_tag="ACHLAG_002616"
     CDS             6935..7960
                     /gene="dgcP"
                     /locus_tag="ACHLAG_002616"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFP0655822.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            7993..8091
                     /gene="yoaK"
                     /locus_tag="ACHLAG_002617"
     CDS             7993..8091
                     /gene="yoaK"
                     /locus_tag="ACHLAG_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFP0655823.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8094..8168
                     /gene="yoaJ"
                     /locus_tag="ACHLAG_002618"
     CDS             8094..8168
                     /gene="yoaJ"
                     /locus_tag="ACHLAG_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFP0655824.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8227..8475)
                     /gene="yeaQ"
                     /locus_tag="ACHLAG_002619"
     CDS             complement(8227..8475)
                     /gene="yeaQ"
                     /locus_tag="ACHLAG_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP0655825.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8702..9910)
                     /locus_tag="ACHLAG_002620"
     CDS             complement(8702..9910)
                     /locus_tag="ACHLAG_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP0655826.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFGPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSQFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            9918..10343
                     /gene="tnpA"
                     /locus_tag="ACHLAG_002621"
                     /pseudo
     CDS             9918..10343
                     /gene="tnpA"
                     /locus_tag="ACHLAG_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(10365..10553)
                     /locus_tag="ACHLAG_002622"
     CDS             complement(10365..10553)
                     /locus_tag="ACHLAG_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFP0655827.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(10557..10916)
                     /gene="yeaR"
                     /locus_tag="ACHLAG_002623"
     CDS             complement(10557..10916)
                     /gene="yeaR"
                     /locus_tag="ACHLAG_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFP0655828.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(11089..11727)
                     /gene="leuE"
                     /locus_tag="ACHLAG_002624"
     CDS             complement(11089..11727)
                     /gene="leuE"
                     /locus_tag="ACHLAG_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFP0655829.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(11854..12777)
                     /gene="dmlR"
                     /locus_tag="ACHLAG_002625"
     CDS             complement(11854..12777)
                     /gene="dmlR"
                     /locus_tag="ACHLAG_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFP0655830.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            12880..13965
                     /gene="dmlA"
                     /locus_tag="ACHLAG_002626"
     CDS             12880..13965
                     /gene="dmlA"
                     /locus_tag="ACHLAG_002626"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFP0655831.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            14216..15826
                     /gene="yeaV"
                     /locus_tag="ACHLAG_002627"
     CDS             14216..15826
                     /gene="yeaV"
                     /locus_tag="ACHLAG_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFP0655832.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            15858..16982
                     /gene="yeaW"
                     /locus_tag="ACHLAG_002628"
     CDS             15858..16982
                     /gene="yeaW"
                     /locus_tag="ACHLAG_002628"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFP0655833.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            17038..18003
                     /gene="yeaX"
                     /locus_tag="ACHLAG_002629"
     CDS             17038..18003
                     /gene="yeaX"
                     /locus_tag="ACHLAG_002629"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFP0655834.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(18057..19172)
                     /gene="rnd"
                     /locus_tag="ACHLAG_002630"
     CDS             complement(18057..19172)
                     /gene="rnd"
                     /locus_tag="ACHLAG_002630"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFP0655835.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(19254..20939)
                     /gene="fadD"
                     /locus_tag="ACHLAG_002631"
     CDS             complement(19254..20939)
                     /gene="fadD"
                     /locus_tag="ACHLAG_002631"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFP0655836.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(21144..21725)
                     /gene="yeaY"
                     /locus_tag="ACHLAG_002632"
     CDS             complement(21144..21725)
                     /gene="yeaY"
                     /locus_tag="ACHLAG_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFP0655837.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADMN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(21765..22460)
                     /gene="tsaB"
                     /locus_tag="ACHLAG_002633"
     CDS             complement(21765..22460)
                     /gene="tsaB"
                     /locus_tag="ACHLAG_002633"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFP0655838.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPEIVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(22518..24428)
                     /gene="yoaA"
                     /locus_tag="ACHLAG_002634"
     CDS             complement(22518..24428)
                     /gene="yoaA"
                     /locus_tag="ACHLAG_002634"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFP0655839.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            24560..24904
                     /gene="yoaB"
                     /locus_tag="ACHLAG_002635"
     CDS             24560..24904
                     /gene="yoaB"
                     /locus_tag="ACHLAG_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP0655840.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         6085 a         6408 c         6076 g         6473 t
ORIGIN      
        1 atctatttag ttgaaaccat tgtaattatc cataaaccaa ttaacggcag tacactgatc
       61 cagacaaagt cgaatgatgt ttctattttc tatattttcc gccaactcag ttttatttgt
      121 ttaacctctc tggcactctt ttgttatgga aaagacaaca tccttgacaa caataagaaa
      181 aaaacgggaa tcctgttgct ggcgctgatt ccttttttag tttttcccct tctggcacac
      241 aatctgagca gttataacgc tgactattct ttgtatgtcg tcgactactg tccggacaac
      301 catactgcga cctggggaat caactataca aaaatattgg tttgtctgtg ggcattttta
      361 ctgttcttta ttatcatgcg tacacgatta gccagcgaac tatggccatt aatagcatta
      421 ttatgtctgg catcgctatg ctgcaactta cttctactga ctctggatga gtataattac
      481 accatctggt atatcagtcg cgggattgaa gtttccagta aactgtttgt tgtgtctttt
      541 ctgatttata acatttttca ggagctgcaa ctctccagca aactggcagt tcatgatgtg
      601 ctgaccaata tttataatcg gcgctacttt ttcaacagcg tagagtcatt attgtcgcga
      661 cctgttgtta aggacttctg tgtcatgctg gttgatatta atcagttcaa acgcatcaat
      721 gcccaatggg gacatcgtgt gggtgataaa gtgctggttt caattgtcga tattatccag
      781 caaagcatcc gccccgatga tattttagcg cgtctggagg gtgaggtgtt tggcttgcta
      841 tttaccgaac tcaatagtgc ccaggcaaaa atcattgcgg aacgtatgcg taaaaatgtc
      901 gaactcctga ccggctttag taacagatat gatgttcctg aacaaatgac catcagtatt
      961 ggcacggttt tttcaacggg tgacacgcgt aatatctcgc ttgtcatcac ggaagcagat
     1021 aaagccttac gcgaagcgaa aagcgagggg ggcaacaaag tgattattca tcatatttaa
     1081 gtgcaaaaat attcagagcc atgctttttg cgtggctttt gcatacaatt tattactatt
     1141 acccctaaat ttcccctccc cagcggcgca gagatgagta taattagcgc ccttgtgcca
     1201 ggccgcaatc gaactttatc tggttttctc gtttcactaa ccgaaggagt gccatttatc
     1261 atgaaattgc accatagaat gctccggcat tttatcgccg caagtgtcat tgtgctgaca
     1321 tcttccttcc ttatttttga acttgtcgcc agcgacagag caatgagtgc ctatctgcgc
     1381 tatatcgtgc agaaagcaga ctcctccttt ctttatgata agtatcagaa tcagagtatt
     1441 gccgcgcatg tgatgcgcgc tctcgctgct gagcagtcgg aagtgtcgcc agaacagcgg
     1501 cgcgccatct gcgaggcttt tgagtctgcc aataataccc atggcttaaa cctgactgcc
     1561 catgaatacc cgggcttacg cggcacacta caaaccgcat ccactgactg cgacacaatt
     1621 gtggaagctg cagcactatt acccgctttt gatcaggcag tggaaggcaa ccgccaccag
     1681 gatgattacg gttcaggtct tgggatggcc gaagagaaat ttcactatta tctcgatctc
     1741 aatgaccgct atgtctattt ttatgagccg gttaatgttg aatactttgc gatgaataac
     1801 tggtccttcc tgcagtcagg aagtattggc atcgatcgca aagatattga aaaggtattt
     1861 accgggcgta ccgtattgtc gagcatttac caggatcagc gtactaaaca gaacgtgatg
     1921 agtttgctga cgccggtata tgtcgcaggg cagctaaaag ggattgtgct gctggatatt
     1981 aacaaaaaca atttgcggaa tatcttttac actcatgacc gccctctcct ctggcgtttt
     2041 ctcaatgtca cgctaaccga taccgattcg gggcgcgaca ttatcatcaa ccagagcgaa
     2101 gataatctgt tccagtatgt cagttacgtc catgacttac cgggcggcat tcgtgtctcg
     2161 ttatccattg atattcttta ctttatcacg tcttcgtgga aaagcgttct gttctggatt
     2221 ttgacggcgt taattttgct gaatatggtg cggatgcact tccgcttata ccaaaatgtg
     2281 tcgcgagaaa atattagtga tgcgatgact ggactgtata atcgcaaaat tttaacccct
     2341 gaactggagc agcggttgca gaaactggtg caatccggtt cttcggtgat gtttattgca
     2401 attgacatgg acaagttaaa gctaataaat gacaccctcg gtcatcagga aggggattta
     2461 gcgattacgt tgttagctca ggcgattaaa caatcgattc gtaaaagtga ttatgccatc
     2521 cgactcggtg gcgatgaatt ctgcatcatt cttgtcgatt cgacgccgca aattgcagca
     2581 caactgcctg aacgtatcga aaaacgtctg caacatatcg cgccgcagaa agagatcggc
     2641 ttctcttccg gtatttacgc gatgaaagaa aacgatacgt tacatgatgc gtataaagct
     2701 tccgatgagc gtttatatgt caataagcag aacaaaaaca gccgttcatg ataaccttct
     2761 gtggttgttt gcttgtaatc tcaggagcgt gaaatgactg aaatggctaa aggaagcgtg
     2821 acgcatcagc ggttaatcgc tttattatca caagaaggtg ctaacttccg cgtcgttacc
     2881 catgaagcgg taggtaaatg tgaagcggtg tcagaaattc gtggcaccgc tttaggccag
     2941 ggagctaagg cactggtatg taaagtcaaa ggcaatggcg taaatcaaca tgttctggcg
     3001 atcctcgctg ccgatcaaca ggccgatctg agccaactcg ccagccatat aggcggatta
     3061 cgcgcttcgt tggccagccc ggcggaagtc gatgaactca caggctgtgt cttcggcgcg
     3121 atcccccctt tcagcttcca ttcaaaactc aaactggttg ccgaccctct actcttcgaa
     3181 cgatttgatg aaatcgcctt caatgcaggg atgctggata aatccgttat tttgaaaacc
     3241 acggattatt tgcgcattgc gcaaccagaa ctgctcaatt tccgccgcac tgcgtaacta
     3301 gcctgtccgt tcaataagca gaacggacaa aaccagcaca acagcaatgg caaaaaacga
     3361 tgacgtgata atcagtgtct cgacgaacat ttgatcgttc atagcatgcc cccgggagat
     3421 agctgttttc acgttattgt tagcgtgcac aaatggcagt ttgatgacag ttcgcctgat
     3481 tttttatgca aaaaacgtaa agattttcta cttccttcct gcagcaagcg taaagtaagc
     3541 aggcttatta ttttttggca aggaaaccac gatgtttgat gtcactttgc tgatcctgct
     3601 cgggttagct gcgctgggct ttatcagtca taacaccact gtcgccgttt caattctggt
     3661 gttgatcatt gtccgcgtca caccgttaag cacctttttc ccgtggattg aaaaacaggg
     3721 gctaagtatc gggataatca ttctgacgat tggcgttatg gcccccatcg ccagcgggac
     3781 gctaccgccc tcaaccttga ttcactcgtt tctgaactgg aaatcgctgg tggcaattgc
     3841 agtaggcgtt attgtctcct ggctgggtgg gcgcggcgtg accttaatgg gcagccagcc
     3901 gcaactggtc gccgggctgc tggtaggcac tgttttaggc gtggcgctgt ttcgcggcgt
     3961 accggtcggc ccgcttattg ccgcgggtct tgtttcgctg attgtgggga aacagtagtt
     4021 aatcccgcaa tatatcggcc tggcgtttgc cccaggcctt tcttaaacat agtaataaag
     4081 gccgttgttg aatcataccc cagcgtatga gcaactttct gtacagtatc gcctttcact
     4141 aatccctgaa gtgccataat cagctgtaac tgctgacgcc actggcgaaa actcaacccc
     4201 gtctctttta ctatcagtcg cgcaagattg cgttcactca tcgcaaaaaa gccagcccat
     4261 tgccctaatg acccccactc gacgggccct ttcgccatca tctccaccat cgtgcggatt
     4321 ttaggatgag aagaaacggg caggtgcagc tgttgttgtg gctgctgagg gagttcatca
     4381 aacaggacct gaattaatct gcgggtcatc ggctcagcac gctgagtcgt cgtcctgttt
     4441 gccagggtta aaatcaattc ccggcataag ggcgatattt tcaatgtgca acatgttgtc
     4501 gacattgtca ctgcgctggg ttcgataaac agaaaacaga gttcagcgtt agctgtcact
     4561 tgattgctat gttccacccc gcccggtatc caaaccgcat attgcggcgg caccatccac
     4621 aaagcatttt ccaccgtaca ggtaattgca ccatgtaatg ctagtatcag ctgtcctttg
     4681 cggtgctgat gtacaggact ggttaactca tccgttccgg cgtgaataca aaatgcgacc
     4741 gcggcttcat ggtgtcggtc gggttcatag ccattgagat tcaacctgtg catcattttg
     4801 tccgaattta gcgataattt gtcattttag cttgattcaa cataacaata aaaacggtaa
     4861 ggtacagcct cgtttgtaac aatgagaagc atatgacctg ttcaacttca ttaagcggca
     4921 aaaacaggat tgtccttatc gctggcattc tgatgattgc cacaacatta cgcgtcacct
     4981 ttaccggcgc agcgccgtta ctggatacga ttcgttccgc ttactcgctg acgacagcgc
     5041 aaaccggctt attaaccacc ctgccattat tggcctttgc gctaatctca cctttggctg
     5101 ccccggtggc gcgacgtttt ggtatggaac gtagcctgtt tgccgcgtta cttttgatct
     5161 gtgcaggtat cgcaattcgt tctctccctt cgccttactt attatttggc ggtacagcgg
     5221 tcattggcgg tgggattgca ttaggcaatg tcttactgcc aggattaatt aaacgcgatt
     5281 tccctcattc cgtcgccaga cttaccggcg catattccct gacaatggga gctgcagcgg
     5341 cactgggatc ggctatggtc gtgccgctgg ctttgaacgg ttttggctgg caaggcgcgt
     5401 tgttcatgct gatgtgtttt cctctgctgg ctcttttttt atggctgcca cagtggcgaa
     5461 gccaacaaca tgcaaatttg agtacctcgc gcgccttaca tactcggggt atctggcgtt
     5521 cgccgcttgc ctggcaggtc acattgtttc ttgggatcaa ctcactggtc tattacgtga
     5581 ttattggctg gcttccggcg atcctcatca gtcgcggcta tagcgaagca caggcgggtt
     5641 cactgcatgg tttgctgcaa ctagccacag cagctcccgg tttgctgatc cctcttttct
     5701 tacatcatgt gaaagatcag cgtggtattg cagcgttcgt tgccttgatg tgcgcagtgg
     5761 gcgctgttgg gctctgcttt atgccagcgc acgcgatcac ctggactctg cttttcggtt
     5821 ttggttccgg cgcaacaatg atactggggt tgacgttcat tggtcttcgg gcaagttctg
     5881 cgcatcaggc ggcggcactc tcggggatgg cacaatccgt cgggtatttg ctggcagcct
     5941 gtgggccgcc gctgatgggt aaaatacacg atgctaacgg tgactggtcc gtaccactga
     6001 tgggtgttgc catactttca ctactgatgg cgattttcgg actttgcgcc gggagagaca
     6061 aagaaattcg ctaatatccg gtgctatagt gacgtgacaa atcatgcgtg aaagggagaa
     6121 caaacacgat gaatattcag tgcaaacgcg tttatgatcc ggctgaacag agcgatggtt
     6181 atcgcgtact ggtcgaccgc ctctggccgc gcggtatcaa aaaaaccgat ttagcccttg
     6241 atgagtggga taaagaaatc acgccgtcaa cggaactgcg caaagccttt cacggcgaag
     6301 tcgtcgattt tgcaaccttt cgcgagcaat atcttgcaga actggcgcaa cacgagcaag
     6361 aaggaaagcg gctggcggac atcgccaaaa aacagccgct gaccctgctc tacgcagcaa
     6421 aaaacaccac gcagaaccat gcgctggtgc tggccgactg gctacgtagc ttgtgatttt
     6481 agtacagcat ccggcggtta tttttcacca gccggatggt cccgtcgcca caatgcccat
     6541 tcatcaatcg tttcaccgcc cggtaatttg caattgttgc tgaccccttg cgctgtctgc
     6601 actggaatga gcgtcccgcc cttctgctgg caatagaccg acgccggatt tgccatacca
     6661 atctgcggcg gtttaggtgc ttctggctga gaagaggttg aacaaccagc caggaccagc
     6721 aagcaaggca aagcaaaact gataattttc atttattgat ctcacacatt tatctacgct
     6781 tagagtatcg cggtatcgtt ttgttttgca gcactatttt tattacattc actcaaaaca
     6841 tattacgtct tgtttcatct ttgttgatga tgttttatca tgcctgcaaa gattaaataa
     6901 tcagcattta cccgccgtat cctggagttg tttcgtgtca gatcagatta tcgcccgcgt
     6961 ctcgcaatcc cttgccaaag aacagtcact ggaaagtctg gtccgacagc ttctggagat
     7021 gctggaaatg gtcactgata tggaatcaac ctacctgacc aaagtggatg tcgaagcgcg
     7081 cctgcagcat attatgtttg cccgtaacag ccagaaaatg cacatcccgg agaattttac
     7141 cgtctcgtgg gattactcgt tatgcaaacg cgctattgat gaaaactgct ttttcagcga
     7201 tgaagtccct gaccgttggg gcgactgtat tgcggcacgc aatcttggaa tcaccacatt
     7261 tctgagtaca ccaattcact taccggatgg atcattctat ggcacgcttt gcgccgccag
     7321 cagtgagaag cgccagtgga gtgaacgcgc ggaacaggtt ttacagttat tcgccggact
     7381 gattgcacaa tatattcaaa aagaggcgtt ggttgaacag ctgcgcgaag ccaatgccgc
     7441 gctgattgcg caatcgtata ccgactcgtt aaccgggcta ccgaatcggc gggcgatttt
     7501 tgaaaatctg acgacgctgt tttctctcgc ccggcatctt aaccataaga taatgatcgc
     7561 gtttatcgat ctggataact tcaaattaat caatgaccgt tttggtcata atagtggcga
     7621 tctgtttctc attcaggttg gcgagcgcct taatacgctc cagcaaaatg gcgaagttat
     7681 tggtcgtctc ggcggtgatg agtttttggt tgtttcacta aacaacgaga atgcggatat
     7741 ttcgtcgctg cgagaacgta ttcaacagca aatacgtgga gaatatcact taggtgatgt
     7801 tgatttgtat tatcccggtg ccagtcttgg catagtagaa gtcgatcctg aaacaaccga
     7861 tgcagacagt gccctgcatg ctgccgatat tgcgatgtat caggagaaaa aacacaaaca
     7921 gaaaacacct tttgtcgcgc atccagcgct acattcctga ggcgtattca catccttttg
     7981 attggtgata acatgcgaat cggtattatt tttccggttg taatctttat tacagcggtc
     8041 gtatttttag catggttttt tattggcggc tatgctgccc cgggagcata aagatgaaaa
     8101 aaacaacgat tattatgatg ggtgtggcga ttattgtcgt tctcggcact gagctgggat
     8161 ggtggtaacg tcacctctaa aaaatagcaa aggctgcctg tgtgcagcct ttgtgcaatt
     8221 taagcgttaa cttttaatct tcctgtagat aaatagcacg acaatcgcac caataacggc
     8281 aaccacgaag ctgccaaaat tgaagccatc gactttacca aagccaaaca gtgtgctgat
     8341 ccatccgccg actacggcac cgactatccc cagcagaata gtcataaaga atccaccacc
     8401 atctttacct ggcatgatcc acttcgccag aataccggca ataagcccaa aaataatcca
     8461 tgacagaatg cccattgttt cctcacttat ctgttttgca ttagcgggtt agtcgctgat
     8521 aaaaagcata gcacaacatc gggagggcaa gatttgtgac gagcatcacg gaggattttt
     8581 ttgcgatggc gcagaaattg cgccatcaac gatcggtgat aattaccaac cacaaacatc
     8641 atgttcgttt tccgtgtctt gacatcctcc acgccctgaa tgacgaggat ccctgctacg
     8701 ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt caacgagcgg cctgactgca
     8761 ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa aatgttacga gcgccgttta
     8821 catcggcgtt cgctgtatat ccacatacct ggcatctgaa ttgactttgt gacaggcgat
     8881 tttctttcgc tgtatgacca cagcacgcgc aacgctggct tgtgtacgct ggcggaacag
     8941 caagcacctg accgccactc cagagctgct tgtactcaag ctggcggcgc atttcatacc
     9001 agccctgatc cagtatcgaa cggtttaaac ctgattttgc ccggacattg cgccccggct
     9061 ggctgaccgt acccgctgct gactttgaca tgttgctgac cttcaaatcc tcaatgacta
     9121 tcattgcgtg gtttttgctg acggtcgttg tgactttatg aaggtagtcc ctgcggatat
     9181 ttgcgatacg ggaatgcagt cgctgtattt tgcgtttctg cttctgccag ttattgctga
     9241 atttgacctt gcggcttaac tgtcgctgaa gtctcgccag cgtcttctgg tttttctgaa
     9301 aactgtttac aggcccaaag actgtgccat ctgacagcgt ggcgagttta gccacgccag
     9361 catccagccc gatcattgat gctgaagggt gaaccggagt tgatacttca ctttctgtct
     9421 gaatactgat gtaccactta ccgcaggact ggctgaccgt gacatttttc acaacacccg
     9481 tgacctgacg gctattccgg tagcgcatcc agcccagttt cggcagaaaa atacggctgt
     9541 tttcctgatc gagctttacg ccctgcgggt agcggaatgc atcattctgt ccccgctttt
     9601 tgaatcgggg aaaagcagcc cgcttccgga agaagttttt gtaagcccgc tcaaggtctt
     9661 tcagtgactg ttgcaatggc tgtgagggcg aatctttaag ccattgcgtt tcagtggcgt
     9721 ttttccactc aaccagccag gaagccattt tcccgtaagg gatgtattta ttaccggcct
     9781 catgattctc attctgacgt gccagagcac gattgaaaac gaaacgacat gcgcctgcga
     9841 agcgcctcat ttcacactcc tgttgaccac ctggtcttaa ctggaattta aatgcctgta
     9901 atcgtttcac aggtcaaatg atactttggt ctgtgaaaaa agaaaccgat atccggcgtg
     9961 gaaggcattg tgttttcctg atgcatgttc acctggtctt tgtcaccaga taccgactcc
    10021 tgatttttga ccatgacgcg acagaaaaac tacgcactta cttttcaaat gtatgtgctg
    10081 attttgaagc tgaacttgtt gaaatggatg gcgaaccaga tcacgtccat ttgttaatta
    10141 actatcctcc caaactggcg atatccagtc tggtaaacag cctcaaaggc gtatcgggta
    10201 ggttactgcg acgagatcga ccagatattg cagtcaggta ttactacaaa ggcgttttgt
    10261 ggagtcctgg ctattttgcc agtagctgcg gaggtgcgcc aatatccgtc atccgccaat
    10321 acattgaaca acagcaaaca cctggtcagg tggaaaaccg cgccttatat ccccgccctg
    10381 aaggacgggg ttttacggcg caccggataa gaacgcacgg tattcaccag atcttttatc
    10441 acttcagccg ccacttctgg caccagcaaa gtcatcggcg tctctgtttc ataatcgaca
    10501 gaaacgccat tgctgttatt ggtgacggtc acggtatacg ttgctttgcc catgattcat
    10561 ttcccgttat gaatgacttt ccgttgttgc gcaccttcca tcaggacttc aggagccacg
    10621 aagaagtcaa tgttgaaata agtatcgtca gtcatggctt caatgttgtg ccacttttct
    10681 ggagggaaca ccgcaaactg ccccgcttcg ataaggatca cctgatcagg ctctgcactg
    10741 tgttcatcag cgtagccgag atatttgacc gccccatgca taacggaaag gcgtgggtaa
    10801 acccccgggc gcgttccttt atcaagatga cgttcgaata ttccggcagg cgcagtttgt
    10861 ttattccaga aaggcgttga gcgcgtatga atataattct gtgggatttg cagcatccta
    10921 ttccctcctt cggggaatgc gctgaaaacg gtttattcca gccgtttcag ggtacgcctg
    10981 ataatttgca ttttaaatac catttattgg ttacttttta gcaccatatc agagaaaaat
    11041 cagggaggat tacaagtggg aagcccgcga caacgcgggc tgaatgtatc aggattgcag
    11101 cgtcgccagt cgggcggcga aacccacgaa catcaaacca atcagtgagt tgccaacttt
    11161 agccagtttc tttttggtac gtatgtactg cgtgacaaaa gcaccagaaa taatcaggaa
    11221 gctcaaatag cagaaactca ccagttccag cgtcgtcgcc agaataaaga atgaaattcc
    11281 cgtatgtggg gcattaacat cgataaactg tacgaaaaac gacacataga acaaaatggc
    11341 tttcggatta gtcaggctca aaattaacgc gcgtttaaaa atggccccgt attggggctc
    11401 atcggatttg gcctcgttat ttttaccctt cagggtcgcg tagagaattt tactccccag
    11461 atagagcaaa taaaacgcac caagataacg tacaatgttg aataatatcg gggtggtctt
    11521 aattaatgtc gccactccag cccatgccag aaacatcaat actgcatcgc caataaatac
    11581 accgcaggcc gcaagataac cgcctttcat accgctactg acgctatttt tgagtacaaa
    11641 cagggtattt ggccctggca ccaacacaat aaaaatggcc ccaaccagat aggtccagta
    11701 attcagaacc ccgtattcag cgaacacatt aacctcttta attatctttc gatcatgcgc
    11761 gattaaaggt gaatatgcta accaatctgt agcggcttag aaaggagaaa atcaggtttt
    11821 aacccgatat caacccgata attgaatcat taatcaggca tgctccagtg aaaaattcgg
    11881 gtagtgctcg gcaaaatact ggcgtaaaaa ttctaccgtt atccgcactt tcgctgacgt
    11941 cgccagcctt gaaacataaa cggcccagac gttcgctggc tggtaatatt ccggtagcac
    12001 ttgcactaaa tgaccactgg caatgttttc gctaacatcc caccaggagc gcagagcaat
    12061 cccctgccca tcgagacacc actgatggac aatttctcca tgattagacg acagtgggcc
    12121 ggtgacttta atcgcatggt ggccttcttt attgcgtaat tgccagacac caaaaggatg
    12181 atcgcgctct ttaatgacca ggcacggcaa tgcggaaaga tcggttaaat gtttcggcgc
    12241 gccatgttgc gcaataaatt ccggcgacgc gcagagaatg cggtaattag tcgccagttt
    12301 gcgggcgatt aaattagggg cgatgtcatc gccaatgcga atatcgagat caacaccttc
    12361 attgaccaaa tcgaccaacc ggtcttccac atcaaaacgt aattccagtt gcggatacgc
    12421 cttagccagc gctgataacg ccggagccac cacctgtcgt ccaaaaccaa agctgctgat
    12481 aatacgcagc atcccctgcg gcacctgacg cacgtcagaa agttcgtcca tcatctgacc
    12541 gacatcctgc aaaatccgct gcgcccattc ataaatccgc tctccttctt cggtaatggt
    12601 gacgcggcgg gtggtgcggt gtaacaacac cacgtttagc gtttgctcca gcaaggcgat
    12661 gcgcttgctg acgaacgccg gtgaaacgcc cagttcttcc gccacggcgg caaaaccagc
    12721 ccgacgagcc accagcataa agacgcgcaa atcattcagc agcggtaaat tattcatgat
    12781 tcgtgtttta tgtttcacca gttacgggga ttaattcctt ttcagtcaat tataggatgg
    12841 tgatgttgtc aattttgatg gtcaggaagt gagaacccaa tgatgaaaac gatgcgtatt
    12901 gctgcgatcc cgggagacgg gattggcaaa gaagtccttc ctgaagggat tcgcgtgtta
    12961 caggctgccg ctgagcgctg gggcttcgcc ttgagttttg agcaaatgga gtgggcgagc
    13021 tgcgagtatt acagccatca cggtaaaatg atgccggacg actggcatga gcaacttagc
    13081 cgtttcgacg ccatctattt tggtgccgtc ggctggccgg acaccgttcc tgaccatatt
    13141 tcgttgtggg gttcgctgct gaaatttcgt cgtgaattcg accagtacgt caacctgcgc
    13201 ccggttcgtc tctttcctgg cgttccctgc ccgctggcgg gaaaacagcc tggcgacatc
    13261 gatttttacg tggtcaggga aaacaccgaa ggcgaatatt cctcgctcgg cggtagagtg
    13321 aatgaaggta cagagcatga agtcgtcatt caggaatcgg tatttacgcg tcgtggtgtc
    13381 gatcgcattt tgcgttatgc cttcgaactt gcgcaaagcc gcccacgtaa gacgctaact
    13441 tctgccacta aatcaaacgg tttagccatc agcatgccgt actgggatga gcgagtggaa
    13501 gcaatggccg agaattaccc ggagatccgc tgggacaagc agcatattga tattctctgc
    13561 gcgcgttttg tgatgcagcc ggaacgcttc gatgtggtgg tggcgtccaa tttatttggc
    13621 gatatccttt ccgatcttgg cccggcctgc accggcacca ttggcattgc cccatccgcc
    13681 aacctgaatc cggaacgcac tttcccgtcg ctcttcgagc cagtccacgg ttccgcgccg
    13741 gatatctacg ggaaaaacat tgctaaccct atcgccacga tttgggccgg tgcaatgatg
    13801 ctcgattttc tcggcaatgg cgatgagcgt ttccagcaag cgcataacgg tattctggca
    13861 gcaattgaag aagtgattgc tcacgggccg aaaacacctg atatgaaagg caatgccacc
    13921 acgccacagg ttgccgacgc gatttgcaaa attattttgc gttaaggtca aaccagttta
    13981 tttgaaccgc gtcactgacg cggttttttt attcgttctt tgcagtaaat aacctgcgtc
    14041 atttcacctt ttattgtttc cgtttcgtgt tttatggctt tccgtattct taattgttta
    14101 atttatgtaa catgcaaata atcaatttgt taaacacaac atacaactca ttaccctgca
    14161 aataattaca actggcaaaa gccagagacg ggcttttatg cccaggagat gactcatgat
    14221 gagcaatgtt aagaaaaaag atgtgccgct gataagcatc agcctggtgg ccattctttt
    14281 catcgcagct gcattaagcc ttttcccaca acaatcggcc gacgcggcca acgcaatata
    14341 cacttttgtt acgcgtacgt taggttccgc cgtacaggta ttggttttgc tggcaatggg
    14401 actggtgatt tatttagcca ccagtaaata cggcaatatt cgtcttggcg aaggaaaacc
    14461 ggaatacagc acgctctcct ggctgtttat gtttatttgt gccggtttag gttcttctac
    14521 gctttattgg ggggttgctg aatgggccta ttattatcaa acgcctggat taaatatcgc
    14581 accgcgttca caacaggcac tcgaatttag cgtcccttac tccttcttcc actggggcat
    14641 cagcgcctgg gcaacttata cgctggcctc attaatcatg gcttatcact ttcatgtgcg
    14701 taaaaacaaa ggtctgagtc tttccggcat tattgccgcg attaccggcg ttcgcccgca
    14761 aggcccatgg ggaaaactgg ttgatttgat gttcctgatc gccactgtcg gcgcactgac
    14821 tatttccctt gttgtcaccg cagcaacctt tactcgtgga ctttccgcgc tgaccggttt
    14881 acccgataac ttcaccgtgc aggcatttgt gatcctgctt tccggcggca ttttttgcct
    14941 aagctcgtgg attggtatca acaacggttt gcaacgtctg agcaaaatgg ttggctgggg
    15001 cgcgttcctg ctgccattac tggtgctgat tgtcggccca accgaattta ttaccaacag
    15061 catcatcaat gccatcggtc tgaccacgca aaacttcctg caaatgagct tattcaccga
    15121 tccgcttggc gatggttcat ttacccgcaa ctggaccgtt ttctactggc tgtggtggat
    15181 ctcatacacc cctggcgtag caatgtttgt cacccgcgtt tcccgcggtc gtaagattaa
    15241 agaagttatc tggggactga tcctcggcag caccgtcggt tgctggttct tcttcggcgt
    15301 aatggaaagc tatgccattc atcagtttat caatggcgta atcaacgtcc cacaggtgct
    15361 ggaaacactg ggcggcgaaa cagctgtaca gcaagttctg atgtcgttgc cagccggtaa
    15421 attgttcctc gccgcatacc tgggcgtgat gattattttc cttgcctcgc atatggatgc
    15481 ggtggcctac accatggctg cgaccagtac gcgtaatctc caggaaggtg acgatcctga
    15541 ccgtgggctg cgtcttttct ggtgcgtggt gatcactctg atcccgcttt ccatcttgtt
    15601 taccggtgct tcgctggaaa cgatgaaaac caccgtcgtg ctcacagccc ttcccttcct
    15661 cgtcatttta ctggtgaaag tcggcggatt tattcgctgg ctgaaacagg attacgccga
    15721 cattccggct catcaagttg aacattatct cccgcagaca ccggttgaag ccctggaaaa
    15781 aacaccagtg ctccctgcgg gaaccgtatt caaaggcgac aactgagcgc catcatccta
    15841 acgataaagg tatccctatg agcaatctga gccctgactt tgtactaccc gaaaattttt
    15901 gcgctaaccc gcaagaggcg tggaccattc ctgcccgttt ttataccgat cagaacgcgt
    15961 ttgaacacga aaaagagaac gtcttcgcca aaagctggat ttgcgtcgct cacagcagcg
    16021 aactggcgaa tgccaatgat tatgtgacgc gtgagatcat tggcgaaagc atcgtgctgg
    16081 tacgcggtcg tgataaggtt ttgcgcgcgt tctataacgt gtgtccgcac cgtggtcatc
    16141 agttgttgag cggtgaagga aaagcaaaaa atgtgattac ctgcccgtat cacgcatggg
    16201 cattcaaact cgatggcaat ctggcccatg cacgtaactg cgaaaacgtc gccaatttcg
    16261 atagcgacaa agcgcaactg gttccggtgc gtctggaaga atatgccgga ttcgtcttca
    16321 tcaacatgga ccccaacgcc accagcgtag aagatcagtt acccggtctg ggcgcgaaag
    16381 tgctggaagc ctgcccggaa gtccacgatc tgaaactggc ggcccgcttt accacccgca
    16441 cgcctgccaa ctggaagaac attgtcgata actatctcga gtgctatcac tgtggtccgg
    16501 cgcatccagg tttctccgac tccgtacagg ttgatcgtta ctggcacacc atgcacggta
    16561 actggacgct gcaatacggt ttcgccaaac cgtccgaaca gtcgtttaaa tttgaagagg
    16621 gtacggatgc ggcattccac ggtttctggc tgtggccgtg cacgatgctg aacgtcaccc
    16681 cgatcaaagg gatgatgacg gtcatttatg aattcccggt ggattctgaa actaccctgc
    16741 aaaactacga tatttacttc accaatgaag agttaaccga cgagcaaaaa tcgctgattg
    16801 agtggtatcg cgatgtgttc cgtccggaag atttacgtct ggttgaaagc gtacagaaag
    16861 ggctgaaatc gcgtggctat cgtggtcagg ggcgcatcat ggccgacagt agcggtagtg
    16921 gcatttccga acatggtatc gcccatttcc ataatctgct ggcgcaggtg tttaaggact
    16981 aatgacatcg gcggcggtat tttccgccgc tgggctgatt tttgatggag tacagcaatg
    17041 tcagactatc aaatgtttga agtacaggtg agtcaggtgg aaccgcttac cgaacaggtg
    17101 aaacgcttca cgctggtggc aaccgatggc aaaccgctgc ctgcgttcac cggaggaagt
    17161 cacatcattg tgcagatgag cgatggtgat aaccagtaca gcaatgcgta ttcactactg
    17221 agttcgccgc atgacacctc ttgttatcag attgccgttc ggctggagga aaactcgcgc
    17281 ggcggttccc gctttttgca tcagcaggta aaagtgggcg atcggttaac gatttcaacg
    17341 cctaataacc tgtttgcgct aattccctca gccagaaagc atctgtttat cgcgggcggt
    17401 attggtatca cccctttcct gtcgcacatg gcagagctgc aacacagcga cgtcgactgg
    17461 cagctacatt actgctcgcg aaatccagaa agttgcgcat ttcgtgatga gctagtccag
    17521 catccgcagg ctgagaaagt ccatttgcat cattcatcaa ccggaacacg actggaatta
    17581 gcgcgattat tggcggatat cgaacctggc acacacgttt atacctgtgg ccccgaggcg
    17641 ctaattgaag cggtaagaag tgaagctgcg cgtctggaca tcgccgccga tacgctgcac
    17701 tttgagcaat ttgctatcga agacaaaacc ggcgatgcat ttaccctggt gcttgcccgt
    17761 tccggaaaag agtttgtggt gccggaagag atgactattt tgcaggttat tgaaaataat
    17821 aaagccgcga aagtggaatg tttatgtcgt gaaggggtat gcggaacctg cgaaacagca
    17881 atactggaag gtgaagctga ccatcgggat caatatttta gcgatgaaga gcgtgccagc
    17941 cagcaaagta tgttgatctg ttgttcgcgt gcgaagggta aacgcctggt gttggatttg
    18001 tagtttgccg aagccggatg tggcgctgag cgcgcccagt ccggcttcgg aagattttac
    18061 tgcggatatt cctgcaataa attgtgtaat gcttccgcca tcagctcacc acgccagccg
    18121 gaaatcagct ccggcaaatt gttctgcggt ttcagtttcc agtgccagtt cagcagttgg
    18181 ttgatttgcc gacgcgatgc cagcaattcg gcgctgatct tatgcgtttc gctcacgtca
    18241 gtaatcagcg acttaatcgc tttaaacgct ttacgataac ccggcatgtc catcaggtta
    18301 agcatcggct gcggtaaggc ctcttccggc aatgtctgcg ctttttccac cagcgccagc
    18361 agcgttttac cgtgaaagcg gatttcgcta ccggataaac ccaggctgtc cagttcgcct
    18421 aaacttcccg gcatataacg cgctaccgac cacaaatgct cttcacgcac gacaaagttc
    18481 accgccagat cgcgctctcg cgccttgcgc agtcgccagt cggctaacag ttgcagacag
    18541 gccagttggc gtgtgcgtaa ttgccaggca ttggtgatat cacgccaggc atcttccggc
    18601 gcaacgactt cctgacgacg catttgcatc aggcggcatt catccagcgc cgcaggtagc
    18661 cagccggagg cctccgtttc taccataagc ttggcggtga tcggtaacag ataccagaca
    18721 tccgccgctg cgtattcaca ctgacgttcg gtcagcggtc tggccagcca gtcggtgcgc
    18781 gattcactct tgtccagcgt aacgccggaa tactcttcca ccatggaagc gaaaccccat
    18841 gacatcgggc gtccgcagaa ggcagcaagg atttgcgtgt caatcaaggg ttgtggtaat
    18901 tcgccaaaga cattgaggaa cacttccaga tcttcactgc ctgcatggag aaattttgtg
    18961 atggacggat cgcgcaggat cgctttcagc ggtgaccagt cggtgatccc gagaggatca
    19021 attagcgcca gatgctcgcc atcgaaaagt tgaatcaacc ccagctgcgg gtaataagtg
    19081 cgcgtacgaa caaattcagt atccagggct atcgccggaa aggcacggac ggcttcacac
    19141 aaagaagcca gcgcatcgtc cgtggtaatc atttggtaat tcaaattgtt ttctctttag
    19201 tgggcgtcaa aaaaaacgcc ggattaaccg gcgtctgacg actgacttaa cactcaggct
    19261 ttattgtcca ctttgccgcg cgcttcgtca cgtaattctc gtcgcaaaat ttttccgacg
    19321 ttagatttcg gtaactcatc acgaaactcc accagcttcg gtactttgta gcccgtgagc
    19381 tgacggcggc aaaaggtcac cagtgactct tcggtaagcg atggatcttt tttcactacg
    19441 aagattttca ccgcttcacc actggagccg gaaggtacgc caacagccgc gacttcctgt
    19501 acgccaggat gctgcatgac gacatcttca atctcgttgg gatagacgtt aaaaccggaa
    19561 accagaatca tgtctttttt acgatcgaca atgcgcagga acccttcttc atccatcacc
    19621 gcaatgtcgc cggtgtgtaa ccagccattt ttgatgatct catctgtagc atccggacgc
    19681 tgccagtaac ccagcatcac ctgcggtcct ttgacacaaa gctcacccgg ttgacccggt
    19741 ggtacttcat tatcatcatc atccaccagt ttggcttccg tcgacggcac cggcaaaccg
    19801 atgctaccac tatgataatc aatatcatat gggttaacgc tgaccagcgg cgcacactcg
    19861 gtaaggccat agccttccag cagatactgt cctgtcagtt tcacccaacg ctctgccacc
    19921 acttgctgca ctggcatccc tccgcctgcg gaaagatgca gactggagaa atccagctgc
    19981 tggaactctt tattgttcag caacgcattg aacaaggtgt taacgcccgt gatagcggta
    20041 aacggatatt tcgctaactc ttttaccaac cctggaatat cgcgcgggtt agtgataagc
    20101 aggttctgcc cacccagttc gataaacagc aggcagttaa tggtcagggc aaaaatgtga
    20161 tacagcggca gcgccgtcac caccagctct ttgcccggat gcaacagcgg accataggtg
    20221 gcgttgacct gttccaggtt cgccagcata ttgcggtgag tcagcatcgc gcctttcgcc
    20281 acacctgtgg tgccgccggt gtattgcagg aaggctaaat cttccggcac cagttcgggt
    20341 ttgacgtact gcatccggta gccgttatgc agtgcgctac gaaatgaaat ggcatctggc
    20401 agatggtatt tcggcaccaa acgcttgatg tatttaacaa cgaaattgac taccgtgcct
    20461 tttgccgtag atagctgatc gcccatacgg gtcagaatta cgtgctgaac ggcggtttta
    20521 tcaaccactt tttccagtgt gtgagcaaag ttagacacga taacaatcgc cgatgcgccg
    20581 ctatcgttaa gctgatgctc aagctcacgc ggggtataca acgggttaac gtttacgacg
    20641 atcatcccgg cacgcaaaat gccaaacagc gccaccggat attgcaataa attaggcatc
    20701 atcaacgcaa cgcgatcgcc tttcttcagc cccaaccctt gttgcaaata agcggcaaac
    20761 gcgcgactgc gttcttccag cttgcggaag gtcattacct cccccatatt cacaaacgca
    20821 ggctgatcgg cgtagcgcgc gaccgactgc tcaaacatat ctaccagaga ttgataacgg
    20881 tcagggttga tctccgtcgg aacgtccgcg ggataacggt taagccaaac cttcttcaat
    20941 tcttcacctc taaaatgcgt gttcgtcgtc atcgcaaccc caaatgatat acatgccgtt
    21001 aacataatat taactcatca taccagcttg ataattaccc aacgaaaagg ttgcgaagcg
    21061 cgtcactatt tatttttatc tttaccgtaa gaatgcagaa acagcggacc agccgctgtt
    21121 tctttttctt taaaaacaag caattattca gttacaactg tttgtactct cgcggggccg
    21181 ggattatacc agccccatcc gccatagcca tagggccagc cacgaccgcc ataaaaccat
    21241 ggatcaatcg gctgaggcgg cataatcacc tgctgggtta aatgccaacg tttgtaaccc
    21301 gttacttgca tcaccataaa tttatagggc gtgttgccga tttttccgtc aaccgcacca
    21361 gtgattggcc cgactaccgt aaccagttgt ccacggaaat ccaccgggtc cagaaaaccg
    21421 ttcatatcgg cataaatgcg accgcgagaa ggttctccca gcgtcggtct ggctccgctg
    21481 tccagcggta cggtagcaat ttccaggcgg gttttccctt gctggttttg taccgcaacc
    21541 actttgccac caaagcgtgc ctcctgacca acgtacagct gcggcgcact catcacccga
    21601 actaaatctt gttgcggcgt gggactgctg cctttaatgg cgtccggcac agtgacacaa
    21661 ccgctcagca ttagcgcaaa cgtacctgcc agtatgcctt tgataacatt cttttgaacc
    21721 gccatggtgc gactcctttt tttctcaggg catactctta agattcattc tttgcccgga
    21781 agtttcttcc atgcgacgtt gttacgtaaa taaaccggtt cggcatgttc caccgccacc
    21841 gttttaccct cggcaaacat ctgacacgca atcggcagca tatcttcagc agcaggcagt
    21901 aacacttcgc catcgcgcaa aaccagcccg ctctctttac cgagatccgg ccaggcttgc
    21961 cagcccgtcc ctaccgtcac ccattcgccg gaaagctgtt gcattcgttc atggacgatt
    22021 tcgggtttga gtacggcttc ggtttcttca ccgtgccaga taccgttttc atcacgctga
    22081 tattcggccc agtaaacttc gcccattcgc gcgtcaatgg ctgacagcac gcgggttgcg
    22141 ccgtttttgc gccacgcccc ttgcgccatc gtcattagcg tggagacgcc aatcatcggt
    22201 aattccgcgc caagcgccag cccttgcgcg atgccaatac caatgcgcac gccggtaaag
    22261 ctaccggggc cgcgcccgta agccagagcg tttatgtcag tcagggaagt tccgctggtg
    22321 gtcaggatat cctgcaccat cggtaagatt cgttgagtat gttcacgagg gcaaagctca
    22381 aaatgagcgt tgacagtacc gtcgttccac agggcgacag agcaggcctc tgtcgcggta
    22441 tcgatagcca gaattcgcat gggtcttcgt gcttttttca ataaaaaggc gcgcatcata
    22501 ccatactccg taacaaatta cctggaggat ggtatcgcaa ggaaacgaac cgcacgggca
    22561 atgtcacggg tgcgcggcgc gggcggcaga ctggcgagaa acgtcgcgcc gtaagggcgc
    22621 atcaccagcc gattgtcaca aatcaccaaa accccgcgat cgtcggcgtc gcgaatcagt
    22681 cgccctaccc cctgcttgag agtaatcacc gcatccggca gttgtacttc atcgaacggg
    22741 tcgccaccgc gcaaacggca atcttccatg cgcgctttta acagcgggtc atccggtgag
    22801 gtaaacggca acttgtcgat aatcaccaat gacaatgtat cgccacgcac gtccacccct
    22861 tcccagaaac tactggttgc cacaagaagc gcgttaccgg cgctgacaaa ttgctgcaac
    22921 agttgccctt tgctggtttc cccctgcaac aaaacgggaa gcgtcatggt agcgcggaac
    22981 tgctcggcca gatcacgcat catggcgtgc gaggtacaaa gcataaaaca acgaccgttg
    23041 ttagcttcga tgatcggtcg cagcattgcc gctaactggc gagcagaacc tggctggttg
    23101 gtttgcggca gattgcgcgg cacacagagt aacgcctggc ggctgtaatc aaacgggctt
    23161 ggcaatagca acgactccgc ctgttctatc ccaagccgcg aggtgaaatg atgcagatcg
    23221 tcgttcaccg acagcgttgc tgaggtgaag atccagctac caggcttttg cgccattaac
    23281 tctttgaatt tatccgccac gctgagcggc gtgagagcca gagtaaaatg gcgcgaagtg
    23341 cattcgtacc agtagctgta gcccggctga ttgatctctt ttagccgctt cagccgtgtg
    23401 cgatacaacg tagcgcgctc aaatgccgca tccagcaagg cggaacgccc cagtgacagt
    23461 ttcgccacgt cataacaaag ttccagggtg tcatcgagca gtaaaaatgc ccgctgaatt
    23521 tgcggattag ctaacagctc acgcaggtta ccgcgataac ctggctcacc gagttgcaga
    23581 cgaaaatcct gcgcactctg ggcaagacga tcagcgcact tttgtaactg ctgggtgtct
    23641 tttaattcgg tgcggtaagc gatggtgatg tcttttgcca ggtcgagcag ttgtcgactg
    23701 gagagtgact gaccaaaata ctggctggca atgtccggta gctggtgggc ttcgtcgaag
    23761 atcatgacgt ccgcttccgg gatcagttcg ccaaatccac tctctttaac caccatatcc
    23821 gccagaaaga gatgatggtt taccaccacc acatcggcgt ccatcgcttt tttacgtgct
    23881 ttgaccacaa agcaatcttt atacatcggg cagtcgctgc caagacagtt gtcgttggtg
    23941 ctggtgacca gcggccacgc ctgtgaatct tccgccacgc tgacgcaggt gctgatatca
    24001 ccatcgactg tttgattaga ccaggagcgc agcaggatca catcgcttaa gatttgtacc
    24061 ggcagatcgc cccccgccag cgcctgctgt tcgagacgtt cgaggcagag gtagtttgag
    24121 cgccctttca gcagcgccac gttgcccgta tatttcaatg cctttgagac tgttggcaaa
    24181 tcgcggctgt agagctgatc ctgcaacgct tttgagccgg tcgagataat gactttcttt
    24241 ttcgcccgca gcgcaggagc caggtaagcg taggttttgc ccgtaccggt tcctgcttcc
    24301 accaccagcg gctggccttt ttctatcgcc tgggtgacgg ctaccgccat ctgtcgctgt
    24361 ggttctcgcg gcttaaagcc tggtatcgct ttcgccagct gaccgtctgg tgcaaaatcg
    24421 tccgtcacac taccccctgt tgatttgaac agggattatg tcaggatgag ggcgctttcg
    24481 ccagttgaag tggtgacggc gacctcacat tgtggcagtc tttgcagcac gaaaatggaa
    24541 ggataatgag gacaaaatga tgactatcgt tcgtatcgat gctgaagccc gctggtctga
    24601 tgtggtaatc cacaacaaca cgctctacta cactggtgta ccggaaaacc tcgacgccga
    24661 tgcctttgag caaaccgcca acacgctggc gcagattgat gccgtgctgg aaaaacaggg
    24721 cagcaataaa tcgagcattc tggatgccac cattttcctg gccgataaaa acgacttcgc
    24781 ggcgatgaat aaagcgtggg atgcctgggt tgtcgcgggt catgcgccgg tgcgctgcac
    24841 ggtacaagcg ggtttgatga acccgaagta taaagttgaa attaagattg tggctgcggt
    24901 gtaagcttta tcgaagcaaa ataagtcaga cgataattta tcgataatac tggtcggttt
    24961 tacataaatc gaccagagat taagatatta cctgttgatg ctcctgctaa ttcattattt
    25021 atttttataa tttcaatttt at
//
LOCUS       DAZGVQ010000068        25007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000068 DAZGVQ010000000
VERSION     DAZGVQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.068"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            78..305
                     /locus_tag="ACHLAG_002636"
     CDS             78..305
                     /locus_tag="ACHLAG_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655841.1"
                     /translation="MACNVFAFSISGGVSERSYKETEKTSAMTTTHSTKLQPSQAILF
                     KMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(382..624)
                     /gene="yfdY"
                     /locus_tag="ACHLAG_002637"
     CDS             complement(382..624)
                     /gene="yfdY"
                     /locus_tag="ACHLAG_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFP0655842.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            977..1897
                     /gene="lpxP"
                     /locus_tag="ACHLAG_002638"
     CDS             977..1897
                     /gene="lpxP"
                     /locus_tag="ACHLAG_002638"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFP0655843.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFINPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2253..2324
                     /gene="ypdK"
                     /locus_tag="ACHLAG_002639"
     CDS             2253..2324
                     /gene="ypdK"
                     /locus_tag="ACHLAG_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFP0655844.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2389..3627)
                     /gene="alaC"
                     /locus_tag="ACHLAG_002640"
     CDS             complement(2389..3627)
                     /gene="alaC"
                     /locus_tag="ACHLAG_002640"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFP0655845.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            4004..5701
                     /gene="ypdA"
                     /locus_tag="ACHLAG_002641"
     CDS             4004..5701
                     /gene="ypdA"
                     /locus_tag="ACHLAG_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFP0655846.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDGFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            5716..6450
                     /gene="ypdB"
                     /locus_tag="ACHLAG_002642"
     CDS             5716..6450
                     /gene="ypdB"
                     /locus_tag="ACHLAG_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFP0655847.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6463..7320
                     /gene="ypdC"
                     /locus_tag="ACHLAG_002643"
     CDS             6463..7320
                     /gene="ypdC"
                     /locus_tag="ACHLAG_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0655848.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7323..9818)
                     /gene="ptsP"
                     /locus_tag="ACHLAG_002644"
     CDS             complement(7323..9818)
                     /gene="ptsP"
                     /locus_tag="ACHLAG_002644"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP0655849.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEVTFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAALFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9843..10880)
                     /gene="ypdE"
                     /locus_tag="ACHLAG_002645"
     CDS             complement(9843..10880)
                     /gene="ypdE"
                     /locus_tag="ACHLAG_002645"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP0655850.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYSAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(10880..11965)
                     /gene="ypdF"
                     /locus_tag="ACHLAG_002646"
     CDS             complement(10880..11965)
                     /gene="ypdF"
                     /locus_tag="ACHLAG_002646"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP0655851.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(11980..13227)
                     /gene="fryC"
                     /locus_tag="ACHLAG_002647"
     CDS             complement(11980..13227)
                     /gene="fryC"
                     /locus_tag="ACHLAG_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP0655852.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13249..13575)
                     /gene="fryB"
                     /locus_tag="ACHLAG_002648"
     CDS             complement(13249..13575)
                     /gene="fryB"
                     /locus_tag="ACHLAG_002648"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFP0655853.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAVGIIKEIEEM
                     IASEQQ"
     gene            complement(13794..14759)
                     /gene="glk"
                     /locus_tag="ACHLAG_002649"
     CDS             complement(13794..14759)
                     /gene="glk"
                     /locus_tag="ACHLAG_002649"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFP0655854.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            14963..16219
                     /gene="yfeO"
                     /locus_tag="ACHLAG_002650"
     CDS             14963..16219
                     /gene="yfeO"
                     /locus_tag="ACHLAG_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFP0655855.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASALQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            16334..16660
                     /gene="ypeC"
                     /locus_tag="ACHLAG_002651"
     CDS             16334..16660
                     /gene="ypeC"
                     /locus_tag="ACHLAG_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFP0655856.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(16801..18039)
                     /gene="mntH"
                     /locus_tag="ACHLAG_002652"
     CDS             complement(16801..18039)
                     /gene="mntH"
                     /locus_tag="ACHLAG_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFP0655857.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            18375..19577
                     /gene="nupC"
                     /locus_tag="ACHLAG_002653"
     CDS             18375..19577
                     /gene="nupC"
                     /locus_tag="ACHLAG_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFP0655858.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(19627..21816)
                     /gene="pdeA"
                     /locus_tag="ACHLAG_002654"
     CDS             complement(19627..21816)
                     /gene="pdeA"
                     /locus_tag="ACHLAG_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFP0655859.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQSVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(22024..22099)
                     /locus_tag="ACHLAG_002655"
     tRNA            complement(22024..22099)
                     /locus_tag="ACHLAG_002655"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22064..22066),aa:Ala,seq:ggc)
     gene            complement(22139..22214)
                     /locus_tag="ACHLAG_002656"
     tRNA            complement(22139..22214)
                     /locus_tag="ACHLAG_002656"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22179..22181),aa:Ala,seq:ggc)
     gene            22435..22794
                     /gene="yfeC"
                     /locus_tag="ACHLAG_002657"
     CDS             22435..22794
                     /gene="yfeC"
                     /locus_tag="ACHLAG_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP0655860.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            22796..23188
                     /gene="yfeD"
                     /locus_tag="ACHLAG_002658"
     CDS             22796..23188
                     /gene="yfeD"
                     /locus_tag="ACHLAG_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP0655861.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(23240..24655)
                     /gene="gltX"
                     /locus_tag="ACHLAG_002659"
     CDS             complement(23240..24655)
                     /gene="gltX"
                     /locus_tag="ACHLAG_002659"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFP0655862.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            24914..24989
                     /locus_tag="ACHLAG_002660"
     tRNA            24914..24989
                     /locus_tag="ACHLAG_002660"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24947..24949,aa:Val,seq:tac)
BASE COUNT         6232 a         6615 c         6148 g         6012 t
ORIGIN      
        1 tcagtgaacc attttcagga taactttcta tgaaagtaaa cttaatactt ttcagcttat
       61 ttttattggt ctctattatg gcatgcaatg tttttgcatt ttccatttct ggtggtgtaa
      121 gtgagaggag ctataaagag actgaaaaaa catcagcgat gacgaccaca cactctacaa
      181 aacttcagcc atcacaggcg attttgttta agatgagaga agatgcgcca ccattaaacc
      241 tcacagaaga aatcacgcct acttatccga caaaggcgaa ttatcttatt catcctgtgc
      301 gatagcgtct gtcacatggc aggtgaacaa tcatgtctga tgctgatgtg aacacctgcc
      361 caaatgagcc atcctgaccc actatcgcct gccatacccc accataaagc gaatcatcaa
      421 gccacaagcc ggggcaagaa acataaatat ccccgtcact agttcattac cgccagttaa
      481 ccacatggtg aagtaataaa tgagtgcagc gaggatcacc atgccgaaga aagaagagac
      541 tgccgatacc acgttcagta cctgaccgat gtagccgctt acgcagacaa tacacagggc
      601 gagaaacatc cataaataaa tcatagctca acctcctgcc gcgctaaaaa acctgtaact
      661 tcagtcactt taatagtaag acaatcctta acctctggca atttttgcta agcatcaatg
      721 aaaacagcat gttaaatgca agactgttgt gtacggaaaa atatttactt tgcacgatta
      781 ataatcagta gctgaaagca gtcagcgagt gataaagtac agataccgca ttcatgctcc
      841 tcgtggttat gtcctgacta gtctttacac tctttacagg aaccattgtc gtacatgatg
      901 gcccaaccaa ttgagtgtac cgctgtcgac atggaatctt ctgcgataca acaattcgta
      961 tctacagaag gtaactatgt ttccacaatg caaattttcc cgcgagtttc tacatcctcg
     1021 ctactggctc acatggtttg ggcttggtgt actctggctt tgggtacagc ttccttatcc
     1081 tgttctctgc tttctcggca cgcgtattgg cgcaatggcg cgaccattcc tgaaacgtcg
     1141 tgaatctatc gcccgtaaaa acctggaact ttgtttcccg cagcattctg cggaagaacg
     1201 cgagaagatg attgccgaaa actttcgttc actcggcatg gcgctggtag aaaccggcat
     1261 ggcatggttc tggcccgaca gtcgcgtacg taaatggttt gatgttgaag ggttggataa
     1321 ccttaaacgc gcacaaatgc aaaatcgcgg cgtaatggtt gtcggcgtcc attttatgtc
     1381 gctggaactg ggcggccgcg tgatgggact gtgccaacca atgatggcta cctatcgtcc
     1441 acataataat cagctgatgg aatgggtgca gacccgtggg cgcatgcgct ctaacaaagc
     1501 gatgatcggc agaaataatc tgcgcggcat tgtcggtgca ctgaagaaag gtgaagcggt
     1561 atggtttgct cccgatcagg attatggtcg taaaggcagc tccttcgcgc cgttctttgc
     1621 ggtggaaaat gtcgccacaa ccaatggcac ctatgttctc tcccgtctct ctggcgcagc
     1681 catgttgacc gtaacgatgg taagaaaagc ggattacagc ggatatcgtt tgttcatcaa
     1741 cccagagatg gaaggctacc cgacagatga aaatcaagcc gctgcctata tgaacaagat
     1801 tatcgagaaa gagatcatgc gcgcaccgga gcagtacctc tggatccacc gtcgctttaa
     1861 aacgcgcccg gtgggagaat cgtcgttgta catttaacaa actgctctta ctctttccct
     1921 cctcaaatcg gccataataa cctcatggtt tttatggctt ttttcttaag gttttttcct
     1981 tcgctgaata tctaattatt accgtcagaa attaaactgt cgccgatatg agacacttgt
     2041 aagttactaa aagtaaataa gaaaatgcct cttgtctcgt ttcagtttta ggcgtacatt
     2101 agcgccgtct cgagcagttt agctcagaat tctggggtga tgtccacggt aaaatacagg
     2161 gcttttaagg tcgctttgcc cggtgtcaac tcactgtatc aggtgtaatg aagtcattca
     2221 ggcgtaacag taattacgcg gagagatgta aagtgaaata tttctttatg ggcatttctt
     2281 ttatggtcat cgtttgggcc ggtacttttg ccctgatgat ctaaaagcaa actgtgcaat
     2341 gaaaaacagg agccatcagg ctcctgtttg catttctgac gacggtgttt attccgcgtt
     2401 ttcgtgaata tgtttgctgc tggcgggtag taaaccgtcg gcacggaaca tcgctttaat
     2461 ccctctgatg gcctgacgaa tacggtcgcg gttttcaatc agtgcaaagc gaacatgcgt
     2521 atcaccatag tcgccaaagc caatccccgg cgagacacaa accttcgctt cgttaaggag
     2581 cttcttggca aattccagcg atcccatggc cgcatatggt tccgggattt tcgcccagac
     2641 atacatcgaa gccttcggca tttcgaccat ccagcccgct tcatgcagcc ctttaaccaa
     2701 tacatcacgg cggcgtttgt actgttcggc aatgtcgcgc acgcactgtt gatcgccctc
     2761 gagtgccgca atcgccgcca cctgcaacgg cgtaaaagtg ccgtaatcgt gatagctttt
     2821 gattcgcgcc agggcactga ccagcgtttt gttgccgacc ataaagccga tacgccagcc
     2881 cgccatgttg tagcttttcg acagcgtaaa gaactcgact gccacatcgc gtgcacccgg
     2941 tacctgcata attgacggcg ctttccagcc atcgtagacg atatcggcat aggccaggtc
     3001 atggaccacc agcacatcgt agcgtttcgc cagcgccact actttttcga agaactccag
     3061 ttccacgcat tgcgcggttg ggtttgacgg gaagccgagg atcatcatct tcggtttcgg
     3121 ataactttca cgaatggcgc gctccagttc gttgaagaaa tcgacacctt ccaccagcgg
     3181 cactgagcgt acctgcgccc cggcaatcac cgcgccataa atatggatcg ggtaacttgg
     3241 attcggcacc agcaccgtgt caccatgatc cagcgtcgcc agcatcagat gcgccaggcc
     3301 ctctttcgaa ccaatagtga cgatggcttc tgattccggg tcgatttcaa cgtcgtagcg
     3361 atcctgatac cagcgggaaa tggcgcgacg taaccgcgga atgccgcgtg aagtggagta
     3421 accatgcgtg tccgggcgct gggccacagt acataatttt tcgacgatat gcggcggagt
     3481 cgcaccgtcc gggttaccca tgctgaaatc gataatatct tcgccgcgcc gacgcgcagc
     3541 cattttcagt tcagcggtga tgttaaaaac gtagggcggg agacgatcaa tgcgcgtaaa
     3601 gcgacgttca gggcgagtgt cagccataat ttcctcagat taacgttagc gcccggaccg
     3661 tccgagcgac gctgccacaa tggtggctgt tttgaaaata gcctgattaa tttctgtctg
     3721 tccagcgttt tagaacattt ttatcaatgg ctttaaatga ggaactccac ttcaaaaaca
     3781 ggaaattttt ctacgctaac aagaaaatta ccaccaattt tattaacatt ataatttcaa
     3841 taaatttacc agccagaagt gcattatcaa gagatacatt gctaatacac catttatctt
     3901 tcaatcccct tttaaattta tggtttttcc tcaaaaacgg cctgctggtc atcagccttc
     3961 aggttaccta tcatagaggt ttaatcctta ttcagagtca cccgtgcacg aaatattcaa
     4021 catgctgctg gcggtcttcg atcgggcagc gttaatgctt atctgcctgt tctttctcat
     4081 ccgtatccgc ctgtttcgcg aactgttgca caagtcggcg cactccccaa aagaattgct
     4141 cgccgttacc gccattttct cgctgttcgc cctgttcagc acctggtccg gcgttcccgt
     4201 agaaggctcg ctggtgaacg tacgtattat cgcggtgatg tccggcggga ttctgtttgg
     4261 cccgtgggta ggcatcatta ccggcgtgat tgcgggtatt caccggtatt taattgatat
     4321 cggcggcgtg acggcgatcc cctgctttat caccagcatt ctggcgggtt gtatatcagg
     4381 ctggataaac ctgaaaatcc ccaaagcaca gcgctggcgc gtcggtattc tcggcggcat
     4441 gttgtgtgag acgttgacca tgattctggt aattgtctgg gcaccaacta ccgcgttggg
     4501 gatcgatatc gtctctaaaa tcggcattcc aatgatcctc ggtagcgtct gtatcggctt
     4561 tattgtgctt ctggtgcaaa gcgttgaggg cgaaaaagag gccagcgccg cgcggcaggc
     4621 caagctggcg ctggatatcg ccaacaaaac gctacctctg tttcgccatg tcaatagcga
     4681 gtcattacgc aaagtttgtg aaattattcg cgatgacatt cacgccgatg cggtggcgat
     4741 taccaatacc gatcatgtgc tggcctatgt tggcgtgggt gaacataact atcagaatgg
     4801 cgatggcttc attagcccga ctacccgtca ggcgatgaat tacggaaaaa tcatcattaa
     4861 aaacaatgat gaagcccacc gcacaccaga gattcattcc atgctggtga tcccattgtg
     4921 ggagaaaggc gttgtgaccg gaacgctgaa aatttactac tgccacgcgc atcagatcac
     4981 ctcgtcatta caggaaatgg cggtcggtct gtcgcaaatc atctccaccc aactggaggt
     5041 ttcacgcgcc gagcagctac gtgaaatggc aaataaggca gagcttcgcg ccctgcaaag
     5101 caaaattaat ccccatttcc tgtttaacgc tttgaacgct atttcatcat caatccgtct
     5161 gaatccggat actgctcgcc agctgatctt taatctgtcg cgttatctgc gctataacat
     5221 tgaattaaaa gacgatgagc aaatcgatat caaaaaagag ctgtatcaaa ttaaagacta
     5281 tattgccatt gagcaggccc gctttggcga caagctgacg gttatctatg atattgatga
     5341 agaggtgaat tgctgcattc ccagcctgct gatccagccg ttggtggaga atgccattgt
     5401 ccacggtatt cagccttgca aaggtaaagg cgttgtcacc atcagcgttg cagagtgcgg
     5461 caatcgggta cgcattgcgg tgcgggatac cgggcacggc atcgatccaa aggtgattga
     5521 gcgggtcgaa gcgaatgaaa tgccgggcaa taaaattggc ctgctgaatg ttcatcatcg
     5581 cgtgaagtta ttgtatggcg aggggctgca tatccgccgc ctggaaccag gaacggaaat
     5641 tgcgttttac attcctaacc aacgcacccc agtcgcctca caggctacgt tattgctttg
     5701 agccggagtg atattgtgaa agtcatcatt gttgaagacg aattcctggc acaacaggaa
     5761 ctgagctggc taattaaaga gcacagccag atggagattg tcggcacctt tgacgacggt
     5821 ctggacgtgt tgaagttttt gcagcataac cgcgtcgacg ccatttttct ggatatcaat
     5881 attccgtcgc tggatggcgt gttgctggcg caaaacatca gccagttcgc ccataaaccg
     5941 tttattgtgt tcatcaccgc gtggaaagaa catgcggtag aagcgtttga actggaggcg
     6001 tttgactaca ttctcaaacc gtatcaggag tcacgtatta ccgggatgct gcaaaaactg
     6061 gaagcggcct ggcaacaaca gcagaccagc agtacgcctg ccgcgacggt aacgcgtgag
     6121 aatgacacca ttaatctggt gaaagatgag cgaataatcg tcacgccaat taacgatatc
     6181 tattacgccg aagcgcacga gaaaatgacc tttgtctata cgcggcgtga atcctacgta
     6241 atgccgatga acattaccga attttgcagc aaactgccgc cgtcgcattt tttccgctgc
     6301 catcgctcat tttgtgtcaa tctgaacaaa atacgcgaaa tcgaaccgtg gtttaataac
     6361 acctacattc tgcgactgaa agatctggat tttgaagtgc cggtcagccg cagcaaagtg
     6421 aaagaatttc gccagttaat gcatctttaa tcgggaactt tcatgaaagc accaggcttg
     6481 cccgccgatc aacaattttt cgccgatctg ttcagcggcc tggtgcttaa cccgcaacta
     6541 ctggggcgcg tctggtttgc cagccagcct gcctcgttac cggtgggcag tttatgtatt
     6601 gattttcccc gtctggatat cgtgctgcgc ggcgaatacg gcaacctgct ggaagcaaag
     6661 cagcagcgaa tggtggaagg agaaatgctg tttattccgg cacgcgcggc taatttaccg
     6721 atcaacaaca aaccggtgat gctgttaagc ctggtgttcg caccgacctg gcttgggtta
     6781 tcgttttacg atagccgcac cacgtcgttg ttgcatcctg ctcgccagat ccagcttccc
     6841 agcctgcaac gcggtgaagg tgaagcgatg cttaccgccc tcacccatct tagccgttcg
     6901 ccgctggagc agaatatcat tcagccactg gtgttaagtt tgctgcatct ttgccgtaac
     6961 gtggtgaata tgccgccggg caattcgcag ccgcgcggcg attttctcta tcacagcatt
     7021 tgtaactggg ttcaggataa ttatgcccag ccgctcaccc gcgagagcgt ggcgcagttt
     7081 tttaatatca cgcccaatca tctgtcaaaa ctgtttgctc agcatggaac gatgcgtttt
     7141 atcgagtatg tgcgttgggt gcgaatggcg aaggcgagga tgattttgca gaaatatcat
     7201 ctgtcaattc atgaagtggc acagcgttgc ggttttccgg atagcgacta tttttgtcgc
     7261 gttttccggc gtcagtttgg tctgacgccg ggagagtaca gcgcccgttt tcagggctaa
     7321 cgtcagaagg ttaattctgt ttccagcagc gtcaggatac tttgtgcatc ttgcgcggca
     7381 aacaacgact ggcggaagtt tttattcacc agtttacgcg ccagctgcga gaagactttc
     7441 acatgattca tcccttcgtt agcacccagc gtcagcatga tcaccagttc gacttcgccc
     7501 atttctgact gccagtcaat cggtttcgcc agccgggcaa tgctgatact ggaatgacgg
     7561 atccactgag atttggtgtg cgggatcgct acgccaaaac caacgccggt ggtaacaatc
     7621 tcttcccgct gccagacatc ttcttccagc tcaaacggat gttcagtgcg cccgttaacg
     7681 ccgaggttgc cgcacaggaa ctggatcgcc tgctctttat tgctaaaatc ctgatcaaca
     7741 aagatattct ccagcgccag cagtgggcga acgtcttctt ccggcgtaaa ggcggtgagt
     7801 aacgcttcaa tttcctgcgc actgcggcat tcacatgcct gacgcgccag ttcccgacac
     7861 gcctcgctat ccagttgacg aagctggctt ttcaccgccg gaatacgcgg gctactcata
     7921 ctcagctcat ccaggcccag cccaagcagt agcggcagat aacggctttc accgcccagt
     7981 tcaccgcaaa tgcctaccca tttgccccgc tgatgcgcag tggtaacaat ctgctgcaac
     8041 atgcgcagga acgatggcgt aatcgggtta tacagcggcg atacgcgcgg gttattacga
     8101 tcgaccgcat acagatactg ggtcatatcg ttagaaccga tactgaagaa atcgacctca
     8161 tcgcagaagt ggtcgatgat gtaacacacc gacggcactt ccaccatgat acccagagta
     8221 atcgtctctg catgacgcag gccatcgcgc ttaagctcaa cgatcgcttt ttggatctcg
     8281 cccttcaccc ataagatctg atcgaggctg tgaaccatcg ggatcatcaa ctgggcgttg
     8341 ccaaaactgg cagcgcgcaa aatcgcccgt agttgagtgc ggaacagggc agcaaattcc
     8401 gggtaaatac gtaccgcgcg atagccgagg aacgggttct cttcctgggg aatattcaga
     8461 taaggaatgc ttttatcgcc tccgatgtcc attgtgcgga agataatcgg cttgtcgccc
     8521 gctgccagta gcacctgctg gtaggcttca aattgctcct gctcgtccgg cgcgctgtca
     8581 cgatccatat acagcatttc ggtacggaac agcccgacgc cttccgcgcc gttggcaaac
     8641 gcgcctggtg cttccagagc ggtgccaata ttcgccgcga tatcaatacg cttgttatca
     8701 cgggaatagg ccagctgcgc ggcagcctgc gcctgttgtt tttggcgttt atccgccagc
     8761 gtctgcgcaa cctgataata accgctcacc gcgtcattcg ggttaatcgc cagcacgccg
     8821 cactgggcgt caagcaccgc cggttgcccg gcataacggg caatcgcatc cagcggcagg
     8881 ccgctcagta ccgggatcgc cgaggcgcgg gccagaatca gggtatgcga ggtgcgcccg
     8941 gttttctcca gaatcatgcc cgcaagattt ttcaaatcaa ggctcaaaaa ctggcttggg
     9001 gttaaatctt cagccaccag aatggtcggt ttttccagca ccagattgtt gcgcggcttc
     9061 agttccggcc aggtgatatg cagcaactgt tcgctaatat cgcgaatgtc gctaacacgt
     9121 tcgcgcagat aatcgctggc agaagcagaa agcttggcgc aaacctgctc catattgctg
     9181 atgatcgccg cccccagtcc ctgatgctgt tctgtcatca ggcgacggat attgcctgca
     9241 aattcatcat cctgaatcag cgacaaatga gcgctgagga tagttttgct ttcgccgtca
     9301 cgctcacgca attgctggtt cagttgctcg gcaagcgttg ccaggctgtg ctccagccgg
     9361 gtggaatctt gcgcactggc ggggattgcc cgataactgt cgaggctgtc gctttgtaac
     9421 agggtcagcg tacccacgcc gacgccgctt gccagcacat tgccgtacag taaatccggg
     9481 ttaaggcggc ttaatgaacg cggcagcgga tgcgccgtca gttctgcctg cgtaggctga
     9541 acgctgtcgc tgtcgataaa gcgcacctgg atgtactctt ccagcacgcg ccgcgcctgc
     9601 tcttcatcgc tgccgctaat gttcaggctg caactgtcat taaatagggt gccggtgcca
     9661 atcagcgcca gcgaactttt cgcatcggcc tttgcgttct ggcgatggtt aataaaggtg
     9721 acttcacttt gccactggct gcactgttct ttaagttccc acgccggacg agcatgcaga
     9781 ccgttaggca aaggacagag aaattgaatc gttaacataa ccgctccggg ttaatcagga
     9841 gatcatctga aatccgtcag ttgaacaacc gtctcacggg taagacgttg aataagggca
     9901 gataaaagtt gctgcatctg caaaatgtcg cggcaatcgg caatcgatgc cgcacaatgt
     9961 ccgtggcgcg tcgctggccc catcaccacc gtgggcacgc cggtgccggt taaatgcacc
    10021 gctccgccgt ccgtgccgcc gttgctgaac atatccgcct gcaacggcac gccaatttct
    10081 gccgcgacgg tttcgatcca ggcggtaagt tttggcggcg caatcagcga cttgtcgctt
    10141 aacaccagca tcggcccgtt accaatctgg cgatggttag ccgcgctata atcaaagttt
    10201 ttcgcccagc aggcggtatc aagcacaatg gcgacatccg gcgacaccgc gcgggtggca
    10261 gtttgcccgc cgcgtaatcc cacctcttcg ctggaacttg ccaccagcca cacctccgca
    10321 ggcagttcgg cgtcgtgcag ctcacgtagt aacgtcacca gcagatagca accgaggcgg
    10381 tcatcaaagg ctttccccat cacccgctgg tgagggagaa cctgaaaagt tgtatcaaag
    10441 gtgacgcgat cgcctggacg aattcccgcc tgcatcactt cgtcatacga gcgcgcacca
    10501 atgtccacgc gcatggcgct gacgtcattc ccctgccggt cgccgtcaag caggcctgga
    10561 attttgcact cttcacgggt ggtgatgcgc accggctgca gctggcgggc agccatgcgc
    10621 acgttgccaa ccggcagcac gtcaatagcg ccttcgcggg agatgctgcg caccataaat
    10681 cccacttcgt ccatatgggc gcagatcatc accttcggac ctgtcgattc attgaggcgg
    10741 atcagcaccg atcccagacc atcaaatcgc acttcttttt gcaggcgatc cgcttcttcc
    10801 agtaggatct gccgcacttc ctgttccgag gaggcgatcg catctgcctc gctcaacgct
    10861 tttaatagcg ataaatccat tatgcctctc ccgtgagcaa cactgttttc ggcatggcgt
    10921 agagcacttc tgcgccttgc ggggtaacca gcacaacatc ttcgatgcgc acgccccctt
    10981 gccctggcaa ataaatcccc ggctccacgg tcagcaacat gcctggctgt agcgtcgtgg
    11041 tgtcccgcgg tgaaaaacgc ggatcttcat ggacttcaat gccgatagcg tgaccagtgt
    11101 tatgaccgaa atagtcgcca aatccagcct cggtaatgac ccggcgcgcg gcgtcgtcaa
    11161 tctgctggca gcgcacgccg gggcgaattg cagagattgc cgcgagctgt gcctgcagga
    11221 caatctgata gacgtcaaac agcgggtgag attcggcgct caccccttcg ccattcacca
    11281 gcaaggtgcg cgtcatatca gagcagtagc cctgatacag cgcaccaaaa tcgagagtga
    11341 caaactcgcc cgctgcaaca atcttgtcgc tggctttgcc gtgcggcagc gccccgcgcc
    11401 agccgctggc gacaatggtg tcaaaagagg ctttttctgc gccctgctgg cgcataaacc
    11461 actccagttc agcggctatc tcgcgctcgc tcatccccgc ctgaataaag cggcgaatat
    11521 gctccgctcc gcgatcggca atcccacagg cgaggcggat tatctccacc tcctctggcg
    11581 ttttgatttg ccgcagcaca tccggcgtgg cgctaaccag tttcgcattg agttcagact
    11641 tccagcggtg cgcggtttcc caactcacct gctggccttc aaaaccgagc gtttgcaact
    11701 gctcatcggc aatgatttga ttgacgatag tggtaagcgt gttcgtcgcg tcaagcaaat
    11761 gcagctggta gccttgcgca cgggcttcca cctcaacgaa atagcgcgaa tctaccagaa
    11821 tgtgcgcact ttcacggcta ataaccacat agcctgatcc ggtggagatc cccagatgcg
    11881 gctgtttgtt ctgccgtgag gagagaagca ctgcatccag ttgttgcgcc ttaagccagt
    11941 cgcgcagcga agcgagtaat gtcatctgga ttttccttct tacaggctat cgattaacaa
    12001 tttgccttta cggaacatca tcagacgcag gaacaccacc atcagcgcag ttatgactgc
    12061 ccccagcgcg atccccgcca tatagacgcc caggttagtg accagcggcc atgcccagat
    12121 agcggattcc gggaaccatt gcactgcgcc cagccagaca gcggcggttg agccgacaat
    12181 cgcgccgacc atatacgacg gaatggcggt gatggggctt tccagcgcaa acggaatcgc
    12241 accctcacta atccccataa aggcaaggaa catcgcggtc ttaccctgcg gataaagttg
    12301 agcgttgaac aggcgtttgc cggttaaacg acggtcaata atggtcgcca gacccagacc
    12361 aatcggagga ataacgatag cgatagaacg agcggtgacc ggtaatacgt ggtcggtggt
    12421 aaagctgaag gcaacgaaac ctgccgcttt gttgattggg ccaccaaggt cgattgcggt
    12481 cgccgcggca atccccattg catacatcag cgcgcccttc tcacctgcgg cagtcagcac
    12541 ggtacggata ccgccgttga tccagccacc aaatggggtg atgacatagt acatcgccag
    12601 catgacaaaa atggcggaaa gaatcggtag caaaaatgtg gttttgaacg ctaacaggaa
    12661 atctggtaac tggattttct ggttcatcca tttcacaagg taaccagcaa cgatagatat
    12721 aatcagcgca ccaatgaagg tggacggcac cggcgaagag gtcgcccatt gcatagtgct
    12781 gggatcgaag ttcagcagtt gcgtcggctg ggtagacatc aagccgccga taaaaccagc
    12841 cgggaacgcc agtttgccgc cgatagagtt cgccacaaaa gcggcaaaca tcggaatagc
    12901 aaagccaaac aacacgccgc cgaaggactg cgacagccag gcaaatttca gtaaggagag
    12961 gtcgaaaccg gagaatttcc cgctattaag agcatccatg atgccgatct cagcaggaat
    13021 tttaagccag ctataagcaa tcagctggct gaaagcgagg atcacaccgc ccataattaa
    13081 ggtcggcacc atgcgtgaaa tccctgacat cacatgctgc gggagttcac cccagaagct
    13141 gcttttagag gcctgcggat tcttaactgc cgctgccgca ccggatgcgc caggcacaac
    13201 ggttgcactg cgttttttaa tggccataac aattatccct gtcgaatatt actgttgttc
    13261 agaggcaatc atctcttcga tttctttgat gatgcctaca gcgtttttaa ttgcgtcctg
    13321 caaagtgatt tcataaacgt cgcgtgattc gaaacgttcg ttatcttccg gggtaactgc
    13381 cacggaatgg atgatgatgg tcgcttcggc gatatcctgc gccgtcaggc gattctggat
    13441 gccgtccgcg ccctgggttt caattttcac ttcataaccg gcttctaccg ccgcttcttc
    13501 cagcgcctga gcggccataa aagtgtgagc caggcccatc gggcaggcac ataaggcaat
    13561 cagtttctta ctcatcgtta attcctcgtt cagtgatagt tgacgagtga aattatgaac
    13621 gtgcaaaaca aatcgccgtt accagacaaa taatgcattt actggagaat taatcccgct
    13681 gtatggaagt gtgacgcagg tcggccttgt gaggggacac aaagaaaaat tgaattaact
    13741 gtatgattta aaagattatc gggagagtta cctcccgata taacaggaag gatttacaga
    13801 atgtgaccta aggtctggcg taaatgtgca ccggaaccga gaagacccgg attgtcatgg
    13861 acgatgagat acaccggaat atcatggaca tattctttaa agcgcccttt atcttcaaat
    13921 gcggcacgga aaccagaggc tttgaagaac tcaaggaagc gcggcacgat accgcccgca
    13981 ataaacacgc cgccaaatgt cccgagattg agcgccagat tgccgccaaa acggcccata
    14041 atgacgcaaa acagcgacaa cgcgcggcgg caatcggtgc agctatcagc cagcgcacgt
    14101 tcggtaatat cttttggctt gagattttct ggcaggcggt tgtcagcttt cacaattgcg
    14161 cgatacaaat tcaccagccc agggccagaa agcacgcgtt ccgccgaaac atggccaatt
    14221 tccgcacgca gtatttcgag gataatggcc tcttcttcac tattcggcgc aaaatcaacg
    14281 tgaccgcctt cgcccggcaa gcttacccaa cgcttatcga catggaccag atgcgcaacc
    14341 ccaagccccg ttccggcacc gtaaaccgca ataggcttac cttctaccgg ttctgcgcca
    14401 ccaaactgaa tcagatgctc ttttttcagc atcgggatcg ccatcgatac agcggtaaaa
    14461 tcgttaataa tttccagatg gctaaaaccg agattctttt tcatttcggc aattgagaac
    14521 gcccaggtat ggttggtcat tgccacccag tcaccggtaa ttgggcaagc gatggcaata
    14581 cagccgtctt tcacctcgac cttatgttct tcaagataaa cgcgaatgac cgcttcgagg
    14641 ctggggtaat caagccctga ataggtctta gcctgcgaga tttcaccact ggcaatatca
    14701 cacagagcaa gacgtgcgtt ggtgccgccc acatcaccga ctaatgcata ctttgtcatt
    14761 cttcaactgc tccgctaaag tcaaaataat tctttctcac actgtaaata cctgggggca
    14821 taacaacaac gccggaaagg caggctccct gtaaatatcg atctgggtca cacatttact
    14881 ttatcgtttc agcaccaatt gcagcgatgc ctttttgcaa gctgggcaaa ctaagtatct
    14941 gaccccgcat aaggaataga acatgctcca tccgcgagcc agaaccatgt tgttattatc
    15001 gctccccgcc gtggcaattg ggattgcgtc cagtcttatt ctgattgtgg tgatgaaaat
    15061 cgcctcggca ttacagaatt tgctctggca acgactgccg ggaactctgg ggatagccca
    15121 ggattcacct ctctggatca tcggtgtatt aacgctaacg ggtattgcgg tggggttggt
    15181 tatccgtttc agccagggtc atgccggacc agaccccgcc tgtgaaccgc tgatcggcgc
    15241 accagttccg ccctctgcgc taccaggact tatcgtagca ttaattctcg gtcttgctgg
    15301 cggcgtcagc ctggggccgg aacatccgat catgaccgtc aatatcgccc ttgcggttgc
    15361 cattggcgct cgtctgttac cgcgcgtcaa ccgaatggag tggactattt tagcctctgc
    15421 cggaaccatc ggcgcgctgt ttggtacacc tgttgcggcg gcgttgatat tttcgcaaac
    15481 cttaaatggc agtagtgaag ttccgctatg ggatcgtctt tttgcgccat taatggcggc
    15541 agcagctggt gcacttacta ccggattatt tttccatccc catttttcac tgcccattgc
    15601 gcattacgga cagatggaaa tgaccgatat tctcagcggt gcaattgtcg cggcgattgc
    15661 catcgcagca gggatggtcg ccgtatggtg cttaccacgg ttgcacgcga tgatgaatca
    15721 aatgaaaaat ccggtgctcg tgctgggtat tggcggattt attctcggta ttctgggggt
    15781 tattggtgga ccagtttcgc tgtttaaagg gctggatgag atgcagcaga tggtggcaaa
    15841 tcaggctttc agcaccagcg attacttttt gctggcggta attaaacttg ccgccctggt
    15901 cgttgctgcc gccagtggct ttcgcggtgg gcgaatcttc ccggcagtgt ttgtcggcgt
    15961 ggcattaggg ttgatgctgc atgagcacgt acccgccgta ccagcggcaa taaccgtttc
    16021 ttgcgctatt ctcggcatcg tgctggtggt aacacgcgat ggctggttaa gtctttttat
    16081 ggcggcggtc gttgtaccca ataccacatt gctaccgctg ctctgtatcg tcatgcttcc
    16141 ggcatggctg ttattagcag gtaagccgat gatgatggtc aatcgtccga agcaacagcc
    16201 accccacgat aacgtttagc aaaaaatgct ctcctttatg atttaagagt tatggcgatg
    16261 atacggggct ttacctcccc gtaatattgc gttaacaggc cgctgacggc ttcgatcgtg
    16321 aaggagaata acaatgttca ggtcactgtt tctggcggcc gccctgatgg catttacccc
    16381 gcttgcagca aacgcaggtg aaatcaccct actgccatca atcaaattac aaattggcga
    16441 tcgcgatcat tacggtaatt actgggacgg tggtcactgg cgcgaccgtg actactggca
    16501 tcgcaattat gagtggcgta aaaaccgctg gtggcgtcat gataatggct atcatcgtgg
    16561 ctgggataag cgtaaagcgt atgagcgtgg ctatcgtgaa ggctggcgcg atcgtgacga
    16621 tcatcgcgga aaaggccgcg gacatggaca ccgccattaa acacattcgc caatggaaca
    16681 caatgcctga tgcgacgcta gcgagtctta tcaggcctac aaaccggctc tttaacgcgt
    16741 agggcggata aggcgtttac gccgcatccg ccaatagtgc cagatgcgac gctcattcaa
    16801 ctacaatccc agcgccgtcc ccaccaacaa ccagatattc agcgccacga ccagcactac
    16861 aatcacccag cctgtctgtt ttacgcgttt gctgttcacc agatcgccca tcaacttgct
    16921 gtcactggtg aaaatcagca gtggaaccag cgccagggcg ataccaaaac ttaacagcac
    16981 ctgactcata accagaatcc gtgtcggatc taatcccatc agaatgacaa taaatgacgg
    17041 caacatggtg actgtacgac gcacccacag cgggatatgg aagcgaatga atccctgcat
    17101 caccacctgc cccgccagtg tccccaccac cgttgaggac agtccggcag caaccagact
    17161 taacccaaag accgttgccg cagcatggct taacagcggt tgcagcgtca gataagcctc
    17221 atcaagatcg gcaacaccag tatgaccaga aaagtggaac gccgccgcag ctgtagccat
    17281 catcgccaga ttgacaaaac cggcaatcgt catggcgata gccacatccc atttggtggc
    17341 ggaataacgt tgttgacgcg aaccgccatg taaatgctga gtgagcgaag agtgcaaata
    17401 aatcacatgc ggcataatcg tcgcccctaa cacgcctgct gccaggaaga ccgcttccga
    17461 agtaggtaaa ctcgggatca ccattccttt acccagctgc gccaggttag gctgggaaaa
    17521 aatcaactcg acaatgtaag ccgcggcaac aaacaacagt aacccaccaa tcactttttc
    17581 cagcggtttt tgcccgcgac gttgcagcat taaaatcagg aaagtcgcga tccccgtcag
    17641 caccgcgccc tgcaacaacg agacaccaag aatgagttta aaaccgatcg ccgcaccaat
    17701 aaattccgcc aggtcggttg ccatcgcaat aatttctgcc tgaacccaat agaaccacac
    17761 tacgggacgc ggatagtgat cgcgaatctg ctccgccaga tttttaccgg tggcaatccc
    17821 tagtttggca gagaggatct gaatcagcat cgccatcagg ttggcccaaa cgacaaccca
    17881 cagtagctga tagccgaagc tggcacccgc ctgaatattg gtcgcaaagt taccgggatc
    17941 gatataacca atcgccgcaa tgaacgcagg tcccattaat gcgagcctca tcttgcgcgc
    18001 cgcccgtccg ctgctactct caacgcgata gttcgtcatc ttgtgcctct aaaacatagc
    18061 ctttgctatg tttcatgcta tgccaaacga gaatgattat caaattcatt taaatgggtt
    18121 gtggtgattt ctctgataga cctggattat gactacgaaa agattgatga cttgaatgtt
    18181 atggttatgt ttaatgttag cacatttaca taactttcag cttccataca caacatagca
    18241 gaaatgtatg acagatcact atttttgaag cttgtcacag gacgtcatta tagtgtgtgt
    18301 cagatctcgt tttccttaac catgttacat agaatgtgca cggaaattta acctgcctca
    18361 tatttggagc aaatatggac cgcgtccttc attttgtact ggcacttgcc gttgttgcga
    18421 ttctcgcact gctggtaagc agcgaccgca aaaaaattcg tatccgttat gttattcaac
    18481 tgcttgttat cgaagtgtta ctggcgtggt tcttcctgaa ctccgacgtt ggtttaggct
    18541 tcgtgaaagg cttctccgaa atgttcgaaa aactgctcgg atttgccaac gaagggacta
    18601 acttcgtctt tggtagcatg aatgatcaag gcctggcatt cttcttcctg aaagtgctgt
    18661 gcccaatcgt ctttatctct gcactgatcg gtattctcca gcacattcgc gtgttgccgg
    18721 tgattatccg cgcaattggt ttcctgctct ccaaagtcaa cggcatgggc aaactggaat
    18781 cctttaacgc cgtcagctcc ctgattctgg gtcagtctga aaactttatt gcctataaag
    18841 atatcctcgg caaaatctcc cgcaatcgta tgtacaccat ggcagcaacg gcgatgtcca
    18901 ccgtatctat gtccatcgtt ggtgcataca tgaccatgct ggagccgaaa tatgtcgttg
    18961 cggcgctggt actgaacatg ttcagcacct ttatcgtgct gtcgctgatc aacccttacc
    19021 gtgttgatgc cagtgaagaa aacattcaga tgtccaacct gcacgaaggt cagagcttct
    19081 tcgaaatgct gggtgaatac attctggcag gtttcaaggt tgccattatc gttgccgcga
    19141 tgctgatcgg ctttatcgcc ctgatcgccg cactgaacgc actgtttgct actgtgactg
    19201 gctggtttgg ctacagcatc tccttccagg gcatcctggg ctacatcttc tatccgattg
    19261 catgggtgat gggtgttcct tccagtgaag cactgcaagt gggcagtatc atggcgacca
    19321 aactggtttc caacgagttc gttgcgatga tggatctgca gaaaattgct tccacgctct
    19381 ctccgcgtgc tgaaggcatc atctctgtgt tcctggtttc cttcgctaac ttctcttcaa
    19441 tcgggattat cgcaggtgca gttaaaggcc tgaatgaaga gcaaggtaac gtggtttctc
    19501 gcttcggtct gaaactggtt tacggctcta ccctggtgag tgtgctgtct gcgtcaatcg
    19561 cagcactggt gctgtaaaac catacataaa aaagccgggg atcattcccc ggctttttta
    19621 cgcctgttaa tcagctaatg gctgcgggcg accaatcaaa tacccttgca gatattgcac
    19681 cccgagctta tgcaatagcg cctgctgctg ttgcgtctcg acaaactccg cgaccacact
    19741 caatgacttc gctttcgcca gatcggtaat tgatctcaca atcatcgcat ccagcgtgtt
    19801 cgtgacaata tctttcacaa agacgccatc aattttgatg atatcagcct gcaaacgctt
    19861 taaccgttcg tagttggcat atccggtgcc aaagtcatca atcgcaatcc ggaaaccaaa
    19921 cttatgcagc tgctcgatgt tgtacatgct gctttctgcg ttagaaaacg cctgctcctc
    19981 ggtgatctca agaatgaccg actgcgggga gatgtgataa cgcttaaaca gacgaataat
    20041 ccgcccggca atattctttt gcagcagcgt gagcggcatt aaattgactg aaaagcgcgg
    20101 tccttttttg tcgcaagggt gtgttgccag ccacttcaac aaggattcca gcacttgcaa
    20161 atcaaaacgc gcgctaaggt taaactgagc aataaggggc agaaacttat ccggggtcat
    20221 aatgccgccg tcatatttca gtcgcgcgag gatctcatca taaccttcac cctctttgtt
    20281 gcgaattggc tgggcgtaga gcagcaaatc accctgatct aacgccgtgc gaatggtatt
    20341 cagcaatagc acctgtttag tggtctgccc ggaaaccatc tcctctctgc tatccagcgc
    20401 cagcacatga tgatgtgcgc aggattgctc cgccagccag cttaactgcc ccaacaaggg
    20461 ttgcagggtt tcctgatttc catcaaaacg cccccaggca gcaccatagc ccatatccag
    20521 cccggtattg ttccagtgaa tttgccgact attcaggatg ttaaccatat gctggagtcg
    20581 cccttccgtt tccggcccgc tcagcactaa cagcagttca ctacccggca attgatacaa
    20641 cttttcgttt tcctgcatca acggcagcag cgtacggcaa attgagcgga tacagtgaac
    20701 gcgcatcatt aagccgtaat gacgactcat aaactcaaga ttatcaatgc gcaggcagca
    20761 aaaactcttg cccgcctctt gttccggcgc ttgttccaac gcacgaaagt tgggtaggag
    20821 cgttaacgga tctgtcagcg cctgcaaatg ccagcggcga ttaagccatt cactgcgatg
    20881 ataaatgcgc accatataga gcaggcaaac gctaaaggaa atcagcaccg caagaataaa
    20941 tgccagcgaa tattcggttt ccaccccttg caaaaagttc tggttgtaat tcagcaggca
    21001 aagcgttgaa acagcccagg tgagatttaa aaacggatag cgaagcttac cgaccccgag
    21061 ggtaaagatg atgaagaaaa cgggtaccaa gtaaccggca ataaagtcgt tttcataagg
    21121 tgtgcacaac agaagtagca gcacgcttaa cgctgccagc cagcttaagg taaatgcgcg
    21181 tttctctttg cccaacgacg gagcgatatc cctgcgccac aatatctgcg caaagtgggg
    21241 acttacaatc atgcgagtga gatagtagaa aagcatgttg taaatcagca cagcggtgaa
    21301 aaggcttagc aaatcaacga ccgtgaaaat ggcatccgca tcgccgaaaa aggtggatat
    21361 ctttagcggg aaatcaaaga agctccccac aagatacata ctgcatttga tgccgatcgg
    21421 cgtcaccaaa ccaagccaga acagacgttg ccagacatat cggctggtca atccataacg
    21481 ccaacgcgtc cccagctgcc agcgtaatat tgcgcaggca cacaacacga caaacgtctg
    21541 gctaccgagc atgaccgcag tctgtaaaaa agagagatga aagttccaca gattggtgca
    21601 aaacatcccg cataaaacgg gaacgacacc gcgccagcca aagataaaca agattgacag
    21661 cattacgcac agtggcatcc acgccagaaa gatataactg gaatggataa tggcaagtgg
    21721 cgaaataaaa cgggatagct gaataagtac cacggtgagc gtaaacgcta gtgtgaatat
    21781 cttgatattt tttatcaggt tatgctccac aaacatgagt acactactct cactgatcgg
    21841 ggttttcagg cacacaaata ataagtttat tcgcatcgcg aagcaaacag gggccagcct
    21901 gttgccgatt tttcatccaa tgatgctttc accaggcggc gctaacccgg acgtagactg
    21961 tattacaaaa gcggcaaaaa gcagagacaa aaaacccccg ctttgcagcg agggttggaa
    22021 atttggtgga gctaagcggg atcgaaccgc tgacctcttg catgccatgc aagcgctctc
    22081 ccagctgagc tatagcccca cgatgcgttt acgtaccaaa tttgctgggt gcaaaatttg
    22141 gtggagctaa gcgggatcga accgctgacc tcttgcatgc catgcaagcg ctctcccagc
    22201 tgagctatag ccccgtcacg taaagcttgt cgagttgacg ggcggcatca tatgaattcc
    22261 gcccgaatgt gtcaacggca aattgcaacg tgtagtttca atcgctgaaa aatcaggcaa
    22321 atgaacaatt ttggaaaacc gctcgcattc agtagttatt catgtcacgg tttcctgtaa
    22381 agtggtgtta taaaatgaac tactaataga cccacataca ttcagggaat tgttatgttc
    22441 aaggagagga tgacaccaga tgaacttgcc agactgaccg gctatagccg ccagaccatt
    22501 aataaatggg tacgcaagga aggctggaca acgtcaccaa aacctggcgt ccagggtggc
    22561 aaagcccgac tggttcacgt caatgaacag gttcgtgaat atattcgcaa tgctgaacgt
    22621 ccagaaggtc agggagaagc gcctgccctt tctggtgatg cgccgcttga agtattgctg
    22681 gtgacactgg cgaaagagat gacgccagtt gaacaaaaac agtttacatc cttgcttctg
    22741 cgggaaggga ttatcggatt gttacaacgc ttagggattc gcgatagcaa ataatatgaa
    22801 aagattacgc aacaaaatga ccaccgaaga actggctgaa tgcctcggtg tggctaaaca
    22861 aactgttaac cgttggatca gagaaaaagg ctggaaaacg gaaaaatttc ccggcgtgaa
    22921 aggcggtcgt gccagactta ttctggtcga tacgcaagtt tgcgagttta ttcagaacac
    22981 gccagccttc cataacacac caatgttgat ggaagctgaa gagagaattg ctgaatacgc
    23041 tcctggagca cgcgccccgg cgtatcgaca aatcatcaac gcgatagata atatgacaga
    23101 cattgagcag gaaaaagtcg cgcaattttt gtcacgtgaa ggaattcgca atttcctcgc
    23161 ccgtctcgat attgacgaat cagcataaat aaaaaacggc aggatattat ctcctgccgt
    23221 ttatcttttt acacgctaat tactgctgat tttcgcgttc agcaataaaa tccagcgctt
    23281 tgttgatacg ctcgatactg cgggtcttac caattgcgtg aacggtaaca tccagtgctg
    23341 gagactgccc cgcaccggtt acggcgacac gcagcggcat accaacttta cccataccca
    23401 cttccagctc atccgccgtc gcctgaatag cgtgatgaac gttttcagcg gtccagtcag
    23461 taatcgcggc cagtttgtca cgaaccactt ccagcggctg acgcgctacc ggacgcagat
    23521 gttttttcgc cgcatcggca tcgaactcag caaaatcttc gtagaaataa cggcagctct
    23581 gtgccatctc tttcagcgtc ttgcagcgtt cgcccagcag tttcaccaga tcagccagct
    23641 gcgggccgtt acgggtatcg atattttcct gctcaatgtg ccactgtaag tgagtagcaa
    23701 catactccgg cggcagcgcg ttaatgtagt gatggttcag ccacagcagc ttgtcggtgt
    23761 tgaacgcact ggcagatttg ctgacggcat tcaaagtgaa gtatttgatc atctcttcac
    23821 gagtgaagat ttcctgatcg ccatgggacc agcccagacg caccagatag ttcagcagtg
    23881 cttctggcaa ataaccgtca tcacgatact gcattacgct gactgccccg tgacgtttgg
    23941 acagtttttt accgtcatcg ccattgatca tagaaacgtg cgcgtaaacc ggcaccggcg
    24001 ctttcagggc cttaagaatg ttgatctggc gcggcgtgtt gttgatatgg tcttcgccac
    24061 ggataacgtg ggtgatttcc atatcccagt catcgacaac cacacagaag ttataggttg
    24121 gggaaccatc ggtacggcgg ataatcagat catccagctc ctggttgctg aactcgatcg
    24181 gaccacggat ctgatcgtca aaaacaacag aaccttcctg cggattagca aaacgcacga
    24241 cacacggttc atcatcagca tgatgctcat ggctgtggcg gcagcgaccg tcataacgcg
    24301 gcttctcacc tttcgccatt tgctcttcgc gcagcgcttc caggcgctct ttagagcaat
    24361 agcatttata agcagtgccc tcttccagca tctgatcgat caccgcattg tagcgatcaa
    24421 aacgtttggt ctggtagtac ggaccttcat cccactccag gctcagccag ttcatgccat
    24481 ccataatggc ttcgatagct tccggcgtgg aacgctcaag atcggtgtct tcaatacgca
    24541 gcacgaactc accgccgtgg ttacgtgcaa aaagccagga gtaaagagca gtacgcgcgc
    24601 cgccaacgtg cagatagcct gttgggcttg gcgcgaagcg agttttgatt ttcatgaaat
    24661 ggccttacgt ttagaaagat gccgacaacc ggcaaatcct ggaaaaattg agtgggcgat
    24721 attctatcac tcccgcctga ttcctcaatg tagttcgggt ttctacctta cgcattattg
    24781 ttttcgttga caaattgcgc aacacggtct gttttgcgat catcttgctt aaatttacga
    24841 cgaacgaaca atttctttag aaaatgcgtt gactcatttt gaactctccc tataatgcga
    24901 ctccacacag cgggggtgat tagctcagct gggagagcac ctcccttaca aggagggggt
    24961 cggcggttcg atcccgtcat cacccaccaa ctactttatg tagtctc
//
LOCUS       DAZGVQ010000069        24810 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000069 DAZGVQ010000000
VERSION     DAZGVQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24810
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.069"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1555)
                     /gene="ycjM"
                     /locus_tag="ACHLAG_002661"
     CDS             complement(<1..1555)
                     /gene="ycjM"
                     /locus_tag="ACHLAG_002661"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFP0655863.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGKSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLF"
     gene            complement(1768..2082)
                     /gene="pspE"
                     /locus_tag="ACHLAG_002662"
     CDS             complement(1768..2082)
                     /gene="pspE"
                     /locus_tag="ACHLAG_002662"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFP0655864.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCSAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(2157..2378)
                     /gene="pspD"
                     /locus_tag="ACHLAG_002663"
     CDS             complement(2157..2378)
                     /gene="pspD"
                     /locus_tag="ACHLAG_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFP0655865.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(2387..2746)
                     /gene="pspC"
                     /locus_tag="ACHLAG_002664"
     CDS             complement(2387..2746)
                     /gene="pspC"
                     /locus_tag="ACHLAG_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFP0655866.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2746..2970)
                     /gene="pspB"
                     /locus_tag="ACHLAG_002665"
     CDS             complement(2746..2970)
                     /gene="pspB"
                     /locus_tag="ACHLAG_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFP0655867.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(3024..3692)
                     /gene="pspA"
                     /locus_tag="ACHLAG_002666"
     CDS             complement(3024..3692)
                     /gene="pspA"
                     /locus_tag="ACHLAG_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFP0655868.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3859..4836
                     /gene="pspF"
                     /locus_tag="ACHLAG_002667"
     CDS             3859..4836
                     /gene="pspF"
                     /locus_tag="ACHLAG_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFP0655869.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4956..6221)
                     /gene="puuE"
                     /locus_tag="ACHLAG_002668"
     CDS             complement(4956..6221)
                     /gene="puuE"
                     /locus_tag="ACHLAG_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFP0655870.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGKPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDVQFDAAMKILQDALSD"
     gene            complement(6259..7539)
                     /gene="puuB"
                     /locus_tag="ACHLAG_002669"
     CDS             complement(6259..7539)
                     /gene="puuB"
                     /locus_tag="ACHLAG_002669"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFP0655871.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7541..9028)
                     /gene="puuC"
                     /locus_tag="ACHLAG_002670"
     CDS             complement(7541..9028)
                     /gene="puuC"
                     /locus_tag="ACHLAG_002670"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFP0655872.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANLV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(9303..9860)
                     /gene="puuR"
                     /locus_tag="ACHLAG_002671"
     CDS             complement(9303..9860)
                     /gene="puuR"
                     /locus_tag="ACHLAG_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFP0655873.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9887..10651)
                     /gene="puuD"
                     /locus_tag="ACHLAG_002672"
     CDS             complement(9887..10651)
                     /gene="puuD"
                     /locus_tag="ACHLAG_002672"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFP0655874.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            10863..12281
                     /gene="puuA"
                     /locus_tag="ACHLAG_002673"
     CDS             10863..12281
                     /gene="puuA"
                     /locus_tag="ACHLAG_002673"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFP0655875.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDAEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDAEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            12430..12594
                     /locus_tag="ACHLAG_002674"
     CDS             12430..12594
                     /locus_tag="ACHLAG_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655876.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRLTRPQTL
                     LRRGGSNYGY"
     gene            12584..13969
                     /gene="puuP"
                     /locus_tag="ACHLAG_002675"
     CDS             12584..13969
                     /gene="puuP"
                     /locus_tag="ACHLAG_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFP0655877.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVAMSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKEHFHYLLMPLVGALTVGVLWINLEATSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            14103..14348
                     /gene="ymjA"
                     /locus_tag="ACHLAG_002676"
     CDS             14103..14348
                     /gene="ymjA"
                     /locus_tag="ACHLAG_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HFP0655878.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14661..16304
                     /gene="sapA"
                     /locus_tag="ACHLAG_002677"
     CDS             14661..16304
                     /gene="sapA"
                     /locus_tag="ACHLAG_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFP0655879.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            16301..17266
                     /gene="sapB"
                     /locus_tag="ACHLAG_002678"
     CDS             16301..17266
                     /gene="sapB"
                     /locus_tag="ACHLAG_002678"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFP0655880.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            17253..18143
                     /gene="sapC"
                     /locus_tag="ACHLAG_002679"
     CDS             17253..18143
                     /gene="sapC"
                     /locus_tag="ACHLAG_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFP0655881.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            18143..19135
                     /gene="sapD"
                     /locus_tag="ACHLAG_002680"
     CDS             18143..19135
                     /gene="sapD"
                     /locus_tag="ACHLAG_002680"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFP0655882.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            19137..19943
                     /gene="sapF"
                     /locus_tag="ACHLAG_002681"
     CDS             19137..19943
                     /gene="sapF"
                     /locus_tag="ACHLAG_002681"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFP0655883.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            20011..20364
                     /gene="ycjD"
                     /locus_tag="ACHLAG_002682"
     CDS             20011..20364
                     /gene="ycjD"
                     /locus_tag="ACHLAG_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFP0655884.1"
                     /translation="MMDKIKSNARDLRSNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSS"
     gene            20732..21520
                     /gene="fabI"
                     /locus_tag="ACHLAG_002683"
     CDS             20732..21520
                     /gene="fabI"
                     /locus_tag="ACHLAG_002683"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFP0655885.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21665..22792
                     /gene="yciW"
                     /locus_tag="ACHLAG_002684"
     CDS             21665..22792
                     /gene="yciW"
                     /locus_tag="ACHLAG_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFP0655886.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22860..24794
                     /gene="rnb"
                     /locus_tag="ACHLAG_002685"
     CDS             22860..24794
                     /gene="rnb"
                     /locus_tag="ACHLAG_002685"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFP0655887.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
BASE COUNT         5657 a         6534 c         6429 g         6190 t
ORIGIN      
        1 taaacaatcc agtcagacaa ttaccatcag cgttacttct ttgaatacgc attacggatg
       61 aattaatcgt atcgatggta aaattattat ccggatgaaa ctcgttatgg ctgcgacgaa
      121 gtgtaattaa acgcgacaac tcatgatata ccgcatgcct taatgtagct tcatcgttca
      181 gttctcgggt tatctcttta ctgtgatatt ttttacggtt aatcgcacgg ttatatccga
      241 gtttttcgac acctgcgtaa tcattacgcg agccaagaat actttgaata tatatcgctg
      301 gcacaccggg gaaacttaac aaaatcgcat gggcgagaat aaacctggcg caacgttctt
      361 catcgctact ctcacggcgg cttaacgcat ccatataggt cacatttatt tcatacggac
      421 tgcgtgtacc gtcgggatta tttttccagt ttactaatgc accttcctgc tgtaacgcct
      481 cgaccagctc taatatttcg ctttcaggca acaagccccg tagcgggttt agcccgatgc
      541 catcgtgaga ggcgaggaag ttaaaccagg tggtgttgct ggaaggtagt gtcaggtttt
      601 gcgcccacgc acaaagcgcc tcaacgttct gtttttgcac cgcatgcagc accagtggcg
      661 gcagcgagaa ctggtacacc atatgtgctt cgtcatcgcc tgcgccaaag taagcaatgt
      721 tgtctttatg cggaacattg gtctcggtaa tgatcactgt acctggcgca acgttatcaa
      781 taatcgaccg taacagtttg ataatcagat gtgttttttc cagatggatg cagctttttc
      841 ccggctcttt ccacataaag ccaacggcat ccaggcggac atactcagca cctttcgcaa
      901 ggtaacagag taaaacatcc accatcgcca gcaacacttc agggctacgg taattcaggt
      961 caatttgatc gtcactaaag gtggtccata aatggcgcgt tgaatgatcg cgcatctgga
     1021 atggcgttaa taacggtaac gcacgcgggc gagtgacggc gctgagatcg gtttgcgggt
     1081 caacggcaat aaaaaaatct tcaaaacctg gatgctgttg taaatagttt ttaaaccatt
     1141 cactttttgc cgacatatgg ttgcagacaa aatcaaacat taaatgactg cattcaccga
     1201 gttgctgaat atcctgccac tcccccgctt cactggcgac ctgatgataa tcaattaccg
     1261 aaaagccatc atcagatgac catggataaa acggcaacaa atgaacatgt gaaaaaatgc
     1321 tttgcagcca ttgatggtaa aactgattaa atgtgggtaa tggttttaaa tcattgctgt
     1381 gaaattgatc ggcataggta attaacacga catcactttc atcccagtgt tttttacgtc
     1441 gctgtgtaat taatcgtttc gcactctcaa gacgcgttac aagtttctgt aaatgagttg
     1501 cggtaaatgt tccaccgtag atttcgtcca ggtaatccgt aattttctgt ttcatgttgc
     1561 ctttccgtgg tagcggtccc atgaaacgag agactactcc gcagaaaata aactgtcaat
     1621 tgagcacaag ggttttagtc acgcaggaaa tcatttttta ccagggaaaa agcgtatttg
     1681 tgagctgacg caaataatct atggggatgt aaataaggtg tgacagaaaa aaaaacggcg
     1741 cataagcgcc gctcatggtg aattctttta acctttgacc ttcggcattg cgatgtcttt
     1801 caggccaccg gcgttctcaa cgtgggtata tcccatctcg ctaaggatct cttttgcttg
     1861 ccctgactgg cgtccggcac tgcaatacac tttcacggtg tcgtttttat ccggaacggc
     1921 ggtggcaatg cgctctttca cttccttcag gggaatattg atggccccct gaacgtgctc
     1981 ttgctgatac tgctctggaa cacgaacatc gatccagtgt tcagcggcga aaacgggcag
     2041 tgaaaacacc agtgccagag caagtaagcc ttttttaaac atgtaagcgt ccttctatgg
     2101 atgaaaattg ccatccatta tacgcctgag atttaacaat ttttgcgaaa ctcccctcac
     2161 cttttataac gctgtgccag tttattagca gcccgactta acagcggttc cagcgccaca
     2221 gccagcaaca ttttcagcgg tcggcgagca actgatttta tcgcccagcc cgctacaccc
     2281 gccgggccat agcgcagtgc ggtaagaagt accagcttgc ctgctaattt gaaaccaggc
     2341 tttacctttt gcccggcctg ttgccagcga gtattcataa ctttcctcac agttgacgga
     2401 aacggctacg taacgtgaaa gtatcggaag tgacataacg ttccatctcg cgtaaacgcg
     2461 tttcacttgc cgccagttca cggtcgactt catccagcaa ttcgctgctg gaaggtagct
     2521 gctcaccaaa ggccatgttg tccggcattg gatcaagcgc aaatgacaaa atgatgtaag
     2581 caaccagggt aaacagcgcc agaccgaaga aaatcgacag caccaccagg atacgcacca
     2641 gttttgccgg tacatcaaaa tagttggcaa tcccggcgca gacgccgcgg accatgccct
     2701 gctgtggaat acgccataat tttttattga gattaatgcc cgccattagc gatccctcca
     2761 gttcggatgt tctgcatcaa gaatagattc cagcgcctga atacgttcgc gcatccgttt
     2821 tgcttcatca gccagttgcg ctaatcgctg ctgctcactt tgcgacaatt cactgcgacc
     2881 agaacgattg ctgtaatgca gccataacca gatcggtaaa acaaacagca caaaaatggt
     2941 taacggaata gccagaaata gcgcgctcat aagtactcct tagacgatga gcggcggcgc
     3001 gttcggacgc cgcctggatg ttattattga ttgtcttgct tcattttggc ttttaattgt
     3061 gccagttgtt cgctgattgc atcatcggct ttcagttcgg caaactgatc gtccagcgat
     3121 ttttgtttac cgaagctgtg gctttctgct tccgcttcca tctggtcaat acgacgttcg
     3181 aaagattcga aacgagccat tgcttcatcc agtttgccac tgtccagctg acgacgcaca
     3241 tcgcgcgacg agtttgccgc ctgatgacgt aacatcaatg cctgctggcg agcgcgtgtt
     3301 tcgctcaatt tgttttccag ctcaccgatc tctttcttca tgcgtgccag cgtgtcgtcc
     3361 accagcgtca cttcatgttc caggctctta atcagatcgg tcagtttctg tttttcaatt
     3421 aacgctgcac gtgccagatc ctctctctct ttcagcagcg ccagttcggc tttttcctgc
     3481 cattcaactt cacgcgccga cgcttgttca atacggcgag tcagctgttt cttttctgcc
     3541 aacgcgcgcg ccgaagtaga acgtacttca accagtgtat cttccatctc ctggatcatc
     3601 agacgaacca gtttctgtgg atcttccgct ttctctaaca gagcgttgat gttggcattc
     3661 acgatgtcgg caaagcgaga aaaaataccc ataatgttgt cctcttgatt tctgcgttca
     3721 ggattgtcct gctgaactgt taatacaatt tgcgtgccaa ttttttatct ttttgattta
     3781 taaagatctg attgaagaat caacagcaac atgccaggat gagttagcga attacactaa
     3841 caagtggcga atttcatcat ggcagaatac aaagataatt tacttggtga ggcgaacagc
     3901 tttctcgaag tgctggaaca ggtttcgcat ctcgcaccgc tggacaaacc ggtgctcatc
     3961 atcggcgaac gcggcaccgg taaagagctg attgccagcc gcctgcatta tctctcctcc
     4021 cgttggcaag ggccgtttat ttcccttaac tgcgcggcgt taaatgaaaa tctgctggat
     4081 tccgaactgt ttggtcacga agcgggggcg tttaccggtg cgcaaaaacg tcatcctggg
     4141 agatttgaac gtgccgacgg cggtacgcta tttcttgatg aactcgctac ggcaccgatg
     4201 atggtgcagg agaaattatt gcgcgtgatt gagtacggtg aactggagcg cgttggcggc
     4261 agccagccat tgcaggtgaa tgtgcggttg gtatgcgcga cgaatgccga tctcccggcg
     4321 atggtcaatg aaggcacttt tcgcgctgac ctgctcgacc gactggcttt tgatgttgta
     4381 caactgccac cactgcgcga gcgcgaaagc gacataatgt tgatggcaga acactttgcc
     4441 atccagatgt gtcgggaaat caagctgcct ctgttcccgg ggtttacgga gcgcgccaga
     4501 gaaacattgc tgaattatcg ttggccggga aatattcgtg aattgaaaaa cgtggtggaa
     4561 cgttcagtgt atcgccacgg caccagcgat tatccgcttg atgacatcat tattgatccc
     4621 tttaaacggc gtccgcctga agacgctatc gccgtttcag aaaccacctc gcttccaaca
     4681 ctgccgctgg atttacgtga gtttcagatg cagcaggaaa aagagttgct gcaactcagt
     4741 ttgcaacaag ggaaatataa ccagaagcgc gcggctgaat tactggggtt aacttatcat
     4801 cagttccgcg cgttgttgaa aaagcaccag atttagcgaa gttgagcaat acaacttgca
     4861 ggatgcggcg tgaatgcttt attcggcata cataatcaat acgtcgtttg caggcctgat
     4921 aagcgcagcg catcagacat tattgcgttg ggctattaat cgctcagcgc atcctgcaaa
     4981 attttcattg ccgcatcgaa ttgcacatcc gggatggtca gcggatacag gaagcgaatc
     5041 acgttgccgt atgcgccaca ggtcagcagg agcagcccct gcgccagcgc gcgttgctgg
     5101 attttctgtg caatcgccgc tgacggcttg cccgtttgcg gatcgttaaa ctctgccgca
     5161 atcatcgacc ccaggccgcg taccgcagca atggccggaa cgctttcttt ggcatcaatc
     5221 aacgtgtttt tgagacgctg gcccagttga ttcgcgcgtt cgcagagtga ttctttgtcg
     5281 ataatattga gcaccgcgtg cgcggcagcc accgccagcg ggttcccggc gtaggtgcca
     5341 cccaacccgc ccggcgcggg cgcgtccata atattcgcgt taccgaccac gcccgaaagc
     5401 ggcatcccgc ccgcgaggct tttcgccatc gtcattaagt ccggcttatc ggcgtaatga
     5461 tccatggcaa acagtttacc ggtacgcgca aagccgcttt gcacttcatc ggcaatcatc
     5521 acaataccgt gctcgtcgca caggcggcga atggcggcaa ccagctcttt tggcgcaacg
     5581 ttgaaaccgc cctcgccctg caccggttcg aaaataatgg ccgcgacctg cttcgcttcg
     5641 atgtctgatt taaacaagcg ttcgatggcg tcgagggagt cctgtgttga aatgccgtgt
     5701 aaatctgacg gataaggtac gtgatacacc gaaccaggga acgggccgaa gccgattttg
     5761 tacggcgcaa cttttccggt cagcgccatg gtcatatatg tacgaccgtg gaagccaccg
     5821 ctaaacgcaa tcacgccagg tcgtccggta tgggcgcgag caattttcac cgcgttttcc
     5881 accgcttccg caccggtagt gaagaatgcg gttttagcct gcccgctcac tggggcaagg
     5941 gcgttaattt tctccgccag cgtgacgtag ctttcgtacg gcacaatctg atacgccgtg
     6001 tgggtaaact gttgcaattg ctgctccacc gccgcgacca gatcagggtg gcgatgcccg
     6061 gtattcagca ccgcaatgcc tgcggcgaaa tcgatgtatt cgttgccctc tacgtccttc
     6121 agcgtagcgt tttctgccga ctgggcgaag aagttacaca tcacgccaac cccgcgtggg
     6181 gtagcagaaa gacgacgctg atggaattca ttgttgctca tgtttcacac cttttgactc
     6241 ttcactgtta atcggaaatt aaacgcccag acgatcgcgc aggctgtaat aagccgcgcc
     6301 catcgcggta aatggcacgc gcagcgtgcg cccgccgggg aacgggtaat gcggcagatt
     6361 ggcgaaggca tcgaaacgtt cggcgtcgcc gcgcagcagt tcggcaatca gacgcccggc
     6421 aagatgggta caggtgacgc cgtggccgct gtagccctgc atgtaataga tgttggtatc
     6481 aaggcgaccg aactgcggca tgcgcgacaa ggtcagcagg aagttgcccg tccagcggta
     6541 atcaattttc acgcccttca gctgcgggaa ggttttcagc agtttcggca ccacaaggcg
     6601 ctcaacgtca tccgggtcgc gcgcgccgta gaccacgccg ccgccgtaca gcaggcggtt
     6661 gtcggcggta agacggtagt aatcaagcag atagttacag tcttccacac agtagttttt
     6721 cgggatcagc gaacgggcta aatcttccga cagccgttcg gtggtgatca cctgcgtgcc
     6781 gcacggcatg ctgcgtttcg ccagttccgg ctctacttta tcgcccagat acgcattccc
     6841 ggcgacaatc acatacttcg ccgtcacctg acctttggca gttcgcacaa cggctggcgt
     6901 ggtgtgctgg atctgcgtca cggcagaaag ttcatacacg cgcccgccgt tgaggcggat
     6961 ggcgtccgct tcgccaatgg cgaggtttag cggatgaata tgcccaccgc tgtgatccag
     7021 cagcgcaccg gtatagcgat cgctggctac ttcacggcga atggcgttgg cgtccagcaa
     7081 ttccagctgt ttattgccgt agcgttccca gttctctttc tgctcttcaa gtgtggcgag
     7141 ctgtttatca ttcatcgcca caaacaggcc gccggggcgg tagtcgcagt caatctgata
     7201 acgtttgata cgttcgcgga tgatctcacc accttcgaac atcatgctgc cgagcatacg
     7261 ggcggtgtcc atgccgtagc ttttttcgat cacgtcgatg tcgcggctgt aggagttcac
     7321 aagctgcccg ccattgcgcc cgcttgcgcc aaagccgatg cgtgaggctt cgagaaccac
     7381 tacgtcaaag cccgcttccg ccagatgcag cgcggaagag agcccggtat agccgccgcc
     7441 gaccacgcaa acgtcgcagg aaatcgactc attcagcgtg tcgaatggcg cgtatttatt
     7501 cgcactggcg gcgtagtaac tgctggtatg ttcggtcatt tcaggcctcc aggcttatcc
     7561 agatggtttt cagttcggtg aatttttcaa gggcatgcag ggatttgtcg cgaccgttgc
     7621 cgctctgctt atagccgcca aacggcacgg tcatatcgcc gtcgttgtag ttattgacga
     7681 agacggaacc agccttcagg cggcggctca tgcggtgcgc gcgggagagg tcgcgcgtcc
     7741 ataccgccgc gccaaggccg tactggctgt cgttggcaag ctgtagcgcc tggtcttctg
     7801 atgtgaaacg cgtgaccacc agcaccggac cgaaaatctc ttcgcggctt aaggacgcat
     7861 tcgggtctac atccacaaag atggtcgggc cgatggcggc agccagctca gcgttacgac
     7921 catccagcag cagttgccct ttgctttcgc cttcccgaat aaagctatgg accgagtcgg
     7981 cgtgggcgca gtcgattaag gtgcccatgg tggttgcggg atcaagtgga tggcccggct
     8041 gccagttttg cgcctgctgt tttaacaggg ctaagaattc atcggcgatg ctctcttcca
     8101 gcaacaaacg cgttccggcg atgcacacct ggccctggtt gtagaagatg cctgcggcgg
     8161 tggcgcttgc cgctttttgc agatccggac aatcggcgaa aacgaggttg gcgcttttgc
     8221 cgcccgcttc cagccagacg cgtttcatgt tgctgtcgcc cgcatctttc agcagctgtt
     8281 tcccggtacg ggttgaaccg gtaaaggcaa tggcgtcgat atcgttatga cgcgacagcg
     8341 cctgccccgc ttcatgacca aaacccgtca ccacgttcaa cacaccatcc ggcaagcctg
     8401 cttctttcgc cagccccgcg agacgaatcg cactgagcgg tgatttttca gacggtttta
     8461 gaatcacgct gtttcccgcc gccagcgccg ggccgagttt ccagcaagtc agcaacagcg
     8521 ggaagttcca cggcacgatg gcggcaatca cgccgaccgg ttcacgcacg atcatcgcca
     8581 gctcatggct actggtggtc gccacttcgc catacacttt gtcgatcgct tcggcgtacc
     8641 agcgaatggc gcgcgccgcg ccgggaatat catcacgcag actgtgacga atcggtttgc
     8701 cggtgtcgag agtttccagc agtgccagct cttcggcgtg ggcttccatt aaatcggcga
     8761 gtttattcag taccgcttta cgtttagccg gagaagacag tgaccagtcg ccgcgttcaa
     8821 atacgccgcg tgctgcgctc accgcacggt cgatatcgac gctcttgccg cgggcaattt
     8881 tcgccagcgg tgcctgggtg accggatcaa cggtttcaaa ggtttcattt tccgccgcag
     8941 cagtatattc accgttaata aataagcggt tttcaatggc gagacttaac gctttatcct
     9001 gccagtaagc cagatgatga aaattcatta tgactcctgt ttcacgtcta tcagatatat
     9061 gcagtttgtg aacgtcaggg cgcagagatc cccgggccgt atgcccgttt atttctggcg
     9121 acattcttta tatacacttc atccttcaag ccgcctctgc cttggctgcg ttcgctcccc
     9181 cgaatcactt acctgagtaa gctcatcggg attcgctcac ttgccgcctt gatgcaactt
     9241 gaatgatttt gtgtatagat tgccgggcgc attgcgctta cccggcttac agaacaaaaa
     9301 gattaaaacg tggtgggcgt atgggcgctg ataattcggc aaatacctgc cgacgtatta
     9361 ctgaaactgt gcggtatgcc ggtattaatg gcataacttt gccccgcgac gaggtggtaa
     9421 tcctgaccat taatcgtcag aacaatttca ccttccagta ctgtgcctat ttcctcaccc
     9481 tgatgcttaa ttctttcccc agtggttgtg cccggctggt acgtttcaaa gatcatcgcc
     9541 agcgtgcgat tcgggttacc gttatgaacc agcttcattg acacaccctg actgcccatc
     9601 tcaattaagt cgtcctgatt aatgacgacc tgcggctcat caggtttttc cggctcggaa
     9661 aagaattccg agagtgacag accatacacc ttcagcagct tttgcagcgt actgatggca
     9721 gggctgactt tatcttgttc tatcgtactg atagcactgt gagtcagccc ggagagttcg
     9781 gcggcacgac gttgtgaaag cccctgctgc tggcggattt ccgacaagcg ttttcctggc
     9841 gccagtccct catcactcat atttgcattt ccttaaactg tagtggtcag agtcgctgtt
     9901 tttcagcgat atggtgctga caagcggtga taaagccctc gaacaatata cgcgaaagcg
     9961 cgtactcgct actgttccat tccgggtgcc actgtacgcc cagcgcaaaa ggatgattga
    10021 tgacgctaac cgcctcaact aagccatccg gcgagcgagc ttcaacacgc aaccgtgggc
    10081 taacgacctt cgccccttgc ccatgcagag agtttaccca aaagttgcta cattcaggta
    10141 acaacgcaga cagtaatccc ccctcttcaa cctgaacttc atgcgacggt gcatattgct
    10201 gttccaccgg cagttcggga tcttcccgat gttccagcaa ttcaggctgt tcgcacagct
    10261 tgcgatgcaa cgaaccaccg gttgccacca ccagttcctg taaaccccgg cagatggcga
    10321 aaatggggat gcgcctttcg agtgcggcat taattaacgc catgctcaga agatcacgcc
    10381 cgggatcggc gtcaggctca tcgccgtttt caccatatag gtgcggctgc acattgctgg
    10441 gactaccagg aagataaatg ccatcgagtt tcggcaaaag ttgttcaagt aatgacggtt
    10501 ccgccagcgc atgtggtagc gcaattggca agccgcctgc gtggatgatg gcattcaggt
    10561 acttttcttg cagagtctgg gtcgcatgac ccttaagcct gttcctgcac attacgacac
    10621 cgataaccgg attgttcatt atattttcca tgctcaatct cacaaagtgg actaaattat
    10681 cgccattact gcggtttaaa tgacctttat gttcaatatt ttttcaatct agcagtggtt
    10741 tttcattttt gcaaaccaaa tttaacattt gacaaacatt tagtttgcat acagattcga
    10801 atggtggtca ttatatttta cgctttgata acgagcggaa aacaaaccaa aggcgaagaa
    10861 tcatggaaac caatatcgtt gaagtagaga actttgttca gcagtcagaa gagaggcggg
    10921 gtagcgcctt tacgcaggaa gtgaaacgct acctagagcg ctacccgaat acgcaatatg
    10981 ttgatgtttt acttactgat ttgaatggtt gcttccgtgg aaaacgtatc cctgtttcaa
    11041 gcctgaagaa gttggagaaa gggtgttatt tcccggcctc ggtgtttgca atggatattc
    11101 tgggcaacgt agtagaagaa gcgggtctgg gtcaggaaat gggcgagccg gatcgtactt
    11161 gtgttcctgt tctcggttcc ttaacccctt ctgccgccga tccagagttt atcggtcaga
    11221 tgctcctgac catggtcgat gaagatggcg ctccctttga cgctgagccg cggaacgttc
    11281 ttaaccgcct gtggcagcag ctacgccagc gcggattgtt cccggttgta gcggtagagc
    11341 tggagttcta tttactggat cgtcagcgcg acgctgaagg gtatctgcaa ccgccctgcg
    11401 cgcccggcac cgatgaccgc aatacacaaa gccaggttta ctccgttgat aacctcaacc
    11461 acttcgccga tgtgctcaat gatattgatg aactggcgca gttacagtta attcccgccg
    11521 acggtgcggt cgccgaagcc tcgccgggtc agtttgaaat caacctttac cataccgata
    11581 acgtgctgga agcctgcgat gatgcgctgg cgctaaaacg cctggtgcgt ctgatggcag
    11641 aaaagcataa gatgcacgcc acttttatgg cgaagccgta tgaagagcac gcgggcagcg
    11701 gaatgcatat ccatatcagt atgcaaaata accgtggcga gaacgtgctt tctgacgcgg
    11761 aaggcgaaga ttcgccgctg ctgaaaaaga tgctcgccgg gatgattgac ctgatgccgt
    11821 catcaatggc gttgctggca ccaaacgtga actcgtatcg ccgcttccag ccgggaatgt
    11881 atgtgccgac gcaggcgtcg tggggccata acaaccgcac cgtcgccctg cgtattccgt
    11941 gcggcgaccg tcataatcac cgcgtggaat atcgcgtggc gggtgccgat gccaacccat
    12001 atctggtgat ggcagcgatt tttgccggta ttttgcatgg ccttgataac gagctgccgt
    12061 tgcaggaaga agtcgaaggc aacgggctgg aacaggaagg cttacccttc ccgattcgcc
    12121 agagcgatgc cctgggtgag tttatcgaga atgatcacct gcgccgctat ttaggcgaac
    12181 ggttttgcca tgtgtatcac gcctgtaaaa atgatgagct gttgcagttt gagcgcctca
    12241 tcacagaaac agaaattgag tggatgttga aaaacgcgta attccccatg cggggttgtt
    12301 tcatcggcct gagcggagcc aggcctgaca tttgctggta atccagacat ttcccggatg
    12361 tcgcgttatg gagcgcaggc ggcaacgggc ttccgttaac gattaagact gtgacgagga
    12421 aatgagatga tgccgatgat taaatccccg catggcgagg gtggctgcgt atgcgcccct
    12481 cctgctactg actggactcc gccccctttg cttcccttgc taaacaggtt tgatttcagg
    12541 ttgacacgac cgcaaacctt attacgcagg ggaggcagca attatggcta ttaattcacc
    12601 actgaatatt gctgcgcaac ccggcaaaac ccgtctgcga aaatcactga aattgtggca
    12661 ggtggtaatg atgggtctgg cctatctcac gccgatgacc gtatttgata cttttggtat
    12721 tgtctccggc attagcgacg gtcacgttcc ggcgtcctat ttgctggcgc tggcgggcgt
    12781 gctgtttacc gctatcagct acggcaaact ggttcgccag tttccggagg ccggttcggc
    12841 ctatacctac gcgcaaaagt cgattaatcc gcacgtcgga tttatggtcg gctggtcatc
    12901 actgctggat tatctctttt tgccaatgat caacgttttg ttggcgaaaa tctatctctc
    12961 cgccctcttc ccggaagtgc cgccgtgggt gtgggtggta accttcgtcg ccattttaac
    13021 cgccgcgaat ctgaagagcg tcaacctggt cgctaacttc aataccctgt ttgtactggt
    13081 gcaaatctcc atcatggtgg tgtttatctt cctggtggtt cagggactgc ataaaggaga
    13141 aggcgttggc accgtctggt cacttcagcc gtttatcagc gagaacgcgc acctgatccc
    13201 gattattacc ggtgcgacga ttgtctgttt ctcgttcctc ggtttcgatg cggtgaccac
    13261 gctttcggaa gagacgccgg acgccgcacg cgtgatcccg aaagccatct tcctgacggc
    13321 ggtatacggc ggcgttatct ttatcgcggc gtcgttcttt atgcagctgt tctttcccga
    13381 tatcagccgc tttaaagacc cggacgccgc actgcctgaa attgcgctgt acgtcggcgg
    13441 taagctgttc cagtcgattt tcctttgcac cacgtttgtg aacacgttag cgtctggcct
    13501 ggcctcacat gccagcgtgt cacgtctgct gtatgtgatg gggcgcgaca atgtgtttcc
    13561 ggagcgcgtg tttggctatg tgcatccgaa atggcggact ccggcactga acgtcattat
    13621 ggtcgggatt gtcgcgatgt cggcgctgtt cttcgattta gttaccgcca cagcgttaat
    13681 taacttcggc gcgttggtag cgtttacctt cgttaacctg tcggtgttca accacttctg
    13741 gcggcgcaaa ggaatgaata aaagctggaa ggaacacttc cactatttat taatgccgct
    13801 ggttggcgcg ctgacggtgg gcgtgctgtg gattaacctc gaagccacct cgctgacgct
    13861 gggtctggta tgggcttcgt taggcggcgc atatttgtgg tatctgatcc gccgctatcg
    13921 caaagtgccg ctgtacgaag gtgacagaac gccagtgagc gaaacgtaat agtcggatgc
    13981 gcgccgcatt atcctgcctg agggtaatgc gccgcgccat taactataaa aagatgtatg
    14041 acccggatca cgagtgctat catccgccct tatgcttagc gcattaaccc caggggcaaa
    14101 taatgaacca cgatatcccg ctaaaatatt ttgatattgc cgatgagtac gcgaccgaat
    14161 gcgcagagcc tgtcgcagac gctgaacgca caccgctggc ccactacttc cagttactgc
    14221 tcacccgttt gatgaacaat gaagagatca gcgaagaagc ccaacatgaa atggctgctg
    14281 aagcggggat taatcccgta cgcattgatg agatcgcaga gtttttgaat caatggggta
    14341 atgagtaatt atttaattgc agacgatcgg tcagtatcgt tgtccggata aagcgttcac
    14401 gccgcatccg gctaacaatg acacgcactg cagcgtgctt taatgggttt ctcacgatcg
    14461 taccgctctg ctcctcgcta agttgttctg tgagttatag ttatagagaa atcacgggta
    14521 cgaatgttga acagatggcg acgccacctg ttcaacgacc aaccacacgg ccctcattac
    14581 gacccctgaa cacaaacggt aatggcaaat gtcattaaat attagtaaga tagcctcttt
    14641 acataaaatc ccttaatatt atgcgccagg tattatcgtc tcttttggtg attgctggac
    14701 ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc gcatgctgat atccgcgaca
    14761 gcggttttgt ctattgcgtc agcgggcaag tcaacacctt taacccatcc aaagcgagca
    14821 gtgggttaat tgtcgatacc cttgccgccc agttttatga tcgactgctg gatgtcgatc
    14881 cctataccta tcgcctgatg ccggaacttg ccgaaagctg ggaagtactc gacaacggcg
    14941 cgacctatcg cttccacctg cgtcgcgatg ttccgtttca aaaaaccgac tggtttactc
    15001 ccactcgtaa aatgaatgcc gacgatgtgg tgtttacctt ccagcgaatt tttgaccgca
    15061 acaacccgtg gcataacgtc aacggcagca acttccccta cttcgacagc ctgcaatttg
    15121 ccgataacgt gaaaagcgtc cgcaaactgg ataatcatac cgttgagttc cgactcgccc
    15181 agccggatgc ttcttttttg tggcacctcg caacccatta tgcttcggtc atgtcggcag
    15241 aatatgcccg gaagttagag aaagaagatc gccaggagca actcgaccgt caaccggtcg
    15301 gcaccggtcc gtatcagttg tcggaatacc gcgccgggca atttattcgc ctacaacgtc
    15361 atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt agtggtggat ttaggctccg
    15421 gcggcaccgg acgtctgtcg aaactcctga ccggggaatg cgacgttctg gcctggcctg
    15481 ctgccagcca gctatccatt ttgcgtgacg acccgcgctt gcgtttaacg ctgcgtcctg
    15541 ggatgaacgt cgcctatctg gcatttaaca ccgccaaacc gccgctaaat aatcccgctg
    15601 tccgccatgc actggcactg gcgattaata accagcgcct gatgcaatcc atctattatg
    15661 gtacggctga aacggcggcc tctattttac cgcgcgcctc gtgggcctat gacaacgagg
    15721 ctaaaattac tgaatacaat ccggcgaaat cgcgcgaaca gttgaaggcg ttggggctgg
    15781 aaaacttaac gctgaaactg tgggtgccca cacgttcgca ggcgtggaac cccagtccac
    15841 tgaaaactgc cgaactgatt caggcggata tggcgcaggt tggcgtaaaa gtggtgattg
    15901 tgccggtaga aggtcgcttt caggaggcgc ggttgatgga tatgagccat gatctgacgt
    15961 tatccggttg ggcgacggac agtaacgacc cggacagttt cttccgtccg ttactgagct
    16021 gcgcggcaat tcattcacag accaacctcg cccactggtg cgatccgaaa ttcgacagcg
    16081 tgttgcgtaa ggcgctctcc tcgcagcagc tggcagcgcg tattgaagcc tatgacgaag
    16141 cgcagagtat tctggcgcag gaattaccta ttctgccgct ggcgtcgtca ttgcgtttgc
    16201 aggcctatcg gtacgatatc aaaggtctgg tacttagccc gtttggtaac gcctcctttg
    16261 ctggggtgta tcgcgagaaa caggatgagg tgaaaaaacc atgattatct tcaccttacg
    16321 ccgcattttg ttattgattg tcaccttgtt cctgctgacc ttcgttggct tcagtttgag
    16381 ctatttcact ccacacgcgc cgttacaagg cgcgtcactg tggaatgcct gggtgttctg
    16441 gtttaacggc ctgatccact gggattttgg tgtttccagc attaacggtc aaccgattgc
    16501 cgaacagtta aaagaggtct tccctgcgac gatggaactg tgcatcctcg ccttcggttt
    16561 tgcgctgatt gttggcatcc ccgtggggat gattgccggg attacgcgcc ataagtggca
    16621 ggataacttg atcaacgcca tcgccttgct gggcttttca atcccggtgt tctggctggc
    16681 gcttctgttg acgctgtttt gttcactcac gctaggttgg ttgcccgttt cagggcgttt
    16741 cgatctgctg tacgaagtga aaccgattac cgggtttgcg ttgattgatg cctggctttc
    16801 ggactcacca tggcgggatg aaatgatcat gagcgcaatc cgccatatga tattgcccgt
    16861 gatcaccctg tcggttgcgc caacaactga agtgatccga ctgatgcgta tcagcactat
    16921 cgaagtgtat gaccagaact atgttaaagc ggcggctacc cgcggtttgt cgcgctttac
    16981 tattttgcgt cgccacgttc tgcataacgc gttaccgccg gttattcctc gcctgggctt
    17041 acagttttcc accatgctga cgctggcgat gatcaccgaa atggttttta gctggccggg
    17101 tttagggcgc tggttaatta acgccatccg ccagcaggat tacgcagcca tttccgccgg
    17161 agtgatggtg tgtggctcac tggtcattat tgttaacgtg atttctgata ttttgggtgc
    17221 catggctaac cctctgaaac ataaggaatg gtatgcctta cgatagcgta tacagcgaaa
    17281 agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt ttatagtgac gcctctgcga
    17341 tggtcggcct gtacggctgc gcggggctgg ctgtactgtg tatttttggc ggctggtttg
    17401 cgccttacgg catcgaccag caatttctcg gttaccaatt attgccgccg tcatggtcgc
    17461 gctatggcga agtttctttc ttcctgggga ctgacgacct cggacgcgat gttttaagcc
    17521 gtttactgag cggagctgcg ccaaccgtgg gtggcgcatt tgtggtgacg cttgccgcga
    17581 cgatctgtgg cctggtactg ggcaccttcg ccggggcgac gcacggccta cgctcagcgg
    17641 tgcttaacca tattctggat accttactgg cgatcccttc gctgctgctg gcaattatcg
    17701 ttgtagcgtt tgccggaccg agtttgtccc acgctatgtt tgctgtctgg ctggcattgc
    17761 tgccgcgtat ggtacgttcg atttacagca tggtgcatga cgaactggaa aaagagtacg
    17821 ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat tctctggttt gctgtgatgc
    17881 caaacatcac cgccgggctg gtcaccgaga tcacccgcgc actgtcgatg gcaattctcg
    17941 atatcgccgc gctgggcttt ctcgatctcg gcgcacaact cccctcgcct gaatggggag
    18001 cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc gtggactgtc atgctgccag
    18061 gtgcggcaat tatgattagc gtgttgttgg ttaacctcct tggcgatggc gttcgtcgtg
    18121 cgattattgc gggggtggaa taatgccatt actcgatatt cgtaacctga ccattgaatt
    18181 taaaaccggt gatgagtggg ttaaagccgt cgaccgggta agtatgacct taaccgaagg
    18241 tgaaatccgc ggtcttgttg gtgaatccgg ttccggcaag agtttgattg cgaaagcgat
    18301 ttgtggggtg aataaagata actggcgtgt tactgctgac cgtatgcgtt ttgatgatat
    18361 cgatttgctg cgtctctccg cacgcgaacg gcgcaaactg gttggccata acgtgtcgat
    18421 gattttccag gaaccgcagt cgtgtcttga cccttcagaa cgtgtgggtc gccagttgat
    18481 gcaaaacatc ccagcctgga cctataaagg ccgttggtgg cagcgttttg gctggcgcaa
    18541 acgccgtgcc attgaactgc tgcaccgcgt ggggattaaa gatcacaaag atgcgatgcg
    18601 cagttttccc tatgagttga ccgaaggtga atgtcagaaa gtgatgatag ccattgcact
    18661 ggcgaatcaa ccgcgtctgc tgattgctga cgaaccgacc aactcaatgg agccaacaac
    18721 ccaggcgcaa atctttcgcc tgctgacgcg tctcaaccaa aacagcaata ccactatttt
    18781 gcttatcagc catgacttac aaatgcttag ccaatgggcg gataaaatta acgtgcttta
    18841 ctgcggtcaa acagtggaaa ccgcgccgag taaggagttg gtgacgatgc cacatcatcc
    18901 ttatacccag gcgctgatcc gcgcgatacc agacttcggc agcgcgatgc cgcataaaag
    18961 tcgcctcaat acgctgcccg gcgctatccc actgctggaa cagttaccga ttggttgtcg
    19021 tctggggcca cgttgcccgt atgcacaacg agaatgcatt gtgacgccac gtttgacggg
    19081 ggcgaaaaat catctctatg cctgtcattt cccgctgaac atggagaaag agtgagatga
    19141 tcgaaacgct gcttgaagtg cgtaatttaa gtaaaacgtt ccgctaccgg accggctggt
    19201 ttcgtcgtca gaccgtagaa gcggtaaaac ccttgagctt tacgctacgt gaaggccaga
    19261 cactggcgat tattggcgag aatggttcgg gtaaatccac gctggcaaaa atgctggcgg
    19321 gaatgataga gcctaccagc ggcgagttat tgattgacga tcatccactg cattttggcg
    19381 attattcctt ccgcagtcag cgtattcgca tgatttttca ggatccttcg acctcgttga
    19441 atccccgtca acgtatctcg caaattctgg attttccact gcgcctgaac accgacctgg
    19501 aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg tatggttggg ctgctaccgg
    19561 atcacgtcag ttactatccg catatgttgg cacccggaca aaaacagcgt ctgggtctgg
    19621 cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga tgaagccctc gcttcactgg
    19681 atatgtcgat gcgttcgcag ttgattaatc tgatgctgga gttacaggaa aaacagggca
    19741 tttcgtatat ttatgttacc cagcatatcg gaatgatgaa gcacattagc gaccaggtgc
    19801 tggtgatgca tcagggcgag gttgtcgagc gaggcagcac cgcggatgtg ctggcatcac
    19861 cgctgcatga actcaccaaa cggctgattg ccgggcactt tggcgaagca ttgacagcgg
    19921 atgcgtggcg taaagatcgc taattttctg aatcctcctt ccagattatt cacctcattc
    19981 tgacaagaga ttaaatcttg tcagaatgag gtgatggata aaataaaatc aaatgcgcgt
    20041 gatttaagaa gcaatctcac tttgcaggaa cgaaagctct ggcgatatct tcgcagccga
    20101 cgttttggtg atttcaaatt tcgccgtcaa catccggtgg ggagctacat tctcgatttt
    20161 gcttgctgct cggcgcgcgt agtcgttgag ctggatggtg ggcagcatga tttggcagtt
    20221 gcctatgata ccaggcgcac tagctggctt gagtcgcagg gctggaccgt gctgcgtttc
    20281 tggcataacg agattgattg taatgaggag gcggtgctgg agattattct gcaggaactg
    20341 aaccgccgat caccctcatc ctgaaagggc gagggggccg accgagccga atagctgttg
    20401 tggtgaaaac atggagacag tgctggagaa tattcggcaa ggtctgaacc atcccagcca
    20461 tcgccataaa agggttaggg gctgtatgag cctgtttgtt gctggggtaa caatatttgc
    20521 acaatatggt cccctcgccc ctctggggag agggttaggg taaggggaaa ggcgcccacc
    20581 ctgccgcagc ctgctccggt cggacctggc aactatagct actcacagcc aggttgatta
    20641 taataaccgt ttatctgttc gtactgttta ctaaaacgac gaatcgcctg attttcaggc
    20701 acaacaagca tcaacaataa ggattaaagc tatgggtttt ctttccggta agcgcattct
    20761 ggtaaccggt gttgccagca aactatccat cgcctacggt atcgctcagg cgatgcaccg
    20821 cgaaggagct gaactggcat tcacctacca gaacgacaaa ctgaaaggcc gcgtagaaga
    20881 atttgccgct caattgggtt ctgacatcgt tctgcagtgc gatgttgcag aagatgccag
    20941 catcgacacc atgttcgctg aactggggaa agtttggccg aaatttgacg gtttcgtaca
    21001 ctctattggt tttgcacctg gcgatcagct ggatggtgac tatgttaacg ccgttacccg
    21061 tgaaggcttc aaaattgccc acgacatcag ctcctacagc ttcgttgcaa tggcaaaagc
    21121 ttgccgctcc atgctgaatc cgggttctgc cctgctgacc ctttcctacc ttggcgctga
    21181 gcgcgctatc ccgaactaca acgttatggg tctggcaaaa gcgtctctgg aagcgaacgt
    21241 gcgctatatg gcgaacgcga tgggtccgga aggtgtgcgt gttaacgcca tctctgctgg
    21301 tccgatccgt actctggcgg cctccggtat caaagacttc cgcaaaatgc tggctcattg
    21361 cgaagccgtt accccgattc gccgtaccgt tactattgaa gatgtgggta actccgcggc
    21421 attcctgtgc tccgatctct ctgccggtat ctccggtgaa gtggtccacg ttgacggcgg
    21481 tttcagcatc gctgcaatga acgaactcga attgaaataa tcattctgtt ggtaaatatt
    21541 gggcggcgtt ctgccgcccg ttatctctgt tatacctttc tgatatttgt tatcgccgat
    21601 ccgtctttct ccccttcccg ccttgcgtca ggataacgat ttcctttacg accaaggagc
    21661 gcccatggaa caacgccaca tcaccggcaa aagccactgg tatcatgaaa cgcaatccag
    21721 tactgcggag tatgacgttc tgcctctggt cccggaagcc gcaaaggtca gcgatccctt
    21781 tctgctcgac gtgatccttg atgaagaaac gctggctccc ttcctttcat ggctggtccc
    21841 tgcgcgcgtt cttgcagtgg aattgttccc tgaccagctt accgtaaccc gttcacagac
    21901 cttcaccgct tatgaacgct tgtctacggc cctgacggtt gctcaggttt gcggcgtcca
    21961 gcggttatgt aactactatt cggcgcgact tacgccgctc cccgggcctg attcctccag
    22021 ggaaagcaat cataggttgg cacaaatcac gcaatatgcc cgccaactgg ctagctcgcc
    22081 ttctattatc gacaaccgat cgcgccagca tctgaatgac gtcggtctta ctgccgggga
    22141 ctgtgtgatc attaaccaaa tcattggttt tattggcttt caggcgcgga ccattgcgac
    22201 atttcaggct tatctcgggc acccggtacg ctggttaccc ggtctggaaa tacaaaacta
    22261 cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg cgaagcagct atgaggtgga
    22321 aaaactacct gaagagtaca caaaaagttc aacagcagaa ctttgccaac tggccgaaac
    22381 actctctctc caccctattt cactttccct tctcgaaagg ttgttaaaca gtacacgggt
    22441 taatacacag ccggataata agcttgcggc gttgttatgc gcgcggataa atggcagtcc
    22501 tgcttgtttt gccgcctgta tggattcatc aaatgaatat aaaaaaatca gcccccttct
    22561 gcgcaagggc gaaaatgaaa ttaaccaatg ggctgaccgt cattctgttg agcacgctac
    22621 cgttcaggcg atacaatggc tgacccgagc acccgatcgc tttagcgccg cccagttcag
    22681 ccctttactc gaacacgaaa aatcatcaac gcagattatt aatctgctgg tatggagcgg
    22741 gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt gagacatatt aaccttgccg
    22801 cgtcagacag attcgcgtaa aactgtcagc cgctctaatg gccaccaaaa tagacaatta
    22861 tgtttcagga caacccgctg ctagcgcagc ttaaacagca actgcattcc cagacgccac
    22921 gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg cttcctggaa gtcgacgcgc
    22981 aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt catgcatggc gaccgaatta
    23041 tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga gccagaagaa ctggttgaac
    23101 cgttcctgac tcgtttcgtg ggtaaggttc agggcaaaaa tgaccgtctg gccatcgttc
    23161 ctgatcatcc actcttaaaa gacgccattc cttgccgcgc agcccgtggc ctgaaccacg
    23221 agtttaaaga aggcgactgg gcggttgccg aaatgcgccg tcatccgctg aaaggcgatc
    23281 gttctttcta tgcagaactg acacaataca tcacttttgg tgacgaccac tttgtaccgt
    23341 ggtgggttac ccttgcacgc cataatctgg aaaaagaagc accagacggc gtcgctaccg
    23401 aaatgctcga tgaaggtctg gttcgtgaag atctgaccgc gctggatttt gtcaccatcg
    23461 acagtgccag cacagaagat atggatgacg cccttttcgc taaggcgttg ccggatgaca
    23521 aacttcagct gattgtggcg attgccgatc caaccgcgtg gattgctgaa ggcagtaagc
    23581 tggacaaagc cgcgaaaatt cgcgcattca ccaactatct gcctggcttc aacatcccta
    23641 tgctgcctcg cgagctttct gacgatctct gctcactgcg cgccaatgaa gtccgcccgg
    23701 tactggcatg ccgcatgacg ctctccgctg atggcaccat tgaagataat atcgaattct
    23761 ttgccgccac catcgaatcc aaagcgaagc tggtgtatga ccaggtttct gactggctgg
    23821 aaaataccgg tgactggaag cctgaaagtg aagcaattgc cgaacaagtc cgtttgctag
    23881 cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca cgcactggtg tttaaagatc
    23941 gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt gctggatatc gtcgccgagc
    24001 ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat tgccgctaac atttgtgcgg
    24061 cccgcgtact gcgcgataag ctcggttttg gcatctataa cgtgcatatg ggctttgatc
    24121 cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca cggtctgcat gtcgatgccg
    24181 aagaagtgct cacgctggac ggtttctgca aactgcgtcg tgaactggat gcgcaaccaa
    24241 ctggtttcct cgacagccgc attcgtcgct tccagtcatt tgctgaaatt agcactgaac
    24301 ccggtcctca ctttggcctc ggtctggaag catacgccac ctggacttcg ccgatccgta
    24361 aatatggcga catgatcaac caccgtctgc tgaaagcggt tatcaaaggc gaaactgcga
    24421 cgcgcccaca ggatgaaatc actgtccaaa tggccgagcg tcgccgtctc aaccggatgg
    24481 cagaacgtga tgttggcgac tggttatacg cacgcttcct gaaagacaaa gccgggaccg
    24541 acacccgttt cgcggcggaa attgtcgata tcagccgtgg cggcatgcgt gtgcgtttgg
    24601 ttgataacgg cgctatcgcc tttattccgg cacctttctt acacgctatg cgcgatgaac
    24661 tggtttgcag ccaggaaaac ggcactgtac aaattaaagg tgaaacggtt tacaaagtaa
    24721 ctgacgttat tgacgtcacc attgccgaag tccgcatgga aacccgcagc attattgcgc
    24781 gcccggtcgc gtaatctcct ttcacggccc
//
LOCUS       DAZGVQ010000070        24530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000070 DAZGVQ010000000
VERSION     DAZGVQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.070"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            135..1457
                     /gene="yhjE"
                     /locus_tag="ACHLAG_002686"
     CDS             135..1457
                     /gene="yhjE"
                     /locus_tag="ACHLAG_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0655888.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(1691..3751)
                     /gene="yhjG"
                     /locus_tag="ACHLAG_002687"
     CDS             complement(1691..3751)
                     /gene="yhjG"
                     /locus_tag="ACHLAG_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP0655889.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLIYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(3821..4588)
                     /gene="pdeH"
                     /locus_tag="ACHLAG_002688"
     CDS             complement(3821..4588)
                     /gene="pdeH"
                     /locus_tag="ACHLAG_002688"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFP0655890.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            4820..5749
                     /gene="kdgK"
                     /locus_tag="ACHLAG_002689"
     CDS             4820..5749
                     /gene="kdgK"
                     /locus_tag="ACHLAG_002689"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFP0655891.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(5845..7341)
                     /gene="yhjJ"
                     /locus_tag="ACHLAG_002690"
     CDS             complement(5845..7341)
                     /gene="yhjJ"
                     /locus_tag="ACHLAG_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP0655892.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLADSTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(7562..8848)
                     /gene="dctA"
                     /locus_tag="ACHLAG_002691"
     CDS             complement(7562..8848)
                     /gene="dctA"
                     /locus_tag="ACHLAG_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFP0655893.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(9031..11019)
                     /gene="hmsP"
                     /locus_tag="ACHLAG_002692"
     CDS             complement(9031..11019)
                     /gene="hmsP"
                     /locus_tag="ACHLAG_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFP0655894.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(11101..14574)
                     /gene="bcsC"
                     /locus_tag="ACHLAG_002693"
     CDS             complement(11101..14574)
                     /gene="bcsC"
                     /locus_tag="ACHLAG_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFP0655895.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQHKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPANADAILGLTEVDIAAGDKAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQRTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(14556..15662)
                     /gene="bcsZ"
                     /locus_tag="ACHLAG_002694"
     CDS             complement(14556..15662)
                     /gene="bcsZ"
                     /locus_tag="ACHLAG_002694"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFP0655896.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPSSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(15669..18008)
                     /gene="bcsB"
                     /locus_tag="ACHLAG_002695"
     CDS             complement(15669..18008)
                     /gene="bcsB"
                     /locus_tag="ACHLAG_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFP0655897.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(18019..19119)
                     /locus_tag="ACHLAG_002696"
                     /pseudo
     CDS             complement(18019..19119)
                     /locus_tag="ACHLAG_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312440.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
     gene            complement(19391..19975)
                     /locus_tag="ACHLAG_002697"
     CDS             complement(19391..19975)
                     /locus_tag="ACHLAG_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001056416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655898.1"
                     /translation="MNMKKSSFLDEGKEPVIERIFRLAERYKSRSEAARAWGINIQTL
                     QNYYKRRNNQPPPQPRKHHLQNIAEKEHVSMEWLIYGYNNESITKKELLEQIERTKKQ
                     DVNDGDSIIMRAWDSLTSSEQEILSNLLIRKGAELLTILLDTDIQKLHSLSGVKRALA
                     LSLQELPDDTVREIYEECEAKVNHLNVTQKKAGA"
     gene            20142..20390
                     /locus_tag="ACHLAG_002698"
     CDS             20142..20390
                     /locus_tag="ACHLAG_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0655899.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            20392..22481
                     /locus_tag="ACHLAG_002699"
                     /pseudo
     CDS             20392..22481
                     /locus_tag="ACHLAG_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
     gene            22565..23497
                     /locus_tag="ACHLAG_002700"
     CDS             22565..23497
                     /locus_tag="ACHLAG_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0655900.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELAEELGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAVDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWITASGEGAVLTEKHINAAFKEVYTNPELLSQV"
     gene            23500..23721
                     /locus_tag="ACHLAG_002701"
     CDS             23500..23721
                     /locus_tag="ACHLAG_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655901.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATEVYRGICRSVGAKQVR"
     gene            23734..23988
                     /locus_tag="ACHLAG_002702"
     CDS             23734..23988
                     /locus_tag="ACHLAG_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655902.1"
                     /translation="MNMQSCGKKMNLFDSLNSARRLTELAGAVLERSKRYPQRYALKT
                     TPPVSEVKGTGEIEITIQTNGLRRRVKATRISGCTVYWEE"
     gene            23990..24271
                     /locus_tag="ACHLAG_002703"
     CDS             23990..24271
                     /locus_tag="ACHLAG_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFP0655903.1"
                     /translation="MKRNLIAWAWSSGLIELGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            24268..>24530
                     /locus_tag="ACHLAG_002704"
     CDS             24268..>24530
                     /locus_tag="ACHLAG_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655904.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGISLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRDFAELVAMKASALMKDMSMRSGVIKGNEFN"
BASE COUNT         5757 a         6660 c         6387 g         5726 t
ORIGIN      
        1 agaaaaatca ctggcctaaa cattatcccc tttttgcctg gtttttgacc atttccgcga
       61 tttgttacac attgaaatat cacttttgct gtgcgtaata tggctattcg ttagccaaaa
      121 aataagaaaa gattatgcaa gcaacagcca caacactcga ccacgagcaa gaatacacgc
      181 cgatcaactc gcgtaataaa gtccttgtcg cctctctcat tggcacggcc attgagttct
      241 tcgactttta catttacgcc actgcggccg ttattgtgtt tccgcatatc ttcttcccgc
      301 agggcgatcc aacggcagca acgctacagt cgctcgccac cttcgccatc gccttcgtcg
      361 cgcgccccat tggctctgcc gtttttggtc attttggcga tcgcgttggg cgtaaagcga
      421 cgctggtcgc ctcgttgcta acgatgggga tttcgaccgt ggtgattggt ctgctgccgg
      481 gctatgccac gattggtatt ttcgccccgc tgctgctggc gctggctcga tttggtcagg
      541 gtctgggctt aggcggtgaa tggggcggcg cggcgctgct ggcgactgaa aacgccccac
      601 cgcgcaaacg tgcactgtat ggctccttcc cacagctggg cgcaccgatt ggcttcttct
      661 ttgccaacgg cactttcttg ctgctttcct ggctactgac cgacgagcag tttatgagct
      721 ggggctggcg tgtgccattt atcttctcgg cggtgctggt cattatcggc ctgtatgttc
      781 gcgtgtcgct gcatgagtcg ccggtgtttg agaaagtcgc taaagcgaaa aaacaggtga
      841 agatcccgct gggtacgctg ctgaccaaac atgttcgcgt aaccgtactg ggtacgttca
      901 ttatgctggc aacctatacg ctgttttaca tcatgacggt ctactctatg accttcagta
      961 ccgccgccgc gccagttggg cttggcctgc cgcgtaacga agtgttgtgg atgctgatga
     1021 tggcagttat tggttttggc gtgatggtgc cagtcgccgg attactggct gatgcctttg
     1081 gtcgccgtaa aagcatggta atcatcacca cgctgatcat cctgttcgcg ctgttcgcct
     1141 ttaacccgct gctcggttct ggcaacccga ttctggtttt tgccttcctg ctgctggggt
     1201 taagtctgat gggactgacc ttcgggccga tgggcgcgct gttaccagag ctgttcccga
     1261 cagaagtgcg ttacaccgga gcatcgttct cttacaacgt agcgtcgatt ctcggggctt
     1321 ccgttgcacc atatatcgca gcctggttgc aggctaacta cgggttaggt gcggtggggt
     1381 tatatctggc ggcgatggct ggcttgacgt taatcgccct gctgctgacc catgagacgc
     1441 gacatcagtc gttgtaagta tcgtgtgggc agccaccctc accccagccc tctccctgaa
     1501 agggagaggg agaaaagcgg agcgagatgt tatatcgcga acataacagc cctcccccag
     1561 aaagtgaacg gaaaagcggt actcgttgtt tcaacacgca caaaacagtc ccctcgcccc
     1621 tttggggaga gggttagggt gaggggaaca cgctgctccc gaactcacca gcaaacacaa
     1681 tccaacctca tcacttcttc atctgcgaca atatcgtccg acactgatta gcctcccctt
     1741 cggaaggtga gatcagtgcc agtaacgccg ccgccggtgt aaccagcgtt gccagtgccg
     1801 cagcaacagc accacgggca atcaacggcc cggctttcac cccagcctgc ggatttttaa
     1861 acgtcccacg cacatacagc ggcgaacgca gtgtgataat ccgaattcct ttactctccg
     1921 gatcaatagt caaatccagc tgttcggaag caaaacttgc cgtgccggta acgttaatca
     1981 acgcgttctc agtatcgaaa gcaaagatct gcgggcgcgc cacgccgttg gcaatatcca
     2041 gattcgccgc cgcgcagttc acccgcacct catcatcacc aaatatcgca ccgacaatgt
     2101 agttgccgac attcagccca acaatctcca tcaggttgcg gctcaccagc ccgtcattca
     2161 tcaacagttt caggttgccg ttactgttgc ctaaaagtgc cgccaccgag ttaccgctac
     2221 cgcgcagttc cgcgtcaccg ttcatttccc ccagcgtctt ctgcatcagt tccacatcgg
     2281 gcatcagttc tttcagtttc agtcgacgag cctgaatatc tgcccgcccc tgcatcggct
     2341 ttttatcgcc ttccagatga atattcgccg caatgctgcc acccgccatg ccaaatttca
     2401 gcggttgcag gcgcaggtca gcatttttga ggatgatatg agtagaaaga tcgctaatcg
     2461 gcaggctact gccatgctca atgcgccgcc ctttgaagcg aacatcggca tccataacgt
     2521 cccatttatc ggtttcgaag cggtcataag gcagcacttt gcccgcaggc tgaacgcttt
     2581 tttcgccctt cttctgttca gaccgtttcg acttttcagc ccctttcccg gaatcaacgc
     2641 caatcaacgg tcccaggtcc gccagccgca attgccgcga ctcgacatca ccttccagtt
     2701 ttggtcgtgg cttgccggtg gtgtagatca gagaaccgtg gatatcgcta tcgccaatgc
     2761 gcccattaaa accgcgataa tcaaagaccg acgatttttc agtgtcgatt ttcgctacca
     2821 gccgaccatc tgtttcaaac ggcggggtat cgggcagcag aacgcccgtc agttcataga
     2881 gatcacccag tgaatcgcca gaaaatttaa gccgtaaatc gacaccgccc atcttcattg
     2941 ggtcattcac gacgccatca aaagcaacac gggtattacc tgaacggaaa tcagcctgta
     3001 ccggaaacgg cgtcccttcg ccacgcagcg ccagcatacc gcctattttt cccgtaccag
     3061 tgagcggttc accgttataa cgtccctgcg ccttcaggcc aaaaacgtaa tcgcccacct
     3121 tttctttatc cgctttacct ttcgatccag taacttcgct gaacggcagc ggcttaccta
     3181 acggatcgac aaaaatttcc agatccgctt tgctcacttt gtcatcaatg gcgatccgcc
     3241 cttgatcgaa aagaatatta tccagccgaa acgaccatgc cgacggcttt gcattcgcgt
     3301 ctttgttatc atcgttggcg agattaaacg tccagttatt gttcttttca gagaggcgaa
     3361 tcaggcgcgc gtcgggcttt tcgagcttga tccacggcag ccagaccgtt ttggtcagca
     3421 gcgccagcgg ggccagcgtt gcctctacgc gtggcaaatg caccatcgtg acttcgggaa
     3481 tatccggtgg attgccaaga atgatgtctt ccgcgtgtac atggggccat ggcacccagc
     3541 tgcgccagcc agtttcttgt ttttgccgct cccacaccac gcccagatcg ccacggatag
     3601 cgaacggacg attcaactcc gcagagactt tctggttgat ggtcggtttg agtcgattcc
     3661 agtcaaaagt tgcaatcaaa atgatcgcca cgacaatcaa caacaagaaa gcccctgaaa
     3721 tcgcagcggt tattttgcct gccttgctca tcgttcgctt cctcatattc ttcctgtgcc
     3781 agtcctaaag atagtccagc caggcggaaa atgaggcagc ttatagcgcc agaaccgccg
     3841 tattcagcgt ttctatcggt gccgggcgtg aaagaaacca gccttgtgcg gcgaatgcgg
     3901 gcgagttctg aacatcacgc cactcttccg gtgtttctac gccctcgaca atcaccccgc
     3961 gacaatagcg attcattaga tgtaaaagct gagaaaagag tgtgcggcct tccggcgact
     4021 gacgcagcat cacaaacagt tctcgcgcga ttttgatgta gtcataacgc acttcactta
     4081 gtgcagagaa atttgccatc ccggtaccaa aatcatccag ccacagcggg ccaaattcac
     4141 acatcgaggc aaaggttgaa tctttcggca gacggatatg ctccaccagt tcgaaacgca
     4201 gccagggaag acgctcaatc tggcgcagga tttttggttg ctgacgcagg gcgatgagcg
     4261 tagggccatc aatattgacc gatgccagca ggccgtgctc tataaagaag tcggcttttt
     4321 gcgccagcaa atcaatctgc tctttcacaa cctccatccg atggctgacg gtgatttcag
     4381 taaaatagcg atccggcggc aggcgttgcg aagggttcaa gggatgcgtg accaccgtta
     4441 atagctccac ggccattaac cgcccgcatg tttgatagat cggctgccag gtgtaagcac
     4501 gctcacactg caaccaaaaa cgccgttcct gcaagctctc gatgcttgct tcagggttgc
     4561 ttattcgctg gataacctgc cttatcatca aagatgtcct gtgtttaaaa gtgatgctgc
     4621 ccggactcga caaagattat cggcgcgcaa gggcagaact ttaccgcctg gccggggtaa
     4681 atttcccctc tccacaacgg aatctttcag gaatacgttc tggctcaaaa aaataatgga
     4741 acactgtttt aatatggttg accagcaaac caccacagcg caaactaacg ctaatttttt
     4801 acagatcagg ttcacgacta tgtccaaaaa gattgccgtg attggcgaat gcatgattga
     4861 gctttccgag aaaggcgcgg acgttaagcg cggtttcggc ggcgatacac tgaatacttc
     4921 cgtctatatc gcccgtcagg tcgatcctgc ggcattaacc gttcattacg taacggcgct
     4981 gggaacggac agttttagcc agcagatgct ggacgcctgg cacggcgaga acgttgatac
     5041 ttccctgacc caacggatgg aaaaccgtct tccgggcctt tactacattg aaaccgacag
     5101 caccggcgaa cgtacgttct actactggcg taacgaagcc gccgccaaat tctggctgga
     5161 gagcgagcag tctgcggcga tttgcgaaga gctggcgaat ttcgattatc tctacctgag
     5221 cgggattagc ctggcgatct taagcccgac cagccgcgaa aagctgcttt ccctgctgcg
     5281 cgaatgccgc gccaacggcg gaaaagtgat tttcgacaat aactatcgtc cgcgcctgtg
     5341 ggccagcaaa gaagagacac agcaggtgta ccaacaaatg ctggaatgca cggatatcgc
     5401 cttcctgacg ctggacgatg aagacgcgct gtggggtcaa cagccggtgg aagacgtcat
     5461 tgcgcgcacc cataacgcgg gcgtgaaaga agtggtggtg aaacgcgggg cggattcttg
     5521 cctggtgtcc attgctggcg aagggttagt ggatgttccg gcggtgaaac tgccgaaaga
     5581 aaaagtgatc gataccaccg cagctggcga ctctttcagt gccggttatc tggcggtacg
     5641 tttgacgggc ggcagtgcgg aaaacgcagc gaaacgcggg cacctgaccg caagtaccgt
     5701 tattcagtat cgcggcgcga ttatcccgcg tgaggcgatg ccagcgtaaa cgcagcatat
     5761 gcaaaaggct ggataaagtg ttacccctct atccagctca tccataaata cctgatggtt
     5821 gtgctaactc atcaccatca ggcattactg tgcaggtgga atatccgtca cttcaggatg
     5881 tacgtcatcc gtggcgacag aggtggcggc agcagcagtc gatggggcca tgatttgatc
     5941 ccaggccgcc tgcaacgctt tcatgttaaa ttccggctcg cctttcggct gcagcagtat
     6001 taacgccata tcattcgaca actgctgacg cagatcctga tttaacatct ccaccgtcag
     6061 gctgttaagg aaatcctgcc gcagtttctg atactgctcc ggcgcgatat cgacgacctg
     6121 attttgcaac gaacgcatcc gctgacccat cagaatatcg gtatcagccc gcgcataggc
     6181 ggcaaacagt ttctgcagct ccagtttctt ttgcgccact aacgcattga actcttcttc
     6241 cggcagacct ttatcgcgaa ccttcgccag ttcacgcgcc accagattaa ggttgctgtt
     6301 cagcttgtcg tttggtgatt cgatgttgat ggcacactgc gcacgcagat acagcacacg
     6361 gcagtcaaat ccaagaccga tgtctttgct gttactggcg cttaacgctt gctgaacatg
     6421 ccagaacagc gcctcgcggg ccaggtccgc acgccagtag cgcagcagtg cggctgattc
     6481 acgaatcggc tgccacggcg tatcccacat gatagataac cggtcctgac gcaccgcgtc
     6541 agtcataata ctcaccgctt ccgcacgcag cggagaaagc gtcggcaccg gagctggcgt
     6601 ttcacgtttg cctttcagtt cgccaaacgt tttgtttatt tggtcgacaa ccgagcgcgc
     6661 atccacgttt cccaccacca gtagcgtcat tgcatccggg gtgtaccatt tctgatagaa
     6721 atctttaatt ttttccgctt caacgggttg tttcagcgga tcggcaggat cgtgacctaa
     6781 caaggttgac cctttcagac gatagcgcca ccagccctct ttagtatcgg caggccaggt
     6841 tgccaccatg tcctgacttt gcagcgcgtg gttgatggtt tctggtgtga tggtcaattt
     6901 gccagtggca tttgccagat aagagagcgc ttctttcagc aaatcgttac ggttattggg
     6961 caaactcaga ttaaacagcg tggtgtcata agagacaatt accggcggca tcgggcgttt
     7021 agggtcgatc ccctgctgcc acaatgaacg cgcctgtgct gcgtcaaggc caccgctttg
     7081 cgttagcgca atacgaggga tggcgtgact gtaaccgctc tgttgtgtac tatcggcgag
     7141 cgaaccggta ttaaccagca ggcgaatttc aacacgatcg ctgggacgct ggggggtggt
     7201 cagcacttgc cactgtaaac cgttggaaag cgtcccctgt tgccatgctg gatcaggctg
     7261 gagcgcatct gcctgcacat agccagcagt ggccatcatc agcaaaccgc ccgctaaaag
     7321 tcgaattttt gtgccctgca tgtgaacccc tgatcaacta tcctggtaat aaaaaaactg
     7381 cccgttgtcg gtcagtctaa atgacgttaa aaacacttcg tgttagaccg caagaacatg
     7441 aaaatgtcac ggaagaagtg aaataaacct gaactcaccc aggtggtaag ttcaggcaca
     7501 ggggtcaatt atgcgcaaac acccgcactc ggggaaggga gtgcgggcat aagtgatgag
     7561 attaagagga taattcgtgc gttttgccat ccggcgcacg attattcagc acatcgtcca
     7621 gttttttgtg gtccagttct ttcacccact tagcaacgac aatggtcgct acgccgttac
     7681 cgaccaggtt agtcagcgca cgagcttctg acataaagcg gtcgataccg aggatcagcg
     7741 ccagacccgc taccggcaaa tggcccaccg cagagagcgt cgccgccagc acgataaagc
     7801 cactacccgt tacccctgcc gcccctttag aagaaagcag caacacgatt aacagcgtga
     7861 tttggtggac gatatccatc tgactgttag tggcctgggc gataaacacc gccgccattg
     7921 tcaggtatat cgatgtgcca tcaaggttaa acgagtagcc tgtcgggatg accagcccca
     7981 ccaccgattt gcggcagccg agtttctcca tcttgtcgag catacgcggc agcgccgact
     8041 cggaagatga agtccccagt acaatcagca gttcttcacg gatgtagcgg ataaatttga
     8101 agatactgaa accagtcgct ttagcgattg aacccaatac cagcaccaca aacaggatac
     8161 aggtaatgta gaaacagata atcagctgcc ccagttgcac cagtgtgccg acgccgtatt
     8221 taccgatggt aaacgccatt gccccgaacg caccaatagg cgccagacgc atgatcatat
     8281 tgatgatgcc gaagatgacc tgcgagaaac tttcgatgac gttaaaaatc agttggcctt
     8341 tgctgcccag acggtggagc gcaaaaccaa acagtacggc aaacagcagc acctgcagaa
     8401 tgttaccgct ggcaaatgcg ccaatgacgc tcgccgggat gacatccata atgaaggcga
     8461 caatgccctg gtctttcgcc tgatcggcgt aaaccgctac cgctttcgca tcaagcgttg
     8521 ccggatcgac gttcattccg gcaccaggct gcacgacgtt aacgatgata agaccaataa
     8581 tcagcgcgat ggtactgaca atttcaaagt aaagcagtgc gactgcgccg gtacgaccga
     8641 ccgccttcat gctttccatg cccgcaatgc ccgttacgac ggtacaaaag atgacaggag
     8701 cgatgatcat cttaatgagc ttaacgaagc cgtcgccaag cggtttcatt tgctcgccta
     8761 tttcaggata gaaatggcca aggagaatac caatggctat cgctgtcagg acctgaaagt
     8821 aaaggctttt aaacagagag gttttcatag ggtgtccttt agtaaaacca caggtcttgt
     8881 aaggttatgg ggtacctgcg gccttaaaat aacacccaga caacatcaca gaaatgtacc
     8941 tggatcataa ttgaaacaaa aaggttaaaa agtttgagct ggctcgcaca aaccagcact
     9001 ttttaaagtt ttgtaatcag tttggggtag ctacttttct tccaggtaac tctcttcgaa
     9061 gatttcaata gggagtgggc gagcaaaaag gaagccctgg gcaataccaa cgcccgcttt
     9121 tgccagccag tcgcgttgtg cttcagtctc cacgccttcg gcaatcattt gtaagttcag
     9181 gctctgcgcc agcatgataa ttgcagcaat catgctgcta tcttccggca agccttcaac
     9241 aaacattttg tcgattttca gtacgtcgat tggcaacgat ttcatatgct gcagctgacg
     9301 cagccctgcg tagcccatgc cgaaatcatc cagcgccacc cgaactccgg cattgcgcag
     9361 cggacggagg attgccaccg cagcatgagg gtcgtcaata cgtcggcttt ctgtcacttc
     9421 cagaatcagt gttcccggct gaatgcgata gcgggttaac agttccagca tatccgccac
     9481 catatttggg tgcatcagtt gcagcgcaga gaggtttacc gacaagggca gcataatgcc
     9541 gcgctcttgc caggctgcaa gcaatcgaca ggactcttcc agcacccagt gaccgacggt
     9601 aaccatcagc ccacagcact caatgcgatc gattaagcca tccggcaggt cccaactgcc
     9661 atccggttgc tggatacgca gtaacacttc cgcactgacc agttttccgc tggtcatctc
     9721 gacctgtggc tgtaaccaaa tagcaaactg atgattttcc agtgcattaa ggatatcgct
     9781 ctcttccgtc aaccgcttct gggcggcttc catctgctgc ggatcaaaga actgaatctg
     9841 attcttgcct ttatggcgag cggtaaatgc cgcagaaata gcgcgactgt aaagctgttc
     9901 ggcggtgaga tcgccgtaga acatcgccac gccaatgcta cagtgcggac ggagttgaat
     9961 acgttcaatc ggcaggcgct cgctcatgat agtgagcact tgctgaccta aggtgattgc
    10021 gtgccacggt tcctgtacac cgttggcaat gacagcaaag tcataaccgc taatctgcgc
    10081 gaggatcata cgtggcgaca gtaccgattt gagtttttcc accagcgtca gcagcagaat
    10141 ttctcgttgc gcctctttca gcacgcccgc agtatcacgc agggtttcac aggtgatgat
    10201 catcagcgcg gtggtttgtt tacgcgcgac aacctgctcc agcatctcca tcagcaaggc
    10261 tttgttcggc aaatccgaca ccgggaagcg catcgcattt tcgttctgtt cttcataatg
    10321 gcgctgcagc aattgctggt tgaggttgta actgcgcacc aacataccga tttcatcgtc
    10381 ctgatgcaga cgcggtaatg ccagttggtg accaacaagc tcctgggctg ggatggcgtt
    10441 aagttcgcga gcaatattgc gtaacggatg caaaatcagg cggttaatgc accagctgat
    10501 ggcgacggtc aggataagcg acaaaagtaa gtaaatggtc actaacgttg agagggtgct
    10561 catcacgaac ttatacatac ggaaggaatc cgcctgtagt accagatagg caattggctg
    10621 cgggtttgcc ggacgttcga gcgagtaaac gcccagcgag atttgaaccg gtagctcaaa
    10681 caggcgagta accattaccg gcaccgggcg ctctggaata aaacttttac gcagcgcctg
    10741 gaactggtta ggcagcacta catcggcacg gctgaccacg cctgccggtt taatgctggc
    10801 aagaatagct tccgcttcgg gaatatcgcc tttcaaaata gctgaagata agggttcgcg
    10861 gacagagcga gcaatgcttt ccagttgcgt agccgtgtta tagcgattct gttggaccag
    10921 atggaacagc aaaacggtgc aaaaaataaa aacgaacacc aggacaacgg ctgccaccat
    10981 tgccatctgc ttgattgtta acgagcgact tacgcgcaaa ttgtctcccc ccggaaatcc
    11041 cagattgccg cccgagtata cccgatcgca gcggcattaa gagaggcgct atctgaaaac
    11101 ttaccagtcg gcgtaaggta tcagcggctg cggcggtaaa tccatgtcac cctgccatcc
    11161 ggcggcggaa taacgtacgt agagcaggaa atggctgggt gcgtaatctt tcgcctgctg
    11221 gatatcaatt gccgtgccaa caaaccagtt ggaagtaaca cgtcgttcaa gtaatgcccg
    11281 cgccgtgtag ccgaagccct gactgctgcc gccatcgttg gattgccgcg cagcttcttc
    11341 ctgccagtcg gtcgggatca gattcatcag cggataacgc ggcatggttt tggtgcgtga
    11401 atgcgaccac gagccagacg ctcccagctc ccacgaccag ttttccgtgc gctcccgcca
    11461 catcaccggt atggcaaacg acaggtattc ctgcggactg tagtaaccgc cctgaccgag
    11521 tgagtagcca ctcagatctt tgtcgtaatg ccagatcatg ttgttcaggc cgattgtgac
    11581 gcggcgattg ttctggttaa tgaccttata gtaatagccc gtcatccagc gcacgcgcca
    11641 gttatcttcg acatttttac cggttaactg gtcgccacta agcgatgccc agacgccgtt
    11701 tgcttcacct ttatcgtagc tcagacttag ccccacaccg tcggcacgta cgccacccca
    11761 ttttttcccg gtattgctcg gggagtcttt ttgcccacca aaggccagca aagaactgga
    11821 gatgggccga cggtgggcgt taacggtgta acccagcggc ccgatatcat cgctgtaact
    11881 gatgccgccg accacatcca ccacgttgaa gcccatcggc gtggtaccga tatcccagct
    11941 ccagacgtca tttcgccagc cgaccgccac gctggcaccg gaatccgact ggctgcggtt
    12001 gccgctacag tcctgtaatg tacaggtgcc ccagttgtca tcccatttgc catcggcatt
    12061 agtggagaaa ctgccgacgt tcatattgac gaaatcactg cgaaagaaca tccgcccgtc
    12121 agaatacggc gcatccacct gcaacatggt agtgtgcgct ttcagatcgg agtaaccacc
    12181 ggtgccgctc gaaccccagt aatcgtgctc aagggtgacg ttaagatcct gctggcgata
    12241 gaggtccgcc gcatcgctgc gcacgccgcg tttcagccag tcatctttct cgtcgttacg
    12301 ggtcagtcgg gtgaatgtgt cgttatcctg cggacgcgtc gtagtcacac cggatgcgac
    12361 catggcgtct ttgtaggttt ccagcgcctg ctttggatcg cccgcctgcg cttcaaactt
    12421 cgcaccgtcg cgcagcacca tcgcgctttc catcgacggt ggctgagatt ttgcctgcgg
    12481 gatcaactta ttaaacgtcc gctgcgctgc tgcggtatcg ccaagctgcg cctgtgccag
    12541 cgccacgcgc cgctgtgtgt tcagcgaggc gttatcggta gcgggcagtt tcgccagctg
    12601 gctacgtgcc gccgctttgt caccggcagc aatatccact tccgtcagac caagaatggc
    12661 gtcggcgtta gctggctccc gcgtcaggac attctgatat gcagcacggg cggcggtgta
    12721 atcacgtcgt tgttgcgccc agtcagccag cgtgaggtca atacgcgtgg aaggtggttg
    12781 ctgacgcagc atcgcttccg cttcagcttc tttgccgctt tctcgcaggc gattagcggt
    12841 ttccagcacc tgatcgcttt gcagtcgatt aaccagctcc tgaatattgc tgttccactg
    12901 cgcgcgcggc aggctgttga tatgcgccag cgccgctctg tcctggtcat gaccagagag
    12961 gtacagcccg taagcgtaaa cctgctccgg gtcgttcggc ttatgctgcg ccagattgcg
    13021 cattaacgta tcggcctggc tgcgttgtcc ggcctgccag agatcctgcg aaagtcggta
    13081 agtaatccat acgctgccgg ggtccagcgc cagtcgttgc cgctgaagtg ctgccgcctg
    13141 cgcccatttg ccctggtttt ccagtgcctc tgcctgctgt gccagacggt cgttttgcag
    13201 gctgcgttcg atatcatcaa tgctacgccg ctgactggca gagagcgagg cgataaacgc
    13261 ttcagctttt tctggcgatt gctggcggta aatatttgcc agcccgcgca cggcgttagt
    13321 gttgccgctg tccatacgca aggtctgctg ataataacgt tcggcggcgg gataatcttt
    13381 tcgcgccatc gccacatcgc ccagccccag cactgcataa ctgtcggtgt tatcgacatt
    13441 acgcgcctgc tggaacaggc gttctgcccg gtcaggatta ttggctttca gcgcagcatc
    13501 gccctgctgg atcgccagcc agtagcggtt tactttcagc agactgttcc atttgtcgtt
    13561 gttgctgctg tgcgggtcca gtgcgagggc tttttccaga ttcgccactg cattggcgcg
    13621 atcgcctttc tgagaatacg cctggcccag cgcccccaga gcttcactgt ctttcgggtt
    13681 cgcccgcacc gcctgttgta gttcgggaat ggctttaccc gccataccag agtccaccgc
    13741 cgctaaacct tgcgcacgag cgcggaaagc aggatcggcc agctgttttt gctgttctgc
    13801 cagttgcgat tgcgcagccg ccacgctatc gccatcacta aagatcgaga gatatttttt
    13861 cagcgccgac acactggcat cactgacggg catgtcttta atctgcccgt accagatttt
    13921 agaggcccct tcgcgcccgg cgttcgattt tgccatctgt tccaggacgg caaaaccttc
    13981 gtcacggcga tcgctactaa acagcaatag cgccagattg ttttgcaggc ccgtattgcc
    14041 cggtgcatcc gcattgatgc gttttaactg attaatcgct tcgccacggc gagccggaat
    14101 tttcgccacc gtactccagt actcgacggc aatgtcacct tccggcggcg caccgttgaa
    14161 cagtttgttg taactcgcca cagcttcttc tgcatgaccg gtcgtcgcct gcaatcgtgc
    14221 ctgttgcagt gcctgacgac catccggcgt ggaaagtagc atcgtagtcc gcgacgattt
    14281 atacgcattt gaactcggcg ctaactgcga cagccgatcg agctgttttt gcgcgccatc
    14341 aatatcgccc tgacgtaaca aagaacggaa acgggcggca acgacgtccg ggttattcgg
    14401 atcaataagt tccagccgat ataacgactg ttgcaccaga tcttcacgat gggtcgcttc
    14461 gcctaaccga acttgctcca gcaactgttg ctgagcggtt ggtgctgcct cgaccatcgg
    14521 catgacggcc agaccgaggg aaagcgtgaa tatgtttagt gtgaatttgc gcattcctgg
    14581 ccccagtcag gtaataactc accttttgtc gagaagcgga aacggtgttg atcccagcct
    14641 tgtccaaaca gggtcagcac atagttgtaa taggcatcgc tgctgggaaa gttatcggcc
    14701 acgcgctggc gctgaacggc ctgcgcatcg cggttttgta aaaagggcag catggcggca
    14761 gaaaaaccga ccggtccttt accctgcgct ttccccgtag ccacatccac tttttccggc
    14821 ggataaccgt ttttctcagt gaatgtcgcc atcggtttaa accggttgag catccgcgct
    14881 ttttgcggat cgctgtcagg catcatgcct acccacatgt aaacgcggat agcgtcgtag
    14941 ctgctgatca atgttttttc ggcttttagc tgccagcctt tgtctttctc atagcgcacc
    15001 cagtctggcg aaaaaccttt cggggcggtt tccagcaata aacgttgatt ggtttcgcgc
    15061 agcgtggtcc acggcgcgcc aaagcgggtg aaatactgcg ccagcgtcgg cggcaggtag
    15121 ctggggttaa aacgccagct gttatcctca gcaaaaccca ctttgcccgg taacaacatg
    15181 gaacccagcc caggcaccgt caccacttcc tcccgcgcga tacgttttag caacgcgctg
    15241 ccgatgtcgg tataacgctg ctctttccac aaacgccccg cctccagcaa cgaccaggcc
    15301 atccagacat caccatcgga ggccgaattg ctgtccagca cttcccactt actgttctct
    15361 ttcttgcccc acagccaggc gggcaaacgt tcttttaaag aaccctgagc gagattgttc
    15421 tgcgtccagt cgagaatatt atcgaaagct gcacggtcgt tagccgccag ggcaaagaac
    15481 atgccgtaac tttgcccttc ggaggtggtg attttgcgcg cgtcgctggg gtcgatgacg
    15541 cgcccttcct gactgatgta atccttttta aactgctccc aggcaggcca ggtacaggct
    15601 gcctgaacac taaaggcagc cagcagcagc atcgtcacga ttccactacg caacacattc
    15661 atcttcaatt actcgttatc cgggttaaga cgacgacgac taataattcg cagcagacgc
    15721 cacagtaccc atgccagcaa tatcacactg atagccgcca gcaccgccag cagaatcgga
    15781 tggtttgcca gcgcatacca cacgcgctcg aaccacggca gatgacctac gtaataaacg
    15841 tcgccaacac gtaggctgtt gataccggac tcgcggatca ccgcgaccga accgaacatg
    15901 gtggcgcgtt tgccgctatc gttcaccgca tcgttaagca tttcataacc gcgtgggcta
    15961 tctgccaaca gcgcaatcac gctgcgctgg tcgttatacg gcgactggaa gccaatcacc
    16021 gccgccatcg caccggaaga ggtcagcgtt gaccgggttt ctgccgcgcg atcgctctcg
    16081 tccggcacaa tgccggggaa cggggtctgg cgcatcggtg ttttcaccca gctttcggtc
    16141 gcctgcacca ataggtcgat ctgcttatcg tctttcagtt tgtccgggat accaccgatg
    16201 atcatgatgt cggcatcttt gccctgaatg gtgctgccat catcggtcac cgtcagatta
    16261 atcgccggga agcccgtctg tgcgccgata aaaccaacag tattcagcaa cgtttccatc
    16321 tgtgcttcgt taggcgcttt cggcatcacg gtgatggttt gcgacagatc cgccatccgg
    16381 ctgaatggga agcccgcgtt agcaaaggcg cgtagatccg gcatcgggat gaagtggtaa
    16441 tacttcgaga agtcgatggt ggagtcgtca ccaatcacca catgattctg caccggctgg
    16501 aaggtaatac agttatccac cgaaccgccc ggcatcgggt tcatatactc aaagtcgaag
    16561 cgcagctggt tggtcgcgcc cagtttcagc gccggaatag agacatctgt tttgccatcc
    16621 agcaaacctt gtaataccgg aatccgcagc agcaggcggt tcgcctcctg tttgctgctc
    16681 aggttgaagg attgcaggaa ctggttattc aggctgatat ccatccgcga actgtctttc
    16741 accggcggca tggtgtagcg gtaattaata tccatatcaa tgccggtact gcgcatcagg
    16801 tagagatccg gcggcaggtt tagcgaaacg ttaatcgctg ctggctcaag accgctggat
    16861 tgtaactgtt cttcataggt tttcagttcg ccaaatgtga ccggacgatc ggtacgtacc
    16921 cagttcggcg catcgtacgg cttacgcggt agcagcggtt tcacttcatt cactaccacg
    16981 ctttcaccac ggaacagaat gttaccctga gcgatacctt tcgctgcctg caacaggtct
    17041 ttgtcgtcac gaccaaacac caccagcagt ttgacgtaag gattctgcgg atggttaatc
    17101 atctcaatca caggggcttt taccgccgga tgatcgcgca ggaagtccgg ccgtttgtcg
    17161 ttggttgcaa agacaatggc attacgatcc ggcagttggt tatagagcac cgggaagttc
    17221 tgcccacgcc agccagaacg cgaaccaaac cacgaggcga caatggcaga ggcttgttgc
    17281 agcccaacat ccggcgcacc cgcaaagacc atcggcaagg tgttagtacg gttatcgcgc
    17341 gggtcaaaga atggcaccgg gaagtgtgac aggtcattct tcacattcag ggtctgatag
    17401 gtcagatcca gtccactgct ccgcccaaca tccagccaaa gcgtggtgct ggccgggttt
    17461 tcgcacacgt cctgataatg gccgacaaac tccagccgca cacggttgaa gtcggtaata
    17521 aacagtgggt taatgggcat ttgcgccagc gtttttttac ccaactgttc tttggtcact
    17581 ggcagcacgc ccatcagctc atcattgaga taaaccttta actgcgactg gacaggcagt
    17641 aacgatggcg atggcgtgta ttcgaggttg agcatcgcct tcgtcaccac ttcatcgctg
    17701 cgcataccaa actcaatgct gccgttcggg ttaatgccac gtagcaccat gctgcccggc
    17761 ggcggcgcaa tttgcgcaaa ggtcagcttc acatcacgcg aaggaccgtt ctgcgccacg
    17821 actggcgcat cagcgccctg cacgcccggc atcacttgcc ccacctgcgg attttgttcc
    17881 gtctgggcgg ctacagctgg ctcagcattg atcagtggtt gcgttgctgg cgtcgcctgc
    17941 gtcatgaaag aggggaacgc actcatcccc atagccactg cacaaatcca gaatagtttt
    18001 cttttcatcg cgttatcatc attgttgagc caaagcctga tccgatggtt gtgccgtttc
    18061 gctccgctcc gggcggcgcg gaataaacga tacaacccag gaaaccagag aagtcagcac
    18121 acggaatatg cccttcaccg aagaaggcgc aaactccgcc agatggcggt agccacggaa
    18181 gccgagcttc agaatatcca gcagactttc cagcggctta tcttccgggt agctgtcctg
    18241 ccagagcgcc catgtatccg cacgggcaaa cgtacactgc acaaaatcga tatgttgctg
    18301 ggtggtgagc ggcattaatt tcagcccaac ttcattaccc atcacgcgcg ccacctgggt
    18361 cgggaagacg tattcctgct gaccgcgttt aagcaacaga ttcactttct gcccttccag
    18421 aatctgcgcc tgaccgttga tcttgatccc caaaccaccg tcggagaaat cctgaacggt
    18481 acacgagaag aggtgaccat cttcgcgggc aattgccgcg ggcatcgtca tctccacgcg
    18541 gtgcgatcgg cgtacctgtt tgctttctac cgataccgca actgcgccgc caagaacaat
    18601 caggttgtag aacacccaca ccatactgac gaccacggtg agcatctcgg ttggcgggcc
    18661 atagaagtag cgccagatgc ctaccgcaac gcccaccagg ttgagtagaa caaggaagat
    18721 gtagggccgc gagatcaccc agtcgacgta ctcttcttcc accagtccac ctttggcggt
    18781 gacgttaaat ttgcctttgt gcgggttaat cagcgccacc agcgtcggtg gtgcgatata
    18841 ccacgccagc accgtttcgt agatttcact ccagaaagag tggcgatatt tgccctggat
    18901 cttcgagttg gtcaggctgg catggatcat atgcggcagc acgaacaggg cgatcatcaa
    18961 cgctggcgca tagatgatgt aggcatgaag cagcaggaac gccagcggcg cagtcaggaa
    19021 gatcagtcgt ggaatgcccg acaagaagtg gaacatggcg ttgacgtagc acagccgctg
    19081 ggcgaatttc agccctttac cggtaagcgg gttatcgagt gtaaccaggt gaatcatttg
    19141 ataccgacga gggttttagt gaaaggaggt aaaatcacct gtaagatact gaaataaaac
    19201 aggtgatgtc cggtgaacgc gaaaagtcta tttgataccg attgtgcaat aattagtcgt
    19261 ttatcctaaa atcggcaaca cttttgatac tacagattga cgcttaaaat tgctttaaaa
    19321 aaacttaaaa cgcttttctc cagtccgtat tgaaaggagt gtgccacagc acaaaatttg
    19381 cactactccc ttacgcaccg gcctttttct gggttacatt taaatgatta accttagcct
    19441 cacattcttc ataaatctct cttacagtat catcgggtaa ctcttgcaga ctcaatgcca
    19501 atgctctttt gaccccactc aatgagtgca gcttttgaat atcagtatca agtaaaatgg
    19561 ttagtaactc ggcccctttt ctgatcaata aattggacaa aatctcttgt tcagatgaag
    19621 ttagcgagtc ccacgccctc atgatgatgg agtcaccatc atttacatct tgtttttttg
    19681 ttctttctat ttgttctaat agttcttttt ttgtgattga ctcattatta taaccataga
    19741 ttagccactc cattgataca tgttcttttt cagcaatgtt ctggagatgg tgctttcttg
    19801 gctgtggtgg tggctgattg tttctccttt tgtaatagtt ctgcaatgtc tgaatattga
    19861 ttccccaggc cctagccgcc tcacttctcg atttgtaacg ctctgcaagg cgaaatattc
    19921 gctcgattac tggttctttt ccttcgtcaa gaaaagaact ttttttcatg ttcatgcctt
    19981 taggttcctt cgcgcttatc taattgattt tattgatgaa aacaatataa agaacaaaaa
    20041 gaacaaaata ccttgatttg gttcttttct tgttctatcg tttacccgta cagatatccg
    20101 tccgaatatc cgtgcgggta actttttaga atagaggaag tatgagtaga agagaagttt
    20161 cagatcacga ttggccgcca gaaatcatca aggcgcgcct gcacatggct ggcttatcac
    20221 tgcgctcttt atctttaaag gctggttaca gcagggattc gctgaaaagc gtattacgca
    20281 ccccttgccg cccatatcaa caaataattg ctgatgctct tggtgtgtca cctgaagaaa
    20341 tctggcccag cagataccag gttaagagct atatgagaaa ggcgtcatga tatgtatttc
    20401 attgcaaaag aactgattgg tgcgcccgga atgcctgcaa ctacaaaagg tattcgccag
    20461 gcattacaac gttacgcaca agggaaaagc tgttgttccc gtcgtcgctc aggatctaaa
    20521 gcaactgaat acagcatcga ctgtttacct gaagtgacgc agcaggcatt acgtgaacgc
    20581 tacgccctgc aactgatggc gcaaaaagcc gatgagtcac cggttccggt ggtgacaaag
    20641 gccagacgct cacctgccgt ggttgatgcg gtggaggcat atcgcggatc accccaactg
    20701 atggtcgaac gtatcaatgc actgactgaa aacagcgcca ggtggccgat gcacgaattg
    20761 cgattgtcag cgaggtgctt aaattcgcgc aacaacccgg tttcagctgc gctaaagcta
    20821 tccggtttat tgttgaccgt ctgtcacgct cgcaactgga cgaacgcatt gtggcaatgg
    20881 ttgaaacggc gaacgccaaa aagggaaaca gccgcgcgtt gagtgaaatc acgctgaaac
    20941 gctggattgc ggcctttaac aaggcgcaga acgccgctga acgcctactt ttactggcac
    21001 cgggtaaacg tcaggaaata aaagccgaag atattagctg gttgcccgag tttctggcgc
    21061 agtatcgcca gtcaaacggc cgaccaatga ccgaggctta cgaggatttt gtcgccgaat
    21121 ggcagcaccg gcacgctgat gagccttata tgctcgatat catgccctct tatgacacca
    21181 ttcgccgcgc gatgaagaaa ctgccggaag tggtgaaaca aaaaggccgg gtgaccggca
    21241 gtgaataccg ccagcttgag ggattcacgc gccgcgactg gtccagaatg ccggtgaatt
    21301 atgtctggat tggtgacggt cacggcatga agctgaaatg tgcgcacccg gttcacgggc
    21361 gaccatttgc accggaagtg acctttgtta tcgacggtgg cacgcgcttt gtggtgggct
    21421 ggagccttga cctggctgaa aatgttttcg ccgtagccgg tgccatacag cacggcattc
    21481 gccatcacgg caaaccgttt ctgtattact cggataatgg ctcgggggaa acggccgaca
    21541 tcctggataa ggaggttgtg gggatactac cgcgactggg gattaatcac ccgaccggga
    21601 ttgccggtaa tccgcaggga cggggcatta tcgaacggct taaccgcaca ttaccgatgc
    21661 gcatagcccg taaataccgc acctatttcg ggaaaggtgc agatcgcgag acgttacgta
    21721 aaaccaaccg cgatttacgc tcggcattca gtgccctgca acagggcaag cggctgaacg
    21781 cccggcagca gtcagcgatg cgtgatttac cgtcctggtc tgaactgatt gatgccattc
    21841 gtgacggcgt tgagtggtac aacaaccggc cgcacgatga attacccata aagccgaacg
    21901 gtaaacatta cagcccggcg gagttcagaa aaaaacgcct ggcggaagag gacacggaaa
    21961 ttgaatggct gtccgatgtg gaattgcggg acatgttccg gccgatggtg gaacgccctg
    22021 taagacgctg tgaaatacgc tggctgaata atatttacta cgcgcccgag ctgcgtgatg
    22081 aacatggccg caaagtgctt atcagctatg acattcatga tgccgaacga attaccgtac
    22141 gccgcccgga tggcagcgtg atttgcgagg cggtatggga cggcaataaa cgcgaagcct
    22201 tccctgtcag cgcggaatac tacaaacagc agcagcgcct taaaggtatg cgtaaacgcg
    22261 cagaggaaaa aatccgtgat gccgaggatg aggttgtcaa cgtgctggag cacaagccgc
    22321 aggagccatg gctggaaaac atataccgcc ctgtgggtaa tacggtgacc gttcagcaac
    22381 cagtcgctga cgatgaacct gacgaagaat acgagcgtaa tttccagcgg ggtttgcaac
    22441 tgctcgaagc gaaattaaaa gaaaatgatc cgctggcctg aaataaaaaa aataacccga
    22501 ggggtgactc aggttatttg aggtttctgt ctcggagaat gctgtctcaa gcgaggtgaa
    22561 taatatgacc gatattaacg atgtaatcaa gaccattgat gaacttattg atggcggcgt
    22621 actgacgcag tatgccatcg ccagagaggc gggaatttcc gacggcacat tatcggcttt
    22681 ccgtaagggg aaatataaag gcgataacgc tgctgtggct gcttccctgc gttcctggta
    22741 tgagaactgg aataaacaaa gcgcactgcc cgagccgccg cagtttgttg aaacgcagac
    22801 agtccaggag ctgcgcgcac tgtttcaggc ggttcgcctg atgggctgta ttaacgtcat
    22861 tgtgggcgta ccgggtgtgg gtaaaacggc caccgcccgt aattactgtc aggagcaacc
    22921 gaacacctgg atgatcaccc tgtcgcctgc gcattccagc gtcacggagt gtctgctgga
    22981 gctggcagag gaactgggga ttgattacac ccgcgcgaac aaaggggcat tatcccgcgc
    23041 aatccgccgt cgcctgatgg gaacgcgtgg actggtgatt gttgatgagg cggatcatct
    23101 tggtattgac ggtctggagc aactcagggc aatccaggac gccacgggga tcgggatggt
    23161 gcttattggt aacccgcgcg gactgtttaa aggtggacgc cgcgccgttg atgatttatc
    23221 gcgcctgttc agccgtcttg cccgtacaaa acaacttcgc aaggccaaaa aggcggatgt
    23281 gctggccatt gccagggcat gggggatcag tggtgaggcc gagctggccg tcatgcaggc
    23341 tatcgctgaa aaaccgggag cattacgtgt tctgacgcat acgcttaacc aggcgtggat
    23401 caccgccagt ggtgaaggcg ctgtgctgac agaaaaacat attaatgcgg cctttaaaga
    23461 ggtttatacc aaccctgaat tactctcaca ggtgtgatta tggcggtatt taatattccg
    23521 gatatttacg gacgctttta cctggtgaat ttcgataacg tgaaggtgat ttcactggcc
    23581 gaaaataaag aatgtggtga tttacttttt gaatttaatg accgcacacg aatggtgata
    23641 tctgccggac ttgatcgcga aggtgcgaca gaagtttaca ggggaatatg ccgttctgtt
    23701 ggtgcaaaac aagtcagata aacgaggtgt tatatgaata tgcaatcctg cggtaaaaaa
    23761 atgaatttat tcgactccct gaacagtgca cgccgtctga ctgaacttgc cggtgcggta
    23821 ctggaacgca gcaaacgcta cccacaacgt tatgccctga aaaccacgcc gccggtaagc
    23881 gaagttaagg gaaccggtga aattgaaatc accatacaga ccaacggcct gcgccgccgt
    23941 gtgaaggcta cccgcatcag cggctgcacg gtttactggg aggagtgagg tgaaacgaaa
    24001 tctaattgcg tgggcgtggt cgagcggtct cattgaactg ggttacgtcc tgccggaagg
    24061 tgcattgccg atagttgccg gaaagcctgc cacagtacgg catgtgattg aggttatggc
    24121 gcgtcatgga cgtgatgaac aggagcagtt actggttccg gggataccgg aagcggtgac
    24181 ggaggaagaa gcctttaatg ccatgattcg gttctgccgt gaggtcagac gccgggtcag
    24241 ttatccaaac agaacgagga ccagaggatg agtaaagtcg tacgcattat tttcgaatac
    24301 aaggagcacg ttatccataa aaacgcggat ggaacagtgc gcatggggat aagtctggac
    24361 atacgttcaa ccgggataaa gcagaaaggt gatggacctg ccatgatttt tggggtggtt
    24421 atgctcgcgg aaagcagaga ctttgctgaa cttgtggcaa tgaaagccag tgcgctcatg
    24481 aaagatatga gcatgcgttc cggggttatt aaaggtaatg aatttaatca
//
LOCUS       DAZGVQ010000071        24314 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000071 DAZGVQ010000000
VERSION     DAZGVQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.071"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..305)
                     /locus_tag="ACHLAG_002705"
     CDS             complement(<1..305)
                     /locus_tag="ACHLAG_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFP0655905.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLAALCLGLSHSTERV
                     SWIGNQMLAERFPDSSRLLLEDWERYLGLPECDMTGATIQERQRYAGNKYRMKPSLNR
                     "
     gene            complement(296..1378)
                     /locus_tag="ACHLAG_002706"
     CDS             complement(296..1378)
                     /locus_tag="ACHLAG_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFP0655906.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYALAG
                     LSAQEHEHLAWISRQIIPTEADEAELLKHCAFWGVIRKPASRADGPVQLMLTTDAGIT
                     EGVLLQRSDGVVYRITGSATGKAGTLNVNVEAESAGRAGNAPPGTKLTFITPQAGINQ
                     TTTVTGTGLTGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCRP
                     EWPQAGSVGVTFVQDNNPDIFPGEGDVKRVADYIRSHDDPATGQPVGQPLGPTISVFK
                     LTNKPVAFEIRIVPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKGLED
                     FDVRSPLKSVMAGDTELLTVGEITWL"
     gene            complement(1378..1815)
                     /locus_tag="ACHLAG_002707"
     CDS             complement(1378..1815)
                     /locus_tag="ACHLAG_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFP0655907.1"
                     /translation="MADIAVVWDQGCGSLQLNGADLLTDNSLLTAVIISLFTDRRALD
                     SDEIPDGTRDRRGWWGDSFRERPIGSRLWLLSREKTLSSVVSRAQAYADEALAWLHKS
                     GAATSVVCHAMRVGHDRLSLSVKITLPDGSRHPMIFYADMKGE"
     gene            complement(1808..2422)
                     /locus_tag="ACHLAG_002708"
     CDS             complement(1808..2422)
                     /locus_tag="ACHLAG_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFP0655908.1"
                     /translation="MMNDEVFSRLIAPVTRGIRLLFGRGVLTGTHDELKMQNVQLTGM
                     DGETFDDVERPQQYGQISVPLPGAETFFACLGGQRDQTVVLVVEDRRSRPTGLKAGDT
                     GVYHHEGHRIRLTKDGRIIVTCKMLEIYADEGMRVDTPEATFTGNVTVDKNLHVKGNV
                     SIDGTGRSQGTFTMSEAVIAGITYSGHVHHDNGEGSKTGGPENG"
     gene            complement(2412..3536)
                     /locus_tag="ACHLAG_002709"
     CDS             complement(2412..3536)
                     /locus_tag="ACHLAG_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFP0655909.1"
                     /translation="MSSRIELYIGGSIFSGWLTVSVRRSLEHLAGSFELGLMLPGERI
                     PSALRTGQSLTLRINGQTVISGWLDQVSQRISATRHQISISGRDKTGDLVDCAAIHPG
                     SQWRNRTLAQIAADLCAPFGIAVRWQVNDDTAARPFSSFTLENSETVADALTRSARHR
                     GVLVTSNADGDLVFTQAGSQQTDRLVLGDNLLDADYNTDWRGRYSEYRVRGHGRGGGK
                     RGDSESAARLAAPVGVISDEQIGRYRPKIILADQQTDTTGARQRALREMRRAIARSER
                     FSATVRGWFRDDGRLWDVNLLTGVSAQRFGIEQTELLVCQVEFLLDEQNGEVTRLVLA
                     PRDGFIVPAEPDSKGRGGSGDDVDAFIRQQMKKQGISFDE"
     gene            complement(3520..4878)
                     /locus_tag="ACHLAG_002710"
     CDS             complement(3520..4878)
                     /locus_tag="ACHLAG_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HFP0655910.1"
                     /translation="MASTDTPGRGSFRGVPFLVYQEQRERGGRNIVRREYPLRESGGA
                     DDLGPKLPEFTFTVLVTGDDLQTQRSRLRDALRAPGAGELMHPDYGTLNVLINSFESR
                     YNASEQGTVEFTINVIPASDDTAPSVVEDTAAALEQKSGSAMNQLFNTLSDGWTVISD
                     GLHDVQAMTDTISDKIDALENAVSGMGIVQDVSAFTASFTAIKGNAAALINAPRRMAE
                     SLAGIFAVLTGLPGNPSLSLTGKAGRPSGSLTTNRDSMTEQAMPQLYRTLSSLRYTLS
                     EQDDPQRLIGLTPAAQKNIRLLRSVMQSATLVSQAQTVGKLLDQVIRQNTTHVQDTDR
                     AGGLAWLESSADVQRINRDLSDAMEQQVLDLSAQGHTGTALALRDAGLALTEDLTTRS
                     VHLPGAFQVMVRTTEPALVTLYRATGNSRRWQYFVRRNNIPDPVFVPGGRSVEVISEQ
                     QD"
     gene            complement(4865..6922)
                     /locus_tag="ACHLAG_002711"
     CDS             complement(4865..6922)
                     /locus_tag="ACHLAG_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFP0655911.1"
                     /translation="MAKNLKASLIVDLLGNISAKSRQWSQELGAFSRSGRTGLGGLGN
                     AARRAGQETELVGSRMQRTLAGVRGSIRTVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNKAFIRPAAEMENYILRLNAINHGDKAKTEAVKAWAVQNAKDTTWGLAGVMQ
                     EYASSRGFGMSDREARRFITMLQDQGGYHGWSLSDAQGASLQLKQMFARQSIQAADAN
                     ILTGYGINVYQLLADKLGVNQKLIREKGEKGKLGPDSIRLLFQVMAEQAKGAQKNAMN
                     SWTGMTSMMGDVWDQFAREVMAKGPFDSLKKSLKGFLDYADAAQKSGLQDKLATQTAS
                     ALNQGFEYARDAATGFYRAIQKVRETLQALRDAGYGDALDRIGQGAQTAAKYLMYMYL
                     ASRALKVLRFAGAGALRLGATPLRYGMAMTSVLTSPFRKPQTTVPGTQPGRAGRFLNF
                     LTGVNPAAVQPVLVTNWPAGGLASGGGDVVVSGDGKTVRDRKKRGPGRGRGVTTVVTA
                     GEQLAESAGKQGFFGRMMSRAGGLLSAAGNRMGLGRFAGLFRGAGRLGGGALWAGAMA
                     APVLLDSSASAADKAGAVGSLAGSIAGGALGAAAGPVGVAIGSTVGSYLGDYLGGWLT
                     QAWQKLRGSSDENGGQATAKTAARVELVAPEGWRARSIDVDDTAQHGLDVNVWNGGNY
                     GLY"
     gene            complement(7050..7526)
                     /locus_tag="ACHLAG_002712"
     CDS             complement(7050..7526)
                     /locus_tag="ACHLAG_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFP0655912.1"
                     /translation="MTTRKKKTAVSDEAVMEAIRDALEDGDPRTAGLAEQLVNGYVDL
                     LDGLPFGESREYRVTFRELTAKDSIDAESEAERLMETRNGPVLVASPALRGIALLRRQ
                     IAAVGQIEGPLSPRQIGQLSERDLSRLMAAVSLLDSAMAGKLVAERGRPDAVPGSD"
     gene            complement(7541..7906)
                     /locus_tag="ACHLAG_002713"
     CDS             complement(7541..7906)
                     /locus_tag="ACHLAG_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFP0655913.1"
                     /translation="MSGKQYQGTATIRVNGQEYATLEGATFSPSGFEREVVKGAKVYG
                     YREKPREATLDCKFPAGGEGSPAADEINTWTAVTIEFVADTGEVHMMTKAWSSEPASI
                     DGGGDISAKFASATSTRVQ"
     gene            complement(7915..9417)
                     /locus_tag="ACHLAG_002714"
     CDS             complement(7915..9417)
                     /locus_tag="ACHLAG_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFP0655914.1"
                     /translation="MINFDGIGNDRRIPLIGVEFNNSMAVTGTPAQRQCVLLFGQARM
                     KENAVDGTGVLDVPVRITRASQAVELWGRGSMIALMVAEFIAINPDAELYAIAQGAGT
                     GGANAAAFNLTGTAATDGLLCIYIGGRRYALPVKAGQKGKAIIDALVALINKDTDAPF
                     TAVSAVVDGSNAGGLKGSMGVSARFIGECSVHDLRLNYHDGETLPSGLTPQTGFPSSR
                     ARNPDITRSVAGMGDRQYNYVVMPYKDDANLKIISDELLKRWGPAKMSDGVLWLAHTG
                     TFGEVQAFGAKRNDFLATCTSIPKAPEPDYLWAAAVCATCAPSLGADPARPLQTLALP
                     SRMAPAPADRLTREERNSLLWGGIATVSVAAGDMVQIERQVTMYRQNVYGESDPSYLD
                     VETIYTLSYLRYSLRTFITQRFPRHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWV
                     ELGLVENLDTFKKNLLVERNTSDRNRLDVLCTPDLVNQFRFLAAQIRFIL"
     gene            complement(9414..9659)
                     /locus_tag="ACHLAG_002715"
     CDS             complement(9414..9659)
                     /locus_tag="ACHLAG_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFP0655915.1"
                     /translation="MKTIFIKPAPGRLIRDPDTMRPLAQEGEEKTFTPFWCRRLDDGD
                     VIQAEKAAEEAPAVSADATTATEKPVAQPASDKEKPQ"
     gene            complement(9660..10220)
                     /locus_tag="ACHLAG_002716"
     CDS             complement(9660..10220)
                     /locus_tag="ACHLAG_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFP0655916.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREASRPGLYQIVARLIAVLNGFRTE
                     KTSPLYFEKAVNGYTETQANSGAVMYALYFSCEEMIAPLTDISSLDDFLRHYETFVEP
                     PGTPPFEAHIDLPGNTTTAVEPPEEL"
     gene            complement(10217..10636)
                     /locus_tag="ACHLAG_002717"
     CDS             complement(10217..10636)
                     /locus_tag="ACHLAG_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFP0655917.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSARYTLPLEVIPAVLVQHCCAIAFYYLCDQRASDQARDRYSEALAWLKDVMN
                     GNVPVGVDTNGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            complement(10633..11022)
                     /locus_tag="ACHLAG_002718"
     CDS             complement(10633..11022)
                     /locus_tag="ACHLAG_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFP0655918.1"
                     /translation="MNEHHTVAATDDSGLQVSGDNSVTVLAEVRCSRSAFRRAGFLFT
                     RGRQQVEVTPEQLARLEAEPCLTVRILQTPADDAGSVAGVVHAVAGTDLAEAEAEAEA
                     EAEAEAGQDAPRKKTGNKAKQAKKAHA"
     gene            complement(11066..12013)
                     /locus_tag="ACHLAG_002719"
     CDS             complement(11066..12013)
                     /locus_tag="ACHLAG_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFP0655919.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYKKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAINRDDFEDDNLGIYSP
                     LFQEMGRAAAAQPDELVFAALRDGINAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAIKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMREFTADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
     gene            complement(12013..13137)
                     /locus_tag="ACHLAG_002720"
     CDS             complement(12013..13137)
                     /locus_tag="ACHLAG_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFP0655920.1"
                     /translation="MKTKNTPKLAYAILNAISLSADGDGDWCQIMPAGRVKARDGRPE
                     KPAEGWLINRATVERMVSRVVALNQPVKIDYNHQTLIKGHQAPAAGFVTASPENFRFS
                     EERGFEVRPKWNPPALEHLRNNEFPWFSPVIGYDENTGEPVELRMLAITGDPGLTGMN
                     PVAALSADDLYNALNPPLKDTSMNEQLRQLLTALGLTVADGDEFTPELGTAALSALTG
                     IKTRADAHDNLKTQVASLSAELETAKGTPAGGSIDLTKYVPVETYNALRTEYVALSAQ
                     HGSTTLEQVLDKAESEGRIFKSERGYLEQLGGQIGVAALSAQLDARQPVAALTGLQTD
                     TVTVPDKKTATAVLSAEDIAAAKLLGKTEAEFLKMKEEMQ"
     gene            complement(13314..13787)
                     /locus_tag="ACHLAG_002721"
     CDS             complement(13314..13787)
                     /locus_tag="ACHLAG_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFP0655921.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVASSLLSSSE
                     MAFEQEKEPDGERWHDWSDPYRKWRTRKGYMPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMAEIRKRFKKATETP"
     gene            13826..13913
                     /locus_tag="ACHLAG_002722"
     tRNA            13826..13913
                     /locus_tag="ACHLAG_002722"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            complement(13913..15238)
                     /locus_tag="ACHLAG_002723"
     CDS             complement(13913..15238)
                     /locus_tag="ACHLAG_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFP0655922.1"
                     /translation="MAQGIDLGYAATLPSKEAVAYFRAKGVHISWNWFETDADVHARS
                     FTAAKVARLDVLTTLQVEVQRAVDEGISQKAFIRTLTPRLQKLGWWGKQIVVDSAGNA
                     EEVQLGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPAHSAL
                     NGLVFRYDDPFWNTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGQDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGTRTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMKTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSVKHQNDGIALTPEDFGRLPAMLAAPDAVLWDNVHQNLLYITETRDG
                     TAKIAVNAPYSVKRQPDKLDVVINAYRVSRNTLIDAVQGGKMEILEGKL"
     gene            complement(15222..16811)
                     /locus_tag="ACHLAG_002724"
     CDS             complement(15222..16811)
                     /locus_tag="ACHLAG_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HFP0655923.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSTALDIPQIASRYVEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPPNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKITSAVMQLSTGMDIS
                     DPWIREQTGIPQPTPGEAIFRVRQSGNEPAQTDKEMPPEKQEKTEQTALAARLPEAKS
                     SPRDELDDMGDAVPARRLQEAIDPVLEPVIDAIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGTGD"
     gene            complement(16811..18475)
                     /locus_tag="ACHLAG_002725"
     CDS             complement(16811..18475)
                     /locus_tag="ACHLAG_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655924.1"
                     /translation="MNNAEELSPLLTNTVSTRKIDLAGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFEDESEVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAMALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTGREWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLLEALNPRSRFSFGEDFARTGDLSCFVLLEITESLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAANARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            complement(18475..19056)
                     /locus_tag="ACHLAG_002726"
     CDS             complement(18475..19056)
                     /locus_tag="ACHLAG_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFP0655925.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGMDGMSEQLESRIKGILLGKV"
     gene            complement(19059..19349)
                     /locus_tag="ACHLAG_002727"
     CDS             complement(19059..19349)
                     /locus_tag="ACHLAG_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFP0655926.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRVLINGYFIAELTGRGQDVAEGRASVPGVKKPRARG"
     gene            complement(19346..19654)
                     /locus_tag="ACHLAG_002728"
     CDS             complement(19346..19654)
                     /locus_tag="ACHLAG_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFP0655927.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VEKVETRLSSLPTEAEVNRLNVEIVTLRGELKTTNATLRSVSYQNELLLEQAVRKKTQ
                     "
     gene            complement(19635..19862)
                     /locus_tag="ACHLAG_002729"
     CDS             complement(19635..19862)
                     /locus_tag="ACHLAG_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP0655928.1"
                     /translation="MDDSDRAQAVMERGSERALCNRLTRKCRAMDAPGRRVCADCGGE
                     IPAARLVAVPDAIRCVNCQNIMEARHVGQHR"
     gene            complement(19872..20090)
                     /locus_tag="ACHLAG_002730"
     CDS             complement(19872..20090)
                     /locus_tag="ACHLAG_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655929.1"
                     /translation="MPIPAEWLADCLVPPAPEPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLEARRQHEFTDMAGTPAVPAGKTK"
     gene            complement(20074..20424)
                     /locus_tag="ACHLAG_002731"
     CDS             complement(20074..20424)
                     /locus_tag="ACHLAG_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655930.1"
                     /translation="MAALGVMSVLAAVVWGMHQKTQRLEDNNHVLVRERDEARLVLAN
                     QQRTLQLISQISEAATNEKQQNIQHSEGQQSVVRRSLAAVPAASAPVPDDVADRVRRA
                     VCEIRACEAGADPR"
     gene            complement(20537..21037)
                     /locus_tag="ACHLAG_002732"
     CDS             complement(20537..21037)
                     /locus_tag="ACHLAG_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0655931.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENSTYIDP
                     TGTPTICYGHTGPDVKPGMTLTDEECLELLEKDMKWAFAAIDRRVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            complement(21109..21531)
                     /locus_tag="ACHLAG_002733"
     CDS             complement(21109..21531)
                     /locus_tag="ACHLAG_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFP0655932.1"
                     /translation="MAETQMSMFGDSEQLHALIDRLDDIPDDELKKNWPGTLRDLVDV
                     ISAELHRQGIEPAQAGMLARKVAAAQAGYMGGRGYYLPVGESLFTELRNNEIFSRWSQ
                     GEKIETLRRHYRMSETQIYTVIREQRRLHLARTQPPLF"
     gene            complement(21637..21873)
                     /locus_tag="ACHLAG_002734"
     CDS             complement(21637..21873)
                     /locus_tag="ACHLAG_002734"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655933.1"
                     /translation="MDIFLYSGGTLSGRLKLFVFALEQAWCALRVGIIGFHVDETTIT
                     RNMHKLFDKKNSFRVCIFKNKNDLYHIVTPENDQ"
     gene            21872..22294
                     /locus_tag="ACHLAG_002735"
     CDS             21872..22294
                     /locus_tag="ACHLAG_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655934.1"
                     /translation="MKQPPSLLLSCPPELAHELNDLAKEYKLTNHNIEAATLSAAGSD
                     IVLIVLAAASCDGFWQALASMFQTWLHRKPTKQTPPEKKVIRSVIVRSWEHYQETTKQ
                     TEFYNFDADELAKLIALYEKPDETIGCIIPSETEIQEK"
     gene            complement(22321..22830)
                     /locus_tag="ACHLAG_002736"
     CDS             complement(22321..22830)
                     /locus_tag="ACHLAG_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFP0655935.1"
                     /translation="MRGKLISAIHVAKRELALDDETYTSALLAATGKASCRDMSPDEL
                     SRVLDVFKKRGFKVRQKPVNRALKPGTVTAKIRAIWKVMHRQGFISDGAETALNRWVQ
                     SQTAAQNGGEGVANWQWLEQHPELASDVLERLKRWHRRKMLAALDMSERAVMSYERVC
                     RLYEKSFPC"
     gene            complement(22827..23138)
                     /locus_tag="ACHLAG_002737"
     CDS             complement(22827..23138)
                     /locus_tag="ACHLAG_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655936.1"
                     /translation="MDEQITDNISLLFWCLYITSFFGAFVITRWLCRKIISLFDKRYL
                     LELAADALIQQAIVLYSGEFFCRITTRDGWHIMIIPPTHHIRWNEPEKAFHVRKKVNT
                     V"
     gene            complement(23131..23463)
                     /locus_tag="ACHLAG_002738"
     CDS             complement(23131..23463)
                     /locus_tag="ACHLAG_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655937.1"
                     /translation="MITTLFVESDEPLVCAAGMPVCGGTLTGVYFGDLRGYPWHSLND
                     AFPPDMEAVVLIVQYGHRQELRIGHIGYEGFFVDEETGACLEDEDGQVTHWCHISALP
                     ELREIHNG"
     gene            complement(23460..24011)
                     /locus_tag="ACHLAG_002739"
     CDS             complement(23460..24011)
                     /locus_tag="ACHLAG_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsnC family protein"
                     /protein_id="HFP0655938.1"
                     /translation="MMILQPMGRKGQAPAHVRAWTPEEDALLIALYPSTPVKNIAVRV
                     KRSFWGVHNRIVLLRGTYPELLKCKRPRFKHDEDKFIRKNARTMTGKQIGEYLGRDRD
                     SVHNRARYIGVSMKKYGELLPFTRIPDDDVHLIRELRDAESPRRLTFREIGEKFELSE
                     STVNFIYHHRRTAGDAVLRELTP"
     gene            complement(24015..>24314)
                     /locus_tag="ACHLAG_002740"
     CDS             complement(24015..>24314)
                     /locus_tag="ACHLAG_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655939.1"
                     /translation="TASNGWSCTPRTSRIPKTLRVASDELNILWREYSPVTYARTDAL
                     LFWLGIDFSAIMFGPVKWFCVDDVIYLECGVKPANQKMTEILSDEFYAAEKRVRG"
BASE COUNT         5182 a         7311 c         6391 g         5430 t
ORIGIN      
        1 cggttaagag agggtttcat ccggtattta ttcccggcat aacgctgacg ctcctgaatg
       61 gttgcgccgg tcatatcaca ttccggtaac cccagataac gctcccagtc ttccagcagc
      121 agacgggatg aatcaggaaa acgttctgcc agcatctggt taccgatcca ggaaacacgc
      181 tccgtggaat gactgagacc caggcacaaa gccgcaagaa cggatgacgg ccgcttgtcc
      241 catgccagac cgtcaggcag caactgcagc agggcgcgct gatgcggggt cagggttaca
      301 gccatgtgat ttcccccacg gtcagcaact ctgtatctcc ggccatcacg gatttcagcg
      361 gacttcgaac gtcaaaatct tccagtcctt tcacccctgc aacagcccgc cagaatgatg
      421 aaggcagcac cagtccaccc ggccgcgatt cgttataaag caggtccgtt aatgcctgtt
      481 ttacggcagc ctgattttcc ggcgttttgg gtacaatcct gatctcaaac gccaccggct
      541 tattggtcag cttaaacacg ctgattgtcg gcccaagtgg ctgaccaacg ggctggcccg
      601 tcgccggatc atcatgactg cggatataat ccgccacccg cttcacatca ccttcaccgg
      661 ggaaaatgtc agggttgttg tcctgaacga acgttacacc cacgctgccc gcctgcggcc
      721 attccgggcg gcaccatgcc cgcgtcacgc ccggcacctc acgcgcccag cgttcaaaat
      781 cgtactgcgt gccaccggac ggcggattct gtacccggaa aaccaggcgg gaaagcagct
      841 ccggcacggt ttccacatcc gcaccaccgg tgagtcccgt gccggtcacc gtggtcgtct
      901 ggttgatccc cgcctgcggt gtgataaatg tcagttttgt ccctggcggg gcatttccgg
      961 cgcgccccgc actttccgcc tccacattaa cattcagtgt tccggctttt ccggtcgcag
     1021 agccggtgat gcggtacaca acaccatcgc ttcgctgaag gagtacacct tctgtgatcc
     1081 ctgcatccgt ggtcagcatc agttgtaccg gtccgtctgc tcgggaagcc ggtttacgga
     1141 tgacacccca gaatgcgcag tgtttcagga gttcggcttc atcagcttct gtcggaatta
     1201 tctgccggga gatccaggcc agatgttcat gctcctgtgc tgacagaccc gccagtgcat
     1261 acgcaatggc attcagggtg gtttcattca ctcccggctg cgaaccgggc aggcgctggc
     1321 tgatatcctg ttgtgtctgt gtgatcagtt gcgccagtgg taagggctga taaggcatca
     1381 ttcccccttc atatcagcat aaaaaatcat cggatgtctg cttccgtccg gcagggtgat
     1441 tttcacggaa agcgaaaggc gatcatgccc cacacgcatg gcatgacata ccacggatgt
     1501 ggcagcaccg cttttatgca gccacgccag cgcttcatca gcataggcct gtgcacggct
     1561 gaccacggag gacagcgtct tttcacggct taacagccag agacgggagc caatggggcg
     1621 ctcccggaaa ctgtctcccc accatccccg acggtcacgg gtgccgtcag ggatttcatc
     1681 ggaatccagc gcccgcctgt ccgtaaacag tgaaataatg accgcagtca gcaggctgtt
     1741 atccgtcaga agatctgcgc cgttcagttg cagggaacca caaccctgat cccagacgac
     1801 agcaatatca gccattctct ggtcctcccg ttttgctgcc ttcaccgtta tcgtgatgca
     1861 catgacctga ataggtgatc ccggcaataa ccgcttcgga catcgtgaac gtcccctgtg
     1921 atcttccggt gccgtcaatg gacacattgc ctttgacatg caggttttta tccaccgtca
     1981 cattgcccgt aaaggtggct tccggcgtat ccacccgcat cccctcgtcg gcgtaaatct
     2041 ccagcatttt gcatgtcaca ataatgcgac catcctttgt taaccgtatc cggtgcccct
     2101 catggtgata cacccccgta tctccggctt tgagtccggt cggacggctg cgccggtctt
     2161 ccaccacaag caccacagtc tgatcccgct gtccgcccag acaggcaaaa aaggtttctg
     2221 cacccggcag gggaacgctg atctgcccgt actgctgggg gcgctccaca tcatcaaagg
     2281 tttcgccgtc catgccggtg agctgcacat tctgcatttt cagttcgtca tgtgtgccgg
     2341 tcagaacgcc ccggccaaac agcaggcgaa taccgcgcgt taccggggca atcaggcggc
     2401 tgaacacttc atcattcatc aaagctgatc ccctgttttt tcatctgctg gcgaataaag
     2461 gcatcaacat cgtcaccgga accaccccgg cccttactgt ccggctctgc cggaacgata
     2521 aagccgtcac gcggtgccag taccagccgg gtgacttccc cgttctgttc atccagtaaa
     2581 aactccacct gacagaccag cagttcagtc tgttctatac cgaaacgctg ggccgaaaca
     2641 ccggtcagca gattgacatc ccataaccgg ccatcatccc ggaaccagcc acgcacggtg
     2701 gcagaaaacc gttctgaacg ggcaatcgca cggcgcattt cacgcagggc acgctgccgt
     2761 gcaccggtgg tgtctgtctg ttgatcggcg aggatgattt tcggccggta gcggccgatc
     2821 tgctcatcac tgatgacgcc cacaggtgct gccagccggg cggcggactc gctgtctccc
     2881 cgtttgccac caccgcgccc gtgtccccgg acacggtatt cactgtatcg tcctcgccag
     2941 tccgtgttgt aatcggcatc aagcaggtta tctcccagca ccagtctgtc cgtctgctgg
     3001 cttcctgcct gggtgaacac cagatcgccg tcagcattac tggtcaccag caccccgcga
     3061 tgccgggcag aacgggtcag cgcatccgcc acggtttctg agttttccag cgtaaaagag
     3121 ctgaaagggc gtgcagccgt gtcgtcgtta acctgccagc gcacagctat cccgaagggc
     3181 gcacataaat ctgcggcaat ctgtgccagc gtccggttgc gccactgact gccggggtgg
     3241 atggcggcac agtccaccag atcgccggtt ttatcccggc cgctgatgct gatctgatga
     3301 cgggttgcac tgatccgctg actgacctga tccagccagc cactgatgac cgtctgcccg
     3361 ttaattctga gcgtcaggga ctgaccggtc cgcagggctg aggggatgcg ctcaccgggc
     3421 agcatcagcc ccagttcaaa cgacccggcc agatgctcca gcgagcgacg gacgctgacc
     3481 gtcagccagc cggaaaaaat actgccgccg atatacagtt caatcctgct gctcactgat
     3541 cacctccacg ctgcggccac ccggaacaaa aaccggatcg gggatgttgt ttctgcggac
     3601 aaaatactgc cagcggcggc tgttgccggt ggcacggtac agcgtgacca gtgccggttc
     3661 ggtggttcgc accatgacct gaaaagcgcc gggaagatga acactccgcg tggttaaatc
     3721 ttctgtcagc gcaagccctg catcacgtaa tgcaagggcg gtgccggtat ggccctgtgc
     3781 ggacagatcc agtacctgtt gttccatggc atcactcaaa tcccggttga tacgctgcac
     3841 atcggcagaa ctttccagcc aggccagtcc acccgcacgg tcagtatcct gaacgtgcgt
     3901 tgtattctgc cggatgacct gatccagcag tttcccgacc gtctgcgcct gcgacaccag
     3961 ggttgcactc tgcatgacgc tccgcagcag acggatattt ttctgtgccg caggcgtcag
     4021 accaatcagg cgctgcggat catcctgttc gctgagggtg taacgcaggg aagaaagtgt
     4081 gcggtacagc tgcggcattg cctgctctgt catgctgtca cggtttgtgg tcaggctgcc
     4141 ggacggcctt cctgccttcc cggtcagtga cagggaagga ttgccgggca gcccggtcag
     4201 cacggcaaat attccggcca gcgattcagc catgcgacgg ggcgcattaa tcagggcggc
     4261 ggcattgcct ttaatggcgg taaaactggc cgtaaaggca ctgacatcct gcacaatccc
     4321 catccctgaa acggcattct ccagcgcatc aatcttatcg ctgatggtgt cggtcatggc
     4381 ctgaacatca tgcagaccgt ctgaaatcac cgtccagccg tctgaaagcg tgttaaacag
     4441 ctgattcatt gctgaaccgc ttttctgctc cagcgcagct gccgtatctt ctaccaccga
     4501 aggggcggta tcgtcactgg cgggtatcac gttgatcgtg aactcaaccg ttccctgctc
     4561 gctggcgtta taacggcttt caaagctgtt tatcagcacg tttaacgtgc cgtaatccgg
     4621 gtgcatcagt tcaccggcac cgggtgcacg cagcgcatca cgcaggcggc ttcgctgtgt
     4681 ctgaagatcg tctccggtca ccagaaccgt gaatgtgaac tccgggagtt ttggccccag
     4741 atcatccgca ccgccgcttt cacgcagtgg gtattcacgg cgcacaatgt tacgtccgcc
     4801 ccgttcccgc tgctcctgat aaaccaggaa cggtacgcca cgaaaggagc cacgccccgg
     4861 cgtgtcagta gaggccatag ttccctccgt tccagacgtt cacatccagg ccatgctgtg
     4921 cggtatcatc cacatcaata ctgcgcgccc gccagccttc aggggccaca agctctaccc
     4981 gtgcggcggt tttcgcggtg gcctgtccgc cgttttcgtc actgctgcca cgcagtttct
     5041 gccaggcctg cgtcagccag ccccccagat aatcaccgag atagctgccc actgtggaac
     5101 caatggcaac cccgaccgga ccggcagccg cccccagcgc gccaccggcg atacttccgg
     5161 caagggaacc cacggcaccg gctttatcgg ctgcactggc gctgctgtcc agcagtacag
     5221 gagccgccat ggcaccggcc cacagggcac ccccacccag ccgaccggca ccacggaaca
     5281 gcccggcaaa acgccccagc cccatgcggt ttccggccgc cgaaagcaga ccaccggcac
     5341 ggctcatcat acgaccgaag aagccctgtt taccggcgct ctccgccagc tgctcaccgg
     5401 cggtcacgac agtcgtgaca ccccgcccgc gtccggggcc gcgtttttta cggtcgcgca
     5461 cggttttgcc gtcaccgctg accaccacat ccccgccgcc tgaggccagg ccacccgcag
     5521 gccagttggt gaccagcacg ggctgaacgg cagcaggatt taccccggta aggaaattca
     5581 ggaagcggcc agcgcgtccg ggctgcgttc ccggcacagt ggtctgcggt ttgcggaagg
     5641 gcgacgtcag cacggaggtc atcgccatac catagcgtaa gggggttgcg ccgagacgta
     5701 atgctcccgc ccccgcaaac ctgagcacct tcagcgcgcg ggaggcgaga tacatataca
     5761 tcaggtattt tgcggcggtc tgcgccccct gaccaatacg atccagtgca tcgccataac
     5821 cggcgtcgcg cagtgcctga agcgtttccc tgactttctg aatggcacgg taaaagccgg
     5881 ttgcagcgtc tctggcatac tcgaatccct gattcagggc cgaggccgtc tgtgtggcca
     5941 gcttatcctg taagccgctt ttctgtgccg catcagcata atccaggaag cctttcaggc
     6001 tctttttcag actgtcaaac ggtccctttg ccatcacttc acgggcaaac tgatcccaga
     6061 cgtcgcccat catggacgtc attcccgtcc aggaattcat ggcgtttttc tgggcacctt
     6121 ttgcctgttc ggccatcacc tgaaataaca ggcgaatgct gtccggcccc agttttcctt
     6181 tttcgccttt ctcacggatg agcttctggt ttacgcccag cttatcggcc agtaactggt
     6241 aaacgttaat cccgtatccg gtcagaatat tggcgtccgc tgcctggata ctctggcggg
     6301 caaacatctg tttgagttgc agggacgcac cctgtgcatc tgacagtgac cagccgtgat
     6361 agccgccctg atcctgaagc atggtaataa agcgacgggc ttccctgtca ctcatgccaa
     6421 acccgcgact ggaggcgtat tcctgcataa cccccgccag cccccaggtg gtgtctttgg
     6481 cgttctgtac tgcccaggct ttcactgctt ccgtttttgc cttatcgcca tgattaatgg
     6541 cgttgagacg cagaatatag ttctccatct ctgcggccgg gcggataaag gctttattaa
     6601 aaccgtagac cgcagcccct cccgccagca tcccgtaaag gttgcttatc cgcccgatgg
     6661 tgccggtaat gctgccctgc aggcggtcaa aatctgacgt taccgtgcgg atgctgccac
     6721 gaacaccggc aagcgttcgc tgcatacggg agccgacaag ttctgtttcc tgaccggccc
     6781 ggcgggctgc atttccaaga ccacccaggc ctgtcctgcc ggaacgggag aacgccccca
     6841 gctcctgtga ccactggcgg gatttggcgg aaatattgcc gagtaaatcg acaatcaggg
     6901 aggctttcag gttttttgcc atgggttact gcttcgttct gagaattttt tcggtctgtc
     6961 ggcagtgccg gtaaagctgc gataagggga ggttaagcgc ccactccggg ccgctttttg
     7021 tcaccatccc cagcacaatt gcggcttctt caatctgatc ccggcactgc atctggtcgc
     7081 ccccgttcag cgaccagctt tcctgccatc gcggaatcaa gcaggcttac cgccgccatc
     7141 agacgggaga gatcgcgctc gctgagctgt ccgatctgac gcggagataa tggcccctca
     7201 atctggccga cagcggcaat ctgacggcgc agcagcgcaa tgccgcgcag tgccggagag
     7261 gccaccagaa ccggaccgtt tcgggtttcc ataagacgct cggcctcact ctccgcatca
     7321 atgctgtctt tcgccgtcag ttcgcggaag gtgacgcggt attcacggga ttcaccgaac
     7381 ggcaggccgt cgagcagatc cacatagccg ttcaccagct gttcagccag cccggcggtg
     7441 cggggatcgc cgtcctcaag tgcatcccgg atagcctcca tcacggcctc gtcagaaacc
     7501 gccgtttttt tcttacgtgt ggtcattgca tttttcctga ttactggaca cgggtactgg
     7561 tggcgctggc gaacttcgcg gagatatcac cgccaccgtc aatcgaggcc ggttcactgc
     7621 tccaggcctt cgtcatcatg tggacttcac cggtatcggc cacaaactca atcgtgaccg
     7681 cagtccaggt gttgatttca tcggcagccg gtgaaccttc tccgccagcc ggaaatttac
     7741 agtccagcgt cgcctcacgg ggtttctcgc gatagccgta gactttcgcg cctttcacca
     7801 cttcacgctc aaagccggac ggggagaacg tggctccctc aagggtggcg tactcctgtc
     7861 cgttcacacg gatggtggcc gtgccctgat actgttttcc gctcatgcct tacctcacaa
     7921 aatgaaacgg atctgtgctg ccaggaagcg gaactggtta accagatccg gcgtgcataa
     7981 cacgtcaagg cggtttcgat ctgacgtatt acgctccaca agcagattct ttttaaacgt
     8041 gtccagattt tcgaccagcc cgagttccac ccactcttca cccagcgaaa ttaactgaag
     8101 cttcatgatc tccggcgtca cgatattctg accggcacga acgggcgtcc cgtcatcagc
     8161 cagtttatgg cgcggaaaac gttgagtgat gaaagtgcgc agggaataac gcagatatga
     8221 cagggtgtag atggtttcca catccagata gctgggatca ctttcgccgt acacgttctg
     8281 gcgatacatc gtcacctgac gctcaatctg aaccatatcc ccggccgcca cactgaccgt
     8341 ggcaatccct ccccacagca gactgttacg ctcttcacgc gtcagacgat cggccggggc
     8401 cggggccatg cgtgacggaa gcgccagcgt ctgaagcgga cgcgcaggat ctgctcccag
     8461 agagggtgca caggtggcac agaccgccgc tgcccagagg taatccggtt caggggcttt
     8521 gggaatggat gtgcaggtcg ccagaaaatc attgcgtttt gccccgaatg cctgcacctc
     8581 accaaacgtg ccggtatgtg ccagccacag cacgccgtca gacattttgg ccggtcccca
     8641 gcgtttcaga agttcatcac tgatgatttt caggtttgca tcgtccttgt agggcatgac
     8701 aacatagttg tactggcgat cccccattcc ggccacactt cgggtgatat cgggattgcg
     8761 ggctcttgag gagggaaagc ctgtctgggg cgtcagccct gacggcaggg tttcaccgtc
     8821 gtgataattc aggcgcagat cgtgcacaga gcattcacca atgaagcggg cgctcactcc
     8881 catacttcct ttcagaccgc ctgcattact gccatcaaca accgcagata cagctgtgaa
     8941 tggcgcatca gtatccttat ttatcagggc aacaagcgca tcaatgatag cttttccttt
     9001 ttgtccggct tttacgggca gcgcatagcg tcgtccacca atatagatgc agagcaaacc
     9061 atcggtcgca gccgtaccgg taaggttaaa ggcggcggca tttgccccgc ctgttccggc
     9121 cccctgcgca atagcataca gttccgcatc aggattgatg gcaataaatt cagccaccat
     9181 cagagcgatc atggagccac gcccccagag ctccacagcc tgagaggcgc gagtgatcct
     9241 gaccggaaca tccagcacac cggtaccgtc cacagcattc tctttcattc tcgcctgacc
     9301 aaacagcagc acacactgac gctgtgccgg ggtgccggtg accgccatgg agttattaaa
     9361 ctcaacccca atcagcggta tacggcggtc attaccgatc ccgtcaaaat taatcattgc
     9421 ggtttctcct tatcggatgc cggttgggcg accggttttt ctgtcgcagt cgttgcatcc
     9481 gcagatacag ccggagcctc ttcagcggct ttttcagcct gaatgacatc gccgtcatca
     9541 agacgccgac accagaacgg cgtgaaggtt ttttcctccc cctcctgtgc cagcgggcgc
     9601 attgtgtcgg gatcacgaat caggcgtccc ggcgcgggtt tgataaagat ggttttcatt
     9661 tacagttcct ccggcggttc gaccgccgtt gtcgtgttcc ccggcaaatc aatatgtgcc
     9721 tcaaacggcg gcgtaccggg cggctccaca aaggtttcgt aatgacgcag gaagtcgtcc
     9781 agcgagctga tatccgtcag tggcgcgatc atttcctcgc aggagaaata aagcgcatac
     9841 atcacggccc cactgttcgc ctgggtttcg gtgtaaccgt tgaccgcctt ttcaaagtaa
     9901 agcggtgagg ttttttctgt tctgaagccg ttaagcacgg caatcagccg ggccacaatc
     9961 tgatacagtc cgggacggct ggcctcacgc ccgttgagca tgtcgccgat gacgtagaac
    10021 acccagtgac tgaccaggcg accacgggta cggccttcac cggcacccag ccaggcaacg
    10081 tagatcgccg gagcgttaat cagcatggtg cgcagtacgc tgtcgctcca gtcgccggga
    10141 tgcgtgtcaa cagacacaag ttcattcccg aaatactcac ggatacgggc gatgtatgcc
    10201 tgttcggttt ccgtaatcat atgaagccct tctggttgcg cccgaatacc gccgcatcag
    10261 actgaacctg tggtaaatcc ccggattcag gggccgcacc gttcgtatcc acaccgaccg
    10321 gcacattgcc gttcatgaca tctttcagcc aggccagcgc ttcgctgtaa cggtcacgcg
    10381 cctgatcgga ggctctctga tcgcacaggt aataaaaggc aatcgcgcaa cagtgctgaa
    10441 caagaactgc cggaataacc tccagcggca gcgtgtaacg ggcggacaga tagctgtcga
    10501 taagggctga cgcatctgtc agcgcccggt tcagcttgcg cgtgtccggc tcatccgaac
    10561 ggggcactgc cagtaacggc ctgagcaaat cctcgcggta acgcgcccgc atatcggttt
    10621 cagtggcgta attcatgcgt gggccttttt cgcctgtttc gctttgttac cggtcttttt
    10681 ccggggagca tcctgtccgg cttcggcttc ggcttcggct tcggcttcgg cttcggcttc
    10741 ggctaaatct gtaccggcca ctgcatgaac cacacccgcc acgctccccg catcatcagc
    10801 aggcgtctgt agtattcgca cagtgaggca tggctccgcc tccagtcggg caagctgctc
    10861 cggggtgacc tcaacctgct gacgtccacg cgtgaacagg aaccccgcac gacgaaacgc
    10921 cgaacgactg caacgcactt ctgccagcac cgtgacagaa ttgtcaccac tgacctgaag
    10981 acctgaatca tctgttgctg ccacagtgtg atgttcattc atatttgccc tccgcagggg
    11041 ccgggccgta aacccggctc accgtttaca gataatccgc gacaaccagc tccagcttgc
    11101 ccttcatctc gttagagacg gttgcgtttc cgtctgcgaa cagttcacgc tccagcagct
    11161 gcaccgcctg tttttccagc gaggtgggga caacaatatg ggtgggtttg atgccgagtt
    11221 tgcggccacc gtcagcggta aattcgcgca tggctgacca gccgtgccac agtgcatcca
    11281 gcgtcagcgg tgactgcatc atgtaggcca tctgccagaa gccgtagccc acattgcgac
    11341 gggcggaagc accgaacaca aattcgttat cggtgaatgc gcgaccttca tcgactttgg
    11401 tctgggcaac cagttcagcc ttacggcgat cctgataaat cagcggtttg attgcgcgtg
    11461 aacagtcgag cagataccag gcaggaccgc tgtaatccgc ctgtgcaccg acagagccgg
    11521 tttttgccac aaacatattg ctgaccatct gcgcatcccc ggagccatcc actttggggt
    11581 aaacaggatg ttcggtatca aagaagttct ggccgtcata gcaggccgca ttaatcccgt
    11641 cacgaagagc agcaaagacc agttcatccg gttgtgccgc agcagcgcgc cccatctcct
    11701 ggaacaacgg ggaataaata ccgaggttat cgtcttcgaa gtcatcgcgg ttaatggcga
    11761 cggtgccttc aaaggttttg ttcacgatgg catagccata ggccttcatc ttctcgatga
    11821 cgcgggagcc gatccactca cggaactgcg ggaactgccc cagccagccg taggtgttgg
    11881 atttcgaggt ggagggcacg gtcatcgcaa tttttttgta ctgcgacggg gccatggaca
    11941 tcccggcctg aaaatcagaa cggtagcccg tcatcagggc ggtgatcatc gccggtgtaa
    12001 tcggggtagg cattattgca tttcctcttt cattttcagg aactcggctt cggttttacc
    12061 cagcagtttg gcggccgcga tatcttcagc cgacagcaca gcggtggcag tctttttatc
    12121 cggcacggtc acggtgtcgg tctgaaggcc ggtcagggcc gcaaccggct ggcgggcgtc
    12181 aagctgtgca gaaagtgccg ccacgccaat ctgcccgccc agttgttcca gatagccgcg
    12241 ttcacttttg aagatgcgcc cttcggattc agccttgtcc agcacctgct ccagcgtggt
    12301 gctgccgtgc tgcgccgaca gcgcaacgta ttcggtacgc agggcgttat acgtttcaac
    12361 gggcacgtat ttcgtcagat caatactgcc gccagccggt gtgccttttg cggtttccag
    12421 ctctgccgac agactggcga cctgtgtttt caggttgtcg tgtgcatccg cacgggtttt
    12481 gatcccggtg agtgcagaca gcgccgccgt gcccagctcc ggcgtaaatt cgtcaccgtc
    12541 agccacggtc agaccgagcg ccgtcagcaa ctggcgtaat tgctcattca tggaggtatc
    12601 ctttaaagga gggtttaagg cgttataaag gtcatccgcc gacagcgcgg cgacaggatt
    12661 catgccggtc agaccggggt caccggtaat agccagcatc cgaagttcga caggttcgcc
    12721 ggtgttctcg tcatagccaa tcaccggtga aaaccagggg aattcgttat tacgcagatg
    12781 ttcaagggcc ggagggttcc acttcgggcg cacctcgaag ccacgctctt cactgaagcg
    12841 gaagttttcc ggtgaagccg tcacaaaacc ggcagccggt gcctgatgcc ccttaatcag
    12901 ggtctggtgg ttgtagtcaa tttttaccgg ctgattgagc gccacaaccc gggataccat
    12961 gcgctcaacg gtcgcacggt taatcagcca gccttcagcc ggtttttccg ggcgaccgtc
    13021 acgggccttt acccgaccgg ctggcatgat ctgacaccag tcaccgtccc cgtctgcgga
    13081 caggctgatg gcattcagaa tggcataagc aagtttgggc gtgtttttcg tcttcattcc
    13141 ggcagcataa gccgggaatt ttcagggcgg gttttgcggg atttcaggaa gtgcttccag
    13201 gggaaaaata caaaggcgct tcacgcagat tttaaagccc gttaaataca ggttagaaaa
    13261 tcacgacacg cgcctgaagg gggtaaggta atgcatttgc ataccttaaa caattaaggc
    13321 gtttctgtgg cctttttaaa gcgttttctg atttcggcca tgatctcctg ctctgccacc
    13381 tgatcaaagc ccatatacgg acgtgcacca atggccgccg gtccaggcgg catacctggc
    13441 aggccacccc actgatgaat ggccgcataa ggctcatttg atccaatcag cgcccaggta
    13501 tcgccataat ccgtggtcag gcgtcgggcc agatcgccgt tcagcgtcag gattttgccg
    13561 ggcatgtatc ccttacgggt acgccacttg cggtaaggat ctgaccagtc atgccagcgt
    13621 tcgccgtccg gctctttttc ctgttcaaac gccatttctg aggatgacag aaggctggac
    13681 gccacactgc gggccaggtc ttttccgccc cccacaaact gaagccgggc aaagacccgc
    13741 tggagacgcg taacgtcaac aacgacagcg gcatcaatgg atgacatatt gcctccgtga
    13801 aactacctgg ttaaaataaa agcaggtcgg tgtactctta actggtaaag cggcacctga
    13861 gggatatctt cacccgaagg atgtttatgt gggttcgacc cccaccaccg acttacaatt
    13921 tcccttcgag gatctccatc ttacctccct gaacagcatc aatcagagta ttcctgctca
    13981 ctctgtaggc attaatcacc acatccagct tatccggctg acgtttcaca ctataaggcg
    14041 cattgacggc aatctttgcc gttccgtcac gggtttcagt gatgtaaagc agattctgat
    14101 ggacattatc ccacagcacg gcatcaggcg cggccagcat tgccggtaat cgcccgaaat
    14161 cctccggcgt cagggcgata ccgtcattct gatgtttcac gctgtccgca tggagcacat
    14221 ttttaccgct catcgccagc aggcgggccg gtggcgttcc cgtccggctt tccaccgcct
    14281 gcgcaatact ttcggtcata aagcccagcg tgcggatatc gttaccgcct cgccgggttt
    14341 tcatgatgtt ttttgcccag atgcggaacg ccagctgccg ctccgggctg ttgttcatct
    14401 cctgaaccac catttcccgt aacgccgggc ttttcacctc gatcagttta cggatcagcg
    14461 cctggtctgt gccgaacgcc gccgaaccgg ggttatatga ccagcccaca tccggtgtca
    14521 tggttctggt gccatctgaa tatgtggtca ccggcatttc tctgacttcc ccggtctgct
    14581 tatccacgcc agcctcaaca ttgcgggtgg agagatgatc ctgaccggat gaaacagaga
    14641 gtcccattgc atccagacgg gcctgagaca atggccggac acggcagcga cagttccagc
    14701 cattgggcgg gtagtgtgta ttccagaacg gatcgtcata gcggaatacc agcccgttaa
    14761 gggcggaatg cgccgggcgg gtgcggctgt ccatcaccgc cacatactgc cagaacggat
    14821 gcgtgtccgt gttgttcatc atctgcgtgt aacgcccggc attgtaagcc acgcgggtgt
    14881 tcacgttgta aatcagcgcc agacgacggg gactgcccag ttgcacttct tccgcgttcc
    14941 ctgcgctgtc caccacaatc tgctttcccc accatcccag cttttgcagg cggggtgtca
    15001 gcgtgcggat aaatgctttc tgtgaaatcc cctcatcaac agcacgctgc acttccacct
    15061 gtagtgtggt cagtacgtcc aggcgtgcca cttttgccgc cgtgaatgaa cgggcatgaa
    15121 catccgcgtc tgtttcgaac cagttccagc tgatatgcac ccctttggcg cggaagtatg
    15181 ccaccgcctc ttttgaggga agggtggcgg cataacctaa atcaatcccc tgtgccatcc
    15241 agcatcccct tcatttccgc agcaaacatg gcatcactga gcagcgtcat caggcgggaa
    15301 tcatccattt cacggtaaag ggcgggcagg tctgccagtg cctccgccag cccccgcgtt
    15361 ctgatggcat caatgaccgg ctccagtacc gggtcgatgg cctcctgtaa ccggcgggca
    15421 ggcaccgcat cccccatgtc gtccagttca tcacgggggc tgcttttcgc ttccggcagt
    15481 ctggcagcca gcgctgtctg ctctgtcttc tcctgttttt ccggtggcat ctctttgtct
    15541 gtctgagcag gttcattgcc actctgacgg acacggaaga tggcttcacc gggcgtgggc
    15601 tgcggaatac cggtctgctc ccttatccag ggatcagaaa tatccatccc cgtactgagc
    15661 tgcatcaccg cactggtgat tttggtgata tcccccggct cttttgtctg gaaacagata
    15721 cgcggcaggc gacggatatc aatggcgtgg gcggtgttca gggcatacag cggatacacc
    15781 agatcgcggt tcagcgtggc ggccagctgg cgtaaatcag aatccctgat ttcccggcgc
    15841 acctcgttat gcacttcacc cagcgagcgc gcgcctttgt ctccggcttc cgtggtcagc
    15901 gtgccgccga gtattgcttt ggagatggaa cgctcccccc acgaaatcat ggtttcaaac
    15961 ggatcggcct gaccgttcgc tgccgcctga aactccagcg acatcccggc cgggatgatc
    16021 ccgcctgtac gtcgcccgat atccatcacc gcccgcatca gggcactttt ctgctccggt
    16081 gttgccccgg acgggtattt cccgaccttc atcggcaggc cgtacacctc cagaaattcc
    16141 gccagatcgc gcacggaata gtttttgaaa atgaacggcc agataagcgt tctgacaagc
    16201 cccgtcgcac caccgtagcc ggtgcgtgaa cgcgactgat gcactatcca gccaaagggc
    16261 tgaaacgcca ccccggcatg gctgccgtca cgcagtcgca gttcgctcaa atcatccggg
    16321 ttaaggcaga aatgcccgct gtcacgccag cggatggcgc ggatgatgtg cattttaccg
    16381 agcatcccgt gctcaatttc catgcaggaa tagcccttca ggatggcatc cgtggcgtca
    16441 aacagcatgg catcaaacca gtctgcggaa tgcagatatt cgtcgagcat ttccgcgtcc
    16501 tttttctcat tcgcgctggc gttcggtggc ggctcaatgc tccatggcac cccctgaatg
    16561 gcaagacgtc gcttgcccag ctccgcaaaa aggtgggtgt ccttttcttc aatgtcagcc
    16621 gccagatcgg actgggcaat cagatcgcca cgttcagccc cgcgaaggca ctgcgccgcc
    16681 cggttcgggg tgatacccga ggcgggatgc tcaacataac ggctggcaat ctgcggaata
    16741 tccagcgcgg tactttgcat ctccgggtca aaggagaaag gttttccgtc aagatcaatt
    16801 atgcgtccca ctaccagcac ccccgatcaa attcatgata tgcctcatcg tcatcacgat
    16861 aaccgccctc cattgcccgc gaccgctccg gcagcgcctg acaggctgat tcatccagga
    16921 tgaaaccctc catgtatgac gcccggtttg ccatacagag tgccacggca aaatcaccat
    16981 ggcggcgggc gttcgcagcc gttgcgttct ggtccttcgt gcgccccttg tcgatttgcg
    17041 ggataccatt caccaccttc acatggcgca aatcatcaag cgtggtctga tggcgggcga
    17101 cgaggatatt ctgatcttca aactcggctt tcagttttgg catccactcg ccgtaccact
    17161 tcggcgacaa catcacgcag tcgatgatgt ccggaccaaa ggccagcagc gcggcttctg
    17221 ccagatagcc gccgttaccg gtggcgtcaa acgctgcacc aaccagggcc ggaacgcggg
    17281 tcaggatgta catcatcacc tgctcctgct gggcatacgg caagttgcgc agctccacgc
    17341 gaaacacctc acgttttgcc agggattcgg tgatttccag cagcacaaag caggagaggt
    17401 caccggtgcg ggcaaagtct tccccgaagc tgaagcggga acggggattt aacgcctcta
    17461 aaagcggttt taaatgttct tcacaccagg ttaaaacttc cgattcacgc agccatgcgg
    17521 cacggctgat gaagtcgtcc ggggcttcaa acgtcagaat cgggatgtcg cggatcatcg
    17581 ccatttcaat aagtgcgtga ggaatatagg caccgccgga ttttttcggg atacagccgt
    17641 attcctcgtc ggcatcctca cgggtcgggg cgtttttgta gagatcatca cgccacttct
    17701 gctcgctttc cggtgaccat tcgcggccgg tgacataaca gatacgacgg tacagcccgt
    17761 ccgcaatggc atcatccagg gttatgcggt ggacgctgta atccttgcgt ccctcgcggg
    17821 cttcctgaat gtactgatta aacagattat cgacgccgtt atgcgtggag ataatacgca
    17881 cgcgcgcgcc ccacatggta agcgccattg ccgccttgag aagctcatcc agtgactcgt
    17941 ggaacgcggc ttcatcaatc accacatctc cctgaaggcc gcgcaggttt gacggacggg
    18001 aagacagtgc ctgaattttg aatccactgt tcggaaagcg gatcatgtag gtcagaattt
    18061 cttctttttt atcccgatcc cagaaggtct gctcatacac atcagcatct gccagctggt
    18121 taaaggcacg ggagaacagg gcgcaggcag aaatatattc cagcgccatc tcctgctttg
    18181 accccacata aaacacattg cggccaccgc gccgcttcgg ttttgcagcg gtaatgacgt
    18241 tacgcccggc ttccgcccag gtaagcccgg tacggcggga tttttccgcg atgcagacct
    18301 cgctttcatc ctcaaaccag cgggcctgat aatccagaaa caccggcata tcaccgggca
    18361 aatcaaggct gtccggcaca tccacgccca gcagggcttt ttcaccggcc agatcaattt
    18421 tgcgggtgga gacggtattc gtcagtaagg gggagagttc ttcggcgtta ttcatcagac
    18481 tttacccaac agaatcccct taatacggga ctcaagctgt tcactcatcc cgtccatgcc
    18541 gcgcagttcg tcggtgaccg cgttcgccat ctcttcagca aatgcggccc ggatttcttt
    18601 ttcacgctta atgctgcgct cggcggcact ttccgcacgc tgtgccgaca ggaggatgtc
    18661 cttgatgagg cgcatatcca cgtcgctgtc gctgttcagg gattcggtgg cggcacgcag
    18721 acggcggtac atcagggcac gggacatctc cagaatcagc gccgtagttt caccggtcgg
    18781 cttatccccc agttcagcca tcatggcttt tgtctgctcg cgcaaatcac gcagattgcg
    18841 ggcaatcagc tcattacggg aggcttcccg gctgatggcc gccggtgaaa gctgctgctc
    18901 ttccggcagg cctgcctcgc ggatcagacg gttgatttcc tcgcggatct ggacctgcgt
    18961 cagacgtttt tcacgcagca tttccagcag cggcttacgg atgctgtcgg ggagcaaatc
    19021 caccttgcgc acgcggccac gcgtcggctt gtccatcgtt acccccttgc gcgtggtttc
    19081 ttcacacccg gaacactggc gcggccttcc gccacatcct gcccccggcc ggtcagctct
    19141 gcgatgaaat aaccgttaat cagaacgcgt ttgcgcacca gtccctgttc ggcaagccag
    19201 gcaatgtggg tgtgaacggt gtcgcgggac acgcggtgac cgtaatcatc cagacagtcc
    19261 tggagcacgg attcacccag ttcaccgttg taatctgcca gcgaccgcag aatgaccaga
    19321 cgctgatctt cagtaatgaa atcactcatt gcgttttttt ccttacagcc tgctccagca
    19381 gcagttcgtt ctgataggag acagagcgga gcgtggcgtt cgtggttttc agttcacccc
    19441 gcagggtcac gatttccaca ttcagacggt tgacctcggc ttctgtcggc agtgacgaca
    19501 ggcgggtttc caccttctca acgcggtcgg agagtttttc aaaggcctcg cgggggacaa
    19561 acgttttgcg catcagcgcc atgaatatcc cgccagcggt tgccgtcgcc gaaaggatcg
    19621 gcaccacata atctttaacg atgctgaccc acatgacggg cctccatgat gttctggcag
    19681 ttcacacaac ggatggcatc cggcacagca acaagacgtg cggcggggat ctctccgcca
    19741 cagtctgcgc agacacgtct gcccggagca tccattgccc ggcacttacg tgtcagccgg
    19801 ttacaaaggg cgcgttctga tccccgctcc atcacagcct gagcacgatc tgaatcatcc
    19861 atagcttttc cttacttcgt ctttcctgcc ggaacagcag gcgttccggc catatcagta
    19921 aattcatgtt gtcgccgcgc ctccagacga cgaatactgg ccttgtccac gttgcagttc
    19981 ttgatcaccg ccagcagttg caggttgtaa gtgaccgatg ccccaaacgt gaacggctcc
    20041 ggcgcaggag gcaccaggca gtcagccagc cattcagcgg ggatcggcac cggcttcaca
    20101 ggcacgtatt tcacagacgg cccggcgcac ccgatcagcc acatcatcag gaacaggggc
    20161 agaggccgca ggcactgctg ccagcgaccg gcggacaacg ctctgctgcc cctcgctgtg
    20221 ctgaatgttc tgttgttttt cattcgtggc cgcctcactg atttgtgaaa tgagttgcag
    20281 ggtgcgttgc tggtttgcca gcaccagacg ggcttcgtcc cgttcgcgca caagcacatg
    20341 attattgtcc tcaaggcgct gcgtcttctg gtgcattccc cataccacgg cagccagcac
    20401 gctcatcacc cccagcgccg ccatacagcc tccggcaatc agggccggta agggattatt
    20461 caccgcagcc acagcacacc ccgcagcagc acaacggcac acagcagcgc aatcatgagc
    20521 ggtcgccaga agcggttcag actttcggca aatcgtagcg acataaccat tcatccgccg
    20581 aacggcgggc ctcaagaccg gggagtttca cccccttcga ataaatccag cggatgtact
    20641 gcttacagga cgcgggcatc tgcccggcat tgatcaggcg cagaagcgtg gagttgcgga
    20701 agttcgtttc accagcccag aagatccacg aagccagcgc caccgtctga ccacgggtaa
    20761 gcggcacctg aacacgccga tcaatggccg caaaggccca tttcatgtcc ttttccagta
    20821 gttccaggca ttcttcatcc gtcagggtca tacccggttt cacatccggg ccggtatggc
    20881 cgtaacagat ggttggtgta ccggtgggat cgatatacgt ggagttttct ttcccttccc
    20941 agtaaccggt gtaatgcgtg gcaatcgtga acgtcccggc accggccaga acaagggcaa
    21001 tcagcttttt acgcagtggt gcggggagtt ttggcattgt tacgctgtct catgatgttc
    21061 tctgagtcag cgtaacggtg aaggaaaggc ggcaggcttt gcggaatatc agaaaagtgg
    21121 cggttgcgtt cttgccagat gcagacggcg ctgttcgcgt atgacggtat aaatctgggt
    21181 ttctgacatc ctgtaatggc ggcgcagggt ttcaattttt tcgccctggc tccagcggga
    21241 aaaaatctca ttattgcgca gttcggtgaa aagggattca ccgaccggga gataataacc
    21301 acgcccgccc atgtacccgg cctgtgcggc cgcaacttta cgggcaagca ttcctgcctg
    21361 agcaggttca atcccctgac ggtgcagttc agcactgatc acatcaacca ggtcccgcag
    21421 cgtgccgggc cagttctttt tcagctcatc atcaggaatg tcatccagac ggtcaatcag
    21481 cgcgtgcagc tgttcgctgt caccaaacat gctcatctgt gtttcagcca tacccgcctc
    21541 cggtcatcat tacacagggc aagtttaaaa taaaaatccc gcgctgtggc gggatttaga
    21601 ggcgctgtaa atcataaccc gaaatgagct gtgtatttat tgatcatttt ccggtgtaac
    21661 tatatgataa agatcgtttt tatttttaaa aatacaaacc ctgaaggagt ttttcttatc
    21721 aaaaagcttg tgcatattgc gggttattgt tgtttcatca acatgaaaac caattatccc
    21781 aactcttaaa gcacaccagg cttgctccag tgcaaaaaca aataatttca gtctgcctga
    21841 cagcgttcca ccggaataaa ggaagatatc catgaaacaa cctccatctt tgcttttatc
    21901 gtgcccacca gaactggccc atgaactcaa tgatctggcg aaagagtata aacttacaaa
    21961 tcataatata gaagcagcaa cacttagtgc tgccggaagc gatattgtat tgatcgttct
    22021 ggccgcagct tcatgcgatg ggttctggca ggcattagcc agtatgtttc agacgtggtt
    22081 acatcgtaaa ccaacaaaac aaacaccccc ggaaaagaaa gttattcgta gcgtcattgt
    22141 tcgtagctgg gaacactatc aggagacaac taaacaaact gaattttata actttgatgc
    22201 tgatgagctg gctaaattga tagcgttata tgaaaaacca gatgaaacaa ttggttgcat
    22261 aatcccgtca gaaacggaaa ttcaggaaaa ataaaatccc gcactgtggc gggatttggg
    22321 ttaacagggg aatgattttt catacagcct gcaaacgcgc tcataactca tcactgcacg
    22381 ctccgacata tccagggctg ccagcatttt gcggcggtgc cagcgtttga gacgctccag
    22441 cacatcagag gccagctcgg ggtgttgctc cagccactgc cagttagcca caccttcgcc
    22501 gccgttctgc gcggccgtct gcgattgcac ccagcggtta agggccgttt ccgcaccatc
    22561 agaaataaag ccctgccggt gcatcacttt ccagatggcg cgaattttgg cggtcaccgt
    22621 acccggcttt aaggcccggt taaccggttt ctgacgcact ttaaaaccgc gttttttgaa
    22681 aacatccagt acgcgggata actcatccgg tgacatatcc cggcaactgg ctttaccggt
    22741 ggctgccagc agtgcggatg tataggtttc atcatccaga gcaagctcgc gttttgccac
    22801 atgaatggca gatatcagtt ttcctctcat accgtattta cctttttacg gacatggaaa
    22861 gccttttctg gctcattcca ccggatatga tgcgttggcg gaataatcat tatgtgccag
    22921 ccatctctgg ttgttattcg gcagaaaaac tcaccgctgt ataacacaat ggcctgctga
    22981 ataagcgcat ccgcagccag ctcaagcaga tatcttttat caaaaaggct gataattttc
    23041 cggcataacc accgggtgat aacaaatgcg ccgaaaaacg atgtgatata taaacaccag
    23101 aacagcagag aaatattgtc cgttatttgc tcatccattg tgtatctccc gtaattccgg
    23161 taaagccgaa atatggcacc agtgcgtcac ctgtccatcc tcatcttcaa ggcaggctcc
    23221 ggtttcttca tcaacaaaaa aaccttcata cccgatatgg ccgatgcgca gctcctgtcg
    23281 gtgaccgtac tgaacaatca gtacaacagc ctccatatca ggcgggaaag catcattcag
    23341 tgaatgccag ggataaccgc gcaaatcccc gaaataaacc ccggtcaggg ttccgccaca
    23401 gaccggcatc ccggcggcac ataccagagg ttcgtctgat tcaacaaata aggtcgttat
    23461 catggcgtta actcccgtaa tacagcgtcc ccggcagtcc ggcgatggtg ataaataaaa
    23521 ttcaccgtac tctcggataa ttcaaatttc tcgcctattt cccggaaggt aagacgtcgt
    23581 ggtgattcag catcccgtaa ttcacgaata agatgaacat catcgtcagg tatacgggtg
    23641 aagggcagca attctccgta ttttttcatg cttacgccaa tatatcgcgc ccgattgtgg
    23701 acagaatccc ggtctcgccc cagatattct cctatttgct taccggtcat cgttctggca
    23761 ttttttcgga taaatttatc ttcatcatgt ttaaatcttg gacgtttgca tttgagcaac
    23821 tccgggtaag taccgcgtaa taaaacaatc cggttatgta caccccagaa acttcttttt
    23881 accctgacag caatattctt aaccggggtg gatggataaa gcgcaatcag cagcgcatct
    23941 tcttcaggtg tccacgcacg gacatgagca ggtgcctgac cttttcgccc catgggttgt
    24001 aaaatcatca tacatcaccc cctgactcgc ttttcagcag cataaaactc atcagacaga
    24061 atttcggtca ttttctgatt tgcgggtttt acgccgcact caaggtaaat cacatcgtca
    24121 acgcagaacc acttcacagg gccaaacatg attgccgaga aatcaatacc cagccagaac
    24181 aacagggcat cggttctggc atacgtgacg ggcgaatatt cacgccacag aatgttcagt
    24241 tcatcagagg caacacgcag ggttttaggg atacgtgatg tgcgcggcgt acagctccag
    24301 ccattcgacg cagt
//
LOCUS       DAZGVQ010000072        23911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000072 DAZGVQ010000000
VERSION     DAZGVQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.072"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(29..1483)
                     /gene="xylB"
                     /locus_tag="ACHLAG_002741"
     CDS             complement(29..1483)
                     /gene="xylB"
                     /locus_tag="ACHLAG_002741"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFP0655940.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="ACHLAG_002742"
     CDS             complement(1555..2877)
                     /gene="xylA"
                     /locus_tag="ACHLAG_002742"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFP0655941.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3243..4235
                     /gene="xylF"
                     /locus_tag="ACHLAG_002743"
     CDS             3243..4235
                     /gene="xylF"
                     /locus_tag="ACHLAG_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0655942.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            4313..5854
                     /gene="xylG"
                     /locus_tag="ACHLAG_002744"
     CDS             4313..5854
                     /gene="xylG"
                     /locus_tag="ACHLAG_002744"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFP0655943.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            5832..7013
                     /gene="xylH"
                     /locus_tag="ACHLAG_002745"
     CDS             5832..7013
                     /gene="xylH"
                     /locus_tag="ACHLAG_002745"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFP0655944.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            7091..8269
                     /gene="xylR"
                     /locus_tag="ACHLAG_002746"
     CDS             7091..8269
                     /gene="xylR"
                     /locus_tag="ACHLAG_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFP0655945.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(8377..9201)
                     /gene="bax"
                     /locus_tag="ACHLAG_002747"
     CDS             complement(8377..9201)
                     /gene="bax"
                     /locus_tag="ACHLAG_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFP0655946.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            9521..11551
                     /gene="malS"
                     /locus_tag="ACHLAG_002748"
     CDS             9521..11551
                     /gene="malS"
                     /locus_tag="ACHLAG_002748"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP0655947.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            11729..12982
                     /gene="avtA"
                     /locus_tag="ACHLAG_002749"
     CDS             11729..12982
                     /gene="avtA"
                     /locus_tag="ACHLAG_002749"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFP0655948.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(13133..13606)
                     /gene="ysaA"
                     /locus_tag="ACHLAG_002750"
     CDS             complement(13133..13606)
                     /gene="ysaA"
                     /locus_tag="ACHLAG_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP0655949.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQDNQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(13708..14556)
                     /gene="yiaJ"
                     /locus_tag="ACHLAG_002751"
     CDS             complement(13708..14556)
                     /gene="yiaJ"
                     /locus_tag="ACHLAG_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFP0655950.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            14757..15755
                     /gene="yiaK"
                     /locus_tag="ACHLAG_002752"
     CDS             14757..15755
                     /gene="yiaK"
                     /locus_tag="ACHLAG_002752"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFP0655951.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            15767..16234
                     /gene="yiaL"
                     /locus_tag="ACHLAG_002753"
     CDS             15767..16234
                     /gene="yiaL"
                     /locus_tag="ACHLAG_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFP0655952.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            16352..16825
                     /gene="yiaM"
                     /locus_tag="ACHLAG_002754"
     CDS             16352..16825
                     /gene="yiaM"
                     /locus_tag="ACHLAG_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFP0655953.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            16828..18105
                     /gene="yiaN"
                     /locus_tag="ACHLAG_002755"
     CDS             16828..18105
                     /gene="yiaN"
                     /locus_tag="ACHLAG_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFP0655954.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            18118..19104
                     /gene="yiaO"
                     /locus_tag="ACHLAG_002756"
     CDS             18118..19104
                     /gene="yiaO"
                     /locus_tag="ACHLAG_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFP0655955.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            19108..20604
                     /gene="lyxK"
                     /locus_tag="ACHLAG_002757"
     CDS             19108..20604
                     /gene="lyxK"
                     /locus_tag="ACHLAG_002757"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFP0655956.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYHNFSE
                     AQRDLRHPVRTLLPDMTAHQLYQKKYQRYQHLIAALQGFHARIKEHTL"
     gene            20601..21263
                     /gene="ulaD"
                     /locus_tag="ACHLAG_002758"
     CDS             20601..21263
                     /gene="ulaD"
                     /locus_tag="ACHLAG_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP0655957.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGIILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGAANPAQVAGDFHAQIDAIWGGA
                     RA"
     gene            21256..22116
                     /gene="sgbU"
                     /locus_tag="ACHLAG_002759"
     CDS             21256..22116
                     /gene="sgbU"
                     /locus_tag="ACHLAG_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFP0655958.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTG
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            22110..22805
                     /gene="araD"
                     /locus_tag="ACHLAG_002760"
     CDS             22110..22805
                     /gene="araD"
                     /locus_tag="ACHLAG_002760"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP0655959.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDDTRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTYLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGDNAYYGQ"
     gene            complement(22837..22938)
                     /locus_tag="ACHLAG_002761"
                     /pseudo
     CDS             complement(22837..22938)
                     /locus_tag="ACHLAG_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            22942..23205
                     /locus_tag="ACHLAG_002762"
                     /pseudo
     CDS             22942..23205
                     /locus_tag="ACHLAG_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23152..23892)
                     /gene="yiaT"
                     /locus_tag="ACHLAG_002763"
     CDS             complement(23152..23892)
                     /gene="yiaT"
                     /locus_tag="ACHLAG_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFP0655960.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDHAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
BASE COUNT         5880 a         6163 c         6054 g         5814 t
ORIGIN      
        1 ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt tgctgataga
       61 ggcgacggaa cgtttcacgt cgtggctgat aagcggcata acgctgcgca tctggtagat
      121 gcgactgttc taacggtagc tgcggcaaca actcaatgag cgatttctct ggattcgccg
      181 caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc gtacggtaat
      241 cgagctgctg accgctaata tccgccagca tctgacgcca gtactcacta cgcgccccgc
      301 caccaatcaa cgtaacactt tgcggtttaa tgccgcaggc atgcacgaca tccatgccat
      361 ctgccagcgc ataacccacg ccttccagca ctgctcgcgc cagttcattg gggccatgtt
      421 gatgagtcaa accaaagaaa acccccttcg cctggggatt attgtgtggt gtacgctcgc
      481 cggaaagata aggcagaaac caaactggct cggcactttc atcagcctgt tgagctgcag
      541 cgattaaagc tgggacattg cacaggccgg ttaatttcgc gacccaatcc agacacgatg
      601 ctgcactcag cattacagac attaaatgcc aacgctgcgg tagcgcatgg caaaagctgt
      661 gtacagcgct ttctggcttg cttaagaacc cttcgctgac agcaaaatag acccccgacg
      721 tccccagcga taacattgcc tgattagcat caaccattcc cacaccaact gcaccagctg
      781 cattgtcgcc accgcctgcg acaactggca ccgtcgccat accccacgct ttcgcaactt
      841 caggtaacaa agcaccagta atttcgctgc cttcatataa tgcgggcatc tggtcacgag
      901 ataagtcgca agcctgcagc atgacgtcac tccagtcacg ctttgcgaca tccagccaca
      961 tggtgccagc tgcgtcagac atatcgctgg caaactcccc cgtcatacgc agacgcaagt
     1021 aatctttcgg taataatact ttgtcgattt gacggaatat ctccggctca tgccgctgaa
     1081 cccatagcaa tttaggcgca gtaaatccgg gcatcatcag gttgccggta atcactcgtg
     1141 attgcggaac tctcgcttcc agcaaagtgc actcttgcgc acagcgcccg tcgttccaca
     1201 aaatggcagg gcgcaatacc cgttgttgag catccagtaa ggttgctcca tgcatctggc
     1261 cggcaatacc caatgcttta acgtcctgca gagaatgctg atcgcccaga gctttcattg
     1321 cgcgatcagt tgcctgccac cactgttccg ggtcttgttc cgaccagagt ggatgcgggc
     1381 gcgaaacggt cagcttttcc gtttgcgaag caaccacctc accctgctcg ttgagcaaaa
     1441 taacttttac gcccgaggtg ccaagatcta tcccgatata catatcgatc gttccttaaa
     1501 aaaatgcccg gtatcgctac cgataaccgg gccaacggac tgcacagtta gccgttattt
     1561 gtcgaacaga taatgattta ccagattttc cagttgctcc tggcgaccac tctgatgcac
     1621 cggagacaaa ttatgttcct gagcatattt ggctaaatct gccagtgaca tttggccttt
     1681 caggatttgc tggcccaatt cgctattcca gccggaataa cgctgcgcga tgcgtttatc
     1741 cagctcgcca tcttcaatca tgcgcgctgc aattttcagc gccagtgcca tcgtatccat
     1801 cgcgccgata tgaccgtaaa acagatcata tttatcagta ctttgacgac gtactttggc
     1861 atcgaagttc agaccaccgg tggtgaaacc gcctgctttg agaatttcat acatcaccag
     1921 cgcattctct tccacactgt tcgggaactg gtcggtgtcc cagcccagtt gcgcatcgcc
     1981 acggttggcg tcgacagaac cgaacaggcc aagcgcaatg gcggtggcaa tttcatgatg
     2041 gaaagagtga cctgccagcg tcgcgtggtt agcttcaatg ttcagtttaa tctctttttc
     2101 cagaccaaac tgtttcagga agccatagac cgtcgcggca tcgtaatcat attgatgttt
     2161 ggtcggttct tgcggtttcg gttcgataag caacgtgccc tggaaaccga ttttatgttt
     2221 atgctcaacc accatctgca taaagcggcc cagctgttca cgctcctgac gcaagtcggt
     2281 atttaacagc gtttcgtaac cttcacgacc gccccacagg acatagtttt caccgcccaa
     2341 tttatgggtt gcttccatcg ctgtaacaac ttgcgttgcc gcccaactga agacttcagg
     2401 atctgggttc gtcgccgcac ccgcgccata gcgagggttt gtaaagcagt tagcggttcc
     2461 ccacagcagc ttcacgccgc tctcttcttg cttgcctgcc aggacatcaa ccatttgcgc
     2521 aaaattattg atgtactctt ttaacgacgc gccctcaggg gaaacatcca catcgtggaa
     2581 gcaataaaat ggcacatgta acttgtggaa aaactcaaat gcgacatctg ctttacgctt
     2641 cgccaacgcc agtgcctcac caggctgctg ccacggacga ttaaacgccc ccacaccaaa
     2701 catgtccgcc ccgttccagc agaatgtgtg ccagtagcag gcggcaaaac gcaagtgctc
     2761 ttccatacgc ttgcccaaca ccagttcgtc gggattgtag tgacggaatg ctaacgggtt
     2821 tgaggatttt gagccttcat aacgaacgcg atcgagctgg tcaaaatagg cttgcatatt
     2881 gaactccata atcaggtaat gccgcgggtg atggatgatg tcgtaatatt gggcactccc
     2941 tttcagttgc tcaattatgt tatttcacac tgctattgag ataattcaca agtgtgcgct
     3001 cgctcgcaaa ataaaatgga atgatgaaac tgggtaattc ctcgaagaga aaaatgcaat
     3061 aagtacaatt gcgcaacaaa agtaagatct cggtcataaa tcaagaaata aaccaaaaat
     3121 cgtaatcgaa agataaaaat ctgtaattgt tttcccctgt ttagttgcta aaaattggtt
     3181 acgtttatcg cggtgattgt tacttattaa agctgtcctc taactacaga aggccctaca
     3241 ccatgaaaat aaagaacatt ctactcaccc tttgcacctc acttctgctt accaacgttg
     3301 cggcacacgc caaagaagtc aaaataggta tggcgattga tgatctccgt cttgaacgct
     3361 ggcaaaaaga tcgagatatt tttgtgaaaa aggcagaatc tctcggcgcg aaagtatttg
     3421 tacagtctgc aaatggcaat gaagaaacac aaatgtcgca gattgaaaac atgataaacc
     3481 ggggtgtcga tgttcttgtc attattccgt ataacggtca ggtattaagt aacgttgtaa
     3541 aagaagccaa acaagaaggt attaaagtat tagcttacga tcgtatgatt aacgatgcgg
     3601 atatcgattt ctatatttct ttcgataacg aaaaagtcgg tgaactgcag gcaaaagccc
     3661 tggtcgatat tgttccgcaa ggtaattact tcctgatggg cggctcgccg gtagataaca
     3721 acgccaagct gttccgcgcc ggacaaatga aagtgttaaa accttacgtt gattccggaa
     3781 aaattaaagt cgttggtgac caatgggttg atggctggtt accggaaaac gcattgaaaa
     3841 ttatggaaaa cgcgctaacc gccaataata acaaaattga tgctgtagtt gcctcaaacg
     3901 atgccaccgc aggtggggca attcaggcat taagcgcgca aggtttatca gggaaagtag
     3961 caatttccgg ccaggatgcg gatctcgcag gtattaaacg tattgctgcc ggtacgcaaa
     4021 ctatgacggt gtataaacct attacgttgt tggcaaatac tgccgcagaa attgccgttg
     4081 agttgggcaa tggtcaggaa ccaaaagcag ataccacact gaataatggc ctgaaagatg
     4141 tgccctcccg cctcctgaca ccgatcgatg tgaataaaaa caacatcaaa gacacggtag
     4201 ttaaagacgg attccacaaa gagagcgagc tgtaagcgtt acgccccggc gcggagcggg
     4261 ggcgtgattt ctctccatgc cgcgtgaatg aattggctta ggtggagtcg ttatgcctta
     4321 tctacttgaa atgaagaata ttaccaaaac cttcggcagt gtgaaggcga ttgataacgt
     4381 ctgcttgcgg ttgaatgctg gcgaaatcgt ctcactttgt ggggaaaatg ggtctggtaa
     4441 atcaacactg atgaaagtgc tgtgtggtat ttatccccat ggctcctacg aaggcgaaat
     4501 tatttttgcg ggagaagaga ttcaggcgag tcacatccgc gataccgaac gcaaaggtat
     4561 cgccatcatt caccaggaat tggccctggt gaaagaattg accgtgctgg aaaatatctt
     4621 cctgggtaac gaaataaccc acaatggcat tatggattat gacctgatga cgctacgctg
     4681 tcagaagctg ctcgcacagg tcagtttatc catttcacct gatacccgcg ttggcgattt
     4741 agggcttggg caacaacaac tggttgaaat tgccaaggca cttaataaac aggtgcgctt
     4801 gttaattctc gatgaaccga cagcctcatt aactgagcag gaaacgtcgg ttttactgga
     4861 tattattcgc gatctacaac agcacggtat cgcctgtatt tatatttcgc acaaactcaa
     4921 cgaagtcaaa gcgatttccg atacgatttg cgttattcgc gacggacagc acattggtac
     4981 gcgtgatgct gccggaatga gtgaagacga tattatcacc atgatggtcg ggcgagagtt
     5041 aaccgcgctt taccctaatg aaccacatac caccggagat gaaatattac gtattgaaca
     5101 tctgacggca tggcatccgg tcaatcgtca tattaaacga gttaatgatg tctcgttttc
     5161 cctgaaacgt ggcgaaatac tgggtattgc cggactcgtt ggtgccggac gtaccgagac
     5221 cattcagtgc ctgtttggcg tgtggcccgg acaatgggaa ggaaaaattt atattgatgg
     5281 caaacaggta gatattcgta actgtcagca agccatcgcc caggggattg cgatggtacc
     5341 cgaagacaga aagcgcgacg gcatcgttcc ggtaatggcg gttggtaaaa atattaccct
     5401 cgccgcactc aataaattta ccggtggcat tagccagctt gatgacgcgg cagagcaaaa
     5461 atgtattctg gaatcaattc agcaactcaa agttaaaacg tcgtcccccg accttgctat
     5521 tggacgtttg agcggcggca atcagcaaaa agcgatcctc gctcgctgtc tgttacttaa
     5581 cccgcgcatc ctcattcttg atgaacctac caggggtatc gatattggcg cgaaatacga
     5641 gatctacaaa ttaattaacc aactcgtcca gcagggtatt gccgttattg tcatctcttc
     5701 cgaattacct gaagtgctcg gccttagcga tcgggtactg gtgatgcatg aagggaaact
     5761 aaaagccaac ctgataaatc ataacctgac tcaggagcag gtgatggaag ccgcattgag
     5821 gagcgaacat catgtcgaaa agcaatccgt ctgaagtgaa attggccgta ccgacatccg
     5881 gtggcttttc cgggctgaaa tcactgaatt tgcaggtctt cgtgatgatt gcagctatca
     5941 tcgcaatcat gctgttcttt acctggacca ccgatggtgc ctacttaagc gcccgtaacg
     6001 tctccaacct gttacgccag accgcgatta ccggcatcct cgcggtagga atggtgttcg
     6061 tcataatttc tgctgaaatc gacctttccg tcggctcaat gatggggcta ttaggtggcg
     6121 tcgcggcgat ttgtgacgtc tggttaggct ggcctttgcc acttaccatc attgtgacgt
     6181 tggttctggg actgcttctc ggtgcctgga acggatggtg ggtcgcgtac cgcaaagtcc
     6241 cttcatttat tgtcaccctc gcgggcatgt tggcatttcg cggcatactc attggcatca
     6301 ccaacggcac gactgtttcc cccaccagcg ccgcgatgtc acaaattggg caaagctatc
     6361 tacctgccag cactggcttc atcattggcg cgcttggctt aatggctttt gttggttggc
     6421 aatggcgcgg aagaatgcgc cgtcaggctt tgggtttgca gtctccggcc tctaccgcag
     6481 tagtcggtcg ccaggcttta accgctatca tcgtattagg cgcaatctgg ctgttgaatg
     6541 attaccgtgg cgttcccact cctgttctgc tgctgacgtt gctgttactc ggcggaatgt
     6601 ttatggcaac gcggacggca tttggacgac gcatttatgc catcggcggc aatctggaag
     6661 cagcacgtct ctccgggatt aacgttgaac gcaccaaact tgccgtgttc gcgattaacg
     6721 gattaatggt agccatcgcc ggattaatcc ttagttctcg acttggcgct ggttcacctt
     6781 ctgcgggaaa tatcgccgaa ctggacgcaa ttgcagcatg cgtgattggc ggcaccagcc
     6841 tggctggcgg tgtgggaagc gttgccggag cagtaatggg ggcatttatc atggcttcac
     6901 tggataacgg catgagtatg atggatgtac cgaccttctg gcagtatatc gttaaaggtg
     6961 cgattctgtt gctggcagta tggatggact ccgcaaccaa acgccgttct tgattttgat
     7021 aaaaattttc tcaaagccgg ttacgtatta ccggttttga gtttttgcat gattcagcag
     7081 gaaaagaacc atgtttacta aacgtcaccg catcacatta ctgttcaatg ccaataaagc
     7141 ctatgaccgg caggtagtag aaggcgtagg ggaatattta caggcatcac aatcagaatg
     7201 ggatattttc attgaagaag atttccgcgc ccgcattgat aaaatcaagg actggttagg
     7261 agatggcgtc attgccgact tcgacgacaa acagatcgag caagcgctgg ctgatgtcga
     7321 cgtccccatt gttggggttg gcggctcgta tcaccttgca gaaagttacc cacccgttca
     7381 ttacattgcc accgataact atgcgctggt tgaaagcgca tttttgcatt taaaagagaa
     7441 aggcgttaac cgctttgctt tttatggtct tccggaatca agcggcaaac gttgggccac
     7501 tgaacgcgaa tatgcatttc gtcagcttgt cgccgaagaa aagtatcgcg gagtggttta
     7561 tcaggggtta gaaaccgcgc cagagaactg gcaacatgcg caaaatcggc tggcagactg
     7621 gctacaaacg ctgccaccgc aaaccgggat tattgccgtt actgacgccc gggcgcggca
     7681 tattctgcaa gtatgtgaac atctacatat tcccgtaccg gaaaaattat gcgtgattgg
     7741 catcgataac gaagaactga cccgctatct gtcgcgtgtc gccctttctt cggtcgctca
     7801 gggcgcgcgg caaatgggct atcaggcggc aaaactgttg catcgattat tagataaaga
     7861 agaaatgccg ctacagcgaa ttttggtccc accagttcgc gtcattgaac ggcgctcaac
     7921 agattatcgc tcgctgaccg atcccgccgt tattcaggcc atgcattaca ttcgtaatca
     7981 cgcctgtaaa gggattaaag tggatcaggt actcgatgcg gtcgggatct cgcgctccaa
     8041 tcttgagaag cgttttaaag aagaggtggg tgaaaccatc catgccatga ttcatgccga
     8101 gaagctggag aaagcgcgca gtctgctgat ttcaaccacc ttgtcgatca atgagatatc
     8161 gcaaatgtgc ggttatccat cgctgcaata tttctactct gtttttaaaa aagcatatga
     8221 cacgacgcca aaagagtatc gcgatgtaaa tagcgaggtc atgttgtagc gatgatgaga
     8281 attgtcggcg tcacatcagg gaactcagca cagccacctg atgcgagcaa aagccttatc
     8341 cgaccgacaa atcagataag gctcaaggaa atgcaattac atatgcgccg cgattaaccg
     8401 ttggttatct tggtacattg cgaacaggta gttgttataa ctcttccctt tggtcgagta
     8461 gcccttcagc ttgtgaatca tcgctgtggc agtcacttcc tgatccgctt tacgcagctg
     8521 cgcacgcgat ttacggaacg aagagtaagc cgggtgcgta ttcaggttag tgacataggc
     8581 gctcaccgat tctttgacag aactaaactg tgagtaccct ttcactttac ccggcgcatt
     8641 ggtacaacgt cctttcatgc atttcatgcc gaacagattg ttattgttgc gcgccagctt
     8701 cgacgttccc caaccgcttt ctgctgcagc catcgtcgcc accatactgg tagggataat
     8761 gtctacgcgt tcaagcaagg tattccacgg gatttttcgc gtattaccag accacttcac
     8821 cttgtagcgt ttggcgatgt ctttcagacg cgcacgctca gcaggtgacc attggccctg
     8881 atactgtttt gaaatcagcc agttacgttc tgcagtaatg gctgcatttt ggctggtaat
     8941 gtaaggcatt acggtccgga gaaacgcctt tttccttggt gttccggaag ggtattttcg
     9001 caaatcagga agtgaactac tctttgcact attgcgagaa tactcttgtt tactgcttac
     9061 ctgtttatta ctagctttag ttaagtggga cttttgactc gctgttgttg tgtgcgtctt
     9121 cgctaacacc tcactcgaaa acaccagagt gagtaacata agaatcatcg ccccatatcg
     9181 tcgtatggga gtcaaaatca tcaggtctcc tggtcggatt taatcattcc aacaccttat
     9241 atttttcaca aatttgagag ttgaatctca aatcatatca aaaatagctg tcaagagcac
     9301 cccaaggaat agtccaaatc tgaaactatg tcacgtgtta acgattcaga ttggcgctaa
     9361 atcgcagaaa atgtggggtt tatcgcaaaa ttcagccgtt ttttgcgcga gatcgctcac
     9421 ccttgcttct catcctgtgg acttaccgct cagggatgag ttttgtctgg cttatcgctg
     9481 gcaaactgtc tgaaatcgca gcaataagga ctcatccacc atgaaactcg ccgcctgttt
     9541 tctgacactc cttcctggct tcgccgttgc cgccagctgg acttctccgg ggttccctgc
     9601 ctttagcgaa cagggaacgg gaacatttgt cagccacgcg cagttgccca aaggtacgcg
     9661 tccactcacg ctaaattttg accaacagtg ctggcagcct gcagatgcga taaaactcaa
     9721 tcagatgctt tccctgcaac cttgtagcaa cacgccgcct caatggcgat tgttcagaga
     9781 cggcaaatat acgctgcaaa tagacacccg ctccggtacg ccaacattga tgatttccat
     9841 ccagaacgcc gccgaaccgg tagcaaacct ggtccgtgaa tgcccgaaat gggatggatt
     9901 accgctcacg ctggatgtca gcgccacttt cccggaagga gccgccgtac gggattatta
     9961 cagccagcaa attgcgatag tgaagaacgg tcaaataacg ttacaacccg ctgctaccag
    10021 caacggttta ctcctgctgg aacgggcaga aactgacgcc cctgcccctt tcgactggca
    10081 taacgccacg gtttactttg tgctgacaga tcgtttcgaa aacggcgatc ccagtaatga
    10141 ccagagttac ggacgtcata aagacggtat ggcggaaatt ggcacttttc acggcggcga
    10201 tttacgcggc ctgaccaaca aactggatta cctccagcag ttgggcgtta atgctttatg
    10261 gataagcgcc ccatttgagc aaattcacgg ctgggtcggc ggcggtacaa aaggcgattt
    10321 cccgcattat gcctaccacg gttattacac acaggactgg acgaatcttg atgccaatat
    10381 gggcaacgaa gccgatctac ggacgctggt tgatagcgca catcagcgcg gtattcgtat
    10441 tctctttgat gtcgtgatga accacaccgg ctatgccacg ctggcggata tgcaggagta
    10501 tcagtttggc gcgttatatc tttctggtga cgaggtgaaa aaaacgctgg gtgaacgctg
    10561 gagcgactgg aaacctgccg ccgggcaaac ctggcatagc tttaacgatt acattaattt
    10621 cagcgacaaa acaggctggg ataaatggtg gggaaaaaac tggatcagaa cggatatcgg
    10681 cgattacgac aatcctggat tcgacgatct cactatgtcg ctggcctttt tgccggatat
    10741 caaaaccgaa tcaactaccg cttctggtct gccggtgttc tataaaaaca aaacggatac
    10801 tcacgctaaa gtcatcgaag gctttacacc tcgcgattac ttaacccact ggttaagtca
    10861 gtgggtccgc gactatggga ttgatggttt tcgggtcgat accgccaaac atgttgagtt
    10921 gcccgcctgg cagcaactga aaaccgaagc cagcgccgcg cttcgcgaat ggaaaaaagc
    10981 taaccccgac aaagcattag atgacaaacc tttctggatg accggtgaag cctggggcca
    11041 cggcgtgatg caaagtgact actatcgcca cggcttcgat gcgatgatca atttcgatta
    11101 tcaggagcag gcggcgaaag cagtcgattg tctggcgcag atggatacga cctggcagca
    11161 aatggcggag aaattgcagg gtttcaacgt gttgagctac ctctcgtcgc atgatacccg
    11221 tctgttccgt gaagggggcg acaaagcagc agagttatta ctattagcgc caggcgcggt
    11281 acaaatcttt tatggcgatg aatcctcgcg tccgttcggt cctacaggtt ctgatccgct
    11341 gcaaggtaca cgttcggata tgaactggca ggatgttagc ggtaaatctg ccgccaacgt
    11401 cgcgcactgg cagaaaatca gccagttccg cgcccgccat cccgcaattg gcgcgggcaa
    11461 acaaacgaca ctttcgctga agcagggcta cggctttgtt cgtgagcatg gcgacgataa
    11521 agtgctggtc atctgggctg ggcaacagtg acttttccgg cttcccgttc gtcagtacct
    11581 cgggaagccg ccaaccagga taaaatgtca gccctaatca gcgttgcagg ataaagcacc
    11641 gctcactctc caacagaccg atttgcaccc cagcaaatgt agcgttattg ttaccttcct
    11701 tgctacagag ttcgacagat atcccgctat gacattctcc ctttttggtg acaaatttac
    11761 ccgccactcc ggcattacgc tgctgatgga agatctgaac gacggtttac gcacgcctgg
    11821 cgcgattatg ctcggcggcg gtaatccggc gcagatcccg gaaatgcagg actacttcca
    11881 gacgctactg accgacatgc tggaaagtgg caaagcgact gatgcactgt gcaattacga
    11941 cggtccacag gggaaaacgg agctactcac actgcttgcc ggaatgctgc gcgagaagtt
    12001 gggttgggat atcgaagcac agaatattgc actaacaaac ggcagccaga gcgcgttttt
    12061 ctacttattt aatctgtttg ccggacgccg tgccgatggt cgggtcaaaa aagtgctgtt
    12121 cccgctggca ccggaataca ttggctacgc tgacgccgga ctggaagaag atctgtttgt
    12181 ctctgcgcgt ccgaatattg aactgctgcc ggaaggccag tttaaatacc acgtcgattt
    12241 tgagcatctg catattggcg aagaaaccgg gatgatttgc gtctcccggc cgacgaatcc
    12301 aacaggcaat gtgattactg acgaagagtt gctgaagctt gacgcgctgg cgaatcaaca
    12361 cggcattccg ctggtgattg ataacgctta tggcgtcccg ttcccgggta tcatcttcag
    12421 tgaagcgcgc ccgctatgga atccgaatat cgtgctgtgc atgagtcttt ccaagctggg
    12481 gctgcctggc tcccgctgcg gcattatcat cgccaatgaa aaaatcatca ctgccatcac
    12541 caatatgaac ggcattatca gcctggcacc tggcggtatt ggtccggcga tgatgtgtga
    12601 aatgattaag cgtaacgatc tgctgcgcct gtctgaaaca gtcatcaaac cgttttacta
    12661 ccagcgtgtt caggaaacta tcgccatcat tcgccgctat ttaccggaag atcgctgcct
    12721 aattcataaa ccagaaggag ccattttcct ctggctatgg tttaaggatt tgcccattac
    12781 gaccgagcaa ctctaccagc gcctgaaagc acgcggcgtg ctgatggtgc cggggcacaa
    12841 cttcttcccg ggactggata aaccgtggcc gcatacgcat caatgtatgc gcatgaacta
    12901 cgtaccagag ccggagaaaa ttgaggcggg ggtgaagatt ctggcggaag agatagaaag
    12961 agcctgggct gaaagtcact aaaaattgcc tgatgcgcta cgcttatcag gcctacatga
    13021 tttctgcaat atattgaatt tggaagaatt tgtaggccgg ataaggcgtt tacgccgcat
    13081 ctggcataaa caaagcgcac tattccggcg ttgagaaacg ccggaaaacg tttcaaacag
    13141 gctgctgccg tagccgatgc cgctgcactt tctcgacatc catgcactgc aacgcctttg
    13201 tcgggcaggc ttcaacacag gccggccccg tctcccgatg ccagcacaga tcgcacttaa
    13261 tcgccctcgc ctttttgcgc gaagaaacga cctccatcgc accaaacggg caagccagca
    13321 tacagctttt acagccaatg caacgtgttt gttcaacgaa aatatgccca tgttcgcggc
    13381 ttatcgcgtc aacagggcag acattcgcgc acggtgcatc ttcacactga tgacaggcta
    13441 ctgccgtggt ccagctgtgg tctttaatga cacgaatacg ggaaataaac tcgtctggtg
    13501 acaacgcagc gcaatcctga ttatcctgat gcgacactgc gcaagccact tcacaggtac
    13561 ggcaaccgat acatttcgtc gcatccgcaa taataaaccg gttcatgatc actcctttgc
    13621 actaaaacag cagagtgcca gagagcgcgt ccctactgct ttgatccaac gaaggaaatg
    13681 ttaaagtttt ggcacttgtc caaagcgtta tgtgatcgcg ccctggtcgt ccctgacagt
    13741 aaatcccagt tcattagaaa tagcctgcgc ggtttcacgc agtggtttca ggagattttt
    13801 ctcccccacc tgtttcagac gtgatgtcga aagcgaaatc gacacggcgt acggcacccg
    13861 cccatgaata ttaaacaccg gaacagcaat acaggagacg ccgagttcgt tttcttctct
    13921 gtccatcgcc gctccgcttt cacgaatgtg cgccagttcg tcgaacatcg cgggcagctc
    13981 ggtaatggta ttgcgggtta acggctggat ctcatgctga tggttttccc agtatgactt
    14041 cacgtagtcc gggtgaccaa acgccatgta gatcttgccc attgcggaac agtagagcgg
    14101 catatgctgg ccaatatagg cacgggttcg cagcatcccg gttgtgggtt ccagcttata
    14161 aatcaaaata gcgtgatcgt cttcgcggct ggagaagtta atggtttcac cagtggcgat
    14221 gttcagtgcc tcaagatgcg gagcggcgat atgaatgata ttcagcgaag acagcgcctt
    14281 ctgcccgacg gcaataaatt tggtagtcag gcggtaactc cctgcggcgg gcgcggtggt
    14341 cacatagccg caggactgta atccctgcaa taagcgatgg acggtactct tatttaaacc
    14401 agccagctcc gaaagatgcg ccaacggaca accgtttgga tagttgctca aaatctcaat
    14461 cagcatcaac ccgcgaaaca gactctggct tccggctgga cgctcttttt cctgcgccat
    14521 ctcgttctct ttttttccca tcacttcttt ccccattttg tcgcgtcctg atggtagcgc
    14581 aaagtgtgcc gtagttcacg atctcgacag ataatttata accacttgat ttttatgtat
    14641 tttgaaattc atcaatcaga ttgctttgtt aaaaagtgat cgatatattt gaaatcaagt
    14701 ttcgcatatt gaaattttaa gccaaaaaag cgatcaaaaa aacaaggaag cctgggatga
    14761 aagtgacatt tgagcagtta aaagcagcct ttaatcgggt cttaatttca cgcggcgttg
    14821 acagcgaaac ggctgacgcc tgtgcagaga tgttcgcccg caccaccgaa tccggcgttt
    14881 attctcacgg cgttaatcgt tttcctcgtt tcattcaaca actggaaaac ggcgatatca
    14941 ttcctgatgc ccaacccaaa cgtataacca gcctcggcgc aattgaacag tgggatgccc
    15001 agcgttcgat cggcaacctg acggcgaaaa agatgatgga tcgcgccatt gaactggctg
    15061 ccgatcacgg tattggtctg gtggcacttc gtaatgccaa ccactggatg cgcggcggca
    15121 gctacggctg gcaggcggcg gaaaaaggct atattggcat ttgctggacc aactccatcg
    15181 ccgtaatgcc gccgtggggc gcaaaagagt gtcgcattgg cactaacccg ctgatcgtcg
    15241 ccattccttc cacgccgatc accatggtcg atatgtcgat gtcgatgttc tcttacggca
    15301 tgttagaagt taaccgcctg gcgggccgtc agctcccggt cgatggtggc tttgatgatg
    15361 agggcaattt gaccaaagaa cctggcgtta tcgagaagaa tcgccgcatt ttgccgatgg
    15421 gctactggaa aggttctggc atgtcgattg tgctggatat gatcgctact ctcctttccg
    15481 acggcgcatc ggttgccgaa gtcacccagg acaacagcga cgaatacggc atttcacaaa
    15541 tttttattgc cattgaagtg gacaagctta tcgacggtcc cacccgcgat gccaagctgc
    15601 aacgtatcat ggattacgtt actaccgctg aacgcgctga cgaaaaccag gccatccgct
    15661 tacccggcca cgaatttact accctgctgg ccgaaaaccg ccgtaacggt attaccgttg
    15721 atgacagcgt gtgggccaaa atccaggcgt tataaggaga taagtcatga tttttggaca
    15781 tatcgcgcag cccaatccgt gccgtttgcc cgccgccatt gaaaaggcgc tcgattttct
    15841 gcgcgctacc gatttcaacg ccctggagcc gggcgttgtc gaaatcgacg gcaaaaatat
    15901 ttatgcgcaa attatcgatt taaccacacg cgaagcggtg gaaaaccgtc cggaagtgca
    15961 tcgtcggtat atcgatattc agtttctggc gtggggtgaa gagaaaatcg gcattgctat
    16021 tgatacggga aataataaag tcagcgaatc actattagag cagcgcgata ttatttttta
    16081 tcatgacagt gaacatgaat catttataga aatgataccg ggcagctacg caattttctt
    16141 tccgcaggat gttcaccgac caggttgtat tttgcaaaca gcctctgaga tacgaaaaat
    16201 tgtggttaaa gtcgcattaa cggcgctgaa ttaatcgaat aatcgtctac gccagaacga
    16261 ctgggtaatg ttattgctct aactatatta ataatttagg ttgctgcaca tgcaacttga
    16321 attatgacag acatagcctc aaggaatagc tatgaaaaaa atacttgaag caatactggc
    16381 gattaatctc gccgtacttt cctgtattgt gtttataaac attattttaa gatatggttt
    16441 tcagacaagc attttatctg tagatgaatt gtcacgttat ttatttgtct ggttaacatt
    16501 tattggcgcg attgtggctt ttatggataa cgcccacgtt caggtcacct ttctggtgga
    16561 gaaactctcc cccgcatggc aacgacgagt cgctctggtt acccattcct taatcttatt
    16621 tatttgtggc gcactggcct ggggcgcaac gctaaaaaca attcaggact ggagcgatta
    16681 ttcaccgatc ctcggtttac ccatcggcct gatgtatgcc gcttgtctgc ccaccagcct
    16741 cgttatcgca ttctttgaac tgcgtcattt atatcaactc atcacgcgta gcaattctct
    16801 aacgtcgcca ccgcaaggag cttaatcatg gctgtgctga tttttctggg ctgtctgttg
    16861 ggtggtattg ctatcggttt gcctattgcc tgggcactgt tgttgtgcgg ggcggcgtta
    16921 atgttctggc tggacatgtt tgatgtccag atcatggcac aaacgctggt gaacggagcc
    16981 gatagcttct ccctgctggc gattccgttc tttgtgctgg cgggtgaaat catgaatgcg
    17041 ggcggcttgt caaaacgcat tgttgacctg ccgatgaaac tggtggggca taaaccaggc
    17101 gggctgggct acgtcggcgt gctggcagct atgattatgg cgagcctttc tggttctgcc
    17161 gttgcggata ccgccgccgt tgccgctcta ctggtgccga tgatgcgcag cgccaactac
    17221 ccggtcaacc gggcggcggg gctgattgcc tctggcggca ttatcgcgcc aattattccc
    17281 ccttccattc cgtttattat cttcggcgtt tccagcggat tatccatcag taagctgttt
    17341 atggcgggca ttgcccctgg catgatgatg ggcgcaacgc tgatgcttac ctggtggtgg
    17401 caggccagtc gccttaattt accccgccag caaaaagcaa cgatgcagga aatctggcac
    17461 tcttttgtct ccggtatctg ggcgctgttt cttccagtca tcattattgg cgggtttcgc
    17521 tccggtctgt ttacccctac cgaagccggg gccgttgctg ctttctatgc gctgtttgtc
    17581 gccacagtta tttaccgtga aatgaccttc gccacacttt ggcatgtact cattggcgcg
    17641 gcgaaaacca cctcagtggt tatgtttctg gtggcctcgg cacaagtttc cgcctggctg
    17701 attaccattg ctgaactgcc gatgatggtt tctgatttac tgcaaccgtt ggtcgattca
    17761 ccgcgtctgc tgtttatcgt cattatggtg gctatcctga ttgtcggcat ggtcatggat
    17821 ttaacgccga cggtattaat tcttaccccg gtattaatgc ctttagttaa agaggcagga
    17881 atcgatccga tttatttcgg tgtcatgttt atcattaact gctcaatcgg cttaattaca
    17941 ccgcctatcg gtaatgtact taacgttatt tcaggggtgg caaaactcaa attcgatgat
    18001 gcagtcagag gcgttttccc ttacgtcctg gttttatatt cattattagt cgtatttgtt
    18061 tttattcccg atctcatcat cctccctttg aaatggatta attaaaagga aaatattatg
    18121 aaattacgct ctgtaaccta cgcattattc attgctggcc tggctgcatt cagcacatct
    18181 tctctggcgg cgcaatcttt acgtttcggt tatgaaacct cacaaaccga ctcgcaacat
    18241 attgcggcga aaaaattcaa tgatttattg caggagagaa ccaaaggcga gctgaaatta
    18301 aaattgtttc cggatagcac cctcggtaac gcgcaggcga tgatcagcgg cgtacgtggc
    18361 ggcaccatcg atatggaaat gtccggctcg aataactttg ccgggttatc accagtgatg
    18421 aacttgcttg atgtcccttt cctgttccgc gataccgctc acgcgcataa aacgctcgac
    18481 ggcaaagtcg gtgatgatct gaaagcctca cttgaaggta aaggactaaa agtactggcc
    18541 tactgggaaa acggctggcg cgatgtcacc aactcgcgcg caccggttaa aacccccgcc
    18601 gacctgaaag ggctgaaaat tcgcaccaac aatagcccga tgaatatcgc cgcattcaaa
    18661 gtctttggcg ctaacccgat cccgatgcca tttgccgaag tctataccgg gctggaaacg
    18721 cgcactatcg acgctcagga acacccgatc aatgtcgtct ggtcagcaaa atttttcgaa
    18781 gtgcagaagt acctttctct gacgcaccac gcctattccc cgcttctggt ggtgatcaac
    18841 aaagcgaagt ttgatggctt aaccccggag ttccagcaag cgctaatttc atctgcgcag
    18901 gaagcgggta actaccagcg caaactggtt gccgaagatc agcaaaaaat catcgacggc
    18961 atgaaagaag cgggcgtgga agtcatcacc gatctcgacc gcaaagcctt tagcgacgca
    19021 ctgggtactc aggtccgcga catgtttgta aaagatgttc cgcagggtgc tgatctactg
    19081 aaagccgtgg atgaggtgca ataaaccatg acgcaatact ggctggggtt agattgtggc
    19141 ggtagctggc tgaaagccgg gctgtatgac cgcgaaggcc gggaggcagg cgtgcagcgc
    19201 ctgccgctgt gcgcattaag cccgcagcca ggttgggcag agcgcgatat ggcagaactg
    19261 tggcaatgct gcatggctgt cattcgcgaa ctgcttaccc attccggcgt tagcggggaa
    19321 caaattgtcg gtatcggtat ctccgcacaa ggaaaaggct tgtttttgct ggataaaaac
    19381 aacaagccgc tcggtaacgc cattttgtcc tcggatcgcc gggcgatgga aatcgttcgt
    19441 cgctggcagg aagatggcat cccggaaaaa ctctacccgt tgacccgaca aaccttgtgg
    19501 accgggcatc cggtgtcgct gttacgctgg ctgaaagagc acgaaccgga acgctacgcg
    19561 caaattggct gcgtgatgat gacgcacgac tacctgcgct ggtgtttaac cggcgtcaaa
    19621 ggctgtgaag agagcaatat ttccgagtcc aacctctaca acatgagtct tggggaatat
    19681 gacccgtgcc tcaccgactg gctggggatt gctgaaatca atcacgccct gccgcctgtt
    19741 gtcggatctg ccgaaatctg cggggagatc accgctcaga cagccgtact gaccggtctg
    19801 aaagcgggta cgcccgttgt cggcggcctg tttgatgtgg tttccaccgc actctgcgcc
    19861 gggatcgaag acgaatttac ccttaatgcg gtgatgggca cctgggcggt gaccagcggc
    19921 ataacccgcg gtttacgtga cggtgaagcg catccgtatg tctatggtcg ctacgttaac
    19981 gatggtgaat ttatcgttca cgaagccagc cccacctctt ccggcaacct cgaatggttt
    20041 accgcacagt ggggagaaat ctcgtttgat gagatcaatc aggccgttgc cagcttgccg
    20101 aaggctgggg gcgatctctt tttcctgccg ttcctgtacg gcagcaacgc cggactcgag
    20161 atgaccagtg gtttctacgg gatgcaggcc attcacaccc gcgcgcacct gttgcaggcc
    20221 atctatgaag gggtggtgtt cagccatatg acccacctca accgaatgcg cgaacgtttt
    20281 actgatgttc acaccctacg cgtcactggc ggcccggcgc actccgatgt ctggatgcaa
    20341 atgctggcgg acgtcagcgg tctgcgtatc gagctgccgc aggtggaaga aaccggctgc
    20401 tttggtgcgg cccttgccgc ccgcgtcggc accggggttt atcacaactt cagcgaagcc
    20461 caacgtgact tgcgacaccc ggtgcgcacc ctgctgccag atatgaccgc ccatcagctt
    20521 taccaaaaaa aatatcaacg ttatcagcat ctcattgccg cacttcaggg ctttcacgcc
    20581 cgcattaagg agcacacatt atgagccgac cacttctgca actggccctc gaccactcat
    20641 cacttgaagc cgcgcagcgc gacgtgacgc tgttaaaaga cagcgtcgat atcgtcgaag
    20701 cgggcatcat tctctgttta aacgaagggc ttggcgcggt gaaagccttg cgcgaacagt
    20761 gcccggacaa aatcatcgtt gctgactgga aggtcgccga cgctggtgaa acgctcgcgc
    20821 aacaggcgtt tggcgcaggc gctaactgga tgaccatcat ctgcgccgcg ccgctcgcga
    20881 cggtagaaaa aggccacgca atggcgcaac gctgcggggg tgaaattcag atagagctgt
    20941 tcggtaactg gacgctggac gacgcccgcg actggcatcg tattggcgtg cggcaggcca
    21001 tttatcatcg cggtcgtgat gcacaggcca gcgggcaaca gtggggcgaa gccgatctgg
    21061 cacgcatgaa ggcgctttca gatatcggcc ttgagctttc cattactggc gggattaccc
    21121 ctgctgacct gccgctgttt aaagatatcc gcgtgaaagc gtttattgcc gggcgggcac
    21181 tggcaggcgc ggcaaatccg gcgcaagtgg ctggcgattt ccatgcgcaa atcgacgcta
    21241 tctggggagg tgcgcgtgcg taatcatccg ttagggattt atgaaaaagc gctggcgaaa
    21301 gatctctcct ggccggagcg gctggtgctg gcaaaaagct gcggttttga ttttgtcgaa
    21361 atgtcggtgg atgaaaccga cgaacggctc tcacgtcttg actggagcgc cgcacaaagg
    21421 acttcgctgg ttgccgcgat gatcgaaaca ggtgttggga tcccgtcgat gtgcctgtcc
    21481 gcacatcgtc gctttccctt tggtagccgt gacgaggcag tgcgcgaacg ggcgcgggaa
    21541 atcatgagta aagcgattcg cctggcgcgc gatctcggca ttcgcaccat ccagctggca
    21601 ggttatgacg tctattacga agaccacgac gaaggcaccc ggcaacgttt tgctgaaggg
    21661 ctggcgtggg cagtcgaaca ggcggcggca tcacaagtaa tgctggcggt ggagattatg
    21721 gatacagcgt ttatgaactc catcagcaaa tggaaaaaat gggacgagat gctcgcctcg
    21781 ccgtggttca ccgtctaccc ggacgtcggc aacctcagcg cctggggcaa tgatgttccc
    21841 gccgaactga aactgggcat tgaccgtatc gccgcgatcc acctgaaaga cacccagcca
    21901 gtcaccgggc aaagccccgg acagttccgt gatgtgccgt ttggcgaagg ctgcgtcgat
    21961 ttcgttggca tcttcaaaac gctgcatgaa ctgaactatc gcggttcttt cctgattgag
    22021 atgtggaccg aaaaagccaa agagccggtg ctggagatca ttcaggcgcg gcgctggatt
    22081 gaagcgcgta tgcaggaggc tggatttata tgttagagca actgaaagcc gacgtgctgg
    22141 cggcgaatct ggcgcttccc gctcaccatc tggtgacgtt cacctggggc aatgtcagcg
    22201 cggtagacga cacgcggcaa tggatggtaa tcaaaccttc cggcgtcgag tacgacgtga
    22261 tgaccgccga cgatatggtg gtggttgaga tagccagcgg caaggtggtg gaaggtagca
    22321 aaaagccctc gtccgatacg ccaacgtatc tggcacttta ccgtcgctat gccgaaattg
    22381 gtggcattgt gcatacccac tcgcgccacg ccaccatctg gtcacaggcc gggctggatc
    22441 tccccgcctg gggcaccacg cacgctgatt acttctatgg tgccatcccc tgcacacgcc
    22501 tgatgaccgc agaggagatc aacggcgagt atgaatacca gaccggcgaa gtgatcattg
    22561 aaactttcga agaacgcggc aggagtccgg cacaaatccc ggcggtactg gtacattctc
    22621 acggcccgtt cgcctgggga aaaaatgccg ccgatgcggt gcataacgcc gtggtgctgg
    22681 aagagtgtgc ctatatgggc ctgttctcgc gccagcttgc gccgcagctc cctgcgatgc
    22741 aaaacgaact gctggataaa cactacctgc gtaagcatgg agacaatgcc tattacgggc
    22801 agtaatccct cacgccgggg cttcatcgcc ccggcactac gaattgatat gttccttgct
    22861 gtaacgccgc ttccacgctg ctggcgttaa accggtgtgc ttgcgaaaaa tctgccggaa
    22921 atagccaacc tcattaaacc gtattgcatg gagcaggccg acaacgtcga gtcactgcat
    22981 aacctgttgc tgcccgccga tgcgccattt acgacatttg aaggcaaggg attgttcagc
    23041 cataagatcc tgattcagac gccaggttcc cggtatgaac agagcaatcc agagcaacaa
    23101 cctttgtggc attacgacgg agcaccagcc gcaccgacat ccaccggtga attaaaaacg
    23161 ccagctcacg ccggtgacaa aggtaaaact ctcattacga tcaatcatcg ggctgtcggt
    23221 aatctcttcc ggcagctcgc tgtatccggc gctcgccatc aatactacgt gctctcctat
    23281 cgggtatttt gccgtcaggc tgacataggg cacccaggca tcctgcgcgg aataacttgc
    23341 cagaccgcta cgacgggact cactctctga aatgccatag taatagtcac tgaaattctc
    23401 gtcataatag agaacgccca gcgcaggtgt cagcgacagg cgacctatct gcattttgtg
    23461 gaaaaccgat agctccccca cccagccgtt gctgttatcc agaacgtccg cagctgcaga
    23521 ggctttcacg gttccccaac gctcgtggtg ataccaggca acccccgcca tagccgtact
    23581 atcacgctta tcaagttgtt gcatggcatg atcgtcatta tcggcagggt caaattccag
    23641 cggcatccag gatgcggtca ggctaagttc atttttttca ctttgcgaca gaataaaacc
    23701 taacgtggtc tgacgaacat aaaaagaatc accttcataa ctaatcaggg gaattgcctt
    23761 tgtattttca ttataaccgc gataaggcga ttcattataa gccgcaccgg caccaatgga
    23821 taattcagaa gcagttgcgc ttgccataaa aggcaacgca aataacgcca caatattgcg
    23881 gttaattaac atgtccggta ttccatttta a
//
LOCUS       DAZGVQ010000073        23889 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000073 DAZGVQ010000000
VERSION     DAZGVQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23889
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.073"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            103..177
                     /gene="tnaC"
                     /locus_tag="ACHLAG_002764"
     CDS             103..177
                     /gene="tnaC"
                     /locus_tag="ACHLAG_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFP0655961.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            398..1813
                     /gene="tnaA"
                     /locus_tag="ACHLAG_002765"
     CDS             398..1813
                     /gene="tnaA"
                     /locus_tag="ACHLAG_002765"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFP0655962.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1904..3151
                     /gene="tnaB"
                     /locus_tag="ACHLAG_002766"
     CDS             1904..3151
                     /gene="tnaB"
                     /locus_tag="ACHLAG_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFP0655963.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLASSRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3283..4457
                     /gene="mdtL"
                     /locus_tag="ACHLAG_002767"
                     /pseudo
     CDS             3283..4457
                     /gene="mdtL"
                     /locus_tag="ACHLAG_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
     gene            4432..5391
                     /gene="yidZ"
                     /locus_tag="ACHLAG_002768"
     CDS             4432..5391
                     /gene="yidZ"
                     /locus_tag="ACHLAG_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFP0655964.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5548..6297
                     /gene="yieE"
                     /locus_tag="ACHLAG_002769"
     CDS             5548..6297
                     /gene="yieE"
                     /locus_tag="ACHLAG_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFP0655965.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWEFDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            6319..6885
                     /gene="yieF"
                     /locus_tag="ACHLAG_002770"
                     /gene_synonym="chrR"
     CDS             6319..6885
                     /gene="yieF"
                     /locus_tag="ACHLAG_002770"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFP0655966.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6939..8276)
                     /gene="adeP"
                     /locus_tag="ACHLAG_002771"
     CDS             complement(6939..8276)
                     /gene="adeP"
                     /locus_tag="ACHLAG_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFP0655967.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8443..9108
                     /gene="yieH"
                     /locus_tag="ACHLAG_002772"
     CDS             8443..9108
                     /gene="yieH"
                     /locus_tag="ACHLAG_002772"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFP0655968.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9175..9648
                     /locus_tag="ACHLAG_002773"
     CDS             9175..9648
                     /locus_tag="ACHLAG_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655969.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLRRIQ
                     VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9697..10284
                     /gene="cbrC"
                     /locus_tag="ACHLAG_002774"
     CDS             9697..10284
                     /gene="cbrC"
                     /locus_tag="ACHLAG_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFP0655970.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10346..11068)
                     /gene="yieK"
                     /locus_tag="ACHLAG_002775"
     CDS             complement(10346..11068)
                     /gene="yieK"
                     /locus_tag="ACHLAG_002775"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP0655971.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(11083..12252)
                     /gene="yieL"
                     /locus_tag="ACHLAG_002776"
     CDS             complement(11083..12252)
                     /gene="yieL"
                     /locus_tag="ACHLAG_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP0655972.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVLDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVSKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(12279..13895)
                     /gene="bglH"
                     /locus_tag="ACHLAG_002777"
     CDS             complement(12279..13895)
                     /gene="bglH"
                     /locus_tag="ACHLAG_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFP0655973.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13981..15375)
                     /gene="bglB"
                     /locus_tag="ACHLAG_002778"
     CDS             complement(13981..15375)
                     /gene="bglB"
                     /locus_tag="ACHLAG_002778"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFP0655974.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLEAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15394..17271)
                     /gene="bglF"
                     /locus_tag="ACHLAG_002779"
     CDS             complement(15394..17271)
                     /gene="bglF"
                     /locus_tag="ACHLAG_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFP0655975.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(17404..18240)
                     /gene="bglG"
                     /locus_tag="ACHLAG_002780"
     CDS             complement(17404..18240)
                     /gene="bglG"
                     /locus_tag="ACHLAG_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFP0655976.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(18526..19251)
                     /gene="phoU"
                     /locus_tag="ACHLAG_002781"
     CDS             complement(18526..19251)
                     /gene="phoU"
                     /locus_tag="ACHLAG_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFP0655977.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(19266..20039)
                     /gene="pstB"
                     /locus_tag="ACHLAG_002782"
     CDS             complement(19266..20039)
                     /gene="pstB"
                     /locus_tag="ACHLAG_002782"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP0655978.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(20130..21020)
                     /gene="pstA"
                     /locus_tag="ACHLAG_002783"
     CDS             complement(20130..21020)
                     /gene="pstA"
                     /locus_tag="ACHLAG_002783"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFP0655979.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(21020..21979)
                     /gene="pstC"
                     /locus_tag="ACHLAG_002784"
     CDS             complement(21020..21979)
                     /gene="pstC"
                     /locus_tag="ACHLAG_002784"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFP0655980.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(22066..23106)
                     /gene="pstS"
                     /locus_tag="ACHLAG_002785"
     CDS             complement(22066..23106)
                     /gene="pstS"
                     /locus_tag="ACHLAG_002785"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFP0655981.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(23354..>23889)
                     /gene="lpfD-O113"
                     /locus_tag="ACHLAG_002786"
     CDS             complement(23354..>23889)
                     /gene="lpfD-O113"
                     /locus_tag="ACHLAG_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFP0655982.1"
                     /translation="IVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEINNLQPVY
                     FDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEPNTDNSM
                     VVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPASLTGARP
                     APGQFTATATITVEIVR"
BASE COUNT         6023 a         6033 c         5661 g         6172 t
ORIGIN      
        1 ccccgaacga ttgtgattcg attcacattt aaacaatttt agaatagaca aaattcttga
       61 gtattatact gtagcctcgt gtctttcgag gataagtgca ttatgaatat cttacatata
      121 tgtgtgacct caaaatggtt caatatcgac aataagattg tcgatcaccg cccttaattt
      181 acccttctgt agccatcacc agagccaaac cgattagatt caatgtgaac gattagtttg
      241 ctatatctta attttgcctt ttgcaaaggc catctctcgt ttatttactt attttagtaa
      301 atgatggtgc ttgtatatat acatggcgaa ttaatggata ttgcagatgt aatattcaca
      361 gggatcactg taattaaaat aaatgaagga ttatgtaatg gaaaacttta aacatctccc
      421 tgaaccgttc cgcattcgtg ttattgagcc agtaaaacgt actactcgcg cttatcgtga
      481 agaagcaatt attaaatccg gtatgaaccc gttcctgctg gatagcgaag atgtgtttat
      541 cgatttactg accgacagcg gcaccggggc ggtaacccaa agtatgcagg cagcgatgat
      601 gcgcggcgac gaagcctaca gcggcagccg cagctactat gcgttagccg agtcagtgaa
      661 aaatatcttt ggttatcaat acaccattcc gactcaccag ggccgtggcg cagagcaaat
      721 ttatattccg gtactgatta aaaaacgcga gcaggaaaaa ggcctggatc gcagcaaaat
      781 ggtggcattc tctaactatt tctttgatac cacgcagggc catagccaga ttaacggctg
      841 taccgtgcgt aacgtctata tcaaagaagc cttcgatact ggcgtgcgtt acgactttaa
      901 aggtaacttt gaccttgagg gattagaacg cggtattgaa gaagttggcc cgaataacgt
      961 gccgtatatc gttgcaacca tcaccagtaa ctctgcaggt ggtcagccgg tttcactggc
     1021 aaacttaaaa gcgatgtaca gcatcgcgaa gaaatacgat attccggtgg taatggactc
     1081 cgcacgcttt gctgaaaacg cctatttcat caagcagcgt gaagcagaat acaaagactg
     1141 gaccatcgag cagatcaccc gcgaaaccta caaatatgcc gatatgctgg cgatgtccgc
     1201 caagaaagat gcgatggtac cgatgggcgg cttgctgtgc atgaaagacg acagcttctt
     1261 tgatgtgtac accgagtgca gaaccctttg cgtggtgcag gaagggttcc cgacatatgg
     1321 cggcctggaa ggcggcgcga tggagcgtct ggcggtaggt ctgtatgacg gcatgaatct
     1381 cgactggctg gcttatcgta tcgcgcaggt acagtatctg gtcgatggtc tggaagagat
     1441 tggcgttgtc tgccagcagg cgggcggtca cgcggcattc gttgatgccg gtaaactgct
     1501 gccgcatatc ccggcagacc agttcccggc acaggcgctg gcgtgcgagc tgtataaagt
     1561 cgccggtatc cgtgcggtag aaattggctc tttcctgtta ggccgcgatc cgaaaaccgg
     1621 taaacaactg ccatgcccgg ctgaactgct gcgtttaacc attccgcgcg caacatatac
     1681 tcaaacacat atggacttca ttattgaagc ctttaaacat gtgaaagaga acgcggcgaa
     1741 tattaaagga ttaaccttta cctacgaacc aaaagtattg cgtcacttca ccgcaaaact
     1801 gaaagaagtt taattaatac tacagagtgg ctataaggat gttagccact ctcttaccct
     1861 acatcctcaa taacaaaaat agccttcctc taaaggtggc atcatgactg atcaagctga
     1921 aaaaaagcac tctgcatttt ggggtgttat ggttatagca ggtacagtaa ttggtggagg
     1981 tatgtttgct ttacctgttg atcttgccgg tgcctggttt ttctggggtg cctttatcct
     2041 tatcattgcc tggttttcaa tgcttcattc cgggttattg ttattagaag caaatttaaa
     2101 ttatcctgtc ggctccagtt ttaacaccat caccaaagat ttaatcggta acacctggaa
     2161 cattatcagc ggtattaccg ttgccttcgt tctctatatc ctcacttatg cctatatctc
     2221 tgctaatggt gcgatcatta gtgaaacgat atcaatgaat ttgggctatc acgctaatcc
     2281 acgtattgtc gggatctgca cagccatttt cgttgccagc gtattgtgga taagttcgtt
     2341 agcctccagt cgcattacct cattgttcct cgggctgaag attatctcct ttgtgatcgt
     2401 gtttggttct ttcttcttcc aggtcgatta ctccatcctg cgcgatgcca ccagcaccac
     2461 tgcgggaacg tcttacttcc cgtatatctt tatggctttg ccggtgtgtc tggcgtcatt
     2521 tggtttccac ggcaatattc ccagcctgat tatttgctat ggaaaacgca aagataagtt
     2581 aatcaaaagc gtggtatttg gttcgctgct ggcgctggtg atttatctct tctggctcta
     2641 ttgcacgatg gggaatattc cgcgcgaaag ctttaaggcg ataatctcct caggcggcaa
     2701 cgttgattcg ctggtgaaat cgttcctcgg caccaaacag cacggcatta tcgagttttg
     2761 cctgctggtg ttctctaact tagctgttgc cagttcgttc tttggtgtca cgctggggtt
     2821 gttcgattat ctggcggacc tgtttaagat tgataactcc cacggcgggc gtttcaaaac
     2881 cgtgctgtta accttcctgc cacctgcgtt gttgtatctg atcttcccga acggctttat
     2941 ttacgggatc ggcggtgccg ggctgtgcgc caccatctgg gcggtcatta ttcccgcagt
     3001 gcttgcaatc aaagctcgca agaagtttcc caatcagatg ttcacggtct ggggcggcaa
     3061 tcttattccg gcgattgtca ttctctttgg tataaccgtg attttgtgct ggttcggcaa
     3121 cgtctttaac gtgttaccta aatttggcta aatccttcaa gaagccagcc attcgctggc
     3181 ttcttgcctc tcaggaaatc acttatgtcc aaatggcaac tcgcctgatt ctccttcacc
     3241 acgtatgctt tgcgtcacct tactattagg acgctttagc ccatgtcccg ctttttaatt
     3301 tgtagttttg ccctggtttt actttatccc gccgggattg atatgtacct cgttggttta
     3361 ccgcgcatcg ccgacgatct caatgccagc gaagcgcagt tgcatattgc gttctccgtt
     3421 tatctggcgg gcatggcagc tgcgatgtta tttgccggta agtggccgat cgttcaggga
     3481 gaaagcctgt cgccatcccc ggcgcggcgc tatttattat cgcttcggtg ttctgttcac
     3541 tggctgaaac cagcacgtta tttcttgcag gccgatttct acaggggttg ggcgcaggct
     3601 gttgttacgt agtggcgttc gccattttgc gcgacacgct ggatgatcga cgtcgggcta
     3661 aagtgctgtc attactcaac ggtattacct gcatcattcc ggtgttagcg ccagtgctcg
     3721 gacatctgat tatgcttaaa ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg
     3781 gcatcgcggt actgatgttg tctttgttta ttttaaaaga aacgcgccca gcgtcccccg
     3841 ctgcttcgga caaaccacga gaaaatagcg agtcgctgct taatcggttt ttcctcagcc
     3901 gtgttgttat caccaccctc agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg
     3961 tattgctgat ggaaatcatg ggttttgagc gcggagaata cgccaccatt atggcgttga
     4021 ctgctggcgt cagcatgacc gtttcattct ccacgccatt tgcgctggga atttttaagc
     4081 cacgtacgtt gatgatcacc tcgcaggtgt tattccttgc agcggggatc acccttgccg
     4141 tttcaccttc ccatgcggtt tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg
     4201 taggttttgg cgtagcgatg agtcaggcgt taggaccatt ttcattacgc gcgggcgtag
     4261 ccagctcgac cttaggtatt gcgcaggttt gcggttcgtc actgtggatt tggctggcag
     4321 cggtggttgg tatcggcgca tggaatatgc tgatcgggat tctgattgcc tgtagcatag
     4381 tgagcctgtt gctgattatg ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa
     4441 tccatcacca cgcttgatct caatctgctg ctctgtctgc aactgctgat gcaggagcgc
     4501 agcgtgacca aagcggcgaa gcggatgaac gtgacccctt cagcggtgag taagtcgctg
     4561 gcaaagttaa gagcgtggtt tgacgacccg ctctttgtga actcaccgct gggcctgtcg
     4621 cccacaccgc taatggtcag catggagcaa aatctggcgg aatggatgca aatgagcaat
     4681 ctgctgctgg ataaaccgca ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg
     4741 gaatcgccgc tgatgatgat catgcttaat gcgctgtcga aacggatcta ccaacgttac
     4801 ccgcaggcga ccatcaaact acgtaactgg gattacgatt ccttagatgc cattactcgt
     4861 ggtgaagtgg atattggttt ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta
     4921 agctcgctac cgttagccat tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg
     4981 ttacgcaaag atcatccggc actgcatgaa acgtggaatc tggacacctt tttacgttat
     5041 ccgcatatca gcatttgctg ggaacagagc gacacctggg cgctggacaa tgtgttacag
     5101 gagctggggc gcgaacgcac gattgctatg agcctgccgg aatttgagca gtcactgttt
     5161 atggcagcgc aacccgacaa tctgctactg gcgaccgcgc cgcgctactg tcagtactac
     5221 aatcaactcc atcaactgcc gttggttgct cttcctctcc cgtttgacga aagccagcaa
     5281 aaaaagctgg aagttccttt taccctgctg tggcataaac ggaacagcca taatccgaag
     5341 atcgtctggt tacgggaaac cattaaaaac ctttacgcgt cgatggcata accgaatcgt
     5401 atgaaacgcg acccaatttc acaataaaat gtaaaaaagt tgtaataagc ttgtctgaat
     5461 cgaactttta gccgctttag tctgtccatc attccagtaa atgattactc ttgtattcat
     5521 aatggaccat taagcatgga gcgaaaaatg gcgactcact ttgcccgagg gattttaacg
     5581 gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt
     5641 ccccctcatc gtcaaagccg ttttctggcg tctagaggtt tactcgcaga actgatgttc
     5701 atgctgtatg gcattggcga attgccggaa atcgtcaccc tgccgaaagg taaaccggtt
     5761 ttcagtgata aaaatttgcc ttcgttttct atttcctatg ccgggaatat ggttggcgtg
     5821 gcgttaacaa ccgaaggtga atgtggtctc gatatggaac tacagcgtgc gacgcgcggg
     5881 tttcatagcc cacacgcgcc cgataaccac accttttcca gcaatgaatc gctatggatc
     5941 agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg
     6001 ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg atctgcagct gctgcccatt
     6061 gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag cgttatgcga cgcggaggac
     6121 gtgctggtgt ggtccgtggc ggtcacgccc acgattgaaa agctcagtgt ctgggagttc
     6181 gatggcaaac acggctggaa aagcctgccg gatattcaca gccgcgccaa caatcctacc
     6241 agccggatga tgcgttttgc ccagctctct accgtgaagg ctttttcgcc aaattgatag
     6301 acaaccacag gagtcatcat gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg
     6361 cgcaaaggct catttaatgg catggttgca cgtaccctgc cgaaaattgc tccggcgagc
     6421 atggaagtca atgcgttacc ttccattgcc gacattccct tgtatgacgc tgacgtacag
     6481 caggaagaag gttttccagc aacggttgaa gctctggcgg aacagatccg tcaggctgac
     6541 ggtgtggtga tcgtcacgcc ggaatataac tactcggtac cgggtgggct gaaaaatgcc
     6601 atcgactggc tttcccgcct gccggatcaa ccgctggccg gtaaaccggt gttgattcag
     6661 accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt atcacctgcg ccagattctg
     6721 gttttcctcg atgcaatggt gatgaacaag ccggaattta tgggcggcgt gattcagaac
     6781 aaagttgatc cgcaaaccgg agaagtgatt gatcagggta cgctggacca cctgaccggg
     6841 caattgaccg catttggtga gtttattcag cgagttaaga tctaaataaa aaacccgcca
     6901 gcaatcatgc atggcgggtt tttaacgcgc tatcgatttt agtgagcgtc gataaagaca
     6961 atcttcagga taaacagcag cgcaacgatg attacgcacg ggcttagatc acgcagacgt
     7021 ccggtaccga ttttcatcac gcagtaggag ataaagccca gcgcgatacc ttcggtaatc
     7081 gagaagctga acggcatcat cacggcggta ataaacgccg gaacagattc agtaagatcc
     7141 tgccagttca cgcgtgccag actggaagtc atcagcacgc caacgtaaat cagcgcacca
     7201 gctgcagcgt agcctggcac catccccgcc agcggcgaca gaaagataac cagcaggaac
     7261 agcagaccaa caaccactgc cgtcagaccg gtacgaccgc caaccgatac gccggaagag
     7321 gactcaatat aagccgtaac ggaagaagta ccgataaacg aaccggtcac ggaagagata
     7381 ctgtcgacat acagcgcctg cttcatgcgc gggaatttac ccttctcatc cgccaggcct
     7441 gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa acaggttgac cagcatgaaa
     7501 gagaaaatca cccctgccag cccgaggtta aacgacccgg ctaaatctac atgacccaca
     7561 actgtcatta cgctcggcgg cgcagaaacg atgccattgt agtgcacatc acccagcatc
     7621 cagcccagca gcgtcgtcac cacgatagaa accagcaccg ctgcgtgaat gttgcgcgag
     7681 gccagaatag caatgatgaa gaagccgagg atccccagca gcacgctgtg agaagtcagg
     7741 ttaccgatgc tgaccagcgt ttccgggtta gcgacaatca cacctgcatt cttcagcccc
     7801 atcataccaa tgaacagacc gataccgctg gtgatgccca cacgcagact taccggaatg
     7861 ttggcaatca tccagtagcg aacgcggaaa atcgtcagta acagcagacc aatcgcaccc
     7921 cagaagattg cgcccatccc gacttgccac ggcaagccca tcgcctgtac aacgacaaaa
     7981 gcgaagaacg cattcaggcc catagcgggt gccagtgcaa ctggcaggtt agcaaacagt
     8041 cccatcataa tactgccgaa tgcagcgatc agacaggtag tgacgaagac ggcgctggta
     8101 tccatgccag caacgccaag aatttgcggg ttaacaaaaa cgatgtaaac catcgtcagg
     8161 aaggtggtaa aaccggcgat cacttcggtc cgtgccgtcg tgccatgttc gcgcagttta
     8221 aacacgcgtt ccagcatccc ctggccagaa gcctgggttg tgtgttgttg actcattatc
     8281 tatttccgaa caaggaggga aaatccgtcg ctatcgtata ccaaaatgag acaatatgga
     8341 cggctgaaga taactttttt tcattgtatt tgcttatacg gcaacgattg cgtcatgcga
     8401 attggcatga cgaaaacgtt aaactaacga taaaggaaag acatgtccca gatagaagcg
     8461 gtatttttcg actgcgacgg tacgctggtc gacagtgaag tcatttgctc ccgcgcatat
     8521 gtaacgatgt ttcaggaatt tggtattacg ctcgatcctg aagaggtatt caaacgtttc
     8581 aaaggtgtaa aactgtacga aattatcgat attgtttccc ttgaacatgg tgttacgtta
     8641 gcgaaaacag aagctgaaca cgtttaccgt gcagaagtcg ctcggctgtt cgattcagaa
     8701 ctggaagcca tcgaaggggc tggagcgctc ctgtcagcga tcactgcgcc aatgtgtgtg
     8761 gtatctaacg gcccaaataa caaaatgcag cattctatgg gcaagctgaa tatgttgcac
     8821 tacttcccgg ataaactgtt cagcggctac gatattcagc gctggaagcc agacccggcg
     8881 ttaatgttcc atgcggcaaa agcgatgaat gtaaatgtag aaaactgcat tctggttgat
     8941 gactcagttg ccggtgcgca atctggtatc gacgcaggta tggaagtgtt ctacttctgc
     9001 gccgacccgc acaataagcc gatcgttcac ccgaaagtca ccacctttac ccatctttcg
     9061 cagttaccgg aactgtggaa agcgcgtggt tgggatatta cggcatagtt cttcacactc
     9121 ccttcactta ccccgcttaa attggcgctc aaaggtaagt aaagggagtt tgatatgtct
     9181 gtttcacgtc gggtaataca tcacggactt tattttgcgg ttttaggacc gttaattggt
     9241 gttctgtttc ttgtcctcta catattcttc gcaaaagaac cgctggttct tttgcgaaga
     9301 atacaggtac ttccattgtt tctcttattg tcgattacta cgggagttat tcctgcattg
     9361 ttaaccggcg tgatggtcgc ctgtctgccg gaaaagatcg ggtcacagaa aaattatcgt
     9421 tgtctggctg gtggcatagg tggcgtcgtt atcaccgaga tctattgtgc agttattgta
     9481 catattaagg gcatggcttc ctcggagttg tttgaaaaca ttctttctgg tgacagtctc
     9541 gttgtccgca tcattcctgc attgctggca ggtgtggtga tgagcagaat cattacccgt
     9601 ctgcccggat tggatatttc atgtcctgaa acagactctt taagttaagc gggatacttt
     9661 atctttgggc tactcaaaag cagacaggat gttactatga ctcaaaatat caggccgtta
     9721 ccccaattca aatatcatcc caagccactg gaaacaggcg catttgaaca ggataaaacc
     9781 gtagagtgcg attgctgtga acaacagacg tcagtttatt actcgggtcc cttttattgc
     9841 gttgatgaag ttgaacatct ctgtccgtgg tgtattgcgg acggttctgc tgctgaaaag
     9901 tttgcaggta gttttcagga tgatgccagc atagaaggtg ttgaatttga gtatgatgaa
     9961 gaggacgaat ttgccggtat taagaacaca tatcctgatg aaatgctgaa agagttggtt
    10021 gaacgcacgc caggttatca tggatggcaa caggaattct ggcttgcgca ttgtggcgat
    10081 ttctgtgctt ttatcggcta tgtgggctgg aatgatataa aagatcgcct cgatgaattt
    10141 gccaaccttg aagaagattg tgagaatttc ggtattagaa attctgatct agctaaatgc
    10201 ctgcaaaagg gtggtgattg tcagggttat ctcttccgct gtctccactg cggcaagctg
    10261 agactgtggg gtgatttttc gtagttattt aaataatgag aacaggccgg agcgtaattc
    10321 atacatccgg ccttatttct tcagcttaac cgagcgctaa ttctgcggca gcggctttat
    10381 cagcgattac catcagcgaa ggatgcagct gcaacacgga agctggaaca tcttcggtaa
    10441 ccggaccttg cagaacattt ttcagtgcct gcgctttacc tgcgccactg acaattatca
    10501 gcaagttttt cgccgccatg atgcttttcg gccccatcgt cacgtagctg tcaggcacta
    10561 acgaaaagtc gccgcccaac tcaccatgcg ccacaatatc aaccatctct ccctggatcg
    10621 ggaactccac ggtttgctca tggaaatggg tcgtattcgg caaattgcca caaaaatgac
    10681 catctgcacc taatcccagc accaccaaat ccaggccgcc ttcacgcgcc agtttctgat
    10741 catgctcgcg gtagttatca atagtgagct tctggatatt ctcttctttg atccccgcag
    10801 gggtgaaaaa cagattacgc agattggtaa tcgttacacc ttctccctct ttgccgcgaa
    10861 agggaatttc atcgaaatta tagaaatagc agttatcgta ccagggctta cctttaacca
    10921 gggtggtgag gtattcatac atgcctttgg gcgtgctacc ggcggtaatt gccaggttaa
    10981 cacgacgcgt cttcgacata taaccaagca gatggtgtgc cgcgacacgg ctcatttcct
    11041 ggtaatcttc ggtaatgatt aatttcatct tagtgcctta ttttatttaa ataatttctg
    11101 tacgaaggct gcataggcag gccgccagac atccatttca tggttcaggc cgggatattc
    11161 ctggtaatca aacttaattt ttttctgctc aagctcagtt ttcagcccgg cgatatcctt
    11221 gccggttacg acatctttat caccaacgac cacagtaaaa ttacgtagtt gctggttaat
    11281 agctgccgga tcgttcagcc gggccgcgac accttcatcc ggtacggttg tcgtggtaac
    11341 accactgaat gtggccagcc agccaaagct ttccagatga ttcattcccg ataccagcgc
    11401 ctgataccca ccttgtgaaa gccctgccag tgcgcgacca tcggcatctt tactgacgtt
    11461 aaaacgcttg ctaatcagcg ggataatatc gttcatcagt tcgcgatctg ccgctttagc
    11521 attcagcgga taaaagactt tacgtctttc ctgaggcacg aaatcttcgg gaataatgcc
    11581 cttcgcatcg gtttcggtat ccgggatcac caccagcatc ggtttaattt tcccttcagc
    11641 cagcaggtta tccatgattt gcgggatacg cccctgatcg atagcggaac gtccggtatc
    11701 accaaaaccg tgatagaaat agagcactgg caaaggctcg cccatgccgg tgtatcctgg
    11761 cggggtccag acatacatct gacgttcaga ttgcaatgcg ttggagtggt aagttatggc
    11821 gatcaaatca ccatgcgcaa cagaacgcgt atccagataa ctgcctggca ccagaatcat
    11881 actggagtta acctggcgct gaggcttagt cattgcggtg cctgtatcaa tgctgcgtac
    11941 accatcaaca ttgaaaaaat actcgtacag gttgcctttc aggataggtg tgcgccacga
    12001 ccagactcct gcttcgtctt tggtcatcgg gtgaatattg tccagaaccg gaacacccac
    12061 cactacagag acattttttg ccccaggggc aaagtagcga aaggtcacgc tgttatcggc
    12121 attgacctga gtcacatact gtttcaccgg gatagtgggt gccgggctgg ctggcatatc
    12181 cgctgctatg gcccactgag cagaaatacc gctggcaata gccagcgtta aagcagcaat
    12241 ttttatattc atcgtaatat tcctttcatt aagttccatt accaccagat ttcagcctgg
    12301 gcaccgacag caaactggtc gtctttattg tcttcgaagg tgaagccatc cagttcgttt
    12361 tccagggctt tgatatacgt ggcgtagaaa cgaatttccg gacgcgaggt caacatactg
    12421 gtattgactt taaaggtatg gaagagcgtg gttttataac cagactcatt aaatttatta
    12481 ctattcgcat ctttgttttg ctgggtgaaa tagcccagtt caacacctgt ctggttatat
    12541 tggtcccaaa tataggccgg gcgaacaacc gcacgaatag attcgaaatc agagtgtgcg
    12601 cctgtttcgt agctataaat atcgttaccg aaggagtaaa caatcgcgtt agccacaatg
    12661 aaatgatcgc cgatataggc ttcgccctgt gaagtcagac gaactgctgt tccgccggtg
    12721 tgatcaccat aataacgacc attaaaggtg gtaaatggac ttgcgccagc ataacggcca
    12781 aagttactgg cgatagagtt attcgcaacc aggaaggaga attcgttgaa gccaccttta
    12841 tcaaatttct gcgttaaaga tgtgccaaac atccaggtat ctttccagtc ataatagccg
    12901 ttattatcct gattatcttt ttccgatgcg ctttcgtttg ccgtgacata acgaccactc
    12961 accattaagg tcgctttatc ccataacggg atatctttat agcgtaaatc aatagtattg
    13021 gtattaatct gctgtttgtt ttgcaggctg cgatcgtaat catcgacatc ttcgcgaacc
    13081 agcgcgatat caattttacc cggaccgact ttccagtttt ccagacctac acccgctgcg
    13141 gcatcagtac gctgcgtttt ccagtccagc atctggattt caattttcgg cgcaccgtgt
    13201 ttacccaccc agaaatcagc ctctggcgca aagggcagga aacctttggt ggtaacgtac
    13261 atatcggaga actgcataaa gttctcgcca ccggcgttat cgccaaacca gccggtagag
    13321 tactgctgac caacgttacc atccatcatc acaacggcat caacccgttt gccgttttcg
    13381 ttgtagacac gttgttttaa ctgcaaatca aaccagccgg agtattcgtt accaaagcgg
    13441 cccagagaac caatcgccca tgatttaggt gaaccatgag aggcggtccc ccaaccagaa
    13501 cggtaatagc cgttgtaact aaagccaatt tcatctttca caaacttact gaaatctttc
    13561 agagtcatgg aagaataaat cgagcctgtc tcactggcat ttttctcttc atttttgacc
    13621 agtacagcat caggttttgc ggcactactg gtcgggaacg gattggcggc atacccttga
    13681 tcattcgtac ttacgctacg attcaccgtc gctggcgtat attttttctt ctcttcatct
    13741 ttatactttt tgagttcgct ttgcgtttct cttaacgctt tttctaataa ctcaaggcga
    13801 tgttccaccg ttaatgattc agccaatgat tgtgcagaaa aggctaacgg tgccattaat
    13861 aagatggcag aggtaataag atttcgtcta aacataatat ccctttatgg tgcaaagaaa
    13921 gaattaacgt atcgcatcca gactgttctg aatgcgacga taattaaggt gctttattgg
    13981 ttattttttt aatgacagcc cccgcgtttt gatcacttct gcataccatc cgaagctttt
    14041 cttacgcgtt cttgtgaggc ttccttcgcc attatcatca cgatccacat aaataaagcc
    14101 gtagcgctta gacatttgtg aatgagatgc actgactaaa tcaattggcc cccaactggt
    14161 gtaccccata atatccacac catcggcaat cgcttcattt acctgtacca ggtgatcgtt
    14221 taaataggca attcgataat cgtcctgtat cgaaccatcc gcttcaacgc tgtcttttgc
    14281 gcctaatccg ttctcgacaa taaataacgg tttttgataa cgatcccaaa gcgtatttaa
    14341 cagaacccgt aatccaaccg gatcaatttg ccacccccac tctgaacttt tcagatgcgg
    14401 attggggatc atattcagta tgttgccctg cgcattttta ttaatgcttt cgtcgtggga
    14461 aacacaacca gtcatgtaat aactaaaaga gatgaaatct acggtatgtt ttaaatcttc
    14521 tgcgtcactt tcagtcatct caatggtgat attgtggtcg cggaagaaac gctgcatata
    14581 gccgggatac tgaccacgcg cctgaacatc accaaagaac atccagcgcc ggttctcttc
    14641 catggcctcc aacatatcct gtggctggca ggtgaggggg taaaccagcc cgccgagaag
    14701 catattgccg attttcgctt cggggatcag gctatgacag gctttaactg cccgcgcact
    14761 ggcaaccagt tgatgatgga tcgcctgata aacttccgcc tcgccactct cttctgccag
    14821 ccccacgccc gtgaatggcg cgtgtaacga catattgatt tcattaaacg tcagccataa
    14881 cgctacttta tgttggtagc gggtaaagac cgtgcgggcg taatgctcga agtgatcgat
    14941 gaccgcccga ttagcccaac cgccgtagtt tttcaccagc ccatatggca tttcgtaatg
    15001 ggataacgtt accagcggct tgatccccgc ctgcgccatt tcatcaaaca gccgatcgta
    15061 aaacgctaac cccgcttcat tcggttcggc ttcgtcgccc tgagggaaaa ttcgcgccca
    15121 ggcaatggaa atacgcagac aggtgaagcc catctcggca aataacgcga tatcttccgg
    15181 gtaacggtga taaaaatcga tggcgacatc tttgatattc tctttcccca ggatgcgcgg
    15241 ttccattttt cccattacgc catgaggctg taaatctgag gtcgtgatcc ctttgccatc
    15301 ttccagccag gcaccttcca cctgattggc agctgttgcg ccaccccaaa gaaatgtttc
    15361 tggaaatgct ttcataatta actcctttta tcgttagcga atgatggata acagcggctc
    15421 accagcgctt atctgcgccg tgccgtgggg taatacgtct gtaaaatcat cgctattact
    15481 gattaatacc ggcgtcgtca gatcaaatcc ggcctcgcga atagcaggga tatcaaaaga
    15541 aatcagccga tcgcctgtat tgaccttgtc acccacgttg acgtgagcgg aaaagaattt
    15601 gccgtccagt tttacggtgt cgataccgac atgaatcagg atctccacac catcatctga
    15661 ctcaatgcca atagcgtgta atgtggcgaa caacgaagca attcgacccg caaccggaga
    15721 acgcacttca ccaaccgagg gcagaatggc gataccttta cccaacaggc cactggcaaa
    15781 cgtggtatca gcgacgtgaa tgagcggcac aatctctccc gtcatcggtg aacagatacc
    15841 gccctgctca ggtggtgtaa taacctctgg tgttttctct tgcggggcac cctgcgctgg
    15901 ctgacgttta gcggtgatga aatgaagcat caccgtaccg acaaatgcgc aaccgatggc
    15961 aatgacaccg ccaataacgc tggcccagac ggtgaaatca attcccgttg acgggatggt
    16021 ttgcatgaag gtgaaaatac ttggcaaacc aaaggagtac actttcgttt gcgcgtagcc
    16081 aatgatggtg gcccccaaag ccccactgat acaggcgata acaaaggggt acttacgcgg
    16141 cagattaacg ccatataccg ctggttcggt gataccaaac aggcccgtca acgccgctga
    16201 tcccgccacc acttttttct gcgcatcgcg ttcgcagagg aagacgccga gcgccgcccc
    16261 gacctgcgcc ataatggcgg gcattaacag cgggatcatg gtgtcgtagc ccagcacggt
    16321 gaagttattg atacacagcg gcaccaggcc ccagtgcagt ccgaacatga cgaagatttg
    16381 ccagaagccg cccattaccg cgcccgcaaa tgcaggaacc gcctgataaa gccagagata
    16441 accggcggca atcagttcgc ttatccaggt tgatagcggc cccaccagca gaaaggtgac
    16501 gggtgtgata accatcagac atagcaatgg tgtgaagaaa tttttgattg ccgacggtaa
    16561 ccacgcatta agtcggcgtt ccagaatgct gcacaaccag gcagaaaaaa taatgggaat
    16621 aaccgatgac gagtaattca acaatgtgac cggaataccc aggaaatcca gccccagctc
    16681 atccgctttt tgcccgttct cgaaagcagt cagaattaat ggatgcacta acgctccacc
    16741 aatcaccatg gcagtaaatg gattaccgcc gaagcgtttc cccgcggtgt atcccaggat
    16801 tatcgggaag aaccaaaaca aggcatcact ggcgctgaat aaaattaaat aagtaccact
    16861 ttgttcggtc gtccactgaa aagtgagcgc cagagccagc atacctttca ggatcccggt
    16921 tgccgccatc aaaccgatca gaggcgtaaa aatacctgaa ataacataaa caaagcggtt
    16981 tagcagatta cctttctcat cattttccgg tgcctgttgc gctttttcgc caaggcctgc
    17041 cacgctgttg accgccagga agacatcggc cacatggtta cctatgacca cctgaaactg
    17101 gccaccgctt tccaccacca taataatacc gggggtgttt ttcagtacct cagcttgcgc
    17161 tttgctttca tcctttaact taaaacgtaa tcgcgttgcg caatgcatca gactaacaat
    17221 gttatctgcg cccccgactc ctgcgactat ttttctggct aactccgtca taacttgccc
    17281 tctaccgctt tgcggcaaaa ctccaaaaaa aacctgaaaa aaacggcctg acgtgaatca
    17341 agcaattttt ttcaggtttt gcccgcttag tgcggtaaca atcctttact cagtaataat
    17401 atttcagtgt tctttgcgca cgcgctctat atttatggct aaaaacataa tctctgcggg
    17461 tgaaatttta cgttgatact gcaaaccaat aaaaatggcg atccgttccg cacattgcca
    17521 tgcttgcggg taattttgtt ttactgcttg ttgtaatgat tcatcactat cgttaattga
    17581 agcatgttca agaatacgcc aggataaaaa cttcagatgt gtaaccagtc gctgataact
    17641 caagctttct tcctggtaat taaggctgaa ctgaaatttt attaattgca gcatttcgcg
    17701 cattaactgc gtgacacctg caacatcctc catatttccg ctcatttggg cactgaccag
    17761 atgcatggca ataaagccca cttcatcttt cggtaactgc acgcccaacc gtttatcaat
    17821 aatggttaac gcttcttccc ctagctggaa ctctttcggg taaagccgct ggatatccca
    17881 cagcaacggg ttgggcagca acacgttttg ctgaaagcgt ttaatcgcaa actggcaatg
    17941 gtcagttagc gagatataaa tactgtcctg taattttccc aagcgctcct gcgctaaaga
    18001 gataatacga tcacaggttg ccatcacctc aagaggaata tgacttaaga gttcgcttaa
    18061 tcgcccgttc agttcatgac tgctcaaggc atactctttt tctattccac ttgagttaat
    18121 tctttcgcca gcgcgttttt gaaagccaat tcctcgcccc atgacgactt tttcccgctg
    18181 ttgatcatca ataaccacca caacattatt gttgagaatt ttggtgattt gcatgttcat
    18241 agcaaggacc ttttttataa acaaaaaaac ccgacttcac cagtattctc tggttatgtc
    18301 aggttttgcc tgcgaatgca gtaacaatcc agtcatttat taatggtttt tataacgaac
    18361 atccaggttc ggaaattaat ttaattgcgt gcagagaaaa tagcaatgcg ctattgataa
    18421 aaatatgacc atgctcgcag ttattaactt tgtgtaattt taggaattta taaagttata
    18481 tataacaaat cccaataatt aagttattgg gatttgtctg gtgaattatt tgtcgctatc
    18541 ttttcccgcc agcagtttat ccagctcatc accgcctaca tgacggaaat cctgcccctt
    18601 cacgtagtag aagataaact cacaaatatt ctggcagcgg tcgccaatac gttcgataga
    18661 acgcgcgcag aacagcgcag taagtacgct cggaatggta cgcgaatctt ccatcatgta
    18721 ggtcatcagt tgacgaacaa taccttcgta ttcctgatcg acttttttat cttcacgata
    18781 aatacgtacc gcttcgtcaa tgtccatccg cgcgaacgcg tccagcacgt cgtgcagcat
    18841 ctggatggta tgacggccca gcgactccag acttaccagc aacggctgat gctgctggga
    18901 gaatttctcc agcgcagtac ggcagatttt gtccgccacg tcgccaatac gctccagctc
    18961 ggcaatggtt ttactgatca ccataaccag tcgcaggtcg ctcgccgtcg gctgacgttt
    19021 ggcgataatg cgcacgcagg cttcatcgat cgccacttcc atcatgttga cgttcttgtc
    19081 gccttcgatc acgcgcttcg ccagatcgct gtcctggtta tgcatcgcgg tgattgcatc
    19141 agaaagctgc tgctccacca tgccgcccat ggtcatcacc tgcgtgcgga tactttccag
    19201 ttcggcgttg aactggccgg aaatatgttt attaagattg agactgtcca taacgcactc
    19261 ctgaatcaac cgtaacgacc ggtgatgtag tcttctgttt gtttcttcgc tggcttggtg
    19321 aacagatcgt ccgtgttgct gaactcaatc aattcgccca ggtacataaa cgccgtgtgg
    19381 tcggaacaac gcgcagcctg ctgcatgttg tgggtgacga tcaccacggt gtaatcctgc
    19441 ttcagttcgg tgatcagctc ttcaatacgc ccggtagaga tagggtcgag cgccgaacac
    19501 ggttcgtcga gcagcagcac ttccgggcga atggcgatac cacgcgcaat acacagacgc
    19561 tgttgctgac caccagagag agagtaaccg ctctggtgca atttatcttt ggtttcgttc
    19621 cacaatgcgg ctttggtcaa tgcccactgc acgcgctcgt ccatgtcggc acgggagagc
    19681 ttctcaaaca gacgaacgcc aaaagcgatg ttgtcgtaga tggacatcgg aaacggcgtc
    19741 ggtttctgga acaccatgcc cactttcgca cgcagcaggg cgatatcctg agagttggtc
    19801 aggatgttgt cgccatcaag cagaatttca ccttccgcac gctgctccgg gtacagttca
    19861 aacattttgt tgaaggtacg cagcagcgtc gatttaccgc agccggacgg cccgataaac
    19921 gccgttacct ggtttttagc gatatccagg ttgatgtttt tcagggcatg gaatttgccg
    19981 tagtagaagt tcaaattacg aacctgaatt ttactcgggg cagtttcaac catactcatt
    20041 gcaatctttt cctcatcgtg cctgatgcac ttcgcgaatc aggcttaccg taggccggat
    20101 aaggcgttca cgccgcatcc ggcaaagtgt taaccgtgtt tattcttcgc aaaaacaacg
    20161 cgcgccagaa tgttcagcag cagtacgcac agggtaataa tcaacacccc ggcccaggcc
    20221 agttgctgcc attctgcaaa cgggctcatc gcaaacttaa agatcgtcac cggcaggttg
    20281 gcgatcggct gcatcatgtc cgtgctccag aactggttgg agagcgcggt aaacagcagc
    20341 ggcgcggttt caccggcaat acgggcaatc gccagcagga taccggtcat gatcccggac
    20401 accgacgctt tcagcgtaat cgcggagatc attttccact tcggtgtacc cagcgcataa
    20461 gccgcttcac gcaggctgtc cggcaccagt ttcagcatgt tctcggtggt gcggataaca
    20521 atcggcacct gcaacagcgc cagcgcaatt acacccgccc agccagagaa gtgctccatc
    20581 tgcgccacca cgatggtgta aacaaacaga ccaaccacaa tcgacggcgc ggagagcaga
    20641 atgtcgttaa tgaagcgaat cacttctgcc agccaggatt tacgaccata ttccgccaga
    20701 taaatccccg ccataatgcc cagcggcgta ccgaataccg tggcccacaa aattaacagc
    20761 ccgctacccg ccagggcgtt cgccagacca ccaccttccg tattgggcgg cggcgtcatt
    20821 tcagtgaaca gcgccagcga cataccgtcg ataccgcgag tgatggtgga cattaaaatc
    20881 cagatcagcc agaacaggcc gaaggccatc gtcgccatcg agagcgtcag cgcaatacgg
    20941 tttttgaggc ggcgacgcgc ctgcattttg cggcgagatt cagccagcgc cgcagtggtt
    21001 tgcatttcaa ccatagccat tagcgtgccc cctcattctt agccaggcgc ataatcataa
    21061 acttcgatgc ggcgaggacg atgaaggtaa tcacaaacag gatcaggccc agttccatca
    21121 gtgcggcaac gtgcagaccg gattccgctt ccgcaaattc gttcgccagc gcagaggtga
    21181 tactgttgcc cggcatatac agcgaggcgc tgtcgagctg gtaggtgtta ccgatgataa
    21241 aggtcaccgc catggtttca ccgagcgcgc ggcccagccc cagcatgatg ccgccgataa
    21301 caccattttt ggtgaacgga agaacgatac gccagataac ttcccaggtg gtgcagccaa
    21361 taccgtaggc cgactctttc atcatcaccg gggtttgttc gaacacatca cgcattaccg
    21421 ccgcaatgta cggaataatc atgatggcga ggatcacgcc tgccgcgagg ataccgatac
    21481 caaatgcggg gccagagaac agcgcgccaa caatcgggat attcgacatg atattgccga
    21541 ccggctcctg aaagtaaacg gcgaacagcg gcgcaaagat aaacaggccc cacatgccgt
    21601 aaacgatact tggaatggct gccagcagct caatggcgat acccagcggg cgtttcagcc
    21661 agccaggcgc aagctcagtc aggaacaggg cgataccgaa actcaccggg acggcgatca
    21721 gcagcgcgat aaacgaagtc accaacgtac cgtagatcgg caccagcgcc ccgtagatat
    21781 cgttcggtgc atcccactct ttggtccata ggaaagccag accaaatttc tgaatgctcg
    21841 gccaggagga gatgatcaga gagacaataa tgccacccaa catcaatagc acaatcagcg
    21901 ccgccagttt taccagcacg ctgaaaatta tgtcgccctt tttacccggt gggttaaaag
    21961 caggcttggt tgcagccata agttactctt cagttaaacg cgtttacaaa gtcattttgt
    22021 aatgccggat gcggcgtaaa acaccgtacc cggcctggag ttttattagt acagcggctt
    22081 accgctactg tctttaatat tggtcttcca cgcagcgcga acctgttcaa ctacgctatc
    22141 cggcaggctg gcgtaatcca ggtcgttcgc ctgtttagcc ccggttttgt aagcccagtc
    22201 gaagaacttc agcacttctg tgccttgttc tggtttcttc tgatctttgt ggatcagaat
    22261 gaacgtggta gaggtaatag gccatgcatc ttcgcctttc tggttggtca gatcctgagc
    22321 gaaggttttg ctccagtctg caccttttgc tgcattagcg aagttttctt cggtcggact
    22381 aaccggttta ccatcagcgg agatcagttt ggtgtacgcc aggttgttct gcttcgcgta
    22441 agcatattca acataaccaa ttgcacccgg cagacgctga acgaacgcgg cgataccgtc
    22501 gttaccttta ccgcccagac cgatcggcca ttttacggta gagccagtac caacgttgtt
    22561 tttccactct tcgttcactt tcgccaggta gctggtgaag acgaaggaag tcccggagcc
    22621 atctgcgcgg cgtactacag caatgttttg tgaaggcagt ttcagacccg gattcagttt
    22681 ggcgatggct tcatcatccc acttcttgat tttgcccagg tagatgtcgc cgagggtttt
    22741 accatccagc accagttcgc cagacttcag ccctggaatg ttaaccgcca gcaccacgcc
    22801 gccaatcacg gtcgggaact ggaacagacc ttcctgagcc agtttttcgt cagacagcgg
    22861 cgcgtcagag gcaccaaaat caacggtatt agcgataatc tgttttacgc caccggaaga
    22921 accgataccc tggtagttaa ctttattacc ggtttctttc tggtaagtgt cagcccattt
    22981 ggcatacacc ggcgcaggga aggttgcacc tgcacctgtc aggcttgctt ctgcaaacac
    23041 agagaaagcg ctcatcgata aggtcgcggc gacaacagtt gcgacggtgg tacgcataac
    23101 tttcataatg tctcctggga ggattcataa agcattgttt gttggctacg agaagcaaaa
    23161 taggacaaac aggtgacagt tatatgtaag gaatatgaca tttttatgac agaaaggaat
    23221 taccaaataa cttactgatt ttaaatgtga agatgaaaat cccacatgtc atctttttgt
    23281 catgatgcat ttaaaagtga taaagaaatg ggtgagatga caacgatgtg atgaacaatc
    23341 gttgtcagac ctattagcgc acaatctcaa cagttatcgt cgctgttgca gtaaattgcc
    23401 ccggggctgg gcgagctcca gtaaggctcg caggagcagc tgaaaaagtt acactacctg
    23461 atttttcacc atacactgtt gatttaccca tgtctgttgg caactcacca ccgttcactt
    23521 tgatttcctt attatttttg tcgtaaattt ttatccccac gttagagttt gaagttacga
    23581 ccattgaatt gtccgtattg ggttcggtag cataaaaaga cgtactgata tcatcggtat
    23641 tcagtatccc caggttttca cactcaatag tgaccgtttt agtaatttta tgtgttgtta
    23701 ccgcacttcc aacaacagat gaaaaatctg cagctggtat ttctttaaaa tcaaaataaa
    23761 ctggctgtaa attatttatt tcgcaacttt gcggagctgt aatatcgccc cgaatataaa
    23821 catcagccat cggctgtgag taatccaccg gggtgtcacg gctaattgtt ccatacaatg
    23881 tcactatct
//
LOCUS       DAZGVQ010000074        23651 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000074 DAZGVQ010000000
VERSION     DAZGVQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.074"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..787
                     /locus_tag="ACHLAG_002787"
     CDS             <1..787
                     /locus_tag="ACHLAG_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFP0655983.1"
                     /translation="FETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQK
                     MHNKAQKGGETCIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRI
                     GELATVNPGTDLTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKA
                     LTVNGEWQADEYGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAAD
                     HYTVNAWPSIAKGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            812..3241
                     /gene="torZ"
                     /locus_tag="ACHLAG_002788"
     CDS             812..3241
                     /gene="torZ"
                     /locus_tag="ACHLAG_002788"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFP0655984.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAIQA"
     gene            complement(3406..4377)
                     /gene="cmoB"
                     /locus_tag="ACHLAG_002789"
     CDS             complement(3406..4377)
                     /gene="cmoB"
                     /locus_tag="ACHLAG_002789"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFP0655985.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(4374..5117)
                     /gene="cmoA"
                     /locus_tag="ACHLAG_002790"
     CDS             complement(4374..5117)
                     /gene="cmoA"
                     /locus_tag="ACHLAG_002790"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFP0655986.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLDPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(5158..5553)
                     /gene="yecN"
                     /locus_tag="ACHLAG_002791"
     CDS             complement(5158..5553)
                     /gene="yecN"
                     /locus_tag="ACHLAG_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFP0655987.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            complement(5606..6403)
                     /gene="yecE"
                     /locus_tag="ACHLAG_002792"
     CDS             complement(5606..6403)
                     /gene="yecE"
                     /locus_tag="ACHLAG_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFP0655988.1"
                     /translation="MEPDSEIISLNISYFNGSCRHINCVEGNTTLYALPKPEVVLRWR
                     EQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQLPATFGPR
                     ELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVILDSRPVHA
                     ACPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQKLAQWHQ
                     TTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            complement(6387..7673)
                     /locus_tag="ACHLAG_002793"
     CDS             complement(6387..7673)
                     /locus_tag="ACHLAG_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0655989.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEIGSN
                     AFSRYQSVVRNMLPRIGEKRLASSVTKEDLLFIRKDLLTGYHNLSNGKTTPIKGRSVV
                     TVNYYMTTIAGMFQFAADNGYIVSNPFNGLTPLKRSRTEPDPLTRDEFIRFIDACHHQ
                     QTKNLWSLAVYTGIRHGELIALAWEDIDLKAKTMTIRRNYTKLGEFTLPKTEAGTDRV
                     IHLVQPAVDALKSQAEMTRFDQQYQIDVKLREFGRSTSHECTFVFNPQLVKKCQHVGH
                     HYKADSIRDSWASALRRAGLRHRKAYQSRHTYACWALSAGANPSFIANQMGHANAQMV
                     FNVYGAWMEDNNIGQIELLNKQLTESVPYMPHRARL"
     gene            complement(7707..7961)
                     /gene="xisR"
                     /locus_tag="ACHLAG_002794"
     CDS             complement(7707..7961)
                     /gene="xisR"
                     /locus_tag="ACHLAG_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP0655990.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            complement(8028..8114)
                     /locus_tag="ACHLAG_002795"
                     /pseudo
     CDS             complement(8028..8114)
                     /locus_tag="ACHLAG_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8118..8360)
                     /locus_tag="ACHLAG_002796"
     CDS             complement(8118..8360)
                     /locus_tag="ACHLAG_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0655991.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRITEHSVFFRRMDGRSGRVRIG
                     ELNCLFEHIDHQEYRKILADTEQEKHLKKLRAIKRK"
     gene            complement(8445..9224)
                     /locus_tag="ACHLAG_002797"
     CDS             complement(8445..9224)
                     /locus_tag="ACHLAG_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP0655992.1"
                     /translation="MHRVCLEANRYLDKYDAMAKEVNKLLGRIAPPAPVFNGEYGDAY
                     QGAREDLSIWKRRALEADEHVRRLEQINDHMVKEAQGESRMGEPVIREPAPVVPEEAT
                     PENVEMLSGYVSTYKLTDSERDIAAEIWNACRAAMLQSGNFRENKNSSTNNFREIAET
                     STNYPAIPSEVLSAILKVARIRADFDDFDGDRRGIGDCLDEAEQELIVTINKYASQLA
                     AEPIAPNDVREQTAIPQVPVTPDGWISCSERMPDDRQEVNQ"
     gene            complement(9255..9389)
                     /locus_tag="ACHLAG_002798"
                     /pseudo
     CDS             complement(9255..9389)
                     /locus_tag="ACHLAG_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309545.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9723..9901)
                     /locus_tag="ACHLAG_002799"
                     /pseudo
     CDS             complement(9723..9901)
                     /locus_tag="ACHLAG_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9903..10475)
                     /locus_tag="ACHLAG_002800"
     CDS             complement(9903..10475)
                     /locus_tag="ACHLAG_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP0655993.1"
                     /translation="MSKIDYQALRKAAQLATQGEWVAFISSGTGTYAVHTPGDKRCED
                     VIKWTGFDGQNNAENNARYIAAFNPEVVQALLDERERNQQYIKRRDQENEDIALTVGK
                     LRVELEAAKSKLNEQREYYEGVISDGCKRIAELEAREVQLPTRYDLRYGHPINADERQ
                     VMIPKENGSWLYLIDLEHALRVADIRIKGE"
     gene            complement(10472..10711)
                     /locus_tag="ACHLAG_002801"
     CDS             complement(10472..10711)
                     /locus_tag="ACHLAG_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655994.1"
                     /translation="MFKQNEKAIAQIADYIPRACRGMQLQEAKARLEKKIALYIDDGC
                     DAAVLNAAFAPALNSHTRESFFSCIAAQIRKGGNQ"
     gene            complement(10704..10907)
                     /locus_tag="ACHLAG_002802"
     CDS             complement(10704..10907)
                     /locus_tag="ACHLAG_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019705292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0655995.1"
                     /translation="MTIVKTHTGTVITKDGPQVKKLHQTERMWVVGKNEFYHKETGRR
                     HFAENTRRRLLLDTIKPIEVKHV"
     gene            complement(10904..11782)
                     /locus_tag="ACHLAG_002803"
     CDS             complement(10904..11782)
                     /locus_tag="ACHLAG_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HFP0655996.1"
                     /translation="MCITHVVSFSGGRTSAYLVHLMEEQRKAGNNVCYIFMDTGCEHP
                     LTYRFIREVVKFWGIPLTVLQVDINPELGQPNGYTEWEPKDIQTRMPVLKPFMDMVKK
                     YGTPYIGGAFCTDRLKLTPFTKYCDDHFGRGNYITWLGIRADEHRRLKPKPGVRYLAE
                     LSDFDKSDVIRWWRKQPFDLQIPEHLGNCVFCIKKSTQKLGIACKDEPGLMRVFNELV
                     TGKHVRDGHRRTGKDVMYRGHLTLDRIARMYADSDYRSLYQAMVLAKRFDTGSCSESC
                     EIWGDQLELKFEEVVA"
     gene            complement(11773..12309)
                     /gene="yfdR"
                     /locus_tag="ACHLAG_002804"
     CDS             complement(11773..12309)
                     /gene="yfdR"
                     /locus_tag="ACHLAG_002804"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP0655997.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            complement(12438..13262)
                     /gene="yfdQ"
                     /locus_tag="ACHLAG_002805"
     CDS             complement(12438..13262)
                     /gene="yfdQ"
                     /locus_tag="ACHLAG_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFP0655998.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAQFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGDAIQATK
                     AAAAVRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            complement(13328..13690)
                     /gene="yfdP"
                     /locus_tag="ACHLAG_002806"
     CDS             complement(13328..13690)
                     /gene="yfdP"
                     /locus_tag="ACHLAG_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFP0655999.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            complement(14414..15106)
                     /locus_tag="ACHLAG_002807"
     CDS             complement(14414..15106)
                     /locus_tag="ACHLAG_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP0656000.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQALSNIARSLGVDIADLFTSDLKSNTVCKNSISEDVAQVKDVFRIEMLDVSASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDIS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            15204..15464
                     /locus_tag="ACHLAG_002808"
     CDS             15204..15464
                     /locus_tag="ACHLAG_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0656001.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIDL
                     AERLANFFKGEISEMQILYPARFQSSQNQNGFKPQEQEVSRG"
     gene            15457..16014
                     /gene="ymfL"
                     /locus_tag="ACHLAG_002809"
     CDS             15457..16014
                     /gene="ymfL"
                     /locus_tag="ACHLAG_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFP0656002.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAAGTHYIADAVAQSAGGVFVSLPEIEAVENADINQR
                     LLEVIEQIGSYSRQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAALVYKIFCAPE
                     NSNARECAAPGVVASIASGCGETNA"
     gene            16160..16339
                     /locus_tag="ACHLAG_002810"
     CDS             16160..16339
                     /locus_tag="ACHLAG_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0656003.1"
                     /translation="MQELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYPHECFSPLW
                     KFRRDFVECEGPPAY"
     gene            16269..17171
                     /locus_tag="ACHLAG_002811"
     CDS             16269..17171
                     /locus_tag="ACHLAG_002811"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP0656004.1"
                     /translation="MSALVLCGNSGVILLSVKDHQHIDSAIPGRYTVQAPYKAGAGIG
                     VLKLSTAMYDAPASFLSSAFAHIQIMVGWAGAPKGAPVSVDAGYANPVQFTTSEIGVS
                     GGGSFSLSTEAAIMATNPALTQPEITIDNGQAVTTSLAVANSFSKRHDDVLKKIRTLD
                     CSPEFCARNFAETSISVNQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYIN
                     AFNQMEKLLSKPSTLSDAADNASVLYSHLSVIHKVWLQQLYPMLAKAESPLAVSLYDR
                     INDAALLASLINLSLNPSEVRGRK"
     gene            17168..17392
                     /locus_tag="ACHLAG_002812"
     CDS             17168..17392
                     /locus_tag="ACHLAG_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656005.1"
                     /translation="MIRNIFKRFTNQTFRCPRPGQWYTTPAGHVLRVSLVDRECQKVI
                     CEPLGRNYRISMPLIAFCSGKMFKRLGGVA"
     gene            17389..17688
                     /locus_tag="ACHLAG_002813"
     CDS             17389..17688
                     /locus_tag="ACHLAG_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0656006.1"
                     /translation="MNCFQFVCGCAFDNPIQRLIMLRVLMSGSSDGEGERVIDHQVLA
                     DFCCCSKQAIFRETLALERAGYLHIRKIATLTIDAKARLQPARGYTILMLRKEVV"
     gene            18276..18668
                     /locus_tag="ACHLAG_002814"
                     /pseudo
     CDS             18276..18668
                     /locus_tag="ACHLAG_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18665..19159
                     /locus_tag="ACHLAG_002815"
     CDS             18665..19159
                     /locus_tag="ACHLAG_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0656007.1"
                     /translation="MIMSLMNDVQKFIEAHPGCTSGDIADAFAGYSRQCVLQSASKLR
                     QSGRVAHCCEGKTRRHFPHQAEISQEEKLQPVLETIPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSRKRAVAGDEWYLSGN
                     YVGA"
     gene            19159..19812
                     /locus_tag="ACHLAG_002816"
     CDS             19159..19812
                     /locus_tag="ACHLAG_002816"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFP0656008.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGAAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            19809..20135
                     /locus_tag="ACHLAG_002817"
     CDS             19809..20135
                     /locus_tag="ACHLAG_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFP0656009.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEANARLRAAHWLH
                     ERGLKV"
     gene            20132..20527
                     /locus_tag="ACHLAG_002818"
     CDS             20132..20527
                     /locus_tag="ACHLAG_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0656010.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKASCKLATR"
     gene            20690..21505
                     /locus_tag="ACHLAG_002819"
     CDS             20690..21505
                     /locus_tag="ACHLAG_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFP0656011.1"
                     /translation="MNQLLVIDGVSVRQYFESNYCLNDLQKAALLAAGENRSSRSLEA
                     HEFMRRPETKALVELLEEETTGDSRSIPVITIQGRNGGTYVCKELVYAYAMWISPAFS
                     LKVIRTFDALHNSSPEETTSDKIKSGVILLESAAKTLNLSNSSKLGAYQKLSKVAGLP
                     ELMPIYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYS
                     RTAINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            21521..22036
                     /locus_tag="ACHLAG_002820"
     CDS             21521..22036
                     /locus_tag="ACHLAG_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656012.1"
                     /translation="MRITPPHLQPVLSRVKRFVERMPEGATLTQISQKVQAYSLLNKR
                     DKEILIGIIRDSGLLVVANDGRTTTLHHPKFGHQAVNSEIPVKTEEPVVIKKTVTPDE
                     LRRHAEELIRAAEEAEKKFNDRAEIKKQLDPLKLEILQAYGMASRKFDEFVDAMADMG
                     KAVQKLKQIVL"
     gene            22046..23035
                     /locus_tag="ACHLAG_002821"
     CDS             22046..23035
                     /locus_tag="ACHLAG_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0656013.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNLLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKVLRLPKTVVPSVTRESDLVPSVPATSIIRDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            23050..23445
                     /locus_tag="ACHLAG_002822"
     CDS             23050..23445
                     /locus_tag="ACHLAG_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP0656014.1"
                     /translation="MMRDIQMVLERWGAWAASGNAGVDYSPIAAGFKGLLPSTAKPRP
                     ACSDDDGLIIENCLTRLKKKKPDEYSLLVAHYLLRISKRQIARTRKKSEKAIRIEMQI
                     AEGFIDGCLSMLGVRLEMDDWLPKKVKND"
BASE COUNT         5971 a         5774 c         5935 g         5971 t
ORIGIN      
        1 gttcgaaact catcgccagg aaatggccga aacagtatgg aaagaattaa aagcaactga
       61 ctctgcaacg tgccgtagtt gccattcttt tgatgccatg gatattgcct cgcaaagtga
      121 atctgcgcag aaaatgcata acaaagcaca aaagggcggc gaaacctgta tcgattgtca
      181 taaaggcatt gcccattttc cgccagaaat aaaaatggat gacaacgcgg cgcatgagct
      241 ggaaagtcag gccgctactt cagtgactaa tggcgcacat atttatcctt tcaaaacttc
      301 tcgcataggc gagctggcta ccgtgaatcc tggtaccgat ctcaccgtcg ttgatgccag
      361 tggcaaacag ccgatcgttc tgttgcaggg ttatcaaatg cagggcagtg aaaacacgct
      421 ctacctggcg gcaggtcaac ggctggcgct agccacatta agtgaagaag gtatcaaggc
      481 gctcacggta aacggggaat ggcaggctga cgaatacggc aatcaatggc gtcaggcgtc
      541 tttacagggt gcgcttaccg atcccgcatt agcggaccgt aaaccgctat ggcaatacgc
      601 tgaaaaactt gacgatacct attgcgctgg ttgccatgcg cctattgccg ctgaccatta
      661 caccgtcaat gcatggccgt ccattgccaa aggaatgggt gcgcgaacca gcatgagcga
      721 aaacgaactg gacattttaa cgcggtattt ccagtacaac gccaaagata ttaccgagaa
      781 acagtgatcc tcgttgatca gggaggaagt tatgacatta acaagacgtg aatttattaa
      841 acacagcggt attgccgctg gagcgctggt ggtgacatcg gcagcaccgc tccccgcatg
      901 ggcagaagag aagggcggta aaatcctcac cgcgggacgt tggggagcga tgaacgtaga
      961 agtgaaggac ggcaagattg tttcttcaac aggcgcgctg gcgaaaacca taccgaactc
     1021 cttacagtct acggcggcgg atcaggtaca caccacggca cgtattcagc atccgatggt
     1081 aagaaaaagc tatctcgata atccactgca accggcgaaa ggtcgtggcg aagatactta
     1141 tgtacaggtg agctgggagc aagcattaaa actgattcat gagcaacacg atcgcattcg
     1201 caaagctaac ggaccatctg ctatttttgc cggatcatac ggctggcgct cttctggcgt
     1261 gttgcataaa gcgcaaacct tactgcaacg ttatatgaac ctggcgggcg gttattccgg
     1321 gcatagcggc gattattcaa ccggggcagc acaggtgatc atgccgcatg tggtcggttc
     1381 tgtagaggtg tatgaacagc agacctcctg gccgctgatt ctggaaaaca gccaggctgt
     1441 ggtgctgtgg gggatgaacc cgctgaatac cttaaaaatt gcctggagca gtaccgatga
     1501 gcaagggctg gaatacttcc atcagctgaa aaaatctggc aaaccagtga ttgccattga
     1561 tcctatccgc tccgaaacca tcgagttctt tggtgataat gctacctgga tcgcgccgaa
     1621 tatgggcacc gacgtggcac tgatgttagg gattgcgcat accctgatga cacaaggtaa
     1681 acacgataaa gtgttccttg aaaaatacac caccgggtat ccgcagtttg aagagtatct
     1741 gacaggtaag agcgataata ctccgaagag cgcggcctgg gcggcggaaa ttactggcgt
     1801 accggaagcg cagatagtca aactcgctga actgatggcg gcgaatcgca caatgctgat
     1861 ggctggctgg ggaattcagc gccagcaata tggtgaacag aaacactgga tgctggtgac
     1921 gctggcggcg atgttagggc agattggtac accgggcggc ggttttggtt tctcttatca
     1981 ttactccaac ggtggcaatc cgacgcgtgt tggcggcgtg ctgccggaaa tgtctgcggc
     2041 gattgccgga caggccagcg aggccgctga tgatggcgga atgacggcga ttcctgttgc
     2101 ccgtatcgtc gatgcgctgg aaaatcctgg tggcaaatat cagcataacg gtaaagaaca
     2161 aacgtatccg aatatcaaaa tgatctggtg ggcgggtggc ggtaacttta cccatcacca
     2221 ggatactaat cgtctgatca aagcatggca gaaaccggag atgatcgtcg tttctgaatg
     2281 ctactggacc gcagctgcta aacatgcaga tatcgtatta ccgatcacca catcgtttga
     2341 gcgcaatgac ttgacgatga ccggtgatta cagcaaccag catattgtgc cgatgaagca
     2401 ggctgttgct ccgcaatttg aagcgcgtaa cgattttgac gtatttgccg atcttgctga
     2461 attactgaaa cctggcggaa aagagatcta taccgaaggt aaagatgaaa tggcgtggct
     2521 gaaatttttc tatgatgccg ctcagaaagg tgctcgtgcg caacgcgtga ctatgccgat
     2581 gtttaatgcc ttctggcagc aaaataaact gatcgaaatg cgccgcagcg agaagaacga
     2641 acagtacgtt cgttatgctg atttccgcgc cgatccggtg aaaaatgcgc tgggtacgcc
     2701 aagcggcaaa attgagattt actccaaaac gctggaaaaa tttggctata aggattgccc
     2761 ggcacaccca acctggcttg cgcctgatga gtggaagggt accgccgacg agaagcagtt
     2821 gcagcttctg accgcacatc cggcacaccg tttacatagt cagcttaact atgcggaact
     2881 gcgtaaaaaa tatgcggttg cagatcgtga accaatcact attcacaccg aagatgctgc
     2941 tcgctttggt attgcgaatg gtgatctggt gcgtgtgtgg aacaaacgtg gtcagattct
     3001 gacaggcgcg gtggtgactg acgggatcaa aaaaggcgtg gtatgcgtgc atgaaggtgc
     3061 atggccagat ctggaaaatg gcttgtgtaa aaacggcagt gcgaacgtgt taacggcgga
     3121 tatccccagc tcgcagctgg caaatgcctg tgccggtaac tctgcgctgg tgtatatcga
     3181 aaaatatacg ggcaatgcgc cgaagttaac ggcgtttgat cagccagcta ttcaggcata
     3241 agaagtgaat agcgcccgga aacgggcgct gaggttatgc aaacgtaaaa ctgcctgatg
     3301 cgctatgctt atcaggccta catagtccct gcaatagatt gaattagcaa gggtttgtag
     3361 gccggataag gcattcaagc cgcatccggc agtaatgcca gacctttacg gcttgcgcgc
     3421 aatcagcacc gcgcgtttag gcgcaggata accttcaact gtcttactcg gatcatgtgg
     3481 gtcgaggaaa tcggacagag attcggtaac catccattca gtgcgtcgct gctcttcagt
     3541 agtggtaacg cacacatcca caacgcggat atctacaaaa ccacacttct tcagccagtt
     3601 tttcagcgcc agcgcggaag ggatgaaata gacattacgc atttgcgcgt aacgatcgcc
     3661 tggtaccagc accgtgttct catcgccatc aataaccagc gtttccagca ccagttcacc
     3721 ctcattcacc agttgatctt tcaactgcca gagatgctcc agcggcgaac ggcgatggta
     3781 aagcacgccc atcgaaaaga cggtatcaaa ggctttcagt gccggaagct gttcaatacc
     3841 taacggtaac aaatgtgcgc gctgatcgtt acccagtagt ttacgcaccg cttcaaactg
     3901 gcacagaaac agttgcgtag ggtcgatccc caccgcgagg tgcgcgcctg cgccaatcat
     3961 gcgccacatg tgataaccgc tgccgcagcc gacatccaga atggtgcgtc cggttaaatc
     4021 agaaagatgg ggcagaacac gatcccattt ccagtcggaa cgccattcgg tatcgatgtt
     4081 gacgccatac agtgagaacg gccctttgcg ccatggcatc aggttccgca taagcgtttc
     4141 aatgcgttta atttgcccgg tgctcagtgg ctcttcgctt tcggcggtta cgctatgcaa
     4201 taaatccaga cgatacggtt taatttcagg cagaaattcc accgcgttgg accactgctt
     4261 aaacagcccg tgctgctgct cgcgctgcca gttagcaatc tgcgcgggca gcgtttcgag
     4321 ccagtgtgaa agatgatttt tggcaatcag agaataaaag ttaccaaagt cgatcatgca
     4381 gcgtcctctg cttttaatgc caccagtgaa ccaaagttaa agcactggaa ccacagctcg
     4441 ctatgctcaa aaccggcttt atgcaggcgt gctttatggg tttccacgga atcggtcagc
     4501 atcacgtttt ccagcatgct gcgcttctgg ctgatctcca gttcgctgta accgttggca
     4561 cgtttaaagt cgtggtgcat gttgaacaac agttcaccaa ctttggcatc ttcgaaactg
     4621 aatttttccg aaagcaccag cgcgccgcct gggttcagtc cttgataaat tttatccagt
     4681 aatgcctggc gctcggaagg atccaggaat tgcagggtaa aattcagcac caccatcgat
     4741 gcgttttcaa tggcgatatc gcgaatatca ccttcaatga cgtctactgg cgtaggggct
     4801 ttataggcgt caatatgacg acggcagcgt tcaatcatcg ccggggagtt gtcgatggca
     4861 ataattttgc aattatcatg atgaatgttg cgacgcaccg agagcgtcgc cgcgcccaga
     4921 gaacaaccca gatcgtaaac ctgcgtacca ggttgaacga agcgctcggc taacatacca
     4981 atcatggaaa taatattgga atagccggga acggaacgct ggatcatatc cgggaagact
     5041 tcagctaccc gttcatcaaa ggtccagtcg cccagtctgg cgataggggc agaaaatagc
     5101 gtgtcgcggt gagacataac gtaaaaatcc gggaaaaaga aagtggcgta ttgtgcgcta
     5161 acgcagggag aaaaccaact cccagggcat ataccaaaga ttcgccagca ccatcagcaa
     5221 cagcgcacac caggtggcgc tcatgccgga acgtcgccag cggaacagac ggtgatgaaa
     5281 accgtaataa tgcatcagac gaccagcaag caaaacgatg ccgcaaatat gcaccatcca
     5341 ggtttctgcg ccattcattt ccataaacag catcaacaca atcgcgatgg gaatatattc
     5401 caccgcgtta ccatgaatgc gaatagcgct ttgcagttcg ctaaaaccgc cgtcgccata
     5461 ggcaacgcgg tactgcaaac gcaagcgaac gacatcaaaa gagaacttca ttaataacaa
     5521 cgcacttaaa acggcataca gcgcgcttac catacaaact ccctttaaaa tggccgattg
     5581 cacctgtcta tgataggtgg caaattcaga aaagagaaga ttgctgtgga atagccggaa
     5641 ctgctccgat ctctggaagc gttttacgta agtcttccca cagggtatgt accagttccg
     5701 gggcctgggc aatatctggc gtatgtaaaa aaagataagg cgtagtggtc tgatgccact
     5761 gcgctaattt ctgtaaccag acctgaaata attcccggtt ttgcgtcata tcatcactac
     5821 cgataaaacg aatcagcgga tttgtcgccg tcagtacagc atgtaccgga actttaggtt
     5881 tttttcgttg agcgtcgcga atagcttcac tgtgtggaca tgctgcatga accgggcggc
     5941 tgtctaaaat cacccgatta acgccgcgct gatgtaaacc gcgattaagc gtttgttcct
     6001 cttccccttt ggcgaaaaac tgtggatggc ggacttccac accataatta aattcgccag
     6061 ggagagaatc gagaaaatgc caaagcgcag gcagctcccg tgggccgaat gtggcaggca
     6121 gttgcagcca gtattgccca atgcgcggag ccaacggtga catgcgggtc aaaaattcag
     6181 tcactaaatc atcgcaatgc cgtaatgctg cctgatgcga aatggtcgcc ggaaacttaa
     6241 aacagaagcg gaagtcatct gtggtctgct cacgccagcg caggacaacc tcgggtttcg
     6301 gcagggcgta aagcgtggtg ttgccctcca cgcagttaat gtgacggcaa ctaccattaa
     6361 aataactgat atttaaagat attatttcag agtctggctc tatggggcat gtatgggaca
     6421 ctctctgtca actgcttatt gagtagttct atttgcccga tattgttatc ttccatccat
     6481 gcaccgtaaa cgttgaatac catttgtgca tttgcatggc ccatttggtt tgctatgaag
     6541 cttggattcg ctcctgccga taatgcccag caggcataag tatgcctgga ctgataggct
     6601 tttctgtgcc gcagtcctgc tcgccttaat gcagatgccc aggaatctct gatggaatct
     6661 gctttatagt ggtggccgac gtgctggcat tttttcacca gttgcggatt aaaaacaaac
     6721 gtgcattcat ggcttgtgct gcgtccgaac tcccgcagtt tgacatcaat ttgatattgc
     6781 tggtcaaacc ttgtcatttc agcctggctt ttcagggcat caacagctgg ttgaacaaga
     6841 tgaataacac gatcagttcc cgcctctgtt tttggtagag tgaactcccc gagttttgta
     6901 taattacgac ggatagtcat tgttttagct tttaaatcaa tatcctccca ggcgagagct
     6961 attagctcac cgtgacgaat gcctgtgtat actgctaagg accacaggtt tttcgtttgt
     7021 tgatggtgac aggcatcaat aaagcgaata aattcgtcac gcgtgagcgg atctggctct
     7081 gttctggatc tctttaatgg tgtcagcccg ttaaatgggt ttgacacgat atagccgtta
     7141 tcagccgcaa attgaaacat tcctgcaatt gtcgtcatgt agtaattaac cgtaactact
     7201 gaccttcctt taatcggcgt tgtttttccg ttagagagat tatgataccc ggttaacaag
     7261 tctttcctga taaacagtaa atcttccttt gttaccgacg aagcaagacg tttttcccct
     7321 atgcgaggaa gcatgtttct caccacggat tgataccgac tgaatgcatt acttcctatc
     7381 tcaattttct taaggtccag ccatttttcc gaaagtgcct taacggttat ctctcttttt
     7441 cccagaccaa agtgtttcag gttaggggaa ttagggaact gcgcggcgta gtcgaaactc
     7501 cccattctga ttgcaaaaca aacggaagtg cgaagttcac cagcgatctt ccggtttttg
     7561 gctgtgtcag gaacaccgag gttttctctg acacgtttgc cattatagtg aaaccatata
     7621 cggagtgatc ctccatggtt ttcaacgcct gtcgggtatg atgcgttact cattaaacct
     7681 cccagacgtc caggagcatt aacaggttaa ccggaacttg catttttggc acctggttgt
     7741 ttctggtttt cgatccatcg cataatttct tcgatgttgt acaggcattc actgtagtgc
     7801 cccggatcac cttctacagc gtaatggcgg tattcttttc cctgcatcca tgactttctt
     7861 cttgcccgct caatggtgcc aggctttagc cctgttgatg caatgaggac tctctccgta
     7921 caccatttgc tgggggttat ctgatagatg attgtctgca tgccaacctc ataaaacttt
     7981 catccacggc agtggcacca cacttcaaac attcgcttca caacttaacg acagtagaag
     8041 ccgtcaacat ctcgcgtcag gtcatagcga ttgccgtaac gctggtggac ccatcgttca
     8101 aatgctttat tcattcttta cttccttttt atggctcgta attttttcag gtgcttttcc
     8161 tgctcagtgt ccgcgagaat tttgcggtac tcctggtggt caatatgttc gaacaggcag
     8221 tttaactcac caatgcgtac ccgcccggat cgtccgtcca tccgtcgaaa gaacactgag
     8281 tgctcagtga tgcgagtaat caccacgggg tatccagctc tgtccgtgta tatctgaccg
     8341 cgttgaatca aagcgaacat gtggttatcc ccatcgacaa atcgagaaca caacaaacgc
     8401 tgctgcgaat accaccccca gagttacgat tgcatcaggc cagctcattg attcacctcc
     8461 tgcctgtcgt ccggcattcg ctcactacag cttatccaac catccggagt taccggaact
     8521 tgtggaatgg ctgtctgctc tcgaacgtca ttaggcgcta taggttctgc tgccaactga
     8581 ctggcatatt tgttaatggt aacgataagc tcttgctcag cctcatccag acaatcaccg
     8641 atacctcgcc tgtcaccgtc aaaatcatcg aaatcggcac gaatcctggc aaccttcagg
     8701 attgcggaca acacctcact aggaattgcc ggatagttgg ttgacgtttc cgcgatttcc
     8761 cgaaaattat tggttgacga attcttgttt tcccgaaagt ttccggactg aagcatggcg
     8821 gcgcggcagg cgttccatat ttcggcagca atatcgcgct cgctatcggt taatttgtac
     8881 gttgaaacat agccagagag catttctacg ttttccggag ttgcttcttc aggcactacc
     8941 ggtgctggct cacgtattac aggctcgccc atgcgtgatt ctccctgcgc ctctttcacc
     9001 atgtggtcat tgatttgctc cagtcgccga acgtgctcat cagcttcaag cgctcgccgt
     9061 ttccagatgg acaggtcttc acgagcgccc tgatatgcgt caccgtattc gccgttaaat
     9121 actggcgctg gcggggcgat gcgtccaagc aacttattta cctctttcgc catcgcgtca
     9181 tatttatcta aatagcgatt agcttctaag cagactcggt gcatctgatc tgagttaact
     9241 cgtttaactg gatctgcttc caatgatgcc agtgcaatcc gtgccagttc ttccgcttct
     9301 tctgctggca gtacaacgtt gctacccggt ccgtatgttt cgcgccactg cttgattgtc
     9361 agcagtcgcc ctttggtaat agtgatcatg ccgcgtttcc ttctttctta ttaacaatca
     9421 caccgtcata tatttcatta aggtgccctc tcaactccat gcgccttaat gcagataaca
     9481 tgtaatcgca ttcaacctgc ttattcccgg taaatggctt atcgtcagga ttaccccaac
     9541 agcaattacc cctgggccat ccatgtactt tccgtactct tccgttaaca acgtgaagta
     9601 atccccagcc gggaggtaaa tcctcaactg aaataatttc cggctcacta ataaagaatc
     9661 gccagtcgcc catgccaagt gagggatttt tacggaaacg cttttttcta tctgccaaca
     9721 agtcagcacg agaacacttc gcctctatca ggcatgatgc tgaatttctg aatcccatag
     9781 catctggctg ttctccggta ctggttacag caacaaagcg gtcatgaaag caaaccttga
     9841 acccgttgcg cttaaggaac ttgtacgcaa tctgacagag ttcgtggtgt gttaacgcca
     9901 tatcactctc ctttgatgcg aatgtcagcg acgcgtaatg cgtgttctag gtcaatcagg
     9961 taaagccaac tgccattttc tttaggtatc atgacttgtc gctcatctgc atttatcggg
    10021 tgtccatatc gaaggtcgta gcgagtcggt aattgaactt cccgcgcttc cagttcagca
    10081 atacgcttgc acccatcaga gataactccc tcgtaatact cgcgctgctc gttgagtttt
    10141 gattttgctg cttcaagctc aacgcgcagc ttcccaaccg taagcgcaat atcctcgttc
    10201 tcctggtcac ggcgtttgat gtattgctgg tttctttccc gttcatccag cagcgcctgc
    10261 actacttcag ggttgaaagc tgcgatataa cgagcgttgt tctctgcgtt gttctgtcca
    10321 tcaaagccgg tccatttgat aacgtcttca catcgtttat caccaggtgt atgcaccgca
    10381 tatgtaccag taccagacga aataaatgcg acccattcac cctgtgttgc cagttgcgcc
    10441 gctttacgca gtgcctgata gtcaatcttg ctcactggtt gcctcctttg cggatctgcg
    10501 ctgcgatgca cgaaaaaaaa gactcccgcg tatgactgtt aagagctggc gcgaacgctg
    10561 cgttaagaac ggcagcatca cagccgtcat cgatatagag cgcaattttt ttctccaggc
    10621 gcgctttggc ttcctgcaac tgcatacccc ggcacgcacg cgggatataa tcagcaattt
    10681 gagcgatagc tttttcgttc tgtttaaaca tgcttcacct cgataggctt gatggtgtct
    10741 aacagcagtc ggcggcgcgt attttctgca aagtggcggc gtccagtttc tttgtggtaa
    10801 aactcgtttt tgccgacgac ccacatccgt tctgtttggt gcaatttttt tacctgcggt
    10861 ccatctttgg tgatcacggt accggtatgg gtttttacga ttgtcatgcc actacctctt
    10921 cgaatttcaa ctccaattgg tcaccccaga tttcacatga ttcggaacac gaaccagtat
    10981 caaaccgctt ggccagcacc atcgcctgat acaaacttct gtagtcgctg tcggcataca
    11041 ttctggcaat cctgtcaagc gtcaggtgac cacggtacat aacgtcttta cctgttctgc
    11101 gatgaccatc ccggacgtgt ttgcctgtaa ccagttcatt aaaaacccgc atcagaccag
    11161 gttcgtcttt acatgcgatc cccagctttt gcgttgactt tttgatgcag aaaacacagt
    11221 tcccgaggtg ctccgggatt tgcaaatcaa aaggttgttt acgccaccac cggataacat
    11281 ccgacttatc aaaatctgac agctcggcaa gataccggac gcccggtttc ggtttcagcc
    11341 tacggtgttc gtccgcacga atacccagcc atgtgatgta attccctcgc ccaaaatggt
    11401 catcgcaata tttcgtgaaa ggggtgagtt ttagcctgtc agtacagaat gcgccgccga
    11461 tgtatggcgt accgtacttt ttaaccatgt ccataaacgg tttaagcacc ggcattcgcg
    11521 tctgaatatc ctttggttcc cattccgtat aaccatttgg ctgcccaagc tctggattta
    11581 tatcgacctg caacacagtt agcggtatgc cccagaactt cacaacctcc cgaataaagc
    11641 ggtatgtcag cggatgttcg caaccggtat ccataaagat gtagcagacg ttattgccag
    11701 cctttctttg ttcttccatc aggtgaacaa gatatgcaga tgttctcccg ccagaaaaac
    11761 taactacatg agttatgcac atttgcgtaa ttccgataac tcgttgaagc gttccataaa
    11821 catcccgtag gcatggcccg gtgccagtgg aatcactttg aacatctctg ttgccgggat
    11881 accttccaat acaggccaga aagagccatc atcaagtccg agatcgcggc gttcggttgc
    11941 cagcatgatg agatcggcat atttcactgg cgtgctcata acaggaggta acccgtattt
    12001 ctcacggatt acggcgtcta ttttttcttc catccgttta tagtcaggaa gaagtcgttt
    12061 cagtggtgcg gggatgtcct ggcaatacgc ttctgttgca tcatgcatta acgcttcaaa
    12121 agcaaattcc tgcggcacca gctggctgca aagcaccgca tgctgggcga cactgtagaa
    12181 gtgtgaaaga tgtcctgcaa agcgacagat atttgaaagg gaaaccgcga tatcgttaat
    12241 cacgatgtcg tctttattta tcttgtcata ataaaaatgc ttcccggaaa aagttttaat
    12301 aaatgacatt tcgttctcca ctttatatgc gctgcaccgc gctgaattcg ggtaaaagga
    12361 atcccgcacc atccggcgat tattgagtta attacgtttc cataaatgcc cccgcaggag
    12421 catttgcagt aatgaaatca ggcggtgaaa gtaccaataa aggtttctac tttgctgtct
    12481 ttgaatttct caacaagcag atcacgaaat tcgttagcca tttcttcctg cactgcttcc
    12541 agctgaataa tgcgcagaac cagtacagga cgatcgccag tgataatgct gaggcgtaat
    12601 ttaaatggac gttctttcag accttcaaac ggaacgcatt taaattcaaa tgccactggc
    12661 ataatatctt tggtcttcgc ttcgacagac tccatcaggg agcgtttgcc gctgaagtca
    12721 ttgtcttcaa aatcagcagt ctggttcgct tcaattgtga ttttacggac tgccgcagcc
    12781 gcttttgttg cctgaatggc gtcaccatta gcatcaaagc ccacaaggta gtcggcccag
    12841 tcttcaatcc attctgccag tgacttctgg gagttacgct cgccgttaac agacaacaga
    12901 gcagagaact gtgctgtctt tttcagtttg agagtggcgg tgttatctgc gtgacctggt
    12961 tcatcaatag tacccaggtt aagcacactg acggcacgca tattatcggc atcgataaag
    13021 cagcgggtgc cttcatctgc aagatcttta gaataacggg taaagtcatc gatgctggca
    13081 gtggaaagcg caccacggaa acggaagcga tttaaattaa atttttccag atcatgaatg
    13141 cggaaatttt caggtaatgc cactgcgtcg gcaccaatct tactgataat ttcattaaca
    13201 ccctgagcag aaataagggc atggatttga ttaattgcgg ttgcgtctaa gttctgagac
    13261 ataataagtc ctcactatat taagatattc agtgatgaga taaataatca gttaattaag
    13321 aacgatatta atgacctgct gcgcggagtt ttccgtcagg ctcaccggca agagtcagta
    13381 attgtccctg gtcttcctgc agaatagtca ggcgaccacc gcgattgaca tacatcggcg
    13441 tttcggtggt gtcttcttcg gaaattttcc cgcggttagt cgggcgaaca tatgagagtt
    13501 tgtgtttgat tttcacacgg ttctcatcaa acggttcgat ttccaggttg agcgagacct
    13561 tacctttggt tttcgtgttc atcacaccgg aagcgacttc actgagaact gcgccgattt
    13621 ttgtttcaaa tacgccgccg tccagctccc cgataaatgc ctgcacatca gtactgcgtt
    13681 cgctagccat tttgttgctc ctcatcatat cgaccctgca aggtcggttg gtttctccac
    13741 aaaacagaga agaacacctg cggtggcagc cgcccggatg gattgggtta tgagcccgtc
    13801 gtccggtgat gctcttctct gttttgtaaa aagagcggta ccagccggaa gcaagtgtac
    13861 aaactggtac cgccaaagca gtggctgttg tggtgaccgg tgctgatctc cggcttgcgg
    13921 ttatttcaga ctctcacggg cgtttaattg ccccgccgaa cagctctttt ccgcaatagc
    13981 tgcaatgtct ttcgcgcatc agcctgcgca ttcaccacaa cgctgagagc acttagccag
    14041 ttacggcacc acactttgtc gcggttccat aaatgccctc atcgttgcac cctggtctct
    14101 tcccaggtgt caaaccgaac cgccacgctg gttaggcgtc ttatcagcat catcattgac
    14161 ttgcacattc cggctacctg gtttgtttgc ccgagcaagg agtggattgt cccctttaac
    14221 gtccccagac cgctaacgac gcatgtgcca tacgccgtgt tacaaccaaa ttttgttagt
    14281 accttgtttg tatgtctgga aagaaagata aaatgaagtt gcgcattatg caagtgtttt
    14341 tattgcgaga tatgcaattt ggtgagtaat gaaaagccac cttctggtgg ctaattgata
    14401 ttgaggtagg gggttaattg tgtcgcttaa gggtttgtga ctgactgatt aagacctttc
    14461 caaagaccat aaaccgatgt tcgttttcgc tggtaattcc ccattcgcgg taaatctgat
    14521 tatcagaaat taccagcagt ttatcaggta tcatttgcag tcgtttgacg taaattttat
    14581 catcaaaacc aaatacatat ataccatccc catcaaactg attgatactg atatcaacga
    14641 agatgagatc tcctggctca atggttggac acatactgtc cccacgaacg ttgataactt
    14701 taatgtgatt tgctggtcgt ccaccaaaca tcgatacagc attatcagtt ctgtattcaa
    14761 tggcatgaat cacatcaatg acatcaccgc cctggataag gccatttccc gcactggcac
    14821 tgacatccag catttcaata cggaatacat ccttcacctg cgcaacatcc tcactaatac
    14881 tgtttttaca tacagtatta cttttgaggt ctgaggtaaa gagatcagca atatcaacac
    14941 ctaagctcct ggcaatatta ctcagggctt gttcagtgaa ttgtttctgc ttacctgttt
    15001 cgaggcgcga gatattcgcc gcatccactc ctattgcttc agcgagatcg gcgattttca
    15061 tgttcttcgc ctggcgaagt tgtctgactc gatttcctat gttcatgcgt ttattacatt
    15121 tctttattgc gcgttaagca aatcaacttg cgcaaaatat ttgcgtgaaa taatatgctc
    15181 atcacgcaat atgtggaggt tatatgcaat caccattacg gaatgtgcgt aaggcgcacg
    15241 gatttacttt gcagcatgtt gctgcgggcg ttcaggtcaa tccagcgacg ctgagtcgta
    15301 ttgaaagact ggaacaaatt ccatctatcg atcttgcaga acgtctggcc aattttttta
    15361 agggtgaaat cagcgaaatg cagattcttt atccggcacg ttttcaatct agccaaaacc
    15421 agaatgggtt taaaccacag gaacaggagg taagccgtgg gtaagcatca ctggaaagtg
    15481 gaaaaacaac ctgagtggta cgtgaaagct gtcagaaaaa ctatcgcggc attgcctggg
    15541 ggttacgctg aagctgctga ctggctggat gtaacagaga acgctttatt caaccgcctt
    15601 cgtgcagatg gcgatcagat tttcccgctg ggatgggcaa tggttttaca gcgtgcggct
    15661 ggcactcact acattgcgga tgctgtcgca cagtctgctg gtggggtgtt cgtatcgctt
    15721 cctgaaattg aggcagtaga gaacgccgat ataaaccagc gcctgctgga agtcattgaa
    15781 cagatcggca gttattccag acagattcgt tcagcaatcg aagacggtgt agtggaaccg
    15841 catgagaaga cagcaattaa cgacgaactg tatctttcaa tttcgaagct ccaggagcat
    15901 gcagcactgg tctacaaaat cttctgcgct ccagaaaata gtaacgcccg cgagtgtgca
    15961 gctccgggcg tcgtggcgtc gattgcttct ggttgtggag aaactaacgc atgaatagtt
    16021 taacggcaaa taaccgtttg tcgcaacagc tggtggtcag cgtcgctgaa cacctgttgt
    16081 tacggcatga atgcagatta ccaaatcacc tggctgtaag taaccacaga gaactttacc
    16141 tgactgtggg gggcgagttg tgcaggaact taaccgctgg tttcgtgacg gaagagggct
    16201 ttatgtccat gttattcgtt gggagccaga aacacagcgc gttatctatc ttcgcaaaga
    16261 ctacccgcat gagtgcttta gtcctttgtg gaaattcagg cgtgattttg ttgagtgtga
    16321 aggaccacca gcatattgat tctgccattc cgggacgtta cactgttcag gcaccttata
    16381 aagcgggtgc cgggattggc gtcctgaaat tgtcaacggc gatgtatgac gcgccagcgt
    16441 cttttttatc gtccgcattt gctcacatcc agattatggt gggctgggcg ggggcaccga
    16501 aaggtgcgcc ggtctccgtt gacgccggtt acgccaaccc cgtccagttc accaccagtg
    16561 aaattggcgt ttccggtggt ggaagttttt cactgtcaac ggaggctgcc atcatggcta
    16621 cgaacccagc cctcactcaa cctgaaatca ccattgacaa cggccaggcc gttaccactt
    16681 ctttggctgt tgccaactcc ttctccaagc gtcacgacga tgtgctgaaa aagatccgca
    16741 ctctggattg ttccccagag ttttgtgccc gcaattttgc ggagacatcg atttcggtaa
    16801 atcaaccgaa cggtggtaca cgcaagctcc cttgctatca aatcacccgc gacggttttg
    16861 cgtttcttgc tatgggtttc acgggtaaac gtgctgcccg gttcaaagag gcatacatca
    16921 atgcctttaa ccagatggaa aaactgcttt caaagccatc cacgctgagc gatgccgcag
    16981 ataacgccag cgtgctttac tcccacctgt cggtaatcca caaggtctgg ctgcagcagc
    17041 tttatcccat gttggcaaaa gccgaatccc cgctggctgt aagtctgtat gaccgcatca
    17101 acgacgcggc gctactggcc agtctcataa atttgtcgct gaacccttca gaggtaaggg
    17161 ggcgcaaatg atccggaata ttttcaaacg gtttaccaat cagactttcc gttgtcctcg
    17221 tcctggtcag tggtacacca cgcctgcagg gcatgttcta cgtgtcagcc tggttgaccg
    17281 tgaatgtcag aaggtgattt gtgaaccgct gggccgtaat taccgcatca gtatgccgct
    17341 tatagccttt tgctccggaa aaatgtttaa gcgtctggga ggtgtggcgt gaactgtttt
    17401 cagtttgtgt gcggatgtgc tttcgataac ccgattcagc gcctgattat gttgcgcgtt
    17461 ttgatgtcgg gttcttcaga cggtgaaggc gagagagtta ttgatcatca ggtgcttgct
    17521 gatttctgct gttgttctaa gcaagcgata ttcagggaaa ccctggcact ggaaagagct
    17581 ggttatcttc atatccgaaa aattgcaacg cttactattg atgcaaaagc cagactacaa
    17641 cctgcgcgtg gctacacaat tctcatgctg cggaaggagg ttgtatgagc cgttacgccc
    17701 ccacaccgga agttatggct attggtcaaa ttaatatttc cggcaatgtt acacctgcga
    17761 cctggtggaa atatattcga ctacccagtg ggcgtccgga tgcgacggct atcgctctgc
    17821 tttcagagat cgtttactgg taccgcccga cagaggtcag ggatgagcac accggagcgt
    17881 tgctgggata tcacaagcgt tttcagggcg acaaactgca aagaagctac caggcgtttg
    17941 ctgagcagtt tggtttcggg aaaagggaaa ccgcagatgc gctgaagcgt ctgcgcgatg
    18001 cagggtttat tactctggat ttacgcacgg tggaaatgct cgatggggtg aaatgcagca
    18061 atattttgtt tgtcgggatc aacccacagg caattgcggc catcaccaca ccttcttctg
    18121 tttcgccaga aagtaacagc aataatgcaa tcagcgatac agctattacg ttaaaacgga
    18181 acaccccccg acgtcataac ggaacagggg atacgccgaa tgttgataca aatacagaga
    18241 ttactacaga gattacaacg gagactaaaa acactattga tgcatccgct gacgcgtctg
    18301 cgccagcgcg ttctgcccga caggaatatt caccggaatt tgaacaggcc tggcaggaat
    18361 atcccaaacg tgctggtggc aattcaaaat cagcagcctt caaagcctgg aaagcccgtt
    18421 tgaacgaggg ggtaaacccc gaaaccatgc tggaaggtgt gaaacgctac gcgggctggg
    18481 tatctgcgat gggcaatagc ggcacacaat ttgtgaaaca ggctgtcacg ttctttggtc
    18541 cggatcgtca tttcgaagaa tcctgggaag ttcctgcggt atctgcagcc agacgcgagg
    18601 acccgtactt caaagccagt tacgacaacg tggactacag ccagatcccg acaggattca
    18661 gggggtgatc atgagtctga tgaacgatgt acagaaattc attgaagccc atccggggtg
    18721 tacttcaggt gacattgcgg atgcttttgc tggttactca cgacagtgcg ttctgcagtc
    18781 agcaagcaag ttacgtcaga gtggccgtgt ggctcactgt tgtgaaggga aaacacgtag
    18841 acatttccca caccaggctg agatatcgca ggaggagaaa ctgcaacctg ttcttgaaac
    18901 catacctgtg cgcaatttct atgtcggcac taacgatccc cgggtgattt tgtgcctgac
    18961 ccgccaggcg gaagaactgg agtccagggg cttataccgt cgtgctgcaa ccgtgtggat
    19021 ggcggcattc cgtgaaagcc actcccagcc agaacgaaac aattttctgg cgcgtcgtga
    19081 gcggtgctta cggaaaagca gaaagcgcgc tgtagcgggt gatgagtggt atctgtcagg
    19141 gaattacgtg ggggcttaat gagtaataaa tattgccagg cgctggtgga actacggaac
    19201 aaaccagccc atgaactgaa ggaagtgggc gatcagtggc gcacgccgga caacattttc
    19261 tggggaatta acaccctgtt tggcccgttt gttctggatc tgtttactga cggtgataac
    19321 gccaaatgtg ccgcgtatta cacggcggaa gacaacgcgc tggcacatga ctggtcagaa
    19381 cgtcttgcgg agcttaaagg tgctgccttt ggtaatcccc cgtacagccg cgccagtcag
    19441 catgagggac aatacatcac cggcatgcgt tacatcatga agcatgccag tgccatgcgt
    19501 gataaaggcg ggcgctatgt tttcctgatc aaagcggcca ccagcgaagt gtggtggccg
    19561 gaagatgcag atcatattgc ttttattcgc gggcgtattg gttttgaact gccagcctgg
    19621 tttatcccga aagacgaaaa gcaggtgcca acaggtgctt tcttcgctgg tgctattgct
    19681 gttttcgaca agacctggaa gggagcggca atcagctaca tcgggcgcga tgaacttgag
    19741 gcatgtggtg aggcctttct ggcgcaggtt cgccagcagg cggaaaaact ggtcagggag
    19801 atggcggcat gacgacatta actcaatgcc agcagcaggt gctggatatg ctgatttctt
    19861 atcagaaaga acgtggcttc ccgccaacca atcaggaggt ggcaaccatg ctgggatacc
    19921 gttcggtgaa tgcagcggtg gagcatcttc gagcactgga gaaaaaaggt gtcatcacga
    19981 taaagcgtgg cgtggcccgg ggtatcactc ttcataccgc ggtgaaggac gacgacagcg
    20041 aggcggtcgg gattatccgc gccctgcttg ccggtgaggc aaacgccagg ctgcgtgcag
    20101 cccactggtt acatgagagg ggcctgaaag tatgaagcta atactgcctt ttccgcccag
    20161 cgtgaacacg tactggcgac accctaacaa aggggcgttt gctggtaaga gcctgataag
    20221 cgcggcgggg cgaaaattcc agagcgcggc gtgtgcagca atagttgagc agttacgtcg
    20281 tctgccgaaa ccaacgtcgg cacctgcttc agtggagatc gtgttgtttc ctccggataa
    20341 ccggatccgc gatctggaca actataacaa ggcgctgttt gacgccctga cccacgcggg
    20401 tgtgtgggaa gacgacagcc aggtgaaaag aatgctggtg gagtggggac cggttatccc
    20461 ggaagggaag gtcgagatca ctatcagtaa gtacgaaaaa gcgagttgca aattagcaac
    20521 tcggtaacgg aattgagcaa caccctaaat ttgggtatta cctcgttaaa gatactgtat
    20581 ttatgaacag tgtatccttg ataactatta aaaatcgcag taagttcatc ctgcatcaac
    20641 gaaaagggag tgcagtcccg ctcgtggata aaaatttgtg gagaaaccaa tgaatcagtt
    20701 gcttgtaatt gatggcgttt ctgtgcgcca gtacttcgaa tctaactact gtcttaacga
    20761 ccttcagaaa gctgctcttc ttgccgctgg tgagaatcgc tcctcccgtt cgctggaagc
    20821 tcacgagttt atgcgtcgtc ctgaaacgaa ggctcttgtg gaattattgg aagaagaaac
    20881 tacgggagat tcccgtagta ttcctgtcat caccattcag gggcgcaatg gtgggacgta
    20941 tgtctgtaaa gagctggtct atgcatatgc aatgtggatc agcccggcat tcagcttaaa
    21001 agtgatacgt acttttgatg cgcttcataa ttcatcacca gaagaaacca catccgacaa
    21061 aattaaatcc ggggtcattc tgcttgaatc agcagcaaag actctaaatc tgtcaaactc
    21121 ctcgaaactt ggtgcatacc agaaattatc aaaggtagct ggtcttcctg aacttatgcc
    21181 gatctatgcc attgatgcac ctgctgatgc gccagatggt tcaagccgcc ctacgctgtc
    21241 gctgagtgca ctgctgaagc agtatggtat ccgcctgacg gctaatcagg catatcacca
    21301 gatggtgaaa ctggggatcg tcgagcagcg cgaacgatac agccgtaccg cgattaacaa
    21361 catcaaaaaa ttctggtcgc tgacagcgaa aggctgcatg ttcggcaaga acatcaccag
    21421 tcctgcaaat ccgcgcgaga cgcagccgca tttcttcgaa tcccgattcc ctgagctgtt
    21481 aaagctgctc gataccgttc actgatggga ggcgtggagc atgagaatta caccacccca
    21541 tctgcagcca gttttatcca gggttaaacg ttttgttgaa cgaatgccgg aaggcgcaac
    21601 actgacccag atatcacaga aagtgcaggc gtacagtcta ctgaataaaa gggataagga
    21661 gatactcatt ggcattatcc gcgacagtgg acttctggtc gttgcgaatg atggaagaac
    21721 tacaacgtta catcatccta aatttggaca tcaggcagta aatagtgaaa taccagtaaa
    21781 aacagaggaa cccgttgtga ttaaaaagac cgttactccg gatgaattac gcaggcatgc
    21841 tgaggagctg atcagggctg cggaagaagc agagaagaaa tttaatgatc gtgcggaaat
    21901 taaaaagcaa ctggatcctc tgaaactgga aatcctccag gcgtatggaa tggcaagtcg
    21961 taaatttgat gagtttgttg atgctatggc ggatatgggg aaagcggtac agaaactgaa
    22021 acagattgtg ctgtgaggtt ctacgttgag agcactactg acccctgaaa ttgccccgcg
    22081 tatggggatc gtattgttca ggccaggttc agagctgatg cccctgttta tgcaggggcg
    22141 tgtcctgctg gagcctgagc cggagcgtta ttcatctttc gccagtggtg ccgttccggc
    22201 ggcatcacaa ccgctggcgg atgatcctgc cgttcgggcc gtgttccgca atgaggcggt
    22261 gatccgtcgt gctggtggcg tggaatgcct tgagagctgg ttacttcgtg aaaagggctg
    22321 tcagtggcct cattccgact ggcacagcga gaacatgacc acaatgcggc acgcgccagg
    22381 cgcaatccgt ctgtgctggc actgtgacaa tctcctccgt gaccagttca cggaacggct
    22441 ggaatcaatg gcaacggata actgtgcccg ctgggtgttg tctgttgtgc gccgtgatct
    22501 tggttttgat gacagtcacg ttgtgacaat gccggaactg tgctggtggc tggttcgtaa
    22561 tgacctggcg gatgccttac cggaaagtgc agcccgtaag gtactgagat taccgaagac
    22621 ggttgtgccg tctgtcaccc gggaaagtga ccttgtgcct tcggttcccg ccaccagtat
    22681 tatccgggat aaagcgaaaa aggtgctggc gctgaaagtg gatccggagt cgccggagtc
    22741 ttttatgtta cgcccaaaac gtcgccgctg ggttaatgaa aagtacacgc gctgggttaa
    22801 gacgcagccg tgcgcatgtt gtggaaaacc tgctgatgat ccccaccacc tgataggtca
    22861 cggtcagggc ggaatgggta caaaagcgca tgacctcttt gtgttgcctt tgtgcagaaa
    22921 gcatcacgac gagctgcatg cggataccgt ggcatttgaa gagaagtatg gctcccagct
    22981 ggagctgata tttcgtttta tcgatcgcgc gctggcgatt ggtgtgctgt cctgattttg
    23041 tggagaaagt tgatgcgtga tattcagatg gttctcgaac gctggggggc atgggcggca
    23101 agtggtaacg ccggggtgga ctattctccg atagctgctg gatttaaagg ccttttacca
    23161 tccaccgcta aacctcgccc ggcctgcagc gatgatgacg gccttatcat cgaaaactgc
    23221 cttacgcgcc tgaagaagaa aaaaccggac gagtattcgc tgctggtagc tcattatctg
    23281 ctgcgcatat caaaaaggca gattgccaga acaagaaaga agagcgaaaa ggcaatacga
    23341 attgagatgc agattgctga aggatttatt gacggatgtc tgtcgatgct gggtgtaagg
    23401 ctggagatgg acgactggct gcccaaaaaa gtaaaaaatg attagcgcgg tccgcaaaaa
    23461 gtatgtcagt atgttaagag tggttactac gccacacaac ttaaacccgc cgcctggcgg
    23521 gttttttatg actgaaatcg catcagtaca gtaaacgtgc tggtggtgaa tacctgtctt
    23581 tcagcttgct ggctttttag acaagagtta ttggtatgtc acgttaaccg gaaaagggaa
    23641 aaagacatgc t
//
LOCUS       DAZGVQ010000075        23479 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000075 DAZGVQ010000000
VERSION     DAZGVQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.075"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(383..1180)
                     /gene="ybcM"
                     /locus_tag="ACHLAG_002823"
     CDS             complement(383..1180)
                     /gene="ybcM"
                     /locus_tag="ACHLAG_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0656015.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1190..1741)
                     /gene="ybcL"
                     /locus_tag="ACHLAG_002824"
     CDS             complement(1190..1741)
                     /gene="ybcL"
                     /locus_tag="ACHLAG_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFP0656016.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(1910..2242)
                     /gene="emrE"
                     /locus_tag="ACHLAG_002825"
     CDS             complement(1910..2242)
                     /gene="emrE"
                     /locus_tag="ACHLAG_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFP0656017.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2576..2890)
                     /gene="fliE"
                     /locus_tag="ACHLAG_002826"
     CDS             complement(2576..2890)
                     /gene="fliE"
                     /locus_tag="ACHLAG_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFP0656018.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            3105..4763
                     /gene="fliF"
                     /locus_tag="ACHLAG_002827"
     CDS             3105..4763
                     /gene="fliF"
                     /locus_tag="ACHLAG_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFP0656019.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIATIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4756..5751
                     /gene="fliG"
                     /locus_tag="ACHLAG_002828"
     CDS             4756..5751
                     /gene="fliG"
                     /locus_tag="ACHLAG_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFP0656020.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5744..6430
                     /gene="fliH"
                     /locus_tag="ACHLAG_002829"
     CDS             5744..6430
                     /gene="fliH"
                     /locus_tag="ACHLAG_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFP0656021.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            6430..7803
                     /gene="fliI"
                     /locus_tag="ACHLAG_002830"
     CDS             6430..7803
                     /gene="fliI"
                     /locus_tag="ACHLAG_002830"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFP0656022.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSDKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7822..8265
                     /gene="fliJ"
                     /locus_tag="ACHLAG_002831"
     CDS             7822..8265
                     /gene="fliJ"
                     /locus_tag="ACHLAG_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFP0656023.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLDKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            8262..9389
                     /gene="fliK"
                     /locus_tag="ACHLAG_002832"
     CDS             8262..9389
                     /gene="fliK"
                     /locus_tag="ACHLAG_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFP0656024.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSIQGRVTGNSGVDIFA"
     gene            9494..9958
                     /gene="fliL"
                     /locus_tag="ACHLAG_002833"
     CDS             9494..9958
                     /gene="fliL"
                     /locus_tag="ACHLAG_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFP0656025.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGVTLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            9963..10967
                     /gene="fliM"
                     /locus_tag="ACHLAG_002834"
     CDS             9963..10967
                     /gene="fliM"
                     /locus_tag="ACHLAG_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFP0656026.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTER
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10964..11377
                     /gene="fliN"
                     /locus_tag="ACHLAG_002835"
     CDS             10964..11377
                     /gene="fliN"
                     /locus_tag="ACHLAG_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFP0656027.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            11380..11745
                     /gene="fliO"
                     /locus_tag="ACHLAG_002836"
     CDS             11380..11745
                     /gene="fliO"
                     /locus_tag="ACHLAG_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFP0656028.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            11745..12482
                     /gene="fliP"
                     /locus_tag="ACHLAG_002837"
     CDS             11745..12482
                     /gene="fliP"
                     /locus_tag="ACHLAG_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFP0656029.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            12492..12761
                     /gene="fliQ"
                     /locus_tag="ACHLAG_002838"
     CDS             12492..12761
                     /gene="fliQ"
                     /locus_tag="ACHLAG_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFP0656030.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12770..13555
                     /gene="fliR"
                     /locus_tag="ACHLAG_002839"
     CDS             12770..13555
                     /gene="fliR"
                     /locus_tag="ACHLAG_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFP0656031.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            13845..14468
                     /gene="rcsA"
                     /locus_tag="ACHLAG_002840"
     CDS             13845..14468
                     /gene="rcsA"
                     /locus_tag="ACHLAG_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFP0656032.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(14512..14700)
                     /gene="dsrB"
                     /locus_tag="ACHLAG_002841"
     CDS             complement(14512..14700)
                     /gene="dsrB"
                     /locus_tag="ACHLAG_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFP0656033.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14863..15090
                     /gene="yodD"
                     /locus_tag="ACHLAG_002842"
     CDS             14863..15090
                     /gene="yodD"
                     /locus_tag="ACHLAG_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFP0656034.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            15388..16203
                     /gene="yedP"
                     /locus_tag="ACHLAG_002843"
     CDS             15388..16203
                     /gene="yedP"
                     /locus_tag="ACHLAG_002843"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFP0656035.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWGEGLDHFFSAR"
     gene            complement(16200..17894)
                     /gene="dgcQ"
                     /locus_tag="ACHLAG_002844"
     CDS             complement(16200..17894)
                     /gene="dgcQ"
                     /locus_tag="ACHLAG_002844"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFP0656036.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRDAV
                     TEFEQHRDEHAWKIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLYQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(18132..18314)
                     /gene="yodC"
                     /locus_tag="ACHLAG_002845"
     CDS             complement(18132..18314)
                     /gene="yodC"
                     /locus_tag="ACHLAG_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFP0656037.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRPAEEV"
     gene            complement(18393..19310)
                     /gene="yedI"
                     /locus_tag="ACHLAG_002846"
     CDS             complement(18393..19310)
                     /gene="yedI"
                     /locus_tag="ACHLAG_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFP0656038.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKVKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            19483..20403
                     /gene="yedA"
                     /locus_tag="ACHLAG_002847"
     CDS             19483..20403
                     /gene="yedA"
                     /locus_tag="ACHLAG_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFP0656039.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(20392..20862)
                     /gene="vsr"
                     /locus_tag="ACHLAG_002848"
     CDS             complement(20392..20862)
                     /gene="vsr"
                     /locus_tag="ACHLAG_002848"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFP0656040.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(20843..22261)
                     /gene="dcm"
                     /locus_tag="ACHLAG_002849"
     CDS             complement(20843..22261)
                     /gene="dcm"
                     /locus_tag="ACHLAG_002849"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFP0656041.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     QNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(22328..23023)
                     /gene="yedJ"
                     /locus_tag="ACHLAG_002850"
     CDS             complement(22328..23023)
                     /gene="yedJ"
                     /locus_tag="ACHLAG_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFP0656042.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSSAG"
     gene            complement(23063..23344)
                     /gene="drpB"
                     /locus_tag="ACHLAG_002851"
     CDS             complement(23063..23344)
                     /gene="drpB"
                     /locus_tag="ACHLAG_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFP0656043.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         5801 a         6121 c         5896 g         5661 t
ORIGIN      
        1 aatttctgct gtagttgcat cttaacgtat aaaccagatt taataacaac gaactagatt
       61 taataacaac aaactagatt taataacaac gatatccgta cctatccgga tatctaatgt
      121 cataagcatc cctttaatta tcccctcaac ctcttgtaac tttcccactc ttgaactcca
      181 catagtccaa acgccggatg caaaaaaatc acacgtgcat ttctgaaatt gtgatggagg
      241 acgatagtat caattatatg tgcttataaa cgtccccgat tagcgcagaa gtcaccggat
      301 tgttcaggct ccaataggtt gagtatgccg tatttatttt ccaaaataaa aatcgatgct
      361 gttgctcctt cataaaatac ttttactctc tggacgcttt tcttagctct cttggtgtta
      421 tcttaaaacg attatggaac ctttcagtaa aacgagaagg acacttgtaa ccattttccc
      481 tggcaatctc gctgatgggt tttactgtcg tttgtatagc agacaacgca ttatttaacc
      541 tcacgtcgtc caatatactt tgaaaactta ctccctcgct tgccagacgg cgatgcaatg
      601 tggaaacaga aatgtagaga tatctcgcta ccttgtttgc tgtccatttt gttccaggtt
      661 cggatagcat taggttataa caacgactta ttaatgattg tttactatct gataaaagta
      721 aacaattaac atgatgtgct cctaatgaaa gtagaacgcc cattgctaag tgttcctgaa
      781 tttgcgttga gaagcctcgg gaaatagatg tttttagttg ctcccaacaa tatattaact
      841 cgggattctg aggtaagcag aaacctgtcg tgttacgaat ttgatcggtt atcgcataaa
      901 gattttggaa cttttcaatt agatcaatgg gataataaag catttctgca agataaagac
      961 ctgcttctgg gtaattcgca atataaaatt catatccaca gggaaataat attatctgat
     1021 tattatcaac agttagacta tgcgtctccc aattgataac tttttttccc tgacggatac
     1081 gacataaagc tggcataaga ggcttaaccc tgtgaatctc atgatgttta tgcatccgta
     1141 tttcttcaat ctttaggtta gtcctatctt ttgccagcat actcttatcc tactttacct
     1201 cataaacggg tattatctca gcggttgcaa ttttattggc attaagcata taaccaacta
     1261 acgctccgct ggaattagaa tccacaggaa tcttatcagt ttttagagcc catattttaa
     1321 actggtaatg atggggttta tctcctttgg gaggacatgc tccaccaaat cccgcatagc
     1381 caaaatcatt tcggccttgg acagcaccag tcggaagttt tgtaccatca cgtcttcctg
     1441 catcagcggg caaatatgtt accgttgctg gaatattagc aacagtccag tgccaccatc
     1501 cgctgcctgt aggcgcatct ggatcataca cagttacggc aaagcttttg gtgccttctg
     1561 gagcaccaga ccaggttaat gagggcgatg tattaccccc ctcacaccca aatccagaaa
     1621 atacatgaga cgttgtaagt tgctctcctg tttttatttc attactagtg acctgaaatg
     1681 ctgcggcctg cgcagaaaat gttatgaatg ccaatacact tgaaacgata agttttttca
     1741 taaaaacctc tttgttgtga cctatcgtta ttttatttga tgtgcattga tctcattatg
     1801 caaaaaggcg caatgatcgc gcaaatgcaa tcataattat aaagttgccc cgaacattag
     1861 ccataatatg cacaaatcag attcctgtta tctcaaggat gattattttt taatgcggtg
     1921 cgcttcgtga caacaagtta atgactaaca caccggcaca aatcaacatc atgcctatga
     1981 tggctggcaa gtccagtcgt tggccaaaaa atccccagga aagcaaacta atcagaacga
     2041 taccgactcc tgaccagata gcataagcaa tccctgtagg tatataagcc agtgtctgag
     2101 ctaataacca aaatgatgca caatagcaaa taattgtgcc aacagagggc cataaccgtg
     2161 taaacccctc tgaaaacttc attaaggttg taccaatgac ctctgcaagt attgcgccac
     2221 caagataaat ataagggttc atagcatatt ctttcctgtt catactggag agaattgtac
     2281 tacagtttga acacaactca ccgttttcat cctggtgtat gtgtatccag ggggctctgg
     2341 cgtggcaaga gataaaggca ggaacaataa tgatcagaag gaaccccagg ctaacaaaag
     2401 agttcactct tcctatgagc aaagcgaaga caaaacatgt tatcgcgtat acggatcgtg
     2461 gatggcagaa aactttcagg atcaggtatc cattttaaac cagaaatagg gtgagtttgc
     2521 cccatccatg ccccacgcgg gcagatggga tgtaattaac aacaggttac aaaacctaca
     2581 cttgcatgga catcacttcc tgatacgccg ccaccagctt attacgcacc tgaatcccca
     2641 tttgcataga aactgaggct ttttgcatat cggtcatcac atcgtttaac gccacgccgg
     2701 gttcaccgag ggtgaatttt tctgcctgcg tgcgggcagc tgtttgtgta tcgctaatgc
     2761 gatcgagcgc ggcgtgcagc tgcccggcaa aactaatggt cggttgcggc agtgaatcct
     2821 gcgcacgcgc actcatcgcc gtagcctgta actggcttat aaccccttca atcccctgta
     2881 tcgctgacat tttcgtctcc tggataattt ctggtagcaa agcctaccag taagtcaata
     2941 agacaaaggc gctaaatagc aacaaaaaaa cgggtttatt ggcggataga aaaaaacgaa
     3001 agcacaaata atggcagcgt caatttttcg agtttgctga cccgggagtg agtcttgttc
     3061 cactttgcca ataacgccgt ccataatcag ccacgaggtg cgcgatgaat gcgactgcag
     3121 cccagacaaa atctcttgag tggcttaatc gcctgcgtgc gaatccgaaa attccattga
     3181 ttgttgccgg ttccgcggca gtggcggtca tggtcgcact gatcctgtgg gcgaaagccc
     3241 ccgactaccg cacattattc agcaatcttt ccgatcagga tggtggcgca attgtcagcc
     3301 aactgacgca aatgaatatt ccttaccgct tcagcgaagc cagcggcgct attgaagttc
     3361 cggcagataa agttcacgaa ctgcgtctgc gcctggcaca acaaggtttg cccaaaggcg
     3421 gcgcggtcgg tttcgaacta ctcgatcagg aaaagtttgg tatcagccag ttcagcgaac
     3481 aggtgaatta tcagcgggcg ctggaaggcg agctttctcg taccatcgca actatcggcc
     3541 cggtaaaagg ggcgcgcgta catctggcaa tgccgaaacc gtctttattc gtccgtgaac
     3601 aaaaatcccc ttctgcatcg gtgacggtaa atctgttacc cggccgcgca ctcgatgaag
     3661 ggcaaattag cgccattgtg catctggttt ccagcgccgt tgctggtctg ccgccgggaa
     3721 acgtcacgct ggtggatcag ggcggacatc tgttaaccca gtccaatacc agcgggcgcg
     3781 atcttaatga tgctcagttg aaatatgcca gcgatgtcga aggccgtatt cagcggcgta
     3841 ttgaagcaat cctgtcgcct attgttggta acggtaatat tcacgcccag gtcacggcgc
     3901 agctggactt cgccagtaaa gaacaaacgg aagaacagta tcgccctaac ggtgatgaat
     3961 ctcatgcggc gcttcgttca cgccagctta atgagagcga gcaaagcggt tccggttatc
     4021 cgggcggcgt accgggggcg ttgtcgaatc aaccggcacc tgcgaataac gcgccaatca
     4081 gcacgcctcc ggcaaatcaa aataaccgcc agcagcaggc gagcaccacc agcaatagcg
     4141 ggccgcgtag cacacagcgg aatgaaacca gtaactacga agtcgatcgc accattcgtc
     4201 ataccaaaat gaacgtgggc gatgtgcaac gtctgtcagt cgcggtagtg gtgaattaca
     4261 aaaccttgcc agacggtaaa ccattgcctc tcagcaacga acagatgaag caaattgaag
     4321 atctgacccg cgaggcgatg ggcttttctg aaaaacgcgg tgactcgctc aatgtcgtta
     4381 actcgccgtt caatagcagt gacgaaagcg gcggagaact gccgttctgg caacagcaag
     4441 cgtttatcga tcagttgctg gctgccggtc gctggttgct ggtactgctg gtggcgtggc
     4501 tgctgtggcg gaaagcggta cgcccgcagt taacacgtcg cgccgaggcg atgaaagctg
     4561 tacagcaaca ggcgcaggcc cgtgaggaag tggaagatgc ggtggaagtc cgcctgagca
     4621 aagacgaaca actccaacaa cgacgcgcta accaacgtct gggggcagaa gtcatgagcc
     4681 agcgtatccg tgaaatgtct gataacgatc cgcgcgtggt ggcgctggtc attcgccagt
     4741 ggataaataa cgatcatgag taacctgaca ggcaccgata aaagcgtcat cctgctgatg
     4801 accattggcg aagaccgggc ggcagaggtg ttcaagcacc tctcccagcg cgaagtgcaa
     4861 accctgagcg ctgcaatggc gaacgtcacg cagatctcca acaagcagct aaccgatgtg
     4921 ctggcggagt ttgagcaaga agctgaacag tttgccgcac tgaatatcaa cgccaacgat
     4981 tatctgcgtt cggtattggt caaagctctg ggtgaggaac gtgccgccag cctgctggaa
     5041 gatattctcg aaactcgcga taccgccagc ggtattgaaa cgctcaactt tatggagccg
     5101 cagagtgccg ccgatctgat tcgcgatgag catccgcaaa ttatcgccac cattctggtg
     5161 catctgaagc gcgcccaggc cgccgatatt ctggcgttgt tcgatgaacg tctgcgccac
     5221 gacgtgatgt tgcgtatcgc cacctttggc ggcgtgcagc cagccgcgct ggcggaactg
     5281 accgaagtac tgaatggctt gctcgacggt cagaatctca agcgcagcaa aatgggcggc
     5341 gtgagaacgg cagccgaaat tatcaacctg atgaaaactc agcaggaaga agccgttatt
     5401 accgccgtgc gcgaattcga cggcgagctg gcgcaaaaaa ttatcgacga gatgttcctg
     5461 ttcgaaaatc tggtggatgt cgacgatcgc agcattcagc gtctgttgca ggaagtggat
     5521 tccgaatcgc tgttgatcgc gctgaaaggg gccgagcagc cactgcgcga gaaattcctg
     5581 cgcaatatgt cgcagcgtgc cgccgatatt ctgcgcgacg atctcgccaa ccgtggtccg
     5641 gtgcgtctgt cgcaggtgga aaacgaacag aaagcgattc tgctgattgt gcgccgcctt
     5701 gccgaaactg gcgagatggt aattggcagc ggcgaggata cctatgtctg ataatctgcc
     5761 gtggaaaacc tggacgccgg acgatctcgc gccaccacag gcagagtttg tgcccatggt
     5821 cgagccggaa gaaaccatca ttgaagaggc cgaacccagc cttgagcagc aactggcgca
     5881 actgcaaatg caggcccatg agcaaggtta tcaggcgggg attgccgaag gtcgccagca
     5941 aggtcatgag cagggctatc aggaaggact ggcccggggg ctggagcaag gtctggcaga
     6001 ggcgaagtct caacaagcgc caattcatgc ccggatgcag caactggtca gcgaatttca
     6061 aactaccctt gatgcgcttg atagtgtgat tgcttcgcgc ctgatgcaga tggcgctgga
     6121 ggcggcacgt caggtcatcg gtcagacgcc aacagtggat aactcggcac tgatcaaaca
     6181 gatccaacag ttgttgcagc aagaaccgtt attcagcggt aaaccacagc tgcgcgtgca
     6241 cccggatgat ctgcaacgtg tggatgatat gctcggcgca accttaagtt tgcatggctg
     6301 gcgcttgcgg ggcgatccca cactccatcc tggcggctgt aaagtctccg ccgatgaagg
     6361 cgatctcgac gccagtgtcg ccactcgctg gcaagaactc tgccgtctgg cagcaccagg
     6421 agtggtgtaa tgaccacgcg cctgactcgc tggctaacca cgctggataa ctttgaagcc
     6481 aaaatggcgc agttgcctgc ggtacgtcgc tatgggcgat taacccgcgc taccgggctg
     6541 gtgctggaag ccaccggatt acaattgccg ctcggcgcaa cctgtgtcat tgaacgccag
     6601 aacggcagcg aaacgcacga agtagaaagc gaagtcgttg gctttaacgg tcaacggctg
     6661 tttttaatgc cgctggagga agtcgaaggt gtcctgcccg gcgcgcgggt ttatgccaaa
     6721 aacatttcgg ctgaaggact gcaaagcggc aagcagttgc cgctcggtcc ggcgttatta
     6781 ggtcgcgttc tggacggcag cgataagccg ctcgatggcc tgccctcccc cgatacgacg
     6841 gaaaccggtg cgctgattac cccgccattt aacccgttgc aacgtacacc gattgaacat
     6901 gtgctggaca ccggcgtgcg cccaatcaat gccctgctta ccgttgggcg tgggcagcgt
     6961 atgggactgt ttgccgggtc cggcgttggt aaaagtgtgc tgctggggat gatggcacgt
     7021 tacacccgcg ccgatgtcat tgtcgtgggt ttgattggtg aacgcggacg cgaagtaaaa
     7081 gattttattg agaatatcct cggtgccgaa gggcgtgcac gctccgtggt gattgccgct
     7141 ccggcggatg tttctcctct cctgcgaatg cagggtgccg cctatgccac gcgcattgcc
     7201 gaagattttc gcgatcgtgg tcagcatgtg ttgctgatta tggactccct cacccgctac
     7261 gcgatggccc agcgtgaaat tgcgctggcg attggcgaac cacccgccac taaaggttat
     7321 cccccgtcgg tgtttgccaa attaccggca ctggtcgagc gtgccggaaa tggcattagc
     7381 ggcggcggat cgattactgc attttatacc gtgctcactg aaggcgatga ccagcaggac
     7441 cccattgccg actctgcgcg ggcaatcctc gacggtcaca ttgtgctgtc tcgccgactg
     7501 gcggaagccg ggcactatcc ggctatcgat attgaagcgt cgatcagccg cgcaatgacg
     7561 gcgttgatca gtgagcaaca ttacgcgcga gtgcgcacct tcaaacagct gttgtcgagt
     7621 tttcagcgta accgcgatct ggttagcgtc ggcgcgtatg ccaaaggcag cgatccgatg
     7681 ctcgacaaag ccatcgccct gtggccgcag ctggagggct atttgcaaca aggcattttt
     7741 gaacgcgcgg actgggaagc gtctctccag gggctggagc gtattttccc gacagtgtca
     7801 taacccagga gataacggca gatggcagaa catggcgcgc tggcgaccct gaaagatctg
     7861 gcagaaaaag aggtagagga tgccgcgcgc ctgctgggtg aaatgcgtcg cgggtgtcag
     7921 caggcggaag aacagctcaa aatgctgatc gattatcaga atgaatatcg caataacctc
     7981 aacagcgata tgagtgccgg gatgaccagc aaccgctgga tcaactatca gcagtttatc
     8041 cagacgctgg ataaagccat tactcagcat cgccagcaac ttaatcagtg gacgcagaaa
     8101 gttgacattg ccctgaacag ctggcgagaa aagaaacaac gtttgcaggc ctggcagaca
     8161 ctgcaggaac ggcaatccac ggcggcactg cttgcagaaa accgcctcga tcagaaaaag
     8221 atggatgagt tcgcccagcg cgccgccatg aggaaacctg aatgattcgc ttagcgccct
     8281 taattaccgc cgacgttgac accaccacat tgcctggcgg caaagccagc gatgctgcac
     8341 aagattttct cgcgttgttg agcgaagcat tagcaggcga gacaactacc gacaaagcgg
     8401 ccccccagtt gctggtggca acagataagc ccacgacaaa aggcgagctg ctgatcagcg
     8461 atattgtttc cgacgcgcaa caagcggatt tactgatccc tgtggatgaa acactgcctg
     8521 tcatcaacgt cgaacaatcc acatcaacac cgttaaccac cgctcagacg atgacgttgg
     8581 ctgcggtggc tgacaaaaat acgacaaaag acgaaaaagc ggatgatctg aatgaagacg
     8641 tcaccgcaag cctgagcgcc ctttttgcga tgttgccggg ttttgacaat acgcccaaag
     8701 tgactgatgt gccgtcaacc gtgttaccgg cagagaaacc aacgctcttc acaaaactga
     8761 cttctgcgca actcacaaca gcacagcctg atgatgcccc cggcacgcca gctcagccat
     8821 taacaccgct ggtagcagaa gcccagagta aagcggaagt catcagcaca ccgtcaccgg
     8881 tgaccgctgc cgccagcccg ctaattactc cacaccagac acagccactg cccaccgtcg
     8941 ccgcgcctgt tttgagtgca ccgctgggtt ctcacgaatg gcaacaatca ttaagccagc
     9001 atatttcgct attcacccgc caggggcaac aaagtgcaga gttgcgtctg cacccgcagg
     9061 atttaggtga agtgcaaatc tccctcaaag tggatgataa ccaggcacaa atccagatgg
     9121 tttcaccgca tcagcatgta cgcgccgcgc tggaagcagc gcttccggta ctgcgtacgc
     9181 agctggccga aagtggcatt cagttagggc aaagcaacat cagtggcgaa agctttagtg
     9241 gtcagcagca ggccgcttcc caacaacagc aaagccaacg cacagtaaac catgaacctc
     9301 tggcggggga agacgacgat acgcttccgg ttcccgtctc tatacaaggg cgcgtaacag
     9361 gcaacagcgg cgttgatatt ttcgcctaac gtcagaggta gcaccgtaat ccgcgtcttt
     9421 tccccgcttt gttgcgctca agacgcagga taattagccg ataagcagta gcgacacagg
     9481 aagaccgcaa cacatgactg attacgcgat aagcaagaaa agcaagcgat cgctttggat
     9541 cccgattctg gtattcatta ccctcgcggc ctgtgccagc gcaggttaca gctactggca
     9601 ttcgcatcag gttgccgctg acgacaaagc gcagcaacgc gtcgtaccct ccccggtctt
     9661 ctacgcgctg gataccttca cggtcaattt gggcgatgcg gatcgcgtgc tttatatcgg
     9721 cgtaaccctg cgcctgaaag atgaagctac ccgctcgcgg ctgagtgagt atttgccgga
     9781 agtccgtagc cgtttgctgt tactgttttc gcgtcaggat gctgccgtac tggcgacaga
     9841 agaaggcaag aaaaacctga ttgccgagat taaaaccacg ctttccaccc cgcttgttgc
     9901 cgggcaaccg aaacaggatg tcaccgacgt gctgtatacc gcttttattc tgcgataacg
     9961 acatgggcga tagtattctt tctcaagctg aaattgatgc gctgttgaat ggtgacagcg
    10021 aagtcaaaga cgaaccgaca gccagtgtta gcggcgaaag tgacattcgt ccgtacgatc
    10081 cgaataccga acgacgggtt gtgcgcgaac gtttgcaggc gctggaaatc attaatgagc
    10141 gctttgcccg ccattttcgt atggggctgt tcaacctgct gcgtcgtagc ccggatataa
    10201 ccgtcggggc catccgcatt cagccgtacc atgaatttgc ccgcaacctg ccggtgccga
    10261 ccaacctgaa ccttatccat ctgaaaccgc tgcgcggcac tgggctggtg gtgttttcac
    10321 cgagtctggt gtttatcgcc gtggataacc tgtttggcgg cgatggacgc ttcccgacca
    10381 aagtggaagg tcgcgagttt acccataccg aacagcgcgt catcaaccgc atgttaaagc
    10441 tggcgcttga aggctatagc gacgcctgga aggcgattaa tccgctggaa gttgagtacg
    10501 tgcgttcgga aatgcaggtg aaatttacca atatcaccac ctcgccgaac gacattgtgg
    10561 ttaacacgcc gttccatgtg gagattggca acctgaccgg cgaatttaat atctgtctgc
    10621 cattcagcat gattgagccg ctacgggaat tgttggttaa cccgccgctg gaaaactcgc
    10681 gtaatgaaga tcagaactgg cgcgataacc tggtgcgcca ggtgcagcat tcacagctgg
    10741 agttggtcgc caactttgcc gatatctcgc tacgcctgtc gcagatttta aaactgaaac
    10801 caggcgacgt cctgccgata gaaaaacccg atcgcatcat cgctcatgtt gacggcgttc
    10861 cggtactgac cagtcagtac ggcaccctca acggtcagta tgcgttacgg atagaacatt
    10921 tgattaaccc gattttaaat tctctgaacg aggaacagcc caaatgagtg acatgaataa
    10981 tccggccgat gacaacaacg gcgcaatgga cgatctgtgg gctgaagcgt tgagcgaaca
    11041 aaaatcaacc agcagcaaaa gcgctgccga cgcggtgttc cagcaatttg gcggtggtga
    11101 tgtcagcgga acgttgcagg atatcgacct gataatggat attccggtca agctgaccgt
    11161 cgagctgggc cgtacgcgga tgaccatcaa agagctgttg cgtctgacgc aagggtccgt
    11221 cgtggcgctg gacggtctgg cgggcgaacc actggatatt ctgatcaacg gttatttaat
    11281 cgcccagggc gaagtggtgg tcgttgccga taaatatggc gtgcggatca ccgatatcat
    11341 tactccgtct gagcgaatgc gtcgcctgag ccgttagtga tgaataacca cgctactgtg
    11401 caatcttccg cgccggtttc tgctgcgcca ctgctgcagg tcagcggcgc actcatcgcc
    11461 attattgccc tgatcctcgc tgctgcctgg ctggtaaaac ggttgggatt tgccccgaaa
    11521 cgcactggcg ttaacggtct gaaaattagc gccagtgctt cgctgggcgc gcgtgaacgg
    11581 gttgtggtgg tcgatgtgga agatgcacgg ctggtgctcg gcgttaccgc agggcaaatc
    11641 aatctgctgc ataaacttcc cccttcagca ccaacagaag agataccgca gaccgatttt
    11701 cagtcggtca tgaaaaattt gcttaagcgt agcgggagat cctgatgcgt cgtttgttgt
    11761 ctgtcgcacc tgtccttctc tggctggtta cgcccctcgc cttcgcgcaa ctgccgggta
    11821 tcaccagcca gccgctgcct ggcggtggac aaagctggtc gctcccggtg cagacgctgg
    11881 tgttcatcac ctcgttgacg tttattccgg caattttact gatgatgacc agtttcaccc
    11941 gcatcatcat tgtttttggt ttattgcgta acgcgctggg aacgccctcc gcgccaccta
    12001 accaggtatt gctggggctg gcactatttt tgaccttttt tattatgtca ccggtgatcg
    12061 acaaaattta tgtcgatgcg taccagccat tcagcgaaga gaaaatatca atgcaggagg
    12121 cgctggaaaa aggggcgcag ccgctacgtg agtttatgct gcgtcagacc cgtgaggcag
    12181 atttagggtt gtttgccaga ctagcgaata ccggcccgtt gcagggacct gaagccgtgc
    12241 cgatgcgcat tttgctcccg gcctacgtga ccagcgagtt gaaaaccgca tttcagatag
    12301 gcttcacgat tttcatccct tttttgatta tcgacctggt gattgccagc gtgttgatgg
    12361 cattggggat gatgatggtc ccccccgcca ccattgctct gccctttaaa ctgatgctgt
    12421 ttgtactggt ggatggctgg caattgctgg tcggttcgct ggcgcagagc ttttacagct
    12481 agagaggcaa aatgacacct gaatcggtca tgatgatggg gactgaagcg atgaaagtcg
    12541 cgctggcact ggctgccccg ctgttgctgg tggcgctggt cacgggcctt atcatcagta
    12601 ttttgcaggc cgccacccag atcaacgaaa tgacgctgtc gtttattcca aaaatcatcg
    12661 ccgtatttat tgccattatt attgccggac cgtggatgct caatctgttg ctggattacg
    12721 tccgcacctt gttcactaac ctgccgtata tcatcgggta gcccgtacaa tgatgcaggt
    12781 gacaagcgat caatggcttt cctggctaag tctgtacttc tggccgttac tgcgcgtgct
    12841 ggcgctcatt tctaccgcgc caattctgag cgaacgcagc gtgccgaaac gggtaaaact
    12901 gggtctggca atgatgatca cgttcgctat tgccccatca ttacctgcca acgatgttcc
    12961 tgttttttcg ttctttgctc tgtggctggc cgtgcagcag atcctgatcg gcattgcgct
    13021 tggttttacc atgcaatttg cctttgccgc tgtgcgaacc gctggcgaaa ttatcggtct
    13081 gcaaatgggg ctgtcatttg cgacgtttgt cgatccggcc agccatctca atatgcctgt
    13141 tttagcgcgt atcatggata tgctggcgtt actactgttc ctgacattta acggtcattt
    13201 atggttgatt tcactgctgg tcgatacctt tcacaccctg ccaattggtg gcgaaccgtt
    13261 gaacagcaat gcgtttctgg cactcaccaa agcagggagt ttgattttcc ttaacgggct
    13321 gatgctggcg ttaccgctca ttactctgct gctgacactg aatctggcat taggtttact
    13381 taatcgtatg gccccgcaat tatccatttt tgttattgga tttccattaa ctctgactgt
    13441 cggtatctct ttaatggcgg cattaatgcc gttaattgca cctttttgcg aacatttatt
    13501 cagtgagatg tttaatttgc tggctgatat tattagtgaa ttgccattaa tataattccg
    13561 taacgtttat catgttatcc taaggattat ccgaaaaata atacctgcga acatcttcca
    13621 ggatactcct gtggcgaaat atttgtttta agctcactca catatcgcaa catttacttt
    13681 actttaagac aattccaggc aaattataca acactttacg ggatagtaag tccgcctgaa
    13741 aaatcgcgag agtggcgcat taggtgaccc atgttgttcc gtttagtcat gatgaaatat
    13801 tcaggtaagg ggaattatcg ttacgcattg agtgagggta tgccatgtca acgattatta
    13861 tggatttatg tagttacacc cgactaggtt taaccgggta tctgttgagt agaggggtta
    13921 aaaaaagaga aatcaacgac attgaaaccg ttgatgacct tgccatagct tgtgattcac
    13981 agcgcccttc agtggtgttt attaatgagg actgtttcat ccatgatgcg tctaacagtc
    14041 agcacatcaa gcacatcatt aatcaacatc ccaatacgtt atttatcgtt tttatggcaa
    14101 ttgccaatgt tcattttgat gaatatctat tggtcagaaa aaatttattg ataagttcta
    14161 aatcgattaa accggaatct ctcgacgata tccttggcga tattctgaaa aaagagacaa
    14221 cgataacctc ttttttaaat atgccgacgt tatcattgag ccgaaccgaa tcgagtatgt
    14281 tgcgaatgtg gatggcaggt cagggaacga ttcaaatctc tgaccaaatg aatatcaaag
    14341 ccaagaccgt ttcatcgcat aaaggtaata ttaaacgtaa gatcaaaacg cataataaac
    14401 aggttatcta ccatgtcgtc cgactgacgg ataatgtgac taatggtatt tttgtcaaca
    14461 tgcgctaaca cattctgact ggtggtttcc caccagtcag gctgaataag attactctgc
    14521 tttctccaca aagataccgt cctgatgccc tgcttcatta aagaaccaga tgccgagcgg
    14581 gtagtcttcc agcgaaacca ggtacattgt gccttcacta aactcctcaa ctgccagtac
    14641 cacgccagga cgacgcggac cgccatccgt tttgactgtt acccgatcat tcaccttcat
    14701 cgttttcctc ctgtggcttt gtgccagtgt agaacaattt cgtttttctg gcagcgccag
    14761 gcgcgcgcga gtgctgattt tctcgacggt ctatacttaa gagatgccag gcggacttaa
    14821 cgactggcgg caacaacaga gtaacggttg cgaggaaaga tgatgaaaac cgcaaaagag
    14881 tacagcgata ccgcaaaacg tgaggtcagc gtcgatgtcg atgccctgct ggcggcgatc
    14941 aatgaaatta gcgaaagcga agttcatcgc agccagaacg attctgaaca cgttagcgtc
    15001 gatggacgtg aatatcatac atggcgtgaa ttggcggatg ccttcgaact ggatattcat
    15061 gacttcagcg tctctgaagt gaatcgttga atgcacaata aaaaaatccc gaccctgagg
    15121 gggtcgggat gaaacttgct taagcaagaa gcacttaaaa aattcgttac accaggaaat
    15181 ctgatgtgtt catcacctta tccgcaattt ttttcgctga caagaaaata ttcgcgatat
    15241 gaatgattaa cgctacttat gaatagaaat gtgacgtgcg tcaccccttg gtggctctgg
    15301 cgtcaacggg atcgctcccg aaaaaatcat ctttgattat ttgtgctgtt ttgagaatca
    15361 tcctggtaag gggagaactg cgcgaccatg ttttcaattc aacaaccact actggttttt
    15421 agcgatcttg atggcaccct gctggacagt catagttatg actggcaacc ggctgccccc
    15481 tggctcagcc gtttacgcga agcgaatgtt cccgtcattc tctgtagcag taaaacttca
    15541 gcggaaatgc tgtacttgca aaaaacgttg gggctacaag ggttaccact gattgccgaa
    15601 aatggcgcag tgatccagct tgctgaacaa tggcaggata tagacggttt tccacgcatc
    15661 atctcaggta ttagccatgg cgaaatcagc caggttttaa atacgctacg cgagaaagaa
    15721 cattttaaat tcacgacttt cgatgatgtc gacgatgcaa ccatcgccga atggacggga
    15781 ttaagccgta gccaggcggc gctgacgcag ctgcatgagg cgtcggtaac gctaatctgg
    15841 cgcgacagtg acgagcgtat ggcacaattt accgctcgtc tgaacgaact gggcttacag
    15901 tttatgcagg gtgcgcgctt ctggcacgta ctggatgcct ctgccggaaa agatcaggct
    15961 gccaactgga ttatcgcgac ctatcaacaa ttgtcaggca aacgcccaac cacacttggc
    16021 ctgggcgatg ggccaaacga tgcgccctta ctggaggtaa tggattacgc ggtgattgtg
    16081 aaagggctaa accgtgaagg ggtgcatctg catgatgagg atccggcccg cgtctggcga
    16141 acgcagcgtg aaggaccgga aggctggggt gaagggctgg accatttttt ctccgcccgt
    16201 taagcgttat cgctcgcgca tacccgatta cgcccggcct gtttagcgag ataaagtcga
    16261 cggtcagcca gtgactggag ttgttcaaaa tcataatcac cggtttcctc gctgctactt
    16321 acccccagcg aggcactgat gcgtatcgtc gtactcttag caatcaacat ctctttttca
    16381 tttaacttca ggcgaatacg ttctgcgact tccgcagcct ccgtcagact cgcgcctggc
    16441 agaatcacac aaaactcctc accaccgacc cgcccggcaa cgtcctgcgc acgcaaggaa
    16501 ctgctaatta atccggcagc atgagaaaga acacggtcgc ccgcctgatg accaaagcgg
    16561 tcattaatcg ctttaaaatg gtcaagatcg acctgaatga cagaaaaagg atgttggtgc
    16621 gtctgataca atttagcgag cggacgggct ttttcgaaca gtgcgccacg gttatataaa
    16681 cgcgttaagg tgtcgtgcca cgcctgccac tgcaacgagc tttgcagaac atacatgttg
    16741 ctaaccatcc ggcgaatcac ataccaggag atgagtaaca tggtggtaaa gagcgcccac
    16801 agcagggtta atgcaatgct gatactgccg aaatcgccgc gcacgccttc gcttaacgta
    16861 tggacgcgca ccagcacacc gtcgaaatgg tccagacgtt cccagctaac atagcgactg
    16921 tccatacgaa tgccgccccg cgtgtcatgt tccatcgcct gcgccagcaa ggccagttca
    16981 cgaggatcaa aaatattccc tgttggatgg tcaggattgg aagaggtcaa aaatctcagc
    17041 ttactgtcat agagctgata ctcaccatcg aggtttttat cgatggcgtt tcttaaaaat
    17101 tgctgcatgg tacgcacggg aatactcatc cccagcacgc cataccagta gttattactg
    17161 tctactggaa cactgacggt aacctgcggt tcagtattgc tggcgtgttc cggttgcgaa
    17221 gtaaaccagc gtaccgcgcg gtgacgattt tctcgttgcg aatggccgat aaaccagggt
    17281 tgggtgacat agccgtaata acgcgttggt acattgcgcg taaacaaggt cggctgcgtc
    17341 gaaacgtaaa atccggcacg cgagacatac atcgcctgtt caaccatcga cgaggtgttg
    17401 tgcgccagtc gcagcaagta accaacttcc agtgcagcgg taatttcatt gtcgaggctt
    17461 tcattttcgc gagacaggag attcccctcg ctgactaatg catccgatac gccattgact
    17521 gacagggtgc gtcgtcggtt gagttcgatt ttccaggcgt gctcgtcgcg atgctgttcg
    17581 aactcggtta cagcatcacg cagagaggtg aaatccagtg gcgctacgag agcttcgcgc
    17641 atgccattac gcaaaaagat cagtttgtcg acgttatact gcaaatgctt atcgagcgcg
    17701 ttggctacgt tttccagatg attacgctgg ctggagatat aggcatcttc cagcaccact
    17761 acttcacgcc aggtgagcaa ggtggaaaaa agcaatacca cgataaagca gagattaacg
    17821 acatgaccag gccccaggcg gcgtgcgagt tttttcaacc agctctggtt ttccattttt
    17881 gtctcgtgct gcaccctgac gactcccaac ctgctttttt atgattctgg cataacctgg
    17941 cgatagcgta ccgcaaaaca gcgataattt tataatggtg gcatgataag acgcgacaga
    18001 catcgatagt tttataacgt aggcatgata agacgcgata gcgtcgcatc aggcaattca
    18061 gtgcagacac tggcacaccc gaccggatgc gacgtgagcg ccacatccgg tcgggtgtta
    18121 atgataccct ttcaaacttc ttccgcagga cgacttccct cccctggaac aagctcggtt
    18181 tcatgaaaag cttcccgctt gaccccgtaa ccgtcatacc aacgacactc aaccataccg
    18241 ctggagtatc cggtgacaat catccgcggg ccgccctctt taaccgtaac ttcctcacta
    18301 accataaagc tcatactcgc ctcctttttt cgagtgaaac ttgttcacct tagttgaaga
    18361 tggcgaattt tgcataatag ccagtgcgag tattaatgtg cctgaccgcg tattttcgct
    18421 acggctttca ctcccagcac cacgatgccg ccgatgataa atccaagaat cagatttaaa
    18481 acagtcggta atatcatcgc caccactgca ctttgctgcc cggcgaaatg ttcaatggcg
    18541 tgatgcagcg gcgcaatacc atgtaccaca atcccgccgc cgacgaggaa catcgccagc
    18601 gtgccgacaa tcgataacgc cttcatcaac cagggcgcaa tgatcaataa ccctttacct
    18661 aatgcctgca tcagcgcgct ggatttttcc gccagccaat accccaggtc atcaatctta
    18721 acgataaccc ctaccagacc gtagacgccc acggtcacta ccagcgcgat gcctgaaagc
    18781 accagcacct gattaagcaa cggcgcttcg gccacaatcc ccagcgtgat ggcgacgatt
    18841 tcggcagaca atataaaatc ggtacgaatc gcccctttta ctttgtcctt ttcaaacttc
    18901 agcggatcct gcgccgccag cttctccaga cgctgctggc tctgcgccgg atcttcttta
    18961 tgtttacgcg cctccagcat atgcagcact ttctctactc cttcaaagca gagaaacgcg
    19021 ccaccaatca tcaacagagg cgtaatcgcc cacgggataa acgcactgat gatcagcgcc
    19081 agcggcacca gaatcacttt attaatcagc gatcctttcg ccacgcccca gaccacggga
    19141 agttcccggt tggcccgcac gcctgaaact tgttgcgcat tgagcgataa gtcatcccct
    19201 aatacaccgg cggttttctt cgccgccagt ttgcccatca cggagatatc gtccagtagt
    19261 gtggcgatat catcgagcaa cgtcagtaaa ctactcccgg ccaaaagcat tccctctcat
    19321 ttttattgtt gaagggtgta agtatgaagc aaaaacggca gctctgatac ttgcttcaca
    19381 ggtcaacatt tttttaacaa ttaagtgcaa ttatatctct cgccagcaga atgattttca
    19441 cgtttactat gaaccacctt tttatttcca ccgtgaggga ttatgcgttt ccgccagttg
    19501 ttaccgcttt ttggcgcgct gtttgcgttg tatatcattt ggggctcaac ctattttgtc
    19561 attcggattg gcgtggaaag ctggcctccg ttaatgatgg caggcgttcg attcctggca
    19621 gccggtattt tattgatggc atttttgcta ctgcgcggac acaagctccc cccgctacgt
    19681 ccgctgctca atgccgcgct gattggcctg ttattgctgg ctgtcggtaa tggcatggtg
    19741 acggttgccg agcatcaaaa tgttccttcc ggcatcgccg ccgttgtggt tgcaaccgtg
    19801 cccctcttta ccctgtgctt cagccgcctg tttggcatta aaacgcgcaa actggaatgg
    19861 gtgggtatcg ccattgggct tgccggaatc atcatgctca atagcggtgg aaatttaagc
    19921 ggcaatccgt ggggcgcgat tctgatttta atcggctcga ttagctgggc gtttggctcg
    19981 gtttatggct cgcgcattac cttacctgta gggatgatgg cgggtgctat tgagatgctg
    20041 gcggcaggcg tggtgttaat gatcgcgtcg atgattgcag gtgaaaaact gacggcgctc
    20101 ccttcccttt caggcttcct tgcggtcggc tatctggcgc tgtttggttc gattatcgcc
    20161 atcaacgctt atatgtattt aatccgtaat gtcagtccgg ctctcgccac cagctacgcc
    20221 tacgttaacc cggtggtcgc ggtcttgctg ggtacgggac tgggcggaga gacgctgtcg
    20281 aagattgaat ggctggcgct cggcgtaatt gtcttcgcgg tggtgctggt cacgttggga
    20341 aaatatctct ttccggcaaa acccgtagtt gcgccagtta ttcaggacgc atcaagcgag
    20401 taaatgaatc ccctgcgtgt cgatctgcgc gctggcacct tcgccgcaga tccactcttc
    20461 cagacgctcg ctaagcgctt catccgtcag cttctcgcgc ccacgtaacg cgcactccca
    20521 gacaatcaat acgcgccagc cgagttcctg caagcgactg atatcgcggc gatcgcgctc
    20581 aacattttta cctatcttct ccagccagaa ctcggttcga gtcgcaggca ctttaaacag
    20641 atagcagtga tgatgatgcc agaagcagcc gtgggtaaat atcacgcagc gatattcatc
    20701 aacgacaaaa tccgggcgtc cgggcagact ggcgtcctga acgcgaaacg ccaggccctg
    20761 cccggttaac agactggcga ggcgcttctc tatcgccgta tcacgcgtgg caatcgcgcg
    20821 catatttttg ctgcgagtgg ccttatcgtg aacgtcggcc atgttgtgcc tcttgctgac
    20881 gcaacgccac cgcctgtttg atttttggct caagcagttt tgccaccgcg gcaaagactg
    20941 gcacgaccac cgagttaccg aactggcgat aggcctgagt gtccgaaacc ggaatgcgaa
    21001 atttcgcttc tcccggcgct tcaaaaccca ttaagcgcgc gcattcacga ggcgttaacc
    21061 gacgtggacg atgttgctga ttctgcggat cgtcaaagtc tttctcacct ttggccatat
    21121 cccagccgcg atcgattaaa atttccgcgc catctttgta ataacgcgca gacagcgtac
    21181 gcgtgacgct ttgcggattg ttcggataaa ccattccata accgaagccg ttaccgcgcg
    21241 cctgatgttt tttcgcatat cgatagaggt acttccacag caccggcgtc aggatatatt
    21301 tcgcctcgac catcgggtcc agcagctgcg ccagcgtcac tcgctgcgca gggaaacatt
    21361 cgctgatatc acgcagggta aaatcggctt tcagattcag atcgcgacga aaacccacca
    21421 gcacgatgcg ttcacggtgc tgcggcagaa aatgtttgcc gtcgatgatt ttcggatcgt
    21481 ctggcccgtt atcttctgca tcagccacgt catagcccag ttcgtccagc gtctgcatga
    21541 tgatgcggaa cgttttaccc tggtcgtgac ttttcaggtt tttgacgttt tcgagcacaa
    21601 acatcgccgg acgacgcgcg tcgataatgc gcaccacatc aaagaacagc gtgccctgag
    21661 tatcgcaggc aaaaccgtgc gcccgcccga gcgagttctt tttcgatacg ccagccagcg
    21721 aaaatggctg acaagggaaa ccggccagta aaacatcgtg ttcaggaatg tgttgacgaa
    21781 tatgttccgc cgccgcctca tcactcacgc cttctttatg gctgagggtg atgtcgcgga
    21841 tatcttcatt aaaatgatgc gtcgccggat cgcaataatg gttggcttta taagtgcgta
    21901 ccgcatgttt gttccattcg ctggtaaaca cgcactgtcc gccaatcgat tcaaaaccgc
    21961 gacggatgcc gccaattccg gcgaatagat cgataaagcg aaacgcataa tgcggatgat
    22021 gtgccggtgg tttgggtaat aacgtttgca gatgggcgaa ctctttctca cttaaacggt
    22081 gccatgccga gtcattcgcc agcgcacgtt ttaaaattgc cgcgctccag tgattctcac
    22141 ctacaccatt aagctgcgcc accagcgttt taacatcata aatttgcagc agtttctcca
    22201 gcattgcctg tgcggcattc ccggtgctgt atgaatcggt tactgatata ttttcctgca
    22261 tagatttcac cggccatctg agatggccga caggttaaca taattacagc catttagggc
    22321 cacgcgctca gccagcggat gaaaacgcat ctatcacctt gtgatcgaca ccttcattct
    22381 cccccgccag ttcggcactg agcttcgcca taaactcgac taaaaaatgc gcgttgtgct
    22441 gcgccagctg cttgcctctt gcggtttgca tggtttgcgg tagcttcagc aacttagtct
    22501 ggaaatgatc cagcgcgtag cgtttatcat caagcgggcg atgctgtgcg aacggatctt
    22561 caccatcaaa cagcgccacg cccaatgccc ctgaaacggc aaacacgcgc gccaggccaa
    22621 tcgcccccag ggcttccagc cgatccgcat cctggacgat tttagcttcc gttgttaagg
    22681 gggcgatttg cgcgctgaaa ctgtgagcgg caatggcatg acaaacggcc tcgattttct
    22741 ccgccggaaa ttgctcgaac tcttcacgga gcaggcgacg cgtctcttct gccgccagga
    22801 ttgaagaacg ctgcctttgc ggatgatttt tcgccaggct cacaatatcg tgaaaataac
    22861 aggcggttaa aatcaccagc atatcgacat cgtcatctgc cgccagcttc tgtgccgtag
    22921 cccagacgcg gcgaaaatga cacacgtcat gggccgcgtc ctggtgctga tgatggtttt
    22981 tcaaccagtt ttcgaactgt gcctgccagt gttgtaagtc catacgcgct ccgttgccaa
    23041 agagtcgcca ccttagcagt ttttattcat agcgtctgct acgtgcggga cgaggtcggg
    23101 tgtaccaggc aatgctgcct aacaatgccc cgaccagagc cataaataaa aatccgacca
    23161 gtgcccctaa ccactttccc gcggtagagc ctaaatcgct ttcaaatccc gacacggggg
    23221 catcggggat ccggctcatc acccagatgt aacgcgccat tgcccagacg aaatagccac
    23281 accatgtata gaaagcccac aaagccagtt tgccgccagg gctgcgagag agtctctctt
    23341 ccatctttgt cgaaccgtat tccattttga gataacccgg aaaattcaga cttagtacta
    23401 ttatgtgata aaagtcacat ttttccacat tggatgagcc agtcaagact cactcatttt
    23461 ttgaacaaat agataattt
//
LOCUS       DAZGVQ010000076        23036 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000076 DAZGVQ010000000
VERSION     DAZGVQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.076"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1941)
                     /gene="speA"
                     /locus_tag="ACHLAG_002852"
     CDS             complement(<1..1941)
                     /gene="speA"
                     /locus_tag="ACHLAG_002852"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFP0656044.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEA"
     gene            complement(1950..2081)
                     /gene="yqgB"
                     /locus_tag="ACHLAG_002853"
     CDS             complement(1950..2081)
                     /gene="yqgB"
                     /locus_tag="ACHLAG_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFP0656045.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(2175..2318)
                     /gene="yqgG"
                     /locus_tag="ACHLAG_002854"
     CDS             complement(2175..2318)
                     /gene="yqgG"
                     /locus_tag="ACHLAG_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFP0656046.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(2429..2680)
                     /gene="yqgD"
                     /locus_tag="ACHLAG_002855"
     CDS             complement(2429..2680)
                     /gene="yqgD"
                     /locus_tag="ACHLAG_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFP0656047.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            2736..3890
                     /gene="metK"
                     /locus_tag="ACHLAG_002856"
     CDS             2736..3890
                     /gene="metK"
                     /locus_tag="ACHLAG_002856"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFP0656048.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4326..5720
                     /gene="galP"
                     /locus_tag="ACHLAG_002857"
     CDS             4326..5720
                     /gene="galP"
                     /locus_tag="ACHLAG_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFP0656049.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5797..6294
                     /gene="yggI"
                     /locus_tag="ACHLAG_002858"
     CDS             5797..6294
                     /gene="yggI"
                     /locus_tag="ACHLAG_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFP0656050.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            6389..7096
                     /gene="endA"
                     /locus_tag="ACHLAG_002859"
     CDS             6389..7096
                     /gene="endA"
                     /locus_tag="ACHLAG_002859"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFP0656051.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7176..7907
                     /gene="rsmE"
                     /locus_tag="ACHLAG_002860"
     CDS             7176..7907
                     /gene="rsmE"
                     /locus_tag="ACHLAG_002860"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFP0656052.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            7920..8870
                     /gene="gshB"
                     /locus_tag="ACHLAG_002861"
     CDS             7920..8870
                     /gene="gshB"
                     /locus_tag="ACHLAG_002861"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFP0656053.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            8979..9542
                     /gene="yqgE"
                     /locus_tag="ACHLAG_002862"
     CDS             8979..9542
                     /gene="yqgE"
                     /locus_tag="ACHLAG_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFP0656054.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9542..9958
                     /gene="ruvX"
                     /locus_tag="ACHLAG_002863"
     CDS             9542..9958
                     /gene="ruvX"
                     /locus_tag="ACHLAG_002863"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFP0656055.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10134..11114)
                     /gene="yggR"
                     /locus_tag="ACHLAG_002864"
     CDS             complement(10134..11114)
                     /gene="yggR"
                     /locus_tag="ACHLAG_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFP0656056.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            11132..11836
                     /gene="yggS"
                     /locus_tag="ACHLAG_002865"
     CDS             11132..11836
                     /gene="yggS"
                     /locus_tag="ACHLAG_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFP0656057.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPSLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11854..12420
                     /gene="yggT"
                     /locus_tag="ACHLAG_002866"
     CDS             11854..12420
                     /gene="yggT"
                     /locus_tag="ACHLAG_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFP0656058.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            12417..12707
                     /gene="yggU"
                     /locus_tag="ACHLAG_002867"
     CDS             12417..12707
                     /gene="yggU"
                     /locus_tag="ACHLAG_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFP0656059.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12715..13308
                     /gene="rdgB"
                     /locus_tag="ACHLAG_002868"
     CDS             12715..13308
                     /gene="rdgB"
                     /locus_tag="ACHLAG_002868"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFP0656060.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDNQRQARFHCVLVYLRHAEDPTPLVCHGSWPGMITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13301..14437
                     /gene="hemW"
                     /locus_tag="ACHLAG_002869"
     CDS             13301..14437
                     /gene="hemW"
                     /locus_tag="ACHLAG_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFP0656061.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(14592..15599)
                     /gene="yggM"
                     /locus_tag="ACHLAG_002870"
     CDS             complement(14592..15599)
                     /gene="yggM"
                     /locus_tag="ACHLAG_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFP0656062.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(15716..16762)
                     /gene="ansB"
                     /locus_tag="ACHLAG_002871"
     CDS             complement(15716..16762)
                     /gene="ansB"
                     /locus_tag="ACHLAG_002871"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFP0656063.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(16938..17657)
                     /gene="yggN"
                     /locus_tag="ACHLAG_002872"
     CDS             complement(16938..17657)
                     /gene="yggN"
                     /locus_tag="ACHLAG_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFP0656064.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(17678..17818)
                     /locus_tag="ACHLAG_002873"
     CDS             complement(17678..17818)
                     /locus_tag="ACHLAG_002873"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656065.1"
                     /translation="MQNCLMRYAYHAWKDARSSRPDKAFTPHPAWKTRTLLSIWGQQM
                     LA"
     gene            complement(17841..18167)
                     /gene="yggL"
                     /locus_tag="ACHLAG_002874"
     CDS             complement(17841..18167)
                     /gene="yggL"
                     /locus_tag="ACHLAG_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFP0656066.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(18167..18886)
                     /gene="trmB"
                     /locus_tag="ACHLAG_002875"
     CDS             complement(18167..18886)
                     /gene="trmB"
                     /locus_tag="ACHLAG_002875"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFP0656067.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            19047..20099
                     /gene="mutY"
                     /locus_tag="ACHLAG_002876"
     CDS             19047..20099
                     /gene="mutY"
                     /locus_tag="ACHLAG_002876"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFP0656068.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHSFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            20127..20402
                     /gene="yggX"
                     /locus_tag="ACHLAG_002877"
     CDS             20127..20402
                     /gene="yggX"
                     /locus_tag="ACHLAG_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFP0656069.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            20467..21546
                     /gene="mltC"
                     /locus_tag="ACHLAG_002878"
     CDS             20467..21546
                     /gene="mltC"
                     /locus_tag="ACHLAG_002878"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFP0656070.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRNNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            21748..23004
                     /gene="nupG"
                     /locus_tag="ACHLAG_002879"
     CDS             21748..23004
                     /gene="nupG"
                     /locus_tag="ACHLAG_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFP0656071.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
BASE COUNT         5475 a         6071 c         5810 g         5680 t
ORIGIN      
        1 tgcctcgaac tcttcaagga actgttgttg cagttcagca tcaagatcgg ttttcttcac
       61 ttgatcacgg aactgagtta acagcgtttt cggatcgagc tgtacatatt gcagcatgtc
      121 cgccacggta tcgccttcgt cagacagttc cacttcgaca ctaccgtcag ggaacacgaa
      181 cacgtcaacc gcttcggtat caccgaacag gttgtgcatg ttgccgagga tctcctgata
      241 tgcgccgacc ataaagaaac cgagcatcgg cggattctct ggatcgtact ccggcatagg
      301 cattgtcgtg gcaataccgt caccatcaat atagtggtcg atagcaccgt cagagtcaca
      361 ggtaatatcc agcagcacag cgcggcgttc cggcacttga tccagccctt ccagcggcag
      421 aaccgggaac aactggtcga tcccccacgc atccggcatc gactggaaca gcgagaagtt
      481 gacgtacatt ttgtccgcca tacgttcctg cagctcgtcg ataatcggac gatgagcacg
      541 gttttgcgga tccagctgct tttgcacttc atggcacatg ctcaaataaa gctgctcagc
      601 ccatgcgcgt tcttgcaggc taaaggtgcc ggaagagtag ccgatatgaa tgtcgtgcag
      661 atccatctga ctgtcgtgta accattcacg cagagaacgg cgagttcccg gttcgtgcat
      721 ctcctgccag gtttcccaca tgctttgcag cgcacgcggc gcatcttctg caggcgcggt
      781 cggcaccgtg tattcgttac gttccacgcc gatgatatta gacaccagca cggtgtgatg
      841 cgcagtcact gcacgacccg attcagtgat taccgtcgga tgcggcagac cattttcttc
      901 acacgcatcg ccaatcgccc agatgatgtt gttggcgtat tcattgaggc catagtttac
      961 ggagcagtca gactgcgaac gggtgccttc atagtccacg cccagaccgc cgccgacgtc
     1021 gaagcactga atattgacgc ccagcttgtg cagttccaca tagaaacgcg ccgattcacg
     1081 aacgcctgtg gcgatatcgc gaatattcgc catctgcgaa ccgaggtgga agtgcagtag
     1141 ttgcaggctg tcgagacgcc cggcttcacg cagggtttca accagttgca gtacctgagt
     1201 cgcagccagg ccgaacttcg atttttcccc gccggaggac tgccatttac ccgagccctg
     1261 cgaagccaga cgtgcacgca cgcccagacg aggaacgaca ttcagacgtt ctgcttcatc
     1321 cagcacaatg gcgatttctg acatcttctc aatgaccaga tagaccttgt gccccatctt
     1381 ctcgccaatt aatgccaggc ggatatattc gcggtcttta taaccgttgc agacgatgac
     1441 gctacgggtc atgccagcat gcgccagcac cgccatcaac tctgctttcg agcctgcttc
     1501 cagccccaac ggttcgccag aatgaatcag ggactcaatc acgcggcggt gctggttaac
     1561 tttgatcgga taaacaagga agtaatcgcc gttatagccg taggattccc gcgcacgttt
     1621 gaacgcggcg ttaatggaac gcaaacggtg ctgcaggatc tgtgggaaac agaacagtgc
     1681 aggcagacgc tgaccctgtg cttcacgagt tttcactaac tgcgcgagat cgacgcgagc
     1741 ttccgggacg tccgggtccg ggcacacgct gatgtggccc agctcgttaa cgtcatagta
     1801 gttattgccc caccaggcaa tattgtaagt acgcagcatc ttgctggctt cctgggagct
     1861 cattgcaacc tcctgcatgg agcgtagtac accgtgttcg cccgctgacg aaggcaaacc
     1921 catagacatg tcgtcagaca tagcgaacct caaattattt tattaagtgt aaaacagtta
     1981 acgactatcg cagcctggaa ctgcgataac aacccataag cacatggatt ttccagcagt
     2041 gaatgctgac gctccaactg cgcaaccggt ttctttttca taacattatt aagcacataa
     2101 ccgaacgtaa gtgtgacagt tcggcgaaac cacgagaaaa ctcttgtttt tacaagagcg
     2161 cccttgttca gtcctcagta actgtaacca gctcttgaat cctgagaagc gccgagatgg
     2221 gtataacatc ggcaggtatg caaagcagag atgcagagtg cggggaacga atcttcacca
     2281 gaacggtgag acaggttaag cagcagacaa cggttcatta tttcgtatca cctccacggc
     2341 cgcctgttaa gacgaaccca caagccaaaa ctctgatttc aacccggctg gaagtggcaa
     2401 cacgaaagaa acgtcgtgtg ctttttattt aagccgcgcg ccgcgtttta taccccacaa
     2461 tggcagaaaa ttgcaaaaga taaatacgca gaatgccggc attgtcagga aaaatttcca
     2521 gccacgtttt taacagaatg agacacgatt caaaaaaaag tggaaatcgg gtgaagaatt
     2581 gacctaaaat agccatccag atgttaatcc atccataccg attaacactc agactgccag
     2641 tgtttttaac ttgcagagtc gtggtaggat ccgctaccac agaaaatcca cacaacagtt
     2701 tgagctaacc aaattctctt taggtgatat taaatatggc aaaacacctt tttacgtccg
     2761 agtccgtctc tgaagggcat cctgacaaaa ttgctgacca aatctctgat gccgttttag
     2821 acgcgatcct cgaacaggat ccgaaagcac gcgttgcttg cgaaacctac gtaaaaaccg
     2881 gcatggtttt agttggcggc gaaatcacca ccagcgcctg ggtagacatc gaagagatca
     2941 cccgtaacac cgttcgcgaa attggctatg tgcattccga catgggcttt gacgctaact
     3001 cctgtgcggt tctgagcgct atcggcaaac agtctcctga catcaaccag ggcgttgacc
     3061 gtgccgatcc gctggaacag ggcgcgggtg accagggtct gatgtttggc tacgcaacta
     3121 atgaaaccga cgtgctgatg ccagcaccta tcacctatgc acaccgtctg gtacagcgtc
     3181 aggctgaagt gcgtaaaaac ggcactctgc cgtggctgcg cccggacgcg aaaagccagg
     3241 tgactttcca gtatgacgac ggcaaaatcg ttggtatcga tgctgtcgtg ctttccactc
     3301 agcactctga agagatcgac cagaaatcgc tgcaagaagc ggtaatggaa gagatcatca
     3361 agccgattct gcccgctgaa tggctgactt ctgccaccaa attcttcatc aacccgaccg
     3421 gtcgttttgt tatcggtggc ccgatgggtg actgcggtct gactggtcgt aaaattatcg
     3481 ttgataccta cggcggcatg gcacgtcacg gtggcggagc attctctggt aaagatccat
     3541 caaaagtgga ccgttccgct gcgtacgcag cacgttatgt tgcaaaaaac atcgttgctg
     3601 ctggcctggc cgatcgttgt gaaattcagg tttcctacgc aatcggcgtg gctgaaccga
     3661 cctccatcat ggtagaaact ttcggtactg agaaagtgcc ttctgaacaa ctgactctgc
     3721 tggtacgtga gttcttcgac ctgcgcccat acggtctgat tcagatgctg gatctgctgc
     3781 acccgatcta caaagaaacc gcagcatacg gtcactttgg tcgtgaacat ttcccgtggg
     3841 aaaaaaccga caaagcgcag ctgctgcgcg atgctgccgg tctgaagtaa tctttcttca
     3901 cctgctttca aaggccagcc tcgcgctggc ctttttcttt tacctcaccg cggtggatag
     3961 gcattcacgc cgcatccggc aaaaagctgc ccgcacaata acatcattct tcctcaacac
     4021 gttttatcgc aaattatcat cactactgaa accgattaca ccaataacac caaccaaata
     4081 tttgtaatat tttcatatta ttattcagtt ttcacagttg ttacatttct tttcagtaaa
     4141 gtcttaattg cagataacag cgtttaatct atgatgatat aactcaatta ttttcatgca
     4201 cttaaaccat aactaagata aatgttagtg taagcgatta cactgatgtg atttgcttca
     4261 catcttttta cgtcgtactc acctatctta attcacaata aaaaataacc atattggagg
     4321 gcatcatgcc tgacgctaaa aaacaggggc ggtcaaacaa ggcaatgacg tttttcgtct
     4381 gcttccttgc cgctctggcg ggattactct ttggcctgga tatcggtgta attgctggcg
     4441 cactgccgtt tattgcagat gaattccaga ttacttcgca cacgcaagaa tgggtcgtaa
     4501 gctccatgat gttcggtgcg gcagtcggtg cggtgggcag cggctggctc tcctttaaac
     4561 tcgggcgcaa aaagagcctg atgatcggcg caattttgtt tgttgccggt tcgctgttct
     4621 ctgcggctgc gccaaacgtt gaagtactga ttctttcccg cgttctactg gggctggcgg
     4681 tgggtgtggc ctcttatacc gcaccgctgt acctctctga aattgcgccg gaaaaaattc
     4741 gtggcagtat gatctcgatg tatcagttga tgatcactat cgggatcctc ggtgcttatc
     4801 tttctgatac cgccttcagc tacaccggtg catggcgctg gatgctgggt gtgattatca
     4861 tcccggcaat tttgctgctg attggtgtct tcttcctgcc agacagccca cgttggtttg
     4921 ccgccaaacg ccgttttgtt gatgccgaac gcgtgctgct acgcctgcgt gacaccagcg
     4981 cggaagcgaa acgcgaactg gatgaaatcc gtgaaagttt gcaggttaaa cagagtggct
     5041 gggcgctgtt taaagagaac agcaacttcc gccgcgcggt gttccttggc gtactgttgc
     5101 aggtaatgca gcaattcacc gggatgaacg tcatcatgta ttacgcgccg aaaatcttcg
     5161 aactggcggg ttataccaac actaccgagc aaatgtgggg gaccgtgatt gtcggcctga
     5221 ccaacgtact tgccaccttt atcgcaatcg gccttgttga ccgctgggga cgtaaaccaa
     5281 cgctaacgct gggcttcctg gtgatggctg ctggcatggg cgtactcggt acaatgatgc
     5341 atatcggtat tcactctccg tcagcgcagt atttcgccat cgccatgctg ctgatgttta
     5401 ttgtcggttt tgccatgagt gccggtccgc tgatttgggt actgtgctcc gaaattcagc
     5461 cgctgaaagg ccgcgatttt ggcatcacct gctccaccgc caccaactgg attgccaaca
     5521 tgatcgttgg cgcaacgttc ctgaccatgc tcaacacgct gggtaacgcc aacaccttct
     5581 gggtgtatgc ggctctgaac gtactgttta tcctgctgac attgtggcta gtaccggaaa
     5641 ccaaacacgt ttcgctggaa catattgaac gtaatctgat gaaaggtcgt aaactgcgcg
     5701 aaatcggcgc tcacgattaa tctcctcagg cttcctcccg tcgcggagga agccacctct
     5761 tgcagtcatc ttttcttcgc tctatcctct gccgctatga aaacatcccg tctccctatc
     5821 gccatccaac aggccgttat gcgccgcctg cgggaaaaac tcgcccaggc caacctgaag
     5881 ctagggcgta actacccgga gccaaaactc tcttacaccc agcgcggaac ctccgccgga
     5941 acggcctggc tggaaagcta tgaaattcgc ctcaatcccg ttttgctgtt ggaaaacagt
     6001 gaagctttta ttgaagaagt ggtaccgcac gaactggcac atttgctggt atggaaacat
     6061 ttcggccgcg tagcgccaca tggcaaagag tggaagtgga tgatggaaag cgtgctgggt
     6121 gttcccgccc gtcgtacgca tcagttcgaa ctgcaatccg tgcgtcgcaa caccttcccc
     6181 taccgctgca agtgccagga gcatcagctt accgtacgcc gccataatcg cgtagttcgt
     6241 ggcgaggccg tctatcgctg tgttcactgc ggtgaacagc tggttgcgaa ataaccatct
     6301 gaactatcag gaactttcct gatctggctg attgcatacc aaaacaactt tcgctacgtt
     6361 gctggctcgt tttaacacgg agtaagtgat gtaccgttat ttgtctattg ctgcggtggt
     6421 actgagcgca gcattttccg gcccggcgtt ggccgaaggt atcaatagtt tttctcaggc
     6481 gaaagccgcg gcggtaaaag tccacgctga cgcgcccggt acgttttatt gcggatgtaa
     6541 aattaactgg cagggcaaaa aaggcgttgt tgatctgcaa tcgtgcggct atcaggtgcg
     6601 caaaaatgaa aaccgagcca gccgcgtaga gtgggaacac gtcgttcccg cctggcagtt
     6661 cggtcaccag cgccagtgct ggcaggacgg tggacgtaaa aactgcgcta aagatccggt
     6721 ctatcgcaag atggaaagcg atatgcataa cctgcagccg tcagtcggtg aggtgaatgg
     6781 cgatcgcggc aactttatgt acagccagtg gaatggcggc gaaggccagt acggtcaatg
     6841 cgccatgaag gtcgatttca aagaaaaagc tgccgaacca ccagcgcgtg cacgcggtgc
     6901 cattgcacgc acctacttct atatgcgcga ccaatacaac ctgacactct ctcgccagca
     6961 aacgcagctg ttcaacgcat ggaacaagat gtatccggtt accgactggg agtgcgagcg
     7021 cgatgaacgc atcgcgaagg tgcaaggcaa tcataatccg tatgtgcaac gcgcttgcca
     7081 ggcgcgaaag agctaaccta cactagcggg actctttttg ttaaccccta ccccacgcgt
     7141 acaaccgcgt ggggagacga cgcggatttt taactatgcg tatcccccgc atttatcatc
     7201 ctgaaccact gaccagccat tctcacatcg cgctttgcga agatgccgcc aaccatatcg
     7261 ggcgcgtact gcgcatgggg ccggggcagg cgttgcaatt gtttgacggt agcaaccagg
     7321 tctttgacgc cgaaattacc agcgccagca aaaaaagcgt ggaagtgaag gtgctggaag
     7381 gcaagatcga cgatcgcgaa tctccgctgc atattcacct cggtcaggtg atgtcgcgtg
     7441 gtgaaaaaat ggaatttacg atccagaaat cgatcgaact cggtgtaagc ctcattacgc
     7501 cacttttttc tgagcgctgc ggcgttaaac tggatagtga acgtctgaac aagaagctcc
     7561 agcagtggca gaagattgca attgctgcct gtgagcagtg tggtcgtaac cgggtgccgg
     7621 aaatccgccc agcgatggat ctggaagcct ggtgtgcaga gcaggatgaa ggactgaaac
     7681 tgaatcttca cccgcgcgcc agtaacagca tcaatacgtt gccgttaccg gttgaacgcg
     7741 tccgcctgct gattggcccg gaaggcggtt tatcggcaga tgaaattgcc atgactgccc
     7801 gctatcaatt tactgatatc ctgttgggac ctcgcgtttt gcgtacagag acaactgcgc
     7861 tcaccgccat taccgcgcta caagtacgat ttggcgattt gggctaacgg agaagaataa
     7921 tgatcaagct cggcatcgtg atggacccca tcgcaaacat caacatcaag aaagattcca
     7981 gtttcgctat gttgctggaa gcacagcgtc gtggttacga acttcactat atggagatgg
     8041 gcgatctgta tctgatcaat ggcgaagccc gcgcccatac ccgcacgctg aacgtgaagc
     8101 agaactacga agagtggttt tcattcgtcg gtgaacagga tctgccgctg gccgatctcg
     8161 atgtgatcct gatgcgtaaa gacccgccgt ttgataccga gtttatctac gcgacctata
     8221 ttctggaacg tgccgaagag aaagggacgc tgatcgttaa caagccgcag agcctgcgcg
     8281 actgtaacga gaaactgttt accgcctggt tctctgactt aacgccagaa acgctggtta
     8341 cgcgcaataa agcgcagcta aaagcgttct gggagaaaca cagcgacatc attcttaagc
     8401 cgctggacgg tatgggcggc gcgtcgattt tccgcgtgaa agaaggcgat ccaaacctcg
     8461 gcgtgattgc cgaaaccctg actgagcatg gcactcgcta ctgcatggcg caaaattacc
     8521 tgccagccat taaagatggc gacaaacgcg tgctggtggt ggatggcgag ccggtaccgt
     8581 actgcctggc gcgtattccg caggggggcg aaacccgtgg caatctggct gccggtggtc
     8641 gcggtgaacc tcgtccgctg acggaaagtg actggaaaat cgcccgtcag atcgggccga
     8701 cgctgaaaga aaaagggctg atttttgttg gtctggatat catcggcgac cgtctgactg
     8761 aaattaacgt caccagccca acctgtattc gtgagattga agcagagttt ccggtgtcga
     8821 tcaccggaat gttaatggat gccatcgaag cacgtttaca gcagcagtaa cccaccttag
     8881 cgagaaggat ctcgttgaga ctctgagtga cagcgccctt ctttccacgc atactgggcg
     8941 ctgttgcttt tttgaaccag gaaacagaac ctctgacaat gaatttacag catcactttc
     9001 ttattgccat gcctgctctc caggatccga ttttccgtcg ttccgtggtc tacatttgcg
     9061 aacataatac caatggtgca atggggatca tcgtcaacaa gccgctggaa aatctcaaaa
     9121 ttgaagggat tctggaaaag ctgaagatca cgccggagcc gcgtgatgaa tcaatccgtc
     9181 tggataaacc ggttatgctc ggcggtccgc tggctgaaga tcgcgggttt attttgcata
     9241 ctccgccctc caattttgct tccagcattc gcatttcaga caacacagtg atgaccactt
     9301 cccgcgatgt gctggaaacg ctcggcaccg ataaacaacc gtctgacgta ttggtggctc
     9361 tgggttatgc ctcctgggaa aaaggtcaac tggaacaaga aattctcgat aacgcgtggc
     9421 taacggcccc ggcggatctg aatattctgt tcaaaacgcc gattgccgac cgctggcgcg
     9481 aggcggcaaa actgattggt gtggatattc tcaccatgcc tggtgtggca gggcacgcct
     9541 gatgagtgga accttactcg ccttcgactt cggcaccaaa agcattggcg tagcggtcgg
     9601 ccaacgcatt accggcactg ctcgcccttt gcctgcaatt aaagcacagg acggtacgcc
     9661 ggactggaac ctcatcgaac gtttactgaa agagtggcag ccggacgaaa tcatcgtcgg
     9721 tttgccgctg aatatggacg gcaccgagca accattgact gccagagcgc gtaaatttgc
     9781 caaccgtatt catggtcgtt tcggtgttga agtaaagctc catgacgaac gtcttagcac
     9841 cgtggaagcc cgttccggtc tgtttgaaca gggcggctat cgggcgctca acaaaggcaa
     9901 agtcgactct gcctctgcgg ttattattct cgaaagctat ttcgagcagg gatattaagg
     9961 cgatctaaaa gcctggcgga tagtcgttac cgccatcttg ccggatgcgg cgtgaacgcc
    10021 ttatccagta cacatctggc agcggctgta ggtctgataa gacgcgttag cgtcgcatca
    10081 gacatttatt gcctttatag gccctataaa cttgcgcatc gggcatggca acgtcacaaa
    10141 cgcccttccc ccacccgctg ctgataactc tgctgaaacg ttaacatccc cacctgctgc
    10201 ccggtttgaa taacatgcgg taactggtgg gttttccctt cgcgaatcaa attccccacc
    10261 gcgggagtgt taatcaataa ttcaaacagc gccacgcgtc cttcctgttt atccacttcc
    10321 agcttttgtg acaacacggc ccgtaaacta cctgccagtt gattacgtac gggatctttt
    10381 tcctgcgccg gaaatgaatc caccagtcgc tcaactgcct gcgcggcacc acgcgtatgt
    10441 aatgttgcca gcaccaaatg cccggtttct gccgccgtca gtgccagacg gattgtctcg
    10501 ctgtcacgca gctctccgag caaaatcaca tcaggatctt cccgcaatgc ggcccgcaat
    10561 cccgatgcga acgtcataca gtgcaaacca atttcccgct gctggatcaa acatcgctgg
    10621 ctggcataga tatattcaac aggatcttcc agcgtcagaa tatgcgcatc ggcatgttga
    10681 ttgagatagc caaccatcgc cgccagcgtg gtagatttgc cgctccccgt cgcccccgtc
    10741 accagaatca ggccattctc gctcttgagt aattccggca ataccgttgg tgcgccaagc
    10801 tgttcgagct gtggacagtg cgaaggtaac aaccgtaatg ccagagaaat accttgccgt
    10861 tgcgcgaacg cactgccacg caaccgctgg ttttccgcca gcgacacggc aaaatccagc
    10921 tggccatttt ccagcaatat tgcccgctga tcgtcatcca gccactcccg cagtagctct
    10981 tcgacgtccg gcgcatcaaa cggcgcagct tccattagcc cgcgaatgcg ccatcgtgcg
    11041 ggccaggcgc tgcacaggtg tagatccgag acgttatgct ttacactaag ggccacaatt
    11101 tcttccatat tcatactaag atcctcggaa aatgaacgat attgcgcata acctggcaca
    11161 ggtccgggac aaaatctcag cggctgcaac gcgttgcggc cgttctccag aagaaattac
    11221 gctgcttgca gtcagtaaaa caaaacctgc gagcgccatc gcagaagcca ttgatgccgg
    11281 gcagcgtcaa tttggtgaaa actacgttca ggaaggggta gataaaattc gccactttca
    11341 ggaactgggc gtaacaggat tagaatggca ttttattggc ccgttgcagt ctaataaaag
    11401 ccgcctggtg gcagagcatt tcgactggtg tcataccatc gaccgtttgc gcattgctac
    11461 ccgcctcaac gatcagcgcc cggcagaact tccccctctt aacgtactga ttcaaattaa
    11521 tattagtgat gaaaacagta agtccgggat tcaactggca gaactggacg agctggcagc
    11581 ggcggtcgct gaactaccga gtttacgtct gcgcgggctg atggcaatcc ctgcgcctga
    11641 gtcagaatat gtaaggcagt ttgaagttgc acgccaaatg gctgtagcat ttgccggact
    11701 gaaaacgcgc tacccgcata tcgacacgct ctctctggga atgtcggacg atatggaagc
    11761 cgccattgcg gcaggtagca cgatggttcg tatcggcact gcaatttttg gtgcgcgtga
    11821 ttactctaaa aaataaggaa ttaaaggaac gccatgaata cgttgacttt cctgctttca
    11881 acggtcattg agctgtatac catggtgctg ttattacgca tctggatgca gtgggctcat
    11941 tgtgattttt acaacccctt ctcacagttt gtagtgaagg taacgcagcc aattatcggg
    12001 ccactgcgcc gcgttattcc ggcaatgggt cccattgata gcgcctcgct gctggttgcg
    12061 tacattctca gttttatcaa agccatcgtg ctgtttaaag tggtgacctt cctgccaatc
    12121 atctggattg ccggtttact gattctgctg aaaaccatcg gcctgctgat tttctgggtc
    12181 ctgctggtga tggcgattat gagctgggta agccagggtc gtagtccgat tgaatacgtg
    12241 ctgattcagc tggccgatcc gctgctgcgc ccgattcgcc gcctgctacc ggcaatgggt
    12301 gggattgatt tctcgccgat gatcctcgtt ctgctgctgt atgtcatcaa tatgggtgtc
    12361 gcagaagtat tacaggcaac cggaaacatg ctactgccgg ggctgtggat ggcgttatga
    12421 gtgccgtaac agttaatgat gacggtctgg ttttacggct ttatattcag ccgaaagcca
    12481 gccgtgattc tattgtcggt ttacatggcg acgaagttaa agtcgccatt accgcgccgc
    12541 cggttgacgg tcaggccaac agccatctgg tgaagtttct cggtaagcaa ttccgggttg
    12601 ccaaaagcca ggtggtgatt gaaaaaggcg aacttggccg ccacaaacaa attaaaatca
    12661 ttaatccgca acaaatcccg ccagaaatcg cggcgttaat taattaggta tcctatgcaa
    12721 aaagttgtcc tcgcaaccgg caatgccggt aaagtgcgtg agctggcgtc gctgcttagc
    12781 gacttcggtc ttgatatcgt ggcccaaaca gacctcggcg tagattccgc tgaagaaacc
    12841 ggcctgacct ttatcgaaaa cgcgattctg aaagcgcgcc atgcggcaaa agtgaccggt
    12901 ttaccggcaa ttgccgacga ctctggtctg gcggtagatg tacttggcgg cgcgccgggc
    12961 atttactccg cgcgttattc cggtgaagac gcgaccgatc aaaagaatct gcaaaaactg
    13021 ctggaaacac taaaagacgt accggacaac caacgtcagg cgcgtttcca ctgcgtactg
    13081 gtatatctgc gtcacgcgga agatcccact ccgctggtgt gccacggcag ctggccgggc
    13141 atgattactc gtgaaccagc gggtactggc ggctttggtt atgatccaat cttcttcgta
    13201 ccttccgaag ggaaaaccgc tgccgaactg acccgcgaag aaaagagcgc catttcccac
    13261 cgtggtcagg cattgaaact gctgctggac gctttacgta atggttaaat taccgccgct
    13321 aagtctctac attcacatcc cgtggtgcgt gcagaaatgc ccgtactgcg atttcaactc
    13381 tcacgcgttg aaaggagaag tgccgcacga cgattatgtt cagcatctgc ttaacgacct
    13441 ggacaacgat gtggcttacg ctcagggccg tgaagtaaag acaatcttta ttggcggtgg
    13501 tacgccgagc ctgctttccg gcccggcgat gcaaacgctg ctggacggcg tgcgtgcgcg
    13561 tttgccgctg gcagcggatg cagaaattac tatggaagcg aacccaggca cggtagaagc
    13621 ggatcgcttt gtcgattatc agcgtgctgg tgtgaaccgc atctctattg gtgtgcagag
    13681 ttttagcgaa gaaaagctga aacgacttgg gcgtattcat ggcccgcaag aagcgaaacg
    13741 agcggcaaag ctggcgagcg gtttagggtt acgtagcttt aaccttgatt tgatgcatgg
    13801 actaccggat caatcactgg aagaggcgct tggcgatcta cgccaggcca ttgaactgaa
    13861 tccgccgcat ctttcctggt atcaactgac catcgaaccc aatacgctgt ttggttcgcg
    13921 accaccggtg ctgccggacg acgatgcgtt gtgggatatt ttcgaacagg ggcatcagtt
    13981 attaaccgca gcgggttatc agcaatatga aacttccgct tacgccaaac ccggttatca
    14041 gtgccagcac aatctcaact actggcgctt tggcgactac atcggtattg gctgcggcgc
    14101 gcacgggaaa gtgaccttcc cggatgggcg cattctgcgt accaccaaaa cgcgtcatcc
    14161 gcgtggtttt atgcaaggaa ggtatctgga aagccagcgt gatgtcgaag ccgcagataa
    14221 gccgtttgag ttctttatga atcgcttccg tctgctggag gccgcgccgc gcgtggagtt
    14281 tattgcgtat accgggcttt gcgaagatgt gattcgccca cagttagacg aggcgattgc
    14341 ccagggttat ctcacagaat gtgcggatta ctggcagata acggaacatg ggaagctgtt
    14401 tttaaattcg ctgctggagc tttttctggc tgagtaaact tgtattgccg gatgcggcgt
    14461 gaacgcctta tccagcctac atgtggcaac ggttgtaggt ctgataagac gcgcaagcgt
    14521 cgcatcagac gttgattgcc ggatgcgggg tcaacgccgc atcctgctac aaatcgtgca
    14581 ctatatcaaa cttacttaat caccccattg cgccgcagtt gttcggtatc ttttttccag
    14641 cgttcattag cttggttgat atcgtcaatt ttcatcaaaa tcgcgacttt tttactgttt
    14701 atttctgaaa ctttcatcgt taacggatat tgcccttttt cgagcgttac ccagtcatcc
    14761 tgcaatttac ggctgagttt ttgtgattgc agaaaagtct gtcgccgttg ttcgttgata
    14821 tcgaaatctc gtttctgctg aatcggcact tcataacact cttcactctg tttatgacat
    14881 gccgcaatcg ccggttgata aacctctttg tcatatttaa ccgcgaacgc ctcgctatcg
    14941 ttctgtttat taaactcatc acgctgttgg tgaatttttt taaaggcttc ttcgcgggtc
    15001 atttgcttac cgtttgcatc ttttccccaa tatgcatccg ctgctttctg cgccgccacc
    15061 atttgcttat caagcgcttt cacttccttt tccagcgcag gaatcgagtt cttctgggta
    15121 ttcaacgcag attcaagctg actaaagcga taattgaaat cttcgctatt cacgatcaga
    15181 tatttattat tcgttttgat atcgtcgacc acccgcccac gatcgctggc tgccgcctga
    15241 aaagagtaaa agttcaccga ccagccagac gcgggcgttc ctttactggt taacatcgcc
    15301 gagaatttta ccggtttatc tttcgtccag gtctgttcga gcagttcgta atctgccaac
    15361 tgaccgaccc aggtatagag atcgtcactg gaagagacat cgccttccgc tgaccaggtg
    15421 gcctgattac ccttagcatc aagatttttt aaggtgatag catcaagctt gagaatgccg
    15481 tgatactgct ttttgaattg atcttttaag atattttcag ttggcggttc gccatctgcc
    15541 caggcattac cagtcatcaa cataagcagt gccgtgccaa caatccattg ctttttcacc
    15601 cgaaaaacct cacgcggaat aatttattcc ggtgattatc attagggtaa tgaaaattgg
    15661 caatggacgt aagtctgaag tgaaaaagcc ccggcacgat accggggcga ggtgattagt
    15721 actgattgaa gatctgctgg atctgctgcg gatctttggt ttgcgtcaga gccagttgca
    15781 gcagaacgcg cgctttttgc gggttcagcg tgccagaggc gacgaagccg tatttcgcat
    15841 catccacttc ggcatcctga gtggtagcgc ccgtcggtac gcgggaagaa cgcacgactg
    15901 cagtaccgtt tttcgcggcg gtcgccagcg tgtcgaacac agatttatac aggttgccgt
    15961 tacccacacc agcgctaacg atgccatcat agcccgcatc taccagtgct ttagccggaa
    16021 gatcggatgc gttagcgtag ttataaacaa tgccgacttt cggcagttca ttcagcttag
    16081 agacatcgaa tggcgtgtcg ctggtatgct tacgtgccgg ggtacgctgg tagtcaatct
    16141 taccgttgtg aatgtaaccc agaggaccgt agttaacaga cttgaaggtc gctacgtcgg
    16201 tggtgttggt tttggtgacg tcacggccat caagcacggt gtcattcatc actaccagca
    16261 cgccacggtt ggcggaggct ttatcagctg cggtcactac cgcgttatac aggttgaatg
    16321 gaccgtctgc gctcatagac gtggacggac gcattgcgcc gaccatcacc accggtttgt
    16381 cgcatttcac cgtcaggtcg aggaagtaag cagtttcttc catcgtgtcg gtaccgtggg
    16441 taatgacgaa gccgtcggtc ttatcgcagt cggtgttaat ttttttcgcc agtgtcagcc
    16501 agacattatc gttcatgtcc tgggagccga tattcactac ctgctcgcct ttaacgttcg
    16561 caatgtcctt cagttgcggc accgcattaa ccagattttc tacgccaact ttacccgctg
    16621 tgtagttaga tttggttgcg gagtcaccac caccggcaat ggtcccgccg gttgctaaaa
    16681 tggtgatatt gggtaatgcc aatgctgcac cactaaaacc cataaccagt gcggcaagtg
    16741 ccgtcttttt gaaaaactcc atttcattcc tccagttacg tgaacgctac gcattatccc
    16801 ttagctttgt atgggaaatt tgacgttaaa caatttacaa cgtgaatata ttttggagat
    16861 ctacaaagtt agaggcaggt aacaaaacga agaattaaac ggcataaaaa agtattatgc
    16921 cgtcttaaaa tagagaatta ttttaaattc ccgaccaggg ctttgcggct atcttccaga
    16981 gtcacaacac ggctacaaac atctttgcca aactgctgga aatctttttc ctgctttttc
    17041 cactcggttt ggattgagga ttgcagcccc cccaggcttc ccagcacatt ctgtaatggg
    17101 ttaccgccgc ttttcagcac cgctttcgcg cccatttcat taatgctgtc ctgtaaaatt
    17161 ccgcccattg cctgattcac taattgctgg ccttcggcgc gaacctgatc aatggcttta
    17221 tagtgaaacg tcaggccatc gctgcgcgtt tcgataatgc ggttcatctg ctctttcagc
    17281 tgcgcatcaa gtttggtcag acggctgcgc attttgctgc tttcgcccat ctcctgaacg
    17341 ataattttat ccagagcaat acgggctttc tcgacgcggc ttttcgcgcc ttcatcaatc
    17401 cacggcagcg tgctacgtag ttcagcctga taatccttcg cctgctcgcg ctgggcggca
    17461 ttcagggaat attgcttacc gttatacatc acgttgccgt ctggcgtgat caccagattg
    17521 ccgttttcgc ccttcacctg cacggtttgc gggctgacaa tcacatcgtc acgcggcgtg
    17581 acgctgcact ggtagtcggc gtgagcggtc attgccgtca ctgaaagtgc tgccgccagc
    17641 agcattttgc gcatcatagt cttccctcaa gaacaaatca ggccagcatt tgctggcccc
    17701 agattgataa caaagtacgc gttttccatg ccggatgcgg cgtaaacgcc ttatccggcc
    17761 tactcgatcg tgcatctttc caggcatgat aagcgtagcg catcaggcag ttttgcattt
    17821 gtcatcgccc gtatgctttc ttagtcccac caaacgtcga aaagttcgct ggtgcgtacc
    17881 tcatccagtt tgcgctcttc cagccactta cgcacaattg cctgatgttc ttcggtgcat
    17941 ttgccgattt cctgcatgca gatcagacct tcccaggcca gataaccgct gccgtcaaag
    18001 gccagtttgt tcggttcgat aacctcgtta ataaaatcat caacggtttt atcaatctgt
    18061 tcttccgatg taccttccgg gaatcgccat gccaccgaaa atcctaattc ctggaattcg
    18121 tcgatgtgca tttttttacg cagacgacgg ctacggttct ttgccattat ttcaccctct
    18181 cgaacattaa gtcccatact ccgtgaccaa gacgatgacc acgttgttca aatttcgtca
    18241 ctggacgtga tgccggacgc ggtacgtaat cattgctctc tgacaggttt ttataaccgt
    18301 caatagaaga catcacttca agcatatgtt ccgcataagg ttcccagtcg gtcgccatat
    18361 ggaatacgcc ccccagctgc agtttgcttt ttaccagttc ggcaaacggc acctgaacga
    18421 tacggcgttt attatggcgc gctttgtgcc acgggtcagg gaaaaagagc tgcaccatgc
    18481 gcaatgaatt gtcaggaatc attttatgca gcacttcaac cgcatcgtga cacatcacgc
    18541 gcaggttgct taagccctct tcatgcgcag aagccaggca cgcaccaacg cccggtgaat
    18601 gcacttcaat gccgaggaag tcctgctcgg ggcgatcttt agccattgcc accagcgacg
    18661 cccccatgcc aaaaccaatc tcaagcgtca ccggcgcttc acggccaaaa agcgcgggga
    18721 aatccagcat atcttcgctg aactcaacgc ccatcaccgg ccagtagttt tccagcgcat
    18781 gttcctggcc tttggtcagt cgcccctggc ggcgcacaaa actacggata cggcgcagtg
    18841 ggcggccgtt ttcatcaaat tccggtgaaa tgacgtcgtt tttcataaag atttagtcgc
    18901 ttgtgaaagt gttctgaaaa cgggcattat ccaaagttag ttgccggatg caagcatgat
    18961 aaggccgtgg ctgcggaaag ttccggttta caccctgccg tcgctgtgct gcaatcttgc
    19021 ccccaacaac agtgaattcg gtgaccatgc aagcgtcgca attttcagcc caggttctgg
    19081 actggtacga taaatacggg cgaaaaactc tgccctggca aattgacaag acgccctaca
    19141 aagtatggct ctcagaagtg atgttgcaac aaactcaggt tgcgaccgtt atcccctatt
    19201 ttgaacgctt tatggcgcgc ttcccgacgg tgaccgatct cgccaatgcg ccgctcgacg
    19261 aagttctcca cttgtggacc gggcttggct attacgcccg cgcgcgcaat ctgcataaag
    19321 cggcacaaca agtggcgacc ttacacggcg gtaaattccc ggaaaccttt gaggaagttg
    19381 cagcactgcc gggcgtcggg cgttccaccg caggcgcgat tctctcgctt tctctgggta
    19441 agcactttcc gattctcgac ggtaacgtca aacgcgtgct ggcgcgctgc tatgctgtaa
    19501 gcggctggcc tgggaaaaaa gaggtcgaga ataaattatg gagtttaagc gagcaggtga
    19561 cgcccgcggt cggcgtggaa cggtttaatc aggcgatgat ggatttgggc gcgatgattt
    19621 gtacgcgctc gaaaccgaaa tgttcgctct gtccgctaca aaacggatgt attgccgccg
    19681 ccaacaatag ctggtcgctt tatccgggca aaaaaccgaa acagacgctg ccggagcgca
    19741 ccggctactt tttgctatta cagcacgaag atgaagtatt gctggcgcag cgtccgccga
    19801 gcggattgtg gggcggttta tactgtttcc cgcagtttgc cgacgaagaa agtttgcggc
    19861 attggctggc gcaacggcag attgctgccg ataacctgac gcagctgacc gcgtttcgcc
    19921 attccttcag ccatttccac ttagatattg tgcctatgtg gcttcccgtg tcgtcattca
    19981 ccggctgcat ggatgaaggc aatgcgctct ggtataactt agcgcaaccg ccgtcagttg
    20041 gcctggcggc tcccgtggag cgtttgttac agcagttacg cactggcgcg ccggtttagc
    20101 gcgtgagtcg ataaagagga tgatttatga gcagaacgat tttttgtact ttcctgcaac
    20161 gtgaagcaga aggtcaggat tttcagctgt accccggcga gctgggaaaa cgcatctata
    20221 acgagatctc caaagaagcc tgggcgcagt ggcagcacaa gcaaaccatg ctgattaatg
    20281 aaaagaaact caacatgatg aatgccgagc accgcaagct gcttgagcag gagatggtca
    20341 acttcctgtt cgagggtaaa gaggtgcata tcgagggcta tacgccggaa gataaaaaat
    20401 aaaaacagtg ccggagcacg cctccggcaa cttgcataaa aacaaacaca acacgcaccc
    20461 ggaatgatga aaaaatatct cgcgctggct ttgattgcgc cgttgctcat ctcctgttcg
    20521 acgaccaaaa aaggcgatac ctataacgaa gcctgggtca aagataccaa cggttttgat
    20581 attctgatgg ggcaatttgc ccacaatatt gagaacatct ggggattcaa agaggtggtg
    20641 atcgccggtc ctaaggacta cgtgaaatac accgatcaat atcagacccg cagccacatc
    20701 aacttcgatg acggtacgat tactatcgaa actatcgccg ggacagaacc tgccgcgcat
    20761 ttgcgccggg caattatcaa aacgttgctg atgggtgacg atccgagttc ggtcgatctc
    20821 tattccgacg ttgatgatat tacgatttcg aaagaacctt tcctttacgg tcaggtggtg
    20881 gacaacaccg ggcagccgat tcgctgggaa ggtcgcgcaa gcaacttcgc ggattatctg
    20941 ctgaaaaacc gtctgcaaag ccgcaataac gggctgcgta tcatctacag cgtcaccatt
    21001 aacatggtac cgaaccacct tgataaacgt gcgcacaaat atctcggcat ggtccgccag
    21061 gcgtcacgga aatatggcgt tgatgagtcg ctgattctgg caattatgca gaccgaatct
    21121 tcctttaacc cgtatgcggt cagccgttcc gatgcgctgg gattaatgca ggtggtacaa
    21181 catactgccg ggaaagatgt gttccgctcg caggggaaat ccggcacgcc gagccgcagt
    21241 ttcttgtttg atcctgccag caatattgat accggcaccg cgtatctggc gatgctgaac
    21301 aatgtttatc tcggcggaat tgataaccca acatcgcggc gttatgccgt catcaccgcc
    21361 tataacggcg gcgcaggcag cgtgctgcga gtcttttcga atgataagat tcaggctgcc
    21421 aatattatta acaccatgac gccgggcgat gtttatcaga cgctgacgac ccgccatccc
    21481 tctgcggaat ctcgccgtta tctttataaa gtgaataccg cgcaaaaatc ctaccgccgc
    21541 cgataattcc attaaccgcc cctgacgatg ctcaggggca aaaatgttat ctacatcaca
    21601 atttcgtttt gcaaattggg aatgtttgca attatttgcc acaggtaaca aaaaaccagt
    21661 ccgcgaagtt gatagaatcc catcatctcg cacggtcaaa tgtgcttttt caaacactca
    21721 tccgcatcac gatgtgagga aattaacatg aatcttaagc tgcagctgaa aatcctctct
    21781 tttctgcagt tctgtctgtg gggaagttgg ctgacgaccc tcggctccta tatgtttgtt
    21841 accctgaagt ttgacggtgc ttctattggc gcagtttata gctcactggg tatcgccgcg
    21901 gtctttatgc ctgcgctgct ggggattgtg gccgacaaat ggttaagtgc gaaatgggta
    21961 tatgccattt gccacaccat tggcgctatc acgctgttca tggcggcaca ggtcacgaca
    22021 ccggaggcga tgttccttgt gatattgatt aactcgtttg cttatatgcc aacgcttggg
    22081 ttaatcaaca ccatctctta ctatcgcctg caaaatgccg ggatggatat cgttactgac
    22141 ttcccgccaa tccgtatctg gggcaccatc ggctttatca tggcaatgtg ggtggtgagc
    22201 ctgtctggct tcgaattaag ccacatgcag ctgtatattg gcgcagcact ttccgccatt
    22261 ctggttctgt ttaccctgac tctgccgcat attccggttg ctaaacagca agcgaatcag
    22321 agctggacaa ccctgctggg cctcgatgca ttcgcgctgt ttaaaaacaa gcgtatggca
    22381 atcttcttta tcttctcaat gctgctgggc gcggaactgc agattaccaa catgttcggt
    22441 aataccttcc tgcacagctt cgacaaagat ccgatgtttg ccagcagctt tattgtgcag
    22501 catgcgtcaa tcatcatgtc gatttcgcag atctctgaaa ccctgttcat tctgaccatc
    22561 ccgttcttct taagccgcta cggtattaag aacgtaatga tgatcagtat tgtggcgtgg
    22621 atcctgcgtt ttgcgctgtt tgcttacggc gacccgactc cgttcggtac tgtactgctg
    22681 gtactgtcga tgatcgttta cggttgcgca ttcgacttct tcaacatctc tggttcggtg
    22741 tttgtcgaaa aagaagttag cccggcaatt cgcgccagtg cacaagggat gttcctgatg
    22801 atgactaacg gcttcggctg tatcctcggc ggcatcgtga gcggtaaagt tgttgagatg
    22861 tacacccaaa acggcattac cgactggcag accgtatggt tgattttcgc tggttactcc
    22921 gtggttctgg ccttcgcgtt catggcgatg ttcaaatata aacacgttcg tgtcccgaca
    22981 ggcacacaga cggttagcca ctaattacgc aaagaaaaac gggtcgccag aaggtg
//
LOCUS       DAZGVQ010000077        22921 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000077 DAZGVQ010000000
VERSION     DAZGVQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.077"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            29..583
                     /gene="ybeT"
                     /locus_tag="ACHLAG_002880"
     CDS             29..583
                     /gene="ybeT"
                     /locus_tag="ACHLAG_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP0656072.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(593..2020)
                     /gene="djlB"
                     /locus_tag="ACHLAG_002881"
     CDS             complement(593..2020)
                     /gene="djlB"
                     /locus_tag="ACHLAG_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFP0656073.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEDIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALMLLWCRQHRVDYYPLVVMMIEARDLVNDKGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2017..2724)
                     /gene="ybeR"
                     /locus_tag="ACHLAG_002882"
     CDS             complement(2017..2724)
                     /gene="ybeR"
                     /locus_tag="ACHLAG_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP0656074.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRNYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWHSPEDDNLQLLREFLARKEYDDSGNDMFYQLFASDNAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            2888..3865
                     /gene="ybeQ"
                     /locus_tag="ACHLAG_002883"
     CDS             2888..3865
                     /gene="ybeQ"
                     /locus_tag="ACHLAG_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFP0656075.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3935..4417)
                     /gene="ybeL"
                     /locus_tag="ACHLAG_002884"
     CDS             complement(3935..4417)
                     /gene="ybeL"
                     /locus_tag="ACHLAG_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFP0656076.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4652..7234
                     /gene="leuS"
                     /locus_tag="ACHLAG_002885"
     CDS             4652..7234
                     /gene="leuS"
                     /locus_tag="ACHLAG_002885"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFP0656077.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7249..7830
                     /gene="lptE"
                     /locus_tag="ACHLAG_002886"
     CDS             7249..7830
                     /gene="lptE"
                     /locus_tag="ACHLAG_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFP0656078.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7830..8861
                     /gene="holA"
                     /locus_tag="ACHLAG_002887"
     CDS             7830..8861
                     /gene="holA"
                     /locus_tag="ACHLAG_002887"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFP0656079.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWIAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8863..9504
                     /gene="nadD"
                     /locus_tag="ACHLAG_002888"
     CDS             8863..9504
                     /gene="nadD"
                     /locus_tag="ACHLAG_002888"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFP0656080.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9528..10139
                     /gene="cobC"
                     /locus_tag="ACHLAG_002889"
     CDS             9528..10139
                     /gene="cobC"
                     /locus_tag="ACHLAG_002889"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFP0656081.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10399..10716
                     /gene="rsfS"
                     /locus_tag="ACHLAG_002890"
     CDS             10399..10716
                     /gene="rsfS"
                     /locus_tag="ACHLAG_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFP0656082.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10720..11187
                     /gene="rlmH"
                     /locus_tag="ACHLAG_002891"
     CDS             10720..11187
                     /gene="rlmH"
                     /locus_tag="ACHLAG_002891"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFP0656083.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSTLTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11218..13119
                     /gene="mrdA"
                     /locus_tag="ACHLAG_002892"
     CDS             11218..13119
                     /gene="mrdA"
                     /locus_tag="ACHLAG_002892"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFP0656084.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13122..14234
                     /gene="mrdB"
                     /locus_tag="ACHLAG_002893"
                     /gene_synonym="rodA"
     CDS             13122..14234
                     /gene="mrdB"
                     /locus_tag="ACHLAG_002893"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFP0656085.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLAFYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14245..15333
                     /gene="rlpA"
                     /locus_tag="ACHLAG_002894"
     CDS             14245..15333
                     /gene="rlpA"
                     /locus_tag="ACHLAG_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFP0656086.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPIGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15472..16683
                     /gene="dacA"
                     /locus_tag="ACHLAG_002895"
     CDS             15472..16683
                     /gene="dacA"
                     /locus_tag="ACHLAG_002895"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFP0656087.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16794..17057
                     /gene="ybeD"
                     /locus_tag="ACHLAG_002896"
     CDS             16794..17057
                     /gene="ybeD"
                     /locus_tag="ACHLAG_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFP0656088.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17158..17799
                     /gene="lipB"
                     /locus_tag="ACHLAG_002897"
     CDS             17158..17799
                     /gene="lipB"
                     /locus_tag="ACHLAG_002897"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFP0656089.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            18058..19011
                     /gene="ybeF"
                     /locus_tag="ACHLAG_002898"
     CDS             18058..19011
                     /gene="ybeF"
                     /locus_tag="ACHLAG_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFP0656090.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPTSDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19220..20185
                     /gene="lipA"
                     /locus_tag="ACHLAG_002899"
     CDS             19220..20185
                     /gene="lipA"
                     /locus_tag="ACHLAG_002899"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFP0656091.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20286..20489)
                     /gene="tatE"
                     /locus_tag="ACHLAG_002900"
     CDS             complement(20286..20489)
                     /gene="tatE"
                     /locus_tag="ACHLAG_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFP0656092.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20618..21406)
                     /locus_tag="ACHLAG_002901"
     CDS             complement(20618..21406)
                     /locus_tag="ACHLAG_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFP0656093.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21499..21882
                     /gene="crcB"
                     /locus_tag="ACHLAG_002902"
     CDS             21499..21882
                     /gene="crcB"
                     /locus_tag="ACHLAG_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFP0656094.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21936..22145)
                     /gene="cspE"
                     /locus_tag="ACHLAG_002903"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21936..22145)
                     /gene="cspE"
                     /locus_tag="ACHLAG_002903"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP0656095.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22320..22880)
                     /gene="pagP"
                     /locus_tag="ACHLAG_002904"
                     /pseudo
     CDS             complement(22320..22880)
                     /gene="pagP"
                     /locus_tag="ACHLAG_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
BASE COUNT         5890 a         5740 c         5678 g         5613 t
ORIGIN      
        1 aataaaaaga aaatcgaagt attcacatat gaataaaaaa ttaatgtata tattcgcaat
       61 atttatagtt gcagcaatta cctgtattag ccaacccaag aaaacaacgt tgcgtgataa
      121 agccatggtg aattatgcct ttgattattt aagctcacca ggcagtctac cgttaaccac
      181 ggcagccacg gagctttccg cgattcatgg tcactcaaca tcgcaatatc gccttggcga
      241 attttatctt catggtagcg acggtaaacc actggattat acacaggcga gatactggta
      301 tgagcaatca gcagaacagg aaaatccgcg cgcacaaagc aaactggggt ggatctacct
      361 gaaaggcctg ggagtcaaac ccgatacccg taaagcaatt ctctggtata aggaagcagc
      421 tgaacaaggg tatgctcatg ctcaatatac tttgggtttg atctacagaa atggcactgg
      481 tattaatgtt aaccattatg aatctcaaaa atggttaaaa ctggccgcca aacaacatta
      541 caaaaatgcg gaaagattac ttgccggact tcccgcacat taacgtcgac tgtcacccga
      601 agagatggag tattttccct atcaaaccgg caaagataaa aatgtagaaa ataattttga
      661 tgacgcccag acagcccagc ccgccatcgt caacgggcat tggctgcggt tgcggctccg
      721 gctcgacggt ttcctgaccg gtaaaattcg ccgcctgaat taataatcga cgaataatta
      781 atgcttccag tggacaacga tcttctgcat caaggcggct cacaatcggc attaatgatt
      841 cttcggttgg ttggctggcc tgcactaatt gtttggcaac taaatataat ggccataagg
      901 gatgttctgt atcacgcgtg agtgagatcc gcggcttacg tcctttgtgc aaaaccatct
      961 cgaccagtga acgccccaga gcagagagtt tttcatcgct gagtatttcg tataaatgaa
     1021 aacgcgtacg ggcgctgtcg ccggggacat aaagtagcgg ttttccctta tcgttaacca
     1081 gatcacgcgc ttcaatcatc atcaccacta acgggtaata atcgacccga tgctgacggc
     1141 accatagcaa cattaaagca tccgcctggg tggagggata ctcgcgccag tagtcacaaa
     1201 gttctgccag caagctttcg ccttctccac aatagacgcg ttgcgcgtat tcataataac
     1261 gcgcatcttc attatcaggt tcagcttccc gccacgaccg ggcgtaatcc agaagttcgg
     1321 ggatatccag gcgacctgcg ctataccagc ggagtaaatt tctgtgcagt tttgcatcat
     1381 cggggatgat cactgcgcga tgttgcgcta aatacaatgt cagccattct ggatgcgatg
     1441 accaaatccg agcgaacatt tggtaaaaat cgatggtgtc attttgcacg gcaacgggca
     1501 gatgcagaat ggtctggaag ttaaaaacat tccccttcgc aatggcatat aaaaaggatt
     1561 ccagttcttc ttcgtctatt tccccgacgt tttcctcctg caaccaccgc aatctttctg
     1621 ctaataacac cacgcactcg aaggataaat gggcagtgtt catggcgatt gtgcacagcg
     1681 accagcgtaa ttcatcaata tcttccatcg agcaataatt taattgctga atgaatcgct
     1741 gccaggcgga gggcagaaaa cgttcgctat cagaggcaag taacgcgcga aaggcgagca
     1801 gaatttcatg ttctgctacc tcatattctt ctggttgcca aacagattta gcaggcgact
     1861 gcgcaatccg tagcgcctcc tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg
     1921 tttctggatg aaaggacggt aacagcgcca gataagcgcg gcggataata tcgacgtcag
     1981 tcgtttcctc tatttcgagg atcttccagc aatttttcat aggtcgctca tatccttaag
     2041 cgtttccggg catgcagaat agtaagccaa tggttgccag gacaaggtag ggtaatacgc
     2101 attatcactg gcaaataatt gataaaacat atcattgccg gaatcgtcgt attctttacg
     2161 cgctaagaat tcacgtaata actgcagatt atcatcttcg ggagaatgcc aatattgccg
     2221 accaaaggta taaccggcaa agtattgccg ccagttacga taataacgta gagcccgtag
     2281 atgaatgcgc gactggatcc atagactttc ttcttcgctc aaccagttat taagtacgcc
     2341 cattcggctt aaaaaaccca tccggacata atcccaggct ttaattcctc cctcaccaca
     2401 gcattcggcg gtgctggcga caaattgcgc ataagcctga ccttgctcat ttagttcagc
     2461 aagatagtta cgccattcac atggagaata acgaaaccag cggtgataaa accctgccaa
     2521 ccgagcggca tgaccgttct cgatcattcg gtgaattgtg cgcagcaggt catcacggtc
     2581 atcaattccc catgaggaag aaaggtcaaa aaagcatgta cctaaataca ttttgggtga
     2641 tgaatgcgaa ggtaaacagc attcattgcg tatttccatc ggagtggaaa gtgcaaacaa
     2701 tattttttgc gactccatgt ccatataatt tatccttaaa tattcattaa atgaatatta
     2761 gccgcattgc gaggctcgtt tatttatact tttctgacgc gacataatac gcataaaata
     2821 aggcttacgc aaaaatggaa cacataaaaa tataatatgt tttatctgca accataagga
     2881 aattataatg attttcacgt caagttgctg cgataattta tcaatagatg agattatcga
     2941 acgtgctgaa aaaggcgatt gcgaggctca atatattgtt gggttttatt ataatcgcga
     3001 tagcgcaatt gattctccgg acgacgaaaa agccttttac tggctgaagc tggccgccga
     3061 gcaaggtcat tgtgaagcac aatattcctt aggacaaaag tatactgagg ataaaagccg
     3121 tcataaagat aatgagcatg ccatcttttg gctgaaaaaa gctgccctac aaggccatac
     3181 tttcgcttcc aacgcccttg gctggatact ggatcgtgga gaagacccca attataaaga
     3241 agcggttgtc tggtatcaga tagccgcgga gagcggaatg tcttatgcgc aaaataatct
     3301 tgggtggatg tacagaaatg gcaacggagt cgcaaaagac tatgcgctgg catttttctg
     3361 gtacaaacaa gctgcattac aaggccatag tgacgcgcaa aacaatctgg ccgatcttta
     3421 tgaagacgga aaaggcgttg ctcaaaacga gacactcgcc gcattctggt atttgaaaag
     3481 cgcacagcag ggtaatcggc atgctcagtt tcaaattgca tgggattata acgctggcga
     3541 aggggtggac caggactata agcaagcgat gtactggtat ctgaaggctg ccgctcaggg
     3601 gagcgtcggc gcttacgtca acatcggtta tatgtataaa cacggacaag gcgttgagaa
     3661 agattatcag gctgcctttg aatggtttac gaaagccgct gaatgcaatg acgccactgc
     3721 ctggtataac ctggccatta tgtatcacta cggagaagga agacctgtcg atctccgaca
     3781 ggctctcgac ctgtatcgta aagttcagtc atccggaacc agagatgtca gtcaggaaat
     3841 tcgtgagact gaagatttac tgtagagata aaaacaaggg ccggggaatt tcattcggcc
     3901 catattctct ttattgctgt gcgaaatcag actattacgg ctcaaacggg cgtctctgga
     3961 actggtcatg accacatttc gggcatagcg tcagcacttc cggtgtgtag atcgggagat
     4021 ggaagtgaca tttctcgcag accagatttc ccagcccgac cacttctccg ctgtgataaa
     4081 ccccatgatg attgaggtcc tggaaaactt cgcgccattc aagctgcgtt ttatcggtga
     4141 tatctgccag ctcctgccat aagctttctt taatcacccg cataaagacg ctgtcagatt
     4201 cttctttcag gctctcttca tagctcatgg cgaactcttc caggtcacgt cttacagctc
     4261 gcgtcagctc atcgacctcg gttcgcgtta actcccctgt ttttattacg cgctcgcgcg
     4321 cctgttccac cagtgcgtcg atatcacgtt cgccattgcg caggcgttcg ctcagtgacg
     4381 cgaccagttc acggtaatat tgagcaacct tgttcatcgt ttcatctccc gggtcgttaa
     4441 ctgactataa taatagacgt tctttgttcc ttgctttgct aatacgccca caaactctgt
     4501 aaattaatgt atgaataatc cgggcatcat ttcaacgtcg agtgcgcgaa aggctgtttt
     4561 gacgcggacg tttgggctat gctatgcgga tctgaaaaac cacctcaacg ctacatttgt
     4621 agccgtattg aaaacaggac cactggctgc catgcaagag caataccgcc cggaagagat
     4681 agaatccaaa gtacagcttc attgggatga gaagcgcaca tttgaagtaa ccgaagacga
     4741 gagcaaagag aagtattact gcctgtctat gcttccctat ccttctggtc gactacacat
     4801 gggccacgta cgtaactaca ccatcggtga cgtgatcgcc cgctaccagc gtatgctggg
     4861 caaaaacgtc ctgcagccga tcggctggga cgcgtttggt ctgcctgcgg aaggcgcggc
     4921 ggtgaaaaac aacaccgctc cggcaccgtg gacgtacgac aacatcgcgt atatgaaaaa
     4981 ccagctcaaa atgctgggct ttggttatga ctggagccgc gagctggcaa cctgtacgcc
     5041 ggaatactac cgttgggaac agaaattctt caccgagctg tataaaaaag gcctggtata
     5101 taagaagact tctgcggtca actggtgccc gaacgaccag accgtactgg cgaacgaaca
     5161 agttatcgac ggctgctgct ggcgctgcga taccaaagtt gaacgtaaag agatcccgca
     5221 gtggtttatc aaaatcactg cttacgctga cgagctgctc aacgatctgg ataaactgga
     5281 tcactggcca gacaccgtta aaaccatgca gcgtaactgg atcggtcgtt ccgaaggcgt
     5341 ggagatcacc ttcaacgtta acgactatga caacacgctg accgtttaca ctacccgccc
     5401 ggacaccttt atgggttgta cctacctggc ggtagctgcg ggtcatccgc tggcgcagaa
     5461 agcggcggaa aataatcctg aactggcggc ctttattgac gaatgccgta acaccaaagt
     5521 tgccgaagct gaaatggcga cgatggagaa aaaaggcgtc gatactggct ttaaagctgt
     5581 tcacccatta acgggcgaag aaattcccgt ttgggcagca aacttcgtat tgatggagta
     5641 cggcacgggc gcagttatgg cggttccggg tcacgaccag cgcgactacg agtttgcctc
     5701 taaatacggc ctgaacatca aaccagttat cctggctgct gacggctctg agccagatct
     5761 ttctcagcaa gccctgactg aaaaaggcgt gctgttcaac tctggcgaat tcaacggtct
     5821 tgaccatgaa gcggccttca acgccatcgc cgataaactg actgcgatgg gcgttggcga
     5881 gcgtaaagtg aactaccgcc tgcgcgactg gggtgtttct cgtcagcgtt actggggcgc
     5941 gccgattccg atggtgacgc tggaagacgg taccgtaatg ccgaccccgg acgaccagct
     6001 gccggtgatc ctgccggaag atgtggtgat ggacggcatt accagcccga ttaaagcaga
     6061 tccggagtgg gcaaaaacta ccgttaacgg tatgccagcg ctgcgtgaaa ccgacacttt
     6121 cgacaccttt atggagtcct cctggtacta tgcgcgctac acttgcccag agtacaaaga
     6181 aggtatgctg gattccgaag cggctaacta ctggctgccg gtggatatct acattggtgg
     6241 tatcgaacac gccattatgc acctgctcta cttccgcttc ttccacaaac tgatgcgtga
     6301 tgcaggcatg gtgaactctg acgaaccagc gaaacagttg ctgtgtcagg gtatggtgct
     6361 ggcagatgcc ttctactatg ttggcgaaaa cggcgaacgt aactgggttt ccccggttga
     6421 tgctatcgtt gaacgtgacg agaaaggccg tatcgtgaaa gcgaaagatg cggcaggcca
     6481 tgaactggtt tataccggca tgagcaaaat gtccaagtcg aagaacaacg gtatcgaccc
     6541 gcaggtgatg gttgaacgtt acggcgcgga caccgttcgt ctgtttatga tgtttgcttc
     6601 tccggctgat atgactctcg aatggcagga atccggtgtg gaaggggcta accgcttcct
     6661 gaaacgtgtc tggaaactgg tttacgagca cacagcaaaa ggtgatgttg cggcactgaa
     6721 cgttgatgcg ctgactgaag atcagaaagc gctgcgtcgc gatgtgcata aaacgatcgc
     6781 taaagtgacc gatgatatcg gccgtcgtca gaccttcaac accgcaattg cggcgattat
     6841 ggagctgatg aacaaactgg cgaaagcacc aaccgatggc gagcaggatc gcgctctgat
     6901 gcaggaagcg ctgctggccg ttgtccgtat gcttaacccg ttcaccccgc acatctgctt
     6961 cacgctgtgg caggaactga aaggcgaagg cgatatcgac aacgcgccgt ggccggttgc
     7021 tgacgaaaaa gcgatggtgg aagactccac gctggtcgtg gtgcaggtta acggtaaagt
     7081 ccgtgccaaa atcaccgttc cggtggacgc aacggaagaa caggttcgcg aacgtgctgg
     7141 ccaggaacat ctggtagcaa aatatcttga tggcgttacc gtgcgtaaag tgatttacgt
     7201 accaggtaaa ctcctcaatc tggtcgttgg ctaagcgcgg gaggaagcgt gcgatatctg
     7261 gcaacattgt tgttatctct ggcggtgtta atcaccgccg ggtgtggctg gcatctgcgt
     7321 gataccacgc aggttccttc cactatgaag gtcatgatcc tggactcagg cgatccgaac
     7381 gggccattaa gccgtgcggt gcgtaaccag ttacgtctga atggtgtcga gttgcttgat
     7441 aaagaaacca cgcgtaagga cgttccatcc ctgcgtttgg gtaaagtgag catcgcgaaa
     7501 gataccgcat cggtattccg taacggtcaa acagcagagt atcagatgat catgacggtg
     7561 aatgcgaccg tgttgatccc cggccgtgat atctacccga ttagtgccaa agtcttccgt
     7621 tcgttctttg ataacccgca aatggcgtta gcgaaagata acgaacaaga catgatcgta
     7681 aaagagatgt acgaccgtgc tgccgaacag ctgattcgta agctgccaag catccgtgct
     7741 gcggatattc gttccgacga agaacagacg tcgaccacaa cggatactcc ggcaacgcct
     7801 gcacgcgtct ccaccacgct gggtaactga tgattcggtt gtacccggaa caactccgcg
     7861 cgcagctcaa tgaagggctg cgcgcggcgt atcttttact tggtaacgat cctctgttat
     7921 tgcaggaaag ccaggacgct gttcgtcagg tagctgcggc acaaggattc gaagaacacc
     7981 acactttttc cattgatccc aacactgact ggaatgcgat cttttcgtta tgccaggcta
     8041 tgagtctgtt tgccagtcga caaacgctat tgctgttgtt accagaaaac ggaccgaatg
     8101 cggcgatcaa tgaacaactt ctcacactca ccggacttct gcatgacgac ctgctgttga
     8161 tcgtccgcgg taataaatta agcaaagcgc aagaaaatgc cgcctggttt actgctcttg
     8221 cgaatcgcag cgtgcaggtg acctgtcaga caccggagca ggctcagctt ccccgctgga
     8281 ttgctgcgcg cgcaaaacag ctcaacttag aactggatga cgctgcaaat caggtgctct
     8341 gctactgtta tgaaggtaac ctgctggcgc tggctcaggc actggagcgt ttatcgctgc
     8401 tctggccaga cggcaaattg acattaccgc gcgttgaaca ggcggtgaat gatgccgcgc
     8461 atttcacccc ttttcattgg gttgatgctt tgttgatggg aaaaagtaag cgcgcgttgc
     8521 atattcttca gcaactgcgt ctggaaggca gcgaaccggt tattttgttg cgcacattac
     8581 aacgtgaact gttgttactg gttaacctga aacgccagtc tgcccatacg ccactgcgtg
     8641 cgttgtttga taagcatcgg gtatggcaga accgccgggg catgatgggc gaggcgttaa
     8701 atcgcttaag tcagacgcag ttacgtcagg ccgtgcaact cctgacacga acggaactca
     8761 ccctcaaaca agattacggt cagtcagtgt gggcagagct ggaagggtta tctcttctgt
     8821 tgtgccataa acccctggcg gacgtattta tcgacggttg atatgaaatc tttacaggct
     8881 ctgtttggcg gcacctttga tccggtgcac tatggtcatc ttaaacccgt ggaaacgctg
     8941 gcgaatttga ttggtctgac gcgggtcaca atcatcccta ataatgttcc tccgcatcgt
     9001 ccccagccgg aagcgaacag cgtgcagcgt aaacacatgc ttgaactggc gattgccgac
     9061 aagccattat ttactcttga tgaacgcgag ctaaagcgca atgccccctc ttacactgcg
     9121 caaacactga aagagtggcg gcaggaacaa gggccggacg tgccgctggc gtttattatt
     9181 ggtcaggatt cattgctgac ctttccgacc tggtacgaat acgaaacgat actcgacaat
     9241 gcacatttga tcgtctgtcg gcgtccaggt tacccacttg aaatggcgca accgcaatac
     9301 cagcaatggc tggaagatca tttgacacat aacccggaag atcttcacct tcagcctgcc
     9361 ggtaaaattt atctggctga aacgccgtgg tttaacatct cggcgaccat catccgcgaa
     9421 cgtttgcaaa acggtgaatc gtgtgaggat ttattgccgg aaccggtact gacttacatt
     9481 aaccaacaag gcttgtatcg ctgatacctg tcgttacgga gggcatgatg cgactgtggt
     9541 taattcgtca tggtgaaacg caagcgaata tcgatggtct atacagcggt catgcgccca
     9601 cccccctgac cgcgcgcggt attgagcaag cgcaaaatct gcatacgctg ctacatggtg
     9661 tttcctttga tctggtttta tgcagtgaac tggaacgggc acagcatacc gcgcgactgg
     9721 ttctcagtga ccgccagctc cccgtgcaaa tcatccctga actcaacgaa atgttttttg
     9781 gcgactggga gatgcgacat catcgcgacc tcatgcaaga agatgccgaa aactatagcg
     9841 cgtggtgcaa tgactggcag catgcaatcc ccacgaacgg tgaaggattt caggcatttt
     9901 cgcaacgtgt ggaacgcttt atcgcaaggc ttagtgaatt tcagcactat cagaatattt
     9961 tagtcgtcag ccatcagggt gtactgagtc tgttaatcgc ccgtttaatt ggcatgcctg
    10021 ccgaggctat gtggcatttt cgcgttgacc aggggtgttg gagcgccatt gatatcaacc
    10081 aaaaattcgc gacgctacgc gtcctcaata gccgtgccat cggggtcgag aatgcatgac
    10141 ttttctgttt ttttacgggt aagccgcaac gaccattgac agacccgggc aggctgatat
    10201 tctccgcagc cagacttttt tcgccagaca cgactttgta gaaattgttt tacaaaaatg
    10261 gcgatgcaat ctgcggcgcg gggtgggatg atagcccact ttcgaaagcc gattcggcga
    10321 caattgtccc gaaatcgcct ctggttcagg tatactgaca gaccattttt atctatttga
    10381 ttcacccagg gggaaaactt gcagggtaaa gcactccagg attttgttat cgacaaaatt
    10441 gatgacctca aaggtcagga catcatcgcc ttagacgttc aaggcaaatc cagcatcacc
    10501 gactgcatga tcatctgtac gggtacgtcc agccgtcatg ttatgtccat tgctgaccac
    10561 gttgtgcagg agtctcgcgc agcgggcctg ttaccgctcg gcgtagaagg tgaaaacagc
    10621 gccgactgga ttgtcgtgga tttgggcgat gtgattgtcc atgtcatgca ggaagagagc
    10681 cgtcgcctgt atgaactgga aaaactctgg agttaatgcg tgaagctgca acttgtcgcc
    10741 gtgggaacga aaatgccgga ctgggtacaa accggtttta ccgagtacct gcgtcgtttt
    10801 ccgaaagata tgcccttcga gctgattgaa attccggccg gaaaacgcgg caagaatgcg
    10861 gacatcaagc gcatactcga caaagagggt gagcagatgt tggcggccgc aggcaaaaac
    10921 cgcattgtca ccctcgatat tccaggcaag ccctgggata cgccgcagtt agccgctgag
    10981 ctggaacgct ggaagctgga tggtcgcgac gtcagtctac tgattggcgg gcctgaaggg
    11041 ttgtcgcctg cctgtaaagc ggcggcggag cagagctggt cgctgtcgac gcttaccctc
    11101 ccccatccgc tggttcgcgt gctggtcgca gagagtctgt accgggcgtg gagcatcacc
    11161 accaaccatc cttatcaccg tgagtgataa gggagctttg agtagaaaac gcagcggatg
    11221 aaactacaga actcttttcg cgactatacg gcagagtccg cgctgtttgt gcgccgggcg
    11281 ctggtcgcct ttttggggat tttgctgctg accggcgtgc ttatcgccaa cctgtataat
    11341 ctgcaaattg ttcgctttac cgattaccag acccgctcta atgaaaaccg cattaagctg
    11401 gtgcctatcg cgcccagccg cggcattatc tacgaccgta acggtatccc tctggcactc
    11461 aaccgcacta tctaccagat agaaatgatg ccggagaaag tcgataacgt gcagcaaacg
    11521 ctggacgctt tgcgcagcgt ggttgatctg accgatgacg atattgctgc attccgaaaa
    11581 gagcgcgcac gttcacaccg tttcacctct attcccgtga aaactaacct gaccgaagta
    11641 caagtagctc gctttgccgt caatcagtac cgttttccgg gtgtcgaagt taaaggctat
    11701 aaacgtcgtt actatcctta cggttcggcg ttgacccacg tcatcggcta tgtgtcgaaa
    11761 atcaacgata aagacgtcga acgcctgaat aatgacggca aactggccaa ctatgcggca
    11821 acgcatgata tcggtaagct gggcattgag cgttactatg aagacgtgct gcacggtcag
    11881 accggttatg aagaggttga agttaacaac cgtgggcgtg ttatccgcca gttaaaagaa
    11941 gtaccaccgc aagccggaca cgatatttac ctgacgctgg atctcaaact ccagcaatat
    12001 attgaaacgc tgctggcggg tagccgcgca gctgtggtag tcaccgatcc gcgtacaggt
    12061 ggggtgctgg cactggtttc cacgcctagt tatgacccaa acctgtttgt tgacggtatc
    12121 tccagcaaag attattccgc cttgctgaac gacccgaata caccgctggt gaaccgcgcc
    12181 acacaggggg tttatcctcc tgcgtctaca gttaaaccct atgtggcggt ttcggcatta
    12241 agcgccgggg tgatcacgcg caatacgacg ctgtttgacc caggctggtg gcaactacca
    12301 gggtcggaaa aacgttatcg tgactggaaa aaatggggcc acgggcgtct gaatgtcaca
    12361 agatcgctgg aagaatctgc ggataccttc ttctatcagg tggcctacga tatggggatc
    12421 gatcgcctct ccgaatggat gggtaaattc ggttatggtc attacaccgg tatcgacctg
    12481 gcggaagaac gttccggcaa catgcctacc cgcgaatgga aacagaaacg ctttaaaaaa
    12541 ccgtggtatc agggtgacac cattccggtt ggtatcggcc agggttactg gacagcgacc
    12601 ccaatccaga tgagtaaggc actgatgatc ctgattaatg acggtatcgt gaaggttccg
    12661 catttgctga tgagcaccgc cgaagacggc aaacaggtgc catgggtaca accgcatgaa
    12721 ccgcccgtcg gcgatattca ttccggttac tgggagctgg cgaaagacgg tatgtacggt
    12781 gttgctaacc gccctaacgg tacggcgcat aaatactttg ccagcgcacc gtacaaaatt
    12841 gcggcgaaat ctggtaccgc tcaggtcttc ggtctgaaag cgaacgaaac ctataatgcg
    12901 cacaaaattg ccgagcgttt acgtgaccac aaactgatga ccgcctttgc gccatacaac
    12961 aatccgcaag tggctgtcgc catgattctg gagaacggcg gcgcgggtcc ggcggttggt
    13021 acgctgatgc gccagatcct cgaccacatt atgctgggtg ataacaatac cgatctgcct
    13081 gcggaaaatc ctgcggttgc cgcagcggag gaccattaat catgacggat aatccgaata
    13141 aaaaaacatt ctgggataaa gtccatctcg atcccacaat gctgctgatc ttactggcat
    13201 tgctggttta cagcgccctg gttatctgga gcgccagcgg tcaggatatt ggcatgatgg
    13261 agcgtaaaat aggccaaatc gcgatgggtc tggtcatcat ggtggtgatg gcgcaaattc
    13321 ctccacgcgt ttatgaaggc tgggccccct atctctatat catctgtatt attttgctgg
    13381 tggcggtaga tgctttcggt gccatctcta aaggcgctca acgctggctg gacctcggta
    13441 ttgttcgttt tcagccgtcg gaaattgcca aaatagccgt accactgatg gttgcgcgct
    13501 ttatcaaccg cgacgtttgc ccgccatcgt tgaagaacac tggcatcgcg ctggtgctga
    13561 tatttatgcc cacgctgctg gtggctgcac agcctgacct gggaacatca atcctcgttg
    13621 cgctttccgg tctgtttgta ctgttcctct ctggccttag ctggcgtctg attggcgtcg
    13681 cagtagtgct ggtggcggcg ttcattccta ttctgtggtt cttcctgatg catgattacc
    13741 agcgccagcg cgtaatgatg ctcctggacc cggaatcaga cccactcggc gcgggctatc
    13801 acattattca gtctaaaatt gctattggct ccggcggatt acgcggcaaa ggctggctgc
    13861 atggcactca gtcacaactt gaatttctac ccgaacgcca taccgacttt atcttcgcgg
    13921 tactggcgga agagctggga ttagtgggca ttctgattct gctcgctttc tacattctgc
    13981 tgatcatgcg cgggctgtgg atagccgcca gagcgcaaac cacctttggt cgcgtcatgg
    14041 ctggtggctt aatgctgata ttattcgttt atgtcttcgt aaatattggt atggtaagcg
    14101 gtattctgcc ggttgtaggg gttccgctcc cactggtcag ttatggagga tcggcgctaa
    14161 ttgtgctgat ggctgggttc gggattgtaa tgtcaatcca cacccacagg aaaatgttgt
    14221 cgaaaagcgt gtaagaggtg cgcaatgcgt aagcagtggc tcgggatctg catcgcggca
    14281 ggaatgctcg cggcatgtac aagcgatgat ggtcagcaac agacggtaag tgtaccgcag
    14341 cctgcggtat gtaacggccc catagttgaa attagcgggg cggacccgcg tttcgaacca
    14401 ctgaacgcga cggcaaatca ggattaccag cgcgacggta aaagctacaa aatcgtgcag
    14461 gatccgtctc gatttagcca ggcgggactg gcggcaatct atgatgccga accaggcagt
    14521 aacctgacgg cctctggcga agctttcgat ccgacacagc tgacggcggc ccatccaacg
    14581 cttccgatcc ccagctacgc cagaatcact aacctggcta acgggcgaat gatcgtggtg
    14641 cgcattaatg atcgcggccc ttacggcaac gaccgcgtta tttcgctttc tcgcgcggca
    14701 gctgaccgtc ttaacacgtc aaacaacacc aaagttcgta tcgatccgat tattgtcgcc
    14761 caggatggtt cgctttctgg tcctggtatg gcttgtacca cagtcgccaa acagacttac
    14821 gccctgcctg cacctcccga tttaagcggt ggcgcgggaa caagttcagt gtctggcccg
    14881 cagggtgaca ttcttccggt cagtaattcg acgctaaaaa gcgaagatcc gatcggcgcg
    14941 ccggtaacca gtagcggttt cctcggcgct ccaacgacct tagcgcctgg tgtactggaa
    15001 ggcagcgaac cgacgcctgc gccacagccc gttgttacag ctccgtcgac aacgcctgca
    15061 acctcgcctg caatggtgac accgcaagcc gcctcgcaaa gcgccagtgg caactttatg
    15121 gtgcaagtcg gggccgtaag cgatcaggct cgtgcgcaac agtaccaaca gcaactggga
    15181 cagaagttcg gcgtccccgg tcgcgtaact caaaatggcg cggtctggcg gatccagctt
    15241 ggcccattcg ccagcaaagc cgaagccagt accttgcagc aacgtttgca aaccgaagcc
    15301 caattacagt catttattac taccgcgcag tagcgtaaag caggcatctg atgtgtcaat
    15361 ttctgtaaat gacgttaaca aagtcacgcg gaaagtcaga tgcctgccgg tagtgcattt
    15421 gctatagtag ggcacttttt taattccatc acggatgtcg tagttcagac catgaatacc
    15481 attttttccg ctcgtatcat gaagcgcctg gcgctcacca cggctctttg cacagccttt
    15541 atctctgctg cacatgccga tgacctgaat atcaaaacta tgatcccggg tgtaccgcag
    15601 atcgatgcgg agtcctacat cctgattgac tataactccg gcaaagtgct cgccgaacag
    15661 aacgcagatg tccgccgcga tcctgccagc ctgaccaaaa tgatgaccag ttacgttatc
    15721 ggccaggcaa tgaaagccgg taaatttaaa gaaactgatt tagtcactat cggcaacgac
    15781 gcatgggcca ccggtaaccc ggtgtttaaa ggttcttcgc tgatgttcct caaaccgggc
    15841 atgcaggttc cggtttctca gctgatccgc ggtattaacc tgcaatcggg taacgatgct
    15901 tgtgtcgcca tggccgattt tgccgctggt agccaggacg cttttgttgg cttgatgaac
    15961 agctacgtta acgcactggg cctgaaaaat acccacttcc agacggtaca tggtctggat
    16021 gctgatggtc agtacagctc cgcgcgagat atggcgctga tcggccaggc attgatccgt
    16081 gacgtaccga atgaatactc gatctataaa gaaaaagaat ttacgtttaa cggtattcgc
    16141 cagctgaacc gtaacggcct gttatgggat aacagcctga atgtcgacgg catcaaaacc
    16201 ggacacactg acaaagcagg ttacaacctt gttgcttctg cgactgaagg ccagatgcgc
    16261 ttgatctctg cggtgatggg cggacgtact tttaaaggcc gtgaagccga aagtaaaaaa
    16321 ctgctaacct ggggcttccg tttcttcgaa accgttaacc cactgaaagt aggtaaagag
    16381 ttcgcctctg aaccggtttg gtttggtgat tctgatcgcg cttcgttagg tgttgataaa
    16441 gacgtgtacc tgaccattcc acgtggtcgc atgaaagatc tgaaagccag ctatgtgctg
    16501 aacagcagtg aattgcatgc gccgctgcaa aagaatcagg tcgtcggaac tatcaacttc
    16561 cagcttgatg gcaaaacgat cgagcaacgc ccgctggttg tgttgcaaga aatcccggaa
    16621 ggtaacttct tcggcaaaat cattgattac attaaattaa tgttccatca ctggtttggt
    16681 taaaaattga acacttgaaa gtgtaatttc cgtccccata tactaagcat cagtaaaaaa
    16741 actcccgcct tctggcggga gttgctattt aattacgtta cgccggagct gacatgaaaa
    16801 ccaaacttaa cgaactgctt gaattcccta ctccttttac ttacaaagtt atggggcagg
    16861 cgttacctga gctggttgat caggtggttg aagtggtaca gcgccatgcg ccaggtgact
    16921 acaccccaac ggtaaaacca agcagcaaag gcaactacca ctcggtatct atcactatca
    16981 acgccactca tatcgagcag gttgaaacac tgtatgaaga actgggcaaa atcgatattg
    17041 tccgcatggt tctgtaactc gcttctccgt tacccggctc ctggtcgggt aactcccctc
    17101 tcctcgctgt gatatacttt tcccccactt ttactcattc tccacggaga tgccgttttg
    17161 tatcaggata aaattcttgt ccgccagctc ggtcttcagc cttacgagcc aatctcccag
    17221 gctatgcatg aattcaccga tacccgcgat gatagtaccc ttgatgaaat ctggctggtc
    17281 gagcactatc cggtattcac ccaaggtcag gcaggaaaag cggagcacat tttaatgccg
    17341 ggtgatattc cggtgatcca gagcgatcgc ggtgggcagg tgacttatca cgggccggga
    17401 caacaggtga tgtatgtgtt gcttaacctg aaacgccgta aactcggtgt gcgtgaactg
    17461 gtgaccttgc ttgagcaaac agtggtgaat accctggctg aactgggtat agaagcgcat
    17521 cctcgggctg acgcgcctgg tgtctatgtc ggggaaaaga aaatttgctc actgggttta
    17581 cgaattcgac gcggttgttc attccacggt ctggcattaa acgtcaatat ggatctttca
    17641 ccatttttac gtattaatcc ttgtgggtat gccggaatgg aaatggctaa aatatcacaa
    17701 tggaaacccg aagcgacgac taataatatt gctccacgtt tactggaaaa tattttagcg
    17761 ctactaaaca atccggactt cgaatatatt accgcttaat tccatacatc aatggcccaa
    17821 tttacactgg gtcattactc ataatacctg ctaatgcttt acatctcctt cgcgtttgta
    17881 tattttcttt atcacttgca gaaatgcggt gccacgcctt gctccgtaac tcaccccatt
    17941 gattaaccta ttgaaatagt tgattatggt gagcaaaagc gttccgttcc aattagacat
    18001 aagattattg taatttttta aatattattt ttccatgaat aaaattggag taagtgcgtg
    18061 gatagtaata atcaaattga accctgttta agcagaaagt cgtcagaagg taaaccacaa
    18121 atatttacaa ccttacgaaa tattgatctt aaccttctga ctatttttga agctgtatat
    18181 gtacataaag ggatcgttaa tgcagcgaaa gtgcttaatc tgaccccctc ggcaatcagt
    18241 cagtctattc agaaactgcg cgttatattc cctgacccat tgtttattcg caaaggccag
    18301 ggtgtcactc ctaccgcatt tgcgatgcat ctacatgagt atatcagtca gggccttgag
    18361 tccattcttg gcgcgctgga tatcgaagga agctatgata agcaacgaac gataactatt
    18421 gcgaccactc cctcggtcgg agccctggtc cttcctgtca tctatcgggc gattaaaact
    18481 cactatccgc agcttttact gcgcaacccg cccaccagcg acgcggaaaa ccaactcagt
    18541 cagtttcaaa ccgatctcat catcgataac atgttttgca ccaatcgtac ggtgcaacat
    18601 catgttctgt tcaccgacaa tatggtgtta atttgccgcg agggaaatcc actactctct
    18661 ttagaagatg acagagagac tatcgacaac gctgcgcatg tacttctgtt accggaagag
    18721 caaaatttca gcggtctgcg gcagagagtt caagaaatgt ttccggaccg gcaaatcaat
    18781 ttcaccagct acaacatttt gacaatcgct gcactggttg ccaacagtga catgttagcg
    18841 attattccca gccgttttta taacctgttt agccgctgct ggccgctgga aaaactgcct
    18901 tttccgtcct taaatgagga gcaaatagat ttctctatcc actacaacaa attcagcctg
    18961 cgtgatccca tcctgcacgg agtaatagat gttatccgta atgcattttg aaaaagtatt
    19021 gttacagcca gatcacagcg cagaacgata aaatggcaca atccaacaac aattttacat
    19081 tttcgcgacc gctttggctg ctttcaggtc cgtttcaatg atatactgcc agtcgttaat
    19141 tcaaaaatag ttgataatta caacaatcta ttgaattgaa acgctttcct tcgtaattcg
    19201 caactggaac acgcacgcta tgagtaaacc cattgtgatg gaacgcggtg ttaaataccg
    19261 cgatgccgat aagatggccc ttatcccggt taaaaacgtg gcaacagagc gcgaagccct
    19321 gctgcgcaag ccggaatgga tgaaaatcaa gcttccagcg gactctacac gtatccaggg
    19381 catcaaagcc gcaatgcgca aaaatggcct gcattctgtc tgcgaggaag cctcctgccc
    19441 taacctggcg gaatgcttca accacggcac agcaacgttt atgatcctcg gcgctatttg
    19501 tacccgccgt tgtccgttct gtgatgttgc ccacggtcgc ccggtagctc ctgatgccaa
    19561 tgaaccagtg aaactggcgc agaccattgc cgatatggcg ctgcgttatg tggttatcac
    19621 ctccgttgac cgtgatgacc tgcgcgatgg cggtgcccag cactttgcgg attgcattac
    19681 tgccattcgg gaaaaaagcc cgcaaatcaa aattgaaact ctggtgccgg atttccgtgg
    19741 tcgtatggat cgtgctctgg atattctgac tgcaacgcca ccagatgtgt tcaaccataa
    19801 cctggaaaac gtaccgcgta tttaccgtca ggtacggcct ggtgcagatt acaactggtc
    19861 gctgaagctg ctggaacgct ttaaagaagc gcatccggaa atcccgacca agtctggtct
    19921 gatggtggga ctgggtgaaa ccaatgaaga aattattgag gtaatgcgcg acctgcgccg
    19981 tcatggtgtg acgatgttaa cgctggggca atatttgcag ccaagccgcc atcacctgcc
    20041 ggttcaacgt tacgttagcc cggatgagtt cgacgaaatg aaagccgaag cgctggcgat
    20101 gggctttacc catgctgcat gcggtccgtt tgtccgctct tcttaccacg ccgatttgca
    20161 ggcgaaaggg atggaagtta agtaatcctg taataattac ttacatctgt ctgataaaaa
    20221 acccgctttt tgaagcgggt tttttgtatc gaacaagata ttgagggagc gtcctgctcg
    20281 ccacgtcact ctttatgaga gagcttttca gcctgaagat caacgtctgc gccttttttc
    20341 gccgcagcat cgtcatcatt catcgccttc ttgaaccctt taatggccgc tccaaggtct
    20401 ccgcccagcg tacgtaactt cttagtccca aacagcagaa cgaccagcgc cgcaactacc
    20461 agcagtttgg taatactaat ctcacccata gataccttct tgacatataa ccggcaacag
    20521 aacgcgcctt aaaaaccaga gaatattaac ggtcattcga cgcgcgcaca caatagcaat
    20581 ctgtaacaag gtgaatcaag cgccgagtaa aaaaacatca taataattgc ggcggcgcaa
    20641 agcgacggtt gtttaagacg ggcagttgcg cgcgcacctg acgcacacgt tcgggcgtca
    20701 cttccgccat aatgagtgca ggcatttctg acgctgccgc aatggtgacg ccaaagggat
    20761 ctataatccg gctttgaccg atatttttgt tcccgcactc ccccgccgcc accatataac
    20821 aggtggtatc cagcgcacga gcggcaagca acgttgacca atgatgctct ttgagcggcc
    20881 cgcgaaccca ggcggcagga agtaccagga tttcagctcc ctgtaatgcc tgtgccagcg
    20941 ccagctctgg aaagcgtaag tcataacagg tcatcagacc gaccttcatc ccctccacct
    21001 cgagtaacgg agcgatttca ttaccagcat caacacggcg tgattcctga atggcaaatg
    21061 catcatagag atgcaatttg gcataacggg cgacgatgtt tcctgcctgt agcgccacca
    21121 gcatattcca tgcgcgcccc ggcgttgaag gaacatgaat cgtcagaatt gtcgtcatca
    21181 tgttacgttt actttctcgc cgtaaacgtc cgaggaattc gccttccagc agctgtgctg
    21241 atttaaccga tagatctgca tcatgatcat cgcgcgccag caatgcttcc ggcaggacga
    21301 acagcgatac gtcgttttcc gccgcctgcg ccatcaacga ggcacaaatc tcagcgttct
    21361 tttcccacac agatgtaaca gcaaactgtc ctgcggcaac cagcattgcc acctcctgtc
    21421 agagtcgtaa aattcgccaa cgacgattat tcggcgttac acttatcact catacaaatc
    21481 aaatagcagg attttgcagt gttacaactt cttttagcag tttttattgg cggtggtacg
    21541 ggaagcgtgg cgagatggct gttaagtatg cgatttaacc cgctgcatca ggcgattccg
    21601 ttggggacgc tggcagcaaa cctgattggg gcattcatca taggaatggg attcgcctgg
    21661 ttcagcagga tgacgaacat tgatccagtg tggaaagtat taatcaccac cggattttgt
    21721 ggcggtctaa caaccttctc aacattttcg gcagaagtgg tgtttttgtt acaagagggc
    21781 cgctttggct gggcgttact gaacgttttc gtcaaccttc tggggtcttt tgccatgaca
    21841 gcactggctt tctggctgtt ttcggcctca accgcacact aaagaccaaa aaaaacccgc
    21901 tgattaagcg ggttttgaat tcttgctgac gtatcttaca gagcgattac gtttgcagca
    21961 gaagggcctt tggcaccgtt agtgatttcg aactctacgc gctgaccttc agcaagagtt
    22021 ttaaaaccat tagtctggat tgcagagaag tgtacgaaca cgtctttgct gccgtcttcc
    22081 ggagtaatga aaccgaatcc tttggactca ttaaaccact taacgttacc tttaatctta
    22141 gacatcaaaa ttacctttac atgaaaaata gacacaaatg ctgtgtcggt taccagtaca
    22201 ccaattgtgg tacgctttgt ccagtgcaac tttactaaaa agtgataaaa gtcgcgttat
    22261 ttttgatgtt acttatttta gctattgatt tttaagaagt tactaaaact tcatttgtct
    22321 caaaactgaa agcgcatcca ggcaaagtac acattgccat tgttgtaggt acccggaatg
    22381 taggtcatct gaaaagtcac tgggccataa cccacggagg ccaatggcag tagaaccggg
    22441 agagggatgt aattccagtt atcgcgtgcc gttacgccag cggtgaatcc cagacctaaa
    22501 tgaaaatttt catccgccag cggtcgccag gtactttccc atccgtatcc ggcaatcggt
    22561 tcccatttgt tccacgagtc cttaaatgcc atggcataca ggccatgcca gtttcctttt
    22621 tcatcccaac gcgacaggcc aaaaccgcca ccccacggtc gctcgttata gcgatcggtt
    22681 ttttctttgt cgtaagcgaa acgtgcatgc caggtgatgg caggaatata taaatcataa
    22741 tgttcaggct gttgccaggt ttgtgcaata ttttatctaa atgttgtcat ccactcatct
    22801 gcgttagcaa aaactttacc aacgctgatt aactgaataa aaacaaagga aaagatagcg
    22861 acatatttac tcacgttcat ttgtgaccat aaaacattta tcaaaaatct actactagca
    22921 t
//
LOCUS       DAZGVQ010000078        22590 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000078 DAZGVQ010000000
VERSION     DAZGVQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.079"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            84..3818
                     /gene="yfaL"
                     /locus_tag="ACHLAG_002905"
     CDS             84..3818
                     /gene="yfaL"
                     /locus_tag="ACHLAG_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFP0656096.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANRARVS
                     QSQGDDIQMRLGLHSEWRTAVHVIPTLDLNYYHDPHSTEIEEDGSTISDDAVKQRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQMAGFLSMTVKW"
     gene            complement(3946..4668)
                     /gene="ubiG"
                     /locus_tag="ACHLAG_002906"
     CDS             complement(3946..4668)
                     /gene="ubiG"
                     /locus_tag="ACHLAG_002906"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFP0656097.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            4815..7442
                     /gene="gyrA"
                     /locus_tag="ACHLAG_002907"
     CDS             4815..7442
                     /gene="gyrA"
                     /locus_tag="ACHLAG_002907"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFP0656098.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7591..8817
                     /locus_tag="ACHLAG_002908"
     CDS             7591..8817
                     /locus_tag="ACHLAG_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 family protein"
                     /protein_id="HFP0656099.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERTAGKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMSNDGWHSFLALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLTGALDGAAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPAGAFFVSL"
     gene            8998..11442
                     /gene="dmsA"
                     /locus_tag="ACHLAG_002909"
     CDS             8998..11442
                     /gene="dmsA"
                     /locus_tag="ACHLAG_002909"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFP0656100.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCSASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            11453..12070
                     /gene="dmsB"
                     /locus_tag="ACHLAG_002910"
     CDS             11453..12070
                     /gene="dmsB"
                     /locus_tag="ACHLAG_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFP0656101.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCSSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            12072..12935
                     /gene="dmsC"
                     /locus_tag="ACHLAG_002911"
     CDS             12072..12935
                     /gene="dmsC"
                     /locus_tag="ACHLAG_002911"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFP0656102.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(12971..13597)
                     /gene="ycaC"
                     /locus_tag="ACHLAG_002912"
     CDS             complement(12971..13597)
                     /gene="ycaC"
                     /locus_tag="ACHLAG_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFP0656103.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            13912..15060
                     /gene="ycaD"
                     /locus_tag="ACHLAG_002913"
     CDS             13912..15060
                     /gene="ycaD"
                     /locus_tag="ACHLAG_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0656104.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            15270..16700
                     /gene="ycaM"
                     /locus_tag="ACHLAG_002914"
     CDS             15270..16700
                     /gene="ycaM"
                     /locus_tag="ACHLAG_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP0656105.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(16701..17609)
                     /gene="ycaN"
                     /locus_tag="ACHLAG_002915"
     CDS             complement(16701..17609)
                     /gene="ycaN"
                     /locus_tag="ACHLAG_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0656106.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGNPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERSGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            17709..18299
                     /gene="ycaK"
                     /locus_tag="ACHLAG_002916"
     CDS             17709..18299
                     /gene="ycaK"
                     /locus_tag="ACHLAG_002916"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFP0656107.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPFWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            complement(18381..19121)
                     /gene="pflA"
                     /locus_tag="ACHLAG_002917"
     CDS             complement(18381..19121)
                     /gene="pflA"
                     /locus_tag="ACHLAG_002917"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFP0656108.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWE
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(19313..21595)
                     /gene="pflB"
                     /locus_tag="ACHLAG_002918"
     CDS             complement(19313..21595)
                     /gene="pflB"
                     /locus_tag="ACHLAG_002918"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP0656109.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(21650..22507)
                     /gene="focA"
                     /locus_tag="ACHLAG_002919"
     CDS             complement(21650..22507)
                     /gene="focA"
                     /locus_tag="ACHLAG_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFP0656110.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         5521 a         5352 c         6011 g         5706 t
ORIGIN      
        1 ggacaattta caaaagaaag ataacccata ttgtataatg cgtttaaata tgccgtctta
       61 tataaaatga tggttagatg aatatgcgga ttatctttct acgcaaggag tatttatctc
      121 tactcccgtc aatgattgca tctcttttct ctgctaacgg tttcgcggcg gccattgatt
      181 tatgccaggg atatgatatc aaagcgagtt gtcacgccag caggcaaagc ctttcaggca
      241 ttacgcagga ctggagtgtc gccgatgggc aatggctggt tttttcggat atgaccaata
      301 acgccagcgg aggagccgta tttttgcaac aaggagcgga attttcacta ttaccagaaa
      361 atgaaactgg aatgactctg tttgccaata acaccgttac aggagaatat aataacggcg
      421 gggccatatt tgctaaagaa aactcaacgc tgaatcttac tgatgttatt ttttctggta
      481 acgtcgcagg cggctatggt ggcgcaatct attcttctgg tactaatgat actggtgccg
      541 tcgatttacg tgtcactaac gccatgtttc gcaataatat cgctaatgat ggcaaaggtg
      601 gcgcaattta taccattaat aatgacgtct atttaagtga tgttattttt gataacaacc
      661 aggcatatac atcaacaagt tacagtgatg gcgatggcgg ggcaatcgat gttaccgata
      721 ataatagcga cagcaagcat ccttcaggtt atacgataat aaataacact gcctttacaa
      781 ataacactgc cgaaggttat ggcggggcga tatataccaa tagcgcgacg gctccctatc
      841 ttattgatat ttctgttgat gacagctaca gccagaacgg cggcgtgtta gtggatgaga
      901 acaacagcgc agcaggctat ggagatggtc cttccactgc ggcgggtggc tttatgtatc
      961 tcggcttaag tgaagttacc tttgatattg ccgacggaaa aacgctggtt attggcaata
     1021 cagagaatga cggagctgtt gactctattg ctggtaccgg gttgatcacc aaaacaggtt
     1081 ccggcgatct ggtacttaat gcagataaca atgactttac tggtgagatg cagattgaaa
     1141 acggtgaagt taccctgggc cgcagcaact ccctgatgaa tgtcggcgat acgcattgcc
     1201 aggacgatcc gcaagactgc tacggtctga cgatagggag tattgataag taccagaatc
     1261 aggcagagct aaacgttggc tccacccaac aaacctttgt gcactcattg acgggctttc
     1321 agaatggcac tttaaatatc gatgctggtg gcaatgttac tgttaatcaa ggcagttttg
     1381 ctggcaccat cgaaggtgct ggtcagctca ccattgcgca aaacggcagc tacgtgctgt
     1441 cgggggcgca gtcgatggcg ctaaccggcg atatagtcgt tgatgatggt gcggtgcttt
     1501 cgctggaagg cgacgcggca gatctcgccg ctctccagga cgatccgcag tcgatcgtgt
     1561 taaacggcgg tgtgctcgat ctctctgatt tctccacctg gcagagcggc acatcataca
     1621 atgatggcct tgaagtcagt ggcagcagcg gaacggttat cggcagtcag gatgtggtag
     1681 atcttgcagg tggcgacaat ttgcatatcg gcggcgacgg gaaagatggc gtctacgtgg
     1741 tggtcgatgc gagcgacggg caggtaagtc tggcaaacaa taatagttat ttgggcacaa
     1801 cacaaatcgc ctccggtacg ctgatggtga gcgacaactc gcagcttgga gatacccact
     1861 ataaccgcca ggttatcttt accgataagc aacaagaaag cgtgatggag attactgcca
     1921 atgtcgatac tcgctctacg acgactgagc atgggcgtga tattgaaatg cgcgccgacg
     1981 gtgaagtggc agttgatgcg ggggtagaca cgcagtgggg cgcactgatg gctgacagca
     2041 gcgggcagca tcaggatgag ggtagcacat ttactaaaac gggggcgggt acgctggagc
     2101 tgaccgccag cggtacaacg cagtcggcag tacgtgtcga agaaggcacc ctgaaaggcg
     2161 atgttgcgga tatctttcct tatgcttcgt cactgtgggt cggtgatggg gcgacgttcg
     2221 ttactggcgc ggatcaggat attcagtcta tcgatgctac ttccagcggc actatcgata
     2281 tcagcgatgg tacggttttg cgtctgaccg ggcaggatac ttccgtcgcc cttaatgcct
     2341 cattgtttaa cggcgatggg acgctggtga atgccaccga tggcgtgacg ttgacaggtg
     2401 agcttaatac caaccttgaa actgacagcc tgacctatct ttccgacgtg acggttaatg
     2461 gcaatctgac caatacgtcc ggtgcggtta gcctgcaaaa tggcgtcgct ggcgatacgc
     2521 tgacggtaaa cggtgattat accggcggcg gtacgctact gctcgatagc gaattaaacg
     2581 gcgatgactc ggtaagcgat caattggtgt tgaacggtaa tactgctggc aacacaactg
     2641 tggtggttaa ctccattaca gggattggtg agccgacatc gacaggcatt aaagtggttg
     2701 atttcgcagc cgatcccacg cagtttcaaa acaatgcgaa gttcagtctg gcaggcagcg
     2761 gctacgtcaa tatgggagca tatgactaca cgctggtgga agataacaac gactggtatc
     2821 tgcgatcgca agaagtaacg ccaccattgc cacctgatcc agaccctgac ccaacacccg
     2881 atcctgaccc aacacccgat ccaatccccg cttaccagcc ggtgctgaat gccaaagttg
     2941 gcggttatct caataatctg cgggcggcaa atcaggcgtt tatgatggag cgacgcgatc
     3001 atgcaggtgg caatggtcag acgctgaatt tacgtgttat cggcggacgt tatcattaca
     3061 cggcggcagg gcaactggct cagcatgaag acacttctac ggtgcagctt agcggtgacc
     3121 tgtttagcgg gcgctggggc gatgatggcg agtggatgct tggggcggtt ggtggctaca
     3181 gtgataatca gggcgagagc cgctcgaata tgaccggaac tcgcgccgat aaccagaacc
     3241 acggttatgc cgttgggctg acctcaagct ggtatcagca cggtaatcag aagcaggggg
     3301 cctggctgga tagctggctg caatacgcgt ggtttaacaa tgatgtttcc gaacaagacg
     3361 atggcacaga tcattaccac tcgtcgggga ttatcgcctc gctggaagcc gggtatcagt
     3421 ggttaccggg gcgtggtgtg gtgattgaac cgcaggcgca ggtgatttat cagggcgtgc
     3481 agcaggatga ttttaccgcc gctaaccgtg cgcgcgtgtc acaatcgcag ggtgatgata
     3541 ttcagatgcg gctgggttta cacagcgaat ggcgtaccgc tgttcatgtc ataccaacat
     3601 tagatctgaa ttattatcac gatccccatt cgacggaaat tgaagaggat ggcagcacta
     3661 tcagtgacga tgcggtgaag caacggggtg aaataaaagt gggagtcacg ggcaatatca
     3721 gtcagcgagt gtccctgcgc ggcagcgtgg cgtggcagaa agggagtgat gattttgccc
     3781 agatggcagg gtttttgtcg atgacggtga aatggtaatc actgtctagt cggcacggtt
     3841 gccggatgcg gcgtaaacgc cttatccggc ctacggggag catttgtagg cctgataaga
     3901 cgcggcaagc gtcgcatcag gcattggctc ggccaaacaa caacctcact tattctgcgt
     3961 gtgcagcata tagttcacat ccacgccggg gccgagttta aaagtattag tgagcgggtt
     4021 gtaatgtagc ccggttatat gtcgctcttt aaggcttgtc tgatctaccc agccaagcag
     4081 ttctgccggt ttaataaact tcttcacatc atgcgtacct ttgggcacca tgcgcaaaat
     4141 atattccgcg ccaaccaccg ccatcagcca tgacttgccg ttgcggttaa gcgtcgagaa
     4201 aaagacatcg ccgcctggtt tcaccagttg cgcacaggct ctgaccactg actgcggatc
     4261 ggggacgtgc tccagcatct ccatgcaggt caccacatca tactgcccgg catgttttgc
     4321 cgcgtgctct tccacggttt cctgcacata atccacctga atgccgcttt ccagcgcgtg
     4381 cagttttgcc acctgcaatg gctcaaagcc catatccaga ccggtcaccg tcgcgccttc
     4441 gcgcgccata ctctcggcca gaatgccgcc gccacaaccg acatcgagca cctttttgcc
     4501 aaataaaccg ccagcacgct cggcaatata gcccagacgc agcgggttaa tgcggtgcaa
     4561 cggtttgaac tcaccttcaa gatcccacca gcgggaggcg acggcttcaa atttagcgat
     4621 ctcttcgtgg tctacgttat ggttttccgg cgatttttcg gcattcattg gcacttctac
     4681 tccgtaattg gcaagacaaa cgagtatatc aggcattgga tgtgaataaa gcgtataggt
     4741 ttacctcaaa ctgcgcggct gtgttataat ttgcgacctt tgaatccggg atacagtaga
     4801 gggatagcgg ttagatgagc gaccttgcga gagaaattac accggtcaac attgaggaag
     4861 agctgaagag ctcctatctg gattatgcga tgtcggtcat tgttggccgt gcgctgccag
     4921 atgtccgaga tggcctgaag ccggtacacc gtcgcgtact ttacgccatg aacgtactag
     4981 gcaatgactg gaacaaagcc tataaaaaat ctgcccgtgt cgttggtgac gtaatcggta
     5041 aataccatcc ccatggtgac tcggcggttt atgacacgat cgtccgtatg gcgcagccat
     5101 tctcgctgcg ttacatgctg gtagacggtc agggtaactt cggttccatc gacggcgact
     5161 ctgcggcggc aatgcgttat acggaaatcc gtctggcgaa aattgcccat gaactgatgg
     5221 ccgatctcga aaaagagacg gtcgatttcg ttgataacta tgacggcacg gaaaaaattc
     5281 ccgacgtcat gccaaccaaa attcctaacc tgctggtgaa cggttcttcc ggtatcgccg
     5341 taggtatggc aaccaacatc ccgccgcaca acctgacgga agtcatcaac ggttgtctgg
     5401 cgtatatcga tgatgaagac atcagcattg aagggctgat ggaacacatc ccggggccgg
     5461 acttcccgac ggcggcaatc attaacggtc gtcgcggtat tgaagaagct taccgtaccg
     5521 gtcgcggcaa ggtgtatatc cgcgctcgcg cagaagtgga agttgacgcc aaaaccggtc
     5581 gtgaaaccat tatcgtccac gaaattccgt atcaggtaaa caaagcgcgc ctgatcgaga
     5641 agattgcgga actggtaaaa gaaaaacgcg tggaaggcat cagcgcgctg cgtgacgagt
     5701 ctgacaaaga cggtatgcgc atcgtgattg aagtgaaacg cgatgcggtc ggtgaagttg
     5761 tgctcaacaa cctctactcc cagacccagt tgcaggtttc tttcggtatc aacatggtgg
     5821 cattgcacca tggtcagccg aagatcatga acctgaaaga catcatcgcg gcgtttgttc
     5881 gtcaccgccg tgaagtggtg acccgtcgta ctattttcga actgcgtaaa gctcgcgatc
     5941 gtgctcatat ccttgaagca ttagccgtgg cgctggcgaa tatcgacccg atcatcgaac
     6001 tgatccgtca tgcgccgacg cctgcagaag cgaaaactgc gctggttgct aatccgtggc
     6061 agctgggcaa cgttgccgcg atgctcgaac gtgctggcga cgatgctgcg cgtccggaat
     6121 ggctggagcc agagttcggc gtgcgtgatg gtctgtacta cctgaccgaa cagcaagctc
     6181 aggcgattct ggatctgcgt ttgcagaaac tgaccggcct tgagcacgaa aaactgctcg
     6241 acgaatacaa agagctgctg gatcagatcg cggaactgtt gcgtattctt ggtagcgccg
     6301 atcgtttgat ggaagtgatc cgtgaagagc tggagctggt tcgtgaacag ttcggtgaca
     6361 aacgtcgtac tgaaatcacc gccaacagcg cagacatcaa cctggaagat ctgatcactc
     6421 aggaagatgt ggtagtgacg ctctctcacc agggctacgt taagtatcag ccgctttctg
     6481 aatacgaagc gcagcgtcgt ggcgggaaag gtaaatctgc cgcacgtatt aaagaagaag
     6541 actttatcga ccgactgctg gtggcgaaca ctcacgacca tattctgtgc ttctccagcc
     6601 gtggtcgcgt ctattcgatg aaagtttatc agttgccgga agccactcgt ggcgcgcgcg
     6661 gtcgtccgat cgtcaacctg ctgccgctgg agcaggacga acgtatcact gcgatcctgc
     6721 cagtgaccga gtttgaagaa ggcgtgaaag tcttcatggc gaccgctaac ggtaccgtga
     6781 agaaaactgt cctcaccgag ttcaaccgtc tgcgtaccgc cggtaaagtg gcgatcaaac
     6841 tggttgacgg cgatgagctg atcggcgttg acctgaccag cggcgaagac gaagtaatgc
     6901 tgttctccgc tgaaggtaaa gtggtgcgct ttaaagagtc ttctgtccgt gcgatgggct
     6961 gcaacaccac cggtgttcgc ggtattcgct taggtgaagg cgataaagtc gtctctctga
     7021 tcgtgcctcg tggcgatggc gcaatcctca ccgcaacgca aaacggttac ggtaaacgta
     7081 ccgcagtggc ggaataccca accaagtcgc gtgcgacgaa aggggttatc tccattaagg
     7141 ttactgaacg taatggttta gtggttggcg cggtacaggt agatgactgc gaccagatca
     7201 tgatgatcac cgatgccggt acgctggtac gtactcgcgt ttcggaaatc agcatcgtag
     7261 gccgtaacac ccagggcgtg atcctcatcc gtactgcgga agatgaaaac gtagtgggtc
     7321 tgcaacgtgt tgctgaaccg gttgacgagg aagatctgga taccatcgat ggcagtgccg
     7381 cggaagggga cgatgaaatc gctccggaag tggacgttga cgacgagcca gaagaagaat
     7441 aattttactt cttcatgcca aaagggagct atctcccttg tttgaattga aaagtccagg
     7501 ctgcaaagtc tgggcttttg tcgtattagg gcacggtaaa gtttggctgt gcccgtaaaa
     7561 aatggctggc tatacacaag gaatgtggca atgagtggtg aaaaaaaggc gaaaggctgg
     7621 cggttctatg gtcttgtagg ttttggcgca atagcactgc tttccgctgg cgtctgggcg
     7681 ttgcaatatg ctggcagtgg gccagaaaaa acgttgtcgc cgctggtggt gcacaacaat
     7741 ctgcaaatcg atctcaatga gccggacctc tttctcgaca gcgactctct gagccagctt
     7801 cccaaagatc tcctcaccat tccgtttctc cacgatgttc tgagcgaaga tttcgttttc
     7861 tattatcaga atcatgccga tcgtctgggc attgaaggca gcattcgtcg tattgtctat
     7921 gaacacgatc tcacgctgaa agataagctc ttttcgtcac tcttagatca gcccgcgcag
     7981 gcagcgctgt ggcacgataa acaaggccat ctttcacatt atatggtgct gatccagcgc
     8041 agtggtttaa gcaaactgct ggagccattg ttgtttgccg ctaccagcga cagccagtta
     8101 agcaaaacgg aaatcagtag catcaagata aatagtgaaa ctattcctgt ttatcagttg
     8161 cgctataacg gcaataacgc cctgatgttc gcgacttatc aggacaagat gctggtgttt
     8221 tccagcacgg atatgttgtt taaagatgat cagcaggata ccgaagccac ggcgatcgca
     8281 ggtgatttgt tgagcggcaa aaaacgctgg caagccagct tcggtctgga agagcgtact
     8341 gccggaaaaa caccagtacg ccagcgcatc gtggtcagcg ccaggttgct gggttttggc
     8401 taccagcgat taatgccttc ttttgccgga gtgcgtttcg aaatgagcaa cgacggctgg
     8461 catagctttc tggcgttaaa tgatgaatcc gcgagtgtgg atgccagttt cgattttacg
     8521 ccggtctgga acagtatgcc tgccggggcc agcttctgtg tggcggtgcc gtattcacac
     8581 ggtattgccg aagagatgct ttcgcacatc agccaggaaa acgacaagct gactggggcg
     8641 ttagacggtg ccgcggggct gtgctggtat gaagactcaa aattgcaaac cccgctgttt
     8701 gtcggtcagt ttgatggcac tgccgaacaa gcgcaattgc cagggaaact gtttacgcaa
     8761 aatattggtg cgcacgaaag caaagcgcct gcgggcgctt tttttgtctc cctttgatac
     8821 cgaacaataa ttactcctca cttacacgta atactacttt cgagtgaaaa tctacctatc
     8881 tctttgattt tcaaattatt cgatgtatac aagcctatat agcgagctgc tatagaaata
     8941 attacacaat acggtttgtt actggaatca atcgtgagca agcttgagtg agccattatg
     9001 aaaacgaaaa tccctgatgc ggtattggct gctgaggtga gtcgccgtgg tttggtaaaa
     9061 acgacagcga tcggcggcct ggcaatggcc agcagcgcat taacattacc ttttagtcgg
     9121 attgcgcacg ctgtcgatag cgccattcca acaaaatcag acgaaaaggt tatctggagc
     9181 gcctgtacag ttaactgtgg tagtcgctgc ccgctacgta tgcacgtcgt ggacggtgaa
     9241 atcaaatatg tcgaaacgga caataccggc gatgacaatt acgacggcct gcaccaggtt
     9301 cgcgcctgcc tgcgtgggcg ttccatgcgt cgccgtgtct acaatccgga ccgcctgaaa
     9361 tatccgatga aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat tagctgggaa
     9421 gaagcctacg acatcatcgc gaccaatatg cagcgcctga tcaaagagta cggcaacgag
     9481 tctatctatc tgaactatgg caccggtacg ctgggcggca ccatgacccg ctcctggccg
     9541 ccgggaaata ccctggtcgc gcggctgatg aactgctgcg gcggctatct gaaccattac
     9601 ggcgactact cctccgcgca aattgcggaa ggtttgaact atacctacgg cggctgggca
     9661 gatggtaaca gcccgtcgga tatcgaaaac agtaagctgg tagtgctgtt tggtaataac
     9721 cctggcgaaa cgcgaatgag tggcggtggg gtgacttact atcttgaaca ggcacgccag
     9781 aaatctaatg cccgcatgat catcattgat ccgcgctata ccgacaccgg tgccgggcgc
     9841 gaagatgagt ggatccctat tcgtccggga acagatgccg cactggttaa cggtctggcg
     9901 tacgtcatga tcactgaaaa cctggtggat caggcattcc tcgataaata ttgcgttggc
     9961 tacgatgaga aaaccctgcc agccagtgcg ccgaaaaatg gccactataa agcttatatt
    10021 ctgggtgaag ggccagatgg cgtggctaaa acaccggaat gggcctcaca aatcaccggt
    10081 gttccggcag acaaaatcat caaactggct cgtgaaatcg gcagtaccaa accggcgttt
    10141 atcagccagg gatggggccc gcagcgtcac gctaacggtg aaatcgcaac ccgtgctatc
    10201 tcgatgctgg cgattctgac cggtaacgtt ggtattaacg gaggcaacag cggcgcgcgt
    10261 gaaggttcat acagcttacc gtttgtccgt atgccgacct tggaaaaccc gatccagacc
    10321 agcatttcga tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat gacggcgctg
    10381 cgtgatggtg tacgcgggaa agataagctg gatgtgccga tcaaaatgat ctggaactat
    10441 gccggtaact gcctgattaa ccagcattct gaaatcaacc gtacccatga aatccttcag
    10501 gatgataaga agtgcgagct gattgtggtt atcgactgcc acatgacctc atcggcgaaa
    10561 tatgctgaca tcctgctgcc tgactgcagc gcttccgaac agatggactt tgcactggat
    10621 gcatcctgcg ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa accgcgcttt
    10681 gaatgtaaga ccatctatga aatgaccagc gaactggcaa aacgtcttgg cgttgagcaa
    10741 cagtttactg aaggccgtac ccaggaagag tggatgcggc atctgtatgc ccagtcgcgg
    10801 gaagcgattc ctgaactgcc aacgtttgaa gagttccgca agcaggggat ctttaaaaag
    10861 cgcgacccac aagggcatca cgttgcttat aaagccttcc gtgaagatcc gcaggcaaac
    10921 ccactgacta cgccatcggg caaaattgag atttattcgc aggcgctggc tgacattgcc
    10981 gctacctggg aattgccaga aggcgatgtg atcgatccac tgccgatcta cacgccgggc
    11041 tttgaaagtt atcaggatcc gctgaacaaa cagtatccgc tgcagcttac gggtttccac
    11101 tataaatctc gcgttcactc aacttacggc aacgttgatg tgctgaaagc ggcttgccgt
    11161 caggaaatgt ggatcaaccc gcttgatgcg caaaaacgcg gtatcaacaa cggcgataaa
    11221 gtcaggatct ttaacgatcg tggtgaagtt catattgagg cgaaagtgac gccacgaatg
    11281 atgccgggtg tggtcgcact gggtgaaggt gcctggtatg acccggatgc aaaacgtgtc
    11341 gataagggcg gttgtattaa cgtactgacc actcaacgtc cgtctcctct cgctaagggg
    11401 aatccgtcac atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac cgatgacaac
    11461 ccagtatgga ttttttattg attccagccg ttgcaccggt tgcaaaacct gcgagctggc
    11521 ctgtaaagac tacaaagatt tgacgccaga agtcagcttc cgccgcattt atgaatatgc
    11581 tggcggcgac tggcaggaag ataacggtgt ctggcaccag aacgtgtttg cctactatct
    11641 gtcgatttca tgtaaccact gcgaagatcc ggcttgtact aaagtctgct cgagcggtgc
    11701 gatgcataaa cgcgaagatg gttttgttgt ggtcgatgaa gatgtatgca ttggctgccg
    11761 ctactgtcat atggcttgcc cgtatggcgc accgcaatat aacgaaacga aaggccatat
    11821 gaccaaatgc gatggttgtt atgaccgtgt tgcggagggt aaaaagccga tctgtgttga
    11881 atcttgcccg ctgcgggcgc tggatttcgg tcctatcgac gagctgcgta aaaaacatgg
    11941 cgatctggcg gccgttgcgc cgttgccgcg agctcacttt accaaaccga atattgtgat
    12001 caaacccaat gccaacagcc gcccgaccgg ggataccact ggctacctgg caaacccgaa
    12061 ggaggtgtga gatgggaagt ggatggcatg aatggccgct gatgatcttc acggtcttcg
    12121 ggcaatgtgt agcgggtggt tttatcgttc tggctttggc gctgctcaaa ggcgacctgc
    12181 gagcagaagc ccagcagcgt gttatcgcct gcatgtttgg cttatgggtg ctgatgggca
    12241 ttggctttat cgcctctatg ctccatcttg gttcaccgat gcgcgccttt aactcgctca
    12301 accgggtagg ggcttcagca ctcagtaacg aaatcgccag cggttcgatc ttttttgctg
    12361 taggcggcat cggctggttg ctggcaatgc tgaaaaagct gtcaccggca ttgcgtacgc
    12421 tatggctggt ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg gtgcgtgtgt
    12481 ataacagcat tgataccgtt ccgacctggt acagcatctg gacgccgatg ggcttcttcc
    12541 tgacgatgtt catgggcggc ccgttactgg gttacctgtt gctgagtctg gctggcgtcg
    12601 atggctgggc gatgcgtctg ctgccagcca tttctgtact ggcactggtg gtgagtggtg
    12661 tgatgtcagt gatgcagggc acagagctgg caactattca tagttctgtg cagcaggccg
    12721 cagcgctggt gccggactat ggtgcgctga tgtcctggcg gatcgtgctt ttggccgttg
    12781 ccctgtgctt gtggattgca ccacagctaa aaggttatca gcctgcggtt ccgctactat
    12841 ccgtctcatt cattctgctt ctggcagggg aattaatcgg tcgcggcgta ttctatggtt
    12901 tgcatatgac cgtggggatg gccgtcgcaa gctaatcagt aacaaccggg gtttcggccc
    12961 cggttctctt ttatttctgc ttcgttaacg tgtcataact ggtcatcaaa ttacgataat
    13021 caggaatatg gttagagaac aacgtcgcca gtccttcaat atcattacgc cagtcacggt
    13081 gcagctcgca agccacgccg aaccatgtca tcaattgcgc acctgcttgc gacatgcgat
    13141 cccatgccga atgccgggta atttcattaa aggtccctga cgcgtcagtc acaacaaaga
    13201 catcaaaccc ttcttcaatg gctgaaagcg ccgggaatgc cacgcaaact tcggttacca
    13261 caccggcaat aattaactgt tttttacctg tcgctttgac cgcttttaca aaatcttcgt
    13321 tatcccaggc gttaatattt cccgggcgag caatgtaagg tgcatcgggg aattgtgctt
    13381 tcagttctgg aactaatggg ccgttaggac cggtttcaaa actggtggta agaatagttg
    13441 gtaagttgaa atacttagcc aggtcaccca gcgccagcac gttattttta aacttatcgg
    13501 gttcgatatc ccgtacaagg gaaagtaaac cagcctggtg atcaacaagc aaaacggcag
    13561 catcattttt atcaagacga acatacggtt tggtcatcct cttctccttt cgagagtcag
    13621 tggtacgtct gaaatcggtc agacgcgatg cattgctctg aaagcataga cgggaaatat
    13681 gagtttgctg tgaccatgaa atttttcgac tgaacgaagc gtatgaaatt tgtgttagat
    13741 caataaaaac aatcagatga gattaatcgt ctcttcagca ttagcgttat gaatgagtaa
    13801 tataaatcga taataccgcc gctgcgatat gcgcgtattt tcacccattg acaatgtttt
    13861 tggcggtggc atgatgcgca tgaaatttga acttcctcac ggttttaatt catgtccacg
    13921 tatacccggc ctgtcatgct tttgctgtct ggcctgcttt tgttgactct ggcgatcgcg
    13981 gtgttaaata cactcgtgcc gctttggctc gcccaggaac acatgtccac atggcaggta
    14041 ggcgttgtca gctcatccta ttttaccggc aaccttgtcg gtacattgct gacagggtat
    14101 gtcattaagc gcattggctt taaccgcagc tattatctgg cctccttcat ttttgccgct
    14161 ggctgtgccg gccttggcct gatgattgga ttctggagct ggttggcttg gcgttttgtc
    14221 gcgggcgtcg gctgtgccat gatttgggtg gttgttgaga gcgcgctgat gtgcagtggg
    14281 acgtcacgta accgtgggcg tttgcttgct gcgtatatga tggtttatta cgtgggaacg
    14341 tttttaggcc agttactggt cagcaaagtt tcaaccgagc tgatgtccgt attgccgtgg
    14401 gttacaggtt tgacgttggc agggatctta ccgctgttgt ttacgcgtgt gctgaatcag
    14461 caggctgaaa accatgattc gacgtcaatt acgtcaatgc taaaactccg tcaggcgcgg
    14521 cttggcgtga atggctgcat tatctcagga atcgttctgg gatctctata tggcctgatg
    14581 ccgctgtacc tcaatcacaa aggggtgagc aatgccagca ttggtttctg gatggcggta
    14641 ctggtcagtg cgggtatcct tggacaatgg ccgattggac gtctggcgga taagtttggt
    14701 cgactgctgg tgttgcgtgt tcaggtcttt gtcgtcattc tcggcagtat cgcgatgctt
    14761 agccaggcgg cgatggcccc agcgttattc atcctcggtg ccgctggctt tacgctatat
    14821 ccggtggcga tggcatgggc ttgcgagaaa gttgaacatc atcaactggt ggcgatgaac
    14881 caggccttac tgttgagtta tactgtggga agtctgcttg gcccgtcatt taccgctatg
    14941 ctaatgcaga atttctccga taatttattg tttatcatga tcgccagcgt atcgtttatc
    15001 tatttgctga tgctgctgcg caacgccggt catacgccga aacccgttgc tcacgtgtaa
    15061 atgaattcaa gcagagtgtg aacttactgt ttcacactct gcttttttgt ttcttctatc
    15121 tgacttgctt tattccaaat tttattcgtt taaaaataaa atgtgcagca ggttataatt
    15181 ttgcatttcg ctatttccgc acttcttatt tgccgcgcat aatccctcgt tttacagatg
    15241 cccctttaat tttggcgaag gatttgtcta tggctgggaa tgttcaggaa aaacagttgc
    15301 gatggtacaa cattgcgctg atgtctttta tcactgtctg gggttttggc aacgttgtta
    15361 ataactatgc caaccagggg ctggtggttg ttttttcatg ggtgtttatc tttgcactct
    15421 atttcacacc ttatgcgcta attgttggtc agttaggctc gaccttcaaa gatgggaagg
    15481 gcggggtcag tacctggatt aaacacacga tgggacccgg actggcttat ctcgccgcgt
    15541 ggacctactg ggtggtgcat attccctatc tggcacaaaa accccaggca attctgattg
    15601 cgctcggttg ggcgatgaaa ggcgacggtt cgctaatcaa agaatattca gtcgtagcgt
    15661 tacaggggtt aacgctggtg ctgtttatct tctttatgtg ggttgcttca cgcggtatga
    15721 aatcgctgaa aatcgtcggt tctgtggcag ggattgcgat gtttgttatg tcgctcctgt
    15781 atgtggcgat ggcggtaacc gcgcctgcga ttactgaagt gcatattgcg accacaaaca
    15841 ttacctggga aacgttcatt cctcatatcg actttaccta cattaccact atttcaatgc
    15901 tggttttcgc ggttggcgga gcagagaaga tttctcctta cgttaatcaa acgcgcaacc
    15961 caggaaaaga atttccaaaa gggatgctat gcctggcggt gatggttgcg gtttgtgcca
    16021 ttctgggctc actggcgatg gggatgatgt ttgactcgcg taatatcccg gatgacttaa
    16081 tgactaacgg tcaatattac gcctttcaga agctgggcga gtattacaac atgggtaata
    16141 ctttaatggt gatttacgcc attgcgaata ccctgggaca agtagctgcg ctggtattct
    16201 cgattgatgc cccgcttaaa gtgctattag gtgatgctga cagcaaatat attccagcca
    16261 gtttatgtcg taccaacgct tctggtacgc ccgttaatgg ctattttctg accctggtgc
    16321 tggtggcaat tctgattatg ctgccgactc tcggcatcgg tgatatgaac aatctctata
    16381 aatggctgtt gaatcttaac tcggtagtga tgccgctgcg ttatttatgg gtatttgttg
    16441 catttattgc agtcgttcgc ctggcgcaga aatataaacc agaatatgtc tttattcgta
    16501 ataagcctct ggcaatgacc gtcgggattt ggtgttttgc ctttaccgcc tttgcctgct
    16561 tgacggggat cttcccgaaa atggaagcct tcactgcaga gtggaccttc cagttggcgc
    16621 tgaatgttgc aacgccgttt gtgctggtag gattaggact gatattcccg ctgctggcgc
    16681 gtaaagcgaa tagtaaataa ttattgtggt cggtgacgct gactctggca gatctgaccc
    16741 gtttttacat aatcgagaaa tgcgcgcaag ccgcaggaca tatactggcg gtttggataa
    16801 tagatctgga agcctgaacg ctctgtgctc cagtcttcca gtacacattc taaacgacca
    16861 gtatcaaggt actccttgat ctgttcatag agcagatacc cgataccagc acccatcaaa
    16921 acggcctcca gttcagcatc aacatcatcc agaatgatat ttcctgcgac agcaatttcc
    16981 agctcctttg caaattgcca gtggaaaggt ttgccgctgg gataacgaaa aacaacacat
    17041 tgatgattaa gcagatcatg aggatggcgg gggttgccat atcttgcaaa gtactctggc
    17101 gttgctgcta cacataattt cactggtggg ccgatcgcca cgctgatcat atctttttcg
    17161 acgatgccac taagtcgaac gccagcgtca aagccctgtt ggacgatatc gacaagagaa
    17221 tcgtcagttg tgagctcgac tttaatatca ggatattcac gtgtgaaacc aaccagtaaa
    17281 ggcattaaaa atatacgtgc agctacccgg gcggcattga ttttgagcgt tcctgtcggt
    17341 gtcagacgaa aatcattcat ctcatctaac atgatctgaa tttcgtcgaa agctgggcgc
    17401 aatcgttcat agagattaga acccgcttcc gttaacgata cgcttcgggt tgtccgattg
    17461 aataagcgaa ttttaagacg ttgttccagt gttttaatac tatggctaat ggcggacgag
    17521 gataagccta attcatcgcc cgctgcacga aagctttgat tacgggccac ggcaaagaaa
    17581 gtggcaaagt cagacatatt catccgcatt agtgaaaatc cttcattaac tcatcctgaa
    17641 tcatcacgtt tacaggatta tgccgcctgc gtaaagtgcg ctccagaact taacgtggag
    17701 gtaaaattat gcagtctgaa cgtatttatt tggtatgggc ccatcctcgt catgattcat
    17761 tgaccgcaca tattgctgat gcgatccatc agcgggcaat ggagcggaaa atacaggtga
    17821 cggaactcga tttatatcgg cgtaatttca acccggtgat gacgtcggaa gatgaaccag
    17881 actggaagaa tatggataaa cgttattctc cagaggttca tcagctttat tcagagctgc
    17941 ttgaacatga cacgttagtg gtggtttttc ctttctggtg gtacagcttc ccggcaatgc
    18001 taaaaggata tattgacaga gtatggaata atgggctggc ttatggagat gggcacaaat
    18061 taccattcaa taaagttcgt tgggtggcgc tggttggagg agacaaagaa tcatttgtcc
    18121 agatgggctg ggaaaaaaat ataagcgatt atttgaaaaa tatgtgcagt tatcttggta
    18181 ttgaagatgc cgatgtcact ttcttgtgta atacagtggt attcgatggg caagaacttc
    18241 atgcgagcta ttatcagtcg ttattatctc aggtacggga tatggtagat gcactataaa
    18301 atgtgttaaa aacgctgtag cagaatgaag cgcggaataa aaaagcggca actcaataaa
    18361 gttgccgctt tacggggaaa ttagaacatt accttatgac cgtactgctc aagaatgcct
    18421 ttcacgcgtt ccatggtctc tttcttaggt ggtttaacac cgtcgagttt gtactcttca
    18481 cccattgcca cccatttgtg tttgcccagc tcgtggtagg ggaggagttc gattttctca
    18541 acgttgccca tatcacgggt aaattcaccg aggcgatgcg ctgaatcgtc atcgtcagac
    18601 cagcctggga caacaacgta gcggatccac accttcacat ttttgttcgc cagatatttg
    18661 gcgaactcca gcgtgcggtg gttggaaact ccaaccagat tttggtggat ctcgtcgttc
    18721 atctgtttga gatcgagcat taccaggtcg gttacttcca gcagttcatc aatcaccgga
    18781 tcgtaacgac gaacaaaacc gttggtgtcc agacaggtat gaatgccttc ttttttgcag
    18841 gcgcggaacc agtcacgaac aaactcagct tgtaggattg cctcaccgcc ggatgcggta
    18901 acgccgccgc cggaagcgtt cataaagtgg cgataggtca ccacctcttt cattaaatct
    18961 tcaacggtaa cttctttacc gccatgcgtt tcccaggtgt cgcggttatg acaatacagg
    19021 cagcgcatca ggcagccctg gaaaaaggtg ataaagcgaa tacctgggcc gtctacggtt
    19081 ccacaggatt caaaggagtg aatgcgacca ataactgaca ttgcggtgtt tctccagatg
    19141 tggcccatct gaggccgtgt tggtgcgcag ctcgaaggct acgtcgagtc tgttttggca
    19201 gtcaccttaa agtatagata gctgacaaaa aaggctctcg tgctaaaaaa ggccccactt
    19261 tcgtggagcc tttattgtac gctttttact gtacgatttc agtcaaatct aattacatag
    19321 attgagtgaa ggtacgagta ataacgtcct gctgctgttc tttagtcagc gagttgaaac
    19381 gtactgcgta gccagataca cggatggtca gctgcggata tttttccggg ttttccatcg
    19441 cgtcgagcag catttcacgg ttcatcacgt taacgttcag gtgctgacca ccttcgatgg
    19501 atgcttcgtg gtggaagtaa ccatccatca gaccagccag gttggtctta cgaacttcgt
    19561 cgtctttacc cagtgcgttc ggaacgatag agaaggtgta ggagatacca tctttagcgt
    19621 aagcaaacgg cagtttagca acggaagtca gagacgctac agcacctttc tggtcacgac
    19681 cgtgcatcgg gttagcaccc ggtccgaacg gcgcgccagc acgacgaccg tctggggtgt
    19741 taccagtttt cttaccatac acaacgttag aagtgatggt cagaacagac tgagtcggga
    19801 tagcgtcacg gtaggtgtgc agtttctgaa ttttcttcat gaaacgttct accaggtcaa
    19861 cagccaggtc atctacacgc ggatcattgt taccaaactg cgggtattcg ccttcgattt
    19921 cgaagtcgat agccagaccg tcttcgtcac gaatcggttt aactttcgca tatttgattg
    19981 cagacaggga gtcagcagca acggacagac cagcgatacc acacgccatg gtgcggataa
    20041 cgtcacggtc gtgcagcgcc atcagagagg cttcgtagct gtacttgtcg tgcatgtagt
    20101 ggatgatgtt cagtgcagtg atgtactgtt tagccagcca gtccatgaag tgatccatgc
    20161 gctccatcac ttcatcatag ttcaggacat cgcctttgat cggttcagac ttcggaccaa
    20221 cctgcatttt cagtttttcg tcaacgccgc cgttgattgc gtacagcatg gttttcgcca
    20281 ggtttgcacg cgcaccgaag aactgcattt gtttaccaac gatcatcggg cttacgcagc
    20341 aagcaatagc gtagtcatcg ttgttgaagt ccggacgcat caggtcatcg ttctcatact
    20401 gcagagaaga ggtgtcgatg gacactttag cggcgaattt cttgaagttc agcggcagtt
    20461 tttcagacca cagaatggtc atgttcggtt ccggagacgg acccatggtg tacagggtgt
    20521 tcaggaaacg gaagctgttt ttggtaacca gggtacgacc gtcgaggccc ataccaccga
    20581 tagattcggt tgcccagatc gggtcgccag agaacagttc atcgtattcc ggagtacgca
    20641 ggaagcgaac catacgcagt ttcatgacca ggtggtcaac catttcctgc gcttcttgtt
    20701 cggtgatctt gccagctttc aggtcacgtt cgatgtacac atccaggaag gtggaggtac
    20761 gaccgaagga cattgcagca ccgttctgag acttaacagc agccaggtag ccgaagtaag
    20821 tccactggat agcttcctga gcgttggtag ccggaccaga gatgtcgtag ccgtatttcg
    20881 cagccatttc tttcatctga cccagagcgc ggtgctgttc agcgatttct tcgcgcagac
    20941 ggatagtctg ttccaggttt acgccgtttt ccagatcagc ctgcagagaa gtgaactgtg
    21001 ccagtttgtc tttcatcagg tagtcgatac cgtacagcgc aacgcgacgg tagtcaccga
    21061 tgatacggcc acggccatat gcatctggca gaccggtcag aacaccagat ttacggcaac
    21121 gcaggatgtc cggagtgtaa acgtcgaaca cgccctggtt gtgagtttta cggtattcag
    21181 tgaagatttt tttgatcatc ggatccagtt cgcggttgta cgctttgcag gaaccttcga
    21241 tcattttgat accaccgaac gggataagag cacgtttcag cggagcttca gtctgcagac
    21301 caacgatttt ctcaagctgc ttgttgatgt agccagcgtc gtgagaggtg atggtggaag
    21361 caacagcggt gtcaaagtca actggcgcgt gagtgcggtt ttccagttta acgccttcca
    21421 ttactttgtc ccacagggtg gtggtcgctt cagtagcgcc agccaggaag gactcgtcac
    21481 cctcgtacgg agtgtagttt ttctgaatga agtcacggac gtttacttca ttctgccagt
    21541 cacctttggt aaaaccttcc caggctgtgg ctaacttttc attaagctcg gacatgtaac
    21601 acctaccttc ttaagtggat tttttattta ctgcgtactt cgacaaccat taatggtgat
    21661 cgttttcacg caggtaaatg acccagtatg tcaacccaac caacaaacca ccgccgataa
    21721 tattaccgat cgtaaccgga atcaggttat cagtgatgaa gttcatcacg gtcaggtgag
    21781 aaaaattttc cggtgcagaa ccgacagcgg tccagaattc cggagatgcg aagtcgcgga
    21841 ttacaatacc catcgggatc ataaacatgt ttgcgatact gtgctcaaaa ccgctggcaa
    21901 caaacatcgc gaccggcagc accataatga acgctttgtc catcaggcta cggccagaat
    21961 aactcatcca tactgccaga cataccatca ggtttgccag gataccaagg cagacggcct
    22021 caataaaagt atggtgcact ttgtggtcgg cggtttgtag gacgtttagt ccccattgac
    22081 catttgcggt catatactcg ccggaaagcc acattaaaag tacaaacagc agtgcgccga
    22141 ccaggttgcc aaaatagaca tttagccagt ttttcgccaa ctgaccccag gtgatgcgcc
    22201 cactcgcctt agcaacaaca atcaacactg tggaagtaaa gagatcggct ccgcagacaa
    22261 cacaaagaat cagccccaga gagaagcaaa tgccgccaac cagttttgcc atgccgaagg
    22321 gcattgtgcc tgtgccagtg gttgctgtga tatagaagac gaatgcgatt gagatgaaaa
    22381 caccggcggt aatcgccaga tagaaagtct taagcggatg tttcgttgct ttatagacac
    22441 ccgcctcttc ggccactttg gccattgcag caggaagtaa aagatcaaaa gggttgtcag
    22501 ctttcacact aactctctct ttattaagtc ggcgacgaga tactaacaaa gcattataga
    22561 tgagaaattg atatagatca tatctcgcct
//
LOCUS       DAZGVQ010000079        22219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000079 DAZGVQ010000000
VERSION     DAZGVQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.080"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            364..4356
                     /locus_tag="ACHLAG_002920"
     CDS             364..4356
                     /locus_tag="ACHLAG_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
                     /protein_id="HFP0656111.1"
                     /translation="MNKIYSLRVNDRKELVAVPETVGGRKKSSGIPGAGMLSRLLLAS
                     GAVAGVLFSSPALASVVGNTLPWQTYRDFAENKGAFHAGATNIPLYGKNGAVSGRLDK
                     APMMDFSVVDQILGVATLISPQYVAGVKHNGSYNTVRFGYADDTTYRLVDRNEHWRDF
                     HTPRLNKLVTEVAPVSVTDAGTGKGVYQNRSRYPVFYRMGSGTQYTGAASGALTRIAD
                     AYVWRTGGTVGSPVISDWSLVSNPGNLYQGVNGPLASYGTSGDSGSPLFAWDAVKKQW
                     VFVAVLHGYAGENGKTNWFTVIPAGDVNNTIKQDNSGTVVPAVAGGDIVWKYSKGSGE
                     GTLSQGGQVWKMNGFRGGSLNDGKDITFGGKGTVVLKDDVVQGAGSLTFNGDYTVRPE
                     GNQTWVGGGIIVNDGHRVDWMVNGLAGDALHKIGKGILVVAGSGENPGALNTGDGTVI
                     LAQKADAAGKVRAFSEVSIVSGRPVVVLQDSHQIEGDRIRWGYRGGTLDINGNDMAFN
                     RLAAADEGAVLTSRERPATVRLDFSQSGQKAVMWHGHFIGNLSVLNNTSSAVDFIMDG
                     GADMSGSFTQQGGGLYIQGHPVVHAVSSEAVATALRKQGDNSVLTQPVSFAQKDWESR
                     TFSIGQLKLKGAAFSLSRNATLTGDIDADNATMVLGSDSLYLDMKDGTGSSSAPVKGT
                     SAVGGASGSSTFRGNVNMRHSALTVRDHFTGSITASDSRIAVSSENVRLEGHSRLTSS
                     ALTVSDGGRLHVKGGLETDGGVTLDRGILLVDGGSVRNDVYERLLAWSEERGGLNGSG
                     EYDFMTGAAGLLRSYVRGSAGNVNLQNAAWMMTGNSSVKHLESSGSALYFSRPGGEFH
                     TLTAGSMDISDSVLVMRTDLNHSDQLRVTESLRGKNNLLLVDFTERSDGQKALNIPLV
                     TAPAGTGADVFSVKTRDTGFSHITPVVRAEQGTGGTAWQLNVVQPETAAEPVVTRQDA
                     ETPNPVEAVSPLPSPVSAPSPAPEASVTDVLTGENAGESGEYRDETRWLLTGYRSTVN
                     TSAVRDAGMLMSMGHRNFINEVNNLNKRMGDLRDINGEAGAWARIMSGTGSAGGGFSD
                     NYTHVQVGADKKHELDGLDLFTGVTMTYTDSNAGSDTFSGKTKSVGAGLYASALFDSG
                     AYIDLIGKYVHHDNEYTATFAGLGTKDYGSHSWYAGAEVGYRYHVTEDAWIEPQAELV
                     YGAVSGKQFSWKDQGMNLTMKDKDFNPLIGRTGVDVGKSFSGKDWKVTARAGLGYQFD
                     LLANGETVLRDASGEKRIKGEKDGRMLMSVGLNAEVRDNIRFGLEFEKSAFGKYNVDN
                     AVNASFCYSF"
     gene            5084..5209
                     /locus_tag="ACHLAG_002921"
                     /pseudo
     CDS             5084..5209
                     /locus_tag="ACHLAG_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(5269..5775)
                     /gene="yciE"
                     /locus_tag="ACHLAG_002922"
     CDS             complement(5269..5775)
                     /gene="yciE"
                     /locus_tag="ACHLAG_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP0656112.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(5821..6321)
                     /gene="yciF"
                     /locus_tag="ACHLAG_002923"
     CDS             complement(5821..6321)
                     /gene="yciF"
                     /locus_tag="ACHLAG_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP0656113.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(6407..6586)
                     /gene="yciG"
                     /locus_tag="ACHLAG_002924"
     CDS             complement(6407..6586)
                     /gene="yciG"
                     /locus_tag="ACHLAG_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP0656114.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(6967..7773)
                     /gene="trpA"
                     /locus_tag="ACHLAG_002925"
     CDS             complement(6967..7773)
                     /gene="trpA"
                     /locus_tag="ACHLAG_002925"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFP0656115.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(7773..8966)
                     /gene="trpB"
                     /locus_tag="ACHLAG_002926"
     CDS             complement(7773..8966)
                     /gene="trpB"
                     /locus_tag="ACHLAG_002926"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFP0656116.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(8978..10339)
                     /gene="trpCF"
                     /locus_tag="ACHLAG_002927"
     CDS             complement(8978..10339)
                     /gene="trpCF"
                     /locus_tag="ACHLAG_002927"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFP0656117.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGTGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(10340..11935)
                     /gene="trpD"
                     /locus_tag="ACHLAG_002928"
     CDS             complement(10340..11935)
                     /gene="trpD"
                     /locus_tag="ACHLAG_002928"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFP0656118.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLHVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(11935..13497)
                     /gene="trpE"
                     /locus_tag="ACHLAG_002929"
     CDS             complement(11935..13497)
                     /gene="trpE"
                     /locus_tag="ACHLAG_002929"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFP0656119.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(13519..13612)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(13589..13633)
                     /gene="trpL"
                     /locus_tag="ACHLAG_002930"
     CDS             complement(13589..13633)
                     /gene="trpL"
                     /locus_tag="ACHLAG_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFP0656120.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            13771..14652
                     /gene="rnm"
                     /locus_tag="ACHLAG_002931"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             13771..14652
                     /gene="rnm"
                     /locus_tag="ACHLAG_002931"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFP0656121.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            14649..15269
                     /gene="yciO"
                     /locus_tag="ACHLAG_002932"
     CDS             14649..15269
                     /gene="yciO"
                     /locus_tag="ACHLAG_002932"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFP0656122.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            15370..16242
                     /gene="rluB"
                     /locus_tag="ACHLAG_002933"
     CDS             15370..16242
                     /gene="rluB"
                     /locus_tag="ACHLAG_002933"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFP0656123.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(16282..16872)
                     /gene="cobO"
                     /locus_tag="ACHLAG_002934"
     CDS             complement(16282..16872)
                     /gene="cobO"
                     /locus_tag="ACHLAG_002934"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFP0656124.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(16869..17627)
                     /gene="yciK"
                     /locus_tag="ACHLAG_002935"
     CDS             complement(16869..17627)
                     /gene="yciK"
                     /locus_tag="ACHLAG_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFP0656125.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            17847..18896
                     /gene="sohB"
                     /locus_tag="ACHLAG_002936"
     CDS             17847..18896
                     /gene="sohB"
                     /locus_tag="ACHLAG_002936"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFP0656126.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(18932..19183)
                     /gene="yciN"
                     /locus_tag="ACHLAG_002937"
     CDS             complement(18932..19183)
                     /gene="yciN"
                     /locus_tag="ACHLAG_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFP0656127.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVMQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            19563..22160
                     /gene="topA"
                     /locus_tag="ACHLAG_002938"
     CDS             19563..22160
                     /gene="topA"
                     /locus_tag="ACHLAG_002938"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFP0656128.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKVRYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
BASE COUNT         5405 a         5347 c         6059 g         5408 t
ORIGIN      
        1 atattcgtga agtcgggtaa tgagagtgga acggtggcag tgagaatgga acagaaaaaa
       61 atcagatgcg gaaagcggaa atacacagcg ggtgcgatgt gcttcatgct gtttgtatgg
      121 ctggggggtg tggtgacatt ctgtgtgctg gccgctctga taatgaggtg aaaacggaag
      181 agagtttact tctttaattc gaactttctg ataactgaga acttgttaaa tgctcgtggg
      241 tttatatatt taaatcaatt tgttaatgtt aatttcatct ttatcagtca aagtttgttt
      301 tagtatgatg attgtgccca ctgtttattg ttgtccggga gaaaatacag ggaatcccag
      361 ggaatgaaca aaatatactc actcagagtt aatgacagaa aagaactggt tgctgtaccg
      421 gaaactgtgg ggggcaggaa aaagtcgtcc ggcattcccg gtgcggggat gctttcccgt
      481 cttcttctgg cctcaggagc cgttgccggc gttctgtttt cttctcctgc gctggcatcc
      541 gttgtcggga atactctgcc atggcagacc tacagggatt ttgctgaaaa caagggggcg
      601 tttcatgccg gggccacaaa tattccgctt tatggtaaga atggggctgt cagtgggcgg
      661 ctggacaagg ctccgatgat ggatttcagt gttgtggacc agatactggg ggtggcaaca
      721 ctgatttctc cgcagtatgt ggcgggggtt aagcataacg gcagctacaa tacggtgcgt
      781 tttggttatg cggacgatac gacatatcgc cttgttgacc gtaatgagca ctggcgtgat
      841 tttcatactc cccggctgaa caaactggtg acagaagttg ccccggtgag cgtcacggat
      901 gctggaaccg gcaagggggt ataccagaac cgcagtcgtt atccggtgtt ttatcgtatg
      961 gggagtggaa cgcagtacac cggagctgct tccggtgcgc tgaccagaat agcggatgct
     1021 tacgtctgga ggactggcgg gacggtggga agtccggtca tttctgactg gagtctggtg
     1081 tcaaacccgg gaaatcttta ccagggtgtt aatggtccgc tggcgtctta cgggacatca
     1141 ggagacagtg gctcaccgct ttttgcctgg gatgcggtga agaagcagtg ggtatttgtt
     1201 gctgtacttc atggttatgc cggtgagaac gggaagacga actggtttac ggtgattcct
     1261 gcaggggatg tgaataacac gataaagcag gacaattcag gcacagttgt gccggcagtt
     1321 gccggtggtg acattgtgtg gaagtacagt aagggaagcg gagagggaac cctttctcag
     1381 ggcgggcagg tatggaagat gaacggcttc aggggaggga gtctgaatga cgggaaagat
     1441 atcacctttg ggggtaaggg cacggttgtt ctgaaggatg atgttgtcca gggggctggt
     1501 tcgctcacgt ttaatggtga ttatacggtc agaccggaag ggaatcagac ctgggtgggc
     1561 gggggaatca tcgtgaacga cgggcaccgt gttgactgga tggtgaacgg acttgcggga
     1621 gacgcacttc ataaaattgg caagggaata ctggtggtgg ctggcagtgg tgaaaatccc
     1681 ggcgccctga atacggggga cggaacggtc attctggcac agaaggctga tgctgcagga
     1741 aaggtccggg cattcagtga agtcagtatt gtcagcggac gaccagttgt ggtgctgcag
     1801 gacagtcatc agattgaagg ggaccgcatt cgctggggat accggggagg cacgcttgat
     1861 atcaacggaa atgacatggc cttcaaccgc ctggctgcgg ctgatgaggg ggcagtactc
     1921 acaagccggg agcgtcctgc cacagtccgt ctggatttca gccagtcagg gcagaaggcc
     1981 gtgatgtggc atggtcattt tatcggtaac ctcagcgtcc tgaacaacac gtcatctgcg
     2041 gtggatttta tcatggatgg cggagcggat atgtccggca gttttacaca gcagggcggg
     2101 ggactgtata tccaggggca tccggttgtt catgctgtca gcagtgaggc ggttgcgact
     2161 gcattgcgaa agcagggtga taattctgtt ctgacgcagc cggtatcatt tgctcagaag
     2221 gactgggaga gcagaacgtt cagcatcggt caactgaaac tgaagggggc ggcattcagc
     2281 ctttcccgca atgcgacact gacgggagat attgatgctg ataatgccac catggtgctg
     2341 ggaagtgact cgctatatct ggatatgaag gacggcacgg ggagcagcag tgcgccggtt
     2401 aaggggacgt ctgccgtggg cggtgcatcc ggtagcagta catttcgcgg gaatgtgaat
     2461 atgcgtcatt ctgccctgac ggtccgcgac cattttactg gcagtataac ggcatcggac
     2521 agccggatag ctgttagttc agagaatgtg cgtctggaag ggcattcccg cctgacctct
     2581 tctgcactta ctgtcagtga cggcgggcgt ttacatgtga aggggggact ggagacagat
     2641 ggtggtgtga cactggacag aggaatcctt ttggtggacg ggggaagtgt caggaatgat
     2701 gtgtatgaac gtctgctggc ctggtcagag gagcgtggcg ggctgaacgg aagcggtgag
     2761 tatgatttta tgacgggggc agctggtctc ctgcggagtt atgtcagagg gagtgcaggt
     2821 aatgtgaatc tgcagaatgc ggcctggatg atgacgggaa actcatccgt gaagcatctt
     2881 gagtcatcgg gaagtgctct gtatttcagc aggccgggcg gggagttcca taccctgacg
     2941 gcaggcagta tggatatcag cgacagtgtt ctggtgatgc ggacggacct taaccactca
     3001 gaccagcttc gggtgacaga atcgctgcgg ggtaagaata acctgttgtt ggttgatttt
     3061 actgagcgtt ctgacgggca gaaggcgctg aatatccctc tggtgacggc gccggccggt
     3121 accggtgcgg atgtgttcag tgtgaaaacg cgtgataccg gcttcagtca tatcacacct
     3181 gttgtcaggg ctgaacaggg gacgggcggc acggcctggc agctgaatgt tgtgcagccg
     3241 gaaactgccg cagagcctgt ggtgacgcgg caggatgcag agacgccgaa tcctgtggaa
     3301 gctgtatcac cgctcccttc accggtttct gctccgtcac cggctccgga ggcgtcagtg
     3361 acggatgtac tgaccggaga gaatgcgggg gaatcaggtg agtaccgtga tgagacccgg
     3421 tggctgctga caggataccg gagtacggtt aatacctccg cggtcaggga tgcggggatg
     3481 ctgatgtcaa tggggcaccg taacttcatt aatgaggtca acaacctgaa caaacgtatg
     3541 ggtgacctgc gggacatcaa cggcgaggcc ggtgcatggg cccgtatcat gagcggaacc
     3601 ggttctgccg gcggaggctt cagtgacaac tacacgcacg ttcaggtggg tgcggataaa
     3661 aaacatgaac tggacggtct ggacctcttc accggcgtga ccatgacata taccgacagc
     3721 aacgccggca gcgacacctt cagtggtaaa acgaagtcag tgggggcggg tctgtatgct
     3781 tcagccctgt ttgattccgg ggcctatatc gacctgattg gtaagtatgt tcaccatgat
     3841 aatgagtata cggcgacctt tgccggactt ggcacaaaag actacggctc tcattcctgg
     3901 tatgccggtg ctgaagtggg ttaccgctat catgtcacag aggatgcctg gattgagcca
     3961 caggcggaac tggtctatgg tgctgtctcc gggaaacagt tcagctggaa ggaccaggga
     4021 atgaacctca ccatgaagga taaggacttt aatccgctga ttggccgtac cggtgtggat
     4081 gtgggtaaat ctttctccgg taaggactgg aaagtgacgg cacgtgccgg tcttggctac
     4141 cagtttgacc tgctggcgaa cggagaaacg gtactgcgtg atgcatccgg agagaagcgt
     4201 attaagggcg agaaagacgg ccgtatgctg atgtctgtgg gtctgaacgc agaggtccgt
     4261 gacaacatcc gttttggtct ggagtttgag aaatcagcct ttggtaagta caacgttgat
     4321 aatgctgtca acgccagctt ctgttattca ttctgacggt tgttactgaa ggggattatg
     4381 gcctgacggc tccctgtaaa taaagacagg cacagaagat tgtgagttac tcagtgttcc
     4441 accaaaaagt aaggtcgcaa ctctgtgcct gcttcgtaag tgtacagtga gggccatatg
     4501 gttataactg aagcatccga cgacgtggtg tctacccaaa aagcgacctg tgaatattag
     4561 gctggttaag tgcgtatttt catggaagga gatcacccaa taaccccgat ctcctttatg
     4621 ttaatacgca ttaagcgggt aaaacaggaa gattaagaaa gggagagttt ttcgactccc
     4681 caaccaagag aagcagctta taactaccag atagttgtta tgtcatacat gacggataat
     4741 gtcataaaaa atatgtcatt gccgtgatta tgatcgcgtt attggataaa gcagttcagc
     4801 ggtttcccag gcttcctgca gcgtttcctg tacaaattcg tgcgcggatt ctttatttac
     4861 aactcttaaa atggtgagtc catcattgct ggtggttctg acaatcacgt ccacatggtc
     4921 atatccgtta gttatggaat gactataagg ggggataagc ctctgcaaaa caaaaaaaga
     4981 ctttaacaaa atcaatcagt taaaaacaca gaaaaacggt atgaaaaatc accataaaag
     5041 tttgtttttc cttttataaa tagctgttta tcacattttt tataatctac cagtctggta
     5101 catggatatc gataccaccg ccaattataa gctaggcggt gcacagcaac acgatagcgt
     5161 acgcctcgat ccgtgggtgt ttatgttctc agcaggatat cgtttttaat tccacaaaaa
     5221 aacgaccccg taatatacgg ggtcaataag gacatggcat aaagtgtatt atttcttcgc
     5281 ttctacgccg tcagtttcag agcgaattaa aaatttctca gttgtttgcg gaatatgctg
     5341 aatcagccag tcggccattt gcttttcctc atttaaaatc gcttcgatgg ttggaattga
     5401 agctgtatca ccggcatttt ttgctgctgc taatagtgag gtgtaacagg cgatttcaaa
     5461 ttgctcgaag acatatccgc taatagagcc tttgactatt tcatcagaag ggaatatacc
     5521 accgattgac tgcccaagcg cagccatttt actcatggaa tctttaatga ctgaacgtga
     5581 aatgtcatta cgatcaagaa tagtttccaa ttgaacaatc tggtttttgg tttcactaag
     5641 atgttgttca atacgagcgc gtagttcagg ataattatct atacggctgg ccatggattc
     5701 aagcatagat tcggcttgct tttccattgc gtgggcgtca cgtaaccagt catgataatg
     5761 ttcaatacga ttcatgaaaa tgtcctcttt ataaaatgac taaaagttaa agttcatatt
     5821 tcaggcttta ttttcggctt tcttatttac gttattaagg gccagatcag tcagtttgat
     5881 gtcggtggcc ttttcttctt ccagggtctc tttcagaagc ttcgctgctt tacgataacc
     5941 taattgttca gccagcgtcg ctaatgtccc gtaactggca atctcataat gctcgacttt
     6001 ctgtgctgcg gcaatcagtg cggcatcacg cacttcgttt ttctcggtac tttcgatgac
     6061 ctcattagct tcttcaataa gaccttccat tgccacacat ttcatgcgct taattttcag
     6121 attcgattct gattccacaa cttgatcaat acgttcaatc tgtccatgtg tttcctcgag
     6181 gtgcgcatga aaagcctgac ttaatttttc gtttgatgtt gctcttgcga gttttgccag
     6241 tgcccgggtt aattgttttt ctgcgctgta ggtatctgaa agcaggtgaa taaatacatc
     6301 ttcaatggtc ttcatattca tatttttctc cagtgaaatc actgcgagcc atacggcccg
     6361 cagatgcaat tatctttatc agctgatatt aataattaac ggtgaatcag gatttgcctg
     6421 atttattacc accgctttgt tgaccgcctt ttttacccgc ttcagatgcg cgttgcggat
     6481 catttttaaa attaccgccg ctatgctgac cgcctttacg gcctgcgtcg gatgccttct
     6541 cacggtcttc ggcgaaattt cctgaaccac cacgatgttc ggccatgtta tttctcccgt
     6601 tgcgttgcat tgtttcatta atatgagtgt tgtgtgtcga cactcattaa aattagtcgc
     6661 taatgagaat tagtcaaatt aagcgcaacg agaagataga gggaaaatat attttgagga
     6721 acattctgga tatattaaca attacctgag gaataagtga cttagagaaa attattgatt
     6781 ttactggtgt tatgttgcgg gattaatttg tttgattata ttactgttgg gcggaaaaat
     6841 gacgtaagtt gaagttcgac cggggtaagc gaaacggtaa aaagataaat attaaatgaa
     6901 tttaggattt ttccggcttc attaaagaaa gttaaaatgc cgccagcgga actggcggct
     6961 gtgggattaa ctgcgcgtcg ccgctttcat cggttgtaca aaaactttca gtgccgccag
     7021 cattttctct ggctcattaa tatgttgctc gatgatttta acaatggccg aaccagaaat
     7081 cgcgcccgca gctcctgcat caatcgctgc ttttacctga tccggggcgg aaataccaaa
     7141 cccctgcaat ggaggtgcag cgttgtactc tttcagcttc gcaaccagat gattgagggg
     7201 taacgcggcg cggttttctg cgccggtcac gcccgctcgt gacagcaaat aggtgtaacc
     7261 acgaccgtaa gaggctatct ggcgcagcag gtcgtcatcg gcatttggcg ggcagatgaa
     7321 gataggtgcg acattatggc gcaacgcggc ctggcggaag ggcgcggact cttcaattgg
     7381 cacatcggca accagcaccg aatcgacgcc gactttctcg cactgggcat aaaactcatc
     7441 aatgcctttg ttaaacacca gattggcata catcaacagg ccaatgggaa tggtcgggtg
     7501 tttctggcga atcagtgcca gcatttcaaa acattgtgcc ggagtcacac ctgccgcaaa
     7561 ggcgcgcaga gtggcgtttt gaatggtcgg gccatccgcc agtgggtcgg agaaggggat
     7621 acctaactcc agcgcgtcag caccggcttc aattagcgta tcgataattt tcaatgactg
     7681 ctcaatgccc ggatcaccga gcgtgacgaa aggaacgaat gcgccttctt tgcgctcctt
     7741 caactgggca aacagagatt cgtagcgttc catcagattt cccctcgtgc tttcaaaata
     7801 tcgtgaacgg tgaagatgtc tttatcgccg cgaccggaaa ggttaaccac cagtagctgc
     7861 tctttatccg ggttttcgcg catcattttc aacgcatggg ccagggcgtg ggaggattcc
     7921 agcgccggga tgatcccttc gtgcaggcac agcgttttga aggcttcaag ggcttcatca
     7981 tcggtaatag acacgtaatc agcgcgtcca gtgctgttaa gatacgcgtg ttgtgggccg
     8041 acagacggga aatccagtcc ggcggagatg gagtaagatt cttcaatctg cccgtcttcg
     8101 gtttgcatca tcggcgcttt cataccgaaa tagataccca cgcgaccatg ttttagcggt
     8161 gcgccgtgct cgccagtttc gataccgtga ccacctggct ccacaccaat caggccgacg
     8221 ttggtttcat tgatgaaatc agcaaacatg ccgatggcat tcgaaccgcc gccaacacag
     8281 gcgataacgg catccggcag gcgaccttct ctttccagaa tctgcgcttt ggtttcttcg
     8341 ccaatcatcc gctgaaactc acgcacaatg gtcggataag gatgcgggcc agctgcggtg
     8401 cccagcatat agtgcgcggt ttcgtaacta ccggaccagt cgcgcagcgc ctcgttacag
     8461 gcatctttca gcgtcgcgga accgctatgc accgggatca cttccgcacc cattaagcgc
     8521 atacgaaaaa cgttaggcga ctggcgttca acgtctttgg cacccatata aatacggcat
     8581 ttcaggccga gcagggcgct ggcaagggcc gacgccacgc catgctgacc ggcaccggtt
     8641 tcggcgatga tttcggtttt acccatccgc ttcgccagca acgcctgccc cagcacctgg
     8701 ttagttttat gcgcgccgcc gtgcagcaaa tcttcacgct tgagatacag cgtggtgttc
     8761 gtcccggctg taatgttctg gcatttggtc agcgcggttg gacgcccggc atagtttttc
     8821 agcaggtcgt tgaactgagc ctgaaattca ggatcttttt gcgcactgac aaaagcttct
     8881 tccagctggc gcagagcagg catcaggatt tgtggcacgt acatgccgcc aaactcacca
     8941 aaataggggt taagtaatgt tgtcattgtt cctttcctta atatgcgcgc agcgtctgga
     9001 aaaccgaggc caaaagacgt gcgtctttga tgcccggttg cgactctaca gcagaattaa
     9061 aatcaagtcc ggcgcagccg gtttgtgccg cttccacgca gttatctgcg cctaagcccc
     9121 ccgccagcag aacgttgcca agcgattgac catttaatag tgaccagtca aaacgttgcc
     9181 cggttccacc ctggccgttg tctaaaacat atttatcaac gtgctgaaac tcgcgggcgg
     9241 gcagggtttc accgacgctt aatgctttcc agatggcaac atgtgctggc agagcttcac
     9301 gcagcgtatc gatatacagc tgttcttcat taccatgcag ttgcactgcc gccagcgata
     9361 acaccttagc tttgtccacc acatcggcaa tatcgtgatt gcggaacacg ccaacatact
     9421 gcaacggtgc cgcagccatc acttcctgcg cctgttcaac gttgacgcaa cgcggtgatg
     9481 tcgcaacaaa aatcaaccca ccgtaaatcg cgcccgcgtc ataagctgct ttagcatctt
     9541 gcccacgcgt caggccacat actttattct cacccagcaa cacccggcgc acggcggcgt
     9601 gcaaatcgtc atgggccatc aacgccgaac caatcagaaa accgttagcg aagtggctta
     9661 actcgcgcac ctgagcgtaa gtattgatgc cggattcgct gattaccgtc acgttgtgcc
     9721 ccagtttcgg cgcaagctcg cgggtacggt tgagatcaat cgacaaatca cgcagatcgc
     9781 ggttgttgat gccaacgacc tttgccccca atgcaatggc acgctccagt tcctcttcat
     9841 tactgacttc ggtcagcaca cccatctcca gactgtgggc gacggcggca agctggcgat
     9901 attgttcgtc atccagtact gaaagcatta ataagcaggc atcagcctgg taatagcgcg
     9961 ccagatagat ctggtaagga tcgataatga agtctttaca taaaatcggc tgcggggcga
    10021 tttggctgac gatggggagg aaatcaaagc tcccctgaaa atatttctca tcagtcagca
    10081 ccgaaattgc cgaagcgtaa tgtttataaa tggcggcaat gcgtgctgga tcgaaatcat
    10141 cacggatcac gccttttgac ggcgacgctt tcttgcactc cagaataaac gccgtgcgcg
    10201 caccctgtag cgcatcataa aaatgtcgcg tgctcggctg aacctcattc tgaaaactgg
    10261 ccagcggttg ctgctgtttg cgggtttcta cccaaatcgc cttgtctgcg acgattttcg
    10321 ctaaaacggt ttgcatcatt taccctcgtg ccgccagtgc ggtaactctg tcgtaagcgg
    10381 aaccactgcg cagtacctca agaacggttt gcgcattggc ttgcagatct tcatggccat
    10441 gcaggcgcat taacatggcg acgttcgcag cgacggctgc ttcatgggcg gcgtcgcctt
    10501 taccttgtaa caagcgtgtt aaaatgtcac ggttttcttc cggtgttccg cctgccagtt
    10561 gctcctggtg gtagggagtc aggccaaaat cttcagcggt caattgatag ctcttaattt
    10621 cgccgtcatg cagctcggca acgattgtcg gcgcgtgtaa tgaaacttca tccatcccgc
    10681 cgctgtgcac caccgccgcg cgttgatacc ccagcacgtg caaggtttcg gcaatcggca
    10741 gcaccagttc cggactataa acaccaatta acgccagcgg cggatgcgcc gggttaatca
    10801 atggccccag cacattgaac agggtgcggg ttttcagttg ctggcgaacc ggcatcgcgt
    10861 ggcggaatcc ggtgtgatac ttcggcgcaa agaggaaaca tacacctaac tcatccagcg
    10921 cctggcgcga tttatcggcg ttcatatcaa gattaatacc gaacgccgcc agcagatcgg
    10981 acgaaccaga tttactggag acgctacggt tgccgtgttt cgccactttc agcccacagg
    11041 ccgcggcgac aaacgcactg gcggtagaaa tattgatact gttgctgccg tcaccgccag
    11101 taccgacgat atcagcaaac agataatccg ggcgcgggaa cggcgcggcg ttttccagta
    11161 gcgcggttgc tgctccggcg atctcgttcg ggtgctcacc gcgaattttc atgctcacca
    11221 gcgccgccgc cagttgttcc ggcttcagct cgccacgcac caccgctgaa aacagctggt
    11281 ggctttcttg ttggctaagc gtctgcgcct gatacagttt ttccagaatc ggttgcagcg
    11341 tgttggctgg ctctagtttc tgctgcgccc aggccagcgt ttgttccagc aggcgagcgc
    11401 cctgggtggt gagaatggat tccggatgga actggaatcc acaaacgcga tccgcatcgt
    11461 gacgtactgc catcaccatg ccattaaaat gggcgttgat ggttaaaccg gccggaatgt
    11521 tactgccaac cagcgagtga taacgcgcca ccggcagcgg gtttgttaat ccggcaaaca
    11581 tcgcctgacc gtcatgttca atgctcgacg ctttaccgtg aagaatttcg cccgcctgac
    11641 cgacatagcc cccgtaagct tcgacaattg cctgatgtcc gaggcaaatg ccaataatgg
    11701 gcagcttgcc acgcaagcgg gtgaggagtt ccggcataca accggcttcg ctcggcacac
    11761 cggggccagg agaaagcatc agtaccggat tgctcatggt cgccaggcgt tcaattaagg
    11821 tctgcgccgg aatatggttg cggtaaatca ccacgttatg cccattgctg cgcaactgat
    11881 ctgccaggtt gtacgtaaaa gagtcgatat tatcgagcag cagtatgtca gccatcagaa
    11941 agtctcctgt gcatgatgcg cggtggcaat agcgcgcagt acagcgcggg ctttattacg
    12001 ggtttcgtcg gcttccgact gcggaataga atcaaggact acgccagcac cggcttgcac
    12061 ggtggcgata ccgttttcca ccagcgccga gcggatcaca atgcaggtgt cgagatcgcc
    12121 gtgcgcggtg aaataaccta ccgcgccgcc gtagctgccg cggcgacgac cttccgcctc
    12181 ggcaattaac tgcatggcgc gcactttcgg cgcaccgctt aacgtcccca tattcataca
    12241 ggcgcggtaa gcgtgtaggg cgtcgagatc gtggcgcagt tcaccgacca cgcgggagac
    12301 caggtgcatc acgtaagagt aacggtcaac tttggtgagg tcggcgacgt agcggctgcc
    12361 gggggtgcaa atgcgtgcca gatcattacg ggcaagatcc accagcatca gatgttcaga
    12421 aagctcttta tgatcggtac gcatttccag ttcgatgcgg ctgtcgaggt ctctgtccag
    12481 cgagccatcg gcacgacgac cgcgtggacg tgttccggca atcgggtaga tctcaatctg
    12541 gcggctggtg gcgtcatact tgagcgaact ttccggcgac gcgccaaaca gggtgaaatc
    12601 attatcctgc ataaaaaaca tgtacgggct gggattactc tttttcagca cgtaataggc
    12661 cgccagcggt gacgggcagg gcagagagaa acggcgagat ggcaccacct ggaaaatttc
    12721 tccagcgcga atcgcttttt gcaacaaacg cactacgcca ccgaactctt catcgctctg
    12781 attacattca caacgcatat gcggcacgga aaccaccggc agcggcggcg cggcttcggt
    12841 cagttgctga cgtagttcgt tcaggcgagc agtgagacgt tgtttttctt cttcattcgg
    12901 agcaaacagg ctggcctgaa tacgggtgct ttttttctga tggtcaatca ccatcagcgt
    12961 ttcagcgaga taaaaacaga aatcagggca gttattttcc gctgacagtt gcggtaaatc
    13021 ttcaaatccc gccacaaggt cataagagaa caggccgccg aagaacattg cttctcgttc
    13081 ttccttcggt acattcaaca gattctgcaa taaacggaaa gcgtcaaaaa ccgaaaggga
    13141 gcataagcga gcgtcttcat ccagcagtgg actgacaggg gggaagcgca gcacacggca
    13201 gtttggtaat tgttcatttt ccacacccgc aggcagggcg ttatccagta gtgtcagcag
    13261 ggcttcgccg ttgccggaaa gtgcctggat tgtgacagtg tcacctaaag ctgtaatgcg
    13321 cagcgcactg tctaccagca gcaggctttt taaatcatct ttgctgtcga tatctgcgga
    13381 ttccagcagc agcgttgccg gacgatcccc acacaactgg tgaaaaagcg cggtgggatt
    13441 gtcgcgataa gcgccttcgc aggttagcag ttcgagagtc ggtttttgtg tttgcattgt
    13501 tattctctaa ttttgttcaa aaaaaagccc gctcattagg cgggctgggt atctgattgc
    13561 tttacgcatg gtgaatacac tgcccgtttc aggaagtgcg ccaccaacct ttcagtacga
    13621 aaattgcttt cattgtcgat acccttttta cgtgaacttg cgtactagtt aactagtttg
    13681 atgattaatt gtcaacagct catttcagaa tatttgccag aaccgttatg atgtcggcgc
    13741 aaaaaacatt atccagaacg ggagtgcgcc ttgagcgaca cgaattatgc agtgatttac
    13801 gacctgcaca gccataccac agcttccgat ggctgcctga cgccagaagc attggtgcac
    13861 cgtgcagtcg agatgcgcgt cggcaccctg gcgatcaccg accatgacac cacagctgca
    13921 atcgcgccag caagagagga aatttcacgt tcgggactgg cgttgaatct tattcccggc
    13981 gtggaaattt ccacggtctg ggaaaatcat gaaattcata ttgtcgggct gaatattgat
    14041 attactcatc cgctaatgtg tgagttcctc gcgcagcaga cagaacggcg caatcagcgg
    14101 gcgcagctga ttgccgaaag acttgaaaaa gcgcaaatac ctggggcgct ggaaggcgca
    14161 caacgactgg cgcagggcgg cgcagtgacg cgcggtcatt ttgcgcgttt ccttgttgag
    14221 tgcggcaaag ccagttcaat ggcggatgtc tttaaaaagt atctggcgcg cgggaaaacc
    14281 ggatacgttc cgccacagtg gtgtacaata gaacaagcta ttgatgtcat tcatcattct
    14341 ggcggtaagg cggtgctagc tcatcctggg cggtacaatc ttagtgctaa atggctgaaa
    14401 agattggttg cgcattttgc cgaacaccac ggtgacgcga tggaagtcgc gcagtgccag
    14461 caatcgccca atgaacgtac ccagctggcg gcccttgcgc gtcagcatca tttatgggca
    14521 tcacaaggat ctgattttca tcagccatgc ccgtggatcg aactgggtcg taaactctgg
    14581 ctgcccgcag gcgttgaagg cgtctggcag ctatgggaac agccgcagaa caccacagag
    14641 agggaattat gagccagttt ttttatattc atcctgataa cccacagcaa cgtctgatca
    14701 accaggcggt ggagatcgtg cgtaaaggcg gggtgattgt ttatccaact gattccggct
    14761 atgcgctcgg ctgtaaaatt gaagataaaa acgcgatgga gcgtatttgt cgtattcgcc
    14821 agctgccgga cggtcacaac tttaccctga tgtgtcgcga tctttctgaa ctctcgacct
    14881 attcatttgt tgataacgtt gcgtttcgtt taatgaaaaa caacacgccg ggcaactata
    14941 ccttcatctt gaaggggacg aaggaagtgc cacgccgtct gttgcaggaa aaacgcaaaa
    15001 ccatcggtat gcgtgtgcct tctaacccga ttgcgcaggc gttgcttgaa gcactgggcg
    15061 aaccgatgct ttccacttcg ctaatgctgc caggcagcga atttaccgaa tccgatcccg
    15121 aagaaattaa agaccgtctg gaaaaacagg tggatttgat tatccacggc ggctatctcg
    15181 gacagaaacc gacaacggtt attgatctta ccgatgatac gccggtcgtg gtgcgtgaag
    15241 gcgtaggtga tgtgaagcct ttcttataag tgcgtaactc tgtatactac gcggccttaa
    15301 aatggcgcta tacggcgcca ttgattctcc tgttcgacgc ctgtgaaggc gacaccaaaa
    15361 ggaagctcta tgagcgaaaa attacagaaa gtgctggccc gcgctggcca cggctcccgc
    15421 cgtgaaatcg aatctattat tgaagccggt cgtgtgagtg ttgatggcaa aattgctaaa
    15481 ctcggcgatc gcgttgaagt tacccctggg ctgaaaattc gtatcgatgg tcatctgatt
    15541 tcggtacgtg agtccgctga acaaatttgt cgcgttctgg cctattacaa accggaaggt
    15601 gagttgtgta cccgtaacga tccggaaggg cgtccgacgg tgtttgaccg tctgccaaag
    15661 ctgcgtggcg cacgctggat tgccgtaggt cgtctggacg ttaacacctg tggtctgctg
    15721 ctgttcacca ccgatggtga actggcaaac cgtttaatgc atccaagccg tgaagttgaa
    15781 cgtgaatacg ccgtgcgtgt atttggtcag gttgacgacg cgaaactgcg tgatttgagt
    15841 cgtggcgtgc aactggaaga tggtccggca gcttttaaaa ccatcaagtt cagcggcggc
    15901 gaagggatca accagtggta caacgtgact ctgaccgaag gccgtaaccg tgaagttcgt
    15961 cgtctgtggg aagcggttgg tgtgcaggtg agccgcctga tccgtgttcg ttacggtgat
    16021 atcccactgc caaaaggtct gccacgcggt ggctggacgg agctggacct cgcccagact
    16081 aactatctgc gcgaactggt ggagcttccg ccagaaacca gctctaaagt cgctgtagaa
    16141 aaagaccgtc gtcgcatgaa ggctaatcag attcgtcgtg cggtgaaacg tcacagtcag
    16201 gtgagcggcg gtcgtcgttc tggcgggcgt aataacggtt aatcaaagta tgccggacgt
    16261 catatccggc atttttacag attaataatc aatccctatc tgcgctttta caccggcatc
    16321 aaacgcatgt ttgacggggc gtaattcact taccgtatct gccagttcaa gaatatcccg
    16381 atgacaacca cgacccgtga taatcaccgt ctgttgatgt ggacgttcat ttaacgcctg
    16441 caccacttct tccagtggca aatagtcata cgccaccata tacgtcagtt catcaagcaa
    16501 aaccatatcc agtgaggaat cagcaagcat ccgctttgca tgttgccaga cttcgcggca
    16561 ggcggcggta tcagactcgc ggttttgtgt atcccaggta aagcccgttg ccatcacctg
    16621 aaactcaacg ccatgtggct ccagcagatt gcgttcgcca ttaggccagg tgcctttaat
    16681 aaactgcacg acgcctactt tttttccgtg accaactgcg cgtgttgccg taccaaatgc
    16741 cgcggtggtt ttgccttttc cattgccggt aaagacgatg ataataccgc gttcatcctg
    16801 ggcctgggcc acacgagcat ctactttttc tttcactcgc tgctgacgct gttggtagcg
    16861 ttcatcactc attgggaaat tcctggttta cggcccggtt gggcgtcaaa ggtcatgccg
    16921 gttttacggc ggctgtcatc gcccatcagc cagaggtaga gcggcatgat atcagcgggt
    16981 gttttaagtt tctgtggatc ttcggtcggg aaggcgcttg cgcgcattgc ggtgcgcgta
    17041 ccgcctgggt taatgcagtt gacgcgcagg cgctgctgat attcatcggc cagtacctgc
    17101 atcatccctt cggtggcaaa tttcgacgct gcatatgcac cccagttagc tcgtccctga
    17161 cgtccaacgc ttgatgaagt aaagaccagt gaaccggcgt ccgatttgag taataaagga
    17221 agcagtgcct gggtgagcat aaaggtggca ttaacgttga cctgcatgac gtcctgccag
    17281 acctgcggat tttgttcgct cattgggcaa atatcgccga gcaatccggc attatgcaaa
    17341 acaccatcca gacgcggata attaacgaca atgcgctgtg ccagttgttg gcaattttcg
    17401 gacgtgcagg tcagcaaatc gagaataaac cactgtggct gacgcccagt ttcttcgttt
    17461 atgtggctgg ctacctgacg taatttttct tcattacggc ccaacagaat cactgtcgca
    17521 ccatagcgtg catacgtcat cgcggcttca cgaccaatcc catcgctggc tcccgtcacc
    17581 aggataatgc gatcattgag taaatcttgt tttggctggt aatgcatggc tactcctcaa
    17641 cgacgttgtc tgtcgcgctg ccagtgtact ttatgacttt ggggctttat gcctgaaaca
    17701 gaagggcatt tcaatcagcc tggggcgata acaccgcaaa atggatactt tgtcatactt
    17761 tcgctgcaat aacatctctg cgagacggct taacatgcct gttgtaaact gtgagccaaa
    17821 gcgttgttta accaaggtgg ggactcgtgg aattgttgtc tgaatatggt ttgtttttgg
    17881 cgaaaatcgt taccgttgtg ctagcgattg cggcgattgc cgccattatt gtcaatgttg
    17941 ctcaacgtaa taaacgccag cgtggcgagt tacgggtcaa caatctcagc gaacagtata
    18001 aggagatgaa agaagaactg gccgcggcgc tgatggactc acatcagcaa aaacagtggc
    18061 acaaagcgca gaagaaaaag cacaagcaag aagcgaaagc agcaaaagcg aaagccaaac
    18121 tgggcgaggt ggcaactgac agtaaacctc gcgtctgggt gctggatttt aaaggcagca
    18181 tggacgccca tgaagtgaac tcgctacgtg aagagataac ggctgtactc gcagcattca
    18241 aaccgcagga tcaggttgtg ctacgtctgg aaagccctgg tggcatggtg catggttacg
    18301 ggttggcggc ttcgcagctg cagcgtctgc gtgataaaaa cattccttta actgttacgg
    18361 tagacaaagt cgctgccagc ggcggttaca tgatggcctg tgtggcggac aaaattgttt
    18421 ccgcaccgtt tgctattgtg ggttccattg gcgtggtggc gcaaatgccc aactttaacc
    18481 gcttcctgaa aagcaaagat attgatatcg aactgcacac tgccgggcag tataagcgta
    18541 ctctgacctt gctgggtgaa aataccgaag aagggcggga gaaattccgc gaagagttga
    18601 acgaaacgca tcagttgttt aaagattttg tgaagcgtat gcgtccgtct ctggatattg
    18661 aacaggtggc aacgggtgaa cactggtacg gacaacaggc ggtagagaaa ggcctggttg
    18721 atgaaatcaa caccagtgat gaagttattc ttagcctgat ggaaggccgt gaagtggtta
    18781 atgtacgcta tatgcagcgt aaacgactca ttgaccgatt caccggcagc gcggcagaga
    18841 gcgccgatcg attgttgcta cgctggtggc agcggggtca aaagccattg atgtaaaaga
    18901 caaacgcgag gctaagacct cgcgttttgc tttaatcaac cagatgatat ttttctgaaa
    18961 gcacatgggc caggtgtttg aacatattaa acaccgcggt gcttttggct gttggcaatc
    19021 cttgttcatc taaaaagtag tcgccggtaa ataccagcac gccattacgc tgcatgacgc
    19081 cggtggcttc aatccctgcg agcgtatcct catgctcacg aatgattttg ttggcctctt
    19141 tcaacagcgt ttcgcggtcg atgggttgtg tctctttgtt cattatttac tccttaaaca
    19201 aggacattag tctacgccag gcatggcttg cagacaaata taccacgctg gtggcaagag
    19261 cgccttactg gtaactttgg attttgcatg ctaataaagt tgcgtatcgg attttatcag
    19321 gtacagtgtg acgctttcgt caatctggca atagatttgc ttgacattcg accaaaattc
    19381 cgtcgtgcta tagcgcctgt aggccaagac ctgttaactc agtcacctga attttcgtga
    19441 acagagtcac gacaaggggt tgatatccgc agagaacgag tccatatcgg taactcgttg
    19501 ccagtggaag gtttatcaac gtgcgacgca ttcctggaag aatcaaatta ggtaaggtga
    19561 atatgggtaa agctcttgtc atcgttgagt ccccggcaaa agccaaaacg atcaacaagt
    19621 atctgggtag tgactacgtg gtgaaatcca gcgtcggtca catccgcgat ttgccgacca
    19681 gtggctcagc tgccaaaaag agtgccgact ctacctccac caagacggct aaaaagccta
    19741 aaaaggatga acgtggcgct ctcgtcaacc gtatgggggt tgacccgtgg cacaattggg
    19801 aggcgcacta tgaagtgttg cctggtaaag agaaggtcgt ctctgaactg aaacaactgg
    19861 ctgaaaaagc cgaccacatc tatctcgcaa ccgaccttga ccgcgaaggg gaagccattg
    19921 catggcacct gcgggaagtg attgggggtg atgatgcgcg ctatagccga gtggtgttta
    19981 acgaaattac taaaaacgcg atccgccagg catttaacaa accgggtgag ctgaatattg
    20041 atcgtgttaa tgcccagcag gcgcgtcgct ttatggaccg cgtggttggg tatatggttt
    20101 cgccgctgct atggaaaaag atcgctcgtg gtctgtctgc cggtcgtgtg cagtcggtgg
    20161 cggttcgcct ggtggtcgag cgtgagcgtg aaattaaagc gttcgtgccg gaagagttct
    20221 gggaagtcga tgccagcacg accacgccat ctggtgaagc gttggcgtta caggtgactc
    20281 atcagaacga caaaccgttc cgtccggtca acaaagaaca aactcaggct gcggtaagtc
    20341 tgctggaaaa agtgcgctac agcgtgctgg aacgtgaaga caaaccgaca accagtaaac
    20401 ctggcgctcc ttttattacc tctacgctgc aacaagctgc cagcacccgt cttggatttg
    20461 gcgtgaaaaa aaccatgatg atggcgcagc gtttgtatga agcaggctat atcacttaca
    20521 tgcgtaccga ctccactaac ctgagtcagg acgcggtaaa tatggttcgc ggttatatca
    20581 gcgataattt tggtaagaaa tatctgccgg aaagtccgaa tcagtacgcc agcaaagaaa
    20641 actcacagga agcgcacgaa gcgattcgtc cttctgacgt caatgtgatg gcggaatcgc
    20701 tgaaggatat ggaagcagat gcgcagaaac tgtaccagtt aatctggcgt cagttcgtag
    20761 cctgccagat gaccccagcg aaatatgact ccacgacgct gaccgttggt gcgggcgatt
    20821 tccgcctgaa agcacgcggt cgtattttgc gttttgatgg ctggacaaaa gtgatgcctg
    20881 cgttgcgtaa aggcgatgaa gatcgcatct taccagcagt taataaaggc gatgctctga
    20941 cgctcgttga acttacacca gcccagcact ttaccaagcc gccagcccgt ttcagtgaag
    21001 catcgctggt taaagagctg gaaaaacgtg gtatcggtcg tccgtctacc tatgcgtcga
    21061 tcatttcgac cattcaggat cgtggctacg tgcgagtaga aaatcgtcgt ttctatgcgg
    21121 aaaaaatggg cgaaatcgtc accgatcgcc ttgaagaaaa tttccgcgag ttaatgaact
    21181 acgactttac cgcgcagatg gaaaacagcc tcgaccaggt ggcaaatcac gaagcagagt
    21241 ggaaagctgt actggatcac ttcttctcgg atttcaccca gcagttagat aaagctgaaa
    21301 aagatccgga agagggtggt atgcgcccga accagatggt tctgaccagc attgactgcc
    21361 cgacttgtgg tcgcaaaatg gggattcgca cagcgagcac cggggtattc cttggctgtt
    21421 ctggctatgc gctgccgccg aaagagcgtt gcaaaaccac cattaacctg gtgccggaaa
    21481 acgaagtgct gaacgtactg gaaggcgaag acgctgaaac caacgcgctg cgcgcaaaac
    21541 gtcgttgccc gaaatgcggc acggcgatgg acagctatct catcgatccg aaacgtaagt
    21601 tgcatgtctg tggtaataac ccaacctgcg acggttacga gatcgaagag ggcgaattcc
    21661 gcattaaagg ttatgacggc ccgatcgttg agtgtgaaaa atgtggctct gaaatgcacc
    21721 tgaaaatggg gcgtttcggt aaatacatgg cctgcaccaa cgaagagtgt aaaaacacac
    21781 gtaagatttt acgtaacggc gaagtggcac caccgaaaga agatccggtg ccattacctg
    21841 agctgccgtg cgaaaaatca gatgcttatt tcgtgctgcg tgacggtgct gccggtgtgt
    21901 tcctggctgc caacactttc ccgaaatcgc gtgaaacgcg tgcgccactg gtggaagagc
    21961 tttatcgctt ccgcgaccgt ctgccggaaa aactgcgtta tctggccgat gcgccacagc
    22021 aggatccgga aggtaataag accatggttc gctttagccg taaaaccaaa cagcaatatg
    22081 tctcttcgga aaaagacgga aaggcgactg gctggtcagc attttatgtt gatggcaaat
    22141 gggttgaagg aaaaaaataa cctttaattc tgtcaggttt ttataaacaa agggtcgcga
    22201 aagcggccct tttttattg
//
LOCUS       DAZGVQ010000080        21990 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000080 DAZGVQ010000000
VERSION     DAZGVQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.081"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            79..426
                     /locus_tag="ACHLAG_002939"
     CDS             79..426
                     /locus_tag="ACHLAG_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFP0656129.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLCMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(446..1036)
                     /locus_tag="ACHLAG_002940"
     CDS             complement(446..1036)
                     /locus_tag="ACHLAG_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656130.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDLERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVQLNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRLEGDPLEVAQWLQACHDAGMEVRVQVNESVTP"
     gene            complement(1036..1293)
                     /locus_tag="ACHLAG_002941"
     CDS             complement(1036..1293)
                     /locus_tag="ACHLAG_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656131.1"
                     /translation="MDEERVFSLSYEQLTRFAEKRIRECNLDSQGAIYLCESAKAGAV
                     LIFWHELAINGYASMNAIKRQELIEADFQRLRKLIWPEDDR"
     gene            1862..2275
                     /locus_tag="ACHLAG_002942"
     CDS             1862..2275
                     /locus_tag="ACHLAG_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656132.1"
                     /translation="MFFIENEGQAVARTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGTLNGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVRTHGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            2277..3059
                     /locus_tag="ACHLAG_002943"
     CDS             2277..3059
                     /locus_tag="ACHLAG_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP0656133.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQR
                     TEKAARTAGRMPAGQQTHRLVPLSDSWHVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEVYTKVFALDVAARHRVQFLMPESDAVTMLKNRHA"
     gene            complement(3190..3465)
                     /locus_tag="ACHLAG_002944"
     CDS             complement(3190..3465)
                     /locus_tag="ACHLAG_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP0656134.1"
                     /translation="MVKKPSQQALNRAAVTVEQAEALAQRLADKPYGAPEKTEPEKQC
                     RTTISLGESMLVTIEDLALRNKRNGKDPKNVSAIVRVALEQYLKTLT"
     gene            complement(3459..4103)
                     /locus_tag="ACHLAG_002945"
     CDS             complement(3459..4103)
                     /locus_tag="ACHLAG_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0656135.1"
                     /translation="MITVVGGNKGGSGKTTIASNLAIALANKGREVCLLNGDLQRTAA
                     KHHAEREAAGLLPAITLVEKFDNLTQTLQALNEKFDDVIVDVAGRNSKEFITSGVVAH
                     QIIAPLQCSQPDLDTLTELEQQIDAMRNLNPELKVYCLQSMATTNPVLRGNERKEFLE
                     YLEEFPTIQVLDSVICFRKVYRDCMSNGTGVVETNNTAAKAEIEHLMNEVFGPW"
     gene            4332..5303
                     /gene="parM"
                     /locus_tag="ACHLAG_002946"
     CDS             4332..5303
                     /gene="parM"
                     /locus_tag="ACHLAG_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /protein_id="HFP0656136.1"
                     /translation="MNVYCDDGSTTIKLAWNDNGKICKSLSQNSFRHGWKVDGLGIRQ
                     TFNYELDGKKYTYDEVSNQSILTTHIEYQYTDVNLLAVHHALLNSGLAPQPVSLTVTL
                     PISEFYTKECQKNELNIQRKIENLMRPIRLNKGDVFTIEHVDVMPESLPAVFSRLVMD
                     KVGQFEKSLVVDIGGTTLDVGVIVGQFDSVSAIHGNSGIGVSSVTKAAMSALRMASSD
                     TSFLVADELIKRRNDPDFVRQVINDETKTDLVLNTIEGAIASLGEQVVNELGDFHHVN
                     RVYVVGGGAPLIYDSIKTAWHHLGQKVVMMESPQTALVEAIAAFKEE"
     gene            5308..5700
                     /locus_tag="ACHLAG_002947"
     CDS             5308..5700
                     /locus_tag="ACHLAG_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFP0656137.1"
                     /translation="MDDERKRKKYTLYLHPEKAADFQTLEAIESVPRSERGELFRNAF
                     ISGMALHQLDPRLPVLLTAILSEEFSADQVVTLLSQTTGWKPSQADIRAVLTELGASQ
                     SAEKMPPSATDSVQKAMNDVRLKMKKLF"
     gene            complement(5705..6976)
                     /locus_tag="ACHLAG_002948"
     CDS             complement(5705..6976)
                     /locus_tag="ACHLAG_002948"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFP0656138.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEVFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLSLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKQEMLSPSYTTQWTEIPIASF"
     gene            complement(6976..7413)
                     /gene="umuD"
                     /locus_tag="ACHLAG_002949"
     CDS             complement(6976..7413)
                     /gene="umuD"
                     /locus_tag="ACHLAG_002949"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFP0656139.1"
                     /translation="MSTVYHRPAAPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRGEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIRRTRELR"
     gene            complement(7410..7658)
                     /locus_tag="ACHLAG_002950"
     CDS             complement(7410..7658)
                     /locus_tag="ACHLAG_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP0656140.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            8076..8978
                     /locus_tag="ACHLAG_002951"
     CDS             8076..8978
                     /locus_tag="ACHLAG_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFP0656141.1"
                     /translation="MYFSAEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKNMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGDNFIQVDF
                     DTPWCQPESDVVAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVAHYGG"
     gene            8975..9286
                     /locus_tag="ACHLAG_002952"
     CDS             8975..9286
                     /locus_tag="ACHLAG_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656142.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRSLWSVSRV
                     RRARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGITLPSNRRRLRYSTVGLTRYRT
                     R"
     gene            9362..9433
                     /locus_tag="ACHLAG_002953"
                     /pseudo
     CDS             9362..9433
                     /locus_tag="ACHLAG_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9515..11215)
                     /gene="ltrA"
                     /locus_tag="ACHLAG_002954"
     CDS             complement(9515..11215)
                     /gene="ltrA"
                     /locus_tag="ACHLAG_002954"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP0656143.1"
                     /translation="MTAKNINAGAPTDIVTHWHNINWARCRREVRRLQARIVKAIQEE
                     KAGKVKALQWLLTHSFSARAMAVKRVTDNRGKRTPGVDGKTWSKPGSKMKAIYTLKRR
                     GYKPLPLRRIYIPKSNGKKRPLGIPTMKDRAMQALYLMALEPVAETTADPNSFGFRPC
                     RSTADAIEQCFTTLHRADRAQWILEADIRSCFDEISHEWLIANIPTDTAILKRWLKAG
                     YIDLGKLYPTSAGTPQGGIISPTLANMVLDGLQPLLKKTFYRGGLNPEKINIIRYADD
                     FVITGISHDTLSEKVLPLLENFLAERGLTLSPEKTRITHISDGFDFLGMNIRKYNGKL
                     LIKPSPQKVKEFLKRVRQTIKDNPTVSQSTLIWHLNPVLKGWARYYRHVVSKKIFQKV
                     SHEIWKALWRWACRRHPKKGTGWIRKKYFRSVNFRHWVFGTETGKLLPSGKRELLALY
                     EISDMPVKRHRQIKSTANPFDPSYESYFEARLKATMLESIQGYSRLRNLWLRQGGQCP
                     VCHLPVNATTGWQLRHIVPKSEGGLDNTGNLAMLHPACHHTAKHQGISVMKPALMWSY
                     "
     gene            11837..12448
                     /locus_tag="ACHLAG_002955"
                     /pseudo
     CDS             11837..12448
                     /locus_tag="ACHLAG_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
     gene            12449..12670
                     /locus_tag="ACHLAG_002956"
     CDS             12449..12670
                     /locus_tag="ACHLAG_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656144.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            12630..13116
                     /locus_tag="ACHLAG_002957"
                     /pseudo
     CDS             12630..13116
                     /locus_tag="ACHLAG_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            13162..13938
                     /locus_tag="ACHLAG_002958"
     CDS             13162..13938
                     /locus_tag="ACHLAG_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656145.1"
                     /translation="MNENTTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     FFPYAGEWLTEDEIRAVLDAVRDAVRCVSYQVADDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDYPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVSECVEHILASGLVCDV
                     LRIPDEPSRRWFDREILREVVLEARDEIRSMADALAKIRK"
     gene            14353..14778
                     /locus_tag="ACHLAG_002959"
     CDS             14353..14778
                     /locus_tag="ACHLAG_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0656146.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            14825..15244
                     /locus_tag="ACHLAG_002960"
     CDS             14825..15244
                     /locus_tag="ACHLAG_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFP0656147.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEHGADVSIVHDVVRELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
     gene            15244..15435
                     /locus_tag="ACHLAG_002961"
     CDS             15244..15435
                     /locus_tag="ACHLAG_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656148.1"
                     /translation="MNISKETREILHNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRHR"
     gene            15398..15751
                     /locus_tag="ACHLAG_002962"
     CDS             15398..15751
                     /locus_tag="ACHLAG_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656149.1"
                     /translation="MVTTSFRAAGTGDTGQAVEAACQPARKPLAGWRLPEGMRATLAR
                     RPLPFLLVTGVVSGSTCYRPVVKEPSYTCAPSAPGCLAASGRQARLRRAAAGAPASLR
                     RTRWRGFGLPLRSDA"
     gene            complement(15751..15819)
                     /locus_tag="ACHLAG_002963"
                     /pseudo
     CDS             complement(15751..15819)
                     /locus_tag="ACHLAG_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
     gene            16188..16715
                     /locus_tag="ACHLAG_002964"
     CDS             16188..16715
                     /locus_tag="ACHLAG_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0656150.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGDDYGFSDDIPF"
     gene            16773..17006
                     /locus_tag="ACHLAG_002965"
     CDS             16773..17006
                     /locus_tag="ACHLAG_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFP0656151.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDGAGYWMNQYIRDFGILK"
     gene            17065..19023
                     /locus_tag="ACHLAG_002966"
     CDS             17065..19023
                     /locus_tag="ACHLAG_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFP0656152.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDATDDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFDSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFLALMAEKARL
                     AALLPEGWSRDMTKFLSLSQEVLLSLLSFCTACSLNGVQTRECGRTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCSPRPQTDATERADNLADAA"
     gene            19078..19512
                     /gene="psiB"
                     /locus_tag="ACHLAG_002967"
     CDS             19078..19512
                     /gene="psiB"
                     /locus_tag="ACHLAG_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFP0656153.1"
                     /translation="MNTELTLNALQSMNAQEYEEIRAAGSDMRRNLTHEVMRKVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPAAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            19509..20228
                     /locus_tag="ACHLAG_002968"
     CDS             19509..20228
                     /locus_tag="ACHLAG_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFP0656154.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            20225..20821
                     /locus_tag="ACHLAG_002969"
     CDS             20225..20821
                     /locus_tag="ACHLAG_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656155.1"
                     /translation="MMKSDEQYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLAWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVSITDVLR"
     gene            21286..21786
                     /locus_tag="ACHLAG_002970"
     CDS             21286..21786
                     /locus_tag="ACHLAG_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFP0656156.1"
                     /translation="MSVVAPAVYVGTWHKYNGGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
BASE COUNT         4735 a         5722 c         6196 g         5337 t
ORIGIN      
        1 aacataacca catcatgata ataataatca ataacaataa gctgtgtcac gtttacatca
       61 tggaacggag gtgctcctgt gacctttgag cagaaaaaag cccgggccat tgccctgatg
      121 gacagcaaaa agatgtggcg gagcaattat gcccctcctc ttctgcgtat tctctggcgg
      181 ctgggtatcc ggcttccgcc attacccttt atgccgttct ggcaggtcac ggtgctgacg
      241 gggggtttat ggggcatttc ctggggatgt gcaatgtggt ttatctactg ggggccgtca
      301 ggaatggtcg caggtgaggc cattattata agtatcacgg gtggtttttt gttcggactg
      361 tgcatggcct ctttccactg gtggcgcagg aaagttaacc ggctgccacc atgggatgat
      421 gtgtaaaccg tgcacaggcg tgtttttacg gagtgacgct ttcattaacc tgtacacgaa
      481 cctccattcc ggcatcgtga caggcctgca gccactgcgc cacttccagc ggatcgccct
      541 ccaggcgtac caccctgcct tctttattcc ataactgcag acaggtgctg ccgtcgagac
      601 gtaccacaaa atccccacgg caggcctgat acgttatgcc gttcagctgc accgccagat
      661 cagtggcaga agtgccgtct gcgggcagac ctgctgccgg gaccagcggc agtacgacga
      721 cttctgccgc acgctgttct gcccggaggc ggtcctgttc tgccagcagc agaggctgtg
      781 ccacggcccg gccggcgtcc gtcagctcca ctgccagctg caggttcgga gcgcgcaggg
      841 tacgcagcca gccggcatcc tccagacgac ggcaggaggc gcgcaggttt ggcccgtaga
      901 ccggggcttc cccgcttcgc tccagcaccc gttccaggtc ccgcgtcagc acggggcccg
      961 gccgtttttc atcaagggca gccagcacaa tcagcactct ccgctgcagt ggcgacggtc
     1021 ggcggctctg ggtcattacc tgtcatcctc cggccagatg agctttctga ggcgctgaaa
     1081 gtcggcttca atgagctcct gtctctttat ggcattcatt gaggcatagc catttatggc
     1141 cagttcatgc cagaagatca gcacggctcc ggctttggct gactcgcaga gataaatcgc
     1201 gccctggctg tccagattgc attcccggat gcgtttttcc gcaaaccggg tcagttgttc
     1261 atagctgagt gaaaacaccc tttcttcatc catcctgcac actccatctg ataatgtcct
     1321 ttaagaaata aatcatatct ggaattattt atttctatat ctgtaatcat catgtcagat
     1381 atgatgattt gatttatgat aaatatcata tcagatgtga tctcatatgt tttatctgat
     1441 aatcatatct gttgccggtt ttccgccgtg cagatccggt ttccggctgt tcaggatctt
     1501 cctctccccc cacataacgc cacctcctgt cagcacaata tgtagtgctg aattcagctg
     1561 ctgatgacac tatatgttgt gtcatctccc tgacctgtga tgcgccgcgc aggggcggaa
     1621 aacagcgata tgattatttt ctcagcgtgg tacacttccg gaaagttgtg atattccgga
     1681 aagtcggatc tgacggaaac ggctctccgg taatttaacg gcgtggttat atggatgctt
     1741 gttatcatgg tgatgatgat aacggcatga tgttatcaga cggcgtgacg gtaagggcag
     1801 tgatgatgga tgacgttatc gcatgaccgt ccctgcccgg aaaagaaaaa aggagtcacc
     1861 catgtttttt attgagaatg aaggtcaggc tgtcgccaga acggattact ggcagtctgt
     1921 acaggcgcag gccggatatg tctacctcag ctggaatgcc ggcgcagcca ggctgcttgt
     1981 cccggatgcg gcaaaacatt tactcaggga gatgcggggg gctgagtacg tcatcatcag
     2041 taagggaacg ctgaatggcc gcgatgcgct ggaactggta tttgaagacg gcagcgatgc
     2101 gccgtttgtg atccacatgc tgagtgagca gtgcgatcgc ctgctccccg aaaacaacca
     2161 gggagggggt tttgttgtca ccgtccggac gcatggcggt aaccagctcc gttatccggg
     2221 aaagtaccgt gttgtggaaa acctgcccga cgtttccccg tggagtgaac actgatatgc
     2281 agcacctgcc ggcaccgatc caccatgccc gggatgctgc tcagcttcct gttgccatcg
     2341 attatccggc agcgctggca ctccgccaga tgtcgatggt tcatgatgaa ctgccaaaat
     2401 acctgctggc ccctgaagtg agcgccctgc tccattacgt cccggatctg cgccgcaaga
     2461 tgctgctggc cacactgtgg aacaccggtg cgcgcattaa tgaagcactg gcgctgacgc
     2521 ggggggattt ttcgcttgcg cccccgtatc cgtttgtgca actggccact ctgaagcagc
     2581 ggacagaaaa agccgccagg acggcaggaa gaatgcccgc cggtcagcag actcaccggc
     2641 tggttccgct ctccgactcc tggcacgtca gccagctgca gacgatggtg gcaacactga
     2701 aaatccccat ggaacggcgt aataaacgaa caggcaggac agagaaagcg cggatctggg
     2761 aagtgactga cagaacggtc aggacctgga ttggggaggc ggttgccgcc gctgccgctg
     2821 atggtgtgac gttctctgtc ccggtcacgc cacatacgtt ccgccattcc tatgcgatgc
     2881 acatgctgta tgccggtata ccgctgaagg tcctgcagag cctgatgggg cataagtcca
     2941 tcagctcaac ggaggtctac acgaaggtgt ttgcactgga tgtggctgca cggcaccggg
     3001 tgcagttttt gatgcctgag tccgatgcgg tcacaatgct gaaaaacaga cacgcataat
     3061 tcacaaatca taattatgaa ttattctgta ataaaagaga ccactgcaat atgtgatctc
     3121 ttgtatgcaa gggtgcttaa acagtatgaa ttcacaagtg tgatttcata agtaataact
     3181 tcttgattat tacgttagtg tttttaagta ttgctcaagg gctactctga cgatggcact
     3241 tacatttttt ggatctttac cattgcgttt attcctcaat gccagatctt ctattgtgac
     3301 aagcatgctt tcaccaagag agattgtcgt ccgacattgt ttttctggtt cggttttttc
     3361 cggcgcgccg tatggtttat ccgcaaggcg ctgagccaga gcctctgctt gttctaccgt
     3421 aactgcagct ctgtttaatg cttgctggct gggttttttc accatgggcc aaatacctca
     3481 ttcattaaat gttcaatttc tgctttggct gctgtattgt tcgtttctac tacaccggtg
     3541 ccgttgctca tgcaatcgcg atagactttt ctgaaacaaa taactgaatc caggacttga
     3601 attgttggga attcttcaag atattcaagg aattcttttc tctcatttcc cctcaaaacc
     3661 ggattggttg ttgccatact ttgtaaacaa taaaccttaa gttccgggtt aaggttacgc
     3721 attgcgtcta tctgttgttc tagttctgta agagtatcca gatctggttg agaacactgt
     3781 agtggcgcaa ttatttgatg tgcgacaact ccactcgtta tgaattcctt tgagtttctt
     3841 cctgcgacat ccactattac atcatcgaac ttttcattca gagcctgtag tgtttgtgtc
     3901 agattatcaa atttctcaac aagggttatt gctggtaaca gtcctgcagc ttctcgttcc
     3961 gcatgatgtt ttgcggctgt tcgttgtaga tctccgttta acaggcagac ttcccttcct
     4021 ttatttgcga gagcaatagc caggtttgat gcaatggtag ttttccctga accacctttg
     4081 ttcccaccaa ctacagtaat cattgcaacc tcataaatgt gatgtgtgaa gtatgatgat
     4141 attttgacac ggtaacctga ggtgtcaata tgcgttcata tttatgaagt atgaaatatg
     4201 aagtgagtta tgtgatgtaa taaataatac ttgtgttttg tgatttcata actatgatgg
     4261 tgctcaaatt gagtattacc ttgcaaccag aaacagtgat ttactcagat tgagtattta
     4321 aggggctata gatgaacgta tactgcgatg atggttcaac aacaatcaag ttggcatgga
     4381 atgacaatgg gaaaatctgt aagtcgttgt cgcaaaactc atttcgtcat ggatggaagg
     4441 ttgacgggtt agggattcgc cagacattta actatgaact ggatggtaaa aaatacacgt
     4501 atgatgaagt tagtaaccaa tctattctta cgactcatat tgagtatcaa tatacggatg
     4561 ttaacctgtt agctgtgcat cacgctttac tgaatagtgg attagcgcct cagcctgtat
     4621 cattgactgt gactttgcct atcagtgagt tctacacgaa agagtgtcaa aaaaatgaac
     4681 tcaacattca gcgtaaaatt gagaatttaa tgaggccgat tcgtcttaat aaaggcgatg
     4741 ttttcaccat tgaacacgtt gatgtgatgc ctgaatcatt gccagctgtg ttttctcgtc
     4801 tggtcatgga taaagttggt cagttcgaaa aatcgctggt agttgatatt ggcggcacta
     4861 cgcttgatgt tggtgttatc gttggtcaat ttgactctgt aagtgccatt catggtaatt
     4921 ctggcattgg tgtgtcttca gttacgaaag ctgcaatgag tgcattgcgc atggcatcca
     4981 gtgataccag tttcctcgtt gcagatgaat tgattaaacg tcgtaatgac ccggactttg
     5041 ttcggcaggt tataaatgac gaaacaaaaa ctgatctggt ccttaatacc attgaggggg
     5101 ctattgccag tttgggggag caagtagtca atgagctcgg tgatttccat catgtgaatc
     5161 gtgtttatgt tgtcggtggt ggggccccgt tgatctatga ttcgataaaa acggcatggc
     5221 accatcttgg gcaaaaagta gtgatgatgg agtcgccaca gacagcactg gttgaagcta
     5281 tcgcggcttt caaagaggag taatattgtg gacgatgaac ggaaaaggaa aaaatacaca
     5341 ctttatctgc atccggaaaa agctgcagac tttcaaactt tggaagcgat agagtccgtt
     5401 ccgcgttctg agcgtggtga gctgttccgg aatgcattta tttctggaat ggcgttacac
     5461 caacttgatc ctcgcctgcc tgtattgctg actgccattt tgagcgagga gttttcagca
     5521 gatcaggttg taaccttact cagtcagacg actggatgga agccttctca ggccgatatc
     5581 agagctgtgt tgactgaact cggtgcttcg caatctgctg aaaaaatgcc tccttctgct
     5641 acggattcgg tgcagaaggc aatgaatgat gtgcgtctta aaatgaaaaa attattctga
     5701 tatatcagaa tgaagcaatg gggatctcag tccattgtgt tgtatacgaa ggcgatagca
     5761 tttcctgttt catttgccat tcaggggcgg ttcctcgccc cgcaaaccac actttccctt
     5821 ttcccgactg gttcagttca tcgagtgttt tcattaactt ttcactgttt ttacggggct
     5881 ggatttcatc aaataatccc ggctgcgcta tacccgatgg tgtgaaatca gccagcatga
     5941 ctcctgcctt catatagcgg tacccttcac gccagacatg gtttaaggct ctgcatgcgg
     6001 cggcaataat gtcccggctg tcctgtgtgg gcaatgaaag cttttccaca gcggcattgc
     6061 tgtaacaggg ttcttttact gcaaaggggg atgtccgtac aaatgtcgtc acctgccggc
     6121 aatactgacg ctccccacgt agtttctctg cggcccgctc tgcatactga acaacagcct
     6181 ggtgcatggc atctttgtct gtgattcgtt caccaaaact gcgactacag acaatctgtt
     6241 gttttgccgg tggtgcttct tccagggata tgcaggactc gccgttgagt tcgcgtaccg
     6301 tacgctcaag aatgacgctg aagtttttcc ggatgaatgc cgtgttagcc tgcgccagct
     6361 gcagtgctgt gttaataccc agcgcattca gcttttccgt cagtctgcgt cctactcccc
     6421 agacctcacc aactggctgc agccccagta gcttcaagat ccgattacgg ttttctgccg
     6481 tcagcgcgac cacaccggaa aactgtggcc attgctttgt tgcccactgt gcacttttag
     6541 ccagcgtttt tgtaggcgca atgcccaccc ccatggtgag tcctgtccag ctctttacct
     6601 gttccctgag ctgatgacca aaaacctccg gagagatgca atgatttatc ccccgcaaat
     6661 caatgaacat ttcatcaatt gagtagggct caactgcggg agaaagcgac tccagaacag
     6721 ccataacccg ttggctcatg ctgtggtaca gcgcataatt gctggaaaat acatgtattt
     6781 tcttctccag gcgcatttgt ctcacctgaa accagggctg ccccattctg atgccaaggg
     6841 cttttgcctc cgggctgcgc gcgatcacac agccatcgtt attgctgagt acgatgaccg
     6901 gttcgttgcg aaggtccggg cggaaaactt tttcacatga ggcgtagaaa ctgttgatat
     6961 cagccagtgc aaacatcagc gtaactcccg cgtcctgcgt atcacgtgag tgacaacacc
     7021 aaaaatacag atgttttccg gatacagtgt gcggaattcc gggctgtctg aaaccggctc
     7081 cagtgccggg cgtgggcgca acagcagtcg tttgacggtg aactcaccgt cgatctcagc
     7141 gataacgatg tccccgtgtt gtggtttttc gcccctgtct actaccagca gatcaccatt
     7201 ctgcacgcca gcctggttca tcgattcacc gctggcgcgc agaaagaagg tggctgcagg
     7261 tctgctgatg caatagctgt tcagatccag ttcctgctca gcataatcag tggcaggcga
     7321 aggaaaaccg gcctggcaac gatcggcaaa caacggtcgc acgtaactgt catcgcctga
     7381 aggggcagcc ggacggtgat aaacggtact catgctgcag gcagccagct gtcgtcctgc
     7441 cagatttctt ccagcagaga caggatacgt tctttatcct tgtcgttgcg ggctcctgtg
     7501 acagatacag acggggcgct gccctggtct atacgcagcc agatttccgg gtagtgtggt
     7561 ttcagacgtt gttcgatttc attctgtagc gcctgaagtg tgcctgattt gaggtttttt
     7621 gtgctctggc ggtcgaaaag aatttcaatg cggatcatgt tctcatctcc cttttctgtt
     7681 gctgtatgca tatacagtat tctaataact gaatccacac acagtcaaat gtcattattg
     7741 tgactaaaaa acgctcaatt ctctgagggg gtggctgtat gcctgctgtg gactgctgtg
     7801 tccagcttat ccacagcatt ttgcgcacgg ttctgtgaat aaaatacctg gttacccagg
     7861 ccgtgccggc acgttaaccg ggctgcatcc gatgcaagtg tgtcgctgtc gccggcctcc
     7921 tcacccggtc acgtttcgtc gttcctcctc cacgcgctgc ggcttcgggg ccgcacctgc
     7981 attcgtatgc ggtcgcccgg ttacaggtgc ggcacggcct gatggaggcc gcatgtgaga
     8041 ggagaattcc catgccaaac tggtgcagta accgtatgta tttttctgcc gaaccggcac
     8101 agatcgctga gattaaacga ctggccagcg gcgcagtcac accgttttat cgccgcgcca
     8161 caaatgaagg tattcagctg tttctggccg gaagtgccgg acttctgcag accactgaag
     8221 atgtgcagtt tgaaccgtgc cccggactga cggctgccgg acgtggtgtt gtatcgccgg
     8281 agaatatcgc gttcacacgc tggctgacac acctgcagaa cggtgtgcta ctggatgaac
     8341 aaaactgcct gatgctgcat gaactctggc tgcagagcgg tactggacag cgtcgctggg
     8401 aaggattacc ggacgatgtc agggatacca tcaccgcact tttcaccgca aaaagaggtg
     8461 actggtgtgg cttctggagt aacgaggatg tatcggtgtg gtggaaccgt ctgtgtgaca
     8521 acgtactgcc ggaaaaaaac atgccgtttg acctgctgac ggttctgccg acccgcctgg
     8581 atgttgaagt gaatggcttt aacggcggtg ttctgaacgg tgttccttct gcatatcact
     8641 ggtatacaga acggtatggc gtgaagtggc cttgtgggta tgacctgaat atcagcagtc
     8701 agggagacaa cttcattcag gtggatttcg acacgccgtg gtgtcagccg gaaagcgacg
     8761 ttgttgcaga attaagccgc cgtttcagct gcacgctgga gcactggtat gccgaacagg
     8821 gctgtgattt ctgtggctgg cagttgtatg agcgcggaga gctcgttgat gtgctgtggg
     8881 gggaactgga atggtcttcc ccgacagatg acgatgagct gccggaagtc accggacctg
     8941 cgtggatagt cgacaatgtg gcgcattatg gcggatgaag tatgacggag acgggcgggc
     9001 aaccgcccgt ttcttttccg gtaaaggatg tcgccgtgct cctcttttta ctgcgccggc
     9061 tgacgcggcg cggcataaac gcagcctgtg gtcagtgtcc cgcgtccggc gggcacggga
     9121 cgggcgcttt taccgctccc ggctggcgtc ggtgacagtg tacgccagcc tgtcgccctt
     9181 ttctgatgaa cgcccttcaa gccgctttcg cggcataacc ttgccgtcaa acagacgacg
     9241 gctgcggtat tccacggtcg gcctgacccg ttaccggacg cggtgaacag cccacaggca
     9301 gcggggaacg ggcaccacag gggtgccctc ccggtgccca ctgtaagaga aggagttttg
     9361 tatgtcccgt tttgtcctcg gtaactgcat cgatgttatg gcccgtatcc ctgataacgc
     9421 cattgatttc atgtcgggta gcagctgcac attactgtgc agccgccccc taagaaccgt
     9481 acgtgccagt ttcccggcat acggctcaag cctttcaata actccacatt agagccggct
     9541 tcataacaga tattccctga tgttttgcag tgtgatgaca tgccggatgt aacatggcca
     9601 gattacctgt gttatccagt cccccttcgg atttcggcac aatgtgtcga agttgccagc
     9661 ctgttgtggc gttcactggc aggtggcaga cggggcactg acctccctgt cggagccaca
     9721 gatttcttag gcgagagtaa ccctgtatgg attccagcat cgtagctttt agccgtgctt
     9781 caaagtacga ttcgtagctg ggatcaaaag ggttagcagt gctttttatt tgtctgtgtc
     9841 ttttgactgg catatccgag atttcatata gcgccagtaa ctcacgtttg cctgatggca
     9901 ggagcttccc tgtttctgtg ccgaataccc agtgtcggaa gttaactgaa cgaaagtatt
     9961 tctttcttat ccagcctgta cctttctttg ggtgtcgtcg acatgcccag cgccatagtg
    10021 ctttccatat ttcatgactg actttctgaa agatcttttt tgaaacaaca tgacgatagt
    10081 atctggccca gcctttgagg actgggttga gatgccatat cagtgtgctc tggctgactg
    10141 tcggattgtc ttttatcgtc tggcggaccc ttttcaggaa ttcctttacc ttttggggtg
    10201 aaggtttaat caacagtttc ccgttatatt tgcggatgtt catacccaga aagtcgaatc
    10261 catcactgat gtgtgtgatt cgtgtctttt ctggtgagag ggtaagccct ctttctgcca
    10321 gaaagttttc taacaaggga agaacctttt ctgacagcgt gtcgtggctt atccccgtga
    10381 tcacaaaatc gtccgcataa cggattatgt tgatcttttc tggattcagt ccgcctcgat
    10441 agaaggtttt cttcaacaga ggctgcagac catccagaac catgtttgcc agcgttggag
    10501 atattattcc gccctgtgga gttcctgcac tggttgggta taatttcccc aaatcaatat
    10561 atccggcttt cagccatcgt ttcaggatcg ccgtatccgt tgggatattg gcgattagcc
    10621 attcatgtga aatctcatca aaacaactgc gaatgtcggc ttccagaatc cattgtgccc
    10681 gatcagccct gtgcaacgtt gtgaaacatt gctcaatggc atcggctgtt gagcgacagg
    10741 gcctgaaccc aaacgaattt gggtcagccg ttgtttctgc aacaggttcc agcgccatca
    10801 gatagagtgc ctgcatggct ctgtctttca ttgtcgggat ccctaatggt cgctttttgc
    10861 cgttagattt tgggatatat atacgccgta gcggaagagg tttgtaaccc cttcttttca
    10921 gcgtgtatat agccttcatc ttgctgccgg gttttgacca tgttttgcca tcaacacccg
    10981 gcgttctctt ccctctgtta tctgtcactc gtttaactgc cattgctctg gcgctgaacg
    11041 agtgggtgag tagccattgc aaggctttta ccttgcccgc tttctcctcc tgtattgcct
    11101 tcacgatacg cgcctgcagc cttctgacct cgcgacggca cctggcccag ttgatattgt
    11161 gccagtgagt cacgatgtcg gtgggtgcac ccgcgtttat gtttttcgca gtcatctgct
    11221 taccctcctt caaaggtatt caggttatct cgtgttgaaa gaccatgcgg aagtctgccc
    11281 gctttcgcgt ggaatgatgt tgctgaaaca gctcaactcc tgtccatccc attacaggat
    11341 ggcattcgct ttctccgcac tcctgtaccc gcatcactga cagctttcct cgcggttagc
    11401 ctgccctgag ggcagtgata cgggcttacc gtgttccgca cagtttgcag ggatgggtta
    11461 ggttctgcct atccaccggt agctttgatg tccgtgcctc cccagtcaaa agaggaaggt
    11521 ccagctacgt tcccattttg ggtcgggcct gtcagtgcct ttggcccgtt cagggttgac
    11581 gatgtttatc agcagttcac atgtgttaac cctgccatcc agccttgcgc cccatccgcc
    11641 ctgacactgg cagacatgct tcctcctcgc ggattccaca tggtgtgaaa accgggctac
    11701 gttgtcccga aggcttcata ccgaacagtt acccgctctg catgcttcgg taggctacag
    11761 gcgacggaac gcctggtttt actacttcct ttacatgaag ttaaaacaac aaactgtgcg
    11821 actttcacgt cgcaccctca ccgacccgcc gtacctcgtc ggtttccgtg accgttccgg
    11881 gcgcaccatc gctggcgata aaaccgatga atggctgcaa ccggcctgta atgaaatgta
    11941 ccgcgtactc aaaaaagacg cgctgatggt cagcttctac ggctggcacc gtattgaccg
    12001 ctttatggcg gcctggaaac gcgccggatt cagcgtggca gggcacctcg tgttcaccaa
    12061 aaactacaca tccaaatctg catatgtcgc gtaccgccac gaatgcgctt acatcctggc
    12121 aaagggcagt ccggcccggc cccgtaaccc tctgccggat gtgctgggct ggaaatattc
    12181 gggcaaccgt catcacccga cggaaaagcc cgttaccagc ctgcaaccgc tgattgagag
    12241 cttcacacac ccgaacgcga ttgtgctgga cccgtttgca ggcagcggct caacctgcgt
    12301 cgccgccctc cagtccggac gccggtatat cggtatcgag ctgcttgagc agtatcaccg
    12361 tgccggacag caacgtctgg ctgccgtaca gcgggccatg cagcaggggg ccgcaaatga
    12421 tgactggttt atgccggagg ctgcgtaaat gaactatgcc ggacatgaaa aacttcgcgc
    12481 cgaagtggcg gaggtggcca atgccatgtg cgacctgcgt acaaccatga atgagatgga
    12541 gcggcggtac agctttaatg ctgacaccct gccggaacgt ctggtgcgtc agacgttgtt
    12601 tcgcgcaaac cgcctcctga tggaggcata taccgaaatt ctggaactgg attcctgctt
    12661 caaagattga ggggagagag tatgtacgga acatgcgaaa tactctgccg ggagctggca
    12721 gcaaagtatc cgggagacac gccgctgatg ctggttgtct ggtcccctga agagattcag
    12781 gccctcgctg acggaatgga tatttccctg tccgatcatg aaatcagaac cgtcctggcg
    12841 cgcctggagg acatcccgga agaccagcgg actgaattcg gtatttcttc cggcgtggcg
    12901 atggagatca tcaataacgt gagcgaaaac cgccaggtga ccgtccctgc tgaactgctg
    12961 gcgtccctga ttcagaccgc tgaacaggca ttgtggaaac gtgaatgggc cgcccgggat
    13021 catggcctcg ccgtcccgga atgcgtcacc cgccgtcagg cggtgattaa tcaggcccgc
    13081 accctgctga aaaacaacag acacgaaaac gactgatgtt atcgccgcct ccgggcggcg
    13141 attcagggag aacgattaat catgaacgaa aacacaacgc tgaacgcact gatttgtcgt
    13201 cacgcccgca acctgctgct ggcgcagggc tggccggaag agacggatgt tgaccagcgg
    13261 aacccgaagt atccgggctg gatcagcatt tatgttctgc tggatgcgcc ccggctggcg
    13321 acgttactca tcaaccgtca cggcggcgta ctgccgccgc tcctggcctc cgccattcag
    13381 aaactgaccg gaaccggggc ggaactggta ctgtccggca gtcagtggca gtcgctgccg
    13441 gtacttccgg cagacggaac acaggtgttt ttcccgtatg ccggagaatg gctgaccgaa
    13501 gacgaaatca gggcagttct tgatgcggtg cgcgatgcag tacgctgtgt cagttaccag
    13561 gtggcagacg atgcgcggcg tatccgggcg gcgctgacca ccaccggtca gacgttgctg
    13621 acccggcaga cgcgccgctt tcgtctggtt gtgaaggaaa gcgattaccc ctgctggctt
    13681 gatgaagatg acgaaaatct gcccgtggtg ctcgatgcca ttctgaaccg tggcgcccgt
    13741 ttttcttctg tggaaatgta cctggtcagt gagtgtgtgg agcatatcct ggccagtggt
    13801 ctggtctgtg atgtgctgcg tataccggat gaaccgtccc gtcgctggtt tgacagggag
    13861 attctgcggg aggttgttct ggaggcacgg gatgaaatcc gcagcatggc ggatgcactg
    13921 gcgaaaatcc ggaaatgatg acgctggcgg ggaaaaatct ccgccttttc cagccggtcc
    13981 ccggaaagag caaaaggatg tcgccgcagg ctccgcttta cccggccatg cagggcatgg
    14041 ccttgtgggt tttcagttat gtggcctcag cgtcgtgtgc gggctgtgcc gtgcctccat
    14101 cgtagccggg cttctgcggg cgggttgcaa ggtaaggtga accgctgcgg tcacccggtc
    14161 cctccttccc gtctgccgtg attttccggt cgttcacccg ctcagatttc gcggtctcac
    14221 cctccaactc ccgtcagccg tgtgcgtcag gctgcggctt cccttgcgcc cttgcaaccc
    14281 tgcaggcccg gcaaagatgg aggcacggca ccgcccggtg tcgcggaata tgtgaacaac
    14341 ggaggaaatc ccatgcagta tgcgaaacct gtcactctga acgttgaaga gtgcgaccgt
    14401 ctgtcttttc tgccttacct gtttggcaac gattttctgt atgccgaggc gtatgtgtac
    14461 gcgctggcgc aaaaaatgat gccggaatat cagggaggct tctggcattt catccgcctg
    14521 ccggacggtg gcggttacat gatgccggat ggcgaccgtt ttcatctggt caacggtgaa
    14581 aactggtttg accgcactgt gagtgctgat gccgcaggta tcattctgac ttcccttgtg
    14641 attaaccgac agttgtggct gtaccacgac agcggggatg cgggactgac acacctttac
    14701 cggatgcgcg atgcgcagtt gtggagccac atcgaatttc accccgaatg taacgcgatt
    14761 tacgcagcac tggactgatt aacggacggg gcggcgatgc cgcccctgta aaggagacga
    14821 gaatatgtac tgtactgtta aagaaattat ccgcgaagtg ctggatacag acgtgccgga
    14881 cagtgaatgc gtttttgccg tggtgctgac ccgtggggat gtgcgtcaca tagcccagga
    14941 ctggagtctg acagacgatg agctggaaac cgtcatgcag cggctggacg atgcctttga
    15001 gcatggtgct gatgtcagca ttgttcacga cgttgttcgt gaactgatgg aggaaaagcg
    15061 cgtaaaccgt cttgtaacag tccctgcggt gctgctggaa aaagtgatgg tgatggctgg
    15121 tagtgaaatt tatcgcctcc atgctgtagg gagcgagaac gggggcgacg gtgacgcatt
    15181 tgtcagggaa gaacgtgaga ttatgcgggt gatgcgtcag gcgctggacg gggagaatgg
    15241 gtaatgaata tcagcaagga aacccgcgaa attctgcata attacagggc cgtgattaat
    15301 gcgcggcgtc gtgaaatggg gcagaaaccg ctcaccaccg cgcagattgt tgatgaaatc
    15361 tgcgattttg tggcgaatca gcaggcggtt ttcctcggtg gtcactacat ccttcagggc
    15421 agcaggcaca ggtgatacag ggcaggcggt ggaggccgcc tgtcagcccg cgcgaaaacc
    15481 cctggcaggc tggcgcctgc cggaagggat gcgggccacg ctggcgcgtc gcccgttacc
    15541 cttcctgttg gtgacaggcg ttgtttcagg aagtacctgt tacagacctg tcgttaaaga
    15601 accttcttac acatgcgccc cttcggctcc cggctgcctg gcggcatccg gtcgtcaggc
    15661 ccgactccga cgggcagctg ctggcgcacc cgccagtctc cgccgaaccc gctggcgcgg
    15721 tttcgggctg ccgctacggt ccgatgcgtg aaaacccggg cttgcagcga ccgttccggt
    15781 cgcgccgcct ctgtcgcgcc gtgacctgcg ctccctgcac acctgacggt gtgtgaggaa
    15841 actgcgcctg atggcgcagc agtcctccct cctgccgcca cctctgcggc ctgtttcccg
    15901 ctgtggactt tccggggggc agacgtaacc ccctcccgtt ccatctgtcc agggtgagat
    15961 gcaccggcca caaaattttt agccatgaac ccggcaaaaa attttgcgtc cgcccccgga
    16021 cagctgtccc gtgaggcggt aaacgggccc ccatcggaaa ggtccactgc ggggagaaac
    16081 cccagtgaag tggacggcag gaggagcttt cgctcctctg aaggggcaca tgaacgtccc
    16141 gtcgccggta tccggtttat ctcacatctt tcagtcagga ggttcttatg agtgcacgtg
    16201 gtatcaacaa ggtcatcctc gtcgggcgtc tgggcaatga tccggaagtc cgttacatcc
    16261 ccaacggggg cgcagtggca aacctgcagg tggctacgtc agaaagctgg cgcgacaaac
    16321 agacggggga gatgcgggag cagacggaat ggcaccgcgt ggtgctgttc ggcaagctcg
    16381 cggaagtggc aggtgaatat ctgcgcaagg gtgcgcaggt ctacatcgaa ggtcagcttc
    16441 gcacccgtag ctgggaagat aacggtatca cccgttacgt cactgaaatt cttgttaaga
    16501 ccacgggcac catgcagatg ctggggagtg caccacagca gaacgctcag gcgcaaccga
    16561 agcctcagca gaatgggcag ccacagagtg ctgacgcgac gaaaaaaggt ggcgcgaaaa
    16621 cgaaaggccg tggacgtaag gccgcgcagc ccgagcctca gccgcaaccg ccggagggtg
    16681 acgattacgg gttttcagac gatatcccgt tctgactggc ctgactgtga taaccgcccc
    16741 gccctgcgcg gggcatcacc ggagatatga ggatgagcga atatttcaga atacttcagg
    16801 ggctgcctga cggctccttt acccgcgaac aggcggaagc cgttgccgca cagtaccgga
    16861 acgtctttat cgaggatgat cagggaacgc attttcgcct ggttgtccgt caggatggca
    16921 cgttgatctg gcgctcctgg aattttgagg acggtgccgg gtactggatg aaccagtaca
    16981 tcagggattt cgggattctt aagtaagaga ggtgccggac gcgcgaacgt ccggcaggac
    17041 ataagcaatt ataaggggat gattatgcct gtaacgaagt gtgaaccaga aaccacccgc
    17101 aaagcaagcc gtaaatctgt aaaaacgcag gaaactgtcc tgtctgccct gctggcgcag
    17161 acggaggaag tgagcgtgcc gctggcctcg ctgattaaat caccgctgaa tgtgcgcacg
    17221 gtgccgtatt ctgcggagtc cgtcagcgaa ctggcggagt ccattaaggg cgtcggactg
    17281 ctgcagaatc tggtcgtgca taccctgcct ggtgaccgtt acggtgtcgc cgcaggtggt
    17341 cgccgactgg ctgcactcaa catgctggca gagcgtggca tcattccggc tgactggcct
    17401 gtacgcgtga aggttattcc gcaggagctg gcgacggctg catcaatgac tgaaaacggt
    17461 catcgtcggg atatgcaccc tgccgaacag attgccggat tccgtgcgat ggcgcaggaa
    17521 ggcaaaacgc ctgcacaaat cggtgatttg ctgggctatt cgccccgcca cgttcagcga
    17581 atgctgaaac tggcagacct tgcgcctgtc atcctcgatg cgctggcaga agaccgcatc
    17641 accacagagc actgtcaggc gctggcgctg gagaacgaca ccgcgcgtca ggtgcaggtg
    17701 tttgaagccg cctgccagtc gggatggggc ggtaaaccgg aagtacagac cattcgtcgt
    17761 ctggtgaccg aaagtgaagt ggcggtggca ggtaacagta aattccgctt cgtgggggct
    17821 gatgctttct cacctgacga actgcgtact gatttgttca gcgatgacgg ggacggttat
    17881 gtcgaccgcg tggcgctcga tgctgccctg ctggaaaaac tccaggctgt cgctgaacac
    17941 cttcgggaag ccgaaggctg ggaatggtgc gccggacgca tggagcctgt cggtgagtgc
    18001 cgtgaggatg ccggaacata ccgctgtctg ccggagccgg aagcggtgct gacggaggca
    18061 gaagaagaac gcctgaacga actgatgacg cgttacgacg cgctggaaaa ccagtgtgag
    18121 gaatccgacc tgctggaagc agaaatgaag ctgattgact gcatggcgaa ggtcagggcg
    18181 tggacgccgg agatgcgtgc cggaagtggc gtggtggtgt cctggcgtta tggaaacgta
    18241 tgtgtccagc gtggtgtgca gttgcgcagt gaagatgacg cgactgatga cgctgaccgc
    18301 acggaacagg tgcaggagaa agcgtcagtg gaggaaatca gtctgccgtt gctgacgaaa
    18361 atgtcttccg aacgcacgct ggcagtccag gcagcactga tgcagcaacc ggacaaatct
    18421 ctggcactgc tggcatggac gctctgcctg aatgtgtttg acagcggagc gtacagtaaa
    18481 ccagcacaaa tcagcctgga atgtaaacat tattcgctga ccagcgatgc gccgtcaggg
    18541 aaggaaggtg ccgcattcct ggcgctgatg gcagaaaaag cccgtcttgc ggccctgtta
    18601 ccggagggat ggtcacggga catgacgaag ttcctgtcac tcagtcagga ggtgctgtta
    18661 tccctgctca gtttctgcac cgcatgcagc cttaacggtg tccagacccg cgagtgtggt
    18721 cgcacgtcac gcagtccgct tgactcactg gagagcgcca tcggttttca catgcgcgac
    18781 tggtggcagc cgacaaaagc aaacttcttc ggacacctga aaaagccgca gattatcgca
    18841 gccctgaatg atgccggact gtccggtgcc gcacgggacg cagagaagat gaagaaaggc
    18901 gatgccgctg aacatgcaga gttccatatg aaagacaacc gctgggtgcc tggctggatg
    18961 tgttcaccac gtccacagac ggatgccact gaacgcgccg ataacctggc tgatgccgcc
    19021 tgatgaacaa ccacaccgcc ccgccggaga cggggcggca gagggagaaa tacgatgatg
    19081 aataccgaac tgacactgaa tgccctgcag tccatgaatg cccaggaata tgaagaaatc
    19141 cgtgctgcgg gaagcgatat gcgccgtaat ctcactcacg aggtgatgcg taaagtggac
    19201 gcaccggcta actggatgat gaacggagag tatggcagtg agttcggggg ctttttcccc
    19261 gtccaggttc gtttcacgcc agcccacgaa cgtttccacc tggcattatg ttcgccggga
    19321 gacgtctctc agctctggat gctggttctg gtgaatggtg gtggacagcc tttcgccgtc
    19381 gttcaggtgc aacatatctt cacgcctgcc gcgatcagtc acacgctggc gcttgccgcg
    19441 acactggatg cgcagggata cagtgttaac gacatcatcc atatcctgat ggcagaagga
    19501 ggtcaggcat gagcgcacgt tcacgggcac tgatccccct cagcgcagaa caacaggccg
    19561 ccatgcaggc ggtggctgtc acagaacaac gtcgtcgtca gggacgcaca ctttcagcat
    19621 ggccttatgc caccgctttc tttcgctgcc tgaatggcag tcgccggatt tcgctgaccg
    19681 atctccgctt ttttgcccct gcgctgacga aggaggaatt tcatggcaac cgtctcctgt
    19741 ggctggctgc cgtggataaa ctgattgaaa gttttggtga agtctgtgtt cttcccctgc
    19801 catccgatgc ggggcatcgt ctgttcccgt ccgttccttt tcgtgaaggt gagcgtcgtc
    19861 gtcagaaaac cacgctgaca gagcagaaat acagccgcca gcgggaacgt gaggcagaac
    19921 gacgggaact ggaataccag acatgttttg ctcaggcgca gattgacctt gcgtttcata
    19981 ctcccgccac ggtcggaagc tggttgtccc gctggtctgg tgttgttgag gagcatgatc
    20041 tggaaacgat tttctggggg tggtgcgggc gttttccatc actgtcatca tttgaccggt
    20101 ttttctggca ggaggaacca ctctggcggc tgatttttga agccggtgag gccggtcgtg
    20161 gtgcaccggt acaggtacgt gcacttgagc agtggatgat cccgaacaag ctggagaacg
    20221 caatatgatg aaatcagacg aacaatatca ggttcccgtg tggatgcgac ctctgttgcc
    20281 gttgctctgc aataccggtg gtaatgatcc ggaggaactg ctgaatgata cagaaaccac
    20341 cgccagtgcg aatattgtcc gttatgtact gatagttgca gtgcggtcgc agattgatct
    20401 gctgcagcta ctgtacagga aaggactgtt gcgcacagag ataccaggtg gcttttcacc
    20461 ggaagaagcg caggaactcc tggataatct ggtgcgcagc catatcagca aggcgctgtc
    20521 gggtgagcga atggcagccc gtgacagaaa tgccgatctg gcctggattc gccagcaact
    20581 ggtcgatgcc gcctggtttg tccgtgccac actggaagcg catgggatgg gcgtcggaaa
    20641 tgagagtccc tctgctccac cggagacaat gccggacata cagacacggg aactggtgat
    20701 gttgatcaag cgactggcat cttctctgaa agcggtgaaa ccggacagtt gtgtggtgcg
    20761 tgaagcgcag gactggctgc gcgacagaaa acttgtgagc atcacagatg ttcttcggtg
    20821 aaccagtgga ggtatgcttg aggggcaggg acatgccggt catgatcgtt tcatatcagg
    20881 ggtggtctgg aggtcatgcg gcgtgtcctc tgctctcacc ggaataagga agtcgccggc
    20941 ggctccgctt ttacccggcc atgcggggca tggccttgtg ggttttcagt tatgtggcct
    21001 cagcgtcgtg tgcgggctgt gccgtgcctc catcgtagcc gggcttctgc gggcgggatg
    21061 caaggtaaga tgaaccgctg cggtcacccg gtccctcctt cccgtctgcc gtgattttcc
    21121 ggtcgttcac ccgctcagat ttcgcggtct caccctccaa ctcccgtcag ccctgcgcgt
    21181 cgggctgcgg cttcccttgc gcccttgcat cccagcaggc ccggcaaaga tggaggcacg
    21241 gcaccgcccg gtgtcgcaga acatgtgact atggaggatt cgggaatgtc tgttgttgca
    21301 cctgctgtat acgtcggaac ctggcacaaa tacaacggtg gaagcatcgc cggacgctgg
    21361 tttgatctga ccacgtttga tgatgaacgc gattttttcg ccgcctgccg tgctcttcac
    21421 caggatgaag ccgatcctga actgatgttt caggattatg agggattccc ggggaatatg
    21481 gcctctgaat gccatatcaa ctgggcctgg gttgaaggct tccgccaggc acgggatgaa
    21541 ggctgcgaag aggcttatcg tctctgggtg gatgataccg gtgagacgga ttttgacagc
    21601 ttccgcgatg cctggtgggg cgaggctgac agtgaggagg cttttgcggt tgagttcgtc
    21661 agtgataccg gcctgctggc tgacgtgccg gagacggtgg cgctctattt tgactatgag
    21721 gcgtatgcgc gggatttatt cctggactcc ttcaccttta ttgacggtca tgtgttccgt
    21781 cggtgattta ctcccccgct ccggcggggg tatggcctgc cgggccgccg gaaaaccctg
    21841 gcaggcttac gcctgcctga aatacaggag ggccgcgcct gcggcgctgc ccttcctgcc
    21901 tgtcatagtg tgaggcattt ttctgttttg tcgtttctgt tgttctgact cccgggggcg
    21961 gggacaccct tcggctcccg gctgcctggc
//
LOCUS       DAZGVQ010000081        21806 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000081 DAZGVQ010000000
VERSION     DAZGVQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.082"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(286..1206)
                     /gene="malM"
                     /locus_tag="ACHLAG_002971"
     CDS             complement(286..1206)
                     /gene="malM"
                     /locus_tag="ACHLAG_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFP0656157.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(1449..2789)
                     /gene="lamB"
                     /locus_tag="ACHLAG_002972"
     CDS             complement(1449..2789)
                     /gene="lamB"
                     /locus_tag="ACHLAG_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFP0656158.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(2861..3976)
                     /gene="malK"
                     /locus_tag="ACHLAG_002973"
     CDS             complement(2861..3976)
                     /gene="malK"
                     /locus_tag="ACHLAG_002973"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFP0656159.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            4341..5531
                     /gene="malE"
                     /locus_tag="ACHLAG_002974"
     CDS             4341..5531
                     /gene="malE"
                     /locus_tag="ACHLAG_002974"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFP0656160.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            5685..7229
                     /gene="malF"
                     /locus_tag="ACHLAG_002975"
     CDS             5685..7229
                     /gene="malF"
                     /locus_tag="ACHLAG_002975"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFP0656161.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGNGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            7244..8134
                     /gene="malG"
                     /locus_tag="ACHLAG_002976"
     CDS             7244..8134
                     /gene="malG"
                     /locus_tag="ACHLAG_002976"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFP0656162.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            8506..9981
                     /gene="xylE"
                     /locus_tag="ACHLAG_002977"
     CDS             8506..9981
                     /gene="xylE"
                     /locus_tag="ACHLAG_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFP0656163.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(10025..10435)
                     /gene="psiE"
                     /locus_tag="ACHLAG_002978"
     CDS             complement(10025..10435)
                     /gene="psiE"
                     /locus_tag="ACHLAG_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFP0656164.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            10650..10928
                     /locus_tag="ACHLAG_002979"
     CDS             10650..10928
                     /locus_tag="ACHLAG_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656165.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(10975..13071)
                     /locus_tag="ACHLAG_002980"
     CDS             complement(10975..13071)
                     /locus_tag="ACHLAG_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP0656166.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEL
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(13071..13808)
                     /locus_tag="ACHLAG_002981"
     CDS             complement(13071..13808)
                     /locus_tag="ACHLAG_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP0656167.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(13805..14443)
                     /locus_tag="ACHLAG_002982"
     CDS             complement(13805..14443)
                     /locus_tag="ACHLAG_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFP0656168.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVIQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(14557..14799)
                     /gene="yjbE"
                     /locus_tag="ACHLAG_002983"
     CDS             complement(14557..14799)
                     /gene="yjbE"
                     /locus_tag="ACHLAG_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFP0656169.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(15154..16803)
                     /gene="pgi"
                     /locus_tag="ACHLAG_002984"
     CDS             complement(15154..16803)
                     /gene="pgi"
                     /locus_tag="ACHLAG_002984"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFP0656170.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRD"
     regulatory      17040..17236
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            17328..18677
                     /gene="lysC"
                     /locus_tag="ACHLAG_002985"
     CDS             17328..18677
                     /gene="lysC"
                     /locus_tag="ACHLAG_002985"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFP0656171.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVTLTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            18822..19748
                     /locus_tag="ACHLAG_002986"
     CDS             18822..19748
                     /locus_tag="ACHLAG_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFP0656172.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNYIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYSKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLSDKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(19784..20575)
                     /locus_tag="ACHLAG_002987"
     CDS             complement(19784..20575)
                     /locus_tag="ACHLAG_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFP0656173.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVESPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVIYQQDEETITAFKAMVPMWFSPLRARWHYYSMAQKNFPGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAELARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(20581..21708)
                     /locus_tag="ACHLAG_002988"
     CDS             complement(20581..21708)
                     /locus_tag="ACHLAG_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFP0656174.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
BASE COUNT         5197 a         5693 c         5462 g         5454 t
ORIGIN      
        1 cacatcatgt ttcatcaaag agttattgcc gccagaattt gggatatgac ttatcataaa
       61 acctctgcat aacagaaata aaataacatg gaaaagtagc ctgaatgtcc agacactacc
      121 ggcgaaagga aaagaatctc tgataaggca ttgagataat ggatattctt agtgaagaag
      181 aagtgagtta ctataaaaaa tcgctcatat cccaggaagg aagcataaaa aaaggtgcgc
      241 caggagacgc accagttgtt gcaaaatcag cactgtgggg cgtaattacc ccttgccttt
      301 tacactgctg ataaaggtgg aacgggcaga tgtcgatccc aggcgttcag cttcatcaag
      361 cagttttaac gccttatcaa catcaccttt cgcgacagcg tttttgatcg cggtattaaa
      421 ataactttcc gtgtcgttga gcatcggctc gcttttcttc accggtgccg gagcgggtgc
      481 agccacagct ggtgccgccg tgttacctac cgtaaccgga gctggagcgg aagaaccaaa
      541 taaaggtcct accaacacgc tggagctgga gttcgttttc actttcagtt tcagtaagcc
      601 atcggtggta tgacgagcaa ccggatcggg gatatccggg atcgagttac cgacgccctt
      661 ggcataggct ttagccgggt cgagcagttg ggtcgtctgc tggagatctt tttccgtggt
      721 aaagaccaga acataaagtt tttgctgccc caacgccggt gtcaggcgca taacgccttc
      781 cagccgatct gcactcatca cgcctggttc ctggtaggtg aaataactgc tggggaagaa
      841 ggctgatggg gtcatgttct gatcaagaat cagcacgttc ggcgcaaaaa cgctggtttg
      901 tttgttcact tcgctggtca gcgtcagggt cagttcgcca atgtttgccg ggacgctgta
      961 cgcagcaacc ggaccactga tgccgggaac gttcagttgt tggccgccgg tcgccagttg
     1021 ggtggtctgg gttttagatt gatcgaccgg tgtccaggtg agttgttgca gcgcggcaga
     1081 tggaatggct ggcgcgtcgc tggtgttttg cggtacgtag ttaacatcgg caaggctgat
     1141 tccaggcgcg cttgccagta accctgctga taaacagagg gcgatgagac ttttattcat
     1201 tttcattgtt ttcacctcaa aatctggagc tcagcggtag ccaggcaata gcgcgctaaa
     1261 cccgataatc agaggggctt tcgccccttt cagataatga caacctgttt ttatgccgga
     1321 tgcggcgtaa acgccttatc cggcctacat ttgacagccg ttgtaggcct gataagacgc
     1381 gcaagcgtcg catcaggcgt tggttgccga atgcggcgta aacgccttat ccggcccagg
     1441 ttttgctatt accaccagat ttccatctgg gcaccgaagg tccactcgtc gctgtcgcca
     1501 cgaccgaagc tgccgccgtt gaaatcagca ggaacggctt tgccgtagtt cgggttaacc
     1561 ttgctatcgc cgttgtagtc ataaccccat ttctcatccc acttggcgta ggttgcgaag
     1621 acacgaatag ccgggcgtga ccagatgctg tcgccagcct gccattgttg tgcgagggta
     1681 attttgtact gattgttctt gtcgccggtg cgctgggatt cgacgttgtc gtagccgatt
     1741 tccatcacgg tgctcatgat tggcgtccac ttgtacatcg ggcgaatacc aacggtccac
     1801 cacttggtgc cgttgtcgtt atcccagttg atatcctggt acatacccac gtacatcatg
     1861 tcccagttgt cgcccatgga gatcgcaccg tggtcgagga tacgcagcat gtgaccgttg
     1921 ttgttgatat tgtaggcaaa tttttcgtta tcaaacgcga cgccagaacc ctgcgacaga
     1981 cctttaccct gcgaggtcat cgagtcagta gcgtactgaa caacaaactt gttaaagccc
     2041 ttcaggacac tctgagtatg ttcagcagtg aataaccagc cgtctttcga tgcgccatca
     2101 accagacgat agttatcacg caggttggca cgaccgtagt cgacacccag ttctaatgtg
     2161 ccgcccgggt tgatttccat ctgcgctaaa cgcacatcga aaacgtcgtt cgcggtttcg
     2221 ttggtatagt cataaatatt gttgctggca aaagaggaag aaccaccagc ttcagaggag
     2281 cgggttgctg ccagagagag tttaccgaag ccaacatcga tgttttccag accggcacca
     2341 ggaccagaaa tatcccagta gtagaagtcg atcatatgaa cgtcatgacg ttggtagaag
     2401 cgcttacctg cccagatggt ggaacctggc agccattcga tcaggttttt accctgcacg
     2461 tttgcttcac ggaaggccgg atcggtagct tcccagtcat tctgttgcgc gacggaatag
     2521 gccacgttag tgtcgaaata gaagctctta tcgccctctt tccacacttc ctgacccaat
     2581 tttaattcag cataagtttc acattcgttg ccaagacggt atttactttg agcaccggta
     2641 gtctggaaac actgttgttc accgccgcta cctgtccagc caataccgga acgtgcatag
     2701 ccgtggaaat caacagccat tgcctgagca gacattacgc ccgctgcgac ggcaaccgcc
     2761 agaggaagtt tgcgcagagt aatcatcatt ctatctcctg agtcattgct tttctttttt
     2821 cacatcacct gtgacaggct ttgtgtgttt tgtggggtgc ttaaacgccc ggctccttat
     2881 gcagtcgacg acatgcagtg ccatcctcac ggaacagatg gcaacgctct ggcggcaggc
     2941 cgatagcgaa tgtggcacct tcttctacca acaccacgtc gttctggcgg tacaccaggt
     3001 tttgacgaat ggaagggatc tggatatgga tttgggtttc gttgccgagt tgctcgacga
     3061 cctgaacttc accctcaagg atgacgtcag cgatatcact cggcagtaga tgttccgggc
     3121 gaatacccag cgacatattg gctccaacct ggacatcacg gctttcaact ggcagccaga
     3181 cttgctgacg atttggcatc ggcagctcca cctgcacttg atcgattgcg gtggcggtca
     3241 cttttaccgg caggaagttc atctttggcg aaccgataaa tccggcgaca aaacggtctg
     3301 ccggatagtg gtacagctcc agcggtttcc caacctgcgc cacgcgaccg gcgtccagca
     3361 ccacgatttt gtcggccagc gtcatcgctt cgacctgatc gtgggtgacg taaatcattg
     3421 tgcggcccag gcgtttatgc agacgggaga tttcgatacg catttgcaca cgcagtgcag
     3481 catcgaggtt ggagagcggt tcatcgagca aaaatacgct tggctcggcc accagcgtac
     3541 ggccaatcgc cacacgctga cgctgaccgc cagagagcgc tttcggtttg cgatccagca
     3601 aatgcgccag ttgcagcact tccgccacct ggttaacgcg ttggttaatc acctcttttt
     3661 ttgcgcctgc cagtttcagg ccaaatgaca tattttctgc tactgacagg tggggataga
     3721 gcgcgtaaga ctgaaacacc ataccaacgc cgcgttctgc tggcggagtg tcattcatcc
     3781 gtttctcacc gatgaacagg tcgccgctgg tgatcgtctc aagcccggca atcatgcgca
     3841 gtaaagtcga tttaccgcag ccagacggtc cgacaaacac cacgaattca ccttcatgga
     3901 tatcgagatt gatatctttc gataccacga cctcgcccca ggctttcgtt acattttgca
     3961 gctgtacgct cgccatgccc ttctcccttt gtaacaacct gtcatcgaca gcaacattca
     4021 tgatgggctg actatgcgtc atcaggagat ggcttaaatc ctccaccccc tggctttttt
     4081 atgggggagg aggcgggagg atgagaacac ggcttctgtg aactaaaccg aggtcatgta
     4141 aggaatttcg tgatgttgct tgcaaaaatc gtggcgattt tatgtgcgca tctccacatt
     4201 accgccaatt ctgtaacaga gatcacacaa agcaacggtg gggcgtaggg gcaaggagga
     4261 tggaaagagg ttgccgtata aagaaactag agtccgttta ggtgttttca cgagcacttc
     4321 accaacaagg accatagatt atgaaaataa aaacaggtgc acgcatcctc gcattatccg
     4381 cattaacgac gatgatgttt tccgcctcgg ctctcgccaa aatcgaagaa ggtaaactgg
     4441 taatctggat taacggcgat aaaggctata acggtctcgc tgaagtcggt aagaaattcg
     4501 agaaagatac cggaattaaa gtcaccgttg agcatccgga taaactggaa gagaaattcc
     4561 ctcaggttgc ggcaactggc gatggccctg acattatctt ctgggcacac gaccgctttg
     4621 gtggctacgc tcaatctggc ctgttggctg aaatcacccc ggacaaagcg ttccaggaca
     4681 agctgtatcc gtttacctgg gatgccgtac gttacaacgg caagctgatt gcttacccga
     4741 tcgctgttga agcgttatcg ctgatttata acaaagatct gctgccgaac ccaccaaaaa
     4801 cctgggaaga gatcccggcg ctggataaag aactgaaagc gaaaggtaag agcgcgctga
     4861 tgttcaacct gcaagaaccg tacttcacct ggccgctgat tgctgctgac gggggttatg
     4921 cgttcaagta tgaaaacggc aagtacgata ttaaagacgt gggcgtggat aacgctggcg
     4981 cgaaagcggg tctgaccttc ctggttgacc ttattaaaaa caaacacatg aatgcagaca
     5041 ccgattactc catcgcagaa gctgccttta ataaaggcga aacagcgatg accatcaacg
     5101 gcccgtgggc atggtccaac atcgacacca gcaaagtgaa ttatggtgta acggtactgc
     5161 cgaccttcaa gggtcaacca tctaaaccgt tcgttggcgt gctgagcgcc ggtattaacg
     5221 ccgccagtcc gaacaaagag ctggcgaaag agttcctcga aaactatctg ctgactgacg
     5281 aaggtctgga agcggtcaat aaagacaaac cgctgggtgc agtggcgctg aagtcttacg
     5341 aggaagagtt ggcgaaagat ccacgtattg ccgccaccat ggaaaacgcc cagaaaggtg
     5401 aaatcatgcc gaacatcccg cagatgtccg ctttctggta tgccgtgcgt actgcggtga
     5461 tcaacgccgc cagcggtcgt cagactgtcg atgaagccct gaaagacgcg cagactcgta
     5521 tcaccaagta atgctatgaa atgccggatg cggcgtgaac gccttgtccg gcctacaaaa
     5581 ctgaaacgca tgtaggcctg ataagacgca tcagtgtcgc atcaggcagt tgttgtcgga
     5641 taaggcgtga aagccttatc cgtcctggaa tgaggaagaa ccccatggat gtcattaaaa
     5701 agaaacattg gtggcaaagc gacgcgctga aatggtcagt gctaggtctg ctcggcctgc
     5761 tggtgggtta ccttgttgtt ttaatgtacg cacaagggga atacctgttc gccattacca
     5821 cgctgatatt gagttcagcg gggctgtata ttttcgccaa tcgtaaagcc tacgcctggc
     5881 gctatgttta cccgggaatg gctggaatgg gattattcgt cctcttccct ctggtctgca
     5941 ccatcgccat tgccttcacc aactacagca gcactaacca gctgactttt gaacgtgcgc
     6001 aggaagtgtt gttagatcgc tcctggcaag caggcaaaac ctataacttt ggtctttacc
     6061 cggcgggcga tgagtggcaa ctggcgctca gcgacggcga aaccggtaaa aattacctct
     6121 ccgacgcgtt taaatttggc ggcgagcaaa aactgcaact gaaagaaacg accgcccagc
     6181 ccgaaggcga acgcgcgaat ctgcgcgtga ttacccagaa ccgccaggcg ctgagtgaca
     6241 ttaccgccat tctgccggat ggcaacaaag tgatgatgag ctccctgcgc cagttttctg
     6301 gcacgcagcc gctctacaca ctcgacggta acggcacgtt gacgaataat cagagtggcg
     6361 tgaaatatcg tccgaataac caaattggct tttaccagtc cattaccgcc gacggcaact
     6421 ggggggatga aaagctaagc cccggttaca ccgtgaccac cggctggaaa aactttaccc
     6481 gcgtctttac cgacgaaggc attcagaaac cgttcctcgc cattttcgtc tggaccgtgg
     6541 tgttttcact gatcaccgtc tttttaacgg tggcggtcgg catggttctg gcgtgtctgg
     6601 tgcagtggga agcgttgcgt ggcaaagcgg tatatcgcgt cctgctgatt ctgccctacg
     6661 cggtgccatc attcatttca atcttgattt tcaaagggtt gtttaaccag agcttcggtg
     6721 aaatcaacat gatgttgagc gcgctgtttg gcgtgaagcc cgcctggttc agcgatccga
     6781 ccactgcccg aacgatgctg attatcgtca atacctggct gggttatccg tacatgatga
     6841 tcctctgcat gggcttgctg aaagcgattc cggacgattt gtatgaagcc tcagcaatgg
     6901 atggcgcagg tccgttccag aacttcttta agattacgct gccgctgctg attaaaccgc
     6961 tgacgccgct gatgatcgcc agcttcgcct ttaactttaa caacttcgtg ctgattcagc
     7021 tgttaaccaa cggcggcccg gatcgtcttg gcacgaccac gccagccggt tataccgacc
     7081 tgcttgttaa ctacacctac cgtatcgctt ttgaaggcgg cgggggtcag gacttcggtc
     7141 tggctgcagc aattgccacg ctgatcttcc tgctggtggg tgcgctggcg atagtgaacc
     7201 tgaaagccac gcgaatgaag tttgattaag ggagataaca aaaatggcaa tggtccaacc
     7261 gaaatcgcaa aaagcacgtt tgtttattac tcacctgcta ctgctacttt ttatcgcggc
     7321 gattatgttc ccgctgctga tggtcgtcgc tatctcgctg cgtcagggaa acttcgcgac
     7381 cggtagcctg atcccggagc aaatctcctg ggatcactgg aaactggcgt taggttttag
     7441 cgttgaacag gctgatggtc gcattacgcc accgccattc ccggtactgc tgtggctgtg
     7501 gaactcggta aaggtcgccg ggatttccgc gattggcatt gtggcgctct ccaccacctg
     7561 cgcctacgct ttcgcccgta tgcgctttcc aggcaaagcg acgctgctga aaggaatgct
     7621 gattttccag atgttcccgg cagtactttc actggtcgcg ttgtatgcgt tgtttgatcg
     7681 tctgggtgag tacattccat tcattggcct gaatactcac ggcggcgtaa ttttcgcgta
     7741 tctgggtggg attgcgctgc atgtctggac catcaaaggc tatttcgaaa ccatcgacag
     7801 ttcgctggaa gaagcggctg cgctggatgg tgcgacaccg tggcaggcct tccgccttgt
     7861 cctgttgccg ctgtcagtac cgattctggc ggtggtattc atcctgtcgt ttatcgctgc
     7921 cattactgaa gttccggtcg cgtcgctgtt actgcgtgac gtaaacagct acaccctggc
     7981 cgtggggatg cagcaatacc tcaacccgca aaactacctg tggggtgact ttgccgccgc
     8041 tgccgtgatg tctgcattac cgatcaccat cgtcttcttg ctggctcaac gctggctggt
     8101 caacggcctg acggcaggtg gtgtgaaagg ttaaagatgt tgttctgcca atgttatgcc
     8161 gctgcaccct caacttacgt tatcccaact tgtgactgtt attcggcgct ccacggagcg
     8221 cctttttttc tttcgtctgc aatctgaatc gttcgccggt taatattccc atcatagagc
     8281 ttattatttt tacgttattt gttttcccac ttacgataat tctctttcgt gctctgagtc
     8341 acggcaatag tattgttttc atcaattttt gataattatc acaattaaga tcacagaaaa
     8401 gacattacgt aaacgcattg taaaaaatga taattgcctt aactgcctga caattccaac
     8461 atcaatgcac tgataaaaga tcagaatggt ctaaggcagg tctgaatgaa tacccagtat
     8521 aattccagtt atatattttc gattacctta gtcgctacat taggtggttt attatttggc
     8581 tacgacaccg ccgttatttc cggtactgtt gagtcactca ataccgtctt tgttgctcca
     8641 caaaacttaa gtgaatccgc tgccaactcc ctgttaggat tttgcgtagc cagcgctctg
     8701 attggttgca tcatcggcgg tgccctcggt ggttattgca gtaaccgctt cggccgtcgt
     8761 gattcactta agattgctgc tgtcctgttt tttatttctg gtgtaggttc tgcctggcca
     8821 gaacttggtt ttacctctat aaacccggac aacacagtcc ctatttatct ggcaggttat
     8881 gtcccggaat ttgttattta tcgtattatt ggcggtattg gcgttggttt agcctcaatg
     8941 ctttcaccaa tgtatattgc ggaactggct ccagctcata ttcgcggaaa actggtttca
     9001 tttaaccagt ttgcgattat tttcgggcaa cttttagttt actgcgtaaa ctattttatt
     9061 gcccgttccg gtgatgccag ctggctgaat actgacggct ggcgttatat gtttgcctcg
     9121 gaatgtatcc ctgcactgct gttcttaatg ctgctgtata ccgtgccaga aagtcctcgc
     9181 tggctgatgt cacgcggcaa gcaagaacag gcggaaagta tcctgcgcaa aattatgggc
     9241 aacacgcttg caactcaggc agtacaggaa attaaacact ccctggatca tggccgcaaa
     9301 accggtggtc gtctgctgat gtttggcgtg ggcgtgattg taatcggcgt tatgctctcc
     9361 atcttccagc aatttgtcgg catcaatgtg gtgttgtact acgcgccgga agtgttcaaa
     9421 acgctggggg ccagcacgga tatcgcgctg ttgcagacca ttattgtcgg agttatcaac
     9481 ctcaccttca ccgttctggc aattatgacg gtggataaat ttggtcgtaa gccactgcaa
     9541 attatcggcg cgctcggaat ggcaatcggt atgtttagcc tcggtaccgc gttttacact
     9601 caggcaccgg gtattgtggc gctactgtcg atgctgttct atgttgccgc ctttgccatg
     9661 tcctggggtc cggtatgctg ggttctgctg tcggaaatct tcccgaatgc cattcgtggt
     9721 aaagcgctgg caatcgcggt ggcggcccag tggctggcga actacttcgt ctcctggacc
     9781 ttcccgatga tggacaaaaa ctcctggctg gtggcccatt tccacaacgg tttctcctac
     9841 tggatttacg ggtgtatggg cgttctggca gcactattta tgtggaaatt tgtcccggaa
     9901 accaaaggta aaacccttga ggagctggaa gccctctggg aaccggaaac gaagaaaaca
     9961 caacaaactg ctacgctgta atcttcctgt ccagcacgcc gcgccagttc ggcgtgctgt
    10021 ctttttactc ccgcttcagc cgtttcgaat tacacaacca cagagtgatc accagtaaca
    10081 ggatcgcagc cgagtagatc aacacatcca gtggcgattt atgatcgacg atgatcaagc
    10141 gcacaatggc ggtgatccca atatagacaa agtaacgtag cgggaagtga aaaccggact
    10201 gaaagtattt cacaatcagc gcgataaatt cgaaatagag aaagtaaacc accagtcctt
    10261 ctaccagctc atatttgctg gtttgttctg gcgcgaacag cacatctgcc agatgcaccg
    10321 tttctttgcc gaggaagaca accaaaatca ggccgaggca caacaggcca agattgagta
    10381 cggtctgcaa aatggtggag ataaactcca cgcgcggacg agagagtgac gtcatagcag
    10441 tacctccttc acagtggcga gcttcctgta taacgcaaaa atgtgacgga gatctatatt
    10501 ttttgtgtat gttttgttca gcttgattaa gtgggcatct cccatcaacg tcgcatctgg
    10561 caaaacacac agccccaaaa aatcacccat tcaggtgatt cccagccacc agcaatgcgc
    10621 gaaaaatcga ccatgttcct ctggaacaaa tgaaacgtaa cctgataaag gtggtaaaaa
    10681 tgaagcctta ttttgctgct ttgatgttat cagtctctgt tcttcctgct tatgctggtc
    10741 cgctcggtac tgctgataaa gccgacctac cgcaaagcaa tgtctccagc ccaatgatgg
    10801 cccagtcgct caggcaacca gatctgcaac cgatatcgac ggacaggaag acagaatgtt
    10861 ttcgccttta cacaccagat cgcaaaccgg gggtgaactg cgttcctgat ggctcaacag
    10921 gccattaaga atgagggtgc cagtgattgc gaaaactggc acctttccca tgacttagcg
    10981 gaaattgacg ctacggtcgc tggtcatgtc atacaactgg aacttacgcc caagttgctg
    11041 accaccgtca cgcgtcagcg gcgtccagcc aatcgccgca cggctgcgtg tcgggccaga
    11101 cgagaagaga tccaacggta ccgagacata cacgcctttg gtgaagtccc cttcgccgta
    11161 ctcctctttt gaaacattag tgatcgtggc atagccaccc accaccacgc cgctatcaaa
    11221 gcgtttggcg atctccagcg tgccgccttt atcccctgcc agatactgcc cgacgctggc
    11281 tttaactaac acatcctgag cgaaagatgg cgtccagtag gcggtcagat gtccggtttt
    11341 cacgctgtag tcggtgaatt tcatcatatc ttttgcacta cgccagtcgc gctgtttaac
    11401 gtagttggca tccagaccaa acgcccagtt gctgtccagc gggcgataca acacttctgc
    11461 ccccgcaccg ccaaacatgg tttcgagata accaccgtag acctgaccgt agaagccgtt
    11521 gcccagatgc tggaagtagt tggcttgcag gttattcaca tagacatcgt tctgcacata
    11581 ctcgcgcaca tgggtacgca cgcgcggtaa gtgcgagtcc tgcggaggat tagtgtagtt
    11641 aaacttgtcg tagttgttgg caagatttgc aaacaggctg ccggtcgtca gcagatggtc
    11701 cgtcagccac aaatctgccg ttcccatcac gcccagctga tacatgtaaa agttttccgg
    11761 gccaccgacc gactggttca gcaccggatc gatatggaaa tcaaagcgtg atttgtcgat
    11821 ataccatccc tgctcggtgg actgcggaac cactggctcg acgcgttttt gcgccagcgt
    11881 cgtttcgtgg cccaacagct ctccggcgag atgatttttc aggctggcga catcggtttc
    11941 cgtcgtcacc tgcggcatgt taaggcgatt ttccgtaacg cggatcgttt tgatcccatc
    12001 cggcagatca ttcatcacga tccgattagc acggatgatc ccttcgcgcg aatcacgata
    12061 tttcacctgc tcgccggtaa catacagcgt atcgcctttc gcctggatct gcggatcggc
    12121 aagtccggca ttgtatttca acagcgttaa ctgattcgcc accaccgaat gctgcaaaat
    12181 ggcatcctgc ggctgcggtt gatattgcgg gcgggcgtta tcgttgtaag acgggcgcag
    12241 atcgttaaag ttggtgcgca gcgtgacgcc aaacataaag gtgttgccac gttcatagct
    12301 aaggttaacg tcggcccaat cggtaacgcg ataaatcgca ccgacgttaa acttactttt
    12361 ttgctccagc ttcccggcaa aatcctgctg ataattattg ccttcatact ccagtttcag
    12421 gcgcagcggt tgccagggcg tctggtattc cacgccgcca aacagtgagg caggaccgtg
    12481 gaacatctgg ctaccgtcga tagatcccgc ctgtttgtag ctgttatcgc gatagcaata
    12541 tttatcactg gctgaacaga gcggattttt tacattaccg ctggtgccca gatatcccca
    12601 gcccaggccg agcgtaaaat cgaacggccc ccaggctttg ctggcaacaa gatattccgc
    12661 atcaaacagc cccgtaccgc cgatatcccg cgcaccaacc gccacttgcg gcagccagta
    12721 actctcttcc cacaaacgca gtttgagatc gaaggcttta tctttatacg tttgatcgcc
    12781 agagaacgct tcgacgctgc tgtactgccg ggtgcgcacg tcggtgtagc gcagcgttgt
    12841 ttccagccac gggaagagtt gcactgaagc tgagtaataa cggtactgat cgttatcgcg
    12901 atagttcaga ctcaactccc cttcccgtgc catacgcgcg gtgggagttt gtaataatcc
    12961 tacgccaccg aaatccgact gcgacggacc aatgggcgca ggatatgttt cgccgtagca
    13021 agctgtgcta atgcccagcg ccagtaagct aagcagatgt ctttttttca ttattggggt
    13081 atccgctgcg tcagagtctg aagaatgtcg gcgttaaggg catcaggcgt ctcactccag
    13141 acggagtccg cgaggccaac ataaataatg ctgccgggca tcggctctac gtgacgcttg
    13201 ttccagtaag ccaccggcgc tttttgcgtg cgtccgtccg ggtaaaccac ccacgcgtag
    13261 ctgcgatccg caccgctaag caggttctgc ccggagagat agctcgccac gtcgcgacct
    13321 ggagtgaatg gctgcttgcc aggacggctg ataagcccga acagcgtgac cgtggacggt
    13381 ggtggcccga cccacagcgt gtagttgccc tgcaacggtg ggttaccgcg ttcggcaacg
    13441 cggacgatat cgggatcaag attgattttt tgcctgcccg tcactttcaa cgcctgaatt
    13501 tgctggcgta aggcgttaat cgcggcggca tcgtcggcgc tggaatctgc agccagttct
    13561 gccagccgtg tcagcaacgc ctgctgctgc cgcaatgctg ccgccgttgc cagctcttcg
    13621 ctaatcaccg caccaggcca ccaactgttt gccagccgcg gctggcctac cagatcgatt
    13681 aaatactctg cgcccgtcag cgttttggcc tcactgctgc cattgctgaa taccttgacg
    13741 gttcccgccg cgaagaccga tgacgccccc acactcaaaa tcaatgcgac aatagtttgt
    13801 ttaatcattg ttttgccgcc ttgatgagag tggttttcac cgggaaataa tccgcgccga
    13861 gatactgttc cgactggcga atttgccctt cgctgtcgat ccagtagcgg ttatgccagc
    13921 gcgcctggtc ggtggagact tcttcgtcca gcacgcgaac cggggtttca tcgctgccga
    13981 ccttgacggt atcggtgcca tcccatttga agactgagcg tgcggtggcg tagcgtacct
    14041 gctggtactc ggtccagccc atcgtgcgcg tccatgttgc accatcaaca atttgtgcgg
    14101 gtttaatcag cgggtcggcg gcaaggttat tcacttcaat caggttgtcg ccgccaagca
    14161 aagtcttcac cagacgacca tgttgtatga caagagtggc ttgatcctga gtgacccatt
    14221 tttgttgtcc gtcttcagcg aaggccagca ccacaaacaa ttgcggcccg ccattaagct
    14281 gcatgtactg gctggcgtag ggcatatttt gaatatcatc gtccgtcagc tgtacgcctg
    14341 gcgtgccgaa cagactgtcc cacagtgaat tgcccagctc tttagtcgtg gctgaacagg
    14401 cctgtaatag caggcagata agaatgagtg caggtcgctt cacgactctt ctccagaggg
    14461 gcagaataac cacaccggag tgtggttatg gtaaatccct tcatacttca agccacagat
    14521 gcaatgactc aaattatttt ggggatacat actttattac tgggtactgg tggtcgtggt
    14581 tgtggtggtc ccggtattag aaccatcacc gccaccggtt gccgcgagcg cgacacccac
    14641 cgccgagctt acggtgctga cgctggtcgc ggaggaactc cccgccgaaa ccgacgttgc
    14701 tgccgaccct gccgcttcgc ccacctgtac aggtgcagcc cacgcagaag tcgccgcaag
    14761 cgcagatatg gcaaaaatgc catacagaac ttttttcata acaatttccc ttcattgaat
    14821 gaatggagat ttacccaaaa acatttcggg cgggatcgag tatacacaac taaagcatgc
    14881 gaattacggc gcggggaatt agagatgggg ttttaggata attggaatga atatcaaata
    14941 taagctacaa cttatatata aataacatta aaaatcataa tgttaaaata aaagtaaaag
    15001 taattaatct gagtattttc cgaaatcaat cacgattaaa aatctatttt aggattaacc
    15061 tcacggtatg atttccgtta aatcacagac aaggggattt atctgataaa acaatgccga
    15121 cataacgtcg gcattgttat taaggttttt aaattaatcg cgccacgctt tatagcggtt
    15181 aatcagacca ttggtcgagc tatcgtggct gctgatttct ttatcatctt tcagctctgg
    15241 cagaatacgg tttgccagtt gtttacccag ctccacgccc cactggtcga aggtgaagat
    15301 gttcaggatc acgccctgag taaagatttt gtgctcatac agcgcaatca acgcccccag
    15361 gctgaacgga gtgatttcac gcagcaggat ggagttggtc gggcggttac cttcgaacac
    15421 tttgaacggc accacgtagt caagcgttgc cggatcttta ccctgatcgc gatattcctg
    15481 ctcaaccact tcgcgggatt taccaaacgc cagcgcttca gtctgagcaa agaagttaga
    15541 cagcagtttc tggtggtgat cagagagcgg gttatgggtg atagccggag cgatgaaatc
    15601 gcacggtacc attttggttc cctggtggat cagctggtag aacgcgtgct gaccgttagt
    15661 gcctggttca ccccagataa tcgggccagt ctggtaatcc acaacgttac cgttacggtc
    15721 aacatactta ccgttggact ccatattgcc ctgctggaag tacgccgcga aacggtgcat
    15781 atactggtca tacggcagaa tcgcctcagt ttccgcacca aagaaattgt tgtaccagat
    15841 gccaatcagc gccagcagta caggcaggtt tttctcggca ggcgtggtgg agaaatgctt
    15901 gtccatcgcg tgtgcgccag aaagcagttc aacgaagtta tcaaagccga tggagagaac
    15961 aatcgacagg ccaatcgctg accacaaaga gtaacggccg ccgacccagt cccagaactc
    16021 gaacatgttg gcagtatcaa taccaaactc gccaacggct ttggcattgg ttgagagcgc
    16081 cgcaaagtgt ttcgcaacgt gtttttcatc acctgccgct ttcaggaacc agtcacgcgc
    16141 gctatgggcg ttggtcatgg tttcctgagt ggtgaaggtt ttagatgcta ccaggaacag
    16201 cgtggtttcc gggtttactt ttttcagcac ttccgcgatg tgagtcccat cgacgttaga
    16261 aacaaagtgc atgttcaggt ggtttttgta cggacgcagt gcttcggtca ccatgtacgg
    16321 gccgaggtca gaaccgccga tcccgatgtt cactacgtca gtgattgctt tgccggtata
    16381 acccttccac tcaccggaga taatcgcttc cgagaaggtt ttcatcttct ccagcaccgc
    16441 gttgacttcc ggcattacgt ctttgccatc aaccaaaatc ggggtattgc tacggttacg
    16501 cagcgctacg tgcagcacag cgcggttttc agtgcggttg atcttctcgc cagagaacat
    16561 cgacttaatc gcgcccgcca gatcgcactc tttcgccaga tcttgtaatt tcgccagcgt
    16621 ctcttcagtg atgcggtttt tggagtaatc caccagcatc tgatcgccga aggttgcgga
    16681 gaacttagaa aaacgatcgc cgtctttagc aaaaagatcg gcgatcgtaa cgtctttcat
    16741 ttcatcgaag tgtttctgta gtgcctgcca ggcagcggtc tgcgttggat tgatgttttt
    16801 cattagcaat actcttctga ttttgagaat tgtgactttg gaagattgta gcgccagtca
    16861 cagaaaaatg tgatggtttt agtgccgtta gcgtaatgtt gagtgtaaac ccttagcgca
    16921 gtgaagcatt tattagctga actactgacc gccaggagtg gatgaaaaat ccgcatgacc
    16981 ccgtcgttga caaccgcccc gctcaccctt tatttataaa tgtactacct gcgctagcgc
    17041 aggccagaag aggcgcgttg cccaagtaac ggtgttggag gagccagtcc tgtgataaca
    17101 cctgaggggg tgcatcgccg aggtgattga acggctggcc acgttcatca tcggctacag
    17161 gggctgaatc ccctgggttg tcaccagaag cgttcgcagt cgggcgtttc gcaagtggtg
    17221 gagcacttct gggtgaaaat agtagcgaag tatcgctctg cgcccacccg tcttccgctc
    17281 ttcccttgtg ccaaggctga aaatggatcc cctgacacga ggtagttatg tctgaaattg
    17341 ttgtctccaa atttggcggt accagcgtag ctgattttga cgccatgaac cgcagcgctg
    17401 atattgtgct ttctgatgcc aacgtgcgtt tagtagtcct ctcggcttct gctggtatca
    17461 ctaatctgct ggtcgcttta gcggaagggc tggaacctgg cgagcgattc gaaaaactcg
    17521 acgctattcg caacatccag tttgccattc tggaacgtct gcgttacccg aacgttatcc
    17581 gtgaagagat tgaacgtctg ctggagaaca ttactgttct ggcagaagcg gcggcgctgg
    17641 caacgtctcc ggcgctgaca gatgagctgg tcagccacgg cgagctgatg tcgaccctgc
    17701 tgtttgtcga aatcctgcgc gaacgcgatg ttcaggcaca gtggtttgat gtacgtaaag
    17761 tgatgcgtac caacgaccga tttggtcgtg cagagccaga tgtagccgcg ctggcggaac
    17821 tggccgcgct gcagctgctc ccacgcctca atgacggctt agtgatcacc cagggattta
    17881 tcggtagcga aaataaaggt cgtacaacga cgcttggccg tggaggcagc gattatacgg
    17941 cagccttgct ggcggaggct ttacacgcat ctcgtgttga tatctggacc gacgtcccgg
    18001 gcatctacac caccgatcca cgcgtagttt ccgcagcaaa acgcattgat gaaatcgcgt
    18061 ttgccgaagc ggcagagatg gcaacttttg gtgcaaaagt actgcatccg gcaacgttgc
    18121 tacccgcagt acgcagcgat atcccggtct ttgtcggctc cagcaaagac ccacgcgcag
    18181 gtggtacgct ggtgtgcaat aaaactgaaa atccgccgct gttccgcgcg ctggcgcttc
    18241 gtcgcaatca gactctgctc actttgcaca gcctgaatat gctgcattct cgcggcttcc
    18301 tcgcggaagt tttcggcatc ctcgcgcggc ataatatttc ggtagactta atcaccacgt
    18361 cagaagtgag cgtgacatta acccttgata ccaccggttc aacctccact ggcgatacgt
    18421 tgctgacgca atctctgctg atggagcttt ccgcactgtg ccgggtggaa gtagaagaag
    18481 gtctggcgct ggtcgcgttg attggcaatg acctgtcaaa agcctgcggc gttggcaaag
    18541 aggtattcgg cgtactggaa ccgttcaaca ttcgcatgat ttgttacggc gcatccagcc
    18601 ataacctgtg cttcctggtg cccggcgaag atgccgagca ggtggtgcag aagctgcatt
    18661 ttaatttgtt tgagtaatat ttccctactg gcgtataacg ctcaccgatt gttatacgcc
    18721 atcctcactg aaaacataga ttaaataata tctgaaaagc aaaatcactc ctgtagaaca
    18781 gtattataat caccgaaaaa tatcatttaa caggagtgtt aatggctact ttaatggaaa
    18841 aagatgcact tttaaacgga gccagtcagt gcatcgcgtt tttatcaaat atcattgata
    18901 attgctcagt ttcgtcgcat caggattcag gcgatgctct caagcgatta gtcagctatc
    18961 gcgattatct ttattctacc cccgctgaac ttgttgattt tacacaaggt aagattctcc
    19021 ttcagcaggt aagaactcaa tatcagcatg aattcaacaa tactacgcat tcagaaaata
    19081 aagcatcttt tgattctatt tggcagcgtc tgacaaatca tgaagtcaca ccgcaacagc
    19141 atcccattgg ttttgtttta ggtgggcaac ccggagcagg taaatcatct ctgattgaat
    19201 tagccaaaag agagacaaaa gataatataa tgattattaa cggtgatgat tttcgatttc
    19261 tacaccctga ttttaattat atctaccaaa attatgggga tgattttgtt acccatacag
    19321 cgaaattttc cggcgaaacg gtcgaacgtg caattgagag agcgatagtc agtaaactca
    19381 acattgttgt cgaaggaaca tttcgtaatg ccgcgacgcc gttacaaaca ttaaaaaaac
    19441 tcaaagatgc aggctaccaa acagaagtca tgataaaaac aacctcagcg gcattaagct
    19501 gggaaagcac caatgaacgg tatagtaagg acaaagaggc gggaaatatt gcccgaaaag
    19561 tggataaaaa ccaccatgat atcgtcaccg gtttgctggc agaaaatgcc cgcaaggtgt
    19621 ttgcttcgaa cctgtcagat aagtttgcgg tgtattcgcg tgagaagatg atatttagtt
    19681 cacaagcggc aacgaatgac gatattgcaa cacttataca gaatgaaatc agcgggaata
    19741 cacaataata ataagagctg atggcgtgaa ataacgccgg gctttatgcc cggcgcatta
    19801 ccgtctggta cattaagcta tccagttcct taacgtcccc ttctctgcta tccggtaaca
    19861 gcggtggaat ttccccacgc gccagttccg cacgaataaa cgcatgtaac aatggacggc
    19921 gcggaccata ttccgcttct cctgcgcgct gtttacgttc cagtaattcg tctatttctg
    19981 cacgtaacgg cgcatcaagt tcgcttcccg ccagtaactc agcaaagcgc attggcggta
    20041 cgcctttacc cgcttcgacc cagcgcacgg ccagcagcgg acgcagcacg taaaagtatt
    20101 ttttcagtcg aacttcgtcg ccctgcaaat atccggggaa gtttttctgc gccatcgaat
    20161 agtaatgcca gcgcgcacgc agcggagaaa accacattgg gaccattgcc ttaaacgccg
    20221 ttatcgtttc ttcatcttgt tgataaatca ccggagaatc cagccattct atcagcgtcg
    20281 ggttcgcgcc tttcaataat cccagcgcct tacgccactc ccaaccggaa acatctaact
    20341 cgtcgtcgat gggcagctca ataacatcgc gcggtgactc tactcgcaga taccattcca
    20401 gcgggtgaac atacaaaaac cgtacatcgt agtcgctgtc gggcgaggca aatccccagc
    20461 cgcggctccc tgattcacag gcgtacagta ctttcacatc ataccgccgt tcaatttctt
    20521 ttaactgctg cgataccctt tcgcgcatcg cagcactgac tccatttaat ggcatgttac
    20581 tcaccctttg atattaacca acccatgcag aacctgatcc agaccgccaa atacagagat
    20641 gttatcgatc cgctcagcca ctctctccag cgtttctagc tcttttaaac gcaacgctac
    20701 cggattgttc tccatgactt tggcggtgtt cagtaacgag cgcgtcgctg ccgtttcttc
    20761 acgtcggcga atcacattcg cctgcgcaga tttctctgcc tcaaccagct gcgcgaggat
    20821 atttttcata tcaccaggca ggacgatatc tttcacccct aatgaggcaa tctccatacc
    20881 aaatggcaac atgcgcgatt tcacctgttc gctgacaacg tcatcaatga cctgtttatc
    20941 ttccagcagt tcatccagcg tacgagtacc taccgcttca cgcagggcaa attgcaattc
    21001 ccgatacaaa tgatcaatcg gcttcgttaa ctgactaaat gccagcaaca cgtcgctgta
    21061 gcgccagtta gcggcgaggt taatccgcag attgacctta tctttcgtta agatttcttg
    21121 cccgctgact tccagtacct gcaagcgggt gtcaaccact tcagcgtcaa ccagatggtt
    21181 aattttccag tatgcggtca gccccggcgg taacagcgcc tgcgtttcac catcgatttt
    21241 cagcacgcct acatgccagg ccggaacctg caccgttagt attgcatcgc ggcctttaac
    21301 tgcccctttg cgccgtggtt gcaataccgc attcatcacc tccgtttgta cctggacttg
    21361 ctgcgtattc atccgcacca gtgtcaaatc atcctctaca cgccagtaca aacggcgcgt
    21421 tgatggcggc aggatctcct gcaaaatacc gtccatatac aacgccccgg cttcaatctc
    21481 cgacaagtct gccaccaggc aatatttctc cacccaatca ggctgaaagc ggcgcaaata
    21541 atccgccagc acgtcgggaa cttcgctgcc atcgagcgta atcagcaaga cttctggcgt
    21601 atttaaccag ggtaaaagat gctcgcctgc atgtagcact ttgtagtaat cgccgtttct
    21661 ggagagtaat gccagctgat cttttcttac gcttattttt ttaatcatta tctattcctt
    21721 gcttaacagc cgtcgcggaa gcagacacga agcgacaggg gaattccctt ttgcctgtta
    21781 cccggcatct ttatcggcga cgttat
//
LOCUS       DAZGVQ010000082        21709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000082 DAZGVQ010000000
VERSION     DAZGVQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.083"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            282..1796
                     /gene="caiT"
                     /locus_tag="ACHLAG_002989"
     CDS             282..1796
                     /gene="caiT"
                     /locus_tag="ACHLAG_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFP0656175.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1827..2969
                     /gene="caiA"
                     /locus_tag="ACHLAG_002990"
     CDS             1827..2969
                     /gene="caiA"
                     /locus_tag="ACHLAG_002990"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFP0656176.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            3098..4315
                     /gene="caiB"
                     /locus_tag="ACHLAG_002991"
     CDS             3098..4315
                     /gene="caiB"
                     /locus_tag="ACHLAG_002991"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFP0656177.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4389..5942
                     /gene="caiC"
                     /locus_tag="ACHLAG_002992"
     CDS             4389..5942
                     /gene="caiC"
                     /locus_tag="ACHLAG_002992"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFP0656178.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            6051..6836
                     /gene="caiD"
                     /locus_tag="ACHLAG_002993"
     CDS             6051..6836
                     /gene="caiD"
                     /locus_tag="ACHLAG_002993"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFP0656179.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6842..7432
                     /gene="caiE"
                     /locus_tag="ACHLAG_002994"
     CDS             6842..7432
                     /gene="caiE"
                     /locus_tag="ACHLAG_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFP0656180.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVCSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7518..7913)
                     /gene="caiF"
                     /locus_tag="ACHLAG_002995"
     CDS             complement(7518..7913)
                     /gene="caiF"
                     /locus_tag="ACHLAG_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFP0656181.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8174..11395)
                     /gene="carB"
                     /locus_tag="ACHLAG_002996"
     CDS             complement(8174..11395)
                     /gene="carB"
                     /locus_tag="ACHLAG_002996"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFP0656182.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNADFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGFETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11413..12561)
                     /gene="carA"
                     /locus_tag="ACHLAG_002997"
     CDS             complement(11413..12561)
                     /gene="carA"
                     /locus_tag="ACHLAG_002997"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFP0656183.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(13017..13838)
                     /gene="dapB"
                     /locus_tag="ACHLAG_002998"
     CDS             complement(13017..13838)
                     /gene="dapB"
                     /locus_tag="ACHLAG_002998"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFP0656184.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            complement(14005..14919)
                     /gene="rihC"
                     /locus_tag="ACHLAG_002999"
     CDS             complement(14005..14919)
                     /gene="rihC"
                     /locus_tag="ACHLAG_002999"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFP0656185.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     ALLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLSTLPQLNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            complement(14985..15935)
                     /gene="ispH"
                     /locus_tag="ACHLAG_003000"
                     /gene_synonym="lytB"
     CDS             complement(14985..15935)
                     /gene="ispH"
                     /locus_tag="ACHLAG_003000"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFP0656186.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(15937..16386)
                     /gene="fkpB"
                     /locus_tag="ACHLAG_003001"
     CDS             complement(15937..16386)
                     /gene="fkpB"
                     /locus_tag="ACHLAG_003001"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP0656187.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(16474..16968)
                     /gene="lspA"
                     /locus_tag="ACHLAG_003002"
     CDS             complement(16474..16968)
                     /gene="lspA"
                     /locus_tag="ACHLAG_003002"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFP0656188.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(16968..19784)
                     /gene="ileS"
                     /locus_tag="ACHLAG_003003"
     CDS             complement(16968..19784)
                     /gene="ileS"
                     /locus_tag="ACHLAG_003003"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFP0656189.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(19827..20768)
                     /gene="ribF"
                     /locus_tag="ACHLAG_003004"
     CDS             complement(19827..20768)
                     /gene="ribF"
                     /locus_tag="ACHLAG_003004"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFP0656190.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(20776..20994)
                     /gene="yaaY"
                     /locus_tag="ACHLAG_003005"
     CDS             complement(20776..20994)
                     /gene="yaaY"
                     /locus_tag="ACHLAG_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFP0656191.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            21097..21360
                     /gene="rpsT"
                     /locus_tag="ACHLAG_003006"
     CDS             21097..21360
                     /gene="rpsT"
                     /locus_tag="ACHLAG_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFP0656192.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         5196 a         5825 c         5557 g         5131 t
ORIGIN      
        1 atattgcttt atggatcacc aatattgaaa gtcagatgct atttttttac tcacaacaca
       61 acataacaaa ctgattaata atcaaaatta acgagaaaat cgccctctat tcacccgaaa
      121 caaaaatgtg ataacaatca cagaatgcag cttattgaat acccattatg agttagccat
      181 taacgcgtcc acgaggttaa taataattat attaaatgtt aacaaaaata aaacaaacgg
      241 gaacgcaaaa taaatattcg ttttcacagt ggaattaact catgaagaat gaaaagagaa
      301 aaacgggaat agaaccgaag gttttctttc cgccgttaat aatcgtcggc atactttgtt
      361 ggcttacagt cagagatctg gatgcagcga atgtcgttat taatgctgta ttcagttacg
      421 tcaccaatgt atggggatgg gcatttgaat ggtatatggt ggtgatgctt ttcggttggt
      481 tctggctggt gtttggcccg tatgccaaaa agcgtttagg taacgaacca cctgaattta
      541 gtaccgccag ttggatcttt atgatgttcg cctcctgtac gtctgctgcc gtactgttct
      601 ggggatcgat tgagatctac tactacatct ccaccccgcc gtttggctta gaaccgaact
      661 cgacaggagc gaaagagttg gggctggctt acagcttgtt ccactgggga cctctgccgt
      721 gggccactta cagcttcctt tcagtcgcct tcgcttactt cttctttgtc cgcaaaatgg
      781 aagtgattcg ccccagctcg acactggtgc cgctggtagg tgaaaaacac gccaaagggt
      841 tgttcggcac tatcgtcgac aacttctatc tcgtcgcctt gatcttcgcg atgggtacca
      901 gtctgggcct tgccacgccg ctggtgaccg agtgtatgca atggttgttt ggcattccgc
      961 ataccctgca actggacgct atcatcatta cctgctggat tatcctcaac gccatttgcg
     1021 tcgcttgcgg tctgcaaaaa ggggtacgta tcgccagtga cgtgcgtagt tacctgagct
     1081 tcctgatgct gggttgggtg ttcattgtca gcggtgccag cttcatcatg aactacttca
     1141 ccgattcggt ggggatgttg ctgatgtatc tgccgcgcat gttgttctat accgatccca
     1201 tcgctaaagg cggcttcccg cagggctgga ccgtgttcta ctgggcatgg tgggtgattt
     1261 atgctatcca gatgagtatc ttcctcgccc gcatctcccg tggtcgtact gtgcgtgaac
     1321 tgtgcttcgg catggtgctg gggctgacag cgtcaacctg gatcctgtgg actgtactcg
     1381 gtagtaacac tctgctgttg atagataaaa acatcatcaa cattccaaat ctgatcgaac
     1441 agtacggtgt ggcgcgcgcc attatcgaaa cctgggccgc tctgccactc agcaccgcca
     1501 ccatgtgggg cttcttcatc ctctgcttta ttgccaccgt tacgctggtt aacgcctgct
     1561 cttataccct ggcgatgtcc acttgccgcg aagtacgcga tggtgaagaa ccaccgctgc
     1621 tggtgcgtat cggttggtca attctggttg gcattatcgg tattgttttg ctggcgctcg
     1681 gcggcctgaa accgattcaa actgccatta tcgccggagg atgcccgctg ttcttcgtca
     1741 acattatggt gacgctctcc tttattaaag acgcgaaaca gaactggaaa gattaattaa
     1801 cccccaaaat atcaagaggt tgaaagatgg attttaattt aaatgatgag caggaactgt
     1861 ttgtcgccgg tatccgcgaa ctgatggcca gcgaaaactg ggaggcctat tttgccgagt
     1921 gcgaccgtga cagcgtctac ccggaacgtt ttgtcaaagc actggcggat atgggtatcg
     1981 acagtctgct gatccctgaa gagcacggtg gtctggacgc ggggtttgtt actctcgccg
     2041 ccgtgtggat ggagctggga cgtctggggg caccaaccta tgtgctgtac cagttgccgg
     2101 gcgggttcaa caccttcctg cgcgaaggca cacaagagca gatcgacaag attatggctt
     2161 tccgcggcac cggtaagcag atgtggaact cagcgattac cgaaccgggc gcgggctccg
     2221 acgtgggtag cctgaaaacg acttataccc gtagaaatgg taagatttat cttaatggta
     2281 gtaagtgttt tattaccagc agcgcctaca ccccgtacat cgtggtgatg gcgcgcgacg
     2341 gggcttctcc ggacaaacct gtctacaccg aatggtttgt tgatatgagc aaaccgggca
     2401 tcaaagtgac caaacttgaa aagctcggtc tgcgtatgga tagctgctgt gaaatcacct
     2461 ttgacgacgt ggaactggac gagaaagaca tgttcggtcg ggaaggtaac ggctttaacc
     2521 gcgtcaaaga agagttcgat catgaacgtt tcctggtagc tctcaccaac tacggtacgg
     2581 cgatgtgcgc ctttgaagat gcggcgcgct acgccaacca gcgcgtgcag tttggcgagg
     2641 ctattggccg tttccagttg attcaggaaa aattcgccca catggcgatc aaattaaact
     2701 ccatgaaaaa catgctgtat gaagcagcgt ggaaagcaga caacggcacc atcacctctg
     2761 gcgatgcagc gatgtgcaaa tacttctgcg ccaatgcggc atttgaagtg gtggatagcg
     2821 caatgcaggt gctgggcggt gtcgggattg cgggcaacca ccgcatcagc cgcttctggc
     2881 gtgacctgcg tgtagaccgc gtctccgggg gatctgacga aatgcagatc ctgacgctgg
     2941 gtcgcgcggt gctgaagcaa taccgctaag tccgtgcgct gcctgatgcg acgctaatgc
     3001 gtcttatcag gcctacggga gcacgaatgt aggccggata aagcgtttac gccgcatccg
     3061 gcagtcatgc gctacataaa tttttcagga gctaataatg gatcatctac ccatgccgaa
     3121 attcgggccg ttggccggat tgcgcgttgt cttctccggt atcgaaatcg ccgggccgtt
     3181 cgccgggcaa atgttcgcag aatggggtgc ggaagttatc tggatcgaga acgtcgcctg
     3241 ggccgacacc attcgcgttc aaccgaacta cccgcagctc tcccgccgca atttgcacgc
     3301 gctgtcgtta aatattttca aagatgaagg ccgcgaagcg tttctgaaat taatggaaac
     3361 caccgatatc ttcatcgaag ccagtaaagg tccggccttt gcccgtcgtg gcattaccga
     3421 tgaagtactg tggcagcaca acccgaaact ggttatcgct cacctgtccg gttttggtca
     3481 gtacggcacc gaggagtaca ccaatcttcc ggcctataac actatcgccc aggcctttag
     3541 tggttacctg attcagaacg gtgatgttga ccagccaatg cctgccttcc cgtataccgc
     3601 cgattacttt tctggcctga ccgccaccac ggcggcgctg gcagcactgc ataaagtgcg
     3661 tgaaaccggt aaaggcgaaa gtatcgacat cgccatgtat gaagtgatgc tgcgtatggg
     3721 ccagtacttc atgatggatt acttcaacgg cggcgaaatg tgcccgcgca tgagcaaagg
     3781 taaagatccc tactacgccg gttgcggtct gtataaatgc gccgacggct acatcgtgat
     3841 ggaactggtg ggcattaccc aaattgaaga gtgctttaaa gatattggcc tcgcacatct
     3901 actcggtacg ccggaaatcc cggaaggcac tcagcttatc caccgtatcg aatgccctta
     3961 cggcccactg gtagaagaaa aactcgatgc ctggctggcg gcacatacca tcgcagaagt
     4021 taaagaacgc tttgccgaac tgaatatcgc ctgtgccaaa gtactgaccg taccggaact
     4081 ggaaagcaat ccacagtatg ttgcccgtga atcaatcact cagtggcaaa cgatggatgg
     4141 tcgcacctgc aaagggccga acatcatgcc gaaattcaaa aataaccccg gacaaatctg
     4201 gcgcggaatg ccctcacatg gcatggacac ggctgccatt ttgaaaaata tcggctacag
     4261 cgaaaacgac attcaggagt tggtcagcaa aggtctggcc aaagttgagg actaaacgcg
     4321 tggcgtcagt cggcttgcct gactagcgcc cgcccaataa caaacgaaca aaaatggata
     4381 gaggtgcaat ggatatcatt ggcggacaac atctacgtca aatgtgggac gatcttgcgg
     4441 acgtttacgg tcataaaacg gcgctgattt gtgaatccag cggcggagtc gttaaccggt
     4501 atagttatct tgagttaaat caggagatta accgcacggc aaacctgttt tatacgctag
     4561 ggattcgcaa aggcgacaag gttgctctac atctcgacaa ctgcccggaa tttatctttt
     4621 gctggttcgg gctggcaaaa attggcgcga ttatggtgcc gattaacgcc cgcctgttgc
     4681 gcgaggaaag cgcgtggatc ctgcaaaata gccaggcgtg cctgctggtg accagtgcgc
     4741 aattctatcc tatgtatcaa cagattcagc aggaagatgc cactcaattg cggcacattt
     4801 gcctgacaga tgtggcactt cccgctgatg atggcgtgag ttcgtttact caactgaaaa
     4861 atcaacaacc tgccaccttg tgctatgcac cgccgctatc gactgacgat acggcggaaa
     4921 ttctcttcac ctccggcacc acctcccgac cgaaaggtgt ggtgattacc cattacaacc
     4981 tgcgcttcgc tggatattac tccgcctggc agtgtgcact gcgtgacgat gacgtctacc
     5041 tgacggtaat gcctgcgttt catatcgatt gccagtgtac tgcggcgatg gcggcgtttt
     5101 ctgccggggc cacctttgtg ctggtcgaga aatacagcgc ccgcgccttc tggggacagg
     5161 tgcagaagta ccgcgccacc attaccgaat gtattccgat gatgatccgt acgttgatgg
     5221 tgcagccgcc ttcagcgaac gatcggcaac accgcctgcg ggaagtgatg ttttatctca
     5281 acttgtcgga gcaggaaaaa gatgcgtttt gtgaacgctt cggcgttcgc ttgctgacgt
     5341 cttatgggat gacggaaacc attgtgggca ttatcggcga tcgccctggc gataaacgac
     5401 gctggccgtc gattggtcgg gcggggtttt gctacgaagc ggagatccgc gacgatcaca
     5461 atcgcccgct cccggcaggt gagatcggtg aaatctgtat taaaggcgta ccagggaaaa
     5521 ccatcttcaa agagtatttt ctcaacccga aagccactgc gaaagtgctg gaagccgacg
     5581 gctggctaca taccggcgat accggatact gcgacgaaga gggctttttt tatttcgtcg
     5641 atcgccgctg caatatgatc aaacgcggtg gcgagaatgt ctcctgcgtg gagctggaaa
     5701 atatcatcgc cacccatcca aaaattcagg atatcgtggt tgtgggtatt aaagattcga
     5761 ttcgcgatga agccatcaaa gcatttgtgg tgctgaatga aggtgaaaca ttgagcgaag
     5821 aggaattttt ccgcttctgc gaacaaaata tggcgaaatt taaagtgccc tcttatctgg
     5881 agatcagaaa agatctgcca cgtaattgct cggggaaaat aattagaaag aatctgaaat
     5941 aaatgaaaca gcagggaact accctgccag ctaataatca cgctataaaa caatatgcat
     6001 tttttgcagg gatgttgtcg tccctgaaaa agcaaaaatg gagaaaagga atgagtgaat
     6061 cattacatct gacccgcaat ggatcaattc tggaaattac ccttgatcgt ccaaaagcga
     6121 atgctattga tgcaaaaacc agctttgaaa tgggcgaagt atttctaaat ttccgtgacg
     6181 atccgcaatt acgtgtcgcc attattaccg gtgccggaga gaagttcttt tccgcgggct
     6241 gggatttaaa agcggcagca gaaggcgaag caccggatgc tgactttggc ccgggtggat
     6301 ttgcgggatt aaccgaaatt ttcaatctcg acaaaccggt tatcgcagct gtgaacggct
     6361 atgcctttgg cggcggtttc gaactggcgc tggcggcaga ttttattgtt tgtgccgata
     6421 acgccagctt cgccctgccg gaagccaagc tgggcatcgt tcctgacagc ggcggcgtgc
     6481 tgcgtctacc gaagatcctg ccgcctgcca tcgtcaatga aatggtgatg accggcagac
     6541 gaatgggcgc agaagaggcg ctgcgttggg ggatagtcaa ccgcgtggtt agccaggcgg
     6601 aactgatgga taacgcccgc gaactggcgc agcagctggt taacagcgcc ccgctggcga
     6661 ttgcggcgct gaaagagatc ttccgcacca ccagcgaaat gccggtagaa gaagcgtatc
     6721 gctatattcg cagcggcgtg ttgaaacact atccgtcggt tctgcattcg gaggatgcca
     6781 ttgaagggcc gctggcgttt gccgagaagc gcgatccggt gtggaaagga cgttaacaac
     6841 cgtgagctat tacgcctttg aggggttaat tccggtggtt cacccgacgg cgtttgtcca
     6901 tcccagtgcc gtcttgattg gcgatgtgat tgtgggagcc ggtgtctaca tcggcccact
     6961 cgcctcactg cgtggtgact acgggcggtt gatcgtgcaa gcgggagcca atattcagga
     7021 tggctgcatt atgcatggct actgcgacac tgacaccatc gttggggaaa acggccatat
     7081 cgggcacgga gcgatcctgc atggttgtgt gattggtcgc gatgcattgg tcgggatgaa
     7141 cagcgtgatt atggatggcg cggtcattgg cgaagagagc attgttgccg ccatgagctt
     7201 tgtcaaagca ggctttcacg gcgagaaacg ccagttgttg atgggtacgc cagcccgcgc
     7261 tgtatgcagt gttagtgacg acgagttaca ctggaaacgg ttgaatacca aagagtatca
     7321 ggatcttgtc gggcgctgtc atgcatcgtt acatgaaaca cagccactga ggcaaatgga
     7381 ggaaaatcgc ccccgtttgc aggggacgac ggatgtgacg cctaaacggt gatcttgccg
     7441 gatgcgacct ggttgctgga taagatgcta caacatcgca tctggcaagc aggacgcccg
     7501 gtatcaacga gataaaatta acgacgcata ctctttgaca acatcatctg ccacttctgc
     7561 tcgcggttaa gttccgtgag cggaacggca ggaatacgcg gcgtctttct tacgccgact
     7621 aatttatccc ggctgttatt gcgcaggcgc gcgtaaattt gctcatcgat atcgaccact
     7681 tttaccagtc gctggcactg gcatccccgt ccttccagct tattagggat catcttaact
     7741 tcgcagctta tttctgcgac ttccgacaga atataagtca gggtattaac cgccttgctg
     7801 tgttcaatat cgaaatgaat agagatctct cttgctatca cccaccgatt ttcagccatc
     7861 atccattcgg cgattaacaa gtagagtggt ttttcaacat atccttcaca catacgaact
     7921 ccagtttcac aaattaaaag tctggctatg actcgttttg aagcaattaa acaaggccat
     7981 attaaaattg tggatatatc gatagtatgc aacttcaccg tcgataagtg tgagctaaat
     8041 cctgcttatg tcatcaaaaa tatcgattcc ccaaaagatt tacttaatgg gcaaaaaaaa
     8101 cggtcctcat cagagaaccg tattacttat tcgatcaaat tagcggatga aaaatatccg
     8161 ccatgacacg ctattatttg atctgcgcgt gcatttcctg caccgaaatt actttttcgg
     8221 tcgcgtcggc attcagcgcc atcgcggtag cgaaaccacc gttcagggtg gtgtcataat
     8281 gcactttata ttgcagcgca ctgcgacgga tcacgcggga gtcttcaatc gcgcgacggc
     8341 ctgaagtggt gttgatgatg taggtatatt cgccattctt gatgcggtcc tgaatgtgcg
     8401 gacggccttc atgcaccttg ttcaccagac gcggattaat gcccgcttcg cccagcacaa
     8461 tcgccgtgcc gtgggtcgca tccagctcga agccctgttt cagcagtttt gccgccaggt
     8521 ccaccacgcg ttctttatcg ccttcgcgca cggaaagcaa cgcacgaccg tgtttcttca
     8581 tggtggagtt gctgcccagc tgcgctttgg caaacgcttc agcgaaggtg cggcccacgc
     8641 ccatgacttc cccggtagag cgcatttctg gccctaacag cgggtcaaca cccgggaatt
     8701 tattgaacgg cagcaccact tctttcaccg agtagtacgg cgggataact tctttggtta
     8761 cgccctgctc agccagcgat ttgccagcca tcacacgcgc cgccactttt gccagcggaa
     8821 cgccggtggc tttggagacg aacggaacgg tacgcgccgc acgcgggtta acttcaatca
     8881 ggtagacttc gttgtttttc accgcaaact gcacgttcat cagaccgcgc acctgcaatt
     8941 cgaaggccag tttctgcacc tgctggcgca tcacatcctg aatttcctga cttaaggtgt
     9001 aggctggcag agaacatgcg gagtcaccgg agtgcacgcc cgcctgctcg atgtgctcca
     9061 taatgccgcc aatcagcacc atttcaccgt cgcagatggc atccacgtca acttccaccg
     9121 cgtcatcgag gaagtgatcc agcaacactg gcgcatcgtt agacacgctg accgccgtct
     9181 ggaagtagcg acgcaggtca gcttcgtcat agacgatttc catcgcccga ccgccgagaa
     9241 cgtaagacgg acgtaccacc agcgggtagc caatctcttt cgccttctct accgccattt
     9301 caatagcggt aacggtggcg ttcgccggtt gtttcagttt cagacgctca accgcatgct
     9361 ggaagcgttc acggtcttct gcacggtcga tagcatccgg gctggtgccg ataaccggta
     9421 cgccagcggc ttccagtgca cgcgccagtt tcagcggggt ctgaccgccg tactgaacga
     9481 taacgccttt cggcttctca atacgcacga tttccagcac atcttccaga gttaccggct
     9541 cgaagtagag gcgatcggag gtgtcgtagt cggtagagac ggtttccggg ttacagttaa
     9601 ccataatggt ctcaaaaccg tcttcacgca gcgccagcga ggcgtgtacg cagcagtagt
     9661 cgaattcgat accctgaccg atacggtttg gaccgccgcc aagcaccatg attttttcac
     9721 ggtcggtaga cggattcgct tcgcactctt cttcataagt ggagtacatg taagcggtgt
     9781 cggtggcaaa ctctgccgca caggtatcca cacgcttgta gaccgggtgc aggtcatatt
     9841 ggtcacgcag cttacggatt tccgcttcgc gtacacccgc gagttttgcc agacgcgcat
     9901 cggcaaagcc tttgcgtttc agctggcgca ggaagtcagc gttcaggcca gtgatgccca
     9961 cttccgccac tttctcttcc agacgcacca gttcttcaat ctgtaccagg aaccagcggt
    10021 caatgttggt caggttgaag acgccgtcca cagacaggcc cgcacggaaa gcatcggcga
    10081 tgtaccagat acgctctgcg cctgcgtctt tcagttcgcg acggattttg gttaacgctt
    10141 ccgggtcatc gaggctcact ttcgggtcga atccagtcgc accgacttcc aggccgcgca
    10201 gcgctttttg cagggattcc tgctgcgtgc gaccaatcgc catcacttcg ccaaccgatt
    10261 tcatctgagt ggtcagacgg tcgttagcac cagcgaattt ttcgaagttg aagcgaggaa
    10321 ttttagtaac cacatagtcg atggacggct cgaaggaggc cggagtacgt ccgccagtga
    10381 tgtcgttcat cagttcgtcg agggtgtaac ccaccgccag tttcgccgcc actttagcaa
    10441 tcgggaaacc ggtcgctttc gacgccagcg ccgaagaacg ggacacgcgt gggttcattt
    10501 cgataacaat caggcgaccg tttttcgggt tcaccgcaaa ctggacattg gaaccaccgg
    10561 tttcaacgcc gatttcacgc agcaccgcca tcgaggcgtt acgcatgatt tgatattctt
    10621 tgtcggtcag cgtttgggct ggcgcgacag tgatggagtc accggtatgg atgcccatcg
    10681 catcaaagtt ttcgatagag cagacgatga tgcagttgtc gtttttatca cgcaccactt
    10741 ccatctcgta ctctttccag ccgatcagcg actcatcaat cagcagctct ttggttgggg
    10801 agagatccag accgcgggcg caaatttctt caaactcttc gcggttataa gcgataccgc
    10861 cgccgctacc gcccatggta aaggatgggc gaataatgca cgggaagccc acgtcagcgg
    10921 caaccgccag cgcttcttcc atcgtgtgtg cgataccgga acgcgcggtt tccagaccaa
    10981 ttttcttcat cgctacgtcg aaacgacggc ggtcttctgc tttatcaatc gcatcggcgg
    11041 tcgcaccaat catagtcacg ccgaactctt ccaacacgcc ctgacgctcc agctccagcg
    11101 cgcagttcag cgccgtctga ccgcccatcg ttggcagcac cgcgtccggg cgctcttttt
    11161 caataatctt gcgtactact tcccagtgaa tcggctcgat gtaggtcgca tcggccattt
    11221 ccgggtcggt catgatggtc gccgggttgg agttcaccag aatgacgcgg taaccctctt
    11281 cgcgcagggc tttacatgct tgcgcgccag agtagtcaaa ctcacacgcc tgaccgataa
    11341 caatcgggcc cgcacccaga atcaggatac tttttatatc tgtacgtttt ggcatggctc
    11401 ttttactcct gattacttag cggttttacg gtactgctca attaactcga taaagtggtc
    11461 gaacaacggc gctgcgtcgt gtggcccagg gctggcttcc gggtgaccct ggaagctgaa
    11521 cgccggttta tcagtgcgat gaatgccctg taacgtaccg tcgaacaggg atttatgcgt
    11581 gacacgcagg tttgcaggta atgttgcttc atccaccgca aaaccgtggt tctgggcggt
    11641 gatcatcacc acgtttttct ccacatcttt aaccggatgg ttgccgccgt ggtgaccaaa
    11701 tttcattttg acagtcttcg caccgctcgc cagcgccagc agctgatgac cgaggcagat
    11761 gccgaatacc ggaatatcgg tttcgaggaa tttctggatg gcggtaatgg cgtaatcgca
    11821 cggggccggg tcgccaggac cgttggagag gaagatgccg tctggattca ttttcagcac
    11881 atcttccgca gaagtttgcg ccggaacgat ggtcaggcga cagcctctat ccaccagcat
    11941 ccgcaggatg ttgcgcttgg caccaaaatc ataagccacg acgtggaacg gcagctcgtc
    12001 ttcttttttc gcttctggca ggccgccggt caacgtccag ctcccttgtg tccagctata
    12061 ggcttctgcg gtggtcactt cttttgccag atccatgcca ttcagacctg ggaacgcgcg
    12121 ggctttttct aacgccaacg ccgcatccgg gttatcgcct gcgataatgc agccattctg
    12181 tgcgcctttc tcgcgcagta aacgcgtcag cttacgggta tcgatatcgg caatcgccac
    12241 gatgttatgg cgcttcaggt aagaagaaag gtcttcggta ttacggaagt tgctggcaat
    12301 cagcggcaga tcgcgaatca ccagaccttg tgcatgtacc tgagaagatt cttcatcggc
    12361 gtcattagtg ccgacattgc caatatgggg ataagtaaga gtaacgattt gacgagaata
    12421 ggaaggatca gtgaggattt cttgataacc ggtcattgaa gtattgaaaa cgacttcccc
    12481 aaccgccgaa cctgttgccc ctatggcccg accgtgaaac tgggttccgt cttccagaac
    12541 caatagcgct gacttaatca aaacaccctc cagagaatat tcactcactt tatttgcata
    12601 ttaattcaca atgatgacgt aaatcaatgc aaatctgctt accgacgaat ttctggcaaa
    12661 cggcggcatt ctggagatat gggcgctaaa agtcaactta atggcgggat tttttatatt
    12721 attttgtggc tcatgcatgt tttacaggca ttaagcgaca aaaagatcat ataacctgtt
    12781 ctggaaaacg cttgcgcagc aagaagattg catgaaataa aagcgagcag ggaaaagtgg
    12841 agcaaatggt caaaatttac attaaaacaa caaaaaccga agacaaaaca tgtaattttt
    12901 ggcacttata gacaatttaa aaacataaca ctaaaaataa aagggccaaa aattaaagcc
    12961 ctttataatc aataaattaa atgtgttatt cttgcaccat aacaaatatt ttgtggttac
    13021 aaactattga gatcaagcac atctcgcata tcaaaaagac cactttcctt accactcaac
    13081 cacaaagccg atcttaccgc gccgttagca aatgtcatgc ggctggacgc cttatgggtg
    13141 atctccagac gctcgccaat atcggcaaac atcgcggtat gttcgccaac gatgtcacct
    13201 gcacgcacgg tggcaaaacc aatggtgcca ggcacacgtt caccggtgtg gccttcacga
    13261 ctgtagaccg cgcaatcttt cagatcttta tcaagggcgt gggcgatcgc ctctcccatt
    13321 gccagtgcgg tgcctgacgg cgcatcaact ttatgtctat gatgtgcttc aataatttcg
    13381 atatcggtgt agtcacccat cactttggct gctttctcca gcagcttaag catgacgtta
    13441 acgccaacgc taaaattggc ggcaaagaca atcgcaatat cggcggcggc gtcacgaatc
    13501 gcctgtttac cggcttcgtc aaaccccgta gtgccgatca ccatcccttt gccatgctgg
    13561 cgacaaaaag cgagatggtt cagcgtacct tccggacggg taaaatcgat aaacacatca
    13621 aaatcatctt ttaccgcatc gaggctgctt tgcacggtaa cgcctgtttt ccctgcacca
    13681 gccagctcac cggcgtcgct gcccagtaaa gaagatcctt cgcgctccag cgccgcgccc
    13741 agctgcacgc cttctaatgc cagcgccgcc tgaatcaact ggcggcccat acgccccccg
    13801 gctcccgcga tggcaacgcg gatgtttgca tcatgcatag ctattctctt ttgttaattt
    13861 gcatagaccg ttttcagagt aaccagcgca tcgctacgcc gccagccaaa aacgctgata
    13921 attagaaatt aatgataaac agggagggat atcagtgaaa ggcatgagtc gatgaatgac
    13981 tgcatgccaa taacatgtga caggttacga cgccagagcc agcacctcag ccacccactg
    14041 ctggaagcct ttcacatcca gatccaatgc cacctgtaca ttggctggct tgcccaggca
    14101 accgtcgata tcaaccaccg tcgtgcctga ggtaaattcg ccctgagttt ccactgccac
    14161 aaaacagggt ttgagagtga acaggtccgg gcgcaccagc caggcgatgg cgcagagatc
    14221 gtgcattcgc aagccgcttt gcatactgcc gctacggtag tggctaaaca gggcgtgaag
    14281 cattttcccg gtacggttta actgcggcag tgtagagaga tagtcaggag ttaatattgc
    14341 ctgattggtg acatccaaac cgcacatgac gatttcaata ccactgcgga agacacaggc
    14401 agcagcttct ggatcggcag caatattaaa ctcggcgttt ggcgtacagt tgccgcgtcc
    14461 ggcagaacca cccatgatca ccagacggcg aatatacggc ttgcattccg ggcattgtga
    14521 aagtaacagc gcaatattgg ttaacgggcc gatggccacc agggtaacag gctctggtgc
    14581 acgcatcagg gcatcccgaa tcgccagaag cgccggtatc ccgagcggct ttcggttgtg
    14641 ctcaacaaag tcgtagccag ccattcccga ttcgccgtgc acagatgccg catcacgcgg
    14701 tgcgcgtacc agcggcacag cggccccttg ggcgagcgga atctccgcat tccagaaatg
    14761 cagcagttgc agggcattgc gggtagtttt ctcaaccgag acattacccg cgacggtggt
    14821 catcagttgc aggtcgagtt cgggtgcaaa aatcgcggcg gcaatggcga cggcatcgtc
    14881 aatgccgggg tcagtatcga ggaagatagg taaacgcatg ttttctccat aaaaaatgcc
    14941 ggtaacaaga ccggcatttt cgcataactt aggctgctaa tgacttaatc gacttcacga
    15001 atatcgacac gcagctcttt cggcacttcg aaaacaatgt tttcttcacg gccttccagc
    15061 ggaatggctt caccaccgcc cagctgctgc aaacgtgcca ccacattctg caccagaata
    15121 tccggagccg atgcgcccgc agtcacgccg acgcatttaa cctctttcac ccactcttcc
    15181 tggatatctt tcgcatcgtc aatcaaaaac gcgcgtttgc ccatacgctg ggccagctcc
    15241 gccagacggt tggagttgga ggagtttttc gaaccgacca ccaacacaac ttccgcctgt
    15301 tctgccaggg cgcgtaccgc ttcctgacgg ttagtcgtgg cgtagcagat gtcatctttg
    15361 cgcggaccga caattttcgg gaagcgttta cgcagcgcgt cgatcacatc agacgtgtca
    15421 tccaccgaca gcgtggtctg ggtcataaag gagagcttct cttcgttttt gaccgtcagt
    15481 ttccacacat cgtccggcga ttcgaccaga tacattcccc cttccgggtt actgtactgg
    15541 cccattgtcc cttccacttc cgggtgcccg gcgtgaccga tgagaataga ttcttcgcca
    15601 cggcgactgg cgcgggcgac ttccatatgc actttggtca ccagcggaca ggtcgcgtca
    15661 aataccgtca aatcgcgact ttttgcttcg ttacgtaccg cctgagaaac accgtgtgcg
    15721 gagaaaatca ggatcgcgcc gtccggtact tcgctaatct gctcaataaa gatagcccca
    15781 cgctcacgca agctatcgac cacatagcgg ttatgtacca cttcgtgacg gacatatatc
    15841 ggtgcgccgt aaatggccag cgcgttttca acaatgctga tagcgcggtc taccccggca
    15901 caaaaaccac gcgggttggc caacaggatc tgcatgttac gcctccagtg ccggatcgat
    15961 ttccagcact tcaatatcaa aatgaacggt ctgcccggcc agcggatggt tgaaatcaac
    16021 ggtaatggag tcgccgttaa tttcgcggat cacgccaggc atctcactgc catccattgc
    16081 ggtaaaaagc atgattgcgc caatttctgg ctcgcctgca tccataaatt cacggcggga
    16141 gaagtactga atcaggtccg gtgacggcac gccaaacgcc gcatcgggct ccagcgagaa
    16201 ggtggtttta tcgcccactt tcagccccaa caggtgttgc tccagccctt cagaaagaga
    16261 agcatcgccc aggcggaaca gcgccggttt accgttgttg cgggtagact cagcggtggt
    16321 gccatcatcg agtttcagcg tgaagtgcac caggacggcg ctattgctct gtacagattc
    16381 agacatgcag gtttgctctt atattttgag gatttttgcc ggatgcggcc tacgatttcg
    16441 cagcaatctg taggccttat ccggcagggt ttattattgt tttttcgctt tagaaggcaa
    16501 aaaaccttcc agcacaatca gtgccgcacc gacacaaatg gcagtatcgg caaggttgaa
    16561 ggtggcgaag tgccagtcgc cgacgtagaa gtcgatcata tcgacaacga agccgtgcca
    16621 caggcggtcg aacaggttgc ccagcgcgcc gccaataatc agcgcataag cgatattgtt
    16681 tagcttctgc gtggccttcg agcgatacat catcactgcc aggatcacgc taataccaat
    16741 cgcaataccg gcaaagaacc aacgctgcca gccgccgcta tcggcaagga aactaaacgc
    16801 cgcgccatag ttacgcgcat aatgcagatt aagcgacggg aacagcggga ccgtatcccc
    16861 cagagcaaag ttctggagga tcaggtattt gctgcccaga tcgataatca gcacgactac
    16921 caccagccac agccagcgta gccctgttga acagatcgat tgactcatca ggcaaactta
    16981 cgtttttcac cgtcaccggc gacgttgctg acacagcggc cgcagatttc tgcgtgttcc
    17041 gccaccttgc cgacatcctg ggtgtagtgc cagcagcgtg ggcacttctc accttcggct
    17101 ttactcaacg cgactttcag ccctttgagt acttcgctct gctgagcatc agcaggtgcg
    17161 tcgttatagt ctgcaacggt agcgccggag gtcaacagga caaatcgtaa ttcatcgccc
    17221 agcgcggtca gtttcgccga cagttccggt tctgcataca aggttactgc cgcttccagc
    17281 gagccaccca ctttcttgtc ggcacgcgct tgctcaatga ctttgttcac ttcgccacgc
    17341 actttcaaca gctcgtccca gaacgcatcg ttcatcgctt cactgtctgc caggccaaac
    17401 aggccttcgt accactcacc ggtgaagacg tatttttcac gttcgcccgg caggtagccc
    17461 cacacttcat cagcggtgaa ggagaggatt ggtgccatcc agcgcaccag cgcttctgcg
    17521 atgtgataca gcgcagtctg gcagctacga cgcgccacac tgtccgcttt ggcggtgtac
    17581 tgacggtctt tgatgatgtc gaggtagaag gaacccatct caacggagca gaagcgcatc
    17641 agacgctgta ccacttcgtg gaaatcgtat gcttcgtacg ccttgaggat gtcttcctgt
    17701 gccgctttcg cacaacctac ggcccagcga tccagtacca ccatctcttc cggtttcacc
    17761 atatcttttg ctggatcaaa accgttcagg tttgccagca ggaagcgcgc ggtgttacgg
    17821 atacgacgat agctatcggc agcacgtttc aggatctcgt cagaaacggc catttcaccg
    17881 gtgtagtcgg ttgatgccac ccacagacgc agaatatccg cgcccagttt gttcatcaca
    17941 tcctgcggcg aaacggtatt gccgatggat ttagacatct tgcgaccctg accatccacg
    18001 gtaaagccgt gggtcagtac ctgacgatac ggcgctttgc ctttcatcgc ggtggagatc
    18061 attagggaag acatgaacca accacggtgc tggtcagaac cttccagata catgtccgct
    18121 gcgtgaccgg caaattccgg acgcacgtca acaacagaag agtgggtaga tccggagtca
    18181 aaccatacat ccaatgtgtc cggcactttc acgtactgat cagcttcatc gccgaggatc
    18241 tctttcgcat caagatccca ccacgcctgg atgccgtcaa cttcaacgcg ttttgccact
    18301 tcttccatta gttcgagagt acgcggatgc agctcttccg tgtctttgtg cacgaacagt
    18361 gacatcggta cgccccaggt gcgctgacgg gagatacacc agtcaggacg gttagcaacc
    18421 atcgactcga tacgcgcctg gccccagtcc gggatccact gcacgccttt gatctctttc
    18481 agtgactgcg cacgcagacc tttctgatcc atgctgacga accactgcgg cgtcgcgcgg
    18541 aagatgatcg gcgttttgtg acgccagcag cacggatagc tgtgctgcat tttctcaacg
    18601 tgcagcagcg cgcctttttc ctgcagcagc gcaacgacga tgtcgttcgc tttgaagacg
    18661 ttcacgccat ccagcgtcgg ataagtgccc ggcagataag tgccgtccgg gccaaccggg
    18721 ttagcggttt ccaggccgta tttctgaccg atcacatagt cgtccgggcc gtggccaggc
    18781 gcggtgtgaa cggcaccggt accggcatcc agggtaacgt gatcgccgag gattgccgga
    18841 acgtcgaagc ccataaacgg atgggtaaag cgcagcaact caagctccgc accttttacc
    18901 gtgccgagaa tggtgtaatc ggtcacgccg atacgctgca ttacgctttc aaccagatct
    18961 ttcgccagaa tcacggcctg accgtcaatc tgcaccagcg catagtcgaa atcaggtgca
    19021 atagagattg cgcggttcgc aggcagagtc cacggcgtag tggtccagat caccagcgag
    19081 attgggccgt taacgttgct tacggcaaat tttgctttca gtgcatcctg atcgaccgcc
    19141 tgaaaagcaa cgtcgatgga cggagaagtt ttgtcgtaat actcaacttc cgcttccgcc
    19201 agcgcagaac ggcagtcaac gcaccagtga actggcttcg cgcctttgtg caggtgaccg
    19261 ttgccgatga ttttgcccag cgcgcggatg atgttggctt cagttttgaa gtccatggtc
    19321 aggtacgggt gcgaccagtc gcccagcacg cccagacgga taaagtcttt gcgttgaccg
    19381 tcaacctggg tcgccgcgta ttcgcggcac ttggcgcgga actcggcggc ggtgaatttc
    19441 tcacccggct taccgtattc ttgctctact ttcagctcga tcggcagacc gtggcagtcc
    19501 cagccaggca catacggcga gtcataaccg gaaagccctt tggacttcac gataatgtct
    19561 ttcagaatct tgttaaccga gtgaccaata tgaatgctgc cattcgcata aggagggcca
    19621 tcatgcagaa tgaaggtttt tttgcctttt ttagccgcac ggatgatgcc gtacagatca
    19681 tcatcagtcc aacgcgccag cattcccggt tcgcgcttgg cgagatcgcc acgcatcggg
    19741 aaccctgttt ccggcaaatt cagggttgat ttatagtcac tcatcagatt ctcggttccg
    19801 tgtttcggtt tgattacata acaggcttaa gccggttttg ttagcccaaa aaattcgcgg
    19861 gcggttaatt catcacgcgc aatctgcgct ttcagttcgt ccagcgacgc aaatcgctgc
    19921 tcattgcgta tttttttacg cagcactact tgtatatggc gaccgtaaag gtccattgca
    19981 acatctaaca aatgcacttc cagctgctgg cgaataccgg caaccgttgg gcgtgttcca
    20041 atgtttgcca cgccgggtaa cggcttttca ccgaggccca gcacttctac cgcataaacc
    20101 cctttcaccg gggaaacctg acggcgtagc ggtacattcg ccgtcgggaa acctatagtg
    20161 cgccctaatt catcaccgtg gactacacgc ccggagatgg caaacgggtg ccccagtaaa
    20221 ctttctgcca gagccagatt gtcatccgcc agcgcctgac gcacagcggt gctgctgata
    20281 cgcacgccac cttcgcaaaa agtttgcgta ctggttatat cgaagccgta ttccatgcca
    20341 gctttctgta ataacaagaa atcgccttca cgaccagcgc caaagcggaa atcatcacct
    20401 acggcaagaa attttacgcg caaatgcttc accagaagat cgctgatgaa attttgcgcg
    20461 gttaacgccg cgaaacgcct gtcgaaacgc acgcacagca cgtaatcaac gccacactct
    20521 gcaaggtaac gcagtttttc ccgcagccgg gtcagccgtg ccggggcttt atcggtagca
    20581 aacagttcca gtggttgagg ttcaaaaagc atcaccatca ccggtaagtt gcgcttgcgc
    20641 ccttcttcct gcaagccctg taacagcgcg cgatgaccgc gatgcacgcc gtcgaaatta
    20701 ccaatagtca gcacacaccc ttcttgcggg gcctggctga gattatgtat gccgcgtatc
    20761 agcttcatgt ctggctcaaa acagtgaaaa tcgtccgagt ataccttgta cagcggtcaa
    20821 ggttaaccgg cgattgagta ccgaaatcag aaagaagtgg cttttcattg ccatggcgca
    20881 aatcacggga agaaagtgac cgcctgctgc aatttttatc gcggaaaagc tgtattcaca
    20941 ccccgcaagc tggtagaatc ctgcgccatc actacgtaac gagtgccggc acattaacgg
    21001 cgcttatttg cacaaatcca ttgacaaaag aaggctaaaa gggcatattc ctcggccttt
    21061 gaattgtcca tatagaacac atttgggagt tggaccttgg ctaatatcaa atcagctaag
    21121 aagcgcgcca ttcagtctga aaaggctcgt aagcacaacg caagccgtcg ctctatgatg
    21181 cgtactttca tcaagaaagt atacgcagct atcgaagctg gcgacaaagc tgctgcacag
    21241 aaagcattta acgaaatgca accgatcgtg gaccgtcagg ctgctaaagg tctgatccac
    21301 aaaaacaaag ctgcacgtca taaggctaac ctgactgcac agatcaacaa actggcttaa
    21361 tcgccaattt gctgaagctt tgtgaaaaag cccgcgcaag cgggtttttt tatgcctgct
    21421 gcttttatga tgcgtgagag aacacactac cggttgccag atagtcaaaa gatgctggct
    21481 tttgcagaca tgccgttaca ctttctgaac tcttcgtggc ttaagaatcg tagaaccatc
    21541 ggctgataca ttctgaacaa gaatatctac tcaatctcca gactccccta caaagaatgt
    21601 ggatattgtg atacacattg aggtattaaa cttaagacca ctctattttt gaattaaatt
    21661 cctatacact ttaaccattc ataaatatct ccttattaga tcttgtaaa
//
LOCUS       DAZGVQ010000083        21657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000083 DAZGVQ010000000
VERSION     DAZGVQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.084"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            76..747
                     /locus_tag="ACHLAG_003007"
     CDS             76..747
                     /locus_tag="ACHLAG_003007"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFP0656193.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            757..1953
                     /gene="yaiP"
                     /locus_tag="ACHLAG_003008"
     CDS             757..1953
                     /gene="yaiP"
                     /locus_tag="ACHLAG_003008"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP0656194.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            1931..2512
                     /locus_tag="ACHLAG_003009"
     CDS             1931..2512
                     /locus_tag="ACHLAG_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP0656195.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIESEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2514..3287
                     /gene="yaiO"
                     /locus_tag="ACHLAG_003010"
     CDS             2514..3287
                     /gene="yaiO"
                     /locus_tag="ACHLAG_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFP0656196.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3472..3747
                     /gene="frmR"
                     /locus_tag="ACHLAG_003011"
     CDS             3472..3747
                     /gene="frmR"
                     /locus_tag="ACHLAG_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFP0656197.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3782..4891
                     /gene="frmA"
                     /locus_tag="ACHLAG_003012"
     CDS             3782..4891
                     /gene="frmA"
                     /locus_tag="ACHLAG_003012"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFP0656198.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            4984..5817
                     /gene="fghA"
                     /locus_tag="ACHLAG_003013"
     CDS             4984..5817
                     /gene="fghA"
                     /locus_tag="ACHLAG_003013"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP0656199.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(5942..6481)
                     /gene="yaiL"
                     /locus_tag="ACHLAG_003014"
     CDS             complement(5942..6481)
                     /gene="yaiL"
                     /locus_tag="ACHLAG_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFP0656200.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6583..7794)
                     /gene="mhpT"
                     /locus_tag="ACHLAG_003015"
     CDS             complement(6583..7794)
                     /gene="mhpT"
                     /locus_tag="ACHLAG_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFP0656201.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKSILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8069..9082)
                     /gene="mhpE"
                     /locus_tag="ACHLAG_003016"
     CDS             complement(8069..9082)
                     /gene="mhpE"
                     /locus_tag="ACHLAG_003016"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFP0656202.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9079..10029)
                     /gene="mhpF"
                     /locus_tag="ACHLAG_003017"
     CDS             complement(9079..10029)
                     /gene="mhpF"
                     /locus_tag="ACHLAG_003017"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFP0656203.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10026..10835)
                     /gene="mhpD"
                     /locus_tag="ACHLAG_003018"
     CDS             complement(10026..10835)
                     /gene="mhpD"
                     /locus_tag="ACHLAG_003018"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFP0656204.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQA
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(10845..11711)
                     /gene="mhpC"
                     /locus_tag="ACHLAG_003019"
     CDS             complement(10845..11711)
                     /gene="mhpC"
                     /locus_tag="ACHLAG_003019"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFP0656205.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSMAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11729..12673)
                     /gene="mhpB"
                     /locus_tag="ACHLAG_003020"
     CDS             complement(11729..12673)
                     /gene="mhpB"
                     /locus_tag="ACHLAG_003020"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFP0656206.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFSQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAVKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12675..14339)
                     /gene="mhpA"
                     /locus_tag="ACHLAG_003021"
     CDS             complement(12675..14339)
                     /gene="mhpA"
                     /locus_tag="ACHLAG_003021"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFP0656207.1"
                     /translation="MAIQHPDIQPAVNHSVQVAISGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LNTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            14530..15363
                     /gene="mhpR"
                     /locus_tag="ACHLAG_003022"
     CDS             14530..15363
                     /gene="mhpR"
                     /locus_tag="ACHLAG_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFP0656208.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLAARPGDDYQLAREPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     H"
     gene            15440..16522
                     /gene="lacI"
                     /locus_tag="ACHLAG_003023"
     CDS             15440..16522
                     /gene="lacI"
                     /locus_tag="ACHLAG_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFP0656209.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQVQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16645..19719
                     /gene="lacZ"
                     /locus_tag="ACHLAG_003024"
     CDS             16645..19719
                     /gene="lacZ"
                     /locus_tag="ACHLAG_003024"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFP0656210.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPVADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSTFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEASDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHIIPQ
                     LTTSETDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19771..21024
                     /gene="lacY"
                     /locus_tag="ACHLAG_003025"
     CDS             19771..21024
                     /gene="lacY"
                     /locus_tag="ACHLAG_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFP0656211.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21090..>21657
                     /gene="lacA"
                     /locus_tag="ACHLAG_003026"
     CDS             21090..>21657
                     /gene="lacA"
                     /locus_tag="ACHLAG_003026"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFP0656212.1"
                     /translation="MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAAGVPCRVIREINDRD"
BASE COUNT         5077 a         5847 c         5612 g         5121 t
ORIGIN      
        1 tggctgcgcc agagattacg tctctgagtg catcgttgtg cttgtagtat aataaataaa
       61 atggagtgtc attaagtgga taaggtttta gattcagccc tcctttcttc ggcaaataaa
      121 agaaagggta tccttgctat tggcgcacat cctgacgata tagaattagg ctgtggcgca
      181 tcgcttgctc gtcttgcgca aaaaggaatt tatatcgcag cagtggtgat gactaccggc
      241 aactctggta cagatggaat aatcgatcgc catgaagaat cgcgcaacgc cctaaagata
      301 ttagggtgcc accaaactat tcatcttaat tttgctgaca cccgcgctca tttacagctc
      361 aatgatatga tttccgccct ggaagacatc attaaaaatc aaattccttc tgatgttgaa
      421 atcatgcggg tatataccat gcatgatgcc gaccgccatc aggatcatct cgctgtttat
      481 caagcttcaa tggttgcctg ccgcactatt ccacaaattc tcggctacga aaccccaagt
      541 acctggcttt catttatgcc tcaggttttt gaatccgtta aagaagaata tttcacagtc
      601 aaacttgctg cattaaaaaa acataaaagc caggaacgac gtgattatat gcgtcatgat
      661 cgcctgcgtg cagttgcaca atttcgcggg caacaggtca atagcgatct gggtgaaggc
      721 tttgtaattc ataaaatgat tctttgagca aattgcatga aaacctggat atttatctgt
      781 atgtccatag caatgttgct atggttttta agtacgctaa gacgtaaacc cagtcaaaag
      841 aaaggctgta ttgacgccat tatacctgcg tataacgaag gcccgtgtct ggcgcagtca
      901 ctggataatc tactgcgtaa cccttatttt tgccgggtaa tttgcgttaa cgacggctcc
      961 acggacaata ccgaagcggt catggcggaa gtcaaacgca aatggggcga ccgctttgtt
     1021 gccgtcacgc aaaaaaatac cggtaaaggt ggtgcgctga tgaatggcct caattatgcc
     1081 acctgcgacc aggttttttt aagtgatgcc gacacctatg ttccgcccga tcaagacgga
     1141 atgggctata tgctggcaga aattgagcgc ggtgctgatg ccgtaggcgg cattccctct
     1201 actgcgttga aaggcgcggg gctgttaccg cacatccgcg cgaccgtaaa gttgccgatg
     1261 attgttatga agcgcacgct acagcagctc ctgggcggcg caccgtttat tatcagcggt
     1321 gcctgcggga tgttccgtac tgatgtattg cgtaagttcg gtttctcgga tcgtactaaa
     1381 gtcgaagacc ttgatctcac ctggacatta gtggcaaacg gctaccgtat tcggcaggcg
     1441 aatcgctgca tcgtataccc acaggaatgc aacagtccgc gtgaggagtg gcgtcgctgg
     1501 cggcgttgga ttgtgggata cgcggtctgt atgcgcctgc ataaaagact tttatttagc
     1561 cgcttcggta tcttcagtat atttcctatg ctgttggttg tgatttatgg cgttgggatt
     1621 tatctcacta cctggtttaa tgaattcatc accaccgggc cgcatggtgt ggtgttggca
     1681 atgtttccgc ttatctggat cggcgtagtt tgtgttattg gtgcttttag cgcctggttt
     1741 catcgttgct ggttgttggt gcctttagcg ccgctttccg ttgtgtatgt attattagct
     1801 tatgccatct ggattattta tggacttatt gcctttttta ctggacgcga acctcagcgc
     1861 gacaaaccca cccgctattc cgcactggtg gaagcgtcaa ccgcttattc ccaaccttct
     1921 gtcacaggaa ctgaaaaact atctgaagct taatgttaaa gagaaaaata ttcagattgc
     1981 agaccaggta attattgatg aaactgcagg tgaagttgtt atcggcgcga atacccgtat
     2041 ttgtcatggt gccgttattc agggtccggt agtgattggc gcaaactgcc tgataggtaa
     2101 ttatgcgttt attcgtcctg gcacaataat cagcaatggt gtaaaaattg gttttgccac
     2161 cgaaattaaa aatgcggtta ttgaatcgga agcaacgatt ggtccgcaat gttttattgc
     2221 cgactcggta gttgcaaacc aggcatattt gggcgcacaa gtacgtacca gtaatcatcg
     2281 tctggatgaa cagcctgtgt ctgttcgaac tccagaggga attatcgcta ccggatgcga
     2341 taaattaggt tgttatatcg ggcagcgttc acgccttggc gtacaagtta taattttgcc
     2401 tgggcgaatt atttccccga acacgcaact tggcccgcgc gtgattgtag aacgtaattt
     2461 acctgccgga acttattcac tccgacaaga acttatccgt acaggagatt aaaatgataa
     2521 aacggacgtt attagcggcg gccattttta gcgcattgcc cgcttatgcc gggttaactt
     2581 ccattaccgc gggctacgat tttaccgatt attctggcga tcatggcaac cgtaatttag
     2641 cgtatgctga actggtggcg aaagttgaaa acgcaacgct gctttttaat ctttcccagg
     2701 ggcgtcgtga ttatgaaact gaacatttca atgccactcg cggtcagggt gcggtctggt
     2761 ataaatggaa taactggctg acaacccgaa cgggtattgc ctttgcggat aatacgccgg
     2821 tctttgcccg ccaggatttt cgtcaggata ttaacctggc cctgttgcca aaaacgcttt
     2881 tcacgaccgg ttatcgctac actaaatatt acgatgatgt cgaagtcgat gcctggcaag
     2941 gcggcgtatc actctatact ggcccggtga tcaccagcta ccgctatacc cattatgact
     3001 ccagcgatgc aggtggtagt tatagcaata tgatttccgt gcgtctgaat gacccgcgcg
     3061 gcactggtta tacgcaacta tggctaagcc gcggaacagg tgcttacacc tatgactgga
     3121 cgccagaaac acgctacggc agcatgaaga gcgtcagtct gcaacgtatt caaccgctga
     3181 ctgagcaact taatctcggg ctgacggcag gtaaagtgtg gtacgacacc ccaaccgatg
     3241 attataacgg tctgcaactt gcagcccatc tgacctggaa attctgattc cttctgccgc
     3301 ccgctatccg gggcggcctt ccctgccgat tagccccccc tttcctcttt gttttccgac
     3361 cacattcacc ggataaattt tattctccag tgttatatac tatagggggg tatgcattga
     3421 catatagcat acccccctat agtatattgc gtgcagataa tgaggtgcga aatgcccagt
     3481 actccggaag agaagaaaaa ggtccttact cgagttcgtc gtattcgagg gcagattgat
     3541 gctctggaac ggtcgctgga gggtgatgcc gaatgccgtg ccatactcca acagatcgct
     3601 gccgttcggg gcgcggctaa tgggctgatg gcagaagtgc ttgaaagcca tatccgggaa
     3661 acgtttgacc gaaatgactg ctacagccgc gaagtcagcc aatccgttga cgacactatt
     3721 gaactggttc gagcttatct taaatagctg aatctattac catattgagg aagagcgaga
     3781 gatgaaatca cgtgctgccg ttgcatttgc tcccggtaaa ccgctggaaa tcgttgaaat
     3841 tgacgttgca ccaccgaaaa aaggtgaagt gctgattaaa gtcacccata ccggcgtttg
     3901 ccataccgac gcatttaccc tctctgggga tgacccggaa ggtgtattcc cggtggttct
     3961 cggtcacgaa ggggccggcg ttgtggttga agtcggtgaa ggcgtaacca gcgtcaaacc
     4021 tggcgaccat gtgatcccgc tttacaccgc ggagtgcggc gagtgtgagt tctgtcgttc
     4081 tggcaaaact aacctctgtg ttgcggttcg cgaaacccag ggtaaaggct tgatgccaga
     4141 cggcaccacc cgtttttctt acaacgggca gccgctttat cactacatgg gatgctcaac
     4201 attcagtgaa tacaccgtgg tcgcggaagt gtctctggcc aaaattaatc cagaagcaaa
     4261 ccatgaacac gtctgcctgc tgggctgtgg cgtgaccacc ggtattggcg cggtgcacaa
     4321 cacagctaaa gtccagccag gtgattctgt tgccgtgttt ggtcttggcg cgattggtct
     4381 ggcagtggtt cagggcgcgc gtcaggcgaa agcgggacgg attatcgcta tcgataccaa
     4441 cccgaagaaa ttcgatctgg cgcgtcgctt cggtgctacc gactgcatta acccgaatga
     4501 ctacgacaaa ccgattaaag atgtcctgct ggatatcaac aaatggggta tcgaccatac
     4561 ctttgaatgc atcggtaacg tcaacgtgat gcgtgcggcg ctggaaagtg cgcaccgcgg
     4621 ctggggtcag tcggtgatca tcggggtggc aggttctggt caggaaatct ccacccgtcc
     4681 attccagttg gtcactggtc gcgtatggaa aggttccgcg tttggcggcg tgaaaggtcg
     4741 ttcccagtta ccgggtatgg ttgaagatgc gatgaaaggt gatatcgatc tggaaccgtt
     4801 tgtcacgcat accatgagcc ttgatgaaat taatgacgcc ttcgacctga tgcatgaagg
     4861 caaatccatt cgaaccgtaa ttcgttactg atttcccgca ggtatacccc gtccacttca
     4921 gacggggttc ttaatactct ccctgggcag ccgtccgggg gattaaccgt gagataatga
     4981 ctgatggaac tcattgaaaa acatgccagc tttggcggct ggcaaaatgt gtatcggcat
     5041 tattcccaat cactgaaatg tgaaatgaat gtcggcgtct atctcccacc aaaagccgcg
     5101 aatgaaaaat tgccggtgct gtactggctt tcgggcctga cctgcaacga gcagaatttc
     5161 attactaaat cggggatgca gcgttacgcg gctgaacaca acattattgt tgttgcgccg
     5221 gacaccagtc cgcgaggcag tcatgtcgca gatgccgacc gttacgatct cgggcaaggt
     5281 gccgggtttt acctgaacgc aacgcaagcg ccgtggaatg aacattacaa aatgtatgac
     5341 tatatccgca acgagctgcc ggatttagtg atgcaacatt ttccggcaac gaccagaaag
     5401 tctatctctg gtcattctat gggcgggctg ggcgcgctgg tgctggcgtt acgtaaccca
     5461 gatgaatatg tcagcgtctc ggcgttttcg cccattgtct ccccatcgca agtaccgtgg
     5521 ggacagcaag cctttgccgc atatcttggt gaaaataaag atgcctggtt gaattacgat
     5581 ccggtgagtc ttatttcaca aggtcaacgc gttgcggaaa tcatggttga tcaggggttg
     5641 agtgatgatt tttacgcgga acagctgcgg actccaaatc ttgaaaagat ctgccaggag
     5701 atgaatatca agacgttaat ccgttatcac gagggttacg accacagcta ttattttgtc
     5761 tccagtttta ttggcgagca tattgcctac cacgccaata aactgaatat gcgttgataa
     5821 tagtgcacga ctgccggatg cggcgtgaac gccttatccg cctacatttc gcccgcaggc
     5881 cgtaggcatg ataagacgcg catagcgtcg catcaggcat ctgcgcacga cttatctatg
     5941 tttaccacat caaatcatca ggcactttga agtcggcata tgggtcgtct tcatcctgct
     6001 cttcggcact gagcgcgctg tgcaacacaa tactgctggc atcacgctga gcaattttat
     6061 cggcaacgct ggcgggaata atggcgtatt caccttcgct attgttatcg accaacaagc
     6121 gggcaatcgc cagacgacca ttaatcaact gcgcctgtgt gagcttatcg acaaaaatct
     6181 tcttaatcag attgccgtca gtgaagttaa aaccaatatc accattggca atggtgattc
     6241 ggttcatttc aataagctgc ttcacctggg ctttatattc tttcgccaac gccgcttgtt
     6301 tttgctgttc gctaagctgt ttatcacgct caagctgtgc ctttttattt tcttctaccg
     6361 ccgcccgagc ttcacgcgcc tgaacgcgcg attttttcgc cgttctctcg accttcgccg
     6421 cttttttgct ggtgactaat cctgctttga gcaactgctc ttgtaaggta agttttgcca
     6481 tctttgtttc caaacccgct gaataatttt tagcgagtat acctgtaaac gccgtgggtg
     6541 tattgggggc aagtcccttt cgcccctgct aaataaggcg cgtcaggcat cggcacacgg
     6601 ctgcattctt gatctccgac tcatcaaaat aaacaccgcc aacccagcaa caagaatacc
     6661 cggtgcagaa gcggccatta cgccgaccgt gccagtgcct aatgccagca ttttcccggc
     6721 cagtaacgga ccgctcatag cccccagacg ccctaccgcc acggctgttc ccacacctgt
     6781 tgcgcggatc tgcgaactgt aaaacaacgg tgccagggca tacaaaacgc tttgcccacc
     6841 tgtcgcaaac aaccccgcga caaatcccgc cagcaacata ccgttaaatg acgacaccgt
     6901 tccaagcgcc agcagcgaag ctaacatgcc gctataaatc agtagcgaca tggttactgg
     6961 acgcagctta tccatcaatg cgcccaacat taacgtcccg cttgccgccc ccatttgcag
     7021 ggcaaacatc acccctgccg cctgcgatgg ctggaatcct tgctccacca aaagtagcgg
     7081 tagccagttg atcaacatat aaaccaccag cagagtgaag aaataacaca accacagcag
     7141 cagcgtcgcg gttgccgttt ctggcgcaaa taaggcacgc agtggtggcg cagcctgttt
     7201 ttcgccagcg aaaaccgctg actccggcag ccagcgcatt aatagcggca ccagaatcaa
     7261 cggcaccaca ccacctaccc aaaacaccgt ttgccatgct aagtttgccc ccgcgaaacc
     7321 cagtgtcgcc gccagcgccg cgccaatggg aacaccgcaa tacatcaggc tcactgccgt
     7381 cccacgaaaa cgtggacccg cggcttcaga cgtcagggcg ataagattcg gcaacgccgc
     7441 ccccagcccg acaccggtca tcagccgcgc aaagaccagt gaggggaaat cccaggcaat
     7501 cgccgttgcc agtgagaaca aaccaaacag cgcaactgag ccaatcaaaa tactcttacg
     7561 accataacgg tctgccagca tcccgccaac caacgcgccg ggtagcaaac cgagtattcc
     7621 ggcgctaaat atccagccca tttgcatttt atcgagtgcg aaagcctggg cgatgccacc
     7681 cgccgcaatg ccagccgcct gaagatccag cccttccatc agagcgacca aaaaacaaag
     7741 cccgatggtc agcatcaggc gggatgaaga tgatgaaggg gtacgagtcg acataagatg
     7801 aacctcgttg cgagtgcagg tgaatcgcga ctgatacatt gccagccgcg taatcagaac
     7861 tatttaatga ctgcctacgg gcgaaatgta ggccggacaa gacgtttatc gcatccggca
     7921 gtcgtgtgca gatgcctgat gcgacgctaa cgcgtcttat caggcctaca aattctcacg
     7981 ccacccgtag gccggataag gtgtttacgc cgcatccggc agtcgtgctg ggatgggcat
     8041 acaaaatgca cgttctcggc agtcatcatt atttgttgtt gcgcagatcc agcgccacgt
     8101 caacgatcat atcctcctgg ccgccaacca tccggcgttt gcccagctca acgagaatat
     8161 ccaccgcact taagccataa cgcgccgccg ccgtttcaca gtgacgcagg aagctcgagt
     8221 aaacaccagc gtatcccagc gccagcgttt cgcgatcgac tcgtaccggt cgatcctgca
     8281 acggacgcac caggtcgtca gcggcatcca ttaacgcata gagatcggtc ccatgctgcc
     8341 agcccagttt atccgcggcg gcaataaaca cttccagcgg tgcgttacct gcgcccgcgc
     8401 ccattcccgc gaggctggcg tcgattcggt cgcagccctc ttccaccgcc gcgatagagt
     8461 tcgccacgcc aagactcagg ttatggtgag cgtgcatgcc agtttgcgtt tctggtttca
     8521 gctctgcttt cagggcgcgg aaacggtcac ggatatcgct catgttcatc gcaccgccag
     8581 aatccaccac ataaatacag gtcgcaccgt agccttccat cagctttgcc tgctttgcga
     8641 gattctccgg cgtggtcata tggctcatca tcagaaaacc aacggtgtcc attccgagct
     8701 cgcgggcata ctgaatatgc tgggcggaaa catcagcttc ggtacagtgc gttgccacac
     8761 gaaccacccg cgcgccagcc tgccaggcat ttttcagatc gtgaatagtg ccgattcctg
     8821 gcagcaacaa cgtcgcgatt ttggcgtgct tcaccacatc cgccgctgct tcaatccatt
     8881 caaggtcgct atgtgcgccg aaaccatagt taaagctgga accctgcaaa ccgtcgccat
     8941 gggccacttc aatcgaatcc acgcgggcat cgtccagtgc tttggcaatc tggcgaacgt
     9001 tttccagcga atactgatga cgaatggcgt gcataccgtc acgcaacgtg acgtccgaga
     9061 tataaagttt tttaccgttc atgccgcttc tcctgccttg cgcgccagtg actgggccat
     9121 tttttccgct gtcgccagcg cactggaagt cataatgtcg aggttgcccg cataggcagg
     9181 cagataatgc gctgcgcctt cgacttccag ccagaccgct gttttcagtc cggagaattg
     9241 ccccacgccc ggtaaattga ccggtttatc ctgcgggata acttcaaact gcacgcgctg
     9301 tttcaggcga taacccggta cgtaagcctg caccgcctca gccatttcat tgattgaggc
     9361 ttcgatatca tcttgtgaag cttcgtcgct caatacatac accgtgtcac gcatcatcaa
     9421 cggtggctct gctgggttaa gcacaataat cgccttccct tttgccgcgc cgcccaccac
     9481 ttcaatggct cgggaagtgg tttccgtaaa ttcatcgata ttggcacgcg tgccaggtcc
     9541 ggcagattta ctggcgatag aagcgataat ttcggcgtaa tgaacacgcg ccacgcgtga
     9601 aactgccgcc accattggaa tggtggcctg gccgccgcag gtgaccatgt tgacgttcag
     9661 ttgatcgacg ttcgcctcga ggttaaccac cggcacgcag taagggccga tggcagcagg
     9721 cgtcaggtca attaagcgaa tatccggttt cgcttcgcgt aaagcggcat cgtttttcac
     9781 atgcgcgccc gcgctggtcg catcaaatac aatgtcgata tcagcaaatt caggcatgtt
     9841 catcagcccg atcacccctt catgggtggt ggcgacgccc atacgtctgg cacgcgccag
     9901 accgtcggac tgaggatcaa tgccgaccat caccgccatc tccagatgct gaccgtgacg
     9961 caaaatttta atcatcagat cggtgccaat gttgccagaa ccgataatgg cgactttacg
    10021 cttactcatg acagacttcc ttttggggct gcgcttgaaa atgtcgcagc aactgaacct
    10081 atgccttcaa tatgggcttc aaaacgatcg cccgcattca ctgccaccat cggacctaat
    10141 gccccggtaa gaatgatatc tccggcgcgc agcggttcac ccaggctggc cattttgcgt
    10201 gccagccaga cggcggcatt aagcggatgt cccaggcatt cgctgccgcg cccgctggaa
    10261 acctcttcgt tattacgcgt catcttcatg gcgcagtttt tcaggtctaa ccccgccgga
    10321 cgttgcgccg gaccgccgat gacatacacc ccgcaggaag cgttatctgc cacggtatcg
    10381 acaaactgaa tcgaccagtc gcgaatgcgg ctacccacca cctccagcgc cggaagtacc
    10441 cattcaatgg cgttatacag ttcgtcgaag gtgatatcgg ttgcgggcaa atcgcggttc
    10501 aacaccagtg cgatctccgc ttcaatgcgg gcttgcagaa cacgggaaaa aggaatgatt
    10561 tcgttatcgc cataacacat gtcggcaaat aatgtcccaa aatccggttg atcaacgccc
    10621 agttgttgct gcactttcgg atgcgtcagg cccactttac gccctaccac gcgacgcccc
    10681 tgcgcaacgt catattgcac atttatgtgc tgaatggcgt aggccgcttc agcgttatcg
    10741 ataccaatca gatcgcgcag cggcgcaatc gcttcgccct gctctgcggc gcggcgtaaa
    10801 tccgccgcca gttgctcaag agtatgcttc gtcatgacca ttccttaagg gcgtgcgagg
    10861 aaattcagca ccaattgatt gaaagcgtcg gcatgttccc actgcgccca gtgaccacag
    10921 tcgcggaaga tatgcagttc agaaccggca atgccggaca gcagacgcag acccgcatcc
    10981 atcggcacaa agcggtcgtt gcgcccccag acaatcaggg tttgcgcttt gatttccgcc
    11041 agacgtgggc caaaatccgg gaactgtttc ggattagctt ccaggctctt aacgaagttt
    11101 tccaggtgat cgcggcgcga cagcatatta ttcaggcgcg cttcaaacag ggcgtcggtc
    11161 aaatcgctgg tatcgaaaac gaagatatcc atcatcagct tcaggttttc gatagtcggc
    11221 tgacgataaa gctgattcag tcgcttaata ccttcggttg gcatcggcgt aaacaaactc
    11281 atgccgcccg taccgccgcc catcagcacc agtttgccga cgcgctccgg ccagttaagg
    11341 gtgaacgcca tagaactatg gccgcccatc gagttgccca gcaggtggat tttggcgata
    11401 tccagttgat ccaccacgct tttcaggatt cgtgcattaa gatccgatcg tgaaccacta
    11461 ttaacgatcg aatcgctttt gccccaaccc ggacaatcca gcaggattac ccgatagccc
    11521 gcctctacca gcggatcgat attgcggctg aagttcgccc agccagtagc acccgggccg
    11581 gaaccgtgca gcaggacaac ggtttcgtcg ccttgtccgc agtcattaaa atggatgcgc
    11641 agcgttttac ccgcttcttc tacattcaga aaacggctgg tggcggcttc ggtttgtggc
    11701 tgataactca tcatcttctc ctgcatattc agttctctgt tctggcgctt aacgagccaa
    11761 atccggcaat ccactccggg attgggcggt aataacgccc ttcgctacgc cagttgccaa
    11821 atgcagaaat agcggcaaaa gcggcgaccc aggttttgat ttcatgtgtc gactttccgg
    11881 caatggcgga aagctcttcg ttactgacgg catccagttc ctgtatgcgt ccctgctcca
    11941 gcaaagtcat gaactggtta tcccaaatcg ggttgagcgg atgcagagtt ctctgatcct
    12001 caacaaactt cacagcggcg ctaatcaccc gttgctgacg caattcgcgc tcactggcgg
    12061 gtaaatcttt cccgctcccc aacagacggt cgcgcatatg ggcatcggct ttcgccagtt
    12121 cgggcaccgg cggctgatgg gaaagcccac cggaacccag gaacagcacg cgtttattga
    12181 gagtgctggt gaaacgtcca atggcttcac ccaacatgcg ggtacgctgg aaaccgggca
    12241 gcggcgtggc gacaccgttg atgaacacag gcagaactgg caccttatcc agcccaccga
    12301 gcaggaactc cagcggctgg gagaacccgt ggtccacctg catacagtaa gaaacggcaa
    12361 gatcgatccc gctcttcatg acggcatgcg cacaggcctc cgccagctcc acaggcacgg
    12421 gcagctctcc tgccgcactg ccgaaatcac caattgccgt cgctccaacg cctaaacaga
    12481 acggtggcat cacgtcatag aaaaagccgt tgtagtgatc tggcgcaaac agcaccacca
    12541 gttcagggga gaaagccgca atacgctcgc gggcgctggc aatcacgcca ttgacctcat
    12601 cgagtacctc ttgtgccggg tcgacatacc ccaccagcgg cgagtgggaa agacaatgaa
    12661 gataagcgtg catatcaggc taccttttcg acagaaacgt cggcatcagg gcgggtcagc
    12721 gtcatcaccg acgccagttt attcagggtc ttgcccaggg tttgcggaat ggcggtggcg
    12781 gcaacaaagc gatccgggcg catcaccacc agcgaagcat tgtgttgtgc gaaccagcta
    12841 cgcagacgac cttgcgtatc gcccacgcgt agcacgccgt cgtggttatc ctgtgccgta
    12901 tgaatttgca cttccggcac cacctgaata aagcgtgtgc ccaacgcgcg ccactgctgg
    12961 atttgctcgt cgctcatccc ccacagtgga ttgcatcccc agccaattac cgcgaagttc
    13021 gcgccgatcg cgttatcgag cagcgtcacg tcgccgtttt ccagcgtgac tttcggctga
    13081 ataaacatct tgccgaccgg agagtgcttc gcttcgccct cacgtaccag cgcaccgccg
    13141 taatattgcg gcatcggctt gaagcgcatt tcgaggaagt agcgttttac tggcggcaga
    13201 taattcagca gccaggaaac gccgtcacgt aacgtaccct gccagcgttt cggcggagcc
    13261 agcacgttgc ccgccgtcac ggacaggtca atcatcgctt tggcgtgatc gcgacgttct
    13321 tgttgatagg tattgagcag cgcatcgcgg gctttcccct ggataaccaa cgccagtttc
    13381 caggcgaggt taaaggcgtc gcgcatacca ctgttatagc cctgcccctg ccacaccggc
    13441 atgatgtgcg cggcatcgcc cgccagcagt acgcgatcaa tacggaaacg ttgcgccagt
    13501 cgcgcgttat gggtgtagac acgctggcga atcaattcaa cattgtccgg attaggcagc
    13561 actttgctta acagcttgcg catattttgc ggctcacgca gctgctcttc ggtttctccc
    13621 ggcatcacca taaattcaaa gcgacgtacc gcatgaggca gcgcggcaga aacatacggg
    13681 cgcaccggat cgcaacacaa atagatatgc ggcgtactta acggatcgtt ggcgatatct
    13741 accacaatcc actgatttgg cgcagtttta ccttcaaacg gcacattcag ggtgcgacga
    13801 acaaaacttg ccccaccatc acaggccacc agccactggg ctttgatcgt ttcccgctgc
    13861 ccttctgccg ttttcaggtg caaggtcact tcgtcatctt gctggctgaa ggcctccagc
    13921 tcgcgggaaa acaagcagcg cacattcgga aaacgcgaca acccttccag catcaccgca
    13981 tcgacctgtg gctgaataaa ggcgttacgg cgcggccagc caaattcatc ggtcattggc
    14041 tgaatatcag caaaacagcg gccttttggg gtgagaaaac gcatcgcgtg ccacggtgta
    14101 gtgtgcggca gaacattctc gaccaggccg accgactgca tggtgcgcag cgcctcgtca
    14161 tcaataccaa tcgcacgcgg gtagtcgatc aacttatcga gtttctccac caccagcacg
    14221 tcaatgccca tctgaccgag atagttcgcc atcatcagcc cgaccggacc ggcaccagag
    14281 atcgccacct gaacgctatg gttaacagca ggctggatgt cagggtgttg tattgccatt
    14341 tcagtacctc acgactcgga caaaatgccg ttgcgcgcac agtacggcgc aacttatttt
    14401 gttaaaaaca tgtaaatgat tttttattgt gcgctcagta taggaagggt gttttcggct
    14461 acaatcaaaa catgcccgaa tgtgcaccag gtgcacaacg ttgttttaac tatagaaatg
    14521 tcaattaata tgcagaacaa tgagcagacg gaatacaaaa ccgtgcgcgg cttaacccgc
    14581 ggtctaatgt tattaaatat gttaaataaa cttgatggcg gtgccagcgt cgggctgctg
    14641 gcggaactca gcggcctgca tcgcaccact gtgcggcgac tgctggagac gctgcaggaa
    14701 gagggatatg tccgccgtag cccctccgat gacagttttc gtctgaccat caaagtgcgg
    14761 caattaagcg aaggatttcg tgacgaacag tggatttctg cactggcggc cccgctgctg
    14821 ggcgatctgt tgcgcgaagt ggtatggcca acagatgtgt ccaccctgga tgttgatgca
    14881 atggtggtac gcgaaaccac ccaccgtttc agccgcttat cctttcaccg ggcaatggtc
    14941 gggcgacgtt taccgctcct gaaaaccgcc tcgggcctga cctggctggc cttttgcccg
    15001 gaacaagaac gcaaggaatt aatcgaaatg ttagccgccc gccccggtga tgactatcaa
    15061 ctggcacgag aaccgttaaa gctgcaagcc attctggcgc gcgcgcgcaa agagggttac
    15121 ggacagaact accgcggctg ggatcaggag gagaagatcg cctctatcgc cgtaccgctg
    15181 cgcagtgaac aacgggtgat tggctgtctg aatctggtgt atatggcgag cgcaatgacc
    15241 attgaacagg ccgcggaaaa gcatcttccg gcgctacaac gggtagcaaa acagatcgaa
    15301 gaaggggttg aatcgcaggc tattctggtg gccggacggc gaagcggcgt gcatttacat
    15361 tgacaccatc gaatggcgca aaacctttcg cggtatggca tgatagcgcc cggaagagag
    15421 tcaattcagg gtggtgaatg tgaaaccagt aacgctatac gatgtcgcag agtatgccgg
    15481 agtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac
    15541 gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca
    15601 acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca
    15661 cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt
    15721 ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct
    15781 tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat
    15841 tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac
    15901 acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct
    15961 ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc
    16021 gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc
    16081 ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa
    16141 tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat
    16201 gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga
    16261 cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg
    16321 cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcaggtcc aggcggtgaa
    16381 gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac
    16441 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaagtttc
    16501 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagtcagctc actcattagg
    16561 caccccaggc tttacacttt atgcgtccgg ctcgtatgtt gtgtgaaatt gtgagcgaat
    16621 aacaatttca cacaggaaac agctatgacc atgattacgg attcactggc cgtcgtatta
    16681 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
    16741 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
    16801 cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggttccggaa
    16861 agctggctgg agtgcgatct tcctgtcgcc gatactgtcg tcgtcccctc aaactggcag
    16921 atgcacggtt acgacgcgcc catctacacc aacgtgacct atcccattac ggtcaatccg
    16981 ccatttgttc ccacggagaa tccgactggt tgttactcgc tcacatttaa tgttgatgaa
    17041 agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttaactc ggcgtttcat
    17101 ctgtggtgca acgggcgctg ggtcggttac ggccaggaca gccgtttgcc gtctgaattt
    17161 gacctgagca catttttacg cgccggagaa aaccgcctcg cggtgatggt gctgcgctgg
    17221 agtgacggca gttatctgga agatcaggat atgtggcgga tgagcggcat tttccgtgac
    17281 gtctcgttgc tgcacaaacc gagcacacaa atcagcgatt tccatgttgc cactcacttt
    17341 aatgatgatt tcagccgcgc ggtactggaa gcagaagttc agatgtatgg cgagctgcgc
    17401 gatgagctgc gggtgacggt ttctttgtgg cagggtgaaa cgcaggtcgc cagcggcacc
    17461 gcgcctttcg gcggtgaaat tatcgatgag cgtggtggtt atgccgatcg cgtcacacta
    17521 cgtctgaacg tcgaaaaccc gaaactgtgg agcgccgaaa tcccgaatct ctatcgtgcg
    17581 gtggttgaac tgcacaccgc cgacggcacg ctgattgaag cagaagccag cgatgtcggt
    17641 ttccgcgagg tgcggattga aaatggtctg ctgctgctga acggcaagcc gttgctgatt
    17701 cgcggcgtta accgtcacga gcatcatcct ctgcatggtc aggtcatgga tgagcagacg
    17761 atggtgcagg atatcctgct gatgaagcag aacaacttta acgccgtgcg ctgttcgcat
    17821 tatccgaacc atccgctgtg gtacacgctg tgcgaccgct acggcctgta tgtggtagat
    17881 gaagccaaca ttgaaaccca cggcatggtg ccaatgaatc gtctgaccga tgatccgcgc
    17941 tggctaccgg cgatgagcga acgcgtaacg cgaatggtgc agcgcgatcg taatcacccg
    18001 agtgtgatca tctggtcgct gggaaatgag tcaggccacg gcgctaatca cgacgcgctg
    18061 tatcgctgga tcaaatctgt cgatccatcc cgcccggtgc agtatgaagg cggcggagcc
    18121 gacaccacgg ccaccgatat tatttgcccg atgtacgcgc gcgtggatga agaccagccc
    18181 ttcccggctg tgccgaaatg gtccatcaaa aaatggcttt cgctacctgg agagacgcgc
    18241 ccgctgatcc tttgcgaata cgcccacgcg atgggtaaca gtcttggcgg cttcgctaaa
    18301 tactggcagg cgtttcgtca gtacccccgt ttacagggcg gcttcgtctg ggactgggtg
    18361 gatcagtcgc tgattaaata tgatgaaaat ggcaatccgt ggtcggctta cggcggtgat
    18421 tttggcgata cgccgaacga tcgccagttc tgcatgaacg gtctggtctt tgccgaccgc
    18481 acgccgcatc cagcgctgac ggaagcaaaa caccagcaac agtttttcca gttccgttta
    18541 tccgggcaaa ccatcgaagt gaccagcgaa tacctgttcc gtcatagcga taacgagctc
    18601 ctgcactgga tggtggcgct ggatggcaag ccgctggcaa gcggtgaagt gcctctggat
    18661 gtcgctccac aaggtaaaca gttgattgaa ctgcctgaac taccgcagcc ggagagcgcc
    18721 gggcaactct ggctcacagt acgcgtagtg caaccgaacg cgaccgcatg gtcagaagcc
    18781 gggcacatca gcgcctggca gcagtggcgt ctggcggaaa acctcagtgt gacgctcccc
    18841 tccgcgtccc atatcatccc gcaactaacc accagcgaaa cggatttttg catcgagctg
    18901 ggtaataagc gttggcaatt taaccgtcag tcaggccttc tttcacagat gtggattggc
    18961 gatgaaaaac aactgctgac gccgctgcgc gatcagttca cccgcgcgcc gctggataac
    19021 gacattggcg taagtgaagc gacccgcatt gaccctaacg cctgggtcga acgctggaag
    19081 gcggcgggcc attaccaggc cgaagcagcg ttgttgcagt gcacggcaga tacacttgct
    19141 gatgcggtgc tgattacgac cgctcacgcg tggcagcatc aggggaaaac cttatttatc
    19201 agccggaaaa cctaccggat tgatggtagt ggtcaaatgg cgattaccgt tgatgttgaa
    19261 gtggcgagcg atacaccgca tccggcgcgg attggcctga actgccagct ggcgcaggta
    19321 gcagagcggg taaactggct cggattaggg ccgcaagaaa actatcccga ccgccttact
    19381 gccgcctgtt ttgaccgctg ggatctgcca ttgtcagaca tgtatacccc gtacgtcttc
    19441 ccgagcgaaa acggtctgcg ctgcgggacg cgcgaattga attatggccc acaccagtgg
    19501 cgcggcgact tccagttcaa catcagccgc tacagtcaac agcaactgat ggaaaccagc
    19561 catcgccatc tgctgcacgc agaagaaggc acatggctga atatcgacgg tttccatatg
    19621 gggattggtg gcgacgactc ctggagcccg tcagtgtcgg cggaattcca gctgagcgcc
    19681 ggtcgctacc attaccagtt ggtctggtgt caaaaataat aataaccggg caggccatgt
    19741 ctgcccgtat ttcgcgtaag gaaatccatt atgtactatt taaaaaacac aaacttttgg
    19801 atgttcggtt tattcttttt cttttacttt tttatcatgg gagcctactt cccgtttttc
    19861 ccgatttggc tacatgacat caaccatatc agcaaaagtg atacgggtat tatttttgcc
    19921 gctatttctc tgttctcgct attattccaa ccgctgtttg gtctgctttc tgacaaactc
    19981 gggctgcgca aatacctgct gtggattatt accggcatgt tagtgatgtt tgcgccgttc
    20041 tttattttta tcttcgggcc actgttacaa tacaacattt tagtaggatc gattgttggt
    20101 ggtatttatc taggcttttg ttttaacgcc ggtgcgccag cagtagaggc atttattgag
    20161 aaagtcagcc gtcgcagtaa tttcgaattt ggtcgcgcgc ggatgtttgg ctgtgttggc
    20221 tgggcgctgt gtgcctcgat tgtcggcatc atgttcacca tcaataatca gtttgttttc
    20281 tggctgggct ctggctgtgc actcatcctc gccgttttac tctttttcgc caaaacggat
    20341 gcgccctctt ctgccacggt tgccaatgcg gtaggtgcca accattcggc atttagcctt
    20401 aagctggcac tggaactgtt cagacagcca aaactgtggt ttttgtcact gtatgttatt
    20461 ggcgtttcct gcacctacga tgtttttgac caacagtttg ctaatttctt tacttcgttc
    20521 tttgctaccg gtgaacaggg tacgcgggta tttggctacg taacgacaat gggcgaatta
    20581 cttaacgcct cgattatgtt ctttgcgcca ctgatcatta atcgcatcgg tgggaaaaac
    20641 gccctgctgc tggctggcac tattatgtct gtacgtatta ttggctcatc gttcgccacc
    20701 tcagcgctgg aagtggttat tctgaaaacg ctgcatatgt ttgaagtacc gttcctgctg
    20761 gtgggctgct ttaaatatat taccagccag tttgaagtgc gtttttcagc gacgatttat
    20821 ctggtctgtt tctgcttctt taagcaactg gcgatgattt ttatgtctgt actggcgggc
    20881 aatatgtatg aaagcatcgg tttccagggc gcttatctgg tgctgggtct ggtggcgctg
    20941 ggcttcacct taatttccgt gttcacgctt agcggccccg gcccgctttc cctgctgcgt
    21001 cgtcaggtga atgaagtcgc ttaagcaatc aatgtcggat gcggcgcgag cgccttatcc
    21061 gaccaacata tcataacgga gtgatcgcat tgaacatgcc aatgaccgaa agaataagag
    21121 caggcaagct atttaccgat atgtgcgaag gcttaccgga aaaaagactt cgtgggaaaa
    21181 cgttaatgta tgagtttaat cactcgcatc catcagaagt tgaaaaaaga gaaagcctga
    21241 ttaaagaaat gtttgccacg gtaggggaaa acgcctgggt agaaccgcct gtctatttct
    21301 cttacggttc caacatccat ataggccgca atttttatgc aaatttcaat ttaaccattg
    21361 tcgatgacta cacggtaaca atcggtgata acgtactgat tgcacccaac gttactcttt
    21421 ccgttacggg acaccctgta caccatgaat tgagaaaaaa cggcgagatg tactcttttc
    21481 cgataacgat tggcaataac gtctggatcg gaagtcatgt ggttattaat ccaggcgtca
    21541 ccatcgggga taattctgtt attggcgcgg gtagtatcgt cacaaaagac attccaccaa
    21601 acgtcgtggc ggctggcgtt ccttgtcggg ttattcgcga aataaacgac cgggata
//
LOCUS       DAZGVQ010000084        21384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000084 DAZGVQ010000000
VERSION     DAZGVQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.085"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..547)
                     /locus_tag="ACHLAG_003027"
     CDS             complement(<1..547)
                     /locus_tag="ACHLAG_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP0656213.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYN"
     gene            complement(745..1218)
                     /gene="sbmC"
                     /locus_tag="ACHLAG_003028"
     CDS             complement(745..1218)
                     /gene="sbmC"
                     /locus_tag="ACHLAG_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFP0656214.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEITGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(1337..2503)
                     /gene="dacD"
                     /locus_tag="ACHLAG_003029"
     CDS             complement(1337..2503)
                     /gene="dacD"
                     /locus_tag="ACHLAG_003029"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFP0656215.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            2712..4139
                     /gene="sbcB"
                     /locus_tag="ACHLAG_003030"
     CDS             2712..4139
                     /gene="sbcB"
                     /locus_tag="ACHLAG_003030"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFP0656216.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(4182..4409)
                     /gene="tsuB"
                     /locus_tag="ACHLAG_003031"
     CDS             complement(4182..4409)
                     /gene="tsuB"
                     /locus_tag="ACHLAG_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFP0656217.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(4423..5481)
                     /gene="tsuA"
                     /locus_tag="ACHLAG_003032"
     CDS             complement(4423..5481)
                     /gene="tsuA"
                     /locus_tag="ACHLAG_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFP0656218.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIVKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(5660..7018)
                     /gene="plaP"
                     /locus_tag="ACHLAG_003033"
     CDS             complement(5660..7018)
                     /gene="plaP"
                     /locus_tag="ACHLAG_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFP0656219.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWLNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(7008..7070)
                     /gene="yoeI"
                     /locus_tag="ACHLAG_003034"
     CDS             complement(7008..7070)
                     /gene="yoeI"
                     /locus_tag="ACHLAG_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFP0656220.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(7285..8214)
                     /gene="yeeY"
                     /locus_tag="ACHLAG_003035"
     CDS             complement(7285..8214)
                     /gene="yeeY"
                     /locus_tag="ACHLAG_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0656221.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(8260..9084)
                     /gene="yeeZ"
                     /locus_tag="ACHLAG_003036"
     CDS             complement(8260..9084)
                     /gene="yeeZ"
                     /locus_tag="ACHLAG_003036"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0656222.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(9167..9421)
                     /gene="yoeB"
                     /locus_tag="ACHLAG_003037"
     CDS             complement(9167..9421)
                     /gene="yoeB"
                     /locus_tag="ACHLAG_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFP0656223.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(9418..9669)
                     /gene="yefM"
                     /locus_tag="ACHLAG_003038"
     CDS             complement(9418..9669)
                     /gene="yefM"
                     /locus_tag="ACHLAG_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFP0656224.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            9952..10002
                     /gene="hisL"
                     /locus_tag="ACHLAG_003039"
     CDS             9952..10002
                     /gene="hisL"
                     /locus_tag="ACHLAG_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFP0656225.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    9978..10101
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            10148..11047
                     /gene="hisG"
                     /locus_tag="ACHLAG_003040"
     CDS             10148..11047
                     /gene="hisG"
                     /locus_tag="ACHLAG_003040"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFP0656226.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            11053..12357
                     /gene="hisD"
                     /locus_tag="ACHLAG_003041"
     CDS             11053..12357
                     /gene="hisD"
                     /locus_tag="ACHLAG_003041"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFP0656227.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALSASRQIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            12354..13424
                     /gene="hisC"
                     /locus_tag="ACHLAG_003042"
     CDS             12354..13424
                     /gene="hisC"
                     /locus_tag="ACHLAG_003042"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFP0656228.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPFLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            13424..14491
                     /gene="hisB"
                     /locus_tag="ACHLAG_003043"
     CDS             13424..14491
                     /gene="hisB"
                     /locus_tag="ACHLAG_003043"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFP0656229.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELMKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGITGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            14491..15081
                     /gene="hisH"
                     /locus_tag="ACHLAG_003044"
     CDS             14491..15081
                     /gene="hisH"
                     /locus_tag="ACHLAG_003044"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFP0656230.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVHKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            15081..15818
                     /gene="hisA"
                     /locus_tag="ACHLAG_003045"
     CDS             15081..15818
                     /gene="hisA"
                     /locus_tag="ACHLAG_003045"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFP0656231.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDIDDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            15800..16576
                     /gene="hisF"
                     /locus_tag="ACHLAG_003046"
     CDS             15800..16576
                     /gene="hisF"
                     /locus_tag="ACHLAG_003046"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFP0656232.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLDDAAKILSFGADKISI
                     NSPALADPTLITHLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            16570..17181
                     /gene="hisIE"
                     /locus_tag="ACHLAG_003047"
     CDS             16570..17181
                     /gene="hisIE"
                     /locus_tag="ACHLAG_003047"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFP0656233.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(17279..18256)
                     /gene="wzzB"
                     /locus_tag="ACHLAG_003048"
     CDS             complement(17279..18256)
                     /gene="wzzB"
                     /locus_tag="ACHLAG_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFP0656234.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYGSAAPKVSEIQSMLIGRFSTT
                     FSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAQYIQQVDDQVN
                     EELEQDLKDNIALQMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(18403..19569)
                     /gene="ugd"
                     /locus_tag="ACHLAG_003049"
     CDS             complement(18403..19569)
                     /gene="ugd"
                     /locus_tag="ACHLAG_003049"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFP0656235.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(19818..21224)
                     /gene="gndA"
                     /locus_tag="ACHLAG_003050"
     CDS             complement(19818..21224)
                     /gene="gndA"
                     /locus_tag="ACHLAG_003050"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFP0656236.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDSEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQLAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT         5510 a         5537 c         5250 g         5087 t
ORIGIN      
        1 gattatactc ggtcagactt ttcgcaagtt gaatgcgcca gtggatgaac gcccgttgtg
       61 gccagatacc ggtaaacaac attgccagta aagagccgag gatcacatcg ccgcttcgcc
      121 ataacgccgt atcaatttca cctgtcgggg aaccaacaac aattgccagc gtcaccccaa
      181 tcaataaacc ttgatacggt ttcttgccca gcgccagcca accgcaaaga aacatcgccg
      241 ccgcgcacca gactaacatc agcggtaacg aaattaactc cagttgcaga gcgataagac
      301 ctaaaatcga acccaacacc gtaccgccaa tacgttcgaa ggcgcgaggg acaacgttac
      361 cccagaacga gattggcccc ataatcacca ccatggtgac cagcggccag gtgctttccg
      421 ggatagtaaa caggcggata atgagaaaag tgagcaggaa tgccagcgcg acccgagtac
      481 catgcacaat gcggtaatgg cggtataccc ggatttcaaa cgggcttaat gacttatcgg
      541 cacgcacgca gaaactccgt aatgaagatt aaaagcgctc ataactcatc tgtcggccag
      601 aaggcgcacc tctgatttat ccgacggaca taccgggaac agtgtaattt cccggcagga
      661 gcgaaaaatt tgaaaggcgc atcttactct tttcgctttc aaaaaaagag tggtcttcgc
      721 gtcaacacac cgccctgaga tgaattagtg atgttttggc tgcaccgcaa catacatttc
      781 gatatcccag tacccatctt ccgcgccatt gttcaagtaa acctcgaagc agggctttgg
      841 taacatttca taagcactgt cctgcaagag actattaaag aactgatacc agggtttagc
      901 aaaatcatca ccgactacac gagctaccgc caccgcatac tgaccacctg taatttctgt
      961 cagaatgacg ccctcactgt tttcgggaag cgtaaagtaa cccggcaccg tcacgacggt
     1021 gtcgcagcgt aatttttcgg cgggtgtttc atctggattg tcgtaataga cagcaaccca
     1081 ctccttcggc acaatatttt tgttatctac ccacatcatc aactgctcaa agcctttctt
     1141 taccgtctgt tcccacgggc caacgagatg gaaacctgca acggtacgtt tctcttcctg
     1201 cttaatctcg tagttcatga cgcctccatt aacactgttt ttatatatag tatagttgca
     1261 atattaaaac cacaaggaat gagtgttgat tatgcgagca gactcgcact cctgccagtc
     1321 tgctgcaaaa gaaaggtcag gccttatggt ggaaataatc actcaggcgg gaaaacatgc
     1381 cgccttcccc gacagattcc agggtaacca gcggccagtg cgccacctgt ttatcacggt
     1441 cgtaaagttc aatttcccct acccgctgat gggcgctaat tggcgcggtg agctctttac
     1501 catcaagggt atatttggct ttgatatgtg gaatttcggc tttcggtagc accatccaga
     1561 actcttgttc cgttcccagg tcgatatttt ctttatcgcc ataccagatg cgttccgttc
     1621 cgaccttttt cccacggtgc aaaatttgca ccgtagtaaa gttttgttgc ccccaacgca
     1681 gtaattttct tgcctcttcc tcacgacctt ttgcactgtc agcccccatt accactgcaa
     1741 tgagacgacg ctgcccatct acagccgaag caatgagatt aaacccggca ccagaagtat
     1801 gacccgtttt caggccgtca acattcatgg ttttatccca caacaacccg ttacggtttt
     1861 gctgggtgat accgttccag gtgagacttt tctcactgta catatgataa aactcgggct
     1921 cgccgtggat gatagcgcga gaaagcacag ctaaatcata agccgagcta tgctggccag
     1981 gtgcatccag accatgcact gtttcaaaat gcgtatcctt gagatgcagc ttctcggcat
     2041 agttgttcat catttcaaca aactgccgtt gcccaccggc aatatagtca gccagagcaa
     2101 cacaagcgtc atttccggaa tccacaatta aaccacggct taaatcacgt accgatacgc
     2161 gatcgccctc tttcaaaaac atcagtgaag aaccgacaaa caccggatta tctttcgccc
     2221 acgcatcgcg ccccacggtg acaatatcgt ctggcgtaat gcgatgacta tcgatagcgc
     2281 gatccacgac ataacccgtc atcagctttg tcaggctggc gggattgcgc tgttgatgct
     2341 cattacccgc ggtgaggatc tgaccggtgg tgtaatccat caatacccag gacccggcat
     2401 gaatctctgg aggctgaggt gaaaaaggaa tgttttccgc cgcaaaacca gacgataagt
     2461 taaaaacgaa caaagaagca gcaataataa gacggcgttt caacagcaaa ccctcaggag
     2521 tttcaaatag ctgttctttt tacggaaata cttatgaact ggctggaata aagtgcaaga
     2581 aaatgtgact accctctcat ttttatctga catgatctgt tgccactcgc tgccaaattg
     2641 tggcgctaaa gctgattagc acggtgatat ttgatactct ggcagacagc agaaataacg
     2701 gatttaacct aatgatgaat gacggtaagc aacaatctac ctttttgttt cacgattacg
     2761 aaacctttgg cacgcacccc gcgttagatc gccctgcaca gttcgcagcc attcgcaccg
     2821 atagcgaatt caatgtcatc ggcgaacccg aagtctttta ctgcaagccc gcggatgact
     2881 atttacccca gcctggagca gtattaatta ccggtattac cccgcaggaa gcacgggcga
     2941 aaggagaaaa cgaagccgcg tttgccgccc gtattcactc gctttttacc gtaccgaaga
     3001 cctgtattct gggctacaac aatgtgcgtt tcgacgacga agtcacacgc aacgtttttt
     3061 atcgtaattt ctacgatcct tacgcctgga gctggcagca tgataactcg cgctgggatt
     3121 tgttggatgt tatgcgtgcc tgttatgccc tgcgcccgga aggaataaac tggcctgaaa
     3181 atgatgacgg tctaccgagc tttcgccttg agcatttaac caaagcgaat ggtattgaac
     3241 atagcaacgc ccacgatgcg atggctgatg tgtacgccac tattgcgatg gcaaagctgg
     3301 taaaaacgcg tcagccacgc ctgtttgatt atctctttac ccatcgtaat aaacacaaac
     3361 tgatggcgtt gattgatgtt ccgcagatga aacccctggt gcacgtttcc ggaatgtttg
     3421 gagcatggcg cggcaatacc agctgggtgg caccgctggc gtggcatcct gaaaatcgca
     3481 atgccgtaat tatggtggat ttggcaggag acatttcgcc attactggaa ctggatagcg
     3541 acacattgcg cgagcgttta tataccgcaa aaaccgatct tggcgataac gccgccgttc
     3601 cggttaagct ggtgcatatc aataaatgtc cggtgctggc ccaggcgaat acgctacgcc
     3661 cggaagatgc cgaccgactg ggaattaatc gtcagcattg cctcgataac ctgaaaattc
     3721 tgcgtgaaaa tccgcaagtg cgcgaaaaag tggtggcgat attcgcggaa gccgaaccgt
     3781 ttacgccttc agataacgtg gatgcacagc tttataacgg ctttttcagt gacgccgatc
     3841 gtgcagcaat gaaaattgtg ctggaaaccg agccgcgtaa tttaccggca ctggatatca
     3901 cttttgtcga taaacggatt gaaaagctgt tgttcaatta tcgggcgcgc aacttcccgg
     3961 ggacgctgga ttatgccgag cagcaacgct ggctggagca ccgccgccag gtcttcacgc
     4021 cagagttttt acagggttat gcagatgaat tgcagatgct ggcacaacaa tatgccgatg
     4081 acaaagagaa agtggcgctg ttaaaagcgc tttggcagta cgcggaagag atcgtctaat
     4141 ttgaagcctc cgccgctggt acggcggagg acaatgttga gtcaggcttt ttgaacggtg
     4201 atgctccacg ctgcatcgcc aatctgctga taatcggtga tggcatgtcc ttcttctgcc
     4261 gcccactgtg ggattgcttc agttgcctgg gtgcaatcaa actcaattac caactcatcg
     4321 ccgctggcca tttctgccag tgctgctttt gcttctatga gtggaaacgg acagacctgc
     4381 gtcaccacat ccagcttttt aatcgccatc tttacacctt acttaatttg ccgcagcagt
     4441 tgccagtcgc gccttacgct gcggtcgaac atacacaagc caggacgcag tccagactcc
     4501 gagaatcata aatacaaggc caatccagcc ctgccaggtc atcattgcag tcataaccag
     4561 tccattaccg atagagcaac cacctgcaat actggcaccg aagcccatca gtacaccacc
     4621 taacccgcta cgtaatgttg tttgtgcatc agctgcgcga acgcggaact cacggctcgc
     4681 tttggctgca ataaatgacc ccacgaagat ccctaatacc aggaaaacgc cccagttaat
     4741 gtatttcacg tcacccgcga ccagaaattg cagaatattg gccgttgggg aagtgattcc
     4801 taacccaaac atgcgtccgg ttgcttcact caggggccag gctaaaagcg caatcaaacc
     4861 gatgagtaca gcagtgacaa agggatgcca gcgtttttca aacagaatat gagcgatccc
     4921 ggttcggcgc ggcggtaagg tcgcgacttt gagttttggc ttcttcaact ctttcatcac
     4981 cacccagagc gttatcacca gcaaaacggc aaccaacggc cacacagaca aattaaaagt
     5041 ctcagcaata gagttatgtt cagtactgta gtgctgcaag gtttgattta aaccactggc
     5101 atgtggagaa cgcatcaccg cactcatcac catataagtg aaaagcgcga tccaactgcc
     5161 gatcaatccc tcacccgcgc gataccaggt cccggtggca catccacccg ccagaacaat
     5221 tcccagcccg aagatatacc cacctataac agtaccaagc cacgggaacg ccccggcttc
     5281 gtaagtcagt aggcccgcct gaattaacgc aaaaaccccc acgctttgta ccgaaattgc
     5341 aatcagcaag gcgtaaaaca tgcgattatt tttcacgata tacatatcgc gaaaaccacc
     5401 tgtcaggcaa aaacgcccac gctgcatgac aaatcccagc agagcaccac aaattagccc
     5461 gcttaatatc attgaaaaca taaattaatt aaccagatga atgttaatag ggaaattatt
     5521 catgactggt ggaatgcaga ccaataacca aattctctaa attagaacaa atggttatta
     5581 atgaggatgt cgataaaaaa gaataaaaaa accggagcca aatattcatt cgactccggt
     5641 ttattattac aaaagatgat tacgctacgt cttcgtactg cggaaccggg ttacggaaac
     5701 tcttggtgac gcaagccagg taaatcagac cgatagccgc ccagatcaga cccagaacca
     5761 ttgagctttc ttccaggtta agccacagcg caccaacggt cagcgcacca cacatcggca
     5821 ggaacagata ctggaagtga tctttcagcg tcttattacg cttctcacgg atccagaact
     5881 gcgagatgac cgacaggtta acgaaggtga acgccaccaa cgcaccaaag ttaatcagcg
     5941 ctgtagccat taccaggtcg aagttgattg ccagcagggc aatcgcgcca accaggatga
     6001 tgttcatcgc cggagtacgc catttcgggt gtacataacc gaagaagctt ttcgggaata
     6061 cgccgtcacg acccattacg tacatcagac gcgctacgcc tgcatgcgcc gccataccgg
     6121 acgccagtac ggtaatggtg gagaagatca gcgcgccaac ctggaacgct ttacccgcaa
     6181 cgtacagcat gatttcaggc tgtgacgcat ccggatcttt aaagcgagag atatccggga
     6241 agtacagctg caggaagtaa gttgcaaaga tgaagatcat gccgccaatc agcgcggtca
     6301 ggaaaatcgc acgcgggatc acgcgctctg catctttggt ttcttccgac aggttgctga
     6361 tgccgtcaaa gccggtaaag gagaagcaca ggattgtcgc cccggtaatc atcgggataa
     6421 catgcgcatc gccagaccag aacggacgag tgctcgccag cgtaccagcg ccttcacctt
     6481 caaatacgcc ataaacaacc atgcccagaa tcaccgcgat cagcactacc tgcaacacga
     6541 cgattacagt gttgaagttc gctacggatt tcagactacg caggttaaag gcggtcatga
     6601 aggccaccag cgccaccaca aacatccacg atgggatgga aggcactaga gcttcaaaat
     6661 agattttcgc cagcagaatg ttgatcatcg gcgcgaacag atagtcgagc agagaagacc
     6721 aacccaccat aaagccgaca gtcgggctaa tggatttctg ggcgtaagtg tatgcagagc
     6781 cagcagaagg atagcggcga accagcttcc cgtagctcag agccgtaaac aggatcgcaa
     6841 tcaatgcgaa cgcataggct gtcggcacat gaccatccgt gaggcctgaa acgataccaa
     6901 aagtatcaaa cagcgtcatc ggctgcatat aggcaagacc catcattaca accggaacta
     6961 acgtaagcgt tttacgcaat tccacgcgag aggtgtttgg agtaacgtta tgcgacatgg
     7021 tcattctcct ttacggtgat aaccgtcgcg taagcaaaaa attgccccat ttttttggat
     7081 tcctcagcga caacaactgt cgatttttag taaatatcta tccggtacga agcccggcct
     7141 cttggtatga atgattggtt tgaagcaaaa aataaccgac gctgatgaaa cgtcggtttt
     7201 tagtcatttt ttgacagcgg cgcattgtgc ctaaatgggg gggaaatgac aagagaatga
     7261 gaggcttgtc ataataattt ttctttaaat ggctgatttt ccgtcatcag attttgcata
     7321 aacaccggcg atcgcactat ttgctaaaat ttcatcccgc caccagccgc tggcaagccc
     7381 tgcggtctgt tcattccagc caatccagac gggttcaaag agagtttggg cgaccacttt
     7441 cttctcgatt aacgcgccac tatcgagaaa ccgttgcgcc agataacggg gtaaatagcc
     7501 gcagccgagg ccgctaattt gcagctccag cttggtttta aaatcgaaaa cggtaatggc
     7561 ttcttgttcg tcgagaagtt gtgaggccgt agaagcgcct gcctgcgcag tatcccccac
     7621 cacaatcgcc cgatatcgct taataatacg acggtttaac ggctcttctt ccagagctaa
     7681 cggatgatga ggcgcgaccg caaagacttg ttccagatcg cccaaccgtg aaaaaccaaa
     7741 ctcactggaa gatggtggct catgcatcgc gccaacaata atatccgctc gcccctgagt
     7801 cagggcatcc caggaaccac cgagtacgcc attgataaac ttcaggcgcg tcacgctatg
     7861 atgttgataa aaagcttcaa tcagtggcgc aagcagagaa aaaggaaacg tgtcgtccac
     7921 gccaatcacc agctcgtttt cccagccttc atgcagtttg atcgcctgct tttccagttc
     7981 tcgcacggta tgcagaactt cccgcccttt ctctaataac atttttccgg tacgggtgaa
     8041 tttagcgcgg tgaccgctac gatcaagcaa ttgaatatta aggtcgcttt ccagcttgtg
     8101 aacggtgtaa ctcagagcgg acggcgtctt atagagtttg gccgacgccg ccgcaaaact
     8161 gccttctttt tctaaggcat cgaggatcat caacacatcc agcagtggtt tcatacacgc
     8221 ccccttgcag tgtatgcggc gtcctgctgg cgtgatcgtt tactccagcg gcattaccag
     8281 cggatcggga tactggtatt caaatcccag ttcattacaa atccgactgc catcaataat
     8341 cttacctttg ccgctgtcca gactatttct gaactgcgga ggctccagcc ccagtaaacg
     8401 ggccatctgc ggatagaaaa cattacgcgc agggtgagct ggcgcacata tattatagat
     8461 gtgtccgcct ttaggtgcct gtaacaacag agtgatagcg ccaataacat cttctaaatg
     8521 gactaaatta acaccatgtt caccatcagg cgcggttttt ccggcaaaga agcgtccggg
     8581 atgacgtccc ggtccgacca gccccgcaag acgcagaata tcgaccgaag taccgggtaa
     8641 attgtgcagc cagtcttcga gttcttctaa tactcgtcca ctgttggtta ccggattacg
     8701 cggggtagtt tctttcaccg tgccttgcgc gtcgccatag acagatgttg agctggtaaa
     8761 aataatgcgg ggaatacgat gggccagcgc gctatccact aactcttgta ccgcttgtaa
     8821 atagaactca tcgccggggc cgctacgacg tgccggaagc gtaatgacca gcgcatcggc
     8881 atccatcagg gcatccagat cgtcagaatc gcaaactagc tcaggctcca tgcgaagcag
     8941 atagctatca atgccactca ttcgggccgc ttcgacacca tcttgtgtgg ttttactccc
     9001 agtgacttgc cagcctcgcg ctgaaagtga catcgccagc ggcatgccta accaccctaa
     9061 cccgacaatt gcgacctttt tcatccgtta tctcctgata caccttagat ctataaggct
     9121 acgctagcgt atcaaaactg acaattcatt ctatgaatga atctgttcaa taatgataac
     9181 gacatgctgc aatgagcagt gaatcgtcgg taaccgcgta taccagacgg tgctcctctg
     9241 taatgcgtcg ggaccagaaa cctgacaaat tatgtttcag gggttctggc ttccccttac
     9301 cttcaaatgg cgttctgcgg gtatctttga taagttcatt gatcttttta acaattcgct
     9361 tatctgtttc ctgccagtac agataatcat cccatgattc ctcagaccag attagtttca
     9421 ctcaataatg tccttttctg ttcctttgcc tgatttcagg ctatcgattg agtccatcaa
     9481 tctccgggcg ttagcggggg aacgcagtag ataagccgtc tcttccagcg agttgtattc
     9541 ttcgagtgac atcagaacac aagcctctcc attctgacga gtaataagga tcggggcatg
     9601 atcttcaacg gctttcatca ttgttgccga caaattctga cgcgcttcgc tgtagctaat
     9661 tgtacgcatg tcaatctcct cttttgtaca gttcattgta caatgatggg cgttaattaa
     9721 ctatttatta attagtttgt agatcaaggt attgtcagtg agacgaaaat ccaggcttcg
     9781 ctatttttgg tgccgtcagc taagaggaca gtcctcttag ccccctcctt tccccgctca
     9841 ttcattaaac aaatccatcg ccataaaata tataaaaaag cccttgcttt ctaacgtgaa
     9901 agtggtttag gttaaaagac atcagttgaa taaacattca cagagacttt tatgacacgc
     9961 gttcaattta aacaccacca tcatcaccat catcctgact agtctttcag gcgatgtgtg
    10021 ctggaagaca ttcagatctt ccagtggtgc atgaacgcat gagaaagccc ccggaagatc
    10081 accttccggg ggctttttta ttgcgcggtt gataacggtt cagacaggtt taaagaggaa
    10141 taacaaaatg acagacaaca ctcgtttacg catagctatg cagaaatccg gccgtttaag
    10201 tgatgactca cgtgaattgc tggcgcgctg tggcattaaa atcaatcttc acacccagcg
    10261 cctgatcgcg atggcagaaa acatgccgat tgatattctg cgcgtgcgtg acgacgacat
    10321 tcccggtctg gtaatggacg gcgtggtaga tcttgggatt atcggcgaaa acgtgctgga
    10381 agaagagctg cttaaccgcc gcgcccaggg tgaagatccg cgctacttta ccttgcgtcg
    10441 tctggatttc ggcggctgcc gcctttcgct ggcaacgccg gttgatgaag cctgggacgg
    10501 cccgctctcc ttaaacggta aacgtatcgc cacctcttat cctcacctgc tcaagcgtta
    10561 tctcgaccag aaaggcatct cttttaaatc ctgcttactg aacggttctg tcgaagttgc
    10621 cccgcgtgcc ggactggcgg atgcgatttg cgatctggtt tccaccggtg ccacgctgga
    10681 agctaacggc ctgcgcgaag tcgaagttat ctaccgctcg aaagcctgcc tgatccagcg
    10741 cgatggcgaa atggaagaat ccaaacagca actgatcgac aaactgctga cccgtattca
    10801 gggtgtgatc caggcacgcg aatcaaaata catcatgatg cacgcaccga ccgaacgtct
    10861 ggatgaagtc atcgccctgc tgccaggcgc cgaacgccca actattctgc cgctggcagg
    10921 tgatcaacag cgtgtagcga tgcacatggt cagtagcgaa accctgttct gggaaacgat
    10981 ggaaaaactg aaagcgctgg gtgccagttc aatcctggtc ctgccgattg agaagatgat
    11041 ggagtgatcg ccatgagctt taacacaatc attgactgga atagctgtac tgcggagcaa
    11101 caacgccagc tgttaatgcg cccggcgatc tccgcttctg aaagcattac ccgcaccgtt
    11161 aacgatattc tcgataacgt gaaaacgcgt ggcgatgagg ccctgcggga atacagcgcg
    11221 aagtttgata aaaccacggt taccgcgctg aaggtgtctg ctgaggagat cgccgccgcc
    11281 agcgaacgcc tgagcgacga gctaaaacag gcgatggcgg tggcagtaaa gaatattgaa
    11341 accttccaca ctgcgcaaaa actgccgcca gtagatgtag aaactcagcc aggcgtacgt
    11401 tgtcagcaag tcacgcgccc ggtggcttcg gtaggtttgt atattcctgg cggctccgcc
    11461 ccgctcttct caacggtatt aatgctggca actccggcgc gtattgcggg ctgtaaaaaa
    11521 gtggtgctgt gctcaccgcc gccgattgcc gatgagatcc tttatgcggc gcagctgtgc
    11581 ggtgtgcagg acgtgtttaa cgtcggcggc gcacaggcca ttgccgcact ggcgtttggt
    11641 acggaatctg tgccgaaagt ggacaaaatc ttcgggccgg gtaacgcctt tgtcaccgaa
    11701 gcgaaacgtc aggtgagcca gcgtctggac ggtgcggcta tcgatatgcc cgcaggcccg
    11761 tcggaagtgt tggtcattgc cgacagtggc gcaacgccgg atttcgtggc ttctgatttg
    11821 ctttctcagg ctgaacacgg cccggactca caggtgattt tactgacgcc cgatgctgat
    11881 atggcgcatc aagttgccga ggccgtcgaa cgccagttgg cagaactgcc gcgtgccgaa
    11941 accgcccgtc aggcacttag cgccagccgc cagatcgtga ctaaagattt agcgcagtgc
    12001 gtagagatct ccaaccagta cggcccggag cacctgatca ttcagacccg caacgcccgt
    12061 gaactggtcg atagcattac cagtgccggt tcggtatttc ttggtgactg gtcaccggaa
    12121 tcggcaggtg attacgcctc cggcaccaac cacgttctac cgacttacgg ttacaccgcc
    12181 acctgttcca gcctcgggct ggcagatttc cagaagcgca tgaccgtgca ggaactgtcg
    12241 aaagaaggct tttctgcgct ggcttcgacc atagaaacac tggccgccgc cgagcgcctg
    12301 accgcccata aaaatgccgt tactttgcgt gttaacgccc ttaaggagca agcatgagca
    12361 ccgtgactat taccgattta gcgcgtgaaa acgtccgcaa cctgacgccg tatcagtcgg
    12421 cgcgtcgtct gggcggtaat ggcgacgtct ggctgaacgc caacgaatat cccacagccg
    12481 tggagtttca gcttactcag caaacgctca accgctaccc ggaatgtcag ccgaaagcag
    12541 tgattgaaaa ttacgcgcaa tatgcaggcg taaaaccgga acaggtgctg gtcagccgtg
    12601 gcgcggacga aggtattgaa ctactgattc gcgctttttg cgaaccgggt aaagacgcca
    12661 tcctctactg cccgccaacg tacggcatgt acagcgtcag cgctgaaacc attggcgtcg
    12721 agtgccgcac agtgccgacg ctggaaaact ggcaactgga cttacagggc atttccgaca
    12781 agctggacgg cgtaaaagtg gtctatgttt gcagccccaa caacccgacc gggcaactga
    12841 tcaacccgca ggattttcgc accctgctgg agttaacgcg cggtaaggcg attgtggttg
    12901 ccgatgaagc ctatatagag ttttgtccac aggcatcgct ggctggctgg ctggcggaat
    12961 atccgcacct ggctattttg cgcacactgt cgaaagcttt tgctctggcg gggcttcgtt
    13021 gcggatttac gctggcaaac gaagaggtca tcaacctgtt aatgaaagtg atcgccccct
    13081 acccgctctc gacgccggtt gccgacattg cggcccaggc gttaagcccg caggggatcg
    13141 tcgccatgcg tgagcgggtg gcgcaaatta ttgcagaacg cgaatacctg attgccgcac
    13201 tgaaagagat cccctgcgtg gagcaggttt tcgactctga aaccaactac attctggcgc
    13261 gctttaaagc ctccagtgca gtgtttaaat ctttgtggga tcagggcatt atcttacgtg
    13321 atcagaataa acaacccttt ttaagcggct gcctgcgaat taccgtcgga acccgtgaag
    13381 aaagccagcg cgtcattgac gccttacgtg cggagcaagt ttgatgagtc agaagtatct
    13441 ttttatcgat cgcgatggaa ccctgattag cgaaccgccg agtgattttc aggtggaccg
    13501 ttttgataaa ctcgcctttg aaccgggcgt gatcccggag ctgatgaagc tgcaaaaagc
    13561 gggctacaag ctggtgatga tcactaatca ggatggtctg ggaacacaaa gtttcccgca
    13621 ggcggatttt gatggcccgc acaacctgat gatgcagatc ttcacctcgc aaggcgtgca
    13681 gtttgatgaa gtgctgattt gtccgcacct gcccgccgat gagtgcgact gccgtaagcc
    13741 gaaagtaaaa ctggtggagc gttaccttgc tgagcaagcg atggatcgtg ccaacagtta
    13801 tgtgattggc gatcgcgcga ccgatattca actcgctgaa aacatgggca ttactggttt
    13861 acgctacgac cgcgaaaccc tgaactggcc gatgattggt gagcaactca ctaaacgaga
    13921 ccgttacgcc catgtagtgc gcaacaccaa agagacgcag attgacgttc aggtgtggct
    13981 ggatcgcgaa ggtggcagca agattaacac cggtgttggc ttctttgatc acatgctgga
    14041 tcagatcgcc acccacggcg gtttccgtat ggaaatcaac gtcaaaggcg acctctatat
    14101 cgacgatcac cacaccgtcg aagataccgg cctggcgctg ggcgaagcgt taaaaattgc
    14161 cctcggcgat aaacgcggta tttgccgctt tggttttgtg ctgccgatgg acgaatgcct
    14221 tgcccgctgt gcactggata tctctggtcg cccgcacctg gaatataaag ccgaatttac
    14281 ctaccagcgc gtgggcgatc tcagcaccga aatgatcgag cacttcttcc gttcgctctc
    14341 atacaccatg ggcgtgacgc tacacctgaa aaccaaaggt aaaaacgatc accaccgtgt
    14401 agagagcctg ttcaaagcct ttggtcgcac cctgcgccag gccatccgcg tggaaggcga
    14461 caccctgccc tcgtcgaaag gagtgctgta atgaacgtgg tgatccttga taccggctgc
    14521 gccaatctga actcggtgaa gtcagccatt gcgcgccacg gttatgaacc aaaagtcagc
    14581 cgtgacccgg acgtcgtgtt gctggccgat aaactatttt tacccggcgt tggcactgcg
    14641 caagcagcga tggatcaggt gcgtgagcgc gagctgtttg atctcatcaa agcctgtacc
    14701 caaccggtac tgggcatctg cttagggatg caactgctgg gtcggcgcag cgaagagagc
    14761 aacggcgtcg acttgctggg catcattgac gaagacgtgc cgaaaatgac cgactttggt
    14821 ctgccactgc cgcatatggg ctggaaccgc gtttacccgc aggcaggcaa ccgcctgttt
    14881 caggggattg aagacggcgc gtacttttac tttgttcaca gttacgcaat gccggtcaat
    14941 ccgtggacca ttgcccaatg taattacggc gaaccgttca ccgcggcggt acacaaagat
    15001 aacttctacg gcgtgcagtt ccacccggag cgttctggtg ccgctggcgc taagttgctg
    15061 aaaaacttcc tggagatgtg atgattattc cggcattaga tttaatcgac ggcaccgtgg
    15121 tgcgtctcca tcagggcgat tacggcaaac agcgcgatta cggtaacgac ccgctgccgc
    15181 gcttacagga ttacgccgcg cagggtgccg aagtgttgca tctggtggat ctgaccgggg
    15241 caaaagatcc ggctaaacgt caaatcccgc tgattaaaac cctggtggcg ggcgttaacg
    15301 ttccggtgca ggttggcggc ggcgtgcgta gcgaagaaga cgtggcggcg ttactggaag
    15361 cgggcgttgc gcgcgtggtg gtcggctcca ccgcggtgaa atcaccagaa atggtgaaag
    15421 gctggtttga acgtttcggt gccgatgcct tagtgctggc gctggatgtc cgtattgacg
    15481 agcaaggcaa caagcaggtg gcagtcagcg gctggcaaga gaactcgggc gtttcactgg
    15541 aacaactggt ggaaacctat ttgcccgtcg gcctgaaaca tgtgctgtgt accgatatct
    15601 cgcgcgacgg cacgctggca ggctctaacg tctctttata tgaagaagtg tgcgccagat
    15661 atccgcaggt ggcatttcag tcctccggcg gtattggcga cattgatgat gtcgctgccc
    15721 tgcgtggtac tggtgtacgc ggcgtaatag ttggtcgtgc attactggaa ggtaaattca
    15781 ccgtgaagga ggccatcgca tgctggcaaa acgcataatc ccatgtctcg acgttcgtga
    15841 tggtcaggtg gtgaaaggcg tacagtttcg caaccatgaa atcattggcg atatcgtgcc
    15901 gctggcaaaa cgctacgccg aggaaggtgc agacgaactg gtgttctacg atatcaccgc
    15961 ttccagcgat ggccgtgtgg tagataaaag ctgggtatct cgcgtggcgg aagtgatcga
    16021 cattccgttt tgtgtggcgg gtgggattaa gtctctggat gacgccgcca aaattctttc
    16081 ctttggcgcg gataaaatct ccattaactc ccctgcgctt gcggacccga cgttaattac
    16141 tcacctggcg gatcgctttg gcgtgcagtg tattgtggta ggtattgata cctggtacga
    16201 cgctgaaacc ggtaaatatc atgtgaatca atataccggc gatgaaagcc gcacccgcgt
    16261 cactcaatgg gaaacgctcg actgggtaca ggaagtgcaa aaacgcggtg ccggagaaat
    16321 cgtcctcaac atgatgaatc aggacggcgt gcgtaacggt tacgacctcg aacaactgaa
    16381 aaaagtgcgt gaagtttgcc acgtcccgct gattgcttcc ggtggcgcgg gcaccatgga
    16441 acacttcctc gaagccttcc gcgatgccga cgttgacggc gcgctggcag cttccgtatt
    16501 ccacaaacaa ataatcaata ttggtgaatt aaaagcgtac ctggcaacac agggcgtgga
    16561 gatcaggata tgttaacaga acaacaacgt cgcgaactgg actgggaaaa aaccgacgga
    16621 ctgatgccgg tgattgtgca acacgcggta tccggcgaag tgttaatgct gggctatatg
    16681 aacccggaag ccttagacaa aaccatcgaa agcggcaaag tcaccttctt ctcgcgcact
    16741 aaacagcgac tgtggaccaa aggcgaaacg tcgggcaatt tcctcaatgt ggtgagtatt
    16801 gccccggact gcgacaacga cacgttactg gtgctggcaa atccaatcgg gccaacctgc
    16861 cacaaaggca ccagcagctg cttcggcgac accgctcacc agtggctgtt cctgtatcaa
    16921 ctggaacaac tgctcgccga acgtaaatct gccgacccgg aaacctccta caccgccaaa
    16981 ctgtatgcca gcggcaccaa acgcattgcg cagaaagtgg gcgaagaagg cgtggaaacc
    17041 gcactggccg cgactgtaca tgaccgcttt gagctgacca acgaggcgtc tgatttgatg
    17101 tatcacctgt tggtattgtt gcaggatcag gatctggatt taacgacggt gattgagaac
    17161 ctgcgtaaac ggcatcagtg agttgcgggg taagcggatg cgatattgtt gtcgcatccg
    17221 gcaaaaaaaa acgggcaagg tgtcaccacc ctgcccattt tctttaaaac cgaaaagatt
    17281 acttcgcgtt gtaattacgc agagcgttac gccccaacac aatccccgcg ccaaccatac
    17341 cgcccagcag caccgccaga atcaaggtaa ttgccttttt cgggctatcg cgacgaatag
    17401 gtaacgtcgg tttcattaca tagcggtaag catgaatatc aagtttatca acgtcaagat
    17461 tgtcgatatc cagcaggttt tgacgagtct gatagtagtt tgatgagaac accaacggac
    17521 gagtcgcctc gtgcttaatc atcgactcca gcgcttcgct ccccaaaagg aacatagtat
    17581 cctgggttac gtcctgtgtc tgctgaatct gcggctttgt cacctgcgcc tgattcgcat
    17641 actgcaacgc ttcctgaatc tgacggatac gcagatcttt ctgctcctgc gcaaccactt
    17701 cctgggtctt cagagaatcc tgcaagtttt tcatttgcag cgcgatgttg tctttcaggt
    17761 cttgttccag ttcttcattt acctggtcgt caacttgctg gatatactgc gccagctgtt
    17821 tctgcgcacc ttcaggtgtt tcacccacat aagaaaccgc taaaggtaat gactgatttt
    17881 taacggtagg ctcaatggtc agtttttcag gttcttcctg attatccagc gtttctgcta
    17941 atgcagaaaa tgtcgtgctg aaacgaccaa ttaacatcga ctggatctca gacactttcg
    18001 gcgctgccga accataaatc acatttatgg cattgttata accagcgatt tgtcccacat
    18061 caggttgagt aacgatagct gtagaagtcc acttttcttt ggcaacggcc agatacccta
    18121 tagccagggc aatagcgaca atgacagaga tgataattgt catcttgcca cgccacaact
    18181 gcaccagtaa atcaatcaaa tcaatctgtt ccgggtcatg gttttgccca gaaacattat
    18241 tattttctac tctcatcatt accctaacgg acgaaataat tttaagtcgc catagtttag
    18301 cgataatcgc catgaatgct ataggataaa tgataaaaat aatgaattat tacaaaggga
    18361 gcataagaga atcagggcga aaatcgccct gataacagga tgttagtcac tgccaaagag
    18421 atcgcgggtg tagactttgt ctgccacatc cttaagctct tctgccatac ggttggagat
    18481 aatgacgtcg gcttgttgtt tgaaggtggc gagatcacgt tccaggcgag agttgaagaa
    18541 tgagtcttct ttcatcactg gctcgtatat gatcacttca acgcctttcg ccttgatacg
    18601 tttcataatc ccctgaatgg aagacgcacg gaagttatct gatccgctct tcataatcag
    18661 acgataaata cccactactt gcggcttgcg tgacaaaatg gcatcggcaa taaaatcttt
    18721 acgtgtacgg tttgcatcga caattgccga aatcaggtta ttcggcacag actggtagtt
    18781 cgccagtaac tgcttggtat ctttcggcag acaataacca ccataaccaa acgacggatt
    18841 gttgtaatgg ttgccaattc gcggatcgag acaaacgcct tcgattattt gacgggaatt
    18901 cagacctaaa ctttctgcgt agctatccag ttcgttaaag tacgccacgc gcatcgccag
    18961 gtaggtgttt gcaaaaagtt taatcgcttc tgcttcagtg gagtcggtaa acagggtagg
    19021 gatattttgc ttaatcgctc cttcctgtaa taacgcagcg aaacgttctg cgcgttctga
    19081 acgctcacca atgacaatac gtgaaggatg gagattatcg taaagggctt taccttcacg
    19141 gagaaattcc ggagagaaaa taaggttatc gatacccaga cgttctttaa ttgatttggt
    19201 gaaacctacc ggaattgtag atttaatcac catgacagca ttaggattaa tttctgtcac
    19261 atcgcgaatg accgcttcca cgcttgaggt attaaaataa tttgttttcg ggtcataatc
    19321 ggtaggcgtg gcaataataa cgtaatcggc atttttatac gcgtcatact tatctgtcgt
    19381 agcgcggaaa ttgagatctt tagtcgccag atactcttca atctccttat caacaatcgg
    19441 tgactgcttc ttgttaagca tgtccacttt ggcctgaacg atatccagtg caaccacttc
    19501 gtgattttgc gcaatcagaa taccatttga aagaccaaca taacctgttc ctgaaattgt
    19561 tattttcatt agctctgact tcttccggtt aaacatttag agtggtcatt aatccaccac
    19621 cacaacttca tctactgcgg gatttttaac gctgatgtag atgaactgtc aaggcagcga
    19681 tcctgctgtg cggcgctgta ttatatcgcg tttttaacta taaattataa aaaaaggcca
    19741 taacatgccg ctttgtataa taaaaaagcc cggagggttt ctccgggcct tgcattgatt
    19801 aattgattta aatcagatta atccagccat tcggtatgga acacaccttc tttatcaatg
    19861 cgcttataag tatgcgcacc gaaatagtca cgctgtgcct ggatcaggtt cgcaggcaga
    19921 acagcggcgc ggtagctgtc gtaataggca accgcagcag cgaaggtagg aacagggata
    19981 ccgttctgta ctgcataagc aacgacatca cgcagcgcct gctggtagtc atcggcaatt
    20041 tgcttgaagt acggagccag cagcaggtta gcgatctgcg gattttcggc ataagcatcg
    20101 gtgattttct gcaggaactg cgcacggatg atgcagccag cacggaaaat cttcgcgatt
    20161 tcgccgtagt tcagatccca gttgtactct tcagacgccg cacgcagttg agagaacccc
    20221 tgagcgtaag aaacgatttt gcccagatac agtgcacggc gaactttttc gatgaactca
    20281 gccttgtcgc cagctagctg cgcttgtggg ccagagagaa ctttagacgc agcaacgcgc
    20341 tgatctttca gagaagagat gtatcgtgca aacacagact cggtaatcag cgacagcggt
    20401 tcgccgagat ccagtgcgct ctggctggtc catttgcccg tacctttgtt tgccgcttca
    20461 tccaggatca catcaaccaa gtagttaccg tcttcatctt ttttagtgaa gatgtctttg
    20521 gtaatgtcga tcaggtagct gctcagttca ccgttattcc actcggtaaa ggtctgcgcc
    20581 agttcttcgt tggtgaggtt cagaccacct ttaagcagag aataggcttc agcaatcagc
    20641 tgcatatcgc cgtactcaat accgttgtga accatcttca catagtgacc tgcgccatcg
    20701 gcaccaatat aggtaacgca cggttcgctg tcttcagcca ctgcggcgat ttttgtcagg
    20761 atcggcgcaa ccagttcata ggcttctttc tgcccaccag gcataatgga aggacctttc
    20821 agcgcgcctt cttcaccacc ggagacaccg gtaccaatga agttaaagcc ttctgcagaa
    20881 agctcacggt tacgacggat ggtgtcctgg aagaaggtgt taccaccatc aatgatgatg
    20941 tcgcctttat cgaggtatgg cttgagagaa tcaatagcag catccgtgcc tgcacctgct
    21001 ttcaccatta acaggatgcg acgaggcgtt tccagagatt caacaaactc tttcaccgta
    21061 tagtaaggaa ccagtttctt gcctggattt tcggcaatca cttcttccgt tttctcacgg
    21121 gaacggttga aaatagagac ggtataacca cggctttcga tgttgagcgc aaggttgcgc
    21181 cccatcactg ccataccgac gacgccgatc tgttgctttg acattgttta ctcctgtcag
    21241 ggggtgttca ccgcagggcg gttatggata gtaataaact tattaattca tagtttacaa
    21301 aaactatttt ttattagtag cagatatgcc gaccgttttc aaataagtaa aaattgagta
    21361 ataaaaaatc atggagaatg ctag
//
LOCUS       DAZGVQ010000085        21362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000085 DAZGVQ010000000
VERSION     DAZGVQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.086"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            45..569
                     /locus_tag="ACHLAG_003051"
     CDS             45..569
                     /locus_tag="ACHLAG_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HFP0656237.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            653..1339
                     /gene="lpfB"
                     /locus_tag="ACHLAG_003052"
     CDS             653..1339
                     /gene="lpfB"
                     /locus_tag="ACHLAG_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFP0656238.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQCQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            1363..3894
                     /gene="lpfC"
                     /locus_tag="ACHLAG_003053"
     CDS             1363..3894
                     /gene="lpfC"
                     /locus_tag="ACHLAG_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFP0656239.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3905..4960
                     /gene="lpfD"
                     /locus_tag="ACHLAG_003054"
     CDS             3905..4960
                     /gene="lpfD"
                     /locus_tag="ACHLAG_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFP0656240.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            4966..5490
                     /gene="lpfE"
                     /locus_tag="ACHLAG_003055"
     CDS             4966..5490
                     /gene="lpfE"
                     /locus_tag="ACHLAG_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFP0656241.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5743..7434
                     /gene="eptB"
                     /locus_tag="ACHLAG_003056"
     CDS             5743..7434
                     /gene="eptB"
                     /locus_tag="ACHLAG_003056"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFP0656242.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            7526..7602
                     /locus_tag="ACHLAG_003057"
     tRNA            7526..7602
                     /locus_tag="ACHLAG_003057"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7560..7562,aa:Pro,seq:cgg)
     gene            8347..9954
                     /gene="dppA"
                     /locus_tag="ACHLAG_003058"
     CDS             8347..9954
                     /gene="dppA"
                     /locus_tag="ACHLAG_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFP0656243.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10105..11124
                     /gene="dppB"
                     /locus_tag="ACHLAG_003059"
     CDS             10105..11124
                     /gene="dppB"
                     /locus_tag="ACHLAG_003059"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFP0656244.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRLQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11134..12036
                     /gene="dppC"
                     /locus_tag="ACHLAG_003060"
     CDS             11134..12036
                     /gene="dppC"
                     /locus_tag="ACHLAG_003060"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFP0656245.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12047..13030
                     /gene="dppD"
                     /locus_tag="ACHLAG_003061"
     CDS             12047..13030
                     /gene="dppD"
                     /locus_tag="ACHLAG_003061"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFP0656246.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13027..14031
                     /gene="dppF"
                     /locus_tag="ACHLAG_003062"
     CDS             13027..14031
                     /gene="dppF"
                     /locus_tag="ACHLAG_003062"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFP0656247.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14061..15332)
                     /locus_tag="ACHLAG_003063"
     CDS             complement(14061..15332)
                     /locus_tag="ACHLAG_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFP0656248.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            15808..15915
                     /locus_tag="ACHLAG_003064"
     CDS             15808..15915
                     /locus_tag="ACHLAG_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0656249.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16291..16398
                     /locus_tag="ACHLAG_003065"
     CDS             16291..16398
                     /locus_tag="ACHLAG_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0656250.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16774..16881
                     /locus_tag="ACHLAG_003066"
     CDS             16774..16881
                     /locus_tag="ACHLAG_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0656251.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            17257..17364
                     /locus_tag="ACHLAG_003067"
     CDS             17257..17364
                     /locus_tag="ACHLAG_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0656252.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(17451..19130)
                     /gene="bcsG"
                     /locus_tag="ACHLAG_003068"
     CDS             complement(17451..19130)
                     /gene="bcsG"
                     /locus_tag="ACHLAG_003068"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFP0656253.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(19127..19318)
                     /gene="bcsF"
                     /locus_tag="ACHLAG_003069"
     CDS             complement(19127..19318)
                     /gene="bcsF"
                     /locus_tag="ACHLAG_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFP0656254.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(19315..20886)
                     /gene="bcsE"
                     /locus_tag="ACHLAG_003070"
     CDS             complement(19315..20886)
                     /gene="bcsE"
                     /locus_tag="ACHLAG_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFP0656255.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            21159..21347
                     /gene="bcsR"
                     /locus_tag="ACHLAG_003071"
     CDS             21159..21347
                     /gene="bcsR"
                     /locus_tag="ACHLAG_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFP0656256.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT         5429 a         5182 c         5610 g         5141 t
ORIGIN      
        1 atgaacaaca tcaagcatat ttatctatat aaatggagtt ataaatgaaa aaggttctgt
       61 ttgccttatc tgcgcttgcg ctgacctcta cttccgtatt cgcagctgat gcgggcgacg
      121 gtactgttaa attcaccggt gagattgttg acgccgcttg tgttgtgtct ccagacactc
      181 aaaaacaaga agttgttctg ggtcaggtga acaagtctgt atttactgct atgggtgata
      241 aatccacagc aacgccgttc aaaatcaaac tggaaaactg cgatatctcc actttcaaaa
      301 atgtggaaat cagctttaac ggcgttggcg atgcagacaa cagcaaactg gtttctgtaa
      361 gcactgaacc aggtgccgca actggcgtag gtatcggtat ttatgataac accaataccc
      421 tggttgacct gaatactggt aaatctgcta ctgtcctgaa agaaggtcag accgttctgt
      481 atttcaccgc taactatgtc tctaccaaag atgcagtaac cacgggttac ggtaacgctg
      541 aagtcgactt caacctgact tacaactaat cgatcctgac ggaacgatca tctctcaatg
      601 gcaacagaga ctgtgttgcc attattcaca ccaatgactc agggaccgaa tcatgaaacg
      661 tattagcttt gctgtgctgg cacttttaat gaccgcacaa cagtgttttg ctggtggcgt
      721 agccttaagc acgacgcgtg ttatttatga tggcggcaag aaagaagcgt cgctgaccgt
      781 tgaaaaccat aataaaaatg aagagttttt gatccagtca tggattgatg atgtgaatgg
      841 caataaaaaa acaccgttta ttattacacc gccattattc aaacttgatc ctgataaaaa
      901 taacgtctta cgtatcgtca atattgaaga taccttacct aaagatcgtg aatccgttta
      961 ctggattaat gtaaaggcca ttcctgcgaa aagtgctgaa aatgaaaata aaaacgtctt
     1021 acagatagcc gttcgcaccc gtttgaagtt gttctatcgt ccaaaaggtt tgccgggaag
     1081 caacctcgag gccatgaaac agttacgttt ccagtgtcaa aataaccaaa ttgtggttaa
     1141 taacgactcc gcttataacc tgacctttaa tcagttttcc attaatggac aaaagattga
     1201 acgggcggga atggttctcg cgaaaggaaa gttaaacatt aaccttccag ccggtacagg
     1261 caacgcgcac gaagtgaaat acagcattat taatgatttc ggtgttgctg gcgaaatgct
     1321 gacgaagccg attaactgat ccttttacaa ggcgctttta ctatgcattg gatgcattta
     1381 cctctctatc actacagagc gcatttttcc ttctctctgc tggcattaac gattgccagc
     1441 tcgttacctg cgtatggcgg aaaatttaac cctaaatttc tcgaaaacgt tcagggtatc
     1501 gatcaacaca tcgacttgtc ggtatacgac tcacctgtcg gccagcaaat ccccggtaaa
     1561 taccgggttt cggtgtttgt aaacgaagaa aaaatggctt cccggacgct ggattttagt
     1621 accgccagcg aagcgaaacg caaagccagc ggtgagtcgc ttatgccttg ccttagccgg
     1681 gtgcaactgg aagaaatggg cgtgcgcgtt gacagctttc cggcgctgaa gatgtcacca
     1741 ccagaagcct gcgtagcgtt tgatgaaatc attccccagg ccaccagccg ctttgatttt
     1801 aatactcaga cgctgcatct ttcctttcca caggcggcaa tgatgatgac cgctcgcggc
     1861 acggttgatc cttctcgctg ggacgaagga atacctgcgc tgttgctgga ttacagcttt
     1921 agtggcagca atggacgcaa tgaaggtaca ggttcatctt cagattcgac cagtgacagt
     1981 tattacctga acctgcgcag cggtttgaac gttggtccgt ggcgtttacg taataacagt
     2041 atctggaacc gtaccgatgg caaaaaccaa tgggataacg ttggtacatc gttaaaccgg
     2101 gcaattattc cacttaaatc acagataact ctgggtgata ccgccacgcc tggcgagatt
     2161 tttgatagcg tacaaatgcg tggggcgtta ctggcatccg acgatgaaat gttgccggac
     2221 agccagcgtg gctttgctcc ggtagtgcgc ggcatcgcca aaagcaacgc tgaagtgagt
     2281 atcgaacaga acggctatgt catttatcgc acttttgttc agccgggtgc atttgagatt
     2341 aacgatctct acgcaacctc cggcagcggc gacctgacgg tgatcattaa agaagccgac
     2401 ggaagtgaac aacgttttat ccagccgttt tcggcggtgg cgattttcca gcgtgaaggg
     2461 tatctgaaat acagtctggc tgccggtgaa tatcgtgctg gtaattacga cagcgataaa
     2521 ccacatttcg ggcagtttac cgccatgtac ggcttgccgt ggggtatgac cgcctacggt
     2581 ggtgcattgt tgtcggcaga ttacaacgct ttagctctgg gcctggggaa aaacttcggt
     2641 accattggcg cagtatcggt ggatgtcact caggcgaaaa gtcagttacg caacaatgaa
     2701 aaagaggaag gccagtctta tcgcttcctc tattcgaaaa gttttgaggg tggcactgac
     2761 ctgcgtctgt tgggctacaa atactcaacc agcggctact acaccttcca ggaagcaact
     2821 gatgttcgca gtgacgccga cagtgattac cgccgttatc acaagcgcag ccagatacag
     2881 ggcaatatta cccagcaact gggagactac ggctcggttt atttcaatat gacgcagcag
     2941 gattactgga acgttgacgg taaggaaaac tccttaagcg ccggttatca cggtcacatt
     3001 ggtcgggtga attacagcgt ggcctatacc tggacgagaa gtccggaatg ggaagaagac
     3061 gatcgcttgt ggtcattcag tgtttccatc cctctggggg gcgcgtggag cagttatcgc
     3121 atgactaccg accagaatgg caagacgtcg caacaggcct ctgttagcgg cacgttactg
     3181 gaagatcgta acctgagcta taacgttcag cagggttaca ccagtaatgg cgtaggctac
     3241 agtggcagcg tcaacatggg gtatatgggt ggctctggta acattgacgt aggctataac
     3301 tattctaaag ataaccaaca ggttaactat ggcgtgcgcg gcggtgtcat cgttcacagt
     3361 gaaggtataa cgctttcgca gcctttaggc gaaagtctgg cgattgtttc tgcaccgggc
     3421 gcgcgtggtg gacatgtggt taacagcagc ggcgtagaag ttgactggat gggcaacgcc
     3481 gtggtgcctt atctcacacc atatcgtgaa accattgtgg aattgcgtag cgacacgttg
     3541 gggcaaaacg ttgaactaca ggaagcgttc cagaaagtcg tgcctactcg tggtgcagtt
     3601 gtgcgttccc gctttgatac ccgcgtcggt taccgtgtgc tgatgagctt aaaacaggcg
     3661 aatggcaacg cagtaccgtt tggtgcaacc gcagcgttaa ttgatgagag caaacctgcc
     3721 agcagcattg tgggtgaaga aggacagctt tatatcagcg ggatgccaga agagggcgaa
     3781 ttgcaggtga gctgggggaa cgagcaggcg caacgttgcc gtgtgccatt ccgcttgcca
     3841 gagaacaagg acaatgcggc tatcgtgatg gttaatgcgg tatgcgagaa ataaggatat
     3901 ttagatgaaa attaaaaatt tgttggcagg tatggtgctg ttagggacga gcacttttgc
     3961 tggaaatata tgggctgcgg attggggacc atgtcagaca tcaaatgggg tggcccatga
     4021 gtatagtttt gatttcgtac agactatcca ggtaccttca gaaaataagg ctgggaaaat
     4081 attaactcag ccctttgctt taggtacaaa atattccgct tattgtgaat gcccggatcc
     4141 cattccagat aatggagttg tgacttattt taaaggtgta acgcttctgc ctgagcctgg
     4201 aactgtagat gggtactata aattcaataa ttacattgat attctcacca aaatatatgt
     4261 ctacacacaa ttagatatcc cggtgccttt taccgatcga agcaatggca cggcacagaa
     4321 agaatgtacg ccctatactg ctaacaattg ggggacaggt ggtaaaggaa gtatcagtat
     4381 ttatatttca catccttttg ttgggcaaat gattattcct aaaacacgag ttgcgtccct
     4441 ctttgggaca aaaaagaaag gtgtttacaa tgatagtcag cccatggcca atgtcagtat
     4501 ttccgggagt attacagtta ctcaaggctg tgaacttgcc gctggcaccg tactggatat
     4561 tccattcgga gaatatcagg cccatgattt taaagggcgt gccggacaac caccacaaaa
     4621 tgttcagaag gtacaaaaag aattaagctt tgactgcaat aatatttcag atggcgtcaa
     4681 aatttatctc agtattgatg caacaccaaa tactgcctat cccagcgcaa ttgatttagg
     4741 caacgccgat gttggtgcag tcattgagga cggtaaaggt aatattctta agccgaatga
     4801 cagtaatagc ttattagaaa tgaacccagg ctcgctgtat gaagatgtca aacgcaaagc
     4861 gacaaccaca attaccgctt atcccgttag cactaccgga aaattacccg ctgccgggga
     4921 ttatagcggt attgctacca tacatgtcga actggagtaa cagtcatgaa gcgaacaata
     4981 cttgcgttat gcctgctgcc tctttccttt tcgtcattcg cggcaaccac ggatttaggg
     5041 ccgaaaggaa cattgaaatt cacccttaaa atcagccagg gtgcttgcga actggtgaaa
     5101 gacagcgttg aggttgacat gggcaccgcg gttctcaaaa agccggtaag aatcggaacg
     5161 gaaattaatc ccactccttt ttccatcggc ctgaaaaact gtagcgaagt ggtcagagcc
     5221 tacgtgacta tggacggtac acccaatgca gacgactcaa atctttttgc gctggatgcg
     5281 ggtggcgcta caggcgttgg gctgaaaatc aaaaccgctg ctggagtaca acagttcccg
     5341 gaaaatacaa cgccgacacc ggttgagttt gcaattaacg gtgatggcgc acatcaactg
     5401 aactatatcg ccagctacgt gcctattcgc gccgatgcaa ctattggtcg cgcagatgca
     5461 acggtcgact ttactgtaca gtacgaataa tttcacatct taagggccag ttaactggcc
     5521 ctttttactg ctttttgtaa cgctaaagtc tcttttcaaa cttgcatttt tgtaaatttg
     5581 tgcttcatgc acactctttc cccacacttt ttcgctttgc tgtggtctac ttattcgcgc
     5641 gtgtagattt tacttatctg actacctccg cacttttccc ctgccgggcc tgaaaagccg
     5701 ctaagcaggg tgttatcacc tgtttgtcca gggtttgttt gcatgagata catcaaatcg
     5761 attacacagc agaagctgag ctttttgctt gcaatctata ttggcctttt tatgaatggc
     5821 gcggtttttt atcgccgctt cggcagctat gcgcacgatt ttaccgtctg gaaaggcgtt
     5881 tctgctgttg ttgaactggc cgccaccgta ctggtgacct tctttttact acgtcttcta
     5941 tcgctgtttg gccgccgcag ctggcgtatt ctggcgtcgc tggtggtgct cttttccgca
     6001 ggtgccagct attacatgac cttccttaat gtggtcattg gttatggcat catcgcttcc
     6061 gtcatgacca ccgatatcga cctgtcaaaa gaagttgttg gtctgaactt tattctctgg
     6121 ttaatcgccg ttagtgcatt gcctcttatc cttatctgga ataaccgctg tcgctacacc
     6181 ttgctccgac aactgcgaac cccagggcag cgtattcgca gcctggcggt cgtcgtactg
     6241 gcgggtatta tggtttgggc accgattcgt ttgctggata tccagcagaa gaaagtggag
     6301 agggcgaccg gcgttgattt gccgagttat ggcggtgtcg tagcgaactc ttatctgcca
     6361 tcaaactggc tttctgcgtt ggggctgtat gcctgggcgc gggtcgatga atcttccgat
     6421 aataattcat tgcttaatcc ggcgaagaaa ttcacttatc aggcaccgca aaacgttgat
     6481 gacacttatg tcgtgtttat catcggtgaa accacgcgtt gggaccatat gggtattttc
     6541 ggctatgagc gtaataccac gccgaaactg gcccaggaga aaaatctggc ggcgttccgt
     6601 ggttactcct gtgataccgc aaccaaactc tcactgcgtt gcatgtttgt acgtcagggg
     6661 ggcgcggaag ataatccgca gcgcacatta aaagaacaga acattttcgc ggttctgaag
     6721 cagttaggat tcagttctga cctctacgct atgcagagcg aaatgtggtt ctacagcaac
     6781 acgatggcgg acaacattgc ttatcgtgag cagattggtg cggagccacg taatcgtggc
     6841 aagccggtag atgatatgtt gctggtagac gaaatgcagc aatcgctagg gcgcaacccg
     6901 gatggtaagc atctgatcat tctgcatacc aaaggttcgc attttaacta cacccagcgt
     6961 tatccgcgta gcttcgcgca gtggaagccg gaatgtattg gtgttgatag cggctgtacc
     7021 aaagcgcaga tgatcaactc ctatgacaac tcggtgacct atgtggatca ctttatctcc
     7081 agcgtgattg atcaggttcg cgataagaaa gcgattgtgt tctacgcagc tgaccacggt
     7141 gagtcaatta atgaacgcga gcacctgcac ggcacgccgc gtgaactggc accgccggag
     7201 cagttccgcg taccgatgat ggtctggatg tcagataaat atctggaaaa tccggccaat
     7261 gcgcaggcat ttgcgcagct gaaaaaagaa gccgacatga aagtgccacg ccgtcacgta
     7321 gagctgtacg ataccatcat gggttgtctt ggctatactt caccggatgg tggaattaac
     7381 gaaaacaaca actggtgtca catcccgcag gcaaaagagg cagcggctaa ctaagcggcc
     7441 tgctgacttt ctcgccgatc aaaaggcatt ttgctattaa gggattgacg agggcgtatc
     7501 tgcgcagtaa gatgcgcccc gcattcggtg attggcgcag cctggtagcg cacttcgttc
     7561 gggacgaagg ggtcggaggt tcgaatcctc tatcaccgac caaattcgaa aagcctgctc
     7621 aacgagcagg cttttttgca tctgcagctc atgaggatga gaacctccgg gggcagggag
     7681 gttcgactcg acgtaggcct gataagacgc gtcagcgtcg catcaggcaa tggcaccgca
     7741 cttattgtcg gatgcggcgt gaacgcctta ttcgacctac acgccacaca tttccctgcc
     7801 aaatttgcgc cccatatctc actatccatt aacggatttg tgacagaaac cattgtcttt
     7861 ttttatattt gcttaaatct ttttttgcgt tacttatggc tggcgctggc attttccgct
     7921 gtgcgtttta gcgttcatgg tattaatcgc tcagatatcc accttttcct cagatttttt
     7981 tcactaaatg cataaaactt aatcaccaaa tgttgctaaa tattaagctg attgttctgg
     8041 tcatgttgga gtagcacaaa tttctctgct gcaaatagct gtttgaagtt tttttaatct
     8101 ttgtttgtgg attctcaccg ttggtgtaaa tcccggttgg ctgtattgac gttttcacat
     8161 tctgttgaca gattgtaggt cacgaggggc attttatgga ggatccgcac tgttacactg
     8221 atgttaatta gtacggcatc cccacctcat aacgttgacc cgaccgggca aaaaacaaaa
     8281 aaggtcaggc agcgacaacc cactgcaaag ggttaaaaca acaaacatca caattggagc
     8341 agaataatgc gtatttcctt gaaaaagtca gggatgctga agcttggtct cagcctggtg
     8401 gctatgaccg tcgcagcaag tgttcaggct aaaactctgg tttattgctc agaaggatct
     8461 ccggaagggt ttaacccgca gctgtttacc tccggcacca cctatgacgc ctcttccgta
     8521 ccgctttata accgcctggt tgaatttaaa atcggcacca ctgaagtgat cccgggcctc
     8581 gctgaaaagt gggaagtcag cgaagacggt aaaacctata ccttccatct gcgtaaaggt
     8641 gtgaagtggc acgacaataa agaattcaaa ccgacgcgcg aactgaacgc cgatgatgtg
     8701 gtgttctcgt ttgatcgtca gaaaaacgcg caaaacccgt accataaagt ttctggcggc
     8761 agctacgaat acttcgaagg catgggcttg ccggagctga tcagcgaagt gaaaaaggtg
     8821 gacgacaaca ccgttcagtt tgtgctgact cgcccggaag cgccgttcct cgctgacctg
     8881 gcaatggact tcgcctctat tctgtcaaaa gaatatgctg acgcgatgat gaaagccggt
     8941 acaccggaaa aactggacct caacccaatc ggcaccggtc cgttccagtt acagcagtac
     9001 caaaaagact cccgtatccg ctacaaagcg tttgatggct actggggcac caaaccgcag
     9061 atcgatacgc tggtcttctc tattactcct gacgcttccg tgcgttatgc gaaattgcag
     9121 aagaatgaat gccaggtgat gccgtacccg aacccggcag acatcgctcg catgaagcag
     9181 gataaatcca tcaacctgat ggaaatgccg gggctgaacg tcggctacct ctcctataac
     9241 gtgcagaaaa aaccgctgga tgacgtgaaa gttcgccagg cactgaccta cgcggtgaac
     9301 aaagacgcga tcatcaaagc ggtttatcag ggcgcgggtg tatcagcgaa aaacctgatc
     9361 ccgccaacca tgtggggcta taacgacgac gttcaggatt acacctacga tcctgaaaaa
     9421 gcgaaagcct tgctgaaaga agcgggtctg gaaaaaggtt tctccatcga cctgtgggcg
     9481 atgccggtac agcgcccata taacccgaac gctcgccgca tggcggagat gattcaggct
     9541 gactgggcga aagtcggcgt gcaggccaaa attgtcacct acgaatgggg tgagtacctc
     9601 aagcgtgcga aagatggcga gcaccagacg gtaatgatgg gctggactgg cgataacggg
     9661 gatccggata acttcttcgc caccctgttc agctgcgccg cctctgaaca aggctccaac
     9721 tactcaaaat ggtgctacaa accgtttgaa gatctgattc aaccggcgcg tgctaccgac
     9781 gaccataaca aacgcgttga actgtacaag caagcgcagg tggtgatgca cgatcaggct
     9841 ccggcactga tcatcgctca ctccaccgtg tttgaaccgg tacgcaaaga agttaaaggc
     9901 tatgtggttg atccattagg caaacatcac ttcgaaaacg tctctatcga ataattaaaa
     9961 gccatacccg gtggcgctgt gcctctgcgt gcagcgccat ccggcagcaa tacttcattc
    10021 ccagcccgac ctgggcggga attgatttgt gagcaataca gacacgcagt tccaggctgc
    10081 gggtcactat agagaatccg ggttatgttg cagtttattc tccgacgttt gggactcgtc
    10141 atccccacgt ttatcggtat tacccttctc acatttgcct ttgtccacat gatcccgggc
    10201 gatccggtga tgatcatggc gggcgaacgt gggatctccc cagagcgtca cgcgcagctg
    10261 ctggctgaac tcggcttaga taaaccgatg tggcagcagt atctccatta catttggggc
    10321 gttatgcatg gcgatctagg catttcaatg aaaagccgca tcccggtttg ggaagagttc
    10381 gtgccgcgcc tccaggccac gctggaactt ggcgtctgcg cgatgatttt tgcgactgcg
    10441 gtcggtattc cggtcggcgt gctggctgcg gttaaacgcg gttccatttt cgatcacaca
    10501 gcggttggcc tggcgctgac cggctactcg atgccgatct tctggtgggg catgatgctg
    10561 atcatgctgg tttcggtgca ctggaacctg acgcccgtct ccggtcgcgt gagcgatatg
    10621 gtgttcctcg atgactccaa tccgttaacc ggttttatgc taatcgacac cgccatctgg
    10681 ggtgaagacg gcaactttat cgatgccgtc gcccatatga tcttgcctgc cattgtgctg
    10741 ggtactattc cgctggcggt cattgtgcgt atgacacgct cctcgatgct ggaagtgctg
    10801 ggcgaggatt acatccgcac cgcgcgcgcc aaagggctaa cccgcatgcg ggtgattatc
    10861 gtccatgcgc tgcgtaacgc gatgctgccg gtggtgaccg ttatcggcct gcaggtggga
    10921 acattgctgg cgggggcgat tctgaccgaa accatcttct cgtggcccgg tctgggacgc
    10981 tggttgattg acgcactgca acgccgcgac tatccggtag tgcagggcgg cgtattgctg
    11041 gtggcgacga tgattatcct cgtcaacttg ctggtcgatc tgctgtacgg cgtggtgaac
    11101 ccgcgtattc gtcataagaa gtaaggggac atcatgtcac aggttactga aaataaagtg
    11161 attagcgcac cggtgccgat gaccccgtta caggagttct ggcactattt taaacgcaac
    11221 aaaggcgcgg tcgtcgggct ggtttacgtc gtcatcgtgc tgttcatcgc gatctttgcc
    11281 aactggattg caccctataa cccggcggaa cagttccgcg atgcactgct cgccccgcca
    11341 gcctggcagg aaggcggcag catggcgcac ttgctgggca ccgatgacgt aggccgtgat
    11401 gtgctgtcgc gcctgatgta cggtgcgcgc ctgtcgctgc tggttggctg tctggtagtt
    11461 gtgttatcgc tgattatggg cgttattctc ggcctgatcg ccggttactt tggcggcctg
    11521 gtcgataaca tcattatgcg cgtggtcgat atcatgctgg cgctgccaag tctgctgctg
    11581 gcgctggtgc tggtggcaat tttcggcccg tcaattggta acgccgcgct ggcactgacc
    11641 ttcgttgcct tgccgcacta tgtgcgctta acccgcgccg ccgtgctggt ggaagttaac
    11701 cgcgattacg tcaccgcgtc tcgcgtggcg ggtgccgggg cgatgcgtca gatgtttatt
    11761 aacatcttcc cgaactgcct tgcgccgctg attgttcagg cgtcgctcgg tttctctaac
    11821 gccattctcg atatggctgc tcttggtttc ctcggcatgg gggcacagcc gccaacgcct
    11881 gagtggggca ccatgctctc cgacgtgttg cagttcgcgc aaagcgcctg gtgggtcgtg
    11941 accttcccgg gtctggcgat cctgctgacg gtgctggcat ttaacctgat gggtgacggt
    12001 ctgcgtgacg cgctcgatcc caaactgaag cagtaagagg ttcgagatgg cgttattaaa
    12061 tgtagataaa ttatcggtgc atttcggcga cgaaagcgcg ccgttccgcg ccgtagaccg
    12121 catcagctac agcgtaaaac agggcgaagt ggtcgggatt gtgggtgagt ccggctccgg
    12181 taagtcggtc agttcactgg cgattatggg gctgattgat tatccgggcc gcgtaatggc
    12241 agaaaaactg gagtttaacg gccaggattt gcagcgtatc tcagaaaaag agcgccgcaa
    12301 cctggtgggt gccgaagtgg cgatgatctt ccaggacccg atgaccagcc ttaacccgtg
    12361 ctacaccgtg ggtttccaga ttatggaagc gattaaggtg catcagggcg gcaacaaaag
    12421 tacccgccgt cagcgagcga ttgacctgct gaatcaggtc ggtattcccg atccggcatc
    12481 gcgtctggat gtttacccgc atcagctttc cggcggcatg agccagcgcg tgatgatcgc
    12541 catggcgatt gcctgtcggc caaaactgct gattgccgat gaaccgacca ccgcgctgga
    12601 cgtgaccatt caggcgcaaa tcatcgaact actgctggag ctacagcaga aagagaacat
    12661 ggcgctggtg ttaattaccc atgacctggc gctggtggcg gaagcggcac ataaaatcat
    12721 cgtgatgtat gcaggccagg tggtggaaac cggtgatgcg cacgccatct tccatgcgcc
    12781 gcgtcacccg tatactcagg cattgctgcg tgcgctgcca gaatttgctc aggacaaaga
    12841 acgtctggcg tcgttgccag gtgtcgttcc cggtaagtac gaccgcccga acggctgcct
    12901 gcttaacccg cgctgcccct atgccactga cagatgtcgc gctgaagaac cggcgctgaa
    12961 tatgctcgct gatgggcgtc agtccaaatg ccattaccca cttgatgatg ccgggaggcc
    13021 gacactatga gtacgcaaga ggccacctcg caacaaccgc tgttgcaggc tatcgacctg
    13081 aaaaaacatt atccggtgaa gaaaggcatg ttcgcgccgg aacgtctggt taaagcgctg
    13141 gatggcgttt cgtttaacct tgaacgtggc aaaacgctgg cagtagtggg cgaatctggc
    13201 tgcggtaaat cgaccctcgg tcggttgctg acgatgattg aaatgcccac cggtggcgag
    13261 ctgtattacc aggggcagga tctgcttaag cacgatccgc aggcgcagaa gctgcgtcgg
    13321 cagaaaatcc agatcgtctt ccagaaccct tacggttcgc tgaatccgcg taaaaaagtt
    13381 gggcaaattc tcgaagagcc gctgcttatt aacaccagct taagcaaaga acagcgtcgg
    13441 gaaaaagccc tgtcgatgat ggcgaaagtc ggcctgaaaa ccgagcacta tgaccgctat
    13501 ccgcatatgt tctccggcgg acaacgtcag cgtatcgcta ttgcccgtgg tctgatgctc
    13561 gacccggatg tggtgattgc cgatgagccg gtttctgcgc tggacgtctc agtgcgtgcg
    13621 caagtgctga atctgatgat ggatttacag caggaactgg ggctttctta tgtctttatc
    13681 tcccacgacc tgtcggtggt ggagcacatt gccgatgaag tgatggtgat gtacctgggc
    13741 cgctgcgtgg agaagggaac gaaagaccaa atcttcaata acccgcgcca tccgtacact
    13801 caggcgctac tctccgcgac gccgcgcctg aacccggacg atcgccgcga acgcatcaaa
    13861 ctcaccggtg aactgccaag cccgctgaat ccaccgccgg gttgcgcctt caacgcccgc
    13921 tgtcgtcggc gcttcggccc ctgcacccag ttgcagccgc agctaaaaga ctacggcggt
    13981 caactggtag cttgttttgc tgttgatcag gatgaaaatc cgcagcgtta actcacaaaa
    14041 ccggggccat gcgctccggt ttactcaatc agcttcaaca gtggcgacaa gcatagcaaa
    14101 atgccgtaaa gcagaattag ccaggcttta aatccgcgaa gtttttccag ttgtgcgact
    14161 ttatagatca ggaaaaacgg aatgaggcac gacacaatgc catataacgg gcttcccaac
    14221 tgaaagaaca ccagcaccga tacacgaaac gaaacccaaa tcgtcaatgt aatgacgata
    14281 aaagcgcaga tcgccagagt cagcatgcgt gagttaattt tcttggtatc aataattcgg
    14341 cttaacagat tgagaataat gcctttaata gcctcgtgga aaccgagata aatgccaaag
    14401 aatgcggtca gtacggcaaa gatattcagc accgtagagg tgatatgaat gatatgtcca
    14461 gggatcacct gcgcggccag cgccagtgcc gagatatttt gttcaaaggc agaaacggct
    14521 tcttcgtggc taattgagaa ggtaaacgaa aaggcaaaaa acaggatcac cgcgatgagc
    14581 gtgatataac taattcggtg ggtacgcagc gcgagccggg ttgccagtac tttatccgct
    14641 tcccgtttac gataggcaat attcattggg ttaagtacct gaataaatac tgcagaaaag
    14701 aagcaaaatg gaatggtaag caagacatcg cggaaaaaga cggaggcttg cgggaaggcg
    14761 gttatattgg cgaaattcca gtgcgggatc atcgcaaaac cgaacacgac aataatccct
    14821 actttgacca ccaccattgg cccggaaatc ttaaacagta atcgttcacc accagacgca
    14881 atcgccacca gtacggcgaa aatagcgact ttataaagta gagattgtga aagatcggca
    14941 tcggttaaac cgaaggtttt caggtacgag gcgctgtcga aaaccacgga gagagagtag
    15001 ataaaaatcc cgtggataat catcaaaaag tagataaccc cgaggaaaat tccccagttc
    15061 ttccccaggt aatgactgat aatatcggtg tagtcattac aggaatcgct ttcagaaagg
    15121 gtttttaaat aaatgtcctg cactacccag gtggcaggat aagcaatgat cgctgcggta
    15181 ataaataccc aaattccctt caagccaatt tgtactggca tcagcacggt tccggcacca
    15241 atcgccatgc ctatgcataa taaaacccag ccgaagtcgt agcgtgtaaa cggcaatttc
    15301 tgattgtgtt tcgatagtgt gttgtgctgc ataaagtcct gtccagaaag aagtcggcct
    15361 cgcaaaaatg aataaaccca gtttctcttg cgcaacaggt ggagaagtcg aatacgcagt
    15421 atgtaaaaaa tgaggcaaca gattcactgg ggaggtcgcg aggaggcgca attctacaaa
    15481 attgcgcatt tcactaccag tctggagtca gcccggatat gaactgcaac tattgcaaat
    15541 aaagaaatta agctggaagg catcgcgcaa tgaggaagat tgccgggtgc tgacagtaaa
    15601 aagcaatcgt aaaatcagcg gcaagccgaa agggttccgg tgaggcgcaa tgttgcgggg
    15661 gctttatccc tggtggcatt ggttgctgga gagagaaaac ccccgcacgt tgcaggtatg
    15721 tacctgacaa cataacgggg gctaatcttg aacctagacc actcaagaat agccgcgaaa
    15781 cgttgtcatt acaacacagg cggctatatg acgctcgcag agctgggcat ggccttctgg
    15841 catgatttag cggctccggt cattgctggc attcttgcca gtatgatcgt gaactggctg
    15901 aacaagcgga agtagcgtgt catgtgggcg tcaggctgcc gtaatggcaa tttgcgcccg
    15961 gaccaggccg caggagtgaa actctgcggc ctttttcgta agaaaaatgc aacggttgca
    16021 agcggataat attctgcgga atgcgcagca cagagtgaag atgagacgga ttccggaaca
    16081 gaaaaacagt cgtagaatca gcgtcaagcc ggaaggattc cggtgaggcg caatgttgcg
    16141 ggggcttaat ccctggtggc attggttgct ggagagagaa aacccccgca cgttgcaggt
    16201 atgcacctga caacaccacg ggggccattc ttgcatccaa acaatacaag aatagccgcg
    16261 aaacgttgtc attacaatac aggcggctat atgacgctcg cagaactggg catggccttc
    16321 tggcatgatt tagcggctcc ggtcattgct ggtattcttg ccagtatgat cgtgaactgg
    16381 ctgaacaagc ggaagtaacg tgctatgcgg gcgtcaggct gccgtaatgg caatttgcgc
    16441 ccggatcagg ccgcaggggt gaaactctgc ggcctttttc gtaagaaaaa tgcaacggtt
    16501 gcaagcggat aatattctgc ggaatgcgca gcacagagtg aagacgtgac ggattccgga
    16561 acagaaaaac agtcgtagaa tcagcggcaa gccggaaggg ttccggtgag gcgcaatgtt
    16621 gcgggggctt tatccctggt ggcattggtt gctggagaga gaaaaccccc gcacgttgca
    16681 ggtatgcacc tgacaacatc acgggggcca ttcttgaacc tgaacaactc aagaatagcc
    16741 gcgaaacgtt gtcattacaa cacaggcggc tatatgacgc tcgcagaact gggcatggcc
    16801 ttctggcatg atttagcggc tccggtcatt gctggcattc ttgccagtat gatcgtgaac
    16861 tggctgaaca agcggaagta acgtgtcatg cgggcgtcag gctgccgtaa tggcaatttg
    16921 cgcccggacc aggccgcagg ggtgaaactc tgcggccttt ttcgtaagaa aaatgcaacg
    16981 gttgcaggcg gataatattc tgcggaatgc gcagcacaga gtgagtaagt gatggatttc
    17041 ggaacagaaa aacagtcgta gaatcagcgg caagccggaa aggttccggt gaggcgcaat
    17101 gttgcggggg ctttatccct ggtggcattg gttgctggag agagaaaacc cccgcacgtt
    17161 gcaggtatgc acctgacaac ataacggggg ctaatcttga acccaaacaa ctcaagaata
    17221 gccgcgaaac gttgtcatta caacacaggc ggctatatga cgctcgcaga gctgggcatg
    17281 gccttctggc atgatttagc ggctccggtc attgctggca ttcttgccag tatgatcgtg
    17341 aactggctga ataagcggaa gtaacgtgtc atgcgggcgt caggctgccg taatggcaat
    17401 ttgcgcccgg atcaggccgc agaggttaaa ctctgcggcc tttttcgtgc ttactgcggg
    17461 taaggcaccc agtcgccgcc gttcaggcga acgtacggtt tatcctggta ttgaataact
    17521 actgcatttg agttctcgga gaccggtgct gtttgtggca acccactggt gagttttttc
    17581 cagtcaacat tgtcttcggt gaaaatcttg ccatcaagaa cgcgaaccac cagatcggag
    17641 atagccagga agctgctcgg ttggtcaatg acaatcggtg ccccctgatg tggtgccttc
    17701 atgccgaaga atttcacccc aacggggacg tctgtgatag acgggctagg gatatcacgt
    17761 aggccagata cctgcattct gtcgcccttc agcgcgccgc cgtgttccgg caccacgacc
    17821 accatcactt tacgacccga tttctccagt tcagtaaaga aggcgtccag ttcatcaaag
    17881 aatttctgcg cccgcgcttt gtaatccgct gttttgctga cccccggata atggttgccg
    17941 tcatgcagtg gaagcgtgtt gtagaacgtg gcactacggc tgtttttatc tttttcggta
    18001 acgtccagcc agcggttaag cacggcggta tcgtcataaa ccggcgaacc atcaaagccc
    18061 agcaaaataa ccggcagatt tgtttgatcc atcaattcag tctgcatgcc gccattttcg
    18121 cgaacttctt tcaaaaaacc gccgaactgg ccgttatgcc ccatcatcag gtgctgggta
    18181 aagcccagtt tcgaaaggtt atcaaacaga tagcagtcgt tatttgccgg ttgatacaga
    18241 ttagtgtgcg aagtctgccc gcagctggcg cgcagtaaac ggatcgccgc cgggccactg
    18301 taggaggtgg cggagttaaa gttcttgaac tcaatatcga aatgcgacca cagtggatgc
    18361 gacatcaacc cggcggcttc tatatccgac caggaaagcg aacagatgtt aatcaccagt
    18421 agttcaaatg gctgagcatc agcgggcagc gaagacggga aggtcgattt acgtttcgcc
    18481 tccgcgttat agaaattatt cagccaggcg ttaaggttcg ccgttgttgg cggtgcagtt
    18541 tgtgcgggca tatcacccac taccggtgcg ccacccgtcg ccgcaacggt tgccgctgcg
    18601 ttaccacccg tcgttgttac agtggtcgtc ggttgtccgg ctggccacaa ggagaaactt
    18661 ggtcccgcca gggtaagtac gttcagccat agcagtatgg caaccacaaa aacggtaatg
    18721 cgaatccatt gtgacaggaa taaccaggcc actaataaaa caaaaatggc cccaatcatc
    18781 tgccagttaa taaagcgtgt gacaaggtcg attaaataat cggtactgaa ccccgccacc
    18841 tgcgaaccct ggctcattat gctttccggg ccaggcaacc aggtgtcatg ccagaacaaa
    18901 gcaaagccga tcggcagggc aatccagtgg cgcaagcgat gcaggctgta gcgcggaatg
    18961 ggcatcagca gaaacgcggc aaacaccaaa ttgaggagcg gatggaagtt aagatatccc
    19021 gcccacaaca ggccgaactt aaccagaaaa tagaagttcc agccggaaag gccgcgccag
    19081 tattgccaga gggaagaagg catggcggta ttttgcgtaa attgagtcat tttttggttg
    19141 ccctggcttt ttccgtgcgg cgtaacgtcc cggccggttt aacataacga ggtttagcaa
    19201 agaacaaacg taaggtgtcg cgaatgcgtc gcaaagagtg ccgcgccaga tagcccagcg
    19261 ggaaaaatat cagtgcgcaa acgacaataa tttcaatgat atcgctgatg gtcatcatga
    19321 tgagcgctcc acagcatcat ctaacagtcg catgggttct ggtattcgtc gccagtggcg
    19381 accatcgtgc tcggcattga tgaccggttt agaactttgc gttaaaggca gcggcatgcc
    19441 ccattgttct ggggcaagca agcgcatctg caccagctcg gcactgattt gatcatcttc
    19501 aaaccagacc atacggtttg agaaaatgtc gccagtaggc aatgggaaaa tatgattcaa
    19561 cgcggtatcc agatcgttaa tccgacagaa tgagagaaac agcaccagcc gattaccgcc
    19621 aatggtcatg atatcgccgg tacggttagg gcgacacagc gtcagggctt gttcaacgcg
    19681 gatacccggt accggacgta gggcaaccag aacgccttta ccgtgggcag gtaatagagg
    19741 gttattcatc atgttgttga cggcattaca gaacacatcc cacttctgga aaccacgcag
    19801 tttgagcggc tgggtcattg acagcaaggt agtgatatct tccggcacat agcgactaaa
    19861 cttctgccct tgcacgcttt cgatcatcgt cagacaacgg gagagtggcg cattccacgg
    19921 aataaccata tttgcaccgc aggccaataa caaacgttca tcggtggcgc gcaggctagc
    19981 ggtattttcc cgcacgagga ttttcatcgc actaccgcgc tggcgacgca gggtatgaat
    20041 gctgcgggcc agtggctcga tttgcgcatt ttgctgtaaa gaaaagacca ccgtcgccgc
    20101 ctgagcggta cgggcttcat tgaacaggac ttcgttattg ttgaacagtt gccagtgttc
    20161 cgatagcggc ggcgcacctt ccagtacagc aacattactc agaatgcgtt tttcgtcgct
    20221 gcgtggttgg atctccgcct cttcgctttg aactaatgtc cagataccat tttgttgctg
    20281 aacgctaagc tgctgacggg cgctgacccc tttttcgttg caccagaagg caatatccag
    20341 caaatgttgg tcaccctgaa aacgcaaact ggcaagacca aaaagtgaac ggtattcctc
    20401 aagcaacaat gaaaattgtt tatcgttatt atttccggga ttaattacca aaagcgaaca
    20461 atggtttaac ctgctccatt tattcatttt atccaaccat gagcgaagcc gctcggcagg
    20521 aatgttttgc catgcgttat ttgcgcaaac aagaataaaa aggtaattat ggggatcaat
    20581 agaacactgc aaatcacggg tcaaatagta tagacctttt tcatgattta gcattgaaaa
    20641 taattttatt ttttccggcc cttgagaatc atctaattga aagattttcg ccggatcgct
    20701 gtccatgcta atgaccgcga cgtgtgcggt ttcagcctgg gatgcaattg tttgattcgc
    20761 cagactgata gcatcttcat ggcgatcgac gttaaaccac cagacgccgc ctgctggcat
    20821 atgtcgcagc tcatcccata atgatgagat accgatagag aatacagggt ccacaatgtc
    20881 cctcatgtca ccattttgtc tgattatcag tttactagcg aaagctaaga aataaaccta
    20941 ccattgaaat taaaacttat cagatttagc atgtgaatca atttagaagg tcacaagacg
    21001 tgtgatttta gtcttttcgt ttttaacttt ttctgttgcg taacggaaag tcaaaaagtg
    21061 agcaaattcc cgtctcgccg taaacaataa accggcgaaa tgctcacggt tagaattgtc
    21121 tcatgaacgg tacggttatt tcatagggat caagcaaaat gaataacaat gaaccagata
    21181 ctctgcctga tcccgcgata ggctatatct tccagaatga tattgtggcg ttaaagcagg
    21241 cattttcact gcctgatatt gattatgccg atatttccca acgcgaacag ttggccgcgg
    21301 cattaaaacg ctggccgttg ctggcagagt ttgcgcaaca aaagtagggg attggtgaat
    21361 gg
//
LOCUS       DAZGVQ010000086        21230 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000086 DAZGVQ010000000
VERSION     DAZGVQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.087"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            324..824
                     /gene="tssB"
                     /locus_tag="ACHLAG_003072"
     CDS             324..824
                     /gene="tssB"
                     /locus_tag="ACHLAG_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFP0656257.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            859..1083
                     /locus_tag="ACHLAG_003073"
     CDS             859..1083
                     /locus_tag="ACHLAG_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656258.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1134..2609
                     /gene="tssC"
                     /locus_tag="ACHLAG_003074"
     CDS             1134..2609
                     /gene="tssC"
                     /locus_tag="ACHLAG_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP0656259.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2616..3029
                     /gene="tssE"
                     /locus_tag="ACHLAG_003075"
     CDS             2616..3029
                     /gene="tssE"
                     /locus_tag="ACHLAG_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP0656260.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            3033..4883
                     /gene="tssF"
                     /locus_tag="ACHLAG_003076"
     CDS             3033..4883
                     /gene="tssF"
                     /locus_tag="ACHLAG_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP0656261.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDNNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4847..5929
                     /gene="tssG"
                     /locus_tag="ACHLAG_003077"
     CDS             4847..5929
                     /gene="tssG"
                     /locus_tag="ACHLAG_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP0656262.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5954..7234
                     /gene="tagH"
                     /locus_tag="ACHLAG_003078"
     CDS             5954..7234
                     /gene="tagH"
                     /locus_tag="ACHLAG_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFP0656263.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPERHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGTPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7231..7755
                     /gene="tssJ"
                     /locus_tag="ACHLAG_003079"
     CDS             7231..7755
                     /gene="tssJ"
                     /locus_tag="ACHLAG_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP0656264.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7758..9089
                     /gene="tssK"
                     /locus_tag="ACHLAG_003080"
     CDS             7758..9089
                     /gene="tssK"
                     /locus_tag="ACHLAG_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP0656265.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQHFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDTRTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHHARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9094..9855
                     /gene="icmH"
                     /locus_tag="ACHLAG_003081"
     CDS             9094..9855
                     /gene="icmH"
                     /locus_tag="ACHLAG_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFP0656266.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9864..12629
                     /gene="tssH"
                     /locus_tag="ACHLAG_003082"
                     /gene_synonym="clpV"
     CDS             9864..12629
                     /gene="tssH"
                     /locus_tag="ACHLAG_003082"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFP0656267.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTMTDASDTLLARYAKNMTADARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMTLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESEQDNTGAVPADEADREQPEETAETVSPVQRLAHLTAELD
                     ALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLPKWLGDTIKGQDLA
                     IASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQYLTTINM
                     SEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDVLNLFYQ
                     AFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHADDPETMQEVLYPVLADFFKPA
                     LLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVIIEPEVTDEIMSRVTRA
                     ENGARMLESVIDGNMLPPLSLLLLQKMAANTAVARIRLSAVDGAFTADVEDAQNDESV
                     TKDETVL"
     gene            12626..13369
                     /gene="vasI"
                     /locus_tag="ACHLAG_003083"
     CDS             12626..13369
                     /gene="vasI"
                     /locus_tag="ACHLAG_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFP0656268.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKHRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13374..14786
                     /gene="tssA"
                     /locus_tag="ACHLAG_003084"
     CDS             13374..14786
                     /gene="tssA"
                     /locus_tag="ACHLAG_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFP0656269.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNADTAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14894..18328
                     /gene="tssM"
                     /locus_tag="ACHLAG_003085"
     CDS             14894..18328
                     /gene="tssM"
                     /locus_tag="ACHLAG_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFP0656270.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALMWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QALRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKELNAAPPESEEKLAVLRVMRMLEDKSGRNNQ
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KEREEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18339..19788
                     /locus_tag="ACHLAG_003086"
                     /pseudo
     CDS             18339..19788
                     /locus_tag="ACHLAG_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19715..20197
                     /locus_tag="ACHLAG_003087"
     CDS             19715..20197
                     /locus_tag="ACHLAG_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP0656271.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            20241..21155
                     /locus_tag="ACHLAG_003088"
     CDS             20241..21155
                     /locus_tag="ACHLAG_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656272.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLTSFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
     gene            21165..>21230
                     /locus_tag="ACHLAG_003089"
     CDS             21165..>21230
                     /locus_tag="ACHLAG_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656273.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFP"
BASE COUNT         5101 a         5710 c         5782 g         4637 t
ORIGIN      
        1 agcaatcatt agattagctt atgtttaatg aaattattat ataattcaat aagttacata
       61 aaaaatccat atttaaatta ttgccataat acacctgacg cgaattgcac aggttcacac
      121 aatctattta acgcttgcag aatacccctc tttacgatcg ataaaaagtc cttttcttat
      181 ttattttgaa gcgtaaaatg cggggtgatg aaggagtggt gattttattt tcaccgccat
      241 tagggcggca ttgccgatat ttttttgcgg tacaaatatg ccgtgttcat ggcctgtacg
      301 agataacatg acggatcact tttatgagca aaaaatttga aggcagcgtc gcgcctcgcg
      361 agcgtattaa tatatcgtat gtcccaaaaa cagatggcca gacggctgaa gtggaacttc
      421 cgcttaacat gctcgttgtc ggcgataccg gaaatacgca ggaaacatcc ccactggatg
      481 aacgtcaggc agtttccgtt aacaagcaca atttcggtgc agtgatggct gaagcagcca
      541 ttggcctcaa ttttactgtc cccgccacgc tgaagggaag cacaacagac gatgaactga
      601 atgtggcgct gaatatcaaa tcactggatg atttttcacc agatagcgtg gcccggcagg
      661 ttccggaagt gaataaattg ctggaactgc gtgaagcact gactgcactg aaggggccga
      721 tgggtaacct tccggcattc cgtacccagc tccaggcatt gctggaaaat gaagagtccc
      781 gcgagcagtt gctcaaagaa atcggtctgg tgagcaataa ataaccacca ctatttcatt
      841 aaacagggtt atccaggcat gacggcacac aaaaatatct ccgggacata ccacctctct
      901 gttgcagaca ttctgcaggt ggtttaccag gtatgtttca gcccgtccgt tgaaatcaac
      961 caggatggtg tggctgcgct gattacaaca ctggatcgcc gtatcagtga tctattggat
     1021 gagattatcc atttttgtga gtttcagcag agcgcgtcgc actggcagcg cgtacttcac
     1081 tgacggcgaa aagccggttt cagaaacaca gctaaaacag aaggattttc agtatgtctt
     1141 tacaggaaga ggaactcgta tccagtcatg ccgggcagcc tgagcaggca tcgtcgctgc
     1201 ttgatcaaat catggcacag acccgtattc agcccggttc ggaaggttat gacgttgccc
     1261 gccagggtgt gacagccttt attgccagca tcctgcaatc cactgcatct gccgaaccgg
     1321 tcaataaact ggccgtggac agcatgattg cagatattga tgaacgcatc agccgccaga
     1381 tggatgtcat catccatgcg cctgcctttc agcaggtcga gtcgttctgg cgctcactga
     1441 aaaccatggt ggatcgcgtt gatttccgtg aaaacatcaa ggtcaacgtg cttcatgtca
     1501 ccaagcagga acttctggaa gactttgagt ttgcgccaga aattattcag tccggatttt
     1561 ataagcacgt ttattcgtcc ggtttcggac agtttggcgg tgaacccatc gctgccgtgc
     1621 tgggcgctta tgagttcaaa aataccgcgc ctgacatgaa actgctgcaa tacgtcagtg
     1681 ccgtgggcgc aatggcgcac gcaccgttcc tgtcctccgt ttccccggag tttatggggc
     1741 tgaactcgtg gaccgaactg cctaatatca aagatcttta tgccatcttt gaaggtccgg
     1801 cctacaccaa atggcgcgcc ctgcgtgact cggaagattc gcgctattta gggcttacgg
     1861 caccccgctt cctgcttcgc cagccctatt caccaacaga taaccctgtt aagaacttta
     1921 actattacga agatgtcagc cagaaccacg aagattatct gtggggtaat acggcctgga
     1981 tgctggcatg caatatcgca gacagttttg ccaaataccg ctggtgtcca aacattatcg
     2041 gtccgcaaag cggcggcgca gtgaaagatc tgccggtgca tctgttcgaa acgatggggc
     2101 agattcaggc caaaatccca accgaggtgc tggtcaccga tcgccgcgaa tttgaactgg
     2161 ctgaagaggg ttttatcacc cttaccatgc gtaaagactc tgataatgca gcctttttct
     2221 ctgcaaactc ggtacaaaaa ccgaagcact ttccgggaaa agatgcagaa accaactata
     2281 agctgggtac gcagcttcct tatctcttca tcatcaatcg gttagcgcac tacatcaagg
     2341 tgttgcagcg tgaacaactg gggtcatgga aagaaaggag cgacttagaa agagagctta
     2401 atacctggat ccgtcagtat gttgccgacc aggaaaaccc gcctgcagac gtgcgcagcc
     2461 gcaaacctct gcgcgctgcc aaagttgagg ttatggatgt agaaggcgaa cccggctggt
     2521 accaggttgc gctaagcgtg aggcctcatt ttaaatttat gggggcaaat tttgagcttt
     2581 ccctggttgg ccggttagac agggagtaac acaccatgat accggatcgg gaaagaacca
     2641 caggttctct ttttgagcgt atggaagcct cttcggccag aaaccggcag ggaggcagta
     2701 ttcattcgct caggcaatcc attcgccaga acctacgaaa cattcttaat acccggtccg
     2761 gtagttgccg gggtgcgcct gagctgggca ttgatgaacc ggaaggggct gaaaacttcc
     2821 gcgaatccat gagtcgggcc atcgagcaat gtatagaacg ctatgaaccc cggatctcac
     2881 acgctgaagt gcaggcagtg gtttcatccg cctccagccc gctggatatg acgtttcata
     2941 ttacagcctg ggtgacattt aacgaaacgc atgaggtgct tgaatttgat atggcaccga
     3001 atggcagcca gcattaccgg gtggactgac aaatggaatt tgaagaacgc tatttccggg
     3061 aagaactcga ttacctgcgc cagcttagca agctgctggc aacggaaaaa ccccatctgg
     3121 cccgcttcct ggccgaaaaa gatgcggatc cggatattga acgcctgctg gaaggggtgg
     3181 cttttcttac cggcaatctc cgccagaaaa ttgaggatga attcccagaa ctgacgcacg
     3241 ggcttattaa gatgctatgg cctaattacc tgcgtccggt tccggcaatg acccttattg
     3301 aatatacgcc ggatatggat aagtcttctg taccggtgtt aatcccccgt aatgagcagt
     3361 ttacaaccaa cgccggggaa atcagagttg atgaagtgct gccctctgat gctaaaaagg
     3421 aggagccgcc tccctgtacc ttcactctct gccgggatat ctggctgctg cccgttcgcc
     3481 tggagcagat tgaaaaccgc agtacgaccc gtaatggtgt tatcaacatc accttttcgg
     3541 tcgcaccggg aacggacttc cgcacgctgg atctgaacaa acttcgcttc tggctcggca
     3601 atgacaacaa ctatacccgt gaccagcttt atttatggtt ctgcgaatac ttgcagggtg
     3661 ccgacctgac tgtgggtgaa cagcatattc gcctgcctga gtttatgcta aaagctgtcg
     3721 gttttgagcc gcaggatgcc atgctgccct ggccgaaaaa cgtccacagc ggctaccgga
     3781 tccttcagga gtatttctgt taccccgatg cgtttctctt ttttgatctt tgtggttgtc
     3841 cggctttgcc tgacggattg caggcggaat tctttaccct gcaactgcgt ttttcgcgcc
     3901 ctttgcccgt ggacatccgg ctgcgccgcg attccctgcg cctgtattgc gcacctgcca
     3961 ttaatttatt tatccaccat gcagaagcca tcacgctgga caaccggcgg gcagactatc
     4021 cgctggttcc cagccgccat tacccacaac attacgatgt attttccgtt aacagtgtgg
     4081 tgagccaggt ccaggatatg ttcaggaaaa aagatctggg gcgtcctgtt tcgacgcagg
     4141 ccgcgcgcca gtggccagcc tttgaaagtt tcagccatca gatggaatac agccggaagc
     4201 gggaagtggt gtactggcat caccggacca aaacatccct gttccatcgc ggctttgatc
     4261 atacccttgc ctttatacat gctgatggca gttatccgtc agacgaatct ctgctcagta
     4321 atgaagtggt ttcggtatcg ctgacctgta ccaaccgtga gcttccgtca caaattcgtt
     4381 ccggcgatat caccggcaca accggtaaaa atgcggctgt tgcttcattt cgcaacatta
     4441 cccgcccgac gcaaccactc tggccggtca ttgatggcag cctgcactgg tccctactct
     4501 ccgccatgaa cctgaattat ctgtcattac tggatacgga cgcgctgaag caggtcatcg
     4561 ccaactttga tcgccacgca atccatcatc cgcagacggc acggctgtca caacaaaagc
     4621 tggatgccat tgagcgtctg gagacccgcc ccgttgatcg cctgtttacg ggtattcccg
     4681 tccggggact ggcctccacg ctgtatctgc acccggagcc gtttgtctgt gaaggggaaa
     4741 tgtatctgct cggtacggtg ctttcgcatt ttctgtcgct gtacgccagc gttaactcat
     4801 tccacatgct gaccgttgtg aacacagaaa gccaggagac atggaaatgg acggaaagaa
     4861 tcgggcagca tcctcttatc tgagtccggg aaatcccccg gcagataaag agcagaatga
     4921 tccgctggca caggttttcc ataatgcctg ctcttacaat ttttttgcga tggcggagct
     4981 gttgcaccgc ctggcaaagg gtgaaaaggg gacgccagaa ttatccctgc gtgacgatcc
     5041 ggcacaggaa accctgcgtt tcagcgccga tgccagcctt gccttcccgt gcagtgatat
     5101 cagtgcactg aaacgggata cgtcaggggc attcaggatg accaccacct ttatggggtt
     5161 acaggggagc cagtcgcctc tccccggtta ttacctcgat cacctggcct ggaaagcggt
     5221 acatgaacaa agtccggtag gtgattttct ggatatgttc agccaccgcc tgacccagtt
     5281 tgtctggcat atctggcgta agtaccgcta tcacatcagt tttcgtaacg gcggtgtgga
     5341 tgcgttctcg cagcgcatgt actcccttgt cgggctgggc caccgccagc ttcgcgataa
     5401 gctggcgatt aatcacagca aaatgctggc gtattccggg atcctggcaa atccgggacg
     5461 atccccggaa ataatctgtg gcctggtgtc ccactgcttt gatttgtcag aagtgacgct
     5521 gcaaaactgg caacgtcgca aggtggatat tgaaccggat cagcaaaata gtctggggag
     5581 ctattctctt aaaaatggcg aaaaactggc gggaagatcg gtactgggca actttgttct
     5641 gggtacacga gtgcccgatc tcagcggaaa attccagctc agtattacca gcctgacccg
     5701 aaaacagttc ctctcttttc tgccgtccgg cgaaaacttc ctgccactga cgatgttcgt
     5761 gtccttcatc ctgcgcgatc agcttgcctg ggacttacat ctggggctgg cgccggagca
     5821 ggtgggcgca atgcgcctgg gcgataacaa aagtgcgctg ctgggctgga ccagcttcct
     5881 cggcacaccg gaagaacgac cctcagtcac tatcagggta cggtcgtaaa ttatgataat
     5941 cacggagcag aatatgccgg aagagaaact acagacgctt tcattgcagg tcattaacgg
     6001 cagtgagctg gaaagcggac gggcggcgcg ctgtctgttc acacagcagg gaaatgtcgg
     6061 ccatggcccc gaacgccact ggtcggtaca ggatcgtcag cagagcattc cggcccaggc
     6121 ttttaccgtt atcctgcacg atggcacatt ttgtctgcgc ccgcagacgg cacaactgtg
     6181 gctgaatcag gcaaaagtca cagcaacatc agacctaata cagttgcgcc agggcgatga
     6241 gatccagatc ggacggctga tggtgagggt tcatctgaac cggggagata ttccccatta
     6301 cgatgaggaa atggccactc ccgaaaccat cgttaccaat cgcgatatgc tcacggatac
     6361 cctgctatca acggagggta cgccacacta tccgggaatg actcaccggc accaacttgc
     6421 agacaccgtg gtaaacggtt tttctgccga tccactccag gcacttcagt ccgaaagcct
     6481 gattaccacg ggcgatccgc tttcaggcat tgcggctgtc cggccatcgg caccgctgtc
     6541 cgatccggca agtaatgggg ggatcaatac tccgtttatg gatctgccgc ccatttatgc
     6601 cagccccggc gatcgtaatg acgacgtctc tgcggcagaa atggcgcaac gccaccttgc
     6661 ggtcaccccc ttactgcgcg gtcttggcgg ctcgcttacc atgagcaatt ccgacgatgc
     6721 ggatgatttt ctggaggagg ccggacgaac gttacaggcc gcaataaaag gtctgctcga
     6781 tttgcagcag cagcgtaaca gcctctcaga caaacatttg cgcccgctgg aagataaccc
     6841 gctgcgcctg aacatggatt acgccaccgc gctcgacgta atgtttgccg aaggtaaaag
     6901 cccggtacat ttggcggctc ccgccgccgt cagtgaaagc ctgcgcaatg tccgccacca
     6961 cgaagaagct aaccgggcag cgattgtgga gtcgcttcgt gtcctgctgg atgctttctc
     7021 accacaaaat ctgctgcgcc gctttgtgca gtaccgccgc agccatgaac tgcgccagcc
     7081 gctggatgat gccggagcat ggcaaatgta cagccattat tacgaagaac tggcctccga
     7141 tcgccagcag gggtttgaga tgctgtttaa cgaggtctac gcccaggtct atgaccgggt
     7201 gcttcgtgaa aaacagcggg agccggaagc atgaacagga aggcgtttct ggcctgcgtg
     7261 ctgatgtgcg tcatattaac cggctgcgaa acggcgaaaa aaatcagtga ggtgatcaaa
     7321 aacccggata tccaggtcgg gagcctgaag gagcaacctt cggaaataac cgtcaccctg
     7381 ctgaccgaac cggacaccaa taccaatgcg gaaggtgaat cagccgccgt ggatgtgcaa
     7441 ctggtgtacc tgactgacga ctcaaaattg ctggcagccg actatgacca gatagccagt
     7501 acgccgctac cggatgtgct gggtaaaaac tatatcgacc accaggattt caacctgttg
     7561 ccggacacca tcaaaaccct gccgccggta aagctggatg agaaaacgca gtttattggt
     7621 gtcgtggcct atttctccga tgaccaggcc actgaatgga aacaaatcga gacggtggaa
     7681 ggaaccggcc accattaccg cctgctggtg catgtacgtc aaagcagcat cgaaatgaaa
     7741 aaagaggatg aataattatg gcaaccaccc gcaacaaggt gatgtggcag gaagggatgc
     7801 tgatgcgtcc acaccatttc cagcagcagc agcgttacaa cgactacctg gataaccagc
     7861 gtttccgggc catgaatgat ttatcctggg gatttaccga actcactctc aacaatgaac
     7921 tgctggcaca gggtaagatc atgattgaca gcgcgtcagg cacactgccc gacggcaccg
     7981 tcttttctat ccccgaccag gacgcactgc ccgatccgct gcacccgcaa cattttccgg
     8041 acgagagaag ccgcaatatc tacctcgctc tgccggtcgc cagtgatgtg agaaatgaaa
     8101 tcagcgacgg gcggcgaatc gggcgttacc ggctgaatta tgccgatgtc cgggatttgc
     8161 attcagaaga aggtgacacg cgaacactga cgctgggaca actgacgccg cgcattatga
     8221 gcggtgcaga agatatgagc gcctatatta cgctcccgct ttgccgtatc agcgatcgcc
     8281 atgccgacgg ctccctgacg ctggatgacg attttatccc ctcctgccag aatattcagg
     8341 tcagtaagaa actgcgtgtt tatctcaaag aggtacaggg ggccattggc ggacgggcaa
     8401 gcgatctggc aaaccgcatt ggctctccgg cgcagagcgg catcgcggat gtggcggaat
     8461 ttatgatgtt gcagttactt aaccgtaacc agacccggtt tacccatcac gcccgtcgat
     8521 cccagctcca cccggaagat ttctaccttg atcttgccgg gttgctgggt gaactgatga
     8581 cctttaccga gccgtcgcgc ctgccctgcc cgcttgatgt gtatgatcat catgacctga
     8641 ccaaaacatt taaaacgctg ttaccggaag tcaaacgggc gctgcatacc gtactgtcgc
     8701 caagagccgt caatctgccg ctgcatctgc gtgacggtat ctggcaggcc gatatccatg
     8761 acacagaact gctgcaatct gccacctttg tgctggccgt ggcggcaaac atgccggtcg
     8821 atcagatcca gcgccagttt atccagcagt cgaaaatttc ctcgccggaa aaaatccgca
     8881 atatggtcag tgtgcagatc cccggcattc cattgcgcgc actgatggtg gctccccgcc
     8941 agcttcctta ccattccggg ttcagctatt tcgaactcga caagagcgga caggcctgga
     9001 cagaaatggc tgccgccggg gccgttgcac tgcatgtatc cggcagtttc ccggatctga
     9061 acatgcaact gtgggcgata agagggtaag acgatggatg aggggtcact gtctctgcca
     9121 ccatttaccg gatacgacga aaaaagccag cgcaactacc atctcgcgct tcgtggcaac
     9181 agcctgaatc cgatgatcga cgcggccacg ccgctactcg gtatggtaat gcgcctttca
     9241 accatgaaca gccagacgat gccggagcat ctttttgctc aggtggtgac ggatgttcag
     9301 gccgtggagc aactgcttca ggagcagggc tacgaaccgg gcgtgattat ctcgtttcgc
     9361 tacatcctct gcacgtttat tgatgaagcg gcgctcggta acggctggtc aaacaagaac
     9421 gagtggatca agcagtcatt gctggtccat ttccacaacg aagcctgggg tggagagaaa
     9481 gttttcatcc tgctggagcg cctgatccgt gaacccaaac gttaccagga tctgctggaa
     9541 tttctctgga tctgtttttc tctgggattt cgcgggcgct acaaagtggc agcacaggat
     9601 cagggggaat ttgagcagat ctaccgccgt ctgtaccacg ttctgcataa actgcgcggc
     9661 gatgcccctt tcccgctgtt acaccaggac aaaaaaaccc agggcgggcg ttatcagctc
     9721 atcagtcgcc tgactgttaa acatattttt tgtggtggcg ttgtcgtgct ggcgttgttt
     9781 tacctgttct atctgttgcg gctggacagc cagacgcagg acatcctgca ccagctaaac
     9841 aaattacttc gataaggacg tttatgatcc agattgatct tcccacgctg gtaaaacggc
     9901 tgaacctgtt ctcccgccag gcgctggaga tggccgcctc tgaatgtatg agtcagcagg
     9961 cagcggaaat taccgtcagc catgtgctca ttcagatgct cgccatgcca cgcagtgacc
    10021 tgcgggttat tacccgccag ggcgatattg gcatggaaga gttgcgccag gcgctgacgg
    10081 tagagaacta cacaaccgcc cgttctgcgg acagctaccc ggcgttttcc ccgatgctgg
    10141 ttgagtggct gaaagagggc tggctgctgg cgtcggctga gatgcagcac agcgaactgc
    10201 gcggcggcgt gttgctgctg gccctgctgc attcgccgct gcgttatata ccgcctgctg
    10261 ccgcccggct gttgaccggc attaaccgtg accgtctgca acaggacttt gtgcagtgga
    10321 cacaggagtc ggcggaatca gtcgtgccgg atgcagacgg taaaggcgca ggcacaatga
    10381 cggacgcctc tgacaccctg cttgcccgct atgccaaaaa catgaccgca gacgcccgta
    10441 acggcaggct tgacccggta ctgtgccgcg accacgaaat cgacctgatg atcgacattc
    10501 tctgccgccg ccgtaaaaac aacccggtgg tggtgggcga agcgggcgtg ggcaaaagcg
    10561 cactgattga agggctggcg ctgcgcatcg tggcaggcca ggtgccggac aagctgaaaa
    10621 acaccgatat catgaccctt gacctgggcg cattgcaggc cggggcgtcg gtgaagggtg
    10681 aattcgaaaa acgtttcaaa gggctgatgg cggaggtcat ttcctccccg gtgccggtca
    10741 ttctgtttat cgacgaagca cataccctga ttggcgcggg caaccagcag ggcgggctgg
    10801 atatctccaa cctgctcaaa ccggcgctgg cgcgcggcga gctgaaaacc atcgccgcca
    10861 ccacctggag cgagtacaaa aaatacttcg aaaaagatgc cgccctgtcg cgccgcttcc
    10921 agttggtgaa ggtcagcgaa cccaacgctg ccgaagccac cattattctg cgcggtctgt
    10981 cggcggtcta tgaacagtct cacggcgtgc tgattgatga tgacgccttg caggccgctg
    11041 cgacattaag cgagcgttat ctctccgggc gtcagttacc ggacaaagcg attgatgtgc
    11101 tggataccgc ctgcgcccgt gtggccatca acctgtcgtc gccgccgaag caaatctcgg
    11161 cgctgaccac tctgagccac cagcaggagg cggaaattcg ccagcttgag cgcgagcttc
    11221 gcatcggact gcgtaccgac acatcacgga tgaccgaggt gctggtgcag tatgatgaaa
    11281 cgctgacggc gctggatgaa ctggaagcgg cctggcacca gcagcagacg ctggtccggg
    11341 agattattgc gctgcgccag cagttactgg gcgtggcaga ggatgatgcg gcgccgttgc
    11401 cggacgcaga taccgtggag gatacgcagc cagagtcaga gtcagaacag gataataccg
    11461 gtgccgtacc ggctgatgag gccgacagag aacagccgga agagaccgct gaaacagttt
    11521 ccccggtaca acgtctggca catctcactg ccgaactgga cgccctgcat aacgaccggt
    11581 tgctggtttc cccgcacgtc gataaaaaac agattgcggc ggtgattgcc gaatggaccg
    11641 gcgtgccgct taaccgcctg tcgcagaatg aaatgtcggt catcaccgac ctgccaaaat
    11701 ggctcggcga caccatcaaa ggccaggacc tggcgattgc cagcctgcac aaacacctgc
    11761 tgaccgcacg cgccgacctg cgccgtccgg gacgcccgct gggcgcgttc ctgctggctg
    11821 gccccagcgg cgtgggtaaa actgaaaccg tcctgcaact ggcagagctg ctctacggcg
    11881 gtcgccagta cctgaccacc atcaatatgt ccgagttcca ggagaaacat accgtctcgc
    11941 gactgattgg ttcgcctccg ggctacgttg gctacggtga aggcggcgta ctgaccgaag
    12001 cgattcgcca gaaaccctac tcggtagtac tgctcgatga agtggaaaaa gcgcacccgg
    12061 atgtgctcaa cctgttctac caggcgttcg ataagggcga aatggcagac ggtgaaggcc
    12121 gcctgattga ctgcaaaaat atcgtcttct tcctgacgtc caacctcggc taccaggtaa
    12181 tagtcgagca tgccgatgac ccggaaacca tgcaggaagt actgtatccg gtgctggccg
    12241 acttcttcaa acctgccctg ctggcgcgta tggaagtggt gccgtacctg ccgctgtcga
    12301 aagagacgct cgccaccatc attgccggga aactggcccg cctggataac gtgctgcgca
    12361 gccgctttgg cgcggaggtg attatagaac cggaagtgac ggacgaaatc atgagccgcg
    12421 tcacccgcgc ggaaaacggc gcgaggatgc tggaatcggt catcgacggc aatatgctgc
    12481 cgccgctctc cctgctgctg ttgcagaaaa tggcggcgaa tacggcggtt gcccggattc
    12541 ggttgtcggc agtggacggc gcatttacgg cagacgtgga agatgctcag aacgacgagt
    12601 ccgtcacaaa ggatgaaacg gttttatgag caggttgcgc gttttccggc agacaggatg
    12661 gtttatcgcg ggcctgatga cgggcttacc cgccactgcc gcgcccgcag aagcatcgtc
    12721 aatggccggt gtggcggtcg ccgtcgccac cacgaccccg ccggatgcga cagccacgtt
    12781 acaggccatg cagtcctgtc ggcgggagtc cgcggcgctg gagcggcttg actgctatga
    12841 ccgcctgctg gcaccgctgt ccccgtcagg gtttgacggg gcgctggtca aagccggttt
    12901 tgtgggcgaa gcctggaccc gcgccacgga acaggaaaaa caccgccagg gcaacaccac
    12961 agagctgctg gtgacgcagg tgccggggga gcgcccgacg gtggtgataa ccaccccggc
    13021 tatcggtcat gttccgccac gcccggtgct gatgttcagt tgcgtggaca atatcacccg
    13081 gatgcaggtg gcgctgatgc accctctgga tgtccatgat atcgccgtca ctctgaatgc
    13141 cgacaaccgg gcattgcgca gccactggtt tgtgcgcgag aacggcaccc tgctggagtc
    13201 cagccggggg ttatccggga ttgatgaaat taagcaactg ttcggcgcaa aaacgctgac
    13261 ggtggatacc ggcgcagaca atgccgccgg aaagctgacc tttaacattg acgggctggc
    13321 aagggcgatt gctcccctgc gcgatgcctg ccactgggcg ggagaataaa aatatggcaa
    13381 tggacttacg cgatccgaat gtctggatat cgcacctgct ggaaaacctg ccggaagaga
    13441 aactggcatc ggcgctgaaa gatgacaacc cgaactggga gtatatcgac ggcgaaatcg
    13501 tcaagctggg atcgctggcc cacagtcaac tggatatccc ggaactccag cgccgggggc
    13561 tggtcattct ggcttcggag agtaaggatt ttcgtctgct ggcgcatctg ctgagaaccc
    13621 tgcaacacgc cggtgatcct catctcgcat tgcgcctgct ggcgctgtat gtggagcatt
    13681 actgggcggt ggctgcgccg cagaatatgg cgcataaaaa acgctttgcc agccaggtca
    13741 ttaaacgctt tgaaacaggt attgaagttt tttcacagaa cgccgacaca gcgcagcgcg
    13801 atgcgctgtc aggggaactg gcaaaactgg cgcaatgctg gcagtcacat aacgccccgg
    13861 aactggcaca ggctactgat gatctttttg ccctgtacca gcgtgcgttt aatcgtgcgg
    13921 caccggctcc tgtccccact ccggcggcct ccggtagttc accacagacc accgccacgt
    13981 ctgaaagcgg cgtgacgcaa cccagtgctc cggctcccca aatcgtcatc gacagtcacg
    14041 acgacaaagc ctggcgcgac acgctgttaa aagtggcggc cattttatgt gaacgccagc
    14101 cggattcacc gcagggctac cgtctgcgcc gccatgcgct ttggcagaac atcaccagta
    14161 cgccccaggc ggaaagcgat ggccgtactc cactggctgc ggtctccgcc gatatggtgg
    14221 cagattacca tgctcagctc ggtagtgccg atatggcgct gtggcagcag gtggagaaaa
    14281 gcgtactgct ggctccttac tggctggatg gtcactgcct ttctgcacag acggcgctgc
    14341 gtctgggtta caaacaggtg gcagacgcca tccgcgatga ggtcatccgc ttccttgagc
    14401 gtctgcccca gcttaccggg ctgctgttta atgaccacac accgtttatc agtgagcaga
    14461 cgaaacaatg gctggcagct tcgcccgacg ccaaagttgc accggttgcg caaatcggtg
    14521 aggaatcgaa ggcagccaga gcctgttttg ctgagcaggg tctggaggcg gcgctgcgat
    14581 atctggacat gctacccgaa ggcgatcccc gcgatcagtt tcaccgccag taccttgccg
    14641 cacagttgac ggaagaggcg gggctggtac agcttgcgca gcagcagtac cggatgttgt
    14701 tcaggatggg attgcagatg atggtggcag actgggagcc gtccctgctt gagcagctag
    14761 aacaaaaatt cacggcagaa caataaaaga gaacaggagt ttttgtgttc agattaccaa
    14821 caccccgact actcagcgga caaaatcagc cctgcgaccg gcgatgcccc ggtttaaagt
    14881 ctctgctttc tggctgctga tactggcgtg gatttttctg ctggtgtgga tctggtggaa
    14941 aggcccgacg tggacgctgt atgaagaaca gtggctcaaa ccactggcga accgctggct
    15001 ggcaacggcg gcgtggggga ttattgccct gatgtggctt accgtccggg tgatgaagcg
    15061 cctgcaacag cttgaaaaaa tgcaaaagca acagcgcgag gaagccgttg atccgctcag
    15121 cgtggaactg aacgcccagc agcgttatct tgaccgctgg ctgctgcgcc tgcaacgcca
    15181 tctcgacaac cgccgtttcc tgtggcagtt gccgtggtat atggtcatcg gcccggcggg
    15241 cagtggcaaa acgacactgc tgcgcgaggg gtttccgtcc gacattattt atgccccgga
    15301 gggcgcacgc ggcgcagaac aacgcctgta cctcacgccc catgtcggta aacaggcggt
    15361 gatctttgat atcgacggca cactctgcgc tcccgctgat gcggatatcc tgcatcgccg
    15421 tctgtgggaa catgccctcg gctggctgaa agaaaagcgc gcgcgccagc cgctgaatgg
    15481 gattattctg acactcgatt tacccgatct gcttaccgca gacaaacgcc gccgcgagca
    15541 tctgttacag gcgctgcgca gccgcttgca ggatatacgc cagcatcttc actgccagtt
    15601 accggtttac gtggtactta cccggcttga tttattgcag ggttttgccg ccctgtttca
    15661 gtccctgaac agacaggatc gcgatgcgat tctgggcgtc acgttcaccc gccgtgccca
    15721 tgaaaatgat gactggcgaa cagagttgaa tgctttctgg cagacatggg tggatcgaat
    15781 gaatctggcg ttgccggatc tgatggtcgc tcagactcac acccgcgcgt ctttattcag
    15841 tttttcccgc cagatgcagg gaagccgtga accgctggtg tcactgcttg agggtctgct
    15901 tgatggcgaa aatatgaacg tgatgctgcg tggtgtctat ctcacctctt cgcttcagcg
    15961 tggacagatg gatgatatat tcacccagtc tgccgcccgc cagtaccggc tgggcaataa
    16021 cccactggcg tcctggcccc tggtggacac cgcgccttat ttcacccgca gcctgttccc
    16081 gcaggcatta ctcgcagagc ctaatctggc aacagagagc cgcgcctggc tgatacgttc
    16141 ccgtcgccgc ctgacggttt tctctgccac aggcggcgtg gcagcactgc tgctcatcac
    16201 cggctggcat cactattaca acggtaacta tcagtctggc atcaccgtgc ttaagcaggc
    16261 caaagccttt atggacgtgc cgcctccgca gggggaagat gactttggca acctgcaact
    16321 gccgctcctg aacccggtac gcgatgccac actggcctat ggcgactggg gcgaccgcag
    16381 ccgtctggcc gatatgggac tgtaccaggg acgacgtatc gggccttatg tggaacagac
    16441 ctatctgcaa ctgctggagc aacgttacct gccctcgctg tttaacgggc tggtcaaaga
    16501 gctgaacgcc gcgccgccgg agagtgaaga aaaactcgcg gtgctgcgcg tgatgcgaat
    16561 gctggaggac aaaagcggac gtaacaatca ggtggtgaag cagtatatgg caaaacgctg
    16621 gagcgaaaag ttccacggtc agcgcgatat ccaggcacaa ctgatgtccc atcttgacta
    16681 cgcgctggct catactgact ggcacgcaga gcgtcaggcg ggcgacggtg acgccatcag
    16741 ccgctggacg ccatatgaca agcccgtggt atcagcacag aaagaactga gcaaactgcc
    16801 tgtctaccag cgggtttacc agagcctgaa aacgcgggcg ctgggcgttc ttcctgccga
    16861 cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtgtttacat ctgccgatga
    16921 caacaaactg gttgttccac agtttcttac ccgttacggc ctgcaaagct attttgtaaa
    16981 acagcgcgat gaactggttg aactgacggc gatggattcc tgggtactta accttacccg
    17041 cagcgtgaaa tacagtgacg ccgaccgcgc ggaaatccag cgccagttga ccgagcagta
    17101 tatcagcgac tacaccgcca cctggcgggc cgggatggac aatctgaata tccgcaattt
    17161 tgagtccatc ggacaactga ccggggcgct ggagcaggtt atcagcggcg accagccttt
    17221 gcagcgggcg ctaaccgtgc tgcgtgacaa cacacagcca ggcgtctttt ctgaaaaact
    17281 ctctgccaaa gaacgggagg aagccctggc agagccggat taccagttac tcacccgcct
    17341 cgggcatgaa ttcgccccgg aaaacagtac cctggcagta cagaaagaca aagaaagcac
    17401 gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tacctgctgg caatccagaa
    17461 cgcgcctgta ccaggaaaat cggcgctgaa agccgtgcag ttacggcttg atcagaacag
    17521 cagcgatccg atattcgcca cccgccagat ggcaaaaacg ctgcctgctc cgctcaaccg
    17581 ctgggttggc aggctggctg accaggcctg gcatgtggtg atggtggagg ctgttcatta
    17641 tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac tggcaaataa
    17701 ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg aacgcttctt
    17761 taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt ttatcgataa
    17821 tgacctgagt ctggaggatg gcgataacaa cgtcattatt cgcgaagata ttattgcgca
    17881 actggaaact gcgcagaaaa tccgtgacat cttcttcagc aaacagaacg gtctgggaac
    17941 atcctttgcc gtggaaacgg tatcgctttc aggcaataaa cgccgcagtg tactgaacct
    18001 tgacggtcag ttagtcgatt acagccaggg ccgtaactat accgcccatc tggtctggcc
    18061 taacaacatg cgcgaaggca acgaaagtaa gctgacgctc atcggcacca gcggcaacgc
    18121 gccgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgcctgttcg gggccggaca
    18181 actgaccgga gtacaggatg gcaactttac cgtgcgcttt agcgtggacg gtggcgcgat
    18241 gacctaccgt gtgcataccg acacggaaga taacccgttc agcggtgggt tgttcagcca
    18301 gtttggtctg tcagacacac tgtactgagg aggcagggat gaatagtaac gtactgacac
    18361 aaactatcgt taccggcagt gacccgcgcg ggctgccgga attcagcgcc atccgcgagg
    18421 aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg gtggagtcgc
    18481 tggcgctggc gatttttaaa gccaacggtg tggatttaca caccgccacc tactatacgc
    18541 ttgcccggac acggacccag ggactggcgg gattctgcga aggtgcggaa ctgctggcgg
    18601 caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg cgtactgaaa
    18661 tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc tcctttgcgg
    18721 aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc tgcgataagc
    18781 tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat tttatgcaga
    18841 acacgcgtaa acggcttgaa ccccagctga agagtaacac tgagaacgcc gcacagacca
    18901 cggtcagaac gctgatttat gtcccggaaa cacaggcatc ttccacacca gaagcggtag
    18961 tgcctcccct gcccggccta cctgagatga aagtggaagt gcgcagtctg acagagaatc
    19021 ccccacaggc cagtgtgata aagcaaggca gtacggtaag agggtttatc gcagggatcg
    19081 cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg gtgcagcagc
    19141 aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg gcctcacctg
    19201 aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg gtacagccgc
    19261 tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa agcctgcaac
    19321 agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag agtagcccgc
    19381 agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat ctagtgatgc
    19441 agcgcgagaa agagggacta accctttcct atatcaaaaa tatcatctgg caggcggagc
    19501 ggggactggg gcaggaaaca cccgttgagt ctctgttgac gcagtaccat gatgcccgtg
    19561 cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc gaaggcgtgt
    19621 taagccgctg gctgctgctg aagaataacg tcatgccaga ggcggcaacc ggtaccacgg
    19681 ccgaaaaata attatttaat taaaggagat tatcatggca aatataagtt atttatcttt
    19741 gagtggagag acacaagggc taatttcagc aggttgctca acgcttgatt ctgtcggaaa
    19801 caaagcgcag ccagaacata aagatcagat catggtttat gcgttaatgc attccatctc
    19861 aagaagtcaa aatgtcaatc atcatgaatt aattatcacc aaaccggttg ataaatcctc
    19921 accattgctg gcaaaagcac ttagtgacaa tgaaaaaatg gcaatatgtg aatttatact
    19981 ctaccgaacc agtaaagcgg gtatttacca gccttattat aaaataaatc tgtcaaaagc
    20041 ccgaatatca agtattgatt ttgtcacacc gcatgctgtc ctggaaaaag aactggaacc
    20101 acaggagaga attgccttta tttatgaaga tatttcctgg gagcatacgt tagccggtac
    20161 gaatgcaatg agtaaatggc aggatcgtgt acagtaactt actgtaataa cataataaat
    20221 tactattgcg gagagataaa atggctggtt taagaaataa caacaacaca cagaatgcac
    20281 agtgggctga ttatgtgggt gacattcttc ggggggctca gccgataaac cagctagtac
    20341 ctcagcaccc ttatcttaac gacgtcccac tcattgatga gttaaggcat cagaacactc
    20401 atcatgttat tctcttgaca cttgatgttg caaaaaaaat actcagccca ataacatcat
    20461 ttgattacat tcattttatc actacacatc cttcaggtat aaaagataca ctcgcctggt
    20521 tagttaacgc gagtaaatta atgactgagt ttgatgacaa tgggaaaatt atatttaacc
    20581 ttaatgcctt aaagtataca aaagcaagtt actttgaaat acttggagag aaatatataa
    20641 aaatcacaac atccagcccc tggcttttag aaaagctcgg taaatatatt ttctcttcac
    20701 gagcgcctca agttctggaa ctcgctattg gctggcgagg agctttatct gaatcaataa
    20761 aaggagttaa gttctgtatc tggttttcag tagcctggag aaccatagaa tttattatga
    20821 gttcagagcg agatctggtt aattttcttg gcgatttttc aatggatgtg gccaaagcag
    20881 ttatagccgg tggtgttgca accgctattg gaagcctgac ttcttttgcc tgtgttagct
    20941 ttggctttcc agtaattctt gtcggaggag caattttact gacagggata gtgtgtacag
    21001 ttgttttaaa tgaaatcgat gctcaatgcc atttatcaga aaaattaaaa tatgcaatta
    21061 gagatggact aaaacggcaa caggaacttg ataaatggaa aagggaaaac atgactccat
    21121 ttatgtatgt tcttaacact ccacccgtga tatgaggatt aaccatgcgt ttacatgtta
    21181 aattaaaaga gtttctttcg atgtttttta tggccatatt atttttccct
//
LOCUS       DAZGVQ010000087        20937 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000087 DAZGVQ010000000
VERSION     DAZGVQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.088"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            58..1542
                     /locus_tag="ACHLAG_003090"
     CDS             58..1542
                     /locus_tag="ACHLAG_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFP0656274.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(1864..2466)
                     /locus_tag="ACHLAG_003091"
     CDS             complement(1864..2466)
                     /locus_tag="ACHLAG_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656275.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSTALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(2553..2759)
                     /locus_tag="ACHLAG_003092"
     CDS             complement(2553..2759)
                     /locus_tag="ACHLAG_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0656276.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            3495..3638
                     /locus_tag="ACHLAG_003093"
                     /pseudo
     CDS             3495..3638
                     /locus_tag="ACHLAG_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
     gene            complement(3979..4176)
                     /locus_tag="ACHLAG_003094"
     CDS             complement(3979..4176)
                     /locus_tag="ACHLAG_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656277.1"
                     /translation="MVIRKKKCRDCGKTITHNTVCCPYCGSVDPFGYYRNTDRLLCLL
                     ALLLVLILVTVSGVSVFVLLP"
     gene            4376..4945
                     /locus_tag="ACHLAG_003095"
     CDS             4376..4945
                     /locus_tag="ACHLAG_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFP0656278.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKRREGKRVHPTTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRDPGSLAGMIKQAWCSALGVDTGDHTTLAHFPVRPAVWLERDDDTGFQ
                     QVLARADYLAKESTKAYGTGERNFGCSRS"
     gene            5204..5587
                     /locus_tag="ACHLAG_003096"
     CDS             5204..5587
                     /locus_tag="ACHLAG_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP0656279.1"
                     /translation="MYAKSFIAFDGNGRLTGARTAQAAPYAHYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHGHECPYVRPERREIRLIKRLQQFVPDALPVVRKASWYCRQC
                     HHDYYGEQYCTHCQTGRFSEEVVAG"
     gene            5584..6009
                     /locus_tag="ACHLAG_003097"
     CDS             5584..6009
                     /locus_tag="ACHLAG_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP0656280.1"
                     /translation="MNGIKMGLGITPGEHIISANSALSRNIRHCFCLSCRGRLILQTD
                     AQGAWFEHDLHALSAQQKAACVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYRYQPDLYQDCGGAST"
     gene            6258..6455
                     /locus_tag="ACHLAG_003098"
     CDS             6258..6455
                     /locus_tag="ACHLAG_003098"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFP0656281.1"
                     /translation="MHQDDFCIVNPGGCIIHFPANGRYILDIHDPDFSEQYQRLLERL
                     DYLKKPGTKASGQRNSGYSGF"
     gene            6483..6767
                     /locus_tag="ACHLAG_003099"
     CDS             6483..6767
                     /locus_tag="ACHLAG_003099"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656282.1"
                     /translation="MYTESNVKLSCNALWQKCVLATLYRAAATHPEIKLLQFKLIPGQ
                     GINKAIPSLMADFTGELHRLISEYLQCKRRKDKSVEPPPFILSLEQGGCG"
     gene            6760..7050
                     /locus_tag="ACHLAG_003100"
     CDS             6760..7050
                     /locus_tag="ACHLAG_003100"
                     /inference="COORDINATES: protein motif:HMM:NF023154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3296 domain-containing protein"
                     /protein_id="HFP0656283.1"
                     /translation="MANDTTPYLMVLLLNNDVWCKSREVVVLRNLIEQAWGKVLGEDN
                     SITHLVVYPEVLSLVFDAANTYNAEPGVLEQTARAYIESHGAVERHLGCSDF"
     gene            7400..7897
                     /locus_tag="ACHLAG_003101"
     CDS             7400..7897
                     /locus_tag="ACHLAG_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656284.1"
                     /translation="MQITEALLSEPGEIRRFVQQAVDHWPHLLAIHFTLHSTEGVCGQ
                     QIQTFCTSFYELITVRNHTASPSSPVVLRWLREQHGGATIRCLLLLSQTSVCHPRASV
                     TVDEACSQVADLLQQTWPVISAGGQCRVERCFRVVRPDTSEQYVALKTAVQSLMPRGI
                     ATIIR"
     gene            7986..8609
                     /locus_tag="ACHLAG_003102"
     CDS             7986..8609
                     /locus_tag="ACHLAG_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0656285.1"
                     /translation="MHTRIDYLADKYCFTELNESSRLRLQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRAPQQIAALREEWDISQKNVMFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVIGNENTSSPYQQITREVKAPRERLKHALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKIPAYHL"
     gene            8705..8938
                     /locus_tag="ACHLAG_003103"
     CDS             8705..8938
                     /locus_tag="ACHLAG_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656286.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLADNRNRLVEVVTRKL
                     QDSFYINVSWDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(8991..9182)
                     /locus_tag="ACHLAG_003104"
     CDS             complement(8991..9182)
                     /locus_tag="ACHLAG_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0656287.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTVSSVEETRVVFMRDGYPHPCM
                     RPMYNFLGKFKPDPREETE"
     gene            9631..9759
                     /locus_tag="ACHLAG_003105"
     CDS             9631..9759
                     /locus_tag="ACHLAG_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656288.1"
                     /translation="MADLLLSILVPVDAGKYRKGGSVPSQNMVGGIVRRLVIVGMT"
     gene            9863..10438
                     /locus_tag="ACHLAG_003106"
                     /pseudo
     CDS             9863..10438
                     /locus_tag="ACHLAG_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
     gene            10436..11515
                     /locus_tag="ACHLAG_003107"
     CDS             10436..11515
                     /locus_tag="ACHLAG_003107"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFP0656289.1"
                     /translation="MEIVPGEQPGESDILITRKQDKYWRLGFSLDDSGTRSTGRYLGG
                     ITFSLDNPFSLSDLLYVSATHNFPHYGGKGSKNYTAHYSVPFGYWQFSVTASDYDYHQ
                     TVAGAVEDYQYSGESQNLGMQLSRVLHRNGTQKTVLTYDVQARRSRNYINDTEIQVQR
                     RQTAAWRLGLQHRHYISQATLDAGVSWQRGTRWFGAQPAPEEIFGEATALSKILQMNA
                     SFNLPFTLAEQPFSYSFRYQRQLSNTPLTPQEQFAIGNRWTVRGFDGERTLNASNGWY
                     IRNDLAWRTPLPEQELYLGMDYGEVGGAGSEYLVGRHLVGGVVGLRGQLFSTDYDVFI
                     GRPFSKPAGFMTSDVTAGFNLFRDF"
     gene            11525..12052
                     /locus_tag="ACHLAG_003108"
     CDS             11525..12052
                     /locus_tag="ACHLAG_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFP0656290.1"
                     /translation="MRNGKYDVLSPLYSGEPVNEAEVLGAAVWLWMHSPLHRDAPLHT
                     LPDLLLPVIKHRQYVVATEQGRPVFFMSWAWLSPEAEARYLTQPAILMPQSDWNSGDR
                     MWVCDWVAPFGHTPDMSRLVRQDIFPRLCWRSLYHRGQQTGLRVMNFRGAQVSREEAR
                     QWRVQHPLAVDVPER"
     gene            12119..13249
                     /locus_tag="ACHLAG_003109"
                     /pseudo
     CDS             12119..13249
                     /locus_tag="ACHLAG_003109"
                     /inference="COORDINATES: protein motif:HMM:NF017658.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
     gene            13358..13741
                     /locus_tag="ACHLAG_003110"
                     /pseudo
     CDS             13358..13741
                     /locus_tag="ACHLAG_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13907..>20937
                     /locus_tag="ACHLAG_003111"
     CDS             13907..>20937
                     /locus_tag="ACHLAG_003111"
                     /inference="COORDINATES: protein motif:HMM:NF024728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFP0656291.1"
                     /translation="MESAGRLDISAGSLNNHQGTVVSDGLSVTLDGALDNTSGRLLSQ
                     KTLSVSGSELVSDDGLIQSGSDMTLDVQDGVLSNRNTKTRGGISSAGTLTVRAGMLNN
                     QQGFMVGQKDMTLNAGTLDNRQGVLGSQASLQISSGTLMNQKGALKAGTDMLLSGGDV
                     SNQEGTLAAGGDLNTHLNVLENQQGTVVSNGNSRLDVTRFDNQGGRLVAQQSLTLSST
                     DIINDASGLIQSGASLNLRADTLSNRNSGDRGGVISQGAMTLNAGTLDSTAGVLLSGD
                     ALSLTAGVVNNTSGQVVANGLLGWNSQALNNQSGLIQGWGISINTAGQTLDNRRGTLN
                     SLQELTVSSGAMDNRGGTVGAKTTADLSTTSLDNREGGRLVSEGELRLHTGGLQNSHG
                     QIQSVGDMLLNSVRGVVDNVSGLIRSGSAITLNALQFINRHTQNTGQGLEAQTIHITT
                     QALDNQEGSILADRVLTVMADRTLSNNDGVLSSGATLSVSGRQLTFSNRDGVVKAGQS
                     VSVDAGQLGGDGKLLSLGDMTLKSNTTFSNSGQTIANGNLTLSVNGDVSNTGSLLAGS
                     RLDLNSIRLENTEKGEISAGQTWLNVTDTLLNRGLIDGKYTRLQANTLTNSGTGRIYG
                     DAVGVSAATFNNLEENGVAATLAGCERVDLGVQTLNNRTHSLIYSAGDMHTGGMLDAN
                     GAATGKAGVLNNHSATIEAAGYLVLSAGQINNVNDHFTTERVVVSTEKVTEYQLSGSD
                     KRWSAGEPGVYVDNDSSDSLKKLHTPEGARDKFTQYDYTRTVEETRVKESDPGKILSG
                     AGMTIVADKLFNDKSQVVAGGLLDMQAGDVENVSVSGERHVTDSGTSTYYYRIRKKGK
                     DKQGEKTSQYTPPTVIQTITLKPGELTSHGQVQGSQVTLSPLKPQGTDVQTGLTGNVD
                     MTVAGTDRIPLRPVVSAGEPVILLPGQQFEVSAPQGSIHVAGPDTRLPDSSLFKTNPA
                     VNVPYLVETDPRFTNQKTWLGSDYMQKAFSQNGDNMLKRLGDGFYEQRLIREQVVALT
                     GQRYLDGYSNDEEQFKALMDAGIAFGKQYNLTPGVALTAEQMALLTGDIVWLVNTTVT
                     LPDGSTQTVQVPQVYARVKPGDVNSAGALIAGRDMVMKLDGDLFNSGKLAGKQTVQLS
                     AENIHNQAGTIQGANVSLTARTDINSTGGLLQATDSLLAMAGRDINLTTTTRTAQSDA
                     GQNHFERTSIDSVAGVYVQNDQGRLVLQAGRDMNLTAATVVNQGKDSLTQLSAGRDMT
                     LSTVTTSAQDNITWDKNNRLSQGVTQSTGSTLAGNGDVTLTAGRDMTSQAASLSAQKG
                     LALMAGHDVTLTGAQNTSSLDEYHKVTGSSGMLSKTTTTTHDVTDRRTMTGSELNGDT
                     VSIGAGHNLNVTGSSVAGDNRVSLAAGNNLSIGTLTESNRETHLKQEKKSGLMSSGGV
                     GFSVGSQSLKVTDTATDTTQKGSTVGSVHGDVSLQAGNRLTVNGSDLIAGRDMALSGK
                     EVSITAATDQHVQAHTVEQKTSGLTLALSGTAGSALNTTVDTVQAAKSAGNSRLEALQ
                     GVKAALSGAQAVQAGRLADVQGADAGNNNTVGISLSYGSQSSKSEQQSEQTVAKGSTL
                     TAGNNLSIQATGSGVKGVDGDLTIQGSQIKAGNNILLQANRDVNLVSAENTSKLEGKN
                     TSSGGSVGVGVGVGSGGWGISVSASANQGKGSEKGNGTTHTETTVDAGKQLTIISGRD
                     TTLTGAQAGGETVKVDAGRHLTLTSEQDSDRYDSKQQNASAGGSFTFGSMSGSASVSL
                     SRDKMHSNYDSVQEQTGIFAGRGGFDVTTGQHTQLNGAVIASTATADKNRLDTGTLGF
                     SDIENRADFKTEHQSAGLSTGGSVAGNFLGNMANNLLVGANHEGHADSTTQSAVSAGN
                     ITIRDTQSQKQDVADLNRDAAHANQTLSPIFDREKEQQRLQQAQLIGEIGNQVADIAR
                     TEGQIAGEKAKRDPAALNQARAELEAAGKPFTEQDVAQRAYNNGMAASGFGTGGKYQQ
                     AIQAATAAVQGLAGGNLSAALAGGAAPYIAEIIKQTTPDGAGRVAAHAVVNAALAVAQ
                     GKNALAGAAGAATGEVVGMIATQMYGKPVSELSETEKQTVSTLATVAAGLAGGLVGDS
                     GVSAVAGAQSGKTTVENNALSFGDGFESNAAAATSWNKYAVDNNLTPEQTQAGLDKIA
                     KGDMPDSTNITKVIVDGYQDGVMIAGAWYLGPAASAGKVLGGGLLGLAANSGYQVYDL
                     NQPQNANKSWDYLGSATSFTTGMMAPGRGVLANTGIAMGGAFFTDGPNTASLAGAGIG
                     AGLGGAFGKYAPTAVGKILGNDPVPGFIYELGGGAVS"
BASE COUNT         5125 a         4990 c         6032 g         4790 t
ORIGIN      
        1 tttgctatta atagccagaa ttgcagtgct tttatatctg tgtgtagtga ggttactatg
       61 tcattcggtc agccttgtga tgagtttccc ctgagttcgc tgcctccact cattcgtgat
      121 gccgttattg aagctcagca aattacgcag gctccccttg ggcttgtggc agcgtctgcc
      181 ctgggcgctg tttcacttgt ctgccagaat ctgattgatg tctgcagact gaatacactg
      241 cgggggccgg tctctctttt tttcctgacg cttgccgagt ccggagaacg aaaaaccgca
      301 gtggacaaat tattaatgaa gcccctgtac cagcaggaga tgcagttata ttccagatac
      361 aaaagtgaac tggctgtctg gaaaaacaag gaagagcttt taaaggctca gaaaaaagca
      421 ttgttgtcaa aactgaataa agaactccgc aaaggcgcgg atgagtcaga aaccctcaga
      481 caactggaag tacttcagaa aaacagtgcg gaagaacctg tgaggtataa attcattttt
      541 aatgatgcca cgactgcggc aattaagaat cagctttgtg gtaaatggcg ttcagtgggt
      601 attatgtcag atgaagccgg tattatattt gatggatata ccctgtctga gttgcctttt
      661 attaacaaaa tgtgggatgg ctctgtattg tctgtggaca gaaagaatga acccgaacag
      721 atgattgaaa atgccagaat gacgttatcc ctcatggtgc aacccggtct ttttgaccga
      781 tatatggagc gaaaaggttc tgttgccagg gacagtggtt ttcttgcccg ttgtcttatc
      841 agtaaacctg caaccacgca ggggaaacgc tttattaacg gagctgtgat tccgggagga
      901 tcattaacgg catttcatga acgattaatg gaactggcca gaggcagtat tgaaaaatcc
      961 agtgaggatg aacggtattg tctgcatttt tcgcctgaag cgcaaaaaat atttattgag
     1021 cactataatg ttctggagca ggatttatcc ccttccggac ccttaagccc gtttcgggga
     1081 catgtttcaa aaaaaacaga aaacattgca agaattgcag cgttatttca gtatttcagc
     1141 tatggtgaag ggaaaatctc tgccgatatc atgacgtctg ctgtcgtaat aagttcgtgg
     1201 tacacagatg aatataaaaa attatttgcg ttacctgatg aatctgaact gcagcagaag
     1261 gacgccgagg agctgttcga ctggctcatt gaggagtgcc gcggagaatg cccgccccgt
     1321 gtcaggaaaa attatattct ccagtgtggc ccgggccggt tcagaaacag gaaaaaactg
     1381 aacgcattgc tgaacattct tgaatctcag ttcaggcttt ctgtcgtgcc ggaaggaaaa
     1441 accatgtatg ttctgcttcc ccagatagcc agtttaaagt tgtctgatgt ctcaggtatt
     1501 tttacctcgg gttatcacta caataagctg agagcgaagt aaccggtgag cagtttttct
     1561 ccattatgct tcagacttag atgattgatt attatgggga tttcatcacc gatattcaga
     1621 tgtatgaata tcggtgatga actaaagctc aggagctgcg tggtggtgat gaatcaacag
     1681 gcatataact gatacccgcc ggtcagaagt tccagcctgc gactttcctg tctacgtgcc
     1741 cgcagtccgg cgttcaggtg cagcgtgtga cagaacgcac ggagagcgaa acggcaggca
     1801 gacaggccgt tcagtcagcc ctggcaccct gcccggatac cagcttccct gaaacaactg
     1861 gcatcaggtg cgcttatcgt caaccgttgt gcggtatgcc aggagcacgt actgatccat
     1921 cagatccgtc agtgctgtgc tctgcttctg acggttgatg atccattcct ccagctcttt
     1981 ttctgatttc cgtttcagat tctccgtcca gtcgtcaagg cagtttccgg tcattaaatc
     2041 aagccagcaa agccagacca gtttgaggcg caactccgtg ccaccaaact gacggaagag
     2101 gctgatcagt ttatttgcca gcgaacgctg cctcagacgt tcacattcag acaggagaat
     2161 gccttccagc atcggatgat gaaccagcag atgttcactg gtgcataaat tcactatcag
     2221 atcatcatcc aggggcggcg tggtccgttt gtccggacca taaatcagcc ggtgcagact
     2281 ctggggtacg atggcaatct ccggtttttc accattgaat gacacccacc cgggcgtcag
     2341 attatggaag tagtagatat tcggatgaca cacgccccag gatacggcgt gccgggtgaa
     2401 gccggtcagc catttctggt agctttcgtg gagcagctcg taacgggtct gatactgtgg
     2461 tttcattttc atattcctca cgggggataa caggcagcag aacgccgccg gcaggccgga
     2521 gactgcgtct gttgcagatg aacggttaag aattacggac gggactgtgc aatacgcgcc
     2581 tgcagccagg cttccacttc gcttttcagc catcgggagc tgcggcccat tttgatgggc
     2641 gcaggaaagt caccatcctt gatgagtttg taaaaccact tatcggtcag gccggtcagt
     2701 tgagtgataa atgccatgtc gaccatctgg tcatccatca gtgaaactgg ggtgatcatg
     2761 agaatgttcc tccggatgtt gttaaaccag ccggtgtggt catgaaaaca acgcaccacc
     2821 ttcaccggca taacaaccgg ggagaaacgt aaaaaataat cgggaatgtg accgccagga
     2881 tataaacggg gaggttacgg tggctgagcc gtaccggtat gccgggtggt tgttacgaaa
     2941 cgatgcttcc gcacgcacgc gcgataaaga agggatgatt ttcgtgagga agaggggaaa
     3001 actggcgttc tgataattgc agagttattt acggggcggt tacggtgggg cagcccgtgc
     3061 cttgtgggca gggacaggta tcactggtgg gataaggctg tctgattggt attataaata
     3121 taagaagtta tcagagagta acgtaaagga tttgccagaa taacctaaga gagtaaatct
     3181 ctccggtaac agagcgctgt gaagcattct gtatcagagt gcaaatacat gaacgctgat
     3241 gcgtaaaatc agcagtgaca cgccagctcc ccctatgccc attcctgtat aagacatgaa
     3301 ctgagcgtat taatgctcct gccctgaaac tgagccggtg agtgaggggc tgagaaatcc
     3361 ctcttgacac tgctctaacc agaaatactt ccccggttgt tgcagtgcgt ctggatcccc
     3421 gataacggaa cactaaggct gtattgcggg acaccaggta agtggcagcc cgtaatacgt
     3481 caggagacgc gggaaaagtg aattcccaca cgcgttataa acttacccta gcggagctgg
     3541 aagcctttaa ctctgccgtt gacaaccggc tggcagaact aaccatgaac aaactttacg
     3601 atcgcgttcc ggcttctgtc cggaaatatg tcacctgaac gttcggtaat cacagcggta
     3661 ttgcgccagg tgttatctgc tctgtgtgat tctttttagg gtttctcaga ttgttaaaag
     3721 ctgaatatgt tcctgttgtc gggaaccagg ataatacagg gaacattaag cggtatagcg
     3781 acaacaaagc attcgaaatc aaaagggaat ggtgtacgga gttgtgggca gtaatgggcc
     3841 gattttcggg cattaccgat ctctgttgag gttttctcgt atttgccggg aatccggtgc
     3901 gttactgtct ggcacagcaa acgcagaggt ggcggggcgc tctgctccgc tgattccgga
     3961 taatccctcc cctgtaacct acggcaacag tacaaataca ctgacgcctg aaacggtaac
     4021 cagaatcagc accagcagta acgccaataa acagagcaaa cgatcggtat ttcggtaata
     4081 accgaaggga tcgacagagc cacaatacgg acaacacacc gtattatgag tgatggtctt
     4141 accacaatcc ctgcacttct ttttgcgtat caccataact ccctcctcac agccttaccc
     4201 gtaaccggtt tttgacatta aaaaaacaac gctgaaaatt caaccgtcag tccggagacg
     4261 ctcgttcggg ttatcacaga gtgcctgaga cagtgtcctg ccggaggtca cttcagggat
     4321 tctgcgcatt ttttacgacg tggctattcc gtatgaacca tacggagatt taaccatgac
     4381 ctacaaatac aaccccttct ggcagcaacg tattcgtgag acagtgcggc atgcactgaa
     4441 tgttcatccc cgcctgacgg cattgcgggt tgacctgcgt ctcccggatg taccggcagc
     4501 aacggacgca gctgtgatat cccgcttcat caatgccctg aaagcccgaa tcgacgctta
     4561 ccagaagcgt aagcgccgtg aaggtaaacg cgtgcatccc acaacactgc attacgcctg
     4621 ggcccgggag ttcggggagc taaaaggcaa aaaacactat cacctgctgc tgctggtcaa
     4681 ccgggatacc tggtgtcgtg caggtgatta ccgcgatccg gggtcactgg ccgggatgat
     4741 taaacaggcc tggtgcagtg ccctgggtgt ggataccgga gatcatacca cgctggcgca
     4801 ttttccggtc aggccggcgg tgtggctgga acgcgatgat gacaccggct ttcagcaggt
     4861 gctggcgcgt gctgactatc tggcgaagga gagtaccaaa gcttacggca ccggtgagcg
     4921 taattttggc tgtagccgga gttgagcacg actggcgcgc caatacccgg caacccaaaa
     4981 gtatgtgttg ttgtctggcg gcaacgatgt atccactcgc ccactgactg ttttttacgt
     5041 cagagttcca ttgtgtgaca accacagtgc tgtaacagca accagcctgt tttacccaaa
     5101 ctccagtcca gattttaact gaaccgccat tcacaccctg agcacactgc ccgtaccggg
     5161 acggctgttg cctgcgtgtc gtctggcgct gaggaatatg acgatgtacg caaaatcctt
     5221 tattgcattt gatggcaacg gacgtctgac gggcgcccgt actgcacagg ccgcacctta
     5281 tgctcactac acctgccacc tttgcggcag tgcactcaga taccacccgc aatacgacac
     5341 tgaacgtccc tggtttgaac acactgacga cgggctgact gagcacggtc atgagtgccc
     5401 ttatgtcaga ccggaacgca gagaaatacg tttgattaaa cgtctgcagc aattcgtacc
     5461 ggatgcctta cccgtagtac gtaaagccag ctggtactgc agacaatgtc accacgatta
     5521 ttatggggag cagtactgca cacactgcca gaccgggcgc ttcagtgagg aggtggtagc
     5581 aggatgaacg gcataaaaat ggggctgggg atcacgccgg gtgagcacat tatcagtgcc
     5641 aactctgcgc tcagcagaaa tatccggcat tgtttctgcc tttcctgtcg tggccggttg
     5701 attttgcaga cagatgctca gggggcgtgg tttgagcatg atttacatgc cctgagcgcg
     5761 caacagaagg cggcctgcgt ggtgctgaat ccggaaaagt cccaccctta cattgaggat
     5821 atggcgatgt ttctatcgcc attaccggtc gtgctggagt ggcattgtgt gatgtgtgag
     5881 cagttttttc atgggaagaa attctgtgag gcctgtggta cggggattta ttgcagagcg
     5941 gtatgtacga agtctgttta caggtaccag ccagatttgt accaggactg cggaggtgcg
     6001 agtacttaat ccaaaatatc cgctctgcgc agggcgttca gaggtattgc cagcctgcat
     6061 tttatccggt gtttcgcacc tgccccgttc gtgacacaaa ggcgcgacac cggatatccg
     6121 cagtcctgct tacctgaata aaacccgggg tcatgattgt gtgctcgtgg aacgaaatcg
     6181 caccgtgatc gcggattcgc cgaatctgca ctgtggtgtc aataaaccac acgcgagagt
     6241 cctctccgta ttgatcagtg catcaggacg atttctgcat tgttaatcca gggggctgca
     6301 ttattcattt ccctgctaat ggacgttaca tcctggatat tcatgatcct gatttctctg
     6361 agcagtatca gcgcttattg gagcgtctgg attatctgaa gaagcccggt actaaagcat
     6421 caggccagcg taactctggc tacagcggtt tttgacctgt tgtaactgta aggtgattta
     6481 ttatgtatac agaatcaaac gttaagcttt cctgtaacgc cctctggcaa aagtgtgtcc
     6541 ttgcaactct gtatcgggct gcagccacac atcctgaaat taaattatta caatttaaat
     6601 taataccagg gcagggtatt aataaggcca ttccctctct gatggctgat tttactggtg
     6661 aactgcatag actcatttct gaatatttgc agtgtaagcg acgtaaagat aaatctgttg
     6721 agcccccccc ctttatatta tctctggagc agggaggctg tggctaacga taccactcct
     6781 tatcttatgg tgctgctact taataacgat gtctggtgta agagtcgtga ggtggtggtt
     6841 ctcagaaatc tgattgagca ggcgtgggga aaagtattgg gtgaagataa ctccattaca
     6901 catctggttg tgtatcctga agtgctgtcg ttggtatttg atgcagctaa cacatacaac
     6961 gctgagcctg gtgtactgga acagacagcc cgggcttata ttgaatcgca tggtgctgtt
     7021 gaacgtcatc ttgggtgtag tgacttttaa cgtcaggtga cgcattgtca cttctgacct
     7081 gctacctgta cgccggaata taatggtcag agaggcatat ttcgacgccc ctaatctggg
     7141 gcgtttgatt caggatgtct ggaacaatcc gggctttgtg tcaccgggcg catcaaagca
     7201 gtaccacccg ggatgtggcg ctgttccctg gtgctggtcc ccccgtgaat cttcctgcgt
     7261 cccctccggt cagccggcac tttgtcatcc tgactgattc atcattcctt tcatattgcc
     7321 agtcacagct gtttgcatcg aaccgtactt gtgttgatga gtctgcaccg acttaaccaa
     7381 attcaacaga gataaaatag tgcaaataac cgaagcctta ctttcagagc cgggggaaat
     7441 ccggcgtttt gttcagcagg cggtggatca ctggccacat ctgctggcaa tccacttcac
     7501 acttcattcg acagaaggcg tctgtggtca acagattcag acattctgta cttcctttta
     7561 tgaacttatt actgtgcgta atcacactgc cagcccatca tcgccggtgg tattacgctg
     7621 gttgcgggaa caacatggag gagcaacaat acgatgcctg ttactgctca gccagacgag
     7681 tgtttgccat ccgcgggcca gtgtcacagt tgatgaagca tgttcgcaag tggcggattt
     7741 actgcaacag acctggccgg tgataagcgc tggcggacaa tgccgggtgg aaaggtgttt
     7801 tcgggttgtc cggcctgata catcagagca atacgttgcg ttaaaaacgg ctgttcagtc
     7861 tctgatgcca cggggtattg caacaatcat ccggtgatgg acgggagaaa tatcgactct
     7921 tccgttagtt tttacagact gataaagttt tgccagctga atctttatac ggaatactct
     7981 tcagtatgca tacacgaatt gactatctgg cggataaata ctgctttaca gaactgaatg
     8041 agtcttcccg tcttcgcctg cagtggcagg atgtcctgga ggagtgccgg cagacagaag
     8101 ccggaccaga agaacgactt cgcattgccc tgttgaatgt ggattatgtc accagttttg
     8161 aactgccttt tcgcttgttg cttacccgtg caccacaaca gattgccgcg ctgcgtgaag
     8221 aatgggacat cagccagaaa aatgtgatgt tcaacgataa acgctttggc tgcgtgtaca
     8281 gcctgaaggc cagcctttcc ggtgtgccgg atacataccg gtatcatctg tctcatcgta
     8341 ttcgccgggt gattgggaat gaaaacacat catcgccata ccagcagatt acccgggaag
     8401 tgaaagcgcc ccgtgaacgg ctgaagcatg cgctggaggc ggggttgctg gtgactgcac
     8461 tggacggcct gttctggttc ggcagtcagc gtattgcagc tgatgttttg aggctgagaa
     8521 aggccggaat gccggtggtg actacaaccg tggaggtaca cgataacctg acgggaacca
     8581 cccgcaaaat accggcatac catctctgat acggccatca gggggaatat tgctctggta
     8641 gctgcagtac gacattcttt atactttatc cccgtgcctg aaaacatgtg ctaaggaaac
     8701 gggaatggca aggtccgatt atgatattat caatctgtct ctggaacatg aactgaatga
     8761 gtggctggca gagagaggtt atgccggact ggcggataac cggaatcgcc tggtagaagt
     8821 tgttacccgc aaattgcagg acagctttta tataaacgtt tcctgggatg cgctgaacac
     8881 ggcatacagt gaacacccgg agtggttttc tgggcttgtc tccggggatg agaattaata
     8941 aatggattat gctgccatca ggcagtgtga ttgccggcag ggatttcgtt ttattctgtt
     9001 tcttcccggg ggtcgggttt gaattttccc agaaagttgt acatcggtcg catgcaggga
     9061 tgcggatagc cgtcccgcat aaacaccacc cgggtttcct caacactgct cacggtgaca
     9121 agcgcaccat gatcgtcttt atatcgctga ttgactttcg gtaatggaac atcttctgac
     9181 ataaatttta gtacggtaac agttaactaa attttcatgt ggtagcactg aatcaggaga
     9241 ctgagtttcc tgatgtgcga tgttaatttt ctgtttttac gaaacatttt ccccttcttc
     9301 ctgtatattt tattgcgctc ctcctttcat gtccttccca tatcctgaaa ctcacttaaa
     9361 tgctgttgaa tcgtcacgca ttgcaatttt caatgaccga aatcaaaaaa taatattttt
     9421 tttagctaac caactgtatt aaaagcattc agtggaataa ttttctgcac ttttccatta
     9481 atcatggtta taaattttgc aatgtgctgt ttttgcgata tatgacagaa taaagaactg
     9541 ttcatttgtg ttatatcgat cgataaatct gtcatattgc tgtagttcag atcattattc
     9601 tataatgatc aaaaataagt cagctaaaaa ttggcagatt tattgctcag cattctggta
     9661 ccggttgatg cgggtaaata ccggaagggc ggtagcgtgc catcgcaaaa catggtggga
     9721 ggtatcgtca ggaggctggt gattgttggt atgacatgaa taaaaggttt ttcgtttgac
     9781 tggaaaatta tgttgcagga acgagagggc tgaggaacaa agcttattcc ggcgctgtcc
     9841 gtccttgctg tcactgacac tgatgtctgc tttattatcg cctttatctc ttcaggccgc
     9901 ggatatccgg cgtagtggag aggatacatt tatcattcag cagcagcgtc aggaagccct
     9961 tgagcaacaa ctgacgcctt cagcccctga tgttcgcctt tctgcacctg gctcttttgc
    10021 ccataagatt aattttcctg ttgaaacgcc ctgttttcag attaaacaga cggaactgaa
    10081 gggggctgat gcgttaccac actggctgcc tttacaaaaa atcgccaacg gggcggtcgg
    10141 gcattgcctg ggggcgaaag gaattaatct gctgatgagt acattgcaga accgtctggt
    10201 cgatcatggt tatgtcacca cccgtgttct ggcaccttcg caggatttaa aaagcggtat
    10261 cctccggctg gttattattc ccggtgttgt gcgacatgtg cgtctgacac cggacagtga
    10321 tgactatatt cagttgtatt cctcattccc ggcacacgaa ggttctctgc tggatttacg
    10381 ggacattgag caggggctgg agaatatgca acgactgccg ggtgtgcagg ctgatatgga
    10441 aattgtgccc ggtgagcagc caggggaaag tgatattctt attacccgta aacaggataa
    10501 atactggcga ctggggtttt cgcttgatga ttcaggtacc cgcagcacag ggcgttatct
    10561 cggtggtata acattttctc tggataatcc tttttccctg agtgacctgc tgtatgtttc
    10621 tgccacccat aattttccac attacggggg taagggcagt aaaaactaca cggcccatta
    10681 ctctgtgcct ttcggttact ggcagttttc cgtcaccgcc agtgattacg attaccatca
    10741 gacggtagct ggtgcggttg aggattacca gtacagtggc gagagtcaga atctgggcat
    10801 gcagctgagc cgggtactgc accgtaacgg gacgcagaaa acggtgctga cttacgatgt
    10861 tcaggcccgt aggtcgcgta actacattaa tgatacggaa atccaggtgc agcgccggca
    10921 gacagcggcc tggcgacttg ggctgcagca ccgtcattat atttctcagg ccacactgga
    10981 tgctggtgtc agctggcaac ggggaacccg ctggtttggt gcacagccgg caccggagga
    11041 aatcttcggt gaggcaacgg cgctcagtaa gatcctgcag atgaatgcgt cctttaatct
    11101 gccctttaca ctggcagagc agcccttcag ttattccttc cggtatcagc gccagctgag
    11161 caataccccg ctgaccccac aggaacagtt tgccatcggt aaccgctgga cggttcgtgg
    11221 ttttgacggg gagcgaacgc tgaatgccag taacggctgg tatatccgca acgacctggc
    11281 ctggcgtacg ccactgccgg agcaggagtt atatctggga atggattacg gcgaggtggg
    11341 gggagccggt agtgaatacc tggtgggacg tcatctggtc ggtggtgttg tggggctgag
    11401 ggggcaactg ttcagtacgg attatgacgt tttcatcggt cgtccttttt caaaaccggc
    11461 gggttttatg acgagtgatg tgacagccgg ttttaatctg ttccgggatt tctgagaaaa
    11521 tttcatgcgt aacgggaaat atgacgttct gagtcccctg tattccggtg agccggtcaa
    11581 tgaggcggaa gttctggggg cggccgtctg gctgtggatg cactcccccc tgcatcgtga
    11641 tgcgccattg cataccctgc cggacctgct gctgccggtg attaagcacc gacagtatgt
    11701 ggtggcaaca gagcagggac gaccggtttt cttcatgagc tgggcctggc tcagcccgga
    11761 ggcagaggcg cgttatctta cgcagccggc aattctcatg cctcagtcgg actggaacag
    11821 cggcgaccgg atgtgggtct gtgactgggt tgcccctttt ggacacactc cggatatgag
    11881 tcgtcttgtc cggcaggata tttttccccg tctgtgctgg cgttcgctgt accaccgtgg
    11941 gcagcagacc gggttgcggg tgatgaattt ccggggtgcg caggtcagtc gtgaagaggc
    12001 gcgacagtgg cgtgtgcagc acccgcttgc tgtggatgtg ccggagagat aagtcaggtt
    12061 tatcagaatg aacagaaatt gttaccgcat tatttttaac aaagcccggg gaatgctgat
    12121 ggtggtggca gatattgccc attccggtcg cgccggtaca tcactgtcct cccgcacagg
    12181 atatccacac aggcaacgta tctgccgtgt cacgccgctt gcattctctt tgtggctggc
    12241 gtcggggatg gtgcattctg tcagtgctgc aggcattgtc gcagaccatg gcgcaccggg
    12301 gcatcagcag cccacgatta cgcagacggc cagcggcatt ccgcaggtga atattcagac
    12361 gccttctgcc ggcggggttt cacataatac atacagccag ttcgacgtcg gtaaccaggg
    12421 cgtcattctg aataatgccc ataacaatgt gcagactcaa ctgggtggca tggtggccgg
    12481 gaacccgtgg ctggccaagg gagaggccag aatcattctt aatgaagtga acagtcgcaa
    12541 tcccagccag ttgaacggtt ttgttgaggt tgccgggaaa aaggcgcagg tggtgattgc
    12601 gaacccggcc ggcatcagtt gcgacgggtg cggttttatt aatgcgaacc gggccacgct
    12661 gaccacgggg cagcctcaga tgaaaaacgg cagtctgacc ggattcagtg ttgaacgtgg
    12721 tgagattcag attacgggca aagggatgga tgcctcgcgc acggattata ccgatatcat
    12781 tgcccggtct gtgaagatta atgcgggtat ctgggcgcag gacctgaaag tgaccacagg
    12841 acgtaacaat gtggatatcg cgcacgggca gattgagaaa aagaccggag atgtctcttc
    12901 acagcctcag atggcactgg atgtggccag tcttggcgga atgtatgccg gaaaaattcg
    12961 ccttgtcggt acggaaacga gggtgggtgt tcgtaacgcc ggtcatattg gtgcacaggc
    13021 aggggcggtt accctgacgg ctgacggacg aattgaaaac agtggcagta tcagtgcgaa
    13081 aacggatgtg catctggcga caacccggga gttgcataac agtggttctg tttatgcggg
    13141 gcaggatacg cagatacaga gtgatggtgt attcacccat acaggctctg tggcatcacg
    13201 tcgcaacacc cggattcaga cagcgcgtct gatcggcgat gagcggagtc tgctggccgc
    13261 cggggtgaaa gatgacggac gtctggcggc agcgggcaac ctgactgtca gtaccaccgg
    13321 cgaactggcg gcacacggac aggttctgtc cggtggggac atgcaactga aggggcaggg
    13381 gctggattta ggcaacagcc ggatacaggg acaacatact gagctgaatg caaccagtgg
    13441 aaatctgtct acacagaatg cgcaactgag tgccggtacg ctttccgccc ggactgccgg
    13501 gcagttcagc aataatggcg gtacgataaa tgccgacaca ctgcagataa cggcacaaag
    13561 cctgtcaaat cgtaaaggca gtctgattca gacgggaaca ggggattttt cgctgagtct
    13621 gccgggaagc gtggataacc gggaagggct gcttgcggca aatggcgcgg tgcgtctgga
    13681 tgctctgagc cttgataatc gcaaggggaa agtgcaggcg gcgcagtcag gcagtctgca
    13741 ggtgaagaca accggtgccg tggataacca gcagggacgt ctgcttgcca gcagtgacat
    13801 cctgattaat acacaggcgc tgaataacga caacggactg atttcagctg ctgccggaac
    13861 gggcaggata aaaacacagc aggctgtcag caatacggag ggacgaatgg agtccgccgg
    13921 caggctggat atttcggcag ggagtctgaa taaccaccag ggtacagtgg tgtctgacgg
    13981 gctgtccgtg acgctggatg gtgcgctgga taatacgtcc ggcagactgc tttcacagaa
    14041 aacgctgtct gtgtcaggca gcgaactggt ctctgatgat ggtctgattc agtccggcag
    14101 tgacatgacg ctggatgtac aggacggggt acttagtaac cggaacacga aaacacgggg
    14161 tggtatcagc agcgccggta ccctgacagt gcgggctggt atgctgaata accagcaggg
    14221 atttatggtg ggtcagaagg atatgaccct gaacgccgga acgcttgata accgtcaggg
    14281 cgtgctgggg agtcaggcgt cattacagat atcgtcaggt acgctgatga atcagaaagg
    14341 tgcactgaag gccgggacgg atatgttgct gagtggtggc gatgtcagta atcaggaagg
    14401 gacgcttgcc gccggcgggg acctgaatac tcacctgaat gtgctggaaa atcagcaggg
    14461 gacggtggtc agcaacggta acagcaggct ggatgtcaca cggtttgata atcagggcgg
    14521 cagactggtg gcacagcagt cactgaccct ttcctcgacg gatatcatca acgatgccag
    14581 cggcctgata cagagcggtg catcgctgaa tctccgtgct gacacactga gtaaccgtaa
    14641 cagtggtgac cggggcgggg ttatcagtca gggggcgatg acgctgaatg ccggcacact
    14701 ggacagcact gccggtgtgc ttttgtcggg agatgctctg tccctgactg caggcgtggt
    14761 taacaataca tccggtcagg tggtggcaaa cggtctgctc ggctggaaca gccaggcgct
    14821 gaacaatcag tccggtctta ttcaggggtg gggtatcagc attaataccg caggccagac
    14881 gctggataac agacgcggta cgctgaacag cctgcaggaa ctgactgtca gttccggagc
    14941 aatggataac cggggcggga ccgtgggggc gaaaacgaca gctgacctga gcaccacctc
    15001 gctggataac cgtgagggtg gccggctggt cagtgaaggt gaactccgtc tgcatacggg
    15061 aggtctgcag aacagccacg gacagataca gtctgtgggc gacatgttgc tcaacagtgt
    15121 acggggcgtt gtggacaacg tctctggtct gattcgcagt ggttccgcca ttacgctgaa
    15181 cgccctgcag tttatcaatc gccacacgca gaataccggc caggggctgg aagcgcagac
    15241 aatacacatc acgacgcagg cccttgataa ccaggagggc agtattctgg cagacagggt
    15301 gcttacggtt atggcagaca ggacgctcag taataatgat ggcgtgctct cttccggcgc
    15361 gacattgtct gtcagtggcc gtcagctgac gttttcgaac cgtgacggcg tggttaaggc
    15421 cgggcagtca gtatctgtgg atgccggtca gctcggtggt gacgggaaac tgctttcgct
    15481 gggcgatatg acgctgaaaa gtaacacaac gttcagcaac agtggtcaga ctattgcaaa
    15541 cggaaacctt acgctttccg ttaatggcga tgtctctaat accggcagcc tgctggcagg
    15601 cagccggctt gacctgaaca gcatccgcct tgaaaacaca gagaaaggtg aaatcagtgc
    15661 cgggcagacc tggcttaacg tgacggacac actgctgaac cgtgggctga ttgacgggaa
    15721 atatacgcgt cttcaggcga acaccctgac gaattccggt accgggcgaa tctacggtga
    15781 tgctgttggt gtcagtgctg ccacatttaa taatctggaa gaaaatggcg tggcggcgac
    15841 actggccggg tgtgagcgtg tggacttagg ggtacagacc ctgaataacc ggacgcacag
    15901 cctgatttac agtgcaggtg atatgcatac tggtggcatg ctggatgcga atggcgcagc
    15961 cacggggaaa gccggtgtat tgaataatca cagtgccact attgaagccg ccggatatct
    16021 ggtactgagt gccggacaaa tcaataacgt caatgaccat ttcaccacgg agcgcgtggt
    16081 ggtttccacg gaaaaggtaa cagagtacca gctcagcggc tcagataaac gctggagtgc
    16141 tggtgaaccg ggtgtttatg tggataacga ttccagtgac tcactgaaaa aactgcatac
    16201 accggaaggc gctagagata aattcacgca gtacgattac acccgtaccg tggaagaaac
    16261 ccgggtaaaa gaatccgacc cgggaaaaat tctgtccggt gccggcatga cgattgtggc
    16321 agacaaactg tttaatgata agagtcaggt tgtggcaggt ggcctgctgg atatgcaggc
    16381 cggtgatgtg gaaaacgtca gtgtcagcgg agagcgccat gttacggact cagggacatc
    16441 cacgtattat taccgtatca ggaaaaaagg aaaggataag cagggggaga aaacctctca
    16501 gtacacaccg cccactgtaa ttcagaccat cacgctgaag ccgggagagc tcaccagcca
    16561 cggacaggtt cagggtagcc aggtcacgct ttctcccctg aagccacagg gaacggatgt
    16621 acagacagga ctgaccggaa atgtggatat gacagtagcc ggtactgacc ggataccgct
    16681 tcgcccggtg gtgtctgccg gtgaaccggt cattctgttg ccggggcagc agtttgaagt
    16741 cagcgcacct cagggcagta tccatgttgc cgggccggat acccgtctgc cggacagcag
    16801 cctgtttaaa acaaatccgg cagtgaatgt gccatatctg gttgaaacgg acccgcgttt
    16861 tacgaaccag aagacgtggc tgggcagtga ttacatgcag aaggcgtttt ctcagaacgg
    16921 ggataacatg ctcaaacgcc tgggggatgg tttttacgaa cagcgtctga ttcgtgagca
    16981 ggttgtcgct ctgaccgggc aacggtacct ggacggatac agtaatgatg aagagcagtt
    17041 taaggcgtta atggatgcgg gtattgcatt tggtaagcaa tacaatctga caccaggtgt
    17101 ggcgctgact gctgagcaga tggcgttact gaccggtgat attgtctggc tggttaacac
    17161 cacggtgaca ctgccggacg gcagcacgca gacggttcag gtacctcagg tgtatgcccg
    17221 tgtaaaaccc ggtgatgtga acagtgccgg tgcgctgatt gccggcaggg acatggtgat
    17281 gaagctggat ggtgacctgt ttaacagcgg taaactggcc gggaagcaga ccgtgcagct
    17341 cagtgcagaa aacattcaca accaggccgg caccattcag ggagcaaatg tcagcctgac
    17401 ggcccggacg gatattaaca gtaccggcgg gctgctgcag gccacggaca gcctgctggc
    17461 gatggccggg cgggatatta acctgaccac caccactcgc acggcacaaa gtgacgccgg
    17521 acagaaccat tttgaacgca ccagcatcga cagtgtggcg ggcgtgtatg tacagaatga
    17581 ccagggaaga ctggtgctgc aggccggacg ggatatgaat ctgacggctg caacggtggt
    17641 gaatcagggt aaggacagcc tgacgcaact cagtgcgggc agggatatga cgctttccac
    17701 tgtgaccaca tccgcgcagg acaatattac ctgggacaaa aataatcgtc tgtcgcaggg
    17761 cgtcacacag agtacgggca gcacgcttgc cggtaacggt gatgtcacgc tgaccgccgg
    17821 acgggatatg acgtcacagg cggcatcgct gtcagctcag aaaggcctgg ccctgatggc
    17881 gggtcatgac gtgacgctga ccggtgcaca gaataccagc tcgctggatg aataccataa
    17941 agtgaccggc agcagcggga tgttatcgaa aaccaccaca accacgcatg atgtcactga
    18001 ccgccggacc atgacgggca gtgaactgaa cggggatacg gtcagtatcg gcgccggaca
    18061 taatctgaac gtcacgggca gcagtgtggc cggggataac cgtgtctccc tggctgccgg
    18121 aaataacctg agtatcggaa cgctgacaga aagcaaccgt gaaacacatc tgaaacagga
    18181 gaaaaaatcc gggctgatga gctccggagg tgtgggattc agcgtgggca gccagagtct
    18241 gaaagtcacg gacaccgcaa cagacaccac gcagaaaggc agcacggtgg ggagtgttca
    18301 cggtgatgtt tcccttcagg caggtaaccg gttgaccgtc aacggctctg acctgattgc
    18361 tggcagggac atggcactca gcggtaagga ggtcagcatt accgccgcca cagaccagca
    18421 tgtacaggca catacggtgg agcagaaaac ctccggtctg acgcttgccc tgtccggcac
    18481 agcgggcagc gccctgaata cgacggtgga taccgtacag gcggcgaaat ctgccggaaa
    18541 cagccggctt gaagccctgc agggggtgaa ggccgcgctc tcgggggctc aggccgtgca
    18601 ggccggacga ctggcagacg tgcagggcgc cgatgccgga aataataaca cggtgggcat
    18661 cagcctctct tacggcagcc agtcttcgaa atcagaacag cagtcagagc agacggtggc
    18721 gaagggcagt acactgaccg caggtaataa cctcagtatt caggccaccg gctccggcgt
    18781 gaagggcgtg gatggtgacc tgacgataca gggcagtcag ataaaggccg ggaacaacat
    18841 tctgctgcag gcaaaccgtg acgtgaatct ggtatcagca gaaaacactt caaagctgga
    18901 aggaaagaac acatccagtg gtggcagcgt gggtgtcggt gttggtgtgg gctccggagg
    18961 ctggggtatc agtgtttcag ccagcgcaaa ccagggtaag ggcagtgaaa aaggcaacgg
    19021 taccacccat acagaaacca cggtggatgc gggaaaacag ctgaccatta tcagcgggcg
    19081 ggacaccacg cttaccggcg cgcaggctgg cggtgaaacg gtgaaagtgg atgccggtcg
    19141 ccatctgaca ctgaccagtg agcaggacag tgaccgttat gacagcaagc agcagaatgc
    19201 cagtgccgga ggcagtttca ctttcgggtc catgagcggt tcggcgagtg tgagcctcag
    19261 ccgggataaa atgcacagta actacgacag tgtgcaggag cagacaggga tttttgccgg
    19321 caggggcggg tttgatgtca ccaccgggca gcatacgcag ctgaacggcg cagtgattgc
    19381 gtccacggca acggcagata aaaacaggct ggataccggc acactgggct tcagtgacat
    19441 tgaaaaccgg gccgacttta aaacggaaca tcagagtgca gggctcagta ccggtggcag
    19501 tgttgccgga aatttcctgg ggaacatggc aaacaatctg ctggtcggtg caaatcatga
    19561 aggccatgcg gacagtacca cgcagtcggc agtgtctgca gggaatatca ccatccggga
    19621 tacgcagagc cagaaacagg atgtggcaga cctgaaccgt gacgcagcac atgcaaatca
    19681 gacgctgtcc cccatctttg acagggaaaa agagcagcag cgactgcagc aggcgcaact
    19741 cattggtgag attggtaacc aggtggcaga tattgccaga acggaaggac agatagccgg
    19801 tgagaaagcg aaacgggacc cggcagcact gaatcaggcc cgtgcagagc ttgaggcagc
    19861 aggaaaaccg ttcacggaac aggatgtggc acagcgggcc tacaataacg gtatggcagc
    19921 ctccggtttt ggaacgggag gcaaatacca gcaggcgata caggccgcaa cggcagcagt
    19981 acaggggctg gccggcggta atctgagtgc agccctggca ggtggtgctg cgccgtatat
    20041 tgctgaaatt atcaaacaga ccaccccgga tggtgcggga cgtgtggccg cccatgccgt
    20101 ggtgaatgcc gcactggcgg tggcacaggg taagaatgct cttgccggtg ccgcaggcgc
    20161 agccaccggc gaggtggtgg gaatgatagc cacacagatg tacggtaagc cggtgagtga
    20221 actgagcgag acagagaagc agacggtctc gacactggca acggttgctg cgggcctggc
    20281 cggtggtctg gtgggagact cgggagtttc agcggtagcc ggcgcacagt caggcaagac
    20341 aacggttgag aataatgcgc tcagcttcgg tgatggtttt gaaagcaatg cagctgcggc
    20401 tacatcatgg aacaagtacg cggtggacaa taatctgaca ccggaacaaa cacaggctgg
    20461 gctggataag atagcgaaag gtgatatgcc ggacagtacc aatatcacga aagtgatcgt
    20521 ggatggatat caggatggtg taatgattgc cggggcatgg tatctggggc ctgctgcgtc
    20581 agcaggtaaa gttcttggtg gcgggcttct tggactggcg gccaatagtg gttatcaggt
    20641 ctatgatttg aaccagccgc agaatgcgaa taagtcgtgg gactatctgg gaagcgcgac
    20701 ctcatttaca acaggcatga tggctcccgg tcgtggtgtc cttgcgaaca caggtatcgc
    20761 aatgggtggg gcattcttta ctgatggtcc aaatacagct tcactggccg gggctggtat
    20821 tggtgctggt ctgggtgggg catttgggaa gtatgccccc acagccgtgg ggaaaatctt
    20881 agggaatgat cctgttccgg gctttattta tgagcttggc ggtggtgctg tctctga
//
LOCUS       DAZGVQ010000088        20835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000088 DAZGVQ010000000
VERSION     DAZGVQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.089"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(83..1732)
                     /gene="actP"
                     /locus_tag="ACHLAG_003112"
     CDS             complement(83..1732)
                     /gene="actP"
                     /locus_tag="ACHLAG_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFP0656292.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1729..2043)
                     /gene="yjcH"
                     /locus_tag="ACHLAG_003113"
     CDS             complement(1729..2043)
                     /gene="yjcH"
                     /locus_tag="ACHLAG_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFP0656293.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAIYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2243..4201)
                     /gene="acs"
                     /locus_tag="ACHLAG_003114"
     CDS             complement(2243..4201)
                     /gene="acs"
                     /locus_tag="ACHLAG_003114"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFP0656294.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4594..6030
                     /gene="nrfA"
                     /locus_tag="ACHLAG_003115"
     CDS             4594..6030
                     /gene="nrfA"
                     /locus_tag="ACHLAG_003115"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFP0656295.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEELARKNGLLSQ"
     gene            6075..6641
                     /gene="nrfB"
                     /locus_tag="ACHLAG_003116"
     CDS             6075..6641
                     /gene="nrfB"
                     /locus_tag="ACHLAG_003116"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFP0656296.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6638..7309
                     /gene="nrfC"
                     /locus_tag="ACHLAG_003117"
     CDS             6638..7309
                     /gene="nrfC"
                     /locus_tag="ACHLAG_003117"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFP0656297.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7306..8262
                     /gene="nrfD"
                     /locus_tag="ACHLAG_003118"
     CDS             7306..8262
                     /gene="nrfD"
                     /locus_tag="ACHLAG_003118"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFP0656298.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8282..10000
                     /gene="nrfE"
                     /locus_tag="ACHLAG_003119"
     CDS             8282..10000
                     /gene="nrfE"
                     /locus_tag="ACHLAG_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFP0656299.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFHFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            9993..10376
                     /gene="nrfF"
                     /locus_tag="ACHLAG_003120"
     CDS             9993..10376
                     /gene="nrfF"
                     /locus_tag="ACHLAG_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFP0656300.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10373..10969
                     /gene="nrfG"
                     /locus_tag="ACHLAG_003121"
     CDS             10373..10969
                     /gene="nrfG"
                     /locus_tag="ACHLAG_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFP0656301.1"
                     /translation="MKQPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASKQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11311..12624
                     /gene="gltP"
                     /locus_tag="ACHLAG_003122"
     CDS             11311..12624
                     /gene="gltP"
                     /locus_tag="ACHLAG_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFP0656302.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12702..13391)
                     /gene="yjcO"
                     /locus_tag="ACHLAG_003123"
     CDS             complement(12702..13391)
                     /gene="yjcO"
                     /locus_tag="ACHLAG_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFP0656303.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13485..15632)
                     /gene="fdhF"
                     /locus_tag="ACHLAG_003124"
     CDS             complement(13485..15632)
                     /gene="fdhF"
                     /locus_tag="ACHLAG_003124"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15213..15215),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFP0656304.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15830..17296)
                     /gene="mdtP"
                     /locus_tag="ACHLAG_003125"
     CDS             complement(15830..17296)
                     /gene="mdtP"
                     /locus_tag="ACHLAG_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFP0656305.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17293..19344)
                     /gene="mdtO"
                     /locus_tag="ACHLAG_003126"
     CDS             complement(17293..19344)
                     /gene="mdtO"
                     /locus_tag="ACHLAG_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFP0656306.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAKGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19344..20375)
                     /gene="mdtN"
                     /locus_tag="ACHLAG_003127"
     CDS             complement(19344..20375)
                     /gene="mdtN"
                     /locus_tag="ACHLAG_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFP0656307.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVGSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPFEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20394..20669)
                     /gene="ytcA"
                     /locus_tag="ACHLAG_003128"
     CDS             complement(20394..20669)
                     /gene="ytcA"
                     /locus_tag="ACHLAG_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFP0656308.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4707 a         5513 c         5697 g         4918 t
ORIGIN      
        1 tttgtttaat attatgggaa aaggtgatgc atgctggaga ggaagagtat tccccggtca
       61 ggcgaccggg gaaggggaga gattaatgtg cgcggccttg ctcaacgcca aagccggtct
      121 gggagcggat aaactgtgca cggaacagtt cacgctcgcg cgcaccttcc gctgagttat
      181 cggttgccga gaagaaccag atgccgagga atgccacgct gatagagaac agcgccgggt
      241 attcatacgg gaagatggct ttttcgtgac caaggatctg tacccaaatc gtcgggccga
      301 ggatcatcag taccactgcg gtaatcagcc ccagccagcc acccatcatc gcgccacgag
      361 tggtcagttt cgaccagtac atagaaagca gaatgatcgg gaagttacag ctcgctgcga
      421 tggcaaacgc caggcctacc atgaaggcga tgttctgatt ctcaaacagc acgccgagga
      481 taatggcaat cacacctaaa atcagcacgg tgattttcga tacccgcagt tcttcacgtt
      541 cggtcgcgcc ttttttgaag acgttagcgt acaggtcatg cgaaaccgct gatgcgcccg
      601 ccagcgtcag accagcaacc accgcaagaa tggtggcaaa agcaaccgct gaaataaaac
      661 cgaggaacag gttgccgccc actgcattcg ccaggtgaac ggccgccatg ttgttaccac
      721 caatcagatg gcccgccgcg tctttatatt ccggattcgc accaaccagc atgatcgcgc
      781 cgaagccgat aataaaggtc agaatataga agtagcccat aaacccggtg gcgtagaaca
      841 cgctcttacg tgcttcgcgg gcatcgctga ctgtaaagaa gcgcatcaga atgtgcggca
      901 agcccgccgt accaaacatc agtcccagac ccagagagag cgcggagatc ggatctttca
      961 ccagcccgcc cggcttcatg atgtcgacac ctttcgggtg taccgccatc gcttcactga
     1021 acagattgtt gaagctaaag ccgacgtgtt tcatcaccat aaaggccata aagctggcac
     1081 cgaacagcaa cagcacggct ttgataatct gtacccaagt ggtcgccagc atgccgccga
     1141 acaggacgta catcatcatc agcacgccga ccagcaccac cgcaatgtga tagttaaggc
     1201 caaacagcag ctcgatcagt ttacctgcgc ccaccatctg ggcgataagg taaagcgcca
     1261 ccaccaccag agaaccacag gccgaaagaa tacgaatcgg cccttgtttc agacggtaag
     1321 aggccacatc ggcaaaggtg tagcgcccca ggttacgcag acgttcggca atcaggaaca
     1381 aaatgatcgg ccagcccacc aggaagccca gcgagtaaat taagccgtca tagccggagg
     1441 taaacaccag cgcggagatc cccaagaatg aggcggcgga catatagtcc ccggcaatcg
     1501 ccagcccgtt ctggaagcca gtgatattgc cgcctgcggt gtagtagtcg ctacgagaac
     1561 gtacgcgttt tgatgcccag taggtaatgc cgagcgtaaa cacgacgaaa atcaggaaca
     1621 taataatcgc ctgccagttc gttggctggc gctctacggc cccgctaata gcatccgcgg
     1681 cgttagctgc gaaagggagt gtggcggcaa gcgccgtcag aactcttttc atgatgcttg
     1741 tacctcatgc aggacttcat tattaagacg gtcgaattcg ccgttcgccc gccagatgta
     1801 gataccggtg agaacaaagg agatcacaat cactccaaca ccaattggaa taccgcgtgt
     1861 gacgctggtg ttcggattca gcggcgtgcc cagccagccg ggcgcgaagg cgatcagtaa
     1921 aataaagccg atataaattg ccagcataat aatcgacagg atggtggcaa accgttgccg
     1981 tttttcgact aactccctga aatgcgcatt gtcttctatc cgctgataaa tagtgccatt
     2041 catcacagat tctccagagg taatgtaggg attgttttaa ttcccgctcc cttatgggag
     2101 aaggttaacg ctcgggtaac ccttgccgaa tgtaggccgg ataaggcgtt tacgccgcat
     2161 ccggcaatca atgcctgatg cgacgctgtc gcgtcttatc aggcctacaa accgttaccg
     2221 actcgcatcc ggcaattgtg ggttacgatg gcatcgcgat agcctgcttc tcttcaagca
     2281 gcttctcgac tacgccagga tcggcaagcg tcgaggtatc gcccaggttg ctggtatcgc
     2341 ccgccgcaat tttgcgcaga atacggcgca taattttgcc ggagcgggtt ttaggcaggg
     2401 agtcggtcca gtgcagcacg tctggcgtcg ccagcgggcc aatctcttta cgcacccagt
     2461 tgcggacttc tgcgtacagt tctggtgacg gttcctcccc gtgattaagc gtgacgtagg
     2521 cgtagatcgc ctgaccttta atattgtgcg gaatacctac tacggcggct tcggcaatct
     2581 tcggatgcgc caccagcgcc gactcaatct ctgccgtccc cagacggtga ccggagacgt
     2641 tcagcacgtc gtccacacgc ccggtgatcc agtaatagcc atcttcatcg cgacgcgcgc
     2701 cgtcgccgct gaaatacata tttttgaagg tggagaagta ggtctgttca aaacgttcgt
     2761 gatcgccaaa cagcgtacgc gcctgacccg gccaggagtc ggtgatcacc aggctgcctt
     2821 cggtagcgcc ttccagcggg ttaccttcgt tatcgaccag cgccggttgc acgccgaaga
     2881 acggacgtgt tgccgaaccg gctttcagct cggtagcgcc aggcagcggc gtgatcatga
     2941 aaccgccagt ttcggtctgc caccaggtat cgaccaccgg acatttctcg ttgccgattt
     3001 ttttccagta ccactcccac gcttccgggt taattggctc gcccacggaa ccgagaatgc
     3061 gcagcgacga acggtcggta ccctcgatcg ctttatcgcc ttccgccatc agcgcgcgga
     3121 tcgccgtggg tgcggtatag agaatattga cctgatgctt gtccaccacc tgcgccatac
     3181 gggcaggcgt cggccagttg ggtacgcctt caaacatcag cgtggtcgca ccgcaggcca
     3241 gcgggccgta cagcaagtaa ctgtgtccgg tcacccagcc cacatcggcg gtgcaccagt
     3301 agatatcacc cggatgataa tcaaagacat atttaaaggt cagcgccgcg tacaccagat
     3361 aaccgccggt agtatgcagc acaccttttg gcttaccggt agaaccggag gtgtagagaa
     3421 taaacagcgg atcttcggcg ttcatctctt ccgcctggtg ctgatcgctc gcttgctcaa
     3481 ccaggtcgtg ccaccacagg tcgcgccctt cctgccagtc aattttcccg ccagtacgct
     3541 tcagtaccac cacatgctct acgctggtga cgttcgggtt tttcagcgcg tcatcaacgt
     3601 ttttcttcag cggaatactg cgcccggcac gcacaccttc gtcggaagtg atcaccagtc
     3661 gtgagttgga atcaataatg cgcccggcaa cggcttccgg cgagaagccg ccgaaaatca
     3721 ccgaatgcac cgcgccaatg cgggcgcagg ccagcatcgc aaccgcggct tccggcacca
     3781 tcggcatata aatcgccacc acatcacctt ttttaatgcc cagctcgagc agggtattgg
     3841 cgaagcggca gacgtcgcgg tgcagctctt tatagctgat atgtttgctc tggctggcgt
     3901 cgtcgccttc ccagatgatg gcggtacgat cgccgttttc ttgcagatgg cggtcaaggc
     3961 agtttgccgc cagattcagc gtgccgtcct cgtaccattt aatggacaca ttaccggggg
     4021 caaaggaggt gtttttcacc ttctggtaag gtttgatcca gtcaagaatt tttccctgtt
     4081 cgccccagaa ggtatcaggt acgttaatag attgttgata catcgcctcg tactgctgag
     4141 ggtttatcag gcaacggtct gcgatgttgg caggaatggt gtgtttgtga atttggctca
     4201 tgcttttgtt ctccttgtag gatgttaata atatgtggca taagcgttaa atgtaggggt
     4261 attggcagtt ttgtttagta tttgggcgac agatcacgca aaagtagaat tgtgcaaata
     4321 aacggcaggg taatttttga aggtcagcaa caaaagttga ttaattcttt gaggaacatg
     4381 cagttatgca tgctgttgaa aaagaggaag atactgacta actctaaagt ggtattttac
     4441 atgcacttac aattgattaa agacaacatt cacagtgtgg ttatttgtta cacatagggg
     4501 cgagcaatgt catgacagtg taggtgcggt tactgtcgtg aaaaacaata aaaaccgcca
     4561 ttgcaacaat ggcgcaattc ggatgaagcc cctatgacaa ggataaaaat aaacgcacgc
     4621 cgtatcttca gcttattgat tccttttttc tttttcactt ctgttcacgc tgaacaaacg
     4681 gctgctcccg caaaacctgt aactgtggaa gcgaagaatg aaacctttgc cccgcagcat
     4741 cccgatcaat atctctcctg gaaagccacc tcggaacagt cagagcgtgt tgacgccctg
     4801 gcggaagatc cacggctggt gatcctgtgg gcggggtatc ccttctcgcg cgattacaac
     4861 aagccgcgtg gacatgcctt tgctgtgacc gatgtgcgtg aaaccctgcg taccggtgcg
     4921 ccgaaaaacg ctgaagatgg tccgctaccg atggcatgct ggagttgtaa aagcccggat
     4981 gtggcgcgtc tgatccagaa agacggcgaa gatggctact tccacggtaa atgggcgcgc
     5041 ggcggcccgg aaatcgtcaa caacttaggt tgtgccgact gccataacac cgcctcacca
     5101 gagttcgcca aaggcaaacc ggagttaacc ctttcccgtc cgtatgcggc tcgcgcgatg
     5161 gaagccattg gtaaaccttt tgagaaagcc ggacgtttcg accagcaatc gatggtttgc
     5221 ggtcagtgcc atgtggagta ttacttcgac ggcaaaaaca aagcggttaa attcccgtgg
     5281 gatgacggca tgaaagtcga aaatatggag cagtattacg acaaaattgc cttctctgac
     5341 tggactaact ccctgtcgaa aacgccaatg ctgaaagcgc agcacccgga atatgaaacc
     5401 tggacagcgg gcattcacgg taaaaacaac gtgacctgta tcgactgcca tatgccaaaa
     5461 gtgcagaacg ccgaaggcaa actctacacc gaccataaaa ttggtaatcc gtttgataac
     5521 ttcgcccaga cttgtgcgaa ctgccatacc caggacaaag ctgccttgca aaaagtggtc
     5581 gcggaacgta agcagtcgat taacgacctg aaaatcaagg ttgaagatca actggttcac
     5641 gctcacttcg aagcgaaagc agcgctggat gcaggcgcga cggaagccga aatgaagcca
     5701 attcaggacg atatccgtca tgcccagtgg cgctgggatc tggcgatcgc ttcccacggc
     5761 attcatatgc acgcaccgga agaaggttta cggatgctcg gtacggcgat ggataaagcg
     5821 gcggatgcac gcaccaaact ggcgcgcctg ctggcgacca aaggcatcac ccatgaaatc
     5881 cagatcccgg atatctcaac caaagagaaa gcccagcagg ctattggcct gaacatggaa
     5941 caaatcaagg ccgagaagca ggacttcatc aaaacggtga tcccgcagtg ggaagaactg
     6001 gcacgtaaaa acggtctgtt aagccaataa ccccgttccg cctcgcaagg ggcggaaaac
     6061 acaatggagt gaatatgagc gtattacgtt cgttattaac tgccggggtg ctggcgtcgg
     6121 gcctgttgtg gagcctgaac ggaattaccg ctacccctgc ggcgcaggca tctgatgatc
     6181 gctacgaagt tacccagcag cgtaacccgg atgccgcctg tctggactgt cataagccag
     6241 ataccgaagg tatgcatgga aaacatgctt ccgttatcaa cccgaataac aaactgccgg
     6301 tcacctgcac caactgtcac ggccagccat caccgcaaca ccgcgaaggg gtgaaagatg
     6361 tgatgcgctt taacgagccg atgtacaagg ttggagagca gaacagcgtc tgtatgtcct
     6421 gtcatctgcc ggaacagttg caaaaagcgt tctggccgca cgatgtccac gtaaccaaag
     6481 tggcgtgcgc cagctgccat tccctgcatc cacagcaaga tacgatgcag acgttaagtg
     6541 acaaaggacg gattaagatt tgcgtcgatt gccacagcga tcagcgcacc aatccgaact
     6601 ttaacccggc gtctgttccg ttgcttaagg agcagccatg acctggtctc gtcgccagtt
     6661 tctcaccggc gtcggcgtgc tggcagccgt cagcggcacc gcagggcgcg tggtggcgaa
     6721 aacgttgaat atcaatgggg tgcgttacgg catggtgcat gacgagtcgt tatgcatcgg
     6781 ctgtaccgcc tgtatggatg cttgtcggga agtgaacaag gtgccggaag gcgtctcgcg
     6841 cctgacgatt attcgtagcg agccgcaggg cgaatttcct gatgtgaaat atcgcttctt
     6901 ccgtaagtct tgccagcact gcgatcatgc gccgtgcgtt gacgtctgcc cgaccggtgc
     6961 gtcttttcgt gatgccgcta gcgggattgt cgatgttaat cctgatctct gcgtcggttg
     7021 tcagtactgc atcgccgcct gtccgtaccg cgtgcgcttt atccatccgg tcacgaagac
     7081 ggcggacaaa tgcgatttct gccgtaaaac caatttgcag gccggtaagc tgcccgcgtg
     7141 cgttgaggct tgcccgacga aggcgctgac gtttggcaat ctggacgatc ctaacagtga
     7201 gatttcgcaa ctgctgcgcc agaagcctac ttaccgctac aagctggcgc tgggaaccaa
     7261 accgaagctg taccgcgtac cgtttaaata cggggaggtg agccaatgac gcagacttcc
     7321 gcatttcatt ttgaatcgct ggtgtgggac tggccgattg ccatctacct gtttttgatt
     7381 ggtatttctg ccggtctggt gacgctggcc gtgctgttac gtcgcttcta cccgcaggcg
     7441 ggcggtgcag acagtacgtt gctgcgcacc acgctgattg tcgggccggg cgcggtgatc
     7501 ctcggtctgt tgatcctcgt tttccacctg acaagaccgt ggaccttctg gaagctgatg
     7561 ttccactaca gttttacctc ggtgatgtcg atgggggtga tgctgtttca gctctacatg
     7621 gtggtgctgg tgctgtggct ggcgaaaatc tttgaacatg atttgcttgc cctgcaacaa
     7681 cgctggttgc cgaagctggg gatcgtgcaa aaggttctga gcctgctgac gcccgttcat
     7741 cgcggactgg aaacattgat gctggtgttg gcggtgttgt tgggggctta taccggcttt
     7801 ctgctgtcgg cgctgaaatc gtatccgttc ctcaataacc cgatcctgcc ggtgctgttc
     7861 ctcttctccg gcatctcgtc cggtgcggcg gtggcgctga tcgccatggc gatacgccaa
     7921 cgcagtaacc cgcattccac ggaagcgcag tttgtacacc gtatggaaat ccccgtggta
     7981 tggggtgaaa tcttcctgct ggtggcgttt tttgtcggtc tggcgctggg cgatgacggt
     8041 aaagtgcgtg cgctggtggc ggcattaggt ggcggtttct ggacgtggtg gttctggctt
     8101 ggtgtcgccg ggctggggct gattgtgcca atgttgctca aaccgtgggt caatcgcagt
     8161 tccggcattc ctgccgtgct ggcggcgtgt ggggccagtc tggtcggcgt gttgatgctg
     8221 cgctttttca ttctctacgc cggacagttg acggtggcgt aagccagaaa agaggtggtt
     8281 tctggacgta ttccttcctg aagtcggttt tctggcgttg ttgttaagtc tcggggtcaa
     8341 cgtgttgacc ccgttgacgg cctttgcggg agtgcggttg cgctggcctg ccatgatgcg
     8401 actcacttgc atcggcattc tggcgcagtt cgcgctcctg ctgctcgcct ttggcgtact
     8461 gacgtattgt tttctcatca gcgatttctc ggtcatttat gtcgcgcaac atagctatag
     8521 cctgctgtcg tgggaactca agctggcagc ggtgtggggc ggccatgaag gttcgctgct
     8581 gctttgggtg ctgctgcttt ccgcctggag cgcgctgttt gcctggcatt atcggcagca
     8641 aaccgatccg ctatttccgc tgacgttagc cgttttatct ctcatgctcg ccgcactgct
     8701 actgtttgtg gtgctgtggt ccgatccctt cgtgcggata tttccaccag caatcgaagg
     8761 ccgcgatctc aatccgatgc tgcaacatcc cggtcttatc tttcatccac cgctgcttta
     8821 ccttggctat ggcggtttga tggtagcggc gagcgtggcg ctggcgagtt tactgcgcgg
     8881 cgagtttgat ggtgcctgcg ccagaatttg ttggcgctgg gcactacctg gctggagcgc
     8941 attaacggcg gggatcatcc tcggttcctg gtgggcctat tgcgaactgg gctggggcgg
     9001 ctggtggttc tgggatccgg tggaaaacgc ctctttatta ccctggcttt ctgccactgc
     9061 gctgctgcac agtttgtccc tgacacgcca gcgggagatt ttccgccact ggtcgctgtt
     9121 gctggcgata gtaactctga tgctgtcgct actgggtacc ttaattgtcc gttctggcat
     9181 tctggtttcg gttcatgcgt tcgcactgga taacgtccgc gccgtgccgt tgttcagcct
     9241 gtttgcactg attagccttg cgtctctggc tctgtatggc tggcgagcgc gggacggtgg
     9301 cccggtggtg cgtttttcgg ggttatcgcg ggaaatgtta atcctcgcta cgctgttgct
     9361 gttttgcgca gtgctactga tcgtgctggt gggaacgctt tatccgatga tttacggtct
     9421 gctgggctgg ggacgcctct ccgttggcgc gccgtatttt aaccgcgcga cgttaccgtt
     9481 tggcctgttg atgctggtgg tgattgtgct ggcgacgttt gtctctggca aacgcgtgca
     9541 gcttccggcg ctggtagctc atgcaggcgt gctgttattt gccgcaggga tcgtggtttc
     9601 cagcgtcagc cgtcaggaga tcagcctgaa tttacagccg ggtcagcagg tgacgctggc
     9661 aggatacacc ttccattttg agcgcctcga tctgcaagcc aaaggcaatt acaccagcga
     9721 aaaagcgata gtggcactgt ttgaccatca gcaacgcatt ggtgaactga tgccggagcg
     9781 gcgtttttac gaagcacgtc gtcagcaaat gatggaaccg tcaattcgct ggaacggcat
     9841 ccatgactgg tatgcggtca tgggtgaaaa aaccggagcg gatcgttacg cttttcgttt
     9901 gtatgtacaa agcggtgtgc gctggatctg ggggggagga ttgttgatga ttgcgggcgc
     9961 attgttaagc ggatggcggg ggaagaagcg cgatgaataa agggcttctc acgctactgc
    10021 tgttatttac ctgttttgcg cgcgctcagg tcgtagacac ctggcaattc gccaatccgc
    10081 aacaacagca acaggcgtta aatattgcca gccagttacg ttgtccgcag tgccagaatc
    10141 aaaacttact ggaatccaac gcgccggtgg cagtcagtat gcgccatcag gtttacagca
    10201 tggtggcgga ggggaagaac gaagtcgaaa tcattggctg gatgaccgaa cgctacggag
    10261 attttgttcg ctataaccca ccgttaacgg gtcagacgct ggtgttgtgg gcgctgccag
    10321 tggtgttgtt actgctgatg gcactgatcc tctggcgagt gagggcgaag cgatgaaaca
    10381 gcccaaaata ccggtgaaaa tgctgacaac actcacgata ttgatggtat ttctctgtgt
    10441 cggcagttat ctgttaagcc caaaatggca ggcggtacgt gcggagtatc agcgtcagcg
    10501 cgatccgcta catcagtttg ccagcaagca aaccccggaa gcgcagcttc aggcattaca
    10561 ggataaaatc cgcgctaatc cacaaaacag cgaacaatgg gcgttactgg gcgagtacta
    10621 tctgtggcaa aacgattaca gcaattcgct gctggcgtac cgtcaggcgt tgcaactacg
    10681 tggtgagaac gctgaactgt atgcggcgct ggcgacggtg ctttattacc aggccagcca
    10741 gcatatgacc gcccagactc gcgcaatgat cgacaaagcc ctcgcgctgg actctaacga
    10801 aatcaccgct ctgatgctgc tggcttccga tgcgtttatg caggcgaatt acgcgcaagc
    10861 catcgaacta tggcaaaaag tgatggatct caactcaccc cggatcaacc ggacacagct
    10921 ggtcgagtcg attaatatgg cgaaattgtt gcagcggaga tcagattaat catctggtga
    10981 tgaagattat gttattgagt tgtataaaaa ataatcgaat gaacatatgc caaaaataat
    11041 caccaatcag tattattgca gattaaacaa ataaaaatct ttccataaca aatggttatt
    11101 cattaatcct gccataaact cgcgttatta tgcattaatg cagcgaaaag ctctgttgtt
    11161 aaagggttgc gcaacatacc gcgcaaatga tactgatcat aagcgttaaa aaaatctaca
    11221 aaccaacgca acacaattca tgccctggca gtatgtcacg ttctcgcgtt tctgaacggg
    11281 gaacggcgct ccattgagga agttattctg atgaaaaata taaaattcag cctggcctgg
    11341 cagattctgt ttgctatggt gctgggcatt ctcctgggaa gctacctgca ctaccatagc
    11401 gacagccgcg actggctggt cgtcaatttg ctctctccgg cgggtgatat cttcatccat
    11461 ctgattaaaa tgattgttgt gccgattgtg atctccacgc tggtggtggg tatcgcgggt
    11521 gtgggtgatg ccaaacagct cgggcgtatt ggcgcgaaaa ccattattta cttcgaggtg
    11581 atcaccaccg tcgccatcat tttggggatc actctggcga acgtcttcca gcccggtgcc
    11641 ggggtcgata tgtcgcagct ggcgaccgtc gatatctcga aatatcagag cactacggaa
    11701 gcggtacaaa gcagttccca cggcattatg ggcacgattt tgtcgctggt gccgacgaac
    11761 attgtggcgt cgatggcgaa aggcgaaatg ctgccgatca tcttcttctc ggtgctgttt
    11821 ggtctggggc tttcctccct gcccgcgacg catcgtgaac cgctggtgac cgtgttccgc
    11881 tccatctctg aaaccatgtt taaagtgact cacatggtga tgcgttatgc accggtgggt
    11941 gtgtttgcgc tgattgcggt gacggtggct aactttggtt tctcttctct gtggccactg
    12001 gcgaaactgg tgctgctggt gcatttcgcc attctgttct tcgcgctggt agtgctggga
    12061 attgtggcgc gcctgtgcgg gttaagcgtc tggatcctga ttcgtattct gaaagatgag
    12121 ctgattctgg cgtactccac tgccagctct gaaagcgtac tgccgcgaat tattgagaag
    12181 atggaagcct acggagcacc ggcgtcgatc accagcttcg tggtgccgac cggttactct
    12241 tttaaccttg atggttcgac gctgtatcaa agtattgccg ctattttcat cgctcagctg
    12301 tatggcattg acctgtccat ctggcaggaa atcattctgg tgctgacgct gatggtgacc
    12361 tcgaaaggga ttgctggcgt gccgggcgtg tcgtttgtgg tgctgctggc aacgctgggt
    12421 agcgtaggta ttccgctgga aggcctggcg tttattgctg gtgttgaccg tatcctcgac
    12481 atggcgcgta ctgcgctgaa cgtggtgggt aatgcgctgg cggtgctggt gattgccaag
    12541 tgggaacaca aatttgaccg caaaaaggcg ctggcttatg agcgtgaagt gctgggcaaa
    12601 tttgataaaa ctgcggatca ataattgaag attgccgggg ttatccaccc ggcaatgtgt
    12661 ggatgcctga tgcgacgctg gtgcgtctta tcaggcctac cttaaccgtt ggttaatttt
    12721 tcaaactctt cacacccggt atcaaaccct tccatacagc tcaggttcaa ccagtgcagc
    12781 gccttttgtt tgttcttctc gataaaacct tcctcaccgt ttaagaacat cattcccgcc
    12841 cagtactcgg aataaccggt tcgggaaatt gcagagctgc gtttgaaata ccaggttgcc
    12901 ttgtcatcgt cggccttaat gcccacgccg ttggcgtaaa tcaaaccaag cagcatttgg
    12961 gcatcgacgg cggagtcatt ctccagatct tccgaggcgt tttctaacag cgaaatcgcc
    13021 tttggataat ccggtttacc cgcttgagta tttaccagaa tatgcgccag cgttacttca
    13081 ccttctttac tgcccgcttg cgccgctttc tctgcaagaa cttttgcttg tggatagtcc
    13141 agactgaccg gattggtgat tttgatctgc gccagcagcg cgcaggcatc agcatcaccg
    13201 ctgtcggcgg ctttctgtgc ccaatattcg gctttgctca aatcgccgga gctaaaccag
    13261 ctgtcggcaa gaaaatattg tgcgcgtcgg tccccggcct ctgctgcctt taaatactgg
    13321 ctgccaggct cggagtcgtt cgcgtgggca aagaatgtca aaaacaacat caatgcgata
    13381 atttttttca ttttgataat acgattgggt agggagtaac cagtataaag agttcgaatg
    13441 gataaaaaaa cagcctccga aaggaggctg tagaaaggac tatattacgc cagtgccgct
    13501 tcgcgcaggc gagttttcaa cttgttgtac tcgtcaatca cgtactgctc ggcggcgcgc
    13561 tgatcggcga tcggctcgac gcgcacggcg cagtatttgt attccggcgt tttcgtaatc
    13621 gggcttaagt tttcggtaac cagctcgtta caggcaccaa tccaccactg gtaggtcatg
    13681 taaatcgccc ctttgttcgg acgatcgctg acctgcgcac gggtgataat tttgccttta
    13741 cgcgagtgca cccaaaccaa tgcctcatct tcaataccca gacgtttggc gtcttcggta
    13801 ttgatttgtg cgtagccagg ttcatcagcc agcgccgcca gtgccgcaca gttaccggtc
    13861 atcgaacggc aagagtagtg accaacttca cgcaccgttg acagtaccat cgggtactcg
    13921 tcggtgagtt tgtcgattgg cgctacccag tcgcaggtga agaactgtgc cagaccgttc
    13981 ggggtatcaa acttctcttt aaacagataa gaagtcccct gatcggcatc tgaagtatcg
    14041 cggcaaggcc actgaatgaa gcccagttcg cccattttct cgtaagtcgc accgtagaaa
    14101 tccgggcaca gatgacgcaa ctcatcccag atctcctggg tgttgttgta gtgcatcgga
    14161 taacccatac gggtggcgat ttcactgatg atttgccagt ccgttttcag atcccatttc
    14221 ggttcaactg ctttgaagaa acgctggaag ccacggtcag ccgcagtaaa cacgccttca
    14281 tgctcgcccc acgacgttga cggcaaaatg acatccgccg ccgacgcggt tttggtcata
    14341 aagatgtcct gaacgataac cagttccaga tcttcaaagg ctttacgtac tgccgacagc
    14401 tccgcgtcag tttgcagcgg atcttcgccc ataatgtacg cggcacgcac ttcgccatgc
    14461 gctgcgcggt gcggcagctc gctgatgcga taaccggtat gagcaggcag gctttccacg
    14521 ccccaggctt tggcgaattt ctcgcggtta gccggatctt tcacgtactg gtatcccgga
    14581 tacgtatccg gcagcgcgcc catatcgcag gcaccctgca cgttgttctg accacgaacc
    14641 gggtttacac ccgcatgcgg cttaccgagg ttaccggtca gcatcgcgag gctggtcaga
    14701 gaacgcacgg tttccacgcc ctggtagaac tgggttacac ccatgcccca caggatggcg
    14761 gcgcttttcg cctgggcata catccgtgcc gcctgacgaa tctcactggc gctgacgccg
    14821 gtgatatctt caaccgactc cggcgtgtag ccttcaacga ttttacgata ctcttcaaag
    14881 ccttctgtac gggaagcgac gaacgctttg tcgtacagat tttcttcaat aatgacatgg
    14941 cccatcgcat tcaacagcgc gatgttcgag ccgtttttca gtgcaatgtg catgtcagca
    15001 atgcgcgcgg tttcaatttt gcgcggatcg cagacgataa ttttcgcccc gttacgttta
    15061 gcgttaatta cgtgattcgc cacgattggg tgggaatccg ccgggttgta cccgaaaacg
    15121 aacactaaat cggtattatc aatttcgtta atagcattgc tcattgcgcc attaccgacc
    15181 gattggtgca gacctgcaac cgatgggccg tgtcagacac gagcgcagca gtcaacgtta
    15241 ttggtaccaa taacggcgcg cgcaaatttt tgcattacat agttggtttc gttacccgta
    15301 ccacgcgagg agccggtcgt ctggatggca tccggaccgt acttctcttt gatggcgctc
    15361 aggcgctcgg caacgtaatt cagtgcctca tcccaggaaa caggttcgag tttgccgcca
    15421 cgctggcgac ggatcatggg ggttttcagg cgcggggtca ggatctgggt atcgttaatg
    15481 aaatcccagc cataataacc cttcagacac agggtaccct ggttggtttt cccctgcgct
    15541 gcctccgccc ggacgatttt gccgttatcg acgaccaggt tgattttgca acctgatgcg
    15601 caataggggc aaaccgtgac gacttttttc atcggtctcg ctccagttaa tcaaatcacg
    15661 catacgcgct ctcgactaca gtatgcatct tttatgccac attttatttg gggtcattcc
    15721 ctgatattac gggcactatt tattcaaaac tctgacgaaa aacaggctgt cgtcagtttt
    15781 gacgtgacga aacgaaatac cgcgtgacag ccatcacgcg gcagacattt tattttttct
    15841 cgacgacggg agccgcctga tacccgccgc ccagcgattt catcaactga atgctttgga
    15901 tcacccggcg gctgtccagc atcagtaatg acatctcttc ggcaagtact ggcaaccggg
    15961 cttcagtggc ctgtaagcgg ctggttaagc cgcgctgata ggcggcctcg gcagcgcgct
    16021 gggtgaagcg cgttgcttcc acgcgttcag cctgcatttc tcgctcgtcg ttgagcgttt
    16081 gcagacgcgt gccgttgacg gcaacgtcac gcaccgcgtt cagtactgac tggttgtaac
    16141 gttcaatcat catgttgctg gcggcgcgcg tgccttcgag attggcattc aaccgtccac
    16201 cgtcaaacag cggcaatttc agacccggga tgaagttgaa ctggcgactg gtttttttga
    16261 ataaggtatc caggtggatg gcatcaaggc cgaaaaacgc tttgatatca aagctcgggt
    16321 agaacagcgc ccgcgcggaa tccacctgat ctaatgacgc ctgaacatac cagcgcatgg
    16381 cttgcagatc cgggcgtctg gcgagcaact cataagagag tgtcgccgga atgccggtct
    16441 ggactcgtgg taatgccacc ggtttgatct ccggcatatc gctggccccc gcgccaatca
    16501 atgcacgcag agattctcgc gtttcggtga tttgcccttt gacggcagca atttgtttat
    16561 cgaccgccag aatctgtgcc cgcgcgccgt ggaaaggcac ttgtgcttcc agaccgtgcg
    16621 ccactttact ctggtgcgct ttcaccgcgt aatcaatcac atcgcgagtt tgttctaaca
    16681 gatcgagcat ctgatagctg gcctgcatac tgtaataaag ctgcgctacg cccgtggtca
    16741 gcgatagctc tactgctgcg gtttctgcca gcgcggcatt atgcgcgcca atggcggcgg
    16801 caaccgctga gcgatgcaca ccccacaaat caagatccag tccggcaaac aaacctactg
    16861 tggcttccgt atagtacggc ccgtccatac ccagtgcggg cgcatccatc gcataagggc
    16921 ttaaaaagcc gttcgccgag acgcgttggc ggttgagcat ccctaacgct gcgacctgta
    16981 attgtgaacc ggcatctaac aaatcggcct gggactgcgc tttttcttcc cgcagtttcg
    17041 cttcggcgag ggtgtgtgaa ccacttagcg tccgttggat cagcgcatcc agctgcgggt
    17101 cattgagttg tttccaccac tgcgcctgcg gccagccgga gctggcaaga tgaatatcgt
    17161 cggccagttt gatttgttcc ggtttgagct gttgatgagg tgcagaatcc ttacgtacca
    17221 gggcacagcc ggaaatcagc gtcgtgctgc cgagaatgct gcacaacagc agacgtgaaa
    17281 gttgacgatt gatcattgcg tggctccttg tgcctgttcc gtggcgggcg ttaatgccgg
    17341 ggctgtccag tctggcaagc gggcgaaaag ctggcagacg tgttgctcct gttttaataa
    17401 ggtgggcagg atggcctgcg aggcgggtgt ctcttcaagc gttatttgag gagaacggct
    17461 gagtgcggtt gccagaccgg cagcgtattt ctccagggcg tcggcaaact gagctgctct
    17521 gtccggctgt agtgcgttat ccagcgcctg cgccgagttc caggtggcat cccaggcgtg
    17581 aagaatatcg cggccctgac gaataaccgt ttgcgaacgt tcaatcagta aggcgcgttc
    17641 ttcgctgtcc agttgacgct ccagcgccac gcgttggcac atctcttcac aggcattaaa
    17701 cgccgcatgc agaccgatac gaatttgcag ataagtgcgc agagccgtga cttcctgctg
    17761 gcgtgggatc cgcattactt tacttaacat acccagcgtg ccagccagtt tttgcggcag
    17821 tgtgcgcgct tcactttcag gccagacaaa ggtgtaaatc accgcggaca ccacggtacc
    17881 aatgatgata cccatggcgc gatcgcgaat ttccaccagg tcgtacaccg ggccaaaaac
    17941 gttttcgagc gtggcgagcg cgaaggtgac caccatctgt gtgccgatat aagaagagcg
    18001 ttcagagctg gtggcaatcc atgcgcccaa caggaaaatc ggtgccagca caaacagcaa
    18061 ttcgacaata ttgtccagcc agggcatgac cagtagcgtg aataacagcg ccagaatcgc
    18121 gccgcaaaag gccccgccaa aacgcagcac catcttctgg tacgacgaac cgacatttgg
    18181 gttagcgacg atcacgcatg tcagcataca ggtgtgaatg ccttcccagt ccacgccgct
    18241 gtagaaggtg taacagatca aacaggcgag cagtgttttt accgcgtagc gcatatagtc
    18301 tggattggta aaagcatcgg cgaccattga tggcggtttg gcggcgggcg ttggcggcgt
    18361 attcgggtcc atctggccca gttgtaacaa cgtctggcag atattctcca ggttacattc
    18421 ccgtgccgcc atcgcttcac tttcagtgat ccgccagtcg ctttgccagc actgaccttt
    18481 agcaacggca tgctgcagct tattgatttc tgaggctaat ttttgccgaa attcaataat
    18541 tgcctgagaa tcagcaaaag aggtgggatc gtagcgattc agcgtcgagt aaatgtaggt
    18601 taccgttgcc acgcagcttt gccaccatgc gctttgagtt cgccagttgg catcgtccgc
    18661 gagacaaaag acattcagtt tttgcagcgc cagcgcctct ctttcaatcc gcgtttcggg
    18721 tagcggtgcg aggctgtccg tcaggtgact aatggcatca tcaagccgat cattaagcgc
    18781 ttgatgcatt tgcgtaatgg cacgactggg aaaccacagc acgccgatta acgtcatcag
    18841 caaggttgga tagaggccaa caacgataca ccacagcgtt aagcgcacga ccacttccgg
    18901 atagtcgagc atggcgggga aggtttgccc gtaaatggcg acaatggcga cagcgaaaaa
    18961 gaccagtccc agccgatggg tgcgcatcaa aaacatacag cccatcagga tcggcccggc
    19021 gatgatcaat cggatcaacg gttcgccgta tgaccatttg tagatcaaaa acaggctgcc
    19081 gatctccagc accgtggcaa ccacaaacaa gatcgcgaca aatttggtgt aaaacgcgtt
    19141 cgactgaata ccgtaaaaca gcactgccag cgataacgcc acaaaaggga tctcaaaagt
    19201 catcgagatc agaatcacca gcaggcagcc tacccagagt tgcacgatct gcggcacgcg
    19261 gccgggtcgc cgctcgctta actcttcatg aaagaacgcc agcagcctga ccaccggtaa
    19321 tggcagggag ttgagcgcgc tcattattgc ggctcaagat tagcgactgc cgaagcgccg
    19381 atgcggaaca tttccgggtc aggtttatcg accatgattt tgaccggaaa acgctgggca
    19441 acgcggaccc agttaataga gcgagaaact ttcggcaggc cgcccagcac caggccgccg
    19501 tcatccggta gcacgccgta gccaatcgaa tccactttac cctcgaaggt tttgccgctg
    19561 tcactcatca ggcgaatcgt tgcgggtgta cctgagcgaa tatttttcag atcggtttcg
    19621 cggaagttgg cgatcacgta ccagtgacga gtgtcgatta gggtaaaaat agggcgcatg
    19681 gcagaagcaa attgcccgac ggaggttttg agggaaatga cccggccatc aaacggcgcg
    19741 cgaacggtcg ccatttccag atgaagtttg gtcagggcaa tatccgcttc gaccgccgca
    19801 cgttgcgcca ccagcgcatc gacgccgctg acggcgctgg cggctgactg cgcctgtaac
    19861 aatacggcat taagatcggc ttctgcggcg cgctgcgcag ttcttgcacg gtcaacgtct
    19921 tccgctgaga caaaaccctc tttcagtaat ggctcggtgc ggcgtaatgt atctgtggcc
    19981 tgtttcgcgg cggcacgggc tttttctacc gtggcattaa tcgagtcggc accaaactgt
    20041 tgcgcatcaa cgctacgctg ggtgagcata atttgcttat ccagcgccgc gagggaggct
    20101 tcagctttcg ccagattggc ttcgaacggg cgcgggtcaa tgcggaacag caaatcgccc
    20161 tgtttgactg cctggttgtc ggtgaccgcc agttctacaa tgcggccgct gacttccggc
    20221 accacatcaa tggtatctgc tgacgcgtaa gcgtcattag ttgatggcgc actgcctacg
    20281 cgccagataa cgaaaacaag ggcaaccagc gccaatgcaa ccactaacag agcagggaat
    20341 ttactgcgag gagctttttt cggcgtactt tccatgagcg cgtctcaaat agattagaaa
    20401 aatgccagcc agaacagcat ggcgtagaga gcaaaaaggg cggtataaat aattccgaca
    20461 aatgccagat tgatatttgc ccgctgaata actcgcctcg tgatgagcat caaaataagg
    20521 ctggcaatgg cgcagaaaaa ccagctggga taataagcac cgatcaccgg gattgccgga
    20581 gataatgagc atcccgaaac catgaagacg ggaattatcc aggctgtttt tttgagttgc
    20641 atcgccatgc gagagagaac tgttggcata ttaaatacat cctgtctgct gcgaacaata
    20701 tctgtagtga atgtcaatat ccattgaagg cgtcagtatg gctattttct cgatgaataa
    20761 aaatagcttt attcagccac cgcattcata acttatatat tttatttatt aattggctgt
    20821 atatattttt aaaat
//
LOCUS       DAZGVQ010000089        20695 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000089 DAZGVQ010000000
VERSION     DAZGVQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.090"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            67..1449
                     /gene="dosC"
                     /locus_tag="ACHLAG_003129"
     CDS             67..1449
                     /gene="dosC"
                     /locus_tag="ACHLAG_003129"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFP0656309.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            1474..3873
                     /gene="dosP"
                     /locus_tag="ACHLAG_003130"
                     /gene_synonym="pdeO"
     CDS             1474..3873
                     /gene="dosP"
                     /locus_tag="ACHLAG_003130"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFP0656310.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENNEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            4131..4712
                     /gene="ddpX"
                     /locus_tag="ACHLAG_003131"
     CDS             4131..4712
                     /gene="ddpX"
                     /locus_tag="ACHLAG_003131"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFP0656311.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIY
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            4726..6276
                     /locus_tag="ACHLAG_003132"
     CDS             4726..6276
                     /locus_tag="ACHLAG_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP0656312.1"
                     /translation="MKRSIPFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            6278..7300
                     /locus_tag="ACHLAG_003133"
     CDS             6278..7300
                     /locus_tag="ACHLAG_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0656313.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            7297..8193
                     /gene="ddpC"
                     /locus_tag="ACHLAG_003134"
     CDS             7297..8193
                     /gene="ddpC"
                     /locus_tag="ACHLAG_003134"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFP0656314.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            8190..9176
                     /locus_tag="ACHLAG_003135"
     CDS             8190..9176
                     /locus_tag="ACHLAG_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0656315.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTLRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            9169..10095
                     /locus_tag="ACHLAG_003136"
     CDS             9169..10095
                     /locus_tag="ACHLAG_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP0656316.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGEAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(10151..10582)
                     /gene="osmC"
                     /locus_tag="ACHLAG_003137"
     CDS             complement(10151..10582)
                     /gene="osmC"
                     /locus_tag="ACHLAG_003137"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFP0656317.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            10928..11143
                     /gene="bdm"
                     /locus_tag="ACHLAG_003138"
     CDS             10928..11143
                     /gene="bdm"
                     /locus_tag="ACHLAG_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFP0656318.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            11245..11382
                     /gene="sra"
                     /locus_tag="ACHLAG_003139"
     CDS             11245..11382
                     /gene="sra"
                     /locus_tag="ACHLAG_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFP0656319.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            11539..13236
                     /gene="maeA"
                     /locus_tag="ACHLAG_003140"
     CDS             11539..13236
                     /gene="maeA"
                     /locus_tag="ACHLAG_003140"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP0656320.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            13370..14380
                     /gene="adhP"
                     /locus_tag="ACHLAG_003141"
     CDS             13370..14380
                     /gene="adhP"
                     /locus_tag="ACHLAG_003141"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFP0656321.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            14527..14811
                     /locus_tag="ACHLAG_003142"
     CDS             14527..14811
                     /locus_tag="ACHLAG_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP0656322.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(14862..15515)
                     /gene="fdnI"
                     /locus_tag="ACHLAG_003143"
     CDS             complement(14862..15515)
                     /gene="fdnI"
                     /locus_tag="ACHLAG_003143"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFP0656323.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(15508..16392)
                     /gene="fdnH"
                     /locus_tag="ACHLAG_003144"
     CDS             complement(15508..16392)
                     /gene="fdnH"
                     /locus_tag="ACHLAG_003144"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFP0656324.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(16405..19452)
                     /gene="fdnG"
                     /locus_tag="ACHLAG_003145"
     CDS             complement(16405..19452)
                     /gene="fdnG"
                     /locus_tag="ACHLAG_003145"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(18865..18867),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP0656325.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            19684..20565
                     /gene="yddG"
                     /locus_tag="ACHLAG_003146"
     CDS             19684..20565
                     /gene="yddG"
                     /locus_tag="ACHLAG_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFP0656326.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         5056 a         5278 c         5272 g         5089 t
ORIGIN      
        1 tcgatctcgc acgttgcgtt tcgttttgat tccgtctgat ttcccagcct tataagggtg
       61 tgaattatgg agatgtattt taaaagaatg aaagatgagt ggaccggact tgtcgaacag
      121 gcagatcctc tcattcgtgc taaagccgca gaaattgccg ttgcgcatgc tcattatctg
      181 agtatcgagt tttatcgaat tgtccgcatc gacccgcatg ccgaagaatt cttgagtaat
      241 gaacaagttg agcggcagtt gaaaagtgcg atggaacgct ggattattaa cgtgctttct
      301 gcccaggttg acgatgtcga aaggctaata caaatccagc ataccgtcgc ggaagtgcat
      361 gcccgcatag gaattccggt agaaattgtc gagatggggt ttcgggtgct gaaaaaaatc
      421 ctctatccgg taatcttctc ttcggattat tccgccgcag aaaaacttca ggtctaccat
      481 ttctcgatta acagtattga tatcgctatg gaagtgatga cccgcgcgtt tacctttagt
      541 gacagtagtg cctcaaagga agatgaaaac tatcgtatct tctcgttact ggaaaacgcc
      601 gaagaagaaa aagaacggca aatagcctca atactttcat gggaaataga tattatctat
      661 aaagtcctgc tggattctga tttaggcagc agtttgcctt taagccaggc tgattttggc
      721 ctgtggttta accataaagg tcgacattat tttagcggca ttgccgaagt aggccatatc
      781 tcccgtctga ttcaggattt cgacggtatt ttcaatcaaa ccatgcgtaa caccaggaat
      841 ttgaataaca gaagtttgcg ggtgaagttt ctattacaga taagaaatac cgtatcgcaa
      901 attattacct tgctgcgtga attgtttgaa gaagtatcgc gccacgaagt cggtatggat
      961 gtactgacga aattacttaa ccgccgtttc ctgccgacta tcttcaaacg cgaaattgcc
     1021 catgccaacc ggaccggtac accgctgtca gtgctgatta ttgacgttga taaattcaaa
     1081 gagatcaacg atacgtgggg ccataacact ggcgatgaaa ttctgcgtaa agtctctcag
     1141 gccttttatg acaacgtccg cagtagtgat tatgttttcc gctacggggg cgatgaattt
     1201 atcattgttt tgactgaagc ttctgaaaac gaaacgttac gtaccgcaga acgtattcgc
     1261 agtcgggtgg agaaaaccaa actgaaagcc gcaaacggcg aggatattgc cctctcactt
     1321 tccatcggtg ccgccatgtt taatggtcat cctgactatg agcgcctcat tcaaatagcc
     1381 gatgaagctc tgtatatcgc taaaagacga ggtagaaacc gtgttgaact ctggaaagcc
     1441 agtctttaga tgcgccagga tgcagaggta atcatgaagc taaccgatgc ggataatgcc
     1501 gccgatggca tttttttccc cgcccttgag caaaatatga tgggtgcggt gttaattaac
     1561 gaaaataatg aagtgatgtt tttcaacccc gccgcagaga agctctgggg gtacaaacgt
     1621 gaagaagtca ttggcaataa cattgatatg ctgattccgc gggatttgcg tcctgcgcat
     1681 cctgaataca ttcgtcataa ccgtgaaggc ggtaaagcgc gtgtcgaggg gatgagtcgg
     1741 gagctgcagc tggagaaaaa agacggcagt aaaatctgga cccgttttgc gctatcgaaa
     1801 gtgagcgccg aggggaaagt ttattacctg gcgctggtac gggatgccag cgtagaaatg
     1861 gcgcaaaaag aacagactcg acaattgatt attgccgttg accatctcga ccgaccggtg
     1921 attgtcctcg atccggaacg ccatattgtg cagtgcaatc gcgcatttac cgaaatgttt
     1981 ggttactgca ttagcgaagc cagcggtatg cagcccgata cactcctgaa cattcctgaa
     2041 ttccctgccg ataaccgcat tcgtttacaa cagttgctat ggaaaaccgc ccgcgatcag
     2101 gatgaatttc tgctgttgac gcgcaccggt gaaaaaatct ggattaaagc ctctatcagc
     2161 ccggtttatg acgtgctcgc gcatctgcag aacctggtaa tgaccttctc ggatatcacc
     2221 gaagaacggc aaatccgcca gcttgaaggc aatattctcg ccgccatgtg cagcagcccg
     2281 ccatttcatg aaatggggga aatcatttgt cgtaacatcg aatctgttct caacgaatcg
     2341 catgtttcgc tgttcgcact gcgcaacggg atgccgatac actgggcgtc atcttcgcac
     2401 ggtgcagaag ttcaaaatgc gcaaagctgg tcagcgacca ttcgtcagcg tgatggcgcg
     2461 cctgcgggga tcctgcaaat taaaacctcg tcaggagcag aaaccagcgc ctttatcgaa
     2521 cgcgtggcag atatcagtca gcatatggcc gcgctggcgc tggaacagga aaaaagccgc
     2581 cagcatattg aacaactcat ccaatttgat ccgatgaccg gtctgccaaa tcgcaataac
     2641 ctgcacaatt acctcgatga cctggtcgac aaagccgtct ctccggtggt gtatctcatc
     2701 ggtgttgacc atattcagga tgtgattgat agccttggct atgcgtgggc cgatcaggca
     2761 ttgctggaag tggtcaatcg ctttcgtgaa aaactcaaac cggatcagta tctctgtcgt
     2821 atcgaaggta cgcagtttgt cctcgtgagc ctggaaaacg acgtcagtaa cattacccaa
     2881 atcgccgatg agctacggaa tgtggtcagc aagccgataa tgattgacga taaacccttc
     2941 ccgcttacct tgagtattgg catcagctac gacgtgggta aaaaccgcga ttacctgctc
     3001 tccactgctc acaatgcaat ggattatatt cgcaagaatg gcggtaacgg ctggcagttc
     3061 ttcagcccgg cgatgaacga aatggtaaaa gagcgtttgg ttttaggcgc agcgctgaaa
     3121 gaagcgatta gcaataacca actgaaactt gtttaccagc cgcaaatctt cgcagaaacg
     3181 ggtgaactgt acggcatcga agcccttgcg cgctggcacg atccccagca tggtcatgtg
     3241 cccccttcac ggtttattcc tctcgcagaa gagattggtg aaatcgaaaa tattgggcgc
     3301 tgggtcatcg cggaagcttg ccgtcagtta gcagaatggc gtagccagaa tattcatatc
     3361 ccggcgcttt ccgttaactt gtcggcactg cactttcgca gtaatcaact gcctaatcag
     3421 gtgtctgatg caatgcacgc ctggggtatt gacggccacc agctgacggt agaaatcacg
     3481 gaaagcatga tgatggaaca cgataccgaa atctttaagc gcattcagat cctgcgtgat
     3541 atgggcgtgg gcttatcggt agatgatttt ggtacgggct tttccggatt atcccgctta
     3601 gtcagtcttc cggtaacgga aatcaaaatt gacaaaagtt ttgtcgatcg ttgtctgacc
     3661 gaaaaacgca tccttgcctt acttgaagcc attaccagca ttgggcaaag cctcaattta
     3721 accgtcgtgg cggaaggcgt cgaaaccaaa gagcaatttg agatgctacg caagatccac
     3781 tgtcgcgtta ttcagggata tttcttttcc cgccccctac ccgccgaaga aattccaggc
     3841 tggatgagca gcgtgttacc gctgaaaatc tgacaaattc ctctcgcccg cactcgcggg
     3901 ttttcattta acgtgacact gtcacttaaa tactgtgatt tcagctacga ttccgggaga
     3961 ttccttcctt aacttcccct cttgcaccaa aactgcacta cgctgcacaa atgttgatca
     4021 aaagttaaaa aatataactt taagccagca ttctggcgct tatcccggca tggcatgaga
     4081 tctgcataag cggaaagcgc agcaattttt gtcttataca ggaaccgttt atgtcggata
     4141 ccaccgaact ggttgattta gccgtaatct tccctgatct ggagatcgaa ttgaaatacg
     4201 cctgcgctga taacatcaca ggtaaagcta tttatcagca agcgcgttgt ctgctacaca
     4261 aggatgcgat taccgcgctg gcgaaaagta tcagtatcgc ccagctgtca ggcttacaac
     4321 tggtgattta cgatgcgtat cgcccacaac aagcacaggc gatgttgtgg caagcctgcc
     4381 cagacccgca atatgttgtt gatgtgacgg tcggttctaa tcacagccgt ggcacggcga
     4441 tcgacctgac gcttcgtgat gagcacggga acatcctcga tatgggcgca gggttcgatg
     4501 aaatgcacga gcgctcccat gcctatcacc cttccgtccc gcccgccgct cagcgcaatc
     4561 ggctgttgct gaatgcgata atgactggtg gcggttttgt cggtatctac agcgaatggt
     4621 ggcacttcga attaccccag gcagcgagtt accctctgct cgctgatcaa ttcacctgtt
     4681 ttatatcccc tggcacacaa cacgtcagtt aatcaggaaa ctgtcatgaa gagatcgata
     4741 ccgtttcgtc ccacattgct cgcgctcgtc cttgccacaa atttcccggt tgcgcacgcc
     4801 gccgtaccga aagatatgct ggtgattggt aaggccgccg atccacaaac cctcgacccg
     4861 gcggtaacaa tagataataa cgactggacg gtgacctacc cgtcttatca gcggctggtt
     4921 cagtacaaaa cggacggtga taaaggctca accgacgttg aaggcgatct ggcaagtagc
     4981 tggaaagcgt ctgacgatca aaaagagtgg acgttcaccc tgaaggacaa tgctaaattt
     5041 gccgatggca caccggtcac tgccgaagca gtaaaactct cttttgagcg gctactaaaa
     5101 atcggccagg ggccagcaga agcatttccc aaagatttaa agattgatgc tcccgacgaa
     5161 catacggtga agtttaccct tagccagcca ttcgcaccgt tcctctacac gctggcgaat
     5221 gacggtgcat ccattatcaa tccggcggtc ttaaaggaac atgcagcgga tgatgctcgc
     5281 ggcttcctcg cgcaaaatac cgccggttcc ggaccattta tgctgaaaag ctggcaaaaa
     5341 ggtcagcaat tagttctggt gccaaatccg cattaccccg gcaataaacc gaactttaag
     5401 cgagtatcgg taaaaattat cggtgaaagt gcctcccgtc gcctgcagct ctcccgtggc
     5461 gacattgaca ttgccgatgc gctgccggtg gatcaactca acgccctgaa gcaggaaaat
     5521 aaagtcaatg tggcagagta tccgtcactg cgcgttacct atctgtatct gaataacagc
     5581 aaagcgcctc ttaatcaggc ggatctgcgt cgggccattt cctggtctac cgattatcag
     5641 ggcatggtta acggcattct gagtggtaac ggaaaacaga tgcgcggccc gattccggaa
     5701 ggcatgtggg gctacgatgc gacggcaatg caatacaacc atgacgaaac gaaagccaaa
     5761 gccgaatggg ataaagtaac gagcaaaccc accagcctga cgtttctcta ctccgataac
     5821 gatccgaact gggagcctat tgctctggcg acacaatcca gtctcaacaa gctgggcatc
     5881 aatgtgaagc tggaaaagct ggcgaacgcc accatgcgcg acagagtggg taaaggtgat
     5941 tacgacattg cgattggcaa ctggagtccg gattttgccg acccgtatat gtttatgaat
     6001 tactggtttg agtcagacaa aaaaggtctg ccgggtaacc gctcgttcta tgaaaacagt
     6061 gaggtcgata agttactgcg caatgcgctt gcgaccaccg accagacgca gcgtacccgg
     6121 gactaccagc aggcacagaa aatcgtcatt gatgacgctg cttatgtgta cctgttccag
     6181 aaaaactacc aactggcgat gaacaaagag gtgaaaggct ttgtgttcaa tcccatgctg
     6241 gaacaggtct tcaatatcaa taccatgagt aaataacgtg accttctgga gtattttacg
     6301 ccaacgctgc tgggggctgg tgctcgtggt ggcgggcgtc tgcgtgatta cgtttattat
     6361 ctcgcatctg atccctggcg atccggcgcg gttactggcg ggtgaccgcg ccagcgatgc
     6421 tatcgtggaa aatattcgcc agcaactggg actggaccag ccactgtatg tacagtttta
     6481 ccgctacgtc agcgacctgt ttcatggtga cctgggaaca tccattcgta ccgggcgtcc
     6541 ggtgctggaa gagttgcgta tatttttccc ggcgacgctg gagctggctt ttggtgccct
     6601 gctgctggca ctcctgattg gcatcccgct gggcatactc tctgcagtct ggcgaaatcg
     6661 ctggctggat catctggtgc gaataatggc cattaccgga atctccacac ctgcgttctg
     6721 gcttgggctg ggcgtcattg tgctgtttta tggtcatctg caaattcttc ccggcggcgg
     6781 aaggcttgat gactggctgg atccaccaac gcacgttacc ggcttttatc tgctcgatgc
     6841 actgcttgaa ggcaacggtg aagtcttctt caatgcgttg caacatctca tcttaccggc
     6901 attaacgctg gcgttcgttc acctgggaat tgtcgctcgc cagatccgct cagcgatgct
     6961 ggaacaattg agtgaagact acattcgtac cgcccgggcc agcggcttgc ccggctggta
     7021 tatcgtttta tgttatgcgc tacccaatgc gttgatccca tcgattaccg tattgggttt
     7081 ggcgctgggc gatttgttgt atggcgcagt gctcaccgaa accgtttttg cctggcccgg
     7141 aatgggtgca tgggtagtaa catcaataca ggcgctcgac ttcccggcag tgatgggctt
     7201 tgccgtcgtg gtttcatttg cttatgtgct ggtcaacctg gtggtggatt tgctctattt
     7261 gtggattgat ccgcgtatcg gacgtggagg tggtgaatga tgctaagcga ggaaacgtcc
     7321 gccgtacgcc cacaaaaaca aacgcgattt aacggtgcaa aactggtatg gatgctgaaa
     7381 ggcagtccgc tcaccgtgac cggcgcagtc atcattgtat taatgctatt gatgatgatt
     7441 ttttcaccgt ggctggcgac gcatgatccc aacgccattg atttaaccgc ccgccttttg
     7501 ccgccttctg cggcgcactg gtttggcacc gatgaagtgg gacgcgatct gttcagccgc
     7561 gtactggtcg gcagtcagca atcaattctc gccggattag tcgtggttgc cattgcgggt
     7621 atgattggtt cgctactcgg atgtctatcc ggtgtgcttg gcggacgcgc agacgccatc
     7681 atcatgcgca tcatggacat tatgctgtcg attccttcgt tggtactgac aatggcactg
     7741 gctgccgctc tcgggccgag tttatttaac gccatgctgg cgattgctat tgtgcgaatt
     7801 cccttttatg tgcgcctggc gcgggggcaa gcattagttg tacgccagta tacttatgtt
     7861 caggcggcga aaacctttgg cgcgtctcgt tggcatctga tcaactggca tattttacgt
     7921 aactccctgc cgccgctgat cgtgcaggca tcgctggata tcggtagcgc gattttaatg
     7981 gccgccacgt tgggatttat tggcctgggt gcacaacaac cgagtgctga atggggcgcg
     8041 atggtagcga atggtcgcaa ctatgtactc gatcagtggt ggtattgcgc atttccgggg
     8101 gcagcgattt tgcttaccgc cgtcgggttt aatctctttg gtgatggtat tcgcgatctg
     8161 cttgacccga aagcaggagg aaagcagtca tgacccaacc cgttctggac attcaacagc
     8221 tgcatttgag tttccccggt tttaacggtg atgttcacgc gctcaacaat gtgtccttgc
     8281 agattaaccg cggtgaaatt gtcggtctgg tgggagaatc cggctcaggt aaatcagtca
     8341 ccgcaatgct gattatgcgt ctgctacaga cgggcagtta ttgcgtacat cgggggcaga
     8401 tttcactgct gggagacgat gttcttaacg ctcgggaaaa acagcttcgt cagtggcgcg
     8461 gcgcacgagt ggcgatgatc tttcaggaac cgatgaccgc cctcaatccg acacttcgaa
     8521 taggtcttca gatgatggac gtgatccgcc atcatcaacc aataagtcgt cgggaagcca
     8581 gagctaaagc gattgccctg ctggaagaga tgcaaatccc ggatgccgtg gaagttatgt
     8641 cgcgctatcc gtttgagctt tcaggtggta tgcgccagcg ggtaatgatt gcgctggcat
     8701 tctcctgcga gccgcaattg attattgccg acgaaccgac tacggcgctg gacgtcacgg
     8761 tgcagttgca ggtactgcgt ttgcttaaac ataaagcccg cgccagtgga actgcggttc
     8821 tgttcatcag ccatgatatg gccgtggtgt cgcaactgtg cgatagcgtt tacgtgatgt
     8881 atgccggaag cgtaattgaa agcggcgtga cggcagatgt tatccatcat ccccggcatc
     8941 cgtataccat tggtttgctc caatgcgcac cagaacatgg agtaccacgc cagctattac
     9001 ccgccattcc agggacggta ccaaacctca cccatttgcc tgaaggctgc gcttttcgcg
     9061 atcgttgcta tgcggcaggt gcacagtgtg aaaacgtccc ggcgctgaca gcgtgtggtg
     9121 acaacaacca gcgctgcgcc tgttggtatc cgcagcagga ggtcataagt gtctgacacg
     9181 ttattaacgt tacgcgacgt ccatatcaat ttcccggccc gtaaaaactg gcttggtaaa
     9241 actacggaac atgttcatgc cattaatggt attgatttac agatccgccg tggtgaaacc
     9301 ttagggatcg tcggcgagtc aggctgcggc aaaagcaccc tcgcacagct tttaatgggc
     9361 atgctgcaac cgagccacgg gcagtacatc cgttctggct cacaacgcat tatgcagatg
     9421 gtgtttcagg acccgctctc ttcgctcaat ccgcgcttac cggtgtggcg catcatcaca
     9481 gaaccgctct ggatagctaa gcgtagtagt gaacaacagc ggcgagcgtt ggcagaggag
     9541 ctggctgtgc aggtgggtat tcgtccggag tatctcgacc gcctgcctca tgcgttctcc
     9601 ggcgggcagc ggcaacgcat cgccattgcc agagcactct cttcgcagcc tgacgtgatt
     9661 gtgcttgatg agccaacctc ggcgctggat atctccgtgc aggcgcagat cctcaattta
     9721 ctggtaacgc tacaggaaaa tcacgggctg acttatgtgc tgatttcaca caatgtctcg
     9781 gtgatacgtc atatgagcga tcgggtggcg gtgatgtatc tcgggcagat tgtggaacta
     9841 ggggaagcgc aacaggtgtt gactgctccg gcacatccat atacccgatt attgctggat
     9901 tccctccccg ccattgataa accgctggag gaagaatggg cattacgtaa aacggatctg
     9961 ccgggaaacc gcacgttgcc gcaaggctgt tttttctacg aacgttgccc tctggcaacc
    10021 cacggatgtg aagtccggca atcattaaca ataagggagg acggacgtga gatccggtgc
    10081 tggcgggcgc tgtagagatg ctaacaacgc atcaggcatt cacgcgcctg acgcgtcatc
    10141 cggcaatgct ttacgatttc aactggtaat caagcgtaat ttccgctttc agtacctgag
    10201 aaaccgggca tcctgctttt gctttctgga tgatgccgtc aaaggtagag gcatcaatac
    10261 ccggcaccgc aacttcactc ttcagtgcga ttttcgtaat cgcaaaaccg gcatccactt
    10321 tatccagcga cacatcggcg gtggtatcaa tcgatgttgg cgtaaatccc gcttccccca
    10381 gcattaatga aagcgccatt gagaaacatg cggcatgcgc tgcgccaatc agttcttcag
    10441 ggttagttcc tttttcgcct tcaaaacgcg tgttaaatcc atacggctgt tggttcagca
    10501 caccactctc ggtggatact gttcccttcc cgcgtttgat atcgccttcc cagtgtgcct
    10561 gacctttctt atggattgtc attgttgctc tcctgtgggc ttaaaaagga aattatagac
    10621 tacgtgagaa acagcacgag gataagcagg atattccgaa taaaaatccg gcagaaatat
    10681 tacttctgca cttaaattaa atttccacaa tagataaccg ggaatatata acccaagaga
    10741 tattgcatta atattaatca actcaccaca ataataatac ctatcaatat catgaggtta
    10801 aaatataaaa atcatcaacc aagactaatc ttaacaacga agcggcaaat atttgccatg
    10861 ctgaatgtgc tttataaatc tgcgatccgt agcagacacc ataaatacac agacacggag
    10921 aatcactatg tttacttatt atcaggcaga aaattcaaca gcagaacccg ctctggttaa
    10981 tgccattgag caaggccttc gggcagagca cggtgttgtc actgaagatg acattctcat
    11041 ggagctgacc aaatgggtgg aagcttctga taacgacatc ctcagtgata tctaccagca
    11101 aacgatcaat tatgtggtca gtggccagca ccctacgctt taaggtgcta tgcttgatcg
    11161 gcaacctaat ttaggggttt agcacgtgtt tcttcgctac ggcgatgttg tccttaaaac
    11221 tagcaacagg attgaggagt taaaatgaaa tcgaaccgtc aggcacgtca tattcttgga
    11281 ctggaccata aaatttctaa ccagcgcaaa atagttaccg aaggtgacaa atccagcgtg
    11341 gtaaataatc caaccggcag aaaacgcccc gctgaaaagt aattcataac catcagtcct
    11401 caatgacgat taaacaccat tgcctgcgca atggtgtttt tgtttttatc tgctctatac
    11461 ttggggccga cgccctggcg gtaaagcaaa gacgataaaa gcgtgccagg gatggatatt
    11521 caaaaaagag tgagtgacat ggaaccaaaa acaaaaaaac agcgttcgct ttatatccct
    11581 tacgctggcc ctgtactgct ggaatttccg ttgttgaata aaggcagtgc cttcagcatg
    11641 gaagaacgcc gtaacttcaa cctgctgggg ttactgccgg aagtggtcga aaccatcgaa
    11701 gaacaagcgg aacgagcatg gatccagtat cagggattca aaaccgaaat cgacaaacac
    11761 atctacctgc gtaacatcca ggacaccaac gaaaccctct tctaccgtct ggtaaacaat
    11821 catcttgatg agatgatgcc tgttatttat accccaaccg tcggcgcagc ctgtgagcgt
    11881 ttttctgaga tctaccgccg ttcacgcggc gtgtttatct cttaccagaa ccggcacaat
    11941 atggacgata ttctgcaaaa cgtgccgaac cataatatta aagtgattgt ggtgactgac
    12001 ggtgaacgta ttctggggct tggtgaccag ggcatcggcg ggatgggcat tccgatcggt
    12061 aaactgtcgc tctataccgc ctgtggcggc atcagcccgg cgtataccct tccggtggtg
    12121 ctggatgtcg gaacgaacaa ccaacagctg cttaacgatc cgctgtatat gggctggcgt
    12181 aatccacgta tcactgacga tgaatactat gaattcgttg atgaatttat ccaggctgtg
    12241 aaacaacgct ggccggacgt gctgttgcag tttgaagact ttgctcaaaa aaatgcgatg
    12301 ccgttactta accgctatcg caatgaaatt tgttctttta acgatgacat tcagggcact
    12361 gcggcagtaa cagtcggcac actgatcgca gccagccgcg cggcaggtgg tcagttaagc
    12421 gagaaaaaaa tcgtcttcct tggcgcaggt tcagcgggat gcggcattgc cgaaatgatc
    12481 atctcccaga cccagcgcga aggattaagc gaggaagcgg cgcggcagaa agtctttatg
    12541 gtcgatcgct ttggcctgct gacggacaag atgccgaacc tgctaccttt ccagaccaaa
    12601 ctggtgcaga agcgcgaaaa cctcagtgac tgggataccg acagcgatgt gttgtcactg
    12661 ctggatgtgg tgcgcaatgt aaaaccagat attctgattg gcgtctcagg acagaccggg
    12721 ctgtttacgg aagagatcat tcgtgagatg cataaacact gtccgcgtcc gatcgtgatg
    12781 ccgctgtcta acccgacgtc acgcgtggaa gccacaccgc aggacattat cgcctggacc
    12841 gaaggtaacg cgctggtcgc cactggcagc ccgtttaatc cagtggtatg gaaagataaa
    12901 atctacccta tcgcccagtg taacaacgcc tttattttcc caggcatcgg gctgggtgtt
    12961 attgcttccg gcgcgtcacg tatcaccgat gagatgctga tgtcagcaag tgaaacgctt
    13021 gctcagtatt cgccgctggt gctgaacggc gaaggtctgg tactaccgga actgaaagat
    13081 attcagaaag tctcccgcgc aattgcgttt gcggttggca aaatggcgca gcagcaaggc
    13141 gtggcggtga aaacgtctgc cgaagctctg caacaagcca ttgacgataa tttctggcaa
    13201 gccgaatacc gcgactaccg ccgtacctcc atctaagcct gcgcccggta gtgaaggcta
    13261 ccgggctatt tccctctccc tttttcagat ctcatccata ctgggtagtg gcgaataaat
    13321 ctcatttgcc tcacctgcta tgcagaacat catccgaaaa ggaggaacta tgaaggctgc
    13381 agttgttacg aaggatcatc atgttgacgt tacggataaa acactgcgct cactgaaaca
    13441 tggcgaagcc ctgctgaaaa tggagtgttg tggtgtatgt cataccgatc ttcatgttaa
    13501 gaatggcgat tttggtgaca aaactggggt aattctgggc catgaaggca tcggtgtggt
    13561 ggcagaagtg ggtccaggtg tcacctcatt aaaaccaggc gatcgtgcca gcgtggcgtg
    13621 gttctacgaa ggatgcggtc attgcgaata ctgtaacagt ggtaacgaaa cgctctgccg
    13681 ttcagttaaa aatgccggat acagcgttga tggcgggatg gcggaagagt gcatcgtggt
    13741 cgccgattac gcggtaaaag tgccagatgg tctggactcg gcggcggcca gcagcattac
    13801 ctgtgcgggg gtcaccacct acaaagccgt taaactgtca aaaattcgtc ccggtcagtg
    13861 gattgctatc tacggtcttg gcggtctggg taacctcgcc ctgcaatacg cgaagaatgt
    13921 ctttaacgcc aaagtgatcg ccattgatgt caatgatgag cagttaaaac tggcaaccga
    13981 aatgggtgca gatttagcga ttaactcacg caccgaagac gccgccaaaa ttgtgcagga
    14041 gaaaaccggt ggtgcacacg ctgcggtggt gacagcggta gctaaagctg cgtttaactc
    14101 ggcagttgat gctgtccgtg caggcggtcg tgttgtggct gtcggtctgc cgccggagtc
    14161 tatgagcctg gatatcccac gccttgtgct ggatggcatt gaagtggtcg gttcgctggt
    14221 cggcacgcgc caggatctaa ccgaagcctt ccagtttgcc gccgaaggca aagtggtgcc
    14281 gaaagtcgcc ctgcgtccgt tagcggacat caacaccatc tttaccgaga tggaagaagg
    14341 caaaatccgt ggccgtatgg tgattgattt ccgccgttaa gaggcctttg ctgcgagtgc
    14401 cagtcctggt gtcgcagcat cgcactctct ccgacattaa atggctaaat ggattattgt
    14461 tccttcctac gcaacaataa tgatagtgtt cctgatgaag gaacactact gagagtaaag
    14521 acactgatga aaatggccaa tcatccccgc ccgggggaca ttattcagga atcactggac
    14581 gaacttaatg tcagcctgcg cgagtttgcc agagcaatgg aaattgcgcc ctcaacggca
    14641 agccgattgc tgaccggaaa agcagctttg acaccagaaa tggcgattaa actttccgtg
    14701 gtgatcggca gttcgccgca aatgtggctg aatctgcaaa acgcctggag tctggcagag
    14761 gcagaaaaaa cggtggatgt gtcgcgacta cgccgtctgg ttacgcaata aaaaaatggc
    14821 gcttcttaaa gcgccatttt cagcttaaag ttcaaaagag attatatccc ttcttcactc
    14881 tcttttttcg cttctgcctt ctcgatttca cgataccagc gcggatggtg tttcttcgcc
    14941 cagcgacggc tcaccttccc ttcgatcatc cctttaatcg accctttcac ccagaatgcc
    15001 atatacatat ggatcaggat ggcgtggatc aggatgatac ccgcagccgc gtggatcagc
    15061 aggctgtagc gaacaacctg catcgggaag tactgcgcaa agtacggacg ccagataatc
    15121 accccggtca ccagcagcac gaaaatcatg ctcatgatcg accagaacat catcttttgc
    15181 ccggcgttgt acttaccgac atccgccact ttatgctcat tgcctttcaa tacttcgaca
    15241 atgttcaaca gccacggaat atctttctta tccgggatgt tgtgatgcac aaaacgcaca
    15301 aacataaaca tcagtgcgac gaaaatcgca atgccgaaaa acgggtgcaa aatgcgtccc
    15361 atctgcggcg taccgaaggt ttgcgtcagc cattgcagcg tcgggaagaa gaacgaaatc
    15421 ccggacagcg ccaccaggaa gaagcaaatc accacggtcc agtgacaggc gcgatcaata
    15481 aatttggtgc gcacaatcat tttcgactta ctcatgatga tcctcctcgt catcgtccac
    15541 ttccttattc gggccaatac cgatgtagtg gaaaatcaac ccggcaaaag tggcaataaa
    15601 gccagccgct gccagcggtt tcaacgcgcc tttccacagg cttaccgagg tgtcgatctt
    15661 cggatctttc ggcagaccgt gatacagctc cggctgatcg gcgtgatgca gcacgtacat
    15721 aacgtgcgta ccaccgaccc cttccgggtt gtagacgcca gcatgttcgt aaccacgcgc
    15781 tttcagtttc gccacgcgct gttccgccag ctccagcatc tccttcttgg tgccgaagtg
    15841 gatagccccg gtcggacagg ttttcacaca agccggttcc tggccgacgc tgacgcgatc
    15901 gacgcagagc gtgcatttat atacccggtt atcctctttg ttgaggcgcg gaatattaaa
    15961 cggacacccg gcaatgcagt aaccacagcc gatgcagttt tccgactgga aatcgacaat
    16021 cccgttagcg tactgaatga ttgcaccagc agacgggcac gccttcaggc agccgggatc
    16081 ttcacagtgc atacagccgt ctttacggat cagccactcc agcttgccgt tctgttcggt
    16141 ttcgctaaag cgcatcaccg tccaggactt ggcgctcaga tcggcggggt tatcgtaaac
    16201 cccgacgcag tgccccactt catcacggat gtcgttccac tccgaacacg ccacctgaca
    16261 ggctttacag ccgatacagg tggaaacgtc gataagtttt gcgacttctg ctttgtaatc
    16321 acgcacctga gaaggcggcg tgatggagtt agttgcggac cttttgataa tgtcctgcgt
    16381 ttccatagcc atctgttcgc ccccttacgc cttctcgatg ttgactaaga acgctttata
    16441 ttccggcgtt tgcgagtttg catcaccgac attcggcgtc agagtgttag cgatataacc
    16501 tttacgcgcg acaccctcaa agccccagtg gattggaata cccaccgttt caacctgctg
    16561 accatttaca ttcagtggtt tcagacgacg cgttaccaca gccaccgcgc ggataaagcc
    16621 acgcttgctt gagacagtga cacgatcgcc attattaatg cctttcgccg ccgccagcgt
    16681 ttcgctgatt tccacaaact gttccggctg agcaattgcg ttgagcaatg cgtgcttggt
    16741 ccaggtgtgg aagtgctcgg tcagacgata ggtcgtaccc acatacggga actgctcttt
    16801 tttacccatc cgcagtgcgt cttgttcata cagacgaaca accgggttag acaccacgtt
    16861 cggatgcagc gggttagtgc ccagcggcgt ttcaatcggc tcgtagtgtt ccgggaacgg
    16921 accttccgcc attttgttga tggcaaacag gcgtcccatc ccttccggct gcatgataaa
    16981 cggcccggtt ggcgtacccg gtgcggcatt gccgaagtca ggaatatcgt tacccgtcca
    17041 cttgctgccg ttccactgga tcagcatccg tttcggatcc cacggtttac cgttgatatc
    17101 cgccgaagca cggttgtaca gcacgcgacg gttgagcggc cacgcccagg cccatcccag
    17161 cgtattcccc agaccggacg ggtcggagtt atcgcggtta gccatctggt tgccctgctc
    17221 tgtccagcta ccggtgtaga tccagcaaga agatgcggtt gtaccgtcat cacgcagatg
    17281 cgcaaagcta ctcagcaact gacctttctt cgcaatcagc acgccattag catcatagag
    17341 atcttccagc gcatagccgt tgttctcttt agctacttcg tcagattgcg gttcgtgcgg
    17401 ctgcttgtag ttccagctca tcttcatcag cggttctacg cctttaccac cttcggcctg
    17461 gtacagctcg cgcaggtgat ggtagatacc cgccagaatt tcaccgtcgt tacgcgcttc
    17521 gcccggcgca tcctgacctt tccagtgcca ctgcagccag cgaccggagt tagcaataga
    17581 accatcttct tcagcaaagc aggtcgaagg cagacggaat acttcagtct gaatagacgc
    17641 cggatcgaca tcgttcgact cgccgtggtt ctgccagaag gttgaggttt cagtcaccag
    17701 cggatcgata accaccatgt acttcagctt gctcaggcag ctcaccactt tgtttttgtc
    17761 cgggaaggac gcaaccgggt taaagccctg gcagaaataa ccggtgactt tgccttcatc
    17821 catcatgttg aaatacttga tgacgtcgta ggtctggtcc cacttcggca gccagtcata
    17881 gccccagttg ttctctttct gcgcggcatc gccatagaaa gatttcatca ggctaacgaa
    17941 gaacttcgga tagttgctcc agtagttcac ctgaccagcc agcgtcgctt tcggcgtgtt
    18001 cgcttccaga tacgactgca aatcaacctg tttttctgac ggcagcgtca gataacctgg
    18061 caggctggta gagagcagac ctaagtcagt cagaccctga atgttggagt gaccacgcaa
    18121 tgcgttcacg ccgccaccgg ccatacccat gttaccgagc agcaactgga tcatcgccat
    18181 agtacggatg ttctgcgcac ccacggtgtg ctgcgtccag cccagcgcgt acaggaaggt
    18241 ggttgtgcga tccggtgcgc tggtggaggc cagcacttca cacactttca ggaagtcggc
    18301 ttttggcgta ccacagatgt tttcaacaac gtccggcgtg tagcgggaaa cgtgcgcttt
    18361 cagcaggttc cacacacagc gcggatgagt cagtgtttca tcgcgtttcg catagccgtt
    18421 ttcatcgaac tgatagttcc aggacgattt atcgtattgc cgtttttcag cgtcgtagcc
    18481 gctgaacaga ccgtcttcga aagcaaaatc atcacgcacc agcaggctgg cgttggtgta
    18541 atgtttaacg tattcggcgt tgattttgtt gttttcgatc aggtagcgca aaacgccaga
    18601 caggaacgta atgtccgtac cggaacgaat aggtgcgtaa atatccgcca cagaagcggt
    18661 acgcgtaaaa cggggatcga caacgatcaa ggttgcatcg ttgttgtttt tcgcttccat
    18721 cgcccagcgg aaaccgacgg gatgcgcttc agcagcgtta ccgcccatta ccattacgac
    18781 gttagcgttt ttgatatcca cccagtggtt ggtcatcgca ccgcgaccaa atgttggagc
    18841 aagacttgct accgttggtc cgtgtcagac gcgcgcctgg ttgtctaccg ccagcatccc
    18901 gagggagcgg gcaaatttct gtgtcagcat cccggtttcg ttgctggcac cggaggcaca
    18961 cagcataccg gtagaaagcc aacggtttac cgttacgccc tgctcgttct tttcaataaa
    19021 gttagcgtca cggtcagctt tcatcagctt tgcaatacgg gagaatgctt cttcccagct
    19081 aatgcgctgc catttgtcag aacctggcgc acgatattcc gggtagcgca gacggttttc
    19141 gctgttgacg taatccagca aaccggcccc ttttgggcac agcgcaccac ggcttaccgg
    19201 atgatccggg tcaccttcaa tgtgataaat cgcttctctg gcgtttttcg cgccatcacc
    19261 caggctatac atcaatagcc cgcaacctac ggaacagtat gtgcaggtgt tacggatctc
    19321 tttagcgcgt aataatttgt agtttcgcgc ctgagccagt gcttgcttcg gggcaaagcc
    19381 caatgccgct accgttgttc cagccatacc gcccgcgcag attttaaaaa attgtctgcg
    19441 actgacgtcc attgctttcc tcttttttca ggggtattac tgcgcgagga aactaacagt
    19501 aaaattctga atctttttgc gcgaaatcaa aaagaacacg tgtttgccac aaaataatcc
    19561 gcctgcgcaa attgacctac ctcaatagcg gtagaaaaac gcaccactgc ctgacaggcc
    19621 agttaaaaaa atgctataaa attcagctta atttttaacg gcaagagaga caaaacagcg
    19681 agcatgacac gacaaaaagc aacgctcata gggctaatag cgatcgtcct gtggagcacg
    19741 atggtaggat tgattcgcgg tgtcagcgag gggctcggcc cggtcggcgg cgcagctgct
    19801 atctattcat taagcgggct gctgttaatc ttcacggttg gatttccgcg tattcggcag
    19861 attccgaaag gctatttact cgccgggagt ctgttattcg tcagctatga aatctgtctg
    19921 gcgctttcct tagggtatgc ggcgacccgt catcaggcga ttgaagtggg tatggtgaac
    19981 tatctatggc ccagcctgac aattctcttt gccattctgt ttaatggtca gaaaaccaac
    20041 tggctgattg taccaggatt attattagcc ctggtcggcg tctgctgggt gttaggcggt
    20101 gataatgggt tacattatga tgaaatcatc aataatatta ccaccagccc attgagttat
    20161 ttcctggcgt ttattggtgc gtttatctgg gcagcctatt gcacagtaac gaataaatac
    20221 gcacgcggat ttaacggaat taccgttttt gtcctgctaa cgggagcaag tctgtgggtt
    20281 tactattttc tcacgccaca accagaaatg gtatttagca cgcccgtcat gattaaactc
    20341 atctctgcgg catttacctt aggatttgct tatgctgcat ggaatgtcgg tatactgcat
    20401 ggcaatgtca ccataatggc ggtaggctcg tattttacgc ccgtactttc ctcagcgctt
    20461 gcagccgtgc tgctcagcgc cccgctgtcg ttctcattct ggcaaggcgc gctaatggtc
    20521 tgcggcggtt ccctgctctg ctggctggcg acacgtcgtg gttaaaataa ttgccgggtc
    20581 tacggcccgg cagttaatca gataaatttc gacaatatta aaaaataaat ttcaaatacc
    20641 agtaattcac cttatatctc atttcgactt cgatcacata tcgataaata atatt
//
LOCUS       DAZGVQ010000090        20552 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000090 DAZGVQ010000000
VERSION     DAZGVQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.091"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            50..595
                     /gene="nohD"
                     /locus_tag="ACHLAG_003147"
     CDS             50..595
                     /gene="nohD"
                     /locus_tag="ACHLAG_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFP0656327.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            570..2495
                     /locus_tag="ACHLAG_003148"
     CDS             570..2495
                     /locus_tag="ACHLAG_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP0656328.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSVSRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            2492..2698
                     /locus_tag="ACHLAG_003149"
     CDS             2492..2698
                     /locus_tag="ACHLAG_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFP0656329.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2695..4296
                     /locus_tag="ACHLAG_003150"
     CDS             2695..4296
                     /locus_tag="ACHLAG_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0656330.1"
                     /translation="MKTSSIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIARRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEVFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            4277..5596
                     /locus_tag="ACHLAG_003151"
     CDS             4277..5596
                     /locus_tag="ACHLAG_003151"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP0656331.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSVDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAVAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            5606..5938
                     /locus_tag="ACHLAG_003152"
     CDS             5606..5938
                     /locus_tag="ACHLAG_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP0656332.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMPPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            5994..7019
                     /locus_tag="ACHLAG_003153"
     CDS             5994..7019
                     /locus_tag="ACHLAG_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP0656333.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSEEN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            7061..7459
                     /locus_tag="ACHLAG_003154"
     CDS             7061..7459
                     /locus_tag="ACHLAG_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP0656334.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHATRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            7471..7845
                     /locus_tag="ACHLAG_003155"
     CDS             7471..7845
                     /locus_tag="ACHLAG_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP0656335.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATITSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRRGKGDVWP"
     gene            7836..8414
                     /locus_tag="ACHLAG_003156"
     CDS             7836..8414
                     /locus_tag="ACHLAG_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0656336.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMTINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            8411..8806
                     /locus_tag="ACHLAG_003157"
     CDS             8411..8806
                     /locus_tag="ACHLAG_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP0656337.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFYEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            8814..9554
                     /locus_tag="ACHLAG_003158"
     CDS             8814..9554
                     /locus_tag="ACHLAG_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0656338.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            9570..9992
                     /locus_tag="ACHLAG_003159"
     CDS             9570..9992
                     /locus_tag="ACHLAG_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP0656339.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPKKTKLPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            9974..10408
                     /locus_tag="ACHLAG_003160"
     CDS             9974..10408
                     /locus_tag="ACHLAG_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP0656340.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            10401..12950
                     /locus_tag="ACHLAG_003161"
     CDS             10401..12950
                     /locus_tag="ACHLAG_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0656341.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQS
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAENQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAVLGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIEAKNRGLTDRQAARDATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDL
                     LRGSWMAGLKSGWSEWKESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGGASASGGTAIQAAAAKFHFATGGFTGTGGK
                     YEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQASGTFE
                     QNNHVVINNDGTNGQIGPQALKAVYDVARKAAMDVVTGQMRDGGLFSGGGR"
     gene            12947..13276
                     /locus_tag="ACHLAG_003162"
     CDS             12947..13276
                     /locus_tag="ACHLAG_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0656342.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNADLKTY
                     SVTLSVPRWEATALESFLAEHGGWKSFLWTPPYGYRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            13276..13974
                     /locus_tag="ACHLAG_003163"
     CDS             13276..13974
                     /locus_tag="ACHLAG_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0656343.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYNGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            13980..14723
                     /locus_tag="ACHLAG_003164"
     CDS             13980..14723
                     /locus_tag="ACHLAG_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0656344.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGICNDLAAASTFV"
     gene            14720..15292
                     /locus_tag="ACHLAG_003165"
     CDS             14720..15292
                     /locus_tag="ACHLAG_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0656345.1"
                     /translation="MKTGAEAIRALATQLPMFRQKLSDGWYQVRIAGRDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            15353..18751
                     /locus_tag="ACHLAG_003166"
     CDS             15353..18751
                     /locus_tag="ACHLAG_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0656346.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAS
                     DKWNAMWAVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMTDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            18818..19417
                     /locus_tag="ACHLAG_003167"
     CDS             18818..19417
                     /locus_tag="ACHLAG_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP0656347.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYIRVTDNKGKKHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            19482..>20552
                     /locus_tag="ACHLAG_003168"
     CDS             19482..>20552
                     /locus_tag="ACHLAG_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP0656348.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSNTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDASTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASAKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDASASKEAAKSSETNASSSASSAASSATAAGNSAKAAKT
                     SGTNAKSSETAAAQSASAAADSKTAAASSASAASTNAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASA"
BASE COUNT         4724 a         5241 c         6425 g         4162 t
ORIGIN      
        1 ttttggcctc tgtcgtttcc tttctctgtt tttgtccgtg gaatgaacaa tggaagtcaa
       61 caaaaagcag ctggctgaca ttttcggtgc gagtatccgt accattcaga actggcagga
      121 acagggaatg cccgttctgc gaggcggtgg caagggtaat gaggtgcttt atgactctgc
      181 cgccgtcata aaatggtatg ccgaaaggga tgctgaaatt gagaacgaaa agctgcgccg
      241 ggaagttgaa gaactgcggc aggccagcga gacagatctc cagccaggga ctattgagta
      301 cgaacgccat cgacttacgc gtgcgcaggc cgacgcacag gagctgaaaa atgccagaga
      361 ctccgctgaa gtggtggaaa ccgcattctg tactttcgtg ctgtcgcgga tcgcaggtga
      421 aattgccagt attctcgacg ggatccccct gtcggtgcag cggcgttttc cggaactgga
      481 aaaccgacat gttgatttcc tgaaacggga tatcatcaaa gccatgaaca aagcagccgc
      541 gctggatgaa ctgataccgg ggttgctgag tgaatatatc gaacagtcag gttaacaggc
      601 tgcggcattt tgtccgcgcc gggcttcgct cactgttcag gccggagcca cagaccgccg
      661 ttgaatgggc ggatgccaat tactatctcc caaaagaatc cgcataccag gaagggcgct
      721 gggaaacact gccctttcag cgggccatca tgaatgcgat gggcagcgac tacatccgtg
      781 aggtgaatgt ggtgaagtct gcccgtgtcg gttattccaa aatgctgctg ggtgtttatg
      841 cctactttat agagcataag cagcgcaaca cccttatctg gttgccgacg gatggtgatg
      901 ccgagaactt tatgaaaacc cacgttgagc cgaccatccg cgatattccg tcgctgctgg
      961 cgctggctcc gtggtatggc aaaaagcacc gggataacac gctcactatg aagcgttttt
     1021 ccaatggtcg tggcttctgg tgcctgggcg gtaaagcggc aaaaaactac cgtgaaaagt
     1081 cggtggatgt ggcgggttat gatgaacttg ctgcctttga tgaggatatt gaacaggaag
     1141 gctctccgac gttccttggc gacaaacgta ttgaaggctc ggtctggcca aagtccatcc
     1201 gtggctccac gcccaaagtg agaggcacct gccagattga gcgtgcagcc agtgaatccc
     1261 cgcattttat gcgttttcat gttgcctgcc cgcactgcgg ggaggagcag tatcttaaat
     1321 ttggcgacaa agagacgccg tttggcctca aatggacgcc ggatgacccc tccagcgtgt
     1381 tttatctctg cgagcataat gcctgcgtca tccgccagca ggagctggac tttactgatg
     1441 cccgttatat ctgcgaaaag accgggatct ggacccgtga tggcattctc tggttttcgt
     1501 catccggtga agagattgag ccgccggaca gtgtgacctt tcacatctgg acggcgtaca
     1561 gcccgttcac cacctgggtg cagattgtca aagactggat gaagacgaaa ggggatacgg
     1621 gaaaacgtaa aaccttcgtg aacaccacgc tcggtgagac atgggaagcg aaaattggcg
     1681 aacgtccgga tgctgaggtg atggcggagc ggaaagagca ttattcagcg cccgttcctg
     1741 accgtgtggc ttacctgacc gccggtatcg actcccagct ggaccgctac gaaatgcgcg
     1801 tatggggatg ggggccgggt gaggaaagct ggctgattga ccggcagatt attatgggcc
     1861 gccacgacga tgaacagacg ctgctgcgtg tggatgaggc catcaataaa acctataccc
     1921 gccggaatgg tgcagaaatg tcggtatccc gtatctgctg ggatactggc gggattgacc
     1981 cgaccattgt gtatgaacgc tcgaaaaagc atgggctgtt ccgggtgatc cccattaaag
     2041 gggcatccgt ctacggaaag ccagtggcca gcatgccacg taagcgaaac aaaaacgggg
     2101 tttaccttac cgaaatcggt acggataccg cgaaagagca gatttataac cgcttcacac
     2161 tgacgccgga aggggatgaa ccgcttcccg gtgccgttca cttcccgaat aacccggata
     2221 tttttgatct gaccgaagcg cagcagctga ctgctgaaga gcaggtcgaa aaatgggtgg
     2281 atggcaggaa aaaaatactg tgggacagca aaaagcgacg caatgaggcg ctcgactgct
     2341 tcgtttatgc gctggcggcg ctgcgcatca gtatttcccg ctggcagctg gatctcagtg
     2401 cactgctggc gagcctgcag gaagaggatg gtgcagcaac caacaagaaa acactggcag
     2461 attacgcccg tgccttatcc ggagaggatg aatgacgcga caggaagaac ttgccgctgc
     2521 ccgtgcggca ctgcatgacc tgatgacagg aaaacgggtg gcaacggtac agaaagacgg
     2581 acggcgagtg gagtttacgg ccacttccgt gtctgacctg aaaaaataca ttgcggagct
     2641 ggaagtgcag accggcatga cacagcgacg caggggacct gcaggatttt atgtatgaaa
     2701 acgtcctcca ttcccaccct tctggggccg gacggcatga catcgctgcg tgaatatgcc
     2761 ggttatcacg gcggtggcag cggatttggt gggcagttgc gggcgtggaa cccaccgagt
     2821 gaaagtgtgg atgcagccct gctgcccaac tttacccgtg gcaatgcccg cgcagacgat
     2881 ctggtacgca ataacggcta tgccgccaac gccatccagt tgcatcagga tcatatcgtc
     2941 gggtcttttt tccggctcag tcatcgccca agctggcgct atctgggcat cggggaggaa
     3001 gaagcccgtg ccttttcccg cgaggttgaa gcggcatgga aagagtttgc cgaggatgac
     3061 tgctgctgca ttgacgttga gcgaaaacgc acgtttacca tgatgattcg ggaaggtgtg
     3121 gccatgcacg cctttaacgg tgaactgttc gttcaggcca cctgggatac cagtccgtcg
     3181 cggcttttcc ggacacagtt ccggatggtc agcccgaagc gcatcagcaa cccgaacaat
     3241 accggcgaca gccggaactg ccgtgccggt gtgcagatta atgacagcgg tgcggcgctg
     3301 ggatattacg tcagcgagga cgggtatcct ggctggatgc cgcagaaatg gacatggata
     3361 ccccgtgaat tacccggcgg gcgcgcctcg ttcattcacg tttttgaacc cgtggaggac
     3421 gggcagaccc gcggtgcaaa tgtgttttac agcgtaatgg agcagatgaa gatgctcgac
     3481 acgctgcaga acacgcagct gcagagcgcc attgtgaagg cgatgtatgc cgccaccatt
     3541 gaaagtgagc tggatacgca gtcagcgatg gattttattc tgggcgcgaa cagtcaggag
     3601 cagcgggaaa ggctgacggg ctggattggt gaaattgccg cgtattacgc cgcagcaccg
     3661 gtccgtctgg gaggcgcaaa agtgccgcac ctgatgccgg gggactcact gaacctgcag
     3721 acggctcagg acacggataa cggctactcc gtgtttgagc agtcactgtt gcggtatatc
     3781 gctgccgggc tgggtgtctc gtatgagcag ctttcccgga attacgccca gatgagctac
     3841 tccacggcac gggccagtgc gaacgagtcg tgggcgtact ttatggggcg gcgaaaattc
     3901 gtcgcatccc gtcaggcgag ccagatgttt ctgtgctggc tggaagaggc catcgctcgc
     3961 cgcgtggtga cgttaccttc aaaagcgcgc ttcagttttc aggaagcccg aagtgcctgg
     4021 gggaactgcg actggatagg ctccggtcgt atggccatcg atggtctgaa agaagtacag
     4081 gaagcggtga tgctgataga agccggactg agtacctacg agaaagagtg cgcaaaacgc
     4141 ggtgacgact atcaggaagt ttttgcccag caggtccgtg aaacgatgga gcgccgtgca
     4201 gccggtctta aaccgcccgc ctgggcggct gcggcatttg aatccgggct gcgacaatca
     4261 acagaggagg agaagagtga cagcagagct gcgtaatctc ccgcatattg ccagtatggc
     4321 ctttaatgag ccgctgatgc ttgaacccgc ctatgcgcgg gttttctttt gtgcgcttgc
     4381 aggccagctt gggatcagcc gcctgacgga tgcggtatcc ggcgacagcc tgactgccgg
     4441 agaggcaccc gcggcgctgg cgttatccgt tgatgatgac ggaccacgac aggcccgcag
     4501 ttatcaggtc atgaacggca tcgccgtgct gccggtgtcc ggcacgctgg tcagtcggac
     4561 gcgggcgctg cagccgtatt cggggatgac cggttacaac ggcattatcg cccgtctgca
     4621 acaggctgcc agcgacccga tggtggacgg cattctgctc gatatggata cgccaggcgg
     4681 gatggtggca ggggcatttg actgcgctga catcatcgcc cgtgtgcgtg acataaagcc
     4741 ggtatgggcg ctggccaacg acatgaactg cagtgcaggt cagttgcttg ccagcgccgc
     4801 ctcccgacgt ctggtcacgc agaccgcccg gacaggctcc atcggcgtca tgatggctca
     4861 cagtaattac ggtgctgcgc tggagaaaca gggcgtggaa atcacgctga tttacagcgg
     4921 cagccataag gtggatggca acccctacag ccatctaccg gatgatgtcc gggaaacact
     4981 gcagtcccgg atggatgcaa cccgccggat gtttgcacag aaggtgtcgg catataccgg
     5041 cctgtccgtg caggctgtgc tggataccga ggctgcagtg tacagcggtc aggaggccat
     5101 tgatgccgga ctggctgatg aacttgttaa cagtaccgat gcgatcaccg tcatgcgtga
     5161 tgcactggat gcacgtaaat cccgtctctc aggagggcga atgaccaaag agactcaatc
     5221 aacaactgtt tcagccactg cttcgcaggc tgacgttact ggcgtggtgc aagcgacgga
     5281 gggcgagaac gccagcgctg cgcagccgga cgtgaacgcg cagatcaccg cagcggttgc
     5341 ggcagaaaac agtcgcatta tggggatcct caactgtgag gaggctcacg gacgcgaaga
     5401 acaggcacgc gtgctggccg aaacccccgg tatgaccgtg gaaacggccc gccgcattct
     5461 ggcagttgca ccacagagtg cacaggcgcg cagtgacact gcgctggatc gtctgatgca
     5521 gggggcaccg gcaccgctgg ctgcaggtaa cccggcatct gatgccgtta acgatttgct
     5581 gaacacacca gtgtaaggga tgtttatgac gagcaaagaa acctttaccc attaccagcc
     5641 gctgggcaac agtgacccgg ctcataccgc aaccgcgccc ggcggattga gtgcgaaagc
     5701 gcctgcaatg cccccgctga tgctggacac ctccagccgt aagctggttg cgtgggatgg
     5761 caccaccgac ggtgctgccg ttggcattct tgcggttgct gctgaccaga ccagcaccac
     5821 gctgacgttc tacaagtccg gcacgttccg ttatgaggat gtgctctggc cggaggctgc
     5881 cagcgacgag acgaaaaaac ggaccgcgtt tgccggaacg gcaatcagca tcgtttaact
     5941 ttacccttca tcactaaagg ccgcctgtgc ggcttttttt acgggatttt tttatgtcga
     6001 tgtacacaac agcccagctg ctggcggcaa atgagcagaa atttaagttt gatccgctgt
     6061 ttctgcgtct ctttttccgt gagagctatc ccttcaccac ggagaaagtc tatctctcac
     6121 aaattccggg actggtaaac atggcgctgt acgtttcgcc gattgtttcc ggtgaggtta
     6181 tccgttcccg tggcggctcc acctctgaat ttacgccggg atatgtcaag ccgaagcatg
     6241 aagtgaatcc gcagatgacc ctgcgtcgcc tgccggatga agatccgcag aatctggcgg
     6301 acccggctta ccgccgccgt cgcatcatca tgcagaacat gcgtgacgaa gagctggcca
     6361 ttgctcaggt cgaagagatg caggcagttt ctgccgtgct taagggcaaa tacaccatga
     6421 ccggtgaagc cttcgatccg gttgaggtgg atatgggccg cagtgaggag aataacatca
     6481 cgcagtccgg cggcacggag tggagcaagc gtgacaagtc cacgtatgac ccgaccgacg
     6541 atatcgaagc ctacgcgctg aacgccagcg gtgtggtgaa tatcatcgtg ttcgatccga
     6601 aaggctgggc gctgttccgt tccttcaaag ccgtcaagga gaagctggat acccgtcgtg
     6661 gctctaattc cgagctggag acagcggtga aagacctagg taaagcggtg tcctataagg
     6721 ggatgtatgg cgatgtggcc atcgtcgtgt attccggaca gtacgtggaa aacggcgtca
     6781 aaaagaactt cctgccggac aacacgatgg tgctggggaa cactcaggca cgcggtctgc
     6841 gcacctatgg ctgcattcag gatgcggacg cacagcgcga aggcattaac gcctctgccc
     6901 gttacccgaa aaactgggtg accaccggcg atccggcgcg tgagttcacc atgattcagt
     6961 cagcaccgct gatgctgctg gctgaccctg atgagttcgt gtccgtacaa ctggcgtaat
     7021 catggccctt cggggccatt gtttctctgt ggaggagtcc atgacgaaag atgaactgat
     7081 tgcccgtctc cgctcgcttg gtgaacaact gaaccgtgat gtcagcctga cggggacgaa
     7141 agaagaactg gcgctccgtg tggcagagct ggaagaggag cttgatgaca cggatgaaac
     7201 tgccggtcag gacacccctc tcagccggga aaatgtgctg accggacatg aaaatgaggt
     7261 gggatcagcg cagccggata ccgtgattct ggatacgtct gaactggtca cggtcgtggc
     7321 actggtgaag ctgcatactg atgcacttca cgccacgcgg gatgaacctg tggcatttgt
     7381 gctgccggga acggcgtttc gtgtctctgc cggtgtggca gccgaaatga cagagcgcgg
     7441 cctggccaga atgcaataac gggaggcgct gtggctgatt tcgataacct gttcgatgct
     7501 gccattgccc gcgccgatga aacgatacgc gggtacatgg gaacgtcagc caccattaca
     7561 tccggtgagc agtcaggtgc ggtgatacgt ggtgtttttg atgaccctga aaatatcagc
     7621 tatgccggac agggcgtgcg cgttgaaggc tccagcccgt ccctgtttgt ccggactgat
     7681 gaggtgcggc agctgcggcg tggagacacg ctgaccatcg gtgaggaaaa tttctgggta
     7741 gatcgggttt cgccggatga tggcggaagt tgtcatctct ggcttggacg gggcgtaccg
     7801 cctgccgtta accgtcgccg cggaaagggg gatgtatggc cataaaaggt cttgagcagg
     7861 ccgttgaaaa cctcagccgt atcagcaaaa cggcggtgcc tggtgccgcc gcaatgacca
     7921 ttaaccgcgt tgcttcatcc gcgatatcgc agtctgcgtc acaggttgcc cgtgagacaa
     7981 aggtacgccg aaaactggta aaggaaaggg ccaggctgaa aagggccacg gtcaaaaatc
     8041 cgcaggccag aatcaaagtt aaccgggggg atttgcccgt aatcaagctg ggtaatgcgc
     8101 gggttgtcct gtcccgccgc aggcgtcgta aaaaggggca gcgttcatcc ctgaaaggtg
     8161 gcggcagcgt gcttgtggtg ggtaaccgtc gtattcccgg cgcgtttatt cagcaactga
     8221 aaaatggccg gtggcatgtt atgcagcgtg tggccgggaa aaaccgttac cccattgatg
     8281 tggtgaaaat cccgatggcg gtgccgctta ccacggcgtt taaacagaat attgaacgga
     8341 tacggcgtga acgtcttccg aaagagctgg gctatgcgct gcagcatcaa ctgagaatgg
     8401 taataaagcg atgaaacata ctgaactccg tgcagccgta ctggatgcac tggagaagca
     8461 tgacaccggg gcgacgcttt ttgatggtcg ccccgctgtt ttttatgagg aagattttcc
     8521 ggcaattgcc gtttatctca ccggcgctga atacacgggc gaagagctgg acagcgatac
     8581 ctggcaggcg gagctgcata ttgaagtttt cctgcctgct caggtgccgg attcagagct
     8641 ggattcgtgg atggagtccc ggatttatcc ggtgatgagc gatatcccgg cactgtcaga
     8701 tttgatcacc agtatggtgg ccagtggcta tgactaccgg cgcgacgatg atgcgggcct
     8761 gtggagttca gccgatctga cttatgtcat tacctatgaa atgtgaggac gctatgcctg
     8821 taccaaatcc tacaatgccg gtgaaaggtg ccgggaccac cctgtgggtt tataagggga
     8881 gcggtgaccc ttatgcgaac ccgctttcag acgttgactg gtcgcgtctg gcaaaagtta
     8941 aagacctgac gcccggcgaa ctgaccgctg agtcctatga cgacagctat ctcgatgatg
     9001 aagatgcaga ctggactgcg accgggcagg ggcagaaatc tgccggagat accagcttca
     9061 cgctggcgtg gatgcccgga gagcaggggc agcaggcgct gctggcgtgg tttaatgaag
     9121 gtgatacccg tgcctataaa atccgcttcc cgaacggcac ggtcgatgtg ttccgtggct
     9181 gggtcagcag tatcggtaag gcggtgacgg cgaaggaagt gatcacccgc acggtgaagg
     9241 tcaccaatgt gggacgtccg tcgatggcag aagatcgcag cacggtgacg gcgacaaccg
     9301 gcatgacggt gacacccgcc agtgcttccg tagtgaaagg gcagagcacc acgctgaccg
     9361 tggcattcca gccggagggc gcaaccgaca agagcttccg tgcggtgtct gcggataaaa
     9421 caaaagccac cgtgtcggtc agtggtatga ccatcaccgt gaaaggtgtt gctgcaggca
     9481 aggtcaacat tccggtcgta tctggtaatg gtgagtttgc tgcggttgca gaaatcaacg
     9541 tcaccgccag ttaatccgga gagtcagcga tgttcctgaa aaccgaatca tttgaacata
     9601 acggtgtgac cgtcacgctt tctgaactgt cagccctgca gcgtattgag catctcgccc
     9661 tgatgaaacg gcaggcagaa caggcggagt cagacagcaa ccggaagttt actgtggaag
     9721 acgccatcag aaccggcgcg tttctggtgg cgatgtccct gtggcataac catccgaaga
     9781 agacaaagct gccttccatg aatgaagccg ttaaacagat tgagcaggaa gtgcttacca
     9841 cctggcccac agaggcaatt tctcatgctg aaaacgtggt gtaccggctg tccggtatgt
     9901 atgagtttgt ggtgaatgat gctcctgaac aggcagagga cgccgggcct gcagagcctg
     9961 tttctgcggg aaagtgttcg acggtgagct gagttttgcc ctgaaactgg cgcgtgagat
    10021 ggggcgaccc gactggcgcg ccatgcttgc cgggatgtca tccacggagt atgccgactg
    10081 gcaccgcttt tacagtaccc attattttca tgatgttctg ctggatatgc acttttccgg
    10141 gctgacgtac actgtgctca gcctgttttt cagcgatcca gagatgcatc cgctggattt
    10201 cagtctgctg aaccggcgcg aggctgacga agagcctgaa gatgatgtgc tgatgcagaa
    10261 agcggcaggg cttgccggag gcgttcgttt tggcccggac gggaatgaag ttatccccgc
    10321 ttccccggat gtggcggaca tgacggagga tgacgtaatg ctgatgacag tatcagaagg
    10381 gatcgcagga ggagtccggt atggctgaac cggtaggcga tctggtcgtt gatttaagtc
    10441 tggatgcggc cagatttgac gagcagatgg ccagagtcag gcgtcatttt tccggtacgg
    10501 aaagtgatgc gaaaaaaaca gcggcagtcg ttgaacagtc gatgagccgg caggcgctgg
    10561 ctgcacagaa agcggggatt tccgtcgggc agtataaagc cgccatgcgt atgctgcctg
    10621 cgcagttcac cgacgtggcc acgcagcttg caggcgggca gagtccgtgg ctgatcctgc
    10681 tgcaacaggg tggtcaggtg aaggactcct tcggcgggat gatccccatg ttcagggggc
    10741 ttgccggtgc gatcaccctg ccgatggtcg gggccacctc gctggcggtg gcgaccggtg
    10801 cgctggcgta tgcctggtat cagggtaact caaccctgtc cgatttcaac aaaacgctgg
    10861 tcctttctgg taatcagtca ggtctgacgg cagatcgcat gctggtcctg tccagagccg
    10921 ggcaggcggc agggctgacg tttaaccaga ccagcgagtc actcagcgca ctggttaagg
    10981 cgggggtaag cggtgaggct cagattgcgt ccatcagcca gagtgtggcg cgtttctcct
    11041 ctgcatccgg cgtggaggtg gacaaggtcg ctgaagcctt cgggaagctg accactgacc
    11101 cgacgtcggg gctgacggcg atggcgcgcc agttccataa cgtgacggcg gagcagattg
    11161 cgtatgttgc tcagttgcag cgttccggcg atgaagccgg ggcattgcag gcggcgaacg
    11221 aggccgcaac gaaagggttt gatgaccaga cccgacgcct gaaagagaac atgggcacgc
    11281 tggaaacttg ggcagacagg acagcacggg cattcaaatc catgtgggat gcggtgctgg
    11341 atattggtcg tcctgatacc gctcaggaga tgctgattaa ggcagaggct gcgtttaaga
    11401 aagcggacga catctggaat ctgcgcaagg atgattattt tgttaacgat gaagcgcggg
    11461 cgcgttactg ggatgatcgt gaaaaggccc gtcttgcgct tgaagccgcc cgaaagaagg
    11521 ctgaacagca gagtcaacag gacaaaaatg cgcagcagca gagcgatacc gaagcatcac
    11581 ggctgaaata taccgaagag gcgcagaagg cttacgaacg gctgcagaca ccgctggaga
    11641 aatataccgc ccgtcaggaa gaactgaaca aggcactgaa agacgggaaa atcctgcagg
    11701 cagattacaa cacgctgatg gcggcggcga aaaaggatta tgaagcgacg ctgaaaaagc
    11761 cgaaacagtc cggcgtgaag gtgtctgcgg gcgatcgtca ggaagacagt gctcatgctg
    11821 ccctgctgac gcttcaggca gaactccgga cgctggagaa gcatgccgga gcgaatgaga
    11881 aaatcagcca gcagcgccgg gatttgtgga aggcggaaaa tcagttcgcg gtactggagg
    11941 aggcggcaca acgtcgccag ctatccgcac aggagaaatc cctgctggcg cataaagatg
    12001 agacgctgga gtacaaacgc cagctggctg tacttggcga taaggtcacg tatcaggagc
    12061 acctgaacgc gctggcgcag caggcggata agttcgcaca gcaacaacgg gcaaaacggg
    12121 cagccattga ggcgaaaaac cgggggctta ctgaccggca ggcagcgcgg gacgccacgg
    12181 aacagcgtct gaaggagcag tatggcgata atccgctggc gctaaataac gtcatgtcag
    12241 agcagaaaaa gacctgggca gctgaagacc tgcttcgcgg gagctggatg gctggcctca
    12301 agtccggctg gagcgagtgg aaagagagtg ccacggacag tatgtcgcag gttaaaagtg
    12361 ctgccacgca gacctttgat ggtattgcgc agaatatggc ggcgatgctg accggcagtg
    12421 agcagaactg gcgcagcttc acccgttccg tgctgtccat gatgacagaa attctgctta
    12481 agcaggcaat ggtggggatt gtcggaagta taggcagcgc cattggcggc ggcgcatcag
    12541 cgtcaggcgg tacagccatt caggcagctg cggcgaaatt ccattttgcg accggagggt
    12601 ttacgggaac cggcggcaaa tatgagccag cggggattgt tcaccgtggt gagtttgtct
    12661 tcacgaagga ggcaaccagc cggattggcg tgggaaatct ttaccggctg atgcgcggct
    12721 atgccaccgg cggttatgtc ggtacaccgg gcagcatggc ggacagccgg tcgcaggcgt
    12781 ccgggacgtt tgagcagaat aaccatgtgg tgattaacaa cgacggcacg aacggtcaga
    12841 tagggccaca ggcactgaag gctgtttatg acgtagcccg taaggcggca atggatgttg
    12901 tgaccgggca gatgcgcgat ggtggtctgt tctccggagg tggacgatga aaaccttccg
    12961 ctggaaagtg aaacccggta tggatgtggc ttcggcccct tccgtaagaa aggtgcgctt
    13021 tggtgatggc tattcccagc gagcgcctgc cgggctgaat gccgacctga aaacgtacag
    13081 cgtgacgctt tctgttcccc gttgggaggc cacggcgctt gagtcgtttc tggctgagca
    13141 cgggggctgg aaatcctttc tgtggacgcc gccttatggc taccggcaga taaaggtgac
    13201 ctgcgcaaaa tggtcgtcgc gggtcagtat gttgcgtgtt gagttcagcg cagagtttga
    13261 acaggtggtg aactgatgca ggatatccga caggaaacac tgaatgaatg cacccgtgcg
    13321 gagcagtcgg ccagcgtggt gctctgggaa atcgatctga cagaggttgg tggagaacgt
    13381 tattttttct gtaatgagca gaacgaaaaa ggtgagccgg tcacctggca ggggcgacag
    13441 tatcaggcgt atcccattca ggggagtggt ttcgaactga atggcaaagg caccagtacg
    13501 cgccccacgc tgacggtttc taacctgtat ggcatggtca ccgggatggc ggaagatctg
    13561 cagagtctgg tcggcggaac ggtggtcagg cgtaaggttt atgcccgttt tctggatgcg
    13621 gtgaacttcg tcaacggaaa cagcgacgcc gatccggagc aggaggtgat tagccgctgg
    13681 cgcatcgagc agtgcagcga attgagcgcg gtcagtgcct cctttgtact gtccacgccg
    13741 actgaaacgg atggtgccgt ttttccgggg cgcatcatgc tggccaacac ctgcacctgg
    13801 acctatcgcg gtgatgagtg cggttataac ggtccggcgg tcgcggatga atatgaccag
    13861 ccgacgtccg atatcacgaa ggataaatgc agcaaatgcc tgagtggctg taagtttcgc
    13921 aataatgtcg gcaactttgg cggcttcctt tccattaaca aactttcgca gtaaatccca
    13981 tgacacagac agaatcagcg attctggcgc acgcccggcg atgtgcgcca gcggagtcgt
    14041 gcggcttcgt ggtaagcacg ccggaggggg aaagatattt cccctgcgtg aatatctccg
    14101 gtgagccgga ggcgtatttc cgtatgtcgc cggaagactg gctgcaggca gaaatgcagg
    14161 gtgagattgt ggcgctggtc cacagccacc ccggtggtct gccctggctg agtgaggccg
    14221 accggcggct gcaggtgcag agtgatttgc cgtggtggct ggtctgccgg gggacgattc
    14281 ataagttccg ctgtgtgccg catctcaccg ggcggcgctt tgagcacggg gtgacggact
    14341 gttacacgct gttccgggat gcttatcatc tggcggggat tgagatgccg gattttcatc
    14401 gcgaggatga ctggtggcgt cacggtcaga atctctatct ggataatctg gaggccacag
    14461 ggctgtatca ggtgccgttg tcatcagcac aaccgggcga tgtgctgctg tgctgttttg
    14521 gttcatcggt gccgaatcat gccgccattt actgtggtga tggcgagctg ctgcaccata
    14581 ttcctgaaca actgagcaaa cgagagaggt ataccgacaa atggcagcga cgcacacact
    14641 ccctctggcg tcaccgggca tggcgcgcat ctgcctttac ggggatttgc aacgatttgg
    14701 ccgccgcatc gaccttcgtg tgaaaacggg ggctgaagcc atccgcgcac tggccacaca
    14761 gctcccgatg tttcgtcaga aactgagcga cggctggtat caggtacgga ttgccgggcg
    14821 ggacgtcagc acgtccgggt taacggcgca gttacatgag actctgcctg atggcgctgt
    14881 gattcatatt gttcccagag tcgccggggc caagtcaggt ggcgtattcc agattgtcct
    14941 gggggctgcc gccattgccg gatcattctt taccgccgga gccacccttg cagcatgggg
    15001 ggcagccatt ggggccggtg gtatgaccgg catcctgttt tctctcggtg ccagtatggt
    15061 gctcggtggt gtggcgcaga tgctggcacc gaaagccaga actccccgta cacagacaac
    15121 ggataacggc aaacagaaca cctatttctc ctccctggat aacatggttg cccagggtaa
    15181 tgttctgccg gttctgtacg gtgaaatgcg cgtggggtca cgcgtggttt ctcaggagat
    15241 cagcacggca gacgaagggg atggtggtca ggttgtggtg attggtcgct gatgcaaaat
    15301 gttttatgtg aaaccgcctc cgggcggttt tatcgtttat ggagcatgac gaatgggtaa
    15361 aggcagcagt aaggggcata ccccgcgcga agcgaaggac aacctgaagt ccacgcagct
    15421 gctgagtgtg atcgatgcca tcagcgaagg gccggttgaa ggtccggtgg atggattaaa
    15481 aagcgtgctg ctgaacagta cgccggtgct ggacagtgag gggaatacca atatatccgg
    15541 cgtcacggtg gtgttccggg ccggtgagca ggagcagaca ccgccggagg gatttgaatc
    15601 ctccggctcc gagacggtgc tcggtacaga agtgaaatat gacacgccga tcacccggac
    15661 catcacgtcg gcaaacattg accgtctgcg ttttactttc ggcgtgcagg cactggtgga
    15721 aaccacctca aagggggaca ggaatccatc ggaagtccgc ctgctggttc agatacaacg
    15781 taacggtggc tgggtgacgg aaaaagacat caccattaag ggtaaaacca cttcacagta
    15841 tctggcctcg gtggtggtgg ataacctgcc gccgcgcccg ttcagtatcc ggatgcgtag
    15901 gatgacgccg gacagcacca cagaccagct gcagaacaaa acgctctggt cgtcatacac
    15961 cgaaattatc gatgtgaaac agtgctaccc gaacacggca ctggtcggcg tgcaggtgga
    16021 ctcggagcag ttcggcagcc agcaggtgag ccgtaattat catctgcgcg ggcgtattct
    16081 gcaggtgccg tcgaattata acccgcagac gcggcaatac agcggtatct gggacggaac
    16141 gtttaaaccg gcatacagca acaacatggc ctggtgtctg tgggatatgc tgacccatcc
    16201 gcgctacggc atggggaaac gtcttggtgc ggcggatgtg gataaatggg cgttgtatgt
    16261 tatcggccag tactgcgacc agtcagtgcc ggacggcttt ggcggcacgg agccgcgcat
    16321 cacctgtaat gcgtacctga ccacacagcg caaggcgtgg gatgtgctca gtgatttctg
    16381 ctcggcgatg cgctgtatgc cggtatggaa cgggcagacg ctgacgttcg tgcaggaccg
    16441 gccatcagat aaggtgtgga cctataaccg cagtaatgtg gtgatgccgg atgatggcgc
    16501 gccgttccgc tacagcttca gcgccctgaa ggaccgccat aatgccgttg aggtgaactg
    16561 gattgacccg aacaacggct gggagacggc gacagagctt gttgaagata cgcaggccat
    16621 tgcccgttac ggtcgtaatg ttacgaagat ggatgccttt ggctgtacca gccgggggca
    16681 ggcacaccgc gccgggctgt ggctgattaa aacggagctg ctggagacgc agaccgtgga
    16741 tttcagcgtg ggtgccgaag ggcttcgcca tgtgccgggt gatgttattg aaatctgcga
    16801 tgatgactat gcggggatca gcatcggcgg gcgcgtgctg gctgtgaaca gccagacccg
    16861 gacgctgacg ctcgaccgtg aaatcacgct gccatcctcc ggtaccacgc tgataagcct
    16921 ggttgacgga agtggcaatc cggtcagcgt ggaggtccag tccgtcaccg acggcgtgaa
    16981 ggtaaaagtg agccgtgttc ctgacggcgt tgccggatac agcgtatggg ggctgaagct
    17041 gccgacgctg cgccagcgcc tgttccgctg cgtgagtatc cgtgagaacg atgacggcac
    17101 gtatgccatc accgccgtgc agcatgtacc ggaaaaagag gccatcgtgg ataacggggc
    17161 gcactttgac ggcgaccaga gcggcacggt gaatggtgtc acgccgccag cggtgcagca
    17221 cctgactgcc gaagtcaccg cagacagcgg ggaatatcag gtgctggcgc gctgggacac
    17281 gccgaaggtg gtgaaggggg tgagctttat gcttcgcctg accgtggcag cggatgacgg
    17341 cagtgagcgg ctggtcagca cggcccggac gacggaaacc acataccgct tcacgcaact
    17401 ggcgctgggg aactacaggc tgacagtccg ggcggtaaat gcgtggggac agcagggcga
    17461 tccggcatcg gtatcgttcc ggattgccgc accggcagcg ccgtctcgga ttgagctgac
    17521 accgggctat tttcagataa ccgccacgcc gcatcttgcg gtttatgacc cgacggtaca
    17581 gtttgagttc tggttctcgg aaaaacggat tgctgatatc aggcaggttg aaaccacagc
    17641 ccgctatctt ggcacggcgc tgtactggat agctgccagt atcaatatca ggccgggcca
    17701 tgattattat ttttacgttc gtagtgtgaa caccgttggc aaatcggcat tcgtggaggc
    17761 tgtcggtcgg gcgagcgatg atgcggaagg ttacctggat tttttcaaag gcaagataac
    17821 cgagtctcat ctcggcaagg agttgctgga aaaagtcgac ctgacggagg ataacgccag
    17881 cagactggat gagttttcga aagagtggaa ggatgccagt gataagtgga atgccatgtg
    17941 ggctgtcaaa attgagcaga ccaaagacgg caaacattat gtcgcgggta ttggcctcag
    18001 catggaggat acagaggaag gcaaactgag tcagtttctg gttgccgcca atcgtatcgc
    18061 gtttattgac ccggcaaacg ggaatgaaac gccgatgttt gtggcgcagg gcaaccagat
    18121 attcatgacc gacgtgttcc tgaaacgcct gacggccccc accattacca gtggtggaaa
    18181 tccaccggca ttttccctga caccggacgg aaagctgact gctaaaaatg cggatatcag
    18241 tggcagtgtg aatgcgaact ccgggacgct caacaacgtc acgattaatg agaactgtca
    18301 gattaagggg aaactgtcag ccaatcagat tgaaggcgat attgtcaaaa cggtcagcaa
    18361 gtctttcccc cgcacgaaca gttatgccag tggcaccatc acggtaagaa tcagtgatga
    18421 tcagaaattt gaccggcagg tcatgatacc gccagtgtta ttccgcggtg gtaagcatga
    18481 gaatttcaac agtaataacc aacagtcata ctggtattca acctgccggt taagagtgac
    18541 ccgcaatggt caggagattt ttaatcagtc cacgacggat gctcagggcg tattttcctc
    18601 agttatagat atgcctgccg gacaggggac gctgacactg acattcaccg tatcttcatc
    18661 aggagcgaat aactggacac caacaaccag tatcagcgat ctgctggttg tggtgatgaa
    18721 aaaatccaca gcaggtatca gtatcagctg aattttataa cccagaacgg gcgtcagaaa
    18781 tgacgccttt tttattgcag aaaagcgaga ggtaattatg cgtaaacttt atgccgccat
    18841 tttgtccgca gccatttgtc tggccgtatc cggtgcgcct gcatgggcgt ctgaacatca
    18901 gtccacgctg agcgcggggt atcttcatgc ccggacgaac gttcccggca gcgatgatct
    18961 taacgggatt aacgtgaaat accgttatga gtttacggac acactggggc tggtgacgtc
    19021 attcagctat gcaggagaca agaatcgcca gcttacccgt tacagcgata cccgctggca
    19081 tgaagattcc gtgcgtaacc gctggttcag cgtgatggcg gggccgtctg tgcgcgtgaa
    19141 tgaatggttc agcgcgtatg cgatggcggg tatggcttac agccgtgttt cgactttctc
    19201 cggagattat atccgcgtaa ctgacaacaa ggggaaaaag cacgatgtgc tgaccggaag
    19261 tgatgacggt cgccacagca acacgtctct ggcgtggggg gctggcgtgc agtttaaccc
    19321 gaccgaatcc gtggccattg atattgctta tgaaggctcc ggcagtggtg actggcgcac
    19381 tgacggtttc atcgtgggtg tcggttataa attctgatta gccaggtaac acagtgttat
    19441 gacagcccgc cggttcaggc gggctttttt gtggggtgaa tatggcagta aagatttcag
    19501 gtgtactgaa agacggcaca ggaaaaccgg tacagaactg cacaatccaa ctgaaagcaa
    19561 aacgtaacag caacacggtg gtggtgaaca cgctggcctc agaaaatccg gatgaagccg
    19621 ggcgttacag tatggacgtt gagtacggtc agtacagcgt tattctgttg gtggagggat
    19681 tcccgccgtc acatgccggg accatcaccg tgtatgaaga ttctcaaccc ggtacgctga
    19741 atgattttct cggtgccatg acggaggatg atgcccgtcc ggaggcactg agacgttttg
    19801 aactgatggt ggaagaggtg gcgcgtaacg cgtccgcggt ggcacagaac acagcagccg
    19861 cgaagaagtc agccagcgat gccagcacat cagcccgtga ggcggcaacc catgcgactg
    19921 atgctgcagg ctcagcacgc gcagccagca cgtcagccgg acaggccgcg tcgtcggctc
    19981 agtcagcgtc ttccagcgca ggaacggcat cagcaaaggc cactgaagca tcaaaaagtg
    20041 ctgccgctgc agagtcctca aaaagcgcgg cggctaccag tgccggtgcg gcgaaaacgt
    20101 cagaaacgaa tgcggcagcg tcacaacaat cagccgccac ttctgcatcc accgcgacca
    20161 cgaaagcgtc agaagctgcc acctcagcca gggatgcgtc ggcttcaaaa gaggcggcaa
    20221 aatcatcaga aacgaacgca tcatcaagcg ccagtagtgc agcttcctcg gctacggcgg
    20281 caggaaattc cgcgaaggcg gcaaaaacgt ccgggacgaa cgctaagtct tctgagacgg
    20341 cagcagcaca gagtgcctcc gcagcagcag actcaaaaac agcggctgcc tcatctgcca
    20401 gcgccgcgtc aacaaatgcc gggcaggcct cagccagtgc caccgctgcc ggaaagtcgg
    20461 cagaaagcgc cgcatcatcc gcttcaacag ccacaacgaa ggctggcgaa gccactgaac
    20521 aggccacggc agcagcgagg tcagcttccg ca
//
LOCUS       DAZGVQ010000091        20240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000091 DAZGVQ010000000
VERSION     DAZGVQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.092"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(173..919)
                     /gene="cutC"
                     /locus_tag="ACHLAG_003169"
     CDS             complement(173..919)
                     /gene="cutC"
                     /locus_tag="ACHLAG_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFP0656349.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(933..1499)
                     /gene="yecM"
                     /locus_tag="ACHLAG_003170"
     CDS             complement(933..1499)
                     /gene="yecM"
                     /locus_tag="ACHLAG_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP0656350.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQFAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1715..3448
                     /gene="argS"
                     /locus_tag="ACHLAG_003171"
     CDS             1715..3448
                     /gene="argS"
                     /locus_tag="ACHLAG_003171"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFP0656351.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYESLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3490..3534)
                     /gene="yecV"
                     /locus_tag="ACHLAG_003172"
     CDS             complement(3490..3534)
                     /gene="yecV"
                     /locus_tag="ACHLAG_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFP0656352.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3625..4113
                     /gene="yecT"
                     /locus_tag="ACHLAG_003173"
     CDS             3625..4113
                     /gene="yecT"
                     /locus_tag="ACHLAG_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFP0656353.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4233..4625)
                     /gene="flhe"
                     /locus_tag="ACHLAG_003174"
     CDS             complement(4233..4625)
                     /gene="flhe"
                     /locus_tag="ACHLAG_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFP0656354.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4625..6704)
                     /gene="flhA"
                     /locus_tag="ACHLAG_003175"
                     /pseudo
     CDS             complement(4625..6704)
                     /gene="flhA"
                     /locus_tag="ACHLAG_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(6697..7845)
                     /gene="flhB"
                     /locus_tag="ACHLAG_003176"
     CDS             complement(6697..7845)
                     /gene="flhB"
                     /locus_tag="ACHLAG_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFP0656355.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8047..8691)
                     /gene="cheZ"
                     /locus_tag="ACHLAG_003177"
     CDS             complement(8047..8691)
                     /gene="cheZ"
                     /locus_tag="ACHLAG_003177"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFP0656356.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8702..9091)
                     /gene="cheY"
                     /locus_tag="ACHLAG_003178"
     CDS             complement(8702..9091)
                     /gene="cheY"
                     /locus_tag="ACHLAG_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFP0656357.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9106..10155)
                     /gene="cheB"
                     /locus_tag="ACHLAG_003179"
     CDS             complement(9106..10155)
                     /gene="cheB"
                     /locus_tag="ACHLAG_003179"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFP0656358.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10158..11018)
                     /gene="cheR"
                     /locus_tag="ACHLAG_003180"
     CDS             complement(10158..11018)
                     /gene="cheR"
                     /locus_tag="ACHLAG_003180"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFP0656359.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11037..12638)
                     /gene="tap"
                     /locus_tag="ACHLAG_003181"
     CDS             complement(11037..12638)
                     /gene="tap"
                     /locus_tag="ACHLAG_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFP0656360.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQESQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12684..14345)
                     /gene="tar"
                     /locus_tag="ACHLAG_003182"
     CDS             complement(12684..14345)
                     /gene="tar"
                     /locus_tag="ACHLAG_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFP0656361.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14490..14993)
                     /gene="cheW"
                     /locus_tag="ACHLAG_003183"
     CDS             complement(14490..14993)
                     /gene="cheW"
                     /locus_tag="ACHLAG_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFP0656362.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(15014..16977)
                     /gene="cheA"
                     /locus_tag="ACHLAG_003184"
                     /pseudo
     CDS             complement(15014..16977)
                     /gene="cheA"
                     /locus_tag="ACHLAG_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
     gene            complement(16982..17908)
                     /gene="motB"
                     /locus_tag="ACHLAG_003185"
     CDS             complement(16982..17908)
                     /gene="motB"
                     /locus_tag="ACHLAG_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFP0656363.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17905..18792)
                     /gene="motA"
                     /locus_tag="ACHLAG_003186"
     CDS             complement(17905..18792)
                     /gene="motA"
                     /locus_tag="ACHLAG_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFP0656364.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18919..19497)
                     /gene="flhC"
                     /locus_tag="ACHLAG_003187"
     CDS             complement(18919..19497)
                     /gene="flhC"
                     /locus_tag="ACHLAG_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFP0656365.1"
                     /translation="MSEKSIVQETRDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19500..19850)
                     /gene="flhD"
                     /locus_tag="ACHLAG_003188"
     CDS             complement(19500..19850)
                     /gene="flhD"
                     /locus_tag="ACHLAG_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFP0656366.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4851 a         5568 c         4928 g         4893 t
ORIGIN      
        1 gaatataaaa aatattatta gcacaacgct atgttcactg tcacccgctc aacagatcac
       61 ggttatcgtt cgttttttat actgctcagg gataaaaaaa ggcccctgtt gaaattgcag
      121 gggcctggta cgagcaagca tcatattggg cgacatgatg caacggtaaa aatcatttgg
      181 cctgatggcg ttcaatgatt cctttcattt cagcaaccgc cgccccgtct acgatataac
      241 gcgaatactc gtccgcgtgt tcatctgatg acatggacaa tccttgatta cgataacgca
      301 tcggtgaggc ttgccacgct cccgcggagc tatggacttc cagcactccg gcatcgagga
      361 agtggtgcag gttttctgca cggactcctg ctccggccat aatgattgga gcatcacgat
      421 gggcaataag ttccataatt tttgataaac cttgcagtgc gtctgatttt tgccctgatg
      481 tcagtacccg ggcaatgccc aattccgcaa gattattgag tgtatataaa gggttagcgc
      541 acatatcgaa ggcgcgatga aaagtcactg ccagcggacc ggcagcagcc attatttttt
      601 ccattcgtgg catatcgaca ttcccgtcaa catcgagaac gcccgtcacc agtccaggaa
      661 aacctaattc gcggaccgtg cgcacatcct caagaatggc ggcaaactca ccgtcgctgt
      721 agcaaaaatc accaccgcgt gggcgaatta tcggatgcac agggatcgtc acccgctggc
      781 gcacggattt cagtaccccc aacgacggcg ttaagccccc ctcttttggg gctgcgcata
      841 attcaactct gtctgcgccg ttttgctgcg ccgttagtgc acattccatg ctgtaacagc
      901 aaatttccag taatgccatt tttgctcctt aattacgctg actgctcgct ggcaacgatc
      961 tcttcaatgg accacggatg aaacttaata gttgttttgc catcggtgac cgccagcgtc
     1021 ggattcggta atcgctcatg ctcacctttt ggtgaactgg ttttaactga gatccccggt
     1081 aggctgagtc cttcatcaga aagcaatgcc agcgcacggg cattcagcgt ttctggatca
     1141 cccggcagaa cgatttcaat atgttcccaa ccttcgtgtg ggtaacgttt ttccccgggc
     1201 cacggtagct ccacaataga aaactgccag tgcgcaaact gtaccggttc atgcaattta
     1261 aacagacaaa tcggtctgcc attgatcata ttttctgaca aaagctcgcc acactgttca
     1321 aacccgcgac gccagcgttc agcagtggcg ttttgatggc aacgcaaaga aatgtgatcg
     1381 gcagtcagcg gagtgatatt caaccccaga cggagggaaa gttcatctaa tgcgtgggta
     1441 aatcgcggta aatccgatgc aatatcctgc agctcgtcga tagattgcca gttcgccata
     1501 atcactcttc gtctttcagt aaaagcgtta atttaccctg ttgccctgcg ccaaccaacc
     1561 gctgatttca cgccgcttct gatgcaatag tgaaaacggc aatacgccac gcgcacgttg
     1621 ctgacgaaaa cagccatttg cagtatactc ccgccctaat ttctttaact ggtgcgggca
     1681 atttttgctc gcttcatcaa tgtaaggtat tccggtgaat attcaggctc ttctctcaga
     1741 aaaagtccgt caggccatga ttgcggcagg cgcgcctgcg gattgcgaac cgcaggttcg
     1801 tcagtcagca aaagttcagt tcggcgacta tcaggctaac ggcatgatgg cagttgctaa
     1861 aaaactgggt atggcaccgc gacaattagc agagcaggtg ctgactcatc tggatcttaa
     1921 cggtatcgcc agcaaagttg agatcgccgg tccaggcttt atcaacattt tccttgatcc
     1981 ggcattcctg gctgaacatg ttcagcaggc gctggcgtcc gatcgtctcg gtgtttctac
     2041 gccagaaaaa cagaccattg tggttgacta ctctgcgcca aacgtggcga aagagatgca
     2101 tgtcggtcac ctgcgctcta ccattattgg tgacgcagca gtgcgtactc tggagttcct
     2161 cggtcacaaa gtgattcgcg caaaccacgt cggcgactgg ggcactcagt tcggtatgct
     2221 gattgcatgg ctggaaaagc agcagcagga aaacgccggt gaaatggagc tggctgacct
     2281 tgaaggtttc taccgcgatg cgaaaaagca ttacgacgaa gatgaagagt tcgccgagcg
     2341 tgcacgtaac tacgtggtaa aactgcaaag cggtgacgaa tatttccgcg agatgtggcg
     2401 caaactggtc gacatcacca tgacgcagaa ccagatcact tacgatcgtc tcaacgtgac
     2461 gctgacccgt gatgacgtga tgggcgaaag cctctacaac ccaatgctgc caggaattgt
     2521 ggcggatctc aaagccaaag gtctggcagt agaaagcgaa ggggcgaccg tcgtattcct
     2581 tgatgagttt aaaaacaagg aaggcgaacc gatgggcgtg atcattcaga agaaagatgg
     2641 cggctatctc tacaccacca ctgatatcgc ctgtgcgaaa tatcgttatg aatcactgca
     2701 tgccgatcgc gtgctgtatt acatcgactc ccgtcagcat caacacctaa tgcaggcatg
     2761 ggcgatcgtc cgtaaagcag gctatgtacc ggaatccgta ccgctggaac accacatgtt
     2821 cggcatgatg ctgggtaaag acggcaaacc gttcaaaacc cgcgcgggtg gtacagtgaa
     2881 actggccgat ctgctggatg aagccctgga acgtgcacgc cgtctggtgg cagaaaagaa
     2941 cccggatatg ccagctgacg agctggaaaa actggctaac gcggttggta ttggtgcggt
     3001 gaaatatgcg gatctctcca aaaaccgcac cacggactac atcttcgact gggacaacat
     3061 gctggcgttt gagggtaata ccgcgccata catgcagtat gcttacacgc gtgtattgtc
     3121 cgtgttccgt aaggcagaaa ttaacgaaga gcaactggct gcagctccgg taatcatccg
     3181 tgaagatcgt gaagcgcaac tggcagctcg cctgctgcag tttgaagaaa ccctcaccgt
     3241 ggttgcccgt gaaggcacgc cgcatgtgat gtgtgcttac ctgtacgatc tggctggtct
     3301 gttctctggc ttctacgagc actgcccgat cctcagcgca gaaaacgaag aagtgcgtaa
     3361 cagccgtctg aaactggcac aactgacagc gaagacgcta aagctgggtc tggatacgct
     3421 gggtattgag acagtagagc gtatgtaatc gatttttcgt gagagtaaag cctgatcagg
     3481 gttacctgat caggcttttt tattgccatc taaatgtatt ctgaaaatgg acatgccatt
     3541 gttttctgac tgttggataa gaggccagaa gcgtaatatc cggccccagg gaaacgataa
     3601 cggttgaatt taaggaatac cgcagtgttt aaatttcttg tattaacatt aggcattatc
     3661 tcttgccagg cttacgcaga agatacggtt atagtaaacg accatgacat ttcagccatc
     3721 aaagattgtt ggcaaaaaaa ttcagatgat gatactgaca ttaacgtgat caaatcatgc
     3781 ctgcgacaag aatacaatct cgtcgatgcg caattaaata aagcctatgg tgaagcttat
     3841 cgttatatag aacaagtgcc acgcacaggt gtaaaaaaac ctgataccga acaacttaac
     3901 ttgcttaaaa aatcacagcg agcctggctg gattttaggg acaaagaatg tgaattaatc
     3961 ctttcaaatg aggacgttca ggatttaagt gacccttatt ctgaatcaga atggctctca
     4021 tgtatgatca tacagaccaa tacgcgaact cgccagttgc agctataccg taactctgaa
     4081 gatttttatc caagcccttt gacaagagga taattcacat ctttttggca tgttttgttg
     4141 caagctattc ctgataaata attgcaacaa gacatcgagc ctttttcact gagttattaa
     4201 acatactcgc gagcgcgtaa tttttttgtc cttcagcgat aattcacaat cacttcatta
     4261 cgttgtacct ttagcggtgg aattaaccgc ccaccgcctg gcacttccca gataaatcgc
     4321 aacggttctg ctgccgctat accggaaaag gccacggtgg ttccgctctg cccctctaat
     4381 tcgacacaac gagattgcga acacaagcga acccgcagtc ctgaaggtgt cgggccgata
     4441 agctgataac gccacgctac cagcgtcatc aatcctgaag caggttgtcg cgtagaaaga
     4501 ggcgcagacg acatcgactc accgcgatga tttagcgtaa ttcctacact acttgcctgc
     4561 cacatcccct ccccggcggc ttgcaccagc agcggaaaca ataatattgc taataaggtt
     4621 ctcattattt gccgccaatt gtcgccgtca tgcggatatg tcggttatca gacagttcca
     4681 gattcgacag gaccactaac tgcggcaagc tgcggcgcag gaagcgagac aataatggtc
     4741 gcagcgcgtg gttcaccaac aatactggcg gcgcacccag catctcctga cgggatagcg
     4801 cttcctgagt ttgcgccagt aaacgatccg ccagccctgg ctccagtcct cccccgccct
     4861 gcagcgcctg tagtaacaaa cgttccagcg gtgtatcgag gccaataaca tggacttcat
     4921 ctttgccagg aaaccactgc tgggtaatcg cccgtcccaa cgccacgcgc acgacggcgg
     4981 ttaattcatg tggatcgctt tggatgggcg catgttccgc cagcgtttcg agaatggtgc
     5041 gcatatcgcg aatcggtact ttttcatcga ggatgatttt gcagcacttt atgcagtgtg
     5101 gtgagcgtga cgacgccagg aacgagatct tccgtcagct ttggcatctc ctgggcgacg
     5161 cgatccaaca gctgttgcgc ctcctgacga ccaaacagct ctgcggcatg ctggctaatg
     5221 aggtggttaa gatgcgttgc taccaccgtg ctggcctcaa ccactgtgta cccctgaatc
     5281 tgcgcctgtt cttttagcgc actttcaatc cagatagcat tcaggccaaa tgccggatcg
     5341 acggtcgcct caccaggtaa cgtcccggca gcggttccag ggttaatcgc cagccagcgc
     5401 cccggataag catcaccact gccaatctcc acgcctttca tcaaaatgcg atagcgggca
     5461 ggttgcagat ccatattgtc gcgaatgtgc accactggcg gcagaaatcc catctcctgg
     5521 gcaaatttct tgcggatact gcgtatacgg cccaacaact caccatcctg ctggaaatcg
     5581 accatcggga tcagtcgata acccacttcc attcccagag aatcttccag ttgtacatcg
     5641 ttccacgtcg cttcgacaac ggtattattc tctgccattt ttaccggttt gggttcgaca
     5701 ggcgcttttt gttcgcgtcc gcgtatccac caggccagcc ccagcaatcc ggcagtgaac
     5761 agcaaaaata ccaggttcgg cattccaggc accaggccga gtaaaccgag cacggcggcg
     5821 cttaacaaca taacgcttgg gttactgaaa agctgattca ccatctgctc gccaacatcc
     5881 tgatcggtgc tgacacgcgt aacgatgacc cccgcggcgg tagaaatcac cagcgccgga
     5941 atttgtgcca ccagaccgtc gccaatggtc aatagtgtat aactttccgc cgcgtgtccc
     6001 atgctcatgc catgttgcag cacgccgacc agcaacccgc cgacaacgtt aatgaccatg
     6061 atgaggatcc cggcgatggc atcgccgcga acaaacttac ttgccccgtc cattgagccg
     6121 taaaaatcgg cttcctgagt cacttcggag cggcgttttt tcgcctcatc ttcaccaatc
     6181 aatccagcgt taaggtcggc gtcaatcgcc atctgcttac ccggcatacc atcgagaaca
     6241 aagcgcgcac ccacttctgc gatacgcccg gcacctttgg taatgaccat aaagttgatg
     6301 atcacgagaa tgacaaacac tacgataccg atagcgaaat tgccaccaac gaggaagtga
     6361 ccgaatgctt cgaccacctt cccagccgcc gccgcgccgg tatgcccttc catcaaaatg
     6421 atacgggttg aagccacgtt aagtgccaga cgcaacaacg tagtaaacaa cagaatggtc
     6481 ggaaacgcag caaactcaag cgtgcgctgg gtaaacatcg ccaccagcaa caccatgatc
     6541 gacaaggcaa tattgaaggt aaacaacagg tcgagtatga atgcgggcag tggcagcacc
     6601 atcatcgaca agatcaacag gatcaaaatc ggtccggcaa ggatctgcca ttgtgtcgat
     6661 ttcaggtttg cgggcaggcg cagcatcgcg gccagattac tcatgggtcg gtttctcgtt
     6721 aataaaatcc agggcttccg gcaccggaag atgagtaggt tgtacagggc gctgtccacc
     6781 agccagacgc cagcgtttca gttgccagac ccaggccagc acttccgcca ccgcggcgta
     6841 cagttgaccc gggatttgtt gaccaatctc cgcatgtcga tacagcgctc gcgccagcgg
     6901 cggcgcttca agcgtcggga cgttattttc agcgccaatt tcacgaatgc gcagcgcgac
     6961 cagccctgca cctttagcga ccactttcgg tgcgctcatt ttgttttcgt catactgcaa
     7021 cgctaccgaa tagtgggtcg ggttattgac aatgacatcc gctttcggca catcggccat
     7081 catccgacgc cgtgcagcag ctcgctgcat ctgacggatc cgccctttaa catgagggtc
     7141 accttcgctt tgtttgaact catcacgaat atcctgccgt gacatacgca gctttttcag
     7201 gtggctgaat atttggaaaa agacgtcaaa tcccaccatt ggaatgacac caagcaccac
     7261 cagcagtgcg catagcccta ccaaatccat cgcattaccc atggcggtaa tcggagactc
     7321 ggccatcaag cgcatcatct gcggccagtg atgccagaga aaaaaccccg tcacgctgcc
     7381 aaccaggatg gttttcaaaa ttgctttaag caactccgcg ccagtctgag ccgagaacat
     7441 ccgtttaatg cccggtagcg ggttgagttt ggaaaacttc ggctgcaagg atttgccgct
     7501 aaataccagc cctcccagca tgaccggaga aatgagcgcc accagcacca cgccgctaat
     7561 cagcggcagc agcgccagca tggcttctct gatcagcaga ataatctgcc cgaggatcag
     7621 attcgggtca ttgataatac tgtgatcaaa atgcagccca gcggagagca tgcccgacaa
     7681 tcgacgggcc agcgacacac cgccaaacca gataacacta acgcccacca gcaaaatcag
     7741 cagtgaggtc agttcacggg aacgcgggat ttgcccctct tcccgcgctt tttctagtcg
     7801 gtgaggtgtg ggggcttctg ttttgtcgtc gctctcgtca gacacgtcgc caatcctgga
     7861 aagagtatta agcgtgaatg atgccagagc gcaaagcgtt caatggtttg agtaaggggc
     7921 aaaacaggcg ggatttaggg cttttgctgc cacacatcaa gcatagtgtg cgtttgtcgg
     7981 atgcggcgtc atcgccttat cagaccgcct gatatgacgt ggtcacgcca catcaggcaa
     8041 tacaaatcaa aatccaagac tatccaacaa atcgtccacc tgatcctgac tggctaccac
     8101 accggctttg ctggtatcga cctgaggtcc attaagcaaa ctctggtttt cacgttttgg
     8161 acgcgactcc tgttccggga tgttttccaa cagcaccatc agcaactggc gttcgatctc
     8221 ctgaatgaca tccatcatcc gcttaatgac ctgcccggtg agatcctgaa aatcctgcgc
     8281 catcatgatt tccagcagtt gcgcgttagt aaagctggta tgcgcgggta catctgccag
     8341 aaattgtcgt gtatctgtta ccagttcacg ggcgtcggca aggtcaatcg gatcggcaaa
     8401 ccagtcatcc caacgttggg ttaacgcttt tgctgatttc tccatttgat cctgatgcgg
     8461 ttgtgacgcc tcaacactgt tcagcgcccg ctccgcagcc tgggcggtca tctgcacaac
     8521 atagtacaaa cgatcgcgcg catcggggat ggcttccgcc gcttcggcaa tggcctgatc
     8581 cagccccagt tcccgcaaac tgtcgcgcag catacgcgtc aggctgccga tgcgcgcaat
     8641 gatatcgcca gctgaatgct cgtcagcagg tttgattgat ggttgcatca tagtcgcatc
     8701 ctcacatgcc cagtttctca aagattttgt tgagtttttc ctccagcgtc gcggcggtaa
     8761 atggcttcac cacatagcca ctggcccccg cttgcgccgc agcaatgatg ttctctttct
     8821 tcgcttctgc agtcaccatt aacactggca atgccgacat cgcgccatcc gcacgaattg
     8881 ttttcagcaa ttccaggcca tccatattgg gcatgttcca gtcggagata acaaatccat
     8941 aaccgcctgc ctgcaactta ttgagagcgt cgacgccatc ttccgcttcc tcaacattat
     9001 tgaatcccag ctctttcagc aggttacgca ctatgcgtcg catggtggaa aagtcatcca
     9061 caaccaaaaa tttaagttct ttatccgcca tttcacactc ctgatttaaa tacgtatcgc
     9121 ctgtccggca ctaatttttg ccaacatttg ctggcttacc tggctaagat cgaccacttc
     9181 gcagacacca cccatattga tggcctcgcg cggcatgccg aacaccacgc aacttgcttc
     9241 gttttgcgca agggtccatg cccccgcctg acgcatcgcc aacattcccg ccgcgccgtc
     9301 gttgcccata ccggtcagga tcaccccaac cgcattacgc cccgcctgtt tggcgacaga
     9361 atggaacaac acatctaccg aaggccgatg acggttaacc gccgggccat cgtgaatttt
     9421 gatttggtaa tttgcgccac tacgcgacag ctccatatgc cgatcgcccg gcgcgatata
     9481 ggcatgtccc ggcaaaacac gttctccgtc ttcggcttct ttaaccccga tctgacaaag
     9541 cttattaagt ctgtcggcaa aagagcgggt gaaaccaggc ggcatatgct gggtaattaa
     9601 cagtgccggg ctggaaagcg gcaacggttg cagtacgtga cgaattgcct cagttccacc
     9661 cgttgaagca ccaatcgcaa tcagtttttc agaactcaac aacggccctg ccttcagcgt
     9721 tgtcggtgcc gacaatggct tatgtgctgc aaggctcgcc tttgctgctg tacgcacctt
     9781 ttcagcaatc atttcgctat acgccagcat tccttcgcga atacccagtt gcggtttggt
     9841 gacaaaatct atcgccccca gctccagcgc gcgcagcgtg acttctgacc ctttgccggt
     9901 caaggaagaa accatcacaa cgggcattgg acgcaaacgc attaattttt cgaggaaatc
     9961 cagtccgtcc atccgcggca tttcaacatc cagcgtcagc acatcgggat tgaatttctt
    10021 aatcaagtca cgcgcgacca gcggatcagg cgcggtcgcc accatttcca tgtcgctatg
    10081 gctgttgatg atttctgtca tgatctggcg catcagtgcc gaatcatcga cagataacac
    10141 cctgattttg ctcatcgtta atccttactt agcgcataca ccgtctgacc acgcagcgtg
    10201 aagcggcgct caaggtggct aaagttttca gagtgacccg caaacagcaa tccgtcgggt
    10261 ttaaggagcg gaacaaagcg gcgcaaaatc tcctgctggg tattttgatc gaagtagatc
    10321 atgacgttac gacagaagat cgcatcaaac ggccccggca cggtgtactg tttcgccagt
    10381 agattcagcg gggcaaaatc aacatagttc gccagctcct gacgcacgcg taccagccct
    10441 tcatgcggtc ccgtccctcg catgaaatac cgttgcagtt gctgcggcgt caggtttttc
    10501 aactcttcat ggcgataaat accgcttctg gctttttcca gcacttcggt gtcgatatca
    10561 ctggcaaaca ctttccagcg tccgggcgcg gtgcccaatg tgtcagccag cgtcatcgca
    10621 atgctgtacg gctcttcgcc ggtcgaagcc gccgcgctcc atacgcgata ctcgccagaa
    10681 cggcgacgtg cgtgatccgc gagcagaggg aaatggtgtg cctcacggaa aaatgccgtc
    10741 agattcgtgg tcagcgaatt gataaacgcc tgccactcac cgctgtgctg attagattcc
    10801 agcaagttca gataatgacc gaaatccgtc agtcccagcg aacgcaaacg acgaaccagt
    10861 cggttgtaaa ccatgtcgcg tttatggtca gccagaacga tcccggctcg ttgatagatc
    10921 aattgactta tccgccgaaa atgcgcgtcg gaaagcgcca ggcgctcggt catctgtaac
    10981 aataaagacg tttgcccaca gggcagagat gaagtcatag cgccttctca atcacttcag
    11041 gataccactg gcgcaatttg taactgcgcc gactcatgtc gtgctacttc atgttcttca
    11101 agggtaaata ccgccacacg cgacgaaaga tggtcggcct gattcgccag ttgttccgtt
    11161 gccaccgccg cttcttctac cagcgaggcg ttctgctgag tcacctgatc catctggctg
    11221 acagcctgtg caacctgctc aatcccccgc tgttgttctt ccgacgcaga ggcaatttct
    11281 cccataatgt cgttcacgcg ggtcaccgaa ctgacaatat cgatcatggt cgcggcggcg
    11341 ttattcacca gtttcgaacc ctgctggaca cgattgactg actcttcgat cagccctttg
    11401 atctcttttg ctgcctgcgc gctacggctg gcaagattgc gcacttcacc tgccactacc
    11461 gcaaaaccac gcccctgctc tccggcgcga gccgcttcca ctgccgcatt cagggccaga
    11521 atattggtct ggaaagcaat tccgtcgata acgctgataa tgtcgccaat tttttgcgag
    11581 ctggtggcga tctcctgcat ggtgtgagtc atggtactga cctgaacacc gcccgcctgc
    11641 gccgttgtcg cggcattttt tgccagttcc gacgcctgtc gtgcgttatc ggcgttttgc
    11701 cctaccgtgg cggttaattg ctccatactg gcggccgttt gtgccagcga tgccgcctgc
    11761 tgttcggtac gacttgagag atcgttattg cctgcgacaa tctccgcgat accaatgtgc
    11821 atctcctggc ttcccttacg cacatcactt accgtcccac gcaaagcctg ctgcatggtc
    11881 ttcagactgg caaaaatggc ggtgatctca ttacgaccat ataccgcaat cggacgcgcc
    11941 agattacccg cagcaatgct gtcaaaatgg ctaccgataa tggccagtgg ttgaacaatc
    12001 atcttgcgcg tccaccacag cgcactgctg atgtagatcg ccgcaacaat aatcatgctg
    12061 ataaacacca gcgccgaaat ctgatagtta cgctggcttt gcgcactggc ggcttccagc
    12121 acatggttga tctccagctg ccaggcctca aagttaacgt caaacgcatt ctgtgattcc
    12181 tgcaccggcg cagtgaggaa atccgaaagt tgattacttt caagccaggt cgcctgatgt
    12241 tcgagatcgt tgtgccagcg ggcaaaactt ttctccgtct ctttttgcaa cgccctgacg
    12301 tgctcgttgc ccgcagtcat cgccataaaa cttttaaaca gcgtggtgga ttgcgtcaga
    12361 ctggcgcgcg ccgtcgtcat caacgtttta atgtcatccg ccggatagct aagcgccgtc
    12421 agagtgcccg ctttgttcag cgcggtactg gcctgtaaca ttaccgcccg agtttgcgct
    12481 aatgccgcac gttgctgatt gctctgctcg acctgattca gtcgttgcaa atcgtcgcga
    12541 aatgcccaaa aagacatgcc gttactgcca atctgcaaga tcccgcagag aatcaaaatt
    12601 aaaaacagcg tggtcgaaat tcgaatacga ttaaacatca acgctcccca tcaggcggca
    12661 atgaccgcgt tagtaaatac tcgtcaaaat gtttcccagt ttggatcttg ttcagcaatt
    12721 cgcagtcgtg gctgtgccgg tggttgctca ctggcaggac gggatggtgt ttgcggttta
    12781 ttggtgagtg ggctggctgc cagacggaac gcggaaaccg cttgcgttaa acgactcgcc
    12841 tgttcttcca gcgcagcggc ggcggcagct gattcctgca ccagcgatgc gttctgttgc
    12901 gtgacgcgat ccatttccga aaccgccaat gcgacttgat cgatgccacg gctctgttca
    12961 tccgatgccg atgcaatctc gcccataatg tcagtcacgc gagtgacagc attgacgata
    13021 ttgttcattg tttccccggc gctttcgacc agcaccgaac cggtatcaac gcgtgagacg
    13081 gagtcttcaa tgagggcttt gatctctttt gccgcctggg cgctacgact ggcaagatta
    13141 cgcacttcac ccgccaccac ggcaaaacca cggccctgtt cacccgcacg cgcggcttca
    13201 accgcggcat tcagcgcgag gatattagtc tggaaggcaa taccgtcgat aacgctgata
    13261 atgtcggcaa ttttcttcga actatcggcg atctcatgca tcgttttcac tacgccatcc
    13321 accactttgc cgccgtgctg ggcggtgtcg gaggcacttt gcgccagttg cgaggcctgg
    13381 cgggcgttat cggcgttttg cttcactgtc gcggtgagct gctccatgct ggcggcagtt
    13441 tcttccagcg cggatgcctg ctgttcagta cgggaggaaa gatcggtgtt gcccgccgca
    13501 atttcacggg taccggcata gatggcatct gaaccttcgc ggacatgagt gacggtgtca
    13561 gtcaaagagc gttgcatatg tgaaacgctc tgcgccaggt cgcccatttc actgcgcccg
    13621 tcaatggtca gggtattcgc caggttacca ccggcgattt cgcgaatgtg agcaataatt
    13681 tttgccagcg gagtaagcaa catacggcga atgccgtacc acgccaccag cagaatcaat
    13741 accaccacca gcgcgataac cgccagttgc cactgggcaa atcggtaatc atctgcgttg
    13801 tcagtgacga tatcgcgata cagtttttca ctgctgaggg cgtactgagc aaacgcttcg
    13861 cccattgcat tttgcattcc ctgggttggc tgagcgaaat aagctccagt attgccataa
    13921 tctagataat caatcagttc agttaacgct gtgtaatagt ttttatattt ttcatcaata
    13981 ttacgactgg tagcgaccat ttcaggtaac ggtgccatgc ttttgaattt tttataatgc
    14041 gtcgctgcct gcgccaatgt tttcctggcg ctatcgagca attcaacttt ggcgttactt
    14101 tgttgattgg aggaatccat catcatccgt accgctgaac gactcaggtt aatgcgcgtt
    14161 tgcagcatta aatcccaggt tgacgtcagc tcgccctgct gttcccgtaa ttgattggaa
    14221 accacaaagc tcttctggct atggtgaagg gaagaaaaaa acagactgcc ggaaataagc
    14281 tgtaacagtg cgaatacccc cagcaccatt accaacagcg tgactacgcg gatacggtta
    14341 atcataaggc accttcctga aaacaagttg atctcgttat cggcaaggag gggggaaact
    14401 ttattgctga tgccacccgc cgcgaaattg aaataaaaaa cccgatgcgc agatcatcgg
    14461 gttcatttca attgaggaaa tcgggagaat tacgccactt ctgacgccgc gctatctaac
    14521 agcgccatct cttcgctgtt cagcagtttt tcgatgttca ccagaatcaa catccggtcg
    14581 cccagtgcgc ccagtccagt gagatattct gttgaaagcg tcacggcaaa ttccggtgcc
    14641 ggacgaattt gctccgccgt caatgaaagc acgtctgaga cgccgtcaac cacgatgccg
    14701 accacccgct gtccgagatt caggacgata actaccgtgt tgtcgttata gtccacatcc
    14761 acctggctga acttaattcg taagtcaaca atcggcacaa taaccccgcg cagattcgtg
    14821 acgcctttga taaacgctgg cgtgttcgca atccgtgtta cctgatcgta gccacggatc
    14881 tcctgcactt tcaggatatc aataccgtac tcttcatcac caagggtaaa taccagaaat
    14941 tcctggcctg acggctcgct ggccagcttt gttacattcg tcataccggt catattgtta
    15001 cctttttact cattcaggcg gcggtgttcg ccatacgttg ttcgcggttt atcgcctgca
    15061 aggcggagac atcaacaatc agtgccacgc tgccgtcgcc aagaatggtc gcagcagaaa
    15121 tgccggggac tttgcgatag ttactttcaa ggtttttaac cacaacctgg tgctgaccaa
    15181 ttaattgatc caccagcaag gcgtagcggc gaccgccact ttgtaagatc accacaattc
    15241 cctgggtggc ttcggttttc gcgcccgcga cgttgaacac tttccacagt tcgacgatgg
    15301 gcagatattc accccgcact tccagcaccc gctcgccgcc ggccagtgga tgcagatcgg
    15361 cttcacgggg ttgcagtgat tccataacag cattcagcgg cagaatgaaa acttcatccg
    15421 caacgcgtac ggacatgccg tcgaggatgg ccagcgtcag cggcagtaaa atgcggatcg
    15481 tagtgccagt accctgcttc gactggattt cgacatgacc gcccatctcc tggatattac
    15541 gtttaacgac gtccatgccg acgccgcgcc cggagacgtc ggtgacctgc tctgccgtgg
    15601 agaagcctgg tgcaaatatc agcatcgcga cttcgtcgtc gctcatgttt tcgctgacag
    15661 tcaaaccttg cgaggccgct tttgccagaa ttcgctcacg gtttagcccc gccccatcgt
    15721 cggtcacttc aatgcaaatg ttgccgccct gatgttcggc agacagaatt aaatttccga
    15781 cgctgttttt acctgcggcg agccgttttt ctggcagttc aataccgtga tcgaggctat
    15841 tgcgtaccag gtgggtcagc gggtcgataa tgcgttctat caggctcttg tcgagctcgg
    15901 tggaactgcc caccagcgtc agttctacct gcttgccgag ttttcccgcc agatcacgca
    15961 ccagccgggg atagcgacta aagacatatt ccatcggcat catgcgaatc gacatcactg
    16021 attcctgcaa atcacgggcg ttacgttgta actgccccat gctggtaatc aaatcaccat
    16081 gattaaccgg gtccagttcg ctggaacgct gggcaagcat ggactgggtg ataaccagct
    16141 cgccgacgag gttaattaat tgatcaacct tttctaccgc tacacggatg ctggtggatt
    16201 cactgctgcg cgtcgttttt tcccgctcca cgcgaccgtt tgcgcttgtt cggctgccag
    16261 tttaagcact ggtggggtgg atatttttgg cgagacttct actgtttcaa aggtaatctg
    16321 atcggcttca atcacaaaac agagtaccgc tgtgatgtca tcttcggcga tatcgcccgg
    16381 taatattgcc gagagagaat ccgccccttt caccacgtca gttaacgttg tcagatgccc
    16441 cagctcttct tccagcaggt cgacttcgcc ggccttcagg cgcgaaagga taattcgtcg
    16501 cggcgactga ctgcgactct gctcatcttg cggttcactt ttggcaacca cacttaatcg
    16561 ggtcactgcg gatggcgttt cgcctttcgc ttctaatgcc agttgacgca aggcctggca
    16621 gatataatcg aagctggcgg catccggctc ttgcgactgt ttataagcgt cgagctgttc
    16681 ttgcatgatg tccttcgttt ccaaaaacag attgataatg tcggtgttga gttgcatctc
    16741 acctcgtctg gcttcatcga gcaggttttc catcagatgc gtggtttcct gcaaaacgct
    16801 gaagccaaaa gttcctgccc ctcctttgat cgagtgggca gcccgaaaga tggcattcaa
    16861 ttgttcggca tctggcgctt ccggctgcaa aaccagcaga tgctgctcca tatcagccaa
    16921 cagttcgtcc gcttcatcaa aaaatgtctg ataaaaatcg cttatatcca tgctcacgct
    16981 gtcacctcgg ttcggctgat ggcattgtgg gaacactgac ctgtggtgca acctcaggtt
    17041 tttccagggc gcttactggc tcattctggc tttcggcgtt ttcatgcaaa atggcctgtt
    17101 cggcttgttt gttcagtacc agcaggctga tgcgacggtt gacggcatca tcaggtccgc
    17161 gatcgcttaa gcgcatcgtt gccgccatgc cgacgacacg taacactttg ccgctatcca
    17221 accctccgac catcagttcg cggcgggatg cattggcccg atcggcagaa agctcccagt
    17281 tgctatatcc tttctcaccg ctggcgtagg ggaaatcatc ggtatgacct gaaaggctaa
    17341 tacggttggg aataccgttc agtacaggcg caatggcacg cagaatgtcg cgcatatagg
    17401 gttcgacatc ggcactgccg gttctaaaca tcgggcgatt ctggctatcg atgatctgaa
    17461 tacgtagacc ttcctggacc agatcgattt tgagatgggg acgtaacgcc cgcagtttcg
    17521 gatcggactc tatcaactgg tcgagatcac cccgcaattt ccgcaatcga ctttgctcca
    17581 tgcgtttttt cagctcttcg atgttcggct gcttattcac ttccccctgg ctttgggtgt
    17641 aatcatcacc accgccggga attgggcttt cactattaga aatgcgatcg ccgcccgtaa
    17701 ccgcagtcgc cagtggagtc cggaagtact ccgcaatctg aatcagctct tttgggctgg
    17761 agatggagat cagccacatc accagaaaaa aggccatcat cgcagtcata aagtcggcat
    17821 aagcaatctt ccacgatcca tgtgctgccc cgtggctttt ggctttgcgt cgtttgacga
    17881 caataatcgg atgcgcttga ttcttcatgc ttcctcggtt gtcgtctgtt gttgcggatt
    17941 tttcaccgca cgcacatgct cttccagttc aataaacgac ggacgttcgc tggaatagag
    18001 cgttttgcga ccaaactcaa cggcgatagg cggtgcgtaa ccgttcagat tagaaagcag
    18061 agtgactttg acgcactgca tcattttgct ggtttcggcg cttttctgac gtaaaacact
    18121 cgctaatggg gaaataaatc cgtaagccaa taaaatgccg aggaaagtcc ccaccatcgc
    18181 atgtgcgata agcgcaccca gctcggcggc aggacgatcg gctgaaccta acgcgtgaac
    18241 gacccccatt acagccgcga caataccaaa cgccggaagt gagtccccga ccagcgccag
    18301 actgttcgcc gggacttctg cctcgctttc gtgcgtctca atctcttcat ccatcagggc
    18361 ttcgatttcg aaggtgttca tatgaccgct gataatcaga cgcagataat cgacgataaa
    18421 atcaagcatg acgctatccg cgaggatgcg tgggtagctg gcgaagatct cgctctcacg
    18481 gggattttca atatcacgtt ccagcgaaaa catccccatc tgccgcgatt tcgccatcaa
    18541 ccgataaagc agagccagca gatccatata cattgctttg gtgtatttgg agcgacgaaa
    18601 cagcaacggc agcgccttca gcgtgccttt aatcgctttg ccattattgc cgacgataaa
    18661 cgacccaatc cctgcaccgg caataatcac cagttcagcg ggttgataga gtgctccaag
    18721 gcttccaccg gtcatcaaat aaccgccgaa aactgtaccg agaacaacca ggtaacctaa
    18781 taagataagc acgacatcat ccttccactg ttgaccatga caggatgttc agtcgtcagg
    18841 cgttaacgcg cgattggggc aaaaaaaagc agcggtacgt cgttaccgct gctggaatgt
    18901 tgcgcctcac cgtatcagtt aaacagcctg tactctctgt tcatccagca gttgtgggat
    18961 aatatcggca ggattctggg aaagtttacg tctttttact gcccgggatg gcggttgaca
    19021 taagctgcag gcaaagctgc caacaggctg gtgagcgtgg gtaataaaat tgccgccgca
    19081 gcagttgcag ctggaaagtt gcagtaatcc actttcaaca aaccgcacca atgtccaggc
    19141 acgggttaat gccagcagtg gtccttcttc tgcttgtggg cactgttcaa ggtataaacg
    19201 gtaggctttg atcaccgcat cgacgccatt acacaaaccg gttttcagta aaaactgcca
    19261 tgcattacag aacatcgaag catgaacgtt ttgttcccag gtcataaacc agtcggttga
    19321 gaatggcagc atgcctttcg gcggtgggct tccgcgcagt tctttataaa gttttatcag
    19381 gcgtccgcga cttaactgtg tttcgctttc cagcatctgc aaacgagctc ccagggtgat
    19441 caattccatt gccagctgaa tatcccgcgt ttcctgaaca atgctttttt cactcatgat
    19501 caggcccttt tcttgcgcag cgcttcttca ggctgattaa catcattcag caagcgtgtt
    19561 gagagcatga tgccggtatg aatttgctgg agatcgtcaa cgcgggaatc ttgcgtcaac
    19621 tgagtaatcg tctggtggct gtcaaaacgg aagtgacaaa ccagttgatt ggtttctgcc
    19681 agcttaacca tttgcggaag agtcagtgcc gctaacgttg tcgccatttc ttcatttatg
    19741 ccgagacgaa acatagcgga cgctttgtcc tgaacaatca aacgctgtgc aagtagtaaa
    19801 tatgacaagt tgatgtcata aatgtgtttc agcaactcgg aggtatgcat tattcccacc
    19861 cagaataacc aactttattt ttatgcggtt tcaccgcacc ccgtgatgtc gccgggaagc
    19921 cccggtaaaa aataattagc attagaatag ttgcgataag ctgcaataag cagaaccacc
    19981 tttttggttt aatatgtcct tacaaataga aatgggtctt tacacttatc taagattttt
    20041 cctaaatcga cgcaactgta ctcgtcacta cacgcacata caacggaggg gggctgcgat
    20101 tttcaataat gtgtgatgca gatcacacaa aacactcaat tacttaacat aaatgtataa
    20161 gtcatacttt tgttttgggt gtatttcaat ctgttaaaaa gtttttcgct acgctagcaa
    20221 gcaaaaatga aacaggaata
//
LOCUS       DAZGVQ010000092        20197 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000092 DAZGVQ010000000
VERSION     DAZGVQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20197
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.093"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..874
                     /gene="yieE"
                     /locus_tag="ACHLAG_003189"
     CDS             <1..874
                     /gene="yieE"
                     /locus_tag="ACHLAG_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFP0656367.1"
                     /translation="TLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQLGV
                     QLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNYILP
                     AVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLEDEL
                     VVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMELGNS
                     EAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLSNAL
                     RRFLDYCDPANVPR"
     gene            1079..2548
                     /gene="lysP"
                     /locus_tag="ACHLAG_003190"
     CDS             1079..2548
                     /gene="lysP"
                     /locus_tag="ACHLAG_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFP0656368.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2841..4827
                     /gene="cirA"
                     /locus_tag="ACHLAG_003191"
                     /pseudo
     CDS             2841..4827
                     /gene="cirA"
                     /locus_tag="ACHLAG_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(4859..5695)
                     /gene="yeiG"
                     /locus_tag="ACHLAG_003192"
     CDS             complement(4859..5695)
                     /gene="yeiG"
                     /locus_tag="ACHLAG_003192"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP0656369.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5953..6621
                     /gene="folE"
                     /locus_tag="ACHLAG_003193"
     CDS             5953..6621
                     /gene="folE"
                     /locus_tag="ACHLAG_003193"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFP0656370.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6638..7795
                     /gene="yeiB"
                     /locus_tag="ACHLAG_003194"
     CDS             6638..7795
                     /gene="yeiB"
                     /locus_tag="ACHLAG_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFP0656371.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            7937..8977
                     /gene="galS"
                     /locus_tag="ACHLAG_003195"
     CDS             7937..8977
                     /gene="galS"
                     /locus_tag="ACHLAG_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFP0656372.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMTKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            9257..10255
                     /gene="mglB"
                     /locus_tag="ACHLAG_003196"
     CDS             9257..10255
                     /gene="mglB"
                     /locus_tag="ACHLAG_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFP0656373.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10316..11836
                     /gene="mglA"
                     /locus_tag="ACHLAG_003197"
     CDS             10316..11836
                     /gene="mglA"
                     /locus_tag="ACHLAG_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFP0656374.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11852..12862
                     /gene="mglC"
                     /locus_tag="ACHLAG_003198"
     CDS             11852..12862
                     /gene="mglC"
                     /locus_tag="ACHLAG_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFP0656375.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(12933..14168)
                     /gene="preA"
                     /locus_tag="ACHLAG_003199"
     CDS             complement(12933..14168)
                     /gene="preA"
                     /locus_tag="ACHLAG_003199"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFP0656376.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(14162..15400)
                     /gene="preT"
                     /locus_tag="ACHLAG_003200"
     CDS             complement(14162..15400)
                     /gene="preT"
                     /locus_tag="ACHLAG_003200"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFP0656377.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQCARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(15719..15958)
                     /locus_tag="ACHLAG_003201"
     CDS             complement(15719..15958)
                     /locus_tag="ACHLAG_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFP0656378.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(15961..16680)
                     /gene="sanA"
                     /locus_tag="ACHLAG_003202"
     CDS             complement(15961..16680)
                     /gene="sanA"
                     /locus_tag="ACHLAG_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFP0656379.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(16830..17714)
                     /gene="cdd"
                     /locus_tag="ACHLAG_003203"
     CDS             complement(16830..17714)
                     /gene="cdd"
                     /locus_tag="ACHLAG_003203"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFP0656380.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(17844..18539)
                     /locus_tag="ACHLAG_003204"
     CDS             complement(17844..18539)
                     /locus_tag="ACHLAG_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFP0656381.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(18536..18934)
                     /locus_tag="ACHLAG_003205"
     CDS             complement(18536..18934)
                     /locus_tag="ACHLAG_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP0656382.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            19173..20120
                     /gene="dusC"
                     /locus_tag="ACHLAG_003206"
     CDS             19173..20120
                     /gene="dusC"
                     /locus_tag="ACHLAG_003206"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFP0656383.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRRALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
BASE COUNT         4934 a         4995 c         5271 g         4997 t
ORIGIN      
        1 caccctccgg cagttggaag tttttgcaga agtattgaaa agtggatcaa ccacccaggc
       61 gtcggtgatg ctggcgttgt cgcaatcagc agtgagcgca gccttgaccg acctggaagg
      121 gcagcttggc gtgcaactgt ttgatcgcgt ggggaaaaga ctggttgtta atgaacacgg
      181 gcggctgctc tatccgcgtg cgttggcatt gcttgaacag gcggttgaaa tcgaacaact
      241 gtttcgcgaa gacaacggcg cgattcgtat ctatgccagt agtaccatcg gtaactacat
      301 tctgcctgca gttatcgccc gttatcgcca tgattatccg cagttgccga ttgaacttag
      361 cgttgggaac agccaggacg tgatgcaagc ggtgctggat ttccgcgttg atattggttt
      421 tattgaagga ccgtgccaca gcactgaaat tatttctgaa ccgtggctgg aagacgagct
      481 ggtggttttc gccgcgccga cttcgccgtt ggcccgtggt ccggtcacct tagagcagct
      541 ggccgctgcg ccgtggatcc tgcgtgaacg cggttccggc acgcgggaga ttgtcgatta
      601 tctattgctg tcacatttac cgaagtttga gatggcgatg gaattaggta actccgaggc
      661 aatcaaacat gcggtgcgtc atgggttggg aattagttgc ctgtcgcgac gggtgattga
      721 agatcaattg caggcaggca cattaagtga agttgcggtc cctctgccgc gcctgatgcg
      781 tacgttgtgg cgtatacatc atcggcaaaa acacctttcc aacgcgctac ggcgctttct
      841 ggactattgc gatcccgcga atgtaccgcg ttaagttgct gtacaagaac atgctggtgc
      901 tgtgtcgatt tcgtgacgca gcgccctcag catgcattcg ccagaaaaga gattggctgc
      961 tttacttata atccctgggc gatcatgaag gtgtcttata accgtgtatt tctgccggaa
     1021 ggattgccaa tcgtctgcta caatcgcgcc tcatttttaa gatggatagc atttttgtat
     1081 ggtttccgaa actaaaacca cagaagcgcc gggcttacgc cgtgaattaa aggcgcgtca
     1141 cctgacgatg attgccattg gcggttccat cggtacaggt ctttttgttg cctctggcgc
     1201 aacgatttct caggcaggtc cgggcggggc attgctctcg tatatgctga ttggcctgat
     1261 ggtttacttc ctgatgacca gtctcggtga actggctgca tatatgccgg tttccggttc
     1321 gtttgccact tacggtcaga actatgttga agaaggcttt ggcttcgcgc tgggctggaa
     1381 ctactggtac aactgggcgg tgactatcgc cgttgacctg gttgcagctc agctggtcat
     1441 gagctggtgg ttcccggata caccgggctg gatctggagt gcgttgttcc tcggcgttat
     1501 cttcctgctg aactacatct cagttcgtgg ctttggtgaa gcggaatact ggttctcact
     1561 gatcaaagtc acgacagtta ttgtctttat catcgttggc gtgctgatga ttatcggtat
     1621 cttcaaaggc gcgcagcctg cgggctggag caactggaca atcggcgaag cgccgtttgc
     1681 tggtggtttt gcggcgatga tcggcgtagc tatgattgtc ggcttctctt tccagggaac
     1741 cgagctgatc ggtattgctg caggcgagtc cgaagatccg gcgaaaaaca ttccacgcgc
     1801 ggtacgtcag gtgttctggc gaatcctgtt gttctatgtg ttcgcgatcc tgattatcag
     1861 cctgattatt ccgtacaccg atccgagcct gctgcgtaac gatgttaaag acatcagcgt
     1921 tagtccgttc accctggtgt tccagcacgc gggtctgctc tctgcggcgg cggtgatgaa
     1981 cgcagttatt ctgacggcgg tgctgtcagc gggtaactcc ggtatgtatg cgtctactcg
     2041 tatgctgtac accctggcgt gtgacggtaa agcgccgcgc attttcgcta aactgtcgcg
     2101 tggtggcgtg ccgcgtaatg cgctgtatgc gacgacggtg attgccggtc tgtgcttcct
     2161 gacctccatg tttggcaacc agacggtata cctgtggctg ctgaacacct ccgggatgac
     2221 gggttttatc gcctggctgg ggattgccat tagccactat cgcttccgtc gcggttacgt
     2281 attgcaggga cacgacatta acgatctgcc gtaccgttca ggtttcttcc cactggggcc
     2341 gatcttcgca ttcattctgt gtctgattat cactttgggc cagaactacg aagcgttcct
     2401 gaaagatacc attgactggg gcggcgtagc ggcaacgtat attggtatcc cgctgttcct
     2461 gattatttgg ttcggctaca agctgattaa aggaactcac ttcgtacgtt acagcgaaat
     2521 gaagttcccg cagaacgata agaaataagt ttcctccctt ccttgctaag ccctctcaac
     2581 cgagagggct ttttcaattc catttccctg acaaatcatg cggatataaa atttaacatt
     2641 tggattgata attgttatcg tttgcattat cgttacgccg caatcaaaaa aggctgacaa
     2701 atcagaggct gttccggctt tctgggatga tcacctgcat acaaataagt ccaccgcgat
     2761 gctgccgtac gcaaggggac gtgaagaaga tgtgagcgat aacccatttt attttcgtag
     2821 ttacctcatg gagatatgga atgtttaggt tgaacccttt cgtacgggtc gggctgtgtt
     2881 tgtccgctat ttcttgtgca tggcctgtgt tagcggtcga tgatgatggc gaaacgatgg
     2941 ttgtcactgc atcttccgtt gaacaaaacc tcaaagatgc acccgccagt atcagcgtca
     3001 ttacccagga agacctgcag cgaaaaccgg tacagaatct gaaggatgtc ctcaaagaag
     3061 tgcctggcgt acaactgacg aacgaagggg ataaccgtaa gggcgttagt attcgtggtc
     3121 tggacagcag ctacaccctg attctcgtcg acggtaaacg cgttaactcc cgcaatgccg
     3181 tcttccgcca caatgatttc gatctgaact ggatcccggt cgattccatc gaacgtattg
     3241 aagtggttcg tggcccgatg tcgtcgctat acggctccga tgcgctcggc ggtgtagtga
     3301 atatcatcac caaaaaaatc ggtcagaaat ggtcgggtac cgttaccgtc gataccacca
     3361 ttcaggaaca tcgcgatcgc ggtgatacct ataacggtca gttctttacc agtggaccat
     3421 taattgatgg tgtgctggga atgaaagctt acggcagcct ggcaaaacgt gaaaaggatg
     3481 acccgcaaaa ctcaacgacc accgataccg gagaaacgcc gcgtattgaa ggattctcca
     3541 gccgcgacgg caatgtcgaa tttgcctgga caccgaatca aaatcacgat tttactgccg
     3601 gatacggttt cgaccgtcag gatcgtgatt ccgactcgct ggacaaaaac cgcctggaac
     3661 gccagaacta ctccgtcagc cataatgggc gttgggatta cggcaccagc gaactgaaat
     3721 actacggtga gaaagtcgag aacaaaaacc ctggcaacag cagcccgata acttccgaaa
     3781 gcaatacggt cgacggcaaa tacacgttgc cgctgacggc gattaatcag tttctcacgg
     3841 ttggcggtga atggcgtcac gacaaactta gcgatgcggt gaacctgacc gggggaacca
     3901 gctccaaaac gtctgccagc cagtacgcgc tgtttgtgga agatgaatgg cggatcttcg
     3961 agccgctggc gctgacgacc ggcgtgcgta tggacgatca cgaaacctac ggtgaacact
     4021 ggagtccgcg tgcctacctg gtttataacg ccaccgacac cgtaacggtg aaagggggct
     4081 gggcgacggc atttaaagca ccttctctgt tgcaacttag ccctgactgg acgagcaatt
     4141 cctgccgtgg cgcatgtaag attgtgggta gcccggatct gaaaccagaa accagcgaaa
     4201 gttgggagct ggggctttac tacatgggtg aagaaggctg gctggaaggg gttgaatcca
     4261 gcgttaccgt tttccgtaac gatgtgaaag atcgtatcag catcagccgt acgtctgacg
     4321 tcaacgctgc accgggctac caaaactttg ttggttttga gacgggcgct aacggacggc
     4381 gcataccggt atttagctac tacaacgtta acaaagctcg tattcagggc gtggaaaccg
     4441 aactgaaaat tccgttcaac gatgaatgga aactgtcgat caactacacc tacaacgatg
     4501 gtcgtgatgt cagcaacggc gaaaacaaac cgctatctgc cgttccatac tgctaacggt
     4561 acgctggact ggaaaccgct ggcgctggaa gactggtcat tctatgtttc tggtcactat
     4621 accgggcaga aacgcgccga cagcgcgacg gctaaaacac cgggcggtta taccatctgg
     4681 aataccggcg cggcctggca ggtgactaaa gacgtcaaac tgcgcgcagg cgtgctgaac
     4741 cttggcgaca aggatctcag tcgtgacgac tacagctata acgaagacgg acgtcgttac
     4801 tttatggcag tggattatcg cttctgatga gaagatgccc ggcgaaccgg gcggactttc
     4861 acttcagtaa atactgcgca tggaagcgca ggtgatcctc tataaaagag gcgatgaagt
     4921 agtaactgtg atcatatccc ggctgaatac gcagcgtcat cggccacgct ttctggcgcg
     4981 cggcttctgc cagtaccgca ggttgcaact ggtcggcaag aaactgatca ttatcgccct
     5041 gatcgataag cgtcgggatc gcatcctgcg cgttactggc atacatcagc gcgcaactgt
     5101 cccattccag ccatgcattt ttgtcctcac ctaaatagct gctaaacgct ttgattcccc
     5161 acgggacgct gcacggattc acaattggcg caaaggccga aacgctggtg tatttacccg
     5221 gatttttcag cgccataatc agcgcaccgt gaccacccat tgagtgaccg ctaatggcgc
     5281 agcggtcgct gacattaaat tgcgactgaa ccagcgccgg taattcatcg cgcagataat
     5341 catacatccg gtaatgcgtc gcccacggcg gttgcgtggc attaagataa aagcctgcgc
     5401 cctggcctaa atcgtagcca tcgtcgttgg caaccttttc gccgcgcggg ctggtgtctg
     5461 gcatcaccag tacaatcccc agttctgccg ccacgcgctg ggcacccgcc ttggtggtga
     5521 agttctcgtc attgcaggtt aatccggaaa gccagtacag cactggtggc ggggtgtgat
     5581 cacgaggggg tgggagaaag atactgaacg tcatcgggca gtttaaggta ctggagtcgt
     5641 gtcgccagcg ttgctgccag ccttcaaaac agcggtgctc ttcgagcatt tccatgcgtg
     5701 gctccttgtt gtgttgtttg caatttcttc atcataccgt ttattcaatg tgctgtgagt
     5761 aactttcact tccgtatttg cataacgatg ttttaacatc tgctgatgaa aggcagcggc
     5821 aattacaata attatcgctg tgaatactgg attatgtgcg ccgcctcacg cacaataatc
     5881 aggctgtaaa tcagcttaat aactttgccc ccacgcaggg cggaggcgtc acacctgcag
     5941 gagaaatcat aaatgccatc actcagtaaa gaagcggccc tggttcatga agcgttagtt
     6001 gcgcgaggac tggaaacacc gctgcgcccg cccgtgcatg aaatggataa cgaaacgcgc
     6061 aaaagcctta ttgctggtca tatgaccgaa atcatgcagc tgctgaatct cgacctggct
     6121 gatgacagtt tgatggaaac gccgcatcgc atcgctaaaa tgtatgtcga tgaaattttc
     6181 tccggtctgg attacgccaa cttcccgaaa atcaccctca ttgaaaacaa aatgaaggtc
     6241 gatgaaatgg tcaccgtgcg cgatatcact ctgaccagta cctgtgaaca ccattttgtt
     6301 accatcgatg gcaaagcgac ggtggcttat atcccgaaag attcggtgat cggtctgtca
     6361 aaaattaacc gtatcgtgca gttctttgcc cagcgtccgc aggtgcagga acgtctgacg
     6421 cagcaaattc ttattgcgct acaaacgctg ctgggcacca ataacgtggc tgtctcgatc
     6481 gacgcggtgc attactgcgt gaaggcgcgt ggcatccgcg atgcaaccag tgccacaaca
     6541 acgacctctc ttggtggatt gttcaaatcc agtcagaata cgcgccacga gtttctgcgc
     6601 gctgtgcgtc atcacaactg attaaaaggc aggaaccatg gagcgcaacg tcacgctcga
     6661 ttttgttcgc ggcgtcgcca ttctggggat cctgctatta aacatcagcg cctttgggct
     6721 accaaaggcg gcttatctca atcccgcctg gtacggtgct attacgccgc aggatgcatg
     6781 gacctgggca tttcttgatc tcatcggcca ggtgaaattc ctcacgcttt ttgcgctgct
     6841 gtttggtgct ggactgcaaa tgttgctgcc ccgtggcaga cgctggatcc agtcgcggtt
     6901 aacgctgtta gtcttattgg gctttattca cggtttattg ttctgggacg gcgatattct
     6961 gctggcttac gggctggtgg gcttaatctg ctggcggctg gtgcgcgatg cgccatcggt
     7021 aaaaagcctg tttaataccg gcgtcatgct ttatctggtg gggcttggcg ttttgctgtt
     7081 attggggctg atttccgaca gccagaccag ccgcgcctgg acgccggatg catcggctat
     7141 tttgtatgaa aaatactgga agcttcacgg cggcgttgat gcgatcagta atcgtgccga
     7201 tggtgttggc aacagtttac tggcactggg cgcacagtat ggctggcaac tggctgggat
     7261 gatgctcatt ggtgcggcgc tgatgcgcag cggctggctg aaagggcagt tcagcttacg
     7321 tcactatcgt cgtactggtt ttgtgctggt ggcgattggg gtgaccatta accttcctgc
     7381 catcgccctg caatggcagc tggactgggc gtatcgctgg tgtgccttct tacttcaaat
     7441 gccgcgggaa ctgagtgcgc cgtttcaggc gattggctat gcgtcgctgt tttatggctt
     7501 ctggccgcaa ttaagccgct ttaagctggt gctggcaatc gcctgcgtcg gacggatggc
     7561 gctgaccaac tatctattgc aaacgctgat ttgtaccacg cttttttacc acctcggttt
     7621 gtttatgcat tttgatcgcc tggagctgct ggcgtttgtt attccggtat ggctggcgaa
     7681 tatcctcttc tctgttatct ggctgcgtta cttccgccag gggccggtgg aatggctctg
     7741 gcgtcagtta actttgcgtg ctgccggaac ggcaatatct aaaacatcaa gataacgatc
     7801 tggatcacat tcgttaacaa aacggctgta accgtttcca ttgctgtgac tcgattcacg
     7861 aagtcctgta ttcagtgctg acaaaatagc cgccagcaag cagtcattta ctgcaatctc
     7921 ataacaggta gtgaatatga tcaccattcg tgatgtagcg cgtcaggctg gcgtctctgt
     7981 ggcaacggtt tcccgggtgc tcaataacag cacgctggtc agtgccgaca cgcgtgaagc
     8041 agtaatgaaa gccgtgagtg agctggatta tcggccaaat gccaatgccc aggcgctggc
     8101 aactcaggtt agcgatacca ttggcgtggt ggtgatggac gtttctgatg cttttttcgg
     8161 cgcgctggta aaagcggtgg atctggtcgc ccagcagcat cagaaatacg tgctaatcgg
     8221 caatagctat catgaagcgg aaaaagagcg tcacgccatt gaggtgttaa ttcgccagcg
     8281 ttgtaatgcg ttgattgttc actcaaaagc attgagtgac gatgaactgg cgcaatttat
     8341 ggataacatt cccggtatgg tgttaatcaa ccgcgttgtg ccggggtacg cccatcgttg
     8401 cgtttgcctg gataatctca gcggtgcccg aatggcgacg cgcatgttgc tgaataacgg
     8461 tcatcaacgt attggttatc tttcttccag ccacggcatt gaagatgacg ccatgcgtaa
     8521 agcaggctgg atgagtgcgt tgaaagagca ggatattatt ccgccggaaa gctggattgg
     8581 cactggtacg ccggacatgc cgggcggtga ggcggcgatg gttgaactgc tggggcgcaa
     8641 tctacaactt accgctgtat ttgcttataa cgacaatatg gccgctggcg cactgacagc
     8701 attaaaagat aatggcattg cgattccgtt acatctctca atcatcggtt tcgatgatat
     8761 tcccatcgcc cgttacaccg acccgcaatt aacgaccgtg cgttatccca ttgcttcaat
     8821 gactaaatta gccaccgaac tggccttgca gggggcagca ggcaatattg atcctcgtgc
     8881 cagccactgt tttatgccga cgttagtgcg tcgtcattct gtcgcaacgc gccagaatgc
     8941 ggcggcgatc actaactcaa caaatcaggc gatgtaaccg ctttcaatct gtgagtgatt
     9001 tcacagtatc ttaacaatgt gatagctatg attgcaccgt tttaacgttg taacccgtat
     9061 gtaacagtga ataatcactt tcaccgaggt aatagcgtca taacaacaat taaagccgtt
     9121 ttctggagcg ttaccgggca tggaagaacg aattttaaaa agtgagcttc ggcgttcagt
     9181 aacacttcat taactctact gccccgccga gcatttatct caagcactac cctgcataag
     9241 aaaaaccgga gataccatga ataagaaggt gttaaccctg tctgctgtga tggccagcat
     9301 gttattcggt gccgctgcac acgctgctga tactcgcatt ggtgtaacaa tctataagta
     9361 cgacgataac tttatgtctg tagtgcgcaa agctattgag caagatgcga aagccgcgcc
     9421 agatgttcag ctgctgatga atgattctca gaatgaccag tccaagcaga acgatcagat
     9481 cgacgtattg ctggcgaaag gggtgaaggc actggcaatc aacctggttg acccggcagc
     9541 tgcgggtacg gtgattgaga aagcgcgtgg gcaaaacgtg ccggtggttt tcttcaacaa
     9601 agaaccgtct cgtaaggcgc tggatagcta cgacaaagcc tactacgttg gcactgattc
     9661 caaagagtcc ggcattattc agggcgattt gattgctaaa cactgggcgg cgaatcaggg
     9721 ttgggatctg aacaaagacg gtcagattca gttcgtacta ctgaaaggtg agccgggcca
     9781 tccggatgca gaagcacgta ccacttacgt gattaaagag ctgaacgata aaggcatcaa
     9841 aactgaacag ttacagttag ataccgcaat gtgggacacc gctcaggcga aagataagat
     9901 ggacgcctgg ctgtctggcc cgaacgccaa caaaatcgaa gtggttattg ctaacaacga
     9961 tgcgatggca atgggtgcgg ttgaagcgct gaaagcacac aacaagtcca gcattccggt
    10021 gtttggcgtc gatgcgctgc cagaagctct ggcgctggtg aaatccggtg cactggcggg
    10081 caccgtactg aacgatgcta acaaccaggc gaaagcgacc tttgatctgg cgaaaaacct
    10141 ggccgatggc aaaggtgcgg ctgatggcac caactggaaa atcgacaaca aagtggtccg
    10201 cgtaccttat gttggcgtag ataaagacaa cctggctgaa ttcagcaaga aataagactg
    10261 attgggcgca gtcgtttact gcgcccttat tgtatggcca acaaggtaaa attatatggt
    10321 cagctcaacg actccgtctt ccggggaata cttgttggaa atgagcggta tcaacaagtc
    10381 ctttcctggt gttaaggcac ttgataacgt taatttaaaa gtccggccac attctatcca
    10441 tgcattaatg ggggaaaacg gtgcaggaaa atcgacatta ttaaaatgcc tgtttggtat
    10501 ttatcaaaaa gactccggca ccattttatt ccagggtaaa gagatcgatt tccattccgc
    10561 caaagaggca ctggaaaatg gtatttcgat ggtacaccag gagttaaacc tggtattaca
    10621 acgttcggtg atggataaca tgtggctggg gcgatatccc accaaaggca tgtttgtcga
    10681 tcaggacaaa atgtaccgcg aaaccaaagc gatttttgat gaactggata ttgatatcga
    10741 tccgcgtgcg cgcgtcggca cattatccgt ttcgcaaatg cagatgatcg aaatcgccaa
    10801 agcgttttcc tataacgcga aaattgtgat tatggatgaa ccgacttctt cgttaaccga
    10861 aaaagaggtc aatcatctgt tcactattat tcgtaaatta aaagagcgcg gctgcggtat
    10921 tgtttatatc tcgcataaaa tggaagaaat cttccagtta tgtgatgaag ttaccgtatt
    10981 gcgcgacggt cagtggatcg ccaccgaacc gctggcagga ctgacgatgg acaagatcat
    11041 cgccatgatg gttgggcgtt ctcttaatca gcgttttcct gacaaagaaa acaagccggg
    11101 cgaagtcatc ctcgaggtgc gtaacctaac gtcactgcgc cagccgtcga ttcgcgatgt
    11161 ctcgtttgat ctgcataaag gggagatcct cggtattgcc gggctggtgg gggcgaaacg
    11221 taccgatatt gttgagacgt tatttggtat tcgcgagaaa tcggctggca ccattacgtt
    11281 gcacggcaaa cagatcaata accataatgc caacgaagcc ataaaccacg gatttgcact
    11341 ggtaactgag gagcgccgct caacgggaat ttatgcctat ctggatattg gttttaactc
    11401 gttaatttcc aatattcgca actacaaaaa taaagttggt ttactggata actcgcggat
    11461 gaaaagcgat acccagtggg tgattgattc gatgcgggta aaaacgccgg gtcatcggac
    11521 gcaaattggt tcgctctccg gtggtaatca gcaaaaggtg attattggtc gctggctact
    11581 aacgcaacca gaaatattaa tgctcgatga accgacgcgc ggtattgatg tcggggcgaa
    11641 gtttgaaatt tatcagttaa ttgccgaact ggcgaagaaa ggcaagggga ttattattat
    11701 ctcctctgaa atgcctgagt tgttagggat aacagaccgt attctggtca tgagcaatgg
    11761 tctcgtttcc ggaattgtcg atacaaaaac aacaacgcaa aacgaaattc tgcgtcttgc
    11821 gtctttgcac ctttaagatt aggggcttcc catgagtgcg ttaaataaga aaagttttct
    11881 tacttacctg aaagagggcg gtatttacgt cgttctttta gttttgctgg cgattattat
    11941 tttccaggac ccaacatttt taagtctgtt gaacttaagt aatattctca cccagtcatc
    12001 ggtgcgtatt attatcgcgc tcggtgtggc agggttaatt gtcacccagg ggaccgatct
    12061 ttctgctggt cgtcaggtag ggctggcggc agtggtggct gcgacattat tgcagtccat
    12121 ggataacgcc aacaaagtgt tcccggaaat ggcgacgatg ccgattgcgc tggttattct
    12181 gattgtctgt gccattggtg cggtgatcgg tttgatcaac ggtctgatta tcgcttatct
    12241 caacgtgacg ccgttcatta ccacgctcgg cacgatgatc atcgtctatg gaatcaactc
    12301 gctctattac gactttgtcg gggcgtcgcc aatttctggt tttgacagtg gcttctctac
    12361 ctttgctcag ggctttgtcg cgctggggag tttccgtctc tcttacatca ccttctacgc
    12421 gttgattgcg gtggcgttcg tctgggtgtt gtggaacaaa acccgcttcg gtaagaacat
    12481 ttttgccatt ggcggtaacc cggaagcggc aaaagtatct ggtgtcaacg tcggcctgaa
    12541 cctgctgatg atctacgcgt tgtctggcgt gttctatgcc tttggcggga tgttagaagc
    12601 cggacgtatc ggctctgcca ccaacaacct cggctttatg tatgagctgg atgctatcgc
    12661 ggcgtgcgtg gtaggcggtg tatcgttcag cggcggtgtg gggacggtga ttggcgtggt
    12721 gaccggggta attattttta ccgtcatcaa ctatggcctg acgtatatcg gcgtaaaccc
    12781 atactggcag tacatcatca aaggggcgat tattatcttc gccgtagcgc tggattcact
    12841 gaaatacgcg cgtaagaagt gatgtgattg tggatattgc cggatgcgct gcatccggca
    12901 atcggtgcca aatgccggat gcgtctctgg ttttacaacg ttaccgggtg ttctttctcg
    12961 cctttcttaa acttcacttc cccgagctca atacaaccca ccgggcagac gtgaccacaa
    13021 agcagacaac ccacacattt ctcggtatta caatgcggtg tgcgggtttt ctcgctccat
    13081 tccatcgcct ggtgaccgcc gtcgtaacag gaaatataac agcgtccaca gccaacacat
    13141 ttatcaagat tgatacgggg atagacaata taactgcggt ctaaatcttc cgccgggaca
    13201 atattgttat tcgccagacc taccatctcc tgcagcgaat caaatccctg atcggcgaga
    13261 taatgcgaca acccgcttgc catatcttcc actatccgat acccgtactg catgatgccg
    13321 gtggtcacct gtaacgttgc tgcgccgagc aataaaaact cagccgcatc ctcccaggtt
    13381 tcaatgccgc cgataccgct gattgggaaa tcgcgcagtt ctggatgggt gcgcatctgc
    13441 tgaatgaagc gcagggcgat cggttttacc gctttaccgg aatatccgga aatactcgat
    13501 tttccgttaa cgatcggcat accgattttc tgattaagat cgatattggt gatggattta
    13561 acggtgttaa tcgccgcaat gccatctgcg ccgccgcgct tcgccgccag cgccacttcg
    13621 cacatatcac cgatattcgg cgtcatcttc gccagcattg gcagcgtgga accccgtttc
    13681 actgcccgac aatatttttc taccagctcc gggctttgcc cgacatcgct acccatcgca
    13741 tgagaagtca tttgcggaca ggagaagtta cactcgatca tatccgcgcc agcttcttgc
    13801 accaggcgcg ccagctcctc ccattgctgc tcattttccc ccatgatcga agcgatcaat
    13861 actttgtccg ggtaatcttc cttcagctga cgcagggcgg ccagattctc ttccaacgga
    13921 tgttcagcaa tctgctccat atttttgaag ccgataaaac cggtatcttc tttcaccaga
    13981 tgatcaaaac gcggcgagac ttcgttggcg ataaaaaagc cgatcgtttt aaacaccacg
    14041 ccgccccaac ctgtgtcgta ggctttggca cacatctcat agcagttgcc tacaggcgaa
    14101 gaagagaggc agaacgggtt gggaaacttc acgccgcaaa aagtaatcga aagatctttc
    14161 gttaacatga gcaagctccc tctaaatagt gatgaatcgc ctcggcggct tctttcccgg
    14221 ttttcacggc atagaccacg gttttgtcac cctcaacaat atcgccagca gcaaagactt
    14281 gcggatcgcg ggtctgataa ttttgtgttt tgatggtgtt acgctgcggc tctaactccg
    14341 caaaggcatc cagtctggca tgctgaccga cggcgagaat aattttatct gccgccatcg
    14401 tcagttcgcc cggtaaccgt acatgcttaa acgtgacttt attgccttcc acggctactg
    14461 gcgtgaatcc atcgatgatc gaaacgccca gctcccgggc gctggcaaac tctttttcgc
    14521 ttgccggaaa ctcatctaac tcttcacgcg ctacgcaggt taccgcctga cagccgagaa
    14581 ctttcagcgt gctggctacg tccatcgcaa catcaccgcc gccgataatt aatgcgcttt
    14641 gtggaatgct gatatcgcct tgcgcctgtc gtgcacattg caggaagtcg acggcaatct
    14701 caacgtcgct atgctcaaac agcgataacc cggaaccgct cgataacccc acggtgacca
    14761 gtaccgcgcg gttttctgct ttcagctgct caagggtgag tgtgttaccg acttcgttgt
    14821 tgcacttaat ggtcacgccc attttttcaa tacgggcgat ctctgcatcc agcactgatt
    14881 gcggtaaccg gaattgcgga ataccgttac gcaaccagcc accggggtgc gcttctttct
    14941 cataaatcgt gacgtcataa ccctggtttg tcagtgttac actggcctgc aatccggcag
    15001 gacctgcgcc aataatcgcg actttgccga gcgttttagt accgggctga taaatttcca
    15061 tcccggtttg ttgttcaaaa tcagtaacaa aacgctgtaa gcgaccgata tcaatgggag
    15121 tatcaacacc ggcacgggta caaccgcttt gacataattt ctccgtcggg cacactctgg
    15181 cacaaacggc accgagggca ttattttcgc gaattgtctc ggcagcgcct ttaaaattac
    15241 gaaagtagat tgagcgaata aatttccccg gatcggtctg tgccgggcaa gcctgactac
    15301 agggagcgtc atgacataat aaacagcgag aggcttcttt aatcgccagt aaaggcgtaa
    15361 aagccggagt gagttcatcc agataatttt gttgcggcat gtgatcttcc ttaatgctat
    15421 gagtaattaa ggcgaacgct aaatatactc gtcatacttc aagttgcatg tgctgcgtct
    15481 gcgttcgctc accccagtca cttacttatg taagctcctg gggactcact cacttgtcgc
    15541 cttcctgcaa ctcgaattat ttagagtata aatattgcgt gctgatatta aggctatgcg
    15601 cgtggggaaa tttatactgt gaaaggattc acaaatagct gcggttcaga atgttatatc
    15661 cgcagcttat tgagtgaagt aataattatt ctcaataatt aacagaaaag agaaaatatt
    15721 aacgatagaa aataagataa acaatcatgc cgatagataa aattccgaag ccgatatatg
    15781 ccaccatata agcgaaaaag agtccgggat tttttgctcg ccagacatac accggttccg
    15841 gtgcattttt aacccgttta tcaatcgcgc ctgcgcgcca gcctttccat gcttcgcgga
    15901 aacaaaatat cggaataagg aaaaaaacgg caacgacaaa aaactgctgt acatccataa
    15961 ctactttcct tgtttctttt gtaattcaag taactgttcg ggtgtgacgg cgggataccc
    16021 ctgcgcatct tccggcacct ggtgcatagc cggaataggg accagcggcc ctaaaaaacg
    16081 cggttcacgt ttaaaaatat aaaggtcagc cagcgcaccg aaacgggcgg caaattcgcg
    16141 aatacgtact gacagcatat ctttcggtga cggtacggca taacactgag cctgaatccc
    16201 catatgcagc gcaataaata atgcccgctc acagtggaaa cgttgggtga taataatgaa
    16261 atcattagta tcgaaaactt tgcgtgtacg cacgatggaa tccagcgtac gaaagcctgc
    16321 gtaatcgaga acaatatctg atgggtcgac accagcagcg attaaatctt tgcgcatggt
    16381 catcggctca ttataacttt gcaatgcgtt atcgccgctc agtaatagat aatttacctt
    16441 accgctgtta taggcattaa tcgctccttg aatgcggtag cgataatact gattaattac
    16501 gccagtacga taatattttg ctgttccgag caccacaccg acctggcggt aggggagatc
    16561 ctgcaattcg tcgtagatat aaggcgcggt tttccagctc atccagcgat cgaggccgag
    16621 cacagtcaac agcagcaagc cgatcaggac taacaggctg aggaacacgc gctttaacat
    16681 gcaggagact cgatctaagg aaaatttcca tcaggctact ttacccgctg gttttgcgca
    16741 agaaacggca aactgtttgc cgggaatttc gacaccagga gagggctggc aagccggaca
    16801 aggcgttcac gccgcatccg gcaccaggct taagcgagaa gcactcggtc gatactgtga
    16861 cagccgagag ctttcagcgt tgcggaggtg gcatcccact gaatcaacgg cgcatcggct
    16921 ttttctgcca gaaccgcgcg ctggatatcc gggtaatcat aacccttgag attcaacaga
    16981 attaacgctc cctgcaacgg tggcagagtc gggttgaatg cggcgttttc agcgtagctg
    17041 ccactgaaaa tacgaccgtc tttacattcc agcgcgacac cgcttggcga cttactgtaa
    17101 ggcatgtgcg aacggtttgc cgctgcaatc gccgcctgag aaagcgcatc accagtcagc
    17161 gcatagccgt gatcctgctc gtccatcagc agcgttttaa tctccagatc tttcggccca
    17221 aaggcatccg gcagatagtc acgcagcgcg tgtgcctcgc ggcccggcag atgaatacgc
    17281 agatccagac cgctgttcag ttcattcata aactgacggc agtgaccaca aggcgtgtag
    17341 ttaacggtga tggctgcaag cgctttttca ccactcaacc aggcgtggct gatcgcgctt
    17401 tgttcggcat gaacggtttg ctgcattgtc gcaccaataa attccatatt ggcaccgaaa
    17461 taccaggttc cgctcacacc gcgcgcaatt gcgccaacat taaaattcga caatggcgta
    17521 cgcgcacagg ccgccgccag cggaagtagt gcgaatgcca gcgcgtcttc gtccagcccc
    17581 gttgcgctct tcagcgatga gacttgctcc ccggtcaaca aagcggggaa gtacttgtct
    17641 gccagaatag gttccagtgc agattgcaag ttatccgcaa gttgggcaaa agcggtttga
    17701 aaacgtggat gcatggcgtt gcctcataat catgtaatgg gataacagtt tacgaacccg
    17761 ttatggcttt atatgtgatc tgaatctcat taattatgca acgtgtgaaa cttacatcaa
    17821 aaatgcgcga cgcatcgcaa attttagccc attactgcca gaataatcgg gaaaaggaac
    17881 ggtgcgatca gcgaagtaat tatcccgcat aacaccagcg ctagcgaact aaatgcacct
    17941 tcctgataat ccagctcggc gcagcgcgcc gtaccgaggg cgtgcgaggc agtccccatt
    18001 gccagaccgc gcgcagcttt ggtacgaata cgcatcgcat taagcaatgt atggccaaat
    18061 accgcgccga ggatgccgac gaaaatcacg caaactgcgc tgattgccgg aataccgcca
    18121 atactgccgc caaccgccat tgcaataggc gtggtgactg atttcggcag gattgacgcg
    18181 gcgatttccg gtgaagcgcc catcaacaat gccacggaag tccccgtcac catcgcaacc
    18241 acgctgccga taaaacagat ggtgatgatc gatttccagc gcgcgcggat ctggtgtagc
    18301 tgctcatata aaggataggc cagcgcgacg accgccggtt gcagcagatc gtttagcact
    18361 tcgctacctt taaagtagct gtcgtaagag atgccggtca gcattaaaaa aggaatgatc
    18421 accaccattg ctaccagcaa cgggttaagc aggggaaact tataccgtgc cgccagtttg
    18481 cgggcggcaa aaaagacaat caacgtcagc ggtaatgacc accagatatt cgccatcatt
    18541 cttctgatcc tttctgacct acgactttac gttcaccgtg taccagttgc gaactccagc
    18601 tcaccaccag aaaaaccacc agcgtactga ttgcacagga aaccactacc gggccaaact
    18661 gtgcgcggag caaatcaaaa tattgcatga cgcctacgcc aatcggcaca aacaatagcg
    18721 ccatatagcg aatcagtacg tagcaccccg gattgaccca tttcgccgga agaatttgca
    18781 aggccagcag gacaaacagg atcagcatcc cgatgatgct gcccggaatg gttaccggta
    18841 gcagggaagc aatgaaaatg cctgcataca ggcaggcata aatcaggacg aaagcgcgta
    18901 aatattgcca gataatgttc agtgtcttac tcataatcaa caccccagat gagatggatt
    18961 catcatacaa ttaattcgta aaacgtgcta ccgatcacat taagaaatca gcgcttattt
    19021 tttcgctccc cggtattacg caatgagcag cgaactcagg catacatcaa gcatagagag
    19081 ggattgcagt ttgcagcaat ccctctttgc agacctttaa tccgttgacc ataggcgcta
    19141 acatagcgct tcattttttt gcgggtgatg atatgcgtgt gttactggca ccgatggagg
    19201 gagtgcttga ctctctggtg cgtgaattgc tgaccgaagt taacgactac gatctgtgca
    19261 tcaccgagtt tgtccgcgtg gtggatcaac tgctgccggt aaaagtcttt catcgcattt
    19321 gccctgagct acaaaacgcc agccggacac catctggtac gctggtgcgc gtgcagttgt
    19381 taggtcagtt cccacaatgg ctggcagaga acgccgcccg tgcggtggag ttaggttcct
    19441 ggggcgtgga tctcaattgc ggctgcccgt cgaaaacggt caacggtagc ggtggtgggg
    19501 cgacgttact caaagatcct gaactcatct accagggtgc aaaagcgatg cgtgaagctg
    19561 taccggcgca tttgcccgtc agcgtgaaag tgcgtctggg ctgggacagc ggtgagaaga
    19621 aatttgaaat cgccgatgcg gttcaacagg ctggtgctac ggagctggtg gtgcatggac
    19681 ggacaaaaga gcagggttac cgcgcggagc atattgactg gcaggcgatt ggcgagattc
    19741 gccagcggct gaatattccg gtgattgcca acggtgaaat ctgggactgg cagagtgcgc
    19801 aacaatgtat ggcgatcagc ggctgcgacg cagtgatgat tggtcgcagg gcgctcaata
    19861 ttcccaacct gagccgggtg gtaaaatata acgaaccgcg aatgccgtgg ccggaggtgg
    19921 ttgctttgct gcaaaaatat acccgtctgg aaaagcaggg cgataccggg ttatatcacg
    19981 ttgcgcggat taaacagtgg ttgagttatt tgcgtaaaga atacgatgaa gcaacggaat
    20041 tatttcagca tgttcgggtg ttgaataatt cccctgatat tgcaagggct attcaggcaa
    20101 ttgatatcga gaaactctaa aaaaccatag tgatgttttt tgacaatgtg ttagctcttt
    20161 atcaataggc cgcacggttg tttggtggcg tcaaaat
//
LOCUS       DAZGVQ010000093        19343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000093 DAZGVQ010000000
VERSION     DAZGVQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.094"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            339..3854
                     /locus_tag="ACHLAG_003207"
     CDS             339..3854
                     /locus_tag="ACHLAG_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFP0656384.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASF
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLNNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMARIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLEEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREKSNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAAWLAENQQNIEAHYGKSLHEVIGIVTPFSAQVSTIKQALGKQGISTGANEKSL
                     TVGTVHSLQGAERAIVVFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPPSSPRGLLAKYLFESEKNALSFDYKERKDLKTSGTKIYTLHGVEQHDKFLNQTF
                     ENTSKYITIVSPWLTWQKLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHKDYEKRKE
                     KQQNLKGTLEKLNAFGITTKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLEKRQV"
     gene            complement(3963..5462)
                     /locus_tag="ACHLAG_003208"
     CDS             complement(3963..5462)
                     /locus_tag="ACHLAG_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP0656385.1"
                     /translation="MNIRKLFCPGNTPRILLFLFFFVVSAITSIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTADEAAEF
                     TGMIPVYHFLVSAAILVFIVIFWRAHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     ATDTLREMQRYKQLSQHGADNWKILPGVPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSTAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKSSPKVIFL
                     HMIGSHPNPCDRLNSWPNHYLEQYPRKIACYLASISKLDNFLGQLDGILRRHSRHFAM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLINTASDITSHQSVSRKISARHFTGIFQ
                     WLTGIRTENIPPFNPLTDEDNEPVMVFNGEKNVPADSLKPQPLILPDRR"
     gene            complement(5618..6358)
                     /locus_tag="ACHLAG_003209"
     CDS             complement(5618..6358)
                     /locus_tag="ACHLAG_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFP0656386.1"
                     /translation="MKKVIVVSVFAMAGMFSAQALADESKTGFYMTGKAGTSVLSLSN
                     QRLIDGEGEWADKYKGGNDHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NVDKDSWSGGYWRDDLKNEVSVNTLMLNTYYDFRNDSAFTPWISAGIGYARVHQKTTG
                     ISIWDHGYGNSGRESLSRSGSADNFAWSLGAGVRYDVTPDIALDLSYRYLDAGDASVS
                     YKDEWGDKYKSEVDVKSHDIMLGMTYNF"
     gene            6784..8610
                     /gene="atoS"
                     /locus_tag="ACHLAG_003210"
     CDS             6784..8610
                     /gene="atoS"
                     /locus_tag="ACHLAG_003210"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFP0656387.1"
                     /translation="MNYIKWIYPRRLRNQMILMAILMVIVPTLAIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRQELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDLIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARIDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            8607..9992
                     /gene="atoC"
                     /locus_tag="ACHLAG_003211"
     CDS             8607..9992
                     /gene="atoC"
                     /locus_tag="ACHLAG_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFP0656388.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRPPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTSPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            10188..10850
                     /gene="atoD"
                     /locus_tag="ACHLAG_003212"
     CDS             10188..10850
                     /gene="atoD"
                     /locus_tag="ACHLAG_003212"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFP0656389.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            10850..11500
                     /gene="atoA"
                     /locus_tag="ACHLAG_003213"
     CDS             10850..11500
                     /gene="atoA"
                     /locus_tag="ACHLAG_003213"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFP0656390.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGARKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            11497..12819
                     /gene="atoE"
                     /locus_tag="ACHLAG_003214"
     CDS             11497..12819
                     /gene="atoE"
                     /locus_tag="ACHLAG_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFP0656391.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLQKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            12850..14034
                     /gene="atoB"
                     /locus_tag="ACHLAG_003215"
     CDS             12850..14034
                     /gene="atoB"
                     /locus_tag="ACHLAG_003215"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFP0656392.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKADSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMDESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(14444..14629)
                     /locus_tag="ACHLAG_003216"
                     /pseudo
     CDS             complement(14444..14629)
                     /locus_tag="ACHLAG_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063945310.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            14690..15823
                     /locus_tag="ACHLAG_003217"
     CDS             14690..15823
                     /locus_tag="ACHLAG_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc45 family transposase"
                     /protein_id="HFP0656393.1"
                     /translation="MHSENIAAYIGLDVHKETLAVAIAAPERLGEVRYYGTINNEAQA
                     VRRLFQKLQGLYGNILSCYEAGPCGFGLYHQLTAMNIKCQVIAPSRIPKSPTDRIKND
                     HRDAISLARLLRAGELTPVWIPDLTHEAMRDLIRARAAAKRDSRVARQRILSMLLRTD
                     KRYAGKHWTGKHRTWLANQSFSQPSQQIAFQHYCQSLEQIEDRILQLDQEISRLLPEW
                     SLCNLVCQLQALKGVGQLTAITLVAELGDFSRFSSPKQLMAFLGLVPGEYSSGNSIRP
                     RGITKVGNSELRRLLYEAAWSYRTPAKVGAWLIYYRPDSVTQYSKDIAWKAQQRLCSR
                     YRSLTAKGKKSQVAITAVARELTGFMWDIALAAQSSFSQQKQN"
     gene            complement(16076..16546)
                     /locus_tag="ACHLAG_003218"
                     /pseudo
     CDS             complement(16076..16546)
                     /locus_tag="ACHLAG_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            16814..17053
                     /locus_tag="ACHLAG_003219"
                     /pseudo
     CDS             16814..17053
                     /locus_tag="ACHLAG_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088287.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            17351..17539
                     /locus_tag="ACHLAG_003220"
     CDS             17351..17539
                     /locus_tag="ACHLAG_003220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656394.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTKPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            17867..18061
                     /locus_tag="ACHLAG_003221"
     CDS             17867..18061
                     /locus_tag="ACHLAG_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656395.1"
                     /translation="MNENRIKRLEQLLQARQREFATKWGRAALPHERLIYFPLEDLIK
                     NNGKKIRPDKKHFLECNEDK"
     gene            complement(18860..19184)
                     /locus_tag="ACHLAG_003222"
                     /pseudo
     CDS             complement(18860..19184)
                     /locus_tag="ACHLAG_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5274 a         4601 c         4609 g         4859 t
ORIGIN      
        1 tactttatcg tcaaacacgt tatagctggc atcaaaaacc acagatttac cctgccagcg
       61 ggtaatcccc tcctctggcg ttttcggttg aactggtatc cccgtccaac aaaggcagcc
      121 attataaaca gtaaaaaatt gtattcgctg ttatgaaatc aatagtcctg acgtactgtt
      181 gaatgtgctt gtgttctgaa cctcgtacaa ccagtacatt tgatatcgaa ttataaacaa
      241 aacattgcat ccaacttttt caatatggac aatagttgtc catacggtgg ttttatattc
      301 gataaatcaa tggatttcat tgagtcacag gactggtaat ggatgaaaat gctttagggt
      361 ttgcctcata ctggcgcaac tcgcttgcgg atgctgagtc aggaaagggc agttttgaac
      421 ggaaagacgc caaaaatttc actcactggc atgggatagc ggcgggacgt cttgacgaag
      481 cgattgtcag taaatttttt aagggagaaa aagacgatgt agaaacggtc gatgtcatct
      541 tgcgcccaaa agtttatttt cggttactgc agcatggtaa ggaccgttct gcaggtgcgc
      601 ctgatattgt taccccgata gtgacgccag ccttgctaag ccgtgagggt tttttatatc
      661 cgacgccagc gacctccatt cccagagacc tgcttgaacc tttgccaaaa ggagcatttt
      721 cgattggtga gattgggcag tatgacaaat acaagacgac ccataccacg ttctctatca
      781 actttgatga cagcgttgat aagactgccg aaacggatga agaacgggaa gcacgatatg
      841 ccgccttgca gcaggagtgg cgtcaatatc tgtatgactc agagaggcta ctgaagagcg
      901 ttgccggcga ctggattgaa aaacctgagc aatatgaact cgctgagcac ggttatattg
      961 ttaaaacggc tcaatctggc ggtgccagtt tccatatcct ttctctttat gatcacctgc
     1021 ttgtttgcaa taaggatgtg ccgctcttca atcgattcgc ctcgcgagag gttcatgctg
     1081 cagagtcttt gctggcccct ggagcaaaat tcagcgacag gcttggacac tccggagata
     1141 agtttccgct ggcaaaggct cagcgcgatg ccttaagcca ttttctggat gccagacatg
     1201 gcgatatcct tgctgttaat ggccctccgg gaaccggaaa aaccacgctg gtgctttcta
     1261 tcatcgccac gcagtgggcc agagcggctc tcgaaaaatc tgagcctccg gttattatcg
     1321 cgacttcaac gaataaccag gctgtaacga acattattga ggcattcggg aaagactttt
     1381 cgcaaggttc aggtgcgatg gccgggcgat ggttgccaga gctgaaaagc ttcggcgctt
     1441 attttccctc aagcagtcgt aaagctgagg cagccaaaaa atatcaaact gaagatttct
     1501 tcaaccaggt tgagtcaaaa gagtatgtag aggatgcact gctgttttat ctggaaaagg
     1561 ctaaggcagc ctttcctgga aaagagtgtt catcccctga aaaggtcatt gaactcctgc
     1621 atggtcagtt ggcagcaaaa tctgagcaac tgataagact gaacgcaaca tggcaaacgt
     1681 taagccagat tcgggctgcg cgtgagctta ttgctaatga tattgagcaa tatctcaata
     1741 atttaaataa attactttcc ggacaagaac aaaaagtcac tctactgaag agtgctaaaa
     1801 cggaatggaa aaaatatcgc gccggtgaat cactgatcta ttcattattt tcctggctcc
     1861 cggcggttcg caataagcga cagtaccaaa tacagctgtt tctcgaagat aaattaggcg
     1921 cgctgattgc aggaaatcag tggtctgatc ctgaaactat cgaacgtaat attgatgggc
     1981 tgctcaattc cgctgagcgc gagcaaacaa cataccggca gcagattgac tccgcccatg
     2041 aaatcgttct taaagaacag caggcggttc aggagtggca gaggctggca tttgatttag
     2101 ggtatgaggg cgacgaggaa ctgagcttct cacaggccga tgaactggct gatacgcaga
     2161 ttcgcttccc tgcattttta ctgacgactc actactggga aggtcgttgg ctgatggata
     2221 tggccagaat tgatgatctg caggacgaga agaagaaaaa aggtgctaaa ggggtaaccg
     2281 cccgttggca acgtcgaatg aaactcacgc catgtgtggt gatgacatgc tatatgctgc
     2341 ccggcaatat gcagataagt gagcacaaag gacaacgtaa attcgagaaa agttatttgt
     2401 atgattttgc cgatttactc attgtcgatg aagccgggca ggtgcttcct gaagtggctg
     2461 ctgcctcgtt tgcattagct aagaaggcat tagtgattgg cgatacggag cagatcccgc
     2521 caatatggag tattgctccc gcgattgatg tcggtaacat gctggaggaa aaaattctgt
     2581 cgggcagtac gcaagaagag attaccgaga aatatacggc aatcgcagac cttggtaaaa
     2641 gtgccgcatc tggcagcgtt atgaaaatag cgcagtttgc ttcgcgctat caatatgatc
     2701 ccgaactggc tcgtggtatg tacctatatg aacaccgccg gtgctacgac aatattattg
     2761 gatactgtaa tacgctctgc tatcacggta agttgttgcc taaaagaggg cgtgaaaaga
     2821 gcaatttaat gcccgcaatg gggtatctcc atattgatgg taaaggagag ctggcaagta
     2881 gtggaagtcg atataatttg cttgaggctg aaacaatagc ggcctggttg gcagagaacc
     2941 agcaaaatat tgaagcgcat tacggcaaat cgcttcatga agttatcggt atcgtgacgc
     3001 cttttagcgc gcaggtatcc actatcaaac aggcgctggg taaacaaggt atcagtactg
     3061 gcgcgaatga aaagtcgctc acagtgggca ccgtgcactc tcttcaggga gcggaaagag
     3121 cgattgtggt attctcgcca gtctattcaa aacatgaaga cggcgggttt attgatagcg
     3181 ataacagcat gctgaatgtt gcagtctccc gtgcgaagga tagcttcctg gtcttcggcg
     3241 atatggatct gtttgaggtc cagccgccct catctccgcg aggattgctg gcaaaatacc
     3301 tctttgagtc agagaagaat gcgctctctt ttgattataa agagcgtaag gatttaaaaa
     3361 cctccgggac caaaatctac acacttcatg gtgtggagca acatgataaa ttcctgaatc
     3421 agacatttga aaataccagt aaatacatca cgatagtttc tccatggctg acctggcaaa
     3481 agctggagca aaccggtttt cttgattcca tgattgcggc gtgttcacgt ggtattaacg
     3541 tcacgatagt cactgacaga agctacaaca ctgaacataa agattatgag aaacgaaaag
     3601 agaagcagca gaatcttaaa gggacgctgg aaaaactgaa tgcttttggt attactacaa
     3661 agctggtaaa tcgcgttcat agcaaaattg ttattggtga tgatggtttg ctgtgcgtgg
     3721 gatcgttcaa ctggtttagc gcaacgcgag aagcgcgata tgaacgatac gatacctcaa
     3781 tggtttattg cggtgataac ctgaagggtg agattgaggc tatttataat agtcttgaga
     3841 agcgtcaggt ttagagcagg aatggtgagt agccatctta tcgatcgttt tcgagcgtaa
     3901 gatggctgaa tggaatagct attattgcac agtccttaat tataacattc ataccaacat
     3961 gattatcttc tgtccggaag aatcaaaggc tgcggtttca gactgtctgc cggcacattt
     4021 ttctctccgt taaaaaccat aacgggttca ttatcttcgt ctgtcagcgg attgaatggc
     4081 ggtatatttt cggtacgaat accggtcagc cactgaaaaa tgcctgtgaa atgacgggca
     4141 ctgatttttc tgctgacgga ctgatgagac gtgatgtcac tggcggtatt aatcagggga
     4201 acgctgtagc ctccctgcac atgaccatca tgatgaacag gattagcact gtcgctgacc
     4261 gacagcccat ggtcagaaaa gtaaagcata gcgaaatgac gggaatgccg gcgaaggata
     4321 ccatcaagct gaccgagaaa gttatccagt ttactgatgc tggcgaggta acaggcaatc
     4381 tttcggggat actgctccag gtaatgattc ggccaggagt taagtcggtc acacggattc
     4441 ggatgagagc caatcatgtg caggaaaatc accttcggag aggatttatc cgccagcgca
     4501 cgttctgttt cctgtaacaa caacatgtca tccgttttac gggaagcaaa gctgcctttc
     4561 ttgaggaaaa cggtatgctc cgcatcagaa gcaataacag agatgcgtgt atcatgctcc
     4621 cccagttttc cctgattgga tatccaccat gtgctgtatc ctgcttttgc tgccagcgcc
     4681 accacgttgt tgccggagtc agggttctgc tcatagtcat aaatcagtgt ccggctcagg
     4741 gaaggtacgg tactggctgc tgtcgatgta tagccgtcaa taaataaacc gggagcagta
     4801 ttcagccacg gcgtggttgg cacgggatag ccatataccg acatataatc cctgcgcaca
     4861 ctctcaccag taacgataac aatcgtgtca tacaacggca cacccggcag gattttccag
     4921 ttgtcagccc cgtgctgact cagttgttta taacgctgca tttcacgcaa tgtgtcagtt
     4981 gcccccacaa cagttccttt aaccatccgc aacggccagc tgtttacaga gcataatacg
     5041 aatagcagca gtgccagcca gttacggtga ccgcggtggt gtgcccgcca gaaaatcact
     5101 atgaatacca gaatcgcggc actgaccaga aaatgataaa caggaatcat cccggtaaac
     5161 tctgcagcct catcagccgt ggtctgcagc aacgcgacaa taaaactgtt gttgatttta
     5221 ccgtacgtca tacccgcagg cgcatacagt gcacaacaga acagaaataa cagcgctgta
     5281 atggatgtga gggtatttct gtgtgcaagg agaagaagga gaaacagaag cagcacattt
     5341 cctgtggcat tcttctcagt gtatccgcat gcaattgagg ttattgcaga aacaacaaaa
     5401 aagaataaaa acaataaaat ccggggtgtg ttgcccggac aaaacaattt cctgatattc
     5461 atcggagtat atcgacaaca ttattatgaa gagaacaaga taataaaaat cagaaattat
     5521 ctgtaaaaca gataacagac aaccctgcag taatagactg cagggtattg ctgtttaatt
     5581 gcagaagata tgcaattatc gttcaggagc agtgtcgtca gaagttataa gtcataccaa
     5641 gcatgatgtc atgactttta acatcaactt ctgacttata tttatcgccc cactcgtcct
     5701 tataactcac actggcatca cctgcatcaa gatagcgata gctgaggtcc agcgcgatat
     5761 ccggggtgac gtcatagcgg acacctgcgc caaggctcca tgcaaagtta tcagcagagc
     5821 ctgagcgtga caacgattcg cgaccactgt ttccgtaccc atgatcccag atactgatac
     5881 cggttgtttt ctggtgaact ctggcgtagc caatccctgc agatatccat ggtgtgaatg
     5941 cgctgtcatt ccggaagtca tagtacgtat tcagcatcag tgtgttgact gacacttcat
     6001 tcttcaggtc atcacgccag taaccacctg accaactgtc tttatctacg ttatacttcg
     6061 aatcagcttt tccacgagcg taaaactcca gttccgtacg aaccggaata ctgaactgcg
     6121 gataaaaatc ataaccagcc gcgataccgc cactgaatac cgtatcatgg tcattaccac
     6181 ctttatattt gtccgcccac tctccttcac catcgataag acgctggttg gaaagagata
     6241 aaacggaagt ccccgcctta ccggtcatat aaaagcctgt tttgctctca tcagccaagg
     6301 cctgggctga aaacataccc gccattgcaa atactgaaac cacaataacc ttttttattt
     6361 caatcatctc cgttccctac ccgacaaaga ggctcaccta ttctaaaacc atgcaaaaga
     6421 cagggtaaaa agagaaattt cgatcgaatc gatcgacaaa atcgatcata catgatgaag
     6481 atttcttatc gaatccataa aaatagtgac aggtaacggg tactgcagga acaatcagaa
     6541 atggaagttt gggaaagtgc catagcacta cgtcgttgct aacatagagg aacagtgctt
     6601 tgttgataag atcctttata cgacgaatct attggatgaa aaataaacta cgtaatcacc
     6661 tgattctcac tgatattgcc aattctggta ggattgagtg attcttcgct tacaaaacga
     6721 tgctcaggaa tatgataatt gttatatatc gatgtataca ggacaatatt agtggatctc
     6781 agcatgaatt atataaagtg gatttaccca cgccgcttac gcaatcaaat gatcctgatg
     6841 gcaatcctga tggtcattgt cccaacgctt gctattggtt atatcgtaga aacggaagga
     6901 cgttcagcag tcttatctga aaaagagaaa aaactttctg ccgtggtcaa cctgcttaat
     6961 caggcgctag gcgatcgcta cgatctctac atcgacttac cgcgtgagga gcgtatccgc
     7021 gcattaaatg cagaacttgc ccccattacc gaaaatatca ctcacgcctt ccctggcatc
     7081 ggcgctggtt attacaacaa aacgctggat gcgataatca cctacgcgcc ttcagcgcta
     7141 tatcagaata atgtgggcgt taccattgcc gcagatcacc ctggtcgcga agtcatgcgt
     7201 acaaataccc ctttggtgta ttcaggcagg caggtgcgcg gcgatatttt gaattcaatg
     7261 atccccattg agcgtaatgg tgaaatcctc ggctatatct gggccaatga attaaccgaa
     7321 gatattcgcc gccaggcctg gaaaatggat gtgaggatta tcattgtgct caccgccggt
     7381 ttgctgataa gcctgctgtt gattgtcctt ttctcccgtc gcctgagcgc caatattgat
     7441 atcatcaccg atggcctctc gactctggca caaaatattc ccactcgatt accacaattg
     7501 cccggtgaaa tggggcaaat cagtcagagt gttaacaacc tcgcccaggc actgcgtgaa
     7561 acgcggacac ttaacgatct gattattgaa aacgctgccg atggcgtcat tgccattgac
     7621 cgtcagggtg atgtaaccac catgaacccg gcagcagaag tcatcactgg ctatcaacgt
     7681 caggaactgg tagggcagcc ttactccatg ttgttcgaca atactcagtt ctacagtcca
     7741 gtactggata cgctggaaca tggcaccgaa catgtagcgc tggagatcag ttttccaggc
     7801 cgtgaccgca ccattgaact cagtgtcact accagtcgaa ttcataacac gcatggtgaa
     7861 atgataggtg ctttggtgat tttctctgat ttaactgccc gcaaagaaac ccagcgccgc
     7921 atggcgcaag cagaacgcct cgccacactg ggtgagctga tggctggcgt cgcgcatgaa
     7981 gtacgtaatc cgctaacggc tattcgtggt tatgtacaga tcttgcgcca acaaaccagc
     8041 gacctaatac atcaggaata tctgtccgta gtactcaaag aaatcgattc aattaacaaa
     8101 gttattcagc aattgctcga attttcacgt ccacgccaca gtcaatggca acaagtcagc
     8161 ctcaatgcat tggttgaaga aactctggta ctggtacaaa ccgccggcgt acaagcgcgg
     8221 atcgacttca taagcgaact ggataatgaa ttaagcccga ttaacgccga tcgtgaactg
     8281 ctcaaacagg tactactgaa tatcctgatc aatgccgtcc aggctatcag tgcacgaggg
     8341 aaaattcgca ttcgaacctg gcagtacagc gactcacaac aggccatttc gatagaggac
     8401 aacggctgtg gcattgatct ctcgctgcaa aaaaagatct tcgatccctt tttcaccacc
     8461 aaagcttcag gaaccgggct tggtctggcg ttaagtcaac gcatcattaa tgcccatcag
     8521 ggtgatattc gcgtcgccag tttgccgggc tacggcgcaa ccttcaccct tattttaccg
     8581 atcaatccgc agggaaatca gactgtatga ctgctattaa tcgcatcctt attgtggatg
     8641 atgaagataa tgttcgccgt atgctgagca ccgcttttgc actacaagga ttcgaaacac
     8701 attgtgcgaa caacggacgc acagcattac acctgtttgc cgatattcac cctgacgtgg
     8761 tgttgatgga tatccgcatg ccagagatgg acggcatcaa ggcactcaag gagatgcgca
     8821 gccatgagac ccggcccccc gttattctga tgacggccta tgctgaagtg gaaaccgccg
     8881 tcgaagcgct acgctgcggg gccttcgact atgttattaa accgtttgat ctcgatgagt
     8941 tgaatttaat cgttcagcgc gctttacaac tccagtcaat gaaaaaagag atccgtcatc
     9001 tgcaccaggc actgagcacc agctggcaat gggggcacat tctcaccaac agcccggcga
     9061 tgatggatat ctgcaaagac accgccaaaa ttgccctttc tcaggccagc gtcttgatta
     9121 gcggtgaaag cggcaccggg aaagagttga ttgccagagc gattcactac aattcgcggc
     9181 gggcaaaggg gccgttcatt aaagtcaact gcgcggcgct gccggaatcg ttgctcgaaa
     9241 gtgaactgtt tggtcatgaa aaaggtgcat ttactggtgc acaaaccttg cgtcagggat
     9301 tatttgaacg tgccaacgaa ggtactctgc tcctcgacga aattggcgaa atgccgctgg
     9361 tactgcaagc caaattacta cgcattctgc aggaacggga atttgaacgg attggcggcc
     9421 atcagaccat aaaagttgat atccgcatca ttgctgccac caaccgtgac ttgcaggcaa
     9481 tggtaaaaga aggcaccttc cgtgaagatc tcttttatcg ccttaacgtt attcatttaa
     9541 tactgccgcc tctgcgcgat cgccgggaag atatttccct gttagctaat cactttttgc
     9601 aaaaattcag tagtgagaat cagcgcgata ttatcgacat cgatccgatg gcaatgtcac
     9661 tgcttaccgc ctggtcatgg ccgggaaata ttcgagagct ttccaacgtt attgaacgcg
     9721 ccgtcgtgat gaactcaggc ccgatcattt tttctgagga tcttccgccg cagattcgtc
     9781 agccagtctg taatgctggc gaggtaaaaa catcccctgt cggagagcgt aatttaaaag
     9841 aggaaattaa acgcgtcgaa aaacgcatca ttatggaagt gctggaacaa caagaaggaa
     9901 accgaacccg cactgcttta atgctgggca tcagtcgccg tgcattgatg tataaactcc
     9961 aggaatacgg tatcgatccg gcggatgtat aacgccaaaa cttgctatgc agaaaattgc
    10021 acagtgcgca attttctgca tagccgctca ttatccttat aaatccccat ccaatttatc
    10081 ccttcatatt caattagtta aataacaaaa tccagtaatc tcattctggc actccccttg
    10141 ctattccctg actgtaccca caacggtgta tgcaagaggg ataaaaaatg aaaacaaaat
    10201 tgatgacatt acaagatgcc accggcttct ttcgtgacgg catgaccatc atggtgggcg
    10261 gatttatggg gattggcact ccatcccgcc tggttgaagc attactggaa tctggtgttc
    10321 gcgacctgac attgatagcc aatgataccg cgtttgttga tactggcatc ggtccgctca
    10381 tcgtcaatgg tcgagtccgc aaagtgattg cttcacatat cggcaccaac ccggaaacag
    10441 gtcggcgcat gatatctggt gagatggacg tcgttctggt gccgcaaggt acgctaatcg
    10501 agcaaattcg ctgtggtgga gctggacttg gtggttttct caccccaacg ggtgtcggca
    10561 ccgtcgtaga ggaaggcaaa cagacactga cactcgacgg taaaacctgg ctgctcgaac
    10621 gcccactgcg cgccgacctg gcgctaattc gcgctcatcg ttgcgacaca cttggcaacc
    10681 tgacctatca acttagcgcc cgcaacttta accccctgat agcccttgcg gctgatatca
    10741 cgctggtaga gccggatgaa ctggtcgaaa ccggcgagct gcaacctgac catattgtca
    10801 cccctggtgc cgttatcgac cacatcatcg tttcacagga gagcaaataa tggatgcgaa
    10861 acaacgtatt gcgcgccgtg tggcgcaaga gcttcgtgat ggtgacatcg ttaacttagg
    10921 gatcggttta cccacaatgg tcgccaatta tttaccggag ggtattcata tcactctgca
    10981 atcggaaaac ggcttcctcg gtttaggccc ggtcacgaca gcgcatccag atctggtgaa
    11041 cgctggcgga caaccgtgcg gcattttacc cggcgcagcc atgtttgata gcgccatgtc
    11101 atttgcgcta atccgtggcg gtcatattga tgcctgcgtg ctcggcggtt tgcaggtaga
    11161 cgaagaagcc aacctcgcga actgggtagt gcctggaaaa atggtgcccg gtatgggtgg
    11221 cgcgatggat ctggtgaccg gggcacgcaa agtgatcatt gccatggaac attgcgccaa
    11281 agatggttca gcaaaaattt tgcgccgctg caccatgccg ctcactgcgc aacacgcggt
    11341 gcatatgctg gtcactgaac tggctgtctt tcgtttcatt gacggcaaaa tgtggctcac
    11401 cgaaattgcc gacgggtgtg atttagccac cgtgcgtgcc aaaaccgaag ctcggtttga
    11461 agtcgccgcc gatctgaata cgcaacgggg tgatttatga ttggtcgcat atcgcgtttt
    11521 atgacgcgtt ttgtcagccg gtggcttccc gatccactga tctttgccat gttgctgaca
    11581 ttgctaactt tcgtgatcgc gctttggtta acaccacaaa cgccgatcag catggtgaaa
    11641 atgtggggtg acggtttctg gaacttgctg gcgtttggta tgcagatggc gcttatcatc
    11701 gttaccggtc atgcccttgc cagctctgct ccggtaaaaa gtttgctgcg tactgccgcc
    11761 tccgccgcaa agacgcccgt acagggcgtc atgctggtta ctttcttcgg ttcagtcgct
    11821 tgtgtcatca actggggatt tggtttggtt gtcggcgcaa tgtttgcccg tgaagtcgcc
    11881 cgccgagtac ccggttctga ttatccgttg ctcattgcct gcgcctacat tggttttctc
    11941 acctggggtg gcggtttctc tggctcaatg cctctgttgg ctgcaacacc gggcaacccg
    12001 gttgagcata tcgccgggct gatcccggtg ggcgatactc tgttcagtgg ttttaacatt
    12061 ttcatcactg tggcgttgat tgtggtgatg ccatttatca cccgcatgat gatgccaaaa
    12121 ccgtctgacg tggtgagtat cgatccgaaa ctactcatgg aagaggctga ttttcaaaag
    12181 cagctacaga aagatgcccc accatccgag cgactggaag aaagccgcat tctgacgttg
    12241 atcatcggcg cactcggtat cgcttacctg gcgatgtact tcagcgaaca tggcttcaac
    12301 atcaccatca ataccgtcaa cctgatgttt atgattgcgg gtctgctgct acataaaacg
    12361 ccaatggctt atatgcgtgc tatcagcgcg gcagcacgca gtactgccgg tattctggtg
    12421 caattcccct tctacgctgg gatccaactg atgatggagc attccggtct gggcggactc
    12481 attaccgaat tcttcatcaa tgttgcgaac aaagacacct tcccggtaat gacctttttt
    12541 agttctgcac tgattaactt cgccgttccg tctggcggcg gtcactgggt tattcaggga
    12601 cctttcgtga tacccgcagc ccaggcgctg ggtgctgatc tcggtaaatc ggtaatggcg
    12661 atcgcctacg gcgagcaatg gatgaacatg gcacaaccat tctgggcgct gccagcactg
    12721 gcaatcgccg gactcggtgt ccgcgacatc atgggctatt gcatcactgc cctgctcttc
    12781 tccggcgtca ttttcgtcat tggtttaacg ctgttctgac ggcaacccta cagacagaag
    12841 gaataaaaaa tgaaaaattg tgtcatcgtc agtgcggtac gtactgctat cggtagtttt
    12901 aacggttcac tcgcttccac cagcgccatc gacctggggg cgacagtaat taaagccgcc
    12961 attgaacgtg caaaaatcga ttcactacac attgatgaag tgattatggg taacgtgttg
    13021 caagccgggt tggggcaaaa tccggcacgt caggcactgt taaaaagcgg gctggcagaa
    13081 acggtgtgcg gattcacggt caataaagtg tgtggttcgg gtcttaaaag tgtggcgctt
    13141 gccgcccagg caattcaggc aggtcaggcg caaagcattg tggcgggggg tatggaaaat
    13201 atgagtttag cgccctactt actcgatgca aaagcacgct ctggttatcg tcttggagac
    13261 ggacaggttt atgacgtaat cctgcgcgat ggtctgatgt gcgccaccca tggttatcat
    13321 atggggatta ccgccgagaa cgtggccaaa gagtacggaa ttacccgaga aatgcaggat
    13381 gaactggcgc tacattcaca gcgtaaagcg gcagccgcaa ttgagtccgg tgcttttaca
    13441 gccgaaatcg tcccggtaaa tgttgtcacc cggaagaaaa ccttcgtctt cagtcaagac
    13501 gaattcccga aagcggattc tacggctgaa gcgttaggcg cattgcgccc ggccttcgat
    13561 aaagcaggaa cagtcaccgc cgggaacgcg tctggcatta acgacggtgc tgccgctctg
    13621 gtgattatgg atgaatctgc ggcgctggca gcaggcctta cccccctggc tcgcattaaa
    13681 agttatgcca gcggtggcgt gccccccgca ttgatgggta tggggccagt acctgccacg
    13741 caaaaagcgt tacaactggc ggggctgcaa ctggcagaca ttgatctcat cgaagctaat
    13801 gaagcatttg ctgcacagtt ccttgctgta gggaaaaccc tgggctttga tcctgagaaa
    13861 gtgaatgtca acggcggggc catcgcgctc gggcatccta tcggtgccag tggtgcacgt
    13921 attctggtca ctctgttaca tgcaatgcag gcacgcgata aaaccctggg cctcgcaaca
    13981 ctgtgtattg gtggcggtca ggggattgcg atggtgatcg aacgattgaa ttaacagcag
    14041 aagccacctc cagtccatag gaggtggttt cacaggcctc ataaaaactc gtcagagtgt
    14101 aacttgagtc cttactgaaa caggattaga gcagaagatg ttcatttgta aatataattg
    14161 gagagacgaa cagcaattag agagagtcgc ctcctcccag ttatttgtat aattcataaa
    14221 caccatctca aattcctcac atcaaaatcc attacgttgc tggaaacagc aaaggttcag
    14281 gcttatttat ccgtctctgt taaaacatca gaaagcaacg taccgatggg caaaacgatt
    14341 acattcattg tcaataactt atatcatcca caaaaggccg cgaaacgttg cgctggttga
    14401 aggaaaatcg taagcgtcaa cagagcgccg tattgacact tatttatcgg tgataaacta
    14461 cgttccatgg cagcagttcg tcaacacggt tggagggcca ttccggcagt acgctcagga
    14521 tatggcgcag atacgcttct ggatcgatac cgttcaaccg acagctcccg attagtccgt
    14581 acagcagagc tccacgctcg cctccatgat cgttgccgaa gaacatgtag atggtatgac
    14641 ctttatatac tcactatagc attggtggga tattccagag gaggtcatta tgcattcgga
    14701 aaatattgca gcttacatcg ggctggatgt acataaagaa acgttggcgg ttgctattgc
    14761 tgcacctgaa cggcttggcg aagttcgtta ttatggtacg attaacaacg aagcccaggc
    14821 tgtacgtcga ttatttcaga aattgcaggg actttacgga aacattcttt cctgctatga
    14881 ggctggcccc tgtggttttg ggctatatca tcaactcaca gcgatgaata taaaatgcca
    14941 ggttattgcc ccttcccgga taccaaaatc accgacagac agaatcaaaa atgatcatcg
    15001 ggacgcaatt tctcttgcca ggttactgcg ggctggtgaa cttacccctg tctggatccc
    15061 tgacctgact catgaagcta tgcgggatct aatcagagcc cgtgcagcag ctaaacgaga
    15121 cagcagagtt gcccgacagc gaattctgag catgctattg cgaacagaca aacgttatgc
    15181 agggaagcac tggaccggca aacatcgaac ctggttagcc aatcaatcat ttagtcagcc
    15241 atctcaacag attgcttttc aacactattg tcagagtctg gagcagattg aagatcgcat
    15301 acttcaactc gaccaggaaa tatcccgcct gttacctgag tggtctctgt gcaatctggt
    15361 ctgccagtta caggccctta aaggtgttgg tcagctaacc gcaataaccc tggttgctga
    15421 actgggtgat ttttcacgat tttccagtcc caaacaactc atggcttttc tgggactggt
    15481 tccaggggaa tattccagtg gaaacagcat tcgtcccagg ggaataacaa aagttggaaa
    15541 cagcgaactg agacgtctgc tttacgaagc cgcctggtct tatcgtacac ctgcaaaagt
    15601 tggtgcatgg cttatatatt accgaccgga ttctgtaaca caatattcca aagatattgc
    15661 atggaaagct caacaacgat tgtgttctcg ttaccgaagt ctgacagcaa aagggaaaaa
    15721 atcacaagta gccattacgg cggtggctcg tgagttgact ggatttatgt gggatattgc
    15781 acttgctgcc caatcatcat tcagtcagca gaagcaaaat taaaccctga agcagtcaga
    15841 cacggaatga gtgatcctcg taaaagaatt caggaggtca gaatgcgcca atacctgcat
    15901 atagaaagag gccagctcac tgcgtaaatc gtcatggggc taaaatcccc gaataagagt
    15961 ttgtaaaatc caagatacag tctgactgtt tcaggttctt taatccgcga tataacgaag
    16021 ctcccggagc tgcgtaaggt ctttttgggc tcaatatttg acaaaggcca taagaattct
    16081 ttttcccgag acagacggca cgaagcgctc tttctgctgt gttattgtcc gcctccgcca
    16141 gaccgtcatc actgtaataa cagagggcgt cccactgatt caggacatag ctgaacgctt
    16201 cgcccagtct ggattttttc gacaacgtgc cattcttctc caccatccat tcatgcagcg
    16261 acgtcagtaa cgctttgctt cgctgctgcc tggctgcaag acgttcagac tccggtaagc
    16321 cccgtatttc atcctcgatg gcgtacagtt cactgatgcg cttcagagct tcttctgccg
    16381 tcgtactttt gctgctgatg tatacatcgt ggatttttcg ccgggcatgg gcccagcacg
    16441 caacttctgt cagtgcacca ccttcacgtt cggcactgaa cagccgctcg taaccgctga
    16501 acgcatccac ctgcaggata ccccggaagg gacgaaggtg ttggtcccgg gactacttta
    16561 ggtcttggtt cactaataat ttcattactg tcaatagccg taatcgccat tgcaggaaat
    16621 aataaaaagt aagtgtactc tgactgcgta tgtatctact ttgtgtaatg caggtgtcta
    16681 ttgaaaataa ttcttttcgt gggcatctgt tgtataaaat aaaaaattat cgtaagtact
    16741 ccatctgcga tgtcttgtga aaattatcct gtctggcaac aatcgcgtcc atctgtatta
    16801 atggactcga acgatgaact ctcaaacaac actagatatc accggctttc gcacttattt
    16861 gccagactca ttacgtctca gattcgacga gcaattatct gtccggacta tcgcacagca
    16921 agttaagact cagttattcc cccattcaca atctctttca acgaattaac gcctcaggta
    16981 ttgtctgacc atgacccgag tccctttcat tcgcccaacc gataccatac tttatgccaa
    17041 cagaaagaat gaatgaaccg cgcctaaaat cagcgaggac acatggcaca aagtgcgaca
    17101 tgtctcacat ttaaccccag tgggggcaac aagtataaaa ccgttttttg attaagtggt
    17161 cggatatgaa aaggggaagg tttatgtgaa aaatacctgt gttttctttg aaaacacaca
    17221 acagtatcag gttatttatg tttatacagt gcgttccaaa ctgacctgaa taattacgca
    17281 agttgcaaca ataaaaacat ttttaccgta gtgtgaggta ctccggtaaa ttgctttcca
    17341 ggtgcagaca atgactaacg aagaactaca ggcaatcgca ttatccatgc ttgagcgcca
    17401 gcgcgaaaaa gtacaccagc aacgagagtc tttgcttaac ccatctccat tcaccaagcc
    17461 aaaacgattg gagcttgaag cattcttgga caaataccca cggcggctta aatcaggcaa
    17521 aaccagacca ccaggctgat agctcacagt ttgttctcct attcaataaa acggataaga
    17581 accatcagcg cggtagcgta aggcactaca gcgcattgct ttacgagtaa taacaatgga
    17641 tttatcacca gaagacgcaa agtttttacg agaactgcaa caccgtatgc tcagagaagc
    17701 cattatcgaa cagcaaacgg gcatgagcgc catagaccca ttgcgataca aacgggcctc
    17761 atctcctttg gttgacgaca attccaatgc ggataaaata cgtccaccac actgattaca
    17821 tttctcatta gcacagtggt gtgatgcagc atacgaggaa aaccggatga atgagaacag
    17881 aataaaaagg ctggagcaac tgttacaagc acgccaacgg gagtttgcca ctaaatgggg
    17941 acgtgccgcg ctgccacatg aacgcctaat ctattttcca cttgaagacc tcattaaaaa
    18001 taatggcaaa aaaatcaggc ctgataagaa acacttctta gaatgcaatg aagacaaata
    18061 acaacaatct gcatattgcc accaaccaca gatgtggcat cgttatcagc gcgcttgatt
    18121 ttctttatat ccactgacga gcttaccggg gcacctttgt ttgccggtca ttctggtatc
    18181 atgcagatat ttacggcgta caagcccact agcatgacat tgtaataagg cctgagccta
    18241 acgctttaat gtgtgtcata cactcagtgg cgtaatcgtt gacaatatgg tgcatgtaat
    18301 cgtaagcgtc cagcgaaatc catctggtca taatctgaag catccgacaa agtggtgtcc
    18361 accaaataag tgatctgcgc ccaatatcct gagccggaac tttgacgaag gccaacgaaa
    18421 aatgggggcg gatgttacag agttctcggt acaggggtaa agtggtacct gtcgccagtt
    18481 ctccgatctg tttaaccggg aaattatctc ctacagcttg tcagaaaggt cggtgatgga
    18541 gcatcgttaa tacgatgcta tacgatgcat tcacagtgcc cggccagagg atgccccgtc
    18601 gctgcatacg gatcagggtt ggcaatatcg aatcgcaggc tataggcaaa gttaaagccc
    18661 catggagtag cacaaaatat gccgcacaaa ggaaacggtc tgaataacgc agtgatggtg
    18721 aacttcttca gcacactgct aaaacgcata gtgatcgagc gctgattctg gcgaacaact
    18781 gaactaatac atcagaatct gcattatgtt aaataaatat gaaaagatgg tttaaatacc
    18841 ccgttacttt tgacttacac tatacggtat cgcatcgttt aatatccgca ccgaccagat
    18901 ttttatttct attagttgtc acaatactga atgcgtacga ccacagtatt ccggctcctg
    18961 tgtggtcatg ctttaattct gcgttccggg cagataagca gttgcttgca ggaatccttc
    19021 ttgtgttaat gtcagttccc ctttttacca gtgctgattt ccacattccg tccaacagag
    19081 cttatagcct tagcattgtc cggctgaagt tctttttgaa taataataga agcactgctg
    19141 gcagatccag tccgtttttc ataacccact gtactgataa ccataatcta atcagtagaa
    19201 attgagtcga aaataagcac tactccatac aggataatta gaggtcagtt tgattattca
    19261 caattcatca tcagcatttt ctgtttctga cgaaatcaac atgaaaagaa ccatataaga
    19321 taattattat aataacagct tta
//
LOCUS       DAZGVQ010000094        19247 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000094 DAZGVQ010000000
VERSION     DAZGVQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.095"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..408
                     /locus_tag="ACHLAG_003223"
     CDS             <1..408
                     /locus_tag="ACHLAG_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFP0656396.1"
                     /translation="DQSIDTGRFVLSRINNENYQFHHIRFDCIQGEQGELVFEPSDVE
                     YYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDGTNEIQYGKSNYYSFQHPHEG
                     SNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
     gene            complement(446..1345)
                     /gene="nhaR"
                     /locus_tag="ACHLAG_003224"
     CDS             complement(446..1345)
                     /gene="nhaR"
                     /locus_tag="ACHLAG_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFP0656397.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(1411..2577)
                     /gene="nhaA"
                     /locus_tag="ACHLAG_003225"
     CDS             complement(1411..2577)
                     /gene="nhaA"
                     /locus_tag="ACHLAG_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFP0656398.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGNVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3163..3315
                     /gene="mokC"
                     /locus_tag="ACHLAG_003226"
     CDS             3163..3315
                     /gene="mokC"
                     /locus_tag="ACHLAG_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFP0656399.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3419..4549)
                     /gene="dnaJ"
                     /locus_tag="ACHLAG_003227"
     CDS             complement(3419..4549)
                     /gene="dnaJ"
                     /locus_tag="ACHLAG_003227"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP0656400.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4638..6554)
                     /gene="dnaK"
                     /locus_tag="ACHLAG_003228"
     CDS             complement(4638..6554)
                     /gene="dnaK"
                     /locus_tag="ACHLAG_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFP0656401.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6931..7335
                     /gene="yaaI"
                     /locus_tag="ACHLAG_003229"
     CDS             6931..7335
                     /gene="yaaI"
                     /locus_tag="ACHLAG_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFP0656402.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7361..8074
                     /gene="msyB"
                     /locus_tag="ACHLAG_003230"
     CDS             7361..8074
                     /gene="msyB"
                     /locus_tag="ACHLAG_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP0656403.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8223..8789
                     /gene="satP"
                     /locus_tag="ACHLAG_003231"
     CDS             8223..8789
                     /gene="satP"
                     /locus_tag="ACHLAG_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFP0656404.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8824..9411)
                     /gene="mog"
                     /locus_tag="ACHLAG_003232"
     CDS             complement(8824..9411)
                     /gene="mog"
                     /locus_tag="ACHLAG_003232"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFP0656405.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9526..10479)
                     /gene="tal"
                     /locus_tag="ACHLAG_003233"
     CDS             complement(9526..10479)
                     /gene="tal"
                     /locus_tag="ACHLAG_003233"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP0656406.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10758..12188
                     /gene="yaaJ"
                     /locus_tag="ACHLAG_003234"
     CDS             10758..12188
                     /gene="yaaJ"
                     /locus_tag="ACHLAG_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFP0656407.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            12258..13034
                     /gene="yaaA"
                     /locus_tag="ACHLAG_003235"
     CDS             12258..13034
                     /gene="yaaA"
                     /locus_tag="ACHLAG_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFP0656408.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(13187..13483)
                     /gene="yaaX"
                     /locus_tag="ACHLAG_003236"
     CDS             complement(13187..13483)
                     /gene="yaaX"
                     /locus_tag="ACHLAG_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFP0656409.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13697..14983)
                     /gene="thrC"
                     /locus_tag="ACHLAG_003237"
     CDS             complement(13697..14983)
                     /gene="thrC"
                     /locus_tag="ACHLAG_003237"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFP0656410.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(14984..15916)
                     /gene="thrB"
                     /locus_tag="ACHLAG_003238"
     CDS             complement(14984..15916)
                     /gene="thrB"
                     /locus_tag="ACHLAG_003238"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFP0656411.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15918..18380)
                     /gene="thrA"
                     /locus_tag="ACHLAG_003239"
     CDS             complement(15918..18380)
                     /gene="thrA"
                     /locus_tag="ACHLAG_003239"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFP0656412.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18406..18525)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18461..18526)
                     /gene="thrL"
                     /locus_tag="ACHLAG_003240"
     CDS             complement(18461..18526)
                     /gene="thrL"
                     /locus_tag="ACHLAG_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFP0656413.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18740..>19247)
                     /locus_tag="ACHLAG_003241"
     CDS             complement(18740..>19247)
                     /locus_tag="ACHLAG_003241"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP0656414.1"
                     /translation="FPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKSSW
                     MSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQQP
                     AKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRLLH
                     DIEKNITK"
BASE COUNT         4647 a         5058 c         4705 g         4837 t
ORIGIN      
        1 gatcaatcca tcgacactgg acgatttgta ctgtctcgta tcaataatga aaattatcag
       61 tttcatcata taagatttga ctgcatccag ggcgagcaag gtgaattagt ttttgaaccc
      121 agtgatgttg aatattactt tgaaccagtg acgatacagg agtcaggaac cagcatatta
      181 aaaaatgagt tagaaaacag tgaggacggt gcgggtcaag taggctttca actctccaat
      241 gatggtacga atgaaatcca gtatggtaaa agcaattact actcttttca gcatccccat
      301 gaaggaagta atcagattcc tctatttatt cgcccgcgta catatggaaa taatgtctca
      361 agcgggcaaa taatgagccg agtcaaaatc gtggttatgt acaactaatc tatctcctcc
      421 actttaactt tgggggagct gccgattaac gcgccgctgg actaaaaagc gcagaataat
      481 ccgtattgca gattcgctgt accgccgggt gctgaatcat ccgctcagca aaaatagcat
      541 ggtactcttc catcacattc tcgacgcgac caatttctac gacagtttta tcggcataaa
      601 agtcatatgc ataaagcgtt ggggcaacga agattgcatt gtgcattgca ccaaaagctt
      661 tcatcaacgc ggcatcatca aactcgccga ggatttctac gtttaatccc tgggagttaa
      721 accagttaag caatttgcgc cctaacattg aacgtcgccc aggaatcaaa agtcgccgtt
      781 cttccagaca agccgggaac ggtttttctg gtggtggatt tgtacaccag aaactcacgc
      841 cacattcgcc aattctcacg gagaacaggc cttcctgctg cgtagagtct atcggacagt
      901 cggaaatgat catatccagt ttatgctggc ttaattgctc cagtagcatt tcgtgggtgg
      961 attcgaagca gcgaagatga atcggctcgc cttctaccac tgcagcattc agcacgctac
     1021 tgaccaggcg tttggaaagt gcatcagcca cgccaacgtc aaacaataaa ttggattctt
     1081 tgcgatagtt cacaatatcc agcatttcct ggcttaaggt gaacatttta tcggcatagc
     1141 gatagaccag ttctcccagc tcgctgggtt cgagaccacg tcccttgcgt ttaaataatt
     1201 tgccttgcag gcgctcttcc agcgctcgaa tctgtccggt aatggtttgt ggagttaaat
     1261 aaagcgcctc cgctgcgcca accacggaac cttctttata gacatgccag aagtaataca
     1321 agtggttgta attgatatga gacatgctca tttctctccc tgataacaat gaaaagggag
     1381 ccgtttatgg ctccccggta aaccgtcctg tcaaactgat ggacgcaaac gaacgcgtaa
     1441 ccagctgtat ccaattaccg ccgaagagat agaaccgacc aggataccga gtttcgccca
     1501 gttaatcagt tctggatcta cgttaccaaa ggccaggctg gcaataaaga tagacatagt
     1561 aaaaccgata ccgcacagga tccccaccgc cataatttgc tgataagtcg ttccctcagg
     1621 cagatgcgcc agtttcaaac gcagcgccaa ccagcagaac agactaatcc ccagcggttt
     1681 gccaatcagc aagccagcga tgatccccaa tggcagaatg gaggtcaagc catccagcgt
     1741 gacaccttgc agtgaaacgc cagcattagc aaatgcaaac agcggcaaaa tcagatacgc
     1801 cacccacggg tgtaacacat gctccagtcg cttcgctgga gaacgcccat gcttctcttt
     1861 caaaggaata aagaagccga caattacccc cgccagggtt gcgtgaaccc ccgatttcaa
     1921 caccgcagtc cacaacacta cgccaaccag aatatagacg cccgtgcggc gtacaccaca
     1981 cagattcaat accgcgagta ccgcaattgc tacagccgcg acgccaagag aggccatcga
     2041 taagtcatta gtgtagaaca atgcgatgat aatgatggcc ccaagatcgt cgataatagc
     2101 cagagccatc aaaaagatct tcagcgctaa cggaacacga cttcccaaca gcgccagcac
     2161 accaagtgca aaggcaatgt cagtagccgc cgggatcgcc cacccttcgc gggtaatcgg
     2221 atcggcatag ttaaaggcca gatagagtaa tgccggaaca atcatcccac caatagcggc
     2281 gataactgga aatgcggcct ggcgtaagct ggctagcgat ccttgcatca gttcacgttt
     2341 aacttccaga ccgactaaca ggaaaaatac cgccatcagc gcgtcattta tccataacag
     2401 catgtttttg ttgatttcga gtgaaccaac ccggagctga accggcgtct ccagaaagtc
     2461 gtgataccat ccactggttg cgccgctatt ggccataatc atcgccagga tagcggcaat
     2521 gataagaata atgcctcccg aggcatcact gctaaagaat cgatgcagat gtttcacttt
     2581 ttatttctct ttcaggtgaa tagatcgttt ttactatttt accccgcaca aatcatcgtt
     2641 aaaatcagat tatagtgtag gaataactcg ttttttacga tccattgaag aatttgcgcc
     2701 atcagcggtg agatgagttt ttgacggctg taagcgctca cgagtgaccc taagcccaga
     2761 taatcatagc ttaaggtgag gatttctgag agattaatta ggaaaagtaa taattaattt
     2821 gttgtcagtc ggcgatgttt gttaggccaa tatttcaaaa ataatccagc ttgtcgtcaa
     2881 taattgcgat gaagagataa attaaagtta aataattaaa tcattaaaaa attacgccat
     2941 aaaaatgcga ggcgtcttca ggattagaat cgataaaaca gaatgggtta aagagagcga
     3001 tataatagcg gcgggtgctt gaggctgtct gtctcaggca ttagctgaac ggcagataga
     3061 gaaaagcccc gagtgatatt ttaccatcaa cccgaggccc cctatatgct gaacacatgt
     3121 agagtgcctc ttactgaccg taaggtcaag gagaagagag caatgaagca gcataaggcg
     3181 atgattgtcg ccctgatcgt catctgtatc accgccgtag tggcggcgct ggtaacgaga
     3241 aaagacctct gtgaggttca catccgaact ggccagacgg aggttgctgt tttcacggct
     3301 tacgaatccg agtaagagca acggcgggga gtgatccccg ccactttaca ggtgctcgca
     3361 tatcttcaac gcaccctatt tttacccagg cctgcccacg ggcaggcttt tggggaggtt
     3421 agcgggtcag gtcgtcaaaa aacttcttca caccatcaaa gaagctcttt gagcgcgggc
     3481 tgttgtgctc gccggttggg ccaccgaagc tttcttgcag ctcttgcagc agctgtttct
     3541 gcttctcgtt caggcctacc ggtgtttcga cgacaacgcg gcacagtaaa tcaccctgtg
     3601 cgccaccgcg gacagacttg acgcctttac cgcgcatacg gaacagctta ccagtctggg
     3661 tttcgccagg cactttcagt ttgacgcgac catcaagggt cggtacttcg atttcgccac
     3721 ccagcgccgc catagcgaag ttgatcggga cttcgcaata caggttgttg ccttcacgct
     3781 cgaaaatcgg gtgctgttta acctgaacct gaacgtacag atcgcctgcc ggtgcgccat
     3841 gctcgcccgc ttcaccttcg cccgcaagac ggatgcggtc tccagtgtcc acccctgccg
     3901 ggattttaac ggacagcgtt ttgctgcgct caacacgacc atgaccatga catttgttgc
     3961 acggatcttt gatcagcgta ccgcgaccct gacagtgtgg acaggtctgc tgcacggcaa
     4021 agaaaccctg acgcatctgc acctgaccag aaccatgaca ggtcggacag gtctgcggct
     4081 gtgtacctgg ttttgcaccg ctaccgtggc aaacgtcaca ctcttccaga gtcggaatgc
     4141 ggatctcttt ggtcacgcca cgtacagctt cttcgagggt gagctccatg ttatagcgta
     4201 aatcagcacc gcgcgccgca cgttgacgac cacgtccgcc gccaaaaata tcgccgaaaa
     4261 cgtcaccaaa aatatcgctg aagtctgcgc cgccgccaaa accgccgccg cccataccac
     4321 cttgctcaaa cgcagcatga ccatactgat cgtatgccgc acgtttttgc gagtcggtca
     4381 gaacttcata agcttccttg atctctttaa atttcgcctc ggcctctttg tcaccctggt
     4441 tacggtccgg gtggtatttc atggccaggc gtttgtaggc ctttttgatt tcacgctctt
     4501 ccgctgtttt ggaaacgcct aaaatctcgt aataatcttg cttagccatc ttttttaaat
     4561 tgcccctaga tgaatgcacg ggcggagagg aaattcccct tcgcccgtgt cagtataatt
     4621 acccgtttat agggcgatta ttttttgtct ttgacttctt caaattcagc gtcgacaaca
     4681 tcgtcatctt tcgcgttgtt tgcagaagca tcagcaccgg cagtctgctg ctgggcatgt
     4741 tgctgctggg cgatttccat cagtttctgg gaaacctgtg ccagctcctg cattttcgct
     4801 tcgatagcgg ctttgtcttc acctttcaga gcagtttcca gtgcagtcag tgcagactcg
     4861 atagcagttt tgtcgtcagc cggcagtttg tcgcctgctt cttcaacctg cttacgggtg
     4921 ctgtgcagca gatggtcgcc ctggttgcga gtctgtacca gctcttcaaa cttacggtca
     4981 gcttcggcgt tagcttctgc gtcgcgtacc attttctgga tttcatcttc gttcagacca
     5041 gaagaagcct tgatggtgat cttctgctct ttaccgctgt ttttatcttt cgcggaaacg
     5101 tgcaggatac cgtcagcatc gatatcgaag gtaacttcga tctgcggcat gccgcgcggt
     5161 gccgggttga taccatccag gttgaactga cccagagatt tgttatcagc cgcacgttta
     5221 cgttcaccct gcagcacatg gatggttacc gcagactggt tgtcttcagc ggtagagaac
     5281 acctggctgt gcttggtcgg gatagtggtg tttttcgcga tcagcgtcgt catcacaccg
     5341 cccatggttt cgatacccag agacagcggg gtaacgtcca gcagcagtac gtctttcacg
     5401 tcaccagtca gaacaccacc ctgaacagca gcaccgattg ctacagcttc gtccgggtta
     5461 acgtctttac gcggctcttt accaaagaac tcagcaactt tcttctgaac cattggcata
     5521 cgagtctgac caccaacgag gataacgtcg tcgatatcag atacggacag gccagcgtcc
     5581 tgcagtgcaa ctttcagcgg ctcaatggaa cggtttacca gatcttcaac caggctttcc
     5641 agtttcgcac gagtcacttt gatgttcatg tgtttcggac cggtcgcatc tgcagtgatg
     5701 tacggcaggt taacgtcggt ctgctgagcg gaagacagtt cgattttcgc tttttctgcc
     5761 gcttctttca ggcgctgcat tgccagcgga tcgttgcgca ggtcaatgcc ctgatctttc
     5821 ttgaattctt caaccaggta gttgatcaga cggctgtcga agtcttcacc acccaggtgg
     5881 gtatcaccgt tggtagccag aacttcgaag gttttttcgc cgtcaacttc gtcgatttcg
     5941 ataatagaaa tatcgaaagt accaccaccc aggtcataaa ccgcgatagt acggttgcca
     6001 gtacctttgt ccagaccgta agccagcgca gctgcggtcg gttcgttgat gatacgtttt
     6061 acttccagac cagcgatacg gcctgcgtct ttggttgcct gacgctgagc atcgttaaag
     6121 tatgccggta cggtgataac agcttcagtt accggttcac ccaagtaatc ttcagcggtt
     6181 ttcttcattt ttttcagcac ttcagcagag atctgcggcg gtgccatttt ctggccttta
     6241 acttcgaccc atgcgtcgcc gttatcagca gcaataattt tgaacggcat gatggaaaca
     6301 tcacgctgta cttcttcgtc ctggaagcgg cggccaatca ggcgtttaat cgcaaacagg
     6361 gtgttttgcg ggttcgtcac tgcctgacgt ttagccggct gaccaaccag agtttcacca
     6421 tcctgggtat aggcaatgat agaaggcgtg gtgcgatcgc cttcggcgtt ctccagcacg
     6481 cgaggagtgg tgccatccat aatcgctaca caagagttgg tagtacccag gtcgatacca
     6541 attattttac ccatctaaac gtctccacta tatattcggt catcatgtgg ttgtgagtct
     6601 gtaatagggg cgaaacgtct ggtttcaact gcccaatttc atttttttgc agactcactc
     6661 actgcggttg actactaaat ggggtcgtaa cccacgtcat caagggggag atgcaaaaat
     6721 tttttgtgct tttgcgcaga aatttcgcga tatagcagga caagacgtga aactgacagc
     6781 aattttattc atataaagaa tgaatcgcag ccaagacatt catcaaaaaa ttgtaataaa
     6841 aagaaaacat tacgcacctg aatcttctct ttatcagcag taaactagtg ggtatccatc
     6901 cccctacctc tccccactaa gagaatcctt atgaaatccg tttttacgat ttccgccagc
     6961 ctggcgatta gtctgatgct gtgctgcacg gcgcaggcaa acgaccataa aatcctcggc
     7021 gtcattgcaa tgccgcgtaa cgaaaccaac gatctggcgc tgaaacttcc tgtttgccga
     7081 attgttaaac gcatacaact ctctgccgac catggcgatt tacagttaag cggtgcatcg
     7141 gtttatttca aagccgcccg tagcgccagt cagagcctga atattccttc agaaataaaa
     7201 gaagggcaaa ccactgactg gatcaacatt aacagcgata acgacaataa acgctgcgtc
     7261 tcaaaaatca ccttttccgg tcatacggtg aactcatcgg atatggccac gctgaaaatt
     7321 atcggcgacg actaacgcca gaatatagaa gccacaaaaa atgaatgtta attacctgaa
     7381 tgattcagat ctggattttc tccagcattg cagtgaggaa cagttggcaa atttcgcccg
     7441 attgctcacc cataatgaaa aaggcaaaac tcgcctctcc agcgtactga tgcgcaacga
     7501 actgtttaaa tcgatggaag ggcatcccga gcaacatcgc cgcaactggc agctgattgc
     7561 tggagaatta cagcattttg gtggcgatag tatcgccaac aaactgcgcg gacacggtaa
     7621 attgtatcgg gccattttgc tcgatgtttc aaaacgattg aagctgaaag ccgacaaaga
     7681 gatgtctacg tttgagattg agcaacagtt actggaacaa tttctgcgta acacctggaa
     7741 gaatatggac gaggaacata aacaggagtt tctgcacgcg gtcgatgcga gggtgaatga
     7801 gctggaagag ctgctgccgc tgctgatgaa agacaaatta ttggcaaaag gcgtgtcgca
     7861 tttgctttcc agccaactga cccgcatttt acgcacccac gcagcaatga gcgtacttgg
     7921 gcatggtttg ctgcgcggcg cggggctggg aggccctgta ggtgcggcac taaatggggt
     7981 taaagcggtc agcggcagcg cctatcgcgt gacgattcca gccgtactgc aaatcgcctg
     8041 cctgcgccgg atggttagcg ccactcaggt ctgataccgt aaactgggga aaattttcat
     8101 tttcccctca aaagatcgta gacactgccc cactggctga ttattatgcc gcgccctgaa
     8161 aacactacag ttattcaggg aaattatttc accattcatt cgatgatgat ttttgaggaa
     8221 ttatgggcaa cactaagttg gctaatccgg caccgctggg cctgatgggc ttcggcatga
     8281 ccaccattct gcttaacctg cacaacgtgg gttatttcgc tctggacggt attattcttg
     8341 ccatgggcat tttctacggc ggcatcgcgc aaatttttgc tggtctgctg gagtacaaaa
     8401 aaggcaacac tttcggttta accgcattca cctcttacgg ttctttctgg ctgacgctgg
     8461 ttgcgattct gctgatgccg aaactgggtc tgaccgatgc gccaaatgca cagttccttg
     8521 gtgtctacct gggtctgtgg ggcgtattta cgctgtttat gttcttcggc acgctgaaag
     8581 gcgcacgcgt tctgcaattc gttttcttta gcctgaccgt gctgtttgcc ctgctggcga
     8641 tcggtaacat tgccggtaac gccgcaatca tccactttgc cggctggatt gggctgatct
     8701 gcggtgccag cgcaatctat ctggcgatgg gtgaagtact gaacgagcag tttggtcgca
     8761 ccgttctgcc gattggtgaa tcccactaat tgttttgaga tccccccgct cggggggatt
     8821 tttttattcg ctaacgtcgc gtcttgcact cttcggtctg aatgctgcaa ccacttccgg
     8881 tgccgtttca acgtatggcc cttccagcaa ctgaatgcag tacggtacgc tggcaaaaat
     8941 accgtgtacc acaacgttac ccgcagcgtc cttcacacct tccagcgtct ctttaataga
     9001 cttcggctga ccgggtaagt taaggatcag cgcctgtttg cgaatcacac caacctgacg
     9061 cgaaaggatc gcagttggta caaaatgcag gcttatctgg cgcatctgtt caccaaagcc
     9121 aggcatctcg cggtccgcta ctgccagcgt cgcatcgggc gttacgtcac gacgcgccgg
     9181 gccagttccg cccgtggtga gcaccagatg gcaactcatt tcatccacta gctcacacaa
     9241 cgtttgctcg atgatcgcct gctcatcggg gattaagcgg gtttccagtt caaacggcgt
     9301 ggttagcgcc gatgtcagcc attcttccag cgcagggatg cctttatcct gataaacgcc
     9361 gctggatgcg cgatcagaga tggaaactaa gccaatacgt aaagtattca tcaagatttt
     9421 tccgtttaaa cagtctcgtt aaacagaatg atacactgcg aagggagtga cagacaggct
     9481 tcagaagagg tagcgtgacc gacttcccgg tcacgctaag aatgattaca gcagatcgcc
     9541 gatcattttt tccagttttt cctggtcaat agcaaactta cggatacctt ccgccagttt
     9601 atctactgcc attggatcct ggttgtgctg ccacaggaac tcggactcag tgatacgcgc
     9661 cggacgcgct ttcacttcgc cggtgtaaga cagtttacgt tcgatagccc cttcgctctc
     9721 agccagctct ttcagcagtg taggtgcgat ggtaagacgg tcgcagcctg ccagttccag
     9781 aatttcgccg atgttacgga agcttgcgcc cataaccacg gtttcataac cgtgctcttt
     9841 gtagtactgg tagatttcag atacagaaac cacgcccgga tcttctgccg gagcgtactc
     9901 tttcttatca gtattcgctt tgtaccagtc aagaatacgg ccaacaaatg gcgagatcag
     9961 gaacacgcct gcttccgcac aagcacgagc ctgagcgaag gagaacagca gggtcaggtt
    10021 acagttgatg ccttcttttt ccagctgttc tgcagcacgg ataccctgcc aggtagaagc
    10081 cagtttgatc agaatacgat cgttgctaat accagcatcg ttgtagagtt tgatcaggcg
    10141 ttttgctttc gcaattgacg cttcggtgtc ataggaaaga cgcgcatcaa cttcagttga
    10201 gatacgaccc ggaaccagtt tcaggatttc cagaccaata tttactgcca gtttgtcggt
    10261 cgcgtccacg atctgctgcg cgcgatcgtt gctctgctgt ttcgcccagg cgacagcatc
    10321 atcaatcaac ttacggtatt ccggaatctg cgctgcgtta agaatgagag aagggttggt
    10381 tgtggcatcc tgcggttgat acagcttcat tgccgcgatg tccccagtgt cggccactac
    10441 ggtggtgtac tgacgaaggg aggtcaattt gtccgtcatg atagtatttc tctttaaaca
    10501 gcttgttagg gggatgtaac cggtctgccc tgatgatatc acgacgcaac gtcagcgcaa
    10561 ccgccgacaa gacggttatc gccaggcgag actgtttcgg atttctgaca ctgcgttgat
    10621 atcgcccgcc atttttatac aaaacctcat gtatgctacg cagaagttat caagtacctc
    10681 gtagcgtata tacttcttaa acaattggta acgtttacac aggaaagtca tcgcgaccgg
    10741 caataagagg gatatgcatg ccagattttt tctcctttat taacagcgtc ctttggggat
    10801 cggtaatgat ttacctgctc ttcggcgcag gttgttggtt cacttttcgc accggatttg
    10861 tgcagtttcg ctacatccgc cagtttggca aaagtcttaa aaatagcatt catccacagc
    10921 caggcggttt aacctcattt cagtcattgt gtaccagtct tgcggcgcgc gtgggtagcg
    10981 gcaacctggc cggcgttgcg ctggcaatta ccgccggtgg acctggagcc gtcttttgga
    11041 tgtgggttgc cgcgtttatc ggcatggcga cctcgtttgc cgaatgttct ctggcacaac
    11101 tttataaaga acgtgacgtc aatggacagt ttcgtggcgg accggcatgg tatatggcgc
    11161 gcgggctggg gatgcgttgg atgggcgttc tgttcgccgt ctttttgctc atcgcctatg
    11221 gcataatttt cagcggagtt caggcgaacg ccgttgcccg cgccctaagt ttttcttttg
    11281 attttcctcc gctggtgaca ggcattattc tcgctgtctt tgctctgctg gcaatcactc
    11341 gcggtcttca tggcgtcgcc cggctcatgc aggggtttgt cccgttgatg gcgataatct
    11401 gggtgctgac cagcatggtg atttgcgtaa taaatatcgg gcaacttccc cacgtcattt
    11461 ggtctatttt tgagagtgct tttggctggc aggaagcggc aggcggcgcg gcgggatata
    11521 ccttaagcca ggcgattact aacggttttc agcgcagtat gttttccaat gaggcgggaa
    11581 tgggttcgac gccaaacgcg gcagcggcag cggcgtcctg gcctccgcat ccggcagcgc
    11641 aagggattgt ccagatgatt ggcattttta tcgacaccct ggtcatctgt acggcaagcg
    11701 ccatgctgat attactggcg ggtaacgaca caacctacat gccgctggaa ggtattcagc
    11761 ttatccagaa ggcgatgcgg gtgctaatgg gttcctgggg tgctgagttt gttaccctcg
    11821 tggttattct gtttgccttc agctccatcg ttgccaacta catttatgcc gaaaacaatc
    11881 tcttcttttt acgcctgaac aaccctaaag cgatctggtg tttgcggatc tgcaccttcg
    11941 caacggtcat cggcggcacc ttgctaagcc ttccgctgat gtggcaactg gcagatatca
    12001 taatggcctg catggctatt accaatttga ccgccatttt actgctctcg cctgtggttc
    12061 ataccattgc cagtgattat ctacgccagc gtaaactcgg cgtgcgcccg gtgtttgatc
    12121 cgttgcgtta tccggatatc ggtcgccagc tttctccgga cgcgtgggat gatgtttcgc
    12181 aggagtaatc acaactatcg atcaactcat tctctttttt tgctaaagtc ggcataaatt
    12241 tcctgcaagg actggatatg ctgattctta tttcacctgc gaaaacgctt gattaccaaa
    12301 gcccgttgac caccacgcgc tatacgctgc cggagctgtt agacaattcc cagcagttga
    12361 tccatgaagc gcggaaactg acgcctccgc agattagcac gctgatgcgc atcagcgaca
    12421 aactggcggg tatcaacgcc gctcgctttc atgactggca gccagatttc acgccggaga
    12481 atgcccgcca ggcgattctg gcgtttaaag gtgatgtcta caccggcttg caggccgaaa
    12541 ccttcagcga agacgatttc gattttgccc aacagcattt gcgaatgctt tccggcttgt
    12601 atggcgtact gcgcccgctc gatttaatgc agccttatcg tctggaaatg gggatccgtc
    12661 ttgagaatgc ccgagggaaa gatctgtatc aattctgggg agatatcatc accaacaagc
    12721 tgaacgaggc gctcgcagca caaggcgata atgtggtgat taatctggcg tcagatgagt
    12781 attttaaatc ggtgaagccg aagaaattga atgccgagat tatcaagccg gtgttcctcg
    12841 atgagaagaa cggcaaattt aagataatca gcttctacgc taagaaagca cgcggtctga
    12901 tgagtcgttt tattattgaa aatcggctga ccaaaccaga gcaactgact ggttttaata
    12961 gcgaaggtta cttctttgat gaagcttcct ccagcaatgg cgaactggtg tttaaacgct
    13021 acgagcagcg ttaagtttgt agtcaatgcc ggatgcggcg tgaacgcctt atccggccta
    13081 caaaagcatg cagattcaat atattgcaga attcacgtag gcctgataag acgcgccagc
    13141 gtcgcatcag gcgctgaatg ccggattgtt acccggcatt tgtcatttag cggtgatgtt
    13201 tgcctggacc atgaccgccg tgatgatcat gaggagcttt cttatggtgg cgcggcggtg
    13261 gcggcggtcc gtgtgggtgc cagcgattgc ctcgccattc ataatgttgt ttccaccagc
    13321 cgtggtcgcg ccagtgacct ccatcccagt aatagccacg attatcacga tcgcctatct
    13381 gtaattttac tgacgggact aacgtaattt ccgcagcctg tgctgccatg ggagcgacca
    13441 gaaccaggga aagtgcgagt acgatagatt gcatcttttt cacaggttac tccttttatc
    13501 attgcccgtt atgggtcgat atccggagat tacgcgatga gaacgacggt tgttattctc
    13561 cgcaatccta atctctaagt taccgcattt attgagaatt tctccttttt ccctgtcgtt
    13621 tttctccata atttcttcat aaaaaagccg ggctgcataa aagcaaaccc ggcctgattg
    13681 agataatgaa tagattttac tgatgattca tcatcaattt acgcaacgca gcaaaatcgg
    13741 cgggcagatt atgtgaaagc aagggtaaat cagcacgttc tgccagctct tttggcagat
    13801 ccaacgtttc accgagaatc gcttccacgc tctctttaaa tttcgccgga tgcgcggtgc
    13861 cgaggaacaa gccatattcg cctggattca actggtcacg cagcgcacga tacgctacgg
    13921 cagcgtgcgg ctccgaggtg tagcccagtt cttttaactc acgcattgtc tgttgcgtgg
    13981 tttcatcatc cacggctgca taacccagct ctttcagttg ccagattttg cggcggaaca
    14041 actcttccac acgcggccag ttgttcggct gactcacgtc catcgcgttg gataacgtcg
    14101 cctgagtcgc tttgggtgac cactgaccgt cgtgcaggaa acgtggcacg gtatcgttca
    14161 cgttggtcgc agcaataaaa cgtttcaccg gcagaccgag tgacttcgcc agcagacccg
    14221 ccgtcaaatc gccgaagttt ccgcttggca ccgagacaac cagctggttg cgcgcttcct
    14281 gcggcagctg cgcaacagct tcaaagtagt agcaaatctg cgccagcaaa cggctgatgt
    14341 taatcgagtt agctgagttt aaccccagcg ccactttcag ttcttcatca tcaaacgcct
    14401 gcttcaccag cgcctgacag gcatcgaaat cgccgtcgat ggcaacagtt tcgatattgc
    14461 cgcccaacgt acagaacagt ttttcttgca gtggactgat tttgcctcgt ggatagagga
    14521 taaccacttt cacattcggt aaaccgtaga aagcatgagc cactgccgct ccggtatcac
    14581 cggaggtcgc ggtcagaatg gtcactggct tatcgcccgc aatatgggtc agcatttgtg
    14641 ccataaagcg accgccgaaa tctttaaatg ccagcgttgg cccgtggaac aattccagac
    14701 aaccgacatc gctttcaaca ttggcgaccg gagccgggaa ggcaaacgcc gcgcgcacgc
    14761 gctcttccag gatttcctgc gggatttcat caccaataaa cgccgagagg atcttcgcac
    14821 tgcgggtgac aaaatccagc ttcagcatct catcaatttc agtcaggctg aattccggca
    14881 ggtcatgcgg aaaaaacagc ccctgatttt tgcccaaccc ctgggttacg gcttgcgcaa
    14941 agctgacctg ctcgttgtga tctttcagat tgtagagttt catttagttt tccagtactc
    15001 gtgcgcccgc cgtatccagc cggcaaatat gaacaaaacc ttcctgattt tgcaggtagt
    15061 tcttacccaa ccagtcggca acgcgctggg cggtatccgg cttgtcacat agagcgaaca
    15121 aggtcgggcc ggagccggag ataccgctcg ctaccgcgcc gatttccgcg accgcctgcc
    15181 gcgcctgccg gaagccaggc agtaaccgtt cacggtaggg ttcagcgata acatctttca
    15241 tcagcttcgc ggcaagctca ggctgacggg aatagcaggc gtgaatgaag ccagccagat
    15301 gtcgcccgtg cgcaatgcaa tcctggcggc gatactgcgc cggtaaaata gccctggctt
    15361 ctgccgtcga gactttaatc cccggatacg ccagcaccca cagccactca tcaaaccctg
    15421 gcacttgctg gctgatgatg tcgttttctt cgatcatcaa ctgcatacca ccaagaaaac
    15481 acggtgccac gttgtcgtaa tgaatgctgc cggagattcg tccttccagc tcgcccatca
    15541 aagccagcaa acgagtgtca ttaagcggct tgccacagtg ttcattcatc gccatcagcg
    15601 ccgcgaccac cgaacaggcg ctggagccta agcccgaacc gattggcata ttcttttcca
    15661 gagtcatcgc cactggaatt tgcttgccaa gctcctggca aaaacgctcc cagcactgat
    15721 aaacgatatt ttcccgtggt tctgacggca gcttatcggc aaagcgtccg aggttgttga
    15781 gactgaatgt ctctgccgcc tcaaccgtga ctacatctcc gagcaatgca ccatcaacag
    15841 gtgtcaccgc cgccccgagc acatcaaacc cgacgctcat attggcactg gaagccgggg
    15901 cataaacttt aaccatgtca gactcctaac ttccatgaga gggtacgtag cagatcggca
    15961 aagacaccgg cagctgtaac gtcattgccc gcaccatatc cgcgcagcac caacggcagc
    16021 ggctgataat agtggctata aaaggccagg gcgttttcgc catttttcac tttgaacagc
    16081 ggatcattac catccacttc ggcaatcttc acgcggcagg caccatcttc atcaatattg
    16141 ccaacatagc gcaaaacttt tccttcatca cgggccttcg ccacgcgcgc ggcaaagaga
    16201 tcgtcgagct gtgacagatt cgccataaaa gcggcaacat caccctcagc gttaaactct
    16261 gcgggcagca caggttcaat ttcaatatcc gccagctcca gttcacgtcc cgtttcacga
    16321 gcgagaatca atagcttacg cgctacatcc ataccagaaa gatcatctcg cggatccggt
    16381 tcggtataac ccatttcccg cgccagagta gtcgcctcgg agaaactcat gccttcgtct
    16441 aacttgccga agatataaga aagcgaacct gaaagaatgc cggagaactt catcaattca
    16501 tcaccagcat tgagcagatt ttgcaggttc tcaataaccg gtaatccagc cccaacgttg
    16561 gtgtcataga ggaatttacg ccgcgatttt tccgccgcat gacgcaacag atggtagtaa
    16621 tccatcgacg aggtgttggc ctttttgttc ggcgtgacaa cgtggaaacc ttcgcgcaag
    16681 aagtcggcat attgatccgc cactgcctgg ctggaggtgc agtcaacaat gaccgggttc
    16741 agcagatgat attctttcac gaggcgaatt aagcgcccga gattaaacgg ctctttggct
    16801 tgcgccagtt cttcctgcca gttttccaga tttaggccat gcacattggt gagcagtgcc
    16861 ttcgagttgg caacaccgca gacacgtaag tcgatatgtt tattcttcag ccagctttgt
    16921 tgacgcttca gttgctccag cagcgcaccg ccaacgccac cgacgccaat cacaaacact
    16981 tcgataacct gatcggtatt gaacagcatc tgatgagtaa cgcgcacgcc agtggtcgca
    17041 tcatcgttat ttaccacgac agagattgag cgttcagaag atccctgagc aatggcgaca
    17101 atgttgatat tggcgcgggc cagcgcggca aagaatttcg ccgagatccc acgcaaggtg
    17161 cgcataccat cacctaccac cgagataatg gccagccgtt ccgtcaccgc cagcggctcc
    17221 agtaagcctt ctttcagttc caggtagaac tcttcctgca ttgcccgttc agcccgcaca
    17281 cagtcgcttt gcggaacgca gaaactgata ctgtattcgg aagatgattg cgtaatcagc
    17341 accacggaaa tacgggcgcg tgacatcgcg gcaaagacgc gcgccgccat gccgaccatt
    17401 cctttcatcc ccgggccgga aacgctgaac attgccatgt tattcagatt ggaaatgccc
    17461 ttgaccggta attcgtcttc atcacgactg gcaccaatga gcgtacctgg tgcttgagga
    17521 tttccggtat ttttaatcag gcaagggatc tggaactggg cgatgggggt aatggtgcgg
    17581 gggtgaagaa ctttagcgcc gaagtaggaa agctccatcg cttcctggta ggacatcgac
    17641 ttcaacaacc tcgcatcggg cacctgacgc gggtcgcagg tatagacccc gtcaacgtcc
    17701 gtccaaatct cgcaacaatc ggcgcgtaaa caggcagcca gcaccgccgc ggagtagtcg
    17761 gaaccgttgc gtccaagtac caccagttcg cctttttcat taccggcggt gaaacctgcc
    17821 atcaacacca tgtgatcagc cggaatgcgg cttgccgcaa tacggcgggt ggactcagca
    17881 atatcgacgg tagattcgag gtaatgcccc actgccagca gtttttcgac cggatcgata
    17941 acggtaacgt tgtgaccacg cgcttctaac acgccggcca taatggcgat cgacattttc
    18001 tcgccacggc aaatcagcgc agcgttgatg ctatccgggc actgccccaa caaactaatg
    18061 ccatgcagga catgttttat ttgggcaaat tcctgatcga cgaaagtttt caattgcgcc
    18121 agcgggaacc ccggctgggc ggcggcgagt cccgtcaaaa gttcggcaaa aatacgttcg
    18181 gcatcgctga tattgggtaa agcatcctgg ccgctaatgg ttttttcaat catcgccacc
    18241 aggtggttgg tgattttggc gggggcagag aggacggtgg ccacctgccc ctgcctggca
    18301 ttgctttcca gaatatcggc aacacgcaga aaacgttctg catttgccac tgatgtaccg
    18361 ccgaacttca acactcgcat ggttgttacc tcgttacctt tggtcgaaaa aaaagcccgc
    18421 actgtcaggt gcgggctttt ttctgtgttt cctgtacgcg tcagcccgca ccgttacctg
    18481 tggtaatggt gatggtggtg gtaatggtgg tgctaatgcg tttcatggat gttgtgtact
    18541 ctgtaatttt tatctgtctg tgcgctatgc ctatattggt taaagtattt agtgacctaa
    18601 gtcaataaaa ttttaattta ctcacggcag gtaaccagtt cagaagctgc tatcagacac
    18661 tcttttttta atccacacag agacatattg cccgttgcag tcagaatgaa aagctgaaaa
    18721 atacttacta aggcgttttt tatttggtga tatttttttc aatatcatgc agcaaacggt
    18781 gcaacattgc cgtgtctcgt tgctctaaaa gccccaggcg ttgttgtaac cagtcgacca
    18841 gttttatgtc atctgccact gccagagtcg tcagcaatgc catgactcgt tcgcgtaaag
    18901 cttgcagttg atgttggtct gccgttgtgt cacttttcgc cggttgttgt attaatgttg
    18961 ctaattgata gcaatagacc atcaccgcct gccccagatt gagcgaagga taatccgcca
    19021 ccatcggcac accagtaaga acgtcagcca acgctaactc ttcgttagtc agcccggaat
    19081 cttcgcgacc aaacaccagc gcggcatggc tcatccatga agatttttcc tctaacagcg
    19141 gcaccagttc tactggcgtg gcgtagtaat gatatttcgc ccgactgcgc gcagtggtgg
    19201 cgacagtgaa atcgacatcg tgtaacgatt cagccagcgt cgggaaa
//
LOCUS       DAZGVQ010000095        19139 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000095 DAZGVQ010000000
VERSION     DAZGVQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.096"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            126..1322
                     /gene="yjgN"
                     /locus_tag="ACHLAG_003242"
     CDS             126..1322
                     /gene="yjgN"
                     /locus_tag="ACHLAG_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFP0656415.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKLWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
     gene            complement(1378..4233)
                     /gene="valS"
                     /locus_tag="ACHLAG_003243"
     CDS             complement(1378..4233)
                     /gene="valS"
                     /locus_tag="ACHLAG_003243"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFP0656416.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(4233..4676)
                     /gene="holC"
                     /locus_tag="ACHLAG_003244"
     CDS             complement(4233..4676)
                     /gene="holC"
                     /locus_tag="ACHLAG_003244"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFP0656417.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(4810..6321)
                     /gene="pepA"
                     /locus_tag="ACHLAG_003245"
     CDS             complement(4810..6321)
                     /gene="pepA"
                     /locus_tag="ACHLAG_003245"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFP0656418.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            6475..6525
                     /gene="ytgA"
                     /locus_tag="ACHLAG_003246"
     CDS             6475..6525
                     /gene="ytgA"
                     /locus_tag="ACHLAG_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFP0656419.1"
                     /translation="MSIIILATISTTQENK"
     gene            6588..7688
                     /gene="lptF"
                     /locus_tag="ACHLAG_003247"
     CDS             6588..7688
                     /gene="lptF"
                     /locus_tag="ACHLAG_003247"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFP0656420.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            7688..8770
                     /gene="lptG"
                     /locus_tag="ACHLAG_003248"
     CDS             7688..8770
                     /gene="lptG"
                     /locus_tag="ACHLAG_003248"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFP0656421.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(8889..10391)
                     /gene="yjgR"
                     /locus_tag="ACHLAG_003249"
     CDS             complement(8889..10391)
                     /gene="yjgR"
                     /locus_tag="ACHLAG_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFP0656422.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGNNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDVKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGVQASAEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(10469..11467)
                     /gene="idnR"
                     /locus_tag="ACHLAG_003250"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(10469..11467)
                     /gene="idnR"
                     /locus_tag="ACHLAG_003250"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFP0656423.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYQGNTL"
     gene            complement(11534..12853)
                     /gene="idnT"
                     /locus_tag="ACHLAG_003251"
     CDS             complement(11534..12853)
                     /gene="idnT"
                     /locus_tag="ACHLAG_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFP0656424.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGHYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(12918..13682)
                     /gene="idnO"
                     /locus_tag="ACHLAG_003252"
     CDS             complement(12918..13682)
                     /gene="idnO"
                     /locus_tag="ACHLAG_003252"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFP0656425.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(13706..14737)
                     /gene="idnD"
                     /locus_tag="ACHLAG_003253"
     CDS             complement(13706..14737)
                     /gene="idnD"
                     /locus_tag="ACHLAG_003253"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFP0656426.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTEMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGVMAEFPM
                     MTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            14954..15517
                     /gene="idnK"
                     /locus_tag="ACHLAG_003254"
     CDS             14954..15517
                     /gene="idnK"
                     /locus_tag="ACHLAG_003254"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP0656427.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKHYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            complement(15521..16540)
                     /gene="ahr"
                     /locus_tag="ACHLAG_003255"
     CDS             complement(15521..16540)
                     /gene="ahr"
                     /locus_tag="ACHLAG_003255"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFP0656428.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRVDWQWVIPLPENIDIKSAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQELLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            16736..16820
                     /locus_tag="ACHLAG_003256"
     tRNA            16736..16820
                     /locus_tag="ACHLAG_003256"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16770..16772,aa:Leu,seq:caa)
     gene            17428..17727
                     /locus_tag="ACHLAG_003257"
     CDS             17428..17727
                     /locus_tag="ACHLAG_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656429.1"
                     /translation="MAISAKKAAYKEKKAKAAELAKLRRQQKAALTRMIKKAREDGTF
                     DSLPDDIKAKCLKHEAKREKAAYFYNQWKEKQAYAARREEVAGIDELSLQHFKSI"
     gene            complement(18167..18706)
                     /locus_tag="ACHLAG_003258"
     CDS             complement(18167..18706)
                     /locus_tag="ACHLAG_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0656430.1"
                     /translation="MSNIGYKRVSTVDQNTDRQLAGVTLDKVFEDKLSGKDTNRPGLQ
                     ACLSYLREGDTLHVHSLDRLGRNTLDILALVEELNNRGVIVRFHKEGIIADITSAMGK
                     MMLTMLSAVATCERELMLERKREAQAVNPTKRGKGKAVDREGIKKALSDGLSVRKTAE
                     SFNVAPSTVQRIKKEEEEA"
BASE COUNT         4726 a         4812 c         4632 g         4969 t
ORIGIN      
        1 gcgacttata aagtaataaa gtttttgcca gcctattact gataagagta ttgtaacgtg
       61 gcagaacatt ttgctgtctg tatatataaa ttatatcaat ggatttttta attcagggaa
      121 tttttatggc tcaagttatt aatgaaatgg atgttccgtc ccattcgttt gtttttcatg
      181 gtacaggtga gagatatttt cttatttgtg tggtgaatgt gttgttaacg attataacgc
      241 taggtatcta tttgccatgg gcattaatga aatgtaagcg ttatctctat gctaatatgg
      301 aagttaacgg acaacgattt tcttatggaa ttactggtgg gaatgttttt gttagttgtc
      361 ttgtttttgt tttttgctat ttcgcaatct taatgacagt gtcagcagat atgccacttg
      421 ttggctgtgt tttgactttg ttactgttgg ttttgcttat atttatggca gcaaaaggac
      481 tgcgttatca ggccttgatg accagtctca acggcgtaag atttagtttt aattgctcta
      541 tgaaagggtt ctggtgggta acctttttct tgccgatttt aatggccatt gggatgggga
      601 ctgttttctt tatctcgaca aagatgctac atgccaatag ttcaagtagt gttattatat
      661 ctgtggttct gatggcaata gttggtattg tttccattgg tatttttaat ggtactttat
      721 atagcctggt aatgagtttt ctctggagca ataccagttt cggtatacat cgtttcaagg
      781 tgaaattaga tactacgtat tgtataaaat atgccattct cgcattttta gctttattac
      841 cttttctcgc tgttgctggt tatattatct tcgatcaaat attaaatgca tatgatagtt
      901 ctgtgtatgc aaatgatgat attgagaatt tacagcaatt tatggaaatg caacgtaaaa
      961 tgataatcgc gcagttaatc tattattttg ggattgctgt tagcaccagt tatttaacgg
     1021 tgtcgttgcg aaatcatttt atgagcaacc tgtcactgaa tgatgggcgt attcgttttc
     1081 gctcaacttt aacgtaccac ggtatgcttt atcgcatgtg tgcgttggtg gtgatatccg
     1141 ggattacggg cggtctggct tatccactgc tgaaattatg gatgattgac tggcaggcaa
     1201 aaaatacgta tttgctgggc gatttggatg accttccttt aatcaataaa gaagaacaac
     1261 cagataaagg cttcttagcc aggatttcac ggggaattat gccttcttta ccatttctgt
     1321 aagagataaa aaaggccgga gcatgctccg gccttcgttt tcattactgt gttttgatta
     1381 cagcgcggcg ataacagcct gctgttcaat cagcttcgct ttcgcttctg catagccttc
     1441 cagcttttca cgctctttcg cgatgaccgc ttccggtgcg cgggcgacaa agccttcatt
     1501 cgccagcttg ttctcgatac ggctgatttc accttcaatc ttcgccactt ctttcgccag
     1561 acgcgccagc tcatcttctt tgttgatgag gccagccatc gggatcagca gctctgcacc
     1621 gtcgatgatc ttagtaacgg aaaccggacc tttgtcatcg gcaggcagca cggtgatgct
     1681 ttccagacgc gccagcgttt gcaggaagcc acggttttca tttacgcgac gttctgcatc
     1741 cgcgctgcaa ccacgcagta gcagctccag cggtttgccc ggcgcgatgt tcatttctgc
     1801 acggatgtta cgtaccgcaa cgatcgcctg cttcagccac tctgtgtcgg ccagtgcggc
     1861 ttcatcaacc tgagatgcat cgtactgcgg gaacggctgc agcatgatgg tgtcggcagt
     1921 gataccgcaa agtactttca cacgctgcca gatggtttcg gtgatgaacg gaatgatcgg
     1981 atgcgcgagg cgcagcagac cttccagcac ggtcaccagc gtatggcgag taccgcgcag
     2041 ttcggcttcg gtgccaccgt tcattaccgg cttagtcagc tccagatacc agtcacagaa
     2101 ctggttccag gtgaactcat acagaatgcc tgcggcgata tcgaagcgga agctgtccag
     2161 cgcttcgcgg tacgctttga tggtctggtt gaactccgcc agaatccagc gatccgccag
     2221 cgacagcgtc atctcgccgc cgttgaagcc gcaatcctga ccttctgtgt tcatcagcac
     2281 aaagcggctg gcgttccaca gcttgttaca gaagttacgg taaccttcca ggcgcttcat
     2341 atcccagttg atgtcacgac cggtagacgc cagtgccgcc agggtgaagc gcagggcatc
     2401 ggtgccgtgc ggctcaatgc cgttcgggaa ctgtttctcg gtgcgcttac ggattttgtc
     2461 cgccagctgc ggctgcatca tattgccggt acgtttttcc agcagttctg gcagcgaaat
     2521 accgtcaacc atatccagcg ggtcgataac gttacccttg gatttggaca tcttctggcc
     2581 ttcgtcgtca cgaatcagac cggtcatgta aacagtgtgg aacggcacct gcggtttgcc
     2641 attttcatct ttgatgaagt gcatggtcat catgatcatg cgggcaatcc agaagaagat
     2701 gatgtcgaaa ccagatacca tcacgctggt tgggtggaac tgacgcaggg cgtcggtgtt
     2761 ttccggccag ccaagggtag agaaggtcca cagcgcagaa gagaaccagg tatcgagaac
     2821 gtcttcgtcc tgacgcagga caacatcagc accgaggtta ttttctttac gcacttcgtc
     2881 ttcgttgcgg ccaacataaa cgttacccgc ttcgtcatac catgccggga tacggtgacc
     2941 ccaccacaac tgacgagaga tacaccagtc ctgaatatcg cgcatccagg agaagtacat
     3001 gttttcgtac tgcttcggta cgaactgaat gtcgccgttc tcaaccgctt caactgccgg
     3061 tttcgccagg acatcggcac gcacgtacca ctggtcggtc agcattggtt cgatgaccac
     3121 gccgccacgg tcgccgtaag gaacggtcag gtcatgtggt ttaatttctt ccagcaggcc
     3181 gagtgcgtca accgcagcaa cgaccgcttt acgtgcagca aaacgctcca gtttctggaa
     3241 ttctgccggg atctcgctgg aataaacgtc agattcgtta cctttggtat cgaacacctg
     3301 ggcactttca cggatatcgc cgtcaaaggt caggatgttg atcatcggca gggcgtgacg
     3361 tttaccgact tcatagtcgt taaagtcgtg cgccggggtg atcttcacgc agccggtgcc
     3421 tttttccatg tcggcgtgtt cgtcgccaac gatcggaata cgacggttaa ccagcggcag
     3481 aatgacatat ttgccaatca gatctttgta acgcggatct tccgggttaa cggctacgcc
     3541 agtatcgccc agcagggttt ccggacgggt agtcgcaacc accagataat ctttaccgtc
     3601 tgcggttttc gcgccgtcag ccagcggata gcggatgtgc cacatcgaac ctttcgattc
     3661 gcggttttcc acttccaggt cagagatagc ggtgcgcagt ttcgggtccc agtttaccag
     3721 gcgtttgcca cggtaaatca ggtcttcttt atacagacga acgaaaactt ctttcaccgc
     3781 attggacagg ccttcgtcca tggtgaagcg ttcacgctcc cagtccacgg agttgccgag
     3841 acggcgcatc tgacgggtaa tggtgccgcc agattccgct ttccattccc agattttgtc
     3901 gatgaaagct tcgcggccgt agtcgtgacg ggttttacct tcttctgcgg caatcttgcg
     3961 ctcaacgacc atctgggtag cgatcccggc gtggtcagta ccgacctgcc acagggtgtt
     4021 tttgccctgc atgcgctgat agcggatcat ggtatccatg atggtttgct ggaaggcgtg
     4081 acccatatgc aaactgccgg tgacgttcgg cggcgggatc atgatgcaga aactttcctg
     4141 gctttcatcg ccattaggct taaagtagcc ctgcttttcc cagtgctcgt aaagcggctg
     4201 ttcgatatct tgtgggttat atgtcttttc cattatttcc aggttgccgt attcaggttg
     4261 aaaccagcca cgcggtaggc tttatagcgt tcgcgcgcca gttgtttcag agaatcttca
     4321 taaggaacga agtctaccac ttctgtgaaa gcggtggcaa aatctgcaaa gcttgttcgc
     4381 agactaatca atatatcgcg cgggctgctg ctgcgttttt gcggccaggc gatctccacc
     4441 ggcgcaccgc cgcgcggtcc ttctcccgct aaattatgcg gaacaaagct ttctgccgga
     4501 cgcgcccaca gggcttcatc cagccggtaa gcctgctttt catcttcaca ggcgatgagc
     4561 acgcgcttac cactgcgcca acgttctgcg gcaatttcac acaccagctg ctcaacggcg
     4621 cttaagccat cgacggtggt gtcattgtcc agaaggtaga acgtcgcgtt tttcataaat
     4681 ggggcttctt gtggtggatt taaatattgc tgcattcctc accctaaaag atgagcaatg
     4741 aatttctatt gcgctcgatc ggttccctct cccttaggga gagggttagg gtgagggtaa
     4801 aacgcaaact tactcttcgc cgttaaaccc ggcgcggttt aacaggaact gtgccagcaa
     4861 cgctaccgga cgaccggttg cgcctttcgc tttaccagaa cgccaggcgg taccggcgat
     4921 atccaggtgc gcccagttgt acttacgggt aaagcgtgac aggaagcaac ctgcggtaat
     4981 cgccccacca ggacgaccgc caatgttcgc catatcggca aaattggact ccagttgttc
     5041 ctgatactcg tcacccagcg gtaagcgcca tgcgcggtca ccggattgtt cagacgcggc
     5101 aatcagttca tgggccagcg gattgtggtt cgccatcaga ccagtaatgt gatgacccag
     5161 cgcgatcacg caggcaccgg tcagcgtcgc cacgtcaatc accgcttccg gctcaaaacg
     5221 ctcaacgtaa gttaacacgt cgcacagtac caggcggcct tcagcgtcgg tgttgagcac
     5281 ttcaacggtt tgaccagaca tggtggttaa cacatcgccc ggacgatagg cgcgaccgcc
     5341 tggcatgttt tcacaacctg ccaacacgcc gataacgtta atcggcagtt gtagctccgc
     5401 gaccatccgc atcacgccgt aaaccgctgc cgcaccgcac atatcgtact tcatctcatc
     5461 catgccttct gaaggcttga tcgagatacc gccggagtcg aaggttaaac ctttacccac
     5521 cagcacgatt gggcgtgcat cttccgacgc gttgcctttg tactcaatca ccgacatcag
     5581 cgattcgttt tgcgaaccct gaccgaccgc cagataggaa tgcatcccca gctctttcat
     5641 ctgctgttcg ccgataacgc gggtgataac attcttgctg tagctgtcag ccagctggcg
     5701 cgcttgtgaa gcgaggtaag cggcgttaca gatattcggc ggcatattgc caagatcctt
     5761 tgctgcttta atcccggcgg caatcgccag accgtgctgg atcgcgcgct caccgctggt
     5821 cagttcacgg cgggtcggca cgttgaacac cattttacgc agcggacgac gcggttcgct
     5881 cttgttcgtt ttcagctgat cgaaactgta gagcgtctct tttgccgttt cgacagcctg
     5941 acgcactttc cagtagttgt tacggccttt aacgtgcagt tcagtcagaa agcagaccgc
     6001 ttccattgag ccagtatcat tcagcgtatt aatggttttc tgaataacct gcttgtactg
     6061 acgctcatcc agctcacgtt ctttgccgca accaataagg agaattcgct cggaaagtac
     6121 attcggaaca tggtgcagca acaatgtctg ccccggtttt ccttccagtt cgccccgacg
     6181 tagcagggcg ctgatgtacc catcgctgat tttatcgagc tgttctgcaa tcggagaaag
     6241 gcgacgtggt tcgaagacgc ccacgacgat gcaggcactc cgctgtttct ccgggctacc
     6301 gctttttaca ctaaactcca tgcactacgc tcctgaatct taaagacaac ggcggtggct
     6361 acagatagaa ttgcaagctt tcgtaactca tgtccgctgt tgcgatgact tcgtgttaat
     6421 cttaacgtta ttacggcatt ggcacgtcag aacaaaatct gagaggtgaa tccgttgagt
     6481 ataattatct tagcgacgat ttcgacgact caagagaata aatgacgttt aagccatgaa
     6541 acaagctaaa atcctgcaaa agacgagttt ttacgggcgt atttaaagtg ataatcataa
     6601 gatatctggt gcgggagacg ctcaaaagcc agctggcgat actcttcatc ttgcttttga
     6661 tcttcttctg tcaaaagtta gtgaggatcc tcggcgcagc ggttgacggc gatattccgg
     6721 cgaatctggt gctctccctt ctcgggttgg gcgtgccgga aatggcgcag cttatcctgc
     6781 cattaagcct gttcctcggg ctgctgatga cgctgggcaa actgtatacc gaaagtgaaa
     6841 ttacggtaat gcatgcctgc ggcctgagca aagcggttct ggtgaaagcg gcaatgatcc
     6901 ttgcggtatt cacggcaatc gttgcggcgg ttaacgtgat gtgggcggga ccgtggtcat
     6961 cgcgtcatca ggatgaagtg ttagcagaag cgaaagcgaa ccctggcatg gcggcgctgg
     7021 cgcaagggca attccagcaa gcgactaatg gcagttcggt gctgttcatc gaaagcgttg
     7081 acggcagcga tttcaaagat gtgttcctcg cgcaaattcg accaaaaggt aatgcacgtc
     7141 cttctgtggt ggtggccgat tccggacatt taacccagct gcgcgacggc tcccaggtcg
     7201 tcactctcaa ccagggaacg cgcttcgaag gcactgcact gttacgtgat ttccgcatta
     7261 cggacttcca ggattatcag gcgatcattg gtcaccaggc ggtggcgctc gacccgaacg
     7321 ataccgacca gatggacatg cgtacattgt ggaacactga caccgatcgt gcccgcgcag
     7381 aactgaactg gcgtatcacg ttggtattca ccgtgtttat gatggcactt atggtcgtac
     7441 cgttgagcgt ggttaaccca cgtcagggac gcgtactgtc gatgctgcca gccatgctgc
     7501 tgtatctact tttcttcctg atccagacct ccctgaaatc gaacggcggt aaaggtaagc
     7561 tggacccgac gctgtggatg tggaccgtta acctgattta tctggctttg gcgattgttc
     7621 tcaacctttg ggacactgtg ccggtccgcc gcctgcgcgc cagtttttcg cgtaaaggag
     7681 cggtgtgatg caaccttttg gcgtacttga ccgctatatc ggtaaaacta ttttcaccac
     7741 catcatgatg acgctgttca tgctggtgtc gctgtccggc attatcaagt ttgtcgatca
     7801 gctgaaaaaa gccgggcagg ggagttacga cgcgttaggc gcaggaatgt ataccttgct
     7861 gagcgtgccg aaagatgtgc aaatcttctt cccgatggcg gctctgcttg gggcgttgct
     7921 tggtcttggg atgctggcgc agcgcagcga actggtggtg atgcaggctt ctggttttac
     7981 ccgtatgcag gtggcgctgt cggtgatgaa aaccgccatt ccgctggtct tgctgacgat
     8041 ggcgattggt gaatgggtcg cgccgcaggg cgagcagatg gcgcgtaact accgtgcgca
     8101 ggcgatgtac ggcggctcgt tgctctctac ccagcaaggc ttatgggcga aagatggcaa
     8161 caacttcgtc tacattgagc gggttaaagg tgacgaagag ttaggtggca tcagcattta
     8221 tgcctttaac gagaatcgtc gtctacaatc cgtacgctat gccgctactg cgaagtttga
     8281 cccggaacat aaagtctggc gtctgtcgca ggttgatgaa tctgatttga ccaatccgaa
     8341 acagattacc ggttcgcaga cggtgagcgg cacctggaaa accaacctca cgccagacaa
     8401 actgggcgtg gtggcgctgg acccggatgc actctcaatc agcggtttgc acaactacgt
     8461 gaagtatctg aagtcgagcg gtcaggatgc cggacgttat cagctcaata tgtggagcaa
     8521 aatcttccag ccgctatccg tggcggtgat gatgctgatg gcgctgtcgt tcatctttgg
     8581 cccactgcgt agcgtaccga tgggcgtgcg tgtggtcacc ggtatcagct tcggttttgt
     8641 cttctacgta ctggaccaga tcttcggccc gctgacgttg gtttatggca tcccgccgat
     8701 catcggcgca ctgttgccaa gcgccagctt cttcttaatc agcctgtggc tgttaatgag
     8761 aaaatcgtaa ccaagcaagg gagcgaaagc tccctttcac attgttgata aagtcaaata
     8821 cgagttcgat cggtcccctc gcccctttgg ggagagggtt agggtgaggg gcacagaccg
     8881 cacctgactt accttcttct tccccccagc aaactcccca acatcccgcg tacaatctga
     8941 ttcgtcacct ggcgggcggc gcttttcgcc attgtttgca ccacaccatc tttcttcccg
     9001 ccgcgtggtc cggtagtgcc aaacaaaata tcctttaatc caccaagaat gccgtcatcc
     9061 accgccacct ctttcccttt cgcgggggga ttattttgct gctcggcgct tgcctgtacg
     9121 cctttttgca acatctcata ggcggattcc cggtccacct catcctcgta cttgccatac
     9181 accggagagt gattaatcaa gccattacgc tcatcttccg tcaccggtcc catccgcgaa
     9241 caaggcgcaa tcaccatcgc ccgttccacc actgaaggac ttcctttcac atcaagaaac
     9301 gagattaacg cctcgccggt ccccagttcc tggattgcct tttcggtatc aaatgccgga
     9361 ttggcccgca tggtttgcgc cgcagctttt accgctttct gatctttggg cgtaaaagca
     9421 cgcaaagcgt gttgaacgcg attaccgagc tgcccgagca cattatccgg aatatcagac
     9481 gggttttgcg aaacgaacca gacgcctacg ccttttgagc gaataagccg tatcacctgc
     9541 tcaatcttat ccagcagtac ctgcggtgca tcgttaaaca gcagatgtgc ctcgtcgaag
     9601 aaaaacacca gttttggttt ctccagatcg cctgcttccg gcaattgttc atacaactct
     9661 gaaagcatcc acagcaggct ggcggcgtac agtttcggca tctgataaag cttctcggcg
     9721 ctgaggatat tgataacgcc tttaccgttg gtatcggtgc gcatccagtc tttgatatcc
     9781 agcatcggct cgccaaagaa gtgtgctgcg ccttgctgtt ccagcgataa cagcccgcgc
     9841 tggatggcac caaccgatgc gctactaata ttaccgtact gattctggaa agatttggcg
     9901 ttattgccga tgtactgggt aatcgcccgc aaatctttaa agtcgagcag taacagcccc
     9961 tgatcgtcag caatgcggaa gatgatattc agcacgccag attgcacatc gttgagattc
    10021 aacagccgcg ccagcaacag cggccccagg tctgaaaccg tcgcccgcac cggatggcct
    10081 ttctcgccaa agatatccca caccaccacc ggattggcat gcggttgcca gtcattgacg
    10141 ccgatatttt taagccttgc gagcagtttt tccgacgccg ttcctgcctg cgcgataccg
    10201 gtcagatcgc ctttcacatc agccataaac accggcacgc cgatttccga caatgactcc
    10261 gccagttttt gcagcgtaac ggttttaccc gtccccgttg cgccagtaat cagcccgtga
    10321 cggttagcca ttcccggcag taaaaacagt tctgtgtccg gcgtgcgggc aattaacagg
    10381 ggttcgctca tgagatttcc tccagtttcc tgcctggagt ataggcaacg tccggcaaag
    10441 agaatacgtg taatgaagag aaaataacct aaagcgtgtt gccttgataa atctgatacc
    10501 cgaggtcaac agtgttgtgg ttgtgatcgt tatttttaat tttgctcagc aacatttggg
    10561 cggccatccg accaatatca aaacggggag taattacgct ggcgaggctt gggatcattt
    10621 gcctgccaat ctccagccca tgaaaacccg caatggaaat ttgttctggc acggcgaggt
    10681 ttcgctcgcg gcacagcaga agcgcaccca tcgcaatatc gtcattggtg caaaatacac
    10741 cgtctaaatc cgggtttgcg ctaagggcat cacgcattaa ctgcattcct aaatgaatgg
    10801 atgagatggc gcgtggattc atgcgtaatg gggaaagatt atgcagcatc atcgcatcgc
    10861 aatacccctg ataacgctgt tcatcgcggg tgtcatcttt ggaaccgaga tacagaattt
    10921 tgtgcctgac tcgcttctcc agcatggtac acaccatgtc aaaagcggcc tgccgattat
    10981 caaaaccgac ctccatatcc agccgttctc cctgtacatc catcaattcc acgaccggaa
    11041 tggtggctga acgcagaaat ttcaccgtcc tgatggtgtg atatttttcc gaaagtataa
    11101 tcccgtcaat gttataagag agcaggttaa tcaccgactc ttcttcggaa tcgcgatcgt
    11161 aattgtaatt agcaataagg gtctgataat tatgctcaga ggtgacggat tctattcccg
    11221 cgagaatgtc agcgaagagt tgattttgaa atgacggaat caagatacca agggtataac
    11281 tttgtgcatt caacaacatg ccgggagcgc gattagggat ataattgatc tcttccataa
    11341 ttttggcgat gcgctcgcct gtttcctttg ccaccttttt cggcgagcgg atataacgac
    11401 tcacggtcat ttttgttacg ccagccagcg tagcgatatc ctgtaaagaa attctgtgat
    11461 tgcgcataac ttctcccaac gtcgggggaa tcttcaatct tcccccgaaa ctacatcaca
    11521 atattttatt ctttcagtgc aatacggcgt taatggctaa cacgcccagc aaacccataa
    11581 tagaaataag ggtttccatc accgtccagg tacgcaacgt ttcaccaacc gtcagattaa
    11641 aatacccttt aaatagccag aagccagggt cgtttacgtg tgacgcgatc acgctgcccg
    11701 caccggttgc cagtaccatt aaagcgggat cggcatgggt aacgttgata atcggcaaca
    11761 ccacacccgc ggtggtaatg gccgcgacgg tggcagagcc cagagcgata cgcaacagcg
    11821 ccgcaacagt ccagcacatc aataacggcg aaagcgtagt tccggtcatt aagtgtgaaa
    11881 tatagtgccc gacaccgcta tctactaata cctgcttaaa cgcgccgccg ccagcgataa
    11941 taaacacaat catcgcgata gcgcctatag aatccccaat gatatccatg atttgctcga
    12001 tggtgcgtcc attacgtcgg cccagtgtga aaatcgcaat aacaatggca ataaacagcg
    12061 caacggcagg gttaccgaca aattcaaaga agaggcgcac ggtgttagtt ttcggtaacg
    12121 taatttcaca aacggcggcg atagccatca ggatgaccgg gatcacggca gcgaaaatac
    12181 tgttccagaa ggagggcatc tcctcttcgc taaacagatg aggattaaat aagccttccg
    12241 gtggtgcttt ctcaaagcga gttagcagtt tagaaaacag cggtcctgcg acaataactg
    12301 tcggaatggt aatgataaat ccatacagta aagtcgttcc gagattagcc tcaaagatag
    12361 tcgcgatggc agtaggccct ggatgtggcg gcagaaaaca gtgggttaca gagagcgctg
    12421 ctaccattgg tacgccaaca tacaacaggg gtaatcctga tgatgctacg atggtaaata
    12481 ccaacggcaa cagcaggaca aaacccactt caaaaaacat ggcgaggccc acaaccagac
    12541 cggtgatcac tagcgcccat tgcacgcgtt ttttaccaaa agtagcaatc agcgtagtgg
    12601 cgatacgttg tgccgcaccc gtatcagaaa tcagcttgcc taacatggcc ccgaaaccga
    12661 gaatcattgc cagcccgccg agcgtgctgc cgataccatt ttgtatagag tgcaggacgg
    12721 cctgtgcatc catcccttcg gcaaatccga cgacggcagc taccagaacg agggcaataa
    12781 agccgttaac tttaaagccg atcatcagga taagaagcag cgcgacgcct gccgcaataa
    12841 tgattaatgg catagtcttt atctcttatt agtgccagaa aagcagcgct ttcgcgctgc
    12901 acgtagggta gtaggtgtta aacagccact aacatgccgc catcaacaaa caacaggtgg
    12961 ccgtttacga aatcagaggc ttttgaagaa aggaacaccg cagcaccaat cagttcctgc
    13021 ggatctcccc agcgtgcggc gggggtccgt ttgcacaacc aggcggtgaa ggcttcgtcc
    13081 tcaaccagtg ctttagtcat ttctgttttg aaatagcccg gcgcaatacc gttgacctga
    13141 atattgtggc gcgccagctc gacgcacatg ccgcgggtga gcatttttac cgcccctttc
    13201 gatgcggcat aaggggtgat ggtgtcacgt cccagttcgc tttgcatcga gcaaatatta
    13261 ataactttac ctgccttgcg ttcaaccatg tgacgagtta ccgcttgcga taccaggaac
    13321 acggcggtct ggtttactgc gatcacatca ttccactctt gttcagggaa ctcagtaaaa
    13381 ggatgacggc gctggatacc ggcgttattc accagcacat caatggggcc gatgtccttt
    13441 tcgatatgtt caacggcggc atcaatttca tgtttatgag taacattaaa aggtgcggca
    13501 acggcctgaa taccctcctg gtggagtttt tctacagcaa gttctgcgcg ttcggcagta
    13561 atatcattaa taattatttg tgcgccatat ttacccaggc cggttgccag taaaaagcca
    13621 atgccctgtg ctgaaccggt aatcaagata ttttttcctg ccagtgaaaa tagatcgttc
    13681 attgtattat tcctttattt atctcttaga aaacaagctg gacttttgct gcctgggttt
    13741 tatcaccggc gaaacgtagc gcctcttcca ggtcagtgaa gggatattca gcactcagta
    13801 aaggcagtgg attgataacg ccattcgcca gccatgacac tgcggtatta aattcgctgg
    13861 taaaacggaa agagcctctg agtgaaatct ccttaccaat caacgtcatc attgggaatt
    13921 ctgccatcac gcctcccata cctacctgca ccattacgcc gcgtgcacga gtgacctcca
    13981 gacaggtatt cactgatgaa ggatgaccgg acacttcaaa gctgacatcg aaatagcctt
    14041 tttccgcttt ccagtgatcc atgtcgtcgt tttgtgggtt tacgagcaca tccgccccca
    14101 tctctttgcc cagcgaaagg gaacggggac tcacatcagc acagacaatt tccgcggccc
    14161 ccagtgtttt cactgcactg acaatcaggc agccaatggg tccaacaccg gaaataaata
    14221 ctcgcttgcc ctgtaactcg ccggcctgat gtgcggcatg aatcgcgacg gctaaaggtt
    14281 cggcaaaagc cataaccttt tcgtcagctt tggccggata agggacacat tgcgacgttt
    14341 cgaccatttt ataacgggta aaaccaccat caacatgagg gaaatacatg gcactgccaa
    14401 aaaaacgcat ctctgtacac tgattctcgt tatgttcaat gcagtatttg cagtgaccgc
    14461 acggtttaga cggattaatg gctaccgttt gcccttcatg taattctgat gagtcgctat
    14521 gaataacttt accgataact tcatgaccta acaccatcgg tgcctttatc atgaaattac
    14581 ctacttttcc ttcctgataa taatgtaaat cggaaccgca aattccacct cgggttattt
    14641 gtactaatgt tccattatta ttccaatcta tcgtctgctc ggtaacggca acagttttct
    14701 tgcccgcaac aacgcaggac tgtgttttca cttgcataaa ttcctcactg gtcaggtaga
    14761 cacctcggaa gcatttaagc ggttttaatt gtcattattt gtgatgaaga tcacgtcaga
    14821 aaattgttac attactatgt tacgcataac gtgatgtgcc ttgtaattct tatcagcaga
    14881 aataaaaaaa cgtgaaatta ttatgccacc aggcgtagta tcgcagcagg taagatgatt
    14941 caggagattt taaatggcgg gtgaaagctt tattttgatg ggcgtttcag ggagtggtaa
    15001 aacattaatt ggtagcaagg ttgccgcgtt attatctgct aaatttattg atggtgacga
    15061 tcttcatcca gccaaaaata tagataaaat gtcgcagggt attccattat ctgatgaaga
    15121 tcgacttccc tggctggaac gcttaaatga tgcttcatac agtctttata aaaagaatga
    15181 aacaggattt attgtctgtt catcattaaa aaaacattat cgtgatattt tacgcaaggg
    15241 tagcccccat gttcatttcc tctggttaga tggcgactat gaaactatcc tcgcgcgaat
    15301 gcagcgtcgg gctgggcatt ttatgccggt agcgttacta aaaagtcagt ttgaggcgct
    15361 ggagcgtcca caagcagatg aacaggatat tgtgcgcatt gatatcaacc atgatattgc
    15421 caatgtcacc gaacaatgtc ggcaggctgt gctggcgata cgacaaaaca gaatatgtgc
    15481 gaaagagggc agcgcctcag atcagcgctg cgaatgattt tcaaaaatcg gctttcaaca
    15541 ccacgcggta acgcgcctta ccgtcgcgca catgctggat ggcgtcgtta attttcgaca
    15601 tcgggaacag ttcggttgtc ggcgcaacct tgctgcgggc ggcaaagcgc atcagcttac
    15661 gcagttcata aggcgtgccg gtagcagagc cagagacgct gcgatcgccc gcaattaacg
    15721 taaaggccgg aacagacagc ggcgtgagaa ccgcaccgac agtgtggaaa ttaccgccgt
    15781 acgtcagcgc ctcaaaataa ggctgccagt cgaggctgac gttcacggta ttgataatga
    15841 gatcaaactg ccccgccagt gctttcagtg cctgcggatc gcggctattc accactttat
    15901 cggcacccat cgccagcaat tcctgctctt tcgccggatt agaactaaag gccgtcacct
    15961 cacatcccat tgcgtgcaga agttttatag cgatatgccc cagcccgcca ataccaatta
    16021 ccccaacgcg gctggtagca gtgatatggt gcatcaacag tggtttaaag accgtgatac
    16081 cgccgcacaa cagcggcccg gcagatttaa tgtcgatatt ttccggcagt ggaataaccc
    16141 attgccagtc tacacgcaac ttctcggcaa aacctccgcg attcataatt gttggcaccg
    16201 caccttgctc acagttgatc tgatttccgc taatacaggc gtcgcagtga ccacagctac
    16261 gcgctgtcca gccaatcccg acacgctgac cgacctgcaa acctttatcc tgcgcggcac
    16321 tcccgagcgc caccacgcga ccaatcacct catgcccggc aaccagcgga tattgtgaaa
    16381 atccccattc gttatcgatc atcgacagat cggaatggca gatcccgcag taatccacct
    16441 gcacttcaac atcttgtggc ttcagctcac cgggatcgta ctcataaact tccagttcgc
    16501 cgcccgcttc ttttgcggca tagcttttta tcattgacat tttttggtcc ttctctggtg
    16561 tggtctggga agtgtagagc atggcaggga ggggtgcatg gagtgtgaca aaggttacac
    16621 atcgctgtac gcaatgctga aaatttcagc acttagcgag atgcgagcaa gctggcgctt
    16681 gcatggtggc gtgcgacagg tataatccac aacgttttcc gcatacctct tcagtgccga
    16741 agtggcgaaa tcggtagacg cagttgattc aaaatcaacc gtagaaatac gtgccggttc
    16801 gagtccggcc ttcggcacca ttaacgctaa tcaataacga tttttccgca aatcaagtag
    16861 ttaggatttt accccgacta actttgtatg tgaaaaatac taatctctta tcaagtacaa
    16921 aagccactgt gtttgttaat ggctttggta tgttatcaca cagtggcgag tagttcacag
    16981 gctcaacctc gtaacaagtt cctttgaagc tggaagacgt gccaccgtct ctaaatcgta
    17041 taccaaaact cttagcaaga tgttccacac ctggcgcggt gtccaatgat gctacacgtc
    17101 gtgagacgtt gcagaggata aacaggctaa atgatgggaa gagttttaag tgcaatagtg
    17161 gattaaagcc cacctcttca aaacctaact tgatcgggaa agaatcaagc tctagcacgg
    17221 tttcgtccac cggagtacaa cgggtgcctg ataagagaag aactgctaag acttcatagc
    17281 ttttgtgaaa aagccctttt tagtggatac gcctacggcg cttatttttt gggatagccc
    17341 atcaaaggcg ctttatggct cctattgtct aaaaataatc ctcctggtat tttaagcatc
    17401 taagatggtt tgaatgatgg gggatgtatg gcgatatcag caaagaaagc cgcctacaaa
    17461 gaaaagaaag caaaagccgc agaactagcc aaactacgcc gccagcaaaa agcagcatta
    17521 accagaatga taaagaaggc tagagaagac ggcacatttg acagcttgcc agacgatatc
    17581 aaagcaaagt gccttaaaca cgaagcaaag cgggaaaagg cagcatactt ctataaccag
    17641 tggaaagaga agcaagcata cgccgcacgt cgtgaagagg tggcgggtat tgatgaatta
    17701 tccctgcaac acttcaaatc aatttaaata ctccctgatt ctctaaaacg caacaggacg
    17761 cattctaacg ctgttttcat tgcttggtat agggatgtat aggtgatgtg tgtttaatgc
    17821 gtctgtgagc ttgttttgtg ccttattttg cattttcata atgcagaaga gaacacggaa
    17881 agccttaaaa ttaattattg gggtatttaa ggctattttc aggttattta agggtattaa
    17941 acttgattta attggttaaa atataaacag acagttctgt atcttattga tttataaggt
    18001 tatttttaaa taaaggtaaa taaaaaataa atagccataa attagctata aatacatctt
    18061 aatacctata tcccttccct tatggctaga ttgcgagagg tgcttaacgc gattttcagc
    18121 gccccccgat ctggaaaatc cagaatcccc aaatatcaag gaggcattac gcctcctctt
    18181 cttccttctt gatacgttgc accgttgaag gagcaacatt aaagctctcg gcggttttac
    18241 gcactgacag gccatcagaa agggctttct ttataccttc cctatctact gccttccctt
    18301 taccacgctt tgtaggattg acggcttgcg cttctcgttt acgttctaac atcagttcac
    18361 gttcacacgt tgccactgcc gacaacatcg taagcatcat ctttcccatc gcgcttgtaa
    18421 tgtctgcaat gatcccttct ttgtgaaagc gaacaattac accacgatta ttaagctctt
    18481 ccactaaagc aagaatgtct aaggtgttac gccctaaacg gtcgagagag tgcacgtgta
    18541 aggtgtcgcc ttctctcaga taggataaac acgcctgcaa tcctggacgg ttagtgtctt
    18601 taccagacag cttatcttca aagactttat cgagagttac tcctgcaagc tggcgatcag
    18661 tgttctgatc tacagtgctc acacgcttat aaccgatatt gctcataaaa tcctcttaat
    18721 gtatcgattt ggcactactt caatgcaaat gagtatatcg tattcatttg gtctgtttca
    18781 agccttatag gtacggaata ttacacgatt tagatgtatt catttggtat gcctaatgga
    18841 tacacagggg aatagattaa atggtgttct gtttatacag gtttatttgt tagatcaaca
    18901 atttggcatt tctaaacaga tgtttgaacg ttgccaaatg tcttgacaac acaagaaaag
    18961 ggcttgctat gccacgcatt acacgaaata aggctgtaaa aacaaaataa attttaaaat
    19021 ttgcacaaac aagaaaactc gttcagaaac aagcagaaca atttttagcc aacaatttct
    19081 gatggtgctc tgtaagccgc attacacaag gctttgcgaa agtgatgtaa aagtgaaga
//
LOCUS       DAZGVQ010000096        19085 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000096 DAZGVQ010000000
VERSION     DAZGVQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.097"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            222..527
                     /gene="gfcA"
                     /locus_tag="ACHLAG_003259"
     CDS             222..527
                     /gene="gfcA"
                     /locus_tag="ACHLAG_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HFP0656431.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            634..1278
                     /gene="gfcB"
                     /locus_tag="ACHLAG_003260"
     CDS             634..1278
                     /gene="gfcB"
                     /locus_tag="ACHLAG_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFP0656432.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1275..2021
                     /gene="gfcC"
                     /locus_tag="ACHLAG_003261"
     CDS             1275..2021
                     /gene="gfcC"
                     /locus_tag="ACHLAG_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP0656433.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2021..4117
                     /gene="gfcD"
                     /locus_tag="ACHLAG_003262"
     CDS             2021..4117
                     /gene="gfcD"
                     /locus_tag="ACHLAG_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP0656434.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAKSHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4163..5302
                     /gene="gfcE"
                     /locus_tag="ACHLAG_003263"
     CDS             4163..5302
                     /gene="gfcE"
                     /locus_tag="ACHLAG_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFP0656435.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5290..5736
                     /gene="etp"
                     /locus_tag="ACHLAG_003264"
                     /gene_synonym="yccY"
     CDS             5290..5736
                     /gene="etp"
                     /locus_tag="ACHLAG_003264"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFP0656436.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            5756..7936
                     /gene="etk"
                     /locus_tag="ACHLAG_003265"
     CDS             5756..7936
                     /gene="etk"
                     /locus_tag="ACHLAG_003265"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFP0656437.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8051..9349)
                     /gene="appA"
                     /locus_tag="ACHLAG_003266"
     CDS             complement(8051..9349)
                     /gene="appA"
                     /locus_tag="ACHLAG_003266"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFP0656438.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9429..9521)
                     /gene="cbdX"
                     /locus_tag="ACHLAG_003267"
     CDS             complement(9429..9521)
                     /gene="cbdX"
                     /locus_tag="ACHLAG_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFP0656439.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(9534..10670)
                     /gene="appB"
                     /locus_tag="ACHLAG_003268"
     CDS             complement(9534..10670)
                     /gene="appB"
                     /locus_tag="ACHLAG_003268"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP0656440.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(10682..12226)
                     /gene="appC"
                     /locus_tag="ACHLAG_003269"
     CDS             complement(10682..12226)
                     /gene="appC"
                     /locus_tag="ACHLAG_003269"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFP0656441.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12360..13217)
                     /gene="hyaF"
                     /locus_tag="ACHLAG_003270"
     CDS             complement(12360..13217)
                     /gene="hyaF"
                     /locus_tag="ACHLAG_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFP0656442.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQMSQRQPPLGEVLAVDLLNLHADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13214..13612)
                     /gene="hyaE"
                     /locus_tag="ACHLAG_003271"
     CDS             complement(13214..13612)
                     /gene="hyaE"
                     /locus_tag="ACHLAG_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFP0656443.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(13609..14196)
                     /gene="hyaD"
                     /locus_tag="ACHLAG_003272"
     CDS             complement(13609..14196)
                     /gene="hyaD"
                     /locus_tag="ACHLAG_003272"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFP0656444.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELARDQLPAAEQSALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14193..14900)
                     /gene="hyaC"
                     /locus_tag="ACHLAG_003273"
     CDS             complement(14193..14900)
                     /gene="hyaC"
                     /locus_tag="ACHLAG_003273"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFP0656445.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(14919..16712)
                     /gene="hyaB"
                     /locus_tag="ACHLAG_003274"
     CDS             complement(14919..16712)
                     /gene="hyaB"
                     /locus_tag="ACHLAG_003274"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFP0656446.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(16709..17827)
                     /gene="hyaA"
                     /locus_tag="ACHLAG_003275"
     CDS             complement(16709..17827)
                     /gene="hyaA"
                     /locus_tag="ACHLAG_003275"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFP0656447.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            18254..18341
                     /locus_tag="ACHLAG_003276"
     tRNA            18254..18341
                     /locus_tag="ACHLAG_003276"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18288..18290,aa:Ser,seq:tga)
     gene            18502..18693
                     /locus_tag="ACHLAG_003277"
                     /pseudo
     CDS             18502..18693
                     /locus_tag="ACHLAG_003277"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(18906..>19085)
                     /locus_tag="ACHLAG_003278"
     CDS             complement(18906..>19085)
                     /locus_tag="ACHLAG_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFP0656448.1"
                     /translation="NNDVPMDAETLIALSAAAEQAMFTKGMEIHVRQRTMKKELEKLT
                     SADEILAYRVGWAQE"
BASE COUNT         4669 a         5060 c         4937 g         4419 t
ORIGIN      
        1 tttaatttgt attttgctaa gatttatctt atgctcacaa gtcgtatttc cagaggggga
       61 tagttaacaa tcttatattt ccctctgcgc gcctttccta cccgcagtat atagtgcaag
      121 cggtatgagg aaagggagat ttcacaagga tgttatactc aaagtagaca atcctgatct
      181 caagcgtacg tattgtccat gcgatgaata aaggaaaagt tatgaaacac aagctttctg
      241 caatccttat ggccttcatg ttgacgacgc cagctgcgtt tgctgcccct gaagcaacaa
      301 acgggaccga ggcaacgacg ggtactactg gcaccacaac gaccactacc ggcgcaacca
      361 cgactgctac taccactggt ggcgtggctg ctggcgctgt cggcacggcg acagttgtcg
      421 gtgtggcaac ggctgttggt gtggcgacgt tggcagttgt tgcagcaaat gacagtggcg
      481 atggcggttc tcataacacc tcaaccacca cgtccactac acgctaatac tgctttaacg
      541 cgtcgaaatt aaagttaaat gatacccggt tgcgatattc gtaccgggct ttagagattt
      601 gcattttttt aattaacctt tcaaggatta aaggtgcgcc ctcttatttt atcgattttc
      661 gcactatttc ttgcgggatg tacgcacagc cagcaaagta tggtcgatac atttcgcgcc
      721 agccttttcg ataatcagga tatcaccgta gcggatcagc agatccaggc gttgccttat
      781 tccactatgt atttacgcct taatgaaggg caacgaatct ttgtggtact gggatatata
      841 gaacaagaac aaagcaaatg gttatcccag gataacgcca tgctggttac ccacaatgga
      901 cgtcttttaa aaaccgtcaa acttaataat aatctgctgg aagtgacaaa ttccgggcag
      961 gaccctctgc gtaacgcgct ggcaataaaa gatggcagcc gctggacgcg cgatattctc
     1021 tggagtgaag acaaccattt tcgctctgcg accctgagtt ctactttttc ctttgctgga
     1081 ttagagacgc tgaatattgc gggtcgcaat gtgctgtgta atgtctggca ggaagaggtg
     1141 acttccacgc ggccagaaaa acagtggcaa aacacattct gggtcgattc ggctactggc
     1201 caggttcgtc aaagtcggca aatgttaggc gcaggggtta ttcccgtaga aatgacgttt
     1261 cttaaacccg caccatgaat aaattacagt cgtatttcat tgccagcgta ctttacgtaa
     1321 tgacacccca tgcctttgcg caaggaacgg tgactattta tctgcctggc gaacaacaga
     1381 cactttccgt tggccccatg gaaaatgttg tccagttggt gacacaaccg caactgcgcg
     1441 atcgcctctg gtggcctggt gccttgctga ccgactctgc ggcgaaagct aaagcgctta
     1501 aagactacca acatgtcatg gcacagcttg cttcctggga agcagaagct gacgacgatg
     1561 tggcggcaac gattaaatcc gtgcgccagc agttactcaa tctcaatatc actggtcgct
     1621 taccggtcaa actggaccct gattttgtac gagtggatga gaacagcaat cccccgttgg
     1681 tcggggacta tacgctctat accgtacagc gtccggtgac tattactctg ctgggtgcgg
     1741 tttctggggc cgggcaattg ccctggcagg caggccgtag cgttaccgat tatctgcaag
     1801 atcacccccg ccttgcaggc gcggacaaga ataacgttat ggtgattacg cctgagggtg
     1861 agactgtcgt cgcccctgtt gccctgtgga ataaaagaca tgtggaacca ccgcctggca
     1921 gccagctttg gttgggcttt tctgcgcatg tactgcccga aaaatatgct gatttaaatg
     1981 accagattgt gtctgtttta acgcaacgtg ttcctgactg atgaagaaga attcttatct
     2041 tttaagctgc ctggccattg ccgtctccag tgcctgtcat gctgaagtat taacctaccc
     2101 ggatccgctg ggttcgtcgc aatcagactt tggcggcaca ggattgttgc agatgccaaa
     2161 tgcgcgcatc gcaccggaag gtgaattcag cgtcaactac cgggataacg atcaataccg
     2221 gttctactcc acctccgtgg cgctgttccc atggctggaa ggcaccattc gttatacgga
     2281 tgtgcgcaca cgcaaatata gccagtggga agatttcagc ggcgatcagt catacaaaga
     2341 caaatcattc gattttaaac ttcgcctgtg ggaagaaggt tactggctac cgcaagtggc
     2401 gtttggtaaa cgtgatattg ctggtacggg tctgtttgac ggtgagtatc tggtggccag
     2461 caagcaagcg gggccatttg atttcaccct cgggatggca tggggctacg ccggtaatgc
     2521 gggcaatatt accaacccgt tttgccgggt gagcgataaa tattgtcatc gcgcaaagtc
     2581 tcacgatgcg ggcgatatca gctttagcga tatctttcgt ggcccggctt ccatctttgg
     2641 cggcattgag tatcaaacgc cgtggaatcc cctgcgtctg aaactcgaat acgatggcaa
     2701 caattaccag aatgatttcg ctggcaaact gcctcaggca agccatttca acgtcggcgc
     2761 agtttatcgc gctgccagct gggcagatct caacctgagt tatgaacgcg gtaacacgtt
     2821 gatgtttggc ttcacgttac ggaccaattt caacgatctg cgccctgccc tgcgcgatac
     2881 gccaaaaccg gcatatcaac ctgcgcctga atctgaagga ttgcagtaca ccacagtagc
     2941 gaaccaactt accgcgctga agtacaacgc aggttttgaa gcaccggaaa ttcagctgcg
     3001 cgataagacg ctgtatatgt ctggtcaaca atacaaatac cgtgattctc gcgaagcggt
     3061 cgatcgtgcc aaccggattc tggtgaataa cctgccgcaa ggcgttgaga agattagcgt
     3121 gacgcaaaag cgcgagcata tggcgatggt gactaccgaa accgacgtag ccagcctgcg
     3181 caaacagctg gctggtacag cgcctggtca atcagagcaa cttcaacaac aacgtgttga
     3241 agcagaagat ctttctgcct ttggtcgggg ctaccgtatt cgtgaagatc gctttagcta
     3301 ctctttcaac ccaacacttt cacagtcgct gggcggcccg gaagatttct atatgttcca
     3361 gctggggctg atgtccagtg cccgctactg gtttaccgac cacctgctgc ttgatggcgg
     3421 tattttcacc aatatttaca acaactacga caagtttaag tcttcgctgt tgcccgcgga
     3481 ctctaccctg ccccgcgtgc gcacgcatat ccgtgattac gttcgcaatg acgtttatct
     3541 caacaacttg caggcgaact actttgccga cttaggcaat ggtttctatg gccaggtgta
     3601 tggcggttat ctggaaacga tgtacgccgg tgtcggttcc gagctgcttt atcgcccgct
     3661 agatgccagc tgggcgctgg gtgtggacgt taactacgtg aagcaacgtg actgggacaa
     3721 catgatgcgt ttcaccgatt attccacgcc aacaggtttc gtgacggctt actggaaccc
     3781 gccgacgctc aatggcgtac tgatgaaact tagcgttggg caatatctgg caaaagataa
     3841 aggcgcaacg atcgacgtcg ccaaacgctt tgacagcggc gtggcggtag gggtatgggc
     3901 ggcaatcagt aacgtatcta aagatgacta cggcgaaggc ggctttagta aaggttttta
     3961 tatctcgatt ccattcgact tgatgaccat tggacctaac cgcaaccgcg cggtggtttc
     4021 gtggacacca ttgacgcgtg atggtggaca aatgctgtca cgcaaatacc agctctatcc
     4081 aatgacggca gagcgagaag taccggttgg acaataacct gacgtaattc ataacattac
     4141 attgactgat aaatataaac caatgaaaaa gaacattttt aagttttcgg tactgacatt
     4201 ggctgttctg tctttgacag catgtaccct tgttcctggt cagaatttat cgaccagtaa
     4261 caaggatgtc attgaactac ctgacaatca gtatgacctg gacaaaatgg tcaatatcta
     4321 tcccgtcacg ccaggattga ttgatcagtt gcgcgccaaa ccgattatgt cgcaggctaa
     4381 cccggagctg gagcagcaaa tcgcaaacta tgaatatcgt atcggcattg gggatgtgtt
     4441 gatggttacg gtctgggatc acccggaact aaccaccccc gcagggcaat accgtagcgc
     4501 cagtgatacc ggcaactggg ttaacgccga tggtgcgatt ttctacccct atattggtcg
     4561 cctgaaagtg gccgggaaaa cacttaccca ggtacgcaac gagattacag cgcgtctgga
     4621 ttcagtcatt gaaagcccgc aagtcgatgt cagcgtcgcg gcgttccgct cgcaaaaagc
     4681 gtacgtcacc ggcgaagtca gcaaatcggg tcagcagccg attaccaata ttcctctgac
     4741 catcatggat gcgattaacg ccgcgggcgg tctgaccgca gatgcagact ggcgcaacgt
     4801 ggtgctcacc cagaatggcg taaagaccaa agtgaacctc tatgcgctga tgcagcgtgg
     4861 cgacttgcgc cagaacaaac tgctgcaccc gggcgatatt ttatttattc cgcgcaacga
     4921 tgatctgaaa gtcttcgtga tgggtgaagt gggtaaacag agcacgctga aaatggaccg
     4981 cagcggtatg accctggcgg aagcgctggg taacgctgag ggtatgaatc aggatgtcgc
     5041 tgatgcgacg gggatttttg ttattcgtgc aacgcaaaac aagcagaatg gcaaaatcgc
     5101 caatatttat cagctgaatg cgaaggacgc atcggcaatg atactcggaa cggaattcca
     5161 gcttgaacct tacgacattg tttatgtcac taccgcgccg ctggctcgtt ggaaccgtgt
     5221 gatttctctg ctggtaccga cgatttccgg ggtgcatgac ctgacagaga cttcacggtg
     5281 gattcaaaca tggcccaact aaaatttaac tcaatcctgg tggtttgtac cggcaacatt
     5341 tgccgttcgc ccattggcga gcgcttgctg cgtaaacgtc tgccgggcgt aaaagtcaaa
     5401 tcggcaggtg ttcatggtct ggtaaaacac cctgcagatg cgacagcggc agatgttgcc
     5461 gctaatcatg gcgtatctct tgaaggacat gccggacgta agctcactgc tgagatggca
     5521 cgaaattacg atctgattct ggcaatggag tcggaacata ttgctcaggt taccgcaatt
     5581 gctcccgagg tccgcgggaa gacaatgctg tttgggcaat ggctggaaca gaaagagatc
     5641 ccggatccct atcgtaaaag tcaggacgca tttgaacatg tctacggtat gttggagcgc
     5701 gccagtcagg aatgggcgaa gcgcctcagc cggtaatttg agtttataaa atacgatgac
     5761 aactaaaaat atgaatacgc caccaggcag cactcaggaa aatgagatcg atctgcttcg
     5821 tctggtcggc gagttatggg atcaccgtaa gtttattatc agcgtgaccg cgttattcac
     5881 gctgatcgct gtcgcttact cgctgttaag tacaccaatt tatcaggcag atactctggt
     5941 ccaggttgag caaaaacagg gcaacgccat tctcagcggc ctgagcgata tgatccctaa
     6001 ttcatcgccc gagtctgcac cggagatcca actgctgcaa tcgcgcatga ttctcggtaa
     6061 aaccattgct gaactgaatc tgcgcgacat agttgagcag aagtattttc cgattgtggg
     6121 tcgcggctgg gcgagattaa ccaaagaaaa accaggtgag ctggcgatca gctggatgca
     6181 tattccacaa ctgaatggtc aggatcagca actgacactc acggttgggg aaaacggcca
     6241 ctatacactg gaaggtgaag agttcaccgt caatggtatg gtcggccagc gtctggaaaa
     6301 agatggcgtt gcgctgacta tcgcggacat taaggccaaa ccaggaacac agtttgtcct
     6361 gagccagcgt accgaactgg aagcgattaa cgcattgcag gaaaccttta ccgttagcga
     6421 acgcagtaaa gaaagcggga tgctggaact taccatgact ggtgatgatc cccagttgat
     6481 tactcgtatt ctgaacagca tcgctaacaa ctatttgcaa cagaatatcg ctcgccaggc
     6541 ggcgcaggat tcacaaagcc ttgaattctt acagcgccag ttacctgaag tgcgcagcga
     6601 gctggaccag gcggaagaaa aactcaacgt ttatcgccag cagcgcgatt cggttgacct
     6661 taacctggaa gccaaagccg ttcttgagca gattgtgaac gttgataatc aactcaatga
     6721 gctgaccttc cgcgaggcag agatctccca gctgtataag aaagatcacc caacttatcg
     6781 tgcgctgctg gaaaaacgcc agacgctgga gcaagaacgc aaacgcctga ataagcgggt
     6841 atcggcaatg ccttccaccc aacaggaagt gttgcgttta agtcgtgacg tagaagcggg
     6901 ccgtgcggta tatctgcaat tacttaaccg ccagcaggag ttgagtattt cgaaatccag
     6961 tgccattggt aacgtgcgga ttatcgaccc ggcagtcact cagccgcagc cagtgaaacc
     7021 gaaaaaagcg ttgaatgtgg tgcttggttt tattcttggc ctgtttattt ctgtgggtgc
     7081 cgtgctggcg cgtgcgatgt tgcgtcgtgg tgtagaagcc ccggaacaac tggaagagca
     7141 cggcatcagc gtttatgcca ctatcccaat gtccgagtgg ctggataaac gcacccgtct
     7201 gcgtaagaaa aatttatttt ctaatcagca gcgccatcgt actaaaaata tccccttcct
     7261 ggcggtggat aacccggcgg attctgctgt ggaagccgta cgtgcgctac gaaccagtct
     7321 gcatttcgct atgatggaga cggagaataa cattctgatg atcaccggtg cgacgccaga
     7381 cagtggtaaa acgtttgtca gttcaactct ggcagcggtg atcgcccagt ccgatcaaaa
     7441 agtgttattt attgatgccg acttacgccg tggttattcg cataacctgt ttaccgtgag
     7501 taatgaacat ggcttgtcgg aatatctggc aggtaaagat gagctcaaca aagtgatcca
     7561 gcattttggc aaaggaggct ttgatgtgat tactcgcggt caggtgccac ctaacccgtc
     7621 tgaactgctg atgcgcgatc ggatgcgtca attactggaa tgggcgaacg accattacga
     7681 tctggtgatt gtcgatacgc cgccgatgct ggcggtgagt gatgccgcgg tcgtggggcg
     7741 ttctgttggc accagcctgc tggttgcgcg ttttggcttg aacaccgcca aagaggtgag
     7801 tttgtcaatg cagcgtctgg aacaggcagg cgtcaatatt aaaggcgcta tcctcaatgg
     7861 tgtgattaaa cgcgccagca ccgcttacag ttacggctat aactattacg gttatagtta
     7921 ctccgagaaa gagtaaaact gaaagtggcc ggatgcggcg tgaacgcctt ttccggtcta
     7981 cgcattagac agttcttcgt ttgtcatcag cctcagagca ttcaggtaac tgaatgctct
     8041 tttttatgca ttacaaactg cacgccggta tgcgtgcttc attcacgatt tgcgtaaaac
     8101 ctgccaacga acacatgccc tgcgcatttc gctcttcaca tcctgccagg gtcagtttca
     8161 cctctccggg cggcgtattt aatgacagcg gcgttttatc acgcatctgc tgtaaagtct
     8221 ggaagaccag cgaaacctga atccactggc tgttatcgct tagccgacgc cagcgttcaa
     8281 acaccagttc accacctggc ggcgtgttat ccggctgacc gggaagcgtc cagttgagct
     8341 ccagtgcgcc gccgagattt gccagattag tatcgtgtcc ggcaataaac agtactgaag
     8401 tgggtaatgt cacaccatac gcctgttttt gcggtggatg gggcgtcaac gctgccatga
     8461 tcaaatccaa taacggggtg gcgcggctgc gggcaacctc tggcgtgcgt tgtagcaaat
     8521 aaaattgcgc gttatgcaaa cttagcaagg tgttccactg gtgtgaatcg gtgatccttc
     8581 cccaccccgg ctccggcatt ccctgtgctt gttgcaggag aaatatctcc gtcagcattg
     8641 atgcgaggct taccgcaccg gttaatgaga cattgtcggc gctcaccttg agttccgatg
     8701 gtaatgcctg cgttaatgaa cagctttcgt cctgtttctc acgtttaagg cacaagtttg
     8761 attgcggaaa attaagcacc cgttccagtt cgcgaaacgc cgtttgccga tgcccggtaa
     8821 agtcagcaat tgaccctcct gccctgctga ggatcgcgtc agtcacgttc gcattatcca
     8881 gttggcaaac gccagttttt agaggattaa ataacggatc gggactggac gtatctgcct
     8941 gggtatgtac ggttattgca cagtcaggtg ccagcccggc ggcgaaggct tcgcctgttt
     9001 tacgggtacg ctcgtcgaca tcagcaataa tcgcgacctg accagactgc gggcagccct
     9061 ttttcgccag caatccgtcg gctaccagac gctggcgttg gtaatgtccg agataggcga
     9121 ttagctcacc accgcgcggt gtcagccaac ccagttttac cggccaggtt ggccatgcgt
     9181 ctggggtgac atcctgcatc agttgcgtgg ccttggttgg agcacgcaca ccatgacgac
     9241 tgacaatcac cacactttcc agcttcagct ccggctcact ctgagcgaat gcagattgcg
     9301 gggttaacgg aatcagaaga gataaaaatg ggattaagat cgctttcatc gatatgtttc
     9361 cgcttttcat atctgacatt attgattgcc tgatgcgatg ctaatgcgcc gcatcaggcc
     9421 tacgttcgtt aacttttcag acgcgggtcc agccatacca acacaagggt ggagagcgaa
     9481 cacatcaaca aaatgccgac gaaccaaagt aaataccaca ttgttcctgc tccttagtac
     9541 aactcgtttt cgttacggcg gagagtttct gttgtcatgc gcccccacat tttgtagtag
     9601 ctccagagag tgtagagcaa cacaatgggc aaaaatatca gcacgattac caacataatg
     9661 ctcagcgtca gctggctgga agtgctgtcc cacaacgtca ggctggagat cggactcacg
     9721 cttgacggca tgacgaaggg gaacagcgtg atgcctgccg tgaaaatcac gccaaattgc
     9781 atcaatgacg ccatcaaaaa tccccacccc gggcgaccac gataaatcgc catcaccgtc
     9841 agcaatgggc aaaagaaccc cagcagcggg aagatccaca aaacgggcga ttcgacgaaa
     9901 ttattcatcc aggcaccagg tagcactgcc accagtttca ttaacggatt ggaaggaccg
     9961 ttagcatcct gggcgagcag tacaaagcca tcaataccga cccacagcca gtaacccgcc
    10021 agcaaaaagc acagcatcac caacagtgca gcgcgtttag tcgccagctg tgaacgcaga
    10081 tgaatcacac caacagtttt cagttgtaac cagacgccac cttgcaaaat caccatccca
    10141 aggctgagca atccgcacaa taaagggaat ggcgtcagca gttgccagaa gctgccgaga
    10201 tactccacgc gtaattgcgg tgtgaaggca aacggcacgc cgagcaacaa gttgccgaag
    10261 gcgataccga agactaccgg cggcaccaga ctgccgatga ccagaccggc atcccacatt
    10321 ttacgccagc gtgcatcggc aatttttccg cgataatcaa aggccagcgg acggaagaac
    10381 agcgagcaca gcaccaggat catcgccaca taaaagccgg aaaacgccgc tgcatacact
    10441 ctgggccagg cggcaaataa tgccccacca gcgaggatca accagacctg gttgccttcc
    10501 cagtgtgcac caacgctgtt tatcactatc cggcgttcat catcattacg cgccaccagc
    10561 ggcagcagac agccgatccc catgtcaaat ccgtcggaga tcataaagac caccaggatc
    10621 acgccaatca gcagccacca gatgaagcgc aatgtttcat aatcaaacat gattttctcc
    10681 tttacccctg ttgctgcgtc ggttgttcac tctgcatcgc gctcggcccc agacgggcat
    10741 atttctgcat caggtagact tcggcgatta agaacagggt gtaaagccct acgatcatga
    10801 tcagtgagaa agccagttgt cctgtggtta aagcggagtg cgcggagtat gtcggtaaga
    10861 tgtcctgtat cgcccacggc tgacgaccaa actcggtcat aaaccacccg gcttcaatcg
    10921 caatccacgg caacggcaaa ctccagagcg ccattttcag cacccagcga tgctggtcga
    10981 ttttgccacg cagcgtctgg acaagcgcaa tcagcatcac cagtagcagc agggaaccac
    11041 agcccaccat gatgcggaaa ctccagaata ccggcgcaac ctgaggtatc gcgccacgca
    11101 tcgccgcctg gtactgtgcg gctgtgacat gattcatatc cggcgcatag cgggagagca
    11161 acatgccgta gcccaggtca ccttccagtt cccggaatgc ctgcaacaca tgcggctcac
    11221 gattgccttg cgatatttcc tgcattaaca gccaggccat acgtccgcgt tgcaagcgtg
    11281 ggtaggtttc agccatcaaa ttcttcagac ccggcacggg tttatctaat gagtgagtgg
    11341 cgaggatccc tagcagcgcg ggaattttga tggcaaaggc gttacgctct tgatcctgtt
    11401 ccggccaggc aaccacatgg aacggtgcag gtgcaggttc cgtttgccac tccccttcca
    11461 tcgccgccag ttttaccggt tgtacttgcg cgacttcata cgcagaactg tctccgagtt
    11521 gcagggtacc gataatcgcc agagtaccga agacggaacc gatggcaaac gaacgtaatg
    11581 cgacatcgcg ctcccgtccg cgcagtaaat accaggcgct gatcgccata ataaacatgg
    11641 ccccggtcac gtagcccgcc attacggtgt gcacaaattt cacctggctg accgggttaa
    11701 agaccagctc gctgaagctg gtcatctcca tacgcagggt gtcgatatca aaatgcgcac
    11761 cggtcgggta ttgcatccaa ccgttggcat tcaatatcca caacgcagag agatttgaac
    11821 cgaacgccac cagccacgtc accagcaggt gctggtattt attcagccgt tgccagccga
    11881 agaagaacag cccgacaaag gtggattcga ggaagaaggc cattaatgct tccatcgcca
    11941 gtggtgcgcc aaaaatatcg cccacatagt tggaatagaa tgaccagtta gtaccaaact
    12001 gaaactccat ggtcaggccg gtagccacgc caagagcaaa attgataccg aagagcttac
    12061 cccagaagcg cgtcatatcg cggtagattg ttttgccggt gaccacgtaa atggtttcca
    12121 tgatagccag caaaaaaatc agccccaggg taaggggtac aaataaaaag tgatacagcg
    12181 cggtcagagc aaactgccag cgcgataaat caatgacatc ccacatgcgc actcctgtag
    12241 gcgaagagag aaaaataacc cgataatggt agatctccct ctttatcctg aaactctacg
    12301 accagaggta ttcgccgatt gcgatattca taagactgaa agcgatactt accgtctttt
    12361 tacgtcggtg cagcttccgc cagccactga catacctcgc taagccgctg cgcagagtcg
    12421 accaaatctt ctggcgctgc cagcaccact tccggtatcg ggcagatttc ataactttcc
    12481 agtaaagggc cttttaaggt gtccgtacag cgtaaatgcc agacatggcg taaccccgtg
    12541 gcgttgatat agctctcgcc atagccaatg gtacgaatct gaatatttcc cggcccacag
    12601 agacgtgaga gaaaaagccg atcagcctcg ctgatgggta attgcgtcag attaatgctg
    12661 tgcggctgcg cgtcggggtt acgtacatgt gccagtaact catgcgccaa cggtaggcca
    12721 ttcatcaggc catcaatggg cggcggtaac agcgaatctg tcggcaagag attttgcgtt
    12781 gccgcctgcc acaacgccag cggcgcacag ccagcttcca gtttgtcctc cagcaacttt
    12841 tcgccgcgac gtctgcgcac ccgccataat ccgcagaaga tcgcctcctg tatttcactt
    12901 tcactgtcgt cagcctgctg aatgcgcact gacacttccc cttccccgag aagggtattg
    12961 ataaagtgac gatcgtcggc gtggagattt aacagatcga ctgccagcac ctcgcccaac
    13021 ggcggctggc gttgactcat ttgatgttgt aactcgttta acagcgcaat cacctgcggg
    13081 ctgtgagcac attgttccag ggccgccata ctcggttcat cattcacctg acaggtgatc
    13141 ggcagtggat tcatgctgaa actgtcatcg ttcggttgcg ttcctggccc cagcagatgg
    13201 aaaaaagttt cgctcatgag gcacgctcct gctgcggttc gacaagcccg cgcatcaggt
    13261 ttatcagttc cgcccacggg tgaataccat tcagcacacc gcgatagttt ccgccggtaa
    13321 acactaatgt ggcgggaaag cgaaagacgc caaagcgatc gccgatggct tcgctctgct
    13381 caaggtcagc aatcgccacc tgccatgtat agtcgggaaa ctcgcgcagt aattcgccaa
    13441 tcattaccgg attatcgcta acctctggcg tacgtttcgg gtcactgctt aataacacca
    13501 cgccgtctgg cgcttgcgta agccagtcgt caagacggga ttcactgacc ggcgtccagc
    13561 cgcgcgccag cattcgttgc cacaacgcat caaatggcgt gtcgttgctc atccctgctc
    13621 ctccagtgtc atcctgtact ggcgcaaacg aacgccttcg taattttcca tcgacagaca
    13681 gtcataattg agacagcgcg attcattagc cggttgcggc acaattcccc acgcagccag
    13741 ttgcgccaac gccgattgtt ccgcagcggg cagttgatcc cgtgccagtt cgctcaggct
    13801 gccgccgtag tcgtcgagca tagcgggttg cagaccgacg agggcaatat gtgctggcag
    13861 atgtccgcgg atatctgcca gcgccagcac ttcggagaaa ctgttctgat gcaggctcat
    13921 tttcttcgcg ctgagataag ccggaatgcg ttctccggca taggttcgca gcgttccagg
    13981 ttccagcccg tagtcaatgg catcgagaat caacagatgg ctggcgcttt cgacataccc
    14041 cagcaagttc agtccctgag taccgccatc gacaatctcc acatactcgg gccagtggta
    14101 atgggcatac agccgttccg ccacccgcac gccgaagcct tcatcggccc acagcaggtt
    14161 gcccagcccc atgaccacca cgcgttgctc gctcatgaac gctccttgtt acttattttg
    14221 ccaaatttgt ggctacggta gccgttgacc atggtggaga tcaccgtgtc gtcggacatg
    14281 atgtcttcac gcagcgccat gtagacatga ccgatcacaa acgcgccaat cagccacatc
    14341 cccagccgat gccagctgtg aatgtccatt gagttgccac ccgtccagta gaaaaattcc
    14401 accacataac ggaacggcgc aaaaatagcg tactggctgt gttcgctgta cagcgcaaaa
    14461 ccagtgatga tcataaagac cgacatcagg aaatagccga acatcgccgc ctgggcgatg
    14521 ggattatggc ctatatcggc actcggacgt tttgccagaa acagatacca gcggatttca
    14581 taccacacgc cctgccacca gcttttacgc cataccggca cgataaacag ctcgcgggag
    14641 tatcgattgc caacaaaagc ccagtagatc cgcatcagca aaaccacggt aaaaaccatc
    14701 ccggcgctga agtgaattaa cctgatgtag cccatataga acagatacgt cgcctcgccg
    14761 ctgacggaag gtagcggctt gccgataaag tatccggtga ccatcaacac cgccatgcat
    14821 aacaccgtca accagtgcca gatgcggact ggcgcttcaa agacatagtg gctgacaacg
    14881 ttgtcgcttt tctgttgcat gatgattctc cttcgctgtt aacgcacctg cacggagatc
    14941 agctcgctac cgtcgtcgcc cagcacgtgt gttgaacagg cgaggcacgg gtcaaagctg
    15001 tgcagagtac gcaggatctc cagcggttgc tcggggatcg ccattttggt gttcatcagc
    15061 gccgcttcat aagcgccaat ctgccctttg ggatcgcgcg ggctggcgtt ccaggtggtc
    15121 ggcaccacgc actggtagag atcaatcttg ccatcgcgaa tggcggccca gtggcctaac
    15181 gccccgcgcg gcgcttcggt aaaaccgaca ccacggcact ctgtcggcca ggttgcaggt
    15241 tcccattttt ccgtggaagc agtggcgaga ttgccgtttt tcaggttggt catcagcttg
    15301 tcgaagaaat actgcaactt acctgcggcc cactgcgctt cgtgcgcgcg gcacaaaatg
    15361 cggcctaacg ttgactggat accggaaagc ggcaggttca acgccgacat catgcgatcg
    15421 accgactcaa cggtcgcagc atcgcctttg tgataagcga ttaacgtgcg cgccagcggc
    15481 cccacttcca tcgcgttacc gcgccagcgt ggcgctttga tccacgagta gcgttcctgt
    15541 tcattcagct gctgaatgtt ggtatcgctg cctttgacat cgccggggtt gtaccacggg
    15601 tcggtgatgc catcgaacgg atgacgcccg acctgatcgt tgggatatcg ataccaggcg
    15661 tggtcgacaa actcctgcac ctgctgcgga tcaaccaaat ccactggcag cacattgttg
    15721 aagtcgccgt taatcaccgc gccgccaggc atcagcagac ttttctcgcc aaagtcgttg
    15781 gcaatatccg ggaatgcgcc gtagctgaga acgcatttat cagaaagacc agtgccgatt
    15841 tcgctccacg gtttgttgaa ctgaccgatg gctaaggcgt cggggatcat cacgttgtta
    15901 atgaagtccg ccgtgcgggt gataattgac tgcaccaggt tcaggcgttc catattgact
    15961 gccccgaccg cgccgctttc gtcaatgttg atggcgcaag gcatcccgcc gacaatccag
    16021 tttggatgcg ggtttttacc gccaaagacc gcgtggattt tgacaatttc acgctggaaa
    16081 tcgagagctt cgagatagtg ggcaaagccc atcaggttag cttctggcgg cagtttgtac
    16141 tgcgggtgcc cccagtagcc attgcggaag atccccaact gcccgccttc aacaaatttt
    16201 ttcaggcggt tttgtacgtc gaagaaatag ccaggggatg atttcggcca agaggagaga
    16261 ctttgcgcca gttcggaggt tttccgcggg tcggctttca gcgcatctaa cacatcgatc
    16321 cagtccatcc cggcaagctg atagaagtgc accagatgat cgtggcacca gagcgttgcc
    16381 agcataatgt tgcggatgat attggcgttg tccggcactt taataccgat agcatcttcg
    16441 atggcgtaaa ccgaagccag ggcgtgtacg ccagtacaga cgccgcagat acgttcaacg
    16501 aacgcccacg catcgcgcgg gtcgcgccct tgtaggatga tctccagccc gcgaaacatg
    16561 gtgccgcagg agacggcatt ggtgatcaca ttctgatcgt taatattcac ttcgcagcgc
    16621 atgtggcctt cgatgcgcgt aatcgggtcg accaccaggc ggcgtccggc attattgatg
    16681 gtgtatccct gagtttcgta ctgagtgctc atgcctgttt atcctcattg cctggctgat
    16741 gttcggtttc tgtaggttgc tggttatgac gtctgcgctg gtcaacggcg ctggcgactg
    16801 cgtgcacacc aacagccgct gccaccacgc caagcgcggt taaaccgacg gtatcggcgg
    16861 tggaatgagt acccatttgc ggaatatcga ccacgcggct gtagaacgaa ccgcgatccc
    16921 agaaaccatt ttccgcacag cccaggcagc cgtgaccaga ctggattggg aaagaaacgc
    16981 catcattcca gcgtgtggag gaacaggcgt tataggtggt aggccctttg cagcccattt
    17041 tgtacaggca gtaacctttg cgggcagcgt catcatccca actctggacg aactctccgg
    17101 cgtcgaagtg ggcgcggcga tagcatttat cgtggattcg ctgaccatag aacatcagcg
    17161 gacggcccat tctgtcgaca tctggcaagc gatcaaaggt caccatgtaa gtaatgatgg
    17221 cgctcatcac atccgggatc ggcgggcagc caggtacttt gataatgggt ttgtcggtga
    17281 tgactttgtc gataggcgtt gcctgcgtcg gattgggccg cgcggcctgc acgcagcccc
    17341 aggacgcgca ggttccccag gcgataatcg cgctggctcc ggcagcggca cgtttgagtt
    17401 tctcaataaa cggtcgaccg ctgctgatac agaacatccc ctgctcgccc agcggcggat
    17461 taccttctac tgcgaggata tatttgccat tgtattgcgt gatgatgtct tcgaagactt
    17521 cttccgcctg ggttccggcg gcagccatca aagtatcgtc ataatcgagg gaaatcaggg
    17581 aaaggatgac gtccttcgcc agtgggtgag cggagcggat aaaagattcg gtacagcagg
    17641 tgcattccag accgtggatc cataccaccg gaatgcgggg tttgttctcc agcgcccagg
    17701 caatctttgg tgccattccc gcgcctaatc ccagcgacgt ggcagccaga ctacaatatt
    17761 tgagaaagct gcgccgggta acgccctgac gccgcatggc ctggtaaaat gtttcctcgt
    17821 tattcatatc gcacgtctct tctccttgcg acaccggcag gacaccgggc gcttatggtc
    17881 ggcaagcgac gtccctgtag caaggcaaac catcgcttca cgtgagatgc tgaaaacgaa
    17941 agctcatcct tctgcactgg cacacgtcgc cagaaagtat tgttaataaa gcgtagtgaa
    18001 acttttgcac aaaacaatac aaactgtgtg gatttatctt ttagcgataa aaatggacct
    18061 atttttcttt tggccgggcg gtggggatgt ttagccggtt gctaaacgag taaaagagaa
    18121 ggaattcatg gtgatgaaat gatcaatctg ctgttatttc acgctatcaa tgcacttttg
    18181 gctgtttttc aggcaaacaa acaaattagg ggtttacacg ccgcatcggg atgtttatag
    18241 tgcgcgtcat tccggaagtg tggccgagcg gttgaaggca ccggtcttga aaaccggcga
    18301 cccgaaaggg ttccagagtt cgaatctctg cgcttccgcc aaacaatcaa ggggttacca
    18361 aatggtagcc cctttctttt tttgctctta gtatacactt ggtatactaa ttggtatatt
    18421 tagctcaaca cataccagat agatttttct ctttatgatt tatcgctata ccgatgaatt
    18481 atgaataacc ccaatccatg catgacgtgt ggtgcctgtt gtgcattttc cgcgtctctt
    18541 tttactgggc agaaactgac gatgctggcg gaaaggttcc ggtcagtcgc tctggcggga
    18601 actccgggtg aaaacgcata ttgctctgtt tacaaaaacc gtccgtccac atgcagggaa
    18661 ttcgcaatgt ccggcgaaaa tggcatcgcg aatgaagcat gtaaccgcgc acgggcaaaa
    18721 tacggacttc cggaaatctg aaaaatcatc catatcaaca ggatgctctg gaaatgagac
    18781 cggtatcact ttcaggatta acttttccgt ggcactcttt atctgtgtag ataaggtctc
    18841 ttcacaataa ttacataatc aacgcagttc atgggggagg tgatggtccc ccatcttttt
    18901 gtctgttatt cctgtgccca gccaaccctg tatgccagta tctcatccgc actggtcagc
    18961 ttttccagct ccttcttcat ggtgcgctgg cgaacgtgga tttccatccc tttggtgaac
    19021 atcgcctgct ctgcagcagc actcagagca atcagtgttt cagcgtccat cggtacgtca
    19081 ttatt
//
LOCUS       DAZGVQ010000097        18902 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000097 DAZGVQ010000000
VERSION     DAZGVQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.098"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            88..747
                     /gene="yqfA"
                     /locus_tag="ACHLAG_003279"
     CDS             88..747
                     /gene="yqfA"
                     /locus_tag="ACHLAG_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFP0656449.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(943..1923)
                     /gene="ygfZ"
                     /locus_tag="ACHLAG_003280"
     CDS             complement(943..1923)
                     /gene="ygfZ"
                     /locus_tag="ACHLAG_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFP0656450.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            2166..2432
                     /gene="sdhE"
                     /locus_tag="ACHLAG_003281"
     CDS             2166..2432
                     /gene="sdhE"
                     /locus_tag="ACHLAG_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFP0656451.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            2413..2820
                     /gene="ygfX"
                     /locus_tag="ACHLAG_003282"
     CDS             2413..2820
                     /gene="ygfX"
                     /locus_tag="ACHLAG_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFP0656452.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIGKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(2860..3381)
                     /gene="fldB"
                     /locus_tag="ACHLAG_003283"
     CDS             complement(2860..3381)
                     /gene="fldB"
                     /locus_tag="ACHLAG_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFP0656453.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            3493..4389
                     /gene="xerD"
                     /locus_tag="ACHLAG_003284"
     CDS             3493..4389
                     /gene="xerD"
                     /locus_tag="ACHLAG_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFP0656454.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            4414..5124
                     /gene="dsbC"
                     /locus_tag="ACHLAG_003285"
     CDS             4414..5124
                     /gene="dsbC"
                     /locus_tag="ACHLAG_003285"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFP0656455.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            5130..6863
                     /gene="recJ"
                     /locus_tag="ACHLAG_003286"
     CDS             5130..6863
                     /gene="recJ"
                     /locus_tag="ACHLAG_003286"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFP0656456.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            6954..8052
                     /gene="prfB"
                     /locus_tag="ACHLAG_003287"
     CDS             join(6954..7028,7030..8052)
                     /gene="prfB"
                     /locus_tag="ACHLAG_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFP0656457.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            8062..9579
                     /gene="lysS"
                     /locus_tag="ACHLAG_003288"
     CDS             8062..9579
                     /gene="lysS"
                     /locus_tag="ACHLAG_003288"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP0656458.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(9622..10170)
                     /gene="idi"
                     /locus_tag="ACHLAG_003289"
     CDS             complement(9622..10170)
                     /gene="idi"
                     /locus_tag="ACHLAG_003289"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFP0656459.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSTFAQHN"
     gene            10225..10296
                     /locus_tag="ACHLAG_003290"
     CDS             10225..10296
                     /locus_tag="ACHLAG_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656460.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(10293..10418)
                     /gene="yqfG"
                     /locus_tag="ACHLAG_003291"
     CDS             complement(10293..10418)
                     /gene="yqfG"
                     /locus_tag="ACHLAG_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFP0656461.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(10420..11868)
                     /gene="uacT"
                     /locus_tag="ACHLAG_003292"
     CDS             complement(10420..11868)
                     /gene="uacT"
                     /locus_tag="ACHLAG_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFP0656462.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVSKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            12304..14223
                     /gene="ygfT"
                     /locus_tag="ACHLAG_003293"
     CDS             12304..14223
                     /gene="ygfT"
                     /locus_tag="ACHLAG_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFP0656463.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQQIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            14223..14711
                     /gene="ygfS"
                     /locus_tag="ACHLAG_003294"
     CDS             14223..14711
                     /gene="ygfS"
                     /locus_tag="ACHLAG_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP0656464.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(14747..16114)
                     /gene="ghxQ"
                     /locus_tag="ACHLAG_003295"
                     /gene_synonym="ygfQ"
     CDS             complement(14747..16114)
                     /gene="ghxQ"
                     /locus_tag="ACHLAG_003295"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFP0656465.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYIGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(16150..17466)
                     /gene="guaD"
                     /locus_tag="ACHLAG_003296"
     CDS             complement(16150..17466)
                     /gene="guaD"
                     /locus_tag="ACHLAG_003296"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFP0656466.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(17484..18884)
                     /gene="xanQ"
                     /locus_tag="ACHLAG_003297"
     CDS             complement(17484..18884)
                     /gene="xanQ"
                     /locus_tag="ACHLAG_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFP0656467.1"
                     /translation="MSDINNAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
BASE COUNT         4798 a         4708 c         4991 g         4405 t
ORIGIN      
        1 tattttagct aacaggtgtt cactggaact attctcagtt acgctggaga ggtatacatg
       61 tgcgtgtacg tttctccgga gtaagttatg gttcagaagc ccctcattaa gcagggatat
      121 tcgctggcag aggaaattgc caacagcgtc agtcacggca ttgggttggt gtttggtatc
      181 gttgggctgg tgttgctact ggttcaggcg gtggatctca atgccagcgc cacagcgata
      241 accagctaca gcctctatgg cggcagtatg atcctgctgt tcctcgcttc gacgctctat
      301 cacgccattc ctcatcaacg ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt
      361 tacctgttga ttgccggaac ctacacgccg tttttgctgg tggggctgga ttctccgtta
      421 gcgcgcgggt tgatgattgt tatctggagc ctggcattgc tgggtattct gtttaaactg
      481 accatcgcgc accgattcaa aattttatct ctggtgacct atctggcgat gggctggctg
      541 tcgctggtgg taatttatga aatggcagtt aagctcgcgg cgggcagcgt taccttactg
      601 gcggtaggcg gcgtggttta ttcgctcggg gtgattttct acgtctgcaa acgcattcca
      661 tacaaccatg ccatctggca cggcttcgtg ctcggcggta gtgtgtgcca ctttctggcg
      721 atctatttgt atattgggca ggcgtaatgg gtatgccttt gtaggcctga taagacgcgc
      781 cagcgtcgca tcaggcatcg gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat
      841 ctacgggcct cgtccctgtt gtgataaagg catcaaatcc cagacatgat aagacgcgtc
      901 agcgtcgcat caggcgataa atgcctgatg cgatacagag atttactctt cgagcgaata
      961 cggcagcggc tcgatatgca gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg
     1021 ttccatatcg ttattcatga cgacctgtac cacgacctga ccatcttcca gttttaccgc
     1081 agccagcacc gtaccggtac gacgccagtt ctcgcccatt ttcagctcta agtcttcacc
     1141 agcttccggc agtcggctgg cgctacctgc cagcaaccag agcgcgcgtt tgttggcacc
     1201 acggaatttt gctcgcgcca ccatctcttg tccggtataa cagcctttct taaagctgat
     1261 accgcccagc gcctggagat tggtcgcctg tgggataaac tgcccgctgt tggcggcatc
     1321 aatcaccggg aaacccgctt caatgtttaa tgccagccac tgttggctat tgttcagttc
     1381 cgcttcaccg cgcagtttat cggtcagcat attagcagta gcttcatcgg ttacgatcag
     1441 gaaacgttct gccgggtgtt caaaccatag caaagtggtc gcgccttctt tgactacctg
     1501 tttttctttc gaaggcagtt cgctaaagag atttgccagc gcggcgcgcg cctgaaaacc
     1561 ggcaacacca agcagcacac gctcgtcgtc tggcgcgatg gtcactttag agaataccgc
     1621 atattttttc agttcagtca gctgcggttc acgcacgctg cgccgttcaa tccatgcaaa
     1681 gccatcgccg tcgcggaaca gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg
     1741 ggcggcgagc aggtgctgat cttctgccat ctggctgaca tctgctgtca cctgaccctg
     1801 catatatttt tcgctgtccg cgccagtaat ggtggcaagc gcccagtcat caagcgtcat
     1861 cagcgtcagc ggtaaacggg cagaagccgt aggctgacgg ggaggaaaag gtgtaaaagc
     1921 cataatagag tcctgagttg cttaacggcg tattaatgac taatggtaaa agagctatga
     1981 ggcaatgcaa gtgctttcac tacgcgatct gtgccttctg cgaggcgact tccaaaaaaa
     2041 tgaagccatt gaattgttgt gaattaattt cgcgatcgcg atggtgttta ctgatattcc
     2101 cgtaaaagaa ggcgaaaaac acgttacaat agcggggtac tggattttcg tgagacagga
     2161 agaagatgga cattaacaac aaagcccgca ttcattgggc atgccgccgt ggtatgcgcg
     2221 aactcgatat ttcaatcatg ccgtttttcg aacatgagta cgacagctta agcgatgacg
     2281 aaaaacgcat ctttattcgt ctgctggaat gtgacgatcc ggacctgttt aactggctga
     2341 tgaatcacgg taaaccggcc gatgcagaac tggaaatgat ggtccgactc atccagacac
     2401 ggaaccggga acgtggtcct gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg
     2461 ctttccttgc tgattcatgg gctggttgcc gctgttattt tactcatgcc ctggccactc
     2521 agttacaccc cgttatggat ggtgttactt tcgctggtgg tgtttgattg cgttcgcagc
     2581 cagcggcgta ttaatgctcg ccagggggaa attcgcttgt tgatggacgg gcgtttgcgt
     2641 tggcaagggc aggagtggag catcggcaaa gcaccgtgga tgattaagag cggcatgatg
     2701 ctgcgtttac gttctgatgg cggtaaacgg caacatttat ggctggcagc cgacagcatg
     2761 gacgaagctg aatggcggga tttacggcgg attttgttgc aacaagagac gcaaagataa
     2821 agatgacggc aaagcatatg cgctaagccg tcagtgagat caggcgtaat gctctgccat
     2881 ttcgttgagg atttgctcgc accagctctg aatacgctcg tcgctaaggt catactggtt
     2941 agtttcatcc agcgccagac ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct
     3001 ggtaaattca tatccttccg ttggccagta gccgacgaac ttcacgcctt tggtcgagag
     3061 tttgtcatgc agcataccga gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc
     3121 aagcccatac agcgcaacaa ttttaccttc aaggttcagg tcgtcgagct gatcccagac
     3181 ggcttcccag tcttcctgga tttcaccaaa atcccaggtc gggataccca gaatgagcac
     3241 atcgtactgc tccattaatt tcggggagtc gtccttgagg ttatgtaagg tcaccagttc
     3301 tgggccgata atatcgcgga ttttttctgc cgccatttcg gtgtaacagg tgctggaacc
     3361 gtaaaaaaga cccatattca taacgtaagc ttctcgattc tgctgtggct tttgcagcta
     3421 gtgtatcaga atcgctttag tcacggcaca ataaggcata atggatcaaa cgagtgacca
     3481 taaggggcgc aagtgaaaca ggatctggca cgcatcgagc agtttcttga tgctctgtgg
     3541 ctggaaaaaa atctggctga aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg
     3601 gtggagtggt tgcatcaccg cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag
     3661 gcattactgg cagaacggct ggagggcggg tataaagcca ccagctcagc gcgtttgctg
     3721 agtgcagtgc gccgattgtt ccagtatctt tatcgcgaaa agtttcgtga agacgatccc
     3781 agtgcgcatc tcgcttcacc gaaattgcca cagcgtttgc caaaagattt aagcgaagcg
     3841 caggtcgaac gtttattaca ggcaccatta attgatcagc cactggagct acgcgataaa
     3901 gccatgcttg aagtgttgta tgctaccggg ctgcgtgtct ctgaactggt cggactgaca
     3961 atgagtgata tcagcctgcg tcagggcgta gtgcgggtca ttggtaaagg caacaaagag
     4021 cgcctggtgc cgttaggtga agaggcggtt tactggctgg aaacgtatct cgagcatggg
     4081 cgaccgtggt tgttgaacgg tgtctctatt gatgtgttgt tccccagcca gcgtgcgcag
     4141 cagatgacgc gacagacctt ctggcaccgc attaaacatt atgccgtgct ggcgggtatc
     4201 gacagcgaaa agctgtcacc gcatgtgttg cgtcacgctt ttgccactca cttattaaat
     4261 catggtgcgg atttgcgcgt ggtgcagatg ctactgggcc acagcgatct ctccaccacg
     4321 caaatttata cgcatgtcgc taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg
     4381 cgggcgtgat gtctgaaaag aacgggaaga tttatgaaga aaggttttat gttgtttact
     4441 ttgttagcgg cgttttcagg ctttgctcag gctgatgacg cggcaattca acaaacgtta
     4501 gccaaaatgg gcatcaaaag cagcgatatt cagcccgcgc ctgtagctgg catgaagaca
     4561 gttctgacta acagcggcgt gttgtacatc accgatgatg gtaaacatat cattcagggg
     4621 ccaatgtatg acgttagtgg cacggctccg gtcaatgtca ccaataagat gctgttaaag
     4681 cagttgaatg cgcttgaaaa agagatgatc gtttataaag cgccgcagga aaaacacgtc
     4741 atcaccgtgt ttactgatat tacctgtggt tactgccaca aactgcatga gcaaatggca
     4801 gactacaacg cgctggggat caccgtgcgt tatcttgctt tcccgcgcca ggggctggac
     4861 agcgatgcag agaaagaaat gaaagctatc tggtgtgcga aagataaaaa caaagcgttt
     4921 gatgatgtga tggcaggtaa aagcgtcgca ccagccagct gcgacgtgga tattgccgac
     4981 cattacgcac ttggcgtcca gcttggcgtt agcggtactc cggcagttgt gctgagcaat
     5041 ggcacacttg ttccgggtta ccagccgccg aaagagatga aagaattcct cgacgaacac
     5101 caaaaaatga ccagcggtaa ataattcgcg tgaaacaaca gatacaactt cgtcgccgtg
     5161 cagtcgatga aacggcagac ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg
     5221 ccagccgggg cgtgcgcagt gcgcaagaac tggaacgcag tgttaaaggt atgttgccct
     5281 ggcagcaact gagcggcgtc gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag
     5341 gaacgcggat tattgtggtc ggcgattttg acgccgacgg cgccaccagc acggctctaa
     5401 gcgtgctggc gatgcgctcg cttggttgca gcaatatcga ctatctggta ccaaaccgtt
     5461 tcgaagacgg ttacggctta agcccggaag tagtcgatca ggcccatgcc cgtggcgcgc
     5521 agttaattgt cacggtggat aacggtattt cctcccatgc gggcgttgaa cacgctcgct
     5581 cgttgggcat tccggttatt gttaccgatc accatttgcc gggcgaaaca ttacccgcag
     5641 cggaagcgat cattaaccct aacttgcgcg actgtaattt cccgtcgaaa tcactggcag
     5701 gcgtgggtgt ggcgttttat ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct
     5761 ggtttgatga gcgcggcatc gcaattccta acctggcaga actgctggat ctggtagcgc
     5821 tggggacagt agcggacgtc gtgccgctgg acgctaataa tcgcattctg acctggcagg
     5881 ggatgagtcg catccgtgcc ggaaagtgcc gtccagggat taaagcgctg ctggaagtgg
     5941 caaatcgcga tgcacaaaaa ctagccgcca gcgatttagg ttttgcgctg ggaccgcgtc
     6001 tcaatgctgc cgggcgactg gatgatatgt ccgttggtgt ggcgctgttg ctgtgcgaca
     6061 acatcggcga agcgcgcgtg ctggcaaatg aactcgatgc gctaaaccag acgcgaaaag
     6121 agatcgaaca aggaatgcag gttgaagccc tgaccctgtg cgagaaactg gagcgcagcc
     6181 gtgacacgct acccggcggg ctggcaatgt atcaccccga atggcatcag ggcgttgtcg
     6241 gtattctggc ttcgcgtatc aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag
     6301 caggtgacgg tacgctgaaa ggttccggtc gctccattca ggggctgcat atgcgtgatg
     6361 cgctggagcg attagacaca ctctaccctg gcatgatgct gaagtttggc ggtcatgcga
     6421 tggcggcggg tttgtcgctg gaagaggata aattcgaact ctttcaacaa cggtttggcg
     6481 aactggttac tgagtggctg gacccttcgc tattgcaagg cgaagtggtg tcagacggtc
     6541 cgttaagtcc agccgaaatg accatggaag tggcgcagct gctgcgcgat gctggcccgt
     6601 gggggcagat gttcccggag ccgctgtttg acggtcattt ccgtctgctg caacagcggc
     6661 tggtgggcga acgtcatttg aaggtgatgg tcgaaccggt cggcggcggt ccactgctgg
     6721 atggtattgc ttttaatgtc gataccgccc tctggccgga taacggcgtg cgcgaagtgc
     6781 aactggctta caagctcgat atcaacgagt ttcgcggcaa ccgcagcctg caaattatca
     6841 tcgacaatat ctggccaatt tagcgtcatc ttctctataa aaaagagcgt ggattgggta
     6901 caatcccgct cttatcaccg cattttgact agctcaataa aagaaatcag accatgtttg
     6961 aaattaatcc ggtaaataat cgcattcagg acctcacgga acgctccgac gttcttaggg
     7021 ggtatctttg actacgacgc caagaaagag cgtctggaag aagtaaacgc cgagctggaa
     7081 cagccggatg tctggaacga acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc
     7141 ctcgaagccg ttgtcgacac cctcgaccaa atgaaacagg ggctggaaga tgtttctggt
     7201 ctgctggaac tggctgtaga agctgacgac gaagaaacct ttaacgaagc cgttgctgaa
     7261 ctcgacgccc tggaagaaaa actggcgcag cttgagttcc gccgtatgtt ctctggcgaa
     7321 tatgacagcg ccgactgcta cctcgatatt caggcggggt ctggcggtac ggaagcacag
     7381 gactgggcga gcatgcttga gcgtatgtat ctgcgctggg cagaatcgcg tggtttcaaa
     7441 actgaaatca tcgaagagtc ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa
     7501 atctccggcg attacgctta cggctggctg cgtacagaaa ctggcgttca ccgcctggtg
     7561 cgtaaaagcc cgtttgactc cggcggtcgt cgccacacgt cgttcagctc tgcgtttgtt
     7621 tatccggaag ttgatgatga tattgatatc gaaatcaacc cggcggatct gcgcattgac
     7681 gtttatcgcg cgtccggcgc gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt
     7741 attacccaca tcccgaccgg gatcgtgacc cagtgccaga acgaccgttc ccagcacaag
     7801 aacaaagatc aggccatgaa gcagatgaaa gcgaagcttt atgaactgga gatgcagaag
     7861 aaaaatgccg agaaacaggc gatggaagat aacaaatctg acatcggctg gggcagccag
     7921 attcgttctt atgtccttga tgactcccgc attaaagatc tgcgcaccgg ggtagaaacc
     7981 cgcaacacgc aggccgtgct ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa
     8041 gcagggttat gaggaaccaa catgtctgaa caacacgcac agggcgctga cgcggtagtc
     8101 gatcttaaca atgaactgaa aacgcgtcgt gagaagctgg cgaacctgcg tgagcaggga
     8161 attgccttcc cgaacgattt ccgtcgcgat catacctctg accaattgca cgcagaattc
     8221 gacggtaaag agaacgaaga actggaagcg ctgaacatcg aagtcgccgt tgctggccgc
     8281 atgatgaccc gccgtattat gggtaaagcc tcttttgtta ccctgcagga cgttggcggt
     8341 cgtattcagc tgtacgttgc ccgtgacgat cttccggaag gcgtttacaa cgagcagttc
     8401 aaaaaatggg atctcggcga catcctcggc gcgaaaggta aactgttcaa aaccaaaacc
     8461 ggcgaactgt ctatccactg caccgagctg cgtctgctga ccaaagcact gcgtccgctg
     8521 ccggacaaat tccacggctt gcaggatcag gaagcgcgct atcgtcagcg ttatctggat
     8581 ctcatctcta acgatgaatc ccgcaacacc tttaaagtgc gttcgcagat cctctctggc
     8641 attcgccagt tcatggtaaa tcgcggcttt atggaagttg aaacgccgat gatgcaggtg
     8701 atcccaggcg gtgcggctgc gcgtccgttt atcacccatc ataacgcgct ggatcttgac
     8761 atgtacctgc gtatcgcgcc ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag
     8821 cgtgtattcg aaatcaaccg taacttccgt aacgaaggta tttccgtacg tcataaccca
     8881 gagttcacca tgatggaact ctatatggct tacgcagatt acaaagatct gatcgagctg
     8941 accgaatcgc tgttccgtac tctggcacag gatattctcg gtaagacgga agtgacctac
     9001 ggcgacgtga cgctggactt cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc
     9061 aagaaatatc gcccggaaac cgacatggcg gatctggaca acttcgactc tgcaaaagcg
     9121 attgctgaat ctatcggcat ccacgttgag aagagctggg gtctgggccg tatcgttacc
     9181 gagatcttcg aagaagtggc agaagcacat ctgattcagc cgaccttcat tactgaatat
     9241 ccggcagaag tttctccgct ggcgcgtcgt aacgacatta acccggaaat cacagaccgc
     9301 tttgagttct tcatcggtgg tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg
     9361 gaagatcagg cgcaacgctt cctggatcag gttgccgcga aagacgcagg tgacgacgaa
     9421 gcgatgttct acgatgaaga ttacgttacc gcactggaac atggcttacc gccgacagca
     9481 ggtctgggga ttggcatcga ccgtatggta atgctgttca ccaacagcca taccatccgc
     9541 gacgttattc tgttcccggc gatgcgtccg gtaaaataag cattacgtta tgctcacaac
     9601 cccggcaaat gtcggggttt tttaattgtg ctgcgcgaaa gtagacaaca attttcttgc
     9661 ttcactattg gctgcctgca tcaccatcca cggactgaac gcccacggcg tggcatcaat
     9721 accgtgtaat acatcagcta aatcacacca ttgataatcc atcacttcat catcgttgat
     9781 ctgtaacgca ctggtggtgc gtgcggcaaa taccggacac acttcatttt ccacaatgcc
     9841 actcggatcg gtggcgcggt agcgaaagtc aggatagata gattcaggag gcgtaatttc
     9901 cacgccaagc tcataacggc aacggcggat caccgcgtct tcgctgcttt ctcccagttg
     9961 tgggtgccca caaaccgagt tagtccacac gccaggccac gcttttttgc tcagtgcgcg
    10021 gcgggtaact aataattgcc ccttggcatt aaacagccaa ctggagaacg cgagatgtaa
    10081 gagggtgtct gccgtgtgtg cggcatactt ttccagcgta ccagtgggaa ctccctgtgc
    10141 attcaataaa atgacgtgtt ccgtttgcat aatttctcac atgtaattct gatcactcat
    10201 cattatagat aattgaagag tgaaatgatt aaccaagtga gcgtttatcg acaaccgccc
    10261 gttttgagcg gatgccgaca ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag
    10321 cgactttcaa tggccattgc aacacagtca gaaatgacag gaatagagag agtaaaaagc
    10381 aacagcagac tgaatatagc gcgcattaaa agattcatat tattctccat gctcattttt
    10441 cttcagcttg cgcatcagaa gccacatacg tactgtcctg acttttaaat ctttatcaga
    10501 gacggattcc ttcaccaggt cagcatgatg ctgatagcca ttaaagaaga cgttcagcac
    10561 aacggcgcta agggttgcga gcataatgcc gctatgtagc agcggttgca gtacggccgg
    10621 taacttagaa aagaaatcgt gagagagcgt cggagtcatg ccaacgccga gactgatcgc
    10681 cacaatatag aggttgtaac ggttggtggt gtagttacag cgcgacagaa ttcgaatccc
    10741 tgtcgccagt accatgccga acatcaccag cccagcaccg cccagcacaa attgcggaat
    10801 ggaggcgacc agcaccgcca ttttggacac catgccaaac aggattaata taattcccga
    10861 agaaatacac acccagcggc tatgaacgcg cgtcacgcta accaggccaa cgttttgtga
    10921 aaatgacgtg tgggggaagc tgttaaacgt tccgcctatc attgtcccta cgccatcgac
    10981 acgcagcccg cgaataatat cgtgtgaaga gagtttacga ccgactattt cacccagtgc
    11041 taggaacatc cccatcgact cgataaacac gatgattaac acggcagtca tggtcagaat
    11101 ggaaacggga tcgaaaatcg gcataccaaa tgacatcggc gtgacaatcg caaaccatga
    11161 agcatcatgt agcccggata aattgacttc attcatcatc cacgaaagta aaaagccaaa
    11221 tacaatcccc agtaatacgg cgacgttgga cataaatcct ttcgcatagc gagtaatgag
    11281 caagataaaa attaagacag caaaggagat acctaaataa acgggattac catattgcgg
    11341 attcccttta cctccggcgg cccagtcaat acccacctga atgatgctaa gcccgataga
    11401 agtaataacc acaccggtaa ccagtggcgg gaataaaggc atcaagcgac cgataagtgg
    11461 cgctaataat gtggtgataa aacccgcggc gatagtggca ccaaatatcc ccagcaggcc
    11521 gatatccggg ttcatcccaa tggctatcat tggtgttaca gcagcaaagg tcaccgacat
    11581 aatcaccggc aggcggatcc ccataaagcg gccgataccg atacattgca ataatgtgac
    11641 gatcccgcag caaaagagat ccgagctaat gagcatcgca atagcttctt ttgagaggcc
    11701 cagtcggtca ccaatcataa gaggaacagc gactgcacct gcgtacatga caaggacgtg
    11761 ttgcagaccg agtatgatca gctttcctgg tgataatatg cggtcaacct catcagttgg
    11821 gcggtcttgc ccagaagatg agggaagttg ggaatctatg gcgctcatca gtgtttctcc
    11881 tttcgcccgc cagagtaaaa agcaaccgtt tacgttaatg aaactgtcta aacacatgct
    11941 cgtggagtgc cagtttttgc gaggaagggg agttagagac aggaattacg gaggcaatca
    12001 cataatcgtg gttaaaccac gaaaatgaga agagaacctg acaatgaaat tatctgtaat
    12061 tttctcatat tgatatttca ttaatcattc tggcatgtcg cactctcgca tttaatcgtt
    12121 tttatctgga tagcgctctt ttggtcggca atgttgaaat aatgaaagcg gcatagattg
    12181 aggggcaata atttgaactc agtcgcaaaa ttataattaa aacggtcatg ctacctaata
    12241 tatgcccgtt tattgcatcg cgaaatttga gaagacataa ctcattaaat gaaagggatg
    12301 caaatgaata agtttatcgc tgctgaagct gcggaatgta taggctgtca tgcttgtgaa
    12361 attgcctgtg cggtggcaca caatcaagaa aactggccgc tgagtcacag tgactttcga
    12421 ccgcgtatcc acgttgtagg gaaaggccag gctgcgaatc cggtggcctg ccatcactgc
    12481 aacaatgccc cttgcgttac ggcttgtccg gttaatgctc tgactttcca gtccgatagc
    12541 gtacaactgg acgagcaaaa atgtattggc tgtaaaagat gcgcaatcgc ttgccccttt
    12601 ggcgtcgttg agatggtcga tacgattgcg cagaaatgcg acctttgtaa ccagcgcagt
    12661 tccggcacgc aagcctgtat tgaagtctgc ccaacacagg cgttacgact gatggacgat
    12721 aaagggttac agcagataaa ggtggcccgc cagcgcaaaa cggcagcagg aaaagcgtca
    12781 tcagacgctc agccatctcg cagtgcagcg ttgctccccg ttaactcgcg taaaggcgca
    12841 gataaaattt cagcgagtga acggaaaacc cactttggcg aaatatattg cgggctggat
    12901 ccacaacaag cgacttatga gagtgaccgc tgtgtttatt gtgccgaaaa agctaactgc
    12961 aactggcatt gcccgctgca taacgctatt ccggattaca tccgtctggt acaggaagga
    13021 aagattattg aagcggcaga actttgccac cagaccagtt ccttaccaga aatttgcggc
    13081 agggtatgtc cacaggaccg tctttgtgaa ggcgcatgta ctttgaaaga tcactcgggc
    13141 gcagtctcta tcggtaatct ggaacgctac atcaccgata ccgcgctggc gatgggctgg
    13201 cgtcccgatg tcagcaaagt tgttccccgt atcgaaaaag tggcggtgat tggcgctggg
    13261 ccagcagggt tagggtgtgc tgatattctg gcgcgcgcgg gagttcaggt tgatgtcttt
    13321 gatcgccatc cagaaattgg cggtatgctg acttttggca ttcctccttt caaactcgat
    13381 aaaacggtat taagccagcg gcgagagata ttcaccgcaa tgggaatcga ctttcatctt
    13441 aactgtgaaa ttggccgcga tattaccttt agcgatttaa cttctgaata tgatgcagtt
    13501 ttcatcggcg tggggactta cgggatgatg cgagcagatc tgccgcatga agatgcgccc
    13561 ggtgtcattc aggctctacc gttcctgact gcccataccc gccagctcat gggattgccg
    13621 gagtctgaag agtatccgct gacggacgtg gaaggtaagc gagtcgtggt attgggcggt
    13681 ggcgatacga caatggattg tttgcggact tccatccgcc tcaatgccgc cagcgtgacc
    13741 tgcgcgtatc gtcgtgatga agtcagtatg ccgggctcgc gcaaagaggt ggttaatgcg
    13801 cgcgaggaag gtgtcgagtt tcagttcaat gttcaaccgc aatatatcgc ttgtgacgaa
    13861 gatgggcgtt taactgcggt gggcctgatt cgtaccgcca tgggtgagcc ggggccggat
    13921 ggtcgccgtc gtcctcgtcc ggtagcgggt tcagagtttg aattgcccgc cgatgttctg
    13981 attatggcct ttggtttcca ggcgcatgcc atgccgtggt tgcagggcag cggaattaaa
    14041 ctcgataaat ggggcctgat tcaaaccggt gacgtcgggt atttacctac ccagacgcat
    14101 ctgaaaaaag tctttgctgg tggtgatgca gttcatggcg cggatctggt tgtcactgca
    14161 atggccgcag gaaggcaggc ggcgcgcgat atgttaactc tgtttgatac gaaggcatcg
    14221 tgatgaaatc gttaattatc gttaatccgg ctgactgtat tggctgccgt acttgtgaag
    14281 tagcctgtgt ggtcgctcac ccttcagaac aggagttgaa tgccgatgtc tttttacccc
    14341 ggctgaaggt acagcgactg gacagcatca gtgcgccggt gatgtgccat cagtgtgaaa
    14401 acgccccttg tgttggcgct tgccccgtgg gggcgctgac gatgggcgag caggtggtgc
    14461 agaccaattc tgcccgttgt attggctgtc agagttgcgt cagtgcgtgc ccgtttggaa
    14521 tgatcaccat tcagtcgttg ccgggggata cccgacaaca aattgtgaaa tgcgatctct
    14581 gtgaacagcg ggaagagggg ccagcctgcg ttgaatcttg cccaacacag gcgctgcagt
    14641 tgcttactga aagagaactc aggcgagtcc gccagcagcg tattgttgcc agcggtgaga
    14701 atcctctgtg aaagaaatct ccgcgctgaa aggcgcggag aatctattag attgcccaac
    14761 cacccgcgta aaatgcgacc agtgcggcag taatgatcac cgtaccaata ttcagctttt
    14821 gccattcgcg tgcaaagacg cggcctacga ccagtgtcac aaagcccagc ataataccgg
    14881 taacgatatt acaagtcaga acgatgaaca cggcacacac caggccagcc atagcgtcaa
    14941 taaaatcatt gaaatccagc ttcgagacgt tacttaacat cagcaaacct atgtacatca
    15001 gagcgggtgc agtggcgtaa ccagggatca aaaatgacag cggtgataag aacagaatca
    15061 gcaggaataa cgcccccact acggttgcgg ttaaccctgt tttacccccg gcggcggttc
    15121 ctgccgctga ttcgatataa accgctgcgg gcgctgcgcc caccaggccg gagaatattg
    15181 aacttactga gtcactggtc agggctttgc cgccgttgat gatctggttg tctttatcca
    15241 acaaattcgc ctgaccggcg acggcacgga tggtgccagt agcgtcgaac actgcggtca
    15301 tcaccaatgc cagtacactc ggaagtacgg ttggctggag tgcgcccata atatcgaggc
    15361 tgaaaatcag agacttacca tcttcgccag tcagacttgg catcgccacc agaccgtggt
    15421 atttcaccgc tgggtcaaag attaagccga taatcgaaat tgcgataatc accaacaaga
    15481 tcccgccggg tacgcggcac ttctccagac cgaagatgac cgccagcccc agcaagctca
    15541 tcatcaccgg gaaggaggta aacgcaccga gcgccactgg caagccttca atcgggtttt
    15601 tgataaccat acccacaccg ttagcagcaa tcagcagcag aaacaggccg ataccgatac
    15661 ctgtaccgtg agcgataccc atcggtaaat tacgtaagat ccaggtacgc acaccggtta
    15721 cggaaatagc ggtgaagatg acgcccatca gaaagaccgc gcccagtgcg acaggaacgc
    15781 taatttgttg cccgagtacc agactgaatg cggtaaatgc cgtcaaggaa atcgcacaac
    15841 caatcgccat tggcaaattg gcccacaacc ccatcagcaa cgagccgaag cccgcgacca
    15901 gacaggtggc aacaaacaca gctgcgggag gaaaacctgc tttgcccagc attcccggaa
    15961 cgacgataac ggaataaacc atggccagaa aggtcgttaa gccagccagt acttcctgac
    16021 gaacggtact gccacgagcg gtaattttaa aataattatc cagcgcgccc tgtggctttg
    16081 gtgcgtccgg tgtttgtagg atgtctccag acataatgat gtcctcacga ggatgttaaa
    16141 gttttattat tagttgcgtt cgtacaccag acgaccatca acgtaggtgc ggtagatcga
    16201 acggtcatcg cccaacgtca tcatcacgaa caatttgtcg actaaagaaa cagagttgtc
    16261 atagcgcagc tgctgtagcg gagtggcggt gggttccatc accacgaaat cagcctcttt
    16321 gccaggtaaa aagttgccaa tcaaatcgtc aaggcccaga gatttcgctc cgccgagcgt
    16381 ggccaggtaa aacgcttcat atgccgagag gcgatagcct tgtaattgca atactttgta
    16441 ggcttcgttc agcgtttgca gcatgttgaa agtggttccg gcaccgatat ccgttcccat
    16501 gcccacttta actttcttct gccatgcttt tttcaagttg aataagccgc tgccgaggta
    16561 aaggttggag gtcggacaga aagcaatgct ggatttggtt tcgctgagac gatcccactc
    16621 tttttcttcg agatggacgc agtgagcaaa gacacagttt ttaccggtca ggccgtactg
    16681 atggtaaaca tccagataac catcatggtc aggataaagc gatttcaccc aggcaatttc
    16741 atctttgttt tcacagagat gggtatgtac ccacgtatcc ggatattctt ctttcaggcg
    16801 ttgcgccatc gccatctgtt caggagatga ggtcggggcg aagcgtggcg taatcgcata
    16861 tagcagacga ccatttttgt gccagcgttc aatcagttct ttgctttggt gatagctgct
    16921 ttcggcagtg tcgagcagat aatccggtgc gttgcggtcc atcatcacct taccggcaat
    16981 catacgcata ttgatatgac tggcggcttc aaacagcgca tcaacagatt gcggatgaac
    17041 agtgccaaac accagcgccg tggtggttcc gttacgtaaa agctgcttga tgaagaatgc
    17101 cgacatttcg cgggcgtact ctaaatcctc ataacgacgt tcagtaggga aggtgtgttt
    17161 attcaaccac tccagcaatt gttcaccata ggcccccacc atttcacttt gcggataatg
    17221 gatatgtgta tcgacaaagc ccggtactat cagtttgccg cgatagtcgc gcacgcgaat
    17281 agtgtcagga atttgatgct ttccgtcttc ccattcgcca aaccattcca cttttccctg
    17341 tttaatgagt aataaaccat cctcaataaa ccgcagcgca gaggcaatct cttccgggtt
    17401 atcgaccgta cgggtgacat caataaaact gcctcgtacc gctttcaacg tgtgttctcc
    17461 tgacatcatt gactccttta ctgttaatcc atctcttccg ctgaggtaat accaggcaga
    17521 acgttttcct gtcggtagcc accagggagg ataatattta ataaaatcgc agttaacccg
    17581 ccagcacaaa tagggttttc aactagtaca taaatagagg ctggcaatat tttaaaaatt
    17641 tcgggatcgt aggagacgcc aagtcctaaa cctaaagaag tggcgacaat aagtgtttct
    17701 cggcgcttta aaccgttggt gatgattatg cgaatcccag cgatggcaat catggaaaac
    17761 atcaacgtca ttgcgcctcc cagaactgcc gagggaatgg tcgtgaagaa gccgccaatc
    17821 atcggaaaca aaccgaggat aaccagcatt accgcgatgg ttcgcccgac ataacgtgaa
    17881 gcgacgccag tcatctgaat aaccccatta ttttgcgcaa acgtggttaa gggtaatgac
    17941 ccgacagcgg aggcgataac agaaaccaga ccgtctgcca gcacgccgcc tttcagtcgg
    18001 gactgatact cttctccctg aatgggtcgg cgggaaacca ttgccgtggc ggtgatatcg
    18061 ccgacagctt ccagcacgct aagcagataa atcgtgccaa ccaccaggaa ctgatggaag
    18121 ctaaaactaa agccgtattt gaacggatgc gggatggtga ttaacggcaa attgcgcata
    18181 ctgctgaaat ccaccatgcc caggcataac gatgcgatat agccgacaca tagcccaatg
    18241 gcgatccctc ccatgcgtag caacggactg cgacagcagt taaagccgat caccacaatt
    18301 aaaaccaata aaccaacgcc gagatgttcg taattgccga atgtaccgct gcttttggct
    18361 gcaaatcctc caccaaaatc gataatgccg actttaatca ggcttaagcc gatcatcagt
    18421 accacaatac cgctgacggt aggcgtaata acccgacgta aatagggcaa gataaatgaa
    18481 gatccgacaa ccagaaatgc gccaacgaag gagacgccga gaagcgacga catgattaac
    18541 tcttcgtgaa aaccgtcgct tttcatgctg ctgcccagcg caatcatgac cgtaacaaat
    18601 gaaaaattga ctgactgaat tgagagtagg ccagaaccga cgatgccgta gcggtttact
    18661 tgtaaccagg taccaatacc agaggcgatc atcgccatag aaacaagata ggcagttgtt
    18721 tcagcggaaa gctgtaaggc cgcccccacg attaacgcgg gggttaccat cggaacgaaa
    18781 attgccaaca gatgggtaat ggcaccaacg agagcctgat gaaagggagg gcgatcctcc
    18841 agttcaaata taaggtcaga acctgcattg tttatatcag acatcccttc actcctttta
    18901 aa
//
LOCUS       DAZGVQ010000098        18381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000098 DAZGVQ010000000
VERSION     DAZGVQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.099"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            87..782
                     /gene="yafL"
                     /locus_tag="ACHLAG_003298"
     CDS             87..782
                     /gene="yafL"
                     /locus_tag="ACHLAG_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0656468.1"
                     /translation="MPFSSFASATTSHITFSYAARQRMQNRARLLKQYQTHLKKQASY
                     IVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQALAENNHFLSSDHLHNITE
                     VAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILEAKLPRTANEMYHYRRATI
                     VANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGEIIRVSQLAEPFWQDHF
                     LGARRILTEETIL"
     gene            complement(839..1195)
                     /locus_tag="ACHLAG_003299"
     CDS             complement(839..1195)
                     /locus_tag="ACHLAG_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP0656469.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(1188..1466)
                     /locus_tag="ACHLAG_003300"
     CDS             complement(1188..1466)
                     /locus_tag="ACHLAG_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFP0656470.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            complement(1571..3283)
                     /locus_tag="ACHLAG_003301"
                     /pseudo
     CDS             complement(1571..3283)
                     /locus_tag="ACHLAG_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            3255..4040
                     /gene="lafU"
                     /locus_tag="ACHLAG_003302"
     CDS             3255..4040
                     /gene="lafU"
                     /locus_tag="ACHLAG_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFP0656471.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFSGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            4111..5166
                     /gene="dinB"
                     /locus_tag="ACHLAG_003303"
     CDS             4111..5166
                     /gene="dinB"
                     /locus_tag="ACHLAG_003303"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP0656472.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELHLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            5163..5615
                     /gene="yafP"
                     /locus_tag="ACHLAG_003304"
     CDS             5163..5615
                     /gene="yafP"
                     /locus_tag="ACHLAG_003304"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0656473.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            5861..6970
                     /locus_tag="ACHLAG_003305"
     CDS             5861..6970
                     /locus_tag="ACHLAG_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFP0656474.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALAFACINRQMIALRIMQQVK
                     ATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDYSWIVQPVANE
                     KTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            6967..7581
                     /gene="prfH"
                     /locus_tag="ACHLAG_003306"
     CDS             6967..7581
                     /gene="prfH"
                     /locus_tag="ACHLAG_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFP0656475.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(7638..9095)
                     /gene="pepD"
                     /locus_tag="ACHLAG_003307"
     CDS             complement(7638..9095)
                     /gene="pepD"
                     /locus_tag="ACHLAG_003307"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP0656476.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            9356..9814
                     /gene="gpt"
                     /locus_tag="ACHLAG_003308"
     CDS             9356..9814
                     /gene="gpt"
                     /locus_tag="ACHLAG_003308"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP0656477.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            9906..11150
                     /gene="frsA"
                     /locus_tag="ACHLAG_003309"
     CDS             9906..11150
                     /gene="frsA"
                     /locus_tag="ACHLAG_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP0656478.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKALQEITGWIEKRLC"
     gene            11208..11609
                     /gene="crl"
                     /locus_tag="ACHLAG_003310"
     CDS             11208..11609
                     /gene="crl"
                     /locus_tag="ACHLAG_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP0656479.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(11648..12703)
                     /gene="phoE"
                     /locus_tag="ACHLAG_003311"
     CDS             complement(11648..12703)
                     /gene="phoE"
                     /locus_tag="ACHLAG_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFP0656480.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            12991..14094
                     /gene="proB"
                     /locus_tag="ACHLAG_003312"
     CDS             12991..14094
                     /gene="proB"
                     /locus_tag="ACHLAG_003312"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP0656481.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            14106..15359
                     /gene="proA"
                     /locus_tag="ACHLAG_003313"
     CDS             14106..15359
                     /gene="proA"
                     /locus_tag="ACHLAG_003313"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP0656482.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQTGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            15474..15549
                     /locus_tag="ACHLAG_003314"
     tRNA            15474..15549
                     /locus_tag="ACHLAG_003314"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15507..15509,aa:Thr,seq:cgt)
     gene            complement(15676..16014)
                     /locus_tag="ACHLAG_003315"
                     /pseudo
     CDS             complement(15676..16014)
                     /locus_tag="ACHLAG_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(16065..17021)
                     /gene="paoD"
                     /locus_tag="ACHLAG_003316"
     CDS             complement(16065..17021)
                     /gene="paoD"
                     /locus_tag="ACHLAG_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFP0656483.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIHGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPALQAAREAKPFYLGALGSHRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEIASVRLHHEEDSCLPPSS"
     gene            complement(17031..>18381)
                     /gene="paoC"
                     /locus_tag="ACHLAG_003317"
     CDS             complement(17031..>18381)
                     /gene="paoC"
                     /locus_tag="ACHLAG_003317"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFP0656484.1"
                     /translation="ATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLY
                     AGANRHTGLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDT
                     QVDPADPTRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNL
                     LEKSGARVHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFP
                     VSAGSGGQWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHE
                     ATAGGRLTAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRIL
                     NPKTARSQVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFL
                     DDTDPISSPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
BASE COUNT         4345 a         4779 c         4864 g         4393 t
ORIGIN      
        1 caacatcaat gcatgatcag ccattattga gtatgtcctt gccgtcgatt ccctcttttg
       61 tgttgtcggg attactgttg ctttgcttgc cgttttcttc atttgccagc gccaccacat
      121 cacatatcac tttcagctac gccgcccgcc agcggatgca aaatcgtgcg cgtttattaa
      181 aacagtacca aacccatctg aaaaaacagg ccagctatat tgtggaaggc aatgccgaaa
      241 gcagaagggc gctacgccag cacaaccgcg aacagataaa acagcatcca gaatggtttc
      301 ctgcgccgct caaggcgagt gacagacgct ggcaggcgct ggcggaaaac aaccatttct
      361 taagcagcga ccatctgcat aacatcaccg aagtggcgat tcaccgtctg gagcaacagc
      421 ttggcaagcc ttacgtctgg ggcggtacgc ggcccgatca aggcttcgac tgtagcgggt
      481 tagtttttta tgcctacaac aagatccttg aggcgaaact cccacgcacg gctaatgaga
      541 tgtatcacta tcgccgggca accattgtgg cgaataacga cctgcgccgg ggagatttgc
      601 tatttttcca tatccacagc cgcgagattg ccgaccatat gggggtgtat ctgggcgacg
      661 gtcagtttat tgagtcaccg cgcaccgggg aaatcattcg ggtaagtcag ttagctgaac
      721 ctttctggca ggatcacttt ttaggcgcgc ggcggatttt gacggaggag acgattttgt
      781 aaatgggtaa tattgacgaa ctggcaaaag gtcggatgcc acgcggcatc cgacctgttt
      841 acatctgggc tgaaaactca cgttctaaga catcaacaat gagctggttt tttgaaatgg
      901 aatgttgtct ggcgacgatg gtcagccgcg aatcaatgtg atgaggaaca cgaagcgtca
      961 ggcgtttttg ctcttcttct tcacgaaaag gggcaatacc gtcctcttca caatccgcca
     1021 tccactcatc cagcgcgctt tcgccttcac gatataatgc ctcaatactt gtggctacaa
     1081 agtcacaata accgtttaca ttgaggaact tgccgcgaaa tgcgttgata tcggcttcaa
     1141 atgttactgc ggctggttga cctttaattg tgataatgtt cgatgcttta ttcataatct
     1201 acccctacac agttaacaaa ccactcctta agacctgcta ctgcgccttt atccatcaca
     1261 ttttttggat gtggctcatg tgtgctgaat ttggtcgttc caatctgaaa ttttcgtctg
     1321 gagccgttgt tattttttat tgttccaccc acagctttaa tgagtgcaac aacatcatcc
     1381 catttaattc cagattttgg cggctccgtc attacatcgt cgaatgtttt tctatgacga
     1441 gaacctagcg cgctgacctt aatcatacta tcaatccatg acgtcattgt atggtgtcat
     1501 cgtagtctct ttgcagtgtg acgtcaatta atggtgtcag gtgctgcaaa gcatggaaaa
     1561 gaatccccca tcaactcaac gcccccataa tcttcaactc cagctcatcc ggcacttcgt
     1621 tatacgacag cacatgcagc cccggcgcaa acaaccttgc ataacgcgcc agcaaagggc
     1681 gcagctgcgg tggcaccagc agcaccgggt ctttccccgc cgctttcatc tgctctttca
     1741 cctgtggcat cgtactctgg aactggttga gcatattcgg gtccactggc acgctgtcga
     1801 gcatcacttt accgccctgt tgcgcctgat tcactaggtt agtcagcaga ttttccagct
     1861 cattattcag ggtatacacc gtcagctcct gcttgcgaac gaacggatgg gtaatgctgc
     1921 gccgcagcgc caggcgcaca tcggctgcca gcagaatatg atctttggtc accgcgctac
     1981 tggcgaccag cacggtggcg atggtgacga tatcgcgcag ggaaacgcct tcggtcagca
     2041 gcgcccggta cactttcagc aactggctgt aattgagcac cgcgcttaaa tcctccgcca
     2101 ggcgcggtgc catcgacgcc aaacggttat gcaactgcgt aatatcgtca tagttaaata
     2161 aatcaggaat atagctgcgc acaatcttat ttacatgggt agcaatcacg ctggcgctgt
     2221 cgatcacctg ataccccata ttcagcgcct tcgctttctg cgcaggctgg atccaggtca
     2281 ccggcatccc gtacgccggg tcgttcccca gcacgccgtc tatctcgccg taggtttcgc
     2341 tggagggcag cgccatcagt ttatccgtcg gaatatccgc ttcatcagcc ttaatgccgt
     2401 tgatgaaaat agcgtactgg ctgggcttaa ggcggaagtt ttcccgaatg cggatctccg
     2461 gcagcagcac gccgttgccg tcggatatca cctgtcgcac gccgcgaata cgctgggtga
     2521 gcgggttgcc ctgggctttg tccaccagcg ccaccagctt gtagccgagg cttaagctga
     2581 tgggctcgat cagcggaatg gtttcccaac tgacctgttg ctcgctggtt tcggtgatgg
     2641 tgcgggtcag cgtttcgaga cttttctctt ccgcttccgc cgcctgcggg cgtttgctca
     2701 tccgccagcc ggtaaagcca agcagggcgc tgaacagcaa aaacggcaga tgtggcattc
     2761 caggcactac cgccagtacg aacataattc cggtagcggt gtagagcacc gatgggcttg
     2821 ccagcaactg gtgacgcacg tcatgggcga tatcgccgtt gtcgctgacg cgggtgacga
     2881 taatcgccgc cgcggtggag agtagcaggg aagggatttg cgccaccagc ccgtcgccga
     2941 tggtcatcag cacatactgc tggaaggcgg cgtcggcgct caggttgtat ttgaagatcc
     3001 cgatacagac gccgccgatc aggttgatcg ccagaatcat catcccggcg atagcatccc
     3061 cgcgcacaaa tttcgatgcc ccgtccatcg cgccgtagaa atcggcctcg ctggcaacat
     3121 ctttacgtcg ggtttgtgcc tgcgcctgat tgatcaaccc ggcattaaga tcggcgtcaa
     3181 tcgccatctg cttaccgggc atcgcatcca gggtaaagcg ggctgaaacc tcggaaatac
     3241 gctcggcccc tttggtgacg acaataaagt tgattgtcaa cagcgtcagt aaatccgagc
     3301 gtgaaagcat tatcgccgcg ctgcacgggc agtcgatttt cagtggcggc ggattgtcgc
     3361 cgttaaataa aatcagcccg tctcatccgc caaaacctgc caccgtggcg gtgccggaag
     3421 agacggagaa aaaagcgcga gacgtcaacg agaaaacagc gctgctgaag aagaaatccg
     3481 ccaccgagct tggcgagctg gcaaccagta tcaacaccat tgcccgtgat gcgcatatgg
     3541 aagcgaatct ggagatggag attgttcccc agggattgcg cgtgctgatt aaagatgacc
     3601 agaaccgcaa tatgttcgaa cgcggcagcg cgcagattat gccgttcttt aaaactttgc
     3661 tggtggagct cgcgccagtg ttcgactcgc tcgataacaa aattattatt accgggcata
     3721 ccgatgcgat ggcctacaaa aacaatatct acaacaactg gaacctttcg ggcgatcgcg
     3781 cgctttcagc tcgtcgggtg ctggaagagg ccggaatgcc ggaagataaa gtgatgcagg
     3841 taagcgcaat ggcggaccag atgctgctgg atgccaaaaa tccgcaaagc gcgggcaatc
     3901 ggcgtattga gattatggtg ctgaccaaaa gtgcgtccga tacgctgtat caatactttg
     3961 gtcagcatgg ggataaagtg gtgcagccgc tggtgcaaaa gctggataag cagcaggtgc
     4021 tttcgcagcg aatgcgctaa atgctgaatc tttacgcatt tctcaaaccc tgaaatcact
     4081 gtatacttta ccagtattga gaggtgagca atgcgtaaaa tcattcatgt ggatatggac
     4141 tgctttttcg ccgcagtgga gatgcgcgac aatcccgccc tgcgcgatat ccctattgct
     4201 attggcggca gccgcgaacg tcggggggtg atcagcaccg ccaattatcc cgcgcgtaaa
     4261 tttggcgtac gtagcgctat gcctactggg atggcgctca aattatgccc acatctcacc
     4321 ttgcttccgg ggcgctttga cgcctacaaa gaagcctcaa atcatatccg tgaaatcttc
     4381 tcgcgctaca cctcgcgcat tgaaccgttg tcactggatg aggcttatct cgatgtcacc
     4441 gatagcgtcc attgccacgg ttctgcgacc ctcatcgccc aggaaatccg ccagacgatt
     4501 ttcaacgagc tgcacctgac ggcgtctgcg ggcgttgcgc cggtcaagtt tctcgccaaa
     4561 atcgcctccg acatgaataa acccaacggc cagtttgtga ttactccggc agaagttccg
     4621 gcatttctgc aaaccttacc attggcaaaa atccccggcg tcggcaaagt ctcggcggca
     4681 aaactggaag cgatggggct gcggacctgc ggtgatgtgc aaaagtgtga tctggtgatt
     4741 ctgcttaaac gctttggcaa atttggccgc attttgtggg agcgtagtca ggggattgac
     4801 gagcgcgacg ttaacagcga acggttgcga aaatccgtcg gcgtggaacg cacgatggcg
     4861 gaagatatcc accactggtc tgaatgtgaa gcgattatcg agcggctgta tccggaactt
     4921 gaacgccgtc tggcaaaggt aaaacctgat ttactgattg cccgccaggg ggtgaaatta
     4981 aagttcgacg attttcagca aaccactcag gagcacgtct ggccgcggct gaataaagct
     5041 gacttaatcg ccaccgcgcg taaaacctgg gatgaacgcc gcggcgggcg cggtgtgcga
     5101 ctggtggggc tgcatgtgac gttgcttgac ccgcaaatgg aaagacaact ggtgctggga
     5161 ttatgaataa catacaaata agagactatc agcctggcga ttttcagcaa ctatgcgcta
     5221 ttttcattag agcggttacg atgactgcca gtcagcatta ttcaccacaa caaattgccg
     5281 cctgggcgca gattgacgaa tctcgctgga aggagaaact cgcgaaatca caagtgcggg
     5341 ttgcagtcat taatgcaaaa ctggtcggct ttattacctg cgttgaacac tatatcgata
     5401 tgttatttgt tgaccctgaa tacacccgcc gtggggttgc cagcgctttg ttaaaacctt
     5461 tgattaagtc tgaatccgaa cttacggtgg acgcaagcat aaccgcaaaa cccttttttg
     5521 aacgttatgg ttttcagaca gttaagcagc aacgcgttga atgtcgggga gagtggttta
     5581 ttaattttta tatgcgttat aaacagcaac attaaatcca gcttgcaatg aaaataacgc
     5641 aagcctagta tgtgcgcgtt ttcttattcc acaaactgca aggaggtaaa tcatgacaaa
     5701 tcctttatca atgactcttt gcagaccttt ccaggattaa ttcttttttt cttgccctgg
     5761 attcgtctgc catttcctga ttttttaatt gttactctca ataattcaag tcgcaacaca
     5821 tgcaacttga agtatgacga gtctatttac ggaatggtat atgggcaatt atattcgtcc
     5881 cttatccgat gcggtattta ccatcgcatc tgatgacctg tggatcgaga gtttagcgat
     5941 ccaacaatta cacaccacgg caaatttacc caacatgcag cgcgtagtgg ggatgccaga
     6001 tttacatccc ggacgcggct atccgattgg cgcagcgttc ttctctgttg gtcgttttta
     6061 tccggcactg gtcggcaatg atatcggctg cggtatggcg ctatggcaaa cagatattct
     6121 cactcgcaaa tacaacgccg ataagtttga aaagcgatta tcagatctgg atgacgttgc
     6181 tgaagaaagc tggctggagg aaaacctgcc gtcagcattt gcacagcatc cgtggcgcag
     6241 ctcgcttggc tctatcggtg gcggcaatca tttcgctgaa ctgcaacaag ttgatcaaat
     6301 catcaacgct gaactgtttg cactggcagg tctggatgca caacatctgc aactgttggt
     6361 tcatagcggc tcgcgggggg tggggcagtc tattttacag cggcatattg cctcgttctc
     6421 gcatcatggt ttgcctcaag gtagtgacga cgcgctggcg tttgcgtgta ttaatcgcca
     6481 gatgatcgct ttgcgcataa tgcaacaagt taaggccacg ggaagtccgg ttctggatgt
     6541 ggcgcataac tttgttagcg cgtgtcaaat cggcgatcaa cagggctggt tgcatcgtaa
     6601 aggtgccacg ccggatgaca acggtctggt gattattccc ggttcacgtg gtgattactc
     6661 ctggattgta cagcctgttg cgaacgaaaa aacgctccac tcgctggcgc atggggcagg
     6721 gcgtaaatgg gggcgcaccg agtgtaaagg gcgtctggca gcgaaataca cggcgacgca
     6781 actctcgcgt actgaacttg gcagtcgggt aatttgtcgc gataaacaac tcatctttga
     6841 agaagcgcca caagcctata aatcggctga aagcgtggtg caatgtctgg tgcaggccgg
     6901 tttaattatt cctgtcgcgc ggctgcgtcc ggtgctaacg ctcaaaaaca gtggagggaa
     6961 aaaaggatga tcttgctaca actctcctca gcgcaggggc cggaagaatg ttgtctcgca
     7021 gtgaaaaagg cgctggacag gctaattaaa gaagctgccc gacaagacgt cgcggtaacg
     7081 gtgctggaaa cagaaacggg ccactactct gacacgctgc gttcggcgct gatttctctg
     7141 gatggcgata acgcatgggc attaagcgaa agctggtgtg gcactattca gtggatttgt
     7201 ccgagtccgt atcggcctca tcatgggcgc aaaaactggt ttctgggcat tgggcgtttt
     7261 accgctgatg agcaggaaca atcggatgca atccgttatg agacgttgcg ttcgtcgggg
     7321 ccgggcggtc aacatgtcaa taaaaccgac tcggcggtac gcgccacgca tttggcatcc
     7381 ggtattagcg tgaaggttca gtcagagcgt agtcagcatg ctaacaagcg gctggcacga
     7441 ttgctgattg cctggaagct ggagcagcag caacaggaaa atagcgcggt gctgaaatcg
     7501 cagcggcgaa tgttccatca ccagattgaa cgtggcaacc cgagacggac atttacaggg
     7561 atggctttta tcgaaggata atgaaggatg aaactgccgg aaggcgatta aacgccatcc
     7621 ggcagcaaat caaataatta cttcgccgga atttctttca gcagttcagt cagcagtgtc
     7681 cagtaatgac ctacgctttc gatgtgaact tgctcatccg gagagtgtgg accggtgata
     7741 gttggcccga tagaaaccat gtccatttcc ggatacggtt ttttgaacag accacattcc
     7801 aggcccgcgt ggataatctg gatgttcggc gttttgttga acagacgctg ataggtttca
     7861 cgtaccagat gcatcaccgg agaattagcg tccggctgcc agccaggata tgcgcctttc
     7921 gcttcggttt tcgcgccagc cagtttaccc agcgaatcca gcatgctcac cacgtagtct
     7981 ttaccgctgt cgatcagtga acggatcagg cagtgaattt ctacgttatt gtcagtcatg
     8041 gtcaccacac cgacgttcag ggaggtttcg accacacctt ttgccacgtc ggaattgcgg
     8101 atcacaccgt tcggggtggc gttcagcaga cgaataaagg tatcgcgaga tttcgcaatc
     8161 agggcagctt tatcgttcgc tacagagtcc agcaacaagg ccagattttt ctctttctct
     8221 gccagctcgt ttttcaggat ctcctgatag gtattcacca gagatttcag gacgtcgact
     8281 ttatcagctg cgacagcaat ggtcgcaaag gcttcacgcg ggatggcgtt acgcagtgtg
     8341 ccgccgttga aatcgataag gcgcagatcc agttcttccg catgacccgc caggaagcgc
     8401 accagtagtt tgttggcatt acccaggcca acgtggattt caccgccgga gtgaccgcct
     8461 ttcagacctt ttaaggttaa cttgaaggtt tcaaaaccag ctggaaccgc ttcacgatct
     8521 aaatgcaggt tggaggtgaa gtcgataccc cccgcacaac ccatgtagat ttcaccttct
     8581 tcttcggagt cggtgttaat cagaatatca gcctgcaacc agttgctctg taagccgaac
     8641 gcaccgtcca taccggcttc ttcggtcatg gtcagcagca cttccagcgg gccgtgaacc
     8701 acgttttcgt cagccagaac cgccagcgca gacgccatac caatgccgtt atctgcaccc
     8761 agcgtggtgc cgcgcgcttt aacccattcg ccatcaatat aaggctggat aggatctttc
     8821 gtgaagtcat gcacggtgtc gttatttttc tgcggcacca tatcgaggtg ggcctgtaag
     8881 acgaccggtt tacgattttc catacctgcg gtagcaggtt tacgaatcag gatattacct
     8941 acctgatcgc gttcgacatg gaaacctttc tcttttgccc aaccaacaat gtattcagcg
     9001 agttgctctt catgatagga cgggtgagga atagaacaga ttttggcaaa aatatcccac
     9061 agcggctgtg gagataattg agacagttca gacacgttaa gtctccttgt cgatcacccg
     9121 caaaacagta ttgcaggtca cagggttagc agaaaatgtt gtcaacacaa gacaggcttg
     9181 cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga
     9241 cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc
     9301 tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag
     9361 cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg
     9421 actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctggtacc
     9481 gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag
     9541 ctacgatcac gacaaccagc gcgagcttaa agtgctaaaa cgcgcagaag gcgatggcga
     9601 aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga
     9661 aatgtatcca aaagcacatt ttgtgactat cttcgcaaaa ccggctggtc gtccgctggt
     9721 tgatgactat gttgttgata tcccgcaaga tacctggatc gaacagccgt gggatatggg
     9781 cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg
     9841 cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg
     9901 catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg
     9961 aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggctc
    10021 ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc
    10081 gcggcattga cccacgtgaa atcctcgacg tccaggcacg tattgtgatg agcgatgctg
    10141 aacgtaccga tgatgattta tacgataccg tgattggcta ccgtggtggc aactggattt
    10201 atgagtgggc cacccaggcg atggtgtggc aacaaaaagc ctgtgcggaa gaagatccgc
    10261 aactcagtgg tcgacactgg ctgcatgcgg ctacgttgta caacattgcc gcctatcctc
    10321 atctgaaagg agatgacctg gccgagcaag cgcaggcttt atcaaaccgc gcctatgaag
    10381 aggccgccca gcgtctgccg ggcacgatgc ggcagatgga gtttaccgta cccggcggtg
    10441 cgcccatcac cggctttttg catatgccga aaggcgatgg cccgttcccg acagtattaa
    10501 tgtgtggtgg tctggatgcg atgcagacgg actattacag cctgtatgaa cgttattttg
    10561 cgccgcgcgg cattgcgatg ctgactattg atatgccgtc ggtgggcttt tcttcaaaat
    10621 ggaagctcac ccaggactcc agcctgttgc atcagcacgt cttaaaggcg ctgcctaacg
    10681 taccgtgggt ggatcacact cgcgtcgcgg cctttggttt ccgtttcggc gctaacgttg
    10741 ccgtgcgtct ggcatacctt gaatcgccgc gtctgaaagc ggttgcctgt cttggtccgg
    10801 tagttcatac cctgttgagt gattttaagt gccagcaaca ggtgccggaa atgtatcttg
    10861 acgttctggc gagtcgtttg gggatgcatg atgcttccga tgacgcgttg cgcgtggagc
    10921 tgaatcgcta ttcattaaaa gtgcaaggat tgctgggacg tcgctgccca acgccaatgt
    10981 tatcaggcta ctggaagaac gatccgttca gcccggaaga ggactcacgc ttaatcacct
    11041 catcatctgc tgacggtaaa ttattagaga tcccatttaa cccggtgtat cggaattttg
    11101 acaaagctct tcaggaaatc accggctgga tcgaaaaacg cttgtgttaa aaatttgcta
    11161 aattttgcca atttggtaaa acagttgcat cacaacagga gatagcaatg acgttaccga
    11221 gtggacaccc gaagagcaga ttgatcaaaa aatttaccgc actaggcccg tatattcgtg
    11281 aaggtaagtg cgaagataat cgattctttt tcgattgtct ggctgtatgc gtcaacgtga
    11341 aaccggcacc ggaagtgcgt gaattctggg gctggtggat ggagctggaa gcgcaggaat
    11401 cccgttttac ctacagctac cagtttggtc tgttcgataa agcaggcgac tggaagagcg
    11461 ttccggtaaa agacactgaa gtggttgaac gactggagca caccctgcgt gagtttcacg
    11521 agaagctgcg tgaactgctg acgacgctga atctgaagct ggaaccggcg gatgattttc
    11581 gtgacgagcc ggtgaagtta acggcgtgag tgaaatgtgc cggatgcatc acatccggca
    11641 ataatcatta aaactgatag gtcatgccaa ccgcgacaat atcatcatta ttaatattca
    11701 atttgttatc gctatccagt tggttgattt tataatcaac aaacgctgac atatttttgt
    11761 tgaaataata tgtagccccg acatcgatat aattgaccag atcttcatca ccgatacctt
    11821 caatatcttt ccctttcgat aagacataac ccagcgatgg acgcagacca aagtcaaact
    11881 ggtattgagc gaccgcttca aagttctgtg tcttattggc aaagccgcca gttattggcg
    11941 tcattttgcg tgtttcagaa taaaaagttg ccagataaat attattggca tcgtatttca
    12001 gacctgttgc ccatgcttct gcacgcttgc cagtgccacg gctttgcagg ttctgctcgt
    12061 tggtgcgatc tgagttggta taggcaccac taatggcgaa atcgctgccg ccaaagtcat
    12121 atgtcaatga cgtgccgaag ccatcgccgt tttgcttttt aacgtcgcgg ttttcgtttt
    12181 tcccttgata ttgcagggtt aagttcaggc catcgataac gccgaagaag tcggtgttcc
    12241 gatacgtcgc cagaccgctg gcgcgtttgg tcataaagtt gtcggtctgc gccgaggagt
    12301 cgccaccaaa ttccgggaac atatcggtcc aggcttccac gtcatacaac gcgcccaggt
    12361 tacggccata gtcgaaagaa cccaaatctt tatacttcaa tccggcaaaa gcgagacgcg
    12421 ttttttgctg tgcagtatca ctctccgctt tatttccggc aaactccgct tcccaacggc
    12481 catagccagt cagttgatcg ttaatttgtg tttcgccttt aaaaccaaaa cggatataac
    12541 tctggtcgcc atctttactg tcgttatcac tcatataatg catggcttta actttgccat
    12601 agacatccag tttattaccg tctttgttat atatttctgc ggcctgtacg gatgcagatg
    12661 ccacaatgcc catcaccact aatgccagag tgctcttttt cattttcatt cctgatttta
    12721 attaacgcgc gaatattcag cgggagagcc ccgttgaaaa caggaaagtt tttaacctga
    12781 gattgttaaa gatatattac agattaataa tattcttaaa atgtggtaat ttattaaatc
    12841 tgtaataaaa gcgtaaacaa ctgccgctag gcttgctgat cccgcgcaac aaaacgccat
    12901 gctttgcttg cagatggttg gcaaccgacg acagtcctgc taaaacgttc gtttgatatc
    12961 atttttccta aaattgaatg gcagagaatc atgagtgaca gccagacgct ggtggtaaaa
    13021 ctcggcacca gtgtgctaac aggcggatcg cgccgtctga accgtgccca tatcgttgaa
    13081 cttgttcgcc agtgcgcgca gttacatgcc gccgggcatc ggattgttat tgtgacgtcg
    13141 ggcgcgatcg ccgccggacg tgagcacctg gggtacccgg aactgccagc gactatcgcc
    13201 tcgaaacaac tgctggcggc ggtagggcag agtcgactga ttcaactgtg ggaacagctg
    13261 ttttcgattt atggcattca cgtcgggcag atgctgctga cccgtgctga tatggaagac
    13321 cgtgaacgct tcctgaacgc ccgcgacacc ctgcgtgcgt tgctcgataa caatatcgtt
    13381 ccggtaatca atgagaacga tgctgtcgct acggcagaga ttaaggtcgg cgataacgat
    13441 aacctttctg cgctggcggc gattctggcg ggtgccgata aactgttgct gctgaccgat
    13501 caaaaaggtt tgtataccgc tgatccgcgc agcaatccgc aggcagaact gattaaagat
    13561 gtttacggca ttgatgacgc actgcgcgcg attgccggtg acagcgtttc aggcctcgga
    13621 actggcggca tgagtaccaa attgcaggcc gctgacgtgg cttgccgtgc gggtatcgac
    13681 accattattg ccgcgggcag caagccgggc gttattggtg atgtgatgga aggcatttcc
    13741 gtcggtacgc tgttccatgc ccaggcgact ccgcttgaaa accgtaaacg ctggattttc
    13801 ggtgcgccgc cggcgggtga aatcacggta gatgaagggg caactgccgc cattcttgaa
    13861 cgcggcagct ccctgttgcc gaaaggcatt aaaagcgtga ctggcaattt ctcgcgtggt
    13921 gaagtcatcc gcatttgcaa cctcgaaggt cgcgatatcg cccacggcgt cagtcgttac
    13981 aacagcgatg cattacgccg tattgccggg caccactcgc aagaaattga tgcaatactg
    14041 ggatatgaat acggcccggt tgccgttcac cgtgatgaca tgattacccg ttaaggagca
    14101 ggctgatgct ggaacaaatg ggcattgccg cgaagcaagc ctcgtataaa ttagcgcaac
    14161 tctccagccg cgaaaaaaat cgcgtgctgg aaaaaatcgc cgatgaactg gaagcacaaa
    14221 gcgaaatcat cctcaacgct aacgcccagg atgttgctga cgcgcgtgcc aatggccttg
    14281 gcgaagcgat gcttgaccgt ctggcactga cgcccgcacg gctgaaaggc attgccgatg
    14341 atgtgcgcca ggtgtgtaac ctcgccgatc cggtggggca ggtaatcgat ggcagcgtac
    14401 tggacagcgg cctgcgtctt gagcgtcgtc gcgtgccgct gggggtgatt ggcgtgattt
    14461 atgaagcgcg cccgaacgtg acggttgatg tcgcttcgct gtgcctgaaa accggtaatg
    14521 cggtgatcct gcgtggtggc aaagaaacct gtcgcactaa cgcggcaacg gtggcggtga
    14581 ttcaggacgc cctgaaatcc tgtggcttac cggcgggtgc cgtgcaggcg attgataatc
    14641 ctgaccgtgc gctggtcagt gaaatgctgc gtatggataa atacatcgac atgctgatcc
    14701 cgcgcggcgg ggctggtttg cataaactgt gccgcgaaca gtcgacaatc ccggtgatca
    14761 caggtggtat aggcgtatgc catatttacg ttgatgaaag cgcagagatc gctgaagcat
    14821 taaaagtgat cgtcaacgcg aaaactcagc gaccgagcac atgtaatacg gtagaaacgt
    14881 tgctggtgaa taaaaacatc gccgatagct tcctgcccgc attaagcaaa caaatggcgg
    14941 aaagcggcgt gacattacac gcagatgcag ctgcgctggc gcagttgcag acaggccctg
    15001 cgaaggtggt ggcggttaaa gccgaagagt atgacgatga atttctgtca ttagatttga
    15061 acgttaaaat cgtcagcgat cttgacgatg ccatcgccca tattcgtgaa cacggcacac
    15121 aacactccga tgcgatcctg acccgcgata tgcgcaacgc ccagcgtttt gttaacgaag
    15181 tggattcgtc cgctgtttac gttaacgcct ctacgcgttt taccgacggc ggccagtttg
    15241 gtctgggtgc ggaagtggcg gtaagcacac aaaaactcca cgcgcgtggc ccaatggggc
    15301 tggaagcact gaccacttac aagtggatcg gcattggtga ttacaccatt cgtgcgtaaa
    15361 taaaaccggg tgatgcaaaa gtagccattt gattcacaag gccattgacg catcgcccgg
    15421 ttagttttaa ccttgtccac cgtgattcac gttcgtgaac atgtcctttc agggccgata
    15481 tagctcagtt ggtagagcag cgcattcgta atgcgaaggt cgtaggttcg actcctatta
    15541 tcggcaccat taaaatcaaa gagttacccc atatttaaat acaccacgtt tcctcctgtg
    15601 ccgcatttgt gccattgtaa ccttggcaat tcatcaaaat actgttctga catcaggcag
    15661 tgcaggtgca gctatttaaa ccaattgctg ccgccattct ttgacgtagt caatcagggc
    15721 gcggagcttt ggtgcaatat tgcgacgctg tgggaaatac agatagaagc ccggaaattg
    15781 tggaagaaag tcatcaagca gcgatacaag cttaccgctt tcaatatatg gcctgaaagt
    15841 ttcctgagtg gcaattgtta ttcctccgcc ggcaagagcc agcctcaaca tcagacgcag
    15901 atcattagtc gtaatctgcg gttcaatcgc aaggtcgaaa gttctcccgt tttcttcaaa
    15961 tggccagcga taaggcgcaa cctccgggga ctgacgccag ccgatacact tatgggtatt
    16021 tcccccggag gcgagaaagc gctctccacg cccggccgca aggatcagga cgacgggggc
    16081 aggcatgaat cctcctcatg atggagacgt acagaggcga tttctgccag cacggagagt
    16141 gccagagtat gcgcatcccg ggctttgggg aatatcccga cgggggcccg gatttgcgct
    16201 gtctcctccc tggaccatcc cagctcgtgg agcttttgca gacgtaacgt gtgggttcga
    16261 tggctgccca atgcgccgag ataaaagggc tttgcttctc gcgcggcctg caatgctggc
    16321 agctcccggt tgagatcatg gcacagcaaa atgaccgccg tgtcggtatc gatctgagcg
    16381 ctggctgagg ccggaaaaag atcgaagata tggctgtcat agcctgtggc tgctgcaaga
    16441 ctcgcggttg cctgcgcctc aagagaacgt ccgtaaatca tcagcctgac gcatggcctg
    16501 aaccccacct caaagccatt gagattccag cccgttcggg tttgcgtggg caggcacacc
    16561 aacgattgtg cttgcggatc gtagcgcagc cccgccggtt ttctctgttc cagacggttc
    16621 agcacggcga gcagaggctg tgccgagcgt agtttatgga gcgtaagcgt gatcccaccg
    16681 ccgcagggca gaacgatgtc aaaccacggc gaaccttcgc catagcgaat ttcgcgatcg
    16741 cggcctgagc ccatcatctc cagcgcttca aaagcggcag cggcctccac gcagccgcca
    16801 gagacaaaac cgcagtaacg accatcttcg cgaaccacca tctgcgcccc gagcgggcgc
    16861 gccgcgccgc cgtgtatctc caccagcgtc accagcaccg ctcctttacc ggacattagc
    16921 gcctctacgg cgaagcgcag aatggtccgg tggtcatcgg tgagaaacgc ctgctctggc
    16981 ttatgccagt gttcgtcttt atcaaaaagc gggtatgaca ttgtttctcc ttaaaccaca
    17041 tccggcagct tatcgagcag cttatccaga gtgatgggat aatcccgtac ccgaataccg
    17101 gtggcgttat acaccgcgtt ggcgatagcc gcgctcacgc cgcacaggcc caactcaccg
    17161 acacctttgg ccttcatcgg ggaggatatg gggtcggtat catccaggaa aatcacctcc
    17221 tgttttggga tatccgcatg gaccggcacc tcataccccg ccatatcgtg attaacgaag
    17281 tagcccaaac ggtcatccac cgccagctcc tccatcagtg ccgcgcccat gcccatagtc
    17341 attgcgccaa tgacctggct gcgcgcagtt ttcggattca ggatgcgtcc tgcagcacac
    17401 acagcgagca tacgccggac ccgaacttct cccgtcgcgc tatgcacgcc gacctccaca
    17461 aaatgcccgg caaaggtcga ctgctggtac tctttgctca gtgttccgaa ttcaatgctc
    17521 tcttccgctg tcagtctgcc gcctgcggtg gcttcatgta gcgtggcgct tcgggtaccg
    17581 ttggtaatct tgccgtcggc aaactgcgac tgctcaggat caaacccgac tgccgaggca
    17641 atcatttcgc gaagcttcat acaggcggcg taaacgccgg aggtggaggt attcgcgccc
    17701 cattgtccac cagaacccgc agaaaccggg aaactggaat cgccgaggtg aaccgcaacc
    17761 tgctccagcg gtacgccaag catttccgct gccgtctggg ccagaatggt gtagctgccg
    17821 gtgccaatgt cggtcatgtc cgtttctacg gtaacggtgc cgttttgttc gaggtgaacc
    17881 cgagcacccg atttttccag cagattattg cgaaagcccg ccgcaacacc gtggccgact
    17941 agccactccc cgtcgcgcac ctgtccgggt gtggcgttgc gctgcttcca gccaaattta
    18001 tccgctccgg tgcgcaagca ctcgataagc tgacggcgag agaagcagcg cgtcgggtcg
    18061 gcggggtcaa cctgagtgtc attcaggatg cgaaactcga cgggatcgat gcccgctttt
    18121 tccgccagtt cgtcgatcgc gatttcgagc gccatcagac cgggggcttc gccgggcgca
    18181 cgcatggcgt tcccttccgg caaatcaagc gtggcgagcc gcaggccggt atgacgattc
    18241 gccccggcgt agagtaattc gctttgctgt accgccgttt ccggcgtgcc gccgggcagg
    18301 tttccagacc agctttcatg tgagatagcg gtgattttcc cgctctggtc ggcaccgata
    18361 cgcaagtgct gaagggtggc g
//
LOCUS       DAZGVQ010000099        18340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000099 DAZGVQ010000000
VERSION     DAZGVQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.100"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..580)
                     /gene="rsmG"
                     /locus_tag="ACHLAG_003318"
     CDS             complement(<1..580)
                     /gene="rsmG"
                     /locus_tag="ACHLAG_003318"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFP0656485.1"
                     /translation="MLNKLSSLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPAL"
     gene            complement(644..2533)
                     /gene="mnmG"
                     /locus_tag="ACHLAG_003319"
     CDS             complement(644..2533)
                     /gene="mnmG"
                     /locus_tag="ACHLAG_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFP0656486.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGGIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2912..3355)
                     /gene="mioC"
                     /locus_tag="ACHLAG_003320"
     CDS             complement(2912..3355)
                     /gene="mioC"
                     /locus_tag="ACHLAG_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFP0656487.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3445..3903)
                     /gene="asnC"
                     /locus_tag="ACHLAG_003321"
     CDS             complement(3445..3903)
                     /gene="asnC"
                     /locus_tag="ACHLAG_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFP0656488.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4055..5047
                     /gene="asnA"
                     /locus_tag="ACHLAG_003322"
     CDS             4055..5047
                     /gene="asnA"
                     /locus_tag="ACHLAG_003322"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFP0656489.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(5052..6503)
                     /gene="viaA"
                     /locus_tag="ACHLAG_003323"
     CDS             complement(5052..6503)
                     /gene="viaA"
                     /locus_tag="ACHLAG_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFP0656490.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMEAFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6497..7993)
                     /gene="ravA"
                     /locus_tag="ACHLAG_003324"
     CDS             complement(6497..7993)
                     /gene="ravA"
                     /locus_tag="ACHLAG_003324"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFP0656491.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8216..10084
                     /gene="kup"
                     /locus_tag="ACHLAG_003325"
     CDS             8216..10084
                     /gene="kup"
                     /locus_tag="ACHLAG_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFP0656492.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10251..10670
                     /gene="rbsD"
                     /locus_tag="ACHLAG_003326"
     CDS             10251..10670
                     /gene="rbsD"
                     /locus_tag="ACHLAG_003326"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFP0656493.1"
                     /translation="MKKGTVLNSDISSMISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10678..12183
                     /gene="rbsA"
                     /locus_tag="ACHLAG_003327"
     CDS             10678..12183
                     /gene="rbsA"
                     /locus_tag="ACHLAG_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFP0656494.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLASGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12188..13153
                     /gene="rbsC"
                     /locus_tag="ACHLAG_003328"
     CDS             12188..13153
                     /gene="rbsC"
                     /locus_tag="ACHLAG_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFP0656495.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGININK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13178..14068
                     /gene="rbsB"
                     /locus_tag="ACHLAG_003329"
     CDS             13178..14068
                     /gene="rbsB"
                     /locus_tag="ACHLAG_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFP0656496.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14194..15123
                     /gene="rbsK"
                     /locus_tag="ACHLAG_003330"
     CDS             14194..15123
                     /gene="rbsK"
                     /locus_tag="ACHLAG_003330"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP0656497.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15127..16119
                     /gene="rbsR"
                     /locus_tag="ACHLAG_003331"
     CDS             15127..16119
                     /gene="rbsR"
                     /locus_tag="ACHLAG_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFP0656498.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16085..17512)
                     /gene="mdtD"
                     /locus_tag="ACHLAG_003332"
     CDS             complement(16085..17512)
                     /gene="mdtD"
                     /locus_tag="ACHLAG_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFP0656499.1"
                     /translation="MSDKKNRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(17535..18227)
                     /gene="yieP"
                     /locus_tag="ACHLAG_003333"
     CDS             complement(17535..18227)
                     /gene="yieP"
                     /locus_tag="ACHLAG_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFP0656500.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4406 a         4548 c         4796 g         4590 t
ORIGIN      
        1 ccagggctgg aacctgaagt ttaaccactg attcgacctg atattcttcg ggcaacaaag
       61 cgatttcatc ttccggcatt tgccctttca gcgcgtagaa acggccttgc tcaccaggaa
      121 gatggtggca ccagctcacc atatcgttca gagaggcaaa agcgcggcta attacgccat
      181 caaatggcgg ctctgaagga aactcttcta ccctgctctg tactggttca atattctcca
      241 gtttaagctc atgttgcacc tgacgaagga aacgcacgcg tttaccaagg ctatccaaca
      301 gagtgaaatg ggcttcagga cgcacgatag agagtggaat gcctggcagt cctggtccgg
      361 tgccgacatc gataaaccgt tcaccttgca gatacggtgc caccacaatg ctatcgagaa
      421 tatggcgtac cagcatctca ttaggatcgc ggaccgaagt caggttgtac gctttgttcc
      481 atttatgcag catattcacg taggcaataa gctggttttt ctggtgatcg gtaagcgaaa
      541 tacctgcgtc tttcagcagt gaggagagtt tgttgagcac ggtgattacc tgttcttgat
      601 gcgttgcctg gtaagcgggt gcttaccagg catttttaat gcgttatgcg ctacgacgca
      661 gcataccctg ttttttcagc cacaccagca gaatggagat ggccgcaggc gtgacgccag
      721 aaatacgcga agcttggccg atagaggctg gtttgtgatc gttaagtttg gcgatcactt
      781 cgttagaaag accggatacc tggcggtaat ccagtgtcgc gggtagcagg gtgttctcgt
      841 tacgcagctg cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg tatttaacct
      901 gaatctcaac ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac ggcgtcagcg
      961 tggttaattt ttcataagtc atttccggac gacgcagcag atcttcacca ctggcttcac
     1021 gggaaagcgg cgcagtcagg tgagcattca cttcggctgc agcttccgcc gacggggtta
     1081 cccaggtcga tttcagacgc tgacgctcac gctcgatatt ctcaagtttc tcgttaaagc
     1141 gcgcccaacg ttcgtcatcc accaggccca gttcacgacc gatttcagtc aaacgcagat
     1201 ccgcattatc ttcgcgtagc atcagacgat attctgcgcg cgaagtaaac atacgatacg
     1261 gttctttggt tcctaaagtg cacaggtcat caactagtac gccgagatac gcctgagaac
     1321 gtgccggagc ccaaccttct ttgtcagcag acagacgggc agcgttaaga ccggccagca
     1381 aaccttgcgc agcggcttct tcgtaaccgg tagtgccgtt aatctgacca gcaaagaaca
     1441 gcccctggat aaacttgctc tccagcgtcg gtttcaggtc gcgaggatcg aagaagtcat
     1501 actcaatggc ataacccgga cgcacgatct tcgcgttttc catcccctgc atagagcgga
     1561 cgatttgcat ctgcacatcg aacggcaggc tggtggagat accgttcgga taaatttcat
     1621 tagaggtcag tccttccggt tcaaggaaga tctgatgctg atttctgtcg gcgaagcgca
     1681 taactttgtc ttcgatcgac gggcagtagc gtgggccgac accttcgatc ccccctgcat
     1741 acattgggct acgatcgagg ttactgcgga tcacatcatg ggttttctcg ttggtatgag
     1801 tgatataaca cggcacctgc tggggatgct gggacgcatt gcccataaac gagaataccg
     1861 gcattgggtt atcgccatgc tgttgcgcca gcacactaaa gtcgatagtt cgagcatcaa
     1921 tacgcggtgg tgtcccggtt ttcagacgac caacgcgcag cggcagttca cgcaaacggc
     1981 gagaaagcgg aatggacggc ggatcaccag cacggccacc gctgtaatta tccagaccga
     2041 tatgaatttt accgtcgagg aacgtcccaa cggtgagcac gacggctttg gcacggaact
     2101 tcagtcccat ttgggtaaca gcaccgacca cgcgatcgtt ttcgacaata agatcttcaa
     2161 ccgcctgctg gaagatcatc aggttcggtt ggttctccag cgccgtacgt accgcctgac
     2221 ggtagagcac acgatccgcc tgagctcggg tagcgcgaac cgccggtcct ttgcttgcgt
     2281 ttagtatcct aaactggata cccgcttgat cgatcgcttt cgccatcaga ccgccgagtg
     2341 catccacttc ttttaccaga tgtcccttcc caataccgcc gatcgccggg ttgcagctca
     2401 tctgccccag agtgtcgata ttgtgtgtca aaagcagagt ctgttgaccc atacgcgccg
     2461 cggccatcgc ggcctcggtg cctgcatgac ccccgccaat gatgatgacg tcaaaaggat
     2521 ccggataaaa catggtgatt gcctcgcata acgcggtatg aaaatggatt gaagcccggg
     2581 ccgtggattc tactcaactt tgtcggcttg agaaagacct gggatcctgg gtattaaaaa
     2641 gaagatctat ttatttagag atctgttcta ttgtgatctc ttattaggat cgcactgccc
     2701 tgtggataac aaggatccgg cttttaagat caacaacctg gaaaggatca ttaactgtga
     2761 atgatcggtg atcctggacc gtataagctg ggatcagaat gaggggttat acacaactca
     2821 aaaactgaac aaccgttgtt ccttggataa ctaccggttg atccaagctt cctgacagag
     2881 ttatccacag tagatcgcac gatctgtata cttatttgag taaattaatc cacgatccca
     2941 gccattcttc tgccggatct tccggaatgt cgtgatcaag aatgttgatc ttcagtgttt
     3001 cgcctgtctg ttttgcaccg gaatttttga gttctgcctc gagtttatcg atagccccac
     3061 aaaaggtgtc atattcacga ctgccaatac cgattgcgcc aaagcggacc gcagaaagat
     3121 cgggcttctg ttcctgcaat gcttcataga aaggagaaag gttgtccgga atatctccgg
     3181 caccgtgggt ggagctgata atcagccaga tccccgaggc agataaatct tccaacagcg
     3241 gaccgtgcag cgtttcggtg gtaaaacccg cctcttccag cttttcagcc aggtgttctg
     3301 ctacatattc ggcaccgccg agggtgctgc cgctgataag agtgatatct gccataaacc
     3361 gccaccttta ttaagagtgg cgtattgtac gctgtgaacg cgttgggatc tacctgtgga
     3421 aaagtatggg attaaaaaag ccgattatgg cttgatggta cgcatgatcg ggttttgcag
     3481 gacgatcaat gtctcggtgg actgaatttc atcaattgtt tggatcttgt tgataagtac
     3541 atgctggaga gcgtcgatcg aacggcacat cacttttata aagatgctgt agtggccggt
     3601 tgtgtaatag gcttcagtga cttcatcaag gctttccagc tttgccagcg cggaagggta
     3661 gtctttggcg ctctttaata taatgccgat aaagcagcct acgtcataac cgagctgctt
     3721 cgggctgaca tcaatacgcg ccccggtaat gatccccgcc tgcttcattt tctctactcg
     3781 aacgtgaatc gtccccggac tgacgccaaa ttgtttcgcc agttcggcgt aagcggtgcg
     3841 cgcattgccc attaatgctt ccaggatgcc acggtccaga ttgtcgatca gataattttc
     3901 cataggattt tcttatgcag attgatgatt cattctattt tagccttctt ttttaatgaa
     3961 tcaaaagtgt gttaggcttt ttattgaatg attattgcat gtgtgtcggt ttttgttgct
     4021 taatcataag caacaggacg caggagtata aaaaatgaaa accgcttaca ttgccaaaca
     4081 acgccaaatt agcttcgtga aatctcactt ttctcgccaa ctggaagaac gtctggggct
     4141 gatcgaagtc caggcgccga ttcttagccg tgtgggggat ggcacgcagg ataacttgtc
     4201 gggctgtgaa aaagcggtgc aggtaaaagt gaaagctctg cctgatgccc agttcgaagt
     4261 ggttcattca ctggcgaagt ggaaacgtca gaccttaggg caacacgact tcagcgcggg
     4321 cgaagggctg tacacgcaca tgaaagccct tcgccccgat gaagagcgtc tttctccgtt
     4381 gcactcggtc tatgttgacc agtgggactg ggaacgcgta atgggcgacg gtgagcgtca
     4441 attctcgact ctgaaaagca cggtagaggc gatctgggcg ggaattaaag caaccgaagc
     4501 tgcagttagc gaagagtttg gtttggcacc gttcctgccg gatcagatcc acttcgtaca
     4561 cagccaggag ttactgtctc gttatccgga tctcgatgcc aaagggcgtg agcgggcgat
     4621 tgcgaaagat cttggcgcgg tattcctcgt cgggattggc ggcaagctga gtgatggtca
     4681 tcgtcacgac gtgcgcgcac cggattatga tgactggagc accccgtcag agctgggcca
     4741 tgcgggtctg aacggcgata ttctggtatg gaaccctgta ctggaagatg cgtttgagct
     4801 ttcttccatg gggatccgcg tggatgccga cacgttgaag catcagttgg cgctgaccgg
     4861 tgatgaagat cgtctgcagc tggaatggca tcaggcgctg ctgcgcggtg aaatgccgca
     4921 gaccatcggc ggcggtatcg gccagtctcg tttgaccatg ctgctgctgc aactgccgca
     4981 tatcggccag gttcagtgtg gtgtatggcc agctgctgtt cgcgagaacg tcccttctct
     5041 gctgtaataa tttatcgccg ccagcgtctg agcaggcggc ttcgcatccc ggtatcaaag
     5101 cgccagatat gatcgaaaat gcgcatgatg ccgggtttgc cgtgtgccga catcgccacg
     5161 gcatgaaagc gatgctgatg tacccgctgc agctctttca ctttactcgt cacgtcgtca
     5221 ggcaaccgct gagcgataaa atcagaaatc accaccgcgt cggcatcaaa ccattccctg
     5281 ctttgcaagc gttccataat ggcgcgaaaa caactggcaa gatcggtgcc gccacgaaac
     5341 tgctggctta aaaaacggat tgcttgttcg atgccttgtg ggcctgaaag ctcataacgg
     5401 acgatctcgg tggaaaatag cataatatag cagcgccggt tttcagcgag agcaatgcgc
     5461 atcaaggcca ggcagaacgc tttcgcacac tgttcattaa agccgcccat tgagccggaa
     5521 gtatccacac agacaataaa cggcccgcgc ggctgttcat cgtaatcttt atgtaccacc
     5581 ggacgttcga tcactttttc acgccacgac tcaccgtgca ggcgataggt gagcaactgt
     5641 ttttccacca gccgacggta aaactcatac tccagttccg ttatccctag tgtcgccagt
     5701 tctggcggca ggagacgtaa aatatcatcg ctttgttgca gaccatcaac ctgctcagga
     5761 acagttgccg gttcgcgcac catggtgcgg aaggcttcca tctgcgcatc gttacgcggt
     5821 attgatttgg cttcccggga acgccccagc tgttctgcca ggcgtttcag ttccggctgt
     5881 tcgttaagaa attcaccgta tttcacaatc aactgatagt cgccacgttt aagctgaccg
     5941 gcgctcatat cccacagacg accagctgcg gtattgttat ctgcgagaat cggttcaagt
     6001 tgtccgctca gcgtcatgcg ttcctgaact tcacttaaca gttgttcgcg ttcttcttct
     6061 aatagctgtt gatttaacgt cgttgcttgc acgatcagac ttaaacgcca acgctggagg
     6121 aaaagcgtat gtaacgctga agtgatcgtg ctgttagcat caaccaactg tcgggcttgt
     6181 tctgcccatg gggaattcag acgatgcagt aagtccagga tctgtggtag ctgcacaata
     6241 aactgcggcg tggagaggag ctggctttgc tgatagcaca tcacctcttc ggtgagttcc
     6301 ggcgggactc gggcatcttt cagccgactg cgcagcgcct cacgccagcg gggaacatca
     6361 tcagtgattg ccgccttcag tcgtgggaat ttttcaaaga agactgccag ctgcggtgag
     6421 gccagcagcg cgatgatcat ctcttcgatc aatccctctt cgctgacggc cagcatcaca
     6481 ttaagcgtat ccagcgttag cattgttgtg cctggcgaat ctgtgcgccg acatcctgta
     6541 ggctggcttc aatgcgacct aaccagtcgc cagggataaa cagacatttt tgctgttcgc
     6601 tgaataaagc gtgttgcttg cgccagtcac tttcaagctc ttccagttgt tgttttattt
     6661 cacctggcag accttcagcc gatgaaccgg gaagtgccag cgtactgcct tgtaaactta
     6721 catcgcgtac aacaagatgt tgggcgctat ccacttccag attcaatttc tgggcaaagc
     6781 cgataccgtt cagtttgccg cgaatttcac cacctttact cagccactgt tccagcgcgt
     6841 tacgctcaaa ggagatatga accacttcca tatcatgcaa ttttaacggt ttttgcagca
     6901 gcagagtcag agtggaagca gtaacgttaa caggaagttg atactgctga cgacggctga
     6961 aaatgccgcc cagacgaatt accgttaagg ctgttttatc gctttgttgc tgctggagtt
     7021 gcaggtgacg ttgcacaatc gcgccaaggc gagtcaacat cccttgctgt tgccaggcgt
     7081 gaccggtcat caatacatca atttgttgtt gtatcaaatt caggctttgc gcgtcatacc
     7141 acaggcaatc tttcagcaaa atgagatcta ccggggcaac agcactgcga ccgctaaaaa
     7201 aggcgctggc ctgcaataat cggatcgctt ttttccagcg acgatccgag acataaggcg
     7261 catccggtaa tttatccagt tgctggcgca gcataaaaat cagctcaaat acatgatcgg
     7321 gcagcgtaat ttcaccaatc tctttctgcc agcgttcata ttcttcatct gtgacctgca
     7381 aggcatcagg aaccggattg tcgttttcat cctgttgact ggtcagcatg gagcggaaat
     7441 tcgctttatc ctgcacttta tctaaccaca gacgaatcag catgcggtca tataacgctt
     7501 ccagactgct gtctgcttcc ggcagctcgt tggaggccgc caccagcaga cgcatcggga
     7561 ttttttctac gtgtgcaccg ttgcggaact ggcgctcgtt aatggcggtg agcaaggtat
     7621 taagaattgc cgggcccgct ttccagatct catccagaaa gacaatttct gcttccggca
     7681 ggtaaccgct ggttaaacgt tcatagcgcc cttcatcttt tagcgcctga atagaaaggg
     7741 gaccaaaaac ttcttccggc gtggagaagc gggtcatcag atattcaaac gcgcgggcat
     7801 tctgaaaggc gaattttaag cgccgggcga tcaaactttt ggcaatacct ggcgggccaa
     7861 ggaggaacac actttcacca cttaatgccg ctaataaaca caagcggatg gcgtggctac
     7921 gttcataaag ccccttttcc aacgaactgc tcaggcggga aattctttcc gctaataaat
     7981 gagggtgagc cataatgaag tggcgtcctt tcgtcaaaag ttctgcgtaa attgcgagta
     8041 tagacgtttc ttgctggtgg ctaaaatagt ctcaaagggg gggtattttt ctttgagcca
     8101 ggttaatgtg gccgcattta ggagtacgat tttgccgtta atcgtgcata ctgtgcgctt
     8161 ttttgtgggc caagggacta agcacacatt tcatatttca acgaaagact agtctatgag
     8221 cactgataat aagcaatcat tgcccgcgat taccctcgcg gcgattggag ttgtctacgg
     8281 cgatattggt accagcccgt tatatacact tcgtgaatgt ttgtccggcc agtttggttt
     8341 tggcgttgaa cgcgatgccg tgtttggctt tttatcgctg atcttctggc tgctaatctt
     8401 tgtggtttcc attaaatatc tcaccttcgt gatgcgggca gataacgccg gtgagggggg
     8461 gatcctgacg ttgatgtcgc ttgccgggcg taatacgtcg gcgcgaacca catcaatgct
     8521 ggtgattatg gggctaatcg gcggcagctt tttctatggt gaagtcgtca taacacccgc
     8581 tatttcggtg atgtcagcca ttgaaggtct ggaaatcgtc gccccgcagc tggatacctg
     8641 gatagttccc ctctcaatta tcgttctcac tttattattt atgattcaaa aacatggcac
     8701 ggctatggtc ggtaagctgt ttgcgccgat catgctgacc tggtttttga ttctggcagg
     8761 actggggtta cgtagcatta ttgctaaccc ggaagtgctg catgcgctga atccaatgtg
     8821 ggcggtgcat ttcttccttg aatacaaaac ggtttctttt attgcattag gggcagtggt
     8881 gttgtcgatt acgggggtcg aggcgctgta tgctgatatg gggcactttg gtaagttccc
     8941 tattcgcctg gcgtggttca ccgtcgtatt accttccttg acccttaatt acttcggtca
     9001 gggagcgctg ttgttaaaga acccggaagc gattaagaac ccgttcttcc tgttggcacc
     9061 ggactgggcg ctgatcccgc tgctgatcat cgccgcactg gcgacggtaa tcgcctcgca
     9121 ggctgtcatc tctggcgtct tctcattgac gcgtcaggcg gtacgtctgg gatatttgtc
     9181 gccgatgcgc attattcaca cctccgaaat ggagtcaggg caaatctata ttccgtttgt
     9241 gaactggatg ctctatgtcg cggtcgtgat tgtgattgtc agctttgagc actccagcaa
     9301 cctggcggcg gcgtacggga ttgcggtgac cggaaccatg gtgctgacgt ctattctctc
     9361 gactactgtg gcacgtcaga actggcactg gaataagtat tttgttgcgc tgatcctgat
     9421 tgctttcctt tgtgtcgata ttccattgtt caccgctaac ctcgataaac tgctctccgg
     9481 cggctggttg ccattgagcc tcggtactgt gatgtttatc gtgatgacca cctggaagag
     9541 cgagcgtttc cgcttgctgc ggcggatgca tgaacatggt aactctctgg aagcgatgat
     9601 tgcttcgctg gagaaatcac cgcctgttcg cgtgcccggg accgcggtgt atatgtcgcg
     9661 tgcaatcaac gtcattccct ttgcgctgat gcataacctt aaacataaca aggtattgca
     9721 tgagcgggtg attctgttaa ctctgcgcac cgaagacgcg ccatatgtcc ataacgtccg
     9781 tcgggtacag atagaacaac tgtcgcccac tttctggcgc gtggtggcaa gttatggttg
     9841 gcgagaaacg ccaaacgtgg aagaagtttt ccaccgctgc ggtctggaag gattaagttg
     9901 ccggatgatg gaaacctcct tctttatgtc gcatgagtcg ttgatcctcg gcaaacgccc
     9961 gtggtatttg cgtctgcgcg gcaagctgta cttgctgctg caacgtaatg cgctgcgtgc
    10021 accagatcaa tttgaaattc cgccaaacag ggttatcgaa ctgggtactc aggtcgaaat
    10081 ctaacgccag acgcctcctt tctccataag ggggcgtttt tgttttcatg gttaatcacc
    10141 atgtaaaacg tttcgaggtt gatcacattt ccgtaacgtc acgatggttt tcccaactca
    10201 gtcaggatta aactgcgggt cagcgaaacg tttcgctgat ggagaaaaaa atgaaaaaag
    10261 gcaccgttct taattctgat atttcatcga tgatctcccg tctgggacat accgatacgc
    10321 tggtggtgtg tgatgctggt ttacccatcc ccaaaagtac aacgcgtatc gatatggcat
    10381 taacccaggg tgtaccttct tttatgcagg tgctgggcgt cgtcacaaat gaaatgcagg
    10441 tcgaggctgc cattatcgcg gaagagatca aacaacataa tccgcaactc cacgaaacgt
    10501 tgctcactca ccttgagcag ctgcaaaaac accagggaaa taccattgaa attcgttaca
    10561 ccacgcatga acaattcaaa caacaaaccg cagaaagtca ggcggtaatt cgcagcggag
    10621 aatgttctcc gtatgcgaat atcattctct gtgctggcgt gacgttctga ggccgtcatg
    10681 gaagcattac ttcagcttaa aggcatcgat aaagccttcc cgggcgtaaa agccctctcg
    10741 ggcgcagcgt taaatgtcta tccgggccgc gtgatggcgc tggtgggcga aaacggcgcg
    10801 ggtaaatcca ccatgatgaa agtgcttact ggtatctatg ctcgcgatgc cggcacgctt
    10861 ttatggctgg ggaaagaaac gacatttacc gggccgaaat cttcccagga agccgggatt
    10921 gggattatcc atcaggaact gaacctgatc ccgcagttga ccattgccga aaacattttc
    10981 ctcggtcgtg agtttgttaa tcgctttggc aaaattgact ggaaaaccat gtatgccgaa
    11041 gcggataaat tgctggctaa acttaacctg cgctttaaaa gcgacaagct ggtgggcgat
    11101 ctttccatcg gtgaccagca aatggttgaa atcgccaaag tgctgagctt tgagtcgaaa
    11161 gtcatcatta tggatgaacc gaccgatgcg ctgaccgata ccgaaaccga atccctgttc
    11221 cgcgtcatac gcgagctgaa atcgcaaggc cgcggtattg tctatatctc ccaccgcatg
    11281 aaagaaatct tcgagatttg cgatgacgtt accgtttttc gtgacgggca atttattgct
    11341 gagcgcgaag tggcatcact gaccgaagat tcgctgattg agatgatggt gggccgcaag
    11401 ctagaagatc aatatccaca cctggacaaa gcgccgggag atatccgcct gaaagtcgat
    11461 aatctctgcg gacctggcgt taacgatgtc tcttttactt tacgcaaagg cgaaattctt
    11521 ggcgtctctg gtttgatggg cgcaggtcgt accgaactga tgaaagtgct ctacggcgca
    11581 ctaccgcgca ccagcggtta cgtcaccctg gatgggcatg aagtcgttac ccgttcaccg
    11641 caggatggcc tggcaagcgg cattgtgtat atctccgaag accgtaaacg tgacggttta
    11701 gtgttgggca tgtcagtaaa agagaacatg tcgctgacag cgctgcgcta cttcagccgc
    11761 gctggcggca gtttgaagca tgccgatgaa cagcaggctg tgagtgattt cattcgtctg
    11821 tttaatgtga aaacaccgtc gatggaacag gcaattggtc tgctttccgg tggcaatcag
    11881 caaaaagtgg cgattgcccg cggtctgatg acacgcccca aagtgttgat ccttgatgaa
    11941 cctacccgtg gcgtagatgt cggcgcgaaa aaagagatct atcaactgat taaccagttc
    12001 aaagccgatg gcttgagcat cattctggtg tcatcggaga tgccagaagt attaggcatg
    12061 agcgatcgca tcatcgtcat gcatgaaggg catctcagcg gggaatttac tcgtgagcag
    12121 gccacccagg aagtgttaat ggctgccgct gtgggcaagc ttaatcgcgt gaatcaggag
    12181 taaaaaaatg acaacccaga ctgtctctgg tcgccgttat ttcacgaaag cgtggctgat
    12241 ggagcagaaa tcgcttatcg ctctgctggt gctgatcgcg attgtctcga cgttaagccc
    12301 gaactttttc accatcaata acttattcaa tattctccag caaacctcgg tgaacgccat
    12361 tatggcggtc gggatgacgc tggtgatcct gacgtcgggc atcgacttgt ctgtaggttc
    12421 tctgttggcg ctaaccggcg cagttgctgc atctatcgtc ggcattgaag tcaatgcgct
    12481 ggtggctgtc gctgctgctc tcgcgttagg tgccgcaatt ggtgcggtaa ccggggtgat
    12541 tgtagcgaaa ggtcgcgttc aggcgtttat cgctacgctg gttatgatgc ttttactgcg
    12601 cggcgtgacc atggtttata ccaacggtag cccagtgaat accggcttta ctgagaacgc
    12661 cgatctgttt ggctggtttg gtattggtcg tccgctgggc gtaccgacgc cagtctggat
    12721 catggggatt gtcttcctcg cggcctggta catgctgcat cacacgcgtc tggggcgtta
    12781 catctacgcg ctgggcggca acgaagcggc aacgcgtctt tctggtatca acatcaataa
    12841 aatcaaaatc atcgtctatt ctctttgtgg tctgctggca tcgctggccg ggatcattga
    12901 agtggcgcgt ctctcctccg cacaacccac ggcggggact ggctatgagc tggatgctat
    12961 tgctgcggtg gttctgggcg gtacgagtct ggcgggcgga aaaggtcgca ttgttgggac
    13021 gttgatcggc gcattaattc ttggcttcct taataatgga ttgaatttgt taggtgtttc
    13081 ctcctattac cagatgatcg tcaaagcggt ggtgattttg ctggcggtgc tggtagacaa
    13141 caaaaagcag taataacgac tacaggacat cttgaatatg aacatgaaaa aactggctac
    13201 cctggtttcc gctgttgcgc taagcgccac cgtcagtgcg aatgcgatgg caaaagacac
    13261 catcgcgctg gtggtctcca cgcttaacaa cccgttcttt gtatcgctga aagatggcgc
    13321 gcagaaagag gcggataagc ttggctataa cctggtggtg ctggactccc agaacaaccc
    13381 ggcgaaagag ctggcgaacg tgcaggactt aaccgttcgc ggcaccaaaa ttctgctgat
    13441 taacccgacc gactccgacg cagtaggtaa tgctgtgaag atggctaacc aggcgaacat
    13501 cccggttatc actcttgacc gccaggcaac gaaaggtgaa gtggtgagcc acattgcttc
    13561 tgataacgta ctgggtggca aaatcgccgg tgattacatc gcgaagaaag cgggtgaagg
    13621 tgccaaagtt atcgagctgc aaggcattgc tggtacatcc gcagcccgtg aacgtggcga
    13681 aggcttccag caggccgttg ctgctcacaa gtttaatgtt cttgccagcc agccagcaga
    13741 ttttgatcgc actaaaggtt tgaacgtaat gcagaacctg ttgaccgctc atccggatgt
    13801 tcaggctgta ttcgcgcaga atgatgaaat ggcgctgggc gcgctgcgcg cactgcaaac
    13861 tgccggtaaa tcggatgtga tggtcgtcgg atttgacggt acaccggatg gcgaaaaagc
    13921 ggtgaatgat ggcaaactag cagcgactat cgctcagcta cccgatcaga ttggcgcgaa
    13981 aggcgtcgaa accgcagata aagtgctgaa aggcgagaaa gttcaggcta agtatccggt
    14041 tgatctgaaa ctggttgtta agcagtagtt ttaatcaggt tgtatgacct gatggtgaca
    14101 taaatacgtc atcgacagat gaacgtgtaa tataaagaaa agcagggcac gcgccaccct
    14161 aacacggtgg cgcattttat ggacatcccg aatatgcaaa acgcaggcag cctcgttgtt
    14221 cttggcagca ttaatgctga ccacattctt aatcttcaat cttttcctac tccaggcgaa
    14281 accgtaaccg gtaaccacta tcaggttgca tttggcggca aaggcgcgaa tcaggctgtg
    14341 gctgctgggc gtagcggtgc gaatatcgcg tttattgcct gtacgggtga tgacagcatt
    14401 ggtgagagcg ttcgccagca gctcgccact gataacattg atattactcc ggtcagcgtg
    14461 atcaaaggcg aatcaacagg tgtggcgctg atttttgtta atggcgaagg tgagaatgtc
    14521 atcggtattc atgccggcgc taatgctgcc ctttccccgg cgctggtgga agcgcaacgt
    14581 gagcgtattg ccaacgcgtc agcattatta atgcagctgg aatcaccact cgaaagtgtg
    14641 atggcagcgg cgaaaatcgc ccatcaaaat aagactatcg ttgcgcttaa cccggctccg
    14701 gctcgcgaac ttcctgacga actgctggcg ctggtggaca ttattacgcc aaacgaaacg
    14761 gaagcagaaa agctcaccgg tattcgtgtt gaaaatgatg aagatgcagc gaaggcggcg
    14821 caggtactgc atgaaaaagg tatccgtact gtactgatta ctttaggaag tcgtggtgta
    14881 tgggctagcg tgaatggtga aggtcagcgc gttcctggat tccgggtgca ggctgtcgat
    14941 accattgctg ccggagatac ctttaacggc gcgttaatca cggcgttgct ggaagaaaaa
    15001 ccattgccag aggcgattcg gtttgcccat gctgccgctg cgattgccgt aacacgtaaa
    15061 ggcgcacaac cttccgtacc gtggcgtgaa gagatcgacg catttttaga caggcagagg
    15121 tgacgcttgg ctacaatgaa agatgttgcc cgcctggcgg gcgtttctac ctcaacagtt
    15181 tctcacgtta tcaataaaga tcgcttcgtc agtgaagcga ttaccgccaa agttgaagcg
    15241 gcgattaaag aactcaatta cgcgccatca gccctggcgc gtagcctcaa actcaatcaa
    15301 acacatacca ttggcatgtt gatcactgcc agtaccaatc ctttctattc agaactggtg
    15361 cgtggcgttg aacgcagctg cttcgaacgc ggttatagtc tcgtcctttg caataccgaa
    15421 ggcgatgaac agcggatgaa tcgcaatctg gaaacgctga tgcaaaaacg cgttgatggc
    15481 ttgctgttac tgtgcaccga aacgcatcaa ccttcgcgtg aaatcatgca acgttatccg
    15541 acagtgccta ctgtgatgat ggactgggct ccgttcgatg gcgacagcga tcttattcag
    15601 gataactcgt tgctgggcgg agacttagca acgcaatatc tgatcgataa aggtcatacc
    15661 cgtatcgcct gtattaccgg tccgctggat aaaactccgg cgcgcctgcg gttggaaggt
    15721 tatcgggcgg cgatgaaacg tgcgggtctc aacattcctg atggctatga agtcactggt
    15781 gattttgaat ttaacggcgg gtttgacgcc atgcgccaac tgttatcaca tccgctgcgt
    15841 cctcaggccg tctttaccgg aaatgacgct atggctgttg gcgtttacca ggcgttatat
    15901 caggcagagt tacaggttcc gcaggatatc gcggtgattg gctatgacga tatcgaactg
    15961 gcaagcttta tgacgccacc attaaccact atccaccaac cgaaagatga actgggggag
    16021 ctggcgattg atgtactcat ccatcggata acccagccga cccttcagca acaacgatta
    16081 caacttactc cgattctgat ggaacgcggt tcggcttaga tttacgctgt cttttgatca
    16141 aattattacc atcggttgtt ttcagaagca tgaacattgc tgctgaagca acagtaataa
    16201 tgcccatcgt gataaacgta tagtggaatt gttcgacagt cgttgtgcct tccatccctt
    16261 cataaacgcg aaggacggcc gcacttacag caacgcctaa actaatcgac agttgctgcg
    16321 tgaccgccag aacactgtta ccgctgctgg cgttgtcatc ggtcagatcg gcaagtgtga
    16381 tggtattcat cgcggtaaat tgcgtcgaca tagccatccc taatataaac aacggcaaga
    16441 tcagcatcca tatcgccatt gccggtgatt gcaaagagaa ctgagcgatc attagcccaa
    16501 taatcaccgt gatccccact aacgtatggc gatagcccag acgacgtaag acttgggtaa
    16561 ccatcgattt tgcaataatg gaacctaacg ctgtcggtgc catcatacag ccggcaataa
    16621 acgcctgata accaaatcct acctgtaaca tcaatggcat aaggaacggt acacaaccgg
    16681 tccccagacg ggttgcaata ttgcctacga taccgatcga gaaagtgcgg gttttaaata
    16741 aatctaatga aattaatggg tttggcgtgt gtcgcgcatg gagaatatag agaagcagta
    16801 acccgatgct ggtgacaatt accgtcaagg caatccagct ggcgacaatc ttttccccga
    16861 atagctctat tccgcttgag aagagaacaa ggctgaggcc aaacagcaaa aagccagtga
    16921 tatcgaatct gcgtcgtgcg gtggtgaaat tgggcatatg tttgcgcgcg taaagaaggc
    16981 ccgcaatacc tatggggata ttgattaaaa atatccagtg ccaggttgcc caggtgacca
    17041 gcacgccgcc aagaacgggg cctaaaattg gccccaccag acccggcatg gcgacaaaat
    17101 tcaatactgg aagaagttca ttacgaggat aagcgcgcag taaggccagc cgagcaacag
    17161 gcatcatcat tgcgccgcct atcccctgaa taacccggaa gacaaccagc tgtggtagcg
    17221 aattagaaag tgcgcaggcc agagaaccca atgtgaacag actcacggca agggtaaaaa
    17281 tgcgacgcgt accgaagcga tcggctagcc atccgcttac cggaataagc atcgccaccg
    17341 tcagcgtata actgatgatg gctgattgca tcgcgagagg agaacgatta aggctatgag
    17401 cgattgcggg taaggcggta ttcagaatag tggcatcaag tgcctgcatg aagaaggcca
    17461 tcgccgcgat ccacggcaaa cccgccatac tgcgattctt tttatcgctc attcaatgtc
    17521 ctgttatcgg gttgtcactt atcaggtgag cgtagcagcg cctgacaagc tttaaatgcc
    17581 gcgtcgccat cgctttggac aatcgcatcg acaatcgcct ggtgcagatc cagctttatc
    17641 actgtgtcgc tggtaattga cgtgaagtaa gtgtgataaa ccgaatggaa tagcgaggcg
    17701 aatgaggtca aaaacggatt ggcgctcatt tcatagatat gctcatgcca ggccatatcg
    17761 acttcgatcc agcgttcacg gcgaaagttc tcttttaatg ccgccatttc ggccattaac
    17821 gtattgagat gcgccttctg ttccgcggtg ccaaccgttg cagccagtag gcaagcttgc
    17881 ggctccagac agatacgcat aaccagaaag tgatcgatga cctgatgaaa gttctcttct
    17941 gtcatccacc aggtaagcaa ttcctgatca agaaaattcc agtttgattg tggcatgacc
    18001 cgggtaccaa ttcgcggtcg cggtaaaacc atcccttttg ccgttaacgt tttgaccgct
    18061 tcgcgtaccg ctgtacgact cactccaaat tgctcgccca gctcaatctc accaggcaaa
    18121 atggtgccgg gttcatattc accttttaag atgcgttgcg ccagcttctc agccagaaca
    18181 tacgaaaggt ttttctgtgc agctaactgt tgtgcgctta aaggcattac taatcttcct
    18241 ttttcttttt attcctcctt agtatgccac caggaagtgt gattacggtt gcaaaaacgg
    18301 caaattgctt gttttatggc acattaacgg ggattttgct
//
LOCUS       DAZGVQ010000100        18234 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000100 DAZGVQ010000000
VERSION     DAZGVQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18234
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.101"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(32..1891)
                     /gene="gss"
                     /locus_tag="ACHLAG_003334"
     CDS             complement(32..1891)
                     /gene="gss"
                     /locus_tag="ACHLAG_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFP0656501.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2096..2962
                     /gene="yghU"
                     /locus_tag="ACHLAG_003335"
     CDS             2096..2962
                     /gene="yghU"
                     /locus_tag="ACHLAG_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFP0656502.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(2991..3072)
                     /locus_tag="ACHLAG_003336"
                     /pseudo
     CDS             complement(2991..3072)
                     /locus_tag="ACHLAG_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3085..3333)
                     /gene="hybG"
                     /locus_tag="ACHLAG_003337"
     CDS             complement(3085..3333)
                     /gene="hybG"
                     /locus_tag="ACHLAG_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFP0656503.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3346..3687)
                     /gene="hypA"
                     /locus_tag="ACHLAG_003338"
     CDS             complement(3346..3687)
                     /gene="hypA"
                     /locus_tag="ACHLAG_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP0656504.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3680..4168)
                     /gene="hybE"
                     /locus_tag="ACHLAG_003339"
     CDS             complement(3680..4168)
                     /gene="hybE"
                     /locus_tag="ACHLAG_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFP0656505.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4161..4655)
                     /gene="hybD"
                     /locus_tag="ACHLAG_003340"
     CDS             complement(4161..4655)
                     /gene="hybD"
                     /locus_tag="ACHLAG_003340"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFP0656506.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4655..6358)
                     /gene="hybC"
                     /locus_tag="ACHLAG_003341"
     CDS             complement(4655..6358)
                     /gene="hybC"
                     /locus_tag="ACHLAG_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFP0656507.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6355..7533)
                     /gene="hybB"
                     /locus_tag="ACHLAG_003342"
     CDS             complement(6355..7533)
                     /gene="hybB"
                     /locus_tag="ACHLAG_003342"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFP0656508.1"
                     /translation="MSHDPKPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7523..8509)
                     /gene="hybA"
                     /locus_tag="ACHLAG_003343"
     CDS             complement(7523..8509)
                     /gene="hybA"
                     /locus_tag="ACHLAG_003343"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFP0656509.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8512..9630)
                     /gene="hybO"
                     /locus_tag="ACHLAG_003344"
     CDS             complement(8512..9630)
                     /gene="hybO"
                     /locus_tag="ACHLAG_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFP0656510.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9819..10106)
                     /gene="yghW"
                     /locus_tag="ACHLAG_003345"
     CDS             complement(9819..10106)
                     /gene="yghW"
                     /locus_tag="ACHLAG_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFP0656511.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10225..11112)
                     /gene="yghX"
                     /locus_tag="ACHLAG_003346"
     CDS             complement(10225..11112)
                     /gene="yghX"
                     /locus_tag="ACHLAG_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFP0656512.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPARMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11269..12309
                     /gene="gpr"
                     /locus_tag="ACHLAG_003347"
     CDS             11269..12309
                     /gene="gpr"
                     /locus_tag="ACHLAG_003347"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFP0656513.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNNLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12349..12843)
                     /gene="yqhA"
                     /locus_tag="ACHLAG_003348"
     CDS             complement(12349..12843)
                     /gene="yqhA"
                     /locus_tag="ACHLAG_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFP0656514.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13034..13918
                     /gene="yghA"
                     /locus_tag="ACHLAG_003349"
     CDS             13034..13918
                     /gene="yghA"
                     /locus_tag="ACHLAG_003349"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP0656515.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(13957..14382)
                     /gene="exbD"
                     /locus_tag="ACHLAG_003350"
     CDS             complement(13957..14382)
                     /gene="exbD"
                     /locus_tag="ACHLAG_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFP0656516.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14389..15123)
                     /gene="exbB"
                     /locus_tag="ACHLAG_003351"
     CDS             complement(14389..15123)
                     /gene="exbB"
                     /locus_tag="ACHLAG_003351"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP0656517.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15375..16562
                     /gene="metC"
                     /locus_tag="ACHLAG_003352"
     CDS             15375..16562
                     /gene="metC"
                     /locus_tag="ACHLAG_003352"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFP0656518.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16702..17361
                     /gene="yghB"
                     /locus_tag="ACHLAG_003353"
     CDS             16702..17361
                     /gene="yghB"
                     /locus_tag="ACHLAG_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFP0656519.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17401..>18234)
                     /gene="yqhC"
                     /locus_tag="ACHLAG_003354"
     CDS             complement(17401..>18234)
                     /gene="yqhC"
                     /locus_tag="ACHLAG_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFP0656520.1"
                     /translation="SLSELLPDVRLLYGETPFARTPVMYEPGIIILFSGHKIGYINER
                     VFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLNVDILQLQELLMDIGEDEHFQPS
                     MAASGINSATLSEEILCAAERLLDVMERPLDARILGKQIIREILYYVLTGPCGGALLA
                     LVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEANMSVSAFHHNFKSVTSTSPLQYL
                     KNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSREFKRYFGVTPGEDAARMRAMQG
                     N"
BASE COUNT         4431 a         4928 c         4584 g         4291 t
ORIGIN      
        1 cataatcagt acggggttgc tacaacatgt attacttttt caccacaatt aacggttcaa
       61 tatcactctc ttttttgatg accagtgatt catcaccgcg caaacacgtc ccaccgtagt
      121 tgccgcccac ggtgaaggta catacctgaa tgtatttacc gtccactttc ggcaaacacc
      181 acagttgctg atagatgttt ttctgctcgg caaatttacc gctggttttg tccagcacct
      241 cttcatgatg gctgaccagg tcgatattgc taccacagcg accggcaatc ggtttcactg
      301 cgtaacctgt tttcaccaat tcatcattaa cagtgaaatc ggtatccagc aggtaacgat
      361 ggtgcgggaa cagcgaccag aggatcggca gaatcgcttt gttgccgggg atcaccgtcc
      421 acagcggctc aaagactagc acttccgggc gcagcaatac gtcgataaga cgcacttcgt
      481 tttgcggatg accggtacgg attggcaccg cagcaaactc acggtcgcta acttcacgaa
      541 tctgatcaaa cgcggtttcc cacgcccagg ttttccacac gcagttaacc agtcgccctt
      601 ccccatcaat cagttgcccg gcagcatccc agcccagttc atccaatcca cgcaagatac
      661 gcgtttcaaa gcccgcctgg tgcagcgcct gctccataaa ctgcgcgtga tagttttcct
      721 cgatatcttt gtcctgcatg atatggacaa acggacgtgc acgactgtgt ttccaggcac
      781 cagccaattc gttaatcagc ccttccgccg gattgaagcc gttgccttta tagccctgct
      841 ccgcccaacg ttcgaggatc aagcccgctt cggtatgaca ggaggcggag tcggcgttgt
      901 actcgtaaac cttcaggcca cgctcatcca tgcagaaatc catacgacca gtgatcatat
      961 ggtgacggcg acgctgccag gagagacgca aacgtggcca gaggattttc gggatgtcga
     1021 acagcgccag caggttgtca tctttcagca ccttgtcggt tgcgtgaaga tacatcaggt
     1081 gcagctcgtt ggtggcttta attagctcct gctcggcact ctcggtaatg gtgtagtaat
     1141 gataaggatc ctgattgatc acctgaccgt tggcctgcac ataggcgttt tgcagcggat
     1201 ctttttcatc cagccattta ccgtcaaact ggcctttgtt ttccaggcgc gctccgctga
     1261 ttttcagcag ctcgcctgca atttccggct gcggtaagct gtattcagta tcttccgtct
     1321 ggatcatcca gcccagaatg gtggtgtcat caaaagtgtc tttcagggta tagcagccgt
     1381 tttcgaccac catctccagc tcacgcgtcc actgttgccc ttgcggcaac ggggaatgaa
     1441 tcacgttctg ttccgcaata cggactttgt tgccatgcaa ttgggtaatg atggcgacat
     1501 ggccagtgtc tttaaattca ccgcctttat cccagataag aagcgcaccc gcgaccggcg
     1561 cacgcggcga gccgttagga aatgcctgca atggcaggat gttgtcatta accacttctc
     1621 gcaggaagcg cagcgagaaa atctcccacg ccatacccac gtcagtaaag accacaccgt
     1681 aattcagaaa gagaaaacgg cgagcaaatt caacgcattg ccacttgtgg cccatatatt
     1741 cgtcgtcgat atagctacgg aatacggcgt catcttcgta ttcctgcgga tcgagagaac
     1801 tgtaatctga agagtagatt gctaccccac ctggggcgta gcccaataat gtcccgaacg
     1861 gggcatcctg gctggtcgtt cctttgctca tcactttacc tttaacaata caacctaagc
     1921 agttggcgta attgttgtgc tctgattacc tgctcagcga cattaaccgg acagaggtca
     1981 gactaatcat acacctcatc gcagcggctg gcgcaaaggg ttaaaaaatt cacattctgt
     2041 acagcaagtg acctgctaca ctgcttcaac actaccactc agaaggcaac tcactatgac
     2101 agacaatact tatcagcccg cgaaagtctg gacgtgggat aaatccgctg gcggcgcgtt
     2161 cgccaatatc aatcgcccgg tttctggtcc gacgcatgaa aaaacgctgc ccgttggcaa
     2221 acacccattg caactttatt cgctgggaac gccgaacggt cagaaagtaa cgattatgct
     2281 tgaggagctg ctggcgctgg gcgttactgg tgcagagtac gacgcctggc tgattcgtat
     2341 tggcgatggc gatcaattct ccagcggctt tgtcgaagtg aacccaaact cgaagatccc
     2401 ggcgctgcgc gatcatacgc ataatccgcc gatccgcgtg tttgaatctg gttcgatcct
     2461 gctttatctg gctgagaaat ttggctactt cctgccgcag gatttggcaa agcgtactga
     2521 aacgatgaac tggctgttct ggttacaggg cgcggcaccg ttcctcggcg gtggttttgg
     2581 tcacttttac cattacgcgc cgatgaaaat tgagtacgcc atcaaccgct ttaccatgga
     2641 agccaaacgt ctgctcgacg tgctggataa gcaactggcg cagcataagt ttattgcggg
     2701 cgatgagtac accattgcgg atatggcgat ttggccgtgg tttggcaatg tggtgttagg
     2761 cggcgtgtat gatgccgcag agtttcttga tgcgggcagt tataagcatg tacaacgctg
     2821 ggcgaaagaa gtaggcgaac gtccggcggt gaaacgtggg cgtattgtta accgcaccaa
     2881 cggaccgctg aatgagcagt tgcatgagcg ccatgacgcc agtgatttcg agacgaatac
     2941 ggaagataag cgtcaggggt aagggttggt gttcgtcgca gcaagccatc caggccggat
     3001 aaggcgttca cgccgcatcc ggcaatcgtg cacaatgcct gatgcgacgc tgccgcgtct
     3061 tatcaggcct acgaaagcta atcatcacgc gctggtaatg tcgtaatcca tttggcgcag
     3121 tgcgtctaat gtggctttgg cttcatcttc gtcgatgatg ctcatggcaa atccgacgtg
     3181 taccagcacc cactggccca gcagatcggc agggttacct tcacaaatca gggcgatatt
     3241 cacgtcgcgc ttgataccac atacttcaac ctgcgcaagc tggtgaatat cttcaccgac
     3301 agccagcacc tggcctggaa cgccaataca cattattaac tccggttatt caacttcaat
     3361 acttttgacg atcagcgaat cgccggtatc gacacgcaac cgctcgccat gacagatcgg
     3421 acactgcgca tcgtgctgat gaatctccac cacctggctg caatcccagc accaggcctg
     3481 ggcgggttta tagacgatat gtaaatcgca cccttgcgcc accgttccgt ggcagacaat
     3541 ttcaaaacta aaacggacgg cgctctcctc aacgcaggag agcgcgccaa tttccagcca
     3601 cacggcggtg acgcgcttaa catcgtgctg ctccgcctgc cgttggataa tttcaacggc
     3661 gctctggcaa agcgacaact catgcatttt cgccactcct gcgaccaaac agcagggcgc
     3721 gacgccctgc gtgtggtaca tccggattcg tgactggcag cgaaaggatc attcgtgcgc
     3781 agtcatccgt caggcgttgg ccctcttcaa tcgacatact gtgcgaaagc ggtgacatca
     3841 gcgaacagga gaggtattgc gaaacaccgt ccagttcgcc aacggtaaag gtcatggtgc
     3901 catacggcag ttgcagacca attttttcac tcactttgcg cagcggccag agttgatccg
     3961 ggccggggaa gataactgca ctcagcatcc acggggtgat cacacacccc gtccactgac
     4021 cttcgaacag cgtaaaatca gaaacataca ccggcattga aggatgcaga aaagagagat
     4081 cgtgcatcga acgccgggca atttcttcaa acgctgcctg tacttgcgcc ttcggggagg
     4141 tctggaaacc tgctatctct tcagtcatga atcgcctccc gtgggatggc ttccacgcct
     4201 gattcgcgca gcgcagccag aacctgctca agcgcaggtt caatcattgc ttcaaccgtc
     4261 ggcgttaagc cgatgtgtgg ctccagcgat tccgggatca cgccgaccag ggtcagcttt
     4321 ttcggaaact cgccggtgaa gcgcagggcc gacaagacgt cggccaggcc aagctgatgc
     4381 ggagagattt tgttggtaaa caacgccgga acttcttcat cccgcaggat catcatcgtt
     4441 cccggcgcgc tcttcttcga cacaatggca tccgcaataa tcagatgatc gcgatttgcc
     4501 atgtcgccaa gcagctccat tcccgccgtg ccgccatcga ggatctcaac ataatccggc
     4561 agaatgtatc gttgctctaa cgcttcaaca atccgcacac cgatggcttc atcggtcagc
     4621 aaaatattgc cgacccctaa gactaaaata cgcattacag aaccttcact gaaaccactt
     4681 cgttgccgtc ggcatccact acgtgtaccg cacaggccat gcacgggtcg aaggagtgaa
     4741 tggtacgcac cacttccagc ggtttattcg gatcagcaac cggtgtaccc accagcgact
     4801 gctcgtaagg accgacgtca tcattgaagt tacgcggacc agagttccag gttgatggaa
     4861 caactgcctg gtagttgctg atgataccgt ctttgatcac catccagtga gagagcatac
     4921 cgcgcggcgc ttcgaggaag ccaacacctt tgaactcacc cgttgccgga atgttcggtt
     4981 tcacgaaggt ggtgtgatcg cctttgccga tattggtgat cagtgcactg tattggtttt
     5041 gcaggatatc ctgcaattcg cagcagtgaa cggtacgacc aataatacgg cccagcgtag
     5101 agtgcagttg cgccacttcc agcgtgttac cagtcagttt ctgataaatc gcaacgattt
     5161 cattcagttt gttctgggta gattctcgac ctgccgccag tttcaccagc atattagcca
     5221 gcggacccac ttctaccgtt ttgccgtaga aagtcggtga tttcacccag gaatatttcc
     5281 cgtcgtcaga ccaaccatca taagccggaa tggtggtacc ttcccacggc gcctgtggcg
     5341 cttcgtcttt ataccaggag tgcttcgcgc tttcctgaat gcctttgatc aggtattcat
     5401 cggaatgaga agtgatcgga cgatacgagg acagatccgc attctcaatg tagccgcccg
     5461 ggaacaggaa gctgccgttt ttgctgtcgg tcgggaattc cggcacgctc aggtagttca
     5521 ccgcaccttt accgcgcgtc agccattccg ggtagaacgc ggcgataacc gcggtatcaa
     5581 ccttataaac ctgctcaaca aagtcgctca gtttgtcgat gaaagacttg atgtacatca
     5641 agcgctcaag gttcaacacg cccaacccgt cgaggttgat tgggttcgcg acaccaccca
     5701 ctgccaggtt ctgaatgtgc ggcgttttac cgcccagcag cgccacgacg cggttagcgt
     5761 cacgctggca ctccagcgcc tgcaggtagt gcgctaccgc aatcaggttc acttccggcg
     5821 gcagtttcat cgctgggtga ccccagtagc cattagcgaa aatacccaac tgaccgctgg
     5881 caaccagatc tttgatcttg ttctgaactt tggtgaactc ttccgggctg ttcagatgcc
     5941 aggtcgaaac gcctttcagc atttcggagg ctttggttgg gtcagcttgc agtgcagaag
     6001 tgatatccac ccagtccagc gccgaaagct gatagaaatg aacgatatgg tcatgcgtgg
     6061 tgtgcgcagc cagaatgatg ttacggatgt attgcgcgtt aaccggaacg tcgatattca
     6121 gcgcactttc tgccgcacga acggaagaca gcgcgtgagt ggtagtacat acgccacaga
     6181 tacgttgcac aatcatccat gcatcgcgcg gatcgcggtt tttcacgatc tcttccatgc
     6241 cgcgccacat ggtaccggaa gcccatgctt tcgaaacgac gccattttcg atttcgcaat
     6301 cgatgcgtaa atgcccctca atacgggtta ccggatcaat agtaattctc tggctcatgc
     6361 tttgctcgcc tcatgacgat tatgatcgtt ttgttttaaa ggaggaagta tcggcagtag
     6421 acgaatgagt acgatgtaag cgcaaatctc aatagccaca aaaccaatag aaatcaacag
     6481 ttcttcccag gtcgggaagt aggcgtaacc gccgcccggg ttgaatgcca ccagcgaata
     6541 ggtcagacgc caggttgcac aacctaacag tgcgctcagt gctgacagga acagcatgcg
     6601 ggaatcatta cgcagcttcg ccacacgcag aacgaccagc gggaagagca tcagcaggac
     6661 ttcaatccag aacatcacgg agtagaagtc accggcaaac gctaacgaca gcttgtcgcg
     6721 atagataagc tcgccaaagc gcagcacgat gaaaatcgcc agcaacacac tgatggtgtt
     6781 ggtcagcttg acgaacaggc tcttttcatc cggaccgttg ccacgcagac ccgcctgcac
     6841 cagtgaacct tcaaagatga caatcgagaa gcccatgatg aacgccgtca gcagcgagaa
     6901 cagcggcaac atttcatagc tctgccacag cggatgcacc ttgtagcccg ccgagatcat
     6961 cagcgacccc attgaagact ggtgcatggt cggcagcaac gcaccgagcg cgatgatgaa
     7021 gaacatcacc ttgttcagac gctgtagcga caccttccag cccagacgtt caaacagtgc
     7081 cggagcaaac tccagagcca tcacgccgat gtagatggtc atacagaccg ccgtctcgaa
     7141 cagtaccgag ttcacgttga agtgacccgg aatgtagaag tacggcaggt tccagtagcg
     7201 acccacgtcg atagtgatcg acaagccacc cagtgagtaa ccaaacagac tcgccaacag
     7261 cgccggacgc accagcggat ggtattgccc acggttaaag acgtataccg cccacgccag
     7321 cgcccagccg ccacaggcaa agccagtgcc gatcagcagg tcaaacgcga tccacacgcc
     7381 ccacggaaag ccgccgttca ggtcggagac agagcccaga ccgaacacca gacgcttcac
     7441 aatcaggagc atacagatga cgattaacgg tccaaaaatc atgaccggtt tactgatgat
     7501 tttgccgccc agcggttttg gatcatgact catgatcgtc tcctccgtcg tgatggtcgt
     7561 ttttggtgtt gcgacgaacc agcacggtta agcccgccag cacagccagt ggtagcatca
     7621 tgcctttata cagggtgtgt tgaatatttt cggaacgcgc accggtagaa agatcgtcca
     7681 gtttcggcag gtcgagattt tcataaggca cacccgtcag taccagaacc tgagtaccgc
     7741 cgccctcttt ctcgccgtac agatgcggat aatatttcgg caccgtatgc aggtaagtgt
     7801 cgccagattt cagcgtctga cgcggatagt ggtattcgct gccaggcttc agcgccagac
     7861 gttttttcgc ctccgccatc agctcttcac gcgtaccgaa aatcaccgcg cccgccgggc
     7921 acacttctac gcagccaggc agaccgcctt tatcgagacg ttccacacct ttctggttgc
     7981 acagctcgca cttatgcagc gcaccaaacg ggttgttgta gtcgtacttc ggcacgttgt
     8041 acggacaggc gaccatgcag taacggcaac cggtgcacac gtctttgtcg taatggacaa
     8101 tgccggtttt cggatctttt ttcagtgcag agaccgggca cacagagaca cagttcggat
     8161 cgacgcagtg catacactgt ttcttaatgt acgcgtagcc gttctcctcc tggtctttgt
     8221 tgacccctgt gccgctggtc cacacctgaa tgatgttatt ggtatacggc gacagtttgt
     8281 cgttgttcga ccaggtctgt tccccttgcg ggttacgttc agggaaattg atatcctgac
     8341 acttggtgac gcaagcctgg cagcctacgc acaaggtcga gtcgtacaac atccccagcg
     8401 atcccggaat tggcgggcgg ttttcagcag ccgcatgact gacagacggc agcgcgcccg
     8461 tcagcaatgc cccgcaggag gctgctttaa taaaattacg tctgttcacg gttattctcc
     8521 ccgtgagtca gcgttatctt tcttttgctg acgacccagt tcacgcaccg ccatcacgct
     8581 gacaccggca accaacccaa ccacaccgcc gagcaaacca atagcgcctg cagagacgtt
     8641 gccgccctct ttagcgttaa catccggttt ctgtgaacgc ggagtttgat tttcgacgtt
     8701 ggcaagctga tggatgcctt tatggaagcc gataccttct tcgttacagc catagcacgg
     8761 gtgaccaatc gccaccggcc acacaccgcc aacatcgcag aattgcagcg ttgagcagtt
     8821 gccgtaagtt tctggccctt tacagccgag gtggtacagg caccagcctt cgcggtggcc
     8881 ttcatcaccg aactctttgg caaaacgacc agcatcgaag tgcgggcgac gttcgcagtg
     8941 ttcgtgaatc agacggccat aggcgaaggt cggacggttt ttgtcatcca gtttcggcgg
     9001 tttgccgtaa gtgatgatgt gcgcaacggt cgcgaggaag ttgtgcgggt tcggcgggca
     9061 gcccggaata ttgataacgg ttttgcctgg cagaacttct tgcaggctga ctgcgccagt
     9121 tgggttaact ccagctgcgg caacaccgcc ccacgcagag caggaaccga tagcgataat
     9181 ggctgctgcg ccttccgccg ctttgcggat gtgatccaca ataggctcac cggcaaccat
     9241 gcaataaata ccgttatctt ttaatgggat ggaaccatcc accactaaca catactgccc
     9301 tttgtacttc tcgagagcgt tatgtttgtt ctcttcgacc tgatgaccga aggcggcgga
     9361 aagcacttcg tgatactcca gagagatagt ctccagtacg aggttttcta ccgttggatg
     9421 cgttgcacga agcagagatt ccgtacaacc ggtgcactcc tgcgcgccaa tccagataac
     9481 tggcggacgc tgcgggttag taaccgattc ggccatctct gcagcggctt tgctacttaa
     9541 ccccatggtg gcggctaatg ctgcacaaag cttcatgaaa tcacgacggt taatgccgtg
     9601 agaatggatg agggtgttat ctccagtcat ttatagttat tccgttgcga agacctggca
     9661 tatattttgc ctcaatcgca aaatcaataa tgcgatcgat gcgccattta ccacacattt
     9721 attatggtta tcgatatcat gatactgcgg cggggcgcta aaacgaaggg aagaagcatt
     9781 agtgtaatta attaaagcaa gataatacgt atgtttgatc aattttcgag gcgataaccg
     9841 gccataaaaa agcgcaacgt agaacaggaa ttattctcac gaaagaaatc cattaccatc
     9901 tctttatcca gtccatagcg gcgcgtcaga atacccgctt cccaggcgct aagctggcga
     9961 tctccggtct tttcgtacat ccggtgtgag tagcctaata caaaaccgcg tttataatcg
    10021 gcacagaatt ttgtcacatt aaccgcactg tcggcatgcg ttgcttttaa tcccgccatt
    10081 aagcctttac caaaatggtt attcatcgtt cacctcattc gctatatatt gtcatatata
    10141 gcgatatttt aggcaaagaa cgacttttgc gcacgctatt ttttcatgct tgtttaagac
    10201 gtaatcccgt aagagataaa cctatcagga gagatattta tcgaaccatt tcagtgtcct
    10261 ttgccaggca agatcggcgg cagatttgtc ataacggggc gtggaatcat tatggaatcc
    10321 gtgattaacc cccggataga tatacgcctc ataaacctta ttattggctt tcaacgccgc
    10381 ctcgtaagca ggccagccct cgttgattcg ggtgtccagt tccgcgaagt ggagtagtaa
    10441 aggcgcttca atcttcgcca catcggcagt gggtgcctga cgaccataaa acggcaccgc
    10501 gcaggccagt tccggatacg cgacagccgc cgcgttcgat acaccaccgc catagcaaaa
    10561 tccggtaata cccactttgc ctgccgcttg cggatagcgt tgcataaact caattgcggc
    10621 aaagaaatca ttcatcagtt tggttggatc aacctgctgt tgcagctcac gacctttatc
    10681 atcattaccc ggataacctc caacggaact taagccgtca ggtgccaggg cgatataccc
    10741 cgccttcgcc actcgccgtg ccacatcttc gatatacgga ttcagtccac gattctcatg
    10801 caccaccacc acggctggcg ttttgccgct catccttgcg ggtttcacca gataaccccg
    10861 tacctcgccg tgaccatttg gcgaaggata cgtgatgtac tcagcaacaa tctccgggtc
    10921 ggtaaactct acctgagtcg ccagcgcata atttggcttg agcaaatcaa acaacgctaa
    10981 tgccgtcatc ccgcctaccg catacttcgc cgcaaggttg aggaactcac gtttcgagat
    11041 tttcccgtga gcgtaatagt cgtagtaatc caacaactct tgtgggaaat ctttggcggt
    11101 taaacgcggc atcgttgcac tcctcaactg gtgttttttt aagcaaagca taagcacgtg
    11161 ttttcgccca gtttttcgtc actctgtgag ccagactacg ggatacgcgc tggcgaatcg
    11221 ctaaactaga aacattgttt cgaaattgaa cggtggaaag gagaggtcat ggtctggtta
    11281 gcgaatcccg aacgttacgg gcagatgcag taccgctatt gcggaaaaag cggtttacgc
    11341 ctgcccgcgt tatcgctcgg tttatggcac aatttcggtc acgttaacgc gctggaatca
    11401 cagcgtgcga tcctgcgtaa agcgtttgat ttgggcatta cgcactttga tttagccaac
    11461 aattacgggc cgcctccagg aagcgcagaa gagaactttg gtcgcctgct gcgggaggat
    11521 tttgccgctt atcgcgatga actgattatc tctaccaagg ctggctacga tatgtggccc
    11581 ggcccgtacg gctctggcgg ttcacgtaaa tacctgctcg ccagcctcga ccaaagcctg
    11641 aagcgtatgg ggcttgagta tgtcgatatc ttttactctc atcgcgtcga tgaaaatacg
    11701 ccgatggaag aaaccgcctc tgcgctggct catgcggtac aaagcggtaa ggcgctgtat
    11761 gtcgggatct cctcttactc gccagagcgg acgcaaaaaa tggtcgagtt gctgcacgag
    11821 tggaaaattc cgcttttaat tcatcaacct tcgtacaatt tactgaaccg ctgggtggat
    11881 aaaagcggcc tgctggatac cctgcaaaat aacggcgtgg gctgtattgc ctttactcct
    11941 ctggctcagg gattgctgac cggaaaatat ctcaacggca ttccgcaaga ttcacggatg
    12001 catcgtgaag ggaataaagt tcgtggtctg acgccgaaaa tgcttaccga agccaacctc
    12061 aacaacctgc gcttattgaa tgaaatggca cagcagcgtg gacaatcaat ggcgcaaatg
    12121 gcgttaagct ggttgctgaa agatgatcgc gtgacgtcgg tattgattgg tgccagccgc
    12181 gcggagcaac tagaggagaa cgtgcaggcg ctgaataatc tgacatttag caccgaggag
    12241 ctggcgcaga ttgatcagca tatcgccgat ggcgagctga atctgtggca ggcgtcgtcc
    12301 gataaatgac ctgttaataa cgggcgcgcg aaccgcgccc gaatcagatc agtgattatg
    12361 acgagtcagt cggtcaagat agcccatcac aaatgcagaa agcacaaacg tcagatggat
    12421 aatgacgtac cacatcagtt tgttatccgg gacatttttc gcatccataa agacgcgcag
    12481 taggtgaatg gaagaaattg ccacaatcga cgctgctact ttgtttttca gcgacgttgc
    12541 gtccattttc cccagccagt tcagcttctc tttgttctcg gagatatcca gctgtgagac
    12601 gaaattctca taaccggaaa acatcaccat caccagcaaa ccgccaacca gcgtcatatc
    12661 caccagcgac agcaacacga ggatcaaatc tgattccgcc atcgagaaga tattcggcag
    12721 tacgtgaata atctcctgga agaacttcag cgccagggca actaacgcca gcgaaaggcc
    12781 aaagtacacg ggggcaagca gccagcgaga agcatacatt gcattttcaa gaaaacgttc
    12841 catacattcc tgtcgtgtag ttaaaacggg caacagtata tcgcaattga gtaaacagca
    12901 cgcaacagca aatcaacata tacgttaaac ctttgtcgca taattctgag gtttagggat
    12961 cttcctggtt cccttatcaa cgcatcgaga acgtctaccc ttaaaggacg ttgaacctga
    13021 agggagaaaa acgatgtctc atttaaaaga cccgaccacg cagtattaca ctggtgaata
    13081 tcccaaacag aaacaaccga cgccaggcat ccaggcgaag atgacaccgg taccggattg
    13141 cggcgagaaa acctatgttg gtagcggtcg cctgaaagat cgtaaagcac tggtgacagg
    13201 gggcgattcc ggaataggtc gcgctgccgc catcgcttac gcgcgtgaag gggctgacgt
    13261 ggcgatcagt tatcttcccg tggaagaaga agacgctcag gatgtgaaaa agatcattga
    13321 agaatgcgga cgcaaagccg ttctgctgcc aggcgattta agcgatgaga aattcgcccg
    13381 ttcgctggtt cacgaagcgc ataaggcgtt aggcgggctg gatattatgg cgctggtcgc
    13441 cgggaaacag gttgccattc ctgatattgc agacctcacc agcgaacagt ttcaaaagac
    13501 ctttgccatt aacgttttcg cgctattctg gctaacccag gaagcgatcc ccctgctacc
    13561 gaaaggtgca agtatcatca ccacttcgtc aatccaggca taccagccaa gtccgcattt
    13621 actggactat gcggctacga aggcggcgat tctgaactac agccgtggcc tggcaaaaca
    13681 ggtcgcggag aaaggtattc gggtgaatat tgtcgcgcca ggcccgatct ggacagcact
    13741 gcaaatttcc ggcggacaaa cgcaggataa gatcccgcag tttggtcagc aaacgccgat
    13801 gaagcgtgcg gggcaaccgg cggaactggc ccctgtatat gtttatctgg caagtcagga
    13861 gtcgagctac gtcaccgcag aagtgcacgg tgtgtgcggc ggcgagcatt taggttaaaa
    13921 aaatgcccgg ttgtgaaaag caaccgggca tcatttttat ttcgctttgg cggtttcttc
    13981 gccgaccaga cctatcttca ggtaacccgc ctgatgcagc gtatccatta ccttcatcaa
    14041 cgtctcgtaa tcgacggttt tatccgctcg gaagaagatg gtggtgtctt tcttgccttc
    14101 ggttaacgca ttcaacgccg taatcattgt ttcatcggtg accggatcgt taccgataaa
    14161 catcgagttg tctgccttca ccgacagata aaccggtttt tccgggcgcg gctgcggcgt
    14221 gctggtagaa gcaggcaaat tcaccttcac atctaccgtc gctaacggtg ccgccaccat
    14281 aaagataatc agcagaacca acatcacgtc gataaacggc gtcacgttga tatcatgcat
    14341 ttcgccgtta tcatcgaggt tttcgttaag atgcattgcc atcggacatt atcctgcgcg
    14401 taatttttgt gcgacacgaa ccggatgcgc agcggcgctg gcttccagat ccaggtcacg
    14461 gctttgcagc aacaatacct gcgctgcaac atcacccagc atcgctttaa agccgccaat
    14521 ctggcgtgca aatacgttat agataacgac cgcaggaatc gctgccacga ggccgattgc
    14581 cgttgctaac agagcttctg cgatacccgg cgcaacgact gccaggttag tggtctgcgt
    14641 ttgcgcgata ccaataaagc tgttcatgat gccccagacc gtaccaaaca gaccaacgaa
    14701 cggagaaatc gcgccgatgg ttgccagata gccgttaccg cggcccattt gacgacccac
    14761 tgcggcgacc cgacgctcca gacggaagct ggtacgttct ttaatacctt cgttatcgtc
    14821 gctgccttct gacagttcca gctcattctg cgcttcattg agcaaatgca ggcttaagct
    14881 tttgctacca aaatcagcgg cgatatcgtt ggcctggttt aaggaacgcg cttcagccag
    14941 cagttgctgc tcgcgcttaa ggcgacgctt ctgattgaag aactctacgc tcttactgaa
    15001 gaagattgcc caggtgacta cggaggccaa aataagccca atcatcacgc acttaacgac
    15061 aatatcggcg tgctgataca taccccagac ggaaaggtcc gtctgcatta aattattacc
    15121 cactgtgtat ctccaggacg caagtcacaa aatctgcgca taataatatc aaaacgacgt
    15181 cgaattgata gtcattctca ttactatttg catactgccg tacctttgct ttcttttcct
    15241 tgcgtttacg cagtaaaaaa gtcaccagca cgccatttgc gaaaattttc tgctttatgc
    15301 caattcttca ggatgcgccc gcgaatattc atgctagttt agacatccag acgtataaaa
    15361 acaggaatcc cgacatggcg gacaaaaagc ttgatactca actggtgaat gcaggacgca
    15421 gcaaaaaata cactctcggc gcggtaaata gcgtgattca gcgcgcttct tcgctggtct
    15481 ttgacagtgt ggaagccaaa aagcacgcga cgcgcaatcg cgccaatggc gagttgttct
    15541 atggacggcg cggaacgtta acccatttct ccttacaaca agcgatgtgt gaactggaag
    15601 gtggcgcagg ctgcgtgcta tttccctgcg gggcggcagc ggttgctaat tccattcttg
    15661 cttttgtcga acagggcgat catgtgttga tgaccaacac cgcctatgaa ccgagtcagg
    15721 atttctgtag caaaatcctc agcaaactgg gcgtaacgac atcgtggttt gatccgctga
    15781 ttggtgccga tatcgttaag catctgcagc caaacactaa aatcgtgttt ctggaatcgc
    15841 caggctccat caccatggaa gtccacgacg ttccggcgat tgttgccgcc gtgcgcagtg
    15901 tggtcccgga tgccatcatt atgatcgaca acacctgggc agccggtgtg ctgtttaagg
    15961 cgctggattt tggcatcgat gtttccattc aggccgctac caaatatctg gttgggcatt
    16021 cagatgcgat gattggcact gccgtgtgca atgcccgttg ctgggagcag ctgcgggaga
    16081 atgcctatct gatgggccag atggtcgatg ccgataccgc ctatataacc agccgtggcc
    16141 tgcgcacatt aggtgtgcgt ttgcgtcaac atcatgaaag cagtctgaaa gtggctgaat
    16201 ggctggcaga acatccgcaa gttgcgcgag ttaaccaccc tgctctgcct ggcagtaaag
    16261 gccacgaatt ctggaaacga gactttacag gcagcagcgg gctattttcc tttgtgctta
    16321 agaaaaaact cagtaatgaa gagctggcga actatctgga taacttcagt ttattcagca
    16381 tggcctactc gtggggcggg tatgaatcgt tgatcctggc gaatcaacca gaacatatcg
    16441 ccgccattcg cccacaaggc gagatcgatt ttagcgggac cttgattcgc ctgcatattg
    16501 gtctggaaga tgtcgacgat ctgattgccg atctggacgc cggttttgcg cgaattgtat
    16561 aacattgcca cttttggaca attttgcaga catttaattg tgaaaagtct taaattgttg
    16621 cgtccgggat caaggcgtcc cggacgaatc aggagtacaa taggcaaata aaggcttaaa
    16681 cgctgttcca caggaaagtc catggctgtt attcaagata tcatcgctgc gctctggcaa
    16741 cacgactttg ccgcgctggc ggatcctcat attgttagcg ttgtttactt tgtcatgttt
    16801 gccacgctgt ttttagaaaa cggcctgctg cccgcctcat ttttgccagg cgacagcttg
    16861 ttgatactgg caggcgcatt gattgctcag ggggttatgg attttctgcc tacgattgcg
    16921 attctgaccg ccgcagcaag tctgggctgc tggctaagtt atattcaggg gcgctggtta
    16981 gggaatacca aaacggtgaa aggctggctg gcacagcttc ctgctaaata tcaccagcgc
    17041 gccacctgca tgtttgaccg ccacggtctg ctggcgctgc tggctggacg ttttcttgca
    17101 tttgtccgta cgctgctgcc aaccatggcg ggaatttccg gtctgccaaa ccgccgcttc
    17161 cagtttttca actggttaag tggattgctg tgggtcagcg tggtaaccag ttttggctat
    17221 gccttaagta tgattccgtt cgttaaacgc catgaagatc aggtaatgac gttcctgatg
    17281 atcctgccaa ttgccttgtt aaccgctggc ttgttaggca cgctgtttgt ggtgattaaa
    17341 aaaaaatact gtaacgcctg acgattttcc ccgttcccgg ctgctgtacc gggaacgtat
    17401 ttaattcccc tgcatcgccc gcattcttgc cgcatcttcc cccggcgtca caccgaagta
    17461 acgtttaaac tcacggctaa attgcgatgc gctttcatag ccgacgcgca tcgctgctgc
    17521 gctagccttc atgccgtcat ggatgatcat catccgcgcc ttatgcagac ggtaattctt
    17581 caaatactgc aacggcgagg tgctggtgac agacttaaaa ttatggtgga acgccgatac
    17641 gctcatgttg gcttctgccg ccagttgctc gacgctcagg ttttcggtgt atttattctc
    17701 aatccgtttc agcacgcggc taatcagact gaagtgagtc tggcgactga ccagcgccag
    17761 taacgcgccg ccgcaaggtc cggtcagcac gtagtacaga atttcgcgga tgatctgttt
    17821 gccgagaata cgcgcatcca gtggtcgctc catcacgtcg agtaaccgct ccgccgcgca
    17881 taaaatctct tctgataacg tggcggagtt aatcccgctg gctgccatcg acggctggaa
    17941 atgctcatct tcgccaatgt ccatcaacag ttcctgtaac tgcaaaatat cgacattgag
    18001 acgcaaccct gccagcggca cctctgacgt ggcataggtt tcgcactcaa acggcaacgg
    18061 caccgtcagc agcaggtatt cattggcatc ataacgaaac acgcgttcat tgatataacc
    18121 gattttatgc ccggaaaaga gaattatgat gccaggctcg tacatcaccg gtgtacgtgc
    18181 gaaaggcgtc tcgccataca acaaacgcac atcgggcaac agttctgaca aact
//
LOCUS       DAZGVQ010000101        18221 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000101 DAZGVQ010000000
VERSION     DAZGVQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.102"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            262..1209
                     /gene="stxA1a"
                     /locus_tag="ACHLAG_003355"
     CDS             262..1209
                     /gene="stxA1a"
                     /locus_tag="ACHLAG_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFP0656521.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1219..1488
                     /gene="stxB1a"
                     /locus_tag="ACHLAG_003356"
     CDS             1219..1488
                     /gene="stxB1a"
                     /locus_tag="ACHLAG_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFP0656522.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            1993..3933
                     /locus_tag="ACHLAG_003357"
     CDS             1993..3933
                     /locus_tag="ACHLAG_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0656523.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGELPSLAESGNHPHVSAKPEAAPEYYYVIALAGQSNSMSYGEGLPLPET
                     YDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYG
                     TVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDAGGASENSTRWGVD
                     KPLYKDLIGRTKAALKKNPKNVLFTVVWMQGEFDFDGTPGNHAAQFGALVDKFRADLT
                     DMAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDEN
                     GVNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLATAI
                     LRHAGRVALNAGASSTVSEVRPSSPSGAEATGVTTLLSYLASESEGSLKVQGWSASGG
                     RAEVVSDAEGTGGKAVKVTKEAGKSTWVLEYAAGNGAALLQKGGQIRCRFKASGALAA
                     NQYVMAFYWPVSSLPQGVVLTGDGGNNLLAAFYIQTDAKDLNVMYHNAKVATNNLKLG
                     SFGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFSADKLHVTDITK
                     SATYPVLIDSIAVEVNNADAAA"
     gene            4069..4248
                     /locus_tag="ACHLAG_003358"
     CDS             4069..4248
                     /locus_tag="ACHLAG_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0656524.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLV"
     gene            4289..4534
                     /locus_tag="ACHLAG_003359"
     CDS             4289..4534
                     /locus_tag="ACHLAG_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP0656525.1"
                     /translation="MSEITSLVTAEAVKDVLRSEEVLSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            4612..4827
                     /locus_tag="ACHLAG_003360"
     CDS             4612..4827
                     /locus_tag="ACHLAG_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP0656526.1"
                     /translation="MYQMEKITTGLSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            4832..5365
                     /locus_tag="ACHLAG_003361"
     CDS             4832..5365
                     /locus_tag="ACHLAG_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0656527.1"
                     /translation="MSRKFRYGLSAVVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     SAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            5638..6204
                     /locus_tag="ACHLAG_003362"
     CDS             5638..6204
                     /locus_tag="ACHLAG_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFP0656528.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPIVEGMGLAWAPQFTKL
                     KQRFAKGVTEIIIPSAGGEQTMTCLALRKLAAWLQTINSNKVKPEIRDKVIQYQEECD
                     DVLYEYWTKGVVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKAAHVSK
                     IRTLINEANLLIDFVLADTGKGKITKAD"
     gene            6219..6353
                     /locus_tag="ACHLAG_003363"
     CDS             6219..6353
                     /locus_tag="ACHLAG_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656529.1"
                     /translation="MISDKLITLAKILCVIVGISFLVMLVAIFFSTAWRVLTLSGLVG
                     "
     gene            6581..6787
                     /locus_tag="ACHLAG_003364"
     CDS             6581..6787
                     /locus_tag="ACHLAG_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP0656530.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPA
                     SNSLQLLDETFSVSETESSATRQH"
     gene            7009..7506
                     /locus_tag="ACHLAG_003365"
     CDS             7009..7506
                     /locus_tag="ACHLAG_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFP0656531.1"
                     /translation="MKSLTLFNQPIRVAEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            7503..7760
                     /locus_tag="ACHLAG_003366"
     CDS             7503..7760
                     /locus_tag="ACHLAG_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656532.1"
                     /translation="MSDRVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            8160..8966
                     /locus_tag="ACHLAG_003367"
     CDS             8160..8966
                     /locus_tag="ACHLAG_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP0656533.1"
                     /translation="MAKLDWKKLEQAFRREHAETGITLLDWCRKKKINYNTARTRIKM
                     GKIDHEIDHKTDHEIDHDISDEEPCNDAGSGDEKCAKNSEKNCANSAETKRIRGSRLL
                     PPSNAFSQRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVFTRARALSVTKALKGM
                     FADLEEATDVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLAETAPKLRADR
                     ERINAARDKLRAETDILTSQRRGVVTPVSDIVSSLHEMSNSGRLDDIPEE"
     gene            8947..10653
                     /locus_tag="ACHLAG_003368"
     CDS             8947..10653
                     /locus_tag="ACHLAG_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP0656534.1"
                     /translation="MTFRKNEPRCDEPSEMTEAEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRMRPAQRQLFRSMHNKNIILKARQLGFSTAIDIYLLDQALFIPHLKCGIV
                     AQDKQAASEIFRTKIAVPFDHLPDWLRASFTIVERRSGASGGYILFGHGSSIQVATSF
                     RSGTVQRLHISEHGKICAKYPAKAKELRTGTLNAVSDECIIFDESTAEGVGGDFYEMS
                     NRAQEITASGLSLTAQDYKFHFYAWWQDPKYSARVPESGLKLSREKMTYFSAVEKAMN
                     ITLTDEQKQWYINKETEQREEMKQEFPSTPQEAFLTSGRRVFSAESTLQAESFCSPPL
                     IVYDIEPVTGRKTKAQSLRDGNKAEQHRTLMNYLLVWELPDPDEEYVCGADIAEGLEH
                     GDRSSLDIIRCSNGEQVAHWFGHLDAELFAHLIAQVCRMYNNAFVGPERNNHGHAVIL
                     KLRELYPTRYIYNEQHLDQAYDDDTPRLGWLTTRQSKPVLTEGMKTLLNNGISGIRWS
                     GTLSEMNTYVYDAKGSMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWM
                     AH"
     gene            10653..12797
                     /locus_tag="ACHLAG_003369"
     CDS             10653..12797
                     /locus_tag="ACHLAG_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFP0656535.1"
                     /translation="MKNETNTMATKNDNGATPRFSQRQLQALCSDIDSQPKWRDAANK
                     ACAYYDGDQLPPEVLQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLVVMSDEPDD
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKAGLSWVEVRRNSDPFGPEFRVST
                     VSRNEVFWDWLSRESDLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TITEGQPSPLMSAWEEYQSWDRRENEWLQRERRRVLLQVVYYRTFERLPVIELSNGRV
                     VAFDKNNLMQAVAVASGRVQVKVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDSDLMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QDSGATSGVAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQEVAAQQQALQQ
                     QQAELQMREMAGRVAKLEADAARAHAAAQRDNASAQREVALTQGQRYVDALNQAHTAE
                     IITGVQNMEQEQDVLQQQMLYTLQQRMNEMSL"
     gene            12955..13962
                     /locus_tag="ACHLAG_003370"
     CDS             12955..13962
                     /locus_tag="ACHLAG_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656536.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDSHAQDVVTEDTTEKHAD
                     EEAQTQTGDNNVAPTPDASVEQTQDVKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRRVELLTSQIHQAGMKPTPLPENEKISDEQIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSTQQAEGNGGEDLSPVLDAMNAVPVLKTWQESDPDRFSVA
                     VSIDGKLQNDPAWKDKTLTERFAEVARRTQVAFGEVSESSADNQADKTDIRKTAEEKV
                     KTAEREQAVPASPSDLGTTASVGTGDNFERLLGASHSEAEAIMRGMTNAEIDALLEKL
                     G"
     gene            13986..15200
                     /locus_tag="ACHLAG_003371"
     CDS             13986..15200
                     /locus_tag="ACHLAG_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFP0656537.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQEAPKAVLPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTAEHPEFKKIMINDVLPPTHDRHFFGGDATSFEQIEAADIFSIGLVDNLSL
                     FIDEMAHPLQPVRLSGDELHGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMVRAVNRA
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFYQGSKVLVSENNLTATTKEVAAATNIDR
                     AMLLGAQALANAYGQKAGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            15256..15645
                     /locus_tag="ACHLAG_003372"
     CDS             15256..15645
                     /locus_tag="ACHLAG_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656538.1"
                     /translation="MAKTILAPSLSERVYTGTHGNESVAEGVFTVNAAEADSVIHLLS
                     LPVGIRINSLQLVSTGGLGTATVSIKSGEHVLIDNSEAVSAKFARYVPVEPYTTQRDG
                     ELVTVTIKTAAATGTLNVLLRYTVVGY"
     gene            15695..16156
                     /locus_tag="ACHLAG_003373"
     CDS             15695..16156
                     /locus_tag="ACHLAG_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656539.1"
                     /translation="MTEKIAVVYIGPKPVKKDTITGSRTLFPRLEPVHVDSAMAWQLL
                     GFPDVWVRHEELDDVLKKQQQNEQLRQAQQAQERVLAARAEAENSFVVSVNGQEVDLS
                     KLTSARLATLCEAEELDIHKDPKETAEAFRIRVREAFRRRVAETEQHGGTE"
     gene            16140..16703
                     /locus_tag="ACHLAG_003374"
     CDS             16140..16703
                     /locus_tag="ACHLAG_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656540.1"
                     /translation="MAELSDFLPYVRRHISGPLNIMMTDALSMAAVAFSRQSLVCRRE
                     VTVVPVAGKEIVLPYDKDDEECVHIIRISDDNHELFVGRDVDISSGRSLRFACSPGEV
                     SVLYAVAPKAGRSQIPDELLTWPEEVAAGALERLFMQTGVSWSDPLRAQYFSVQFSEG
                     IRRAYRHTLATSPYSSYRNPVRRQRFY"
     gene            16703..17353
                     /locus_tag="ACHLAG_003375"
     CDS             16703..17353
                     /locus_tag="ACHLAG_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFP0656541.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLQELCDYYNDAVRAVILARP
                     DAGASLETISCVPGARQVLPDGVIQLLDVICLSDGSAVRPLSREVLDAQYPEWPTMKG
                     IPECFISNDLSPRVFWLFPVPDKEISIDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSALSARKKVFNGGGV"
     gene            17350..>18221
                     /locus_tag="ACHLAG_003376"
     CDS             17350..>18221
                     /locus_tag="ACHLAG_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFP0656542.1"
                     /translation="MSVVVSGTLKSPDGEAISGANITLTALTVSPDALSGTSASAVTR
                     EGGYYGMTMDPGEYAVSVTVKGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTATKNTQATQSKESAAASAKSASDSAKTATSRAA
                     EAGQKATDATEAATRAITAAGNAEESSTRAGESEKAAGADAEKARQHAEKARLAQESA
                     GEILKRAEAATVSAEEARRMAENARGPRGPQGETGPKGDVGPKGETGPVGPQGPAGPR
                     GEKGEQGERGPQGIP"
BASE COUNT         4695 a         4134 c         5157 g         4235 t
ORIGIN      
        1 gagtttgcca gcccccccag tggctggctt ttttatgtcc gtaacatcct gtgtatcaat
       61 aaatgttgtt gtctacgtac gtcaagtagt cgcatgagat ctgaccagat atgttaaggt
      121 tgcagctctc tttgaatata attatcattt tcattacgtt attgttacgt ttatccggtg
      181 cgccgtaaaa cgccgtcctt cagggggtgg aggatgtcaa gaatatagtt atcgtatggt
      241 gctcaaggag tattgtgtaa tatgaaaata attattttta gagtgctaac ttttttcttt
      301 gttatctttt cagttaatgt ggttgcgaag gaatttacct tagacttctc gactgcaaag
      361 acgtatgtag attcgctgaa tgtcattcgc tctgcaatag gtactccatt acagactatt
      421 tcatcaggag gtacgtcttt actgatgatt gatagtggca caggggataa tttgtttgca
      481 gttgatgtca gagggataga tccagaggaa gggcggttta ataatctacg gcttattgtt
      541 gaacgaaata atttatatgt gacaggattt gttaacagga caaataatgt tttttatcgc
      601 tttgctgatt tttcacatgt tacctttcca ggtacaacag cggttacatt gtctggtgac
      661 agtagctata ccacgttaca gcgtgttgca gggatcagtc gtacggggat gcagataaat
      721 cgccattcgt tgactacttc ttatctggat ttaatgtcgc atagtggaac ctcactgacg
      781 cagtctgtgg caagagcgat gttacggttt gttactgtga cagctgaagc tttacgtttt
      841 cggcaaatac agaggggatt tcgtacaaca ctggatgatc tcagtgggcg ttcttatgta
      901 atgactgctg aagatgttga tcttacattg aactggggaa ggttgagtag tgtcctgcct
      961 gactatcatg gacaagactc tgttcgtgta ggaagaattt cttttggaag cattaatgca
     1021 attctgggaa gcgtggcatt aatactgaat tgtcatcatc atgcatcgcg agttgccaga
     1081 atggcatctg atgagtttcc ttctatgtgt ccggcagatg gaagagtccg tgggattacg
     1141 cacaataaaa tattgtggga ttcatccact ctgggggcaa ttctgatgcg cagaactatt
     1201 agcagttgag ggggtaaaat gaaaaaaaca ttattaatag ctgcatcgct ttcatttttt
     1261 tcagcaagtg cgctggcgac gcctgattgt gtaactggaa aggtggagta tacaaaatat
     1321 aatgatgacg atacctttac agttaaagtg ggtgataaag aattatttac caacagatgg
     1381 aatcttcagt ctcttcttct cagtgcgcaa attacgggga tgactgtaac cattaaaact
     1441 aatgcctgtc ataatggagg gggattcagc gaagttattt ttcgttgact cagaatagct
     1501 cagtgaaaat agcaggcgga gattcataaa tgttaaatac atctcaattc agtcagttgt
     1561 tgccggtctg ataatagatg tgttagaaaa tttctgcatg gtgaatcccc ctgtgcggag
     1621 gggcgactgg tgaacggtat gatctctttg atgatcgtaa gcgagaatac gcgggtttgg
     1681 tggcaccaga ccgaactcac cgggaggcac ccggcaccat gcagtattca gagattaggc
     1741 atatatccag gctcctcatc gcaggagcct ttttacatgc aaaaaaaaac cgctcctggg
     1801 aagagcggct ggcaagaaga aacaatatga acaattaatt aacgatggga ataatacctt
     1861 agagtaatca ccttgcgcaa ctgtaagggc atatttcatc tttgcgggct gtttttctgt
     1921 gtggcttctg tgtttccgga gggcagcctg taccttttct gactcagaac attatcccgg
     1981 ccgggaggat tcatggcatt taaacactac gatgtggtca gggcggcatc gccgtcagac
     2041 cttgctgaac gaataactca aaaactgaag gaagggtggc agccttatgg tagtgcgctg
     2101 atttcgacag ctggttatgg tgcagagttt atccagccag ttgtgagtga gggggagctt
     2161 ccctcacttg cagagtcagg caatcacccg catgtttcag cgaagcctga agcagcgccg
     2221 gaatattact atgtgattgc gcttgccggt cagtccaata gcatgtcata cggtgaaggt
     2281 cttccgttgc cggagacata tgaccgtccg gacccgcgta ttaagcagct ggcgcgccgc
     2341 agtacggtga caccgggcgg tgcagcatgc aaatataacg acatcattcc ggcggaccat
     2401 tgtctgcatg atgtgcagga catgagccgt cttaaccatc cgaaagcgga cctgtcaaag
     2461 gggcagtacg gaaccgtggg gcaggggctg catatcgcca aaaaactgct gccgtttata
     2521 ccggcgaatg cgggtattct gctggttccg tgctgtcgtg gtggttcagc gttcaccacc
     2581 ggagctgatg gcacatacag tgacgctggc ggtgcctcgg agaattcaac ccgctggggt
     2641 gtggacaagc cgctgtataa ggaccttatc ggtcgaacaa aagcagcact gaagaagaac
     2701 ccgaaaaatg tgctgtttac cgtggtgtgg atgcaggggg aatttgattt tgacggcacg
     2761 cccggaaatc acgcagcaca gtttggtgcg ctggttgata aattccgtgc agacctgacg
     2821 gatatggcag gtcagtgcgt tggtggctct gctggcggtg ttccctggat atgcggggac
     2881 acgacgtatt tctggaagca gaagaacgaa tccacgtacc agacggtgta cggcagctac
     2941 aaaaacaaaa cggaaaagaa tattcatttc gtaccgttca tgacggatga gaacggggtg
     3001 aatgtgccga cgaacaaacc ggaagaagac ccggacattc cgggtatcgg atattacggt
     3061 tcgaaatggc gtgacagctc agccacctgg acgtcacagg acagggcgag ccatttcagt
     3121 tcatgggcac gccgcgggat tatttccgac cgtctggcaa cggcgattct gcgccatgcg
     3181 ggaagagtgg cgctaaacgc gggggcatca tcgacagtat cagaggtgcg cccgtcatcg
     3241 ccttccggtg cagaagccac aggcgtcaca acactgctct cttaccttgc cagcgagtca
     3301 gagggaagcc tgaaagtaca gggatggtca gccagtggcg gcagggcaga agtggtcagc
     3361 gatgcggagg gaaccggagg taaggctgtg aaggtgacca aggaagccgg taaaagcacc
     3421 tgggtgctgg agtacgctgc gggcaacggc gcggctctgt tacagaaagg gggacagatt
     3481 cgctgccgct ttaaggcttc gggagcgctg gcggcgaacc agtatgttat ggcgttttac
     3541 tggccggtat cgtcactgcc acagggcgtt gtcctgaccg gcgatggggg gaataacctg
     3601 ctggcagcgt tctacatcca gacggatgca aaagacctga atgtgatgta ccacaatgcg
     3661 aaagtggcga caaacaacct gaaactggga agctttggcg catttgataa cgaatggcat
     3721 acgctggctt tccgctttgc cgggaataac agccttcagg tgacgccggt tattgatggt
     3781 caggatggca caccgttcac gctgacgcag tcaccggtca gtgcattttc ggcggataag
     3841 ttgcatgtga cagacattac caaaagtgcg acttacccgg tactgataga cagcattgcg
     3901 gtggaagtga ataacgcgga cgccgctgca tgataaaaaa agccgccagt taccggaatg
     3961 gactggcggt ggtaatacct atggagaaaa aataaaggaa cgatactttc gtactctgtt
     4021 tttttaaatg aaaacagttc ttattgtcaa caataacgga aagaaattat gacatttctg
     4081 aaccagttaa tgctgtactt ctgtacggtg gtctgtgtgc tgtatctcct ttcgggtgga
     4141 taccgggcca tgcgtgactt ttggcgcaga cagattgaca aaagggccgc tgagaaaatc
     4201 agcgccagtc agtcagccgg aagcaaaccc gaagagccgc tcgtttagcg gcaactttct
     4261 taatcacacc tttcgacgag aaaatcccat gtcagaaatt acatccctgg tcactgctga
     4321 agcagtgaag gacgtcctgc gctctgaaga agtcctgagt gcactgaaac agaaacttcg
     4381 ccataacctg gaagcgcgtc ttgatgcaga agtggatgcc attctggatg aactgctggg
     4441 cgcaccggca gctccggagc cggaaggcat cgcgggagag gggagtgctt cagatagcgg
     4501 tgaccccaca cctgacagcg acatgatgat gtaagcatgc gtcagggacc atcggtgtgt
     4561 gccggtggtc ttttttattg ttgtgagctt ccggattgcg ggaggcgggg tatgtaccag
     4621 atggaaaaaa tcacaacagg tttgtcatac accacgtcag cggtgggaac gggctactgg
     4681 ttcctgcagt tgctggacag ggtttccccg tctcagtggg cggcaatagg cgtgctgggg
     4741 agtctgctgt ttggtctgct gacgtacctg acgaacctgt atttcaaaat cagagaggac
     4801 cggcgtaagg cggcgcgggg agagtaagct gatgagccgg aaattccgct atggtttatc
     4861 agctgtcgtt ctggcgctga ttgccgcagg ggcttctgcg cctgaaatcc tggatcagtt
     4921 tctggatgaa aaagaaggta accacaccac ggcataccgt gatagtgcgg gtatctggac
     4981 catctgccgt ggagccaccc gggtggatgg taagcctgtt attcctggca tgaagctgtc
     5041 gaaggaaaaa tgcgaccggg ttaacgccat tgagcgtgat aaggcgctgg catgggtgga
     5101 gaaaaacatc aaagtgccac tgactgaacc ccagaaagcg gggatcgcgt cattctgccc
     5161 gtataacatt ggccccggta agtgtttccc gtcgacgttt tataaacgaa ttaatgcagg
     5221 cgatcgccgg ggtgcatgcg aggcgattcg ctggtggatt aaagacggtg gcagagactg
     5281 ccgtatccgc tcaaataact gttatggtca ggtatcccgt cgcgaccagg agagtgcgct
     5341 ggcgtgctgg ggaatcgaca gataagcaga atattttgct gaaaaatgac gttggccaac
     5401 gcgggcggat aacacgaaat cctgcgaact ggcaaaacct aagtgaataa agtcaggaac
     5461 attgtttcac gctgaggcac cgtaatggtg tctttgtcat ttctgcgctt cgcacaagcg
     5521 taaataaacc aaagaacctt tcaggatgag ccctggtgga taaccggctg gtctggttaa
     5581 ccctctttgg gctggttatt cctgtgcgca gggttcatca ctaaaaggaa ataaccgatg
     5641 aatatgatga ccgtgccgtt tcacggcgat tctctttatg tggttaacca taatggcgaa
     5701 ccgtatgttc caatgaaacc cattgttgag gggatggggc tggcttgggc tccacaattt
     5761 actaagttaa aacaacgatt tgccaaaggc gttacggaaa tcataatacc ttctgctggt
     5821 ggtgagcaaa caatgacttg tcttgctctg cgtaagctgg ctgcctggct tcagaccatc
     5881 aactcaaaca aagttaaacc tgaaattcgc gacaaggtaa tccagtatca ggaagagtgc
     5941 gacgatgttc tctatgaata ctggacgaag ggggttgtcg ttaatccccg tcgaatgagt
     6001 gtgatggaag aactcaatca ggcttgcgct gacatgaaac gggataaaaa cattgccagt
     6061 gtgtttgcta ccgggctgaa tgagtggaaa caggttaaag ccgcgcatgt atcaaaaatc
     6121 cgcacattga taaacgaagc gaatctgctg attgattttg tcctagctga tacaggcaaa
     6181 gggaaaataa caaaggcgga ttgatggagt agtggctaat gatatcggat aaactcataa
     6241 cgctggcgaa gatcctctgt gtaatcgtcg gcatttcatt tttagtcatg ctggttgcca
     6301 ttttcttttc caccgcctgg cgagtcctga cgttatcggg actggtgggg tgaaagagag
     6361 atgaaccgtg ttctgtgtgt ggtgattatt gtcctggcgg ttggctgtgg tgcgctgtgg
     6421 ctggcaacaa accattaccg tgacaacgcg ctcacctaca aagcgcagcg agataaaaaa
     6481 gtcagagagc tggagctggc aaacgcaacc attactgata tgcaggtgcg ccagcgtgat
     6541 gttgctgcgc ttgatgctaa atactcgagg gaattagccg atgcgagagc tgaaaatgaa
     6601 actctgcgtg ctgatgttgc cgctggtcgt aagcgcctgc ggctcaacgc cacctgcccc
     6661 ggtaccgtgc gtgaagccac cggcacctcc ggcgtgggca atgatgccag cgtcgaactc
     6721 tctccagttg ctggacgaaa cgttctcggt atcagagacg gaatcatcag cgaccaggca
     6781 gcactgagaa cgcttcagga atatatccgc actcagtgct tgaattaaca gagccagcat
     6841 gatcgctggc tttttcatat ctgaatttca ccgcgcaccg cagcgcatga taaccaccga
     6901 acctgcccct ttggaatgag cctttgagga taccagttag tgctggcgag cctcggtggg
     6961 ctggtttcct atgcggcaaa ggttcatttc aaagaacagg tatacgacat gaaatcatta
     7021 accctcttca atcagccaat ccgtgtcgct gaagatggca tgatctgcct cactgatatg
     7081 tggaaagcca gtggtaaaag tgaatctgaa tctccatacc actacctgag aaacaagcag
     7141 accaaagagt tcttagccga gctggagaaa aaccacgaat ctgtggtttt tactgagcgc
     7201 ggtgtacacg gtggaacata tggcgggaag tttgttgctt atgattatgc agcatggcta
     7261 aaccccggat ttaaatatgc agcctataaa gtcctggatg actacttcac cggagaactt
     7321 cagcatcgca acagcttaag tgcgcagctc aatatgaagt gccatgagtt tgatcagaaa
     7381 aaagatatgg cgagcttctg tggacaaggg ctcgcggcat ggcgctatac gaagccagtg
     7441 ttggtcgctg agattaactc cttggctaac cagctgcaga ttacgatccc cgggcttccg
     7501 ggatgagtga tcgtgtcatt gaatgcgcct ccagagcggg gcgcgacttc tcagagttca
     7561 tgaaaggcga gaagggcatg atggaagcat tggcctcggt ggatgagttt ggcgagcagc
     7621 tgcgcctcaa cggctgtgtc aatcatcact ttgtcagcta catgatgcgg aactcgatca
     7681 tgcaggcatt catggacatg gcaaaagccg agaggaaaga agagcgccgg cgtaagcgag
     7741 cggaagcaaa agcgaagtag ttattacaaa agccattccc tacagagtgg ctttgataat
     7801 ggcttatacc ctacacggga taacttaact gatatccctt ttaacggata aaggcatttc
     7861 agcctgacat agccatgcgc cgcatcgtcg ccgtattccc gcattaacag agaccgcagc
     7921 caccttatct gcgtgagtgt gcggggataa tcaaaaacga tgcacaccgg gttttctcat
     7981 ttttcacgag atgggagcga tttcccgcga agccgcctgt ccggtgcggt ggtggaagaa
     8041 accggataaa acaaccgcat tgtgcaaata tcgatcaaat atggtgctgc tgtgtgaaat
     8101 ctgaaaaatc acagcggtca ttatgcatca gtttttaaca caggacgtca gaacgtgaca
     8161 tggcaaagct ggactggaaa aagctggagc aggcattccg acgcgaacat gccgaaacgg
     8221 gaataacatt actggactgg tgccggaaga aaaagattaa ttacaacacc gccagaaccc
     8281 gtataaaaat gggcaaaatc gatcatgaaa ttgatcataa aaccgatcat gaaatcgatc
     8341 atgacatctc agatgaagaa ccctgcaatg acgcgggttc cggcgatgaa aaatgcgcaa
     8401 aaaactctga aaaaaactgc gcaaattcgg cagaaacgaa acggattcgt ggttcccgac
     8461 ttttaccacc ttcaaacgct ttttctcagc gaaacaccca cgctgtaaga caccgtggat
     8521 acgcgaagta tcttgaggca gataacctca tggatgatgc gtccgatatg gtgctgttcg
     8581 atgaactggt gttcacccgg gcccgcgcac tttcagtaac taaggcactt aaagggatgt
     8641 tcgccgacct ggaagaggca actgacgtgg aaacccgtgt tgctctttac gacaaaatac
     8701 tcaaagctga acaggccctt gaccggaata ttgcccgtat cgagtcaatt gaacgctcat
     8761 tgctgacgct ggacgtcctg gctgagacag caccaaaact tcgtgctgac cgggaaagaa
     8821 tcaacgccgc cagagataaa ctcagagctg aaaccgatat tctgaccagc cagcgtcggg
     8881 gcgttgttac gcctgtcagt gacatcgtgt catcgctgca tgaaatgagt aattcgggga
     8941 gactggatga cattccggaa gaatgaaccg cgatgtgatg agccgtcaga aatgaccgag
     9001 gctgaacaac gtctgttcat catgactaaa ctgagcaatc cctggtggcg gctcaatcat
     9061 ctctacaaaa tacagaacga aaaaggtgaa ctggtcacct tcagaatgcg accggcgcag
     9121 cgccagttgt tccggagcat gcacaataaa aatattatcc tgaaagcgcg ccagctggga
     9181 ttttccacag ccattgatat ttatcttctc gaccaggcat tattcattcc gcatctcaaa
     9241 tgcgggatcg tcgctcagga taaacaggct gccagtgaaa ttttccgcac aaaaattgct
     9301 gtaccgtttg atcatctccc tgactggctg agagcctcat tcaccatcgt tgaacgtcgt
     9361 agcggtgcca gcggtggcta tatcctgttt ggtcacggct cgagtatcca ggtggcaacc
     9421 tcattccgtt caggtacggt gcagcgcctg catatctcag agcacggcaa aatttgcgcg
     9481 aaatatccgg ctaaggcgaa agaactgcga accggtacgc ttaatgccgt ctctgatgaa
     9541 tgcattattt ttgatgagtc cacagctgaa ggcgtgggtg gtgattttta cgagatgagt
     9601 aaccgagcac aggagatcac agcatcaggc ttatcgttga cggcacagga ttataaattc
     9661 catttttacg cctggtggca ggatcctaaa tacagcgccc gagtgccgga aagcgggctg
     9721 aagctgtcac gggaaaaaat gacgtatttt tctgcggttg agaaggcaat gaacatcacg
     9781 cttactgatg aacagaagca gtggtacatc aataaggaaa ctgaacagcg tgaggaaatg
     9841 aagcaggagt ttccctcaac gccacaggag gcgtttctga cgtccggacg acgtgtgttc
     9901 agtgccgaaa gtacgttgca ggcagaatca ttctgttcgc caccgctgat tgtttatgac
     9961 attgaacctg ttacaggaag gaagactaaa gcgcagtctc tgcgtgacgg gaataaagcc
    10021 gaacagcacc ggacgctgat gaattatctg ctggtctggg agctaccgga tccagatgaa
    10081 gagtatgtct gcggagcgga tattgctgag gggcttgagc acggggaccg ctcatcgctg
    10141 gatatcatca gatgcagtaa tggtgagcag gtggcccact ggtttggtca tcttgatgcg
    10201 gagctttttg ctcatctcat tgcgcaggtc tgtcgtatgt acaacaacgc gtttgtgggg
    10261 ccggagcgta acaatcacgg acatgccgtt attctgaaac tcagggagct ctatccgacg
    10321 cgttatatct acaacgaaca gcaccttgat caggcatatg atgacgatac gccccgcctt
    10381 ggctggctga caacccgtca gagcaaacct gttctgaccg aaggaatgaa aacgctcctg
    10441 aataatggaa tatcagggat ccgctggtca ggcacattat cggaaatgaa cacctacgtt
    10501 tatgacgcga aaggctccat gaatgcacag gaaggctgtt ttgatgatca actcatgagc
    10561 tacatgattg cccaggagat gcgcgccaga atgcctgtga gggtaaaaca gaaaacggat
    10621 aaacgcagaa ctacacactg gatggctcac tgatgaaaaa tgaaactaac accatggcga
    10681 cgaaaaacga caatggagcc acgccgcgtt tttctcagcg ccagttacag gcgctttgtt
    10741 ctgatattga cagccagcct aaatggcgtg atgccgcaaa caaggcctgt gcgtattacg
    10801 atggcgatca gttgccaccg gaagttcttc aggttctgaa agatcgcggt cagccgatga
    10861 ctatccataa cctcatcgcg cctaccgtcg atggcgttct gggaatggag gccaaaacac
    10921 ggactgatct ggtggtgatg tcagacgagc cagatgatga aactgaaaaa ctggctgaag
    10981 ctattaatgc tgaatttgcc gatgcatgcc gccttggcaa tatgaataaa gcccgctctg
    11041 atgcctatgc ggaacaaatc aaggcgggcc tcagttgggt ggaggtcagg cgcaacagcg
    11101 atccgttcgg acctgaattc agggtatcca cagtcagcag aaatgaggtt ttctgggact
    11161 ggctcagccg ggaatccgac ctgagtgact gccggtggct gatgcgtcgc cgctggatgg
    11221 ataccgatga ggcaaaagcc acgttcccgg gaatggctca ggttatcgat tatgctattg
    11281 atgactggcg tggttttgtc gataccacga ttactgaagg ccagcccagt ccgttgatga
    11341 gtgcatggga agagtatcag tcatgggatc gcagggagaa tgaatggctt cagcgtgaac
    11401 gccgtcgtgt gctgcttcag gtggtttatt accgtacatt cgagcgtctt ccggtgattg
    11461 aactcagtaa tggacgggtg gtggcttttg ataaaaataa tctgatgcag gcggtggctg
    11521 tggcatccgg gcgggttcag gtgaaagtcg ggcgggtaag tcgtattcgt gaagcctggt
    11581 ttgtgggccc acactttatt gtggatcgcc cctgtagtgc accgcagggg atgtttccgc
    11641 tggttccttt ctggggatac cgaaaggata aaaccgggga gccatacggg ttaatttccc
    11701 gcgccattcc ggcacaggat gaggtgaatt ttcgtcgtat caagctgacc tggctgcttc
    11761 aggccaaacg cgtgattatg gacgaggatg ccacccagtt gtcagacagc gacctgatgg
    11821 agcagatcga acgtccggat ggcattatta aactgaatcc ggcccgaaaa aatcagaaaa
    11881 gtgtcgcaga tgtttttcgg gttgagcagg attttcaggt tgccagccag cagtttcagg
    11941 tcatgcagga atcggaaaaa cttatccagg ataccatggg agtgtattct gcatttctcg
    12001 ggcaggattc aggtgcgacg tcaggcgtgg ctatcagtaa cctggtggag cagggggcca
    12061 caacccttgc ggaaatcaac gataactacc agtttgcctg ccagcaggtg ggaagactgt
    12121 tgctggctta tcttctcgat gacctgaaaa aacgccgtaa tcatgcagtg gtgattaatc
    12181 gcgatgatcg ccagcgtcgc cagaccattg tcctcaatgc tgaaggtgat aatggtgaac
    12241 tgaccaatga tatttcaagg ttaaatacac atattgcgct ggcgcctgtt cagcagacac
    12301 cggcgtttaa ggcacagctt gcacagagaa tgtcagaggt tattcagggg ctgccgcctc
    12361 aggtgcaggc tgttgtgctc gacctgtggg ttaatcttct ggatgtgccg cagaaacagg
    12421 agtttgttga gcgtattcgt gctgcgctgg ggacgccaaa atcaccggat gaaatgacgc
    12481 cggaagaaca ggaagtagcg gcacaacaac aggcacttca gcaacaacag gcagaactcc
    12541 agatgcgcga gatggctggc agagtggcaa aactggaagc tgacgccgcc agggcacatg
    12601 cagctgcaca acgggataat gccagtgcac agcgggaagt cgccctgaca caggggcagc
    12661 gttatgtgga tgcgcttaac caggcacata cggcagaaat cattaccggc gtacagaata
    12721 tggaacagga gcaggacgtt cttcagcaac agatgctgta tacgttacaa cagcggatga
    12781 atgaaatgtc gctctgaaaa ctctggcttc aactgaaccc cgtcatcgta cggggttttt
    12841 tgtttccgga ggtaagcgtt ccgggagcgg tgcgcttatt cgcgggggca gcgataagcc
    12901 ttatttactc aaccattcgg atctgtccga taaacagacc atgcggagtt atttatggat
    12961 tttgaattta cgggtgaaga aaccccggaa caactggaaa aaatgctgga aggacttggg
    13021 gatgtggata ttgacagtca cgcacaggac gtcgtgacgg aagataccac ggaaaaacat
    13081 gcggatgagg aagcacagac tcagacgggc gataacaatg tggcaccgac gccggatgcc
    13141 agtgtggagc agacgcagga cgtgaaggag ccggaagcga agggggtgct cacccgcgac
    13201 ggtaaacacg tcattcccta tgaagtcctt gaggctgaac gttccggtaa gcaacgggcc
    13261 gaacaggaag ccgcacttct tcgtgggcag atagctgaag aaaaacgcag ggtggaactg
    13321 ctgacgtctc agatccacca ggccggtatg aagcccacac cgttaccgga aaacgaaaaa
    13381 atttctgatg agcagattgc ccgtatcagg gagatgtatc cggaaattgg tgacgcggtg
    13441 gcttcgctca tccgtaaaaa taactatctc cagtcccgtg ttcagcaatc aacacagcag
    13501 gcagaaggta atggtggtga ggatttatca ccggttcttg atgcgatgaa tgccgtgccg
    13561 gtgctgaaaa cgtggcagga gtccgatccg gatcgcttct cggttgctgt atccatcgac
    13621 gggaagctcc agaatgaccc cgcatggaaa gacaaaacgc tcactgaacg tttcgctgaa
    13681 gtggcccgtc gtacgcaggt tgctttcggt gaagtcagtg agtcgtctgc tgacaaccag
    13741 gcagacaaaa cggatatccg gaaaacggcg gaagagaaag tgaagaccgc tgaacgggag
    13801 caggcagtac cagcttcccc gtcagattta ggcaccacgg cctccgtcgg aaccggtgat
    13861 aattttgaac ggttacttgg cgcttcgcat tcagaggcag aggcgattat gcgcggtatg
    13921 acgaatgctg aaatagacgc acttctggag aagctcgggt aacttactga aggagtactg
    13981 aagtaatgac gactgtaaca tcagcccagg cgaataagct gtatcaggtg gcgcttttta
    14041 ctgctgccaa ccgcaaccgc tcgatggtta atatccttac tgaacagcag gaagcgccaa
    14101 aagcggtttt gccggacaag aaaagcacga agcagaccag cgcaggtgcg ccggttgtcc
    14161 gtatcacaga ccttaacaaa caggccggtg atgaagtgac cttcagcatc atgcacaaac
    14221 tctcaaaacg tccgacgatg ggagatgagc gtgttgaagg tcgtggtgag gatctcagcc
    14281 atgctgactt ctccctgaaa atcaatcagg gacgtcacct ggtggatgca ggcggacgta
    14341 tgagtcagca gcgcacgaag tttaacctgg catcctctgc cagaacgctt ctggggacgt
    14401 actttaatga cctgcaggac cagtgtgcga tagtgcatct tgcgggagct cgtggtgatt
    14461 ttgttgctga cgacaccatt ctgccgacag cggagcaccc tgaattcaaa aaaatcatga
    14521 tcaacgatgt actgcctccg acacatgacc gtcacttttt tggcggtgat gcgacaagct
    14581 ttgagcagat tgaagcggca gatatttttt ctattggcct ggtggacaat ctctccctgt
    14641 tcattgacga aatggcgcat ccgttacagc cggttcgtct gtccggtgat gaacttcacg
    14701 gagaagatcc atattacgtc ctgtacgtca cgccgcgtca gtggaatgac tggtacacct
    14761 cgacgtccgg taaggactgg aaccagatga tggttcgtgc cgtgaaccgt gcaaaaggtt
    14821 ttaatcatcc gctgttcaaa ggtgaatgtg cgatgtggcg caatatcctg gttcgtaagt
    14881 atgcgggtat gccgatccgt ttctatcagg ggtcaaaggt tctggtatca gagaataacc
    14941 tgacggcaac cacgaaagag gtcgctgctg caaccaatat tgaccgcgcc atgttactgg
    15001 gggctcaggc gctggcaaat gcttacggtc agaaggcggg cggtcacttc aacatggttg
    15061 agaagaaaac ggatatggat aaccgtactg agatagcaat aagctggatc aacggtctga
    15121 aaaaaatccg tttccccgag aagagcggca agatgcagga tcacggcgtg attgccgttg
    15181 atacagcagt gaagctctga ttttttcctt tccccatgcc gggttttcgc ccggcttttt
    15241 caggagtcat taattatggc aaagactatc cttgccccgt cactgagtga acgggtctat
    15301 acgggtacgc acggtaatga gtcggtggca gaaggcgtat ttacggtgaa tgctgcggaa
    15361 gcggacagtg ttattcatct tctctcactg ccagtgggca tccgtatcaa ctcactccag
    15421 ctggtttcaa cgggtggtct gggtactgca accgtcagca ttaagtccgg tgagcatgtt
    15481 ctcatcgata acagcgaagc tgtttctgca aaatttgcca gatatgtgcc agtggagccg
    15541 tacaccacac agcgtgacgg ggagctggtt actgtcacca ttaagactgc cgctgcaacc
    15601 ggcaccctga atgttctgct gcgttatacc gtggtgggat actgattaaa accttccggc
    15661 ccgcgtcatg cgggcttttt atccggggaa ttatatgact gagaaaattg ccgttgtcta
    15721 tatcggccca aaacccgtga aaaaggacac cattaccgga agtcgcacgc tgttcccacg
    15781 tcttgagccg gtgcatgttg acagtgcgat ggcctggcaa ctgctggggt ttccggatgt
    15841 ctgggttcgt catgaagagc ttgatgatgt tctgaaaaag caacaacaga atgagcagtt
    15901 gcggcaggca cagcaggcgc aggaaagagt gcttgctgcg cgggcagaag cggagaacag
    15961 ttttgttgtt tctgttaacg ggcaggaggt ggatttaagt aagctcacct cagcacggct
    16021 ggcgacgctg tgtgaggcag aagagctgga tattcacaaa gacccgaaag aaacggctga
    16081 ggcattccgt atccgggtgc gtgaggcatt tcgccgtcgt gttgcggaga ctgaacagca
    16141 tggcggaact gagtgatttt ttaccgtatg tccgtcgtca tatcagcggt ccactgaaca
    16201 ttatgatgac ggatgctctg tcaatggctg ctgtggcatt cagccgccag tcgttggtgt
    16261 gccgtcggga ggttactgtt gtaccagtag caggaaaaga aatcgtgctt ccgtatgaca
    16321 aagatgatga ggagtgcgtt catatcatcc gtatctctga cgataatcat gagctttttg
    16381 tcggtcggga tgtggatatc agctccggac gctccctgcg atttgcctgt tctcccggtg
    16441 aggtgagcgt gctttatgcc gtcgctccga aagccggacg cagccagata ccggatgaac
    16501 tcctcacatg gcctgaagaa gtggctgcgg gggcacttga gcggttgttc atgcagactg
    16561 gtgtttcatg gtcagatccg ttacgcgctc agtatttttc tgtgcagttt tctgagggga
    16621 tccgtcgggc atatcgtcat acactggcga caagcccgta ctcttcatac cgcaaccctg
    16681 tacgcaggca gaggttttac tgatgacgac gattacagaa atcatcggac gtgtgaatac
    16741 acaactggtt gacccgatga tggttcgctg gcccctgcag gaattgtgcg attattacaa
    16801 tgatgctgtg agggcagtga ttctggcgag accggatgct ggcgcaagcc tggaaacaat
    16861 cagttgtgtt cctggcgccc gtcaggtttt gcccgatggt gtaatacaac ttcttgacgt
    16921 gatatgcctc agtgacggta gtgcagtcag accattatcc cgggaggtgc tggatgcgca
    16981 gtatcctgag tggcccacaa tgaagggcat tcctgaatgt tttatcagca acgacctgtc
    17041 cccgcgcgta ttctggctgt ttcctgtccc tgacaaagag ataagtattg atgcagtggt
    17101 aagccggata ccggaggcag tgtatgttct gacgcaggac gatgatacgc cagttccact
    17161 ggaagaggct tatgttaacc cactggtgga ctggatgttg tttcgtgctt tcagtaagga
    17221 tgctgccggt ggtgcagaat cggggctggc tgcgcagcat tatcagagtt ttgttgagca
    17281 acttgggatc aaacaggggg cagacagtgc attgtctgcc cgtaaaaaag tgtttaacgg
    17341 aggtggagtg tgagtgttgt tgtttcgggg acgctgaaat ctcctgatgg tgaggcgata
    17401 tcaggagcaa atattaccct gacggcgctg acagtttcac cggatgcgct cagcggcacc
    17461 agtgcgtcgg cagtgacccg tgaaggtgga tattacggaa tgacgatgga tccgggggag
    17521 tatgcggttt cggtgacggt gaaagggaag actgctgtct acggacgtgt gcgtattgag
    17581 gggaccgaaa gtacggtgac gctcaatatg ctgttacgcc gcagtcttgt tgaggtgagc
    17641 atacccggag agctgctgac agatttccgg cagatacaga ataatgtggc tgatgacctt
    17701 gctactattc gtcgcctgaa tgaagacacg gcgacaaaaa acactcaggc cacacagtca
    17761 aaagaaagtg cagcagccag tgcgaagagt gcatctgaca gtgcaaagac ggcaaccagc
    17821 agggcggctg aagccggaca aaaagcgact gatgccactg aggccgcgac ccgtgcaatc
    17881 acagcagcgg ggaatgcaga ggaaagctcg acccgtgccg gagagtctga aaaagccgcc
    17941 ggagctgatg cagaaaaagc cagacagcat gctgaaaagg ccaggctggc gcaggagagc
    18001 gccggagaga tccttaagcg ggcagaggct gccactgtca gtgctgaaga ggccagacgt
    18061 atggctgaga atgcacgggg gccccggggg cctcagggag aaactggtcc gaagggggat
    18121 gtcggtccta aaggcgaaac aggtccagtg ggccctcaag ggcccgctgg tccgcgtggt
    18181 gagaagggcg aacaggggga gcgaggaccg cagggaatac c
//
LOCUS       DAZGVQ010000102        18084 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000102 DAZGVQ010000000
VERSION     DAZGVQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18084
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.103"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(226..1818)
                     /gene="lsrK"
                     /locus_tag="ACHLAG_003377"
     CDS             complement(226..1818)
                     /gene="lsrK"
                     /locus_tag="ACHLAG_003377"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFP0656543.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(1897..2850)
                     /gene="lsrR"
                     /locus_tag="ACHLAG_003378"
     CDS             complement(1897..2850)
                     /gene="lsrR"
                     /locus_tag="ACHLAG_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFP0656544.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            3099..4634
                     /gene="lsrA"
                     /locus_tag="ACHLAG_003379"
     CDS             3099..4634
                     /gene="lsrA"
                     /locus_tag="ACHLAG_003379"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFP0656545.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4628..5656
                     /gene="lsrC"
                     /locus_tag="ACHLAG_003380"
     CDS             4628..5656
                     /gene="lsrC"
                     /locus_tag="ACHLAG_003380"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFP0656546.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKNREAA"
     gene            5656..6648
                     /gene="lsrD"
                     /locus_tag="ACHLAG_003381"
     CDS             5656..6648
                     /gene="lsrD"
                     /locus_tag="ACHLAG_003381"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFP0656547.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6660..7682
                     /gene="lsrB"
                     /locus_tag="ACHLAG_003382"
     CDS             6660..7682
                     /gene="lsrB"
                     /locus_tag="ACHLAG_003382"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFP0656548.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVTPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7709..8584
                     /gene="lsrF"
                     /locus_tag="ACHLAG_003383"
     CDS             7709..8584
                     /gene="lsrF"
                     /locus_tag="ACHLAG_003383"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFP0656549.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFILKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8608..8898
                     /gene="lsrG"
                     /locus_tag="ACHLAG_003384"
     CDS             8608..8898
                     /gene="lsrG"
                     /locus_tag="ACHLAG_003384"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFP0656550.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8955..9713
                     /gene="tam"
                     /locus_tag="ACHLAG_003385"
     CDS             8955..9713
                     /gene="tam"
                     /locus_tag="ACHLAG_003385"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFP0656551.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(9717..10631)
                     /locus_tag="ACHLAG_003386"
     CDS             complement(9717..10631)
                     /locus_tag="ACHLAG_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFP0656552.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10838..12289)
                     /gene="uxaB"
                     /locus_tag="ACHLAG_003387"
     CDS             complement(10838..12289)
                     /gene="uxaB"
                     /locus_tag="ACHLAG_003387"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFP0656553.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12516..13934)
                     /locus_tag="ACHLAG_003388"
     CDS             complement(12516..13934)
                     /locus_tag="ACHLAG_003388"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP0656554.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(14073..14432)
                     /locus_tag="ACHLAG_003389"
     CDS             complement(14073..14432)
                     /locus_tag="ACHLAG_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFP0656555.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14432..15358)
                     /gene="glsB"
                     /locus_tag="ACHLAG_003390"
     CDS             complement(14432..15358)
                     /gene="glsB"
                     /locus_tag="ACHLAG_003390"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFP0656556.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15422..16810)
                     /gene="sad"
                     /locus_tag="ACHLAG_003391"
     CDS             complement(15422..16810)
                     /gene="sad"
                     /locus_tag="ACHLAG_003391"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFP0656557.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16911..17792
                     /locus_tag="ACHLAG_003392"
     CDS             16911..17792
                     /locus_tag="ACHLAG_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0656558.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            17870..>18084
                     /gene="yneK"
                     /locus_tag="ACHLAG_003393"
     CDS             17870..>18084
                     /gene="yneK"
                     /locus_tag="ACHLAG_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFP0656559.1"
                     /translation="MVTPVSISNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRT"
BASE COUNT         4541 a         4618 c         4503 g         4422 t
ORIGIN      
        1 accccttttg catccattag tgtctttagg aatattcgct ataaaataag ggttgtgctt
       61 aatgctttaa gaaaaatagc aattttccct tgaatatagt actggtgatg gaacgatgaa
      121 tctgcaagct ggcttttaac aagccagctc taaaagaagg gaaataagaa taactatact
      181 caaaaactaa cagccacggt catcatgatg tggctgtcaa tgaaactata acccaggcgc
      241 tttccataac gacgtcgtca gtccatgatc aaccagcccc agctgatcct gataaactgc
      301 ctgccactta tcgcgtgaat cctgataaag ttcatgcttt tccgggtctg gtgtgtgcgt
      361 ccgttcccag cgaaccaggc gttctccggt ttctgccatt gatgaaaaaa ttccggcacc
      421 gacgccagct gcaatggcac atcctaatgc agtggcttct ttgaccaccg gaatattgac
      481 gggtaatccc gagacatcag cgagaatttg actccataat ttcccttttg aacctccgcc
      541 tgcaaagact aacgatgaag gatgaatatt cgagaaatca gcaatttgct gcaagttaca
      601 cgctgataca atcgccgcat tttcttccag cgcacggaac aatgtcgctt tgttacattt
      661 atccgggtca atggacaagt taataaagga aggcgcagcg tgataccagg ttttaaagcg
      721 cattctgtcg gagaagatcg gcattacgcc ccacgaccca ggcggcaccc gactggccat
      781 ctcttccagc agcgtatagg tgtcgatgcc taaacgttcg gcaatcagtt tttcttcggc
      841 acagaaagca tcgcggaacc agcgcatggt gagtccggta aaaaagctta tagattcagc
      901 ttgtaccatg ccaggaataa catgaggatt aacgcgcacg ttcatttctg ggtctgtcac
      961 cggcgcggct aaatttacaa cttgctgcca gaatgtgccg ccaagaaccg cggtttgtgc
     1021 cggacgcaca acgcctaacc caaggcaacc aagctgcacg tcgcctcctc caacgaccac
     1081 cggagtgccc gccttcagac cgcagagttc cgccgcttgt gaacttacca cgcccagcaa
     1141 tgtgccggtt tctttgacag gagaaagaat atcggcacgt aggccagcca tatccagcaa
     1201 tgcaggtttc cagtcacggg tggttagatc aagaagtccc gtggtgccag cgttagaggg
     1261 atccaccgcc agttcgccgc tgagcatata ggccagccag tcgctgatca tggtgatggt
     1321 tgatgcctga cggtaaatat cggaacgatg gtgcgccagc caaagtaatc tggggatggc
     1381 acttaaagcc agtgtttgtc cggtcgcgcg ataaacttcg ttttcaaagg tattgttgtg
     1441 cagttcttta agttcgctaa cttcgcgtgc cgctctggca tccacattgg cgcaggccca
     1501 gatcggggct ccttcattat tatataaaac aatgccttca cgcatcgaac atgccgaaac
     1561 ggcagcgata tactccgggg ctatgccggc gttgtgcagc gcctggcgca tacactcaca
     1621 cgccagttgc cagtttttgt tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc
     1681 caggtgccgc cactccgcct gtcccactgc tatttgattg ccttccaggt cgaatatcac
     1741 agcccgaata cttccggtac ctgcatccag cgccatcagg taatactttg attctgaagg
     1801 ggtaaagagt cgagccatta gattatcctc ggcttatgcg ttctgtctgt tcctctatac
     1861 gttctccatc attcccggta ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc
     1921 tgtgtcctga tcggtaacca gtgcgttgat ataaccgcct ttcattgcag cggcaattgc
     1981 ttcggcttta ttttctcccc ctgccacgcc aacccggacg ggtatggtct tcagcgagct
     2041 taaaggtaag ccaatcagtt cgttatgtat tttaatatcc gtgacaacgt caccttttgc
     2101 atcaaaaaag tagcctaaaa tgtcgccaac cgcccctttt cgaccaatca ttaactgttc
     2161 gccctggctg atataaccgg agcgaatgat cgtcgcgtcg tcctgttgac tgacggcccc
     2221 aatgccgaca atcgccacat cggctgcttg cgcggctaac agaacatctt tgacgcaatt
     2281 ttcatttttt agcgtacggg caatgtcagc ggaggatgcc cgcaacggag ccggaataat
     2341 attcacactg cacgccgcgt taagctgccc gatacccgtc atataagaac cgacgccacc
     2401 ggagagcgtg accaggcgaa tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt
     2461 attcatggtt gcctcgccaa aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat
     2521 caacatatgc gccgcgccta tccccagtcg cccgccgaca tcagcatccg caagcccagg
     2581 gatcacccgg acatgttgca gcgaaaactg acgacgtaat tgagtttcat attccagaca
     2641 gccttcaaag cgagaattaa tctgtacgcg aataatgccg gactgatgcc ctttctccag
     2701 caagcgcgac actttcaaac gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag
     2761 cccgtcgtga tagtaaaacc acgcgatccg cgcgacctgt tcttcttcac acattccctg
     2821 ttctgaaatt gccgaatcgt tgattgtcat aattcattct tcactttgaa catatttaaa
     2881 tctttaatgc aattgttcag ttcttgcgca tttatatctg tgatggcagc cacagtttga
     2941 ctctacgagc atgaacaaac gcaaccgtga aaatcaaaat agcataaatt gtgatctatt
     3001 cgtcggaaat atgtgcaatg tccacctaag gttatgaaca aattaaaagc agaaatacat
     3061 ttgttcaaaa ctcacctgca aaactgaacg ggggaaatat gcaaacgagt gatacccgcg
     3121 cgttaccgct actttgcgcc cgctcggttt ataaacagta ttcaggggtc aatgtcctga
     3181 aaggcatcga ttttacgttg catcaggggg aggtccacgc cctgctcggc ggcaatggtg
     3241 ccggtaaatc gacgttaatg aagattattg ccggtattac ccctgctgat agcggtacgc
     3301 tggagattgg gggcaacaac tacgccagat taacgccagt tcatgctcat cagctgggca
     3361 tttatctcgt tccccaggaa ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc
     3421 tgtttgggat ggcaaaaaaa cagctctcca tgcaaaaaat gaagaacttg ctggcggcgc
     3481 tgggctgcca gtttgatctg catagtctgg caggatcgct ggatgtcgcc gatcgccaaa
     3541 tggtggaaat cctccgcggg ctgatgcgcg actcgcggat tctgatcctc gatgaaccta
     3601 ccgcctcgct tacccctgcg gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg
     3661 ctactggcgt gggtattgtt tttatctcgc ataagctgcc ggaaattcgc cagattgccg
     3721 atcgaattag cgtgatgcgc gacggaacca tcgccttaag cggcaaaacc agcgaactgt
     3781 ctaccgacga cattattcag gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca
     3841 gccaaaaatt atggctggag ttacctggta accgcccaca acatgctgcc ggaacgccgg
     3901 tgctgacact ggaaaatctg accggcgaag gtttcaggaa tgtcagcctg acgctcaatg
     3961 ccggagaaat tctgggcctg gctgggctgg tgggagccgg acgcacagaa ctggccgaga
     4021 cgctctatgg tctgcgtact ttgcgtggcg gacgcattat gctgaatggt aaagagatca
     4081 ataaattatc cactggagaa cgtttactgc gcggtctggt ttatctgccg gaagatcgcc
     4141 agtcatccgg actgaatctc gatgcttcgc tggcctggaa cgtctgcgcc cttactcata
     4201 accttcgtgg attctgggcg aaaaccgcga aagataatgc caccctggaa cgttatcgtc
     4261 gggcgctgaa tattaaattt aaccaaccgg aacaagctgc acggacatta tccggtggca
     4321 accagcaaaa aatcctcatt gccaaatgcc tggaagcttc gccgcaagta ttgattgtcg
     4381 atgagccgac gcgcggcgtg gatgtctcgg cccgtaatga tatctaccag ctgttgcgca
     4441 gcatcgccgc acaaaatgtg gctgtgctgc ttatctcctc cgacctggaa gagatcgaac
     4501 tgatggcaga tcgtgtgtat gtgatgcatc agggcgaaat tgcccactct gcactgaccg
     4561 ggcgcgatat taatgtcgag actattatgc gcgttgcctt cggcgatagt cagcgtcagg
     4621 aggcatcatg ctgaagttta ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt
     4681 gctgctgttt gtattacccg gttttctcga ccgccagtat ttaagtgtgc aaacgctgac
     4741 catggtttat agcagcgcgc aaatcctgat cctgctggca atgggcgcga cgctggtaat
     4801 gcttacgcgc aatattgatg tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt
     4861 ggggatgtta ctgaacgcag gatattcact acctgttgcc tgtgtcgcga ctttactgct
     4921 tggtttgctc gcgggatttt tcaacggtgt cctggtcgcg tggctaaaga tccctgccat
     4981 tgttgccacc cttggcacgt tagggttgta cagaggcatc atgttgctgt ggactggcgg
     5041 taaatggatt gaagggttac ccgccgaact gaaacagctc tccgcaccgc tgctgtttgg
     5101 cgtttccgcg attggttggt tgacgataat tctggtggca tttatggcct ggctgctggc
     5161 aaagacggcg tttggacgca gtttttatgc cacgggcgat aatttacagg gcgctcgtca
     5221 actgggtgtt cgtacggaag ccattcgcat tgtggcattt tcgttgaacg gctgcatggc
     5281 ggcactggcg ggaattgtgt ttgcttcgca gattggtttt atccccaacc agaccggtac
     5341 cgggctggag atgaaagcaa ttgcagcctg cgtgctgggc ggcattagtt tgctcggcgg
     5401 ttccggtgcg atcatcggtg cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt
     5461 actggtgctg ttgcgcattc cggcatggtg gaacgatttt atcgcgggcc tcgtgctgct
     5521 ggcggtgctg gtgtttgatg gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca
     5581 aaaatatgcc cgctttatga cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa
     5641 tcgggaggcc gcataatgcg tattcgctac ggttgggaac tggctcttgc cgcactgctc
     5701 gttattgaga ttgtcgcatt tggtgcaatt aacccgcgga tgttagatct caatatgttg
     5761 ctgttcagca ccagtgactt tatctgcatt ggcattgtcg ccctaccgct gacgatggtg
     5821 attgtcagtg gcgggatcga tatttcgttt ggttcgacca tcggcctctg cgccattgca
     5881 ttgggcgtac tgtttcaaag tggtgtgccg atgccgctgg cgatactcct gaccttactg
     5941 ctcggcgcat tgtgcgggct gatcaacgcc ggattaatta tctataccaa agttaacccg
     6001 ctggtgatta cgcttggcac gctgtatctg tttgccggaa gcgctctgct gctttccggt
     6061 atggccggag cgacggggta cgaaggtatt ggtggattcc cgatggcgtt tacagatttc
     6121 gctaacctgg atgtgctggg actccccgtt ccgctgatta tcttcctgat atgtctcctc
     6181 gttttctggc tctggctgca taaaacccat gccggacgta atgtgttttt gattgggcaa
     6241 agcccgcgcg tggcgcttta tagcgcgatt ccagttaacc gcaccttatg tgcgctctat
     6301 gccatgacgg ggctggcgtc tgcggtcgcc gctgtgctgc tggtgtcgta ttttggttca
     6361 gcacgttccg atctcggtgc gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc
     6421 ggtgccaata tttatggtgg ttccggttcc attatcggca ccgccattgc ggttttatta
     6481 gtgggatatt tgcaacaagg tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc
     6541 ctttccggtg cgctacttat cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag
     6601 caaattaaag agtggctggc gcgtcgggcc aataacccat tgccataaag gatatcttca
     6661 tgacacttca tcgctttaag aaaatcgcct tacttagcgc tcttggcatt gccgcaatct
     6721 ctatgaatgt gcaggccgca gagcgtattg catttattcc caaactggtt ggcgtgggat
     6781 tttttaccag cggtggcaac ggcgcacaac aagcgggtaa agagctgagc gttgatgtga
     6841 cctacgacgg gccgacagaa cccagtgttt ctggtcaggt acagttgatt aataacttcg
     6901 tcaatcaagg ttataacgcc attatcgttt ctgcggttac gcctgatggc ttgtgtccgg
     6961 cactgaaacg cgccatgcaa cgtggtgtga gagtgctgac ctgggactct gatactaaac
     7021 cggagtgccg ctcttactac attaatcagg gaacgcccgc ccagttggga ggtatgttgg
     7081 tggatatggc ggcgcgtcag gtgaataaag acaaagccaa agtcgcgttt ttctactcaa
     7141 gccccaccgt tacggaccaa aaccagtggg tgaaagaagc gaaagcgaaa atcgccaaag
     7201 agcatcctgg ctgggaaatt gtcactacgc agtttggcta taacgatgcc actaaatcat
     7261 tacaaaccgc agaaggaata ttaaaagcgt atagcgatct cgacgccatt atcgcccccg
     7321 acgccaacgc cctgcccgct gccgcacaag ccgcagaaaa cctgaaaaat gacaaagttg
     7381 cgattgtcgg attcagtacg ccaaacgtga tgcgtccata tgtggaacgc ggcacggtga
     7441 aagaatttgg cctgtgggat gtggttcagc aaggcaaaat ttcagtgtat gtcgcggatg
     7501 cattattgaa aaaaggatca atgaaaacgg gcgacaagct ggatatccag ggcgtaggtc
     7561 aggtggaagt ctcgccaaac agcgttcagg gctatgacta cgaagcggat ggtaatggca
     7621 tcgtactgtt accggagcgc gtgatattca acaaagagaa tatcggcaaa tacgatttct
     7681 gatgtgcatt acttaaccgg agtaagttat ggcagattta gacgatatta aagatggtaa
     7741 agattttcgt accgatcaac cgcaacaaaa tatccctttt atcctgaaag gttgcggtgc
     7801 gctggattgg ggaatgcagt cacgcttatc gcggatattt aatccgaaaa cgggtaaaac
     7861 cgtgatgctg gcctttgacc atggttattt tcaggggccg actaccggac ttgaacgcat
     7921 tgatataaat atcgccccgc tgtttgaaca tgccgatgta ttaatgtgta cgcgcggcat
     7981 tttgcgcagc gtagttcccc cagcgaccaa taagccggtg gtactgcggg cgtcaggtgc
     8041 gaactctatt ctggcggaat taagtaatga agccgtggcg ttatcgatgg atgatgccgt
     8101 gcgcctgaac agttgcgcgg tggcggcgca ggtttatatc ggcagcgaat atgaacatca
     8161 gtcgatcaaa aatattattc agctggtcga tgccggaatg aaagtgggaa tgccgaccat
     8221 ggccgtgact ggcgtgggca aagatatggt gcgcgatcag cgttatttct cgctcgcgac
     8281 tcgaatcgcc gctgaaatgg gggcgcaaat tatcaaaacc tattatgtcg aaaaaggttt
     8341 tgaacggatt gttgccggat gtccggtacc cattgttatt gctggcggta aaaaattacc
     8401 ggagcgcgag gcgctggaaa tgtgctggca ggctatcgat cagggcgctt ctggtgtgga
     8461 tatggggcgt aatattttcc agtctgacca tccggtggcg atgatgaaag ccgtacaggc
     8521 ggtggttcac cataacgaaa cggctgatcg ggcatatgaa ctctatctga gtgaaaaaca
     8581 gtaactgcgg atttaaggag aagaattatg cacgtcacac tggttgaaat taacgttcat
     8641 gaagacaaga ttgacgagtt tatcgaagtt tttcgccaga accacctggg ctctgtacag
     8701 gaagaaggca atttgcgctt cgatgtctta caggacccgg aagtgaattc gcgcttttat
     8761 atctacgaag cctataaaga tgaagacgca gtggcattcc ataaaaccac gccccactac
     8821 aaaacctgtg tcgcgaaact ggaatcttta atgaccgggc cgcgtaaaaa acgtctgttc
     8881 aatgggttga tgccgtgagg cgaatttatc atttttatct acaattgggg taacgcgctg
     8941 acgggagtaa ataaatgtct gactggaacc cctctttata tctgcacttt gccgctgaac
     9001 gatcgcgtcc ggcggtggag ctgcttgcca gagtaccgct ggagaatatc gaatacgttg
     9061 ccgatcttgg ctgtggccca ggtaacagca ccgccctgct gcatcaacgt tggcctgcgg
     9121 ccaggataac aggcatcgac tcgtctccgg cgatgattgc tgaagcgcgc agtgctttgc
     9181 cagactgcca gttcgtggaa gcagacatcc gcaactggca accggaacag gcactcgatc
     9241 tgatttttgc taatgcctca ctgcaatggc tgcccgacca ctacgaattg tttcctcatc
     9301 tggtttcttt acttaatccg cagggcgtgc tggcagtaca gatgccagat aactggctgg
     9361 agccgaccca tgtgctcatg cgcgaagttg cctgggaaca aaactacccg gatcgcgggc
     9421 gtgagccgtt ggctggcgtt catgcttact acgatatttt gagcgaagcc ggatgtgagg
     9481 tcgatatctg gcgaaccacc tactatcacc agatgccgtc acaccaggcg attatcgatt
     9541 gggtgactgc cactggatta cgtccgtggt tacaggatct gaccgagagc gaacagcagc
     9601 tttttcttaa gcgctaccat cagatgctgg aagagcagta tccactgcaa gagaacggac
     9661 agatactgct ggcatttccg cgtcttttta ttgttgcccg gcgtatggag taaatatcac
     9721 gtcagctggt aatgacgatc gggaagaatt tttgctggaa tttcggcttc atcgttcatc
     9781 tgtaacaggt caatttcaat agcgttgcag atggcatcca gcggtaaatc attgttttca
     9841 gtaccgaacg gatcttccag ttcttccgcc agacaatcca gcgaaataaa agtgtaggaa
     9901 atcagcacag agataaaagg cgtcatgtaa tgcaggtcca cgaccagcgc gaacggcagc
     9961 atgatacaaa acagataaac ggtacgatgc aaaatcaggg tgtaggcaaa gggaattggc
    10021 gtataggcaa tgcgctcgca tcctgccagg accgctgaaa tatcattaag ccgatcgttg
    10081 aggctaataa acaggatatc tgaaagctgc ccattgcggc gctgaaccgc caaccactcc
    10141 cccattatta acaagatacg gttagctgga gagttcgaag ccagtacagg ctgaagatct
    10201 tcagtcttga gataatgagc cagcacttcc gcctgtggct gtttgcgtaa tgtcatgcgt
    10261 aaacagtggg cgaaggcgat ttgcaaccgg gcaaactccc ttacacttgc cgaatccggc
    10321 aatgtcgttt ttacctcgcg cagtaacgac cgtgaggcaa tcatcaactg cccccaaagt
    10381 tttcgcgctt caacgtaacg ggcgtacccg gcattattac gaaaaccaag aaaaatggcg
    10441 atggcgacac cgagaatgct gaacggtgcg agggtgaatt tgatgcccag atgcgtgtac
    10501 cagggcagca tgaaaataac agcgatagaa aagagaaaat tgagtagtaa gcgcgatgat
    10561 atcttggata atactgagcc gtgccagaca aaaatacggc gcagccagtg ttgttgtgga
    10621 cgaacaatca tggttatctt caggcgtgaa aaagtcgccc tattaaacgt gattacgatc
    10681 acattctcaa gacgctactt acaaattacc tacaagctta caacattaac aggcgctgca
    10741 tgtggcatct gttaatgata aaggcacata ggatgttgta actaatatag tgaaataaga
    10801 aacccggtcg aaaccgggtt cagaagtaac ggtgttatta gcacaacgga cgtacagctt
    10861 cgcgcatccc tttttcaaga atcgcatcca ggtcattagc aacctgctcg accagaccag
    10921 gcacttgcgt caggtcctgc tcccagtggt ctttttctgc caacacaatc gctaccagtt
    10981 cctgggtacc gataacacgg tcacgatgct ggctccagag ttgctgataa cgttccagcc
    11041 agtgtgcatc gtcctgtacc ggatatgttt ctccgttacg ctcaccacga tagaaggcaa
    11101 tcaatgccgc taatgcgaaa gtaaggcgcg ccggaagtgt gccttttgcc ttctgccctg
    11161 ccagcagctg cggcaggatg cgagtgcgga acttggtcat accgttgagc gcgatagaca
    11221 gcagctgatg cttaatgtac gggttacgga aacgcccggt gactgcactg gcgaaagatt
    11281 ccagttcatc acgaggcaaa tccagtaccg gaataatttc ttcgtagata gctttttcaa
    11341 cgaatgcgca aatttcagca tcgttcatcg cttcacctac ggtatccagc cctgcctgga
    11401 acgccaccgg caccagcgcg gtgtgcgcac cgttgaggat tgccactttg cgttctttgt
    11461 acggtttaat atcgtcaaca atcagcacgt tgagcggata tttgtccaga cgcagttcag
    11521 tcgctaagga tttcggcccc tgaatcacaa acaggtaaaa gtgttcagcg gtgtcgagga
    11581 aaccatcgtg ataacccagt tcttcttcca gtttagccac ttcatcgcgc ggataaccgg
    11641 taacgatacg gtccaccagc gtagaacaga agctgttagc ctgatccaac cattgaataa
    11701 atgcttctgg cagtgcccac tcttgagcat agcgcagcac cagttcacgc aacgcgtcgc
    11761 cattgtagtc gatcaactca cacgggatga tgatccaacc tttatccagc gcaccgttga
    11821 aatggctaaa acgttcgaac agcagacggg tcagttttgc gggatagctt accgctggcg
    11881 cgtcatcgaa tttatcgccc gcatggtagc tgatacccgc ttcggtggtg ttagagaaaa
    11941 caaagcgcat ttccggattg tgcgccagtt tcaggaattc atcgtattca ctgtagacgc
    12001 tgatttcacg attaacagag cgaatcagac gcgcgtcgct gaccgcttcc cccttctcat
    12061 tcaggccacg gataatggtg gtgtacagac catcctgcgt gctcagtgac ggcgggaatg
    12121 aagtttcaat cggacgaaca acgaccacgc cagaattcag atcggtgtgc tcattcagga
    12181 gatcgatttg ccagtcaaca aaggcgcgca ggaagttacc ttcaccaaac tgaatgatac
    12241 gttctggata ctgtgcaccg ggaaaatcgc gacgatttag tgttttcaca atgggttccc
    12301 ttctgattag tcatacaacc tgcttgaatt ggtacgacag gttagcaaac tttaatacgc
    12361 cgaacccctg ttttgatcaa ctcctgatga ttaatgagca gttttatgag aaaagtgtgg
    12421 cgcggatcat ggtttaatcg aggaaatacg ccttttcctg gatcataaag tggtagaaca
    12481 cattgcattc aaatcgcgcg gaatgaataa agatgtcaga caacttcctc accgtaacgc
    12541 atagtgctgg tacggttgcg cccatctttc ttcgagcgat acagacacgt atcggcttcg
    12601 accatcaatt tattaaaatc atcggtcagc gttcggtacg atgcgcgacc actaccgacg
    12661 ccaatactta ccgtgagata aagcgttttt tgttgccagg tgaatggttg cagttcaacg
    12721 cctttacgga ttttttccgc cattagcaga ccatcgacag gattcaccga cggcactgca
    12781 acagcaaatt cttcgccgcc catgcgcgcc accagcccct tatcaccgac aatcttctga
    12841 atatgccggg caaacacgct taacacttta tcgccacatt catgtccata gttatcgtta
    12901 atgcttttga aatagtcgat atcaagcagc atgacagtca gatgttgtgt ctgtttcagc
    12961 gatggacttt tcagcgcctc ataaagaccg gaccgtgagt aaacctgagt cagaaaatca
    13021 aagtcggctc gcagcgcaac ttgcttcatt agcgaattga tcgctgccac gctaaaagaa
    13081 accataattg ggcatatcgc catcgtggca ataccgagac gtgcggagaa catttgcgga
    13141 atggagaacg gcgaaccgac cgagatatca atcaccgaat ttgccaccag tacgatttcc
    13201 accgcaccgg tgacaaaggt caacagacat gttacctgcg gcgtatagcg cactgcacac
    13261 cagattaatg caggcagcgg gaacgccaga ctccccgctc cgccaatgac taccgaggca
    13321 ataaccgaca caataagcgc gatggcgggc atcatctgtt ctgctttaaa gcgcggcagc
    13381 actccaggaa tagccaacgt cagcatacaa ggcacgatca aaacgcccgt tgagaattgc
    13441 tcactgaacc agtccgcaag caaaggccag aaatccagac tatcaatact gaccgaacca
    13501 atcgccccga caatagcgca taataatgcg gcaatcagac agtaattaaa tagccgtaag
    13561 gcacttactg gctcatactt atttttcccc aaacgcttat cccgcacgac cagtaaggcg
    13621 acggtaacaa taaacatcat attggagaaa ttgatagcca gtgaaaccag tccccattcg
    13681 gtggttatgg catcataaac cagcatcgcc acataactga tcgcatagta atgcagtcga
    13741 ttaagccaga catagcgggc aaacaccccc gccattacgc cattcagcgg ccagaaaaga
    13801 gaaagttcgt gaaccagccg taactctgca ccaataaaat agaacagcgt ggttagcgca
    13861 aaaatagcga tgctattacg tagcagatga ccttcctgga acaaacggaa agttgaaatg
    13921 gggtgtacat gcattaatta tctgtccata caattgcggg tggcaattat ctcaacgtaa
    13981 taagtaaatt ctcataaagg atagcatgtt cgctgacagc ggcggcaaat aaaggtaccc
    14041 tgggaatgct attttatcgt tttccgcaat tctcatggct gattcatctg tacaaccagc
    14101 cagtgataga ttaccgccac gatataacgt tgttgttctt cgcttaacga aacaccctgc
    14161 ggaatgttat gccacttagc cagacaaccg cgacaacagg ttgcagtggc gtgctgagcg
    14221 ataaacactg gatgcccgcg catgggggtt tgcttaccgt cattagccgg taatgccgga
    14281 gcaagacgtt tagcgacaaa atccgccgca tgttgttcga tgactggcgc gcctttctcc
    14341 agacaatact gacgctcttt catgcccaaa cgaaagcgag atctaaattt tgaacgggat
    14401 aaacgcgcga agagtggatc gagagactgc attaataaac cgaacgccct aactgtttcg
    14461 ttaattgttc aagaacggca atgcccgcaa gcgagttacc tgcatcatcc agttccggac
    14521 tccagactgc gatggccatt tcatgcggaa caatcgccac aataccgcca ccaacgccag
    14581 atttcgccgg tagtcccacc cgccaggcaa actcccccgc gttctggtac ataccactgg
    14641 tcgccatcag cgcgttaatt tgccgcgcct gcattggcgt caccacaggt tcatcaatat
    14701 gaatagcttt cccctgatta gccagaaaga caaacgtccg ggccagctct acacagctca
    14761 ttttcagagc gcagtaatga aagtagtttt gcagaacggt tgtcacgtca tgatggaaat
    14821 tgccaaacga cttcatcagc caggcgatag ccgcatttcg cgcggaatgt tcaaattcgg
    14881 aacgcgctac caccgtatcg tatgaaatat cagacacacc gcttaagccg cgcacgactt
    14941 ccagcatacg ttgccgtggt gcgcttagtc gcccttgcaa catatcgcag accaccagcg
    15001 caccggcatt aatgaacgga ttacgcggaa taccctgttc catttccagt tgcactaaag
    15061 aattgaacgg tgatccagac ggatctttgc cgacgcgttg ccagatttcc tcttcggagt
    15121 aatgacgcat ggcgacaacg agactcagca ctttggaaat agactgaatg gaaaaacgtt
    15181 cttgcgcgtc tccggcctga aaaagctgtc cgtcaacggt acagatagca atgcccaatc
    15241 gggaaccgtc tactgtagcc agcgccggaa tataatccgc gactttaccc tgaccaatga
    15301 gcggccgcac ttgccgcaag atgttttcta aaattgcatt atccatggcg actgccactt
    15361 tctactcctg gaccgcaggt ctgaaaagac ctgcgagtat atcagagctg aatatgtcgc
    15421 gtcagatccg gtctttccac accgtctgga tattacagaa ttcgtgtaag ccgaaatggg
    15481 aaagctcacg accaaagcca ctctttttca cgccaccaaa ggccactcgc gcgtcgctgg
    15541 cacaataacc attgataaac accccaccgc attccagacg tgccgccatc tgtctggcct
    15601 gtgtttcgtc ggtggtaaaa atggtcgctg aaaggccgaa ctcactatca ttagccagtt
    15661 ccagcgcatg ttctgcatct ttcgcaacgg tgattgccgc aacagggcca aacatttctt
    15721 cccgaaacgc ggtcatttct ggggtaacat tcgccagaac cgttggcgga tagtagttac
    15781 ctgccccagc catcttttcc ccgcccagta acaaacgcgc accctgcgcc agggttttct
    15841 ccacctgatg atgcagctca tcacgtaaat caaaacgagc cattggtccg agagcattct
    15901 cttcatcacg gggatcgccc attttcaagg ctgccgcagc tgccacaaaa cgttcggtaa
    15961 atgcagaagc aattccctct tcgataataa agcgttttgc cgctgcacaa acctgtccgg
    16021 tattctgata acgtccggct accgccgctt taaccgccag ttccagatcg gcatcgttaa
    16081 gcacaataaa tggatccgaa ccgcccagtt ccagtacgca ttttttcagt gccgctccag
    16141 cctgtgcgcc aatagccgct cccgcacgaa cacttccggt caccgtgaca gcagcaatgc
    16201 gcgaatcttt aatcatttga ctgacaccgt cgttgtcggc attcagccag ccatatacgc
    16261 cttgcgggat tcccgcatct ttaaacacct gggcaatgag ctgagcacag cccatcacat
    16321 tcggcgcatg tttaagtaag tagccgttac ctgcaagaat gatgggaacc gcgccacgca
    16381 tcacctgcca taacggaaag ttccacggca taatcgccag aatcgtcccc aacggtcgat
    16441 actcaataac tgcctgctga ttttccacca gcgtaggttc cgccttcagc attgccggac
    16501 catgttctgc ataccagtca cacaaattcg ccgatttcgc cacttcagcg cgcgcctgat
    16561 tgattggttt gcccatttca cgggtgatca tttgcgccat ttcttcgcta cgggcgcgca
    16621 gagccttacc gataccacgc agtttttcag cacgataatc tatatttgtc tcgcgccagt
    16681 cgcgaaagcc tgctgccgcc agctgaagtg cgttttcgat atcgttagcg ccagcccaag
    16741 gcagcacaga aagttgttca cccgtggcag gatttattga aattgcatga gttgccggag
    16801 taatggtcat cggggtatct cctttatgag tcatggtatg aagatacgca gatttactct
    16861 tgctttaaaa tgaataatat taagccactt attcacgaat cgagaatgtt atggatctga
    16921 cccaactgga gatgttcaac gcggttgccg aagctggcag cattacccag gctgcagcaa
    16981 aagtgcatcg cgtgccgtcg aatctcacta cccgtctacg ccagctggaa acagaactgg
    17041 gggtagatct gtttattcgc gagaatcagc gtttacgtct ctctcctgcc gggcataact
    17101 ttttacgcta tagccaacaa attctcgcgt tagtggatga agcgcggagc gttgtcgctg
    17161 gcgatgagcc gcaaggttta ttttctcttg gttcgctgga aagcaccgca gcagtgcgca
    17221 ttccggccac gctggcggaa ttcaaccatc gttatcccaa aattcagttt tcactttcca
    17281 ccggcccttc cggcactatg ctggaaggag tgctggaggg aaaactgaat gcggcattta
    17341 ttgatgggcc cattaaccat actgccattg acgggatacc ggtataccgc gaggagctga
    17401 tgatcgtcac gccacaaggt catgcgccag taatccgcgc cagtcaggtt aacggcagta
    17461 acatttatgc cttccgcgcc aattgttcgt atcgtcgcca cttcgagagc tggtttcatg
    17521 ctgacggtgc cgctccggga actattcacg agatggagtc gtatcacggg atgttggcct
    17581 gtgtggtcgc aggagcaggc attgcgctta ttccacgctc tatgctggaa agtatgccgg
    17641 ggcatcacca ggttgaagcg tggccgttag ctgagcaatg gcgttggtta acaacctggc
    17701 tggtctggcg tcgtggtgcg aaaacgcgtc cgcttgaggc atttattcaa ctactggatg
    17761 tgcctgactc ggcaaaacag ggttaccaat gagctatttt tgatagttct tgcagctcaa
    17821 atgccctata tagtgatgtt ccgatgactt atgactatat ggggcaaata tggttacgcc
    17881 agtaagcatc agcaattata tatctcttcc tgatgatttt cccgctcgta atattgcacc
    17941 tcaggtaaaa gaagttttaa aggattttat tgatgcactc agtaccataa tatgtgatga
    18001 agagtggcgc acgtctttaa acatcaactc agccacaaaa aagatattta ataaccttga
    18061 taacttatca tatattcaga ggac
//
LOCUS       DAZGVQ010000103        18065 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000103 DAZGVQ010000000
VERSION     DAZGVQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.104"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..385
                     /gene="waaH"
                     /locus_tag="ACHLAG_003394"
     CDS             <1..385
                     /gene="waaH"
                     /locus_tag="ACHLAG_003394"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFP0656560.1"
                     /translation="HNTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYP
                     KFHQQITYEALRVCHAVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLW
                     SFRLWQWRDKTRSHHRITRSAFNLR"
     gene            complement(372..1331)
                     /locus_tag="ACHLAG_003395"
     CDS             complement(372..1331)
                     /locus_tag="ACHLAG_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFP0656561.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(1335..2594)
                     /gene="envC"
                     /locus_tag="ACHLAG_003396"
     CDS             complement(1335..2594)
                     /gene="envC"
                     /locus_tag="ACHLAG_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFP0656562.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            complement(2628..4172)
                     /gene="gpmM"
                     /locus_tag="ACHLAG_003397"
                     /gene_synonym="pgmI"
     CDS             complement(2628..4172)
                     /gene="gpmM"
                     /locus_tag="ACHLAG_003397"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFP0656563.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            4416..4847
                     /locus_tag="ACHLAG_003398"
     CDS             4416..4847
                     /locus_tag="ACHLAG_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP0656564.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVISRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            4989..5240
                     /gene="grxC"
                     /locus_tag="ACHLAG_003399"
     CDS             4989..5240
                     /gene="grxC"
                     /locus_tag="ACHLAG_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFP0656565.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            5303..5770
                     /gene="secB"
                     /locus_tag="ACHLAG_003400"
     CDS             5303..5770
                     /gene="secB"
                     /locus_tag="ACHLAG_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFP0656566.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            5770..6789
                     /gene="gpsA"
                     /locus_tag="ACHLAG_003401"
     CDS             5770..6789
                     /gene="gpsA"
                     /locus_tag="ACHLAG_003401"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP0656567.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            6869..7690
                     /gene="cysE"
                     /locus_tag="ACHLAG_003402"
     CDS             6869..7690
                     /gene="cysE"
                     /locus_tag="ACHLAG_003402"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFP0656568.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(7743..8216)
                     /gene="trmL"
                     /locus_tag="ACHLAG_003403"
     CDS             complement(7743..8216)
                     /gene="trmL"
                     /locus_tag="ACHLAG_003403"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFP0656569.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(8371..9561)
                     /gene="lldD"
                     /locus_tag="ACHLAG_003404"
     CDS             complement(8371..9561)
                     /gene="lldD"
                     /locus_tag="ACHLAG_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFP0656570.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKEFPAALAPMAK
                     GNAA"
     gene            complement(9558..10334)
                     /gene="lldR"
                     /locus_tag="ACHLAG_003405"
     CDS             complement(9558..10334)
                     /gene="lldR"
                     /locus_tag="ACHLAG_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFP0656571.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            complement(10334..11990)
                     /gene="lldP"
                     /locus_tag="ACHLAG_003406"
                     /pseudo
     CDS             complement(10334..11990)
                     /gene="lldP"
                     /locus_tag="ACHLAG_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
     gene            complement(12358..17208)
                     /gene="ehaG"
                     /locus_tag="ACHLAG_003407"
     CDS             complement(12358..17208)
                     /gene="ehaG"
                     /locus_tag="ACHLAG_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFP0656572.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVATEENTISVGSS
                     TNQRRITNVAAGKNDTDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            complement(17252..17935)
                     /locus_tag="ACHLAG_003408"
     CDS             complement(17252..17935)
                     /locus_tag="ACHLAG_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP0656573.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
BASE COUNT         4144 a         4746 c         4651 g         4524 t
ORIGIN      
        1 gcataatacg tcagtgagtc ggttgcatag acaagggaat aaaaacctta attatcaacg
       61 tcactatatt aagattaccc gcctgctgga gaaattaaat cgaaattatg ccgacaaaat
      121 tacgatttat ccgaaatttc atcagcaaat aacttacgaa gcattgcgtg tttgccatgc
      181 ggtgcgcaaa gagccggata ttcttacccg ccaacggatg attgccgaga tatttacttc
      241 cggtatgtat aagcgcctga ttaccaatgt gcgcagcgtg aaggtcggtt accaggcgtt
      301 actgtggtct ttccgcttat ggcaatggcg cgacaaaacg cggtcgcacc atcgcattac
      361 gcgtagcgcc tttaatttgc gctagcgtta aacttcgccg cttcgggctg tttgccccag
      421 ccttgccact gatgctggaa gtaaacgatc agcgtgctct ggctgatgct ttcgcttaat
      481 acttcaaaga agcgattagc ataaacaggt tctatcggtt tcttcggttt gcacagcttc
      541 acgccacgga acggattacg cggtgcatca ggcacagcgt ttttcggcgg tgtagaagta
      601 tcaacctgcg gttcattcag caagctgctg gctttcacca gcgtaatgtc tggcggcagg
      661 ttataaacca tctgttgtag cacgcgtacc gttgaagggt gaggatgccc aatggcaatg
      721 gttgaaccgt tgcgacgcgc cagatcaatt gcgcgattaa attgcacacg gatgtccgct
      781 tcgttctgcg aatcgtcgag gaacaccttc cgtttgatca ccttcacgcc agtaccttgc
      841 gcggcgcgca tcgcctgggt attaccgatg gttacgctgt cgaggaagta aagattgtaa
      901 cgctccagtg cctgcatcac tttctgcata ccaaacaggt tagaggtcat cttgctaccc
      961 atgtggttgt tgatccccac ggcatagggc acgttattga ccgcactacg aataatgcgc
     1021 tcaatttcgt cgctgctcat ctccgggcgc agcgtatttt tctccagcgg ctgtttactc
     1081 agtggtgcca tcgggagatg aatcaacact tcgtgcccgc tgttatgcgc tttggtcgcc
     1141 atctctctgg cgtgcggtga atcgggtaat acagcgacgg agatagcgga gggcatcgcc
     1201 agcacctggt tttcgttgtg cgggcgatac ccaaaatcat caatgacgat ggcaagtttg
     1261 ccagcaagta cgggggagga gagcgccaac agagcggcaa atgcaagaac gttacgacga
     1321 aatggaaaca aaacttatct tcccaaccac ggctgtggat tgaccgcctg accctggcgg
     1381 cgaatttcga aatagagtga aggccgaccc tgaccgccac tgctgcccac cagtgcaatc
     1441 ggctggcccg cgcgaacctg cgaaccaacg ctcaccagcg cgctctgatt atagccgtaa
     1501 agactcatgt cgcctttacc atgctcaacc accaccacca agccgtaacc ttgcagccag
     1561 tcagccagaa tcacccgacc gtcggcaatc gctttaactt cagtaccttc agaggcacca
     1621 atcaccatcc ctttccagcg taattcaccc tgtagctgct cgccatagcg atgcagcgtc
     1681 ggcccgcgaa ccggccagaa tgcctgacca cgcggcgcac ccagaccacc ggtacgggac
     1741 atcagcgatt tttcgctttc ggtcggttta taggtggtgc ctttgcgcgt cgcttctttc
     1801 tggcggtcac gaaccgcctg ggcctcacgt gcttctcgtt ctgcacgcgc tttcgccgcg
     1861 gcttccgcac gggcaatgct gttacgcaga cgggattcgt tggcacgcag ttcacttagc
     1921 tgctgctgac cttgctggat ggaagattcc agccccgcca gcgtcttttt acgctcgttc
     1981 agcgcctgag tcagtttcgc ctgttgggcg cgctgctcat ataacagcgt ttgttgctcg
     2041 ctctgtttct cttccagttc agcacgctgc atggcgactt cttcacgcgt ttgcttcaac
     2101 tgggcaatgg tttcttgtcg cgcctggttg agatagccga aataagcctg taaacgctgt
     2161 ccacgctggc tttcttcacc gctgagaatc agctgaatac cggtatgctc gccctgacgg
     2221 aatgcggcat ccagttgtgc ggcgaggctg cgctcctggg cggctttttg ctgctccagt
     2281 ttggcaatcg acgcgttcat ctcatcaatc tgtttattga gttgattgag cgtgttttgc
     2341 gtttcgcgca gcttacgggt ggcttcagag atcgcttctt cctgcttttt caattgtgcg
     2401 agcaggcttg cgcgttgttg ttgcttttgg cgtaccgcgc gctctttcgc ggcgatatcg
     2461 gcctgaatag atttgagttg gtcacgctca tccgcgtggg cggaaaaggc gcacaacaat
     2521 acgccagcgc taagaacgct ggcgtagatg atgggcctga ttgcaaacct gcgcggtttc
     2581 acggcccgtg tcatggtatt aatcgccttt cccctcatgg ggagggatta ttccacgatg
     2641 aacagcggct taccagtcat ctcttgcggg atttccatac ccatcagcga caacatggtc
     2701 ggcgcgatgt cagaaagttt gccgccttca accgctttca cgttcttatc gccaacgtaa
     2761 atcagcggaa ctggcaggtt ggtgtgtgcc gtgtgtgcct gacccgttgc cggatcgcgc
     2821 atctgttcag cgttaccgtg gtcagcggtg atcagcagtt gtccacccac ggactcaacc
     2881 gctttcgcga cttcttccac gcagtgatcc agcgcttcaa ccgctttaac cgctgcttcc
     2941 atcaccccgg tgtgacctac catgtcgccg ttcggatagt tacagataat tgtgtcgtat
     3001 ttgccgctct tgattgccgc aaccagtttt tcggtcagct ctgcggagct catttccggt
     3061 tgcagatcgt aggtagccac tttcggtgag ttgatcagaa tgcgatcttc gcctttgaac
     3121 gactcttcta cgccaccgtt gaagaagaaa gtaacgtggg catatttttc ggtttcggaa
     3181 atacgcaact gagttttgtc gtttttcgcc atccactcgc cgaaggtgtt aaccagggaa
     3241 gcgggtgggt aagcaaccgc agttttgatg tcagcggcgt attcggtcag catcacgaaa
     3301 tcgacgttaa ccactttctt acgcgcgaag ccatcgaaat cagcgttcac gaatgcacga
     3361 gtgatttcac gcgcgcggtc agcacggaag ttcatgaaaa tcagcgcatc gccgtcttcc
     3421 attgccgcat ctggctgacc ttctgcacga ataacggtcg ctttcacgaa ctcgtcgttt
     3481 tcgtcgcgag cataagctgc ctgcaaacca gcaacggcag tatcggcctg gaactcaccc
     3541 tgcgccagag tcagcaggtc ataggctttt tcaacgcgat cccaacggtt atcgcggtcc
     3601 atcgcgtagt aacgaccaat gatggacgct acgcggcctt tacccagcgc agcaaatttc
     3661 tcttcaaatt ttttcagcga ggattcagca ctgcgcggcg gggtgtcgcg accgtcaagg
     3721 aatgcgtgca ggtagatttt ttctgcgccg cgttcagctg ccagttctac catcgccatg
     3781 atgtgatctt cgtggctgtg tacgccgcct gcggagagca gacccataat gtgtactgct
     3841 ttacctgcgt ttttcgcttt atctaccgcg ccagtcagca ctggattagc aaagaaagcg
     3901 cgatctttga tttcaacgtc cagacgagtc aggtcctgat acacgatgcg gccggcaccc
     3961 aggttaacgt ggcctacttc ggagttaccc atctgacggt caggcagacc gacttccaga
     4021 ccggaagcgt cgatcagggt atgcggacga ttggcccaca gtgcatccat taccggggtt
     4081 ttagcactaa aaatggcgtt atcctgctgt tcttcacgat agccatagcc atccagaatc
     4141 accagtacca taggtttttt agaaaccgac attgcgacaa cctcatactc aagagtcaaa
     4201 atttgcgtaa ttttactaca gctgaatcga taaaatagcc tctgaagatc aaaatatacg
     4261 accgctcgcg gcaagccgat gccagatttg cgcttttttt cgtcgtgccc cgcaaaattg
     4321 cattccagtt aacgcgctgg ctgtatttgc cgcaccgcgc aggtatactc ctttcctggt
     4381 ttttttaatc actacgtcgg gagttgttac cccccatgca agaaattatg caatttgttg
     4441 gccgtcatcc catactgagt atcgcctgga tcgcgttact ggtggcggtt cttgtgacta
     4501 cgtttaagag cctgacctcg aaagtgaagg tgattagtcg tggtgaagct acgcgtctga
     4561 tcaacaaaga agacgctgtg gttgtggatt tacgtcagcg tgatgacttc cgtaaaggcc
     4621 atatcgcagg ttctatcaat ctgttgccga gtgaaatcaa agccaacaat gttggtgagc
     4681 ttgagaagca caaagacaaa ccggttatcg tggtagacgg ttctggcatg cagtgccagg
     4741 agcctgcaaa cgcgctgacg aaagctggtt ttgcgcaagt attcgtgctg aaagagggcg
     4801 ttgctggctg ggctggcgaa aacttgcctc tggtgcgcgg caaataattt acctgtcgtc
     4861 tttcatgatg caggccgtta gctgcaatta ggcccccagc gacttattgt tataagcttc
     4921 tggggacact ccattttgca gccgccctgc aacatgaaat ttcagggtaa aaaaacagga
     4981 gctaaattat ggccaatgtt gaaatctata ccaaagaaac ctgcccgtat tgccatcgtg
     5041 caaaagcact gctgagcagc aagggcgtga gttttcagga gttgccgatc gatggcaatg
     5101 ccgccaagcg tgaagagatg atcaaacgca gcggtcgcac cacggttccc cagattttta
     5161 ttgacgcaca gcacattggc ggctgtgatg acttgtatgc attggatgca cgtggtggac
     5221 tggatcccct gctgaaataa cgtgtgaacg ttggcattac attgcgcagt atttaaggac
     5281 aacacttaag ggttttctac acatgtcaga acaaaacaac actgaaatga ctttccagat
     5341 ccaacgtatt tataccaagg atatctcttt cgaagcgccg aacgcgccgc acgttttcca
     5401 gaaagattgg caaccagaag ttaaacttga tctggatacg gcatcttccc aactggcaga
     5461 tgacgtatac gaagtggtac tgcgtgttac cgtaacggcc tccctgggcg aagaaaccgc
     5521 gttcctgtgt gaagttcagc agggcggtat tttctccatc gcgggtatcg aaggcaccca
     5581 gatggcacat tgcctgggcg catactgccc gaacattctg ttcccgtatg ctcgtgagtg
     5641 catcaccagc atggtatccc gcggtacatt cccgcaactg aaccttgcgc cggttaactt
     5701 cgatgcgctg ttcatgaact atttgcagca gcaggctggc gaaggtactg aagaacatca
     5761 ggatgcctga tgaaccaacg taatgcttca atgactgtga tcggtgccgg ctcgtacggc
     5821 accgctcttg ccattaccct ggcaagaaat ggccacgagg ttgtcctctg gggccatgac
     5881 cctgaacata tcgcaacgct tgaacgcgac cgctgtaacg ccgcgtttct ccccgatgtg
     5941 ccttttcccg atacgctcca tcttgaaagc gatctcgcca ctgcgctggc agccagccgt
     6001 aatattctcg tcgtcgtacc cagccatgtc tttggtgaag tgctgcgcca gattaaaccg
     6061 ctgatgcgtc ctgatgcgcg tctggtgtgg gcgaccaaag ggctggaagc ggaaaccggg
     6121 cgtctgttac aggacgtggc ccgcgaggcg ttaggcgatc aaattccgct ggcggttatc
     6181 tctggcccaa cgtttgcgaa agaactggcg gcaggtttac cgacagctat ttcgctggcc
     6241 tcgaccgatc agacctttgc cgatgatctc cagcagctgc tgcactgcgg caaaagtttc
     6301 cgcgtttaca gcaatccgga tttcattggc gtgcagcttg gcggcgcggt gaaaaacgtc
     6361 attgccattg gtgcggggat gtccgacggt atcggttttg gtgcgaatgc gcgtacggcg
     6421 ctgatcaccc gtgggctggc tgaaatgtcg cgtcttggcg cggcgctggg tgccgatcct
     6481 gccacgttta tgggcatggc cgggctgggc gatctggtgc ttacctgtac cgacaaccag
     6541 tcgcgtaacc gccgttttgg catgatgctc ggtcagggca tggatgtaca aagcgcgcag
     6601 gagaagattg gtcaggtggt ggaaggctac cgcaatacga aagaagtccg cgaactggcg
     6661 catcgcttcg gcgttgaaat gccaataacc gaggaaattt atcaagtatt atattgcgga
     6721 aaaaacgcgc gcgaggcagc attgacgtta ttaggtcgtg cacgcaagga cgagcgcagc
     6781 agccactaac cccagggaac ctttgttacc gctatgaccc ggcccgcgca gaacgggccg
     6841 gtcattatct catcgtgtgg agtaagcaat gtcgtgtgaa gaactggaaa ttgtctggaa
     6901 caatattaaa gccgaagcca gaacgctggc ggactgtgag ccaatgctgg ccagttttta
     6961 ccacgcgacg ctactcaagc acgaaaacct tggtagtgca ctgagctaca tgctggcgaa
     7021 caagctgtca tcgccaatta tgcctgctat tgctatccgt gaagtggtgg aagaagccta
     7081 cgccgctgac ccggaaatga tcgcctctgc ggcctgtgat attcaggcgg tgcgtacccg
     7141 cgacccggca gtcgataaat actcaacccc gttgttatac ctgaagggtt ttcatgcctt
     7201 gcaggcctat cgcatcggtc actggttgtg gaatcagggg cgtcgcgcat tggcaatctt
     7261 tctgcaaaac caggtttctg tgacgttcca ggtcgatatt cacccggcag caaaaattgg
     7321 ccgtggcatt atgctcgacc acgccacggg catcgttgtg ggtgaaacgg cggtaattga
     7381 aaacgacgta tcgattctgc aatctgtgac gcttggcggt acgggtaaat ctggtggtga
     7441 ccgtcacccg aaaattcgtg aaggtgtgat gattggcgcg ggcgcgaaaa tcctcggcaa
     7501 tattgaagtt ggtcgcggcg cgaagattgg cgcaggttcc gtggtgctgc aaccggtgcc
     7561 gccgcataca accgccgctg gcgttccggc tcgtattgtc ggtaaaccag acagcgataa
     7621 gccatcaatg gatatggacc agcatttcaa cggtattaac catacatttg agtatgggga
     7681 tgggatctga tgtgctgtga tcgtgccgga tgcgatgtaa tcatctatcc ggcatacagt
     7741 aactaatctc tcaacaacgc tcccggatat cccagctgcc tccaggcttc atacaccaca
     7801 actgacaccg cattggacag gttcatgctg cggctgtccg gcaccatcgg aatgcgaatt
     7861 ttttgttcag cgggcagggc atcaagaatg ctcgctggca ggccgcgtgt ttccgggccg
     7921 aacatcagat aatcgccatc ctgatagttt acggcgctgt gagcaggcgt acctttcgtg
     7981 gtgagggcaa acaggcgctg gggattttct gcttcgagga acgcgcgaaa gtcatgatga
     8041 cgcgtaacgg cggtaaactc gtgatagtcc aaccccgcgc ggcgcaggcg cttatcgtcc
     8101 caggcaaatc ccatcggttc gatgatatgc agacgaaagc cggtattagc gcaaagacgg
     8161 atgatgttgc cagtatttgg cggaatttct ggttcgtaaa gtacgatgtt tagcatactg
     8221 cccccttagt gcgggggcag gatagcagaa aattggtgag cagtgcggcc ttttcccctc
     8281 accccggccc tctccctgtc tcttgatcag gagatctgat caagagacag ctccctgagg
     8341 gagagggtta gggggagggg gcgcaaacga ctatgccgca ttccctttcg ccatcggagc
     8401 cagtgccgca ggaaactctt tacccagccc ctgcaccagc gaatcttgcg taatttcgct
     8461 gatcgatttc gcgccagtca gcgtcatcgc cactttcatc tctttctcga tcagatttag
     8521 caggttagct acacccgcct ggcccgctgt tgccagcgca tacaggaaag cacgacccag
     8581 cagtacggtg tcggcaccga gcgcaatcat acgcacgaca tcaagcccgt tacgaattcc
     8641 gctatccgcc agaatggcga tatcaccttt caccgcatct gcaatagcgg gcaacgcacg
     8701 agcggaagag agtacaccgt ccagctggcg gccaccgtgg ttagaaacca caattccatc
     8761 agcaccaaaa cgtactgcat cgcgcgcatc ttccggatcg aggatccctt tgatcaccat
     8821 cgggccatcc cagaaatcgc ggatccactc aaggtctttc catgagatgg acggatcgaa
     8881 gttattcccc agccagccga tgtaatcttc cagtccggtc ggtttgccga gataagctga
     8941 gatattacct aaatcatgtg gacgaccgtt caggcccaca tcccacgccc attgcggatg
     9001 cgtcaccgcc tgcaagtagc ggcgcattgc cgcatttggg ccgctcatac ctgaatgcgc
     9061 atcacggtag cgtgcgcccg gtgtcggcat atccacggtg aaaaccagcg tcgaacaacc
     9121 cgctgctttt gctcgctcca gcgcgttacg cataaagccg cgatcgcgca gtacataaag
     9181 ctggaaccac attgggcgct tgatggctgg cgcgacttct tcaatcgggc aaacggaaac
     9241 cgtcgagaga gtaaacggaa tgccatgcgc gtccgccgct ttggctgcct gaacttcgcc
     9301 acgacgcgca tacatgccac acaaacccac cggtgccagt gctaccggca tcgacaattt
     9361 ctcattaaac agcgtcgttt ccaggcttaa gtcggacatg tttttcagaa tacgctggcg
     9421 cagtgccact tctgacaaat cttccacgtt gcggcgcagc gtgtattcag aatatgcacc
     9481 accatccata tagtggaaca ggaacggcgg cagaatgcgt tgcgctgcgg cgcgataatc
     9541 gctggctgcg gaaataatca tgcgtttttc tccctcgaat gctcattatg ctcaccgggc
     9601 aggcgggtaa tccgtgcgtg gcgagcctga tcttcatcga atcgtttcat ggtggtgtga
     9661 acaaaactaa ggtgcgccat cattgcttta cgcgccccgt cggcatcacc agcaaaaatg
     9721 gcgtcaatga cagcctgatg ttgttcggtc agttgtgaaa aaaccggtgg caccagatac
     9781 atccgctgac ggctatgctt cactgaggat tgcaggacat cgaagaaacc gcgcatggtt
     9841 tgcagcagca cgatgttatg tgaggcttcg gcaatcgcca gatgaaaacg aacgtccgct
     9901 tgtgaggcga tatccgggtc ttcacttagc gttgcttcaa agcaaagctg aatcttttct
     9961 ttgtcgccag gtgtggcgcg cattgccgca tgccatgcgg tgctggcttc aatggcgtag
    10021 cgggcttcca gaatatcgaa actgtaatcc ggatcatcgg ccatcagcgt ttttagcggc
    10081 tggacgatgt tttgctccga ccatgtgtca tgacgccagc gaataaacgt cccgccgccg
    10141 cgtcgactga gcagcacgcc ttcgctcacc agttttgcca gcgcctcgcg tagtgaatta
    10201 cgtgatacgc cgagttgcat cgccagttgg cgctcagcgg gcaacttcat gcccgcttcc
    10261 aggttttttt catcaatcag cgcccgcaca cgatcggcaa cctcgtctga caggcgtctg
    10321 ggtaaaacaa tcattaagga atcatccacg ttaagacata agcctgaagc gtggtgatca
    10381 cgcccaccat acaggtgaag atcaggctgt gtttgacagt aaagcggaac aaatcagact
    10441 ctttgcccac caggcctacc gccgcacagg cgatagcgat agattgcggg gagatcatct
    10501 taccggtgac gccaccggtg gtattggcgg caaccagcaa cagatcagag acgccaattt
    10561 gttgtgctgc ggtggcttgc agcgcggcga acagggcgtt agatgaggta tccgacccgg
    10621 tcaggaatac ccccagccag ccgaggaacg gcgagaagaa ggtgaatgca tgaccggtgt
    10681 gcgccagtgc cagcgccagt gttgatgaca gtccggaata gttcgaaata aaggcgaatg
    10741 ccagcaccat accgatggag tatatgggca gagccagttc tttcagcgtg ctgccgaagg
    10801 tgctgatagc gtcagacggt ttcatcttca gccagacaat cgagagcagt gcagcaaaca
    10861 gaatggcggt gccggtggca gagaaccagt caaacttaaa cacggcggca taggctgtag
    10921 cctcgctgac cactggcggc atacgggcaa ccagtttatc gaggtacggc accggaatat
    10981 tgatcaccca ctcatacagc gcgccacccg atgcgaacag ggctttaaac ggcgggatac
    11041 tccacagtgt tacggtagct gtcaggaaca ggaacggtgt ccaggcacgt aacacctgac
    11101 ccgcagtgta accggtgtgg gccagcgtca tatcaacctg tgacgccccc aaatcaccaa
    11161 aacggaatac acgcactggc tgccagcgtt tgaggaacag cgtcaggcag agcagtgata
    11221 ccagcgaaga gataatgtcc ggcagctccg gcccaatgaa gttagagcta aggtactgag
    11281 cgatggcaaa cgagccgccc gcaaccacga ccgcaggcca cgtctctttg ataccgcgcc
    11341 agccgtccat aatcgccatg atccagaaca gcacgataat ggtcataaac ggtagctgcc
    11401 gccccaccat ctgaccaatc tcaaagctgt cgatacctgt tacctgtccg gcaaccagga
    11461 ttggaatgcc catcgcacca aatgccactg gcgcggtgtt aacaatcagg cacagcccgg
    11521 cggcgtacag cggtttaaaa cccaggccga ccagcaatgc ggcggtaatt gctaccggtg
    11581 cgccaaagcc tgcggctcct tcaaggaacg cgccgaaaca gaaaccgacg atcagcattt
    11641 gcagacgctg gtcaggggtt atcgaaagaa tagacgagcg aatgatgtca aactgcccgg
    11701 ttttcaccga gatcttatag acgaacaccg ctgcaataat gatccacgcg atgggccaca
    11761 acccgtagaa gaaaccataa accaccgagg ccagcgcgtt agcgaccggc attttataga
    11821 acagcaaagc cacggcaagg ggcgattgcc accgtccacg aggcggcgac gtatcctttc
    11881 agtttgagct taatcagcgc aaagaagaaa aacaggatgg gaagcgatgc tatcagactg
    11941 gaaagccaga tattcccggc gggatcgtag ttttgttgcc agagattcat tgcaggtctc
    12001 ctggagtcca cgcacatcgt gtaatgagtc tgttgctcat ctccttgtca cacgttgtgt
    12061 aaaagtggca ctgccaattg tgttgtgtag ggataatgac aatgaatggt taactaaatg
    12121 ttatgtattg gcaatattga tgtgagtttt aattggggaa ttgtgaacca gaggtcagat
    12181 aagcgaagaa ttggtagggc caattcttgt ggggggagat gtctggtaaa gaaaagggca
    12241 atcctcatca gagaattgcc cttagattgt tgacccggag cgccttatct tgcctgatgc
    12301 gcgtaacgca tcaggcaatt tggcgtttgc ccttagtcac actaacttaa tggatcatta
    12361 ccactgaata ccggcaccga gtgcggcgga gtattcaccc tggctattgg tactaccttg
    12421 taatttgtag acccaacgac cattggcgct caccatcgat acacctaaag caactgccga
    12481 ttcaccgttg taagtaccgc caccaataga agccatgctg gcacccggtg tataagcctg
    12541 cggcagaccg gtcattgcca ttgcagaagc gataccaccg ctcaacttgc tttcagtttt
    12601 agacagtttg ttatccatct caaccattcg ctgatcggtg tattgcttcg tttcctgcac
    12661 gctttgcttc aactgcgcgt aattcaccgc gtcgttgttg ttgacgccag cggagacgtt
    12721 gctgatacga gtcgtaccgc cgttgccgcc accgagggtg atattgctat agtcgataga
    12781 accatcagct ttggtgtcgt aacgcacacc gccagcttcg gaagacttca actgtgccac
    12841 gttaacagca tcggtgtcgt ttttacctgc ggctacgttg gtgatacgac gttgattagt
    12901 agatgagcct acagagatcg tattttcttc ggttgccaca gaccctgtac ccagtgcgac
    12961 gctgttatcc gcggcagcaa tggagccaga accaatcgcg acgctatctt taccctgcgc
    13021 gctggcatct acaccatcgg tattggtctt gaagtacttg gtgctaccgg tggtgacgat
    13081 atcgccaata ccgttttcga tattggtgac gcgtgaatca agattcgtta cccgattatc
    13141 caggttagta atgctctggg tattctggga aacctgcgca tccgttactt tcaactgacc
    13201 cacgttgacc gcatcggtat cagccgaacc tgctgcgacg ttagtgatct gacgttgacc
    13261 atccgcacta ccgactgaga attcaccgac agagttctgc ggtgcgtcca tgttgtaggc
    13321 ggtataattg gtttgagcgc cacgagtggt cgtagaacca ttacctatcg caatactgtt
    13381 gacatgaatg acttgcgcat tgctaccaat ggcaatgccg ttaagtgcat tcgcgtccac
    13441 gtaggcacca taaccgatac caataccttt atcgccattc acgatggttt tcgcacccat
    13501 tgccagcgag tcagttccca ctgccagtga atcttcttcc gttgaattag cgtggaagta
    13561 tttagtcggc gtggttgcga ccgcaccaat cgcattctgc aattgacgaa ccgtaacggc
    13621 gtcatgggct tcggaaccat cggctacgtt gatgatttga cgatatttac cgtcatcacc
    13681 gatagacaat gcaccgagca gttcgccatc cgtggtgtcg taaccaataa caacgccatt
    13741 ttccgttatg ctggtgtcac gggagccttt ggcaatcact cggctggaaa cagagctgct
    13801 acccagggcg atacccgtat caacgtcgct gtagctgccc tgaccaatag ctacgctgct
    13861 atcgcctttg gcgacagagt tataacctat agctgtagcg ccaacaccct gtgcgctggc
    13921 gtcgttgaac gctaagccgt cgtcgttagt acgcacatag ttaatacccg caccgttttc
    13981 ttggatatag gttgcgtcgg tgttaccagc gagctgattg ataatttggg tgttctgctc
    14041 aatcatcata ttcgtcgcat tcaactgaga accgttcact gcatcggtac tgtcctcact
    14101 aatactgcca ttagcgacat tagtgatgat gctggcttta ccgtcatggc tggcattgta
    14161 ggctccagca ccgccattgg cagtctcatc ccacagcagg gcgttatcat caagcgcatc
    14221 cagggcgtca ccgacgttgt tgtactcggc gttcgcaatg gtgtaagtcg gtgcggtgat
    14281 ggtgccgtca gcgttgactt ctgcgtcacc acccagcgca tcagcgatgt acttattggt
    14341 ggtatagagt tgtgagccgt taatcgcgtc gctgctggca gcagagattg caccgttagc
    14401 gacgttagtg attacactgg cagttttatc ttttccgtga gcggcgctaa atgcaccatt
    14461 ttcaccggcg tccgcatccc agagcagagc gtcgtcaaga gtggtatcga tagcattcag
    14521 ggcatcaccg acattatcgt aatcagcatt agcaatggtg tacgtcggcg cagtgatggt
    14581 gccgtctgca ttgacttcgg caccaccccc cagcgcatca acaacataac tgctcacgcc
    14641 gtggagttgt gagccgttta ctgcgtcaga actggagtct gaaatttcac catctgcgac
    14701 atcagtgatc acgcttgcgt caccattagt accgtgtttg gcactgaatt tacctgcggt
    14761 ggcatcccaa agcagagcat cgccgagaga cgtgctaaat gaggagttaa tcgcagccag
    14821 tgcatcgccg acgttataat aatttgtttc accgattttg taggttggac cggtaaacac
    14881 accgtcagta ttcacagaag cattgccgcc gaagtaggtg gcgatattgc tgcttaagtc
    14941 ataaagctgg ctaccgttaa tggcatcgga actggttgca gtgactgtgc cgtccaggat
    15001 attggtgatt ttgctggcgg tattgttgcc atgagctgca gtgaagacac cattgtcctt
    15061 atcccatttc agcgcatcct caccaagatt agtcaccgtc tcagtcagat tagagatgtt
    15121 ggtggtgtta gtggcgatat tggtggtatt cgtcgccacg gcatcgttgg tggttttcag
    15181 ctgagagccg ttaacggcat cggtgctgcc agtcgtcagg tcgccatctt taacgttggt
    15241 gattttgctg gtggtctcgg tgccatgagc tgccgtgaat acaccattgt ccttatccca
    15301 tttcagcgca tcctcaccaa gattagtcac cgtctcagtc aggttagaga tgttggtggt
    15361 gttagtggca atattggaag tgttattggc gatattggtg gtattcgtcg ccacagcatc
    15421 gttggtggtt ttcagctgag agccgttaac agcatcagtg ctgtcagccg tcaggtcggc
    15481 atctttaacg ttggtgattt tgctggtggt atcctggccg tgagctgcac tgaatgcctt
    15541 tttagtttcg ttccagagca gggcatcagc ctgaaggtca ccaacggaat ccgtcaggtt
    15601 ggtgatattg gtggtattgg tggcaatatt tgccgtattc gtggcaatgt tgctggtatt
    15661 agtagcgata ttggcggtgt tggcttcgac atcgtcattg gtagctttca gttgggaacc
    15721 gttaaccgca tccttactgg aggctgaaat cgtgccatcc gcaacatcgg tgattacgct
    15781 ggcagttggg ctactagtac catgagcagc gctgtatttg cctttggttt ggtcccattg
    15841 cagggtgttt tcatcaagta cagcgagcgc agcccctacg ttatttttgc taccattttt
    15901 taaattgtag gttggtgctg taacagtacc gtcatcaaca tctactgcag cccctcctcc
    15961 aagccttttt gctaccgagt cgctaatggc ataaagctgt gaaccattaa tggcatcgta
    16021 actgtcagac ttgatcgcgc catttttcac attaacgatc ttgcgtttta atgaactatt
    16081 gccgacagag acggtattgt ctaaatccgc aatggaatct gcgcccagcg cgacagagtt
    16141 aataccgttg gcactgctat tactaccaag cgcaagactc ttatcaccag ttgcattact
    16201 ggctgtgcct aaggcaatgg tgtttaagcc tctcgactgg ctaccattac ccagagcgat
    16261 ggcatccacg ccatcagctt tgttgccctg gcctatggcg attgcattat ctgccagagc
    16321 ctggctgcca tttcctaatg cgatagtatt gactttatcg gcaaggcttc cgaaccccat
    16381 tgccatgcta ttagtccctt ttgccttcgc attattgcca atagcagttg cattttcagc
    16441 ggcttcggtc tcagcaccta tcgcaatagc gttcgctgcg ctggcaagtg attgtctgcc
    16501 aaatgcgagg ctgtcagtac cgctagctac actgctacgc cccagggcaa ttgcgttttc
    16561 ttcagatgcg acgctacttg ctcccagcgc cattgaatac gcttttgacg cattggcggt
    16621 gtcgccaaga gcaatactca taatctcagt agctttagtg gcatttccca gggcaatggc
    16681 gtttgctcct tccgctttgg aagagtcccc catagcaata gacaatttac ccaatgcttt
    16741 tgaataacgg ccgagggcca ttgagtattg gcccaatgaa tacgaagagg cacccagtgc
    16801 tatacttcta tcgccttctg atattgcact aaccccaaag gccattgatg caccgccaat
    16861 cgcatgggtt tttgacccga tggcagagct atattggcct tcagctatag cgtcataacc
    16921 cacagcagta ctggaaccac tggtgttcat aaaagtattt gctttcgccc ctttgcctat
    16981 agcaacccag ccgtcgccag ctgatccact accgtaatca acaccctgac cgttgtcatt
    17041 cccggcattc gccaatgccc caaacgacga caacattcca cccgcaacca gcgcagaaat
    17101 taacagctta ctgcgcccag atttcttgcc acggcttttt gccgtttcgc tggtaacagt
    17161 ataattccct gtcgcagggt tccagataac tttaaatatt ttgttcatta atgatgctcg
    17221 ctattccttg ttataagcgt actaactgca tttatttttt gcttcttttc tgccgatttt
    17281 cagcagaggg aagattgtcg aactgaatat tttccatgtt gatggctaac ttcagatatc
    17341 ccaggttatt gggcgataca cccttcaggg tcaaattaat gtcttgccat tgacctggta
    17401 ataatttcaa tgtggatttc attgatgtgt tgaaaagtaa ttgttgccac cgggcaaact
    17461 ctttggcatc gacaggtttt tcaccacccc atgtcgcatt aaaaccaata tttgagatag
    17521 gtgtacttaa taagttgacc agtgaaatat gaataacact gccattctct gtggcgtcaa
    17581 tggataacac tcgacccgct aaagccaccg aaccattgtt caggatgaaa tacttcgttt
    17641 ttgctgggtt aaactcggta aaaataaaag tgttttgttg atctttaacg gatttaatta
    17701 cgtgagttaa agtctttgta ctgcttatta tgccagtttg ttgtttttga atagtttcca
    17761 gttgactctt tagctgtgct tgttgttttt caagtagatc aattttattg ttatgttctt
    17821 cttgttgttt caataaacta ctttctggct tgccttcttc aatgtagtca catcccgtta
    17881 atgcgcagag aactgaaaca gtgacagcac tgaaaaaaat cttcttcaag ttcatgtaaa
    17941 aatccttatt tcctccgacc agcgatcaat gccggtagag ttgtccattg tattattggt
    18001 gatctatgtt gctttacatt aatgacagtg atgtgtgtcg attaaatatt gttttaagca
    18061 ttcat
//
LOCUS       DAZGVQ010000104        18040 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000104 DAZGVQ010000000
VERSION     DAZGVQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.105"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            153..2393
                     /gene="fepA"
                     /locus_tag="ACHLAG_003409"
     CDS             153..2393
                     /gene="fepA"
                     /locus_tag="ACHLAG_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFP0656574.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            2433..2531
                     /locus_tag="ACHLAG_003410"
                     /pseudo
     CDS             2433..2531
                     /locus_tag="ACHLAG_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2568..3188
                     /gene="entD"
                     /locus_tag="ACHLAG_003411"
     CDS             2568..3188
                     /gene="entD"
                     /locus_tag="ACHLAG_003411"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFP0656575.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(3310..3462)
                     /gene="hokE"
                     /locus_tag="ACHLAG_003412"
     CDS             complement(3310..3462)
                     /gene="hokE"
                     /locus_tag="ACHLAG_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFP0656576.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            3431..3700
                     /locus_tag="ACHLAG_003413"
     CDS             3431..3700
                     /locus_tag="ACHLAG_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656577.1"
                     /translation="MTATRAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            3904..5022
                     /gene="ybdK"
                     /locus_tag="ACHLAG_003414"
     CDS             3904..5022
                     /gene="ybdK"
                     /locus_tag="ACHLAG_003414"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFP0656578.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFTSSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            5088..5336
                     /gene="ybdJ"
                     /locus_tag="ACHLAG_003415"
     CDS             5088..5336
                     /gene="ybdJ"
                     /locus_tag="ACHLAG_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFP0656579.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            5401..5769
                     /gene="ybdF"
                     /locus_tag="ACHLAG_003416"
     CDS             5401..5769
                     /gene="ybdF"
                     /locus_tag="ACHLAG_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP0656580.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            5863..6516
                     /gene="nfsB"
                     /locus_tag="ACHLAG_003417"
     CDS             5863..6516
                     /gene="nfsB"
                     /locus_tag="ACHLAG_003417"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFP0656581.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            6624..7871
                     /gene="ybdG"
                     /locus_tag="ACHLAG_003418"
     CDS             6624..7871
                     /gene="ybdG"
                     /locus_tag="ACHLAG_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFP0656582.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(7939..9315)
                     /gene="pheP"
                     /locus_tag="ACHLAG_003419"
     CDS             complement(7939..9315)
                     /gene="pheP"
                     /locus_tag="ACHLAG_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFP0656583.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(9417..12560)
                     /gene="cusA"
                     /locus_tag="ACHLAG_003420"
     CDS             complement(9417..12560)
                     /gene="cusA"
                     /locus_tag="ACHLAG_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFP0656584.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(12572..13795)
                     /gene="cusB"
                     /locus_tag="ACHLAG_003421"
     CDS             complement(12572..13795)
                     /gene="cusB"
                     /locus_tag="ACHLAG_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFP0656585.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(13811..14143)
                     /gene="cusF"
                     /locus_tag="ACHLAG_003422"
     CDS             complement(13811..14143)
                     /gene="cusF"
                     /locus_tag="ACHLAG_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFP0656586.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGEV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(14167..15549)
                     /gene="cusC"
                     /locus_tag="ACHLAG_003423"
     CDS             complement(14167..15549)
                     /gene="cusC"
                     /locus_tag="ACHLAG_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFP0656587.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            15706..16389
                     /gene="cusR"
                     /locus_tag="ACHLAG_003424"
     CDS             15706..16389
                     /gene="cusR"
                     /locus_tag="ACHLAG_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFP0656588.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLMKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            16379..17827
                     /gene="cusS"
                     /locus_tag="ACHLAG_003425"
     CDS             16379..17827
                     /gene="cusS"
                     /locus_tag="ACHLAG_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFP0656589.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFV
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYNSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         4389 a         4809 c         4557 g         4285 t
ORIGIN      
        1 taatggcgcg ccgtgggaag cgcggacatt tattaaccaa ctgcactgcg tgtctttcag
       61 gatcaaaggt tttcgcggta gcgggatgcg tcgtgttgag gacgaccatg cccgacagtt
      121 gcaattcgtg gcaaaaatgc aggaataaaa caatgaacaa gaagattcat tccctggcct
      181 tgttggtcaa tctggggatt tatggggtag cgcaggcaca agagccgacc gatactcctg
      241 tttcacatga cgataccatt gtcgttaccg ccgccgagca gaacttacag gcgcctggcg
      301 tttcgaccat caccgcagat gaaatccgca aaaaccccgt tgcccgcgat gtgtcggaga
      361 tcatccgtac catgccaggc gttaacctga ccggtaactc caccagtggt cagcgtggga
      421 ataaccgaca gattgatatt cgcggtatgg gtccggaaaa cacgctgatt ttgattgacg
      481 gcaagccggt aagcagccgt aactcggtgc gtcagggctg gcgtggcgag cgcgataccc
      541 gtggtgatac ctcctgggtg ccacctgaaa tgattgaacg tattgaagtt ctgcgtggtc
      601 cggcagctgc gcgttatggc aacggcgcgg cgggcggcgt ggttaacatc attaccaaaa
      661 aaggcagcgg tgagtggcac ggctcctggg acgcttattt caatgcacca gaacataaag
      721 aggaaggtgc cactaaacgc accaacttca gcctgaccgg tccgctgggc gacgaattca
      781 gcttccgctt gtatggcaac ctcgacaaaa cccaggctga cgcgtgggat atcaaccagg
      841 gccatcagtc cgcgcgtgcc ggaacgtatg ccacgacgtt accagccggg cgcgaagggg
      901 tgatcaataa agatattaac ggcgtggtgc gctgggactt cgcaccattg caatcgctgg
      961 aactggaagc gggttacagc cgccagggta acctgtatgc gggcgatacc cagaacacca
     1021 actctgacgc ttacactcgc tcgaaatatg gcgatgaaac caaccgcctg tatcgccaga
     1081 actacgcgct gacctggaac ggtggctggg ataacggcgt gaccaccagc aactgggtgc
     1141 agtatgaaca cacccgtaac tcgcgtattc cggaaggtct ggcgggcggt accgaaggga
     1201 aatttaacga aaaagcgaca caggatttcg tcgatatcga tctcgatgac gtgatgctgc
     1261 acagcgaagt taacctgccg attgatttcc tcgttaacca gacgctgacg ctgggtacgg
     1321 agtggaatca gcaacggatg aaggacttga gctccaatac ccaggcgctg accggggcga
     1381 acaccggcgg cgctattgat ggtgtgagtg ccaccgaccg tagcccgtat tcaaaagcag
     1441 aaattttctc gctgtttgcc gaaaacaata tggagctgac tgacagcacc atcgtaacgc
     1501 cggggctgcg tttcgatcat cacagtattg tcggcaataa ctggagcccg gcgctgaaca
     1561 tatcgcaagg tttaggcgat gacttcacgc tcaaaatggg cattgcccgt gcttataaag
     1621 caccgagcct gtaccagact aacccgaact acattctcta cagtaaaggt cagggctgct
     1681 atgccagcgc gggcggctgc tatctgcaag gtaacgatga cctgaaagca gaaaccagca
     1741 tcaacaaaga gattggtctg gagttcaaac gcgacggctg gctggcgggc gtcacctggt
     1801 tccgtaacga ttatcgcaat aagattgaag cgggctatgt ggctgtaggg caaaacgcag
     1861 tcggcaccga tctctatcag tgggataacg tgccgaaagc agtggttgaa ggtctggaag
     1921 gatcgttaaa cgtaccagtt agcgaaacgg tgatgtggac caataacatc acttatatgc
     1981 tgaagagtga aaacaaaacc acgggcgacc gtttgtcgat catcccggag tacacgttga
     2041 actcaacgct gagctggcag gcgcgggaag atttgtcgat gcaaacgacc ttcacctggt
     2101 acggtaagca gcagccgaaa aaatacaact acaaaggtca gccagcggtt ggcccggaaa
     2161 ccaaagaaat cagtccttac agcattgtgg gtctgagcgc gacctgggat gtgacgaaga
     2221 atgtcagtct gaccggcggc gtggacaacc tgttcgacaa acgtttgtgg cgtgcgggta
     2281 atgcccagac cacgggcgat ctggcagggg ccaactatat cgcgggtgcc ggggcgtata
     2341 cctataacga gccgggacgt acgtggtaca tgagcgtaaa cactcacttc tgatgctaac
     2401 gtcagattgt tgacaaagtg cgcgtcgttc atgccggatg cggcgtgaac gccttatccg
     2461 gcctacaaaa atcttgccaa ttcaatatat tgcaggacca tgtaggcctg ataagcgtag
     2521 cgcatcaggc agtttagcgt ttgtcatcag tctctaatat ggtcgacatg aaaactacgc
     2581 atacctccct cccctttgcc ggacatacgc tgcattttgt tgagttcgat ccggcgaatt
     2641 tttgtgagca ggatttactc tggctgccgc attacgcgca actgcaacac gctggacgga
     2701 agcgcaaagc cgaacattta gccgggcgga tcgctgcaat ttatgcgctg cgggaatatg
     2761 gctataaatg tgtgcccgca atcggcgagc tacgccaacc ggtgtggcct gcggaggtat
     2821 acggcagtat tagccactgc ggggctacgg cattggccgt ggtttcccgt caaccgattg
     2881 gcgttgatat cgaagaaatt ttttctgcgc aaacagcgac cgaattgaca gacaacatta
     2941 ttacaccagc agaacacgag cgactcgcag actgcggttt agccttttct ctggcgctga
     3001 cactggcatt ttccgccaaa gagagcgcat ttaaggcaag tgagatccaa actgatgcag
     3061 gttttctgga ctatcagata attagctgga ataaacagca ggtcatcatt catcgtgaga
     3121 atgagatgtt tgctgtgcac tggcagataa aagaaaaaat agtcataact ctgtgccaac
     3181 acgattaatt gacaacatct ggtacgattc gcccgcagcc atcactgacc gctggcgaaa
     3241 gtgtaaagca ggtgccttac cactctgacc tgacaaccgg atatgcgggg attgctcccc
     3301 gcacaacggc tacttcttcg gttcgtaagc gagtacagcc ttaaactcaa tattacgttc
     3361 ctttacggtg aactcacaca gcgagtctcc gaccagaagc gtaaatccca gtaccgtcaa
     3421 acacagcact atgactgcca caagggcata tttcgtcagc atgtttatat tgcctccatg
     3481 cgaagaaggg actaccatcc aaattgttca ggtttgggta ggtagcccca gggttgatag
     3541 aaatatcgtc ctggggcttc atctttccag acctcagagt aaacctgaaa ccggaaagct
     3601 tcaggcaccc gcagttatct tacccttgct tagcaaaata gctgtttatt tatacagcaa
     3661 gtcctgtttc ggacttctca ctccccattt gatcaactga agctgaaccc gtttttttct
     3721 gcatgcctca atcgtcaaaa ttcttcgcca ccagccagcc gtgccataac ttgtcacatt
     3781 gcaaaagtag ccaaattgct tcagtcacca tcaccaaaaa aagaaacatt ccagaacttc
     3841 cgcatctcgc ccagccgctc cgacttctat actgaataga aaacgccaac ttaagagaaa
     3901 cctatgccat tacccgattt tcatgtttct gaacctttta ccctcggtat tgaactggaa
     3961 atgcaggtgg ttaatccgcc gggctatgac ttaagccagg actcttcaat gctgattgac
     4021 gcggttaaaa ataagatcac ggccggagag gtaaagcacg atatcaccga aagtatgctg
     4081 gagctggcga cggatgtttg ccgtgatatc aaccaggctg ccgggcaatt ttcagcgatg
     4141 cagaaagtcg tattgcaggc agccgcagac catcatctgg aaatttgcgg cggtggcaca
     4201 cacccgtttc agaaatggca gcgacaggag gtatgcgata acgagcgcta tcaacgaacg
     4261 ctggaaaact ttggttatct cattcaacag gcgaccgttt ttggtcagca tgtccatgtt
     4321 ggctgcgcca gtggcgatga cgccatttat ttgctgcacg gcctgtcacg gtttgtgccg
     4381 cacttcatcg ccctttctgc cgcgtcgccg tatatgcagg gaacggatac gcgttttacc
     4441 tcctcacggc cgaatatttt ttccgccttt cctgataatg gcccgatgcc gtgggtcagt
     4501 aactggcaac aatttgaagc cctgtttcgc tgtctgagtt acaccacgat gatcgacagc
     4561 attaaagatc tgcactggga tattcgcccc agtcctcatt ttggcacggt ggaggttcgg
     4621 gtgatggata ccccgttaac ccttagccac gcggtaaata tggcgggatt aattcaggct
     4681 accgcccact ggttactgac agaacgcccg ttcaaacatc aggagaaaga ttacctgctg
     4741 tataaattca accgtttcca ggcctgtcgc tatgggctgg aaggcgtcat taccgatccg
     4801 cacactggcg atcgtcgacc gctaacggaa gacaccttgc gattgctgga aaaaatcgcc
     4861 ccttccgcac ataaaattgg tgcatcgagc gcgattgagg ccctgcatcg ccaggtcgtc
     4921 agcggtctga atgaagcgca gctgatgcgc gatttcgtcg ccgatggtgg ctcgctgatt
     4981 gggctggtga aaaagcattg tgagatctgg gccggtgact aagtagggaa ttgccttaca
     5041 gaagaatatc cctgacaatg gtgttttaac ttacatttaa caatgttatg aaacaccctt
     5101 tagaaacctt gactaccgca gcaggcattt tgctgatggc tttcctctct tgcctgctgc
     5161 tgcccgcccc cgcactgggg ctgacgctgg cacaaaaact ggtgaccatg ttccatctga
     5221 tggatcttag ccagctttac actttattgt tttgtctgtg gtttttagtg ctgggcgcta
     5281 ttgagtattt tgttctgcgc tttatctggc gacgctggtt ctcgctggcg gattaaacgt
     5341 cgattatcgc cgtacttcgc ggcatacttt gcttattctc cttcgcctaa aggaacgttt
     5401 atggataagc aatcactgca cgaaacggcg aaacgcctgg cccttgagtt accctttgtc
     5461 gagctttgct ggccttttgg cccggagttc gatgttttta aaattggcgg caaaattttt
     5521 atgctgtcgt cggagctgcg cggcgtcccc tttatcaatc tgaagtccga tccacaaaaa
     5581 tccctgttaa atcagcagat atacccgagc attaagccag gatatcacat gaataaaaag
     5641 cactggattt cggtgtatcc cggcgaggaa atctccgaag cgttacttcg cgatctgatc
     5701 aacgattcgt ggaatctggt ggttgatggt ctggctaaac gcgatcaaaa aagagtgcgt
     5761 ccaggctaaa gcggaaatct atagcgcatt tttctcgctt accatttctc gttgaacctt
     5821 gtaatctgct ggcacgcaaa attactttca catggagtct ttatggatat catttctgtc
     5881 gccttaaagc gtcattccac taaggcattt gatgccagta aaaaacttac cccggaacag
     5941 gccgagcaga tcaaaactct cctgcaatac agcccatcca gcaccaactc ccagccgtgg
     6001 cattttattg ttgccagcac ggaagaaggt aaagcgcgtg ttgccaaatc cgctgccggt
     6061 aattatgtgt tcaacgaacg caaaatactt gatgcctcgc acgtcgtggt gttctgtgca
     6121 aaaaccgcga tggacgatgc ctggctgaag ctggttgttg accaggaaga tgctgatggc
     6181 cgctttgcca cgccggaagc gaaagccgcg aacgataaag gtcgcaagtt cttcgctgat
     6241 atgcaccgta aagatctgca tgatgatgca gagtggatgg caaaacaggt ttatctcaac
     6301 gtcggtaact tcctgctcgg cgtggcggcg ctgggtctgg acgcggtacc catcgaaggt
     6361 tttgacgccg ccatcctcga tgcagaattt ggtctgaaag agaaaggcta caccagtctg
     6421 gtggttgttc cggtaggtca tcacagcgtt gaagatttta acgctacgct gccgaaatca
     6481 cgtctgccgc aaaacatcac cttaaccgaa gtgtaattct ctcttgccgg gcatctgccc
     6541 ggctatttcc cctcagattc tcctgatttg cataaccctg tttcagccgt catcataggc
     6601 tgctgttgta taaaggagac gttatgcagg atttaatatc ccaggttgaa gatttagcgg
     6661 gtattgagat cgatcacacc acctcgatgg tgatgatttt cggtattatt tttctgaccg
     6721 ccgtcgtggt gcatattatt ttgcattggg tggtactgcg gaccttcgaa aaacgtgcca
     6781 tcgccagttc acggctttgg ttgcaaatca ttacccagaa taaactcttc caccgtttag
     6841 cttttaccct gcaggggatt atcgtcaata ttcaggcggt attctggctg caaaaaggca
     6901 ccgaagcggc agatattctg actacctgcg cgcagttgtg gatcatgatg tatgcgctgc
     6961 tttcagtctt ctcgttgctg gatgttattt tgaatctggc gcagaaattc ccggcggcat
     7021 ctcagttacc gctgaaaggg atatttcagg ggattaaact gatcggcgca atcctggtcg
     7081 gcattttgat gatctcgctg ctgattggtc agtcgccagc gattctgatc agcggtcttg
     7141 gtgcaatggc tgccgtgctg atgttggttt ttaaagatcc gattcttggt ctggtggcag
     7201 gtattcagct ttccgcgaac gatatgctga aactgggcga ctggctggag atgccgaaat
     7261 acggcgcgga tggcgcggtg atcgatattg ggttaaccac cgtcaaagtg cgtaactggg
     7321 acaatacgat taccactatt cccacctggt ctctggtttc tgactccttt aaaaactgga
     7381 gcgggatgtc agcatctggc gggcgacgta ttaagcgcag tatcagtatt gatgtcacca
     7441 gtattcgttt tcttgatgaa gacgaaatgc aacgtctgaa taaagcgcac ttgttaaaac
     7501 cttatttaac cagccgccat caggaaatta atgagtggaa tcgccagcaa ggttctacgg
     7561 agtcgatatt aaacctgcgc cgaatgacca atattggaac ctttcgtgcc tatctgaacg
     7621 aatatctgcg taaccatccg cggattcgta aagatatgac tttaatggta cgccaactgg
     7681 ctccaggtga taacggttta ccgctcgaga tctatgcgtt taccaacacc gtggtgtggc
     7741 tggaatatga aagcattcag gctgatatat tcgatcacat atttgcgatt gtcgaagagt
     7801 ttggtctgcg acttcatcag tcgccaaccg gcaatgatat tcgctctctg gcgggtgcat
     7861 ttaagcagta attaaaaaaa ccgctctcat cgaatggatg agagcggaca tcgttttgtc
     7921 ggatgtagcg tgaatgcctt atttccgacg cagcgtttta aatgccacaa acaggaatac
     7981 aatccacacc ggcagcagga tcgctgacaa gcgcatatca tccatcgtgc acatcagcag
     8041 caaaatcatg ccgaggaagg caatgcaaag atagttgccg aacggataga gcagcgcctt
     8101 aaactgtgtt tcacgcccct gacgtcgcat cgctgcacga aaacgcagat gcgccaggca
     8161 gatcataatc cagttcaaca gcagcgttgc taccaccagc gccatcagca gaccaaacgc
     8221 tttttgcggc agcagatagt tgattaatac caccagcgaa gtgatcgctc cggaaagcat
     8281 cagcgaatta atcggcacac cgcgacggct gacgcgagtc aaaaacttcg gcgcattacc
     8341 ctgcacagaa aggccaaaca gcatgcggct gttagagtaa accccgctgt tatacactga
     8401 cagcgatgct accagaatga cgaagttcag cgcagaagct accacgttgc tgtcgagatt
     8461 atggaaaatc atcacaaacg ggctactgtt ggatttcact tccacccacg gatagagcgc
     8521 cagtaaaacc accagtgagc cgatgtaaaa cagcaggatg cgatacacca cctgatttac
     8581 cgcttttgga atgctttttt ccggatcgcg cgcttcagcg gcagtaatcc caatcagctc
     8641 cagaccgccg aaggagaaca taattaccgc cagcgacaaa atcagcccat tccagccggt
     8701 ggcaaagaaa ccaccgtagc gccagaggtt gtcgatactg gctttctcgc cgccgtgacc
     8761 agaaaacagc agccacaggc caaagccgat cataccgatg atcgccagca ctttaatcaa
     8821 cgcaaaccag aactcggttt cgccatataa gcgcacgttc accaggttaa cggcgttgat
     8881 gataataaag aaggcggcag cccaaatcca cgttggaaca tccgggaacc agtactgcat
     8941 atagatgccc gcagcggtca gctctgccat ccccaccagc acgaacatca cccagtagtt
     9001 ccagccagag aggaagcccg cgaacggtcc ccagtattta taggcaaagt gggcaaacga
     9061 accggatacc ggctcttcga ccaccatctc gccgagctgg cgcataatca ggaaagcgat
     9121 gatcccggcg acgccgtagc ccagcaatac agccggaccc gccatctgaa tcgccgggcc
     9181 aatgccaaga aacagaccag taccaattgc gccacccagc gcaatcagtt gaatatgacg
     9241 gttatgtaat ccgcgatgaa gcgtcggctc ttgattcgac gcagtatctt ccgatacggt
     9301 tgacgcgttt ttcacgcctt tcccctgtgt gtcttttttg ttgaggggca ccttttaaca
     9361 tttagtgccc atcgtcgcaa gacacaatcc acacggttaa acggggtatc ctgcttttat
     9421 ttccgtaccc gatgtcggtg cagccacatc agcttatacg ccgccgggat aataaacagc
     9481 gacagcaaag gtgcggtgat catgccgcca atcatcggtg cggcaatccg gctcatcacc
     9541 tctgaccctg ctcctgttcc ccacagaatc ggcagcagac cagcgataat caccgccacc
     9601 gtcatcgctt tcgggcgcac gcgcagtacc gcgccgtgat acaacgcctc atccagcttc
     9661 tgctcgctga atgtttgcgg attattcaac gacggctcgg cctctatggc gtgacgtaaa
     9721 tacatcagca tcaccacgcc aaattcggcg gcgaccccgg cgagggcgat aaagccagtg
     9781 cccgtcgcca cggaaagatg aaagcccatc caccacagca accagatgcc gcccaccagc
     9841 gcaaacggta cgctgctgat aatcagcaac gcttcgccca cccgacggaa cgccagatac
     9901 aacagcacga agataatcat caacgtcatc ggcaccatga gtttaagctt atggttggcg
     9961 cgctccagta gctcgaactg cccggagaat gccacgctgg tgcccggttt taactgcact
    10021 ttttcagcta tcgctttttg caaatcgtga accaccgaca ccatgtcacg atcgcgggca
    10081 tcgatataaa tccagctcgt cgggcgcgca ttctcggttt tcagcattga cggtccggta
    10141 gagactttaa tgtcggccac gtctgccagg gtgatttgct gcttcatcgg cgtcagaatc
    10201 ggcagctggc gcagtgcctg cggactatcg cgccagcttt gcggataacg cagattaatt
    10261 ggataacggg caattccttc caccgtttcg ccaaccatcg ccccgcccac agcagaagtc
    10321 acaaacaact gcacatccgc caccgtcata ccgtaacgcg cggctttttc acggttaatc
    10381 tcaacgttga tatagcgccc accttccagc cgctcggcaa gagcagaagc tacgcctggc
    10441 accgttcgcg ccacttcttc aatttgctca gccatcgcgt cgatatccgc cagcacagtg
    10501 ccggaaactt taatgccgat ggggctttta atgccggttg agagcatatc gatacggtta
    10561 cgaattggcg gcacccacag attcgccagc cccggcagcc gcacggtgtt atccagttcc
    10621 tcaatgattt tgtccatcgt catgcctggc cgccactgct cctgcggctt aagctggatg
    10681 gtcgtttcta ccatctccag cggagcagaa tcggtggcgg tttccgcttt cccggttttg
    10741 ccaaataccc gcgccacttc aggtacgctc ataattagct tgtcggtttt ttgcagcata
    10801 ctcgccgcct ctgctgcgga aatccccggc agcgtcgatg gcatatacaa caagtcgcct
    10861 tcattgatct gcggtaaaaa ttccccgcca actttattga gcggccagag aaccgtcagc
    10921 accgaaagcg ccgccaccag cagcgtggtt ttcggccagt gcagtacttt cagcaacagc
    10981 ggatgataaa cacgaatcaa aaagcgattg agcgggttac tgctttccgg cggaattttg
    11041 ccacggatcc agtagcccat caggatcggg atcactacga tcgccagcag cgccgcaccc
    11101 gccatcgcat acgttttggt gaacgccaac gggccaaaca gacgcccttc ctgcccttcc
    11161 agggtgaaga tcgggataaa cgacaacgtg ataatcagca gactgataaa tagcgccggc
    11221 cccacttcaa cagacgcatc ggtgatcacc tgccagcgcg ttttattatc cagcgtggcg
    11281 tcaggatgct ggtgctgcca ctcttccagc cgtttatgtg cattttcgat catgacgata
    11341 gcggcatcga ccatcgcccc gacggcaatc gcaatgccac ccaacgacat aatattggca
    11401 ttcagtccct ggaagtgcat gacaataaaa gcaatacaca accccagcgg caacgaaata
    11461 atcgccacca gcgccgagcg cacatgccag agaaacagcg cacagactac cgccaccaca
    11521 ataaactctt ccagcaactt gccgctgagg ttgtcgatgg cgcggtcaat gagctggctg
    11581 cgatcgtatg ttgtaactat ctccacgcct tccggcagac tacttttcag cgtttccagt
    11641 ttgtccttca cggcggcgat cacttctcgg gcgtttttgc cggatcgcag gatcaccacc
    11701 ccgcccgcca cttcgccttc gccgtttagt tcggcaatgc cccggcgcat ctccgggcca
    11761 atctggacct tcgcaacatc gcgcagataa acgggcacgc cattttcact ggcttttaaa
    11821 acgatgtgat taaagtcgtc gagcgtttgc agatagccgc tggcgcgcac catatattcc
    11881 gcttccgcca gttcgatcga cgaaccgccc gcttcctggt ttgaagcatc cagcgcgctt
    11941 tttacttcgg cgagactgat gccatactgc gccaggcgct ggggatcgat aaccacctga
    12001 tactctttca ccacaccgcc caccgacgcc acttccgcaa cgtcagggat ggttttcagc
    12061 tcatatttga gaaaccagtc ctgtaatgag cgtaaatcgg ccagatcgtg cttaccgctg
    12121 cgatccacca gtgcatattc atagatccag ccaacacccg tggcatctgg tcccagctcg
    12181 gcgctgactc ccgcaggcag cttaccctgt acctggttga ggtactccag cacccgcgaa
    12241 cgcgcccagt acggatcggt gccatcttcg aaaatgacat acacataaga gtcgccaaac
    12301 tgtgagaaac cgcgcacagt cttcgcgcca ggcaccgaca acatggtggt ggttagcgga
    12361 taagtcacct gattttcaac gatttgtggt gcctgaccgg gatagctggt tttaataata
    12421 acctgcacat cggagagatc cggcagcgca tccactggcg tattaatgat ggtccaggtg
    12481 ccccagatgc tcagaaacaa cgcgcccatc agcaccaaaa aacggttcgc caccgagcga
    12541 cgaataatcc attcaatcat tggttattcc ctcaatgcgc atgggtagca ctttcagagc
    12601 gcatccgctc cagtgcgcca gaaatattgg cttcggaatc aatcaggaac aggccgctgg
    12661 aaaccacctt ttcaccttcc gccagaccag agcgtaatgc ggtgacgcct tgcgatgcct
    12721 ggaaaacagc aacgcgtttc ggtacaaagc gcccgtcggc atcaacggta atcacccgct
    12781 gttcgctgcc ggtatcaatc agcgcttgtg acggaatgag cagcatcggt tcgctggcgg
    12841 tgttgagttg caaccaggcg ttcatgcccg gttttagcgc ctcgtcggcg ttgtcgactt
    12901 ccagacgcag ctgcagcgtg cgggtcgcgg catccacgcc aggtagcagc gtccatttgc
    12961 ggatggtgag tgttttatcc ggtcgcgccg gaacggtgag ggtaaactgc gaggcatctt
    13021 tcaccagcca ggcgatagac tccgggatcg cagcagtgac ccacaccggg tccataccct
    13081 gaattttcgc taccacgtta tctttggcga tattcattcc cgcgcgcaga tcaaacgcgg
    13141 tgatcacgcc atcaatgggc gctttgagcg taaagcgagt ctggattttt tgcgtggcga
    13201 tcaggcggcg aatatccgcc tccggcattc ccgccagtcg cagccgctca agaatgcctt
    13261 cagtctgggt cgccgtaccg ccggtttcgc gcagcagtaa atactcactc tgcgcttcca
    13321 cccagtcagg aatggtcagg tcgagaagcg gtgtgccctt ttgtacttta tcgcccacgg
    13381 taagcggata caccttgtcg ataaacccgg cagcgcgagc ctgcacaatg gcatactgat
    13441 actcgttgta actgacattc gccgggaaac tctgggcaaa agtcagcggc ccgcgcgtga
    13501 cggtagccgt tttcaccccc agattctgcg tctgagtcgg gtcaatgcgc acaccagacg
    13561 cagaactctc ttcatcggca tatttcggca ccagatccat atccataaac ggcgatttac
    13621 ctggtttatc gaaccgcgta ttggggtaca tcgggtcgta ccagaataag attttacgtt
    13681 ctgcggtcga cgttttttct gcgggtggtt cagcctttgc aaaccaggta aaacctgccg
    13741 cagaaataat accgcccgcg atcatgctgc cgataataag cgcgattttt ttcatctcat
    13801 taaacctggg ttactggctg actttaatat cctgtaataa agaaaggttg ccctgctgga
    13861 caaaattaaa cgccactttg tcgccggttt taatttcact cattttcgtc tgcggggtga
    13921 tggtaaagcg catggtcatc tccggccagt tcacagcagc aatcggatcg tgatggatgg
    13981 tgattttttt gctttccaga tcaataccct ttacctcgcc agtggcacta ataacctgtg
    14041 gttgtgcttc gctcatggtt tcatgatgat gttcgttagc ctgggcatta aagccaataa
    14101 cggtaaacag actgaacatt gcgacttgca gtgctttttt catttattct ctcctggagt
    14161 taaaagttat tgctgccaac cgccaccaag tgcggtatat aaagaaattt cgttaacctg
    14221 acgggcataa ttcaggtcaa gtaaagtttg tcgggttgca aataacgaac gttcggcatc
    14281 cagcacttcc agataactta ctgcgccgtg ctgatataat gcccgcgccc gttgcaaagt
    14341 aatttgcagc gatgccagat aacgctgctg ggcgctaatt tgatcgttca ggctttgacg
    14401 taatgccagc gcatctgcca cttctttaaa ggcgttctgg attttctgtt cataattcac
    14461 taccgactgc tgctggcgaa tttcggcgat atccagattg gcctggttgc gtccggcatt
    14521 aaaaatgggg atctcaattt taggaataaa attccacatc ccgctgctgg cgttaaataa
    14581 tgacgacaga tcgctgctgg cggttgatat tccgctggtc aggcttatag atgggaaaaa
    14641 tgccgcgcgt gcggccccaa tattggcatt agccgccatt aacgcgtgtt cagcttccat
    14701 aatatccggg cgctgcaata agatttgcga cgacaagccc gccggtaatt taacgctttg
    14761 caggctgtcg ctgtttactg tctgcgcttg cggcagcttg ccgtagcttc ccagcaacag
    14821 ttgcaatgca ttattcgcct gcgccagttc cccctgacgt ttagcgatgt cgctgcgggt
    14881 actttctatc accccgcgag cctgttccag cgccagaaca ttactgctac ctgtcaacag
    14941 ttgtttttcg acaaacgcat atgactgctg ataattacgc agcgtttctt cagctatttg
    15001 caattgcgca tacgccagtt gctgattgaa atagctttgc gcgacattag aaaccagcag
    15061 aatatgcacc gcgcgctgag cttcctcagt ggctaaataa ttttggcgct cggcttcgct
    15121 catgttcttt aagcgaccga aaaaatcgag gtcaaagctg gcgttaaggc cggtcgaaaa
    15181 ctcccgtgtc gtggcggtat tccctttaag attaccgctc cagctgccgc tgccctcgcc
    15241 attgagctgt gggtagcggt cggcatcggt cagacgatat tgcgcccgcg cttcctgcac
    15301 tttcagcgtc gccatgcgca aatcccggtt attcaccagc gcctcgctaa tcagcgtctt
    15361 tacctgatta tcaacaaaaa aggtgcgcca gcccgcgttc tggtagttat ctgctgcgtt
    15421 aaccaagccg ttctggctga gtgagaactg ctgcggcacg ggcattgccg gacgctgata
    15481 atccggtgcc agtgaacaac cggttagcgc aagggccaca caaaatggca gaagtttaca
    15541 aggagacata ggctcataat ttctggtgat tttataccgc caactttact cgccaggctc
    15601 tgattttccg gtgacaggaa aatgacaaaa ttgtcatttt gccaataagc gattgccatc
    15661 tgatcccgct actctagaat tgcccgggca acatgcggag gaaaaatgaa actgttgatt
    15721 gtcgaagatg aaaagaaaac cggagaatac ttgaccaaag ggttaaccga agccggtttt
    15781 gtggtcgatt tggccgacaa cgggctgaat ggctaccatc tggcgatgac cggtgattat
    15841 gatctgataa tcctcgatat tatgctgccg gacgtgaacg gctgggatat cgtgcgcatg
    15901 ttacgctccg ccaataaagg gatgccgatt ctgttgctta ccgcgcttgg caccattgaa
    15961 catcgcgtca aggggctgga gttgggggca gatgactacc tgatgaaacc attcgctttt
    16021 gctgaactgc tggcgcgggt gcgcactctc ctgcggcgcg gggcggcggt aattatcgaa
    16081 agtcagtttc aggttgccga cctgatggtc gatctcgtca gccgcaaagt cacccgcagc
    16141 ggcacgcgca tcactctgac cagtaaagag tttactctgc tggagttttt tctccgccat
    16201 cagggcgaag tgctgccccg ctcgcttatc gcctcgcagg tgtgggacat gaatttcgac
    16261 agcgacacta acgccattga tgtggcggtg aagcggctac gcggcaaaat cgacaacgac
    16321 tttgagccga agctgattca gaccgtgcgc ggcgtgggct atatgcttga ggtgccggat
    16381 ggtcagtaag ccatttcagc gcccgttttc gctggcaacc cgtctgacct tttttatcag
    16441 cctggccacc atcgcggcgt ttttcgcctt tgcatggatc atgatccact cagtgaaagt
    16501 gcattttgtc gagcaggata ttaatgattt aaaagaaatt agcgccaccc ttgaacgggt
    16561 acttaatcat cctgacgaga cgcaagcccg acgcttaatg acgctggaag atatcgtcag
    16621 tggttattcc aacgtgttga tttccctggc agatagccac ggcaaaacgg tgtataactc
    16681 ccccggtgcg ccggatatcc gcgagtttac gcgtgacgcc atacccgata aagacgctca
    16741 gggtggcgag gtgtatctcc tttccggccc gacgattatg atgccaggcc acggtcacgg
    16801 gcatatggaa cacagcaact ggcggatgat taacttaccg gttggcccgc tggttgacgg
    16861 caaaccgatt tatacgctct acatcgcgct ttcgatcgat tttcatcttc attacataaa
    16921 tgatttgatg aataaactta ttatgaccgc atcggtaatc agcatcctga tcgtctttat
    16981 cgtgctgttg gcggtacata aaggtcacgc gccgatccgc agcgtcagcc gtcaaatcca
    17041 gaatatcacc tcgaaagatc tcgacgttcg ccttgacccg cagaccgtgc ccattgagct
    17101 ggaacagctg gtactgtcgt tcaaccatat gatcgagcgt attgaggatg tctttacccg
    17161 ccagtccaat ttctcagcgg atatcgctca cgaaattcgc acgccgatta ccaatctcat
    17221 cacgcaaacg gaaatcgccc tcagccagtc tcgcagccag aaggagctgg aagatgtgct
    17281 ctactctaat ctcgaagagc tgacgcgaat ggcgaaaatg gtcagcgata tgctgtttct
    17341 cgctcaggcc gataacaacc agctaatccc cgaaaagaaa atgctcaacc tggcggatga
    17401 agtcggcaaa gtgttcgatt ttttcgaggc gttagcggaa gatcgcggcg tggagttgcg
    17461 atttgttggc gacgaatgtc aggttgcggg cgatccgctg atgctgcgtc gggcgttaag
    17521 caacctgctt tctaatgccc tgcgttatac gccacccagt gaggctattg tagtgcgctg
    17581 ccagacggtc aatcatcagg tgcaagtttc cgtcgaaaac cccggtacgc ccattgcgcc
    17641 cgagcactta ccgcgattgt ttgaccgttt ctatcgcgtt gacccttccc gccagcgaaa
    17701 aggtgaaggt agcggtattg ggctggcgat agtgaaatcg attgttgtcg cgcataaagg
    17761 cacggttgcg gtaacgtcag atgcgcgggg gacaaggttt gtgattatgc tgccggagag
    17821 agagtgatta ttaagttcaa attcagggcg tggtgcatca cctcctggta ttgttgatca
    17881 acaaattaga gaaaaactta ttaaacatat gaaatatcga aggcttttat ttttcaaagt
    17941 ttcacgagat gtttgtatat gaaacttagt taaatctgtg tttcttccaa ttgtagatca
    18001 caatgccctt tctaaatttt catcgttata aacaagccat
//
LOCUS       DAZGVQ010000105        18010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000105 DAZGVQ010000000
VERSION     DAZGVQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.106"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(72..2942)
                     /locus_tag="ACHLAG_003426"
     CDS             complement(72..2942)
                     /locus_tag="ACHLAG_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFP0656590.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGQAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(2939..3718)
                     /gene="ygfM"
                     /locus_tag="ACHLAG_003427"
     CDS             complement(2939..3718)
                     /gene="ygfM"
                     /locus_tag="ACHLAG_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFP0656591.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(3769..5097)
                     /gene="ssnA"
                     /locus_tag="ACHLAG_003428"
     CDS             complement(3769..5097)
                     /gene="ssnA"
                     /locus_tag="ACHLAG_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFP0656592.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(5100..8198)
                     /gene="ygfK"
                     /locus_tag="ACHLAG_003429"
     CDS             complement(5100..8198)
                     /gene="ygfK"
                     /locus_tag="ACHLAG_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFP0656593.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(8520..9098)
                     /gene="mocA"
                     /locus_tag="ACHLAG_003430"
     CDS             complement(8520..9098)
                     /gene="mocA"
                     /locus_tag="ACHLAG_003430"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFP0656594.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            9201..9971
                     /gene="yqeC"
                     /locus_tag="ACHLAG_003431"
     CDS             9201..9971
                     /gene="yqeC"
                     /locus_tag="ACHLAG_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFP0656595.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            10019..11644
                     /gene="yqeB"
                     /locus_tag="ACHLAG_003432"
     CDS             10019..11644
                     /gene="yqeB"
                     /locus_tag="ACHLAG_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFP0656596.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFSSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(11685..12617)
                     /gene="arcC"
                     /locus_tag="ACHLAG_003433"
     CDS             complement(11685..12617)
                     /gene="arcC"
                     /locus_tag="ACHLAG_003433"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP0656597.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(12665..14050)
                     /gene="hyuA"
                     /locus_tag="ACHLAG_003434"
     CDS             complement(12665..14050)
                     /gene="hyuA"
                     /locus_tag="ACHLAG_003434"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFP0656598.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(14103..15314)
                     /locus_tag="ACHLAG_003435"
     CDS             complement(14103..15314)
                     /locus_tag="ACHLAG_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFP0656599.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(15372..16568)
                     /gene="dpaL"
                     /locus_tag="ACHLAG_003436"
     CDS             complement(15372..16568)
                     /gene="dpaL"
                     /locus_tag="ACHLAG_003436"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFP0656600.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(16626..17813)
                     /gene="ygeW"
                     /locus_tag="ACHLAG_003437"
     CDS             complement(16626..17813)
                     /gene="ygeW"
                     /locus_tag="ACHLAG_003437"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFP0656601.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
BASE COUNT         4167 a         4530 c         4723 g         4590 t
ORIGIN      
        1 ctcattttcg cagtcactat ttagtcatcg tccaaacaga gaaacaggcc gccgaagcgg
       61 ccatttatta tttatatttt ttccagcgca gtgagtattt tctccggtgt gaaatgccat
      121 tcgcgtaacc agatgccgca tgcatcgtga attgcggtag caatcgccgg agctgcgcca
      181 tttacaccga tttccgagat cgatttcgcc ccgaacgggc cgactttatc gtcgctcggc
      241 accagcacag cgcggaaatc acgcggaatg tcaccaattt tcggtgcgcc gtaactgcgt
      301 aaatcacgcg ttaacgggtg accttcggcg tcataaatga tctcttcgct catactgtgg
      361 ccgatagctc gcagggtagc accgtagatt tgtcccaatg ctaattccgg gttgaccggt
      421 gtaccgcagt ccagcaaggc gtagaattta tccaggcgga tttcacccgt acgcgtgttg
      481 acggcaactt cagcgaagtt tgcgccatac gggaaggcga aatcaggcgt gatataactg
      541 ccagttccca ccagtgaacc aaagccggtg ccggtttcgc ctttatgagc aatatccccg
      601 aagctgactt cgcctttctt gccgcgcacg acgcccggcg ttgccagttg aacatctgcc
      661 actgcctgac ccaacatttg cgcgccgtgg aacagaattt tctcccgcag attttccgct
      721 gccaaacgcg ccgcgttacc tgagaagcaa gtaccggacg aggcatatgc gcctttatca
      781 aacaacgcat gatcggtatc accggagata acatgcacgt cctgcggtgg gcagtgcagc
      841 acttctgctg ccagtttcgt cactacagta tccagaccag taccaatatc cgcaccgcca
      901 gaatgaacga taaaggtgcc gtccgattcc agtttgatca tgcagttagc ctgatcgata
      961 tccgggatac ccgatttctg catgataatg gcgacgccgc gaccgatgtg ccagtcacca
     1021 ttttgcggtt tcggtgacga ccattggatc atctcgcgac cctgacgcag gatctcttct
     1081 agtgcacagc tggcggcgga aggaacggag gtcggcgctt taccttcacc gattgcaccg
     1141 agaattttca gctcttgccc ttcgtgtacc cggttacgtt cgataatttc cagttggtcg
     1201 atctgtaact gttcagccag ttccgctaat gccatggtga tagcgaagtt acctttcggt
     1261 gcgccataac cctgataagc accatttggg caaatgttgc tgtagtaggt ggtgacctgg
     1321 aaatcgacgt tatcgcacgg atataacggc agcgacagcg ccggtccgtt acacggtacg
     1381 gtgagtgagt ggttgccgta agggccagtg ttggcgcgga aatccatctt cactgccgtc
     1441 aggcgaccat cttttttcgc tcccagtttg acggtgactt tcgcgacgtg acgagaggtg
     1501 ttagcaataa actcttcttc acgggtgtag cggaacagta ccggacgccc ggtcacgcag
     1561 gttgcccagg cgcacacttc ttccagcagg atgtcctgtt tggaaccaaa accgccgcca
     1621 actcgctctt taatgacatg aactttatgc tgtttcatgc ccacgaggcg cgcgacctgg
     1681 cggcgtaagt gccatggtac ctgggtggag gcgtggataa ccagacgatc gccgtccatc
     1741 cgggtaaagc agatatgtgt ttcagtcggg cactgctgtg cctgcgttga gttataggtt
     1801 cgctcaatga tcacatcggc atcggcaaag cctttgtcca tatcgccaat atgaccatga
     1861 atactggcgg cgatattttt gcgagggcga gaaccgatcg ggaagttgat gatcatatgc
     1921 tcgccgcgct gggctgcatg gctgttatcg tcttccagag tatctggcgc accagcaaca
     1981 tacaccaccg gttcatcgtg cacgacaggc gcatcttccg ccattgcttc gtcgatcgac
     2041 attaccggct taagcacttc atattcaacg tcgatgagct tcaatgcttc gagcgcaatt
     2101 tcttcacttt ctgcgacgac cgcagcaaca cgatcgccga cgtgacgcat tttcttgccg
     2161 aacatacggc ggtcaagcgg tgacggttcc ggtgcgctct gaccacccgg ggtatagtag
     2221 atatccgggc aattcaggtg agtaataacg tgaacgacgc ccggtaaggc ttcagctttg
     2281 ctgacatcca gatgagtaat cagtgcgtga gcgtgtgggc tacgtaacat tttaatgacg
     2341 caggcgtcag ccgttacgcg gtcttcaaca tagcagggtt tcgcctgcac cattttcgcg
     2401 gcatcagttt taggataatg cttgccaatg acttcaaggt cgtcacggaa agtcggagcg
     2461 atatcaatgg tggcctgcgg attattttta cgtgcaaccg ccagttcaat gacctgatag
     2521 tattgttgcc agcctgcatc gcggctgaac aaaccagaaa gcgcgtcgtc gatctcttcg
     2581 cgggtaggtg cggcgatgcg atcgagaaga tcggtgataa tcagagctgc agctggatcg
     2641 ttataaccag actgcaccac gccaacatca accattgcct gttgaaccag acttaactcg
     2701 ttccatttgc ccaaagattc tgcggtacga atatctgcct tctctaactg tgcggcaata
     2761 agcaaggacg cgttaacgat attaccgtta aagattattg cgtcagaacc ggcaaacccg
     2821 aagccatcat cactgttgcg tacagagtgc attcccatgt taaacaacag cttttgcacg
     2881 ttttcgcctg gattaacggt tagctcctga ggcgcgccat ttaaagtaaa gtggatgatc
     2941 atacggcttc ctccccagcc tgttggcagt cggcatacag gtcggctacc agaactcccg
     3001 tgatatagcg tttataggcc acgctgcccc gcaaatcttc ctgcgggaaa atggcgttgg
     3061 cgacagcctg ttcaagtgca ttgccttcca gattttgttt ttcgacatca tgcagacgca
     3121 gtgctttact ggcgacgcca tccagcgcaa ttcgcatacc gtcgtggtct gtcattgcaa
     3181 cggctgccgt cacgacggtt aaacctgcct gagaacggct aattttgcgg gtcgcacagg
     3241 tgcgatacgg atctttaatg ataatttcgg ttagcaggcg atcgcatggg caggccaggt
     3301 agtcctcgat tgacagcgtt tcgccgttgc caaaaaccag ttcagcatcc agtgccaaca
     3361 ggacgggaag cagcaccgac tcttcctggc gggcggcgat ttcaccacca atggtcgact
     3421 gattacgaac atggcgtgag taaacaaaac cgagggcttc acgcagcgct gcaggaataa
     3481 atcgcgcatc acgcagtggc tgcaagcgag acattgcgcc aatccgcagt gcaccgttat
     3541 cccagtcaac ccagtccagt tccagatcct gtaaggaaat ggcaatcttt ttatcggtac
     3601 gggttggtgt agcattgagt ttgctgcccc cggcgaacca gacggcttca tcctggtagc
     3661 ggcgcttcag ttccagcgcc tgttcgacag agtcgggcct gaaaaattgt tcaatcatct
     3721 tctttcctta tttaaccctc ttccctgcgg gaagagggca tctgtcattt atgccagcgc
     3781 atccatccga cgccacatac tggcagcggc ttttctggct tgtgcataaa tggaatcgca
     3841 atcgaagtta aactgacggt cttcatagac catcacacca ttgaccatca cgctgtgaac
     3901 gctgcctgag cccataccga aagcgatatg cccggcgata ttgtctgcca gcagcggcgt
     3961 cggtgagttg taatcgcaaa tggttaaatc agctttgtaa ccggcttcca gaagtccaaa
     4021 tttcgcgcca aagttgcggc tcatcagttc gttaccgtta gtcagggctt tggcaaaact
     4081 gtcaggccac agcggaccac ccgcatcgcg atgtttaaag aaggcaaatt tcatctcttc
     4141 aaacatgtcc gaaccaatgc cgtccgttcc caacgccaga ttgcggatgt cgctaaggtg
     4201 atggttgtag ccgacatggt tgttcatgtt tgaacgggcg ttatgcacca ggaacgcatc
     4261 gcgctgattg agtagggcga tgtcatcttt cgacaagtac agcccatgag cgaccagcgt
     4321 tttgctatcg atgagatcga attgcgccag tcgtgccagc aggtctttgc cgtaccagtg
     4381 gtgactgtag gaaacgtcgt aaaggtcttc cgcagcgtga atatgcaaac cacggcctgt
     4441 ggctttcacg gcttcacgta gcatctccag accggcatct ggcacggtaa acggcgcgtg
     4501 agcaccgata tgtgcttcca ccagatacgg ctcggttgct gctttcttcg cctcatcaat
     4561 ctgacgggcg aagcggatgt tttcttctac accttcctgc aactctttga tgccgttgtt
     4621 acggtcagta gtttcaaaac aggtcatcgc gcgcaggcca acttttaaaa atgcgtcgcg
     4681 caatgtggag agcgacccgc cgatatacgc cggagaggca tggtgatcga taaccgatgt
     4741 acatccgctc ttaatcgctt ccagggaaca aatcagcccg ctgtaataga gcgactcttc
     4801 atcaagggcg cgatcgagcc gccaccagag atttttcagc gttgagatga aatccgggca
     4861 gggggcgatg tttgccataa ttccgcggga aagccccgag taaaaatggt tgtgcgagca
     4921 gacaattccc ggcatcacaa tccgaccatg catctctttg tagctggcat cggggtagcg
     4981 ttgcgtcagg gcatcgccga tagcgacaat cacatcattt tcgatagcga tatcaacgcc
     5041 ttcctgcact ttcgccgggt gtaactgcac tgcggtgaca ttcttcagaa tcaacatgat
     5101 tacacctcca cgcggcccag cagataatga tgatgctggt ggacatggct gatgatgcgg
     5161 cacatatcgt tcagctccgg tggtacgttg ttgaactgac cttcgctgtc gatgtttaac
     5221 acccagcttt ggttattcag tcgtactcgt acccggcaat cttccacaag gaagcctggg
     5281 ttgctgctgt tatcaaagtc ttgcgccagg ctgaaaacgg tgattttgtc tttgtatggt
     5341 ttaccgttcc acggacagaa ctgagcgcag ttgccgcatt cgttacagta agcgtcgagg
     5401 tgcagcgtct ggaatctatt ctggaagcct gggaccgcaa tggagacgtt ggcgcggttc
     5461 gggcagacat ccacacactt gctgcaaacg tagttacatt cgaggcagcg agcggcttcc
     5521 tgggcgacaa acgcgtcacg atcgtcactg ttcaccagag tgatagagat atcgccttta
     5581 cgttgataga tttccgccgg attgacgttg ttccagtatt tatcgttctg gtgggaacgg
     5641 atattttccc gactaaggat ggcatcggtc gcccgacgcg cggttccgac agcagcgaca
     5701 atggaggatg gtccgcgctg tacgtcgccg atcataaaga cgtcagtcag acgagtttcg
     5761 ccattgtggt cgacgtctgg ccagccgttt ttgtccagcg gcacgcccat cgcattcagg
     5821 gcttcagtat cctgctgttc accaatggcg gtgatcaggc tgtctacatg cagtgttact
     5881 gtttcgttgg tttcaaccgg acggcgacgg cctttctcat cgggttcgcc aagcgacata
     5941 acgcgcaagg ttaaggtgcc atcagcatcg aaacgttccg gattattcag gaaacggaac
     6001 tccacgccgt cgtgcaacgc ttcttcatac tcttcgcgcc atgcgggcat ctcttgcagt
     6061 gaacgacggt aaacgatcgt tgctttttct acgcctggaa cgcgtaacgc cgcacgagcg
     6121 cagtccattg cggtgttacc cgccccgaca acgaccacat gtttgcccag cttgagcgct
     6181 gtacccttgt tgtattcacg caggaagggg agtgatttcc aaacattttg gttgtcaccc
     6241 gccagtttca caccgctatt tttatcagtg ccggtggcaa tcagaacata gtggaagccc
     6301 tgatttttta actgttcaac ggtcaaatcg ggtgagcagc catactcaaa tttcacgccg
     6361 tgatcggcaa caaaatcgat atcgtgctga attaactcag ccggaattcg gaactgagga
     6421 atgatatttt tcaccacgcc gcccgcattg gcttcgcgtt caaacagcgt aaccggatgg
     6481 cccgctctgg caaggaagta acctgctgcc agacccgccg gacctgcacc aatcacggca
     6541 accggatggc gtgaaccaga accggctggt ttgtgccagc gttgcttata ttcatcccaa
     6601 cctttttcca gcgcgacttt tttcagttcg cggatattca gcgcactgtc gtaatccagg
     6661 cgggtacagt tgtattggca ctggtgatcg caaatatgac cggtaatggc gggcagagcg
     6721 ttgcgttggt agatgagttc cagcgcgtcg gcatagcggt gttcgccaag cagacggatg
     6781 tattccggaa tatcttgctt gatagcgcag gcagtaacac agggggcaac gtagcaatcg
     6841 gtcagcggca ggtcttctgc cacttcaata cgctcttctg gcttccagtg tttctgggtg
     6901 tattccatgg ttaacgcatc tgctgccagt ctgttcagtc gttcgacgtc aacatggtca
     6961 agtccccagg cgtcggagcc ttccagctcg cgcatgcagg cacttaagcg caaatagccg
     7021 ccaggtttca gcaggtcggt tgccatagta atagggcgaa tacctgtatc aaaaatatcg
     7081 cggatagtca gctgactggc accaccggaa taagaaatgg gcagtttgcc gtcaaaggcg
     7141 cgagagagaa ctgctgcaac attgatggag agcgggaaca gcgcacggcc tgacatatac
     7201 atctcttcac caggcagtgc gcctttattg ttgatggtgc cgagagtgtt agtcagtttt
     7261 acgccaaagc cgagtgattt ttcttttgcc agtgccatca ggcgttccag catttccagt
     7321 gcttgcgtca gcttgaggtc gtgatcaaat gactcttctt ttaagcctat gtaaccgaaa
     7381 ccgcagacat cgaggatctc acgaacacgc gcgtatccca gtaaggtcgg gttaagtttc
     7441 acaaaggtgt tgagcccttt ttcttccagc atgtagcggc aaatggcttc gatttcatgc
     7501 ggaggacagc cgtgcatggt ggagagggtg acgccctgca ccatactggt ggggatgcga
     7561 gcgggtaagg cttgcaagct ttcgcgtttt tcctgcaatc cgtggcgggc taaaaatgcg
     7621 tcatcctgga gtaatttatt cagcgtatcg cgatattgcg cgaatttcgg atggtcagat
     7681 gcgtccatca tattgtcgat gaactgttgc attggcggtt gcttaatacc ttcgaggttg
     7741 taaccgacgc tcatattaaa gatgaacgat ttaccggaat cagaaggctg gaacatcgct
     7801 tcgagaaggt gcagggcaaa ccaggctttg aggtattcat cccaggcttt aagcagggta
     7861 aattcggtag accattcggt gttaaagcac tcgtcttcgg catcgataca gggcttttcc
     7921 agctccaggc ggtcaagaat ttggacggtt tttagttcga tgaagcgtcc gccagtcagc
     7981 caggaagtga caatattttg cgcgagctgc gtgtgcgggc cagcggcagg gccgacggga
     8041 gtggcacagg tttcaccgaa gacgctaaca gttttacctt ttacaggtga gtaaaactgt
     8101 tgctcgggaa taccaaagat tgagcgttgt tgttggtatt catcaaatat gcgcgtcaaa
     8161 agttcctcaa acggaatggg acgcataata tcccccataa ccctctcctt agcttataca
     8221 gatatcattg agtgaccggt gttgagaaat ccggtggttt agggggatag agcaacaatc
     8281 gcaccactct tgccaggttt ggcgggaaat ataaaaaaat gtgaggcggc tcgaaactta
     8341 aaggcttttt gtgaatgaac tcacaggtgc caaatgagaa ttaaaatcac tcctgaaaat
     8401 tattattgtc agcccattta tcactttttc tcttttctca aaatcagtga ttccttatca
     8461 ttatgataaa gagtgctgga gagattaaat atttgaacgc aatcgtttaa ctacattttc
     8521 taaattttag tatacctttt ttgcgctgta ataaagtcgt ccggagtatc aatatctaaa
     8581 attatttctg cattttctat ctcgacggaa taatgagctc ccataaggag tgcctgacgc
     8641 atattggtga cattgggtcg ttggattgcc tgcatcaagc atggttttga cactaaaatc
     8701 ggatggccgg ggatgccatt atggagtggc agtattgcgc catcatttcg taacgaccag
     8761 atttttctaa aaatatcgat ggtgagggtt ggcatgtcac cgtgggtgag aaaacaatgt
     8821 tctgtttgca ctgcgggtac tgcggccttt actgatgtta gtaaaccctg cgcataatct
     8881 gggttgtgga taatagtgat attgctctgg ttcgcataac gttcgtgcag ttcattacca
     8941 cgatagccgg tgactaaaat aattcggcta caaaactgca acgcattttt gatacttgta
     9001 tcaagaattg tcccctgttg ccagggtaac atcattttcc attgccccat tcttgatgat
     9061 aatccggcag cggtaattat acagtcgatg gctgacatag tggttccatt atttataggg
     9121 gctaataacg cctgataatt gcgttacttc ctgatggatt aagttgggga taatttatcc
     9181 cagagaggtc ataaagactc gtgaaaagta tagttgaccc atcggcatta gtcattgatt
     9241 taggtgcgca gaaacgtccc actgtgattt ctgttgttgg tgctgggggg aaaaccagct
     9301 tgcttttttg gctggcagaa ctgctccagg ctagcggcag gcgtgtgtta atcacgacga
     9361 cgacacatat gtttatgcca acgtctcact ggcccgtggt tttctgtcgt gatcccgcca
     9421 tgcttcctca tgcgtctctt acatccccca tttcattttg ttttcacagc tggaaagcga
     9481 accagggaaa agtgcaggga tttacgccag aagcgattga tgcactggtg caacgaccag
     9541 agtgtgacgt aattctcatt gaagcagatg gctcgcgtgg aatgccgtta aaagcgcctg
     9601 atgagcacga accttgcata cccaaaagca gttgctgcgt gattgctgtg atgggaggac
     9661 atactttggg cgcgaaagtg agcacagaaa atgtccatcg ctggtcgcag tttgctgata
     9721 ttaccgggtt aacacctgat gcaaccttgc aactgagcga tctcgttgcg ctggttcgcc
     9781 accctcaggg ggcgtttaaa aacgtaccgc aaggttgtcg gcgagtctgg ttcattaacc
     9841 gtttttctca atgtgagaat gcgattgcac aaagcgagct ccttcaaccg ctgcaacaac
     9901 acgacgtaga ggcaatctgg ctgggcgata tacaagaaca tcctgcaatc gcgcgcagat
     9961 ttgtgaatta gcggcccggg tattccgggt tactgaaagg atatccattg aggttggtat
    10021 gaatattttc acagaggctg caaaactcga agagcaaaat tgtccgtttg cgatggcgca
    10081 aattgttgat agccgaggct cgactccccg ccattctgca caaatgttag tgcgcgccga
    10141 tggttctatc gtcggtacaa ttggtggcgg aatggttgag cggaaggtga ttgaagagtc
    10201 acttcaggca ttgcaggaac gtaagccgcg attattccat ggacgtatgg ctcgtaacgg
    10261 tgcggatgct gtcgggtcag attgtggagg tgcaatgtca gtatttatca gcgtccatgg
    10321 tatgcgtcca cgtctggtgt tgattggcgc ggggcatgtc aaccgggcga tagcccagag
    10381 tgcggcgcta ttaggatttg atattgccgt tgccgatatt tatcgcgaaa gcctcaatcc
    10441 tgaactattc cctccatcaa ccacgcttct ccatgctgag tcgtttggtg cagcagtgga
    10501 agcactggat attcgccctg ataattttgt cctgattgcc acgaataatc aggatcgtga
    10561 agccctcgac aaactcattg aacagcccat tgcatggttg gggttgctgg caagtcgccg
    10621 caaggttcag cttttcctgc gtcaattgcg tgagaaaggc gtggctgaag aacatattgc
    10681 ccgtttacat gcgcccgttg gttacaacat aggtgcggaa acgccgcagg agatcgccat
    10741 cagcgtgttg gcagaaatat tacaggtgaa aaataacgcg ccgggtgggc tgatgatgaa
    10801 accttctcat ccttccggac accagctggt ggtgattcgc ggtgcgggag atatcgccag
    10861 tggtgtggcg ctacgtctgt atcatgcggg ttttaaagtg atcatgctgg aagtggaaaa
    10921 accgacagtg attcgttgta cggtggcgtt tgcccaggcc gtgttcgatg gcgaaatgac
    10981 ggtcgaaggc gtcactgctc gcctggcaac cagctctgcg gaagcgatga aacttaccga
    11041 acgcggattc atccctgtga tggtagatcc cgcctgttca ttgcttgatg aactgaaacc
    11101 gctttgcgtg gtggacgcta tcctggcgaa acagaatttg ggaacgcggg cagatatggc
    11161 accagtaaca atcgcgcttg ggccgggctt tactgcaggg aaggattgtc atgcggtaat
    11221 tgaaacaaat cgcgggcact ggctcggtca ggtgatttac tctggttgtg cgcaggagaa
    11281 taccggtgtt cctggcaata ttatggggca taccacccga cgggtgatcc gtgctcctgc
    11341 tgcaggcatt atgcgatcca acgtgaaatt aggcgatctg gtgaaagagg gcgatgtgat
    11401 tgcctggatt ggtgagcatg aaattaaagc accgttgacg gggatggtgc gtggcttgtt
    11461 gaacgacggc ttggcagtgg ttggtggttt taaaattggt gatatcgatc ctcgtggtga
    11521 aaccgctgat ttctccagcg tttctgataa agcccgggcg attggcggcg gcgtacttga
    11581 ggcgttaatg atgttgatgc atcagggcgt gaaagcgaca aaagaagtgc tggaagtggc
    11641 ttaaatgaaa atggccggat ggtattaccc acccggcctt aaaattactg acaaatcacc
    11701 gttccggttt tgccttcaat cccttctttc gctttgctca atacggtaat cagtgcttca
    11761 cgacctgcgc gagagcgagc aaacgaggcg gcagcttcca ctttaggcag catagaacct
    11821 ttcgcaaaat gaccttcttc gataaagcgt tcagcatcac ttaatgacag gcgatcgagc
    11881 cactgttcat tctctttacc gaagttaatg gccacttttt ctaccgcagt cagaatgatc
    11941 agcatatcgg catcaatcat ttctgctaaa cgggcgctgg cccagtcttt atcgataacc
    12001 gcgctggcac cacgcagatg gttaccttca cgaataacag gaataccacc accgccaacg
    12061 gtgatcacca cctggcctgc atctaccaga gctttaaccg tttctttttc aataatatca
    12121 accggttttg gcgaggcaac aacacggcga tagccgcgac cagcatcttc tttcagagtg
    12181 taaccctgtt ttgtcagttg ctctgcttct tgctcggtaa agaacgaacc gatcggcttg
    12241 gtcgggttga ggaatgccgg atcgttagcg tcgacttcga cctgagtaac cagcgttgct
    12301 accggtttat taatgccccg agaaagcagt tcttctctta aggcgttttg tagatcgtaa
    12361 ccaatataac cctggcttaa ggcaacgcag acagacatcg gcagcattgg cgagtgcgct
    12421 tcagtttttg cagcagcttc aaaggcctgg ttaatcatgc ccacctgcgg cccgttacca
    12481 tgagtgacga taacttcatg tccttgggca attaaatcaa caattgcctg agaagtaatt
    12541 tttaccgctt tcatttgtcc ggccaggtca tcgcccagcg cattcccgcc cagggcgaga
    12601 acaattttct tactcatttt tcctcactca tcattcttga ttgtgtatag atccctgaca
    12661 gtggttagag cacgggaggg acaaacggtt tgcgtcgcag gaaacgacct cgcccggctt
    12721 tgcctgtaaa ggtgccgtca cagaaaatcg tttcaccacg ggataaggtt ctgacaatcg
    12781 cgccctgaca ggtaaaaccc tcccatggcg agtagtcggc gttgtcgtgg agatggcgat
    12841 gctgaatttg ttggctctga cgtgggtcga taatcaccac gtcgccatcg gaaccgggcg
    12901 ctaataatcc tttttgcggc cacaggccaa acagcctggc gggcattgcg ctggttaatt
    12961 caacaaagcg ttccggtgtt atacgtcccg tcatcacgcc actggaaaac agtaactgca
    13021 tgcggttttc cacaccgggt aagccatttg ggcagcgact gaaatcgcct ttagaaattt
    13081 gcaggcgttg agccatcgag aaggtgcagt gatcggtcgc caccacgtca atcgcaccat
    13141 cgctgatgcc acaccacagt ttgtcctgct cgcgtacgtt acgcagcggt gggctaagaa
    13201 tgaacttcat gccgtcttct gtatcgtaac tgcgttcgtc caacaggaga tattgtgggc
    13261 aggtttcaac ccagactggc tggtgattcg cacgggcaag acgcagataa tccagaccta
    13321 agccgttaga caggtgcacg atatagagcg gggcgttacc ggcaatttgt gccaggttaa
    13381 tcatgcgggc gatggcttcc gcttcgcatt ccagagggcg actcaaggca tgatagcgcg
    13441 gcgcggttaa ccctgcggcg ataaattccg cccgcttgct ggcgatagcc gcatcatttt
    13501 ccgggtgcac ggtggtcagc gcgccggatt catgcagacg gcgtaatgcc tgcaaaacct
    13561 cgtcatcgtt gagtttgtat tgataggtta aatagagttt aaaactgctc agtccttcct
    13621 cgaccatcat cgggatttcg tcgaggattg cgtgattaat gtgctggatc acaccgtgaa
    13681 agctgtaatc gatgaccgct ttatgggcgg cataaccacg ataaacctcc agttgatggc
    13741 gtaaccgaca gccgtttggg ccaaatccca tatggtcaat aatggttgtt gtaccgccac
    13801 acgcagctgc gcgggtaccg gtaaaaaaat catcacaact gcgcgcgatg ccgacatcaa
    13861 tattgaaatg cgtatggaca tccacgccgc cagggaaaac gtaacagcca gtggcatcaa
    13921 tttcttcata cgggagctgc ggcgaaatat tgttgcccaa ctggcgaaca atcccgcttt
    13981 caatcagcaa atcctgtttg gcttgtccat ctgcgttaac gacagtgccg tttttgatca
    14041 atacgcgcat agcaaactcc aaaaaccctc cggcgaaccg gaggggaata agtgaagagt
    14101 aattactctg ttgccagcca gcttaacggg attgcagcgt acatcgcggc acaggtcacc
    14161 aggtgagatt tccaggtttt ttcgttaggt gcatgcgctt caggttcttt acccgggcca
    14221 aagccgatca ccggaatgcc gtgacggccc atgatagata cgccgttagt tgagaaggtc
    14281 cacttatcaa caaccggcgc tttgccaaac agcccttcgt aggcattcac cagtgcttta
    14341 acggtgaagt gatcttcttc cactttccag gtcgggaagt agcattcggt tgggtaaacc
    14401 aggccagtcc aggacggacg gtcgtagttg tacatagaaa caaccgcgtt agctttctgt
    14461 actgcaggca gggcgcggat ttcgtccagc gcgccttccc aggtttcgcc ccaggtcaga
    14521 cggcggtcaa tagagacggc gcagctatct gctacagcgc aacggcttgg ggatgtgaag
    14581 aagatttcag aaacggtgag ggtgcctttg ccgaggaatt cgtcataacc cagacgttgg
    14641 gagagttctt gtaattcgcc aagaatcgga cccattttga aaatggcgtt gtcaccgcgt
    14701 tctggcgcag aaccgtggca gctaacaccc tgaacatcaa tacgaatttc catacgaccg
    14761 cgctgaccac ggtatacctg gcagtcggtt ggttcggtac tgaccacaaa ttccgggcga
    14821 atgccggatt gttcaataat gtactgccag cacagaccgt cgcagtcttc ttcctgcaca
    14881 gtaccggtaa ccagcagggt atattcatct tccagaccga ggtctttaat gattttaccg
    14941 gcataaacca tagatgccat gccgccttcc tggtcggaag taccgcgacc accgatcagc
    15001 tcatcagttt ccatgccttc gtacggatcg aagtcccagt ttttgatgtt gccaatgccg
    15061 acggtatcga tatgagcgtc cattgccacc agacgcgggc cgtggccgat ataaccgaga
    15121 acgttgccca tcgggtcgat ttcaacttta tcgaagccga ctttttccat ctcttcttta
    15181 atacgatgta ctacgcgttt ttcgtcgcag ctttcactgg gaatagcaac catgtcgcgc
    15241 aggaagcgag tcatgtccgc ctggtaatct tttgcttttt caagaatcag tttgaatgga
    15301 atattcttag ccattatttt tctccagatt ttttactccc cgttttgcag ggagtagttc
    15361 caatagggtg attaaggtgc tacagcgtgt ttgccttccc agacaacttc gcggtagtgc
    15421 ttcacgtcgg tgtcgccttc agtgctgata accagcacta cggcatcttt gttcagcgcc
    15481 agtttttcca tcaggctttg acgttgcggg tgataatgaa ccgctgcgag aacgcccaaa
    15541 ccgacagcgc cggattcacc ggagatgatg cgcgggtcgt tgccgtacgg attacccagc
    15601 acgcgcatac ctaatgcggc aacgctgtcc tggcaggaga tgaattgggt ggcgcagtta
    15661 cgtaggattt cccagcccag cgggttaggt tcgccacagg ccaggcccgc catgatggtg
    15721 gccatatcac cgccaacgtt gacgatgtcg cctttgacgc cggagcgata aatacagtca
    15781 gctttgtcag gttcaacaat aatgctgtgc agattttgcg ggctgtagac gtcgaccaga
    15841 taacccagca caccaccggc cattgctccg acaccggctt gcagcagaac gtgcgtcggg
    15901 gttacgccca tttcacgcat ttgctcgacg gcttcatctg ccagggttgc gtagccttgc
    15961 atgatccagg ttgggatttt ggtgtaacct tcccatgccg tgtcctgtac cacttcccag
    16021 ccgtgctgct gcgcgtgttg catggtcagg cgaacggtat cgtcatagtt catatccgtg
    16081 acgatgcact cggcaccgag gttcagaatg gcgtcaacgc gttcctgagc agaacctttc
    16141 ggcatgtaaa tcaccgcatt ctgtccgagt tgctgtgctg cccacgccac accgcgcccg
    16201 tggttgccgt cggtggtggt cgcgaaagtc attttttcgc cgatggcatt tttcaggtgc
    16261 tcaaatgaca gcgtttcgat atcaagatga tatttttcac acaataattg agcgatggcg
    16321 tacgcaccgc caagcatttt gaaggcgttc agaccgaatc gttttgattc gtctttgacg
    16381 agaattttct tcacaccaaa aaggtttgcg agatcgtcca gcgcacaaag cggtgttggg
    16441 cgataaccag ctattttctg gtggaactgg cgcgccagtt tggcctggct ttgcgaaaag
    16501 agcggtgatg tttcgccgtt gaaaaatttg ttatcggcga tatcaatctt caatgagaaa
    16561 acggacatat cctatccttt tggcctctgg aaatagaggg ccgaccagct gatcggcccg
    16621 aggggttatt tcacgcgttc ttgcgcttct ttcagcagtt gctcgagcag tgcacctggt
    16681 ttggcgtatt tacgggacag gatcatcgcg gcgatgatat aaggcttcca gctggcttct
    16741 ttgtaggtag cgatacggta tttttcgaat acgccttcag tcacttcccc ttctttgcag
    16801 gatacgccgc tgatatcagc tggcaggcag tgcatgtaca gggcttcgcc atcacgggtc
    16861 agttccatca tctcttcagt acaatgccag tctttgtgtt gcgcgttctg tgccagacac
    16921 tgtttttcca gtgcttttaa gccttcgtga tcgttagcgc gcagcaattc ggtacgctgt
    16981 tccatcactt tgtaaggtgc ccatgacttc ggataaacga tgtctgcatc tttgaatgct
    17041 tcttccatgc tggtgacctg acggaagcta ccgccagatg ctttagcatt gtttttcgcg
    17101 acttctacta catccgggat caggtcgtag ccttccggat gggccagggt gacatccata
    17161 ccgaagcgag tcatcagacc gatgatgcct tgtggtacag agagcggttt gccatagctt
    17221 ggagagtagg cccaggtcat ggcgattttt ttacctttca ggttttccag tgaaccaaag
    17281 tgttcacgta accacgcgag gtcagccatt gactgagtcg ggtggtcaat atcgcattgc
    17341 aggttcacta aagccggacg ctgtggcagt acaccctgct tgtaaccgtc atcaagtgca
    17401 gcgccaactt cacgcatata ggcgttgcct gcgcccagat acatatcgtc gcgaatacca
    17461 atagcgtcgg cgcagaagga gatcatattg gcggtttcac gcacggtttc gccgtgagcg
    17521 atttgtgatt tgccttcgtc gagatcttgt tgtgcaaggc cgagcaggtt aagcgcggaa
    17581 gcataagaga agcgggtacg agtggagttg tcgcggaata cggaaatacc taatccacta
    17641 ttaaagactt tggttgagat gttttcagca cgcagtgctt ttaatgctgc ggcaacgtcc
    17701 agtacttgtt tcagttcatc tggcgtctgt tcccacgtta acaaaaaatc tttctcgtga
    17761 aggtgagagg tcagcgaatt gatatcctta atcagctcat taacagtttt catcataaag
    17821 tcccggtagt ggaatgacag gcaaagtggt ttatttcact gtgacttgcc aaagtgattg
    17881 gtgttttcac agctatacct gggactaaca ataagtgtgc cagaattatc cacggggctg
    17941 taaaagacta atttgacgga taatgtgtgg ggttgatcac aaattgatgc cagcttcggg
    18001 ttgtttgtaa
//
LOCUS       DAZGVQ010000106        17886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000106 DAZGVQ010000000
VERSION     DAZGVQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.107"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(33..2480)
                     /gene="glgP"
                     /locus_tag="ACHLAG_003438"
     CDS             complement(33..2480)
                     /gene="glgP"
                     /locus_tag="ACHLAG_003438"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFP0656602.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="ACHLAG_003439"
     CDS             complement(2499..3932)
                     /gene="glgA"
                     /locus_tag="ACHLAG_003439"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFP0656603.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="ACHLAG_003440"
     CDS             complement(3932..5227)
                     /gene="glgC"
                     /locus_tag="ACHLAG_003440"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFP0656604.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="ACHLAG_003441"
     CDS             complement(5245..7218)
                     /gene="glgX"
                     /locus_tag="ACHLAG_003441"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFP0656605.1"
                     /translation="MTQLAIGKPAPIGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="ACHLAG_003442"
     CDS             complement(7215..9401)
                     /gene="glgB"
                     /locus_tag="ACHLAG_003442"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFP0656606.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9674..10777)
                     /gene="asd"
                     /locus_tag="ACHLAG_003443"
     CDS             complement(9674..10777)
                     /gene="asd"
                     /locus_tag="ACHLAG_003443"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP0656607.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10970..11563
                     /gene="yhgN"
                     /locus_tag="ACHLAG_003444"
     CDS             10970..11563
                     /gene="yhgN"
                     /locus_tag="ACHLAG_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFP0656608.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11620..12960)
                     /gene="gntU"
                     /locus_tag="ACHLAG_003445"
     CDS             complement(11620..12960)
                     /gene="gntU"
                     /locus_tag="ACHLAG_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP0656609.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12964..13491)
                     /gene="gntK"
                     /locus_tag="ACHLAG_003446"
     CDS             complement(12964..13491)
                     /gene="gntK"
                     /locus_tag="ACHLAG_003446"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP0656610.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLMVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13630..14625)
                     /gene="gntR"
                     /locus_tag="ACHLAG_003447"
     CDS             complement(13630..14625)
                     /gene="gntR"
                     /locus_tag="ACHLAG_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFP0656611.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDADLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14849..15544)
                     /gene="yhhW"
                     /locus_tag="ACHLAG_003448"
     CDS             complement(14849..15544)
                     /gene="yhhW"
                     /locus_tag="ACHLAG_003448"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFP0656612.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15667..16704)
                     /gene="yhhX"
                     /locus_tag="ACHLAG_003449"
     CDS             complement(15667..16704)
                     /gene="yhhX"
                     /locus_tag="ACHLAG_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP0656613.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADNSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            17037..17525
                     /gene="yhhY"
                     /locus_tag="ACHLAG_003450"
     CDS             17037..17525
                     /gene="yhhY"
                     /locus_tag="ACHLAG_003450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP0656614.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            17762..>17886
                     /locus_tag="ACHLAG_003451"
     CDS             17762..>17886
                     /locus_tag="ACHLAG_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFP0656615.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFT"
BASE COUNT         4427 a         4833 c         4447 g         4179 t
ORIGIN      
        1 accctttggc cccgttctat ttattagtga aattacaatc tcaccggatc aatatgccag
       61 atatgatcgg cgtactcttt gatagtacgg tcagaagaga agtagcccat attggcaatg
      121 ttcagcatcg cttttgcggt ccactcttcc tgaagctcgt agagttcgtc gactttatcc
      181 tgacaatcga catagctgcg ataatccgcc agaacctgat agtgatcgcc gaagttgatc
      241 agcgaatcaa ccagatcgcg atagcgaccc ggatcttccg gactgaatac accgctgccg
      301 atttgcgtca gcacctgatg cagctcctca tctttctcgt agtattcacg cggtttgtag
      361 ccctgacgac gcagttcttc cacttcttcc gcagtgttac caaagataaa gatattgtca
      421 gcaccgacat gatccagcat ctcgacattc gcaccgtcca acgtaccgat agtaagcgca
      481 ccgttaagcg caaacttcat gttactggtg ccggaagctt ccgtccccgc cagcgaaatc
      541 tgttcagaca gatcggccgc cggaatgatc aactgcgcca ggctaacgct gtagttcggg
      601 atgaacacga ctttcagctt gtcgccaatc tgcggatcgt tgttgatcac tttcgctacg
      661 tcattgatca aatgaataat gtgcttcgcc atgtaatagg ccgaagccgc cttaccgcca
      721 aaaatattca cgcgcggtac ccacttcgca tccgggtcgg ccttgatgcg gttatagcgg
      781 gtaatcacat gcaacacatt catcaattga cgtttgtatt cgtgaatacg tttgatttgt
      841 acatcgaaca acgcctttgg attcaccacc acattcagct gctgggcgat atactctgcc
      901 agacgctttt tgttctccag cttcgcctga tgcacagcgt gattaaccat tgggaaatca
      961 cagtgttgtt gcagctcatt aagcaggcta aggtcggtgc gccagttacg gcccaggtgt
     1021 tcgtccagca cggctgaaag cgatgggttc gctaccgcta gccagcgacg cggcgtcaca
     1081 ccgttggtga cgttggtgaa acgacccggg aagattttcg caaagtcggc aaacaacgat
     1141 tgcaccatca gattagagtg cagttccgat acaccgttaa ctttgtggct cacaacaacc
     1201 gccagccagg ccatacgcac acgacgaccg ttggattcat caatgatcga cgcccgtccc
     1261 agcagatcgg tatcgttcgg atactgttcc tgcaaggttt tcaggaaata gtcgttgatt
     1321 tcaaagatga tctgcaggtg acgcggcaga attttaccca gcatatcaac cggccaggtt
     1381 tccagcgcct cgctcatcag cgtgtggtta gtgtaggaga agacctgaca acacacctca
     1441 aacgcgtcgt cccagctaaa ttggtgctca tcgatcagca gacgcatcat ctcaggaatc
     1501 gacagtaccg gatgggtatc attgagatga atcgcgattt tatccgccag gttatcgtag
     1561 gttttatgca actgataatg gcggcttaaa atgtcctgaa tggtcgagga aaccaggaag
     1621 tattcctgac gcaggcgcag ctcacgcccg gagtaggtgg agtcatccgg atacagtacg
     1681 cgagatacgt tctcggagtg gtttttatct tccactgccg cgaagtagtc accctggttg
     1741 aatttaccga ggttaatttc gctactggct tgcgcactcc acaaacgcaa cgtgttggtc
     1801 gcgtcggtgt cgtaaccagg gattatctga tcgtaagcga ctcccagaat ctcttcggtt
     1861 tcaatccaac gcgttttttt accttcctgc tgaatgcgac caccaaaacg gactttatag
     1921 cgcgtgttgt ggcgtttgaa ttcccacggg ttaccgtatt ccagccagta gtctggcgac
     1981 tctttctggc taccgttaac gatgttctgt ttgaacatac cgtagtcata gcggatgccg
     2041 taaccgcgcc ccggcaaccc taacgtcgcc agagaatcaa ggaagcaagc cgccagacgt
     2101 cccaggccac cgttaccgag gcctgggtca ttttcttcat caatcagctc ttcgagattt
     2161 aaccccatcg cttccagtgc gccctgtaca tcttcgtaaa ttcctagcga caacatggcg
     2221 ttggagagcg tacggccaat caaaaactcc atcgacaggt agtaaacctg acgagtttct
     2281 tgcgacaact gggcacggtt tgaacgtaac cagcgctcca cgagacgatc gcgcacagca
     2341 aataacgttg cgttcagcca ttcatgttta ttggcgacga ccgggtcctt tccaatcgta
     2401 aacatcagct tgtaagcgat agagtgctta agagcttcta cgctaagcgt gggcgatgaa
     2461 tatgtaaacg gagcattcat ataggcgttt cctgaaaact atttcaagcg atagtaaagc
     2521 tcacggtacg acttcgccgc gacctgccag ctaaaatcca ttgccatagc ctgacgttgc
     2581 acaaaccgcc acagtgaagg acgggaccac agtacaaaag cacgtcgaat agcccgtaac
     2641 agcgaccagg cattactatc ttcaaagaca aacccactgg cgacgccatc tgcaaggttc
     2701 tcaagagaac agtcagaaac cgtatcagca agcccaccgg tgcgccgcac taacggcagc
     2761 gtaccgtact tcaatccata aagttgcgtt aagccgcacg gttcaaaacg gctgggcacc
     2821 agaatgacgt ccgcgccgcc cataatgcga tgcgaaaatg cttcgtgata gccaatctga
     2881 acgcccacct gaccggggta ttccgctgcc gccgcaagga aaccttcctg cagcaccgga
     2941 tcgcccgcgc cgagtagcgc cagctgcccg ccctgctcca gaagacccgg taaggcttcc
     3001 agcaccaggt cgagaccttt ctggctggtc agacggctca ccactgcaaa aagcggcact
     3061 ttatcgtcaa ccttaagccc cattgcgatt tgtaactggc gcttattttc cgctttatct
     3121 tccaacgtat cgcgggtgta acgcgaggcc aacagtaagt ccgtctctgg actccagatt
     3181 ttctcgtcca cgccgttcag tacgccggaa agacgccctt cacggtgacg ctgttgcaac
     3241 agaccttcca taccgtaggc aaactgcggt tcggtgatct cgcgagcgta ggttggactg
     3301 accgccgtaa tgtgatcggc atagtacaga ccggccttca ggaaagagat ttgtccgttg
     3361 aattccagcc catgaatatt aaagaatgac catggcaatt ggatgtcatt catatgatgt
     3421 gcataaaaca tgccttgata ggccaggttg tgcacagtaa acaccgactt cgccggacgc
     3481 ccgcgcgccg ccagatacgc aggcgcaagg cctgcatgcc agtcgtgcgc atgcaccaca
     3541 tcaggacgcc agaatgggtc aagcccgctg gccatttctg ccccaaccca ccccagcagc
     3601 gcaaaacgca atacgttgtc ggtataggca aataagttgg tatcgtgata cgggcttccc
     3661 ggacgatcat agagatgcgg cgcgtcaatc aggtatatgc caaccccgtt gtaatgaccg
     3721 aacaacagcg tgatatgtcc ggcgaaggta tcacgacggg atactacctg cgcatcggta
     3781 acgccacggc gaatatcggg aaatgcaggc aacagtacgc gagcgtcaac gccgtctgcg
     3841 atttgtgctg cgggtaatgc cccaataaca tcagccagac cgccggtttt aagcagcggg
     3901 aacatctctg aacatacatg taaaacctgc attatcgctc ctgtttatgc cctaacttcc
     3961 gtagcatttc gcgcgttacc agcacgatgc cttcttctga acgatagaaa cgacgtgcat
     4021 cttcctctgc gttttcacca atcaccatgc cttccggaat aacacaagca cgatcgatga
     4081 cgcagcggcg cagacggcac gagcgaccta cccatacttc cggtaacaat acggcggaat
     4141 caatgttgca gaatgaattc acgcgaacgc gcgagaacag aacggactgc accaccaccg
     4201 aaccggagat cacacagccg ccggatacca gtgagttaag ggtcatcccg tggctaccgg
     4261 agcgatcctg cacgaatttc gctggcggta atgattcatt gtaggtgcga attggccaat
     4321 tgcgatcgta catatccagt tccggcacca cagaggccag atcgaggttc gctttccagt
     4381 aagcttccag cgtacccaca tcgcgccagt acggctcggc atccgggtcg gattgtacgc
     4441 aagagagcgg gaacgggtgc gcataggcca gaccggcttc ggtgatcttg ggaatcaaat
     4501 ctttgccaaa gtcgtggctg gagttctcat cacgatcgtc ttcttccagc agttcataca
     4561 gatagtcggc gtcaaagacg tagataccca tactcgccag agatttgctc ggatcgttcg
     4621 gcattgacgg tgggttagca ggtttttcaa cgaattcgat aattttatcg ttctcatcaa
     4681 ccgccataac gccaaatgcg gaggcttctt caatcggtac tggcatacaa gcaacggtgc
     4741 aacgtgcgcc tttttcgacg tgatcgataa gcatacgcga gtagtcttgc ttgtagatat
     4801 ggtcgcccgc caggatcacc acgtattccg ctttataacg gcggataatg tcgaggtttt
     4861 gggtgaccgc atctgcggtg ccgcgatacc agttttcccc tttcattctc tgctgtgctg
     4921 gcagcagatc gacaaactcg ttcatttctt cattgaagaa tgaccagccg cgctgaatgt
     4981 gctgcaccag agtgtgggac tggtactggg tgatcacgcc catacgacgg atcccggagt
     5041 tgatgcagtt agacagcgca aagtcgataa tgcggaactt accgccgaag tgtacggccg
     5101 gttttgctcg cttattggtt aaatccttca ggcgggtacc acgtcctccc gccagtatca
     5161 gggcaacaga tttcaatggc agctggcgcg ccaacattaa gtgatcgttc ttctctaaac
     5221 taaccatgac taactccttt tttatcatct ctggaacaca cacaatccgt gtgcaggtcc
     5281 ctgccagaca gccgtaatca ctgggttatc ctctccagcg aatgggggaa tggcgtgcca
     5341 ctccccagca ggtaaaacaa tctctgttac ctcaagcgtg gcgttaattg cgatcaaaaa
     5401 gcgatccgag agcagaattt gcagctgttt cggcccgttt tgccactcat ccgtgcttaa
     5461 aggttgagca tatcgattta gccaacggac attgccgtcg ccttcttccc accagcgatt
     5521 ctccaccaaa gcgggaatgc gcttgcgcag atggattaac gcggcggtaa atgcggttaa
     5581 accactgctt gcctgcgacc agtccaacca ggttaattgg ttatcctgac agtaggcatt
     5641 gttattgcca tgctggctgt gaccatgttc gtcaccggcc agtaacatcg gcgtaccctg
     5701 ggagagcaac aacgttgtta acagggcgtg aatgctgtcg cgccgccgtt caaccaggtc
     5761 aagagaaccg cctaaccctt ctttaccatg attgttactg taattgttgt tggtcccgtc
     5821 gcgattttct tctccgtttg cttcattgtg tttatggttg aagcaaacgc agtcgcgaag
     5881 cgtaaaaccg tcatgcgcgg tgacgagatt aatcgcggca ctcggcagac gaccattacg
     5941 tttaaaaaca tcgctggagg cagcaaaacg cccggcaaac gcccccagag gcaaatcata
     6001 atgtagccag aaacgacggg cagcatcgcg gaaatgatcg ttccactcgg caaacagcgg
     6061 cgggaaattt cccacctgat aaccaccagg agcgatatcc cacggttcag caattaactt
     6121 cacctgcgag agcaccgggc agttctggat agcggtaaac aacggcgcat cctgacggaa
     6181 ctctggcgta cggcccatga ctgccgccag atcaaagcgg aaaccatcga cgtggcaggt
     6241 ttctacccaa taacgcaggc aggcgctggc ataatccacc accgccggat gactcaaatt
     6301 gagcgtgttg ccgcaaccgg tccagttgtg ataatcgccg tcttctctta tccaataata
     6361 gctacggtta tcgatcccac gcagcgagaa taacgggccg tcgaggtcca gttccgcact
     6421 atggttgagc acgatatcaa gaatgacttc gatacccgct ttatgcagtg ctttgattgc
     6481 atcgcgaaac tcatccagcg ccgtttctgg cgagcaggca tacgccggat gcagcgcaaa
     6541 catcgccacc gggttgtaac cccagtagtt acttagcccc atgcgttgca gacgtggttc
     6601 actggcaaac tgcgccactg gcagcagttc cagcgcggta atgcccaatt gtttcaaata
     6661 gttgatcatc accggatgcc cgagggcttt ataagtgcca cggatctcga ccgggatctc
     6721 cgggtgcaag tacgttaatc ctttgacatg ggcttcataa atgatggtgc tgccccacgg
     6781 cgtgcgcggc ggggcatcat cttcccagtc atagtgatca accaccacta cgcatttcgg
     6841 cgcaatggcg gcgttgtcgc gatagtcagg ttcattatga ccggcgtgca gcagcgggtt
     6901 atctttaaac tccccctcaa tttgccgcgc gcaaggatca atcaacaact tcgccgggtt
     6961 aaagcgatgc ccctcggcgg gttgccaggg gccatgaacg cgataaccat aacgcaaacc
     7021 cgggcgcgca tccggcagat aaccgtgcca aatgtcgcca ctgtgccctg gcaagtcata
     7081 gcgatgttcc tggccattgg cgtcaaagac acacagttct acccgctcgg catgagcgga
     7141 gaaaagtgtg aagttgacgc cctgaccgtc gtaatgcgcg ccgatgggag cgggtttgcc
     7201 aatggcgagt tgtgtcattc tgcctcccga accagccaga tagtggccag cggtggtagc
     7261 gtcaggctta gtgaatgctg acgaccgtgg ctggcaatct catcgctgtg taccgtgccg
     7321 ccattgcctg cattactgcc gtgatagtgc atggaatcgg tattgaggat ttcacgccat
     7381 ttgcccggct ggtttatgcc gaagcgataa tcatgacgcg gcaccggcgt aaagttactg
     7441 gcaacgatga tttcgttacc ctctttatcg cgacgcacaa agatcagcac cgagcgttct
     7501 ttgtcatcca ccaccagcca ttcaaagccg tacgggtcaa aatccagttc atgcattgct
     7561 ttatggtggc ggtaggtgag gttcagatcg cgcaccagac gctggacacc gtggtgccag
     7621 ttatcgccgc cttccaacag atgccagtcg aggctggcgt catggttcca ctcgcggccc
     7681 tgggcaaatt cgttacccat gaacagtagt ttcttgcccg ggaatgccca catccagcca
     7741 tagtaggcgc gcaggttcgc gaatttctgc catgcgtcgc ccggcatgcg gtcgagaatc
     7801 gattttttac cgtggaccac ttcatcatgc gacaacggca ggacgaagtt ttcagtgtag
     7861 ttgtagagaa tcccgaaggt cagtttatcg tgatgatact gacgataaac cgggtcgagc
     7921 ttcatgtagt ccagggtgtc atgcatccag ccgaggttcc acttgtacca gaagcccaga
     7981 ccgcccatat cctgcggacg agaaacgcca gggaaatcgg tagactcctc agccattgtc
     8041 accgcaccgg aaacctgctc accaagaata cggttggtat tacgcaagaa ttcaatcgct
     8101 tcaagattct cgcgcccgcc aaattcgttc gggatccact ccccctcttt acggctgtag
     8161 tcgcgataaa tcattgacgc caccgcatcg acgcgcagcg catcaatacc aaaacgttca
     8221 atccagtaaa gcgcgttacc gacgaggaag ttactgactt cacggcgacc atagttgtag
     8281 atcagcgtgt tccagtcctg atgatagcct tcacgcggat cgctgtgttc atacaagttc
     8341 gtgccatcaa attcggcaag cgcaaagtca tcagtcggga agtggcctgg cacccagtcg
     8401 agaatcacgt tcagaccagc tgcgtgtgcg gcatcaatga aataacggaa gtcgtcgcga
     8461 gtaccaaaac ggcgggttgg tgcatacagg ccggttggct gataacccca actgccatcg
     8521 aagggatgct cgttaatggg cagtagttcg aggtgggtaa agcccatcca tttagcataa
     8581 ggcaccagtt gatcggccag ctcgcggtag ctcaaccaga aattgttgtc ggtgtggcga
     8641 cgccaggaac ccaggtgaac ttcataaata gagattggcg catcaaactg attcgctttt
     8701 ttgcgctctt cagtctgtac aaccttttcc ggcagcccgc aaataagaga cgcggtttcc
     8761 gggcgcattt gcgcttcgaa ggcataaggg tcggacttca gacgcaagtt gccattggca
     8821 tcaatcatct cgtatttata gagctgaccg ttatgcgccc cagggataaa cagttcccag
     8881 atgccgctct ctttacgcag gcgcatcggg tgacggcgac cgtcccagta gttgaattgc
     8941 ccaaccaccg agacccgacg ggcgtttgga gcccagacag agaaacgcgt acctgtgacg
     9001 ccatccatag tatctgcatg cgcgcctaag gtttcatacg ggcgcaggtg agtaccttca
     9061 gataatagcc aggcatccat ttcctggatt agcggaccaa aacggtaagg atcatcaatc
     9121 aggttttgct gaccatgcca gacaacagcc aactgatagc ggaaaaaatt cttacgtcgc
     9181 ggaatgacgc cgctaaagaa tccccgtgag tcgagacact ccagttttgc gagtttgcgc
     9241 ccggttttcg gttcaatcac ccacacatcg gtagcgtcgg gtaaaagggc acggacttcc
     9301 agtcccgcgg tggttttatg cattcccagt acggaaaaag gatccgcaaa atggcctgca
     9361 attagcgcgt taatcacgtc tctatcgata cgatcggaca tgcttgtctt cctgttttat
     9421 tgtgtcaccc catccaattg gatttttgac ttctggttgt gacatttttt tgacctgaac
     9481 ggcgcagcac tctgtgcatc ctctctgcgt cgtcctcact tcaggtaagg ctgtgaatac
     9541 tcatgtattc agccaccctt aaagaatagc caatgctcta tttaactccc ggtaaatcat
     9601 gaaacatctg cgcttactcc tgtattacgc actaacaggg gcggcatcgc gccccagatt
     9661 taatgaataa agattacgcc agttgacgaa gcatccgacg cagcggctcc gcggcccccc
     9721 acagcagctg gtcgcccacg gtaaaggctg acaggaactc tggtcccata ttcagcttac
     9781 gcaggcggcc taccggcgtg gtcagcgtgc cggtaacggc agctggggtt agctcacgca
     9841 tagtgatttc ccgatcgttc ggaacgactt tcgcccacgg attgtgcgca gccagcagtt
     9901 cttccacggt cggaatagac acatcttttt tcaatttaat agtgaatgcc tggctgtggc
     9961 agcgcaatgc cccgacacgc acacataaac catctaccgg aattacggaa gatgtgttga
    10021 ggatcttgtt ggtttccgcc tgccctttcc actcttcgcg gctctgaccg ttatcgagct
    10081 gtttgtcgat ccacggaatc aggctacccg ccagcggcac gccaaagtta tccaccggca
    10141 gctcaccgct acgggttaag gttgtgactt tgcgttcgat atcgagaata gcagaggacg
    10201 gggtcgcgag ttcatctgcc acatggccat acagatggcc catctgggtt aataactcac
    10261 gcatatgtcg cgcaccaccg ccggaagcgg cttggtaggt tgcaacggac acccaatcaa
    10321 caagatcatt ggcgaataaa ccacccaacg acatcaacat caggcttacg gtacagttac
    10381 cgccaacaaa agtcctgatg ccattattta atccgtcggt aatgacgtcc tgattgacgg
    10441 ggtcaagaat gatgatggcg tcatctttca tgcgcagaga cgatgctgcg tcaatccagt
    10501 aaccttgcca tccgctttca cgaagctttg gatagatttc gttggtataa tcgccgccct
    10561 gacaggtcac aatgatatcg agggccttta gcgcctccag atcaaaggca tcctgaagtg
    10621 tgccagtggt tccgccaaaa gacggcgcag cctggccaag ctgagaagta gaaaagaaga
    10681 cagggcgaat ggcgtcgaag tcgcgctctt caaccatgcg ttgcatgaga acggagccga
    10741 ccataccgcg ccagccgata aaaccaacat ttttcataag cgtttttttc ctgcaaagat
    10801 gtgtgctgta taaatgtgcc ggtctcctct tggcacatct ttcaccatac aaaaagcagc
    10861 caaagtcgca agtgaaatta atcaatgata gcgaagccat cagtaatgcg acttatcctg
    10921 ctttgttagc acgcagaaag tccgcggcaa ttatcaggga atttgagtta tgaatgaaat
    10981 catttctgca gcagttttat tgatcctgat tatggatccg ctcggaaacc tacctatttt
    11041 catgtccgta ctgaaacata ctgaaccgaa aagacggcgg gcaatcatgg tgcgagagtt
    11101 gcttattgct ctcctggtga tgctggtgtt cctgtttgcg ggtgagaaaa ttctggcatt
    11161 tcttagccta cgagcagaaa ccgtctccat ttctggcggc atcattctgt ttctgatcgc
    11221 cattaaaatg attttcccca gcgcttcagg aaatagcagc gggcttccgg caggtgaaga
    11281 gccatttatc gtgccgttgg caattccgtt agtcgccggg ccgactattc tcgccacgct
    11341 gatgttgttg tctcatcagt acccgaatca gatggggcat ctggtgattg ctctgctgct
    11401 ggcctggggc ggcacctttg tcatcctgct acagtcttcg ctatttttac gtctgctggg
    11461 cgagaaaggg gtgaacgcac ttgaacgcct gatgggattg attctggtga tgatggcaac
    11521 ccagatgttc ctcgacggca ttagaatgtg gatgaagggg taacgaggag agtttttcga
    11581 aaagaacccg gtagcgtcac aactaccggg cgaacaaact taactcaaca gctggaacgc
    11641 aatcatccca acgatggcac cgacagtgcc gaggatggtt tccatcatgg tccaggtttt
    11701 cagcgtttcg gcttcggtcg cgccggtaaa tttaccgaac aaccagaaac cggcgtcgtt
    11761 aacgtggctg acaacaatcg aaccaccagc gatacaaatc gacagcgccg ccatttgcgc
    11821 accggagtag ttcagttgtt caataaccgg catcaccagt cctaccgccg ttaaacaggc
    11881 tacggtggca gaaccctgaa tgatgcgcac tgcagctgcc agcacgaagc aggtgatggc
    11941 tatcggcagg cccatgccgg ttaacgcttc gcccagtgcc ggacctacgc cagagtcaac
    12001 cagcacctgt ttgaacacgc cgcctgcacc aatcaccagc agaatgatcc ccgccggttg
    12061 cagcgcgtga ccgcaaatct ccatcacttt gtcttttggc atgccctgac gcattgccag
    12121 gccataaatc gctaccagac aagcaaccag aatcgcggta aacggatgac caataaactc
    12181 gaaccattcg taagcggtag agccttctgg cacaaaacgc gcggcaatgg ttttcagccc
    12241 caccagcacc agcggcaaca ggatcagcga caggctgaat ccgaaagatg gcattttgcc
    12301 ttcgccgaga tgcggttcgc tgatgtcgtc aggaatatgc aactccacgt agcggctgat
    12361 gaaattaccc cacagcggcc cggcaataat cattcccgga attgccgcac acaggccaat
    12421 caggatcatc cagccaaagt cggcattcat ctgcgatgcc agcagcatcg gcgctggtcc
    12481 aggcaccaga aatgccgccg ctgccgccac gcctgcaaat aatgggatta ccagcttcac
    12541 caggttcgta ccggtgtggc gcgccattga gaaagcaacg ctaatcagca gaactatcgc
    12601 cacttcaaag aacagcggta gcgcacagac cagccccgca aggccgatgg cataatgcgc
    12661 gcggctgtga ccgaaggatt tgagcatttt gacggcaatc tgatcgactg cgccggtttc
    12721 atgtaagatc ttgccaaaca tagctcccag ggcgacaacc accgccagga agccgagggt
    12781 gcctcccatc cctttttcca tcgtcgctgc gattttatcg agcggcatac cggaaaaaag
    12841 gccagccccc atggacacca ccatcaaagc caggaaagcg tgcatacgcg ccttcatgac
    12901 taaaaacagc agcagtaaaa cagaccctac tgctgttaaa acaagcgtta atgtagtcac
    12961 tacttatttg ccttttttaa taacctcaat ggtgcttgcc acaacacctt ccagcggttg
    13021 atcgatatcc accatcagta catcggtttc gtccgcaccc ggctcctgca gcgtttcaaa
    13081 ctgcgtcacc aacatttggg ttttaaagaa atggcctttg cgcgctttca ggcggctttc
    13141 aatcacatca aaatcgcctt tcaggtagat gaaagagaga ttcggattac cttcacgcag
    13201 caagtcgcga tagtgttttt tcaatgcaga acagacgatc agtgacactt tattagtgcg
    13261 ctgcatagca aacgcggcgt cgttcagcgc ctgcaaccac ggtttgcgat cgtcgtcatt
    13321 cagcggttcg ccagacgcca ttttttcgat attgcgccgt ggatggagga aatcgccatc
    13381 aagaaacgcg gcatgaagtt gatgcgccac ttcactggcg accgcagatt tgccgctgcc
    13441 tgagacgccc atcaagacgt aaatgtggtg atcatggtta gtcgtgctca aagtggtgcc
    13501 cccacaatac aagaattaaa aatgttacgg gtaactgtta tcggtaacat tgtccggccg
    13561 gacaatatca gaagcaatat ccatccgtgc cttaagtgta taagtgtgag ctacttcaaa
    13621 tttgtgggct taaatagatc cgcccggtga caaggtgaaa cctaaatcta acattttcgg
    13681 tgtcacagat tcgccacgaa tacgcgccag caggcgttca gcgccaatac tgcccatccg
    13741 ctcacgcggc gtcagcacgc tcgccagtcg tggctccatc acctgaccaa tgtcatgacc
    13801 gtggaaaccg gcaatcgcca tatcgtcagg aacttttaac cccagacgct gacattcaaa
    13861 cgccgcgccg accgccagat catcattagt acagaacacg ccatccagct gcggatattc
    13921 ccgcctcgcc tggcgaatca gttcaatacc ggaagagtaa gaagaagatt gctcaaccat
    13981 cacgctatat ggcaccaggt ctgcatccag catcgcctgt tcgtatccct tctgtttgat
    14041 gatagtacgt tcgtcgagac gtgcgccgag ataagcaatg tggcgatgcc cgcgagcaat
    14101 aatggcggtg gtcatctggc gtgccgcttc aaagttatca aaaccgacgg caatatcgag
    14161 gcacggcgac ttgctgtcca tcaactccac caccgggatc cccgccactt caatcatctt
    14221 taaggtgcgt ggcgtgtgag tacgttcggt gaggatcagt ccatcgatat tccatgagag
    14281 catggattcg aggcgttctt gctccatttc cggtttataa ccgtagtgcg ccagcatggt
    14341 ttgataaccg tgcgcgtcgg tgacgctttc gattccgcgt aatacttccg cgaaaacctg
    14401 gttggtgaga gaaggtaaca ggacgccaat cgcccggctg gtggcgttag agaggatatc
    14461 gggcgcacga ttgggaatat aacccagttc atcaagagcg gcggcaatct tgccgcgtag
    14521 agcgacggaa acctgctccg ggttacgcaa aaagcggctg accgtcattt tggtcacgcc
    14581 tacacggtca gccacatcct gaagtacggg tcttttcttt ttcatcgtcc tgaaggtaca
    14641 aaagagatag attctcttag tttaacacgg acgcatcaga accttcccgg ggaaaacagt
    14701 gcttgtttac aattattaga tatacactaa atcaattgca tcgcattgtg ctaacgacgc
    14761 ggcaaaaccc cggaagcgtg catcagcacg cgaccggagc gagcgaggaa gcctgcaaaa
    14821 aggcaggctt caaaaatgcc gttgagtttt aaaccggcgg cagatcgaac agtaacactt
    14881 cgctgtcgct atccgcatgg atggagattg cctgctcatc ccagattgcc agaccatcgc
    14941 tggtcgaggc tttcacgccg ttaatggtga cattgccttt caccacctgg atccagacgc
    15001 ggcgttcagc ggcaatctga tgcaccgact gctcatcttt cagcaacgcc caacgataca
    15061 gttccatatc ctgatgcact ttcagcgaac catctcgcgc atccggcgag agcaccagct
    15121 gtttgccctg cacggcatcg aagcgacgct gttcataacg cggcgtaatg ccattttctt
    15181 caggcatgat ccagatctga tacagatgca gacgctcggt gctgcttggg ttgtactctg
    15241 agtgacgaat acccgtaccc gcactcataa tctggaactc acccgccgga acctgctctt
    15301 tattgcccat gctgtcctga tgctcaacag taccttccag cacgtaggtc aaaatttcca
    15361 tatctttatg cgggtgagtg ccgaagccct gccctgcttc aatcacgtcg tcgttaatca
    15421 cgcgcagcgc ggagaagccc ataaagttcg gatcgtaata gttggcaaaa gagaaagtat
    15481 gccaggagtc cagccagcca tgatttgcat gaccacgttc atttgctttg cgtaagtaga
    15541 tcatttgtat tcgccccctg aatgatttcg atgggcttag tgtggacccg atctgcctag
    15601 gatgatagag ggtgaaaact gacccctctg ttcaaaaaaa ttgaactatt cgaggggcca
    15661 tcaaacttac ttagcgagag ttactgtgga gggagaggct tgctcaaatc cgcgttcaag
    15721 gatttccaga ttggtaagaa cttcagattc cttgacgtaa tttggcgcac cgtgggtgat
    15781 ggtttgatac aacgcatcat aaacgcgccc gtaatcgccc atctccggct tcatctcttc
    15841 tctgaccgtc acgccctcgt cattgacata ctccagcaca ccgaccgaat tatccgctgc
    15901 gaatcccggt tcgcccggca taatattagc cttcaggctg gtttcctgct ggtcgatacc
    15961 gtatttaata aacgaacctt tcttaccgtg aacgataaat ttcggataat cgattttcac
    16021 cagatggctg gttttgacga tggctttcag gtcgccataa aacagttgcg cttcaaaggt
    16081 gtcgtcagga ttggctttat tacgcaggct gcggatgtca taagcgacgt gatccgggcg
    16141 accgaacaga gaaataatct ggtccatcgt atgcacacca aggccataaa acgcgccatc
    16201 ctgcggcagc ccaggtttgg tttctgccac cgggcggtaa tagtcaaaat ggctttccac
    16261 ttcaacaatc tctcccaact tgccactttc aatcgctttt ttcgctgtca ggaagcagga
    16321 gtcaaagcga cgattctgat acggcgtgac ggtcagccct ttgcttttcg ccaacgcaaa
    16381 cagctctttc gcctgcgcaa gtgtcggagt gaacggtttt tcgaccagca catttttccc
    16441 ggcttccagc gcgcgcttcg cgtactcgaa gtggctgtcc gcgtgggtgc agacaacaac
    16501 cagcttaaca tcgggatcgt ttagtacttc gtcgagatcg ctggtgaaat ggatatggga
    16561 ataaatggga gcctgttctt ccggcttcgc atggcgacga aaaatatgcg cgacatgcca
    16621 actatccttg cggttaagta catacggcag atggtaacgg gtggtgcttt tgccgaagcc
    16681 aataaaggcg cagttgatga ccatgatttc gtccttttta aggtggttat ttacacctta
    16741 gcgcaaagcg gacgtggttc ctactggagt gcgcataacg aacacaagca ctcccgtgga
    16801 taaattgaga acgaaagatc aaaaaaaaag ccagcacccg gctggctaag taatactgga
    16861 agcaatgtga gcaatgtcgt gctttcaggt tctccgcgag ggtcttcctg atcgcgagac
    16921 aataataatc attctcattc gcacttgtcc aacacttttt gcaaaaaaat gcatttgact
    16981 cgcatttgaa agtcaatgat gttgaaaggg acatttaccc caaagaggac aaaggaatga
    17041 gtgagatagt aatacgccac gcagaaacac gggattacga ggccatcagg cagattcacg
    17101 cccagccgga ggtgtattgc aacacactac aggtgcctca tccttccgat catatgtggc
    17161 aggagcgact cgccgatcgt cccggcatca agcaactcgt cgcctgtatt gatggagacg
    17221 tcgtgggcca tctcaccatt gacgtgcaac agcgcccacg ccgcagtcat gttgccgatt
    17281 ttggtatctg tgtcgactct cgttggaaga accgcggcgt cgccagcgcc ctgatgcgag
    17341 agatgattga aatgtgcgac aactggttgc gggtagatcg cattgaacta accgtgtttg
    17401 tcgataacgc cccggcaatt aaggtctata aaaaatacgg ctttgaaatt gaagggactg
    17461 gtaagaagta cgcattgcgt aatggtgaat atgtcgatgc atattatatg gcgcgggtga
    17521 agtaagatag tgcccttttt ctgagatgga aaaagggtgt cattcaaaat cgacatacct
    17581 tcctttaagg tatttatttg cccaatacat atattgagat ttaactcata attacttcct
    17641 aaagtgtaat attttatttt ttaatatata cgcctacaat ttcctggagt aaataaataa
    17701 caattaacaa gcataatatt gccattgata aaatagcatg ccataaaagg acttttcagg
    17761 gatgagtaat attgtttacc tgacagtaac gggagaacaa caaggaagca tctccgcagg
    17821 ttgtgggact tctgagtcta caggtaatcg ttggcagagc gggcatgagg atgaaatatt
    17881 tacatt
//
LOCUS       DAZGVQ010000107        17774 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000107 DAZGVQ010000000
VERSION     DAZGVQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.108"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            106..2550
                     /locus_tag="ACHLAG_003452"
     CDS             106..2550
                     /locus_tag="ACHLAG_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP0656616.1"
                     /translation="MNADKEEIALYYEAKNDKVRKRLGIKGGFYWRTAKKLSVAISRG
                     VVAMDDAGFDEEDFKKPVRVNLPIVNDLPPEGVFDTEFCNRYEKGGEDGITMIFIAPS
                     PSVQDKPASTDNTNVNGEDMAEIEDNMLLPISGQELPIRWLAQHGSGKPVTHVSREEL
                     QALHIARAEELPAVTALAISHNTKLLDPLEIRDLHKLVRDTDKVFPNPVNSSLGLMTA
                     FFEAYLGADYTDRGLLTKEWMKGNRVSRISRTPSGANAGGGILTDRGEGFVHDDASVA
                     RDVATGVLARSMDVDIYNLHPAHAKRIEEIIAENKPPFSLFFSKFITMPGHKDYSRAI
                     VVASVKEAPIGIEATPHRVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMPQRVTKE
                     GKQDDEEKPQPTGAMADEQATTEAVEPDTTEHNQDTQSMDAQPQINSVDAKYQKLRAE
                     LHEARKNIPPKNPVDADKLLAASHGEFVEGISDPNDPKWVKGIETRDSVNQNQHESEQ
                     NDQKAEQNSPNALQNEPETKQPEPEEQQEPEKVCTACGQTGDGNCPDCGAVMGDATYQ
                     ETFNEESQDEAREKDPEEMESAVYPNKECTEGDQHANGNNETGETENPLIKVNGHHES
                     TSTSRLWHHLMIDLETMGKNPDAPINALAGKFFDPATGEMGPEFSKTIDLETAGGVID
                     RDTIKWWLKQSREAQSAILTDEIPLDDALLQLREFIDENSGEFFVQVWGNGANFDNVI
                     LRRSYERQGIPCPWRYTNDRDVRTMVALGLVMDFDARSVITFEGERHNALHDARYQAK
                     YVSAIWQKLIPSQADF"
     gene            2612..2881
                     /locus_tag="ACHLAG_003453"
     CDS             2612..2881
                     /locus_tag="ACHLAG_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP0656617.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            2850..3968
                     /locus_tag="ACHLAG_003454"
     CDS             2850..3968
                     /locus_tag="ACHLAG_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFP0656618.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDSRLKN
                     GDIAATTHKEKVRMAAYLVSRLGNHPLKELEVRDFALILDEWMDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRSRLTLEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNGHLHITQGKTGMRLALPLTLRCDEIGVSLKEVIDGC
                     RDRMLSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGVDTKTLLGHKVQATTDRYNDTRGQEWVKLVI"
     gene            4135..4929
                     /gene="potC"
                     /locus_tag="ACHLAG_003455"
     CDS             4135..4929
                     /gene="potC"
                     /locus_tag="ACHLAG_003455"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFP0656619.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            4926..5972
                     /gene="potD"
                     /locus_tag="ACHLAG_003456"
     CDS             4926..5972
                     /gene="potD"
                     /locus_tag="ACHLAG_003456"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFP0656620.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            6030..6491
                     /locus_tag="ACHLAG_003457"
     CDS             6030..6491
                     /locus_tag="ACHLAG_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFP0656621.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            6488..7276
                     /locus_tag="ACHLAG_003458"
     CDS             6488..7276
                     /locus_tag="ACHLAG_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP0656622.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(7415..8236)
                     /gene="cobB"
                     /locus_tag="ACHLAG_003459"
     CDS             complement(7415..8236)
                     /gene="cobB"
                     /locus_tag="ACHLAG_003459"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFP0656623.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(8252..9163)
                     /gene="nagK"
                     /locus_tag="ACHLAG_003460"
     CDS             complement(8252..9163)
                     /gene="nagK"
                     /locus_tag="ACHLAG_003460"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFP0656624.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(9192..10436)
                     /gene="lolE"
                     /locus_tag="ACHLAG_003461"
     CDS             complement(9192..10436)
                     /gene="lolE"
                     /locus_tag="ACHLAG_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFP0656625.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGVNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(10436..11137)
                     /gene="lolD"
                     /locus_tag="ACHLAG_003462"
     CDS             complement(10436..11137)
                     /gene="lolD"
                     /locus_tag="ACHLAG_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFP0656626.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(11130..12329)
                     /gene="lolC"
                     /locus_tag="ACHLAG_003463"
     CDS             complement(11130..12329)
                     /gene="lolC"
                     /locus_tag="ACHLAG_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFP0656627.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            12737..16183
                     /gene="mfd"
                     /locus_tag="ACHLAG_003464"
     CDS             12737..16183
                     /gene="mfd"
                     /locus_tag="ACHLAG_003464"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFP0656628.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            16327..17289
                     /gene="ldtC"
                     /locus_tag="ACHLAG_003465"
     CDS             16327..17289
                     /gene="ldtC"
                     /locus_tag="ACHLAG_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFP0656629.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(17372..17629)
                     /gene="bhsA"
                     /locus_tag="ACHLAG_003466"
                     /gene_synonym="ycfR"
     CDS             complement(17372..17629)
                     /gene="bhsA"
                     /locus_tag="ACHLAG_003466"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFP0656630.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         4702 a         4519 c         4452 g         4101 t
ORIGIN      
        1 accggctgga taacccgcca gccatattaa cgccatacca acggattaaa aatgccagca
       61 atggcaggaa tttgttcatc cttaaaatgg ttatgaggtt tatcaatgaa cgctgataaa
      121 gaagaaattg cactgtatta cgaagccaaa aatgacaaag tcagaaaacg ccttgggatt
      181 aaaggcggtt tttactggcg cacagcaaaa aaattatcgg ttgcaatatc acggggtgtt
      241 gtcgcaatgg acgatgctgg atttgacgaa gaggatttca aaaaacctgt tcgcgtgaat
      301 ttgcccattg ttaatgacct gccgcctgaa ggtgtgtttg ataccgaatt ctgcaaccgc
      361 tatgaaaaag gcggagaaga tggcatcaca atgatattta tagcgccttc cccctcagtt
      421 caggacaaac cagccagcac tgacaatacc aacgtcaatg gcgaagacat ggctgagatt
      481 gaggataata tgctcctgcc gatttccggt caggaactgc ccattcgctg gcttgcgcaa
      541 catggcagcg gaaaaccggt aacgcacgtt tcacgggaag aacttcaggc attacatatc
      601 gcacgagctg aagaactgcc tgctgttact gccctggcta tttcccacaa cacaaagctg
      661 ctcgacccgc tggagattcg cgaccttcac aaactggtac gcgacacaga caaagttttc
      721 cctaatcccg ttaattccag tctggggtta atgactgctt ttttcgaagc atacctgggc
      781 gctgactaca ccgatcgcgg tctgcttaca aaagagtgga tgaaaggaaa tcgtgtttca
      841 cgcatcagcc gcacgccatc cggcgctaac gctggcggcg gcattcttac cgatcgcggc
      901 gaaggttttg tccacgatga tgcgtcagtg gcgcgtgacg tagccactgg cgtactggcc
      961 cgttcaatgg acgtggatat ttataacctt catcccgcgc acgctaaacg cattgaggaa
     1021 attatcgctg aaaataaacc gccattttct ttgtttttca gcaaattcat caccatgccg
     1081 ggccacaagg attactcccg cgccatcgtg gttgcgtccg tgaaagaagc accaattggt
     1141 atagaggcta ctccccaccg tgttaccgaa tatctgaaca aagtactgac cgaaaccgac
     1201 catgccaacc ctgatccaga aatcgtggat attgcctgcg gtcgctcctc tgctccaatg
     1261 ccgcagcgtg taacaaaaga aggaaaacag gatgatgaag aaaaaccgca gccaactggc
     1321 gcaatggcag atgaacaggc aacgactgaa gcagtggaac cggatacaac tgaacataat
     1381 caggacacgc agtcgatgga tgctcagcca cagataaatt ctgttgatgc gaaatatcag
     1441 aaactgcgtg cagaactcca tgaagccagg aaaaacattc cccccaaaaa tcctgtcgat
     1501 gcagataaat tactggctgc ttctcacgga gaatttgttg aagggattag cgacccgaat
     1561 gatccgaaat gggtgaaggg gattgaaacc cgcgactctg tgaaccagaa ccagcacgaa
     1621 tcggaacaaa acgaccagaa agcggaacaa aacagcccaa atgcgttaca aaacgagcca
     1681 gaaacgaaac agcctgaacc agaagagcaa caagaaccgg aaaaagtctg caccgcctgc
     1741 ggtcagaccg gcgacggcaa ctgtcctgac tgtggcgcgg taatgggcga cgcaacatac
     1801 caggaaacat tcaacgaaga aagccaggat gaagcccggg agaaagatcc agaggaaatg
     1861 gaaagtgccg tatacccgaa caaggagtgt accgaaggcg atcaacatgc caatggcaat
     1921 aatgaaacag gcgagacaga aaatccctta attaaggtga acggtcatca cgaaagcaca
     1981 tccaccagca ggttgtggca ccatctgatg attgaccttg aaacaatggg caaaaatcct
     2041 gatgcgccaa taaacgctct ggccggtaag ttttttgatc cggcaaccgg agagatgggg
     2101 ccagaattca gcaaaactat cgatctggaa accgcgggcg gagtcatcga tcgggacacc
     2161 attaagtggt ggctgaaaca gtcacgggaa gcacaatcag ccattctgac cgatgaaatc
     2221 ccgttggatg acgcactgtt gcaattacgg gaatttatcg acgaaaactc cggtgaattt
     2281 tttgttcagg tctggggaaa tggagccaac tttgacaacg taattttacg ccgttcatat
     2341 gaacggcagg ggatcccctg cccatggcgt tacaccaatg atcgcgatgt aagaacgatg
     2401 gttgctctgg gactggtgat ggatttcgat gctcgaagtg tcatcacatt tgagggtgag
     2461 cgccataatg ccctgcacga tgcgcgttac caggcaaaat acgtttcagc tatctggcaa
     2521 aaactgatcc cgagtcaggc tgatttttaa tgttcaaccg tcgccagttg tcgttggtat
     2581 tctgcaactg gcgcgttccg gagtgatagc catgagcgaa cagtacctga taacgctcga
     2641 tgagtggaaa ccaaaacggt tcagtctccc aataacaaac actaccctgg tgaaatacgg
     2701 aaaactagga tacatcgttc caagaccaca aaaaattcgt gggcgttggc tgatagatcg
     2761 ccgagcagta tttgttgggc ctggtgaaac gggaattgcg ccggaaattc atactggcga
     2821 tgatgatgca ctgaaggaga ttttaactca tgtcaccgag gccacgaaaa aacagcactg
     2881 acgtagccgg tctttacgaa aagtttgatc gcagaactgg cagagtttac taccagtata
     2941 aaaatcctgt gactggaaaa tttcacggac tcggaacaga caaaggtaag gcagaaaaaa
     3001 tcgcttccac agccaatcag cgaatagctg cagcagaagc tgaatatttc atgcgcaaaa
     3061 ttgatgaaag tccgtcagca acaaaacgtc ggggtatcag attaaaggca tgggttgatc
     3121 gatatctgaa aatacaggat tcgcgactga aaaatggaga cattgcagct acaacccaca
     3181 aagaaaaagt gcgaatggct gcatacctgg tttcccgcct gggaaaccac ccattgaaag
     3241 aactggaggt aagagacttt gcattaatac tggacgagtg gatggataaa gacatggtca
     3301 gcacagcaag agtaaatcgc ggattatggg ttgatattta taaagaagca cagcatgcgg
     3361 gagaagttcc tcctggatgg aatcctccgg aggctacccg taaaccgatc cctaaagtga
     3421 ccagatccag actcaccctg gaagactggc aaaaaattta taatgcaacg ccagaaaaac
     3481 actttatccg taacgcaatg cttcttgcga ttgttactgg tcagcgccgt gatgacattt
     3541 gccacatgcg tttttcagat gtatggaatg gacatctaca tataacccaa ggaaaaaccg
     3601 gaatgcgtct ggccttacct cttacgctac gatgcgatga aattggggta tcgttaaaag
     3661 aagttattga tggatgcagg gacagaatgt tgagtccata cctaatccat agtaggcacc
     3721 agaaacaacc aaaaccgatg agtaaagaca acctgagcga ttacttcgcc aaagcgcgag
     3781 atctggctgg aataattcca ccagcaggaa aaacaccgcc aacatttcat gaacaacgct
     3841 ccctgtcaga acggttgtac cgcgcacagg gtgtcgatac aaaaacatta ctgggacata
     3901 aagtccaggc gaccaccgat cgctataacg atacccgagg tcaggaatgg gttaagttgg
     3961 ttatttaaca aacatcatat ggaatatccg tcaccatttc aggaaaaaag tgttgtaaaa
     4021 tgcgtgccct ggttttgggg aaaagttttg gagagatttt ggagaaagga aaaaattgaa
     4081 taaattcaaa atcctgaaag tgccccgcct tatgaataaa aaggtggaac tcgaatgatc
     4141 ggtcgactgc ttcgcggcgg ttttatgacc gctatctacg cgtacctgta tatcccaatc
     4201 attattttga ttgtgaactc ctttaacagc tcgcgctttg gcatcaactg gcagggtttt
     4261 accaccaaat ggtatagcct gctgatgaac aacgacagcc tgttacaggc agcgcagcat
     4321 tcactaacaa tggcggtgtt ttcggcgaca tttgctacgc ttatcggttc actgacggca
     4381 gttgcgctgt accgttatcg ctttcgtggt aagccgttcg ttagcggaat gctgtttgtg
     4441 gtgatgatgt caccagatat cgtgatggcg atttctctgc tggtgctgtt tatgctgctg
     4501 ggtattcagc ttggcttctg gtcgctgctg ttctcgcata tcaccttctg cctgccgttt
     4561 gtggtggtga cggtgtattc gcgcctgaaa ggttttgacg tgcggatgct ggaagcggcg
     4621 aaagatctcg gtgccagtga atttaccatt ctgcgaaaaa tcattctgcc actggcaatg
     4681 cctgcggtgg cggcgggctg ggtgttaagc tttaccctgt cgatggacga tgtggtagtt
     4741 tcttcgttcg tcaccggacc aagttatgaa attctgccgt taaaaattta ttcgatggtc
     4801 aaagtcggcg tatcgccgga agttaacgcg ctggcaacca tattactggt gctgtcgctg
     4861 gtgatggtta ttgccagcca gcttattgct cgtgataaaa cgaaaggtaa cacaggggac
     4921 gttaaatgaa aaaatggtca cgccacctgc tcgcggcggg tgctctggca ctgggcatga
     4981 gcgccgctca cgccgatgac aacaacacgc tgtatttcta caactggacc gagtacgtgc
     5041 cgccaggact gcttgaacag ttcaccaaag aaaccggtat taaggttatc tattcgactt
     5101 acgagtcgaa cgaaaccatg tacgccaagc tgaaaacgta caaagacggt gcctatgacc
     5161 tggtggttcc ttcaacctat tacgtcgata aaatgcgtaa agaagggatg atccagaaga
     5221 tcgacaagtc gaagttaagc aatttcagca acctcgatcc agacatgctc aacaagccgt
     5281 ttgacccgaa taacgactac tccattccgt atatctgggg ggcgacggcg attggcgtta
     5341 acggtgatgc ggtagatccg aaatccgtta ccagctgggc cgatctgtgg aagcctgagt
     5401 acaaaggtag cctgctgttg accgacgatg cccgtgaagt gttccagatg gcgctgcgta
     5461 agctgggcta ctccggtaac accaccgatc cgaaagagat tgaagctgca tataacgagc
     5521 tgaaaaaact gatgccaaac gtcgcagcgt ttaactccga taacccggct aacccgtaca
     5581 tggaaggcga agttaacctc ggcatgatct ggaacggttc tgctttcgtt gcacgccagg
     5641 cgggtacgcc tattgacgtg gtgtggccga aagaaggcgg cattttctgg atggacagcc
     5701 tggcgatccc ggcaaatgcc aaaaacaaag aaggcgcgct gaaattgatc aacttcctgc
     5761 tgcgcccgga tgtggcaaaa caggttgccg aaactatcgg ttatccaacg ccaaaccttg
     5821 cggcgcgtaa gctgttaagc ccggaagtgg cgaacgataa aacgctctac ccggatgctg
     5881 aaaccattaa aaatggtgaa tggcagaatg acgttggcgc agccagcagc atttatgaag
     5941 agtattatca gaagctgaaa gcaggacgtt aattcagcaa gcgggcggtg gcattaccgc
     6001 ccgtttttta tttagcaagg atgtggctaa tgagtcagga cagtaaagtg ttatcccgcg
     6061 cttttctcgg tatcggtctg atattgctcg ttatttctgc aatcatcttt tattaccatt
     6121 ttacttttac caaaagcgcg gttcatacag aaggaatcat tgttgatgcc gtttggtaca
     6181 acaatcactc aaacgacgtc gatgataatg gttcctggta tccagtggtt gcctttcgcc
     6241 ctacgccaga ttacacgcta attttcaatt cgaatatcgg tagtgatttt tatgaagata
     6301 gcgaaggtga cagagtcaat gtctattatc cgccagggca tccagaacaa gccgaaataa
     6361 acaatccctg ggttaatttt tttaaatggg gctttgttgg catagcaggc attattttcg
     6421 gtagtgtcgg tctgataatc acgctcccct ccaccaaaaa atctcgcagg aaacggaagt
     6481 cccgcccatg aaaaaattca ttatactgct cagtttactg atactcctgc ctctggtagc
     6541 aaccagtaaa ccattgattc caataatgaa gacacttttt acggatgtta ccggtactgt
     6601 accggatgct gaagagatcg cacgtaaagc cgagcttttt cgccaacaaa cgggaattgc
     6661 accgtttatt gtgattttac ccgacattaa taatgaagcc agtctcagac aaaatggtaa
     6721 agcgatgctg gcgcatgcgt catcttcatt gagtaatgta aaaggaagtg ttctgttact
     6781 atttactacc cgcgaaccac ggttaattat gatcaccaac ggccaggttg aaagtggtct
     6841 ggacgataaa catctcggcc ttctgataga aaatcacacg ctggcttatt taaatgcaga
     6901 tctctggtat cagggaatca ataatgcatt ggctgttcta caagcacaga tattaaaaca
     6961 accgacgccg ccactgacgt attatccgca tccaggacaa cagcatgaaa atgctccccc
     7021 cggcagcacc aacacattag gttttatcgc ctgggctgcg acgtttatct tattttcctg
     7081 gatcttttgc tacacgactc gttttattta tgcgttaaaa tttgcagtgg cgatgactat
     7141 tgccaatatg ggttatcagg ctttatgttt gtatatcgat aatagctttg cgattaccag
     7201 aatatcgcca ctctgggcag ggctaattgg tgtctgtacg tttattgccg cattgttatt
     7261 gacgagtaaa cgataaaaga ggccggaaaa gtgcgcgtgt attatgccgg atggggcgcg
     7321 aacgccttat ccggcccaca aaacccgcaa attcaattaa ttgcgtcccc ttgtaggcct
     7381 gataagcgta gcgcatcagg caatgcttcc tgctttataa tcccttcagc aacttttcaa
     7441 caaactcagg caccacctgg cttgccgggc cgtaatattt ctcggcaaat tcattaccga
     7501 cctgacttgg ttcgagattc agttccacgg tgtgcgcgcc atgcagtttc gcttcgtgaa
     7561 caaacccagc cgccggataa acatgcccgg aagtaccaat ggcaatgaaa atatcagcca
     7621 tcgacaacgc catataaatt tcatccatgc cgagtggcat ttcgccaaac caaacgacgt
     7681 gcgggcgcaa gggtgccggg aactggcaac aatggcattt atcttctggc gtaacgtctc
     7741 cggtccagtc gagaacctga ccactttgcg aacaacgaac tttcagcaat tccccatgca
     7801 tatgaatgac attggtatta cctgcgcgtt catgcaggtt gtctatattc tgcgttacca
     7861 gcaaaaagcg atcgccgagg gcgtcttgca gtttagccag cgcaagatgc gcggcgttag
     7921 gctgaatttc tggctgctgc agctgtcgac gacgggcgtt ataaaacgct tgcaccagtt
     7981 caggatcgcg atcgaaacct tccggcgttg ccacatcttc aacccgatgt tcttcccaca
     8041 ggccatctgc ggcgcgaaag gtacgaatac ctgattccgc agaaatccct gcccctgtca
     8101 gtacgagtac tcttggtttt tccattgctt ccggcaccac tttatctctg aaaaaaatac
     8161 gctgacgcaa acgctcgcgc aggcggcgtt tatttttacg aaaacgactt aaccgatgac
     8221 cccgacgcga cagcatagca acctctgttg tttaatcggt tagatgtagg aaggccgcac
     8281 cacgcattcc tcccgcatca ccgtggcgcg cgcgttcaat gcgcggaaca cgagctacag
     8341 gtaagagatg acgaggcagc ctgtccgcca gttgcgttgt gattgccggg aaattcgata
     8401 agccaccacc aatgacgacc aggtcagggt caacaatggt caggatattt cccagacaaa
     8461 ccgctaataa atccagataa cgctcaacgt gcgcccttgc ctgctcatcg ccttgatcat
     8521 aaagcgcaat aatttcggga gcctgcaacg gttgatgata atagtgttga tacagccacg
     8581 caaaaccgcg accagacaga taattttcaa tgcagccatg ctgaccacag ccgcagcggc
     8641 gtaacgggaa atccaacccc atcatggtta acgcatcaac cggcagacgc atatggccaa
     8701 actcgccggt aatgtagctt ttaccggtaa tcggtttgcc gttgaaaatc agcccgccgc
     8761 caacgccggt gccgagaatc aaccccatca ccagtggata ttgagtaaat tcatcatccc
     8821 aggcttctga aagggcaaaa cagttggcat cgttatcaag gcgtacatcg cgatcaagac
     8881 gtgcgctcag gtcggcacgc agcggtttac cgctggcagc ggggacattg gcggcataca
     8941 gcgtaccatc ttctgtttcc ggcattcccg gaataccgat gccgacagaa cctttacagc
     9001 caaaacgttg gtcagcttca gctaccagct cacacactgc atctaaaaat gcgtcatagc
     9061 tgtcacgcgg tgtcggcacc cgcttttccc actgcaactg ccgaccgcta tcaaacacgc
     9121 caagcgcaat ttttgttcca ccaatatcaa acccgtaata cattgccgct ccttgtttta
     9181 atgtactgcc tttactggcc gctaaggact cgcgcagggt caatattact ggcgcgccgc
     9241 gccggatacc aacttgccaa aagactcagc aacaatgctg tgaccagtac gtagaagacg
     9301 tccagccagt gcaattccga tggcaggaaa tcaataaaat agatatcgct ggagaggaac
     9361 tgatgaccga tcaacttttc aatccactca ataatcgggg taagttgcag tgaaaccacc
     9421 acaccgataa tcaccccaca caggctgccg aacagccctg ccagcaatcc ataccagaca
     9481 aagatggcgc gaattaaacc atctttcgcc cccagcgttc ttaatactgc gatatcgcca
     9541 ctcttgtctt tcaccgccat cactaaggtg gagacgatgt tgaaacaggc cacgccaatc
     9601 accagtacca tcgccagata cataatggcg cggatcatct ggatatcgcg atacatatag
     9661 ccgtaagtac caatccaact tttaatataa acatagctgt tggtcacttc acccgcatcg
     9721 cgtaccagct tattggcgtt gaaaacatcc gtcattttaa gggcaatacc tgacacgctg
     9781 gaacccatat caagatattg ttgggcatcc gccagcggga tcatggcaaa actgtgatcg
     9841 agttgaccac tcaactgcaa aataccggca acgtgcaaac gcacacgttt tggctgcatc
     9901 agtttatgct cgggattcga gttggggatc ataatcgaca cccaatcccc ctgcttcact
     9961 ttcagcgcat ccgccacgcc tttgccgatg ataatttgct gttcgcccgc tttaaaattg
    10021 cgccacgcat cgccctggac aaacgagggt aatgcgctca gacgctgttc ctgttgcggg
    10081 ttaacgccct tcacctggat tgcgcgaaga ttcacgccac tttccaccag cccggtgaaa
    10141 ttgatatacg gcgcggcagc ggcaataccc ggcacttttt gcacgtgatc cagtgcttcc
    10201 tgccagttag tccacggctg atccaccgcc tcgatttcac catgcggcac caccgccaga
    10261 atacggttgt tcagttcgcg ttcaaagccg ttcatcgcgc ttaagccgac gatcaatacc
    10321 gccacgccga gggcaatgcc aatggtagaa ataacggaga tcagcgacac catgccgccg
    10381 cgccgccgtc cgcgactaaa acgcaggcca attaataacg ataaaggcat cgccattact
    10441 ccgcccccat caggctcagt tccgccgtca gacgaccatc acgcatttct aactggcggc
    10501 tcatacgttt cgccagttgc aggtcgtgag taaccaccag gaaggcggtg ccctgcaagc
    10561 gattcaattc cccaagcaac tggaagatgc tgtcggcgtt acgcgcatcg aggttaccgg
    10621 taggttcatc cgccagtacc aggcgcgggt tattcaccag cgcacgggca atcgccacac
    10681 gctggcgttc gccgccagaa agttcagatg ggcggtgatt cgcacgatga tccagcccca
    10741 ccgcttttaa catctcaagt gcacggctgt tgatttcagc gggctttttc ttgccaatca
    10801 gcagcggcat agccacgttt tccagggcgg taaaatccgg cagcaggtgg tgaaactgat
    10861 aaataaagcc cagcttctgg ttgcgcagct ctgctttcgc cgccgaagac agtttgctca
    10921 tcggttgacc gttaaagatc acatcgccgg aggttggcgt atccagcccg cccaacaagt
    10981 gcagcaaggt gcttttaccg gaaccagagc taccgacgat cgccatcatt tcaccttcgc
    11041 cgacgctgaa actgacattg tgcagcacat cggtttgcac actgccttcc tgatagcgtt
    11101 tgcacaggtt gtcgcattgc aacaggatct tattcataac gtaaagcctc agcgggttga
    11161 gtggcggcag cgcgccatga agggtaaagc gtagacagca gcgcgatagc catcgccacc
    11221 agcgcaataa caatgacctg taaaggttcg atagcgaccg gcagcgccgc gccatcaagc
    11281 aggacgccga ttatcggcat cagattattt aactggctgg caagcagggc gccaagcgcc
    11341 gctccgagga tcgcaccgat aatcccggcg ctggcccctt gcaccataaa gaccatcatg
    11401 atttgtcgcg gagttaagcc ttgcgtttgc aggatcgcta cttcgccctg cttctccatt
    11461 accatcagcc ctagtgaggt aataatgtta aacgccgcaa cggcgacaat caggctcagc
    11521 agtaaaccca tcatattttt ttccatgcgt acggcctgga acaactcgcc tttacgatca
    11581 cgccagtcct gccatttgct gccttcaggc agtttttgct gacttaatga gtcgactttc
    11641 agcggctcat ccagccacaa acgccagccg gtaatattgc ctgccggata acgcatcaga
    11701 cgcgaggcat cctcaatatt caccagcatt tcattgccat cgacttcact gttggcggcg
    11761 aaagtgccaa tcacattgaa caggcgctga cttggaatac gccccatcgg cgtgaactgg
    11821 ctggcagatg gtaccatcat gcggatttga tcaccgcgat taacgcctag ctgtgaggca
    11881 agttgttcgc cgaggatgac attatatttc cccggctcga ggtcagtttg tttcacattg
    11941 accagatacg gcgttagtgg atctttttgc gccgggtcga taccgagcat caccccgacc
    12001 gccacgctgc gcgcgctttg cagtaccaca tcaccggtag taataggtgc gacgcgatta
    12061 acgccgtcca gtttgactgc cgtttccggg agttgctgcg ggttaagaga gccatgctca
    12121 gaagagagaa ttgcctgtgg catcaggcca aggatgttgt tttgcagctc gcgctcaaag
    12181 ccgttcatca ctgacaatac tgtgaccagc gccatcaccc cgagggtaat gccgatggta
    12241 gaaagccagg agacgaaacg accgaagcga tccgctgcac gcccacgcat gtaacgcagg
    12301 ccaataaata gagcgacagg ttggtacatg aaatccgtct ggttgctgta gcaaagacac
    12361 gagtatataa gcgaaagcaa gatgggtaaa cgtctgaact gtaagtgatc gtcagggcta
    12421 atctgaaaag cccccgtgaa tcaatcggct aaattgccat gttttatcat caatcttgcc
    12481 gcctgatctt aatctgaaaa atcatccaat acagacagat acccattaca catagggtca
    12541 taacccgcag gatcaaccgc atgtaaatta aaaaagagcg tgttgtcctg atgaaacaaa
    12601 aagagctatg gattaaccag atcaaagggt tatgtatttg tcaaaccatg tcgattacgg
    12661 ataatgaaca gcattgctta tcagagatat gcccccatat gttgaggcat atcctaacga
    12721 gaatctgaca accgttatgc ctgaacaata tcgttatacg ctgcccgtca aagcgggtga
    12781 gcagcgtctg ctgggcgagt taaccggcgc tgcctgcgcg acgctggtag cggaaattgc
    12841 cgaacgtcac gccggtccgg tggtactcat tgcaccagat atgcaaaatg ctctgcgttt
    12901 gcatgatgaa atcagccagt ttaccgatca aatggtgatg aatctggcgg actgggaaac
    12961 tcttccctac gacagttttt cgcctcatca ggacattatc tcctcgcgcc tttccaccct
    13021 ttaccagcta ccgacgatgc agcgtggcgt actgattgtt ccggtgaata cgcttatgca
    13081 gcgcgtttgc ccgcacagtt ttcttcacgg tcatgcgctg gtgatgaaaa aaggtcagcg
    13141 tctgtcacga gatgcattaa gaacccaact ggacagcgcc ggttatcgcc atgttgacca
    13201 ggtaatggag cacggcgaat acgccacgcg cggcgcgttg ctggatctct tcccgatggg
    13261 gagtgagctg ccttatcgtc ttgatttctt tgatgatgaa atcgacagcc tgcgggtgtt
    13321 tgacgtcgac agccagcgca cgctggagga agtagaagcg atcaatctgc tgcccgcgca
    13381 cgaatttccg accgataaag cggcaattga actgttccgc agccagtggc gcgatacctt
    13441 cgaagtgaag cgcgatccgg aacatattta ccagcaagtg agtaaaggca cattacctgc
    13501 cgggatcgag tactggcagc cattgttctt cagcgaacca ctgccgccgc tgttcagtta
    13561 tttccctgcc aataccttgc tggtgaatac tggcgatctg gaaaccagtg ccgaacgttt
    13621 ccaggctgac acgctggcgc gttttgagaa tcgcggcgtc gatccgatgc gcccgctgtt
    13681 gccaccacaa tcgctctggc tgcgggtgga cgagctcttc tcagagctga aaaactggcc
    13741 ccgggtgcag ctaaaaactg aacatttacc gacaaaagcc gcgaatgcca atttaggttt
    13801 ccagaaactg ccagacctgg ccgttcaggc acaacaaaaa gcgccgctgg atgcgctgcg
    13861 taagttcctc gagactttcg acggtccggt ggtgttctcg gtagaaagtg aaggtcgccg
    13921 tgaagcgctg ggtgaactgc tcgcacgaat taaaattgct ccgcaacgca ttatgcgtct
    13981 tgatgaagcc agcgaccgtg ggcgttatct gatgattggc gctgccgaac atggttttgt
    14041 cgataaggtg cgtaatctgg cgctgatctg cgaaagcgat ctgctcggtg aacgtgttgc
    14101 ccgccgtcgt caggattctc gccgcaccat caaccccgat acactgatcc gtaaccttgc
    14161 ggagctgcat attggtcagc cggtggtcca tctggagcac ggtgtcgggc gctatgccgg
    14221 aatgaccacg ctcgaagcgg gcggcattac tggcgagtat ttgatgctca cctatgccaa
    14281 cgacgccaaa ctgtatgttc cggtgtcgtc actgcatctg attagccgtt acgcaggtgg
    14341 cgcggaagaa aacgccccgc tgcataaact tggcggcgat gcgtggtcac gcgcgcggca
    14401 gaaagcggcg gaaaaagtgc gtgatgtggc ggcggaattg ctggatatct acgcgcaacg
    14461 cgccgccaaa gagggcttcg cgtttaaaca cgatcgtgag cagtatcagt tgttctgcga
    14521 cagcttcccg tttgaaacca cgccggatca ggcgcaggcc attaatgcgg tacttagcga
    14581 catgtgtcag ccgctggcaa tggatcgtct ggtgtgcggc gatgttggct ttggtaaaac
    14641 agaagtggcg atgcgcgccg ctttcctggc agtagataac cacaagcagg tggcggtgtt
    14701 ggtgcctacc acccttctcg cgcagcagca ttacgacaac ttccgcgacc gtttcgccaa
    14761 ctggccggta cgtatcgaaa tgatctcccg tttccgcagc gccaaagagc agacgcaaat
    14821 ccttgcggaa gtggcggaag ggaaaatcga tattctgatc ggtacgcaca aactgctgca
    14881 aagtgacgtc aagtttaaag atttaggcct gctgattgtc gatgaagaac accgcttcgg
    14941 ggtgcgtcat aaagagcgta ttaaagcgat gcgcgcgaac gtggatattc tgacgcttac
    15001 tgcaacgccg atcccacgta cgctgaatat ggcaatgagc ggaatgcgtg acctgtcgat
    15061 tatcgccacg ccgcccgccc gtcgtctggc agttaaaacc tttgtccgtg agtatgacag
    15121 cctggtggtc cgggaggcga tcctgcgtga aattttgcgc ggaggacagg tttattatct
    15181 ctacaatgat gtggaaaaca ttcagaaagc cgccgaacgg ctggcagaac tggtgccaga
    15241 agcgcggatc gccatcggtc acgggcagat gcgcgagcgc gaactggaac gggtgatgaa
    15301 tgatttccat catcaacgtt tcaacgtgct ggtttgtaca accattatcg aaaccgggat
    15361 cgacatcccg acagccaaca ccattatcat tgaacgcgcg gatcacttcg gtctggcgca
    15421 gctgcaccag ttacgcggtc gcgtcggacg ttcacatcat caggcatatg catggttgct
    15481 gacgccgcat ccaaaagcga tgactaccga tgcacaaaaa cgtcttgaag cgattgcctc
    15541 gctggaagat ctcggggcag gttttgcgct ggcaacgcac gatctggaga ttcgcggcgc
    15601 gggtgaactg cttggcgaag aacaaagcgg ctcaatggaa accatcggtt tctcgctgta
    15661 tatggagttg ctggaaaacg ccgtcgatgc actgaaagcc ggacgcgagc cgtcgctgga
    15721 agatctcacc agccagcaaa cagaagtcga gctgcggatg ccgtcgctat tgccagatga
    15781 tttcattcct gacgtgaata cgcgtttgtc gttctataaa cgtattgcca gcgccaaaac
    15841 ggaaaacgaa ctggaagaga tcaaagtcga gcttatcgat cgcttcggcc tgctgccgga
    15901 tccggcgcgt accctgctgg atgttgcccg tctgcgccag caagcgcaga aactggggat
    15961 caggaagctg gaaggtaatg agaaaggcgg ggtgatcgaa tttgccgaga agaatcacgt
    16021 taatccggcc tggttgattg gtttgctgca aaaacagccg cagcattacc gccttgatgg
    16081 tccgacgcgc ctgaaattta ttcaggattt gagtgagcgg aaaacgcgta tcgaatgggt
    16141 acgccagttt atgcgtgaac tggaagagaa cgcgatcgct taatggtaaa tcgggggcgt
    16201 ttctgcgccc ccatctgcaa catttacaaa ttctttgcac ttccctgcac tatccgacac
    16261 tgtcaccatc cataattcag gcttatctgt ttattacaat aaccttatat ttattatgga
    16321 tttttggtga tgatcaaaac gcgtttttct cgctggctaa cgttttttac gttcgccgct
    16381 gccgtggcgc tggcgctacc ggcaaaagcc aacacctggc cgctgccgcc agcgggcagt
    16441 cgtctggttg gcgaaaacaa atttcatgtg gtggaaaatg acggtggttc tctggaagcc
    16501 atcgccaaaa aatataacgt cggctttctc gctctgttac aggctaaccc tggcgttgat
    16561 ccttacgtac cgcgcgcggg tagcgtgtta acgatcccgt tgcaaaccct acttccagat
    16621 gcgccgcgcg aaggcattgt gatcaatatt gcggagctgc gtctctatta ctacccgccg
    16681 ggtaaaaatt cggtaaccgt gtatccaata ggtattggtc agttaggtgg tgacacgctg
    16741 acaccgacaa tggtgaccac cgtttcagac aaacgtgcaa acccaacctg gacgccaacg
    16801 gcaaacatcc gcgcccgtta taaagcacag ggaattgagt tgcctgcggt agtgccggct
    16861 ggaccggata acccaatggg ccatcatgcg attcgtctgg cggcctatgg cggcgtttat
    16921 ttgcttcacg gtacgaacgc cgatttcggc attggcatgc gggtaagttc tggctgtatt
    16981 cgtctgcggg atgacgatat caaaacactc tttagccagg tcaccccagg caccaaagtg
    17041 aatatcatca acactccgat aaaagtctct gccgaaccaa acggtgcgcg tctggttgaa
    17101 gtacatcagc cgctgtcaga gaagattgat gacgatccgc agctgctgcc aattacgctg
    17161 aatagcgcaa tgcaatcatt taaagatgca gcacaaactg acgctgaagt gatgcaacat
    17221 gtgatggatg tccgttccgg gatgccggtg gatgtccgcc gtcatcaagt gagcccacaa
    17281 actctgtaaa tcctggataa aaaaaatccc cgcgacacgg catgtcgcgg ggaaaaaatc
    17341 agcagtagca ggcattaatg agggttaatg cttatttata aattactgct gttccatgga
    17401 gggtattcgg accggttaca gaagtaatac ggaaagattt tgcgcccatc tcatccgctt
    17461 tttgcgccag ctgctcttcc agcgatccca gatttgtccc cgcgttagca ctgattgtac
    17521 cgactttttg ttggccttct ggcgttgact gaacttcgac agccgcaaag ctggcaaatg
    17581 acatggagct taaaatcgcc gcagcgatga gggtttttac gtttttcata atagtggcct
    17641 tatgcagatg aatgacgtcg caagcaatta cttaacgatc gataactaaa tgatagatgt
    17701 gatctggatc acatacaaga tattttttat aacaatcatt aattaattta aaaatcttta
    17761 aatttcagtt aaat
//
LOCUS       DAZGVQ010000108        17669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000108 DAZGVQ010000000
VERSION     DAZGVQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.109"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            70..573
                     /locus_tag="ACHLAG_003467"
     CDS             70..573
                     /locus_tag="ACHLAG_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFP0656631.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(640..2097)
                     /gene="nuoN"
                     /locus_tag="ACHLAG_003468"
     CDS             complement(640..2097)
                     /gene="nuoN"
                     /locus_tag="ACHLAG_003468"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFP0656632.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2104..3633)
                     /gene="nuoM"
                     /locus_tag="ACHLAG_003469"
     CDS             complement(2104..3633)
                     /gene="nuoM"
                     /locus_tag="ACHLAG_003469"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFP0656633.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3797..5638)
                     /gene="nuoL"
                     /locus_tag="ACHLAG_003470"
     CDS             complement(3797..5638)
                     /gene="nuoL"
                     /locus_tag="ACHLAG_003470"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFP0656634.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5635..5937)
                     /gene="nuoK"
                     /locus_tag="ACHLAG_003471"
     CDS             complement(5635..5937)
                     /gene="nuoK"
                     /locus_tag="ACHLAG_003471"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFP0656635.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5934..6488)
                     /gene="nuoJ"
                     /locus_tag="ACHLAG_003472"
     CDS             complement(5934..6488)
                     /gene="nuoJ"
                     /locus_tag="ACHLAG_003472"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFP0656636.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6500..7042)
                     /gene="nuoI"
                     /locus_tag="ACHLAG_003473"
     CDS             complement(6500..7042)
                     /gene="nuoI"
                     /locus_tag="ACHLAG_003473"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFP0656637.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7057..8034)
                     /gene="nuoH"
                     /locus_tag="ACHLAG_003474"
     CDS             complement(7057..8034)
                     /gene="nuoH"
                     /locus_tag="ACHLAG_003474"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFP0656638.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8031..10757)
                     /gene="nuoG"
                     /locus_tag="ACHLAG_003475"
     CDS             complement(8031..10757)
                     /gene="nuoG"
                     /locus_tag="ACHLAG_003475"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFP0656639.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10810..12147)
                     /gene="nuoF"
                     /locus_tag="ACHLAG_003476"
     CDS             complement(10810..12147)
                     /gene="nuoF"
                     /locus_tag="ACHLAG_003476"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFP0656640.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12144..12644)
                     /gene="nuoE"
                     /locus_tag="ACHLAG_003477"
     CDS             complement(12144..12644)
                     /gene="nuoE"
                     /locus_tag="ACHLAG_003477"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFP0656641.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12647..14449)
                     /gene="nuoC"
                     /locus_tag="ACHLAG_003478"
     CDS             complement(12647..14449)
                     /gene="nuoC"
                     /locus_tag="ACHLAG_003478"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFP0656642.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14543..15205)
                     /gene="nuoB"
                     /locus_tag="ACHLAG_003479"
     CDS             complement(14543..15205)
                     /gene="nuoB"
                     /locus_tag="ACHLAG_003479"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFP0656643.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15221..15664)
                     /gene="nuoA"
                     /locus_tag="ACHLAG_003480"
     CDS             complement(15221..15664)
                     /gene="nuoA"
                     /locus_tag="ACHLAG_003480"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFP0656644.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16295..17233)
                     /gene="lrhA"
                     /locus_tag="ACHLAG_003481"
     CDS             complement(16295..17233)
                     /gene="lrhA"
                     /locus_tag="ACHLAG_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFP0656645.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPLPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4388 a         4949 c         4593 g         3739 t
ORIGIN      
        1 acatgcacat tttattttcc ctcgtctgtt ttaagatgcg aaacatgaaa ttcaatggag
       61 gaaaaataaa tgggaatgat tggctatttt gcagaaattg actcagaaaa gattaaccaa
      121 ctgcttgagt ctactgaaaa acccttaatg gacaatattc atgacacgct ttcaggatta
      181 cgccgactgg atattgacaa acgctgggat tttttacact ttgggctgac aggcacctct
      241 gcttttgatc ccgcgaagaa tgatccgctg agtcgtgccg tgcttggtga acacagcctc
      301 gaagatggca tagatggctt cctgggattg acctggaatc aggagttagc cgcgacaatc
      361 gaccgactgg aatcactgga tcgaagtgag ctgcgtaaac agttttccat taagcgactc
      421 aatgaaatgg agatttatcc gggagtgacc ttttcagaag agttagaggg gcaactcttt
      481 gcctcgatca tgcttgatat ggaaaagctg attagcgcgt atcgacgtat gctgcgacag
      541 gggaatcatg cgctgacggt tattgttggg tgatggtgtc agggcaccaa aaaaaaccgc
      601 cgaacatgtc ggcggctttc tgacttacaa actgatagct tacatcagcg gcattgctaa
      661 ccgcacaatg ctaatcagcg gttgtggcca tacacccagc accagtacca acagtgcaga
      721 aatcagcacc acgataccgc ccgcgctgta ctgccagttt gacggtgcat cgcgacccgg
      781 ttgttccggt gcgtgcaggt acaggctcac cgccacgcgc aggtagtagt agaggccgat
      841 tgccgaaccg acaaccacag cacccaccag ccaccacaag tgtgcctgga caccgaccgc
      901 cagcacgtag aacttaccga taaagcccag cgtcatcggg ataccggcca gagacagcat
      961 catcaccgtc atcaccgccg cgaggatcgg gcgatgccag aacagaccgc ggtaagagaa
     1021 cagggaatca gcatccgggc cacggtacgg gctggacatc aggctgacca cgccgaacgc
     1081 gccgaggctg ctgaacagat aaccggccag gtaaactcct accgcttcca tcgacatctc
     1141 gcccgtttgc agcgcaatca gcgccaccag cagatagccg aggtgagaga tagatgagta
     1201 accgagcaga cgtttgatat tggtctggct cagcgccatc aggttaccga agatgataga
     1261 ggcaaaggcg ataatcgcca gcactacccg aatcgcttcg ctgtcaccca ccggtgcgta
     1321 gaggaacaga cgcatcacca caccgaagat agcgattttg ctcgccgtcg ccaggaaagt
     1381 ggaaaccggc gcaggcgcgc cctggtatac gtctggcgtc cacaggtgga acggcaccag
     1441 agagagtttg aagccaaggc caacaatcat caggccgaaa cctgccagca acagcggctc
     1501 gttgagcata ccgtcgccga ggtttttacc cagcgcgaca aacgacaggt cgccagactg
     1561 cgcatacacc agcgccatac cgaacagcag gaaagaagac gctgcggcag aaaggatagt
     1621 gtatttgata ctggcttcca gcgaacgttt ctggcggaaa gcgtaaccga ccaggccaaa
     1681 cagcggcaaa gagatcagtt cgataccgag gaacagagac gccagatggt tggcattcgc
     1741 cagcaggatc ccgcccagcg cggcaattaa caccaacagg tagaactcat ccttgttgtc
     1801 gttatagcct tcaagccacg ggtaggcgaa agtacaggtg gcgaggctcg ccaacaatac
     1861 cagcccggtg taaagcatgg cgaaaccatc aacgcgcatg agcggcgtaa cgtccatagc
     1921 gcccgcctgg ccaacaaacc agagcgaaac cagcgccgcg ttaagcccaa taaccgagag
     1981 cgtagcgttg aggaaatgat tgcgtcgcca cgcaatggag agcatcacaa ccaccaccgt
     2041 caagccgacg atcagcaacg gtagcagtgc gatcaggttt tgtggagtta ttgtcatggc
     2101 gatttacggc cttgtagtag taacggaatt aacaaaccac tgctggatat tgccaatcgc
     2161 ggagtgcgag gtatccagaa tcggctgcgg atagaagccc agcagtacca gcagcaccac
     2221 cagcaacagg atcataaaca gctcacgcag cgacatccct ggcagttcct ggctggcaat
     2281 ctggcttttc gctttaccga agtaagcgcg atgcaacatc gccagcgaat aaacagatgc
     2341 aaagaccagc ccaaaggtag agataacggt aatcaccggg acaacctgga agctgccgaa
     2401 cagaatcata aattcgccga cgaagttacc ggtgccaggc atcccaagcg ttgccaccgc
     2461 aaagaacagc gacagtgctg gcagccattt catcttgctc cacagaccgc ccatcatgcg
     2521 catgtcgcgg gtatggatac gttcataaag ctgaccacaa agaataaaca gacccgccgc
     2581 cgacaaaccg tgcgcaatca tctggattac cgcgccctgg taggccaact ggctgccggt
     2641 gtagatagca atcagcacga agcccatgtg ggaaaccgag gtgtaggcga tcagacgttt
     2701 gatatcggtc tgggcgaagg ccatccacgc accgtagaag atgccgataa cacccagcca
     2761 catagcaatt ggcgcgaact ctgccgacgc gttcgggaac agcggcaggg agaaacgcag
     2821 caaaccgtaa gcggcagttt tcagcaagat ccccgcgagg tcaacggaac cggcggtcgg
     2881 agcctgggag tgcgcatccg gcagccagcc atgcagcgga accaccggca ttttgactgc
     2941 gaaggcgatg aagaagccca gcattaacag gtattccaca ccactggaca ttggcgtatt
     3001 cagcagctct tcatagttga aggtccagac gccggtcgca ttgtagtgaa caaaaaccag
     3061 cgccaggatg gcaatcaaca tcaccaggcc actcgcctgg gtgtaaatga agaacttggt
     3121 tgccgccgtg atacgcgttt taccgtcaga ggctttatgc ccccacagtg cgatcaggaa
     3181 gtacatcggc accagcatca tttcccagaa gaagaagaac aggaacatgt cgatggcaag
     3241 gaacacgccg ataacgccgc ccaggatcca catcaggttg aggtggaaga agccctgata
     3301 tttttcgatc tctttccacg aacatagtac cgccagcaca ccgagcagac cggtcagcac
     3361 gaccatcagc agcgacagac cgtcaatggc gagatgaata gagataccaa aacgcgggat
     3421 ccacggcatg tcgaattcag actgccactg cggaattccg gcggattgcg tcagtgaata
     3481 accgccctgc aaccacagtt gcagcgacag cgccagcgtc aatcccatgg tgatcagcgc
     3541 gatccagcgc ggcaccttga cgccaaagcg ttcggtctgc cagcacagga agccgccaat
     3601 aaagggaatt aatattagcc agggtagtaa catggcgatc tttattcctt gtaaaagtcc
     3661 cgtcaggacc ggattttcaa cgaattctca cgacagaaaa ttctcttcgg actgggggta
     3721 gtgtcggatg cgccgcgaag gcagcttatc cgacctacgt tcttatatgc ccaggggcaa
     3781 tcccacaatc cttaactcaa cgcagtacca tcaacagtgc cagcaccacg accgcaccga
     3841 tgctcatgga tgccacatac cagcgcagat aaccgttctc gcttaacagc agacctttac
     3901 ctgcaaagcg ggaaagaacc gccgggatgt tcatcattga gttcagcgga tcgcgtttca
     3961 gcaaccaggc aatacccagg aacggcttga cgaacacttt gtcgtacagc cagtcaaagc
     4021 cccaggcgtt gtaccaccag gtactcagca ggcggcccgg cgcactgttg gcgatggagg
     4081 tcaccagagt acgtttaccc agccacagcc aggctgccag cagaatgccg accaccgcga
     4141 ccacgccaga ggtaatttcc agggtcaaca tgctgccgtg cgccagttcc gtcgtttgcg
     4201 gaagcacgcc ctgcagcggc ggtacaatca gtgcgccaac gaaggtggaa aggatcagca
     4261 gcacaatcag cggcaggctg tgagttaccc ctttcacggc gtgagcgtga atttgttctt
     4321 ttccgtggaa gacgatgaaa atcatacgga aggtgtagag cgaggtcata aacgcaccga
     4381 ccagacctgc caccatcaga ttgatatgac cattcgccat cgcacccgcg aggatctcat
     4441 ccttactgaa gaagcccgca gtgaccagcg gtagtgccga cagtgctgcg ccgcccacca
     4501 ggaagcagag ataaaccagc ggaatagatt tacgcagacc gcccatcttg aagatgttct
     4561 gttcgtgatg gcaggccaga atgacggaac cggatgccag gaacagcagc gctttaaaga
     4621 acgcgtgagt catcaagtgg aaaatcgccg catcccatgc ctgcacgcca agcgcgagga
     4681 acatgtagcc aatctggctc atggtagagt aagcgagaac acgtttgatg tcggtctgta
     4741 ccagcgcggc aaaaccggcc agcagcagcg taaccgcccc gacaataccc accagatgca
     4801 gaacttccgg cgtcatcagg aacaggccgt gggtacgggc gatcaggtag acacccgcgg
     4861 ttaccatcgt tgcggcgtgg atcagcgcgg agacaggcgt cgggcccgcc atcgcgtcag
     4921 caagccatgt ctgcaacggc aactgcgcag atttaccgac cgcaccgccc agcagcatca
     4981 gcgtcgccca catcagcatg ttattgccgt cagcaaagtg cgctggtgcc agttccacca
     5041 tttcgcggaa gttcagggtg cccagttcgt tgtaaagaat gaacagtgcg aaagcgagga
     5101 aaacgtcacc cacacgggtc acgacgaacg ctttcattgc cgctgcgcca ttcttcggat
     5161 cggtgtaata gaaaccgatc agcagatagg agcacaggcc cacgccttcc cagccgaggt
     5221 acatcagcag caggttatcg gcgagaacca gaactaccat gctggcgatg aacaggttgg
     5281 tgtaggcgaa gaagcgagag tagccctctt caccgcgcat gtaccaggag gcgtacatgt
     5341 gaataaggaa acccacacca gtgaccaccg agagcatggt cagcgacagg ccgtccagca
     5401 ccaggttaaa accgatgtta aagtcgccta ccgacatcca cgtccacagc ggctggctgt
     5461 atgcctgctc gccgttagcg aagaaatcaa cgccgataaa ggcggttacc agcgccgcca
     5521 ggcccacaga gccaacgccg acgatcgccg aaacgttttc agaccagcgc ccacgggaga
     5581 acgccagcag gacgaagcca atcaatggca aaataatggt taaggcaagc atgttcatcc
     5641 gcgcatctca cttactgaat cgatgttcag gttctggcga cgacggtgaa gttgcagcag
     5701 cagcgcaagg ccgatactcg cttctgccgc cgcgaggctg atggcgagaa tgtacatcac
     5761 ctgaccgtcg gtctgacccc agtagcttcc ggcgaccaca aaggccagcg cggaggcgtt
     5821 aatcatgatt tccagaccaa tcagcataaa cagcagattg cgacggataa ccagaccggt
     5881 taagccaaga acgaataaga ttgccgcgag gatcagtcca tgttgtaagg ggatcatgcg
     5941 tgctcctccg tttttctttt cgcgctgtcg tctttacgat tgctcagcac ttcacccgca
     6001 cgctcttcac gaccgacgtg gaaggccaca accagacccg cgagcagcag catagaagcc
     6061 agttccaccg ccagtacgta aggcccgaac agcgtaatac ccactgcttt agcactgatt
     6121 ggcgtaccgt cgataccctg atcattaaca ccaaggatgg cgtaaacaat caccaccagc
     6181 atgatggccg acaaaattgc cggaccaatc cacacctgcg gtttcagcca ctggcgttcc
     6241 tgttcgattt ctgaaccgcc caggttgagc atcatcacca cgaacacgaa cagcaccata
     6301 atggcacccg cgtagacgat aatttccagc gcaccggcga agtaagcgcc cagtgagaag
     6361 aacaccccgg aaatcgccag cagcgaaata atcaggtaca gcagtgcgtg taccggattg
     6421 gtatgggtga tcactcgcaa ggtcgcaagt atggctatca ggccacagat ataaaaagcg
     6481 aactccattg cccctctcct tacggtaaca ggctcttgac gtcgataggc ttggcttcgt
     6541 tctctgcttc gcccttatct ttgccgtcga ttgccatacc tgccatccgg tagaagttat
     6601 attccgggta tttgcccgga ccggagatca gcagatcctc tttctcgtaa accagatcct
     6661 ggcgcttgta ttcccccatt tcgaaatccg gggttaactg aatcgccgtg gtcggacagg
     6721 cttcttcgca cagaccacag aaaatgcagc gtgagaagtt gatgcggaaa aattccgggt
     6781 accagcgacc gtctttggtt tctgcttttt gcagcgagat acagccgacc gggcaggcta
     6841 ccgcgcagag gttacaggct acgcaacgct cttcgccgtc cgggtcgcgg gtcagaacga
     6901 tacgaccacg ataacggggc ggcagataga ccggctcttc cgggtacatt cgcgtttcgc
     6961 gtttggcgaa cgcgtgcagg ccgatcatcc agatactacg aacctgggtg ccgaaaccta
     7021 ctaacaattc ttttaaggtc atggtcttat tgccccttat tgcgcctgcc agagaatgac
     7081 agccgccgtt accagcaagt tgatcagcgt cagcggcagg cagattttcc agccgaagga
     7141 cattacctgg tcataacgcg gacgcggtaa cgacgcacga atcaaaatga acatcatcat
     7201 aaagaacgcg gttttcagcg cgaaccagat gaatggcggt aacaacgggc cttgccagcc
     7261 accgaagaac agcgtcacca tcaatgcaga gatggtcaca atcccgatgt actcacccac
     7321 gaagaacaga ccgaacttca taccggaata ttcaatgtgg taaccatccg ccagttcctg
     7381 ctcggcttcc ggctggtcaa acgggtgacg gtgacatacc gccacgcccg cgatggcaaa
     7441 ggtaataaaa ccaaagaatt gcgggataac gttccacaca tgcgcctggc tgttgacgat
     7501 gtcggtcatg ttgaatgaac cggcctgcgc caccacgccc atcagggaaa gcccgaggaa
     7561 cacttcgtag ctcagggtct gcgcagaagc acgcatcgca cccagcaacg agtatttgtt
     7621 gttacttgac cagcccgcaa acagcaccgc gtaaaccgcc agacctgcca tcatcaggaa
     7681 gaacaaaatc ccgatgttca ggtcggcaac cacccaaccc ggactgactg gcacaatcgc
     7741 aaaggccagc agcagcgagg taaaggcaat catcggtgcc agggtaaaga tgacgcgatc
     7801 cgagaatttc gggatccagt cttctttaaa gaacattttg atcatgtccg caaccagctg
     7861 gagcgaaccg ccccagccaa cacggttagg tccgtaacgg ttctggaaca gacccagcag
     7921 gcgacgttcg ccaaagctca tgaatgcccc gcaggtgaca accaccagca ggatcaccac
     7981 cgctttgagg atggtcagca ggatctcaat cagttccggt gatatccaac tcattgttgt
     8041 gcctccttga gatcctcaag atgcgcgcca gccagcaccg gagcaatacc ggacataccc
     8101 atcggcaagc ccacctgccc tgccgtcagc ccttcagcga tttcaaccgg cagcgtgacc
     8161 gtgttgccat cgtaactaaa ggagacgtgt gtacctgcgt tcacacctaa cttcgcggca
     8221 tccgctgggt tgagtttgat gtacggctgc ggcatacggc tctggaagac cggagcacgc
     8281 tgtgacaatt catcgctgcc aaacaggtgg taatacggcg cgatacgcca tttcccgtcc
     8341 tgcggctgga agcgtgccgg tacgctggtg aagtaatcca gaccattttc gctggtttca
     8401 aacagacgca cgcccggatc gccaaagcgc agtttgccgc ccacttcgtc ctggaatttg
     8461 ttccacgcct gcggggagtt ccagcccggc gcccaggcaa acggcacttg cgaacggtgc
     8521 gcagtcggct ggttgttacc ttccatcgag aaggtgaaca tggtgtcaat atcctgcggc
     8581 tgacgcggct catgaacgct gatattggcg cgcatggcgg tacgaccgct gtaacggtgc
     8641 ggttcacggg ccagtttctg cccacgaata cggaatgtcg catccggcgc agcatctttg
     8701 atacctgcca gttccgggat tttcgccaca acagcgtcaa tcacatggtc gagctgcgtc
     8761 cagtccactt cacggctcag cagggtgctg tgcagcgagt gtaaccagcg ccagctttcc
     8821 agcatgacag ttttgctgtc gtaataagca ggatcgtaaa cctggaagaa acgttgggcg
     8881 cggccttcgt tgttgatcac cgtaccgtcg ctttcagcaa agctggcagc agaaagtacc
     8941 agatgggcgt tttccataat cgctgtgcgt tgatgatcaa ccaccatcac cagcggcgct
     9001 ttagccagcg cagcattcac gcgggtagcg gaagcgtgac gatgcagatc gttttccagc
     9061 accaccaccg cgtcggcgcg tccggtttcc agttcggtca acgcttcttc aagcgaaccg
     9121 ccacccataa tgcccagccc catgctgttg acggaacggg caatcatggt gataccaacg
     9181 tcagcgccgc gacctttcag ggctttcgcg acgttagccg cagcctgaat cacctctatg
     9241 ctaccggcgt tcgtcccgga gataatcaac ggtttcttcg cacctgccag tgcctgcacg
     9301 atgacgtcga ttttgctttg cagctcaggt tcgataccgt caaccgctgg cgcagagtta
     9361 tccagcgcat gggcgatggc aaaacctaaa cgcgcctgat cttcaaccgg tgcgcggtaa
     9421 gtccacgccg cgatatcatc cagacgggtg tcatcaacgt tggtaacaaa cagcggatgc
     9481 ttcgcacgtt gaccgatgtt gaggattgcc gcaatctgcc agtcagccac tttctgtgct
     9541 gccgccattt cgcgcgcttt acctttcaca gcctgacgca ctgccagcgc gacgcgcgcg
     9601 ccggtctggg taacgtcttc gcccagcacc agtaccgcat cgtaagattc gatttcgcgc
     9661 agagccggag tataaatgcc gccttcacgc agcactttca gcgccagttg cagacgttcc
     9721 tgctcaccgt gagcgatacc ggtgtagaag ttttcttcgc ccaccagttc acgcagcgca
     9781 aagttgcttt ccacgctggc acgcggagaa ccaataccga tcactttctt cgactgacgc
     9841 agaatatctg ccgcgccctg cattgcctgt tcggcgttga gggtaatgaa atcatcgcca
     9901 cgacgctgta ctggttgacg cggacgatct ttcaggttga cgtaaccgta accgaaacga
     9961 ccacggtcgc agaggaagta gtggtttacc gtaccgttgt aacggttttc gatacgacgc
    10021 agttcgccgt aacgttcacc ggggctgatg ttacagccga tggaacattg ctggcagatg
    10081 ctcggcgcaa actgcatatc ccatttacgg ttgtaacgct cggagtgcgt tttgtcggta
    10141 aatacgccgg tcgggcaaat ttcgaccagg ttaccggaaa attcgctttc cagcgtgccg
    10201 tcttccgggc gaccgaagta gacgttgtcg tgcgcaccgt aaacgcccag atctgtaccg
    10261 tcagcgtaat ctttgtagta acgcacacag cggtagcagg cgatgcagcg gttcatttcg
    10321 tgagagatga atggccccaa atcctgatta cggtgggtac gtttggtgaa acggtagcga
    10381 cggaagctgt gtccggtcat cacagtcata tcctgaagat ggcagttacc gccctcttca
    10441 cataccggac agtcgtgcgg gtggttggtc atcaaccact cgaccacgct ttcacggaac
    10501 tgtttcgctt cttcgtcgtc aatggaaata aaggtgccat cggaagccgg tgtcatacag
    10561 gacatcacca ggcgaccacg cgtgtcttcc gcgttttggt attgcttcac cgcacactgg
    10621 cggcaagcac cgacgcttcc cagcgccgga tgccagcaaa agtaaggaat atcaaggccc
    10681 agagacagac aagcttccag caggttgtcc gctccgttga cctcgtattc tttgccgtct
    10741 acatgaattg tagccattag catgcttcca gttttctcgg tcagggaccg agcgttaatc
    10801 gaaattcggt taccagcgct ctttcagcag gttcggctga atcccattaa tcaaatgggt
    10861 attgctgaac ggctgtttga ttcccgcctc aaattcttcg cggaaatatt tgatggcgct
    10921 ctgtaacggc tccactgcac caggtgcgtg ggcacagaaa gttttacccg ggcctaagaa
    10981 tcgacacagt tgctcaagtg tttcgatatc gcccggctga ccttcaccac gctccagcgc
    11041 acgcagaatt ttcacgctcc acggcagacc gtcgcggcac ggcgtacacc agccgcagga
    11101 ctcacgggcg aaaaactctt ccaggttacg caccagcgac accatgttga tctcatggtc
    11161 aaccgccatc gccagcgccg tacccagacg gcttcccgct ttaccgatac tttcgaattc
    11221 catcggcagg tcaaggtgcg cttcggtcag gaagtcggtg cccgcgccgc ctggctgcca
    11281 ggctttgaat ttcagaccgt cacgcatacc accggcgtaa tcttcgagga tctcgcgcgc
    11341 ggtagtacca aacggcagtt cccacagtcc cggatttttc acccgaccgg agaagcccat
    11401 cagcttggtg ccagcatctt tacttttcga gatgttctga taccactcca cgccgttagc
    11461 gaggatcgcc ggaacgttac acagggtttc gacgttgttg acacaggtcg gtttgcccca
    11521 tacgccggag gttgccggga aggggggttt cgagcgtggg ttagcacgac gcccttccag
    11581 ggagttgatt aacgctgttt cttccccgca gatgtagcgc cctgccccgg tatggacgaa
    11641 cagttcgaag tcgaaacctg ttcccataat gtttttgcca agcaagcccg cttcagtggc
    11701 ttcggcaatg gcgcggcgca gatttactgc cgcttcgata tattcgccac gcaggaagat
    11761 gtagccacgg taagctttca gcgcaaacgc ggagatgagc ataccttcca ccagcaggtg
    11821 cggcagttgc tccatcagca ggcggtcttt ataggtgcct ggctccattt catcggcgtt
    11881 acacagcagg taacggatgt tcatggattc gtctttcggc atcaggctcc atttcaggcc
    11941 ggtagaaaag cccgcgccac cgcgcccttt cagaccagcg tcttttacct gattaacgat
    12001 ttcgtccgga gacagcccgg tcagcgcctt acgcgcgcct tcgtaaccgt ttttgctgcg
    12061 atattcgtcc agccacactg gctgtttgtc atcgcgcaga cgccaggtca gcggatgcgt
    12121 ttcgggagta cggataatgt ttttcattta taccgctcca gcagttcagg gatcgcttcc
    12181 ggggtcagat gcgcgtgagt atcctcatcg atcatcatgt ttggcccttt atcacagttc
    12241 cccaggcagc aagttggcag cagcgtaaag cgaccgtcaa atgtcgtttg ccctggtttg
    12301 atgtttagct ttttctcgag cgccgcctga ataccctggt aaccgttgat atgacagacc
    12361 acgctgtcgc aataacggat cacatggcgg ccaaccggct ggcggaagat ctggctgtag
    12421 aacgttgcca caccttcgac gtcgcttgcc ggaataccca gcacatcggc gatcgcgtgg
    12481 atcgcaccat ccggcaccca gccacgctgc ttctgaacga ttttcagcgc ttcaatggac
    12541 gccgcacgcg ggtcttcgta gtggtgcatc tcgtgctcaa tcgcttcacg ctctgccgca
    12601 ctcagctcaa aagcctcggt ttgtggttgt tgattctcgt gcataattag cggtccacat
    12661 ctgacataac aaaatcgata ctgcccagat aaacaatcag gtcagacacc aggctgccgc
    12721 ggatcgccgc cggaatttgc tgcaaatgcg cataactcgg cgtgcggata cgagtgcgat
    12781 aactcatggt gctaccgtcg ctggtcaggt agtaactgtt gatccccttg gtcgcctcaa
    12841 tcatctggaa agattcattg gcaggcatca ccggccccca cgacacttgc aggaagtggg
    12901 tgatcagggt ttcgatatgt tgcagcgtgc gctctttcgg cggcggcgtg gtcagcgggt
    12961 gatccgcttt gaacgggcct tccggcatgt tgttgaggca ctgctcaaga atgcgcagac
    13021 tctggcgcag ctcttccact ttcagcatta cgcgggtgta gcagtcagaa acaccaccac
    13081 caaccgggat ttcaaagtcg aagttttcat agccagaata aggacgcgcc ttacgcacgt
    13141 cgaagtcgat cccggtagca cgcaggcccg cgccagtggt gccccactcc agcgcctctt
    13201 tcgcgccata ggcggcaacg ccctgggaac gacctttcag aatggtattt tgcagcgccg
    13261 ctttctcgta agacgccaga cgtttcggca tccagtcgag gaactcacgc agcaggcgat
    13321 cccagccgcg cggcaggtcg tgcgctacgc cgccaatacg gaaccacgcc gggtgcatac
    13381 ggaaacccgt gatcgcttcc accagatcgt aaattttctg acgatcggta aaggcgaaga
    13441 acacgggcgt cattgcgccg acgtcctgaa taaaggtcga gatgtacagc aggtggctgt
    13501 tgatacggaa cagttcggag agcataacgc gaatgacgtt aacgcgatcc ggcacggtga
    13561 tcccggccag tttctctacc gccagcacgt aaggcatttc gttaacgcag ccgccgaggt
    13621 attcaatacg gtcggtatac ggaatgtagc tgtgccagga ctggcgctcg cccattttct
    13681 ccgcaccacg gtggtggtag ccgatgtctg gtacgcagtc gacaatctct tcgccgtcga
    13741 gttgtaaaac gatacggaaa gccccgtgcg ccgacgggtg gttcggaccg aggttgagga
    13801 acatgaagtc ctcgttttcg gtgccgcgct tcatccccca ctcttccggt ttgaaggtca
    13861 gggcttccat ctccagatcc tgtttggctt tggtcagctc aaacggcgag aattcggtag
    13921 cgcgcgccgg gtaatcttta cgcagcgggt gacctttcca ggtttgcggc atcatgatgc
    13981 ggcgcaggtt cgggtgaccg tcgaaagtaa tgccaaacag atcccaggtt tcacgctcat
    14041 accagttagc gttcgggaac agtttggtga aggtcggtac gtgcaggtcg ttttctgcca
    14101 gcgccacctt cagcatgatg tcgcggttac gatcgataga aatcagatgg tagaaaacgg
    14161 aaaaatccgc ggcaggtaac ccttcgcggt gtgtgcgcag acgttcgtcc atgccgtgta
    14221 agtcaaacag catgacgtaa ggtttcggca gtttctttaa gaaatcgcca acttccagta
    14281 attgttcacg cttgatccac acaacgggaa ccccggtgcg agtcgcctga acagtaaagg
    14341 catccggccc aaaacggttg cgcagttcgc caatcaccgg atcatcaaga tgatcgcggg
    14401 tctgccaggc gggttcttgc gcggttaagt cggtcatatt gttcaccatt gcaaatggtc
    14461 cgtggtgact gtcggcgcag taagcttcgc gaaatagatt taggaataag cgaagaaaaa
    14521 tccctttgcc gacaggcgca aattaaatct cgtcaggtgt acgcaggttg gttacggcaa
    14581 tgcgttcacc gcgcttgcgt tcgcgctctg attgcatatt ggcgcgataa acgccctgat
    14641 cgccaaccac ccaggagagc ggacgacgct ctttgccgat agattcctgc aacagcatca
    14701 gtgcctgcat gtacgcttca ggacgcggcg ggcagcccgg gatatacaca tcaaccggga
    14761 tgaatttatc gacgccctgc acaacggaat aaatatcgta cataccacca gagttggcac
    14821 aggcacccat tgagataacc cattttggtt ccagcatctg gtcatacaga cgctgaataa
    14881 ccggtgccat tttggtaaag caggttcctg caaccaccat caggtcagcc tgacgcggcg
    14941 aagcacgcaa tacttctgcg ccaaaacgcg ccacgtcatg caccgcggta aacgaagtca
    15001 ccatctcaac gtaacagcag gaaagaccga agttatacgg ccaaattgag tttttacgac
    15061 cccagttaac catgtcattg agcttgccca taaacacgtt tttgttaact tcttgctcca
    15121 gagggtcggt tacgatctcc tgcttttgca gggggtaacg gtcgttctca ccgttgggat
    15181 ctatgcgggt gagcgtataa tccatcttaa tgcctcgcgg ttagcgttga cgattagcga
    15241 tactgttcgt ttccgggttc atacgctcgc ggcgtgaacg cgcgggcgtc cagtccagcg
    15301 cgccaatacg caccagataa accagacctg ccagtaacac aaaaataaaa attgcagctt
    15361 ccacaaagcc tacccagccg ctttcgcgga tagaggttga ccatgcgaac agatacagcg
    15421 cttcaacgtc gaagataacg aagaacatgg ccaccagata aaacttggcg gacaggcgta
    15481 agcgggcgga gccgaccgag tcgataccgg attcaaacgg cacgtttttc gacctcgcgc
    15541 gtgcgcgacc gcctaaaaac caaccgccta ccagcatcag gcaacacagg ccaatggcaa
    15601 cgataagaaa gatagcgaat gcccagtgat gagcgatgac ttcagtggat gttgacatac
    15661 tcattgctta ctcatcaaaa gtagcgccag attcactgct cttcacggca gatggacgcc
    15721 acatcgattc atggggagga ataaaaaaaa ccttacaatc actgtagaaa ttcttttata
    15781 cagctaattg atgtggtctt ttactccttt ctataacctt ttgtcaactt taacaaaagt
    15841 ttcttcacat tagtttacat aatatcaaca ccattagcat ttaatgccct ttcaccccag
    15901 atccttgacg actccaggat aattagatgt tgttgaatcg tgtccgttgt gaagcaatgg
    15961 aaaaaatacg ggtctatttt gacaggaatt tgtgtcgatt cctcccccca aaagagagta
    16021 ttttcttgat ctgtgacacg cttttgtcat tccataacaa aaacgcagca acaaatttac
    16081 gtatttttta acatcattgt agcaggtgat ttttttcagg cgattatttg tgcgttcggg
    16141 acgtgaatct ctggtgattg aaaaatgaac agttttgtac gttctgcact atgcgaggaa
    16201 ggatttttac taaaaaaaag ccgctggggt ttaaaacacc cccagcggct cgttttttac
    16261 actattgtct caggaattat ctatcgtccg tcgattactc gatatccctt tcaatcaaca
    16321 atgaatcatc cccttccgga gcagacattg ggctgtattg ccacggatta tggtagctct
    16381 ccatcgcctg atagatcacc tgggccagct cattattact ggacgggtca tagcacagca
    16441 aatattcggt atcaggtagc ggcggtaagc catctactcc actcagcaca cgcaggtccg
    16501 ggctcatcat ctcaaccggc cttgccgtca cgccaagacc ggctttcact gccgcacgaa
    16561 cggccggaag cgtcgaggcg acataagcca gtcgccatgg aatatctgct ttattaagcg
    16621 tcgccagcac catatcgcga aacggacttg gatcatccag caatacaaga gggagcggct
    16681 cgcctttttg caatacgtat tccgctgcgc agtaccagtg tgttggcgag gttcgcaggt
    16741 tcaatgcctt aaacgctgaa ggacgatggg tggttaccat caaatccact tcctgggatt
    16801 ccagcatttc tgccatatag gcattacgtt taacgcggac atccagcgct aatttcggat
    16861 aaaccgaact cacgcgattt aacaggaaag gtaagatcgt atcggcagat tcatctgaag
    16921 caccgatagt taacacgccc tgaagattac tgaacattaa tgatgagcag gcctcatcat
    16981 taaaacgcag gattttcctg gcgtaaccaa gaagttgaat gccatgttca gttaacagtt
    17041 tgttgcgacc gtgacgagcg aacagttctt tcccaacgag ttgttccaga cgctgcattt
    17101 gctgacttac ggcggactga gtacgacaca cagcggcagc tgcggcagca aaagtgttca
    17161 gatcggcaac agcaacaaat gttctcagca gatcgaggtc gaggttaatt atcggacgat
    17221 ttgcacttat catatattat cacttactgg cggctcatac tgagctggtt aatgctgtgc
    17281 acacacaaac aagcaattcc atttgtaatg tgcctccctg gcagtttcat ccggaacccg
    17341 gacgaaagta aaaatgcata tgagttgcac taaaaaagcg actcacattg ttccgttata
    17401 atgcctgaag tagatcacag aatatatctt cagggatcgc atatctatta agttactcac
    17461 tcttttctat ttatgacatg cgcgtgtttg tataaatgta aatgtgagtc cttgttccac
    17521 tctcgtgcag catcgctggt catacgcgaa cacgtacaaa cagcaatggt gaggcggcat
    17581 caagagcagg gatccgtcat ttatgcgagc attttacccc aaaaaccttt tatttataag
    17641 ggtcattgcg aattatctga tgcaaagtt
//
LOCUS       DAZGVQ010000109        17453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000109 DAZGVQ010000000
VERSION     DAZGVQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.110"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..747
                     /locus_tag="ACHLAG_003482"
     CDS             <1..747
                     /locus_tag="ACHLAG_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP0656646.1"
                     /translation="STLLQIDTLIRQLAEISAMTESIGGKTALDWAMKQDFRCGCWLM
                     EKPETAMKAITRNLDREIWRDLMQRSGMLSLMDAHARDTWYRSLEYDNFPEISKANIL
                     STFEQLHRSKDEVFERGVINVFRGLNWNYKTNSPCKFGSKIIVNNLVRWDRWGFHLIS
                     GMQADRLADLERMLHLLSGKPIPNNRGNITINLDDHIRSVQGKERYEDEMFIIKYFKK
                     GSAHITFKRLELIDRINDIIARHFPSVLSA"
     gene            complement(1028..1564)
                     /gene="yghD"
                     /locus_tag="ACHLAG_003483"
     CDS             complement(1028..1564)
                     /gene="yghD"
                     /locus_tag="ACHLAG_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFP0656647.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLRSAAPDIMAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(1566..2744)
                     /gene="gspL"
                     /locus_tag="ACHLAG_003484"
     CDS             complement(1566..2744)
                     /gene="gspL"
                     /locus_tag="ACHLAG_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFP0656648.1"
                     /translation="MSSILEIFFPLCASDTVRWQRRTPDVEHGIWPDVADEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(2741..3718)
                     /gene="gspK"
                     /locus_tag="ACHLAG_003485"
     CDS             complement(2741..3718)
                     /gene="gspK"
                     /locus_tag="ACHLAG_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFP0656649.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLTQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDDGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVLLEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(3715..4320)
                     /gene="gspJ"
                     /locus_tag="ACHLAG_003486"
     CDS             complement(3715..4320)
                     /gene="gspJ"
                     /locus_tag="ACHLAG_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFP0656650.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNNAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGEQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQLS"
     gene            complement(4317..4688)
                     /gene="gspI"
                     /locus_tag="ACHLAG_003487"
     CDS             complement(4317..4688)
                     /gene="gspI"
                     /locus_tag="ACHLAG_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFP0656651.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(4685..5248)
                     /gene="gspH"
                     /locus_tag="ACHLAG_003488"
     CDS             complement(4685..5248)
                     /gene="gspH"
                     /locus_tag="ACHLAG_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFP0656652.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRQGQWSPVSSTRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(5252..5707)
                     /gene="gspG"
                     /locus_tag="ACHLAG_003489"
     CDS             complement(5252..5707)
                     /gene="gspG"
                     /locus_tag="ACHLAG_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFP0656653.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(5724..6947)
                     /gene="gspF"
                     /locus_tag="ACHLAG_003490"
     CDS             complement(5724..6947)
                     /gene="gspF"
                     /locus_tag="ACHLAG_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFP0656654.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAISEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDALQASGVYWLAGLLALLVLGQRLLKNPTMRLRWDKTVLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(6947..8440)
                     /gene="gspE"
                     /locus_tag="ACHLAG_003491"
     CDS             complement(6947..8440)
                     /gene="gspE"
                     /locus_tag="ACHLAG_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFP0656655.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(8440..10500)
                     /gene="gspD"
                     /locus_tag="ACHLAG_003492"
     CDS             complement(8440..10500)
                     /gene="gspD"
                     /locus_tag="ACHLAG_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFP0656656.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEELVDVLKQVSGTLTAAKEEAEGTVGSGREIVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(10530..11489)
                     /gene="gspC"
                     /locus_tag="ACHLAG_003493"
     CDS             complement(10530..11489)
                     /gene="gspC"
                     /locus_tag="ACHLAG_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFP0656657.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSLNTDKEHLRKIVRGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAATNKKAVSDEAKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(11507..11917)
                     /gene="gspS2"
                     /locus_tag="ACHLAG_003494"
     CDS             complement(11507..11917)
                     /gene="gspS2"
                     /locus_tag="ACHLAG_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFP0656658.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLISYQRQMCADPTVKLM
                     LTEGINYSITINNTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(11983..12792)
                     /gene="pppA"
                     /locus_tag="ACHLAG_003495"
     CDS             complement(11983..12792)
                     /gene="pppA"
                     /locus_tag="ACHLAG_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFP0656659.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMPSAQSKISLALPRSHCPHCQQTIRIRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASVIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGSW
                     VGPLSLPNVALIASCCGLIYAVIIKKGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(12932..>17453)
                     /gene="sslE"
                     /locus_tag="ACHLAG_003496"
     CDS             complement(12932..>17453)
                     /gene="sslE"
                     /locus_tag="ACHLAG_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFP0656660.1"
                     /translation="SATLLAGCDGGGSGSSSDTPPVDSGTGSLPEVKPDPTPNPEPTP
                     EPTPDPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSLRVTGDITCNDESSDGFTF
                     TPGDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDAQELAGSDNKKSNALSL
                     VTSMNSCPANTEQVCLEFSSVIESKRFDSLYKQIDLAPEEFKKLVNEEVENNAATDKA
                     PSTHTSPVVPATTPGTKPDLNASFVSANAEQFYQYQPTEIILSEGRLVDSQGDGVVGV
                     NYYTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKSTIALTELGDEVRGANI
                     DQLIHRYSKAGQNHTRVVPDEVRKVFAEYPNVINEIINLSLSNGATLGEGEQVVNLPN
                     EFIEQFKTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQIQGVINKLWGVDTNYK
                     SVSKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYWLAFGEKRAWDKN
                     ELAYITEAPSLVEPENVTRDTATFNLPFISLGQVGEGKLMVIGNPHYNSILRCPNGYS
                     WNGGVNKDGQCTLNSDPDDMKNFMENVLRYLSDDKWTPDAKASMTVGTNLDTVYFKRH
                     GQVTGNSAAFDFHPDFAGISVEHLSSYGDLDPQEMPLLILNGFEYVTQVGNDPYAIPL
                     RADTSKPKLTQQDVTDLIAYLNKGGSVLIMENVMSNLKEESASGFVRLLDAAGLSMAL
                     NKSVVNNDPQGYPNRVRQQRATGIWVYERYPAVDGALPYTIDSKTGEVKWKYQVENKP
                     DDKPKLEVASWLEDVDGKQETRYAFIDEADHKTEDSLKAAKEKIFAAFPGLKECTNPA
                     YHYEVNCLEYRPGTGVPVTGGMYVPQYTQLSLNADTAKAMVQAADLGTNIQRLYQHEL
                     YFRTNGRKGERLSSVDLERLYQNMSVWLWNDTSYRYEEGKNDELGFKTFTEFLNCYAN
                     DAYAGGTKCSADLKKSLVDNNMIYGDGSSKAGMMNPSYPLNYMEKPLTRLMLGRSWWD
                     LNIKVDVEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQSTGLWAPAQKEVTIKSNA
                     NVPVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTVKFKVPYGGLIYIKGNS
                     STNESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESDAFVYTTPKKNLNASNYTG
                     GLEQFANDLDTFARSMNDFYGRDETSGKHRMFTYKALTGHKHRFTNDVQISIGDAHSG
                     YPVMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATEVANNVLALYMQDR
                     YLGKMNRVADDITVAPEYLEESNNQAWARGGAGDRLLMYAQLKEWAEKNFDIKKWYPD
                     GTPLPEFYSEREGMKGWNLFQLMHRKARGDDVGDKTFGGKNYCAESNGNAADTLMLCA
                     SWVAQTDLSEFFKKWNPGANAYQLPGASEMSFEGGVSQSAYNTLASLNLPKPKQGPET
                     INKVTEYSMPAE"
BASE COUNT         3907 a         4951 c         4405 g         4190 t
ORIGIN      
        1 agtacactgc tgcaaatcga cacacttatc cggcaactgg ctgaaatctc agcaatgaca
       61 gaaagtattg gcggtaaaac cgcactggac tgggcgatga aacaggattt tcgctgtggc
      121 tgctggctca tggaaaaacc tgaaacggca atgaaagcca tcacacgcaa tctcgatcgc
      181 gaaatctggc gtgacctgat gcaacgttcc gggatgctga gtctgatgga tgcgcatgcc
      241 cgggacacat ggtaccggtc actggaatac gataactttc cggaaatcag caaggcgaat
      301 atcctgagta cattcgaaca actgcaccgg agtaaggatg aggtgtttga gcgcggggtg
      361 atcaacgtct tcagagggct gaactggaat tacaaaacca attccccctg taaatttggc
      421 agtaaaatta tcgtcaacaa cctggtgaga tgggacagat ggggatttca tcttatcagt
      481 ggaatgcagg cagatcgcct ggctgacctg gaaagaatgt tgcatctgct cagcggtaaa
      541 ccgatcccca acaaccgggg gaatatcacc attaatctgg atgaccacat acggtccgtt
      601 cagggtaaag aacgctatga agatgagatg ttcatcatta aatactttaa gaaaggatct
      661 gcacacatca ctttcaaaag gctggagctg attgacagaa ttaacgatat aatagccagg
      721 cactttcctt ctgtgctctc agcctgaccc cgagtttgat tccctttcga tatcaaaagg
      781 gactgcgggt acaaaagagg gtacatcttt caccaaacca aacaaaataa aataatatca
      841 acatgataga agcaatcttc gattccgagt ccggcaccac taattcttaa gaacccgccc
      901 acaaggcggg tttttgcttt tggatctgac aataaccttc acgaaaaaag tttgcttata
      961 aagtctgggg gaattactct cgccacgtta acgagagtaa ttttattgat actaatctcc
     1021 tgatacttta cccccgtcca aactccagcc gctgcacatt caccatccca gtcttctcag
     1081 cagcactggc atcaatttgt gtcacccgca gcgcatactt ttcatccagc gcgtttaacc
     1141 agttcagcag gtcattaaac accaccggat ctatccagac ctgaatattc tccccacgct
     1201 cggctatccg cttgatgact accgagtgcg cggaagcgct gtcactgata acccgcgata
     1261 cctgcgcagg cgttgttgtg gcggattttc gcgctgccat aatatccggc gcggcgctcc
     1321 tcagtctggt gttcatcgcc accagttgct gcaacattgt ctcctgttgt tctatccgtt
     1381 cgctcaacgg ctgccagatg aggacgtaat atccgacgct aaacaggaac accaccgctg
     1441 ccagtaacat gcccttttca cgcggagaac gccccgccag gtgttgtgcc agccagtgtt
     1501 cgccacggct taactggcgt tcacgccatt gctgaaaata gtgaataaat ttatcgcgta
     1561 acatgttatt tcctccgcaa cgttacgccg ccggaaaccg catcaccctc tttctgtaat
     1621 gcgtcctgtt gcacaacata atcggttgcc agtgcactac gcagtttgtc gaaactggca
     1681 aagttagcag cccgcaactg gaggtgaagc gtctggcgtt tttgatcaaa ggtgaaacca
     1741 cgcatttcga tgtcggaaag tgacgctgat ttcagggtgc tggctatcgc tgacaactcg
     1801 gcgagcagcc gggtatcgtc ggcctgtggg cgatattttt tcagcgccat cgtcacctga
     1861 gagcgtaaat tcacaatccg cttctgctcc gggaacaacg ttaagaactg ttcctccgcc
     1921 tgggtgcggc tttgcgccac ctgttcgctg acgctccata acgtcacgcc ccgctccact
     1981 gccagcgcaa ccagaatcag caatatcggc agaatcatca cccgccagcg cgcccactgt
     2041 tttcggtagc tgacgcgagg ctgccacggc cctgtcagca ggttcccttc cggttcgcca
     2101 taagtggtaa cggcgggcag agctgtaacg gtcaggcgtt cagcgtctgc gccagcccat
     2161 gctgatagct tttccggtgc aatgccgact acggttagcg aaagcggtaa atcctgctca
     2221 ttgagctggg cgcggaacat gaccggagcc agcgccagcc cggcgctcca tccccggcat
     2281 tcatcgatgc ggcagataac ccgttgcgca tcgccagcca taaacccaca aggaatggac
     2341 atccagtccg gcgcgacggt agcgcgggtg atgccgtttt cctgcaacca ctgcgcaatg
     2401 ttgcgcatat gctgttggtg aatcactgct acggttgcca gttgctggtc gattttcaac
     2461 ggggcgaaat gcagttcatc gatatcctgg ttcagctctt cttccagcaa ggcgggcaga
     2521 atagtcggta tctgcttgcg ggcgacatca ggcagttcaa cctgccagac gctgatccat
     2581 tcgccgggaa tgtagagtcg aatcgcatcg gtttgcagcc attgctgaag ctgttcgtca
     2641 gcaacgtcag gccagatacc gtgttccacg tcgggtgtac ggcgctgcca gcggacggta
     2701 tcggaggcgc aaagcgggaa aaaaatctca aggatggaac tcactcactt tctcctgtct
     2761 gatgccagag aaccgaaaag tgttgtgggc ccatgcggac aattaacgaa ttcatcgtca
     2821 gttcaatctc attcacggtg atatctgaac gcagccagaa gtaattgctg tccacgctca
     2881 ggacggtttt tagctgtttt ttagtacgct catcgacgtc agcaagtagc ggctgtgcaa
     2941 gaaactgatc gacatcttcc cagcccttcg ccggacgttg ttgtaataat gcccgcgcct
     3001 gaacagggct taaccacggg tcaaacagcg cctcaagaag cacactttgc gtgacgtcta
     3061 aggtattgat gttgatttgc tggcgggtca tcggcagcgc acagaccagc ggtttcagtt
     3121 tttgataaag cccgtcgtcc attccctgca ccacgcgcat ctcgctgata tcggccagcg
     3181 gttgattcgc ggcgtagaaa ggcaccgaac gggcgagata ctcgctgtct tcacggccca
     3241 gacgtgtctg gacgctgcgg tcctcgtcaa taaactccca caggctttcg gctatcagtt
     3301 cggcccgata agcaggcaca tccaggcgcg agatcagggc aatcaactgt tgtaccgcaa
     3361 gcggacgcga cgccgttgtc ggctgagcaa gggcattcag gttaaagcaa gcctgtgcgt
     3421 cacgcagggt gaccgcaatt tgcccctgcg gcagagggaa gaaacgaggg ccggatgccc
     3481 acggctgtgt cagatgcacg cgcttttcat ttttcaggct cagactgagc gcgctcagcg
     3541 ccaggctttc cgcactggcg ctgtaccaca gcgcctgctg gtactcctgc tggctgcgcg
     3601 ttcgcccaag ttgttgctgc atccgcccgg aaagcgtgat ggttaccagc atcataaccg
     3661 ccagcaatac cagcaccacg accagtgcca ttccgcgttt tggtggtgag gtgatcatga
     3721 taattgcggc ccgcgtaaca accagatgcg ttcaatctcg ccccattgcg gcgaatgcag
     3781 agtcatgcgc actgccacag ggatcgcctg tactgacgac cagctctcct gccagcgcgt
     3841 accatcatag aactgcaaac ggagcgaatc cgccggaatc aacttttgca ttgtcggctt
     3901 cacgctgcct gcggcatcgg tcagcggcca ggctaaccgt tcgagataac cgtcatgaat
     3961 gcggtaacca acggtgagca gattgctgcg tggcagacgc atcaacgggt taaccacgcc
     4021 gccgcgcaca aagcgcatgc cttcactctc agacgccagc acgccagcgc ccgccagtaa
     4081 cgctggttca cgctggccct gttcgcctct taccggacgc ggcatcattt gtgtcagatc
     4141 gtgggtcaga aaactcatcg tttgctgcat gaggtttagt ttttgatcat gtccggcgac
     4201 ggcgttattc acgcgtgtga caccgtttgt cacctgctgc gccatcagtg ccagcgaggc
     4261 aaaaatggca attgccacca acatttccag taacgtgaaa ccagcgcgag tccttctcac
     4321 tgttggcctc ccacggcgct aaaccaggcg cgtcgtgact gaatcaccga cgaaaagtct
     4381 tcgtgacggc tgacttcaat atccaccgcc tggagcagcg cgttgcctgt attcaatggg
     4441 attgttcgcc agtaccagcg gcttcctgcc atatcgctct cgccctgatg cctgaccgcc
     4501 cgctgctcac gggtcatcag gtagagcagc gcagtctggt tttcagctac ccagcccgct
     4561 accgtttttt cctcaagaac gtgctgatta ctcagcgcac cgctggcaat ctgtaacacc
     4621 gccgtggcag ccagcgcaaa aatcgctagc gcgagcatca cttccagcaa ggtaaatccg
     4681 cgcttcatgg catcctctca tcacattgat tgagggataa cgcgccatcg tgtgccagct
     4741 ttaccgccca acatgcgttt tgcgccgccg agtagaagcg cagcgtaaac ggcgtggcgg
     4801 gttcaaaagg cgaaaaacgg atctgcggag tcttcttttt cgcctctttt tgcagctcca
     4861 gttcaatatc gtgcagcgtc agtcgacgac gttgcagctc cagcgcatat tccttctgcc
     4921 agatatcact tccgggttgt aacagcatct gcacctgttt cggcaccgta acctgtgccg
     4981 ataaccgggt cgaggaaacg ggcgaccact gcccctgacg acgctgcata aactggtagc
     5041 caggcgggtc gattagcaca ccgagtgttt tcccttcgat cactgccctg tccttaaact
     5101 gcgcaaagcg agtcagaaaa tcctgcgccg cttttttcgc gggcgattca gaatcggtcg
     5161 caaaagtctg taccacgccc gcactggcaa ggccgataag aaaaatcacc agcatgattt
     5221 ccagaagtgt gaatccgcgt tcaggcactg cttactgaaa ctcctgcaaa ttccagttac
     5281 cgatatctgc gccagcgcct tccccgtttt cctgaccatc cgcccccagg gtataaacat
     5341 caaacagccc tttttcaccc ggactgagat actgataatc attgccccac ggatcctttg
     5401 gcagtcgttt aatgtatcca ccagtacggt agttgcggga gtccgccata ttggctggct
     5461 gctggatcag cgcctcaagc ccctgttctg tggtcggata acgcccgtta tccagtcggt
     5521 acatatccag tgcgttttcc agcgccacga tatcgctgat ggctttttgc cgatcggctt
     5581 tctctttgtt gcccaacagg ttaggcacca ccagacttgc caggacgcca agaataacaa
     5641 tcaccaccat cacttccagc agggtaaaac ctgcccgtgg tttttgtgtg cgggataacg
     5701 aattcatgtg ataactccgt aaattacatt ccaaccatat tattcagttg cagcatcggc
     5761 tcgaggatgg cgatgacaat aaacagcacc acgcccgcca tcatcaccac cagcgcaggc
     5821 tcaaacagcc ccaacgccag ccccacctgg gtatcaaatt cccgttcctg gttaacagcg
     5881 gcctgctcaa gcatggtttc cagttcgccg ctctgttcgc cggaggcgat catgtacagc
     5941 atcatcgggg ggaacaggcg caactccgcc agcgcggcgc gcagactgct tccttcgcgg
     6001 acgcgatctg ccgccagcag tagttgttgt tcgacatagc gatttgccga cacagcggca
     6061 gcggtctgaa taccttccag tagcggaaca ctgctggcgg tgaggatgct taaggtgcgg
     6121 gaaaaacgcg ccgtattcag cccgcgcgca acacgccctg tcacgggcag acgcagcacg
     6181 gttttatccc agcgcaggcg catagtagga tttttaagta gccgttgccc cagcaccaga
     6241 agcgccagca atcccgccag ccagtaaacg ccgctggcct gtaacgcgtc gctcatggca
     6301 ataagcgtgc gggttgaggc gggtagcgcg tgtccgagat gatcaaactg ttcgatgatt
     6361 ttcggcacca ctgccgtcag caaaatagtg actacgcccg ttgccaccac cagcagaacc
     6421 agcggataga gcatggcctg caatagacgt gatttcaggc gctgtcgctg ttcggtgtaa
     6481 tctgccaggc gattaagcac cacgtcgaga tgcccggatt tttctccggc agcaaccatc
     6541 gaacaaaaca gagaatcaaa gacgcgggga tgttcgcgca ggctgtccga cagggtgtaa
     6601 ccttcctgaa tccggctgcg cagcgccatt ccgaggcttt ttacatgcag tttttcactt
     6661 tgctcactga tcgcctgtaa gcaggtttcc agcggcattg ctgcctgtac cagcgttgcc
     6721 agttggcgcg tgaacagcgc aagatctgcc gccgccacgc gacgatgtgc gtgccgccga
     6781 cgctgcaaca tcccccctga cgaagtattc atccgggctt caatatgcac ggggataagc
     6841 tctttaccgc gcaacaactg gcgggcatga cgcgcggaat ccgcctcaat catacctttg
     6901 gttttgcgac cattacgctc cagcgcctga tagtaaaaca gtgccattac gcctccatgg
     6961 ttacccgcag aacttcatcg agagaggttt ctccggcgag cactttctca atgccgttgc
     7021 tgcggatacc cgcagagtgt tgtcggacat aacgttccag ctccagctcc ccggcctgac
     7081 ggtggatcaa atcacgcaat gtggcatcca ccacgatcag ctcatggatg gcagtccgtc
     7141 cgcgaaaacc tttgtgatta caggcgggac agccctgtgg atggtacaga gtgacggtac
     7201 gggcgtcggt aattcccagc aggcgttttt cttcgtcagt ggctggtgcg ggctggcggc
     7261 agtcgggaca cagcgtgcga accagtcgct gcgccatcac gcccgtcaga ctggaagaga
     7321 gcaggaaagg ctccacgccc atatcctgca aacgtgtgat cgcccccacc gctgtgttgg
     7381 tatgcagcgt ggaaaggacc aggtgtccgg taagtgaagc ctggacagcg atttctgcgg
     7441 tttcggtatc gcgaatttca ccgaccatca ccacatccgg gtcctgacgc aaaatcgcgc
     7501 gcagcccacg ggcaaaggtc atgccgacgc gggtgttaac ctgcgtctga ccgatccctt
     7561 caatcatgta ttcgatggga tcttcaaccg tgagaatgtt gcgcgaatgg ttgttcagct
     7621 cctgcaatcc ggcgtacagc gtggtgcttt tgccggaacc cgtcggcccc gtcaccagaa
     7681 agatgccgtg cggtttgtgt aacagctggc gcaactgcgc ggtcagttgc tggctaagcc
     7741 ccagacgctc cagcgtcagg cgggcctggt ttttgtccag cagtcgcagc accacgcgct
     7801 cgccccaggc agaaggcatg gtggagacac gcacgtcaat cgcccggccg cccagcaaca
     7861 gcgcaatacg tccatcctgc ggcacgcgct tttcggcaat gtccagccgc gccatcacct
     7921 tgatacgcga caccagcagc gaggccagtt tgcgccccgg acgcagcatt tcatgtaatg
     7981 tgccgtcaac acgaaaacgg atcaccagac tcttttcaaa cgtctcaatg tggatatccg
     8041 aagcgccttc tttgattgcc tctgccagca tggcgttgat cagtttgatg atcggcgcat
     8101 cgtcgtcgct ttccagcaga tcttccgttt ccggcagttc ttccgcgagg gtaaaaaagt
     8161 cctcggcaga accgagatct tccatcagct ggcgagcctc ggaagagtcc cgctgccaga
     8221 ccgcattcag ccgctgttca aattcggcct cgtcgatttg ccgcagcgta aagggcgcgt
     8281 tcagcccccg ctgcagctcc tgcaaaacag agagcgacaa cggatgaaca tggaggatct
     8341 ccagcgacgc ttcgcaccat gccaccaggc taaaccgacg gctgaaactg tagggcagac
     8401 gcacggtgtt agcggtggtt tcctgtgcta caggcaccat taacgcgttc tcccggcatt
     8461 aaggaacgca cgaacttccg gcggtagagc ctgattttgc gctggcagta ccggctgcgc
     8521 ggtgtgcggc atcaggctta aaccttgctc atcacgatag atctgttcgg cgcgcatata
     8581 gttatatttg cgctgcgaca cgccgtctgc cgccataccg tcacgcagaa tggtcggacg
     8641 gataaacacc atcaggttac gtttttcttt tttatccgcc gtcgatttaa acaggttacc
     8701 aatcaatggg atatcgccaa gcagcggcac tttcgccacg ctttctcccg cctggtcgtc
     8761 catcagaccg ccaagcacaa tcagctcacc atcgttagcc agcacggtgg ttttcagttt
     8821 gcgctcacca aacaccacgt cgaggctggt ctgtccttcc accttcgaga cttcctgctc
     8881 aatcaccatc tgtaccgcgt taccttcgtt aatctgcggc gtgactttca gcatgatgcc
     8941 gacttttttc ctttctactg tattgaaagg attgctgtta ttggagccaa cggtagagcc
     9001 ggttaatacc ggaacgtcct ggcccaccat gaagaaggct tcctggttgt ccagcgtggt
     9061 gatgctcggc gtggagagca cgttagagct ggagtcgttt ttaaccgcct gcaccagcgc
     9121 catccagtcg cctttcacca caccaaccgc cgtaccgcta aagccggaaa gaagctgggc
     9181 gagtgtggag agatcgccgt tggtatccgg attaatggtg gtagcgccgt tttcactgat
     9241 caccgtcgag cctttctgcg gtttcgcctg agaaatggct gcccccagcg taccgatagg
     9301 gatctgcgta ccattagcaa actgcattaa tccggcatct ttcgacgccc actgcacgcc
     9361 gaagttgata ttgctacctt cggcaacttc cacgatcaac gcctcgacgt gcacctgagc
     9421 acggcgaata tccagttgtt caatcacgct ttgcagcgac tgcatgatgt cctgcggtgc
     9481 ggttacaatc agggcattac tgtgtttgct ggcggcgatg gatacaatct cacgcccgct
     9541 accgaccgtg ccttccgcct cttctttagc cgccgtgagc gtgccgctga cctgcttcag
     9601 cacatcgacc agctcttcgg ctttgctgta tttgagatag aacacctggc tgttgccgct
     9661 gcgttccatt tctgagtcca gccgacggat caggcggcgc attttgtccc gcgtggccgg
     9721 gtcaccactg acaatcacac tgttggtgcg ttcgtcggcg acaatttgag atttcagcgt
     9781 cgcaggctgg ttctcgccgc tgtttttcgt caggctttcc agcacgcggg cgatttccga
     9841 agccgaggcg ttatccagcg ggatcacctc ttcggtgcga ttacccgcgt gatccacgcg
     9901 ctggatcact tctgtcaggc gctccacaac ggaggcgcgt ccggtgagca taatcacgtt
     9961 ggagggatcg taattaacaa cattgcctga gcctgcgctg tcgatcatct ggcgcagaat
    10021 cggtgccaac tcgcgtaccg acacattacg taccggcact actttggtga ccatttcatc
    10081 gcccgcgtag ttgtcgctgc cttcaccgac cagcggcagc ggctcgactt tcgcagcgct
    10141 ggatttcacc accttcagca cgtcgttttc catcggtacg acggcatacc cctgtgcttc
    10201 cagcaggtta aggaatagct ggtaatactg gcgttcattg agcggggtca tggtgcgaat
    10261 actcactttc ccctgtacgc ccggccccat aatgatggtt ttattaaggt tagcgccgac
    10321 ggtttcgatg aacgatttca ggtcggtatc tttaaaatta gcggtgaaag tggcttcttc
    10381 cgcccagacc ggtgaactgc ataatgccgc tgccagcacc agcggcagta aacgcttttt
    10441 tgttttgagg ccagttgtct tcttacgcca gaccgacaac gtaatatcac gccaaaacac
    10501 gatgaatgat tctcctggat taaatgcggt tagcgcagcg cgatggaaat gtcgtggcgc
    10561 gcacccttgc gtaaaaccgt aagttgaatg gaatccattg aaggtaactg ccgcatcaga
    10621 gcaatcattg ctcgtggatc agtgaaatcc tgctgattta gcgcaatggc gatatcgcct
    10681 tccttgaaac cgctggcatc gaacagagaa cgatctgccc ccggcttcac tgcataaccg
    10741 acaattccct ccttacgcac aggcgtaagc tggatatagt taaaaatttt ctgcggatct
    10801 ttcgccagtg cctgacgcac ggcagtcggt atctcaactg gcgcactgac agcaggttca
    10861 gcaacagctt gctttgcttc gtcactgaca gcttttttgt tggtcgcggc aacggtggaa
    10921 cgctcctctt cagccaggct caggcgctct atttttccct gatagcgcag catcacatgg
    10981 tcgcgattga tttcctcaat caccgcattg tgcgagccaa gcgtttcacc ctgcaaatag
    11041 acctgctgtt taccgccttc ttcaataacc gcgccgggtc tggcaccaaa ggcaatcccg
    11101 cgcagcacca cattaagacg cgtttctgcc acaggcacag gttcgggttg ttttaccggc
    11161 gtggcgacgg gctgatattt gccaaaccag ttttgctggc tgattaattg cacgtcattt
    11221 ttatcgaacg tttttgcatc cgcacggggc ggtttattcg ccgatgggga aacagccgta
    11281 tattccgcag aaaaggagaa atagcgccag agtgaatgcg ccacttttgc agaaataata
    11341 agcagcatca gccagaacat cccgcgtaca atttttcgca gatgctcttt gtctgtatta
    11401 agagattgtc tctggttaag agtgtgacga atttttgtca gccattgaat gagataaatt
    11461 cgtgcgtcac gaaaaacaac ccgcgccaat ttatatctac ccgacgttat gctttgacta
    11521 ttccacaggt ggtacgatcc agtttccgct gatactggtt acctgtacgt gtattattaa
    11581 tcgttatgct gtaattaatt ccctcagtga gcattaattt cactgttggg tcagcgcaca
    11641 tttgtcgttg atagctgatt aaaaaggcgt ctggcgtctg cgtggtctga gtacccgctt
    11701 cgctgataat ggtcattttt accgtcgtgc cgctactttg tgccagcact aaggtatatc
    11761 ccgcagattt aatcggcagg ttttggctga tattttgcgc ctgttttttc gccagcaaac
    11821 tggcgttttc attatggctg gcacagccac ttaataatcc tgtaacgctc aacaatagcg
    11881 atattaatac cctccctggc atctgtttta tcgacatggt aaataatctc taaggttatt
    11941 aataagagtt aaaatgtcac tttgataatg acgtggttat cattaaaaca atgcctgtag
    12001 ataaagtgtt gctataccgc ccagacttaa acacggtcca aaaggcagtg tggttgatcc
    12061 tttttttata ataacggcat atatcaggcc gcagcatgag gcgattaaag caacattggg
    12121 cagtgacaac ggccccaccc aactacctaa cgcagcgaac aataatacat cgcccatgcc
    12181 taatgcttct ttacgcagaa ctattccggc tatccagcgc agagagtaaa aagtgataaa
    12241 ccccaccagg acgccggtga ctgcatcttg tagcgtgagc gggctctgct gcgcccatgc
    12301 cgcaattagt cccgtccaca atacgccctg agtaaaaaca tcgggcagcc attggtgatc
    12361 gaggtcaatg acgctcgcgg caatcagcca ggcggataat atcatcaccg ccagcgccca
    12421 tccactttcc ggccagacca gactcgccag caaaaaagcg agtgctgtca ataactccac
    12481 cagcggataa cgcttgctga ttttcgcctg acagtcgcgg cagcgccctt tgagcatcag
    12541 ccaggagagc agcggaatat tgtcacgtat ccggatggtc tgctgacaat gtgggcagtg
    12601 cgaacgcggt agcgcaaggc ttatttttga ctgcgcactc ggcatttcac catgaaactc
    12661 cgccatttgt tggcgcagca tgatggggta acgccaaatc accacattca aaaaactgcc
    12721 gataatcaat cctccgacgg tagccagtat gggcatcgcc gcggggtatt gctgaaaaac
    12781 atcaaaaagc atggttaaag gttgtttgtt gtaacttgcc ggatgcgggc ctgcaggtgt
    12841 atgccatatg gctttcctcc aggcccaata agctggcgca acccggtgcg ccttatttca
    12901 tgccggatgc ggcgtgaacg ccttatccgg gttattcagc aggcatcgaa tactcggtaa
    12961 ccttattaat ggtttccggc ccttgcttcg gtttcggcag attgagtgac gccagcgtgt
    13021 tgtacgccga ctggctcaca ccgccctcga agctcatctc gctcgccccc ggcagctggt
    13081 aagcattcgc acccggattc catttcttaa agaactccga aagatccgtc tgagcgaccc
    13141 aggatgcaca cagcatcagc gtgtcagcag cgttaccgtt ggattcagca cagtaattct
    13201 tgccaccaaa ggtcttgtca ccaacgtcat cgccgcgcgc tttacggtgc atcaactgga
    13261 acaggttcca gcctttcatc ccttcacgct cgctgtaaaa ctctggcaga ggagtgccat
    13321 ctggatacca tttcttgata tcaaagtttt tctctgccca ttccttcagc tgcgcgtaca
    13381 tcagcagacg gtcacccgca ccgccgcgtg cccatgcctg gttgttgctc tcctccagat
    13441 attccggtgc gacggtaata tcgtcagcga cacggttcat cttgccgaga tagcgatcct
    13501 gcatgtacag cgccagcacg ttgttcgcga cttcagttgc acccggtaca gtcagcggtg
    13561 tttctgcggc gttgtgccct acttcgtgcc agatcaacca gtcgttcagc ggcgtcgtcg
    13621 gcagcgtggt gctgttcgtc gagaagctgc tgttcattac cggataaccc gagtgcgcat
    13681 caccgatgga gatctgcaca tcgtttgtga aacgatgttt atgtcccgtc aatgctttat
    13741 aggtaaacat ccggtgctta ccgctcgttt catcacgacc gtagaagtca ttcatcgatc
    13801 tggcaaaggt gtccagatcg ttagcgaatt gctccagtcc gccagtgtaa ttgctggcat
    13861 tcaggttctt cttcggtgtg gtatagacga aagcgtctga ttccagctca cccagcggag
    13921 ccggtgagtt cagatcgttt ttccatgcgc cgtctttata gaacggtgct tttaccacgc
    13981 cagtaaaggt gaagctggca gattcattgg tagagctatt gcccttgata taaatcaggc
    14041 caccgtaagg caccttgaac ttcaccgtac cgctagcgtc cagagagtac gttttagtca
    14101 ctcttggcgg acggttcagc gcaacttcat gcttctcacg tccggtcagg tcgtcagcca
    14161 gcgccacggt gacggtcaca ggaacgttcg cattggactt aatggtgacc tctttctgag
    14221 ccggtgccca caggccagtt gactgcatgt tacctgcaaa ccatttggtc ggattcgagt
    14281 acaggctgat ggtttcagta acgttctgtc cctcttccga tactgctccc ggatacttct
    14341 cgacatcaac tttgatgttc agatcccacc aggaacggcc cagcatcagg cgtgtcagcg
    14401 gtttttccat atagttgagc gggtagctcg ggttcatcat gcccgctttg ctgctaccgt
    14461 caccgtagat catgttgtta tcgaccagcg attttttcag atctgcagaa cacttggtgc
    14521 cgcctgcata ggcatcattg gcgtagcagt tcaggaactc ggtgaacgtt ttaaagccca
    14581 gctcgtcatt tttgccttct tcataacgat agctcgtatc gttccacagc cagaccgaca
    14641 tgttctggta cagacgttcc agatcgacgc tgctcagacg ctcacctttg cgaccattgg
    14701 tccggaagta gagctcatgc tgatacagac gctgaatgtt ggtgcctaaa tccgcagcct
    14761 gcaccatcgc ttttgcagtg tcggcgttaa ggcttagttg cgtatactgt ggaacataca
    14821 tgccaccagt aaccggaacc cccgtgccag gacgatattc cagacagttg acctcatagt
    14881 gatatgccgg attagtacac tctttcagcc ccgggaacgc ggcgaaaatt ttctccttcg
    14941 cagccttcag agaatcctct gttttatgat cggcctcatc aataaaggca taacgcgttt
    15001 cctgtttgcc atctacatct tccagccagc tggcaacttc cagcttcggt ttgtcatcag
    15061 gtttgttttc tacctgatat ttccacttaa cttcccctgt cttactatcg atggtgtacg
    15121 gcagcgcacc atctacggca ggataacgtt catagaccca aatgcccgtt gcgcgctgct
    15181 gacgaacgcg gttcggatac ccttgcggat cgttatttac taccgacttg ttcagtgcca
    15241 tcgacagacc tgcggcatcc aacagacgca caaaaccaga cgcgctctct tccttaagat
    15301 tgctcatcac gttttccatg atcagcaccg atccaccttt gttcagatag gcgatcagat
    15361 cggtcacatc ctgctgagtc agcttcggtt tgctggtatc tgcacgcagc gggattgcat
    15421 aaggatcgtt acccacctga gtcacatatt caaagccgtt aaggatcagc agcggcattt
    15481 cctgcggatc gagatcgcca tagctactta aatgctcaac agagatgccc gcaaaatccg
    15541 gatggaagtc gaacgcagcg ctgtttcctg taacctgacc atgacgttta aaatagacag
    15601 tatccaggtt ggtgcctacg gtcatgctgg ctttcgcgtc cggcgtccat ttatcgtcgg
    15661 acagatagcg cagcacgttc tccatgaagt tcttcatgtc atccgggtcg ctgttgagcg
    15721 tacactgccc atctttatta acgcccccgt tccagctgta accgttcggg caacgcaaaa
    15781 tgctgttgta gtgtgggtta ccgataacca tcagtttgcc ctcaccgact tgccccagcg
    15841 aaataaacgg caggttgaaa gtggcggtat cgcgcgtaac gttttccggc tcaacaagag
    15901 aaggcgcttc cgtaatgtac gccagctcat ttttatccca ggcgcgtttt tcgccaaacg
    15961 ccagccagta gtttttatca ttacgcgcca tcagaatcgg gaaggccgcg ttggagatat
    16021 tcaccaccgc ctgaccgcgc gcattacccg tgctgccata gaagttggta gagtcatgga
    16081 atacatggaa cttgctgaca gatttgtagt tcgtatccac gccccacagc ttgttgataa
    16141 cgccctgaat ctggccgtca ttaacattgc gcgtcgtcag cgagaaccag cgagcctcgt
    16201 tacaaccgtc ggttttcgca caaatcgcgg tatcgatctc tttggcctga cccgtcttaa
    16261 actgctcgat aaattcgtta ggcagattaa cgacttgctc accttccccc agcgtcgcac
    16321 cattggataa cgagagattg ataatctcgt taatcacgtt gggatattca gcaaaaacct
    16381 tgcgcacttc atccggaact acacgcgtgt gattttgtcc agccttcgaa tagcggtgaa
    16441 tcaactgatc gatatttgcc ccgcgaactt catcacccag ttcagtcaat gcaatagtcg
    16501 acttgttacc acgcacagaa ccaagctcaa aagtgtcgat gccaaaggag atggtttccc
    16561 cccaactaaa ggaaaattcc ccgttttctc cggttacacc acggccggaa ttggtgtaat
    16621 agttgacacc aacaacacca tccccctgac tatcgaccag tcgaccttca gagagaatga
    16681 tttcagtggg ttgatactga taaaactgtt ccgcgttagc cgacacgaag gaagcgttta
    16741 gatccggttt tgttcccgga gtggtggcgg gcacgaccgg tgaagtatga gtggatggcg
    16801 ctttatcggt cgcggcattg ttttccacct cttcattgac cagctttttg aattcttccg
    16861 gtgccagatc gatttgctta tacagcgagt cgaaacgttt actctcgatc accgaggaga
    16921 actccaggca cacctgttct gtattcgccg ggcaactgtt catggaggtg accagcgaga
    16981 gcgcattgct tttcttgttg tcggaacccg ccagttcttg cgcgtcctca agactaaacg
    17041 acactttttc aaccgcacgc aggctacgcg cagcttctga ctgggtgtcg aaggtagcaa
    17101 ttgtcgtgtt gttccctgcc acacaggtga ctttgtcgcc tggtgtaaag gtaaagccat
    17161 cgctggattc atcattacag gtgatatcac cagttacccg caggcttccg cccagagtca
    17221 gataacccgt tttcgtagga acaggttctg gctccggttc tggtgttggc tcaggatcag
    17281 gtgtcggttc cggcgtaggt tctgggtccg gcgttggctc aggcgtcggc tccgggtttg
    17341 gtgttggatc aggtttcact tccggcaaag accctgttcc agaatctaca ggcggcgtat
    17401 cggaggaaga tccggagcca ccgccatcac aaccggctaa cagggttgca ctc
//
LOCUS       DAZGVQ010000110        17348 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000110 DAZGVQ010000000
VERSION     DAZGVQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.111"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..113)
                     /gene="ylcJ"
                     /locus_tag="ACHLAG_003497"
     CDS             complement(<1..113)
                     /gene="ylcJ"
                     /locus_tag="ACHLAG_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YlcJ"
                     /protein_id="HFP0656661.1"
                     /translation="MSLVLCFLLMSLFFMYSFVLSRLWRKKIAIRLLLYIQ"
     gene            complement(161..493)
                     /gene="emrE"
                     /locus_tag="ACHLAG_003498"
     CDS             complement(161..493)
                     /gene="emrE"
                     /locus_tag="ACHLAG_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFP0656662.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGLFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSTPH"
     gene            complement(561..863)
                     /locus_tag="ACHLAG_003499"
     CDS             complement(561..863)
                     /locus_tag="ACHLAG_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFP0656663.1"
                     /translation="MTGKEAIIHYLGTHKSFCAQDVAAVTGATVTSINQAAAKMARAG
                     ILVVDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAAMKRVLRVYKRTSMGTQ"
     gene            complement(860..1561)
                     /locus_tag="ACHLAG_003500"
     CDS             complement(860..1561)
                     /locus_tag="ACHLAG_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP0656664.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVNAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(1558..2577)
                     /locus_tag="ACHLAG_003501"
     CDS             complement(1558..2577)
                     /locus_tag="ACHLAG_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP0656665.1"
                     /translation="MTGSEMAKVGLREQNRLSGANRNTLIAGGIMANTAEIFNFPVPD
                     AAQKERRVADLDDGYTRIANELLEAVMLAGLTQHQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPESGKKSLPKSGKGVYPNQVNTKDKLTKDNIKPFSSENSGESSDQPENDFPVEK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTAGKSKLDLTNTDWI
                     YGVDL"
     gene            complement(2574..3113)
                     /locus_tag="ACHLAG_003502"
     CDS             complement(2574..3113)
                     /locus_tag="ACHLAG_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
                     /protein_id="HFP0656666.1"
                     /translation="MQPLTYQQTSGFSPTAVINRSQTKQAPGHEKIRDAVRAWSAADN
                     QDVVAALIVNEYREQGGGTIDFPDDVSRARQKLFRFLDNKFDSEKYRNNVRELTPAIL
                     AVLPLEYRGYLVEQDSFMARLAEMEKELSEAKQAVILNAPRHQKLKEISEGIVSMFRV
                     DPDLAGPLMAMVTTMLGAI"
     gene            complement(3144..3371)
                     /locus_tag="ACHLAG_003503"
     CDS             complement(3144..3371)
                     /locus_tag="ACHLAG_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0656667.1"
                     /translation="MTNQTIQLAISITGSQKRLADLCGVAQPTVWRWLHGGGIDARYV
                     MKIVSATGGKIKPADIRPDLAPLFNASNSAA"
     gene            3482..4174
                     /locus_tag="ACHLAG_003504"
     CDS             3482..4174
                     /locus_tag="ACHLAG_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001337214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP0656668.1"
                     /translation="MKKETLADRLNLAMEQSGMSQGALAKASGVAQPTIWRLTSGNAR
                     GSTKIVEIANALGVRTEWLSSGIGPMRNDGQQLGKPTANHPKYFKIDVLDIEVSAGPG
                     VINREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITV
                     KSFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKV
                     IGSMPQTYRKHG"
     gene            4329..5189
                     /locus_tag="ACHLAG_003505"
     CDS             4329..5189
                     /locus_tag="ACHLAG_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001750114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656669.1"
                     /translation="MNVRDLAPALLSLSDALEEAGKTLYGNKTVVSVKVNASFKAGSF
                     GIDLVASSTSWFKQAVDFLSGDSATAAANLIAFIGLCPGSKEKICKGLIQLIKWIGPR
                     KIKKLHNLPDSNIEVFVDDESEIYDSNVIELYKNIKLRSSLQEFISKPLEQEGIDSFA
                     STVDDGLTFMTINKQEAHYFKVNLPAESIISEYTVEKALQIKNISFNEGSRWRFSDGA
                     SSFLAEIKDQKFISDIDNNTLNFSKGDMLLVDLKVTQYMIGDAIKTIFEIEHVKKQLN
                     PQRQIDLPFE"
     gene            5671..5877
                     /locus_tag="ACHLAG_003506"
     CDS             5671..5877
                     /locus_tag="ACHLAG_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFP0656670.1"
                     /translation="MVHQHYGTQTVNRGAVRPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNR"
     gene            5953..6249
                     /locus_tag="ACHLAG_003507"
     CDS             5953..6249
                     /locus_tag="ACHLAG_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP0656671.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVGFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            6255..7040
                     /gene="bet"
                     /locus_tag="ACHLAG_003508"
     CDS             6255..7040
                     /gene="bet"
                     /locus_tag="ACHLAG_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFP0656672.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            7037..7717
                     /locus_tag="ACHLAG_003509"
     CDS             7037..7717
                     /locus_tag="ACHLAG_003509"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP0656673.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVMRKDAWYFANYDPRMKREGLHYVVIERNEKYMANFDEMVPEFIEKMDVALAEIG
                     FVFGEQWR"
     gene            7714..7875
                     /locus_tag="ACHLAG_003510"
     CDS             7714..7875
                     /locus_tag="ACHLAG_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HFP0656674.1"
                     /translation="MKHPHDNIRVGAITFIYSVTKRGWVFPGLSVIRNPLKAQRLAEA
                     INNKRGLYD"
     gene            7868..8425
                     /locus_tag="ACHLAG_003511"
     CDS             7868..8425
                     /locus_tag="ACHLAG_003511"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFP0656675.1"
                     /translation="MTDFTGSNTPAEHRDSWRTPPEIFAALNAEFVFQLDAAASEKNR
                     LCRLFISQEQNTLTTSWPEAMGYASGYVWLNPPYSNISPFVKKAATENKFSSVGCVML
                     LPADTSVGWFHEAIQTASEVRFITAGRLAFINPLTEKPVSGNNKGSMLIIWHPYPRTH
                     CHFTTVDRGELMAFGSRILARREAA"
     gene            8502..8717
                     /locus_tag="ACHLAG_003512"
     CDS             8502..8717
                     /locus_tag="ACHLAG_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP0656676.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            8816..9034
                     /locus_tag="ACHLAG_003513"
     CDS             8816..9034
                     /locus_tag="ACHLAG_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP0656677.1"
                     /translation="MADIFDSASEIEELQRNTAIKMRRQNYQTVSATHCCECGDPIDE
                     RRRLAVQRCRTCASCQEEIELKNKQWGL"
     gene            9082..9315
                     /locus_tag="ACHLAG_003514"
     CDS             9082..9315
                     /locus_tag="ACHLAG_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0656678.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMR"
     gene            9332..9427
                     /locus_tag="ACHLAG_003515"
                     /pseudo
     CDS             9332..9427
                     /locus_tag="ACHLAG_003515"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            9559..10723
                     /gene="intD"
                     /locus_tag="ACHLAG_003516"
                     /pseudo
     CDS             9559..10723
                     /gene="intD"
                     /locus_tag="ACHLAG_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(10739..10815)
                     /locus_tag="ACHLAG_003517"
     tRNA            complement(10739..10815)
                     /locus_tag="ACHLAG_003517"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10779..10781),aa:Arg,seq:tct)
     gene            11058..11690
                     /gene="fimZ"
                     /locus_tag="ACHLAG_003518"
     CDS             11058..11690
                     /gene="fimZ"
                     /locus_tag="ACHLAG_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFP0656679.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(11693..12208)
                     /gene="sfmF"
                     /locus_tag="ACHLAG_003519"
     CDS             complement(11693..12208)
                     /gene="sfmF"
                     /locus_tag="ACHLAG_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFP0656680.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGAASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(12219..13226)
                     /gene="fimH"
                     /locus_tag="ACHLAG_003520"
     CDS             complement(12219..13226)
                     /gene="fimH"
                     /locus_tag="ACHLAG_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFP0656681.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(13239..15848)
                     /gene="sfmD"
                     /locus_tag="ACHLAG_003521"
     CDS             complement(13239..15848)
                     /gene="sfmD"
                     /locus_tag="ACHLAG_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFP0656682.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMS
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGIRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDELIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(15879..16571)
                     /gene="fimC"
                     /locus_tag="ACHLAG_003522"
     CDS             complement(15879..16571)
                     /gene="fimC"
                     /locus_tag="ACHLAG_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP0656683.1"
                     /translation="MMTKIKLLMLIIFYLIISASTHAAGGIALGATRIIYPADAQQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(16791..17333)
                     /gene="fimA"
                     /locus_tag="ACHLAG_003523"
                     /pseudo
     CDS             complement(16791..17333)
                     /gene="fimA"
                     /locus_tag="ACHLAG_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
BASE COUNT         4844 a         4138 c         3936 g         4430 t
ORIGIN      
        1 tcttgaatat acaacaataa tcgtattgca attttcttac gccataatct tgaaagcaca
       61 aaagaataca taaaaaataa agacattaac aaaaagcata aaacgaggct catataaata
      121 taagagcctc catattttag tcgtttagaa acaaattatt ttaatgtggt gtgcttcgtg
      181 acaataaatt aataaccaac acaccggcac aaatcaacat catgcctata atggctggca
      241 ggtccagccg ttggccgaaa agtccccatg acagtaagct aatcaggaca ataccgactc
      301 ctgaccagat agcataagca atccctgtag gaatataagc cagcgtctga gctaataacc
      361 agaatgatgc acaataacaa ataattgtac caacagatgg ccataaccgt gtaaaacctt
      421 ctgaaaactt cattaaggtt gtaccaatga cctctgcaag tattgcacca ccaagataaa
      481 tataagggtt catagcatat tctttcctgt tcaaactgga gagaattgta ctacagtttg
      541 aactcaactc acctgtttca tcattgtgtt cccattgatg ttcttttata taccctcagt
      601 acccgtttca tcgcggcact ctggcgacac tccttaaaaa tcagattcgt gctcaccttt
      661 ccttcccgtt cttctctggt agcgaaccgg taatacaccg ttcgccagac cttaccatca
      721 acgaccagga ttcctgcccg cgccatttta gccgcagcct gatttatgct ggttacggtt
      781 gcgcctgtta ccgcggcaac gtcctgtgca cagaagctct tatgcgtccc caggtaatga
      841 ataattgcct ctttgcccgt catacacttg ctcctttcag tccgaactta gctttaattt
      901 ctgcgatctt cgccagagcc tgtgcacgat ttagaggtct accgcccata acaggaagtt
      961 gttttactgg ttcaggtatc gtctcaccac ggttaattcg cgctgtcata caggtcagtt
     1021 catcggcagc cttgcgccgt aattccgcgt cagtcagcgc attggcccgc atgttctggt
     1081 acaagttggt aaccaaccag taatgcgcgt tcgatttcca cggataagac tctgcatccg
     1141 gatacaggcc acgcttccgg caatactcgt aaaccatatc aaccagctcg ctgacgtttg
     1201 gcagcccggc gttaacagat gcttcttccc ggcaccaggc gacaaactgc ccgggtgatg
     1261 gcaggaatgg tcgattctgc cgacgggcta cgcgcattcc tgcgttaacc tgttccattg
     1321 tggtgatccc gttttcccgg aaagccagaa cccactggcg gcggatttcg ttcagttcat
     1381 tctggtcacg gttagccagg ctcgccggga aagttgccag taactggctg aacacaccgt
     1441 tgatgatctg cgctacctgc tgtacctgcg gcttttcgtc gtactgttcc ggcatgttgt
     1501 tggcgatccg gcgcatctgc tcacggtcaa agttaaccat ctgtgcggcg atgtttttca
     1561 taaatccacc ccgtaaatcc agtcagtgtt tgtcaggtcg agttttgatt ttccggctgt
     1621 cacgccagcc tgttgcttgt tacggttgat ttcgagttgg gtccacttgt cgcggagttt
     1681 ggccggactc agcacgttac cggaccagaa gttgtcctgg catgcccagc ggaacagcac
     1741 gcacatgtcg cggtggttac gtccgtcacg ttcacgcatc aggcggatat cgttagccca
     1801 ccctgcaaaa ttcggttttc tggctgaggg cgcgatggtc ttcaccatgt caaacatcca
     1861 ctctgcggcg gtcaggtctt ctgctgtccc ccacttgctg ccgctctgaa ttgcagcatc
     1921 cggtttctcc acaggaaagt cgttttctgg ctggtcggag gattcgccag aattctcgga
     1981 cgaaaaaggt tttatattgt cttttgttag tttgtctttt gtgtttacct gattcgggta
     2041 aacgccttta cctgatttgg gtaaactttt cttacctgat tcaggtaaat ttacctcttt
     2101 caggtaaact ttatttttct tacctgattc gggtaatgtt gaccattcac tgaccacatt
     2161 attaatgccg atattccgcc cactctgaat aaaaatccca cgctttacca gaacactttt
     2221 tgcagcagaa cacttgtgcg gcaatatccc ggtcaactcg gaaagttgct cgttgctcac
     2281 ccaatccagt tttttattaa agccatatgt tttgcgcatg acagccagga agaccagaag
     2341 ctggtgctgt gttaatccgg ccagcatcac agcttccagc aactcatttg caatgcgcgt
     2401 ataaccatca tcgagatctg ccacgcgccg ctccttttgt gccgcatccg gcactggaaa
     2461 attgaatatc tcagcagtgt ttgccataat tcctcccgca atgagtgtgt tacgatttgc
     2521 acctgaaagt cggttctgtt cccgcagacc gactttcgcc atttctgaac ctgtcatatt
     2581 gcccccagca tggtggtgac catcgccatc agtggaccag ccagatccgg gtccacacga
     2641 aacatcgaca caataccttc actaatttcc ttcagtttct ggtggcgtgg tgcgttgaga
     2701 atgacagcct gttttgcctc actgagttcc ttttccattt cagccaacct agccataaag
     2761 ctatcctgct caaccaggta accacgatat tccagcggta gtaccgccag aattgccggg
     2821 gtcagttcac gcacgttatt tcggtatttt tcagaatcga atttgttatc gaggaagcgg
     2881 aacagcttct ggcgtgcacg gctgacatca tcagggaaat cgatggtgcc gccaccctgc
     2941 tcccgatact cattcacaat gagtgcggca acaacatcct gattatctgc agccgaccag
     3001 gcgcggacgg catcacggat tttttcgtgg cctggagctt gttttgtttg agaacgattt
     3061 atcaccgcag tcgggctaaa tccgctagtc tgttggtatg taagtggttg catagtcatt
     3121 gccttatcag ttaacgccgc agtttaggcg gcagaattac tcgcgttaaa caatggtgcg
     3181 aggtcgggac gaatatctgc tggtttaatc tttccaccag tggctgagac aattttcatt
     3241 acatagcggg catcaattcc gccaccgtgt agccaacgcc aaacagtggg ttgggctaca
     3301 ccgcatagat ctgccagtcg tttttgacta cctgtaatac tgattgcgag ttgaatggtt
     3361 tgatttgtca ttatcaattc ctattggtat tggaatgaac aaataatagc aatgcgtatt
     3421 aatcataaca atagcaaaac gtgttttgac catcaatacg caagcgtata aattaaaact
     3481 tatgaaaaaa gaaactcttg ctgatcgctt aaacctagcg atggaacaat ctggaatgtc
     3541 tcaaggcgct cttgcaaagg cgtctggcgt agctcaaccc acaatctgga gactgacaag
     3601 cggcaacgcg cgcggctcaa caaaaattgt tgaaatagct aatgcattgg gtgttcgaac
     3661 agagtggctc tcatcaggca taggcccgat gagaaatgac ggtcaacaat tagggaagcc
     3721 tactgccaac catcccaaat acttcaagat tgacgttctt gatatagaag tgagtgccgg
     3781 gccgggagtc atcaaccgtg agtttgtaga agttctacgc tcggttgagt actcgtttga
     3841 cgatgctcgt cacatgttcg atggcaggaa ggcagaaaat atccgcatca ttaacgtacg
     3901 cggtgacagc atgtcaggaa cgatcgaacc aggtgatctt ctgttcgttg atatcactgt
     3961 taaatctttc gacggtgatg gtatctatgc gtttctgtac gacgacacag cccatgtaaa
     4021 gcgtctgcaa atgatgaagg ataagctgct ggttatctct gataacaaga gctactcgcc
     4081 gtgggacccg atcgagaaag acgagatgaa ccgggtgttc atcttcggta aggttattgg
     4141 gagcatgccg cagacgtata ggaagcatgg gtagccatac aatagaagtt ttaagattta
     4201 agacaatatc gtaaattaac tgtatatgtg atcaggtgaa tgccataacg gatgcgggca
     4261 acaattcgga aaccaatgat atgaagttca ggatagtata cgacggtccg gcactggaaa
     4321 cgcatgaaat gaacgtgcga gaccttgctc ctgcccttct atcactatca gatgcattag
     4381 aggaagccgg taaaactctc tacggaaaca agactgttgt ttctgtaaaa gtcaatgcat
     4441 catttaaagc tggctcattt ggaatagatc tggttgcctc atctacatct tggttcaaac
     4501 aggctgttga ttttttatcc ggcgattcag caacagccgc tgcgaatttg attgctttta
     4561 ttggactctg tcctggttct aaagaaaaaa tatgcaaagg cctaattcaa ctgataaaat
     4621 ggataggccc aaggaaaata aaaaaattac acaatttgcc tgatagcaac atcgaagtct
     4681 ttgttgatga cgaaagcgag atctacgaca gtaacgttat tgaactttat aaaaatatta
     4741 aacttcgctc ttcattacaa gaatttataa gtaagccatt agagcaggaa ggcattgaca
     4801 gctttgcctc tactgtcgat gatggtttga cattcatgac gatcaacaag caagaagcac
     4861 attatttcaa agtcaatcta ccagcagaat caataatttc agagtacaca gtagagaaag
     4921 cccttcaaat taaaaatatt tccttcaatg aaggaagccg gtggcggttt tctgatggtg
     4981 ccagtagctt tttggctgaa ataaaagacc agaaattcat cagcgatatt gataacaaca
     5041 ccttaaattt ttctaaaggt gatatgttac ttgtagattt aaaagtaact caatatatga
     5101 tcggagatgc cataaaaacc atattcgaaa ttgagcatgt aaaaaaacag ttgaatcctc
     5161 aaaggcaaat agatcttcca ttcgaataaa gatacccggc caccgtgccg ggttttcttt
     5221 tgtcccctcc cctcatcaca caaaccgttc gaaaaaccac cacaacctcc cttcagttat
     5281 cgctatgcga tgcaagtcac aaaattaatt ctttttgcta tcaaacagtt aatatcaaaa
     5341 cacatcaatc aatagcaata gctattgata ccaccaatag caatagctat tatcaccatg
     5401 tcgcaacaac acaacgatac ggcaaccacc tgattcaccg ttgcgatgac cgcttagatc
     5461 cgcagcttga atttcggcag gctccgggga gtgcgagggg tgaagcggac gcgtgaacgt
     5521 cggtgtgacc agctgaaatc aacacaacac tttatacctc agtcgcttca acgaggcggc
     5581 ttagttatga caaccggcgg ccatccaccg cctgaatacg cgcagaagtc tctatatgtt
     5641 cagcagccca gcttacgggc aggagttttt atggttcatc aacattacgg aactcagacc
     5701 gttaatcgcg gtgccgtccg gccaggtatg ctggttaaac acaaagatgg tacctggaca
     5761 gcatcagcta atttacgcgg acggctttat ctgcatcgcg gcatcgagcg cacttatacc
     5821 cgtgatttgc tcgtggaagt ttttctcgac ggacgcggta acggcctgaa tcgctaatcc
     5881 cctttcctgt tttcctaatc agcctggcat ttcgcgggcg atattttcac agccattttc
     5941 aggagttcag ccatgaacgc ttattacatt caggatcgtc ttgaggctca gagctgggcg
     6001 cgtcactacc agcagatcgc ccgtgaagag aaagaggcag aactggcaga cgacatggaa
     6061 aaaggcctgc cccagcacct gtttgaatcg ctatgcatcg atcatttgca acgccacggg
     6121 gccagcaaaa aagccattac ccgtgcgttt gatgacgatg ttgggtttca ggagcgcatg
     6181 gcagaacaca tccggtacat ggttgaaacc attgctcacc accaggttga tattgattca
     6241 gaggtataaa acggatgagt acagcactcg caacgctggc agggaagctg gctgaacgtg
     6301 tcggcatgga ttctgtcgac ccacaggaac tgatcaccac tcttcgccag acggcattta
     6361 aaggtgatgc cagcgatgcg cagttcatcg cattgttgat cgtcgccaac cagtacggcc
     6421 ttaatccgtg gacgaaagaa atttacgcct tccctgataa gcagaacggc atcgttccgg
     6481 tggtgggcgt tgatggctgg tcccgcatca tcaatgaaaa ccagcagttt gacggcatgg
     6541 actttgagca ggacaatgaa tcctgtacat gccggattta ccgcaaggac cgtaatcatc
     6601 cgatctgcgt taccgaatgg atggatgaat gccgccgcga accattcaaa actcgcgaag
     6661 gcagagaaat cacggggccg tggcagtcgc atcccaaacg gatgttacgg cataaagcca
     6721 tgattcagtg tgcccgtctg gccttcggat ttgctggtat ctatgacaag gatgaagccg
     6781 agcgcattgt cgaaaatact gcatacacgg cagaacgtca gccagaacgc gacatcactc
     6841 cggttaacga tgaaaccatg caggagatta acactctgct gatcgccctg gataaaacat
     6901 gggatgacga cttattgccg ctctgttccc agatatttcg ccgcgacatt cgtgcatcgt
     6961 cagaactgac acaggccgaa gcagtgaaag ctcttggatt cctgaaacag aaagccactg
     7021 aacagaaggt ggcagcatga caccagacat tatcctgcag cgtaccggga tcgacgtgag
     7081 agctgtcgaa cagggggatg atgcatggca caaattacgg ctcggcgtca tcaccgcttc
     7141 agaagttcac aacgtgatag caaagccccg ctcaggaaag aagtggcctg acatgaaaat
     7201 gtcctacttc cacaccctgc tggctgaggt ttgcaccggt gtggctccgg aagttaatgc
     7261 taaggcgctg gcctggggaa aacagtacga gaacgacgcc agaaccctgt ttgaattcac
     7321 ttccggcgtg aatgttactg aatccccgat catctatcgc gacgaaagta tgcgcaccgc
     7381 ctgctctccc gatggtttat gcagtgacgg caacggcctt gaactgaaat gcccgtttac
     7441 ctcccgggat ttcatgaagt tccggctcgg tggtttcgag gccataaaat cggcttacat
     7501 ggcccaggtg cagtacagca tgtgggtgat gcgaaaagat gcctggtact ttgccaacta
     7561 tgacccgcgt atgaagcgtg aaggcctgca ttatgtcgtg attgagcgga atgaaaagta
     7621 catggcgaat tttgacgaga tggtgccgga gttcatcgaa aaaatggacg tggcactggc
     7681 tgaaattggt tttgtatttg gggagcaatg gcgatgaagc atcctcacga taatatccgg
     7741 gtaggcgcga tcactttcat ctactccgtt acaaagcgag gctgggtatt tcccggcctt
     7801 tctgttatca gaaatccact gaaagcacag cggctggctg aggcgataaa taataaacgg
     7861 gggctgtatg actgatttca ccggaagcaa tactcctgcc gaacatcgcg acagctggcg
     7921 cacaccacca gagatttttg ctgcgcttaa tgcagagttc gtttttcaac ttgatgctgc
     7981 cgccagcgaa aaaaaccgac tatgtcggct ttttatctca caggagcaga acacattaac
     8041 cacttcatgg cctgaagcaa tgggatatgc ctctggttat gtctggttga atccaccata
     8101 cagcaatatt tccccttttg tgaaaaaggc agccactgaa aacaaattca gtagtgtggg
     8161 atgtgtaatg ttattgcctg ctgacacatc tgtcggatgg tttcatgaag cgatacaaac
     8221 cgccagtgag gtcagattca tcacggcagg acgactggca tttattaacc cactcactga
     8281 gaaacccgtc agtggaaata ataaaggctc gatgctcatt atctggcacc catacccccg
     8341 tacacactgc cactttacga ccgttgatcg tggagagttg atggcgttcg gctcaaggat
     8401 tcttgcccgt cgggaggctg catgacaacc acggaatgca tttttctggc agcgggcttc
     8461 atattctgtg tgcttatgct tgccgacatg ggacttgttc aatgacacct cagcaggaaa
     8521 acgcccttcg cagcattgcc cgtcaggcta attctgaaat caaaaaagcc agacagcagt
     8581 ttccggataa aaacgtcgat gacatttgcc gtagcgtact taagaagcac cgcgaaacgg
     8641 taacgctgat gggattcaca ccgactcatt taagcctggc gatcggcatg ttaaacggcg
     8701 tctttaagga acggtgaaca tgaaaagcaa aatcatcagg gagctacagg ctcctttttt
     8761 attgttcgca ttcatcctca agcgtattaa ccaacaattc agggattaat ggaagatggc
     8821 agacatcttt gattcagcat cagaaatcga agaattacag cgcaatacag caataaaaat
     8881 gcgtcgtcag aactaccaga ctgtatccgc aactcattgt tgtgagtgtg gcgatccgat
     8941 agatgagcga agacgcctgg ctgttcagcg ttgtcggact tgtgcaagtt gccaggagga
     9001 gatcgaactt aagaacaaac aatggggact gtgatggcct caaagcagca aatttcaaca
     9061 tcgtccaact gaggtgtaaa aatgttcaga atcatttttc ctaacacctg gtacgtcgac
     9121 caccacggca ctccctgcaa aatcctgcgt tctacccaca acaaagttca ctacatccga
     9181 aaaggcagaa catgtatcgc cagcatgttc cgctttaatc atgactttga acctgtgaat
     9241 aaagctgatg cagatcggat agcagaagag atcgaaacgg cagaacacat taagaagtta
     9301 cgtgacatgc gttgaaaaag cagaggtaac catggaatca tacagcctca cactcgatga
     9361 ggcctgtcag tttcttaaga tatccagacc aaccgccacc aactggatac gaacaggccg
     9421 cctacaggca acacgtaaag atccaaccaa gccaaaatct ccttacctca caacacggca
     9481 agcctgcatt gcggcgcttc agtctccgct gcatactgtc caggtgagcg cgggtgatgg
     9541 cataacagag gaaagaaaat gtcactcttc cgcagaaatg aaatatggta tgcctcgtat
     9601 tcgctcccgg gcgggaaacg aattaaggaa tctcttggca caaaggacaa gcggcaagct
     9661 caggagttgc acgacaagcg aaaagcagaa ctctggcgag tagaaaagct aggggattta
     9721 cctgatgtca cttttgaaga ggcctgccta agatggcttg aggaaaaagc tgataaaaaa
     9781 tctctcgatt cagataaaag ccggattgag ttctggcttg aacattttga gggtataagg
     9841 cttaaagata tctcggaggc aaagatttac tctgctgtaa gcagaatgca taacagaaag
     9901 acgaaagaaa tatggaaaca gaaagttcag gccgccatca ggaaaggtaa agaaccgcct
     9961 gtttatgaac caaagccagt atcaactcag acaaaggcaa agcatcttgc catgataaag
    10021 gccattctcc gtgctgcaga acgcgactgg aagtggctgg aaaaagcgcc tgtcatcaag
    10081 ataccagcgg tcagaaacaa gcgagtcaga tggctggaaa aggaggaagc aaaacgcctt
    10141 attgatgagt gccccgaacc actgaaatct gtcgtcaagt ttgcgctggc aactggtctg
    10201 agaaagtcga acatcataaa tctggaatgg caacaaatcg acatgcagcg acgagttgcc
    10261 tgggtgaatc cagaagagag caaatcaaac cgcgccattg gtgtggcgct gaacgatacc
    10321 gcctgtaaag tgttgcgtga tcaaataggc aagcatcaca aatgggtgtt tgtacatacc
    10381 aaggcggcta agcgagcaga tggaacatca acgcctgcgg tcaggaagat gcgcatcgac
    10441 agcaagacat catggctatc agcttgtcgt cgtgcaggaa ttgaagattt ccgtttccat
    10501 gacctcagac acacctgggc aagctggctg attcagtcag gcgtcccatt atcagtgctt
    10561 caggaaatgg gcggatggga gttccataga aatggttcgt aggtatgctc accttgcgcc
    10621 taatcatttg acagagcatg cgaggaaaat agacgacatt tttggtgata atgtcccaaa
    10681 tatgtcccac tctggaatta tggaggatat aaagaaggcg taactgattg aattgtaatg
    10741 gcgcgccctg caggattcga acctgcggcc cacgacttag aaggtcgttg ctctatccaa
    10801 ctgagctaag ggcgcgttga taccgcaatg cggtgtaatc gcgtgaatta tacggtcaac
    10861 ccttgctgag tcaatggctt ttgatctggt tgctgaacaa gtgaacgacc gcgtctgatt
    10921 ttctgattta tttcgctata gcggcaaaca aacacacacc gctgcgcgtc tgaatcaaga
    10981 aaacccgtat tttcatgtat caaagtacaa tttaccgccc taacggaaaa ttgtccgctc
    11041 ctatgagact ggtaactatg aaaccaacgt cggtgatcat tatggatact catcctatca
    11101 tcagaatgtc tattgaagtt ctgttgcaaa aaaacagtga attgcagatt gtcctgaaaa
    11161 cggatgatta ccgcataacc atcgattatc tccgaacccg tcctgttgat ttaatcatta
    11221 tggatataga cttgcccgga acagacggtt ttaccttcct gaaaaggatc aaacaaatcc
    11281 agagcacagt gaaagtgtta tttttatcat cgaaatcaga atgcttttat gctggcagag
    11341 cgatacaagc tggtgctaac ggttttgtca gtaaatgcaa tgatcagaat gatatttttc
    11401 atgccgttca gatgatcctc tccggataca cgttttttcc cagcgaaacg cttaactata
    11461 taaaaagcaa taaatgtagt acgaatagtt caacggtcac tgtgctatct aatcgtgaag
    11521 tgaccatatt acgttatctg gttagcggat tatctaataa agaaattgcc gataagttat
    11581 tacttagcaa taaaacagtt agtgcgcata aatctaatat ttatggcaag ctaggtttgc
    11641 attcaattgt agagcttatc gactacgcca aattatacga attaatataa tattaattat
    11701 aattgatcat aaatatcgca tccgctttcg ccacacctgg ccgaacaccg ctggctaacg
    11761 ctcgataatt ggcaaaaaag tttagtgtga cattgccatt tgcatctact tcctcagtcg
    11821 ggctcgccgc tcccagtgcg agccgggagc gatcgctatt acgtaattcg atggcgacgg
    11881 tttgcgccat tgcgggatca tccagagcca gcaggttggt atcggatgcc ggcgttcccg
    11941 taaataaaat cgcaactgaa cccggaggac atccctccag ccgcaggcta aaagggacga
    12001 gtgccgtggt atcgccagcg ttcagtaatt gtgtcgtagg ccatctgcct aaatctaccg
    12061 tcttatcaat atccgcagtg tttacggtac aggagaaatc aacaacgtta ccgtgcaaat
    12121 tgatattaat cgttcccaaa gggtcagctg cccatccact ggaactccac agtaacccgc
    12181 agaaacagct aaagagtact cttctcattc tccgtacctc attgataatc gacgcgtaaa
    12241 taccccagcg cgctaaacgg cccttcggtc ggtttttgac cggtaatact gataggccag
    12301 gcgcgaagtg tgacattggc tgccgcagct gcatccagac ggaaaggaat aacgctattg
    12361 agatcgttag gcgtgatcgg cgtatcgttc tgatcggcga caataaaacc taaatcctga
    12421 ttgtccgaca ccatcgcctg accagaaacg gcactggctt ccagacgcat tgttaaataa
    12481 gcctgcgcag caacattcgt acatttgacc gcaatgctct tggtttgcgg catgacacca
    12541 gcaggtcgat tacccggccc tgccgcacta aataacgatg cgccgatatc accaaaatca
    12601 aattcaacaa tctgcccggc atttaattcg cagttttgcg gtacttcaac ccggccacca
    12661 aaactaatgg tataaacagg tgtaaccaac ggatcgtaag gcgttgatgt cacatacacg
    12721 gtaaacatca cctgtctggg gatctccacc atgttaataa agggacgaat aacctttaaa
    12781 cgaaatatta aacgagaatc catcaccccg aatggaaggt tttgtgaaac gttaggatct
    12841 gttcccatcc gaatataatt ttgcgggggg aactgaatat ccatcacact atcaaccaga
    12901 ctcatcgcac ctaatagata atcatttaat tgcatatatt tataattatc gatagtttcc
    12961 tgaacaataa agttggtgac ataactacgg tatgtataat tcaccagcgt gccgggtgga
    13021 caaattgctg acacacctac ccagccggat ttttccggta aaacaacaat ctgccctggc
    13081 tggttattgg tgctattaaa gatatttgag agatcatagt gttcatcttc tgctacgcca
    13141 gtactatttg cacaaacagt agcccaggat atgtttgcca gacacaaaca tgccatcgcc
    13201 aataataatc tgcagattat cttcattgat aatttccttt atgagccagg atgtgtgcaa
    13261 actgccgaaa taacagtgac ggcttgctgt aagctttgct tcggcaatac atagtgcgca
    13321 atgcagcgtg agttcgcgcc gtctccccac tgaaccagta attcaccaga caatggcgca
    13381 ccacttaaat aaacttgccc gtcatcaccc accatactgg ttattccggt actgttttcc
    13441 cgtaccagtg atccaaacgg caccggtttt ccgccctggg taacggtaat gagcgcccgc
    13501 acgcctatgc gggtatcaaa attggcacga accaacgcgc cttgcgtcgg cacaacgctg
    13561 ctaatatttt tttcaacatc gatggaattc cccatcgtat tggtatcaag cgcgatacgg
    13621 ttataccgat aaaccgtggc atacggcatc accgcatagc cgcgccagtc ggttaaaatg
    13681 ccagtttgat tttcaatgcg tacaccgcct gcgccaggcg ctttaatcaa aacattggta
    13741 tcccctaaag gctggctcag cgttatgcca ttttcgtgcc cgaccacacc gccagacagc
    13801 tgccagttaa cgtcatgttg atcgcggtcg tagttatacc cgacccccag cgtaccgtaa
    13861 gcggcctgcc agtttgccgt ggcgctgccc gtatacccat tattcgaggt atcaccctgg
    13921 cttacgtgat aactcaggtt gtggccttct agtaaggtcc cacctacacc tgccagccag
    13981 ctattttgcc cgttgctgtt acggttggcg ttaaaggagg cataagcgcg gtcgagcgca
    14041 ttttcccggg tgtagcgacg tttggtcaga acattgaaag gcactgaaac attaagtccg
    14101 acaatacgtt cgttatcggg gatccctaca gattcattcc acgaaaatga gagcgaataa
    14161 ctgatgccaa cccagctgct ggtatacccc acctgatacc aggtatctga atccgaagta
    14221 ttccagtatt tttgatgggt accagagata taaagcgagc caaagtcatt aagtgattgt
    14281 gaaatattta actgcaaacg gtctttacgg ctaaagcgta aattgtggta attcacgatt
    14341 atcagttcat cccgatgctc accgtcgtaa tcgtaatcat attcgtaccc ctccattcga
    14401 cgatacgcaa catcatcaag ggtataaaaa ccttgtgtcg aatagcggta acccattaac
    14461 tgaaaattgg tgccgaaggt gttcatcgat ttcgcataga ggaagcgaat ggaatccccc
    14521 tcatgacgac tgtcgtcggc taactgactg cgcgcatggg tcacatccag cgacactgcc
    14581 ccccagtttc cgagattgcg ccccagtcct aataaaaagg cggtgtaatt ggcagataat
    14641 tgcgtcccgc cgtaggcagt aaattcctgt ggtaaaccgc cgagtgccgt gccctgaaaa
    14701 aagaaaggtg atgattgctg actattaccg ctacgaaaat cgcccgccgt caggtcaaat
    14761 ttgaaacgcc cttcgcgttg taaaatcggc actgttgaat acggaattgt gtaattctgc
    14821 tgattgccat cgcgctcgtc gatggtgaca tccagatcgc catttgaaga tgtcgggtgc
    14881 aaatctgtaa tttcaaaagc gccgggggaa acatagcttt gatagataat aaaaccattt
    14941 tgccgaatcg ttagctgggc cgccgtacgg gcaatcccac gtaccgttgg ggcaaaccct
    15001 tgctggctat caggatacat attatcggaa gagtaaagcc gtataccacg aaatccaaca
    15061 ccatcgaaaa tatcacttcc tgtattgccg tctcccatta ccagttcact tttcagcgga
    15121 ataatggcgc gctgtaccca ggtgccaata ttattccact gttctgaatg atatccattt
    15181 ccgcgaaaat aattccagga accattgttg cgtaaacgcc acgggccaat attaatcccg
    15241 ctgagctcac taaaaaaata gctatcgtta ccattacctc tgttaccggt gaaattataa
    15301 ttcaggagta aagcaggaat accttcatcc cactcttcag gcggaatgta accgtgagcg
    15361 ctactcaaca acgctatctg aggaatagtg atgttcaggc gcatcgctgc aaaatcaaag
    15421 ttaattgtgg catcaggtac agctttcagt aaattgatgc atttattgtt ttgctgctgg
    15481 gctaactcgg gaaatgcact gctattaagg ccaattcgtt caaggagtac ctggttaaaa
    15541 caggacatta acccacctga tttatcacct gtattttctg ttgtcgattc aaatacgata
    15601 tcctgtgaac caatgaactc atcattacgc cagagatcca cccgatacac tcccgcaggt
    15661 tgatgattgc ctctctcaaa gcgcgataaa tcagcaacgg atgtgccatt ctctaataaa
    15721 aatgccgggt tgaaataact ttccgcatag cttaaaaagg agggtaaaat ggcaagagat
    15781 gaataacaaa ttccggccag acaccaggta tacctctgcg gaatatccgt agtagtgggt
    15841 attttcataa ttagctctga tattgtcaga tgattcggtt agtttaagtt cacttctctg
    15901 accggggtta ctgaaccata atcattaacg gtctgaaatt tcagctttcc ctgcactcct
    15961 gagggtaagg gaatttgtga attaactctg ggtgcagcca tagcattccc caacttttgg
    16021 ctgcccactt gtaagttaac cagggtgaca taaaaaggtg agggattatg aacattgata
    16081 tagttaccgg atcgcgaaaa cttgagtgtg tccggcgctt ctgcgggtaa ttcttgtaat
    16141 tgaattggac ggagaaataa tttcatgcgc gataaaatcg ccagttgcaa aacattcctg
    16201 ccgttcaatg catttttatc taccgaaggg atcgtcttaa cattcatcca gaacagagac
    16261 tcacgatctg ccgccagcgg tggaccggtg taaataatac gcaaggtatt ttcgcttttg
    16321 ggttcactaa caaacagtgg cggtgtaatg atgaatgact tttcttttac accgctgctg
    16381 ttttcaatcc acgaattgac cagaaagcgc tcattggtat ggctatttct aatccatacc
    16441 gcagtctgtt gagcatcagc gggataaata atacgcgtgg cacctaatgc gatccctccg
    16501 gcagcatggg tgctggccga aatgattaaa taaaatataa tgagcatcaa taactttatt
    16561 ttagtcatca tagttaatac gccttgtgta ttcctgacgg caaaaataag aaatataaag
    16621 aaatgattgc tttggtttct tacaacacat cattcccttt tcatttaata aagcggcatg
    16681 atacaaagtg aacatgattt cgatttacat tgccaccata ccgcttcaga caaccggtac
    16741 attactttgt tataagacgt gatatataat tcaaaacaac gtggttttga ttattcgtat
    16801 ctcataataa aagtcgcgtc agcatttgct tgccctgctg atgcacttgt accggtgcct
    16861 ttataacgtg cagaaaaatg aagaacgttg gtcccgggga tcaagttcta gttggttgag
    16921 aagctattcc catcaggttt gagaattgcg gatgtattat caagtatttc aataccgaca
    16981 cccgttgctg ttgttgtatt ggtactggat gcaatggcca ataaattatc attgattgca
    17041 tcagcctgac cagaaaatgc aacggcagca ttagcggcaa caacaggatc gcagtcgttc
    17101 aactgaatgg tgaatggaat taatgcagag gtattaccaa cagcattgaa aatatcgata
    17161 cgatattgac cgagtgtgac aacctggtct gccgagtcag tattcactga acaggcagca
    17221 ttcaccagtt cgccttcaaa gtgaattgta ccgccatcta caacggcagc ataactaccc
    17281 gtagcggcga atagtgcggc agccagcgca gacgaaataa atcttaattt catatatatt
    17341 ccttcaat
//
LOCUS       DAZGVQ010000111        17259 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000111 DAZGVQ010000000
VERSION     DAZGVQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.112"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            117..371
                     /gene="bssS"
                     /locus_tag="ACHLAG_003524"
     CDS             117..371
                     /gene="bssS"
                     /locus_tag="ACHLAG_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFP0656684.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            486..1604
                     /gene="solA"
                     /locus_tag="ACHLAG_003525"
     CDS             486..1604
                     /gene="solA"
                     /locus_tag="ACHLAG_003525"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFP0656685.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPDHDNTLFITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1652..1765
                     /locus_tag="ACHLAG_003526"
     CDS             1652..1765
                     /locus_tag="ACHLAG_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFP0656686.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2026..2592
                     /gene="yceJ"
                     /locus_tag="ACHLAG_003527"
     CDS             2026..2592
                     /gene="yceJ"
                     /locus_tag="ACHLAG_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFP0656687.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2596..3171
                     /gene="yceI"
                     /locus_tag="ACHLAG_003528"
     CDS             2596..3171
                     /gene="yceI"
                     /locus_tag="ACHLAG_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFP0656688.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3213..4265)
                     /gene="trhO"
                     /locus_tag="ACHLAG_003529"
     CDS             complement(3213..4265)
                     /gene="trhO"
                     /locus_tag="ACHLAG_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFP0656689.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDVLFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4490..5410
                     /gene="lpxL"
                     /locus_tag="ACHLAG_003530"
     CDS             4490..5410
                     /gene="lpxL"
                     /locus_tag="ACHLAG_003530"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFP0656690.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5582..6808
                     /gene="mdtG"
                     /locus_tag="ACHLAG_003531"
     CDS             5582..6808
                     /gene="mdtG"
                     /locus_tag="ACHLAG_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFP0656691.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            6891..7265
                     /gene="msyB"
                     /locus_tag="ACHLAG_003532"
     CDS             6891..7265
                     /gene="msyB"
                     /locus_tag="ACHLAG_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP0656692.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(7266..7493)
                     /gene="yceK"
                     /locus_tag="ACHLAG_003533"
     CDS             complement(7266..7493)
                     /gene="yceK"
                     /locus_tag="ACHLAG_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP0656693.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(7666..10209)
                     /gene="mdoH"
                     /locus_tag="ACHLAG_003534"
     CDS             complement(7666..10209)
                     /gene="mdoH"
                     /locus_tag="ACHLAG_003534"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFP0656694.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(10202..11737)
                     /gene="mdoG"
                     /locus_tag="ACHLAG_003535"
     CDS             complement(10202..11737)
                     /gene="mdoG"
                     /locus_tag="ACHLAG_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFP0656695.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            12131..13288
                     /gene="mdoC"
                     /locus_tag="ACHLAG_003536"
     CDS             12131..13288
                     /gene="mdoC"
                     /locus_tag="ACHLAG_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFP0656696.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(13296..14777)
                     /gene="clsC"
                     /locus_tag="ACHLAG_003537"
     CDS             complement(13296..14777)
                     /gene="clsC"
                     /locus_tag="ACHLAG_003537"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFP0656697.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRMFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(14719..15252)
                     /gene="ymdB"
                     /locus_tag="ACHLAG_003538"
     CDS             complement(14719..15252)
                     /gene="ymdB"
                     /locus_tag="ACHLAG_003538"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFP0656698.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(15347..15658)
                     /gene="ymdA"
                     /locus_tag="ACHLAG_003539"
     CDS             complement(15347..15658)
                     /gene="ymdA"
                     /locus_tag="ACHLAG_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFP0656699.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(15779..16111)
                     /gene="csgC"
                     /locus_tag="ACHLAG_003540"
     CDS             complement(15779..16111)
                     /gene="csgC"
                     /locus_tag="ACHLAG_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFP0656700.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(16171..16623)
                     /gene="csgA"
                     /locus_tag="ACHLAG_003541"
     CDS             complement(16171..16623)
                     /gene="csgA"
                     /locus_tag="ACHLAG_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFP0656701.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     TIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(16664..17119)
                     /gene="csgB"
                     /locus_tag="ACHLAG_003542"
     CDS             complement(16664..17119)
                     /gene="csgB"
                     /locus_tag="ACHLAG_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFP0656702.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKFLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         4321 a         4411 c         4098 g         4429 t
ORIGIN      
        1 ccccccttta aaaattcggt gaataccctt acttattggt atactgaagt tgcttcagat
       61 aaaaacgcat tgaacctcga ataacgttgt ctagtaacac gaattagggg gccatgatgg
      121 aaaaaaataa tgaagtcatt cagactcatc cgctcgtagg gtgggacatc agcaccgttg
      181 atagctatga tgcgctgatg ttgcgtttgc actaccagac cccaaataag tccgagcagg
      241 aagggactga agttggtcag acgctctggt taaccactga tgttgccaga caatttattt
      301 cgatattaga agcaggaatc gccaaaattg aatccggtga tttcccggta aacgagtatc
      361 ggcgtcatta acatgacatg accatccacc caaaaggcac ctcaccggtg cctttaccat
      421 ttctgggttg tatatctgca ttcagttaat tactctgctt aaaccatgag agcagagaga
      481 aaaaaatgaa atacgatctc atcattattg gcagcggttc cgtaggcgct gccgccgggt
      541 attatgcaac ccgcgccggt ttaaacgtgc taatgaccga cgcccatatg ccaccgcatc
      601 aacacggcag ccaccacggc gatacgcgat taattcgcca tgcttatggt gaaggcgaaa
      661 agtatgtccc actggtactc cgcgcgcaaa cactgtggga tgagctctcc cgccacaacg
      721 aagatgatcc catttttgta cgctctggtg tcattaacct cggcccggct gactccgcat
      781 ttctcgccaa cgtcgcccac agcgccgaac agtggcaact caacgtcgaa aagctcgacg
      841 cgcaagggat tatggcccgc tggccagaaa tacgcgtccc ggacaactac atcggcttat
      901 ttgagactga ttccggtttt ttgcgcagcg aactggcgat taaaacctgg atccaactgg
      961 cgaaggaagc gggctgtgcg cagctgttca actgcccggt caccgcaatt cgtcatgacg
     1021 atgatggcgt aactattgaa acggctgacg gagagtatca ggcgaaaaaa gcgattgtct
     1081 gcgcgggaac atgggtaaaa gacctgctcc cggagctgcc tgtccagccc gtacgcaaag
     1141 tatttgcctg gtatcaggcc gatggtcgct atagcgtgaa gaataaattc ccggcgttta
     1201 ccggtgaact gcccaatggc gatcaatatt atggttttcc ggcagaaaac gacgcgttga
     1261 agattggcaa acataacgga ggccaggtta tccattcagc ggatgaacgt gttccgtttg
     1321 cggaagtggt cagcgatggt tcggaagcct tcccgttctt gcgcaatgta ttgccgggta
     1381 tcggttgctg cctgtacggc gctgcctgca cctatgataa ttcgcctgac gaagatttta
     1441 ttatcgatac cctacccgac cacgataata cactgttcat taccggcctg agtgggcacg
     1501 gttttaaatt tgcgtcagtt ttaggggaaa tagctgccga ttttgcgcaa gacaaaaaaa
     1561 gcgattttga tttaacgcca ttcaggcttt cccgcttcca ataatcatca ttacggcctc
     1621 cagaatgagg ccgtttttac aggagtattt aatgcgtcgg ctgttgcact atctcatcaa
     1681 taatattcgc gagcatctga tgctttatct ttttctctgg ggattgttgg caattatgga
     1741 tctgatctat gtgttttatt tttagttttc cgcactgaca taaactgaca aatatttaat
     1801 ctccctttca tttagcgtga aaaattgaat gagattattc ctgcttctac agcactcacc
     1861 agacatctct gatgctgaac ccactaaact tatgaagatt ctttcattat taattcaaaa
     1921 ttattgaaat aaaaataaat atcattcaat ttgttatttt tgtgttgcaa ttcattctcc
     1981 gacacgccat tttctttctc tcgcaaattc gttttggatg cacttatgtc attcacaaat
     2041 acccctgaac gctatggtgt tatatctgcg gccttccatt ggctaagcgc aattattgtc
     2101 tacggcatgt ttgctttagg tctgtggatg gtcacgctca gttattacga tggctggtat
     2161 cacaaagcac ccgaactgca taaaagcatc ggtattttgt taatgatggg gctggttatt
     2221 cgcgttctgt ggcgtgtcat atctccaccg ccgggtccgc tgccgagtta ttcgcccatg
     2281 actcgtcttg ccgcaaaagc tggacatctc gcattgtacc tattgctgtt cgccatcggc
     2341 atcagcggct atctgatctc aactgccgat ggtaaaccga tcagcgtttt tggctggttt
     2401 gacgtccccg cgacccttgc cgacgctggc gcacaggcag actttgccgg tgccctgcat
     2461 ttttggctcg cgtggagcgt cgtagtactg tccgttatgc acggatttat ggccctgaag
     2521 catcatttca tcgataaaga cgacactctg aagcgcatgc tgggaaagtc gtcatctgac
     2581 tatggagtat aaaaaatgaa aaaaagcctg cttggtttaa ccttcgcgtc cctgatgttc
     2641 tctgccggtt cagcggttgc cgccgattac aaaattgaca aagaaggtca gcacgccttt
     2701 gttaatttcc gcatccagca ccttggctat agctggttat acggcacctt taaagatttc
     2761 gacggtactt ttacctttga cgaaaaaaat ccggctgccg ataaagtgaa tgtgacaatt
     2821 aacaccacca gcgtcgatac taatcacgcc gaacgcgata aacatcttcg cagtgcagat
     2881 ttcctcaata ccgcaaaata tccacaggca acattcacct ccaccagcgt gaagaaagac
     2941 ggtgacgaac tggatattac cggcgatctg acgctgaatg gcgtaaccaa acccgtcacg
     3001 ctggaagcga aattaattgg tcagggcgac gacccatggg gtggtaaacg tgcaggcttc
     3061 gaggccgaag gcaaaattaa gctcaaggac ttcaatatca agacagattt aggtccagct
     3121 tctcaggaag tggatctgat tatttcagtg gaaggcgtac agcagaagta agcgtgaaat
     3181 gttgccggat ggcacgcatc cggcaatgat atttattctg ttggatcagg aatgcccagt
     3241 gttgtattca gacgtccacg agacttatta aagatcttat tgccattttc acgtcctgcc
     3301 cgacggcgtc gctgttcttc tggcggtaac gcgctttctt cgcagcaaat ctcactacaa
     3361 caacctttgt atttttccgc gcatactgga cactgaataa acagcaggtg gcagccatca
     3421 tttttacagt tggtatggct gtcgcacggc gcgccgcact ggtggcaatg cgcgataatc
     3481 tcatccgata tacgttcgcc catccgctcg tcaaaaacaa aatttttgcc aataaaacgc
     3541 accggcaagc cctgctcgcg cgccttacgg gcgtattcaa taattccgcc ctcgatatgc
     3601 cagactttgt tgaatccgtt atgtttcatc caggcactgg ccttttcaca acgaatgccg
     3661 ccggtgcagt acatgacgat ttttttatct ttatgtgcct gcatcatctc gactgctttt
     3721 ggcagctgct cacggaaggt atctgccgga atttcgagcg cgttttcaaa gtgccccact
     3781 tcatactcat agtggttacg catgtcgata aatagtacat cgggatcgtc aagcatggcg
     3841 ttcacttccg ccgcttgcag atactcacca acattgctgg catcaaagtg aggatcgtca
     3901 ataccgtcgg caacaatgcg atcgcgtacc ttcatgcgca gtacccagaa ggatttcccg
     3961 tcatcatcca acgcgatatt caggcgtaaa ccctctaaag ccgggtcgaa ggcatagagc
     4021 tgcgcgcgaa atgtttcaac attgctcgca ggtacgctga tttgcgcgtt aatgccctca
     4081 tgcgccagat acactcgccc aaaaacattc agcgcggtaa acagctgata taaagcgtca
     4141 cgggtcgcct taggatcggc gatgtggaaa tacttgtaaa acgaaatggt ggttcgcggt
     4201 tcgctctcag ccaacatttt ggcttttagc gcgtcgttgg aaatgcggtt gtgtaacact
     4261 ggcatggtgt acggttcctg cgagatggga aagtaaaaat ccgcggcatg atatagcaat
     4321 tatcgataat taacatccac acattttacg ctacatttgc gcattaaaaa ttatttgtta
     4381 tttacaagcg cggcaatttc gcccagtctt cagccacaaa ttttggttgc gggcgaaaaa
     4441 atgcgacaat acatacaatt gcccgaatag gttgaaaaac aggattgata tgacgaatct
     4501 acccaagttc tccaccgcac tgcttcatcc gcgttattgg ttaacctggt tgggtattgg
     4561 cgtactttgg ttagtcgtgc aattgcccta cccggttatc taccgcctcg gttgtggatt
     4621 aggaaaactg gcgttacgtt ttatgaaacg acgcgcaaaa attgtgcatc gcaacctgga
     4681 actgtgcttc ccggaaatga gcgaacaaga acgccgtaaa atggtggtga agaatttcga
     4741 atccgttggc atgggcctga tggaaaccgg catggcgtgg ttctggccgg accgccgaat
     4801 cgcccgctgg acggaagtga tcggcatgga acacattcgt gacgtgcagg cgcaaaaacg
     4861 cggcatcctg ttagttggca tccattttct gacactggaa ctgggcgcgc ggcagtttgg
     4921 tatgcaggaa ccggggattg gcgtttaccg cccgaacgat aatccactga ttgactggct
     4981 acaaacctgg ggccgtttgc gctcaaataa atcgatgctc gaccgcaaag atttaaaagg
     5041 catgattaaa gccctgaaaa aaggcgaagt ggtctggtac gcaccggatc atgattacgg
     5101 cccgcgctca agcgttttcg tcccgttatt tgccgttgag caggctgcga ccacgaccgg
     5161 aacctggatg ctggcacgga tgtccggcgc atgtctggtg cccttcgttc cacgccgtaa
     5221 gccagatggc aaagggtatc aattgattat gctgccgcca gagtgttctc cgccactgga
     5281 tgatgccgaa actaccgccg cgtggatgaa caaagtggtc gaaaaatgca tcatgatggc
     5341 accagagcag tatatgtggt tacaccgtcg ctttaaaaca cgcccggaag gcgttccttc
     5401 acgctattaa atctcccctg ccggatgctt cagaatggca tccggcatta ccacagcaaa
     5461 tccccctgat ttagcgataa aagctctctg gattgcgccc cctggaagtc gggcgcataa
     5521 ttagtgtgct tatcttttct tcttatgttc accgcgcctg gcgcaccaac agcggattgc
     5581 tatgtcaccc tgtgaaaatg acacccctat aaactggaaa cgaaacctga tcgtcgcctg
     5641 gctaggctgt tttcttaccg gggccgcctt cagtctggta atgcccttct tacccctcta
     5701 cgttgagcag cttggcgtta ccggtcactc cgccctgaat atgtggtccg gtattgtctt
     5761 cagcattaca tttttatttt cggccatcgc ctcaccgttt tggggtggac tcgccgaccg
     5821 taaaggccga aaactcatgc tattacgctc tgccctcggc atgggcatcg tgatggtgtt
     5881 gatgggactg gcacaaaata tctggcagtt tttgatccta cgggcgcttc ttgggttact
     5941 tggcggattt gtccccaacg ctaatgctct tatcgccaca caagtaccgc gtaataaaag
     6001 cggctgggcg ctgggtacgc tctccacagg cggcgttagt ggtgcgttgc tcggcccaat
     6061 ggctggcggc ctgctcgccg atagctacgg cttacgtccg gtattcttta ttaccgccag
     6121 tgtgctcata ctctgctttt tcgtcaccct gttctgcatc agagaaaaat tccagccggt
     6181 cagcaaaaaa gagatgctgc acatgcggga agtggtgaca tcacttaaaa acccgaaact
     6241 ggtactcagc ctgtttgtca ccacgttaat catccaggtg gcgacaggct caattgcccc
     6301 cattctgacg ctgtatgtcc gcgaactggc gggtaacgtc agtaacgtcg cctttatcag
     6361 tggcatgatc gcctcggtgc caggcgtggc ggctctgcta agcgcaccac gactcggcaa
     6421 acttggcgat cgaatcggac ccgaaaagat cctgattaca gcgctgatct tttctgtact
     6481 gctgttgatc ccaatgtctt acgttcagac gccattgcaa cttgggattt tacgtttttt
     6541 gctcggtgcc gccgatggtg cactactccc cgccgtacag acactgttgg tttacaactc
     6601 gagcaaccag atcgccgggc gtatcttcag ctataaccaa tcgtttcgtg atattggcaa
     6661 cgttaccgga ccattgatgg gagcagcgat ttcagcgaac tacggtttca gagcggtatt
     6721 tctcgtcacc gctggcgtag tgttattcaa cgcagtctat tcatggaaca gtctacgtcg
     6781 tcgtcgaata ccccaggtat cgaactgatt tttcgccttt catacttgca aaagcggaga
     6841 atcagctatc cttttccctg aaacctcatc aactcaaagg gagaatcgtg atgaccatgt
     6901 acgcaacgct tgaagaagcc attgacgctg cacgcgaaga atttcttgca gacaaccccg
     6961 gcatcgacgc cgaagatgcg aatgtgcaac agttcaatgc ccaaaaatac gttttgcagg
     7021 acggcgacat catgtggcaa gttgagtttt ttgccgacga aggggaagaa ggtgaatgtt
     7081 tacctatgct tagcggtgaa gccgcgcaaa gtgtttttga tggcgactat gatgagatag
     7141 agatacgcca ggagtggcag gaagagaata cattacatga atgggacgag ggggaatttc
     7201 agcttgagcc accgctggat accgaggaag gacgcgcagc agctgatgag tgggatgaac
     7261 gttaatcact catacgggcc atgatgagtg tcgatcggca gcagtaaagt atcaaaaacc
     7321 agagagaatg gcagatcaag gatcgtgaca taacgccagg cggagtcacg cacatcccat
     7381 tgcacaccgg gataatattg gttgccatgc ccctgccccg gtatagtgcg actaataatg
     7441 ctgccacagc cactcagcaa ggtcaccatg atgctcacca caattaaacg catttgatac
     7501 ctctttcgta aaaaaatacc ggcataacgc cggtattttt attggctcgt tgtttttgcc
     7561 ggatgcggcg tgaacgcctt atccgaccca caaaatcttg caaaatcaat aaattgcagg
     7621 aacgatgtag gcctgataag cgtagcgcat caggcaacta cgtttttatt gcgaagccgc
     7681 atccggttta cgcaatgcca gtggattgag ctttatccct tcgtaataac tcacccatga
     7741 agaatatctc tccggggaat tccagacgcg gaaatgcaga cgggccatcg tcaccggatc
     7801 gcttagcagc accaggcgac gatcgcgatt cagcttctct ggcgtctcgt tcagcgcctg
     7861 ttcaacgtgg cggtcacggg cgatttccag caccttactg gcgcggtgac gcgcggtcgc
     7921 cattgcggtt gccagagcgt taaatgacgg gttaaacact gcgtgcataa agccatcatc
     7981 aagggagcgt tgacgattca tctcaaggaa ccgatcggta tcaaccagca cctgcggcgg
     8041 cgaatactct tccgggatca gaaacagttt ccagcgtttg gtgcgcagac caacggtggc
     8101 acggctggaa atcaccgaaa caaacggtga caggatcaac gagaagacaa tcggtgccag
     8161 ccagaacagg aaacgcagat ccagccacgc catcccaaca gcccacacta accctaacag
     8221 cagctgtgag ccgtggcgtt tgaacgcttc accccaggaa gtggagtcat catcacgctg
     8281 cggtgaattc cacaccactt cccagccaag gaacgcgctg acaacgaaga ccgtatggaa
     8341 cagcatgcgt accggagcca gcagcacgga aaaaagcact tccagcagca acgataatgt
     8401 aacgcgccag aagccgccgt attctttcgt tcctttgcac cagataagca aaatgctcaa
     8461 taacttcggc aggaacaaca gcaccatagt cgaagcaaaa agtgcaatcg ccagctcagg
     8521 acgccactgc ggccacaccg ggaacaactg ccgtggttgc aggaagtatt gcggttcggt
     8581 caacgcatgt actacctgca atgcagtaga gagcgcgagg aacataaacc acagcggagc
     8641 ggagagataa gacatcacgc ccgtcaggaa caccgcacgg tgaaccgggt gcataccctt
     8701 caccaggaac agacggaagt tcatcaggtt accgtggcac cagcggcggt cacgttttag
     8761 ctcatcaagc aagttaggcg gtaattcttc ataagaaccc gggagatcgt aagcaatcca
     8821 gaccccccaa cctgcacggc gcatcaacgc cgcttctaca aagtcatgtg acaggattga
     8881 accggcaaag gaaccttcgc ccggcagcgg agccagtgcg cagtgctcga taaacggttt
     8941 cacgcggata atcgcgttat gtccccaata gtgcgactcg ccaagttgcc agaagtgcaa
     9001 accggctgta aacagtggcc catacacgcg ggtcgcgaac tgctgacagc gcgcatacag
     9061 cgtatccata ccggacgctt tcggcgacga ctgaatgatc ccggcgttcg ggttggcttc
     9121 catcaggcgc accagcccgc acaaacaatc accggtcatt accgagtcag catccagcac
     9181 caccatgtag ctgtactggc tgccccagcg acggcagaag tcatcgatat taccgctttt
     9241 acgcttcacg cggcgacggc ggcggcgata gaaaatctga ccttcgccac cgacttcagc
     9301 gataagctcc atccaggctt tttgctctgc gacgcagata tccgggttat aactgtcact
     9361 aagaatgtag acatcaaagt gtttggcatt cccggtggct tttactgatt cccacgttgc
     9421 acgcaggcca gcaaaaacac ggttcacgtc ttcgttacag ataggcatga tcaacgccgt
     9481 gcgatgctcc gggtttaatg gttcatcgcc aactgttgac gcagatatac tgtatttatc
     9541 gcgaccaata agcagttgca ggaagcccat taacgccgtc cagaatccgg cggacaccca
     9601 acagaacagt accgcaaaga ggatcaggat accggtttgc agcatataag gcagaagctg
     9661 cataaaggaa acccacaaat cctgaccaac catatccata ggattaatca gcgcccaacc
     9721 ctgataagga agaatggtct tcatatacca ggtcgcgacg acagtttgcg cgagcgtcag
     9781 gatcaacaga atgtaacggc ggatggtacc gacggtacgc cacttttgct cgctctcctg
     9841 ctcttctttg gtcaaacgag ccagatagcg cggcgtgaca tcgcgtccac gcaggcgatc
     9901 ccagaaacgg cctaccgggt tggtacgcca cgggtcggga aacatcgagg agcgttttgc
     9961 ttctggcatc gccttcagct gatcgcgccc ttcgtcgtct ttaattaact gtccatcagc
    10021 aagtgaatct ggccaggctt gttccagacg cgcctttacc gagccttgcg gggaatcatc
    10081 ctcccgcgcc caggtgcggt gttcggcatc cagcgcctga tgaacggcgc ggatatcagt
    10141 cttcggcaat gccgctttct cgcttgcggc gatgggcatt gcgtcaatgt actcagttgt
    10201 cttattcatt ggcaggtaac tggtagctcc aggtttcact caacgtctga tcggcattca
    10261 ccagcgcagc acgcatttca gtggttttct tggcatcttt cactttcaca cgcatcacca
    10321 gacgccagcc tttggtaacc gggttatagc gcaccgtgct ttcaactatc tcaccattat
    10381 caccaatgct ggtttgcgct gtgaccgggg tatcctctgg cagttttttc atctctgcgc
    10441 cggtaaaatc gaccacaaag gcgatagtac cgtcaggctg gcgaatcagg ttcgactgct
    10501 tcacatcccc cgttgaacga cgcgtttgtt gcacccatgc gttatctggc gcatgcagtt
    10561 tgtcttcatc acggctgaag gtgatggtgt atttaaagtt catctcttta cccggctccg
    10621 gcagctgatc cggcgtccag taagcgacga tgttatcgtt ggtttcatcg ttggttggaa
    10681 tttccaccag ctcaacgctg cctttacccc actccccttt cggagtcacc catgcgcttg
    10741 gacgaagatc gtaacgatca tcgagatctt caaagcggga gaaatcacga ccgcgctgca
    10801 acagaccgaa gccttgcggg ttctccatcg agaagctgct gaccgctaaa tgtttcgggt
    10861 tattcaacgg acgccagatc cattcgccat taccggcatg gatagagaga ccgttagagt
    10921 cgtgcaactc cggacgatag ttatttgcag gcgacggttg gttcggccca aacaggaaca
    10981 tactggttaa cggtgcaacc cccagtttgc cgactttatc gcgcagatag attttcgact
    11041 gcacatccac aaccgtgtca cgccctggca taactacgaa tttgtaagca cctgtcgcgc
    11101 gcggcgagtc aagcaatgca taaatggtta aacgtttatc agtcggtttt ggacgctcga
    11161 tccagaactc tttgaagcgt ggaaattctt cacccgatgg caaggcggta tcaattgcca
    11221 ggccgcgggc agaaaggcca taaacctgac ctgcaccaat cacgcggaaa tagctggccc
    11281 cgagcatgct gacgatttca tcgtttttat ctttgctgtt gatcgggtaa agcactttaa
    11341 aaccggcaaa accaaggtct tttaccgtgt ctttgtcatg ctgaacatcg ccgaaagtga
    11401 aataatccgg gctgtatttg attcgtttga ctgcggtggc agtcacttca tttattttga
    11461 ccggggtatc gaagtacata ccctgatggt agaactcgag tttgaatggg gtcttcagat
    11521 tgttccagta cgctttgtca tgattaaact ggatctgctg atagtccgcg tatttcatat
    11581 cgcggaaaac ggagggcaag ttgcttttgg gcgcctcata gcctttcccg gctaaggatt
    11641 gagcttgctt tgcgacatca tcaatactga aagcccagct tgaagatgta tacagggtta
    11701 acattactgc agcactcaac caacgcattt tcatcatttg tagtttatgt ttcataatta
    11761 gtaagcactt ccccctttgt gtgcttatat cgatccgatc tattttaatg gataaccagc
    11821 cattccgaca acagaatccc ggtatcgttc agcacgctgg ctcatggatt aagcacctga
    11881 ttgcaaccgt tttcactttc cgtgcttctc ctaaagacag aagcatcgac ttcgtgtagg
    11941 gtttgccagc aattttattt atcaaccgtc ttacagacgc ttccattctg gttagtggta
    12001 gcgatgatgc tatgcataaa aaccttgaag cctgcgcctg gcgcggtcag gtcggtgtcg
    12061 ctatggcaac aagcctaaag gcataacccg acagggataa gacgaaaaat ccgagattag
    12121 ttaaccatat atgaatccag tacccgcgca acgtgaatat ttcctcgact ccatccgcgc
    12181 ctggctgatg ttgttaggga tcccttttca tatttcttta atctattcga gccatacatg
    12241 gcatgtgaat agcgccgaac catcattgtg gctgaccctt tttaatgact tcatccactc
    12301 gttccgcatg caggtatttt tcgttatatc cggctacttt tcctacatgc tttttttacg
    12361 ctatcccttg aaaaaatggt ggaaagtacg tgtcgaacgt gtaggtatcc cgatgttaac
    12421 agccatcccc ctactgacat taccgcaatt tattatgctg caatatgtca aaggaaaagc
    12481 ggaaagttgg cctgggctgt cattgtatga caaatataat acgttggcct gggaattaat
    12541 atcacacctg tggtttttac tggtgttagt agtcatgacg acgctgtgcg tatggatatt
    12601 taagcgcatc agaaataatt tagaaaattc tgataaaacg aataaaaaat tctcgatggt
    12661 aaaactatcg gtgatttttt tatgcctcgg catcggttat gcggtaataa gaagaacgat
    12721 ttttattgtg tatccgccca ttctgagtaa tggcatgttc aattttattg tcatgcaaac
    12781 gctgttttat ttgccgttct ttatcctcgg cgcactggct ttcattttcc ctcatcttaa
    12841 agccttgttt accacgccgt ctcgtggctg tacccttgca gcagcattgg cgtttgtcgc
    12901 ttatttactc aaccagcgct atggcagtgg cgatgcctgg atgtacgaaa ccgagtcggt
    12961 gatcaccatg gtcctcggtc tgtggatggt gaatgtggtc ttctcctttg gccaccgttt
    13021 gcttaacttc cagtcagcgc gggtgactta ttttgttaac gcatcgctgt ttatctatct
    13081 ggttcaccac ccgttaacgc tgtttttcgg cgcatacatt acaccgcaca tcacctccaa
    13141 ctggcttggt tttctctgtg gcctgatatt tgtagtaggg attgcgataa ttctgtatga
    13201 aattcatttg cgcatcccgt tactgaagtt tttgttctct ggtaaaccgg ttgttaagcg
    13261 tgagaacgat aaagcaccag cccgttaagc cacatttaca ataaccattc cacgggcaat
    13321 atcgacgcca gtctgaccat aacccgcttc cagaaactgg tggcgggttc ttttttgaga
    13381 ataatctctt tctttgcatg acgatcaacc cagttgatcc gtccccacct gtccagacgg
    13441 agctgccagg ccgcatcata ctggctctga ataaagcgtt tatcaattaa ctgtgccagc
    13501 gtttcgctct ctatcacgaa gcccatttca gtattgagca atgttgaacg cggatcgaaa
    13561 ttgaaagagc cgataaacac cgttttacca tcgatgctaa aggttttagc atgcaggctg
    13621 gctccggaat taccggttat gccgcgatcg tgtaacgtac tactttgttc acgcgtcggc
    13681 ttgagttcat ataattccac gccatagcgg agcaattttt tgcgccagcg cgcgtatccg
    13741 gcatggacga cagcaacatc gttagcggca agagaattgg ttaggatcgc aatctttacc
    13801 ccttttctca ccatccgtaa gagttgcgcc acacctgcgc gtgtcggtac aaaataggaa
    13861 gagataatat cgatgcgttc actgggtgag cccatgatat cgaacatgcg ctgcggtaac
    13921 agtgaatgcc gttttgcctt gccctcccct ttcgccggat catcacttaa taaacgtgtt
    13981 ttcgcccaga taagcggcaa cgttccatca accagatgat ttataaacgg actggattcc
    14041 attttgcgta agtaacgatg cgtcatagca tcgttatgcc aggaggcggg taactcgatg
    14101 cgatccgcca tttcaccttc cgggacatcc agcacttgct gtaagggtga aaccgatttg
    14161 caataccagt agcgggcgaa atcatcggca acgtcctcta ccacgggccc tattgccata
    14221 acatctaaat ccgaaaaaag tggctcttcc cctgccccaa aataggcatc accaatattt
    14281 cgccccccta ccagcgtcac cacgccatcg acagtgaaac ttttattgtg catacggcga
    14341 ttgagacggg aaaagtcggt gatataacca agcggacgca gcaagcgaaa cgagaaagga
    14401 ttaaaaagcc ggacttcaat gcggggatgg ctgtcaagca agcgtaaaat gtcgtcaagt
    14461 ccgggcgtat tgttgtcgtc cagcaacaaa cggacgcgaa cgccacgctt tgctgcggct
    14521 aacagggcgg aaaacagtaa ccgacccgac atatcatcct gccagatgta atactgaaca
    14581 tccagcgtat gctctgccat ttcggccaga cgataccggg cggcaaacgc atccagactt
    14641 ttctccagag ggaaaagacc acactgaccg ggatgttgcg aacacagtgg cagcaccgcg
    14701 ctcgccagcc ggggcaaatc attcatctcc ttgttgggta aggagtcttt cgtagaggtg
    14761 ggcgttttct tcatcataac agacaaagta tacctgttcg ggtaaagcgt gacgggtaat
    14821 aaattctgaa acggttttaa ctgcgatttc agccgctgcc gcacgagggt aaccataaac
    14881 cccagtactg attgcaggaa aagccactga cgtatagctg tttgccgcca ccagtcgcag
    14941 gctattcaga taggcatcct gcaaaagctg gtcttcgttt tgttcaccac cacgccagac
    15001 tggcccgacg gtgtgtacta cggctttagc gggaagatcg cctgcaagcg taataacggc
    15061 atgccccgta gggcaatcgc cctgctgttg cctgactttt aaacaggcat ccagcagggc
    15121 cggacccgct gcgcgatgaa tggccccatc gacgccgccg cctcccatta atgacggatt
    15181 agccgcattc acaatcacat caacggccag tttggtaata tcaccctgca caacatgaat
    15241 acgcgttttc ataacgcctc cttacacctt cttgtttctt aagtgtacag caggcttttt
    15301 ggttaaatcg catttttctg ctcagcaacg tgcgttgttc agaaacttag ttatattcga
    15361 tgttcattac agccagcttt ttctgtttat caagatactg cacttttact gaggctattt
    15421 gcgcgttttt aacattaccg ctcattaacg ctttgctgga ataggttttg cccggaacag
    15481 aaccattctg tgggcagttc atttctacgg gtgatgactg ggtgctgacg tcacatgccg
    15541 gttccacaat ttggccataa aaatggatca cccccccttg cgcggtgctt cctgcgatgg
    15601 caataaaagg aaaaaacaaa ctgccgagca taagagagtt taaaaaaggt ctgaacattt
    15661 attctatttc ctcaatgatt agtcatcctt gagggttgtg ttatccatac tttcaggttg
    15721 gctgattcgc agcagaccat tctctccaga ttcatcttat gctcgatatt tcaacaaatt
    15781 aagatttttc tgaagagggc ggccattgtt gtgataaatg aagtgactgt ccatcagaaa
    15841 cagtaacaac tattttcacc cgatcgtccg gggaaatatt taaactcaac ttcgtcaaag
    15901 caatgggttg attagcaggc aatgaaaggg tcttttcttg cttcgtctga ctttgccctg
    15961 aactgccttc gcgcagggac aatatttgta ctctgcacag acaagattga gtaagagtga
    16021 cttcaggaat aatggtatac acatcccctt gctgggtcgt attaaaggtt atctggctgg
    16081 aaagtgccgc aaggagtaat aacgcattca tattcttctc ccgaaaaaaa aacagggctt
    16141 gcgccctgtt tctgtaatac aaatgatgta ttagtactga tgagcggtcg cgttgttacc
    16201 aaagccaacc tgagtcacgt tgacggagga gttagatgca gtctggtcaa ctgcagcacc
    16261 gttgccacca ccgaactgtt taaccgtcat ttcagaattt ttgccgttcc actgatcaag
    16321 agtagcgctg ttaccgaagc cacgttgggt cagatcgatt gagctgtcat ccgagccctg
    16381 accaacatct gcaccattac cgccgccatg ctgggtaata gtcaagtcag agttacgggc
    16441 atcagtttgc agagcaagtg cagagttacc gccaccgtac tggtaaatgg tcagctctga
    16501 atttgggccg ctattattac cgccaccacc gtggttaccg ccgccgtact gaggaacaac
    16561 acctgccaga gcgctaccgg agaatacgat tgctgcaatt gctgctactt ttaaaagttt
    16621 catgtaaaac ccccatcgga ttgatttaaa agtcgaatgg aaattaacgt tgtgtcacgc
    16681 gaatagccat ttgcgactgt ctctgcacta caattgccgt tttttgagta ccatactgtg
    16741 taatatttgc tttattacca gaacctttct ggataatcat cgcagtatta ccataagcac
    16801 cttgcgaaat actggcatcg ttggcactgc ccgcctgatc aatatatgca aggttataat
    16861 ctcctgtctg gtcaatcttt gcccgattgc tactaccttc ttgcgcaaca accgccaaaa
    16921 attttgagcc tccctgccgt aactgagcac tattattcgt cccagcttga ccaattatgg
    16981 ctgcctgatt aaatgaagac ttactcaatt catttaccgc gaagttatat tctgaattag
    17041 ctaaatcata acctgctgcg gctgcaatcc caggcgcacc cagtattgtt aacatcataa
    17101 ataacaattt gtttttcatg ttgtcaccct ggacctggtc gtacatttaa gaaattaaat
    17161 catttcaact tggttgttaa cgcaacctgt attttgttaa cgctgcgtta cgatggaaag
    17221 tatgtctgcg gaaatatttt taataactca cccgcgcgt
//
LOCUS       DAZGVQ010000112        16604 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000112 DAZGVQ010000000
VERSION     DAZGVQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16604
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.113"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            75..1760
                     /locus_tag="ACHLAG_003543"
     CDS             75..1760
                     /locus_tag="ACHLAG_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFP0656703.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(1935..2471)
                     /gene="rcdA"
                     /locus_tag="ACHLAG_003544"
     CDS             complement(1935..2471)
                     /gene="rcdA"
                     /locus_tag="ACHLAG_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFP0656704.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            2555..3763
                     /locus_tag="ACHLAG_003545"
     CDS             2555..3763
                     /locus_tag="ACHLAG_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0656705.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFIDSAWVAGVSVVLWGLGASLGFPLTISATSDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            3763..4578
                     /gene="ybjI"
                     /locus_tag="ACHLAG_003546"
     CDS             3763..4578
                     /gene="ybjI"
                     /locus_tag="ACHLAG_003546"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFP0656706.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            4664..4948
                     /gene="ybjH"
                     /locus_tag="ACHLAG_003547"
     CDS             4664..4948
                     /gene="ybjH"
                     /locus_tag="ACHLAG_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFP0656707.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(4989..6221)
                     /gene="mdfA"
                     /locus_tag="ACHLAG_003548"
     CDS             complement(4989..6221)
                     /gene="mdfA"
                     /locus_tag="ACHLAG_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFP0656708.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            6506..7102
                     /gene="ybjG"
                     /locus_tag="ACHLAG_003549"
     CDS             6506..7102
                     /gene="ybjG"
                     /locus_tag="ACHLAG_003549"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP0656709.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGFTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            7160..7918
                     /gene="deoR"
                     /locus_tag="ACHLAG_003550"
     CDS             7160..7918
                     /gene="deoR"
                     /locus_tag="ACHLAG_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFP0656710.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(7965..9167)
                     /gene="dacC"
                     /locus_tag="ACHLAG_003551"
     CDS             complement(7965..9167)
                     /gene="dacC"
                     /locus_tag="ACHLAG_003551"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP0656711.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            9414..10040
                     /gene="gstB"
                     /locus_tag="ACHLAG_003552"
     CDS             9414..10040
                     /gene="gstB"
                     /locus_tag="ACHLAG_003552"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFP0656712.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(10037..11152)
                     /gene="yliI"
                     /locus_tag="ACHLAG_003553"
     CDS             complement(10037..11152)
                     /gene="yliI"
                     /locus_tag="ACHLAG_003553"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFP0656713.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FVALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(11263..11646)
                     /gene="bssR"
                     /locus_tag="ACHLAG_003554"
     CDS             complement(11263..11646)
                     /gene="bssR"
                     /locus_tag="ACHLAG_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFP0656714.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            11859..13184
                     /gene="rimO"
                     /locus_tag="ACHLAG_003555"
     CDS             11859..13184
                     /gene="rimO"
                     /locus_tag="ACHLAG_003555"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFP0656715.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(13231..14559)
                     /locus_tag="ACHLAG_003556"
     CDS             complement(13231..14559)
                     /locus_tag="ACHLAG_003556"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP0656716.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(14567..>16604)
                     /locus_tag="ACHLAG_003557"
     CDS             complement(14567..>16604)
                     /locus_tag="ACHLAG_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0656717.1"
                     /translation="NKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRP
                     WSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDLLAVDKIHVDLNWRYLNEY
                     LDQISANDEVLFLKQGHEIIAKNQLAREKLIIYNSEGNYNIIDSVDTEYIAKTSAVPN
                     NALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVVYFMTTRVFRRQFSEMTELVNT
                     LAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDAEIERSNKLLSLISYDQESGFI
                     KNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIV
                     TFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAE
                     KLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDIN
                     TRAILGAEALCRWVSAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRA
                     SLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYL
                     NIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFD
                     LARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         4162 a         4161 c         3960 g         4321 t
ORIGIN      
        1 aattctggta aaaaacatta tcgtaaatta ccatttcttt caacagctta ctagtaaaca
       61 agaagttagc ctccgtgaat ataaacgtcg ccgaattgtt aaatgggaat tacattctgt
      121 tattatttgt ggtcctcgcg cttgggctat gtctcggaaa gttacgactt ggttcgatcc
      181 aactgggtaa ttccattggc gttttagtcg tatcgctgtt attaggccaa caacatttca
      241 gcattaacac cgatgcgctt aatcttggct ttatgctgtt tattttctgc gtcggggtcg
      301 aagccggacc gaactttttt tccatttttt ttcgcgatgg gaaaaattac ctaatgttag
      361 cactggtgat ggttggcagt gcgctggtga tcgccttagg gttaggtaag ctgtttggct
      421 gggatattgg cctgacggcc ggtatgttag caggctctat gacgtcgaca ccggttctgg
      481 tcggtgctgg cgatacactg cgtcattccg gcatggaaag caggcagctc tcactggcac
      541 tggataatct gagcctcggg tatgccttaa cctatttaat cggtctggtg agtttgattg
      601 ttggtgcgcg ttacttgccg aaattgcagc atcaggactt acagaccagc gcccagcaaa
      661 tcgcccgcga acgtggcctg gacactgatg ccaaccgtaa ggtttattta ccggtgatcc
      721 gcgcctatcg cgtcggcccg gagctggtgg cctggaccga cggcaaaaat ctgcgtgaac
      781 tgggtattta tcgacaaacc ggctgctaca ttgaacgtat tcgacgtaac gggattctgg
      841 caaatccaga cggtgatgcc gtgctacaaa tgggcgatga aatagcgttg gtaggctatc
      901 ccgacgccca tgcccgactc gatcccagct tccgtaacgg taaagaagtt ttcgatcgtg
      961 accttctcga catgcgtatc gtcactgaag aagtggtcgt taaaaaccat aacgctgtag
     1021 gtaaacgtct cgcacaactg aagttgaccg atcacggttg cttccttaac cgcgtcattc
     1081 gtagccagat tgagatgccg atagatgaca acgtcgtgct taacaaaggt gacgttttac
     1141 aagtcagcgg tgatgcccgt cgcgtaaaaa ccatcgccga tcgcatcggc tttatctcga
     1201 ttcacagcca ggtcactgac ctgctggcat tttgcgcctt ctttgttatt gggctgatga
     1261 tcgggatgat caccttccag ttcagcacat tcagtttcgg catggggaac gctgccgggt
     1321 tgttattcgc cggaattatg ctgggcttta tgcgtgctaa ccacccgacc ttcggttaca
     1381 ttccgcaagg tgcattaagc atggtgaaag agttcggctt gatggtgttt atggcaggcg
     1441 ttggtctgag cgccggtagc ggtattaata acggcctggg cgcgattggc ggtcagatgt
     1501 tgattgccgg attaattgtc agtcttgtgc ccgtggttat ctgtttcttg ttcggtgctt
     1561 atgtattgcg aatgaaccgc gcactgttgt tcggcgcaat gatgggcgca cgcacctgcg
     1621 cgccggcaat ggagatcatc agtgatacag ctcgcagtaa catccctgcg ctgggctatg
     1681 cgggcaccta cgcaatcgcc aacgtcctgc tgacgctggc agggacaatc atcgtcatgg
     1741 tatggccagg attaggataa aactgaagtt gccctgaaaa tgaaattttt ttgcacaacc
     1801 gcagaacttt tccgcagggc atcagtctta attagtgcca ctgcttttct ttgatgtccc
     1861 cattttgtgg agcccatcaa ccccgccatt tcggttcaag gttgatgggt tttttgttat
     1921 ctaaaactta tctactaccc tgcaaccctc tcaaccatcc tcaaaatctc ctcgcgcgat
     1981 agcggcttac ggtcggtgac aaaatgcagc gtcatcccct caataaacgc atcaagcgcg
     2041 cgggcggttc cgggttcaaa ccattgttcg agcgtttgct gactgcgctg catccagttt
     2101 tgcattaccg tttttaatag cggttttcgg ctagccagcg cgtagagctg gtacatcagc
     2161 tccatattat ccggcgttgc aacctgtgag ctgtagatca tatcggtgat agcctggcat
     2221 gcgccttgag catcactaac atcgctaaaa aatgcctgat attgccggga cataatctca
     2281 gtaaaactgc tgaacgcctc cagcaacaac tcatcaattc ctgaaaaata gtaggtcatc
     2341 gatcccaacg gtaccccggc aagggtagca attttgcggt gcgtaacagc atgtattccg
     2401 taaagtttca ccgcctccag cgtggcctgg ataatttttt ctcgccgttg cggatcgtta
     2461 gcgcgacgca tgtcatttcc tctctctgca attgtgtaca aatgtacaca accttgctaa
     2521 tgttgtcttc cctcttctta ttttgacgtg atctatgacc gtaaattctt cacgtaatgc
     2581 attgaaacgc cgaacctggg cgctgtttat gttcttcttt ttgccaggcc tgttaatggc
     2641 gtcctgggca acccgtacgc ctgctatccg cgatattctc tctgtctcga tcgctgaaat
     2701 gggcggtgtt ctctttggtc tgtcgatcgg ttcgatgagc ggtattctct gctcggcgtg
     2761 gttagtgaaa cgctttggca cgcgtaatgt catcctggtc acgatgtcct gcgcattgat
     2821 cgggatgatg atattaagtc tggcactctg gctgacatcg cccctgctct ttgccgttgg
     2881 tctcggcgtc tttggggcaa gttttggttc tgcggaagtg gcgataaacg ttgaaggtgc
     2941 tgccgttgag cgagaaatga ataagacggt tttgccgatg atgcacggtt tttatagcct
     3001 gggcacgctg gcaggcgcag gtgtcgggat ggcactgacg gcctttggcg ttccggcaac
     3061 ggtgcacatt ttattggcgg cgctggtagg tatcgcacct atttatatcg ccattcaggc
     3121 aatccctgac ggtacgggca aaaatgctgc cgatggcacc cagcatggcg aaaaaggcgt
     3181 acctttttat cgcgatatcc agttgctgct gataggtgtt gtggtgctgg cgatggcctt
     3241 tgccgaaggt tctgccaacg actggttacc cttattaatg gttgacggtc acggttttag
     3301 ccctacttcc ggctcgctga tttatgccgg ttttaccctg gggatgaccg ttggacgctt
     3361 caccggcggt tggttcatcg accgttacag tcgcgttgcc gtggttcggg ccagtgcact
     3421 aatgggggcg ttgggtattg ggctgattat ttttatcgat agcgcctggg tcgctggggt
     3481 gtctgttgta ctctggggac tgggtgcctc gctgggcttc ccgctgacca tttctgccac
     3541 cagcgatacc ggccccgatg caccaacccg cgtcagtgtg gtagcaacga ccggttatct
     3601 ggctttcctc gtcgggccgc cgctgctggg ctatctcggc gaacattatg gattacgtag
     3661 tgcaatgctg gttgtactgg cgctggttat tctcgcggct attgtcgcga aagccgtcgc
     3721 caaacccgat accaaaacgc agacggcgat ggagaatagt tgatgagcat taaattaatt
     3781 gcggtagaca tggatggtac tttcttaagc gatcaaaaaa cctataaccg tgagcggttt
     3841 atggctcagt atcagcaaat gaaagcacaa ggcattcgct ttgtggtcgc cagcgggaat
     3901 caatattatc agttgatctc tttcttccct gaaattgcga atgaaattgc ctttgtggct
     3961 gaaaacggcg gctgggtagt gagtgaaggc aaagatgttt ttaatggcga gctgtcgaag
     4021 gatgccttta ctactgtcgt ggaacatttg ctgacgcgcc cggaagtgga aattattgcc
     4081 tgcggaaaaa atagcgccta tacgctcaaa aaatatgacg atgccatgaa aacggtggcg
     4141 gaaatgtatt atcaccgtct ggaatacgtc gataactttg acaacttaga agatattttc
     4201 tttaagtttg gcctgaatct ttccgatgaa ctgattccac aagtacaaaa agcattacat
     4261 gaggccatcg gcgatattat ggtgccagtc cacaccggca acggcagcat cgatctgatt
     4321 atccccggcg tacataaagc caatggcctt cgccaactgc agaaattatg gggaatagac
     4381 gacagcgaag tggtggtctt tggcgatggc ggtaacgata ttgagatgct gcgtcaggca
     4441 ggctttagtt ttgcaatgga aaatgccggc agcgcggtcg tcgcagcggc aaaataccgg
     4501 gcaggctcca ataaccgtga aggcgtactg gatgtgatcg ataaagtgct taaacacgaa
     4561 gcgccatttg accaataaaa acggcagcac gcccggcaac acaatacctg aatgaaggct
     4621 gcataattcg cggaaattaa cccgctgcat tatttcaggc gaaatgatta tgaaaaattg
     4681 tctactgttg ggcgcgcttt taatgggctt tactggtgtg gcgatggcgc aaagtgtcac
     4741 cgtcgatgtg ccgagtggat acaaagtggt tgtggttcct gattcagtca gcgttccaca
     4801 agcggtcagc gtcgcgactg tgccgcaaac cgtctatgtg gctcctgcgc cagctccggc
     4861 ttaccgcccg catccttatg ttcgccatct tgccagcgta ggtgaaggga tggttatcga
     4921 acaccagatt gacgaccatc accattaatt cgagaatgcc tgatcgcaca aagcaatcag
     4981 gcattttttt acccttcgtg agaatttccc atctgtttat cttttaaaaa gataaccatc
     5041 agcgacagcc acaaaattcc gttgacaagg ttgaagagat taaactgtcc gttgcccccg
     5101 ttcagccagg catgtttgct gatttcaata ccaacggtaa agatcagcat ttgcagcatt
     5161 cccatcgccg cagaaaccgt acctttactc atatcgctgg caaacagggt taatcgcacc
     5221 agtcccgcat tcgccagacc aataccgaaa gcataaatac ttaacccggc agtcatccat
     5281 aaatacgcgt gcgatgagat aaccgttgcc gcagcagcga ccaacagacc aatcataatc
     5341 ggccagccgc ccataattat cagcgaacgt acggtgcggc gcgaggtcaa acgcgctaac
     5401 agcaagttac ccgcaattaa cgccccgaaa ataggcactt gcagcaagcc atattcatag
     5461 ctgctcaact gctcgccggt aatgatgata atcggcgact gggcgatcca cgccagcaat
     5521 ggcaggctaa cgaatcccag cgccagcgcc cccgccacaa agcggccgtt cttcagcacc
     5581 agcttatagt cacgaccgag ttctttcagt gacagtttct cgcctatacg cgtggcggtt
     5641 tcaggcatgg ctcgttgcag accgaaaaag gagatcgctg ccaatgcggc aaacaaaaca
     5701 aacatcccct cccagggcag cacatggatc cacgccgcgc ccaccagcgg accaagtagc
     5761 ggagcaatca gcgccacgtt cgccatcagc gcggtgatct tgatacaaac cgcctcttcg
     5821 aaggattcct gaattgcggc gtatcccaca gcgccaatga aacagaggct tatgccctgc
     5881 aagaagcgca acagggtgaa ttgttcaatg ttttgcgcca gcaatattgc cagacaggtg
     5941 atgataaacc acaccacccc cgccagcatc accggacggc gaccaatacg atccgacagc
     6001 ggccccagga gccattgtaa aaacatcccg cccgccagat atgcggtcat tgaagtagga
     6061 acccaatcaa tgcccgcctg atattgttcc accacggcca acataccggg ttgaatcata
     6121 tcgttgccga tataggttga aaattcgtaa agcaccagac agagagggaa aagtaacgcc
     6181 tgacgtccaa gcctggcacc ggaagctaat ttattttgca tgcaatttct tcgccaataa
     6241 taatcgcgca gagtttaata aaagcacagc taacgagaaa gcgaattttg tagctgaaac
     6301 cacggttaag cacattctta cattattgcg agtatagcta cgctttcttt aagttttatt
     6361 taacctatgc ccgttacaat tacccaccgt aaacaggccg cttgagggaa ataagacgat
     6421 gccgctttac ccagtttaac ctgcacttta ttctcaacga cttgcctgta ttggctccct
     6481 tttaatcact ttgcgtcggg aagttatgct ggaaaatttg aatctctctc tattctctct
     6541 tattaacgcg acgccagact cggctccgtg gatgatctcg ttggcgattt ttattgctaa
     6601 agatttgatt accgtggtac cgctgctggc cgcggtgctt tggttgtggg ggtttacagc
     6661 acaacggcaa ctggtgataa aaatcgctat cgcgctggcg gtcagcctgt ttgtgtcctg
     6721 gacgatggga catctttttc cgcacgaccg accctttgtc gaaaatatcg gctataactt
     6781 cctgcatcat gcggcggatg actcattccc aagcgatcac ggtacggtga ttttcacctt
     6841 tgcactggca tttttatgct ggcatcgcct gtggtccggc tcacttttaa tggtgctggc
     6901 cgtcgtcatt gcctggtcgc gcgtttatct tggcgtccac tggccgctgg atatgctcgg
     6961 tggattgctg gcaggtatga ttggctgcct tagtgcccag attatctggc aagcgatggg
     7021 gcataaactc tatcaacgtc tgcaatcgtg gtatcgcgtc tgttttgcat taccgatccg
     7081 caaaggctgg gtgcgtgact gatctctgct aaaaagtgta gtattgagcg gctcgcttca
     7141 ataactattc agagggatta tggaaacacg tcgcgaagag cgtatcgggc agctgctgca
     7201 agaattaaaa cgcagcgata agttacatct taaagacgcc gccgccctgc ttggggtttc
     7261 ggagatgacg attcgtcgcg atctgaacaa ccacagtgcg cccgtcgttt tgctcggcgg
     7321 ctatattgtt ctggaaccgc gcagtgccag ccattacctg ttaagcgatc aaaaatcccg
     7381 cctggtggaa gaaaaacgcc gggcggcaaa actggctgcg acactggtag aacccgatca
     7441 gaccctcttt tttgactgtg gcaccaccac gccgtggatt attgaagcga ttgataatga
     7501 aatccctttt accgccgttt gttattcgct aaataccttt ctggcgctga aagagaaacc
     7561 ccattgccgc gcatttctgt gcggtggtga atttcacgcc agcaacgcca ttttcaaacc
     7621 catcgatttt cagcaaacgc tgaataattt ttgcccggat atcgcttttt attctgcggc
     7681 gggcgtgcat gtcagtaaag gcgctacctg ttttaatctt gaagagttgc cggtaaaaca
     7741 ctgggccatg tcgatggcgc aaaagcatgt gctggttgtc gaccacagta aatttggcaa
     7801 ggtgcgtccg gcgcgcatgg gtgacctgaa acgctttgat attgtggtga gcgattgttg
     7861 cccggaagat gagtatgtga agtacgcgca gacgcagcgc attaagttga tgtattaatg
     7921 acgtataacc ggatgacgtt tcgcgccatc cggttatcag aagattaaga gaaccagctg
     7981 ccgaaccact gatggaattt catcatcacg aaatcccaca tccgaccaaa gaatccgccc
     8041 tcttccacat tttccatcac gatcagcgga cgctgctcaa tggatttacc gttaagctgg
     8101 aaatcaatgg tcccgacaac ctgacctttt ttcagcggtg cggtaagctg cggttccgtt
     8161 aacgtataac tcgctttcag gtttttcagc tgcccacgcg gtatggtcac tgagcccgct
     8221 tcacctgccc cgagattcac ttcgctctta tcaccaaacc agacgcgctg agtcacaaag
     8281 gtggcatcag gtttaattgg cgtcacggtt tcaaagaagc ggaaacccca ggtcaataat
     8341 ttctcagact cattaaaacg gatacggtca gttttcgccc ccagcactac ggagattaaa
     8401 cgcatatcgc cctgggtagc cgaagcaacc agattatatc ccgcgcctgc cgtggttcct
     8461 gtcttcatgc catcaacatt cagattgctg ctccacagca gacggttacg gttaggctga
     8521 cgaattttgt tgaaggtgaa ctctttctct ttatgaatgg cgtactcttc cggcacatcg
     8581 tggatcaatg ctttacccag caatgccata tcgcgcgcgg tactgaactg ccccggcgca
     8641 tccagaccgt gcaccgtctg gaaggtagtg ttggtcagac ccagtttttt ggcataacca
     8701 ttcatcaaac caatgaatga ctcctggctc ccggcaacgt aatcggccag cgcaatacag
     8761 gcgtcattac cggactggat aatcacacct ttgttcaagt ctgccaccga aacctgatcg
     8821 cccggtttga ggaacattac cgatgaacca cgcagtgccg gatttcccgt cgcccaggca
     8881 tctttaccga ccgtcaccat atcggtgagt ttaatcttat cggccttaag cgcctgccca
     8941 accacatagc tggtcatgat tttagtcagg ctcgcgggat ccagtttctc atccgcgttg
     9001 ccttctgcca gcactttacc gctggcgtaa tccattaaaa tccatgcacg cgcatccacg
     9061 ctcggcgctt caacggtttg ttccgccgcg aatgccgttg gggcaaaaag gaataaaaat
     9121 gcagaacccg ctgcaagacc acgaaggaga gaggagtatt gcgtcataaa tgccacccga
     9181 gtatccattc aaaaaaaata cgttatatcg ccgtatgata agttcggtga ttaataacgc
     9241 acacacaaac ttaaagaaac cgtaagttgg taaagttttt aaagtttatg caatatcctg
     9301 ccgcttcgct gcttctggtg tcattttttt gccagctgtt gcttaaaagt taactttact
     9361 tcaccatcgg agtcctgttc agcctgtggc tgcctgtcgg aaaaggggtg agtatgatta
     9421 cgctgtgggg tcggaataat tcaaccaacg taaaaaaagt attgctgacg ctcgaagaac
     9481 tggaactacc ttatgagcaa attctcgcgg gccgtgagtt tgggataaat cacgatgctg
     9541 attttctggc gatgaaccct aacgggctgg tgccgttgtt gcgtgacgac gaaagtgatc
     9601 tcattctttg ggaatcaaac gccattgtcc gctatctggc ggcacagtac gggcaaaaac
     9661 gtctgtggat cgactcaccg gcacgtcgtg cggaagcgga aaaatggatg gactgggcaa
     9721 accagacgct cagtaatgct catcgcggga tcctgatggg attagtcaga acaccaccgg
     9781 aagagcgcga tcaggccgcc attgatgcca gttgcaaaga gtgcgacgcc ctgtttgccc
     9841 tgctcgatgc ggaactggca aaagtaaaat ggttctctgg cgacgagttt ggtgtgggcg
     9901 atatcgctat cgcaccgttc atctacaatt tgtttaacgt tggcctcacc tggacaccgc
     9961 gtccaaatct gcaacgctgg tatcagcaac tcactgaacg ccccgcagtg cgcaaagtgg
    10021 tgatgattcc cgttagctaa ttgcgtgggc taactttaag taattcccca ctggactcgt
    10081 cggtgagaac gtataaataa ccgtcgggtc cagtgcgaac atcacgaatt cgctgccctc
    10141 tgtccgttaa aatacggcca tcttctgtca ctttgtcgcc gttgacgctc atcacaatga
    10201 catctttatc tttcagcgcg ccaataaata atttttgctg ccactggggg aatttatcgc
    10261 tgttatagaa ggccatgccg ctcacggcgg gcgaatcttt ccagtaaaaa acaggttgct
    10321 cggtcccggc gacgatctcc cctttcgctt ccggtatctt aaagcctgaa tagttgattc
    10381 cccaggttgc cagcggccag ccgtagtttt tgcctttttg cgggatatta atttcatcac
    10441 caccgcgcgg gccatgttca ttcagccata gtgcattact ccacggattc atcgccattc
    10501 cttgcggatt acgaatgcca taagaccaga tctcggcgcg cgcaccggat tcctttataa
    10561 aaggattatc atccgggatt tcgccctggt cggtcagacg caccagtttg ccctgtaatt
    10621 tatccagatc ctgcgccgtc gggcgctgat tgttttcgcc cagagcaaca aaaagataac
    10681 ctttaccgtc gaataccagc cgcccgccaa aatggttgcc ggtagacagt tttggcatct
    10741 ggcgaaagac ggtgcggaag tcggtcactt ttgagagatc atcacttaag cggccataac
    10801 ccacagcagt tccggctttg ccatcatcgc caacttcgga ataacttaac cagatgcggc
    10861 gagactgagc aaaatcaggc gctaaaacca cgtccagcag gccgccctgc ccgtgcgccc
    10921 aaacgtccgg aactccggaa agcggcgcag ataatccttt tcctgcttgc cagtgacgca
    10981 actcgccgcc gcgcagagtg attaacatac cgtgattatc gggtaaaaag gccagtgccc
    11041 agggatggtc gagtttgtct tgcagtactt cgacatttac cgttgcagga gccgcccaga
    11101 gagcggaaga aagacaaata aggggcacaa ggaaaaagga ttgtcgatgc ataacgcctc
    11161 cggattgact ctggcttaaa gcgtagtcag tggaggagat aacaaattca tttttacaaa
    11221 aacttaaaca tgaaggggga gacgctttct cccccttagt tttcaggcct tctcaagcat
    11281 ggcgtgcttc tgcaggctct ggatactcag cgttaagctc atcagacaat tttcaagctt
    11341 atcggcgttg acggtaataa cagtcgggca atcatggtgc ccactcatca aacatactgc
    11401 ggctgtcgct aatgcttctt cagcatgatg aagagcactc cactcttcct gatccagatg
    11461 aagattcaac cgcagcgatt tatcgtgcag ttcgcgattc agtttaaaaa agttatctcg
    11521 tagatgattg ctttcgctga cggacatgta tccttttgcc ttcctcagtt gcacgtatgc
    11581 agcgaggtca acgcgtgcgt cgatcaaccg gttcagcaga tcgattcgct gtctgtcaac
    11641 gaacatactt cgatcctcct ctttccagcc ttgttgcaat taaatagtat ggctattttt
    11701 tgagcaaaat gctgtccagg atcaattctt cacgccgaat taacaaaatt tacatttcct
    11761 tcgcctccgc cagcgaatga gattgcgata caaattttat ccagaactct gtagaatccc
    11821 tgccctgacc tttcctgtaa ctgaatattt tttaagctat gagcaaagta actccccagc
    11881 cgaaaatcgg ctttgtttcc cttggctgtc cgaaaaacct tgtcgattca gagcgtattc
    11941 tcaccgaact acgcactgaa ggttatgacg tggtaccgag ctatgacgat gcggacatgg
    12001 tgatcgtcaa cacctgcggc tttattgaca gcgcggtaca agaatcactg gaagccattg
    12061 gtgaagcgtt gaatgaaaac ggcaaggtaa ttgtgaccgg ttgtctgggg gcaaaagaag
    12121 atcagatccg cgaagtccac ccgaaagtgc tggaaatcac cgggcctcat agctatgagc
    12181 aggttctgga gcacgttcat cactacgtgc caaaaccgaa acacaaccca ttcctgagcc
    12241 tggtgccaga acaaggtgtg aaactgacgc cgcgtcatta tgcctatctg aaaatttcgg
    12301 aaggctgtaa tcaccgctgc accttctgca ttattccgtc tatgcgcggc gacctggtga
    12361 gccgtccgat tggcgaagtg ttaagtgaag cgaaacgtct ggtagatgcg ggcgttaaag
    12421 agattctggt gatctcgcag gatacttccg cctatggcgt tgatgttaaa catcgtactg
    12481 gcttccacaa cggcgagccg gtaaaaacca gcatggtcag cctgtgcgaa cagttatcga
    12541 aactggggat ctggacacgt ctgcactacg tttaccctta tccgcatgtg gacgacgtca
    12601 tcccactgat ggcagaaggc aaaatcctgc cgtatctgga cattccgttg cagcacgcca
    12661 gcccgcgcat tctcaaactg atgaagcgtc cgggttctgt agatcgccaa ctggcgcgca
    12721 tcaaacagtg gcgcgaaatc tgcccggaac tgaccctacg ctcaaccttt attgtcggct
    12781 tccctggcga gacagaagaa gatttccaga tgctactaga cttcctgaaa gaagcgcgtc
    12841 tggatcgcgt tggctgcttt aaatacagcc cggttgaagg tgcagacgcc aatgccctgc
    12901 ctgaccaggt tccggaagaa gttaaagaag aacgctggaa ccgtttcatg cagctgcagc
    12961 agcagatttc cgccgagcgc ctgcaagaga aagtgggccg tgaaattctg gtgattatcg
    13021 acgaagtgga cgaagaaggc gcgattggtc gcagcatggc agatgcaccg gaaatcgacg
    13081 gcgcggttta tctcaatggt gaaaccaacg ttaagccggg tgatatcctg cgtgtgaaag
    13141 tcgagcacgc cgatgagtac gatttgtggg gtagccgggt ttaattaatt caggccgggc
    13201 aaaatcattt gtccggcctg cgaataatga ttactgccag ctaatctgag ccctgccgtt
    13261 tttcttactc ttatacagcc gctcatccgc cagcattttc aagcgttcga cggtggtaca
    13321 ttcatttgaa tgcgctacac cgatactggt acttaaatta atgcttccca cctggaaatg
    13381 aacgatttct tttagaatcg atagcaactc ttccagcgtt ccacccgtat aaatcaccgc
    13441 aaattcgtca ccgccgaagc ggtagatttt acctttatct accgggatct gtgatttaag
    13501 gattttcacc actttcatca acacttcatc gcccagcaaa tgaccgaagg tgtcgttaat
    13561 gtttttgaat ttatcgacgt caaacaggca caaataaccg cctttttccg gcagcgcttt
    13621 taaatcttcg tcaaaggatt ttctgcccat tgctttggtc aggtcgtcca ggaacgccga
    13681 gttatacata ctgtagagtt ttttgacatc ggcagccgaa aagaaaagcc cgccatccag
    13741 tacgcgttca aaatggttat taatgatggc ttctttctcc acaataccac gattaatcac
    13801 tctgcgtacc agatagaggc aactggtacc cgatatcact gccaccaatg cggcaaagaa
    13861 aataatcaat gtacagaccc gcaaataaac atctttttta tacgcaaata cgtacaatcc
    13921 ggtcggcaaa taggcgaagt gctccatcgc ttccgaaacg gtgaagatgg cgtttttagc
    13981 attatattca tcaccggtca ccggtttacc gttcttaaat agagagaaca tcaggttctc
    14041 gctgtccatc gccagcgatt ttgacagtaa agaaaaacgc tccagaccaa gcaaaaatat
    14101 cagactacga ttgtgagtta ccggatagag gtaatagacg aaggttttac cgtcaaaaga
    14161 gataaaccct gtatttttga catgtcgata cttcacaacc tgcataatat ctgcacaata
    14221 atttttctcg aactccttta cctgaataca ttcgtcactg gaaatcagat aaggaccgac
    14281 atcgtacaaa taagaggggg aaaccgagtc ggcgtaagct gtgcgcaaac caaccaacag
    14341 ctgttcctcc gtgggattta gcgtgttgat aagatcaatt gccacgctac gccgatattc
    14401 agcaatattt gccaccgcaa aagcagtatc aataacggac tgtttcaggc tatacacccg
    14461 ttcctttacc atttgagtgt agatcccgca ggcaactgct gaaatcatga taaaaatcag
    14521 cagactgact gtaagtacga acttattgat tctggacata cgtaaactac tcttttacta
    14581 attttccact tttatcccag gcggagaatg gcatgggttt ctggtacaga tacccctgag
    14641 cctgaacaca tcctaattta attaattcgc gcgcaacgtc ttcgctctct acgccttccg
    14701 caatcaccgg gatccggtta tagcgtgcca gatcgaaaat aatgcgcaca atttttcccg
    14761 cagtaccgaa aatatcgccg gtaaattgcg agtccacctt tatgctgtct ggattaattt
    14821 cataaaaacg tttcaggttt gacaaaccgg tgccaaagtc atcaatcgat atccgtaccc
    14881 cttgtttacg cagttgttca atattgagat aaaaatgttc attaattcgc tcgatcacgg
    14941 tttcagtgat ctcaacacag aggctgttcg ccttcaggcc attttctttc atgatggtgg
    15001 taaaacgctc atgaaagtgc ggttcgttta actgccaggg cgaaacatta atatgcagta
    15061 agaaatcatc cttcagcgac gcacgctgac taaaatgtct gaattcaccc attgccgttt
    15121 taataatctg ataacccagc tcattgataa acccgatatc ttcagcaatg gtaatgaact
    15181 tcagcggtga aatgatcccc cgctccgcag aaacccaacg gcacaacgcc tccgcgccca
    15241 gaatggcgcg ggtgttaata tcaacgatcg gttgatagac gaggaatagt tcgccaattt
    15301 ccattgcatg gcgaatatta cgcgcaatcc agatattctc ttctatttcg ttgagtttcg
    15361 cgtcatcaca gaaaataaac tcggagaaca tatggtcacg gatactggaa atcgccagtt
    15421 tcagtttttc tgcgtaactc caggcgtttt ccccctgcaa aggttcacaa atggcagact
    15481 tatggatgcg cagattgcgg tagccaaagc tgctgtcaaa atcgtttacc atcgctattt
    15541 tacgggtaaa tgtctgtaca ttttcccgac acagaagtaa atagagatcg gcattgaatg
    15601 tcacgatatc gcagcattgc gcatatttct cggcaattcg ctgacacaat ttcctgacga
    15661 ttttattgcg ttcatcaaca ccaaacaccg cttccacggc ttccagacca cacagtttga
    15721 tgatccccac agccagatac tgattattgt tggactcaat aatcgccata tttttaataa
    15781 aaccgctttc ctgatcgtaa gagatcagtg agagcaattt atttgaccgt tcaatttcgg
    15841 catctttcat ttccgcgatc gaatttttga tgctgataat ctctttcgca tcgccttcgc
    15901 gaattttcag agcctcgatt tgatccgttg agtcgggcaa aaacgccagc gtattaacca
    15961 gctcggtcat ttcagaaaat tgccgacgga acacacgagt ggtcatgaaa tataccacca
    16021 gcaacacgat aataatgaaa gcaaacggta gataaaaaag tttatctgat gcgttcaata
    16081 aattaccgcc aggataataa aaatagattt cgaacaatgc attatttggc actgctgatg
    16141 tctttgcaat atactccgta tcgacagaat caataatatt ataattacct tcgctattat
    16201 aaataatcag tttttcacga gcgagttgat tcttggcaat gatctcatga ccttgtttca
    16261 aaaatagaac ttcatcatta gcgctgattt gatcaagata ctcgttcaga taccgccagt
    16321 ttaaatcgac atgaatttta tctaccgcta acaaatcgtg atctttgccg ttgatacgta
    16381 gcgtaaaaga gatacatttt ttacccggac gatcaaagca attgtatgcc ggattccagc
    16441 tgttgttaga gtcatctgaa gccaccgacc acggtcgttt cgatgggtta aaatcggcaa
    16501 aaataacccg ccctgactcg tcgacaagcg gtccatttac ttgttcctga tgataaacaa
    16561 cgattttatc attcgccctc cgcgaaaaga gataatattt atta
//
LOCUS       DAZGVQ010000113        16593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000113 DAZGVQ010000000
VERSION     DAZGVQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.114"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..97)
                     /locus_tag="ACHLAG_003558"
     CDS             complement(<1..97)
                     /locus_tag="ACHLAG_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656718.1"
                     /translation="MALTHHELCQIAYKFLKRNGFKVCFHDRFVAV"
     gene            complement(99..536)
                     /locus_tag="ACHLAG_003559"
     CDS             complement(99..536)
                     /locus_tag="ACHLAG_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP0656719.1"
                     /translation="MSKIDYQALREAAQNYQSTLAWYQEKPDSPNAEQDCDAALAAFK
                     RHIRHREADIIADLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELEAREVQLPTRYD
                     LRYGHPINADERQVMIPKENGSWLYLIDLEHALRVAGIRIKGE"
     gene            complement(523..840)
                     /locus_tag="ACHLAG_003560"
     CDS             complement(523..840)
                     /locus_tag="ACHLAG_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656720.1"
                     /translation="MTITKQRVEEIISRIGMYGHGAGYTADEVNDLAVLALNLSNIAN
                     LKRYELDMGGCDSCGQDCGTDMTEDPDGDYVLFDDVVKLFEFDTTTQKLEIPAKEAAS
                     EQD"
     gene            complement(752..1111)
                     /locus_tag="ACHLAG_003561"
     CDS             complement(752..1111)
                     /locus_tag="ACHLAG_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFP0656721.1"
                     /translation="MNIDPAITIDTALNTGLALLGYFYIMFCSGRWMSLLFMKKWNKR
                     RRQDQRQQAMDAFFEAFGIDGMEPGDPARAISRGGVVILVYRSEEKNDDHKTTCRRNH
                     IPHWNVWTWCRVYRRRG"
     gene            complement(1101..1397)
                     /locus_tag="ACHLAG_003562"
     CDS             complement(1101..1397)
                     /locus_tag="ACHLAG_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP0656722.1"
                     /translation="MRIYIAGPMTGYENFNREAFHKAAEALKREGHTVLNPAVLPDGL
                     TQPHYMDICMAMLRCVDAVYILKGWQQSAGARTELALTEKLGHAVIFQEVVSEY"
     gene            complement(1394..1711)
                     /locus_tag="ACHLAG_003563"
     CDS             complement(1394..1711)
                     /locus_tag="ACHLAG_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0656723.1"
                     /translation="MSGWKKWRWAEILILRQCAGTMRVESIGYLIGRSESAVRTKARE
                     LGISMMLRGDYHQSAKFSQRDIELARQLHQRGVPQREIAEKFGMKLGAVNNYVYFDRR
                     VQE"
     gene            complement(1708..2424)
                     /locus_tag="ACHLAG_003564"
                     /pseudo
     CDS             complement(1708..2424)
                     /locus_tag="ACHLAG_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309538.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            complement(2446..3192)
                     /locus_tag="ACHLAG_003565"
                     /pseudo
     CDS             complement(2446..3192)
                     /locus_tag="ACHLAG_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(3199..4167)
                     /locus_tag="ACHLAG_003566"
     CDS             complement(3199..4167)
                     /locus_tag="ACHLAG_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0656724.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAATAAR
                     EAYKPKRKISPAKNDPLTVNPSNIAPSTVDPSNVDGSTVDKKLRIRGAMIDPDPSVLK
                     PDPSDKRSSCPDVSLLDEKRSSPVERFLEKHPDAHTWNVPKRQWGTREDVACAQWIWG
                     RVVALYEQAASDDGEVSRPREPNWTTWANDVRMMRMLDGRSHRQICEMFGRVQRDSFW
                     VKNIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(4190..4615)
                     /locus_tag="ACHLAG_003567"
     CDS             complement(4190..4615)
                     /locus_tag="ACHLAG_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP0656725.1"
                     /translation="MKIKHEHIRMAINAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(4599..4880)
                     /locus_tag="ACHLAG_003568"
     CDS             complement(4599..4880)
                     /locus_tag="ACHLAG_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFP0656726.1"
                     /translation="MEELRIFLNSLSSDEQRMFACECGTSIGYLRKALSKGQVLGASL
                     CVLIERASNGEVTRQQLRPFDWMNIWPELEDTKTLTQPLSRSLIHENQA"
     gene            4981..5400
                     /locus_tag="ACHLAG_003569"
     CDS             4981..5400
                     /locus_tag="ACHLAG_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656727.1"
                     /translation="MDKYEFRRQQLIKIRDEKCDGKAVNVARKIGREPSYVSRMLYPE
                     GKKGKKRIADDMVEIIEESFGLPRGWMDGIVSSSTNTASNYETRVLTPRQRIFLDLLD
                     ELPESEADNLLKTLEEKKQYYNMIYEEIRKKKAQNAS"
     gene            5667..5819
                     /locus_tag="ACHLAG_003570"
     CDS             5667..5819
                     /locus_tag="ACHLAG_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP0656728.1"
                     /translation="MDTIELGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGAHRW
                     LARNAN"
     gene            6292..6480
                     /gene="dicB"
                     /locus_tag="ACHLAG_003571"
     CDS             6292..6480
                     /gene="dicB"
                     /locus_tag="ACHLAG_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP0656729.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNKAFTEDAINMRKYEPYLLNDN
                     SILSRIALIKLGIFGERQ"
     gene            6537..6665
                     /locus_tag="ACHLAG_003572"
     CDS             6537..6665
                     /locus_tag="ACHLAG_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP0656730.1"
                     /translation="MITGIYASKESCLQARDEQKISGECLPVKKVSLNLNNETPAG"
     gene            6761..9232
                     /locus_tag="ACHLAG_003573"
     CDS             6761..9232
                     /locus_tag="ACHLAG_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP0656731.1"
                     /translation="MNKIFICAAIPDEQAIEEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPSIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPM
                     NVTFDNLAPEVQNAVMVKFDACENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPAAKWPEIQAEMRIWKKRRESERKETGKYISVVDIARS
                     RVNQQNTENAAEKTGAVTVAVRREYKQTWKTLDNELACALWPGDVDAGNIDGTIHRWA
                     TNEVIDKDREDWKRISASMRKQPEALAYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSADALQSPAAQTAPVETAESDTQKNEILVEAEPSVEREGPFYF
                     VFTDKGGEKYGRANKLSGLDRALAAGGTEISKEEYFARKNGTYTGLPQNVDSTEDSEQ
                     PEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPDDPKWVKGIQSRDAE
                     DQNQPNVKQNEPEAEQNSPDTQQNGPEEQQPEPAVQQELEKVCTACGQTGGGNCPDCG
                     AVMGNATYQETFDEENQAEAQENDPEEMEGTEHLHKENTVSDQYHASDNKTGETANPL
                     IKVNGHHETTSTSRLWHHLMIDLETMGKNPDAPINAIAGKFFDPATGEMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAILTDEIPLDDALLQFREFIDENSGEFFVQVWG
                     NGATFDNVILRRSYERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGERHNA
                     LHDARYQAKYVSAIWQKLIPSQADF"
     gene            9297..9545
                     /locus_tag="ACHLAG_003574"
     CDS             9297..9545
                     /locus_tag="ACHLAG_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP0656732.1"
                     /translation="MARLILLTEWAKEEFSDPVPTPGTLSKYAKAGMIFPLPKKVGRH
                     WRVDPRARFVGMVNKPEVIATDHPALKRILEDGAPTKI"
     gene            9523..10653
                     /locus_tag="ACHLAG_003575"
     CDS             9523..10653
                     /locus_tag="ACHLAG_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFP0656733.1"
                     /translation="MARPRKYKTEVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKQAEAIAAEANSRLARQQMEQMLSLQEKIISDTGSPSTVSAFLNSYRKIQQERYENG
                     EIKLNTLKQKASPLRVFDERFGTRPLDAITVKDVVSVLEDYKAKGHNRMGQIFRKVLI
                     DVFREAQQTGDVPLGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMKFSDIRDGYLHVEQQKTGTRIAIPLALRYDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGIDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(10699..11352)
                     /gene="ompW"
                     /locus_tag="ACHLAG_003576"
     CDS             complement(10699..11352)
                     /gene="ompW"
                     /locus_tag="ACHLAG_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFP0656734.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTMAKYKSDGRKLAKGSYSDNVRLDPWVFMFSAGYRF"
     gene            11592..11681
                     /locus_tag="ACHLAG_003577"
                     /pseudo
     CDS             11592..11681
                     /locus_tag="ACHLAG_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            11709..12452
                     /gene="yciC"
                     /locus_tag="ACHLAG_003578"
     CDS             11709..12452
                     /gene="yciC"
                     /locus_tag="ACHLAG_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFP0656735.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            12482..13021
                     /gene="yciB"
                     /locus_tag="ACHLAG_003579"
     CDS             12482..13021
                     /gene="yciB"
                     /locus_tag="ACHLAG_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFP0656736.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            13126..13524
                     /gene="yciA"
                     /locus_tag="ACHLAG_003580"
     CDS             13126..13524
                     /gene="yciA"
                     /locus_tag="ACHLAG_003580"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFP0656737.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(13564..14283)
                     /gene="tonB"
                     /locus_tag="ACHLAG_003581"
     CDS             complement(13564..14283)
                     /gene="tonB"
                     /locus_tag="ACHLAG_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFP0656738.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            14507..14803
                     /gene="yciI"
                     /locus_tag="ACHLAG_003582"
     CDS             14507..14803
                     /gene="yciI"
                     /locus_tag="ACHLAG_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFP0656739.1"
                     /translation="MLYVIYAQDKADSFEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            15102..16355
                     /gene="kch"
                     /locus_tag="ACHLAG_003583"
     CDS             15102..16355
                     /gene="kch"
                     /locus_tag="ACHLAG_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFP0656740.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(16410..16583)
                     /gene="yciY"
                     /locus_tag="ACHLAG_003584"
     CDS             complement(16410..16583)
                     /gene="yciY"
                     /locus_tag="ACHLAG_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFP0656741.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
BASE COUNT         4333 a         4134 c         3813 g         4313 t
ORIGIN      
        1 ttacagcaac aaagcggtca tgaaagcaaa ccttgaaccc gttgcgctta aggaacttgt
       61 acgcaatctg acagagttcg tggtgtgtta acgccatatc actctccttt gatgcgaatg
      121 ccagcgacgc gtaatgcgtg ttctaggtca atcaggtaaa gccaactgcc attttcttta
      181 ggtatcatga cttgtcgctc atctgcattt atcgggtgtc catatcgaag gtcgtaacga
      241 gtcggtaatt gaacttcccg cgcttccagt tcagcaatac gcttgctccc atcagagata
      301 acgccttcgt aatactcacg ctgctcgttg agttgtgatt ttgcttcttc cagtccatcc
      361 agcaaatcag cgataatatc cgcttcccga tgacggatgt gacgcttaaa cgccgctaaa
      421 gctgcatcac aatcctgttc agcgtttgga ctgtctggct tttcctgata ccacgccagc
      481 gtcgattgat agttttgtgc tgcctcacgc agtgcctgat agtcaatctt gctcactggc
      541 tgcctccttt gccgggattt ctaacttttg agtggttgta tcaaattcaa acaacttaac
      601 cacgtcatca aacaggacat aatcaccatc aggatcttca gtcatatcag tgccacaatc
      661 ctgaccgcac gagtcgcaac cacccatatc aagctcgtat cgcttcaggt tcgcgatatt
      721 tgataaattc agcgccagta cagccagatc attaacctcg tctgcggtat accctgcacc
      781 atgtccatac attccaatgc gggatatgat ttcttctaca cgttgttttg tgatcgtcat
      841 ttttctcttc actccgatat acaaggatta ctacgccccc tctgctgatt gcgcgagctg
      901 gatctcctgg ttccataccg tcaatcccga aggcttcgaa aaatgcatcc attgcctgct
      961 ggcgttgatc ctgcctacgg cgtttattcc attttttcat gaacaacagt gacatccatc
     1021 gtccgctgca gaacatgatg tagaaataac caagaagtgc caggccggta ttcagggccg
     1081 tatcaatcgt tatcgccggg tcaatattca ctgaccacct cctgaaaaat aaccgcatga
     1141 cccagtttct ccgtcagtgc cagctcagtc cttgcacctg ccgactgctg ccagcctttc
     1201 agtatgtaaa ccgcatccac gcaacggagc attgccatgc aaatatccat gtagtgtggc
     1261 tgagtcagcc cgtccggaag cacagccgga tttaaaacgg tatgcccttc ccgtttcagc
     1321 gcctctgccg ccttgtgaaa tgcctcgcgg ttaaaatttt catatcccgt catcggaccg
     1381 gcgatataaa tcctcactcc tgaaccctcc tgtcgaaata aacgtagtta ttcactgcgc
     1441 ccaacttcat cccaaacttt tcggcaattt cccgttgggg tacgccacgc tgatgcagtt
     1501 gccgcgccag ctcaatatca cgctgtgaga atttggctga ctggtgataa tcaccccgta
     1561 acatcatgct gatccccagt tcccgcgctt tcgtcctgac ggctgactca ctacggccaa
     1621 tcagataacc gatgctttcg actctcatcg ttcccgcaca ctgccggagt atcaggattt
     1681 cagcccaacg ccacttcttc cagccactca ccgctgctgc tctctggtgg tggtaatatc
     1741 ccggagaata tccctgtgtt tgttcagttc ccgcagcgca gcacagactc gctcccactt
     1801 ctggacatca cttttcgccc gacgcagttc gcggtttgcc atatgcagct atggtaaaac
     1861 caggtcatcc gcttgcgttt cggtgaacag tggcaacgac tgcacaatat ccaccacagt
     1921 ttctgtttta atatcttcct gtgtagtcgt ttgctggact ggtaacgcaa cacctgctgg
     1981 ctgaggaagg gctttaccat ccgtttccgc tacagatgcg gctttcggct ctactggtaa
     2041 attatcgccc ggcatgcagt aacgaaattt accgccctga ttcacgcgaa tcagacgccc
     2101 tttgctgatt gccattgcca gcgatgaatt cgcacggcgg gaggtaattc cgaacatcaa
     2161 tgccagctca tccgccgttt gtgggccatg ttgttcaatc gcctctgtca gcatttgcgc
     2221 cgtcactttt ggtggttgta tcacagattc actttcccct gcctgagtca gccaccacat
     2281 cgcccccctg ttatccgctt caccacggcg tttcagtttc cacagttcgt tgacagcctc
     2341 ttcacgactg attccaaggc gcgcagccac cacatgtgaa gaggcttttt tcagtgcttt
     2401 cagtgcgtca gatacggttt ccattaaaat ttcctccgga caaaattact tcacaaccct
     2461 catattgctg acatttggac gccagctatc ccagttaaac gtcacccatc gaccaccgtt
     2521 catggtcatg cggtccataa tcctctcacc aagaagcgta ctcattgcgg catgattcag
     2581 gtttgttaac atcccgacac tgcacagtga tgctgtccgg cgatcaatta tctggtgcaa
     2641 taccacctgc tcgtttttcg tctcccgctg gacgcctatt tcatccagga ccagcaaatc
     2701 aaccccgcaa agctcctgta aaaatttttc cccggatttg ccgttgtcgt agctgtcatg
     2761 caacacgctc atgacgtcag acacggtgac gataatcacg ctgcgcccct tcaccatcag
     2821 ccggttgccc atcgccgctg caaggtgatt tttcccggtg ccggttttac cgctgaacac
     2881 aaaattcgtg cacccggtca tcagttcgtc agctatggat ttggcctagc tcagcgcgta
     2941 tttttgcccg tcgttctgca cctgataatt tgcaaacgag catttgctgt gcagaggctg
     3001 gatgcccgaa cgattcagga ttttttccac ccgcaactgg cgattctggc agttaatctc
     3061 ctcgctgcgt tttcgtcctt cagcaagttg ccattcccgc cactcctcca ccgtccggta
     3121 cggtggaacc gcccccagtg gtgcaagtct gcgaatacat tcaagaaccc caactgccgc
     3181 aatgtttttc atgccacatc accccctgaa tcccggcggt atttcagtgt ccggttcaga
     3241 aatgtgattt acgcaacgct gcgcgggcga acgccccagg cggataacca gttcatccca
     3301 tttttccctg agttttgccg gactcatgat gttttttacc cagaacgaat cccgctggac
     3361 acgcccaaac atttcacaaa tctgccggtg actacgtcca tccagcatac gcatcatgcg
     3421 cacatcattc gcccaggtcg tccagttagg ttctctgggg cgtgacacct caccatcatc
     3481 actggcggcc tgttcataca acgccacaac ccgtccccaa atccactgcg cacaggcgac
     3541 atcctcacgg gtaccccact gtcgcttcgg tacattccag gtatgcgcat ctgggtgttt
     3601 ctccagaaat cgctcaactg gtgatgatcg tttttcgtcc agcagtgaaa cgtccggaca
     3661 agaagatctt ttatctgacg gatcaggttt taatactgac ggatcggggt caatcatcgc
     3721 ccccctaata cgcagttttt tatcaaccgt tgatccatca acatttgacg ggtcaaccgt
     3781 tgagggggca atatttgacg ggttaactgt taacgggtca ttttttgccg ggctaatttt
     3841 tcttttcggt ttatatgcct cacgcgccgc agttgcagct gcttcgagtt tttccacatt
     3901 aagccgatag atattgctta cgttacgccc accgacctta cgctcttcct tcgtcagcca
     3961 gccctctttc gccagttctg caatagcaga tttaacggtg gattcacttc ttgcaccgat
     4021 ctgacgccgg atagtttcaa tggcaggcca tgacacgccc tcgtcattgc tgtagtctgc
     4081 aagacgggcc ataaccgcca ccctggataa gatcatgccg gtgaaggcgc acccttccca
     4141 gacaagacca tgaagcttgc tgctcataaa ccccccgaac accgtgcttt tagtgcatca
     4201 ccacagcatt ccctgccggg ccgccgcgat tcatctggtc atacaaaaca accgctgacg
     4261 caacaaaatc atcgacatcc ttcaccagcc gatccctccg ttcgacgatc tcacggtaat
     4321 attcagaact gtggctgcgc atacgggcca ccagcaaagg cggcatcgcc ttttcgatcg
     4381 ccggtaacag agcctgcatt ttttcaacag catcaggggt gtctttatcc agccaacgga
     4441 aaattttctg tgtattacgg gccagggctt ccggatggct gtcgtcgtac agttcaggga
     4501 acgtcatccc cagttcgaaa taagtccggg ctatttcagc tgcaggaact ttctcaccgt
     4561 ccggataggc ccaggcattg atcgccatgc ggatgtgctc atgcttgatt ttcatgaatc
     4621 aagctcctag aaagtggttg tgttaacgtt ttggtatctt ccagctcagg ccaaatattc
     4681 atccaatcaa aaggccttag ttgctgacgt gtaacttcac cattactggc tcgctcaata
     4741 aggacacata acgatgcccc taacacttgg cctttactca atgcctttct tagataaccg
     4801 atgctggtac cacactcgca tgcaaacata cgctgttcat ctgacgaaag agaattgaga
     4861 aatattctta attcttccat agctactcct tagtaaacac agcaaagaat acctataggt
     4921 aaacaaaagt caataccttt gggttgttta ccttacggta atcacatcta ttatttacct
     4981 atggacaaat atgaatttag acgacagcag ctcatcaaaa ttcgtgatga gaaatgcgat
     5041 ggtaaagcgg ttaacgtagc cagaaagatc ggtcgcgagc cttcttatgt atcaagaatg
     5101 ttgtacccag aggggaaaaa gggaaaaaaa cggatcgctg atgatatggt ggagattatc
     5161 gaagagtcct ttgggttacc ccgaggatgg atggatggta tcgtttcatc atcaacgaac
     5221 acagcctcca attatgaaac aagggttcta acgccacgac aacgtatttt tttagatctc
     5281 ttagacgagc tgccagaaag tgaagcggat aacttattaa aaactcttga agagaaaaaa
     5341 cagtattaca atatgatcta cgaagaaatc cgtaaaaaga aagcacaaaa cgcatcataa
     5401 ctcaccaaac aaccagtcac cagttaagac gcctgaaaaa aagttaccca tgggtattta
     5461 ctttttgggt acccatgggt atgctccctt tcataccaac ccactccgcc ccacagaatg
     5521 cagggcaata cctcgagtta cccggcagtg gtcaggggtt aagtagccag cccgaggcgt
     5581 atgaacatga cggcgggaac actttgtata acagcgcagc aggtttttag ttccgctacc
     5641 cggcgttaag ggtaaatggg gtcaacatgg atactatcga gcttggcaac aacgaatctc
     5701 tggtgtgcgg agtattcccc aaccaagacg gcacgtttac cgcgatgacg tataccaaaa
     5761 gtaaaacgtt taaaaccgaa gctggcgcgc atcgctggtt agccagaaac gctaactgat
     5821 tagcgccagt aaaaacaggt ttccacaggt taatttaccc tgaaaagtca gggcataaca
     5881 cgaaagcgca cggcgagatt cctttgcata taaggttaga tttcttcgac cgtgcgcttc
     5941 cggttgtggc aatccgcgaa atggcgcggc ggtaagtatg gcggggttat tccttccccc
     6001 gttgaggaca ccgggttgtc aggttgacca tacgcttaag tgacacccca gccacaacaa
     6061 cctctgttat cacttttctg gtgattcggc ggaaatgaat atccgccctt tttaaagtga
     6121 attttgtgat gctgtgaatg cggctatgcg cacgcggaac agttaaagca gtaaggcggt
     6181 cttttactgg cgtaacgagc atcaactaac ccgacgttaa ttgttaactg gttaacgtca
     6241 cctggaggca ccaggcgctg catcacaaaa ttcattgttg aggacgcgat aatggaaacg
     6301 ttattaccaa acgttaatac gtctgaaggt tgttttgata ttggtgttct gctcagtaat
     6361 aaagcgttta ctgaggatgc cattaatatg cggaaatatg agccttatct gctcaatgat
     6421 aattccattc tttcccgaat tgcccttatt aaacttggta ttttcggaga acgtcaatga
     6481 cttcagcatt tgcactggtg atgacggttt ttcttataac gggtgaataa cagaatgtga
     6541 ttaccggaat ttatgccagt aaagaatcct gcctccaggc aagagacgag caaaaaattt
     6601 ctggtgaatg cctaccggta aaaaaagtat cgctgaacct gaataacgaa acaccggctg
     6661 gataacccgc cagccatatt aacgccatac caacggatta aaaatgccag caatagcagg
     6721 gattcgttca ccctgaaatc tgtaatgagg ttaaaacaca atgaataaaa tctttatttg
     6781 cgctgctatt cctgacgaac aggccataga agaagatagc gctgttgcgg tggccactgc
     6841 cattgaagcc ggtgatgagc gtcgcgcacg cgcaaaattt cactggcaat ttctggagca
     6901 attcccggca gctcaggact gcgcttataa atttattgtc tgcgaggata aacccagtat
     6961 accccgccct gccctcgatt cctgggatac cgaatatatg caggaaaacc gctgggatga
     7021 ggagtctgcg tcctttgtcc cggtcgaacc cgaatccgat ccgatgaacg tcacttttga
     7081 caatctggcc cctgaagtac agaacgccgt tatggttaag ttcgacgcat gtgaaaacat
     7141 cactgttgat atggtgatta gcgctcagga attgttgcag gaagacatgg caacattcga
     7201 cggccatatc gttgaagcgt tgatgaagat gccagaagtt aacgccatgt atccggagct
     7261 taagctgcat gccatcgggt gggttaagca taaatgtaaa cctgctgcta aatggcccga
     7321 aattcaggca gagatgcgca tctggaaaaa acgtcgcgaa agtgaacgca aggaaaccgg
     7381 gaaatacata tctgtcgttg atatcgcccg ttccagagta aaccaacaga acactgaaaa
     7441 cgctgctgaa aaaaccgggg ctgtcactgt tgccgttcgc cgcgaataca aacagacatg
     7501 gaaaacactc gacaatgaac tggcctgcgc cctctggccc ggtgatgtgg atgcaggaaa
     7561 cattgacggt accatccatc gctgggcgac aaatgaggtt atcgacaagg atcgcgaaga
     7621 ctggaagcgt atctcagcat caatgcgcaa acagcccgaa gcacttgcct atgaccgtca
     7681 gactattttt ggccttgttc gcgaacgtcc gatcgatatt cacaaagatc ccgttgcact
     7741 gaacaaatat atcagcgaat acctgacgac aaagggcgtg tttgaacatg aagaaacaga
     7801 ccagagctct gctgatgctc tccagtcacc agcagcacaa actgctccag tggagacggc
     7861 agaatccgat actcaaaaaa atgaaatcct ggtggaagct gaaccatctg tagagcgtga
     7921 aggaccattt tatttcgtct ttaccgataa gggcggggaa aaatacggca gggcaaacaa
     7981 actttctggt ctggacaggg cgctggctgc cggcggtacc gaaatctcaa aagaagaata
     8041 ttttgcccga aaaaatggca catacacggg cttaccgcaa aatgtggata gcactgaaga
     8101 ttccgaacaa ccagagccgg taaaagttac cgctgacgaa gtaaacaaaa ttatgcaggc
     8161 agccaatatc agccagcctg acgccgataa attgcttgct gcatcacgtg gtgaatttgt
     8221 tgaagggatt agcgacccgg atgatccgaa atgggtgaaa gggatccaga gtcgcgacgc
     8281 tgaggaccag aatcagccca acgtgaaaca aaatgagcca gaagcggaac aaaacagccc
     8341 ggatacgcaa caaaacgggc cagaagaaca acaaccagaa ccagcagtgc aacaggaact
     8401 ggaaaaagtt tgcaccgcat gcggtcagac cggtggcggc aactgtcctg actgtggtgc
     8461 ggtgatgggg aacgcaacct accaggaaac attcgatgaa gaaaatcagg ctgaagctca
     8521 ggaaaatgat ccggaggaaa tggaaggcac tgaacatctg cacaaggaga acactgtcag
     8581 cgatcagtat cacgccagcg ataataaaac tggcgaaaca gcaaatccct taattaaagt
     8641 gaacggtcat catgaaacca catccaccag caggttgtgg caccatctga tgattgacct
     8701 tgaaacaatg ggaaaaaatc ctgatgcgcc aataaacgct atagccggta agttttttga
     8761 tccggcaacc ggagagatgg ggccagaatt cagcaaaact atcgatctgg aaaccgcagg
     8821 cggggtcatc gatcgggaca ccattaagtg gtggctgaaa cagtcacgcg aagcacaatc
     8881 cgccattctg accgatgaaa tcccgttgga tgatgcactg ctgcaattcc gggaatttat
     8941 cgacgaaaac tccggtgaat tttttgttca ggtctggggt aacggtgcaa ctttcgacaa
     9001 cgtgatttta cgccgttcat atgaacggca ggggatcccc tgcccgtggc gttacaccaa
     9061 tgatcgcgat gtaagaacga tggttgctct gggactggtg atggatttcg acgcaagaac
     9121 gactattcca ttcgaaggtg aacgccataa cgcgctgcac gatgcgcgtt accaagcaaa
     9181 atacgtttca gccatctggc aaaaactgat cccgagtcag gctgattttt aatgttcaac
     9241 cccggtcgtc gcccgcaagc tatagtggcg gcgaccatga ttagcgaaca actctcatgg
     9301 caagacttat tcttctcact gagtgggcaa aagaggaatt cagtgacccg gtcccgactc
     9361 cgggcacgtt aagtaaatac gctaaagccg gaatgatatt tcctctcccc aaaaaagttg
     9421 gaagacactg gcgagtggat cctcgagctc gctttgtcgg aatggtaaac aagccggaag
     9481 tgatcgccac agatcaccct gctttgaaga ggatactgga agatggcgcg cccacgaaaa
     9541 tataaaactg aagttccggg attatctccg tattttgaca aaagaaataa caaagtttac
     9601 tggcgttaca ggcatcccat aacaggaaaa aatcacggac tcggcagtat tgaccagaaa
     9661 caggcagaag ctattgcagc agaagcgaac agccgtcttg ccaggcaaca aatggaacaa
     9721 atgctcagtc tgcaggagaa aattattagt gataccggta gtccatcaac cgtttctgct
     9781 tttctgaata gttaccgaaa aattcaacag gaaagatatg aaaacggaga gatcaaactc
     9841 aacacactga aacagaaagc atcccctctc agagtatttg atgaacgttt tggtaccaga
     9901 ccattagatg ccataaccgt aaaagatgtg gtatcggtgc tggaagatta caaggcaaaa
     9961 ggacataaca gaatgggaca aattttcagg aaagtactga tcgatgtttt ccgggaggcc
    10021 cagcaaacgg gcgatgtccc gctaggcttt aaccctgcag aatcggcaaa aaaaccacag
    10081 gtacggatat cacggcaacg actgaccttt gatgagtgga tgatgattta taatgcagcg
    10141 gaaaaggatg gttacttttt acagcgtggt atgctgctgg cactgatgac aggccagcgc
    10201 ctttcagata tttgcaaaat gaaattttcg gatatcaggg atggttatct tcatgtcgaa
    10261 cagcaaaaaa caggaacccg gattgccatc cctctggctc tgcgttacga taaattaaat
    10321 ctcaccctgg atgatgtggt gtcatcctgt cgcgattgcg ttcttagtcc gtggctattg
    10381 caccaccatc atgctaaagg gacagctaag cgcggcggga tggttaagcc agcaacgtta
    10441 accgttgcat ttaaaaaagc ccgggattct gtggattaca actggcgtgc taatggcacc
    10501 ccaccctctt tccatgagca gagatcttta tcagaacgat tgttcagaga acagggaatt
    10561 gataccaaaa ttttgctggg ccattcgaat caaaaaatga ccgatattta caacgacgca
    10621 cgcggtaagg aatggaaaaa actggtcatt tgatgaccag ttttgcagaa gagttttgca
    10681 gaggttttgc agagcaaatt aaaaacggta ccctgcagag aacataaaca cccacggatc
    10741 aaggcgtaca ttgtcgctat aactgccttt agccagttta cgaccatcag atttatactt
    10801 agccatggta tcgatatcca tgtaccagac tgacatgtta accaaccagt cacggttaat
    10861 cagataatca acccccacct gcccggcagc tccccaggaa tctttcagac tgagatcgga
    10921 aagccctgcc tctttgccat gatcgttaaa tccattatca aagaaggtgg tgtagttaat
    10981 accggcccca acgtaaggac ggaatttgct gctggcatca ccaaaatacc actgcgccat
    11041 cagtgttggc ggcagatgat gaacggttgc aatatcgccg gtcgcccggg tgccgatttt
    11101 atggcggaac ggcgtcgctg ccagtaattc cacaccaatg ttgtcggtcg ccatataagt
    11161 aaacgtcagg cccagttgag tgttattggt cacgctgaat ccacccagac ttcctaacgt
    11221 accaccagca ccttctgttg gacgtacggt tgcagaacct gcacgcataa aaaattcgcc
    11281 tgcttcatgc gcaaaggcac tgccagagag aagagttgtt actgccaaag ccgccactgt
    11341 taactttttc atatccgctc cgtcgttatg gttataaaaa cgtgacgaat atacctacat
    11401 tggagtaaca aatgatccta catagatcac attacatatg ataattcacc atttattgat
    11461 ctggattaat ttagaagttg cattgagaat cctggttaac gtgatttaaa tcaattttta
    11521 aatattttcg ctacgcaaat attaactcgt tcgccacttg ccgcggtttg cccacgcctg
    11581 aaggccagca attacctccg ctttacaaag atatgctttt ccatacaaga cctgatgctg
    11641 ccacccgagg cttaaccagg gtacaattgc ccgctaatta acacctgcat aaaactcaag
    11701 gagagtgcat gtctatcacg gcgcagtccg tataccgtga caccggaaat ttcttccgta
    11761 atcaatttat gaccattctg ttggtatcgt tgctatgtgc gtttatcaca gtggtgttag
    11821 ggcatgtttt ctcacccagt gatgcacagc ttgcgcagct caatgacggc gtgcccgtta
    11881 gcggcagtag tgggttgttc gacctggttc agaatatgtc accggaacag caacaaattt
    11941 tgctgcaggc ttcagcggcg tccacttttt caggattaat cggtaacgcc attctcgccg
    12001 gaggcgtaat attaattatc cagctggtgt ctgcgggtca gagagtcagt gcgctgcgag
    12061 ctattggtgc cagtgcgccg atattgccca agttatttat tctgattttt ctgactaccc
    12121 ttttagtaca gattggcatc atgctggtgg tcgttccggg aattatcatg gccattttac
    12181 tggccctggc accggtgatg ctggtacagg acaaaatggg catttttgcc tcgatgcgta
    12241 gcagtatgcg gctgacttgg gcgaatatgc gtctggtggc acccgcagta ctgagctggt
    12301 tgctggcaaa aacactgttg ctgctttttg cctcttcttt tgccgcatta accccggaaa
    12361 ttggtgccgt actggcgaac accttgagca acctgatttc tgccatattg ctcatctatc
    12421 tgttccgcct gtatatgttg attcgccaat aaccttaagc atttgattac ggaatcgtaa
    12481 aatgaagcag tttcttgatt ttttaccgct ggttgtcttt ttcgcgtttt acaagattta
    12541 tgacatctat gcggctactg cggcgctgat cgtcgccacg gcgattgtgc ttatctatag
    12601 ctgggttcgc tttcgtaagg ttgagaagat ggccctgatc acttttgttc tggtggtcgt
    12661 cttcggtggc ttgacgctgt tcttccacaa tgatgagttt ataaaatgga aggttactgt
    12721 catttatgcc ctgtttgcgg gtgccctgtt agtcagccaa tgggtgatga aaaagccgct
    12781 aattcagcgg atgctgggta aagaactcac gctgccgcaa ccggtatggt cgaagctgaa
    12841 tctggcctgg gctgttttct ttatcctttg cggtctggca aacatctaca tcgcattctg
    12901 gctgccgcaa aatatttggg tcaactttaa agtctttggc ctgaccgccc ttaccttaat
    12961 cttcacattg ttaagcggta tctatatcta ccgccacatg ccgcaggaag ataaatccta
    13021 accagactgc cagtccttcg gggctggcgc tgtatctcct ctgttctcct caggaaaatc
    13081 atagtagcat cgcgcctgtg attttccttt taagtcggtt ttaccatgtc tacaacacat
    13141 aacgtccctc agggcgatct tgttttacgt actttagcca tgcccgccga taccaatgcc
    13201 aatggtgaca tctttggtgg ttggttaatg tcacaaatgg atattggcgg cgctattctg
    13261 gcaaaagaaa ttgcccacgg tcgcgtagtg actgtgcggg ttgaaggaat gactttctta
    13321 cggccggttg cggtcggcga tgtggtgtgc tgctatgcac gctgtgtcca gaaagggacg
    13381 acatcggtca gcattaatat tgaagtgtgg gtgaaaaaag tagcgtctga accaatcggg
    13441 caacgctata aagcgacaga agcattattt aagtatgtcg cggttgatcc tgaaggaaaa
    13501 cctcgcgcct tacctgttga gtaatagtca aaagcctccg gtcggaggct tttgactttc
    13561 tgcttactga atttcggtgg tgccgttaat tttaaacagg atattcacca caatcccact
    13621 gcctggctta cccggctcat aacgccatct gcgcatcgca tttttcacct cacgctcaaa
    13681 catgttcgca ggcttggctg agaggatttg tacgttatcc acgcgaccat ccggcgtgac
    13741 gtcaaattta actttaacct gcccttcaat gcgcaatgcc tgtgctcgtg ccggatactg
    13801 cggctgatta cggcttaatg cgcgtggtcc tgaagccaca ctggtaaccg gcttgctggt
    13861 tgcagccgtt gccgtacttg atgtggggcg cgcgggtgcc gtattttcaa acggtgatgc
    13921 cggacgcgac tctacgggtt tgatatcgcg ttttggctgc tcctgtacct ttttcaccgg
    13981 cttcggtttt ggcttaggtt tcggcttcgg cttttcaatg accaccggtg cttcttttgg
    14041 cggttcgggg atcggctcag gttccggttc tggctctacc accggctccg gtggcggctg
    14101 aacggcttgt ggcggttcga gatcagcagg cgcaaccatc gtgacagaaa ttggctgcgc
    14161 aggcgcaggt agttcaataa cctgatgtac cgaggtatag agcagacccg ccacaacagc
    14221 accatgaatg cagaccgaaa gtaacgtcgg ccaggggaag cggcgaggta aatcaagggt
    14281 cattgaagtc ataatcattt cagtagaaaa accaggtctc gattttaaat gcaaatagca
    14341 atcatattca ataaggcaag acgatcagga tcagtgaaaa tgtcattttg ggcgtaattg
    14401 accttacttt atgcatggtc ttaacaaaga catcatcttt ccgttgcaga tcttcgccct
    14461 ttcaaataac gtactttaca actttcccga acaaggagtt gtgcccgtgt tgtatgttat
    14521 ttacgctcaa gacaaagctg atagcttcga aaaacgcctt tccgttcgtc cggcacattt
    14581 agcacgttta cagttactgc atgatgaagg tcgtttgttg acagctggtc caatgccagc
    14641 ggtagacagt aacgatccag gggctgcggg atttactggc tcaaccgtta ttgctgaatt
    14701 tgaatcgctg gaagcagctc aggcctgggc cgatgctgac ccgtacgtag cggcaggagt
    14761 ctatgaacac gtttcagtga aaccatttaa gaaagtgttc tgaatagaga ttcaatattt
    14821 aagggcacgg tttttgcaac cgtgttcttg tttggccttt ccttatagcc ggtcagtgca
    14881 ttcaccacca caagcagata ataactttat ctgtatttct ttatatgatg tgaacatatt
    14941 caggaaaatg ccctttcctg acgcagagtt gctcgctcaa aacctttgat tactgccgat
    15001 aaagtcatct actattgttt aacacattca cgcgctgcat gtatgttcag aaaatgagca
    15061 cgctaagata attaaacaaa attgtggtta cagaggtatc agtgagtcac tgggctacat
    15121 tcaaacaaac agcaacaaat ttatgggtga ccctacggca cgacattctc gcgctggccg
    15181 tctttttaaa tggattgctt atttttaaaa caatctatgg tatgtcggtc aatttgcttg
    15241 atattttcca tatcaaagca ttttcagagt tggatctctc cttgctggca aacgccccac
    15301 tatttatgct cggcgtcttt cttgtcctga actccattgg cttactgttc cgggcaaagc
    15361 tcgcatgggc aatcagtatc attttgttgt tgatagcgct aatttacacc ctgcattttt
    15421 atccctggct gaaatttagt attggatttt gcatttttac gctggtgttt ttgctgatac
    15481 tgcgcaaaga cttctcccac agtagcgccg cagccgggac aatttttgca tttattagtt
    15541 tcacgacatt actgttttac tcaacctacg gtgcgcttta tttaagcgaa ggttttaatc
    15601 cgcgaataga aagtttgatg accgcgttct atttttcgat agaaaccatg tcaaccgtcg
    15661 gctatggcga tattgtccct gtttctgaat cagcacgatt gttcactatt tcggtcatta
    15721 tttccggcat taccgttttt gccacatcta tgacctcaat ttttggtccg cttatccgcg
    15781 ggggattcaa caaacttgta aaaggaaaca atcatacaat gcatcgtaaa gatcatttta
    15841 ttgtttgcgg acattcgatt ctcgccatca atacgatcct gcaactgaat caacgcggac
    15901 aaaacgtaac ggttatcagc aacttgcctg aagatgatat caagcaactt gagcaacgct
    15961 taggcgataa cgctgatgtt atccccggtg acagtaatga cagttcagta ttaaagaaag
    16021 cgggaatcga tcgttgccgg gccattctgg cgctgagtga taacgatgca gataacgcgt
    16081 ttgttgtact ctcggcaaaa gatatgagca gtgatgtcaa aacagttctc gccgtcagtg
    16141 atagcaaaaa cctgaataag attaagatgg tacatccgga tatcattctc tcaccgcaac
    16201 tgtttggcag cgaaattctg gcgcgagttt taaatggtga agagattaat aatgatatgc
    16261 tcgtttcaat gttgttgaac tccggtcatg gcattttcag cgataacgat gaacaagaaa
    16321 cgaaagctga cagtaaagaa tcagtgcaaa aatagagttt aaagacgaaa gagtaaactt
    16381 attgggcacc gcaatggtgc ccagtatatt tagatattgt agatcgcaaa caataacgag
    16441 ttacgctgtt tgtttagaat gcacttcctg atgctgtgga tacgcatatt tcggcgcgat
    16501 tgcccctcaa gccaacgcga tttacgtcgg ctagcctgac gctgcatgcg ccagcgcccc
    16561 acttccgttc tactccgctt catgttaact act
//
LOCUS       DAZGVQ010000114        16534 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000114 DAZGVQ010000000
VERSION     DAZGVQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16534
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.115"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(85..237)
                     /locus_tag="ACHLAG_003585"
     CDS             complement(85..237)
                     /locus_tag="ACHLAG_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0656742.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(502..1236)
                     /gene="cysH"
                     /locus_tag="ACHLAG_003586"
     CDS             complement(502..1236)
                     /gene="cysH"
                     /locus_tag="ACHLAG_003586"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP0656743.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1310..3022)
                     /gene="cysI"
                     /locus_tag="ACHLAG_003587"
     CDS             complement(1310..3022)
                     /gene="cysI"
                     /locus_tag="ACHLAG_003587"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFP0656744.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDHEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3022..4821)
                     /gene="cysJ"
                     /locus_tag="ACHLAG_003588"
     CDS             complement(3022..4821)
                     /gene="cysJ"
                     /locus_tag="ACHLAG_003588"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFP0656745.1"
                     /translation="MTTQVPPSALLPLNPEQLVRLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5140..5502
                     /gene="queD"
                     /locus_tag="ACHLAG_003589"
     CDS             5140..5502
                     /gene="queD"
                     /locus_tag="ACHLAG_003589"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFP0656746.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5580..6851
                     /gene="ygcN"
                     /locus_tag="ACHLAG_003590"
     CDS             5580..6851
                     /gene="ygcN"
                     /locus_tag="ACHLAG_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP0656747.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERCITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHELVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRTWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            6842..7102
                     /gene="ygcO"
                     /locus_tag="ACHLAG_003591"
     CDS             6842..7102
                     /gene="ygcO"
                     /locus_tag="ACHLAG_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP0656748.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQTAERLISACPAGLFSLTPE
                     GDLRIDYRSCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7119..7694
                     /gene="ygcP"
                     /locus_tag="ACHLAG_003592"
     CDS             7119..7694
                     /gene="ygcP"
                     /locus_tag="ACHLAG_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFP0656749.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7842..8702)
                     /gene="ygcQ"
                     /locus_tag="ACHLAG_003593"
     CDS             complement(7842..8702)
                     /gene="ygcQ"
                     /locus_tag="ACHLAG_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFP0656750.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8699..9478)
                     /gene="ygcR"
                     /locus_tag="ACHLAG_003594"
     CDS             complement(8699..9478)
                     /gene="ygcR"
                     /locus_tag="ACHLAG_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFP0656751.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWRDYLRQRMQP"
     gene            complement(9456..10865)
                     /gene="ygcS"
                     /locus_tag="ACHLAG_003595"
     CDS             complement(9456..10865)
                     /gene="ygcS"
                     /locus_tag="ACHLAG_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0656752.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10887..12341)
                     /gene="ygcU"
                     /locus_tag="ACHLAG_003596"
     CDS             complement(10887..12341)
                     /gene="ygcU"
                     /locus_tag="ACHLAG_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFP0656753.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12411..13196)
                     /gene="ygcW"
                     /locus_tag="ACHLAG_003597"
     CDS             complement(12411..13196)
                     /gene="ygcW"
                     /locus_tag="ACHLAG_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0656754.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13515..14792
                     /gene="yqcE"
                     /locus_tag="ACHLAG_003598"
     CDS             13515..14792
                     /gene="yqcE"
                     /locus_tag="ACHLAG_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFP0656755.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14819..16297
                     /gene="ygcE"
                     /locus_tag="ACHLAG_003599"
     CDS             14819..16297
                     /gene="ygcE"
                     /locus_tag="ACHLAG_003599"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFP0656756.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPYAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGLITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYITLMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT         3936 a         4350 c         4172 g         4076 t
ORIGIN      
        1 cacataaaca atataaatta aaaaagggtg caggagacca aaccatcaca attcagtacg
       61 cggggtttta cccccgcgtg gtggctactt cttcgattcg taagcgagaa ctgccttaaa
      121 ctccatacca cgttctctga tactcaactc acacaacgag tcgcctacca tcagcgtgaa
      181 tcccagtact gtaaaacaca gcactatgat tgccacaagg gcatattttg tcagcatgtc
      241 ttgcctccct gcaaagaaga gactaacatt cgaattgttt gggttcgaac gtaagcctca
      301 ggttgataga aatatcgcct ggggcttttg tccatctgga acctcgcgaa tgcttaacgc
      361 cagacagcct caagcacccg acgccattct atacctgata attcttccag cggcgttcac
      421 gtttaaaatc aggaatactg ctcgcaaaat aaaaacgccc ccatcatttc tgacagaggc
      481 gtttaattta tccggcaata tttacccttc gtgcaatcca cattcccttt ttaagccaaa
      541 gaaacgtgtt tcttcttccg acatgccagg ttcccattta cgggttgtat gggtatcacc
      601 gaccgataaa tatccttcat cccataatgg gtgatatttc aggccatgtt tttgcaggta
      661 ctgataaata gttcggttat cccagtcgat aatcggcagc actttaaata cgccacgctg
      721 aattgccagc accggtaaat tggcgcgact gccggattgt tcacggcgca ggccagcaaa
      781 ccaggtttgc gcgttaagtt ctttcagagc ccggttcatc ggttcgactt tgttgatgtc
      841 attgtacttt tcaatgcctt caacgccctg ttcccacagt ttgccgtagc gtgcttcctg
      901 ccaggcggcg ctttcggtag cgcggtacac tttcaggttg agcttgagtt tgtccgttaa
      961 ctcgtcaata aagcggtagg tttccgggaa cagatagccg gtgtcggtga ggatcaccgg
     1021 aatatccggg tgaatctgat tcaccagatg caggctcacc gccgcctgaa tgccaaagct
     1081 ggaagaaagt acatattcac cgggcagatt atccagcgcc caggcaacgc ggccttcagc
     1141 gtccagtttt tccagttcgg cgttagtttc tgccagcgcc agaatgcgat ctaccttcgg
     1201 cagttcgttc agggcgttta gatcgagttt ggacatagat tcctcactgt ttgccttgcc
     1261 tgatgcgacg ctcacgcgtc ttatcaggcc tacaagaccg ggctgatggt taatcccaca
     1321 aatcgcgcgc cggatcgagc accgggcgaa tgatgcccgc acgcaccgta aagtcgccga
     1381 agccttcacc cgcttcgcgc tctttcgccc agcgccctat cagttcatca agcgacgcca
     1441 ggatttccgg ctcggtgatg ttttctttat acatccgtgg gatacgtgtc ccaatgcggt
     1501 tgccgccaag atgcaggttg tagcgacccg gcgctttacc cactaggccc acttccgcca
     1561 gcatcgcgcg accacaaccg ttcgggcagc ctgttacacg catcacgata tgctcatcgc
     1621 tgacaccatg tttcgccatt aaattatcga tgttgtcgat aaaagacggc aggaaacgct
     1681 ctgcttccgc catcgccagc gggcaagtcg ggaacgagac gcaggccatc gagttttcac
     1741 gctgcggcgt gacggcattc attaacccgc tctctttggc gatcttctcg atcttcgctt
     1801 tctcgctttc cggtacaccg gcgatgatca gattctggtt cgccgtaatg cggaaatcgc
     1861 ctttgtggat cttcgcgatc tccagcaggc cggttttcag cggacgcccc ggataatcaa
     1921 ggatgcgacc attttcgata aacagcgtca ggtgccagtt atcatcaatg cccttaaccc
     1981 agccaatacg atcgcctcgt ccggtgaact catatggacg gatcggttca aatttgatcc
     2041 ccgcgcgacg ctccacttcc gctttaaacg tctcaacccc cacgcgctcc agcgtgtatt
     2101 tggttttggc atttttacga tcggttcggt taccccagtc acgctgagtt gtcacgacgg
     2161 cttcggccac cgccagcgta tgctccagcg gcagatagcc aaactcgctc gccgtgcggg
     2221 cgtaggtttt cttgttgccg tgttcgatag aaagcccacc gcccaccagc aggttgaagc
     2281 ctaccagctt gccgttttcg gcgattgcca cgaagttcat gtcgttggca tgcagatcga
     2341 tatcgttctg cggcgggatc actaccgtgg ttttgaactt acgcggcagg taggtctgac
     2401 caaggatcgg ttcttcatca gtagtggcga ctttttcgtg gtcgagccag atctccgcat
     2461 acgcgcgggt acgaggcaac agatgctcag aaatcttctt cgcccactcg tacgcttccg
     2521 cgtgcagctg cgactcgtaa gggttcgagg tgcagagtac gttacggttc atgtcgttag
     2581 ctgtcgccag cgcatcaaga ccgaccgagt gcagcatctg gtgcaccggt ttgacgttct
     2641 ttttcagaat gccgtggaac tgaaacgtct ggcggttggt caggcgaatg ctgccataga
     2701 tggtgttttc accggcaaat ttgtcgatcg cctgccactg tttagtggta atcaccccac
     2761 ccggcagacg acagcgaagc agcatcgcgt ggcgcggctc cagcttctgt tcagcacgtt
     2821 cggcgcggat gtcgcggtca tcctgctgat acatgccgtg gaagcggatc agcaggaagt
     2881 tgtcgccctt aaagccgccg gtcagaccgt cgtttaaatc ttccgcaatg gtgccgcgca
     2941 ggtagttgct ttcaagcttc atgcgctcgg cgtctgtcag ttttccttcg accactaaag
     3001 gccctggatg tttttcgctc attagtagac atctcgctga taacggcgct ctacgcgcag
     3061 ctcacttaaa aattcatccg ccgcttcggt gtccatgcca ccaaattcgg caatcacttc
     3121 cagaagtgcc tgctcaacgt ctttcgccat gcgattagcg tcgccgcaga cataaatgtg
     3181 ggcaccatca ttgatccagc gccacagctc cgcgccctgt tcgcgcagtt tgtcttgtac
     3241 gtaaactttt tctttttggt cgcgcgacca ggcaagatcg atacgtgtca gcacgccatc
     3301 tttgacgtag cgctgccact ccacctgata caggaagtct tccgtaaagt gcggattacc
     3361 aaagaacagc cagtttttac ctggcgcttc gtcggcggcg cgttgctgca taaaggcgcg
     3421 gaacggcgca ataccggtgc ctgggccaat cataatcacc ggggtttctg gattggctgg
     3481 taggcggaag ttatcgttat gttcgataaa tacgcggact tcgccctctt cttccacgcg
     3541 gtcagcgagg aagctggagg caccaccggc acgggcgcgg ccttccacgt cgtaacgcac
     3601 cacaccaacg gtgacgtgta cttcgttctc gacttccgcc tgcgaggagg caatggaata
     3661 gagacgcggc gtcagcgggc gcagcagatt aattagcgct tcggcatcaa gctgtgccgg
     3721 ggagaaacgc accatgtcga caatcggcgt cgtcgcggcg taatgctgta acttcgcttt
     3781 atcgcccacc agcggcagca gtgtttcgct gcgggtaagc gtggcgtaat tctcaacaat
     3841 gttggcggtg ttgacggtca gttcgaagtg ccactgtagc gcttcgttca gaggcaacgt
     3901 tttgccctcg acggtgacag gttcatcgcc tttcagccac agcagttcga caagttcttt
     3961 caccagtgcc ggatcgttct gataccagac gcccagcgcg tcacccggct ggtaacgcag
     4021 gcccgagtca cctaagtcaa tttcgatatg gcgaacgtct ttttcagagt tacgcccggt
     4081 aattttctgg ttaacagaga ggctagccac cagcggcgcg tctttgctgt acgggctggt
     4141 gtggatttca tttaccgcgc cagtagcgac ggattgcgaa ggtgccgcga caggcgcacg
     4201 cgatttaagc gcatcaacca cgcgggcgcg ccactcgctg gcagcagcct ggtattcaac
     4261 atcggcatcg acacggtcga gcaggcgttc accacccagt tccgccagct tgctgtcaaa
     4321 atctttcccg gactggcaga aaaattcata agagctatcg ccgaggctaa acacggcaaa
     4381 tgcggtgttt tccagcttcg gcgctttttt ggagaacagg aacttatgca gcgcgacggc
     4441 ttcttccggc ggttcccctt ctccttgcgt tgacgtcacc acgataagca gtttttcgct
     4501 ggcgatttgt ttgaatttat agtcgcccgc gttcaccagc ttaacgttca gttttgccgc
     4561 taataaatca tcacgtaatg cttcagcaac acggcgcgca ttgccggttt gcgaggcgga
     4621 gataatagtt atacccggca tttctgcggc gggagctggc gtcgctgcaa gcgcagcagg
     4681 ctgctgattg agcacgcccc agaaatagcc agaaacccag gcaagctggg tgggagttaa
     4741 atcggtcgtg gccgcctgaa ggcgtaccag ttgctccggg ttcaacggaa gcaacgcgga
     4801 aggtgggacc tgtgtcgtca tgcgtcgtta tgttccagta agcaaagctg tttctgcgcc
     4861 ctgtcagcgc ccataaaaca gaagagaagg taaggttaac ggggtgaacg gtgtggatta
     4921 aagacgggat agcgataact aataaccaaa tcgactaacc tgttttagca atagtcttta
     4981 acaacaaaat agattaacca acctaatgaa aaacaaatga atttagccaa tcattaagat
     5041 aaatcagcga ttttgcgcaa caaagtcgtt ttagataatg cgaaaaaaca gcctttccgg
     5101 tactctacgg cggttttatc gtcctgtaga gaaattatga tgtccaccac gttatttaaa
     5161 gatttcacct tcgaagccgc tcaccgctta ccacacgtcc cggaagggca taaatgtggt
     5221 cgcctgcacg ggcattcctt tatggtgcga ctggaaatta ccggggaagt cgatccgcat
     5281 acgggctgga ttatcgattt cgctgaactg aaagcagcgt ttaagccaac ttatgagcgc
     5341 ctcgatcacc attatctcaa tgatattcca ggcctggaaa acccgaccag cgaagtttta
     5401 gcaaaatgga tttgggatca ggttaaaccc gttgtgccgc tgttaagtgc ggtgatggta
     5461 aaagaaacct gcaccgctgg ttgtatctat cgcggcgaat gataagagtg tgtcggcggt
     5521 caatttccct taagtaacgc tatgttaggg tgttgtgttc tggatatctg gggcatgaca
     5581 tggaagacga ctgcgacatt attattattg gtgccggtat tgcaggcacc gcttgcgcgt
     5641 tacgctgcgc gcgagcgggt ttatccgttt tgttactgga acgcgctgaa atccccggca
     5701 gcaaaaatct ttccggcggg cggttatata cccatgcact cgcggaactc ctccctcagt
     5761 ttcatctgac cgcgcctctt gaacgatgca tcactcacga aagcctttcc ctgttaacgc
     5821 cggatggcgc aacgacgttt tccagcttac agcccggcgg tgaatcctgg agtgtattac
     5881 gtgcacggtt cgatccgtgg ctggttgccg aagccgaaaa agaaggtgtc gaatgcatcc
     5941 ctggtgcgac ggtggatgca ctgtatgaag aaaacggcag ggtgtgtggt gtcatttgtg
     6001 gtgacgatat tctccgcgcc cgttatgtgg tgctggcaga aggtgccaac agcgtcctgg
     6061 ctgagcgtca cgagttagtg actcgtcctg ctggcgaagc gatggcgttg gggatcaaag
     6121 aagtgctgtc gctggaaaca tccgctattg aagaacgttt tcatctggag aataacgaag
     6181 gcgcagcgtt gctgttcagc ggcgggatct gtgatgactt acccggcggc gcatttcttt
     6241 atactaatca acaaacgctc tcgttaggga ttgtttgccc gctctcttcc cttacgcaaa
     6301 gccgtgttcc ggcaagcgag ctgctgactc gctttaaagc gcatccggca gtgcgcccgc
     6361 ttatcaaaaa cacggaatca ctggagtatg gtgcgcatct ggtgccagaa ggtggcttgc
     6421 acagtatgcc ggtgcaatac gccggtaacg gctggctgct ggtgggcgat gcgttgcgca
     6481 gttgcgtcaa taccggaatt tccgtgcgcg gcatggatat ggcgctgact ggcgcgcagg
     6541 cggcggcaca aacgctgata agcgcctgcc agcaccgcga gccgcaaaat ctgtttccgc
     6601 tttatcatca caacgtagag cgcagcctgc tgtgggatgt tctacagcgt tatcagcatg
     6661 ttccggcgct tttgcaacgc ccgggatggt accgtacgtg gcctgcgtta atgcaggata
     6721 tttcccgcga tttatgggat cagggtgata aacctgttcc accgctgcgc cagttattct
     6781 ggcatcattt acgtcgtcac ggcctgtggc atctggcggg cgatgttatc aggagtctgc
     6841 gatgtctgta gcccgtaatc tctggcgcgt tgctgatgcg ccgcacattg ttccggctga
     6901 ctccgttgag cgccagacgg cagaacggtt gattagcgcc tgtccggcag gtcttttttc
     6961 gctcacaccg gaaggtgact tacgtattga ctatcgcagt tgcctggagt gtggcacctg
     7021 ccgtttgctg tgcgacgaat caacactaca acagtggcgc tatccgcctt ccggattcgg
     7081 catcacctac cgctttggat aaataataag gataatttat gcccctctta cacctgctcc
     7141 gccagaatcc ggtgattgct gccgttaaag ataatgccag cctgcaactg gcaatcgatt
     7201 ctgaatgcca atttatttcc gtgttgtacg gcaatatctg caccatcagt aatatcgtta
     7261 aaaagattaa gaacgccggg aaatatgctt ttattcatgt tgatttgctg gaaggcgcgt
     7321 caaataaaga agtcgttatt cagtttttga aactggtgac cgaggcggac ggcattatca
     7381 gtaccaaagc ctcaatgttg aaagcggcaa gagcggaagg ttttttctgt attcatcgcc
     7441 tgtttattgt tgattcaatt tcgtttcaca acattgataa gcaagttgcg caatcgaatc
     7501 cggattgtat tgagatcctg ccaggctgta tgcccaaagt gctgggctgg gtgacagaga
     7561 aaatccgcca accgctgatt gccggtgggc tggtgtgcga tgaagaagat gcgcgtaatg
     7621 cgattaacgc gggtgtcgtg gcgctttcca ccacgaatac cggggtctgg acgttagcga
     7681 aaaaattact ttgacgggat aatcgtaacc aattgaattt ggtttgattt gtaggccgca
     7741 cgccacatcc gacattcagc gcctgatgcg acgcttgacg cgtcttatca ggcctacaag
     7801 tccccaggcc agaaccgtag gcaggatcat ccggcaacac gttattggca atctgcgtga
     7861 atgtttgtca ccagtgcctc cagcaccact ttccagtcat caaccacgcc aacatctgcc
     7921 tgtgaaaata ccgcagcgct ggcgtcatga ttaatcgcca ccacaaactt gctattgcgt
     7981 acgcccgcca ttaacgctgc tgcgccagaa gcgccaacaa caatacacac ctcaggtgcc
     8041 agcagatgtc cggaaatacc aatcactttt tcagcatcaa cgccaccgtt catcactcgc
     8101 gcccgactgt agcccacttc ggctcccagc ttttctgcca gcatagcgat ttcctggtta
     8161 tctgcctctc cgccctgccc caccaccagc acccgtctgg cctcagctaa tgggtcgcga
     8221 gtaacatttt tcagatcctc tgtgctaacc agccagtccg gaagcgcacc cgggacaatg
     8281 ttcagttgtt gcattccaga gggcaacgtg gcgtttttcg ctgctcccgc ctgacgcgcc
     8341 agggaaaggc atagcgggcg tttttctgtt tgcagcgtcg ccgttagcgc atttccccag
     8401 tgggatttgc gcacgcttac ggttggaata tctaacgaag tcacctggca gatgcttgca
     8461 ccatgtaaac gccaggccag ccgggtcgac agttcatcgc caaacgttcc cggcggaaag
     8521 agaacaacgt ccgcaggcgt tcgctgccac tgttccacca acgcatccag cacctgttcc
     8581 gccactacag gctgaggttc aatctgccag tgcgccaggg tacaaccgct gaaatcctgc
     8641 gccgccagcc agcttgctat tgcggcattc tcctgattta ttgtcacaat tgcgatattc
     8701 atggctgcat cctttggcgt aaatagtccc gccatagctt ttgagctttt tctgctaccg
     8761 tttgcccatc aatcagcgtc gctccgcgtc gttgctctgc tcttgccagt tgcagacact
     8821 gcatcgctgg catttcggca gcaacagttt tacggataat ttctgctttc cccgcagcca
     8881 ttcgctggcg cattcccggt actggcaagg ccacttcacc gcactgacgc acggcaatca
     8941 ccgcaggcaa gcgaacccgg caacaacgca acccatgttc agtacgctgt tcgagggtga
     9001 taaacagcgc gtcgagagtg aaacgctcca cctgggtgaa gcagggccag cccagcattt
     9061 ccgccagcaa aaatggcgtt tgcccattct gcccttcgct gctttggcag ccggtaatta
     9121 tcagatccag cggattctga tgctgccatt cggcaatatg gcgagcgaca aattccggcg
     9181 caaagcgcag atctgccgcc gtctccagca atacagcttc ctcaaaccca agagccatga
     9241 gatagcgcag ccagtgcaat gcccgttcat cccccatgct caacgcggtt aaagacatcg
     9301 gcgtaccgtt ttttctctgc gccaacagca gcgcggcagc ggcctgttca tcagcaccga
     9361 gtaaacttcg cagtagcgaa atatccggtc cgcttttacc ctgagccgcc gcctgccact
     9421 ctttttccgc cagcattccg gcatccggtt cggctttaaa cgctaacaga atgttcattc
     9481 gcacctccta catttcccgc cgccaccagc gggagggctt tagtttctgg tgcccatagc
     9541 caggtcacaa caaaaccaac caacaacact gtcgccagga gcaataaggt gacttgcatt
     9601 ccccactgcg ccagcaccca cggcagcagg ccagtactta ccgccgcgcc aagtcgactc
     9661 atggcggtgg caaaaccgac gcccagcgag cgaatgtcag taggaaaact ttccgcaggc
     9721 aaaatgccca ccagattact gactgccgaa atggtggtgc tgaagagaac aaaaagcagc
     9781 agcgttaatg aactgccgga aggcaaacag gccattacta ccagcgttgc cgccagcagc
     9841 aaaaaacttc ccagcaaaaa tttgcgatgt gccagcaggt gcgtcagaac taatcccagc
     9901 agcgcgccca caattaacaa cgcattaagc atcaggctgg cagtcagcgc atcttccaga
     9961 ccaatagtct gggcgatagt tggcagccag gtataaatca caaaccatgg gattacgagg
    10021 cagacaaaga agacgctgtt aaacgccgtg cgccgccagt aacgcgaaga gaacaaggtt
    10081 ttgatgtgtt tatgggtcgc cgttaccact tcatcgccca gtaaaacatg gggaccaaaa
    10141 tagcgatgca cgatagcgtg agcttctgca aaacgcccct ggcgcagtag ccagcgtggt
    10201 gattctggcg ttccccagcg taataacgta atcaacaacg cgggcagagc tgccgatgcc
    10261 agtagccagc gccaggcctc cgggttttcg gaaataaagt gatgtccggc aatacttgcc
    10321 agcacatagc ctacggtcca caccacgctg aatgcgccca gcaaaatacc gcgatggcgg
    10381 cgcggggaaa attcagccag caaggtgtga cctactgaat aatcgcctcc cagaccaatg
    10441 ccaatcaaaa tgcgcagtcc aataagatgc tctggcgtgg tggcaaaaaa ttgtaaaaac
    10501 gaagcaagcg taatcagcaa aaagctgaag gtgaagattt tttgccgacc aatatggtcg
    10561 gagatccacc caagaaccag gctgccaagg aacaaaccaa ggagcgccga gccgccgatc
    10621 attcccgcca taaacggcgt cagttgcatg gcgggcgtaa gctgaataat ggcgtaacca
    10681 atgacgccga gaacataacc gtcggtcagg tgtgcgccga aagtgagcgc agcaatgcgg
    10741 cagtgaaaac ggttaagcgg taaatcatcc attcgcaccg gtgaagtgtt catctttacc
    10801 ccttcattgt ccggtaataa gaacggcttc tcgcctgaga agccgaaaag gcaacgcccc
    10861 gtcatcgcgg aacgctgcct gacacattat ttttcaatcg gatagatagt acccgtattc
    10921 ataatgccat taggatcgaa ctgctttttc agcccttcca gcaacgccca cgcgctgccg
    10981 tgttccagtt tgctccagtg aacgcgatgt ttaccgatac cgtggtggtg caccatcgaa
    11041 ccgccgaggc gaatggtttc ttcacagatg atcttgttga gcggattgtg gtacttgtcg
    11101 atttcctctt ccggcttaca gtcaacgacg ttgtaatcgt agacgaagta catgttggtg
    11161 ccgttctgat agctatgaga ggaatgaccg cccagcatgg tgatgtcgtc ggcgtgcggg
    11221 aactcagtac gaatacggtt aataacgctt tcgtagattt cgtggatgca gctccagcag
    11281 ccggacactt cagtggtaaa gcccatgttg ccggttttga ggatctgcac acgttcggca
    11341 gccactttat ccggtcccca gttcaggttg ttaaaccagg tttcgatcag cttgctgtcc
    11401 acgcgctggc attgcgggta gcgggcaacg atttccgcaa tcccttcgcc cgtcgccttc
    11461 gcaatgcgag ggttaccttc agccataaag atcagcacgc attttccgtc tgcaaaatgg
    11521 gtgaagtgtt gggtgccatc ttcagcgtca tacaaacgag cgatcgacgg acgataccct
    11581 tccaccatga cttcacgcag gatgttgaag ccggttttca tgtcttccag gatatagccg
    11641 tagaagaggt tgttttccgg ggtgaattta aagattttca ctgttacttc agtgatatag
    11701 caaagtgcac cttcgttacc gatgatgatg tggcgaatgt ccgggccagc cgcgcggcgt
    11761 ggcacgtttt taatgcgtgt gacggtgcca tcagccaata ctgcttccag accaacgacc
    11821 atatcttcga ttgcgccgta gagtgtggag aactgcccga tactgcgggt tgctaccagg
    11881 ccgcccatct gagccagcgg ctttgactgc ggagaatgcc ccgtggtgta acctttttca
    11941 cgcaacgcat tttccagcac ctccagcgga acgccacatt gcgccgtcgc ctgcatattc
    12001 tcaatatcaa tattaatgat ttgattcatg gcggagccgt cgagcaccac cgagttttcc
    12061 acaacggttt ccagcccacc ttcagtggcg gaagcgccgg tacgcggcac accgttaatt
    12121 ttgtgcgcat tcataaaatt cagcacacgg gatacttgct ctgtggaacc gagttttacg
    12181 accgctgccg gaatcggcaa agtataaatg ccatgaatat ccggaaattt acgaaaacgg
    12241 tcaatactgt tcttctttaa tactgtttca tcggtaatta cgcgatctgc accaacaatt
    12301 tcctttagtt ggtcgacaat cgctgcgcga gataaagaca tagtaattcc ttcctgataa
    12361 taaaaatgga ataatcatta ataagagaaa acagaaacca ccgttatgga ttagcgcact
    12421 aaataaccac catcgaccac taataaatgc ccattgacat aatttgaagc cggacttgcg
    12481 aggaatacag ctgcgcccat taaatcctga gtatcgcccc aacggtttgc cggaatatga
    12541 tcaagaacgc gctgattggt ttctggatta ctgcgtgtcg ccagcgtaat atctgttgca
    12601 taatagccag gggcaatacc atttacctga atattatatt gacctagttc atcacaataa
    12661 gctttggtga atccggcaag agcatgttta gtggcagaat atgcaggtga ccattgtcca
    12721 cctaagtaag agaacaatga acagatatta ataattttgc cgcttttctg cgggatcata
    12781 atttttgcag cttcatagct taactcgaat gcggcggtca ggttcacatc aatcatcgga
    12841 tcccagtcgg cacgaccgaa gtccagcacc ttattcagct tacaaatacc ggcattgttt
    12901 accagaatat caactgtacc gaaacgctca cagcaagcag cgataatctt ctgcggcgcg
    12961 ccttctgcgg tgatatccac ctgcatgaag tccacctcaa caccctgttt ttcaatcatt
    13021 tcctttgttt cgccgttatc tttgacgaaa ctaggaataa agatatttgc gccagctttg
    13081 gccaacgcca tggcaaatgc ctggcctaaa ccgctattcc caccggtaac aattgcggtt
    13141 ttacctttca gtgagaaaaa atccattgag aacgcattga gagattcgat tgacatagtt
    13201 agctccaatt tccgtaaggc aaaaaaaaag aaaggtaact tcccccgcga gagggaagtt
    13261 acctttctca ttatcgctgg taactggaga taatttttag cataacggca taactgctaa
    13321 tgtgatcgta atcacagtgt gatattcgta ttgtcaaata gttttaaaaa gcagaagcgt
    13381 gattactctc acacaacacc tctgctttcc atgttatttc ttattgcaat tactagagac
    13441 ctgagaaaag taatccacct gaaagggcgg cttacttttc tctttttttt gtctattaca
    13501 aatgggaata cgatatgcaa cacaactcat atcgccgttg gataaccctc gcgataatta
    13561 gttttagcgg cggcgttagt tttgacctgg cttatttacg ttatatttat caaattccca
    13621 tggcgaaatt tatgggattc agcaataccg agataggttt aataatgagt acctttggta
    13681 ttgcggctat tattctttat gcccccagcg gcgttattgc cgataaattt tcacaccgca
    13741 aaatgattac ttccgcgatg atcattaccg gattactggg tctgttaatg gcaacgtatc
    13801 caccgctgtg ggtaatgctc tgtattcagg tcgcctttgc gataacgacg attttaatgc
    13861 tgtggtcggt gtcgattaaa gccgcctcgt tgctgggcga tcatagtgag caagggaaaa
    13921 ttatgggctg gatggaaggg ctgcgcggcg tcggtgtaat gtcgctggcg gtgtttacca
    13981 tgtgggtctt ttctcgcttt gcaccggatg acagcgccag cctgaaaaca gtcattatca
    14041 tctacagtgt ggtttacatc ttgttgggga ttctgtgctg gttttttgtt agcgataaca
    14101 acaacctgcg cagtgccaat aacgaagaaa aacagtcatt ccagcttagc gacatccttg
    14161 ccgttttgcg tatcagcacc acctggtatt gcagcatggt gatttttggt gtcttcacca
    14221 tctacgccat tctgagttac tccaccaact atctgaccga aatgtacggc atgtcgctgg
    14281 tggcggcgag ctacatgggg attgtgatca acaaaatctt ccgcgcgctg tgcggcccac
    14341 ttggcggcat tatcaccacc tacagtaaag tgaaatcccc tacccgcgtg atccaaatcc
    14401 tttccatcat cggtttgctg gcgttgacag ccctgctcgt cacgaactct aacccgcaat
    14461 cggtcgcgat ggggattggc ctgattttac tgctgggatt cacctgttac gcctcacgcg
    14521 ggctgtactg ggcctgcccc ggcgaagcga gaacaccgtc ttacattatg ggcaccacgg
    14581 taggtatttg ttcggtgatt ggattcctgc cggatgtctt cgtttacccg attatcggcc
    14641 actggcaaga caccctgccc gctgcagaag cctaccgcaa tatgtggctg atgggcatgg
    14701 cggcgctcgc catggtgatt gtctttacct ttttgctgtt ccaaaaaatt cgtactgctg
    14761 atagcgcccc cgcaatggct agcagcaagt aagccaggaa aattacagga gaaaaatcat
    14821 gtcgaaaaaa tacatcatag ggattgatgg cggaagtcag agcacaaaag tggtgatgta
    14881 cgatctggaa ggtaatgtgg tttgcgaagg taaaggctta ttacagccga tgcacacgcc
    14941 ttatgccgat actgcagaac atcctgacga cgatttatgg gcatcattat gttttgcagg
    15001 tcacgatttg atgagccagt ttgccgggaa caaagaagat attgttggca tcggcctggg
    15061 ctccatccgt tgctgccgtg cgttattgaa agccgacggc acgccagctg cgccgttgat
    15121 tagctggcag gatgcacgcg ttacacgccc ttacgaacat acgaatcctg acgtggcgta
    15181 tgtcacctct ttttcgggtt atctgacgca tcgcttaacc ggcgagttta aagacaatat
    15241 cgccaactat tttggtcagt ggccggtgga ttataagagc tgggcatgga gcgaagatgc
    15301 tgcggtaatg gataagttta atatcccccg tcatatgctg tttgatgtgc aaatgcctgg
    15361 caccgtcctc ggacttatca caccacaagc cgcactggcg acacatttcc cggcaggact
    15421 gccggttgtt tgtaccacca gtgataaacc ggtagaagct ctgggggccg gattactgga
    15481 tgatgaaact gcggtaattt ctcttggcac ttacatcaca ttgatgatga acggcaaagc
    15541 actgccgaaa gatccggtgg cgtactggcc gattatgtct tctattccgc aaacattgct
    15601 gtatgaaggt tacggtattc gcaaaggtat gtggacggtg agctggctgc gtgacatgtt
    15661 aggcgagtcg ttaattcagg atgccagggc gcaggatctt tcaccggaag atttactcaa
    15721 caagaaagcc tctagtgtgc cacctggctg taatgggctg atgacggtgc tggactggct
    15781 gaccaatccg tgggaaccgt acaaacgcgg gattatgatc ggctttgatt ccagcatgga
    15841 ttacgcatgg atatatcgtt cgatattgga aagcgtggcg ctaacgctga agaacaatta
    15901 cgacaatatg tgtaatgaaa tgaatcactt tgcgaagcat gtgatcatta ctggcggcgg
    15961 ttcgaacagc gatctgttta tgcaaatttt tgccgacgtg ttcaaccttc cggcacgccg
    16021 taacgccatt aacggttgtg caagtctggg agcagcgatt aatacagcgg taggtctggg
    16081 gctatacccg gattacgcaa cggctgttga taagatggtt cgcgtgaaag atatctttat
    16141 accgattgag agcaatgcca aacgctacga cgcgatgaat aaaggcattt tcaaagacct
    16201 aaccaaacat actgatgtga tcctgaaaaa atcgtatgaa gtgatgcatg gggaattggg
    16261 gaatgcggat tcgatccaga gctggtcgaa tgcgtgaata aggtaggccg ggcggaaaac
    16321 tgcccggcct gaacatacct gaatggttat ccccgctgac gcggggaaca taagttcgac
    16381 aacttaaaaa atgataacaa gtccggttta tccccgctga tgcagggaac ataataaaaa
    16441 ctttaactag ttcggtttat ccctgttttt acaaggaata ttaactacac tcgatgtctt
    16501 tagaataaaa gacaatacga atcgcacttt atca
//
LOCUS       DAZGVQ010000115        16173 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000115 DAZGVQ010000000
VERSION     DAZGVQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16173
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.116"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            312..1364
                     /gene="yhiM"
                     /locus_tag="ACHLAG_003600"
     CDS             312..1364
                     /gene="yhiM"
                     /locus_tag="ACHLAG_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFP0656757.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
     gene            complement(1680..2882)
                     /gene="yhiN"
                     /locus_tag="ACHLAG_003601"
     CDS             complement(1680..2882)
                     /gene="yhiN"
                     /locus_tag="ACHLAG_003601"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP0656758.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            3114..4613
                     /gene="pitA"
                     /locus_tag="ACHLAG_003602"
     CDS             3114..4613
                     /gene="pitA"
                     /locus_tag="ACHLAG_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFP0656759.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(4684..5019)
                     /gene="uspB"
                     /locus_tag="ACHLAG_003603"
     CDS             complement(4684..5019)
                     /gene="uspB"
                     /locus_tag="ACHLAG_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFP0656760.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            5410..5844
                     /gene="uspA"
                     /locus_tag="ACHLAG_003604"
     CDS             5410..5844
                     /gene="uspA"
                     /locus_tag="ACHLAG_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFP0656761.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            complement(5943..5996)
                     /locus_tag="ACHLAG_003605"
     CDS             complement(5943..5996)
                     /locus_tag="ACHLAG_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656762.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            6162..7631
                     /gene="dtpB"
                     /locus_tag="ACHLAG_003606"
     CDS             6162..7631
                     /gene="dtpB"
                     /locus_tag="ACHLAG_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFP0656763.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(7680..8432)
                     /gene="rsmJ"
                     /locus_tag="ACHLAG_003607"
     CDS             complement(7680..8432)
                     /gene="rsmJ"
                     /locus_tag="ACHLAG_003607"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFP0656764.1"
                     /translation="MKICLIDETGTGDGALSVLAVRWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8440..10482)
                     /gene="prlC"
                     /locus_tag="ACHLAG_003608"
     CDS             complement(8440..10482)
                     /gene="prlC"
                     /locus_tag="ACHLAG_003608"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFP0656765.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFDLYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            10685..11527
                     /gene="rlmJ"
                     /locus_tag="ACHLAG_003609"
     CDS             10685..11527
                     /gene="rlmJ"
                     /locus_tag="ACHLAG_003609"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFP0656766.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            11599..12951
                     /gene="gorA"
                     /locus_tag="ACHLAG_003610"
     CDS             11599..12951
                     /gene="gorA"
                     /locus_tag="ACHLAG_003610"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFP0656767.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADNILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(13005..13088)
                     /gene="dinQ"
                     /locus_tag="ACHLAG_003611"
     CDS             complement(13005..13088)
                     /gene="dinQ"
                     /locus_tag="ACHLAG_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFP0656768.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            13597..13950
                     /gene="arsR"
                     /locus_tag="ACHLAG_003612"
     CDS             13597..13950
                     /gene="arsR"
                     /locus_tag="ACHLAG_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFP0656769.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            14004..15293
                     /gene="arsB"
                     /locus_tag="ACHLAG_003613"
     CDS             14004..15293
                     /gene="arsB"
                     /locus_tag="ACHLAG_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFP0656770.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            15306..15731
                     /gene="arsC"
                     /locus_tag="ACHLAG_003614"
     CDS             15306..15731
                     /gene="arsC"
                     /locus_tag="ACHLAG_003614"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFP0656771.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(15854..16150)
                     /locus_tag="ACHLAG_003615"
     CDS             complement(15854..16150)
                     /locus_tag="ACHLAG_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP0656772.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
BASE COUNT         3695 a         4198 c         4110 g         4170 t
ORIGIN      
        1 atacaaagca ataatactta tatgttcgat gattttaagc accgagacta tccggtcttt
       61 tactaaataa tggaaaacaa tccatccctg gaagataatt gtaaaaaaaa caacagaata
      121 agaattaatc ttaggataaa tatttattta tcatggcctt ttaaccggta aaaaatggca
      181 ctcggcacag taaaccccgc tatagttatg ctatgcattt gtctttaatg atatctcgtc
      241 gttatattaa caggctgaaa ttatcattaa tgcacatttc attattagca gggatacctg
      301 aggagtttaa agtgaacata tatatcgggt ggcttttcaa attaatccct ttgattatgg
      361 gattaatttg catcgcgtta ggtggctttg tgctggaaag ttcagggcaa agcgagtatt
      421 tcgtcgcggg tcatgtgctg atttctctgg cggccatatg cctggcatta ttcactaccg
      481 catttattat catttcgcag ctcacgcgcg gcgttaatac gttttacaat acattgttcc
      541 ccattattgg ctatgcgggg tcaattatca ccatgatatg gggttgggca ctgttagcag
      601 gcaatgatgt gatggcagac gagtttgtcg ccggccatgt tattttcggc gttggtatga
      661 ttgccgcctg tgtatcgacg gtggcagcgt catccggtca ctttctgctc attcccaaaa
      721 atgcagcggg gagcaagagc gatggaacgc cggtacaggc ttattcttca ttaatcggta
      781 attgcctcat tgccgttccc gttttactca ccctgctcgg tttcatttgg tctattacgc
      841 tgttacgtag tgctgacata actccgcatt atgtcgcggg tcacgtattg cttgggttaa
      901 ccgcaatctg tgcctgtcta attggccttg ttgccacaat tgtccatcaa acacgtaata
      961 cgttttcaac taaagaacac tggctgtggt gttattgggt tatttttctc ggctcaatca
     1021 cggtactgca ggggatatac gtcttagtca gttccgatgc aagcgcccga ctggctcccg
     1081 gcattattct tatttgcctc ggaatgatct gttacagcat attctcaaaa gtctggctac
     1141 tggcactggt atggagacgt acctgttcgt tagccaacag aataccgatg attcccgtct
     1201 tcacctgcct gttttgcctt ttcctggcat cgtttcttgc ggaaatggcg cagaccgaca
     1261 tgggatattt tattccttcg cgggttttgg tcggtttagg agcggtatgc tttacgttgt
     1321 tctcaatcgt ttcaatatta gaagcaggtt ctgctaaaaa ataattgcaa cgtaccggat
     1381 aaaaccagcg ttgaccattt gcgtaacgct ggtttttctt aggcatcatg aaataacgca
     1441 gcattaatgc atagtggtta aggataaaaa aacaacaaag ttactgtttt tttcaacctg
     1501 ttcatatttt ataaagatct ggccactggg tgagttttca atcacctttc cttccacctt
     1561 atattaagcc tggagggttt cagttcacgg gctcattaga aaataatcac aagatagcca
     1621 ctatcaaaat tagctcattt ttaatgcggc tctaataatt ttcatcttta ggtaacaggt
     1681 caggacgact tcgctgcaat caaatcctgc gcacaagccc acgcactcga ccacgcccac
     1741 tggaagttat agccccccag ccagccggta acgtccatca cttcgccgat gaagtacagc
     1801 ccaggcactt tgcgcgcttc catcgtccgt gaagagagtt cgttggtgtc cacgccgccg
     1861 agcgtcactt cggcagtgcg atagccttca gtgccgttgg gttgtacgcg ccagtcggtc
     1921 aatgtgctaa tcagtgcctg ttggtcacgc acgttgagct gttttagcga aacatccggg
     1981 atttgcccga gttgctgtaa gcgttcaacc aaccgcttcg gtagatgaac cgccagtgtg
     2041 tttttcaggc tctgattcgg atgtgcgtta cgctgctcat tcaggaaggt ttcgagatcc
     2101 acatccggta gcagattgat actgacaaat tcacccggtt gccagtagct ggaaatctgc
     2161 aacaccgccg gtccagacaa gccgcgatgg gtgaagagta agttctcacg gaaaacgatg
     2221 ccgttttcag cggtaatcac ggaaggcacc gccacgcccg ccagcacctg taactcttcg
     2281 agcaacggtt tatgcagagt gaatggcacc agacccgcgc gggtcggcag cacgttgagg
     2341 ccaaattgtt cggcaatctt ataaccaaac ggcgacgcgc ccagccccgg cattgacagc
     2401 ccaccggtcg cgatgaccag cttttcgcaa ccgacagtca tgccgttcag ttcaagcgtg
     2461 aagcctgttt catccttcgc cacactcagc acttcgctac gcaatctgaa ggttacattg
     2521 cccttctcgc actcatccac cagcatgtcg acaatctgct gcgcggagtc atcgcagaag
     2581 agttgtccta acgttttctc gtgccaggcg atgccgtgtt tattgaccag atcaatgaaa
     2641 tcccactggg taaaacgcgc gagtgcagac ttacaaaaat gcggattctg gctcagataa
     2701 gcgcctggtt cgacataaag gttggtaaag ttgcagcgcc caccgccaga cataaggatt
     2761 ttgcgccctg gttttttacc attatcgatc agcagaaccc ggcgtcctgc ctgacctgcc
     2821 agcgcagaac agaacatacc cgccgcacca gcgcctataa taatggcatc aaacctttcc
     2881 acgttgcgct cctcttagaa aaaacgggcg tgaattgtaa agattcctca gtggtcgcac
     2941 cagcatcaat attactaaaa ggaagtattt gcctgaatta tataagataa ttattttttg
     3001 agtgaaatcc atacaggagg caaatcaaaa aaagtctata tttcactttg cccgcgccgc
     3061 gaaagtcact gataatgcgc cgcgttcatg tcctcaaaat ggcgtaacgt cctatgctac
     3121 atttgtttgc tggcctggat ttgcataccg ggctgttatt attgcttgca ctggcttttg
     3181 tgctgttcta cgaagccatc aatggtttcc atgacacagc caacgccgtg gcaaccgtta
     3241 tctatacccg cgcgatgcgt tctcagctcg ccgtggttat ggcggcggtg ttcaactttt
     3301 tgggtgtttt gctgggtggt ctgagtgttg cctatgccat tgtgcatatg ctgccgacgg
     3361 atctgctgct taatatggga tcgtctcatg gccttgccat ggtgttctct atgttgctgg
     3421 cggcgattat ctggaacctg ggtacctggt actttggttt acctgcatcc agctctcata
     3481 cgctgattgg cgcgatcatc gggattggtt taaccaatgc gttgatgacc gggacgtcag
     3541 tggtggatgc actcaatatc ccgaaagtat taagtatttt cggttctctg atcgtttccc
     3601 ctattgtcgg cctggtgttt gctggcggtc tgattttctt gctgcgtcgc tactggagcg
     3661 gcaccaagaa acgcgcccgt atccacctga ccccagcgga gcgtgaaaag aaagacggca
     3721 agaaaaagcc gccgttctgg acgcgtatcg cgctgatcct ttccgctatc ggcgtggcgt
     3781 tttcgcacgg cgcgaacgat ggtcagaaag gcattggtct ggttatgttg gtattgattg
     3841 gcgtcgcgcc agcaggcttc gtggtgaaca tgaatgccac tggctacgaa atcacccgta
     3901 cccgtgatgc catcaacaac gtcgaagctt actttgagca gcatcctgcg ctgctcaaac
     3961 aggctaccgg tgctgatcag ttagtaccgg ctccggaagc tggcgcaacg caacctgcgg
     4021 agttccactg ccatccgtcg aataccatta acgcgctcaa ccgcctgaaa ggtatgttga
     4081 ccaccgatgt ggaaagctac gacaagctgt cgcttgatca acgtagccag atgcgccgca
     4141 ttatgctgtg cgtttctgac actatcgaca aagtggtgaa gatgcctggc gtgagtgctg
     4201 acgatcagcg cctgttgaag aaactgaagt ctgacatgct tagcaccatc gagtatgcac
     4261 cggtgtggat catcatggcg gtcgcgctgg cgttaggtat cggtacgatg attggctggc
     4321 gccgtgtggc aacgactatc ggtgagaaaa tcggtaagaa aggcatgacc tacgctcagg
     4381 ggatgtctgc ccagatgacg gcggcagtgt ctatcggcct ggcgagttat accgggatgc
     4441 cggtttccac tactcacgta ctctcctctt ctgtcgcggg gacgatggtg gtagatggtg
     4501 gcggcttaca gcgtaaaacc gtgaccagca ttctgatggc ctgggtgttt acccttccgg
     4561 ctgcggtact gctttccggc gggctgtact ggctctcctt gcagttcctg taatcgtaca
     4621 caccaaaacg agcgggtcag tttactggcc cgcttttttt atgcgttatt attgctgaag
     4681 tgcttaatgc caaatcatca atgcaatcag gctgaccacc accagaccac acaatgcgct
     4741 ggtcagaata aactgccgac gcacccgctc acagcggcga ataaattcat catcgtgatg
     4801 atcgcgataa cgttgggcat agatatacca aacgagacgc acctgtttgt tgggttggcc
     4861 atgtgaggta aaaaagcccc ctccatcaac atattgatag agcaatggat cgcagttacg
     4921 cagtaccact aacaacgcgc gtagtgatga gaaatagcgc gccatgttaa caatgcaaac
     4981 gacacataaa gcccaaaata atgcgacggt gcttatcata cctcctcccc ggcgacctgc
     5041 ccgcggagtt ccaccccggg gctaccgctc ccgatacgct gccaatcagt taacaccagg
     5101 tcctggagaa accgcttttg tggtgaccaa catccgagcg gctctataga tagtgtagga
     5161 gatcaggttg ttttttttcc agaaggttaa ccactatcaa tatattcatg tcgaaaattt
     5221 gtttatctaa cgggtaagca aggcggattg acggatcatc cgggtcgcta taaggtaagg
     5281 atggtcttaa cactgaatct ttacggctgg gttagccccg cgcacgtagt tcgcaggacg
     5341 cgggtgacgt aacggcacaa gaaacgctag ctggccagtc atcgacaact ttatggaagg
     5401 agtaacacta tggcttataa acacattctc atcgcagtcg atctctcccc ggaaagcaaa
     5461 gttctggtag agaaagcagt ctctatggct cgcccctaca atgcgaaagt ttctctgatc
     5521 cacgtagatg taaactactc tgacctatac accgggctta ttgatgtgaa tctgggtgat
     5581 atgcagaaac gcatctctga agagacacat catgcgctga ccgagctttc cactaatgca
     5641 ggctacccaa tcactgaaac cctgagcggc agcggcgacc tgggccaggt tctggtcgat
     5701 gcaatcaaga aatacgatat ggatctggtg gtttgtggtc accaccagga cttctggagc
     5761 aaactgatgt cttccgcacg tcagctgatc aacaccgttc acgttgatat gctgattgtt
     5821 ccgctgcgcg acgaagaaga ataatcatcc ctccacgacg tgttcctgaa cgcccgcata
     5881 tgcgggcgtt ttgctttttg gcgcgccttg ttacctgatg cagcgtaaac cccttatctg
     5941 gcctacgatc tgcgtacgca atcaaaatct cctgccaata caacatttaa caccatcata
     6001 ttttccatca ttagtgtgat cagctggtta ttttctgttg taatagtgta ttaatctatt
     6061 caccgcatca atattaagaa tctctgacag atgtaaactt tttcgcgcgt tatcccttac
     6121 gcgttcatac ttttcaggat ggtattggaa ggttaataaa tatgaataca acaacaccca
     6181 tggggatgct gcagcaacct cgcccatttt tcatgatctt ttttgtcgag ttatgggagc
     6241 gattcggcta ctacggcgtg cagggcgtac tggcggtttt cttcgttaaa cagcttggat
     6301 tctcgcaaga gcaggctttt gtcacttttg gtgcttttgc tgcgctggtc tatggcctca
     6361 tttccattgg cggctatgtc ggcgaccacc tgctggggac caaacgcacc attgttctcg
     6421 gagcacttgt gctggcgatt ggctacttca tgaccggcat gtcgctactt aagcctgacc
     6481 tgattttcat cgccctgggg actatcgctg tcggtaacgg cctgtttaaa gctaacccag
     6541 ccagcttgct ttcgaagtgc tatccgccga aagatccgcg gcttgatggc gcattcaccc
     6601 tgttctatat gtcgatcaat atcggctcgt tgatagcgtt atcgctggcc cctgtgatcg
     6661 ctgatagatt cggttattca gtcacctaca acctgtgcgg ggcggggtta attatcgcat
     6721 tactggttta catcgcctgt cgtggaatgg tgaaagacat tggttctgaa cccgacttca
     6781 agccgatgag cttcagcaaa ctgttgtacg tattacttgg cagcgtggtg atgatcttcg
     6841 tatgtgcatg gctgatgcac aacgtagaag tcgccaatct ggtgctgatt gttctctcca
     6901 tcgtcgtcac catcatcttc tttcgtcagg cattcaagct ggataaaacc gggcgcaata
     6961 aaatgtttgt cgcctttgtc ctgatgctcg aagcggtggt gttttacatt ctctacgccc
     7021 agatgccaac atcgctgaac ttctttgcca tcaacaacgt gcatcatgaa attctcggtt
     7081 tttccatcaa cccggtcagc ttccaggcgc ttaacccgtt ctgggtggta ctcgccagcc
     7141 caatactggc aggcatttac acgcatctgg gtaacaaagg caaagacctc tcgatgccga
     7201 tgaaatttac tctcggcatg tttatgtgct ctctgggctt tttgacggct gcagctgcgg
     7261 gaatgtggtt tgcggatgcc caggggctga catcgccatg gtttatcgtg ctggtgtact
     7321 tattccagag cttaggtgaa ctgtttatta gcgcccttgg cctggcgatg attgctgccc
     7381 tggtgccgca gcatttgatg ggctttattc tcgggatgtg gttcctgacg caggctgccg
     7441 cgttcttgct gggcggctat gtggcaacat ttaccgcggt gccggacaac attaccgatc
     7501 cgcttgagac gttgcccgtc tataccaacg tgtttggtaa gattggtctg gtcacactgg
     7561 gcgttgcagt agtgatgctg ttgatggtgc cgtggctgaa acgcatgatt gcgacgccgg
     7621 aaagccatta attattcttg cagaaagcag ggtagcgtta tcgctaccct ggtttacttt
     7681 tacactggcg tacctgcata aatatcaaac ctatgccctt tagtgactac cgcgtttggc
     7741 gtggcgacat tcgccagcgg tggcgcgtag tccgggcgct ttaccaccac gcgtttggtc
     7801 gccagcaagc gtgcaggctc cagtaatcca tcggcatcaa gatccggtcc caccagcgac
     7861 tgaaagacac gcatctcttt tttcactagc gcgcttttct gcttatgcgg gaacatcggg
     7921 tcgagataaa ccacctgcgg gcgcggggta atatcagtca gcgccgtcag gctggaggcg
     7981 tgaattaact gcaaccgctc ctgcaaccag ccgccgattt ccgcatccgc ataaccacgc
     8041 gccaggccgt cgtcgagcaa tgcggcaacg actggattac gctccagcat ccgcacgcgg
     8101 cagccgactg aagccagcac aaaggcatcg cgccccagcc ctgcggtggc atccaccaca
     8161 tccggcaaat aatcgccttt aatgcccacc gctttcgcca ccgcctcacc gcgaccaccg
     8221 ccgaatttgc gtcggtgcgc catcgctccg ccaacaaaat caacaaagat gccgccaagt
     8281 tttggctcat cacgcttgcg caattccaga tgttccggcg ttaacaccag cgccatcagg
     8341 ttgtcttcat cgtgctccag cccccagcgg accgccagaa cagataaggc accgtctccg
     8401 gtgcctgttt catcaattaa gcagattttc actgaatgat cagcccttaa tgccgtaatg
     8461 ctccagcatc gcatccagct gcggttcacg accacggaag cgtttgaaca gatccatcgg
     8521 ctcttctgaa ccgccacggc tcagaatgtt gtcgaggaac gactgcccgg tttcacggtt
     8581 gaaaatgccc tcttcctcaa agcgcgagaa agcatctgcc gccagtacgt cagcccacag
     8641 gtagctgtag taacctgcgg cataaccacc ggcgaaaata tggctgaaag cgtgcgggaa
     8701 acggccccag gatggagacg gtactacggc aaccagtttc ttgatttctg ccagagtttc
     8761 gaggattttc gctccctgat ccgggcggaa ctcggcatgg aggcggaaat cgaacaggcc
     8821 gaactccagc tggcgcagaa taaacagcgc cgcctggtag ttcttcgccg ccagcatttt
     8881 atccagcaac tctttcggca gcggttcgcc ggtttcgtag tgaccggaga taaacgccag
     8941 cgcctccggc tcccagcacc agttttccat aaactgactc ggcagttcga ccgcatccca
     9001 cggcacaccg ctgataccgg aaacaccagc ggtttcgatg cgggtcagca tatggtgcag
     9061 gccgtgaccg aactcgtgga acagggtgat cacttcgtcg tgagtgaaca gcgccggttt
     9121 accatttacc gggcggttga agttacaagt caaatacgcg accggttttt gcagcgaacc
     9181 atcagcttta cgcatctggc ctacgcagtc atccatccac gccccgccgc gcttgttttc
     9241 acgggcatac agatcgaggt agaagctgcc gcgcagttcg ttattttcgt catacagatc
     9301 gaagaaacgt acatccggat gccagacatc aacatcttta cgctctttag cggtgatgcc
     9361 gtaaatacgt ttcaccactt caaacaggcc gttaaccgct ttgttttctg ggaagtacgg
     9421 acgcagctgc tcgtcgctga tgctgtagag gtgctgtttt tgtttttcgc tgtagtacgc
     9481 gatatcccac ggctgcaact catcgacgcc aaattccgct ttggcgaagg cgcgcagttg
     9541 tgccagctct ttttcgcctt gcggacgcgc gcgttttgcc agatcggtta agaaatccag
     9601 cacctgctgc gggttttctg ccattttagt ggcaagggat ttaaaggcgt agttttcaaa
     9661 gcccagcagt tgcgccagtt cgtgacgcag agcgaggatc tcttccatca ccttgctgtt
     9721 atcccattta ccggcgttcg ggccttgatc ggaggcgcgg gtgctgtaag cgcgatacat
     9781 ctcttcacgc aaggcctggt tgtcgcagta ggtcattacc ggcaggtagc ttgggatatc
     9841 cagcgtcagc aggtagcctt ccagctcttt cgcttcggcc tgggcttttg ccgcagccag
     9901 cgcgctttca ggcatccccg ccagctccgc ttcgtcggta acgagtttgg tccagcccat
     9961 cgtcgcatcg aggacgttgt tgctgtactg gttgcccagc tcggaaagac gagtggcaat
    10021 ctcaccgtag cgttgttgtt tctctattgg cagaccgatg ccagagagtt cgaagtcgcg
    10081 cagtgcgtta tcaaccgctt ttttctgagc cgtgttcagc gtggcgtaat gatcgccatc
    10141 gcgcaggtcg cgatacgctt tatacagccc ttcatgttgc cctacccagg tgctgtattc
    10201 cgacagcagc ggcagggttt gttcgtacgc ttcacgcagt tccgggctat ttttcaccga
    10261 gttcaggtgg ctgaccgggg agaagatacg ccccagcaca tcgtccactt ccgccaacgg
    10321 ctggcagaga ttttcccagg tgtacggtgc tccttgcgct actacgcgct ccacattttc
    10381 gcggcagtcg ttcagcgcct tagtcacggc tggaacgaca tgttccggga gaattttaga
    10441 aaacggaggc aattcaaagg gagtcagtaa cggattcgtc attcgcgcag tcctggttaa
    10501 agaggttaag gaagcgctca acaggcgctt tacataatgt gtgtagcatg gggttaagtg
    10561 tagtgaattt caatgagaaa cgttacgctt tcgcggcggc ggcctctttt cggtatactg
    10621 tcctgatacg cttttgtgcg ccccgaatac gggccgattt ttacttaccg gaacaccttt
    10681 acccatgctc agttatcgcc acagctttca cgctggcaac cacgccgacg tccttaaaca
    10741 taccgttcag agcctgatca tcgagtcgct gaaagagaaa gataaaccgt ttctctatct
    10801 cgacacccac gcaggggccg ggcgttatca gttaggcagc gaacatgccg agcgtaccgg
    10861 cgaatatctc gaaggcatcg cccgtatctg gcagcaggac gatttgcccg cagaactgga
    10921 ggcgtacatc aatgtggtaa aacacttcaa ccgtagcggt cagttgcgtt actaccccgg
    10981 ttcgccgttg attgctcgcc agctactgcg tgaacaggac agcctgcaac tgaccgaact
    11041 gcacccgagc gattacccgt tgttgcgttc tgaatttcag aaagatagcc gtgcgcgtgt
    11101 cgaaaaagcc gacggtttcc agcagcttaa ggccaaactg ccgccggttt cccgccgtgg
    11161 tttaatcctt atcgacccgc cgtatgaaat gaaaactgac tatcaagcgg tggtcagcgg
    11221 gatagcagaa ggttacaaac gtttcgccac tggtacttac gcactgtggt atccggtggt
    11281 gctgcgtcag caaattaagc gcatgatcca cgatctggaa gcgaccggta ttcgcaaaat
    11341 tctgcaaatt gaactggcgg tactgccaga cagcgatcgc cgtggcatga ccgcttccgg
    11401 catgattgtg attaacccgc cgtggaaact ggaacagcag atgaataacg tgctgccgtg
    11461 gctgcacagc aaactggttc cggcaggcac cgggcacgcc accgtaagct ggatcgtgcc
    11521 ggagtaattg cagccattgc tggcacctat tacgtctcgc gctacaatcg cggtaatcaa
    11581 cgataaggac actttgtcat gactaaacac tatgattaca tcgccatcgg cggcggcagc
    11641 ggcggtatcg cctccatcaa ccgcgcggct atgtacggcc agaaatgtgc gctgattgaa
    11701 gccaaagagc tgggcggcac ctgcgtaaat gttggctgtg tgccgaaaaa agtgatgtgg
    11761 cacgcggcgc aaatccgtga agcgatccat atgtacggcc cggattatgg ttttgatacc
    11821 actatcaata aattcaactg ggaaacgttg atcgccagcc gtaccgccta tatcgaccgt
    11881 attcatactt cctatgaaaa cgtgctcggt aaaaataacg ttgatgtaat caaaggcttt
    11941 gcccgcttcg ttgatgccaa aacgctggag gtaaacggcg aaaccatcac ggccgataat
    12001 attctgatcg ccacaggcgg tcgtccgagc cacccggata ttccgggcgt ggaatacggt
    12061 attgattctg atggcttctt cgcccttcct gctttgccag agcgcgtggc ggttgttggc
    12121 gcgggttaca tcgccgttga gctggcgggc gtgattaacg gcctcggcgc gaaaacgcat
    12181 ctgtttgtgc gtaaacatgc gccgctgcgc agcttcgacc cgatgatttc cgaaacgctg
    12241 gtcgaagtga tgaacgccga aggcccgcag ctgcacacca acgccatccc gaaagcggta
    12301 gtgaaaaatg ccgatggtag cctgacgctg gagctggaag atggtcgcag tgaaacggtg
    12361 gattgcctga tttgggcgat tggtcgcgag cctgccaatg acaacatcaa cctggaagcc
    12421 gctggcgtta aaactaacga aaaaggctat atcgtcgtcg ataaatatca aaacaccaat
    12481 attgaaggta tttacgcggt gggcgataac acgggtgcag tggagctgac accggtggca
    12541 gttgcagcgg gtcgccgtct ctctgaacgc ctgtttaata acaagccgga tgagcatctg
    12601 gattacagca acattccgac cgtggtcttc agccatccgc cgattggtac tgttggttta
    12661 acggaaccgc aggcgcgcga gcagtatggc gacgatcagg tgaaagtgta taaatcctct
    12721 ttcaccgcga tgtataccgc cgtcaccact caccgccagc cgtgccgcat gaagctggtg
    12781 tgcgttggat cggaagagaa gattgtcggt attcacggca ttggctttgg tatggacgaa
    12841 atgttgcagg gcttcgcggt ggcgctgaag atgggtgcaa ccaaaaaaga cttcgacaat
    12901 accgtcgcca ttcacccaac ggcggcagaa gagttcgtga caatgcgtta aatgttaaag
    12961 ggctaagagt agtgtgctct tagcccttaa ttacgtttcc gctatcagtt cagaagctga
    13021 agcagaaagc ggatcagttc cagcagcgca attaacgccc caagaacgat gattgcttta
    13081 tcaatcaccc gttttctcca tgcgatggag tgagaatgca tccgcttact catccactgc
    13141 ctgtcacggc acatgcccta ttgttagata atggcattct cactccggcc agagcatcag
    13201 ttaacggcac cacccgtact tctgaccagg actttgaaag cgtttatgcg cattaccaga
    13261 gtgaaaatgc ctcagagcta actggataat catacagtac attcaggtta taaaaccagc
    13321 atgtccttgc aatagtttca gtatggtatt agcattgatg cgttagataa tggcattctc
    13381 actccagcca gtgccaccaa ctcagggctg gaaagtaaaa aaccgacgca aagtcggttt
    13441 ttttacgtcc tgattcagac ctcctttcaa atgaatagcc aactcaaaat tcacacctat
    13501 taccttcctc tgcacttaca cattcgttaa gtcatatatg tttttgactt atccgcttcg
    13561 aagagagaca ctacctgcaa caatcaggag cgcaatatgt catttctgtt acccatccaa
    13621 ttgttcaaaa ttcttgctga tgaaacccgt ctgggcatcg ttttactgct cagcgaactg
    13681 ggagagttat gcgtctgcga tctctgcact gctctcgacc agtcgcagcc caagatctcc
    13741 cgccacctgg cattgctgcg tgaaagcggg ctattgctgg accgcaagca aggtaagtgg
    13801 gttcattacc gcttatcacc gcatattcca gcatgggcgg cgaaaattat tgatgaggcc
    13861 tggcgatgtg aacaggaaaa ggttcaggcg attgtccgca acctggctcg acaaaactgt
    13921 tccggggaca gtaagaacat ttgcagttaa aaatttagct aaacacatat gaattttcag
    13981 atgtgtttta tccgggaggc attatgttac tggcaggcgc tatctttgtc ctgaccatcg
    14041 tattggttat ctggcagccg aaaggtttag gcatcggctg gagtgcaacg ctcggcgcag
    14101 tactggcgtt agttacgggc gtggtccatc cgggtgatat tccggtggtg tggaatatcg
    14161 tctggaacgc gacggctgcg tttatcgccg tcattatcat cagcctgctg ctggatgagt
    14221 ccggcttttt tgaatgggcg gcgctgcacg tctcacgctg gggtaatggt cgtggtcgct
    14281 tgctgtttac ctggattgtc ctgctcggtg ctgccgttgc cgccctgttt gccaatgatg
    14341 gcgcggcgct tattttgaca ccgattgtca tcgccatgct gctggcttta gggttcagta
    14401 aaggcactac gctggcgttc gtgatggcgg ccggattcat tgccgatacc gccagcctgc
    14461 cgcttattgt ctccaacctg gtgaatatcg tttccgctga tttctttggc ctcggctttc
    14521 gcgaatacgc ctcggtgatg gtgccggtgg atatcgccgc gattgttgcc acgctggtga
    14581 tgttacatct ctattttcgc aaagatattc cgcagaacta cgatatggcg ctgctgaaat
    14641 ctcccgcaga agcgatcaaa gatcctgcta cgttcaaaac tggctgggtt gttttactgc
    14701 ttctgctggt gggatttttc gtcctggaac cgctcggcat tccggtgagc gccattgcag
    14761 ctgtgggcgc gctgatatta tttgtcgtcg ctaaacgcgg tcatgcgatt aatacgggta
    14821 aagtcctgcg cggtgccccc tggcagattg tcatcttctc gctcggcatg tatctggtgg
    14881 tttatggcct gcgcaatgcc ggattaacgg aatatctttc tggcgtactc aacgtgctgg
    14941 cggataacgg cctgtgggcc gcgacgctcg gcaccggatt cctcaccgcc ttcctctctt
    15001 ctattatgaa caatatgccg acggtactgg ttggcgcgtt gtccattgat ggcagcacgg
    15061 catctggcgt tatcaaagaa gcgatggttt atgccaatgt gattggctgc gatttgggac
    15121 cgaaaattac cccaattggt agcctggcta cgctactctg gctgcacgta ctttcgcaga
    15181 agaatatgac tatcagctgg ggatattact tccgtacagg gattatcatg accctgccgg
    15241 tgctttttgt gacgctggct gcgctggcgc tacgtctctc tttcactttg taatgagata
    15301 ttgatatgag caacattacc atttatcaca acccggcctg cggtacgtcg cgtaatacgc
    15361 tggagatgat ccgcaacagc ggtacagaac cgaccattat ctattatctg gaaacaccgc
    15421 caacgcgcga tgagctggtc aaactcattg ccgatatggg gatttccgta cgcgcgctgt
    15481 tgcgtaaaaa cgtcgagccg tatgaggaac tgggccttgc agaagataaa tttactgacg
    15541 atcggttaat cgactttatg cttcagcacc cgattctgat taatcgcccg attgtggtga
    15601 cgccgctggg aactcgcctg tgccgccctt cagaagtggt gctggaaatt ctgccagatg
    15661 cgcaaaaagg cgcattctcc aaggaagatg gcgagaaagt ggttgatgaa gcgggtaagc
    15721 ggttgaaata aagcggcgat atccactgcc catagttggt aaagtgcgct ttgtttatgt
    15781 cggatgcgac gctaacgcgt cttatccgac ctacaaaacc gtgcatggca tgaaatctgt
    15841 agcgcgtagc gcatcaggca attttgctat ttacgcagca ctcctcgcgt aagaattcaa
    15901 ttatcgcttc cagcattgcg tattgcgaaa cacacatcag cgtccgactc tcacggtttt
    15961 gtttaaccag ccctaccgat accagtgctg aaatatggtg actgagtgta gagccgggta
    16021 tcccaagctg cttttgcaac tcaccgaccg gcaaaccttg ctcccccgct ttgaccaggt
    16081 gtttgaatat gaacaaacgg gtcgggtgac ctagctcttt cagcgcttta gcaacttctt
    16141 ccaactgcat atgacacccc ctttgtctgg ttg
//
LOCUS       DAZGVQ010000116        15823 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000116 DAZGVQ010000000
VERSION     DAZGVQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.117"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..96)
                     /locus_tag="ACHLAG_003616"
     CDS             complement(<1..96)
                     /locus_tag="ACHLAG_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbF"
                     /protein_id="HFP0656773.1"
                     /translation="MNYRKYQRELIMRENKNNPELKIRSTERDYKY"
     gene            complement(50..391)
                     /gene="trbJ"
                     /locus_tag="ACHLAG_003617"
     CDS             complement(50..391)
                     /gene="trbJ"
                     /locus_tag="ACHLAG_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFP0656774.1"
                     /translation="MPPVLWRGWIPFCRCTEGKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIHQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRRTGLNELS
                     EISEGTYNERK"
     gene            complement(321..866)
                     /gene="trbB"
                     /locus_tag="ACHLAG_003618"
     CDS             complement(321..866)
                     /gene="trbB"
                     /locus_tag="ACHLAG_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFP0656775.1"
                     /translation="MSLTKSLLFTLLLSAAAVQASTRDEIERLWNPQGMATQPAQPAA
                     GTSARTAKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYGGKKGAK"
     gene            complement(853..1137)
                     /gene="traQ"
                     /locus_tag="ACHLAG_003619"
     CDS             complement(853..1137)
                     /gene="traQ"
                     /locus_tag="ACHLAG_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFP0656776.1"
                     /translation="MISKRRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSREEREALEARQQAMMEGQQEGGHVSH"
     gene            1218..1532
                     /locus_tag="ACHLAG_003620"
     CDS             1218..1532
                     /locus_tag="ACHLAG_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin ArtA"
                     /protein_id="HFP0656777.1"
                     /translation="MEKRSFKEKLEIIRNIIRESLLGNAAIIALIYAASHSLPVNAFP
                     DYLVISLLSIAAVIVVLWLFSIIYIYFCELFRSHWIAVWFIIWSSVINLIILYGFYDH
                     FI"
     gene            complement(1534..1881)
                     /gene="trbA"
                     /locus_tag="ACHLAG_003621"
     CDS             complement(1534..1881)
                     /gene="trbA"
                     /locus_tag="ACHLAG_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFP0656778.1"
                     /translation="MSEDYLKMFTGVVLLMFVIIAGYFFSERNDRKMFLLSSLVFLVI
                     NIACLYVLTASLWFLCGAIMSQGAALVVSIVAAALPDVTSFDRFRRIFICIVLSSVWS
                     GVMWFFIRGLMTG"
     gene            complement(1897..2640)
                     /gene="traF"
                     /locus_tag="ACHLAG_003622"
     CDS             complement(1897..2640)
                     /gene="traF"
                     /locus_tag="ACHLAG_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HFP0656779.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSARKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(2633..2890)
                     /locus_tag="ACHLAG_003623"
     CDS             complement(2633..2890)
                     /locus_tag="ACHLAG_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HFP0656780.1"
                     /translation="MKVIFTSNRFIDFLIRLLITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTDLQKPDERKSDERNEDE"
     gene            complement(2917..4725)
                     /gene="traN"
                     /locus_tag="ACHLAG_003624"
     CDS             complement(2917..4725)
                     /gene="traN"
                     /locus_tag="ACHLAG_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001756807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HFP0656781.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFAHQIKGQGSSSIQG
                     FKPQESIPGYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEISRSEYTNYTCERDLQVEQYCTRT
                     ARMELQGSTTWETRTLEYEMSQLPAREVNGQYVVSITSPVTGEIVDAHYSWSRTYLQK
                     SVPMTITVLGTPLSWNAKYSADASFTPVQKTLTAGVAFTSSHPVRVGNTKFKRHTAMK
                     LRLVVRVKKASYTPYVVWSESCPFSKELGKLTKTECTEAGGNRTLVKDGQSYSMYQSC
                     WAYRDTYVTQSADKGTCQTYTDNPACTLVSHQCAFYSEEGACLHEYATYSCESKTSGK
                     VMVCGGDVFCLDGECDKAQSGQSNDFAEAVSQLAALAAAGKDVAALNGVDVRAFTGQA
                     KFCKKAAAGYSNCCKDSGWGQDIGLAKCSSDEKALAKAKSNKLTVSVGEFCSKKVLGV
                     CLEKKRSYCQFDSKLAQIVQQQGRNGQLRISFGSAKHPDCRGITVDELQKIQFNRLDF
                     TNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(4722..5360)
                     /gene="trbC"
                     /locus_tag="ACHLAG_003625"
     CDS             complement(4722..5360)
                     /gene="trbC"
                     /locus_tag="ACHLAG_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HFP0656782.1"
                     /translation="MKLSMKSLAALLMMLNGAVMASENVNTPENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RTVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(5369..6361)
                     /gene="traU"
                     /locus_tag="ACHLAG_003626"
     CDS             complement(5369..6361)
                     /gene="traU"
                     /locus_tag="ACHLAG_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HFP0656783.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVTRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(6358..6990)
                     /gene="traW"
                     /locus_tag="ACHLAG_003627"
     CDS             complement(6358..6990)
                     /gene="traW"
                     /locus_tag="ACHLAG_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HFP0656784.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYGSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPILESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(6987..7373)
                     /gene="trbI"
                     /locus_tag="ACHLAG_003628"
     CDS             complement(6987..7373)
                     /gene="trbI"
                     /locus_tag="ACHLAG_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HFP0656785.1"
                     /translation="MSSTQKPADVTAERRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTVAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(7370..9997)
                     /gene="traC"
                     /locus_tag="ACHLAG_003629"
     CDS             complement(7370..9997)
                     /gene="traC"
                     /locus_tag="ACHLAG_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HFP0656786.1"
                     /translation="MNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRLL
                     PYRDYNQESGLFMNDTTMGFMLEAIPINGANESIVEALDHMLRTKLPRGIPLCIHLMS
                     SQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYRV
                     FISYCSPSKKKSRADILEMENLVKIIRASLQGASITTQTVDAQAFIDIVGEMINHNPD
                     SLYPKRRQLDPYSDLNYQCVEDSFDLKVRADYLTLGLRENGRNSTARILNFHLARNPE
                     IAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTSY
                     AKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSFR
                     KNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADNP
                     LTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSGG
                     FAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPNG
                     NLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAESPTIRSRLDEMIV
                     LLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENRM
                     YRTPRNLKKLNVIDEGWRLLDFKNHKVGEFIEKGYRTARRHTGAYITITQNIVDFDSD
                     KASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFLPLQRDMIGKFGAAKDQWFSS
                     FLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSGP
                     EMASLEAWLEEHEKYRSVA"
     gene            complement(10157..10378)
                     /gene="traR"
                     /locus_tag="ACHLAG_003630"
     CDS             complement(10157..10378)
                     /gene="traR"
                     /locus_tag="ACHLAG_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HFP0656787.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQKINAHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(10513..11028)
                     /gene="traV"
                     /locus_tag="ACHLAG_003631"
     CDS             complement(10513..11028)
                     /gene="traV"
                     /locus_tag="ACHLAG_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HFP0656788.1"
                     /translation="MKQISFFIPLLGTLLLSGCAGTSTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(11025..11276)
                     /gene="trbG"
                     /locus_tag="ACHLAG_003632"
     CDS             complement(11025..11276)
                     /gene="trbG"
                     /locus_tag="ACHLAG_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbG"
                     /protein_id="HFP0656789.1"
                     /translation="MNKLVSDGSVKKINYPVLYESGITPPLCEVSAPEPDAGGKRIVA
                     YVYKSSRSTVFENPDIVKTCTVRDLKKDFVNCDEKGEGQ"
     gene            complement(11269..11589)
                     /locus_tag="ACHLAG_003633"
     CDS             complement(11269..11589)
                     /locus_tag="ACHLAG_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001377845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HFP0656790.1"
                     /translation="MNMRNINVITALSVPGKTVSDDFMHAVLSNCATRIVLPAPEKFG
                     SESLPDNFNMAAVGVMKKGMSMSLFSSPGHIVVAGQSGGGKSVPVKELIDRIIQKYKE
                     PFDE"
     gene            complement(11576..12166)
                     /gene="traP"
                     /locus_tag="ACHLAG_003634"
     CDS             complement(11576..12166)
                     /gene="traP"
                     /locus_tag="ACHLAG_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus-stabilizing protein
                     TraP"
                     /protein_id="HFP0656791.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTALWATLALMYVSLAAITGKYPVRPGKMKCIRVVTADEHLK
                     EVYTEDASLPGKIRKCPVYLPDDRTNRNNGDKNEHA"
     gene            complement(12156..13583)
                     /gene="traB"
                     /locus_tag="ACHLAG_003635"
     CDS             complement(12156..13583)
                     /gene="traB"
                     /locus_tag="ACHLAG_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer pilus assembly protein
                     TraB"
                     /protein_id="HFP0656792.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPASPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(13583..14311)
                     /gene="traK"
                     /locus_tag="ACHLAG_003636"
     CDS             complement(13583..14311)
                     /gene="traK"
                     /locus_tag="ACHLAG_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HFP0656793.1"
                     /translation="MRKNNTAIIFGSLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADAVWTGNHLKMVRFAVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(14298..14864)
                     /gene="traE"
                     /locus_tag="ACHLAG_003637"
                     /pseudo
     CDS             complement(14298..14864)
                     /gene="traE"
                     /locus_tag="ACHLAG_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490565.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
     gene            complement(14886..15197)
                     /gene="traL"
                     /locus_tag="ACHLAG_003638"
     CDS             complement(14886..15197)
                     /gene="traL"
                     /locus_tag="ACHLAG_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HFP0656794.1"
                     /translation="MSGDENKLKKYRFPETLTNQSRWFGLPLDELIPAAICIGWGITT
                     SKYLFGIGAAVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWI
                     K"
     gene            complement(15212..15577)
                     /gene="traA"
                     /locus_tag="ACHLAG_003639"
     CDS             complement(15212..15577)
                     /gene="traA"
                     /locus_tag="ACHLAG_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HFP0656795.1"
                     /translation="MDAVLSVQGVSAPVKKKSFFSKFTRLNMLRLARAVIPAAVLMMF
                     FPQLAMAAGSSGQDLMASGNTTVKATFGKDSSVVKWVVLAEVLVGAVMYMMTKNVKFL
                     AGFAIISVFIAVGMAVVGL"
     gene            complement(15620..>15823)
                     /gene="traY"
                     /locus_tag="ACHLAG_003640"
     CDS             complement(15620..>15823)
                     /gene="traY"
                     /locus_tag="ACHLAG_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HFP0656796.1"
                     /translation="RTRAAPGNKVLLILDETTNQKLLAARDRSGRTKTNEVFIRLKDH
                     LNRFPDFYNASLVKEEAESIDDI"
BASE COUNT         3679 a         4411 c         3819 g         3914 t
ORIGIN      
        1 gtatttataa tctcgttctg tacttctgat tttgagttca ggattatttt tattttctct
       61 cattataagt tccctctgat atttccgata attcattcag accagtcctg cggctgtaat
      121 atatttcaga caccgaaatc catcccacaa ttgcccccac aaaaaacaca acagaaacag
      181 aaaaggtaag gcacagcgaa acgacaattt ctacagggct ggcgggataa aaaagcccct
      241 gatggataaa ggggatattc aggctgacga ttattcccgt cgcgatgccg gatattccgg
      301 caatgagtaa caccacctgc ttatttcgca ccttttttcc ctccgtacat ctgcagaacg
      361 gtatccatcc gcgccataaa accggcggca tccgttgccc cctgtaaaag cggtaatgcc
      421 tcaagcgtgt tgacgttgac caggaaggtg gtcggtgtgg ccaccggaat attcgggaag
      481 aaggtctgca tcacgtccgg tggcaccggt aatgcttccg gaaaggcggt atccccctga
      541 ccatccaggg tgtagggaaa aacggaaaag ccgtactgct gtgccagctg cttcagtacc
      601 gggtcaaact ggtgacagta agggcaatgc ccctgcataa acagcaccac cttccagtcg
      661 gccaggttaa cctgcctgcc attactgagg cggaaccagc ggggagccgg ctttgctgtc
      721 ctggctgacg tgcctgctgc cggttgtgca ggctgcgtag ccataccctg cggattccag
      781 agtcgctcta tttcatcccg ggtggaggcc tgcacagcag cggcgctcag caacagggtg
      841 aacagcagtg atttagtgag agacatgtcc gccctcctgc tgcccttcca tcatggcctg
      901 ttgcctggct tccagtgcct cacgctcttc ccggctgaca cgggcaatcc atgcatccag
      961 cacctggtat gcgccgaaca gtacgagaat ggccgcaatc agctccgcca gaaaaaaagc
     1021 catccagggt ttgctgtaaa caagacgggc gacgatatga aaccagacac ccagggaaaa
     1081 tacccacatt cccgtaatat caagccgggg taaagagaat ctgcgtttac ttatcatgtt
     1141 aaactccggt tattatttac atgagagtat tcatgtctgt aatattcgcg tttgtgtgat
     1201 attaatcaga ggtacaaatg gaaaagcgat cttttaaaga aaaactggaa ataatcagaa
     1261 acataataag agagtcgtta ctgggaaatg cggcaattat tgcgctgata tatgcagcat
     1321 cacattcatt accggtcaat gccttccctg actatcttgt aataagttta ttaagtatcg
     1381 cagcagtcat cgttgtatta tggttatttt caattatcta tatttatttt tgtgagctat
     1441 tcagaagcca ctggattgcg gtatggttta tcatatggtc ttcagtaatc aacctcatta
     1501 ttttgtatgg tttttatgac cattttatat gacttagcct gtcataagcc cccttataaa
     1561 aaaccacatc actccggacc ataccgatga caacacaata cagataaata ttcttctgaa
     1621 cctgtcgaag ctcgtaacgt ccggtaatgc ggccgcaact attgaaacaa ccagtgctgc
     1681 gccctgactc ataattgcac cacacagaaa ccagagactg gcggtaagca catataaaca
     1741 cgcgatatta ataacaagga aaaccagtga gctcaggaga aacattttcc tgtcattacg
     1801 ctcagaaaag aaataaccgg caataatgac aaacattaac agaacaacac ctgtaaacat
     1861 tttcaaataa tcttcactca taaatcaaat ccgcgattaa aaattgggtt taaaatcttc
     1921 agaaacgttc aggaactgtt ttgccaggtc gtcctgcgaa ataaagccgt atgataacgg
     1981 gcgaacactg ccctgtttcg ggtcaaccag catcatggcc gggaaatatt tcacgccgag
     2041 gcgctgcgcc tgcccctggt cagtccggga atccggcaac agcggattaa tcacgccatc
     2101 cacggaaacg ggaataacac tcagaccata cgtatcccgg aagccattaa tgacctgcgc
     2161 cagttgcccg tcgatggggt cctgcccccg gtaaaaaaac atgatgccgt agtgttcagc
     2221 cagtttcgca atggcctgtc gctgctgcgc ctggtctgct gccagctggt tccgtaccgt
     2281 gccgttgtaa tggctgtact gcaggttata gtccagttca ggatgtgcca gcatggcctt
     2341 tctggcgctc atggtgaaaa gcccggcctg ctgagtccag taattttgca gccggaaata
     2401 tttcacaaaa ttatccacgc cgggatacag aatggcttcg tacagcgccc gctttgttgc
     2461 cgtctgcagt gcggccagtt tctgcataat atccggttcc tgacgggggg ctgcaggtac
     2521 aggtttattt tctttttcct tcggatttat tttctcgtta taccactgcc agcctgcatc
     2581 ttttcctgac gccggaaaga taaagcagca gagtaacagt ggcagtaatg ctttattcat
     2641 cttcattcct ttcatcactt tttctttcat caggtttctg caggtcagtc agggcagaaa
     2701 aacaaaaata cagaataaac aggagcacac cggagtaaag gataacgaaa gcagcggcaa
     2761 gcatcccgtc tgccgtggtt tcttttaccg tatcccacga ccagataatt accggagaaa
     2821 tgacaatcgc tgtaatcaaa agccggataa gaaaatcaat aaaacggttc gacgtgaata
     2881 tgactttcat catttccccc ggttatttcc cataccttac tgtcctgcct gtttcagctg
     2941 gtcagcaatc tgctctttca ctttctgcgt cagaacgcca ctgtccggga ttttctggtt
     3001 attcatcagg tcttcgtaga agttagtgaa atccagtctg ttgaactgaa ttttctgcag
     3061 ctcatcaacc gtaatccccc gacagtcagg atgcttcgca ctaccaaaac tgatacgcaa
     3121 ctgaccgttg cgcccctgct gctggactat ctgcgccagc ttcgaatcaa actgacagta
     3181 gctgcgtttt ttctccagac agacacccag cactttcttc gaacagaact ccccgacgct
     3241 gacggtcagt ttgtttgatt ttgctttagc cagggccttt tcatcactgc tgcatttggc
     3301 cagcccgata tcctgtcccc agccgctgtc cttacagcag ttgctgtatc cggctgcagc
     3361 cttcttacaa aatttcgcct gaccggtgaa agcacggaca tccaccccgt tcagcgccgc
     3421 aacatcctta cctgctgcgg ccagtgccgc cagctgagaa acggcttcgg caaaatcatt
     3481 actctgcccg ctctgagcct tatcgcattc gccatcgaga cagaagacgt ctcccccaca
     3541 gaccatcact ttcccggatg tttttgactc acaggagtac gtggcatatt catgcagaca
     3601 ggcaccttct tcggagtaaa aggcacactg gtgtgacacc agggtacagg caggattgtc
     3661 ggtgtaggtc tgacaggttc ccttgtctgc tgactgcgtg acatacgtgt cccggtatgc
     3721 ccagcagctc tggtacatgc tgtatgactg accgtctttc accagcgtcc tgttcccgcc
     3781 agcctccgta cattctgttt ttgtcagttt ccccagctcc ttgctgaacg ggcagctttc
     3841 agaccagaca acatacgggg tgtacgaggc tttttttacc ctgacaacca gacgcagctt
     3901 cattgccgta tgacgtttga attttgtatt cccgacccgg acggggtgag atgacgtaaa
     3961 agccacaccg gcagtcagtg ttttctgtac tggtgtaaat gaggcatctg ctgagtattt
     4021 ggcattccag ctcagtgggg ttcccagtac tgtaattgtc ataggtacac tcttctgcag
     4081 gtaagtacga ctccagctgt aatgcgcatc gacaatttca ccggtaacgg gagaggtaat
     4141 ggagacaaca tactgaccat tcacttcacg tgcaggtagc tgactcattt catactccag
     4201 cgtccgggtt tcccatgtgg tactcccctg cagctccata cgggcagttc gtgtgcagta
     4261 ctgctccacc tgtaaatccc gctcacaggt gtaattcgtg tattcactcc tgctaatctc
     4321 ctgcgcactg cactgctgcc cggtgtttcc cacaatgctg tcagcccggt tcaccacatc
     4381 cctgccggtc tggataaacg gtgcatccgg tgaaagaatg tctttcggct tgttcatgaa
     4441 tgactctgta atggtttttc cggtttcacc ggtcgcccat tccgtggtgc cgtcattttt
     4501 caggccgcca tccccgccgg cagtcacgcc accgtaatat tttgtctcgt ccggattcgc
     4561 gttatagccg gggatactct cctgtggctt aaagccctga atactgctgc ttccctgccc
     4621 tttgatctgg tgagcaaaat cagaaccggc ccggtaatca ctgttgctgt cagcctgtgc
     4681 catgccggca accagagcca gtatcagagg taaaatacgt ttcatttccc ggaatctcct
     4741 ttccctgcca gtaaatcatg agccacctgc cggcagtcac ccgtggcggc cactttctcc
     4801 agcgcctggc cgacccgcag gtttccccgg atgatgtcgt atccctgact gcagaacacc
     4861 accagtgccg gtacagtgcg aatgccgtac tgtgaaaaca gcgtcgggtc gatctgaaca
     4921 ccatcagtcg cgccgtcttt caccagggac aacacagctt cagcggtggt cttcaggtca
     4981 ttgttcacca tgccccgcag tgtggccgga ataccgtagt gccgggtttc gcccagcatt
     5041 cgtttcagcc cctcttcggg aatggaaaac gacacaaaat acagagcacc ctgccggggt
     5101 ttcccgtcct gcgaagcctg ctgtttacga accagctcat ccaggaaatg gttatctgaa
     5161 cgctgaaggg ggttttccag cacctgtttc tccgcccagg ctttcagctg atggtcaggt
     5221 ttttcacgca gttgtctgct taaattttcc tgctgcttca ggaactggcg gttttcagga
     5281 gtgttcacgt tttctgacgc cataaccgcc ccgttcagca tcatcagcag tgctgccaga
     5341 gatttcatac tcagcttcat cattcacctc acaggaagac acagttacgt ttacgccaca
     5401 tcaggtagcc gaagtttttc tttgtgttgg gcggattttt cccggtttcc cagcgggtca
     5461 cgctgcgccc gaacgggtgg cactgcccgc tgtccgggta catattcacc atctggtaac
     5521 gccagcgttc tttgggcagg attggggacg gatattcatt acagacggcg ttatttttcc
     5581 cgatggtttc cataatcatg ccctgacggt gcagcttgaa cgccatgcgt tcactgacca
     5641 gcagggagga ctgcaacgga ctggactcat tactcaccca gccattgaac gggtacatac
     5701 tgccctgcga accggcacac cagaacagaa catcgagagg catattaaag gcgctggcaa
     5761 tcgcatctgc tgcacaggcc ccctgtgcta tcggattggc aaagatgaca gcttccggat
     5821 tgagaatggt ggtcaggctg ctgtccgtcc aggtggggtc gatttcagaa agataagcga
     5881 tatccaggtc accaccttcc agacagccca gcgatgtgat gatgttaagc cagtacgtca
     5941 gcgggtattt gtaccagtga acgtgataga acgccccgtt cacctgcttc tcatcctttt
     6001 tcgccgttcc ctgtgccgtt ttaccaaaag ccggcaggct gaagcccagg ttcaccatgc
     6061 atcccggtga tcgggtgacg tccgtcagcg ccatcggctc ccagtaacca atggccagcc
     6121 cgatacgcct gaacagcggc ggcggtgccg gacaaatctg aatgggcatc gacgggttcg
     6181 ccgtgtcggg gaccttaccc tgactgactt tgatactgcc cagcgagagc gggaaaatac
     6241 agctccagca gatatccgtg atcgggttta caaaacgccc ctcacaggca gaatccgcag
     6301 acgctgcagg gacatgaccg gcgagaagga ataacatcag cagccacagg cttcgcttca
     6361 ttttctgccc tcctctgccg gaataaattc caccttcagg aagcgatcgc cgggaacggc
     6421 gctcacccgt gccgggacct gatcaatgcc gaggcgctgg cagagcacac cgttctggtc
     6481 aaagtaaaca cggctgtcca gagacttctg catctccggg atactgccct gcacgaggat
     6541 aattttgctc tccagtatgg gcggcgtctg gcgtttcatc caggccacct gcgccggatc
     6601 atcgccattg atgaaataca gcgtctggtt aaaaggaaca tactgcagcg gattcatcac
     6661 ttcaccctga cgggcaaaca cccgcccttc gttatcccgg atatccgcag ccagtctgac
     6721 ggacgggtca aacaaccggc tgccgtattt ctccgtgcgt ccgataccgg gcacggcagg
     6781 gggccgcaga ctgttgcgga tcacccgctc cttaaaggca tccattttcc ggcccatctc
     6841 accggactgc tccagtgccg tcaggcgctg catgatcacc gtcagcatgt ccggctcctt
     6901 taccggccac aaatcgcccc aggtaccaag atcggcggcg gccacactct gcccccatat
     6961 cagcagggca atcagccccc ggcatctcat ggttccgccc tcattcgccg ggcaatatcc
     7021 tgctggattt cccgggtgat atccggtgcc ccctgtacca cagcaggcga caccagaatg
     7081 accgcgtggt gtttctgctg ccacgcctgc aggctggctt ccagtgccgt gttaaaacgc
     7141 gctgaaaggg cttttgactg cgcttctgac agttgtttct gactggcgct gtcaaaaaac
     7201 gcatccacgg tctgtttcat gttgaaggcg acagtcaccg gtgcgttcag tctgacaatg
     7261 ccgtaactga cggctgcgtt cagtaacacc attgccaggc atcccggcac cgtccaccac
     7321 cagtggctgc gtcgttctgc cgtgacgtcg gcgggtttct gcgttgaact catgccacac
     7381 tcctgtattt ctcatgttct tccagccagg cttccagcga agccatttcc ggccctgact
     7441 tcttccacgc cagctgccac actgcctcat gaatactcag cccctcttta cgcttctgct
     7501 gcacaaactc aaaatccggg ccgtcagagc tgtacatggc gcggcttaac gggtccacaa
     7561 acagacggtg ccaggaggaa tggttttcca cctgcagcag gaaggaactg aaccactggt
     7621 ctttggccgc accaaactta ccaatcatgt cgcgctgcag tggcaggaac tggtccggat
     7681 acagttggtt gtatttcgcg aactccttcg cactctgttt gaggataatt ttgtaggagg
     7741 agttacccca tgccgcgcgg gcagcactgg aggccttatc agagtcaaag tcgacgatgt
     7801 tctgtgtgat ggtgatatag gcaccggtat gacggcgggc cgtacggtag cccttctcaa
     7861 taaattcacc gaccttgtga tttttgaagt ccagcagacg ccagccttca tcaataacgt
     7921 tcagtttctt gaggttacgc ggcgtgcggt acatcctgtt ctcgatgtag ataatcaggg
     7981 agaacatcac cgcaaccagc agtgacggac ggtcttccag tccgcccagc tccagcacca
     8041 ccatttttgc gtcatcccgc agggacggct catcagagtt aaaatactgg ccgtaagtgc
     8101 cgttggcagt gtactggtca agcagcacaa tcatttcgtc cagacggctg cggatagtcg
     8161 gtgactcggc atactgctcg ctgtcactgg cgtttttcag gaaatccacc acgtcatcaa
     8221 tacgtgctct gttctctttg gccagccagg aggctctgac cgcctgcaac agcagacctt
     8281 catgcacttc atccaggtta ccgttggggc tggccatcac tgacaactgg tcacggacac
     8341 gctccgctga ctggtcaata tcggtgatgt tcgcaaacgg gttaaaacgc agggtttcac
     8401 cgtccagata caccccgccc atgttctcac acagggactt gtatccatcc cccatgtcga
     8461 acaccacggc aaagcctccg gagtccagca cgctgcggat aagtggctgt atcagcccgg
     8521 ttttaccggc cccggaggtg ccacagaccg ccatgttgta gttggtgtta ttcatccccc
     8581 ggaagaaaat atcgataaac gccagctggt tacggtaggt gggtgccagc agacctgccg
     8641 gtgtcagggg gttatccgcc actaacggca tcaggttggc aacattaaag ctctctgcgc
     8701 gctgaaccac tccggcctct ttcagctgtt taaacagccc tttcccggcc ataaagggca
     8761 ggcaggtcag gaaattgcgc atgtggttaa agcgcggtga aatcagctca aaaccgtttt
     8821 tacgaaagct gttcaggatg tcctgctcca cttccagtgc cgtttcatta ttgtccttgc
     8881 agaaggctgt gatgttgagg aagtaggaca cgacagagga ctgaccggag cccagccgct
     8941 gacgcagttc cccccactcc ttcgcctctt tctccacgga cggaaaccat ttggcatagg
     9001 aggtcttcga ctttttctcc aggtccatgt acttcaggtt ggcttcgctg tgggttttca
     9061 cctggtcttc caccaccaga gtcagcgtca ggatgaacgg acaggagatg gacagttccg
     9121 ggttcagcag gttgctgtag ttgtcggcca tgttccacag gaaggcgatt tccgggttac
     9181 gggccagatg gaaattcagg atgcgggccg tgctgttcct gccgttctca cgcaggccca
     9241 gcgtcaggta atcagcccgg actttcagat caaaactgtc ctccacacac tgataattca
     9301 gatcagaata cgggtccagc tgacgtcttt tcgggtacag ggaatccggg ttatggttaa
     9361 tcatctcccc gacaatatcg ataaaggcct gtgcatccac cgtctgtgtg gtgatactgg
     9421 ccccctgtaa cgacgcccgg atgattttca ccaggttttc catttccaga atgtcggccc
     9481 ggcttttttt cttcgaggga gaacagtacg agataaacac ccggtaatgg cgcagggtca
     9541 ggggcagatt cattccctcc ggcagcggaa actgtgtcgc tgccgctttc atataatagg
     9601 cccgggtaat ggcgttaaac cgttcagcct gttcacctga ccaggagaac tcacgcagcc
     9661 cgtattcaat cctgtcacca accagctgac tggacatcag atggatacac aacggaatac
     9721 cgcgcggcag tttggtgcgc aacatgtgat cgagggcctc cacaatggac tcattcgccc
     9781 cattgatggg gatggcttcc agcataaagc ccatcgtggt gtcattcatg aacagaccgg
     9841 attcctggtt gtaatcacgg tacggcagca gacggctgaa ctgcgggaag ctcatttcgc
     9901 ccagaacttc attggccttt gcggattcgt caggcagttt cagtgctgtg acgagggagt
     9961 taaccgcctg agtgacggcc tcaagtgggt tattcacatg aattccttag ttgaaaaata
    10021 aagcgagaaa tgcggtagcc atgaagggaa tacctgtaat taacgcgcca tttcccggcg
    10081 catatacaaa gatttctgtt ttctgaatat ccactcatgt tcatccggaa ttaaatcact
    10141 atgttctgcg actgacttat gcataatgtt ttctctgtct ttcctgatac gcctgacatt
    10201 caacgcacaa cgtcacaccg ggaaatattt tccgccgggc ttccggaata ggatttccgc
    10261 atgcttcaca gagataaaca ggaataccat gagcatttat tttctggcgt atccggttta
    10321 ttcctgtcat ggtcagttgt tctgtcactg aatatgcttc atcggcttca tcactcacgg
    10381 aatattcctc ccgacatgtt ctgacacgct accgcccatc ccttttcacg cacagggcac
    10441 tcgttttaca ggcagatttt gaatacagaa agcccgccgg cattgccggc tgatatacaa
    10501 cttcagggcc agttaattaa tacgtggttt tccccacgca gacggtttaa taacaaaaaa
    10561 cacgctggat ggctgatgat aaacatcctg gttatcaata taaggcgcta tccataatgc
    10621 tgccgtctgc tcacccgttc ttagcggacg aggaggtgtt accggcgtaa ctgaactgac
    10681 cggaacaacc gttttcactt cccgggcagc ggtaaacagc gggcgaggtg ccagcagttt
    10741 ctgctccgga tgtgctgtca ccgcaggtct gctgccggac ggagtggtcg cggtgacggt
    10801 ctgcaccggc attgtccgga agttcccttc agccaggcgc ggcagtgatg ccgcaaccgg
    10861 ctttgcttct gaggactgct ccagtttttt ggccttctca ttggcctgct ccatcgtcat
    10921 acaggtatcg gatgtggtgg cgttacactc aaattccgta ctggttccgg cacagccgga
    10981 aagtaataag gtccccagca gaggaataaa gaaagaaatc tgtttcattg tccctcacct
    11041 ttctcatcgc agttcacaaa atccttcttc aaatccctga ccgtacaggt ttttacaatg
    11101 tcaggatttt caaaaaccgt actccgtgat gatttataaa catatgccac aatgcgtttc
    11161 ccacccgcat caggttctgg tgcactgact tcacataacg gaggtgttat tccggactca
    11221 tacagcaccg gataatttat ttttttgacg ctgccatcag aaaccagctt attcatcgaa
    11281 gggctcctta tatttctgaa taattctgtc gattagttct ttcaccggaa cagattttcc
    11341 gccaccggat tgtccggcaa ccacaatatg ccccggagaa gaaaataaac tcatcgacat
    11401 acccttcttc atcacaccga cagcagccat attaaaattg tccggcaatg actcagaacc
    11461 gaatttctcc ggtgcaggaa gtacgattct tgtggcgcag ttactgagaa cagcatgcat
    11521 aaaatcatct gacacggttt tacccggcac agagagggct gtaataacat taatattacg
    11581 catgttcatt tttatctccg ttgtttctgt ttgttctgtc atcaggaaga taaacagggc
    11641 atttcctgat ttttcccggc agggaagcgt cttccgtata aacttccttc agatgttcat
    11701 ctgcggttac cacccggata catttcattt ttccgggtcg caccggatat ttcccggtga
    11761 tcgctgccag cgagacatac atcagtgcca gtgttgccca taatgcggtg accagttgca
    11821 tcagtgaccg gtcagtctcc ctgacataat ctgcagcatc agccgcgcgg taatggcaga
    11881 tatcctgtat ttctgatctg cctgaagaat cagaggaaga aaaccagcat tcactgaccg
    11941 ggaactgtcc ggcaggtgct gtttgtctta cgaagttaat ctccttaacc agtaatttcc
    12001 ccggcgtggt gttgtccttc cataaaacaa acagagccat cagggccatt gtggccagtg
    12061 gcagaatgac ggtgtacttc agacaccaga acagtcctcg cagaacccgg gcgacaacat
    12121 accgggcggc atggcacgcc tgacgggacg acatattatt tgccatcgtt gccccctccc
    12181 tggctgccgg acataaactg ctgcggattc aggttgccca gctgcttcag catctggtca
    12241 gtattaaagc cgttaagatg actttcagcc gatggcggaa caggaaccgg actttccgga
    12301 ttgtcttctt ccgctctgct ctgcgtgcgt tccagcgcca tctcttccac ggttttcagc
    12361 tggaagccgt cctggaacac cacggtcact tcgttgcccg caccaatcgg tatcaccggg
    12421 tgatactgtt cggcacgttt gatgtagtag tcactgagcg tctgtgcggc tttcgatgcg
    12481 ccgccaccga tacccatttt caggacatca ccagccccgt aagcggctgt ggcacccagc
    12541 ccgacagccg gctgggaggc gcgctccatt ccctgaccga taccgtcaac aaatcccgcg
    12601 ccccatgccc agccgaggat tttgccgtta cgcatcacca cttcgccctt gataccgttt
    12661 ttaccccgga agctgacatg ccccttaatc ggcatatcaa tagttttgcc gtccttcagg
    12721 caactgatat tgcgggtgcg tacaatggca cgctcactgg acacatcccc ccaggcttcc
    12781 agacccacaa aacatcccgt tgcgtcatac gtcttgctgt tcggcatttc caccaggccg
    12841 gtgatacgca gctgcatcgg caccgtggat tcattaccgg tgacagaggc attggcatcc
    12901 gccccttcaa tcagcatggc tttcgcaaaa cttcctgacg gaatgtacgg cagtgatggt
    12961 ccctgttttc cctcattacg ggtaaacacc ttacgctgta tccggttagg caccggcaca
    13021 gactggtacg tcacctgtgg tggtggcgtg acaccattcc cgggataaaa cgccgtcggc
    13081 ggtggaacag caacgctgcc ctgcggcggg aaggatacgg gggtgtttcc tggctgtggt
    13141 tcgccttccg ggccgggcgg tgaggcaggc atctgcggta caggctcacc ggtcgccgtg
    13201 acgggattag cacccagggc ttttacctgc tctgccaggg cggcattgtc ctgtcccaac
    13261 ttttcaatac gacgctggtc atcaccgcgc tgtttgttca gaacatccag ctcacgacgg
    13321 atttcctcat actgcttctg catctgcgct gccgtcacct gcatctcggt ggtggcatgc
    13381 tgacgcacct tgtcatcaaa ggtcgtatcc accacaccgg tcatatccgg cacaggctcc
    13441 tgttcagcca cggtttcacc gttaccggac atgtccacat cagacagata cagtgcccca
    13501 ccaatcgcgg aggctgtacc gacaaccaca atccccagcc acaggtactg cttgcgtttc
    13561 acaatggtat tgatactggc catcagttgc cctccccgtc acggatgaca tacacatcca
    13621 tgcgcgcacc tgccagtaac tggctggcag gctggctgaa catcacggca cgggttcccg
    13681 gctgccagaa gtcactttcc cggatattca gggcagacag cgttttattt tccacggcaa
    13741 agcggaccat cttcaggtga ttccccgtcc atacggcatc tgccactgaa gacagccccg
    13801 ccggggcctg cagggtttcc tttgtcactg ggacctgata ccagcctgcg ggtaattttc
    13861 cgccacggac ggcctggctg atggtcacaa gcagggattc gtaaggcgtg gacgtttccc
    13921 acgcaccggc ttcttctccg gttccgcgca ggtcactgac cagctgaatg gtacgccccg
    13981 cgccttcacg gggaacggcc tgaatggaaa gattcagacc acgttctgtt tccagaatga
    14041 acgtaaaggg ctttttgttg acggtggcaa ccaccacacc gccggaggcg gtctgctcat
    14101 tattggtcag ggcaccatcc agactgttca cggcgataat ccggtcaccg ggaacggtaa
    14161 acagattcgg gctggtattg ctgatggcaa tactggcctg accaccgttc accattggca
    14221 ccacggtggg ggccagcgta ccgtttgcgg ccatcacgct gcaggaaaaa aacaggctgc
    14281 cgaatattat tgccgtatta ttttttctca tcgtctgttt ccccgaaatt atccagccag
    14341 gtcaccccgt tttcccgctt cagaataagg atgtaatgtt tgatatcagt gaaaggtttt
    14401 gaatcaccaa tccaggtttt aagcacacct cgaatttcca cacggccata ctgaggccag
    14461 acacgaacac tggtctggaa aaaggctgag ttcacgttat cgtttttaat acgcttcgct
    14521 tcttcagcca gaataacttt catctggttc tgtgcgcccg ggcggatata ttgcagaagc
    14581 gcctgatgtg aggcatcggc ggtttctgat gaaacattca gacggagggc aataaatgac
    14641 agcgccatct gctgtaaata agaggcgtcg gcactgttct gtgacacggc aaagggggca
    14701 ttaaatgcca ttggcgtcac ggcagtgcgt tgctcattct gaagacggta gttattcacc
    14761 ccctaaatga cgttaacaga gaggctgaga acaataagca ctgacataaa tataaaggcg
    14821 atggccatta cacgactggt acttaaacgg gcaccgtgtt ccatataaca atcctggtat
    14881 cagttctatt taatccactg ccggaaacac gaatcgggaa cattatgaaa aataccgcgc
    14941 agcagggctg ttggcatata ccagtaaatc aggtcacgta accaggaact gccccgccct
    15001 tttttcagtt ttttaatccc gaaataaacc agaaccgctg caccaatacc gaacagatat
    15061 ttcgatgttg tgatacccca gccaatacag attgctgcgg ggatcagttc atccagtggc
    15121 aggccaaacc agcggctctg gttggtcagt gtttccggga aacgatattt cttaagttta
    15181 ttctcgtctc ccgacatcgt tttatttcct gtcagaggcc aacgacggcc atacccacag
    15241 caataaatac agagatgatg gcaaaaccgg ccaggaactt gacgtttttg gtcatcatgt
    15301 acatgaccgc accgaccaga acttcagcca gaacaaccca tttaacaaca ctggagtcct
    15361 taccgaaggt cgccttaacc gtggtgttac cacttgccat caggtcctga ccactgctgc
    15421 cggcggccat cgccagctgc gggaagaaca tcatcagaac agcagccggg atcacagcgc
    15481 gagccaggcg aagcatattc agacgagtga atttagaaaa aaacgacttc tttttgacgg
    15541 gcgcagaaac accctgaaca cttaaaacgg cgtccatatg attcctctgt ttaatattga
    15601 tagtgaaaaa taaaactctt tatatatcgt ctatgctttc agcttcctcc tttactaaag
    15661 atgcattata aaagtctgga aaacgattga gatgatcttt tagtctaatg aacacctcat
    15721 tagtttttgt tcttccgcta cgatctctag ctgctaaaag tttctgattg gttgtctcat
    15781 ccagtattaa taagactttg ttacccggtg cggctctggt tct
//
LOCUS       DAZGVQ010000117        15817 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000117 DAZGVQ010000000
VERSION     DAZGVQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15817
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.118"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..263)
                     /locus_tag="ACHLAG_003641"
     CDS             complement(<1..263)
                     /locus_tag="ACHLAG_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656797.1"
                     /translation="MSKVVRIIFEYKEHVIHKNADGTVRMGVSLDIRSTGIKQKGDGP
                     AMIFGVVMLAESRYFAELVAMKASALMKDMSMRSGVIKGNEFN"
     gene            complement(338..565)
                     /locus_tag="ACHLAG_003642"
     CDS             complement(338..565)
                     /locus_tag="ACHLAG_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656798.1"
                     /translation="MNLFDSLNNARRLTELAGAVLERSKRYPQRFALKTTPPVNEVKG
                     TGEIEITVKTNGLRRRVKATRISGCTVYWEV"
     gene            complement(983..1204)
                     /locus_tag="ACHLAG_003643"
     CDS             complement(983..1204)
                     /locus_tag="ACHLAG_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656799.1"
                     /translation="MIAFNIPDIYGRFHLVNFDNVKAVSVSDSEECGDLLFEFNDRTR
                     MAISAGLDREGAKEAYSNICRFIGAKRIS"
     gene            complement(1207..2139)
                     /locus_tag="ACHLAG_003644"
     CDS             complement(1207..2139)
                     /locus_tag="ACHLAG_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0656800.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNDAITAAMLSWYENWNKQSALSELPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELAEELGIDYTRANKGALSRAI
                     RRRLMGKRGLVIVDEADHLGIDGLEQLRAIQDATGVGMVLIGNPKGLFKGGRRAVDDL
                     SRLFSRLARTKQLRKAKKADVLAIARAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWITASGEGAALTEKHINAAFKEVYANPELLSQV"
     gene            complement(2221..4311)
                     /locus_tag="ACHLAG_003645"
     CDS             complement(2221..4311)
                     /locus_tag="ACHLAG_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFP0656801.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRKVAEARIAIVSEVLKIAQQPGFSCAKAIRFLVNSLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADVLDKEIVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFSALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPMKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRLDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVINVLEHKPQEPWLENIYRPMGNTVAVQQPAIDDEPDEEYERNFQRGL
                     QLLEAKLKENDPLA"
     gene            complement(4313..4561)
                     /locus_tag="ACHLAG_003646"
     CDS             complement(4313..4561)
                     /locus_tag="ACHLAG_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0656802.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            4752..5282
                     /locus_tag="ACHLAG_003647"
     CDS             4752..5282
                     /locus_tag="ACHLAG_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656803.1"
                     /translation="MSQGKEPVIERIGMLTKRYPSRSAAARAWGININTLNSYFKGGQ
                     TQPAPRESILIRISESEGVSLEWLKEGKGELPNDIGIYGNFGCSGDKGDQLTQMLSFL
                     TKDEREQLTNLLARKGVETVLTLLNKENQDLLQLEGRKRLAALMIVDMPDDCVREILE
                     KTETSRQSNPVESKAG"
     gene            5533..8814
                     /gene="traI"
                     /locus_tag="ACHLAG_003648"
                     /pseudo
     CDS             5533..8814
                     /gene="traI"
                     /locus_tag="ACHLAG_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            8834..9580
                     /gene="traX"
                     /locus_tag="ACHLAG_003649"
     CDS             8834..9580
                     /gene="traX"
                     /locus_tag="ACHLAG_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFP0656804.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRIRHLDQSWMFLVGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWAMIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRTERLVLVACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSR
                     VGKSLPRFWPGDFFPTFYGCHLAVLGILAL"
     gene            9639..10499
                     /locus_tag="ACHLAG_003650"
     CDS             9639..10499
                     /locus_tag="ACHLAG_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0656805.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGEVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDDESKVFFEKIKARHPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVVA
                     VQTQWFKQYL"
     gene            10602..11159
                     /locus_tag="ACHLAG_003651"
     CDS             10602..11159
                     /locus_tag="ACHLAG_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFP0656806.1"
                     /translation="MAEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVSQRNIPLSHKKLRRALKAITRSESYLGAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELRAILAE"
     gene            11314..11517
                     /locus_tag="ACHLAG_003652"
     CDS             11314..11517
                     /locus_tag="ACHLAG_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656807.1"
                     /translation="MAVSSGLSEHSSIYRYQREGRHAVRQEIRGFFREAAEVWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
     gene            12260..12721
                     /locus_tag="ACHLAG_003653"
     CDS             12260..12721
                     /locus_tag="ACHLAG_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFP0656808.1"
                     /translation="MKKYIPVVLFVFSCQVFSADIHGRVVRVLDGDTIDVMLSQHPVR
                     VRLVNIDAPEKKQEYGRWSEKIMKSLVAGKTVTVTYFQRDHYGRILGQVYAPDGMNVN
                     QFMVRAGDAWVYEQYNTDPVLPVLQGEVRQQKRGLWADSAPVPPWVWRSRK"
     gene            12824..13207
                     /locus_tag="ACHLAG_003654"
     CDS             12824..13207
                     /locus_tag="ACHLAG_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656809.1"
                     /translation="MNYQAVSELITSSNHNVLIVGESASEVDSFLNKLNITDYKYYDF
                     SQIYSCSDRTLNDYAVIFIRNALNASEHIIIFNCTGWSDLNNESAVMQFARVARKSGK
                     QLIVAVREQDMKKMEAEFGRIIKIH"
     gene            13487..13636
                     /locus_tag="ACHLAG_003655"
     CDS             13487..13636
                     /locus_tag="ACHLAG_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0656810.1"
                     /translation="MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLA
                     YEARK"
     gene            13920..14168
                     /locus_tag="ACHLAG_003656"
     CDS             13920..14168
                     /locus_tag="ACHLAG_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFP0656811.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKAQLQEMCEAEGVTQAEMIAELIKQKSAFS"
     gene            complement(14241..14384)
                     /locus_tag="ACHLAG_003657"
     CDS             complement(14241..14384)
                     /locus_tag="ACHLAG_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005023896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HFP0656812.1"
                     /translation="MVGYAVLWGPGNLLVAKHRKIIGVFAFLAPVHPHQNQFPATLWR
                     GQP"
     gene            14410..14484
                     /gene="tap"
                     /locus_tag="ACHLAG_003658"
     CDS             14410..14484
                     /gene="tap"
                     /locus_tag="ACHLAG_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFP0656813.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            14477..15334
                     /gene="repA"
                     /locus_tag="ACHLAG_003659"
     CDS             14477..15334
                     /gene="repA"
                     /locus_tag="ACHLAG_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFP0656814.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPRKGAGTLKFCEKLMEKAVGFSSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDV
                     SEDAVAAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREIAEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT         3901 a         3876 c         4234 g         3806 t
ORIGIN      
        1 tgattaaatt cattaccttt aataaccccg gaacgcatgc tcatatcttt catgagcgca
       61 ctggctttca ttgccacaag ttcagcaaag tatctgcttt ccgcgagcat aaccacccca
      121 aaaatcatgg caggtccatc acctttctgc tttatcccgg ttgaacgtat gtccagactt
      181 acccccatgc gcactgttcc atccgcgttt ttatggataa cgtgctcctt gtattcgaaa
      241 ataatgcgta cgactttact catcctctgg tcctcgtttt gttcggataa ctgacccggc
      301 gtctgacctg atgacagagc cgaaccatga agcaacttca tacttcccag taaaccgtgc
      361 atccgcttat acgggtggcc ttcacacggc ggcgcaggcc gttggtctta acggtgattt
      421 caatttcacc ggttccctta acttcgttta ccggcggcgt ggttttcagt gcaaaacgtt
      481 gcgggtagcg tttgctgcgc tccagtaccg caccagcaag ttcagtcagg cggcgggcgt
      541 tattcagtga atcaaacaaa ttcataggga actccggttg tatcagtaat ggattcatgc
      601 gcaatgcagc cagctctgac aggaagcctc gattaacttt acccatcccc ggaatttttc
      661 gggtttcgcc acgcatgatt gcacgaagaa tggagtaacc gtgtcgccac ccctggcgat
      721 aatcatccat aatcagaaaa ccaaagcgca ggctttccca tacatccaca tcaaagcatt
      781 catgattaaa acggcgtctg atggaatcaa gttgcatgtc tgcataccac tgatacaggt
      841 ctatatacag aaaacacaca ccatcaccga tcatcaattc atagatataa tcacgaacct
      901 cacagccatt gtcgttaaac tctcgaaagc tgtggagaac attgatttcc tgctgtatct
      961 gcgtattcat ataacacctc gtttagctga ttcgttttgc accgataaaa cgacaaatat
     1021 tgctgtaagc ttctttcgca ccttcacgat caagtcctgc cgatattgcc attctggtgc
     1081 gatcattaaa ttcaaaaagt aaatcgccac attcttcact gtcagacacg gagactgctt
     1141 tgacgttatc gaaattcacc aggtgaaagc gtccgtaaat atcaggaata ttaaatgcaa
     1201 tcataatcac acctgtgaga gtaattcagg gttggcataa acctctttaa aggccgcatt
     1261 aatatgtttt tctgtcagtg ccgcaccttc accgctggcg gtgatccacg cctggttaag
     1321 cgtatgcgtc agaacgcgta atgcccccgg tttttcagcg atagcctgca tgacagccag
     1381 ctcggcctca ccactaatcc cccatgccct ggcaatagcc agcacatccg cttttttggc
     1441 cttgcgtagt tgttttgtac gggcaagacg gctgaacagg cgagataaat catcaacggc
     1501 gcggcgtccg cctttaaaca gcccctttgg gttaccaata agcaccatcc cgacccctgt
     1561 ggcgtcctgg attgcccgga gttgctccag accgtcaata ccaagatgat cagcctcatc
     1621 aacaatcacc agtccgcgct ttcccatcag gcgacggcgg atggcgcggg ataatgcccc
     1681 tttgttcgcg cgggtgtaat caatccccag ttcctctgcc agctccagca gacactccgt
     1741 gacgctggag tgcgcgggtg acagggtgat catccaggtg ttcggttgct cctggcagta
     1801 attacgggcg gtggccgttt tacccacacc cggcacgccc actatgacgt taatacagcc
     1861 catcaggcga accgcctgaa acagtgcgcg cagctcctgg actgtctgcg tttccacaaa
     1921 ctgtggcagc tcggacagtg cgctttgttt attccagttt tcataccagg aaagcatggc
     1981 cgccgttata gcatcattgt ctcctttata tttccctttg cggaaagccg ataatgtgcc
     2041 gtcggaaatt cccgcctccc tggcgatggc atactgcgtc agtacgccgc catcaataag
     2101 ttcatctatg gtcttgatta catcgttaat atcagtcata ttatttacct cgcttgagac
     2161 agcattctcc gagactgaaa cccaaataac ctgagttacc cctcgggtta tttttttatt
     2221 tcaggccagc gggtcatttt cttttaattt cgcttcaagc agctgtaatc cccgctggaa
     2281 attacgctcg tattcttcgt caggttcatc atcaatcgct ggctgctgaa cggccaccgt
     2341 attacccata gggcggtata tgttttccag ccatggctcc tgcggcttgt gctccagcac
     2401 gttgataacc tcatcctcgg catcacgaat tttttcctct gcgcgtttac gcatgccttt
     2461 aagacgctgc tgctgtttgt agtattccgc gctgaccggg aaggcttcgc gtttattgcc
     2521 gtcccatacc gcctcgcaaa tcacgctgcc atccaggcga cgcacggtaa ttcgttcggc
     2581 atcatgaatg tcatagctga taagcacttt gcggccatgt tcatcacgca gctcgggcgc
     2641 gtagtaaata ttattcagcc agcgtatttc acagcgtctt acagggcgtt ccaccatcgg
     2701 ccggaacatg tcccgcaatt ccacatcgga cagccattca atttccgtgt cctcttccgc
     2761 caggcgtttt tttctgaact ccgccgggct gtaatgttta ccgttcggct tcatgggtaa
     2821 ttcatcgtgc ggccggttgt tgtaccactc aacaccgtca cgaatggcat caatcagttc
     2881 agaccaggac ggtaaatcac gcatagctga ctgctgccgg gcgttcagcc gtttgccctg
     2941 ttgcagggca ctgaatgccg agcgtaaatc gcggttggtt ttacgtaacg tctcgcgatc
     3001 tgctcctttc ccgaaatagg tgcggtattt acgggctatg cgcatcggta atgtgcggtt
     3061 aagccgttcg ataatgcccc gtccctgcgg attaccggca atccctgtcg ggtgattaat
     3121 ccccagtcgt ggcagtatcc ccacaatctc cttatccagg acgtcggcgg tttccccgga
     3181 gccattatcc gagtaataca gaaacggttt gccatgatgg cgaataccgt gctgtatggc
     3241 accggctacg gcgaaaacat tttcagccag gtcaaggctc cagcccacca caaagcgcgt
     3301 gccaccgtcg ataacaaagg tcacttccgg tgcgaatggc cgcccgtgaa ccgggtgcgc
     3361 gcatttcagc ttcatgccgt gaccgtcacc aatccagaca taattcaccg gcattctgga
     3421 ccagtcgcgg cgcgtgaatc cctcaagctg acggtattca ctgccagtta cccggccttt
     3481 ttgtttcacc acttccggca gtttcttcat tgcgcggcga atggtgtcat aagagggcat
     3541 gatatcgagc atataaggct catcagcgtg ccggtgctgc cattcagcaa caaaatcctc
     3601 gtaagcctcg gtcattggtc ggccgtttga ctggcgatac tgcgccagaa attcgggcag
     3661 ccagttaata tcttcggctt ttatttcctg gcgtttaccc ggtgccagta aaagcaggcg
     3721 ttcagcggcg ttctgtgcct tgttaaaggc cgcaatccag cgtttcagcg tgatttcact
     3781 caacgcgcgg ctgtttccct ttttggcgtt cgccgtctca accattgcca caatgcgctc
     3841 gtccagttgt gatcgtgcca ggctgttaac gaggaaccgg atagccttag cgcaactaaa
     3901 gccgggttgt tgcgcgattt tcagcacctc gctgacgatc gctattcgtg cctctgccac
     3961 tttacgttgg ttttcagtca gggcattgag gcgttcgacc atcagttggg gtgatccgcg
     4021 atatgcctcc accgcatcaa ccacggcagg tgagcgtctg gcctttgtca ccaccggagc
     4081 cggtgattca tcggcttttt gcgtcatcag ttgcagggcg tagcgttcac gtaatgcctg
     4141 ctgcgtcact tcaggtaaac agtcgatgct gtattcagtt gctttagagc ctgagcgacg
     4201 acgggaacaa cagcttttcc cttgtacgta acgttgtaat gcctggcgaa taccttttgt
     4261 tgtagcaggc attccgggcg cgccaatcag ttcttttgca atgacataca tatcatgacg
     4321 cctttctcat atagctctta acctggtatc gactgggcca gatttcttca ggtgataccc
     4381 caagagcatc agcaattatt tgctgatacg gtcggcaagg agtacgtaat acgcttttca
     4441 gcgaatccct gctgtaacca gcctttaaag ataaagagcg cagtgataag ccagccatgt
     4501 gtaagcgcgc cttgatgatt tcaggcggcc aatcgtgatc tgaaacttct cttctactca
     4561 tacttcctct attctaaaaa gttacccgcg cggatattcg tgcggatatc tgtacgggta
     4621 aataatgcat caccaaaaat accaaatcaa ggtatttttg gtattttggc taatttttgc
     4681 ttttatcctt atttttcaat tggataaata gaaagaaacc tttccttaga aaagcaccaa
     4741 gaggtatctt tatgtcgcag ggaaaagaac ctgttattga acgtattgga atgcttacaa
     4801 agcgctaccc ttcaagaagt gcagctgcca gagcgtgggg aatcaatatc aacacattaa
     4861 acagctattt caagggagga caaacacaac cagcccccag agaaagtatc cttatacgaa
     4921 tttctgaaag tgaaggcgtt agtcttgagt ggcttaaaga gggcaagggt gaattaccaa
     4981 acgatattgg tatttatggt aattttggtt gttctggtga taagggagat cagcttacac
     5041 agatgctttc attccttacg aaagacgaac gagaacagtt aaccaatctc cttgctcgca
     5101 aaggtgtaga gacggttctg acactgctga ataaagaaaa ccaagattta ttacaactag
     5161 aaggcaggaa gcgcctggct gcgttgatga ttgtagatat gccagacgat tgcgtcagag
     5221 agattttgga aaaaaccgag acaagtaggc aatcaaatcc ggtagaatct aaagcgggtt
     5281 agtttggcga ggctggcccg tatgttagtt gccatataga tttaatgaag ttttaaacgc
     5341 tttaaaaagt tgagatcgca gtatcaaaag ttttgccgat tttagaataa acgattaatt
     5401 attgcacaat cgatatcaaa tagactttaa gcgctcatcc agacatcacc tgttttattt
     5461 cagtatctta caggtgattt cacctccttt cactaaaacc ctcgttggta tcaaatgatt
     5521 cacctggtta cacgcagtca tgaccgctat gttatcgacc gggtgacggc gcagagtcac
     5581 agcctgaccc tgcgggatgc gcagggtgaa acgcaggtgg tgcgtatttc ctccctggac
     5641 agcagctggt cgctgttccg gccggaaaaa atgccggtgg cagacggcga gcgactgagg
     5701 gtgacaggga aaattcccgg actccgcgtc tccggcggtg accgcctgca ggtggcatcc
     5761 gtcagtgaag atgcgatgac ggttgttgtg ccggggcggg ctgaaccggc caccctgcct
     5821 gtggctgatt caccgttcac ggcactgaag ctggagaacg gctgggtgga aacgcccggg
     5881 cattccgtca gcgacagtgc gaaggttttt gcctccgtca cacagatggc aatggacaac
     5941 gccaccctga acggtctggc ccgcagcggt cgtgatgtcc ggctgtattc ctcactggat
     6001 gaaacccgta ctgcggaaaa acttgcccgc catccctcct ttacggtggt ttctgagcag
     6061 ataaaggcgc gtgccggtga gacatcgctg gaaacggcta tcagtcatca gaagtccgca
     6121 cttcacacgc cggcacagca ggccattcat ctggcccttc cggtggtgga aagtaaaaac
     6181 ctggccttca gccatgtgga cctgctgaca gaggcaaagt cgtttgctgc agaaggaacc
     6241 agttttactg aactggggcg ggaaatcgat gcgcagataa aacggggtga tttactgcat
     6301 gtggatgtgg ctaaaggcta tggcacagac ctgctggttt cccgtgcgtc gtatgaggca
     6361 gaaaagagca ttcttcgcca tattctcgag ggtaaggagg cggtcacgcc gctgatggag
     6421 agagtacctg gcgaactcat ggagaaactg acatcaggac agcgtgccgc cacccgcatg
     6481 atactggaaa cgtccgaccg tttcacggtg gtgcaggggt atgccggggt gggtaagacc
     6541 acacagttcc gggcggtgat gtcagccgtg aacatgctgc cggagagtga gcgtccccgt
     6601 gtcgttgggc tggggcccac gcaccgtgcg gtcggggaga tgcgcagcgc aggcgtggat
     6661 gcacagacac tggcgtcctt tctgcatgac acgcagctgc agcagcgcag cggagaaacg
     6721 ccggatttca gcaacacgct gttcctgctc gatgagagct caatggtggg caataccgac
     6781 atggcacggg catacgccct gattgcggcc ggtggtggtc gtgccgtggc cagcggtgac
     6841 acggaccagc tgcaggccat cgcgcccggt cagcctttcc gtctccagca gacgcgcagt
     6901 gctgccgatg tggccatcat gaaggagatt gtgcgtcaga cgccggaact gcgggaggcg
     6961 gtatacagcc tgattaaccg ggatgtggaa agggcactgt ccgggcttga gagtgtgaaa
     7021 ccgtctcagg tgccacgtct ggagggcgca tgggcaccgg agcactccgt gacggagttc
     7081 agtcacagcc aggaagcgaa actggcagaa gcgcagcaga aagcgatgct gaaaggcgag
     7141 gcttttccgg atgtccccat gacactgtat gaagccattg tccgcgacta taccggcagg
     7201 acaccggaag cacgggagca gacgctgatt gttacgcacc tgaatgagga ccggcgcgtg
     7261 ctgaacagca tgattcatga tgcactggaa aaggccggtg agctgggaaa agagcaggtc
     7321 atggtgcctg tcctgaacac agcgaatata cgcgacgggg agttgcgtcg tctctccacc
     7381 tgggagacac atcgggacgc acttgccctg gtggataatg tgtatcaccg gattgccggt
     7441 atcagtaagg atgacgggct gataaccctg gaggatgcgg aaggtaacac gcggctgatt
     7501 tcgccccggg aggcggtggc tgaaggcgtc acactataca ctccggacac catccgggtg
     7561 ggaaccggtg accggatgcg cttcacgaag agtgaccggg agcgcggtta tgtggccaac
     7621 agcgtctgga cggtgacagc ggtttccggt gacagtgtca cgctgtcgga cggacagcag
     7681 acccgggaga ttcgccccgg ccaggagcag gcagagcaac atattgacct ggcctatgcc
     7741 atcaccgctc acggtgcgca gggggcaagt gaaacctttg ccattgcgct tgaaggcacg
     7801 gaaggtaacc ggaaacagat ggccggcttt gagtcagcct atgtggccct gtcgcgtatg
     7861 aagcagcatg tgcaggtgta caccgataac cgtcagggct ggacggatgc catcaacaat
     7921 gccgtacaga aaggaactgc ccacgatgtg tttgagccga aaccggaccg ggaggtcatg
     7981 aatgcagagc ggttgttcag tacggcgcgg gagctgcggg acgtggcggc aggccgtgcc
     8041 gttctccgtc aggcggggct ggccggggga gacagtcctg cacggtttat tgctccggga
     8101 cgtaaatatc cgcaaccgta tgtggcactg ccggcttttg accgtaacgg caagtccgcc
     8161 ggtatctggc tgaatccgct gaccacggat gacggaaacg ggctgcgggg cttcagtggt
     8221 gaaggccggg tgaaaggcag cggggatgcg cagttcgtgg ccctgcaggg cagccgtaac
     8281 ggagagagcc tgctggctga taatatgcag gatggtgtcc ggattgcccg tgataatcct
     8341 gacagcggtg tggtggtaag aatcgccggt gaaggtcgtc cgtggaatcc cggtaccatc
     8401 accggtggtc gcgtgtgggg ggatatcccg gacaacagcg ttcagccggg agccggaaat
     8461 ggcgaaccgg tcacggcaga ggtactggca cagcggcagg ctgaagaggc catccgccgt
     8521 gaaacggaac gccgtgcgga tgaaattgtc cgtaaaatgg cagagaacaa acctgacctg
     8581 ccggacggca aaacagagca ggctgtcagg gatattgccg ggctggagcg tgaccggtct
     8641 gccatatctg aacgggaagc cgcgctgccg gagagtgtac tgcgtgaacc acaacgggtg
     8701 cgggaagcgg tccgggaggt tgcccgggaa aatctgctgc aggagcgact gcagcagatg
     8761 gagcgggata tggttcgtga cctgcagaaa gagaaaaccc tgggcggaga ctgacacagg
     8821 aagataaacg ctgatgacaa cggataacac gaacacaaca cgtaatgatt cactggctgc
     8881 ccggaccgat acctggttgc agtcgttgct ggtctggtca cccggacagc gggatatcat
     8941 caaaacggtg gcactggtgc tgatggttct ggaccatgca aaccgcattc ggcatctgga
     9001 ccagtcgtgg atgtttctgg ttgggcgtgg ggcctttccg ctgtttgccc tggtgtgggg
     9061 gctgaatctg tcccgtcatg cgcatatccg gcaaccggcc attaaccggc tgtggggatg
     9121 ggcgatgatt gcccagttcg catattatct ggccggcttt ccctggtatg aggggaatat
     9181 cctgtttgcc tttgcagtgg cggcacaggt gctgacatgg tgtgaaacgc gcagtgggtg
     9241 gcgtactgca gcggccattc tcctgatggc actgtggggg cctttgtccg gcaccagtta
     9301 cggcattgcc gggctgctga tgctggcagt cagtcaccgg ctgtaccggg cggaagacag
     9361 aacggaacgt ctggtgctgg ttgcatgcct gctggccgtg attccggcac ttaaccttgc
     9421 ctccagtgat gcggcggctg tagctggtct tgtgatgacg gtgctgaccg ttggtctggt
     9481 gtcgcgtgta ggaaaatcat taccccgttt ctggcccgga gattttttcc cgacgttcta
     9541 tggctgtcat ctggctgtgc tgggcattct ggcgttgtga ctggtatgat actgacgctg
     9601 attctgctta ttaataaaca acataacaca ggatatttat gaaaattacg gatcacaaac
     9661 tctctgaagg tattgccctg acatttcgtg ttcctgaagg aaatataaaa catcctttga
     9721 ttatcttatg ccatgggttt tgcggtatca ggaatgttct gctcccttgc tttgcgaatg
     9781 ccttcacaga agcaggattc gcaacgatta cttttgatta tcgaggattc ggtgagagtg
     9841 atggagaacg aggtcgtctg gttcctgcaa tgcagaccga agatattatt tcagttatca
     9901 attgggcaga aaagcaggaa tgtattgata atcagcgtat tgggttatgg ggaacatcgc
     9961 tgggaggggg gcatgtcttc agtgcagctg cgcaggacca acgcgtaaaa tgtattgtca
    10021 gccagctggc ttttgctgac ggtgaggttc tggttaccgg agagatgaat gaatcggaga
    10081 gagcgtcttt tctatccacg ctgaataaaa tggcagagaa gaagaaaaac accgggaaag
    10141 agatgtttgt gggggttaca cgggtactaa gtgatgatga atcgaaggta ttcttcgaga
    10201 aaatcaaagc ccggcatccc gaaatggata taaaaatacc tttccttacg gtcatggaaa
    10261 cgttgcaata taaaccggca gagagcgcag ccaaagtaca gtgcccagtt ttggttgtta
    10321 ttgccggcca ggatagcgtt aatccaccgg aacagggaag agcattatat gatgctgttg
    10381 cttctggtac taaggaatta tacgaggaag ctgatgcatg ccattatgat atttatgagg
    10441 gcgcgttttt tgaacgtgta gtagctgtgc agacacaatg gtttaaacaa tatctttaat
    10501 tgtcagtatt tatacgttaa aggtcgttga aattaacttc ctgttacgat gagttgtttc
    10561 aacggcctgc agggcatatt ttcacgaagg tcagagtaag catggcagag caaaaacgac
    10621 cggtactgac actgaagcgg aaaacggaag gagagacgcc tgtccgcagc cggaaaacca
    10681 tcatcaatgt caccacgcca ccaaaatgga aggtgaaaaa gcagaaactg gcggagaagg
    10741 ctgcccggga agcagagctg gcggcgaaga aagcacaggc cagacaggcg ctgtccattt
    10801 atctgacgct gccctcgctg gatgaggcgg tgaacactct gaaaccctgg tggccgggat
    10861 tgtttgacgg taacacgccc cggcttcttg cctgtggtat ccgggaagtt ttactggatg
    10921 aggtgtctca gcggaatatc ccgctctctc ataaaaaact gcgcagggca ctgaaggcca
    10981 tcacccgttc agaaagctat cttggcgcca tgaaagccgg tgcctgccgc tatgatacag
    11041 aaggctatgt gacagaacat atcacacagg aagaagaaca gtatgcgcag gcgcgcctgg
    11101 aaaaggtgcg cagacagaac cggatcaagg atgagctgcg ggcgatcctg gcagaataag
    11161 aaaagcgccc tctgtatgac agagggcaac agcgaaaaaa atcgggaaaa cagacatcca
    11221 gagttttgat ggagaaaaac atccaaccgt tcagggatgt ttcataatgt ttctgacata
    11281 atccgggtgt cgggtcaaga ggagggataa aaaatggctg tatccagtgg attatctgaa
    11341 cacagcagta tttatcgcta tcagcgcgag gggcgtcatg ccgtccgtca ggagattcgt
    11401 ggctttttca gggaagcggc agaggtctgg cagaatgcgg cccggctggc accctgtgaa
    11461 gcatggcggg attttgctct gaagagggca tctgcatgtc ggtgtaagtg ccgataaaaa
    11521 taacccccgt cgcggagctc cgggggatca gacttatatg gcagatcgtt attattatta
    11581 tagtggtgta agaaggagtt gccctgtctt tcaaacggac atcacagaat taacatattt
    11641 cacatcccgg gtaagccagc agggataaaa aatgtgatga atttcaagaa gcaggaggtg
    11701 acagtggcat gcataacagt agggttatcc ggtgaatctg acagacagcc gggctgtatt
    11761 cgcggcagga taaagaaaaa ccgggagatg actctcccgg tccgtcttat cgctaatccc
    11821 ctgagcaggg catgtatgac ttatctatga ccgttatatt cagcgccaca tccggtagcc
    11881 tgcgatataa cacagttatc ctgtcagcaa gagcaggtat gatgaataca cggagattaa
    11941 aaatataatc tccgtcacat gttacccacg gaaaccggta acgccttcac gggaaatatg
    12001 tgacagaaaa agcagcctcc gttactccag gtatcaggag gctgaataat gcatgagacg
    12061 ttgtttctta tgtgtctggt caggctggct gaccagcgtt tttagtaaca catagccata
    12121 tgtgtattaa gtcacctcat cttattttgt tgaattgatc acaactcaac ggtttatttt
    12181 ttgcgtgata cgtgagccat tattcatccc ggcatggaat tcatgcaatc acgaaaagta
    12241 atcagtgacg gagagtaaaa tgaagaaata tataccagtg gtattatttg ttttttcatg
    12301 tcaggtattt tctgctgata ttcatggacg ggtcgtgcgt gttctggatg gcgataccat
    12361 tgacgtcatg ttatctcagc atccggtgcg tgtccgtctt gttaatattg atgcacctga
    12421 aaagaaacag gaatatggac ggtggtctga aaaaataatg aaatctctgg ttgcgggaaa
    12481 aacggttact gtcacatatt ttcagcgaga tcattatggt cgtattctcg ggcaggttta
    12541 tgcaccagac ggaatgaatg ttaatcagtt tatggtccgt gcaggcgatg catgggttta
    12601 tgagcagtac aacaccgatc ctgttcttcc tgtcctgcag ggagaagtac gacagcagaa
    12661 acgtggtctg tgggcagaca gcgctcctgt tccgccctgg gtatggcgct cacggaaata
    12721 atgatatttc tgtggctatt gagttctcat tgaaatgcga tatttaaaag catgaagcat
    12781 attacattat taacttattt ctctgttaat gaaaggtgaa caaatgaact atcaggcagt
    12841 atcagaatta atcacatcgt ctaatcataa tgttcttata gtgggggaat cagcttctga
    12901 agttgatagc tttttaaata aactgaatat cactgattat aagtattatg atttcagcca
    12961 gatttacagt tgttctgaca gaacattaaa tgattatgct gttattttta tcaggaatgc
    13021 gctgaacgcc agtgagcata ttattatttt taattgtaca ggatggtctg atttgaataa
    13081 tgaatctgcc gtcatgcagt ttgccagagt ggcacgaaaa tcaggaaaac aactgatagt
    13141 cgcagtaaga gaacaggata tgaaaaaaat ggaagcagag tttggaagga tcataaaaat
    13201 ccactgacgc cttcgcgttg ctcagttgtc caaccccggg gacgggaaaa agcaagtttt
    13261 ccccgccccc ggcttttctg tagtgaaaag cgggaaatgg agggaatggt cagtaacagg
    13321 gaatgtggta ttatcgcggc gggtgtctga gcctttctgg ttcaggcaag acgcaggtac
    13381 cagaaatgcg aagaccccac ttgttaatcc attaactcgt gaggcctgca tgaagtacct
    13441 taacactact gattgtagcc tcttccttgc agagaggtca aagtttatga cgaaatatgc
    13501 ccttatcggg ttgctcgccg tgtgcgccac ggtgttgtgt ttttcactga tattcaggga
    13561 acggttatgt gagctgaata ttcacagggg aaatacagtg gtgcaggtaa ctctggccta
    13621 cgaagcacgg aagtaagctg ccgggcgggg agggaagtcc ccgctttccg gaagtgtgag
    13681 gtatttcagg ggcagacacc cgacatgcca gaaacagccg gtcccgcccg gggccggcac
    13741 acaggttaag gcatttccat cttttcagtc atttcattat caaaatcaca ttaaacggtc
    13801 gtaatcagac atgatttgtg cgccaacaca gatcatcgtc acaactttca agtcgctgat
    13861 ttcaaaaaac tgtagtatcc tctgcgaaac gatccctgtt tgagaattga ggaggcgaga
    13921 tgtcgcagac agaaaatgca gtgacttcct catcaggtac aaagcgagca tacagaaaag
    13981 gtaatcctct gacgcttgca gaacgtcagc aggcatccct ggcaagaaaa cgagccacac
    14041 ataaagaact cagagtgttt attcctgctg cgttaaaagc gcaattgcag gagatgtgtg
    14101 aagctgaggg tgttactcag gctgagatga ttgccgaact gataaagcag aagagtgctt
    14161 ttagctaact gaaatgtagc gacatcacat tcttgcctgt ttctggtgtg cgtgatagat
    14221 tgctgatcgt ttaaggaatt ttatggctgg ccacgccata aggtggcagg gaactggttc
    14281 tgatgtggat gtacaggagc cagaaaagca aaaaccccga taatctttct atgtttggcg
    14341 acgagaagat taccggggcc ccataaaacc gcatagccaa caattcagct atgcggggag
    14401 tatagttata tgcccggaaa agttcaagac ttctttctgt gctcactcct tctgcgcatt
    14461 gtaagtgcag gatggtgtga ctgatcttca acaaacgtat taccgccagg taaagaaccc
    14521 gaatccggtg tttacacccc gtaaaggtgc cggaacgctg aagttctgcg aaaaactgat
    14581 ggaaaaggcg gtgggcttct cctcccgttt tgatttcgcc attcatgtgg cgcatgcccg
    14641 ttcgcgtggt ctgcgtcggc gcatgccacc ggtactgcgt cgccgggcca ttgatgcgct
    14701 gctgcagggg ctctgttttc actatgatcc gctggccaac cgcgtccagt gctccatcac
    14761 cacgctggcc attgagtgcg gactggcgac agagtccggt gcaggaaaac tctccatcac
    14821 ccgtgccacc cgggccctga cgttcctgtc agagctggga ctgattacct accagacgga
    14881 atatgacccg cttatcgggt gctacattcc gaccgacatc acgttcacac cggctctgtt
    14941 tgctgccctt gatgtgtctg aggatgcagt ggcagctgcg cgccgcagtc gtgttgaatg
    15001 ggaaaacaga cagcgcaaaa agcaggggct ggataccctg ggtatggatg aactgatagc
    15061 gaaagcctgg cgttttgtgc gtgagcgttt ccgcagttac cagacagagc ttaagtcccg
    15121 tggaataaaa cgtgcccgtg cgcgtcgtga tgcgaacagg gaacgtcagg atattgtcac
    15181 cctggtgaaa cggcagctga cgcgtgaaat cgcggaagga cgtttcactg ccagtcgtga
    15241 ggcggtaaaa cgcgaagtgg agcgtcgtgt gaaggagcgc atgattctgt cacgtaaccg
    15301 taattacagc cggctggcca cagcttcccc ctgaaagtga cctcatcaga ataatccggc
    15361 acgcgccgga ggcattcgca cgcctgaagc ccgccagtgc acaaaaaatc agcaccacat
    15421 acaaaaaaca acctcatcat ccaccttctg gtgcatccgg tcccccctgt ttttgataca
    15481 aaacacgcct cacagacggg ggattttgct tatccacatt taactgcaag ggacttcccc
    15541 ataaggttac aaccgttcat gtcataaagc gccagccgtc agccttacag ggtgcaatgt
    15601 atcttttaaa cacctgttta tatctccttt aaactaccta attacattca tttaaaaaaa
    15661 aacctattca ctgcctgtcc tgtggacaga cagatatgca cctcccaccg caagcggcgg
    15721 gccccgatcg gagccaattt agttacaaca ttcaaacata gactccggaa aaaccccggt
    15781 tccgcgcaga actgaaacca caaagcccct cctcata
//
LOCUS       DAZGVQ010000118        15691 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000118 DAZGVQ010000000
VERSION     DAZGVQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.119"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            133..675
                     /gene="yfaZ"
                     /locus_tag="ACHLAG_003660"
     CDS             133..675
                     /gene="yfaZ"
                     /locus_tag="ACHLAG_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFP0656815.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            775..1977
                     /gene="yfaY"
                     /locus_tag="ACHLAG_003661"
     CDS             775..1977
                     /gene="yfaY"
                     /locus_tag="ACHLAG_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFP0656816.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELILTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            2041..2502
                     /locus_tag="ACHLAG_003662"
                     /pseudo
     CDS             2041..2502
                     /locus_tag="ACHLAG_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(2543..3445)
                     /gene="rpnE"
                     /locus_tag="ACHLAG_003663"
     CDS             complement(2543..3445)
                     /gene="rpnE"
                     /locus_tag="ACHLAG_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFP0656817.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRQLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLGRQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(3639..4829)
                     /gene="glpC"
                     /locus_tag="ACHLAG_003664"
     CDS             complement(3639..4829)
                     /gene="glpC"
                     /locus_tag="ACHLAG_003664"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFP0656818.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSTAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(4826..6085)
                     /gene="glpB"
                     /locus_tag="ACHLAG_003665"
     CDS             complement(4826..6085)
                     /gene="glpB"
                     /locus_tag="ACHLAG_003665"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFP0656819.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(6075..7703)
                     /gene="glpA"
                     /locus_tag="ACHLAG_003666"
     CDS             complement(6075..7703)
                     /gene="glpA"
                     /locus_tag="ACHLAG_003666"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFP0656820.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            7976..9334
                     /gene="glpT"
                     /locus_tag="ACHLAG_003667"
     CDS             7976..9334
                     /gene="glpT"
                     /locus_tag="ACHLAG_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFP0656821.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            9339..10415
                     /gene="glpQ"
                     /locus_tag="ACHLAG_003668"
     CDS             9339..10415
                     /gene="glpQ"
                     /locus_tag="ACHLAG_003668"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP0656822.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(10457..10696)
                     /locus_tag="ACHLAG_003669"
                     /pseudo
     CDS             complement(10457..10696)
                     /locus_tag="ACHLAG_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            10694..10798
                     /locus_tag="ACHLAG_003670"
                     /pseudo
     CDS             10694..10798
                     /locus_tag="ACHLAG_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10878..11528
                     /gene="inaA"
                     /locus_tag="ACHLAG_003671"
     CDS             10878..11528
                     /gene="inaA"
                     /locus_tag="ACHLAG_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFP0656823.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(11582..11836)
                     /gene="yfaE"
                     /locus_tag="ACHLAG_003672"
     CDS             complement(11582..11836)
                     /gene="yfaE"
                     /locus_tag="ACHLAG_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFP0656824.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(11836..12966)
                     /gene="nrdB"
                     /locus_tag="ACHLAG_003673"
     CDS             complement(11836..12966)
                     /gene="nrdB"
                     /locus_tag="ACHLAG_003673"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP0656825.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(13113..15398)
                     /gene="nrdA"
                     /locus_tag="ACHLAG_003674"
     CDS             complement(13113..15398)
                     /gene="nrdA"
                     /locus_tag="ACHLAG_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP0656826.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            15526..15669
                     /gene="ypaB"
                     /locus_tag="ACHLAG_003675"
     CDS             15526..15669
                     /gene="ypaB"
                     /locus_tag="ACHLAG_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFP0656827.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
BASE COUNT         3647 a         3894 c         4274 g         3876 t
ORIGIN      
        1 ttaattattt aagaagtatt tagttacaat aagtgactag atcccttttt tcgctcattg
       61 aatcctctgt gccaggcagt acaatcccgc agctttggga attttgatta attgcttgtg
      121 ggaaagggga taatgaaaaa aattgcgcta gcaggtctgg ccggaatgct gttggtttct
      181 gcatcggtca atgcaatgag catcagcggc caggcgggta aagaatacac caatattggt
      241 gtcggttttg ggactgaatc gacgggcctg gctttaagcg gtaactggac acataacgac
      301 gacgacggtg acgtcgcggg cgtggggctg gggttgaatc tgcctctcgg gccgttaatg
      361 gcgaccgttg gcggaaaagg cgtttacacc aacccgaatt acggcgatga aggttatgcc
      421 gcagcggtag gaggtggttt gcagtggaaa attggcaaca gcttccgttt gtttggcgag
      481 tattactact ctccggattc gctctccagc ggtattcaaa gttatgagga agcgaatgct
      541 ggcgcgcgtt acaccattat gcgtccagtc agtattgagg cgggttatcg ctacctgaat
      601 ctgtcgggta aagacggtaa ccgcgacaac gccgtggctg acggcccgta tgttggggtt
      661 aacgccagtt tctgatccct tccggcgcgg cattctgtcg cgccattcag tttcttacta
      721 cgccttatct ccctttgcgt tatagtgatt cgataacaaa agcaggagat aaccatgtta
      781 aaagtggaaa tgctatccac cggggatgaa gtgttacacg ggcaaatcgt tgacactaac
      841 gctgcctggc tggccgattt tttctttcat caggggctgc cattatctcg ccgcaatacg
      901 gtgggggata accttgatga cttagtcacc attcttcgcg aacgtagtca gcacgccgat
      961 gtgctgatcg ttaacggcgg gctgggaccg accagcgatg atttaagcgc actcgccgct
     1021 gcgacagcaa aaggtgaagg cctggtgctg catgaagcct ggctcaaaga gatggaacgc
     1081 tatttccacg aacgtggacg agtaatggca ccgagcaacc gtaaacaagc ggagctgcct
     1141 gccagtgctg aatttatcaa taacccggta ggcaccgcct gtggttttgc cgtgcagctt
     1201 aatcgttgcc tgatgttctt tactcccggc gtaccgtcag aatttaaggt gatggtcgag
     1261 cacgaaatcc tgccgcgtct gcgcgagcgt ttttctttac cacagccgcc ggtttgtctg
     1321 cgtttaacta cctttggtcg ttcggaaagc gatctggcac aaagcctgga cactctacaa
     1381 ctgccgccgg gcgtaacaat gggctatcgc tcttcaatgc cgattatcga actgatactc
     1441 accggaccgg caagcgagca acaggcgatg gaaaaactgt ggctggacgt taaacgtgtt
     1501 gccggacaga gcgtgatttt cgaaggcacc gaaggactgc ccgcgcagat cagtcgcgaa
     1561 ttgcaaaacc gccagttcag cctgacgttg agcgagcaat tcaccggtgg tttattggct
     1621 ttgcaacttt ctcgcgcagg tgctccattg ctggcgtgtg aagtggttcc ttcacaggaa
     1681 gaaaccctgg cgcaaactgc gcactggatt acagaacggc gggccaacca ttttgccggg
     1741 ctggcactgg ctgtttcggg tttcgagaac gaacatctca actttgcgct agccacacca
     1801 gacggcactt tcgctctgcg tgtgcgtttc agcactacgc gctacagcct ggctatccgt
     1861 caggaagtgt gcgcaatgat ggcactgaat atgctgcgcc gttggttaaa cggccaggat
     1921 atcgccagtg agcatggctg gattgaggtt attgagtcca tgaccttatc tgtctgattc
     1981 cgccactctg tgccggttta ctgcaccggc acattccccc tccggcaaaa cttgtccaac
     2041 cctccctacg gtgagcgtgg tgttggggcc agtgtggcac gggctgcgcg ctggggacgc
     2101 attgagaatt acatggcgca agttaacgat tcactttgtc tgttggtgca ggtggaaagt
     2161 aaaacggcac tggataacct ggacgagatc ctcgacgtcg aagggattga tggcgtgttt
     2221 attggaccag cggatctttc tgcgtcgttg ggctacccgg ataacgccgg gcaaccggaa
     2281 gtgcagcgaa ttattgaaac cagcattcgg cggatccgtg ctgcgggtaa agcggctggt
     2341 tttctggctg tggctcctga tatggcgcag caatgcctgg cgtggggagc gaactttgtc
     2401 gctgttggcg ttgacacgat gctctacagc gatgccctgg atcaacgact ggcgatgttt
     2461 aaatcaggca aaaatgggcc acgcataaaa ggtagttatt gatatcaaag gcccatgggg
     2521 atcggctgtg ggcctgtgtt aattagtggt tattcgctgc cagatcggct tcgcttagct
     2581 gggtggccgc gagcacctgg tcacgatcaa tgccctgttc cagcatgcgc aaagcaattt
     2641 taatggcttg ttcatgcata ccttcctgcc agccaatttg atgcccttcc tgccgtagcc
     2701 gttcagcaat agtcattaat ctctcctttt gtagtggtga acgttcggca atctcctcaa
     2761 taaaacgggt gaaacgggag gtatcgccgc attgcagcag ataattaaac agtgtttgta
     2821 gctggctgtc attagtgaag cctctaacca aaagcgtggt gatcctgtca accatgccga
     2881 ttaaatcgcg gtcgcgaata tgcttttgaa tcagttccag cagagctata cgccgatgtt
     2941 gcatgatctc atcgtcaggt acgatggtga tatccaccaa cggaaaagct tcggtgtaca
     3001 actgccggcc aagttgcgga tcgtcaaact catccagcca gttaagtgag tacgggtagg
     3061 gcgatgtttc gccatgataa aacagcaatg gcaccaccag cggaactctg tcatagcctt
     3121 tatccagctg acgctgcatg gcggcagtgg catagcgcat tagccgaaaa gccatattct
     3181 tttctgcaga gctttgatgt tcaatcacgc agtagatata gccgtcaccg tcgctggttt
     3241 ccacggacca caaaacatcc gagtagtaag cgcgtaaact tttttcaata aaactagtgg
     3301 gttccaggcg taaggtttgc aggttgcaaa gcttgcgcag tggttctggt aaatgtattt
     3361 cgagaaaatc ccgtgcggtt tcgggtgtga acataaaggt tttaaacacc gcatcatgcg
     3421 gggaggatgt tgttgattcg gtcatggttg tccgtcacct gatatttatg atgacgcgac
     3481 attacgtcta tcccgggcct ttgacatctg tcagtttcag cctttgcgag acattttcag
     3541 aggaattttc aaagcgttgc aacgcataca tggtgcgtgg aatcgcgctc tcaaaaatgc
     3601 ccgataagca atgtgcttac cgggcatcaa aaggtcggtt aagccagcgc ctgggccagt
     3661 agcgtaatcg gatgttcgca gcgaaggctc gtggacatct caatctgcca tttgcaggtt
     3721 tcgcagtcgg tgatgaccag atccgcgccg ctctcttcta tctggcggaa cagtggcgca
     3781 ccgatggctt gtgaggtggg gtagttctct tttttgaaac cgtaagtacc cgcaataccg
     3841 cagcactggg aatccagcac cgttaactca agccccggga ttttacgcaa cagctccagg
     3901 gtgtagagcg tccagcccat tttttccata tggcacggag tgtgataaac cactttcagc
     3961 ggcagcggtt tcagcggtaa cgttttgcct tcgtccagct tgcgccacag ccagcgggtt
     4021 gccagttcga tatgatcgcg caagcctttg ttgtcgacat tcagcacttc cgggtattcg
     4081 tcgcgcaggg caaatgtaca ggttgaggag gtggcaatca ccggaatgcc ttttactccc
     4141 acagcttcgc ggatcgactc tacattcgta attgcctgtt tgcgtgcttt atcggtaaag
     4201 ccgttggcga ttagcggtac gccgcagcat ttttctttgc tgagcagttg tacaccggta
     4261 cccattgcgt tgagcacttt aattaaatct ttacctaact gcggatggtt gtagttaacg
     4321 aagcagccgt gaaagaaagc gacctggtct ttatattgtg cttgctgagc cgctgtgctg
     4381 cgataccagc gacggaacgt gccgaaggag tatttcggta gcgtgcggcg atgatcgatt
     4441 tttaacgccg catcaagcag ctgccgcacc ggtttcagcg aggtagcggt gttgacgatt
     4501 ggtgcgaacg gcgtggaaac gctacccatc aggtcggtat gactcaacac aaaattacgc
     4561 agcgacgggc gcgtggtgtc atattttgcc cgcgcgcgct ggataatatc gccaatcttc
     4621 acatcggacg ggcaggcgac ttcacaacgt ttgcagttga tgcaatattt cagcgcctcg
     4681 tcatacagtg cgccatcttt caaacgcaga cgctcgccat ccggcccggc ttgttttggc
     4741 cctggataac cgggattcac ccggctcacc gggcaggcgg tggtgcagac ggtgcactta
     4801 atgcagtttt cgaagctggt gtcgttcatt gttggcctcc tgcgcgttgg gcaatctgtt
     4861 gagcggcatg taaagcactg acggcacaaa caccgccgcc gcatccctgg gcgatgggat
     4921 caaatccgcc cagcaccgaa ccgatggcaa acaggttctc aatggtttgc cctgcctgcg
     4981 acgggcgtag cgtctcatca gtggttacac cgaactgctg ccacggttgc ggcgcaaaaa
     5041 aatcgccctt ataccattca ccccgcgtgg cggtttgtag cacatcaagg ccgagaatcg
     5101 gctcgcgaat gccgttacgt tccgctacca gtccgccact aaagaaactg ccgctggcga
     5161 gaaccgcgaa acgtggacgt agcggaatat cggcgtgatt gcgggtccag atttcattca
     5221 ctacgccatt tttacaggtc acttttttca cttcatcgcc cggcatccac acgccaccct
     5281 ggcgcacaaa ctggcgctgt aactggtttt gcagacgaat gcccagcacg gaaggcggca
     5341 gcgttggcaa aagcatcagt gaacaaggta gtttttcatt caaccaacgc cacagtttgt
     5401 catcggccag gccgaagcag gcgggcatca ggatcatttc gcaggtattg gcgacaggaa
     5461 taagcgcatc aagtaacagc ggccagtttt cttcattatc aaggaaacgg gcgatattca
     5521 ccgcgcgaaa ttcggtggcg ttattgcgca ggacatccag ttccggcagc tctatttctg
     5581 cggtttcaac cgaaagatcc agttcgcgca acgaagccgc cgcaagatgc gcctggaaat
     5641 ccatcagccc gcttatcccc actacacata tcttcttcgc gggtagcggc cagacgggaa
     5701 cttctggcga acttaaccag gttgcacgca aagtgccgag cggggtaacg cgctgatggg
     5761 ccagttccac gctgccctgc aattgcgcac cgctttcagc gatcaatgcc tgcgcctggc
     5821 aagcgagatc gagcacgcgt tgtggttcga gaagactgta aggatgggct ggtgcctgct
     5881 gacgcaaaga ttccagtcca ctgtgaatgt ctgtcaccgg ttgaccatca ggcagatggc
     5941 tcagcaaatc cagcgaaccg gatgagaaat gcagcgcgct ttgaccacgc gtgacaatgg
     6001 cacagcgcag gccgtgtttt tgcagttgca ggccacagag taatccggcg aggccgccgc
     6061 ccataatgac agtatcaaag cgcatctttc tgctccttct ccagaccaca caatccctga
     6121 taaacccagc gggtaaattc gctttcgcgc agtgcatctc cccaggcgat gggttgcacg
     6181 cctttccagc gttcgttaag gaaggtggaa agttgctcga tagattgcgc ggacgtcgtg
     6241 acgttaaaac gttgcagcag tccggcagcg cggcaggcgc agagttcgcc ctggcaggta
     6301 cccatcccca cacgggtacg acggcgtaaa tccagcaggc tattaacgtt taaattttct
     6361 accgcgtact gaacttcacc cgcagttacc gcttcgcact cacataccag gctacgatgc
     6421 agacggcctt cgctcagcca ggcaggcgtg cgatcgccat gacgataaac cgcagaaccg
     6481 cgcagcgggg caggcaggga gatgacttta cgcaaggtaa cttcagcggg ttcttgtgaa
     6541 ccaggcagtg ccagatcggc agtcgtacag gggcgcgtgt tgcccagttt gcggcatacc
     6601 gcgtcggtag cccattcagc catcagccga taggtcatca gtttgccacc ggtgatggtg
     6661 ataaatccgt ccagaccatc gcgttcagca tggtcgagca gcacgatgcc acggctgacg
     6721 ttacgtccgc tcgggtcgtc atcgctggca accagcgggc gcacgccaga ataggcccgc
     6781 aaaatgcgcg ttttcgccat cacgggggcc agtttttccc cttcacgcag cagaatatca
     6841 acctcttctg ccgtcactcg attatcgtca atctcgttgt aatcaatacg taaagaggtg
     6901 gtgccaatca gcgaaatggt atcgccaggc accagaatat cggcgtcgga aggtttacgg
     6961 cagcggttga tcacatgctg gttaatgcgg tgatccatga tcagcagcga tcctttcgcc
     7021 gggaacatgc gaatgcgcag atcggcatat tctgcaatgt gttgccccca gatcccagcg
     7081 gcattaacca cgacaggtgc atgaagggcc tgagtttcgc cggtgagatg attacgtaca
     7141 cgaacaccgc acaccgtcgc gccttcacga atcagccccg tgacttcatg agcggtaagg
     7201 ataacggcac cgtgttcttt ggcatccagc atgtttgctg cggtcagacg aaatggatca
     7261 acggtgccat ccggaacttt caccgcgcca atcagtgccg ggttaacggc aggttcgata
     7321 atgcgcgctt gctgcgggtc tatagcttct gcgctgatcc ctgcttcttc gcaggcgcga
     7381 ataaaagtgg cctggaatga gaggtcatct tccggcaggg tgataaacag gccgttggtt
     7441 ggttcaacgc agtgacgtgc aatgcgtttc aggatctggt tttcactaat gcattcgcgg
     7501 gccgattccg catcggttac cgcatagcgc gcaccgctgt gcagcaggcc gtggttacgc
     7561 ccggtggcac cggttgcgat gtcgtggcgc tcaaccaaaa tcacgcgcag cccgcgcagg
     7621 gcacagtcgc gggcaatccc ggctcccgtt gcgccgccac caatgataat cacgtcactt
     7681 gattgcgagt cgcgagtttt cattgttatc cctctgaagt tcgtttttta ccatttagcc
     7741 atagtaaaaa catgaattgt ttgatttcgc gcatattcgc tcataattcg aaagtgaaac
     7801 gtgatttcat gcgtcattct gaacattttg taaatcttat ttaataatgt gtgcggcaat
     7861 tcacatttaa tttctgaatg ttttcttaac atcgcggcaa ctaaagaaac ggcaggttct
     7921 cccactgaat cagtctgtta atcataaata agaccacggg ccacggaggc tttcaatgtt
     7981 gagtattttt aaaccagcgc cacacaaagc gcgcttacct gctgcggaga tcgatccgac
     8041 ttatcgtcga ttgcgctggc aaattttcct ggggatattc tttggctatg cggcttacta
     8101 tttggttcgt aagaactttg cgcttgctat gccttatctg gtcgagcagg gattctctcg
     8161 cggtgattta ggtttcgcgc tttcggggat ctcgatcgct tatggatttt cgaaattcat
     8221 aatgggttcg gtatcggatc gctcgaatcc gcgcgttttc ctgcccgcag gtttgattct
     8281 ggcggcggca gtgatgttgt ttatgggctt tgtgccatgg gcgacgtcga gcattgcggt
     8341 gatgtttgta ctgttgttcc tctgcggttg gttccagggg atggggtggc cgccgtgtgg
     8401 tcgtactatg gtgcactggt ggtcgcagaa agaacgtggc ggcattgtgt cagtgtggaa
     8461 ctgtgcgcac aacgtcggtg gtggtattcc gccgctgctg ttcctgctgg ggatggcctg
     8521 gttcaatgac tggcatgcgg cgctctatat gcctgctttc tgcgccattc tggtggcatt
     8581 attcgccttt gcgatgatgc gcgatacccc gcaatcctgt ggcttgccgc cgatcgaaga
     8641 gtacaaaaat gattatccgg acgactataa cgaaaaagcg gaacaggagc tgacggcgaa
     8701 gcaaatcttc atgcagtacg tactgccgaa caaactgctg tggtatatcg ccatcgccaa
     8761 cgtgttcgtt tatctgctgc gttacggcat cctcgactgg tcaccgactt acctgaaaga
     8821 ggttaaacat ttcgcgctgg ataaatcttc ctgggcctac ttcttctatg agtatgcggg
     8881 tattccgggc accctgctgt gcggctggat gtcggataaa gtcttccgtg gcaaccgtgg
     8941 ggcaaccggc gttttcttta tgacactggt gaccatcgcg actatcgttt actggatgaa
     9001 cccggcaggt aacccaaccg tcgatatgat ttgtatgatt gttatcggct tcctgatcta
     9061 cggtcctgtg atgctgatcg gtctgcatgc gctggaactg gcaccgaaaa aagcggcagg
     9121 tacggcagcg ggctttaccg ggctgtttgg ttacctgggc ggttcggtgg cggcgagcgc
     9181 gattgttggc tacaccgtgg acttcttcgg ctgggatggc ggctttatgg taatgattgg
     9241 cggcagcatt ctggcggtta tcttgttgat tgttgtgatg attggcgaaa aacgtcgcca
     9301 tgaacaatta ctgcaaaaac gcaacggagg ctaatggcat gaaattgaag ctgaaaaacc
     9361 ttagcatggc gatcatgatg agcactatag tcatgggaag cagtgcaatg gcggcggaca
     9421 gcaacgaaaa aatagtcatc gcccatcgcg gtgccagtgg atatttgccg gagcatacgc
     9481 tgccagcaaa agcgatggcg tatgcgcagg gagcggatta tctggaacag gatttggtga
     9541 tgaccaaaga cgaccatctg gttgttctgc atgaccatta tctcgatcgt gttactgatg
     9601 ttgccgatcg tttcccggat cgggcgcgca aagacggtcg ttactacgcg atagatttca
     9661 cgctggatga aattaagtcg ctgaaattta ccgaaggttt cgatattgaa aacggtaaaa
     9721 aagtacagac ttatccgggg cgtttcccaa tgggtaagtc cgacttccgg gtgcacacct
     9781 ttgaagaaga gattgaattt gttcaggggt taaatcactc taccgggaaa aatatcggta
     9841 tctatccaga aattaaagcg ccgtggttcc atcatcagga agggaaggat attgcggcaa
     9901 aaacgctgga agtgctgaag aaatatggtt acaccggtaa ggacgacaaa gtttatttgc
     9961 aatgttttga tgctgatgag ctgaagcgta ttaagaatga gctggaaccc aaaatgggca
    10021 tggatctcaa tctggtacag ctgattgcct ataccgactg gaatgaaacg cagcagaaac
    10081 agccggatgg aagctgggtt aattacaact acgactggat gtttaagccg ggtgctatga
    10141 aacaggtggc ggaatatgcc gacggtatcg ggccggatta ccatatgttg attgaggaaa
    10201 catcgcagcc gggtaatatc aaactcactg gcatggtgca agatgctcag cagaataaac
    10261 tggtagtgca tccttatacc gtgcggtcag ataaactgcc tgaatacact actgatgtga
    10321 atcagttata tgatgctctg tataacaaag cgggtgtaaa tgggctgttt actgatttcc
    10381 ctgataaggc agtaaaattc cttaataaag agtaatgcta ctgcaggatg gcgttcatgc
    10441 gccatcctgt ctcacatcaa atttttccat tcaccacgag ttgttccgta cacgtttgta
    10501 gtaaaaacag tggactgcct tctaccggct tttctttagc caacaacgaa atctgtcgat
    10561 agaaggttgg ttcgtgagga acggaacaca attcacctgc aatgcttttc agcgttaact
    10621 ctggttgtaa ggcgatgcct aatccctgtc gaataaactt catcgctgta tccgactgat
    10681 taaattcaca ttttatctac tgggcgcact attagtggta gtggctggtg gaatctatgc
    10741 gctgcaaatg aagggtaaaa gcggtgtcac gctagtagtg gcaaaagaaa ttcattaata
    10801 cgacacgttt cattaagatt ttcctcaggt aaccacctat agtcattgtc gttaataaaa
    10861 atgacaggcg atagggtatg gcagtttcag caaagtacga cgaatttaat cactggtggg
    10921 caacagaggg cgactgggtt gaagagccta actatcgccg taatggtatg agcggggtgc
    10981 aatgtgtcga gcgcaacggc aaaaagctgt atgtaaagcg catgacgcat catctgtttc
    11041 attccgtacg ttatccgttc ggccgaccaa cgattgtccg tgaggttgcg gtaattaaag
    11101 aactggaacg ggccggtgtc atcgtgccga agatcgtttt tggtgaagcg gtgaaaattg
    11161 agggtgaatg gcgagcgttg ctggtgactg aagatatggc ggggttcatc agcattgctg
    11221 actggtatgc ccagcatgca gtatcgcctt attctgacga agtacggcaa gccatgttga
    11281 aagcagtggc gctggcgttt aagaaaatgc atagcattaa tcgtcagcat ggctgttgtt
    11341 atgttcgcca tatctatgtg aaaacagaag gtaatgcaga agctggtttt cttgacctgg
    11401 agaaaagtcg tcgccgcttg cgtcgcgata aagcgattaa tcatgacttc cgccagctgg
    11461 aaaaatatct ggagccaatc cccaaagcgg actgggagca ggtgaaagcg tattactacg
    11521 ccatgtaatc gttgtaggcc agataagacg cataagcgtt gtatctggca ctacaaccaa
    11581 ttcacatctc gatttcaata tcgccttttg cccggcaaca acagggcaaa atttcccccg
    11641 gctgaataaa ggctaacggt tcggcaatcc agtcaacctg acctgcaacc agacgggtgc
    11701 gacaggagcc gcagtaacct tcgcgacact gatactcaac cgccacattg tgggattcca
    11761 gcgccgccag aagggaaggg tgttcatcct ggcacagcag ttgtgtgcca gtgatgcgca
    11821 gggtaacgcg ggccatcaga gttggaagtt actcaaatcg tcggtgtcca cttccgcgtc
    11881 aatctgcccg accagataag aactgacttc cacttcctgc ggagcaacct gcacgttatc
    11941 agacaccagc caggtgttga tccatgggat cgggttggag cgcgtctgga acggcagatc
    12001 caaaccgact gcctgcatac ggatattggt gatgtattca acgtactggc agagaatatc
    12061 tttattcaga ccaatcatcg aaccgtcacg gaacagataa tccgcccagt ctttctcctg
    12121 ttgagctgcc tgaacaaaca ggtcatagca ctcctgctta cactcttcgg caatttccgc
    12181 catctcagga tcgtccgcgc cgctgcgcag cagattcagc atatgctggg tgccggtcag
    12241 gtgcagggct tcgtcgcggg caatcaggcg aataattttg gcgttgcctt ccatcaattc
    12301 gcgttctgca aatgcgaagg aacaagcaaa gctgacgtag aaacgaatcg cttccagcgc
    12361 gttaacgctc atcaggcaga gatacagttt tttcttcagc tcgcgcaggc taacggtcac
    12421 agttttaccg ttaacggtgt gggtaccttc gcccagcaga tgccagtagc tggtcatttc
    12481 gatcagctca tcgtaatagc tggagatccc ttccgcacgt ttctggatct gctcgttggt
    12541 gacgatatcg tcaaacacaa cagacggatc gttaacgata ttacgaatga tatgagtata
    12601 ggaacgggaa tgaatcgttt ctgagaacgc ccaggtttcg acccaggtct ccagttccgg
    12661 gatcgagatc agcggcagca gtgccacgtt cgggctacgg ccctgaatgg aatccagcag
    12721 cgtctgatat ttcaggttgc tgataaagat gtgtttttcg tgttcaggca gtgcctggta
    12781 atcgatacgg tcgcgagaaa cgtcaacttc ttccggacgc cagaagaaag agagctgctt
    12841 ttcgatcagc ttttcgaaga tgtcatattt ttgctgatcg tagcgagcca cgttgaccgg
    12901 ctgaccaaag aacatcggtt ctttgagctg atcatttttc gtctgtgaaa aggtggtata
    12961 tgccatgagt gtgtcctgtt gggagtttaa tgccggataa ggcattttac gccgcatcct
    13021 gtgcctgatg cgacgctgac gcgtcttatc aggcctacaa atccgagccg taggccggat
    13081 aaggcgttca cgctgcatcc ggcatctcaa tatcagatct tacatgcgcc gctttcgcag
    13141 ccatcgtcct ggattgacgg taccagatcg tcctgcgcgt cttcagcgcc gtcacgggtg
    13201 ttctgataat acagcgtttt gaccccgaat ttgtaggcgg tgagcaggtc tttcagcaac
    13261 tgctgcatcg gcacttttcc tgacgggaaa cgtgacggat cgtagttggt gttagcagag
    13321 atcgactgat cgataaattt ctgcatgata cctaccagtt gcaggtagcc atcgttaccc
    13381 ggcatttccc acagcagctc ataggcgtcg tgcaggtgct cgtagtccgg caccacctgg
    13441 cgcaaaatac cgtctttcga cgctttgatg ctgacgtaac cgcgcggcgg ttcaataccg
    13501 ttagtggcgt tagagatctg tgaagaagtc tctgacggca tcagggcaga aagcgtggag
    13561 ttacgcaggc cgtgcgtttt gattgattca cgcaatgctt cccagtcgta atgaagcggc
    13621 tcattagcga tggtatccag atctttctta taggtatcga tcggcaggat ccctttcgcg
    13681 taagtggttt cgttaaacca cgggcacgcg ccttgctctt ttgccagctc gttagacgct
    13741 ttcagcaggt aatactgaat cgcttcgaag gttttatgcg tcaggttgtt ggcgctgccg
    13801 tcggagtaac gcacaccgtg cttcgccagg tagtaagcga agttgatcac accaataccc
    13861 agcgtacgac gacccatcgc tccacgtttg gcggccggga tcgggtaatc ctgataatcc
    13921 agcagcgcgt caagtgcacg aaccgccaga attgccagct cttccagttc atccaggcta
    13981 ttaattgcgc ccaggttgaa agcagacagc gtacacagcg cgatttcacc gttctcgtcg
    14041 ttgacgtcgt tcagcggttt ggtcggcagg gctatctcca ggcacaggtt agactgacgc
    14101 actggcgcga tggccggatc aaacgggcta tgggtattgc agtggtcaac gttctgaata
    14161 tagatacgac cggtagacgc acgttcctgc atcatcagcg agaacagctc aacggctttc
    14221 acacgctgct tgcggatgct gtcgtctttc tcatatttgg tatacaggcg ttcaaactct
    14281 tcctgatcgg cgaagaatgc gtcgtacagc cccggtacgt cggacgggct gaacagggtg
    14341 atatcttcac ctttcagcag gcgggtatac atcagtttgt tgatctgtac cccgtagtcc
    14401 atatggcgca cgcggttgcc ttccacacca cggttgtttt tcaacaccag caggctttcc
    14461 acttccagat gccacatcgg gtagaacagc gttgccgcac cgccgcgcac gccgccctga
    14521 gagcaggatt tcaccgctgt ctggaaatgt ttgtagaacg gaatacagcc ggtatggaac
    14581 gcttcaccac cgcgaatcgg gctacccagc gcacgaatac gcccggcgtt gatgccgatc
    14641 ccggcacgct gggaaacgta tttcacaatc gcgctggagg tagcgttgat ggaatccaga
    14701 ctgtcaccgc actcgatcag tacgcaggag ctgaactgac gagtcggggt acgcacgccg
    14761 gacatgattg gcgtcggcag cgaaatttta aatgtggaaa ccgcgtcgta aaaacgcttc
    14821 acatattgca ggcgcgtttc acgcgggtag ttcgagaaca ggcacgcggc aaccagaata
    14881 taaaggaact gggcgctttc gtagatttcg ccggtcacgc ggttctgtac cagatatttg
    14941 ccttccagct gcttaacggc agcataagag aaggtcatat cacggtcgtg atcgataaac
    15001 gtgtccatct gcttgaactc ttcttccgtg tagtcttcca gcagatgatt atcgtatttg
    15061 cccatctcga ccattttcac cacgtggtcg tacagcgcag gcggctcaaa ctggccgtag
    15121 gcttttttac gcaggtggaa gatcgccagg cgcgcggcga gatactgata atccggcgca
    15181 tcacgggaga tcaggtctgc ggccgccttg ataatggttt cgtggatgtc agaggtcttg
    15241 ataccgtcat aaaactgaat gtgggagcgc agctcgacct gggaaatcga aacgttatgc
    15301 agtccttctg ccgcccaatc cagaacgcga tggattttgt cgagattgat gcgctctgtg
    15361 ctaccgtcgc gctttgtcac cagcagattc tgattcatgt atgtcgtacc tgtttttgga
    15421 attctttccg caatagtttc atgcagacta cctgtagtaa gggaagctgc ttagaacact
    15481 atatataggg ggtatgtttg ggattcactg caagatagtg tgaaaatggc cctcttgcaa
    15541 gtgcataact ttgtggataa ctcaggaagg aaaaagtggc tttcgcgcac cttaggtcag
    15601 acaaggtgtc cgggaaagtc aatgggaaga gaaaaatttg ttaaaaataa ctgttcggcg
    15661 atttcttgaa cggcagaata ggttggataa a
//
LOCUS       DAZGVQ010000119        15388 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000119 DAZGVQ010000000
VERSION     DAZGVQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.120"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(137..1048)
                     /gene="gsiD"
                     /locus_tag="ACHLAG_003676"
     CDS             complement(137..1048)
                     /gene="gsiD"
                     /locus_tag="ACHLAG_003676"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFP0656828.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1051..1971)
                     /gene="gsiC"
                     /locus_tag="ACHLAG_003677"
     CDS             complement(1051..1971)
                     /gene="gsiC"
                     /locus_tag="ACHLAG_003677"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFP0656829.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(1989..3527)
                     /gene="gsiB"
                     /locus_tag="ACHLAG_003678"
     CDS             complement(1989..3527)
                     /gene="gsiB"
                     /locus_tag="ACHLAG_003678"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFP0656830.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3547..5418)
                     /gene="gsiA"
                     /locus_tag="ACHLAG_003679"
     CDS             complement(3547..5418)
                     /gene="gsiA"
                     /locus_tag="ACHLAG_003679"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFP0656831.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5405..6369)
                     /gene="iaaA"
                     /locus_tag="ACHLAG_003680"
                     /pseudo
     CDS             complement(5405..6369)
                     /gene="iaaA"
                     /locus_tag="ACHLAG_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
     gene            6573..7808
                     /gene="moeA"
                     /locus_tag="ACHLAG_003681"
     CDS             6573..7808
                     /gene="moeA"
                     /locus_tag="ACHLAG_003681"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFP0656832.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGNGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLATLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            7808..8557
                     /gene="moeB"
                     /locus_tag="ACHLAG_003682"
     CDS             7808..8557
                     /gene="moeB"
                     /locus_tag="ACHLAG_003682"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFP0656833.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARYALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8736..9398)
                     /gene="fsa"
                     /locus_tag="ACHLAG_003683"
     CDS             complement(8736..9398)
                     /gene="fsa"
                     /locus_tag="ACHLAG_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP0656834.1"
                     /translation="MELYLDTSYVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            9529..10428
                     /gene="ybiY"
                     /locus_tag="ACHLAG_003684"
     CDS             9529..10428
                     /gene="ybiY"
                     /locus_tag="ACHLAG_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFP0656835.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10434..12866
                     /gene="ybiW"
                     /locus_tag="ACHLAG_003685"
     CDS             10434..12866
                     /gene="ybiW"
                     /locus_tag="ACHLAG_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFP0656836.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13012..13827
                     /gene="ybiV"
                     /locus_tag="ACHLAG_003686"
     CDS             13012..13827
                     /gene="ybiV"
                     /locus_tag="ACHLAG_003686"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFP0656837.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPHFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            13979..15244
                     /gene="ybiU"
                     /locus_tag="ACHLAG_003687"
     CDS             13979..15244
                     /gene="ybiU"
                     /locus_tag="ACHLAG_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFP0656838.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         3710 a         4258 c         3933 g         3487 t
ORIGIN      
        1 ctgcaaaaat agtcccttgc tgcggctttt taaccaaatt acccgattct acaaaatcag
       61 cagaaagtta caacacttgc ttagggcaat aattgcctgt gaggtgatta ccctttcaag
      121 caatattcaa acgtaactat cctttaattt tcggatccag cgcatcgcgt aaaccatcgc
      181 ccaacaaatt gaacgccagt acggtcagaa aaatagccag ggccggaaaa acagcgacat
      241 gcggcgcgat aaccatatcc gctcgtgcct cattgagcat tgctccccac tctggtgtcg
      301 gcggctgcgc accgaggccg agaaatgaca ggctggcggc agagataatc gaggtgccaa
      361 tgcgcatggt gaaaaacacc acgatagacg agacggtccc aggcaagata tgacgcaata
      421 aaatggtcat atcgctggca ccaatactgc gtgctgactc aataaaggtt tgctgtttca
      481 acaccagcgt gttgccgcgc accaggcggg caaacgcggg gatggaaaaa atggcgactg
      541 cgataatcac gttagcaatg ccgcttccca acaccgcaac aacagcgatc gccagtaaaa
      601 tacccgggaa ggcaaacagc acatcgcaaa tgcgcatgat cagccgatcc caccagcctt
      661 cataatatcc agccagcaag cccagcaacg tcccgatcgc cgcaccgata aacacggcaa
      721 acacgcccgc cgccagcgag atttgcgcac caaccaggac acggctgaaa atgtcgcgcc
      781 ccagtgaatc gacgccaaac cagtgctgca aagaaggtcc gttattcaga ttgtcataat
      841 caaaataatt ttcggcgtca tagggagcga tccagcgtgc aaaaatggcc accacaatca
      901 ataaaataac gaataatgcg gcggtcatcg ccatatgctg acggcgaaat cgtcgccaga
      961 attcatgcca cggtgtacgc acctggtcag gtttgaccag tggcatggcg tttaacaccg
     1021 cctgacgtcg ccagttaaat agtcgcatcc ttacttgtac ctgatagccg ggttaatggc
     1081 ggcgtaaagc acatccacca ctaagttgat aagaataaat tccagcgaga aaagcagaat
     1141 ttccgcctga atcaccgggt aatcgcgcat ttctacggag tcaacgagta agcgtccaag
     1201 tccgggccag ttgaaaactt tctccaccac gatagaacca ccgagcaaaa agccaaactg
     1261 taagcccatc atggtcacta ccgggatcat cgcgttacgt agcccgtgtt tgaggacaac
     1321 ccaggtttcg ctcacccctt tcgccctcgc ggtacgcata taatcttcgc ttaaaacatc
     1381 gacaaacgac gcgcgggtaa agcgcgccat cacggctgcc actgccgcgc cgagcgtcag
     1441 ggagggtaaa atgtagtgct gccaactgtc tgctcccacg gtaggcagcc agcccagttc
     1501 aacggagaat acctgaatta aaagcatccc cagcgcaaat gccggaaacg agatccccga
     1561 caccgcaatg gtcatactca atcgatccgg ccaacgatta cgccagacgg cggcgataat
     1621 tcccgccgcc ataccaaata tcaccgccca gaccatactg gttatggtca gccacagcgt
     1681 tggcataaag cggctggcaa tctcatcggc aaccggacga cgcgacacca tcgacaggcc
     1741 aaaatccccc tgcacagcat tgctgatata gtgccagaac tggtgataca gcggctgatc
     1801 caaccccagc tgctgacgca ccagttctat aacctgcgca tcagcttcgg gcccggcaat
     1861 caatcgcgcc ggatcgccgg gcagcatatg cacaaataaa aacaccagca ccgagacgat
     1921 aaacagcgtc ggaatcaacc ccagtaatcg tttgataacg taattaagca ttccactccc
     1981 tgcgttgctt attgcaaatc cgcgtcttca aagctgaagc cggtgtctgg catgatccaa
     2041 aaaccggtca ggtttttact gtgtgccgac accagttttt ctaccaccag cgggatccac
     2101 ggcgactctt gccagatgat atcctgcgcc gccttataca agcgggtctt ttccgccgga
     2161 tcattagttt tcagtgcctg agccaggaag tcgtccacct gtttattgct gtaaaatgcg
     2221 gtattaaaca gcgtcggtgg ccagttttgc gaggcgaaca gtggcgatag cgcccagtcc
     2281 gcttcgccgg ttgaagccga ccagccagtg tagaacatcc gcacgccgct ctctttttgc
     2341 cctttacctt caacttctgc cgcccgctgt ccggcatcca tcgcggtcac ctgggcttta
     2401 atcccgacct gcgctaactg ctgctgggta aattgcagca ctttctgcgc ggtgctgtgg
     2461 ttatgtgacg accacagcgt ggtactgaaa ccgttgggat atcccgcctc tttcagtaat
     2521 tcgcgcgctt tcactggatc gtaaggccac ggtttataac tttgcgcgta ggcgatactt
     2581 ggcggtacca caccagtagc tggcgttgca tagcccgcaa aggcaacttt caccagcgcc
     2641 ggacggttaa tggcgtaatt cagcgcctca cggaccttcg ggttatcgaa cggcttttgc
     2701 gtcacgttca tactgatata acgctgcata attgacggac tggccatcaa ctcgatattt
     2761 ttgtttttct ccagcagtgt ggcctgctcg tagggaatgg ggaaagcaaa ctgcgcttca
     2821 ccggtttgca gcattgccgc gcgggtgttg ttatccgcca ccggacgcca ggttatgctg
     2881 tccagtttgg gcaatcctgg ctgccagtaa cccgcgaatt ttttcacctt cacaaaatcg
     2941 gtctgattcc aggtatccag ttcatacggt ccggttccca ccggatgaaa accaatctcc
     3001 ttgccatatt tttccagcgc tgccggtgaa atcatcgcgg tcgccggatg ggcaagaata
     3061 ttaataaacg ctgagaacgg ctgtttgaga gtaatcttta ctgttgttgg atcgatcgcc
     3121 tccgttttgg caatattctt atacaggtta tagcgtttaa gatgattcgc cggatcgctg
     3181 gcccggtcca gattcgcttt taccgccgct gcgttgaaat cggtgccatc ctggaattta
     3241 attccttccc gcaatttcac ggtgtatgta aggccgtcat cggaaacggt ataactctcc
     3301 gccagcacgt ttttcagttt catctcttta tccagaccaa acagcccctg gtaaaacgat
     3361 ttcgctacgg cctgagataa cgtgtcattt gcgtcatacg gatcgagcgt ggtgaagttc
     3421 gatcctaccg ccaccaccac atctttggca gcgaatgcac aagatgccat caacgctgtc
     3481 gcaatgccca acgccactaa cccactacgg tgtacagctc ttgccatttt attctccgcc
     3541 tgaatgttat ctacgcatga atgcgtattc tgattgtggt tgtgcgacgt aatgccccgg
     3601 cccgacgcat tgcaacgaga cggctgccac ctcttcgcca cgcagatgaa tattgctggg
     3661 aagatcgtcc gacagcagta cacgctgcgg tcgttgtcgg gacggttcag cgaccggaac
     3721 tgccgccagt aatttacgcg tataaggatg ctgcgggttt tcgaagaccg cgcgccgtgg
     3781 accaatttca acaatttgtc cgagatacat caccgccaca cgatgactaa tccgctctac
     3841 cacggccata tcgtgggaga taaacagata cgcaatgccg aaatcacgct ggagatcgag
     3901 caacaagttg ataatctgcc cgcgaataga aacatccagc gccgagacgg cttcgtcggc
     3961 aatgatcact ttcggattca atgccaacgc gcgagcaatg cagatgcgct ggcgctgacc
     4021 gccggaaaac tcatgcgggt aacgccaggc atgttcaggt aacaggccca cgcgctccag
     4081 caaccacgca acgcgtgcag ccgcttcttt acctggcaat aaaccgtgta cacgcagcgg
     4141 ttcgataatc gaatcaccga tggtctgacg tgggtccagc gaagcgtaag ggtcctgaaa
     4201 aataaactga atatcccggc gcaatgcctg aagtttgccg ggtgacaagg tatcgattct
     4261 ctgaccgtta aagataattt cgccgccctg cgattcgacc aggcgcagca acgcccgccc
     4321 ggtagtggat ttaccgctgc cagactcgcc caccagcgat agcgtttcgc caggccagag
     4381 atcaaaactg actttctcaa cggcatgcac ttcccgcgtt acgcgattca acaaaccgct
     4441 gcgcaaaggg aaacgggtga caagattacg cactcgtaaa acaggttcgc catccaccac
     4501 cgttttctgc tcgatggggg gggcctgttt cgctggatgt tcaagcgata tcaacgggaa
     4561 acgtcgggga taatctaacc ctttcatcgc accaagttgc ggaacagcag ctaacagcgc
     4621 acgggtgtaa ggatgttgcg gtgcatgaaa aatctgttcg acggtacccg tttccaccgc
     4681 ctcgccctga tacatcacca gtacccgatc ggcaatctct gccaccacgc ccatatcgtg
     4741 agtgataaag ataacgccca tcgacatctc tttttgcaat actttgatta attgcaggat
     4801 ctgcgcctga atagtgacat ccagcgcggt ggttggctca tcggcaatca gcaccgccgg
     4861 gcggcatgac agcgccatcg caatcatcac tcgctggcgc atcccgccag agagttgatg
     4921 cggataacgt gaaagaatgg tttgtgcctc aggaatgcgt acctgatcca gcatccgctt
     4981 cgcctcgacc atcgcttctt cacgactggc gttctgatgc agacgaattg attcggcaat
     5041 ctgttcaccc acagtaaata ccgggttcag cgatgtcatc ggctcctgaa atatcatcgc
     5101 catatccgca ccgcgaacat ggcgcatttg tgcagcgctc tgctcgctaa gttcaatcac
     5161 ttcgcgactg cgccgctgca acagcatttt atcgcactgt actaaaccgc ccgcctgttc
     5221 caacaggcgc atcaatgcca acgcagtcac tgacttaccg gagccggatt cgccaacaat
     5281 tgccagcgtc tcaccgcgtt gcagactaaa agagagattg cggaccgcag ctattttctg
     5341 ctggtcctgc ataaaggcaa tattcagatt ttcaaccgcc agcacattac cggcatcaag
     5401 ttcatcactg tgtggcaacg gtgtcccctt tttcacggta gataccggtg gttggcgtat
     5461 cgcctgcgta gccccaggcg cgatacattc cttcggtgtt aaacggtagc gcgacattcc
     5521 cttcatggtc gatagcgatt aagccaccgc taccgccaag cgcagggagt ttttccatta
     5581 ctacccgctc gcaggcttcc gcgagactta atccgccgta atccattaac gcggcgatgt
     5641 catatgccgc cagcgcgcgg atgaagactt cgcccgtgcc ggtacaagaa accgccacac
     5701 tggcgttatt ggcgtagcat ccggcaccca ctaagggact atcgccaact cgtccgggta
     5761 atttattggt cattccgcct gtggacgtgg ctgccgccaa attgccgtct aaatccaacg
     5821 ccacggcccc cacggtgccc attttctgtt tttcatccag tggcgcaccg ctatggtcga
     5881 ggacggttgc cccttccttg cgcgctgcca gtagttgttc ataacgcaaa ggcgtggaga
     5941 aaatctccgg cgagacgcgc tccatgccac gagcaaacgc aaaattttct gccccttcgc
     6001 aatcatcatc acatgcgggc tttgctccat caccagccgg gcggcaagaa ccggattacg
     6061 cagatgacta acgcccgcca ccgcaccggc tttcagggtg ttaccatcca tcacacaggc
     6121 gtccagttca tgggtttcat cacgcgtaaa gacagcgcca attccggcgt taaacagtgg
     6181 acactcttcc agcagacgca ccgcttccgt caccacatcc agcgcacttt cgcccgcttc
     6241 cagcattttc tgcccggttt caacaatggc agacaacgcc tcaatgtagc gtaattcctg
     6301 ttgcagactc atctgcgcgc ggctaattgc acctgcgcca ccatgaattg caatgactgc
     6361 tttgcccata gtattcgtcc attaagggtg gctcgccact tttgctataa atatcagaat
     6421 ttttatgaat tactcattca atttttgaat atagaaagat ggatatggca tgtaaaggca
     6481 ggagccaacg ctacgcccga ggtggcatac agtctgtggc gtttttctga cataataggc
     6541 aaattcgatt ttgcctccga aggagtgttt tcatggaatt taccaccgga ttgatgtcgc
     6601 tcgacaccgc gcttaatgag atgctttctc gcgtcacccc actgaccgcc caggaaacgc
     6661 tgccactggt acagtgtttt ggtcgtattc tggcgagcga tgtcgtttcg ccgcttgatg
     6721 ttccggggtt tgataactcc gcaatggacg gctacgcggt gcgtttagcc gatattgcct
     6781 ccaggcaacc gctgcccgtt gccggtaaat cctttgccgg tcagccgtac catggtgaat
     6841 ggcctgcggg tacctgcatt cgtattatga ccggtgcgcc ggtgccggaa ggctgcgaag
     6901 cggtggtgat gcaggagcag actgaacaaa cggacaatgg cgtgcgtttt actgctgaag
     6961 tgcgtagcgg gcaaaatatt cgccgtcgcg gtgaagatat ctctgcaggt gcggttgttt
     7021 tcccggcggg aactcgcctg actaccgctg aactgccagt gattgcttca ctggggattg
     7081 ccgaagttcc ggtgattcgt aaagtgcgtg tagcgctttt ttctaccggt gatgaactcc
     7141 agttgcccgg tcagccactg ggcaatggcc aaatctacga taccaaccgt ctcgccgtac
     7201 acctgatgtt agaacagttg ggatgcgagg taatcaactt agggattatc cgcgacgatc
     7261 cacatgccct gcgcgccgca tttattgaag ccgacagcca ggcggatgtg gtgatcagtt
     7321 ccggcggtgt ttcagtgggt gaagcggatt acaccaaaac cattcttgaa gagctggggg
     7381 agatcgcctt ctggaagctg gcgattaaac cgggtaaacc gttcgcgttc ggtaaacttt
     7441 ctaatagctg gttctgcggc ctgccgggca acccggtttc agcgacgctg accttctatc
     7501 aactggtaca gcctttgctg gcaacactaa gcggcaacac cgccagcggc ctgcccgcgc
     7561 gccagcgcgt acgcacagcg tcccgcctga aaaaaacgcc aggacgtctt gatttccagc
     7621 gcggcgtgct gcaacgcaac gccgacggtg aacttgaagt gacgaccacc ggacatcagg
     7681 gttcacatat atttagctcc tttagtctcg gcaactgctt tatcgtgctg gaacgcgatc
     7741 gcggcaatgt ggacgtgggc gaatgggtgg aagtagaacc gtttaacgcg ttgttcggag
     7801 gcctgtaatg gcggaactca gcgatcagga gatgctgcgc tacaaccggc aaatcattct
     7861 gcgcggtttt gattttgacg gtcaggaggc gctaaaagat tctcgtgtgc tggtagtcgg
     7921 cctcggtgga ctcggctgcg cagcttcgca gtatctggca agcgccggtg tcggcaacct
     7981 gacgctgctc gacttcgaca cggtttcgct ctcgaatctg caacgccaga cactgcacag
     8041 tgatgccacg gtcgggcaac cgaaggtgga atccgcccgt tacgccctga cgcggatcaa
     8101 cccacatatc gcgattacgc cagtcaatgc actgctagat gacgcagaac ttgcagcatt
     8161 gattgctgaa cacgatctgg tgctcgactg tacggataac gttgcggtac gtaatcaact
     8221 gaacgcaggc tgttttgccg cgaaggtgcc gctggtttcc ggcgcggcaa ttcgtatgga
     8281 agggcaaatc acggtcttca cttatcagga cggcgaaccg tgctatcgct gccttagccg
     8341 tttgtttggc gagaacgcat taacctgcgt agaagcaggc gtgatggcac cgttgatcgg
     8401 cgtaattggt tcgttgcagg caatggaagc gatcaaactg ctggcaggtt atggcaaacc
     8461 ggccagcggg aaaatcgtca tgtacgatgc gatgacctgt cagtttcgcg aaatgaaact
     8521 gatgcgcaat ccggggtgtg aggtgtgcgg gcagtaattg ccacctccag gcctgataag
     8581 acgcgacaag cgtcgcatca ggcatttgct ccagacgcca gatgcggcgt gaacgcctta
     8641 tctggcctac ggttcgggca cagatttcca agcgcggcag gcatcggtgc actccgccac
     8701 gtcatcagca atttatgagg atgcagaacg tccggttaaa tcgacgttct gccaaacgct
     8761 cccagccagt cctgttcaaa cttcgccaca gcggcttcca ccgccggata gctaatcatc
     8821 tgttgtgcca catccagtgg cagcgtaatt gattcacatc ctgccagtaa gcagtccagc
     8881 gcctgacgcg gggttttgaa actcgctgcc agcactttcg cctgcggcgc atgcattttc
     8941 aataactggt gtaagtcggt cacagtctga atgccgctac cgccctgagc atcaatacga
     9001 ttaacgtaag gcgcaacata ttccgcacct gccagcgccg acagcagccc ttgtgctgcg
     9061 ccatataccg cggttcccag cgttggaatc ccttccgctt ttaacatctt aatagctgcc
     9121 agcccctcgg cggtcaccgg aactttcacc acgatatccg caataataga acgcaattta
     9181 cgtgcgtcat tcaccatccc ttcagcagtg gtagccatta cctgggcaaa cagacgcccc
     9241 tgaccgccca tcgcttcatg aagttgcgga agcacaacat ccagcggttt tttacccgcg
     9301 gcgataatgc ttgggttagt ggtcacaccc gccagcggaa aaatacgtga cagcgccttc
     9361 accgcaacaa cgtatgaagt atccagatac agttccataa ccatcctcaa aatgtacttt
     9421 ttgtctgaat gtagactatc acgggaaaac tctctgaaca gttgacgcac atcaatctaa
     9481 ctttcattcg aaagtatttt aatgtttgaa cgaaacagag aggcaactat gattttcaat
     9541 attcagcgct actcgaccca tgatggcccc ggtatccgca cggtcgtatt tcttaaaggc
     9601 tgttcgctgg gctgccgctg gtgtcagaac ccggaaagcc gcgcccgcac gcaggatctg
     9661 ctgtatgacg cacgactgtg tctggaaggc tgcgagctgt gcgctaaggc tgcgccggaa
     9721 gtgattgagc gcgcgctgaa tggtttgctt attcatcggg aaaagttaac cccggagcat
     9781 ctgacggcgt taaccgactg ctgtccgaca caggcattaa ccgtgtgtgg tgaagtgaaa
     9841 agcgttgagg agatcatgac gaccgttctg cgcgataaac cgttttacga tcgcagcggc
     9901 ggcggtttaa cgctttcggg tggtgagccc tttatgcagc cggaaatggc gatggcgcta
     9961 ctgcaagcca gccacgaggc aggcattcat actgcggtag aaacctgtct gcatgtgccg
    10021 tggaaatata tcgccccttc tctgccctat atcgatctgt ttcttgccga tttaaaacac
    10081 gttgccgacg cgccgtttaa acagtggacc gacggtaacg ccgccagagt gctggataac
    10141 ctgaaaaaac tcgccgcagc gggcaaaaaa atcattatcc gcgtgccgct gattcagggc
    10201 tttaatgccg acgaaacctc tgtaaaagcc attaccgatt ttgccgccga cgagctgcac
    10261 gttggcgaaa ttcattttct gccctaccac acgctgggca tcaacaaata tcacttactt
    10321 aatctgccct atgacgcccc ggaaaaaccg cttgatgcgc cagaactgct cgactttgcc
    10381 cagcagtatg cctgccagaa agggttaacc gcgaccttac gaggataaca accatgacca
    10441 cactgaaact ggacacgctc agcgaccgca ttaaagcgca caaaaatgcg ctggtgcata
    10501 ttgtgaaacc gccagtctgt accgagcgcg cgcagcacta taccgagatg tatcaacaac
    10561 atctcgataa gccgatcccg gtacgtcgcg cgctggcact ggcgcatcac ctggcgaatc
    10621 gcaccatctg gatcaaacac gatgagttga tcattggcaa ccaggcaagc gaagttcgcg
    10681 ccgcgccgat cttcccggaa tatactgtct cgtggatcga aaaagagatt gatgatctgg
    10741 cagatcgtcc cggtgctggc tttgcggtga gcgaagagaa caaacgcgtt ctgcatgaag
    10801 tgtgcccgtg gtggcgcggt cagaccgtac aggatcgctg ctacggcatg tttaccgatg
    10861 agcaaaaagg tctgctggcg accggaatca ttaaagcgga aggcaatatg acctccggcg
    10921 atgcgcacct ggcggtgaat ttcccgctgc tgctggaaaa aggtcttgat ggtctgcgtg
    10981 agaaagtagc ggaacgtcgc tcgcgcatca atctgacggt gctggaagat ttacatggcg
    11041 aacaattcct gaaagcgatt gatatcgtgc tggtggcagt cagtgaacac attgaacgtt
    11101 tcgctgccct ggcgcgtgaa atggctgcta ccgaaactcg cgaaagccgt cgcgatgaac
    11161 tgctgacgat ggcagaaaac tgcgatctta tcgcccacca gccgccgcag actttctggc
    11221 aggcgctgca actgtgttac ttcatccaat tgattttgca gatcgaatct aacggtcact
    11281 cggtatcgtt tggtcgtatg gaccagtatc tctacccgta ctatcgccgc gacgttgaac
    11341 tcaaccagac gctggatcgc gaacacgcca tcgagatgct gcatagctgc tggctgaaac
    11401 tgctggaagt gaacaagatc cgctccggct cacactcaaa agcctctgcg ggaagtccgc
    11461 tgtatcagaa cgttaccatt ggcggacaaa atctggttga tggtcaacca atggatgcag
    11521 tgaatcccct ctcttacgcg atcctcgaat cctgcggtcg cctgcgttcc actcagccta
    11581 acctcagcgt gcgttaccat gcaggaatga gtaatgattt cctcgacgcc tgcgtacagg
    11641 tgatccgttg cggcttcggg atgccggcgt tcaacaacga cgaaatcgtg atcccggaat
    11701 ttattaaact cggtattgaa ccgcaggacg cttacgacta cgcagcgatt ggttgtatcg
    11761 aaaccgccgt cggtggcaaa tggggctatc gctgtaccgg catgagcttt atcaacttcg
    11821 cccgcgtgat gctggcggcg ctggaaggcg ggcgtgatgc caccagcggc aaagtgttcc
    11881 tgccacaaga aaaagcgttg tcggcaggca actttaacaa tttcgatgaa gtaatggacg
    11941 cgtgggatac gcaaatccgt tattacacgc gtaaatcaat cgaaatcgaa tatgtcgtcg
    12001 acaccatgct ggaagagaac gtacacgata ttctctgctc ggcgctggtg gatgactgta
    12061 ttgagcgagc gaaaagtatc aagcaaggcg gcgcgaaata tgactgggtt tctggcctgc
    12121 aggttggcat tgccaacctc ggcaacagcc tggcggcagt gaagaaactg gtatttgaac
    12181 agggcgcgat tggtcagcaa cagctggctg ccgcactggc ggatgacttc gatggcctga
    12241 ctcatgagca actgcgtcag cggctgatta acggtgcgcc gaagtacggc aacgacgatg
    12301 ataccgtcga tacgctgctg gctcgcgctt atcagaccta tatcgacgaa ctgaaacagt
    12361 accataaccc gcgctacggt cgtggtccgg ttggcggcaa ctattacgcg ggtacctcgt
    12421 ctatctccgc taacgtaccg tttggcgcgc aaactatggc aacaccggac gggcgtaaag
    12481 cccacacccc gctggcagaa ggcgcaagcc cggcttccgg tactgatcat cttggcccta
    12541 ctgcggtcat tggctcggtg ggtaaactgc ctacggcagc gattctcggc ggcgtgttgc
    12601 tcaaccagaa actgaatcca gcaacactgg agaacgaatc tgacaagcag aaactgatga
    12661 tcctgctgcg caccttcttt gaagtgcata aaggctggca tattcagtac aacatcgttt
    12721 cccgcgaaac gctgctggag gcgaaaaaac atccggatca gtaccgcgat ctggtagtgc
    12781 gtgtcgcggg ctattccgcc ttcttcaccg cgctctctcc agacgcacag gacgatatca
    12841 tcgcccgtac tgaacatatg ctgtaatctc ctcggcccgg cggcgtcgtc gccgggctaa
    12901 atcattcaca tcatcaattt catccttact ttcattcgaa atataatttg tgctctgcgt
    12961 cacattgtta ttacactgtt acggtttcat tttcgagcca ggagcacacc tatgagcgta
    13021 aaagttatcg tcacagacat ggacggtact tttcttaacg acgccaaaac gtacaaccaa
    13081 ccacatttta tggcgcaata tcaggaactg aaaaagcgcg gcattaagtt cgttgtcgcc
    13141 agcggtaatc agtattacca gcttatttcg ttctttcctg agctaaagga tgagatctct
    13201 tttgtcgcgg aaaacggcgc actggtttac gaacatggca agcaactgtt ccacggcgaa
    13261 ctgacccgac atgaatcgcg gattgttatt ggcgagttgc taaaagataa gcaactcaat
    13321 tttgtcgcct gcggtctgca aagtgcatat gtcagcgaaa atgcccccga agcatttgtc
    13381 gcactgatgg caaaacacta ccatcgcctg aaagctgtaa aagattatca ggagattgac
    13441 gacgtactgt tcaagttttc gctcaacctg ccagatgaac aaatcccgtt agtgatcgac
    13501 aaactgcaca tagcgctcga tggcattatg aaacccgtca ccagtggttt tggctttatc
    13561 gacctgatta ttcccggtct acataaagca aacggtattt cgcggttact gaaacgctgg
    13621 gatctgtcac cgcaaaatgt ggtagcgatt ggcgacagcg gtaacgatgc ggagatgctg
    13681 aaaatggcgc gttattcctt tgcgatgggc aatgctgcgg aaaacattaa acaaatcgcc
    13741 cgttacgcta ccgatgataa taatcatgaa ggcgctctga atgtgattca ggcggtgctg
    13801 gataacacat ccccttttaa cagctgacct ctctaccgga gccgtgcgct ccggttttcc
    13861 cttcttttta catcctgtac attacatcaa atcattaaac ttgcattaac ttatttataa
    13921 cttatattgt cacatgcaaa tcagaatggt ttcgaatgaa agttaatgag aggtggttat
    13981 ggcttctact tttaccagcg acacattgcc tgccgatcac aaagcagcta tccgtcagat
    14041 gaagcacgcg ctgcgggcgc agcttggcga cgtccagcag atctttaatc agctaagcga
    14101 tgacattgcc acgcgagtgg ctgaaatcaa cgcactcaaa gcacagggcg atgccgtctg
    14161 gccggtgctg tcttatgccg atatcaaagc aggtcatgtt actgcagagc agcgcgaaca
    14221 gattaaacgt cgcggttgtg cggtgataaa aggccatttc ccccgcgaac aagcgctagg
    14281 ctgggatcag tcgatgctgg actatctgga ccgcaaccgc tttgacgagg tctacaaagg
    14341 ccccggcgat aatttcttcg ggacgctcag cgcttcacgt cccgagattt accccatcta
    14401 ctggtcgcag gcgcaaatgc aggcccgcca gagtgaagaa atggcgaatg cgcagtcgtt
    14461 tctcaatcgt ctgtggacat ttgaaagtga tggaaagcaa tggtttaacc cggatgtgag
    14521 cgtcatctac cctgaccgta tccgccgccg tccgcccgga acgacctcca aaggtcttgg
    14581 agcgcatacc gactccgggg cgctggaacg ctggctgctt ccagcgtatc agcgcgtttt
    14641 cgccaacgtc tttaatggca atctggcgca atatgatccc tggcatgcgg cacatcgtac
    14701 ggaagttgaa gagtacacgg tggacaacac caccaaatgt tccgtgtttc ggacattcca
    14761 gggctggaca gcgctctctg atatgctgcc tggtcagggg ttgctgcacg ttgtgcccat
    14821 tcctgaagcc atggcgtacg tactgttacg tccgctgctt gatgatgtgc cggaggatga
    14881 actgtgcggc gtagcgcccg gaagagtgtt gccggtatca gagcaatggc atccactgtt
    14941 aattgaggcg ttaaccagca ttccaaaact cgaggccgga gactccgtct ggtggcactg
    15001 cgacgtcatc cattccgttg cccccgttga aaatcaacag ggttggggca acgtgatgta
    15061 cattcctgcg gcaccgatgt gcgagaaaaa tcttgcctac gcgcacaagg tgaaggccgc
    15121 actggaaaaa ggcgcatcgc cgggcgactt cccgcgcgag gactatgaaa caaactggga
    15181 aggacgcttt acgcttgccg acctcaacat tcacggtaag cgagcgttgg gcatggatgt
    15241 ttgagagtta atggaagtga attgatgtgg gattaagaaa atcgattcga cgttactcag
    15301 attgctgaca agtgcgcttt cttaatgcct gatgcgctac gcttatctgg cctacaaagt
    15361 cctgcagatt caatatatta caggtact
//
LOCUS       DAZGVQ010000120        14711 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000120 DAZGVQ010000000
VERSION     DAZGVQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14711
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.121"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..241
                     /locus_tag="ACHLAG_003688"
     CDS             <1..241
                     /locus_tag="ACHLAG_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFP0656839.1"
                     /translation="YDWMLESGDNICSIPSVSGEDRILQAMIAAFFLLTPLIILILRK
                     LFMREMFEFWVYVFSLGICLVCGWWLFWGRFIFCY"
     gene            complement(287..1636)
                     /gene="qseC"
                     /locus_tag="ACHLAG_003689"
     CDS             complement(287..1636)
                     /gene="qseC"
                     /locus_tag="ACHLAG_003689"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFP0656840.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHDHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPREDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(1633..2292)
                     /gene="qseB"
                     /locus_tag="ACHLAG_003690"
     CDS             complement(1633..2292)
                     /gene="qseB"
                     /locus_tag="ACHLAG_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFP0656841.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            2444..2836
                     /gene="ygiW"
                     /locus_tag="ACHLAG_003691"
     CDS             2444..2836
                     /gene="ygiW"
                     /locus_tag="ACHLAG_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFP0656842.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            2889..3371
                     /locus_tag="ACHLAG_003692"
     CDS             2889..3371
                     /locus_tag="ACHLAG_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP0656843.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            3917..6175
                     /gene="parC"
                     /locus_tag="ACHLAG_003693"
     CDS             3917..6175
                     /gene="parC"
                     /locus_tag="ACHLAG_003693"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFP0656844.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            6408..7145
                     /gene="plsC"
                     /locus_tag="ACHLAG_003694"
                     /gene_synonym="parF"
     CDS             6408..7145
                     /gene="plsC"
                     /locus_tag="ACHLAG_003694"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFP0656845.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            7220..8632
                     /gene="ftsP"
                     /locus_tag="ACHLAG_003695"
     CDS             7220..8632
                     /gene="ftsP"
                     /locus_tag="ACHLAG_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFP0656846.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            8743..10962
                     /locus_tag="ACHLAG_003696"
     CDS             8743..10962
                     /locus_tag="ACHLAG_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFP0656847.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(11005..11262)
                     /gene="yqhH"
                     /locus_tag="ACHLAG_003697"
     CDS             complement(11005..11262)
                     /gene="yqhH"
                     /locus_tag="ACHLAG_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFP0656848.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(11313..12239)
                     /locus_tag="ACHLAG_003698"
     CDS             complement(11313..12239)
                     /locus_tag="ACHLAG_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFP0656849.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            complement(12439..13266)
                     /gene="dkgA"
                     /locus_tag="ACHLAG_003699"
                     /pseudo
     CDS             complement(12439..13266)
                     /gene="dkgA"
                     /locus_tag="ACHLAG_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
     gene            complement(13371..14534)
                     /gene="yqhD"
                     /locus_tag="ACHLAG_003700"
     CDS             complement(13371..14534)
                     /gene="yqhD"
                     /locus_tag="ACHLAG_003700"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFP0656850.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
BASE COUNT         3389 a         3770 c         3912 g         3640 t
ORIGIN      
        1 gtatgactgg atgcttgagt cgggagacaa tatttgcagt ataccttccg tttcaggcga
       61 agatcgtata ttgcaagcaa tgattgcagc attttttctt cttacgccac ttattattct
      121 tatcttaaga aaactcttta tgcgggaaat gttcgagttt tgggtatatg tattttcgct
      181 cggtatttgt ctggtttgtg gttggtggct tttttgggga cggtttatat tttgctatta
      241 gtaaaatcta tgagagtcgt tttaacggct ctcatagaca gagaagttac cagcttacct
      301 tcgcctcaaa tccaccttgt tccgcattcc caaactcaac attcatgcca tgcaatttgg
      361 cgattcgctg gacaatcgat agcccaagtc cactgccggt agcggtttgt ccgggggggc
      421 gatagaagcg ttcgccaatt cgcgccagag cctctggcgt cacaccgggg ccgttatccc
      481 tcacgatgaa attatcagca ttcagcgtga cgtctatcac gctgccctgt ggactgtagc
      541 gcacggcgtt atccagcaaa tttcgcacca acaaacttag caatagcggt tgtccggtgc
      601 gtttgatgcc gtgggcattg agtgtcagtc gcacgtcaat tttcgcctgc tgcgccgtgt
      661 ggtaaatatc catcaccgac gattgcagga gatcttcacg cgggatctcc gcgacgtcct
      721 gaaggttatc cagtgagtcc agccgcgata gagtgagcag ttgatcaacc agacgagtag
      781 cgcgatcgat cccgtaatgt aattgaagca gcgctttttt ccgcgcttgc ggatcatcgt
      841 cagaaagctg cgcaacttcg gtttgcactt tcagcgccgt taacgggcta cgtagttcgt
      901 gagctgcgtc ggaggtaaag cgtcgttcac gaaccatcat cgcatgtgtg cgggcgaaca
      961 gttgatttag cgactcaacc aacggacgca cttcgctggg tacgccagtt gcgtttagtg
     1021 gtttttccga gtcagggtca cgcatacgca gcgccagcgc cagtttgttc agcggcgcga
     1081 gttcacgacc caatagtacc atcatgatga ttaacataat cggcagtgcg accagccacg
     1141 ggatcaattg cccggcaaca atcgccagcg ccatgtcttc acggtattcc cattcctgac
     1201 caacaacgat gcgatatttg ccatcaggtg aggtcatcca gacaaaacgc cattgatctt
     1261 tatcgcccac cagttgcccg tcagcaaaac cttcccgttg atagctataa ggaatatctt
     1321 ctccgttatc gccatcgtta aggaccattc tgccgtcgtg ggtaaagatg gcaaaggtca
     1381 gcgcatcgtc atcaacatga tcgtgtttta atttatttgg cgtctgtgcc atgcgatccg
     1441 ccgcgttgat ttcgttgaga tcgagcgtac ttaaccgctt ggcaaacagc atcagttggg
     1501 tgtcgaacaa ttcatcgacg ttatccgttg tttgtttcca ggcgacaaag ctggaaagca
     1561 gccaggtcac cgaggccaga attaaaaaga ttagcgtcag cctgacgcgc agactaagac
     1621 gttgggtaaa tttcatttct cacctaatgt gtagccaata ccatgcacgg tacgaataaa
     1681 atcactaccg agtttgcgtc gcagatgatg cacatgcact tcaacggcat tactggtgac
     1741 ctcttcgtcc caggtataca gtttctcttc aatcagtttg cgcggcagta cccgaccagc
     1801 gttacgcatc agtaattcca gcagggcaaa ttcttttggt ttcagtgtta agggttcgcc
     1861 agccagcgtg gcgatacgtt tgccggggtc gagcatgacg ttaccgtggc gcagctcgtt
     1921 gctggcctgg ccgttggttc ggcgcatcag agcttccagc ctggcggcga cttctatcaa
     1981 cgcaaaaggt ttacacagat aatcgtcagc tcccagacgc agcccttcta cacgttcctc
     2041 cagcgcatcg cgcgcggtca ggatcagtac cggctcacgc tgaccttttt ctcgccattc
     2101 gcgcaaaata tcgcgaccat ccatgcctgg taaggttaaa tccaggatca ccgcatcata
     2161 aggcgcgcta taaagcgcct cttttccctg acgaccttgt gtaaaccagt cgacgctaaa
     2221 acccatttta ctaaggcccg ttttgatgcc gtcgccaatc agcatgtcat cttctatcag
     2281 taaaattcgc attttttcat ccctgcgata accgtatttg ctgccagtaa accgtgttgt
     2341 aacgcgctct gtacagcact aaattttatt tttttccctt aagaagttgt taaggactat
     2401 cttgttaaat gctcggaaac agacattaaa gggagtaata aacatgaaaa aattcgcagc
     2461 agtaatcgca gtaatggccc tgtgcagcgc accggtgatg gcagcagagc agggcggttt
     2521 ttctggccca tcggcaacgc aaagtcaggc cggaggattc caggggccga acggcagcgt
     2581 aacgaccgta gaaagcgcaa aatccctgcg tgacgacacc tgggtaaccc tgcgcggcaa
     2641 tatcgttgaa cgcatctctg acgatctcta cgtgtttaaa gatgccagcg gtactatcaa
     2701 tgttgatatc gaccacaaac gctggaacgg tgtgacggtg acgccgaaag atacggttga
     2761 gattcagggt gaagtcgata aagactggaa ttctgttgaa attgacgtca aacagatccg
     2821 caaagtaaat ccgtaattgt taccgctccc gggacgcgtt cccgggaata atttcgcagg
     2881 gaggcaaaat gacaaacctg acactggatg taaacattat cgatttccca tcaatccctg
     2941 tggcgatgtt gccgcaccgc tgtagccctg agttgctcaa ctacagtgtg gcgaaattta
     3001 tcatgtggcg taaagagacg gggctttctc ctgttaacca aagccagact tttggcgtcg
     3061 cctgggacga ccctgccacc accgcaccgg aagcgtttcg ctttgatatc tgcggcagcg
     3121 ttagcgaacc gattcccgat aatcgttatg gtgtgagcaa tggtgaactt accggtggac
     3181 gttatgccgt ggcccgccac gttggcgagc tggacgatat ttcacacacg atatggggca
     3241 ttattcgcca ctggctgcct gcaagcggcg agaaaatgcg taaagcaccg attctgtttc
     3301 actacaccaa tcttgccgaa ggggtgacag agcagcgact ggaaacggat gtttatgtgc
     3361 cgttggcgtg atggggaatc tttaccggag tgtgagccaa atcactcgcg ataagtttga
     3421 gcgaacaatg ccacaggcgt attgttgttg ggcttataat ttctccggta aggaatacca
     3481 tgtatacgcg aaatttatta tccggtgcgc cgtaagccgt ctcgggggta tccatgagca
     3541 agccgcactt atggcaggag gcgcggggca ctggtggaga cacagctaaa ctgggctgaa
     3601 ccgtatactt acagtcgact actttgatgc tttaggcttc cctggactcg cataacctca
     3661 acttctcgaa tcgcccggag cggaccccca tgccgagtgg tgtggcatgg gcgcaacctt
     3721 gtgctgccct ctatgccgat caagtctaca gcaaagagtt gtatatcaag gcacattgat
     3781 gcctttatcg ccgtcggagc acgtccggcg gcgctattca cgctgatctc ctgtgactcg
     3841 acgcggcaga taatgtagta tctccggcaa tattgcccct ttgaaggctg gcgaataagt
     3901 tgaggaatca gaattaatga gcgatatggc agagcgcctt gcgctacatg aatttacgga
     3961 aaacgcctac ttaaactact ccatgtacgt gatcatggac cgtgcgttgc cgtttattgg
     4021 cgatggtctt aaacctgttc agcgccgcat tgtgtatgcg atgtctgaac tgggcctgaa
     4081 tgccagcgcc aaatttaaaa aatcggcccg taccgtcggt gacgtactgg gtaaatacca
     4141 tccgcacggc gatagcgcct gttatgaagc gatggtcctg atggcgcagc cgttctctta
     4201 ccgttatccg ctggttgatg gtcaggggaa ctggggcgcg ccggacgatc cgaaatcgtt
     4261 cgcggcaatg cgttacaccg aatcccggtt gtcgaaatat tccgagctgc tattgagcga
     4321 gctggggcag gggacggctg actgggtgcc aaacttcgac ggcactttgc aggagccgaa
     4381 aatgctgcct gctcgtctgc caaacatttt gcttaacggc accaccggta ttgccgtcgg
     4441 catggcgacc gatattccac cgcataacct gcgtgaagta gctcaggcgg caattgcatt
     4501 aatcgaccag ccgaaaacca cgctcgatca gctgctggat atcgtgcagg ggccggatta
     4561 tccgactgaa gcggaaatta tcacttcgcg cgccgagatc cgtaaaatct acgagaacgg
     4621 acgtggttca gtgcgtatgc gcgcggtgtg gaagaaagaa gatggcgcgg tggttatcag
     4681 cgcattaccg catcaggttt caggtgcgcg cgtactggag caaattgctg cgcaaatgcg
     4741 caacaaaaag ctgccgatgg ttgacgatct gcgcgatgaa tctgaccacg agaacccgac
     4801 ccgtctggtg attgtgccgc gttccaaccg cgtggatatg gatcaggtga tgaaccacct
     4861 cttcgctacc accgatctgg aaaagagcta tcgcattaac ctcaatatga tcggtctgga
     4921 tggtcgtccg gcggtgaaaa acctgctgga aatcctctcc gaatggctgg tgttccgtcg
     4981 cgataccgtg cgccgccgac tgaactatcg tctggagaaa gtcctcaagc gcctgcatat
     5041 cctggaaggt ttgctggtgg cgtttctcaa catcgacgaa gtgattgaga tcattcgtaa
     5101 cgaagatgaa ccgaaaccgg cgctgatgtc gcggtttggc cttacggaaa cccaggcgga
     5161 agcgatcctc gaactgaaac tgcgtcatct tgccaaactg gaagagatga agattcgcgg
     5221 tgagcagagt gagctggaaa aagagcgcga ccagttgcag ggcattttgg cttccgagcg
     5281 taaaatgaat aacctgctga agaaagaact gcaggcagac gcgcaagcct acggtgacga
     5341 tcgtcgttcg ccgttgcagg aacgcgaaga agcgaaagcg atgagcgagc acgacatgct
     5401 gccgtctgaa cctgtcacca ttgtgctgtc gcagatgggc tgggtacgca gcgctaaagg
     5461 ccatgatatc gacgcgccgg gcctgaatta taaagcgggt gatagcttca aagcggcggt
     5521 gaaaggtaag agcaaccaac cggtagtgtt tgttgattcc accggtcgta gctatgccat
     5581 cgacccgatt acgctgccgt cggcgcgtgg tcagggcgaa ccactcaccg gcaaattaac
     5641 gttgccgcct ggggcgaccg ttgaccatat gctgatggaa agcgacgatc agaaactgct
     5701 gatggcttcc gatgcgggtt acggtttcgt ctgcaccttt aacgatctgg tggcgcgtaa
     5761 ccgtgcaggt aaggctttga tcaccttacc ggaaaatgcc catgttatgc cgccggtggt
     5821 gattgaagat gcttccgata tgctgctggc aatcactcag gcaggccgta tgttgatgtt
     5881 cccggtaagc gatctgccgc agctgtcgaa gggcaaaggc aacaagatta tcaacattcc
     5941 atcggcagaa gccgcgcgtg gagaagatgg tctggcgcaa ttgtacgttc tgccgccgca
     6001 aagcacgctg accattcatg ttgggaaacg caaaattaaa ctgcgcccgg aagagttaca
     6061 gaaagtcact ggcgaacgtg gacgccgcgg tacgttgatg cgcggtttgc agcgtatcga
     6121 tcgtgttgag atcgactctc ctcgccgtgc cagcagcggt gatagcgaag agtaatatgc
     6181 cggggagggc ctgtttcctc ccgcttgcaa ggaacgccgg atgaaacgtt aagtcgtcat
     6241 ccggcagcgt attcaacccc cggaaacgat aaaaattccc ttgaaaccgt tgtttattca
     6301 tgcgttgcga ttaacaatac gcttttccag agagcggctt ttaacaatgc cctaacctga
     6361 tttcaggtga cgtacaatgc cagtctctca gaccttcaga gggtgttatg ctatatatct
     6421 ttcgtcttat tattaccgtg atttacagca tcttagtctg tgtattcggc tccatttact
     6481 gccttttcag cccgcgtaac ccgaaacatg tggccacctt tgggcatatg tttggccgtc
     6541 ttgcgccgct gttcggcctg aaagttgagt gccgcaaacc tgcagatgct gaaagctacg
     6601 gcaatgctat ctatatcgct aaccaccaga acaactatga catggtgaca gcatcgaaca
     6661 ttgtgcaacc gccgacggtg acggtaggta aaaagagctt gctgtggatc cccttcttcg
     6721 ggcagttgta ctggttaacc ggcaacttat tgatcgacag aaacaatcgc actaaagctc
     6781 acggcaccat tgcggaagtc gtgaatcatt tcaaaaaacg ccgtatttcc atctggatgt
     6841 tcccggaagg aacccgcagc cgtggtcgcg gcctgctacc gttcaagact ggagcatttc
     6901 acgcggcaat tgcggcgggc gtcccgatta ttcccgtgtg cgtctctaca acttcgaata
     6961 agattaatct taatcgactg cacaacggtc tggtgattgt cgaaatgctg ccgccaattg
     7021 acgtcagtca gtatggcaaa gatcaggttc gtgagctggc tgcccattgt cgttcgataa
     7081 tggaacaaaa aatcgccgag ctcgataaag aagtcgcaga acgcgaagcc gccggaaaag
     7141 tttaagtcgg caatctgtat ttttgcgggg aacactttcc tgcacggtat tactttagcc
     7201 agttttacat ggagcaaata tgtcactcag tcggcgtcag ttcattcagg catcggggat
     7261 tgcactttgt gcaggcgctg ttcccctgaa ggccagcgca gccgggcaac agcaaccgct
     7321 acccgttccg ccgctacttg aatctcgccg tgggcaaccg ctgtttatga ctgtacaacg
     7381 tgcgcactgg tcatttacgc cagggacacg cgcgtcggtc tggggaatca atggtcgtta
     7441 cctggggccg actatccgcg tctggaaggg cgacgatgtt aagcttattt acagcaaccg
     7501 cctgacagaa aatgtctcaa tgacggtggc cgggctacag gtaccaggcc cgctgatggg
     7561 cggtccggca cggatgatgt cgccaaacgc tgactgggca cccgtactgc ccattcgcca
     7621 gaacgcagct actctgtggt atcacgccaa tactcccaac cgcacggctc agcaggtcta
     7681 taacggcctt gccggaatgt ggctggtgga agatgaagtc agcaagtcgc tgcctatccc
     7741 caaccattat ggtgtggatg attttccggt cattatccag gataaacggc tggataactt
     7801 tggtacgcca gaatacaacg aaccgggaag cggcggcttt gttggtgata cgctgctggt
     7861 taacggtgta caaagcccgt acgttgaagt ctcgcgtggc tgggtgcgct tgcgactgct
     7921 gaacgcgtcg aactctcgtc gctatcaact acagatgagc gatggtcgcc cgttacatgt
     7981 gatttctggc gatcagggat tcctgcctgc tcctgtatcg gtgaagcaac tttcgctggc
     8041 accgggcgag cgccgcgaga ttctggtgga tatgagcaac ggcgatgaag tgtcgatcac
     8101 ctgtggcgaa gcggcgagca ttgttgatcg tattcgcggc ttctttgagc catccagtat
     8161 tctggtttct accctggtgc taacgctgcg cccaaccggc cttctgccgc tggtcacaga
     8221 cagtcttccg atgcgcttgc tgccaactga aatcatggct ggttcgccaa ttcgcagtcg
     8281 cgatatcagt ctgggtgatg acccgggtat taatggacag ctgtgggacg tcaaccgtat
     8341 tgatgtcacc gcgcagcaag gaacgtggga acgctggacg gtacgcgcgg acgagccgca
     8401 agcgttccat attgaaggcg taatgttcca gatccgtaac gtgaatggcg cgatgccgtt
     8461 cccggaagac agaggctgga aagataccgt ttgggttgac ggacaagtgg agctgcttgt
     8521 ttatttcggt cagccttcct gggcgcactt cccgttctac ttcaacagtc agacgctgga
     8581 aatggcggac cgtggctcga tagggcaact tttggtcaat ccggtaccgt aatcttcata
     8641 ctcgcccgag gagggcgagt atggaatacc tttcgatttc tctttcgttc cggcttataa
     8701 tccccggcct ttgattattt ttttatcatt tctggaagca atatgagctc tatctccctg
     8761 atccaaccgg atcgcgacct gttctcctgg ccgcagtact gggccgcctg ttttggaccg
     8821 gcaccgtttt tgccgatgtc tcgtgaagag atggatcaac ttggctggga tagctgcgac
     8881 atcattttgg ttactggcga cgcgtatgtc gatcacccaa gcttcgggat ggcgatttgc
     8941 ggtcgtatgc tggaagcaca gggctttcgc gtcgggatca tcgcccagcc agactggagc
     9001 agcaaagacg atttcatgcg tctgggtaaa ccgaatctgt ttttcggtgt tactgctggc
     9061 aacatggatt cgatgatcaa ccgttatacc gccgatcgcc gtttacgtca tgacgatgcc
     9121 tacacgccgg ataacgtcgc gggtaagcgc ccggatcgcg ccacactggt ttatacccag
     9181 cgttgtaaag aggcgtggaa agatgtaccg gtgatcctcg gcggtattga ggctagtctg
     9241 cgccgtaccg cacattatga ttactggtcc gataccgtgc gccgttccgt gctggtggat
     9301 tcgaaagccg acatgctgat gtttggtaac ggtgagcgtc cgctggtgga ggtggcgcac
     9361 cgtctggcga tgggcgagcc gattagtgaa atccgcgatg tgcgtaatac cgcgattatc
     9421 gtgaaagagg cgctgcctgg ctggagcggc gtggattcca cccgtcttga tacccctgga
     9481 aaaatcgacc caatcccgca tccgtatggt gaagatttgc cgtgcgcgga taacaaaccg
     9541 gtggcaccga aaaagcagga agccaaagcc gtaaccgtgc agccaccgcg cccgaaaccg
     9601 tgggaaaaaa cctacgtgtt gctgccttct ttcgagaaag tgaagggcga taaagtgctg
     9661 tacgcccatg cttcgcgcat tctgcaccac gaaaccaacc caggctgcgc ccgcgcattg
     9721 atgcaaaaac acggtgaccg ctatgtgtgg atcaacccgc ctgctatccc gctttctacc
     9781 gaagagatgg acagcgtttt tgcgctgccg tacaagcgcg tgccacatcc ggcctatggc
     9841 aatgcccgta ttccggctta cgaaatgatc cgtttttcgg tcaacattat gcgcggctgc
     9901 tttggcggct gttctttctg ttctattacc gagcacgaag ggcgcattat tcagagccgt
     9961 tcggaagatt cgatcattaa tgagatcgaa gcgatccgcg acaccgttcc aggttttacg
    10021 ggcgtgattt ccgatctcgg tgggccaact gccaacatgt atatgttgcg ctgcaaatcg
    10081 ccacgcgctg aacaaacttg tcgccgtttg tcgtgcgttt atccggatat ttgtccgcac
    10141 atggacacta accacgaacc gacgatcaac ctctatcgcc gtgcgcgtga tctgaaaggc
    10201 attaaaaaaa tcctcatcgc ctcaggcgta cgttatgaca tcgccgtgga agatccgcgc
    10261 tatatcaaag agttggcgac ccatcacgtc ggcggttatc tgaagattgc cccggaacat
    10321 accgaagaag ggccgttatc gaagatgatg aagccgggca tgggcagcta tgaccgcttt
    10381 aaagagctgt tcgatactta ctcaaaacag gcaggtaaag agcagtatct gatcccctat
    10441 ttcatctccg cgcaccccgg tacgcgtgat gaagatatgg tgaatctggc gctgtggctg
    10501 aaaaagcatc gtttccgtct cgaccaggta cagaacttct acccatcgcc gctggcgaac
    10561 tcaaccacca tgtattacac cgggaaaaac ccgctggcga agattggtta taagagcgaa
    10621 gacgtcttcg taccgaaggg cgacaaacag cgtcgtttgc ataaagcgtt gttgcgttac
    10681 cacgatccgg caaactggcc gttaatccgc caggcgctgg aagcgatggg caaaaagcat
    10741 ctgattggca gccgtcgcga ttgcttagtg cctgcgccaa ccattgaaga gatgcgtgaa
    10801 gctcgtcgcc agaaccgcaa tacccgtccg gcgttgacga aacatacgcc gatggcgacc
    10861 cagcgtcaga cgcctgctac ggcaaaaaaa gcgtcgtcta cgcaatctcg tccggtgaat
    10921 gctggtgcga agaaacgccc taaagcggcg gttggacgtt aaaaaaaatc cccggcaact
    10981 gacatgctgc cggggatttt tttatcattc tgcgtacata cgcaagcagc gcaggcaatc
    11041 aaaatagtcc tgagcatcaa gacgcgtatt ggctctgtta gcttcggatt tggcagcata
    11101 gatttgtggg cgtagtgctt tcatatcctg ctcaagccgg gcgattttgg catttaatgt
    11161 ttgcacattg ctcgccagct gattgacctt ttgctcattg acgcagccac tgagcaagca
    11221 ggttgccaga acaacagctc ccacggtgaa aatcgttttc atatagtgtt cccttttatt
    11281 tattgttccg gctaattgtt atttaattca gctcattgct ttaatctggc ggcggttttt
    11341 gcctcaatct gcttaagtag gctgccgctg ttcgggcgga taaaatcagc gatttcccac
    11401 ttgccttcgc gccgctgtaa aaccagttct tgctcttcgt tgttgccatt ggtcagcgta
    11461 aaacgaacat gaatgcgggc ggaatcggcg gttttctcca ggactcgaat ttcatcaacg
    11521 cgtgaaaata gcgactcttc gccaaaacag atcggattgt cgtaaataaa ttgccgttct
    11581 atttgcatat cttcatcggg attattggcc gtcttgaagg tggttttata gaaggcatta
    11641 accgcctggc ggtaagagtc ggaaaccacc cacgtccacg gccagctata atcagcaata
    11701 tgttcaaaga gttcggcaac aaaggcttcc ggctgttctt tttgcaacga agctaaagcg
    11761 gccagcgttt tttcattctc gctattaact gcttgtaaga cgctgccatg attgctgaca
    11821 atatcgtcaa tgacccaacg accattttcc gccaccattt tcagtgtctg cgtggtcttt
    11881 tctttatcat ctttaaagat acgaaagcgc acaacggcat cggcatgatc ggcgtcagtt
    11941 tgggttatcg caacgctttc tagcaccaga tcgccaaaat cctgacaatc acaaaccgga
    12001 tcataatcca gccagccaat attgcccggc agcgtaagat tgtcgttcag ggtaagcgcc
    12061 tgttgaatac gcgcagaagt tatcgcccgc tctccggttt caccaaaata aggggcagtg
    12121 gcatctgatt tatagttctg ataaatatgc cggactgttt gttctacggt cagtgagggg
    12181 ggctgtgcgt gtacggtaaa acttgccagg gctgctaaaa acagaagtat tattttcatc
    12241 tgtttttcac ctctccgttg cgtttatccc ggatatagat gagttgttct tttaagagct
    12301 tccggctctg catgatgatg tccttatatt tggcattcct gaacaaggaa gagtaacaac
    12361 gggcgggacg cgaggggaat aaatgatttc tgaaaagtcc ggtagcggaa cattaccgcc
    12421 accgggagaa tttgcatgtt agccgccgaa ctggtcagga tcgggaccga gacgcttgcc
    12481 ctgatcgagt tttgcaattt cgccgagttc gtctttgtcg agacggaaat cccagacatc
    12541 aaagttttcg gcaatacgtg aaggtgtgac cgatttcggg atcaccacca ggccgctatc
    12601 cagatgccag cggataacaa tctgcgccgg ggttttgccg tatttatctg ccagatcgcg
    12661 aatgactttc tgatcgaaaa cgcctttccc tccttgcgct aatgggctcc aggattcggt
    12721 ctggattttg tgtgtcgcgt tccaggcgtg tagctggcgt tgttgcatca gcggatgaag
    12781 ttcgatctgg tttatcacag gcgtcacgcc agtttcatca atcaggcgtt gcaggtgatg
    12841 gatctggaag ttgcacacgc cgatgctttt gattaatccc tctttttgca attcgatcat
    12901 gcctttccat gcttcgacat aatggtcgat agcgggaacg ggtcagtgca ttaagtagag
    12961 gtcgatataa tcaagctgga gttttttcag gctgtcgagc agggcttcgc gggggcgctt
    13021 gtggtcgtcg ttccacagct tagtggtgat gaacagttct tctctgttga ctgaggcatt
    13081 tttcagggct ttgccgacac cttcttcgtt cttgtaggcc gcggcggtat caatcgagcg
    13141 ataacccact tctaacgctt tttgaatggc ggtgattact tcctcattac ttgcttgcca
    13201 gacgcccagt cccaactggg gcatgacatt gccatcctgt agcttaataa cggttggatt
    13261 agccatacgt tcctccttta tatgaactca ccggagcagg ctccggtgag gtgttgtgaa
    13321 cttaagtctg gacgaaatgc ccgaaaacga aagtttgagg cgtaaaaagc ttagcgggcg
    13381 gcttcgtata tacggcggct gacatccagc gtaatgtcat gattttcgcc cagttgggtc
    13441 atgccgtgct cttccagttt tttcagcaaa gccgggatgg agctgccgtc cagaccgtag
    13501 tcggagaggt gggtcggcac gcctaattgc tcaaagaaat tgcgggttgc ggcaatcgcg
    13561 gcgtcaatac gctcatcgtc ggaaccttca gtgatgttcc agacgcgttc agcatattgc
    13621 agcagcttag cgcgcttggt ctcgcgtttt tcattccaca gtgcaggcag gacgatagcc
    13681 agtgtttgcg cgtgatccag accgtgcatc gcagtcagtt cgtggcccag catatgcgtt
    13741 gcccagtcct gcggtacgcc agcgccaatc aaaccgttca gcgcctgagt cgccgcccac
    13801 atgacgttgg cgcgcacatc gtagttttct ggctctttca gggctttcgg accatcttcg
    13861 attagcgtca gcaaaatgcc ttctgcgaaa cggtcctgaa ttttggcatc aaccggtttg
    13921 gtaacatact gttccacggt gtgtacaaag gcgtccacta cgccgttagc cacctgacgc
    13981 ggcggcaggg tgtaggtata aaccggatcg agcacggcaa ataccggctg aacatgggca
    14041 gaatggaacg cctgcttgtc gcctgtggtt ttacgggaga tcaccgcgcc tgcgttggat
    14101 tctgaaccgg ttgctggcag cgtcagcaca cagcccatcg ggatggcgct tttaatctct
    14161 ttaccgcccg tttgcagaat gtgccacgga tcgatatttt ccggatagtt agccgctgcg
    14221 gcgataaatt tggtgccgtc cagtacagaa ccgccgccaa ccgccagcag gaaagtcact
    14281 ttctgttcgc gaaccagttt cacggcgttc atcagcgttt cataagccgg gtttggctca
    14341 ataccgccaa attccagcac gtccatgcct ttcagggcat ccagaacttg atcgagaacg
    14401 ccggtttttt tcacgctgcc gccgccgtag gtaatcaata cgcgagcatc gtgaggaatt
    14461 tgttcgcgta aaccagcgat tgcgccttta ccaaacagaa tgcgggttgg ggtgtgcaga
    14521 ttaaagttgt tcattacttg ctccctttgc tgggccaata tgagggcaga gaacgatctg
    14581 cctgatgttt ttcattgtga tcgccagcgc cctggctctc aatgctcatt tctgccaatg
    14641 tcttgcctat ttctccagag tgctggagaa atgctacaaa attgcgcaca atcaaattgc
    14701 cgcattattc c
//
LOCUS       DAZGVQ010000121        14678 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000121 DAZGVQ010000000
VERSION     DAZGVQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.122"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(210..286)
                     /locus_tag="ACHLAG_003701"
     tRNA            complement(210..286)
                     /locus_tag="ACHLAG_003701"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(250..252),aa:Arg,seq:acg)
     gene            complement(350..426)
                     /locus_tag="ACHLAG_003702"
     tRNA            complement(350..426)
                     /locus_tag="ACHLAG_003702"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(390..392),aa:Arg,seq:acg)
     gene            complement(624..700)
                     /locus_tag="ACHLAG_003703"
     tRNA            complement(624..700)
                     /locus_tag="ACHLAG_003703"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(664..666),aa:Arg,seq:acg)
     gene            complement(704..796)
                     /locus_tag="ACHLAG_003704"
     tRNA            complement(704..796)
                     /locus_tag="ACHLAG_003704"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(760..762),aa:Ser,seq:gct)
     gene            complement(1112..1297)
                     /gene="csrA"
                     /locus_tag="ACHLAG_003705"
     CDS             complement(1112..1297)
                     /gene="csrA"
                     /locus_tag="ACHLAG_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFP0656851.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1532..4162)
                     /gene="alaS"
                     /locus_tag="ACHLAG_003706"
     CDS             complement(1532..4162)
                     /gene="alaS"
                     /locus_tag="ACHLAG_003706"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFP0656852.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(4290..4790)
                     /gene="recX"
                     /locus_tag="ACHLAG_003707"
     CDS             complement(4290..4790)
                     /gene="recX"
                     /locus_tag="ACHLAG_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFP0656853.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(5033..6094)
                     /gene="recA"
                     /locus_tag="ACHLAG_003708"
     CDS             complement(5033..6094)
                     /gene="recA"
                     /locus_tag="ACHLAG_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP0656854.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(6174..6671)
                     /gene="pncC"
                     /locus_tag="ACHLAG_003709"
     CDS             complement(6174..6671)
                     /gene="pncC"
                     /locus_tag="ACHLAG_003709"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFP0656855.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6816..7901)
                     /gene="mltB"
                     /locus_tag="ACHLAG_003710"
     CDS             complement(6816..7901)
                     /gene="mltB"
                     /locus_tag="ACHLAG_003710"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFP0656856.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            8157..8720
                     /gene="srlA"
                     /locus_tag="ACHLAG_003711"
                     /gene_synonym="gutA"
     CDS             8157..8720
                     /gene="srlA"
                     /locus_tag="ACHLAG_003711"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFP0656857.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8717..9676
                     /gene="srlE"
                     /locus_tag="ACHLAG_003712"
                     /gene_synonym="gutE"
     CDS             8717..9676
                     /gene="srlE"
                     /locus_tag="ACHLAG_003712"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFP0656858.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9687..10058
                     /gene="srlB"
                     /locus_tag="ACHLAG_003713"
                     /gene_synonym="gutB"
     CDS             9687..10058
                     /gene="srlB"
                     /locus_tag="ACHLAG_003713"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP0656859.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            10062..10841
                     /gene="srlD"
                     /locus_tag="ACHLAG_003714"
     CDS             10062..10841
                     /gene="srlD"
                     /locus_tag="ACHLAG_003714"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFP0656860.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10946..11305
                     /gene="gutM"
                     /locus_tag="ACHLAG_003715"
     CDS             10946..11305
                     /gene="gutM"
                     /locus_tag="ACHLAG_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFP0656861.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            11372..12145
                     /gene="srlR"
                     /locus_tag="ACHLAG_003716"
                     /gene_synonym="gutR"
     CDS             11372..12145
                     /gene="srlR"
                     /locus_tag="ACHLAG_003716"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFP0656862.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            12138..13103
                     /gene="gutQ"
                     /locus_tag="ACHLAG_003717"
     CDS             12138..13103
                     /gene="gutQ"
                     /locus_tag="ACHLAG_003717"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFP0656863.1"
                     /translation="MSEALQNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(13100..14614)
                     /gene="norR"
                     /locus_tag="ACHLAG_003718"
     CDS             complement(13100..14614)
                     /gene="norR"
                     /locus_tag="ACHLAG_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFP0656864.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLSRATRNGDEVILEAQHFAFPEVTLPTPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
BASE COUNT         3410 a         4056 c         3671 g         3541 t
ORIGIN      
        1 gctacggatg catcgggaaa atttatttta ctgctgatat tgatatcgct actaaagcca
       61 tatcaagtaa gagatggtgc atccgggagg attactcgcc ttgcggctcg cccttcgggc
      121 cgttgctaaa gcaacgttat catccctggt gcctgcggtt aattccgcta cctcttcgat
      181 accttcattg ctgaagattt cggagaatat ggtgcatccg ggaggattcg aacctccgac
      241 cgctcggttc gtagccgagt actctatcca gctgagctac ggatgcatcg ggaaaattta
      301 ttttactgct gatattgata ccgctactaa agccatatca agtaagagat ggtgcatccg
      361 ggaggattcg aacctccgac cgctcggttc gtagccgagt actctatcca gctgagctac
      421 ggatgcatcg ggaaacttac tttactgcag attttttgat accgctacta aagccgtatc
      481 aagtaagaga tggtgcatcc gggaggatta ctcgccttgc ggctcgccct tcgggccgtt
      541 gctaaagcaa cgttatcctc cctggtgcct gcggttatac cgccacctct tccatacctt
      601 catcgctgaa agtacgtaga atatggtgca tccgggagga ttcgaacctc cgaccgctcg
      661 gttcgtagcc gagtactcta tccagctgag ctacggatgc aaatggcggt gaggcgggga
      721 ttcgaacccc ggatgcagct tttgaccgca tactccctta gcaggggagc gccttcagcc
      781 tctcggccac ctcaccacac gcctcttacg agtgcttcga agaacttgtt tatcgctcat
      841 cgtcgctgcg tggcgcacat attactttct gagacttata agtcaaacaa tttttcctac
      901 acttttatcg tttgcacact tcacgttcaa ttagtctgta aaaacgtcaa aaagagtgtt
      961 ttatcaacag aaaaatggag gtctgacaga tagtagtaat gcaaaaaaat ggagacttaa
     1021 gttgaatgaa cgggagtaaa gcgaaaagac tatagagtga aggagaaatt ttgagggtgc
     1081 gtctcaccga taaagatgag acgcggaaag attagtaact ggactgctgg gatttttcag
     1141 cctggatacg ctggtagatc tcttcacggt gaacagaaac ttccttcggg gcatttacgc
     1201 caatacgtac ctggttgccc tttaccccta aaactgtcac ggtgacctca tccccaatca
     1261 tgagggtctc accaactcga cgagtcagaa tcagcattct ttgctccttg aaagattaaa
     1321 agagtcgggt ctctctgtat cccggcatta tccatcatat aacgccaaaa agtaagcaat
     1381 gacaaacaca ttacatgtaa gcagtcatgg cattacattc tgttaaacct aagtttagcc
     1441 gatatacaca acttcaacct gactttatcg ttgtcgatag cgttaatgcg aatgccgtga
     1501 agcgagtcca cggcattgcc tgacgcctat attattgcaa tttcgcgctg acccagcctt
     1561 tcacactggc taacgctgca ggtaaggccg cagcatccgt accaccggct tgcgccatgt
     1621 caggacgtcc accacccttg ccgcccacct gctgagcgac cataccaatc agttcccctg
     1681 ctttcacacg atctgtgacg tccttagata cgcctgcaat cagagaaacc ttaccttcgg
     1741 ctaccgttgc cagcacgata attgtcgacc ccagctgatt ttttaaatcg tcaaccatgg
     1801 tacgcaacat tttcggctca acaccgctaa gctcgctaac caacagctta acaccattaa
     1861 catcaattgc cttactggaa agatttgcgc tctcctgtgc ggcagcttgt tctttaagct
     1921 gttgtagttc tttttctaac tgacgcgtac gttccagtac tgagcgcact ttatccgcca
     1981 gattattgct atcgcctttc agcagatgcg cgacttcgct taagcgatcg ctgtctgcat
     2041 gaacggtggt gatagcgcct tctccggtta ccgcttcgat acgacgaacg cctgcagcag
     2101 tacccgattc agagatgatg cggaacagac caatatcacc agtgcggctg gcgtgagtac
     2161 cgccacacag ctcggtggag aaatcgccca tgctcagcac gcgtacacgc tcatcatact
     2221 tctcgccgaa cagcgccatc gcacctttcg ctttcgccgc ttcgagatcc atgatgttgg
     2281 tttcgatcgg caaattgcga cgaatctgtg cgttcaccag gtcttcgacc gcacgaatct
     2341 cttccggttt catcgcttcg ttgtgtgaga agtcgaagcg cagcaccttg tcgttaacca
     2401 gtgaaccttt ctgcgataca tgagtcccca gaacctggcg cagcgcagcg tgcatcaggt
     2461 gcgttgcgga gtgattcaga cgaatacggg cgcgacgagc ctcatcaaca tcagcctgca
     2521 cagcgtcgcc cactttcaga gaacccgcag caagtttacc gatgtgacca atcgcctggc
     2581 cgtatttctg cgtatcttcc accgcgaagg agaagttagc gcctttcagt tcgcctttat
     2641 caccaacctg accgccggat tccgcataga atggtgtttg atccagcacg accacagctt
     2701 cctggcctgc attgatggca tcaaccgctt taccatcaac aaacagcgcg gtcactttgc
     2761 cgttcagttc cagatggtca tagcctttaa attcagatgc actatcaaca cggatcattg
     2821 cgttgtaatc ggcaccaaag ccgctggctt cgcgcgcgcg acgacgctgc tcttccattg
     2881 ctgcttcaaa accagcttcg tcaactttga tgttgcgctc acgacaaaca tcagccgtca
     2941 ggtcaaccgg gaagccatag gtgtcgtaca gacggaaagc cgtttcacca tccagcgtat
     3001 caccagaaag ttttgccagc tcttcatcca gcaacgccag accgcgctcc agagtacggg
     3061 caaactgctc ttcttcagtc ttcagcacct gctcaacctg cgcctgctgg cgtttcaggt
     3121 cttcacccgc agagcccata acgtcgatca gcggaccaac cagtttgtag aagaaggttt
     3181 ctttcgcgcc gagcatattg ccgtgacgca ctgcgcgacg aatgatacga cgcagtacat
     3241 aaccacggtt ttcattggac ggcattacgc catccgcgat caggaacgca caagaacgaa
     3301 tgtggtcagc gattacgcgc agcgatttat tgctcagatc ggtcgcgcca gtgactttcg
     3361 ctaccgcctg gatcaacgtg cggaacaggt cgatgtcata gttagagtta acgtgttgca
     3421 gcaccgcagc aatacgctcc agacccatac cggtatctac agacggcttc ggcagcggtt
     3481 ccatcgtgcc atctgcctgg cggttgaact gcatgaagac gatgttccag atctcaatgt
     3541 agcggtcgcc gtcttcttcc ggacttcccg gagggccacc ccaaatgtgg tcgccgtgat
     3601 cgtagaagat ttcggtgcac gggccgcacg gaccagtgtc acccatctgc cagaagttgt
     3661 cagatgcgta tggcgcacct ttgttatcgc cgatgcgaat aatacgttcg cgcgggatcc
     3721 cgacttcttt ttcccagatt tcgtaggctt cgtcgtcgct ttcatagacg gtaacccaca
     3781 gacgctcttt cggcagggca aaccattttt cgctggtcag cagttcccat gcaaactgaa
     3841 tggcatcgtg tttgaaatag tcgccgaagc tgaagttgcc cagcatttcg aagaaggtat
     3901 ggtgacgcgc ggtgtaaccg acgttttcca ggtcgttgtg tttaccaccc gcacgcacgc
     3961 agcgttggga agtggtagcg cgggaataat tacgcttgtc gagcccaagg aacacatcct
     4021 tgaactggtt catcccggcg ttggtaaaca acaaagttgg gtcgttatgg ggtaccaggg
     4081 agctgctggc aactacctga tgtcccttac tatggaaaaa gtcgagaaac gcctgacgga
     4141 tctcagcggt gctcttgctc ataattatcc tgaaatcaag ctaacgaaat atcgccacca
     4201 gctccagcgt gtcttaaccg ccgggctggt aactgaaaag tgggaataag ataagttttc
     4261 ttgactggga agtaaaatac cgtatgcgtt cagtcggcaa aatttcgcca aatatcctgg
     4321 atatcttcca tcagatagcc acgatagagc agaaaacgct ggatcttaac tttttctgaa
     4381 aagacagttg gcaaaggttc accatatttt cgcgtcgcct gatcgcgcgc cagtgcgcac
     4441 cagtcgatgt cacattcacg catcgctttt tctgtcgctt cgcgggaaat acctttctga
     4501 ttcagttcct gacgaatacg tgcaggtcca taacctttgc ggctacggct ggcgataaag
     4561 cgcgcaacaa atcggctgtc atcgagatag ccatgttcat ggcaccaggc aataacgcgc
     4621 tcgtaatctt ctgccgtagc atcaatctct tctgggccat ttttgcccat aatcggtgcc
     4681 gcgagtttac gtcgcagttc ttgctcactg tgatcgcgca ccgccagaat gcgtaccgca
     4741 cgatccaaca ggcgagcata tgccgggcga cgggacgttg attctgtcat ggcatatcct
     4801 tacaacttaa aaaagcaaaa gggccgcaga tgcgacccag gttttgctga taaacagatt
     4861 taatagtcaa tgagtgtgca gaatgttccc gcaggcccaa ttcgttagag gatcgaggtg
     4921 agagtaattt ttcacgccag tgctggcctg ttcccctcac cctaaccctc tccccaaagg
     4981 ggcgagggga ccgtccgtgc acacattatc tattatcatc aataacgaac gattaaaaat
     5041 cttcgttagt ttctgctacg ccttcgctgt catctacaga gaaatccggc gttgagttcg
     5101 ggttgctcag cagcaactca cgtactttct tctcgatctc tttcgcggtt tccgggttat
     5161 ctttcagcca ggcagttgca ttcgctttac cctgaccgat cttctcacct ttgtagctgt
     5221 accacgcgcc tgctttctcg atcagcttct cttttacgcc caggtcaacc agttcgccgt
     5281 agaagttgat accttcgccg tagaggatct gaaattcagc ctgtttaaac ggtgcagcga
     5341 ttttgttctt caccactttc acgcgggttt cgctacccac cacgttttcg ccctctttca
     5401 ccgcgccgat acgacggatg tcgagacgaa cagaggcgta gaatttcagc gcgttaccac
     5461 cggtagtggt ttccgggtta ccgaacatca caccaatttt catacggatc tggttgatga
     5521 agatcagcag cgtgttggac tgcttcaggt tacccgccag cttacgcatc gcctggctca
     5581 tcatacgtgc cgcaaggccc atgtgagagt cgccgatttc gccttcgatt tccgctttcg
     5641 gcgtcagtgc cgccacggag tcaacgacga taacgtctac tgcgccagaa cgcgccaggg
     5701 cgtcacagat ttccagtgcc tgctcgccgg tgtccggctg ggagcacagc aggttatcga
     5761 tatcgacgcc cagtttacgt gcgtagattg ggtccagcgc gtgttcagca tcgataaacg
     5821 cacaggtttt accttcacgc tgcgctgcgg cgatcacctg caacgtcagc gtggttttac
     5881 cggaagattc cggtccgtag atttcgacga tacggcccat cggtagacca cctgccccaa
     5941 gcgcgatatc cagtgaaagc gaaccggtag agatggtttc cacatccatg gaacggtctt
     6001 cacccaggcg catgatggag cctttaccaa attgtttctc aatctggccc agtgctgccg
     6061 ccaacgcttt ctgtttgttt tcgtcgatag ccatttttac tcctgtcatg ccgggtaata
     6121 ccggatagtc aatatgttct gttgaagcaa ttatactgta tgctcataca gtatcaagtg
     6181 ttttgtagaa attgttgcca caaggtctgc aatgcatacg cagtagcctg acgacgcacc
     6241 gcatcacggt cgccgctgaa gcattcccgc cgggtaatgc cttcaccgcg ggcagtggca
     6301 aaagcaaacc agacggtgcc gacaggcttc tcttcactgc cgccatccgg cccggcgata
     6361 ccactaatag acacggcata atcagcacga gccgctttca gtgcgcctat caccatttcc
     6421 accacgacgg gttcactcac cgcgccatgc tgcgccagcg tctcttcgcg tacgccgatc
     6481 atctgcgctt tggcttcgtt actgtaggtg acaaatccgc gttcaaacca ggcggagcta
     6541 ccggcaatat cggtaatcac tttcgctacc caaccaccgg tacaagactc ggcagttgtt
     6601 acggttgcgc cacgggcttt cagcgcctgc ccaacctgtt cacttaactg catcagttca
     6661 ctgtcagtca tcacaacctc tgtcagtcaa aaatttatgc cggacaagat agcactttcg
     6721 tcaggaagat ggtgttggcg ttgcaatttt acgaggagga ttcagaaaaa agctgattag
     6781 ccagagggaa gctcacgccc ccctcttgca aatagttact gcacgcgcgc cagcgccacg
     6841 gcttgtccta actgccagac cgccattgcg taatgggtgc tgtggttata acgggtaatg
     6901 gtgtagaagt tcggcagacc gtaccagtac tggtaaccgg taccgacatc cagacgcagc
     6961 aggctggctt gttgatggtt gcccagcggc tgctgtggcg ttaaacctgc cgtggcaagc
     7021 tgcgaaatgc tgtacctggt tttgaagcca tttggcaagc ctggagcctg accgtttgcc
     7081 attaccgcga cctgatcgcc tttcacccag ccgtgcgctt tgaaatagtt cgccacgcta
     7141 ccgatcgcat caaccggatc ccacaggttg atatgcccgt cgccgctgaa atctaccgca
     7201 tattgtttgt aagacgacgg cataaactgt ccgtagccca tcgccccggc aaaggaacct
     7261 ttcagattga gcggatcgtc ctgctcgtcg cgcgccatca acaggaaggt ttccagctcg
     7321 ccagagaaat actccgcgcg gcgtgggtag ttaaatgaca gcgttgccag cgcatcgagg
     7381 atgcgagttt tccccatcac gcgcccccag cgggtttcaa cgccgataat cccgacgata
     7441 atttccggcg gtacgccata cacctgccac gcgcgattca acgcatcttc atactgattc
     7501 cagaaaacca caccgttctg cacgttgtcc ggcgtaataa attttttgcg ataacggagc
     7561 catgcgccgt tcggacctga tggcggtttc accgatgtgg ttggtgcctg gttatccatc
     7621 agccgcagta ccgaatccag acgtttcgcc tgggagagaa tttcctgcaa ctgctgacga
     7681 tcgaaaccgt gtttgttcac cattttgtcg atgaactgct gggcattcgg gttattagcg
     7741 aaatcgccgc ccatctgcat cacattgtgc tgcggctcaa gcaggaagcc gccagacggc
     7801 gttccggtgg tcgtttcagt ctcagtaggt tttggcttgc tgctacaggc ggcaagcaac
     7861 acaaaaaggg gaagcaatgt tacataacga cgcttgaaca tgaggggtcc atttaacaga
     7921 ttcaaccagg ggcaagtatg gtaaagcatc acgccccaca caaggaagcg gtagtcactg
     7981 cccgatacgg actttacata actcaactca ttcccctcgc tatcctttta ctcaaacttt
     8041 caaattaaaa tatttatctt tcattttgag atcaaaataa cacttttaaa tctttcaatc
     8101 tgattagatt aggttgccgt ttggtaataa aacaataaat cctgaaggag agaacaatga
     8161 tagaaaccat tactcatggt gcagagtggt ttatcgggct gttccaaaag ggcggagagg
     8221 tgtttaccgg gatggtgacc ggcattcttc cgctgttgat tagcctgctg gttatcatga
     8281 acgcactgat taattttatc ggtcagcatc gtattgaacg ttttgctcaa cgttgcgccg
     8341 gtaaccctgt ttcccgttac ctactgttac cgtgcattgg cacgtttgtc ttttgcaatc
     8401 cgatgacctt aagcctgggt cgctttatgc cggaaaagta caaacccagc tactacgcgg
     8461 cggcctctta tagctgccac tcaatgaatg gcctcttccc ccatatcaac cctggcgaac
     8521 tgtttgttta tcttggcatt gccagcggtc tgacaacgct gaacctgcca cttggcccac
     8581 tggcggtgag ttatctgctg gttggtctgg tcaccaattt cttccgcggc tgggtgaccg
     8641 atctgaccac cgccattttt gagaaaaaga tgggcattca acttgaacaa aaagttcacc
     8701 tggcaggagc aacatcatga cgcatattcg gatcgaaaaa ggaacgggtg gctggggcgg
     8761 cccgcttgag ctgaaagcca caccgggaaa aaaaatcgtc tatatcaccg ccggtacccg
     8821 gcctgcgatt gttgacaaac tggcacagct tactggctgg caggctattg acggatttaa
     8881 agaaggtgaa cccgcggagg cggaaattgg tgtcgcggta atcgactgtg gcggcacatt
     8941 acgctgcggc atctatccga aacgacgtat tcccaccatt aatatccact cgacgggcaa
     9001 gtccggtccg ctggcgcagt acattgtgga agatatttat gtctctggcg taaaagaaga
     9061 aaacatcact gtagtgggtg atgcgacacc acaaccctct tccgtgggcc gtgactatga
     9121 caccagtaag aaaatcaccg aacaaagcga tggtttactg gcgaaggtgg gaatgggcat
     9181 ggggtccgcc gttgcggtgc tgtttcaatc tggtcgtgac accatcgaca ctgtattaaa
     9241 aaccattctg ccgtttatgg cattcgtctc ggcgctcatt ggcatcatta tggcttctgg
     9301 ccttggtgac tggattgccc acggtcttgc tccgctggcg agccatccac tgggtctggt
     9361 catgctggcg ctcatctgct ccttcccact gctttcacct ttcctcggcc caggcgcagt
     9421 tatcgcacag gttatcggcg tattgattgg cgtgcagatt ggtctcggca atattccgcc
     9481 gcatctggct ttacctgcac tgtttgccat caacgcgcag gcggcctgcg acttcatccc
     9541 ggtcggtttg tcgctggcgg aagcccgtca ggacacggtt cgcgtcggtg tcccttctgt
     9601 actggtgagc cgctttttaa ccggcgcgcc gactgtactg atcgcctggt ttgtctccgg
     9661 ttttatctat caatagaggc tgaaacatga ccgttattta tcagaccacc atcacccgta
     9721 tcggcgcgag tgccactgac gccctcagcg accagatgct catcaccttt cgtgaaggcg
     9781 cgccagcgga cctcgaagag tattgcttca ttcattgcca cggcgagttg aaaggtgcac
     9841 tccatcccgg tttgcaattt tcactcgggc agcatcgcta tccggtgacc gctgttggca
     9901 gcgtggcgga agacaacctt cgcgaactgg gtcatgtcac cctgcgcttc gatggtttaa
     9961 acgaagcgga atttccgggc actgtccatg tggcaggccc tgtacccgac gatatcgcgc
    10021 cgggatcggt tttgaagttt gaatctgtta aggagtaaaa aatgaatcag gttgccgttg
    10081 tcatcggtgg tgggcaaacc ttaggcgcgt tcctgtgcca cggtctggct gccgaggggt
    10141 atcgcgtcgc ggttgtcgat attcagagcg acaaagccgc aaatgtggca caagaaatta
    10201 acgccgaata tggtgaaggt atggcgtacg gttttggtgc tgacgccacc agcgagcaaa
    10261 gcgttctggc gctctctcgt ggggtagatg aaatctttgg tcgcgtggat ttgctggtct
    10321 atagcgccgg aatagcaaaa gcagccttta tcagcgactt ccagctcggc gattttgacc
    10381 gttcgctaca agtgaatctg gtgggttatt tcctgtgtgc ccgtgaattt tcgcgtttga
    10441 tgatccgcga cgggattcag ggtcgcatta ttcagatcaa ctcgaaatcc ggcaaagtgg
    10501 gtagcaaaca caactctggc tacagcgcag cgaaatttgg tggcgtcggg ctgactcaat
    10561 cactggcgct ggatctggcg gagtacggca ttacggtgca ttcactgatg ctcggtaacc
    10621 tgctgaaatc gccgatgttc cagtcactgt tgccacaata cgcgaccaag ctgggtatca
    10681 aaccggatca agtcgagcag tattacatcg acaaagtacc gctcaaacgc ggctgcgatt
    10741 atcaagatgt gctgaatatg ctgctgttct acgccagtcc taaggcgtcg tactgcaccg
    10801 gacagtcgat caatgtcacc ggcggtcagg tgatgttctg atcaacagcg gagatccgtt
    10861 aaggatctcc gtgagactat agaatgcctg atgcgctacg ctcatcaggc atacaggact
    10921 tccgccacta cattaaggaa aagttatggt atccgcactc atcaccgtcg ccgttatcgc
    10981 ctggtgtgcg caactggcct taggcggctg gcaaatttct cgttttaacc gtgccttcga
    11041 cacactatgc cagcaagggc gggttggcgt gggccgttcc agcgggcgct ttaaaccgcg
    11101 ggtcgtggtc gccatcgccc tggacgatca gcagcgcatc gtcgacacct tgtttatgaa
    11161 aggactgacc gtcttcgccc gaccgcaaaa aattcccgca attaccggta tgcatgcggg
    11221 tgatttacag cccgatgtga tctttcccca tgatccacta tcacagaatg ctctatcatt
    11281 ggcgcttaaa ctgaaacgtg gataatttcg ttgtgaatgt tacttgcttg cgaagttatc
    11341 attttgaaac ctaaatcagg taatcacgcc catgaaacct cgtcagcgtc aggccgccat
    11401 tctggagtat ctgcaaaagc agggtaaatg ctcggttgaa gaattggcgc aatactttga
    11461 caccacaggc acaaccattc gcaaagatct ggtcattctg gaacatgccg gaaccgtcat
    11521 tcgtacttat ggcggagtgg tgttgaataa agaggaatcc gatccgccta tcgatcataa
    11581 aacactcatc aacacccaca agaaagagct gattgcagaa gctgccgtta gttttatcca
    11641 tgatggcgat tcgatcattc ttgatgctgg cagtaccgtt ttgcagatgg ttcccctgct
    11701 ctcgcgcttt aataacatca cggtgatgac caacagcctg catatcgtca atgcgctatc
    11761 cgaactggat aacgaacaaa ctatcctgat gccaggcgga acgtttcgta aaaaatcggc
    11821 ctcatttcac ggacagctgg cagagaatgc cttcgaacat ttcagtttcg ataaattgtt
    11881 tatgggcacc gacggcatcg atctcaatgc gggcgtaacc accttcaacg aggtttatac
    11941 cgtcagtaag gcaatgtgca atgccgcgcg cgaagtgatt ttgatggcgg actcatcaaa
    12001 gtttggccgt aaaagcccca acgtagtttg cagtcttgaa agcgtcgata agctgattac
    12061 cgacgcaggt atcgatccgg cgtttcgtca ggcgctggaa gagaaaggga tcgatgtgat
    12121 cataaccgga gagagcaatg agtgaagcac tacagaacgc gggacgtcag acgttaatgc
    12181 tggagttgca ggaagcaagc cgtttaccgg aacgtctggg cgatgatttt gttcgcgccg
    12241 ccaatatcat cctgcactgc gaaggcaaag tggtggtttc gggaattggc aaatcgggcc
    12301 acattggtaa gaaaatcgcc gcaacacttg ccagtaccgg cacaccggct ttttttgtcc
    12361 atccggcaga agcactgcac ggcgatctgg ggatgattga aagccgcgat gtgatgctgt
    12421 ttatctctta ctccggtggc gcgaaggaac tggatctgat tattccgcgt ctggaagata
    12481 aatctatcgc gctgctggcg atgaccggca aaccgacgtc accgctgggc ctggcggcaa
    12541 aagcggtgct ggatatctcc gtagaacgcg aagcctgccc gatgcacctt gcgccgacct
    12601 ccagcaccgt caataccctg atgatgggtg acgcgctggc gatggcggtc atgcaggcgc
    12661 gcggatttaa tgaagaagat tttgcccgct cccacccagc cggggcactg ggcgctcgct
    12721 tgctgaataa agtgcatcat ctgatgcgcc gtgacgatgc catcccacag gtggcgttaa
    12781 ccgccagcgt gatggatgcg atgctggaac tcagccgcac cggtctgggg ctggtggcgg
    12841 tatgtgacgc tcaacaacag gtacaaggcg tctttaccga cggcgattta cgtcgctggc
    12901 tggttggcgg cggcgcactc accacgccag ttaatgaagc gatgacggtc ggcggcacca
    12961 cgttgcaatc gcaaagtcgc gccatcgacg ccaaagagat cctgatgaag cgcaaaatca
    13021 ctgccgcacc ggtggtagat gaaaacggca aactcaccgg cgcaattaac ctgcaggatt
    13081 tctatcaggc cgggattatt taatccttca ttcccagacg tttcgccagc cgatgcaggt
    13141 tggcgacgtc ggtttccagc atccgcgcgc aggcagccca gttgtgatga ttttgtgcca
    13201 gcgcctgacg aatagtttca cgctggaacg cttctgtcgc ttcacgcagg ttttgcttaa
    13261 caacgggcac cgccgccact tctggcgtcg gcaacgtcac ctcaggaaaa gcaaaatgtt
    13321 gcgcctcaag aatcacttca tcgccgttgc gggtggctct cgacagcact accgcccgat
    13381 gaatagcatg ttccagttcg cgcacgttcc ccggaaaacg atagtgttgc agtaaatttc
    13441 gcgctccggc acttaatacc acgcgggaga gccccagccg caaacgacac tgctcgcaga
    13501 aataccccgc cagcagaatg acatcatcgc cccgctcacg cagcggcggc accgaaagtg
    13561 gaaacacgct caggcgatga aacaagtcag cgcgaaatcg ccctgccagc acctcttcgc
    13621 gtaagtcgcg gttagtcgcc gccagcacgc gcacatcgac ccgcaaactg cggtcatcgc
    13681 caacgcgctg aatatcgcca tactgcaaca ccctcagcag cttggcctgc aatgccaacg
    13741 acaactcgcc gatctcatcc agaaacagag tgccgttatc cgccatttca aacttcccgc
    13801 tgcggttact gatagcgcca gtaaacgctc ctttcacatg cccgaacagt tcactttccg
    13861 ccacactttc cggcagtgcg gcacagttga gatagaccag cggattcacc gcccgtggcg
    13921 aggcttcatg aatcgctttc gccaccagct ccttaccggt tcccgtctca ccgctgatca
    13981 ggacgttgag atcggacgcc gccacaatct caatctcttt tttcagttgc gtcatgccag
    14041 gggacaagcc aatcatctgc gtctgtttca ccgcttcaaa cggcgtggca tcgcctggca
    14101 tcatattctg gctttccagt tgttcaatca gcaacgcatt gcttaacgct cccgccgcca
    14161 gcgcagcaat cagccgtagc tcttcgtcgc tgaaaacatc gaactgatcg ggctgcatcc
    14221 cgtcgagcgt cagtgcgccg atcaggtttt gcccggcaaa caatggcaga ccaacgcagg
    14281 cgtgaacctt cagactctcc tgcccaggaa tcaaaccgtc atagggatcg ggcaattcgc
    14341 tgtctgcggg aaagcgcacc acatccccgg cgcgggcaat cgcttccagc cgtggatgcc
    14401 cttccagcgc aaagcgtcta ccgagtacat cctttgccag accgtcgatg gcaagcggaa
    14461 taaactgccg cgaatcgtaa cgtagcaacg cagacgcatc gcactccagc acctgacgta
    14521 gcgtggtgat caggcgctga aaacgatcct gatgaccaat cccacgctgc aattcgatgg
    14581 cgatattcgc cagcacatca acggaaaaac tcatctttgc ctcactgtca atttgactat
    14641 agatattgtc atatcgacca tttgattgat agtcattt
//
LOCUS       DAZGVQ010000122        14597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000122 DAZGVQ010000000
VERSION     DAZGVQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.123"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..733)
                     /locus_tag="ACHLAG_003719"
     CDS             complement(<1..733)
                     /locus_tag="ACHLAG_003719"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP0656865.1"
                     /translation="MSELSYLEKLLDGAEVEWVALNKLATFLKGKSLPKEKITPDGNR
                     YCIHYGELFTHYGPIIDKVCSKTNQAINESILSEKNDVLMPTSDVTPRGLATASCIQE
                     SGVILGGDILIIRCSGVDGRYLSNFIINNKKKILQMVKGSTVYHLYAKDIGKLLIPIP
                     CPNNPEKSLAIQSEIVRILDKFTALTAELTAELSMRKKQYNYYRDQLLSFKEGEVEWK
                     ALGEIGEVRMCKRILKSQTSSEGEIP"
     gene            complement(730..2286)
                     /locus_tag="ACHLAG_003720"
     CDS             complement(730..2286)
                     /locus_tag="ACHLAG_003720"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFP0656866.1"
                     /translation="MTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFIS
                     ENFSSYIEAGDDSICYAKLDDSVITDDIKDDAIKTKGYFIYPSQLFCNVAAKANTNDR
                     LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVE
                     GLKLGDFHEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHV
                     NKIYDPAAGSGSLLLQAKKQFDDHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFD
                     IKLGNTLTEPHFRDEKPFDAIVSNPPYSVKWIGSDDPTLINDERFAPAGVLAPKSKAD
                     FAFVLHALNYLSAKGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFG
                     TTIAVNILVLSKHKTDTKVQFIDASELFKKETNNNILTDAHIEQIMQVFASKEDVAHL
                     AKSVAFETVVANDYNLSVSSYVEAKDTREIIDIAELNAELKTTVSKIDQLRKDIDAIV
                     AEIEGSEVQA"
     gene            2645..2896
                     /locus_tag="ACHLAG_003721"
     CDS             2645..2896
                     /locus_tag="ACHLAG_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFP0656867.1"
                     /translation="MSNWNIAAKSQEERNKVNVDLAARGVAYKERLNIPVIAEQVARE
                     QPENLRTYFMERLRHYRQLSLLLPKGNDPVYQKEDIAKK"
     gene            2995..3384
                     /locus_tag="ACHLAG_003722"
     CDS             2995..3384
                     /locus_tag="ACHLAG_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFP0656868.1"
                     /translation="MGFPSPAADYAESRISLDQQIIRHPSATYFMRAADSHHREGILQ
                     GALLVVDSSLTPVDGSLLVCALDGEYRVKRYRKYPRQHLEDLRTGKKEALPKDDDGCT
                     GSNAVFGVITHIINDARSGEFDDCPVM"
     gene            complement(3421..4521)
                     /gene="apbC"
                     /locus_tag="ACHLAG_003723"
     CDS             complement(3421..4521)
                     /gene="apbC"
                     /locus_tag="ACHLAG_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFP0656869.1"
                     /translation="MSESKSPDALRAMVAGTLANFQHPTLKHNLTTLKALHHVALMDD
                     TLHVELIMPFVWNKPFEDLKEQCSGDLLRITGAKAIDWKLSYNIATLKRVKNQPGING
                     VKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQRPT
                     SPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDYLVL
                     DMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVENMS
                     VHICSNCGHHEPIFGTGGAQKLAEKYNTQLLGQMPLHISLREDLDNGTPTVISRPDSE
                     FTAIYRDLADRVAAQMYWQGEVIPGEIAFRAV"
     gene            4679..6712
                     /gene="metG"
                     /locus_tag="ACHLAG_003724"
     CDS             4679..6712
                     /gene="metG"
                     /locus_tag="ACHLAG_003724"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP0656870.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYTRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKAPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDTT
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            6952..7173
                     /locus_tag="ACHLAG_003725"
     CDS             6952..7173
                     /locus_tag="ACHLAG_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656871.1"
                     /translation="MIASNELDWVSQIEYGCIVHAGLQDDDWKDELRRYGISEGAIAN
                     IQKVLDVGFDSVHFDCGAPVVEGLECWQW"
     gene            7210..7395
                     /locus_tag="ACHLAG_003726"
     CDS             7210..7395
                     /locus_tag="ACHLAG_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656872.1"
                     /translation="MLKEDSYLLKALEMRGVEPTVKNAHDIYLQFSPELKRFINQYGV
                     NDSSVKAMIDDELDHLI"
     gene            7823..8119
                     /locus_tag="ACHLAG_003727"
     CDS             7823..8119
                     /locus_tag="ACHLAG_003727"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656873.1"
                     /translation="MPFIIIVASLAWVIFIGIPHKDEVALEEAACPLVTTILKENNGS
                     TAPKCMKVTIEDKVTDKFYRATATLDNGNDINITLELTGDRNFYVRVPNVYLNN"
     gene            9684..10013
                     /locus_tag="ACHLAG_003728"
     CDS             9684..10013
                     /locus_tag="ACHLAG_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656874.1"
                     /translation="MFANIDINQIKKLTQKEFDQFYELEGWSSTLINSRWVLELMTRD
                     DAPALMISDLGEDADFMDMSEFCVDTYNRSQKYYFTCDSENDVISKVYLHLVQHWDVQ
                     EFLEVFA"
     gene            10167..10478
                     /locus_tag="ACHLAG_003729"
     CDS             10167..10478
                     /locus_tag="ACHLAG_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656875.1"
                     /translation="MKKSLVLGLDKDQKRKEKPALVAQLTLLDIVANGTSIRLFRETA
                     VSFDKNTFTRYVMNVRRQRGKGWVAFQRMWPEHQLELALMEVNRVAQQEIQRASVMAI
                     A"
     gene            10605..11000
                     /locus_tag="ACHLAG_003730"
     CDS             10605..11000
                     /locus_tag="ACHLAG_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656876.1"
                     /translation="MTVKREKLTVDVYYASETAEGKNVAKITVVTYNTETGAEVQAST
                     IVRKGDASDGEYATQYQSILDATDPLLLKIENYFRQVDEEVFETMMNMVNTVFASNLN
                     TNTTWIGQYGLRITSGIPADTLIPESVFA"
     gene            11118..11492
                     /locus_tag="ACHLAG_003731"
     CDS             11118..11492
                     /locus_tag="ACHLAG_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656877.1"
                     /translation="MGLDIYIETQPKNDLNNEASRKQVAYFRKFNALVGWMERNVGEV
                     ENCELLELTMNDICFLKAHLMHINESNCEEYLPTREGFFFGSQEYDEGYWHDVGELKE
                     LVEDLIKNHDFHNNRLTFCAWW"
     gene            11828..12109
                     /locus_tag="ACHLAG_003732"
     CDS             11828..12109
                     /locus_tag="ACHLAG_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFP0656878.1"
                     /translation="MTDFTITPKAQNVFLESWLDLPETEQQEMDHVEYDEQVSTRFFH
                     FEGCVYDIADFMRDDRFPEWHASYPLNAFAMLMIRVDDSGDTIDIGLLH"
     gene            12314..12796
                     /locus_tag="ACHLAG_003733"
     CDS             12314..12796
                     /locus_tag="ACHLAG_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001711142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656879.1"
                     /translation="MNFIATVNAPAHGNIAVTFSDIEKRVLGAWRDNETVELSAQEKC
                     IIARDIIGNRRYSRVFEKAYVVNSGFGTFVFPVRSGRFCQSKLIEFATQISVWIKTQS
                     SFKFSDDEAVSQGMRIANNAIKCKNITYTAGVDTWKLFCANFMLNVYASNRIHILDGV
                     "
     gene            complement(13404..13634)
                     /locus_tag="ACHLAG_003734"
     CDS             complement(13404..13634)
                     /locus_tag="ACHLAG_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656880.1"
                     /translation="MFVLIAGVNVHNEYYVNRIAGIAGYAGRVVELIDETTRKIDLLS
                     DQERKKADVNDADLFLMLKAFVEMGFKISLHK"
     gene            complement(13656..13859)
                     /locus_tag="ACHLAG_003735"
     CDS             complement(13656..13859)
                     /locus_tag="ACHLAG_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656881.1"
                     /translation="MVSYDKIRAEYRANYRAYKLELIDDLIAQRDQLNFTFSDLLNSK
                     RDCKRKREYLRLSALIGKLQNSI"
     gene            complement(13908..14558)
                     /locus_tag="ACHLAG_003736"
     CDS             complement(13908..14558)
                     /locus_tag="ACHLAG_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656882.1"
                     /translation="MANIIISKKSIIEAAAIVSDELREKADLATQTYNEHYKNGTHTK
                     ADKANMQAATTKLAYFINNVVNAVEDEKLCSVFYYAIKASKQAPEVFFRDAMTNSYSL
                     EKLVYLVKSIKAGKCVYSIADMSGSRVFALIDMINDEIDTFTNGAVFDLMNEAKKACE
                     IKLDAGYTQANQLINLCERLGLVEKVKGAGSAKAGTQQYRFIKNDFYNYLADAFKA"
BASE COUNT         4115 a         3354 c         3296 g         3832 t
ORIGIN      
        1 atggaatctc tccctcagaa gatgtttgtg attttaggat tcgtttacac atacgaactt
       61 caccaatttc ccccaaagct ttccactcaa cctcaccctc tttaaaactc aacaactggt
      121 cgcgatagta gttgtattgt tttttacgca tgctaagctc agcggtaagc tcagcggtaa
      181 gtgcagtaaa tttatccaga atcctgacga tttcagactg gatagccagc gacttttccg
      241 ggttgttggg gcaagggatg gggataagta atttacctat atctttagca tacaaatgat
      301 aaacagtaga acctttaacc atttgcaata tctttttttt gttatttatt atgaaattac
      361 ttagatatct accatcaaca ccagagcatc taataatcaa aatatcgccg cctaaaataa
      421 cgccactttc ttgaatgcaa ctagctgtag ccaaacctct tggtgtaacg tctgacgttg
      481 gcattagaac atcatttttt tcagatagaa ttgactcatt gattgcttgg tttgtttttg
      541 agcatacttt gtcaattatg ggaccataat gagtgaaaag ctcaccgtaa tgaatacaat
      601 atctattgcc atctggagtt attttttctt taggtaaaga cttccctttt aaaaaagttg
      661 ctaatttatt taatgctacc cactcaacct cagccccatc cagcagtttt tccagataac
      721 tcagctcgct catgcctgta cctcgctacc ttcaatctca gcaacaatcg catcaatatc
      781 tttacgcaac tggtcgattt tgctgaccgt ggttttcagc tcagcattca gctcagcgat
      841 atcgataatt tcgcgagtat ctttcgcttc cacatagctg ctcaccgaca ggttatagtc
      901 attcgcgaca acagtctcaa acgcaacaga tttcgccaga tgagcaacat cttccttgct
      961 ggcaaatacc tgcataatct gttcgatatg agcatcggtc agaatgttgt tgttagtctc
     1021 ttttttgaac agttcgctgg catcaataaa ctgaactttg gtatccgttt tatgtttaga
     1081 cagcaccaga atattgacgg caatagtggt gccaaagaac agattcggtg ccagtgaaat
     1141 cacggtttcg acatagttat tgtcgaccag atactgacgg attttctgct ccgcgccgcc
     1201 acggtaaaaa atgcctggga agcagacaat cgcagcgcga cctttggccg aaagatagtt
     1261 cagcgcatgt aatacaaacg caaagtcagc tttggatttg ggggccagaa cgccagccgg
     1321 ggcaaaacgt tcatcgttaa tcagcgtcgg gtcatcgctg ccaatccatt tcaccgaata
     1381 cggcgggtta gaaacgatgg catcaaacgg tttttcatct ctgaagtgcg gctcagtcag
     1441 tgtattaccc agcttgatat caaacttgtc gtagttgatg ttgtgcaaaa acatgttcat
     1501 acgcgccagg ttataggtcg tatggttgat ttcctgacca aaaaaacctt cttcgatgat
     1561 atggtcatca aactgttttt tcgcctgcaa caacagcgaa ccggagcctg ctgccgggtc
     1621 gtagattttg ttaacgtggg tctgcccgtg catagccagt tgtgcaatca gcttagagac
     1681 gtgctgcggt gtaaagaact cgccgccgga cttaccggca ttcgccgcat agttagaaat
     1741 caggaactca taggcgtcac cgaacaggtc aatctgatgt tcatggaagt caccaagttt
     1801 tagcccttca acccctttca gaaccgcagc caagcgggca tttttatctt taacggtgtt
     1861 acccaggcgg ttactggtgg tatcgaaatc agcaaacaaa cctttgatgt cagcttctga
     1921 aggataaccg taagcagaac tttcgatagc aacgaagatg ctgtttaaat ctgcattcag
     1981 tctgtcatta gtatttgctt tcgcagctac gttgcagaaa agctgactgg ggtagatgaa
     2041 gtagccttta gttttgatgg catcgtcttt aatgtcatca gtaattacgc tgtcatccag
     2101 tttcgcataa cagatactgt catcaccggc ttcaatataa ctggaaaaat tttcgctgat
     2161 aaaacggtaa aaaagcgcgc ccaaaacgta ctgcttaaaa tcccatccat cgactgaacc
     2221 cctgacatcg ttagcaattt gccagatctg gcgatgaagc tctgcacgtt gttgaatact
     2281 tgtcattttc atccacttat ttcaggttta agtaattgga tgtgattcta cagcaacttg
     2341 gatgctttag cagttcggac attaggtaac gaatgaccta attagagctt tttggaaaag
     2401 cggtattgtc ggtgcgattt tagctatgtc tgggtgtgcg atggtgcaat acaatgacgg
     2461 tgaaaaggtt agcatccagt tcgatggctg gtattggctt ggatagcctg ccaaaaaacg
     2521 cagataaagc ctgtcagcaa tacggaaagt ctaaagctgt atatctgcat agcgcgaacg
     2581 ctaaccctca tcttgcgcct ggtacgggtg ttcagaatac catctggaaa tgtgagcctt
     2641 aaacatgagc aactggaaca tagcagccaa aagccaggaa gaaaggaata aagttaacgt
     2701 tgacctggct gccagggggg ttgcctacaa agagcgattg aacatacccg tcatagcgga
     2761 acaggtagcc cgtgagcaac ctgagaacct gcgcacctat ttcatggaac ggctacggca
     2821 ctaccggcag ttaagcctcc tgctgccaaa aggaaacgat ccggtatatc agaaagaaga
     2881 tatagccaaa aaataacgcg ccaccagacg agcgcaaagc atcgcgctat tgcacacttc
     2941 aagcatcttt aatgtatact gtatggataa acagtactct gaggtgaaaa cgctatgggc
     3001 ttcccttctc ctgcggcgga ttatgctgag agccgtattt ctcttgatca gcagataatt
     3061 agacatcctt cagcgaccta cttcatgagg gcagctgata gtcatcatcg tgagggaata
     3121 ttacagggtg cattgctggt ggtcgattcc tcacttaccc cggttgatgg ttctctgctt
     3181 gtatgcgctc tggatgggga atatcgcgta aaaagatacc ggaagtaccc acgtcagcat
     3241 ctggaggatt taagaaccgg taagaaggaa gcattgccaa aggatgacga tggatgcacg
     3301 ggcagcaatg ccgtgtttgg tgtgatcact cacattatca acgacgcaag aagtggcgag
     3361 tttgatgatt gtcccgtgat gtaggagaac tgattaggcg gtgcaatgca ccgcctttta
     3421 tcacactgcg cggaatgcga tttcgccagg tattacttca ccttgccaat acatttgggc
     3481 ggcaacgcga tctgcgaggt cacgataaat agccgtaaat tcgctatctg gacgactaat
     3541 aacggttggt gttccgttat ccagatcttc acgaagagag atatgaagtg gcatttggcc
     3601 taacaactgc gtgttgtatt tctcggccag tttctgtgcg ccaccggtgc caaaaattgg
     3661 ctcgtgatga ccgcagttac tgcaaatatg cacactcatg ttttcgacga tacccagtac
     3721 cggcacttcg actttttcga acatcacaat gcctttcttc gcatcgatca gcgcgatgtc
     3781 ttgcggcgta gttaccacaa ccgcaccagt tacaggaatg ttctgcgcca gcgtcaactg
     3841 aatatcacca gtgcccggcg gcatatcgag aacgagatag tccagatcag gccatagagt
     3901 ttcctgcaac atctgcatca gcgccttgct ggccatcggt ccacgccaca ccattgcatt
     3961 gtcgtcggtg accagataac caatagagtt ggttgccagg ccatgagaca tgataggtgc
     4021 catgtgagta ccgtcaggtg aggttggacg ttggttttcc gcgcccagca tggttggaat
     4081 tgatggacca tagatatcgg catccaaaat accaactttc gcaccttcag cagccaacgc
     4141 cagtgccagg tttaccgccg tggaggattt acccacgccg cccttgcctg agctgacggc
     4201 gataatgttc ttaacgccat taatgcctgg ttggtttttg acgcgcttaa gcgtggcaat
     4261 gttgtacgac agcttccagt caatagcctt tgcgccagtg atacggagca gatcaccact
     4321 acattgctct ttcaggtctt caaaaggctt attccacacg aaaggcatga ttagttcgac
     4381 atgcagtgtg tcatccatca acgcaacatg gtgtaacgct ttaagcgtag tcaggttgtg
     4441 tttcagggtt gggtgctgaa aattagccag cgtaccggct accattgctc tcagggcatc
     4501 cggcgatttg gactcgctca tcccgtctcc tttattttaa tttgcgcaat tgtcgccttg
     4561 tagtgtactc cagctacgac atttaatcat ttatgagaaa tgctgttatc acatggcaga
     4621 cataaggcca ttttgttact atcaagcccc ttttcactac aaagaagtaa tgcctactat
     4681 gacccaagtc gcgaagaaaa ttctggtgac gtgcgcgctg ccgtacgcta acggctcaat
     4741 ccacctcggc catatgctgg agcacatcca ggctgatgtc tgggttcgtt accagcgaat
     4801 gcgcggccac gaggttaatt tcatctgtgc cgacgatgcc cacggtacgc cgatcatgct
     4861 gaaagcacag cagcttggta tcacaccgga gcagatgatt ggcgaaatga gtcaggaaca
     4921 ccagactgat ttcgcaggct ttaacatcag ctatgacaac tatcactcga cgcacagcga
     4981 agagaaccgc cagctgtctg agcttatcta tactcgcctg aaagagaacg gttttattaa
     5041 aaaccgcact atctctcagt tgtacgaccc ggaaaaaggc atgttcctgc cggatcgttt
     5101 tgtgaaaggc acctgcccga aatgtaaagc gccagatcaa tacggcgata actgtgaagt
     5161 ctgcggcgca acatatagcc cgactgaatt gatcgagccg aaatcggtgg tttctggcgc
     5221 taccccggta atgcgtgatt ccgaacactt cttctttgat ctgccttctt tcagcgaaat
     5281 gttgcaggca tggacccgca gcggcgcgtt gcaggagcag gtggcgaaca aaatgcagga
     5341 atggtttgaa tccggcctgc aacagtggga tatctcccga gatgcgcctt acttcggttt
     5401 tgaaattccg aacgcgccgg gcaaatattt ctacgtctgg ctggacgcgc cgattggcta
     5461 catgggttcc ttcaagaatc tgtgcgacaa gcgcggcgat accaccagct tcgatgaata
     5521 ctggaagaaa gactccaccg ccgagctgta ccacttcatc ggtaaagata ttgtttactt
     5581 ccacagcctg ttctggcctg ccatgctgga aggcagcaac ttccgcaagc cgaccaacct
     5641 gtttgttcac ggctatgtga cggtgaacgg cgcgaagatg tccaagtctc gcggcacctt
     5701 tattaaagcc agcacctggc tgaatcattt tgacgccgac agcctgcgtt actactacac
     5761 tgcgaaactc tcttcgcgca ttgatgatat cgatctcaac ctggaggatt tcgttcagcg
     5821 cgtgaatgcc gatatcgtga acaaagtggt taacctggcc tcccgtaatg caggctttat
     5881 caacaagcgt tttgacggcg tgctggcaag cgaactggct gacccgcaac tgtacaaaac
     5941 ctttactgat gccgctgaag tgattggcga agcatgggaa agccgcgaat ttggtaaagc
     6001 tatccgtgaa atcatggcat tggctgacct ggctaaccgc tatgtcgatg aacaggctcc
     6061 gtgggtggtg gcgaagcagg aaggacgcga tgccgatctg caggcgattt gctctatggg
     6121 cattaacctg ttccgcgtgc tgatgacgta cctgaagccg gtactgccga aactgactga
     6181 acgtgcagaa gcattcctga atacagaact gacctgggat ggtatccagc aaccgctgct
     6241 gggccataaa gtgaatccgt tcaaggcact gtataaccgc atcgatatga agcaggttga
     6301 agcactggtg gaagcatcca aagaagaagt gaaagccaca gccgcaccgg taactggccc
     6361 actggctgac gacccgattc aggaaactat cacctttgac gacttcgcca aagttgacct
     6421 gcgcgtggcg ctgattgaaa acgcagagtt tgtagaaggt tctgacaaac tgcttcgcct
     6481 gacgctggat ctcggtggtg aaaagcgtaa tgtcttctca ggcatccgtt ccgcttatcc
     6541 agatccacag gcactgattg gtcgtcacac cattatggtg gctaacctgg caccacgtaa
     6601 aatgcgcttc ggtatctctg aaggcatggt gatggctgct ggccccggcg ggaaagatat
     6661 cttcctgtta agcccggatg ctggtgctaa acctggccac caggttaagt aattaaacaa
     6721 cctctcttcg caatgcccgg caacacgccg ggcattttca tatagacaca atcccttctg
     6781 atttacacag tacctaaaac aatttgtttt ctgccttttg ttgatggaga taataaacca
     6841 cataagaaaa caacttgttt tggagtggtt gaaatgttga acattaccca agcctaccag
     6901 gtggcattga tcagctcatc tcatacatca gaggaagatg ctcgccgatt catgatcgca
     6961 agcaatgagc tggattgggt ttcacaaatt gagtacggtt gcattgtcca cgccgggtta
     7021 caggacgatg actggaaaga tgagttgcgt cgatatggta tatccgaggg cgcgattgcg
     7081 aacattcaga aagtacttga tgtaggcttt gactctgtac attttgattg tggtgcaccg
     7141 gtcgttgaag ggctagagtg ctggcaatgg taatccggca atcgcttcat actagacaac
     7201 ggagaattta tgcttaaaga agactcatat ctactaaagg cgcttgaaat gcgtggcgtt
     7261 gaacctacag tgaaaaacgc tcacgatatc taccttcagt tttcaccgga gctgaaacgg
     7321 ttcataaatc aatacggggt taatgactca agtgttaaag caatgataga tgatgaatta
     7381 gaccatctca tctaatcgtt tcctatgata atggttttca ctttgagatt gttatttttc
     7441 atagctaaag ctgatgtttg tggaaggtgg attgataaac ggatgcgtat ttaggttata
     7501 caagtgagaa aattgataat atataaacgc gatttttatg tgatggaatg gatgtgatgg
     7561 aaacccagaa ccaacaaaca aaacctctga ataacattta tgcgtggatt ctagcgttca
     7621 tgcctttctt ctttggagta tcgccggaag agtataccaa ttacgctatt ataattgggg
     7681 gtgtaactac catcggtttg ttagtagctg acagaatggc tctatcggag tctggctgtg
     7741 aaccaccttc aattctatgg ggggggtatt cctcctgcct gtctatctct ggaagcgcgc
     7801 gactattaca aaaggcagca gagtgccatt tatcataatc gttgcgtcgc tggcttgggt
     7861 tatcttcatc ggcatcccac acaaggacga ggttgctctt gaagaagcag cctgccctct
     7921 cgtgacaact attctgaaag agaataacgg ttcgactgca ccaaaatgta tgaaggtgac
     7981 aatagaggac aaggtgacgg ataagttcta tagagcaacg gctacactag acaatggtaa
     8041 tgacattaac attactctgg aacttaccgg cgacaggaac ttctacgtcc gtgttccaaa
     8101 tgtttatctg aacaactaac ggttgcaact cattgataat ggcggtcaca atttgattct
     8161 tttctataag aatccgttga tcgccactac tccccttcca tacccgcaat cacatcctta
     8221 aatatcaata agttatagtt gtttatacaa tcaagaaaac aactgaataa cagatcaacg
     8281 aaacatcaac gctttcaccc gatttggtct aaaaaattga cgttttcaca catcactgct
     8341 tatacccgta attctacgca gcagacagcg ccacagcgtc caattttcac cacagacgac
     8401 ccaacacact accaacaaaa acacacgctc aaaatcgttc ccgttgcgtt acataaccat
     8461 ataaacaact tattttcagg tataacaaaa caactaaata acacatatgc cattaccaaa
     8521 aacaaccaac ctctttatgg caggctaccg aaaagaccca cctcttcttc ccaggctacc
     8581 ggacaaccaa cctaactttc acaggcaatc cgaaaacatt catacgcgcg atacccacga
     8641 cacgccatta cccaaacagc gaagaaacat cagaatcact gaaaccccaa cgaagcctgt
     8701 aaccgcaagg tttctctcaa tcccaaaaga cacgaaacat aacaatcacg ggaaacgcaa
     8761 ttacccaata cacgaataac tacaaaagcc tttcacacac tccagacaaa caactcaaac
     8821 acaataacga aaggaatcgc ccacctgcca ttactccata cacggagaag aacaatcacc
     8881 aaaacgacga gaacacccca tagaagaacc accgcaaaac taacgaaacc acatacaccg
     8941 acagagaaaa caacacaccc aattcaccaa cagaaatagt cgccgtatag aacgttctgg
     9001 aaggagtgag cattaacgcc tatagagagg ttgagacaga taaaacacga agaaaacaca
     9061 acatgtatag cgaacaagac aggtatgtat aagtggggag ggaaggaggg gtgtcgcctc
     9121 ctttttcgta tttattcaac tcctgatttt atcccccgta acacccctac ggtcagcctt
     9181 cggtccatac agggaaaagg ttgcatcccg ctacagaaac ggctgggttg ccttcgggga
     9241 acggctggag gttttcaggg aaacggtcga gttgcctgtg tggatttcgg gaaaaggctc
     9301 gatgccggtt caggaacggt ggcgtaccgg tatagcgtga gggagagttg cggctgtcca
     9361 cctgtaatgt gcgggaagaa gagaggcgct cacgtgcagg cgaacttact taccgttgga
     9421 ctccctctga atgcccagct acagcgtaat tgtgccagac agtcagcggg tcacaatcga
     9481 tttcagtagg ttttcgggga gaggattatt ctgcccacca gccaccttcg gccactaaat
     9541 cagaataatg taaaaagtgt actcggtact gaacctgaaa actcactgcc aaaaccacat
     9601 tcaccggaat aaaatccttt ttcgcctgtg cgttattgcg ataataacac catcaagaaa
     9661 acatgttgtt taaggattgc tttatgtttg caaatatcga catcaaccaa attaagaaat
     9721 tgactcaaaa agagtttgac cagttttatg agttagaagg ttggtcttcc actctgatta
     9781 attcaagatg ggtgctggaa ctgatgactc gtgatgacgc acctgctttg atgatcagcg
     9841 acctgggtga agatgctgac tttatggata tgagcgaatt ttgtgtggac acatacaacc
     9901 gcagccagaa gtactacttc acatgcgata gcgaaaatga cgtgatttct aaggtctatc
     9961 ttcacctcgt ccagcattgg gacgttcagg agtttcttga ggtattcgcg taatcccaac
    10021 caaagccagc attgctggct taatttctct atagccgcag ggaagaacct gcatatcgct
    10081 catgcgcctg atcaatatgc tttgtagccg ttttcttact ggtattattt acgccattga
    10141 gaaaacaagt tgtttacgga gttgtaatga aaaaatcatt ggttcttggt ctggacaaag
    10201 accagaagag aaaagagaag cctgcgctgg ttgctcaatt aactttgctg gacatcgttg
    10261 ccaatggaac ctctattcgt ctgttccgtg aaacagcggt gtctttcgat aaaaacacct
    10321 ttactcgtta tgtaatgaat gttcgtcgcc agcgtggaaa aggctgggtg gcatttcaaa
    10381 gaatgtggcc ggaacatcag ctcgaactgg ctttgatgga agttaaccgc gtggcccagc
    10441 aagaaattca gagagcatca gtgatggcaa tagcctgata atgtgcaagt ggcaattagt
    10501 cgaccgtacg acagccccgc catccttacg gggctttttt gtattgtaag tacatactta
    10561 ctatcataga atataaaaat aaccaaaaca ctacggagag tgaaatgacc gttaaacgcg
    10621 aaaaactgac agttgatgtt tactatgcct ctgaaaccgc cgaaggtaag aatgtggcaa
    10681 aaatcaccgt tgttacgtac aacaccgaaa ctggtgccga agtccaggcc agtacgatcg
    10741 tgcgtaaagg tgatgcctcc gacggcgagt acgcgactca ataccagtcc attctcgatg
    10801 caactgaccc gctgctgctg aaaatcgaga actacttccg ccaggttgat gaagaggtgt
    10861 ttgaaaccat gatgaatatg gttaacaccg tattcgcctc caacctgaat accaacacta
    10921 cctggattgg tcagtacggc ctgcgcatta cctctggcat tcctgccgac accttaatcc
    10981 ctgaaagcgt attcgcttaa tcctctttaa atggcgcgta aaccgcgcca ttttattaag
    11041 cccgataaca atttgttttc tgccttatct gatttgtgaa aatgatttca ctgaagcaac
    11101 ttaataagga aaccatcatg ggacttgata tctatatcga gacacagcct aaaaacgatc
    11161 tgaataacga ggcatccaga aagcaggttg cttacttccg taagttcaat gcgctcgttg
    11221 ggtggatgga gcgcaacgta ggtgaagtcg aaaattgtga gcttttagaa ttaacgatga
    11281 atgacatttg ttttctgaag gctcatttga tgcacataaa cgaaagtaat tgcgaagagt
    11341 acttgcctac ccgggaaggt tttttcttcg gcagtcagga gtacgatgaa ggttactggc
    11401 atgatgtggg ggagttgaaa gagcttgtgg aagatctgat taagaaccac gactttcaca
    11461 ataacagact gaccttctgc gcctggtggt aaatatgggc gatttcaaga aacgcctgaa
    11521 ggagagagcc gagatggtca gaaagcgaaa cgcctcttct gtcatcagat acgcaaggca
    11581 gtttagtcgc aacaacaaat cagttgagga aaagatcctt agcgtaatcg ggcgataatt
    11641 attaaggcca ccagcattgg tggtcttaaa tgacttttaa taaccgaagg ttagtttgaa
    11701 acctggctat caacttccag aacctttctt gtgtattcag cctgtattaa cttcttttat
    11761 gccttatcta cactgcgata attacaccaa caagaaaaca atatgtttac gcagtgagga
    11821 tatgctcatg actgatttca ccattacccc taaagcacaa aatgtattcc ttgaatcatg
    11881 gctggacttg ccggaaacag aacagcaaga aatggatcat gtggaatatg acgagcaggt
    11941 aagcacgcga ttcttccact ttgagggctg cgtttacgac attgccgact tcatgcgaga
    12001 tgaccgcttc cctgaatggc acgcaagcta tccactaaac gccttcgcca tgctgatgat
    12061 ccgtgtggat gattcagggg ataccatcga catcggtctg ctccactaaa gagtaaggcc
    12121 accagcattg gtggccttaa atgaccatcc tgtttcccgc aggctaaaaa caccaacctc
    12181 ttacctccag gcaaccgaca agcccacctg ttctcgtccg gctaccgcaa ctttccactt
    12241 tgacgcctta ttcgtacaac gataattaac accaacaaga aaacaatttg ttatttacga
    12301 taaggaatta atcatgaatt ttatcgctac tgtaaacgca cccgcacatg gcaatatcgc
    12361 tgtaacgttc tctgacattg aaaaacgagt acttggtgca tggcgcgaca atgagacggt
    12421 agaactgtca gcacaagaaa aatgcattat tgcacgcgac atcattggca atcgtcgtta
    12481 ctcgcgggta tttgagaaag cgtatgtggt aaattctggg ttcggaacgt tcgtctttcc
    12541 ggtgcgctca gggcgattct gccagtccaa gctgattgag ttcgctacgc agatttctgt
    12601 ctggattaaa actcaatcgt cgttcaaatt ttccgacgat gaagcagtat cgcaggggat
    12661 gcggatcgcc aacaatgcga ttaaatgcaa aaacattacg tataccgctg gcgttgacac
    12721 atggaaactg ttttgcgcta actttatgct gaatgtatac gcaagcaacc gcatccacat
    12781 ccttgatggc gtgtaactga gaagagggcc agaaacggcc ctttctctat agccaccagc
    12841 tgccgcaggg aaattttcag aaacggcgag gaacgtattc atgagccgac gggaaacggc
    12901 caggattttt tcgggaaacg gctgcattcg cctttatgta gaaaaaaaca tcgggaagct
    12961 ggtggaatcc aaccagcggt tgtcggacag gcgagcgggg aaaattatga tgactttcgt
    13021 cacctgagac atccagattt ctttcgtagc ataatcacat acgtgattaa gtggtgtgat
    13081 tatgtgaaaa atcacgcgca cataatacgc gagcggatac ggaacaaaac aaaatgccga
    13141 tccgcgccga caaataaacg cggatcataa agcaagacta aaagccaatg attaactatg
    13201 cgctataacg cgttttaagc gcgttaatgt gttaagtaat gggtatgtac tggcaaggat
    13261 ataaaagcgc gtctatggcg ttattttggc gcttattttt atgttgttgg agtgagttaa
    13321 agacaataaa aaacgcgcca ttgatggcgc gttattgtgg aagtattgga aacgaaaaaa
    13381 gcgcccatag tgggcgctcg attttatttg tgtaaactga ttttaaatcc catttctacg
    13441 aacgctttta acattaaaaa tagatcagcg tcgttcacgt ctgctttttt tcgctcctgg
    13501 tcgctcaata agtcaatttt gcgcgtcgtt tcatctataa gctcgactac gcgcccagcg
    13561 taaccagcga tcccagcgat gcgattaaca taatattcat tatggacgtt aacgccagcg
    13621 ataagaacaa acatgattaa gctccttaaa aaaaattaaa tagaattttg aagttttccg
    13681 attaatgcgc ttaagcgtaa gtattcacgt ttgcgcttgc aatctcgctt gctgttaagc
    13741 aaatcagaaa acgtaaaatt taattggtcg cgttgcgcga tcaaatcgtc gattaattcc
    13801 agtttataag cgcgataatt tgcgcgatat tctgcgcgga tcttgtcata gctgaccatc
    13861 tttaaagctc ctttaaagcg cccattgtgg gcgcttgatt ccattaatta cgctttaaaa
    13921 gcatcagcta aataattgta gaaatcattc ttgataaagc gatattgctg tgtaccagct
    13981 ttggcgctcc cagctccttt gactttttca acaagtccga gacgttcgca aagattgatc
    14041 aactggttgg cttgagtata gccagcgtcc aatttaattt cacacgcttt tttagcttca
    14101 ttcattaaat cgaaaacagc gccattggtg aacgtgtcga tctcgtcgtt aatcatatcg
    14161 attaaagcga atacacgaga tccagacata tcagcgatgg aataaacaca ctttccagct
    14221 ttaatggatt taaccagata aaccagtttt tccagagaat aactattagt cattgcatca
    14281 cggaaaaata cttctggcgc ttgtttgctt gctttgatcg catagtagaa aacagagcat
    14341 aatttttcgt cttcaacagc gtttacaacg ttgttgatga agtaagcaag tttagtggtt
    14401 gcagcttgca tatttgcttt gtctgcttta gtgtgcgtac catttttata atgttcgtta
    14461 tatgtttgag ttgccagatc tgctttttcg cgcagctcgt cggatacaat ggcagcagct
    14521 tcaatgatgg attttttaga aatgataatg ttagccatga ttttattcct tagtataaat
    14581 attaaaattt aactcaa
//
LOCUS       DAZGVQ010000123        14510 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000123 DAZGVQ010000000
VERSION     DAZGVQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.124"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            57..623
                     /locus_tag="ACHLAG_003737"
     CDS             57..623
                     /locus_tag="ACHLAG_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0656883.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            674..3133
                     /locus_tag="ACHLAG_003738"
     CDS             674..3133
                     /locus_tag="ACHLAG_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0656884.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGILSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3148..3879
                     /locus_tag="ACHLAG_003739"
     CDS             3148..3879
                     /locus_tag="ACHLAG_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0656885.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3876..4937
                     /locus_tag="ACHLAG_003740"
     CDS             3876..4937
                     /locus_tag="ACHLAG_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0656886.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5089..6135
                     /locus_tag="ACHLAG_003741"
     CDS             5089..6135
                     /locus_tag="ACHLAG_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP0656887.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRFPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6132..6923)
                     /gene="nei"
                     /locus_tag="ACHLAG_003742"
     CDS             complement(6132..6923)
                     /gene="nei"
                     /locus_tag="ACHLAG_003742"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFP0656888.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6959..7693)
                     /gene="pxpA"
                     /locus_tag="ACHLAG_003743"
     CDS             complement(6959..7693)
                     /gene="pxpA"
                     /locus_tag="ACHLAG_003743"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFP0656889.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQLGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7683..8615)
                     /gene="pxpC"
                     /locus_tag="ACHLAG_003744"
     CDS             complement(7683..8615)
                     /gene="pxpC"
                     /locus_tag="ACHLAG_003744"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFP0656890.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8609..9265)
                     /gene="pxpB"
                     /locus_tag="ACHLAG_003745"
     CDS             complement(8609..9265)
                     /gene="pxpB"
                     /locus_tag="ACHLAG_003745"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFP0656891.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9288..10031)
                     /gene="ybgI"
                     /locus_tag="ACHLAG_003746"
     CDS             complement(9288..10031)
                     /gene="ybgI"
                     /locus_tag="ACHLAG_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFP0656892.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVLRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10302..11783
                     /gene="dtpD"
                     /locus_tag="ACHLAG_003747"
     CDS             10302..11783
                     /gene="dtpD"
                     /locus_tag="ACHLAG_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFP0656893.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMPGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSSMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11825..13243)
                     /gene="phrB"
                     /locus_tag="ACHLAG_003748"
     CDS             complement(11825..13243)
                     /gene="phrB"
                     /locus_tag="ACHLAG_003748"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFP0656894.1"
                     /translation="MITHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13240..13749)
                     /locus_tag="ACHLAG_003749"
     CDS             complement(13240..13749)
                     /locus_tag="ACHLAG_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFP0656895.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     LRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13900..14510)
                     /locus_tag="ACHLAG_003750"
                     /pseudo
     CDS             complement(13900..14510)
                     /locus_tag="ACHLAG_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3509 a         3871 c         3509 g         3621 t
ORIGIN      
        1 aaataagata ttcttgcacc gatatacttt tttcttttct gttaaaggac tccgttatgt
       61 ttaaaggaca aaaaacattg gccgcactgg ccgtatctct gctgttcaca gcacctgttt
      121 atgcggctga tgaaggatcc ggtgaaattc actttaaagg tgaagttatt gaagcaccgt
      181 gtgaaataca tcaggatgat attgataaag aggttgaact cggtcaggtg accaccagcc
      241 acattaatca gtcacatcac agcgatgccg ttgctgtcga cctgcgctta gtcaactgtg
      301 atctggaaaa ctccagcaac ggttccggtg gcaagatttc taaagttgca gtcacctttg
      361 atagctcagc gaaaaccacc ggcgcagatc cgattctcaa caacaccagc acgggtgaag
      421 ccaccggcgt cggcgtacgt ttaatgaata aagatcaaag caatatcgtt ttaggcaccg
      481 ctactccaga tatcgacctg gctccgacct ccagcgaaca aacgctgaat ttctttgcct
      541 ggatggaaca aattgatcag gctacacctg taacgccagg cgcagttacg gcgaatgcaa
      601 cctacgtgct ggattataaa taagtattac tttggcggcg ctatcgccgc ctttcataaa
      661 atatttatca ggtatggaca ccgtgaatat ttatcgactc tcttttgtat cctgcctggt
      721 cgtggcgatg ccttgcgcat tggcggtcga attcaacctt aatgttctcg ataaatcaat
      781 gcgcgaccgc attgatattt cattattaaa ggaaaaagga gtcattgctc ccggtgagta
      841 ttttgttagc gttgcggtaa ataataacca aatcagtaac gggcaaaaga ttaactggca
      901 caaaaatgac gataaaacca ttccgtgcat caatgattta ctggtcgata aatttggctt
      961 aaaacctgaa gtccgtcagt cgttaccatt gataaatcag tgcgtcgatt ttagctcccg
     1021 acctgaaatg ctcttcaatt tcgatcaagc caatcagcaa ctaaatatca ccattccgca
     1081 agcctggctg gcgtggcact cagaaaactg gaccccaccc tccacatgga aagaaggtgt
     1141 cgccggtgtc ctgatggatt acaacttgtt tgccagcagc taccgcccac aggatggcag
     1201 cagcagcact aatcttaacg cctatggaac agccggaatc aacacggggg catggcgctt
     1261 acgcagtgat tatcagttga atcagactga tagcgatgat aaccatgaac agtcaggcga
     1321 aatatcacgt acctatcttt ttcgcccgtt accgcaatta ggttcaaaat taaccctcgg
     1381 cgaaacggat tttagttcca atattttcga cggtttttct tataccggcg cggcactggc
     1441 aagtgacgat cgaatgttgc catgggaact acgcggctac gccccacaaa ttagcggtat
     1501 tgcacagacc aatgccacgg tgacgatcag tcaatcaggc cgcgtcattt accagaaaaa
     1561 agtcccacca ggcccattta tcattgacga ccttaatcag tctgttcagg gcacactgga
     1621 tgtcaaagtg acggaagaag atggtcgggt gaacaatttc caggtttcgg cagcatcgac
     1681 gcccttcctg actcgtcagg gacaggttcg ctataaactg gccgcgggtc agccacggcc
     1741 ctccatgtca catcaaactg aaaatgaaac cttttttagc aatgaagttt cctgggggat
     1801 actgtcaaac acctcgctgt acggcggcct gctgctttct ggtgatgact accattctgc
     1861 cgcaataggt atcgggcaaa atatgctgtg gcttggcgcg ctgtcgtttg atgtcacgtg
     1921 ggccagtagt cactttgata ctcagcagga cgagcggggc ttaagctacc gttttaatta
     1981 cagcaaacaa gtggatgcca ctaacagcac gatttccctc gccgcttatc gtttctccga
     2041 tcgtcatttt cacagctacg ccaactatct ggatcacaaa tacaacgaca gcgatgcgca
     2101 ggacgaaaaa cagacgatca gcttatctgt gggccaaccg attaccccac taaacctcaa
     2161 tctttacgcc aacctgctac atcaaacctg gtggaatgca gacgcctcca cgaccgccaa
     2221 catcacggca ggttttaatg ttgatattgg tgactggaga gatatctcta tttctacgtc
     2281 attcaataca acccattacg aagataaaga tcgcgacaac cagatttacc tgtcgatttc
     2341 gctccccttc ggtaacggtg gtcgggttgg ctatgacatg caaaacagta gccacagcac
     2401 cacacaccgc atgtcgtgga acgatacgct ggatgaacgt aatagctggg gcatgtctgc
     2461 cggactgcaa tccgaccgtc ctgacaatgg agcccaggtg agcggtaact atcagcacct
     2521 gagttcagcg ggtgagtggg atatttctgg tacctatgcc gccaatgatt acagttccgt
     2581 cagcagcagc tggagcggtt ctttcaccgc aacccaatat ggtgcagcgt ttcatcgccg
     2641 cagctccacc aatgaaccac gcctgatggt cagcaccgat ggcgtggcag atattccggt
     2701 tcagggcaat ctcgactaca ccaaccattt tggcattgcg gtggtgccgt tgatttccag
     2761 ttaccagcct tccaccgtgg cggtgaacat gaatgactta cccgacggcg taacagttgc
     2821 agaaaacgtt atcaaagaaa cgtggattga aggcgcgata ggttacaaat cactggcttc
     2881 ccgttccggt aaagacgtta acgtcatcat tcgcaacgcc agcggtcagt ttcctcccct
     2941 cggagcggat atccgccagg atgacagcgg cattagcgtg gggatggttg gcgaggaagg
     3001 acatgcctgg ttaagcggag tcgctgaaaa tcaaaagttt accgtggtct ggggtgatag
     3061 ccagcattgc tcgctccatc ttcctgaaca tatggaagac accgcaaatc gcctgatttt
     3121 accttgtcat taattaagga aatgaaaatg acatttatga aaggactacc ccttttgttg
     3181 ttggttgcca gtctgtgcag ccacgctgca ctacaacccg atcgcactcg tattgtgttt
     3241 aacgctaatg ataaagctac cagtctgcgg gtggataatc gtagcgataa actcccctac
     3301 ctcgcttatt cctggctcga aaatgaaaaa ggggagaaaa gcgatgatct tctggtcgcc
     3361 ctaccgccga tacaacgtct ggaaccgaaa gcaacaacgc aggtacggat agtaaagcaa
     3421 gcctcgacca caaaattacc gggcgatcgc gaaacgctat ttttctacaa tatgcgtgaa
     3481 attcctcccg ccccggaaaa aaacagcgac catgccgttc tgcaagtcgc cattcagagt
     3541 cgtatcaagg tgttctggcg accggctgca ttacgcaaga aagcaggaga aaaggtcgaa
     3601 ctgcagttac aggtcagcca gcagggcaac caactgacac tgaaaaatcc taccgcgtat
     3661 tatctgacca ttgcctacct gggacgaaat gaaaaaggcg ttctccccgg cttcaaaacc
     3721 gtgatggttg cgccttttag tacggtcaac acaaacaccg gaaattatag cggcagccag
     3781 ttttatctcg gttatatgga tgattacggc gcgttacgca tgacgacgct caactgcagc
     3841 ggacaatgcc atttacaagc cgtggaggcg aagaaatgag tgctggcaag ggagtgttgc
     3901 tcgtcatctg tctgttattt ctgccgttga agtctgcgct tgccctgaac tgctactttg
     3961 gttcatccgg tggctcggta gaaaaatcag aagcgattca accttttgct gtaccaggca
     4021 atgccaaact cggcgataag atttgggaat ctgacgatat taaaattccc gtctattgtg
     4081 acaacaatac caacggcaat tttgaaagtg agcatgttta tgcctgggta aacccctacc
     4141 cgggggtgca ggatcgctat tatcaactcg gcgtaactta caatggcgtt gattatgacg
     4201 ccaaccaggg aaaaagccgc atcgacacta accagtgtat tgacagtaag aacattgaca
     4261 tttatacccc tgagcagatt atcgcgatgg gatggcagaa caaaatttgc tcgggtgatc
     4321 ccgctaatat tcacatgtcg cgtacctttc tcgctcgcat gcggttatac gtcaaaatac
     4381 gagaaatgcc gccgcatgat tatcaaagta cgcttagcga ctatatcgtc gtgcaatttg
     4441 acggtgccgg tagcgttaac gaagacccta ctgcccaaaa cctgaaatat catattactg
     4501 gtctggaaaa cattcgcgtg ctggattgca gcgtcaattt ttccatttca ccggaaacac
     4561 aggtgattga ttttggtaaa tttaatttgc tggatattcg tcgccacaca atgtcgaaaa
     4621 cattcagtat taagacgaca aaatcacaaa atgaccaatg caccgacgga tttaaagtca
     4681 gttcctcctt ttataccgaa gaaacgttgg ttgaagaaga taaagcattg ttgattggta
     4741 atggtctgaa gctacgtttg ctggatgaaa acgcctcgcc ttataccttc aataaatatg
     4801 ctgaatacgc cgatttcacc agcgacatgt tggtctacga aaaaacctat acagctgaac
     4861 tttcgtccat tgcaggtacc cccatcgagg ctgggccctt cgatacagtg gtgcttttta
     4921 aaattaacta taactgattt gccatgatgc cctttgcagc cggaaagggc atctctttca
     4981 gaaaattgct gattttcccc ttttccttga ttgctgttat tttttcctgg cccggctttc
     5041 tttatgctgt atcgatacac caaagagaaa ataatgaggg agcgaaggat gccagttttg
     5101 cagtggggaa tgttatgtgt gctatcactt ctcctttcta ttggtttcct cgcggtacat
     5161 ctcccggcgg cgctattact tgggccgatg atcgccggga tcatcttcag tatgcgcgga
     5221 attactctgc aacttccccg ctccgctttt ctcgccgcgc aggccattct tggctgcatg
     5281 attgcgcaaa acctcactgg ttcgattctc accacgctgg ccgccaactg gccgatcgtg
     5341 cttgctattt tactggtgac gctgctttcc agcgccatcg tgggttggtt attggtgcgc
     5401 tatagctcat tgcctggaaa taccggtgcc tggggttctt ctcctggcgg cgcggcggca
     5461 atggtcgcta tggcgcaaga ttacggcgca gatattcgcc tggttgcatt tatgcaatat
     5521 ctgcgagtgc tgttcgtcgc gggggccgct gttttggtca cccgcatgat gctgggtgat
     5581 aacgctgaag cggttaacca gcaaatcgtc tggttcccgc cagtgagcat taatctcctg
     5641 cttacgattt tgctcgcggt cgttgccggt acggcgggat gcttgctacg ttttccttcc
     5701 ggcacgatgc tcatcccgat gctggcgggc gcagtgctcc agtctggtca gctcatcacc
     5761 atcgaactcc cggaatggct gcttgcgatg gcgtatatgg caattggctg gcggattggt
     5821 cttggtttcg ataaacaaat cttactgcgg gcactacgcc cgctaccgca aatcctgctg
     5881 tcgatttttg ctctgctggc tatttgtgcg ggtatggcgt gggggctgac ccggtttatg
     5941 cacattgatt ttatgactgc ctacctcgcc accagtcccg gcgggcttga taccgtggcg
     6001 gtcatcgccg cagggagcaa tgccgatatg gcgcttatta tggcgatgca aaccctgcgc
     6061 ctgttcagta ttttgctgac ggggccagcc attgcgcggt ttatttcaac ctatgcgccg
     6121 aagcggtcgg cctagtgctg gcagcctgga caccagtaaa acgggcgcga ggacaacgtg
     6181 gttttctcaa tgatgccgcc acagcgttcg cacagttcgc catcacgatg aaaaacctta
     6241 aagcgaaaca gcgccccatg atgcttattc tcatccacct gcccccgcgt agcgtaagaa
     6301 aaccgaggaa tatccagtaa cgcgtgtgcg agtgcatcca gttgcgccgc attgaggtct
     6361 ttcgctttat gatttccagt caacccaacc tgccagagga tctccacccg caaataattg
     6421 ccaagtcctg ccagaaacgc ctgatcgagc agtaatccag caaactgacg gttacgaaag
     6481 cgcggtgaca ataaccgttc ctttaccacc tccggcgtca gattcggatc cagcacatcg
     6541 ggaccaacgc gttgtaaaaa cggatgcgtg gtcagttgtt ccggcctcaa catctcaata
     6601 tccgaggcgc tataaagcag aatggttttg tcagccgttt gcagttttac ccgcaatact
     6661 cgcgtggtct gcggctcttc gccggtatca accacgcgcc agacgccgta aagctgatta
     6721 tggctgtaga gcgttaagtc gttggaaaaa tgagttaaca acgccttacc acgcgtttcc
     6781 acatgggtaa cgtgttgacc gataagttgt gattgataag tttttaactg cgggaaggca
     6841 aaccagacat cagttagtgg tttgcctttg atcgccgcct ccaggttatc cgctgcacgg
     6901 cggatctccg ggccttcagg catggtgata tccttttttc attctttatt aatcggggtt
     6961 atgctgcgac aacaatctcc ttttcggcaa atgtagagcg tagtcggcgg gcgaacgcca
     7021 gtgcgtgctc gccgtcgcca tgcaggcaga ctgtttgcgc cgtgaccgtt gcccattcgc
     7081 cggtgatact tttgactctg ccgtgttgca ccatctctag cgtttgcgcc agcgcctgtt
     7141 cttcgttttc aatcaacgcg cctagctgac ttcgcggcac cagcgagccg tcagcctgat
     7201 aaccgcgatc ggcaaacact tcctcgcgcg ttgtcaggcc atattgcttg cctgcacgaa
     7261 tcagctcgct tcctgccagc ccaacgagaa ccaatgctgg atcgcaagcg tataccgctc
     7321 tggcgatggc gtctgccagt tgtgcttctt tcgccgcctg gttgtacaac atgccgtgcg
     7381 gtttgacatg acgcattacg ccgccttgcg cacgggcaat cgttgccagc gcgccaattt
     7441 gatacagcgt ctgggcgtaa acggtttctg gcggcagctg catggcgctg cgaccaaaat
     7501 tttccctgtc gggaaaactc gggtgagcgc caatcgcgac accatttttt attgcttcac
     7561 gcacgcaagc ctgcatggtt tgggcatcgc ctgcatgaaa tccacaggca atattggcag
     7621 aggaaaccag cgttaatagc tctgcgtcgc tggcgcagcc ttcgcccaga tcggcgttca
     7681 ggtcaatttt cattgtgtag ccgccacgct aattgttcga aataacgttg ttgatcttgc
     7741 cgcgctttca gtgcctcttc cagtgaacac tggacaaaat gaatcggctg accgagcgga
     7801 atttgcgcca gatggtacat atcagcctca atgatacagg caatacgcgg gtaaccaccg
     7861 gtggtctgtg cgtcgttcat caacacaata ggctgcccgt tatgcggcac ctgcaccaca
     7921 cccggtaaca aaccgtgaga taacagttcg cgatcggtgg tgcgttttaa aatttgcccc
     7981 tgtaagcgat agcccatgcg gttactttgc gggctaagct gccagggcga acgccagaat
     8041 gcatcctgcg aggcgcgatc gaactcatga tattccggcc ccggcaaggc gcgaatgcgg
     8101 ttgccccaca gcagctgttt aacgccctgc gcttccataa aatcacgctt cgatttgcca
     8161 atcgggagtc ggtcaccatc cttcagtaaa cggccttcca gcccgccaat ccccacgttg
     8221 agatcggtgc tgcatgaccc cattaccggc ggaacatcaa taccacccgc gaccgccaga
     8281 taactgcgca tcccgtgctg cgggcgttta agcgttaaac gctggcctgc tttcatcggc
     8341 aatcgccagc cggtccagac ggcattatca tccagccgcg cttcgcaacc ggcccctgtc
     8401 agagcaaacc acccatcagt ttcgaactca accgttaact gaccgagcgt gatctccagc
     8461 gcgggggcat tggcgtcatt acccaccagc aggttagcaa tgcgcagcgc aggcatatcc
     8521 agtgcgccgc agtggctgat acccgactgg cgaaaaccgt gacgaccgcc atcctgcaca
     8581 gtggtataca tgcccgcacg aataatcttc agcatactcc ctccttctgc ggtacaaagc
     8641 gcacgctgtc tcccggacgt aataagatgg gttcgtcacg cgccggatca aacaggctga
     8701 gtgaggtatg accaatcaac tgccagccac ccggcgttgc cagcggataa acaccagtct
     8761 gcggcccgcc gatcccgaca gaacctgccg gaacgagcaa gcgcggttca gcgcgccgtg
     8821 gcgtgtgtag ttgttccggc aaactcccga gatacgggaa gcccggttga aaacctaaaa
     8881 accagaccac gtattccacg gaggagtgca attcaacaac ctgtttttcg ctcaacccgc
     8941 aatgcgccgc gaccaccgcc aaatccggtc cgcctgcacc accgtaaacc accggaattt
     9001 caataaagcg agactcaggt tccagggcct cgctctcctc ccaccagcgt tgcaaacgct
     9061 ctatggcatc cagcgccagc gactcaggat tacgcaaaat caccgtgata ttgttcatgc
     9121 cgggaatggc ttcaaccaca ttcggcatat ccaccagacg ctgcgccagt cgccagatcc
     9181 gtttctggct agccagcgtc accggcggtt ccagttccag cactaccgcc gtttcaccta
     9241 tcagataaca acgcgctcgt tgcactttcg atccctctga ttattcgtta tgcaggatta
     9301 ggaatatcaa taaaggtcac atcaagatcg gtattttcat tcagccactc gctcaatgcg
     9361 cgaataccac cacgttcagt ggcatggtga cctgcagcat aaaaatgcaa tccctgctcg
     9421 cgggctgaat gaatggtctg ttcagaaact tcgccagtaa taaaagcatc cacgccaaaa
     9481 cgcgcggcgc tatcgataaa actttgcccg ccgcccgtgc accaggcgac gcgcaggacc
     9541 acctcaggtc cggtatcgcc acaccataac ggcttacgtc ccagacgcgc ttcaatccag
     9601 gaggctagtt ccagtcccgg cacaggcatg gtcagctctc cccacggcac caacggctca
     9661 atttcgccca tgaccgtgat ccccagtaac gccgccagct gtgcgttatt gcccagctca
     9721 ggatgtgcgt caagcggcaa atgccagcca tacaggttga tatcattcgc cagcaacgtt
     9781 tttaaacggt tacgcttcat tccgcgaatg accggagact ctcctttcca gaagtagcca
     9841 tgatgcacaa tgaccgcatc cgcgcccaga cgcactgcct catcgagcaa agcctggctg
     9901 gcggtgacac cggtaacaat tttttgcacc gtctctttgc cttccacctg caaaccattc
     9961 ggcgcgtaat cactaatcgc cgcgctgttc agtttttcgt taatcagttg ttccagttcg
    10021 gtgtttttca tcttcgcgcc ttccgtattc tgatagctac accgttaaca tagcgttacc
    10081 tcgccagttc gtcgatagcc aatcttttga catattaaga aacaaacccg acgccaaaag
    10141 acgcaaattg tttatatcat cacgcttcag ctaatgtata tttatgcaat cagacagcat
    10201 attgcataaa taatcagatc tgtatcaaag ttttaacttt aaggaaacac aagcacgccg
    10261 cgcaactatt acgtgacgtt gccgtcaatt gtggaattat tatgaataaa cacgcatcac
    10321 agccgcgcgc tatttactat gtcgttgcgc tgcaaatctg ggaatatttt agcttttacg
    10381 gcatgcgtgc cctgctgatt ctctatctca ccaatcaact aaaatacaac gatactcacg
    10441 cctacgagtt atttagcgcc tactgttcgc tggtgtatgt cacgccaatc ctcggtggct
    10501 ttttggcgga taaagttctc ggcaatcgca tggcggtgat gccaggggcg ttattgatgg
    10561 cgatcggtca tgtggtgttg ggtgccagtg agatccatcc gtcattcctc tatctgtccc
    10621 tggcgataat cgtttgtggc tatggtctgt ttaaatccaa tgtcagttgc ctgctcggcg
    10681 agctgtatga gccaaccgat ccgcgtcgtg atggcggttt ctcgctgatg tatgcagcgg
    10741 gtaacgtggg gtctattatc gcacctattg cctgtggcta cgcccaggaa gagtacagct
    10801 gggcgatggg ctttggcctg gcggcggtgg gtatgatcgc gggtctggtc attttcttat
    10861 gtggcaatcg tcatttcaca catacccgcg gcgttaataa aaaagtactg cgtgcgacaa
    10921 actttctcct gcctaactgg ggatggctgc tggttctgct ggtggcaacg cctgcgctga
    10981 ttaccgtatt gttctggaaa gagtggtcgg tatacgcctt aattgtcgcg actattattg
    11041 gcctgggtgt actggcaaaa atttatcgca aagcagaaaa ccagaaacag cgtaaggagc
    11101 tggggctgat tgtgacgctc accttcttca gtatgttgtt ctgggccttc gcacaacagg
    11161 gcggcagctc gattagcctt tatatcgacc gcttcgttaa ccgcgatatg tttggttata
    11221 ccgttccgac tgctatgttc cagtcgatta atgccttcgc agttatgctg tgcggtgtgt
    11281 tcctggcgtg ggtggttaaa gaaagcgtcg caggtaatcg taccgtgcgc atctggggaa
    11341 aatttgctct tggtctcggc ctgatgagcg ccgggttctg cattctgacc ttaagcgccc
    11401 gctggtcctc aatgtatggt cactcttctc tgccactgat ggtattaggc ctggcggtga
    11461 tgggctttgc ggaactgttt atcgacccgg ttgccatgtc gcaaattacg cgcattgaaa
    11521 tccccggtgt gaccggcgta ttaaccggca tttatatgct gctttccggc gcgattgcga
    11581 actatctcgc gggcgtgatt gccgatcaga catcgcaggc ttcgtttgat gcttccgggg
    11641 cgatcaacta ctccatcaat gcatatattg aagtatttga tcaaattacc tggggcgcac
    11701 tggcgtgtgt aggactggta ctgatgattt ggctgtatca ggcgctgaaa ttcagaaacc
    11761 gcgcgctggc gctggaatct taataggcca gcaccatacc tgataaaacg ctcgctgcgt
    11821 tttatcaggc tcctttcctc gcttcttcgt acgctgccag cgttcgcagc cttgcctctt
    11881 tgtgatcaac tatcggttgc ggataatcca gcatcacacc tgctttctgc gcccacttcc
    11941 acggctcatg caccgctttc cctggtacat cgcgcagctc cggtaaccat cggcggataa
    12001 actcgccctc acggtcaaat ttctcgccct gagttatcgg attgaaaata cgaaaatacg
    12061 gcgcagcatc agtacccgta gaagccgccc actgccagcc accgttatta gctgccaaat
    12121 caccatcaat cagctgcgac atgaaatatc gctcgccttc gcgccagtcg atcaacaaat
    12181 ctttcaccag aaaactggct gtaatcatcc gtagccggtt atgcatccag ccagtactgt
    12241 taagctgacg catagcggca tcaacaatcg ggtatcccgt tttgcctttc tgccaggcct
    12301 gtaaatgtgc gggattgctt tgccactgca cacgatccgt ccaggcaata aacggacaat
    12361 gtttacacaa cgaggggtaa tacgtcatca gatgacggta gaactcgcgc cagatcagct
    12421 cactaagcca gacactaccg gccccaccgt ccagcgcctg cggctgttcc gccaacaagc
    12481 gatgcaaaca ctggcgaggc gataacccgc ccgttgccag gctggcggac aaacgactgg
    12541 tgccttccac tgccggaaaa tctcgttgtt gctcatattc tccggcaccg ttctggcaaa
    12601 actggcgtaa ttgcgcaatc gccgcttttt cttccaccgg gaaatgcgca gtatcgaaag
    12661 actgacgagg ataattcagc gtaatggatg gcgcgggctc tatcgatccg ctactacgaa
    12721 cttttggcgc agcgacacac tccggcatcc cttcccgcag ccgtttcagc caggcattct
    12781 taaaaggcgt aaagactttg tacatctcgt gattaccggt catcaccgcg ccaggcggca
    12841 ggatcacgct gtcatcaaat ccttcacaca ccacgttacg cagcgctctt tcaacttcca
    12901 catcccgcgc ccgctcattc acttcatact gatagttata aaacaggtgg gtaacgctgt
    12961 tttccgcgca cacctgttta actatttcga cactggcgac aaagtcatcc acttcacgga
    13021 acaataaagg aatacctttt tccgcaagcg ctatttgtag cccattcagt tgagcattga
    13081 taagttcagc ctgacgcggc gacatgttat gcgtcgccca ctggcgtggt gtagcgatat
    13141 ataacgccag cacgcgtgca gacgaattgc ggcaggcggc agccagtgcg agattatcgt
    13201 gcagacgtaa atcctggcga aaccagacca gatgggtaat cataaaactc ctgaatggcg
    13261 caagttaatc acacgcggcc aaagttcgaa ataacgctgc cctgaaagcc aggcgtcagg
    13321 ataaagcgcc atatagtgtt tgatacgcat cagcggcgca agaagtggtt gctcgccacg
    13381 gcgataactg tcgataagcg ccgccaactg ctggcgttct gtggaatcaa tatgcgggcg
    13441 caaataaccc tgaacgtgca tcaaaacgtt ggtgtgatcg cgcgggttgg cgggatgaga
    13501 aagcaaaaca attacgcgtt ggtagtactg gttgtaaaaa tcatccagat tttgccactg
    13561 gtgaatatca gccacgaacg ggccaagttt gcggtactcc ggctgagaat gcgccagaaa
    13621 gacgagttta tagcggctgt gataatcgag aagcgccccg cgcgttaagc catgttcttt
    13681 cagtcgatgt aactcatgaa gggcgaagat acggattagg gtgctgtcat caaatcgttg
    13741 ctgattcatt actgctcctg aacggtttac aggagtaagt gtagacgttg agaaatgata
    13801 tttgtgaagt taatcctgtt gtagcaggac aaatttacga aagcctgctc cccgcaagga
    13861 ctgacgtcag gtagtttcta accatagctg ccctttctat tcaaacgtga aatcaataag
    13921 ctcatcaggg acatcgcaaa caatatgaag acgggtttct tctctgccgt gactcttttc
    13981 actaattgcg taactgtcat gcttgggatt atttaattct ttcagcggaa atttttcctc
    14041 gaaggcttta tttagccgcc cctggtttcc ttttacagcg aataaataat cacctccctg
    14101 tttttgtatc ttctctgcaa tatctttctg gcaacccatc gcatcggttg tgatgatttt
    14161 tcctttaata tccagcatgt taagaagttc agggatagct gtgatctcat tggatttctt
    14221 atccgtcttg atctgcccga tgaccagact gtgcattgtt gagaacgcac taatgacatg
    14281 aatcgctccc ctgcggcgac tcttgtcata agagtgccgg agcgtttttc catcaattgc
    14341 aatgacgtct ttatcatttg atgaatggca atcacgcatc cagttaataa agcactcgtg
    14401 aaattttgca ggactgatac aggatacaac tctggcaata gtatcgtgaa caggaatacc
    14461 attttcaaaa tcaccatatt gcttcaagaa atcgagatgt gtttccccga
//
LOCUS       DAZGVQ010000124        14354 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000124 DAZGVQ010000000
VERSION     DAZGVQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.125"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            26..382
                     /locus_tag="ACHLAG_003751"
     CDS             26..382
                     /locus_tag="ACHLAG_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP0656896.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(433..1329)
                     /gene="yhaJ"
                     /locus_tag="ACHLAG_003752"
     CDS             complement(433..1329)
                     /gene="yhaJ"
                     /locus_tag="ACHLAG_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFP0656897.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1434..2135
                     /locus_tag="ACHLAG_003753"
     CDS             1434..2135
                     /locus_tag="ACHLAG_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFP0656898.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2158..2322
                     /gene="yhaL"
                     /locus_tag="ACHLAG_003754"
     CDS             2158..2322
                     /gene="yhaL"
                     /locus_tag="ACHLAG_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFP0656899.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2534..3844)
                     /gene="cyuA"
                     /locus_tag="ACHLAG_003755"
     CDS             complement(2534..3844)
                     /gene="cyuA"
                     /locus_tag="ACHLAG_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFP0656900.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDSTAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWHGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3872..5203)
                     /locus_tag="ACHLAG_003756"
     CDS             complement(3872..5203)
                     /locus_tag="ACHLAG_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0656901.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5478..6842)
                     /gene="tdcG"
                     /locus_tag="ACHLAG_003757"
     CDS             complement(5478..6842)
                     /gene="tdcG"
                     /locus_tag="ACHLAG_003757"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP0656902.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFTRIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6914..7303)
                     /locus_tag="ACHLAG_003758"
     CDS             complement(6914..7303)
                     /locus_tag="ACHLAG_003758"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFP0656903.1"
                     /translation="MKKIIETQRAPSAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7317..9611)
                     /gene="tdcE"
                     /locus_tag="ACHLAG_003759"
     CDS             complement(7317..9611)
                     /gene="tdcE"
                     /locus_tag="ACHLAG_003759"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFP0656904.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREIDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKSGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9645..10853)
                     /gene="tdcD"
                     /locus_tag="ACHLAG_003760"
     CDS             complement(9645..10853)
                     /gene="tdcD"
                     /locus_tag="ACHLAG_003760"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFP0656905.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10879..12210)
                     /gene="tdcC"
                     /locus_tag="ACHLAG_003761"
     CDS             complement(10879..12210)
                     /gene="tdcC"
                     /locus_tag="ACHLAG_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFP0656906.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12232..13221)
                     /gene="tdcB"
                     /locus_tag="ACHLAG_003762"
     CDS             complement(12232..13221)
                     /gene="tdcB"
                     /locus_tag="ACHLAG_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFP0656907.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13320..14258)
                     /gene="tdcA"
                     /locus_tag="ACHLAG_003763"
     CDS             complement(13320..14258)
                     /gene="tdcA"
                     /locus_tag="ACHLAG_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFP0656908.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3718 a         3849 c         3355 g         3432 t
ORIGIN      
        1 cacttttaat taaagggatg tttttatgca gtggtattta gccgtattaa aaaattatgt
       61 tggtttctct ggtcgcgcac gtcgtaaaga gtactggatg tttactctga ttaacgccat
      121 cgtcggcgct attatcaatg tcattcaatt gattttaggt ttagagtttc cattcctttc
      181 tctcatttac ctggcagcaa caatcattcc ggtcattgct ctgtgtgttc gtcgtctgca
      241 cgatactgac cgttcaggtg cgtgggcgct tctctatctg gtaccgatca ttggctggtt
      301 agttctgttt gtatttgcct gtttagaagg caattctggc agcaatcgtt acggaaacga
      361 tcctaagttt ggttcaaatt aatcttagaa ttggggcgac atttcgcccc tttttattaa
      421 taataacaaa tattattttc cgctaaaaag tttgggaatt tcccgcagac accaggattt
      481 cgcttccccc atactgtcac gacgccaggc cataataata tcgatctcgc tggtcgattc
      541 cgggctgacg acacgcaacc gcccttccgc aatatctttt tcgaccatcg gatacggcat
      601 cgtcgccacg ccaagccccg ccagtaatgc ctgacgttta tcttcaatcg tgctcaccgt
      661 taagcgcggc tgtttgtcca gcagctgtac ggtcaacacc gggcgctcac gagcggtatc
      721 cgctaccgca attccacgat atttcacgcg cgtcacttca gataacggtt ccggctcctg
      781 atgaatcggg tgatcaggcg cggcaacgta gacgttcatt aaggtataga gcttgcgcga
      841 gttgatctcc gacgaggaac gaaaatgcat atccggcgcg ataacgatat ccgcccgccc
      901 ctgctccagt cgttcccacg cccccgccag cacttcggtg atgattgcca gttgggtatt
      961 ggcttttgcc gccagtttgt cgattaacgg gaaaaaggca ggtgtcggta ccagcgcctc
     1021 ggtcacaatg gtgagatgcg tttcccaacc gcgcgcgagg gcttccgcat cggtagtcag
     1081 tttatctgcg gcttccagca aaacgcgccc tcgctccagc aacatcctcc cgacattggt
     1141 gaatttggtg cgatggcccg agcggtcaaa cagcaccaca tccagctctt cttccagttt
     1201 ttgcatggtg tagctaagtg cggaaggcac gcgtcccagc tcatccgccg ccgccgcaaa
     1261 actgccccgg cgatcgatcg catccataac ccgtagtgct tccagcgtta atgccctttc
     1321 tttggccatt tcgttctcat tcaggaaatt tgaacatacc gggcagaata tctggctaac
     1381 aatgtagcgt ccagcccctt accataaaag gaagtaaaga gaggtcaaga attatgatta
     1441 ctacccgaac tgccagacag tgtggacaag cagactacgg atggttgcag gcccggtata
     1501 ctttttcctt tggacactac ttcgacccga aattgttagg ctatgcctcc ctgcgcgtgc
     1561 ttaaccagga agtgctggcc ccaggtgccg cctttcagcc gcgaacctat cccaaagtcg
     1621 atattttaaa tgtgattctg gatggagaag cagagtatcg cgacagcgaa ggcaatcatg
     1681 ttcaggccag cgccggtgag gcgttgctgc tctctaccca gccgggtgtc agctacagcg
     1741 aacacaatct cagcaaagac aaaccgttaa cacgaatgca gctttggctg gacgcctgcc
     1801 cgcagcgaga gaatccgctg attcaaaaac tggcgcttaa tatgggcaag cagcaattaa
     1861 tcgcctcgcc agagggtacg atgggaagcc tgcaattacg ccagcaagtg tggctgcacc
     1921 atatcgtgct cgacaaaggc gaaagtgcga atttccagtt gcatgggcca cgcgcgtatt
     1981 tgcaatcgat tcacgggaaa tttcatgcgc ttacgcatca tgaagagaaa gcggcgctga
     2041 cctgcggtga tggggcgttt attcgtgacg aggctaacat tacgctggtt gccgattccc
     2101 cactgcgcgc tttgctgata gatttgcctg tctagttgtt tttacaggga gatgatgatg
     2161 agtaaaaaat cggccaaaaa gcgccagcca gtgaagcccg tggtggcgaa agaacctgct
     2221 cgcaccgcca aaaattttgg ctatgaagag atgttgagcg agctggaagc catcgtcgcg
     2281 gatgctgaaa cgcgtttagc cgaggatgaa gctaccgcgt aaagacaggt tgccggatgc
     2341 ggcgaaaacg ccttatccgg cctacaaaat ccaacaacat taacgaatta aacaacccgt
     2401 aggccggata agatgcgcca gcatcgcatc cggcatcagg tacaaaacac aacaacatca
     2461 acaagttgag caccccgtag gccggataag gtgcgccagc atcgcatccg gcacaatact
     2521 caaaacctgg cgtttatctg gccttgctcg ccataatctc gataatctgc cgatccgttt
     2581 gctgcatcga atggcttgct aacgcacaca ggttggcaat cgactgctca acatcatgcg
     2641 ccacgatccc ttcattgccg gtcacggcgg tatcatccag cgccattaac accgctttcc
     2701 acgcagccga agcactggtc gaaaccttca tcgcgcagct gttcgacgca ccatcgcaaa
     2761 tcatgccgct gacatcgccg atcatactgc tgatcgccat cgagatggtt tcataacgcc
     2821 catccaccag ccatgccatc ccggcggcgg cccccattgc tgcggtcgtt gcggcacaca
     2881 gcgcagacaa acgcggtaac tggttatgga tgtaaattgc gctcaaatga gaaagcatca
     2941 gcgcacgcgc cagccgttca tcatccgctc cgaagtgttc tgctaccacc accacaggca
     3001 ttgttgcggt gatcccctgg ttacccgagc cggagttact catagccgga agcgtagcgc
     3061 cgcccatacg cgcatcggat gccgcgctgg tacgaatcac aatggatgaa gagagatctt
     3121 tcgccagcaa accgcgctcg cactgttttt ccagcgtcgc gccaatatgc agcccccact
     3181 taccgctcaa accttcctgc gataacgcac aatttagctt cgcggaatcg agaataaagc
     3241 ggatcgccgc aaacgggact tcattgacga acttcaggat ctcagccagc gtcgttctgg
     3301 aaagcaccgt cagcggagac tcttgctcgc cctctgccac acacgcctgc tgggtaaaca
     3361 ccacaccatt gtgcgtctca atatgcacaa tgttggtatg cccgccgacg atggtgacac
     3421 acgcccactt ctcaccgtgc cagactttgg cgcgtgagaa gaggatttca ttgcaaggtt
     3481 cctggatctt aacggagact ttccccgccg ccagcagtgc tttggcatcg gcaattgcct
     3541 gcgcagttga gtctttcagc acttccagcc cggcgttggc atttccacct aacgccccca
     3601 gcgccgccgc aatcggcagc cccaccattc ccgtgccggg aacggtgacg cccagaccgt
     3661 tcttcatcag atttggcgaa acccaggctt ctacacgttc aaccggacct tccagttctg
     3721 ccgcagcaac cgccgccgcc agcgccagtg aaatcggttc agtacatccc agcgccggtt
     3781 ttacttcctc ctgaactgca aggatgtaac gctgccataa cggatttaaa gtcgaatcaa
     3841 acatattaaa aaccttaaaa tttcaggtaa atcaagaaaa tgccaggaac ggagaaacac
     3901 aaagcaacaa accagtgacg ataatcaggt acagagacat tcctttgtat ttgtgcaatg
     3961 ccggtacttt gtaaaccagc cacgccggga tcaggcaccc taccatgccg aaaatcgggc
     4021 tacagataga ggtgaagctc aacaccggtg cgttcagtac gatggcgctc caggccagca
     4081 aaatggcgaa aatcatgatg ccgcgctgaa caagattttc gttaatcttc tcggcaggca
     4141 tcttgcgacg caggatgttc attacgatcc cttgcgttgc ttcgcgaaag cctaaataga
     4201 cgccaaagaa tgcagtcatg acagcaaaga tattgagaat gacgctgacc actttcaccc
     4261 acgctgcgcc gtcaccgcta ataaactgtg cggcaatcgc cagcgcggaa atattctgct
     4321 cataggcttt taccgcttcg tcatgtccca tcgccagcgt gaacgacacg gcgtagaaaa
     4381 agacggtgac aaacaaaatg ccaaacgcga tattcattgc ccgcaatgct ttatgccgcg
     4441 ccacttcaat tgatttttcc cgcgagcgat aagagatcac catcgggctt aacgtctgga
     4501 taaacagaat cgacgtcagg gtaaacggca gcgtaataat ggcgtttttc accagcagcc
     4561 ccagcggcgg tagcgaaccg acgttataca gatgccacat tcccaccatc gacacgccca
     4621 gcgccgcgac caccagcagc ttggtcagca ccatgccggt cgaaattttg aataacaatt
     4681 tctcgccgcg tgaagagatc gccaccagaa tgcaaatcag taccagacca taaaagggac
     4741 tgtctgacag caacccttcc gtcacgccga aggtatgcag ataggaagca ctatcgttgg
     4801 tgatggcggt ggaatagacg aacatccaaa tcaccagcat tacgaaatag agcgcaccta
     4861 acaggatgcc ccagttttta cctaaataac cgctaatgac actcgggtaa tctttacatt
     4921 ctggtgattc tgccagcgta ttaataaaca accgctgaaa cagatacatt gccgggtaac
     4981 caatcaccga tgagagcaaa aatacccaca atcccatcaa accgacctgc accgggagaa
     5041 aaacaatccc cgcgccaatc gccatcccga tactcataat cacccagccg gtgtcagtac
     5101 tgtcgaattt gatcgcttct cgccactcgc tctcactcat tccagcacga cccgccgggg
     5161 tcgaagcgtc tgcaatgacg cctttattcg atgcaatttc cataatttct cgctcaatat
     5221 tttgtagggc ttattatttt tttccgagcc gcatcaaggc gatatgcggt ctacgttttt
     5281 gcaggcgagt tattagaata aagaaatgat acgcgcgagg tcagagaaaa tcttcacaat
     5341 caagactttg aaataacgga gatggataag aattttctac taaattaatc gcaagagaat
     5401 attcattctc tatttgcggc agatcacaaa aaaaaggtgc acatttgtgc acccaaggat
     5461 gaaagctgac agcaatgtca gccgcagacc actttaatgg ccagtcctcc gcgtgatgtt
     5521 tcgcggtatt tatcgttcat atctttgccg gtttcataca tcgtctcgat cactttatcg
     5581 agtgaaacac gcggtgccga ggtgcggcgc atcgccatcc gcgcggcgtt tactgctttc
     5641 acggcattaa tggcattacg ttcaatgcac gggatttgta cctgtccggc aaccggatcg
     5701 caggtcagcc caaggttatg ctccatcgcg atttccgccg cattgcatac ctgcgccgga
     5761 ctgccgccca gtagttcagt taaccctgcc gccgccattg aacaggccac gccaatctcc
     5821 ccctgacagc cgacctccgc gccagagatg gaggcgttca ttttatacag cgcgccaata
     5881 gcccccgctg ccagaaaata gcgggcaatt gaccgctcgt ttaccggacg acggaactta
     5941 tcgtaataag ccagcactgc cggaataatg ccgcacgcac cgttagtcgg tgccgttacc
     6001 acgcgcccgc cagctgcgtt ttcttcacta accgccagcg cgtacatgtt gatccagtcg
     6061 atgacattca tcggatcgtt agagatgtta tcgctagaaa ccagctgacg acgcagcgct
     6121 acggcacgac gcggcacatt gagcggacca ggcagcacgc cttcagtgtt catcccgcgt
     6181 tcaataccgt catgcatcac ttgccagata cgggtaaacc ctgcgtcaat ttccgcttta
     6241 ctgcgcagcg ctagctcgtt gtgcatcatc aggccagata tagacaggcc gttgtaatca
     6301 cacattttca gcagttcacc tgctgagtgg aaatcgtaag gtacggacgt ttcgacatcg
     6361 tgcgacaggc cgaagtgttc ttcttcgaca ataaacccgc cgccgacaga gtaataggtt
     6421 ttacttaata gcgcttcctg tcccttccag gcagtgatcc gcattccgtt ctcatggcga
     6481 ggcaacattt cgggatggaa gataatgttc tttgctacag gaaaatcaac aatatgcgca
     6541 ccagatgcca ctggcagccg cccgctgcgc gttactaact ctataaatgc agggatctca
     6601 tcaatgacaa catcctgcgg actgtttcct gccagtccca tgataatggc gacatccgtg
     6661 gcatggcctt tgcccgtcag tgacaacgac ccatacagat cgaccacaat atggctcgtc
     6721 gcggttaata agccgctact ttccagccga tcaataaaac tttttccggc attcattggc
     6781 cccacggtat gcgaactgga gggaccaatc ccaattttga aaatatcgaa tgcactaatc
     6841 atatccacac cctcggattg ccgttcagtg aagtggagcg gaacgacctt gcgaccgtcc
     6901 cgctcacgag gctttacgca ctacgtactg cgatagcttc aatttccagc ttcacatctt
     6961 tcggcaaacg cgcaacctgc acacagctcc gggtcgggta ggtcgcctga tgctcatcga
     7021 agaactgctt atagacttca ttgatggtgg caaaatcatt cagatcggtg atgaacacgg
     7081 tcatcttgat gatatcgccc acgctcagcc cggcagcaac cacgatcgct ttgacgtttt
     7141 cgaggcttaa acgcgcctga tcttgcacat cagccgggat ctcaccggtc tgcgggcaaa
     7201 ccggaatttg cccggaggtg aagaccatgc tgcctaaatc aacgccctga acataagggc
     7261 cgattgcgct tggggcacgt tgcgtttcga taatcttttt catacatcct ccggcgtcag
     7321 agcgcctggg taaaagtacg tgaaataaca tcctgttgct gttcacgggt cagtgcgttg
     7381 aagcgcacgg cgtagccaga gacacggatt gtcaggttag gatatttttc cgggtgctcg
     7441 atggcatcca gcagcatttc ccgattcatt acgttgacgt tgaggtgttg accgccttcg
     7501 acatccgctt cgtggtggaa atacccatcc agcaggccga caaggttggt tttacgtact
     7561 ggatcttctt tgcccagcgc cgcaggaacg attgagaagg tgtacgagat cccatctttg
     7621 gcgtaggtga acggcagttt cgccaccgac gtcaatgagg ccacggcacc tttgcggtca
     7681 cgaccatgca tcgggttagc gcccggcgcg aacggtgttc cggcgcgacg accgtccggc
     7741 gtattaccgg ttttctggcc gtacaccacg ttagaagtga tagtcagaat cgactgggta
     7801 gggacggcgt tgcgataggt tggcagcgct ttaattttct tcataaagcg ttcaaccagg
     7861 tcgcaggcaa tgctgtctac gcgctcgtcg ttgttgccgt actgcggata ttcaccgtcg
     7921 atttcaaagt ccaccgctag gccgttttcg tcacggattg gtttcacgcg ggcatatttg
     7981 atggcagaca gggagtccgt cgccaccgac aggcccgcga tgccgcatgc catagtgcga
     8041 tagacatcac gatcgtgcag cgccatcagc gaagcttcgt agctgtactt gtcgtgcatg
     8101 tagtgaatga tattcagcgc gctgatgtac tgcaccgcca gccagtccat gaagtgatcg
     8161 aggctgtcca tcactttgtc gtaatccagc acgtcgtcca tcagcggtgc tgttttcggc
     8221 ccgacctgaa tcttcagctt ctcgtccacc ccgccgttaa ttgcgtagag cagcgttttc
     8281 gccaggttag cgcgtgcacc aaagaactgc atttgcttac caatcaccat tgggctgacg
     8341 cagcaggcaa tcgcgtaatc gtcgctgttg aagtcagtac gcatcagatc gtcattttca
     8401 tactgcaagg aagaggtgac gatcgacacc tgcgcggcat attttttgaa ggcaatcggt
     8461 aattcttccg accaaagaat ggtcaggtta ggttccggtg ccggccccat agtgtgcagg
     8521 gtgtgcaaat agcggaagga gtttttggtc accagcgtac gaccgtccag ccccatcccg
     8581 ccgatcactt ccgtcgccca gattgggtcg ccggagaaca gcgaatcaaa ttccggtgta
     8641 cgcaggaagc gtaccatacg gatcttcatg atgaagtgat cgatcagttc ctgtgcctgc
     8701 tgctcattga gtacgccaga tttaaagtcg cgctcaatgt agatatcgag gaacgatgcc
     8761 gtgcggccca gcgacatcgc gccgccattt tgcgatttca ctgccgccag ataagcgaag
     8821 tagagccact gcaccgcttc ctgcgcattc tgcgccgggc gagagatatc aaagccatat
     8881 ttcgccgcca tttcctgaat ctgcaacagc gcatgacgat gctctgccag ctcctcacgc
     8941 agacggatgg tggcttccag atcctcgcct ttttccagac gagactggag atcggcaaat
     9001 tgcagttcgc gttcacgtac cagataactg atgccataca gcgctacgcg gcgatagtca
     9061 ccgataatgc gcccacggcc atagccatct ggtaaaccgg tcagcacgcc agatttacgg
     9121 cagcgcagca tatccggtga gtaaacatca aatacgccct ggttatgggt tttacgcaga
     9181 tcggtaaaca gatattcaaa ttcactgtct atttcacggc cataggcgtg gaatgaactt
     9241 ttaatcatat taatgccacc gaacgggtgt agcgcacgtt tcaacggcgc atccgtttgc
     9301 aggccaacaa ttttttccag cggctggtta atatatcccg catcatgagc ggtaattgtg
     9361 gtggcaatat tggtatcgaa atcaaccggc gcgtgggttg cgttttcgat acggatacct
     9421 tccattactt tttcccacaa ttccgtggtg gcaggcgtcg cttcggcgag gaaagattca
     9481 tcgccttcat acggtgtata gttatgttga ataaaatcgc ggacattaat ttcgtttttc
     9541 cagtccgtac ctttaaagcc aagccatgcg tcggcgtaca gcttatcgct ggtatcaata
     9601 tctaccttca tgaaaaataa tctctctaca atacttcaac taaattatgc aaattctgcg
     9661 ggcgcgttaa ctttgcctaa atgaatggca tccaaagcaa tcattttttc ttcgttagtc
     9721 ggaataacag cgcaaatgac acgcgcattt tcactggaaa caattcgctc accacaggag
     9781 ttagagcgat tattcatttc tgtatcaatc tctacgccta atacagccaa atgttccatg
     9841 accagacgac gaattaagct tgaattctct cctattccgc cggtgaatat aattccatcc
     9901 aggcgacgta atgaagctgc gtgtccggca atatgacggg caattcggtg aacaaaggtt
     9961 ttaattgcca gttgcgcgcg ttcgtgacct tcatgccagg ctttttccag aacacgtaaa
    10021 tccgaagaaa gaccggaaat acctaataat cccgactctt tattcactac gcgttccagg
    10081 tcacccaggc tctggttggt ttggctggcg acccaggaca tcgcaccaaa gtcgacatcg
    10141 ccactgcggg tacccatcat caagccttcc agcggcgtca ttcccattga agtatcaaca
    10201 ctctgaccgt tgcgaaccgc gcagattgac gcgccattgc caagatgcgc cacaaccaag
    10261 ccggagtcat cttccgccag attcagcagc gaatgtgcgc gctgggaaac atagcggtgc
    10321 gacgtgccgt ggaaaccata acggcgtaca cctaactctt cataatattt ccacggcagg
    10381 ccgtataaat aagcttccgg agccatcgtc tggtggaaac tggtatcaaa taccgccacc
    10441 tgagttacgc ccggaaataa ttgctgcgcc gattcaatac cacttaaatt ggcgtaatta
    10501 tgcaggggtg ccagtggaga aacgcgacgg atattatcaa tgacttcatc ggtaataatg
    10561 gcggactcgg taaaaatact gccgccgtga gcgatgcggt ggccaattaa ggccacactg
    10621 tcatttaaat tccgtttttc cagttcaaat gcaattgcct tcaatgcacc ttcgtagctg
    10681 tggtgagcca gcggtgctgg ctctccccca tttacggata agaatgcatt ttccgagtta
    10741 ataccgtcgg caatacctga cattaatact tcacagtcgc tggcatcgag tacggaaaac
    10801 ttaatcgaag acgaaccaca gttaataacc aaaacaaccg gaaattcatt catctctttt
    10861 ctcatcctga gttacggatt aaaacagttt gtatacgatg ttcaggatgg tcagcagacc
    10921 aatcacggta acaaacacgt tatccagacg accacggtat ttcgccagag acggcgcttt
    10981 acggatggca tacatcggca acaggcacag cagggatgcg ataatcggtg cgcccatggc
    11041 ttcaataagg tcgaggatgt tcgggttggc gtaggcaaca acccaggtgg agcccatgat
    11101 gaagatcatg ctgatagtgt tcagtttacc cagcgacact ttagttttgt cgcctttata
    11161 accgaacttc agaatcagac cattcaagcc ttccagcgtc cccagatagt gaccgaagaa
    11221 agatttgaag atagccacga gtgcgatgat ggaagccgca tattccagtg taatcgcgaa
    11281 cgttgttttg gtaccggtca tggacgcaaa gtggttagcc agataagaaa gcactggaat
    11341 attctgcgct ttggcttccg ccatgttggc cggagacagc gtaaacaggc agctaaaggc
    11401 aaagaacatc accactgcaa ccatcagcat gctggcacga gaaatgattt gggaacattt
    11461 acgttcggtg aagtcgcgac cgaagtcttt ctcatactct tcacgtttag aaaccacgaa
    11521 ggaagagacg attggcgaga agttaaagga gaaaaccatg atggaaatcc ccagccagac
    11581 agtgatcagg ataccgtcat gaccggttaa cgacagcgaa ccgaggtcaa cctggtcgat
    11641 aactgcagag ttccagtaag ggatcagcga cagagaaatc agcaccaggc tggcgataaa
    11701 cggccatacc aggtagctca tcactttaac catcagatcc ttaccaaacc agatgacgaa
    11761 agccatcagc agcaacagga acagcgccac aaagccgcga ttcagcggtg caaatccgag
    11821 ctggttttcc cagaacgtca taaaggtatt ggtaatagta acgccataaa tccacagcag
    11881 tgggcaaatc gcgaagaagt acaggaacgt gataaccacg ccgccagttt taccaaaatg
    11941 ctcttccacc gtttccgtaa tgttgccgga agggttagag ccagaaagac acagacgcgc
    12001 cagcgcccgg tggcaataaa atgcgatggg gtatgccaat accaacatca gaagaatcgg
    12061 gatcagtccg ccaaaacctg cgcggatagg gaagaacagc accccggcgc cgattgccgt
    12121 accaaacaag cctaacgtcc atgtggtatc tgatttacgc caggacgatt gttttgtctg
    12181 gctggataca atgctatctg aagtactcat atcctatcct caacgaatta attaagcgtc
    12241 aacgaaaccg gtgatttgag agacgcgaga aagatcgata ttgccgccgg aaataatact
    12301 gacggttttt ctgttttgaa tatattggtc taatttaccg cttaataatg cagcacatgc
    12361 cagagcgcct gcgccttcgg tgacgacttt attgcgctga attaaggcaa tcatactgtt
    12421 tctgatttcg tcttcgctga ccagcacgat gtcatcgact aattcacgaa cgatttcgta
    12481 agttaaatta cccgggcggg agacatcaca accatccgcc agggtgccgg tagttcggtg
    12541 cgtggttatt tctccggagt ggaaagaagc cgccatgccg tgaacgtttt ctgactgtac
    12601 gccaataaca cgaatggtcg ggttaataga tttaattgcc accgcaatac cagcaattaa
    12661 accaccacca ccaattggca caatcacgtt atcgacatca tagagatctt ccataatttc
    12721 cagaccaatc gttccctggc cagcaatcac tttcggatca tcgtaaggtg ggataaaaat
    12781 acgaccttcc atttcgacaa tttcgctcac tttagcgata gtgtcgttga aattatcacc
    12841 atgcagaacg acttctgcgg agtagtcgca cgttgccgct actttggatt ttggcgcacc
    12901 ttttggcatc accactttac cgtcgatacc cagcatcgcg caggagagcg aaaccccttg
    12961 cgcatggttg cccgcagaac aggccactac acctttacgt ttttccgcat cagtcagtga
    13021 acttaattta ttaaatgcgc cacgaatttt aaatgaaccc gtacgctgca tattttcaaa
    13081 cttcaggaat atttcacctt tgcaacgttc actaaaatag tttgagcgag gcatgcctgt
    13141 tttataaatt cgcccagcca gtcgttgttt cgcttcaata atgtcatcaa tagcaaccgg
    13201 cagatcgtat gtaatatgca ttataaaacc tcttcgcctg aattaaatat gtaggtaacc
    13261 gtaaccgaca cctgcaaaac agacaggtgg attatttatg tttaaataaa aacaaataac
    13321 taaccaactt ctattaattg ccttcgtcta cacccattat aagatgaata ctctttggct
    13381 aattccacca aaaccgatgc tgctttttta atacgataat ttttcgacca taccgcggca
    13441 tattgtgcca caggtaatgt ttcttcaacc ggaatagtaa taaattgatt tgaaccaaaa
    13501 ggtgacgtca tatcacaagg aattacagtt aagaaatcag cattgagaac aagattataa
    13561 attgtcacga ctgagtcggt tttaacgatg ttttcaatac tgatgccatt tctttgtaac
    13621 gtagtaagaa gctcgctgta gtaccccata ttggtttgtg gcaacaccca ctgttcgttc
    13681 ttcaacgact ccagcgtggt ggtgccggtg catgttcggg acttactggc taccaacaca
    13741 aactcggact cgaacagcgg ctcaacatgt aaatcctgaa gcttcatttc tgcacttaac
    13801 gtaccaatcg caaaatccag tcgaccgtcg cggattgccg gtaagaacga agacagttgc
    13861 gcttcataca tagaaacctg cgctttcggg aacacctctt tgaacttgtt gatcatccct
    13921 gacataaaag taaaaccaat caatgaagga aaaccaaatg agacttccac caccgcctca
    13981 gaagacatac cgcttatctc attaaccata tttttcattt cacgggtaat ggattcggaa
    14041 cgggagagta acaattgacc ggcaggtgtt aatgttacac cggtattttt ccgcaccact
    14101 aattccacac caaaataatc ttcaatatcg ttaatgattt tactgacggc tggttgagtt
    14161 aaccctaatt cttttgcagc cgagccgata gagccacttc taatgacttc ctgaaagact
    14221 accaggtgct gcgttttcgg aagaagaata gtgctcataa cgacctacgt taattacctc
    14281 attgacggca tgaagtgtat caaaatgaaa tgaacaggat atgtacgacc actcacaaat
    14341 taactttcaa tact
//
LOCUS       DAZGVQ010000125        14340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000125 DAZGVQ010000000
VERSION     DAZGVQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.126"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(123..533)
                     /gene="ybcV"
                     /locus_tag="ACHLAG_003764"
     CDS             complement(123..533)
                     /gene="ybcV"
                     /locus_tag="ACHLAG_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309653.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HFP0656909.1"
                     /translation="MAQAAIFKEIFDQVRKDLNCELFYSELKRHNVSLYIYYLATDNI
                     HIVLENDNIVLVKGLKKVVNVKFSRNKHLIETSYNKLKSKEITFQQYRENLAKAGVFR
                     WVTNIQEHQRYYYAFDNSLLFTESIQKTTQILPR"
     gene            834..1040
                     /locus_tag="ACHLAG_003765"
     CDS             834..1040
                     /locus_tag="ACHLAG_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656910.1"
                     /translation="MNKEQSADELSLDLIRVKNMLNSTISMSYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNSKDYSLVVD"
     gene            1594..2088
                     /locus_tag="ACHLAG_003766"
     CDS             1594..2088
                     /locus_tag="ACHLAG_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFP0656911.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPAAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            2088..4190
                     /locus_tag="ACHLAG_003767"
     CDS             2088..4190
                     /locus_tag="ACHLAG_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP0656912.1"
                     /translation="MLNQETAKAARTDSGYILRAPRRMRVADAVAQYMRVPMGAGNSV
                     PWDPLVAPYVIEPMNCLASREYDAVIFVGPARTGKTIGLIDGWVIYNVICDPADMLII
                     QMTEEKAREHSKKRLARTFRVSPEVVSRLSPNKNDNNVYDRTFLAGNYLKIGWPSVNI
                     MSSSDYKCVALTDYDRFPEDIDGEGDAFSLASKRTTTFMSSGMTLVESSPGRDVKDVK
                     WRRTSPHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPCGDVVAGFRDIADPVLAS
                     EAAYIQCPSCSGRILPEQKRELNGRGVWLRDGESINADGSRYGDPRRSRIASFWMEGP
                     AAAYQTLSQLVYKLLTAEQEYETTGSEETLKTVINTDWGLPYLPRASMEQRKSELLEQ
                     RAEPVPSRSVPDGVNFLVATVDVQAGRHRRFVVQVTGYGSRGERWIIDRYNITQSLRG
                     DSDGESQRIDPASYPEDWDVLLTDVFHKSWPLASDPSQQMRLMAMAVDSGGEDGVTDN
                     AYKFWRRCRRDGLGKRIYLFKGDSIRRAKLITRTFPDNTGRTGRRAQAAGDVPLWLLQ
                     TDALKDRVNNALWRDSPGPGYVHFPDWLGSWFYDELTYEERSSDGKWSKPGRGANEAF
                     DLMVYAEALVILHGYEKIRWPDAPEWASRETWLECVPDSTEPSPTPEPVSTPVKKQKR
                     KKTVTDDVNPWLTSGGWL"
     gene            4187..4399
                     /locus_tag="ACHLAG_003768"
     CDS             4187..4399
                     /locus_tag="ACHLAG_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656913.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            4327..5907
                     /locus_tag="ACHLAG_003769"
     CDS             4327..5907
                     /locus_tag="ACHLAG_003769"
                     /inference="COORDINATES: protein motif:HMM:TIGR01539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0656914.1"
                     /translation="MGAPPCGSDYTTTGASRVPAGEVLMAILDDVIGVFSPGWKAARL
                     RSRAVIQAYEAVKTTRTHKARRENRTADQLSQYGAVSLREQARYLDNNHDLVIGVFDK
                     LEERVVGKNGIIVEPHPVLRNGAIARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLM
                     LRTWLRDGEVFAQMVSGRINSLTPSAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDD
                     WGRPEKYLVYKSRPVSGRQMETKEVDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSAL
                     KEYEDSELTAARIAAALGMYIRKGDGQSYETDGNDSKENERELTIQPGIIYDDLKPGE
                     EIGMVKSDRPNPNLETFRNGQLRAVAAGSRLSFSSTARNYNGTYSAQRQELVESTDGY
                     LILQDWFIGAVTRPMYRAWLKQAVASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKE
                     AEAWKIQIRGGAATESDWVRAGGRNPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGS
                     SAATKRQEPQHTDDQSEE"
     gene            5852..7879
                     /locus_tag="ACHLAG_003770"
     CDS             5852..7879
                     /locus_tag="ACHLAG_003770"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFP0656915.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENTFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDEPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            7966..8289
                     /locus_tag="ACHLAG_003771"
     CDS             7966..8289
                     /locus_tag="ACHLAG_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HFP0656916.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGMTEGVFILPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            8282..8557
                     /locus_tag="ACHLAG_003772"
     CDS             8282..8557
                     /locus_tag="ACHLAG_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFP0656917.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMAVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            8569..9147
                     /locus_tag="ACHLAG_003773"
     CDS             8569..9147
                     /locus_tag="ACHLAG_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0656918.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPLSGPLTQ
                     AFEDARDHIIAAEMPKQLGYALKQQLRLWLTR"
     gene            9144..9545
                     /locus_tag="ACHLAG_003774"
     CDS             9144..9545
                     /locus_tag="ACHLAG_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP0656919.1"
                     /translation="MNRHTQIRQAVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDIPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            9556..10299
                     /locus_tag="ACHLAG_003775"
     CDS             9556..10299
                     /locus_tag="ACHLAG_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0656920.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVS
                     VTGLTVSPASASVKVGATTTLTFTVKPDGASDKAISVHSSDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTADGNFVAVTAVTVSAAG"
     gene            10360..10746
                     /locus_tag="ACHLAG_003776"
     CDS             10360..10746
                     /locus_tag="ACHLAG_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP0656921.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAGEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNADQSKDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGAIDNAVDDEHEALTPEKS"
     gene            10755..11084
                     /locus_tag="ACHLAG_003777"
     CDS             10755..11084
                     /locus_tag="ACHLAG_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP0656922.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            11056..14121
                     /locus_tag="ACHLAG_003778"
     CDS             11056..14121
                     /locus_tag="ACHLAG_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0656923.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEVQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRLNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSASARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQIAALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNDLQEKKRQKDLQDAKEQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKALASGKKKTRETRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQDFTVDADNTAETAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFSPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            14121..>14340
                     /locus_tag="ACHLAG_003779"
     CDS             14121..>14340
                     /locus_tag="ACHLAG_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0656924.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCEHPSLKAFLERHGGVRAFQW"
BASE COUNT         3536 a         3236 c         4212 g         3356 t
ORIGIN      
        1 cctcgttttt acggggtttt gttatatttg aatggttccg aatatctaaa tcacaattgt
       61 tgatggtttt tattaaacca atgcagtccg gctcaggagt gagagaagcc ggacgttatg
      121 gtttagcgtg gtaagatctg tgtagttttc tggatgcttt cagtaaatag taatgagtta
      181 tcaaaggcat agtaatatct ttggtgttcc tggatatttg taacccatcg gaaaactcct
      241 gctttagcaa gattttccct gtattgttga aatgtgattt cttttgattt caacttatta
      301 taggaggtct ctataagatg tttgtttctg gagaatttaa catttacaac ctttttgagt
      361 ccttttacta acactatgtt gtcgttttct aacacaatgt gaatattatc tgtggctaaa
      421 tagtaaatat aaagtgagac attgtgacgt tttagctcag aataaaataa ttcacagttt
      481 aaatctttac gcacttgatc gaatatttct ttaaaaatgg cagcctgagc cattggtaaa
      541 ccttccatgt gatacgatag cgcgtagttt gcattatcgt gtttattgtt tcaatctggt
      601 ctgacctctt tgtgttttgt tgatgattta tgtcaaatat taagcctgtt tttaatgaat
      661 agtgttgatt gcgtaacaaa gttgagcctt gctggcatcc aggagggata tgcaaccgac
      721 agatgtatgt aaggtcgatg tactcaaact ttcatacttt tcctctttta tgcagaaaga
      781 tttgaagtaa tattttaacc gctagatgac gagcaaacgc atggagcgac aaaatgaata
      841 aagaacaatc tgctgatgaa ctctcgttgg atctgattcg tgtaaaaaat atgcttaata
      901 gcaccatttc tatgagttac ccggatgttg taattgcatg tatagaacat caggtgtctc
      961 tggaagcatt cagggcaatt gaggcagcgt tggtgaagca cgataagaat tcgaaggatt
     1021 attccctggt ggttgactga gcaccataac tgctaatcat tcaaactatt tcacctgtga
     1081 cagagtcaat gtcgcattct gtcactgtca tgctaataca gagctgcaat tcaactactg
     1141 caatgtcctc gtaattaggt gaatttacaa tatcgtcctg ttcggatgcc ggctgcattg
     1201 ctgaagatga ggcatttatg gttcgcatat tttcccctca tgctcgtcag tcctgtgcgt
     1261 aggaagaaac aggacactca cactaatttg tgtgggcatg ctgtgatgtc cttctgaatt
     1321 attcctatgc cattatgtaa agcgctgtat cagatgctcg tcacggctgt caggctgtcg
     1381 ggtcctcccg gtggggcccc tgccacgggg cgggagcgtc gcggaaaaag gctagttttt
     1441 gaaattttat tcgtcatcac cactactgta attgattgat attacagttg ttttattttt
     1501 atggtgtcga ttctgattgt tttttgttca tcactaacac cgtttgccta aagttgttcg
     1561 caagatgcat gtttaaaaca ttctggagcg ggtatggatc gagagttaaa aaatctgacg
     1621 ctgaatatca gtcaactggc ggcattgtca ggtgtacatc gccagactgc tgcggcaagg
     1681 ctgcaaaatc tacccgttgc aggggggcat gaaagcaacc tcaagcttta tcgggtggtt
     1741 gatattgtgt cggcatttct ggcattacca ccgccggttg cagaaggcga aatggacgcg
     1801 catgagcgca aagcctggta tcagtctgaa cgtgagcgtc ttaagttcga acaggaaacg
     1861 gcacaactca ttccggccag tgatgtcaga cgggagtttg ccatctgggc aaaagcggtc
     1921 gtgcaggtgc tggagacatt accggatatt cttgaacgtg actgcggtct gcagcctgcc
     1981 gctgtgagcc gtgttcagtc cattattgat gatctgcgcg atcagatagc cctgcgggtg
     2041 actgaagcag gtgcggatga tgaggaggaa ttacagcagg aggagtaatg ctgaatcagg
     2101 aaaccgcaaa ggcagcacga accgattcag gttatatcct tcgcgcaccg agacgaatgc
     2161 gggttgctga tgccgttgct cagtatatgc gggtgcccat gggggcaggg aactcagtcc
     2221 cgtgggatcc gctggtggca ccgtatgtta ttgagcctat gaactgcctg gcctcgcgtg
     2281 aatacgacgc agtgatattt gttggcccgg cacgaaccgg caagactatc ggcctgattg
     2341 acggctgggt gatttacaac gtgatttgcg atcctgctga tatgctgatc attcagatga
     2401 cggaggaaaa agcccgcgaa cactccaaaa aacgactcgc cagaacgttt cgcgtcagcc
     2461 cggaagtggt cagtcgcctg agtccgaaca aaaatgacaa caacgtttat gacagaacat
     2521 tccttgctgg caactacctg aaaatcggct ggccgtcagt caatatcatg tcttcatcag
     2581 attataaatg cgtggcgctg acggattatg accgttttcc ggaagatatt gatggtgagg
     2641 gggatgcctt ctctcttgcc tcaaaacgta ccaccacatt tatgtccagt ggtatgacgc
     2701 tggtggagag ttcccccggc agggatgtga aggatgtgaa atggcgacgg acttcaccgc
     2761 atgaggctcc accaaccacg gggatcctgt cgctctataa ccgtggcgat cgccgtcgct
     2821 ggtactggcc ctgtccacac tgtggtgagt attttcagcc ctgcggcgat gtggttgctg
     2881 gtttccgtga tattgccgat cctgtgctgg caagtgaggc ggcttatatt cagtgtcctt
     2941 cctgttcagg acggattttg cctgaacaaa aacgcgagct gaacggacgt ggggtctggt
     3001 tacgggatgg tgaatccatc aatgcagatg gcagtcgtta tggtgatccc cggcgttcac
     3061 gtattgcgtc attctggatg gaggggccgg cagctgctta tcagacactc tcgcaactcg
     3121 tttacaaact gcttactgca gaacaggaat acgagacaac cggaagtgaa gagacactca
     3181 agacggttat caataccgac tggggattac cttatcttcc ccgcgccagc atggagcaac
     3241 gaaaaagtga attgcttgag cagcgggcag agccagttcc ttcccgcagt gtgccggatg
     3301 gcgttaattt ccttgtggcg acagtggatg tgcaggcggg acgtcatcgc cgttttgtgg
     3361 ttcaggtaac gggctatggc agccgtggcg aacgctggat tattgatcgt tacaacatca
     3421 cgcagtcatt gcgcggtgac agcgacgggg agagccagcg aattgatccg gccagctatc
     3481 cggaagactg ggatgtcctg ctgacggatg tttttcataa aagctggccg ctggcctccg
     3541 atccttctca acaaatgcga ctgatggcaa tggcggtgga ctccggcggt gaagacgggg
     3601 tcactgataa tgcctataaa ttctggcgtc gttgccgtcg tgatggcctt ggtaaacgta
     3661 tttacctgtt taagggcgac agcatccggc gcgcaaaact gatcacccgt acattccctg
     3721 ataacaccgg acgaacgggc cgacgggcgc aggccgcagg tgatgtaccg ctctggcttc
     3781 ttcagacgga tgccctgaaa gaccgggtga ataacgcgtt atggcgtgac tcgccaggtc
     3841 ccggctatgt gcatttccct gactggctgg ggagctggtt ttacgacgaa ctgacgtatg
     3901 aagagcggag cagtgacggg aaatggagta agccgggtcg cggtgccaac gaagcttttg
     3961 acctgatggt gtatgccgag gctctggtca ttctgcatgg atacgaaaag atccgctggc
     4021 cggatgcacc ggagtgggcg agccgggaaa cctggctgga gtgtgtcccg gacagtaccg
     4081 aaccgtcacc cacaccggaa ccggtatcca cgcctgttaa aaaacaaaaa cggaagaaaa
     4141 cagtaactga cgatgttaac ccctggctga cttccggagg atggttatga atcagaatga
     4201 tattgaagcc atgattcagc gttatacgga agctgaaatg gcagtgctgg acggaaaatc
     4261 cgtcactttt aatggtcagc agatgaccat ggaaaactta tctgagatcc ggcaggggcg
     4321 gcaggagtgg gagcgccgcc ttgcggctct gattacacga cgacgggggc atcccgggta
     4381 ccggctggcg aggttctgat ggcaattctt gatgatgtga ttggcgtttt ttcaccagga
     4441 tggaaagcgg caaggctgcg ttcccgagcg gtgatccaag cttatgaggc cgtaaaaacg
     4501 acgcggacac acaaagcccg acgggagaac cgaactgccg accagttaag ccagtacggg
     4561 gccgtgtcgt tacgtgagca ggcccgttac cttgataaca accacgatct ggtcattggt
     4621 gtatttgaca agctggaaga acgggtggtg gggaaaaacg ggattattgt cgagccacat
     4681 ccggtattac gcaatggtgc cattgcccgt gacctggcag cggagatacg tacccgatgg
     4741 agtgaatggt ctgtcagtcc ggaagtcacc gggcagttta cccgtccgat gctggaacgt
     4801 ctgatgctgc gtacctggct gcgcgatggt gaggtgtttg cccagatggt ttccgggcgc
     4861 ataaacagcc tgacgccttc tgccggtgtt catttctggc tggaggcgct cgagccggac
     4921 tttattccca tgaccagtga tgagagcaac aggctgaatc agggcgtgtt tgttgatgac
     4981 tgggggcgtc ccgaaaaata tctggtgtat aaaagtcgtc ctgtatccgg acggcagatg
     5041 gaaaccaaag aagtggatgc agagcgaatg ctgcatctta aatttgttcg ccgtctgcac
     5101 cagatgcgcg ggacgtcttt attgtccggt gtgctgatcc gcctcagcgc cctgaaagag
     5161 tatgaagatt ctgagctgac tgcagcaagg atcgccgctg ctctggggat gtacatccgg
     5221 aaaggcgacg gacagagcta tgaaacggat ggtaatgaca gcaaggagaa tgaacgcgag
     5281 cttaccattc agccaggcat tatttacgac gatctgaaac ccggcgaaga aatcggaatg
     5341 gtgaagtcgg atcgtcccaa tcctaacctt gaaacttttc gtaatggtca gttgcgtgcc
     5401 gtggcggcgg gcagtcgtct gagtttttcc agtacggcac gcaactataa cggcacttac
     5461 agcgcccagc gtcaggaact ggttgagtct actgatggct acctgatcct gcaggactgg
     5521 tttattggtg ccgtcacccg cccgatgtat cgtgcctggc tgaaacaggc tgtggcatcc
     5581 ggtgttatca ggctaccccg cgatcttgac cgttcttcac tgtataccgc ggtgtattcc
     5641 ggaccagtga tgccgtggat tgaccctgtt aaggaggctg aggcctggaa aatccagatt
     5701 cgtggtggag cggcgacaga atcagactgg gtacgtgctg gtggtcgtaa tccggatgat
     5761 gtcaaacgtc ggcgcaaggc cgaaattgat gaaaaccgca agctggatct ggtatttgat
     5821 accgatccgg ccagtgataa aggaggcagc agtgccgcaa cgaaacgaca ggagccgcag
     5881 cacaccgacg accagtccga agaataattc ctggttcagg atgcaggctg gtcaccagag
     5941 tgacgcggat atttatattt atgacgagat tggtttctgg ggtgttacag cgaagcagtt
     6001 tatcagtgat ctgaatgcac tgggcgatat cacccacatt aatctccata ttaattcacc
     6061 gggtggcgat gtctttgaag gcatcgccat ttttaatgcg ctgaaaacac atggtgcgtc
     6121 cattaccgtt tatgtcgacg gtgtggcggc gtcaatggcg tcggtcattg cgatggtggg
     6181 aaacccggtc attatgccgg aaaacacttt catgatgatt cataaaccat ttggctttac
     6241 gggcggtgat gcggaggaca tgcgcaccta tgccgacctg ctcgataaag ttgaggcggt
     6301 tctgttaccc gcttatgcac agaaaaccgg gaaaaccacc gatgaaattg ctgccatgct
     6361 ggcggatgag acctggatgt ccggtgccga atgtctggca catggatttg ctgatcaggt
     6421 aacgccagcc gttaaggcaa tggcatgtat tcagtcaaaa cgtacagagg aatttaaaaa
     6481 gatgccggaa tccattcgaa acatgattac tccgccacgc aacagtgctc cacgcgtaca
     6541 ggatgatgaa cctgcagcct cccggacgcc agtgcaggca gcagcacccg tggtggatga
     6601 aaacagtatc cgtgcgcagg tactggcaga gcaaaaagcg cgtgtaaacg gtattaatga
     6661 tctgtttgcc atgtttggcg ggcgttatca gactctgcag gctcagtgtc ttgccgatcc
     6721 tgaatgttcg ctggagcagg cccgcgaaaa gctgttgaac gagatggggc gcgagtccac
     6781 gccatccaat aaaaataccc cggctcatat ttatgccggt aacggtaatt ttgtggggga
     6841 cgggatccgc caggcgctga tggcgcgtgc cggatttgaa aaaaccgaac gtgataatgt
     6901 ctacaacggg atgaccctgc gtgaatatgc ccgtatgtca ctgactgaac ggggtattgg
     6961 ggtttccagt tataacccga tgcagatggt cggtgcggcg ttcacacaca gtacgtctga
     7021 cttcggtaat attctgctgg atgttgcgaa caaagccatt ctgcagggct gggaagatgc
     7081 tcctgaaacc tatgaacagt ggacgcggaa aggtcagttg tctgatttta aaattgccca
     7141 tcgtgtgggt atggggggct tcagtgctct gcgtcaggtg cgtgaagggg cggaatataa
     7201 atacgtcacc accggagata aacaggccac tattgcactg gcgacctatg gcgagctgtt
     7261 cagtatcacc cgtcaggcca ttatcaatga tgatctgaat atgctgaccg atgtcccgat
     7321 gaagctgggc cgtgcggcga aatccaccat tgccgatctg gtttatgcca ttctgacgtc
     7381 taacccgaaa atctccacag ataatgtaag tctgttcgat aaagcgaaac atgcaaacgt
     7441 actggagagc gctgcaatgg acgtggcatc gctggataaa gcccgccagt tgatgcgcgt
     7501 tcagaaagag ggggagcgtc atctgaatat tcgtcctgcg ttcgtactgg taccgacggc
     7561 gatggagtct gttgctaacc aggtcattcg ctcctcaagt gtcaaggggg ctgacattaa
     7621 cgcaggtatt attaacccgg tgaaagattt tgcgaccgtt attgcagagc ctcgtcttga
     7681 tgataacagc cagaccacct tctacctggc tgcgtccaaa ggctccgata cgattgaagt
     7741 ggcttatctc aacggtgtgg atacgccata tattgatcag atggagggct tcagtgtgga
     7801 tggcgtgaca acgaaagtgc gtattgacgc cggtgtcgcg ccagttgatc accgcggtct
     7861 ggtgaaatgt acggcgtaaa cgtcgcagac aacaactctg atggcccgta agggcttttt
     7921 ttgtacctga aatcagcctc tgaacggggc tgtacggaga cagttatggc aaagaatttt
     7981 gtagaagaag gaaaaacggt ggcgattgtt gccagtgcag ccatcagcag cggagatctg
     8041 gtgcaggtgg gtgatgtttt tgcggtggcg ctgaccgata ttccacaggg tgaaacaggc
     8101 gacggcatga ccgaaggtgt gtttatcctg cctaaactga aaacggatga catgaaaacg
     8161 ggtaagaagg tttatctgaa gtccggaaaa gttcagctga ctaacagcgg ctctgatccg
     8221 ctggtcgggg ttgtctgggc agatgccgga accagtgcag aagaagtgcc ggtaaaactc
     8281 aatgtctgat cccttttccc ggctggcagc gcgtatggat gcgatcacgg tcagaaagat
     8341 gggaaagaca gcctcgatta atgatgtcga tatggctgtg atcccgggcg aaacactggc
     8401 agagctgaat gctctgtccg gacctgcggt ctctctggtg gtgttttctt cgggataccg
     8461 cccacggcgc ggggatcgcg ttgtttatga cggacaacaa tggacggtca cacggcatga
     8521 acgttttaac ggtaagccaa tgatctttat tgagtaaaga ggtgtgggat gaaggggctt
     8581 gagaatgcca tccgcaatct gaacagcctt gatacccgta tggtgccaca ggccagcgca
     8641 tgggcgataa accgtgtggc acagaaagcg gtctcggttg ccacccggca ggttgccggg
     8701 aataccgttg cgggagataa ccaggtgaaa gggatccccc tgaaactggt acgtcagcgt
     8761 gtccgggtgt ttaaagccag tccgtcagga aaaatgacgg ccaggatccg cgttaaccgg
     8821 ggcaatctgc ccgccattaa gctggggaca gcccgggtca gactggcccg gcgtggtgga
     8881 aaactgcagt accgtggcag tgtgctgaag gtgggtaaat atcttttccg ggatgcgttt
     8941 attcagcaac tggcgaatgg tcgctggcat gtgatgcggc gtattgatgg caaaaatcgt
     9001 taccccattg atgtggtgaa aatcccgctg tccggaccgc tgacacaggc atttgaagat
     9061 gcccgcgacc acatcattgc tgcggaaatg ccgaaacagc tggggtatgc actgaaacaa
     9121 caactgaggt tatggctgac ccgatgaacc ggcatacaca aatccgccag gccgtactgg
     9181 cacgccttcg ggaacagtgt ggagacagcg ccacgttttt tgacgggctt ccggcattta
     9241 ttgatgcgca ggaactgcct gccgtggcgg tgtggctgag tgatgctcag tacaccggaa
     9301 aaatgacgga tgaagatgac tggcaggctg ttctgcatat tgccgtcttc atccgggcac
     9361 aggcaccgga ttcagagctg gatatgtgga tggagagcac cattttccct gccctgaatg
     9421 atataccggc actttccgga ctcatcgaca ccctgatccc actcggtttt aactatcaac
     9481 gtgataatga gatggccacc tgggcgatgg cggaaatcac gtaccagatc acgtacacga
     9541 attaaggagg tggtaatgac cacaccaaat ccactggcaa agacgaaagg tgcggggacg
     9601 acgttctgga tgtataccgg caacggcgat gcgtttgcga accctttgtc ggacactgac
     9661 tggctgcgtc ttgcgatggt gaaggatctg caacctggcg aaatgaccgc tgatgcagaa
     9721 gatgacactt atctcgatga tgaagatgca gactggaaaa cgacaaccca ggggcagaaa
     9781 tccgttggtg atacttcggc gacgctggcc tggcgtccgg gtgacagcgg gcagaaaaaa
     9841 ctggttcagt tgttcgactc cggtgaagtc tgcgcgtttc gtatcaaata tcccaacggc
     9901 actgttgatg ttttccgtgg ctggctgagc tcactgggta aaaccattgc ctcaaaagac
     9961 gtgatgaccc gcactgtgaa aatcagcggt gtggggcgtc cgtatctggc agaggaaggc
    10021 actgaaaccg tgagcgttac cgggctgaca gtatcaccgg cgtctgccag tgtaaaagtg
    10081 ggagcaacca ccacgctgac ctttacagta aaacctgacg gagccagtga caaagcgatc
    10141 agtgtgcatt cgtcagatcc acagactgcc acggttaccc tgaacggact tgtggccacg
    10201 gtaaaaggcg tgaagcaggg cagtgtcagc attgtgggca tgaccgctga cgggaatttt
    10261 gtggctgtga ctgcggtgac tgtcagcgca gcaggttaac aggacgatac tcatcatttg
    10321 ccccggttat gcggggcttt tttgcaggtg gagaacatga tgtttctgaa acaggacacg
    10381 tttaattatg aaaaacagtc cgtggtgctc agtgagctgt ccgggctgca gagaattgaa
    10441 tatctgacgt ttgttcagca gcgaacggca aagtttgatg ccggggaggg agaactgccg
    10501 gaggctgaac gacagattgc ttttctgcgg atggggatgg atatcaatgc ctggctggtt
    10561 tcccgctcac tgtggaatgc ggatcagtct aaggatgtag agacgctttg cgcatccgtt
    10621 attacaacat ggtcgtatga tgccctgggt gcgggggcgg agatggttct gtcgctgagc
    10681 ggtatggggg ccattgataa tgccgtggat gatgagcatg aggcgctgac gccggaaaag
    10741 tcctgacgcg ggaaatgcag tttgtcatgc ggcttgcccg ggagttccgg cgggcagact
    10801 ggcggcggat gctgtcggaa atgtcggcca ctgagcttgg tgagtggggc gattatttcc
    10861 ggatgcagag cttcagtgat gtgtggatgg atgcgcagtt tgcctcgctg aaggcattga
    10921 tcgtgagaat ggtgtccggc agcagtgatg ctgcggtggc tgatttcagc cttttaccgg
    10981 aagagaacgg gataccggag cgaacggacg aagaactgat gcatcttggg gaaggtattt
    11041 ccggaggtgt gcgttatgga ccagatagcc aacctggtca ttgatttggg gattgatgcg
    11101 gcagagttta aaaatgaaat tccccgtatc aaaaaccttc tgaatggtgc agccagcgat
    11161 gcagaacggt cttctgcccg tatgcagcgt tttatggagc gtcagactca ggccgcccgg
    11221 cagacaacgc aggcagcttc ttcggctgca acagccgcat ccgtccatgc gcagacggtg
    11281 gagaagaacg cacaggctca tgaacgcatg gcccgcgagg tggagaaaac ccgccagcgc
    11341 atggaggcac tgagccagaa aatgcgcgag gaacaggcgc aggccatggc tctggcggag
    11401 gttcaggata aagcggctgc tgcgttttat cgtcagattg acagtgtgaa acaggccagt
    11461 gcggggctgc aggaattaca gcgtattcag cagcagatcc gacaggccag aaacagtggc
    11521 gggattggtc agcaggatta tctggcgctg atttctgagg ttactgcgaa aacccgtgtt
    11581 cttacacagg ctgaggaaga ggctacccga cagaaagtgg cgtttatccg tcagcttaaa
    11641 gagcaggcaa cccgcctgaa tctttcatct tctgagttgc ttcgtgctaa ggcagcccag
    11701 ctgggggtaa gcagtgctgc agaagtgtat atccgcaaaa tggagcaggc aggaaaagcc
    11761 acgcattcgc tgggtctgaa aagtgcatca gcccgccagg agataggcgt tctgataggt
    11821 gaactggccc gcggcaattt aggggcgctg aggggatccg ggataacgct ggctaaccgt
    11881 gccggatgga tagacacact gatgtcaccg aaaggcatga tgcttggcgg ggttattggc
    11941 ggtattgccg cggccgtcta tggtctgggt aaagcctggt atgacggtca gaaggagggg
    12001 gaagaattta accgccagtt gtcgctgacg gggcattatg ccggagtcac tgccgggcag
    12061 ctgtggacgc tcagtcgtgc tatttccggg aatggtatca cgcaacatgc tgcagccggt
    12121 gcgctggctc aggtggtggg gagtggtgca tttcgtggaa acgatatcgg tatggtggcg
    12181 agagctgccg cacagatgga gcgatcggtt ggccagtcgg tcagcgatac cataaatcag
    12241 tttaagcggc tgaaggatga tcctgtaaat gccgcgaagg ctctggacaa tgagctgcat
    12301 tttcttactg ccactcagct tgagcagata cgcgtccttg gggagcaggg gcggtccagt
    12361 gatgctgcac ggatagccat gtctgcactg gcagaggaaa ccggtcggcg tactgcggat
    12421 attgataata acctcaatgc gcttggcagt acgctgaagt atctgtctga tttgtggagt
    12481 cgtttctggg atgcggccat gaatattggt cgtgaagact cgctggatga acagattgcc
    12541 gctttacagg agaaagtgtc gcgggcgaaa agactcccct ggacggcatc atcttctcag
    12601 gttgaatacg atcagcagcg tcttaacgat cttcaggaga aaaaacgcca gaaggatttg
    12661 caggatgcaa aagagcaggc agagcggaat tatcaggagc aacagaaacg ccgtaatgct
    12721 gaaaatgctg cactgaaccg gatgaatgaa acggaagcag cacgacatca gcgtgaaatt
    12781 gcgcgtatta atgccatgca gtacgccgat caggctgtca gggatgcggc gatacaacgt
    12841 gaaaatgaac gttacgagaa agccctggca tccggtaaga aaaaaacacg cgaaacccgt
    12901 aatgatgagg ccacccggtt attgctgcag tacagtcagc aacaggcaca ggtggaagga
    12961 cagattgctg ctgccagaca gtcagcaggc attgccacgg aaaggatgac agaagcgcat
    13021 aaacagcttc tggctctgca gcagcgcatc agcgacctgg acgggaaaaa acttacggca
    13081 gatgaaaaga gtgtgctggc ccgtaaagat gaactgattc aggcactgac gctgctggat
    13141 gtaaaacagc aggagcttca gaaacagacg gcactcaacg agctgaagaa aaaaacaatt
    13201 cagctgacca gtcaactggc tgaagaagag cgcgctcagc gtcagcaaca tgacctggat
    13261 atcgccacgg tgggtatggg tgatcagcag cgacagcgat atcaggtaca actgagtctt
    13321 cgccagaaat accagcaaca gctggagcag ttgaggcggg atagtgagca gaaagggaca
    13381 tataacacgg atgactacag aaaggccgag caggcgctga cggagagcct gaaccgacaa
    13441 ctgaatgaga atcgccgtta ctggcaacag cttgaagttg tgcagggtaa ctggaaaaac
    13501 ggagtcctgc gtgcatttca ggattttacc gtggatgcag ataatacggc agaaacagca
    13561 gaacaggtgt tctcgtcagc cttcagcaac atgggaaatg gcctggcaac ttttgtcact
    13621 accggcaaac tcaatttcaa atccttcacc tcttctgtgc tgtcagatat ggcgaaaatc
    13681 ctggcgcagg caaccatgat gaaatcgata aaagggattg gcagtgtact gggatttgat
    13741 ctcagcagcc tttccctgaa tgccaatggg gggatttatc agtctgctga tttgagtcgt
    13801 tacagtggca cggtggttaa ccgtccgacg ttttttgctt ttgcaaaagg cgcgggtgtg
    13861 atgggggaag cgggacctga agccattctg ccattgcgtc gtggtgctga cggtaagctg
    13921 ggggttgtgg cggatattgg gggttcaggt atggcgatgt tttccccgca gtacaacatc
    13981 gagatcaata acgatggcac gaacgggcag ataggtccgg ctgccctgaa ggcggtttat
    14041 gacctcggga aaaaagcggc agcggacttt atgcaacagc aggcccgtga tggtggtcgg
    14101 ttaagtggag catatcggta atggagacgt ttcactggaa agtgcgcccg gatatgaatg
    14161 tggtatcaga gccgaaagtg gtgacagtga agctgggcga tggttatgaa cagcgtcgtg
    14221 cggcgggact gaataaccag ttgtcgactt acagcgtgac gatacgtgtt cgtaaatgtg
    14281 aacacccatc tttaaaagcc tttctggaac ggcacggtgg cgtccgtgca tttcagtgga
//
LOCUS       DAZGVQ010000126        14301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000126 DAZGVQ010000000
VERSION     DAZGVQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.127"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..441)
                     /locus_tag="ACHLAG_003780"
     CDS             complement(<1..441)
                     /locus_tag="ACHLAG_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFP0656925.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGII"
     gene            699..1304
                     /gene="yahD"
                     /locus_tag="ACHLAG_003781"
     CDS             699..1304
                     /gene="yahD"
                     /locus_tag="ACHLAG_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFP0656926.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            1344..2207
                     /gene="yahE"
                     /locus_tag="ACHLAG_003782"
     CDS             1344..2207
                     /gene="yahE"
                     /locus_tag="ACHLAG_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP0656927.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            2197..3744
                     /gene="fdrA"
                     /locus_tag="ACHLAG_003783"
     CDS             2197..3744
                     /gene="fdrA"
                     /locus_tag="ACHLAG_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP0656928.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            3744..5162
                     /gene="yahG"
                     /locus_tag="ACHLAG_003784"
     CDS             3744..5162
                     /gene="yahG"
                     /locus_tag="ACHLAG_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP0656929.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEKQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            5305..6255
                     /gene="yahI"
                     /locus_tag="ACHLAG_003785"
     CDS             5305..6255
                     /gene="yahI"
                     /locus_tag="ACHLAG_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFP0656930.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            6265..7647
                     /gene="yahJ"
                     /locus_tag="ACHLAG_003786"
     CDS             6265..7647
                     /gene="yahJ"
                     /locus_tag="ACHLAG_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP0656931.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGSTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTCVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDILPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            7946..8356
                     /locus_tag="ACHLAG_003787"
     CDS             7946..8356
                     /locus_tag="ACHLAG_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP0656932.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            8610..9593
                     /locus_tag="ACHLAG_003788"
     CDS             8610..9593
                     /locus_tag="ACHLAG_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0656933.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVESGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            9642..11126
                     /locus_tag="ACHLAG_003789"
     CDS             9642..11126
                     /locus_tag="ACHLAG_003789"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP0656934.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            11119..12090
                     /locus_tag="ACHLAG_003790"
     CDS             11119..12090
                     /locus_tag="ACHLAG_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0656935.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            12087..13043
                     /locus_tag="ACHLAG_003791"
     CDS             12087..13043
                     /locus_tag="ACHLAG_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0656936.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            13130..14179
                     /gene="yahK"
                     /locus_tag="ACHLAG_003792"
     CDS             13130..14179
                     /gene="yahK"
                     /locus_tag="ACHLAG_003792"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFP0656937.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         3491 a         3410 c         3955 g         3445 t
ORIGIN      
        1 aattatcccc acgcagttac ccgccagaaa acccagtaac gtcaccgtcc agttcatccc
       61 gctggcaaaa aacagcgtca tgccgagaaa gccaccggga ataaaggaca gtaaccagaa
      121 acgcccttgc cagaccacta ccgccgagaa gggtacggta gtaattacgc tcgcccagaa
      181 cgcggacagt ccacttacag aggccagcca accggcagcc agcgcggcga aaaatgccca
      241 caccatcccg gaataattaa ccgccagact tctgataaac cccgatttac cgccgcctgc
      301 ggcataaaaa ctgcaaaagg cgacgaagcc aatggttcct aataattccc atccctgaga
      361 gagcccgaca tgtgaagaca ctaactgcca aagccctgca caaataccga cggtgacacc
      421 cgttgccgtg agtccgttca tcttgttccc ctctggttag acatggacga aagctactct
      481 tacatgtgca gactatagag aaataaaacc tgattgagtg aaagggtatc gggtcaaaga
      541 aacaaatatt gcactaccgc acactgcgaa aagattgttg aacatcattc aacaaaaagg
      601 cgaaatacag cgaattaaat ttaagcgggt tcttttatca gattatcgtt gctattctca
      661 attcatcgaa aacagccaca aaaaccaggg gaatgtgaat gagtataaaa aatctacctg
      721 ccgactattt attggctgca caacagggtg atatcgataa agtaaaaacc tgccttgcgc
      781 tgggtgtcga tattaatacc tgcgatcgtc aggggaaaac ggcaattacg ctggcaagtt
      841 tatatcagca atatgcttgc gttcaggcat taattgatgc cggagcggat attaataagc
      901 aagatcatac ctgtttaaat ccttttttaa ttagctgtct gaacgatgat ttaacgctac
      961 tacgaattat tttaccggct aaacccgatc ttaattgcgt aacccgtttt ggcggtgtcg
     1021 gcctgacgcc tgcctgtgaa aaaggccatt taagtattgt aaaagagctt ttggcgcata
     1081 cggagattaa cgttaaccag accaaccatg taggctggac gccgctactg gaagcgattg
     1141 tgcttaatga tggtggtatt aaacagcagg cgattgtgca gttattgctg gaacacggtg
     1201 ccagcccgca tctgaccgat aaatatggca aaacgccact ggaactggcg cgggaacggg
     1261 gctttgaaga gattgcgcag ttactgattg ccgcaggtgc ataaaccggg aggcttgcta
     1321 tcaacacacc agaaagacgg tgtgtgtggg cgctaactgc ggatgcggat tttctggcgc
     1381 agcgggggca aggacaggtt gaacaggtct ttgccagagc ggtaaatatc gcactcccgg
     1441 ctcgccagca gttgctgacg ctgctttgtg aagagtacga caatgcgcca aacagttgtc
     1501 ggttggcact cactcacttt gatgatctgt tccggcatgg tgataaggtt cagtttgacg
     1561 atcaaggtat tacggttggt caacatcttc atatagagat gagtcgttgt cggcgttggc
     1621 tgtccccaac cttgcaaatg accgctgtga attttcacct tatcgcctgg ctacagtggc
     1681 acgacattat tcatcagcac ctgggggaaa atgaaaccct gtttaattat cgcggcgata
     1741 atccgtttta tcaggcgtta aataaagaac tacatattaa acgacgggca gttattcagg
     1801 cagtaaacga aaaacaaaat atcgccgcag cggtcgccag tatgattggg ttagggattg
     1861 gccttacgcc atcagccgac gattatttaa caggtctggc gcttatttta tttattcccg
     1921 ggcatccgtc ggaaaaatac aaagaggaat tttatctcgg tatgcagcgc ggcagaaata
     1981 acaccacatt attaagtgct ataacgctgg aagccgcatt acaacaacgt tgccgggaaa
     2041 atattcatcg ttttattcac aacattattt atgacatccc tgggaacgca actcaggcaa
     2101 tagaaaaaat taaacatatt ggctccagtt ccggctgcga catgctgtat ggcatggccg
     2161 atggttgtgc gctgagccaa acctacggag ggaattatgt cagttaaaat agtcattaaa
     2221 ccgaatacct attttgattc tgtctcgctg atgtctatct ccacgcgtgc aaataaactc
     2281 gacggcgtcg agcaggcatt tgtggcgatg gcgaccgaaa tgaacaaagg tgtactgaaa
     2341 aatttaggac tgctgacgcc ggagctggaa caggcgaaaa acggcgacct gatgattgtc
     2401 atcaatggta aatcgggtgc ggacaacgag cagttgctgg tggagattga agaactgttc
     2461 aacaccaaag cgcaaagcgg ctcgcacgag gcgcgttacg ccactattgc cagcgccaaa
     2521 aagcatatcc cggaaagtaa cctggcggtg atttcggtca acggtctgtt tgccgctcgc
     2581 gaagcgcgtc aggcgctgca aaacgatctc aacgtgatgc tgttttccga taacgtctca
     2641 gttgaagatg aactggcgct caagcagttg gcccacgaaa aagggctgct gatgatgggg
     2701 ccggactgcg gcacggcgat tattaacggc gcggcgctct gttttggtaa cgccgtgcgt
     2761 cgcggcaata tcggtattgt tggcgcatcc ggcaccggca gccaggagct gagcgtccgc
     2821 attcatgaat ttggtggcgg tgtttcacaa ctgattggca ccggcgggcg cgacctgagc
     2881 gagaaaatcg gcggcctgat gatgctcgac gccatcggga tgctggaaaa cgatccgcaa
     2941 actgaaatta tcgcactgat ttcaaaacct cccgcgcctg cggtggcccg caaagtgctg
     3001 gaacgcgcac gtgcctgccg caagccggtg gtggtctgct tcctcggtcg cgttgaaacg
     3061 ccggtggatg agcaggggct acagtttgcc cgcggcagca aagaggcggc gctaaaagcg
     3121 gtgatgctct ccggcgtgaa acaggaaaat ctcgacctgc atacgcttaa ccagccgttg
     3181 attgctgatg tgcgtgcgcg tctgcaaccg cagcagaaat acattcgtgg cctgttctgc
     3241 ggcggcacgc tgtgcgacga aaccatgttc gcggtgatgg aaaaacatgg cgatgtctac
     3301 agcaacattc agcccgatcc ggaattccgc ctgcaagata tcaaccgcag catcaaacac
     3361 accttcctcg actttggcga tgacgacttc acgaacggca agccgcatcc aatgattgac
     3421 cccaccaacc gcatcagtcg cttgatcgaa gaggcgcgcg atccagaagt ggcggtgatc
     3481 gtgatggatt ttgtgctcgg atttggatcg catgaagatc cggtcggctc caccatcgag
     3541 gcgatcaaag aagcgaaagc gatcgccgct gccgaaggac gcgagttgat cattctcgcc
     3601 tatgtgctgg gtaccgatct tgatacgcca tcgttagaac aacaaagcca gatgctgctt
     3661 gatgccggag tgattctggc gagcagcagc accaataccg gattgctggc gcgtgaattt
     3721 atctgcaaag gggaggaagc ctgatgagcc agtcactgtt tagccaacca ttgaacgtta
     3781 ttaacgtcgg catcgccatg tttagcgatg acctgaaaaa gcagcatgta gaagtgactc
     3841 aactcgactg gacgccgccg gggcagggca atatgcaggt ggtgcaggcg ctggataaca
     3901 ttgccgattc gccgctggca gacaaaatcg ccgccgctaa ccagcaggcg ctggagcgca
     3961 ttatccagtc gcatccggtg ctgattggtt ttgatcaggc gattaacgtg gtgccgggca
     4021 tgacgccgaa aaccattctt cacgccgggc cgccgatcac ctgggaaaaa atgtgcggcg
     4081 cgatgaaagg cgcggtcacc ggggcgttgg tgttcgaagg gctggcgaaa gatctcgacg
     4141 aggcggctga actggcggcc tccggggaga tcaccttctc gccgtgccac gaacacgact
     4201 gtgtgggatc gatggcgggt gttacctctg cctcgatgtt tatgcacatc gtaaaaaaca
     4261 aaacctacgg caacatcgct tacaccaata tgagcgagca gatggcgaag attttgcgta
     4321 tgggagccaa cgatcagagc gtgatcgacc gcctcaactg gatgcgcgat gtgcaggggc
     4381 caatgctgcg cgacgcgatg aaaattatcg gcgagattga tctgcgctta atgctggcgc
     4441 aggcactgca tatgggcgat gagtgccata accgcaataa cgccgggacg acgttgctga
     4501 ttcaggcgct gacgccggga attattcagg caggttattc cgtggagaaa cagcgcgaag
     4561 tgtttgagtt tgtcgctagc agcgactact tctccggccc gacgtggatg gcgatgtgta
     4621 aagcggcgat ggatgcggcg cacggcatcg aatacagcac cgtggtcacc accatggcgc
     4681 gtaacggcgt cgagttcggc ctgcgggtca gcggattacc ggggcaatgg tttaccggtc
     4741 cggcgcagca ggtcatcggc ccgatgtttg ccggttataa gccggaagat tcggggctgg
     4801 atatcggcga cagcgccatc actgaaacct acggtattgg cggatttgct atggcgaccg
     4861 cgcctgcaat cgttgcgctg gtgggcggca cggtggaaga ggctatcgat ttctctcgcc
     4921 agatgcgtga aatcactctc ggtgaaaacc ccaacgtcac cattccgctg ctcggtttta
     4981 tgggcgtgcc gtcggcaatc gacatcaccc gcgtgggtag cagcggcatt ctgccggtga
     5041 tcaacactgc catcgcccat aaagatgcgg gcgtcggcat gattggcgcg ggcattgtgc
     5101 atccaccttt tgcctgcttc gagaaagcca ttcttggctg gtgcgaacgt tacggcgtct
     5161 gacctacatg ttcatgccgg atgcggcgtg aacgccttat ccggcctacg gatggcccga
     5221 gaattgtagg cctgataagc gtagcgcatc aggcagtctg gcgttggtca taacctaatc
     5281 accctctgta gcggacataa caacatgaaa gagcttgtgg tcgttgccat tggtggcaac
     5341 agcattatca aagataacgc cagccagtcg attgagcatc aggcggaggc ggtgaaagcc
     5401 gtggccgata cggtgctgga aatgctggct tccgattacg acattgtgct gacccacggt
     5461 aacgggccgc aggtcgggct ggatttacgc cgtgcggaga ttgcccacga gcgcgaaggg
     5521 ctgcccttaa cgccgctggc gaactgtgtg gcagatacgc agggcggcat cggctatctg
     5581 atccaacagg cgctgaataa ccggctggcg cgtcacggcg agaaaaaagc cgtcaccgtg
     5641 gtaactcagg tggaagtgga taaaaacgat ccaggttttg cccatcccac caagcccatt
     5701 ggtgcattct tcagtgaaag ccagcgtgac aaactacaaa aggcaaaccc tgactggtgt
     5761 tttgttgaag atgccggacg gggctatcgc cgcgtggtcg cctcgccgga accgaaacgt
     5821 attgtcgaag cacccgccat taaggcgctg atccaacaag gttttgtcgt aattggcgcg
     5881 ggcggcggtg gaattccggt agtgcgtact gaagcggggg attaccaaag cgtggacgcg
     5941 gttattgaca aagatctctc taccgcgctg ctggcccgtg aaattcacgc cgacattctg
     6001 gtgatcacca ccggcgtcga aaaagtctgt atccactttg gtaaaccgca gcagcaggcg
     6061 ctggatcggg tggatattgc caccatgact cgctatatgc aggaagggca ttttccgccc
     6121 ggcagcatgt tgccaaaaat catcgccagc ctgacgttcc tggagcaggg cggcaaagaa
     6181 gtgattatca ccacgccgga atgcctgcct gcggcgctgc gcggtgaaac gggcacccat
     6241 attattaaaa cgtaaggacg taggatgaaa gaaagcaata gccgccgtga atttctgagc
     6301 cagagcggta agatggtcac cgccgccgcg ctgtttggta cctctgtgcc gctcgcccat
     6361 gcggcggtag ctggcaccct aaactgcgaa gcgaacaaca ccatgaaaat cactgacccg
     6421 cattactatc tcgataacgt gctgctggaa accggttttg actacgaaaa cggtgtggcg
     6481 gtgcagaccc gcacggcgcg ccagaccgtg gagattcagg acggcaaaat tgtcgccctg
     6541 cgcgagaaca agcagcatcc ggacgccacg ctgccgcact atgacgctgg cggtaagttg
     6601 atgctaccca ccactcgcga catgcatatt catctcgaca aaaccttcta cggcgggccg
     6661 tggcgctcgc tcaatcgtcc ggcaggctcc actatccagg acatgatcaa gcttgagcag
     6721 aaaatgctgc cggagttaca accgtatacg caggagcggg cggaaaaact gatcgatcta
     6781 ttgcagtcga aaggcaccac cattgcccgc agccactgca atatcgaacc ggtttccggc
     6841 ctaaaaaatc tgcaaaattt gcaggcggtg ctggcgcggc gtcaggcggg cttcgagtgt
     6901 gaaatcgtcg ccttcccgca gcacggtttg ctgctgtcga aatcggaacc cttaatgcgc
     6961 gaagcgatgc aggcgggtgc gcattacgtc ggcgggctgg acccgacctg tgtcgatggc
     7021 gcgatggaaa aatccctcga caccatgttt cagattgcgc tggactacga caaaggtgtc
     7081 gatattcacc tgcacgaaac cactccgtcg ggcgtggcag ccatcaatta tatggttgaa
     7141 acggtagaga aaacgccgca actgaaaggt aagctgacca tcagccacgc ctttgcgctg
     7201 gctacgctca acgaacaaca ggtcgatgcg ctggcgaatc ggatggcggc gcagcaaatt
     7261 tctatcgcct cgacggtgcc gattggcacg ctgcatatgc cgctcaaaca gttgcacgac
     7321 aaaggcgtaa aagtgatgac tggcactgac agcgttatcg accactggtc gccttatggt
     7381 ctgggcgaca tgctggaaaa agccaatctc tacgcgcagc tctatattcg tcctaacgaa
     7441 cagaacctct cccgttcgct gtttctagcc actggcgata tattgccgct gaatgaaaaa
     7501 ggcgagcgtg tatggccaaa agcgcaggat gatgccagct ttgtgctggt ggacgcctcc
     7561 tgttccgccg aggcggtggc gcgtatctcg ccgagaaccg caacgttcca taaagggcaa
     7621 ctggtgtggg ggagtgtggc aggttgatgc gcatttgaaa cgcctgcaac ggtgaattgt
     7681 aagagattta agccccaggg agtggggcgg gaatggtttg ctaatgaatg atcctgaact
     7741 ttataatctc gcggcataat ttccagggga ttatttgaga gaaatacaca aaagtgagtt
     7801 ttaacaaagg tatttatcag gccataaatg tgacctgctt cggataaaaa agattcagta
     7861 tcgaaaaacc aatgatactc ttgctgatgg ttaccaacag taattaagat gtgatttgtt
     7921 tttccgtaat cgcaaaaata ttgccatgac cataaaagaa aaattctcac aaaaatatcc
     7981 ccatgcttcc ttttgtactt ttggcgattc agcagcgttg gccgatcacc tcgcaacgtt
     8041 gattgcgact ggtgttaaaa cagcttcctg tggttcgctg gcgggatgta ttgaagacaa
     8101 cgcatttccg atgattggtg agtataagat tgtagaaaat agccgtggtg agcctgtatg
     8161 tgttatcaga gtgattggcc tgcatttact gcgcttttct gatgtcaccg tggagcttgc
     8221 ccgtaaggaa ggtgaaggtg acctcagtct tgaatattgg cgtaatgaac accgtcgttt
     8281 tttccaggca gaaggaagct attcaccaga aatggacgtt atatttgaag aatatgccct
     8341 tattgacgtt gtgtagataa ctatttatct acgcgaaata aactcacaca cataaaatat
     8401 cgctaatttt gtttattttg ctatttttta cgtgatgaat gtcacactta ttgttgctgc
     8461 ccgttttgtc catatttata accatatttt taatagctgc ctgaaatagg gctgtttatt
     8521 ctgcataaaa atattcgaaa attcttattc tctccagcgc gctaatttgg gcgaacactt
     8581 cctgactacc ctgcaatgag gctgaaatga tgaataaacg ttttgttatc aatatggtgt
     8641 cttcgctgtt attaggcgct gcgctaattt ctgcgccgct ccaggcagcg gaaaaagtcg
     8701 tggtgaatat ttctaaagtc gatggtatgc cgtggtttaa tcgcatgggc gaaggtgtgg
     8761 ttgagtcggg taaagccttt ggtgttaatg cctcacaggt ggggccttca agtactgatg
     8821 ctccgcaaca agttaaaatt attgaggatt taatcgcccg taaggttaat gcaattacta
     8881 tcgtgccaaa cgatgccaac gtgctggaac cggtattcaa aaaagcgcga gatgccggaa
     8941 ttgtggttct gaccaacgaa tcccccggtc agccaagtgc taactgggat attgaaatca
     9001 ttgataacga aaaatttgcc gctgaatatg ttgagcatat ggcgaaacgc atggggggca
     9061 agggcggcta tgtgatttac gtgggtagtt tgaccgtgcc gcagcataac ttatgggctg
     9121 acttgctggt gaaataccag aaagagcatt acccggatat gcatgaagtt acccgccgca
     9181 tgccggttgc cgagagtgtg gatgactcac gtcgtaccac actggacctg atgaaaacct
     9241 atcctgactt gaaagcggtt gtctcctttg gttcaaacgg tccgattggc gcgggacgtg
     9301 cggtgaaaga gaaacgggcg aaaaataaag tcgcagtcta tggcatgatg atcccttcac
     9361 aggcggcatc actgattaaa agtggtgata tcaccgaagg gattacttac gatccggcct
     9421 ctgcaggtta cgcactcgca gccgtcgcca gcacgttgct gaagggtgaa gagatcaaac
     9481 ctggattaga aatgcagaat ctgggtaagg ccgacgtaga tatggataag cgaattatcc
     9541 gtttccataa ggtattactg gtcaacaaag acaatatcga ctcactttat taatccattt
     9601 cttcggggcg aaagccccga ttcttattgc caggggatgt tatggaaact ttcctttccc
     9661 ttcgtcatat caacaaaacg tttcatgcca cgcgagcgct gcgcgatgtt tctctcgact
     9721 ttatgtcggg tgaagtgcac tgccttgccg ggcaaaacgg ctgtggtaaa tcgacgttga
     9781 ttaaaattat gtccggtgtt tatcgcccgg atgagggagc ggaaattacg cttggtggga
     9841 aaaactggtc aaagctgaca cccgccgctt cggtggcgca ggggattcag gtgatttatc
     9901 aggacctctc tttatttcct aacctgagtg tctgggagaa tatcgccgtg aatcactatc
     9961 accacggtct gtttgttaac cgccgtcgtc tgcgtgaggt ggcgcaggcg gcaatgacca
    10021 gtattaacgt cacattgcca ctggatacac tcgtttctga gctttcaatt gcccgctgtc
    10081 agctggtagc aatttgccgt gcactggcgc aggacgcacg gttgatcgtg atggatgaac
    10141 caaccgcttc gctgacccat caggaagtgc aagggttatt acaggtggtg catcaattgc
    10201 gggaacgcgg gatctgcgtg gtcttcgtca gtcatcgtct ggaagaagtg atggaagttt
    10261 ccgaccgtat ttcagtgctg aaagatggtg agctggtcgg gacttttccg gctgcagaga
    10321 tgaccacaaa acaactcggt ttcctgatga ccggccagga gttcgaatat caggtgcgag
    10381 agttgtggca gggaaaatcc agcaccccgg tgcttgaggt gcgtaattta agtcgtcatg
    10441 gggaatatct aaatattaac ttgcgggtgg aagcgggcga agtggtatcg attgttggcc
    10501 tgcttggtgc ggggcgcacg gaattatgtc tgagtctgtt tggtatgacc cgaccggatg
    10561 ctggcgagat cctcatcaac ggccagccgg ttacactgca tagtaaccag gacgctatcc
    10621 gccacggtat tggttatgtt tcggaagatc gcatgtcgcg cggtttggtg atggcgcaat
    10681 ctattgaaga caacatcatt agcactgttt ttcacaaggt aaaagatcgc ttcggctttc
    10741 tgagtgaaac taaagttcgt gacctggtgg acaggctaat caaggcattg accatcaaag
    10801 tctctgatcc tcatcttccg gtgaatacgc tttctggtgg taacgcgcag cgagtatcga
    10861 tcgccaaatg gctggcgatt gggccacgac tgttgattct cgatagcccg acagtcggtg
    10921 ttgatatcgc caacaaagca ggaatatacg gcattatcag cgatctggcg gctcatggca
    10981 ttgcggtatt gatgatctgc gatgaaattg aagaagcgtg gtatcaaagc caccggattt
    11041 tggtaatgca aaaaggtcag atcacccata gcttcctgcc tgacagcagt tctcaggcca
    11101 ggattgcgga ggtggtaaat ggctgaactg aaaaaacgcc atgaattctg gctggcatta
    11161 ctgattgtgg tgctgtttgt tggccttgcc tggcgcagcg acgagtttct gacattcggt
    11221 aatttgtacg atctcgccaa taactatgcc atgttgacta ttctcgcctg tggtttgttt
    11281 gtggtgttga tttccggtgg aattgatatt tcgtttccag caatgaccat cattgcgcaa
    11341 tacggcatgg tgctgttgct gcaaaaaatt ggtggcaact tcgctgtcgc gtttgcactg
    11401 gcgggctgca tcggcatttt acttggctta attaacgcct tactggttaa tcgcctacgg
    11461 gtgccttcta tcatcatcac tatctcgacg ctgaatattt tctatggcct gctgttatgg
    11521 ttgagtaaag gtgtgtggct gtacgacttt ccgccgtggt ttgagcaggg ggttatgttg
    11581 ttcaagtaca ccgatgctga tggctatgac tatggccttg gtctgccgct gatcgccatg
    11641 attacggtgg tgctgctaac agcgtttatc atgaatttca ccagtgtagg gcgcaaaatt
    11701 tatgcccttg gcgggaaccg cgaatcagcc agtcgcatcg gctttagcgt gctgaaactg
    11761 caacttttcg tctatggcta tatgggattg atgtctggcg ctgcgggtgt agtgcagtcg
    11821 tggacggtga tgactgtcgc ccccgattct cttctgggtt atgagctgac agtactggct
    11881 gcggtggtgc ttggcggcac tagtttgctc ggcgggcgcg gcacgttaac cggtactttg
    11941 ctcggcgtgg tgttgttggc agtgatgcaa aacgggctaa atttattggg agtctcgtct
    12001 tactggcaaa cattgatcac cggcatcatc atcgttgcca gcattagtgc cacggcgtgg
    12061 agtcagcatc agaaccggag tctgctatga aaaaatcgtg gcgtaataac gttgagtttt
    12121 acttgatcgg cctgctggtg ctaactgttg ccgcgttcag cattactatg ccggagattt
    12181 tctggtcgat aagtaatttc cagtccgtcg cttcgcaaat gcccgtgctg ggcattctgg
    12241 cgttggctat ggcggtgacc atgctttgcg gtggcattaa cttgtcaatt atagccactg
    12301 ctaacgcctg ttcactggtg atggcgtggg tggcgactca gtatccaccg ggtattgcta
    12361 cggttgtcgc tacgttactg gcgggcgctg gcgcggcggt gataatcggt ttatgtaacg
    12421 gcgtattgat tgctggtatt cgagtgtcgc caattcttgc aacgcttggg atgatgacgt
    12481 tgctcaaagg gataaatatt ctggtgacag gtggtagcgc cattgccaat tatccctcat
    12541 gggtgctgtg gttgaatcat gcgcaatggt tcggtattcc gctaccgatg tggttgtttg
    12601 ccgcagtggc actggggcta tggatattgc tggagaaaac gccgctcggt aaagccattt
    12661 acctgattgg gtctaacgaa cgagcaacac tttactccgg cattaacact cgtcgggtgc
    12721 tgatttgggt ttatgtcatt tccgccttgc tttgtgcggt agcggcattt ttgatgatgt
    12781 cgaaactcaa ctcggctaag gcctcttatg gcgaatcgta cttgctggtg tcaatcctcg
    12841 cggcagtact cggtggggtt aatccggacg gcggcagcgg gcggataatt ggtatggtgc
    12901 tggcgctgtt tcttctgcaa attattgaaa gcggcttcaa tattctcggt ataagcccct
    12961 acctgacgat ggccttatgg ggaacgttgc tgctctgttt cattcaggtt cgcggcatgt
    13021 tggggctgga tcgggtggtt taaatattgg ctatattcaa tggacgcgtt ttgccgcgat
    13081 gacatatcag gcgtcgccaa atacacatag ctaatcagga gtaaacacaa tgaagatcaa
    13141 agctgttggt gcatattccg ctaaacaacc acttgaaccg atggatatca cacggcgtga
    13201 accgggaccg aatgatgtca aaatcgaaat cgcttactgt ggcgtctgcc attccgatct
    13261 ccaccaggtc cgttccgagt gggcggggac ggtttacccc tgcgtaccgg gtcatgaaat
    13321 tgtggggcgt gtggtagccg ttggtgatca ggtagaaaaa catgcgccgg gcgatctggt
    13381 cggtgtcggc tgcattgtcg acagttgtaa acattgcgaa gagtgtgaag acgggctgga
    13441 aaactactgt gatcacatga ccggcaccta taactcgccg acgccggacg aaccgggcca
    13501 tactctgggc ggctactcac aacagatcgt cgttcatgag cgatatgttc tgcgtattcg
    13561 tcacccgcaa gagcagctgg cggcggtggc tcctttgttg tgtgcaggga tcaccacgta
    13621 ttcgccgcta cgtcactggc aggccgggcc gggtaaaaaa gtgggcgtgg tcggcatcgg
    13681 cggtctggga catatgggga tcaagctggc ccacgcgatg ggggcgcatg tggtggcatt
    13741 taccacttct gaggcaaaac gcgaagcggc aaaagccctg ggggccgatg aagttgttaa
    13801 ctcacgcaat gccgatgaga tggcggctca tctgaagagt ttcgatttca ttttgaatac
    13861 agtagctgcg ccacataatc tcgacgattt taccaccttg ctgaagcgtg atggcaccat
    13921 gacgctggtt ggtgcgcctg cgacaccgca taaatcgccg gaagttttca acctgatcat
    13981 gaaacgccgt gcgatagccg gttctatgat tggcggcatt ccagaaactc aggagatgct
    14041 cgatttttgc gccgaacatg gcatcgtggc tgatatagag atgattcggg ccgatcaaat
    14101 taatgaagcc tatgagcgaa tgctgcgtgg tgatgtgaaa tatcgttttg ttatcgataa
    14161 tcgcacacta acagactgaa aaaattaata aataccctgt ggtttaacat attaacttcg
    14221 ctctccactt aactttttag ttaaggagag cgaaataata tcaaagtagc agtaaaacct
    14281 ataacgtaaa ttcaaattgt t
//
LOCUS       DAZGVQ010000127        14175 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000127 DAZGVQ010000000
VERSION     DAZGVQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14175
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.128"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1826)
                     /locus_tag="ACHLAG_003793"
     CDS             complement(<1..1826)
                     /locus_tag="ACHLAG_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFP0656938.1"
                     /translation="MLMKRHLNTCYRLVWNHITGAFVVASELARARGKRTGVAVALSL
                     AAVTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTG
                     GQWIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQW
                     VHGGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTT
                     INKNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHKYGLALNTVINEG
                     GWQVIKEGGTAAHTTINQKGKLQVNAGGEASDVTQNTGGALVTSTAATVTGTNRLGAF
                     SVVAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLA
                     DSGAAVSGTRSDGTAFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGS
                     LAGTTTLNNGATLTLSGKTVNNDTLTIREGDALLQGGALTGNGRVEKSGSGTLTVSNT
                     TLTQKTVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGATW
                     NIPDNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNG"
     gene            complement(2154..3026)
                     /locus_tag="ACHLAG_003794"
     CDS             complement(2154..3026)
                     /locus_tag="ACHLAG_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP0656939.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILLCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTTESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            4367..5857
                     /locus_tag="ACHLAG_003795"
     CDS             4367..5857
                     /locus_tag="ACHLAG_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFP0656940.1"
                     /translation="MPYFQYPDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFLLTLAESGERKTAVDKLLMEPLYQQEMLLYSRHK
                     NELTSWKNKEEILKAQKKALLSKLNKELRKGADESETLRQLEALQKNREEKPVRYKFI
                     FNDATTAAIKDQLCGQWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VTPGGSLTAFHERLMELARGSIEKSSKDERYCLHFSPEAQKIFIDHYNVLEQDLSPSG
                     PLSQFRGHVSKKTENIARIAALLQYFSQGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQLQDARELLDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNVLLNILAS
                     QLRLSVVQEGKTTYVLLSEINNITLDKLNACYSQKLIRWHEQSFGL"
     gene            complement(5979..6551)
                     /locus_tag="ACHLAG_003796"
     CDS             complement(5979..6551)
                     /locus_tag="ACHLAG_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656941.1"
                     /translation="MQKLENRCELLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSIISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(6637..6843)
                     /locus_tag="ACHLAG_003797"
     CDS             complement(6637..6843)
                     /locus_tag="ACHLAG_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0656942.1"
                     /translation="MNTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVETWLQARIAQSRP"
     gene            7700..7924
                     /gene="hha"
                     /locus_tag="ACHLAG_003798"
     CDS             7700..7924
                     /gene="hha"
                     /locus_tag="ACHLAG_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFP0656943.1"
                     /translation="MQHKVTRDEWKLKPEQFRRCSSRETLEKVSSHTRYKLTPAELEA
                     FNSAVDHRLAELIMNKLYDRVPASVWKYVI"
     gene            8559..9128
                     /locus_tag="ACHLAG_003799"
     CDS             8559..9128
                     /locus_tag="ACHLAG_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFP0656944.1"
                     /translation="MTYKYNPFWQQRIRETVLRALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKRREGKRVHPTILHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGCYATLANFPAWPVVWLERGDDTGFQ
                     QVLERADYLAKESTKAYGTGERNFGCSRG"
     gene            9295..9678
                     /locus_tag="ACHLAG_003800"
     CDS             9295..9678
                     /locus_tag="ACHLAG_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP0656945.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTEHAQQCPYVRPDRREVQLVKRLRQFVPDALPVVRKASWYCRQC
                     HHDYYGERYCTHCQTGRFSEEGGAE"
     gene            9675..10100
                     /locus_tag="ACHLAG_003801"
     CDS             9675..10100
                     /locus_tag="ACHLAG_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFP0656946.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRHCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCGRLF
                     PGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGSAST"
     gene            11636..12760
                     /locus_tag="ACHLAG_003802"
     CDS             11636..12760
                     /locus_tag="ACHLAG_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFP0656947.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLGPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
     gene            complement(12902..14173)
                     /locus_tag="ACHLAG_003803"
     CDS             complement(12902..14173)
                     /locus_tag="ACHLAG_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFP0656948.1"
                     /translation="MPMKNYLFQTPEDICVQLKKMTHPVTIRTTDIANFWHYLESATL
                     PVITKSTTTENREVTFLWRSEKALQGVYLRLNRVTDKKDVKKGLMTHIPSTDIWMLTL
                     VLPASYRGSYSFIEIPTDMTQKDIFQLGSRFSPLPGKSDPFNKTAEINIRGFGESVLS
                     LDMAPEQKEWDDTSHKCTGILSTLHSFVAGYQRRIRLYFPLNPTSVPLGLLVLPDAET
                     WFDRIDITRALDMAITTGRITPMAIMGIDNINESDRMNILGGNKELIFDIAENLIPQL
                     YRDYPDIVWAGRSNTVLAGQSLGGVTALMAAIYASTTFGTIISHSPSMWWNPDQGSPI
                     LFTENNTSWVSEQILSAPPKDVNIRLGVGSLEGTTVSHVQRLHHSLIAAGLESNLTVY
                     TGGHDYAWWRGAIIDELANYNCRKVSNNNSV"
BASE COUNT         3504 a         3301 c         3616 g         3754 t
ORIGIN      
        1 ccgttcaggt ttttcacctg cagggttgtc ggaacgaact tccctgtgcg ggcggaggtg
       61 aaatgaatct gtccggcatg gctgaggtca tccaccaccg actgaaccgt ggcgttatcg
      121 gggatattcc aggtggcacc ggatgtcagg gtgacattcg tggggtcaat ggcaccgttc
      181 agcactgtgc tgccggtcag cttcagggcc gtgccgcgct gagcgatgac atccgtggtg
      241 acggtactgt cgttcagcgt cagcgtgcct tcattcaggt tgacggtttt ctgggtgagt
      301 gtggtgttgc tgacagtgag tgtgccactt ccggattttt ccaccctgcc gttaccggta
      361 agagcgcctc cctgcaggag tgcatcacct tcacggatgg tcagggtatc gttattcacc
      421 gttttcccgg aaagggtaag cgtggcaccg ttattcagcg tggtggtgcc cgccagcgag
      481 ccccctctgg cggtgaacag tccgccattt accgtggtat ccgtggccgt atcaccggcg
      541 ttcagtgtga aggaactgcc tttttccagc atcagggcat ccgcctgacc gcctccgata
      601 cggaatgccg ttccgtcgct ccgggtacca ctgacagcgg caccggaatc ggccagcagt
      661 accccgccat tccccatgga tacggtggtg gcggcaccac cgttgcggac atccagcgtt
      721 ccgccatcat ccacacgggt attagtggct gtgtgtccgc tcagcacatc cagacgtccg
      781 ccattttcca gtacgacatt atcagcctta cccgccacaa cggagaatgc tcccaggcgg
      841 tttgtaccgg tgacggttgc agctgtactg gtaaccagtg caccgcccgt gttctgggtg
      901 acatcagacg cttccccgcc ggcattcacc tgcagctttc ctttctggtt gatggtggta
      961 tgcgcggcag ttcctccttc cttaatcacc tgccagcccc cctcgttaat taccgtgttc
     1021 agcgcaagtc cgtatttgtg cacatactgg tttcctccct ccagccgggt gttatgtgcc
     1081 tctccgtgta ccgtctggtc accgccggcg taaatgaggg tgtcccgtgc catccccgaa
     1141 gaccagataa cctgccggcc atttttgttg atggtggtgg attcagccgt ccctccgacc
     1201 atctgaccgc tggacacatt ttcagactcc ggaccacctt ctgcaccggt gttgacgatg
     1261 gttccggttg ccagtccgcc atgttttatg gtctggtaac catcctggtt aataattgta
     1321 ccggcagctt tccctccccc gtgtacccac tgctcacctc tgttatccag cgtggtgttc
     1381 accgccagtc cgttaaggct ctgccctccg ccatcacgaa taaccgtatc actggcagtt
     1441 cctcctgcct gcacaatctg acgaccattt gcggtgacag tggtgtttct gcctgtgcca
     1501 cccgctttta tccattgccc gccggtgttt tcgtcactgt ccggccccag ctcaagcccg
     1561 gtactgacag tcacgccatc agctgttccg gatacaaact ggttgtcatg gtttaccagt
     1621 gttccgccat tcactgtttc acccgggtgc acaacgatgt cagcagccag caccgggagt
     1681 gacgtgaccg cggcaagaga cagtgcaacc gccacaccgg tgcgtttccc ccgtgcgcgg
     1741 gccagttcgg aggcaaccac gaaagcgccc gtaatgtgat tccataccag cctgtagcag
     1801 gtattcagat gtcgtttcat cagcatttcc ttaatcagag tgagggtgaa taaaaaagcc
     1861 ggtatccaca gatgtggata ccggcaggta aagggttaca gtgccggagg tccggctgac
     1921 ggcagtttct gacgtgtggc tctgttgttc cctgaatctc atgtctgtgt gtatctgcgc
     1981 acgttgctaa caggagttat tattagcata acgatcgata ttaacgatcg gtgaaatagg
     2041 tatcgatcgt tttattctat tatccggcta ttgcacggac aagagagaca gtaacgaaaa
     2101 cagcgctttt gtgagcagac tcagaggcag ggaggagatc atccacaaca ggttcagaag
     2161 aagatccagt tccataccgc gcgggcaaca gagaccacgg tgtcacgaac agcgcgcagg
     2221 gccgtacgga caacagaggc aacacagacg ctctccgttg tgtcaaatat ccggtccacc
     2281 gcaccggtaa actgttcacg ggcctgcgag cggacagact ccgtgcgcag ctcgtcctgc
     2341 aggcgggtca tcaggggact ggcggcatgg tcgggaagtg ctgtcatgag cgcactgacc
     2401 agcgtatcca gttcccagcc ggtgcgggcc gatacggcca caaccggatg tacgggccgg
     2461 aacagccgga ataccgcctc tgttttctca cgaatgttct gttcctgtgc gggagaaggc
     2521 tgaataccgg ccatatccca ttcatggcag ggttccgttt tgtcggcctg cgtcaccaca
     2581 aacagtacct gctgatgtcc gcatagcagg atgtgtcgcc agaaatactc atccacagac
     2641 agggcacggt catcggcttt aatcagccac agtaccaggt ccagttcagg cagaatgtca
     2701 cggtacaggg cttcatactc tgcatccctg tcccggctct cgcccacccc gggcaggtca
     2761 gtgataacca tgctgtgacc atggccactc agacggaagc gctgcacttc ccgggtgccg
     2821 gcgtgaacat cactgaccgg tgtgacctcc ccctgaaaca gggcattaca gagtgaggat
     2881 ttaccggccc cgcttttacc cataatgcca atcacgggtt cgtgactggt gagtttacgc
     2941 agatgttcca ggatgtgacg tgaaagcgag taaggcaggg aggaaagcgg tttttcaatt
     3001 gcctcaatgg catcagacgg attcatacat gtattcccgg tgaaaaaaga caaaaccccg
     3061 cgcaccggaa aggtggcggg ggatccacgg agataatatc tgtctgaaat tgttttgaat
     3121 cgatgggatt gttgcgtatt aacatgaagg agatcaggac ttatccgtta ctgggtgatc
     3181 tccttccatg aaaatacgcg gttattgtta aacagcaggc tgattactcc gtaaaggaga
     3241 aaaatattca tcttgcagcc tgattatcag gtgtgtttat gtttactgag gtgagtattt
     3301 ttcagtatat tgtcggggca gtcagtggga gatattaaat ttacgtttag agttatcttg
     3361 cttctgctgt gtatttttat aagagtgcgc acgtaatgat atctgttgat accatgttgt
     3421 taagtgtttc ccttccgggt catgcgtact tgcaaaatgg catctgaatc gatcacggtt
     3481 tggccaaacc gtgatcgaat agagaggaat acgcatgtaa tgattaaatg cttttgttac
     3541 atcattcttc cggcatgaat atttatccag ttcccttttt tatttgtgta gtgatactgt
     3601 cggaatagcg ttcagtaatg taaagtgtcc tgttaaaaat ctttaataaa tgtgtcaccc
     3661 tggtcaggaa atatctgctg agatattatt agttggtggt gtttttactg agtaatagct
     3721 ggctttttat tcctgaactg aaatactgtc gctcgtcagg gtttaattaa aactggatga
     3781 tgatgtgaaa atgaaggctg gtttttgtgt ttgtcgagga agtgttgttt ttatccattc
     3841 tcatcattgg ctggctgcga ttatacagaa gtatttgaag aaaaaatggt gcattttatc
     3901 tgtgccggca gggaggggga ttttcctgat atgtcagtat taaatggttt ttgttattta
     3961 taaaaatgtc atcctgtcca tagaatattg tactgagtac tcagcattca gcgcaggaac
     4021 tgtcatcttc tttattttat tctccttcag gagggcagtt ttctgaaagc gttactccgg
     4081 ggaaactgtt tatgtttatt cccggcagga aacccgtacc tgcaataccc tggatgatgc
     4141 tttttgctgg tacgttgttg tgtgaaaatt aatcaacatg tgtgctccgg agagcgcccg
     4201 tagacgcaca gattcattct gacattcttt agcgacaaaa taccgagtgg ttattattta
     4261 acctgaaaat cgacagaaat ttttatcctg tattgattct gctgctattt ttgctattag
     4321 tagcaatagt tgcggtactt tatgttctgt gtgttgtgag gtcattatgc catactttca
     4381 gtatcctgat gagtttcccc tgagctcgct gcctccactc attcgtgatg ccgttattga
     4441 agctcagcaa atcacgcagg ctcccctggg gcttgtggca gcatctgccc tgggcgctgt
     4501 ttcacttgtc tgccagaatc tgattgatgt ctgcagactg aatacactac gggggccggt
     4561 ttctcttttc ttactgacgc ttgctgagtc cggagaacga aaaaccgcag tggacaaatt
     4621 attaatggag cccctgtacc agcaggagat gctgttatat tccagacaca aaaatgaact
     4681 gacgtcatgg aaaaataagg aagagatttt aaaggcgcag aaaaaagcat tgttgtcaaa
     4741 actgaataaa gaactccgca aaggcgcgga tgagtcagaa acgctcagac aactggaagc
     4801 gcttcagaaa aacagagagg aaaaacctgt aagatataaa ttcattttta atgatgccac
     4861 gacagcagca attaaagatc agctttgtgg tcagtggcgt tcagtgggta tcatgtctga
     4921 tgaagccggt ataatatttg atgggtatac cctgtctgag ctgcctttta ttaacaaaat
     4981 gtgggatggc tccgtattgt cggtggacag aaagaatgaa cccgaacaga tgattgaaaa
     5041 tgccagaatg acattatccc tcatggtaca acccggtctt tttgaccgat atatggagcg
     5101 aaaaggttct gttgccaggg acagtggttt tcttgcccgt tgtctaatca gtaaacctgc
     5161 caccacgcag gggaaacgct ttattaacgg agctgtgacc ccgggaggat cattaacggc
     5221 atttcatgaa cgattaatgg aactggccag aggcagtatt gaaaaatcca gtaaggatga
     5281 acgttattgt ctgcattttt ctcctgaagc gcaaaaaata tttattgatc actataatgt
     5341 tctggagcag gatttgtctc cttccggacc cttaagccag tttcgggggc atgtttcaaa
     5401 aaaaacagaa aacattgcga gaattgctgc gttattgcag tatttcagtc agggtgaagg
     5461 gaaaatctct gccgatatca tgacgtctgc tgtcgtaata agttcgtggt acacagatga
     5521 atataaaaaa ttatttgcgt tacctgatga atctgaactg cagctgcagg acgcccggga
     5581 gctgctcgac tggctcattg aggagtgccg cggagaatgc ccgccccgtg tcaggaaaaa
     5641 ttatattctc cagtgtggcc cgggccggtt caggaacagg aaaaagctga acgtcctgct
     5701 gaacattctt gcatctcaac tcaggctctc tgtcgtgcag gaaggaaaaa ccacctatgt
     5761 cctgctttcc gaaataaaca atatcacgct ggataaactg aatgcgtgct acagtcagaa
     5821 actgataaga tggcatgagc aatcctttgg cttataatgt tccctgttgc tgagacggat
     5881 tattcagtgg tggtatcgca gtatgcccgt caccccccgc ttctgcatga gggtgacgga
     5941 ggaaaagtaa gtgtgcggag gcccgggagc gttatgaatc aacaggcgta taacaggtac
     6001 tcgtcaatca tcagttccag ctcgcgcttt tcccgtttgc gcgccacagc ccagttttca
     6061 agctctgcat gtgatttatg ctttaactct tcagtccagg gctgcatgga gtgggcacac
     6121 atcagatcat accagcacag ccatatcagc gtgtggtaga gatcacgatc gtcgaaacgc
     6181 cggagcaggc tgttcagcct gtttgccagc cggttttcac ccagacgatg tagctcactg
     6241 agtaaaacgc cgcccagatt aaaatgtgta agcagcatga tttcatgggt ctgaatgctg
     6301 gtcattaacc cttcctgaat ggcaggaatg aacggtggtg ttcccgggtt gacgacctga
     6361 tagagtccct ggggggccac cgaaatgatg gagccttttt ccggcagaaa aaagtgtgcc
     6421 agggtcagac ggtgataacc atgaagatgc ggagacccca ttccatgagc gacgataagc
     6481 ctcgtcacgc ttgtcatcca tttctgatgc tggatgagca gcagctcaca ccggttttca
     6541 agtttttgca taatattctc tcctttagcg cgcagcaaaa ggcctccggg ggccggatca
     6601 tgcctttgct gcggttgaac ggataacggt caggaattac ggacgggact gtgcaatacg
     6661 cgcctgcagc caggtttcca cttcactttt cagccagcgg gaactgcggc ccagtttgat
     6721 gggggcagga aaggcaccat ccttgatgag cttgtaaaac catttatcgg tcaggccggt
     6781 cagttgagtg ataaatgcca tgtcgaccat ctggtcatcc atcagtgaaa ctggggtatt
     6841 catgaaaatg ttcctccgga tgttgttaat ctcaaccggc gttggtgatg aaaacaacgc
     6901 accaccttca ccggcataac aaccggggag aaacgtaaaa aataatcggg aatgtgaccg
     6961 tcaggagata aacgggggga ttgcggtggc tgagccgtac cggtatgtcg ggtgattgtt
     7021 gcgaaacgat gcttccgcac gcgcacggga ggactttcgt gagacaaggg gaaaactggc
     7081 gttctgataa ttgcagagtt gtttttgaag gcggttgtgg tgcatcagcc tgtgacctgt
     7141 gggcaggcga caggtatcac tggtgggata agactgtctg attggtatta taaatatata
     7201 agagagctca aacacatgag ttcatatatg tgaaataaat ctgtgagtgt gatcagcaga
     7261 ccagtctacc cagtagcaac agattgcgtt gtactcacag ggttatccct gataactggc
     7321 gtataacctg cgtacataaa acgtacctgc aatgcacctg aacagagtgc attaagcatc
     7381 cgcctgagcg gtaaacgtat ccgatctggt gagtgacagt ctgagagcat tctcagtaat
     7441 cgcccccttg tatcctggaa attggttaag agcgattaga tttggcaagg tgtatgcagc
     7501 cagcagctta acacctcagc taactggctg gttaccttca ggttgtcgca atggcatcgg
     7561 atatccggtg tcggataact aaacgtagcg ttaaggcgat attgctgaga gaccggttaa
     7621 gtggtggcac gggagtaatg agtttgggga gggtaccaga atgccagtca ggtgcggtat
     7681 gctggcagta tcacgttgat tgcagcataa agtaacgaga gacgaatgga agttaaaacc
     7741 agagcagttc cgtcgttgtt catcccggga gacgctggaa aaagtgagtt cccacacacg
     7801 ttataaactt acccctgcgg agctggaagc ctttaactct gcggtcgatc accggctggc
     7861 agaactgatc atgaacaaac tttacgatcg cgttccggct tctgtctgga aatatgtcat
     7921 ctgaacgttc ggtaatcaca gcggtattgc gccaggtgtt atctgctctg tgattctttt
     7981 gctcttctct ggttgctgca aagctgaata tgttcccgtt gttggaagcc gaggtaatac
     8041 cgggaacgtt aagcggtata gcgacaacaa agaattcgaa atcaaaaggg aatggtgtac
     8101 ggaggtgcgg gcggtaatgg gccgattttc gggcattacc gacctctgct gaggttttct
     8161 cgtatttacc ggggggccgg tgcgttactg tctggcacaa caaacgcagc gggtcgctct
     8221 gctccgccga ttcctgataa tccctcccca gtaacctacg gcaacagtac aaatacactg
     8281 acgcctgaaa cggtaaccag aatcagcacc agcagtaacg tcagtagaca gaacagacga
     8341 tcggtcttcc ggtaataacc ggaaggatcg acagctccac aatccgtaac cagtttttac
     8401 attaaaaaaa caacgccggt aattcaatcg tcagtccgga gacgaccgtt cgggttatca
     8461 cagagtgcat gagacattgc ctgctggaga taacttcagg ggctcttcgc attttttaca
     8521 gcgtggttat tccgtatgaa ccatacggag atttaaccat gacctacaaa tacaacccct
     8581 tctggcaaca acgtatccgt gagacggtgc tgcgcgcact ggatgttcat ccccgcctga
     8641 cggcacttcg ggttgatctg cgtctcccgg atgtaccggc agcaacggac gcagctgtga
     8701 tatcccgctt catcaatgcc ctgaaagcac gaatcgacgc ttaccagaag cgtaagcgcc
     8761 gtgaaggtaa acgcgtacat cccacgatcc tgcattacgc ctgggcccgg gaattcgggg
     8821 agcttaaagg caaaaaacac tatcacctcc tgctgctggt caaccgggat acctggtgtc
     8881 gtgccggcga ttaccgcgct ccgggatcac tggccgggat gattaaacag gcctggtgca
     8941 gtgccctggg ggtggatgcc ggatgctatg ccacgctggc gaattttccg gcctggccgg
     9001 tggtgtggct ggaacgcggt gatgacaccg gttttcagca ggtgctggag cgtgctgact
     9061 atctggcgaa ggagagtacc aaagcttacg gcaccggtga gcgcaacttt ggctgcagtc
     9121 gtggctgagc ccgacagacg acttatctga ccttagtgtg atgatgcgtt cacagcgacc
     9181 acactgattt tcccttctga gactgaactg aaaaccgcta ttcacacctg accacgctgc
     9241 ccgtaccggg acggctgttg cctgcgtgtc gtctggcggc tgaggaatat gactatgtac
     9301 gcaaaatcct ttctcgctct tgatggcaac gggcgtctga ccggtgcccg tactgcacag
     9361 actgcgccat acgatcgtta tacctgccat ctttgcggca gtgctctcag ataccatccg
     9421 caatacgaca ctgagcgtcc ctggtttgaa cacaccgacg acgggctgac tgaacacgct
     9481 cagcagtgtc cttacgtcag gcctgatcgc agggaagtgc aactggttaa acgtttacgg
     9541 caattcgtac cggatgcctt acccgtagta cgtaaagcca gctggtactg cagacaatgt
     9601 caccacgatt attatgggga gcggtactgc acacactgcc agaccgggcg cttcagtgaa
     9661 gaggggggag cagaatgaac ggtataaaaa cggggctggg gatcacgccg ggtgagcaca
     9721 ttatcagtgc cgactctgcg ctcagcagaa atatccggca ttgtttctgc ctttcctgtc
     9781 atggccggtt gattctgcag acagatgctc agggtgcgtg gtttgagcat gatttacatg
     9841 ccctgagtga gcaacagaag gcggactgtg tggtgctgaa tccggaaaag tcccaccctt
     9901 acattgagga tatggcgatg tttttatcgc cattaccggt cgtgctggag tggcattgtg
     9961 tgatgtgcgg gaggcttttt cccgggaaga agttctgtga ggcctgtggt acggggattt
    10021 attgcagagc ggtatgtacg aagtctgttt acagctacca gccagacttg tttgaggact
    10081 gcggaagtgc gagtacttaa tccgaaatcc ccgctctgcg cagggcgttc agaggtattg
    10141 ccaacctgta ttttatccgg tgtttcgcac ccaccccgtt cgtgacacaa aggcgcgaca
    10201 ccggatatcc gcagtcctgc ttacctgaat aaaacccggg gtattgattg gtagtgtggt
    10261 gcggaatcgt accgtgatct cgcattcgcc gaattcgcac tgtggtgtca atcaaccaca
    10321 cgggggcgtc gtctcagcct ttgcctgcct caggagggtg aaacctgggg ggcattgatt
    10381 aatatcctgc ccgatctgat gaccggtact tcagacctgc taccgatacg ccggaatata
    10441 acggtcagag aggcatattt cgatgcccct gttctggagc agttgatgat ggatgccata
    10501 ggcaatgtaa gcgctttgta tcaccggacg aagcacgcag taccagaagc cccccggggt
    10561 tcattggtga cctgacagcg aagatagatt caaagagcgt tcagtgattt ttcctgcgct
    10621 cgtcgtggtg tacgcaatca ggaatcccgt tatctcattg gcatacttgc gataccacac
    10681 cagcaggacg acgctgaaat cacgccgaca ttctcctgta tggcatcaat ctcacagggg
    10741 acattctgag catgttgacc tcaatcacag atcacgacag cgtgctcatt ttactgtctt
    10801 atatcattgg tgctgacgca ccggcccgac aaagggtttt acacgctgat tcagtgaaga
    10861 ccatttctct aaacccatca aactggggtg aagcaaccgc aagtacaaaa gtgaagtgcc
    10921 acagtagata cagcaacgaa tcatggaggt cagaagtatg aaacgtgttg tgataccggt
    10981 acgctggcaa tgtgcaaaat gccagcgctg gtattgtgga acccggccct gcccttggtg
    11041 ctggcgatat tcccgttcat cttcctgctg ataccttccg ttcagcagcg ggttgttatc
    11101 ccttcctgac tgattcgtca ttccattctt attgattaga actggcatta caccggtgcc
    11161 ggcgtgcttt cctgcgtgtc tgcgccggtt cagtaaatta gaaaagaaac atttgtcagc
    11221 gccagtgata taaggcgtaa cggctaacaa tcttggcgtt aatacattcc gagcaaataa
    11281 ccgaagcgtt acttttagtg acggggttat ccaatacttt tctagtcagt gtttagtatt
    11341 tatgcaagcg atataagtag tcaccgctta gactattgac attcatgatg cctgttaatt
    11401 caaatttcag gtatgatcag tcatttattg ttgtgatact gattccgcta gtttagcata
    11461 actgtcctgc aaatcattgt tcaaaaaaga aagctcctac aaactgtatc ctgtatctct
    11521 accagaagat aatatacttg gttatttttg tgcataagct cacataaaaa taatattaat
    11581 tatgttttct tagagataga aaaataaaca ggtaagcaga agacggactt ttttaatgcg
    11641 aaaacgtatt ctttttattg gcccaccgct gtacggtttg ttatacccat tgatttctct
    11701 ggctcaggcc tttcgtgtaa ttggacatga tgtagtaatt agtagcgctg gcaaattcgc
    11761 gaataaagca gcagaagctg gactggttgt ttttgatgca gttccaggtt tagattcaga
    11821 ggctggatat cgccatcagg aagagttgag gaaaaaaagt aatattattg gtcatttctc
    11881 tttttttagc gatgaaatgg cagataacct catcgatttt gcaggaaaat ggaggccaga
    11941 tttaatagtc tatccccctc ttggtccggc aggcccattg gttgctgcta aatatagaat
    12001 tccttcagtg atgctggctg ttggattcgc gcatacatct gcccatattc agatgttaaa
    12061 ccgttcttta agcaatgctt acaggcggca tggagtcagc ggtccactat gtgatttagc
    12121 atggattgat gttgctcccc caagtatgag cattcttaaa aatgctgggg aaccggttat
    12181 ctcaatgaga tatattcctt ataacggagg tgctgtaaag gaaacatggt gggacaggga
    12241 ttctgatcgg aaacgtttac ttatcagcct tggcactgta aaaccaatgg ttgatggtct
    12301 ggagctgatt tcatgggtta tggattctgc aaatgaagtt gatgctgata tcattttgca
    12361 acttgcaata aatgctcgta ctggtttacg aaaactacca tcaaatgtac gtctggttga
    12421 ctggatacct atgggtgtat tccttaatgg agctgatgga tttattcatc acggtggcgc
    12481 aggtaatacc ctgacagcgc tgtatagtgg aataccacag attgtgtttg gcgaaggtgc
    12541 agatcgctct gttaatgcag aaattgttgc gaagcgtggg tgtgggatta ttccggacaa
    12601 gcatggactg accagtgatt tggtaaatcg cctgctttat gatgattcac tacgcttctg
    12661 ttcagatcag gtagccgctg aaatggctga acaacccagt cctgcagaga ttgcagaggt
    12721 tttgatgaga aaattaaaaa acaacgggaa acaattgtag cgtattgctg aaattttcaa
    12781 cgagaaatgt tatgagtaat ataatgtcct tcatcacata aatgaaggac atcactaaat
    12841 aaatgagata gtctcccata ataatattgc agaaagagtt atcggacgat aaaacgatat
    12901 tttacacaga gttattattt gataccttcc tgcaattata atttgctaat tcatcaataa
    12961 ttgctccgcg ccaccaagca taatcgtgac caccggtata gacagtgagg ttactttcca
    13021 aaccagctgc gattaacgaa tgatgcaatc gctgaacatg tgagacggtt gtaccttcta
    13081 aagaaccgac tccaagtcgg atatttacat ctttcggagg cgctgaaagt atctgctcac
    13141 ttacccagga ggtattattc tcagtaaaca agatcgggct gccctggtca gggttccacc
    13201 acattgaagg tgagtgacta atgattgtac caaatgttgt cgacgcatat atagctgcca
    13261 tcagtgctgt cactccaccg aggctctgcc cggccagtac agtattagag cgaccagccc
    13321 atacgatatc cggatagtct ctgtataact ggggtatcag attttccgct atatcaaaga
    13381 tgagttcttt attgcctccc agtatattca tacgatcaga ttcattaata ttgtctatcc
    13441 ccataattgc cattggcgtg atacgaccag tggtgatggc catatctaat gcccgggtaa
    13501 tatctatccg gtcaaaccat gtttcagcat caggtaacac aagtaatcca agaggtactg
    13561 atgttggatt caggggaaaa tataaacgaa tccggcgttg atatcctgca acaaaggaat
    13621 gtaatgttga aagaatacct gtacatttat gggaagtatc atcccattcc ttttgttcag
    13681 gagccatatc aagagaaagg actgattctc cgaatcctcg tatatttatt tctgctgttt
    13741 tgttaaatgg atcagattta ccgggtaatg gagagaagcg acttcctagt tgaaatatgt
    13801 ctttttgtgt catatctgtg ggaatttcta taaatgagta tgagccccga tatgaagctg
    13861 gtaacaccag tgtcagcatc cagatatctg tcgaagggat atgagtcatt agtccttttt
    13921 tgacgtcttt tttatctgta acacgattca ggcgaagata tacgccttgc agtgctttct
    13981 ctgagcgcca cagaaatgta acctcccgat tttctgtagt ggtgcttttt gtgatcactg
    14041 gaagtgttgc tgactcaaga tagtgccaga aattagcaat atctgttgtt cttattgtga
    14101 caggatgtgt catttttttt aactgtacac aaatatcttc gggtgtctgg aaaagatagt
    14161 ttttcatagg tatca
//
LOCUS       DAZGVQ010000128        14102 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000128 DAZGVQ010000000
VERSION     DAZGVQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.129"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            231..530
                     /gene="yoaC"
                     /locus_tag="ACHLAG_003804"
     CDS             231..530
                     /gene="yoaC"
                     /locus_tag="ACHLAG_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFP0656949.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERTVIKNPEYFSTYMQEELKALV"
     gene            complement(650..829)
                     /gene="yoaH"
                     /locus_tag="ACHLAG_003805"
     CDS             complement(650..829)
                     /gene="yoaH"
                     /locus_tag="ACHLAG_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFP0656950.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            903..2264
                     /gene="pabB"
                     /locus_tag="ACHLAG_003806"
     CDS             903..2264
                     /gene="pabB"
                     /locus_tag="ACHLAG_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFP0656951.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2268..2846
                     /gene="nudL"
                     /locus_tag="ACHLAG_003807"
     CDS             2268..2846
                     /gene="nudL"
                     /locus_tag="ACHLAG_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFP0656952.1"
                     /translation="MEYRSLTLDYFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3030..4394
                     /gene="sdaA"
                     /locus_tag="ACHLAG_003808"
     CDS             3030..4394
                     /gene="sdaA"
                     /locus_tag="ACHLAG_003808"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP0656953.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMCFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4525..6123
                     /gene="pdeD"
                     /locus_tag="ACHLAG_003809"
     CDS             4525..6123
                     /gene="pdeD"
                     /locus_tag="ACHLAG_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0656954.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6127..7683)
                     /gene="yoaE"
                     /locus_tag="ACHLAG_003810"
     CDS             complement(6127..7683)
                     /gene="yoaE"
                     /locus_tag="ACHLAG_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFP0656955.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7671..7829)
                     /gene="yoaL"
                     /locus_tag="ACHLAG_003811"
     CDS             complement(7671..7829)
                     /gene="yoaL"
                     /locus_tag="ACHLAG_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFP0656956.1"
                     /translation="MDRHRRHFSIRPFNACLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8146..9117
                     /gene="manX"
                     /locus_tag="ACHLAG_003812"
     CDS             8146..9117
                     /gene="manX"
                     /locus_tag="ACHLAG_003812"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFP0656957.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9180..9980
                     /gene="manY"
                     /locus_tag="ACHLAG_003813"
     CDS             9180..9980
                     /gene="manY"
                     /locus_tag="ACHLAG_003813"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFP0656958.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9993..10844
                     /gene="manZ"
                     /locus_tag="ACHLAG_003814"
     CDS             9993..10844
                     /gene="manZ"
                     /locus_tag="ACHLAG_003814"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFP0656959.1"
                     /translation="MVDTTQTTTGKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10899..11357
                     /gene="yobD"
                     /locus_tag="ACHLAG_003815"
     CDS             10899..11357
                     /gene="yobD"
                     /locus_tag="ACHLAG_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFP0656960.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            11786..12352
                     /gene="mntP"
                     /locus_tag="ACHLAG_003816"
     CDS             11786..12352
                     /gene="mntP"
                     /locus_tag="ACHLAG_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFP0656961.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(12349..13158)
                     /gene="rlmA"
                     /locus_tag="ACHLAG_003817"
     CDS             complement(12349..13158)
                     /gene="rlmA"
                     /locus_tag="ACHLAG_003817"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFP0656962.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(13324..13533)
                     /gene="cspE"
                     /locus_tag="ACHLAG_003818"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(13324..13533)
                     /gene="cspE"
                     /locus_tag="ACHLAG_003818"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP0656963.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(13546..13689)
                     /gene="yobF"
                     /locus_tag="ACHLAG_003819"
     CDS             complement(13546..13689)
                     /gene="yobF"
                     /locus_tag="ACHLAG_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFP0656964.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
BASE COUNT         3501 a         3508 c         3491 g         3602 t
ORIGIN      
        1 actagcaact ctggttttct ttcttagctg aataatctgg ttatagcgtg aggactgacc
       61 gttaaatttc tccatcttac tggataacac accatgagct gctaccttta aaacacctga
      121 atgtcaacag gttaactcgt gccatatcgt tataatccct ttgatattag atgcaaatta
      181 aggtcatata tagtcttatc gataacatgg ttaattttaa ggataagaat atgcctgctg
      241 taatagataa agccctggat ttcattggtg ccatggatgt atcagcgcca acaccaagtt
      301 cgatgaatga aagcacagcg aagggaatct ttaaatattt aaaagaactg ggagtacccg
      361 cgagtgccgc tgatattacg gcgcgagctg accaggaagg ctggaaccct gggttcacgg
      421 aaaaaatggt tggatgggca aaaaaaatgg agtctggtga acgcactgta attaaaaatc
      481 ctgaatactt ttcaacatat atgcaggaag aactaaaagc actggtctga gcttaattta
      541 tatcagcata aatgggtcag gacgctttta atcacatata aaaagcgtcc tttttctcac
      601 aatcaaataa tcatcggatt ctataacgaa atcgatacgg attagtgtgt tactcgtctt
      661 catcctcaaa acgcgccacg attcgctcgc cggagtggtt ggcacgcagc tcttctgcca
      721 ccagcgcgat tgcctggccg ctgctcatcc cttgtgccat cagttcctgg atccgctcga
      781 cagctttttg ctgctgttca tgggtgagtg aaggtaaacc tgcaaacatt gttaactcct
      841 gctaaattgt tggcgctaat tatttcatgc tacccggcac atagccagta gagtcaggac
      901 tgatgaagac gttatctccc gctgtgatta ctttaccctg gcgtcaggac gccgctgaat
      961 tttatttctc ccgcttaagc cacctgccgt gggcgatgct tttacactcc ggctatgccg
     1021 atcatccgta tagccgcttt gatattgtgg tcgccgatcc gatttgcact ttaaccactt
     1081 tcggtaaaga aaccgttgtt agtgaaagcg aaaaacgcac aacgaccact gatgacccgc
     1141 tacaggtgct ccagcaggtg ctggatcgcg cagacattcg cccaacgcat aacgaagatt
     1201 tgccatttca gggcggcgca ctggggttgt ttggctacga tctgggccgc cgttttgagt
     1261 cactgccaga aattgcggaa caagatatcg ttctgccgga tatggcagtg ggtatctacg
     1321 attgggcgct cattgtcgac caccagcgtc atacagtttc tttgctgagt cataatgatg
     1381 tcaatgcccg tcgggcctgg ctggaaagcc agcaattctc gccgcaggaa gatttcacgc
     1441 tcacttccga ctggcaatcc aatatgaccc gcgagcagta cggcgaaaaa tttcgccagg
     1501 tacaggaata tctgcacagc ggtgattgct atcaggtgaa tctcgcccag cgttttcatg
     1561 cgacctattc tggcgatgaa tggcaggcat tccttcagct taatcaggcc aaccgcgcgc
     1621 catttagcgc ttttttacgt cttgaacagg gtgcaatttt aagcctttcg ccagagcggt
     1681 ttattctttg tgataatagt gaaatccaga cccgcccgat taaaggcacg ctaccacgcc
     1741 tgcccgatcc tcaggaagat agcaaacaag cagaaaaact ggcgaactca gcgaaagatc
     1801 gtgccgaaaa tctgatgatt gtcgatttaa tgcgtaatga tatcggtcgt gttgccgtag
     1861 ccggttcggt aaaagtacca gagctgttcg tggtggaacc cttccctgcc gtgcatcatc
     1921 tggtcagcac tataacggcg cgactaccag aacagttaca cgccagcgat ctgctgcgcg
     1981 cagcttttcc tggtggctca ataaccgggg ctccgaaagt acgggctatg gaaattatcg
     2041 acgaactgga accgcagcga cgtaatgcct ggtgcggcag cattggctat ttgagctttt
     2101 gcggcaacat ggataccagc attactatcc gcacgctgac tgccattaac ggacaaatat
     2161 actgctctgc gggcggtgga attgtcgccg atagccagga agaagcggaa tatcaggaaa
     2221 cttttgataa agttaataag atattacgcc aactggagaa gtaagacgtg gaataccgta
     2281 gcctgacgct tgattatttt ttatctcgct ttcaactttt gcgcccgcaa attaaccggg
     2341 aaaccctaaa tcatcgtcag gctgccgtgt taatccccat cgtccgtcga ccgcaaccgg
     2401 ggttgttgct gactcagcgt tcaattcatc tgcgtaaaca cgctggacaa gtggcattcc
     2461 ctggaggtgc agtcgatgac acggacgcat cagttatcgc cgccgcgctg cgcgaagctg
     2521 aagaagaggt cgctataccg ccttccgccg ttgaagttat cggcgtgctg ccgcccgtcg
     2581 atagcgtaac tggctatcag gtaaccccag tggtcggcat tatcccgccc gatctgccgt
     2641 atcgcgccag tgaagatgaa gtctcggcgg tgtttgaaat gccgctcgcc caggcattac
     2701 atctgggtcg ttatcaccct ttagatatct accgccgtgg cgattcacat cgggtatggc
     2761 tgtcctggta cgaacagtat tttgtatggg gaatgaccgc aggcataatt cgtgagctgg
     2821 cgctgcaaat tggtgtgaaa ccctgactat acttatcttt acatctacaa aacactactt
     2881 gagacaatca tcgcaatatt agttaaatcg cggtttttga ttagtttaat tcatgtgaat
     2941 agttaagcca gtcgccgcgt tccctcttac actatgcgct gttattagtt cgttactgga
     3001 agtccagtca ccttgtcagg agtattatcg tgattagtct attcgacatg tttaaggtgg
     3061 ggattggtcc ctcatcttcc cataccgtag ggcctatgaa ggcgggtaaa cagttcgtcg
     3121 atgatctggt cgaaaaaggc ttactggata gcgttactcg cgttgccgtg gacgtttatg
     3181 gttcactgtc gctgacgggt aaaggccacc acaccgatat cgccattatt atgggtcttg
     3241 caggtaacga acctgccacc gtggatatcg acagtattcc cggttttatt cgcgacgtag
     3301 aagagcgcga acgtctgctg ctggcacagg gacggcatga agtggatttc ccgcgcgaca
     3361 acgggatgtg ttttcataac ggcaacctgc cgctgcatga aaacggtatg caaatccacg
     3421 cctataacgg cgatgaagtc gtctacagca aaacttatta ttccatcggc ggcggtttta
     3481 tcgtcgatga agaacacttt ggtcaggatg ctgccaacga agtgagcgtg ccgtatccgt
     3541 tcaaatctgc caccgaactg ctcgcgtact gtaatgaaac cggctattcg ctgtctggtc
     3601 tcgctatgca gaacgaactg gcgctgcaca gcaagaaaga gatcgacgag tatttcgcgc
     3661 atgtctggca aaccatgcag gcatgtatcg atcgcgggat gaacaccgaa ggtgtactgc
     3721 caggcccgct gcgcgtgcca cgtcgtgcgt ctgccctgcg ccggatgctg gtttccagcg
     3781 ataaactgtc taacgatccg atgaatgtca ttgactgggt aaacatgttt gcgctggcag
     3841 ttaacgaaga aaacgccgcc ggtggtcgtg tggtaactgc gccaaccaac ggtgcctgcg
     3901 gtatcgttcc ggcagtgctg gcttactatg accactttat tgaatcggtc agcccggaca
     3961 tctatacccg ttactttatg gcagcgggcg cgattggtgc attgtataaa atgaacgcct
     4021 ctatttccgg tgcggaagtt ggttgccagg gcgaagtggg tgttgcctgt tcaatggctg
     4081 ctgcgggtct tgcagaactg ctgggcggta gcccggaaca ggtttgcgtg gcggcggaaa
     4141 ttggcatgga acacaacctt ggtttaacct gcgacccggt tgcagggcag gttcaggtgc
     4201 cgtgcattga gcgtaatgcc attgcctctg tgaaggcgat taacgccgca cggatggctc
     4261 tgcgccgcac cagtgcaccg cgcgtctcgc tggataaggt catcgaaacg atgtacgaaa
     4321 ccggtaagga catgaacgcc aaataccgcg aaacctcacg cggtggtctg gcaatcaaag
     4381 tccagtgtga ctaatacttc ttactcgccc atctgcaacg gatgggcgaa tttatacccg
     4441 ctttctcgtc tgctgtaata ttccccacta cacttccact gttgcgtcag gcgtttgtcg
     4501 ccatacgctt acagggtggc ccgcatgcaa aaagcacaac ggatcattaa aacctatcgc
     4561 cgtaatcgaa tgattgtttg tacgatttgc gcactcgtta cgctcgcttc gaccctgagc
     4621 gtgcgattta tttcacagcg taacttaaat caacaacggg tagtacaatt cgccaatcac
     4681 gctgtagagg aattagataa agtactgctt cccctacagg caggtagcga agtcttgctt
     4741 ccgctgattg gtctgccctg ctctgtcgcc catttgccat tacgtaaaca ggcggcaaaa
     4801 ctccaaactg tgcgatccat tggcctggtg caagacggca cactttattg ctccagcatt
     4861 tttggttatc gcaatgtgcc cgtcgtggac attctggctg aacttcctgc accgcaacca
     4921 cttttacgcc tgacgatcga ccgtgccctg attaaaggca gtccggtttt gattcaatgg
     4981 acgcctgcag cgggcagtag caatgctggg gtcatggaga tgattaacat cgacttactg
     5041 acggcaatgc tccttgagcc acaactgccg caaatcagta gcgccagcct gacggtggac
     5101 aaacggcatt tgctctatgg taatgggctg gtagattccc ttccgcaacc tgaagacaat
     5161 gaaaactatc aggtttcttc gcaacgcttt ccttttacca ttaacgttaa tggtccgggg
     5221 gctacggcgc tggcatggca ctatcttcca acacaattac cgctggcggt gctgctaagt
     5281 ttactggtgg gctacatcgc ctggctggcg accgcttacc ggatgagctt ttcccgcgaa
     5341 atcaatctgg gcctggcgca acatgagttc gaattgttct gtcagccttt gcttaatgcg
     5401 cgcagccagc aatgtattgg tgtagagatt ttgctgcgct ggaacaatcc gcgtcagggc
     5461 tggatttcac cggatgtgtt tattcctatc gcggaagaac atcatttaat tgtgccactg
     5521 acccgctatg tgatggcaga aaccattcgt cagcgccatg ttttcccgat gagtagtcag
     5581 tttcatgttg gcattaacgt cgcacccagc cattttcgcc gtggtgtgct gataaaagat
     5641 ctcaatcagt actggtttag cgctcacccg attcagcaac tgatcctcga aatcaccgaa
     5701 cgcgatgcct tactggatgt tgattatcgg attgcccgcg agctacatcg taaaaacgtc
     5761 aaactggcga ttgatgactt cggcaccggc aacagttcat tttcctggct tgaaacatta
     5821 cgtcctgacg tgctgaaaat tgataagtca tttaccgcag ctataggttc tgacgcggtt
     5881 aactcgacgg tgaccgatat catcatcgcg ctggggcaaa gactgaatat tgaactggtg
     5941 gcggagggcg tggaaacgca agaacaggcg aagtatttgc gccgtcatgg cgtgcatatt
     6001 ttgcaagggt atttgtacgc acagccgatg ccgctacgtg attttcccaa atggctggcg
     6061 ggcagccaac cgccgcccgc ccggcataat ggacatatca cgcccattat gccgttacgt
     6121 taacgcttac tcatcttcat cgtgcgccgg ttgctcttta acaatgcgaa ccagatcaac
     6181 acgataatca ttggcttcaa tgatggtgat atgcagtggc cctacatcaa tcacatcgcc
     6241 cacacgggga atgtgaccat ttgccgagat caccaggccc gcgaccgtcg cgatatcatc
     6301 gtcatcggca aggtgctcaa catcaagcgc ctgctgcaag gcatgcaaat ctgtaccgcc
     6361 ttttaccagc cagccgtcac catcggtaat gatttccggc gtttcgtcag cgtccgggaa
     6421 ttcacccgca atggcttcca gcacatccag cggcgtgacc aagccttgta ccacaccaaa
     6481 ctcgttggtc acgataacaa agctcccgcg agcgcgacgc agcacgccca gcaggttgat
     6541 cggatcgagg gtttccggga cgataatcgc cggagacgcc gaagcaatcg ccgccacatc
     6601 aacgccctct tccagcgcca ccagcagttc tttagcacgt acgattccga tgatttcatc
     6661 cagttcaccg cgacataccg ggaacaggct gtgcggtgag gagagcagct gctcgcggat
     6721 ttcatcgacc ccgagattag cgtcaaccca gcttatttca ccgcgcggcg tcatgatccc
     6781 gcgcagagaa cgcgacgcca gcgtcagtac gccgttaatc atgtaacgtt cttcttcggc
     6841 aaatgcacct tccgggatcg gcatcggcat cgggttatcg gcatcgtgct gaacattggc
     6901 ctgacgtttc ccgcccatca aacgcaggat ggcatcggca gtacgcgctc gcagcggcaa
     6961 agtcgactgg tgacgaataa agttgcgacg cgcaatctgg ttaaacactt cgatgatgat
     7021 cgagaagcca atcgcggcat acaggtaacc tttcggaatg tggaaaccga aaccttctgc
     7081 caccagactc agaccaatca ttaacaggaa gctcagacag agcaccacca ccgtggggtg
     7141 ctggttaacg aatcgcgtca gcggtttgga tgccagcaac ataaccgcca tcgcaatcac
     7201 taccgccgcc atcatcaccg gcagatggtt aaccatccct actgcagtaa ttaccgcatc
     7261 caacgagaag acggcgtcaa ggatgacaat ctgtgtgacg accacccaga aactggcgta
     7321 gcctttaccg tggccggaat catgatcgcg gttttccagc cgttcatgca gttcggttgt
     7381 tgctttgaac agcaagaata tccccccgaa caacataatc aggtcgcgtc cggagaagga
     7441 gaaatccatg acggtaaata gcggtttggt cagcgtgacc atccatgaaa tcagcgacag
     7501 cagccccaga cgcataatca gcgccagtga taaccccagc aaacgcgctt tatcgcgttg
     7561 ttttggcggc agtttgtcag caagaatggc gatgaagacc aggttatcga tacccagcac
     7621 aatttcgaga acaacaagcg tgagtagccc cgcccaaatt gaggggtcca ttaagaattc
     7681 catgacaagc tcctgcttaa ggaatagcta ttcgacgcca gaaataatgc aggcgtaacg
     7741 acaaaatgca aacgaaaggt gcggcataga gagccagaaa ggcaggcgtt aaaaggcctg
     7801 atgctgaaat gacgtcggtg acgatccata ctgcgggcta ctgccctata ctccatggtt
     7861 gttaaacggg agttaaacat atcagagacg cctctgattt ggcaaagatt taccttcctt
     7921 tgcaaacgaa tgtgacaagg atattttacc tttcgaaatt tctgctaatc gaaagttaaa
     7981 ttacggatct tcatcacata aaataatttt ttcgatatct aaaataaatc gcgaaacgca
     8041 ggggtttttg gttgtagccc ttatctgaat cgattcgatt gtggacgacg attcaaaaat
     8101 acatctggca cgttgaggtg ttaacgataa taaaggaggt agcaagtgac cattgctatt
     8161 gttataggca cacatggttg ggctgcagag cagttgctta aaacggcaga aatgctgtta
     8221 ggcgagcagg aaaacgtcgg ctggatcgat ttcgttccag gtgaaaatgc cgaaacgctg
     8281 attgaaaagt acaacgctca gttggcaaaa ctcgacacca ctaaaggcgt gctgtttctc
     8341 gttgatacat ggggaggcag cccgttcaat gctgccagcc gcattgtcgt cgacaaagag
     8401 cattatgaag tcattgcagg cgttaacatt ccaatgctcg tggaaacgtt aatggcccgt
     8461 gatgatgacc caagctttga tgaactggtg gcactggcag tagaaacagg ccgtgaaggc
     8521 gtgaaagctc tgaaagccaa accggttgaa aaagccgcgc cagcacccgc tgccgcagca
     8581 ccaaaagcgg ctccaactcc ggcaaaacca atggggccaa acgactacat ggttattggc
     8641 cttgcgcgta tcgacgaccg tctgattcac ggtcaggtcg ccacccgctg gaccaaagaa
     8701 accaatgtct cccgtattat tgttgttagt gatgaagtgg ctgcggatac cgttcgtaag
     8761 acactgctca cccaggttgc acctccgggc gtaacagcac acgtagttga tgttgccaaa
     8821 atgattcgcg tctacaacaa cccgaaatat gctggcgaac gcgtaatgct gttatttacc
     8881 aacccaacag atgtagagcg tctcgttgaa ggcggcgtga aaatcacctc tgttaacgtc
     8941 ggtggtatgg cattccgtca gggtaaaacc caggtgaata acgcggtttc ggttgatgaa
     9001 aaagatatcg aggcgttcaa gaaactgaat gcgcgcggta ttgagctgga agtccgtaag
     9061 gtttccaccg atccgaaact gaaaatgatg gatctgatca gcaaaatcga taagtaacgt
     9121 attgtgttga ttatcactca gttttcacac ttaagtctta cgtaaacagg agaagtacaa
     9181 tggagattac cactcttcaa attgtgctgg tatttatcgt agcctgtatc gcaggtatgg
     9241 gatcaatcct cgatgaattt cagtttcacc gtccgctaat cgcgtgtacc ctggtgggta
     9301 tcgttcttgg ggatatgaaa accggtatta ttatcggtgg tacgctggaa atgatcgcgc
     9361 tgggctggat gaacatcggt gctgcagttg cgcctgacgc cgctctggct tctatcattt
     9421 ctaccattct ggttatcgca ggtcatcaga gcattggtgc aggtatcgca ctggcaatcc
     9481 ctctggccgc tgcgggccag gtactgacca tcatcgttcg tactattacc gttgctttcc
     9541 agcacgctgc ggataaggct gctgataacg gcaacctgac agcgatttcc tggatccacg
     9601 tttcttctct gttcctgcaa gcaatgcgtg tggctattcc ggccgtcatc gttgcgctgt
     9661 ctgttggtac cagcgaagta cagaacatgc tgaatgcgat tccggaagtg gtgaccaatg
     9721 gtctgaatat cgccggtggc atgatcgtgg tggttggtta tgcgatggtt atcaacatga
     9781 tgcgtgctgg ctacctgatg ccgttcttct acctcggctt cgtaaccgca gcattcacca
     9841 actttaacct ggttgctctg ggtgtgattg gtactgttat ggcagtgctc tacatccaac
     9901 ttagcccgaa atacaaccgc gtagccggtg cgcctgctca ggcagctggt aacaacgatc
     9961 tcgataacga actagactaa caggtgagcg aaatggttga tacaactcaa actaccaccg
    10021 ggaaaaaact cactcaaagt gatattcgtg gcgtcttcct gcgttctaac ctcttccagg
    10081 gttcatggaa cttcgaacgt atgcaggcac tgggtttctg cttctctatg gtaccggcaa
    10141 ttcgtcgcct ctaccctgag aacaacgaag ctcgtaaaca agctattcgc cgtcacctgg
    10201 agttctttaa cacccagccg ttcgtggctg cgccgattct cggcgtaacc ctggcgctgg
    10261 aagaacagcg tgctaatggc gcagagatcg acgacggtgc tatcaacggt atcaaagtcg
    10321 gtttgatggg gccgctggct ggtgtaggcg acccgatctt ctggggaacc gtacgtccgg
    10381 tatttgcagc actgggtgcc ggtatcgcga tgagcggcag cctgttaggt ccgctgctgt
    10441 tcttcatcct gtttaacctg gtgcgtctgg caacccgtta ctacggcgta gcgtatggtt
    10501 actccaaagg tatcgatatc gttaaagata tgggtggtgg cttcctgcaa aaactgacgg
    10561 aaggggcgtc tatcctcggc ctgtttgtca tgggggcatt ggttaacaag tggacacatg
    10621 tcaacatccc gctggttgtc tctcgcatta ctgaccagac gggcaaagaa cacgttacta
    10681 ctgtccagac tattctggac cagttaatgc caggcctggt accactgctg ctgacctttg
    10741 cttgtatgtg gctactgcgc aaaaaagtta acccgctgtg gatcatcgtt ggcttcttcg
    10801 tcatcggtat cgctggttac gcttgcggcc tgctgggact gtaagactgt tgtacactac
    10861 cggggccttt tggccccatt tttttatctg gaggattaat gacaatcacg gacctggtac
    10921 tgattctttt catcgccgca ctcctggcct tcgcgatcta cgatcagttc atcatgcccc
    10981 gccgtaacgg ccccaccctg ctggcaattc ctttgctccg gcgtggtcgc atcgatagcg
    11041 ttatcttcgt cggattgatt gtcattctta tctataacaa cgtcacgaat catggtgcgt
    11101 taataacgac atggttatta agcgcactgg ctctgatggg tttttatata ttctggatcc
    11161 gcgttccgaa gatcatcttt aaacaaaaag gttttttctt cgccaatgtc tggattgaat
    11221 atagccgaat caaagcgatg aacttgtcgg aagatggcgt gttggtgatg caattagaac
    11281 agcgtcggct gttaatccgc gttcgaaata tcgacgatct ggaaaaaatt tataagcttc
    11341 tcgtttcaac tcaataagtt atgaatttag ccaaagctat gtttagtgta tttttaataa
    11401 tcagacatag cttaggctat attacctctt cccttatttg ttatttattt taacgtttca
    11461 ttgatatata aatctaaatg taaatcgtta tcaataaagc aatgaaataa tatattccaa
    11521 cagttgtttt atattctcaa aatatgttaa ggttgcgccc tcatttgggg agtagccgat
    11581 ttccagattc cggaaatgta cgtgtcaaca tactcgttgc aaaacgtggc acgtacggac
    11641 tgaatacttt cagtcaggcg agaccatatg cacatcaatc gctatgcctg catgaacgcg
    11701 aaaaccatcg cggcttgttg ttcatgtatg catgtttgct gggggcgatg atgtgtttta
    11761 tggatacccc ggtcaggacg ttgtcatgaa tatcactgct actgttcttc ttgcgtttgg
    11821 tatgtcgatg gatgcatttg ctgcatcaat cggtaaaggt gccaccctcc ataaaccgaa
    11881 attttctgaa gcattgcgaa ccggccttat ttttggtgcc gtcgaaaccc tgacgccgct
    11941 gatcggctgg ggaatgggca tgttagccag ccggtttgtc cttgaatgga atcactggat
    12001 tgcgtttgtg ctgctgatat tcctcggcgg gcgaatgatt attgagggtt ttcgtggcgc
    12061 agatgatgaa gatgaagagc cgcgccgtcg gcacggtttc tggctactgg taaccaccgc
    12121 gattgccacc agcctggatg ccatggctgt gggtgttggt cttgctttcc tgcaggtcaa
    12181 cattatcgcg accgcattgg ccattggttg tgcaaccttg attatgtcga cattagggat
    12241 gatggttggt cgctttatcg gctcaattat tgggaaaaaa gcggaaattc tcggcgggct
    12301 ggtgctgatc ggcatcggcg tccagatcct ctggacgcac ttccacggtt aataagaacg
    12361 ctgccagagg tgaatattaa agtccgtctg gcagtcgaac acttcttttg ctgccagtgt
    12421 ttgccagact tctggcttcg cacgccaggc aaacggcgtc atctgcaata atgcgacggc
    12481 ttcatcaccg cgaagacgca tcggataaca caactcatca ctctgctgta atgtaaaacc
    12541 ttccagttgt tctgcatgag gtgcatgaag atgtacttca ttgtaaatca gccccttcag
    12601 ctccatcaaa tgtcgcggtc ccggcgtggc agtaatgacc cagccgccgg gcttcactac
    12661 tcgtgctaat tcttctgctt tacacggcgc gtaaatacgt attatggcgt ccatactggt
    12721 atcggaaaac ggcaaacggt ggctggaagc gacacaaaga gtgacctgcg gatagcgttt
    12781 cgccgccgct tttatcgcta ccttcgagac atccaggcca aacgtggtga tttcgggcaa
    12841 cacatcggca aatgcgtgtg tgtaataccc ttcaccacag ccaatatcca gcaccgccgt
    12901 ggccttttca tcaagccgtt cccgcagttg ggcgacaatt gcatcacgca gcggctgata
    12961 atgtccggca tctaagaatg cgcggcgtgc ttgtatcatt tccgcgctgt cgcccggatc
    13021 acgagaccgt ttatgctgaa cgggcagcag attgacatac ccttctttcg ccatatcaaa
    13081 ctgatgtcgc tggggacaga tatagctgtt tttttcacgc gaaagaggct gatggcaaag
    13141 tggacaggaa aacgacatga caactccggc aggatactaa aggccgcaag tgtaacgcga
    13201 atcacgcggc gggcaaatat ccgaacgcca ggagcgaaga taaagcatag taaaaagcct
    13261 cgcattcgac gaggctttat agcgagattg aagcgtattc acacttcaga tcagtggatt
    13321 cgatcagata gctgttacgt taacagctgc cggacctttc tggccgtcct gaatttcgaa
    13381 ctcaacgttc tgaccttcag ccagagtttt gaagccatta ccctggatag cggagaagtg
    13441 tacgaacaca tctttgctgc catcagccgg agtaatgaag ccaaaacctt tagactcgtt
    13501 gaaccactta acctgacctt taatctttgc catttgaaaa attccttaga ttgttttctt
    13561 cgcccgcagg cataacatag ataaaactga cacattactg catgaggcac caatataagg
    13621 ctcggcagag aagcggtatt caacgtcaac gtgtttactc aggacttctt tactgaaaat
    13681 gccacacata aacagaactg tacctcgttt aacccgaaat ctgttatcac ataccacgtt
    13741 aattatggca agccattttt aaacatgtct cgatcagacg cacaaatctc gatacgtctt
    13801 ccactttttt tgcacactta tgcaacggaa tacgcgccga tatatcattg tgcttaacct
    13861 tgccagttca ggcagatact taaactggcg tattttctaa catagttcaa taagttcagt
    13921 tgttccaggt cagtgggaga agttattaca tagtgcgtgc aatatcacat tttttgctat
    13981 gcaatgaata aaaagttata tcactttttc tcataaaaca gtcagttaac ggctatcaat
    14041 taccctaaag agaagtcaat ccccaaaggg attgaaaatt taaaataaga aaaattgatg
    14101 aa
//
LOCUS       DAZGVQ010000129        13874 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000129 DAZGVQ010000000
VERSION     DAZGVQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13874
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.130"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(24..335)
                     /locus_tag="ACHLAG_003820"
     CDS             complement(24..335)
                     /locus_tag="ACHLAG_003820"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP0656965.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            534..1232
                     /locus_tag="ACHLAG_003821"
     CDS             534..1232
                     /locus_tag="ACHLAG_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0656966.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(1277..2176)
                     /gene="eamA"
                     /locus_tag="ACHLAG_003822"
     CDS             complement(1277..2176)
                     /gene="eamA"
                     /locus_tag="ACHLAG_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFP0656967.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2370..3557
                     /gene="ydeE"
                     /locus_tag="ACHLAG_003823"
     CDS             2370..3557
                     /gene="ydeE"
                     /locus_tag="ACHLAG_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFP0656968.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPVNIRAL
                     MIAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFIILALVIIAAWVLMLKGIRAKPWGQP
                     ALC"
     gene            3684..3779
                     /gene="mgtS"
                     /locus_tag="ACHLAG_003824"
     CDS             3684..3779
                     /gene="mgtS"
                     /locus_tag="ACHLAG_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFP0656969.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            3779..3883
                     /gene="mgtT"
                     /locus_tag="ACHLAG_003825"
     CDS             3779..3883
                     /gene="mgtT"
                     /locus_tag="ACHLAG_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFP0656970.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(3998..4888)
                     /gene="dgcZ"
                     /locus_tag="ACHLAG_003826"
     CDS             complement(3998..4888)
                     /gene="dgcZ"
                     /locus_tag="ACHLAG_003826"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFP0656971.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITLSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(5143..5535)
                     /gene="ydeI"
                     /locus_tag="ACHLAG_003827"
     CDS             complement(5143..5535)
                     /gene="ydeI"
                     /locus_tag="ACHLAG_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFP0656972.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFANTMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            5811..6329
                     /locus_tag="ACHLAG_003828"
     CDS             5811..6329
                     /locus_tag="ACHLAG_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFP0656973.1"
                     /translation="MNINSDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(6374..8419)
                     /gene="dcp"
                     /locus_tag="ACHLAG_003829"
     CDS             complement(6374..8419)
                     /gene="dcp"
                     /locus_tag="ACHLAG_003829"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFP0656974.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGLRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            8556..9302
                     /gene="ydfG"
                     /locus_tag="ACHLAG_003830"
     CDS             8556..9302
                     /gene="ydfG"
                     /locus_tag="ACHLAG_003830"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFP0656975.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            9391..10077
                     /gene="rspR"
                     /locus_tag="ACHLAG_003831"
                     /gene_synonym="ydfH"
     CDS             9391..10077
                     /gene="rspR"
                     /locus_tag="ACHLAG_003831"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFP0656976.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            10255..10458
                     /gene="ydfZ"
                     /locus_tag="ACHLAG_003832"
     CDS             10255..10458
                     /gene="ydfZ"
                     /locus_tag="ACHLAG_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFP0656977.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(10494..11954)
                     /locus_tag="ACHLAG_003833"
     CDS             complement(10494..11954)
                     /locus_tag="ACHLAG_003833"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP0656978.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(12043..13326)
                     /gene="ydfJ"
                     /locus_tag="ACHLAG_003834"
                     /pseudo
     CDS             complement(12043..13326)
                     /gene="ydfJ"
                     /locus_tag="ACHLAG_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
BASE COUNT         3746 a         3412 c         3046 g         3670 t
ORIGIN      
        1 aatagtttaa taatattaga ttcttactct tgcagtagtt tcatcgcaaa ttcaagaact
       61 gcctgtccgt tcatggtgcc gtagtctttc atctcgataa cggctacagg cattttcccc
      121 tgtgcaacgg cttcaatctc tggtttctga aagcgaactt gcgggccaag taaaacaacg
      181 tcaacttcgt tatttttaat tttcccttta gcctctgcaa tcgccaatgc ggaaatatta
      241 acttcaagtg aaatagcgtt agcatgatca atcatacgtt taaccagcat actggttgac
      301 atgcccgcag cgcaaactaa caatattttt ttcatatttt cccttactgg tatataacag
      361 attacattgt ttaaatatca tatcccgcag tagctatacg tcctggtaca ttgatcacag
      421 taatacagtg ataaagttgt tcaacatcag ggtgattatt aaacgactcg gccatacgat
      481 aaaccaatca ggactcaact ccttgttttc aatggctctt aaaaggtgca aaagtgattt
      541 ttcaaaagat tgcccgctta cttaaatccg aaatcaatgg caattcatgg catgttggtg
      601 atttgttgcc gtcagaagcg gaactcgctg ttcgttataa tgtttcgcgt aataccctgc
      661 gtaaggcatt atccttgctg gaaggcgaag gaattattca cagaaagcat ggttcaggaa
      721 catacattca aaaaaagaat tttgttgcac acattgatca catgaatagt ttcagtgaaa
      781 ttgcgcataa aagcggcaaa gaggcaggga gtcagattat gaaatttgaa gtgcaggatg
      841 cttcacccac tattgcgact gagctgaatt tagtgactgg tgagcaggtt tattacataa
      901 agagactgcg attcattgag gataatgcag cacaattaga agaaacgtgg atgtcagtag
      961 cacgttttcc tgatttaacc gtgtcacata tgcaaaaatc caaattttca tatattgaga
     1021 atgaatgcgg gatcaaaatc attggcacct ttgaaacttt ctcccctact tttcctaccc
     1081 cagaaatcgc cagtatttta cggatcagcc cacgagatcc catacttaaa attcagaccc
     1141 aggctgtgga cagtaactct attccgctgg attattcgtt actttacagc aatattttcg
     1201 agttccaggt aaagtacttt ttcccgcgat aaaagcagac gcacagagtg ctgacatcac
     1261 attctgtgcg tatggtttag cctctaacct ttaccgcttt acgccaccgc aagccaaata
     1321 cattgatata cagcccggtc ataatgagca ccgcaccgaa aaattgtaga cccgttaagc
     1381 gttcatccaa caataatgcc gcacttgcca gtccgactac gggcaccagt aacgataacg
     1441 gtgcaacccg ccaggtttca tagcgtccaa gtagtgttcc ccagatccca taaccaacaa
     1501 ttgtcgccac aaacgccaga tacatcagag acaaaatggt ggtcatatcg atagtaacca
     1561 gactgtgaat catggttgcg gaaccatcga gaatcagcga ggcaacaaag aagggaatga
     1621 ttgggattaa tgcgctccag attaccagcg acatcaccgc cggacgcgtt gagtgcgaca
     1681 tgatcttttt attgaagatg ttgccacacg cccaactaaa tgctgccgcc agggtcaaca
     1741 taaagccgag catcgccaca tgctgaccgt tcagactatc ttcgattaac accagtacgc
     1801 caaaaatcgc taaggcgatc cccgccaatt gtttgccatg caggcgctcc ccgaaagtaa
     1861 acgcgccaag cacgatagta aaaaacgcct gtgcctgtaa caccagcgaa gccagtccag
     1921 caggcatacc aaaattaatg gcgcagaaca gaaaggcaaa ctgcgcaaaa ctgatggtta
     1981 atccataccc cagcagcaaa ttcagtggta ctttcggtcg tgcgacaaaa aagatagccg
     2041 gaaaagcgac cagcataaag cgcaaaccgg ccagcatcag cggtggcatg ttatgaagcc
     2101 ccactttgat gaccacaaaa tttagccccc atacgaccac taccagtagc gccaacaccc
     2161 catcttttcg cgacattcta ccgcctctga atttcatctt ttgtaagcaa tcaacttagc
     2221 tgaatttact tttctttaac agttgattcg ttagtcgccg gttacgacgg cattaatgcg
     2281 caaataagtc gctatacttc ggatttttgc catgctattt ctttacatct ctaaacaaaa
     2341 cataacgaaa cgcactgccg gacagacaaa tgaacttatc cctacgacgc tctaccagcg
     2401 cccttcttgc ctcgtcgttg ttattaacca tcggacgcgg cgctacgctg ccatttatga
     2461 ccatttactt gagtcgccag tacagcctga gtgtcgatct aatcggttat gcgatgacaa
     2521 ttgcgctcac tattggcgtc gtttttagcc tcggttttgg tatcctggcg gataagttcg
     2581 acaagaaacg ctatatgtta ctggcaatta ccgccttcgc cagcggtttt attgccattc
     2641 ctttagtgaa taacgtgacg ctggtggtgc tcttttttgc ccttattaac tgcgcctatt
     2701 ccgtttttgc taccgtgttg aaagcctggt ttgccgacaa tctttcgtcc accagcaaaa
     2761 cgaaaatctt ctcaatcaac tacaccatgc taaacattgg ctggaccatc ggtccgccgc
     2821 tcggcacgct gttggtgatg cagagcatca atctgccctt ttggctggca gctatctgtt
     2881 ccgcgtttcc catgcttttc attcaaattt gggtaaagcg cagcgagaaa atcatcgcca
     2941 cggaaacagg cagtgtctgg tcgccgaaag ttttattaca agataaagca ctgttgtggt
     3001 ttacctgctc tggttttctg gcttcctttg ttagcggcgc atttgcttca tgcatttcac
     3061 aatatgtgat ggtgattgct gatggggatt ttgccgaaaa ggtggtcgcg gttgttcttc
     3121 cggttaatgc tgccatggta gttacgttgc aatattccgt gggccgccgc cttaacccgg
     3181 ttaacatccg cgcgctgatg atagcaggca ccctctgttt cgtcatcggt ctggttggtt
     3241 ttattttttc cggcaacagc ctgctattgt ggggtatgtc agctgcggta tttactgtcg
     3301 gtgaaatcat ttatgcgccg ggcgagtata tgttgattga ccatattgcg ccgccaggaa
     3361 tgaaagccag ctatttttct gcccagtctt taggctggct tggtgctgca attaacccat
     3421 tagtgagtgg cgtagtgcta accagcctgc cgccttcctc gctgtttatc atcttagcgt
     3481 tggtgatcat tgctgcgtgg gtgctgatgt taaaagggat tcgtgcaaaa ccgtgggggc
     3541 agcccgcgct ttgttgattt aagtcgaaca caataaaaat ttaattcagc cttcgtttag
     3601 gttacctctg ctaatatctt tctcattgag atgaaaatta aggtaagcga ggaaacacac
     3661 cacaccataa acggaggcaa ataatgctgg gtaatatgaa tgtttttatg gccgtactgg
     3721 gaataatttt attttctggt tttctggccg cgtatttcag ccacaaatgg gatgactaat
     3781 gaacggagat aatccctcac ctaaccggcc ccttgttaca gttgtgtaca aggggcctga
     3841 tttttatgac ggcgaaaaaa aaccgccagt aaaccggcgg tgaatgcttg catggataga
     3901 tttgtgtttt gcttttacgc taacaggcat tttcctgcac tgataacgga tcgttgacac
     3961 agtagcatca gttttctcaa tgaatgttaa acggagctta aactcggtta atcacatttt
     4021 gttcatcaat aaacatgcag cgatttcttc cggtttgctt accctcatac attgcccggt
     4081 ctgctcttcc aatgaccaca tccagaggct cttcaggaaa tgcgcgactg acacctgctg
     4141 tcacggtaat gttgatatgc ccttcagaaa gcgtgatggc atggttatcg actaactggc
     4201 aaattctgac acctgcacga catgcttctt catcattagc cgctttgaca ataatgataa
     4261 attcttcgcc cccgtagcga taaaccgttt cgtaatcacg cgtccaactg gctaagtaag
     4321 ttgccagggt gcgtaatact acatcgccga ttaaatgccc gtaggtatca ttaaccaatt
     4381 taaatcggtc aatatccaac aacattaaat aaagattcag aggctcagcg ttgcgtaact
     4441 gatgatcaaa ggattcatca agaacccgac gccccggcaa tcccgtcaaa acatccatat
     4501 tgctacggat cgtcagcaaa taaattttgt aatcggttaa tgccgcagta aaagaaagca
     4561 acccctcctg aaaggcgtcg aaatgcgcgt cctgccagtg attttcaaca atagccagca
     4621 ttaattcccg accacagtta tgcatatgtt gatgggcaga atccattagc cgaacgtaag
     4681 gtaattcatc gttatcgagt ggccccagat gatcaatcca ccgaccaaac tggcacagtc
     4741 cataagaatg gttatccgtt atttctggct tactggcatc tctcgcgacc acgctgtgaa
     4801 acatactcac cagccactgg tagtgggcat cgatagcctt attgagattt aacaagatgg
     4861 catcaatttc cgttgttttc ttgatcattg ccactccttt ttcacagttc cttgtgcgcg
     4921 ctattctaac gagagaaaag caaaattacg tcaatatttt catagaaatc cgaagttatg
     4981 agtcatctct gagataacat tgtgatttaa aacaaaatca gcggataaaa aagtgtttaa
     5041 ttctgtaaat tacctctgca ttatcgtaaa taaaaggatg acaaatagca taacccaata
     5101 ccctaatggc ccagtagttc aggccatcag gctaatttat ttttatttct gcaaatgagt
     5161 gacccgaacg acggccggcg cgcttttctt atccagactg ccgctaatgt tgatcatctg
     5221 gtccggctga acttctcgtc catcaaagac ggccgcagga ataacgacat taatttcacc
     5281 gctcttatcg cgaaaaacgt aacggtcctc tcctttgtga gaaatcaaat taccgcgtag
     5341 tgaaaccgaa gcgccatcgt gcatggtatt tgcgaaatca acggtcattt tttttgcatc
     5401 atcggttccg cgatagccat cttctattgc atgaggcggc ggtggcgctg catcctgttt
     5461 taaaccgccc tggtcatctg ccaacgcata aggcatgaca agaaaacttg ctaatacaat
     5521 ggcctgaaat ttcatactga ctccttaatt gcgtttggtt tgacttatta agtctggttg
     5581 ctatttttat aattgccaaa taagaatact gccaattgtt ataaggcatt taaaatcagg
     5641 caactagctg tcaaatattc agagaattta actcactaaa gttaagaaga tcaaatagtc
     5701 ttaaatatat tttcagaata atcggattta tatgtttgaa aattattata ttggacgagc
     5761 atacagaaaa agcaaatcac ctttacatat aaaagcgtgg acaaaaaaca gtgaacatta
     5821 atagcgataa aattgtacaa cttgcagata ccgacactat tgaaaacctg acatccgcgt
     5881 tgagtcaaag acttatcgcg gatcaattac gcttaactac cgccgaatca tgcaccggcg
     5941 gtaagttggc tagcgccctg tgtgcagctg aagatacacc caaattttac ggtgcaggct
     6001 ttgttacttt caccgatcag gcaaagatga aaatcctcag cgtaagccag caatctcttg
     6061 aacgatattc tgcggtgagt gagaaagtgg cagcagaaat ggcaaccggt gccatagagc
     6121 gtgcggatgc tgatgtcagt attgccatta ccggctacgg cggaccggag ggcggtgaag
     6181 atggtacgcc agcgggtacc gtctggttcg cgtggcatat taaaggccag acctatactg
     6241 cggttatgca ttttgctggc gactgcgaaa cggtattagc tttagcggtg aggtttgccc
     6301 tcgcccagct gctgcaatta ctgctataac caggctggcc tggcgatatc tccaggacag
     6361 ccattggtgg tgtttatatg ttcaagccac gatgttgcag catcggcata atctgaggag
     6421 ccttaccgcg ccattgtcga tacaggcgtt ccagatcttc gctgttacct ctggaaagga
     6481 tcgcctcgcg aaaacgcagc ccattttcac gcgttaatcc gccctgctca acaaaccact
     6541 gataaccatc atcggccaac atttgtgtcc acagataagc gtaataacct gcggcatatc
     6601 cgccaccaaa aatatgggcg aaataactgc tgcgatagcg tggcggtata gcaggaagat
     6661 ccatattttc cgccaccagc gcccgcaatt caaaatcatc gacatcctgc attgcttcgt
     6721 tttcttccag gcaatgccag cgcatatcga gaagtgcggc gctaagcagt tcgctcatct
     6781 catacccttt gttgaacagg ctggcattac gcattttctg ttgcagttcg tcaggcattg
     6841 ctgccccgct ctgataatgc cgggcgtagc gagcgaatac ctgcggatgc gttgcccagt
     6901 gttcgttgat ttgcgacgga aattcgacaa aatcacgcgg cgtgttggtg ccggaaagcg
     6961 tggcataacg ctggcgggca aaaaggccgt gcagcgtatg accaaattca tggaataagg
     7021 ttatgacatc atcccagagt aacaacgcag gctcaccggc agcgggtttc tgataattgc
     7081 aaacgttata aattaccgga tgcgtttcat taagcgttga ttgctcaaca aaattgccca
     7141 tccatgcacc gccgcttttt gaatcacggg cgaagaaatc accgtaaaat aacgccagtc
     7201 ccacgccatt atgatcaaaa atttcccaca cacgaacgtc aggatggtag acaggaatat
     7261 caaaacgttc gacaaactta ataccgaaga gctgattcgc ggtccagaat acaccttcat
     7321 tcaacaccgt gtttaattca aaatatggct tgagctgcgc ctcatcaaga tcaaatttct
     7381 cccgccgtac ctgttcggca taaaatgccc agtcccacgg ctgcgcgcta aacccgcctt
     7441 gctgcttatc gataaccgcc tgtatggagg ctaattcatc gctagcacgt tgacgcgccg
     7501 ctggaacaat ttcccgcata aagttgagtg ctgcttcagg tgtttttgcc atctgatcgg
     7561 cgattttcca tgcggcataa tgaggaaaac caagcagttt cgcctgctgt gcgcgaatct
     7621 ctaccagacg ttgaatgata gcgcgggtat cattgccatc atttttttcc gctcgcgtcc
     7681 agcccgcaat aaacagtttt tcacgcgtcg cacgatcgcg catttcggca agcgccggtt
     7741 gttgggtggt attcagcagc ggaatcagcc atttgttatc cagacctttc tcgcgagccg
     7801 cctctgccgc cagcgcaatc tcttgctcac tcattcctgc cagctgcgcg atatcgttca
     7861 caaccagacc gccggattta tttgctgcaa gtaaccgctg gttaaactgg ctggtcaggg
     7921 tcgcagcttc tgtattcagt acttttaatt ttgctttatc agcttgcgca agtttggctc
     7981 cggcaaggac aaaacgttga tgaataacct ccaccaggcg gatggattca ctatcaagcc
     8041 ccagggattc acggcgctgc cagacagcat ctacccgcgc gaataattca ccgttcagat
     8101 agatatcatt agccagttcc gccagttcag cggaaaactg ttcatcaaga cgctgtaatt
     8161 catcattggt atgcgccgca gtcatcgcaa aaaagacgct ggtaacgcgg gtaagtaatt
     8221 ctccgctttg ttccagtgcc agaatagtat tgttgaaatc aggcgtttgc gggttaagcg
     8281 cgatggcagc aatttctgcc cgcttttgct gcattccctc atcgaatgcc gggcgatagt
     8341 gatgattggc aatttgatca aaatgaggag ccagatacgg cagtgtgctt tgcacaagga
     8401 aaggattcat tgttgtcatt ttcttctcct gaacgcgagg tgttccatag cgtaggctta
     8461 ctgataacgg agtgcaatct tgcaaaccag tattacccgc tcttaagcat cacgtgctat
     8521 gttattgaca cacaaaagcg ttgaggaaca gtgagatgat cgttttagta actggagcaa
     8581 cggcaggttt tggtgaatgc attactcgtc gttttattca acaagggcat aaagttatcg
     8641 ccactggccg tcgccaggag cggttgcagg agttaaaaga cgaactggga gataatctgt
     8701 atatcgccca actggacgtt cgcaaccgcg ccgctattga agagatgctg gcatcgcttc
     8761 ctgccgagtg gtgcaatatt gatatcctgg taaataatgc cggcttggcg ttgggcatgg
     8821 agcctgcgca taaagccagc gttgaagact gggaaacgat gattgatacc aacaacaaag
     8881 gcctggtata tatgacgcgc gccgtcttac cgggtatggt tgaacgtaat catggtcata
     8941 ttattaacat tggctcaacg gcaggtagct ggccgtatgc cggtggtaac gtttacggtg
     9001 cgacgaaagc gtttgttcgt cagtttagcc tgaatctgcg tacggatctg catggtacgg
     9061 cggtgcgcgt caccgacatc gaaccgggtc tggtgggtgg caccgagttt tccaatgtcc
     9121 gctttaaagg cgatgacggt aaagcggaaa aaacctatca aaataccgtt gcattgacgc
     9181 cagaagatgt cagcgaagcc gtctggtggg tgtcaacgct gcctgctcac gtcaatatca
     9241 ataccctgga aatgatgccg gttacccaaa gctatgccgg actgaatgtc caccgtcagt
     9301 aatttttata cccggcgtaa ctgccgggtt attgcttgtc acaaaaaagt ggtagactca
     9361 tgcagttaac tcactcacaa gcaagaacga atgaccgtcg aaacgcaact taatcccaca
     9421 cagcctgtca atcagcagat ttatcgtatt cttcgtcgcg acattgtcca ttgcctgatt
     9481 gcaccaggca caccgttgtc ggaaaaagaa gtttctgttc gtttcaatgt gtcacgccag
     9541 ccggttcgtg aagcctttat taaactggcg gaaaacggcc tgattcaaat tcgtccgcag
     9601 cgtggcagct acgtcaacaa aatttccatg gcccaggtgc gaaacggcag ttttatccgt
     9661 caggccattg agtgcgcggt ggcgcgtcgg gcggcgagca tgattaccga aagccagtgc
     9721 taccaactgg aacaaaatct tcaccagcaa cgcattgcca ttgagcgcaa gcaactggat
     9781 gatttttttg aacttgatga caacttccat caactcctga cgcagattgc cgactgtcaa
     9841 ctggcgtggg ataccattga gaacctgaaa gcgaccgttg atcgcgtgcg ctatatgagt
     9901 ttcgaccatg tttctccacc agaaatgctg ttacgccagc atcttgatat tttctctgcc
     9961 ctgcaaaaac gtgatggcga tgcggtagaa cgtgcaatga cgcaacattt gcaggaaatc
    10021 agcgaatccg tgcgccagat ccgccaggaa aacagcgact ggtttagcga agagtaattc
    10081 atttactctc atcccatccg ggatgagagg cttttccccc cgccttacgg ctcatgcatg
    10141 catcaaaaaa gatgtgagct tgatcaaaaa caaaaaatat ttcactcgac aggagtattt
    10201 atattgcgcc cgttacgtgg gcttcgactg taaatcagaa aggagaaaac acctatgacg
    10261 acctacgatc gtaaccgtaa cgcaatcacc actggcagcc gtgttatggt tagcggcacc
    10321 ggtcacactg gcaagatcct gtcgattgat actgaaggtc tgaccgctga gcaaatccgc
    10381 cgcggaaaaa ccgtagttgt tgaaggttgt gaagagaaac tggcacctct ggacctgatt
    10441 cgtctcggca tgaactaagt gtgtgaatgc cgccgatggc ggcattgctt tttttacttc
    10501 acggaatatt tcgccacggt cgctttcgcg ccatgcgcta ataaagacaa gtacgcttcc
    10561 gtcacttttg cagtaaacaa gctattgtct ggcaaatcat caccaaagat tgccttaatc
    10621 gccagcaatg actggacgcg cgctttccct tcggcactac tttgtacagc cttctgaata
    10681 acaggtaaca gtgggtcact gatttctatc ggatttccct gttcatcaac accaccgaca
    10741 taacgcatcc aacccgcgac gcccagcgcc agcagatcga acttgctgtc atgcgccaga
    10801 tgccagcgaa cagaatccaa catccgctgt ggcaatttct ggctaccatc catcgcaatc
    10861 tgccaggttc gatgacgtaa cgccggattg ctatagcgtt caattaatcg gttagcgtaa
    10921 tcttgcaaat caacgccctg cactttcagc gtcggcgctt gttcctgcaa catcaagcca
    10981 tacgccgcat aacgataatg ttcatcttcc atgcagtcat taatgtgctg atatccggca
    11041 agataaccca aatacgccag gaatgaatga ctgccgttga gcatgcgcaa cttcatctct
    11101 tcataaggca gcacatcact aaccagttcg gctcccgctt tttcccattc tggacgtccg
    11161 gcaacaaagt tatcttctat tacccactgg cggaaaggtt cacaggcaac gcccgcagga
    11221 tcgcccacac cggtaagttg ttcgattttc gccagcgtat cctctgtcac tgcgggcaca
    11281 atacggtcca ccattgttga tgggaaagtc acgttatctt cgatccattg tgccagtttt
    11341 acatcaacag ctttcgcgta tgaagtgaca acgtcacgca taacatgacc gttttctggc
    11401 atgttgtcac atgacatgac ggtaaatgcg ggaagtcctg ccgctttacg gcgggccagc
    11461 gcctcaacaa tcacccctgt tgctgttttc ggctggtggg gattttgcac gtcggcagcg
    11521 accatcgggt gatcgagcat taactgtccg gtcgccggag agtggaaata ccctttttcg
    11581 gtgattgtca gagagacaat cgcgatttgc ggttcacaca tcgcagccaa cacggtttct
    11641 aagccatcca tctgtacgtg caaggctttt ttaacgacgc caacgacgcg agccgtccac
    11701 gcatcggccg acatttccgc aacggtataa agattatctt gctgttgtaa atcggcaatt
    11761 tgctgttcgc cgccgattaa gttgacctca taatatcccc agtcactgaa atgttctgta
    11821 gcaagaatat cggcatacac gccctgatgc gcacggtgaa atgcaccaaa gcctaaatga
    11881 ataattcttg gggccaggtt attacgatca taaacaggga gtgtcgcttt tgctgataac
    11941 aaattatttc ccataacaat tccttaaatt taaatatggc aagctatatg ttttgttata
    12001 tgaataaaaa tcccctctcc ggtaagagaa gggattgagg gtttacagac ttctggaagg
    12061 ttgcgcagct cttacaacac gcggttgatc ttccgcagcg tcttccagcg cacttaaatc
    12121 acggtctttc acctctggca ttttcagcgc agagattaaa ccaattactg aatatgccat
    12181 gatcataatg gcgatcggat accaggattc cgtcatggtg cagaaaatac ccgccaggat
    12241 aggaccaaaa ccggaagcga taataccacc aatttcttta gaaatagcca tccgggtaaa
    12301 gcggttttta cagccgaaca tttctgccat ggtaatgttt tccagagcaa ataatcccag
    12361 caccgcacag ttatgaatca caatcagtgc aaccataatg gtgctcgggg catagctttt
    12421 atctacaatg atagaaagca ttggccatgc cagcacaatc gcggaggtat tcataataat
    12481 atacgggatc cggcgaccaa ttttatcgga taaccaacca aggaacggaa tggtcataaa
    12541 gccgagaatc aaactaatca tcaatgcatc tgttggaatt gctttgttaa acaataacgt
    12601 ctgcactaaa tagcctgcaa ggaaagtctg aattaacccg gagttacccg cctgaccaaa
    12661 acgcagccct gttgccagcc agaaggattt gctctggaac atgctaccag caggtgcagg
    12721 ttttgctgtg ggttggttgc tgtcattaac cttctcaaag accgggcttt ctttcagatt
    12781 catacgtaac cagatagcaa agaccatcac gacaacgctc gccaggaacg gtatacgcca
    12841 tccccacgcc agcagttcct ctttactgag aatgaagaac ataaaggccc agattgccgt
    12901 tgcgctcaag gttccgcagt tagttcccat agctacaaat gaggagataa ttccgcgctt
    12961 accttttggt gcatattccg ccagcatcgt accggcaccg gaaatttccg cacctgcacc
    13021 caacccctga ataatacgca gcgtcaccag caagatgggg gcaaaaacac caatctgtgc
    13081 ataggtcggt aacacaccaa ttaaggtggt acagatcccc atcatggtga tggtaataaa
    13141 gagcactttt ttacgcccta ttctgtcgcc cattttgccg aaaataaatg ctccgacaat
    13201 acgcgccaca taacctgcac cgtaggttcc cattgccaaa attaacgcca ttgccgttga
    13261 tgattcagga aaaaatattt catgaaacac taacgctgcg ccgagcgaat ataactggaa
    13321 atccataatt cacaggtgtt ttttcccatc ctgtggtttc cttggcgttt tctaggtttt
    13381 ttcagatagt tgcatttttt aaaaagcatc ctaagttcga tctcagtgtc tatctggggc
    13441 ctatttctgt cccatatatg ccccaaaaaa actccccaac agataagtag ttttttcatg
    13501 gatttacgcg taaaatcaat aacggctgga aatcattcaa tactcgcact atcgaaaaat
    13561 ttaccagcca accgcagcac gttcttgcat aaggcgtgtc tgcggttttt caactattca
    13621 gatacatcac tcccatcaca ttcattcctc cgcatcaaag gcatataggc tatatcacct
    13681 tgatattttt cttcttcaga taaaaactgt tatctatgta tacttttaaa cccaatccgt
    13741 gtagagtctc tgcataagat agtttgcagt tgccacttca gcttgcgcca taaaccgcct
    13801 gatttttgcc gccacctgtt agcattcctg tatacctgaa acgacaatgt ttatctacga
    13861 actttaagaa cacc
//
LOCUS       DAZGVQ010000130        13785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000130 DAZGVQ010000000
VERSION     DAZGVQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.131"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..849
                     /gene="hlyE"
                     /locus_tag="ACHLAG_003835"
                     /pseudo
     CDS             1..849
                     /gene="hlyE"
                     /locus_tag="ACHLAG_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            complement(1056..1502)
                     /gene="ycgN"
                     /locus_tag="ACHLAG_003836"
     CDS             complement(1056..1502)
                     /gene="ycgN"
                     /locus_tag="ACHLAG_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFP0656979.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1594..2253)
                     /gene="ycgM"
                     /locus_tag="ACHLAG_003837"
     CDS             complement(1594..2253)
                     /gene="ycgM"
                     /locus_tag="ACHLAG_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFP0656980.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLATRV
                     L"
     gene            complement(2325..2618)
                     /locus_tag="ACHLAG_003838"
     CDS             complement(2325..2618)
                     /locus_tag="ACHLAG_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFP0656981.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            2860..3261
                     /gene="pliG"
                     /locus_tag="ACHLAG_003839"
     CDS             2860..3261
                     /gene="pliG"
                     /locus_tag="ACHLAG_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFP0656982.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3381..3749)
                     /gene="ycgJ"
                     /locus_tag="ACHLAG_003840"
     CDS             complement(3381..3749)
                     /gene="ycgJ"
                     /locus_tag="ACHLAG_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFP0656983.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            4269..4964
                     /gene="minC"
                     /locus_tag="ACHLAG_003841"
     CDS             4269..4964
                     /gene="minC"
                     /locus_tag="ACHLAG_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFP0656984.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            4988..5800
                     /gene="minD"
                     /locus_tag="ACHLAG_003842"
     CDS             4988..5800
                     /gene="minD"
                     /locus_tag="ACHLAG_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFP0656985.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5804..6070
                     /gene="minE"
                     /locus_tag="ACHLAG_003843"
     CDS             5804..6070
                     /gene="minE"
                     /locus_tag="ACHLAG_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFP0656986.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6442..6786)
                     /locus_tag="ACHLAG_003844"
                     /pseudo
     CDS             complement(6442..6786)
                     /locus_tag="ACHLAG_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6821..6940)
                     /locus_tag="ACHLAG_003845"
     CDS             complement(6821..6940)
                     /locus_tag="ACHLAG_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP0656987.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(6901..7086)
                     /gene="ymgJ"
                     /locus_tag="ACHLAG_003846"
     CDS             complement(6901..7086)
                     /gene="ymgJ"
                     /locus_tag="ACHLAG_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFP0656988.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            7187..7360
                     /gene="ymgI"
                     /locus_tag="ACHLAG_003847"
     CDS             7187..7360
                     /gene="ymgI"
                     /locus_tag="ACHLAG_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFP0656989.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            7362..7706
                     /gene="ymgG"
                     /locus_tag="ACHLAG_003848"
     CDS             7362..7706
                     /gene="ymgG"
                     /locus_tag="ACHLAG_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFP0656990.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            7710..8045
                     /gene="ymgD"
                     /locus_tag="ACHLAG_003849"
     CDS             7710..8045
                     /gene="ymgD"
                     /locus_tag="ACHLAG_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFP0656991.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(8102..10423)
                     /locus_tag="ACHLAG_003850"
     CDS             complement(8102..10423)
                     /locus_tag="ACHLAG_003850"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP0656992.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAVDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(10540..10749)
                     /locus_tag="ACHLAG_003851"
     CDS             complement(10540..10749)
                     /locus_tag="ACHLAG_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0656993.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            complement(11149..11367)
                     /gene="ymgF"
                     /locus_tag="ACHLAG_003852"
     CDS             complement(11149..11367)
                     /gene="ymgF"
                     /locus_tag="ACHLAG_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFP0656994.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
     gene            complement(11499..13022)
                     /gene="pdeG"
                     /locus_tag="ACHLAG_003853"
     CDS             complement(11499..13022)
                     /gene="pdeG"
                     /locus_tag="ACHLAG_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0656995.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(13354..13602)
                     /gene="ymgC"
                     /locus_tag="ACHLAG_003854"
     CDS             complement(13354..13602)
                     /gene="ymgC"
                     /locus_tag="ACHLAG_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFP0656996.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            complement(13715..>13785)
                     /gene="ariR"
                     /locus_tag="ACHLAG_003855"
                     /gene_synonym="ymgB"
     CDS             complement(13715..>13785)
                     /gene="ariR"
                     /locus_tag="ACHLAG_003855"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFP0656997.1"
                     /translation="ILKADVIRKTLEIVLRYTADDM"
BASE COUNT         3945 a         3204 c         2958 g         3678 t
ORIGIN      
        1 tggagcatta gatctttata ataaatatct cgatcaggtc atcccctggc agacctttga
       61 tgaaaccata aaagagttaa gtcgctttaa acaggagtat tcacaggcag cctccgtttt
      121 agtcggcgat attaaaacct tacttatgga tagccaggat aagtattttg aagcaaccca
      181 aacagtgtat gaatggtgtg gtgttgcgac gcaattgctc gcagcgtata tttttctatt
      241 tgatgagtac aatgagaaga aagcatccgc ccagaaagac attctcatta aggtactgga
      301 tgacggcatc acgaagctga atgaagcgca aaaatccctg ctggtaagct cacaaagttt
      361 caacaacgct tccgggaaac tgctggcgtt agatagccag ttaaccaatg atttttcaga
      421 aaaaagcagc ttttccagtc acaggtagat aaaatcagga aggaagcgta tgccggtgcc
      481 gcagccggtg tcgtcgccgg tccatttgga ttaatcattt cctattctat tgctgcgggc
      541 gtagttgaag ggaaactgat tccagaattg aagaacaagt taaaatctgt gcagaatttc
      601 tttaccaccc tgtctaacac ggttaaacaa gcgaataaag atatcgatgc cgccaaattg
      661 aaattaacca ccgaaatagc cgccatcggg gagataaaaa cggaaactga aacaaccaga
      721 ttctatgttg attatgatga tttaatgctt tctttgctaa aagaagcggc aaaaaaaatg
      781 attaacacct gtaatgagta tcagaaaaga cacggtaaaa agacactctt tgaggtacct
      841 gaagtctgat aagcgattat tctctccatg tactcaaggt ataaggttta tcacatttaa
      901 aaacacagac gttttttaaa gattgagagt tacctgttga taataaggta tcgggtaact
      961 ctcattatga cagagcgctt tcaaaaacag ggctacgaaa tggcgagcat ccagacgtat
     1021 attaagaaag cccccatcaa catctattaa atttatcact gcgcccagtc aggtttattt
     1081 aagatatgat cctgccagtc aatcacttct gattctttca ctgcgatatg acgcacagag
     1141 atacgttcac catgcattgc cgctttcgaa ccagtaagta gcggatgcca cgcaggtaaa
     1201 tctttacctt ccgccagcaa acgataagcg caggtcattg gcagccattc gaatgttggc
     1261 agattttcac gggttaattt aatgcagtcg ggttcaaact cgaaacgacg ttcgtagttc
     1321 cgacattgac aggttttaat attgagctgg cgacaggcga cgttagtgaa gtagatttcg
     1381 tcggtgtctt catccatcag tttatgcagg caacactgac cgcaaccatc acataacgac
     1441 tcccattccg catcgctcat ttcgtccagg gttttacttt gccagaaagg tacatcgctc
     1501 atcaggtgtt ccgccattac gttaaaacgc accttataac cagtctggca cagcgatgca
     1561 agttttgccg ccgctttcag gcggcaaaaa gtattacaaa acgcgagttg ccaaagaatg
     1621 cccatcgaaa gtgactgtca gctcatcacc gctttgcaac gggccgacgc catcaggcgt
     1681 gcctgtcagc acaacgtcac cggccttgag ggtaaaaaac ttgctcatat aagcgatcag
     1741 cggaacaatt ttatggatca tgtccgcagt cgtaccttgc tgacgttgct cgccgtttac
     1801 gctcaggccc agcgttgtat tttgcgggtc gccggtaaat tccgccgcgg gaataaaccc
     1861 ggaaagcgga caagagttat caaacgcttt agccttttcc cacggctgcc cggctttctt
     1921 catttttccc tgaacatcac gcaatgtcag atcgagcgcc acgccataac cggcaatggc
     1981 tttgcggaca tgctcttccg tagcctgacg cagcgtcgcg ccaatcaaca ccgccagttc
     2041 gacttcatga tgaactgaac cgaaatcgga tgggatcgcc aatggctgcc gcagatcgca
     2101 cagtgccgtt tctggtttaa taaacagcac tggctcttcg ggcactgcgc tgcccatctc
     2161 tttaatatgt ttggcatagt tactgccaac acagactact ttactcaccg gataatccag
     2221 cagcgcacct tgccagttgt gatgttgata cataacgttc ccctcacctt atcgtggata
     2281 tgaaaacccg cgccgacaaa gataatgcca ccgccggata tcggttattt gttagtgtcg
     2341 tctgttttct gccccatgac ggaaagatgt tgcttcagca aatcttcggg tggtggcggt
     2401 aactgcaaat agtaaccttg ctcggttaat gccagtttaa ccttttcaat atcagcattg
     2461 accagtttct tccgcccatc caacggcaga atcatcgcta actgaggctg accaaaacct
     2521 ttcatcagtt cctcaggaac acgcgaaaaa tcgtcttttt tttcgacata taaataggtc
     2581 tggtcacgct tgctgcttcg ataaatcaca caaaacatac ttttactctt taagatgcca
     2641 ggttttggca taagctaaca tcaatgaaac ccgcgccaca agccagccag ctgtagattt
     2701 ctacggtttg ttgagtccat gcccctttat aagtaatccc cccggcaaaa aatgccgcgc
     2761 cgggatttta ccgaacgcag aaaagccact ggaattctcc cgtaagggat tgttatatta
     2821 taacagttca tcgtactcat tctgaacagg agactaccaa tgaaaatcaa gagcatcagt
     2881 aaggctgtat tgctgcttgc actgctaacc tccacgagct ttgctgcggg taagaatgtc
     2941 aatgttgagt tcagcaaagg ccatagcagc gctcagtact ccggcgaaat aaagggatac
     3001 gattacgata catatacctt ctatgccaaa aaaggtcaga aagtgcatgt gagtatctct
     3061 aatgaaggcg ctgatactta cctcttcggg ccaggaatcg atgattcagt tgatttatcc
     3121 agatattcac cagaactcga tagtcacgga caatactcac tccctgcttc tggcaaatat
     3181 gagctgcgag tgctgcaaac ccgaaacgac gcccgtaaaa acaaaactaa aaaatataac
     3241 gtcgatattc aaattaagta aatgtcaacc gacggggact ctccccgtca taaacgttaa
     3301 cgccccactc ctctctgtcg ctcaagaggt taatgacgaa tagcagacaa tgccgaatag
     3361 aacataatgt aaacaaggca tcactcacga cacataaata acatctttgt ggtgatttga
     3421 ttgatttttc cactccgctt accatccaca ccaaaataac gatcatcacg acataatttt
     3481 tctttaacat cgcaaaatac accgttagcg aatgtaaatt cgctgggatc gtagccttgt
     3541 aatgattgta agttttcagc ggctttttcg ccgagatatt tccttgttaa ctctggggaa
     3601 atgccatctg agttcacaca aatatacggg tcgcaaacaa cgccgcacgc cggagattga
     3661 agtccgccag cgtcatgtcc agacgccatt gatgagaaca tcatccccac acctgacaat
     3721 ccgatataaa aaatacgcat catattcata gcacatcctg ataatccaac cgtacaacac
     3781 ttatgctcat ttcatcatca atccgtaatc aaaaccgctg acctggacga gttacgatga
     3841 cctgacagaa gtatcagacg tcttctgcgg tttctatgat agagcaattc tgtggcggat
     3901 attaagataa cgggtatctg ttctttaatg atgccgcaaa aaatggaaag ttaagaaagc
     3961 ctggcgtggt gaaaaaatac acttgcctca atactggata tttaaacagg tctggagatg
     4021 ctgtcacgat tagggtgcgg aaaaaggaag cgtaaataag aacaatcaaa agtacgacgg
     4081 caatgggttg attgacaagg gtatttttta agctatgaat cagcgccatt tatcacagaa
     4141 tagactttta ctctgaataa atgggagggt gacttgcctc aatataatcc agactataac
     4201 atgccttata gtcttcggaa catcatcgcg cgctggcgat gattaatagc taattgagta
     4261 aggccaggat gtcaaacacg ccaatcgagc ttaaaggcag tagcttcact ttatctgtgg
     4321 ttcatctgca tgaggcagaa cctaaggtta tccatcaggc gctggaagac aaaatcgctc
     4381 aggcccccgc atttttaaaa catgcccccg ttgtactcaa cgtcagtgca ctggaagacc
     4441 cggtaaactg gtcagcgatg cataaggcgg tttcggcaac cggtttgcgg gttattggcg
     4501 taagtggctg caaagatgcg caacttaaag ccgaaattga aaagatgggg ctgcctatcc
     4561 tgacggaagg aaaggaaaaa gcgccgcgtc cagctcccgc accacaggct ccagcgcaaa
     4621 atacaacgcc ggtcacaaaa acgcgtttaa tagatacccc ggtgcgttcc ggtcagcgta
     4681 tttatgctcc acaatgtgat ctgattgtta caagccacgt tagcgctggg gccgaattga
     4741 ttgccgatgg gaacattcat gtctatggca tgatgcgcgg tcgtgcgctt gcaggggcaa
     4801 gtggtgaccg ggaaacgcaa atattttgta cgaacctgat ggcggaactg gtgtccatcg
     4861 caggtgaata ctggctgagt gatcaaatcc cagcagaatt ttatggcaaa gcggcgcgac
     4921 tgcagttagt cgaaaacgct ttgaccgttc aaccgttaaa ttgatccctt tttaacaagg
     4981 aatttctatg gcacgcatta ttgttgttac ttcgggcaaa gggggtgttg gtaagacaac
     5041 ctccagcgcg gccatcgcca ctggtttggc ccagaaggga aagaaaactg tcgtgataga
     5101 ttttgatatc ggcctgcgta atctcgacct gattatgggt tgtgaacgcc gggtcgttta
     5161 cgatttcgtc aacgtcattc agggcgatgc aacgctaaat caggcgttaa ttaaagataa
     5221 gcgtactgaa aatctctata ttcttccggc atcgcaaaca cgcgataaag atgccctcac
     5281 ccgtgaaggg gtcgccaaag ttcttgatga tctgaaagcg atggattttg aatttatcgt
     5341 ttgtgactcc ccggcaggga ttgaaaccgg tgcgttaatg gcactctatt ttgcagacga
     5401 agccattatt accaccaacc cggaagtctc ctcagtacgc gactctgacc gtattttagg
     5461 cattctggcg tcgaaatcac gccgcgcaga aaatggcgaa gagcctatta aagagcacct
     5521 gctgttaacg cgctataacc caggccgcgt aagcagaggt gacatgctga gcatggaaga
     5581 tgtgctggag atcctgcgca tcaaactcgt cggcgtgatc cctgaggatc aatcagtatt
     5641 gcgcgcctct aaccagggtg aaccggtcat tctcgacatt aacgccgatg cgggtaaagc
     5701 ctacgcagat accgtagaac gtctgttggg agaagaacgt cctttccgct tcattgaaga
     5761 agagaagaaa ggcttcctca aacgcttgtt cggaggataa gttatggcat tactcgattt
     5821 ctttctctcg cggaagaaaa acacagccaa cattgcaaaa gaacggctgc agattattgt
     5881 tgctgaacgc cgtcgcagcg atgcagaacc gcattatctg ccgcagttgc gtaaagatat
     5941 tcttgaggtc atttgtaaat acgtacaaat tgatcctgag atggtaaccg tacagcttga
     6001 gcaaaaagat ggcgatattt ctattcttga gctgaacgtg accttaccgg aagcagaaga
     6061 gctgaaataa gcccgctgta aaaacgcatt tatcttcaag gcagagtcat ttctgccttg
     6121 agtttttcat ccctctcatc cacgttgtgg taaagcggcg agtattcttg ctgatactcc
     6181 tcattgctat ttccacctcc ccctttacac cttaaggctg ttcccacgca cagaacaaaa
     6241 tgtttatgcc tggttgagta aataaccctg ttgttagtgt ggatatactg ggagatgata
     6301 accccgcaat ttcattattc attgttctcc cataggatga ggtttcccgc cgtaactggc
     6361 gggctttttt tgccccagaa ttttctcttt caacatcctg taaacgaaaa ctgcgccgaa
     6421 gcgcagttta atcgttgatg gtcagaacga ccagttcaca ccagccaccg cgttccacgg
     6481 ggactccaca ccggcaccat ggctataccc caccccgaaa tgcccgctta acgtactgct
     6541 gaatgaggct ttaatacctg cctggtatat tccacgtctg cccgacaaat cattgacaaa
     6601 attaccgtca ctattcactt tcacccgatt atcatcgaca aattctttgc gcacagccgc
     6661 cttcaggcag ggttcaattt ccataccgtt ccccagacgc atgttgtaac tcagcgttgc
     6721 acccagttca cgatatatac tgcgggtatc gactgatttc gattccatgc cattggataa
     6781 atgatagtag cgtctaagtg acctaagctc tgctgcaaca tcagcccgca ggcaccagac
     6841 caggggattc atcctgaaga gacagcgcag gtgtattgtg ttcaccgctc accaaagaca
     6901 tcatgatgaa atgatgatat cccgcataag aaagaggcat tttttacacg cagtgcgctg
     6961 aagtgtagtt ggataaaaaa tcactctttt cagataatac tgtcgtacgc ttatctttcg
     7021 acaatgaggc cgtcggcaaa ataaaatgac taatgtaatc gttgctgatg aatatcttct
     7081 gctcacataa aaatcacaca atgatattga taaatcagat tatcttacct ttacaacatt
     7141 gcgttaacag tcaatgacac ttcttgaata aaaatattgt caatcaatga gttattcctc
     7201 atttaagatt attctcattc acgtaaaaaa cataattcct atcatcacag ccacgctcat
     7261 tttgaattat ctgaataata gcgagcgttc tttagttaag cagatcttaa ttgaagacga
     7321 aattattgtg tgtgcaactt acttaatacc ggacatctaa gttgaagaaa aaaatacttg
     7381 ccttcgggct tatctcagca ttgttctgct ccacgccagc gatggcggat atgaaccgca
     7441 ccacgaaagg tgcattgcta ggcgcaggcg ttggtttact tactggcaat ggtgttaatg
     7501 gcgtactgaa aggtgcagct gttggcgctg gtgttggtgc agtaacagaa aaaggccgcg
     7561 acggtaaaaa tgcgcgtaaa ggtgcaaaag tgggtgccgc cgttggtgct gtcactggcg
     7621 ttctgaccgg caatggcctc gaaggggcga ttaaaggtgc cgtgattggc ggtacgggtg
     7681 gtgcaattct gggtaaaatg aaataaggaa tgatgatgaa aaaatttgct ttactggcgg
     7741 gcctgtttgt tttcgcacca atgacctggg cgcaggacta caatattaaa aacggtttgc
     7801 cgtcagaaac ctatattact tgcgcagaag ccaacgaaat ggcaaaaact gacagcgcac
     7861 aggttgcaga aattgttgcg gtaatgggta atgccagcgt tgccagccgt gatttaaaaa
     7921 ttgagcaatc tccggaactg agcgccaaag tggtagaaaa actgaatcag gtttgcgcta
     7981 aagacccgca aatgctgttg atcacggcaa ttgatgacac catgcgtgct atcggcaaaa
     8041 aataatattt catatgtacc gcgtcgatga atacgacgcg gttttttagc taacggcggg
     8101 atcagaactt atatttcagg cccaccatca ctgcggtgtc attgtagcca ttatcaccca
     8161 gctgcacgcc cacattcccc cagacgctgg ccgctggatt caggttgcca tttacgccaa
     8221 gtttaatttc ccccaaatta ctcgccccat cttgttcgac tttcacaccg ttcattgaga
     8281 cagcaaaatc tttactgtta tggatccagt tcatttcaac aaaaggttca aagtcgtcac
     8341 cgtgcggacc gctgtttttc tcctgagtac gaataaaggt gcggaaacca agtcgggtct
     8401 ggatgttgtt atttgcgtca ttctcaatac gtgagccatt ggcttcggtg tgcgcattat
     8461 gatcgacgcc catccagata gcctgtgcct gtggctggaa tatccacgtg tatttcgccg
     8521 cttccagacc aaaggattta ttcagggcaa agccgtaacc cgcttccagc gagacggttg
     8581 cgcctttcgc actataggat tcataggcta attcatcccc tttcaccgag tttttaaacc
     8641 agctatattg cgcccaggcg tcaatgtatg cgcctttttt actgatgtca tcggcaaacc
     8701 aggtggcata cagccctgcg caatagcctc tgacactccc tttcgaacga taatccgaca
     8761 cgctggaatg agttgagttg tagtcgcggg cataaccagc catcactccc aaacgccaac
     8821 tgtcactgtc ggtaaaacca ccggttaaca gatcggcccc taactgcgag acgtagcgat
     8881 gcgaggtcgt tctcaactgt ccgttgctgt cacgccaggc attatgcccg ccaatttgac
     8941 gtagccaaag tcggcttgaa cgctcctgtt cagttacagg atcgatgtag cgcatttcac
     9001 ccgcacggtc gttcagacgc ataacaaaaa gagtgttagc ggctgccagg ttggcgacgt
     9061 aacttccagc ttcgaggcgc aggacggggg taggcgcaac agtaggcgct gaacttcctc
     9121 cggagtttgt ttcaccgccg ctcgttgcct ggaagttagt cagataccag tttttattgg
     9181 tcgcttcggt attacctcgg gttaatgaat attcgtaggc accggcgaaa atcctcgaat
     9241 ccttaataaa ttccccattt gattccccct caacgctgat aatctctatc ccatttaacg
     9301 tgtaagctcc gctgcctcct gcattggtaa ctttaactcg ggttgttcca gaggtatttc
     9361 cattaataac gactttatcc gtagctgaat tatcatcacc caattcagtt ctgagatgca
     9421 atacgccatt attaccaacg taattttcag ttattgttaa tcttgttggt tcgacatccc
     9481 gtccatctgc ccgggaaata tagaccgtac tattatcaac cgtgaggttg ctcaccgttg
     9541 agtcagcttt aatatcccag gtactattat ctgaaagcga cagataggta tgagtgttaa
     9601 catctgttga aatattagct gaacccgtta tttttgaatt agttgcatcg acggtcaaat
     9661 gacctttaat attattgata aaaacatctg cagccgatga tacctcacta tccgtaatgg
     9721 tcacgttact cgtctcattt ttatggtaga ggatttcatt tgctgaccgt agtgagctac
     9781 cagataaaat atctaccgtt gcttcctgac catttatgga agactccagc acaattccag
     9841 acccctgatg cgcgactgca gatgtatttt tcagcgtcac cgtgccgcca ttataaattt
     9901 tagcagtcgc ggcgtcagga cccgttgtat aaatttctcc ctggttaaat gaaactgtcc
     9961 cctggcgggc agcgatggca tgagcattgt ccccatgcgt ggtgatatca acatcggtta
    10021 atgtaacgct ggcattggta taatcaagcg tgtcaacagc cttgacgaca gtgccattag
    10081 cgtacgcggc ataactttct gttccgtggg ttgtgatctc cccttttgcc atatccagtt
    10141 ttgctatggc attattttca ttacttcctg tatcggtagt actttcttca tcaagagaag
    10201 atacacacgc acgcgcgctt ccgggagatg cacacgcaag aacgccgact gcgtgattac
    10261 ccgcagtaac aataaatgta tcatctccgt cagaaaaaga gggggtaacc gaaccgtcag
    10321 agttgactct tattatggtg tcagtattca ggtttccact cgtttgactt agcaaaccaa
    10381 ttgagtaatc ccccgttatg acaatcgtgt ttttaccctg caaaataaca gcaccgccaa
    10441 attcacttac gattgcgcta gctgttaatg ctgtgcttag aggactattg tcagaaggat
    10501 ataaaccgcc cgttgttaca atgacgccat tgttaatggt taacttgcta ccctctcctg
    10561 caacggatac ccgtaacttc cattacccgt tgttataact agcgcattat caatatcttt
    10621 tacttttcca tcttttattg ccacagcatg tttatattca tcgtgctgaa taaactgttc
    10681 agcatatgca ttacccaccg ccagagcaat aagtcccaaa ctaaacccgg ggagtttttt
    10741 cagtttcata gattaaccat ccattcattg gattaacaaa cattcctggt caaataacct
    10801 atgagctcag attaccaaaa cggagggtaa agacaaaaac aaaagcacaa aaaatttaaa
    10861 tagataaaca gcgacaatat ataaatgata aataagtcgg cattatcatt tttcacctta
    10921 ttttcatgac attgatcact ttgaggtgac gattttgttt aacaaataac tcttcgacat
    10981 cacaatcatt gatagttgca ataaaaatta tttcaatgac atataaaaac aactgtgata
    11041 acaccagggt tgacaactat ttgcgcaata attcctttac ttcaatgatc caggcattca
    11101 ctgccagcaa cccattcagc cgcatactga cagtgagaaa ctgaaagatt attgctgatc
    11161 cctgtggctg acgagtcttt cccttaatcg attaaccaga ttgatcgcca gccaaacaag
    11221 ggcattaagc aaatttatcg tcacaagcac ttctcccagc ccggtactgg gttttgcaat
    11281 aaggatgacc aggagggtga tcagcataaa tgcaatggaa aagatgatat agagagtgaa
    11341 ataatccaga ttattactat tgttcatgct actaatggca atcctgtcaa aactttcaaa
    11401 caattatagc aaacaaaata atgaacaata gtaacaagag gcaacgagct aattagctgt
    11461 ctataattac accgcactat ggccttactg atcaattttc actcaaccac aaccttcacc
    11521 ttcggtttag aaaggataat tttgaccagg tcgatgtatg taacaggttt atagaaataa
    11581 taaccctgct gaaatgtgat attattttgg ttcagatagt caagttgttc tttcgtttcg
    11641 acaccttcag cgacgatact gatcgaaagt ttacgcgcca gatccaatac acaatccaga
    11701 attcgggttg attctgggtc tgcgtttacg cggccaacga aactgtgatc aattttgata
    11761 taatcaatat gcaggtcatg aagataagaa agccctgagt agccagtacc gaaatcatcc
    11821 agcgcgatga caaaaccatt ttcatgcaga atgttcaacc gctgaaccag actttcatca
    11881 acattcaatg gctcacgctc ggtgacttcc agaacaaggt ttaaatcgcg gcgggtaaaa
    11941 ctgtcacgga aatttaaaca ctcgtcgaca aacgtcggcg aaataatatg cgaagcgcta
    12001 aaattaattc caatatgaaa accttccggc aatttactcg cgatagcgtt catctgtctg
    12061 gcaacctgat tcatcaggct ttgcgtaagc ggaacgatta atccggattt ttcagcaagt
    12121 ggaataaatg ccgcgggtga tatatatcca ccgtgaggtt gtttccagcg ggctaacacc
    12181 tcaactcccc gcaatgtccc ttcccgacca tttaccacag gttggtaaaa agggacgatc
    12241 tccccattat ttattgcccg tcgcaggatc tcttcaggcg ttgcgctttt attgaaatat
    12301 ctatcaagca ggaaggcagc ggcgcaggcg attaacagaa taaatattaa aatcccaaag
    12361 cctttatcga taagtctgta gaagctaaag agtggcggcg ggttaaagat aatagtgaaa
    12421 tgatattttg tggagtgaac cttctcggta tagggataat gtccattaag cgtcatcaca
    12481 tcacccgttg gtccaataat gtcatccgcc acgcgtaata cataccttac ccctttcaag
    12541 ggtacattaa gtgccccacg aatatgctga tcgcttatcg taaccaaaat gcgcgtatct
    12601 gcaaattggt tctgatagag caggataggt aatctattaa cggtgtcgat tgctggagcc
    12661 aacagtaaat tactgtccgg gaaaacagga atacgagaca acaggacccg attcccaggc
    12721 agggatgtac accagactat tccctgtttg agaatgatga ttgtgcgcag gtgaggtttc
    12781 agagctgctt cagtgccaag ctgatattgc tcctcgaggt tgcattcctt cccggcaatg
    12841 ttaaccgctg tttgagtcgc ttgtgacgct tcttcaagta tatgattgat attgttggcg
    12901 gcatatctcg ctcccgccag gtactctgct tttgcggaat accagagctg gatgttcaaa
    12961 atagcgacac cggtaataaa caagcatatc gcaacgagga tgggtatgag tgtattgcgc
    13021 atttgcttgt cccggccaaa gggtttaata catgaaaatg ccattcattc gctcgcgctg
    13081 gcatctacag agagcatggt agagagcata agaaatatcc acgccgaaga taaaattttc
    13141 tccatcttga tctaacccat taattattga gcataatgta ggcagtacaa aataagttag
    13201 gcgggatatc aggcgtcaag aatggagagc gagaactctc cattcttgac acctgatatt
    13261 gcggacataa taagaaagca taacgcctga aatgctcact ttgcatcagc atggtgatac
    13321 agctgatgtt tattctaaaa ccttactcaa gttctaagag agcacggatt ccctgtcatt
    13381 aataatgata tccgttagtg tagtcttatg catttcaatg tactgcaata tttgcagata
    13441 gagatcagaa agaatcacac tggtttcatc ttttaaacgc aagcgtaaag cctgatcaat
    13501 aatatgacta tcaacatacc catgcgtcat gaacacctcc ctctcgagat ttttaaacaa
    13561 agcacattga aaaataaatc tctctgggat tgaattattc atgtcagcct cgtagaaata
    13621 ttaacccaat aaaattatgc tgtgtatagt ctaaagcgac agacaaaaat aagatgaatg
    13681 agttttctat aaacttatac ttaataatta gaagttacat atcatcagct gtgtatcgca
    13741 acacgatttc cagtgttttt ctaatcacat cagctttgag aatat
//
LOCUS       DAZGVQ010000131        13728 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000131 DAZGVQ010000000
VERSION     DAZGVQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.132"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            129..1907
                     /gene="ygeV"
                     /locus_tag="ACHLAG_003856"
     CDS             129..1907
                     /gene="ygeV"
                     /locus_tag="ACHLAG_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP0656998.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(1947..2426)
                     /gene="xdhC"
                     /locus_tag="ACHLAG_003857"
     CDS             complement(1947..2426)
                     /gene="xdhC"
                     /locus_tag="ACHLAG_003857"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFP0656999.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2423..3301)
                     /gene="xdhB"
                     /locus_tag="ACHLAG_003858"
     CDS             complement(2423..3301)
                     /gene="xdhB"
                     /locus_tag="ACHLAG_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFP0657000.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3312..5609)
                     /gene="xdhA"
                     /locus_tag="ACHLAG_003859"
     CDS             complement(3312..5609)
                     /gene="xdhA"
                     /locus_tag="ACHLAG_003859"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFP0657001.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            6024..6779
                     /gene="actS"
                     /locus_tag="ACHLAG_003860"
     CDS             6024..6779
                     /gene="actS"
                     /locus_tag="ACHLAG_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFP0657002.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            6858..6931
                     /locus_tag="ACHLAG_003861"
     tRNA            6858..6931
                     /locus_tag="ACHLAG_003861"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6890..6892,aa:Gly,seq:ccc)
     gene            7096..8310
                     /locus_tag="ACHLAG_003862"
     CDS             7096..8310
                     /locus_tag="ACHLAG_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013814373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0657003.1"
                     /translation="MLLSDLAIRRAKPKEKAYTLNDGNGLSLLIEPNGSKGWRLRYRF
                     AGKPKMLSLGVYPEVSLSDARTARDEAKRLIAGGVNPSEVRKAQKREQANKCGNTFEA
                     IAREWYEKRLDRWSPSYAEEMIETFEKDVFPYIGGRPIAEIKPMELLAVLSRLNDRGA
                     TEKLRKVRQRCGEVFRYAIITGRAEYNPAPDLASAFAPHKKEHYAYLKVDELPEFFRV
                     FNTYTGSQIVKLGMRLLILTGLRPGELRQAEWQEIDFDNRLWEVPKERMKMRRPHCVP
                     LSEQAIAILEQLKHMTGQYPFIFPGRIQHSKPMSEMAMNVLIRRIGYAGRVTGHGFRH
                     TMSTILHEQGYNSAWIETQLAHVDKNSIRGTYNHAQYLDGRREMLQWYADYMDSLEQG
                     GNVVHGKFGVCA"
     gene            8854..9057
                     /locus_tag="ACHLAG_003863"
     CDS             8854..9057
                     /locus_tag="ACHLAG_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0657004.1"
                     /translation="MANNNSFIRLSEVMRRTGYGRAWIYRLISLGRFPKPVKIGSRSV
                     AFIESEVDEWINQRIDASRNNAA"
     gene            10316..10606
                     /locus_tag="ACHLAG_003864"
                     /pseudo
     CDS             10316..10606
                     /locus_tag="ACHLAG_003864"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ash family protein"
     gene            10649..10831
                     /locus_tag="ACHLAG_003865"
                     /pseudo
     CDS             10649..10831
                     /locus_tag="ACHLAG_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            10824..11009
                     /locus_tag="ACHLAG_003866"
     CDS             10824..11009
                     /locus_tag="ACHLAG_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657005.1"
                     /translation="MLKTFRVFSRAVNARGYTVGISQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHSVHEIKGGQ"
     gene            11009..11212
                     /locus_tag="ACHLAG_003867"
     CDS             11009..11212
                     /locus_tag="ACHLAG_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657006.1"
                     /translation="MQEMTLAEASLQAQQVEVISRMIERMIERNHEEIDDHDLSVIAG
                     LIKQLSSNVAVWLMTEEEKRGEQ"
     gene            11270..11569
                     /locus_tag="ACHLAG_003868"
     CDS             11270..11569
                     /locus_tag="ACHLAG_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP0657007.1"
                     /translation="MASKNTGLTASSKTHPEFMRGDIYRDKYGGIVTIISTRERRITY
                     RREGYDYDCVMPLYQFSRDFSLMQAVPCGKPASNAKAEANIRKMKNMINAFRGKK"
     gene            11566..13704
                     /locus_tag="ACHLAG_003869"
                     /pseudo
     CDS             11566..13704
                     /locus_tag="ACHLAG_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625857.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
BASE COUNT         3573 a         3243 c         3570 g         3342 t
ORIGIN      
        1 tcattagaaa gtgataaaaa tcttatgttt ttatcaaagc caatttcgcc tgagttaata
       61 tcatcatgat ctgtgaacca tcaacgtctt caatgatcga cttttaagta agtctggaag
      121 gtaaagctat ggagcttgct actacgcagt cagtattgat gcaaattcaa ccgacaattc
      181 agcgttttgc cagaatgctt gccagcgttt tgcagcttga ggttgagatc gttgatgaaa
      241 acttgtgtcg cgtcgccgga actggcgcgt atgggaagtt tcttggccgc cagttgagcg
      301 gcaactcacg cctgctccgc cacgtcctgg aaacgaaaac tgaaaaagtt gtgacacagt
      361 ctcgcttcga tcccctttgc gaaggttgcg atagtaaaga aaattgccgc gaaaaagcat
      421 ttctgggtac gcctgtcatt ttacaggatc gttgtgttgg ggtgataagt ttgattgccg
      481 ttacccacga gcaacaagag catatcagtg ataatttacg cgaattttct gattatgttc
      541 gccatatatc caccattttt gtttcgaaac ttctggagga tcaggggcca ggagataaca
      601 tcagtaaaat attcgcgacc atgatcgata atatggatca gggcgtatta gttgttgatg
      661 atgaaagtcg ggttcagttt gttaatcaga ctgccttaaa aacacttggt gttgtacaaa
      721 ataatattat tgggaaacct atccgtttca gaccattaac atttgagagt aattttactc
      781 atggacatat gcagcatatt gtttcgtggg acgataaaag tgaattaatc attggtcaat
      841 tgcataacat tcagggccga caattatttt taatggcatt tcaccaatcg cataccagtt
      901 tttctgtagc aaatgcacct gatgaaccac atattgaaca attggttggc gagtgccgtg
      961 ttatgcggca attaaaacga ctcattagcc gtattgcacc cagcccatcc agcgttatgg
     1021 tggttggtga aagcggcacg ggtaaagaag tcgtcgcccg agcaatccat aagttgagcg
     1081 gaagacggaa taaacccttt attgctatca actgtgccgc gattccggag cagcttctgg
     1141 aaagcgaact gttcggttat gttaaaggcg catttactgg cgcttctgcc aacggtaaaa
     1201 cagggttgat tcaggcggcg aatacgggca cgctgtttct cgatgaaata ggtgatatgc
     1261 cattaatgtt gcaggctaaa ttactgcgcg ctattgaggc gcgtgaaatt ctgccgattg
     1321 gtgccagtag cccaatacaa gtcgacattc gcatcatttc tgcaactaat cagaatttgg
     1381 cccagttcat tgccgaaggt aaattccgcg aagatctctt ctaccgactt aatgttatcc
     1441 cgataactct gccaccgctg cgtgaacgtc aggaagatat tgaactattg gtgcattact
     1501 ttttacatct gcatacccgt cgtctgggat cggtttatcc tggcattgct cccgatgtcg
     1561 tcgaaatatt gcgtaagcat cgttggcccg gaaacctgcg cgagttaagc aatttgatgg
     1621 aatatctggt taacgtagtt ccttcaggtg aagttatcga cagcacgcta ttgccgccaa
     1681 atctgctgaa taatggcaca acggagcaaa gtgatgtaac agaggtcagt gaggcgcacc
     1741 tgtcactcga tgatgcgggc ggcacggcgc tggaggagat ggaaaagcaa atgatccgcg
     1801 aggcgctttc acgtcataac agcaagaagc aagttgctga tgaactgggc atcggcattg
     1861 ctacgctcta tcgcaagatt aagaaatatg agttgttaaa cacataacct cagtgaatcc
     1921 ggcctgaatt caggccggat atccttttac ttcgttttct cgcaatccag aactgtattt
     1981 acaatcatct gataccccgt gcagcgacaa agatttcccg ccagtccgcg acgaatttcc
     2041 gtaatggtta atggcttctc gcgtggtttc gccagcattg ccgtggtagc cataatcagg
     2101 ccaggcgtac aaaacccgca ctgcactgcg ccggatttcg cataagcctg ctgaacatga
     2161 gaaagttttc cgcctttcgc ttcaccttcc agcgtgcgga tctcttttcc ttcagcccag
     2221 gcggcaaggt ataagcaact gtctattgct gtgccgtcga ccagcaccgt acaggcacca
     2281 cattcaccca cgcagcaccc ttgtttgaca cttagcagtc cttgttcgcg gagtaattcc
     2341 gagagcggcg tgcctggtgc ggcgtgaagc tgaaaaggca tcccgttaat ggtgcattcg
     2401 atggtaattg tttcgctgtg attcattgca attttccccc cgccgcggcg acggcttcgc
     2461 taatcacttt tttggtcatc gtctggatga gatgcagacg aaactcttta ctggcccgcc
     2521 atgaagaacg cggggcgaca tcttgcagga cagattcgct gatagcttcc agcgtttgca
     2581 ggtttaatgg cgcattttgt gcagtctgtt cggcatgttg gcagcgaatc ggcgttggcg
     2641 cggcaacacc aaatgccagg cgtaattcgc tgaaattgcc gttatccagt cggcaatgtg
     2701 cggcgcagcc aatcgttgaa atatccattg cgtcgcgcat ggcatattta aaatgcgcgc
     2761 tgcccgcgtg ttctttcggc tgtggcggaa aatgaaaggc gacgaggatt tcgtcatgct
     2821 caagagacac tttgcccggc ccggtgtgaa agccattaat cgggacgaaa cgaacaccgc
     2881 gcggggagtg gatctccagt ttcgcgtcat aaattagcgt tggcgtggca gaatctgcgc
     2941 tggtggcacc gttgcaaata tttccaccgt aggtagcgac gttacggatc tgcggtccag
     3001 caatggacgt ggccgcagca cataacgccg ggagatgacg ttgagttata ggatcttcta
     3061 ttagctgggt aaatgtcgtt gcagagccga tacgtagcga gccatcttcc gccagcgtaa
     3121 ttccccgcag ctccgccaga ttatgaatat caacaatatg gcgataacgg tcattgtgat
     3181 ggtggagctg aatcagtacg tcagtgccac cggcgagcag tttggcctgc gggttgtcag
     3241 ccagcaggtt gatggcatcg gcaagggttg ctgcgcgatg gtaagaagca aaatcaaaca
     3301 tgatgttatc ctcaaatcaa tcctgccaga tggaactctt catataaccg ttttggcgtc
     3361 agcggcagtg tattgattgc aacaccggta gccatcttca ccgcgttacg aatagcagcg
     3421 gcaacaggaa ttattggtgg ctcacccagt gacttatgtc cgtatgcgga ttgcggctca
     3481 ttgatttcga cgaacgcgct ttccagttgt ggcagatccg gcatggtcgg cattttgtaa
     3541 tccagcagat tggggttacg gaccacgccg cttttagcat cgatgatcat ctcttcaaat
     3601 agcgcccagc caatgcccat tcccattccg ccgtgtacct gaccttctgc cagcagtgga
     3661 ttaagaatat gccctgaatc atgaacgttg aggatgcggt tgatggtgac tttgcacagc
     3721 gcaatatcga ccgtcagatc aacaaaggta cagccaaacg ccggtgggtt agtggtggtt
     3781 ttgatggagc tttcagcaga gagctgcccg ccgcgttcag ggtggtagaa agcgtccatc
     3841 gccaaatctt ttaacgacat taacggctct tccggtcgtt caaccagcac gatatggcct
     3901 tttatcaggg tcagattcat cgctgactga tgtagcatga ctgcggcgtg agcgatgatt
     3961 ttctctttta ataatagtgc cgcactgcgc agcgcaggcg cggcaacata gctctggcgt
     4021 gaggcaaatg cgccgggatc gaacggcgta acgtcggtat cttgagttga aataacgcga
     4081 acgtcgctga ccggaacccc cacggtttct gccaccattt gcgagaagac ggtgtcggca
     4141 ccctgaccga tttccgtcgc gccgctttgc acgttgatgg ttccatcctg attcatcaga
     4201 aggcgcgcgc ctgctatttc tacgccgaca ggccaggtgt tagaggtgta gctaaaacag
     4261 gcgacgccaa cgccgcggcg caaattgcct tgctggttct ggcattctgc acggcgtttt
     4321 tcccattcaa agattttccg gcctttttca agacactccg gcaaccctgc gctgtaaata
     4381 cgtttgcccg tgagcggatt agcatctcct tcgcgggcgg cgttgcgtaa acgaatttca
     4441 acaggatcaa tacctaacgc tgtcgcggcg tcatcaagca tagactcaac ggcaaatacg
     4501 acttgtggcg cgccataacc acgcatcgca ccagccgagg ggaggttggt atagcaggtc
     4561 tttgaactgt aagcgtaggc acaacgagga taaaggtaag cgactttatt cccccccgca
     4621 gaagcgatgg agtgcccgtg agatgcataa gcgccggtgt tagacagaac atccagacta
     4681 taacctttca atgttccgtc gcggttcacg cccatttgcc cgtcaatggt aaaagcgtgg
     4741 cgggtacggg ttgcgaggaa acactcttca cggctaaggg aaactttcac cggaatgccg
     4801 ccaagcttgc tggtcaggaa tgccgccatt ggctcttcca gtacatcctg tttattacca
     4861 aaaccgccac cgacaaacgg tttgatgact cgtacgcatg accagggaat atccagcgcc
     4921 tgaccaacca cgcggcgaac aatgtgcggg atctgggtgc tggaaacgat ggtaattcgc
     4981 gagtcatcct ccatccacgc cagcgatgtt acgctttcca tgtgacaatg ttgaataacg
     5041 ggggtctgat agtgcccctg tacctggtag tcggcggcat cgattgtttg ttggacatta
     5101 cccgtcgaca tcgtgctttg tttcagtaaa ttgccaccgt tatggattgg tgcagcgtct
     5161 tctgccagcg ccgcttctgg cgtggtgata acgggtaatt cttgccactc aatgctgacc
     5221 aattgcgccg ctttttctgc cgtgagttca tcgcgggcca cgacgatggc aacggcgtca
     5281 ccatgatgac gaacatggcg agttagcagt gcgcgatcgg cggtatcgcg cttgttttcg
     5341 tcaagtgtcc aggcatgccc agcagtagca aatggaatat caggcacatc ttcccaggta
     5401 aaaatcgcca gtacgcccgg caaacttctg gcttgttcat cattaatact tacggcataa
     5461 ccatgtgcga tagggctacg tacatatttc gcataacaca tgcccgccat aacataatcg
     5521 tcagtatatc gtgcccgccc ggtgacctta gcaatggcat cgacgcgcat gcatgattca
     5581 cccgtagcgg ttgcttcccg cgcttccatt gaaatcccct cgatttgaac atttggttct
     5641 tatggcaaag atttagtcta agcaggcaag atttacgcca gaaacgacga taagcgttag
     5701 gggaggtagg aagtgtgatg acgttaaaaa aacacagata tgatacgaaa caggactctg
     5761 aataggggca gtgatgcgta tcaatatgag tggcgataaa atcaatgtga taaattggga
     5821 ttattacgca tcaggatggg aaatagggta aaaagtcagg aagttcgata cctctacagc
     5881 gaatatttag ctgatgatac ttccctttgc caaatgaaat taacctcttt ctctattacc
     5941 acgtttttcc agaagcaaga gattgggtat caagaggctg gctgctatga taaggcgtct
     6001 tgttttttaa gcgaggaaag attttgagtg cgggacgcct gaataaaaaa tctctgggta
     6061 tcgtgatgtt gttatcggtt ggactgcttt tggcgggctg ttcgggtagc aaatcatccg
     6121 atacaggaac gtattccggc tccgtttaca ccgtgaaacg gggggatacg ctatatcgta
     6181 tttcgcgcac cacgggaacc agcgtaaaag aactggcgcg actgaacggc atttcccccc
     6241 cttacaccat tgaagttggt cagaaactaa aactgggtgg ggcgaaaagt agcagtagta
     6301 cacgtaaatc aaccgccaaa tcaacgacca aaaccgcatc ggttacaccg tcatcagcgg
     6361 taccgaaatc ttcctggccg ccagtagggc aacgttgttg gttatggcca acgacaggga
     6421 aagttatcat gccgtattcg acagcagatg gcggcaataa agggattgat atctcagctc
     6481 cacggggtac acctatttac gccgcgggtg caggaaaggt ggtgtatgtg ggcaaccagc
     6541 tgcgtggcta cggtaatctc atcatgatta aacacagtga agattacatt acggcttacg
     6601 cccataatga cacgatgcta gtaaataatg ggcaaagcgt gaaggctggg caaaaaatcg
     6661 ccaccatggg gagcacggat gcggcatctg ttcgcctgca tttccagatt cgttaccgtg
     6721 caacggcaat tgatccgcta cgttacttgc cgccgcaggg cagcaagcca aaatgctgat
     6781 ggcgaattaa tcagcagtca gcagcgcggc acttgctaag gagagcgtaa ggtttataat
     6841 gccttacgca tctcgaagcg ggcgtagttc aatggtagaa cgagagcttc ccaagctcta
     6901 tacgagggtt cgattccctt cgcccgctcc aaacaaacat ctaccaacgt ctactaaagt
     6961 ctaccaatcc cccgaatatc aaggcctcca gcgattttcc tcgtctacaa ccgtctacag
     7021 ttatctacct aaatctacac acagaagtgt atagtaatgc gtataggaca aaatcgtttt
     7081 ttgggggcta tacacatgtt gctttcagac cttgctatcc gtcgcgcgaa gccaaaagag
     7141 aaagcctata cactcaatga cggcaacggg ctttcgttac tcattgagcc gaacggttcg
     7201 aagggctggc gattgcgata caggtttgca ggcaagccca aaatgttgtc gctgggtgtt
     7261 tatcctgagg tgtccctttc tgatgccagg acggcaaggg atgaagccaa aaggcttatc
     7321 gctggtggag ttaatccgag tgaggtcaga aaggcccaaa agcgagaaca ggcgaacaaa
     7381 tgcggcaaca cctttgaggc gatcgcccgt gagtggtatg aaaaacgcct ggaccgatgg
     7441 tctccctcat atgcagagga aatgatagag acgtttgaaa aagacgtttt tccgtacatt
     7501 ggaggtaggc ccatcgcaga gattaagccg atggaattgc tcgcggttct atcccgtctt
     7561 aatgaccgtg gcgcaactga aaaattgcgt aaggttcgcc agcgatgtgg cgaagtgttc
     7621 cggtacgcaa taattaccgg acgcgctgaa tataaccccg ctcctgatct ggcaagcgca
     7681 tttgcaccac acaaaaaaga gcattacgcg tatctgaaag ttgatgagct accggaattt
     7741 ttccgcgttt ttaatacgta caccggaagc cagatcgtca agctgggtat gcgattgttg
     7801 attcttactg gacttcgccc aggtgaatta cggcaggcag agtggcagga gattgatttt
     7861 gataatcgtc tgtgggaagt gccgaaagaa cgaatgaaaa tgcgccgtcc tcattgcgtg
     7921 ccactttcag aacaggcgat cgctattctg gaacagttaa aacacatgac ggggcagtat
     7981 ccgtttattt tccctggtcg aatacagcac agtaagccga tgagtgaaat ggctatgaac
     8041 gtgcttattc gtcgtattgg ctatgcaggg cgcgttactg gtcatgggtt ccggcatacc
     8101 atgagtacga ttttgcatga gcagggttat aactccgcgt ggatagaaac gcagctggcg
     8161 cacgtcgata aaaactcgat ccgtggcaca tacaatcacg cgcaatacct ggacggacgg
     8221 cgggagatgc tccagtggta cgccgactat atggactcac tggaacaggg cggtaacgtg
     8281 gtgcacggca agtttggtgt atgtgcataa aaacgactgg acaagtatac agatgtatac
     8341 taacgtacac ggaaagctac aggtaatatc cttacagatg gtttttcacg cagttacccc
     8401 atcatgtatg gacggcgctc cttgtaacaa cgaagatgga gtcttctacg cagctgaaaa
     8461 gctggatgct ctttaacagc ctttggtgct ttcctgatgc aggaaagaat agggatacat
     8521 gcacctttac aatgcatgta atgattcacc aatgaggctg cggagcgctg acaaagcgca
     8581 attgctctct tgcctgccca ccatgccctg aaaaagggcg ctctttaaaa ctactggacg
     8641 tcacgcctaa tcgtgtgacc ccgccgaaaa gcgggaatag ctctaccttg ctggagcata
     8701 ccaaccacca accaaacaat ctgaatttat cttatgcaat accacaacat gttttcatgt
     8761 tgtggttttt gtcgtgttcg taatttgacg caaataaaac tcttgctaag ccacaggagg
     8821 ggattttcgc gtcaacatta aggagattga taaatggcta ataacaattc attcatccgt
     8881 ctttctgagg ttatgcgccg tactggttac ggtcgcgcat ggatttatcg cctgatcagc
     8941 ctggggcgtt tccctaaacc ggtaaaaatt ggttcccgct ccgtggcttt tattgaaagt
     9001 gaagtcgacg aatggattaa ccagcgtatt gatgcttctc gcaacaacgc cgcgtaataa
     9061 tcttttggga gggggatatt ctcccccagc actaaacctg attcgatagc aggagttttc
     9121 aatgaaacgt ccgaatgtgc ccgtacaggg gcagggcttc gttcagcctg aaatcagcca
     9181 gaacgatata ttgattcgcc acaaagcaaa gtcgcgtatt gattcccgcc tgtttgctga
     9241 acgcatcggc ataaagcaca aaagccttta ttcgcttatt cagaagcaca aaaccggatt
     9301 gcgtgaactg ggtatccttc cgtttcaaac ggagcgatta aaaaccaatc aacacggaga
     9361 gcgtgaacat aagtttgctc tgctgaatag cgatcagttt gattacatgc gccatatcgt
     9421 gagccatttc gccgctgagc gtatgaaaca cgttaagcaa tacttagagg aggttttatc
     9481 aaattatcgc gctggtggct ctgtacggcg cgaatatccg catgatgcag gaatggcagg
     9541 caaaacaaag gcgaccacga aagggtcgcc agtggagata aaggaaatca agcgcatcac
     9601 caactatgcc atgagtgtag ctggtggaca aggctatcag ccaggcggtc ggtttttagt
     9661 ggcttttaac cgccgtgggt atttctcgac aaaatcttca agggcaaatt ttttagcttt
     9721 tgtatgtgat gcctttgact cgtcatcaca tgtttttccg cgccttatcc tgttttcaat
     9781 aaaacgggag gcggcctttt tattcagcga tgaatcaatg gggctgctcc ctgtttgctg
     9841 ctcgacaatg gcatccatga gtagttggtg ttgtagttgt cgtaaaaatt tctcatcttc
     9901 tggtggtaaa tccatactta tttcctgtac agcggagcga cgcagcacct cacaccacgg
     9961 cgctgatggt tgttattcgt tttatggagt cagagaagca aaagcggatc gctatcggtt
    10021 tttaggcgag attaatcgcc gtgggtattt gtccagaaat gcctcaagcg caaatcgttt
    10081 tggtctggtg agaggagagg gattaagcaa agactcgcgt tccaggcgta ctttttcgcg
    10141 ctggcgctca agtatggata atgcgatttc ctgtaattct tcatttgtca ttgtctgtac
    10201 ccgtaaagcg gtgcgctgta gtacctcata tcacagcgca ggtatttttt atggttgtaa
    10261 tttgcgtgat gattcaggtc gctttgcgac atactgcata aacataaata acgtgatgtt
    10321 attatgtgct ttcaaagaaa acgcaggtat ttttcgcaca aacttctctc gcaacacatc
    10381 cgaccactta agcaaaaaag cggcatatac tgaggctatc ccttataaaa cggggatcgg
    10441 gattaccacc ccgcaggagt tagaggcgat acacgacgcg ccagcgtctt tttttgtatc
    10501 ggcgcacacg cacacctcaa caatggtggg ctgtatgggg ccgacttcgg tcgggctggt
    10561 ttcctctaac gccagttgtg gtaaccctgt acagtccacc gccagcgagc ttaccacctc
    10621 cggcggtggg tataatccca aagtcagagg agcctttcac atggctgtat ccgcacgccc
    10681 ttactttgtc tggcgcttta tgcagtgcct gacaggcagc attgcaatat tcaccgttac
    10741 cgctgccact gagcgcgaag cacgcgccca gctgccgcac gcacatctga tttttgtcgc
    10801 ccgtatccgt caggaggtga ctcatgctta aaaccttccg tgtcttttcc cgtgccgtta
    10861 atgcacgcgg ctataccgtt ggtatctcgc agaacgtgaa ggccgaaaac gtccaagctg
    10921 caatcagcac agtaattaac aaatcagcta aagccggttt atccggcgta attatccact
    10981 ccgttcatga aattaagggg gggcagtaat gcaggaaatg acactggcag aagcctcgct
    11041 gcaagcgcaa caggtcgaag taatcagccg catgattgaa cgcatgattg aacgcaacca
    11101 cgaagaaata gacgaccatg acctttctgt tattgccgga cttatcaaac aactttccag
    11161 caatgtggcg gtgtggttaa tgacggagga agaaaaacgc ggcgaacaat aacggctaaa
    11221 aataaacggg aaataaagat taagacgact ccttcaggag attaattcaa tggcatcaaa
    11281 aaatactggc ttaactgcca gtagcaaaac tcaccccgaa ttcatgcgcg gggatattta
    11341 tcgcgataag tacggcggca tcgtgacgat tatcagcacg agagaacgac gtatcaccta
    11401 ccgccgtgaa gggtatgact atgactgcgt gatgcccctg tatcagttca gtcgtgattt
    11461 ttccctgatg caggccgtgc catgtggtaa gccagccagc aacgcaaaag cagaggcgaa
    11521 catccgaaag atgaaaaaca tgattaacgc attcaggggc aagaaatgaa aagcgcaccg
    11581 aatttaaaaa aacagccgaa agacaaaatg accgaagtca ttatctttgc gggtagtgat
    11641 gcctgggcac atgcgaaaga gtggagcgaa tgggccggaa agcatattgc agcagatgat
    11701 acaccgcccg ttattcttgg accgaaacaa ctggcgagcc tggaagacac gaaaatcatc
    11761 gataaagggc gcaattacgt gcgcgtttat cgtgctggtg aaatatcaga gacagcactt
    11821 ttacagatag caacactgct tgcggtggcg ggggtaaagg aggcacgttg ctacagcggc
    11881 tttgttgacc agcagccgga ggactggaca ccgcgcatgg ctggactgaa ggatgatgcg
    11941 gaacgcggca actgcctggt gatcaatctt ccggcaaaga caaatttcac cggaaattat
    12001 gatgacgaat taaaaccccg cgttgaatgc cgtcctgatg gcgtttactg ggtgacgcca
    12061 aaggttgata agcaatctgg cgaaattatc cgtccgttcg aatggctgtg ttcttccgtt
    12121 gaattactcg gttcaggtgt cattggtaac gagcattacc gcgtgatgcg ctggacagat
    12181 tcgacaacaa atcggcttgt cactatggct gtgccttgct gcggaatagg cgacactgac
    12241 ggctggcgat tactgaaagc caacgggctt aaagtcacga caaacggaaa acacaggatc
    12301 cccctggctg actggatgca gttaggcgga cagcatgagg aatggcactt aagcacgaaa
    12361 gctggctggc actttggcgc gtacatcatg ccggacggta cggttatcgg cgaatctgac
    12421 aagcccgttt tgtttaccgg aaaaagtgcc gcgattaacg gttattccgt ggcaggaacg
    12481 gcggaaagct ggcgcgacag tgtggcgcgg ctggctggtg gcaatccgtt catgatgctg
    12541 ggtatagcga cgtcattatc cgcgccatta gtcgggctga caggcgctga tggttttggc
    12601 gtgcattttt tcgagcaatc cacagcaggc aagaccacaa cgcagagcat cgcgtcgagc
    12661 ttgtggggcg atcctgaggc gctgtgatta acctggtacg gaacggcgct ggggatagct
    12721 aacgaagcag aagcacacaa tcacggactc cttccactgg acgagatagg gcaggcagga
    12781 agcccccgtg acgtgtacgt atcctcatac actttgttca atggattcgg aaaattacag
    12841 ggggcaaagg acggcggcaa cagagagctg aaaaactggc ggaccgttgc tatcagcacc
    12901 ggagaggtgg atatcgaaac atacctcaaa acgtcgggga taaaagttaa ggtcgggcaa
    12961 cttgtgcgcc tgctcaatat cccgattcaa aaagccacgc agttacacga atacagcaac
    13021 ggaaaggatc acgctgacgc attgaaggcg gcatggcaag aaaaccacgg cgcagcgggg
    13081 cgtgagtgga ttaaatggct tgcggagaac cagcaggaag ctaaggacgc ggttagagag
    13141 agccgtgcac gctggcgaaa tatcattcca gacaattacg gtgagcaggt ccaccgcgtg
    13201 ggtgaacgtt tctccatact ggaggccgcg cttgtgcttt ccggtcatgt aactggctgg
    13261 tccgtgcagg aatgccgcga tgcaattcag tatgtattta atgcctgggt aaaagaattt
    13321 ggcacgggca acaaggaaca caaacagatc attgagcagg cagaggcgtt tcttgctgcc
    13381 tacggcatga gccgttttgc gcccgttgat tatgacccag ccagcctgcc gatttctgag
    13441 ctgtacggct acagggattc gggaagccgt tacgatgaac cattgttgtt ttacgtatta
    13501 tcggagccgt ttaaatcgaa agtagcggaa ggcttcaaca aggataccgt tgcaaaaata
    13561 ctccacgaag ctggaatgct gaaacgacca gagagcggga gaggatggaa aatcaagacc
    13621 ccacgcctta agaaccttaa tggagcgcaa ttatgggcct acgggatgtt gttcgctaat
    13681 cacgatgagg agggaagcga ttaactacgc gtatataaga tgaaaaaa
//
LOCUS       DAZGVQ010000132        13694 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000132 DAZGVQ010000000
VERSION     DAZGVQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.133"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(163..375)
                     /gene="cspH"
                     /locus_tag="ACHLAG_003870"
     CDS             complement(163..375)
                     /gene="cspH"
                     /locus_tag="ACHLAG_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFP0657008.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            661..873
                     /gene="cspG"
                     /locus_tag="ACHLAG_003871"
     CDS             661..873
                     /gene="cspG"
                     /locus_tag="ACHLAG_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFP0657009.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            884..1072
                     /gene="ymcF"
                     /locus_tag="ACHLAG_003872"
     CDS             884..1072
                     /gene="ymcF"
                     /locus_tag="ACHLAG_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP0657010.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            1047..1277
                     /gene="ymcE"
                     /locus_tag="ACHLAG_003873"
     CDS             1047..1277
                     /gene="ymcE"
                     /locus_tag="ACHLAG_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFP0657011.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            1267..1440
                     /gene="gnsA"
                     /locus_tag="ACHLAG_003874"
     CDS             1267..1440
                     /gene="gnsA"
                     /locus_tag="ACHLAG_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFP0657012.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1488..2561)
                     /gene="yccM"
                     /locus_tag="ACHLAG_003875"
     CDS             complement(1488..2561)
                     /gene="yccM"
                     /locus_tag="ACHLAG_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP0657013.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(2633..5377)
                     /gene="torS"
                     /locus_tag="ACHLAG_003876"
     CDS             complement(2633..5377)
                     /gene="torS"
                     /locus_tag="ACHLAG_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFP0657014.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYF
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAKLEQQPLSAPLPHEEITRSVADLEAWLNKKDLNAI
                     "
     gene            5460..6488
                     /gene="torT"
                     /locus_tag="ACHLAG_003877"
     CDS             5460..6488
                     /gene="torT"
                     /locus_tag="ACHLAG_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFP0657015.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6461..7153)
                     /gene="torR"
                     /locus_tag="ACHLAG_003878"
     CDS             complement(6461..7153)
                     /gene="torR"
                     /locus_tag="ACHLAG_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFP0657016.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            7283..8455
                     /gene="torC"
                     /locus_tag="ACHLAG_003879"
     CDS             7283..8455
                     /gene="torC"
                     /locus_tag="ACHLAG_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFP0657017.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            8455..11001
                     /gene="torA"
                     /locus_tag="ACHLAG_003880"
     CDS             8455..11001
                     /gene="torA"
                     /locus_tag="ACHLAG_003880"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFP0657018.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAESIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            10998..11597
                     /gene="torD"
                     /locus_tag="ACHLAG_003881"
     CDS             10998..11597
                     /gene="torD"
                     /locus_tag="ACHLAG_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFP0657019.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVVRCRQYDSFGFYAALSQLLLVLVESDHQNR"
     gene            complement(11690..11995)
                     /gene="cbpM"
                     /locus_tag="ACHLAG_003882"
     CDS             complement(11690..11995)
                     /gene="cbpM"
                     /locus_tag="ACHLAG_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFP0657020.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11995..12915)
                     /gene="cbpA"
                     /locus_tag="ACHLAG_003883"
     CDS             complement(11995..12915)
                     /gene="cbpA"
                     /locus_tag="ACHLAG_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFP0657021.1"
                     /translation="MELKNYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            13176..>13694
                     /locus_tag="ACHLAG_003884"
     CDS             13176..>13694
                     /locus_tag="ACHLAG_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657022.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQNVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENITACDDVFSEYCFD
                     DENMSIQPEQINT"
BASE COUNT         3401 a         3395 c         3540 g         3358 t
ORIGIN      
        1 actataactg gctgttaaat ggcatttata actattaggt gcagagatat tcgcttaaat
       61 ggattagttt ctttgtggaa aagtaactga tgttataatg tatgatgttg actattgcac
      121 tattccaata aaaccagtca gctttaaaca agcagcgtca tattaagaga gataaacatt
      181 tgccgctgtt ggtcctcgca ggccatttac gcggcaaaat tccacacgta atcctggtat
      241 aagcacttct gcgtcgcggg gagtgaatgc ggaaatatgg acctgaactt ctttacgacc
      301 gtcggagggg ataatgaatc ctttgccgct tttgcgatca aaggttttga caattcctgt
      361 cattttacgg gacaaacaaa ttccttactg aaaatactgc gctgcactat acggggttaa
      421 taaaataaag ccagcgatat ttaagaccgc cggacggcta aaataaaatt tgcttaatct
      481 caattatcat gcgttaatag ctgcgtcggt ttgaaagaca gacagcatac aaagtagttt
      541 actaaagcag ttctcattat caggcattat ccccttcttt tgagtctctc tcctgaacac
      601 taagtagttt ctgtattaaa gccctgtttg ccgaaaggcc caaaatgaag gaagtaaaat
      661 atgtctaata aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt
      721 atcactcctg atgatggcag caaagacgtt ttcgtccatt tcaccgccat ccagagcaat
      781 gaattccgca cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt
      841 ggccccgcgg cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca
      901 tttccgctgt ccatgttgtc atggttcaca gtaccgcaca tcggcattcg atgtgacgga
      961 gcgaaaccct ttgggcgcta agtgtatttt ttgtaaatcg acgatgatca cctttgataa
     1021 cgtcgcgctg caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatattaggc
     1081 tccctcgtgg agcctttttt atatctgcct tatttttctt caacgctgta tgtatagtaa
     1141 gcgataacct gttgattatt gaatctttcg gggagatggc ttataacatt tcttacctga
     1201 ccagggtacc gggaaccaac accttactgg cgtgttgctg tcttttaaga ccagaagagg
     1261 ttaacagtga atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc
     1321 gcgcaaaaaa tcgccgagct gaacaagaat acagggaaag aagtctctga aattcgcttc
     1381 accgcacgag aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga
     1441 ttttgtgaac atcacccgtg cgaggtgatg ttccgcttgt tgctaattta gtgaccaatc
     1501 attggcgctt gtggaattaa gcgtcggtac aattcatccg gtaccgggct ttgccatact
     1561 cccgcataca ttgcgtaacc aatcaccgca aacataatcc ccagaaccag tagcgtcatt
     1621 aaccagccag acaacgcaaa ggctttttta tttgccgcag gtttttgcag tgaaaaggtc
     1681 aatgttgagg ctaccggaca tgactctacg caagtcatac agccggtaca ttccactgtt
     1741 cgtacctgaa ttaatttatc gaccgggatc cgtgatgggc aattttttgc gcatttgcca
     1801 cagtcgatac aactttcggc attgcgacga atcttaaacg gcgacaatag cgaaaccacg
     1861 cccatcagcg cgccatatgg gcaaagataa cgacaccagg catggcgaat aaacaggctg
     1921 gcaatcagca aaacggtcac gctgattaat gtcgcggtcc ccatatgacg aaagaaatcg
     1981 agcattttaa cgtccatcac cacgctgtag ggcgacaaca taaaatagtg aatcgcctga
     2041 gcgggcatca ataatgcgat atagagaaaa aaactcaaca gcaaatactt cacgccgcgc
     2101 agaggaatat ccagccagcg gggaaggaca cattgccgac caaacagttt gttaccgaga
     2161 tcgccgatta attcagaaag cgtaccaacc gggcataacc atgagcaaaa ggcctttttg
     2221 agtaatagac tgatgacgat aaaagcgacc aataacagca tcgcggcggc gtggacggac
     2281 ggtaactgac ctgttgtaag gctatatttc agattcatca gaccggcaat cggtagccag
     2341 ccttcgatac ctcccggtct ggcgacaaat gtcgtgctac ttgccgtttc gtaatagcgc
     2401 acccaatacc agaacgtgat ggcaatataa atattcattg ccaacagtaa taattgcgtc
     2461 gctttacgcc aggtcgtggc attgcgccag tcattccacg gtaatttgcc gcccgtcgtg
     2521 cctggccgcc gctgccagcg ggttcttttt ttctctgcca tgattttgcc agtccgttaa
     2581 gttgcatacc aaatgccact attctagttg ttcttaactg gctgatattg attcaaatcg
     2641 cgttcaggtc tttcttattc aaccatgctt ccagatcggc aacactgcgt gtaatttctt
     2701 cgtgtggaag gggggcagat aatggctgct gctccagttt tgcgcatagc tggctggcga
     2761 tacgcattcc caaactcgag caactgcttt ttagctgatg cgcggcacgc tttattttct
     2821 cgctatcctg actggcgcgg gcaatgtcga tttcatcgag aagcggcagg gcatgttgtg
     2881 taaataatac cagccattcg tggatcttct ccgtccccat taactgagca tcttcattga
     2941 gttgcgatac atccagcgat tgatcgttat tgacttgcag ttggaaatag tgcgccagta
     3001 actgaccgag cacttcacgc ggcaccggtt tagggataat cccgcggaat aatgaactgg
     3061 tacgctggcg cagcgtttcg tcaatgacat gggcgctaaa gccaatcaaa accagcgacg
     3121 gatattgctg tgccagttgt cgggcaagcg taatgccgtc gacatccggc agatcaaaat
     3181 ccaccagtgc ggcagcaaac ggttcgctat tttgcagtgt ctctaaagcc tgcgcggcat
     3241 tgccaacagc aacaacctgc gcaccactgg tgttcagcat ctcaacggta attcgctggg
     3301 ttagcgggtt atcttcaatt aacagcaaac gtaaaccgtc aagacgcacc gcctgattga
     3361 ctgtttttgg cacgggtgcc gtggcaacac gtaacggcaa gcgtaaacaa aaacagcttc
     3421 caacctccgg cgtgctggtg gcgctcagtt cgccgcccat cgcctgggcc agacggctac
     3481 tgatagtcag ccccagcccg gtgccgccgc gtttgccgct tacctgcaca aatggctgga
     3541 agatttctgc cagtttcgcg ggatcaatac cgcagccgct gtcttccact tcgaccagcc
     3601 attgctcgcc atcagtgcga ctacgcagga taatgtaccc ttcgtcagta aaacgcaggg
     3661 cgttgctcaa caggttggtt ataacctgac gaatacgtcg tggatcgccc attaacgcgc
     3721 acggcatatc atcggcaatt gccgttgcca ggcgaatcgg gcgacctttc acccgtccgc
     3781 tcattaattg cagggtactt tccagcagcg ggcgcggttc aaagggctca tcgctgaccg
     3841 aaacattctt gccacctgct tcgatagcgg aataatcgag aatatcgttg aggatggtca
     3901 gcaacgattc accagagtca gtaattgccc gcaaatcatc acgctgggcg ttaagtgcgg
     3961 ggttatctgc cagcagttga gcagtgccga gaataccgta cagcggtgtg cggatctcat
     4021 ggctcatcgc cgccagaaac gccgattttg cctggctggc tttttctgct tccgcccgtg
     4081 cctgtcggtg ttctatcacc agttcctgca attcagctgt acgcgctttg acctgcgccg
     4141 ccagctgttc gcggtggcga ttcagtgcat gaacattgct gcgaaacgca tccatcagcc
     4201 gcccgatggt atccagctcc cgtacgccag cggtttccgg gaaaggggag tcaatatcac
     4261 cgtccagcag ccgttgcagc gcctgcgttt gttcggcaag tggacgcgtg actgagcgat
     4321 aaaccacgcg ccaaaggatc agaatcagtg cgcaaagtga aaccatcccc agcaataaca
     4381 ggctgtattg cccgcgtgca ctggcttttt ccagatgcgc cagtccgtgc tgattacgca
     4441 gctcaatggt gtcgaccagc tgactgactt cgctactaaa ctgcgcgaac tgggcgatgt
     4501 tattttgtgc gagagtttgt aggtgattgc tgatttcact gtcctgctga tacagcgcca
     4561 gcaaatcgct atattggcta acggtagtta acgttgttgc gacctgcgca cgaacacccg
     4621 gatcttcaat gcgtatttgc cgacgttgca gaattttcac cgcattattg agctgctttt
     4681 ccagcgttgg tgcatttttc tggatctgct ccagccccag attcatcacc atttgctgca
     4741 cccgcagagc gctaaggcgc agttcattca tctggttaac atactcaaga tcgatatcaa
     4801 tcagccgatc gagtgcactt tcagcagcct gacgctgatc ttgttcgatc aaatcgtaaa
     4861 tcccggcctg ggtcgctcca gcggaagttg tcgcattatt cgcctgacct tgcgccaggc
     4921 gtgcgatctc atcggcggca gcgactatct gctgactgag ttgccgttgt tgctggcgta
     4981 gttgcagacg ccgccccacc agttcccctt gctgacgtaa tgaacgggag atctcctgct
     5041 cctgttgttc aatagcggtg gtatcaaaac cttgttcccg taacgcttgc agcaacgcat
     5101 taatcttcag gctttgtgcg gtgagcattc gcccctgcgc ctgccacatc ttttcgttat
     5161 cggcactggt caggttctgc gcggcgaaaa gttcccaggc gctggcttcg ctcaactggc
     5221 gcgccatatt catggtagga atcaatgcct gagtgttgtc tttttccacc tggctgataa
     5281 agcgcaggtt gtaccatccc accagggtac tggtcagggt taacagcgcc atcagggcaa
     5341 agcccatcca gagtcttcgg gtcagggtta aattcacggt cggtgcactt aaggtgaaaa
     5401 aggttgagtc gcaaagcgga atgcatctag cataaagcct tattattgat gaggctatca
     5461 tgcgcgtact gctattttta cttctttccc ttttcatgtt atcggcattt tcggctgata
     5521 acctgttgcg ctggcatgat gcgcagcatt acacggtgca agcctctacg ccgcttaaag
     5581 ccaaacgcgc atggaaactg tgcgcgcttt atcccagcct gaaagattca tactggttat
     5641 cgttgaacta tggtatgcag gaggcagctc gccgctatgg tgtggattta aaagtgctgg
     5701 aggcaggcgg ctacagccag ttggctaccc agcaagcaca aatcgaccag tgtaaacagt
     5761 ggggcgcaga ggccattttg ctcggtagta gcactacgtc atttcccgac ctgcaaaagc
     5821 aggtagcaaa tctgccggtg atcgaactgg taaatgctct tgatgatccc ctggtgaaaa
     5881 gccgcgttgg tgtaccctgg tttcagatgg gctatcaacc tgggcgatat ttggtgcaat
     5941 gggcgcacgg taaaccactg aacgtgctgt tgatgcccgg acccgataat gccgggggca
     6001 gtaaagagat ggtcgagggt tttcgcgcag ccattgccgg aagcccggtg cgtattgttg
     6061 atattgcgct tggtgataac gatattgaaa tccagcgtaa cctgttgcag gagatgctgg
     6121 aacgccaccc agaaatcgac gtcgttgccg gaacggccat tgcggcagag gcggcaatgg
     6181 gtgaagggcg taacctgaaa acgccgctta ccgtggtgtc gttttatctt tcacatcagg
     6241 tgtatcgcgg gctgaagcgg ggaagagtga ttatggctgc cagcgatcaa atggtctggc
     6301 agggggaact ggcggttgag caggccatca ggcaattaca ggggcaatcg gtgtctgata
     6361 atgtcagccc accgatttta gttctgacgc cgaaaaatgc cgaccgtgaa catattcgcc
     6421 gctcgctgtc accaggggga tttcgtccgg tctattttta tcagcacaca tcagcggcta
     6481 agaaataacc ttcaccatgt tgcgtcacca gtaaatccgc gctgagttta tgacgtaaac
     6541 gacgaattaa cacatcgacg gtgcgcaggt cagggttttc cacccgacgc gcagaaagca
     6601 tacgtagcag acgttcacgg ctgagaattt cgcccggatt cgtcacaaat gccaccaaca
     6661 tttcatactc tgcgcgggtc agtttaatcg gctcgccatc ccgctccagc gtatggcgcg
     6721 acacattcag gcaataaccg gcaaagcgat agcagttgtc ctgagtgtgc ggttgagctt
     6781 gtcgcgcgag gtcgattcgc cagagcagat ttttcacccg tactaccagt tcgcgcagtt
     6841 ccagcggttt ggtgacgtaa tcgtctgcgc ccatttccag cccaacaata cggtcaatcc
     6901 gatcgctgcg tccggtaacc agaataatcc ccaccgttga gcgttctcgc agggcgcggg
     6961 ttaacatcag gccattttca tcgggtaagt tgatatccag cagaattaaa tctaccggct
     7021 gattctgcat aatttcccgc agcccggcac cgctcgctgt aacggaaacg gtatacccct
     7081 cctgagtgaa gtaagattgt aatcgcgcct gggtaaccgg ctcatcttca acaataacaa
     7141 tgtgatgtgg catcagaggg ttttactcat tctgttcata tctgttcata ttctgccgta
     7201 agccgttcat cctgaccagt gccgctgttc atatttgctc attaagatcg cttcactaaa
     7261 ccataatttt acaggggcta ttatgcggaa actctggaac gcgctacgcc gacccagtgc
     7321 tcgttggtcg gtactggcgc tggtcgcaat tgggattgtg attggcattg cgctgattgt
     7381 attgccacac gttgggatca aagtcaccag cacaaccgaa ttttgtgtca gttgccacag
     7441 tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat ttccagaacg cctccggcgt
     7501 gcgagctgaa tgccatgact gtcatatccc gccggatatt ccaggcatgg tgaagcgcaa
     7561 actggaagcg agcaatgata tctaccagac ctttattgct cactccattg atacacctga
     7621 aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa tgggcgcgaa tgaaagaaaa
     7681 caactcggca acctgccgct cctgccataa ctacgatgcg atggatcatg cgaagcagca
     7741 tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat aatcaatcct gcatcgactg
     7801 tcataaaggt attgcccacc agttaccgga tatgagtagc ggcttccgta agcagttcga
     7861 tgagctgcgc gccagtgcta atgacagtgg tgacacgctg tactctattg atattaagcc
     7921 aatttatgcg gcgaaaggcg ataaagaagc ctctggttct ctgctgcctg cttcggaagt
     7981 gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa attaccggct ggacggaaag
     8041 cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa cgcatctttg ttgcctcgat
     8101 tcgtggtgat gtgcagcagc aggtaaaaac gctggagaaa accaccgttg ccgacaccaa
     8161 taccgagtgg agcaagttgc aggccactgc gtggatgaag aaaggcgaca tggtgaacga
     8221 tatcaaaccg atctgggctt atgcggattc gttgtacaac ggcacctgta accagtgcca
     8281 cggcgcaccg gaaatcgccc actttgacgc taacggttgg atcggcacgc tcaacggcat
     8341 gattggcttt accagtctcg ataaacgtga agaacgcacc ttgttgaaat atctgcaaat
     8401 gaatgcgtct gacaccgcag gtaaggctca cggtgataag aaggaagaaa aataatgaac
     8461 aataacgatc tctttcaggc atcacgtcgg cgttttctgg cacaactcgg cggcttaacc
     8521 gtcgccggga tgctggggcc gtcattgtta acgccgcgcc gtgcgactgc ggcgcaagcg
     8581 gcgactgacg ctgtcatctc gaaagagggc attcttaccg ggtcgcactg gggggctatc
     8641 cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac cgttcgaact ggataaatat
     8701 ccgtcgaaaa tgattgccgg attgccggat cacgtacaca acgcggcgcg tattcgttat
     8761 ccgatggtac gcgtggactg gctgcgtaag cgccatctca gcgatacctc ccagcgcggt
     8821 gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg acatgttcta tgaagaactg
     8881 gaacgcgtgc agaaaactca cgggccgagt gccttgctga ccgccagtgg ttggcaatca
     8941 acggggatgt tccataacgc ttcggggatg ctggcgaaag ctattgcctt gcatggtaat
     9001 agcgttggta cgggcggaga ttactctacc ggtgctgcgc aggtgatcct gccgcgcgtg
     9061 gttggctcaa tggaagtgta tgaacagcaa acctcctggc cgctggtatt gcagaacagc
     9121 aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc agcaagcgaa ctggtggtgc
     9181 ccggatcacg atgtttatga atattacgcg cagctaaaag cgaaagtcgc cgccggtgaa
     9241 attgaggtca tcagcatcga tccggttgtc acatccaccc atgagtatct ggggcgcgag
     9301 catgtgaagc acattgcggt taacccgcaa actgatgtgc cgctgcaact ggcgctggcg
     9361 tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc tcgctaacta ctgtgtgggt
     9421 tttgagcagt tcctgccgta cctgctgggt gagaaagacg gtcagccgaa agatgccgca
     9481 tgggctgaaa aactgaccgg cattgatgcc gaaagcattc gtgggctggc gcggcagatg
     9541 gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc agcgtatgca gcacggtgaa
     9601 cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg ggcaaattgg cctgccaggt
     9661 ggtggctttg gttttggctg gcactataac ggcgcaggca cgccggggcg taaaggcgtt
     9721 attctgagtg gtttctccgg ctctacgtcg attccgcctg ttcacgacaa cagtgattac
     9781 aaaggctaca gcagcaccat tccgattgcc cgttttatcg atgcgatcct cgaaccgggg
     9841 aaagtgatca actggaacgg taaatcggta aaactgccgc cgctgaaaat gtgtattttt
     9901 gccggaacta acccattcca tcgccatcag cagatcaacc gcattattga aggctggcgc
     9961 aagctggaaa cggttatcgc catagataac cagtggacct caacctgccg ctttgccgat
    10021 atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc tcgaccagta cggcaaccac
    10081 tccaaccgtg gcattatcgc catgaaacag gtggtgccgc cgcagttcga ggcgcgcaac
    10141 gactttgata ttttccgcga gctgtgccgt cgctttaatc gcgaagaagc ctttaccgaa
    10201 gggctggacg aaatgggctg gctgaaacgc atctggcagg aaggtgtaca acaaggcaaa
    10261 ggacgcggcg ttcatctgcc agcgtttgat gacttctgga ataacaaaga gtacgtcgag
    10321 tttgaccatc cgcagatgtt tgttcgccac caggcattcc gcgaagatcc ggatctcgaa
    10381 ccgctgggca cgccgagtgg cctgattgag atctactcga aaaccatcgc cgatatgaac
    10441 tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa tcgaacgctc ccacggtggg
    10501 cccggctcgc agaagtatcc gttgcatctg caatctgtgc atccggattt ccgacttcac
    10561 tcgcagttat gtgagtcgga aactctgcgt cagcaatata cggtagcggg taaagagcca
    10621 gtgttcatta acccgcagga tgccagcgcg cgcggtattc gtaacggtga tgtggtacgc
    10681 gtctttaacg ctcgcggtca ggtgttggca ggggcagtag tttctgaccg ctatgcaccc
    10741 ggcgtggcac gaattcacga aggggcatgg tacgatccag ataaaggcgg cgagcctggt
    10801 gcgctgtgca aatacggtaa ccccaacgtg ctgaccatcg acatcggtac ttcgcagctg
    10861 gcgcaggcga ccagtgcgca cactacgctg gtggaaattg agaagtacaa tggagcagtg
    10921 gagcaggtga cggcgtttaa cggccctgtg gagatggtgg cgcagtgcga atatgttccc
    10981 gcgtcgcagg tgaaatcatg accacgctga cagcacaaca gattgcctgt gtttacgcct
    11041 ggctggcgca gttgttctcc cgtgaactgg acgatgaaca actgacgcaa atcgccagtg
    11101 cgcagatggc tgaatggttt tcgttgctga aaagcgaacc gccgctggct gcggcggtga
    11161 atgagctgga aaactgtatt gccacgctga cagtacgtga cgatgcccgt ctggaactgg
    11221 ccgctgactt ttgcggcctg tttctgatga ccgacaaaca agcggcgctg ccgtatgcat
    11281 cggcctacaa acaggacgag caagagatta aacgcttgtt agttgaggca gggatggaaa
    11341 ccagcggcaa tttcaacgaa ccggcagatc atctggcgat ctatctcgaa ttgctcagcc
    11401 atctgcattt ttcgctggga gaggggaccg ttcctgcgcg aagaatcgac agtttgcggc
    11461 aaaaaacact gacggcgctg tggcaatggt taccagagtt tgttgtgcgt tgtcgtcagt
    11521 atgacagctt tggtttttac gcggcactaa gccagttatt gctggtgtta gtggagagcg
    11581 accaccaaaa cagataacgt cgtttgtgcg cctgaaaaga cgcgtttagc gtcgcatcag
    11641 gcattatggg gcagttgccg gatgcgtcat cacatccggc aaacacacat cacggatgag
    11701 ctacaaaccg ggaaagtcgc tggcgcagca ggcggttttc ctgcttcagg tgcgcaatat
    11761 catccattaa cgtcagcgcc accgcgatcc caggccagtc cagagccagt tcatgacgca
    11821 ggcgtactgc gcgttgcacc acgatggcgg catggtcgtc aaatacccag gttgtttcct
    11881 ggatctcacg cggttcaacc acccccaaac cgacaatttc attcaactcc tcttcagaga
    11941 tgccggtatg caggcaaaat tcggtaatag taaaagtcac cgtaacatta gccattatgc
    12001 tttcccccaa tctttacgtg gatcaaaaga cgactgggcg tctgccagtt gctgccacag
    12061 cgcggcagtg ttttcatccg gtttcggcgg catcacgatt ttcagtaccg catacagatc
    12121 gccggtctgt tttttgctca ccagaccttt gcctttaacg cgcaatcgtt gcccggcctg
    12181 gctgcctggc gggatagtca gcaaaatgct ttctttcagt gttggaacgg tgactttagc
    12241 acccagcgcc gcttcccacg ggctaaccgg caccacaatt tccagatcct ggccgacaat
    12301 atcgaacagc ggatgtggcg caatatgaat caccagccac aaatcgccat ttggaccacc
    12361 gttttcgccc ggcgtcccct ggcctttcag acggatgcgt tgaccattgc cgacgcccgc
    12421 cgggatcttc acattcagcg ttttcgggat ttcctgttcg atcatgccaa aggcgttata
    12481 aaccggcagg ttatagctga tggtacgctt atgctcagtc agcgtttctt cgaggaatac
    12541 cgccacttcg atttcaatat cgtggccgcg tgcggcgggg cgttgacggc tctggcgggc
    12601 atgctgaccg aaaattgacg agaagatatc gtcaaaatct tcggcattaa aactctgacc
    12661 gtcgccatga tggaactgac ggttaaattg cggatcgttg cgatgttgcc acatctgatc
    12721 atactcagcg cgacgttgtt catcacttaa cacttcccag gcttcagcga cctctttgaa
    12781 gcgggcttcg gcatccggtt ctttgctgac atcaggatgg tatttacggg ccagtcgacg
    12841 ataggcggtc ttgattgtct tgagatcgtc cgtcggtttc acgcccatga tggcgtaata
    12901 attctttaat tccatagcgt tatctcgcgt aaatcaacac aaattgaagg aacccctgta
    12961 aggtaactcc tataagtgta gggtaatcct caaaatttca tatgccaaca cagaatatgt
    13021 tattgaaatc atcgcggaga ggaggtcgcc atcaagatgg gttgctgaac atattttaaa
    13081 caggtgaaaa agggtgagcg atttttgata gttgaaccag gcactttaag tttaactggg
    13141 gcgtcattat ttattaaatt ttatagacgc tatatatggg tagtaatata catggaatta
    13201 gttgcactgc aaataattat ttgaaacagg cctggaacga tataaaaaat gagtacgaaa
    13261 aaaatcaaac atattcaatc acgctttttg aaaacacact ggtgtgtttt atgcggttat
    13321 acaatgaact cagacgtaaa gtaaatgaag aggatactcc atgtctggaa tgtgaatcac
    13381 tagaaaaaga atttgaggaa atgcagaatg ataatgatct atcattattt atgagaacat
    13441 tgcgtactaa tgatacacaa atttattcag gggtttcagg aggtattaca tatactatac
    13501 aatatgttca aaatgttgat attgttagag tgtccttgcc gggcagaggt tcagagtcta
    13561 tcacagattt taaaggttat tattggtatg gctttatgga atatattgaa aacattactg
    13621 cgtgtgatga tgttttttct gagtattgtt ttgatgatga aaatatgagt atccagccag
    13681 aacagataaa tacg
//
LOCUS       DAZGVQ010000133        13637 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000133 DAZGVQ010000000
VERSION     DAZGVQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13637
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.134"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(165..1028)
                     /gene="potB"
                     /locus_tag="ACHLAG_003885"
     CDS             complement(165..1028)
                     /gene="potB"
                     /locus_tag="ACHLAG_003885"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFP0657023.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDD"
     gene            complement(1012..2148)
                     /gene="potA"
                     /locus_tag="ACHLAG_003886"
     CDS             complement(1012..2148)
                     /gene="potA"
                     /locus_tag="ACHLAG_003886"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFP0657024.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            2398..3627
                     /gene="pepT"
                     /locus_tag="ACHLAG_003887"
     CDS             2398..3627
                     /gene="pepT"
                     /locus_tag="ACHLAG_003887"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFP0657025.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            complement(3773..4894)
                     /gene="roxA"
                     /locus_tag="ACHLAG_003888"
                     /gene_synonym="ycfD"
     CDS             complement(3773..4894)
                     /gene="roxA"
                     /locus_tag="ACHLAG_003888"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFP0657026.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(4970..6430)
                     /gene="phoQ"
                     /locus_tag="ACHLAG_003889"
     CDS             complement(4970..6430)
                     /gene="phoQ"
                     /locus_tag="ACHLAG_003889"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFP0657027.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEKHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(6430..7101)
                     /gene="phoP"
                     /locus_tag="ACHLAG_003890"
     CDS             complement(6430..7101)
                     /gene="phoP"
                     /locus_tag="ACHLAG_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFP0657028.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            complement(7269..8639)
                     /gene="purB"
                     /locus_tag="ACHLAG_003891"
     CDS             complement(7269..8639)
                     /gene="purB"
                     /locus_tag="ACHLAG_003891"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFP0657029.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(8643..9284)
                     /gene="hflD"
                     /locus_tag="ACHLAG_003892"
     CDS             complement(8643..9284)
                     /gene="hflD"
                     /locus_tag="ACHLAG_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFP0657030.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(9320..10426)
                     /gene="mnmA"
                     /locus_tag="ACHLAG_003893"
                     /gene_synonym="trmU"
     CDS             complement(9320..10426)
                     /gene="mnmA"
                     /locus_tag="ACHLAG_003893"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFP0657031.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(10480..10941)
                     /gene="nudJ"
                     /locus_tag="ACHLAG_003894"
     CDS             complement(10480..10941)
                     /gene="nudJ"
                     /locus_tag="ACHLAG_003894"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFP0657032.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(10951..11604)
                     /gene="rluE"
                     /locus_tag="ACHLAG_003895"
     CDS             complement(10951..11604)
                     /gene="rluE"
                     /locus_tag="ACHLAG_003895"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFP0657033.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            11776..13026
                     /gene="icd"
                     /locus_tag="ACHLAG_003896"
     CDS             11776..13026
                     /gene="icd"
                     /locus_tag="ACHLAG_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFP0657034.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(13129..13452)
                     /locus_tag="ACHLAG_003897"
     CDS             complement(13129..13452)
                     /locus_tag="ACHLAG_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFP0657035.1"
                     /translation="MKWIVIDTIIQPSCGISFSAIWGNMKMIIWYQSTIFLPPGSIFT
                     PVKSGIILKDKEYPITIYNIAPFNKDLWSLLKSSQECPPGESKITNKCLHNSCIIKIC
                     PYGLK"
BASE COUNT         3438 a         3374 c         3423 g         3402 t
ORIGIN      
        1 ttttatacta tttgttgttt tttcagcgat cagataaaag ggcgctgaaa aaaacattct
       61 ccaaaattag attataacta tttgatttta tggatagagg catgcttttt acgcaaaagt
      121 aaaaagcatg ccaaatttgc tattaactct ttgaatattc agcactaatc atctatttcg
      181 gttgccttct tattcagcag acgagaagcg cgccagtaaa ccagcaacat caggcccatt
      241 acgatagtca gcgtaatgct ggtggctgca ccaaacggcc agtcacgaat attgaggaac
      301 tggaccttga tgacgttacc gatcagcagg tttttcgcac cgcccatcag gtcggataca
      361 tagaacaggc ccatcgctgg cagcatcacc agcagacatc cggcaataat tcccggcatc
      421 gtcagtggaa taatgatacg gataaaagtc tgtaacttgc tggcaccgag atcgcgcgcc
      481 gcctcaagca acggcttatc cagtttttcg atactggagt acagcggcat caccataaac
      541 ggtagcagaa tgtaaaccag accgataatc accgcactgg gcgtgaacat gatacgaatt
      601 ggtgtgtcga taacgcccag ccagagcaaa aactcgttga gatagccttt ggtgctgagg
      661 aaaattttca gcccgtagat gcgaatcagt gagttggtcc agaagggaac aatcagcaga
      721 aacagcagca gcggacgcac cttgtgtggt agcttcgcta gaaaccaggc aaacgggtag
      781 cccagcacca ggcaggcgag ggtggcgatc agcgccatat tcagcgagtg caatagcact
      841 tcaaaataga gcggatcgag cagacgcgtg tagttatcca gcgtaaagac cattttgacg
      901 aaactggcgt cgtcgcgggt caaaaagctg gtgccaatga tcatcaggtt gggcagaaag
      961 acaaacaaca caagccaacc gacaatagtg acaatcacta cattctggaa cttacttgtg
     1021 ttcttcatca gccagtacga cctcccagct ttctacccaa ttaatggcca ttttttggtc
     1081 gagagagtgg tcaaagtcag ggtcgtcttc attgaagaat tcgctgacca tcaccatctt
     1141 gccattttcc agttcaacaa ccgactccag cgtcatgcct ttgtagttac gctcacgaac
     1201 gtaaccaatc agcccttcag cgtggttgtc gtcgttaatc tcttcaacac gtaagtcttc
     1261 cgggcgcagc agaacatgca gtttttgccc cggttcaacg gcgaagttaa cgtagatatt
     1321 acattcgcgg ccttcaacgt tggcgcgtac gcgctgctcg tctagacgtt cgatgacagt
     1381 ggcgttaaac atattgattt cgccaatgaa gccggcaaca aacaggtttt tcggctcttc
     1441 gtaaatttcg cgcggtgtac catcttgctc aatacgaccc tcgcgcatca ccacaatcct
     1501 gtctgacatg gtgagcgctt cttcctgatc gtgagtgaca aagacgaagg taataccaag
     1561 cttacgctgt aacgctttca gctcgttctg catttgctta cgcagtttgt aatccagcgc
     1621 tgagagcgac tcatccagca acaacagacg aggcttgtta accaccgcgc gagcaatggc
     1681 gacgcgttgc tgttgaccac cagagagctg atgcggttta cgttgagcga aggtttccag
     1741 ttgcaccatc cgcagggctt ccatcacgcg gggcgtaatt tcagcagcgg gggttttttg
     1801 catgcgcaac ccaaaggcca cattttcgaa cacggtcatg tgggggaaaa gtgcgtagct
     1861 ttggaaaaca gtgttcacat agcggttttc cgccggaacg tgggtgatgt cctcgttatc
     1921 cagcatgatg cgtccggaat caacagtttc cagacctgca atcaggcgaa gaacggttgt
     1981 tttaccgcag ccagaagggc caagcagcgt gaggaactcg ccattgttga tagtcagatc
     2041 cagctgggga atgacctctt taccatcaaa gcatttgcga attcccgcca attgcaccag
     2101 cggtgaaagc gaactcggtt gtttattcaa ttttttactc tgtcccatgt aaacgcaacg
     2161 gatggcttac cgatgcgggg tttgtggtta accaccttgg tgactcttaa tgagggcggt
     2221 aattctacgg caaaccgctt gaatcgccaa tctttgttgt gaattactgg cttagcttta
     2281 tattcattaa ggtaatgctg ataaatattc ccgcttgcag gggtaaaagt gacctgacgc
     2341 aatatttgtc ttttcttgct tcttaataat gttgtcacaa aaagtgaggg tgactacatg
     2401 gataaactac ttgagcgatt tttgaactac gtgtctctgg atacccaatc aaaagcaggg
     2461 gtgagacagg ttcccagcac ggaaggccaa tggaagttat tgcatctgct gaaagagcag
     2521 ctcgaagaga tggggcttat caatgtgacc ttaagtgaga agggcacttt gatggcgacg
     2581 ttaccggcta acgtccctgg cgatatcccg gcgattggct ttatttctca tgtggatacc
     2641 tcaccggatt gcagcggcaa aaatgtgaat ccgcaaattg ttgaaaacta tcgcggtggc
     2701 gatattgcgc tgggtatcgg cgatgaagtt ttatcaccgg ttatgttccc ggtgctgcat
     2761 cagctactgg gtcagacgct gattactacc gatggtaaaa ccttgttagg tgccgatgac
     2821 aaagccggta ttgcagaaat catgaccgcg ctggcggtat tgcaacagaa aaacattccg
     2881 catggtgata ttcgcgtcgc ctttaccccg gatgaagaag tgggcaaagg ggcgaaacat
     2941 tttgatgttg atgccttcga tgcccgctgg gcttacactg ttgacggtgg tggcgtaggt
     3001 gaactggagt ttgaaaactt caacgccgca tcggtcaata tcaaaattgt cggtaacaat
     3061 gttcatccgg gcacggcgaa aggagtgatg gtaaatgcgc tgtcgctggc ggcacgtatt
     3121 catgcggaag ttccggcgga tgaaagcccg gaaatgacag aaggctatga aggtttctat
     3181 cacctggcga gcatgaaagg caccgttgac cgagccgata tgcactacat catccgtgat
     3241 ttcgaccgta aacagtttga agcgcgtaaa cgtaaaatga tggagatcgc caaaaaagtg
     3301 ggcaaagggt tacatcctga ttgctacatt gaactggtga ttgaagacag ttactacaat
     3361 atgcgcgaga aagtggttga gcatccgcat attatcgata tcgcccagca ggcgatgcgt
     3421 gactgcgata ttgaaccgga actgaaaccg atccgcggcg gtaccgacgg cgcgcagttg
     3481 tcgtttatgg gattaccgtg cccgaacctg ttcactggcg gttacaacta tcatggtaag
     3541 catgagtttg tgactctgga aggtatggaa aaagcggtgc aggtgattgt ccgtattgcc
     3601 gagttaacgg cgcaacggaa gtcgtgattg cccgatgcgt atgtggactg ccaattaccg
     3661 ggatgaaacc gttatattga tactggtcaa catacttatc gtgcctacag gcgttcagtg
     3721 catgtaggca cgataaggcg caatcagcgc cgcatccggc ttaaatccaa acttaccctt
     3781 cgaagaacca atacccgcta ttgaccagcg ccgcgagcat cgcgaggaat gacggatctt
     3841 ccagcgcatc gccaaaattc tccgcagtca gcgcaatgtt gctggcgagt gcatccagtg
     3901 ccggacggtg cggggaatcg atcttctcac cattggcata cacgtcgtcg ccaatgcgca
     3961 atacgcgcag accacccagg cgcaccagca catcaccttg tttcagcgca tcgtagattt
     4021 catctggctg ataaggcggt tccggcggcg cgatatccag ttcatgacgt gactgggata
     4081 taaactcgcc aaaccattgc ttaaagtgtt ccggctggtt gatcaattcg agcatcatct
     4141 cacgcagttt atccatctct tgcggcagaa catccgcagg atgagcgcga ggtggaacat
     4201 caggatcgct gtagtagttg ccgcccagtt cacgttgcag cacataatcg gcaaatccac
     4261 taatcagttc ccgcgtattt ggcgcgcgaa agcccacgga atagttcatc gcattttcca
     4321 gcgcgtagcc ttcatgcggg aatcctggcg gaatataaag aatatcacca ggctccagct
     4381 cttcatcgat gatggcttcg aacggatcga cctgtaacag atccggatgt gggcagtgct
     4441 gtttcatttg cagcttttcg cccactcgcc agcgacgacg tccggtaccc tgaatgataa
     4501 acacgtcgta ctgatcgaga tgcgggccga cgccgccgcc aggtacagaa aaagagatca
     4561 tcagatcatc aatacgccag tccggtagtt cacggaacgg tcgcatcagc gcggcggtcg
     4621 gctcatgcca gtggtttact gcctgtacca gtaatgacca gttggtttca ccgagatgat
     4681 cgtagctttc gaacgggccg tggctgacct gccatttgcc atcctggtga ctgaccagtc
     4741 gactgtcaac ttcgctttcc atcgccagac ccgccaactc gtctggagag atcgggtcaa
     4801 taaaattatt aaagccgcgt tttaacacca ccgggcgttt ctgccagtga cgttcaagaa
     4861 aatcgggcca gttaagagtg agttggtatt ccatgttaag catccacagg ctggtatctg
     4921 caaccgatta taacggatgc ttaacgtaat gcgtgaagta tggacatatt tattcatctt
     4981 tcggcgcaga atgctggcga ccaaaaatca cctccatccg cgcaccgccc agcatgctct
     5041 ctccggcgac gattttaccc tcatattgct cggtgatttc gcgggctacc gccagcccta
     5101 caccttgccc agggcgtaaa gtatcaaccc gttgaccacg gtcgaaaatg acctctcgct
     5161 tgcttaatgg aataccgggg ccatcatcct cgaccacaat atagagatgc tcgtcggttt
     5221 gccttgcaga aatttcgaca aactcgaggc aatatttaca ggcattatcc agcacgttgc
     5281 ccatcacctc gacaaaatcg ttctgctcac cgacaaagct gatctctggc gaaatatcga
     5341 gagagatatt gacccctttg cgttgataca ctttgttcag cgctgaggtg agattgtcca
     5401 gcagtggggc gaccggatgc agctcgcggc tgagcaatgt cccgccgcgc atactggcac
     5461 gatgcaggta gtagccaatt tgctgtgaaa tgcggctgat ttgctccagc attaccggct
     5521 cagcatcact gacgctcatc ttttcactac gcagagaacg cagcgtactt tgcagcaccg
     5581 ccagtggcgt tttcagacta tgggtcaggt cggtgagcgt cgtacggtat ttgtcgtaac
     5641 gttcgcgttc actttttaac aatcggttca ggtttcgtac cagactggtc agttctcgcg
     5701 ttgtggctgg attgagcaat tcgcggttat gtttttccag ttcgcggact tcttttgcca
     5761 gggcttcgat ggggcgtaaa ctccaccagg cggcgaccca cagcagcggg atcactaaca
     5821 gcagattggc tgagagcaca tagataaacc agctccagac catataggaa ctttttagct
     5881 ccaccggaat ggtatccacc accacaatgg ttaattttgg catccgcgat gttgccgggt
     5941 agacgtttac cgccaccgag tgggtcatct ccgcgtcgtc atcatcttcc cgcacttcct
     6001 gcaactgttg ctgtatcgaa tgatctccac tcagcaagag gctggtatcg ttaacatccg
     6061 cttcaatttc atgaaaacca ttcgatttca gccagtcagg ctggatcatc ttcatcagcc
     6121 agggcacgtc acgttgcgcc cataaaagct gcccgttctc atcataaatt agcgtcatgg
     6181 tggggctttg cttgtcgata ttttcgggta actcgacatg caacttattg ttttcccact
     6241 tcgcaagggt atagaacaga ttgctctcgc cacgtaacag ccgaaacgta gttttatcga
     6301 aactgacgct ataaccgatc agcgcgacca ttccgtaggc aagcgaaagc accagtacta
     6361 ccgctgccgt tgccaacaga aaacgtaccc gcagcgagag cgggaaaaaa agacgcagta
     6421 attttttcat cagcgcaatt cgaacaggta gccctggccg cgaacggtgg taatgacttc
     6481 ttgcggatat tgtgcctgaa tttttttgcg cagacgtccc atcagtacat caatggtatg
     6541 gctttcccgc agctccgcat ccggatagag ttggagcatt aacgaatctt tgctcaccac
     6601 tttgccatta ttgcgtatca acgtttccat aatggtgtat tcgaacgcgg tcagtttgat
     6661 cacttcgtca ttaatagata attcacggcg agagagatca acctgaaacg gtggtagcga
     6721 aatgacctgt gaagccagac cgctattacg ccgcattaat gcctgcattc gcgccatcac
     6781 ctcttcaata tgaaatggtt tagtcacgta atcatcagca ccagcactta atacttcgac
     6841 tttgtcctgc cagctttcac gggcggttaa taccagaatc ggcagtgaaa catcattgct
     6901 acgccagcgg cgaatcagtg acagaccgtc ctcgtctggc aatccgagat cgacaatcgc
     6961 aatatccggt aaatgttcat tgagataata atcggcttct ttggcatctt ctgcatcatc
     7021 gacctgatga ccagcatcct gaatctgaac tttaaggtgg tgacgtaaca acgcattgtc
     7081 ttcaacaacc agtacgcgca tttttatttc tccctgtctt aattattaaa atagtgtaac
     7141 gcgattatgt ggttatgggg gtaaacatta aataaaccag cggggagggg aggtaaagtg
     7201 aaaaaataaa aagcggataa tcttaataag caggccggac agcatcgcca tccggcactg
     7261 atacgagttt atttcagctc atcaaccatg gtgatggcgc gaccaatgta gtttgccggc
     7321 gtcatcgctt tcaggcgggc tttctcttct tctggcaacg ccagaccgtc gataaactgc
     7381 ttcatgcctt cggcgtcaac gcgcttacca cgagtcagct ctttcagctt ctcgtacggt
     7441 ttttcgatgc catagcgacg cataactgtc tgaattggtt ctgccagcac ttcccagttg
     7501 tgatccagct catccagcag atggtcacgg ttcacttcca gtttgctcac gcctttcagg
     7561 gtggattgat acgcaatcaa cgcataaccg atacccacgc cgaggttacg cagcacggtg
     7621 gagtcggtca ggtcacgctg ccagcgggaa accggcagtt tgcttgccag atgctgcaat
     7681 accgcgttgg aaaggcccag attcccttcg gagttttcga agtcgatcgg gttaacttta
     7741 tgcggcatgg tggaagaacc aatctcacca gcaatggttt tctgtttgaa gtggttaagg
     7801 gcgatataac cccagacgtc acggtcaaag tcgatcagaa tggtgttgaa gcgcgcaacg
     7861 caatcaaaca gttcggcaat gtagtcgtgc ggttcaatct gggtagtgta cgggttccat
     7921 tgaataccca gcgaggtgac gaactcttcg ctgaactgat gccagtcaac ttccgggtaa
     7981 gcggcgatgt gggcgttata gttaccgacc gcgccgttga ttttgccgag gatctccacc
     8041 tggttaagct ggcggtactg gcgctccata cggtaggcga cgtttgccat ctctttaccg
     8101 atggttgacg gcgtggctgg ctgaccgtgg gtacgagaca gcagcgggat atcgcggtac
     8161 tgaacggcga gatctttaat gccatcaatc agttgacgcc agtacggcag gatcacttca
     8221 tcacgcgcgg ttttcagcat taatgcgtgg gagaggttat tgatatcttc cgaagtacag
     8281 gcaaagtgga tgaattcaga aaccgcgtgc agttccggga tctccgccac tttttctttc
     8341 aggaaatact caaccgcttt aacgtcgtgg ttagtggtac gctcgatggt tttgatgcgt
     8401 gcggcatctt cttcgctgaa attggcgaca attgcatcaa ggtaaccgat tgcgtcggca
     8461 gcaaaagcag gaacttcctt gatcgctgcg tgcgcggcca gtttttgcag ccaacgtact
     8521 tcaacttgta cacggaattt cagcaaacca tattcgctga aaatcccgcg cagcgcgctg
     8581 actttatcgc cgtagcgtcc atcgacaggg gaaacggcgg tcagtgagga taattccata
     8641 gatcacaact ccggggttaa atgagcaaga atttgttttg cctgagtggt caggcgatta
     8701 cgagaaaaca tcagttgcag acgtccgccg ccgacctggt gccagagcac ggcggcgcga
     8761 atgcctgcca gcagggttgc gcgaactttc gcctgcactt gtgggctttg cagtacagca
     8821 ggggaaccgg tgacctgaat gcgcgggcca agcgggctga tcacatcaac atagatagca
     8881 gccatcgcgc tcatcagcgt ttcggactgt aaatcgaagt gttcgagctg gcgttgcagg
     8941 ccgttgatcc ggttgcccag agtgtcgagc gcgcctttcg ctgaggagag tttgcgctca
     9001 agcaccatca agctgagtgt gtagcgggtt aactcagcgt ttaagccctg gcgactgctg
     9061 gcattgagca cgccgagcag ggtttccagc ccgacgcgca ggttggcttc gctaccgcca
     9121 aaaaccgcca gcgtcgagct ggggttcata tcaataatac tgttgagtga gacgtgtagc
     9181 gcatcggcat cacaatgccc ctggtgagcg agttgttgca ccaggcgtgc cgactgacaa
     9241 ataccggcca gggcgagggt gatgtcatag taattctttg ccacgttcac tgcttccttg
     9301 ttttaagtaa agataataat cagaccggca gcggcagacg ctgctcaata ataccgccac
     9361 cgaggcacac ttcaccgtta tagaagacgg cagactggcc cggcgtcacg gcggcaaccg
     9421 gttcatcgaa aatcacttca atgcgatcat cgtccagcgc cttgacggtg caagggatat
     9481 cggtctggcg atagcgggtt tttaccgtgc aacgcatagt gccggtgaat ggttcgcgat
     9541 cgacccagtg caactgctgg gcaatcaacc cgacagacat cagccgcggg tgttcatggc
     9601 cctgagcgac aaccagaatg ttgttttcga cgtctttgtc caccacatac cacggttctt
     9661 cggtaccttc tttggtgcca ccgataccca gacctttacg ctgaccgaga gtgtgataca
     9721 tcagcccctg gtgctcacca atttcatcgc catcgacggt aatgattttg cccggttgcg
     9781 ccgggagata acggcccagg aactcgcgga atttacgttc gccaatgaag caaatgccgg
     9841 tagagtcttt tttcttcgcg gtgaccagac caagatcttc agcaatctta cgcacctgcg
     9901 gtttttccag ttcgccgacc gggaacaggc tttgcgcaat ctgctcatgg ctgagcgtat
     9961 aaaggaagta gctctggtct ttattgctgt ccagaccacg cagcaggcgg ctcttgccat
    10021 cgacatcggc ccgacgtacg taatgaccgg tagcgatata atcggcacct aaatcttcgg
    10081 cggcaaattc gaggaaggct ttaaatttga tctctttgtt gcacagaata tccggattcg
    10141 gcgtgcgacc ggctttatat tcggcaagga acagttcgaa gacgttgtcc cagtactcgg
    10201 cagcaaagtt aacggtgtgc agttcaatgc cgagcttgtc gcagacagcc tgggcatcag
    10261 ccagatccgc tgccgctgtg caatattcct caccgtcgtc ttcttcccag ttcttcataa
    10321 acaggccttc aacctgatat ccctgttgtt gcaacagcca ggcagaaacg gaggaatcga
    10381 caccgccgga catgccgacg attacttttt ttgcggtttc agacattgga tcactcacga
    10441 cattgaactt caaggcggcg tattctatca cgcagccttt tagatgacac cctttgtaaa
    10501 aggccagtta aaatcgccaa tcatctccag cggatagcgt tgcccgcttt gataacaacg
    10561 gatactttcc gccaccagcg gcgaacgaag atttgacgcc tgtaaaattt cttcggcgct
    10621 gacccaacga cagcagtcga tatcgctgtc atgaggctgg gtcgggcata tttgctcaag
    10681 ctcaatggca aagaggaaac gcaaaaacgg cgttttatct ggcgcaatcc actgatgcat
    10741 acgaataaag tgttgcggct gcgcgctgat gccggtttct tcccacagtt cacgggcggc
    10801 ggcttccact aaggtttcat cggcttccag atgcccggca ggttggttcc ataacgcttt
    10861 accattaatc gtctcttcaa cgactaaaaa tttgccttct gcgtgcacca cgcaagcaac
    10921 ggtaacgtgc ggtttaaaca ttttgcatcc ttaatctgtc acttctcgcc attcgccatt
    10981 ggcaagatta tccaaagagt aatctcccat cgcatagcga atcagtcgca gcgtggggaa
    11041 accaacatgg gcggtcatgc ggcgcacctg gcgattacgt ccttcatata aggtgatctt
    11101 cagccagctg gtgggaatac ttttgcgttc acgaattggt ggattccgcg gccataacca
    11161 cgcgggttcg tcaaccagct ccgcgcctgc gggcagggta gggccatcat ttaaggttac
    11221 gccattgcgc aaggcttcaa gtgcgtcttg tgtgggaata ccttcaacct gcacataata
    11281 gattttcccg gtgcgtttgc ccggctgggt taaacgcgcc tgcaacgcgc cgttattggt
    11341 cagcaccagt aacccttcgc tatcgcggtc aagacgacct gctgcataaa cgccctgaac
    11401 cgggatgaat tcttttaatg ttttgcgtcc ggcttcatcg gtgaactgcg gaagaacatc
    11461 gtagggttta ttgaacagga tcacacgcgt gggctggttt tcaggtttac gcctggtaga
    11521 acgttgcgag ctgaatcgct taacctggtg atttctaaaa gaagtttttt gcatggtatt
    11581 ttcagagatt atgaattgcc gcattatagc ctaataacgc gcatctttca tgacggcaaa
    11641 caatagggta gtattgacaa gccaattaca aatcattaac aaaaaattgc tctaaagcat
    11701 ccgtatcgca ggacgcaaac gcatatgcaa cgtggtggca gacgagcaaa ccagtagcgc
    11761 tcgaaggaga ggtgaatgga aagtaaagta gttgttccgg cacaaggcaa gaagatcacc
    11821 ctgcaaaacg gcaaactcaa cgttcctgaa aatccgatta tcccttacat tgaaggtgat
    11881 ggaatcggtg tagatgtaac cccagccatg ctgaaagtgg tcgacgctgc agtcgagaaa
    11941 gcctataaag gcgagcgtaa aatctcctgg atggaaattt acaccggtga aaaatccaca
    12001 caggtttatg gtcaggatgt ctggctgcct gctgaaaccc ttgatctgat tcgtgaatat
    12061 cgcgttgcca ttaaaggtcc gctgaccact cctgttggtg gcggtattcg ctctctgaac
    12121 gttgccctgc gccaggaact ggatctctac atctgcctgc gtccggtacg ttactatcag
    12181 ggcactccaa gcccggttaa acaccctgaa ctgaccgata tggttatctt ccgtgaaaac
    12241 tcggaagaca tttatgcggg tatcgaatgg aaagcagact ctgccgacgc cgagaaagtg
    12301 attaaattcc tgcgtgaaga gatgggggtg aagaaaattc gcttcccgga acattgtggt
    12361 atcggtatta agccgtgttc ggaagaaggc accaaacgtc tggttcgtgc agcgatcgaa
    12421 tacgcaattg ctaacgatcg tgactctgtg actctggtgc acaaaggcaa catcatgaag
    12481 ttcaccgaag gcgcgtttaa agactggggc taccagctgg cgcgtgaaga gtttggcggt
    12541 gaactgatcg acggcggccc gtggctgaaa gttaaaaacc cgaacaccgg caaagagatc
    12601 gtcattaaag acgtgattgc tgatgcattc ctgcaacaga tcctgctgcg tccggctgaa
    12661 tatgatgtta tcgcctgtat gaacctgaac ggtgactaca tttctgacgc tctggcagcg
    12721 caggttggcg gtatcggtat cgcccctgga gcaaacatcg gtgacgaatg cgccctgttt
    12781 gaagccaccc acggtactgc gccgaaatac gccggtcagg acaaagtaaa ccctggctct
    12841 attattctct ccgctgagat gatgttacgc catatgggct ggactgaagc cgcagacctg
    12901 attgttaaag gtatggaagg cgcaatcaat gcgaagaccg taacctatga cttcgagcgt
    12961 ctgatggaag gcgctaaact gctgaaatgt tcagaatttg gtgacgcgat catcaagaac
    13021 atgtaatcac tacatgtgtt taatattgca acgggcgtat aacacgcccg ttgttttatt
    13081 tatgtaggta ttattaatag catatcgagc atatttatat aaaacccttt acttgagccc
    13141 atatgggcat atttttataa tgcaactatt atgcaaacat ttatttgtta ttttgctttc
    13201 tcctggagga cactcttgac tgcttttgag tagactccat aaatccttgt tgaatggtgc
    13261 gatgttataa atagtaatag gatattcttt atctttaagg ataattccag atttaaccgg
    13321 tgtaaatata ctgccaggag ggagaaatat agtagattga taccagatga tcattttcat
    13381 attaccccat atggctgaaa aagatatgcc gcatgaaggt tgaattatcg tgtcaattac
    13441 tatccacttc atttgttatg tcttatccca cggtatttaa tatagttcat ttggatgttc
    13501 atttctttat tttgcatatg agtatattac cccttcaaaa aataaattaa ttaaaacgat
    13561 tgcttatata aaacaaaatt taaagcaagg aatctcaatg gatgttaaac aaaatgagat
    13621 ttagtgaaaa caataaa
//
LOCUS       DAZGVQ010000134        13340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000134 DAZGVQ010000000
VERSION     DAZGVQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.135"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            182..2605
                     /gene="pgaA"
                     /locus_tag="ACHLAG_003898"
     CDS             182..2605
                     /gene="pgaA"
                     /locus_tag="ACHLAG_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFP0657036.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2614..4632
                     /gene="pgaB"
                     /locus_tag="ACHLAG_003899"
     CDS             2614..4632
                     /gene="pgaB"
                     /locus_tag="ACHLAG_003899"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFP0657037.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            4625..5950
                     /gene="pgaC"
                     /locus_tag="ACHLAG_003900"
     CDS             4625..5950
                     /gene="pgaC"
                     /locus_tag="ACHLAG_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFP0657038.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLTTTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            5952..6365
                     /gene="pgaD"
                     /locus_tag="ACHLAG_003901"
     CDS             5952..6365
                     /gene="pgaD"
                     /locus_tag="ACHLAG_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFP0657039.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6415..7203)
                     /gene="phoH"
                     /locus_tag="ACHLAG_003902"
     CDS             complement(6415..7203)
                     /gene="phoH"
                     /locus_tag="ACHLAG_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFP0657040.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(7822..9093)
                     /gene="efeB"
                     /locus_tag="ACHLAG_003903"
     CDS             complement(7822..9093)
                     /gene="efeB"
                     /locus_tag="ACHLAG_003903"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFP0657041.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKDKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9099..10226)
                     /gene="efeO"
                     /locus_tag="ACHLAG_003904"
     CDS             complement(9099..10226)
                     /gene="efeO"
                     /locus_tag="ACHLAG_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFP0657042.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10284..11114)
                     /gene="efeU"
                     /locus_tag="ACHLAG_003905"
     CDS             complement(10284..11114)
                     /gene="efeU"
                     /locus_tag="ACHLAG_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFP0657043.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVPGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGVFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            11150..11452
                     /locus_tag="ACHLAG_003906"
     CDS             11150..11452
                     /locus_tag="ACHLAG_003906"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657044.1"
                     /translation="MQRKVNRSDSDYHYGKEKYKRNVYKMMLECKQREVMPRDINDYS
                     RVERWDCLQRTMCSCRMPRKRRIQPTKLSRLNASELPCKTDKRVYQAISSIRDYQI"
     gene            complement(11657..13165)
                     /gene="putP"
                     /locus_tag="ACHLAG_003907"
     CDS             complement(11657..13165)
                     /gene="putP"
                     /locus_tag="ACHLAG_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFP0657045.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
BASE COUNT         3525 a         3406 c         3139 g         3270 t
ORIGIN      
        1 ttcgggcgag aaaaggattt tatatggaca ctctgctcat catttcttct tctcatcatc
       61 aacaattcac gtctctcttc cgcgtttaat aacggattat gaggtgcaaa aatatctttc
      121 ttttcagtta cctgtaatta gatacagaga gagattttgg caatacatgg agtaatacag
      181 gatgtattca agtagcagaa aaaggtgccc gaaaaccaaa tgggctttga aacttcttac
      241 tgccgcattt ttagcagcga gtcccgcggc gaagagtgct gttaataacg cctatgatgc
      301 attgattatt gaagctcgca agggtaatac tcagccagct ttgtcatggt ttgcactaaa
      361 atcagcactc agcaataacc aaattgctga ctggttacag attgccttat gggccgggca
      421 agataaacag gttattaccg tttacaaccg ctaccgtcat cagcaattac cagcgcgtgg
      481 ttatgcagct gtcgccgtcg cttatcgtaa cctgcaacaa tggcaaaact cgcttacact
      541 gtggcaaaag gcgctctctc tggagccgca aaataaggat tatcaacggg gacaaatttt
      601 aaccctggca gatgctggtc actatgatac tgcgctggtt aaacttaagc agcttaactc
      661 tggagcaccg gacaaagcca atttactcgc agaagcctat atctataaac tggcggggcg
      721 tcatcaggat gaattacggg cgatgacaga gtcattacct gaaaatgcat ctacgcaaca
      781 atatcccaca gaatacgtgc aggcattacg taataatcaa cttgctgccg cgattgacga
      841 tgccaattta acgccagata ttcgcgctga tattcatgcc gaactggtca gactgtcgtt
      901 tatgcctacg cgcagtgaaa gtgaacgtta tgccattgcc gatcgcgccc tcgcccaata
      961 cgctgcatta gaaattctgt ggcacgataa cccagaccgt actgcccagt accagcgtat
     1021 tcaggttgat catcttggcg cgttattaac tcgcgatcgt tataaagacg ttatttctca
     1081 ctatcagcga ttaaaaaaga cggggcaaat tattccgccc tgggggcaat attgggttgc
     1141 atcggcttat ctcaaagatc atcagccgaa aaaagcacag tcaataatga ccgagctctt
     1201 ttatcacaag gagaccattg ccccggattt atccgatgaa gaacttgcgg atctctttta
     1261 cagccacctg gagagtgaaa attatccggg cgcgctaact gtcacccaac ataccattaa
     1321 tacttcgccg cctttccttc ggttaatggg cacgcctacg agcatcccga atgatacctg
     1381 gttacagggg cattcgtttc tctcaaccgt agcaaaatat agtaatgatc ttcctcaggc
     1441 tgaaatgaca gccagagagc ttgcttataa cgcaccagga aatcagggac tgcgcattga
     1501 ttacgcgagt gtgttacaag cccgcggttg gcctcgtgca gcagaaaatg aattaaaaaa
     1561 agcagaagtg atcgagccac gtaatattaa tctggaggtt gaacaagcct ggacagcatt
     1621 aacgttacaa gaatggcagc aggcagctgt cttaacgcac gatgttgtcg aacgtgaacc
     1681 gcaagatccc ggcgttgtac gattaaaacg tgcggttgat gtacataatc ttgcagagct
     1741 tcgtatcgct ggctcaacag gaattgatgc cgaaggcccg gatagtggta aacatgatgt
     1801 cgacttaacc accatcgttt attcaccacc gctgaaggat aactggcgcg gttttgctgg
     1861 attcggttat gccgatggac aatttagcga aggaaaaggg attgttcgcg actggcttgc
     1921 gggtgttgag tggcggtcac gtaatatctg gctcgaggca gagtacgctg aacgcgtttt
     1981 caatcatgag cataaacccg gcgcgcgcct gtctggctgg tatgatttta atgataactg
     2041 gcgtattggt tcgcaactgg aacgcctctc tcaccgcgtt ccattacggg caatgaaaaa
     2101 tggtgttaca ggcaacagtg ctcaggctta tgttcgctgg tatcaaaatg agcggcgtaa
     2161 gtacggtgtc tcctgggctt tcactgattt ttccgacagt aaccagcgtc atgaagtctc
     2221 acttgagggt caggaacgca tctggtcttc accatatttg attgtcgatt tcctacccag
     2281 tctgtattac gaacaaaata cagaacacga taccccatac tacaacccta taaaaacgtt
     2341 cgatattgtt ccggcatttg aggcaagcca tttgttatgg cgaagctatg aaaatagctg
     2401 ggagcaaata ttcagcgcag gtgttggtgc ctcctggcaa aaacattatg gcacggatgt
     2461 cgtcacccaa ctcggctacg ggcaacgcat tagctggaat gacgtgattg atgctggcgc
     2521 aacgctacgc tgggaaaaac gaccttatga cggtgacaga gaacacaact tatacgttga
     2581 attcgatatg acattcagat tttaaggata aatatgttac gtaatggaaa taaatatctc
     2641 ctgatgctgg tgagtataat tatgctcacc gcgtgcatta gccagtcaag aacatcattt
     2701 ataccgccac aggatcgcga atctttactc gccgagcaac cgtggccgca taatggtttt
     2761 gtagcgattt catggcataa cgttgaagac gaagctgccg accagcgttt tatgtcagtg
     2821 cggacatcag cactgcgtga acaatttgcc tggctgcgcg agaacggtta tcaaccggtc
     2881 agtattgctc aaattcgtga agcacatcga ggaggaaaac cgctaccgga aaaagctgta
     2941 gtgctgactt ttgatgacgg ctaccagagt ttttataccc gcgtcttccc aattcttcag
     3001 gccttccagt ggcctgctgt atgggccccc gtcggcagtt gggtcgatac gccagcggat
     3061 aaacaagtaa aatttggcga tgagttggtc gatcgagaat attttgccac gtggcaacaa
     3121 gtgcgagaag ttgcgcgttc ccggctcgtt gagctcgctt ctcatacatg gaattctcac
     3181 tacggtattc aggctaatgc caccggcagc ttattgcctg tatatgtaaa tcgtgcatat
     3241 tttactgacc acgcacggta tgaaaccgca gcagaatacc gggaaagaat tcgtctggat
     3301 gctgtaaaaa tgacggaata cctgcgtaca aaggttgagg taaatccaca cgtttttgtt
     3361 tggccttatg gcgaagcgaa tggcatagcg atagaggaat taaaaaaact cggttatgac
     3421 atgttcttca cccttgaatc aggtttggca aatgcgtcgc aattggattc cattccgcgg
     3481 gtattaatcg ccaataatcc ctcattaaaa gagtttgccc agcaaattat taccgtacag
     3541 gaaaaatcac cacaacggat aatgcatatc gatcttgatt acgtttatga cgaaaacctc
     3601 cagcaaatgg atcgcaatat tgatgtgcta attcagcggg tgaaagatat gcaaatatca
     3661 accgtgtatt tgcaggcatt tgctgatccc gatggtgatg ggctggtcaa agaggtctgg
     3721 tttccaaatc gtttgctacc aatgaaagca gatattttta gtcgggttgc ctggcaatta
     3781 cgtacccgct caggtgtaaa catctatgcg tggatgccgg tattaagctg ggatttagat
     3841 cccacattaa cgcgagtaaa atacttacca acaggggaga aaaaagcaca aattcatcct
     3901 gaacaatatc accgtctctc tcctttcgat gacagagtca gagcacaagt tggcatgtta
     3961 tatgaagatc ttgccggaca tgctgctttt gatggcatat tgttccacga tgatgctttg
     4021 ctttcagatt atgaagatgc cagtgcaccg gctatcacgg cttatcagca agcaggcttt
     4081 agcgggagtc tgagcgaaat tcgacaaaac ccggagcaat ttaaacagtg ggcccgcttt
     4141 aaaagtcgtg cgttaactga cttcacttta gaacttagtg cgcgcgtaaa agccattcgc
     4201 ggtccacata ttaaaactgc acgaaatatt tttgcacttc cggtaataca acctgaaagt
     4261 gaagcctggt ttgcacagaa ttatgctgat ttcctaaaaa gctatgactg gaccgctatt
     4321 atggctatgc cttatctgga aggtgtcgca gaaaaatcgg ctgaccaatg gttaatacaa
     4381 ttgaccaatc aaattaaaaa catccctcag gctaaagaca aatctatttt agaattacag
     4441 gcacaaaact ggcagaaaaa tggtcagcat caggctattt cttcgcaaca actcgctcac
     4501 tggatgagcc tattacaact gaatggagtg aaaaactatg gttattatcc cgacaatttt
     4561 ctgcataacc aacctgaaat agaccttatt cgtcctgagt tttcaacagc ctggtatccg
     4621 aaaaatgatt aatcgcatcg tatcgttttt tatattatgt ctggtgttat gcatacccct
     4681 atgcgtagcg tactttcact ctggtgaact gatgatgagg ttcgttttct tctggccgtt
     4741 ttttatgtcc attatgtgga ttgttggcgg cgtctatttc tgggtctatc gtgaacgcca
     4801 ctggccgtgg ggagaaaacg caccagctcc ccagttgaaa gataatccgt ctatctccat
     4861 tatcattccc tgttttaatg aggagaaaaa cgttgaggaa accatacacg ccgctttagc
     4921 acagcgttat gagaacattg aagttattgc cgtaaatgac ggttcaacag ataaaacccg
     4981 tgccatcctg gatcgcatgg ctgcacaaat tccccatttg cgggtcattc atctggcgca
     5041 aaaccagggg aaagccattg cgcttaaaac cggagctgcc gcggcgaaaa gtgaatatct
     5101 ggtgtgcatt gatggcgatg cgttattaga ccgcgatgcg gcggcatata ttgtggaacc
     5161 gatgttgtac aacccgcgtg tgggtgccgt aaccggtaat cctcgtattc gaacacgttc
     5221 taccctggtg ggtaaaattc aggttggcga gtattcctca attattggtt tgatcaagcg
     5281 aacccagcgt atctatggaa acgtatttac cgtttccggt gttattgccg catttcgtcg
     5341 cagcgccctg gcagaagtgg gttactggag tgacgatatg atcaccgaag atattgatat
     5401 tagctggaag ctgcagttga atcagtggac gattttttac gagccacggg cgctgtgctg
     5461 gatattaatg cctgaaacgt taaaagggct gtggaaacag cgcctgcgct gggctcaggg
     5521 cggtgcagaa gtattcctca aaaatatgac aaggttgtgg cgcaaagaaa actttcgaat
     5581 gtggccgctg ttttttgaat actgcctgac gacaatatgg gccttcacct gcctggtcgg
     5641 tttcattatt tacgcagtcc aacttgccgg tgtaccgtta aatattgaat tgacacatat
     5701 cgctgcgaca catactgccg gaatattatt gtgtacgtta tgtttactgc aatttattgt
     5761 cagcctgatg atcgagaatc gctatgagca taatctgact tcatcgcttt tctggattat
     5821 ttggttcccg gttattttct ggatgctgag cctgacaacg acattggtat catttacacg
     5881 agtcatgttg atgcctaaaa agcaacgcgc ccgttgggta agtcccgatc gcgggattct
     5941 gagaggttaa tatgaacaat ttaattatta cgacccgaca atcaccagta cgtttactgg
     6001 ttgattatgt tgccacaacc atcttgtgga cattatttgc gttgttcata ttcttattcg
     6061 ccatggatct gctgacgggt tattactggc aaagcgaggc cagaagccga cttcagttct
     6121 attttttgct ggcagtggcg aatgccgtcg tgttaattgt ctgggcgctg tacaataagc
     6181 tgcgttttca aaaacagcag catcatgcag cctaccaata tacgccgcaa gaatatgcag
     6241 agagcttagc aatacctgat gagctctatc agcaactaca aaaaagccac aggatgagcg
     6301 tacacttcac cagccagggg caaataaaaa tggttgtttc agaaaaagcg ctagtccggg
     6361 cataaacacc caaaacaaag cccggttcgc ccgggctctg caccgataat acacttaact
     6421 gtatgcatgc agcgtacgtt ggcaaagtgc cgaacgtacg cagtcctctt taccgaaccg
     6481 gacgatccca accatttcat cttcttcgaa acgttccagc gcgtcactta atccggagca
     6541 cacgccgcga ggcaaatcgc attgcgtgat atcaccgttg acgataaccg tcacgttctc
     6601 cccgaggcgg gttaaaaaca ttttcatttg cgcggcagtc acattctgcg cctcgtcaag
     6661 aatgacgact gcattttcaa aggtacgtcc acgcatatag gcgaacggcg caatttccac
     6721 cttccctatt tccggtcgca ggcagtactg cataaaggaa gcccctaagc gccggaccag
     6781 cacgtcgtag accgggcgaa aatagggagc aaacttttct gcgatatctc caggtaagaa
     6841 gccaagatct tcatcggctt gcagaactgg acgggtgacg ataatcctgt cgacatcctt
     6901 atgtatcagg gcctctgccg cttttgctgc gctgatccag gtttttccgc acccggcttc
     6961 gcccgtggcg aatatcagct gcttactctc aatagccttc agatagtgca attgcgcttc
     7021 atttcgcgcg aggatgggcg aagtatcgcg actgtcgcgg gccataccaa tggcttctac
     7081 gccgcccatc tgcacaagcg aggtgaccga ttcttcttca cgctgcttat ggctgcgcga
     7141 atcccgtctc agcacacgtt ttgcctcacg acgagctttg atcactgctt tttgtcttcc
     7201 catggagagc accttgagtt gtttgtattc atcacacgcg ccgttggcag cgcgattatg
     7261 cgcacgaaca tcagagggtt ggcttccttg taagccatag tttgcttttg gataaaatgc
     7321 cgaaaaacgg ctacgcacac cgtttgcggc gtcggtaaca cgagtaaaga gaggaagtga
     7381 gaaaaataca gagtgacgaa gttgctaccg gaggagaaat ctccgcaagt ggtggagcgt
     7441 agcttaacaa gacattgtcc agaacaggac gttaccatcc gcgatctcca tagtgactga
     7501 ctatcactgc cgggaacttc cgctgctact taataattac aacagatctc gcattcattg
     7561 caacaatata ttcacttata tttaaccata aaacaccatt tcagtgacat tagtttctac
     7621 tggaaagatg acagagtgat gacagatatg aaaaaagctg tgtgctttca gcaggattcg
     7681 catcaggcat ctgcgcacga acgccggatg cggcgtgaac gccttatccg cctaccgaac
     7741 ccgcaccgat tgtaggcata agaagacgcg caagcatcgc atcaggcatc tgcgcaagaa
     7801 cgccttatcc gtctataaac attaaacccg caataacgcg cttccgagat aatcgttcgc
     7861 gtcctttact cccggcagcg caaaaaaata accgccgccg ataggtttaa cgtattcctc
     7921 cagcgcttcg ccattgagcc ttttttgtac agtcaggaag cctttttcca gatcgtgttg
     7981 gtaacagaca aacagtagcc ccatatccag ttgcccggag ttggtgacgc ccagtgaata
     8041 actgtagcca cgacgcagca tcaggctgga ctcactctcc gccgtgcggg gattcgccag
     8101 ccggatatgg ctgtccagcg caatcacctt cccttccgga tcgctggcgt aatcgggaac
     8161 atcatgctca tgctgcattc ccagcggcgc accggtttgc ttatcacgac caaaaatcgt
     8221 ctgctgttct ttcagcggag ttctgtccca aaattccact cgaaactgaa tcaagcgtac
     8281 tgcctgatag ctgccaccga ttgtccacgc tggctcctgc tgatctgccg ttacccacac
     8341 cactttttgc atcaacttat cattctggct atcgggattg gcagtgccgt ctttgaaacc
     8401 cagcaaatta atcggcgtct ctttgtcttt actacgcgcc gcgtgatcgg aaataaaccc
     8461 ttcccgcttc cagcgcacgc tgagcaaatc tggcgtatgt ttgatgatat cgcgcagcgc
     8521 atggatcacc gtgtcctggg tgttggcgca aatttgtagc aacacatcac catgacataa
     8581 cgccgcatcc agcgagtcgt tgggaaaacg cgtcatcttc tgcaactttt tcggcatctg
     8641 tggcgcaagg ccaaagcgct catcaaacaa tgagtgaccc accgataacg tgatggtgag
     8701 attatcgggc gcaatgtagc cgccaagaat gccggaatcg agtggtggca ggcgcggatt
     8761 tggcgtttct ggtgccgctc cgccctgagt cagaaaagca aaacgctgag tcaacaagcg
     8821 aaacaaccgt tcaagatcgg gtttatcgct ggcaagcaca tcaaacgcca ccagcatcat
     8881 tgcggcctgt tgtggcgtca ggatccctgc ctgatgctca ccataaaacg gctgtttctc
     8941 attgcgtgca tccggtgaaa gcgtacccgg cgcactttgc gttttttgtg catgagcgac
     9001 cggacaactt cccgccagcg ccagcgcacc aatgcctttc agtaaacgtc ggcgtgacgg
     9061 ttcattcacg ccgtttttat cttcatactg cataatgctt aatccaatcc cagcacaccg
     9121 cgaagttgcg ccagatcttc cgccagcgca gtaatcggtc ctttcagtgc attccggtcg
     9181 gcatcggtca atttgtcgta ggtttcaaaa ccgtctttag tacggtattt cgccagaatg
     9241 gtatcgacct ttttaaagtt ggcatcgact tttgccaaca gttccgggtt ggctttttgc
     9301 agttgtggac gcagcagatc gacaattttc tgcgagcctt caacgttagc ctggaaatcc
     9361 cacagatcgg tgtggctgta gcgatcttct tcaccgctga ttttgctggc tgccacttcc
     9421 tcaatcagtc cggctgcacc gccgaccact tttgaaggtg ggaaagccag ttcactgatg
     9481 cgtttttgca aatcgaccac atcggtataa agctggtcag cgtactgatc catgcctttg
     9541 gtggtgttgt cgccaaacaa tgctttttcc agacggtgga aaccggtgaa tttcggatcg
     9601 gcggcttttt gctcgtaatc atcttcacgg gcgtcaatgc tgccatccag atcggagaac
     9661 agttcagcaa tcggttcgat gcgctcatag tgctggcgcg tcggcgcata cagtgctttc
     9721 gctttttcga tatcgcctgc tttaatagcg tcagtaaagg ctttcgtgtc ggtcaccagc
     9781 tgcgtggttt ccgccatgac atacgcttta tatgcagtaa ttgcaccacc aagacttaac
     9841 agcgcatcac tttgcgccgc atccgccgtt gcctcacctt tgacgatcaa cttccctttc
     9901 gggttagtca gcagaccgca ggtcatatcg tattcgccag gctgtaaatt cgccgtcatt
     9961 ttctggctaa agccaggggc gatattttcc cgctcttcca ccaccatcac gcctttgagg
    10021 atctcccact ccagcgcctt ctggctgtgg ttctgaataa tgaactgtgt tttcccggcg
    10081 ttaaccgtaa tggtcatcgg ttcgcactgc ttatcggtca cggtcacctt gacctgcggc
    10141 acattagcgg cgttagccat aaaagcagaa gagaacagcg cagccacgct caactgcaat
    10201 gcgttacggc ggaagttaat ggtcatgacg agactatccc tttaaagtat gttgtaacta
    10261 agcaagagtt tgcgtcgtat ttgctacgcg gagcgagacg ctgtcgcccc tgcgcgtggt
    10321 ggcagggcaa atgccaccag cgccgggatg agataaataa accagacggc gacttcgctg
    10381 acgctcggtg cttcctgata gccaaaaacc ccttccatca gcgtgccaaa cagcgagtga
    10441 gttgagagga ccgcgctcat atcgaaggcg atttcctgaa agtggttcca caatccggct
    10501 tcatgaaatg cgcgaatggc accagccgcc agccctgcgg cgacgaagag aataaacagg
    10561 ctggtccatt taaaaaatgc accaagattg aggcgaatac cgccccagta gagcaggaag
    10621 ccgggcacca cggcagtagc aagaccgagc attgcaccca gcggcggcca gatcccgaca
    10681 tcttgttgaa atgccgccag caggaaaaag accgactcca gcccttccct tgcaacggca
    10741 aaaaagacca tcatcaccag cgcccagcca tgatgatttc cacgctgcaa tgcgctatcg
    10801 actgcctgtt ccagttgcac tttgacgttg cgcgacactt tgcgcatcca gaaaaccatc
    10861 caggtaagga tcaccacggc gatcaccgcc acgatacctt caaacagttc ctgttctttt
    10921 tgcggaaatt cgccggtggt ttcgttaatg aagataccca ggcccaggca caacgcagcg
    10981 gcaagcaaca cgccaatcca catcacacca atccatcggc ctcgctgggt acgcttaaga
    11041 tagctggcaa tcaaactgac aatcagcgcg gcttcaagtc cttcgcgcaa cataatgaga
    11101 aacggaacaa acatgccacc cacccttaaa cgtttttctg agtcaatcta tgcaaagaaa
    11161 agtaaatcgc agtgatagcg attatcatta cggtaaagaa aaatacaagc ggaatgttta
    11221 taaaatgatg ttggaatgta aacaaagaga ggtaatgcca agggatataa acgattattc
    11281 cagggtagaa agatgggatt gtttgcaaag aaccatgtgt tcttgccgga tgccgcgtaa
    11341 acgccgtatc cagccgacaa aactgtccag actcaatgca tcagagcttc catgtaaaac
    11401 tgataagcgg gtgtatcaag caatttcgtc catccgcgat tatcaaatct aactttttgc
    11461 ccggaacata ataacagctc gggtgttggt ggcgtcaaaa tcgctgaagc gttccccgaa
    11521 ggccgggaca gcagggggcg gcggcgattg gccgccccct gcgcgctccc tgcaccattc
    11581 aatttatatg gcgaggcaca ttaagggagc gcaacccgct gctgaagcca aacaaaaccg
    11641 ccgcaggcta agtcccttag ctttcctgca accgtgacgg cggagccgaa tgatagtgcg
    11701 catcggcctc ggcaaagcgt ttttgcatcg ccgctgacgg cgctttaccc agcaaactaa
    11761 acactacaat cccaatactg ccgaagataa agcccggaat aatttcgtag agtcccagcc
    11821 agccgaactg cttccagacg ataaccgtca gcgcaccgat gatcatcccc gccagcgcac
    11881 cgttgcgcgt catgcgtgac cacatcaccg agaacagcac caccggacca aacgccgcgc
    11941 caaagcctgc ccacgcgtag ctcactaagc ccagcacgcg gttttccggg tttgccgcca
    12001 gcgcaatcgc caccagcgcc accaccagca ccatcacacg ccctacccac accagctctt
    12061 tctggctggc atgtttacgc agaaacgctt tgtataaatc ttcggtaatc gcactggagc
    12121 acaccagcag ctggcagctt aacgttgaca ttaccgccgc cagaattgcc gacagcagaa
    12181 tcccggcaat ccacgggtta aacagaattt gcgccagttc gataaacaca cgttcagcgt
    12241 tctggtttac cgcacctgct accgccgggt gctcattaaa gtaagcaatc ccaaagaagc
    12301 cgacagccac cgcccctgcc aggcagagga tcatccaggt catactgata cgacgcgcat
    12361 ggacaatgct gtggtgagaa tccgccgcca taaaacgcgc cagaatatga ggctgaccga
    12421 agtaacccag cccccaaccc atcaatgaga taatggcaac aaagttcagc cctttgagca
    12481 tatccacgtt ttcgatgctc ttttgtttga tcacttccag cgagtcacca aagccaccga
    12541 cactgataat gacgataacc ggcgtcagga tcagggcaaa aatcatcagg ctggcctgta
    12601 cagtgtcagt ccagctcacc gcgaggaaac cgccaataaa ggtgtaaagg atcgtcgccg
    12661 cagcgcccgc ccacagagcc gtttcgtagc tcatgccaaa ggtactttca aacagacgcg
    12721 cgcctgccac aatgcccgaa gcgcaataaa tggtgaagaa cagcaaaata accagcgcag
    12781 agataatgcg caaaatgcgg cttttatctt caaagcgccc ggtgaaataa tccggcaatg
    12841 ttaaggcgtt attgttgtat tcggtatgca cacgcaaccg cccggccacc agcttccagt
    12901 tgatccacgc gcctaatgtc aggccaatgg cgatccagct ttcggaaatc ccggaaagaa
    12961 aaaccgcgcc cggcaacccc attaacagcc agccgctcat atcagacgca cccgccgata
    13021 atgccgtcac gaatggccca aggctacgac cgcccagaat atagtcgtcg aagtttttcg
    13081 ttgatcgcca ggcgataaac ccaatcaata tcatgccaaa gatatagaca caaaatgtca
    13141 ccaacatcgg tgtgctaata gccatctaaa gtctccaaaa aattattatc ggcaatgtcg
    13201 aaacttgccg ttatatctgc caccggaacg gggtaacaga gtttatgttt taccagggcg
    13261 accgtatcct gccggaagcg ctggttattc acaatcgatt taacacacca tttacattaa
    13321 attttagtgc tcagcgacac
//
LOCUS       DAZGVQ010000135        13263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000135 DAZGVQ010000000
VERSION     DAZGVQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.136"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            358..4818
                     /gene="gltB"
                     /locus_tag="ACHLAG_003908"
     CDS             358..4818
                     /gene="gltB"
                     /locus_tag="ACHLAG_003908"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFP0657046.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPHIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            4831..6249
                     /gene="gltD"
                     /locus_tag="ACHLAG_003909"
     CDS             4831..6249
                     /gene="gltD"
                     /locus_tag="ACHLAG_003909"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFP0657047.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            6433..7560
                     /gene="yhcG"
                     /locus_tag="ACHLAG_003910"
     CDS             6433..7560
                     /gene="yhcG"
                     /locus_tag="ACHLAG_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFP0657048.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(7620..8084)
                     /gene="nanQ"
                     /locus_tag="ACHLAG_003911"
     CDS             complement(7620..8084)
                     /gene="nanQ"
                     /locus_tag="ACHLAG_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFP0657049.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(8081..8956)
                     /gene="nanK"
                     /locus_tag="ACHLAG_003912"
     CDS             complement(8081..8956)
                     /gene="nanK"
                     /locus_tag="ACHLAG_003912"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFP0657050.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(8953..9642)
                     /gene="nanE"
                     /locus_tag="ACHLAG_003913"
     CDS             complement(8953..9642)
                     /gene="nanE"
                     /locus_tag="ACHLAG_003913"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP0657051.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(9690..11180)
                     /gene="nanT"
                     /locus_tag="ACHLAG_003914"
     CDS             complement(9690..11180)
                     /gene="nanT"
                     /locus_tag="ACHLAG_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFP0657052.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(11290..12183)
                     /gene="nanA"
                     /locus_tag="ACHLAG_003915"
     CDS             complement(11290..12183)
                     /gene="nanA"
                     /locus_tag="ACHLAG_003915"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFP0657053.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(12305..13096)
                     /gene="nanR"
                     /locus_tag="ACHLAG_003916"
     CDS             complement(12305..13096)
                     /gene="nanR"
                     /locus_tag="ACHLAG_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFP0657054.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         3172 a         3643 c         3549 g         2899 t
ORIGIN      
        1 tctctaacga cttccctttt agccttaaag ataaaatcca ttttaatttc agtaatttaa
       61 taaagaattt tgcgctaaag cacatttctg taccaataag cttgccattt gacctgtatc
      121 agctttcccg ataagttgga aatccgctgg aagctttctg gatgagcagc ctgctcatca
      181 tatttatgca gtaattgaga tcccctcttc accgtattaa ccgatgcgaa aaggacaaca
      241 agggggcgaa tgcgaggcgc gcgtatgaca cgcaaacccc gtcgccacgc tctttctgtg
      301 cccgtgcgca gcggttcgga agtggggttc ccgcagagcc tgggggaggt tcacgatatg
      361 ttgtacgata aatcccttga gagggataac tgtggtttcg gcctgatcgc ccacatagaa
      421 ggcgaaccta gccacaaggt agtgcgtact gcaatacacg cactggcccg catgcagcac
      481 cgtggcgcga ttctcgccga tggtaaaacc ggcgacggtt gcggcttgct gttacaaaaa
      541 ccggatcgct tttttcgcat cgttgcgcag gagcgcggct ggcgtttagc aaaaaactac
      601 gctgtcggga tgctcttcct gaataaagat cctgaactcg ccgctgccgc acgccgcatc
      661 gttgaagaag agctgcaacg cgaaaccttg tcgattgtgg gctggcgtga tgtccccact
      721 aacgaaggcg tgctgggtga aatcgccctc tcctctctgc cccacattga gcaaattttc
      781 gttaacgctc cggcaggctg gcgtccgcgc gatatggagc gccgtctgtt tatcgcccgc
      841 cgccgcattg aaaagcgtct cgaagccgac aaagacttct acgtctgtag cctgtcgaat
      901 ctggtgaaca tctataaagg tctgtgtatg ccggcggatc tgccgcgctt ttatctggat
      961 cttgcggacc tgcgtctgga atcggccatc tgcctgttcc accagcgctt ctccactaac
     1021 accgtaccgc gctggccgct ggcgcagccg ttccgctacc tggcgcataa cggtgaaatc
     1081 aacaccatca ccggtaaccg ccaatgggcg cgtgcgcgta cctataaatt ccagacgccg
     1141 ctgatccctg atctgcacga cgccgcaccg ttcgtcaacg aaaccggttc tgactccagt
     1201 tcgatggata acatgctgga actgctgctg gcaggcggga tggatatcat ccgcgccatg
     1261 cgtctattag taccacccgc ctggcagaac aacccggata tggacccgga actgcgtgcc
     1321 ttctttgact ttaactccat gcatatggag ccgtgggatg gcccggcggg catcgtgatg
     1381 tccgatggtc gcttcgctgc ctgtaacctc gaccgtaacg gtctgcgtcc ggcacgctac
     1441 gtcattacca aagacaagct catcacctgc gcctctgaag tcggtatctg ggattatcag
     1501 cctgacgaag tggtcgaaaa aggccgcgtc gggccaggcg aactgatggt gatcgacacc
     1561 cgcagtgggc gtattctgca ctcggcagaa accgatgacg atctgaaaag ccgccatcca
     1621 tataaagagt ggatggagaa aaacgtccgc cgactggtac cgtttgaaga tctgcccgat
     1681 gaagaagtgg gtagccgcga actggacgac gacacgcttg ccagctacca gaaacagttt
     1741 aactacagcg cggaagagct ggactccgta attcgcgtac tgggcgaaaa cggtcaggaa
     1801 gcggtcggtt cgatgggcga tgatacccca ttcgccgtgc tctccagtca gccgcgcatt
     1861 atttacgact acttccgcca gcagtttgcc caggtgacta acccgccaat cgacccgctg
     1921 cgtgaagcgc atgttatgtc gctcgccacc agtatcggtc gtgaaatgaa cgtcttttgc
     1981 gaagcagagg gccaggcgca ccgtttaagc tttaaatcgc cgattctgct ctactccgat
     2041 ttcaaacagc tcacgacgat gaaagaggag cactaccgcg cagatacgct ggatatcacc
     2101 tttgacgtca ctaaaaccac gctcgaagcg acagtcaaag agctgtgcga caaagccgaa
     2161 aaaatggtac gtagcggcac cgtgctgctg gtgctctccg accggaatat cgctaaagat
     2221 cgcctgccgg ttccagcccc gatggcggtt ggcgcgatcc agacccgtct ggtcgatcaa
     2281 agcctgcgtt gcgatgccaa catcatcgtc gaaaccgcca gcgcccgcga tccgcaccac
     2341 ttcgccgtgt tgctgggctt cggcgcgacg gctatttatc cataccttgc ctatgaaacg
     2401 ctgggccgcc tggtagacac ccatgcgatt gccaaagatt atcgtaccgt gatgctcaac
     2461 taccgtaacg gcatcaacaa aggcttgtac aaaatcatgt ccaaaatggg catctccacc
     2521 atcgcctctt accgctgctc gaaactgttt gaagcggtcg gtctgcacga tgatgtagtg
     2581 ggcctgtgct tccagggggc ggtcagccgc attggtgggg cgagctttga agacttccag
     2641 caggatctgc tgaacctgtc gaaacgtgcc tggctggcgc gtaagcccat cagccagggc
     2701 ggcctgctga aatacgtcca cggcggcgaa taccacgctt acaacccgga cgtggtgcgc
     2761 acgctgcaac aggcggtaca aagcggcgag tacagcgact atcaggaata cgcgaagctg
     2821 gttaatgagc gtccggcaac cacgctgcgc gatctgctgg caattacgcc gggtgaaaac
     2881 gcggtcaaca ttgctgatgt tgaaccggca agcgaactgt ttaaacgctt tgataccgcc
     2941 gcgatgtcta tcggcgcgtt aagcccggaa gcccacgagg cgctggcgga agcgatgaat
     3001 agcatcggcg gtaactcgaa ctccggtgaa ggcggcgaag acccggcgcg ctatggcact
     3061 aacaaagtgt cgcgcatcaa gcaggtggct tctggtcgct tcggcgttac tccggcgtat
     3121 ctggtcaatg ccgacgtcat tcagattaaa gtcgcccagg gcgcgaagcc gggcgaaggc
     3181 ggtcagttgc cgggcgacaa agtcactcct tacatcgcca aactgcgcta ttcggtgccc
     3241 ggagtgacgc tgatctcccc gccgccgcac cacgatatct actctatcga ggacttagcg
     3301 cagctcattt tcgacctcaa gcaggttaac ccgaaagcga tgatctccgt gaagctggtt
     3361 tccgaaccgg gcgtaggcac catcgcgact ggcgtggcaa aagcttatgc cgatttaatc
     3421 accatcgcag gctatgacgg tggcaccggc gcaagcccgc tgtcgtcagt gaaatacgca
     3481 ggttgcccgt gggagctggg gcttgttgaa acccagcagg cgctggttgc caatggtctg
     3541 cgtcataaga tccgtttgca ggtcgatggc ggcctgaaaa ccggtgttga tatcatcaaa
     3601 gcggcgattc tcggcgcaga aagcttcggc ttcggcactg gcccgatggt ggcgctcggc
     3661 tgtaaatatc tacgtatttg ccatctgaac aactgcgcaa cgggtgtagc aactcaggat
     3721 gacaaactgc gtaagaacca ctatcacggc ctgccgttca aggttaccaa ttactttgaa
     3781 tttattgccc gtgaaacccg cgagctgatg gcacagctgg gcgtaacgcg tctggtggat
     3841 ctgattggtc gcaccgacct gctgaaagaa ctggacggtt tcaccgccaa acagcaaaaa
     3901 ctggcgctgt cgaagctgct ggagaccgcc gaaccgcatc caggtaaggc actctactgc
     3961 accgaaaaca acccaccgtt tgataacggc ctgctgaacg cgcagttgct gcaacaggcg
     4021 aaaccgtttg tcgatgaacg ccagagcaaa accttctggt tcgatattcg caacaccgac
     4081 cgttctgtcg gcgcgtcgct ttcaggctat atcgcccaga cgcacggcga tcaggggctg
     4141 gcagccgatc ctatcaaagc gtacttcaac ggcaccgcag gccagagttt cggtgtgtgg
     4201 aacgcgggcg gcgtggaact gtacctgacc ggggatgcca acgactatgt cggtaaaggc
     4261 atggcgggcg gcttaatcgc cattcgtcct ccggttggtt ccgccttccg cagccatgaa
     4321 gcaagtatta tcggcaacac ctgcctgtat ggcgcgaccg gtggtcgtct gtatgccgca
     4381 ggccgcgcgg gtgaacgttt cggcgtgcgg aactccggtg ctatcaccgt ggtagaaggc
     4441 attggcgaca acggctgtga atatatgacg ggcggtattg tctgtattct gggtaaaacc
     4501 ggcgttaact tcggtgcggg catgaccggc ggcttcgctt acgttctcga tgaaagcggt
     4561 gatttccgca aacgcgttaa cccggaactg gtcgaggtct taagcgttga cgatctggcg
     4621 atccatgaag agcatctgcg cggtcttatc accgagcatg ttcagcatac tggctctcag
     4681 cgcggtgaag agattctggc gaactggtca accttcgcca ctaaatttgc gctggttaaa
     4741 ccgaagtcca gtgatgtaaa agcactgctg ggtcatcgta gtcgtagcgc agcagagttg
     4801 cgcgtgcagg cgcagtaagg ggtagcaaca atgagtcaga atgtttatca atttatcgac
     4861 ctgcagcgcg ttgatccgcc aaagaagccg ctgaagatcc gcaaaattga gtttgttgaa
     4921 atttacgagc cgttttccga aggccaggcc aaagcgcagg ctgaccgctg cctgtcgtgc
     4981 ggcaacccat actgcgagtg gaaatgtccg gtacacaact acatcccgaa ctggctgaag
     5041 ctcgccaacg aggggcgtat ttttgaagcg gcggaactgt cgcaccagac caacaccctg
     5101 ccggaagttt gcggacgagt ctgcccgcaa gaccgtctgt gcgaaggttc ctgcactctg
     5161 aacgatgagt ttggcgcggt gaccatcggc aacattgagc gttatatcaa tgataaagcg
     5221 ttcgaaatgg gctggcgtcc ggatatgtcc ggcgtgaagc aaaccggtaa aaaagtggcg
     5281 attatcggcg caggcccggc aggtctggcg tgtgcggatg tcctgacgcg taacggcgta
     5341 aaagccgttg tcttcgaccg tcatccagaa attggcgggc tgctgacctt cggtattccg
     5401 gccttcaagc tggaaaaaga ggtaatgacg cgtcgccgtg aaatcttcac cggcatgggt
     5461 attgaattca aactcaatac cgaagtgggc cgcgacgtgc agctggacga tctgctgagt
     5521 gattacgatg ccgtcttcct tggcgtcggg acttatcagt caatgcgcgg cgggctggaa
     5581 aacgaagacg ccgatggcgt gtacgcagcg ctgccgttcc ttatcgccaa caccaaacag
     5641 ttaatgggct ttggtgaaac ccgcgacgaa ccgttcgtca gcatggaagg caaacgcgtg
     5701 gtggtccttg gcggtggcga cactgcgatg gactgcgtgc gtacgtccgt gcgccaggga
     5761 gcgaagcacg ttacctgtgc ctatcgtcgt gatgaagaga acatgccggg ttcccgccgc
     5821 gaagtgaaaa acgcgcggga agaaggcgta gagttcaaat tcaacgtcca gccactgggg
     5881 attgaagtga acggtaacgg taaagtcagc ggcgtaaaaa tggtacgtac cgaaatgggc
     5941 gaaccggatg ccaaaggccg tcgccgcgcg gagatcgttg caggttccga acatatcgtt
     6001 ccggcagatg cagtgatcat ggcgtttggt ttccgtccac acaacatgga atggctggca
     6061 aaacacagcg tcgagctgga ttcacaaggc cgcatcatcg ccccggaagg cagcgacaac
     6121 gccttccaga ccagcaaccc gaaaatcttt gctggcggcg atatcgtccg tggttccgat
     6181 ctggtggtaa ccgctattgc cgaaggtcgt aaggcggcag acggtattat gaactggctg
     6241 gaagtttaag ctctgtccca gcgccgcctc ctcagaaaag agtcggcgct gaaattctcc
     6301 tttctttctg gaaagcgcct cgcataatct gaagaaaacc aatgtaatac gctcatctgg
     6361 attgttaccg gtagatgccc ggcgtatagc tgattccatt attcaacgca gcgccatata
     6421 aagggacaga taatggaatc gctctcagag agaacctcaa caggctacca gcaaatccac
     6481 gacggtatta ttcatctggt cgatagcgcc cggacggaaa cggtacgtag cgttaacgcg
     6541 ctgatgaccg cgacatactg ggaaattggc cgacgaattg tcgaatttga acaaggtggc
     6601 gaggccaggg ctgcgtatgg tgcgcagcta atcaagcgac tatcaaagga tttaagtcta
     6661 aggtataagc gtgggttctc tgcaaaaaac ttacgccaaa tgaggctttt ttacctcttt
     6721 tttcaacatg ttgaaattcg ccagacagtg tctggcgaat taacaccatt gggaattccg
     6781 cagacaccat ctgcagaatt cccatcggca aagatttggc agacgctgtc tgccaaatct
     6841 ttccccctgc cctggtccac ctacgtccgt ttactttccg ttaaaaacgc tgacgcccgc
     6901 agcttttatg aaaaagagac gctccgctgt ggctggtctg ttcgccagct agaacggcaa
     6961 attgcgaccc aattttatga gcgaacacta ctgtcacatg acaaatcagc catgctgcaa
     7021 caacacgctc ctaccgagac gcatattctt ccgcaacagg cgatacgcga tccctttgtg
     7081 ctcgaatttc tggaattgaa agatgaatat tcagaatctg atttcgagga ggcgctgatc
     7141 aaccacctga tggatttcat gctggaactt ggggatgatt ttgcctttgt tggtcggcag
     7201 cgaaggttac gcattgatga caactggttt cgtgtcgatc tgctgttttt ccaccgccgt
     7261 ttacgctgcc tactaatcgt cgatctaaaa gtgggcaaat tcagctatag cgatgccgga
     7321 cagatgaata tgtatctcaa ctacgccaaa gagcactgga cgctaccgga tgaaaatccg
     7381 cccatcggtc tggttctctg tgcagagaaa ggagccggag aagcacatta tgctctggca
     7441 ggtttgccta acaccgttct ggcaagcgaa tataagatgc aactacctga tgagaaacga
     7501 ctcgcagatg aactcgttcg aacacaggcg gtgctagagg aaggctatag acgccgttaa
     7561 tttcagcggt ttcgaattct gcagacagtg tctgcagaat ctttaaaacg aactaagttt
     7621 taagccatta aatcagcctt aaccttcacc acaacctttt taatctctcc aggctcgccg
     7681 acaacgcatc ctggtttatg cggttcacct ggcataaaca cggcgaacat tcccggtttt
     7741 aagatgatgg cttgctcgtt atcaatggtg ctgcaaagct gataatcatc ctcatggtgg
     7801 aactcttcac actggcgcgc agtgcctgcc atgccaaaca gaatccgttc ctcaccgttt
     7861 aataacagtt ggatatcaat gtattgctcg tgcaattccg cttttttctc gacgggcgat
     7921 tgagtgttaa acgtcatgac attcataaag atattgtcgc cctgtaattc gtaacgaccc
     7981 ggcgcttttt cttgcggtct ggcagctaat gccagcgtta acgcgtcctg taacgcagga
     8041 tgtaacccag cagacggtaa tgactgtact tcacccatca tcataatttt tctccctggg
     8101 ccaacagcgc agccccaagt aaacctgcat cgtggcggta gtgcgccgcc agtaaatcaa
     8161 catgaaatgc cgctggctcc tgcgccagat acgtttccac cagcgccaga tacccttctg
     8221 ccagcccaac gctgccaccg accaccacgc actggcaatc agttgtggct ttaatatcag
     8281 cgatcagcct tgcaagcgta cgtgcggagc ggtgaatcag ctgctgcgcc tgctcgtcgc
     8341 cctgcccggc gcgtgtgaaa atagttctcg catccgcgcc cgccagttca ccctgcgctg
     8401 ccgccgcaat gccgcgacca gaagcaatcg cctccacgca acctgtgcgt ccacagccac
     8461 agaccgggcc gtgtggatcg gcaagcgtat gcccgatgtg ccccgccaga ccgccagggc
     8521 cggtaagcag tttgccgccg ctcactacac cgccgccgac gccagtggaa acggtgataa
     8581 agaccatatc ggttatatcg ccttccagcg cctgatactc tgcccatgct gcggcctgcg
     8641 cgtcgttaat ggcaatggtc ggcaaattgg taagttgttc cagcgttttg actaacggaa
     8701 agtgtagcaa tccaccgaga ttatgcggat tcagcgccag caaactgccg tcgcgaataa
     8761 ttccggttga agcgatggca acccgctgcg catgagcttg caacggagag actaatgcgg
     8821 ataaggcatc acgcaaggct tctggtgtct ggctggctgg cgtaggaagt tcacgacgat
     8881 cgcggatctg cccgtcagcg ccaatcagcg cggcggcaag tttagtaccg ccgatatcaa
     8941 tcgccagcgt ggtcatagca ccgccttttt catcgctgtg ttgtaccact gacaaatgtg
     9001 ttcaagacgc gtaatggcag aaccgaccgt caccgcccac gcgccgtggc gcatcgcatc
     9061 tgccgcctga gcaggcgtgt tgtaacgccc ttcggcaatc accctacatc cggcgtcgct
     9121 caacgttttc accagcgcca gatccggctc ttctggcgtt tcaggcgtgg tatagccaga
     9181 aagcgtagtg ccaataattt cggctcccag cttttggcat gccaggccgt cttccggcgt
     9241 tgagcagtcg gtcatcgcca gtaaaccgtg atggtgaata cgtgccagca gcgtttcaac
     9301 aggcaccgga cgcgggcggt cggtgccgtc aatggcgata atgtccgcgc ccgcctgcgc
     9361 cagcgcatca acatcttcaa tataggccgt gatgcgtacc ggagaatcct ccagatcgcg
     9421 tttcacaatt ccaataatcg gcacgctcac caccgcacgc gtggcttgca gatttgccac
     9481 accttcaatg cgaatggcaa ccgcgcccgc ctgttctgcc gctaatgcca tggcggcgac
     9541 gatttcgggt ttatcgagcg ggctgtccgg aaccggctgg caggagacaa tcaggccacc
     9601 gttagcagcg attttttgat ccagttgtgc aagtaacgac atacatcttc ccttagcgaa
     9661 aggcccggta catagaccgg gcaacaggat taacttttgg ttttgactaa atcgtttttg
     9721 gcgctgccaa atggcacggc accgctaaat ggtttgccgt cgatagcgtc atgagtacgc
     9781 aatgcttccg ggcgcagcca acgctgaacg cgggaaggca tatccagacc aatcagcagg
     9841 atcaccacaa atgtcagact gaacgatagc gatgccagcg cagtacccag atccagacgt
     9901 tgagcgatca acgcgccgat gattggggcc agtgcgccgc ccaatgcgcc aacgttgtag
     9961 gtaaagccca ggcccgctgc acgctggtcg gtatcgaaat aaccgccaat cagtttcggt
    10021 aagatcccgg cgatcccttg tccaagcatt tgctggaaga acagtaacag accgagcacc
    10081 cagacgtttg cgccgccaat cgcaaatacc ggaataatca acagctgcga ggccaacagg
    10141 ctacaaacgt acgctttgcg ggttcccagc cagtcaccga ggaagccgcc gacacagcac
    10201 cccaccgcag ccccaaagcc gctaaagaac agcacattgg cgacagtatg cgggttataa
    10261 gccagatcgg ttttcagata cgttggcagc agcgcctgaa tcggccatga gtagaggaaa
    10321 gcaaacaaca cgaccaccat cagcattacg cccgttggcc agcgtttacc cgtactttgc
    10381 accataaagc tgataaagat tgcggcgcat aacagcccaa gaacagcgac gatcgcggca
    10441 ttttgcaggt taccggcgaa gcaaaaccac agcgcagtag ccgccgccag tgtcattacg
    10501 atattggcaa tgcgatgttc accacggtag agaatatcta ccatcgtgcg tactggtgct
    10561 ttacctgcat gtttctcttt ccagtcttcc gcttccggga tatttttacg cagccagaga
    10621 gcaaagatga taggcaaaat gccgataaag aacagcgcac gccagcccca gaccggaacc
    10681 accaggctat agacctgagc ggcaacgacg gcccccacag agaagcctga aatcagaaaa
    10741 ccactggctt tgttacgcag atgttttggc cagctttcaa tgacataggt ggcgctggaa
    10801 ccgtattcac ccgccatccc catgccgatg accagacgcg cgataaacat ggtgatgtag
    10861 cctggcgcaa agccgcaggc cagcgtcccg gccgagaaga gaacgatgct ggtgaccatt
    10921 gccagacgac gcccgtagcg gtcacccata gcgccgagca tcaggccgcc gaaccagcga
    10981 gagataaagg ctgcggagat cagacttgcc gcctgcaccg tcgtcagccc gaattcacct
    11041 tgtacttcgg tgagtaccag ggcgattaaa acgaaatcaa aaccgtcaag cagatatccc
    11101 aaccaggcag cggaaaatgc ccgccattgt gcacgattga gatggcgata ccacgggata
    11161 ttctgggttg tagtactcat tgtgagtctc ccacggtggg cgatgcccac acgctttggt
    11221 atgaaaattg tagggtacag atgcgtttat ttcccctcac ccggtagggg cgagcgaggg
    11281 gaaaacaact cacccgcgct cttgcatcaa ctgctgggcc agcgccttca gttctggcag
    11341 atatttttca tctaccggtc caaacggttt gcggcacagc ggcacagaaa cgacatccat
    11401 ataatggagg acagttttca ggccgcggaa tacgcccgtt ttgatcagta aatcaatgac
    11461 tttattgcat tcagtttgca gtttctgcgc ggtctggata tcgccttctt tcagcgcctt
    11521 aacgatcccc tgatagcgcc agcccatgat gttgtaggta ctaccgatac caccatcagc
    11581 gcccgccagc agaccagagg cgaagatttc gtcgtaaccg ttatagagca caagatcagg
    11641 atgttcacga cggatctgct ccatctgata gagatcgcca gaagtctgtt tcagcgcacc
    11701 gacgccaggc aacgtaacca gggtgttgat ctgatccagg gtcagtttta ccccactcag
    11761 ggctggaatg ttgtacacca ccatcggcaa accatccgct gaatcaataa ttgcccgata
    11821 gtgatcgcag tgttcttcaa agctgaaagg atagtagaac ggcgtgacgg cggagacggc
    11881 atcgaagcca taacgtttag ccgatgccgc aagttgttgg ctttcggcgg tgctgacgca
    11941 accgacgtgg gcgatgagtt taatcttacc tttcgcctct tcggcgacga tttccagtac
    12001 ctgttcacgc tcggaaaggc tttgtacaaa ggcctcgccg gtcgaaccac ccacgtataa
    12061 accgtcgatg ccctgctgaa tattgaactg aaccaggcga cgcagactcg ctttatccag
    12121 tgcttgttgt tgatcaaaag gagtcaggag tgcagccatt acgccacgta aattcgttgc
    12181 cataaatacc tctgaagtga tgcttgtctg ataaacgata tacctttata cctgttatac
    12241 cagatcaatt aagcaacacc ccatacagaa agcttataat gcgatctgct tcactaaagt
    12301 ggcattattt ctttttgttg gtggtctgac cgaaagcgtg ccaggtagca gagacgctgt
    12361 tgagatgcga ttgcaacgca cgatcggctt cgtcaggatc atgacggcgg atcgcatcaa
    12421 cgatcgcaat atgctgttga taactaacgt tgttatgttc gtgcagtgcc tgatcggtaa
    12481 ccgttgggcg tgcggcaata agccagtcga gcagggcaac gtggatcgcc atgaagattg
    12541 ggttaccggg gatctccgcc agcacgcggt ggaaatcaac gtctgaacga atgaatgccg
    12601 cgttgttatc cagcgactga ctgttaattt ccagtgcttt tgccagcaaa tcgatttgct
    12661 catcggtggc atgttcagcc gcatagcgca ccaggctgga ttcaaagaac agacgtaatt
    12721 gttcgaaatg ggcaatccca ccaggatgag aaaggaaatc tttcgccatt ccggaaagct
    12781 cgccgatgat ggtgtccgca gaaggacgtg agacgcgggc acgttcgccg ttgttgattt
    12841 gcaccagacc tttgcgtttt aacgctgcca gcgcttcgcg cacagaagga cgcccgacgt
    12901 taaagaacgc catcagttcg cgttcagacg gtaattgttc accttcgcca aattcacgac
    12961 ggcggatcat ctgttccagc tcttcttcca ccatttcgga gagtttttta cgcgccagcg
    13021 ggcggctacg caagttgcga ccaattgcag gtgaagaatc ttcggtttgc gaatcaaatg
    13081 cgttcataag gcccattctg taaggtcagt gtgattaaca tcatcagtga catcctatca
    13141 caggattgaa agtaggggaa aatggcaggt ttttctcttt gtgcctcatc attaccataa
    13201 ttaacggaat aattaactat tgcgaaaaat taatgtaacg cagataaaaa catcccgttt
    13261 gaa
//
LOCUS       DAZGVQ010000136        13186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000136 DAZGVQ010000000
VERSION     DAZGVQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.137"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..216)
                     /locus_tag="ACHLAG_003917"
     CDS             complement(<1..216)
                     /locus_tag="ACHLAG_003917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657055.1"
                     /translation="MPEQAITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEI"
     gene            153..1652
                     /gene="pitB"
                     /locus_tag="ACHLAG_003918"
     CDS             153..1652
                     /gene="pitB"
                     /locus_tag="ACHLAG_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFP0657056.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGMQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1701..2393)
                     /gene="yghT"
                     /locus_tag="ACHLAG_003919"
     CDS             complement(1701..2393)
                     /gene="yghT"
                     /locus_tag="ACHLAG_003919"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP0657057.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2567..3280
                     /gene="yghS"
                     /locus_tag="ACHLAG_003920"
     CDS             2567..3280
                     /gene="yghS"
                     /locus_tag="ACHLAG_003920"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP0657058.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPA
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            3312..4070
                     /locus_tag="ACHLAG_003921"
     CDS             3312..4070
                     /locus_tag="ACHLAG_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657059.1"
                     /translation="MDALQTQTVISTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4116..5465
                     /locus_tag="ACHLAG_003922"
     CDS             4116..5465
                     /locus_tag="ACHLAG_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFP0657060.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVFI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5465..6289
                     /locus_tag="ACHLAG_003923"
     CDS             5465..6289
                     /locus_tag="ACHLAG_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657061.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            6301..6861
                     /locus_tag="ACHLAG_003924"
     CDS             6301..6861
                     /locus_tag="ACHLAG_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFP0657062.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6892..7962
                     /gene="lptF"
                     /locus_tag="ACHLAG_003925"
     CDS             6892..7962
                     /gene="lptF"
                     /locus_tag="ACHLAG_003925"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFP0657063.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7959..9038
                     /gene="lptG"
                     /locus_tag="ACHLAG_003926"
     CDS             7959..9038
                     /gene="lptG"
                     /locus_tag="ACHLAG_003926"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFP0657064.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMNLEELSVPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9074..10246)
                     /locus_tag="ACHLAG_003927"
     CDS             complement(9074..10246)
                     /locus_tag="ACHLAG_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP0657065.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIAEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFK
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10246..10494)
                     /locus_tag="ACHLAG_003928"
     CDS             complement(10246..10494)
                     /locus_tag="ACHLAG_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP0657066.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10526..11440)
                     /locus_tag="ACHLAG_003929"
     CDS             complement(10526..11440)
                     /locus_tag="ACHLAG_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP0657067.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTHCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVPGGYDW
                     QHVRQLAADARCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEQALRNGLF"
     gene            complement(11437..13125)
                     /locus_tag="ACHLAG_003930"
     CDS             complement(11437..13125)
                     /locus_tag="ACHLAG_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFP0657068.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKAR
                     AEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLTPVSTQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLHVQESLA"
BASE COUNT         3155 a         3268 c         3423 g         3340 t
ORIGIN      
        1 tatttctaaa ggttttgaat gaatgcttat tgtctgatac acaagaaata acactctttt
       61 tatcgttaaa aaatgatatt tcactttgcc catgccgtta aagtcactga taatgcgtcc
      121 gttcgtaaat tcaaaatggc gtaatctaat atatgctaaa tttatttgtt ggccttgata
      181 tatacacagg gcttttgtta ttgcttgctc tggcatttgt gttgttctac gaagcaatca
      241 atggttttca tgacacggcg aatgcggtgg caaccgttat ttatactcgt gccatgcaac
      301 cacaacttgc tgtggtgatg gcggcatttt ttaacttttt tggcgtgtta ttgggcggac
      361 ttagcgttgc ctatgccatt gtccatatgt tgccaaccga tttgttgctg aatatggggt
      421 caacccacgg cctggcgatg gtcttttcca tgctgctggc ggcgattatc tggaacctgg
      481 gaacgtggtt cttcggttta ccggcctcca gttcgcacac cttgattggt gcgattatcg
      541 gcatcggttt aaccaacgcg ctgttaaccg gctcatcggt gatggatgcg ttaaacctgc
      601 gtgaagtgac caaaattttc tcctcgctga ttgtttcccc tatcgtcggc ctggtcattg
      661 cgggaggcct gatattcctg ctgcgacgct actggagcgg gacgaaaaag cgtgaccgta
      721 ttcaccgcat tccggaagat cgcaaaaaga aaaaaggcaa acgtaaaccg ccattctgga
      781 cgcgtattgc gctgattgtt tccgctgcgg gcgtggcgtt ttcgcacggc gcgaacgacg
      841 gacaaaaagg gatcggcctg gtgatgctgg tactggtggg gattgcccct gctggcttcg
      901 tcgtcaatat gaacgcgtcc ggctatgaaa ttacccgtac ccgcgatgcc gtcaccaact
      961 tcgaacacta cctgcaacag catcctgaac tgccgcagaa gttgattgcg atggaacctc
     1021 cattgcctgc agcatcgact gatggcatgc aagtaacaga gtttcactgt catccggcaa
     1081 atacctttga tgcgattgcg cgcgttaaaa cgatgctgcc aggcaatatg gaaagttacg
     1141 agccgttaag cgtgagtcag cgcagccagc tgcgccgcat tatgctgtgc atctctgata
     1201 cctccgcgaa gctggcgaaa ctgccaggcg tcagtaaaga agaccagaac ctgctgaaaa
     1261 aactgcgcag cgatatgtta agcaccattg agtacgctcc ggtgtggatc atcatggcgg
     1321 tagcactggc gctcggcatt ggcaccatga ttggctggcg tcgtgtagcg atgaccatcg
     1381 gtgagaagat tggtaagcgc ggcatgacgt atgcgcaagg catggcggca caaatgacgg
     1441 cggcagtgtc tatcggtctt gccagttata ttgggatgcc cgtctccaca acacacgtcc
     1501 tctcgtctgc agttgcaggg acgatggtgg tggacggcgg tgggttacag cgtaaaacgg
     1561 taaccagcat cctgatggcg tgggtattta ctttaccggc ggcaattttt ctttctggtg
     1621 ggctgtactg gatagcattg cagttgattt aaccttcctg aaaatgcccg gtcctggcga
     1681 tcgggcattt ccatttttga ctagtgataa ccacgcgcgg tcataaaatc cgtaatcgct
     1741 ttttctgcat caaccaacac ctgttccagt ggctgattgg catcgatatc aaccagttgt
     1801 gcaccaccaa aggttaactg tggcgttatg gcaatcttcc tcgccagcga ttcccgttta
     1861 tggtcgggtt tacgtgcaca ggcaacttca aggtcaacat tgagtttgat gaccagatca
     1921 ggcttatggc tcgccatcca gtgaaacgct ttacgttcct ggcttgccag ccatgagaca
     1981 aaacgaccac cttcaacgtt aggtgggaac accgtaccat cgtaagcgcc aggaatttgg
     2041 tcctgaggat aacggtcggt tagaacaatt aacccgcgac gacgacaggc aagcatatga
     2101 cgaaagcgca gtaagcgacg ggcgacaaac gctgtaatta ccagcgccgg aactggtcca
     2161 ggcaattttt ttgctgtttt cacctgattt cgttcaattg ttttatgtaa ggattttccc
     2221 atcaacggta attttgtcac tgcacgaccg acatttccgg cctgttttcc taaatgaact
     2281 ctttcggcag caccatattt ttcgacaacg gtaatgagat gttcacacac cgttgacttg
     2341 cctgaaccat cgctaccaat aacggcaatt aatggaggtg ttattgactg catgaatata
     2401 tccttaaatt taaatatcca tccaaaatat ttatttggtt aatatgtttt tatgaaagcg
     2461 taattcaggt caatgtcaca attaaccatt gtcacaataa ggttgaacgg atattctgag
     2521 cacggaccac gtaatattca atacattatt tactgccggg tttttcgtga atcaacgtaa
     2581 tatgtcaata attaattcca cgccagtgcg tgttattgcc attgtaggat gtgatggttc
     2641 aggtaaatcg accctcacgg caagcctggt aaatgaactg gcagcaagaa tgccagcaga
     2701 acacatttat ctcgggcaat cgtccgggcg aattggcgaa tggatttcac agctccctgt
     2761 tattggcgca ccttttgggc gttatttgcg aagtaaagcg gcacatgtgc acgaaaagcc
     2821 ctcaacaccg cctggcaata ttactgctct ggttatctat ctgctttcct gctggcgggc
     2881 gtacaagttt cgcaaaatgt tgtgtaaaag ccagcaaggc tatctgctca tcaccgaccg
     2941 ctacccgcag gttgaagtgc cgggttttcg ctttgatggc ccgcaattgg caaaaaccac
     3001 gggcggtaac ggttggataa aaatgttaag gcagcgcgag ctgaagctgt accaatggat
     3061 ggcatcttat ttgcccgtat tgttgattcg tcttggcatt gatgaacaaa cggcgtttgc
     3121 gcgtaaacct gaccaccaac tggcagcgtt acaggaaaaa atcgccgtta cgccgcaact
     3181 gactttcaac ggcgccagga tccttgaact ggatggtcgg caaccagccg atgaaattct
     3241 gcaagcgtca ctacgcgcaa ttcacgccgc gctttcctga tcaccaaaat ggatagggga
     3301 tgatttaacg aatggatgca ctacaaacac aaactgttat tagtacaacc gcgccgcagc
     3361 ccagctacat tccggggcta attgcggtgg tcgggtgtga tggcaccggt aaatccacac
     3421 tgaccaccga cctggtgaaa tcgctgcaac aacactggca aaccgagcgg cgctatctgg
     3481 ggctgctctc cggcgaagac ggcgacaaaa tcaaacgatt gccgttggtt ggcgtctggc
     3541 tggaacggcg actggcggcc aaatcctcga aaacccaaag catgaaaacc aaatctccgg
     3601 cgctatgggc ggcggtgatt atgtactgct tctcgctgcg aagaatggcg aatctacgca
     3661 aggttcagcg actggcgcaa agtggcgttc tggtggtcag cgatcgcttc ccgcaggctg
     3721 aaatttcggg cttttattat gacggaccgg ggattggcgt cgaacgtgcg accgggaaaa
     3781 tcagcatgtt tctggcgcag cgcgaacggc gtttatacca acaaatggcg caatatcgcc
     3841 cggaattaat tattcgcctg ggcattgata ttgagactgc catctcccgc aagcctgacc
     3901 atgactatgc cgagctgcag gacaaaatcg gtgtcatgtc gaagattggc tataacggca
     3961 caaaaattct tgagattgat tccagagcgc cctacagcga agtgctggaa caggcacaga
     4021 aggcggtttc tctggttgcc atcgtttctg accgccgaag tttaacttaa ggctggcggg
     4081 atacttttta ctatcaacga taaggtcagg ctgaattggc gggttttaac atcaaacatt
     4141 ggtttgcaga tggcgcgttt cgcaccatta ttcgcaatag cgcctggtta ggctccagta
     4201 atgtcgtgag cgccttgctg gggctgttgg cgctctcgtg tgccggtaaa gggatgacgc
     4261 ccgccatgtt tggcgtactg gtgattgtgc aatcgtacgc caagtcgatc agcgatttta
     4321 ttaagtttca gacatggcaa ctggtggttc agtacggaac accagcatta accaacaata
     4381 atccgcagca attccgcaat gtcgtctcat tttccttctc gctggatatc gtcagcggcg
     4441 cggtggcgat tgtcggtggc attgccttac tgccattcct ttcccattca ttaggtctgg
     4501 atgaccaaag tttttggctg gcagcgctct attgcacgct cattccttca atggcttcct
     4561 ccacgccgac cggcattctg cgtgcggtag atcgcttcga tttaattgct gtacagcagg
     4621 cgacgaaacc ttttctgcgc gcagcgggga gcgtcgtagc ctggtatttt gactttggtt
     4681 ttgcgggttt tgttattgcc tggtacgtgt cgaatctggt tggcggcacc atgtactggt
     4741 ggtttgccgc gcgcgaatta cgccgccgaa atatccataa tgccttcaaa ttgaatctgt
     4801 ttgagtctgc ccgacacatc aaaggcgcgt ggagttttgt ctggtcaacc aacattgccc
     4861 actccatctg gtcggcgcgt aactcgtgca gcacagtgct ggtggggatc gtgttaggac
     4921 ccgctgccgc cgggttattt aaaatcgcca tgacattctt cgacgccgcc ggaacgccag
     4981 cgggtttgct gggtaaaagt ttttacccag aggtgatgcg tttagatccg cgcaccacca
     5041 gaccgtggtt gttaggtgtg aagtccggtt tactggcggg cggaatcggt attctggtgg
     5101 cgctcgcggt gtttatcgtc ggcaagccgc tcatttcgct ggtgtttggc gttaagtatc
     5161 tcgaagcgta tgacctgatt caggtgatgc tgggcgcaat tgtgatttcg atgctgggct
     5221 tccctcagga gtcattgcta ttgatggcgg gaaaacagcg cgcttttctc gtggcgcaaa
     5281 ccatcgcctc aatcggctac atcgtattgc tgttcatgtt ctgtcatctg tttggtgtgc
     5341 tgggagcagc gtttgcttac ttcggtgggc aatgtctgga cgtggcgctc tcgttaattc
     5401 caaccttaaa ggcatttttt cagcgccatt ccttgcttta taacgcagcc ggagagaaat
     5461 cctgatgatg agaaaatatt tcccgcttga ggcgagcgag cgtctatttg ttgcgattga
     5521 agaagatgat gtcgttgatg cacaggttag tttgccgccg actattgcac taagttgtac
     5581 caccgaaatc attcatgata attacgcgtt atgcctgcaa ttctggctca atggcgttga
     5641 tcgtcaggaa ttactgcgtt tagttcgcaa acaagccaaa ggcgatgagc tgactgctga
     5701 tgagcgcaaa caatttaaat acatgcgagc gcgctacaag cacctgcgtt ttgcgcaacg
     5761 gctgtattta aagaagcatc aggccggatt tttattcggc aaaacgaccg tttttcttgg
     5821 gcgttttcag gacggctttc gtaacggtaa gaaaaatatt gtgtcgtatt acggcaacct
     5881 gttacggatt tatttaagct ctccggtctg gtcgttggtg aactattcat tacgccatag
     5941 ccagttagaa agcgtaagca gttttattgc ctatcggcaa aagcaaatgc ataccctgaa
     6001 agagattatt gccaaaccgc gactaaccgg cagggaattt catgatgtgc gaaaaatcat
     6061 cagccaacag gtttcttatt acgacacctt aagatcgctt gatcctgaaa ataaagaggc
     6121 gttacagatt tcccgtttcc tcgccgccat taatggcctg atgggagata aacacgacga
     6181 catggtggcg gatgatatgg aaaaccgcca gtcgtatgat gcaccgttgg cgctggatag
     6241 cgatattcgt cagcgcttag agctgctgat ttcgcgtttt ccgctataac cggagcaatg
     6301 atgtcatctc gtttgattat tgcgctgatt atcatgcttt tagccccggg tgtgcaggca
     6361 cacaatttcg tcactggcaa aacggttacc cctgtttata ttcaggaagg cggcgagctg
     6421 cttctgaata gcgacgacga aattcactac caaaagtgga acagcacaca attggcggga
     6481 aaagtccgca ttatccagta tatcgccggg cgtaagtcgg cgaagaaaaa gaactcactg
     6541 ttaattaaag cggttgaagc ggcaaacttt ccgcaggatc gttttcagcc caccaccatt
     6601 gtgaacaccg atgatgccat ctttggtact ggctattttg ttgttggcaa aatcgaaaaa
     6661 aataaacgcc gctatccgtg ggcgcaattc gttattgacg gcaatggaca gggacgcgtt
     6721 gcctggcgct tgccggagca gagttcaacc attctggtgc tgaataaagc cgggcaaatt
     6781 cagtgggcaa aggacggcag cctgacgccg gaagaggtag atcatgtcat tgcgttggcg
     6841 cagaaactga tcaatgaata aaaaactcaa aggattgagg gcgttagtga gatgaagctg
     6901 gttgaacact acatcatgcg tggtacgcgc cgtctggtgc tgattatcgt cggttttctg
     6961 atctttattt tcgccagcta ctccgcacag cgttatctga ccgaagcggc aaatgggaca
     7021 ttagctcttg atgttgtgct ggatatcgtt ttctacaaag tactgattgc actggagatg
     7081 ttgttacctg ttggtctgta tgtgtcagtt ggcgtgacgc tagggcagat gtataccgac
     7141 tcggaaatta ccgctatctc tgcggcgggg ggcagtccgg gacgcttgta caaagccgtt
     7201 ctttatctgg cgataccgct aagtattttt gtcacccttc tgtcgatgta tggtcgtccg
     7261 tgggcttatg cgcagattta tcaactggag cagcagtcgc agtcggagct ggatgttcgc
     7321 cagttgcggg caaagaaatt taacactaac gataacggac gaatgatcct ttcgcagacg
     7381 gttgatcagg ataataatcg cctgactgac gcgctgattt atacttctac tgccaatcga
     7441 acccgtattt tccgcgcccg ttcggttgat gtggttgacc catcacccga gaaaccgacc
     7501 gttatgttgc acaacggcac tgcctatctt ctcgatcatc aggggcgtga cgacaacgaa
     7561 cagatctacc gtaatctgca attacatctg aatccgctgg atcaaagccc taacgtcaaa
     7621 cgcaaagcaa aatcggtcac ggagctggcg cgctccgcct ttcctgccga tcatgccgaa
     7681 ctgcaatggc gacaaagccg tggcctgacc gcattgttga tggcgctgct ggccatttca
     7741 ttaagtcggg taaaaccgcg gcaagggcga ttttcaacgt tattgccact gacgttgctg
     7801 tttattgcca ttttttatgg cggcgacgtc tgccgtacgc tggtggctaa cggcgcgatt
     7861 cctctcattc ctggtttgtg gttagtgccc ggactcatgc taatgggcct gctgatgctg
     7921 gtcgcacgcg acttctcttt gctgcagaaa ttttcccgat gaatgttttc agtcgctatt
     7981 taatccgtca tctctttctc ggttttgccg ccgccgcagg gctattgctg ccgcttttta
     8041 ccaccttcaa cctgattaac gaactggatg atgtcagccc gggcggttat cgctggactc
     8101 aggcggtgct ggtggtgcta atgaccttac cgcgcacact ggtcgaactt tcgccgttta
     8161 tcgccttatt gggagggatt gttggcctgg ggcagttatc gaaaaacagt gagcttaccg
     8221 ccattcgcag cacggggttt tctatcttcc gtattgcact ggtggcgctg gttgccggga
     8281 tattgtggac tgtttcgtta ggcgcgatag atgagtgggt ggcgtcgcca ttgcagcaac
     8341 aggcgctgca aatcaaatcg accgccaccg cgttggggga ggacgatgac attaccggca
     8401 atatgctgtg ggccaggcgc ggcaatgaat ttgtgacggt gaaatcgctg aatgaacagg
     8461 gccagcctgt gggcgtggag atttttcatt atcgtgacga tctttcgctc gaatcctaca
     8521 tttatgcacg cagtgccacc attaaagacg acaaaacgtg gatcctgcat ggtgtgaatc
     8581 ataaaaaatg gcttaacggt aaagaaacgc tggaaacatc agataatctt gcctggcaat
     8641 cggccttcac cagtatgaat cttgaagagt tatcagtgcc ggggaatact ttttctgtcc
     8701 gtcagcttaa tcattacatc cattatttgc aggaaaccgg gcaacccagc agcgaatacc
     8761 gccttgcact gtgggaaaaa ctggggcaac ccatcctgac cctggcgatg attttgctgg
     8821 ctgtgccgtt tacctttact gccccgcgtt cgcctgggat gggtagccgt ctcgctgtag
     8881 gcgtcatcgt gggcttactc acctggatca gctatcaaat catggttaat ctgggattgc
     8941 tatttgcgtt aagcgcacct gttaccgcgc tcggtttacc ggtagcgttt gtgttggtgg
     9001 cgttgagcct ggtgtattgg tatgacagac aacattaaac ccgccgtggc gcaaacacca
     9061 cggcgggctc acattactgc ttcagagtag caaatgcctg aatgatggca tcgatttgct
     9121 cacgactgtg ggcagcattt acgctacagc gcagcagcgt gatgcctgca ggcgcggcag
     9181 gcggcaggat aagattcaca tacacgccca gcgagataag ctcgcgccag atgcgtaaac
     9241 cttcctcttt cgaaccgatt attactggca ctaccgggct aatatgtgaa cttaactcat
     9301 aacccagttt ttgcagaccg tcatacagat ggttggcgtt atccatcagc ttttgccgca
     9361 gctctggttg tgtggcaata gttcttaacg acgagcgcac ggtcgcaatg caggacggag
     9421 aaggagaggc ggtaaaaata tacgggcggc tgccgtagcg cagcacttcc atcgcttccg
     9481 aaccgacagc aaaaccgcca atcgaggcca gacttttact gaaggtgcca acgataatat
     9541 ccacatcatc ttcaacgccg acagcttcag ccagtccgcg ccccgttgcg cctaacacgc
     9601 caaacgaatg cgcttcatcg acaatcaaat aaccaccgag acgacgtttg atatcaacaa
     9661 tttccgccag cggcgcaacg tcaccgagca tgctgtagat gccctcaacg atgataatcg
     9721 cctctttcgc ccgttcaccg aggcgaacca tgcgccgttc cagatcttta gcatcgttat
     9781 ggcggaaacg aataatttcc gcgccaccga gagaacaggc gtcataaata ctggcgtggc
     9841 tgtcggcatc cagtaacacc acggcgttat ggtcggcaag tgcgctgatg acgcctaaat
     9901 tagcggtata accggttgaa aaaacgatag cggtagggcg cttgaaaaac tcggcaatct
     9961 ctttctctaa cgccaggtgc ggcgcataac tgccgtttgc catacgagaa ccggtggtgc
    10021 cggtgccttg ggccgctaac gctgcctgac cttcggcgat ggcatcatga ttaaaggtta
    10081 aaccgaggta attattggtc ccggccagaa tcactttttt attgccgatc cggccttcgg
    10141 tggcggaata cacttcatca atacaggtgc caaacggtgt taacccgttg tcactaaatt
    10201 gcagtcgctc gccagcaagg cgcgcatatt tatcgtatag ccccattatt tattctccag
    10261 ccatggaagt agagtttcca ttaattgcgc aggggttttg acatccagca gaatattaat
    10321 aggaatggaa atatcgaatc tgtcttccag catcattaag agatccatca ctttgatcga
    10381 ctccaggcca agatcgttga cgagatcgct gtcgggctta atttccacac cgttttcgac
    10441 caaatcctgt aaacaggaaa ggatgtaatc cattactatt tcacgattta ccatagaaac
    10501 gttaacacac ctttaaacaa gacaatcaaa atagcccgtt gcgtaatgct tgttccaggc
    10561 taatcccggg aaaccaatta atatcttcag aaatacggtt attacttgcc gaccagtcgg
    10621 cgtgggttaa ttcacgaatt ttcgagcggg tcagcatagg ttctttacct gccaggcgac
    10681 tcaacgcggt actgatatcc gcaaggcagg tgagtaccgg cagaggaata ccaaccattc
    10741 gcacggaacc acaacgggcg tcggcggcaa gctgtcgtac gtgttgccag tcatagccgc
    10801 cggggacgcc atcgcataat tcataggttt gcgtctgtac agtttcggcg cttaaccact
    10861 gacccactgc ttgcgcgaaa tcggtgacgt gcaggaaaga gagctgtgta tctggcgcac
    10921 caagtcgtgg cagcaggccg cgcagcatcc agtcaaacag cggttttaac tctttatcgc
    10981 cgggaccata cacggctgtc gggcgaaaaa cgcccagcgt aatttcgtca gccattgcag
    11041 tcagacgttg ttcggcgacg tgtttggaat ttgcgtacca ggagagctcg ggatggcgcg
    11101 ccgccagcga agagataaac agaaaacgtt ggcaaaagcc gctctctttt gctgcttgca
    11161 tcaggcgcag gctgccgtca acgttacagt gggtaaaaat ctcttctttg tgtccgcgca
    11221 cttgcccggc gcaatggacc accgcgctgg ctccggcaac cagttcgcta agcgaatgtg
    11281 tatcttccag cgaaccgcgc acccaagtaa gattatcatt gacgtgagcg cgggcagtac
    11341 gcgtcaatgc gcgaacgtga aagccgcggg cgagcaggtt atcgataata tatttaccga
    11401 taaacccggt agcgcccgtc accgcgacag tttgattcat gccagggatt cctgcacatg
    11461 aaggcttgca gcataagcct tctgataacg ttttttcgct tccgcacggg caggcttgcc
    11521 ggaggacgtt cggggaatgc tgtggggcgg caacagatcg atagccgcgg ttacgccaaa
    11581 ttcgctttgg atccgtgctg ccagcgcgtg gataagctgt ccgcgacgtt cttcatcgct
    11641 gatccgacac tggatctgca aaatgatttt ttcctgggcg gtaacaaaag caatcgcatc
    11701 gccagaatga atttccggtt cctgttccgc tatatattca atatcctgcg gccagatatt
    11761 acggccacga ataataatca gatctttaat gcgtccggtg acatacagat aaccgtccag
    11821 cagataaccg aggtcgccgg tgtctaacca gcccgtcgcg gcaatctcgt cttgcgaagc
    11881 ctggtcgcca aagtaaccgc tcatcagact ggggccggag atgcaaatat ggcctaccac
    11941 acgttccgcg accggcatac ctgcttcatt gcggatttca ataccatgtt ccggcaacgc
    12001 tttgccgcag ttgacgaaag tcgatacggc gcgtgtctct gcacccggcg cgacggcttt
    12061 accctgatat tcgaggatgt cgcgatccac ttcgttaacc acaaccccgg aggcttcatc
    12121 agagaagctg acagccagcg cattttctgc cagtccgtag cacggcatga aagttttatt
    12181 gtcaaagtta acctgacgga aacattcagc gaattgatgg agttgttctg cggagatggg
    12241 ttctgcacca ataccagcga cgcgccagca ggaaagatcc agttcagcga gatctttttc
    12301 attcacgcgg cgctggcaca attcatagcc aaacggcggc gcaacggaaa cggtgccgcg
    12361 atttttactg atcaatttaa gccattgcag aggacgcatg gcaaaatcct gagtgcgcaa
    12421 ataatctact gaaagctgcg tggagacggg ggtcaggaga aagccgacca gtcccatatc
    12481 atggtagaaa ggcagccagg agacgcagcg gtcgccaggg cgtaatttaa tgccgtcgtg
    12541 gcttatagca cgtagattag ccatcacttc gcgatgggtg ataatgacgc cacggggaaa
    12601 acgggtgctg ccggaggtgt actggaggta ggcgatatcg ttcggaactg gacgctggag
    12661 cgcaacatcg gcttccggta atgccttaaa ccaggcgtgg cttaaaacat gtaattcggg
    12721 gttgttatgc gtcgcggcat tgaccagtgg caaccactca tcaccagtga taatggctgc
    12781 gggctggcaa cttgccagta aaccctgcaa tttggcgctc caggaatccc gctgaccaac
    12841 gcccattgga atcgccaacg ggacggcgac taagccggca tactggcagg caaaaaaagc
    12901 ctctacgaac ccgctacttg tttcggcaat cagtgccacg cgatcgcctt ttttcaggtt
    12961 cagcgataac aaccgcttcg caccagcttc ggcacgtgct tttaacgtct gatattccag
    13021 ttgatcttca agttggcaac gtctgtcata aaaattcatt ccggcgctac tcagagcggc
    13081 gtagtccaaa gcatcaacca gcgttggaaa atcggcatag cgcataggta gggaatgcgt
    13141 aaagatttta ttagacatat acaccacgaa aaaattggat atccaa
//
LOCUS       DAZGVQ010000137        13118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000137 DAZGVQ010000000
VERSION     DAZGVQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.138"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..68)
                     /gene="yojO"
                     /locus_tag="ACHLAG_003931"
     CDS             complement(<1..68)
                     /gene="yojO"
                     /locus_tag="ACHLAG_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFP0657069.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKR"
     gene            177..671
                     /gene="napF"
                     /locus_tag="ACHLAG_003932"
     CDS             177..671
                     /gene="napF"
                     /locus_tag="ACHLAG_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFP0657070.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYDCAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            661..924
                     /gene="napD"
                     /locus_tag="ACHLAG_003933"
     CDS             661..924
                     /gene="napD"
                     /locus_tag="ACHLAG_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFP0657071.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            921..3407
                     /gene="napA"
                     /locus_tag="ACHLAG_003934"
     CDS             921..3407
                     /gene="napA"
                     /locus_tag="ACHLAG_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFP0657072.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPG
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3414..4109
                     /gene="napG"
                     /locus_tag="ACHLAG_003935"
     CDS             3414..4109
                     /gene="napG"
                     /locus_tag="ACHLAG_003935"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFP0657073.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4096..4959
                     /gene="napH"
                     /locus_tag="ACHLAG_003936"
     CDS             4096..4959
                     /gene="napH"
                     /locus_tag="ACHLAG_003936"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFP0657074.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGSLLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4956..5405
                     /gene="napB"
                     /locus_tag="ACHLAG_003937"
     CDS             4956..5405
                     /gene="napB"
                     /locus_tag="ACHLAG_003937"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFP0657075.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5415..6017
                     /gene="napC"
                     /locus_tag="ACHLAG_003938"
     CDS             5415..6017
                     /gene="napC"
                     /locus_tag="ACHLAG_003938"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFP0657076.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6030..6653
                     /gene="ccmA"
                     /locus_tag="ACHLAG_003939"
     CDS             6030..6653
                     /gene="ccmA"
                     /locus_tag="ACHLAG_003939"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFP0657077.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTGAA"
     gene            6650..7312
                     /gene="ccmB"
                     /locus_tag="ACHLAG_003940"
     CDS             6650..7312
                     /gene="ccmB"
                     /locus_tag="ACHLAG_003940"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFP0657078.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7354..8091
                     /gene="ccmC"
                     /locus_tag="ACHLAG_003941"
     CDS             7354..8091
                     /gene="ccmC"
                     /locus_tag="ACHLAG_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFP0657079.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8088..8297
                     /gene="ccmD"
                     /locus_tag="ACHLAG_003942"
     CDS             8088..8297
                     /gene="ccmD"
                     /locus_tag="ACHLAG_003942"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFP0657080.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8294..8773
                     /gene="ccmE"
                     /locus_tag="ACHLAG_003943"
     CDS             8294..8773
                     /gene="ccmE"
                     /locus_tag="ACHLAG_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFP0657081.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8770..10713
                     /gene="ccmF"
                     /locus_tag="ACHLAG_003944"
     CDS             8770..10713
                     /gene="ccmF"
                     /locus_tag="ACHLAG_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFP0657082.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSIYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGITFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10710..11267
                     /gene="dsbE"
                     /locus_tag="ACHLAG_003945"
     CDS             10710..11267
                     /gene="dsbE"
                     /locus_tag="ACHLAG_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFP0657083.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11264..12316
                     /gene="ccmH"
                     /locus_tag="ACHLAG_003946"
     CDS             11264..12316
                     /gene="ccmH"
                     /locus_tag="ACHLAG_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFP0657084.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12351..12998)
                     /gene="narP"
                     /locus_tag="ACHLAG_003947"
     CDS             complement(12351..12998)
                     /gene="narP"
                     /locus_tag="ACHLAG_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFP0657085.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
BASE COUNT         2738 a         3344 c         3903 g         3133 t
ORIGIN      
        1 cgtttttgcc acgcttcccg ccactctttt gatcctgcta caggttttac cccgatcggg
       61 gtatgcatct ttgacacatc ctttaatatc ttagcggcta taaaaatggc ttattaatta
      121 tgcggtttat ttggtcgctc tcaattttca gagcgcgtta atgatggaag gtcaatgtga
      181 agattgatgc atcccgtcgg ggcatactca ctggtcgctg gcgcaaagcc agtaacggta
      241 tccgtccgcc ctggtccggt gatgaatctc attttctgac ccattgcacc cgctgcgacg
      301 cctgtattaa tgcttgtgaa aacaacattc tgcaacgcgg cgcgggcggc tatccgagcg
      361 ttaatttcaa aaataacgag tgcagcttct gttacgactg cgcgcaggct tgccccgaat
      421 cgctattttc tccgcgccac accagggcct gggatttgca gttcaccatt ggggacgcgt
      481 gcctggcgta tcagtctgtt gaatgccgcc gctgtcagga tagctgtgag ccaatggcaa
      541 ttatctttcg cccgacgctc tccgggatct accagccgca acttaatagc caactttgca
      601 acggatgtgg agcatgtgca gccagctgtc cggtatcagc cataactgcg gagtatctcc
      661 atgcacacta actggcaagt ttgcagcctg gtcgtgcagg ccaaaagcga acgaatttca
      721 gacatcagca cccaactgaa cgcctttccc ggctgtgaag ttgctgtcag cgacgcgccg
      781 agcggtcagt tgattgtggt ggtggaagca gaagacagcg aaacgctgat ccaaaccatt
      841 gagtcagtac gcaacgtaga gggcgtgctg gcggtgtcgc tggtttatca ccagcaggaa
      901 gagcaaggtg aggaaacacc atgaaactca gtcgtcgtag ctttatgaaa gctaacgccg
      961 ttgcggccgc tgcggcggct gccggtctca gcgtgccggg cgttgcccgc gccgttgttg
     1021 gtcagcagga agccattaaa tgggataaag cgccgtgccg tttctgcggt actggttgcg
     1081 gcgttctggt cggaacgcag caggggcgtg tggtggcctg tcagggcgac ccggacgcac
     1141 cggttaaccg tggcctgaac tgcattaagg gctatttcct gcccaagatc atgtacggta
     1201 aagaccgttt gacgcagccg ctgctgcgta tgaaaaacgg taaatatgac aaagaaggcg
     1261 aatttacccc aatcacctgg gatcaggcct tcgatgtgat ggaagagaaa ttcaaaaccg
     1321 ccctgaaaga aaaaggaccg gaatcgatcg gtatgttcgg ttctggtcag tggactatct
     1381 gggaaggtta tgccgcgtcc aagctgttca aagcgggctt ccgttcgaac aacatcgacc
     1441 cgaacgcacg tcactgtatg gcgtcggcag tagttggctt tatgcgtacc tttggtatgg
     1501 atgagccgat gggctgctat gacgacatcg agcaggctga cgcgtttgtg ctgtggggcg
     1561 ctaacatggc ggagatgcac ccgatcctct ggtcacgcat cactaaccgt cgtctctcta
     1621 accagaacgt caccgtggcg gtgctttcta cctaccagca tcgtagcttc gagctggcgg
     1681 ataacggcat catctttacg ccgcaatctg acctggtgat cctgaactac atcgccaact
     1741 atatcattca aaacaatgcg ataaatcagg acttcttcag caagcacgtt aacctgcgca
     1801 aaggggcgac ggacatcggc tacggtttac gtccgaccca tccgctggaa aaagcagcga
     1861 agaatccggg ttctgacgcc tccgaaccga tgagctttga agattacaaa gccttcgttg
     1921 ctgagtatac gctggaaaaa accgctgaaa tgaccggcgt gccgaaagac cagttagaac
     1981 aactggcgca gctgtatgcc gatccgaaca agaaagtcat ctcctactgg acgatgggct
     2041 tcaaccagca tacccgtggt gtgtgggcca acaacctggt ctacaacctg cacctgctga
     2101 ccggcaaaat ttcccagccg ggttgcggtc cgttctccct gaccgggcag ccttctgcgt
     2161 gtggtactgc gcgtgaagtg ggcacctttg ctcaccgtct gcctgcggac atggtggtga
     2221 ctaacgagaa acatcgtgat atctgcgaga agaagtggaa tatcccgggc ggcaccattc
     2281 cggcaaaaat cggtctgcac gcggtggcac aagaccgtgc gctgaaagac ggcaagctga
     2341 atgtttattg gaccatgtgt accaacaaca tgcaggccgg gccgaacatt aatgaagagc
     2401 gtatgccggg ctggcgcgat ccgcgcaact tcatcatcgt ctccgatccg tatccgacag
     2461 tcagtgcgct ggccgccgac ttgatcctgc cgaccgcaat gtgggtagag aaagagggcg
     2521 cttacggtaa cgccgaacgc cgtactcagt tctggcgtca gcaagtacag gcaccgggcg
     2581 aagcgaaatc ggatctctgg cagttagtcc agttctcccg ccgcttcaaa actgaagaag
     2641 tatggccgga agagctgctg gcgaagaaac cggaactgcg tggcaaaacg ctgtacgaag
     2701 tgctgtatgc cacaccggaa gtgagcaaat tcccggtatc cgaactggcg gaagatcagc
     2761 ttaatgatga atcccgcgag ctgggcttct atctgcaaaa agggctgttc gaagagtacg
     2821 catggtttgg tcgcggtcac ggtcacgatc tggcaccgtt cgatgactac cacaaagcgc
     2881 gcggtctgcg ctggccggtg gtgaacggta aagaaacgca gtggcgttac agcgaaggta
     2941 acgacccgta cgtgaaagcg ggcgaaggct ataagttcta cggtaaaccg gatggtaaag
     3001 cggtgatctt cgcgctgccg ttcgaaccgg cggcggaagc accggatgaa gagtacgacc
     3061 tgtggctctc taccggacgc gtcctggagc actggcacac cggcagtatg actcgccgtg
     3121 tgccggaact gcaccgcgcc ttcccggaag cggtcctgtt tattcacccg ctggatgcga
     3181 aagcgcgcga tctgcgccgt ggcgacaaag tgaaagtggt ttctcgccgt ggcgaagtga
     3241 tctcgattgt cgaaacgcgc ggtcgtaacc gtccgccgca gggcctggtg tacatgccgt
     3301 tcttcgacgc cgcacagctg gttaacaaac taacgctgga cgcgaccgat ccgctctcga
     3361 aagagacgga cttcaagaag tgcgcagtca aactggagaa ggtgtaagcc attatgtccc
     3421 ggtcagcgaa acctcaaaat ggtcgccgcc gctttctgcg cgatgttgtt cgcacagcag
     3481 gcgggctggc tgccgtgggt gtggcgctgg ggttacaaca gcaaaccgca cgcgcatctg
     3541 gcgtgcggtt gcgcccgccc ggagccataa acgagaacgc ctttgccagt gcctgtgtgc
     3601 gttgcggtca gtgtgttcag gcttgccctt acgacacctt aaaactggcg acgctggctt
     3661 ctggcctgtc ggcgggcacg ccatattttg tcgcacggga tattccttgc gaaatgtgtg
     3721 aggacattcc gtgcgccaaa gtgtgcccaa gcggtgcgct ggatcgtgag attgaatcga
     3781 tcgacgacgc gcggatgggg ctggcggtac tggtggacca ggaaaactgt ctcaactttc
     3841 aggggctgcg ctgcgatgtt tgttatcgcg aatgcccgaa aattgatgag gccatcaccc
     3901 tggagctgga gcgcaacacg cgtaccggta agcacgcccg ctttctgccg acggttcaca
     3961 gcgacgcctg taccggttgc ggtaagtgcg aaaaagtatg cgtgctggaa caaccggcaa
     4021 tcaaggtgtt accgctgtca ctggcgaaag gggagttagg tcaccattac cgcttcggct
     4081 ggctggaggg gaacaatggc aaatcgtaaa cgtgacgccg ggcgcgaggc gctggagaaa
     4141 aaaggctggt ggcgcagtca ccgttggctg gtgttacgtc gcctttgtca gttcttcgtg
     4201 ctggggatgt ttttgagcgg tccgtggttt ggtgtgtgga tcttacacgg taactacagc
     4261 agtagcctgt tattcgacac cgttccgctg accgatccgc tgatgacgct gcaaagtctt
     4321 gccagtggtc atctgcctgc cacggtggcg ttgaccggtg cggtgattat caccgtgctc
     4381 tacgccctgg caggaaagcg attattttgc agctgggtct gcccgctgaa tccgattacc
     4441 gacttagcga actggttacg caggcgcttt gacctcaatc agtctgcgac gatccctcgc
     4501 cacattcgct acgtgctgtt ggtggtcatt ctgctgggat cggccttaac tggcacgctc
     4561 atctgggaat ggatcaaccc cgtatctctg atggggcgca gcctggtaat gggcttcggc
     4621 agcggctcgc tgcttattct cgcgctgttt ttatttgatc tactggtcgt tgaacacggc
     4681 tggtgcgggc acatttgtcc ggttggcgcg ctgtatggcg tgctgggtag caaaggtgtg
     4741 attaccgttg cggccagcga tcggcagaaa tgtaaccgct gtatggattg ttttcatgtt
     4801 tgcccggaac cgcatgtgct acgtgccccg gtgctggatg aacaaagccc ggtgcaggtc
     4861 accagccgcg attgcatgac gtgcggtcgc tgcgtggatg tctgttctga ggatgtattt
     4921 acaataacta cacgatggag ttcgggagcg aaatcatgaa aagccatgac ctgaagaaag
     4981 cgctgtgtca atggacggcg atgctggccc tggtggtaag cggcgcggtt tgggcggcta
     5041 atggcgtcga ttttagccag tcgccggaag tatccgggac acaggaaggg gccattcgga
     5101 tgccaaaaga gcaggaacgg atgccgctga actatgtgaa tcagccgcca atgatcccgc
     5161 atagcgttga aggttatcag gtaacgacca ataccaaccg ctgcttgcag tgccacggtg
     5221 tcgaaagtta tcgcaccact ggcgcgccgc gtatcagtcc tactcacttt atggacagcg
     5281 acggcaaagt gggcgcggaa gtggctccgc gtcgttattt ctgtctgcaa tgtcacgtac
     5341 cgcaggccga taccgcgcca atcgtgggga atacctttac cccatcaaaa ggttacggga
     5401 aataagaggt tattatggga aattctgacc gtaagcctgg tctgattaag cgcctgtgga
     5461 aatggtggcg tacccccagc cgtctggcgc tggggacgct gctgttgatc ggttttgtcg
     5521 gcggcatcgt cttctggggc ggctttaaca ctgggatgga aaaagccaat accgaagagt
     5581 tctgcattag ctgccacgaa atgcgtaaca cggtgtatca ggaatacatg gattccgtgc
     5641 actacaacaa ccgtagcggc gtccgtgcga cctgtccgga ttgtcacgtt ccgcacgagt
     5701 ttgtgccgaa gatgatacgc aagctcaaag caagtaaaga gctgtatggt aaaatttttg
     5761 gcgttattga cacgccgcag aaatttgaag ctcatcgtct gacgatggca cagaatgagt
     5821 ggcggcgcat gaaggacaat aactcgcagg agtgccgtaa ctgtcacaac ttcgagtata
     5881 tggatacaac cgcccagaaa tcggttgccg cgaagatgca tgaccaggcg gtgaaagatg
     5941 ggcaaacctg tattgattgc cataaaggga tagcgcacaa gctgcccgat atgcgtgaag
     6001 tcgagccagg tttttaacag ggttattgcg tgggtatgct tgaagccaga gagttacttt
     6061 gtgagcggga tgaacgaacc ttatttagtg gcttgtcatt tacgctgaac gcaggagagt
     6121 gggtacaaat caccggtagc aacggcgcgg ggaagacaac gcttctccgt ttgctgactg
     6181 ggttgtctcg ccctgacgct ggcgaggttc tctggcaagg gcagcccttg catcaggtac
     6241 gcgacagcta ccatcaaaac ctgttatgga taggccatca gccggggatc aaaacccggc
     6301 tgacggcgtt agaaaatctg cacttttatc atcgcgatgg cgatgccgca caatgtctgg
     6361 aagccctggc gcaggccggg cttgccggat tcgaagatat tccggtaaat caactttctg
     6421 ccgggcaaca acgccgcgtc gctttagcgc gtctgtggct gacccgtgcc acgttatgga
     6481 tcctcgacga gccttttacc gcgattgacg ttaacggcgt cgatcgtctg acccagcgta
     6541 tggcgcagca tacggagcag ggggggattg tgattctgac tacccaccag ccgctcaacg
     6601 ttgctgaaag taaaattcgc cgcatttcac tgacgcaaac gggggccgca tgatgttctg
     6661 gcgcattttc cgtcttgagc tgcgcgtagc gtttcgccat agcgccgaaa tcgccaaccc
     6721 gctgtggttc ttcctgattg taattaccct ttttccgctc agtatcggtc cggagccgca
     6781 actgctggcg cgtattgcac cgggcattat ctgggttgct gcgctgcttt catccttgct
     6841 ggcgctggaa cgactgttcc gtgacgattt gcaggacggc agtcttgaac aattgatgtt
     6901 gttgccgtta cccttgcccg ccgttgtgct ggcgaaggtg atggcgcact ggatggtaac
     6961 cggtctgccg ttactcatcc tttcgccact ggtagcaatg ctactgggaa tggatgttta
     7021 tggctggcaa gtgatggcgc tgacgctgct gctgggaacg cctacgcttg gctttctcgg
     7081 tgcaccgggt gtggcgttga ctgtgggact taagcgcggt ggtgtgctgc tcagcatact
     7141 ggtgttaccg ctgactatcc cattactcat ctttgccacc gccgcgatgg acgcggcttc
     7201 catgcatttg cccgttgacg ggtatctggc aattttaggc gcgttgctgg caggcaccgc
     7261 gacattaagt ccttttgcga cggcggcagc gttacgaatc agcattcaat aacgctggat
     7321 tactgatttt tgttcgagtc tggtatcgaa actatgtgga aaacactgca tcaactggcg
     7381 atcccaccac ggctgtatca aatctgtggc tggtttatac cgtggctggc aattgccagt
     7441 gtggtcgtgc ttaccgtcgg ctggatctgg ggattcggct ttgctccggc tgattatcag
     7501 cagggaaata gctaccgcat tatctacctg catgtgcctg cggcgatctg gtcgatgggc
     7561 atttatgcat caatggcggt ggcggctttt attggccttg tctggcagat gaaaatggcc
     7621 aacctggcgg tggcggcgat ggcccccatt ggtgccgtgt ttacctttat tgccctggtt
     7681 accggctctg catggggaaa accgatgtgg ggcacctggt gggtatggga tgcacgtctg
     7741 acttctgaac tggtgctgct gtttttgtat gtgggtgtga ttgccctgtg gcacgccttc
     7801 gacgaccgcc gtctggcggg ccgtgcggca ggtatcctgg tgctgattgg cgtggtgaat
     7861 ctgccgatta ttcattactc cgtggagtgg tggaacaccc tgcatcaggg atcaacgcgg
     7921 atgcagcaaa gtatcgatcc ggcgatgcgt tcgccgctgc gctggtcgat ttttggcttc
     7981 ctgctgctgt ctgccacgct gacgctgatg cggatgcgta atttgatttt gctgatggaa
     8041 aaacgccgtc cgtgggtgag tgaactgata ctgaaaagag gccgtaaatg acccctgcat
     8101 ttgcttcctg gaatgaattt ttcgcaatgg gcggttacgc cttttttgtc tggctggcgg
     8161 tggtgatgac cgttattccg ctggtggttt tggtcgtgca ctcggtgatg cagcatcgcg
     8221 caattctgcg tggtgtggcg caacagcggg cgcgtgaggc gcgtttacgt gctgcgcaac
     8281 agcaggaggc tgcatgaata ttcgccgtaa aaaccgcttg tggattgcct gtgccgtgtt
     8341 ggcagggctg gcgctgacta tcggtctggt gctatatgcg ctgcgctcga atatcgatct
     8401 cttttatacg ccgggggaaa ttctctacgg caagcgtgaa actcagcaaa tgccggaagt
     8461 cgggcagcgt ctgcgcgtcg gcgggatggt gatgccgggt agcgtgcagc gcgatcccaa
     8521 ttcgctaaaa gtaactttca ccatttacga cgctgaaggt tcagtagatg tctcttacga
     8581 aggcattttg ccggatctgt tccgtgaagg gcagggcgtt gtggtgcagg gcgagctgga
     8641 aaaaggcaat catatcctcg cgaaagaagt gctggcgaaa cacgacgaaa actacacgcc
     8701 gccagaagtt gagaaagcga tggaagctaa ccaccgtcgc ccggcgagtg tttataagga
     8761 cccagcatca tgatgccgga aattggaaac gggctgctgt gcctggcgtt agggattgcg
     8821 ctgctgctgt ccgtgtatcc gctatggggc gtggcgcgcg gagatgcgcg catgatggcg
     8881 tcttcccgct tgtttgcctg gctgctgttt atgtctgtgg ctggcgcatt tctggtgctg
     8941 gtcaatgctt tcgtggtcaa cgactttacc gtcacctatg ttgccagcaa ctccaatacc
     9001 cagcttccgg tgtggtatcg cgtggcggct acctggggcg cgcatgaagg ctcgctactg
     9061 ctgtgggtgc tgctgatgag cggctggacc tttgcggtgg cgatttttag tcagcgtatt
     9121 ccgctggata ttgtggcccg cgtactggcg ataatgggga tggtcagcgt cggctttttg
     9181 ctgttcattc tctttacctc taacccattc tcacgcacgc tgccgaactt cccgattgaa
     9241 gggcgcgatc ttaacccgct gttacaggat ccggggctga tcttccatcc gcctctgctt
     9301 tatatggggt acgtgggttt ctcggtggcg tttgcttttg ccattgcttc tttgttgtgc
     9361 gggcgtctgg acagcattta tgcgcgtttt actcgtccgt ggacgctggc ggcgtggatc
     9421 ttcttgacgc tcggcattgt gctcggttcc gcatgggcct attacgaact cggctggggc
     9481 ggctggtggt tctgggatcc ggtagaaaac gcctcgttta tgccgtggct ggtggggact
     9541 gcgctgatgc actcactggc ggtcactgaa caacgcgcca gcttcaaagc gtggacatta
     9601 ctgctggcaa tcagtgcctt ctcgttgtgt ctgctgggga ctttcctggt gcgttccggc
     9661 gtgctggtat cggtacacgc gtttgcctct gatccggcac gcggtatgtt tatcctcgcc
     9721 tttatggtac tggtgattgg tggttcgctg ctgctgtttg ccgcgcgtgg acacaaagtt
     9781 cgctcacgcg taaacaatgc gctgtggtcg cgggaatctc tgctgttagc gaacaacgtt
     9841 ttgctggtcg ccgcgatgct ggtggtatta ctggggacgc tgctgccgct ggtgcacaag
     9901 caactgggac tgggcagtat ttcgattggc gaaccgttct tcaacaccat gtttacctgg
     9961 ctgatggtgc cgtttgcgct gctgcttggt gtcggtcctc tggtgcgctg ggggcgcgat
    10021 cgcccacgta aaatccgcaa tttattgatt atcgccttca tctcaacgct ggtgctgtcg
    10081 ctgctgctgc cgtggctgtt cgaaagcaaa gttgtggcga tgacggtgct cggcctggca
    10141 atggcctgct ggattgctgt gctggcaatt gcggaagctg cgctgcgtat ttcacgtggc
    10201 acgaaaacca ccttcagtta ttggggaatg gtggcggctc acctggggct ggcagtgaca
    10261 attgttggta ttacctttag ccagaactat agcgttgagc gtgatgtgcg catgaagtcc
    10321 ggcgatagcg tcgatattca tgaatatcgc ttcaccttcc gtgatgtcaa agaggtgact
    10381 ggcccgaact ggcgtggcgg tgtggcgact atcggcgtaa cgcgcgatgg caagccggaa
    10441 acggtgctgt atgcggaaaa acgttattac aacactgccg ggtcgatgat gaccgaagcg
    10501 gcaattgacg gcggcatcac gcgtgacctg tacgccgcgc tcggtgaaga gctggaaaac
    10561 ggcgcgtggg ccgtgcgtct ttactacaaa ccatttgttc gctggatttg ggcgggcggg
    10621 ctgatgatgg cgttgggcgg actgctgtgt ctgtttgatc ctcgctatcg taagcgcgtg
    10681 agtccgcaaa aaactgcgcc ggaggccgta tgaagcgcaa agtattgtta attccgttga
    10741 ttatcttcct ggcgattgcc gcggcgctgc tgtggcagct ggcgcgtaat gccgaagggg
    10801 atgatccgac caatctggaa tcggcgctca ttggcaagcc tgtgccgaag tttcgtctcg
    10861 aatcactgga caatccgggg cagttttatc aggcggatgt gctgactcag ggcaaaccag
    10921 tgctgcttaa cgtctgggcg acctggtgtc cgacctgccg tgcggaacat caatatctga
    10981 atcagctttc agcgcagggc attcgcgtgg tcggcatgaa ctataaagac gatcgccaga
    11041 aggcaatcag ctggctgaaa gagctgggca atccttacgc gctaagcctg tttgatggcg
    11101 acggcatgtt agggctggat ctcggtgtct atggcgcgcc agaaaccttc cttattgacg
    11161 gcaacggtat cattcgctat cgccatgcgg gcgatctcaa tcctcgcgtc tgggaagaag
    11221 agatcaagcc gctgtgggag aaatacagta aggaggccgc acaatgaggt ttttattggg
    11281 cgtgctgatg ctgatgatct ccggctcagc actggcgacc atcgacgtgt tgcagtttaa
    11341 agatgaagca caggaacaac agttccgtca gctcactgaa gaactgcgct gcccgaaatg
    11401 ccagaacaac agcattgccg attccaactc gatgattgcc accgacctgc gtcagaaagt
    11461 gtatgaactg atgcaggaag gtaaaagtaa aaaagagatt gtcgattata tggtggcgcg
    11521 ttacggcaac ttcgtcactt acgatccgcc gttaacgccg ctgaccgtgc tgctgtgggt
    11581 gctgccggta gtggctattg gcattggcgg ttgggtcata tacgcccgct cgcggcgtcg
    11641 ggtacgcgta gtgccggaag cgtttcctga acaaagcgtg caggaaggta agcgtgccgg
    11701 atatattgtt tatctgccgg gtattgtggt ggcgttaatt gtggctggcg tcagctacta
    11761 ccagactggc aattatcagc aggtgaaaat ctggcagcag gccacggcac aggctccggc
    11821 gttgctggac agggcgctgg atccgaaagc cgatccgctc aacgaagaag agatgtcgcg
    11881 tcttgcgctg gggatgcgta ctcaactgca aaaaaatccg ggagatatag aaggctggat
    11941 tatgttgggc cgcgttggca tggcgctggg taacgctagt atcgccaccg atgcatacgc
    12001 tactgcgtat cgcctcgatc cgaaaaacag cgatgccgca ctgggttatg ctgaagcgtt
    12061 gacacgttca tctgatccca acgacaaccg cctcggtggt gaactgctgc gccagttggt
    12121 gagaagcgac cacagcaata tccgtgtgtt aagcatgtat gcgtttaatg cctttgagca
    12181 gcagcgattt ggcgaagccg ttgccgcgtg ggagatgatg ttgaaactct tacctgccaa
    12241 cgatactcgc cgtgcggtga ttgaacgtag tatcgcgcag gcgatgcaac atttgtcgcc
    12301 gcaggagagt aaataaaaag aggcccgatg gtgatgccat cgggctattt ttattgtgcc
    12361 ccgcgttgtt gcaggaacag aatggtggct gccacgcgtg agcggacatt gagtttgcgc
    12421 agcagattgc gaatatgtac ttttactgtc tgctctgaaa tattcaacac cgaggcaatc
    12481 tgtttatttg acagcccctg tgccagctcg tgcagaacat ccagctcgcg ctccgtcagc
    12541 acgctgaagg gatcttcttc cgcgccaaac atttcacgtt cacgtaagta ctgattgacg
    12601 cgttcgctaa agactttgct gcctttcgct ccagcacgaa ttgcttccag caatacttcc
    12661 gggtcgctgt ctttcaacag ataaccgtct gcgcctgcgt ctatcagtgc aaagacatcg
    12721 ctggaggcat ctgatacggt caggataata atttgcgcgg taacgccatc cctgcgcaag
    12781 gcattgaggg tatccaggcc actcatacct ttcatattga gatccagcaa gatcacgtcg
    12841 atatccagtc tattcgccag atcgatcgcg ctcgcgccgt cgcccgcttc ggcgaccact
    12901 tcaaagccag gatcaagctc cagtaactga cgaacaccgc gtcgcataag tggatgatca
    12961 tccacaatca tcacctgaaa aggcgttgct tcaggcatag tagtctcctg aggttttatt
    13021 agaatcatta tgtttgagcg tagcgttggt aacgctacca ggcgcgtgaa tgttacccca
    13081 ttttatgtat ggacatgtag tgaaaactac aaccttgt
//
LOCUS       DAZGVQ010000138        13048 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000138 DAZGVQ010000000
VERSION     DAZGVQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.139"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            228..1337
                     /gene="livK"
                     /locus_tag="ACHLAG_003948"
     CDS             228..1337
                     /gene="livK"
                     /locus_tag="ACHLAG_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFP0657086.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1385..2311
                     /gene="livH"
                     /locus_tag="ACHLAG_003949"
     CDS             1385..2311
                     /gene="livH"
                     /locus_tag="ACHLAG_003949"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFP0657087.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTVFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2308..3585
                     /gene="livM"
                     /locus_tag="ACHLAG_003950"
     CDS             2308..3585
                     /gene="livM"
                     /locus_tag="ACHLAG_003950"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFP0657088.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3582..4349
                     /gene="livG"
                     /locus_tag="ACHLAG_003951"
     CDS             3582..4349
                     /gene="livG"
                     /locus_tag="ACHLAG_003951"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFP0657089.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4351..5067
                     /gene="livF"
                     /locus_tag="ACHLAG_003952"
     CDS             4351..5067
                     /gene="livF"
                     /locus_tag="ACHLAG_003952"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFP0657090.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGGQ"
     gene            5460..6776
                     /gene="ugpB"
                     /locus_tag="ACHLAG_003953"
     CDS             5460..6776
                     /gene="ugpB"
                     /locus_tag="ACHLAG_003953"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFP0657091.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6874..7761
                     /gene="ugpA"
                     /locus_tag="ACHLAG_003954"
     CDS             6874..7761
                     /gene="ugpA"
                     /locus_tag="ACHLAG_003954"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFP0657092.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7758..8603
                     /gene="ugpE"
                     /locus_tag="ACHLAG_003955"
     CDS             7758..8603
                     /gene="ugpE"
                     /locus_tag="ACHLAG_003955"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFP0657093.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8605..9675
                     /gene="ugpC"
                     /locus_tag="ACHLAG_003956"
     CDS             8605..9675
                     /gene="ugpC"
                     /locus_tag="ACHLAG_003956"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFP0657094.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9672..10415
                     /gene="ugpQ"
                     /locus_tag="ACHLAG_003957"
     CDS             9672..10415
                     /gene="ugpQ"
                     /locus_tag="ACHLAG_003957"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP0657095.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGESLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10402..10842)
                     /gene="yhhA"
                     /locus_tag="ACHLAG_003958"
     CDS             complement(10402..10842)
                     /gene="yhhA"
                     /locus_tag="ACHLAG_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFP0657096.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10962..12704
                     /gene="ggt"
                     /locus_tag="ACHLAG_003959"
     CDS             10962..12704
                     /gene="ggt"
                     /locus_tag="ACHLAG_003959"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFP0657097.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12742..13026)
                     /gene="yrhB"
                     /locus_tag="ACHLAG_003960"
     CDS             complement(12742..13026)
                     /gene="yrhB"
                     /locus_tag="ACHLAG_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFP0657098.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2888 a         3337 c         3747 g         3076 t
ORIGIN      
        1 ttttatgctg acgacggcgc ttttttctgt ttatctatca ataaattcag aatattatct
       61 gttcttaatc gactgaaaaa tagggatttt aatcgctatt atcacaaaat actgcgctaa
      121 acccttaatc agacaggcaa aaacagtgca gtataaaaaa agaacagtct gatttgttaa
      181 cacataaaaa caaagcaaca caacatcacg aatggggatt tttgactatg aaacggaatg
      241 cgaaaactat catcgcaggg atgattgcac tggcaatttc acacaccgct atggctgacg
      301 atattaaagt tgccgttgtc ggcgcgatgt ccggcccgat tgcccagtgg ggcgatatgg
      361 aatttaacgg cgcgcgtcag gcgattaaag acattaatgc caaaggggga attaaaggcg
      421 ataagctggt gggcgtggaa tatgacgacg cctgcgaccc gaaacaagcc gttgcggtcg
      481 ccaacaaaat cgttaacgac ggcattaaat acgttattgg tcatctgtgt tcttcttcta
      541 cccaacctgc gtcagatatc tacgaagacg aaggtattct gatgatctcg ccgggagcga
      601 ccaacccgga gctgacccaa cgcggttatc aacacattat gcgtactgcc gggctggact
      661 cttcccaggg gcctacggcg gcaaaataca ttcttgagac ggtgaagccc cagcgcatcg
      721 ccatcattca cgacaaacaa cagtatggcg aagggctggc gcgttcggtg caggacgggc
      781 tgaaagcggc taacgccaac gtcgtcttct tcgacggtat taccgccggg gagaaagatt
      841 tctccgcgct gatcgcccgc ctgaaaaaag aaaacatcga cttcgtttac tacggcggtt
      901 actacccgga aatggggcag atgctgcgcc aggcccgttc cgttggcctg aaaactcagt
      961 ttatggggcc ggaaggtgtg ggtaacgcat cattgtcgaa tattgctggc gatgctgccg
     1021 aaggcatgtt ggtcactatg ccaaaacgct atgaccagga tccggcaaac cagggcatcg
     1081 ttgatgcgct gaaagcagac aagaaagatc cgtccgggcc ttatgtctgg atcacctacg
     1141 cggcggtgca atctctggcg actgcccttg agcgtactgg cagcgatgag ccgctggcgc
     1201 tggtgaaaga tttaaaagct aacggtgcaa acaccgtgat tgggccgctg aactgggatg
     1261 aaaaaggcga tcttaaggga tttgattttg gtgtcttcca gtggcacgcc gatggttcat
     1321 ccacggcagc caagtgaaaa tcccaccgcc cgtgaaaaac gggcgggttt agaaaggtta
     1381 ccttatgtct gagcagtttt tatatttctt gcagcagatg tttaacggcg tcacgctggg
     1441 cagtacctac gcgctgattg ccatcggcta caccatggtt tacggcatta tcggcatgat
     1501 caacttcgcc cacggcgagg tttatatgat cggcagctat gtctcattta tgatcatcgc
     1561 cgcgctgatg atgatgggca ttgataccgg ctggctgctg gtggcggcgg gattcgtcgg
     1621 cgcaatcgtc atcgccagcg cctacggctg gagtatcgaa cgggtagcct atcgcccggt
     1681 gcgtaactct aagcgcctga ttgcgctcat ctcggcaatc ggcatgtcca tcttcctgca
     1741 aaactacgtt agcctgactg aaggttcgcg tgacgtggcg ctgcccagtc tgtttaacgg
     1801 tcagtgggtg gtggggcata gcgaaaactt ctctgcctct attaccacca tgcaggcggt
     1861 gatctggatt gtcaccttcc ttgccatgct ggcgctgacg gttttcattc gctattcccg
     1921 tatggggcgc gcgtgtcgtg cctgcgcgga agacctgaaa atggcgagcc tgcttggcat
     1981 taacaccgac cgggtgattg cgctgacctt tgtgattggc gcggcgatgg cggcggtggc
     2041 aggtgtgctg ctcggtcagt tctacggcgt aattaacccc tacatcggct ttatggccgg
     2101 gatgaaagcc tttaccgctg cggtgctcgg tgggattggc agcattccgg gagcgatgat
     2161 tggcgggctg attctgggga ttgcggaggc gctctcttct gcctatctga gtacggaata
     2221 taaagatgtg gtctcattcg ccctgctgat tctggtgctg ctggtgatgc cgaccggtat
     2281 tctgggtcgc ccggaggtag agaaagtatg aaaccgatgc atattgcaat ggcgctgctc
     2341 tctgccgcga tgttttttgt cctggcgggc gtctttatgg gcgtgcaact ggagctggat
     2401 ggcaccaaac tggtggtcga cacggcttca gacatccgtt ggcagtgggt gtttatcggc
     2461 acggcggtgg tctttttctt ccagcttttg cgaccggctt tccagaaagg gctaaaaagc
     2521 gtttccggac cgaagtttat tctgcccgcg attgatggct ccacggtgaa gcagaaacta
     2581 ttcctcgtgg cgctgctggt gcttgcggtg gcgtggccgt ttatggtgtc gcgtgggacg
     2641 gtggatatcg ccaccctgac catgatctac attatcctcg gcctcgggct gaacgtggtt
     2701 gttggtcttt ctggtctgct ggtgctgggg tacggcggct tttacgccat cggcgcttac
     2761 acttttgcgc tgctcaatca ctattacggc ctgggcttct ggacctgtct gccgattgcc
     2821 gggttaatgg cagcggcggc cggcttcctg ctcggcttcc cggtgcttcg cttgcgcggc
     2881 gactatctgg cgatcgttac cctcggtttc ggcgaaattg tgcgcatatt gctgctcaat
     2941 aacaccgaaa ttaccggcgg cccgaacgga atcagtcaga tcccgaaacc gacactcttc
     3001 ggactcgagt tcagccgtac cgctcgtgaa ggcggctggg acacgttcag taatttcttt
     3061 ggcctgaaat acgatccctc cgatcgtgtc atcttcctct acctggtggc gttgctgctg
     3121 gtggtgctaa gcctgtttgt cattaaccgc ctgctgcgga tgccgctggg gcgtgcgtgg
     3181 gaagcgttgc gtgaagacga gatcgcctgc cgttcgctgg gcttaagccc gcgtcgtatc
     3241 aagctgactg cctttaccat aagtgccgcg tttgccggtt ttgccggaac gctgtttgct
     3301 gcgcgccagg gctttgtcag cccggaatcc ttcacctttg ctgaatcggc gtttgtactg
     3361 gcgatagtgg tgctcggcgg tatgggatcg cagtttgcgg tgattctggc ggcaattttg
     3421 ctggtggtat cgcgcgaatt gatgcgtgat ttcaacgaat acagcatgtt aatgctcggt
     3481 ggtttgatgg tgctgatgat gatctggcgt ccgcagggct tgctgcccat gacgcgcccg
     3541 caactgaagc tgaaaaacgg cgcagcgaaa ggagagcagg catgagtcag ccattattat
     3601 ctgttaacgg tctgatgatg cgcttcggcg gcttgctggc ggtgaacaac gtcaatcttg
     3661 aactgtaccc gcaggagatc gtctcgttaa tcggccctaa cggtgccgga aaaaccacgg
     3721 tttttaactg cctgaccgga ttctacaaac ccaccggcgg cactatttta ctgcgtgatc
     3781 agcatctgga aggtttaccg gggcagcaaa ttgcccgcat gggcgtggtg cgtaccttcc
     3841 agcatgtgcg tctgttccgt gaaatgacgg tgattgaaaa cctgctggtg gcgcagcatc
     3901 aacaactgaa aaccgggctg ttctctggcc tgttgaaaac gccttctttc cgtcgcgccc
     3961 agagcgaagc gctcgaccgc gccgccacct ggcttgagcg cattggtttg ctggaacacg
     4021 ccaaccgtca ggcgagtaac ctggcctatg gtgaccaacg ccgtctggag attgcccgct
     4081 gcatggtgac gcagccggag attttaatgc tcgacgaacc ggcggcaggt cttaacccaa
     4141 aagaaaccaa agagctggat gagctgattg ccgaactgcg caatcatcac aacaccacta
     4201 tcttgttgat tgaacacgat atgaagctgg tgatggggat ttcagaccga atttatgtgg
     4261 tcaatcaggg gacgccactg gcaaacggta cgccggagca aatccgtaat aacccggacg
     4321 tgatccgtgc ctatttaggt gaggcataag atggaaaaag tcatgttgtc ctttgacaaa
     4381 gtcagcgccc actacggcaa aatccaggcg ctgcatgagg tgagcttgca tatcaatcag
     4441 ggcgagattg tcacgctgat tggcgcgaac ggggcgggga aaaccacctt gcttggtacg
     4501 ttatgcggcg atccgcgcgc caccagcggg cgaattgtgt ttgatgataa agacattacc
     4561 gactggcaga cggcgaaaat catgcgcgaa gcggtagcga ttgtcccgga agggcgtcgc
     4621 gtcttctcgc ggatgacggt ggaagagaac ctggcgatgg gcggcttctt tgccgaacgt
     4681 gaccagtttc aggagcgcat aaagtgggtg tatgagctgt ttccacgtct tcatgagcgt
     4741 cgtgttcagc gggcgggcac catgtccggc ggtgaacagc agatgctggc gattggtcgc
     4801 gcgctgatga gcaacccgcg tttgttgttg cttgatgagc catcgctcgg tcttgcgccg
     4861 attatcatcc agcaaatttt cgacaccatc gagcagctgc gcgagcaggg gatgactatc
     4921 ttcctcgtcg agcagaacgc caaccaggcg ctaaagctgg cggatcgcgg ctacgtgctg
     4981 gaaaacggcc atgtggtgct ctccgatacc ggcgacgcgc tgctggcgaa cgaagcagtg
     5041 agaagtgcgt atttaggcgg gcaataacac gttgattgat aaggagtcaa aagactcctt
     5101 tgagacaggt gacaaatgta aaattgcctg atgcgctgcg cttatcaggc ctactgggtg
     5161 agttgcaata tattgaattt gcacgatctt gtaggccgga taaggcgttt acgccgcatc
     5221 cggcatgaaa cgatgagtaa tctgtagagt ttgattcaga ccttctattt tccgcttatc
     5281 cgtgccccat ctcccatttt ccctcaccca cgccgtcacc gccttgtcat ctttctgaca
     5341 ccttactatc ttacaaatgt aacaaaaagg ttatttttct gtaattcgag catgtcatgt
     5401 taccccgcga gcataaaacg cgtgaattcg cgcattcggt acaacaagag agataaacga
     5461 tgaaaccgtt acattataca gcttcagcac tggcgctcgg actggcgtta atggggaatg
     5521 cacaggcagt gacgaccatt ccgttctggc attctatgga aggggaactg ggtaaagagg
     5581 tggattctct ggcccaacgt tttaacgccg aaaatccgga ttacaaaatt gtaccgacct
     5641 ataaaggcaa ctacgaacag aatttaagcg cggggattgc cgcatttcgt accggcaacg
     5701 ctccggctat tttgcaggtt tatgaagttg gcaccgccac catgatggca tcgaaagcca
     5761 ttaaaccggt atatgacgtg tttaaagagg cggggattca attcgatgag tcgcagtttg
     5821 tgccgacggt ttccggttac tactccgaca gcaaaacggg ccacttactc tcccagccat
     5881 tcaacagctc gacccccgtt ctctattaca acaaagacgc cttcaagaaa gcaggattag
     5941 acccggaaca accgccgaaa acctggcagg atctggcgga ctatgccgcg aaactgaaag
     6001 cctccggtat gaagtgcggc tacgccagcg gctggcaggg ctggatccaa ctggaaaact
     6061 ttagcgcctg gaacggtctg ccgtttgcca gcaaaaacaa cggctttgac ggcacggacg
     6121 cggtgctgga gttcaacaag ccggagcagg tgaaacacat cgccatgctc gaggagatga
     6181 acaagaaggg cgacttcagc tacgtcggtc gtaaggatga atccaccgag aagttctata
     6241 acggtgattg cgcgatgacc accgcctctt ccggttctct cgctaatatt cgtgagtacg
     6301 ccaaatttaa ctatggcgta ggcatgatgc cttacgacgc cgatgcgaaa gatgcaccgc
     6361 aaaacgccat tatcggcggg gccagcctgt gggtgatgca gggcaaaaat aaagaaacgt
     6421 ataccggcgt ggcgaagttc ctcgacttcc tcgcgaagcc agaaaacgct gccgagtggc
     6481 atcagaaaac cggttatctg ccaatcacca aagcagcgta tgacctgacc cgtgagcagg
     6541 gcttttatga gaaaaaccca ggggcggata ccgcgacgcg tcagatgctg aataagccgc
     6601 cgttgccgtt caccaaaggg ctgcgtctgg gcaacatgcc gcagatccgc gtgattgtgg
     6661 atgaagagct ggagagcgtg tggaccggta agaagacacc acagcaggca ctggataccg
     6721 ccgttgagcg tggaaatcag ttgctgcgcc gctttgagaa atcgacgaag tcttaatcag
     6781 tgtaatgtcg gatgcgtttc gcttatctga cctggcatcg cgtgtaggcc ggataagcga
     6841 agcgcatccg gcacagttca ggaattaacc gtaatgtcat catcccgtcc ggtgttccgc
     6901 tcgcgctggc tgccttatct gctggtcgcg ccgcagctca tcatcaccgt tatctttttt
     6961 atctggcctg cgggcgaagc gttgtggtac tcgctacaaa gcgtcgatcc gtttggtttc
     7021 tccagccagt ttgtcggcct ggataacttc gtcgcgctgt ttcatgacag ctactatctc
     7081 gactccttct ggacgacgat aaaattcagc acctttgtca ccgtcagcgg tttgctggtg
     7141 tcgctgttct tcgcggcgct ggtggagtac atcgtgcgcg gcagccgttt ctatcaaacc
     7201 ttaatgttgc tgccttacgc cgtggctccc gccgttgccg ccgtattgtg gatcttcctg
     7261 tttaaccccg gtcgcgggct gatcacccat tttctcgcgg agttcggcta cgactggaac
     7321 cacgcgcaaa acagcggtca ggcaatgttt ctggtggtgt ttgcctcagt atggaagcaa
     7381 atcagctaca acttcctgtt cttctatgcc gcgctgcaat ccattccccg ttcgttgatc
     7441 gaagccgcag ccatcgacgg tgcagggccg attcgccgct tctttaagat tgcgctgccg
     7501 cttatcgccc cggtgagttt cttcctgctg gtagtgaacc tggtgtatgc cttcttcgac
     7561 accttcccgg tgatcgacgc cgccacgtcc ggcgggccgg ttcaggccac cacgacgctg
     7621 atttataaga tctaccgcga aggttttacc ggactggatc tggcttcgtc tgccgcacag
     7681 tcggtggtgt tgatgttcct cgtcatcgtg ctgacggtgg tgcagttccg ctatgttgaa
     7741 agcaaggtgc gttaccaatg attgagaacc gtccgtggct gacgatattc agccatacca
     7801 tgctgatcct cgggatcgcg gtgatcctct tcccgctgta cgtggcgttt gtcgcggcga
     7861 cgctggataa acaggccgtc tatgccgcgc cgatgacgct catccccggc acacatctgc
     7921 tggaaaacat ccacaacatc tgggtgaacg gggtaggcac gaatagcgcg ccgttctggc
     7981 ggatgttgct taacagcttt gtgatggcgt tcagcattac gctcggcaaa attaccgtct
     8041 cgatgctctc ggcatttgcc attgtctggt ttcgttttcc gctacgtaac ctcttcttct
     8101 ggatgatttt tatcaccctg atgctgccgg ttgaagtacg tatcttcccg acggtggaag
     8161 tcatcgccaa cctgcagatg ctcgacagct acgccggttt aacgctgccg ctgatggcct
     8221 cggcgaccgc tactttcctg ttccgccagt tctttatgac gctgccggat gagctggtgg
     8281 aagccgcgcg gatcgacggc gcatcgccaa tgcgcttctt ttgcgacatc gtttttccgc
     8341 tctccaaaac taatctggcg gcgctgtttg tgatcacctt tatctacggc tggaatcagt
     8401 atttgtggcc gttgttgatt attaccgacg ttgatctcgg caccaccgtg gcagggatca
     8461 aagggatgat cgccactggc gaaggcacca cggaatggaa ctcggtaatg gcggcgatgt
     8521 tgttaacgct tatccctccg gtggtgattg ttttagtgat gcagcgtgcc ttcgtgcgcg
     8581 gcctggtcga tagtgagaaa taagatggca ggactgaaat tacaggcagt aaccaaaagc
     8641 tgggatggca aaacccaggt gattaaacca ctgacccttg atgtggcgga tggcgaattt
     8701 atcgtgatgg tcgggccgtc tggctgcgga aaatcgacgc tgctgcgcat ggttgccggg
     8761 ctggagcggg tgacggaagg cgatatctgg atcaacgacc agcgcgtgac cgaaatggag
     8821 ccgaaagatc gcgggattgc gatggtgttc cagaactacg cgctttatcc gcatatgagc
     8881 gtcgaagaaa acatggcgtg ggggctgaaa attcgcggca tgggcaagca gcaaatagcc
     8941 gagcgcgtta aagaagcggc gcgcattctg gagctggatg gcctgcttaa gcgccgcccg
     9001 cgcgagctat ccggcggtca gcgtcagcgt gtggcgatgg gtcgcgcgat tgtgcgcgat
     9061 ccggcggtgt tcctgtttga tgagccgctc tctaacctcg atgccaagct gcgcgtgcag
     9121 atgcgccttg agctgcaaca gctgcaccgt cgcctgaaaa cgacttcact ctacgttact
     9181 cacgatcagg ttgaagcgat gacgctcgcc cagcgagtaa tggtgatgaa cggcggcgtt
     9241 gccgaacaga ttggcacacc agttgaagtc tacgaaaagc ccgccagcct gtttgtagcg
     9301 agttttatcg gcagtccggc gatgaacctg ctgacaggcc gcgtgaataa cgaaggcacg
     9361 catttcgaac tggacggcgg tattgagctg ccgctaaacg gtggctaccg tcagtatgcc
     9421 gggcgtaaaa tgactctcgg cattcgcccg gaacatattg cgctaagctc gcaggcagaa
     9481 ggcggcgtac cgctggtgat ggacacgctg gagatcctcg gcgcagataa cctggcgcac
     9541 ggacgctggg gcgagcagaa gctggtggtg cgactggcgc atcaggagcg cccgacggca
     9601 ggcagcacgc tgtggctgca tctggcggaa aatcagctgc atctttttga tggtgaaaca
     9661 ggacaacgag tatgagtaac tggccttatc cccgcatcgt cgctcatcgt ggcggcggta
     9721 agctggcccc ggaaaacacc ctggcggcaa tcgacgtcgg ggcaaaatac ggtcataaga
     9781 tgatcgaatt tgacgcgaag ttatcgaaag atggcgagat cttcctgctc catgacgaca
     9841 atctcgaacg caccagcaac ggctggggcg tcgcgggtga actgaactgg caggatttac
     9901 tgcgcgtgga tgcgggcagt tggtacagca aagcgtttaa aggtgagtcg ctgccgttgc
     9961 tttcgcaggt ggcggaacgc tgtcgcgaac acgggatgat ggcgaatatc gaaatcaaac
    10021 ccaccactgg cactggacca ttaacgggca aaatggtggc gctggcggca cgccaactgt
    10081 gggccggtat gacgccgccg ctgctgtcat cgtttgagat tgatgcttta gaagctgcac
    10141 agctggcggc accggaactg ccgcgtggtt tattgcttga tgagtggcgc gacgactggc
    10201 gcgaactgac cgcgcggctg ggctgcgtct ctattcatct caatcataag ttactcgata
    10261 aagtgcgagt gatgcagttg aaagacgccg gactgcggat tctggtttat accgtcaata
    10321 aaccccagcg cgcggcagag ttgctgcgct ggggcgtgga ttgcatctgt accgatgcga
    10381 ttgacgtgat tggtccgaac tttacggccc aatagttttc aacggaatgt caggctgcgg
    10441 cgtgctgggc tgattcagca tgtcgccgtt tctcctctcc ggcagcatat gctgctgatt
    10501 caacgaacta tccggattac ggttgctgtt taacatcccg ccgttggtgt tgggcagcat
    10561 ttgctgccgg gcgggatttc gctcccccgg ctgtgactgc aacacccgct gagaattatt
    10621 gtttatctgg ttttctaaat gctgctgttg caactgagtt tgcgttttca gttgctgatt
    10681 cagcatccct ttttgctgga tttgctgagt ctgcatctgg gtttgcatcc gctgctggct
    10741 gggtatctga taccccggct ggttagggtt gttcagagta ttaatgggct gtgcaaagcc
    10801 gacaaacggc aggagtgccg taagaatcag aagtcgtttc atcgcgtatc ctcctctgaa
    10861 gatatccttt aagtttactc gcttcccgac aaaacgatga ttaattcaga gttatatacc
    10921 aggcttagct ggggttgccc cttaatctct ggagaataac gatgataaaa ccgacgtttt
    10981 tacgccgggt ggccattgct gctctgctct caggaagttg ttttagcgcc gccgccgcgc
    11041 ctcctgcacc gcccgtctcg tatggtgtgg aggaagatgt cttccacccg gtacgcgcga
    11101 aacagggaat ggtagcgtct gtggacgcca ctgccactca ggtgggggtg gatattctca
    11161 aggagggcgg gaatgccgtt gatgccgccg tggcggtggg ctacgcgctg gcggtaacgc
    11221 atccgcaggc agggaatctg ggcggtggtg gttttatgct gatccgctcg aaaaatggca
    11281 ataccaccgc tatcgatttc cgcgaaatgg cacccgctaa agcgacccgc gatatgttcc
    11341 ttgatgacca gggcaacccg gacagcaaaa aatcactcac ttcgcatctg gcttccggca
    11401 caccaggtac ggtcgctggt ttctcgctgg cgttggataa gtacggcact atgccgttga
    11461 acaaagtggt gcagcccgcg ttcaaactgg cacgcgatgg ttttatcgtt aacgatgctt
    11521 tggcagacga tctcaaaacc tacggtagcg aagtgttgcc gaatcacgaa aacagcaaag
    11581 ctatcttctg gaaagagggc gaaccgctga aaaagggcga cacgctggtg caggcgaacc
    11641 tggcaaagag cctggagatg attgctgaaa acggcccgga cgaattctat aaaggcacga
    11701 ttgcggaaca gatcgcccag gagatgcaga aaaacggtgg cttgatcact aaagaagatt
    11761 tagcggccta taaagcggtc gaacgcactc cgataagcgg cgattatcgc gggtatcagg
    11821 tttactccat gccaccgcca tcctccggcg ggatccatat cgtacaaatc ctcaatattc
    11881 tggaaaactt cgatatgaag aaatacggct ttggcagcgc cgatgcgatg caaatcatgg
    11941 cagaagcgga gaaatacgcc tacgccgacc gctcggaata tcttggcgac ccggattttg
    12001 tcaaagtccc gtggcaggca ctgaccaata aagcctatgc caaatccatt gccgatcaaa
    12061 ttgatatcaa caaagcgaag ccgtccagtg agattcgccc tggcaagctt gcgccttatg
    12121 agagtaatca aactacccat tactctgtag tggataaaga cggtaacgcg gtggcggtga
    12181 cctatacgct gaacaccacc ttcggtacgg gtattgtcgc gggcgagagc ggtattctgc
    12241 ttaataacca gatggatgat ttctccgcca aaccaggcgt accgaacgtt tacgggctgg
    12301 tgggcggtga tgccaacgcc gtcgggccga acaaacgccc gctgtcgtcg atgtcgccga
    12361 ccattgtggt gaaagacggt aaaacctggc tggttaccgg tagcccaggc ggtagccgga
    12421 tcatcactac agtgctgcaa atggtggtga atagcatcga ttatggcatg aacgtcgccg
    12481 aagcgaccaa tgcgccgcgt ttccaccatc agtggttgcc ggacgagctg cgtgtcgaaa
    12541 aagggtttag cccggatacg ctcaagctgc tggaagcaaa aggtcagaaa gtggcgctga
    12601 aagaggcgat gggcagtaca caaagcatta tggttgggcc ggacggtgag ttgtacggcg
    12661 catccgaccc gcgctcggtg gatgatttaa cggcggggta ctaaggttag cggccctctt
    12721 cgtgggaaga gggctatttt gtcagggcaa gccgaaggta gccttcttta tttcgtaatc
    12781 ctgtagatat tcttccagcg gctttgccgt tcccagagaa tgaatttcac cactgtcttt
    12841 atcaataata aaaggtgcgt taccagctaa gcgcgcggcc tcatctccag tttcgagaaa
    12901 ttctcgtgct tcgaaacaga aataccagcc ctggctaaag cgtccatgta gagtaatgac
    12961 gaccgggaga tctgcatcat caaggtaatg gttcgctttc gcgaatgcgt cgtgataagt
    13021 aatcataatt ataataaata ttattgtt
//
LOCUS       DAZGVQ010000139        13034 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000139 DAZGVQ010000000
VERSION     DAZGVQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.140"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            276..611
                     /gene="yjdM"
                     /locus_tag="ACHLAG_003961"
     CDS             276..611
                     /gene="yjdM"
                     /locus_tag="ACHLAG_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFP0657099.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            771..1214
                     /gene="yjdN"
                     /locus_tag="ACHLAG_003962"
     CDS             771..1214
                     /gene="yjdN"
                     /locus_tag="ACHLAG_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFP0657100.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1347..2135
                     /gene="phnC"
                     /locus_tag="ACHLAG_003963"
     CDS             1347..2135
                     /gene="phnC"
                     /locus_tag="ACHLAG_003963"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFP0657101.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQEHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2160..3176
                     /gene="phnD"
                     /locus_tag="ACHLAG_003964"
     CDS             2160..3176
                     /gene="phnD"
                     /locus_tag="ACHLAG_003964"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0657102.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3282..4061
                     /gene="phnE"
                     /locus_tag="ACHLAG_003965"
     CDS             3282..4061
                     /gene="phnE"
                     /locus_tag="ACHLAG_003965"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFP0657103.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4082..4807
                     /gene="phnF"
                     /locus_tag="ACHLAG_003966"
     CDS             4082..4807
                     /gene="phnF"
                     /locus_tag="ACHLAG_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFP0657104.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4808..5260
                     /gene="phnG"
                     /locus_tag="ACHLAG_003967"
     CDS             4808..5260
                     /gene="phnG"
                     /locus_tag="ACHLAG_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFP0657105.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETVIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5257..5841
                     /gene="phnH"
                     /locus_tag="ACHLAG_003968"
     CDS             5257..5841
                     /gene="phnH"
                     /locus_tag="ACHLAG_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFP0657106.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            5841..6905
                     /gene="phnI"
                     /locus_tag="ACHLAG_003969"
     CDS             5841..6905
                     /gene="phnI"
                     /locus_tag="ACHLAG_003969"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFP0657107.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQSPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6898..7743
                     /gene="phnJ"
                     /locus_tag="ACHLAG_003970"
     CDS             6898..7743
                     /gene="phnJ"
                     /locus_tag="ACHLAG_003970"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFP0657108.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7740..8498
                     /gene="phnK"
                     /locus_tag="ACHLAG_003971"
     CDS             7740..8498
                     /gene="phnK"
                     /locus_tag="ACHLAG_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFP0657109.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8603..9283
                     /gene="phnL"
                     /locus_tag="ACHLAG_003972"
     CDS             8603..9283
                     /gene="phnL"
                     /locus_tag="ACHLAG_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFP0657110.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9280..10416
                     /gene="phnM"
                     /locus_tag="ACHLAG_003973"
     CDS             9280..10416
                     /gene="phnM"
                     /locus_tag="ACHLAG_003973"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFP0657111.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10416..10973
                     /gene="phnN"
                     /locus_tag="ACHLAG_003974"
     CDS             10416..10973
                     /gene="phnN"
                     /locus_tag="ACHLAG_003974"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFP0657112.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10960..11394
                     /gene="phnO"
                     /locus_tag="ACHLAG_003975"
     CDS             10960..11394
                     /gene="phnO"
                     /locus_tag="ACHLAG_003975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFP0657113.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11396..12154
                     /gene="phnP"
                     /locus_tag="ACHLAG_003976"
     CDS             11396..12154
                     /gene="phnP"
                     /locus_tag="ACHLAG_003976"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFP0657114.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12301..12630)
                     /gene="yjdP"
                     /locus_tag="ACHLAG_003977"
     CDS             complement(12301..12630)
                     /gene="yjdP"
                     /locus_tag="ACHLAG_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFP0657115.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12699..13016)
                     /locus_tag="ACHLAG_003978"
     CDS             complement(12699..13016)
                     /locus_tag="ACHLAG_003978"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFP0657116.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
BASE COUNT         2869 a         3919 c         3662 g         2584 t
ORIGIN      
        1 ataaataacg tcttctgata cgtttaaaac gtcagaaaga taaaaatatc atgtgaataa
       61 aaaaaagaac aagtagagca ttaacattat cttaaataat aaatagaggc aaaaagatta
      121 ttttcttttt gcgtttcctt tcaaatgaaa acgatcgtcg tctaaaatca gcagtacccc
      181 cgacaaactc agggattttg tgtataattg cggccttttt cggcaatctg ccgttttttg
      241 gcgcttttgc cctgctgact tttgaggaaa tccacatgtc attaccacac tgcccaaaat
      301 gcaactccga atacacttac gaagataacg gcatgtacat ctgcccggaa tgtgcctacg
      361 aatggaacga cgcagaacct gcacaggaaa gcgacgagct gatcgttaaa gatgctaacg
      421 gcaatctgct ggctgacggc gacagcgtta ccatcattaa agatctgaag gtgaaaggta
      481 gctcttcgat gctgaaaatt ggcaccaaag tgaaaaacat ccgcctggtt gaaggcgacc
      541 ataacatcga ttgcaaaatc gacggttttg gtccgatgaa actgaaatct gagtttgtga
      601 aaaagaactg attgtattgt gatcggtaag ccggataagg cgctcgcgcc gcatccggca
      661 acgatgccga ctgcctgatg cgacgctgac cgcgtcttat caggcttctc tctcatctgt
      721 ataaatttcg aactacactt aactggcttc tcttaactga ggtcaccatc atgccgttaa
      781 gtccctacct ctcttttgcc ggtaactgtg ccgacgcaat tgcttattat caacgtacgt
      841 tgggcgctga actgctctat aaaatcagct tcggcgaaat gccaaaatca gcgcaggaca
      901 gcgccgagaa ctgcccttcc ggaatgcaat ttcccgatac cgccatcgcc cacgccaacg
      961 tgcgcattgc cggaagcgac atcatgatga gcgatgctat accgtcagga aaagccagct
     1021 actccggctt tacgctggtg ctcgattcgc aacaggtcga agaaggaaaa cgctggtttg
     1081 acaatcttgc cgctaacgga aaaatcgaaa tggcctggca ggaaactttc tgggcgcatg
     1141 gctttggcaa agtcaccgat aaatttggcg taccgtggat gattaatgtc gtcaaacaac
     1201 aaccaacgca ataacccgcc gggaggcccg ccctcccgca ctgtcatcga attcccgtta
     1261 actcttcatc tgttagtcac ttttaattaa ccaaatcgtc acaataatcc gccacgatgg
     1321 agccactttt ttagggaggc tgcatcatgc aaacgattat ccgtgtcgag aagctcgcca
     1381 aaaccttcaa tcagcatcag gcgctgcatg cggttgatct gaacattcat cacggtgaaa
     1441 tggtggctct gcttgggccg tcgggttccg gcaaatccac ccttttacgt cacttaagcg
     1501 gtttgattac cggcgataaa tccgccggca gccatatcga gctgctgggc cgcacagtcc
     1561 agcgcgaagg ccgtctggcg cgcgatatcc gcaaaagccg cgccaacacc ggctacatct
     1621 tccaacaatt caacctggtg aaccgcctga gcgtactgga gaacgtgctg attggcgcgc
     1681 tcggcagcac gccgttctgg cgcacctgtt ttagctggtt caccggcgag cagaaacagc
     1741 gcgcgttaca ggcgctgacc cgcgttggca tggtgcattt tgcccatcag cgcgtttcca
     1801 ccctctccgg cggacagcag cagcgtgtgg cgattgcccg cgcgctgatg cagcaggcga
     1861 aggtgattct ggccgatgaa cccatcgcct cgctggaccc ggaatccgcc cgcatcgtga
     1921 tggacaccct gcgcgacatc aatcagaacg acggcatcac cgtggtcgtc acgctgcatc
     1981 aggtggatta cgccctgcgc tactgcgaac gcatcgtcgc cctgcgccag gagcacgttt
     2041 tctacgacgg cagcagccaa cagtttgata acgaacgttt tgaccatctc taccgcagca
     2101 ttaatcgcat cgaagagaac gcgaaagctg cctgacatcc ccatcattga ggaaaacgaa
     2161 tgaacgctaa gataattgcc tcgctggcct tcaccagcat gttcagcctc agcaccctgt
     2221 taagcccggc acacgccgaa gagcaggaaa aggcgctgaa tttcggcatt atttcaacgg
     2281 aatcacagca aaacctgaaa ccgcaatgga cgccattctt acaggatatg gagaagaagc
     2341 tgggcgtgaa ggtgaacgcc ttctttgccc cagactacgc aggcattatc cagggaatgc
     2401 gcttcaataa agtggatatc gcctggtacg gcaacctgtc ggcaatggaa gcggtggatc
     2461 gcgccaacgg ccaggtcttc gcccagacgg tcgcggcgga tggatcgcca ggttactgga
     2521 gcgtgttgat cgtcaacaaa gatagtccga tcaacaacct gaacgatctg ctggcgaagc
     2581 ggaaagatct caccttcggc aatggcgatc ctaactccac ctctggcttc ctcgtccccg
     2641 gttactacgt ctttgccaaa aacaatatct ccgccagcga ctttaagcgc accgtcaacg
     2701 ctgggcatga aaccaacgcg ctggccgtcg ccaacaagca ggtggatgtt gccaccaaca
     2761 acaccgaaaa cctcgacaag ctgaaaacct ccgcgccaga gaagctgaaa gaactgaagg
     2821 tgatctggaa gtcgccgctg atcccaggcg atccgatcgt ctggcgtaaa aacctttccg
     2881 aaaccaccaa agacaagatc tacgacttct ttatgaacta cggcaaaacg ccggaagaaa
     2941 aagcggtgct ggaacgcctg ggctgggcgc cgttccgcgc ttccagcgac ctgcaactgg
     3001 tgccgattcg ccagctcgcg ctgtttaaag agatgcaggg cgtgaaaagc aataaaggac
     3061 tgaatgagca ggacaagctg gcaaaaacca ccgagattca ggcgcagctg gatgacctgg
     3121 accgcctgaa caacgcgtta agcgcgatga gttcggtgag taaagcggtg cagtaaatcg
     3181 taggtcggat aagacgcccc ggcgtcgcat ccgacaatgt gcaggcgttg atgccggatg
     3241 cggtgcaagc accttatccg gcctacagac cggagccaaa catgcaaacc atcaccatcg
     3301 ccccacccaa gcgtagctgg ttctcgcttc tgagctgggc cgttgttctc gccgtgttgg
     3361 tcgtctcgtg gcagggcgcg gaaatggccc cgctcacgct gattaaagac ggcggcaaca
     3421 tggcgacgtt cgccgccgac ttcttcccgc ccgatttcag ccagtggcag gattacctca
     3481 ccgaaatggc cgtcacgctg caaatcgccg tctggggcac ggcgctggcg gtggttctat
     3541 ccatcccctt cggcctgatg agcgccgaaa acctggtgcc gtggtgggtt taccagcccg
     3601 ttcgccgcct gatggacgcc tgccgcgcca ttaacgaaat ggtcttcgcc atgctgttcg
     3661 tggtcgccgt cggcctcggc ccgttcgctg gcgtgctggc gctgtttatc cacaccaccg
     3721 gcgtgctctc caaactgctt tccgaagcgg tggaagcgat tgaacccggc ccggtggaag
     3781 gcattcgcgc caccggcgcc aacaagctcg aagagatcct ctacggcgtg ctgccgcagg
     3841 tgatgccgct gctgatctcc tactccctct atcgcttcga atccaacgtc cgctcggcga
     3901 ccgtcgtcgg catggtcggt gcaggcggga tcggcgtcac cctgtgggaa gcgattcgcg
     3961 gtttccagtt ccaacaaacc tgcgccctga tggtgcttat catcgtcacg gtcagcctgc
     4021 tggatttcct ctctcaacgg ttgcgtaagc actttatctg ataagcgagg cattgatatc
     4081 tatgcacttg tctacacatc cgaccagcta cccaacacgc tatcaggaga tagccgcaaa
     4141 acttgagcag gagcttcgtc aacactaccg ctgcggcgac tatcttcccg ccgagcagca
     4201 actggcggcg cgctttgagg tgaaccgcca caccctgcgc cgcgccatcg accaactggt
     4261 ggaaaaaggc tgggtacagc gccgtcaggg cgtcggcgtg ctggtgctga tgcgcccgtt
     4321 cgattacccg ctcaacgccc aggcgcgttt tagccagaac ctgctggacc agggtagcca
     4381 tcccaccagc gaaaaactgc tgtcggtatt gcgccccgcg tccggtcacg tcgctgacgc
     4441 gctggggatt accgagggcg agaacgttat ccatttgcgc accctgcgtc gcgtcaacgg
     4501 cgtcgcgctc tgtttaatcg accactactt tgcggacctc accctttggc cgacgctgca
     4561 acgcttcgac agcggttcgc tgcacgattt tctgcgcgag caaaccggaa ttgcgctgcg
     4621 ccgcagccag acgcggatca gcgcccgtcg cgcccaggcc aaagagtgcc agcgtcttga
     4681 aattccgaat atgtcgccgc tgctgtgcgt gcgcaccctt aaccaccgtg acggtgaaag
     4741 cagcccggcg gagtactccg tcagcctgac gcgcgccgac atgattgaat tcactatgga
     4801 gcactgaatg cacgcagata ccgcgacccg ccagcactgg atgtccgtac tggcgcacag
     4861 ccaaccggct gaactggcag cccgcctgaa agcactgaac atcaccgccg actatgaggt
     4921 tatccgcgcc gccgaaaccg gcctggtgca gattcaggcg cggatgggcg gcaccggcga
     4981 acgctttttt gccggcgacg ccacgctcac ccgcgccgcc gtacgcctga ctgacggcac
     5041 gctcggctac agctgggtgc tggggcgcga caaacagcac gccgaacgct gcgcgctgat
     5101 tgacgcgctg atgcagcaat ctcgctattt tcaaaactta tcagaaaccg ttattgcccc
     5161 gctggacgct gaccgtatgg cacgcattgc cgcacgccag gccgaagtga acgccagccg
     5221 ggtcgacttc tttacgatgg ttcgcggaga caacgcatga ccctggaaac cgcttttatg
     5281 cttcccgtgc aggatgccca gcacagtttt cgtcgcctgt taaaggcgat gagcgagccg
     5341 ggcgtgattg tcgccctgca tcagctcaaa cgcggctggc agccgctgaa tatcgccacc
     5401 accagcgtgc tgctgacgct ggccgataac gacacgccgg tgtggcttgc cacgccatta
     5461 agtaacgata tcgtcaatca gagcctgcgt tttcatacca acgcgccgct ggtcagccag
     5521 ccggaacagg cgaccttcgc ggtgacggat gaggcgattt ccagcgaaca gctcaacgcc
     5581 ctttccaccg gcaccgccgt tgcgccggaa gcgggcgcga cgctgatttt acaggtcgcc
     5641 agcctgagcg gcggacgcat gttgcgtctc accggcgcgg gcattgccga agaacgaatg
     5701 atcgctccgc agctgccgga gtgcattctg cacgaactca ccgagcgccc gcacccgttc
     5761 ccgctcggca tcgacctgat cctgacctgc ggcgagcgcc tgctggctgt tccgcgaacc
     5821 actcatgtgg aggtgtgctg atgtacgttg ccgtgaaagg gggcgaaaag gcgatcgacg
     5881 ccgcccacgc cctgcaagag agccgacgcc ggggcgatac cgatttgccc gaactgagcg
     5941 tcgcccagat tgaacagcag cttaacctcg cggtagatcg cgtgatgacc gaaggcggca
     6001 ttgccgaccg cgaactggcg gcgctggcgc tgaaacaggc cagcggcgat aacgtcgaag
     6061 cgattttcct gctacgcgcc taccgcacca cgctggcgaa gctggcggta agcgagccgc
     6121 tcgacaccac ggagatgcgt ctcgaacggc gtatttcagc ggtttataaa gatattcccg
     6181 gcggtcagtt gcttggccca acctacgact acacccatcg cctgctcgat ttcaccctgc
     6241 tggcaaacgg cgaagcgccg acgctgacca ccgcagacag cgaacaatcc ccgtcgccgc
     6301 acgttttcag cctgctggcg cgtcaggggc tggcgaagtt tgaagaggat agcggcgcac
     6361 agccggatga catcacccgc acgccgccgg tttacccctg ttcacgttct tcccgtttgc
     6421 agcagttgat gcgcggcgac gaaggctatt tgctggcgct ggcctactct acccagcgcg
     6481 gttacggacg caatcacccg ttcgcgggcg aaatccgcag tggttacatc gacgtgtcga
     6541 ttgtgccgga agagctggga tttgcggtga acgtcggcga actgctgatg accgagtgtg
     6601 aaatggtcaa cggttttatt gacccgccgg atgagccgcc gcacttcacg cgcggctacg
     6661 ggctggtgtt cggcatgagc gagcgcaaag cgatggcgat ggcgctggtt gaccgtgcgt
     6721 tgcaggctcc ggagtacggc gagcacgcga caggcccggc gcaggatgaa gagttcgtgc
     6781 tggcgcatgc cgacaacgtc gaagccgcag gctttgtctc gcacctcaaa ctcccgcact
     6841 acgtcgattt ccaggccgaa ctggagctac tcaaacgtct gcaacaggag cagaaccatg
     6901 gctaatctga gcggctacaa ctttgcctac ctcgacgagc agaccaaacg catgatccgc
     6961 cgcgccatct taaaagcggt ggcgatcccc ggttatcagg tgccgtttgg cgggcgcgag
     7021 atgcccatgc catacggctg gggaaccggc ggcatacagc tcaccgccag cgtgattggc
     7081 gaaagcgacg tgttgaaggt gattgaccag ggcgcggatg acaccaccaa cgccgtgtcg
     7141 attcgcaact tctttaagcg cgtgaccggg gtgaacacca ctgaacgtac ggatgatgcg
     7201 acgcttatcc agacgcgcca ccgcattccc gaaacgccgc tgacggaaga tcagatcatt
     7261 atcttccagg tgccgatccc cgaaccgctg cgctttatcg agccgcgcga aacggaaacc
     7321 cgcaccatgc acgcgctgga agagtacggc gtgatgcagg tgaaactgta tgaagatatc
     7381 gcccgcttcg gtcatatcgc cactacctac gcctatccgg tgaaggtgaa cgggcgctac
     7441 gtaatggacc cgtcgccgat cccgaaattc gataacccaa aaatggacat gatgcccgcc
     7501 ctgcaactgt tcggcgcggg gcgcgagaag cgcatctatg cggtgccgcc gtttacccgc
     7561 gtggaaagtc tcgatttcga cgatcacccg ttcaccgttc agcagtggga tgagccatgc
     7621 gccatctgcg gatcgaccca cagctatctt gatgaagtgg tgctggatga cgccggaaac
     7681 cgcatgtttg tctgctccga taccgattat tgccgccaac agagcgaggc aaaaaaccaa
     7741 tgaatcaacc gttactttcg gtcaataacc tgacccacct ttacgcgccg ggcaaaggct
     7801 ttagcgatgt ctcttttgat ttatggccgg gggaagtgct gggcattgtc ggggagtccg
     7861 gctccgggaa gaccacgctg ctgaaatcaa tctccgcgcg cctgacgccg cagcaggggg
     7921 agattcacta tgagagccgt tcgctgtacg caatgagcga ggccgaccgc cgtcgcctgc
     7981 tgcgcaccga atggggcgta gtgcatcagc atccgctcga cggcctgcgc cgccaggtgt
     8041 cggcaggcgg caatatcggc gagcggctga tggcgaccgg ggcacgtcat tacggcgata
     8101 ttcgtgccac cgcgcagaag tggctggaag aggtggaaat tcccgccaac cggatcgacg
     8161 acctgccgac caccttttcc ggcggcatgc agcaacgttt gcagatcgcc cgcaacctgg
     8221 tgacgcatcc gaagctggtg tttatggacg agccgaccgg cggactggat gtgtcggtac
     8281 aggcacgcct gctcgacctg ctgcgcggcc tggtggtgga gctgaacctc gcggtagtga
     8341 ttgtcaccca cgatttaggc gtcgcccgcc tgctggcgga ccgtttgctg gtgatgaagc
     8401 aggggcaagt ggtggagagt gggttaaccg accgcgtgct cgacgacccg caccatccgt
     8461 atacacaact gctggtgtca tcggttttgc agaattgaat gcaacaccgc gcacaatcgg
     8521 tcccctcgcc ccttcgggga gagggttagg gtgaggggca atgcgcgcac cgctccccaa
     8581 tatgcgaacc acgaggcgaa aaatgattaa cgtacaaaac gtcagtaaaa ccttcatcct
     8641 gcaccagcaa aacggcgtgc gcctgcccgt cttacaacgc gcctcgctca ccgtcaacgc
     8701 aggcgaatgc gtggtgctcc acggtcactc cggcagcggc aaatcaaccc tgctgcgctc
     8761 gctgtacgcc aactatctac ccgacgaagg tcaaatccag atcaaacacg gtgacgagtg
     8821 ggtagacctg gtcaccgcgc cagcgcgcaa agtggtggag atccgcaaaa ctaccatcgg
     8881 ctgggtgagc cagtttctgc gcgtcatccc gcgtatctca gcactggaag tggtgatgca
     8941 gccgctgctc gatacaggcg ttccgcgtga agcctgcgcc gctaaagccg cgcgtcttct
     9001 cacccgcctg aacgtgccgg aacgcctgtg gcacctggca ccatcgacct tctccggcgg
     9061 cgaacagcag cgcgtcaaca tcgcccgcgg ctttatcgtc gactacccca ttctgctgct
     9121 cgacgaacct accgcctcgc tggacgccaa aaacagcgcc gcggtggtgg aactgattca
     9181 cgaagccaaa gcccgtggcg cagccatcgt cggcatcttc catgacgaag ctgtacgtaa
     9241 tgacgtcgcc gaccgcctgc acccaatggg agcctcttca tgattatcaa taacgttaag
     9301 ctggtgctgg aaaacgaggt ggtgcacggt tcgctggagg tgcaggatgg cgaaatccgc
     9361 gcctttgccg aaagccagag ccgcctgcca gaagcgatgg acggcgaagg tggctggcta
     9421 ctacccggcc tgattgagct gcataccgac aacctcgata aattcttcac cccgcgcccg
     9481 aaagtcgact ggcccgccca ttcggcgatg agcagccacg acgcgctgat ggtggcaagc
     9541 ggcatcacca ccgtgctgga cgcggtggcg attggcgacg tgcgcgacgg cggcgatcgg
     9601 ctggagaatc tggagaagat gatcaacacc atcgaagaga cgcagaaacg cggcgtcaac
     9661 cgcgccgagc accgcctgca cctgcgctgc gaactgccgc atcacaccac actgccgctg
     9721 tttgaaaaac tggttcagcg cgaaccggtg acgctggtgt cgctgatgga ccactcaccg
     9781 ggccagcgcc agttcgctaa ccgcgagaag tatcgcgaat attatcaggg caaatactcc
     9841 ctcaccgatg cgcagatgca gcagtacgaa gaagagcaat tggcgctcgc cgcacgctgg
     9901 tcgcagccga atcgcgaatc catcgccgcc ctgtgccgcg cgcgacaaat tgcgctcgcc
     9961 agccacgatg acgccacgca cgcccacgtt gccgaatccc accaacttgg cagcgtgatc
    10021 gccgaatttc ccaccacgtt cgaagcggcg gaagcctcgc gtaagcatgg catgaacgtg
    10081 ctgatgggtg cgccaaatat cgtgcgtggc ggctcgcact ccggcaatgt ggcggccagt
    10141 gaactggcgc agcttggtct gctggatatc ctctcttccg actactaccc cgccagcctg
    10201 ctggatgcgg cgttccgcgt cgccgatgac gagagcaacc gctttacgct accgcaggcg
    10261 gtgaggctgg tgaccaaaaa tccggcgcag gcgctgaatc ttcaggatcg cggggtgatt
    10321 ggtgagggta aacgcgctga cctggtgctg gcgcatcgcc agggcaatca cattcatatc
    10381 gaccacgtct ggcgtcaggg taaaagggtg ttctgatgat gggaaaactg atttggttaa
    10441 tggggccgtc cggctccggg aaagacagcc tgctggcgga actccgcctg cgggaacaaa
    10501 cgcagttact ggtggcgcat cgctacatca cgcgcgacgc cagcgccgga agtgaaaacc
    10561 atatcgccct gagtgagcgg gagtttttta cccgcgcggg gcaaaacctg ctggccttaa
    10621 gctggcacgc taacggtctg tattatggcg tcggcatcga gattgacctc tggctgcacg
    10681 ctggattcga cgtagtcgtc aacggttcac gcgcgcattt gccgcaggca cgggcgcgct
    10741 atcaatcggc gctgctgccc gtctgtttac aggtttcgcc ggagatcctg cgccagcgcc
    10801 tggaaaaccg tggccgtgaa aacgccagtg aaattaacgc ccgcctggcg cgcgccgccc
    10861 gctatactcc acaggattgc catacgctca acaatgacgg cagcctgcgc cagtcggtcg
    10921 acacgctgct gacgctgatc catcagaagg agaaacacca tgcctgcttg tgagcttcgc
    10981 ccggccacgc agtacgatac cgacgcggtt tacgcgctga tttgtgagct aaaacaggcg
    11041 gagtttgacc accatgcgtt tcgcgtgggc tttaacgcca acctgcgcga cccaaacatg
    11101 cgctaccatc tggcgctgct ggatggcgaa gttgtcggca tgatcggcct gcatttgcag
    11161 tttcatctgc atcatgtcaa ctggatcggc gaaattcagg aactggtggt gatgccgcag
    11221 gcgcgcggtc tgaacgtcgg cagtaaatta ctggcatggg cagaagaaga agcccgccag
    11281 gccggggccg aaatgaccga gctttccacc aacgtgaagc gccacgacgc acaccgtttt
    11341 tacctgcgtg aaggctacga gcagagccac ttccgcttca ccaaggcgct gtaacatgag
    11401 cctgaccctc acgctcaccg gcactggcgg cgcacagggc gttccggcat ggggctgcga
    11461 gtgcgcggcc tgcgccagag cgcggcgctc gccgcagtat cgccgccagc catgcagcgg
    11521 cgtagtgaag tttaacgacg cgatcaccct gatcgacgcc gggcggcacg atctcaccga
    11581 tcgctggtcg cctggatcgt tccagcagtt tttgctgacg cactaccata tggatcacgt
    11641 ccaagggcta tttccgctac gctggggcgt gggcgatgtg atcccggtct acggcccgcc
    11701 ggatgaacag ggctgcgacg atctgtttaa acatccgggc ctgcttgatt tcagccacac
    11761 agtggaaccg tttgtggtgt ttgatttgca ggggttgcag gtcacgccgc tgccgcttaa
    11821 ccactcaaaa ctgaccttcg gttatctgct ggaaacggca catagccggg tggcgtggct
    11881 gtctgacacc gctggcctgc cggaaaaaac gctgaaattt ttactcaata atcatccgca
    11941 ggtgatggtg attgattgca gtcacccgcc gcgcgcggat gcaccgcgta atcactgtga
    12001 tttaaatacc gtgcttgcgc tgaatcaggt tatccgctcg ccacaggtga ttctgaccca
    12061 tatcagccac cagtttgatg cgtggctgat ggaaaacgca ctaccgtcag ggtttgaggt
    12121 ggggtttgat gggatggaga ttggggtggc gtgatgagag ggaatgtgcg cgctggcccc
    12181 ctcaccctaa ccctctcccc agaggggcga ggggaccgat tgtgctcaat attgaatatt
    12241 gcgctcgttt tctccctctc cccattgggg tgaggggcga tgcctgctcc atacccaacc
    12301 tcatcgccca tactcatctt ccattctccg ctcttcatcc tccagttgcc gacgctcctg
    12361 atcaagctgg cgctggcgat cgtccagctg cctgcggcga tcttcaaact ggcggcggcg
    12421 gtcgtcatat tgtctgcgcc gatcgtcgct cacttcacgc tgccagccgt cgtcgcgcga
    12481 atcttcatag tctcgcccac ggtcagggtt ataagcgtca ttaatcgcct gctgaatatt
    12541 accaatagta tcgtcgataa tatcagcctg ggccggaacg gtggacagcg tgagcagggt
    12601 gaataaaaga aatagcggaa agcgtttcat tagccaacct caaaaagaaa ctctatccac
    12661 attagtcatt actcatccat gcaagtagtg gatgaatctc aatttctccg ctgctctatt
    12721 gccgtaatcg cctccacgcg ttgttgatga cgaccgcctt cgtactgtgc gcccagccac
    12781 gcatccacaa tcatttttgc cagttcgagg ccaaccactc gagaaccaaa agccagtgca
    12841 acttcactgg cataatgcgg ataatcagtg cgctctgacg acctggtgtc tttatcaatc
    12901 acctcactca ttgaccatga ataaaaggct ggcacagcaa cgcagaatcc tgcggtgcca
    12961 gcgacatgac cgcccattgc tccggtacgt tttttaaaaa gaaacaccgg ttgcattgat
    13021 aaattgtgct cagt
//
LOCUS       DAZGVQ010000140        13013 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000140 DAZGVQ010000000
VERSION     DAZGVQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.141"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            384..1097
                     /gene="aphA"
                     /locus_tag="ACHLAG_003979"
     CDS             384..1097
                     /gene="aphA"
                     /locus_tag="ACHLAG_003979"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFP0657117.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPVT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            1208..1624
                     /gene="yjbQ"
                     /locus_tag="ACHLAG_003980"
     CDS             1208..1624
                     /gene="yjbQ"
                     /locus_tag="ACHLAG_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFP0657118.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            1628..1984
                     /gene="yjbR"
                     /locus_tag="ACHLAG_003981"
     CDS             1628..1984
                     /gene="yjbR"
                     /locus_tag="ACHLAG_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP0657119.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(2019..4841)
                     /gene="uvrA"
                     /locus_tag="ACHLAG_003982"
     CDS             complement(2019..4841)
                     /gene="uvrA"
                     /locus_tag="ACHLAG_003982"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP0657120.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            5095..5631
                     /gene="ssb1"
                     /locus_tag="ACHLAG_003983"
     CDS             5095..5631
                     /gene="ssb1"
                     /locus_tag="ACHLAG_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFP0657121.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            complement(5730..6011)
                     /gene="yjcB"
                     /locus_tag="ACHLAG_003984"
     CDS             complement(5730..6011)
                     /gene="yjcB"
                     /locus_tag="ACHLAG_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFP0657122.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            6441..8027
                     /gene="pdeC"
                     /locus_tag="ACHLAG_003985"
     CDS             6441..8027
                     /gene="pdeC"
                     /locus_tag="ACHLAG_003985"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFP0657123.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            complement(8030..8353)
                     /gene="soxS"
                     /locus_tag="ACHLAG_003986"
     CDS             complement(8030..8353)
                     /gene="soxS"
                     /locus_tag="ACHLAG_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFP0657124.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            8439..8903
                     /gene="soxR"
                     /locus_tag="ACHLAG_003987"
     CDS             8439..8903
                     /gene="soxR"
                     /locus_tag="ACHLAG_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFP0657125.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            9449..10798
                     /gene="ghxP"
                     /locus_tag="ACHLAG_003988"
                     /gene_synonym="yjcD"
     CDS             9449..10798
                     /gene="ghxP"
                     /locus_tag="ACHLAG_003988"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFP0657126.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            10950..12599
                     /gene="yjcE"
                     /locus_tag="ACHLAG_003989"
     CDS             10950..12599
                     /gene="yjcE"
                     /locus_tag="ACHLAG_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFP0657127.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(12753..>13013)
                     /locus_tag="ACHLAG_003990"
     CDS             complement(12753..>13013)
                     /locus_tag="ACHLAG_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP0657128.1"
                     /translation="NCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSD
                     TLVTLQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
BASE COUNT         3044 a         3193 c         3408 g         3368 t
ORIGIN      
        1 agtaataaaa ttagtcacca ttgtaggata gggttctggt caatcaggaa tcatctttat
       61 aaacttcgat tgtttttgta atgctgtatc attaagttca ttaaatcgta cagcagataa
      121 atgttctatc aaatttcgct catttgcaga ggattcatca taataaacgt aaaattaatg
      181 tatccttaca tcgagtaata aacatttttt atacaaaaaa agacaggaac gtatttactg
      241 ggttaaacat aatcatcctg cttttcatca caaaaaccgc agataatcct tcctttcccc
      301 ggcagctggc gttatggtca gatggttttt gcaacaaatc tcacaataaa aagtttcaac
      361 atactgacta tttagggaaa aatatgcgca agatcacaca ggcaatcagt gccgtttgct
      421 tattgttcgc tctaaacagt tccgctgttg ccctggcctc atctccttca ccgcttaacc
      481 ctgggactaa cgttgccagg cttgctgaac aggcaccaat tcattgggtt tcggtcgcac
      541 aaattgaaaa tagcctcgca gggcgtccgc caatggcggt ggggtttgat atcgatgaca
      601 cggtactttt ttccagcccg ggcttctggc gcggcaaaaa aaccttctca ccagaaagcg
      661 aagattatct gaaaaatcct gtgttctggg aaaaaatgaa caatggctgg gatgaattca
      721 gcattccaaa agaggtcgct cgccagctga ttgatatgca tgtacgccgc ggtgacgcga
      781 tcttctttgt gactggtcgt agcccgacga aaacagaaac ggtttcaaaa acgctggcgg
      841 ataattttca tattcctgtc accaacatga atccggtgat ctttgcgggc gataaaccag
      901 ggcaaaatac aaaatcgcaa tggctgcagg ataaaaatat ccgaattttt tatggcgatt
      961 ccgataacga tattaccgcc gcacgcgatg tcggcgctcg tggtatccgt attctgcgcg
     1021 cctccaactc tacctacaaa cccttgccac aagcgggtgc gtttggtgaa gaggtgatcg
     1081 tcaattcaga atactgacag agcgggagag cgtgacgctc tcccgaatgc tgttttttta
     1141 atcacacctt tatcctttcg ctgtcttgct gcaaactgat taagagagtt ttatcaagga
     1201 gcagcacatg tggtatcaaa agacgctcac gcttagcgcc aaatctcgtg ggtttcatct
     1261 ggtaacggat gaaattctga atcagctggc tgatatgccg cgcgttaaca tcggcttact
     1321 gcatctgttg ctgcaacata cctccgcctc tctgacactt aatgagaact gcgatcccac
     1381 cgtacgccac gacatggagc gttttttcct ccgcaccgtt cccgacaacg gaaattatga
     1441 gcatgactat gagggagcag acgatatgcc ttctcatatc aaatcctcaa tgctgggaac
     1501 atcgcttgta ttgccggtac ataaagggcg tattcagacc ggcacctggc aaggcatctg
     1561 gctgggggaa catcgcatcc acggcggatc gcgtcgcatc atcgcgacac tacaagggga
     1621 gtaaaaaatg accatttcgg agttgctaca atattgcatg gcaaaaccag gcgcagaaca
     1681 gagcgtgcat aacgactgga aagcgacgca gatcaaagtg gaagatgtac tgtttgcgat
     1741 ggtgaaagaa gtagaaaatc gcccagctgt ttcgctgaaa accagcccgg agctggcgga
     1801 gctgctacgt cagcagcaca gcgatgtgcg tcctagccgc catctgaata aagcgcactg
     1861 gagcaccgtg tatctcgacg gttcgctgcc ggattcgcaa atctattatc tggtggatgc
     1921 gtcttatcag caggcagtga atttactacc ggaagaaaag cgtaaattgc tggtacaact
     1981 ctgaaaggaa aaggccgctc agaaagcggc cttaacgatt acagcatcgg cttgaggaag
     2041 cgtgccgtat gcgaagcttc gcactccgcg acggtttctg gcgtaccgga gacgaggatt
     2101 tcgccgccgc cactgccgcc ttctggtccc aggtcgacaa tccagtcagc ggttttgatc
     2161 acgtcgagat tgtgctcaat caccacaatg gtattgccct gatcgcgcag tttatgcagc
     2221 acgtcgagga gttgctgaat atcggcgaag tgcagaccgg tggtcggctc gtcgagaata
     2281 tacagcgtct gcccggtgcc gcgttttgac agctcacgcg ccagcttcac gcgctgggct
     2341 tcaccaccag aaagtgtggt tgcggactgc cccaggcgaa tgtacgtcag accaacgtcc
     2401 atcaacgttt gcagcttacg cgccagagct ggcaccgcat caaagaactc acgcgcctct
     2461 tcgatggtca tatccagcac ttcgtggatg gttttgcctt tgtacttaat ttccagcgtt
     2521 tcacggttat agcgtttacc tttgcactga tcgcacggca cgtagatatc cggcaggaag
     2581 tgcatctcca ctttgatcac accgtcgccc tgacaggctt cgcagcgccc gccacggacg
     2641 ttaaagctga aacgtcctgg cgtataacca cgcgcacggg attccggtac gcccgcaaac
     2701 agttcgcgca caggtgtaaa cacgccggta taggtcgccg ggttagaacg tggagtacga
     2761 ccaattgggc tttggtcgat atcgatcact ttatcgaaat gctccagccc ctgaatatcg
     2821 cgatacggtg ccggttcggc gatggtcgca ccattcaact ggcgttgggc aatcgggaac
     2881 agtgtgtcgt taatcagcgt cgatttaccg gaacctgaaa cccctgtgat gcaggtaaac
     2941 agaccgactg gcagcgtgag cgtcacgtcc ttcaggttgt tgccgcgtgc gcctgtcagc
     3001 ttcagcactt tttccggatt tgccggaacg cgtttcttcg gcacttcaat cttgcgttta
     3061 ccgctcatgt actgcccggt caacgactcc ggcaccgcca taatcgcttc cagcggacct
     3121 tctgcgacca cttcaccgcc gtgaacacct gcgcccgggc caatgtcgat cacatggtca
     3181 gcggcgcgaa ttgcgtcttc gtcgtgctcc accacaatca cggtattacc gagatcgcgc
     3241 agatggataa gcgtacccaa caggcgctcg ttatcgcgct ggtgcaggcc gatagatggc
     3301 tcgtccagca cgtacataac gccaaccagg cccgcaccaa tctggctcgc cagacggata
     3361 cgctgggctt caccgccgga aagcgtttct gccgagcggg aaagcgtcag gtaattcagg
     3421 ccgacgttaa cgaggaattt caaacgatcg ccgatctctt taaggatttt ttccgcaatc
     3481 ttcgcccgtt gaccagcgag tttgagattg ttgaagaatt ccatcgcatg accgatgctc
     3541 atgtcggaga tagcaggcag cggcgtattc tcgacataaa cgtggcgcgc ttcccgacgc
     3601 agacgcgtcc cttcgcagct ggcgcatggg cgattgctga taaacttggc taattcttca
     3661 cgtaccgcac tggattctgt ctctttataa cggcgctcca tattgtgcag cacgccttcg
     3721 aacggatgac gacggatgga ggtatcgcca cgatcgttca tgtatttgaa ttcaatgttt
     3781 tctttgccag aaccgtacaa caccacttta tgcacgttcg cgctcaggct gccccacggc
     3841 gcttcgacgt cgaacttata gtgatctgcc agcgatttca gcatctggaa gtaatagaag
     3901 ttgcggcgat cccagccacg gatcgcaccg ccagccagtg acagctcggg gttttggatc
     3961 acgcggtcag gatcgaaata ttgctgtacg ccaaggccgt cacaggttgg gcaggcacct
     4021 gccgggttgt taaacgaaaa cagtcgcggc tccagttcac gcatactgta gccgcaaatt
     4081 gggcaggcga agttggcgga gaacagcagc tcttccgctt tcgggtcgtc catatccgcc
     4141 actaccgctg taccaccgga aagctccagc gcggtttcaa acgactccgc aagacgttgg
     4201 gtaagatcgt cacgcacctt gaagcgatca accaccactt caatggtatg tttcttttgc
     4261 agttccagtt tcggcggatc ggaaagatcg cagacttcgc catcaatacg agcacggatg
     4321 taaccctggc ttgccaggtt ctccagcgtt ttggtgtgtt cgcctttgcg ctctttaatg
     4381 attggcgcga gcagcatcag acgcttgcct tccggctgcg acagcacgtt atccaccatc
     4441 tggctgacgg tttgcgccgc cagcgggacg tcgtggtccg gacagcgcgg ttcgccgacg
     4501 cgggcgaaca acaaacgcaa atagtcgtgg atttcggtga ttgtccccac cgtagaacgc
     4561 gggttatgag acgtcgattt ctgctcaatt gagatggcag gagaaagccc ctcaatatga
     4621 tcgacgtccg gcttttccat cagtgacaga aactgccgcg cgtaggcgga aagggattca
     4681 acgtaacggc gctgcccttc ggcatataag gtgtcgaaag cgagcgagga tttgccagaa
     4741 cccgaaagcc cggtcacgac aatgagcttg tcgcggggga taacgaggtt gatgtttttg
     4801 agattatggg tgcgggcgcc ccgaacttcg atcttatcca ttcacctttc ccggattaaa
     4861 cgcttttttg cccggtggca tggtgctacc ggcgatcaca aacggttaat tatgacacaa
     4921 attgacctga atgaatatac agtattggaa tgcattaccc ggagtgttgt gtaacaatgt
     4981 ctggccaggt ttgtttcccg gaaccgaggt cacaacatag taaaagcgct attggtaatg
     5041 gtacaatcgc gcgtttacac ttattcagaa cgattttttt caggagacac gaacatggcc
     5101 agcagaggcg taaacaaggt tattctcgtt ggtaatctgg gtcaggaccc ggaagtacgc
     5161 tacatgccaa atggtggcgc agttgccaac attacgctgg ctacttccga atcctggcgt
     5221 gataaagcga ccggcgagat gaaagaacag actgaatggc accgcgttgt gctgttcggc
     5281 aaactggcag aagtggccag cgaatatctg cgtaaaggtt ctcaggttta tatcgaaggt
     5341 cagctgcgta cccgtaaatg gaccgatcaa tccggtcagg atcgctacac cacagaagtc
     5401 gtggtgaacg ttggcggcac catgcagatg ctgggtggtc gtcagggtgg tggcgctccg
     5461 gcaggtggta atatcggtgg tggtcagccg cagggcggtt ggggtcagcc tcagcagccg
     5521 cagggtggca atcagttcag cggcggcgcg cagtctcgcc cgcagcagtc cgctccggca
     5581 gcgccgtcta acgagccgcc gatggacttt gatgatgaca ttccgttctg atttgtcatt
     5641 aaaacaatag gttatattgt tttaaggtgg atgattaaag catctgccag ccataaaaaa
     5701 gaagcctccg ttatggaggc ttctacgtat caggtcaaaa tcattggcca ttgtggcggt
     5761 gtctgattca tctcttctat catcaccgac tcgatgttgt gccagatagc ggtgatgtcc
     5821 gccattgtga tgccaagcaa ccccattgcg aaccaacagg cggaaacaac gcccagaccg
     5881 ctgctgatca ccgcaagccc gacataatca gaccgacgaa aacggatgtt gagtgtgctg
     5941 gccagaaaca tcattacggc actaagaagt tgccagcgaa gaagaaccac gccagtggtg
     6001 agggtagcca tcaaacaatt cctctgaaaa gagccgatgc cctggacagc gcggcgttgt
     6061 tcacgggagg cgtggtacac tctggctatc gcggggcttg cagaacacaa aaatgaaaca
     6121 ctctgtttgt ttcattaatt ttgtgaacta tatcacaatt gattgtttgt tagccatatt
     6181 aggccgtgac ttttattgct gtacagatta tgtggttttt cagtggcatt aagggcatat
     6241 cttcccgccg cctctgcatt cctgtaggaa attaattttg aatatcaatg aattattttc
     6301 atccaggtga cgattagaaa ggtatcaatt tcaaatcagg caaaagtgct atttataccg
     6361 taagatttat ctaaagacgt cggtacccag ggttttcacc ttgcaatggc cgggtataaa
     6421 caggcaggaa attgatagca atgagtcatc gtgcacgaca ccaattactg gcgttgccgg
     6481 gcattatctt tttagttctc tttcccatca ttctttcgct atggattgcc ttcctttggg
     6541 caaaatcaga agtgaataat cagctccgaa cctttgctca actggcactg gataaatccg
     6601 agctggtcat tcgccaggca gatttagtga gcgatgcagc tgaacgctat caggggcaag
     6661 tttgcactcc agcccatcaa aagcgaatgt tgaatattat tcgtggctat ctttatatta
     6721 atgaattgat ctatgcccgt gataaccatt ttttatgctc atcgctgata gcgcctgtaa
     6781 acggctatac gattgcaccg gccgattata agcgtgaacc taacgtttct atctattatt
     6841 accgcgatac gccttttttc tctggctata aaatgaccta tatgcagcgg ggaaattatg
     6901 tggcggttat caaccctctc ttctggagtg aagtgatgtc tgatgacccg acattgcaat
     6961 ggggtgtgta tgatacggtg acgaaaacct ttttctcgtt aagcaaagag gcctcggcag
     7021 caacgttttc tccgctgatt catttgaagg atttaaccgt acaaagaaat ggctatttat
     7081 atgcgacagt ttattcgaca aaacgcccaa ttgcagccat tgttgcgact tcatatcaac
     7141 gtcttataac ccatttttat aatcatctta tttttgcgtt gcccgccggt attttgggga
     7201 gtcttgttct gctattactc tggctacgta ttcgacaaaa ctatttatct cccaaacgta
     7261 aattgcaacg cgccctcgaa aaacatcaac tttgtcttta ttaccagcca ataatcgata
     7321 tcaaaacaga aaaatgtatc ggcgctgaag cgttgttacg ttggcttggt gagcaggggc
     7381 aaataatgaa tccggcagag tttattccgc tggcagaaaa ggaggggatg atagaacaga
     7441 taactgatta tgttattgat aatgtcttcc gcgatctggg cgattacctg gcaacacatg
     7501 cagatcgcta tgtttctatt aacctgtcgg cctccgattt tcatacgtca cggttgatag
     7561 cgcgaatcaa tcagaaaaca gagcaatacg cggtgcgtcc gcagcaaatt aaatttgaag
     7621 tgactgaaca tgcatttctt gatgttgaca aaatgacgcc gattattctg gctttccgcc
     7681 aggcaggtta cgaagtggca attgatgatt ttggtattgg ctactctaac ttgcataacc
     7741 ttaaatcatt gaatgtcgat attttgaaaa tcgataaatc gtttgttgaa acgctgacca
     7801 cccacaaaac cagtcatttg attgcggaac acatcatcga gctggcgcac agcctggggt
     7861 taaaaacgat cgctgaaggc gtcgaaactg aggagcaggt taactggctg cgcaaacgcg
     7921 gcgtgcgcta ttgccaggga tggttttttg cgaaggcgat gccgccgcag gtgtttatgc
     7981 aatggatgga gcaattaccc gcgcgggagt taacgcgcgg gcaataaaac tacaggcggt
     8041 ggcgataatc gctgggagtg cgatcaaact gccgacggaa aacgcgggag aaggtctgct
     8101 gcgagacata acccaggtcc attgcgatat caaaaatcgg acgctcggtg gtgcgcaact
     8161 caacggcggc cagtaacagg cgacgttggc gaatgtaatc gccaagcgtc tgatgcgtca
     8221 ccgtgcggaa cattcgttgc aagtaccact ttgaatagcc tgattttttt gcgactacat
     8281 caatgttaag cggctggtca atatgctcgt caatccatgc gataagatcc tgaataattt
     8341 tctgatggga cataaatctg cctcttttca gtgttcagtt cgttaattca tctgttgggg
     8401 agtataattc ctcaagttaa cttgaggtaa agcgatttat ggaaaagaaa ttaccccgca
     8461 ttaaagcgct gctaaccccc ggcgaagtgg cgaaacgcag cggtgtggcg gtatcggcgc
     8521 tgcatttcta tgaaagtaaa gggttgatta ccagtatccg taacagcggc aatcagcggc
     8581 gatataaacg tgatgtgttg cgatatgttg caattatcaa aattgctcag cgtattggca
     8641 ttccgctggc gaccattggt gaagcgtttg gcgtgttgcc cgaagggcat acgttaagtg
     8701 cgaaagagtg gaaacagctt tcgtcccaat ggcgagaaga gttggatcgg cgcattcata
     8761 ccttagtggc gctgcgtgat gaactggacg gatgtattgg ttgtggctgc ctttcgcgca
     8821 gtgattgccc gttgcgtaac ccgggcgacc gcttaggaga agaaggtacc ggcgcacgct
     8881 tgctggaaga tgaacaaaac taaagcgcca caagggcgct ttagtttgtt ttccggtctt
     8941 tgtctttctc tctatcccgc tggtacacag gagggtttcc cccgacgtca acacacctca
     9001 ttcgagcacg tggtggaggt tccggttggt gttgatgctt taattgtatg tcaccgacgt
     9061 ttcttcgcca gtgtaaaagt atacttttta accgcaatat ttttgtcatc tcagacgatt
     9121 ttttatcgca atcctgaacg gtatacggct cgataacgct gcaatcttgc gcaccgacga
     9181 taacgtttgc gcatcaattg cctgtttttt catcgtcaag acaataaaag agaaaaaagc
     9241 agcaaacttc ggttgaaaaa gccgctatga tcgccggata atcgtttgct ttttttacca
     9301 cccgttttgt atgcgcggag ctaaacgttt gcttttttgc gacgcagcaa attgtcgcaa
     9361 acctggagca ggaagataac gtttcgctgg caggggattg tccgccacgc atcttgacga
     9421 aaattaaact ctcaggggat gttttcttat gtctacgcca tcagcgcgta ccggcggttc
     9481 actcgacgcc tggtttaaaa tttcacaacg tggaagcact gtccgtcagg aagtggttgc
     9541 cgggttaaca acgtttctgg cgatggtcta ctcggtcatc gtcgttccag gtatgttggg
     9601 taaagcaggc ttcccgcctg cggcagtttt cgttgcaacc tgtctggttg ccggactcgg
     9661 ttctatcgtg atgggtctgt gggctaatct gccgttggcg attggttgcg ccatctccct
     9721 gacggcgttt accgcattca gcctggtgct ggggcaacat attagcgtac ctgtcgcgct
     9781 gggtgccgtg ttcctgatgg gtgtgctgtt tacggtaatt tctgccacgg gtatccgtag
     9841 ctggattttg cgcaacttgc ctcacggtgt ggcgcacggc acggggatcg gtatcggtct
     9901 gttcctgctg ctcattgccg ctaatggtgt cggtctggtg attaaaaacc cgcttgatgg
     9961 tctgcccgtt gcgctgggtg atttcgcgac cttcccggtg attatgtcac tggtaggtct
    10021 ggcggtgatc atcggcctgg aaaaactgaa agtccctggt ggcattctgc tgaccattat
    10081 cggtatctca attgtcggtt tgatcttcga tcctaacgtc catttctccg gcgttttcgc
    10141 catgccttca ttgagcgatg aaaacggcaa ttcactgatt ggcagcctgg acattatggg
    10201 cgcgctgaat cctgtagtcc tgccaagcgt tctggcgctg gtgatgacgg cagtatttga
    10261 tgccaccgga actatccgtg ccgtcgccgg ccaggcgaac ctgctggata aagatgggca
    10321 gatcatcgac ggtgggaaag cactgaccac tgactccatg agcagcgttt tctctggcct
    10381 ggtgggtgct gctccggcag cggtatacat cgagtctgcg gcgggtacgg cggcgggcgg
    10441 taaaaccggt ttgacggcta tcaccgttgg cgtgctgttc ctgctgattc tgttcctctc
    10501 tccgctctct tacctcgttc cggggtatgc aacggctccg gcgctgatgt acgttggcct
    10561 gctgatgctg agcaacgtgg cgaaaatcga ctttgctgat tttgttgatg cgatggcggg
    10621 tctggttacg gcggtattca tcgtgctgac ctgtaacatc gtaacaggca tcatgatcgg
    10681 cttcgcgact ctggtgattg gtcgtctggt ttccggcgaa tggcgcaagt tgaacatcgg
    10741 tacggtcgtt atcgccgtgg cgctggtgac cttctatgcg ggtggctggg ctatctaatc
    10801 tttcatcgga ttctgaaaac gggtggcaat ggctgcccgt ttttattttc tccgcacatt
    10861 gtgtgagctt ttgcgatatt ctgaaaaaat gagaattcag gcataacgtc tttctcagat
    10921 cataaaaaaa catcgcaaac agggaacgca tggaaatctt cttcaccata ctgataatga
    10981 ccctcgtggt ctcgctgtcc ggggtggtca ctcgtgtcat gccctttcag atcccgcttc
    11041 cgcttatgca aatcgccatc ggtgcgctac tggcgtggcc gacgtttggt ttgcatgtgg
    11101 agtttgatcc tgaactcttt ttagtcttgt ttatcccgcc gttgctgttc gctgatggct
    11161 ggaaaacgcc gacccgtgaa tttctcgaac atggtcgaga gatttttggc ctcgcgctgg
    11221 cgctggtggt ggtcaccgtg gtcggcattg gcttccttat ttactgggtg gtgccgggca
    11281 ttccgctgat ccccgccttt gcgctggcgg cagtgctttc tccgaccgat gctgtggcgc
    11341 tctccgggat tgttggcgaa gggcgcatcc cgaaaaaaat catgggtatt ttgcagggcg
    11401 aagcgttgat gaatgacgcc tccggcctgg tgtcgttgaa gtttgccgtg gcagtggcga
    11461 tggggacgat gatcttcacc gttggcggtg cgacggtcga atttatgaaa gtagccattg
    11521 gcggtattct cgccggcttt gtggtgagct ggctgtatgg tcgctcgctg cgattcctca
    11581 gccgctgggg cggtgatgaa cccgcgacgc agatcgttct gctgttcttg ctgccattcg
    11641 cttcttatct gattgccgaa catattggcg tttccggcat cctcgctgcg gttgccgccg
    11701 ggatgaccat cacccgctcc ggtgtgatgc gccgtgcgcc gctggcaatg cgcctgcgcg
    11761 caaacagcac ctgggcgatg ctggaatttg tctttaacgg catggtgttc ctgctgttag
    11821 gtctgcagct gccgggtatt ctggagacgt cgctgatggc ggcagaaatc gaccctaacg
    11881 tcgaaatctg gatgctgttt accgatatta ttctgatata tgcggcgctg atgctggtcc
    11941 gtttcggctg gctgtggacg atgaaaaagt tcagcaaccg cttcctgaag aagaagccga
    12001 tggagtttgg ttcgtggacc acacgagaaa tcctgatcgc gtctttcgcc ggggtgcgtg
    12061 gggcgatcac tctggccggt gtgctctcta tcccgctgct cttgccggat ggtaacgtct
    12121 tcccggcgcg ctatgagctg gtgttcctgg cggctggcgt cattctcttc tcgctgtttg
    12181 tcggcgtggt gatgttgcct attctgctac aacacattga agtcgcggac cattcgcaac
    12241 aattgaaaga agaacgtatt gcgcgagcgg caacggcaga agtggcgatt gtggcgatcc
    12301 agaaaatgga ggagcgtctg gcggcggata ccgaagagaa tatcgataac cagctgctta
    12361 cggaggtcag ttctcgcgtc attggtaacc tgcgtcgtcg cgccgatgga cgtaacgacg
    12421 ttgaaagttc cgtgcaggaa gagaaccttg agcgtcgctt ccgtctggcg gcattgcgtt
    12481 ctgaacgtgc tgaactgtac cacctgcgcg ccacgcggga gatcagcaac gaaacgctgc
    12541 aaaaattact gcacgatctc gatttgcttg aagcgttgct aattgaggaa aatcagtaag
    12601 ccgaaagcga cgtaaccagg cccgaggtaa tctgaaaaag gtgcaatctt ttgcaccttt
    12661 aacaggttag ctcgggctaa cattcatgat tctaaaacaa ataagttatc tctcttgtaa
    12721 agtcaattca ttggctgttc ttcgacttaa cctcaatctg cgtgttgcat aatatctgca
    12781 gtaatcttca tagcgactgc attaatatta tcctcagttt gtgacatgcc agtatgagag
    12841 gtacaatctt cttcaaagaa tttctgtagt gttaccaggg tatcactatc aatggggata
    12901 ttttcctccc cacggagaat agaaatgccc attccctgga cgctagcctc atcaaaatta
    12961 acccgataga gacgggtatt aaacatacag gcttttgtta aaatagaaca gtt
//
LOCUS       DAZGVQ010000141        13004 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000141 DAZGVQ010000000
VERSION     DAZGVQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.142"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            271..837
                     /gene="yqaB"
                     /locus_tag="ACHLAG_003991"
     CDS             271..837
                     /gene="yqaB"
                     /locus_tag="ACHLAG_003991"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFP0657129.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            834..1261
                     /locus_tag="ACHLAG_003992"
                     /pseudo
     CDS             834..1261
                     /locus_tag="ACHLAG_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
     gene            1334..2890
                     /gene="gshA"
                     /locus_tag="ACHLAG_003993"
     CDS             1334..2890
                     /gene="gshA"
                     /locus_tag="ACHLAG_003993"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFP0657130.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            3040..3555
                     /gene="luxS"
                     /locus_tag="ACHLAG_003994"
     CDS             3040..3555
                     /gene="luxS"
                     /locus_tag="ACHLAG_003994"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFP0657131.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPEEKLQELHI"
     gene            complement(3619..5157)
                     /gene="emrB"
                     /locus_tag="ACHLAG_003995"
     CDS             complement(3619..5157)
                     /gene="emrB"
                     /locus_tag="ACHLAG_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFP0657132.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5174..6346)
                     /gene="emrA"
                     /locus_tag="ACHLAG_003996"
     CDS             complement(5174..6346)
                     /gene="emrA"
                     /locus_tag="ACHLAG_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFP0657133.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6473..7003)
                     /gene="emrR"
                     /locus_tag="ACHLAG_003997"
     CDS             complement(6473..7003)
                     /gene="emrR"
                     /locus_tag="ACHLAG_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFP0657134.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(7094..7429)
                     /gene="ygaH"
                     /locus_tag="ACHLAG_003998"
     CDS             complement(7094..7429)
                     /gene="ygaH"
                     /locus_tag="ACHLAG_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFP0657135.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7419..8156)
                     /gene="ygaZ"
                     /locus_tag="ACHLAG_003999"
     CDS             complement(7419..8156)
                     /gene="ygaZ"
                     /locus_tag="ACHLAG_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFP0657136.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8280..9464)
                     /locus_tag="ACHLAG_004000"
     CDS             complement(8280..9464)
                     /locus_tag="ACHLAG_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0657137.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(9755..10747)
                     /gene="proX"
                     /locus_tag="ACHLAG_004001"
     CDS             complement(9755..10747)
                     /gene="proX"
                     /locus_tag="ACHLAG_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFP0657138.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10805..11869)
                     /gene="proW"
                     /locus_tag="ACHLAG_004002"
     CDS             complement(10805..11869)
                     /gene="proW"
                     /locus_tag="ACHLAG_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFP0657139.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11862..>13004)
                     /gene="proV"
                     /locus_tag="ACHLAG_004003"
     CDS             complement(11862..>13004)
                     /gene="proV"
                     /locus_tag="ACHLAG_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFP0657140.1"
                     /translation="AFKYIEQGLSKEQILEKTGLSLGVKDASLAIEEGEIFVIMGLSG
                     SGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELREVRRKKIAMVFQSFALMPHMT
                     VLDNTAFGMELAGINAEERREKALDALRQVGLENYAHSYPDELSGGMRQRVGLARALA
                     INPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTIVFISHDLDEAMRIGNRIAIM
                     QNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSAKDIARRTPNGLIRKTPGFGP
                     RSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALTQQQGLDAALIDAPLAVDAQT
                     PLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRALDREGVNNG"
BASE COUNT         3220 a         3342 c         3458 g         2984 t
ORIGIN      
        1 aaccttcagc aatgaaggta tcgaagagat agcggtatta accgcaggca ccagggagga
       61 taacgttgcg tcagcaacgg cccgaagggc gaacgccagt gagtaatcct cccggatgca
      121 ccatcttctc ccaaaaattt ccttcaaaaa tatctcattt ctaaataact actttatcca
      181 tttcctgaat tttcttctcc ttcgccatca gcgacaaaat caagcaatta cgctaaagta
      241 gcgtcatatt tttcgacttg cgagaagacc atgtacgagc gttatgcagg tttaattttt
      301 gatatggatg gcacaatcct ggatacggag cctacgcacc gtaaagcgtg gcgcgaagta
      361 ttagggcact acggtcttca gtacgatgtt caggcaatga ttgcgcttaa tggatcgccc
      421 acctggcgta ttgctcaggc aattattgag ctgaatcagg ccgatctcga cccgcatgcg
      481 ttagcgcgtg aaaaaacaga agcagtaaga agtatgctgc tggatagtgt cgagccgctt
      541 cctcttgttg aagtggtgaa aagttggcat ggtcgtcgcc caatggctgt aggaacgggg
      601 agtgaaagcg ccatcgctga ggcattgctg gcgcacctgg gattacgccg ttattttgac
      661 gccgtcgtcg ctgctgatca cgtcaaacac cataaacccg cgccagacac atttttgttg
      721 tgcgcgcagc gtatgggcgt gcaaccgacg cagtgtgtgg tctttgaaga tgccgatttc
      781 ggtattcagg cggcccgtgc agcaggcatg gacgccgtgg atgttcgctt gctgtgagtg
      841 aagcgttatc gcttttctcg ttgtttgcca gtagttttct cagcgctaca ctattacccg
      901 gcaactccga agtcgtgctg gtggcaatgt tgctttccgg gatcagttat ccctgggttt
      961 tagttttaac agcaacaatg ggtaatagcc ttggagggtt aactaacgtt atccttgggc
     1021 gtttctttcc attgcgtaaa acatcgcgct ggcaagagaa agctactggc tggctgaaac
     1081 gctatggtgc agtcacgcta ttattaagct ggatgcccgt ggttggcgat ttactgtgtc
     1141 tgttagcggg atggatgcgc atctcgtggg gaccggtaat ctttttttgt gccttggtaa
     1201 agcgttacgc tatgttgcag ttgcagcagc taccgttcag ggcatgatgt ggtggcacta
     1261 attgtaggcc tgcacatatg gtcaccatta cagttatgct aattaaaacg attttgacag
     1321 gcgggaggtc aatttgatcc cggacgtatc acaggcgctg gcctggctgg aaaaacatcc
     1381 tcaggcgtta aaggggatac agcgtgggct ggagcgcgaa actttgcgtg ttaatgctga
     1441 tggcacactg gcaacaacag gtcatcctga agcattaggt tccgcactga cgcacaaatg
     1501 gattactacc gattttgcgg aagcattgct ggaattcatt acaccagtgg atggtgatat
     1561 tgaacatatg ttgaccttta tgcgcgatct gcatcgttat acggcgcgca atatgggcga
     1621 tgagcggatg tggccgttaa gtatgccatg ctacatcgca gaaggtcagg acatcgaact
     1681 ggcacagtac ggcacttcta acaccggacg ctttaaaacg ctgtatcgtg aagggctgaa
     1741 aaatcgctac ggcgcgctga tgcaaaccat ttccggcgtg cactacaatt tctctttgcc
     1801 aatggcattc tggcaagcga agtgcggtga tatctcgggc gctgatgcca aagagaaaat
     1861 ttctgcgggc tatttccgcg ttatccgcaa ttactatcgt ttcggttggg tcattcctta
     1921 tctgtttggt gcatctccgg cgatttgttc ttctttcctg caaggaaaac caacgtcgct
     1981 gccgtttgag aaaaccgagt gcggtatgta ttacctgccg tatgcgacct ctcttcgttt
     2041 gagcgatctc ggctatacca ataaatcgca aagcaatctt ggtattacct tcaacgatct
     2101 ttacgagtac gtagcgggcc ttaaacaggc aatcaaaacg ccatcggaag agtacgcgaa
     2161 gattggtatt gagaaagacg gtaagaggct gcaaatcaat agcaacgtgc tgcagattga
     2221 aaacgaactg tatgcgccga ttcgtccaaa acgcgttacc cgcagcggcg agtcaccttc
     2281 tgatgcgctg ttacgtggcg gcattgaata tattgaagtg cgttcgctgg acatcaaccc
     2341 gttctcgccg attggtgtag atgaacagca ggtgcgattc ctcgacctgt ttatggtctg
     2401 gtgtgcgctg gctgatgcac cggaaatgag cagtagcgaa cttgcctgta cgcgcgttaa
     2461 ctggaaccgg gtgatccttg aaggtcgcaa accgggcctg acgctgggta tcggctgcga
     2521 aaccgcgcag ttcccgttac tgcaggtggg taaagatctg ttccgcgatc tgaaacgcgt
     2581 cgcgcaaacg ctggatagca ttaacggcgg cgaagcgtat cagaaagtgt gtgatgaact
     2641 ggttgcctgc ttcgataatc ccgatctgac tttctctgcc cgtatcttaa ggtctatgat
     2701 tgatactggt attggcggaa caggcaaagc gtttgctgaa gcgtaccgta atctgctgcg
     2761 tgaagagccg ctggaaattc tgcgcgaaga ggattttgta gccgagcgcg aggcatctga
     2821 acgccgtcag caggaaatgg aagccgcaga taccgaaccg tttgcggtgt ggctggaaaa
     2881 acacgcctga cagaaaagaa aaaggccact cgtgagtggc caaaatttca tctctgaatt
     2941 cagggatgat gataacaaat gcgcgtcttt catatactca gactcgcctg ggaagaaaga
     3001 gttcagaaaa tttttaaaaa aattaccgga ggtggctaaa tgccgttgtt agatagcttc
     3061 acagtcgatc atacccggat ggaagcgcct gcagttcggg tggcgaaaac aatgaacacc
     3121 ccgcatggcg acgcaatcac cgtgttcgat ctgcgcttct gcgtgccgaa caaagaagtg
     3181 atgccagaaa gagggatcca taccctggag cacctgtttg ctggttttat gcgtaaccat
     3241 cttaacggta atggtgtaga gattatcgat atctcgccaa tgggctgccg caccggtttt
     3301 tatatgagtc tgattggtac gccagatgag cagcgtgttg ctgatgcctg gaaagcggca
     3361 atggaagacg tgctgaaagt gcaggatcag aatcagatcc cggaactgaa cgtctaccag
     3421 tgtggcactt accagatgca ctcgttgcag gaagcgcagg atattgcgcg tagcattctg
     3481 gaacgtgacg tgcgcatcaa cagcaacgaa gaactggcgc tgccggaaga gaagttgcag
     3541 gaactgcaca tttagtcagt aaactatctt cacaattaat tgaaaaaagc cagttcaaat
     3601 gaactggctt agttgtactt agtgcgcacc gcctccgccg ccacctgcgc caaatggcgg
     3661 tttagcaaac cacaccagcc ccagcaggac gaggaatatc ccggctgaca tccagaagat
     3721 ctcattggcg gaaataatca gcccctggtt ggtgatctgc tgggcaatcc agccggaggc
     3781 ctgctgttgc gtcatcccaa gcccttccag ttgactgtac atcgcctggg cgtttgggtt
     3841 aaacgggttt accgactcgg ttaactgtgc gtggtgcatc gactcgcggt tggtccacat
     3901 ggtggtggtg atggacgtgc cgatagaccc cgccagcgta cgcgtaaagt tggagaggct
     3961 cgatgccgcc gccagtcgtt ccggtggcaa accagacagc gtaatggtgg tcagcggcat
     4021 aaagaagcag gccaccgcaa acccctggat aaactgcggc caggccgacg cgccaaaatc
     4081 catacctggt tcaaaggtat aggcacgcca gtagaagcag acggcataca taataaagct
     4141 gaaggttacc agccgccgca tatccagttt atgcgcgaag cggccgataa tcggcgacag
     4201 gatcaccgga ataatcccta ccggcgcaga ggccaaacct gcccaggtcg ccgtgtaacc
     4261 gtagacctcc tgcaacaact gcggcagcag aacaatagcg ccgaagtaga gcatatacgc
     4321 gaggctgata cacaagcagc cgatggtgaa gttgcgcgac ttaaacaacg acagatcgac
     4381 tatcgggtta tcgtcggtca gctcccagac aatcaggaag cagatagcca ccaccgccac
     4441 cacggtaagg atgataattt cctgtgatga aaaccagtcc agctctttac cgcggtcgag
     4501 cataatctgc aggctgccga taccaataac cagcagtgcc agccccacgg catcaatccg
     4561 ccgccgttcg gtgcgggttt cacgtccgcg cagagtttgc agtgtcatca acaccaccgc
     4621 cacgccaatc ggcacgttga tgaagaatat ccagccccag tggtaattat cgctgatata
     4681 accgccgagg atcgggccgc aaattggcgc gacaatcacc gtcatcgacc acaacgccag
     4741 cgcgatcgag cgtttggctg gcgggtagtt attcagcaat agactttgcg aaagcgggat
     4801 caacggcccg gcgacaatcc cctgaatcac gcggaagaag atcagcatat tcaggctgct
     4861 ggagacacca cacgcccacg acgcaatagc aaaggcgatg gtggaccaaa ggaacagttt
     4921 cacttccccg acgcgctttg ccagccagcc ggtaagcggg atcgagatgg cattcgccac
     4981 cccgaaagaa gtgattaccc acgttccctg gctgagcgat gagcccagat tcccggcgat
     5041 agtggggatc gccacgttag caatggtgga gtccagcacc tgcatgaatg tcgccagtga
     5101 cagcgcaatc gtcataatga ccagttgcgc gccttccagc ggtttttgct gttgcatcac
     5161 acgcacctct ggattagcca gcgttagctt ttacgatatc gtcgatcagt ttattgacag
     5221 gtgccaggct gatttcacgc gcggtgctta ccgctaccgg agtggaacgt actttatttg
     5281 ccagtacctg accgtcacgg ttagtggtat tgacgctcac cagcgtggac aaaccgatac
     5341 gcagcggata ttgctccagc tgtttctggt ccagttcgat acgcacaggc agacgctgaa
     5401 cgactttgat ccagttaccg gtcgcatttt gcgctggaag cagtgagaac gcgctacctg
     5461 tgcccatatc cagaccaacc actttaccgg tgtatttcac atcatcgccg taaatatccg
     5521 tggtgatagt gaccggctga ccgatacgca tattggcaat ctgcgtctct ttaaagttgg
     5581 catccaccca catattggtg gctggaacga ccgccatcag cggcgtcgtt gggctaattt
     5641 gcgccccagg ctgtaccgcg cggcgggaga cataaccggt catcggactg acaatacgag
     5701 tacgctccag cgccagccag gcgttacgta cttcggtggc agcctgttgc acggctggct
     5761 gatcttccag tttagtcccc agaatcatcg cctgattggc attgtattgt tgaatcgcga
     5821 cgtccagttg cgcctgggca ctggtgacgg cgtcgcgggc gtgttgcagc tcttcgcgac
     5881 caatcaggtt ggcattgccc agcggcacac ggcggttgta gtcgctttgt gctttcgcga
     5941 gggcgatttt ctgcacctca atattcgcct gcaactgctt gctgttaatc atcagctggt
     6001 gggtttggcg aacgctggaa gccagtgcag ttttggcttt ttcaaacgcc tggcgagcat
     6061 ctgtcgggtc gagagtgacc agcacgtcgc cttcttttac aaaatcggtg ttatcggccc
     6121 agactttcgt cacgctgcca gacacctgag acataatttg aatttgattc cctgccacgt
     6181 atgcgtcatc ggtttcttcg aaatgacgca gtaccaaaaa ccaataaatc cctatcgcta
     6241 cggcaataat tataaagagc aaggtgagaa ggaggagcag acgcttacgt ttgccgctct
     6301 tctttaccgg ttgctgcggg gtttgagtct ccgcatttgc gctcatattg ttctccacga
     6361 tcttcttaac tcatcggctg agccgacctg cttgccaaaa aggccagcat gttgcgatgc
     6421 tggccggtca ttttttcttt tataaatctg gatttttgag cgagatgacg cgttagctca
     6481 tcgcttcgag aaccacaccg tcttgttcca tctggtcgag acgggagagc aatttgcggg
     6541 tgatttgctc gagctgatct ttttctgttg tgctgagcgc ggaccagagt tgatgcaggc
     6601 agttatgctg cggtggtaaa acctcgcgca aaaactcgtg acctttttcc gttaattgca
     6661 gatgcaggca gcggcgatcg ttatcgcttt cacgacgttc gatccaaccg cgtttttcca
     6721 gttcatcggc aatacgcgtc gcgttggtac gggatgatcc aagagcacaa cttaattcag
     6781 aaggctgaat actgtggttt tcctgagact ccagcgtaat caacgccata aacaacgtct
     6841 cgttaatccc ctgagccttc agcattttat tgcggttctc cagcagcttg ctttgcatgt
     6901 gcatgcaaag acgagtcaga aggatctcct gataaggaaa atcttcgtgg cggctggcgc
     6961 gaaattttag catttgttca atgggcgtaa acgaactatc catttgggta tgacctcatt
     7021 aatttcatcc gatatagtaa cgacagtgac aaataaagta aatgtattgt tttagaaaaa
     7081 tgattcttgt gggttatata atcgccatca ctttccaggc gagcccatag gccagcgcac
     7141 taagcagtgt tgggatgata atgctgcgtg ttttatagaa actggcaccc agtaccgcga
     7201 agccgaccag cgtgggcacg aaacggcgtg tatcgtgcat cacttctggt gcggtagaga
     7261 caaccagcag agcgcatatc gaggcgatgc caatggtgtc gagcaaaata cctaccgcgc
     7321 cacgtttggt tgggcgggca ttacccacac gcaggcgcag cggcaaatag cggaagcaat
     7381 aattcgccac gccaaccagt aacccaagca gcagaacctc atagctcatc gggcgctcct
     7441 tgccagaatg cctggattaa cgcagtgagg cagccacaga caatgcctgc cagaatggcg
     7501 acgggaatag aaaatagcgt tacgcctgca agggcaccaa ctaacgctgc ggtaacgcaa
     7561 agagattgtt tgcgctggaa agaggcgagc aggaaactca taaagagtgc cggaagcata
     7621 aaacctaatg cagcttcaac ggcgggataa ccttgcagca agccgctgcc ggagaatgcc
     7681 cctattaccg taccaaatac ccacgatgac catgaactga aggcaatgcc gatcatccag
     7741 ttctcgctcc agcggcgatt attgcgtacc agttttgcgg ttgcggcggc aaaaacctca
     7801 tccgtcaggc caaatgccca cagggcggtt ttcgattttt gcagacgctg aataatacgg
     7861 ctacgcagtg acgggccata caacacatgg cgaacatcca ttgccatgac ggtcagtgca
     7921 gcaacccaca aactactccc ggctgccagc atcgcggtaa tgacgaactg gctcgcgcct
     7981 gcataaatga tgcaggagaa aaaaacgctt tcgagaggag agaatcccag acgggtcgca
     8041 ttcagaccga acgcaaaggc caccggaata taactaataa caatcggtaa actgtctttg
     8101 catccttcca tgaaggtcgc cgaaccagga gcaggctgtg gagtagggct ttccatagag
     8161 tgtacgctta acgattgtta atattataaa tagactgaat gaatatctta accttatcag
     8221 actgatgggc ttcttaacac ccttataagt gtaaagccac gaaaacggtt gctgattgct
     8281 taatttgcgg cttcctgacg atgaaaacct cgccaccaga ccagtaagtt aaccagggca
     8341 atcgtcgcgc cagccagaca aacgccagcc caaccgccat gttgccaggc tgaggctgaa
     8401 attagcgaac cggcggcacc gccaataaag tagctggtca tgtaacctgc ggtcaggcga
     8461 ttacgcgcat cagggtgtat tcgataaatt accgtctggt tagtgatatg cacgccctgt
     8521 acggtgagat ccagcaccag gatgccgata atcagcgcca gtacggaagt gtgaccaaac
     8581 cagatcgcca gccatgaaag taatagcagc agcagaccga aagttgtggt gtggtgcgat
     8641 ttacccttat cggcaaaacc gcccgccgga cgagcgccca acgctccggc agctcccgca
     8701 agtccaaaca gaccaatgac accatcgctg tagttaaaag gtggagcggc aagcaaaaag
     8761 gccattgagg tccagagaat gctgaagttg gcaaaggtca ggcagcccag caatgcgcgg
     8821 gtgcgcagga tcttatcgct gataaacatg ctgaaaacgg aacctaacaa ctgtgggtag
     8881 ttgaggtggg tttctgattt catttgtggc agaccacgcc ataatgccag cgccatcagt
     8941 gccattaaca ccgaggcaac ccaaaagacg gtgcgccagc cgccgagatt cgccagcaat
     9001 ccggcaacgg tccgtgcgag taagatcccc aacagcagac cgctcataat agtgccaacc
     9061 actttgccgc gtttgtccgg tgaagccagc gtcgctgcca gcggaaccag aatttgtgcc
     9121 acgacagaga ataaaccggt taatgccgta ccgaggatca tcatcgccag tgactgactg
     9181 ctggcagtga ttaacatacc gcccgctgcc agtaaggtca tcgagacaat caggcggcgg
     9241 cgttcaaaca tatcaccgag gggaacaaga aacagcagac ctgctgcata gcccaattgc
     9301 gcggcggtaa caataaagcc tgccgaactg gcggaaaggg aaaagttacg cgcgatggtg
     9361 tcgagcaatg gctgggcgta atagttactg gctaccgcca gaccggtggc gatagacatc
     9421 agcacgatca gcgccgggct aagctcatga ttaggtttag tcattatttt tgttcatcag
     9481 gtgatgatta agctggaaat caataatagc ggatgttgat gaatatggga tgaaagatag
     9541 attgttgaaa tgtgcgatgt ggtttgtagg catgataaga cgcgccagcg tcgcatcagg
     9601 cattgtgcac agaacggcgg atgcggcgaa aacgccttat ccgccatacg aatgagtgta
     9661 ttttgtaggc atgataagac gcgtcagcgt cgcatcaggc attgtgtaca gagcggcgga
     9721 tgaggcaaaa acgccttatc cgcctgaata aaaattactt ctgcgctgcc agcgcctcat
     9781 tcacccagcc atcgaactgc tgctggtggg ctttgatcca gccatcaacg tggccctgaa
     9841 tatcgccttc tgaggctttg ccgtcatgca taatggcgtt ctgggcgtta atatcggcca
     9901 ctggcaactg cataatggca aacagtttgg ctgccgccgg atttttctcg gtccaggctt
     9961 tgttggcaac gatatgcatg gtgctgaccg ggaagccgta attcgcgcca ttcggcagtt
    10021 tggtatcggc atttttatcg cccggcagtg cggagaacgg cacctgcaac cagaccacat
    10081 ctttccctgg cttcagctca ttactcaccc agtacggcgt ccaggtgtaa taaaacaccg
    10141 gtttgccctc tttgtagcga ctgatggtgt cggccatcat cgctgcgtag ttcccctgat
    10201 tatgcgtcac ggtgttggtc agttcatacg cggcaagctg gtggttgatg gcaccttcgc
    10261 agccccagcc agggttacaa ccggttaaat ccgctttacc gtcgccgttg gtatcgaaca
    10321 gtttggcgat cttcggatct ttcagttgtg cgatgttggt gattttatac tggtcggcgg
    10381 ttttcttatc gatcaggtaa ccctgtgccg cgccgttaac aaatacccct tcacgataaa
    10441 atttcttatc gccaccggca gcttcgtaca tgttgtcatg cagtggcgtc cagttcacgg
    10501 cggtgaaggt tgcatcgccg gaagcaagcg aggtgtagcc aacgttgtaa tctacttcgc
    10561 tgggtttgtt gacggtatag cctaatttct ccagcgcacg gctgaccagc agcgtctgga
    10621 aggtttcttc ggtgatggtg ctttgaactg gatttacagt aatacctttg cccggcagat
    10681 cggcagcaaa agtttgtgta gagataagcg tggcaaacgc tgtcgcaaaa agtacgctat
    10741 gtcgcattgt tattcctttt tttggcaggg tgataatgcc cggcgtcacc gggcaagtgc
    10801 agagttactt aatgaatggg cgggtcagca gaccaacagg gccagtggtg taccagcgac
    10861 ggttgccgcg actgcgtgag tcgcgcccaa cggcctgcgt caggcgatcg aggataatgg
    10921 cgaggatcac aatcccgacg ccgccaacgg tggcaagccc catatccaga cgaccgatac
    10981 cgcgaagtac catctgaccc agcccgccga cggcaatcat cgaggcaatg accaccatag
    11041 aaagggccag catcagcgtc tggttaacgc ccgccataat ggtcggcatc gccagcggta
    11101 actgaacttt gaacagcatc tggcgcgggc tggcaccgaa tgagcgcgag gcttcaatca
    11161 gatccgccgg aacctggtta atccccagaa tggtcagacg gataatcggc ggcagagcaa
    11221 agatgatcgt caccaccacg cccggcacgt taccgatacc aaatagcatg acgattggca
    11281 ccagataaac aaacgctggc gtggtctgca tggcatcaag cagtggacga ataattttcg
    11341 ccgctcgcgg acttctcgcc agccagatcc ccagcggcaa accaatgacg atacagaaca
    11401 gcagggcggt taacaccagc gccagggtaa ccatggcctg cgaccaggca ccgattgcgc
    11461 cgatggcaat cagcgaaacc agcgtcgcca cgcccattcc gaccccggaa atctgccagg
    11521 cgatgagagc gaaaacgata atcgccaccg gtgcgggcat acccagcagc aattgctgga
    11581 aaccgttgag aatgtaatca accggaacgc gcacgccctg gaagacggga cggaaatggg
    11641 taacaaccca gtcgatccct tcagtgaccc aactgtcgag cgggatcagc gttttatgga
    11701 acggatcgag aatattaaaa tgctcgacgt ttggcgcagg cgtactggtc agccagtcag
    11761 caccaccacc gtcagtcggt gcagtcgccg gtgtacccca ggcgtctgcg gattgtacgg
    11821 cactgtccgc cgctggcgtg gtatcccacg gattattttg atcagccatt atttaccccc
    11881 tcacgatcta aagcgcgcag cagcattcct ttcgaaatga tgccgacata ctgttggtcc
    11941 tcgtcgacca cgggcaccgc acagggtgcc tgtccgacat gagagagcaa ctcgctaaga
    12001 ggcgtttgtg catcgactgc taacggcgca tcaatcagcg ccgcatcaag accttgctgc
    12061 tgcgttaacg cggttttaag cgaatcgatg gaaactgcgc cgacaaactt attaccgcgt
    12121 tcgataacgt agccatattc gcggtcttca tcctgcaata atttcagcgc cgaacgtggg
    12181 ccgaagccag gggttttacg aattaagccg ttcggtgtcc ggcgagcaat atctttcgca
    12241 ctgaatacct gactaatatc gacgccacgg aagaaggtac ggacataatc attcgccgga
    12301 ttattgagaa tttcatccgg tgtgccgacc tgtaccactt caccattttg cataatggca
    12361 attcggttgc caatacgcat ggcttcatca agatcgtggg aaataaagac aatggtgcgc
    12421 tgatgtttcg cctgtaattt taccagctca tcctgcatct cggtgcgaat taatggatcg
    12481 agcgccgaga aggcttcatc cattaataaa atatccggat taatcgctaa tgcacgggct
    12541 aatcccacac gttgacgcat cccgccagag agttcatccg ggtagctgtg ggcataattt
    12601 tccagcccga cctgacgcag tgcatcaagg gctttttccc ggcgttcttc ggcattaatt
    12661 ccggccaatt ccataccgaa cgcagtattg tccagcacgg tcatatgcgg cattaaggca
    12721 aaggactgga agaccatcgc aatctttttt ctgcgcacct cacggagttc ggcgtcggat
    12781 attttggcaa tatccacacc atcaatcagc acttgcccgc gggtgggttc aatcaggcga
    12841 ttgagaaggc gtaccattgt ggatttaccc gagccggata atcccatgat gacaaatatc
    12901 tcgccttctt caatggccag actggcgtct tttacgccaa gcgatagccc agttttttcc
    12961 agaatttgtt cttttgaaag tccttgttcg atatatttga acgc
//
LOCUS       DAZGVQ010000142        13001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000142 DAZGVQ010000000
VERSION     DAZGVQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.143"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            228..809
                     /gene="mdaB"
                     /locus_tag="ACHLAG_004004"
     CDS             228..809
                     /gene="mdaB"
                     /locus_tag="ACHLAG_004004"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFP0657141.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            840..1154
                     /locus_tag="ACHLAG_004005"
     CDS             840..1154
                     /locus_tag="ACHLAG_004005"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFP0657142.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1202..3094)
                     /gene="parE"
                     /locus_tag="ACHLAG_004006"
     CDS             complement(1202..3094)
                     /gene="parE"
                     /locus_tag="ACHLAG_004006"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFP0657143.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3123..3704)
                     /gene="yqiA"
                     /locus_tag="ACHLAG_004007"
     CDS             complement(3123..3704)
                     /gene="yqiA"
                     /locus_tag="ACHLAG_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFP0657144.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3704..4531)
                     /gene="cpdA"
                     /locus_tag="ACHLAG_004008"
     CDS             complement(3704..4531)
                     /gene="cpdA"
                     /locus_tag="ACHLAG_004008"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFP0657145.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPYQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4556..4978)
                     /locus_tag="ACHLAG_004009"
     CDS             complement(4556..4978)
                     /locus_tag="ACHLAG_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFP0657146.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(4979..5608)
                     /gene="nudF"
                     /locus_tag="ACHLAG_004010"
     CDS             complement(4979..5608)
                     /gene="nudF"
                     /locus_tag="ACHLAG_004010"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFP0657147.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5813..7294
                     /gene="tolC"
                     /locus_tag="ACHLAG_004011"
     CDS             5813..7294
                     /gene="tolC"
                     /locus_tag="ACHLAG_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFP0657148.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7442..8113
                     /locus_tag="ACHLAG_004012"
     CDS             7442..8113
                     /locus_tag="ACHLAG_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFP0657149.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8119..9279
                     /locus_tag="ACHLAG_004013"
     CDS             8119..9279
                     /locus_tag="ACHLAG_004013"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP0657150.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(9317..10105)
                     /gene="ygiD"
                     /locus_tag="ACHLAG_004014"
     CDS             complement(9317..10105)
                     /gene="ygiD"
                     /locus_tag="ACHLAG_004014"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFP0657151.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPMLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10248..11021
                     /gene="zupT"
                     /locus_tag="ACHLAG_004015"
     CDS             10248..11021
                     /gene="zupT"
                     /locus_tag="ACHLAG_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFP0657152.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11079..11249)
                     /gene="yqiD"
                     /locus_tag="ACHLAG_004016"
     CDS             complement(11079..11249)
                     /gene="yqiD"
                     /locus_tag="ACHLAG_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFP0657153.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11510..12163)
                     /gene="ribB"
                     /locus_tag="ACHLAG_004017"
     CDS             complement(11510..12163)
                     /gene="ribB"
                     /locus_tag="ACHLAG_004017"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFP0657154.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12267..12415)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12537..12827
                     /gene="ubiK"
                     /locus_tag="ACHLAG_004018"
     CDS             12537..12827
                     /gene="ubiK"
                     /locus_tag="ACHLAG_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFP0657155.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3299 a         3463 c         3165 g         3074 t
ORIGIN      
        1 cccaaaaaaa accgaaccaa acgtgtaaat tattccggtg cgagtaagaa ttatgaggaa
       61 tggctatcag tattgtcatt ttcagaaaat atttatcctg catcggtgag tcagagcaag
      121 atcagacttt tgctaaattc gcaaaagact ttgcacattt tgctaatttc accgtaccgc
      181 tctgtgacgt actatagtcg gcaaacgtct caccttgagg ttaaaaaatg agcaacatcc
      241 tgattatcaa cggcgcgaaa aaattcgccc actccaatgg tcaactgaac gacaccctga
      301 ccgaagtcgc ggatggcaca ctgcgcgacc tcgggcatga tgtccgcatc gttcgcgccg
      361 acagcgacta cgatgtcaaa gcggaagtac aaaactttct ctgggctgat gtggtgatct
      421 ggcagatgcc aggctggtgg atgggcgcgc cgtggacagt gaaaaaatat attgatgatg
      481 tattcaccga aggtcatgga acgctgtatg ccagcgatgg ccgtacccgc aaagatccgt
      541 caaaaaaata cggttccggc ggcctggtac agggcaaaaa atatatgctt tctctgacct
      601 ggaacgcacc aatggaagcc ttcaccgaaa aagatcaatt cttccacggc gttggcgtcg
      661 acggtgtgta tctgccgttc cataaagcaa accaattcct tggcatggaa ccgctgccga
      721 catttatcgc taatgacgtg ataaaaatgc ctgatgttcc ccgctatact gaagaatatc
      781 gcaagcatct tgtggaaatt tttggttaac tagagctcag gctttagaag gagttaacca
      841 tgcttaccgt aatcgcagaa atccgtactc gtcctggtca acatcaccgt caggcggtat
      901 tggatcagtt tgctaaaatc gttccaaccg tactgaaaga agaaggttgc cacggctatg
      961 cgccaatggt ggattgcgct gctggtgtga gtttccagtc tatggcaccg gattctatcg
     1021 tgatgattga gcagtgggaa agcatcgcgc atcttgaagc gcatctgcaa accccgcaca
     1081 tgaaggcgta cagcgaagcc gtaaaaggcg acgtgctgga gatgaatatc cgcatcctgc
     1141 aacctgggat ttaaacctgc cttgtttgcc cggccatcct gaccgggcaa tgttctttcc
     1201 tttaaacctc aatctccgcc atgtcgcctt tctcttgcaa ccagttgcgg cgatcttccg
     1261 agcgtttctt cgccagcagc atatccatca tcgcgtcagt acgctgatcg tcttcatcat
     1321 cgatagtcaa ctgcaccaga cggcgagtgt tcggatcaag cgtggtttca cgcaattgca
     1381 tcgggttcat ttcccccaga cctttaaaac gctggacgtt cggcttgcct ttcttgcgtt
     1441 ttaattgctc aagtacgccc tctttctctt cttccgtcag cgcgtaataa acctctttcc
     1501 cgagatcaat acggtagagc ggtggcagtg cgacgtaaac gtgaccgtgt ttcaccaacg
     1561 cgcggaagtg ttttacgaac aaagcgcaga gcagcgtggc aatgtgcaga ccatcagagt
     1621 ccgcatccgc gaggatacag attttgccat aacgaagctg gctcagatcg tcgctgtcag
     1681 gatcgatacc gatcgctacc gaaatatcat gcacttcctg cgaggccagc acttcgtcgg
     1741 aagaaacttc ccaggtgtta aggatcttac ctttcagtgg catgatcgcc tgatattcgc
     1801 gatcgcgcgc ctgcttggca gatccgcctg cggagtcacc ttccacaagg aacagttcgg
     1861 tacggttaag atcctgcgcg gtacagtcag ccagtttgcc aggcagtgct gggccgctgg
     1921 tcagcttttt gcgcaccact tttttagccg cacgcatacg gcgctgggcg ctggaaatcg
     1981 ccatctccgc cagcagctcc gccgcctgaa cgttctggtt cagccacagg ataaaggcat
     2041 ctttcaccac gcctgaaacg aatgccgcgc actgacgcga agagagacgc tctttggtct
     2101 gcccggcaaa ctgcggatcc tgcattttta ctgacagcac ataggcgcag cgatcccaga
     2161 tatcttccgc cgacaacttt acaccgcgcg gcagaatgtt gcggtattca cagaactcac
     2221 gcatcgcgtc caacaggccc tgacgcagac cgttaacgtg cgtaccgccc tgcatcgttg
     2281 ggataaggtt gacgtagctt tcggtcagca gttcaccgcc ttccggcagc cacagtagcg
     2341 cccagtccac agcttcagta tcaccagcga aattaccgat aaacggtttt tctggcagcg
     2401 tcggtaaacc gtttaccgct tccgccaggt aatcattcag accgtcctgg tagcaccagc
     2461 gctgttcggt gttgttgatc tcatctttaa aggtaatctc aacgccaggg cacagtactg
     2521 ctttggcctt cagcacatgc gtcaggcgtg aaacagaaaa acgcgggctg tcaaagaagg
     2581 tttcatccgg ccagaagtgc acgctggtac cagtattgcg tttaccgcaa gtgccgacaa
     2641 cctgtaaatc ctgcaccttt tcgccatttt caaaggcgat gttataaacc tgaccatcgc
     2701 ggcgcacgtt aacttctacg cgcttcgaca gggcgttaac caccgaaatc cccacgccat
     2761 gcaggccgcc agagaactgg taatttttgt tagagaattt accgcctgca tgcagacggc
     2821 aaagaatcag ttcaaccgcc ggtacaccct cttccgggtg aatatccacc ggcatcccgc
     2881 gcccatcgtc aataacttct aacgactggt cagcatgtaa gattacatcc acgcgttttg
     2941 cgtgacccgc cagtgcttca tcgacactgt tatcaataac ttcttgcccc aaatggttag
     3001 ggcgagtggt atcggtatac atccccggac ggcggcgaac cggctcaagc ccggtgagta
     3061 cctcaatggc atcagcgtta taagtttgcg tcatggttta agttagcaat tcgagttgat
     3121 cgtcagagat ggtgcagacc aagaaaatcg acgatcgggt tgaaataatc ttcgaagccc
     3181 gtgaatgcgt ggttgccgcc ttctatgaca gtctggcggc aggaagcgta gtacgccacc
     3241 gcctggcggt aatccagcac ttcatctccc gtctgttgca gcagccagat caaatccggc
     3301 gcttccagcg ggtcaatctg catgactttg agatcgtaaa tatggcgtga ctctagcaca
     3361 tattgctgcc cggtgtaggg gttctcgttc tgaccgagat agtccgtcag cagttcaaac
     3421 gggcgcaccg ccgggtttac caccactgcg ggcagcataa aacattgtga caaccaggtg
     3481 gcgtaatatc cccccagtga cgaaccgaca atacccagcg aatcgccgcc atgttccagg
     3541 acaatggatt ccagcagctc tgccgcgtcg gaaggatacg gcggcaactg cggaatgatc
     3601 atctcaacgt cagggtgatg ttccgccagc cagtttttta acaagctcgc ttttgcagag
     3661 cgcggcgagc tgttgaaacc gtgtaaataa agaagcgtag acatcagtag ccttctgaag
     3721 cggtatcagg ttggaaacgt gtgtccgcca ggcgatgcac ctcggtggtc agcgtgccat
     3781 cagcatgtaa ctcgagagta cgccagccgg gcgcgatagt atccagcgta aagttggaac
     3841 agtgcggctt aaactgcaca caggtcgacg gcgttgccag caggcggcga ccgttccagt
     3901 cgagatccag ctcctgatga atatgaccgc acagcaagta tttgacgtgc ggaaacttcg
     3961 ccagcacggt atccagttcg cccgcgttac gcagactgtg ttgatcgagc caactacaac
     4021 ccgcaggtag cggatgatga tgcagcagca gcaacgtatg gcgttctggc gcatcggcca
     4081 gtttacgttc cagccactca agctgaaact cgctcagctc accgtgcggc acgccaaaca
     4141 cctggctatc cagcaacagg atttgccatt gctcaccaat aaacacgcgc ttcgccgggg
     4201 agatacccgc atcctgtaac gcgctgtaca tcgcgggctg gaaatcgtgg ttgcccggca
     4261 gccagacgca gggcgcacga aaacttgcga tgccttcagc gaaatgctga taggccgcag
     4321 aggattgatc ctgcgctaaa tcacctgtcg cgacaatcag gtcgaattcg tgctgatatg
     4381 gacgaatcgc ctccagcacc gcctggtagc tttcccaggt gtttacccct aacagggctt
     4441 cgtgcttttg tgcaaacagg tgagtatcgg taatttgtaa aatcctgact ctggcctcac
     4501 cagccagagg aagggttaac aggctttcca aatggtgtcc ttaggtttca cgacgctagt
     4561 aaaccggaat cgccatcgct ccatgtgcta aacagtatcg caaccagtcc gctaaaaact
     4621 ggttaatttg atgcttttcg tcgcgttgat gcaatttttt attaggataa tcataccgcg
     4681 ctttgaagcg aaaaatctgc tggcttgaac acacttcagc caccatcgcg tcatgataca
     4741 gacgcaccgt cattgacgga aggctccagt aactgatcgc gggcgcagtc tgttctattg
     4801 tcaccagggt agtgtatcgg gtcgattcca caatcgtcag ccgatattgt gcgtttgcca
     4861 cctgatagct tacagtttcg ccgggtgcgt cattgcgcgg taacaaacgg cgcaattgtg
     4921 aaaagttcat ctcgcacagg cgcatcattt caggaaagtc aggtgtataa cgcttcattt
     4981 atgcccactc attttttaac gcttgatgat gcagctgcag ccattgcaaa gcgatgaccg
     5041 acgctgcgtt gtcgattttc ccctcttcta cccactggta tgcctgttcc cggcttacca
     5101 catgaacgcg aatatcttcg ttttcatcag ccagaccatg aataccgctt gcggtcgtgg
     5161 cgtccacttc gcccaccata attgacgaac gctcactggt gccccccggg cttgccagga
     5221 aacttaacac cggtttggtc cgtttgacta tcagtcccgc ctcttcaatc gcttcgcgac
     5281 gggcaacatc ttccacactt tcaccctctt ctatcatccc ggcaaccatc tccagtagcc
     5341 agggggtttc actggtgtcg tatgcggcaa tccgaatctg ctcaatcagc acaacttcat
     5401 cacgcactgg gtcaaagggt agcaagactg cggcgtgacc gcgctcaaaa atttccctcc
     5461 gtacctcatg actcatttgc ccgttgaata aacgatgacg aaatctataa agatctaatg
     5521 aaaaaaagcc gcgataaagt gtttctcgtg caataatttc tacatcgttt ttgccaaatg
     5581 taacgggcag gttgtctggc ttaagcattg ttaatgtcct ggcactaata gtgaattaaa
     5641 tgtgaatttc agcgacgttt gactgccgtt tgagcagtca tgtgttaaat tgaggcacat
     5701 taacgcccta tggcacgtaa cgccaacctt ttgcggtagc ggcttctgct agaatccgca
     5761 ataattttac agtttgatcg cgctaaatac tgcttcacca caaggaatgc aaatgaagaa
     5821 attgctcccc attcttatcg gcctgagcct ttctgggttc agttcgttga gccaggccga
     5881 gaacctgatg caagtttatc agcaagcacg ccttagtaac ccggaattgc gtaagtctgc
     5941 cgccgatcgt gatgctgcct ttgaaaaaat taatgaagcg cgcagtccat tactgccaca
     6001 gctaggttta ggtgcagatt acacctatag caacggctac cgcgacgcga acggcatcaa
     6061 ctctaacgcg accagtgcgt ccctgcagtt aactcaatcc atttttgata tgtcgaaatg
     6121 gcgtgcgtta acgctgcagg aaaaagcagc agggattcag gacgtcacat atcagaccga
     6181 tcagcaaacc ttgatcctca acaccgcgac cgcttatttc aacgtgttga atgctattga
     6241 cgttctttcc tatacacagg cacaaaaaga agcgatctac cgtcaattag atcaaaccac
     6301 ccaacgtttt aacgtgggcc tggtagcgat aaccgacgtg cagaacgccc gcgcgcagta
     6361 cgataccgtg ctggcgaacg aagtgaccgc acgtaataac cttgataacg cggtagagca
     6421 gctgcgccag atcaccggta actactatcc agaactggca gcgctgaatg tcgaaaactt
     6481 taaaaccgac aaaccacagc cggttaacgc gctgctgaaa gaagccgaaa aacgcaacct
     6541 gtcgctgtta caggcacgct tgagccagga cctggcgcgc gagcaaattc gccaggcgca
     6601 ggatggtcac ttaccgacgc tggatttaac ggcttctacc gggatttctg acacctctta
     6661 tagcggttcg aaaactcgtg gtgccgctgg tacccagtat gacgacagca atatgggcca
     6721 gaacaaagtg ggcctgagct tctcgctgcc gatttatcag ggcggaatgg ttaactcgca
     6781 ggtgaaacag gcccagtaca actttgttgg tgccagcgag caactggaaa gcgcgcatcg
     6841 tagcgtcgtg caaaccgtac gttcctcctt caacaacatt aatgcatcta tcagtagcat
     6901 taacgcctac aaacaagccg tagtttccgc tcaaagctca ttagacgcga tggaagcggg
     6961 ctactcggtc ggtacgcgta ccattgttga tgtgttggat gcaaccacca cgctgtacaa
     7021 cgctaagcaa gagctggcaa atgcgcgtta taactacctg attaatcagc tgaatattaa
     7081 gtcagccctg ggtacgttga acgagcagga tctgctggca ctgaacaatg cgctgagcaa
     7141 accggtttcc actaatccgg aaaacgttgc cccgcaaacg ccggaacaga atgctattgc
     7201 tgatggttat gcgcctgata gcccggcacc cgtcgttcag caaacatccg cacgcactac
     7261 caccagtaac ggtcataacc ctttccgtaa ctgatgacga cgacggggct tcggccccgt
     7321 ctgaacgtaa ggcaacgtaa agatacgggt tatctgccgc attcttcccc cttctcgctt
     7381 caatttcgac cagccatcct ctattctgat gggtatttac cactggtccc ggaagacaaa
     7441 aatgaaacgg acaaaatcca tacgccacgc atcgttccgc aaaaactgga gcgcgcgcca
     7501 tctgacacca gtcgctctcg cggttgccac tgtttttatg ctggctggct gtgaaaagag
     7561 tgatgaaaca gtgtctctct atcaaaatgc tgacgactgt tcagctgcaa acccaggcaa
     7621 aagcgccgaa tgtaccaccg cgtacaacaa tgcgctgaaa gaagccgaac gtactgcgcc
     7681 gaaatacgcc acccgtgaag actgtgttgc tgaatttggt gaaggtcagt gccagcaggc
     7741 accagcccag gctggcatgg caccagaaaa ccaggcgcag gcccagcaat ccagcgggag
     7801 tttctggatg ccgctgatgg ccggttacat gatggggcgt ctgatgggcg gcggcgcggg
     7861 atttgcacag cagccgctgt tctcctcgaa aaacccagcc agcccggctt acggtaaata
     7921 taccgacgcg acgggtaaaa actatggcgc agcccagcca ggccgcacca tgaccgtacc
     7981 gaagacagcc atggcaccaa aaccggcgac caccactacc gttacccgtg gcggttttgg
     8041 tgaatctgtt gccaaacaaa gcactatgca gcgtagcgca actggcacct cttctcgttc
     8101 aatgggtggc tgataccgat ggaaagagtc agtattaccg agcgcccgga ctggcgtgag
     8161 aaagcccacg aatacggttt caattttcac accatgtacg gcgagccgta ctggtgtgaa
     8221 gatgcttact acaagttgac cctcgcccag gttgaaaagc tggaagaagt caccgccgaa
     8281 ctgcaccaga tgtgcctgaa agtggtggaa aaagtgatcg ccagcgatga gctgatgacc
     8341 aaattccgca ttccaaaaca cacctggagt tttgtgcgtc agtcatggct gactcaccag
     8401 ccatcgcttt attcgcgtct tgatctggca tgggatggca ctggcgaacc taaactgctg
     8461 gaaaataacg ccgatacacc aacgtcacta tacgaggcgg cgttctttca gtggatctgg
     8521 ctggaagatc agcttaacgc cggtaacttg ccggagggca gcgaccagtt taacagtctg
     8581 caagaaaagc taatcgatcg ctttgtcgag ttgcgtgaac agtatggctt ccagttgctg
     8641 catctcacct gctgtcgcga cacggtggaa gatcgcggaa ccattcagta tttgcaggac
     8701 tgcgcgacgg aagctgaaat cgctaccgag ttcctctaca ttgacgatat cgggttaggt
     8761 gaaaaaggtc agttcacgga tttacaggat caggtaattt ccaacctgtt caagctgtat
     8821 ccatgggaat ttatgttgcg tgagatgttc tccaccaagc tggaggatgc aggcgtacgc
     8881 tggctggaac cggcgtggaa gagcattatc tccaacaaag cgcttctacc gctactgtgg
     8941 gagatgttcc cgaatcaccc gaacctgctg cccgcttatt ttgcggaaga tgatcatccg
     9001 caaatggaaa aatatgtggt taaaccgatc ttctcccgtg aaggcgcaaa cgtgtcgatc
     9061 attgagaacg gcaaaaccat tgaagcagcg gaaggtccgt atggcgaaga agggatgatt
     9121 gttcagcaat tccacccgtt accgaaattc ggcgacagct atatgctgat tggtagctgg
     9181 ctggtgaacg atcaacctgc cggaattggc attcgtgaag accgtgcgtt gatcacccag
     9241 gatatgtctc ggttttatcc acatattttt attgagtaat caatgatgcc ggatggcatt
     9301 cgccatccgg taattgttaa cctatctgca ccgacagcat actcaggctg cccatttcta
     9361 taccatcgac cggaatggta attggctcct gcccatccca cgcacctaag acatacaaca
     9421 acggcagata gtgctccggc gttgggttcg ataacgcgcc accttcatgg tcgaggtaat
     9481 tcaccagagg atgctgctct accgcccctt gccacgtcag attcgctttc acatactcat
     9541 taaacgacat cgcccaagga tatggtgaac tatcaccgtg ccacttcact gtacgcaggt
     9601 tatgcaccac gttgccactg gcgaccaaca ttattccttc atctcgcagc gctgccagtt
     9661 tgcgccccat ttcgaaatgc caggcggcag gtttgctact gtcgatactc aactgcacca
     9721 tcgggatatc ggcgtcagga tacatcttaa tcagcacgcc ccaggaaccg tggtcaaagc
     9781 cccaggcttc tttatccagc gtcaccggga ccggcgctaa cagctcaacc agatgctgtg
     9841 ccagcatagg cgaacccgga gcaggataat gcgtatcgta cagcgcctgc gggaagccac
     9901 caaagtcatg aatcgtgggc ggcgtctcca tcgcggtcac tcctgttcca cgggtaaacc
     9961 agtgagccga aaccaccaca atcgcttgcg ggcgtggcaa tgtcatcccc aacttctgcc
    10021 agctgcgggt atacaaatta tcttccagca cgttcatcgg actaccgtga cctaaaaaca
    10081 atgctggcat acgtgttgaa gacatgatga tatccttaac taaaggtgtc attttgatat
    10141 cctcacaata cgcttgttcg gcggagtaag aacccggata acaatgatga tgatcatcag
    10201 ttattttgac gatctgcctg aaagtgaaga tttataagga gttgtcgatg tcagtacctc
    10261 tcattctgac catactggcg ggggcagcca cgtttattgg cgcgtttctc ggcgttctcg
    10321 ggcaaaaacc ctcgaaccgc ttactggcgt tttcgctagg atttgcggcg gggatcatgt
    10381 tgctcatctc attaatggaa atgcttcctg ccgcactagc agctgaagga atgtcgcctg
    10441 tgttgggtta tggaatgttt atcttcggtc tgcttggcta ttttggcctg gaccgcatgt
    10501 tgccacatgc gcatccgcag gatttaatgc aaaaatcggt gcagccgttg ccaaaatcga
    10561 tcaagcgcac agccattctg ctcactctcg gcatcagtct gcataacttc ccggaaggga
    10621 ttgccacctt tgtcacggcg agcagcaacc tggagctggg atttggcatc gcactggccg
    10681 tcgctttgca caatatccct gaaggtctgg cagtggcagg cccggtttat gcggcaacgg
    10741 ggtctaaacg caccgcgatt ctgtgggcgg ggatttctgg actagcagaa attcttggtg
    10801 gtgtgctggc gtggttaatc ctcggtagca tgatttcccc ggtggtcatg gcggcaatca
    10861 tggcggcggt tgcaggaatt atggtggcgc tctcggttga tgaattaatg ccgctcgcca
    10921 aagagattga ccctaataat aaccccagct atggcgtact atgtggaatg tcagtgatgg
    10981 gattcagttt agtgctgcta caaacggcgg gaattggtta aaaacaacat cagaagttgt
    11041 ccagcacatg gacaacttct gccagcttca cgaaagcctt attctacctt ttcggctgtc
    11101 tcttctctcg tactgtttaa gtatttgttc cgcgcttcaa gcagtgcgtc tctgtcctgg
    11161 cgaaacgccc gacaataacg tttcaaatgc aggaaataac ccaccacaac cagagcaatc
    11221 aatacaatcc agtaccaggc gataaacatc cttttaccct taatatttaa caatactaaa
    11281 catttagcgc ataaatttca catatccttt ttcggatata ttcatatggt cgcataataa
    11341 cggaacaatt atatgatttt gatcataatg aaaaagaatg cctgatatta atgaaatttt
    11401 ctcatagaaa gagaagcgga attaacatct tgcatagcgg ggcaaaataa aaaccgaagc
    11461 cagctacaac ttcggtttct tgattaaggc agtaattaag cagcggtttt cagctggctt
    11521 tacgctcatg tgcctgacgg tatgccacca ggtcttcaat agtcacgagc gccatattgt
    11581 gtttattggc aaactcaatg cactctggtg cacgcgccat cgtgccatcg tcattagtca
    11641 gctcacacag cacaccagcc ggtttaaagc ctgccagcgt catcaaatca atagtcgctt
    11701 cagtatgacc gccacgcgtc agtacaccac ctgcctgcgc gcgaagtggg aaaacgtggc
    11761 ccgggcgatt cagatcagaa ggttttgcgc catcggcaat cgctgcgcga acggtcgtaa
    11821 tacgatcagc ggcagaaaca ccggtagtca caccttcagc cgcttcaatg gtcacggtaa
    11881 aaccggtgcc ataggcgctg gtgttatttt ctaccatcat tggcagatca agttgtttac
    11941 ggcgatcttc agtgatgcac aggcaaacaa taccgctacc gtggcgaatg gtcagcgcca
    12001 tctgctcaac ggtcatggtt tctgccggga agatcatatc gccttcgttt tcacggtctt
    12061 catcatcaag caccattaca ccgcgtcctt cacgcagcgc agccagtgca ttttcaacac
    12121 gttcgaaagg cgtaccaaaa gaggaaagta gcgtctgatt catggtaaaa aaacctcact
    12181 aaaattatgg ttaccagaat cagggcagtc ttaggagtgg cggcatatag ccaaaataac
    12241 gtgagcgggt ccatgcccga cagaatcgtt actctctccc atccggactc taaccgtcgg
    12301 ccccggaatt acaccggatc tgctgtcctt tgagttcgca cccaaagcgc tcgcgggctt
    12361 tcaactgagt tgatttaccg ccggtgggga atttcgcccc gccctgagaa taagcggatt
    12421 cactataacg ctaatgatta gcggcagcaa cgcatagctt cacataattc tggtttatga
    12481 cttaccctta tcgcactaca atggtactca acacctatca gtacagggaa actacaatga
    12541 ttgacccgaa aaaaattgag caaatcgctc gccaggttca cgaatcaatg cctaaaggaa
    12601 tcagggagtt cggggaagat gtggagaaaa aaatccgcca aaccctacaa gctcagctga
    12661 cgcgcctcga ccttgtaagc cgtgaagagt tcgacgtcca aacgcaagtc ctgttacgga
    12721 ctcgtgagaa actggcgctg cttgaacaaa gaatgagcga actggaaaat cgcagtactg
    12781 agattaaaaa gcagccagat ccagagactc tccccccaac gctgtaaaca tttgtcttta
    12841 atgcactgta aattatgaat taatcctcta atttacagtg ctttctatta ccttatcgct
    12901 taattccatt cagaatatca tcacaaaaat gaatgagtgc aattgaaaat ataattgtta
    12961 tgtatttttt aagttttact taatccatat ttagataaac t
//
LOCUS       DAZGVQ010000143        12759 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000143 DAZGVQ010000000
VERSION     DAZGVQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.144"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(223..2214)
                     /gene="tkt"
                     /locus_tag="ACHLAG_004019"
     CDS             complement(223..2214)
                     /gene="tkt"
                     /locus_tag="ACHLAG_004019"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP0657156.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2492..3250
                     /gene="loiP"
                     /locus_tag="ACHLAG_004020"
                     /gene_synonym="yggG"
     CDS             2492..3250
                     /gene="loiP"
                     /locus_tag="ACHLAG_004020"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFP0657157.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(3306..4049)
                     /locus_tag="ACHLAG_004021"
     CDS             complement(3306..4049)
                     /locus_tag="ACHLAG_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP0657158.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(4036..5145)
                     /gene="dgaE"
                     /locus_tag="ACHLAG_004022"
     CDS             complement(4036..5145)
                     /gene="dgaE"
                     /locus_tag="ACHLAG_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HFP0657159.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGIAIAVAAAITRGDKARVALMPDSSGM
                     ANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGSSNLAALWQLESAITDATAALLYVK
                     SHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEEDLRAWVVSGADMVVYSGAKAFNAP
                     TSGFITGKKIWIAACKAQHHGIARAMKIGKENMVGLVYALENYHQGQAVITAEQLQPV
                     VEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQELGVDARVVEAQLRGGDIAIYARRY
                     NLHQGVFSLDPRTVAEGEMALIVARLKEIADHAKD"
     gene            complement(5149..6009)
                     /locus_tag="ACHLAG_004023"
     CDS             complement(5149..6009)
                     /locus_tag="ACHLAG_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFP0657160.1"
                     /translation="MMSDDVMQHELVERARESGTLTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEEINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(6006..6755)
                     /locus_tag="ACHLAG_004024"
     CDS             complement(6006..6755)
                     /locus_tag="ACHLAG_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP0657161.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPIAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(6781..7266)
                     /locus_tag="ACHLAG_004025"
     CDS             complement(6781..7266)
                     /locus_tag="ACHLAG_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP0657162.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(7277..7705)
                     /locus_tag="ACHLAG_004026"
     CDS             complement(7277..7705)
                     /locus_tag="ACHLAG_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP0657163.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(7824..10622)
                     /gene="dagR"
                     /locus_tag="ACHLAG_004027"
     CDS             complement(7824..10622)
                     /gene="dagR"
                     /locus_tag="ACHLAG_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HFP0657164.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
     gene            complement(10881..11801)
                     /gene="speB"
                     /locus_tag="ACHLAG_004028"
     CDS             complement(10881..11801)
                     /gene="speB"
                     /locus_tag="ACHLAG_004028"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFP0657165.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(11937..12668)
                     /locus_tag="ACHLAG_004029"
     CDS             complement(11937..12668)
                     /locus_tag="ACHLAG_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657166.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         3104 a         3537 c         3209 g         2909 t
ORIGIN      
        1 atgttttgaa aacagtgaat tattaatttc ttatataaca tttagttata gatataacaa
       61 caaatgtcaa tacgcatatc gtgatgcata ttacataatt gatatttatt gcatataaat
      121 atttgtgatc tacaacacgc tttatctatt gcttgtccgc aaacggacat atcaaggtaa
      181 taaaaaaggt cgccgaagcg acctttttac ccgaaatgct aattacagca gttcttttgc
      241 tttcgcaaca acgttatcaa cagtgaagcc gaactcttca aacagcagct ctgccggagc
      301 agattcaccg aaggtggtca taccgacgat agcaccgttc aggccaacat acttgtacca
      361 gtagtcagca atacccgctt ctacagcaac gcgtgcagta accgctttcg gcagtacgga
      421 ttcacggtaa gcagcatcct gcttgtcaaa tgcgtcggta gacggcatgg acaccacgcg
      481 cgctttcacg ccttcggcag tcagtttttc gtaggcagca acagccagtt caacttctga
      541 accggtagcg atgaaaatca gttccggctg accggcgcag tctttcagca cataaccacc
      601 gcgcgcgatg tttgccagtt gctcttcagt tcgttcctgc tgcgccaggt tctgacggga
      661 gaggatcagt gcggtcgggc cgtcctgacg ctcaacaccg tatttccacg cgaccgcgga
      721 ttcaacctgg tcacacggac gccatgtaga catgttcggg gttacgcgca gagaagcgac
      781 ctgctcaacc ggctggtgag tcgggccgtc ttcgcccaga ccgatggagt cgtgggtgta
      841 aaccatcacc tgacgctgtt tcatcagcgc agccatacgt acggcgttac gtgcgtattc
      901 cacgaacatc aggaaggtgg aggtgtacgg caggaaacca ccgtgcaggg agataccgtt
      961 agcaatcgcg gtcataccga actcgcgaac accgtagtgg atgtagttac ccgcagcatc
     1021 ttcgttgatt gctttagaac cagaccacag ggtcaggtta gacggtgcca ggtcagcaga
     1081 accgccgagg aattccggca acagcggacc gaaagcttcg atagcattct gagacgcttt
     1141 acggctggcg attttcgccg ggttagcctg cagtttagca atgaactctt ttgctttcgc
     1201 gtcgaagtca gacggcattt cgcctttcat acggcgggta aattcagcgg cttcctgcgg
     1261 ataagctttc gcgtaagcag cgaatttctc gttccatgcg gattctttcg cctggcctgc
     1321 ttctttcgca tcccactgag catagatttc agacgggatt tcgaacggtg catatttcca
     1381 gcccagctgt tcgcgggtca gggcaatttc agcgtcgccc agcggcgcac cgtgggagtc
     1441 gtgagtaccg gctttgttcg gggaaccgaa accgatgatg gttttgcaca tcagcaggga
     1501 cggtttgtca gtcactgcgc gcgcttcttc tactgcgcgt ttgatggatg ccgcgtcatg
     1561 accgtcgatg tcgcgaataa cgtgccagcc gtaagcttcg aaacgcattg cggtgtcgtc
     1621 ggtgaaccag ccttcaacgt gaccatcaat agagataccg ttgtcatcgt agaacgcaat
     1681 cagtttaccc agcttcagcg tacccgccag agagcaaact tcgtgggaga tgccttccat
     1741 catgcagccg tcgcccatga aggcataggt gtagtggtcg acaatgtcgt ggcccggacg
     1801 gttaaactgc gccgccagcg ttttttctgc aatcgccata ccgactgcgt tggcaatacc
     1861 ctgacccagc ggaccggtgg tggtttccac accagcggtg taacccactt ccgggtgacc
     1921 cggagtttta gagtgcagct gacggaagtt tttcagttct tccatcggca gatcgtaacc
     1981 ggtgaggtgc agcaggctgt agatcagcat ggagccgtgg ccgttggaca gcacgaagcg
     2041 gtcacggtca gcccaggacg gattctgcgg gttgtgtttc aggaaatcac gccacaggac
     2101 ttcggcaatg tcagccatac ccataggggc acccgggtga ccggatttgg ctttctgtac
     2161 tgcgtccatg ctcagcgcac gaatagcatt ggcaagctct ttacgtgagg acattttgac
     2221 tccagatcgg atgatgaagg gcacgccctt aacgacttga cgacagcgcg ttttgggcta
     2281 cgccggaaaa tttgccaaca atttaccgca agccgcgcgt catgtacatg gaacatcctt
     2341 ttgccgcttc agaaatctct ggatcatgct cgcatgttgc gcaatctact cgcccgtccg
     2401 ctgcgctttt ccttatactg agactgagcg tcgattcacc tgcaaacggc gcatttttag
     2461 aataatcctg accttgtgcg gaagagaaaa catgaaaatt cgcgccttat tggtagcaat
     2521 gagcgtggca acggtactga ctggttgcca gaatatggac tccaacggac tgctctcatc
     2581 aggagcggaa gcttttcagg cttacagttt gagtgatgcg caggtgaaaa ccctgagcga
     2641 tcaggcatgt caggagatgg acagcaaggc gacgattgcg ccagccaata gcgaatacgc
     2701 taaacgtctg acaactattg ccaatgcgct aggcaacaat atcaacggtc agccggtgaa
     2761 ttacaaagtg tatatggcga aggatgtgaa cgcctttgca atggctaacg gctgtatccg
     2821 cgtctatagc gggctgatgg atatgatgac ggataacgaa gtcgaagcgg tgatcggtca
     2881 cgagatgggg cacgtggcgt taggccatgt gaagaaaggg atgcaggtgg cgcttggtac
     2941 aaatgccgtg cgagtagctg cggcctctgc gggcgggatt gtcggcagtt tatctcaatc
     3001 acaacttggt gatctgggcg agaaattagt caattcgcaa ttctcccagc gccaggaagc
     3061 agaagccgat gattattctt acgatcttct gcgccaacgc ggcatcagcc cggcaggtct
     3121 tgccaccagc tttgaaaaac tggcaaaact ggaagaaggt cgccaaagct caatgtttga
     3181 cgaccatcct gcatccgccg aacgcgccca gcatattcgc gatcgtatga gcgcggatgg
     3241 ggttaagtaa gtcagacata ccgatctaca acataattgt aggtcggtaa cgcctgaatc
     3301 cccgatcaga gtagtgattt cactttattt agcaaaacgg ctacatcatc cggtcgggta
     3361 tatcccgtat cagggtctat aatcgagctg taaacatgtg gcataatgcg cggcaccccc
     3421 gcctccagac aagttttcag aatcacactg aagttatcca aatcaatccc gccagtcggc
     3481 tcaatcagcg tcataccatt gcgcgccgcc gtggtcgcca gcgcgtatag ctccggaagc
     3541 gacctctcgc cgcccatcgg aaagaacttc gccgcgtgtg cgcccatatc tagcatcatt
     3601 cgcaccgccg tatcgcagga gacgcgggca ggcgtaccct ggctgctgct aacgccggtg
     3661 gaaatcagca cttcgcccgg cgtccccgtc gggctaacca gcgcgttgac atgggtctgc
     3721 tcaccgcccg tagccgcaag cgcgcctgcc gcgaaaccac aaccggtaaa ggtctggtta
     3781 acgtgtgctg gatgaatctg cgccgcaatc atcgcagctt tgtaatactg cgccgggtca
     3841 cccgccccca gacctactga aatggacggt atttccgcca tccaacgttt aacttcctgg
     3901 atcccctcat ccaccgagga gaactgcgcg gaaagcacgc caatcactgc gtggccttcg
     3961 gcggcgtcaa agatttcacg ggcattggcg atatctttcg ccagcacatt aattgccacg
     4021 cgattacgat agaaattaat cttttgcatg atctgcaatc tccttcagtc gcgccacgat
     4081 aagtgccatt tccccttccg ccaccgtacg cggatcaagg ctaaaaacgc cctggtggag
     4141 gttataccgc cgtgcataaa tcgcgatatc gccaccacgc agttgcgctt cgacgacgcg
     4201 agcgtcaact cccaattcct gcgcatttac tcgaatacga atacgccaga ttgcacgccc
     4261 ggcttcatcc tgctcaatat cagccgttaa cccgtgaatc gcagaaatcg cttcaaccac
     4321 tggctggagc tgttccgcgg tgatgactgc ctgcccctgg tgataattct ccagcgcgta
     4381 aaccagccca accatgttct ccttgccgat tttcatcgcc ctggcaatgc cgtgatgctg
     4441 cgctttgcag gctgcaatcc agattttttt accagtgata aacccggagg tcggcgcgtt
     4501 aaaggctttt gcgccgctgt agaccaccat atccgctccg ctcaccaccc aggcacgtaa
     4561 atcctcttcg gcggcggcat cgacaatcag cggcaggttg tacatctgcg cgacatgtgc
     4621 gaagtcctcg atgttcaaca ttcccttctg cacgcagtga tgcgacttca catacagcag
     4681 cgcggcggtc gcgtcggtaa tggcactctc cagctgccag agagccgcca ggttacttga
     4741 acccacctcc acaacgcgcc cgccacctaa gcggatcgcg ctgaggatcg atgcaccgta
     4801 atcgacgtta tggccgcgca gcatcaccac ttcattggcc atgccggagc tatccggcat
     4861 gagcgccacc cgggccttgt cgccgcgtgt aatggcggcg gcgacagcga tggcgatccc
     4921 tgctgaggca caggaggtaa cgtagctatc ctccgccccg gtaaagcgag agaccagctc
     4981 gccggttttg tccaccagct ggtcaatctc cacgaaagag gatgccgcgc gcgcggtggc
     5041 ctgcatcacc tccggcgcga cgctggagac gccgagaatc gtcatcttgc cgcaggcgtt
     5101 aatcacctgt ttcagtccca gttgttgata gatattctgc gtcatggctt acagcactcc
     5161 cagcagcgag cagaccacgc tcaacgccac aatcgacagc agaatcgtgg tgtaacgcgg
     5221 accctttttc accaaataga agtagatggc gaacactgcc gccagcggca gcaggccggg
     5281 cgcgatggag tccagaatct gctgtaccac cacttcagag cctttcagtg cgctaatttt
     5341 taaaggtgag gttatcttga caaagctcgc ggatagcgcg cccatcatga taagccccag
     5401 tacgttagcg ccgtagataa gctccttgat gcgccctccc tgcagcagac tgataatcga
     5461 atcgcgcccc agcgtatagc ccttgtgcac catgccgtag ctgattgcca gcgtaatcgc
     5521 cgggtagaga atcagcggca aaataccgcc catcgcgctg ccattggcgg cgaacgggat
     5581 aaaaatagca atcaacagcg gcatcaccgc cgcccagatg atggagtcgc ccatcccggc
     5641 cagcggcccc atcaggccgg ttttaatccc ggtgatagag gcatcgttaa tctcctcgcc
     5701 gcgggtcttt tgctcttcca tggcgatgga gatcccctgg atcaccgcgc caaacgtctg
     5761 ctctgagttg aagaagttca gatggcgttt taacgcctcc acctgctcct ccttttgcgg
     5821 atagagcttt ttgataatcg gcgtcatcga ggcgcagaaa atcaggctct gtaaacgctc
     5881 ataggagctg gaaacttccg cgcccagcca gtagataaac cacgctttgg tgatatccgc
     5941 tttggtcagc gtcccggact cccgcgcgcg ctcaaccagt tcatgctgca ttacgtcgtc
     6001 gctcattatg ccgctccttc atttttcgcc aggcctttaa tcaggaaggc cacgcaggtg
     6061 ccgaaaatcg ccatcgccat gatgtccacc ttgaggtaca gcaccgcgaa gaatccgccg
     6121 ataaaccacg gcagcaggct ctttttacca atcaccataa tggtgatggc gaagcccagc
     6181 gccgggagaa tcccgcccat gatttcaaac gagtgggtca gccagtgcgg catcagcttg
     6241 aggaaacttt ccactacgtt ctggccgaag tagttggcgg cgaacaccac tgggaaacgt
     6301 agtaccagcc ccagcagcgc cgggtagaga aaggcgcagc gcataatggc tgacatattg
     6361 gccgtttccg cgtgtttatc ggccatatgc acccaggccg catttagcgt gcgacgcaac
     6421 tggtcgagga atacgccgat aacgccaaat ggaatcgcca gtgcaatcgc caggttcggg
     6481 tccatattgg ccttaacggc gataggaatg gcgatacagg ccgccagcgc cgggtctgac
     6541 ggcacattgc cgcctggcgt tgaggtcaca cccagataga ccaactgcat ccccgcgccg
     6601 ataatcatcg ccgcaggcat attgccgagc agcaggccga caaagaccgc caccaccact
     6661 ggctgtaaca gcatggcgga gaaggtatag ccaaggcgca accgggcaaa ccagtaatac
     6721 agccccatca ggcttgcaaa aagtaaggta tccataattt gttatcccat gttcagaggg
     6781 tcagaatttt ttcaggatat cgtccagcga ctgcggttta tcttccggga tggtctggaa
     6841 aacagcctta atgccgcgac ttttcaaatc gttaagaatg ccaacgtctt tttcatccag
     6901 cgttatgttc tggaacaccg ctttacggtt tggcccgccg cctaaaccgc caacctgaat
     6961 atcagccacg tcaaagccct gctgcaccgc ttcctgaatc gccgccagcg acgggaacag
     7021 caccagcacg ttgccgtcgc ccagctggtt ctctttccac gcggccgcga agctctggtt
     7081 gctaaagcag tccactttga tattcggcgg cgcggccatc aggtagatat ttttcataaa
     7141 cggatcggcg tccagctcgt cgctgaccac cgcaatacgg ttggcctgag actgccctac
     7201 ccacttcgtc accacctgtc cgtggataag acggctgtca atgcgacata acacgatgtt
     7261 tgccatacga ttttccttac tgttgtaatt gacgaatatg ggagacgacg tccaggcaac
     7321 tgttgcgccc agcttccacc agatcggcca cgcaggcggt cagtgggcca tgttcgcgct
     7381 tgtccagcgc ctccagcagc agcgaggcat taagcccgga gatcaccgcc accggataat
     7441 cggcgctcag ccgcgccgcc acgttagagg tggtgccgcc gataaaatcg gtcacgataa
     7501 gcgaaccttc cggcatggtt ttcaccacgg cttcgacgcg ctggtaaaac tcgcccagcg
     7561 tatcgacggg catcagcgcg atttccgtca cgccttcgat ttcgcccatc accatgcgca
     7621 gactgttgca gagctgcagc ccccagccgc cgtgagttag cagcagaatt tcaggtaacg
     7681 gatgaatagg tacagttgtg gtcaaaatag cctcctttgc tccagtgttt acatttcatc
     7741 aggagcaaag gacgtgccag aatttgtgtc acgcacagag gaaatgagat tggatggggc
     7801 gttaagcccc gtaaaccagg ggatcagctg tagagcagtt cgtagatata aaaatattct
     7861 gcgtccgata aacggatagc gtaagcctct tcaatcggca aaaaggagga tttgatgacg
     7921 ctaaaggcac gagcgtccag atcaagccgg ttttctagcg ccatctgtaa cggtttgcgg
     7981 ttaatcacga tacgctcgac catgcagcag cagtggatca gaaaacgtag tgtcacctgg
     8041 cggctcggtt tcagggagag cgtgttggtt aagtagttca gaacatcctc gatctctttc
     8101 agaattcgct gcgggttgag caccgaaata tggttgatga tgctctccat ggtcagcgcg
     8161 ctgataaagc gcatggcgct gcgctccatc tccagtcggc gctcgctgct tgagagatcc
     8221 ggcgtcagca ggctgagcac cagctccggc ccttgctcgg aaaacagttc ctccagcgag
     8281 ataaacggta tatccggcag tcccggctgg aaggtgccga cgatccctgc cagccgttca
     8341 ctggcattaa gcgcctgctg aatacgctcc aggctgcgca cctcgttata gtcgagaatc
     8401 accatacgcg tgtcctgcga catcagctcg ccaaagctct cctccagcac ctttttgatc
     8461 ttttccgccg tccccattcc ggttatacat gagataacca gcactttgcc accgctctct
     8521 tgctgcggcg tgcagagccg acaagggata tttttactct gcataagcac agccaattgc
     8581 gatagatcgc cagtttcgta gcttaaatcc agcccaacct ccagcaggct ggtcagagtg
     8641 atatttggca acaacagaac gtcaatctgg aacagcttgc tgatggtgct gccaaaatgg
     8701 accagcgatc cgatatccac catcagtatc agccgctgcc agcaccggtc ctgaatcatc
     8761 cgggtcaggg tttccagggt gtcatgcacc gactgctcaa atggcatatc aattgcgtag
     8821 aacagatcgc gctccagcac ccggttcaca tattgcgcct ggctcgttgc ggtggtcgca
     8881 ccgtgagcga ttagaatcac gccgcaatcc gggctggtgt cgatgcgctg gcggtagtgg
     8941 cgacactctt tcaggaacag gcacagccag accacctccg tcgccgggca ctgaatatgc
     9001 aacaactcat tgattttgcg gcacagaagc gtggcattgt catactcatc tttacagcga
     9061 tccagaatca ggctggatga atagagttga ggtataagcc ctcgccgcac atagccaatc
     9121 agtgcaagaa agtgtttacg taacgggttc gccaggtttt ctggcaatgt aaatcctaat
     9181 acctgctcca cgtaaccgat cagcagcgtc acacgctcct caatttgatc gccatagcgc
     9241 ggtggatgcg ccgcactatg gcggctataa agaccatatt caaaaatcga actgagtttg
     9301 tttttcagga tagccagcgt ttctgcgggc ggcacgttac tgttgcgcag attgacatat
     9361 tcacgagtca ggaaactgta aaacaggtcg ctctcttcta tttccgcgct ggtcgccaac
     9421 gaatttttta acgcgggcaa cgtacgcgca tccacgttca gacgatcttt accgccaaac
     9481 aacgcatcga ccagcagacg ctgctctggc gtggtattaa acgacatttc cgccagtcgt
     9541 ttatctaact ctagcgtatc attacgctcg gtcattcccg acgcccacgc ctgcgcgcac
     9601 aagaactgaa tatcactttt aagctggccg atattgcctt ccaggggttt acttaacaac
     9661 caatgcaaga gcgttttatc aaggcttatg gtacgttcaa ttttacgact ttcacgctga
     9721 aagaagctta caatcaattc gagctgctcc tcgaccgaac gctggcgaat acccggtaag
     9781 tcaatacata cctggatacg ccgctggaag gtacgcaata gtgccgagtt gaccgactct
     9841 gtagtggcgc aaataaggcg caccgaaatt gaatatgttg gcccacttga accaagtggg
     9901 cggtattcac ctttatcgag aatagaaaag agcttctctt gtccttcgta cgggaggcga
     9961 tgcacttcat ccagaagtaa ataacctccg tcagcttgct ccaccaatcc cgttttattc
    10021 tcacttgccc cggtaaatgc ccccttatgg tgaccaaaca gatgtgagga aagcaactcc
    10081 ggattatgag catattcggc acagttaaag taaaccaacg gaggtggcga acctatggtg
    10141 tgcttgcaag cgaagcaatg cattaactca gcaaagaatg ttttgccaac accagatgca
    10201 ccggttaaca gcacatgtag tccgtgggga taaagcaccg ctgcgcggcc tttttctacc
    10261 gcatcacgga gactacggtc gtatccgata agtccggaaa acggatcatc atctgtatat
    10321 ttttctttct ttggcaataa actagccact gaatgaacta tgcattcgca atcatccagt
    10381 tttcgattaa tgagcaattc tagtgcgtga cgatgtagaa aaaaaacagg acgtccctga
    10441 ctttttacca ccaggccttc attccataat tgattaagat ctttactaac cgagttgcgt
    10501 gccagcccaa ggttaaaacc aatcgcctca gcagtaaatg ctttctcctt cgctaaatca
    10561 gacagttcca gcccatacgt cagccgctcc agctccttgt gaataacatc aatccgtctc
    10621 atttctttac cgcttaaaaa acaatagttg ttcagaacat acactatttg taaacgtaag
    10681 aatgagaact atcgggaaga attaatgttc cttgtggcaa tctggtttct gaatattcgt
    10741 gttttaaata ttacttatgg tctgaaacaa ccaggcaggt gaacagagct aagaaaaagt
    10801 agcgtagaaa tataaaaaca tatatcgcac tgatgaatgt ttcccggcat gactatgcac
    10861 accgggaaat atttagtaaa ttactcgccc tttttcgccg cctgaatata cagcatctcc
    10921 agcgccagcg tcgccgcagc cagagcggtg atttccgact gatcatatgc cggagccact
    10981 tccactacgt ccatcccaac gatgttgaga tctttcaggc cgcgtaccag tttaatagcg
    11041 cgatcggagg tcaggccgcc aatcactggc gtaccggtgc ctggtgcaaa agcaggatcc
    11101 aggcagtcga tatcaaaagt caggtaaacc ggcatatcac ccacaatctg tttcacttgg
    11161 gcgataacgt catccacgct gcgatcgttc acctggcagg cgtccagcac ggtaaagccg
    11221 ttgtctttat caaactcggt acgaatacca atctgcacgg aatgattcgg gtcgatcaga
    11281 ccttctttcg gcgcggtata gaacatagtg ccgtggtcaa attcacaacc gttcgcatag
    11341 gtatcggtgt gggcgtcaaa gtgtaccagc gccattttgc cgaaatgctt cgcatgagca
    11401 cgcagcagcg gcagcgtaac aaagtggtca ccaccgaaag agagcatacg cttaccggca
    11461 gccagcagct tctcggcgtg cgcctgcagc ttctcgctca tctcacgggc atcgccgaaa
    11521 gcatatacca gatcaccgca gtccacgacg ttcagacgct cacgcatgtc gaaattccac
    11581 gggaagcggt tgtgttccca ggccagattc gtcgaaacct gacggatagc tgccggaccg
    11641 tggcgaccac ccgcacgacc agaagtggcc atatcgaacg gcacgccagt aatcacccag
    11701 tcagcatcgc tgtcatatgg ctggaagttc atcggcaggc gtaaaaaacc aaaggcgttg
    11761 gaaaccagtg agttatcgta ttgatgacct aaggtgctca tggatggact cctattacaa
    11821 agtcgatata aaaaaaccct tccgcgtcgt taggcccgac gaggaagggt tggatttgcg
    11881 aaacacaagt ttgtgctaat tatcgcctta aatgggcgag gcatcaagcg aggttgctat
    11941 ttccgacacg gtggcggcgc atagccattg ttatctgccg gaaaggcttg tccagagcac
    12001 ggcggcagcg cgttttgcgc cgggccttgt aattcaaagg cggcaaagcc gattaccccg
    12061 gtattacgca catcgccttg cgccgaattg gctgcgtagg aatcagcaac gttggcgaac
    12121 tgaaaagccg cttcagtatg tttatctttg cggaatcctt taatcgctaa cgaatctccg
    12181 gcattcacaa tatagccgtt gttattcagc gaaccttgct tgccgttcag cacatccagg
    12241 ccatcaaccg tagcaacgat ctcgtagttg gtgttacgac tgtaattacg aacatataat
    12301 tgataattca ttccgactct ggcgtggaca atatagcgtg gttcattatt ggatgaatca
    12361 taaacacgcg taataggtag agaattaaaa ttaccatcgc gaatagcata ttccagttcg
    12421 ttaatacgaa tgctataaac gcggtcgtag ccggaaggaa cattagtcga ataactaata
    12481 acgatcatct ccgccggatc gcgccaggct cgctcagcag atacgccctg aaccgttgag
    12541 tgcacatccc caccccaggc ggtgccgaca ttacccgatg cactgctatt atactgcgca
    12601 ttactggaac aaccggccag cagcacgatt aatgccacca acgcgctacg tactttccat
    12661 tttttcataa ccgttccctc tgcatttaat taatgcagcg attgtaggga gattatttaa
    12721 taagaaaagc agctaaatta tttttaagaa tatataaaa
//
LOCUS       DAZGVQ010000144        12621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000144 DAZGVQ010000000
VERSION     DAZGVQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.145"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(84..1367)
                     /gene="gltA"
                     /locus_tag="ACHLAG_004030"
     CDS             complement(84..1367)
                     /gene="gltA"
                     /locus_tag="ACHLAG_004030"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFP0657167.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1573..1680)
                     /locus_tag="ACHLAG_004031"
     CDS             complement(1573..1680)
                     /locus_tag="ACHLAG_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657168.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2061..2465
                     /gene="sdhC"
                     /locus_tag="ACHLAG_004032"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2061..2465
                     /gene="sdhC"
                     /locus_tag="ACHLAG_004032"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP0657169.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2459..2806
                     /gene="sdhD"
                     /locus_tag="ACHLAG_004033"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2459..2806
                     /gene="sdhD"
                     /locus_tag="ACHLAG_004033"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFP0657170.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2806..4572
                     /gene="sdhA"
                     /locus_tag="ACHLAG_004034"
     CDS             2806..4572
                     /gene="sdhA"
                     /locus_tag="ACHLAG_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFP0657171.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4588..5304
                     /gene="sdhB"
                     /locus_tag="ACHLAG_004035"
     CDS             4588..5304
                     /gene="sdhB"
                     /locus_tag="ACHLAG_004035"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFP0657172.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5605..8406
                     /gene="sucA"
                     /locus_tag="ACHLAG_004036"
     CDS             5605..8406
                     /gene="sucA"
                     /locus_tag="ACHLAG_004036"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFP0657173.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8421..9638
                     /gene="odhB"
                     /locus_tag="ACHLAG_004037"
     CDS             8421..9638
                     /gene="odhB"
                     /locus_tag="ACHLAG_004037"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFP0657174.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAADPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9732..10898
                     /gene="sucC"
                     /locus_tag="ACHLAG_004038"
     CDS             9732..10898
                     /gene="sucC"
                     /locus_tag="ACHLAG_004038"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFP0657175.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10898..11767
                     /gene="sucD"
                     /locus_tag="ACHLAG_004039"
     CDS             10898..11767
                     /gene="sucD"
                     /locus_tag="ACHLAG_004039"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFP0657176.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11871..12593)
                     /gene="mngR"
                     /locus_tag="ACHLAG_004040"
     CDS             complement(11871..12593)
                     /gene="mngR"
                     /locus_tag="ACHLAG_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFP0657177.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
BASE COUNT         3032 a         3218 c         3423 g         2948 t
ORIGIN      
        1 acaatttttt gtgtttaggc atataaaaat taacccgcca tttgaacggc gggttaaaat
       61 atttacaact tagcaatcaa ccattaacgc ttgatatcgc ttttaaagtc gcgtttttca
      121 tatcctgtat acagctgacg cggacgggca atcttcatac cgtcactgtg catttcgctc
      181 cagtgggcga tccagccaac ggtacgtgcc attgcgaaaa tgacggtgaa catggaagac
      241 ggaataccca tcgctttcag gatgatacca gagtagaaat cgacgttcgg gtacagtttc
      301 ttctcgataa agtacgggtc gttcagcgcg atgttttcca gctccatagc cacttccagc
      361 aggtcatctt tggtgcccag ctctttcaga acttcatggc aggtttcacg cattacggtg
      421 gcgcgcgggt cgtaattttt gtacacgcgg tgaccgaagc ccatcaggcg gaaagaatca
      481 tttttatctt tcgcacgacg aacaaattcc ggaatgtgtt taacggagct aatttcttcc
      541 agcattttca gcgccgcttc gttagcacca ccgtgcgcag gtccccacag tgaagcaata
      601 cctgctgcga tacaggcaaa cgggttcgca cccgaagagc cagcggtacg cacggtggag
      661 gtagaggcgt tctgttcatg gtcagcgtgc aggatcagaa tacggtccat agcacgttcc
      721 agaatcggat taacttcata cggttcgcac ggcgtggaga acatcatatt caggaagtta
      781 ccggcatagg agagatcgtt gcgcggataa acaaatggct gaccaatgga atacttgtaa
      841 cacatcgcgg ccatggtcgg cattttcgac agcaggcgga acgcggcaat ttcacgatga
      901 cgaggattgt taacatccag cgagtcgtga tagaacgccg ccagcgcgcc ggtaatacca
      961 cacatgactg ccattggatg tgagtcgcga cggaaagcgt ggaacagacg ggtaatctgc
     1021 tcgtggatca tggtatgacg ggtcaccgta gttttaaatt cgtcatactg ttcctgagtc
     1081 ggtttttcac cattcagcag gatgtaacaa acttccaggt agttagaatc ggtcgccagc
     1141 tgatcgatcg ggaaaccgcg gtgcagcaaa ataccttcat caccatcaat aaaagtaatt
     1201 ttagattcgc aggatgcggt tgaagtgaag cctgggtcaa aggtgaacac accttttgaa
     1261 ccgagagtac ggatatcaat aacatcttga cccagcgtgc ctttcagcac atccagttca
     1321 acagctgtgt ccccgttgag ggtgagtttt gcttttgtat cagccattta aggtctcctt
     1381 agcgccttat tacgtaagac tgccggaact taaatttgcc ttcgcacatc aacctggctt
     1441 tacccgtttg ttatttggct cgccgctctg tgaaagaggg gaaaacctgg gtacagagct
     1501 ctgggcgctt gcaggtaaag gatccattga tgacgaataa atggcgaatc aagtacttag
     1561 caatccgaat tattaaactt gtctaccact aataactgtc ccgaatgaat tggtcaatac
     1621 ttccacactg ttacataagt taatcttagg tgaaataccg acttcataac ttttacgcat
     1681 tatatgcttt tcctggtaat gtttgtaaca actttgttga atgattgtca aattagatga
     1741 ttaaaaatta aataaatgtt gttatcgtga cctggatcac tgttcaggat aaaacccgac
     1801 aaactatatg taggttaatt gtaatgattt tgtgaacagc ctatactgcc gccaggtctc
     1861 cggaacaccc tgcaatcccg agccacccag cgttgtaacg tgtcgttttc gcatctggaa
     1921 gcagtgtttt gcatgacgcg cagttataga aaggacgctg tctgacccgc aagcagaccg
     1981 gaggaaggaa atcccgacgt ctccaggtaa cagaaagtta acctctgtgc ccgtagtccc
     2041 cagggaataa taagaacagc atgtgggcgt tattcatgat aagaaatgtg aaaaaacaaa
     2101 gacctgttaa tctggaccta cagaccatcc ggttccccat cacggcgata gcgtccattc
     2161 tccatcgcgt ttccggtgtg atcacctttg ttgcagtggg catcctgctg tggcttctgg
     2221 gtaccagcct ctcttcccct gaaggtttcg agcaagcttc cgcgattatg ggcagcttct
     2281 tcgtcaaatt tatcatgtgg ggcatcctta ccgctctggc gtatcacgtc gtcgtaggta
     2341 ttcgccacat gatgatggat tttggctatc tggaagaaac attcgaagcg ggtaaacgct
     2401 ccgccaaaat ctcctttgtt attactgtcg tgctttcact tctcgcagga gtcctcgtat
     2461 ggtaagcaac gcctccgcat taggacgcaa tggcgtacat gatttcatcc tcgttcgcgc
     2521 taccgctatc gtcctgacgc tctacatcat ttatatggtc ggttttttcg ccaccagtgg
     2581 cgaactgaca tatgaagtct ggatcggttt cttcgcctct gcgttcacca aagtgttcac
     2641 cctgctggcg ctgttttcta tcttgattca tgcctggatc ggcatgtggc aggtgttgac
     2701 cgactacgtt aaaccgctgg cattgcgcct gatgctgcaa ctggtgattg tcgttgcact
     2761 ggtggtttac gtgatttatg gattcgttgt ggtgtggggt gtgtgatgaa attgccagtc
     2821 agagaatttg atgcagttgt gattggtgcc ggtggcgcag gtatgcgcgc ggcgctgcaa
     2881 atttcccaga gcggccagac ctgtgcgctg ctctctaaag tcttcccgac ccgttcccat
     2941 accgtttctg cgcaaggcgg cattaccgtt gcgctgggta atacccatga agataactgg
     3001 gaatggcata tgtacgacac cgtaaaaggg tcggactata tcggtgacca ggacgcgatt
     3061 gaatatatgt gtaaaaccgg gccggaagcg attctggaac tcgaacacat gggcctgccg
     3121 ttctcgcgtc ttgatgatgg tcgtatctat caacgtccgt ttggcggtca gtcgaaaaac
     3181 ttcggcggcg agcaggcggc acgcactgcg gcggcggctg accgtaccgg tcacgcactg
     3241 ttgcacacgc tttatcagca gaacctgaaa aaccacacca ccattttctc cgagtggtat
     3301 gcgctggatc tggtgaaaaa ccaggatggc gcggtggtgg gttgtactgc actgtgcatc
     3361 gaaactggtg aagtggttta tttcaaagcc cgcgctaccg tgctggcgac tggcggggca
     3421 gggcgtattt atcagtccac cactaacgcc cacattaaca ctggcgacgg cgtcggcatg
     3481 gctatccgcg caggcgtacc ggtgcaggat atggaaatgt ggcagttcca cccgaccggt
     3541 attgccggtg cgggcgtact ggtcaccgaa ggttgccgtg gtgaaggcgg ttatctgctg
     3601 aacaaacatg gcgaacgctt tatggagcgt tatgcgccga acgccaaaga cctggcgggc
     3661 cgtgatgtgg tggcgcgttc catcatgatc gaaatccgtg aaggtcgcgg ctgtgatggt
     3721 ccgtgggggc cacacgcgaa actgaaactc gaccacctgg gtaaagaagt tctcgaatcc
     3781 cgcctgccgg gtatcctgga actctcccgt accttcgctc acgttgatcc ggtgaaagag
     3841 ccgattccgg ttatcccaac ctgtcactac atgatgggcg gtattccgac caaagtgacc
     3901 ggtcaggcgc tgacggtgaa cgagaaaggc gaagatgtgg ttgttccggg actgtttgcc
     3961 gttggtgaaa tcgcttgtgt atcggtacac ggcgctaacc gtctgggcgg caactcgctg
     4021 ctggacctgg tggtctttgg tcgtgcagcg ggtctgcatc tgcaagagtc tatcgccgaa
     4081 cagggcgcat tgcgcgatgc cagcgagtct gatgttgaag catctctgga tcgtctgaac
     4141 cgctggaaca ataatcgtaa cggtgaagat ccggtggcga ttcgtaaagc gctgcaagaa
     4201 tgtatgcagc ataacttctc ggtcttccgt gaaggtgatg cgatggcgaa agggcttgag
     4261 cagttgaaag tgatccgcga gcgtctgaaa aatgcccgtc tggatgacac ttccagcgag
     4321 ttcaacaccc agcgcgttga gtgcctggaa ctggataacc tgatggaaac ggcgtatgca
     4381 acggctgttt ctgccaactt ccgtaccgaa agccgtggcg cacatagccg cttcgacttc
     4441 ccggatcgtg atgatgaaaa ctggctgtgc cactccctgt atctgcctga gtcggaatcc
     4501 atgacgcgcc gaagcgtcaa catggaaccg aaactgcgcc cggcattccc gccgaagatt
     4561 cgtacttact aatgcggaga caggaaaatg agactcgagt tttcaattta tcgctataac
     4621 ccggatgttg atgatgctcc gcgtatgcag gattacaccc tggaagcgga agaaggtcgc
     4681 gacatgatgc tgctggatgc gcttatccag ctgaaagaga aagatcccag cctgtcgttc
     4741 cgccgctcct gccgtgaagg tgtgtgcggt tccgacggtc tgaacatgaa cggcaagaat
     4801 ggtctggcct gtattacccc gatttcggca ctcaaccagc cgggcaagaa gattgtgatt
     4861 cgcccgctgc caggtttacc ggtgatccgc gatttggtgg tagacatggg acaattctat
     4921 gcgcaatatg agaaaattaa gccttacctg ttgaataatg gacaaaatcc gccagctcgc
     4981 gagcatttac agatgccaga gcagcgcgaa aaactcgacg ggctgtatga atgtattctc
     5041 tgcgcatgtt gttcaacctc ttgtccgtct ttctggtgga atcccgataa gtttatcggc
     5101 ccggcaggct tgttagcggc atatcgtttc ctgatcgata gccgtgatac cgagactgac
     5161 agccgcctcg acggtttgag cgatgcattc agcgtattcc gctgtcacag catcatgaac
     5221 tgcgtcagtg tatgtccgaa ggggctgaac ccgacgcgcg ccatcggcca tattaagtcg
     5281 atgttgttgc aacgtaatgc gtaaaccgta ggcctgataa gacgcgcaag cgtcgcatca
     5341 ggcaaccagt gccggatgcg gcgtgaacgc cttatccggc ctacaagtca ttacccgtag
     5401 gcctgataag cgcagcgcat caggcgtaac aaagaaatgc aggaaatctt taaaaactgc
     5461 ccctgacact aagacagttt ttaaaggttc cttcgcgagc cactacatag acaagagctc
     5521 gcaagtgaac cccggcacgc acatcactgt gcgtggtagt atccacggcg aagtaagcat
     5581 aaaaaagatg cttaagggat cacgatgcag aacagcgctt tgaaagcctg gttggactct
     5641 tcttacctct ctggcgcaaa ccagagctgg atagaacagc tctatgaaga cttcttaacc
     5701 gatcctgact cggttgacgc taactggcgt tcgacgttcc agcagttacc tggtacggga
     5761 gtcaaaccgg atcaattcca ctctcaaacg cgtgaatatt tccgccgcct ggcgaaagac
     5821 gcttcacgtt actcttcaac gatctccgac cctgacacca atgtgaagca ggttaaagtc
     5881 ctgcagctca ttaacgcata ccgcttccgt ggtcaccagc atgcgaatct cgatccgctg
     5941 ggactgtggc agcaagataa agtggccgat ctggatccgt ctttccacga tctgaccgaa
     6001 gcagacttcc aggaaacctt caacgtcggt tcatttgcca gcggcaaaga aaccatgaaa
     6061 ctcggcgagc tgctggaagc cctcaagcaa acctactgtg gcccgattgg tgccgagtat
     6121 atgcacatta ccagcaccga agaaaaacgc tggatccaac agcgtatcga gtctggtcgc
     6181 gcgactttca atagcgaaga gaaaaaacgc ttcttaagcg aactgaccgc cgctgaaggc
     6241 cttgaacgtt acctcggcgc aaaattccct ggcgcaaaac gcttctcgct ggaaggcggt
     6301 gacgcgttaa tcccgatgct taaagagatg atccgccacg ctggcaacag cggcacccgc
     6361 gaagtggttc ttgggatggc gcaccgtggt cgtctgaacg tgctggtgaa cgtgctgggt
     6421 aaaaaaccgc aagacttgtt cgacgagttt gccggtaaac ataaagaaca cctcggcacg
     6481 ggtgacgtga aataccacat gggcttctcg tctgacttcc agaccgatgg cggcctggtg
     6541 cacctggcgc tggcgtttaa cccgtctcac cttgagattg taagcccggt agttatcggt
     6601 tctgttcgtg cccgtctgga cagacttgat gagccgagca gcaacaaagt gctgccaatc
     6661 accatccacg gtgacgccgc agtgaccggg cagggcgtgg ttcaggaaac tctgaacatg
     6721 tcgaaagcgc gtggttatga agttggcggt acggtacgta tcgttatcaa caaccaggtt
     6781 ggtttcacca cctctaatcc gctggatgct cgttctacac cgtactgtac tgatatcggt
     6841 aagatggttc aggcaccgat tttccacgtt aacgcggatg atccggaagc cgttgccttt
     6901 gtgacccgtc tggcgctcga tttccgtaac acctttaaac gtgatgtctt catcgacctg
     6961 gtgtgctacc gccgtcacgg ccacaacgaa gccgacgagc cgagcgcaac ccagccgctg
     7021 atgtatcaga aaatcaaaaa acatccgaca ccgcgcaaaa tctacgctga caagctggag
     7081 caggaaaaag tcgcgacgct ggaagatgcc accgagatgg ttaacctgta ccgcgatgcg
     7141 ctggatgctg gcgattgcgt agtggcagag tggcgtccga tgaacatgca ctctttcacc
     7201 tggtcgccgt acctcaacca cgaatgggac gaagagtacc cgaacaaagt tgagatgaag
     7261 cgcctgcagg agctggcgaa acgcatcagc acggtgccgg aagcagttga aatgcagtct
     7321 cgcgttgcca agatttatgg cgatcgccag gcgatggctg ccggtgagaa actgttcgac
     7381 tggggcggtg cggaaaacct cgcttacgcc acgctggttg acgaaggcat tccggttcgc
     7441 ctgtcgggtg aagactccgg tcgcggtacc ttcttccacc gccacgcggt gatccacaac
     7501 cagtctaacg gttccactta cacgccgctg caacatattc ataacggcca gggcgcgttc
     7561 cgtgtctggg actccgttct gtctgaagaa gcagtgctgg cgtttgaata cggttatgcc
     7621 accgcagaac cacgcactct gactatctgg gaagcgcagt tcggtgactt cgccaacggt
     7681 gcgcaggtgg ttatcgacca gttcatctcc tctggcgagc agaaatgggg ccggatgtgt
     7741 ggtctggtga tgttgctgcc gcacggttac gaagggcagg ggccggagca ctcctccgcg
     7801 cgtctggaac gttatctgca actttgtgct gagcaaaaca tgcaggtttg cgtaccgtct
     7861 accccggcac aggtttacca catgctgcgt cgtcaggcgc tgcgcgggat gcgtcgtccg
     7921 ctggtcgtga tgtcgccgaa atccctgctg cgtcatccgc tggcggtttc cagcctcgaa
     7981 gaactggcga acggcacctt cctgccagcc atcggtgaaa tcgacgagct tgatccgaag
     8041 ggcgtgaagc gcgtagtaat gtgttctggt aaggtttatt acgacctgct ggaacagcgt
     8101 cgtaagaaca atcaacacga tgtcgccatt gtgcgtatcg agcaactcta cccgttcccg
     8161 cataaagcga tgcaggaagt gttgcagcag tttgctcacg tcaaggattt tgtctggtgc
     8221 caggaagagc cgctcaacca gggcgcatgg tactgcagcc agcatcattt ccgtgaagtg
     8281 attccgtttg gggcttctct gcgttatgca ggccgcccgg cctccgcctc tccggcggta
     8341 gggtatatgt ccgttcacca gaaacagcaa caagatctgg ttaatgacgc gctgaacgtc
     8401 gaataaataa aggatacaca atgagtagcg tagatattct ggtccctgac ctgcctgaat
     8461 ccgtagccga tgccaccgtc gcaacctggc ataaaaaacc cggcgacgca gtcgtacgtg
     8521 atgaagtgct ggtagaaatc gaaactgaca aagtggtact ggaagtaccg gcatcagcag
     8581 acggcattct ggatgcggtt ctggaagatg aaggtacaac ggtaacgtct cgtcagatcc
     8641 ttggtcgcct gcgtgaaggc aacagcgccg gtaaagaaac cagcgccaaa tctgaagaga
     8701 aagcgtccac tccggcgcaa cgccagcagg cgtctctgga agagcaaaac aacgatgcgt
     8761 taagcccggc gatccgtcgc ctgctggctg aacacaatct cgacgccagc gccattaaag
     8821 gcaccggtgt gggtggtcgt ctgactcgtg aagatgtgga aaaacatctg gcgaaagccc
     8881 cggcgaaaga gtctgctccg gcagcggctg atccggcggc gcaaccggct ctggctgcac
     8941 gtagtgaaaa acgtgtcccg atgactcgcc tgcgtaagcg tgtggcagag cgtctgctgg
     9001 aagcgaaaaa ctccaccgcc atgctgacca cgttcaacga agtcaacatg aagccgatta
     9061 tggatctgcg taagcagtac ggtgaagcgt ttgaaaaacg ccacggcatc cgtctgggct
     9121 ttatgtcctt ctacgtgaaa gcggtggttg aagccctgaa acgttacccg gaagtgaacg
     9181 cgtctatcga cggcgatgac gtggtttacc acaactattt cgacgtcagc atggcggttt
     9241 ctacgccgcg cggcctggtg acaccggtac tgcgtgatgt cgataccctc ggcatggcag
     9301 acatcgagaa gaaaatcaaa gagctggcag tcaaaggccg tgacggcaag ctgaccgttg
     9361 aagatctgac cggtggtaac ttcaccatca ccaacggtgg tgtgttcggt tccctgatgt
     9421 ctacgccgat catcaacccg ccgcagagcg caattctggg tatgcacgct atcaaagatc
     9481 gtccgatggc ggtgaatggt caggttgaga tcctgccgat gatgtacctg gcgctgtcct
     9541 acgatcaccg tctgatcgat ggtcgcgaat ccgtgggctt cctggtaacg atcaaagagt
     9601 tgctggaaga tccgacgcgt ctgctgctgg acgtgtagta gtttaagctt cacctgcact
     9661 gtagaccgac gtggataggg tgtcggtcta cggtttaaaa gataacgatt actgaaggat
     9721 ggacagaaca catgaactta catgaatatc aggcaaaaca actttttgcc cgctatggct
     9781 taccagcacc ggtgggttat gcctgtacta ctccgcgcga agcagaagaa gccgcttcaa
     9841 aaatcggtgc cggcccgtgg gtagtgaaat gtcaggttca cgctggtggc cgcggtaaag
     9901 cgggcggtgt gaaagttgta aacagcaaag aagacatccg tgcttttgca gaaaactggc
     9961 tcggcaagcg tctggtaacg tatcaaacag atgccaatgg ccaaccggtt aaccagattc
    10021 tggttgaagc agcgaccgat atcgctaaag agctgtatct cggtgccgtt gtagaccgta
    10081 gttcccgtcg cgtggtcttt atggcctcca ccgaaggcgg cgtggaaatc gaaaaagtgg
    10141 cggaagaaac tccgcacctg atccataaag ttgcgcttga tccgctgaca ggcccgatgc
    10201 cgtatcaggg acgcgagctg gcgttcaaac tgggtctgga aggtaaactg gttcagcagt
    10261 tcaccaaaat cttcatgggc ctggcgacca ttttcctgga gcgcgacctg gcgttgatcg
    10321 aaatcaaccc gctggtcatc accaaacagg gcgatctgat ttgcctcgac ggcaaactgg
    10381 gcgctgacgg caacgcactg ttccgccagc ctgatctgcg cgaaatgcgt gaccagtcgc
    10441 aggaagatcc gcgtgaagca caggctgcac agtgggaact gaactacgtt gcgctggacg
    10501 gtaacatcgg ttgtatggtt aacggcgcag gtctggcgat gggtacgatg gacatcgtta
    10561 aactgcacgg cggcgaaccg gctaacttcc ttgacgttgg cggcggcgca accaaagaac
    10621 gtgtaaccga agcgttcaaa atcatcctct ctgacgacaa agtgaaagcc gttctggtta
    10681 acatcttcgg cggtatcgtt cgttgcgacc tgatcgctga cggtatcatc ggcgcggtag
    10741 cagaagtggg tgttaacgta ccggtcgtgg tacgtctgga aggtaacaac gccgaactcg
    10801 gcgcgaagaa actggctgac agcggcctga atattattgc agcaaaaggt ctgacggatg
    10861 cagctcagca ggttgttgcc gcagtggagg ggaaataatg tccattttaa ttgataaaaa
    10921 caccaaggtt atctgccagg gctttactgg tagccagggg actttccact cagaacaggc
    10981 cattgcatac ggcactaaaa tggttggcgg cgtaacccca ggtaaaggcg gtactaccca
    11041 cctcggcctg ccggtgttca acaccgtgcg tgaagctgtt gctgctactg gcgctaccgc
    11101 ttctgttatc tacgtaccag caccgttctg caaagactcc attctggaag ctattgatgc
    11161 aggcatcaaa ctgattatca ccatcactga aggcatcccg acgctggata tgctgactgt
    11221 gaaagtgaaa ctggacgaag ccggcgttcg tatgatcggc ccgaactgcc cgggcgttat
    11281 cactccgggt gaatgcaaaa tcggtatcca gccgggtcac attcacaaac cgggtaaagt
    11341 gggtatcgtt tcccgttccg gtacactgac ctatgaagcg gttaaacaga ccacggatta
    11401 cggtttcggt cagtcgacct gtgttggtat cggcggtgac ccgatccctg gctctaactt
    11461 tatcgacatt ctcgaaatgt tcgaaaaaga tccgcagacc gaagcgatcg taatgatcgg
    11521 tgagatcggc ggtagcgctg aagaagaagc ggcagcgtac atcaaagagc atgttaccaa
    11581 gccagttgtg ggttacatcg ctggtgtgac tgcgccgaaa ggcaaacgta tgggccacgc
    11641 gggtgccatc attgccggtg ggaaagggac tgcggatgag aaattcgctg ctctggaagc
    11701 cgcaggcgtg aaaaccgttc gcagcctggc ggatatcggt gaagcactga aaactgttct
    11761 gaaataaata tctgtaataa gaaatagccc tcgccgcttc cctctacagg aatggcgaag
    11821 ggctgtcggt ttcgacatgg ttggccatcg tatgatggcc ttttttgtgc ttatcgcgat
    11881 gattttcgtt gcgctatcag ggtaaattta tagtcatcgg tattaaaagc gttgcggcta
    11941 tattcaaaca cccgaccatc aactaaatat ccacgcgata ctttttcaag aatcggcttt
    12001 gtctggctga tattaagcag acggctcatc tcttcggttg gcatcagagg aatgatttcc
    12061 tgttcgctac gatcgataac cattttcttc acttcttcaa taaagtgata ttttgaattt
    12121 tccatgacct gccaggtgag atccgggaac aacgcaagcg gcatccaggt ttcttccagc
    12181 gccattggct tttgcttgcg atagcgcacg cgcttcacat gccacacacg atcctgcgcg
    12241 gtgatttgta gctgttgctg aagaaaatcg tcagccggaa tcacttcaaa tatcaggact
    12301 tcactgtgtg tatcgacgtg acggtccgac agtttttcat caaaactggt taactgaaaa
    12361 atatcgtaat tgacccgctc ttctttgacg taagtcccgc tgccctgaat gctttcgagg
    12421 atctgttgct cgactaactg gcgcaaagcc tgacgcaccg taacccggct gacgccaaac
    12481 tctgtttgta atgccgattc ggtaggtaac gcatcgccag gtttaagctc gccacgcgca
    12541 atttgttcac gaatgcgatc ggcaatctgc cggtataagg gcttgtgtcc catttttagt
    12601 atctcattaa tacgaattta g
//
LOCUS       DAZGVQ010000145        12355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000145 DAZGVQ010000000
VERSION     DAZGVQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.146"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            28..1080
                     /locus_tag="ACHLAG_004041"
     CDS             28..1080
                     /locus_tag="ACHLAG_004041"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFP0657178.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            1156..2589
                     /locus_tag="ACHLAG_004042"
     CDS             1156..2589
                     /locus_tag="ACHLAG_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFP0657179.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            2772..5033
                     /locus_tag="ACHLAG_004043"
     CDS             2772..5033
                     /locus_tag="ACHLAG_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFP0657180.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYKIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(5267..6550)
                     /locus_tag="ACHLAG_004044"
     CDS             complement(5267..6550)
                     /locus_tag="ACHLAG_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFP0657181.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(6702..7178)
                     /locus_tag="ACHLAG_004045"
     CDS             complement(6702..7178)
                     /locus_tag="ACHLAG_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFP0657182.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(7237..8526)
                     /gene="bioA"
                     /locus_tag="ACHLAG_004046"
     CDS             complement(7237..8526)
                     /gene="bioA"
                     /locus_tag="ACHLAG_004046"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFP0657183.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            8613..9653
                     /gene="bioB"
                     /locus_tag="ACHLAG_004047"
     CDS             8613..9653
                     /gene="bioB"
                     /locus_tag="ACHLAG_004047"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFP0657184.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            9650..10804
                     /gene="bioF"
                     /locus_tag="ACHLAG_004048"
     CDS             9650..10804
                     /gene="bioF"
                     /locus_tag="ACHLAG_004048"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFP0657185.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            10791..11546
                     /gene="bioC"
                     /locus_tag="ACHLAG_004049"
     CDS             10791..11546
                     /gene="bioC"
                     /locus_tag="ACHLAG_004049"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFP0657186.1"
                     /translation="MATVNKQAIAAAFGRAAALYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            11539..12216
                     /gene="bioD"
                     /locus_tag="ACHLAG_004050"
     CDS             11539..12216
                     /gene="bioD"
                     /locus_tag="ACHLAG_004050"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP0657187.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         2923 a         3115 c         3369 g         2948 t
ORIGIN      
        1 ttatgttttt aaattgaggg cattattatg aaaaaaatac cctgcgtgat gatgcgaggt
       61 ggaacctcga ggggcgcgtt cttgttagcg gaacatttac ccgaagatca aacgcagcgc
      121 gataaaatat tgatggcaat tatgggttcc ggtaacgatc tggaaattga cggtattggc
      181 ggcggtaatc cgctgaccag taaagtcgcc attattagcc gttccagcga tccgcgtgct
      241 gatgtcgatt atctgtttgc tcaagttatt gtccatgagc aacgtgtcga taccacgcct
      301 aattgtggca atatgctgtc tggtgttggg gcattcgcca ttgaaaatgg tttgattgca
      361 gcgacatcac cggtcacccg tgtgcgtatc cgcaacgtca atacgggtac gttcatcgaa
      421 gctgatgtgc aaacgccaaa tggtgttgtc gagtacgagg gtagtgccag aattgacggc
      481 gtaccgggta ctgccgcacc ggttgcgctc actttcctga atgccgctgg aaccaaaacc
      541 ggaaaagttt tcccgactga taatcagatt gattattttg acgatgtccc ggtgacctgt
      601 atcgatatgg cgatgccagt cgtcattatt ccggctgaat atctgggaaa aacaggttat
      661 gaattaccgg cggaactgga tgccgacaaa gcattattag cccgcattga atctatccgt
      721 ctacaagcgg gtaaagcaat gggcttaggt gatgtcagta atatggttat ccctaaacct
      781 gtgcttattt ctccagcgca gaaaggcggg gcaattaatg tgcgttattt tatgccgcat
      841 tcttgccatc gcgcgctggc gataaccggt gctattgcta tttccagtag ttgtgcattg
      901 gaaggcaccg tcacccgaca aatcgtccct tctgtaggat acggcaatat caatattgaa
      961 caccccagtg gtgcgctcga cgttcattta agtaatgaag gtcaggatgc cacgacgtta
     1021 cgcgcatctg ttattcggac gaccagaaaa atattttccg gtgaagttta tcttccctga
     1081 aaaaattcgt tgtcaagata aggataatca ataaaggact tctgtatgag tcatacagaa
     1141 agaacaggat tttaaatgaa taagaaatcg ttatggaagc taattctgat attagcgatc
     1201 ccatgtatta ttggttttat gccagctccg gcaggattaa gcgaactggc gtgggtgctt
     1261 tttggtattt acctggcggc cattgtgggg ctggttatca agcctttccc ggaacctgtc
     1321 gtactgttaa ttgccgttgc tgcctccatg gtggtggtcg gtaacttatc cgacggtgcg
     1381 tttaagacca ccgccgtatt aagcggttac tcttcaggta ctacctggct ggtgttctcg
     1441 gcgtttacct taagcgccgc atttgtgacc accggtttag gtaaacgtat tgcctatctg
     1501 ctgattggta aaatcggtaa caccacgctg ggtctgggtt acgttacggt attcctcgat
     1561 ctggtactgg ctccggcaac accgtctaac accgcgcgtg cgggcggtat tgtgttaccg
     1621 atcatcaaca gcgtggcggt ggctttgggg tccgaaccgg aaaaaagtcc gcgtcgtgtc
     1681 ggacattacc tgatgatgtc catttacatg gtcaccaaaa ccaccagcta tatgttcttt
     1741 accgcaatgg cggggaacat tctggcgctg aaaatgatca acgacattct gcacctgcaa
     1801 attagctggg gtggatgggc actggccgcc ggattgccgg gcatcattat gctgctggtc
     1861 accccactgg tgatttatac catgtatccg ccagaaatta agaaggtgga taataaaacc
     1921 atcgctaaag cgggccttgc cgaactggga ccgatgaaaa tccgcgaaaa aatgctgctc
     1981 ggtgtctttg tgctggcgct actgggctgg attttcagta agtctctggg ggttgatgaa
     2041 tccactgtgg caatcgttgt tatggcgacc atgctgctgc tgggtatcgt tacctgggaa
     2101 gacgtggtta aaaataaagg cggctggaat accttaatct ggtacggcgg tattatcgga
     2161 ttaagctcct tattatcgaa agttaaattc ttcgaatggt tagctgaagt ctttaaaaat
     2221 aacctggcat ttgatggtca cggtaacgtt gctttcttcg ttattatttt cctcagcatt
     2281 atcgtgcgtt atttcttcgc ttccggtagt gcctatatcg ttgctatgtt accggtattt
     2341 gccatgctgg cgaacgtctc cggcgcaccg ttaatgttaa ccgcgctggc actgttgttc
     2401 tccaactcct atggcggcat ggttactcac tatggcggcg cggcaggtcc ggtcatcttt
     2461 ggcgtgggtt ataacgatat taaatcctgg tggttggtcg gtgcggtact gacgatatta
     2521 accttcctgg tgcatatcac cctcggcgtg tggtggtgga atatgctgat cggctggaac
     2581 atgctgtaaa tattctcgtc atacttcaag ttgcatgtgc tgcgtctgcg ttcgctcacc
     2641 ccagtcactt acttatgtaa gctcctgggg attcactcgc ttgtcgcctt cctgcaactc
     2701 gaattattta gagtatatcc atttattatc tttctgcgca cttcacggtg cgcagatatc
     2761 tggagcattt gatgatcaag ttatctgaaa aaggcgtgtt tctcgccagt aataacgaaa
     2821 taattgccga agaacatttc accggcgaaa ttaaaaaaga agaagccaaa aaaggcacta
     2881 ttgcctggtc tattctctct tcgcataata cgtccggaaa tatggataaa cttaaaatta
     2941 agtttgattc attagcctct cacgatatta cctttgttgg tattgtacag accgctaaag
     3001 cgtccggtat ggaacgtttc ccgctgccgt atgtgctgac caactgccat aactcactct
     3061 gcgccgtcgg cggcactatt aacggtgatg accatgtttt tggtttatcg gcggcccagc
     3121 gttatggcgg tatttttgtg cctccgcata ttgcggtcat ccatcaatat atgcgtgaga
     3181 tgatggcagg cggcggcaaa atgatcctcg ggtcagacag ccacacccgt tacggtgcat
     3241 tagggacaat ggcagtcggt gagggcggcg gtgagttggt aaaacagctg cttaatgaca
     3301 cctgggatat cgactatccg ggcgtggttg cggtgcatct gaccggaaaa cctgcgccgt
     3361 atgtggggcc acaggatgtg gcgctggcta tcattggcgc ggtgttcaaa aacggttacg
     3421 tcaaaaacaa agtcatggag ttcgttggac cgggcgttag cgcgctctct accgatttcc
     3481 gtaacagcgt tgacgtgatg accacggaaa ccacctgttt aagctccgtc tggcaaactg
     3541 atgaagaagt gcataactgg ctggcgctgc acggtcgcgg ccaggattac tgccagctta
     3601 accctcaacc gatggcgtac tacgatggct gcatcagcgt tgatttaagc gccatcaaac
     3661 caatgattgc gctgccgttc cacccgagca acgtgtataa aatcgacaca ctgaaccaga
     3721 acctgaccga cattctgcgt gagattgaaa ttgagtccga acgcgtggcg cacggtaaag
     3781 ccaaactctc gctgctggat aaagtggaaa atggtcgcct gaaagtgcag caggggatta
     3841 tcgcgggctg ttctggcggt aactacgaaa acgtcatcgc ggcggcgaat gcactgcgcg
     3901 gtcaatcctg tggcaatgac accttctcgc tggcagttta cccgtcatca cagccggtgt
     3961 ttatggatct cgccaaaaaa ggtgtggtag cagatttgat tggcgcaggc gcaatcatca
     4021 gaaccgcgtt ctgcggccca tgctttggcg cgggcgatac gccaatcaac aacggtttga
     4081 gtattcgcca caccacgcgt aacttcccga accgcgaagg ctctaagcca gctaatgggc
     4141 agatgtcagc ggtggcgttg atggacgctc gttctatcgc tgcgactgcg gcaaacggtg
     4201 gctatttaac ctctgccagc gaacttgatt gctgggacaa cgtgccggag tacgccttcg
     4261 atgtaacgcc gtataaaaac cgtgtttatc agggctttgt gaaaggggca actcagcaac
     4321 cgctgattta cggaccgaac attaaagact ggccggaatt gggtgcgctg actgacaata
     4381 tcgtcctgaa agtgtgctcg aagatcctcg acgaagtgac caccaccgac gaactgattc
     4441 cttccggtga aacctcttct tatcgttcaa atccgattgg tctggcggag tttaccctgt
     4501 cacgccgcga tcccggttat gttggcagaa gtaaagcgac tgctgagctg gaaaatcagc
     4561 gtctggcggg gaatgtcagc gagctgacag aggtgtttgc gcgcattaag cagattgctg
     4621 gtcaggagca tattgatccg ctgcaaactg aaattggcag catggtatat gcggtgaaac
     4681 caggcgatgg ttctgcgcgt gaacaggcgg cgagctgcca gcgtgtgatt ggcggtctgg
     4741 cgaatattgc cgaagagtac gcgactaaac gctaccgttc taacgtcatc aactggggga
     4801 tgttaccgct gcagatggcg gaagtgccaa cctttgaagt gggggattac atttacatcc
     4861 ctggcattaa agcggcgctg gataatccgg gtacgacgtt taaaggttat gtgatccatg
     4921 aagatgcgcc ggtaacggaa attacgctct atatggaaag tctgactgct gaagagcgcg
     4981 agattatcaa ggcgggtagt ttgattaact tcaataaaaa ccgtcagatg taaaaagcgc
     5041 catgtgaatg taggtcgcat tcggcactta ttgtcggatg cgatgcttgc gcatcttatc
     5101 cgacctacga atcgtatcga atctgtaggc cagataaggc attttcgcag catccggcac
     5161 ttattgtcgg atgcgatgct tgcgcatctt atccgaccta caaatcgcat cgaaccgtag
     5221 gccggataag gcgtttacgc cgcatccggc aaatagttaa ttgctcttac ttcttcgcct
     5281 ctgcaaccac tttactaccc acgccgcggt tattgtattc ccacatgcgg ttgtagttag
     5341 tgtcattcag attgcgctgt atttcgtcgt tatcatctac gctgccggta ttacccgcaa
     5401 acggacgatt agagatcacc gcatcggccc acggtttagc cgtgttaaaa ccttcgttga
     5461 tggcgctatc acggatcacc acctgaccgt tggtattggc atcaacatcc agcgagcggc
     5521 ccagttgcgc cacaccatca ccgaaagcat tgaaacggct gtttacggcg aggaaaccgt
     5581 agtaaatgtt ggacagcgta gccggtgcaa acacatacgc ttcttgctga gtacgtgagt
     5641 tcaccacgcg gaattcggtg ttatcgaaca ccactgcgcc gcgaccagaa acgatatcca
     5701 catccccttc aatgtagctg ttggtcacca gcgtacgcgg ctgacggttg gtttccagac
     5761 ggttctgcac accgctgttg gtgacaaaga aggtgttctg acgaccgaga atgttaacgt
     5821 tgttaatctg tacctggtca ccatcagtac gcagtgccac cgccggatgg ttacctgcat
     5881 ctacgctatc gcccagcgtg ttttcgatgg tcagattttg cagttgcagg ccattgtttt
     5941 gtgaccagaa gaccgcagag cagagaacac cgatactgtc gctgcgtttg ctctggcagc
     6001 tatcgtacat ataccacgct ggtttacctg gcatatattt gccgcgcggg ttgacgtcgt
     6061 gacgccagtc ggcagggctc atgccaccat caagggaaag cccaatcttc acatcaatcg
     6121 gtttttcacc tgtaccgtac agagtaattc cacccggagc ggcagggaca tataccgttc
     6181 cctgatactc accaggcatc acggcaatat actggcgctt gttggtacgc ttgataattg
     6241 ccgcatctac cgccgcctga atcgtggtat gcgttacacc ttgagtgccc gccgggccga
     6301 caacaaagtc aggttgcgca ggcagggtaa tcggggaagg attccacgct gcagcacctg
     6361 gtgtcaggga tgcaaaatag tgttgagcat cgaaattctg cgcttctttt gccgacagaa
     6421 tcgggcgaga agaggtacca ggcgcggttt gatcagaagg acgttgatcg ggcggggttg
     6481 agctacaggc ggtcagcgtc acgccaaaag ccaatgccag cgccagacgg gaaactgaaa
     6541 atgtgttcac aggttgctcc gggctatgaa atagaaaaat gaatccgttg aagcctgctt
     6601 ttttatacta acttgagcga aacgggaagg taaaaagaca aaaagttgtt tttaatacct
     6661 ttaagtgata ccagatggca ttgcgccatc tggcagagtg attaactaaa catcgcagta
     6721 atcgaggcgc ttgccagaga gtggaaatga acgttaaacc cgaccatcgc gccgctggca
     6781 ccttcatcga catcaatacg ttctacatcc agcgcgtgaa cggtaaaaat gtagcgatga
     6841 gtttcgcctt tcggcggtgc tgcgccatcg tacccggttt taccaaagtc ggtacgcgtc
     6901 tgcaaaacgc cgtctggcat tgctaccaga ccagagccaa acccttgcgg taatacgcgg
     6961 gtatcagcgg gtaagttaac aactacccag tgccaccagc cggagccggt tggcgcatcc
     7021 gggtcgtagc aggtgacaac aaaacttttc gttcccgcag gaacatcatc ccacgccaga
     7081 tgcggtgaaa tattatcgcc atcgtaaccc atgccgttaa agacatgacg atgcggcaat
     7141 ttatcgccat cgcgcagatc gttactgatg agtttcatga accctccttt cttgtttgca
     7201 gaaagtgtag ccagaaaccc tcacgcggac ttctcgttat tggcaaaaaa atgtttcatc
     7261 ctgtaccgcg cggttaaccg ctgcggtcag acgctgcaac tgttgcggga gaataatata
     7321 gggcggcatc aggtaaatca gtttgccaaa aggccggatc cagacaccct gttcgacaaa
     7381 gaatttttgc agcgccgcca tattcaccgg atgagtggtt tcgactacgc cgattgcccc
     7441 cagcacgcgc acatcggcaa ccatttcggc atcacgggcg ggggcaagtt gctcgcgcag
     7501 ctgtacttca atagccgcca cctgttgctg ccagtcgcca gattcgagaa tcgccaggct
     7561 ggcgtttgct gccgcgcagg ccagcggatt gcccataaaa gttggcccat gcataaagca
     7621 accggcttcg ccgttactga tggtttccgc aacctcgcgc gtggtgagcg tggcggaaag
     7681 ggtcattgtg ccgccggtta aggctttacc gaggcacaaa atgtccggcg cgatttctgc
     7741 atgttcacag gcaaacagtt tgccggtacg accaaatccg gtggcgatct cgtcggcaat
     7801 cagcaagata ccttcgcgat cgcacatttt gcggattcgt tttaaccatt ccggatggta
     7861 catgcgcatc ccgcctgcgc cctggacaat cggttcaatg atcaccgccg cgatttcatg
     7921 acgatgcgcc gccatcaggc gggcaaagcc aaccatatca cgttcatccc attcaccatc
     7981 catgcggctt tgcggggcgg gagcaaacaa gttttctggc agatagcctt tccacaggct
     8041 gtgcattgag ttgtccggat cgcacaccga catcgcgcca aaggtatcgc catgataacc
     8101 attgcggaag gtcagaaaac gctggcgcgc ttcgcctttg gcctgccagt actgcaacgc
     8161 cattttcatc gccacttcca ccgctacgga gccggagtcc gcgagaaaaa cgcattccag
     8221 cggttgcggc gtcattgcca ccagtttgcg gcatagctcg atggcgggcg catgggtgat
     8281 accgccaaac atcacatgcg acatagcatc aatttgcgac ttcatcgccg cattaagctg
     8341 cgggtgatta tagccgtgga tcgccgccca ccaggacgac ataccgtcaa ccaggcgtct
     8401 gccgtcagac aaaattagct cgcaaccttc ggcgctcgcg accggataaa ccggcagagg
     8461 ggaggtcatg gatgtgtatg ggtgccagat atggcgttgg tcaaaggcaa gatcgtccgt
     8521 tgtcataatc gacttgtaaa ccaaattgaa aagatttagg tttacaagtc tacaccgaat
     8581 taacaacaaa aaacacgttt tggagaagcc ccatggctca ccgcccacgc tggacattgt
     8641 cgcaagtcac agaattattt gaaaaaccgt tgctggatct gctgtttgaa gcgcagcagg
     8701 tgcatcgaca gcatttcgat cctcgtcagg tgcaggtcag cactttgctg tcgattaaga
     8761 ccggagcttg tccggaagat tgcaaatact gcccgcaaag ctcgcgctac aaaaccgggc
     8821 tggaagccga gcggttgatg gaagttgaac aggtgctgga gtcggcgcgc aaagcgaaag
     8881 ctgcgggatc gacgcgtttc tgtatgggcg cggcgtggaa gaatccccac gaacgcgata
     8941 tgccgtacct ggaacaaatg gtgcaggggg taaaagcgat ggggctggag gcgtgtatga
     9001 cgctgggcac gttgagtgaa tctcaggcgc agcgcctcgc gaacgccggg ctggattact
     9061 acaaccacaa cctggacacc tcgccggagt tttacggcaa tatcatcacc acccgcactt
     9121 atcaggaacg cctcgatacg ctggaaaaag tgcgcgatgc cgggatcaaa gtctgttctg
     9181 gcggcattgt gggcttaggc gaaacggtaa aagatcgcgc cggattattg ctacaactgg
     9241 caaacctgcc gacgccgccg gaaagcgtac caatcaacat gctggtgaag gtgaaaggca
     9301 cgccgctggc ggataacgat gatgtcgatg cctttgattt tattcgcacc attgcggtcg
     9361 cgcggatcat gatgccgacc tcttacgtgc gcctttctgc cggacgcgag cagatgaacg
     9421 aacagactca ggcgatgtgc tttatggcag gcgcaaactc gattttctac ggttgcaaac
     9481 tgctaaccac gccgaatccg gaagaagata aagacctgca actgtttcgc aaactggggc
     9541 taaatccgca gcaaactgcc gtgctggcag gggataacga acaacagcag cgtctggagc
     9601 aggcactgat gaccccggac actgacgaat attacaacgc ggcagcacta tgagctggca
     9661 ggagaaaatc aacgcggcgc tcgatgcgcg gcgtgctgcc gatgccctgc gtcgccgtta
     9721 tccagtggcg caaggagccg gacgctggct ggtggcggat gatcgccagt atctgaactt
     9781 ttccagtaac gattatttag gtttaagcca tcatccgcaa attatccgtg cctggcagca
     9841 gggtgcggag caatttggcg tcggtagcgg cggttccggt cacgtcagcg gttatagcgt
     9901 tgcgcatcag gcgctggaag aagaactggc cgagtggctg ggctattcgc gggcactgct
     9961 atttatctct ggttttgccg ctaatcaggc ggtcattgcc gcgatgatgg caaaagagga
    10021 ccgcattgtc gctgaccggc ttagccatgc ctcattgctg gaagctgcca gtttaagccc
    10081 gtcgcagctt cgccgttttg ctcataacga tgtcactcat ttggcgcgat tgcttgcttc
    10141 cccctgtccg gggcagcaac tggtggtgac agaaggcgtg ttcagcatgg acggcgatag
    10201 tgcaccactg gcggaaatcc agcaggtaac gcaacagcac aatggctggt tgatggtcga
    10261 tgatgcccac ggcacgggcg ttatcgggga gcaggggcgc ggcagctgct ggctgcaaaa
    10321 ggtaaaacca gaattgctgg tagtgacttt tggcaaagga tttggcgtca gtggggctgc
    10381 ggtgctttgc tcaagtacgg tggcggatta tctgctgcaa ttcgcccgcc accttatcta
    10441 cagcaccagt atgccgcccg ctcaggcgca ggcattacgt gcgtcactgg cggtcattcg
    10501 ccgtgatgag ggggatgcac ggcgcgaaaa actggtgtca ctcattgcgc gttttcgtgc
    10561 cggagtacag gatttgccgt ttacgctggc tgattcatgc agcgccatcc agccattgat
    10621 cgtcggtgat aacagccgtg cgttacaact ggcagaaaaa ctgcgccagc aaggctgctg
    10681 ggtcacggcg attcgcccgc caaccgtacc cgctggtact gcgcgactgc gcttaacgct
    10741 aaccgcagcg catgaaatgc aggatatcga ccgtctgctg gaggtgctgc atggcaacgg
    10801 ttaataaaca agccattgca gcggcatttg gtcgggcagc cgcactctat gagcaacacg
    10861 cagatctaca gcgccagagt gctgacgtct tactggcaat gctcccacag cgtaaataca
    10921 cccacgtact ggacgcgggt tgtggacctg gctggatgag ccgccactgg cgggaacgtc
    10981 acgcgcaggt gacggcctta gatctctcgc cgccaatgct tgttcaggca cgccagaagg
    11041 atgccgcaga ccattatctg gcgggagata tcgaatccct gccgttagcg actgcgacgt
    11101 tcgatcttgc atggagcaat ctcgcagtgc agtggtgcgg taatttatcc acggcactcc
    11161 gcgagctgta tcgggtggtg cgccccggag gcgtggtggc gtttaccacg ctggtgcagg
    11221 gatcgttacc cgaactgcat caggcgtggc aggcggtgga cgagcgtccg catgctaatc
    11281 gctttttacc gccagatgaa atcgaacagt cgctgaacgg cgtgcattat caacatcata
    11341 ttcagcccat cacactatgg tttgatgatg cgctcagtgc catgcgttcg ctgaaaggca
    11401 tcggtgctac gcatcttcat gaagggcgcg acccgcgaat attaacgcgt tcgcaattgc
    11461 agcgattgca actggcctgg ccgcaacaac aggggcgata tcctctgacg tatcatcttt
    11521 ttttgggagt gattgctcgt gagtaaacgt tattttgtca ccggaacgga taccgaagtg
    11581 gggaaaaccg tcgccagttg tgcactttta caagccgcga aggcagtagg ttatcggacg
    11641 gcaggttata aaccggtcgc ctctggcagc gaaaagaccc cggaaggctt acgtaatagt
    11701 gacgcgctgg cgttacagca caacagcagc ctgcagctgg attacgcaac ggtaaatcct
    11761 tacaccttcg cagaacccac ctcgccgcac atcatcagcg cgcaagaggg caggccgata
    11821 gaatcattgg taatgagcgc cggattacgc gcgcttgaac aacaggctga ctgggtgtta
    11881 gtggaaggtg ctggcggctg gtttacgccg ctttctgaca ctttcacttt tgcagattgg
    11941 gtaacacagg aacaactgcc ggtgatactg gtagttggtg tgaaactcgg ctgtattaat
    12001 cacgcgatgt tgactgcgca ggcaatacaa cacgccggac tgactctggc gggttgggtg
    12061 gcgaacgatg ttacgcctcc gggaaaacgt cacgctgaat atatgaccac gctcacccgc
    12121 atgattcccg cgccgctgct gggagagatc ccctggcttg cagaaaatcc agaaaatgcg
    12181 gcaaccggaa agtacataaa ccttgccttg ttgtagctat cctgtatttg gttaaattgc
    12241 gagcgagatc gcgtcttcga ttgactgcaa tttaaccaat taaattctaa aataatcacg
    12301 aaaaaaattt tacttccgcc tcatgcggcg aatgtgggaa ttgcccaggc ggcgg
//
LOCUS       DAZGVQ010000146        12340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000146 DAZGVQ010000000
VERSION     DAZGVQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.147"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..142
                     /locus_tag="ACHLAG_004051"
     CDS             <1..142
                     /locus_tag="ACHLAG_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657188.1"
                     /translation="VDHPENKYKDFKDLEDKVEKRLQNRQTEYQNEINQLSAPGVNFD
                     DI"
     gene            complement(324..1079)
                     /gene="mlaA"
                     /locus_tag="ACHLAG_004052"
     CDS             complement(324..1079)
                     /gene="mlaA"
                     /locus_tag="ACHLAG_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFP0657189.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            1373..2305
                     /gene="yfdC"
                     /locus_tag="ACHLAG_004053"
     CDS             1373..2305
                     /gene="yfdC"
                     /locus_tag="ACHLAG_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP0657190.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2381..2455
                     /locus_tag="ACHLAG_004054"
     tRNA            2381..2455
                     /locus_tag="ACHLAG_004054"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2414..2416,aa:Arg,seq:cct)
     gene            complement(2897..4144)
                     /locus_tag="ACHLAG_004055"
     CDS             complement(2897..4144)
                     /locus_tag="ACHLAG_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFP0657191.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYSGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(4216..5130)
                     /locus_tag="ACHLAG_004056"
     CDS             complement(4216..5130)
                     /locus_tag="ACHLAG_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFP0657192.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            5346..6779
                     /gene="cscA"
                     /locus_tag="ACHLAG_004057"
     CDS             5346..6779
                     /gene="cscA"
                     /locus_tag="ACHLAG_004057"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFP0657193.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(6787..7782)
                     /locus_tag="ACHLAG_004058"
     CDS             complement(6787..7782)
                     /locus_tag="ACHLAG_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0657194.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKPGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            8025..8243
                     /locus_tag="ACHLAG_004059"
                     /pseudo
     CDS             8025..8243
                     /locus_tag="ACHLAG_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            8261..9589
                     /gene="dsdA"
                     /locus_tag="ACHLAG_004060"
     CDS             8261..9589
                     /gene="dsdA"
                     /locus_tag="ACHLAG_004060"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFP0657195.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(9697..11235)
                     /gene="emrY"
                     /locus_tag="ACHLAG_004061"
     CDS             complement(9697..11235)
                     /gene="emrY"
                     /locus_tag="ACHLAG_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFP0657196.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAHIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(11235..>12340)
                     /gene="emrK"
                     /locus_tag="ACHLAG_004062"
     CDS             complement(11235..>12340)
                     /gene="emrK"
                     /locus_tag="ACHLAG_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFP0657197.1"
                     /translation="AVVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGS
                     VTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEV
                     ASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKAL
                     IMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMA
                     VVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLL
                     PAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPELAST
                     VTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         3473 a         2993 c         2804 g         3070 t
ORIGIN      
        1 cgttgaccat cctgaaaata aatacaaaga ctttaaggat ctcgaagata aagttgaaaa
       61 aaggcttcaa aatcgccaga cggaatatca aaatgaaatt aatcaactat ctgcgccagg
      121 tgttaatttt gatgatattt aaaattattt ttccagtttt ttgatttttc aactcctgaa
      181 tatcggaaat agtcagtgac gaacatcagt atttttacat cattacgtcc gggtcattgt
      241 cggtttatct ccttttactt gtggtaattg agggataaca acaaaaaagg tgagtattgc
      301 gactcacctt ttttatttgt ttcttattca gaatcgatat cttttaaatc atcctgaatc
      361 gcttgcgcgt tcggattttc ctgcggtttg agttcgccgc cattagcgat gaaatcatga
      421 cgctggaagt acgcttcgcg caccataata taaggatcgg acgactgacg cagcagacca
      481 tcggaatcca acaactgcgc acgagtttct atcccttcaa gcgtccattt acccacagac
      541 atcggccagg tcagccagga aagaaccggg taaagaccat ccgccatatc accaccgtca
      601 tcacgcagcg tgaagctacc gtagaacggt aactggacgt aaggcccata acccacgcca
      661 taatgaccaa gcgtactacc gaagcggtga ggttcagtcc gttgcagttt cgggttcgcc
      721 atccctgcaa catcaataaa accgcccatc cccaaaatgg tgttcaggaa aaagcgggta
      781 aagtggacca tcccctgata agggtcgccc tgcaagaagt agttaaccat caccgcaggt
      841 tcttcaaggt tgccagtaaa gttgctcaaa ccgttacgcg ccggttgcgg aacataatca
      901 cgccaggcga cagcgaccgg tcgaacaata tacgggtcta atacattgaa gttgaagttg
      961 tacatggtgc ggttgaaccc ttctaacggg tcagaacgcc cttgctgatc tgtaccggaa
     1021 ctcgcacacc ccaccagaag cgtagttccc agagcaagcg ccgacaggcg aagcttcata
     1081 aatgtctccc tgttttttta tggcttatgc agtttgccat ccatgacgga acgataccgt
     1141 atccgcctgt ttaggtgtgg gcgattgtaa cagcacgtca actgatgtcc agacgccctg
     1201 atttgctgat ttgatcatag cctggtaatc gccgccctgt aggctacttg attctataga
     1261 aacagaaaaa ggcaaacgca gccttttcta cattttcaga gtaactcccg ccagttgcga
     1321 gcaaaaaagc cgctacgctt tagctatacg tgctaattca agagaagaga ccatggacaa
     1381 cgacaaaatt gatcaacaca gcgacgaaat tgaagttgag agcgaagaaa aagagcgcgg
     1441 caaaaaaata gaaatagatg aagaccgact cccctcccgg gcgatggcaa ttcatgagca
     1501 tatccgccag gatggtgaaa aagagctgga acgcgacgca atggcgctac tgtggtcagc
     1561 cattgcggcg ggtctgtcga tgggcgcttc gctactggca aaagggatat ttcatgtcga
     1621 actggaagga gtgccaggca gcttcttact ggagaatctc ggttatacct ttggttttat
     1681 tatcgtcatt atggcccgcc agcaattatt taccgaaaac accgtgactg cggtactacc
     1741 cgtcatgcaa aaaccgacaa tgagcaacgt cggcttactt atgcgattat ggggcgtcgt
     1801 gctgctgggt aatattctcg ggacaggtat tgctgcatgg gcatttgaat atatgcctat
     1861 cttcaatgaa gaaactcgcg atgcatttgt caaaatcggc atggatgtga tgaagaacac
     1921 ccccagcgag atgtttgcca acgcgatcat ttccggctgg ctgatcgcca ctatggtttg
     1981 gatgtttcct gcagcgggtg cggcaaagat tgtggtgatt attttgatga cctggcttat
     2041 tgcactgggt gacaccaccc acatcgttgt cggttctgtt gaaatcctct atctggtgtt
     2101 taacggcacg ctgcactgga gcgatttcat ctggcccttc gcactaccta ctttagcggg
     2161 gaacatctgc ggcggcacct ttatcttcgc gttaatgagt catgcacaga ttcgtaacga
     2221 catgagcaac aagcgtaaag cagaagcacg ccaaaaagca gaacgtgcgg aaaacattaa
     2281 gaaaaatgat aaaaacccag cataaatggc gagggtttaa gcaatcgagc ggcagcgtac
     2341 ttaccccgca gtccattagc gggtatactc atgccgcatt gtcctcttag ttaaatggat
     2401 ataacgagcc cctcctaagg gctaattgca ggttcgattc ctgcagggga caccagatac
     2461 ccttcaaacg aaatctacct tcaccccgta aaagatgggt ttggcagcac acttgcctta
     2521 tatctactca tttttactgc aacaggttga aatctcagca ctgtcagaaa gcgctgatga
     2581 ctaaacagcc ctgagccggg cgatgtaacc atcacacaga atcctgatag cgaaatatgg
     2641 cgtgactcga tacttcactc cgcaatgcat tccttgatga attcgcagga ccgtgataca
     2701 cgggacaggt cactgaatga cgacaatgtc ctggaaatca gcgaaccgcg catctgaagt
     2761 acatttgagc gactgtacca gaacatgaat gaggcgtttg gattaggcga ttattagcag
     2821 ggctaagcat tttactatta ttattttccg gttgagggat atagagctat cgacaacaac
     2881 cggaaaaagt ttacgtctat attgctgaag gtacaggcgt ttccataact atttgctcgc
     2941 gttttttact caagaagaaa atgccaaata gcaacatcag gcagacaata cccgaaattg
     3001 cgaagaaaac tgtttggtag cctgcgtggt caaagagtat cccagtcggc gttgaaagca
     3061 gcacaatccc aagcgaactg gcaatttgaa aaccaatcag aaagatcgtc gacgacaggc
     3121 gcttatcaaa gtttgccacg ctgtatttga agacggatat gacacaaagt ggaacctcaa
     3181 tggcatgtaa caacttcact aatgaaataa tccaggggtt aacgaacagc gcgcaggaaa
     3241 ggatacgcaa cgccataatc acaactccga taagtaatgc attttttggc cctacccgat
     3301 tcacaaagaa aggaataatc gccatgcaca gcgcttcgag taccacctgg aatgagttga
     3361 gataaccata caggcgcgtt cctacatcgt gtgattcgaa taaacctgaa taaaagacag
     3421 gaaaaagttg ttgatcaaaa atgttataga aagaccacgt ccccacaata aatatgacga
     3481 aaacccagaa gtttcgatcc ttgaaaactg cgataaaatc ctcttttttt acccctcccg
     3541 catctgccgc tacgcactgg tgatccttat ctttaaaacg catgttgatc atcataaata
     3601 cagcgccaaa tagcgagacc aaccagaagt tgatatgggg actgatacta aaaaatatgc
     3661 cggcaaagaa cgcgccaata gcatagccaa aagatcccca ggcgcgcgct gttccatatt
     3721 cgaaatgaaa atttcgcgcc attttttcgg tgaagctatc aagcaaaccg catcccgcca
     3781 gataccccaa gccaaaaaat agcgccccca gaattagacc tacagaaaaa ttgctttgca
     3841 gtaacggttc ataaacgtaa atcataaacg gtccggtcaa gaccaggatg aaactcatac
     3901 accagatgag cggtttcttc agaccgagtt tatcctgaac gatgccgtag aacatcataa
     3961 atagaatgct ggtaaactgg ttgaccgaat aaagtgtacc taattccgtc cctgtcaacc
     4021 ctagatgtcc tttcagccaa atagcgtata acgaccacca cagcgaccag gaaataaaaa
     4081 agagaaatga gtaactggat gcaaaacgat agtacgcatt tctgaatgga atattcagtg
     4141 ccataattac ctgcctgtcg ttaaaaaatt cacgtcctat ttagagataa gagcgacttc
     4201 gccgtttact tctcactatt ccagttcttg tcgacatggc agcgctgtca ttgccccttt
     4261 cgccgttact gcaagcgctc cgcaacgttg agcgagatcg ataattcgtc gcatttctct
     4321 ctcatctgta gataatcccg tagaggacag acctgtgagt aacccggcaa cgaacgcatc
     4381 tcccgccccc gtgctatcga cacaattcac agacattcca gcaaaatggt gaacttgtcc
     4441 tcgataacag accaccacac cttctgcacc tttagtcacc aacagcatgg cgatctcata
     4501 ctcttttgcc agggcgcata tatcccgatc gttctgtgtt tttccactga taagtcgcca
     4561 ttcttcttcc gagagcttga cgacatccgc cagttgtagc gcctgccgca aacacaagcg
     4621 gagcaaatgc tcgtcttgcc atagatcttc acgaatatta ggatcgaagc tgacaaaacc
     4681 tccggcatgc cggatcgccg tcatcgcagt aaatgcgctg gtacgcgaag gctcggcaga
     4741 caacgcaatt gaacagagat gtaaccattc gccatgtcgc cagcagggca agtctgtcgt
     4801 ctctaaaaaa agatcggcac tggggcggac cataaacgta aatgaacgtt ccccttgatc
     4861 gttcagatcg acaagcaccg tggatgtccg gtgccattca tcttgcttca gatacgtgat
     4921 atcgactccc tcagttagca gcgttctttg cattaacgca ccaaaaggat catcccccac
     4981 ccgacctata aacccacttg ttccgcctaa tctggcgatt cccaccgcaa cgttagctgg
     5041 cgcgccgcca ggacaaggca gtaggcgccc gtctgattct ggcaagagat ctacgaccgc
     5101 atcccctaaa acccatactt tggctgacat ttttttccct taaattcatc tgagttacgc
     5161 atagtgataa acctcttttt cgcaaaatcg tcatggattt actaaaacat gcatattcga
     5221 tcacaaaacg tcatagttaa cgttaacatt tgtgatattc atcgcattta tgaaagtaag
     5281 ggactttatt tttataaaag ttaacgttaa caattcacca aatttgctta accaggatga
     5341 ttaaaatgac gcaatctcga ttgcatgcgg cgcaaaacgc cctagcaaaa cttcatgagc
     5401 accggggtaa cactttctat ccccattttc acctcgcgcc tcctgccggg tggatgaacg
     5461 atccaaacgg cctgatctgg tttaacgatc gttatcacgc gttttatcaa catcatccga
     5521 tgagcgaaca ctgggggcca atgcactggg gacatgccac cagcgacgat atgatccact
     5581 ggcagcatga gcctattgcg ctagcgccag gagacgataa tgacaaagac gggtgttttt
     5641 caggtagtgc tgtcgatgac aatggtgtcc tctcacttat ctacaccgga cacgtctggc
     5701 tcgatggtgc aggtaatgac gatgcaattc gcgaagtaca atgtctggct accagtcggg
     5761 atggtattca tttcgagaaa cagggtgtga tcctcactcc accagaagga atcatgcact
     5821 tccgcgatcc taaagtgtgg cgtgaagccg acacatggtg gatggtagtc ggggcgaaag
     5881 atccaggcaa cacggggcag atcctgcttt atcgcggcag ttcattgcgt gaatggacct
     5941 tcgatcgcgt actggcccac gctgatgcgg gtgaaagcta tatgtgggaa tgtccggact
     6001 ttttcagcct tggcgatcag cattatctga tgttttcccc gcagggaatg aatgccgagg
     6061 gatacagtta ccgaaatcgc tttcaaagtg gcgtaatacc cggaatgtgg tcgccaggac
     6121 gactttttgc acaatccggg cattttactg aacttgataa cgggcatgac ttttatgcac
     6181 cacaaagctt tttagcgaag gatggtcggc gtattgttat cggctggatg gatatgtggg
     6241 aatcgccaat gccctcaaaa cgtgaaggat gggcaggctg catgacgctg gcgcgcgagc
     6301 tatcagagag caatggcaaa cttctacaac gcccggttca cgaagctgag tcgttacgcc
     6361 agcagcatca atctgtctct ccccgcacaa tcagcaataa atatgttttg caggaaaacg
     6421 cgcaagcagt tgagattcag ttgcagtggg cgctgaagaa cagtgatgcc gaacattacg
     6481 gattacagct cggcactgga atgcggctgt atattgataa ccaatctgag cgacttgttt
     6541 tgtggcggta ttacccacac gagaatttag acggctaccg tagtattccc ctcccgcagc
     6601 gtgacacgct cgccctaagg atatttatcg atacatcatc cgtggaagta tttattaacg
     6661 acggggaagc ggtgatgagt agtcgaatct atccgcagcc agaagaacgg gaactgtcgc
     6721 tttatgcctc ccacggagtg gctgtgctgc aacatggagc actctggcta ctgggttaac
     6781 ataatatcag gtggaacaac ggatcaacag cgggcaaggg atccgcgtca ctcttccccc
     6841 ttcacgacct tcaataatat gcaatgcagc ttcccgcccg ataatgtcat gtggaagctg
     6901 aattgtggtc agcggcggta aaaacagatg cccgacgcca accagattat caaagcccat
     6961 tacggcgaca tcctgcggga tacgtacccc cttcgccagg agaacctgat aagcgacaaa
     7021 ggctgcgcga tcgttaccac atatcagaac atcaaaatct ggtttgcccg gtttgaagtg
     7081 ggcattgagt aaacttgcga gatcggtgta gtgatcatca cctgttgcca tgtgaaattg
     7141 tttcacctca gccagatctc gtccagcatc acgccaggcc tgctcaaatc cctgccgacg
     7201 ataccctgtt gccaacgcac tttccggtag ccagaagcat aacggttgac gatagcccgc
     7261 cgcgagcaaa tgctgtgttg attcatattg tgcagtgtaa tcatcaggga tataactggg
     7321 taacgctggg tcatccgcca cacagttcgc caatacaata ttttcaccat acagagactc
     7381 aggcagcgtg atatgtcgca gccccattgt agtatagata atgccatccg gacggtgggc
     7441 aagcagctga cgtgccgcgc gggcagcgtc atcttcagaa aaaatattga ttaaaaaact
     7501 attccagccg aactcgctgg cggtttgctc aatggcaagc agaatatcaa cagagaaagg
     7561 agtggtagcc gtgtcctgcg ccagcacggc gagagtcgac ggcttacgtc cttgagcgcg
     7621 catcttacgg gcggaaagat caggaacata attcagggtc tggattgcct gcaatacgcg
     7681 gtcacgcgtt gcaggacgca cagattctgc attatgcatc acccgggaga ctgtcatcat
     7741 cgacactccc gccaggcgtg cgacatcctt taatgaagcc atacccaagc cgtttgccgt
     7801 aaaacgggca ctgtagcaga aacagacgtc actggcgaga tccaacgata ttctcaacgc
     7861 cctatcaccc aacacggcaa tacaataaaa aataacaata attgccggat aattgtactc
     7921 aattccgcct ctgttctcgc caacgagtaa ccatataggc aagtaagtaa aacatgccaa
     7981 ctggcatcgc caacctggag aagaaaatat aaatatggat cattgtgctg ccgctgtatc
     8041 ccgacatcag cccggaaatt attgcgattg ctatcggttc aggtgcaatt ggctgcacga
     8101 tcgttacgga ctcgcttttc tggctagtga agcaatattg cggcgctacg ctcaatgaaa
     8161 catttaaata ctatacgaca gcgacattta tcgcttcagt cgtcgctctg gcgggcacat
     8221 tcctgctgtc atttatcatc taagcgcaaa gagacgtact atggaaaacg ctaaaatgaa
     8281 ctcgctcatc gcccagtatc cgttggtaaa ggatctggtt gctcttaaag aaaccacctg
     8341 gtttaatcct ggcacgacct cattggctga aggtttacct tatgttggcc tgaccgaaca
     8401 ggatgttcag gacgcccatg cgcgcttatc ccgttttgca ccctatctgg caaaagcatt
     8461 tcctgaaact gctgccactg gggggattat tgaatcagaa ctggttgcca ttccagctat
     8521 gcaaaaacgg ctggaaaaag aatatcagca accgatcagc gggcaactgt tactgaaaaa
     8581 agatagccat ttgcccattt ccggctccat aaaagcacgc ggcgggattt atgaagtcct
     8641 ggcacacgca gaaaaactgg ctctggaagc ggggttgctg acgcttgatg atgactacag
     8701 caaactgctt tctccggagt ttaaacagtt ctttagccaa tacagcattg ctgtgggctc
     8761 aaccggaaat ctggggttat caatcggcat tatgagcgcc cgcattggct ttaaggtgac
     8821 agttcatatg tctgctgatg cccgggcatg gaaaaaagcg aaactgcgca gccatggcgt
     8881 tacggtcgtg gaatatgagc aagattatgg tgttgccgtc gaggaaggac gtaaagcagc
     8941 gcagtctgac ccgaactgtt tctttattga tgacgaaaat tcccgcacgt tgttccttgg
     9001 gtattccgtc gctggccagc gtcttaaagc gcaatttgcc cagcaaggcc gtatcgtcga
     9061 tgctgataac cctctgtttg tctatctgcc gtgtggtgtt ggcggtggtc ctggtggcgt
     9121 cgcattcggg cttaaactgg cgtttggcga tcatgttcac tgcttttttg ccgaaccaac
     9181 gcactcccct tgtatgttgt taggcgtcca tacaggatta cacgatcaga tttctgttca
     9241 ggatattggt atcgacaacc ttaccgcagc ggatggcctt gcagttggtc gcgcatcagg
     9301 ctttgtcggg cgggcaatgg agcgtctgct ggatggcttc tataccctta gcgatcaaac
     9361 catgtatgac atgcttggct ggctggcgca ggaagaaggt attcgtcttg aaccttcggc
     9421 actggcgggt atggccggac ctcagcgcgt gtgtgcatca gtaagttacc aacagatgca
     9481 cggtttcagc gcagaacaac tgcgtaatac cactcatctg gtgtgggcga cgggaggtgg
     9541 aatggtgccg gaagaagaga tgaatcaata tctggcaaaa ggccgttaat aacgtttcaa
     9601 cgcagcatcg caatcctttc cctgggtgag cgatgctgcc gatggcgcag acttaagatc
     9661 cccggtctta cccgctataa ccccctcctt ttatcatcac ccaacgcctt tcgctgtaaa
     9721 cggcggtttc gcaaaccaaa ccaaaaccgt aagtaagata aaagcaatag ccgccatacg
     9781 gaaaatctca tttgcagaaa tagaaagtga ctgctgggtt atttcattat taatttcatt
     9841 aagaactcct gaaagcgaac catagtattt atccataatt tgtgacgatg aattaaacac
     9901 ggggttaaat tgatcgatgg ttgctgtcaa ctgactatgg tgtaacgatt cgcgtcgtcc
     9961 ccacagcgtc attgtcaacg acgtaccaac tgatcctgac aaggtacgaa aaaaattact
    10021 catactcgag gcattggcaa atttattatc tggcaagcct gaaaacgaaa tcgttgttaa
    10081 gggtaaaaag aaacaggcaa cggcgaatcc ctggaaaaac tgtggcaaaa tgatgcctgt
    10141 aaaatcaatc gttggcataa atgtcacaga acgccagtaa tagcaaaccg catacatcaa
    10201 aaaactgaat gtcactaaca cccgcatgtc tattttattg ccataacgtc ctatcaaagg
    10261 tgaaatcaat agtggcatga tgccgatgtg cgcataagca agcccggccc atatcgcatt
    10321 ataccccatc gtttcctgga gtaactgcgg cataaggacg atcgctccag agtaaaataa
    10381 atacgcgcag gtgatactca caataccaat ggtgaaatta cgagacttaa acaaactgag
    10441 atcaagaatc ggattctctg aggtcgactc ccaaatgact aaagagatca gagaaataac
    10501 tgatactact gttaatatta ttattgtact cgagttgaac caatccagat cgcgcccttt
    10561 atcaagcata atttgcaagc caccaacacc gagcactaac agggtcagtc ctggtagatt
    10621 catttttacc ggtgaagttt cagtttctct tcctttaagt aaggttaagc ataatgtcag
    10681 gacgataatc cccataggga cattgattaa aaatatccaa ccccagctaa agttatcgca
    10741 aatataaccg cccaatatcg gcccacatat cggagcgata atcacggtca ttgaccataa
    10801 tgccagagca aatgttcttt tttctggtgg ataattcctc aataataaac tctgtgacag
    10861 tggaattaac ggccccgcca ttaatccctg aacgactcta aaaaatatca gcacatcaag
    10921 attggtcgat aaactacaca ttaaggaaga cagcgaaaaa aaagtgactg aaagtaaaaa
    10981 taaccttaat tcgcctattc tttgtgccag cctgccggta acagggatcg caatggcatt
    11041 tgctacgcca aacgaggtga taacccaggt gccttcgtct gttgatgctc ccagaaaacc
    11101 agatattgtc ggtattgcga cgttagaaat agtggaatcc aacatttgca taaatgtcgc
    11161 taatgacaat gcaatagtga cgcaccataa cgtcccaccg gttaatggtg ccggagttga
    11221 tttagtgatt gccattaaag ttgtccatta tgcgaaataa tgttgctaat ttctttttct
    11281 atcggactgg tatcaataac taaagcctta ctggtataag ccggcatgga ggtcacggtt
    11341 gaagccagct caggcatctc ggcaatgtct tcgttcttcg tatcaatagt tgctgtcatc
    11401 gataaaccaa tacgcaaggg atgttccatg agttcttttg gatcaagaga aacttcaacc
    11461 ggtacacgct gaacgatttt gatccagttc cctgtcgcat tttgtgcagg taataaggag
    11521 aacgcattgc cggttcccat attgatccct gtcacccgac catgaaacac aacattttca
    11581 ccataaagat cgctgataat attgaccgat tgaccaatcc gtacatccgt aagttgtgtt
    11641 tctttaaagt tggcattaac ccacatttga cgtgccggta cgacagccat taacgattgt
    11701 ccggggctca ctgtttcgcc gacctgaaca cttctctggg caatatagcc ggtaaccgga
    11761 ctcttaatat ccgtacgttt aagcgccaac caggcttctt tagttgcatc cgccgcttca
    11821 acgacttgtg gctgacggtt taatggtgtg ttcattatta aagctttatt cgctttataa
    11881 gcctggatag cggcattcaa tgccgctttg ctacttatta acgtatcttt ggtatgctcc
    11941 agcgtttctt ttgaaataac cccctgcttc gctaacggca ctcgacggtt ataatcttct
    12001 aaagattgtt gatactgaat acgtgctgaa gcgacttcgg cactgtattg tttatcctgt
    12061 aagtatagtt tattcgtttg ccgaacaata tttgccagat tatttttagc tttattgagt
    12121 gcgatagtgg catcagtttt atccagtgaa actaaaatgt caccttgtcg aacgtagttc
    12181 gtatctttat gattaacgac agtgacacta cctgagactt gtgcagaaat tggatctgca
    12241 ttccccgtga cataggcgtc atctgtacta atcatgtctt ctaattccat tgaccaatag
    12301 gcataggcac ctgaaaacgc aataaataaa actaccgcca
//
LOCUS       DAZGVQ010000147        12286 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000147 DAZGVQ010000000
VERSION     DAZGVQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.148"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1171)
                     /gene="ydcO"
                     /locus_tag="ACHLAG_004063"
     CDS             complement(<1..1171)
                     /gene="ydcO"
                     /locus_tag="ACHLAG_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFP0657198.1"
                     /translation="MRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGATTAQIS
                     GWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGVFIVTNA
                     LIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSMLLVWLA
                     TKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPDFSFAHSLSVALPL
                     FLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAITAAICQ
                     SPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQMLAGLALLSTISG
                     SLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNR"
     gene            1263..1799
                     /gene="sutR"
                     /locus_tag="ACHLAG_004064"
     CDS             1263..1799
                     /gene="sutR"
                     /locus_tag="ACHLAG_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFP0657199.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1872..3833
                     /gene="rlhA"
                     /locus_tag="ACHLAG_004065"
     CDS             1872..3833
                     /gene="rlhA"
                     /locus_tag="ACHLAG_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFP0657200.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLFKRKG"
     gene            complement(3925..4155)
                     /gene="yncJ"
                     /locus_tag="ACHLAG_004066"
     CDS             complement(3925..4155)
                     /gene="yncJ"
                     /locus_tag="ACHLAG_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFP0657201.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4377..4553
                     /gene="hicA"
                     /locus_tag="ACHLAG_004067"
     CDS             4377..4553
                     /gene="hicA"
                     /locus_tag="ACHLAG_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFP0657202.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            4599..5015
                     /gene="hicB"
                     /locus_tag="ACHLAG_004068"
     CDS             4599..5015
                     /gene="hicB"
                     /locus_tag="ACHLAG_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFP0657203.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVEIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5094..6500
                     /gene="ydcR"
                     /locus_tag="ACHLAG_004069"
     CDS             5094..6500
                     /gene="ydcR"
                     /locus_tag="ACHLAG_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP0657204.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTIS
                     HAYQLLESLGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKFLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            6745..7890
                     /gene="ydcS"
                     /locus_tag="ACHLAG_004070"
     CDS             6745..7890
                     /gene="ydcS"
                     /locus_tag="ACHLAG_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFP0657205.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            7908..8921
                     /gene="ydcT"
                     /locus_tag="ACHLAG_004071"
     CDS             7908..8921
                     /gene="ydcT"
                     /locus_tag="ACHLAG_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP0657206.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            8922..9863
                     /gene="ydcU"
                     /locus_tag="ACHLAG_004072"
     CDS             8922..9863
                     /gene="ydcU"
                     /locus_tag="ACHLAG_004072"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0657207.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            9853..10647
                     /gene="ydcV"
                     /locus_tag="ACHLAG_004073"
     CDS             9853..10647
                     /gene="ydcV"
                     /locus_tag="ACHLAG_004073"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP0657208.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDSVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRARDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10669..12093
                     /gene="patD"
                     /locus_tag="ACHLAG_004074"
     CDS             10669..12093
                     /gene="patD"
                     /locus_tag="ACHLAG_004074"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFP0657209.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         2974 a         3002 c         3281 g         3029 t
ORIGIN      
        1 atcggtttct gtcagcgatt aaattcaaga ccacgtaaca aacgcctccg gcaattaatc
       61 cccagaacgc agaaccaatc ccgaccagcg tcaatccact tgccgttacc agaaatgcca
      121 ccaccgccgc gtcgcgctca cgctcattat gcagcgcctg atacaaactg ccgctaatgg
      181 tacttaacag cgccagacct gccagcatct ggatccaact tacgggcagg gcagccatca
      241 tcccggtaat agcactacca aacagacctg cgatcaaata aaaaatgcct gcaacggcag
      301 ctgccagcca acgttgatct ttatccggat gcgcttccgg gctttggcaa atagccgcgg
      361 tgattgccgc aataccgacg gaataaacgc cgaaagggga aaaaaccagt gccagcaatc
      421 cagtaaatac aattaatggc gaaacaggag ccgaataccc ggctgctttc attgctgcga
      481 tacccggtgc gttttgcgat gccatcgtca ccagaaaaag ggggagtgca acgctcaggc
      541 tgtgagcaaa cgaaaaatca ggggtaatat aagtggggag aacgggttta aagacaacat
      601 cagttgtgac aacgtcacct tgcgcgataa cgatcacgat cccaataatc atcgcggcaa
      661 ttaccgcata gcgcggcgca acggccttgg ttgccagcca taccagcaac atacttccac
      721 acaacgtaaa ttgaccgtcc agactggcaa acgcctgtaa accaaagcgt aataaaatcc
      781 cggcaagcat tgccgccgca agcgagtgcg gaataatgcg catcagacga gcaaagagtc
      841 ccgttatgcc gcagaggact attaacgcgt tggtgacaat aaaaacgccg atggcttcgt
      901 taagtgttag tccctgcaat ccggtgacca acaaagccgc gccaggcgtt gaccatgcgg
      961 tgagaacagg tacgcgatac cataatgtca gagtcagcgt actgacgccc attgccagcc
     1021 ccagcgccgt catccagcca gagatttgtg cagtggtggc tccggcgaca atcgctgctt
     1081 gccagattat tgccgctgaa ctggcgtagc caattaatac cgccagaaac cccgccagta
     1141 gcgtgggtgg aggaatagaa aacagacgca tagtcacctc gtgcgttata gcgtacaggc
     1201 accgtagcat ttgtccgtta taacgcacaa gtgataaact tccgttttgc cggaggagtc
     1261 gcatggaaaa tctcgctcgc tttttatcca ccacacttaa acaactacgc cagcagcgcg
     1321 gctggagtct ttcgcgactg gcagaagcga cgggcgtttc taaagcaatg ttagggcaga
     1381 ttgagcgtaa tgagtccagt ccgacggtcg cgacattatg gaaaattgcc accgggttga
     1441 acgtaccatt ttcaacattt atttctccgc cgcagtccgc tacgccttca gtttatgatc
     1501 cacaacagca ggcgatggtg atcacttcgc tgtttcctta tgatccacaa ctctgctttg
     1561 aacacttctc gattcagatg gcatctggtg caataagcga atcgacgccc catgagaagg
     1621 gggtaatcga acatgtcgtc gtcattgatg gacaacttga tctgtgcgtt gatggggaat
     1681 ggcagaccct taattgtggg gaaggcgttc gatttgctgc agacgtcacg catatctatc
     1741 gtaatggtgg ggagcaaacc gtacattttc attccctcat ccattacccg cgcagttaag
     1801 cgggaaaact atttcgcaac gtcgcgcttg tggctaaaat agccgccatt tttcagctac
     1861 tggataagaa tgtgaccgta tcttctcatc gacttgaact gttaagcccg gcacgcgatg
     1921 ccgccattgc ccgcgaagct attttgcacg gtgccgatgc tgtttatatc ggcggccctg
     1981 gttttggtgc ccgtcataat gccagtaata gcctgaaaga tattgccgag ctggtgccgt
     2041 ttgcccatcg ttatggtgca aaaattttcg tcacgcttaa caccattttg catgatgatg
     2101 agctggaacc cgcgcaacgg ctgattactg acctctacca gaccggtgtc gatgcgctga
     2161 ttgttcagga tatggggatt ctggaacttg atattccgcc gattgaactg cacgccagta
     2221 cgcagtgcga cattcgtaca gttgaaaaag cgaagttcct ctctgatgtt ggcttcacgc
     2281 agattgtgct ggcgcgagag ctgaatcttg atcagatccg cgcgattcac caggctacgg
     2341 acgcgaccat tgaattcttt attcatgggg cactgtgcgt ggcctattcg ggtcagtgct
     2401 acatttctca tgcgcaaaca gggcgtagcg ccaaccgtgg cgattgctcg caggcgtgcc
     2461 gtttgccata cacattgaaa gacgatcagg ggcgggtggt ttcctatgaa aaacatctgc
     2521 tgtcgatgaa agataacgat cagactgcca acctcggcgc gctgattgat gctggtgtac
     2581 gctccttcaa gattgaaggg cgttacaaag atatgagcta cgtgaagaat atcaccgccc
     2641 attatcgcca gatgcttgat gccattattg aagaacgtgg cgatctggcg cgcgcttcat
     2701 caggtcgtac tgaacatttc tttgttccat cgacggaaaa gactttccac cgtggtagca
     2761 cagattattt tgtgaatgcc cgtaaaggcg atattggcgc gttcgattcg ccgaaattta
     2821 tcggcctgcc ggtaggcgaa gtattgaaag tggcgaaaga tcatctcgat gttgccgtta
     2881 ccgagccact ggcaaatggc gatggcctga acgtgttgat taaacgtgaa gtcgtcggtt
     2941 ttcgtgccaa tacggtcgag aaaaccggag aaaatcagta ccgcgtctgg cccaatgaaa
     3001 tgccagcaga tttgcacaaa attcgtccac atcacccact aaaccgtaat cttgatcata
     3061 actggcagca ggcactgaca aaaacctcca gcgaacgtcg ggtggcggta gacattgaac
     3121 tgggcggctg gcaggaacaa ctgattctga ccctcaccag tgaagagggt gtcagcatca
     3181 cgcatacgct ggacgggcag ttcgacgaag ccaataacgc cgaaaaagca atgaacaatc
     3241 tgaaggatgg tctggcaaaa ctggggcaaa ccctctatta cgcccgcgat gtgcaaatta
     3301 atttgccggg ggcgctgttt gtaccaaaca gtctgttaaa ccagttccgc cgtgaagctg
     3361 ctgacatgct ggatgctgcg cgtcttgcca gttaccagcg cggcagccgt aaaccggttg
     3421 ctgatcctgc gccggtttat ccgcaaacgc atctgagttt cctcgcgaac gtatacaacc
     3481 agaaagcgcg tgaattttat catcgctatg gtgtgcagct gattgacgcg gcgtatgaag
     3541 cacatgaaga gaagggcgaa gtcccggtga tgatcaccaa gcattgtctg cgctttgcct
     3601 ttaatctgtg cccgaaacag gcgaaaggca atatcaaaag ctggaaggcg acgccaatgc
     3661 aactggttaa cggcgatgaa gtattaacgc taaagtttga ttgccgccca tgcgagatgc
     3721 acgtcattgg caaaatcaaa aatcacatac tgaaaatgcc gttaccggga agcgtagtgg
     3781 catccgtaag tccggatgag ctgctgaaaa cattgtttaa gcgaaaaggg taaaacgcca
     3841 gttttctggt tactcacaac tcattgaatc tgcatgatat tgcctgccgg gtaaggcgtc
     3901 tcgccgcatc cggcaacaaa tgactcaacg ttgtcccgct tctggtttgc gcgatttttg
     3961 ccagtaaaaa tgctcgcgca aaccttccgc cgactcttcc gccacagcac gcaattcatc
     4021 gctgtccgct tcatgacgca gctgatgatc cacattcttt acccacacaa attcatgtcc
     4081 tttgtgccct gccatgagtt gtcctgagaa cagcgcacac gttaataaga caaccgataa
     4141 cgccttcgta aacatcctgc cacctttttc ttaccttttg ccgctatgat gccgatcgtt
     4201 tcttgaggtt attattcagt tttgcaaatt agcgcaaaga aattctggaa tcttccttcc
     4261 tgattttgca tggcattctg ccgttgcggc gatttagtgc tattttcgag caaattacac
     4321 acggaggtaa acaataatgt ttactttgtt gatatactca gcggcaggga ggcgatgtga
     4381 aacaaagcga gttcagacgt tggctcgaat ctcagggcgt cgatgtagcg aatggcagca
     4441 accatttgaa actcaggttt catgggaggc gcagtgtcat gccgcgtcac ccctgcgatg
     4501 agattaaaga accattgcgt aaagcaatcc tgaaacaact cggtttgagt taatcgccaa
     4561 ttaaaaaggt taatgacatg cgagggacag tcgaaattat gcgttatccc gtcactctta
     4621 cacccgcgcc ggaaggcggt tatatggttt cttttgtgga aatccctgaa gcgttgacgc
     4681 agggcgaaac tgtcgctgaa gcgatggaag cggcaaaaga tgctttactg accgcatttg
     4741 atttttattt tgaagataac gagcttatcc ctttaccttc gccattaaat agtcatgatc
     4801 actttattga agtacctttg agcgtcgcct ctaaggtatt gctgttaaat gcttttttac
     4861 agtcagaaat cactcagcaa gagttagcca ggcgaattgg caaacctaaa caggagatta
     4921 ctcgcctatt taacttgcat catgcgacaa aaatcgacgc cgtccagctc gcggcaaagg
     4981 cgcttggcaa agagttatcg ctggtgatgg tttaattaca gttaacgaaa agttgtcatt
     5041 tttaacaact gatatagact gccgaatcat ctgcacataa ttacgattcg ataatgaaaa
     5101 aataccagca gcttgcagaa caattacgcg agcagattgc gtcgggtatc tggcaacccg
     5161 gcgatcgttt gccttcgttg cgtgaccagg tggcgctttc tggcatgagc tttatgacta
     5221 tcagccatgc ctatcagttg ctcgaaagtc tgggatatat tatcgcgcga ccgcagtcgg
     5281 gttattacgt tgcgccacag gcaataaaaa tgccgaaagc gccagtcatt ccagtcactc
     5341 gagatgaagc agtcgatatc aacacttata tttttgatat gttgcaggcc agtcgcgatc
     5401 cgtcggtcgt tccgtttgcc tcggcctttc ccgacccgcg acttttcccc ctccaacaac
     5461 taaaccgctc gctggcgcag gtaagcaaaa ccgccacagc gatgagcgtg attgaaaact
     5521 taccgccagg aaacgcagaa ctgcgtcagg ctattgctcg tcgctatgcc ttacagggca
     5581 tcaccatttc tcctgatgaa attgtcatta ctgccggggc gttagaggca ttaaacctca
     5641 gtttgcaagc ggtaactgaa ccgggcgatt gggtgatagt agagaatcct tgtttctacg
     5701 gtgcgttgca ggcgctggag cggctacggc tgaaggcgtt atcggtggcg acggatgtta
     5761 aagaagggat cgatcttcag gcgctggaac tggcgttgca ggattatccg gtgaaagcgt
     5821 gctggctgat gactaatagc cagaacccac tcggatttac cttaacgccg caaaaaaaag
     5881 cacaactggt ggcgttactc aatcagtaca acgtaacgct gattgaagat gacgtttaca
     5941 gcgaacttta ttttggacgg gaaaaaccgc tgcctgcgaa agcgtgggat cgccacgatg
     6001 gcgttttgca ttgctcttcg ttttcgaaat ttctggtgcc tggttttcgt attggttggg
     6061 tcgccgccgg aaaacatgca cgtaaaattc aacgcttgca gttgatgagt acgctttcca
     6121 ccagctcacc gatgcaactt gcgctggtgg attacctttc cacgcgccga tacgacgccc
     6181 atcttcgtcg cctgcgtcgc cagcttgcgg aacgtaaaca acgtgcctgg caggcactgc
     6241 tgcgttatct gcctgcggaa gttaaaattc atcatagtga cagcggttat tttctctggc
     6301 tggagctccc cgagccgtta gatgccggcg aattaagcct ggcggcactg acgcatcata
     6361 tcagtattgc gccgggtaaa atgttttcta ccggtgaaaa ctggtcacgt ttttttcgtt
     6421 ttaataccgc gtggcagtgg ggagaacgtg aagaacaggc ggtaaaacaa ttaggcaaac
     6481 ttattcaaga acggctgtaa tagcgtttaa tttaattcct cttagattgg gtaatatgaa
     6541 tttcgaatag cagtcatatt tcctaacccc ttgtctatac tccagaagat aaccttacag
     6601 acggcataat gcgcggtagc tcacaacctg attaaatttt ctcaggggcg aaggtgtgcc
     6661 tgcaagccgc cgtctatggt taaacaagga gatattttta cggcacggcg gcagaacaat
     6721 taattacgac aggagtaaga ccttatgagc aagacatttg cccgcagcag cctgtgtgcg
     6781 ctcaccatga caataatgac cgctcacgcc gccgaaccac ctaccaattt agataaaccg
     6841 gaagggcgac tggatattat cgcctggccg ggatacatcg aacgcggaca aactgataaa
     6901 caatacgact gggtaacgca atttgaaaaa gagacaggct gcgcggtgaa tgtgaaaacc
     6961 gccgcgactt ccgatgaaat ggtcagtctg atgaccaaag ggggttacga tctggttacg
     7021 gcatccggcg atgcctcgct gcgtttgatt atgggcaaac gcgtgcagcc gattaatacc
     7081 gccttgattc ccaactggaa aacgctcgat ccgcgcgtgg ttaaaggcga ctggtttaat
     7141 gttggcggca aagtttacgg cacaccttac caatgggggc cgaacctgct gatgtacaac
     7201 actaaaacct tcccgacgcc gccggatagc tggcaagtgg tttttgttga gcaaaatctg
     7261 ccggacggca agagcaataa aggccgcgtt caggcttatg atggccctat ctacattgcg
     7321 gacgctgcgt tgttcgttaa agccactcag ccgcagttgg gcatcagcga tccgtatcaa
     7381 ctaaccgaag aacagtacca ggcggtgctg aaagtgctgc gcgctcaaca cagtttgatc
     7441 catcgctact ggcatgacac taccgtgcaa atgagcgatt tcaaaaacga gggtgtggtt
     7501 gcttccagtg cctggcccta tcaggccaac gccctgaaag ccgaaggcca gcctgttgct
     7561 accgttttcc cgaaggaggg tgttaccggt tgggctgata ccaccatgct gcatagcgaa
     7621 gcgaaacatc cggtttgcgc ctacaaatgg atgaactggt cattaacgcc aaaagtgcag
     7681 ggcgatgtgg cggcctggtt tggctcgtta ccggtagtgc cggaagggtg taaagccagt
     7741 ccgttattag gcgaaaaagg ttgtgaaacc aacggtttta actatttcga caaaatcgcc
     7801 ttctggaaaa cgcctatagc agaagggggc aagtttgttc cctacagtcg ctggacgcag
     7861 gattacattg ccattatggg cggtcgctaa cttcgctggg gtgctttatg acgtacgcag
     7921 tggagtttga caacgtctcg cggttgtacg gtgacgtgcg cgcagtagat ggcgtcagta
     7981 ttgcgataaa agatggtgag ttcttctcta tgctggggcc gtccggctcc ggcaaaacca
     8041 cctgcctgcg cctgattgct ggcttcgaac agctttccgg cggggctatc tctatctttg
     8101 gtaaacccgc cagcaatctg ccaccgtggg agcgggacgt gaatactgtc tttcaggact
     8161 acgcgctatt tccgcatatg tcgattcttg acaatgtcgc ctatgggctg atggtcaaag
     8221 gcgtgaataa aaagcagcgg cacgcaatgg cgcaagaggc gctggagaaa gtggcgttgg
     8281 ggtttgtaca tcaacgtaaa ccgtcacaac tttctggtgg tcagcgccag cgggttgcta
     8341 tcgccagagc attggtgaat gaaccgcgcg tattgctgtt ggatgaaccg ctcggcgcac
     8401 tggatctcaa attgcgtgag cagatgcagc tggaactgaa aaaactgcaa cagtctctcg
     8461 gtatcacttt tatcttcgtt acccacgatc agggcgaagc gttatcgatg tccgatcgtg
     8521 tggcggtttt caataatgga cgcattgagc aggtcgattc cccgcgcgat ctctatatgc
     8581 gcccgcgcac gccgtttgtt gccggattcg tcgggacatc caacgttttt gatggactga
     8641 tggcagagaa actttgtggc atgacgggaa gcttcgccct gcgaccggaa catatccgcc
     8701 tcaacacccc tggtgaactg caggccaatg gcacgatcca ggcggtgcaa tatcagggcg
     8761 cggcaactcg ttttgaactg aaattgaacg gcggtgaaaa actgcttgtg agtcaggcca
     8821 atatgacagg cgaagaactg cctgccacgc tcacgcccgg acaacaggtg atggtttcct
     8881 ggtcgcgtga tgtgatggtg ccgctggttg aggagaggtg aatggcgatg aatgtattgc
     8941 aatcaccttc acgtccaggt ctgggtaagg tgtccggttt cttctggcgt aatccggggc
     9001 tggggctgtt tttactgctg cttggcccgc taatgtggtt tggcattgtc tatttcggct
     9061 cgctgctgac actgttatgg cagggatttt atacttttga cgatttcacc atgtcggtaa
     9121 cgccggaact gacgctggcg aatatccgtg cgctgtttaa tccggcgaat tacgacatca
     9181 ttctccgcac gctgactatg gctgtggcag tcactatcgc cagcgccatt ctggcttttc
     9241 caatggcgtg gtatatggcg cgctatacca gcgggaaaat gaaagctttt ttttatattg
     9301 cggtaatgtt gccgatgtgg gcgagctaca ttgttaaagc ctatgcctgg acgttattgc
     9361 tggcaaaaga tggcgtggct cagtggtttt tacaacatct tgggctggaa ccactgctga
     9421 ctgcgttcct tacattacct gcggtgggcg gaaatacgct gtcaacttcc gggctggggc
     9481 gctttctggt gtttctctat atctggttgc cgttcatgat cctgcccgtt caggcggcgc
     9541 ttgagcgttt gccgccgtca ttgttgcagg cgtcggctga tctcggcgca cgtccacgac
     9601 aaacctttcg ctatgtggtg ctgccgctgg caatcccggg tattgccgct ggctctatct
     9661 ttaccttctc actcacactg ggcgatttta tcgtcccgca gctggttggt cctccaggat
     9721 attttatcgg caatatggtt tattcccagc agggggcgat tggcaatatg ccgatggcgg
     9781 cggcattcac cctggtgccg attgttctca tcgcactgta cctggcgttc gtgaaacgtc
     9841 tgggagcgtt cgatgcactc tgaacgcgca ccgtttttcc tcaaactggc ggcctggggc
     9901 ggcgtggttt tcctacattt tcccatcctg attatcgccg cttatgcgtt taacactgaa
     9961 gatgcggcgt ttagttttcc accgcagggc ctgacgctgc gctggtttag cgtggcagca
    10021 cagcgtagtg atattcttga ttccgtgaca ctgtcactta aagtggcggc gctggcgaca
    10081 ttaatggcgc tggtgttagg gacgctggca gctgccgcgc tgtggcgacg agactttttc
    10141 ggcaaaaacg ccatttcgct gttactgctg ctgcccattg cgctgccggg cattgtcact
    10201 ggtctggcgc tattaaccgc ttttaaaacc atcaatctgg agccgggatt tttcaccatc
    10261 gtggtcggtc atgcgacttt ttgcgtagtg gtggtgttta acaatgtcat cgcccgtttt
    10321 cgccgcacct cgtggagtct ggttgaggcg tcaatggatc ttggggccaa tggctggcaa
    10381 accttccgct acgtagtgtt gccgaatctc agttcggcgc tactggcagg aggaatgctg
    10441 gcgtttgcct tgtcgttcga tgaaatcatc gttacgactt ttacggcagg tcatgaacga
    10501 acgttaccgt tgtggttgct caatcagctt gggcgagcgc gtgatgtacc ggtaactaac
    10561 gtggtggcac tgctggttat gttggtaaca accttgccga tcctgggggc ctggtggcta
    10621 acccgcgaag gcgacaatgg tcaataacca ctgatacagg aatatgctat gcaacataag
    10681 ttactgatta acggagaact ggttagcggc gaaggggaaa aacagcctgt ctataatccg
    10741 gcaacggggg acgttttact ggagattgcc gaggcatccg cagagcaggt cgatgctgct
    10801 gtgcgcgcgg cagatgcagc atttgccgaa tgggggcaaa ccacgccgaa agtgcgtgcg
    10861 gaatgtctgc tgaaactggc tgatgttatc gaagaaaatg gtcaggtttt tgccgaactg
    10921 gagtcccgta attgtggcaa accgctgcat agtgcgttca atgatgaaat cccggcgatt
    10981 gtcgatgttt ttcgcttttt cgcgggtgcg gcgcgctgtc tgaatggtct ggcggcaggt
    11041 gaatatcttg aaggtcatac ttcgatgatc cgtcgcgatc cgttgggggt cgtggcttct
    11101 atcgcaccgt ggaattatcc gctgatgatg gcagcgtgga aactggctcc ggcgctggcg
    11161 gcagggaact gcgtagtgct taaaccatca gaaattaccc cgctgaccgc gttgaagttg
    11221 gcagagctgg cgaaagatat cttcccagca ggcgtgatta acgtactgtt tggcagaggc
    11281 aaaacggtgg gtgatccgct gaccggtcat cccaaagtgc ggatggtgtc gctgacgggc
    11341 tctatcgcca ccggcgagca catcatcagc cataccgcgt cgtccattaa gcgtactcat
    11401 atggaacttg gtggcaaagc gccagtgatt gtttttgatg atgcggatat tgaagcagtg
    11461 gtcgaaggtg tacgtacatt tggctattac aatgctggac aggattgtac tgcggcttgt
    11521 cggatctacg cgcaaaaagg catttacgat acgctggtgg aaaaactggg tgctgcggtg
    11581 gcaacgttaa aatctggtgc gccggatgac gagtctacgg agcttggacc tttaagctcg
    11641 ctggcgcatc tcgaacgcgt cagcaaggca gtagaagagg cgaaagcgac agggcacatc
    11701 aaagtgatca ctggcggtga aaagcgcaag ggtaatggct attactatgc gccgacgctg
    11761 ctggctggcg cattacagga cgatgccatc gtgcaaaaag aggtatttgg tccagtagtg
    11821 agtgttacgc ccttcgacaa cgaagaacag gtggtgaact gggcgaatga cagccagtac
    11881 ggacttgcat cttcggtatg gacgaaagat gtgggcaggg cgcatcgcgt cagcgcacgg
    11941 ctgcaatatg gttgtacctg ggtcaatacc catttcatgc tggtaagtga aatgccgcac
    12001 ggtgggcaga aactttctgg ttacggcaag gatatgtcac tttatgggct ggaggattac
    12061 accgtcgtcc gccacgtcat ggttaaacat taagttagat gttagtggct gttataggac
    12121 ggccactatc gttttaggtt ctgagtttaa tggcggttaa gtattagaat gattacatta
    12181 ataactagcg taaaaattag gctaaatgtt tgatggcgat ttttcatcct gtcacagttg
    12241 attagcataa ggctttagag tatcgttatt aaactaataa tttata
//
LOCUS       DAZGVQ010000148        12237 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000148 DAZGVQ010000000
VERSION     DAZGVQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.149"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..834
                     /locus_tag="ACHLAG_004075"
     CDS             <1..834
                     /locus_tag="ACHLAG_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFP0657210.1"
                     /translation="LKLEKTKLTLVTMTMQDHATLKEIKDLAKNITQQYGTPDLCIEA
                     MRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQNLRAPVPMTWAFMMR
                     EAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQERTFSLSIAENLIAK
                     IQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRSAAIIKDAYIAITPDT
                     SILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNISLDEFAKALKSVLAR
                     I"
     gene            complement(1096..3771)
                     /locus_tag="ACHLAG_004076"
     CDS             complement(1096..3771)
                     /locus_tag="ACHLAG_004076"
                     /inference="COORDINATES: protein motif:HMM:NF020698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP0657211.1"
                     /translation="MIADNNDLTTLTATVADTEGNAIANTEVTFTLPEDVRANFTLSD
                     GGKAITDTEGKAKVTLKGIKAGAHTVTASMAGSKSGQLVVNFTADTLTAQVNLNVTED
                     NFIANNIGMTKLQATVTDGNGNPFANEAVTFTLPADVSASFTLGQGGSAITDINGKAE
                     VTLSGTKSGTYPVTVSVINYGVSDTKQVTLIADAGTATLASLTSVYSFVVSTTEGATM
                     TASVTDANGNPVEGIKVNFRGTSVTLSSTSVETDDQGFAEILVTSTEVGLKTVSASLA
                     DKPTEVISRLLNAKADINSATITSLEIPEGQLMVAQDVAVKAHVNDQFGNPILNESVT
                     FSAEPPEHMTISQNIVSTDTHGIAEVSMTPERNGSYMVKASLANGASLEKQLEAIDEK
                     LTLTASSPLIGVYAPTGTTLTATLTSANGTPVEGQVINFSVTPEGATLSGGKVRTNSS
                     GQAPVVLTSNKVGTYTVTASFHNGVTIQTQTTVKVTGNSSTAHVASFIADPSTIAATN
                     SDLSTLKATVEDGSGNLIEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVKGAMTGS
                     VTVSAVTTAGGMQTVDITLVAGPADASQSVLKNNRSSLKGDFTDSAELHLVLHDISGN
                     PIKVSEGMEFVQSGTNVPYMKISAIDYSLNINGDYKATVTGGGEGIATLIPVLNGVHQ
                     AGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGAYYQLNNDNFAPGKTAAD
                     YEFSSSASWVDVDATGKVTFKNVGSNWERITATPKSGGPSYVYEIRVKSWWVNSGDAF
                     MIYSLAENFCSSNGYTLPRADHLNHSRSRGIGSLYSEWGDMGHYTTDAGFQSNMYWSS
                     SPANSSEQYVVSLATGDQSVFEKLGFAYATCYKNL"
     gene            complement(3925..8613)
                     /locus_tag="ACHLAG_004077"
                     /pseudo
     CDS             complement(3925..8613)
                     /locus_tag="ACHLAG_004077"
                     /inference="COORDINATES: protein motif:HMM:NF023351.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(9392..9467)
                     /locus_tag="ACHLAG_004078"
     tRNA            complement(9392..9467)
                     /locus_tag="ACHLAG_004078"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9432..9434),aa:Asn,seq:gtt)
     gene            complement(9568..10365)
                     /gene="mtfA"
                     /locus_tag="ACHLAG_004079"
     CDS             complement(9568..10365)
                     /gene="mtfA"
                     /locus_tag="ACHLAG_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFP0657212.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            10459..10548
                     /locus_tag="ACHLAG_004080"
     tRNA            10459..10548
                     /locus_tag="ACHLAG_004080"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10493..10495,aa:Ser,seq:cga)
     gene            complement(11118..11648)
                     /gene="yodB"
                     /locus_tag="ACHLAG_004081"
     CDS             complement(11118..11648)
                     /gene="yodB"
                     /locus_tag="ACHLAG_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFP0657213.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(11991..>12237)
                     /locus_tag="ACHLAG_004082"
     CDS             complement(11991..>12237)
                     /locus_tag="ACHLAG_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFP0657214.1"
                     /translation="LTYKSGKKGVRYLFECKDPESKAPKYIQFSDHIIAPRKSSHFHI
                     FMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT         3010 a         2842 c         2918 g         3467 t
ORIGIN      
        1 cttaaacttg aaaagactaa attaacatta gtcacaatga ctatgcagga tcacgcaaca
       61 ttaaaagaaa taaaagattt agcaaagaac ataacacagc aatacggtac gccggatctt
      121 tgtatagagg cgatgcgtaa aaagaacctg aaaacgatga tatttatcag tcagttgaaa
      181 gcaaaaacga attttcaggt tgttggttta accatgaagt gctactcccc tttgtgcaag
      241 aacgcgtccc ggatggatca gaatctccgg gctcctgtac ccatgacatg ggcatttatg
      301 atgcgtgagg cgggttttcc agcagtcagg ccaatatatg aattgccact aagtgaggat
      361 gtactcgatg aggtgcgcga ggaaatgcga tcgttaggat cttacattgc gcttaattta
      421 gaaggtagct cgcaggaacg tacattttca ttatcgattg cagaaaatct aatagcaaaa
      481 attcaaagtg aaacagatat accaatagtg atcgttcatg gaccaaaagg ggaagataaa
      541 gccagggtat tagtggattg ttataataat gtctaccgtt tatccttatc accctcgatt
      601 aaacgttcag cagcaatcat aaaagatgct tatatcgcaa taactcctga cacctcaata
      661 ttacatatgg caagtgccta taatactccc gttgttgcaa tttatgctga ttacaaaacg
      721 cgatggcccg caatggccga tgtttcggag tcagtcgtcg ttgggcaaaa aattgacaat
      781 ataagtctgg atgaattcgc aaaggcatta aaaagtgttt tggcgagaat atgagacagt
      841 acttatgcat agtaatgccg atctgttatg aacggcggtg tcatcaatac tctgaggctg
      901 gtcaatcatc acagaaaacc agcctttgga tcaaagctgt actcaccgga tacgatcata
      961 gtcacatcga acgcgattct tgcggtgcag cagttttatt tattcatttt ctgtgagaga
     1021 agggcaagct ctatagaggg aatgtaaaaa agagtgctcc gaatggagca ctctttaaga
     1081 aattataacg gaaaatcaca ggtttttata acatgtcgca taagcaaacc caagcttttc
     1141 aaatacgctt tgatcacctg ttgccaggga aactacgtat tgttcgcttg agtttgcggg
     1201 actagatgac caatacatat ttgattgaaa accagcgtca gtcgtgtaat gccccatatc
     1261 tccccattca ctgtacagtg acccgatgcc tcgggaacga ctatggttta aatgatctgc
     1321 acggggaagt gtgtagccat tgctactgca aaaattttca gcaaggctgt atatcatgaa
     1381 agcatcgccg gagttcaccc accaactctt cacacggatt tcgtatacat agctagggcc
     1441 tcctgatttt ggcgtcgccg taatcctttc ccaattgctg ccgacatttt taaatgtcac
     1501 tttaccggta gcatcaacat cgacccagga ggcagagctt gaaaactcat aatcagccgc
     1561 cgtttttcct ggggcaaagt tgtcattatt caactgataa tacgccccgg tgaacccctg
     1621 cgaagggaat gtagttgtcg gtaggtcagt accattgact gatactgtac cgctcattat
     1681 tttgtcttct gcgcgagtga attgtattgt ggtactcaga ccagcttgat gaacaccatt
     1741 caatacaggg atcagcgttg cgataccctc tccgccgcct gtaacagtgg ctttgtaatc
     1801 accgttgata tttagactgt aatcaattgc gctaattttc atatagggca cgttagtacc
     1861 tgattgcaca aattccatcc cttcagaaac tttgatcgga ttgcctgata tatcgtgcaa
     1921 aacaagatgt agctcagcac tatcggtaaa gtcccctttc agtgaggacc gattgttctt
     1981 aaggacggac tgcgaggcgt ctgccgggcc tgccaccagc gttatatcta ctgtttgcat
     2041 tccaccagcg gtcgtgactg cgcttaccgt gacgctacct gtcatcgctc ctttcacgct
     2101 tgttgtcgcg attccgtttt gatcggtcac cgctgttaat gacgttaatg tggcagagcc
     2161 gctttttaag gcgaagtaca cagtgagacc ttcgatcagg ttaccactgc catcctcaac
     2221 cgttgccttt aaggtactta aatcactgtt ggtggcggcg atagtcgatg gatcagcgat
     2281 aaagctagca acatgtgcgg tgcttgagtt gccagtgact ttcacggttg tctgtgtctg
     2341 tattgttacg ccgttatgga acgatgcagt caccgtatat gtaccgactt tattgctggt
     2401 cagaacgacc ggagcctgac ctgaagagtt agttctcact tttccgccac ttaacgtcgc
     2461 cccttctggc gttacgctaa agttgatgac ctgaccctcc actggagtgc catttgcaga
     2521 ggttagcgtt gccgtcagag tagtgcctgt aggggcatag acaccgataa gcggactgga
     2581 cgccgtgagt gtcagttttt catcaatagc ctccagttgt ttctcaagtg aggctccatt
     2641 cgccagggat gctttcacca tatacgaacc gtttctttcg ggcgtcatgg agacctcggc
     2701 tataccatgc gtatcagtag agacaatatt ttggctgatg gtcatgtgct ctggtggctc
     2761 tgcactgaat gttacagatt cattaagaat cgggttgcca aactggtcgt tgacgtgagc
     2821 tttaactgct acgtcttgtg cgaccattag ctgaccttca ggtatctcca gactggtaat
     2881 cgttgcagaa ttaatatctg cttttgcatt cagtaatcgc gatatgactt cagtaggttt
     2941 atctgccaga gaggctgaaa ctgttttcag tccgacttcg gtgcttgtca caagaatttc
     3001 agcgaaaccc tgatcatccg tttcaacgct ggtgctgctt agcgtgacgg aggttccgcg
     3061 gaaattaact tttatgcctt ctaccgggtt gccgttagcg tcagtgacgc ttgcagtcat
     3121 ggtcgcgccc tcggtcgtgc tgacgacgaa tgagtataca gaggttaagg aggctagtgt
     3181 tgcggtacca gcatcggcaa tcaaagtcac ctgtttcgta tcactgacac cataattgat
     3241 cacgctaact gtcacgggat aggtgccgga tttggtaccg ctcagtgtaa cttcagcctt
     3301 gccgttgata tcagtaatgg cggaaccgcc ttgtccgaga gtgaagcttg cgctcacatc
     3361 tgccggtagc gtgaatgtca ccgcctcatt ggcgaatggg ttgccgtttc catcagtcac
     3421 tgttgcctgc agcttggtca tcccaatgtt attggcgatg aaattgtcct cggtaacgtt
     3481 aagattaacc tgcgcagtga gcgtatccgc agtaaagttc accaccagtt gtccactctt
     3541 actgccagcc atcgatgctg taacagtatg agcgcctgct tttatacctt tcagcgtgac
     3601 tttcgctttg ccttcagtat cagtaatcgc tttaccgcca tcgctcagcg tgaagttcgc
     3661 cctcacatct tccggcagag taaatgttac ttcagtgttg gctatcgcat tgccctctgt
     3721 atcagcgact gttgctgtta gtgtcgtgag atcgttattg tcggcaatca ccggatcctt
     3781 cgaggctgcc agcgtgattt cggcattcgc gacgttcggc acataggtca ctgtttgttg
     3841 catcgaatcg ccattttcga gggttgccgt cacttgataa accccggctt ttgtagcggt
     3901 aaagcttgtg taaaaattgc cgtcgtggtt ggtagtatat atagcgttat tactgggggt
     3961 cacgccttca cttggtgata ctctgagggt tacctcctgc tggggtacac cgttgccgta
     4021 attatccttc acctcaatat acagattggt agtgtcgcct gcggagactg tatcaaaaag
     4081 tgcctgtagc aaggtgagat gtgccgtaga cgcatcagcg ttgacattaa ttgatgtgct
     4141 aagcgtggag ctaccatttt ccagactggc ctcaacggta tccggtctcg ctcctgattc
     4201 tatcgaggag cgggtattgg tataagtgac ggtagcctta ccctgttcgt tcgttacggc
     4261 ttggccgcca ttcgtcattt cggcagagtt tgttctgctg gtgaagttca cagtcacacc
     4321 tttcaccgga aagccattat tatcaacgac tgtggcggtg atgacgctgc cggagctgtt
     4381 ctgcggggta ccggcgatta tgctgtctgg gacaggcgtc aactcgataa tttttgccgc
     4441 cgctgtgtcg ccaataaaat gcacagtttt gttgtcgctg gcaccattat tagccagtga
     4501 tgcagtgacc gtctgctcac caaaggcaac gcctgcgaga gtggcctgcg cgatgccaga
     4561 ctcattggta ttacttactc ccggggtcag ggtgagtcct gaagaggctg agctgaatgt
     4621 taccggaaga ttattcacct cattgtcgaa ctgatcttta accgttgcag ttagcgttgc
     4681 gttatcgacg ccattacctg tgatctcatc ttttgacatc tgcaggacaa cctgagccga
     4741 ggttttgtcc gccacaaacg ttaccggctg cgaatcactg gtattgttat tacccagcgt
     4801 tgcggtaacc gtatgcgtac ccgctttttt acctttcagc gtgacatgcg cttccccatt
     4861 ggcttgggtg atggcaatgc cgttattttc gagggtaaag tttgccgcaa cgtcctgtgg
     4921 catggtgaag ttgaccgtta tccccgccac cggatgattc gacggatctt tcaccatcgc
     4981 cgtaagctgc acgctgtcac tgttattcgt caccacctgt tgagcaggga tgttcagggt
     5041 gacctgcgcg ttgctggcat cgggcgcaat attgactgtc gtcgtatagc cattgccgtt
     5101 aagcagcgtg gcttcgactg tatgaacacc caaaaccgtg tccttaaggg taacttcagc
     5161 aacaccactg tcgttagtat tgcttgtgct ctgactaagt tgagtatctg ccgggttggt
     5221 actgaaggtg accggtagat cttttactac gttatcaaaa ggatctttca ctgttgccgt
     5281 cagagtcgtc tcatccacgc cattcccaat gatttccgct ttcgatgttt tcagaacgac
     5341 aactgcgctg tccttatccg ccacgaatgt gactggttgc gcatcgctgg cattattgtt
     5401 acccaacgtt gcggtaaccg tatgcatgcc cgctttttta cctttgagcg tgacatgcgc
     5461 ttcgccattg gcctgagtga tggcaatacc gttattttca agggtaaagt ttgccgcaac
     5521 gtcctgtggc atggtgaagt tcaccgttat tcccgccacc ggatgattcg acggatcttt
     5581 caccatcgcc gtcagctgta cgctgtcgct gttattcgtc accacctgtt gagccgggat
     5641 gttcagggtg acctgcgcgt tgctggtatc gggcacaata ttgacgattt tcgtatcgct
     5701 tttaccgtta agcagtatgg cttcggctgt atgaacaccc aaaaccgtgc ctttaagggt
     5761 aacttcagca ataccgttct cattagtatt aagcgccttc agactcagtt gcgtgtctgc
     5821 gggggaggtg cggaagacta ctgacagatt tttaaccacg ttatcaaaag gatctttcac
     5881 tgttgctgtc agagttgtct catccacgcc attcccaatg atttccgctt tcgatgtttg
     5941 cagaacgaca accgctctgt ctttatccgc cacaaacgtc attggctgtg tatcgctgac
     6001 attgctgtta gccagacgag ccgtgatcat atgcgtgccc gctaacgtgc cggtcaggga
     6061 ggcgattgca gtcccgttgc tatccgtcat gccttttccg ctgttgctaa tcgagaaccg
     6121 acttgcgacg tcgtttggca cagagaaggt gatttctttg tcttttaatg ggttgccatt
     6181 tttatcctgc aaggttgcag tcatatgtag cggagctgtg ttggtgacgg tgatatcacc
     6241 tgaaggcaca ctgagggtca gggcagcagt actttgatca ccgataaaat tcacctgttg
     6301 attagcctgg gaaccagagc tcacagaggc cgtaacccta taatcaccat ttttcaaact
     6361 ggtcagcgta gctgtggcga tcccgtcgtg gctattcact tctgtttggc tcagtttcgc
     6421 ctctgctgaa ttaacattga aagtgacctt gacgtcattg agcaggttgc cttttgcatc
     6481 ccggactgtc gcggtcattg tggcgctatc attgccgtca gcgaccactt catttttcga
     6541 cttctgcagc tcaacctgcg cggtactcga gtcgccgaca aaactgacga ttaacgtttg
     6601 tttcacgcca ttttcaaggg tgacttcaac cgtgttgtct tcctgcttac tacttttcag
     6661 atcaaaagtc gccagaccat taacatccgt ttttgcggtg ttttgattgt tgaaggaagt
     6721 tgccgatccg cttaataccg caaacgtgac ggtatgatca ttgattgggt tgcttccttc
     6781 atcagcgaca ttgaccgaga cggtgtttgc tgcattctca ttggcgagca caccattatt
     6841 gctggcagat aatgtcgcaa ttttcgctga ctgcgggttg gcgtcgatga taaatccagc
     6901 ggtatgtaaa tcttcattcc agttttgcat taatagcttc gcagtaagcc cactgcctct
     6961 ggtataggcg gtataggtcg ccttatagac gccatctgcg gtttctttcc agtctgtagt
     7021 gactcctggt ttcacgttgt cgatgctgac tgcggtattc agttgctgtt tttgctcctt
     7081 aacaggtctg tcattttcat ctctcagttc taccgtcacc tcgataggat tgccggagag
     7141 ataacggtcc ttatcaatct taattgacga gtgagttcgg gatgacgaaa cagaaatgat
     7201 attcaccacg gcgggggctt tagccgcatc cacaccgttc agctgtggca taagcgtcag
     7261 cgtgccagac atcgctcctg tggtcaggac ttgggtatag cttccgtcac cattatcttt
     7321 ccagtccgac agggtgatgt cctgaacacc ttcgtgacgc gtcgaaagca ccagcccgat
     7381 aacaggatta cctgctgcat catgcgcaat aaaagttagt gtggcggttg aatgggaatc
     7441 cgcgttcaat gtttgggtac ttaatgatac tgaggaatct ttctggctta gcgtaggtgc
     7501 ctgaacgacc accatgctct gttcacgatt cgacaaattg cctttgacat cttcagcggt
     7561 gacttcaatc ggccaggtgt tatcggtttc tggcgtactg gtgaaccggt acgccggcag
     7621 ggtaaccaga atatctttac ccgttgtaac cactttacca ccggcagctt ccagagcggt
     7681 ggcttcgacg ttatagcctt tcagggcata tttggtttgt agcgacgaaa ccagtgattt
     7741 cacttctcct gacttccctg tcacggggtc tgtcagggtc aggcgaacca gttctttttt
     7801 gcgatattcc agaacgatat tgttgttgcg atccaccaga tcataacggc tgcctgcaag
     7861 gctacgccgt gcagcgactt cattcgggtc aagctgtttc tgcattgcgc tgccaggtag
     7921 ccaggtaaaa tcgacggcaa aacgggtgtc attttcgccc tgtttaccct ggcgttgctc
     7981 cgcgctgaag gtcatcagcg ggaagggggt atagttaagt ccagcggtta tggcatgagg
     8041 attactttgc cgatcgtctt tatcgaacag ggccacttca tcgccataat actgttcata
     8101 gaccagttta ccgccaaggt gcggccaggc gggtaaccag ccttctgcgc gtacatccca
     8161 gccattggcc gggcgggctt cataatcgtt gtccagctca ggtgcgctgc gccagttggt
     8221 cagtcgcaaa tagccgttac tgcttaattt tagatagtcg cgccagtact ccgcgctaat
     8281 gccggcgcgg gagtggtaac ggctaagatc gtggtcgaaa aagaagttga tgcccgacat
     8341 ccatgtggga gtgaaatgac gccaacccaa gccgttgtta atctgcgtac gctcgtcagt
     8401 acgatggaga gtatgctgac tgaaaaagag attatcaggc gtttcatacc acggatggag
     8461 aaaatcgaac tgggagttct tcaggctaaa atcttcatcc acgcccagcg tgattcttgc
     8521 ggtaccgaag cggcttaacc agtctgtcat tgcgcctgaa gcctgagaag aggcccatcc
     8581 acgcgccata tttgccgctt gttcgctgtt catatcctcg gcgagcaaag acccgatttg
     8641 ctgtgaagta ctggctatct gttgctcaag gttgccactg ctattacccg gtggcggggt
     8701 taaattattt tcactaactt gtgccgggac atccagttca tcaccctggc ggacattatc
     8761 aaaacctcga gcaaacgtac gaaattggtt gagtttgcgt aactcagcca ccgaaatacc
     8821 gaaacgttcg gcaacgcttt gggccgattc cagcgctcca agggtgtagg gcaccgtatt
     8881 ggcatttgca atggcaatac ggtgcaggaa ctggttgttg ggttgcggcg tttaccacac
     8941 cttgtgctgc cgcagccata gggaacgcaa gttgagttat cagacagata cccgcagtta
     9001 agcggcgtag tttaattccc atcccgcata agatttgtcg gtcatttatt tcttctccac
     9061 ttctcttctt cgtagccatg cttaccttcc ctgaacgagc taacataaat gcgccctttc
     9121 tagcacagcg aaaaaatgta aatcctgata gcaatatgat tgaaaattaa taacttttcg
     9181 ttaggcagtt ttgggtgtga gttgcaagag gggagactac tgaataactc aagttttata
     9241 atcgagggga aaatggtgat ggcgttcata gcaaaacgcc ctcaaccata aaggtcgagg
     9301 gcgcttaaga tgttaaaaac ccgctatccg ttaataaaca atgttcaact aaggttagtg
     9361 acattgcgct aagaagcgaa tttttagaat ttggctcctc tgactggact cgaaccagtg
     9421 acatacggat taacagtccg ccgttctacc gactgaacta cagaggaatc gtgtgaacgg
     9481 ggcgaatatt acttagcggt accttgtctg tcaacactaa atttcataag tcatttcaat
     9541 tggttaatta atctgcaaag ttgttaatta atgaacattc gtcgccgaaa acgagtctgt
     9601 atcattagtg cgatgcagtc tctgcaaagg atcttgttga taaaattggc agaaacgttg
     9661 ccacaatgaa gggaaacgag gagcaaaaag ttctggggcg ctaaagaaat attcagaaag
     9721 tacggcaaaa cattcagcag gatcactggc agcataagca tcaatgctcg ccgcattctc
     9781 gccaaccaat tcgatttctt cctgaatgtt gttcattgca gcatgaagat cgtgttccca
     9841 gccagcaacc tcacgcaacg gaataaaggg aactccgctg gcgcgatcgc cgttacgggt
     9901 gtccagctta tgagcgactt catgaataat caggttaaaa ccagaagcat caaaagaatc
     9961 ttgtatatcc aaccagttca aaacgatagg cccttgctgc cagctctgac ctgactgaac
    10021 aatacgttgg ttatgcacca gaccgatatc gtcttcccat tcatcatcga ccacaaatgg
    10081 cgcaggataa attaagactt catgaaaacc atccagccat tccagtccta actccagaac
    10141 gggtaggcaa aatagaagtg ctatccggca gcttcttaat gaatccagtt caaagccctg
    10201 taaaggaaca agccgctttt gctgtaaaaa acgttcggca agagtgacta atttgctttg
    10261 ttcctgttcc gtcagacacg ttaaaagggg gatcgatagt gcttcctgcc agggaagggc
    10321 agtttgatgt gctgattctt gtactttcca gggccactta atcatcgttt tgctcgcaaa
    10381 ctcgtcactt gaacaaaatt gcacggacag ggactgttaa aatgccaaat ttcctggcat
    10441 catggcaacc atctgaacgg agagatgccg gagcggctga acggaccggt ctcgaaaacc
    10501 ggagtagggg caactctacc gggggttcaa atccccctct ctccgccact ttatcaatga
    10561 cttatctccc gacttcccgc cttgcttttc ctaaacagaa caatcgtaga atattcttga
    10621 agggttagat cgtcactgtt ttctgttcga tactgtgaca ttcagcactt gattcgctat
    10681 ggatctgaca ggaagctttc gagcgaaaat gtgcagttat tcagtcgttt tcttatcgct
    10741 caccattatt cttttagaca ttcatcctat gaaactgccg caaagttggt ggtgggaact
    10801 gaagttgcgt agagaagggg tcaataattg agtcaaaaaa ggaacgcggc cgtcagatta
    10861 aagccgcggg acaaagtcca taatttccaa gagttgttga atttcccagc ttttttgaaa
    10921 gcctttgtat catactaaca agtaaaaaag gtttaaatac aaaaattaac ctgtattttt
    10981 gataagtctt accattgtac gatttacaca gtttctgctc ctccttgttg atagtggaaa
    11041 gggtgcattg tgcacttgcg taaacattta tgacgcgttg atgtctatgg ttcacatttc
    11101 cttgtcaggg gatcgactta tttgcggcga ggcatcattc gtaacagagt attatctttc
    11161 tgaatgtagt gatgaaagag tgctgcgcca gcgtgagctg cgattaaaaa gtaacctata
    11221 tttgcccagg tttcgtgaat attttttatc agtgctttaa tttcgctgtt tggggtaaca
    11281 aagggagaca cattgaaacc aaggaaactc cacgattttc cactgtaagc cattaaagca
    11341 atccccagca gaggaagtgc aaggaaggtt atataaagca tgatgtgcat tagcgaagcc
    11401 gctttcattt gccaggcagg tggcggtggt acaatagaag gatcactata acggtgtttt
    11461 ataatcaggc gtgaaaacat taacacccaa acgaatatac ccgcattgta atgtgtttct
    11521 cgcatcagca gataagtact actaccttta ggaaaccagc cacggagttc catcgcggca
    11581 taggttattg caacgaaaag cagcgttatc caatgtaaat aaatttgagt ttttgagaat
    11641 cggttcattt ttgtagctcc tgtaattttt acagtcaagc tatcccacga accttaaaaa
    11701 atccttatac agatgtggtt ttaagaattt gttatttaaa aaattgatag tgcacattgt
    11761 aaccagccaa tataagcaat ggcgtgagca caggtatctt tctctgttgg ccgtattgtg
    11821 tatggaatcc gttattggtt aactcaatgc gggttagctt attaatatac agacacgtca
    11881 tagtgatgag cgtgtctgta gctagcttgc gttcagtggc gtttgtggat ggaccagtgg
    11941 cagttatgat gacttactaa agcggcatcg aggcgttatc atgagaatac tcaatgagac
    12001 atcatttcct cgaccacttc ttcgctactc aactgatatg gataatacgt tggccagttt
    12061 tccatttcat ttaataaaga ttgctgtgaa tcattaccca taaaaatgtg aaaatgagat
    12121 gattttcgtg gtgcaattat atgatcgcta aattgtatat atttaggggc tttgctttca
    12181 ggatctttac attcgaataa gtaacgaacg cctttcttgc ctgatttata ggtgagt
//
LOCUS       DAZGVQ010000149        12166 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000149 DAZGVQ010000000
VERSION     DAZGVQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.150"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            364..1134
                     /gene="yjjP"
                     /locus_tag="ACHLAG_004083"
     CDS             364..1134
                     /gene="yjjP"
                     /locus_tag="ACHLAG_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFP0657215.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1125..1598
                     /gene="yjjB"
                     /locus_tag="ACHLAG_004084"
     CDS             1125..1598
                     /gene="yjjB"
                     /locus_tag="ACHLAG_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFP0657216.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1705..2244
                     /gene="dnaT"
                     /locus_tag="ACHLAG_004085"
     CDS             1705..2244
                     /gene="dnaT"
                     /locus_tag="ACHLAG_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFP0657217.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2247..2984
                     /gene="dnaC"
                     /locus_tag="ACHLAG_004086"
     CDS             2247..2984
                     /gene="dnaC"
                     /locus_tag="ACHLAG_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFP0657218.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3033..3527
                     /gene="yjjA"
                     /locus_tag="ACHLAG_004087"
     CDS             3033..3527
                     /gene="yjjA"
                     /locus_tag="ACHLAG_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFP0657219.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3781..6072
                     /gene="opgB"
                     /locus_tag="ACHLAG_004088"
     CDS             3781..6072
                     /gene="opgB"
                     /locus_tag="ACHLAG_004088"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFP0657220.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTMWKGKTAFKDTVIDMARYKSNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6211..7233)
                     /gene="lgoD"
                     /locus_tag="ACHLAG_004089"
     CDS             complement(6211..7233)
                     /gene="lgoD"
                     /locus_tag="ACHLAG_004089"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFP0657221.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7372..8286
                     /gene="lgoR"
                     /locus_tag="ACHLAG_004090"
     CDS             7372..8286
                     /gene="lgoR"
                     /locus_tag="ACHLAG_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFP0657222.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8501..9862
                     /gene="lgoT"
                     /locus_tag="ACHLAG_004091"
     CDS             8501..9862
                     /gene="lgoT"
                     /locus_tag="ACHLAG_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0657223.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9911..11575)
                     /gene="tsr"
                     /locus_tag="ACHLAG_004092"
     CDS             complement(9911..11575)
                     /gene="tsr"
                     /locus_tag="ACHLAG_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFP0657224.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWAYYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TKAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11694..12140)
                     /gene="hpaR"
                     /locus_tag="ACHLAG_004093"
     CDS             complement(11694..12140)
                     /gene="hpaR"
                     /locus_tag="ACHLAG_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFP0657225.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         2969 a         3203 c         2950 g         3044 t
ORIGIN      
        1 aatcacattc atttaattta taattaaatg atatcccttc ccgcagaatg aaaaatcaca
       61 caaccaatat ttattcaatg aaatctgatg gttttaactt gttaacatta aaaaacgtgc
      121 ccggttatct attatcctga ttcgtaaagg ccaaacaata aattcattat agtgataata
      181 ttcatgtgat agtttgtttg cggcgagaga taattcgctt tttatcaccg agcttgtccc
      241 gataaccagg attaatcagc gcaattttga tcttaaccta ccgcaaaaca accgtgcttc
      301 atgatacaaa tggattctca ttatctgaac aatacgcagc acgtctatga caaagggcga
      361 gttatgcaaa ctgagcaaca gcgagccgta acacggcttt gtatccagtg tggattattt
      421 cttttgcaac atggtgcgga aagcgcgttg gttgatgagc tttcctcacg actgggtcgg
      481 gcgttgggga tggacagcgt cgaaagttct atctcttcga acgccatagt gctgacaacc
      541 attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc acgatcgcgg cattaatatg
      601 catgtggtga ctgaagtcca gcacattgtg attctggcgg agcaccatct gctggattac
      661 aaaggcgtag agaaacgatt tagccaaatt cagccattac gttacccaag atggctggta
      721 gccttaatgg ttggcctttc ttgtgcctgt ttctgtaaac tcaataaagg tggctgggat
      781 ggtgccgtca tcaccttctt tgccagtacg gccgcgatgt atatccgcca gctgctggca
      841 caacgtcatc ttcatccaca aatcaacttt tgccttaccg ctttcgccgc caccaccatt
      901 tccggattgc ttttgcaact tcccactttc agcaatacac ccactatcgc gatggccgcc
      961 agcgttctgc tgctggtgcc gggatttccg ttgattaatg ccgtcgccga tatgtttaaa
     1021 ggccacatca ataccggact ggcacgctgg gcgatcgcca gtctgctgac actggctacc
     1081 tgcgtcggcg tagtgatggc actgacgatt tgggggctac gcggatgggt gtgatcgaat
     1141 ttctgttagc gttggcgcag gatatgatcc tcgccgccat tcctgcggtc ggctttgcga
     1201 tggtgttcaa cgttcccgtt cgggcgttac gctggtgtgc gctgcttggc gcgattggtc
     1261 atggttcacg aatgatctta atgaccagcg ggctgaatat tgagtggtca acttttatgg
     1321 cttctatgct ggtcggcacc attggtattc aatggtcgcg ctggtatctg gcgcatccga
     1381 aagtgtttac cgtggcggcc gttatcccta tgttcccggg catatcggct tataccgcaa
     1441 tgatttcggc ggtaaaaatc agccagttag gttacagcga accgttgatg attaccctgt
     1501 taaccaactt tcttacagct tcatcgattg ttggtgcgtt atccatcggt ctttccattc
     1561 ctggattatg gttgtaccgc aagcgccctc gcgtataaaa ttgcctcgct taagcacacg
     1621 gatgagagac agcctcctct cctccgtgtg ttactataaa agttatctcc cttctcgttc
     1681 atcgttccat atttgagaaa cagtatgtct tccagagttt tgaccccgga cgtcgttggt
     1741 attgacgccc tggtacacga tcaccaaacc gttctggcaa aagctgaagg cggtgtggtt
     1801 gccgtatttg ctaacaatgc cccggcgttt tatgccgtca cgcctgcacg cctggctgaa
     1861 ctgctggcgc tggaagaaaa gctggcgcgt ccgggaagcg atgtcgctct ggacgatcaa
     1921 ctctatcagg aaccgcaagc cgctcccgtt gctgtcccca tggggaaatt cgccatgtat
     1981 ccggactggc aacccgatgc cgactttatc cgcctagcgg cgctatgggg cgtggcgcta
     2041 agagagccgg tgaccaccga agaactggcc tcattcattg cctactggca ggcggaaggt
     2101 aaagtctttc accatgtgca gtggcaacaa aaactggcgc gcagcctgca aatcggtcgt
     2161 gccagcaacg gcggactgcc gaaacgagat gtgaatacgg tcagcgaacc tgacagccaa
     2221 attccaccag gattcagagg gtaacgatga aaaacgttgg cgacctgatg caacgcctgc
     2281 aaaaaatgat gcctgcccat atcaaacctg cattcaaaac gggtgaagag ctactggcct
     2341 ggcagaaaga acaaggagcg atccgctccg ccgctctcga acgtgaaaat cgggcgatga
     2401 aaatgcagcg cacctttaac cgctccggta ttcggccact gcatcagaac tgctcctttg
     2461 agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt aagcaaagcg cgccagtacg
     2521 tcgaagagtt tgacggcaac atcgccagct tcatcttttc tggtaagcca ggaaccggca
     2581 aaaaccatct ggcggcggca atctgcaacg agctgctgct acgcggtaaa tccgtattga
     2641 tcatcaccgt ggccgatatt atgtcggcga tgaaagatac cttcaggaat agcggtacca
     2701 gtgaagaaca actgcttaac gatctgagca acgttgatct gctggtgatc gatgagatcg
     2761 gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa ccagatcgtc gatcgccgct
     2821 cttcttccaa acgcccaacc gggatgctga ccaacagtaa tatggaagag atgaccaagt
     2881 tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa cagtttgtgg gtgatcttca
     2941 actgggatag ctaccgtagc cgtgtaacag gtaaagagta ttaatccccg tttctggcgg
     3001 tatactgcga cacaattcca ccttgagtaa tgatgatgaa aactgttaaa catcttctgt
     3061 gctgtgccat tgccgccagc gcattaattt ccaccggggt gcatgctgcg tcctggaaag
     3121 atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa cagcacgaca caggaaggcg
     3181 gttggtcgct cgcgtcatta actaacttgc ttagcagcgg gaaccaggcc ttaagcgcag
     3241 ataacatgaa caacgccgca ggcattctgc aatactgcgc gaagcaaaag ctggcgtcgg
     3301 taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa gctgggcctg aacagtgaag
     3361 agcaaaaaga agacaccaac tatctggatg gcattcaggg tttgctgaaa accaaagatg
     3421 gtcagcaact caatctggat aacatcggaa cgactccgct ggcagaaaag gtgaaaacca
     3481 aagcctgcga tctggtgtta aagcaggggc tgaacttcat ttcctgaacc gcgaaacgcc
     3541 gcgttttagc tgttacccgg ctgacgcggc tctctcctgc ccttttccct gctcctggac
     3601 ggttttcccc ctgttacacg ggaagcgacc gcgatcaaaa aagtctgttt ataaactaat
     3661 tcctaacaac atcgcatttt tttgacacta gaattgcagc aatatggcga cgccattaca
     3721 ttgtgcttct caaaaagctt aaacgttggc taaacggccc ggtttagtga ggatcgcccg
     3781 ttgtcagaac tactctcttt cgcccttttt ctcgcctctg tgctgattta cgcatggaaa
     3841 gcgggacgta acacctggtg gtttgcagcc acattaacgg tgctggggct atttgtcgtt
     3901 ttaaatatca ccctgtttgc cagcgactat tttactggcg atggtattaa cgacgcggtt
     3961 ctctatacct taaccaacag cctgaccggt gctggcgtca gcaaatacat cctgccgggt
     4021 atcggcattg tgctggggct gacagcggtg ttcggtgcgc tgggctggat cctgcgccgt
     4081 cgtcgccatc atccgcacca ttttggttac agcctgctgg cgctcttact ggcgctgggt
     4141 tcggtggacg ccagcccagc atttcgtcag ataacggaac tggtgaaatc ccagtcacgc
     4201 gatggcgacc cggactttgc ggcttattat aaagagccgt cgaaaactat ccctgacccg
     4261 aaactcaacc tggtttatat ctacggcgaa agtctcgagc ggacctattt tgataacgaa
     4321 gctttcccgg atctcacgcc tgaactgggc gcgttgaaaa atgaaggcct ggatttcagc
     4381 cacacgcagc agctgccagg aacggattac acgattgcgg gcatggtggc ttctcagtgc
     4441 ggcataccgc tgtttgcccc ctttgaaggc aacgcctccg cctctgtctc cagcttcttc
     4501 ccgcagaaca tctgtctggg cgatatcctg aaaaactcgg gttatcagaa ctatttcgtg
     4561 cagggcgcga atctgcgttt tgccggtaaa gatgtgttcc tgaagtcgca tggcttcgac
     4621 cacttatacg gctcagaaga gctgaaaagc gtggtggccg acccgcacta tcgcaacgac
     4681 tggggattct acgacgatac cgttctcgat gaagcgtgga aaaagtttga agagctttcc
     4741 cgctcaggtc agcgattctc actgtttacc ctgacagtcg atacccatca cccggatggt
     4801 tttatctcgc gtacctgtaa ccgcaaaaaa tatgattttg acggtaagcc gaaccagtca
     4861 ttcagcgcgg taagttgcag ccaggagaat atcgcggcgt ttatcaacaa aatcaaagcg
     4921 tcaccgtggt ttaaagatac cgtcatcgtc gtctcttctg accatttagc gatgaacaac
     4981 acggcgtgga aataccttaa taagcaggat cgcaataacc tgttttttgt cattcgtggc
     5041 gacaagccgc agcaagagac gctggcagtg aagcgtaaca cgatggataa cggcgcgacg
     5101 gtgctggaca ttctcggtgg cgataactat ctcggacttg gtcgtagcag tttatccggg
     5161 cagtcaatgt cggaaatctt cctcaatatc aaagagaaaa cattggcgtg gaagccggat
     5221 atcatccgcc tgtggaaatt ccctaaagag atgaaagagt tcaccatcga ccagcagaaa
     5281 aacatgattg ccttctcggg tagccatttc cgtttgccgc tgctgttgcg ggtttcagac
     5341 aagcgcgtag aaccgctgcc ggaaagtgaa tactcagcac cgctgcgttt ccagctggcc
     5401 gatttcgctc cacgcgacaa tttcgtctgg gttgaccgtt gctacaagat ggcacaactc
     5461 tgggctccgg aactggcact ctccaccgac tggtgtgtct cgcaggggca gcttggcggt
     5521 cagcaaattg tccagcatgt tgataaaaca atgtggaagg gcaaaacggc atttaaagat
     5581 acggtcatcg acatggcgcg ttacaaaagc aatgtcgata cgctgaaaat tgttgataac
     5641 gatattcgct acaaagccga cagtttcatc ttcaacgtcg ccggtgcgcc agaagaggtg
     5701 aaacagttta gcgggatttc ccgtccggag tcgtggggcc gctggtccaa cgctcagctg
     5761 ggcgatgaag taaaaatcga gtacaagcat ccgctgccga agaaatttga cctggtgatt
     5821 accgccaaag catacggcaa taacgccagc cgtcctattc cggtacgcgt aggcaatgaa
     5881 gaacaaactc ttgtgctggg caatgaagta accaccacca cactgcattt cgataaccca
     5941 accgatgccg acacactggt aattgtgccg ccggaacctg tctcaaccaa cgaagggaat
     6001 atcctcggac actcgccgcg taagctcggg atcggcatgg tggaaattaa agttgtggaa
     6061 cgtgaagggt aatttctgac tgatgtgtag gtcgcataag gtgcgaccta cacaccttct
     6121 ccggcctaca aaacatcatt gagaaacaga aaaaaacagc gcccaacact tccccggacg
     6181 ctgcatggca actttaataa tctcgcctta tcagaaagta attacaccct taatcaactc
     6241 acgattgtta atcacatcgc gctcgtaggt ttctgccagc gtggcgaacg gatagcgatg
     6301 ggttaacatc atgtcggcag tgattttccc ttctgccatc agtcgaccga ctttagcgaa
     6361 atcttccggc gtggcgttgc ggctgcccat catcgtcgtt tcttttttat ggaattccgg
     6421 atcggagaac tgcaactcac ctttaaacag gccgacaaat accaccgtgc cgccgtgacg
     6481 aatcaaattc acggtgttat tcatcgcatg ttgattacct gtcgcgtcga tcactttctg
     6541 cgccagcgaa ccaccaaact gcgcccgtag ctgcgcgtca aaatcttcgg ctgacgggtc
     6601 cagtaaaggt aattccagac gcgttgccac atgttcacgg cgcgccggac tggtatccgc
     6661 caccaccacc tgtgcgccat cggctttagc gattgccgcc gcgcccagac cgattggccc
     6721 cgccccgacc accagcacct gctcgccggg agcaatggct gcgcgacgca ccgcatgagc
     6781 gctaatagcg aaaggttcaa tcaatgctgc cgcctgcggg tcaataccgt ctgcgggcaa
     6841 aatgttcgcc accggcacgc tcaaatactc actaaaaccg ccatcctgat gcacgccaat
     6901 gactgaaatt ttttcacagc aattggtacg cccgctttta cacgccgggc attgctgaca
     6961 ggcaacataa gggatcacag caacttgctg accattttta agatcagcaa tatttttacc
     7021 cagcccaaca atctccccac atatttcatg gcctaaaaca cgtggataac taaaaaatgg
     7081 ttgatttcca ccccaggcat gaatatcggt accgcaaatc ccgacagact taatttttat
     7141 taatgcttca ttgtcacccg gaataggtat ctcgcgttgt ttccagacta attctttcgg
     7201 ctgctggcaa attaaaacat tcatcgtaga catgattcgg ctctccattt tttgttgcct
     7261 tagatatcaa tgaaaatgaa aaatggcagc ggaacggaaa ctctgttttg tgaaaacaca
     7321 cgcataaaaa tgttttaaat cgggttttaa tggactcaca aggaggaaaa tatgagtcgt
     7381 tcacaaaatt tacgccacaa tgtgattaac caggtcattg atgatatggc gcggggtcat
     7441 atcccttccc cacttccatc gcaaagtgcg ctggcggaaa tgtacaacat cagccgcacc
     7501 acagtgcgtc atattctcag ccatttacgc gaatgcggcg tcttgacgca ggttggaaac
     7561 gactatgtta ttgcgcgtaa acctgaccac gatgacggtt tcgcctgtac taccgcttca
     7621 atgagtgaac aaaacaaagt gtttgagcag gcttttttca cgatgataaa ccagcgccag
     7681 ttacgcccag gggaaacttt ttctgaactg caactggctc gggccgcggg tgtcagcccg
     7741 gtggttgtaa gagaatatct tttaaaattc ggccgttata atcttattca aagcgaaaag
     7801 cgcggccagt ggagcatgaa gcaattcgat caatcctacg ccgagcagct gtttgagcta
     7861 cgcgaaatgc tcgaaacaca ttcacttcag cactttctca acctgcctga tcatgatccc
     7921 cgctggctgc aggcgaaaac catgctcgaa cgtcatcggc tactgcgtga caacatcggc
     7981 aatagctttc gcatgttctc gcaacttgac agagattttc actccttact gctttcagct
     8041 gccgacaata tcttttttga tcaatcgctt gagatcatct cagtcatctt tcattttcac
     8101 tatcaatggg acgaaagcga tcttaaacag cgcaatatca ttgccgtcga tgagcatatg
     8161 accattctca gcgctctgat ttgccgcagt gaccttgatg ccacccttgc gctccgtaac
     8221 catttgaatt cagctaaaca atcaatgatt cgatccatta atgagaacac gcgttatgct
     8281 cattaagtac ggattaaaaa cagcaaacta ttagcgggtg cacggtttta tcaaaagata
     8341 aataccggga aattcccgct tacctatgct cacaatcagt cgactaaaaa tgtttttgtg
     8401 agcggtagta aagtcctaaa actttaacct gccgttaatg tcaaccttgc gtaattcaca
     8461 attacgtaaa caattaaaat tataaataca ggagtccggc gtggaaaaag aaaatatcac
     8521 cctcgatccg cgttcttcat ttactccatc ttcgtcggca gatattcccg tgccaccaga
     8581 tggattagtt caacgcagta cccgaattaa acgcattcaa accaccgcca tgttgttatt
     8641 attttttgcg gcggtaatca attatctcga ccgcagttcg ctgtcggtag caaatttaac
     8701 gattcgtgaa gaattgggat taagtgccac cgaaatcggc gctttgctct ccgtgttttc
     8761 actcgcttac gggattgcgc aacttccttg cggcccacta ttggatcgta aaggcccacg
     8821 cctgatgctg ggactgggga tgttcttctg gtcactgttc caggcaatgt ctggcatggt
     8881 gcacaacttt acgcagttcg tgttggtgcg tatcggtatg gggattggtg aagcgccgat
     8941 gaacccatgc ggtgtaaaag tcattaacga ctggttcaac atcaaagagc gcggacgccc
     9001 gatgggcttc ttcaacgcag cttctaccat tggcgttgcc gtaagcccac cgattctggc
     9061 ggcgatgatg ctggtgatgg gctggcgcgg gatgtttatt accattggtg tactggggat
     9121 ttttctcgcc atcggctggt atatgctcta tcgcaaccgc gagcacgtag aactgactgc
     9181 cgttgaacaa gcttatctca atgcaggtag cgtcaatgcc cgccgagatc cgctcagttt
     9241 tgccgaatgg cgcagcctgt tccgtaaccg tacaatgtgg ggaatgatgc tcggattcag
     9301 tggcatcaac tacactgcgt ggctgtatct ggcctggctt cctggttacc tgcaaacagc
     9361 ctataacctg gatttaaaaa gcacagggtt gatggcggct atccctttcc tgtttggggc
     9421 tgccgggatg ctggtcaacg gttacgttac tgactggctg gtcaaagggg gaatggctcc
     9481 gattaaaagc cgtaagatct gcattattgc cgggatgttc tgttctgccg cctttacgct
     9541 gatagtacca caagcgacaa catccatgac ggcggttctg ctgattggca tggcactgtt
     9601 ctgtattcac tttgccggaa catcctgctg gggcttgatc cacgtcgcag ttgcttctcg
     9661 catgactgcg tcggtgggca gtatccagaa ctttgccagc ttcatctgcg cctcttttgc
     9721 gccgatcatt actggtttta ttgttgatac cacccactca ttccgtctgg cactaatcat
     9781 ctgcggttgc gtcaccgcag cgggggcact ggcgtacatc ttcctggttc gtcagccgat
     9841 caacgaccca cgtaaagatt aatctcccta tgcccgataa cattttgctt atcgggcatt
     9901 tttatggcga ttaaaacgtt tcccagttct ccccgctatc tgccactgcc attttacgcg
     9961 gcgtagctgg cgtcacggtt tttaccacag ccgatgtttc acgctgctgt tgctgctgtt
    10021 gctgaatccg gaacactgcc acagctttgg tcaggcgact ggcctgctct tccagcgcgg
    10081 cggcggcggc ggcagattct tccaccagcg cggcgttctg ttgagttacc cggtccatct
    10141 cagcaaccgc taagccaacc tgatcgatac cacggctctg ctcatcagaa gcagaagcta
    10201 tttcgcccat aatgtccgtc acgcgggtca cagcactgac aatctccgcc attgtttccc
    10261 cggcgctttc gaccagcgta gagccaacat ccactttccc caccgagtct tcaatcaggc
    10321 ttttaatttc acgagccgcc tgggcgctac gctgggccag attacgcact tctcccgcaa
    10381 ccaccgcaaa accgcgccct tgctcacccg cacgcgcagc ctcaaccgcc gcgttcaaag
    10441 ccagaatatt ggtctggaag gcaatgccgt caattacgct gataatatcg gcgattttct
    10501 gcgaactggt ggagatatcg cgcatagtct gcaccacgtt atccaccact ttgccgccgc
    10561 gttgcgccgt ttcagaagca cttaacgcca gatggctggc ctggcgcgca ttttcggcgt
    10621 tctgcttcac cgttgcggtc agttgctcca tgctggctgc cgtctcttcc agcgaagcgg
    10681 cctgttgctc ggtgcgcgaa gagagatcgt tattgccggt ggcgatttcg ctggcaccgc
    10741 tatagatggc attggccccg ttgcgcacat caccgacggt acgcatcagc tctccctgca
    10801 tatggcgcaa actctctgcc agttgcccca tctcattaga gccatccacc tcaatcggtt
    10861 tcaccagatc gccgcctgca atatgacgaa tgctgtcaat caggcgattc attggcgcta
    10921 ccagcgaggc tttaataccg aaccagacgg cgaagatgac cgccagtacg acgatcatca
    10981 cgcccaccag aatccacata gcctggctgt aggaggcatt gttatcgctg acggcgatat
    11041 catagagccg atcgttttgc tccatgtaag ccacatactg cttctcgaaa ccgtcctgat
    11101 atccctgggt cggctgatca aagaactcgt tgattttgcc tgcacctaat agttggatca
    11161 gctccgccag cgcattgtga taaatatcgt aattacgttt gatctctgcc gctgcggcgg
    11221 tgctctgacg cgggtcacgc ggcaacgctt cgtaatacgc ccagtttttt tccgcctgtt
    11281 tcagcgaaat actggcactc tgcatcagct cagcaacggt tgaaccgcta ccaatattat
    11341 tctgatccat catgtagcgg atacccgcgc ggttgagggt gttacgcgtc tgcaacaacg
    11401 cgacccagct gccattcagc gtggattgct gctggcgaat ggtttgtaaa acagtgaaat
    11461 tttctttgtc attctttaag gcattaaaga acagaccgcc tgatgtcagt tgtaaaaggc
    11521 caaaaacagc caaaaccagc agtaagctgg tcacgatctt gatacgtttt aacatggttt
    11581 ctctttcctg tggaccgatg caagattttc ggcctggaaa gcaaaaactt tatgaaaagg
    11641 tgtaacccgc catccatgga aaaaccaggc gctggttatc agatactaaa aagttattca
    11701 ttatcgcccg ggatgtcttc ttgtcgggaa ttgcccagag caataaattc ctctaacaga
    11761 tgggttaact gttgcatttt ctcggcagta aactgagctt caatctgccg ataggcttct
    11821 tcaatctgtg tctgagcgcg gttatacagc gcctgcccct ctttggttag cgagatatag
    11881 agcttccgct gatcattgat cggctttaat cgcaacacta aaccgtcgcg ttccatccgc
    11941 gtaaggatcc cggtcaggct tgggcgcaaa atgcaggcgc gatacgccag atcgtgaaaa
    12001 tccattgatg ggctttccgc cagaatacgc acgatgcgcc actgttgctc agtcaaatta
    12061 tgccgtttaa cgattgggcg aaaataactc atcgccgctt ctcgcgcctg gagcaacgca
    12121 atggttagag agtcgtgcat tatctttccc ctgaaaaatt caaaat
//
LOCUS       DAZGVQ010000150        12087 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000150 DAZGVQ010000000
VERSION     DAZGVQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.151"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(75..848)
                     /gene="uxuR"
                     /locus_tag="ACHLAG_004094"
     CDS             complement(75..848)
                     /gene="uxuR"
                     /locus_tag="ACHLAG_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFP0657226.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1063..2523)
                     /gene="uxuB"
                     /locus_tag="ACHLAG_004095"
     CDS             complement(1063..2523)
                     /gene="uxuB"
                     /locus_tag="ACHLAG_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFP0657227.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2604..3788)
                     /gene="uxuA"
                     /locus_tag="ACHLAG_004096"
     CDS             complement(2604..3788)
                     /gene="uxuA"
                     /locus_tag="ACHLAG_004096"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP0657228.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4128..5471
                     /gene="gntP"
                     /locus_tag="ACHLAG_004097"
     CDS             4128..5471
                     /gene="gntP"
                     /locus_tag="ACHLAG_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFP0657229.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(5714..6616)
                     /gene="fimH"
                     /locus_tag="ACHLAG_004098"
     CDS             complement(5714..6616)
                     /gene="fimH"
                     /locus_tag="ACHLAG_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFP0657230.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(6636..7079)
                     /locus_tag="ACHLAG_004099"
     CDS             complement(6636..7079)
                     /locus_tag="ACHLAG_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0657231.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(7152..7682)
                     /gene="fimF"
                     /locus_tag="ACHLAG_004100"
     CDS             complement(7152..7682)
                     /gene="fimF"
                     /locus_tag="ACHLAG_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFP0657232.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFCILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(7692..9729)
                     /locus_tag="ACHLAG_004101"
                     /pseudo
     CDS             complement(7692..9729)
                     /locus_tag="ACHLAG_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(9796..10521)
                     /gene="fimC"
                     /locus_tag="ACHLAG_004102"
     CDS             complement(9796..10521)
                     /gene="fimC"
                     /locus_tag="ACHLAG_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP0657233.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKRENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(10558..11097)
                     /gene="fimI"
                     /locus_tag="ACHLAG_004103"
     CDS             complement(10558..11097)
                     /gene="fimI"
                     /locus_tag="ACHLAG_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0657234.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSEHVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(11162..11710)
                     /gene="fimA"
                     /locus_tag="ACHLAG_004104"
     CDS             complement(11162..11710)
                     /gene="fimA"
                     /locus_tag="ACHLAG_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP0657235.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATHTDVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         2881 a         3132 c         2973 g         3101 t
ORIGIN      
        1 aaagtaatac tcattgcgac ttcttaattt aagaaagcgc aaggaacgtt tacccttgcg
       61 cttattataa taagtcaggc gtcgacttta tccagcggcc atgaatcaaa cagatagcca
      121 tcaaaataaa tatcgtcaac gttcgagaat tccagcagac gttgcttaac gttttccaga
      181 tgctgccaca ttgccagctt cgccgctcgg gcatcttttt tgattaacgc ggcgaggatc
      241 tgtttgtgat cgcccaacca ctctttgcga tacaggctgt catccagatg gctgtgcaac
      301 tggatccaca ttggattgtt ttcccgccac tgccaggact gacggaacag ctccaccagc
      361 atgctgttat gcgttgcttc ggcaatagcg agatggaact gcatgtcacc gctttcgctg
      421 ctgcctggcg cactggaagc cagttcacgc tcttccagtt gcaatgcctg acgcattttg
      481 acgatatctt cgcgggtagc ctgcaaagcg gcaaactcgg cgatgttgct ctccaataac
      541 tgccgcgcct gtaacagctc aaaaggaccg gcatcgttgc agacgttggc atccggactg
      601 tctgtgttct ggctgcctga gttatcaaga acatagatac cggcaccccg gcgtacttcc
      661 accagccctt tgatctccag catgatcagc gcttcacgta ccaccgtccg cgtgacatca
      721 agcatttctg caatttcacg ctccggcggc agccgttcgc cagggttgta cggcgtcttt
      781 atgatcagat cgcggatcat cgccccgact tcctggtaag gtctttgcgc agaggtggca
      841 gatttcatgg tgttgtgtcc gttaggtgcg gttaatcaaa tttttgccgg atgcggcgtg
      901 aacgccttat ccggcctaca acgggcaatt ttacggcccc cgttaatctc aaactatacc
      961 attagcactg gaagttggca cgtacaggtg ggcaaaaaaa cgaaaactgg tcaaccaaat
     1021 atcagtttga ttgaccagta agtctgtagg gtaatcagtt agttacagcg cagccacaca
     1081 ctcgcgcgca ccgcgttcgc acagctgctg atatgccgcc gtcactgcgc caacaaagtc
     1141 ggcattctgc ggcagatcat cggcaaaaat accgctcagg cccagcagcg ctttcacgcg
     1201 gtctgcgccc tgatactgcg cgttaatctt ctggaactcg gccagcatcg ggtcgaccac
     1261 gtcaatggca ttaccctgct catccacgcc ctgggtgtaa cgcatccagc cagccacgcc
     1321 cagcgccagg tgacgccagc taccgccgtt ttgcaggtgc agacgcaccg gatccagcag
     1381 acgctgcggt aacttctggc tgccgtccat tgcaatctgc caggtacggt gacgcagaga
     1441 tgggttgctg aaacgctcga tcagcagcgt cgcataggcg ttcaggtccg taccttccgg
     1501 catcgacagc gttggcgctt gttcctgcat catcagggca aaggccgctt tgcgataatc
     1561 cgggttagtc atggtgtcgg caatagtttc atagccgccg aggtaaccga ggtacgccag
     1621 gaaagagtgg ctgccgttca gcatacgcag cttcatcatt tcgaacggca caacgtctgc
     1681 aacgaactgt gcgcccactt tatcccagtc cgggcgacca ttaacgaagt tatcttcaat
     1741 cacccactga cggaacggtt cgcaggcaat ggcgcacggg tcgtaaacac ccagctggtc
     1801 agcaatttcc tgtaaggttt ctggcgtcgc cgccggaacg atgcggtcaa ccatggtgca
     1861 tgggaaggtg acgttctctt caatccatgc cgccagctgc ggatcgcgtg cctgagccag
     1921 tcccagtacc gcgaccttcg ccacatgacc gttttcacgc acgttatcgc aggacatcac
     1981 cgtaaacgct ttcaaccctt tttcacgacg cagacgcaag gcttcgacga tgtaaccaat
     2041 cgcggacttc ggcgcagtcg ggttttccag atcgtgcttg atcagtgggt tattgagatc
     2101 cagttgaccg cttgccgcat cagcgcagta gcctttttcc gtgaccgtta gagagacaat
     2161 cgccgtttgc ggacgcgcca tcgcgttgag aataccttcg cagccatcga tttccggatg
     2221 cagcgcttct ttcatcgaac cgataatttt cagctcggtg ctctctgcgc ctttttccgc
     2281 tacggtgtac agcagttgct gttttttcag gttttcgatc agcacgcggt cgttgcctgg
     2341 catcaggtta acttcgcaga tgccccagtc gctgtcggtg ctttccagca gatgatgggt
     2401 atacagcgcc tggtgcgcgc ggtgaaacgc cccgcaaccg agatgcacaa tgcgtgattc
     2461 cagacgagaa tgatcccatg acgggcgggc aaccggcaga ttgctgtcaa caatagtagt
     2521 catcgtaaac tccagaatcc gtcttcaaag agtgacggca aggaagaagg aaccggcacg
     2581 gcgcgcagcc atgccggtgg atattaacgg ctaaagaaag cgcgctggat cgccagttcg
     2641 acaccgcgaa cttcggccag gcctttcaga cgaccaattg cggagtaacc tgggttggtt
     2701 ttcttcttca ggtcgtccag catctgatga ccgtggtccg gacgcatcgg gatcaggtct
     2761 tctttgcctt ccgctttacg acggtgttct tcttcaacaa tcgctttcac cacttcgtac
     2821 atatcaacgt caccgttcag gtgcgccgct tcgtggaagg ttttcgggtt atcttcacgc
     2881 atggtggagc gcagatgggt gaagtaaata cgcggaccga actgcttgat catatcaacc
     2941 agatcgttgt cagcacgcac gccgtaggaa ccggtgcaca tggtaaaacc gtttgccatg
     3001 ctgtttacgg tatcaaccat ccactgcata tcttcaatgg tggaaacaat gcgcggcagg
     3061 ccgaggatcg ggcgcggcgg atcgtccggg tgaacagcca tacgcacgcc aacttcttcg
     3121 gcaaccggaa taatcgcttt caggaagacg gcaaagtttt cgcgcagttt ggctttgtcg
     3181 atatctttgt acagctccag gtgcttacgg aactggtcga gggtgtaccc ttcttccgcg
     3241 cccggcagac cagcgatgat gttacgggtc agacgcgctt tgtcttcgtc gctcatagtg
     3301 gcgaagcgtt cagctgcctg agcaatttct tcttcggtgt aatccgcttc cgcgcctgga
     3361 cgtttcagga tatgcatttc gaatgcagcg aattcgatct ggtcgaagcg cagagctttg
     3421 gagccgtctg gcagcacgta ttcgaggtca gtacgggtcc agtcgagcac cggcatgaag
     3481 ttgtagcaca cggtgcgaat gccacactgc gccaggttgc gcagagtctg ctgatagtta
     3541 gcgatccact gctcatagtt gccagtgtgg gttttgatat cttcgtgaat tggtacgctt
     3601 tctacgacag accacaccag gcctgcgtct tcaacgatcg ccttgcgttt gaggatctct
     3661 tctacggacc atacttcgcc gttcgggata tggtgcagcg cggtaaccac gccagttgcg
     3721 cccgcctgac ggacatcagc taaagaaacc ggatcgtttg ggccgtacca gcgccaggtc
     3781 tgttccatga tttttcctct ttcacatagt gggttccaca gcaaagagac gcggaaccct
     3841 ggagttgttt aggtatccgg atgaacaacc agaccgcagc cgtggcgttg catcggatga
     3901 gatggcgtat aagttctacc ttttggtaag acatgtcaac ccgaattctg aaattggtta
     3961 accacatcac aagaatttta ctctcctggt tgttgctgca aattgcagca aagggtaatc
     4021 gatggttatt gatcagagga tgtgacattc atcgcaacaa tggttgacca atttacataa
     4081 catgtcgccc aaaataacaa cggttcaacc aggtaaggta aaacgttatg catgtgctta
     4141 acattctctg ggtggtattc ggcattggtc tgatgctggt actgaatttg aagttcaaaa
     4201 tcaattcaat ggtggctttg ttggtggcgg cgctgtccgt cgggatgctg gcgggcatgg
     4261 atttgatgtc gctgctgcac accatgaaag cgggcttcgg caacacgctg ggggaactgg
     4321 ctatcatcgt ggtgttcggt gcggtcatcg gtaaattgat ggtcgactcc ggcgcggctc
     4381 accagatagc gcatacgctg ctggcgcgtc tcggtctgcg ctatgtacag ctgtcggtga
     4441 ttatcatcgg cttgattttt ggtctggcga tgttctatga agtggccttt atcatgttag
     4501 cgccgctggt tattgttatt gccgccgaag ctaaaattcc gttcctgaaa ctggcgatcc
     4561 cggcagtagc agctgccact accgcacatt cactgttccc accgcagccg ggtccggtgg
     4621 cgctggtgaa tgcttatggc gcggatatgg ggatggttta tatctatggc gtactggtga
     4681 cgatcccaag tgtaatctgc gcaggtctga tcctgccgaa gttcctcggc aatcttgagc
     4741 gcccaacgcc atcattcctg aaagcagatc aaccggtaga tatgaataat ctgccctctt
     4801 tcggcgtttc gattctggtg ccgctgatcc cggcgatcat tatgatctct accaccatcg
     4861 ccaatatctg gctggtaaaa gatacccctg cctgggaagt ggttaacttt atcggttcct
     4921 cgccgattgc aatgtttatt gcgatggtgg ttgcattcgt actctttggc accgcacgtg
     4981 gtcatgacat gcagtgggtg atgaacgctt ttgaaagcgc ggtgaagagt attgcaatgg
     5041 tgattctgat catcggtgcg ggtggcgtgc tgaagcagac tatcatcgac accggcattg
     5101 gcgacaccat cggcatgttg atgtcccacg gcaatatctc gccctacatc atggcatggc
     5161 tgatcactgt gctaattcgt ctggcgacgg gtcagggtgt cgtttcggcg atgaccgccg
     5221 ccgggattat aagtgctgca atccttgatc cagcaactgg tcagctggtt ggcgtgaatc
     5281 cggcgctgct ggtactggcg acggctgcgg gttccaacac cctcacccac attaacgatg
     5341 catctttctg gttgttcaaa ggttactttg acctgtcagt aaaagacacg ttgaaaacct
     5401 ggggtctgct ggagctggtc aactccgtgg ttgggctgat tattgtgctg attattagca
     5461 tggtagcgta aaaggacacc agagcctgcc aatggcaggc tctgactgat gacaaagtct
     5521 aaaaatgtgc ccagacagtc ctctcgcccc tctggggaga gggttagggt gaggggaaaa
     5581 gaccagcaaa gtgcgctttg tttatgccag atgcgacgct aacgcgtctt atctggccta
     5641 caaagggcta acgtgcaggt ttttagcttc aggtaatatt gcgtacctgc attagcaatg
     5701 ccctgtgatt tctttattga taaacaaaag tcacgccaat aatcgattgc acattccctg
     5761 cagtcacctg ccctccggta cgtgcgtaat ttgccgttaa tcccagactt accgccgaag
     5821 tccctactgc tcctaacgat accgtgttat tcgctggaat aatcgtaccg ttgcgcgtca
     5881 actgtacgcc gacgccctgt gcaggtgaaa acgacgcggt attggtgaaa atcgagttgc
     5941 ccgcatctgc ggttgtgccg gagaggtaat accccaggtt ttggcttttc gcacaataaa
     6001 cggtaagagg aattggcact gaaccagggt agtccggcag agtaacggtg acatcacgag
     6061 cagaaacatc gcagccgcca gtaggcacca ccacatcatt attggcgtaa atattccaca
     6121 caaactggaa atcatcgctg ttatagttgt tggtctgtcg caaaattagc acggcaatta
     6181 atgagccagc tttaatcgcc accccgcccg cactgctcac aggcgtcaaa taaagcgcca
     6241 ccggccacgg cttatccgtt ctcgaattat aaacaacgcg cggcgtttcg ctggtggtag
     6301 gaaatggata gctactgcca ctatatttta cggtcccgga aaaattagat aacacgccgc
     6361 cataagccga gcctcgttgc agtgtgacat agtctgtaat ggtttccgga taatcgttat
     6421 ggcaaaagat ttgcgtcgaa agatccacga ccaggttttg ccccacattc acggcgggcg
     6481 caaggtttac ataaacattg gcgctgccac cgccaatagg gatagcggta ccattggcgg
     6541 ttttacaggc gaatgaccag gcatttaccg accagcccat cagcagtaca gcaaacaggg
     6601 taataactcg tttcattaca atcatctctt cgggttcagc tgtaggtata ggtgatgcta
     6661 atcactgcct gaatggttcc ctgagtggct ccgccattta ccgtcaatgc tctgacctgt
     6721 aacgggaagt gcgctgattg tgaggaatca tccacctgaa ctgttttggt tgcgccagta
     6781 ttcaatgtgt tgccactgtc atcctgtagc tctaactgga tgttttgcgc ggtcccctgg
     6841 tttttataat atccggtact gtcggctgcc ccgctgaagc tggcagtgac cctcgacgtt
     6901 cccaccggac aattagtcaa ctcaagcgca acatcatgcc aggccgatgc cgccccggca
     6961 gacatcagac tgaaagaata aagatcgccg agatcaaccg tggcattggt ggtggaaact
     7021 gtgcacggtt tggcgacgac cttaccgttc accgtgatgg tgacatcggc tgcctgtatc
     7081 gtcgcacttg cgagcgccaa tattgccgcc aatacatacc cacgtttgca ccatttcatg
     7141 agcatctcca gttactgata ttcaagagtg aaggtagccg tggcattgat atgccctgca
     7201 gtgacaggca cctgtgtcgc cattagccgg gcgtaaaaat tcagcgtatt tggtttaccc
     7261 ggcgtcaggg tcgtccacga aagcgcggac gatggagcat taaggggtat ttgattttgc
     7321 tgctcattca gaagctgtat tcccagtccc gaagccgctg acaccgtatt ttcaagtgca
     7381 agcagattgg cattgtggct atctgcaacg ccggtaaacc caacttttac ggcagaaacg
     7441 gcattaccac agggtgacag caaaatacaa aatggaacaa caggagtcgt cgcgccaatg
     7501 ttgttaaatt gcttcgccgc gttttccatc agatcaacag taaaattggt tgattcagcg
     7561 gccacactac agccgttatc cctgacatag ccgcggatag taatcgtgct atccgcagcc
     7621 aaagcgtgac tcaccgccag ccacaaaaaa gcgcacagaa gataaaaagg tttgtttctc
     7681 atcacgcccc cttaacgaca ttcagctgat agctgggtta ataactgctg ctgactctct
     7741 ggtggcagtt gataattggc gacacagtga gcattttcct cttctcccca tttcacctga
     7801 acttttcccg ctaaaggcat tccgctgagg taaacctgac cattatccgc aacaatgccg
     7861 ctactctggc tactctctga tgtcaccatc gccccaaacg gcagcggctt attattgtgg
     7921 gtcagcgtca tgagcagttt tatcccaacg cgcgctttaa actctgctcg cacgatcgcc
     7981 ccacgagtgg gaacaacgtt agcaaccgcg ttatctaaat cgacgttatc agccagggta
     8041 ttggtatcca gcgccactct attttcccga tattcagtgg cataagctac cactcaaata
     8101 cagtgttgat gtgcgcccga gttgctgagt aacggtgagc tgtaatttcc cgcgtttgtt
     8161 ataagcgagg ttgtaatagt cggtgaattt cggcttaacc tgaataactc cgtcctgtgt
     8221 ttcgatgttg tagccattca ttcgactgta tgttgtatca gcgaaattaa aatatccgct
     8281 ggtcgaataa cggtaaccca ctaactgaat attcgtgcct gactcattga gcgatttgtt
     8341 atagagaaaa cgcaccgatt gtccgtcatg ctgactgtca tcgggaagtg tggaattagc
     8401 ctgcgtcata tccacagaca gagcgcccag tgcccccatg tttttcccga taccgaaatt
     8461 aaaagcacga taacgatccg ccagttgcgt tccaccatat attgtccagc cagccggaag
     8521 gccgtggagt aatgtactct ggaaaaagcg gggtttttcc tgctgcgcat ttccactacg
     8581 gtattctcct gccgtaatgg aataacgagt atgcccttca cgttgcaaaa gcgggactga
     8641 cgaatagggt acggtaaaaa tctgcgtgct gccgtcagcc tctttgatcg ttacctgcaa
     8701 gtcaccacta ttacctgcgg catagatatc gttgatggta aaaggccccg gtggcaccgt
     8761 actattataa atgtcatacc cattttgttt aatagtgacc tgtgcagtac cacgagcaat
     8821 accgtggatc accggggcaa atcctctttg gctatcgggt aacatattgt catctgaggc
     8881 caattgtgcg ccgcgaaagt taataccatc gaaaatatcg ccctgagtat aaccatcacc
     8941 cagcgtcagc cgggaacgta acggtattat gtctcgctca agccaggtat tgatatgctg
     9001 ccatttattt ttgctacctg atgatctgtc gctacggtta taactccagg tggtattgtc
     9061 gcgtaaacgc cacgcaccaa tatttaaccc actctgtagg tttaaatatg cataatggct
     9121 gttaccccca atccgattct gtacactatt tccgctgaaa ttataattga gcaatccggc
     9181 attaataccg ggatcccata actcaggagg aatataacca cgcgcgcgat tactcataaa
     9241 tgcctgaggg atcgtcaggt tgagtcgctg ctgaccaaca tctaaatgcg cagtagcgtc
     9301 atggattatt gaggttaatg gcacgcaggc atcatccgcc agcagattca taccggagac
     9361 agaagccgta ttcagcccca tactggcaag ttgcgcgcgt gtcaggcagg gaacaatccc
     9421 ttgttcgctg tcgcccgtat taaatgtgac atcacgcgtt gccatataac cattattcaa
     9481 atagatatcg acacgatacg tccctggcgg taattcttgc ccattttcaa aacgcgataa
     9541 atcagccaca gcctggggat catccgctaa aaagcgcgga ttaaaataga gttcggcaga
     9601 tgacaaaggt gtctgtgcgg caaaagcaca ggcgacaaag agccggacaa aaaaaccagc
     9661 caaacgatgc ttacgaatat gcaagcattg tgtgtttcgc tggtaaagtc ttaaattcag
     9721 atatgacatt attcctccgg tttaaaatca cccggcagtc aattcttttt attcaggcga
     9781 aaaattcccc ctgcgttatt ccattacgcc cgtcattttg ggggtaagtg cgccataatc
     9841 atttattgtt cggtaagtaa tattgcttcc tgcatcagaa ggcaatttaa ccgtgctttc
     9901 gcccattgga ggcaccaatg cattttcaag aacccgggtt ccggcattca actctgttac
     9961 cgtcaggtaa tagggtgtcg ggttaatcag cgtcagggaa ttcgcgctac gacgaaatct
    10021 taatttttct gcggcctgat cgggtggcaa cgctaattta gccggacgat agtacagttt
    10081 aatgcggctg ataattgcga gctgtagcgt attctcagtc aattttgatt tatccattga
    10141 cggaatcgct ttaacattca tccagaataa actttcccga tcctgtggca attggttatt
    10201 agttgcatca agaatacgca aggtattctc tctttttccc ttcatcgcaa acagaggagg
    10261 cgtcacgata aaacgaccat cctttacacc atcggcattt tccacccatg attgaattaa
    10321 ataggtacta ttttcatcat tatttgtcac ggcaagttgc acttgttttt gccctgccgg
    10381 ataaattacg cgagtcgcac ctaaggccac tcccgcttca gcgcgtccgg caaccatcat
    10441 tgccatgaac atcaggatac ctgccagcaa gcagaatgtt atttcctgcg atttccttac
    10501 attgacgttt ttattactca ctgtcctgtt cctgttatca cattatctgc tgaacaatta
    10561 ctgataggtt aaagagaacc aggcctgtgc attggcgatg ccgccagtaa cccgacgccc
    10621 ggtagcacga tatttggcga tgaaatgtag cgaagtagag cctgagtaaa gccgtttcca
    10681 gtttgctgga ggacgattaa tcggtacgag gtttccttca tcatcaaaca acgctacgcc
    10741 aatattggtg gctatccctg gcccctctcc aacggaaagc acatctggat ttttaccatc
    10801 cgcgacaccg tgaaacgcca cacccacatg ttcactcacc accgtgctac attcccgtaa
    10861 atgaataaca aaaggcaccg gtgcgctatc ttccccaacc gcatgaaacc ggttactgct
    10921 gatttgcccc atattgaccg tcatttgttt atcaccggct tcaatccggc aagtttccgc
    10981 aataatgacg ccctgaaatt gcatatttcc gccgggcaac gtggtattcc atttatttcc
    11041 ggccagagca aacattggca gcaacgacgc taatagaaat aatctcttcc ttttcatcgt
    11101 tatttttatc gcacaagggt gggcatccct gcccgtaatg acgtccctga acctgggtag
    11161 gttattgata ctgaaccttg aaggtcgcat ccgcattagc tgcgccaggg gttgcggcac
    11221 cggttgcaaa ataacgcgcc tggaacggaa tggtgttggt tccgttattc agggttgttt
    11281 ctgaactaaa tgtcgcaccg tccagcgcca gcgcagcacc cgttctgtcc aggatctgca
    11341 caccaacgtt tgttgcgcta cccgcagctg aactctgcag agccagtaca tcagtatgcg
    11401 tagcatcaat tgccgtacct aagaaggcaa cagcggcttt tgtggcaaca gtggtatcgc
    11461 aatcattcag ctgaatgtta aaaccaacgg cagagctggt tgctccagcc tgcttcaggc
    11521 tagcggtacg aacctggccc aactgaacgg tttgatcaac agagcctgca tcaactgcgc
    11581 aagcggcgtt aacaacttcc cctttaaagt gaacggtccc accatttacc gtcgtagtat
    11641 cggccagagc cgctgcggaa ctgagggaca gagccgacag aacaacgatt gccagagttt
    11701 taattttcat gctgctttcc tttaaaaaaa ctatttctaa atcgacatgg gcagtcgttc
    11761 tgtacacttt gttttgtcaa cgagtttgac tgccaacact gcacagtttt cccccaaaag
    11821 atgaaacatt tggggccatt ttgactcata gaggaaagca tcgcgcacaa actttttcag
    11881 tttatttgcc ggcttaatat tctattgtta tctttattta tagattttta tattgcatga
    11941 ggtggttttt ggagagaaga atgaggaaga tgcgtcgagc cacagaaacg ttagctttac
    12001 atatagcgga ggtgatgtga aattaattta caagagaaat aatttacata tcaaacagtt
    12061 agatgctttt tgtcgttttt taatatt
//
LOCUS       DAZGVQ010000151        12031 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000151 DAZGVQ010000000
VERSION     DAZGVQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.152"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(36..944)
                     /gene="galU"
                     /locus_tag="ACHLAG_004105"
     CDS             complement(36..944)
                     /gene="galU"
                     /locus_tag="ACHLAG_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP0657236.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(1146..2159)
                     /gene="rssB"
                     /locus_tag="ACHLAG_004106"
     CDS             complement(1146..2159)
                     /gene="rssB"
                     /locus_tag="ACHLAG_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFP0657237.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(2251..3156)
                     /gene="rssA"
                     /locus_tag="ACHLAG_004107"
     CDS             complement(2251..3156)
                     /gene="rssA"
                     /locus_tag="ACHLAG_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFP0657238.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            3269..3727
                     /locus_tag="ACHLAG_004108"
     CDS             3269..3727
                     /locus_tag="ACHLAG_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFP0657239.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFENCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            3777..4619
                     /gene="purU"
                     /locus_tag="ACHLAG_004109"
     CDS             3777..4619
                     /gene="purU"
                     /locus_tag="ACHLAG_004109"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFP0657240.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            4779..4863
                     /locus_tag="ACHLAG_004110"
     tRNA            4779..4863
                     /locus_tag="ACHLAG_004110"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4813..4815,aa:Tyr,seq:gta)
     gene            complement(5047..5724)
                     /gene="narI"
                     /locus_tag="ACHLAG_004111"
     CDS             complement(5047..5724)
                     /gene="narI"
                     /locus_tag="ACHLAG_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP0657241.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(5724..6434)
                     /gene="narJ"
                     /locus_tag="ACHLAG_004112"
     CDS             complement(5724..6434)
                     /gene="narJ"
                     /locus_tag="ACHLAG_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP0657242.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(6431..7969)
                     /gene="narH"
                     /locus_tag="ACHLAG_004113"
     CDS             complement(6431..7969)
                     /gene="narH"
                     /locus_tag="ACHLAG_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP0657243.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(7966..11709)
                     /locus_tag="ACHLAG_004114"
     CDS             complement(7966..11709)
                     /locus_tag="ACHLAG_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFP0657244.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVESLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         2862 a         3250 c         2955 g         2964 t
ORIGIN      
        1 tcaacgccgt ttcgtggata acaccgatac ggatgttact tcttaatgcc catctcttct
       61 tcaagccagg ctttaaattc cgtgccaagg gtgttatgac gaataccgta ttcaacgaag
      121 gcctgcatgt aacctaattt attaccgcag tcatggctct tccctttcat atgataggct
      181 tccaccgttt ctttttcgat cagcatatca attgcgtcgg tgagctgaat ttcatcacca
      241 gctcccggag gggtttttgc cagcaacggc caaatatccg cgctaagtac gtaacgaccc
      301 acaatagcga gattagacgg cgcaacatcc gctttcggtt tttctaccac accaaccatc
      361 ggtacgcttt caccaggcgc taattcaacg cctttgcaat ccacaacgcc atatgcggtc
      421 acatcagcaa ccggttcaac catgatctgg ctatgacccg tttcatcaaa gcggcggatc
      481 atctctgcca ggttatcctg tgacaaatcg gattcatatt catccagaat aacatcaggc
      541 aaaataacag ctaccggttc atcacccact accgggtgag cacacaatac cgcgtgtccc
      601 aggcctttcg ccagaccctg acgaacttgc ataatagtca cgtgcggtgg acaaatagac
      661 tgcacttcat caagcagttg acgttttaca cgtttttcca gcattgcttc cagttcaaaa
      721 ctggtatcaa agtggttttc aatagagttt ttagatgagt gtgtaaccag cacaatttca
      781 gtaatgccag ccgcaataca ttcattcacg acgtattgaa ttaatggctt atcgacaagt
      841 ggcagcatct ctttcgggat ggctttcgtc gccggcaaca tcctggttcc taatcccgca
      901 acggggataa cggctttttt gactttcgta ttaatggcag ccatttaaat tctcctggac
      961 tgttcgtgtt ttgaacgtgt tcatatttct gtatcgcatc ccagtatatc agcaccccga
     1021 aggggctaag ttctgaaaag gaggcgagtt agcataatta agacaattac ttatacgaat
     1081 ggcgacgata gtaccaccag ccgacattag caggtaatgc aaatttagcc cgcgttatcg
     1141 tttgctcatt ctgcagacaa catcaagcgc agtcgaccac cggttcccca tatttggcat
     1201 tgccaggcat cgcatcgctg gctcaattga ttcaaataag cgttacctaa agtgcctaac
     1261 ggaacaccat tactgatttg cacctggtgt tcgccggtat ttaacgtcgc attcagaccc
     1321 gcagaaacca gaatgagatt tttcagttcg cgatgataat agccaactaa tagcggaaac
     1381 tgccccggca gattggcctg acgaagtaaa tggtttacct gcttcaataa cgcgcccaac
     1441 tctggcaacc gttgattttg gtgtgcaagc tgttcctgta ataatccgtt aaacaatgcg
     1501 cgtaataaca aggcagcaag tacgccatta tgtccagctc gggtgacatc aaggcaataa
     1561 aatgccagat cgttttccga aagtgcggca atatcaagca ccaggccggg tttgtccgcg
     1621 gcaaccaatt gacgataatt aacccggcaa tgggaaatca cctgctgaac cggcggttgt
     1681 agttcctgta ataatttcgc cgctgcggca gggttatcaa ccattgcatc ccagtcgcga
     1741 aaaagccttt cctcttcctc aacgcgcgaa ttaaacatgc tgggatagag acaggcaaaa
     1801 accatctcgc gcaagcgatt cagatcttta actggtttca gcaaaacatc ttcaacgccc
     1861 agacgtaacg ctttggcaat atctgccata ttttcagtgg cagatatcac cagaaccggg
     1921 gtctggtcgc ctctgttacg tatatgctcc agcagtttaa gcccgttcat tcgtggcatc
     1981 gcgatatcac atatcatcag gtctggagtg aaacctccca gcaactcaag ggcatccacc
     2041 ccatcagccg ccagtaccgt tgtcgctccc aatgaggaaa accatgaatc cagaagcgag
     2101 cgaaatacct gctcatcttc aacaatgaga atctgttttc cgaccaatgg ctgcgtcatg
     2161 ttctctcccc tgactggctt tactcaatag tggcatgcta ttgccacctg cgcctgtcag
     2221 aattaactta agtgtagata aaaattctgg tcaaatgttg gtgcgtacca acggcaaaag
     2281 ttcgtccatt ttcctttcca ctgccagctg tccggccgca atggcagcgt gcgcgcgatg
     2341 gaaatcaagc gtagatattt gcgggcaaac aggttgaatc agaatatcgg gcggatcacc
     2401 tgccatgcgg ttccttttaa ggcggttctc cagcacctgg atagaagtgg tcataatctc
     2461 tgttgccgtt ggtgctgtca ccgcccgacg tgtcgttatg ctgcctaacc tttctttcag
     2521 acgcgcatgc cacggcagag aatcaccatt ctcgctattt tcttcactga cattaaagga
     2581 gagcaaatct tgttgcatta aatgagcatc gtgctgcagg tcgaccgcta tcacaatatc
     2641 agcacccaat gcacgcgtga gcgaaatagg aattgggtta acgactgccc catcaaccag
     2701 ccagtagccg ttatgcgcga ctggtgccat gagtcctgga atactgcatg acgcgcgaat
     2761 agcaagatgg agatcgcctt cagtaaacca taattcacgc ccagtactta aattggtggc
     2821 aacagccgcg aagcgacggg aacaattttc gatctctgtt tccggcatta tctcgcggta
     2881 ttgattgaag acacgctcgc cgcgcagtaa cccaccgcgc tgccaggaga gatccatcag
     2941 gcgtaaaaca tcccaataac tgaaagaggt tacccaatct tccagcgcag ataatcgatc
     3001 gcatgcatag gccgcgccca ccagcgaacc aattgaacat cctgcaacga tatcaatttc
     3061 aatacccact ttttttagcg cattaataac gccaatatgc gaccaacctc tcgccgcgcc
     3121 agatcccagc gccagcccta tttttatctt tctcattatt cccgcaagat tcccctggaa
     3181 tgcaatcgta gccacatcgc tactgctcgg ttaacatagt gctactcagg cggttaatac
     3241 gccgttattg tttccaggga gattatttgt gtctcagctt tgtccctgtg gtagtgctgt
     3301 cgagtatagc ctatgttgcc acccttatgt gtctggtgaa aaggttgcac ctgatccaga
     3361 acatctcatg cgttcgcgtt actgcgcttt tgtgatgcaa gacgcagatt atttaataaa
     3421 gacctggcat ccatcttgtg gagcagcggc gttacgtgcc gaattgattg ccggatttgc
     3481 gcatactgaa tggttgggat tgaccgtatt tgaaaattgt tggcaggacg ccgacaatat
     3541 cggatttgtc agttttgttg ctcgatttac cgaaggtggt aaaacaggtg caataattga
     3601 acgttcgcga tttttgaaag aaaacggtca atggtactat attgacggta cacgtccgca
     3661 gtttggtcgc aacgatccct gcccttgtgg ttcaggtaaa aaatttaaaa agtgctgcgg
     3721 ccaataatgg ttgacggtac ggtttagcaa acactctcaa caaggttttt ccagcaatgc
     3781 attcactcca acgtaaagtt ctgcgtacta tttgtccgga ccaaaaaggt ctgatcgcac
     3841 gcattaccaa tatttgctac aagcacgagt taaatatcgt acagaacaat gaatttgttg
     3901 atcaccgtac cgggcgcttt tttatgcgca cggaactgga agggattttt aatgattcca
     3961 cccttctggc ggatctcgat agcgcattgc cagaaggctc cgtgcgtgag ctgaatcctg
     4021 ccggtcgtcg ccggatagtg attctggtca ctaaagaagc gcattgcctt ggcgatttgt
     4081 tgatgaaagc caattacggc ggcctggatg tcgaaatcgc ggcagttatt ggtaaccacg
     4141 atactttacg ttctctggtt gagcgttttg atattccgtt tgagctggta agccatgaag
     4201 ggttaagccg caacgagcac gatcaaaaga tggcggacgc cattgatgct tatcaacctg
     4261 actatgtggt gctggcgaag tatatgcggg tattaacgcc ggaatttgtg gcacgcttcc
     4321 cgaataaaat catcaatatt caccattcat tcctgcctgc gtttatcggc gcacgtcctt
     4381 atcaccaggc ctatgaacgt ggtgtgaaga ttattggcgc aaccgctcac tatgtgaatg
     4441 acaatctgga cgaaggccca atcatcatgc aggacgttat tcatgtcgat catacctaca
     4501 cagctgaaga tatgatgcgc gcaggtcgtg acgtcgagaa aaacgtctta agtcgtgcgc
     4561 tatacaaagt actggcacag cgcgtctttg tttacggtaa tcggacgatt attctttaat
     4621 cgccagcaaa aataactggt tacctttaat ccgttacgga tgaaaattac gcaaccagtt
     4681 catttttctc aacgtaacac tttacagcgg cgcgtcattt gatatgatgc gccccgcttc
     4741 ccgataaggg agcaggccag taaaaagcat taccccgtgg tggggttccc gagcggccaa
     4801 agggagcaga ctgtaaatct gccgtcatcg acttcgaagg ttcgaatcct tcccccacca
     4861 ccatcgcttc cttaaataat caaaacctgt tactccgagc taattttgcc cgatggcacc
     4921 acgtttaccg ggcctacaat atcctcccct gtaggtcgga taagcgaagc gcatccgaca
     4981 actcaccccg caccgtgccc ataaaaaaac cccgccgaag cagggtctat gtgaactaaa
     5041 attcgcttag tgacgagcgc gcaccagctg gtacttacgt gtcagatact ccaccggtac
     5101 gctccagatg tgtaccagac gcgagaacgg gaacagcagg aataacgtca tccccagcac
     5161 caggtgcaga cggaagataa acgccacacc atcgaggtgt tgagaagcgc caccgtggaa
     5221 ggtcaccacc gactgcgccc agccaaccag tttcatcatc tcgctaccgt ccatatgctg
     5281 agcggagaac ggaatggtca acaggcccag cgcgcactgg ataacgagca gcgacaggat
     5341 caggatatcc gctccggtag tggttgcacg cacgcgtggg ctgaacagac gacgcttcag
     5401 caacagcacg ccgccaatca gacacagcac gccgctggca ccaccagcaa acatcgccat
     5461 tttctgtttc acttcaatcg gcagccaggc ttcatacatc cagtgcggcg tcagcatacc
     5521 gaagaagtga ccaacaaaaa tccccagaat accgatatgg aacaggttcg acgccaggtt
     5581 catccctttg cgatccagca tctggctgga cgccgcgcgc caggtgtact gcccgtagtc
     5641 ataacgcagc cagctaccaa tcaggaagac cgccccggct atgtacgggt agatatcaaa
     5701 gaagaacata ttcaggaatt gcattagtgc tgtcctccgg tggtgatatt cagatattgc
     5761 ggcgcgacgg caccggcaaa gcgacgctga tgagcagtga ttgctgaatc accgcagcct
     5821 ttgtcagcaa agaatttaac ctgctcttct tcccagacag catccagcgc ctgcggcgta
     5881 tcatcgcgcg cttcgtcggc aattttttcc gccactttgt cgctgtcgat agcggtattg
     5941 gccaatttca gcagcagatc aaacatcacg gcataacggc tttcacgctg ttgcagacgc
     6001 gcgctcagca atgccagaat cggcgcgata tctttcaaac cttccacggc ttcgctttgc
     6061 ggcagctgcg acaggtactc cagatacagc ggcagatggt ccggtaattc gcggctgttt
     6121 aactgcaagc cgtgctgctc gtactgcgcc agcaggtcca ccatcgcctg gccgcggtca
     6181 cgggattcac cgtgaacatg ttcaaatagc aacagtgacg tggcgcggcc acggtcgaac
     6241 agttcgctgt actgggcctg ggcatcgagc ggatccatcg ccgttaaatc gcgcaggaaa
     6301 atgcccagcg catgggcatc ttctttcgac agatttttcg acgcagcaat cgcctcaaac
     6361 atctcttgtt gatgctgcca taaggcagca tccggatatt caaggagacg cgatacaatc
     6421 acgagttcga tcatggatgc ggctccgttt tgctggtcac atcgatggca tcgatacgac
     6481 ggctgttgaa cagattgaat ttggtatctg aaccgtggca gccatcacca aaggtaaagc
     6541 cgcagccatt tttctccggg aaggcttccc gtgccagttc acgatgacta ctcggcacca
     6601 caaagcgatc ttcgtagtta gcaatcgcca gataacggta catctcctgt gcctgggctt
     6661 cggtcagacc gacctcttcc agcgcacggg tatcaacttt accgtcaacg gtttcagcac
     6721 gtttgtaatg acgcatcgcc agcatacgtt tcagtgcgcg cagtaccggt ttggtatcac
     6781 cggcggtcag cagattcgcc agatactgta ccggaatacg caagctttcg acgtctggca
     6841 gaatgccgtt gctacccagc tcacccgcgt ctgctgcaga ctgaatcgga gacagaggcg
     6901 gcacgtacca gaccatcggc agtgtgcgat attctggatg cagcggcagc gccagtttcc
     6961 attccattgc cattttataa accggcgact gctgtgcggc ttcaataacg ctcagcggaa
     7021 taccgtcttt aatcgcctgc tcgatgactt tcggatcgtt cggatcgagg aacacctcca
     7081 gctgacgctg gtaaagatct ttctcgttct cggtgctggc tgcacgttca atcgcgtcgg
     7141 catcgtacag cagcacgcca agataacgga tacgaccgac acaggtttct gagcacacgg
     7201 tcggctgacc cgcttcaata cgcggatagc agaagatgca cttctcagac ttaccgctct
     7261 tccagttgaa gtagattttt ttgtacgggc atccggtgat gcacatacgc cagccacggc
     7321 atttatcctg gtcgatcagg acgatgccat cttcttcacg cttgtaaatc gcaccgctcg
     7381 ggcaggtcgc aacacatgcc gggttcaggc agtgttcgca caggcgcggc aaatacatca
     7441 tgaaggtgtt ttcgaactgg ctatacatcg ccttctggat gttgtcgaag ttcttgtctt
     7501 tcgccagttt gtcaaactca ccgcccaggt catcttccca gttcggccct ttttcgattt
     7561 tcgccatccg ttccccggta atcagcgaac gcggacgggc aatcggctgc gatttgctgc
     7621 cttccggcgc ggtatgcagg ttctgatagt caaaatcgaa cggctcgtaa taatcgtcga
     7681 tccccggcag atgcgggtta gcgaagattt tacccagcaa catggcacgg ttacccatgc
     7741 gcggctgcag tttgccgttg attttacgga tccagccgcc tttgtatttt tcctggtttt
     7801 cccagtcagt cgggaagccc tgacccggct tggtttccac gttgttgaac cacgcgtatt
     7861 ccacgccttc acggctggtc cagacgtttt tacaggtaac tgaacaggtg tggcagccga
     7921 tgcacttatc gagattcagc accatgccga cttgtgaacg aattttcatt ttacgctctc
     7981 ctgtacctgg tcattgcctt cgccatctaa ccagtcaatg ttcttcatct tacgcactac
     8041 aacaaactca tcgcggttag aacctacggt gccgtagtag ttaaagccgt atgccagatg
     8101 ggcatagccg ccgatcatat gcgtcggttt cggcgtgata cgggtgaccg agttatggat
     8161 accaccacgc tgttgggtaa tttccgaacc aggcaggtta acgatacgtt cctgcgcgtg
     8221 gtacatcatg gtcatccctg ccggaacacg ctggctgaca accgcacggg cagtcagagc
     8281 accgttgctg ttgaagactt caatccagtc gttatcggcg atacccagat ctttggcatc
     8341 ggcttcactc aaccagacca ccggaccacc gcgacctaaa gtcagcatca gcaggttgtc
     8401 gctgtaggtg gagtggatac cccacttctg gtgcggcgtc aggaagttga gcgctttttc
     8461 cgggttgccg ttggatttct ggcccatcac ttctttcacc gaacgggtgt cgatcggcgg
     8521 acgataaacc agcaggcttt caccgaaatc acgcatccat tggtgatcct gatacagctg
     8581 ctgacggcca gagagcgtac gccacgggat cagctcgtga acgttggtgt aaccggcgtt
     8641 gtaagaaacg tgttcatctt caaggccaga ccaggtcggg ctggagatga ttttgcgcgg
     8701 ctgtgcctga atatcgcgga agcgaatctt ctcgtcttct ttattcagcg ccagatgcgt
     8761 atggtcacga ccggtaaact cgctcagggc agcccaggct ttcacggcta cctgaccgtt
     8821 ggtttccggt gccagcgtca ggatcatctc tgccgcatca attgcggtgt tcagcatcgg
     8881 ctggcctttc gccggacctt ccgctttggt gtagttgagc ttacgcagca gatccatctc
     8941 gctctgggtg ttccaggcaa tccctttacc gccattaccg attttctcca tcagcgggcc
     9001 gatagaggta aagcgttcgt aagtcgccgg ataatcgcgc tctacgacca taatgtgtgg
     9061 cgcggtttta ccagggatca ggtcgcactc gccttttttc cagtctttca catccagcgg
     9121 ctgcgccagt tcagcggcag agtcgtgctg gataggcagc gtgacgatgt cggtttcttt
     9181 acccagatga ccgacgcaca cttcggagaa tttcttcgcg atggctttgt aaatttccca
     9241 gtcgcttttc gcttcccagg ccggatcgac cgccgcagac agcgggtgaa taaacggatg
     9301 catatccgaa gtattcatgt cgtctttttc gtaccaggtc gccgtcggca aaatgatgtc
     9361 ggaatagaga caggtgctcg acagacggaa gtccagcgta accaccagat ccagcttgcc
     9421 ttccagacca ttgtcctgcc agtccacttc ttccggcttc acgccgccct gttgccccag
     9481 atctttcccc tggataccgt gctccgtccc cagcaggtac ttgagcataa actcatgacc
     9541 tttaccggaa gaaccgagca ggttagaacg ccagatgaac aggttgcgcg ggtggttttt
     9601 accgttttct ggttgttctg ccgcaaaacg gatggaaccc tctttcaggg attccaccgt
     9661 atagtccacc ggattcatcc cggctttttc cgcttcgcca gcgatagtca gcgggttagt
     9721 gcctaactgc ggtgcagacg gcagccagcc catgcgttcc gcacggacgt taaagtcgat
     9781 caagtgtccg gtatagcggg atttgtccgc catcggtgac agcaactctt ccgccgtgac
     9841 ggtttcataa cgccactggc tggagtggtt atagaaataa gaagtgctgt tcatgtgacg
     9901 cgccggacgc tgccagtcaa gggcaaacgc cagcggctgc cagccggttt gcggacgcag
     9961 tttttcctga cctacatagt gcgcccagcc gcccccgctc tgaccgacac agccgcagaa
    10021 aatcagcatg ttgatcagac cacgatagtt catatcgagg tgataccagt ggttcagccc
    10081 cgcaccgacg ataatcatcg aacgaccgtg cgttttatca gcgttatcgg caaattcacg
    10141 ggcgatgcga ataatctggc tgcgagaaac gccggtaatc tgctcggccc aggccggggt
    10201 ataagctttc acatcgtcat agctggttgc acagttaacg tcgttcaggc cacgttccag
    10261 accgtagttt gccagcgtca gatcataaac ggtggtcacc agggcggtgc tgccatcagc
    10321 cagttgcagg cgtttcaccg gcagtttgtg cagcagcacg ttttccagtt ccactttgtt
    10381 gaagtgttcc gtgccgtcgc caccaaagta cgggaaacct acctcagcga tctcatcctg
    10441 gctacccagc aggctcagtt gcagctcggt ttcttcgcca gttttgccgt cgcgctgctc
    10501 aagattccat ttgcccttct cgccccagcg gaagccaata gaaccgttcg gcgcaaccat
    10561 ttcgccattg gtattaaagg cgacagtttt ccattccgga ttgttttcct ggcccagcgc
    10621 atcaaccaga tcagcagcgc gcagcatacg acctgcagcg tagtaaccgt cgcgttcttc
    10681 cagcatcacc agcatcggca tgtcggtgta gcgacgcaca tagtcggtga aatactggct
    10741 tgggttgtcg aggtggaatt cacgcagcat tacgtggccc atcgccagcg ccattgccgc
    10801 atcggtgccc tgtttcggtg ccagccacag atcgcacagt ttggcgattt cagcgtagtc
    10861 tggtgtgacg gcaacagttt tggtcccttt gtaacgcact tcagtaaaga agtgagcatc
    10921 cggggtacgc gtctgcggca cgtttgaccc ccaggcgatg atgtagctgg agttgtacca
    10981 gtcagcagat tccggtacgt cagtttgctc gccccaggtt tgcggagacg caggaggcaa
    11041 gtcgcagtac cagtcgtaga agcttaagca agtaccgcca atcagcgaga gatagcgtgc
    11101 acccgatgcg taagaaacca tcgacattgc cggaattggc gagaagccag cgacacggtc
    11161 cgggccgtag tttttgatgg tgtaaacgtt agatgcggcg atcagttcgt tcacttcctg
    11221 ccaggaagaa cgaacaaaac caccgcgtcc acgcgcctgc ttaaagcttt tcgctttatc
    11281 ggcgtcttca atgatagaag cccatgcctc aaccggatcg ctatgcagcg ccttcgcttc
    11341 acgccacatt ttcatcaggc gtttgcgcat catcgggtat ttcaggcggt tggcgctgta
    11401 aagataccag gagtagctgg caccgcgcgg gcagccgcga ggttcatggt ttggcagatc
    11461 cggacgggta cgcggatagt cagtctgctg ggtttcccag gtgaccagac cgtttttgac
    11521 gtagattttc cagctgcagg agccggtgca gtttaccccg tgggtagagc ggacgatttt
    11581 gtcatgctgc caacgctggc gatatccatc ctcccagtca cggttggtat tgagaagctg
    11641 gccatgccca tcggcaaagg tttcaccctt ctgtttgaag tagcgaaacc ggtccaggaa
    11701 tttactcatc ggttttctcc tgtgggagcc tgtcggccct ctgataaatc gacattgcta
    11761 atgtttaagg gcaaggtaac gctctgaaac agcgtgggaa ttgataacga tcaagtgtga
    11821 tggggatgaa aaataaagta aattcctaag ataccccttt aggataatta acagttaaag
    11881 cataacatgc tgtaaattaa gacgttttat acctgtagtt atttaaagca tgaagatttt
    11941 tatgcagatg ggtattaagg agtattcccc atgggtaaca tattaatggc tcttaatgaa
    12001 atccggcgaa ataagccata taacattagt a
//
LOCUS       DAZGVQ010000152        11924 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000152 DAZGVQ010000000
VERSION     DAZGVQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.153"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            118..4080
                     /gene="putA"
                     /locus_tag="ACHLAG_004115"
     CDS             118..4080
                     /gene="putA"
                     /locus_tag="ACHLAG_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP0657245.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(4120..4758)
                     /gene="rutR"
                     /locus_tag="ACHLAG_004116"
     CDS             complement(4120..4758)
                     /gene="rutR"
                     /locus_tag="ACHLAG_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFP0657246.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            5046..6137
                     /gene="rutA"
                     /locus_tag="ACHLAG_004117"
     CDS             5046..6137
                     /gene="rutA"
                     /locus_tag="ACHLAG_004117"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFP0657247.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            6137..6829
                     /gene="rutB"
                     /locus_tag="ACHLAG_004118"
     CDS             6137..6829
                     /gene="rutB"
                     /locus_tag="ACHLAG_004118"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFP0657248.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            6841..7227
                     /gene="rutC"
                     /locus_tag="ACHLAG_004119"
     CDS             6841..7227
                     /gene="rutC"
                     /locus_tag="ACHLAG_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFP0657249.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLEIIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            7235..8035
                     /gene="rutD"
                     /locus_tag="ACHLAG_004120"
     CDS             7235..8035
                     /gene="rutD"
                     /locus_tag="ACHLAG_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFP0657250.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            8045..8635
                     /gene="rutE"
                     /locus_tag="ACHLAG_004121"
     CDS             8045..8635
                     /gene="rutE"
                     /locus_tag="ACHLAG_004121"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFP0657251.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            8646..9140
                     /gene="rutF"
                     /locus_tag="ACHLAG_004122"
     CDS             8646..9140
                     /gene="rutF"
                     /locus_tag="ACHLAG_004122"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFP0657252.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            9161..10489
                     /gene="rutG"
                     /locus_tag="ACHLAG_004123"
     CDS             9161..10489
                     /gene="rutG"
                     /locus_tag="ACHLAG_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFP0657253.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNLLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(10572..10745)
                     /gene="ymdF"
                     /locus_tag="ACHLAG_004124"
     CDS             complement(10572..10745)
                     /gene="ymdF"
                     /locus_tag="ACHLAG_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP0657254.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
BASE COUNT         2685 a         3096 c         3268 g         2875 t
ORIGIN      
        1 attcattttt aagcttgcta cgcatgtcac atttaacatg gttgcacaaa gttgcaacat
       61 catggatatt tcacgataac gttaagttgc acctttcaga acaacaggag taatggcatg
      121 ggaaccacca ccatgggggt taagctggac gacgcgacgc gtgagcgtat taagtctgcc
      181 gcgacacgta tcgatcgcac accacactgg ttaattaagc aggcgatttt ttcttatctc
      241 gaacaactgg aaaacagcga tactctgccg gagctacctg cgctgctttc tggcgctgcc
      301 aatgagagcg atgaagcacc gactccggca gaggaaccac accaaccttt cctcgacttt
      361 gccgagcaaa tattgcccca gtcggtttcc cgcgccgcga tcaccgcggc ctatcgccgc
      421 ccggaaaccg aagcggtttc tatgctgttg gaacaagctc gcctgccgca gccagttgct
      481 gaacaggcgc acaaactggc gtatcaactg gccgataaac tgcgtaatca aaaaaatgcc
      541 agtggtcgcg caggtatggt ccaggggtta ttgcaggagt tttcgctgtc atcgcaggaa
      601 ggcgtggcgc tgatgtgtct ggcagaagcg ttgttgcgta ttcccgacaa agccacccgc
      661 gacgcgttaa ttcgcgacaa aatcagcaac ggtaactggc agtcacacat tggtcgtagc
      721 ccgtccctgt ttgttaatgc cgctacctgg gggctgctgt ttactggcaa actggtttcc
      781 acccataacg aagccagcct ctcccgctcg ctgaaccgca ttatcggtaa aagcggtgaa
      841 ccgctgatcc gcaaaggtgt ggatatggcg atgcgcctga tgggcgagca gttcgtcact
      901 ggcgaaacca tcgcggaagc gttagccaat gcccgcaagc tggaagagaa aggtttccgt
      961 tactcttacg atatgctggg cgaagccgcg ctgaccgccg cagatgcaca ggcgtatatg
     1021 gtttcctatc agcaggcgat tcacgccatc ggtaaagcgt ctaacggtcg tggcatctat
     1081 gaaggtccgg gcatttcaat caaactgtca gcgctgcatc cgcgctacag ccgcgcccag
     1141 tatgaccggg taatggaaga gctttaccca cgtctgaaat cactcaccct gctggcgcgt
     1201 cagtatgata ttggtatcaa cattgacgcc gaagaggccg atcgtctgga gatctccctc
     1261 gatctgctgg aaaaactctg tttcgagcct gagctggcag gctggaacgg tatcggtttt
     1321 gttattcagg cttatcaaaa acgctgccca ctggtgattg attacctgat tgatctcgcc
     1381 acccgcagcc gtcgccgtct gatgattcgc ctggtgaaag gcgcgtactg ggatagtgaa
     1441 atcaagcgtg cgcagatgga cggccttgaa ggctatccgg tttatacccg caaggtgtat
     1501 accgacgttt catatctcgc ctgtgcgaaa aagctgctgg cggtgccgaa tctaatctac
     1561 ccgcagttcg cgacgcacaa cgcccatacg ctggcagcga tttatcaact ggcaggtcag
     1621 aactactatc cgggtcagta cgagttccag tgcctgcatg gtatgggcga gccactgtat
     1681 gagcaggtca ccgggaaagt tgccgacggc aaacttaacc gtccgtgtcg tatttatgct
     1741 ccggtcggta cccatgaaac gctgttggcg tatctggtgc gtcgcctgct ggaaaacggt
     1801 gctaacacct cgtttgttaa ccgtattgcc gacacctctt tgccactgga tgaactggtc
     1861 gccgatccgg tcactgctgt agaaaaactg gcgcaacagg aagggcaaac tggattaccg
     1921 catccgaaaa ttcccctgcc gcgcgatctt tacggtcacg ggcgcgacaa ctcggcaggg
     1981 ctggatctcg ctaacgaaca ccggctggcc tcgctctctt ctgccctgct caatagtgca
     2041 ctgcaaaaat ggcaggcctt gccaatgctg gaacaaccgg tagcggcagg tgagatgtcg
     2101 cccgttatta accctgcgga accgaaagat attgtgggct ttgtgcgtga agccacgccg
     2161 cgtgaggtag aacaggcgct ggaaagtgcg gttaataacg cgccaatctg gtttgccact
     2221 cctccggttg aacgcgcggc gattttgcat cgcgctgccg tgctaatgga aagccagatg
     2281 cagcaactga ttggtattct ggtgcgtgaa gccggaaaaa ccttcagtaa cgccattgcc
     2341 gaagtgcgcg aagcggtcga ttttctccac tactatgccg gacaggtgcg ggatgatttc
     2401 gctaacgaaa cccaccgtcc attagggcct gtggtgtgta tcagtccgtg gaacttcccg
     2461 ctggctattt tcaccgggca gattgctgcc gcactggcgg caggtaacag cgtgctggca
     2521 aaaccggcag aacaaacgcc gctgattgcc gcgcaaggga tcgccatttt gctggaagcg
     2581 ggtgtaccgc caggcgtggt gcagttgctg ccaggtcagg gggaaaccgt gggcgcgcaa
     2641 ctgacgggtg atgatcgcgt acgcggggtg atgtttaccg gttcaaccga agtcgctacg
     2701 ttactgcagc gcaatatcgc cagccgcctg gacgctcagg gtcgccctat tccgctcatc
     2761 gctgaaaccg gcggcatgaa cgcgatgatt gtcgattctt cagcactgac cgaacaggtc
     2821 gtcgtggatg tactggcctc ggcgttcgac agtgcgggtc agcgttgttc ggcgctgcgc
     2881 gtgctgtgcc tgcaagatga gattgccgac cacacgttga aaatgctgcg cggcgcaatg
     2941 gccgaatgcc ggatgggtaa tccgggtcgc ctgaccaccg atatcggtcc ggtgattgat
     3001 agcgaagcga aagccaatat tgagcgccat attcagacca tgcgtagcaa aggccgtccg
     3061 gtgttccagg cggtgcggga aaacagcgaa gatgcccgtg aatggcaaag cggcaccttt
     3121 gtcgccccga cgctgatcga actggatgac tttgccgaat tgcaaaaaga ggtctttggt
     3181 ccggtgctgc atgtggtgcg ttacaaccgt aaccagctac cagagctgat cgagcagatt
     3241 aacgcttccg gctatggtct gacgcttggt gtccatacgc gtattgatga aaccatcgcc
     3301 caggtcactg gctcggccca tgttggtaac ctgtatgtta accgtaatat ggtgggcgca
     3361 gtggtgggtg tgcagccatt cggcggcgaa gggttgtccg gtaccgggcc gaaagcaggc
     3421 ggtccgctct atctctaccg tctgctggcg aatcgcccgg aaagtgcgct ggcagtgacg
     3481 ctcgcgcgtc aggatgcaga gtatccggtc gatgcacagt tgaaagccgc attgactcag
     3541 ccgctaaatg cactgcggga atgggcagca aatcgtccag aattgcaggc gttatgtacg
     3601 caatatggcg agctggcgca ggcaggaaca caacgattgc tgccggggcc gacgggtgaa
     3661 cgcaacacct ggacgctgct gccgcgtgag cgcgtgttgt gtattgccga tgatgagcag
     3721 gatgcgctga ctcagctcgc cgccgtgctg gcggtgggca gccaggtact gtggccggat
     3781 gacgcgctgc atcgtcagct agtaaaggca ttgccatcgg cagtcagcga acgtattcaa
     3841 ctggcgaaag cggaaaatat aaccgctcaa ccctttgatg cggtgatctt ccacggtgat
     3901 tcggatcagc ttcgcgcatt gtgtgaagcc gttgctgcgc gggatggcgc aattgtttcg
     3961 gtgcagggtt ttgcccgtgg cgaaagcaat atccttctgg aacggctgta tatcgaacgt
     4021 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
     4081 acaggtagcc ggaggatgtt acaacctcct ccggcatctt taacgtggtc gaatcccctc
     4141 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
     4201 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
     4261 aatcatgaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
     4321 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
     4381 taacagcggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
     4441 gcgggagact tccagcttca gacggatgta ctcttttatc gccgccagcg gagcgaaatc
     4501 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
     4561 atacagcgcc tcttttgacg gaaagtaata cagcagattg gtttttgaaa cacccgccaa
     4621 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
     4681 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
     4741 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggcaacagga tagcaaaacc
     4801 cccgcccggc ctcgaccttc tgcactctca tcgcgcacag atgcatgttt tatgtgcaac
     4861 tgttttgacc gtttagtcca ctttttacca gatattttag acaacacaat gttaacacat
     4921 taataacatt cactttttaa aaactggcat ccgctttgca aacaagccat tacgcggctg
     4981 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     5041 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     5101 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgtgcaa aaagcggagc
     5161 actaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     5221 agttctggga tcacaacctt gagtcgttca ccttgatggc ggggctggcg gccgtcacct
     5281 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     5341 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     5401 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     5461 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     5521 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     5581 gtgtccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     5641 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacgcccacg gctttcgccc
     5701 cgaccgctgc gcggatgaaa caggccgcag agcaaaccgg gcgcgacgtt ggttcttatg
     5761 tgttgtttat ggtgattgcc gatgaaaccg acgatgccgc tcgcgccaaa tgggaacact
     5821 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     5881 cccgctccgg tactgatacc aacgtccgtc agatggccga tcccacttcg gcggtaaaca
     5941 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     6001 caagcgtgcc tggtgccgaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     6061 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatcta cctgcgctga
     6121 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     6181 gcagcaaagt gcgctgatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     6241 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     6301 cgccgtgacc gcagcgcgag cggcagggat gctgatcatc tggtttcaaa atggctggga
     6361 tgaacagtat gtcgaagctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     6421 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     6481 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     6541 ctacagcggt ttcttcaata caccgctgga cagcattttg cgcagccgtg gaatacgcca
     6601 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     6661 ttttctggag tatttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     6721 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     6781 aacgttctgc gacgcgcttt ctcccacatc ctttgctcgt atcgcttaag gagtttaacg
     6841 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     6901 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     6961 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aattatccgc
     7021 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     7081 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     7141 aaaccggcgc gattctgcat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     7201 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct cacctccccc
     7261 ttatgctgat gcgcccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     7321 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     7381 caccggcaat aatcccgaca cgctggcaga agattacagt atcgcccaga tggcagcgga
     7441 actgcatcag gcgctggtag ccgcagggat tgagcattac gcggtggtcg gccatgcgct
     7501 cggtgcgctg gtgggaatgc agctggcgct ggattatccc gcgtcggtaa ctgtgctgat
     7561 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     7621 acgattactg tatagcggcg gcgcgcaggc atgggtggaa gcgcagccgt tgttcctcta
     7681 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     7741 ggcgcatttt cagggcaaaa ataatttact gcgtcgactt aacgccctca aacgcgctga
     7801 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     7861 tctgctggtg ccatcagcat gttccagtga gcttcatgcc gccctgcccg atagccagaa
     7921 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     7981 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     8041 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ttgttcaccg atgcccgcac
     8101 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacgggaga tttatgccct
     8161 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     8221 ggcagaagga aaagaacgtc tgcgcccggc actttccagc ggcaatctgc aaaaaaccct
     8281 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccgca
     8341 actgtttcct cacggcgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     8401 aacagcgttt cgcaacagtt ccatgcaggc ggcctatctg atcgtcgcct gccgtgcgct
     8461 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     8521 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     8581 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     8641 aaaccatgaa cattgtcgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     8701 cagtcaatat catcaccacg gatggtccag ccgggcgcgc cgggttcacc gccagcgcgg
     8761 tctgcagcgt gaccgacacg ccgcctacat tactggtgtg cctgaatcgt ggggcgtccg
     8821 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     8881 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     8941 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     9001 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     9061 cgattcatcg tcacgccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     9121 tgatgcgccc tgcttgttaa cctcctaaag gagacagctg atggcaatgt tcggttttcc
     9181 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgt atgaacgatt
     9241 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     9301 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     9361 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggttccag
     9421 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     9481 gaatatcagc attgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     9541 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     9601 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     9661 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     9721 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggtttaa ttgtcgcctg
     9781 tctgctgtat ggcgtgatga ccaacctttt agggctggga aaagccgtcg attttactct
     9841 cgtcagccat gctgcctggt tcggtttgcc acatttttcc actcctgctt ttaatagtca
     9901 ggcaatgatg ctgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     9961 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gggcattcgt
    10021 cggtgatggg ctggcgacga tgctttctgg ttctgtcggt ggcagcggcg taaccaccta
    10081 cgccgagaat atcggtgtaa tggccgtgac aaaagtctac tcgacgctgg tgtttgtcgc
    10141 tgctgccgtc atcgccatgc tgttgggctt ttcaccgaaa tttggcgcgc tgattcatac
    10201 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
    10261 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacggta atttgattat
    10321 ggtcgcagta accttagtgc tgggcgcggg tgattttgct ctcacgctgg gcggttttac
    10381 gttgggaggg attggtacag caacctttgg cgcgatttta ctcaatgcgt tgttaagcag
    10441 aaagttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
    10501 ccgggattac cggcggtgat gactttttga cgagcctaag aaaaaaatgc attcgtcatt
    10561 gattagcccg gctagttgtc gcttttgccg tgactgctct tgccaccttt tttacctgct
    10621 tcagatgcgc gctgcgggtc attcttgaaa ttacccccgc tgtgctgtcc acctttttta
    10681 cctgcttctg atgctctttc gcggtcttct gcaaaattgc cggaaccgcc tcgatggttt
    10741 gccattactg acctccgtct gggtatagac atcatattgc atagaattaa cgcggcgtat
    10801 ggcggctgcc atattttcgc tacgtgggac taagcttagt gaactgacgc gggcagaccc
    10861 gcgtttggta atatcctgca acagcttctt agcgagaata gatatcatct caaataatta
    10921 tccataagcc gctcttatga ttaaaaaaac gcgatgagat aaaaatgtgt cattttgcga
    10981 caaaattacg tgcttgtgaa agttgttata aatcaaataa gtggttgtga aatttgcact
    11041 ctgaaaagga cgtcttatct ttaaataagt ggtagcgaat cgctacggaa tagagataac
    11101 acgaggagtg gttagaaatg gctaaagttc tggtgctttt acatagctaa acacgtacat
    11161 tcatctacct gtaaaaacag gacaatatca ggtgaatctc tattcaattt aaaggcattt
    11221 tcccaagtat ctatgcaatt ctacacagat tctatgcagt atcgatatag tctcttttta
    11281 gcgtaaccta ttgaaatata tagagaatct atgaagtctc ttttcagggt aaactgtatc
    11341 gtgttagtcg tccctctcca gacactacat atagtgctat atacagggct atataacgat
    11401 atatagatag ttggtcacca tcaatattag attcgattag aagctaatct catattccct
    11461 caaagtgact atcaagctaa ttaatagttt cctatgtgaa agttaggaag tgatggttat
    11521 agttgattcg gttgttctag gaatcaacct catagttcct tcgtagttcc tcttaaaaaa
    11581 tcacttacag acaccttaat atctatacag atttcatgta gaacacgaat agtgcgtaaa
    11641 acagctatta tgttaaaaac gcactgtata cgtatcagta ctgatttata agggttttgt
    11701 gtattggctg ataagaggct atacagcacc ttgagagcat gtcttctaag tcattgacat
    11761 ataaggttac agaagtttgt agctgttaca gagaaaactg ggcaggactt cacagagcta
    11821 taaaatgtta cagctattga taggtgaaat cttcttgata gatgctttga agtgtgatga
    11881 aggtttcttg aggtgggggt aaacagatat catgaagaaa gtca
//
LOCUS       DAZGVQ010000153        11922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000153 DAZGVQ010000000
VERSION     DAZGVQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.154"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..122
                     /locus_tag="ACHLAG_004125"
     CDS             <1..122
                     /locus_tag="ACHLAG_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657255.1"
                     /translation="MTLDEINNTYCNNDYYNQAIREEPIDLLDRSFSSSSWPF"
     gene            446..1834
                     /gene="narU"
                     /locus_tag="ACHLAG_004126"
     CDS             446..1834
                     /gene="narU"
                     /locus_tag="ACHLAG_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFP0657256.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1916..5656
                     /gene="narZ"
                     /locus_tag="ACHLAG_004127"
     CDS             1916..5656
                     /gene="narZ"
                     /locus_tag="ACHLAG_004127"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFP0657257.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5653..7197
                     /gene="narH"
                     /locus_tag="ACHLAG_004128"
     CDS             5653..7197
                     /gene="narH"
                     /locus_tag="ACHLAG_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP0657258.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7197..7892
                     /gene="narW"
                     /locus_tag="ACHLAG_004129"
     CDS             7197..7892
                     /gene="narW"
                     /locus_tag="ACHLAG_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP0657259.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSILPDDQAKEGLLNVAPILALLGGRLKQRKAPWYALFDALLQLAGS
                     TLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGEK"
     gene            7889..8569
                     /gene="narI"
                     /locus_tag="ACHLAG_004130"
     CDS             7889..8569
                     /gene="narI"
                     /locus_tag="ACHLAG_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP0657260.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8648..9541
                     /gene="yddE"
                     /locus_tag="ACHLAG_004131"
     CDS             8648..9541
                     /gene="yddE"
                     /locus_tag="ACHLAG_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFP0657261.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9637..10482)
                     /gene="nhoA"
                     /locus_tag="ACHLAG_004132"
     CDS             complement(9637..10482)
                     /gene="nhoA"
                     /locus_tag="ACHLAG_004132"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFP0657262.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10655..11224
                     /gene="yddH"
                     /locus_tag="ACHLAG_004133"
     CDS             10655..11224
                     /gene="yddH"
                     /locus_tag="ACHLAG_004133"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFP0657263.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11228..11455)
                     /gene="pptA"
                     /locus_tag="ACHLAG_004134"
     CDS             complement(11228..11455)
                     /gene="pptA"
                     /locus_tag="ACHLAG_004134"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFP0657264.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11554..>11922)
                     /locus_tag="ACHLAG_004135"
     CDS             complement(11554..>11922)
                     /locus_tag="ACHLAG_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFP0657265.1"
                     /translation="LKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIR
                     NHWHVENKLHWRLDVVMNEDDCKIRKGNAAELFSGIRHIAINILTNDKVFKAGLRRKM
                     RKAAMDRNYLASVLAGRRLS"
BASE COUNT         2818 a         3082 c         3104 g         2918 t
ORIGIN      
        1 acatgacctt agatgaaata aacaatactt actgtaataa cgattattac aatcaggcaa
       61 taagagagga accgatagac cttctggaca gatcgttttc ctccagttca tggccttttt
      121 agtgaataaa tcatcactaa ttctataagt gtctggtctt gttgataata tagttaatat
      181 gtaacccttt agattcacga tctgaagggt tgaaacatga catacgttca aagattaacc
      241 cccgtttgaa ggttagcttg ttagttacat ttagtaacac atatctggct acttaatccc
      301 cattctgccc ccgcggttac gctttcttat ctattctttc aatcagcact tcacccaatt
      361 catccttaat tctaataact ggctattgcg ccttacctcc tgttttgtct tttttcatta
      421 ccaatgtgtg catgtgagga acaatatggc actgcaaaat gagaaaaata gtcgttatct
      481 tttacgcgac tggaaaccag aaaatccggc cttctgggaa aataaaggaa aacatattgc
      541 tcgaagaaac ctctggatat cagtcagttg tctacttctt gccttctgtg tctggatgct
      601 atttagcgca gttaccgtta atctcaataa aatcggtttt aattttacta ccgatcaact
      661 ctttttatta accgcattac cctccgtttc tggcgcatta ttgcgtgttc cctactcctt
      721 tatggtgcct atattcggtg gacgccgatg gacggttttt agtactgcaa tcctgattat
      781 tccttgcgtc tggctcggaa ttgccgtgca aaatccgaat actccttttg ggatatttat
      841 cgttatcgct ttgctatgcg gttttgccgg tgcaaacttt gcttcgagca tgggcaatat
      901 cagtttcttc tttccaaaag ccaaacaagg gagcgctctt gggattaatg gcggattagg
      961 aaacttaggt gtaagtgtaa tgcagctggt tgcaccgctg gtcatttttg tacctgtatt
     1021 tgcctttctc ggcgtcaatg gcgtaccgca ggccgacggt tcggtgatgt cgctggcgaa
     1081 tgccgcatgg atttgggtac cgctactggc gattgccacg atcgccgcat ggtcagggat
     1141 gaatgatatc gccagttcac gcgcctcaat tgccgaccag ctccctgtct tacaacgcct
     1201 gcatctctgg ctgctgagcc tgctttacct tgccaccttc ggttcgttta tcggtttttc
     1261 tgcgggtttt gccatgctgg caaaaaccca gttcccggat gtgaatattc tgcgcctggc
     1321 gttctttggc ccatttatcg gtgccatcgc gcggtcggtt ggtggtgcta tttccgataa
     1381 gttcggcggc gtgcgggtga cgttgatcaa ctttattttt atggcgattt tcagtgccct
     1441 gctgttcctt accttaccgg gcacaggctc cggtaatttc atcgcctttt acgccgtatt
     1501 tatggggctg tttctgactg cgggtctggg aagtggttct actttccaga tgatcgccgt
     1561 catctttcgc cagataacca tttatcgggt aaagatgaaa ggcggtagtg atgagcaagc
     1621 tcagaaagaa gccgtcaccg aaacggcggc ggctctgggc tttatctcag ccattggcgc
     1681 agtgggcggc ttttttattc cgcaggcgtt tggcatgtcg ctcaatatga ccggctctcc
     1741 ggtcggcgcg atgaaagtgt ttttaatctt ctacatcgtt tgtgtgctgc tgacctggct
     1801 ggtttatggt cggcggaagt tcagccaaaa ataagagatc gatgcctgat gtacaaccgt
     1861 gtcgcatccg acaaggcatc agatcccaat tcattcctgg agcaggagtc atgccatgag
     1921 taaacttttg gatcgctttc gctacttcaa acaaaagggc gaaacctttg ccgatggtca
     1981 cggacaggtg atgcatagca accgcgactg ggaggacagc tatcggcagc gttggcagtt
     2041 cgacaaaatc gtgcgttcca cccacggtgt taactgtaca ggctcctgta gctggaaaat
     2101 ctacgttaaa aatggtctgg tgacctggga aatccaacag accgactacc cgcgcactcg
     2161 ccctgacctg cccaatcatg aacctcgcgg ctgcccgcgt ggcgcaagtt actcctggta
     2221 tctttacagc gctaaccgcc tgaaataccc gctcattcgt aaacgactga ttgaactgtg
     2281 gcgcgaagcc ctcaagcaac acagcgatcc ggtactggcg tgggcatcga ttatgaacga
     2341 tccgcaaaag tgcctgagct acaaacaagt gcgtgggcgc ggcgggttta tccgctccaa
     2401 ctggcaggaa ctaaaccagc tgattgccgc cgctaacgtc tggaccatca aaacctacgg
     2461 cccggatcgc gttgccggtt tctcgccgat cccggcgatg tcgatggttt cttacgccgc
     2521 cggaacgcgt tatctgtcgc tgcttggcgg cacctgttta agtttctacg actggtattg
     2581 cgacctgccg cccgcctcgc cgatgacctg gggcgagcaa accgacgtac cggaatctgc
     2641 cgactggtat aactccagct acatcatcgc ctgggggtct aacgtaccgc agacacgtac
     2701 gccggacgcc cacttcttta ccgaagtacg ctacaaaggc actaaaacca tcgccattac
     2761 ccctgactac tctgaagtgg ccaaattgtg cgaccagtgg ctggcaccga aacaaggcac
     2821 tgatagcgcc ctggcgatgg caatgggcca tgtgatttta aaagagtttc atctcgataa
     2881 tcccagcgac tactttatca actactgccg ccgctacagc gacatgccga tgctggtaat
     2941 gctggagcct cgcgacgatg gtagctacgt tcccgggcgc atgatccgcg catctgacct
     3001 ggtggatgga ctgggcgaaa gcaacaatcc gcagtggaaa accgtagcag ttaataccgc
     3061 aggtgaattg gtagtgccga acggttcgat tggtttccgc tggggagaaa aaggcaaatg
     3121 gaatctggaa tccattgccg ccggtacgga aaccgaattg tcgttaaccc tgctcggtca
     3181 acatgacgct gttgcaggcg tggccttccc ctactttggc ggcattgaaa atccgcattt
     3241 tcgcagcgta aaacacaatc cggtgctggt gcgccaattg cccgttaaaa acctgacgtt
     3301 agtcgatggc aacacctgtc cggtggtcag cgtttatgat ttggtactgg cgaattacgg
     3361 cctcgatcgc gggctggaag atgaaaacag tgcgaaagat tacgctgaaa tcaaaccgta
     3421 caccccagcc tggggtgagc aaattaccgg cgtgccgcgc cagtatattg aaaccatcgc
     3481 ccgtgaattt gccgataccg cccataaaac gcatgggcgc tcgatgatta tcctcggcgc
     3541 aggtgttaac cactggtatc acatggacat gaactaccgc gggatgatca atatgctgat
     3601 cttctgcggt tgtgtcgggc aaagcggtgg cggctgggca cactatgtcg gccaggaaaa
     3661 actgcgccca caaaccggct ggttgccgct ggcctttgcg ctcgactgga accgaccacc
     3721 acgccagatg aacagtacct cgtttttcta caatcattcc agccaatggc gctatgaaaa
     3781 agtctctgct caggagttac tttcaccgct cgccgatgcc agtaagtaca gcggtcatct
     3841 gattgatttc aacgttcgcg ccgaacgtat gggctggcta ccttctgcgc cgcagctggg
     3901 gcgtaacccg ctcgggatta aagctgaagc cgacaaggcc ggattatccc ccacagaatt
     3961 taccgcccag gcgctgaaat cgggcgattt acgtatggcc tgcgaacaac cagatagcgg
     4021 cagcaatcat ccgcgtaatt tgtttgtctg gcgctctaac ctgcttggct cctccggcaa
     4081 aggccacgag tatatgcaga agtatctgct ggggaccgaa agcgggattc agggcgagga
     4141 actcggtgcc agcgacggga tcaaaccgga agaagtcgag tggcaaactg cggcgattga
     4201 aggcaagctc gacctgctgg tgacgctcga cttccgcatg tccagtacct gcctgttctc
     4261 cgatatcgtt ctgcccaccg ccacctggta cgaaaaagac gatatgaaca cctcggatat
     4321 gcatccgttt attcatccgc tttctgcggc ggtcgatccg gcgtgggaat cacgcagcga
     4381 ctgggaaatc tacaaaggta tcgccaaagc attttcgcaa gtgtgcgtgg gtcatcttgg
     4441 caaagaaacc gacgtggtat tacaacccct actgcatgac tctccggcag agctctcaca
     4501 gccgtgtgaa gtgctcgact ggcgcaaagg cgaatgcgat ctgattccag gcaaaaccgc
     4561 gccgaatatt gtggcggtgg agcgcgacta ccctgctacg tatgaacgct ttacctcgct
     4621 cgggccattg atggacaaac ttggcaatgg cggtaaaggg atttcgtgga atacccagga
     4681 tgaaatcgat ttcctcggca aactcaatta caccaagcgt gatggcccgg cacaggggcg
     4741 tccactgatt gacaccgcta ttgatgcatc agaggtcatt ctggcactgg ctccagaaac
     4801 caacggtcat gttgcagtca aagcgtggca ggcgctgggc gaaatcaccg gacgcgaaca
     4861 tacccatctg gcgctgcaca aagaggacga gaagattcgc tttcgcgata ttcaggcgca
     4921 gccacgtaaa attatctcca gccccacatg gtccggtctg gaaagcgatc atgtttccta
     4981 taacgcggga tacaccaacg ttcatgagtt aattccgtgg cgcacgctgt cgggacgcca
     5041 gcaactctat caggatcatc cgtggatgcg agcttttggt gaaagcctgg tggcttatcg
     5101 cccgcctatt gacacccgta gcgtcagtga aatgcgccaa atcccgccaa acggcttccc
     5161 ggaaaaagca cttaacttcc tgacgccgca ccagaaatgg ggcattcact caacctacag
     5221 tgaaaacctg ctaatgctga cgctttctcg cggtggaccg attgtctgga tcagcgaaac
     5281 cgatgcccga gaattgacca ttgtcgataa cgactgggtg gaagtgttta acgccaatgg
     5341 cgcgctgacg gcccgcgcgg tggtcagcca acgtgtaccg ccgggtatga ccatgatgta
     5401 tcacgcccag gaacgcatta tgaatatccc tggttcggaa gtaactggca tgcgcggcgg
     5461 cattcataac tcggttaccc gcgtttgccc gaaaccaacg catatgattg gcggttacgc
     5521 gcagctggcc tggggcttta actactacgg caccgtcggc tcaaaccgtg atgaattcat
     5581 catgatccgc aagatgaaga acgttaactg gctggatgat gaaggtcgcg atcaggtaca
     5641 ggaggcgaaa aaatgaaaat acgttcgcaa gtcggcatgg tgcttaacct cgataaatgt
     5701 atcggctgcc atacctgttc ggtgacctgt aaaaacgtct ggaccggacg cgaaggcatg
     5761 gagtacgcat ggtttaacaa cgtcgaaacc aaaccgggca ttggttatcc gaaaaactgg
     5821 gaagatcagg aagagtggca aggcggctgg gtccgcgatg tgaatggcaa gatacgcccg
     5881 cgtctgggca gtaagatggg cgtaataacc aaaatcttcg ccaacccggt ggtgccgcag
     5941 attgatgatt actacgaacc tttcaccttc gactacgaac atttgcatag cgcaccggaa
     6001 ggcaaacata tccctactgc tcgcccgcgt tcgctgattg acggtaagcg gatggacaaa
     6061 gtgatctggg ggccaaactg ggaagaactg ctgggcggcg agtttgaaaa acgtgcccgc
     6121 gaccgcaact tcgaggccat gcaaaaggag atgtacgggc agtttgaaaa caccttcatg
     6181 atgtacctgc cgcgcctgtg cgaacactgc ctcaatccca gctgcgtggc gacctgccca
     6241 agcggcgcta tctacaaacg cgaagaagac ggcattgtgc tgattgatca ggataaatgc
     6301 cgtggctggc gtttgtgcat tagcggttgt ccgtacaaaa aaatctactt caactggaaa
     6361 agcggcaagt cagaaaaatg tatcttctgt tacccacgaa ttgagtccgg tcaaccgacc
     6421 gtttgctcag aaacctgcgt gggtcgcatc cggtatctgg gcgtgctgct ttacgacgcc
     6481 gaccgcattg aggaagcggc aagcaccgag cgcgaggttg acctctatga acgccagtgc
     6541 gaagtgttcc tcgatccgca cgatccctca gtgatcgagg aagccctgaa acaaggtatt
     6601 ccacaaaatg tgattgacgc tgcccagcgt tcgcctgtct acaaaatggc gatggactgg
     6661 aaactggcgc taccgttgca ccctgaatac cgcaccctgc cgatggtctg gtacgttcct
     6721 ccgctgtcac cgattcagtc ttacgctgat gcgggcggtt tgccgaaaag cgaaggcgtg
     6781 ctccccgcca tcgaaagcct gcgtattccg gtgcaatatc tcgccaatat gttgagtgcc
     6841 ggtgatactg gtccggtact gcgggcgctg aaacggatga tggcgatgcg ccactatatg
     6901 cgttcacaaa ccgtggaagg cgttactgat actcgtgcca tcgacgaagt aggcctgagc
     6961 gtcgcccagg tcgaagaaat gtatcgctat ctcgccattg ccaactatga agatcgtttt
     7021 gttatcccga ccagccatcg ggaaatggcg ggcgatgcct tcgcagaacg caacggctgc
     7081 ggttttacct ttggcgacgg ttgccacggt tcggacagta aattcaacct gttcaacagt
     7141 agccgtatcg atgccatcaa catcaccgaa gtgcgcgaca aagcggaggg cgaataatgc
     7201 agatcctcaa agtgatcggc ctgttgatgg agtatccgga cgagctgttg tgggaatgca
     7261 aggaggacgc gctggcgttg atccgccgcg acgcgccgat gcttacggat ttcacccaca
     7321 acctgcttaa cgcgccgctg ctggataaac aggccgaatg gtgcgaagtg tttgaccgcg
     7381 ggcgcaccac gtcgctgctg ctgttcgaac atgttcatgc cgagtcccgc gatcgcggcc
     7441 aggcaatggt ggacctgctg gcggagtatg aaaaggtcgg cctgcagctg gattgtcggg
     7501 aactgcccga ttatctaccg ctgtatctgg agtatttaag catactgcct gacgatcagg
     7561 cgaaagaagg attgctcaat gttgcgccga tcctcgccct gcttggcggt cgcttaaaac
     7621 aacgcaaggc accgtggtac gcgttgtttg atgctctgct gcaactggca ggaagtacac
     7681 tttcaagtga cagtgtcaca aaacaagtaa acagcgaaga gcgcgatgac acccgccagg
     7741 cgcttgatgc agtgtgggaa gaggaacagg tgaagtttat tgaagataac gccacggcgt
     7801 gtgacagctc gccgcttaat caatatcagc gacgctttag ccaggatgtc gcgccgcagt
     7861 atgtcgacat cagtgcggga ggtgagaaat gattcagtat ctgaacgtct ttttttacga
     7921 tatctacccg tacatttgtg cgacggtgtt tttcctcggc agctggctgc gctacgacta
     7981 cgggcagtac acctggcgcg cctcctcaag tcagatgctc gataaacgcg ggatggtgat
     8041 atggtcgaat ctgttccata tcggcatttt ggggatcttc ttcgggcacc tgttcggcat
     8101 gttaacgccg cactggatgt acgcgtggtt tttgccagtg gcagcgaaac aactaatggc
     8161 gatggtgctc ggtggtattt gcggtgtgtt gacgttgatt ggcggcgcag gactgctgtg
     8221 gcgcaggctg acaaatcagc gcgtgcgtgc cacttccact acgccggata tcatcatcat
     8281 gagcattttg ctgatccagt gtctgttggg gctaagtacc ataccgttct cagcacagta
     8341 tcctgatggt agcgaaatga tgaagctggt gggttgggcg caaagcattg tcactttccg
     8401 tggtggatcg tcagagatgc ttaacggtgt agcgttcgtc ttccgcctgc atctggtgtt
     8461 gggaatgacc atcttcttgc tcttcccgtt cacccgactg gtgcatgtgt ggagcgcccc
     8521 gtttgagtac tttactcgtc ggtatcagat tgtgcgctcg cggcgataat ttcaccctgg
     8581 ccggatagcc cttcgctctc cggccacttg caggctatga tagttctcca gtcacaggag
     8641 aacgttaatg aaaccgcagg tgtaccacgt cgatgctttt acctcacaac cgtttcgcgg
     8701 caattctgcc ggtgttgttt tccccgccga taatcttagc gaagcgcaaa tgcagcttat
     8761 cgcccgcgag ttaggccatt cggaaactgc ttttctgctg cacagcgacg acagcgatgt
     8821 gcgcatccgc tactttacgc caacggttga agtgccgatt tgcggtcacg cgacagtagc
     8881 tgcgcactat gtacgtgcga aggtgttagg tttaggaaat tgcacgatct ggcaaacatc
     8941 gctggcagga aaacatcgcg tgactatcga aaagcacaac gatgattatc gtatttcgct
     9001 ggaacaaggt acgccgggct ttgagccacc gctggaaggt gaaacacgtg aggcgattat
     9061 caacgcgctt catctcactg aagacgatat tttgccgggc ttgccaattc aggtcgcaac
     9121 aacaggacac tcaaaagtga tgatcccgtt gaaaccggaa gtggatatcg acgccctttc
     9181 gcctgatctt gctgcgctga gcgctatcag caaacagatt ggctgcaatg gtttcttccc
     9241 gttccagatc cgcccaggca aaaacgaaac cgatggtcgt atgttctcgc ctgcgattgg
     9301 tattgtggaa gatccggtga ctggcaatgc caatggcccg atgggtgcat ggttggtaca
     9361 tcacaacgta ttgccccacg atggcaacgt gttatgtgtt aaaggccatc aggggcgcgc
     9421 attggggcgt gacggcatga ttgaagtgac ggtgacaatt cgtgataacc aaccggaaaa
     9481 agtcaccatt tcaggcacag cggtgatttt gtttcatgcc gaatgggcaa ttgaactctg
     9541 aaaccgttgc ctgatgcgct tcgcctgtca ggtctacaac cgagctaatg tatgccggat
     9601 aaggcgttta cgccgcatcc ggcaacctga cataaattat tttcccgcct ccgggtgcgt
     9661 atcaaacgcc gccatcacca gcgctaactc atccacggta aagccatgtt tcgcatcatc
     9721 cacgcccaga ccaaactgtt cttgcatcac agcatataat gacgccacat ccggtagatt
     9781 tcgctgctcc accgcgtgcc cattttcata atgggtaaaa tgaaaattgg tcagcgttag
     9841 cttgccaccg tccggcaaat ggcggcacat cagcaaatga tggcgaaaat gcgactgcgg
     9901 ccagtgcgcc gaccagaaat tgcccatcac ataatcgctt tgttgctgct cgcagagatc
     9961 aaaacggtac atcgactgcc agtgctgatg atgattaaac tgcaacaccc aatcatcacc
    10021 ctcctgcaac aaccgataat ctccgtgtgg cgtggtctgc acgagatcgg aaactaaacg
    10081 aatcggcgcg gttagcgtct gcccaccgaa accgacatca gcaatccatt tttcctcttc
    10141 cagttccacc aacagcaaac gatgggtgcg cggcggtaat gctggcggat ttgataacac
    10201 tacgcgccct aacaagctgc gaacgttaaa ctccagctcg cgtaacaccc gctcaaacac
    10261 gccattctgc tcaaaacagt aaccgccacg acgggctatc accagtttct cttccagcga
    10321 ttgatcatca agctgtattt ccctcggcag caaaacgtca aggttttcaa acggaatggt
    10381 gcaattgtgt ttcaggtgca atgcacgaag cgtatcaata ttgaccgcag cagctcccga
    10441 ccagttaata cgggcaaaat agtgattcag aatgggcgtc atcaggcgtt tcccttagtg
    10501 gttttctcaa cttataacct ggaatagacg ccgcaccggc tattttgtgc gatcgcggac
    10561 atgcatcacg tttgcaaatc gccaatcccg ttaatttcgt tacttattgc gttacggact
    10621 acgcttaaaa aaaccttctt cgcgaggcga taccatgagc cgattcatcc ccatagaatt
    10681 acaccatgcc agccgtctat taaatcatgg cccaactgtc atgatcacca gtttcgatga
    10741 gcaatcccag cggcgtaaca ttatggctgc agcctggtca atgccggtgg agtttgaacc
    10801 gccacgcgtg gcgattgtgg tagataaatc gacatggacc agggagttga ttgaacgtaa
    10861 cggtaagttt ggcatcgtta tcccgggcgt tgcagcaact aactggacgt gggcagtggg
    10921 aagcgtgtcg ggtcgtgaag aagataaatt taattgctat ggcattccgg tggtgaaagg
    10981 tccggtgctt ggtttgccac ttgttgaaga gaaatgcctg gcgtggatgg agtgccgcct
    11041 gctacctgcg acttctgcgc aacaaaaata cgacacgctg tttggcgagg tggtatcagc
    11101 agcggcagac gcacgggtat ttgtcgaagg ccgctggcag tttgatgatg ataagctcaa
    11161 tacgttgcat catttaggtg ctggcacgtt tgttactagc ggcaagcgtg ttacggcagg
    11221 ttaagcatta catgctgtaa ccgggtttct ttatcaaagc ctccatttgg ggcgcgattt
    11281 cggcatccca gatagcttgc caagattctg gttgaatctg ctgtagagca atgcttatcg
    11341 aactgtcttt actgttcaga tggcgaataa taacgtcggt aatatctgca gcaagtgctg
    11401 ctttttgttg ttcgtccagt tcacgcggaa agcatttaat gtcgatgtgc ggcatgatta
    11461 cgctcctttt gaaagatgtc agtaaaatta acacacattg tgaatgtcat gtgtccggta
    11521 aagcgtttat acgacaataa tgccagggca agattacgaa agccttctcc ccgcaaggac
    11581 tgacgccagg tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
    11641 gaatacctta tcattcgtca aaatattaat agcaatgtgc cgtatccctg aaaataattc
    11701 tgctgcattt ccttttctta ttttgcagtc gtcttcattc attaccacgt ccagacgcca
    11761 gtgcagctta ttctccacgt gccagtggtt tcggattgct gtggcgaact tctctgcggt
    11821 taaatcagca gaactgatat aatatctgac cgtcatttct ggctctttct tttgttctgc
    11881 tattatggac cgaaaggaga ctgccacgca taatttcttc ag
//
LOCUS       DAZGVQ010000154        11427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000154 DAZGVQ010000000
VERSION     DAZGVQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.155"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            301..1167
                     /gene="folD"
                     /locus_tag="ACHLAG_004136"
     CDS             301..1167
                     /gene="folD"
                     /locus_tag="ACHLAG_004136"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFP0657266.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            1169..1381
                     /gene="ybcJ"
                     /locus_tag="ACHLAG_004137"
     CDS             1169..1381
                     /gene="ybcJ"
                     /locus_tag="ACHLAG_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFP0657267.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1489..2010
                     /locus_tag="ACHLAG_004138"
     CDS             1489..2010
                     /locus_tag="ACHLAG_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0657268.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(2046..3431)
                     /gene="cysS"
                     /locus_tag="ACHLAG_004139"
     CDS             complement(2046..3431)
                     /gene="cysS"
                     /locus_tag="ACHLAG_004139"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP0657269.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3605..4099
                     /gene="ppiB"
                     /locus_tag="ACHLAG_004140"
     CDS             3605..4099
                     /gene="ppiB"
                     /locus_tag="ACHLAG_004140"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFP0657270.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4102..4824
                     /gene="lpxH"
                     /locus_tag="ACHLAG_004141"
     CDS             4102..4824
                     /gene="lpxH"
                     /locus_tag="ACHLAG_004141"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFP0657271.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4942..5451
                     /gene="purE"
                     /locus_tag="ACHLAG_004142"
     CDS             4942..5451
                     /gene="purE"
                     /locus_tag="ACHLAG_004142"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFP0657272.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPYHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5448..6515
                     /gene="purK"
                     /locus_tag="ACHLAG_004143"
     CDS             5448..6515
                     /gene="purK"
                     /locus_tag="ACHLAG_004143"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFP0657273.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6652..7545)
                     /locus_tag="ACHLAG_004144"
     CDS             complement(6652..7545)
                     /locus_tag="ACHLAG_004144"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP0657274.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQRLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSIGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7542..8357)
                     /locus_tag="ACHLAG_004145"
     CDS             complement(7542..8357)
                     /locus_tag="ACHLAG_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP0657275.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLTAAWMQRAEETGLFGPLALAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8368..9627)
                     /locus_tag="ACHLAG_004146"
     CDS             complement(8368..9627)
                     /locus_tag="ACHLAG_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP0657276.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9637..11304)
                     /locus_tag="ACHLAG_004147"
     CDS             complement(9637..11304)
                     /locus_tag="ACHLAG_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP0657277.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNVTPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLSDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
BASE COUNT         2554 a         3252 c         3014 g         2607 t
ORIGIN      
        1 caacacaaat atagacctga agcggtaaat taccaggctg aaaattcttt ttatattgtc
       61 aggtatttct taaattatct taatccttaa acaaggaaat aaatcagttc cagatttaca
      121 acgccatcat ggacgaaaaa tgaagctttc aatctcagcg acctcagcga cggtgcgcct
      181 aaccttcgca agaggtcgct tcacgcgata aatctgaaac gaaacctgac agcgcgcccc
      241 gcttctgaca aaataggcgc atccccttcg acctacgtaa cagatggaat cctctctctg
      301 atggcagcaa agattattga cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct
      361 caaaaagttc aggcgcgtat tgcagccgga ctgcgggcac caggactggc cgttgtgctg
      421 gtgggtagta accctgcatc gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa
      481 gtcgggttcg tctcccgctc ttatgacctc ccggaaacca ccagcgaagc ggagctgttg
      541 gagcttatcg atgcgctgaa tgccgacaac accatcgatg gcattctggt tcaactgccg
      601 ttaccggcgg gtattgataa cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg
      661 gacggtttcc atccttataa cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc
      721 tgcaccccgc gcggtatcgt cacgctgctt gagcgttaca acattgatac cttcggcctc
      781 aacgccgtgg tgattggcgc atcgaatatc gtcggtcgtc caatgagcat ggaactgttg
      841 ctcgcaggtt gcaccactac cgtgactcac cgtttcacta aaaatctgcg tcaccacgta
      901 gaaaatgccg atctgttgat cgttgccgtc ggcaagccgg gctttattcc tggtgactgg
      961 atcaaagaag gcgcaattgt gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt
     1021 gtgggcgacg tcgtgtttga agacgcggct aaacgcgcct catacattac gcctgttccc
     1081 ggcggcgttg gcccgatgac ggttgccacg ctgattgaaa acacgctaca ggcgtgcgtt
     1141 gaatatcatg atccacagga tgagtaacat ggcgacattt tctttaggta aacatccgca
     1201 cgttgagctg tgcgacttgc tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa
     1261 aatcgcgatt gccgaaggcc aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg
     1321 caaaatcgtc gccggtcaga cagtgagttt tgcaggtcac agcgtacagg ttgttgcctg
     1381 atccctttcc cggcctgtat caacaggccg gttctctttt cagacttttg ccctctacgc
     1441 cccgttcatc catgctgata gcatcgacat tttgccgaag cgaccatcat gccaaccgtt
     1501 attacgcacg ccgctgttcc cctatgcatt ggtttagggt tgggttcgaa agtcatccct
     1561 ccgcgtctgt tatttgccgg aatcatcctg gcgatgctgc ccgacgccga cgtattgtcg
     1621 tttaaatttg gcgttgctta cggcaatgtt tttggtcatc gcgggtttac ccattcgctg
     1681 gtgtttgcgt ttgttgtccc gctgttatgt gtgctgattg gacgccgatg gttcagggct
     1741 gggctgattc gctgctggct gtttttaacc gtctcgttgc tatcgcacag cttgctggat
     1801 tcggtaacca ctggcggtaa gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt
     1861 ttcgctccct ggcaggtgat taaagtcgcg ccgtttgcat tgtctcgtta caccacgccg
     1921 tacgggcatc aggtgataat ttctgaattg atgtgggtgt ggttgccggg gatggtgctt
     1981 atgggaatgt tgtggtggcg caggcgataa ccggatgcga aaactcgcat ccggcaacag
     2041 cgcaattact tacgacgcca ggtggtccct tgcgggccat cttccagcac gatccccatc
     2101 tcgttaagac ggtcacgcgc cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc
     2161 agacgctgtt gaattaacgc ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg
     2221 ctttgcagga acgcttccgg ttcttgctcc agcaggccca atacagcgga aagtttacgc
     2281 aggtgagatg ccattgcatt cgccgctgcc atatcttctg ctttcagacg gtttacttca
     2341 cgcgccatat caaacagtac ggaataggct tccggggtgt tgaaatcgtc gtccatcgct
     2401 tcaataaagc gcgcttcaaa cgcttcgcca ccggcaggcg caacggtttt atctgtgccg
     2461 cgcagcgcag tgtagagacg ctccagcgcc gcacgcgcct gcttcaggtt ctcttcgctg
     2521 tagttcaact ggctgcgata gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg
     2581 tagtatttca gcacatcgcg cacggtaaag aagttaccca gcgatttgga catcttctcg
     2641 cggtcaacca tcaccatccc cgagtgcatc cagtagttca catactggcc atcatgggca
     2701 caggtggact gcgcgatttc gttctcgtgg tgcgggaaca tcaggtctga accgccgccg
     2761 tggatatcaa agtggttacc cagctgcttg cagttcattg ccgaacattc aatgtgccag
     2821 cctggacggc ccgcgcccca cggagacggc cagctcggtt cgccctcttt cgacatcttc
     2881 cacagaacga agtccatcgg gttgcgtttg tcgtcgacca cgtcaacgcg cgcacctgcc
     2941 tgcagctggt cgagatcctg acgcgacagc acgccatatg tcggatcggt cgggacgtcg
     3001 aacatcacgt cgccgttgtc cgccacataa gcgtgacctt tggcgatcag ttgttcagtg
     3061 agttcaataa tttctgcgat atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg
     3121 ttcaaagcat caaaatcttt gtgcatttcg gcgatcatgc gatccaccag cgccacaaag
     3181 ctttcgccat tttcattggc gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc
     3241 acatacttca gtttatagcc gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca
     3301 aaggtacgcc cgtgaccgat atgacagaga tcgtaaacgg tgattccaca cacatacatg
     3361 ccgacttccc cggcgtgaat aggcttaaat tcctcttttt ggcgtgtcag agtattgaag
     3421 atttttagca tcgaagattc cgtttagaca tgtgtgggta attgagttgc gtataatacc
     3481 catatttccc gcgcgaatca gcatacattg cgtgatgatc cgatcgtgcg gttatgctat
     3541 aacaccaccc tatatatgac ccgaactggg ttgaagcacc aatcaaacgg aacaggatgc
     3601 aaaaatggtt actttccaca ccaatcacgg cgatattgtc atcaaaactt ttgacgataa
     3661 agcacctgaa acagttaaaa acttcctgga ctactgccgc gaaggttttt acaacaacac
     3721 cattttccac cgtgttatca acggctttat gattcagggc ggcggttttg aaccgggcat
     3781 gaaacaaaaa gccaccaaag aaccgatcaa aaacgaagcc aacaacggcc tgaaaaatac
     3841 ccgtggtacg ctggcaatgg cacgtactca ggctccgcac tctgcaactg cacagttctt
     3901 catcaacgtg gttgataacg acttcctgaa cttctctggc gaaagcctgc aaggttgggg
     3961 ctactgcgtg tttgctgaag tggttgacgg catggacgtg gtagacaaaa tcaaaggtgt
     4021 tgcaaccggt cgtagcggta tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag
     4081 cgtgaccgtt agcgagtaat cgtggcgaca ctctttattg cagatcttca tctctgcgtg
     4141 gaagaaccgg cgatcaccgc cggttttctg cgttttttag cgggggaagc ccgcaaggcc
     4201 gacgcgctgt atattcttgg cgatctgttt gaagcatgga ttggcgacga cgatcccaac
     4261 ccactccatc gccagatggc ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt
     4321 tatttcattc atggcaaccg tgattttctg ctcggcaaac gctttgcccg tgaaagcggc
     4381 atgacattat tgccggaaga aaaggtgctc gaactttatg gtcgccgggt gttgattatg
     4441 catggcgaca cgctgtgcac cgatgacgcg ggttatcagg cttttcgcgc caaagtccac
     4501 aaaccctggc tgcaaatgct attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc
     4561 gcgcgaatgc gagcgaacag taaagaagcc aacagcagta aatcgctggc gatcatggac
     4621 gttaaccaaa acgcggtagt cagtgcgatg gaaaaacatc aggtgcaatg gctgatccac
     4681 ggacataccc atcgcccggc ggtacatgaa cttatcgcca accagcaacc tgcttttcgc
     4741 gtggtactcg gtgcctggca tacggaaggt tcgatggtga aagtcacggc ggatgacgtt
     4801 gagctgattc attttccgtt ttaaaaaacc cgcaactttg ctgatttcac agctacgcaa
     4861 ccgttttcct tgctctcttt ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc
     4921 accacaggag ttttaagacg catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg
     4981 atggggtcca aaagcgactg ggctaccatg cagttcgccg ccgaaatctt cgaaatcctg
     5041 aatgtcccgt accacgttga agtggtttct gctcaccgca cccccgataa actgttcagc
     5101 ttcgccgaaa gcgccgaaga gaacggttat caggtgatta ttgcgggcgc aggtggcgca
     5161 gcgcacctgc caggcatgat tgccgccaaa acgctggtgc cggtgctggg cgtgccagta
     5221 cagagcgccg cactgagcgg tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc
     5281 attccggtgg gtacgctggc gattggtaaa gctggcgcgg caaacgcggc attactggca
     5341 gcacaaattc tcgcgactca cgataaagag ctacaccagc gtctgaatga ctggcgcaaa
     5401 gcccagaccg acgaagtgct ggaaaacccg gacccgcgag gtgcggcatg aaacaggttt
     5461 gcgtcctcgg taacgggcag ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca
     5521 ttgctgtctg gccggtcggg ctggacgctg aaccggcggc ggtgcctttt caacaaagcg
     5581 tgattaccgc tgagatcgaa cgctggccgg aaaccgcatt aacccgcgag ctggcgcgtc
     5641 atccggcctt tgtgaaccgc gatgtgttcc cgattattgc tgaccgtctg actcagaagc
     5701 agcttttcga taagctccac ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg
     5761 agtggcctgc ggtgtttgat cgtttaggtg aactggcgat tgttaagcgt cgcactggtg
     5821 gctatgacgg tcgcggtcaa tggcgtttac gcgccaatga aaccgaacag ttaccggcag
     5881 agtgttacgg cgaatgtatt gtcgagcagg gcattaactt ctctggtgaa gtgtcgctgg
     5941 ttggcgcgcg cggctttgat ggcagcaccg tgttttatcc gctgacgcat aacctgcatc
     6001 aggacggtat tttgcgcacc agcgtcgctt ttccgcaggc caacgcacag cagcaggcgc
     6061 aagccgaaga gatgctgtcg gcgattatgc aggagctggg ctatgtgggc gtgatggcga
     6121 tggagtgttt tgtcaccccg caaggtctgc tgatcaacga actggctccg cgtgtgcata
     6181 acagcggtca ctggacacaa aacggtgcca gcatcagcca gtttgagctg catctgcggg
     6241 cgattaccga tctgccgtta ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc
     6301 tgattggtag cgatgcgaat tatgactggc tgaaattgcc gctggtgcat ctgcactggt
     6361 acgacaaaga agtccgtccg gggcgtaaag tggggcatct gaatttgacc gacagcgaca
     6421 catcgcgtct gactgcgacg ctggaagcct taatcccgct gctgccgcca gagtatgcca
     6481 gcggcgtgat ttgggcgcag agtaagttca gttaagcagg tgctcatttg attgccggat
     6541 gcggcgtaaa cgccttatcc ggcttaccgc tccggtaccc attgtaggcc tgataagacg
     6601 cgtcaagcgt cgcatcaggc atggtgcacc gattgccgga tgctctcatc actacagcga
     6661 aatacaggtc cccgcttcgc ccgccagcgt ctcttcaatt cgcgataacg ccccaatcca
     6721 cgcgggttta ctacggcttc tgacataacc actcaccgcc gttaccttcg gcccgatcga
     6781 accatcggct ttggcaaatg gcgctaactc atccggtgtg gcatggcgaa tggcacgttg
     6841 ctgcggcgtt ccccagtttt catataccgc atcagcatcg gtgaggatca ccagtccatc
     6901 ggcattaatc tgctcggcga gcaacgcagc ggcgagatct ttatcaatca ccgcttcact
     6961 ccctgcccca tcttccgtca caggcacacc gccgccgcca ctgcaaatca ccacatgccc
     7021 ctctttgagc aacaactcga tggcttcgct gtcgagaatt ttacgcggtt gcggagacgc
     7081 caccacccgg cgcaagtatt taccatcacg tttcatctgc cagccgtaag ctgcttccag
     7141 tgcctcttgt tcttctggct gataaaccgg accaataaat ttctctggct gcaaaaacgc
     7201 cggatcatca ggcgaaacct caatgcgcgt cagcaccgtc gtaacaggcg gcatctgcgg
     7261 ctgtgcgctc aacctctgcg ccagcatata gccaatcatc ccctggcttt ccgccaccag
     7321 cacatccagc ggatacggtt ctacctcttt ccacgccaga ttctgtaacg ccagcagccc
     7381 cacctgcggc ccgttgccgt gaacaatcgc cagccgataa gaacgggcca ggcgtgccag
     7441 cgcgggtaca gcactggcga tattgcgata ttgattttcc gccgtcagcg cctcaccgcg
     7501 ctggagtaag gcgttgcccc caagagctac aaccagtgtt ttcatggttt tccttgtaat
     7561 aattgttggc caagccagaa ccccagcagc gtatctgccc ctgacgtatg ccccagcgcc
     7621 agcagcgaat caatcgcaac agcggtacgt ttcgggcaac tcagagcatg aacaaagtgc
     7681 aggagtggcg aggcgaaata tccttgcgcg gcataacgta aataactgac gctcaccgct
     7741 gtggtaacga gttgaagatt gtcggaacag gcaaaaaacg ggcgaccaga gcgcgcatct
     7801 aaagcgccat aataccaggc cgccagcagc attccgctca acgtgtcatc atgacttggc
     7861 gttaatccgg gtcctttacc cagccagtgc cgccagtcgg tcttaacgcc attgagcgcg
     7921 gcctgaaaac agtgacgaaa ctggcgtaat tcagcggtca gcggatcgct tgccgccaac
     7981 gccagtggcc cgaaaagccc ggtttcctcc gcgcgttgca tccatgcagc tgtaagcggt
     8041 tgaggatgcg caggcggcgt aatacgcagc aaacaataac gctgtggctg tttaaccgtg
     8101 aaacgcccga ggcgaatccc ttgagccaca acctgtggtc gttcattgcc gcataatcca
     8161 ccgcataacg catcgaattg cgcgcggcga agcacccatc ctccggggcc gaaaccacta
     8221 ccctgacgat gcagcgttaa cagttcgccg ctttccgtca tcaggttaat cgcccgccgc
     8281 cacactcccg ctaaccgcca ggactggcga taattcggtg cactacggct ggcaagcaga
     8341 ggatggatga tcgtcattgc gcgttcctca accaatcccc atgctttcag ccagtgcttc
     8401 cagcgcctgt tcaaaacacg ccagcggtgc ccgcacagtg cctgctccaa tctgcccgat
     8461 ccccgcctct ttatgggcga taccggtatt gatgagtggc gtaatgccgg tttctaccac
     8521 gcgacgaatg tccagtccca ggcacgcgcc ctgaaaatcc cagcctggga tctgcaactg
     8581 catattgcgc tcaaggtaaa tttccgccat ctcttcagat accgctctgg ccgcttccat
     8641 gccacccgca ccgacaaagc gcgttacgcc aggcgctgcg atcattgccg cacctccgat
     8701 accaaaggtt tcggtaatcg cgctatcgcc catatccggg ttcgcctgct cctgcgagaa
     8761 gccggtgaaa aacagacctt gcggagtgtt tacaggcgca gtaaaccagc gttcgcccag
     8821 cccgcttacc cgaatcccga acatattgcc gttgcgggtc attgccgtga cgatgctgcc
     8881 tgcgcggatc atcgcgccag catccatcgc cgccttgcag taagccatcg cgaggttgag
     8941 gaagaactga tcggtcacgc tgaggaaatc catcacttcg gcgatgtgct gtttatcatg
     9001 atcgaggcga gcaatttgtg gggccagcgc acgcattaac agtgcagagg aagcaatatt
     9061 gcgttgatgg aactcatcgc ccatcgtaat gccctgcgcc atcatcgccg tgagatcgat
     9121 accgcgctcc atgcgcccca gcgccgcgct taatactggc attaacacat cgcgcatcca
     9181 gcgatggcga gtcaggacat cttcgccgta agcgccaaaa cgcatcactt tgccgatacc
     9241 ttcgttgagg ttgcagtacg cccggttgcc gtcggtcacg ttctcgacca ccagcatcgg
     9301 catactggca gaagtaatac cgcccattgg accgacggca ttcacatggt gacaaggaat
     9361 gaagttcact tccccctgct ccagtattgc cagcgcctgc gcttcatctt tcgcccagcc
     9421 ttcgaacaga catgcgccca cgcacgcccc tttcatgggt ccggtcatct cctgccagcg
     9481 cattggcggc ccggcgtgaa gcagtgtttt gccctcgttt agttcgctga taagtgaaga
     9541 agccggttgc acatccagcc agtgtggacg agcacgacga atttgttcga taaccgcagc
     9601 attggcttgc gccactgatg taaacatggg aaccccttat tgcaaacgtt ctaataaacg
     9661 agccagtttt ttattgccgc cggcgactgg cgaccattgg taatgcacaa ccggtttgct
     9721 ggcgctttgt agctccagcg caaagctgcg taatccgatg ttaatcacgg cgacgttttc
     9781 cagtaatgac ggtgtgtgtt gctgagtggc aggcgaaagc ggacgaatca gtgccgccgc
     9841 cagcaaggtg gcttccggca gcgaactcac taccgcgatc cctgcatctt ccagcgtggc
     9901 gatttgctgc gagcggcatt gcgggtcacg ttcagtgcct gtcaccgtgg caatggcata
     9961 cagtggttga ttgtctgaac gcgcggcaca tgctttttgc caggcgctta ccagcgaggc
    10021 ggcaggatcg gcggtcgcac cgaagccaat cacgacatca agcagcaata cgcgcacttg
    10081 cggtttagcg ccgagatcgg caattaactg attgcgtaag gctgggtcga tcatcggatg
    10141 gggacgcccg acggtgtaga aatcatcgcc gaggtcaata atctggtgac catcggcgtc
    10201 gagcatcatg ccgtgatgat gggtatcgtc ggcttccacg ccaaggtgcc cggcaagtaa
    10261 tcccgccgct tcggcagcca gcgtaccgcc ggtatacaaa ccgcaaataa atccgctgct
    10321 ggcaggcgtt atcgcgttac gtcgcgccgt aacgcgtgaa agcaggcaag ccaggcgtgc
    10381 ggcctcatcc agcgaggagg caaaccagac attctcgtcg cgggccaccg ccggggtata
    10441 acctaaaaac agcgccaccg tcggtttgcc ggttgctttc atggcattaa caattttcag
    10501 acgcacagct tcggcaggcg gttttgaaac aaatgccagc acttcgcttt tctcgtctgc
    10561 actgagcatt tccagcgccg tcagcgcact gatgccgccc acttcacggc tgaggtcgcg
    10621 cccgccaagg ccaatcgcgt gagtaattcc ctcccctgcc agcgcaatct gtgagcacag
    10681 ctcctgaatc ccggtaccgg aagcgccaat gacgccaatg ttgccttccg gcatcacgtt
    10741 agcaaaagcc agcggcgtgg cggcaatcat cgacgtaccg cagtccggcc ccatcaccag
    10801 caagcctttt tctcgcgcgc gggttttaag ttggatttca tcttccagcg tgacgttatc
    10861 ggagaacatc atcacgttga ggttgcgatc cagcgcctga ttcgccagct ccgccgcata
    10921 ctcgccagcc actgaaatca gcgccagatt ggcatcggat aatttctgac aggcactgtc
    10981 ccagcgacgc acctgcgtca aggactggct gctgcctgac ccctgcgcca gttgttttag
    11041 cgcctcttca agctgctgca taatcgcctg cgcgatcccc gcatccgccg cttcgctacg
    11101 tattgccacg caaatatcgt tcggcgtgac gttattaaaa tcgtcatgcc agaaacctgt
    11161 ggtatctaat aacgctttat tggcgggcgt acccatcatt acggaaacat catcaacatt
    11221 ttctgattcg ctgagttttc gtgaaataat cattaaactg accgaatcct gaaaacaccc
    11281 ttttttaata aaggcgtgga tcataacaac tccttaaaaa ttgcacccat ggcaattgca
    11341 cgagcagtgt taatgtggcg attacggtat cgatgacaac ggataaataa ggtgatgaat
    11401 atcacacagg gattattaac aaaagaa
//
LOCUS       DAZGVQ010000155        11412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000155 DAZGVQ010000000
VERSION     DAZGVQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.156"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            97..597
                     /locus_tag="ACHLAG_004148"
     CDS             97..597
                     /locus_tag="ACHLAG_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFP0657278.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLLGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            689..3373
                     /locus_tag="ACHLAG_004149"
     CDS             689..3373
                     /locus_tag="ACHLAG_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFP0657279.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            3370..4458
                     /locus_tag="ACHLAG_004150"
     CDS             3370..4458
                     /locus_tag="ACHLAG_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFP0657280.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4546..5844)
                     /gene="exuT"
                     /locus_tag="ACHLAG_004151"
     CDS             complement(4546..5844)
                     /gene="exuT"
                     /locus_tag="ACHLAG_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFP0657281.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            6327..7739
                     /gene="uxaC"
                     /locus_tag="ACHLAG_004152"
     CDS             6327..7739
                     /gene="uxaC"
                     /locus_tag="ACHLAG_004152"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP0657282.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            7754..9241
                     /gene="uxaA"
                     /locus_tag="ACHLAG_004153"
     CDS             7754..9241
                     /gene="uxaA"
                     /locus_tag="ACHLAG_004153"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFP0657283.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGVHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9324..9875
                     /locus_tag="ACHLAG_004154"
     CDS             9324..9875
                     /locus_tag="ACHLAG_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFP0657284.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9880..11124)
                     /gene="sstT"
                     /locus_tag="ACHLAG_004155"
     CDS             complement(9880..11124)
                     /gene="sstT"
                     /locus_tag="ACHLAG_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFP0657285.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         3029 a         2756 c         2942 g         2685 t
ORIGIN      
        1 aatcgttgtc aaatataata actggatcac caaagataat ggcgagtttg aactggcggt
       61 ccattaatta tcattgtaat aaacgaagga atagtaatga aaaaggtatt tgcaaaatct
      121 cttctggtcg cagcgatgtt ttctgttgca ggctccgcat tggcagtaca aaaggatatt
      181 actgtaacgg ccaacgttga tgccgctctg gatatgacac agaccgataa caccgcgttg
      241 ccgaaagcag tagaaatgca atatctgctg gggcagggtc tgcaatctta ccagttgatg
      301 accaaaatct ggtctaacga cgttaccaaa gatgtaaaaa tgcagttagt ttccccggcg
      361 caactggttc aaagcctgga tgctagcaag attgttccgc tgacagtaac ctggggcggt
      421 gaagaaatta aagctgatgc ggcaaccact tttaccgcaa ccaaaatctt tgcttccgat
      481 gcgttaacta acggttctct ggctaaaaac ctgatgtttg ctcaaaccac caaaggtgtt
      541 ctggagacag gcatctaccg tggtgtagta agcatttatc tgtctcagga tatctaatcc
      601 agataacaga attaacacgt aacaaaggga ggggtagccc tcccttattt taactgtttt
      661 agtatttgtt atggatttca ggattgccat ggataaaaaa ttactggctc ttttgatcct
      721 ggcgagtctc agcccggcag aggcgacatt aaccaaaatt cccgcagggt ttgaggttat
      781 tgctcaggga cagcaggagt atatcgaggt ttatttttca gggaaaagtc tcggtaaata
      841 ttatgcaatg gttaatcttg ataccgtaac atttcttgat ccagcaagtc tatataacaa
      901 gctggaactg gatgtagacg atcagaaaat cgcgcatata gtgaaagaaa aattatcgca
      961 gccgctagct cgccacggtg aattggcttg cggttatgta cgtactgact cagggtgtgg
     1021 ttttctgaat accgatacgc tggaaataat ctataatgat gaagaaagtt cggcaacgtt
     1081 gtttattaat ccgcaatgga attcagcttt cgatgcgaag tcattatatt taaatccaga
     1141 caaaaatact gttaatgctt ttatacatca gcaagacatc aatgttctgg cacaggatga
     1201 ttaccaatcg ttgtctattc agggaaacgg tgcgctggga ataacagaaa atagttatat
     1261 tggtgcacac tggaatttca acggttatga tgcagatgat gtcagtgaca gtaatgctga
     1321 cgtcagcgat ctctattatc gttatgattt tttacgtcgt tattatgtgc aggcgggtcg
     1381 catggacaac cgcacactat ttaatgcaca aggcgggaac tttaccttta actttctgcc
     1441 actcggtgca attgatggga tgcgtatcgg gtcgacactg agctatttaa accaggcgca
     1501 aagccagcaa ggaaccccag taatggttct gctttcgcgc aattctcgtg ttgacgctta
     1561 tcgtaatgag caacttttgg gatcgtttta tctcaatagt ggttcgcaat ttattgatac
     1621 cagttccttt ccgccaggca gctacagcgt ggcattaaaa gtctacgaaa ataaccaact
     1681 cacccgcacc gagcttgtgc cgtttaccaa aaccggcggt ctgactgacg gaaatgcgca
     1741 atggttctta caggcaggta aaactacatc acaggtttct gatgatgaaa gttcagctta
     1801 tcagctagga gtacgcctgc cattacatcc gcaatatgag ctctacgcag ggctggcgaa
     1861 tgccgatgat gtgagtgctt tcgagttagg taataactgg acggcagatt taggcggggt
     1921 ggggaatctt gcaatcagcg ccagcgtgtt ccgtaacgat gacggcggca aaggtgatat
     1981 gcaacaggcc aactggagta atccgggatg gccgacgttg ggcttttatc ggaccaactc
     2041 tgacggcgat gcttgtacaa ccgacagcag agagagctat aacgccttaa gctgttatga
     2101 aagtatttcc gcgacggttt cacagaattt tgtcggctgg aatatgatgc tgggttatac
     2161 ccgcacacaa aataacactg atgatagttt gcgttgggat aaacagcaga gctttgaaaa
     2221 taactatctt cgccagacaa cagcgcaaag tatctctgaa actgtacaac ttagcgcttc
     2281 ccgcgctttt gtgatgcgtg actggatttt gagtacgtca gtcggtgttt tccatcgtaa
     2341 tgacaacggt ggcgataacg acgacaacgg cttgtactta tcgttttcgt tatctgacac
     2401 gccaacgatg gatagcaata acaacagcca ttcaacaaat gtgtctacgg attatcgtta
     2461 tagcgaacag gatggcgatc aaacgtcatg gcagttatcg catacctttt ataacgattc
     2521 attcagccat aaagaacttg gcgtaaccgt cggaggcctg aacaccgata ccataaacag
     2581 cgcggttaac gggcgttggg atgggcaata cggaaatgtc tacgctaccg tatctgatag
     2641 ttatgaccgt aagaatcatg atcatctttc ggcctttacg ggcacttata gctctacgct
     2701 ggctgtcagt cgctatggcg ttaatttggg agccagtggt acagacgatt tgctgggtgc
     2761 ggtactggtg gatgtgaaag gcttctctga acaggatgaa gagagtcagg atctgcaact
     2821 cgaagcgcgg gtggcaggca gccgaacttt gcagcttggt caaagtgaca gtgtgttgtt
     2881 cccttatcct ggatttcagt ctggttttgt tgaggttaac gacagtagcc agggcaatca
     2941 gcaggggaca acaaacatca ttaacggtgc agggaatcgt gaattaatgt tgttgcctgg
     3001 caagctgcgc tatcgcgaag tgtctgccag ctttaactac aactatatcg gtcgtttatt
     3061 actgccggca gcggtgaaaa aattcccgat tgtggggctg aacagcgcca tgttactggt
     3121 agctgaagat ggcggattta cacttgaaat taacggcagc gaaaaagagc tctatctgct
     3181 ttccgggcag caattcctta agtgtccact gagtgttgta aagaaacgcg ccagcattcg
     3241 ttacagcgga gatgttactt gtagtgtggt gacttattca caattaccgg agtcgattca
     3301 ggttcaggca cagttgaaac agcctaaatt acgtggaaac gttcagacgg cgcaaaggga
     3361 ggttgcacca tgagaaatcg attgattgcg gcgatattgg gcttgtttgg cacgctcact
     3421 ggcgttcagg cagctcctga cgtgaccagt gaaattacgt atgatttggc atctggtaga
     3481 gcggattatt acttctggaa ggatgaagcg tcggcaggaa ataatggata tatgtggtat
     3541 gaatgttcgt atcctgacct ccaacaaacc tgtacagcta atggaaatat atcgacagta
     3601 caaatctatt taactgaaca acgcagtggg atgcgttggc cggtaaaact caaaggattt
     3661 aaaacagcca ttgtaagtag cgatgaagcg ccgccaggat gcaagggggg caaagggctt
     3721 cagacgaatc ttaaggattc taatagatct tcatgtacag aagatggtca acattattat
     3781 atatacgata caaagtttct tacgctctac cttgagcaga cagagatgaa gaatttgccg
     3841 attggtggcg tctggaaggg gaaagttaaa ttacattcga acagcccggc ccaggactat
     3901 ttcgcaaata ttaccctgaa tacgctcgac cccaaccata ttgacgtgtt cttcccggag
     3961 ttcgcccacg ccacgccaag ggtgcagtta gacttgcatc caacaggaag cgttaacggc
     4021 agcaactacg cgcaagatct gaccatgttg gacatgtgcc tgtacgatgg ttttaacggt
     4081 aatgccatca gttatgaaat catgctcaaa gatgaagggc gaccagctgc agggcgcaga
     4141 gacggttact tctctatcta tcgtcaggga gggaccacca ccgacgaggg agaacgcatt
     4201 gattaccggg tcaaaatgta caacccggaa accggtgggc aaattgatgt gcgcaataat
     4261 gaaaatatgg tctggaacag cattaacctg aaacgtgtgc gtccggtggt actgcccggt
     4321 attcgctatg ccgtgatgtg tgtgccgaca cccttgactc tggcagtcga taaattcagc
     4381 gtgatggata aacaggccgg gtactacatg ggcaaattgt cagtaatctt tacgccttcc
     4441 ttgccaacca tcaattaacc ctgcaaaccg gacaaacgct tttttctata taaacgacga
     4501 agccgtctga taaggcggct tcgtcattac aacttaagtt aaacgttaat gttgcggtgc
     4561 gggatcgtta tgtgtttcct gcgccacctc aatcgccggt ttgttctgca acacggtcca
     4621 gataaccagc gcacctaaca ggtcaaatac tgccagaact gcgaacagcg ggctgaagcc
     4681 gatggtgtca gccagtgcac cgacaaccag cgcaaacagc gtacttgcca gccatgcgga
     4741 catcccggtt aaaccgttcg ccgtcgccac ttcgttacga ccgaacacgt cagaagagag
     4801 cgtaatcagc gcgccagaca gtgcctggtg ggcaaaacca ccgatacaca gcagcataat
     4861 tgcgacatac gggttggtga acagaccgat catacccggg ccaatcatca gcacagcacc
     4921 cagcgttacg accatcttac gggaaacgat caggttcaca ccaaaccaac gctggaacag
     4981 cggcggcagg taaccaccga ggatacaacc gaggtcagca aacagcatcg gcatccaggc
     5041 gaacatcgcg atctctttca ggttaaagcc gtaaacttta aacatgaaca gcgggatcca
     5101 cgcgttaaaa gtaccccagg ccggttctgc caggaaacgc ggcagcgcga taccccagaa
     5161 ctgacggtta cgcaggatct gaccaacgga cattttcttc gccgtgctca cctggtgctg
     5221 ggcttcctga ccattaataa tatagtcgcg ttcttcatcg gtcagatgct tctggtcgcg
     5281 tggatgttta tagaaaatca gccatgccat cgcccagata aagctcaatg caccggagat
     5341 gataaatgcc atctgccagc tgtgcattac gattgcccat accaccagcg gcggcgcaat
     5401 catcgcacca atcgaagaac ctacgttaaa gtaacctacc gcgatggaac gctctttcgc
     5461 cgggaaccat tcggagctgg ctttcagacc cgccgggatc atcgctgctt ccgcggcacc
     5521 gactgcacca cgagcaatcg ccaggccacc ccagctacca gccagcgcgg ttgcaccaca
     5581 gaacacggcc cacagcacag caaacattgc ataaccgatt ttcgtaccca gcacatccag
     5641 tacatagccc gctaccggtt gcatgaccgt ataagcagca gaataggctg cgatgatata
     5701 ggaatactgt tgggtggaga tgtttaactc ttccatcaga gttggcgcag ctgccgccac
     5761 agtgttacgc gtcaggtaac caagcacggt gccaagcgtc accagtgcga tcatatacca
     5821 acgtaaccct ttaattttac gcatgtaaaa cctcatcgtt ttgttatatc cgcaggaagc
     5881 gcggccatca tcccgccaga ggctgaagat gttagttacg accgggtaac cgaaaaaaaa
     5941 ccggcacgcc ccgaacgttg ccatgaaagt ttcgtgctaa ttcggcttcc gtaccggtta
     6001 acccgattca tctaacaaat gaatgaatcg gcaatttgca ttccgtcgac ttatgatttg
     6061 cgacggcaga aagataactt gtcatacaac tttaaaaggt gagagccatc acaaatgtgc
     6121 gaatatttgt agggacatta cctgacgaca gcgaggccag tactggcgcg gcctgcagag
     6181 agatttacca ctttgagagt aattttttta actacgttta ttgatctaac tcacgaaaat
     6241 atcttcggac tctggaaatt ggtgtgataa ctttgtcagc atcgcaccat aagcaagcta
     6301 gctcactcgt tgagaggaag acgaaaatga ctccgtttat gactgaagat ttcctgttag
     6361 ataccgaatt tgcccgccgt ctgtatcacg actacgcaaa agaccagccg attttcgatt
     6421 accattgcca tttgccgccg cagcagattg cggaagacta tcgttttaaa aacctgtatg
     6481 acatctggct gaaaggcgat cactacaaat ggcgcgctat gcgtaccaac ggtgtggccg
     6541 agcgtctgtg taccggtgat gcgtctgacc gtgaaaaatt tgacgcctgg gcggcgactg
     6601 ttccgcacac tatcggcaac ccgttatacc actggacgca cctcgaactg cgtcgtccgt
     6661 ttggtatcac tggcaaattg ctttctccgt caactgccga tgaaatctgg aacgaatgta
     6721 acgaactgct ggcgcaggat aacttctccg cgcgcggcat catgcagcag atgaacgtga
     6781 aaatggtcgg caccaccgat gacccgatcg attctctgga gcatcacgca gagatcgcca
     6841 aagacggctc tttcaccatc aaagtgctgc cgagctggcg tccggacaaa gccttcaaca
     6901 tcgaacaggc gacctttaac gactacatgg cgaagctggg cgaagtttcc gataccgaca
     6961 ttcgccgctt tgctgacctg caaactgcct tgaccaaacg tctggatcac ttcgccgctc
     7021 acggctgtaa agtgtctgac catgcgctgg acgtggtgat gtttgctgaa gcgaacgaag
     7081 cggaactgga cagcattctg gcgcgccgtc tggctggcga acccctgagc gagcacgaag
     7141 tggcacagtt caaaactgcg gtactggtgt tccttggtgc cgaatatgca cgtcgcggct
     7201 gggtacagca gtaccacatt ggcgcgctgc gtaataacaa cctgcgtcag ttcaaactgc
     7261 tggggccgga tgtaggcttt gactccatca acgaccgtcc gatggcggaa gagttgtcta
     7321 agctgctgag caagcagaac gaagaaaacc tgctgccgaa aaccattctc tactgcctga
     7381 acccgcgcga taacgaagtg ctgggcacca tgatcggtaa cttccagggc gaaggtatgc
     7441 cgggcaaaat gcagttcggt tccggctggt ggtttaacga tcagaaagac ggtatggaac
     7501 gtcagatgac ccaactggcg cagctcggtc tgctgagccg ctttgtcggt atgctgactg
     7561 acagccgtag cttcctgtca tacacccgtc acgaatactt ccgccgcatt ctgtgccaga
     7621 tgatcggtcg ctgggtggaa gcgggcgaag caccggcgga catcaacctg ctgggcgaga
     7681 tggtgaaaaa tatttgcttt aacaatgcgc gtgactactt cgccattgaa ctgaactaag
     7741 gtctggggtt gatatgcaat acatcaagat ccatgcgctg gataacgtcg cggtcgcttt
     7801 agcagatttg gctgaaggca cagaagtcag tgtcgataac cagactgtta cgctgcgcca
     7861 ggatgttgct cgtggacata aatttgcgtt aacggatatc gcaaaagggg ccaacgtcat
     7921 caaatatggc ctgccgattg gttatgcatt ggcggatatt gcggcaggtg ttcacgttca
     7981 cgcccacaat acgcgcacga atctgagcga tctggatcag tatcgctatc aacctgattt
     8041 tcaggatctg cctgcgcaag cggcagatcg tgaagtgcag atctatcgtc gcgctaacgg
     8101 cgatgtcggg gtgcgtaatg agctgtggat cctgccaacc gtgggctgtg tcaacggcat
     8161 cgcgcggcaa atccagaacc gtttcctgaa agagaccaac aacgccgaag gtaccgacgg
     8221 cgtgttcctc ttcagccaca cctacggctg ctcacaactg ggcgacgatc acatcaacac
     8281 ccgcaccatg ctgcaaaaca tggtgcgcca cccgaacgcg ggcgcagtgc tggtgattgg
     8341 tctgggctgt gaaaacaacc aggttgccgc attccgtgaa acgttgggcg atatcgatcc
     8401 tgaacgcgtt catttcatga tctgccaaca gcaggatgat gagatcgaag ccgggatcga
     8461 gcatttgcat cagctgtata acgtgatgcg caacgataaa cgcgagccag gcaaactcag
     8521 cgaactgaag tttggtctgg agtgcggtgg ttctgacggt ctttctggta ttactgctaa
     8581 cccgatgctg gggcgtttct ctgactacgt gattgctaac ggcggtacta ccgtactgac
     8641 cgaagtgccg gagatgtttg gcgcagagca gttgctgatg gaccattgcc gcgacgaagc
     8701 aacgtttgaa aaactggtca ccatggtcaa cgacttcaaa cagtacttta ttgcccatga
     8761 ccagccgatc tatgaaaacc catcgccggg gaacaaagcg ggcggtatca ccacgctgga
     8821 agataaatcc cttggctgta cccagaaagc gggttccagc gtcgtggttg acgtgctgcg
     8881 ttacggcgag cgtctgaaaa cgccggggct gaacttgtta agtgcgccgg gtaacgatgc
     8941 cgtagcgacc agcgccctgg cgggtgcggg ctgccatatg gtgctgttca gtactggtcg
     9001 tggcacgcca tacggcggct ttgtaccgac ggtgaaaatc gctaccaaca gtgaactggc
     9061 ggcgaagaaa aaacactgga tcgactttga cgcgggtcag ctgatccacg gtaaagcgat
     9121 gccgcagttg ctggaagaat ttatcgacac catcgttgag tttgccaacg gtaaacaaac
     9181 ctgcaacgag cgtaacgact tccgcgaact ggcgatcttc aaaagcggcg taacgctata
     9241 aagtcgaaat gagttgttaa taaaaaacgg cgtttcataa tgtgaagcgc cgtttgcttt
     9301 tccatacctg ataaggattt atcatgaccg cgtattggct ggcccagggc gtgggtgtca
     9361 tcgcctttct gattggtatc acaacatttt tcaatcgtga cgaacgtcgc ttcaaaaagc
     9421 agctttcggt ctatagcgcc gttattggcg tacatttttt tcttctgggc acctatcccg
     9481 ctggtgccag tgccatcctt aatgccattc gtacattgat taccttacgc acgcgcagct
     9541 tatgggtaat ggcgattttt attgtgctga ctggcggaat tggcctcgcg aagttccatc
     9601 atcctgtcga actattgccg gttatcggta cgattgtcag tacctgggcg ctgttccgct
     9661 gtaaagggct caccatgcgc tgcgtgatgt ggttttcaac gtgttgctgg gtgattcaca
     9721 acttctgggc ggggtcgata ggcggcacaa tgattgaggg tagttttctg cttatgaatg
     9781 gcttgaatat cattcgtttc tggcggatgc agaaaagggg aattgatccg tttaaagtag
     9841 agaaaacccc tcccgccgta gacgaaaggg gttaaacaat taattacgca gggcgctatt
     9901 tgccagacga tcgtcttccg cctggcaagc tgccgcagtg aacagcacgt cagttgaaga
     9961 gttcagcgcg gtttcgcaag agtcctgcaa tacgccgatg ataaagccga cggcaaccac
    10021 ctgcatggcg atatcgttcg aaataccgaa catattacag gccagcggga tcagcagcag
    10081 agaacccccc gccacgccgg atgcgccaca ggcacacaga gaagccacca cgctcaacag
    10141 cagcgccgtg ggcagatcga ccgggatacc cagcgtatta accgcagcca gcgtcaacac
    10201 ggtaatggtg attgctgcgc ccgccatatt gatggtggca cccagcggaa tagaaacgga
    10261 ataggtatcg cgatccagat tcagcttttc acacagtgcc atattcaccg gaatgttcgc
    10321 cgcagagctg cgggtgaaga aggcatacac gccgctttcg cgcaggcaca gcagcaccag
    10381 cgggaacggg ttacgacgaa ttttccacca caccagcaat gggttaacca ccagcgccac
    10441 cagtaacata cagccaacca gcacgaccag cagttgcgcg tagccccaca gtgtggagaa
    10501 accggtggtt gccagggtag aagaaaccag accaaaaata ccaatcggtg cgaagcgaat
    10561 gaccagtttc accataaagg tgacggcatt cgacatatcg ttgaccaggt ttttggtggt
    10621 ctcgttaccg tgacgcagtg cgaaaccgag gccaattgcc cacaccagga tcccgatgta
    10681 gttacctttc agcagcgcgt cgatggggtt ggaaaccatg ctcattacca gcccgcgcat
    10741 cacttcgaca atgcctgacg gcggcgaaat atcacccgcg ctactggata aatgcagggt
    10801 agaagggaag gcaaagctga agactactgc ggccagagca gcagagaagg tacccagcag
    10861 atagaggaac aaaatagggc ggatattggt tttctgcccg tgctggtggt tagcaataga
    10921 tgccatcacc agcatcaaca ccaggatggg ggcaacggct ttcagtgcgc cgacgaacaa
    10981 agtacctaac agaccaacag cttccgccgc gggttttgag atccatgcca gaagaatccc
    11041 cagaacaagg ccgaccagga tttgttttac caggctgcca tgagccagac gccggaatag
    11101 ccccggtgaa cgttgcgtag tcatttttcg atcctttcat tgtgttgtcg cacatccctg
    11161 gtgcgttctg tcgacgcatc tttcaggatg taacaaagtg tttgcaaggt cgagtataag
    11221 gaaagttaca gcttcgggaa gcgaaaaatg ctggatttta aagagaggtc atattttgca
    11281 acttaattgt tacatttgtt tgacataaac agagggaagg actggctact aaggctgatt
    11341 agataacata tgggcaaaac tatttggaat atgagaacgg ttgcgctcct ttaatgtgtt
    11401 atgccatata aa
//
LOCUS       DAZGVQ010000156        11401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000156 DAZGVQ010000000
VERSION     DAZGVQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.157"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..431
                     /locus_tag="ACHLAG_004156"
     CDS             <1..431
                     /locus_tag="ACHLAG_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="catalyzes the formation of (1 R,6
                     R)-2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     in menaquinone biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFP0657286.1"
                     /translation="GHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFA
                     SLNDDQRRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSK
                     FRALAAELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            446..1303
                     /gene="menB"
                     /locus_tag="ACHLAG_004157"
     CDS             446..1303
                     /gene="menB"
                     /locus_tag="ACHLAG_004157"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFP0657287.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            1303..2265
                     /gene="menC"
                     /locus_tag="ACHLAG_004158"
     CDS             1303..2265
                     /gene="menC"
                     /locus_tag="ACHLAG_004158"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFP0657288.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            2262..3617
                     /gene="menE"
                     /locus_tag="ACHLAG_004159"
     CDS             2262..3617
                     /gene="menE"
                     /locus_tag="ACHLAG_004159"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFP0657289.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            3727..3993
                     /gene="pmrD"
                     /locus_tag="ACHLAG_004160"
     CDS             3727..3993
                     /gene="pmrD"
                     /locus_tag="ACHLAG_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFP0657290.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(3987..4373)
                     /gene="arnF"
                     /locus_tag="ACHLAG_004161"
     CDS             complement(3987..4373)
                     /gene="arnF"
                     /locus_tag="ACHLAG_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFP0657291.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(4373..4708)
                     /gene="arnE"
                     /locus_tag="ACHLAG_004162"
     CDS             complement(4373..4708)
                     /gene="arnE"
                     /locus_tag="ACHLAG_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFP0657292.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            complement(4705..6357)
                     /gene="arnT"
                     /locus_tag="ACHLAG_004163"
     CDS             complement(4705..6357)
                     /gene="arnT"
                     /locus_tag="ACHLAG_004163"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFP0657293.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            complement(6357..7247)
                     /gene="arnD"
                     /locus_tag="ACHLAG_004164"
     CDS             complement(6357..7247)
                     /gene="arnD"
                     /locus_tag="ACHLAG_004164"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFP0657294.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            complement(7244..9226)
                     /gene="arnA"
                     /locus_tag="ACHLAG_004165"
     CDS             complement(7244..9226)
                     /gene="arnA"
                     /locus_tag="ACHLAG_004165"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFP0657295.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            complement(9226..10194)
                     /gene="arnC"
                     /locus_tag="ACHLAG_004166"
     CDS             complement(9226..10194)
                     /gene="arnC"
                     /locus_tag="ACHLAG_004166"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFP0657296.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            complement(10198..11337)
                     /gene="arnB"
                     /locus_tag="ACHLAG_004167"
     CDS             complement(10198..11337)
                     /gene="arnB"
                     /locus_tag="ACHLAG_004167"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFP0657297.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
BASE COUNT         2776 a         2962 c         3057 g         2606 t
ORIGIN      
        1 gcgggcatcc ggggctgcaa aatgctgaac aacgtgcgga acgtcagcgt tccgatcgcc
       61 aatgggcgca gcgttttcgc acagaaccgt taacggcggt atttgccgac tggtatcaac
      121 agcctgtttt tgcctcgctc aatgacgatc aacgccggga gctggtggcg ctgcgcagca
      181 acaataatgg cgcaaccctt gccgccatgc tggaggcgac ttctctcgcc gtccagcctg
      241 atttacgtgc taaccttagc gcccgcacat ttgcgtttta ttatttatgt ggtgaacgtg
      301 acagcaaatt ccgcgccctg gcggcggaac tggctgccga ctgccatgtc attcctcgcg
      361 ccggacataa cgcgcatcgg gaaaatcccg ctggcgttat cgcaagtctg gcgcagatct
      421 tgcgtttctg actaaaggac acaatatgat ttatcctgat gaagcaatgc tttacgcacc
      481 ggttgaatgg cacgactgct ccgaaggttt cgaggacatt cgttatgaaa aatccaccga
      541 cggtatcgca aaaatcacca ttaatcgtcc gcaggtgcgc aatgccttcc gtcctctgac
      601 ggtaaaagag atgatccagg cactggcaga tgcgcgttat gacgacaata tcggcgtgat
      661 cattctgact ggcgcaggcg ataaagcgtt ctgctccggt ggtgaccaga aagtgcgtgg
      721 tgattacggc ggctataaag atgattccgg cgtacatcac ttgaacgtgc tggacttcca
      781 gcgtcagatc cgtacctgtc cgaaaccggt tgtcgcgatg gtggctggct actccatcgg
      841 cggtggtcac gttctgcaca tgatgtgcga cctgactatc gcagcagata atgccatctt
      901 cggtcagact ggcccgaaag tcggttcctt cgacggcggc tggggcgctt cctacatggc
      961 tcgtatcgtc gggcagaaaa aagcgcgaga aatctggttc ctgtgccgtc agtacgacgc
     1021 aaaacaggcg ctggatatgg gccttgtcaa caccgtggta ccgctggcgg atctggaaaa
     1081 agaaaccgtc cgttggtgcc gcgaaatgct gcaaaacagc ccgatggcgc tgcgctgcct
     1141 gaaagctgcg ctgaacgccg actgtgacgg gcaggcgggg ctgcaggagc tggcgggcaa
     1201 cgccaccatg ctgttctaca tgacggaaga aggtcaggaa ggtcgcaacg ctttcaacca
     1261 gaaacgtcag cctgatttca gcaaatttaa acggaatccg taatgcgtag cgcgcaggta
     1321 taccgctggc agatccccat ggacgcgggg gtggttctgc gcgacaggcg gttaaaaacc
     1381 cgcgacgggc tgtacgtttg cctgcgtgag ggcgagcgcg aagggtgggg ggagatctcc
     1441 ccactgccgg gcttcagcca ggaaacctgg gaagaggcgc aaagcgtgct gcttgcctgg
     1501 gtaaataatt ggctggcagg cgattgcgag ctaccgcaga tgccttccgt tgcctttggc
     1561 gtaagctgtg cattggcgga gctggcagat acgctcccgc aggcggccaa ttaccgtgcg
     1621 gcaccactgt gtaatggcga tcctgacgat ctgatcctca aacttgctga tatgccaggc
     1681 gagaaagtgg cgaaggtcaa agtgggattg tacgaagccg tgcgcgacgg catggtggtt
     1741 aatctgttgc tggaggcaat tccggatctg catttgcgtc ttgacgcaaa tcgcgcctgg
     1801 acaccgctga aaggccagca gtttgccaaa tatgttaacc cggattatcg ccaccgcatc
     1861 gcgtttctcg aagagccgtg caaaacccgc gatgattcgc gagcgtttgc ccgtgaaacc
     1921 ggcattgcca ttgcctggga tgaaagcctg cgcgagccgg attttgcctt tgtggctgaa
     1981 gagggcgtgc gcgcggtagt tatcaaaccc acgctcacgg gcagtctgga gaaagtacgc
     2041 gagcaggtac aggcggcgca cgcgctgggg ctgacggcgg tgatcagttc ttccattgaa
     2101 tcgagcttag gcttaacgca actggcgcgg attgccgcct ggttaacgcc ggacaccatt
     2161 ccagggctgg acacgctgga tctgatgcag gcgcagcagg tacgtcgctg gccgggtagc
     2221 acgctgcctg tcgtggaagt tgatgcactg gagcggttgt tatgatcttc tctgactggc
     2281 cgtggcgtca ctggcggcaa gtgcggggag aagccatcgc cttacgtctt aatgacgagc
     2341 aacttaactg gcgcgagctt tgtgctcgcg tcgatgaatt agcctcaggc tttgcggcgc
     2401 agggcgttgt tgagggcagc ggcgtgatgt tgcgggcgtg gaatacgccg caaacgctgc
     2461 tcgcctggct ggcgttactg caatgcgggg cgcgggtgtt gcccgtgaac cctcagctgc
     2521 cgcaaccgtt gcttgaagaa ttgctgccca atctgacgct gcaatttgcg ctggtgccgg
     2581 atggggaaaa cacgtttccg gcgttaacgt cgctgcacat tcagcgggtt gaaggcgcac
     2641 atgccgctac gtggcagccg acgcgtctgt gctcaatgac gttgacctcg ggttctaccg
     2701 ggttgccgaa agccgctgtc catacttatc aggcccatct tgccagtgcc gaaggggtgt
     2761 tatcgttgat tccgtttggc gatcacgatg actggttgct ctctttaccg ctgtttcacg
     2821 tctccggtca gggaattatg tggcgctggt tatacgctgg tgcgcggatg acggtgcgtg
     2881 ataaacagcc attggagcaa atgctggcag gctgtactca cgcttcactg gtgccaacac
     2941 aactctggcg tttgctggtt aaccgcagtt ccgtttccct gaaagcggtg ttacttggcg
     3001 gcgcggctat cccggtcgag ttgacggaac aggcgcgcga gcaggggatt cgttgctttt
     3061 gcggctatgg tctgaccgag tttgcctcca cggtgtgtgc gaaagaagcc gacggcctgg
     3121 cagacgttgg ttcgccgctg ccgggtcggg aagtgaaaat cgttaataat gaagtgtggc
     3181 tgcgggctgc cagtatggca gaaggttact ggcgtaacgg gcaactggtt tcactggtta
     3241 atgacgaagg ctggtacgct acgcgcgatc gcggtgagat gcataatggc aagctgacca
     3301 ttgtcggacg tttagacaat ctgttcttca gtggcggtga gggtattcag ccggaagaag
     3361 tcgagcgtgt tattgctgca catcctgcgg ttttgcaggt gtttatcgtt cccgttgccg
     3421 acaaggagtt tggtcatcgg ccggtagcgg tggtggagta tgaccagcaa accgttgatc
     3481 ttggcgaatg ggtgaaagat aagctggctc gttttcaaca accggtgcgc tggctgactt
     3541 taccgccgga gctgaaaaac ggcggtatta aaatttcacg tcaggcgcta aaagagtggg
     3601 tgcaacgtca gcaataatta accctccgtt tcacttctca tttctgccat tccattgccg
     3661 ttgataaaga gtttatctgt taatctgtaa attgatgtga aaactcttag caaacaggat
     3721 aatgcaatgg aatggctggt caaaaaatcg tgctgcaata aacaagataa cagacatgta
     3781 cttatgctct gcgatgctgg cggcgcgata aaaatgatcg ccgaagtgaa gagtgacttc
     3841 gccgtgaaag tgggggattt actctcgcct ttgcagaatg cgctttattg tattaatcgt
     3901 gaaaagctgc atacggtgaa agtcctctcg gcaagcagtt atagccccga tgagtgggaa
     3961 cggcagtgta cagcggcagg gaaaactcag taacgttgtt ttgtcgtggg caggaaaatc
     4021 agcatcaacc cgctcataat acaggctact cccagtagtg ctttcagcga aaaagtccct
     4081 tcccagccgg gaagtaccat tgaagcaatc cataccagca cataactcat actgagcaag
     4141 gcataggctt tgcttagcgc aagtttatgt agcgttttat accaacaaaa aacggaaagc
     4201 agataaccca gcagcccgag taacagtatc cgggcatcca ggccaaaagc taacagagcc
     4261 gcgataaaat cccataagtg cgtcatcggc ggcagatgac ttgccgcaaa acccaggctt
     4321 aattgcgcaa ctgaggcaat aatgacgctg aataatcccc atatcaggcc cattacaccg
     4381 tactcccgag gatcacaatg ccaccaataa tgaacgccac cccacaccag tgacgcggcg
     4441 ataccggttc gtgccacagt tttactgcag ccagcgtcac ccagacaaaa ttcagactta
     4501 acatcgggta ggcaatgcct accggtacgt tctgcaagac cagcagccag agcaccatgg
     4561 caagaccaag acaagccagc gccagtccca gccacagcac gatatgtttg cgccgtttgt
     4621 ttatcgccac aaagcaggtt gcctgtttct gacacaactg cccggcaacg ctaagcaagc
     4681 tggcaaagac taatgttagc cagatcattt gggacgatac tgaatcagca ccagacgctc
     4741 ctgacgatcg atggcatctg cgggcggaat ggcgagactg ttgatatctt catcgcggtc
     4801 aaccgaaagc acgagagtaa taatcccctc ctgacgatgt tgattaagcc agtttgcgaa
     4861 ctcatcaccg ctgacaaatc tccctttcgc atccggataa ttaaggccgt atttcaactc
     4921 acctgtctgg cgatacatga tgatgtcatc gcgttgcagg ctccatgcca gacctgcggc
     4981 aacaccgacg ctatcagtaa gaatatagcg gcttggctga agtgattctt gtgtcatctc
     5041 gacaaaaaat tgcggatgtt ttccttccat aactctgtca ggaattgaaa atcctaccag
     5101 caacgccagc cccagcgggc aaagcgcggc aaaaggccag gtcttttcga cgtttgttaa
     5161 ggtgtaccag ccgaaaaatg cccatagcga aaaaatcgac caggcacaaa agactttata
     5221 gctctcgaag gtttgccaca ccggcgtgtt cattggtccc cacggggaga ccacaaatgt
     5281 ggcaataatg ccagtgacgc caaaagcgat gttaatccag ccattaatcc gcagcgccag
     5341 aggattattt tttgctgcca gcaaagcgta atgcgccatc agcattgcca gagatgcaaa
     5401 gcaggaaaga atataggtgg gcaatttacc tttagcgacg gagaaaaaca gcagcggcat
     5461 tatcgtccag ctcaacaaat agacggttgc ggaatgcttg cggtttttcc agcctgtgta
     5521 cagtgcaccg gggagtaatc ccagccacgg caggctaccg gcaatgatga ccggcacgta
     5581 gtaccagaac ggagctctat gttgggcatc atccagtgca aagcgttgaa tatgctcaac
     5641 ccagaaaaaa tagtgccaga agtcaggctc ccgctgcgct atcgccagtc cccaagggag
     5701 aaccgtcagt acgcaactga taaccgccag ccagccgtaa ataaagagat ctttccagcg
     5761 tttttgcgtc gctacccatg gcagcacgct taataccggc acggcaaggg cgagaaaacc
     5821 tttggtcatc acccccatgc cgcaggtgat tcccagcagt aaaaatcctg cgcttttgcc
     5881 tttccacgtc tgtgcctgca ttgccagcca gaagctgcac attcccgcca ccagccagaa
     5941 tgcaataaac ggatcgagca cggcataagt gccgatggca tagacaataa acaatgagag
     6001 ataaattact gtggcgagta gagccagacg tttatcgcgc cataagcgca gagtaaacca
     6061 ggtcaccagc gcggcagtta acagggtcgc aaagataacg cctgcccgca caccaaagtt
     6121 attcgcgcca aatagccatt gcccaatgct gttaatccag tatccggcaa tgggtttttc
     6181 gaaataacgt agccctaaca gatggggcac aatccagtcg ccggatgcca gcatttctcg
     6241 actgatttcc gcataacgcg tttcatcggg ttgccagagc agacgcgtgc tgatcggtaa
     6301 caggtaatag caggcaataa acgcgaagag gccgataagg taacgtaccg atttcatcag
     6361 cgactacccg caatttgttg gcaacccagc cagccttcac gtccggcaat atttccgcga
     6421 acaacttgtc cgagcggcaa cgtctctgat aacagttcgc ttaaagggca aaatgtcacg
     6481 ccttcctgag ctgcgcgttt gaggagatcc acaaaattat gctgataagc gcagccttca
     6541 acttctgcat gaatggtata aaccggcgtg cctttatctc gcaggatgcg gttgagtaac
     6601 caaccgttaa aatcttctgc tttcacatcc cgaccaatca cttcatccca ggtgggtaag
     6661 gtcaccggaa tttgcgcagt gccagggttt cctgattcga gcaatggacg gaacggcatg
     6721 gccccacgac aatcgctgtt gtagcgcaaa tggaacgctt cttttgcttc gatcaccttc
     6781 tggtcggcac gccagcccgc agcggcagaa caggttaccg gttgaccgat aatctcttcc
     6841 agagtgcgaa gaccgcgtgc aatatcgtcg atcattgttt gccgatccca gttaccgcta
     6901 cgggcttgcc aggcatggtg atcccaggcg tgcaggccga cttcgtgatg tttagccgct
     6961 tcacgaatga tatcggcatt ggcatgacca atctctttac ctggccaggc cgtacctgcc
     7021 agtaaaatat cccagccata aagcgatgcc gcatttgagc gcagcatctt ccacaaaaac
     7081 tgtggcttca ccagtcgcca gagatggcgg cccatattgt ccgggccgac gctgaaaaaa
     7141 atgctggcct gaatattatg cttactcaag atttccagca gacgcggcac gccttcacgg
     7201 gtgccacgaa aggtatcgac atcaatgcgt aagcctactt tggtcatgat ggtttatccg
     7261 taagatcaac ggtgcgcagg aagaaatcca gcgtttcgtc gatggtttcc tgcatatcaa
     7321 ttttcggctc ccagtccagg cagcggtggg cattgcggat gctcggttta cgatgctcta
     7381 cgtcctgata tcctttgccg tagtagctgc tactttcgac aacgcgaaag cccgcaaacg
     7441 gtgggaaatg atggcgcagc ggatgttttt cgaagctcgc cagcagcatc tcgcccagtt
     7501 cctcaatgct cgcttcgttc tcaggattgc caatgttgat aatttcaccg tcgcagcgat
     7561 ttcccgcatt ttcgataatg cggtataacg cctcgatacc atcgcgaata tcagtaaagc
     7621 agcgtttttg ttttccgcca tcaatcagct taatcggtga accttctacc agattgagaa
     7681 tgagttgcgt aatagcgcgg gagctgccga ttcgtgccgc attaaggtta tccagtcgcg
     7741 gtcccatcca gttaaacggg cggaagaggg tgaactgtaa accctctttt tcgccatagg
     7801 cccagatcac ccgatcaagt aattgttttg ataccgaata aatccagcgt ggtttattca
     7861 ccgggccgac gattaaatta gaatggtcct catcgaagta tttatcgcta cacatcccat
     7921 aaacttctga agttgacggg aagatgattc gcttacggta cttcacgcag tagcggataa
     7981 tgcgcagatt ctcttcaaaa tcgagttcaa atacgcgcag cgggttgcgg gtatattcaa
     8041 tcggcgtggc tatcgccacc agcggcaaga cgacatcaca ttttttgacg tgatactcaa
     8101 tccattcgga atgaatactg atatcgcctt caacaaagtg aaaatgcgga tgattcagaa
     8161 aacggcttat cgcatcactg ccaatatcca gaccgtaaac ttcataatga tcttcgcgca
     8221 gcaggcgttc tgtcagatgg ttgccaataa agccattcac cccgaggatg agtacccggg
     8281 tacggcgtcg ggcggtgcag gcaggctggc tattcaagcg tgaaccttgc accaggccca
     8341 gcgtctgcgc taattgcgag ccctgcatag taatgccgtc gcccgcctgt ccggtgacga
     8401 tttccagcgc gccatcgcca caggcaatca gcagtggcgc aacagaaatc acgctccccg
     8461 gctgtgcttt gctggcatga ggatgaacac gcgacgacca gacggtgaat ttctgattgc
     8521 caacatagct gaaggcaccc ggccacggat cggcaacggc acgtaccatg ttgtgcagta
     8581 cggatgccgg tttatgccat tcaaggaaac tgtcatccgg cgttctgcga ccaaaacagg
     8641 tggcttcgtt ttcgcgctgg gcgatttcca gaatattacc gtgtttaatg gcgggtaatg
     8701 tctgttccag tagctggcgc gcggcatggc acaatttatg atgcagcgta atagcgatat
     8761 cgtctggcgc aatggcaatg cgcagttgcg ccacaatggc cccggcatcg gcacgtttca
     8821 ccattcggtg caatgtaacg ccagtttccg tttcaccgtt gaccagcacc cagttcagcg
     8881 gcgcgcgacc acgatatttt ggtaacagcg aaccatgcag attaaatgca cctgcgggag
     8941 cgagctgcaa aatttcgtcg caaataagat ggcgataata aaaagagaaa atcacatctg
     9001 gcgacagttg ggcaatgcgt tccacccaca gcggatgatt aacgtcatcc ggcgcataaa
     9061 ccggaatgcc tctttccgct gccagacgag ccaccgaacc ataaaaggct ttttcaccgg
     9121 gattatcggt atgggtaaaa atggcgctaa tttcgtaacc ggcagccagc agagcttcaa
     9181 taccgaggca tcccatatcg tggtaggcaa aaacgacggt tttcattatt cattttcctt
     9241 gctggatgga cggataactt gctgaacaaa atagcggggg cgggcgcgga catcggtgta
     9301 gatcctgccg atatattcac cgagtaatcc catgccgata aactgagcgc caataaaagt
     9361 aaacagcacg gcaaatagca taaagacgcc ttctgccgcc cattgtggtc cgaaggtcag
     9421 gcgtaaaatc accagcagca ccgcaatgct aaaacctcca atcgcaataa tgctgccgag
     9481 cagactcagc atacgtagcg gcgttgtggt aaggcaggtc accaggtcgt acatcaaatt
     9541 aatcaggcgc ataaaactgt atttggattc accaaactca cgctcggaat gatgtactgg
     9601 aatttcaatg gcacggcggg cgaagatatt cgccagaatc gggataaagg tgctacgttc
     9661 atggcagtgc aacatcgcat cgacaatatg acggcgatag gcgcgcagca tacaaccgta
     9721 gtcacccatc gctttgccgg tggtgcgctg aataagccgg ttaatcatct tcgaagctgt
     9781 tttacgaaac cagctgtcct ggcggttctg gcgtacagtc cctaccacgt cgtaaccttc
     9841 atcggctttt gccaccaggc gggggatttc ttctggcgga ttctggagat cggcatcaag
     9901 ggtaataatt aagtcgccgg taacgtgact gaatcccgcc ataatcgctg aatgttgccc
     9961 gtaattgcgg ttaagcaaaa tagacacaat atggctgttc tccgcttgtg aggcttcgac
    10021 cagcatatgc gcggaattat cgctactgcc gtcatcaatc agcaggatct catactcttt
    10081 ccccaacgat tcacaggctg tggtggtgcg cctgattaat tccggtaagc tttcctgctc
    10141 gttataaacg ggaataacca ccgagacttt cttaacaggg tggatttcaa acatggctta
    10201 ttgtcctgcg agttgctgaa gggctgtgat gacacggtcg gcatcggcgg tagtcatatc
    10261 cgggaacagc ggcaaagaac agatgcgttc gctattccat tcggtattcg gtaacgacag
    10321 cgtgggaaaa cgctcgcgat aatatttttg tgtgtgagcg gcgcggaaat gtaacccggt
    10381 accaatgcct ctttctttta acgcttccat caacgcatcg cgactgatac cacaacgttg
    10441 ttcatcgaca cgaataataa acagatgcca ggcgtgaacg tgcggccagg cgggaaggct
    10501 taatggctga aaggggagag ctgccagtgc ttgctgatat tgctgggcaa tttcgcgccg
    10561 acgggtgttg aggtgctcta attttgctaa ctgtgtcagg gcaatcgcgg cgttaatatc
    10621 ggtcagattg tacttatagc ccggtgttaa gacttcagcc tgcggtgcac ggccccaggt
    10681 ttgtctgtca taggcatcga cacccagacc gtgaaatttc agcatccgta gctggcgggc
    10741 aaggttttca ttatcagtta caatcaggcc accttccgca caggtaatat ttttaatggc
    10801 atgaaatgaa aaaatagcgg taccttttgc gccaatatgt cgccctttgt aatacgtacc
    10861 gacggcatgg gcagcatctt cgataactgc gatgccgtaa cgttcgccaa tggcgcgaat
    10921 ggcgtcaata tctgctggtg caccggcata atgcaccgga atgatggctt tagtgcgtgg
    10981 cgttatggct gactcgatcg cttcaggcgt gaccatcagc gtatcgcggt cgacatccac
    11041 cattaccggc gttgcaccca acaaggaaat catattgagg gttgaaaccc aggtcaggga
    11101 aggcgtaatc acttcatcgc ccttgccaat ttccaacgcc attagcgtga tatgcattcc
    11161 ggcggtggct gaactgaccg cgatggcatg ctgatttccc gtcaactggc aaaaggcttg
    11221 ctcaagcgcc tgattcttcg gaccggttgt gatccaaccg gattcgagaa cctctttaac
    11281 tgcagcgagt tcctccacgc ccattgctgg tcgcgaaaaa ggcaaaaatt ctgacattgc
    11341 ttttccttcc gccattgaat acctgtccac ttatatttgc tataaagagt gttgtgtata
    11401 t
//
LOCUS       DAZGVQ010000157        11274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000157 DAZGVQ010000000
VERSION     DAZGVQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.158"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..902
                     /locus_tag="ACHLAG_004168"
     CDS             <1..902
                     /locus_tag="ACHLAG_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFP0657298.1"
                     /translation="CISPAKFHECFINWMRDCHSSNDKDVIAIDGKTLRHSYDKSRRR
                     GAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIV
                     EKIQKQGGDYLFAVKGNQGRLNKAFEEEFPLKELNNPEHDSYAISEKSHGREEIRLHI
                     VCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFAT
                     AIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLR
                     RKMRKAAMDRNYLASVLAGSGLS"
     gene            1171..3408
                     /gene="nrfB"
                     /locus_tag="ACHLAG_004169"
     CDS             1171..3408
                     /gene="nrfB"
                     /locus_tag="ACHLAG_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFP0657299.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            3395..6367
                     /gene="nfrA"
                     /locus_tag="ACHLAG_004170"
     CDS             3395..6367
                     /gene="nfrA"
                     /locus_tag="ACHLAG_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFP0657300.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDASAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            6368..7258
                     /locus_tag="ACHLAG_004171"
     CDS             6368..7258
                     /locus_tag="ACHLAG_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFP0657301.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7441..8202
                     /gene="envY"
                     /locus_tag="ACHLAG_004172"
     CDS             7441..8202
                     /gene="envY"
                     /locus_tag="ACHLAG_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFP0657302.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            8714..9667
                     /gene="ompT"
                     /locus_tag="ACHLAG_004173"
     CDS             8714..9667
                     /gene="ompT"
                     /locus_tag="ACHLAG_004173"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFP0657303.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYIF"
     gene            9854..>11274
                     /locus_tag="ACHLAG_004174"
     CDS             9854..>11274
                     /locus_tag="ACHLAG_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0657304.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHL"
BASE COUNT         3065 a         2767 c         2730 g         2712 t
ORIGIN      
        1 cctgtatcag tcctgcaaaa tttcatgagt gctttattaa ctggatgcgt gactgccatt
       61 catcaaatga taaagacgtc attgcaattg atggaaaaac gctccggcac tcttatgaca
      121 agagtcgccg caggggagcg attcatgtca ttagtgcgtt ctcaacaatg cacagtctgg
      181 tcatcggaca gatcaagacg gatgagaaat ctaatgagat tacagctatc ccagaacttc
      241 ttaacatgct ggatattaaa ggaaaaatca tcacaactga tgcgatgggt tgccagaaag
      301 atattgtaga gaagatacaa aaacagggag gtgattattt attcgcggta aaaggaaacc
      361 aggggcggct aaataaagcc tttgaggaag aatttccgct gaaagaatta aataatccag
      421 agcatgacag ttacgcaatc agtgaaaaga gtcacggcag agaagaaatc cgtcttcata
      481 ttgtttgcga tgtccctgat gaacttattg atttcacgtt tgaatggaaa ggtctgaaga
      541 aattatgcgt ggcagtctcc tttcggtcaa taatagcaga acaaaagaaa gagcccgaaa
      601 tgacggtcag atattatatc agttctgctg atttaaccgc agagaagttc gccacagcaa
      661 tccgaaacca ctggcacgtg gagaataagc tgcactggcg tctggacgtg gtaatgaatg
      721 aagacgactg caaaataaga agaggaaacg ccgcagaatt attttcaggg atacggcaca
      781 tcgctattaa tattttaacg aatgataagg tattcaaggc agggttaaga cgtaagatgc
      841 gaaaagcagc catggacaga aactatctcg cgtcagtcct tgcggggagc gggctttcgt
      901 aatcttgccc tgcctgctca cctcctccag gcacttccac tacattatga cctgctcggt
      961 gccaccgctc cttttcacta tgacgttaac ctcccctcct tgacaccatg cttttgtcta
     1021 cattctacgc aaacggcaac ttttataacc agtgtaaaaa taacgtgccg caatagtcat
     1081 acctattaat ggtaaaaagc tgtcacaatt cataaaaaac cttaatatac gccaccctaa
     1141 acataaccag cgttaatgta aggtttttgt gtggactggc ttcttgatgt ttttgcaacc
     1201 tggctctacg gcttaaaagt aatcgcgata acgttagcgg tcatcatgtt catcagcggg
     1261 ctggacgatt tttttattga tgtcgtctac tgggtacgcc gcattaaacg caagttgagt
     1321 gtttatcgcc gctacccgcg aatgagttac cgcgaactgt ataaaccaga tgaaaaaccg
     1381 ttagcgatta tggttccggc gtggaatgaa acgggcgtca tcggcaatat ggccgagctg
     1441 gcggcgacca cgctcgacta cgaaaactat catatctttg ttggcaccta ccccaacgac
     1501 cccgatactc aacgtgatgt tgacgaagtg tgcgctcgct ttccgaacgt gcataaggta
     1561 gtctgcgcgc gtcctggtcc caccagcaaa gccgactgtc tgaacaacgt gctggacgcc
     1621 atcacccagt ttgaacgtag cgccaatttc gcttttgctg gttttattct gcatgacgcc
     1681 gaagatgtga tttcaccgat ggaattgcgt ctgttcaact atctggtcga gcgtaaagat
     1741 ctgattcaga tcccggtgta tccgttcgaa cgcgaatgga cgcacttcac cagcatgact
     1801 tacattgatg agttttcaga gctgcatggc aaagatgttc cggtgcgtga agccctcgcc
     1861 ggacaagtgc ccagcgcagg cgtcggcacc tgtttcagcc gccgcgccgt gaccgctctg
     1921 ttagctgacg gtgacggtat tgctttcgac gtgcagagtc tgactgaaga ttacgacatt
     1981 ggtttccgcc tgaaagagaa aggtatgacg gaaatttttg tccgttttcc ggtggtggac
     2041 gaagccaaag aacgcgagca gcgcaaattt ttacagcacg cacggacgtc aaacatgatc
     2101 tgcgtgcgcg aatatttccc cgataccttt tcgactgcag ttcgacaaaa atctcgctgg
     2161 atcatcggca ttgttttcca gggctttaaa acccacaaat ggacctccag cctgacgctg
     2221 aactactttc tctggcgcga ccgcaaaggg gcaatcagta actttgtcag cttcctcgcg
     2281 atgctggtga tgctccagct tttgctgttg ctggcgtatg aaagtttgtg gcccgatgcc
     2341 tggcatttcc tttctatttt tagcggcagc gcatggttaa tgaccctgct gtggctaaac
     2401 tttggcttga tggttaaccg catcgtgcag cgggtgattt tcgtcactgg ctactacggt
     2461 ctgacgcagg ggctgttatc tgtcctgcgt cttttctggg gcaacctgat taacttcatg
     2521 gccaactggc gcgcgctaaa acaggtactt caacacggcg atccacgtcg cgtcgcgtgg
     2581 gataaaacaa cgcatgactt ccccagcgtg actggcgata cccgctcgtt gcgcccgtta
     2641 ggccaaattc tgctggaaaa tcaggtcatc actgaagaac aactcgatac agcactgcgt
     2701 aatcgcgtcg aaggtctacg cctgggcggt tcaatgctga tgcaggggct gattagcgcc
     2761 gagcagctgg cacaggcgct ggcagagcaa aacggcgtgg cgtgggaatc catcgatgcc
     2821 tggcagatcc cgtcctcgct gattgccgaa atgccggcct ccgtagcgct gcattatgca
     2881 gtactgccgc tgcgtctgga aaatgatgag ttaattgtcg gcagtgaaga tggtattgac
     2941 ccggtttcgc tggcggccct gacgcgtaaa gtcggacgca aagtgcgtta cgtcattgtt
     3001 ctgcggggac aaattgtcac ggggttacgt cactggtatg cacgccgacg cggtcacgat
     3061 ccgcgggcaa tgttgtacaa tgcggttcag catcagtggc tcacggaaca gcagaccggt
     3121 gaaatctggc ggcaatatgt gccgcatcag ttcctgttcg ccgaaatact gaccacgctc
     3181 ggtcatatta atcgttcagc aattaacgtg ttgttattgc gccatgaacg cagttctctg
     3241 ccgctcggca agtttttggt caccgaaggc gttatcagcc aggaaacgtt ggatcgcgtc
     3301 ctgacaattc aacgcgaatt acaagtttcg atgcaatcac tattactcaa agcaggttta
     3361 aacacagaac aggttgcgca actggagtcc gaaaatgaag gagaataacc ttaatcgcgt
     3421 catcggatgg tctggtttac tgctgacgtc tttattgagt accagcgcac tcgcagacaa
     3481 tatcggcacc agcgcagaag agctggggct gagcgattat cgccattttg ttatttatcc
     3541 ccgtctcgat aaggcgctga aggcacagaa aaataacgac gaagcaaccg ccatccgcga
     3601 atttgaatat atacaccagc aggtgccgga taatattccg ctgactttat accttgcgga
     3661 agcctatcgc cattttggtc atgatgaccg ggcgcggctg ttgcttgagg atcaactgaa
     3721 acgtcaccca ggagatgccc gacttgagcg cagtctggcg gctattccgg ttgaagtgaa
     3781 aagcgttacg actgttgaag aactgcttgc ccagcaaaaa gcgtgcgacg ctgcgccgac
     3841 cctgcgttgt cgcagtgaag tcgggcagaa tgccctgcgg ctggcacaat tacctgtcgc
     3901 cagagcgcaa ctgaacgatg cgacgtttgc tgcatcgcca gaaggaaaaa cgctgcgaac
     3961 cgatctgctg caacgggcaa tctacctgaa acaatggtcc caggcagata cgctatacaa
     4021 tgaagcacgc cagcagaaca cattaagcgc ggcagaacgc cgtcagtggt ttgacgtgct
     4081 tcttgccggg cagctggacg atcggatcct ggcactgcaa tcacagggga tcttcaccga
     4141 tcctcagtca tatattactt acgcgaccgc gctggcttat cgtggcgaaa aagcacgcct
     4201 ccagcattat ctcaatgaaa ataagccgct gtttaccacg gacgcacaag agaaaagttg
     4261 gctctatctg ttatctaaat acagcgccaa ccccgttcag gcattggcga attatacggt
     4321 gcagtttgcc gataaccgcc agtatgttgt tggcgcgacg ctaccggtgc tgttaaaaga
     4381 aggtcagtac gacgcagcgc aaaaactgct cgccaccctc cccgccaatg aaatgcttga
     4441 ggagcgttat gctgtcagcg tggcgacccg taacaaggct gaatctctgc gtctggcacg
     4501 attgctgtat cagcaagaac cggcaaatct tacccgcctg gatcaactga cctggcaact
     4561 gatgcagaac gagcagtcac gcgaagctgc cgatttattg ctgcaacgct atcctttcca
     4621 gggcgatgcg cgtgtcagcc agactttaat ggcgcgactg gcgtctctgc tggaaagtca
     4681 tccttacctg gcaacgccgg cgaaggtggc gattttatcg aaacccttac cgctggcgga
     4741 gcaacgtcag tggcaaagtc agttgccggg tattgcagat aattgcccgg caatagttcg
     4801 cttgctgggc gatatgtcgc cttcctacga tgcttccgcc tggaaccgtc tggcaaagtg
     4861 ttatcgggac acgctacccg gtgtggcgct gtatgcatgg cttcaggccg aacaacgaca
     4921 accgaacgcc tggcaacatc gtgcggtagc ctatcaggcg tatcaggttg aggactacgc
     4981 caccgcactg gcggcctggc agaaaatcag tcttcacgac atgagcaatg aggatctgct
     5041 tgctgctgcc aataccgccc aggcggcagg aaatggtgca gctcgcgatc gctggctaca
     5101 acaggcagaa caacgtggac tgggaaacaa tgccctctac tggtggctgc atgcgcaacg
     5161 ttacattcct ggtcagccgg aactcgcact gagcgatctc acgcgctcaa tcaatattgc
     5221 cccttctgcc aacgcttacg ttgcgcgggc gacaatttat cgccaacgtc ataatgtccc
     5281 ggcggcggtg agtgatttgc gcgccgcgct ggaactggaa ccgaataata gcaacatcca
     5341 ggcagcgctt ggttacgcct tgtgggatag cggtgatatc gcacagtcgc gggaaatgct
     5401 cgaacaggcg cataaagggc ttccggacga tccggcactg atccgacaac tggcctacgt
     5461 gaaccagcgt ctggatgaca tgcctgcgac gcagcactac gcccggctgg tgattgatga
     5521 cattgataat caggcgctga taaccccact gaccccagag caaaatcagc aacgcttcaa
     5581 tttccgccgt ttgcatgagg aggtcggtcg ccgctggacg ttcagtttcg attcttctat
     5641 cggcttgcgt tccggggcaa tgagcaccgc taacaataat gtcggcggcg cagcgccagg
     5701 gaaaagctat cgtagctacg ggcaactgga agccgagtac cgcatcggac gcaatatgct
     5761 gctggaaggc gacctgctct cagtttatag ccgcgtcttt gccgataccg gagaaaacgg
     5821 ggtgatgatg ccggtgaaaa atccgatgtc cggcaccggt ctgcgctgga agccgctgcg
     5881 cgatcagatc tttttcctcg ccgtcgaaca gcagttgccg ctgaacggcc aaaatggcgc
     5941 atccgatacc atgctgcgcg ccagcgcctc attctttaat ggcggcaaat acagcgacga
     6001 atggcacccg aacggttcag gctggtttgc ccaaaacctg tacctcgatg cggcgcaata
     6061 tatccgccag gatattcagg cgtggacggc agattatcgc gtcagctggc atcagaaggt
     6121 agctaacgga cagactattg agccttacgc tcacgttcag gacaacggct atcgtgataa
     6181 aggcactcag ggcgcgcagc ttggcggagt cggggtccgc tggaatatct ggaccggcga
     6241 gacgcactac gacgcctggc cgcacaaagt cagtctcggc gtcgagtatc aacatacctt
     6301 taaggcgatt aatcaacgta acggagagcg caacaacgcg tttctcacca ttggagtgca
     6361 ctggtaaatg cgtaagttca ttttcgtatt gctgacactg cttttggtca gccctttttc
     6421 ctttgcgatg aaaggtatta tctggcaacc acaaaaccga gatagtcagg ttaccgatac
     6481 ccagtggcag gggctgatga gtcagttacg tttgcaaggc ttcgataccc tggttttgca
     6541 atggacccgt tacggcgatg cattcaccca gccagaacag cgcgcgttat tgtttaagcg
     6601 tgccgcagct gcgcaacagg ctggcctgaa gctgattgtc gggctgaacg ccgatccgga
     6661 attttttatg caccagaaac agtcgtccgc agcgctggaa agctatctta atcgcctgct
     6721 ggctgccgat ctccagcaag ccagattatg gagcgccgcg cctggcgtaa cgccggatgg
     6781 ctggtacatc agcgcggaaa ttgacgacct gaactggcgc agcgaagctg cccgtcagcc
     6841 tttgctaaca tggttaaaca acgcgcagcg gctgattagc gatgtttcag caaaaccggt
     6901 ttatatcagt agttttttcg ccggaaacat gtcgcccgat ggctatcgcc aactgctgga
     6961 acaagttaaa gcaaccggcg ttaatgtctg ggtacaggat ggcagcggcg tggataaact
     7021 taccgctgaa cagcgtgaac gttatctaca ggccagcgcc gattgccaaa gtcccgcccc
     7081 tgccagcggc attgtttatg aactgtttgt cgccggcaaa ggcaaaacct ttacagcgaa
     7141 accgaaaccg gatgcagaga tcgcctcgct gttagcgaaa cgttcctctt gcggtaaaga
     7201 cactctctat ttctctctgc gctatttgcc cgtcgcgcac ggcattctcg agtattaaat
     7261 ctcctccagg taagtcgggt acgacctggc ttacctcttt cgctctcgtc agaattccat
     7321 caagattaat tgctaaaaag cgtgctaaag aaagaagatc gttatcggcg aacgggccga
     7381 tcactcatgc aggaagcata gatcctttga gcggagtttc tgatatctga ggagtgcgaa
     7441 atgcaattga gcagcagtga accttgcgtg gtgatcctga ccgaaaaaga ggtagaggta
     7501 agcgtcaata accatgctac gtttaccctt ccgaaaaact acctggccgc cttcgcgtgc
     7561 aacaataacg tcattgaact ctcaacgtta aatcacgtat taatccccca catcaaccgt
     7621 aacatcatca acgattatct gttgttttta aataagaact taacctgtgt aaagccctgg
     7681 tcgcggctgg caaccccggt tatcgcttgt catagccgta caccggaagt gttccggcta
     7741 gccgccaacc acagcaagca gcaacccagc aaaccctgcg aagcggagtt gacgcgcgca
     7801 ttacttttta ccgtattgtc taactttctt gagcaatcgc ggtttatagc cctactgatg
     7861 tatatcttac gcagcagcgt ccgcgacagc gtctgccgca ttattcaaag cgatattcag
     7921 cattactgga atctgcgaat tgtcgccagt tcgctatgtt taagccccag cctactcaaa
     7981 aagaaattaa aaaacgaaaa taccagctat agccagattg tcacagagtg tcgtatgcgt
     8041 tacgccgtac agatgttatt gatggataac aaaaatatca ctcaggtggc gcaattatgt
     8101 ggctatagca gcacgtcgta ctttatctct gtttttaagg cgttttacgg cctgacaccg
     8161 ttgaattatc tcgccaaaca gcgacaaaaa gtgatgtggt gaagggcaaa gcggaaacgg
     8221 ataagacggg cataaatgaa gaagaaatgg cgcgccctgc aggattcgaa cctgcggccc
     8281 acgacttaga agttcctaga acgacatttt aagtcaacaa cttaccacgc catctctgcg
     8341 ctcacacgtc ccactacctc aaaacatgta aagccttgca agccattgtg aggccttatg
     8401 tgtctcagtt ttgtccctct tttttgtact aaaaaacata gcaattgagg ataaacctca
     8461 tgctattttc gcttatatgc ctctaaaggc atggcgctta aatagataaa aacaccacaa
     8521 aagcataaaa aaccacacag taaaaccgca atataaaaca ataacagata attaaaccaa
     8581 aaacagatag cgcattgtga taatcattca atactaaaca aaatataaac agtggagcaa
     8641 tatgtaattg actcattaag ttagatataa aaatacatat tcaatcatta aaacgattga
     8701 atggagaact tttatgcggg cgaaacttct gggaatagtc ctgacaaccc ctattgcgat
     8761 cagctctttt gcttctaccg agactatatc gtttactcct gacaacataa atgcggacat
     8821 tagtcttgga actctaagcg gaaaaacaaa agagcgcgtt tatctagctg aagaaggagg
     8881 ccgaaaagtc agtcaactcg actggaaatt caataacgct gcaattatta aaggtgcaat
     8941 taattgggat ttgatgcccc agatatctat tggggctgcg ggctggacta ctctcggcag
     9001 ccgaggtggc aatatggtcg atcaggactg gatggattcc agtaaccccg gaacctggac
     9061 agatgaaagt aggcaccctg atacacaact caattatgcc aacgaatttg atctgaatat
     9121 caaaggctgg ctcctcaacg aacccaatta ccgcctggga ctcatggccg gatatcagga
     9181 aagccgttat agctttacag ccagaggtgg ttcctatatc tacagttctg aggagggatt
     9241 cagagatgat atcggctcct tcccgaatgg agaaagagca atcggctaca aacaacgttt
     9301 taaaatgccc tacattggct tgactggaag ttatcgttat gaagattttg agctaggtgg
     9361 cacatttaaa tacagcggct gggtggaagc atctgataac gatgagcact atgacccagg
     9421 aaaaagaatc acttatcgca gtaaagtcaa agaccaaaat tactattctg ttgcagtcaa
     9481 tgcaggttat tacgtaacac ctaacgcaaa agtttatgtt gaaggcgcat ggaatcgggt
     9541 tacgaataaa aaaggtaata cttcacttta tgatcacaat gataacactt cagactacag
     9601 caaaaatggt gcaggcatag aaaactataa cttcatcact actgctggtc ttaagtacat
     9661 cttttaacga agttaaccag attctctccc cggtggtatt gtataacccc ggggaatttg
     9721 tttacagtta caagtagaca atatccacta attttgtaag tatttaacct atgtcataac
     9781 gtgtaatctc ttgccgcaac acaagacctc aatatattca aattttatct ttattccgat
     9841 attcagagac acaatgcgaa aaataattac tcatttcaaa gttgttttaa cgttacttct
     9901 accagtaacc gtatctgccc agcagataca gtggcaatcc tgtatggcca gtcaattcaa
     9961 ccactggttt ggtgaggaaa aaccgtctcc tgacttacta tgtggttatt tgtctgttcc
    10021 attaaaatat acagacacag gcggagatgc ttcttatgaa aaaaaatcac aagtcaaact
    10081 agcgttgaca aaattgccgg caaaaagcaa gcataaagga agtatcctga taataagtgg
    10141 tggtcccggg ttaccaggca taaatcctta tattaacttt gactggccag tcacaaatct
    10201 tcgtgagtca tgggatatta ttggatttga tcctcgaggc gtcggacagt ccactccgac
    10261 aataaactgc cggcaatcag atacagagac tcaggaaaac ataaccgaaa agcaacaagt
    10321 attaaataaa attaatgcct gtatccataa taccggagcc gaagtcattc gccatatcgg
    10381 ctctaacgag gctgtatacg atattgatcg tattaggcaa gccttggggg ataaacaact
    10441 gacagccgtg gcgtattcgt atggaactca aattgcagcc ttatatgcag aacgttttcc
    10501 ctacaacgta agatctatcg ttcttgatgg agtcgtcgat atcgatgacc tggaggacaa
    10561 cttcacatgg caactcaaac aggcacagag ttatcaggaa acgtttgatc gctttgcatc
    10621 ctggtgtgcg cgtacaaaaa gttgcccgct ttcttcagac agagataagg caataactca
    10681 gttccatgag ctattatcaa aattacatca caaaccttta ttagacagta agggagaaaa
    10741 tatatcttca gatgaactca tatcattaac aacagacctt ctgctatggc gttcatcatg
    10801 gccaaccctt gcaactgcca tacgccagtt ctctcagggg attgtcagta atgaaattga
    10861 aactgcgctc agtgctccga tagcctcaga agagtcaagc gatgcttcgg gggtaatcct
    10921 ctgtgtagat cagggggatg agcaattaac accagaagag cgaaaatccc gaaaagacgc
    10981 tcttgcgaat gccttcccgg ctattaactt tgacaatgga cgttccgatt cacctgattt
    11041 ttgtgaatta tggccaatac atagcgacct gaacaaaact cgcctgaaaa atactgttct
    11101 gccctctggt ttactgtttg tagcacacaa atacgaccca acaacgccct ggattaatgc
    11161 ccgtaagatg gcagagaaat tttccagccc gttactaaca ataaatggtg atgggcatac
    11221 attagctctc accggagtta atttatgtgt agataaagca gttgtacatc acct
//
LOCUS       DAZGVQ010000158        11262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000158 DAZGVQ010000000
VERSION     DAZGVQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.159"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            104..646
                     /gene="ecpR"
                     /locus_tag="ACHLAG_004175"
                     /gene_synonym="matA"
     CDS             104..646
                     /gene="ecpR"
                     /locus_tag="ACHLAG_004175"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFP0657305.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            721..1308
                     /gene="ecpA"
                     /locus_tag="ACHLAG_004176"
                     /gene_synonym="matB"
     CDS             721..1308
                     /gene="ecpA"
                     /locus_tag="ACHLAG_004176"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFP0657306.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFTII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1366..2034
                     /gene="ecpB"
                     /locus_tag="ACHLAG_004177"
                     /gene_synonym="yagY"
     CDS             1366..2034
                     /gene="ecpB"
                     /locus_tag="ACHLAG_004177"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFP0657307.1"
                     /translation="MKKHLLPLALLFSGIPPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2060..4585
                     /gene="ecpC"
                     /locus_tag="ACHLAG_004178"
                     /gene_synonym="yagX"
     CDS             2060..4585
                     /gene="ecpC"
                     /locus_tag="ACHLAG_004178"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFP0657308.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4575..6218
                     /gene="ecpD"
                     /locus_tag="ACHLAG_004179"
     CDS             4575..6218
                     /gene="ecpD"
                     /locus_tag="ACHLAG_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFP0657309.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQATGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6187..6897
                     /gene="ecpE"
                     /locus_tag="ACHLAG_004180"
     CDS             6187..6897
                     /gene="ecpE"
                     /locus_tag="ACHLAG_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFP0657310.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7210..7539
                     /gene="ykgJ"
                     /locus_tag="ACHLAG_004181"
     CDS             7210..7539
                     /gene="ykgJ"
                     /locus_tag="ACHLAG_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP0657311.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7787..8401)
                     /gene="yagU"
                     /locus_tag="ACHLAG_004182"
     CDS             complement(7787..8401)
                     /gene="yagU"
                     /locus_tag="ACHLAG_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFP0657312.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            8819..9508
                     /gene="paoA"
                     /locus_tag="ACHLAG_004183"
     CDS             8819..9508
                     /gene="paoA"
                     /locus_tag="ACHLAG_004183"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFP0657313.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVFYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAALKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9505..10461
                     /gene="paoB"
                     /locus_tag="ACHLAG_004184"
     CDS             9505..10461
                     /gene="paoB"
                     /locus_tag="ACHLAG_004184"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFP0657314.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            10458..>11262
                     /gene="paoC"
                     /locus_tag="ACHLAG_004185"
     CDS             10458..>11262
                     /gene="paoC"
                     /locus_tag="ACHLAG_004185"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFP0657315.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKV"
BASE COUNT         2893 a         2966 c         2923 g         2480 t
ORIGIN      
        1 tccggtttac taaggaaact gaatgcacct gtaaaaatta caggtttgga aagtagtgac
       61 atggcaaagt gattacagca gggactatga ggttaaaaac catatggaat gtcaaaaccg
      121 ttctgataaa tacatctggt ctccccatga cgcctacttc tataaaggac tatctgaact
      181 gattgtggat atcgacagat taatttatct atcgttggag aaaattagaa aagatttcgt
      241 gtttatcaat ctcaatacgg attctttatc tgaatttata aaccgtgata atgaatggtt
      301 atccgcggta aaggggaaac aggtcgtatt gattgcggcc agaaagtcag aagccttagc
      361 aaattattgg tattacaaca gcaatattag gggcgtggta tacgcaggac tgagtcgtga
      421 tattagaaaa gaactggcct atgtgattaa tggcaggttc ctgagaaaag atattaagaa
      481 agataaaatc actgaccggg aaatggaaat tatccgcatg acggcccagg gaatgcaacc
      541 taaatcgatt gccagaattg aaaattgtag tgtgaagaca gtgtataccc atcggcgtaa
      601 tgctgaggcc aagctgtact caaaaatata taagttggtt cagtaaactc caggcaagtt
      661 agttttaaaa aatgactcac tgggacatca cgtcctcaat tcaactcggg aagaaatgca
      721 atgaaaaaaa aggttctggc aatagctctg gtaacggtgt ttaccggcat gggtgtggcg
      781 caggctgctg acgtaacagc tcaggctgta gcgacctggt cggcaacagc caaaaaagac
      841 accaccagta agctggttgt gacgccactc ggtagcctgg cgttccagta tgccgaaggc
      901 attaaaggtt ttaactcaca gaaaggtcta tttgacgtgg ctatcgaggg tgactcaacg
      961 gctaccgcct ttaaactgac ctcacgtctt atcaccaaca cattaaccca gttggatacc
     1021 tcaggttcca cactgaatgt gggcgtggat tataacggcg cggcagtcga aaaaactggc
     1081 gataccgtga tgatcgatac cgccaacggc gtactgggcg gcaaccttag cccgctggct
     1141 aacggttaca atgccagcaa tcgtaccacc gcacaggatg gtttcacctt caccatcatc
     1201 agcggcacca ccaatggtac caccgcagta accgattaca gcactctacc ggaaggcatc
     1261 tggagcggcg acgttagcgt acagttcgac gcgacctgga ccagttaatc tctctgatgt
     1321 accagcaggg gtagcccccc tgctttattc ctggacggaa ttgttatgaa aaagcacctt
     1381 ctgcctctcg ctctgctgtt ttccggaata cctccggccc aggcgctgga tgtcggcgat
     1441 atatcatcgt ttatgaacag tgacagcagc acgctgagca aaacgatcaa aaacagtacc
     1501 gacagtggtc gccttatcaa tatccgtctc gaacggctct cttcaccgct tgacgacggg
     1561 caggttatct caatggacaa gccggatgag ttgctactca ctcccgccag cttgctgcta
     1621 cccgcccaag ccagcgaagt gatccgcttc ttctataagg gacccgcaga tgaaaaagag
     1681 cgctactacc gcattgtctg gtttgatcag gccctcagtg atgcgcagcg cgataatgcc
     1741 aaccgcagcg ctgtggccac tgcttccgcc cgcatcggca ccattctggt cgtcgcccct
     1801 cgccaggcga actaccactt tcagtacgcc aacggctccc tgacaaatac aggaaatgcg
     1861 acgctgcgga tcctcgccta cggcccttgc ctgaaagccg ccaacggtaa ggagtgtaaa
     1921 gagaattact acctgatgcc gggcaagtcg cgtcgtttta cccgtgtgga cactgcggat
     1981 aacaaaggac gggttgcact ttggcagggt gataagttca ttcccgtgaa atagatagct
     2041 gtgcagatgg ataacgacaa tgcctttacg acggttctcc ccaggactga aagcccagtt
     2101 tgccttcggc atggtctttt tgttcgttca acccgatgcc agcgctgctg acataagtgc
     2161 gcagcaaata ggtggggtga ttattccgca ggccttcagt caggcgcttc aggacggcat
     2221 gagcgtcccg ctctatattc atctcgccgg tagccagggt cgccaggacg atcagcgaat
     2281 cggcagcgct tttatctggc tggatgatgg acagctacgc atccggaaaa tacagctgga
     2341 agagagtgaa gataacgcca gtgtcagcga acaaactcga cagcagctga tggctctggc
     2401 caacgccccg ttcaatgagg cccttaccat ccccctgact gacaacgcgc agctggatct
     2461 cagcttgcgc caactgctgc tgcagctggt ggtcaagcgc gaagcgctgg gcaccgtact
     2521 acgctcacgt agcgaagaca tcgggcagtc cagtgttaac accctcagca gtaatctgag
     2581 ctataacttg ggcgtctata acaaccagtt gcgtaacggt gggagcaaca catccagcta
     2641 tctgtcgctg aataacgtta ctgcgctgcg cgaacatcat gtggtgctcg acggctcgct
     2701 gtacgggatc ggtagcggtc aacaggacag tgaattatat aaagcgatgt atgaacgcga
     2761 ttttgccggt caccgatttg ccggtggaat gctcgacacc tggaacttgc agtccttagg
     2821 gccgatgacc gccatttcag cagggaagat ttacggcctt tcctggggaa accaggccag
     2881 ctccaccatc ttcgacagca gccagtcagc cacgccagtg atcgcctttt taccggcggc
     2941 gggcgaagta catctcaccc gtgatgggcg gctactaagc gttcagaact tcactatggg
     3001 caatcatgaa gtggataccc ggggtctacc gtacgggatt tacgatgtgg aagttgaggt
     3061 gatcgttaac ggtcgcgtga tcagcaaacg cccccagcgg gtcaataagc tgtttagccg
     3121 ggggcgcggc gtcggtgcac cactggcgtg gcaggtatgg ggcggtagct ttcatatgga
     3181 tcgctggtcg gaaaacggga aaaagacgcg accagctaaa gagagttggc tagcaggtgc
     3241 ctcgacctcc ggctcattga gtacgcttag ctgggcggca acgggatatg gatacgataa
     3301 tcaggcggtg ggtgaaaccc gtctgacgct gccgcttggg ggggcgatca acgttaacct
     3361 gcaaaatatg ctggccagtg acagctcatg gagcagcatc ggcagcatca gcgccactct
     3421 accgggaggc tttagttcgc tgtgggttaa ccaggaaaaa acccgcattg gcaatcaatt
     3481 gcgacgtagc gatgccgaca accgtgcaat cggcggcaca ctcaacctga actcactgtg
     3541 gtcgaagctg ggcacattca gcatcagcta caatgatgac cgccgttaca acagccatta
     3601 ttacacggca gattactatc aaaatgtcta cagcggtacc tttggttcgc ttggcctgcg
     3661 ggccggtatt cagcgctata acaacggcga cagcaacgcc aatacaggga aatatatcgc
     3721 tctcgatctc tcgctaccac tgggcaactg gtttagcgca gggatgaccc atcaaaacgg
     3781 ctacaccatg gcaaacctgt cagcacgcaa acagtttgat gaaggaacca ttcgcactgt
     3841 tggtgccaat ctgtcacgtg ccatctccgg cgataccggt gatgacaaaa ccctcagcgg
     3901 tggggcgtat gcacagttcg acgctcgtta cgccagcgga acgctgaacg tcaatagcgc
     3961 ggcggacggc tacatcaata ccaacttgac cgccaatggc agcgtcggct ggcagggtaa
     4021 aaacatcgct gccagcgggc ggactgatgg caacgctggg gtgatattca acaccgggct
     4081 ggaggacgac ggtcagatca gcgccaaaat caacgggcgg attttcccgc ttaacggcaa
     4141 gcgtaactat ctcccgctct ctccctatgg aagatatgag gtggagttac agaacagcaa
     4201 aaactcactc gacagctacg atatcgtcag cggccgcaaa agccatctga ctctctatcc
     4261 aggcaatgtc gctgtcattg agccagaggt gaagcagatg gttaccgtct ccggtcgtat
     4321 ccgtgcggaa gacggcacac tgctggctaa cgcacggatt aacaaccata tcggccgaac
     4381 ccgaaccgat gaaaacggcg agtttgtcat ggacgtggat aagaaatatc ccactatcga
     4441 ttttcgctac agtggcaata aaacctgcga agtggcactg gaactcaacc aggcgcgcgg
     4501 tgccgtctgg gtcggtgatg tggtctgcag cggcctctca tcgtgggcgg cggtgacgca
     4561 gacaggagaa gagaatgaga gttaacctac taataacgat gataattttc gcgctaatct
     4621 ggccagtaac tgagctcaga gcggcagtga gcaaaacaac ctgggcggat gcaccggcac
     4681 gcgagtttgt gtttgtcgaa aacaactcag acgacaactt tttcgtcact cctggcgggg
     4741 cgctggatcc gcgcctgacc ggtgccaacc gctggaccgg tttaaaatac actggttcag
     4801 gaaccatcta tcagcaaagc ctcggctaca ttgataacgg ttacaacacc ggcctttata
     4861 ccaactggaa gtttgatatg tggctggaaa attcaccagt ttcatctcct ttaactggct
     4921 tgcgctgcat caactggtac gctgggtgta atatgaccac cagtcttatc ctgccgcaaa
     4981 ccaccgacgc cagtggattt tatggcgcga cggtgaccag cggcggcgcg aagtggatgc
     5041 acggcatgtt gtcagacgcg ttttaccagt atctgcaaca aatgcccgtc ggcagcagct
     5101 ttacaatgac catcaatgcc tgccagacct ctgtgaacta tgacgccagc agcggcgcac
     5161 gctgtaagga tcaggccacc ggcaactggt atgttcgcaa cgtcacccat acgaaagcag
     5221 caaatctgcg gttaataaat acccactcgc tggcggaggt atttatcaac agcgacggag
     5281 taccgactct gggcgaaggg aacgccgact gccggacaca gaccatcggc agcctttcag
     5341 gattaagttg taagatggtc aactataccc tgcaaacaaa cggactcagc aacacctcaa
     5401 tccatatatt cccggcgatc gccaactcgt cgttagcctc ggccgtcggg gcgtacgata
     5461 tgcagttcag tctgaatggc agttcatgga aaccggtgag caataccgcc tattactaca
     5521 ccttcaacga gatgaagagc gcagactcga tctatgtttt cttctcgagc aacttcttta
     5581 agcagatggt gaacctcggg atcagcgata tcaacaccaa agatctattc aactttcgct
     5641 ttcagaacac cacatcaccg gagtctggct ggtatgaatt ttctacctcc aacacgctga
     5701 ttatcaaacc ccgtgatttc agcatcagta ttatctccga tgaatatact cagacaccgt
     5761 cgcgggaggg atatgttggc agcggcgagt cggcactcga tttcggctat atcgtaacca
     5821 ccagcggtaa aacagctgcc gacgaagtgc tgatcaaggt gaccggaccc gcgcaggtga
     5881 ttggcgggcg ctcctattgt gtcttcagct ccgatgacgg taaggcgaaa gtaccgttcc
     5941 cggcgacact ttcctttatt acccgcaacg gagctacaaa aacctacgat gccgggtgcg
     6001 atgatagctg gcgggatatg accgatgcgc tgtggttgac cacaccgtgg actgatatct
     6061 ctggcgaagt ggggcagatg gataagacca cagtcaaatt ttcgattcca atggataacg
     6121 ccatttcgct gcgtacggta gatgataacg gctggtttgg cgaagtcagc gcttcaggag
     6181 agattcatgt tcaggcgacg tggcgtaaca ttaactaagg ccctgctgac agcggtctgt
     6241 atgctggcgg cacctttgac acaggcgatt tcggtcggca atctgacatt ttcgctgccg
     6301 tccgagactg actttgtcag caaacgtgta gtgaataaca acaaaagcgc acggatatac
     6361 cgtattgcca tcagtgctat tgatagcccg ggcagcagtg aattgcgcac ccgaccggtg
     6421 gatggtgaac tgcttttcgc cccccgccaa ctggcgttgc aggctggtga gagcgagtat
     6481 tttaaatttt actatcatgg tccacgggat aaccgcgagc gctactaccg ggtctcattt
     6541 cgcgaagtcc ccactcgtaa ccagacaaga cgtagcccaa ccggcggcga ggtcagcacg
     6601 gagccggtgg tggtgatgga taccattctg gtagtacgac cacgtcaggt tcagtttaaa
     6661 tggtccttcg acaaggtgac aggaacggta agtaacactg gcaacacatg gtttaagcta
     6721 ctgattaagc caggatgtga ttcgaccgaa gaggaaggcg atgcctggta tctacgtccg
     6781 ggagacgtgg ttcatcagcc tgagttacgt cagccgggga atcattatct ggtctataac
     6841 gacaaattca ttaagattag cgattcctgt ccggctaagc ccccttcggc ggactaagac
     6901 ttatccatcg gtcgagggaa gaattccacc tcagagctcc aaaaatcgtt taaatgatgt
     6961 ggaagcgatc gtcaatgtca cctgtaacga tgggctttct catatgcaca ccatagtgtt
     7021 caggagacac ggaaaggtat caggcagcct gctaatgagt ggtttaatca cgaattagta
     7081 cgtaaaatcg gtaacggctg gaaatcattc aatactcaca ctatcgaaag ttccccagcc
     7141 aaccgcggta cgttcttaca tacgatgtac cgctgttctc tttacgattt ttagctgtac
     7201 tggtgaatta tgagcaatct gaatccatgc atgacgtgtg gtgcctgttg tgcatttttc
     7261 cgcgtctctt tttactgggc cgaagccgac gatgctggcg gtactattcc cgccaggctc
     7321 actgaacaaa tatccccttt tcaccgatgc atgagcggta ccaatcagaa aaacccccga
     7381 tgtattgccc ttgcaggaac cccgggcaaa aatgcctgct gcacgatata taaaaatcga
     7441 tcgtccacat gcagagaatt cgccatgtct ggtgagaacg gagtcgtcaa tgaggcttgc
     7501 aatcgtgcaa gggctaaata cgggctgaca ccgttataaa catacaataa ttaattgcac
     7561 tgccccgcca gcgataatag cggggcttcg tttttcaggg gtaacaaaac ccgatacttc
     7621 ttctatttgc cagcaacaat gcctctcttc tgtagcgttc ttcggatcta catcatcctg
     7681 aagtagcgcg atatactcac tgactatctg catcagaata taaaaagcaa tgtttttaac
     7741 ctataaaaat ggtgctgtat ttgcgccatt tttatcattc aatgcattat ctgtttgagc
     7801 ctaaagggat ctcagggtct ggctcatgag taattctgtt tcgtaaatct ctgcgaataa
     7861 tttcaataga ccagaaccag actaaatgtc caaaaatttc agaaacattc tcataccacg
     7921 ggagatcaaa cagaggtggc gtcagtccca tgagagggaa tgaaatcata tgaacaaaaa
     7981 gttgggctaa agcacctgcc agtaagccct gccagagttt aatttttgga aatacttcag
     8041 cgaccacaca ataaccgaca gcaaacacta tcgaaaagat aatgtgcgta acaccaaccc
     8101 agttaaagac atgcccggca aaggtataaa cagccgcatt gggatctgtc agccccaacc
     8161 agtctcgaag aaaaatatac ggtggattga gaaaattacg cgagcaatca atttggcctg
     8221 cagccctgat taatgattcc gggccacacg ctgcattaaa catatccacc gggctacgtg
     8281 gcggcaatgg aacttcagcc ccccacttca cgaatgcgga aacaacgcca gcaatcagcc
     8341 caatgaatgc agcaagacca taacgtctgc ggttcggtgg agtttgttca aatatattca
     8401 tatctaccct gcttgtacca ttatgttata cacctcttca ggagtattca taaaacaagg
     8461 cgaatgtaaa gaactgtatt gttttgtata acaagatggt ttcctaatcg ccaatgaata
     8521 taagctccat catttctccc tatttttata ttagaagtga cagagatttg cagggtgatg
     8581 cagagctgaa aatcacaggt ttccttattg gtttttgcat cgtacaacta aagcaataaa
     8641 ccagctccca tcatcatcaa catcccggcg aacatgaatt tattagccgg gctcatagct
     8701 gcattcgcgt tttaagaata atcctcctgc tgtcgccgac tatgcttaac atttaaaaaa
     8761 gcatcagcac tctcgcaacg cactcttatt ttccccttta gaataccgga ggcctggtat
     8821 gagcaaccaa ggcgaatacc ccgaagataa tcgggttggg aagcacgagc cgcacgattt
     8881 aagtttgacc cgtcgcgatc tgattaaagt gagcgccgca acagcggcga ccgccgtgtt
     8941 ttatcctcat tctacgctgg cggcaagcgt tccggcagct acacccgcgc cagagataat
     9001 gcccctgaca ctgaaggtga acggcaaaac cgagcagctt gaggtggata cccgaaccac
     9061 gctactggac gctttgcgtg aaaatctgca tttgatcggt accaagaaag gttgcgatca
     9121 cggacagtgc ggagcctgta ccgtgctggt caatggtcgc aggcttaatg cctgcctgac
     9181 gcttgcagtc atgcatcagg gggccgagat caccaccatt gaaggcctgg gttcgccaga
     9241 taatcttcac cccatgcagg cggcctttat caagcatgat ggcttccagt gcggctactg
     9301 cacctccggg caaatttgct catcagtagc ggcgctaaaa gagattcagg acggcattcc
     9361 cagtcacgtc acggtcgatt tggtttccgc tccagaaaca actgccgatg agatccgtga
     9421 acgtatgagc ggcaacatct gtcgctgtgg tgcatacgct aacatccttg ccgccattga
     9481 agatgctgcg ggggagataa aatcatgaag gcgtttacct atgaacgagt gaacacccca
     9541 gccgaggcgg cacttagcgc tcagcgcgta cccggcgcaa aatttatcgc gggcgggacc
     9601 aatctgctgg acctgatgaa gctggaaatt gaaacgccca cccaccttat cgatgtgaac
     9661 ggcctcgggc tcgataagat tgaagtgacc gacgcgggtg ggctgcgcat cggcgcactg
     9721 gtacggaaca ccgacctggc ggctcacgag cgcgtgcgtc gtgattacgc ggtactctcc
     9781 cgcgccctgc tcgctggcgc atctggtcag ttacgtaatc aggcaaccac cgcaggtaat
     9841 ctgctccagc gcacgcgctg cccctatttt tacgacacca atcagccctg caataagcgc
     9901 ctgcccggga gcggctgcgc ggcgcttgaa ggctttagcc gtcagcacgc ggtggtaggc
     9961 gtaagtgaag cctgcatagc cacccatccg agcgatatgg cggtcgcaat gcggttgctg
    10021 gatgcggtgg tggaaaccat cacgccggag ggaaagactc gcagtatcac actggctgat
    10081 ttttatcacc caccggggaa aacgccgcac attgaaaccg ccctgcttcc cggtgagctt
    10141 atcgttgcgg tgacgttacc tccaccgctc ggcggaaaac atatctaccg taaggtgcgc
    10201 gatcgcgcct cctacgcctt tgccctggta tcggtcgcgg cgattattca gcctgacggc
    10261 agcgggcgcg tcgcgctggg cggagtagca cataagccct ggcgcattga ggctgcggat
    10321 gctcagctat cccagggggc gcaggccgta tatgacgcgc tgttcgccag cgcccatccc
    10381 accgctgaaa atacctttaa actcctgttg gcgaagcgaa cgcttgcctc cgtactggct
    10441 gaagcgaggg cacaggcatg aaatttgata aacccgcagg ggaaaacccg atcgatcagc
    10501 tgaaggttgt cggtcgtccc catgaccgca tcgacggacc gctgaaaact accggcacgg
    10561 cacgctacgc ctacgaatgg catgaagaag cccccaacgc cgcctatggc tatatcgtcg
    10621 gttccgccat tgccaaagga cgcctcaccg cccttgatac ggacgccgcg caaaaagcgc
    10681 cgggcgtact ggctgtcatt accgccagta acgccggggc actcggcaaa ggcgacaaaa
    10741 acaccgccag actgttaggc ggccccacta ttgagcacta tcatcaggcc attgcgctgg
    10801 tagtggccga gaccttcgaa caggcgcgag cggcggcctc gctggtgcag gcacactatc
    10861 gccgtaataa aggagcttac tccctggcgg acgaaaaaca ggccgtcaat cagccgccgg
    10921 aagacacgcc cgacaaaaac gtcggtgact ttgacggggc tttcacctcc gctgcggtga
    10981 agattgatgc tacctacacg accccggacc agagccatat ggcgatggag ccgcatgcct
    11041 cgatggccgt ctgggatgga aataagctta ccctctggac ctcaaatcag atgattgact
    11101 ggtgccgcac cgatctggca aaaacgctga aagttcccgt ggagaatgtg cgtattatct
    11161 ccccgtatat cggcggaggg tttggcggca agctgttcct gagaagcgat gcgctgctgg
    11221 cggccctcgc cgcccgagcg gtgaaacgtc cggttaaagt ga
//
LOCUS       DAZGVQ010000159        11225 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000159 DAZGVQ010000000
VERSION     DAZGVQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11225
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.160"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..128
                     /locus_tag="ACHLAG_004186"
     CDS             <1..128
                     /locus_tag="ACHLAG_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP0657316.1"
                     /translation="YLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA"
     gene            107..211
                     /gene="speFL"
                     /locus_tag="ACHLAG_004187"
     CDS             107..211
                     /gene="speFL"
                     /locus_tag="ACHLAG_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFP0657317.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            522..2720
                     /gene="speF"
                     /locus_tag="ACHLAG_004188"
     CDS             522..2720
                     /gene="speF"
                     /locus_tag="ACHLAG_004188"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFP0657318.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2717..4036
                     /gene="potE"
                     /locus_tag="ACHLAG_004189"
     CDS             2717..4036
                     /gene="potE"
                     /locus_tag="ACHLAG_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFP0657319.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4092..5732)
                     /gene="pgm"
                     /locus_tag="ACHLAG_004190"
     CDS             complement(4092..5732)
                     /gene="pgm"
                     /locus_tag="ACHLAG_004190"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFP0657320.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5758..6303)
                     /gene="seqA"
                     /locus_tag="ACHLAG_004191"
     CDS             complement(5758..6303)
                     /gene="seqA"
                     /locus_tag="ACHLAG_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFP0657321.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6488..7252
                     /gene="ybfF"
                     /locus_tag="ACHLAG_004192"
     CDS             6488..7252
                     /gene="ybfF"
                     /locus_tag="ACHLAG_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP0657322.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7392..7685
                     /gene="ybfE"
                     /locus_tag="ACHLAG_004193"
     CDS             7392..7685
                     /gene="ybfE"
                     /locus_tag="ACHLAG_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFP0657323.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7825..8355
                     /gene="fldA"
                     /locus_tag="ACHLAG_004194"
     CDS             7825..8355
                     /gene="fldA"
                     /locus_tag="ACHLAG_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFP0657324.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8565..8651
                     /gene="uof"
                     /locus_tag="ACHLAG_004195"
     CDS             8565..8651
                     /gene="uof"
                     /locus_tag="ACHLAG_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFP0657325.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8644..9090
                     /gene="fur"
                     /locus_tag="ACHLAG_004196"
     CDS             8644..9090
                     /gene="fur"
                     /locus_tag="ACHLAG_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFP0657326.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9174..9500)
                     /gene="chiQ"
                     /locus_tag="ACHLAG_004197"
     CDS             complement(9174..9500)
                     /gene="chiQ"
                     /locus_tag="ACHLAG_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFP0657327.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9550..10956)
                     /gene="chiP"
                     /locus_tag="ACHLAG_004198"
     CDS             complement(9550..10956)
                     /gene="chiP"
                     /locus_tag="ACHLAG_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFP0657328.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDSTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPNAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2716 a         2757 c         2803 g         2949 t
ORIGIN      
        1 attatttggg gtatgatcgt tttttgttga tcttcttcac agattatagc catttcatgg
       61 atagaataac tctaccttca actgacacag caagaggtaa aggtaaatgg aaaataataa
      121 ccgcttaatg cctcatataa ggcggacaac ccatatcatg aagtttgccc atcgtaatag
      181 ttttgacttt catttcttta atgcccgcta gtcttctgac taaagggcac cccaacgtac
      241 aggtctccct gactttaagc attacaggtt aatacctgta ttcctcggtg ctcatatact
      301 gctaaccctt ttaaactcta aataattcga gtcgcagcac ttgcaacttg aggtatgacg
      361 agtatagcca gttaccgggc tggtctgggt tattgcatct gcaaaaagca aactactgat
      421 ttatttatca gcggtggagc tttgcttttt ttccggcgtg atcgatttct cctttgagaa
      481 attgaggacc tgctattacc tgaaataaag agatgaacaa aatgtcagaa ttaaaaattg
      541 ccgttagtcg ttcttgcccg gattgttttt ccactcatcg tgcatgcgtg aatatagacg
      601 aaagtaatta tattgacgtt gccgccatta ttttatcagt cagtgatgtt gaacgtggaa
      661 aactcgatga aatagacgct actggctatg acattcctgt ttttattgca acggaaaatg
      721 aagaacgtgt acccgcagaa tatctgccac gtatttctgg tgtcttcgaa cattgcgaat
      781 cacgtaaaga gttttatgga cgtcagttag aaaccgctgc cagccattat gaaacgcaac
      841 tgcgcccgcc cttcttccgc gcactggtcg attatgttaa tcagggcaat agtgcttttg
      901 actgccctgg tcatcagggg ggtgagtttt tccgtcgcca tccggcaggc aatcagttcg
      961 tggaatactt tggcgaaatg ctcttccgct ctgacttgtg taatgctgac gtagcaatgg
     1021 gcgatctgct gattcatgaa ggtgcgccat gtattgcaca acagcatgcg gctaaagtct
     1081 ttaatgccga taaaacctat ttcgttttaa acggtacttc atcttctaat aaagtggtct
     1141 taaacgcatt gctgacgccg ggcgatctgg tgttgtttga ccgcaataac cataaatcta
     1201 accaccacgg tgcgttgcta caggctggcg cgacaccggt ttatctggaa accgcacgta
     1261 acccgtatgg ttttatcggt ggtattgacg ctcactgttt tgaagaaagt tacctgcgtg
     1321 agctgattac agaagttgca ccgcagcggg caaaagaggc tcgtcctttc cgcctcgccg
     1381 ttattcagct aggcacttac gacggtacga tttataacgc ccgtcaggtg gtggataaaa
     1441 ttggtcatct gtgtgactac attctgtttg actccgcatg ggttggctat gagcaattta
     1501 tcccgatgat ggctgactgt tcgccgctgt tgctggatct taatgagaac gatccgggta
     1561 ttctggttac gcaatccgta cataaacagc aggctggttt ttctcaaact tcacaaatcc
     1621 ataaaaaaga cagccacatc aaagggcaac agcgttatgt accgcacaaa cgcatgaaca
     1681 acgcctttat gatgcacgcc tccaccagcc cgttctatcc gctgtttgcc gcactggata
     1741 tcaacgccaa aatgcatgaa ggtgtcagcg gtcgtaatat gtggatggat tgtgtggtaa
     1801 atggcattaa tgcccgcaaa ctgatcctcg ataactgtca gcatattcgt ccgttcgtac
     1861 ctgaactggt ggatggtaaa ccctggcagt cgtatgaaac agcgcaaatt gcggttgatc
     1921 tgcgcttctt ccagtttgta ccaggggaac actggcattc ttttgaaggc tatgcagaga
     1981 atcaatactt tgtcgatcct tgcaaactgt tgctgacaac cccaggtatt gatgcacgta
     2041 acggcgaata tgaagcgttc ggtgtacccg cgacgattct tgctaacttc ctgcgcgaaa
     2101 atggcgtagt gccggaaaaa tgcgatctta actccatcct cttcctgctg actccggcag
     2161 aagatatggc caagcttcag caacttgttg ccctgctggt acgcttcgaa aaactgcttg
     2221 agtccgacgc gccattagca gaagtgctgc cttccatcta caaacagcat gaagagcgct
     2281 acgccggtta taccctgcgt cagttgtgtc aagaaatgca tgatttgtat gcccgtcaca
     2341 acgtgaaaca actgcaaaaa gagatgttcc gtaaggaaca cttcccacgc gtaagcatga
     2401 atccgcaaga agccaactac gcctatttac gcggtgaagt ggagctggtt cgtctgccgg
     2461 atgcagaagg ccgtatcgct gccgaaggtg cgcttcctta tcctccgggt gtgctgtgcg
     2521 ttgttccggg tgaaatctgg ggtggtgctg ttctgcgtta cttcagcgct ctggaagaag
     2581 ggatcaacct gttgccaggt tttgcaccgg agcttcaggg tgtctatatc gaagaacatg
     2641 atggtcgtaa gcaagtttgg tgctatgtca tcaagcctcg tgatgcgcaa agtaccctgt
     2701 tgaaagggga aaaattatga gtcaggctaa atcgaacaaa atgggcgtcg ttcagttaac
     2761 catactgacg atggtcaaca tgatgggctc cggtatcatc atgctgccga caaagcttgc
     2821 cgaagttggg acaatttcaa ttatctcctg gctggtgaca gccgtaggct caatggcact
     2881 ggcatgggca ttcgcaaaat gcggtatgtt cagccgtaaa tcaggtggta tgggcggtta
     2941 tgccgagtat gctttcggta agtccggcaa ttttatggcg aactacacct acggtgtctc
     3001 gctgctgatt gctaacgtcg cgattgctat ttcggcggtt ggttacggca ccgaactgct
     3061 cggcgcgagt ttgtcgccag tgcagattgg tcttgcgacc atcggagtgc tgtggatttg
     3121 taccgtggct aactttggtg gtgcgcgcat taccgggcaa atcagtagca ttactgtgtg
     3181 gggggtcatt attccggtgg taggcctgtg cattatcggc tggttctggt ttagcccgac
     3241 gctttacgtt gattcctgga atccgcatca tgcaccgttc ttcagtgcag taggttcttc
     3301 catcgctatg acgctgtggg cttttctcgg tctggagtct gcgtgtgcga atactgatgt
     3361 agtggaaaac ccggaacgta atgtgccaat cgcggtactc ggcggtacgt taggtgcggc
     3421 ggtgatttat atcgtctcca ccaacgtgat tgcagggatt gtgcctaata tggagctggc
     3481 aaattcaacg gcaccatttg gcctggcctt cgcacagatg ttcacaccag aagtgggtaa
     3541 agtcattatg gcgctgatgg tgatgtcctg ctgcggttcg ctgcttggct ggcagttcac
     3601 cattgcccag gtgtttaaat cttcatctga tgaaggctac ttccctaaaa ttttctcccg
     3661 tgtaaccaaa gtggatgcac cggtgcaggg aatgttgacc attgtgatta tccagagcgg
     3721 gttggcgctg atgacgatta gcccgtcgct aaacagtcag ttcaacgtgc tggttaacct
     3781 ggccgtggtg accaatatca ttccgtatat tctgtcaatg gcggcattag tcattattca
     3841 gaaggtcgct aatgtgcccc cctcaaaagc gaaagttgca aactttgttg cttttgttgg
     3901 cgcgatgtat agcttttatg cgctgtactc atccggggaa gaagccatgc tgtacggttc
     3961 catcgtgacc ttccttggtt ggacactgta tggtctggta tcaccacgct ttgaactgaa
     4021 aaataaacac ggttaaaaaa gcaaagtaaa aaagggcgat cttgcgaccg cccttttttt
     4081 attaaatgtg tttacgcgtt tttcagaact tcgctaacaa tctcaaccgc ttctttctca
     4141 atctgcttgc gatgttcttc accgaggaag ctttcgcagt agatcttata tgcgtcttcc
     4201 gtgcctgacg gacgcgcggc gaaccagccg ttgtcggtca tcactttcag accgccgata
     4261 gaagcaccat tgcccggtgc cgccgtcagg cgcgcggtga tcgggtcgcc tgcaagggtg
     4321 ctggcgctca ccatttccgg agacagctta gacagcgccg ctttttgtgc ggaagtcgcg
     4381 gctgcctgca aacggttgta gctcggcgca ccaaagcgtt ttgccagttc gttgtagtgt
     4441 tcctgcgggt tcttaccggt gacagcggtg atttccgccg ccagcagaca cataatgatg
     4501 ccgtctttgt cggtggacca cggcgtgccg tcgaaacgca ggaaggaagc ccctgcgctc
     4561 tcttcgccgc caaagccgaa gctgccgtcg aacagaccat caacaaacca tttgaaacct
     4621 accgggactt ctaccagctt acggcccaag tcattgacca cacggtcgat catcgcagaa
     4681 gaaaccagcg ttttaccgac agcaacatct ttgccccact gcggacgatg ctggaacagg
     4741 tagttgatcg ccaccgccag gtagtggttc ggattcatca aacctgccgg agtgacgata
     4801 ccgtgacggt cataatccgg gtcgttagca aacgccagat cgaacttatc acgcagtgcc
     4861 agcaggcccg ccatcgcaca ctcggaggag cagtccatac ggatcgcgcc gtctttatca
     4921 aggtgcataa agcggaaggt ttgatcgacc tgatcgttaa cgatggtcag gttgaggttg
     4981 taatactcgc caatacgctt ccagtattcg ataccggaac cgcccagcgg atcaacgccc
     5041 agcgtcaggc ccgctttctg aattgccgcc atatcaacga tatcggccag accttccaca
     5101 aacggctgca ccagatcctg ctcttttaca tgaccggatg ccatcgcttc gtcgagggag
     5161 atacgcttca cgcctttcag gccatcggcc atcagtgcgt tggccctgtc ttccaccact
     5221 ttggtgacgt tggtatcagc cgggccacca tttggcgggt tgtacttgat accaccatct
     5281 tccggcgggt tatgggacgg tgtaatcacg ataccgtctg ccagcgggcc acctttttta
     5341 ttgtgaacca ggatggcatt ggaaatggca ggtgtcggag tgaagccatt gttttcctgc
     5401 acaatgacat caacgccgtt cgctgccagc acttccagta cggaaatgaa cgcaggctcg
     5461 gacagggcgt gagtatcttt acccacatag caagggccag tgatgccgtt tttcgcacgt
     5521 tcttcagcaa ttgcctgagc gatcgccaga atgtgcggct cgttaaagct gtggcgcgct
     5581 gcactgccac ggtgaccgga agtaccgaat ttcaccgcgt gctccgcatt ccctgcttct
     5641 ggtttcagta cataatattg cgccgtcagt tgggcgacgt taatcaaatc actctgttgt
     5701 gcaggttggc ctgcacgatt gtggattgcc attgctttgt cctttgtctg caacgtttta
     5761 gatagttccg caaaccttct caatcaattc cgccgggaat tgcatcgact gcatgatgtg
     5821 ttcgatcatg ctgcatttac ggccggtgtt ggtgttggtg atcacccaat acggcgtgcc
     5881 tggcacatgt ttcggcttgg tctgattacc atttttcagc agcgtttgtt catctgccgc
     5941 aaagtaaacg cgtgtacgac cgtgcaacga ttccgttgct tcggcaaacg cctgggcgtc
     6001 aagagaatat agtgtagaca acagcagcat aaagcgattg accgctcgct tttgctctgc
     6061 gtattcatcc gaaagcagaa gttcacgcat tgcgcgaacc ttgtctttaa tcgttttgac
     6121 cggcttcgct tcgacgatag caggtgacgc aacgcgaacc tctttcgtca ccggagcagc
     6181 aggctgtgat gcggcggaaa atttcaacat acgccgtaaa atgtcggatg cgctctcgcc
     6241 gatatgctta gtgtggctgg caatatagct gtagagttca tcatcaactt caatcgtttt
     6301 catcttaatc cagtgcagtg tcttagctga atacaagtcg ccaagagtat atgaataaat
     6361 cccggcagcg gatagcgtca agcctggctt gcagaaaaac tcggaataaa ccttatttgt
     6421 tgcagccctg tggcacaatg gtcaacataa taccctaacc cgacaacgcg aaaaaaagaa
     6481 ctttgccatg aaattgaata tccgcgcgca aactgcacaa aaccagcaca ataattctcc
     6541 catcgttctt gtccatggtc tgtttggtag cctcgacaac cttggcgtac tggctcgcga
     6601 tctggtaaac gatcacaata tcatccaggt tgatatgcgt aaccacggtc tttcaccgag
     6661 agatccggta atgaattacc cggcgatggc gcaggatctt gttgatactt tggatgcaca
     6721 gcagatcgac aaagcaacat ttatcggtca ctccatgggc ggtaaagcgg taatggcact
     6781 tactgcactg gcccccgatc gcatcgataa actggtggcg atcgatatcg caccggtcga
     6841 ctatcacgta cgccgtcatg atgagatttt cgcggctatc aacgcggtca gtgaatcgga
     6901 cgcacaaact cgccagcaag cagcagcaat aatgcgccag catcttaatg aagaaggggt
     6961 gattcagttt ctgttgaaat cttttgttga cggggagtgg cgctttaacg tgccggtatt
     7021 gtgggatcag tatccgcata ttgtgggttg ggagaaaatc ccggcatggg atcaccctgc
     7081 cctgtttatc cctggcggca attctccgta tgttagcgag cagtaccgtg atgatttact
     7141 ggcacaattt ccacaggcac gggcgcatgt gattgcaggc gcgggtcact gggtccatgc
     7201 tgaaaaaccg gatgcggtat tacgcgctat ccgtcgctat ctcaatgatt aacgcaactg
     7261 attaaaaacc cagcgtccgc gtcgctttcc gcgcgtgggc gttggcgtga ctcatcgcgc
     7321 tgatgtatta tggcgcgcta tccatccggg cagaggcctg gctgattgtt tcccccgaag
     7381 tcaccaagat catggccaaa gaacaaacgg accgtacgac attagatctg ttcgcgcacg
     7441 agcgtcgacc gggacgaccg aaaactaatc cgctttcgcg cgatgaacag ctgcgtatta
     7501 ataaacgcaa ccagctaaaa cgcgacaaag tacgtggcct taagcgtgtc gaactgaagc
     7561 tgaacgcgga agctgtcgag gcgctgaacg agctggcgga gtcgcgcaat atgagccgta
     7621 gcgaactgat cgaagagatg ttaatgcagc aactggcggc gctgcgtagt cagggcatcg
     7681 tttaaatttc cactttcatg tagcacagtg tgcagtcctg ctcgtttgct gattccgcaa
     7741 gactgcctgt tctgctatga ttgcctttat ccgtgggcaa ttttccaccc ccatttcaat
     7801 aagtttcaag aggttatttc actcatggct atcactggca tctttttcgg cagcgacacc
     7861 ggtaataccg aaaatatcgc aaaaatgatt caaaaacagc ttggtaaaga cgttgccgat
     7921 gtccatgaca ttgcaaaaag cagcaaagaa gatctggaag cttatgacat tctgctgctg
     7981 ggcatcccaa cctggtatta cggcgaagcg cagtgtgact gggatgactt cttcccgact
     8041 ctcgaagaga ttgatttcaa cggcaaactg gttgcgctgt ttggttgtgg tgaccaggaa
     8101 gattacgccg aatatttctg cgacgcattg ggcaccatcc gcgacatcat tgaaccgcgc
     8161 ggtgcaacca tcgttggtca ctggccaact gcgggctatc atttcgaagc atcaaaaggt
     8221 ctggcagatg acgaccactt tgtcggtctg gctatcgacg aagaccgtca gccggaactg
     8281 accgctgaac gtgtagaaaa atgggttaaa cagatttctg aagagttgca tctcgacgaa
     8341 attcttaatg cctgatgtga tgcggcgtag actcatgtct acgccgtatt aatagataat
     8401 gccaatcaaa ataattgcta caaatttgta acttttgctg ttgtacctgt acaatgtccc
     8461 ggtgttcaag tggccttgcc gttgtaaatg taagctgtgc cacgttttta ttaacaatat
     8521 ttgccaggga cttgtggttt tcatttaggc gtggcaattc tataatgata cgcattatct
     8581 caagagcaaa ttctgtcact tcttctaatg aagtgaaccg cttagtaaca ggacagattc
     8641 cgcatgactg ataacaatac cgccctaaag aaagctggcc tgaaagtaac gcttccacgt
     8701 ttaaaaatcc tggaagttct tcaggagccg gacaaccatc acgtcagtgc ggaagattta
     8761 tacaaacgtc tgatcgatat gggtgaagaa attggtctgg ctacggtata tcgcgtactg
     8821 aaccagtttg acgacgctgg tatcgtcacc cgccacaatt ttgaaggcgg taaatccgta
     8881 tttgaactga cacagcaaca tcaccacgat cacctgattt gcctcgactg cggcaaggtt
     8941 atcgaattta gtgatgattc catcgaagcg cgtcagcgtg aaattgccgc aaaacatggc
     9001 attcgcctga ctaaccacag tctctatctt tacggtcact gtgccgaagg cgattgccgc
     9061 gaagatgagc acgcgcacga aggcaaataa gccagcctga acgagaaaag ccaacctgcg
     9121 ggttggcttt tttatacaag ggaaataaga aaagcgagag ttacagctct cacttatttg
     9181 ttattactgc gtatttcctg ccagacctta tcacaatcgg ctttcaccgc ctgatcattt
     9241 ccggtttgcg ttgcacgcag acactgctga taatccagta cgcgtacact ttcctggtcg
     9301 gcaaactgct gatgttgttt ctctttcttc agcacgttta acacgctctg gcaggcttca
     9361 attttttctg gcgaaccttg tgcggtgttg atacaggcgc tgtatgcctc tttcagacga
     9421 ctgtcttcct gtggcgcggt tgattgcgca caagctacca gccccgatgc cattatggcg
     9481 atgagaatca gttttttcat agcaaattcc tcaaacgtgc ggcaggcaca aaacctgccg
     9541 cgtcgggcat cagaagatgg tgaatggtgc gattaccata aatttcacgt cacgctcatc
     9601 ctggaagatg ttaccgtaac cgccgcccca gcttgggatg tcagagtggt tgtcgtattc
     9661 ggtgaagtgc agtttgaaca tcgtgccttt ggcgcgaccg tcctgaatgg tgtagaccgc
     9721 atccaggctg taggcagact cttcaatagt ccggtttttg tcgtagtacg cattcggatt
     9781 gctctgccag gtcgcaggtt tagcatccca tgcgtaaacg taggaagcgc cgatggcgaa
     9841 gcctggaaga ttccagtttt tcaggtcata catcgcaccg aagaagaccg ctttttcgcc
     9901 gttggcgttg aagtcggaac ggttatccca ccagatatcc aggcggccgt tggaggaagc
     9961 gtaggttgga gtcatacgtt gcaggaagta tccctgctga ccgtcagcct taacccaggt
    10021 gccttcgagg cgcaaatcca ctacgtcagc cgcccggtaa ccaaaggtca acgcttgcag
    10081 ccaggcggtg ctgtcataaa ggtcgttgac gctgcgatcg tcaactttat cgcgcgtacc
    10141 atagaactgg tagctggtgg ttaacgggct accggcgata tcaaatttgt agctggcttt
    10201 ggcaaaatat tgatcgatat acccttccgc ctgaccaaat gccgcttcca gtacgaagtt
    10261 atttttgaag tcgtatttcg ccccaaggga gtgcagataa tcaactttgg tggttttatc
    10321 gttctgataa aactcatcca tttccagatg ccacggcgct ttgtattcgt tggtccacat
    10381 gtaggagaaa ctcaacgcac cagcatcgcc gtaatcaaaa ttcgccccgg cttccgcccc
    10441 ctgataagta cctggcataa agctccagtg cggcgctaac agcgtttgac cagttggctg
    10501 aatgtaacct gccctcgccc aaaccggacc gtatttaaat ttggccgcag ctttatacag
    10561 gcttataccg cttttgtcgc cggaccagtc ttcgtcatag gctttattac tttttgaaaa
    10621 cgcgatttcg ttcgggtggg agctgtcgcc gttttccgcc atttcaatcg ccgtaaacgc
    10681 agcaatatca aggccgaaca tatcagcagc ataaccggac tgaaaatcga ggttggcatt
    10741 ccaggtggag tgagaaaggt tggttttgta tttgtcgccg tcggtaacat ctttacggtc
    10801 gcgttcacgc tgccagtaat atataccgcc ggttaaggtt gaatcgtcga tgaatccttc
    10861 ggcgcgagcc tccggcattg ccataaagcc cgacattgct gtaacaccgg cgatagccag
    10921 cgccagcgta ctacgtttgc cactaaacgt acgcatgggt taatcctctt tgacgtataa
    10981 attgctgcac caaaggtgaa cagcgaaaaa ataaaaatta aaaaagtagc gttgagaaaa
    11041 gtctcgctga ctacaggaac agactatttt tcgcgacgcg aattatcaat ctctttctgt
    11101 aaccagaatg taagaacatg agcaaccgca catattttga tgatttttgt tacaaggctt
    11161 aagtttgcgg ttgaaaggct tgcagaagaa taaaagtgtg aatttccagc aagatgaaag
    11221 cggtt
//
LOCUS       DAZGVQ010000160        11194 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000160 DAZGVQ010000000
VERSION     DAZGVQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11194
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.161"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            485..994
                     /locus_tag="ACHLAG_004199"
     CDS             485..994
                     /locus_tag="ACHLAG_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP0657329.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            966..2894
                     /locus_tag="ACHLAG_004200"
                     /pseudo
     CDS             966..2894
                     /locus_tag="ACHLAG_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            2878..3084
                     /locus_tag="ACHLAG_004201"
     CDS             2878..3084
                     /locus_tag="ACHLAG_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFP0657330.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAELVLGTTRRRRRPLGVRL"
     gene            3081..4673
                     /locus_tag="ACHLAG_004202"
     CDS             3081..4673
                     /locus_tag="ACHLAG_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0657331.1"
                     /translation="MKRKPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPSQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQ
                     LFRTRFKAVSPKRVDTPGHGIGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQGQKDNPLINILDKFSTWYDTNSVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIDSGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            4663..6168
                     /locus_tag="ACHLAG_004203"
     CDS             4663..6168
                     /locus_tag="ACHLAG_004203"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP0657332.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPEEVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMAAALNTHDTGGTMPQLTATEAAAQENQRVMGILACQEAKGREHLA
                     TMLAGQQGMSVEQARAILTAAAPQQPVASTQSEADRIMACEEAKGREQLAATLAAMPD
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEKLAAFPGMTVEAARDILS
                     SSPDKAEPVSASTTAMFERIMANHSPAAVQGGVSQTSADGDADVKMLMAMP"
     gene            6205..6552
                     /locus_tag="ACHLAG_004204"
     CDS             6205..6552
                     /locus_tag="ACHLAG_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP0657333.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISQATPALTPLMLD
                     EASGKLVVWDGQKAGSAAGILVLPLEGTETVLTYYKSGTFATEAIRWPDSVDEHKKAN
                     AFAGTALSHAALP"
     gene            6610..7638
                     /locus_tag="ACHLAG_004205"
     CDS             6610..7638
                     /locus_tag="ACHLAG_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP0657334.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFDTVEVDFGRSEGN
                     NIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7690..8073
                     /gene="gpFI"
                     /locus_tag="ACHLAG_004206"
     CDS             7690..8073
                     /gene="gpFI"
                     /locus_tag="ACHLAG_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP0657335.1"
                     /translation="MATKEENLNRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAV
                     SPEGIMHSDESGADQNHTDDGEQLNNTDAPDDVKAVRVRKCLQVMGYCPETGRPVELA
                     LRGMRVLVPSSLATAMIQHGTAEYA"
     gene            8066..8419
                     /locus_tag="ACHLAG_004207"
     CDS             8066..8419
                     /locus_tag="ACHLAG_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP0657336.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGIRAQFTSGAQRGGEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            8435..8968
                     /locus_tag="ACHLAG_004208"
     CDS             8435..8968
                     /locus_tag="ACHLAG_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0657337.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMLGRRPNSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     KELAYALKQQLRIAIKR"
     gene            8965..9360
                     /locus_tag="ACHLAG_004209"
     CDS             8965..9360
                     /locus_tag="ACHLAG_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFP0657338.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            9368..10120
                     /locus_tag="ACHLAG_004210"
     CDS             9368..10120
                     /locus_tag="ACHLAG_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0657339.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEDGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VTAIKVTQTSGTVEKGKTTTLTVSFEPESATDKTFRAVSADPSTGSIAVKDMVITVTG
                     IKAGKVSIPVISGNGQFATVAEVTVTEAGAAG"
     gene            10134..10565
                     /locus_tag="ACHLAG_004211"
     CDS             10134..10565
                     /locus_tag="ACHLAG_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP0657340.1"
                     /translation="MFLKTEQFEYNGVSVMLSELSALQRIEHLALLKRRAEEAEASGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAEE
                     VVLRLSGMSGAVHADTDSTEVAKNNALTDDDFSAGKFSTAS"
     gene            10592..11005
                     /locus_tag="ACHLAG_004212"
     CDS             10592..11005
                     /locus_tag="ACHLAG_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP0657341.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRTHYFQDTQLDMHFSGLMY
                     AVLSLFFCDPDMHPSDFSLLAPRREEAQTEMPDEEDMLMQKAAGLAGGVRFGGEGGGD
                     ISPSADVVDVSEDDVALMMASAGISGGVRYVPAGW"
     gene            10986..>11194
                     /locus_tag="ACHLAG_004213"
     CDS             10986..>11194
                     /locus_tag="ACHLAG_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0657342.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTAGAV
                     EQSLNRQALAAQKAGISVGQYKAAMR"
BASE COUNT         2620 a         2650 c         3450 g         2474 t
ORIGIN      
        1 ttgcgttacc aggatttgat aaaggcattt cagcctgaca tagccatgcg ccgtatcgtc
       61 gccgtattcc tgcattaaca gagaccgcag cccgacaggg agactcctct gcgcgagtgt
      121 gcggggataa tcaaaaacga tgcacaccgg ggtttaccgc gttaacggag cgcggcgttg
      181 tcccctcata gtcgctcgtc cggtgcgatg gtggaagaaa ctggattttg ttgcaactga
      241 taaccattat cattttctcg ggtcctttcc ggcatatgga cccgttacgg ggcggcgacc
      301 tcgcgggttt tcgctattta tgaaaatttt ccgggatcca tgtccggttt ctctgcaagt
      361 taaccatatg aaaaatataa aaacatgctt tccatgaacc ggacatgcgc aaaaaataga
      421 cactaaaacc ggacatgacc ggttttgttg tgattgtgag gtgagagttt ttgcgaggtg
      481 aggagtggct acgcagactg aagttgccag gcatttaagt ctgaccgatc gccagcttcg
      541 cagattgcag aaattgccgg gtgccccgat atcgaataag cgagggcaac tggatctgga
      601 tgcctggcgc gatttttaca tatcgtatct gagaagaagt aaaaacgatg tgcctgatgg
      661 cgatagcgaa gacgactatg aggagaaatt gcttattgcc agatgggaac tgacagcaga
      721 acaggctgtt acacagcagt taaaaaatga ggtgtcaaaa ggaaaactta ttgacaccgg
      781 gttctgtatt tttgccctca gcaagctggc aatggcgtta tccagtacgc ttgattccat
      841 ccctttatcc atgcagcgac agtttcctga tttaacaccg cgccatcttg accatctgaa
      901 aacccttatt gcgaaggggg caaatcagtg tgcgcgggcg ggggataaat taccggattt
      961 actcgatgaa tatatcagag caacaactga ataatatgat gagtgctgtc acaacagcat
     1021 tacagcccct gataagggca ttgccggtga cgccagttga atgggctgat caaaattatt
     1081 atctgcctaa agaatcttca tatggtgagg gagaatggaa aacgctgccg ttccagatcg
     1141 ccatcatgaa cagcatgggg aatgatcaga tccggactgt taatctgatt aaatctgccc
     1201 gtgttggcta tacaaagatg ttgctgggag tcgccgggta ttttattgag cataaatccc
     1261 gaaacagtct gctttttcag cccacggatt ctgccgctga agattttatg aagtctcacg
     1321 tggaggcgac gattcgcgat gttccctgcc tgaaaaaact ttctccctgg ctgggacgta
     1381 aacatcgtga taataccatc acgctgaaac gcttttcatc gggtgtgggc ttctggtgcc
     1441 tgggcggcgc tgccgccaaa aactaccgtg aaaaatccgt ggacgtggtc tgctatgacg
     1501 aactttcctc gttcgagccg gatgtcgaaa aagagggctc gccaaccctg ctgggggata
     1561 agcgtattga gggctcggta tggccaaaat ccattcgcgg ctcgacgcct aaaataaaag
     1621 gctcctgcca gatcgaaaaa gcggccaacg agtcggcaca tttcatgcgt ttttatgtgc
     1681 cctgcccgca ctgcggggag gagcagtatc tgaaatttgg cgatgagtcc acgccttttg
     1741 gccttaaatg ggagaaggac agtcccgaaa gtgttttcta cctctgtgaa catcatggct
     1801 gcgtgatcca tcagtctgaa ctggaccaga gcaacgggcg gtggatctgt gaaaacacgg
     1861 ggatgtggac ccgcgacggt ctgacgtttt tcagcgccgc ggataatgaa attccgccgc
     1921 cgcgctccat cacattccat atctggacgg cgtacagtcc gttcaccacc tgggtacaga
     1981 ttgtctatga ctggctggat gcactgaaag atcccaacgg cctgaaaacc tttgtgaaca
     2041 ccacgctggg cgagacctgg gaagaggccg tgggcgaaaa actcgatcac caggtgctga
     2101 tggataaggt tgtgcgttac acggctgcgg tgccttcccg ggtggtttat ctgacggcgg
     2161 gcattgactc gcagcgaaac cgttttgaga tgtatgtctg gggatgggct ccgggagagg
     2221 aagcctttct ggtggataaa atcatcatta tggggcgtcc cgatgaggaa gagacgctgt
     2281 tacgtgtgga tgtggcgatc aacaaaaaat accgccatgc agacggaacc gaaatgacca
     2341 tttcccgtgt ctgctgggac accgggggga tcgatggcga aattgtctat cagaggtcaa
     2401 aaaaacacgg tgttttccgg gtgctgccgg taaaaggtgc atctgtttat ggcaagccgg
     2461 tgatcaccat gccaaaaacc cgcaattagc ggggcgtgta tctgtgcgaa gtggggacgg
     2521 acaccgcaaa agaaattctc tatgcccgta tgaaagccga tcccacgcct gcggatgaag
     2581 ccacgtcgta tgccatccgt tttcctgatg atccggagat tttttcgcag acagaggcgc
     2641 agcaactggt ggcggaagag cttgtggaga agtgggaaaa aggaaagatg cgtctgctgt
     2701 gggataacaa aaagcggcgt aacgaagcgc tggactgcct ggtgtatgcc tacgcggcat
     2761 tacgtgtgtc cgtgcaacgc tggcaacttg atctggctgt actggcaaaa tcccgggaag
     2821 aagagacgac ccggccaacc cttaaagaac tggcagcgaa gctgtccgga ggagtgaatg
     2881 gttacagtcg ctgaactgca ggcgctgcgt caggcgcgcc ttgatttatt aaccggtaaa
     2941 cgggtggtgt ctgtccagaa agatggtcgc agaattgaat atacggcggc ctctctggat
     3001 gagcttaacc gggcgatcaa tgatgctgag ttggtactgg ggacaacccg ccgtcgccgt
     3061 cgtccgctgg gagtgaggtt atgaaacgaa agcctgtcct gattgatgtg aacggcgttc
     3121 cgcttcggga gagcctcagc tacaacgggg gcggtgcagg atttggcggg caaatggcag
     3181 agtggttgcc accctcgcag agtgccgatg cggccctgct gcccgcgttg cgtctgggga
     3241 atgcccgtgc agatgatctg gtgcgcaata acggaatagc ggccaatgcg gtggccctgc
     3301 ataaggatca cattgtcggg catatgtttc tgattagcta ccgtccgaac tggcgctggc
     3361 tggggatgcg ggagaccgcg gcaaaaagtt ttgtcgatga ggtggaggcg gcctggtcag
     3421 aatacgcaga agggatgttt ggtgagatcg acgtggaagg gaaacgcacg tttacggaat
     3481 ttatccgtga aggtgtgggc gttcatgcgt ttaacggcga aatctttgtg cagccggtct
     3541 gggatacgga gagcacgcaa ctgtttcgta cgcgttttaa agccgtgagt ccgaaacggg
     3601 tggacacgcc aggacacggt atcgggaacc gttttctgcg ggccggggtg gaggttgatc
     3661 gatatggccg tgccgttgcg taccatatct gtgaggatga ttttcctcgc tccgggagtg
     3721 gacgatggga acggatcccg cgtgaacttc ccaccgggcg tccggccatg ctgcatattt
     3781 tcgagccggt ggaggacggg cagacccgtg gggccaacca gttttacagc gtcatggaac
     3841 ggctgaagat gctcgattcc ctgcaggcaa cacagcttca gtcggccatt gttaaagcca
     3901 tgtatgcagc gacgattgaa agtgaacttg ataccgaaaa ggcctttgaa tatatcgccg
     3961 gtgcgccgca ggggcagaag gataatccgc ttattaatat tctggataag ttctccacct
     4021 ggtatgacac gaatagcgtg acgctgggcg gtgtcaaaat tccgcacctt ttccctggtg
     4081 atgatctgaa acttcagacc gcgcaggatt cagacaatgg attttcggcg cttgaacagg
     4141 cgctgctgcg gtatatcgct gccggtcttg gcgtttccta cgaacagttg tcccgtgatt
     4201 actcgaaggt cagttactca agtgcccgcg cctccgccaa tgagtcgtgg cgctatttta
     4261 tggggcggcg aaaatttatt gcggcccggc tggccacgca gatgttttcc tgctggctgg
     4321 aagaggcact tcttcggggg attattcgtc cgccacgggc acgttttgat ttttatcagg
     4381 cgcgttcagc ctggtcacgg gctgagtgga ttggtgccgg aagaatggcc attgacgggc
     4441 tcaaggaggt tcaggaatcg gtgatgcgta ttgattccgg actgagcacg tatgagaaag
     4501 agctggcgct gatgggcgag gattatcagg acattttccg ccagcaggtc agggaatctg
     4561 cagagcggga aaaagccgga ctctcacgtc cggtgtggat agcgcaggcg tatcagcagc
     4621 agatagcgga gagtcgcagg ccggaagagg agacaacacc acgtgagacg taatctttca
     4681 cacattattg cagcagcatt caatgaaccg ctgcttctgg agcccgccta tgcgcgggtt
     4741 ttcttttgcg cgctcgggcg cgagatgggg gcagcaagtc tttcggtacc acaacagcag
     4801 gtacagcttg atgcacccgg aatgctggct gaaacggacg agtacatggc cggaggtaaa
     4861 cgaccggccc gtgtttaccg ggtggtgaac ggtattgctg tactgccggt cacaggcacg
     4921 ctggtgcacc ggctgggggg tatgcggcca ttttccggaa tgacaggcta tgacggcatt
     4981 gtcgcctgtc ttcagcaggc aatggcggat agccaggtgc ggggtgtact gctggacatt
     5041 gacagtccgg gcgggcaggc cgccggtgcg tttgactgtg ctgacatgat ttaccgcctc
     5101 cgtcagcaga agccggtctg ggcactgtgc aatgacacgg cctgttctgc agccatgctg
     5161 ctggcgtcgg cctgctcccg acggctggtt acccagacat cccgtatcgg ctccattggc
     5221 gtgatgatga gccatgtcag ctatgccggt catctggcgc aggccggtgt ggatatcacg
     5281 ctgatttact caggggcgca caaggtggat ggcaatcagt ttgaagcgtt gccggaagag
     5341 gttcgccagg acatgcagca gcggattgat gcggcgcgcc ggatgtttgc cgaaaaagtg
     5401 gcgatgtata ccggtctgtc tgttgatgca gtcacgggaa cagaggccgc cgtttttgaa
     5461 ggtcagtccg gcattgaggc cgggctggcg gatgaattaa tcaatgcgtc ggatgccatc
     5521 agcgtgatgg ctgcggcgct gaacacacat gatacaggag gcactatgcc gcaattaact
     5581 gcaacggaag ctgccgcgca ggagaaccag cgagtgatgg ggatcctggc gtgtcaggaa
     5641 gcgaaaggac gtgaacacct tgccacgatg ctggcaggac aacagggcat gagcgttgaa
     5701 caggcccggg cgattctgac cgcggcagca ccacagcagc cggtggcatc cacgcagagt
     5761 gaagccgatc gcattatggc gtgtgaagaa gctaaaggtc gtgaacaact ggcggcaacg
     5821 ctggcggcga tgccggatat gacggtggaa aaagcccgcc cgatcctggc ggcttcaccg
     5881 caggcggatg ccggaccctc actccgtgat cagattatgg ctctggatga ggcaaaaggg
     5941 gctgaggcgc aggctgaaaa actggcggcg tttcccggaa tgacggtgga ggctgcccgc
     6001 gacattctgt cctcatcgcc ggataaagca gaaccggttt ctgcatccac aaccgccatg
     6061 tttgaacgca tcatggcgaa ccattcaccg gcagccgtgc agggtggcgt gtcacagacg
     6121 tcggcagacg gtgatgcgga cgtgaaaatg ctcatggcca tgccatgaag tcagtgctga
     6181 ccatcaatat gaggttttaa caaaatggtg acgaaaacca tcactgaaca acgtgcggaa
     6241 gtacgtattt ttgccggtaa tgatccggct cataccgcca caggcagcag cgggatttct
     6301 caggcaacac cggctctgac gcccctgatg ctggatgaag ccagcgggaa actggtggtc
     6361 tgggatggac agaaagccgg tagtgcggct ggcatactgg tactgccgct tgaaggcaca
     6421 gagacggtgc tgacctatta caagtcgggg acctttgcga cggaggcaat ccgctggcct
     6481 gacagtgtgg atgaacacaa aaaggcaaat gcctttgccg gtacagccct gagtcacgcg
     6541 gctctgccgt aacacgttat caggccacca tggtggcctg actgatttct gaatgaaagg
     6601 aactgattta tgggattgtt tacgacccgc cagttactcg gttataccga acaaaaagtg
     6661 aaattccgtg cgctgtttct ggagctgttt ttccgccgta cggtgaattt ccacaccgaa
     6721 gaggtgatgc tggacaaaat taccggaaaa acgccggtgg cagcctatgt ctccccggtt
     6781 gttgaaggaa aagtgcttcg ccatcgcggt ggtgaaaccc gcgtgttacg tccgggctac
     6841 gtcaagccca aacacgaatt taattaccag caggcggttg agcgccttcc tggtgaagat
     6901 ccggctcagc tgaatgaccc ggcctaccgt cgtctgcgta tcattaccga taacctcaaa
     6961 caggaagagc acgccattgt ccaggtggaa gaaatgcagg cggtgaatgc cgtgctgtat
     7021 ggcaaataca ccatggaagg ggatcagttt gatactgtcg aggtggattt cgggcgctct
     7081 gaaggaaata acattgagca ggctgacggt aaaaaatggt ctgagcagga ccgtgatacg
     7141 tttgatccga cgcatgatat tgacctctac tgcgatcagg ccagcggcct tgtgaatatc
     7201 gccattatgg acggtacggt ctggcgtctg ctgaatggct ttaagctgtt ccgcgaaaaa
     7261 ctggataccc gtcgcggctc aaattcacaa ctcgaaacgg cagtgaaaga cctgggggcg
     7321 gtggtgtcct tcaaggggta ttacggcgat ctggccattg tggtggcgaa aacgtcttat
     7381 gtggcagagg acggtaccga aaaacgttat ctgccggagg gcacactggt cctggggaat
     7441 acggcagcag agggcattcg ttgctatggt gccattcagg atgcgcaggc gttgtccgaa
     7501 ggtgtggtgg cctcttcccg ttatccgaaa cactggctga ctgtgggcga tccggcccgt
     7561 gaattcacca tgacgcagtc cgctccgctg atggtgctgc cggatccgga tgagtttgtg
     7621 gtggtacagg tgaaataatc cgtgagcggg ggcgaaatgc ccccgtgtct tttttcacag
     7681 gaggctgaga tggcaacaaa agaagaaaat ctgaatcgtc ttcgtcaact ggctggcctg
     7741 ctggggcgcg aggcggatat gtcggggagt gctgcggata ttgctcaacg tgtgtctgag
     7801 tgggaagagg agcttgctgt ttccccggag ggcattatgc actctgatga gagcggggct
     7861 gatcaaaatc acacagacga tggtgagcag ttgaacaaca cggatgctcc ggatgatgtt
     7921 aaagccgtcc gggtacggaa gtgcctgcaa gtaatggggt attgcccgga gacaggtcgt
     7981 cccgttgagc tggcgttacg gggtatgcgt gttctggtgc catcatcact ggcaacggcc
     8041 atgatacagc acggaacggc tgaatatgcg tgattttcag aatgcctttg atgctgccct
     8101 cgccggggtg gacagtacga tcgttgaagt gatggggatc cgtgcgcagt tcacctctgg
     8161 agcacaacgt ggcggcgaag ttcagggggt ttttgacgat ccggagtcgc tgggttttgc
     8221 cggtggcggg gtccgtattg aaggaagcag cccgtcatta tttgtgcgga cggatacggt
     8281 gcgtgccgtg cggcgtggtg acacgctgac cattaacggc gagatgttct gggtggatcg
     8341 tgtttctccg gatgacgggg gaagctgtta tctctggctc aaccgtgggc aaccacccgc
     8401 tgttaaccgg cgacgataaa cgcagggtga aattatggcg ataaaagggc ttgatcaggc
     8461 gattgacaat ctgagccggg ttcgtaaaaa cgccattccg gcggcttcag caatggccat
     8521 taaccgcgtg gccacaacgg cgattaatca gtcttcatca caggttgccc gggagacaaa
     8581 ggttcgccgg aaactggtta aggaacggtc cagactgaaa cgggccacgg tcagaaatcc
     8641 gaatgccaga attatcgtta accgcggtga tctccctgtg attaagctgg ggatcaggat
     8701 gctggggcgt cgcccgaaca gcatacttaa agccggtcag catcggtatc agcgggcatt
     8761 tattcagcga ttaaaaaatg gtcgctggca tgtcatgcag cgtgtggccg ggaaaaaccg
     8821 ttaccccatt gatgtggtga aaatcccgat ggcggcccca ctgaaacagg catttgatga
     8881 gaatgttgac cgtatccggc gtgaacgcct gcctaaagaa ctggcatacg cgctgaaaca
     8941 acaactgagg attgcgataa aacgatgaaa cacactgaca ttcgtgccgc agtgctggat
     9001 gcactcgagc agcatgaaca cggggcgacg ctgtttgatg gtcgccccgt tgtttttgac
     9061 gaagaggatt ttcctgcgat cgcggtttat ctgacggatg cagagtatac cggtgaagag
     9121 ctggatgcag atacctggcg ggccacactg catattgagg tgtttttacc ggcacaggta
     9181 cctgattcgg agctcgatca gtggatggaa agccggattt atccggcgat gactgcgatc
     9241 cctgcactgg caggactgat taccacgatg gttacgcagg gctatgagta tcgtcgtgat
     9301 gacgatatgg cgttatggag ctctgcggat ctgacttatt ccattacata cgagatgtga
     9361 ggacgatatg gcaacaccaa atcccctgga gccggtaaaa ggtgccggta ccactctgtg
     9421 ggtttacaac ggcaagggtg atgcttatgc aaacccgttg tcagacgatg actggcagcg
     9481 actggctaag gtgaaggatc tgacgccggg cgagatgacg gcagaaccct acgatgataa
     9541 ctacctggat gatgaagacg cggactggac cgcgaccggg caggggcaga agtctgcagg
     9601 agataccagt tttacgctgg cctggaaacc gggagaagat ggtcagaaag ggcttatagg
     9661 ctggtttgaa agcggggatg tgcgggccta taaaatccgt tttccgaacg gcacggtgga
     9721 tgtgtttcgt ggctgggtca gcagtatcgg taaggccgtg acggcgaaag aagtgatcac
     9781 ccgcacggtg aaagtgacca acgtgggcaa accttccgtg gcggaagaac gcagcaaaat
     9841 tacgccggtc actgcaatta aggtgacaca gacatccggt accgtggaaa aaggaaaaac
     9901 aaccaccctg acggtttctt ttgagccgga aagtgcaacc gacaagacgt tcagagcggt
     9961 ttccgccgat ccgtcaacgg gaagcattgc tgtgaaagat atggtgatca ctgtgacggg
    10021 gattaaggcc ggaaaagtga gtatccccgt gatttccggt aatggtcagt ttgccacggt
    10081 agctgaagtc accgttactg aagcgggcgc tgcagggtaa acggaggtaa tacatgtttc
    10141 tgaaaacaga acaatttgaa tataacggtg tatccgtcat gctttctgag ctgtctgcgc
    10201 tgcagcgtat tgagcatctt gccctcctga aacggcgggc agaagaggct gaagccagcg
    10261 gcaacctgca ggtgagtgtg gaagatcttg tcagaaccgg cgcgtttctg gtggcgatgt
    10321 ccctgtggca taaccatcca cagaaaacgc agtcaccgtc aatgaatgag gccgtgatga
    10381 agatagagca ggaagtgctc accacctggc ctgccgatgc cattgcccgg gcggaagagg
    10441 tggtgttacg tctgtccggg atgagcgggg ctgttcatgc ggatactgac agcaccgaag
    10501 tggcgaaaaa taacgcgctt actgatgatg atttttctgc gggaaagttt tcgacggcga
    10561 gctgaatttt gccctcagac tggcgcgtga gatggggagg cctgactggc gcgccatgct
    10621 tgccgggatg acatccaccg aatatgccga ctggcgacat ttttaccgca cgcattattt
    10681 tcaggatacc caactggata tgcatttttc cgggctgatg tacgccgtac tcagcctgtt
    10741 tttttgcgat ccggatatgc atccctcgga tttcagtctg ctggctcccc ggcgagagga
    10801 agcgcagacg gagatgccgg atgaggaaga catgctgatg cagaaagcgg caggacttgc
    10861 cggaggcgtc cggttcggtg gggagggagg gggcgatatt tcaccttctg cagatgtggt
    10921 ggatgtcagc gaggatgatg ttgcattaat gatggcttca gcggggattt ccggaggtgt
    10981 gagatatgtc ccagccggtt ggtgatcttg ttattgacct gagtctggat gctgtccgtt
    11041 tcgatgagca gatgagccgg gtaaggcgtc atttttcagg actggatacc gacgccagaa
    11101 aaaccgccgg tgccgttgag cagagtctga accgtcaggc gctggccgca caaaaagccg
    11161 ggatttccgt cgggcagtat aaagcagcca tgcg
//
LOCUS       DAZGVQ010000161        11078 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000161 DAZGVQ010000000
VERSION     DAZGVQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.162"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(229..1281)
                     /gene="fbaB"
                     /locus_tag="ACHLAG_004214"
     CDS             complement(229..1281)
                     /gene="fbaB"
                     /locus_tag="ACHLAG_004214"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFP0657343.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            1538..2815
                     /locus_tag="ACHLAG_004215"
     CDS             1538..2815
                     /locus_tag="ACHLAG_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFP0657344.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            2812..3816
                     /locus_tag="ACHLAG_004216"
     CDS             2812..3816
                     /locus_tag="ACHLAG_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFP0657345.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            3813..4778
                     /locus_tag="ACHLAG_004217"
     CDS             3813..4778
                     /locus_tag="ACHLAG_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFP0657346.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4752..5498)
                     /locus_tag="ACHLAG_004218"
     CDS             complement(4752..5498)
                     /locus_tag="ACHLAG_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP0657347.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5550..6368)
                     /locus_tag="ACHLAG_004219"
     CDS             complement(5550..6368)
                     /locus_tag="ACHLAG_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFP0657348.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFLQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFHGTVEELQAFVDGIKETP"
     gene            complement(6433..7233)
                     /gene="thiD"
                     /locus_tag="ACHLAG_004220"
     CDS             complement(6433..7233)
                     /gene="thiD"
                     /locus_tag="ACHLAG_004220"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFP0657349.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(7230..8018)
                     /gene="thiM"
                     /locus_tag="ACHLAG_004221"
     CDS             complement(7230..8018)
                     /gene="thiM"
                     /locus_tag="ACHLAG_004221"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFP0657350.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHEFLSFKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(8053..8149)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(8241..8513)
                     /gene="rcnR"
                     /locus_tag="ACHLAG_004222"
     CDS             complement(8241..8513)
                     /gene="rcnR"
                     /locus_tag="ACHLAG_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFP0657351.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            8634..9458
                     /gene="rcnA"
                     /locus_tag="ACHLAG_004223"
     CDS             8634..9458
                     /gene="rcnA"
                     /locus_tag="ACHLAG_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFP0657352.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLITVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            9677..10015
                     /gene="rcnB"
                     /locus_tag="ACHLAG_004224"
     CDS             9677..10015
                     /gene="rcnB"
                     /locus_tag="ACHLAG_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFP0657353.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(10097..>11078)
                     /locus_tag="ACHLAG_004225"
     CDS             complement(10097..>11078)
                     /locus_tag="ACHLAG_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFP0657354.1"
                     /translation="YAACYSELSVQHNLVVQGDFALTQTQMATYEHNFNDSSCVSTNT
                     ITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTSFTSGYSVTVTPAASNAKV
                     NVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGGKSWDACVNSYRNALAQNA
                     GVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGNKATINSKQGDIILRCKNL
                     LGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFILESNGSSVTLLNITNSSKG
                     YTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATALINVKYD"
BASE COUNT         2608 a         2802 c         2782 g         2886 t
ORIGIN      
        1 acgacaatgt gaaaattgtt cgaggagtaa aaggcaaaaa aaaacggcct cccgataggg
       61 aagccgtagc aaagtgcgcg tgtttttatg ccggatgcgg tgtaaacata cgtggcagtg
      121 ctatgtaggc atgataagac gcttccgcgt cgcatcaggc ataggttgcc ggatgcagtg
      181 taaacgcctt atccgtccta cggggtggtg ctgttcgtat gaggcgtatc aggcaatagt
      241 aattttgcta tcgagataaa cgtcctgcac ggcattaatc agtttcacgc cgtcagccat
      301 cgatttcttg aacgccttac gtccaagaat cagccccatt ccgccagcgc gtttgttgat
      361 aaccgcagta cgcactgcat cgctgaggtc agtttcaccg cccgcagcac cgccggagtt
      421 tatcaaccca gcccgaccca tatagcagtt agctaactga taacgcacca gatcaatcgg
      481 gttttcgctg gtcaatttgc tgtaaacacg atcgtcggtg taaccgtaat taattgcttt
      541 atagccgccg ttattttccg ccattttttg tttgacgata tctgcaccga tggttgccgc
      601 cagatggttt gcctgaccgg tcaggtcggc ggaaacatgg taatcaacgc catctttctt
      661 aaaggcggag ttacgcaaat aggcccacag cactgtcacc atacccagct cgtgcgcacg
      721 ttcaaaagcc gcagaaattt cttcaatctg gcgacgtgac tcttccgagc caaaatagat
      781 agtcgcacca accgcaaccg cgcccatgtt gaacgcctgc tccacgctgg catacagcgt
      841 ttgatcgtag gtattcgggt aacttagcgt ctcgttgtga ttaagtttga cgaggaatgg
      901 aatgcgatgc gcataacgcc gcgataccga cgccagcacg ccgtaagttg acgccacaca
      961 gttacagccc gcttcgatcg ccagttcaac aatgtttttc gggtcaaagt agagcgggtt
     1021 agcagcaaat gaagctccgg cagagtgctc aacgccctgg tcaaccggca gaatagaaag
     1081 atatcctgtg ccagccagac gcccggtgtt gtacaacgtc tgcatattac gtaacaccgc
     1141 tggcgggcga ttattgtcaa tcattacgcg gtctacgtag tcatgtccgg ggagataaag
     1201 ctggtcagaa ggaattgtca tacaacggtg ctgtaaaagg ttgtcggcgt ctttgccaag
     1261 caactgcgca atatctgtca ttactatgct cccgtaaatt ccgattggat atcggctatg
     1321 gattgtcctg gcccgccttt tgcgggcaat cataatcctg gtcgttacgg gcgagttttt
     1381 ccatcttttg gatgtttttt cagcgttttc tgttggctcg attcatcaga aaaaatgtta
     1441 gcgcggtcaa gttttcaccg caaaggtatt taaaaggtat tattaagtgg tattgtcatc
     1501 gcgtacctta cattacctgt catgaaggaa ttagaagatg aaaacaacag caaagctgtc
     1561 gttcatgatg tttgttgaat ggtttatctg gggcgcgtgg tttgtgccat tgtggttgtg
     1621 gttaagtaaa agcggtttta gtgccggaga aattggctgg tcgtatgcct gcacagccat
     1681 tgcggcgatc ctgtcgccaa ttctggttgg ctccatcact gaccgctttt tctcggcgca
     1741 aaaagtgctg gcggtattga tgttcgcagg cgcgctgctg atgtatttcg ctgcgcaaca
     1801 gaccactttt gccgggttct tcccgttact gctggcctac tcgctaacct atatgccgac
     1861 cattgcgctg actaacagta tcgcttttgc caacgtgccg gatgtggagc gtgatttccc
     1921 gcgcattcgt gtgatgggca ctatcggctg gattgcctct ggtctggcat gtggtttctt
     1981 gccgcaaatg ctggggtatg ccgatatctc accgactaac atcccgctgc tgattaccgc
     2041 cggaagttct gctctgctcg gtgtgtttgc gtttttcctg cccgacacgc cgccaaaaag
     2101 caccggcaaa atggacatta aagtcatgct cggcctggat gcgctgatcc tgctgcgcga
     2161 taaaaacttc ctcgtctttt tcttctgttc atttctgttt gcgatgccac tggcgttcta
     2221 ttacatcttt gccaacggtt atctgaccga agttggcatg aaaaacgcca ccggctggat
     2281 gacgctcggc cagttctctg aaatattctt tatgctggca ttgccgtttt tcactaaacg
     2341 ctttggtatc aaaaaggtat tattgcttgg tctggtcacc gctgcgatcc gctatggctt
     2401 ctttatttac ggtagtgcgg atgaatattt cacctacgcg ttactgttcc tcggtatttt
     2461 gcttcacggc gtaagttacg atttttacta cgttaccgct tacatctatg tcgataaaaa
     2521 agcccccgtg catatgcgta ccgccgcgca ggggctgatc acgctctgct gccagggctt
     2581 cggcagtttg ctcggctatc gtcttggcgg tgtgatgatg gaaaagatgt tcgcttatcc
     2641 ggaaccggta aacgggctga ctttcaactg gtccgggatg tggactttcg gcgcggtgat
     2701 gattgccatt atcgccgtgc tgttcatgat ttttttccgc gaatccgaca acgaaattac
     2761 ggctatcaag gtcgatgatc gcgatattgc gttgacacaa ggggaagtta aatgaaaaca
     2821 gaacgtattc tcggtgctct ttatgggcag gcgttagggg atgcgatggg gatgccctcc
     2881 gagctttggc cacgcagccg cgttaaagca cactttggct ggattgaccg ttttctgcct
     2941 ggaccaaagg agaataacgc ggcctgttat tttaaccgcg ccgaattcac cgacgatacc
     3001 tcgatggcgc tgtgtctggc ggatgcgtta ctggaacgtg aaggcaagat cgatccggat
     3061 ctgattgggc gtaatattct cgactgggcg ctgcgtttcg acgcctttaa caaaaacgta
     3121 ctaggtccga cctcgaagat tgcgcttaac gccattcgcg acggtaaacc cgttgctgaa
     3181 ctggaaaaca acggcgtgac caacggcgca gcgatgcgcg tctcgccatt aggttgtttg
     3241 cttccggcgc gtgatgttga ttcctttatt gatgatgtgg cgctggcgtc gagcccgacc
     3301 cataaatccg atctggcagt tgcaggtgcg gtagtcatcg catgggcgat ttctcgtgcc
     3361 attgacggag aaagctggtc agcgattgtt gattccctgc cttcaattgc gcgacatgca
     3421 cagcaaaaac gcatcactac cttcagcgcc tcactggcag cacgtctgga gattgcgctg
     3481 aaaattgtgc gcaatgccga cggcaccgaa tccgccagcg aacagcttta ccaggtcgtt
     3541 ggcgcaggta ccagcactat tgagtccgtt ccgtgcgcca ttgcgctggt tgaactggca
     3601 caaaccgacc cgaatcgctg cgccgtcctg tgcgctaacc ttggcggcga cacagacacc
     3661 atcggcgcta tggcgacggc gatttgcggc gcgttgcatg gcgttaacgc tatcgatcct
     3721 gcattaaagg cggaactgga tgcggtaaat cagcttgatt tcaaccgcta tgccacagcg
     3781 ctggcgaaat atcgtcaaca acgggaggcg gtatgagcgg cgctcgatta cacacgctgc
     3841 tgcctgaatt aacctcgcgt cagccggtga tggtcgtcgg cgcggcggtc attgatgtga
     3901 tcgccgacgc ttatgccctc ccctggcgtg ggtgcgatat cgaactgaag cagcagagcg
     3961 ttaacgttgg cggctgcgca ctgaatattg ccgtggcgtt aaagcgcctc ggcatcgaag
     4021 cgggtaatgc cttgccgctc ggtcagggcg tgtgggcgga gattattcgc aaccggatgg
     4081 cgaaagaggg gttaatcagc ctgatcgata acgccgaagg cgataacggc tggtgtctgg
     4141 cgctggttga gccggatggc gaacgcactt ttatgtcatt cagcggtgtg gaaaatcagt
     4201 ggaatcgcca gtggctggcg cgattaaccg ttgcgcctgg cagcctgctc tatttttccg
     4261 gttatcaact ggcctcgccc tgcggcgaat tgttagtgga atggctggaa aagctgcaag
     4321 acgtgacgcc gtttatcgat tttggcccac gtatcggcga tatcccggac gcattactgg
     4381 cgcggatcat ggcctgtcga cctttagtat cactcaatcg tcaagaggct gagattgccg
     4441 ccgaacgttt tgctttatcc gcagagataa caacacttgg caagcaatgg caggagaaat
     4501 ttgccgcgcc gttgatcgtt cgtctcgata aagagggtgc atggtatttc agcaacgacg
     4561 cttctggctg cattccggca tttccaacgc aagttgtaga caccattggg gcgggcgaca
     4621 gtcatgcagg tggcgtactt gccgggctgg cctctggtct gccactggcg gatgccgtat
     4681 tactgggcaa tgcagtggcg tcgtgggttg tcgggcatcg gggcggtgat tgtgcgccaa
     4741 cgcgcgagga actactcctc gcacacaaaa acgtatagat cgctgcgaca gtggctaatg
     4801 ctgtactcaa taggccgttg ctgttggtca agcgccactt gcttgatcac cagcaccggt
     4861 attttgctat ctaactgaat atgtgactga aactcggcat ccggcatccg ggcgctaacg
     4921 cgggaacgcg tacgttgtgg gtaaatgtgc tggctgcgga aatagtcata aagtgaaatc
     4981 ccgatggcat caacatcatg aattaaatgc gccggaaccc acgattcctc aatcgatacc
     5041 gcttcttcat cgacatagcg aatacgctta agcaaaaaga catcacttcc tgcttcgacc
     5101 gccagctgct gcgcgacttc ttcgggacat ttcacgacac gcttgttaac ccatagcgta
     5161 tcgggctttt tcccgcgcaa taccacctgc tgagaaaaac ctcgcgcttc tttcaacgag
     5221 tattcgaaga tgttgttgat ctgcgtgccg taaccgcgcg aacgcgtcac cacaccctct
     5281 tcttccagcg cctgcatcgc cttgcgcacc gtaatgcgcg aaacgccggt taactgactt
     5341 aaatcacgct caccgggcaa aatattgcca tgctccagca ccccgctgcg cacggcattt
     5401 tttaccgttt cggcaaactt aatataaagc ggcgtgttat ccgccgctaa aatacgctcg
     5461 ttcagttggg caatcagctg ggtatgcgct tgttccattt gtctttttcc tgccgaggag
     5521 tctggtggga gtatatcact gcttcttatt taaggcgttt ctttaatccc atcaacgaat
     5581 gcctgcaact cctccaccgt gccatgaaat acattaaaat ccaccggacc attgatgcca
     5641 tctacctgtc cacggtcgga atgctgccag aagcgccagg ccatgccgtc attgtccgga
     5701 cgacgttgat aatagtgagc cacccaccac ggatattcat tgaaatagcc cgccagattg
     5761 gtgtgataaa aaacggctcc tgagtaaata atcggctttt ttcccgtact tttttcgacc
     5821 atttttagcc actgacttac tcgtttgcgt aattcttttg ccgataattt tccccgttct
     5881 tctacgtcca gtaccgcagg gaaatcgcct tgtaagaaat ccaccgtttg cagaaataat
     5941 cgtgcctgaa ccggcgctgc taccgacggg gagaaataat gatacgcccc gcgcagcagg
     6001 ccattttcgc ggcttagttg ccagttacgc gaaaaatacg ggtccaccag cttttcgcct
     6061 tccgtcgcct taataaaagc aaactgtaag cggataccgt tgtcgcgcat ttttgccacc
     6121 cgctgccagt cgatccgctc ctgccagcga gaaacatcta ttccgtgaat ggtgtaactg
     6181 gcaggaatgc gaatagcgaa agatttaacc gggcgataac cgtagaaatg gatgtagtct
     6241 gtaatctgaa ctgctgtcga atagaaaaaa ttcaccgtct gacgcggata aattgcaacg
     6301 atggaaatta gcccaagagc gcataataga gaggtgagtt tttttcgact ggtaattctt
     6361 agctgcattg gtttcatccc tgaatgtcag tgccaaaggc tgacaataac caaagcagct
     6421 atagtacggt gctcaccacc aggcgtggaa gtggtgaacc ggaccaatac cgtgaccaac
     6481 ttccagcgtg tcggcctggg ctaatgctga tgaaagccag ctttttgcct cctgtacggt
     6541 gtcagcccag tttgtatggc gcgggcgtag tgcagccaac gccgcagaga gcgtacaacc
     6601 agtgccgtgg gtgtttttgg tcataatgcg cggtgcggta aaccgttgtt caccctcgcg
     6661 ggtaaacagc cagtccgggc tttgctcatc atccagatga ccacctttca ttagcactgc
     6721 gccacagccc atcgccaaca gcgatcgccc ttgttccagc atttcctgtt cggtgcgcgc
     6781 gtgtggcgcg tcgagcaagg cggcagcttc gggcaagttt ggcgttatta atgaaacctg
     6841 tggcaataat cgactgcgca gcgtagcaac cgccgaaggt gaaagcagcg ggtcgccgct
     6901 ttttgccagc ataacggtgt cgagtaccac gttttggatc tgataacgtt gcaaccgttc
     6961 tgccaccgct tcaacaatat cggtttccgc caacataccg attttagtgg tgtcgattcg
     7021 cacatcgctg aacaccgaat cgagctgggc ggcgacaaaa tcaggctcaa tgcgatacac
     7081 cgactgtacg ccacgggtat tttgcgccac cagtgcagta ataactgagc aaccataagc
     7141 gccaagtgcc gagaaggttt taagatcggc ctgaatcccc gcaccaccac tcggatcagt
     7201 accggcaatc gtcagagcgt taattcgttt catgcctgca cctcctgcgt caattgccag
     7261 agcgcatcaa ggaaatgtgg aacaaaactg cctggcccct cgcttctggc gactgcgcgc
     7321 tctccggctt gtttcatcca gtgacaggca gatgcgacat tttccagcat atcgcctggt
     7381 aaggcacaac aggcagcaac aaccgccgat aatgcacagc cagttcctac cactttggtc
     7441 attagcggat cgccaccgtg aatgccaacg atacgatgcc catcggtaac ataatccacc
     7501 tcgcctgtga ccacgacgat tgcgccagtt tcccgtgcca gtgtttgtgc agcgggtatc
     7561 gcgttagctg cggcgtcagt ggtatccact ccccgtccgc cattagcaac gccagctaat
     7621 gccatgattt ccgaagcatt accacgtatc gctgtcggtt taaaagataa aaattcatga
     7681 caaaaatggc ggcgataatc gagcgcacca accgctactg gatcaagcgt ccagggtgtt
     7741 tgagagcttt ttgcttgctc aacggcagca cgcatcgcct gtgcgcgtgg ctgcgtcagt
     7801 gtgccaacgt taatcaacaa ggcactggcg atagccgcaa actgactggc ctcttcggtt
     7861 tcgataacca tcgctggcga tgcaccgagc gccagcaagg tattggcagt aaaggtttgc
     7921 accacatcgt tggtcatgca gtgcacaaga ggggaatgtt ggtgaaaaag gtgtaacgcg
     7981 tgcgcagatt gcgctgaact cagcaggtcg acttgcatag tttgctcctg ccataacgtg
     8041 aagaagcaat gacctggtgg tccgtgactt ccctacgctg gcattatcca gatcaggtga
     8101 tacgggtatt tctcagcctt cacgcagaag ggcaccccga gtcgtttggt tgcgatgata
     8161 aatcgcagag gaggatggta atgtccagcg cacgcgttgt aaacgaggaa aaacggcagt
     8221 tttacaatcg cgttaataaa ttatttgata tatgaatcca gcaccttcag aacgacatcc
     8281 agatcttctt cacgttttag ctcatccccc tggtgaacga tgtgttccgt cagatgacct
     8341 ttaatcactt cccgcatcag accgtttacc gcgccacgga tagcagcaat ctgttgtaaa
     8401 actgcagcgc attcgtgcgg ctcgtcgagc attttcttga gcgccacgac ctggccctga
     8461 atcttactgg cacgcgcttt cagtttctgt ttatcacgga ttgtatgaga catggcaaca
     8521 cctggttaac aagaatatga aaaatcatag cactattaat ctactggggg gtagtatcag
     8581 gtactggggg ggagtagaat cagattgccg aattaatact aagaattatt atcatgaccg
     8641 aatttacaac tcttcttcag caaggaaacg cctggttctt catccccagc gccatcttac
     8701 ttggtgcgct tcatggcctg gaaccagggc actcaaaaac gatgatggcg gcgtttatca
     8761 tcgccatcaa aggcaccatt aaacaagcgg tgatgctcgg actggcagca actatttcgc
     8821 ataccgcagt ggtctggtta attgcctttg gcgggatggt gatcagcaag cgctttactg
     8881 ctcaatcagc agaaccgtgg ctccagctga tttccgcagt gatcattatt ggcaccgcgt
     8941 tctggatgtt ctggcgtacc tggcgcggcg aacgcaactg gctggagaat atgcacgagt
     9001 atgatcatga acatcatcac cacgatcacg aagatcacca cgaccatgga catcatcacc
     9061 atcacgaaca tggcgagtat caggatgccc atgcacgagc ccatgccaat gacattaaac
     9121 gacgctttga tggtagagag gtcaccaact ggcaaatttt gttatttggc ttaaccggtg
     9181 gccttatccc ctgcccggca gcaattaccg tgctgttgat atgcattcag ttgaaagccc
     9241 tgacactggg cgcaacactg gtcgtcagtt tcagcattgg cctggcgtta acgcttatca
     9301 ccgtaggcgt tggcgcagca atcagcgttc agcaggtcgc aaaacgctgg agcggattta
     9361 acactctcgc taaacgcgcc ccctattttt ccagtctgtt gattggctta gtcggtgtgt
     9421 atatgggcgt acatggcttc atgggcataa tgcgataact caatccttta ggcttgcatg
     9481 ttatcttcag cccaggatga ttcttaaatc agctatttcc gctgactctc tgctcgctaa
     9541 ggatgctttt agggcatcct ttcatttaca ctttttacga aatcatggga tcactaacaa
     9601 aatatcgctt gtcagttata ttgtatggca ggaaagatat gcgactgata ttacagatcc
     9661 ccaaagtgga gagtttatga ccattaaaaa taagatgttg ctgggtgcgc ttttgctggt
     9721 taccagtgcc gcctgggccg caccagccac cgcgggttcg accaatacct cgggaatttc
     9781 taagtatgag ttaagcagtt tcattgctga ctttaagcat ttcaaaccag gcgataccgt
     9841 cccagagatg taccgcaccg atgagtacaa cattaagcag tggcagttac gtaacctgcc
     9901 cgcgcctgat gccgggacgc actggaccta tatgggtggc gcgtacgtgt tgatcagcga
     9961 caccgacggt aaaatcatta aagcctacga cggtgagatt ttttatcatc gctaaaaaaa
    10021 gccccctcat catgaggggg aaatgcagac accttgttat tttttattat tagccacttg
    10081 ctcgtcttgc ttgttattag tcgtatttca cgttgattaa tgcggttgcc tccagtgcgc
    10141 cagatttaac tttgtttgta tcgtagacgt agtaactggc agttatcgga attgaaaccg
    10201 ttgtatctgt aagttttgac tttgccgcaa tttcttttaa atttgtgtat cctttactgc
    10261 tgttagtgat atttaaaagt gtgactgacg aaccattact ttcaagaata aatcctacgc
    10321 cattatcttc cgcacctttc agtattgtgt tgctgttggt taacaagtca gaacttgata
    10381 aatacacctg cattttccgt gatgtttgat tttgttgacc taataaattt ttacaacgca
    10441 gaataatatc cccttgttta ctatttattg ttgctttgtt acctgtggct ggtaattgtg
    10501 aaacgggaat actgtctaaa gtaattaata aatcgtccgg tttgcaggtt gtggttatcg
    10561 ggttgtatga caatgtcaga ttaaaggaat agacacctgc attttgtgcc aatgcatttc
    10621 tgtagctatt tacacatgca tcccatgact tgccacctaa taaacacagt agaaattgta
    10681 ctgcggcact cccgcgtgtc gatgatgaag cactggataa tgtcgcaact ccattaatca
    10741 tcactgaacc gccgcccccc gcagaaacat tcacttttgc attactggcc gcaggtgtca
    10801 ccgttaccga ataaccactg gtgaaactgg tctgattatt tgataacgtg atgttgtttt
    10861 tattggtcca ttcaaaatgt aaatttaatt taatggtatc gttataaagt ccaacaataa
    10921 tatccgacgg gctcataggg gtgatagtat ttgtactcac gcatgacgaa tcattaaaat
    10981 tatgctcata tgtcgccatt tgtgtttgag taagtgcaaa atccccctga acaaccaaat
    11041 tgtgctgaac agataactca ctataacatg ccgcatag
//
LOCUS       DAZGVQ010000162        11025 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000162 DAZGVQ010000000
VERSION     DAZGVQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11025
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.163"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            248..1461
                     /gene="manD"
                     /locus_tag="ACHLAG_004226"
                     /pseudo
     CDS             248..1461
                     /gene="manD"
                     /locus_tag="ACHLAG_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
     gene            1528..2811
                     /locus_tag="ACHLAG_004227"
     CDS             1528..2811
                     /locus_tag="ACHLAG_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0657355.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            3063..3980
                     /gene="oxyR"
                     /locus_tag="ACHLAG_004228"
     CDS             3063..3980
                     /gene="oxyR"
                     /locus_tag="ACHLAG_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFP0657356.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3963..5363)
                     /gene="sthA"
                     /locus_tag="ACHLAG_004229"
     CDS             complement(3963..5363)
                     /gene="sthA"
                     /locus_tag="ACHLAG_004229"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFP0657357.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5684..6334
                     /gene="fabR"
                     /locus_tag="ACHLAG_004230"
     CDS             5684..6334
                     /gene="fabR"
                     /locus_tag="ACHLAG_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFP0657358.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            6334..6693
                     /gene="yijD"
                     /locus_tag="ACHLAG_004231"
     CDS             6334..6693
                     /gene="yijD"
                     /locus_tag="ACHLAG_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFP0657359.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(6733..7833)
                     /gene="trmA"
                     /locus_tag="ACHLAG_004232"
     CDS             complement(6733..7833)
                     /gene="trmA"
                     /locus_tag="ACHLAG_004232"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFP0657360.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      7947..8137
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8202..10046
                     /gene="btuB"
                     /locus_tag="ACHLAG_004233"
     CDS             8202..10046
                     /gene="btuB"
                     /locus_tag="ACHLAG_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFP0657361.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            9991..10848
                     /gene="murI"
                     /locus_tag="ACHLAG_004234"
     CDS             9991..10848
                     /gene="murI"
                     /locus_tag="ACHLAG_004234"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFP0657362.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2531 a         2611 c         2942 g         2941 t
ORIGIN      
        1 atataattat tttctattgc tgtgcccatt ttcaatgatt aatggagttt gttatgtgat
       61 tatttaacgt gttgttttaa cgataatggt taattattca ggtggttttt gagggtattt
      121 tttctcctcg gtggaaattt ttgtttttaa tgtgatcacg gttgaataat ctctttttct
      181 ctcttgtatg gtagtatgcg taaaattaag tttgaaacgt ctatttacaa cacaggagat
      241 tgcaataatg aaaattattg ctgcagatgt ttttgttact tgcccagggc gtaatttcgt
      301 tacgcttaaa ataaccactg aaagcggact ttgtggatta ggagatgcca cgttaaatgg
      361 tcgcgaattg tcggtcgctt cttatctgaa agatcatctt tgtccacaac taattggccg
      421 cgatgccagc cgtatcgaag atatctggca attcttttat aaaggtgctt actggcgacg
      481 tggccccgtc acgatgtcag ccatttctgc cattgatatg gcgttgtggg atatcaaagc
      541 taaagcggca aatatgccat tgtatcaact gcttggtggt gcttctcgcg aaggcgtgat
      601 ggtttattgc cacaccaccg ggcggacgat tgatgaagtc cttgaagact atgcaaagca
      661 ccagcagatg ggctttaagg cgattcgtgt gcagtgcggt gtaccgggta tgcaaactac
      721 ttatggcctg gcgaagggca aaggcctggc gtatgaaccg gcaaccaaag gcctgtggcc
      781 agaagagcag ttgtggtctt cagaaaagta tctcgatttt acgccgaagt tatttgaagc
      841 ggtgcgtaat aagttcggtt ttaacgaaca tctgttgcat gacatgcacc atcgcctgac
      901 ccccatcgaa gcggcgcgct ttgggaagag cattgaggat taccgcctgt tctggatgga
      961 agatccgacg cctgcagaga accaggaatg ttttcgtctg attcgccagc ataccgtgac
     1021 gccgattgcg gtcggtgaag tgtttaacag tatctgggac tgcaagcagt taatcgaaga
     1081 gcagctgatt gactatattc gcacgacgat cacccatgca ggcggcatta ccgggatgcg
     1141 gcggattgcc gattttgcat cgctttatca ggtacgtacc ggttcgcatg gcccttcaga
     1201 tttatcgcct gtttgtcatc ggcggcgctg cattttgacc tatgggtacc aaattttggc
     1261 gttcaggagt acatgggcta ttccgaacaa atgcttgaag ttttcccgca cagctggcgc
     1321 ttcgaggaag gttatatgca cccaggcgat gaaccggggc tgggtatttc tttcgatgag
     1381 aaactggcgg cgaaatatcc gtatgacccg gcgtatctcc ccgtcgcacg actggaagat
     1441 ggcacattgt ggaactggta ataacggttc gcttaattat tttctgaata gtcatcaaat
     1501 cgattaattc cgctcaagga gctaaaaatg gacgttgatg tgtcaaccag tgttgcgggt
     1561 aataaaccgc aacggattcg tcgtattcag acagtgaccc tggttttatt atttatggcg
     1621 gggatcgtta attttctcga ccgttcgtca ttgagcgtgg caggtgaagc gattcgtggc
     1681 gagttaggat tatcggcgac agagtttggt gttttgcttt ctgcattttc tctgtcttat
     1741 ggtttttcac aactaccttc cggtattttg ctagaccgct ttggcccacg aattgtttta
     1801 ggcgcaggct taatattctg gtcattaatg caggcattaa caggaatggt taattctttt
     1861 agccacttta ttttaatgcg tatcggtctg ggtattggcg aagcgccatt tatgcctgcg
     1921 ggagtaaagt cgatcaccga ctggtatgca caaaaagaac gcggcacggc gctggggatt
     1981 tttaactcat ctaccgttat cggtcaggct atcgcgcctc ctgctcttgt attgatgcag
     2041 ctggcatggg gctggcggac gatgttcgtt atcatcggcg tggcagggat tctggttggg
     2101 atctgttggt acgcgtggta tcgcaaccgg gcgcagtttg tcctgactga cgaagaacgg
     2161 acgtatctct ccgcgccggt taaaccgcgt ccacagctgc aatttagcga gtggctggcg
     2221 ctgtttaagc atcggacaac ctgggggatg attttaggtt tctctggcgt caattatacc
     2281 ggttggctct acatcgcgtg gttacccggt tatttgcaag ccgagcaagg tttcagtctg
     2341 gcgaaaaccg gttgggtggc ggcgattcct ttcctcgcgg cggcagtcgg gatgtgggtt
     2401 aacggtattg ttgtcgatcg actggcgaaa aaaggctacg acctggcgaa gacgcgtaaa
     2461 acggctattg tctgcggttt gatgatgtcg gcattaggca cgttgctggt cgtgcaatct
     2521 tcctcgccag cccaggcggt agcgtttatc tcaatggcgc tattctgcgt gcatttcgct
     2581 ggaacgtctg catgggggct ggtgcaggtg atggtgtcag aaacaaaagt ggcttccatc
     2641 gccgggattc aaaactttgg cagttttgtt tttgcttcct ttgctccgat cgtaaccggt
     2701 tgggtagtgg ataccacaca ctcgtttaat ctggcgttgg ttatcgcggc ctgtgtgacg
     2761 ttcaccgggg cgctgtgtta cttctttatt gtcaaagatc gcattgagta atcgttacta
     2821 tcaggctctc ttgctgtggg cctgtagaat aaaaaaaagc ggatcctgga gatccgcaaa
     2881 agttcacgtt ggctttagtt attcgagttg agaaactctc gaaacgggca gtgacttcaa
     2941 gggttaaaag aggtgccgct ccgttcctgt gagcaattat cagtcagaat gcttgatagg
     3001 gataatcgtt cattgctatt cttcctatcg ccatgaacta tcgtggcgat ggaggatgga
     3061 taatgaatat tcgtgatctt gagtacctgg tggcattggc tgaacaccgc cattttcggc
     3121 gtgcggcaga ctcctgccac gttagccagc cgacgcttag cgggcaaatt cgtaagctgg
     3181 aagatgagct gggcgtgatg ttgctggagc ggaccagccg taaagtgttg ttcacccagg
     3241 cgggaatgct gctggtggat caggcgcgta ccgtgctgcg tgaggtgaaa gtccttaaag
     3301 agatggcaag ccagcagggt gagaccatgt ccgggccgct gcatattggt ttgattccca
     3361 cggtcgggcc gtacctgcta ccgcatatta tcccgatgct gcaccagacc tttccaaagc
     3421 tggaaatgta tctgcatgaa gcacagaccc accagttact ggcgcaactg gacagcggca
     3481 aactcgattg cgtgatcctc gcgctggtga aagagagcga agcattcatt gaagtgccgt
     3541 tgtttgatga gccaatgttg ctggctatct atgaagatca cccgtgggcg aaccgcgaat
     3601 gcgtaccgat ggccgatctg gcaggggaaa aactgctgat gctggaagat ggtcactgtt
     3661 tgcgcgatca ggcaatgggt ttctgttttg aagccggggc ggatgaagat acacacttcc
     3721 gcgcgaccag cctggagacg ctgcgcaaca tggtggcggc aggtagcggg atcactttac
     3781 tgcctgcgct ggctgtgccg ccggagcgca aacgcgatgg ggttgtttat ctgccgtgca
     3841 ttaagccgga accacgccgc actattggcc tggtttatcg tcctggctca ccgctgcgca
     3901 gccgctatga gcagctggca gaggccatcc gcgcacgaat ggatggccat ttcgataaag
     3961 tgttaaaaca ggcggtttaa accgtttaac gcagctaccc gataggcttc cgccatggtc
     4021 gggtagttaa aggtggtgtt gacgaagtac tcaatagtgt tgccgccacc tttctgttcc
     4081 ataatcgcct gaccgatatg aataatttcg gcagcgcgct cgccaaagca gtgaataccc
     4141 agaatctctt ttgtttcccg atggaacaaa attttcagcg tgcccacgtt catgccgacg
     4201 atttgtgcgc gtgccagatg tttaaactgg gcgcggccca cttcatatgg cactttcatc
     4261 gcggtcagct gctgttcggt tttgcccaca gagctgattt ccgggatggt gtaaatgccg
     4321 gtagggatat cttcaatcag atgcgcggtg gcttcgcctt ttaccagcgc ctgcgcggca
     4381 atgcgcccct ggtcataggc cgccgacgcc aggctcggat aaccaatcac gtcgcccacc
     4441 gcgtaaacgt gcggctgtgc ggtctgatac atgctgttga ccttcagctg tccgcgactg
     4501 tcagtttcca gcccaatgtt ctgtaacgcc agcgaatcag tattaccggt gcgaccgttg
     4561 gcatagagca ggcagtcagc tttcagtttt ttacccgact tcagatgcat gatcacaccg
     4621 tcgtcacagc cttcgatctt ctcgtactct tcgttgtggc gaatcactac gccactgttc
     4681 cagaagtgat aggagagaga atctgacatc tcttgatcga gaaatgccag cagccgatcg
     4741 cgggtgttga tcagatccac ttttacatcc ataccgcgga agatcgacgc atattcacag
     4801 ccgatcactc cagcaccata gataagtaca tggcgcggtt cgtggtgcat gctaagaatt
     4861 gagtcgctgt cgtaaatgcg tggatgggtg aaatcaacat ctgttggatg atatggacga
     4921 gagccgcagg caataacaaa tttttcagcg gttagtgttt caacgctgcc gtccgggcaa
     4981 tccagcgcca acgtatgctc gtcaacaaag cgagcgtttc cctgcaatat ttcacagtga
     5041 ttacgttcgt aaaatccctg acgcatgcgc gtttgttgat taatcacgtt atcggcatgg
     5101 ttaaggatat cggcaaaaga agagcggagc agtcgggaat ggtcgctgta aagtgggttt
     5161 tgattgaatt ctataatgcg gctgacggcg tgacggagag ctttcgacgg gatggtgccc
     5221 cagtgggtgc aaccgccgcc aacattttga taacgctcga taactgcgac gcgcgcacct
     5281 tgcttaacca ggcccattgc agcgccttcg ccgccggggc cggaacctat tactatggca
     5341 tcgtaatcgt aggaatgtgg catggtaggg cttacctgtt cttatacata aaagcaacag
     5401 aatggtaaca ttttatcgcg ggtaagccaa ttgatccccg tcatttatct ggctatatcc
     5461 tgagcggcct ttgctttgtc tgtttcttac ttttgccctg acgttttatt ggatttttat
     5521 cgacgatact ctccgtttaa gcggcaggtt tccgctgtac gtaaaagaac cggccagtaa
     5581 atatgtttta ttgcgttacc gttcattcac aatactggag caatccagta tgttcattct
     5641 ctggtatagt gccagcagta cttttggcaa ggattcagac atcgtgatgg gcataagagc
     5701 gcaacaaaaa gaaaaaaccc gccgttcgct ggtggaagcc gcatttagcc aattaagtgc
     5761 tgaacgcagc ttcgccagcc tgagtttgcg tgaagtggcg cgtgaagcgg gcattgctcc
     5821 cacctctttt tatcggcatt tccgcgacgt agacgaactg ggtctgacca tggttgatga
     5881 gagcggttta atgctacgcc aactcatgcg ccaggcgcgt cagcgtatcg ccaaaggcgg
     5941 gagtgtgatc cgcacctcgg tctccacatt tatggagttc atcggtaata atcctaacgc
     6001 cttccggtta ttattgcggg aacgctccgg cacctccgct gcgtttcgtg ccgccgttgc
     6061 gcgtgaaatt cagcacttca ttgcggaact tgcggactat ctggaactcg aaaaccatat
     6121 gccgcgtgcg tttactgaag cgcaagccga agcaatggtg acaattgtct tcagtgcggg
     6181 tgccgaggcg ttggacgtcg gcgtcgaaca acgtcggcaa ttagaagagc gactggtact
     6241 gcaactgcga atgatttcga aaggggctta ttactggtat cgccgtgaac aagagaaaac
     6301 cgcaattatt ccgggaaatg tgaaggacga gtaatgaaac aagcaaatca agatagaggt
     6361 acgctgctgc tggcgttagt tgctggctta tcgattaatg gtactttcgc agcgctgttt
     6421 agctccattg tgccattttc tgtattcccg attatttccc tggtgctgac ggtttactgc
     6481 ctgcatcaac gttatcttaa tcgcactatg ccggtaggct tgccgggtct ggcagctgcc
     6541 tgttttattc tcggcgtact gctgtacagc acggtagttc gtgcggaata tccggatatc
     6601 ggctctaact tcttcccggc agtactctcc gtcattatgg tgttctggat tggcgcgaag
     6661 atgcgtaacc gtaagcagga agttgctgag taatcggtat tatgccggat gctggcgcat
     6721 ccggcatggg ttttacttcg cggtcagtaa tacgccgcac tccatatggt gcgtgtaggg
     6781 gaactgatca aacagagcca gacgttcgac cttgtgcgtc tggcttaatg tttccagatt
     6841 cttgcataac gtttccgggt tacaggagat gtacaaaata cgcggatacg cctgcaccat
     6901 tttctcggtt tcactgtcca gaccgctgcg cggtgggtcg acaaaaatgg tttcgcactg
     6961 ataactcttt aagtcgatcc cttgcaggcg gttaaactcg cgcacaccat tcatcgcctg
     7021 agtaaattct tctgccgcca tacgaataat ttgtacgtta tcaatatggt tagctgcgat
     7081 gttgtattgc gcagcagcaa ccgacggctt agcgatttcg gtggctaata cccgatcaaa
     7141 attgcgcgcc agcgctaatg aaaagttacc gttgccgcag tacagctcca gtaaatcgcc
     7201 ttttgagcct ttggttacgt ccagcgccca ttccagcatc tgaatattca tcgccgcgtt
     7261 cggctgggta aaactgtttt ctacctgacg gtagatcatc tctttccctg cgaccggcag
     7321 acgttcatcg atgtaatctt gatccagctc gattttggtt ttcgttgccc gaccaatcag
     7381 atgcacattc agattctgcg cgcgcagtgc atcgcgcagg gcctccgctt cctgacgcca
     7441 ctcatcatcc agcttcttat ggtatagcag ggaaaccacc gcctgattac tcagcgtggt
     7501 gaggtaatca atctggaaca acttgtggcg cagaacggga ttattacgca cacccgcaat
     7561 catcgccgtc atcaactggt tgatgagttc actggcagcg gggaagctat ccacgcggat
     7621 gcggcttttg gtttgttgat cgaaaatgat gtgatacagg tcatcgccat cgtgccagat
     7681 gcggaactcc gcacgcatcc ggtaatgact gaccggcgag cgaaacactt ccggaaccag
     7741 gtcagaaaac ggtgccatca tactttgcaa acgtaccact ttttcggcta actgcgcttc
     7801 atactgttct gttggaaggt gttcgggggt catgatgtat cctgaaaaat taaagtacgc
     7861 ggcgattgta gggattgctc atcagatgtc cagatcttga tgaattccta tttgtgagct
     7921 acgtctggac agtaacttgt tacaacctgt agcatccact tgccggtcct gtgagttaat
     7981 agggaatcca gtgcgaatct ggagctgacg cgcagcggta aggaaaggtg cgatgattgc
     8041 gttatgcgga cactgccatc cggtgggaag tcatcatctc ttagtatctt agatacccct
     8101 ccaagcccga agacctgccg gccaacgtcg catctggttc tcatcatcgc gtaatattga
     8161 tgaaacctgc ggcatccttc ttctattgtg gatgctttac aatgattaaa aaagcttcgc
     8221 tgctgacggc gtgttctgtc acagcctttt ccgcttgggc acaggatacc agcccggata
     8281 ctctcgtcgt tactgctaac cgttttgaac agccgcgcag cactgtgctt gcaccaacca
     8341 ccgttgtgac ccgtcaggat atcgaccgct ggcagtcgac ctcggtcaat gatgtgctgc
     8401 gccgtcttcc gggcgtcgat atcacccaaa acggcggttc aggtcagctc tcatctattt
     8461 ttattcgcgg tacaaatgcc agtcatgtgt tggtgttaat tgatggcgta cgcctgaatc
     8521 tggcgggggt gagtggttct gccgacctta gccagttccc tattgcgctt gtccagcgtg
     8581 ttgaatatat ccgtgggcca cgctccgccg tttatggttc cgatgcaata ggcggggtgg
     8641 tgaatatcat cacgacgcgc gatgaacccg gaacggaaat ttcagcaggg tggggaagca
     8701 atagttatca gaactatgat gtctctacgc agcaacaact gggggataag acacgggtaa
     8761 cgctgttggg cgattatgcc catactcatg gttatgatgt tgttgcctat ggtaataccg
     8821 gaacgcaagc gcagacagat aacgatggtt ttttaagtaa aacgctttat ggcgcgctgg
     8881 agcataactt tactgatgcc tggagcggct ttgtgcgcgg ctatggctat gataaccgta
     8941 ccaattatga cgcgtattat tctcccggtt caccgttgct cgatacccgt aaactctata
     9001 gccaaagttg ggacgccggg ctgcgctata acggcgaact gattaaatca caactcatta
     9061 ccagctatag ccatagcaaa gattacaact acgatcccca ttatggtcgt tatgattcgt
     9121 cggcgacgct cgatgagatg aagcaataca ccgtccagtg ggcaaacaat gtcatcgttg
     9181 gtcacggtag tattggtgcg ggtgtcgact ggcagaaaca gactacgacg ccgggtacag
     9241 gttatgttga gaatggatat gatcaacgta ataccggcat ctatctgacc gggctgcaac
     9301 aagtcggcga ttttaccttt gaaggcgcag cacgcagtga cgataactca cagtttggtc
     9361 gtcatggaac ctggcaaacc agcgccggtt gggaattcat cgaaggttat cgcttcattg
     9421 cttcctacgg gacatcttat aaggcaccaa atctggggca actgtatggc ttctacggaa
     9481 atccgaatct ggacccggag aaaagcaaac agtgggaagg cgcgtttgaa ggcttaaccg
     9541 ctggggtgaa ctggcgtatt tccggatatc gtaacgatgt cagtgacttg atcgattatg
     9601 atgatcacac cctgaaatat tacaacgaag ggaaagcgcg gattaagggc gtcgaggcga
     9661 ccgccaattt tgataccgga ccactgacgc atactgtgag ttatgattat gtcgatgcgc
     9721 gcaatgcgat taccgacacg ccgttgttac gccgtgctaa acagcaggtg aaataccagc
     9781 tcgactggca gttgtatgac ttcgactggg gtattactta tcagtattta ggcactcgct
     9841 atgataagga ttactcatct tatccttatc aaaccgttaa aatgggcggt gtgagcttgt
     9901 gggatcttgc ggttgcgtat ccggtcacct ctcacctgac agttcgtggt aaaatagcca
     9961 acctgttcga caaagattat gagacagtct atggctacca aactgcagga cgggaataca
    10021 ccttgtctgg cagctacacc ttctgaacca cgtcccaccg tgctggtgtt tgactccggc
    10081 gtcggtgggt tgtcggtcta tgacgagatc cggcatctct taccggatct ccattacatt
    10141 tatgctttcg ataacgtcgc ttttccgtat ggcgaaaaaa gcgaagagtt tattgttgag
    10201 cgagtggtgg caattgtcac cgcagtgcaa gaacgttatc cccttgcgct ggctgtggtc
    10261 gcttgcaaca ccgccagtac cgtttcactt cctgcattac gcgaaaagtt cgacttcccg
    10321 gttgttggtg tcgtgccggc gattaaacct gctgcacgtc tgacggcaaa tggcattgtc
    10381 ggattactgg caacccgcgg aactgttaaa cgttcttata ctcatgagct gatcgcgcgt
    10441 ttcgctaatg aatgccagat agaaatgctg ggctcggcag agatggttga gttggctgaa
    10501 gcgaagctgc atggcgaaga tgtttctctg gatgcactaa aacgtatcct acgcccgtgg
    10561 ttaagaatga aagagccgcc agataccgtt gtattgggtt gcacccattt ccctctacta
    10621 caagaagaac tcttacaggt gctgccagag ggaacccggc tggtggattc tggcgcagcg
    10681 attgctcgca gaacggcctg gctgttagaa catgaagctc cggatgcaaa atctgccgat
    10741 gcgaatattg ccttttgtat ggcaatgacg ccagaagctg aacaattatt gcccgtttta
    10801 cagcgttacg gcttcgaaac gctcgaaaaa ctggcagttt taggctgatt tggttgaatg
    10861 ttgcgcggtc ggaaaattat tttaaatttc ctcttgtcag gccggaataa ctccctataa
    10921 tgcgccacca ctgacacgga acaacggcaa acacgccgcc gggtcagcgg ggttctcctg
    10981 agaattccga cagagaaagc aaaaataaat gcttgactct gtagc
//
LOCUS       DAZGVQ010000163        11016 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000163 DAZGVQ010000000
VERSION     DAZGVQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.164"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..243
                     /locus_tag="ACHLAG_004235"
     CDS             <1..243
                     /locus_tag="ACHLAG_004235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657363.1"
                     /translation="TGEIYKNNPYLMSGWLFLRNFNKQKINNISQRNYINSLVVLTIK
                     IAKATDRTVFAHQANIINTDLFCVIKKIDVLTLLII"
     gene            complement(369..2051)
                     /gene="glcA"
                     /locus_tag="ACHLAG_004236"
     CDS             complement(369..2051)
                     /gene="glcA"
                     /locus_tag="ACHLAG_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFP0657364.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAFISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
     gene            complement(2407..4578)
                     /gene="glcB"
                     /locus_tag="ACHLAG_004237"
     CDS             complement(2407..4578)
                     /gene="glcB"
                     /locus_tag="ACHLAG_004237"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFP0657365.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGQQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(4600..5004)
                     /locus_tag="ACHLAG_004238"
     CDS             complement(4600..5004)
                     /locus_tag="ACHLAG_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFP0657366.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            complement(5009..6232)
                     /gene="glcF"
                     /locus_tag="ACHLAG_004239"
     CDS             complement(5009..6232)
                     /gene="glcF"
                     /locus_tag="ACHLAG_004239"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFP0657367.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            complement(6243..7295)
                     /gene="glcE"
                     /locus_tag="ACHLAG_004240"
     CDS             complement(6243..7295)
                     /gene="glcE"
                     /locus_tag="ACHLAG_004240"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFP0657368.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAINEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGRALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7295..8794)
                     /gene="glcD"
                     /locus_tag="ACHLAG_004241"
     CDS             complement(7295..8794)
                     /gene="glcD"
                     /locus_tag="ACHLAG_004241"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFP0657369.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            9045..9809
                     /gene="glcC"
                     /locus_tag="ACHLAG_004242"
     CDS             9045..9809
                     /gene="glcC"
                     /locus_tag="ACHLAG_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFP0657370.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            complement(9816..10958)
                     /gene="yghO"
                     /locus_tag="ACHLAG_004243"
     CDS             complement(9816..10958)
                     /gene="yghO"
                     /locus_tag="ACHLAG_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFP0657371.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
BASE COUNT         2616 a         3068 c         2759 g         2573 t
ORIGIN      
        1 acaggtgaaa tttataagaa taacccctat ttgatgtctg gctggctatt tttacgaaat
       61 ttcaataaac aaaaaattaa caacatttca caacgcaact atataaattc gttggtagtt
      121 ttaacgatta agattgcaaa agcaacagat agaacggtgt ttgctcatca agcgaacata
      181 attaatacag acttgttttg cgtgataaaa aaaatagatg ttctcacgct cttaattatt
      241 tagcaggtta tctgaacaca aaacattatt gccgcatgga taataaagcg agaaataatt
      301 ttcaattaat aagacagaat aatgtaaatg aagccggatg atattaacga tcatccggca
      361 ttattgattt acgagaccaa cattccagta aacacatacg cctgcaacaa cgtgataacg
      421 ccgataacgc tggcaaaaat cagactgtgc ttcacggtgt agcggaatag ttcagattct
      481 cggcccacca tgcccgtcgc ggcgcaggcc acggcgatag attgcgggga gatcatcttg
      541 ccagttacgc cgccgctggt atttgctgcc accagcagag tgtcagagac gttgatttgc
      601 tgcgccgtgg tcgattgcag tgaaccaaac agggcgtttg aagaagtgtc cgagccggta
      661 aggaataccc ccagccagcc gaggaacggt gagaaaaacg ggaacatcac gcctgtacct
      721 gccagtacca gcgccaacgt ggtggacatg ccggaatagt tggtgacgaa ggcgaacgcc
      781 agcaccatgc caatcgacaa tatcggccac ttcagactaa ttagcgtttc ggcaaagacg
      841 ccaatccctt tcttgatgcc cacgccgagg atgaagatag agataaacgc cgcaataaaa
      901 atagcggtgc cgccagccga gagagggtcg aatttaaaca ccgcatccat tggcgttggt
      961 tgggcgacaa tgggtgccgc tttcaacact tgttgatgca aatgagggat ctggaaatta
     1021 atcaccagtg aataaaacgc gccgcccgga gcaaataacg ctttaaacgg cttcatggtc
     1081 cagatggtga ccagcaccgt taagattaaa aacggtgacc acgcacgaat gatttgcccc
     1141 agactatatt ctgaaggcac gggaccgcca gaagatggct tatttaccac catcgcacct
     1201 gcggattgcc ccatgctgat tgccgtttcg gtatttttcg gccgccagac tttaaggaat
     1261 aaagcgagtg agacgatact caccagcgcc gaagtaatat ccggcagttc cggaccaata
     1321 tagttagagg taaagaactg agtgacagca aagcttcccc cagcaaccag cgccgctggc
     1381 cacgtctctt tcaccccttt ccagccgtcc atcattgcta ccagccagaa cggcacaaga
     1441 accgacagga acggtaactg acgtcccgcc attgcgccaa tgtggaacgg atcgattccc
     1501 gttacctgac cggcgaccag aatcggcacg cccaacgcac caaacgccac tggcgcagtg
     1561 ttggcaatca gacacagccc cgccgcgtat aacggtttga agcccaggcc caccagcagc
     1621 gcaccggtaa tcgccaccgg cgcaccaaag ccagccgctc cttccagtaa cgcaccaaag
     1681 gagaaaccaa tcagtaacac ctgcaaacgc tgatcgtcgg tgatggagat aaccgagctg
     1741 cggataatat cgaactgacc gctggcaacg gttaatttat agaggaacac cgccgcgaca
     1801 ataatccacg caataggcca taatccataa ataaagccat agcccgcagc agcaaaagcc
     1861 atatcaatcg gcattttaaa ggcgaatatt gcaatcagga tagataatat aagggttatt
     1921 gctccagcga catgtccttt cagacgtaat accgcgagtg caacgaagaa gaatattatc
     1981 gggatcaggg cgaccagagc ggatagcccc agtcctccca tcggcatata catttgggtc
     2041 caggtaacca tattgttcag tctcttatta tctttatatg cttgatatac ttaaggttgt
     2101 aataagcaaa agaggactga actgtaaaat ataggcgtta tactttacag caacagtacg
     2161 ccgctaacgc aattgctacc tctggcataa caagtatatc gggtaagggt ttctgttctg
     2221 cacacgcagg cgcagagtat cgttaagatg tccatattgt tgttataggc ccgctagtaa
     2281 tgcgctacgg gtatttaata ttgttaaacc ctgataatcg ctccggttat ttccgggata
     2341 aatgtactac cgcagttact atcatagccc cgacaataaa acttgccggg gctttttttg
     2401 acgctattaa tgactttctt tttcgcgtaa acgccaggcg tgtaataacg gttcggtata
     2461 gccgtttggc tgtttcacgc cgaggaagat taaatcgctg gcagctttaa aagcacacga
     2521 gttagcgaaa ttccccgcca tcggacgata agccggatcg ccagcgtttt gctgatcaac
     2581 cactttcgcc atattctcca gcgacgcctg cacctgttct ttggtcagaa taccgtgacg
     2641 taaccagttg gcgatatgct ggctggagat acgcagcgtt gcgcggtctt ccatcagcgc
     2701 cacattgtga atatccggca cttttgaaca accaatcccc tgctccaccc agcgcaccac
     2761 gtaccccaga atcccctgca cgttgttatc cagctcttgt tggatctctt ccaccgacca
     2821 gttagcgttt tcagccaccg gaatagtcag cagatcgtcc agcagcggtt caaattcagc
     2881 attgaactcg gtctgggcaa tgttggcttg cacgctctgt acgttggttt ggtggtagtg
     2941 cagcgcatgg agcgtagcag cggttggtga cggaacccag gctgtgtttg ccccggcacg
     3001 cagttggtcg cccttctggc tgtacatgtc tgccatcagg tccggcattg cccacatgcc
     3061 tttaccaatt tgcgctttac cgcgcagccc acagaacaga ccggaaagca cgttattacg
     3121 ctcgtaggct ttgatccacg gcgtcgattt catctggttt ttacgcagca tcgggccagc
     3181 ttccatcacc gaatgcattt catcgccggt acggtcgagg aaaccggtat tgatgaacgc
     3241 cacgcggttg cgcgcctgag cgatacagct acgcaagttc agcgaggtcc gacgttcttc
     3301 atccataatg cccattttca gggtattcgg tgccataccg agcattgtct caatgcgggt
     3361 aaacagtttg ttggcgaacg ccacttcctg cggaccgtgc attttcggtt tcacaatata
     3421 gacgctgcca gtgcgcgagt ttttctgcac ttttaaatca tagagggcaa tcgcgccagt
     3481 catgacgcca tcaagaatgc cttccgggat ttcattgcct tcgctgtccc aaatcacagg
     3541 aatggtcatc aaatgaccca cgttgcggat aaacagcagc gagcgtccgt gcagagaaat
     3601 ttcagagcca tcggcggcgg tgtaatgacg atcgtcattc agtttacgca cgatttgctg
     3661 accgtttttc tccattttct cttgcagagt cccctgcatc aggcccagca ggttgcggta
     3721 cagcaggatt ttatcttccg catcaaccgc cgcgaccgaa tcttcgcagt cgagaatggt
     3781 actgatggcc gcttcgacga taacatcgtt gatgtgcgcc ggatcgtctt tgccaatccg
     3841 cccgttggca tcaatttgca gttcaatatg caggccgtta tttttcagca aaatgcaggt
     3901 cggcgcagcg gcatcgccac ggtaaccgac aaactgcgcc ggggtacgta acgtggtttc
     3961 tttaccattt ttcaactgga tgcgtaattg tttatcgacc accttaaacg ccaccacatc
     4021 ctgatagctg ccgttttcca gcggtagaga ttcatcgagg aaacgccgaa cccaggcgat
     4081 aacctgctca ccgcgttgcg gatcgtagcc gctgaccatc gccccttcct gcgggatgat
     4141 gtcgctgccg tataacgcat cgtacagtga gccccagcga gcgttcgccg cgttcagcgc
     4201 gtagcgggcg ttcattgccg gaaccaccag ctgtggcccc gcctgactgg tgatttcgct
     4261 gtcaatgccc gtggtttcca ccgtcacgcg ctccggttgc ggcaccaggt agcccagttc
     4321 acgcaggaaa gatttatagg ccgctttatc ttttaccggc cccggattgc tgcgatgcca
     4381 ctcatcaagc gccgcctgaa tgcgatcgcg ttctgccagc aactgacgat tttctggtgc
     4441 cagatcgtga acgatttcat caaaattgcg ccagaacgcc gcagcgtcca gccctgttcc
     4501 cggtaaaact tcttcatcca caaaacgttt aaaattggcg tcaatgcgta aacggccctg
     4561 ggttatggtt tgactcattg tttatctcct cgttttcgct tatttcgcca acaccgctgc
     4621 tgccgctttc gcgacctgcg catcctgtgc tccggttaaa ccagaaacgc ccacggcacc
     4681 aataatttgc ccatcaacaa caaccggtac gccaccttcc agcgacgtca gtaacggcgc
     4741 agtcacgaac gcggtacgtc cgttgttcac catctcttca tagcccttag tttcacgacg
     4801 ccccagcgcg gcggtacgcg ctttctcctg ggagatgtaa gccgcaatcg gcgcgcaatc
     4861 gtccatgcga cttaacgcca gcagatgacc accgtcatcg gcaacagcaa tggaaacaga
     4921 ccagttattt ttctgcgcct cttcctgacc tgcggcaata attgcactcg ccatttgctg
     4981 gctaagaatg actttagttt tcattttttt attccttttc aagggcttgt tctacaattt
     5041 caatccagtg acgcacagag gtacgaccgg cgctcgccag atgcgtctgg caaccaatgt
     5101 tggcggtgac gatcatttcc ggtttgccgc tttccagcgc attcattttg ttatcccgca
     5161 gctggcgtgc cagatcggga tgcgttaacg catatgttcc cgctgaaccg cagcacagat
     5221 ggctgtcggg aacgtccgtt aaggtaaatc caagacgaag caacactttt tccacttcgc
     5281 cgttcagctt ttgcgcatgt tgtagggtac acgggcagtg gaaggccagt tttttatcgc
     5341 cgcgaactgc cagtttttcc agcggttcct cgcgcagaag ctcgactaaa tcgaccgcca
     5401 gttcactgac ttgacgcgct ttatcggcat ataacgcatc gtttttcagc atctgcccgt
     5461 actctttgac aaacgcgccg caaccgctgg cggtttgcaa aattgcctcg gcacctgctt
     5521 caatcgcggg ccaccaggca tcaatattat tgcgcgcccg tgccagccct ttctcctgcg
     5581 cattaagatg atagtccacc gcgccacaac agcctgcttc gttagctgac atgacgctga
     5641 tccccagacg atccagcact cgcgcagttg ccgcgttggt gttgggcgaa agcgtaggct
     5701 gggcgcagcc ttccagcatt aaaacccgac gcttatggcg cagcggcgga cgcggtttag
     5761 ctttcaccgt ttcagcaggc agttttgctc tgacctgttc cggtaaaaac ggtcgcagca
     5821 ccagccctac ctgcgtcagc gcacggaaga ccgccggacg cggcactacc tggcgcaatc
     5881 cttcgcgcag cattcgctcc ggcagtgggc gtttcacttt ctgctcgaca atatcacgcc
     5941 cgatatccag caaattgtga tagcgcacac cagaaggaca ggtggtttca caattgcggc
     6001 aagtgaggca gcgatcgaga tgctcctgtg ttttaagcgt gacttcgttg ccttccagca
     6061 cctgtttaat cagatagatg cgcccgcgcg gcccgtccag ttcatcgccc agaagctgat
     6121 aggttgggca ggttgcggta caaaatccgc agtgaacaca ggcgcgcagg atgctgtcgg
     6181 cttccagcgc gcgcgcgttc tgccgcatct cttcagttaa ttgggtttgc atagcctgct
     6241 cctcaaagtt ccgcgtacat gcgaccgggg ttaaacacgc cgcaagggtc gagctgctgt
     6301 ttaagctgct ggtgatagcg gaataaagga gccgatagcg gggcaaagcc accatctccg
     6361 gcactaaagc gggtcgcatg accgccagcg ttgcgggcga tgcgatggat ttgattgtcc
     6421 tcggctgtcg atttcagcca gcgtaacgct ctgccccagt cgatcagttg ctcgccgggt
     6481 aaatccatca tcggcgcatc gctgggtaat gaaatgcgcc ataaggtacc tggtaacgag
     6541 aagaacggca gttgttgttc acgcaattgc tgccagaact gacccgcaac ctcttcgccc
     6601 cccagcagtt cacgcgccgc ttttaccgat ccttcgccgc cctcaaggcg gatccacaac
     6661 gcattgtcga agtaacataa gccactaatg ggtagcggct ggagttgcca ctgggcgatt
     6721 tcgttaatgg cttcttgcag gctgatttcc cgacgcaggc tcagggaggc gcgcggtcgc
     6781 ggtaacactt tcatcgagat ttcagtgagc acgccaagac aaccgtagct tccggccatt
     6841 aaccgtgaga gatcgtatcc ggcaacgttt ttcatcactt cgccaccaaa acgcagatgt
     6901 tttccagcgc cggtaatgat gcgcgtgccg aggacaaaat cgcggaccga accgctccac
     6961 gggcgacgcg gccccgccag cccgcaggcg accatcccgc cccaggtggc ttcttcacca
     7021 taatgcggcg gctcgcaggg gagcatttgc ccggcgctct ccagcgccgc ttcaatcgcc
     7081 accagcggcg ttccggcacg cgcggttatc accagctcgg tcgggtcgta attaacaatg
     7141 ccgcgatgac aacgcacatc cagcgtttgc ccggtgacag ggcgacctaa aaaggctttg
     7201 ctattgctgc cctgaatcac cagcggcgtt ttatcgctaa ttgcctgatt cacctgctcc
     7261 agcagcgcct ggctgtaatc acactcgcgt agcatcagaa acgctccagt tcagggaaag
     7321 gtaaatgacc gtgatgcaca tgcatggcac caaattcagc acagcggtgt agcgtgggaa
     7381 tgtttttccc agggttcagc aaaccatcgg ggtcaaacgc cgccttgacc gcatggaagg
     7441 tcgtgatttc atcgctgttg aactgggcgc acatttgatt gattttttct cgcccgatgc
     7501 catgttcccc actgatgctg ccgccaactt caacgcagag ttcgaggatc ttcccaccca
     7561 gctcttccgc gcgggcaaat tcaccgggtt cgttggcatc gaaaaggatt aacgggtgca
     7621 tgttgccatc tccggcatga aagacgttgg caacacgtaa atcatattgc tgcgataaac
     7681 gggcaatgcc ttccagtacg ccaggcaggg cgcgacgcgg gatggtgcca tccatgcagt
     7741 agtaatccgg ggagatacgt cctaccgccg ggaacgcatt tttgcgaccg gcccagaaac
     7801 gtacgcgctc tgcttcgtcc tgtgccagac ggacgtcagt cgcgcccgct ttcaacaaga
     7861 tgtcgttaac ccgctcgcag tcttcctgta cgtcagactc cacgccgtcc agctcgcata
     7921 acaaaatcgc ttcggcgtcg acgggataac cggcatgaat aaaatcttcc gccgcgcgga
     7981 tcgacaggtt atccatcatc tccagcccgc cggggataat gccattggcg atgatgtcac
     8041 caactgcaag tccggctttt tctaccgagt caaagctggc taacagaacc cgcgccacgg
     8101 gcggcttcgg cagcagtttt accgtcactt cggtggtcac gccgagcata ccttccgatc
     8161 cggtgaacag cgccagcagg tcaaaaccag gtgaatccag cgcgtccgat cccagcgtca
     8221 gcgcctcgcc gtccagcgtt tgcacttcaa ttttcagcag gttatgtacg gtcagaccat
     8281 atttcaggca gtggacgccg ccggcatttt ctgccacgtt gccgccaatg gaacaggcga
     8341 tttgtgagga agggtccggt gcgtagtaga gattatgcgg tgcaacggcc tgggagatcg
     8401 ccaggttacg cacgcctggc tgcacgcgcg cgcggcgacc aacggggtta atgtcgagga
     8461 tctctttaaa gcgcgccatc accaacaaca cacctttttc cagcggcagc gcgccaccag
     8521 aaagcccggt gcctgcacca cgggtcacca ccggtacacg caggcgatgg cagacagcaa
     8581 gaatcgctgt cacctgttcc atttgcttag gcagaacaac cagtaatgga cgcgtgcgat
     8641 acgcgctcaa cccgtcacac tcgtaaggaa tgatctcctc atcggtatgc aggatctcaa
     8701 gtccagggac atgctcacgc agtgccatca gtaccgatgt gcggtcgaca tcgggtaaag
     8761 cgccatcaag acgctcttcg tacaagatgc tcatgagtag gcttcgcttt gttgtgttgt
     8821 gtggcagctg atttttgcgc gctgcttctg tgaacagtta ttaagcgggc ttttcgtttt
     8881 cgtctatctc tttagctacc ggtcagacca ttttttttcc agctctgtga ccttgccttg
     8941 gttaactcaa tgttaaattg atgtaacata atcacttacg tgatgtgcgt gttttgcgag
     9001 ttaagaacag aaaaattggt cctacctgtg cacgaggtcc gggaatgaaa gatgaacgtc
     9061 gccctatttg cgaagtggtt gcagagagta tcgaacggtt aattatcgac ggcgtactga
     9121 aggtcggtca gccacttccc tcggaacgtc gactgtgtga aaagctcggc ttctcacgct
     9181 ccgcactgcg tgaagggctg accgtgctgc gcgggcgcgg gattattgaa acggcgcagg
     9241 gtcgcgattc tcgtgtcgca cggcttaatc gggtgcagga caccagcccg ctgatccatc
     9301 tgttcagtac gcagccgcga acgctgtacg atctgctcga cgttcgcgca ttactggagg
     9361 gcgaatcggc aaggctggcg gcaacgctgg gaacgcaggc tgattttgtt gtgataaccc
     9421 gctgttatga aaaaatgctc gccgccagtg agaacaacaa agagatttcg ctgatcgaac
     9481 atgcgcagtt ggatcacgct ttccatctcg ccatttgtca ggcttctcac aatcaggtgc
     9541 tggtgtttac gctgcaatca ttgaccgatc tgatgtttaa ttcagtgttt gccagcgtaa
     9601 ataatctcta ccatcgacca cagcaaaaaa agcagatcga tcgccagcat gcgaggatct
     9661 acaacgcggt gttgcagcgg ctgccgcacg tcgcccagcg cgcagcacgc gatcatgtgc
     9721 ggaccgtgaa aaagaatctc cacgatatcg agctggaagg ccaccatttg attcgctcgg
     9781 cggtgccgct ggagatgaac ctgagttagc tggtattaaa tctgcttttc atacaatcgg
     9841 taacgcttgt acggctccgc cccaatgcgt tccagcatgt tattcatgcc tgtattggtt
     9901 tcgaggatcc atgacatctc cagcgcatcg atcttccggc gggcaaacgg atcgcgtaag
     9961 gcttcaatca ataacagcgc aatcaccggg ccgatgcggc tgaactgata ctcgtcgcgc
    10021 acgcccatca gcggtactcg cgcagttcgc acaccgctga ctttcaaacg ccacagcaat
    10081 tttgcccagc cgaagggaaa gagcgatccg ttcagatcgg caatcgcctc gttgatgttc
    10141 ggcaagccga caataaacgc gcagggtgca gaatcaatct cagcgatata gatcatatcg
    10201 tccggcacca gatatttaag ttgatcgccc atggtcgcga attcatgttc ggtaaacggc
    10261 acaaatcccc agttgtgctg ccagccagag ttgaaaatct cacgcaggat ctgcatttct
    10321 tcggcaaacc gctgacgatt gatgcagcga atggtcacct ttttgcgcac ctgatccatc
    10381 agttttttta gcgccggaga gaaagtgaga tcggttcgct gcatccacca cgccagtaaa
    10441 tcaatgcctt tgtgataacc cagttgttca atatgcgcgg cataccacgg tttgccgtgt
    10501 ggcatcatcg cacagggtgg tgtgtcaaaa ccttcaatca gtaatccgct ttcctgattg
    10561 atattcaggc tgaaaggacc gctgatctta cttgcacctt gtgacttcaa ccacgcttcc
    10621 gctgcgccaa acaacgcggc aaaaacctgc ggatcatcaa tggcgtcaat catgccgaaa
    10681 tgaccggtat cttcaccgta acgctcacgg tgcaaggtat ctatttgcgc ggtaatacgc
    10741 ccaactatct gccccgcttt ttttgccacc cacgcctgcc agatgatatg gtccgtcccc
    10801 ggatttttcg cagacaaatg ctcgttgcgt tctatgaata aagggggtat ccagtttgga
    10861 tcgtcgggat aaagtgatga cggaaaagcg ataaatgcct taaggtcatt tttattaatt
    10921 actttttcaa ttttaatcat cagcagatcg cattccatat ctctgtggat aacaaataat
    10981 taacggtgaa aaacaggttg caacagcaac gaacga
//
LOCUS       DAZGVQ010000164        10964 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000164 DAZGVQ010000000
VERSION     DAZGVQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.165"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            105..719
                     /gene="evgA"
                     /locus_tag="ACHLAG_004244"
     CDS             105..719
                     /gene="evgA"
                     /locus_tag="ACHLAG_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFP0657372.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            724..4317
                     /gene="evgS"
                     /locus_tag="ACHLAG_004245"
     CDS             724..4317
                     /gene="evgS"
                     /locus_tag="ACHLAG_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFP0657373.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4373..5518)
                     /gene="yfdE"
                     /locus_tag="ACHLAG_004246"
     CDS             complement(4373..5518)
                     /gene="yfdE"
                     /locus_tag="ACHLAG_004246"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFP0657374.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5592..6536)
                     /gene="yfdV"
                     /locus_tag="ACHLAG_004247"
     CDS             complement(5592..6536)
                     /gene="yfdV"
                     /locus_tag="ACHLAG_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFP0657375.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFRGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFIVTAPLWIYVSRLVS"
     gene            complement(6606..8300)
                     /gene="oxc"
                     /locus_tag="ACHLAG_004248"
     CDS             complement(6606..8300)
                     /gene="oxc"
                     /locus_tag="ACHLAG_004248"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFP0657376.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8354..9604)
                     /gene="frc"
                     /locus_tag="ACHLAG_004249"
     CDS             complement(8354..9604)
                     /gene="frc"
                     /locus_tag="ACHLAG_004249"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFP0657377.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10117..10749)
                     /locus_tag="ACHLAG_004250"
     CDS             complement(10117..10749)
                     /locus_tag="ACHLAG_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFP0657378.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNNDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         3211 a         2460 c         2264 g         3029 t
ORIGIN      
        1 aggaagctca gattttctta tttttattga gaaaatgaga tgacgcctta tgtctgtatt
       61 actacaggga gaagggagat gcttcattgc aaagggaata atctatgaac gcaataatta
      121 ttgatgacca tcctcttgct atcgcagcaa ttcgtaattt attgatcaaa aacgatattg
      181 aaatcttagc ggagttgact gaagggggaa gcgccgttca gcgggtggaa acacttaagc
      241 ctgatatcgt catcattgat gtcgatatcc ccggagttaa cggtatccag gtgttagaaa
      301 cgctgaggaa gcgccaatat agcggaatta ttattattgt ctccgctaaa aatgaccatt
      361 tttacgggaa acattgtgct gatgctggcg ctaatggatt cgtgagtaaa aaagaaggca
      421 tgaacaatat cattgcggct attgaagctg caaaaaatgg ctactgctat ttccccttct
      481 ctctcaaccg gtttgttgga agtttaacgt ccgaccagca aaaactcgac tccttatcga
      541 aacaagaaat tagtgtcatg cggtatattc ttgatggcaa ggataataat gacattgctg
      601 aaaaaatgtt catcagcaac aaaactgtca gcacttataa aagtcgcctg atggaaaaat
      661 tagaatgtaa atcactgatg gatctttaca cattcgcgca acgtaacaaa atcggctaat
      721 cacatgaagt ttttacccta tttttttctt ctctgttgtg gtctttggtc gaccataagt
      781 ttcgcagacg aagattacat cgaatatcgt ggcatcagta gtaacaaccg tgtcacactt
      841 gatccagtac gtctgagcaa caaggaatta cgttggttag cgagcaaaaa aaatcttgtg
      901 attgcagtac acaagtccca aacggctacc ttgttgcata ccgattcgca gcaacaggtt
      961 cgtggtatta atgctgatta tttaaatctt ttaaaaagag cgttaaatat caaattaaca
     1021 ctccgggaat acgcagatca tcaaaaagca atggacgcgc tggaagaagg tgaagtcgat
     1081 atagtgttat cacatttagt tacttcgccg cctcttaata atgacattgc tgcaaccaaa
     1141 ccattgataa ttacctttcc ggcgctggta accacccttc acgactcaat gcgaccgctt
     1201 acctcaccaa aaccagtaaa tattgctcgg gtagcaaatt accccccaga cgaggtaatt
     1261 catcaatcat ttccaaaagc aacaattatc tcttttacaa atttatatca ggcattagca
     1321 tccgtctcag ctgggcacaa tgattacttt attggtagta acatcattac cagcagtatg
     1381 atttcccgct atttcactca ctccttaaat gtagtgaaat attataactc gccgcgtcaa
     1441 tataattttt tcttgaccag aaaagaatct gtcattctta atgaagtact caatagattt
     1501 gttgatgctt taacaaatga agttcgctat gaagtatcac aaaattggct tgatacagga
     1561 aacctggcct ttctgaacaa accattagaa ctcactgaac atgaaaaaca gtggattaag
     1621 cagcatccca atttaaaggt gctggaaaat ccttactcgc ccccctattc tatgacggat
     1681 gaaaatggct cggttcgggg cgttatgggg gacattctta atattattac cttgcaaaca
     1741 ggtttaaatt tttctccgat caccgtttca cacaatatcc atgctggaac acagcttagc
     1801 cccggaggat gggatataat acctggcgct atttatagtg aagatcgaga aaataatgtt
     1861 ttatttgctg aagccttcat aacaacgcct tacgtttttg tcatgcaaaa agcgcctgac
     1921 agtgaacaaa cattaaaaaa aggaatgaaa gttgccattc catattatta tgagctgcat
     1981 tcgcaattaa aagagatgta tccggaggtt gaatggatac aggtcgataa tgccagcgct
     2041 gcatttcaca aggttaagga aggtgaactt gatgctctgg tcgcgacaca gttaaattcg
     2101 cgttacatga tcgatcatta ctatcctaat gaactttatc attttcttat tcctggcgtt
     2161 ccgaatgcat cgctttcgtt cgcttttcct cgcggagaac cggaacttaa ggatattatt
     2221 aataaagcac tgaatgcaat tcccccaagc gaagttctgc gcctgacgga aaaatggatt
     2281 aaaatgccca atgtgaccat tgacacatgg gacctatata gcgagcaatt ttatattgtt
     2341 acgacattat ccgttttatt agttggcagt agccttttat ggggattcta cctgttacgc
     2401 tcagttcgtc gtcgtaaagt cattcagggt gatttagaaa accaaatatc attccgaaaa
     2461 gcactctcgg attccttacc gaatccaact tatgttgtaa actggcaagg taatgtcatt
     2521 agtcataata gtgcttttga acattatttc actgcggatt actacaaaaa tgcaatgtta
     2581 ccattagaaa acagtgactc accctttaaa gatgtttttt ctaatgcgca tgaagtcaca
     2641 gcagaaacga aagaaaatcg aacaatatac acacaggtat ttgaaattga taatggcatc
     2701 gagaaaagat gcattaatca ctggcataca ttatgcaatc ttcctgcaag tgacaatgca
     2761 gtatatattt gtggttggca agatattact gaaacgcgtg atctaattaa tgcactcgag
     2821 gtagaaaaaa ataaagcgat aaaggctacc gtagcaaaaa gtcagtttct ggcaacgatg
     2881 agtcacgaaa taagaacacc aataagctct attatgggct tcctggaact tctgtcgggt
     2941 tctggtctta gcaaggagca acgggtggag gcgatttcac ttgcctacgc caccggacaa
     3001 tcactcctcg gcttaattgg tgaaatcctt gatgtcgaca aaattgaatc gggtaactat
     3061 caacttcaac cacaatgggt cgatatccct actttagtcc agaacacttg tcactctttc
     3121 ggtgcgattg ctgcaagcaa atccatcgca ttaagttgca gcagtacatt tcctgatcat
     3181 tacctggtta agatcgatcc tcaggcgttt aagcaggtct tatcaaattt actgagcaat
     3241 gctctcaaat ttaccaccga gggggcagta aaaattacga cctccctggt tcacattgat
     3301 gacaaccacg ctgttatcaa aatgacgatt atggattctg gaagtggatt atcgcaggaa
     3361 gaacaacaac aactctttaa acgctacagc caaacaagtg caggtcgcca gcaaacagga
     3421 tctggcttag gcttaatgat ctgcaaagaa ttaattaaaa atatgcaggg cgatttgtca
     3481 ttagaaagtc atccaggcat aggaacaaca tttacgatca caatcccggt agaaattagc
     3541 cagcaagtgg cgactgtcga ggcaaaagca gaacacccca tcacactacc tgaaaagttg
     3601 agcatattaa tcgcggatga tcatccgacc aacaggctat tactcaaacg ccagctaaat
     3661 ctattaggat atgatgttga tgaagccact gatggtgtgc aagcgctaca caaagtcagt
     3721 atgcaacatt atgatctgct tattactgac gttaatatgc cgaatatgga tggttttgag
     3781 ttgactcgca aactccgtga gcaaaattct tccttaccca tctgggggct tacagccaac
     3841 gcacaggcta acgaacgtga aaaagggtta agttgcggca tgaacttatg tttgttcaaa
     3901 ccgttgaccc tggatgtact gaaaacacat ttaagtcagt tacaccaggt tgcgcatatt
     3961 gcacctcagt atcgccacct tgatatcgaa gccctgaaaa ataatacggc gaacgatcta
     4021 caactgatgc aggagattct catgactttc cagcatgaaa cgcataaaga tctacccgct
     4081 gcgtttcaag cactagaagc tggcgataac agaactttcc atcagtgtat tcatcgcatc
     4141 cacggtgcgg ctaacatcct gaatttgcaa aagttgatta atattagcca tcagttagaa
     4201 ataacacctg tttcagatga cagtaagcct gaaattcttc agttgctgaa ctctgtaaaa
     4261 gaacacattg cagagctgga ccaggagatt gctgttttct gtcagaaaaa tgactaaata
     4321 gcggctccca caatgttcaa atgtgggagc tatttaccag cacatcttac atttatgatg
     4381 agaactcctg gcgaatttgt tccccatgct ggtcgagcgt tgccgctccc ggcataacat
     4441 gcgggtccgc gcagccgctg atttttatcg gattacccgg catcattatt cccccggctt
     4501 caatcaacat atttctcgcc tgagtttgtg gcaatttaat ggcctcagcc acacttaata
     4561 acggcgcgac gggtacacca acttcatgta ttctggctaa ccaaacttca gctgcctgcg
     4621 tttttaacgt ccgctcaata tattgtttaa gaatagcctg gttttgtacg cgtaaaatat
     4681 tgctgctaaa tcggggatca ttaaccagtt ccgtaagctc cagtgcctgg cataacgcag
     4741 aaaaaagctt gtcattacca caacaaatcg taatcggctt atcctgagta ttgaaaacat
     4801 caaaaggtgc catgtaggga tggcgatttc ccagacgttg tggtgacttc cctgtcgcga
     4861 tatatgccat cagaccatgc tccagaaaac tcagcgtggc atcaaacatc gctatatcga
     4921 catgcgcccc tctctggctc ttttcgcggc cataaagtgc actcactatt ccgctgaata
     4981 aatagacacc gccgcataga tccgcaagag atgtaccaac gcgcactggc ggagcatcag
     5041 gatatcctgt ttccatcatt atcccgctca ttgcctgaat gatggtatcg taggcaggag
     5101 catcttttag cggaccggta tgtccgaaac ctgacgatga agcatatatg aggcgcgggt
     5161 tgatttcttg aagcgtttcc catgaaaacc ccagtttttc cattgtacct gggcgaaaat
     5221 tctcagctaa tacatcagct tgtttgagca tatttataaa tatactttta tcgtgatcat
     5281 tctttaaatc aagaaccaca ctctctttgc catgattaat aaaactgtaa tagagtgact
     5341 gtccatccac atagggacca aatgtgcggg tatcatcacc atgacccggc ggctcaactt
     5401 taattaccct tgcgcccata ttacaaagaa gttgagttcc gaaaggtcca ttaaggacat
     5461 gtgtcatatc gataactaat aagccttcaa acggcccttt gctttcatta tttgtcattt
     5521 gcttatgcct tgaaaaaagc cgaaggtgaa aagacacttc ggcatttatg taaaatcaat
     5581 aacatgatta attatgaaac cagtcgactg acataaatcc acaagggagc cgtgacgata
     5641 aagcccagta cgctcacagc caatgacgct gtaccagtac gggtgtagac attaaaccgg
     5701 ctggcaataa tgattccgga gaatgccggc ggtaatgcgc ctgccagtac catcatctgc
     5761 agatgttcgc tgttcaaatg acatgccata ccaacgagaa gcagtgccag tggcatcaga
     5821 atcagcttca ggaaggtgtt ataagcaatt tcggcactga actcgaattt atgtgcagcc
     5881 agagtcaacc ccgcagcgaa taccgctacc cctgagttag ccttcgcaat cagattaaag
     5941 gttgggtccc atgctgccgg aatttttacc ccaaccaaca ccaggatcgt tgccagaaca
     6001 ggtgcccata ctactggctc ctttgccgca gaaattaatg cgctcagatt actattcttc
     6061 ttaccatccg ctcctgagga aggattcagc aaatacagac caataggaat agtaattgcg
     6121 ttaacaataa tagaaataat tgctaccact aaacctgttg atacggaatc accataaata
     6181 ggatcgagaa ctgcaaaccc caggaatcca atggtaggtg aacccgcaat taatgcacat
     6241 acagctgctt ctgcatgggt acgtttaaaa aatttgtagc aaccaaacca ggagaaaaag
     6301 aaacatccga caataaccac aagtgataca agggtcagac gagtgtccgc aaaaatcatt
     6361 tccctgtttg cccgggtaat agaaacaaat agagccgcag gaagagcgta gtttaatacc
     6421 agtttattaa atgcgcgggc ttgatcttct gaaaaagttt ctcgtctgcc tctaaaataa
     6481 cccaacagca tgattacgat aataggcaat aaatcgccaa taaaaaatgt tagcataata
     6541 gtatttccga taagggtccc ctcaccatcg cataatgagt tggcaagagg agacgatgtc
     6601 agtatttaat taccagcgac ttgttttggg ttaagtttgg taatatggcc actttcagtt
     6661 cctgctgcag ggtcgatgac cacattaata atggtcggtt tgcgcgactg aataccggtg
     6721 gttaaagcat gacgaagttc atctgtcgtg gtgacgttat agccaacgcc acgaaacgca
     6781 tccattaatt tgtcatacct tgcatggtgc aacagatccg ttggtgatgg tgcaccagcg
     6841 ccactgagat caacaccgtc tcctctgtag atgccgccat tattaaaaat aacgatcgtc
     6901 accggcaggt tatatcgaca aatcgtttca atttccatcc cactgaaacc aaaagcacta
     6961 tcaccttcaa tggcgacaac cggagaacca gaagtcacgc tagcaccgat ggcatagccc
     7021 ataccgatgc ccatgacacc ccaggtacca caatccagac gacgacgtgg tttatacata
     7081 tcaataatat ttcgtgcatt atccagggta tttgcacctt cattaactaa ataaatatcc
     7141 tggttctcgc gcaatacgtc gcgcacagca cttaatgcat taaagtaatt taatggttga
     7201 gtatctgtac ttaatttttc atgcattttt tgtgcatttt gctgcttgtg gatatttaaa
     7261 atatcgcgcc ataccagtgg agtcgtaaat gtgttttgtt tcagttctgc cagcatacct
     7321 tgcatactgg atgcaatatc accaacgact ggcacagcaa tggggcggtt gctgtcaatt
     7381 tcctgcggtt caatatccag ttgaataaac tgtgtatctg ccgcccatcc ttttttaccg
     7441 tgtgccaata accaattcag tcgtgcacca acaagcatga caacgtcagc atttgccagg
     7501 gcaaacgaac gcgcagctgc cgcagaaagt ggatgcgtat cttcaaggat ccctttcgcc
     7561 atagacattg gcaggaatgg aatctgagta ctttcaataa attcacgaag ctgttcatca
     7621 gcttgtgaat acgccgcgcc tttgccaagg ataattaatg gccgttcagc ttttgctaaa
     7681 agcgaaattg cgctagtgac tgacttcggg catggcaata atgctggcga tggattttca
     7741 actttaacaa tcgtggttaa cgcttcgtct ttttccatcg tcgcggccag gacatttgct
     7801 ggcaaatcaa gataaactcc gccagggcga cccgatacag agacccggat agcgcgtgcc
     7861 aatgcaatgc caagatcctg cggctgatta acgcgaaatg ctgctttggc atacggtttt
     7921 gccgcattca tttggtccag ctcttcataa tcaccttgct gtaggtcgac gatcgcgcgg
     7981 tcgctggagc cgctaatcat gatcatcgga aaaccattta ccgttgcgtt ggccaatgcg
     8041 gtcaaaccat tgaggaatcc tggcgcagaa actgtcaggc agatccccgg tttttgggta
     8101 agaaaaccgc ttgccgcagc ggcatagcct gccgactgct catgacgaaa accaatataa
     8161 cgaatgcctt ccgcctgggc atggcgtgcc atatccgtca caggaatacc tacaacacca
     8221 taaatagtgt caatattatt ctgttttaat gcttcaacga tgatatgcat accatctgtc
     8281 atttgaagtt gatctgacat gattaataac ctcgaaattt ttataaattg ggagccagaa
     8341 gcccccgtta atatcagatg gcgtggtttt gcttcattgc agcaatttca tcgtcgctat
     8401 aacccagctc ctgcaataca gcagcggtat gttcacctaa tagcggcgca gctttaatat
     8461 ccggcgtaaa ggcagagaat ttcattggac aaccaacggt cagatatttt ccacgcaacg
     8521 gttgttccac ttcaacaaca ctgccacttt ggcgcaaaga gggatcaagt gaaatttctt
     8581 tcatacttaa aaccggtgca caaggaatat caaactgagt caaataggcc accgcttcat
     8641 gtttatcaat agtgacagtg tatttttcga tttcagcaaa aatatcgaaa atatgtggct
     8701 gtcgggcatg ggctgtactg tatgccggat cggtaatcca ttctggtttg ccgatggctt
     8761 tacaggtgtt ttcccagttt tgctcctgaa tagtgaaata aatataggcg ttaggatcgg
     8821 tttcccagcc tttacatttc aggatccagc caggctgacc gccaccacct gcattaccac
     8881 cgcggggaac tgcatcacca aatgtaccat tcggatactg cgggtattct tccagataac
     8941 ccaatttatc gagacgctgc tggtcacgta atttcacgcg gcaaaggttc aatacggcat
     9001 cctgcattga catggtgact cgttgcccac gccccgtttt ttcgcgatgc agcaaagcag
     9061 caagtaaacc gatcagcaaa tgcattccgg tgttgctgtc acccaacgct gcagcgctta
     9121 ccagcggcgg accatcccaa aaacccgtag tggatgccgc gccacccgct gcctgagcaa
     9181 cgttttcata ggcttttaca ttcacataag gcgaacactc atcaaaccct ttgatcgaac
     9241 caaaaatcag acgtggattg atttcttgaa tatgctccca ggtgaagccc atgtgatcaa
     9301 tggcccctgg atgaaagttc tcgactaaga tatcagcttc gcggatcagc ttttccatta
     9361 cctctttgcc ttccgctgtt ttggtattta actcaataga acgtttgtta ctgttaagca
     9421 tggtgaagta aagcgcatcg atatcaggaa tatctcgcag ctggtgacgc gttacgtcac
     9481 caacgccggg acgttcaatt ttaataacgt cagcgccaaa ccaggccagc atttgagtac
     9541 aagatgggcc agattgcaca ccggtgaaat cgagaacttt aattccttga agtggagttg
     9601 acatagcaat acctattagt taattaaata tgtgttaatt tagcgtttat ttaaggcgta
     9661 atatcttcta taaataaccc gcaaaatatc gtcgtagctg cactcgatat ttttaaaatc
     9721 ctgtatcaag tgctgaaaat catttcatcg ccacttccaa ttcctttctg catggtaagt
     9781 agtgaggacg aaaagaagaa tatgataaat attgattcgt ggtaatcaga gagagtcgcg
     9841 acatttgtga tgttattctt acaactgtaa gaagcgccgg tgatttacaa gtaagggtat
     9901 gattacgctt tttaaagcgt accataaaac ataacataat aaatgatttg tgatgacacg
     9961 ggattattgg gatttacttt gaggaagata aaatgcgtgt ttatagaatc gcgagcgcca
    10021 aataatttta tgaccacaac ctgtcgtatt gtggtcataa aaaattgtcg gctggaggga
    10081 gttaaatcct ttacctcaat atactgcctg tcagcattag ttcacaaaca gtgcttcgct
    10141 gtcgacgatg attccatctt ccgcaccttt aagcgccagg tttgcctcgt agtattgctt
    10201 tttatcaagc agtttctgcg catcagcaag tgcattacga gtctgtttaa gcggcatcag
    10261 atattggttt tccattacgc caacaccggc caggcgaagt tcttccattg cgcccttttt
    10321 atcccctttc gccatttttt cattggcaat ttttatcgcg gcttcttttt cgggagttgc
    10381 tacataactc tcagaaattc cgacagatgc attaatgaca atatattggt cattattgag
    10441 attggtcttt ttacctggct tagcgaactt tgcccattcg gtactgtcat ctgacagcaa
    10501 agcggaagct tcattggtca gttcttttgc cttttctgga tcgccatgaa acagagccag
    10561 gcgcgctacc tgcacgtcac gcatcgcata taaaccttgt tcagagatac gctctgctgc
    10621 tgccgttttc tgggtttgct gaacttgctg agtttgctgt tgtgccgcta ccggtgcatt
    10681 atcagcagcc catacagttg aagatgccag aattgctgtg accatcgtag ccataattaa
    10741 acgtttcata cttacctcat tctttaacag ttttagaaaa cgccgccatc atattgggtg
    10801 agcatgaaat gagaaatcag agagcaacgg tcattttgaa agggttatct tacagttgta
    10861 gttattatcg caagaagtat tttagtctta caattgtagg aatctgcttc ctgtcgtaga
    10921 gaactattct tatgtaccac aaagagatgt catttattat atcg
//
LOCUS       DAZGVQ010000165        10955 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000165 DAZGVQ010000000
VERSION     DAZGVQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.166"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(28..1857)
                     /gene="glmS"
                     /locus_tag="ACHLAG_004251"
     CDS             complement(28..1857)
                     /gene="glmS"
                     /locus_tag="ACHLAG_004251"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFP0657379.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2019..3389)
                     /gene="glmU"
                     /locus_tag="ACHLAG_004252"
     CDS             complement(2019..3389)
                     /gene="glmU"
                     /locus_tag="ACHLAG_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFP0657380.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3740..4159)
                     /locus_tag="ACHLAG_004253"
     CDS             complement(3740..4159)
                     /locus_tag="ACHLAG_004253"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFP0657381.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4180..5562)
                     /gene="atpD"
                     /locus_tag="ACHLAG_004254"
     CDS             complement(4180..5562)
                     /gene="atpD"
                     /locus_tag="ACHLAG_004254"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFP0657382.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5589..6452)
                     /gene="atpG"
                     /locus_tag="ACHLAG_004255"
     CDS             complement(5589..6452)
                     /gene="atpG"
                     /locus_tag="ACHLAG_004255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFP0657383.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6503..8044)
                     /gene="atpA"
                     /locus_tag="ACHLAG_004256"
     CDS             complement(6503..8044)
                     /gene="atpA"
                     /locus_tag="ACHLAG_004256"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFP0657384.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8057..8590)
                     /gene="atpH"
                     /locus_tag="ACHLAG_004257"
     CDS             complement(8057..8590)
                     /gene="atpH"
                     /locus_tag="ACHLAG_004257"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFP0657385.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8605..9075)
                     /gene="atpF"
                     /locus_tag="ACHLAG_004258"
     CDS             complement(8605..9075)
                     /gene="atpF"
                     /locus_tag="ACHLAG_004258"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFP0657386.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9137..9376)
                     /gene="atpE"
                     /locus_tag="ACHLAG_004259"
     CDS             complement(9137..9376)
                     /gene="atpE"
                     /locus_tag="ACHLAG_004259"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFP0657387.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9423..10238)
                     /gene="atpB"
                     /locus_tag="ACHLAG_004260"
     CDS             complement(9423..10238)
                     /gene="atpB"
                     /locus_tag="ACHLAG_004260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFP0657388.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQHPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10247..10627)
                     /gene="atpI"
                     /locus_tag="ACHLAG_004261"
     CDS             complement(10247..10627)
                     /gene="atpI"
                     /locus_tag="ACHLAG_004261"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFP0657389.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2601 a         2998 c         2721 g         2635 t
ORIGIN      
        1 gcagcccttt tttatctatg atagtgatta ctcaaccgta accgattttg ccaggttacg
       61 cggctggtca acgtcggtgc ctttgatcag cgcgacatgg taagccagca gctgcagcgg
      121 aacggtgtag aagatcggtg caatcacctc ttccacatgc ggcatctcga tgatgtgcat
      181 gttatcgcta cttacaaaac ccgcatcctg atcggcgaag acatacaact gaccgccacg
      241 cgcgcgaact tcttcaatgt tggatttcag tttttccaac agttcgttgt tcggtgcaac
      301 gacgataacc ggcatatccg cgtcaatcag cgccagcggg ccgtgtttca gctcgcctgc
      361 agcgtaggct tcagcgtgaa tgtaagagat ctctttcagt ttcaatgcgc cttccagcgc
      421 gattgggtac tgatcgccac ggcccaggaa cagcgcgtga tgtttgtcag agaaatcttc
      481 tgccagagct tcaatgcgtt tgtcctgaga cagcatctgc tcaatacggc tcggcagcgc
      541 ctgcagacca tgcacgatgt catgttcaat ggaggcatcc agacctttca ggcgagacag
      601 cttcgccacc agcatcaaca gcacagttaa ctgagtggtg aatgctttag tggatgccac
      661 gccgatttcc gtacccgcgt tggtcatcag cgccagatcg gattcgcgca ccagagaaga
      721 acccggaacg ttacagattg ccagcgaacc aaggtaaccc agctctttcg acagacgcag
      781 gcccgccagg gtatccgcgg tttcgccaga ctgtgacaag gttatcatca ggctgttacg
      841 acgcacggca gatttgcgat agcggaattc agaggcgatt tcgacgtcgc acggaatacc
      901 tgccagcgat tcaaaccagt aacgggaaac cataccggag ttataagaag taccacaggc
      961 gaggatctga atatgctcaa ccttcgacag cagttcgtcg gcgttcggtc ccagctcgct
     1021 taaatcaacc tgaccgtggc tgatgcgccc ggtaagggtg ttttttatcg cgttcggctg
     1081 ttcgtagatc tctttctgca tgtagtgacg gtaaatacct ttatcgcccg cgtcatattg
     1141 cagattggat tcgatatcct gacgttttac ttccgcgcca gttttatcga agatgtttac
     1201 cgaacggcga gtaatttccg caatatcgcc ctcttcaagg aagataaagc gacgggtcac
     1261 cggcaacagc gccagctggt cagaagcgat aaagttttcg cccataccca ggccaatcac
     1321 cagcggacta ccagaacgtg ccgccagcag ggtatccggg tgacgggagt ccatgatcac
     1381 tgtaccgtac gcaccacgca gttgcgggat agcacgcaga acagcctcac gcagagtacc
     1441 gccctgtttc agctcccagt tcaccagatg ggcaattact tcggtgtctg tttcagaaac
     1501 gaaggtatag ccacgcgctt ttaactcttc gcgcagcggt tcatggtttt cgatgatgcc
     1561 gttatgcacc accacaatgt gttcagaaac atgcggatgc gcattcactt ctgaaggttc
     1621 accgtgggtc gcccagcgag tatgagcaat acccgtaccg ccatgcagag gatgttcttc
     1681 cgctgcctga gccagcatct ggactttacc gaggcgacgc aggcgggtca tatgaccttc
     1741 cgcatcaaca acggccagac cggcagagtc atatccgcgg tattccagac gacgtaaacc
     1801 ttcaagaagg atttctgcta catcacgttg cgcgatcgcg ccaacaattc cacacatagt
     1861 ttttgattcc gatttatatc gttgtcggtc aacctgtatg cccgtttttt cgggcgcccc
     1921 gagccttgta gagagtgggg ttatttttat agttactgct tgtgggaggg agattatgtt
     1981 atctcctcat cccatgtgac cggattaccc ggccagaatc actttttctt taccggacga
     2041 cgccagcctt ctttctgagt ctgcggcaca cggctgatag ctaatgcatt ttcgccgaca
     2101 ttacgcgtca cagttgtacc cgcagcaatg gtcgcgcctt tgcctactgt taccggggcc
     2161 accagctgag tgtcggaacc gacaaacaca tcgtcgccga taatggtctt aaatttattc
     2221 gcaccatcgt agttgcaggt aatggttccc gcgccgatgt taacgttatc gccaatttcc
     2281 gcatcgccca ggtaagtcag atgaccagct ttcgagcctt tacccagacg cgcttttttc
     2341 atctcaacaa agttaccgac gtgtgcacct tccagcaact cagcaccagg acgcagtcgg
     2401 gcaaacgggc caatagtaca ggccgctgcc agattcgcgt cttccacaac ggtatacgga
     2461 ctgatttcgc aatcatcgcc aatcacgctg tttttaatca cgcaaccggt gccgattttc
     2521 acgcgatgac cgagagtcac gttgccctcg atgataacgt tagtatcaat ttcaacatcg
     2581 cgcccgtgag taagcgtacc gcgcaaatca aaacgcgccg gatcgcgcag cataacgcct
     2641 gctaacagca gtttttcagc ctgttcggac tggtaaacgc gctccagacg ggagagttgc
     2701 aggcggttat tcacaccttc tacttcgctt aaacgttgcg gatgaacggc gacgatttca
     2761 cgcccttcct gatacgccag cgcaataatg tcggtgatgt agtattcgcc ctgagcatta
     2821 ttgttggtca gcttcgccag ccagcgtttc atatctgcgc cgttagcgat cagaatgccg
     2881 gtgttgatct cctgaatctg acgctgctcg tcggtggcat ctttgtgctc aacaatgccg
     2941 gtaactttgc cattttcacg ggtgatacgt ccataaccgg tcggatcatc cagtttcacc
     3001 gtcagcagac caatgccacc ctgcggttta gcatcacgca ggcgctggag tgtttcgaca
     3061 gagatcagcg gcacgtcgcc gtagagcatt aaaatgtctt catcatcggc aaagaaaggt
     3121 gcggcctgct gcattgcatg acccgtaccc agctgctctg cctgaagcac ccagttaagg
     3181 ttgtcgtctt tcagcgcctg tttaagcaga tcgccgccgt gaccgtacac caggtgaacg
     3241 tgcgctgcgc ctaattcatt cgcagcatca atgacatgct gaaccatcgc tttcccggca
     3301 agggtatgca gcactttcgg aagatcggaa tacatgcgcg tgcctttgcc tgcggcaagg
     3361 atcactacgc tcatagcatt attcaacata cgcgtcctga ctgtaatttg agaacgaatt
     3421 taaaccgctt caccttgaaa aaactacatt tttttcatcg tgaaatggac agaggataaa
     3481 ttgttcaata acggattatc cccgctgacg aaagtgccat tttcgaccat cgtcacgcct
     3541 tttgtctgca gaaaattaag gcagtacctt catttaagcg tcaggtggat gtttttgctc
     3601 ttatttcgat caatgaataa acgggaaaac aggggtgttt tatatcagca ggatctatgt
     3661 gaacgctctt caggacggat cacgcgtaaa aaaaagccag cctgtttcca gactggcttt
     3721 tgtgcttttc aagccggtgt tacatcgctt ttttggtcaa ctcgataacg cgcagctgcg
     3781 cgatcgcttt ggccagttcc gcagacgcct gagcgtaatc tacgtcgccg tgagagctgc
     3841 taatgtgctc ttcagcctta cgtttcgctt ccatggctcg cgcttcgtcg agatcctggc
     3901 cgcgaattgc ggtgtcggcc agaacggtca cgttgccagg ctgcacttca agaatgccgc
     3961 cagacagata gataaactct tcgtgaccgt gctgtttcac gatgcgaatc ataccaggct
     4021 taatggcggt gagcagcggt gcgtggccag ggtagatccc cagttcacct tcgctacccg
     4081 ttacctggat tttctcgacc agaccagaga acatttgttg ctctgcgctg acgacgtcca
     4141 ggtggtaagt cattgccata tcaccctccg attaaggcgt taaagttttt tggctttttc
     4201 cacagcttct tcgatggaac cgaccatgta gaacgcctgc tccggcaggt gatcgtattc
     4261 gccttccatg atgcctttaa agccacggat ggtgtctttc agggagacgt atttacccgg
     4321 agaaccggtg aatacttctg ccacgaagaa cggctgggac aggaagcgct ggatcttacg
     4381 agcacgcgct accaccagtt tgtcttcttc agacagttca tccataccca ggatggcgat
     4441 gatgtctttc agttcctgat aacgttgcag gatggactga acgccacgcg cggtgtcgta
     4501 gtgttcctga ccaaccacca gcgggtccag ctgacggctg gtggagtcca gcgggtcaac
     4561 ggccgggtag atacccagag acgcgatctg acggctcagt accacggttg cgtcaaggtg
     4621 cgcaaaggtg gttgccggag acgggtcagt caagtcatcc gcaggtacgt atactgcctg
     4681 tacggaggtg atagaaccag ttttggtgga ggtgatacgt tcctgcagaa cgcccatctc
     4741 ttccgccagg gtcggctgat aacctaccgc tgaaggcata cggcccagca gtgcggatac
     4801 ttccgtacct gccagggtgt aacgatagat gttgtcaacg aacagcagaa cgtcacgacc
     4861 ttcgtcacgg aatttctcag ccatggtcag accggtcaga gcaacgcgca gacggtttcc
     4921 cggcggctcg ttcatctggc catacaccag ggatactttg tcgataacgt tggagtcggt
     4981 catttcgtgg tagaagtcgt taccctcacg agtacgttca cctacgcccg caaacacaga
     5041 gtaaccggag tgctcgatcg cgatgttacg aatgagctcc atcatgttta cggttttacc
     5101 tacacccgca ccaccgaaca gaccaacttt accgccctta gcgaacggac acatcaggtc
     5161 gataactttg ataccggttt ccagcagttc ctgagagttt gacagctctt cgtaggaagg
     5221 tgctgcgcgg tgaatcgccc aacgctcttc ttcaccgatc tcgcctttca tgtcgaccgg
     5281 ttcacccagt acgttcatga tacggcccag agtcgcttta cctaccggga cttcgatcgg
     5341 gtgttcgagg tcttttacat ccagaccgcg acgcagaccg tcggaggaac ccattgcgat
     5401 ggtacgcacg ataccgccgc cgagctgctg ctgaacttcc agcaccagac gctcattacc
     5461 attttgcacc tcaagagcat cgtacacgcg cggtacggca tcctgaggga attcgacgtc
     5521 aactacggcg ccgattacct ggacaatctt tccagtagcc atcttaaatc ctctacgaaa
     5581 taacctgttt aaaccgcggc ggcccccgag acgatctcgg tgagttcctg agtaatgctg
     5641 gcctgacgag ctttgttgta taccaactgc agctctttaa tcaggctgcc gccattgtcg
     5701 gtcgcggctt tcatcgccac catacgggcg gcctgctcgc tggccaggtt ttcaaccacg
     5761 ccctgataaa cctgagattc gacataacga cgcagcaggg tatccagcaa cgccttcgga
     5821 tcgggttcgt acaggtaatc ccaggattta tgtttcagat catcatcatc tgatgccggt
     5881 aacggcagca gctggctgat ggtcggaacc tgagacatgg tgttaataaa tttgttgctg
     5941 acaatgtaaa gcttgtccag acggccttcg tcgtaggcct gcaacatcac ttttaccgga
     6001 ccgatcagtt cggacaggga agggttatcc cccatgccgg tgacctgggc aacaacattg
     6061 ccgcccacgg agttgaagaa cgacacgcct ttcgagccga tcattgcgag gtcgcattga
     6121 acgcctttgt cggtccaggt cttcatttcc gccagcagtt ttttgaacag gttaatgttc
     6181 aaaccaccgc acaaaccacg gtcggtcgac accaccaggt agcccacgcg tttaacgtcg
     6241 cggtcttcca ggtaagggtg cttatattcc agattaccgt gtgcaaggtg accaatcact
     6301 ttgcgcatgg tttctgcata aggacggctg gccgccatgc gatcctgcga tttacgcatt
     6361 ttggaagcgg cgaccatctc catcgcttta gtgatctttt gcgtgttctg gacgcttgcg
     6421 atcttactac gtatctcttt tgcgccggcc atgagcttct cctcaatgcc ttgcggcctg
     6481 ccctaaggca agccgccaga cgttaccagg attgggttgc tttgaaggaa tcgaggatgc
     6541 ctttcagctt gccttcgatt tcgtcgttgt agccaccggt ctggttgatc tcttgcatca
     6601 acggagcgtg atcacggtcg acgtaagcca gcagagcggc ttcgaagctg ccaattttcg
     6661 acagttcaac atccgccagg taaccacgtt ctgctgcgaa cagaaccaga gactgctgcg
     6721 caacggacat cggcgcatac tgtttctgtt tcagcagttc ggtcactttc tgaccgtggt
     6781 caagctgctt acgtgttgca tcgtcaaggt cggatgcaaa ctgagagaac gctgccagtt
     6841 cacgatactg tgccagagcg gtacggatac caccggacag ttttttcatg atcttggtct
     6901 gtgctgcacc accaacacgg gatacggaaa tacccgggtt aaccgcagga cgaataccgg
     6961 cgttgaacag gttggtttcc aggaagatct gaccatcggt aatggagatt acgttggtcg
     7021 gaacgaacgc agaaacgtca cccgcctgag tttcgataat cggcagtgcg gtcagagaac
     7081 cggttttccc tttcacttca cctttggtga aggcttcaac gtattcggcg ttaacacgtg
     7141 cagcacgctc cagcagacga gagtggaggt agaaaacgtc gcccgggaat gcttcacgtc
     7201 ctggcggacg acggagcagc agggagatct gacggtaagc aacagcctgt ttagacaggt
     7261 catcgtaaat gatcagcgca tcttcaccgc ggtcacggaa gtattcgccc attgcgcaac
     7321 cggcatacgg tgccaggtat tgcagtgcag cggattcaga cgcggttgct accacaacga
     7381 tggtgttagc cagtgcgccg tgctcttcca gtttacgtac cacgttagaa atggtggacg
     7441 ctttctggcc gatagcgaca tagatacatt tgataccgga atcgcgctgg ttgatgatgg
     7501 catcgatagc cagtgcggtt ttacctgtct gacggtcacc gatgatcaat tcacgctgac
     7561 cacgaccgat tgggatcatg gagtcaacgg ctttataacc ggtctgtacc ggctgatcta
     7621 cggactgacg ttcgataacg cccggagcga ttgcttctac agcagagaag ccgtcgtgat
     7681 ccagcggacc tttaccgtcg attggtgcac ccagagtgtt aaccacacgg cccagcaggc
     7741 cacggccaac cggaacttcc aggatacggc cagtacactt aactttcatg ccttcggcaa
     7801 ggtcagcgta cggacccata acaaccgcac ctacagagtc gcgctcgagg ttcagtgcga
     7861 tagcgtaacg gttacccggc agggagatca tttcaccctg catacaatcg gccaggccgt
     7921 gaatgcggat aacaccgtca cttacagaaa caatagtacc ttcgttgtga gcttcactca
     7981 caacattgaa ctgagcaatg cgctgcttga tcagttcgct gatttcggtg gaattcagtt
     8041 gcatgctcca gtccccttaa gactgcaaga cgtctgcaag gcgctcaaga cgaccgcgta
     8101 cgctgccatc aatgaccata tcacccgctc ggatgataac gcctgccatt acagacttat
     8161 cgattttgca attcagctta actttgcgtg acagacgttt ttccatcgca gcagaaattt
     8221 tcgcgagctg ttgttcactc agtgcggcag cggaaatgac gtctacctca gcggtagcct
     8281 cactcacggc acgaaggtga ataaactgct ccagaacatc cgggagcgcg ttaagacgac
     8341 cattttcagc cattacccga atcaggttct gaccgttttc gtccagttgc tcaccacaaa
     8401 ctgcgataaa cgactcggcg agcgtttctg gcgcaagcgc gccagagaga agctctgcca
     8461 tttgttcgtt tttggttacc tcggcggcaa acgccagcat gtcctgccag cgttctacac
     8521 tttggtgttc gacggcaaag tcaaaagctg ctttggcgta ggggcgagct accgtaataa
     8581 attcagacat cagcccctcc ctccttacag ttcagcgaca agtttatcca cgatgtcgct
     8641 gttagcagct tcatccacgg aacgttcgat gatcttctcg gcgccagcaa cagccaggat
     8701 agcaacttgc ttacgcagct cttcacgggc acgtttacgc tcggcttcaa tttctgcctg
     8761 cgcctgggcc acgattttag tacgttcctg ttctgcctca gctttcgctt cgtccagaat
     8821 ctgcgagcgg cgtttattcg cctgctcgat gattacctgg gcttccgctt tcgctttttt
     8881 cagctggtcg gtcgcgctgg cctttgcaag gtcaaggtcc ttatgtgctc gttctgcgga
     8941 agcaaggccg tcagcaattt ctttttgacg tttttcgatg gctgccatta atggcggcca
     9001 tacgtacttc atgcagaaca gaacgaacag gacaaacgcg atggcctggc cgaggattgt
     9061 tgcgttaaga ttcacagcac aatgcctcta tttagttaac gttctgatat tgctctttaa
     9121 ataaaagcaa cgcttactac gcgacagcga acatcacgta cagacccaga cctacagcga
     9181 tcatcgggat agcatccacc agacccataa cgataaagaa ctgagtacgc agcagaggaa
     9241 tcagatcagg ttgacgcgct gcgccttcca ggaatttacc cccgaggatg ccgataccga
     9301 tcgcagcacc gattgccgcc agacccatca tcacagcggc agccatgtac agcagatcca
     9361 tattcaggtt ttccatgaca gtctccagtt tgtttcagtt aaaacgtagt agtgttggta
     9421 aattaatgtt cttcagacgc catcgacaga tagacgatcg tcagaaccat gaagatgaag
     9481 gcttgcagcg taatgatcag gatgtggaaa atggcccacg gcacattcag gatccactgt
     9541 gaccaccacg gcaacagacc agcaatcaga atgaaaatca gctcaccggc atacatgtta
     9601 ccgaacagtc gcaaaccaag tgaaactggt ttggacagca ggcttacccc ttcaaggatt
     9661 aagttgacag gaatgaacgc ccagtgattg aacggctgca gcgtcaactc tttcgtgaag
     9721 ccgccgatgc ctttcatttt gatgctgtag aacagaatca ggataaatac gcccaatgcc
     9781 atagacagcg ttacgttcac gtccgcagac ggaaccacac gcagtgcagg cagacccagt
     9841 acatgttcag caatgtacgg cagcaggtcg ataggcagta aatccatcag gttcatcagg
     9901 aatacccaga cgaagatcgt cagggccagc ggagcaatca gcttgctttt gccatggtac
     9961 atgtctttca cgctaccatt aacaaagccg atcaccagct caatcgcggt ctgaaactta
    10021 cctggcacac cgctggtcgc ctttttggct acgctacgga ataaaaccag gaacaacaga
    10081 cccagcacca ccgagaagaa catggagtca atattgattg tccagaaggt ggctgggggg
    10141 tgttgtggat ccaccagcga gaatgtacgc aggtccagct gaaggttatt caggtggtgt
    10201 cctatgtaat cctgcggcgt catattttct gaagccatga tgccttttac cctttgttgt
    10261 taattacagc cggtgccagt atctgaacca ccagcaccaa aacccacgta acgatcagcg
    10321 gcaagaatac cgcctttaaa accgccaacg ccaccaccag taacaccaac atcgccagaa
    10381 ctttgaaagc ttcgccaaat gcgaatgtcc aggccacccg gcctttcgct ggtgtatgcg
    10441 cctggtgacg ccaggcaaat atcataaaca aaacgttagg cagaaagact gccaggcccc
    10501 cgcttattgc agagacgccc cagaaggggt ctttgaggct gaacagcaat ccacttgcta
    10561 tcaccaccag taactgaacg agcagaagct tccgagcaac gtttcgactc acgagcgaca
    10621 cagacatcac gtttttcact cctgctccct tcgaggtatg ccgcgtgtcg tataaaactt
    10681 tctttaaggc ttagagtcaa gcatcaaaaa gcggtcaaat tatacggtgc gcccccgtga
    10741 tttcaaacaa taagtagcca aaaggtgaat aaatgtttaa atatttttcc agtgcataca
    10801 attgcgactt ttctgctaac cctgttcgat catgaaaaac tgtaaataac gcgtaaacac
    10861 tggtgataaa gcgtgcttca gatcacatat tgcgcatgtt cgcgcacagc atatttattt
    10921 acttggcaaa tgatgccttt gcaagtttat gatat
//
LOCUS       DAZGVQ010000166        10687 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000166 DAZGVQ010000000
VERSION     DAZGVQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.167"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..424
                     /gene="tolA"
                     /locus_tag="ACHLAG_004262"
     CDS             <1..424
                     /gene="tolA"
                     /locus_tag="ACHLAG_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFP0657390.1"
                     /translation="CRAVPVLVLILASLTTGCTKDVSTNNYDASLHHSDKLIKSQNLS
                     LSERTPDASEEIRQYAIQVREAIEEQLKDAGKYSGKECSLRMYMAPNGLLLQVKRESG
                     DPDLCREAMNAVKNADIPSPPSSGVYKAFRNGVLDFKL"
     gene            891..1055
                     /locus_tag="ACHLAG_004263"
                     /pseudo
     CDS             891..1055
                     /locus_tag="ACHLAG_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483513.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
     gene            complement(1551..1636)
                     /locus_tag="ACHLAG_004264"
                     /pseudo
     CDS             complement(1551..1636)
                     /locus_tag="ACHLAG_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1893..3881
                     /locus_tag="ACHLAG_004265"
     CDS             1893..3881
                     /locus_tag="ACHLAG_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0657391.1"
                     /translation="MAFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSGDGETAISKTGEPEYYYVVALAGQSNGMSFGEGVPLPDSY
                     DRPDPRIKQLARRRTVTPRGVPCTYNDVIPADHCLHDVMDMSEKNHPRADLNKGQYGC
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSGVTTGNEGTFNASSGASPDSALWGADK
                     PLYQDLLSRTKVALAKNPGNILLAVIWMQGESDLASGSQSHNALFTGMVQKFRADIAD
                     MSGQCVGGSALSVPWVCGDTTHYWKEKYAASYETVYGGYKNKAAQNIHFVPFMVDGSG
                     VNVQTNNPAEDPDIEGIGYSGSKWRDSSVSWTSGDRASHFGSWARRGIISDRLATAIL
                     THAGKYPAFITGRSETVVAGDERENVTSGNFPSPVEDAALSQDAPQRRTVVEYNSAVE
                     KDSLGGNWAAQHWNARDGVAEFVNDESRQVLKIKQHEGKQGPWMLSAGVSAEDARALY
                     ENGGEIKLEFRLPDGTQVADDKYLFGVYLPVSGWTQEGNGSNMMASFFTQTKASNLDV
                     MYHGKTQPNTKIESFGAVNNNWHTLVFKYKGGGNHQVIPVLDGEEKTGFALKEGPVTS
                     AFTVDNITVTGISANQGKSYPVMFRSIMVSINQATSSS"
     gene            4028..4210
                     /locus_tag="ACHLAG_004266"
     CDS             4028..4210
                     /locus_tag="ACHLAG_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0657392.1"
                     /translation="MTFVHQLMLYFCTVVCVLYLLSGGYRVVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEDPLIP"
     gene            4248..4517
                     /locus_tag="ACHLAG_004267"
     CDS             4248..4517
                     /locus_tag="ACHLAG_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP0657393.1"
                     /translation="MSEIKSLVTAEAVKEVLRSEEVLSALKQKLRQNLEARLDAEVDA
                     ILDELLGAPTVPEPEGIADDSAVSDGVGSQPDGSEEPQPDGEMMM"
     gene            4592..4807
                     /locus_tag="ACHLAG_004268"
     CDS             4592..4807
                     /locus_tag="ACHLAG_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP0657394.1"
                     /translation="MYQMEKITTGLSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            4812..5345
                     /locus_tag="ACHLAG_004269"
     CDS             4812..5345
                     /locus_tag="ACHLAG_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0657395.1"
                     /translation="MSRKLRYGLSAAVLALIGAGASAPEIFDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLTKEKCDQVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     ISRRDQESALACWGIDR"
     gene            5863..6048
                     /locus_tag="ACHLAG_004270"
     CDS             5863..6048
                     /locus_tag="ACHLAG_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP0657396.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISTSENG"
     gene            6134..6358
                     /locus_tag="ACHLAG_004271"
     CDS             6134..6358
                     /locus_tag="ACHLAG_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657397.1"
                     /translation="MKKLRVTVEPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            6704..7030
                     /locus_tag="ACHLAG_004272"
     CDS             6704..7030
                     /locus_tag="ACHLAG_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFP0657398.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPRKGVKKTFI
                     FSPSAP"
     gene            complement(7162..7362)
                     /locus_tag="ACHLAG_004273"
     CDS             complement(7162..7362)
                     /locus_tag="ACHLAG_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0657399.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            7404..7769
                     /locus_tag="ACHLAG_004274"
     CDS             7404..7769
                     /locus_tag="ACHLAG_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP0657400.1"
                     /translation="MAQLRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSVREAASGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            8059..8622
                     /locus_tag="ACHLAG_004275"
     CDS             8059..8622
                     /locus_tag="ACHLAG_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP0657401.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDETNPEMPPPVVIS
                     PGIEYMEDGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            8619..10280
                     /locus_tag="ACHLAG_004276"
     CDS             8619..10280
                     /locus_tag="ACHLAG_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP0657402.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPSPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAAWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVL
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVETK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPGPKKKEYAVFFI"
     gene            10344..>10687
                     /locus_tag="ACHLAG_004277"
     CDS             10344..>10687
                     /locus_tag="ACHLAG_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657403.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLAKPVPDMPSQLAARLDEAWAAIK
                     TGLVRGLSVGFRP"
BASE COUNT         2596 a         2410 c         3116 g         2565 t
ORIGIN      
        1 ttgccgtgct gtaccggttc ttgttttgat attagccagt ctgacaactg gctgcacaaa
       61 agatgtatcg acaaataatt atgatgcttc gctgcatcac tcagataaat taataaagtc
      121 gcaaaatttg agcttatcgg aacgtacccc tgatgccagt gaagaaatcc ggcagtatgc
      181 cattcaggtc agggaagcca tagaagaaca gttaaaggat gcgggtaagt attcaggaaa
      241 agagtgttca ctgagaatgt atatggcccc gaatggtctt ctgctacagg ttaaaagaga
      301 aagcggtgat cccgatttat gtcgtgaagc gatgaatgca gtaaagaatg ctgatatacc
      361 atcaccaccg tcttcaggag tatataaagc atttagaaat ggcgtgctgg attttaaact
      421 ctgactgggg gagaagattt tctcctgtcc agatgatttt ggggtgctgg aaagatattg
      481 tgctgtgaag tgttaaattc ctcacaattc agtaagttga cagttgcctg tcagactggg
      541 catttgttaa aaaaatttcg catggtgaat ccccctgtgc ggaggggcga ctggtgaacg
      601 gtatgatctc tttgatgatc gtaagcgaga atacgcgggt ttagtgtcac cgggctgaac
      661 tcaccgggag gcacccggca ccatgctcat ggtgatacag aaatgcggct tcagcccctc
      721 tctggagggg ctttcttatg gacaaaaaag cccgcgctgg gagacgcggg cggcaaggaa
      781 taaacaataa aacgtgaagt aatatttcag ctggcgaata ataccccata gtaatcactc
      841 tgcgcaactg cgcggccttt ttcgaattgc gggctgtagt ctcccttctg ccgggtggcc
      901 tggttgcaga tttcttcatg gggtagggtt cggcagttaa agcagcgatg gcgctggggc
      961 gtcgtgctat aggcgttgag ctggataccg ggcgttttga acagactgcg caggcaatca
     1021 gggatatatt tatcggtagc tgtttgcaga agtaatctcc caaaattcca tttttggaat
     1081 gcagtggttg ttgctgtagt gtgggcaagt tctggaactg gcttcgtcca ttgcagatga
     1141 ttgttagctg gatatcagtt acctcatgga aatgttaaat gactcacaat tcggttgatg
     1201 gactattgtc tgattgatgg aagtcttgta aaaacgagcc agcatagtga atccccctgt
     1261 gcggaggggc gtctgacatg gtctccatag cgatggtcac aatacgcaga ttcagtagtc
     1321 aggctgaatc taccgggagg cacccggcac tatgctgctt atggtgtata cacagcgatc
     1381 ggcccctctc ctgaggggct ttttttgtgc gggaaaaggc tgtgttgtta aatgttaaat
     1441 ccctcacagt tcagatggtt gatgcttgcc tgtctgacgg ggagttgtta aaaaagttct
     1501 gcatggtgaa tccccctgag cggaggggca tatcagcgca ggtgtttcta ctctatcctt
     1561 tctgtgcggg ttcaggtgct gatactgaac tcaccgggag gcacccggca ccatgtagta
     1621 tacagagatt aggcatacat ccaggctcct cattgcagga gcctttttac atgcaaaaaa
     1681 accgctccag ggaagagcgg ttggcaagga ataaataaca aaacgtgaag taatttattc
     1741 agcgccctaa taatacctta cagtaatcac attgcgcaac tgtatgtaag ggcgtatttc
     1801 ttttttgcgg gctgttttgt gtgacttttg tgtttccgga ggtcagcccg agccttctct
     1861 gactcagaac attattccgg ccgggaggat tcatggcatt taaacactac gatgtggtca
     1921 gggcggcatc gccgtcagac cttgctgaac gactgacaca aaaactgaag gaggggtggc
     1981 agccatttgg cagccctgtc gccatcacgc cttataccct gatgcaggct attgcggcgg
     2041 aaggtgatgt caccacgccg gtgctggtta aaccatcggg tgacggggag acggctatca
     2101 gcaagaccgg tgagcctgaa tattattatg ttgtcgcgct ggcgggtcag tcaaatggta
     2161 tgtcttttgg agagggagta ccgttacctg attcttatga tcgccctgat ccgagaatta
     2221 agcagcttgc ccgtcgacgt accgttacgc cgcgaggcgt tccctgcacc tataacgatg
     2281 ttattccggc agatcattgt cttcatgatg tgatggatat gagtgaaaaa aatcaccccc
     2341 gggcagacct caataaaggt cagtatggtt gtgtgggtca gggactgcat attgccaaaa
     2401 aactgttacc gtttataccg gcgaatgcgg gcattcttct ggttccatgc tgtcgtggag
     2461 gctcgggagt gactacaggt aatgagggaa catttaacgc ctcttccggg gcaagccctg
     2521 attctgcatt gtggggagct gataagccgc tgtaccagga tctgttaagt cgcacgaaag
     2581 tcgcgctggc taaaaatccc ggaaatatcc tgctggcggt aatctggatg cagggggaga
     2641 gtgatctggc ttcaggaagc cagagtcata atgctctttt tactggcatg gtacagaaat
     2701 tccgtgctga catcgctgat atgtcagggc agtgcgtggg gggctcggca ctgagcgttc
     2761 cgtgggtttg tggcgatacg acgcattact ggaaagaaaa atatgctgcg tcttatgaaa
     2821 ctgtatacgg tggatataaa aataaggcag cgcagaatat tcactttgtc cctttcatgg
     2881 ttgatggaag cggggtaaat gtgcagacca ataatccggc agaagatcct gatattgaag
     2941 gtattggcta ctccggttcg aagtggcgag acagttctgt cagctggacg tcaggtgaca
     3001 gggcaagcca tttcggttca tgggcgcggc gtggaattat ttcagatcgt cttgcaaccg
     3061 caattctgac ccacgcaggc aaatacccgg cgtttatcac cgggcggtca gaaacggtag
     3121 ttgcaggaga tgaacgggaa aacgttacat ccggaaattt cccgtcacct gtggaggatg
     3181 ctgcgttatc acaggatgca cctcagagaa gaacggttgt tgagtataat tcagctgttg
     3241 agaaagacag cctcggtggt aactgggcag cccagcactg gaatgcccgg gacggtgtcg
     3301 ccgagtttgt taatgatgaa agcagacagg tgctgaagat taagcagcat gagggcaaac
     3361 agggaccgtg gatgctgtca gcaggagtat cagctgaaga tgcccgtgct ctgtatgaaa
     3421 atggcggtga aataaaactt gaattcaggt tgccggatgg tacccaggtt gcagatgaca
     3481 aatacctctt tggtgtttac ctgccagttt ccgggtggac tcaggaaggc aacggcagca
     3541 atatgatggc ctcctttttc actcagacaa aagccagtaa cctggatgtc atgtatcacg
     3601 gtaaaacaca gcctaataca aaaattgagt cattcggtgc ggttaataac aactggcaca
     3661 cgcttgtctt taaatataaa ggaggcggca atcaccaggt tattccggta cttgacggag
     3721 aagagaaaac aggttttgca ctgaaagaag gtccggtcac ttctgccttt acggtggata
     3781 acatcacagt gacaggtatc agcgcaaatc agggaaaatc ttatcctgtc atgttccgca
     3841 gtatcatggt aagcattaac caggcaacct cttcatcata aaacaaaaaa acgccagcgc
     3901 agaggagaac gggaggataa gtctgacgct ggcggaggag tatcctcatg gagaacgata
     3961 tgtaacacat atcgcctttc ttttattgaa aagtcatcgc cattgtcaac cataacggta
     4021 agaaactatg acatttgttc atcaactgat gctgtacttc tgtacggtgg tatgcgtgct
     4081 gtatcttctt tcgggtgggt acagagtcgt gcgcgatttc tggcgcaggc agattgataa
     4141 aagggccgct gagaaaatca gcgccagtca gtcagccgga agcaaacccg aagatccgct
     4201 cattccgtag tcactttctt gacaacacct ttcaacgaga aaatcctatg tcagaaataa
     4261 aatcgctggt cactgctgag gcagtgaagg aagtcctgcg ctctgaagaa gtcctgagcg
     4321 cactgaaaca aaaactccgc cagaatcttg aggcgcgtct tgatgcagaa gtggatgcca
     4381 ttctggatga actgctgggc gcaccgactg ttccggagcc ggaaggcatt gcggatgaca
     4441 gtgctgtttc agatggcgtc gggtctcagc ctgacggtag tgaagagcct cagcctgacg
     4501 gcgaaatgat gatgtaacca tgcgcagggg tgtcggtgtg agctgatgcc ccacttgttg
     4561 ttgtgagctt ccggattgcg ggagacgggg tatgtaccag atggaaaaaa tcacaacagg
     4621 tttgtcatac accacgtcag cggtgggaac gggctactgg ttcctgcagt tgctggacag
     4681 ggtttccccg tctcagtggg cggcaatagg cgtgctgggg agtctgctgt ttgggctgct
     4741 gacatatctg acgaacctgt atttcaaaat taaagaagac cggcgtaagg cggcgcgggg
     4801 agagtaagct gatgagcagg aaactccgct atggtttatc ggctgccgtt ctggcgctga
     4861 ttggtgcagg ggcgtctgcg cctgaaatct tcgaccagtt tctggatgaa aaagaaggca
     4921 accacaccac ggcataccgt gatggcgcgg gtatctggac catctgccgt ggagccaccc
     4981 gggtggatgg taagcctgtt attcctggca tgaagttgac gaaggaaaaa tgcgaccagg
     5041 ttaacgccat tgaacgtgat aaggcgctgg catgggtgga gaaaaacatc aaagtgccac
     5101 tgaccgaacc ccagaaagcg ggtattgcgt cattctgccc gtataacatt ggcccaggta
     5161 agtgtttccc gtcgacgttt tataaacgaa ttaatgcagg cgatcgcagg ggagcgtgtg
     5221 aggcgattcg ctggtggatt aaggacggtg gcagagactg ccgtattcgc tcaaataatt
     5281 gctacggtca gatatcccgt cgtgaccagg agagcgcgct ggcgtgctgg ggaatcgaca
     5341 gataagcaga atattttgct gaaaaatgac gttgaccaac gcggacggat aacacgaaat
     5401 cctgcgaact ggcaaaacct agtgaataaa agtaaaaacc ccgtttgttg gcagcaagcg
     5461 gggttttgtg tttcctgact ccggaaaagt caaaggagaa agtgtgttta attttagcaa
     5521 actgattcgg gagattcgag tgatggctga aaaattatcc acctggaagt tcatccttat
     5581 ctggctggtg tttgtgatta tggcctccgg ttatttcatc ggtcagatac gctggtggtg
     5641 aaatgaaccg cgttctgtgt gtggtcatta ttgccttgct ggtggcctgt ggtgcgctta
     5701 gtctggggct gaatcattac cgtgataacg ccatcaccta caaagcgcag cgcgataaaa
     5761 aagccagaga gctggagctg gcaaacgcaa ccattactga catgcagcag cgccagcgtg
     5821 atgttgctgc gcttgatgcc agatactcaa gggaattagc cgatgcgaga gctgaaaatg
     5881 aaactctgcg tgctgatgtt gccgctggtc gtaagcgcct gcggctcaac gccacctgcc
     5941 ccggtaccgt gcgtgaagcc accggcacct ccggcgtgga taatgcaacc ggcccccgac
     6001 tggcagacac cgctgaacgg gattatttca acctcagaga acggttgatg acaatgcaga
     6061 agcagctgga aggggcgcag gaatatatac gcactcagtg cactaagctg gctttttatt
     6121 atccggagga tacatgaaga aattacgggt aaccgtagaa ccttttcagg gaacaattcc
     6181 gttccgtatt ttgcagcgtg gtcgtgttct tgttgaaggt tcgttcagtg gtaaatgtac
     6241 gcaattacac tcccggacct ttcaggtgaa tgccacgaat gaagagctaa ccgttgagtg
     6301 tacgatgaat gccgctaaat gccgcatggt atccgctgca ttacagccag tgtgttgagc
     6361 gaccttatta tccatggcgc ggtattgtcg ccgtattccc gtattaacag agaccgcagc
     6421 ccgacaggga gactcctctg cgcgagtgtg cggggataat caaaaacgat acacaccggg
     6481 gtttaccgcg ttaacggagc gcggcgttgt cccctcatgg tcgctggtcc ggtgcgatgg
     6541 tggaagaaac cggacgatgt gttacctcgc aagctctgtt atgtcatgtg tctgatttgt
     6601 gatttaagtc ggataattgt cgttgccatt aagcagagga ttgatgaccg acagggtggc
     6661 attgttagaa taagacttat tcttatctgt gccgggaatg aaaatgaaaa gaaatcttcc
     6721 gttaattatt ttgttgtctt ctctggttat gggctgtacg caacataaaa cagatatgcc
     6781 ccgacagttg gttaaggcat taccacaata tccggcctat gcagcggcaa attatataaa
     6841 gggacgggtt gatgtgaagt ttgatattgg tgctgatggt actgtcaccc gaattgagtt
     6901 tatccgttca gagccgcacc atctgtttga tgagcaggtt gtaaaagcga tggcaaaatg
     6961 gcgatttgag aaggacaggc cgcgtaaagg cgtgaagaaa acgtttatct ttagtccttc
     7021 tgcaccctga ttatttcatc agaaattaat tatcactctg ttgttattct gtacatcccg
     7081 gcagggtaag tcttgttccg tcggatatga agatgaaata ttgttggagg acagtgggta
     7141 cctgctcctg taaccgaacg ttcatttctc gttatttgtc atgctggccg ggcgcagatg
     7201 cgttgcatct gttgccagcc ttctcctgca ggcttcaata acccacgctg aaaagttacc
     7261 ggacccttta tgctcaaggg cgatgttgat ctgttcaatc atgtgattgg ggaaacggat
     7321 attgcgggtt gtggttctgc gggtccggtt tttcgatgac atatttattt cctttactga
     7381 ttgccatatg acggggattt tacatggctc agcttcgtac actccagagc agaatcaaaa
     7441 cactgaatac ccgacgggtg aatattctga agggtgaaca gcgtcgtgtc agtggcagtg
     7501 cacgtgtttc cctcaagcgt catatctggc tcagggacgc cgggcagtgc tgtctctgtg
     7561 gtcgtgtggt tgacctctgt gacagtgaac tcgatcaccg aattgcactt cagttcggtg
     7621 gtggtaatga ggagacgaat ctctggacgc tctgtaccga atgccatcga caaaagtctg
     7681 ttcgtgaagc ggcgagtggt atgccggacc cgacactgcc ggaggtgtcc ggaggtagtg
     7741 gcagagcgga cgacatcatc ggactgtaac ccgacccggg ggggtatcat ccggcgtaaa
     7801 aaactatcgc tccggacacc gcgccccctc tcacgcagag aaaaaattcc cgtttcaggg
     7861 cagttaacat gttaactggc tgtccgggca tttttgcggt ttttatcttt attattcagt
     7921 ttgttgcgtg gaaaaaatgt taacaggctt tttcagcaaa tgttaaccag gcagcagtta
     7981 acatttgcgg catgagacgc cgggaaaaat gggctgaacc atacccggct gagtgcgttc
     8041 tggacccggg aggaggctgt gctgacaacg caaaaacgaa aatttgcgct ggcgctcatg
     8101 tccgggaaaa acaaaacagc gtcagccatt gccgccggtt attcggcgaa gacagccagg
     8161 gttaaaggct cgcagctggc aaaagatccg gaggtgcttg cgtttatagc ccgtaaacag
     8221 tgcgagacgg tggaggtgga tgaggttcct gtttaccggc agaaaaaatc agagcaggag
     8281 gataaacccc gtcgccgtga ggcggctgca ataccacagc cggacgaaac aaatccggag
     8341 atgccaccgc ccgtggtgat atctcctggt attgagtata tggaggacgg tcttcccgat
     8401 cctgtgaaag cgatggggcg tcttctggtg gagaacatta ataccgaccc caggctggcg
     8461 ctggatgcgg cttataagct ggcgcagttc acgcaccaca aaaaagggga tgccggtaaa
     8521 aaatcggcaa aaggtgacgc ggcgaaaaaa gcggctaacc gttttgcggt gccaccacca
     8581 ccccgcctgg tggtgaataa tgataatgag ggcaacggat gataccagtg tggagcacgg
     8641 cctgcccgga ctgggcagag cgtctgaaaa aggggctgtc gattattccg tctccgattt
     8701 acccggagca ggccgcacat gccctggcga tttttaaaca actgcggatt gtggatgcac
     8761 cgggcagccc gacgttcggg gagtcctgtg cagcgtgggt gtttgacctg gtggcggccc
     8821 tgtttggctc ctacgatgcg cagaccggtg tacgccatat aaaggaagtg ttcattctga
     8881 taccgaaaaa aaacagcaag tccacgctgg ccgcggggat catgatgacg gcgctgttac
     8941 tgaactggcg gcaggcggcg ggctacacca ttctggcccc gaccgtggag gtggcggcta
     9001 acgccttcaa ccctgccagg gatatggttc gacgggacga tgatctggat gacctctgtc
     9061 aggtgcagac acatatccgg accatcaccc atcgggtgac ggacaccacc cttaaggtgg
     9121 tggctgccga tccgaatacg gtgtccggta tcaagtccgt ggggacgctg attgatgaac
     9181 tgtggttatt tggcaagcag tacaaagcgg aggacatgtt acgtgaagcc ataggcggcc
     9241 ttgcctcccg cccggaaggg tttgtggtgt atacgaccac ccagtcgaat gagccgccag
     9301 ccggagtgtt cagacagaaa ctgcagtacg cccgggatgt ccgtgacggc aaaattcatg
     9361 atccgcactt tctgccggtg atatttgaac accctcctga aatggtggaa agcggggctc
     9421 acctgctgat ggaaaacctc gccatggtca atccgaatct cggttattcg gtggatgagg
     9481 cttttctgta ccgggagtac cgtaaagccc gggaggctgg tgaggaagca tttcgtggct
     9541 tcatgtcaaa acacgccaat gtggaaatcg gtcttgccct gcgttctgac cgctgggcgg
     9601 gcgcggattt ctgggagcag cagggcaggc gcgtcagcct ggacgatatc ctgcagcgcg
     9661 ctgatgtggt gacggtgggg attgacggcg ggggcctgga tgatctgctg ggaatgtacg
     9721 tgattggccg tgacagggaa acccgcgaat ggctgggctg gggccatgcc tgggcgcatg
     9781 aaaccgcggt gctcagacgg aagagtgagg catcccggtt tcaggatttt gtggcctgtg
     9841 gagacatgac gattgtccgt cgggtcgggg atgacacggc ggaagtggcg gagtatgtgc
     9901 gtcgtatcca tgaggctgag ttactggatc atatcggtat tgacccgtca ggtgtggggc
     9961 agattctgga ttcactggcg gaagccggga tccccgacgg aattgttgtg gggataagcc
    10021 agggctggaa gctgggcggg gccatcaaaa ccaccgagcg caaactggct gagggagtgc
    10081 tggtgcatgg tggtcagcca ctgatggcct ggtgcgttgg caatgcccgg gtggagacta
    10141 aaggtaacgc catccttatt accaaacagg ccagcggacg ggggaaaatt gacccgctga
    10201 tggcgctgtt caatgcggta tccctgatgt ccctgaatcc ggggccgaaa aagaaagaat
    10261 atgcggtttt tttcatataa ccctgttcac cctgtaacca tcatggaccg ctgcggcggt
    10321 ttttttattt tcaggaggct gatgtgactc ttaaacgggc ctgctccctg ctgacggtga
    10381 aatccttcag tgaggatgag cgggtgatca ccgggattgc gtcaacgcct tctccggatc
    10441 gggatggtga catcctggag ccggagggcg cggagtttgg cagtgcgatc ccgtttctct
    10501 ggcagcatga ccattcccgc ccggtggggc agtgtacggt gcgccgggtc agcgaagggc
    10561 tggaaatcac ggcaacactg gcgaagcccg tgccggatat gccatcgcaa ctggctgccc
    10621 ggctggatga ggcctgggcg gccattaaga ccgggctggt cagggggctg tccgtgggct
    10681 tccgtcc
//
LOCUS       DAZGVQ010000167        10672 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000167 DAZGVQ010000000
VERSION     DAZGVQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.168"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            68..799
                     /gene="artJ"
                     /locus_tag="ACHLAG_004278"
     CDS             68..799
                     /gene="artJ"
                     /locus_tag="ACHLAG_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFP0657404.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(974..2101)
                     /gene="rlmC"
                     /locus_tag="ACHLAG_004279"
     CDS             complement(974..2101)
                     /gene="rlmC"
                     /locus_tag="ACHLAG_004279"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFP0657405.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(2142..2630)
                     /locus_tag="ACHLAG_004280"
                     /pseudo
     CDS             complement(2142..2630)
                     /locus_tag="ACHLAG_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
     gene            complement(2690..3535)
                     /gene="potI"
                     /locus_tag="ACHLAG_004281"
     CDS             complement(2690..3535)
                     /gene="potI"
                     /locus_tag="ACHLAG_004281"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFP0657406.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            complement(3532..4485)
                     /gene="potH"
                     /locus_tag="ACHLAG_004282"
     CDS             complement(3532..4485)
                     /gene="potH"
                     /locus_tag="ACHLAG_004282"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFP0657407.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDTYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            complement(4495..5628)
                     /gene="potG"
                     /locus_tag="ACHLAG_004283"
     CDS             complement(4495..5628)
                     /gene="potG"
                     /locus_tag="ACHLAG_004283"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFP0657408.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(5723..6835)
                     /gene="potF"
                     /locus_tag="ACHLAG_004284"
     CDS             complement(5723..6835)
                     /gene="potF"
                     /locus_tag="ACHLAG_004284"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFP0657409.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     NLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(7186..7662)
                     /locus_tag="ACHLAG_004285"
     CDS             complement(7186..7662)
                     /locus_tag="ACHLAG_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFP0657410.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(7750..8652)
                     /gene="rimK"
                     /locus_tag="ACHLAG_004286"
     CDS             complement(7750..8652)
                     /gene="rimK"
                     /locus_tag="ACHLAG_004286"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFP0657411.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(8713..9435)
                     /gene="nfsA"
                     /locus_tag="ACHLAG_004287"
     CDS             complement(8713..9435)
                     /gene="nfsA"
                     /locus_tag="ACHLAG_004287"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFP0657412.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISDAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     AENPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(9419..9706)
                     /locus_tag="ACHLAG_004288"
     CDS             complement(9419..9706)
                     /locus_tag="ACHLAG_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFP0657413.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9866..10123
                     /gene="grxA"
                     /locus_tag="ACHLAG_004289"
     CDS             9866..10123
                     /gene="grxA"
                     /locus_tag="ACHLAG_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFP0657414.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(10153..10530)
                     /locus_tag="ACHLAG_004290"
     CDS             complement(10153..10530)
                     /locus_tag="ACHLAG_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFP0657415.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKKQ"
BASE COUNT         2794 a         2884 c         2582 g         2412 t
ORIGIN      
        1 atggcattgt tatcccatgc cgcagacacg gccaaaaatc ataagattga cagacgggag
       61 ttccatcatg aaaaagttag ttcttgccgc tttacttgct tcctttactt tcggtgcttc
      121 tgccgcagag aaaatcaatt ttggcgtttc agccacctat ccaccctttg aatctatagg
      181 tgctaataat gagattgtcg gctttgatat cgatctggca aaagccttgt gcaaacaaat
      241 gcaggcagaa tgtactttta ctaatcacgc gttcgacagc ctgatcccgt cactgaaatt
      301 cagaaaatat gacgccgtaa tctccggtat ggatatcacg cccgagcgta gcaagcaggt
      361 atcgtttacc acgccctact atgaaaactc agccgtcgtg attgccaaaa aagataccta
      421 caaaacgttt gccgatctga aaggcaaacg tattgggatg gaaaacggca ccacgcacca
      481 gaaatatatt caggatcagc accccgaagt gaaaactgtc tcctatgaca gttatcagaa
      541 tgcctttatc gatctgaaaa atggtcgtat tgatggggta tttggtgaca cagcggtggt
      601 aaacgaatgg ctgaaaacca atccgcaact gggcgttgct actgaaaaag tgaccgatcc
      661 gcaatacttc ggcaccggtc tgggcatcgc tgtacgtccg gataacaaag ctctgctgga
      721 aaaactgaat aacgcgctgg cagcaattaa agctgacggt acatatcaga aaatcaatga
      781 ccagtggttt ccgcagtaat tgagactgat gacaaaagta agattgcctg atgcgctgcg
      841 cttatccggc ccacatcaac tctgcaatgt attgatttag catgattttg taggccggat
      901 aaggcgtaca cgccgcatcc ggcatgaaca aagcgcacct gatcagcaat ctgaagccgc
      961 acctgcggct tttttattgc ttcaccagca gcgtcagcac ttcatagtgc gcggtgtgcg
     1021 ggaacatatc aaaaagctgt acccgttcga tgcggtaacc tggcagttcg cggatatctt
     1081 tcgccatggt ttgggcgtta cagctggagt agataataaa acgcggtgcc attgttgaga
     1141 gataatcaca tagcggttta ccaatgccgc ggcgcggcgg attaaccagc accagctccg
     1201 gcacctcccc ctgagcggtg gcaaactgag tggagtccag cgcctgaaat tgcaaacgcg
     1261 ttaagcccag ttcagcggct gactgctttg cacaggcaat ggcctctgat gcaatttcga
     1321 tcccggttaa ctgcatgtca ggcgtcgcgc agtgtaaacc aaagccaccc acaccgcaga
     1381 acagatccca catatgttta actggcagct gccgtaccca gtcgcgcgcg gtagcgtaca
     1441 gctggctggc gaccgccgga ttagtctgga agaaactttg cggacggatc cacagcggta
     1501 cgtcattaaa acgctctgcc agcgcctgtt gttcagtaag gtagatctcc gtctcccctt
     1561 ccataatcgc catatgtacc ggctgaatat tgacggtaat aactttcagt tgcggtagtt
     1621 gttcctgtaa ccacggcagc gccttacgca gttgtgccag tttggtttca gaacgcagta
     1681 caaagcgcag catcatgccg ccatcgctct ggctttcagt cagcagaatg tatttcagtt
     1741 cgccacgctt acgcgccacg ttgtagggtg tcagccccgc acgggcgata aacggtttta
     1801 gcgccgcaaa aacgggcgca aatgaggcag gataaagcgg gcagtcacaa aggtcttctg
     1861 gtgtgccgtc gcgatgcagc ataccgagca gtggtttttc aacgctacca ctcaccacca
     1921 ttttggcttt attacgaaac ccttgttccg ggcctgacac cggcgcgcac cattcctcaa
     1981 ccggaaaatc ggcgagcaga tttttaagat cggcggtttt agcggagagt tgctctggaa
     2041 tcggctgcgt tatccactga caggaacgac agcgacccgc gtcgtaaagt gcgcactgca
     2101 tacattgacc ttcacatcat caggggcgac gattatacac attattgcaa ctggaagaac
     2161 cgccgactgg tcgagggaac gaacagcagc atcaggatga gcatatccgg cagcttctgc
     2221 agcatcaggc tatggaagat ttcacgtttt gattcaccgg gaatgctgaa cagctccgga
     2281 taaccgtacc ccagcgaagc cgcccacaag taacttgcgg cggtgatttg tgtcagcaga
     2341 tatagccagc gcgcccagcg acgccctttc accagtgaaa acgcacacca gatctcaatg
     2401 aaaaccagca ccagactgct taaaaagacc agcgttaaac tctaggtttg tacgctgcga
     2461 tgaatgaact cgccaatacc gcgcacgccc agcgtattga aaatcatcag cacgtcgagg
     2521 ccacggatca taataatggc gagcgccgcc acctgcacca gcgcaggcac attcaggcga
     2581 gcatgagatg aagatgtttt cttaaaaaat cccaacgttt cgtcttccat gaaaacaatg
     2641 ccgcggcatg cgcggcatta tgtagccagg ttggcaaatt ttagtgtctt cagccatgtc
     2701 ttgcacgctg gatatcgcgt atccgctgtt tttctgcgcg agccatcaga taccaggcga
     2761 taaatccgac aattccgacc gcaccgagaa ttaacgttgc aagggcgttg atttccggat
     2821 tcacccccat ccgcacgctg gaaaagacca gcatcggtaa cgtggtggct cccggcccgg
     2881 aaacaaagct ggcgatcacc agatcatcaa gcgacaaagt aaaagccagt aaccagccag
     2941 aaatgatcgc gggcatgatc atcggtagcg taatgacgaa aaacactttc agcggcgtcg
     3001 caccgagatc catcgctgct tcttctatcg agcgatccag ttcccgcaat cttgaagaaa
     3061 taacgaccgc cacataagcc gtacaaaaag tgacatgcgc cagccagatg gtgagcatac
     3121 cgcggtccgc aggccagcca atggcatgag caagcgcgac gaataataac aacagcgaca
     3181 agcccgtgat gacatctggc atcaccagcg gcgcggtgat cataaaggca aagccatttg
     3241 atccgcgaaa cctgccaaag cgcaccagca ccaccgccgc aatagtcccg aggatcgccg
     3301 ccgccgttgc cgcacaggca gcaattgtca ggcttaaacc aaccgcactc atcatcgcgt
     3361 catcgcgcaa taactcacca taccagcgcg ttgaccagcc ggcccacacc gtcaccagct
     3421 tcgagctgtt aaacgaatag ataaccagca tcagcattgg cgcgtagaga aaggtaaagc
     3481 ccagcagcaa aatcacaatc cgccagggcg aacgaactac cggtaaatta ttcatccgtg
     3541 ttctcccacg cttttttgct ggtgtttgtg gaaccacatt atcggcacaa ttagcagcag
     3601 caacatgatg atcgctaccg ccgaggccac cggccagtcg cggttattga aaaactcttg
     3661 ccatagcacg cgcccgatca tgatgctgtc cgggccaccg agcagttccg ggatcacaaa
     3721 ctcgcccaca gccgggataa acaccagcat cgatccggca ataatcccac ctttagtcag
     3781 cggcacgatc acagtaaaga acgttttcag aggtcgtgca ccgagatcca gcgctgcttc
     3841 caccagcgaa taatcaatac gaatcaacgc ggtataaatc ggcagtacca taaacggcac
     3901 gtaagcgtaa acaatgccga tataaacggc cagattggta tgcagaatgg tcagcggttg
     3961 atcgataacc cccagccaca gcagaaaatt attcagcaca ccgttgtttt ttaatattcc
     4021 catccaggca taaacgcgga tcagaaacga ggtccacgac ggcaggatca ccagtagtaa
     4081 taagatatta cgggtcgaag gcttactgtg cgccaccgcc cacgccagcg gatagccgat
     4141 cagtaaacag caaaatgtcg aaatcgcagc cacctgtagc gactggagat aagtatcgaa
     4201 atagagcgga tcgtcggtca gttgcagaaa attaccgaga ttaagagtga tggacagttg
     4261 cccgtcagcc cactccatca gttcggtata aggtggaata gcgcgcgcca tctccgccag
     4321 gcttatttta aagacaatca gaaatggcag cagaaacagc aagatcaacc agatatatgg
     4381 caacgcaatg accagtttgc gcccatgctt catttgcagc tgcgacaacc acagcttaaa
     4441 tccgcccggc ggtttcgact gggcagcagg ttcaagtgta ttcatctttg ctccttaaac
     4501 cgtcagcacc acacagctgt ccacttccca gcacaaacgc acttcgtcgc cccaggtcgg
     4561 taacccttta cgatgacgat gggcgttttg tagctgggcg ctaatcatct gcccactttt
     4621 cagacgaacg tgatacaccg aaagatcgcc gagataggca atgtgtatca cctcccccac
     4681 cgcgaagtta caaccattgg cgggcggctc ttcgcaaagc atgatttttt ccgggcgcag
     4741 cgctacgtgt accggcacgt tatcgaccac tgaagcatcc gcatcgactt tcagtggatg
     4801 caccagcccc ggcgaatcaa gcaccaggcc atcttcctga cgctctttga gtacaccttc
     4861 aaagacatta accgaaccga taaattcagc gctgtagcgg gtagttggat gctcgtagat
     4921 ctcttccggc tcgccaatct ggacaaattt cccgcgattc ataatggcga tacgccccgc
     4981 catggtcatc gcctcttcct gatcgtgggt gaccatcaca caagtcacac cgacgcgctc
     5041 cagaatatcc accacttcaa gctgcatccg gtcacgcagc tttttatcca gcgcgcccat
     5101 cggctcatcg agcagtaata gtttcgggcg tttggcaagg cttcgggcca gggccacacg
     5161 ctgtcgctga ccaccggaaa gctgatgcgg tttgcgtttg gcgaactcct gcatgtgcac
     5221 cagcccaagc atctcgttga cccgggtggc aatttccgct ttcggtagtt tgtcctgttt
     5281 caggccaaag gcgatgttct gctccacagt catatgggga aacaatgcgt aagactgaaa
     5341 catcatattg atggggcgta gataaggtgg cacctgtgac aaatcgacgc catcaagcat
     5401 aatctgtccg gcagagggtt gttcaaaacc tgctagcata cgcagcagcg tggatttacc
     5461 acagccggaa gcgcccagca gcgcgaagat ttcacctttg tagatagtca ggctgacatc
     5521 atccaccgca tgttgaccat cgtaggattt ggtgaggttg cggatttcta atagcggcgt
     5581 cagcgcctta cgggttttcg cctgcgggcg agggatagcg tcattcacgg ctgctctccg
     5641 gcattgaatg tgtgcaagcg caccatgatg gtgcgaattg ccggcgggtg tggtgcgccc
     5701 gcccggcatc taaggctgcg gattattttc cgctcttcac tttggtccac gcgcgggtgc
     5761 gcacacggtc gattttcgga tcctgcactt tcagagtgaa cagcttcgca cggacatccg
     5821 caggcggata aatacccggg ttatcacgga cttccgcact caccagcggc gtggctgctt
     5881 tattggcgtt ggcatagaac acatggtcgg aaatatgcgc tactacatcc gggcgcagca
     5941 ggtaattcag gaactgatag gcttcgtctt tgtttttggc atccgcaggc atggcgaaga
     6001 catcaaagaa cgccatcgcc ccttcttttg gaatcgagaa cgagacatta acgccattct
     6061 tcgcttcctt cgcgcggttt gacgcctgcc agacatcgcc cgcccagcca atagcgacgc
     6121 agatatcgcc gtttgccagg tcgttaatgt attgagatga atggaaataa cgaatgttcg
     6181 ggcgcagctt taacagcaga tcggtagccg gtccggtgta atcatccgct ttagtgctgt
     6241 tgggatcttt gccgagataa ttcagcacgg tagcaaaaac ttcttctggc gcatccagga
     6301 aagagacacc gcagcttttc agtttttcca gattttcagg cttgaggatc aagttccagc
     6361 tatcgacggg cgcgttttcg cccagcaccg ctttaacttt atcaacgtta tagccaatcc
     6421 cggtcgtcgc ccacatatag ggcatagcaa atttattgtc gggatcgtgt ttggcgacca
     6481 acttcagcag ttccgggtcg agattcttcc actccggcaa tttgctttta tccagcggct
     6541 ggaaaactcc cgctgtcagc tggcgttcaa gaaagcttgc tgacggcacc accaggtcaa
     6601 agccggtgct cccggccatt aatttgcctt ccagtacttc gttagagtcg aaaacatcgt
     6661 agacgacttt aataccggtt tctttttcaa aattggccac cgtgtccggg gcgatataat
     6721 cagaccagtt ataaatgtgg agtgtttttt gttcagccgc gagcgtgccg acagagacgg
     6781 ccatcagagc acccgcaacc agacccgata gccatttttt atttaaggcg gtcatatctc
     6841 tttccttctg aaagttcgtt aacaaacgaa caaaagttgt cacactgaaa gatatgcaat
     6901 aaacgtgcat attttttatc ggcgcggtgt aagaaaagag aactctctcc cggccagcac
     6961 tgctcgctat ataaaagcat cgcaagaata actttagcca gggtttgaga ctatagctcg
     7021 gattaaaaaa cggtatttaa caaattttta tgcgtatttg ctctatgtga taaagccaaa
     7081 tcgacacaag agaagtgaaa taatctttaa gaaaaggtta aaaacagcag ataattttgc
     7141 agcgcgcagc cactgcacca gcgactgcgt gctcgaaagg agttatcagt ggaggaaata
     7201 gttttcggta acgttgtttt gcccttcatc atcagtcggt aacagcagtt gatgggcatt
     7261 agcttcaaga atgaccatcg aaatctgctc ttcgctctga cgcacgaacc atgcaaactg
     7321 ctcataggtt acgccctgca taacggataa agactggcaa accaccagct ttggcagatt
     7381 atcatcctgc atatcaagaa atgctttcac ggtcagcgaa ctggcattga tggcagataa
     7441 atccgccgcc agcggcaata cggctgaagg tcggacttcc gccatggcag aaaacaggat
     7501 cgtgttatcg atcagatcga ttttggcatc aaagacaccg tcgaaattct gcatatgggg
     7561 cagatgcaga gcctgacagt tatcacattc aaaaaaactc atccccaggt catcgagcca
     7621 ttgacgcagc gtatccagac caggaacgac cagcgatgtc ataattttgt acaacctctt
     7681 tcgataaaaa gaccggcaca gcttacgcaa aaagcgcagg caaaaccatg atcagtaatg
     7741 tgattgcgac taaccacccg ttttcaggca atattctgtc gtagcgtggc gttcgatcca
     7801 gcggatcatt ttacccgcga tgtcgatacc ggtggttttt tctattcctt ccagccccgg
     7861 cgacgcattc acctccatca ccaacggccc gcgattagca cgcagaatat ccacaccagc
     7921 aacgtccagc gccatcgttc gcgcggcttt tatcgcgatt tcacgctcct gtggtgtgat
     7981 actggcgacg cttgccgcgc cgccacgatg caaattggaa cgaaaatcgc cctctttcgc
     8041 ccgccgttca atcgcagcga ccacttcatc gccaacaacc agacagcgga tatcgcaccc
     8101 ttgcgcctct ttgatatatt cctgcaccag aatatgcgcg ttcagaccgc ggaaagcgtc
     8161 aatcacgctt tccgccgcct gacgcgtctc cgccagcacg acgccaattc cctgcgtgcc
     8221 ttcaaccagc ttgaccacca gcggcgcacc accgaccatg tcgattaaat cgctggtatc
     8281 atccggcgaa tgcgcaatgc ccgtgacagg caggtcgatg ccctgacgcg ccagcagttg
     8341 catggaacgc aatttgtcac gcgcccgggc aatggcgacc gactcattga gcggatagct
     8401 ccccagcatc tcgaactggc gcagtgccgc cgtcccataa aaggtaatgg cggtgccaat
     8461 acgcgggatc actgcgtcaa aatggggtaa cttgcggcct ttgtagtgaa tagaagacgc
     8521 cgcaggattt atgttcatgt agcaagaaag cggatcaaga atttcaacca ggtgaccgcg
     8581 ctgtatagcg gcttcacgca gccgcttaca cgaatagagc gttccatccc gggacaatat
     8641 ggcaattttc accctgcacc tctctgtcag acctggtcaa aagcccgcgt atcatacatc
     8701 gacgtggcgg ttttagcgcg tcgcccaacc ctgtttgtgc aaataatcca gaataaatgg
     8761 gcggctttct ttaatgattg ttcggcggat atgatcgctc caggtatccc ggcgattatt
     8821 gctgccacgg gtgaggtaat attccgccag ttgctcgtca tactgcgcca gtgcgccttt
     8881 atccagcggt tgatagctgt tttcatgcac caaaatggag gccggtaaac gcggcttaag
     8941 atccggattt tccgcaggcc agccaaggca cagcccaaac agcggcagaa catgctgcgg
     9001 taatttaagc agtttcgtca ccgcttcaat attattgcgc aggccgccga tatatacccc
     9061 gcccaatccc agcgattccg ctgcgattaa tgcattctgc gccatcattg ccgtatcaac
     9121 gacaccgagc aacagttgtt ccgccaggcc gagctgagca tccggacaga tctgtaaatg
     9181 gcggttaaag tcggcacaga acacccagaa ctccgccgct tgcgctacgt gtttttgccc
     9241 gccggtcagc gtcaccagtt cttcacgtaa cgctttgtcg gtaatgcgaa taatgctact
     9301 gcactgcaaa aaactggaac tggacgtcgc acgggcgctg ttaataatcg cctcacactg
     9361 cgcgtcggaa atgggttcat cagtgaaatg gcgaatggag cgatggccac aaataagttc
     9421 aatggttggc gtcattatct ttttctcttt ctgaacgtga atattgcggt ggacggttca
     9481 tcagctgtgg ggcaagacgt tttgccacct gaagaataac caccaccgca gcgggaagca
     9541 tgagcaaaac accgagaaaa atcatcagaa tctgcacttc tggccgagaa aatggctcag
     9601 gcagcgacag ggagtcgctt accgacagca gcgccaccgc cagtagcatc attccgataa
     9661 attccagtat caacacgcct ttaggcaatt taccgatcgc gcgcatacgc ttccctctgc
     9721 aaagtgagcc ttcagtctaa aacttttcac tgtattgtgt ttaacagtta tagcttttag
     9781 caattaatgc aacaggttaa acctactttc agcgaataca ttttagcgtg atcattacag
     9841 gcataaatct atgaggagag aaataatgca aaccgttatt tttggtcgtt cgggttgccc
     9901 ttactgtgtg cgtgcaaaag atctggctga gaaattgagc aatgaacgcg atgattttca
     9961 gtatcaatat gtagatattc gtgcggaagg gatcactaaa gaagatctac aacaaaaggc
    10021 aggtaaaccc gtagaaaccg tgccgcagat ttttgtcgat cagcaacata tcggcggcta
    10081 taccgatttt gctgcatggg tgaaagaaaa tctggacgcc tgatcgtctg acaagccctc
    10141 gcgttgaggg ctttactgtt tttttctgtg ctgtggttta aacaaactac tgataaataa
    10201 gaaacacagt gcccccagcg cacaccagaa caccgcgctt agtaaccatg ccagctcttg
    10261 ccagaatgag cgcgtcggtg aaaaaaacag ccgcataatg agcatcgaac agggtgccgc
    10321 cagcattgcg ccaaacagag gtttcaggac ttctctacgc tgtgaaaaga agctggccac
    10381 tgctccagga agaatgaaaa atagcaagcc gatttcagga tgcccggcag cccgaaaagc
    10441 gcctttcatg tgcgtcgcca gaaaaaggca caccacaatg aagaggacaa aacagcagat
    10501 tgcccccgcc caacgttgtt tatgtttcac tcgttcctcc tgacactgcg tctatcgaac
    10561 acatttttcg ccagtgtggc gttcagtaag ataaagccgc ttcgcattcc atgctaatat
    10621 aggccaacgc aattcatata gccgttgata cctaatgtga ttacactagt aa
//
LOCUS       DAZGVQ010000168        10426 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000168 DAZGVQ010000000
VERSION     DAZGVQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.169"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            127..702
                     /gene="ssuE"
                     /locus_tag="ACHLAG_004291"
     CDS             127..702
                     /gene="ssuE"
                     /locus_tag="ACHLAG_004291"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP0657416.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            695..1654
                     /gene="ssuA"
                     /locus_tag="ACHLAG_004292"
     CDS             695..1654
                     /gene="ssuA"
                     /locus_tag="ACHLAG_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFP0657417.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1651..2796
                     /gene="ssuD"
                     /locus_tag="ACHLAG_004293"
     CDS             1651..2796
                     /gene="ssuD"
                     /locus_tag="ACHLAG_004293"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFP0657418.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGAAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2807..3598
                     /gene="ssuC"
                     /locus_tag="ACHLAG_004294"
     CDS             2807..3598
                     /gene="ssuC"
                     /locus_tag="ACHLAG_004294"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFP0657419.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3595..4362
                     /gene="ssuB"
                     /locus_tag="ACHLAG_004295"
     CDS             3595..4362
                     /gene="ssuB"
                     /locus_tag="ACHLAG_004295"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP0657420.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4569..7181)
                     /gene="pepN"
                     /locus_tag="ACHLAG_004296"
     CDS             complement(4569..7181)
                     /gene="pepN"
                     /locus_tag="ACHLAG_004296"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFP0657421.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDELWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDISRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7447..8649
                     /gene="pncB"
                     /locus_tag="ACHLAG_004297"
     CDS             7447..8649
                     /gene="pncB"
                     /locus_tag="ACHLAG_004297"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFP0657422.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8818..10218
                     /gene="asnS"
                     /locus_tag="ACHLAG_004298"
     CDS             8818..10218
                     /gene="asnS"
                     /locus_tag="ACHLAG_004298"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFP0657423.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2363 a         2699 c         2794 g         2570 t
ORIGIN      
        1 tctttagttt attttcagaa aaagatacac aaaatttata tttggcattt tcttgtctct
       61 ccgacagact gaccgaattg ttatcaatgt taacaaaaaa agaacaattg gttataagga
      121 gagagtatgc gtgtcatcac cctggcgggt agtcctcgct ttccttctcg ctccagctcc
      181 ttgctggaat atgcgcggga aaaactaaat ggcctcgatg tagaggttta tcactggaat
      241 ctgcaaaact tcgccccgga agatctgctt tatgctcgtt tcgatagtcc ggcactcaag
      301 accttcaccg aacagctgca acaggctgat ggactgatag tcgctacacc tgtgtataaa
      361 gccgcctatt ccggcgcgct gaaaaccctg ctcgacctgc tgccagagcg cgctttgcaa
      421 ggcaaagtgg tgctaccgct ggcgacgggc ggtaccgtgg cccatctgct ggcggtcgat
      481 tatgccctta aaccagtttt aagcgcactg aaagctcagg agatcctgca cggcgtgttt
      541 gccgatgact cacaagtaat tgattaccat cacagacccc agttcacgcc aaatctgcaa
      601 acccgtcttg ataccgcgct agaaactttc tggcaggcat tgcaccgccg cgatgttcag
      661 gttcctgacc ttctgtctct gcgaggtaat gcccatgcgt aacatcatta aactggcgct
      721 ggtgggattg cttagcgtct ctacgattgc ggttgctgca gaatcctcgc ctgaagcgtt
      781 acgtataggc tatcagaaag gcagtattgg tatggtactg gcaaaaagcc accagttact
      841 ggaaaaacgc tatccgcaat caaaaatctc ctgggtggag ttccccgcgg gtccgcaaat
      901 gttggaagcg ttaaacgttg gcagtattga tctcggcagt accggggata ttccgccaat
      961 ctttgcccag gctgccgggg ctgatttggt atatgtgggc gtcgagccac cgaagcccaa
     1021 agccgaagtg attctggtgg cagaaaacag cccgatcaaa accgtagccg atcttaaagg
     1081 tcacaaagtt gcctttcaga aaggttccag ttcacacaac cttttactgc gtgcactgcg
     1141 tcaggccgga cttaagttta ccgatatcca acccacttac ctgacgcccg ctgatgcccg
     1201 cgccgcgttc cagcaaggta acgttgacgc ctgggctatc tgggatccct actactctgc
     1261 tgcattatta cagggcggcg tgcgggtgct gaaagacggc accgatctca atcaaactgg
     1321 atcgttttat ctggcagctc gtccctatgc agaaaaaaac ggcgctttta ttcagggcgt
     1381 actcgcaacc tttagtgagg ccgatgcgtt aacccgcagc cagcgcgagc aaagcatcgc
     1441 tttactggca aaaacgatgg gcttaccggc accggtgatt gcctcttact tagatcatcg
     1501 ccctcctacc accatcaaac cggttaacgc cgaggttgcc gccttacaac agcaaacggc
     1561 agatctgttt tatgaaaatc gtctggtgcc gaaaaaagtc gatattcgcc agcgcatctg
     1621 gcagcccact caactggaag gaaaacaatt atgagtctga atatgttctg gtttttaccg
     1681 acccatggtg acgggcatta tcttggaacg gaagaaggtt cacgcccggt tgatcacggc
     1741 tatctgcaac aaattgcgca agcggcggat cgtctgggct ataccggtgt gctgatccca
     1801 acggggcgat cgtgtgaaga tgcgtggctg gtggcggcgt cgatgatccc ggtgacgcag
     1861 cggctgaagt ttcttgtcgc cctgcgcccc agcgtaacct cacctaccgt tgccgctcgc
     1921 caggccgcca cgcttgaccg tctctcaaat ggtcgtgcgt tgtttaatct ggtcacaggc
     1981 agcgatccac aagagctggc aggcgacggc gtgttccttg atcatagcga gcgctacgaa
     2041 gcctcggcgg aatttaccca ggtctggcgg cgcttattgc tgggggaaac cgtcaatttc
     2101 aacggtaaac atattcatgt gcgcggagca aaactgctct tcccgccgat tcaacagcct
     2161 tatcctccgc tttactttgg cggatcgtca gacgtcgccc aggagctggc ggcagaacaa
     2221 gttgatctct acctcacctg gggcgaaccg ccagaactag taaaagagaa gatcgaacaa
     2281 gtgcgggcga aagctgccgc gcatggacgc aaaattcgtt tcggtattcg tctgcatgtg
     2341 attgttcgtg aaactaacga cgaagcgtgg caggccgccg agcggttaat ctcgcatctt
     2401 gatgatgaaa ctatcgccaa agcacaggcc gcattcgccc ggacggattc cgtaggacaa
     2461 cagcgaatgg cggcgttaca taacggcaag cgcgacaatc tggagatcag ccccaattta
     2521 tgggcgggcg ttggcttagt gcgcggcgct gccggaacgg cgctggtggg cgatggtcct
     2581 acggtcgctg cgcgaatcaa cgaatacgcc gcgcttggca tcgacagctt tgtgctttcg
     2641 ggctatccgc atctggaaga agcgtatcgg gtcggcgagt tgctgttccc acatctggat
     2701 gtcgccattc cggaaattcc ccagccgcag ccgctgaatc cgcaaggcga agcggtggcg
     2761 aatgatttta tcccccgtaa agtcgcgcaa agctaaggaa gaaataatgg caacgccagt
     2821 gaagaagtgg ttattgcgcg ttgccccctg gtttttaccg gtgggcatcg tggcggtgtg
     2881 gcaactggcc tcctcagttg gctggctgtc gacgcgtatt ttgccctcac cggaaggggt
     2941 ggtgacggcg ttctggacgc tctccgccag cggcgaactg tggcagcatc tggcgatcag
     3001 ttcctggcgg gcgctgattg gcttttcaat tggcggatcg ctggggttga ttttgggact
     3061 gattagcggg ctatctcgct ggggagaacg gctgctggat acctcaattc agatgttgcg
     3121 caacgtaccg catctggcgc tgatcccgct ggtgattttg tggtttggca ttgatgaatc
     3181 cgcaaaaatc tttctggtgg cgctcggcac gctattcccc atttatatca acacctggca
     3241 tggcatccgt aatatcgatc gcggtctggt ggaaatggcg cgtagctatg gattatccgg
     3301 cataccgctg tttatccatg tgatcctgcc tggtgccctg ccctcaatta tggtcggcgt
     3361 gcgttttgcg ttgggcctga tgtggctgac gctgattgtt gccgaaacca tttctgccaa
     3421 ttcaggcatt ggttatctgg cgatgaatgc gcgggagttt ttgcaaacgg acgtggtggt
     3481 agtcgctatt attctttacg ccctgctcgg caaacttgct gacgtcagcg cgcagctgtt
     3541 agagcgcctc tggttgcgct ggaacccggc ttatcatttg aaggaggcca cggtatgaat
     3601 actgctcgtc tgaaccaggg cacaccattg ttgctcaatg cagtaagcaa acattacgcg
     3661 gaaaatatcg tcctgaacca actggattta catattccgg caggtcagtt tgtggcggtg
     3721 gtgggccgca gcggtggtgg caaaagtacc ctgctgcgcc tgctggccgg gctggaaacg
     3781 ccaaccgcag gcgatgtgtt ggcaggcacc acaccgctgg ctgaaattca ggaagacacg
     3841 cgaatgatgt ttcaggatgc gcgtctgcta ccgtggaaat cggtgattga taacgttggg
     3901 ttaggcctta aaggccagtg gcgcgatgcc gcgcgtcggg cgttggctgc ggtaggactg
     3961 gagaatcgcg ccggggaatg gcctgccgca ctttcaggcg ggcagaaaca gcgagtggcg
     4021 ctggcaagag cgttgattca tcgaccgggt ttattgttgc ttgatgaacc gctcggggca
     4081 ctggacgcct taacgcgact cgagatgcag gatttgattg tgtcactttg gcaggagcac
     4141 ggctttaccg tactgttggt gacgcatgat gtgagcgaag cggtagcaat ggctgaccgg
     4201 gtgttgttaa ttgaagaggg aaaaattggt ctggatttga cagtggatat cccccgtccg
     4261 cgtcgattag ggtcggtgcg attagcggag ctggaagcgg aagtattgca acgggtaatg
     4321 cggcgtgggc attctgaaca actgattcgg cgtcatggtt aatatgccgg atgtggcgca
     4381 caaaaccagc attttcaacg gactagtagg cctgataaga cacgtcaagc atcgcatcag
     4441 gcagttgggc acggctgccg gatgcggcgt gaacgcctta tccagcctac aaaaccagca
     4501 atttcaacgg agttgtaggc ctgataagac gcgtcaagcg tcgcatcagg cattttggct
     4561 caaaagtatc aagccagtgc tttggtgatc ttctcgtaca gatcgccaga gagattttcc
     4621 agccctttca actgttccag cgccgcgcgc attttctcct gacgtttggc atcataacgt
     4681 ttcaggcgaa tcagcggttc aatcagacgt gaagctacct gcgggttacg gctgttgagg
     4741 tcggtaagca tttccaccag gaactggtaa ccgctgccat cttcggcatg gaacgctgcc
     4801 gggttgctgc ccgcaaacgc accaatcaac gaacgaatac ggttcgggtt gctcatggta
     4861 aatgagcgat gctgcaacag accacgcacc gtctccagca cattcgccgc cgggctggtg
     4921 gcttgcagga taaaccattt atccatcacc agaccgtcct gatgccactt atcgtcgtac
     4981 tcctgcatca gcgcgtcacg gcaaggcagc tgtgcagcaa ccgccgcaga aagcgctgcc
     5041 agcgcatcgg tcatattgtt tgcttcatgg tattgcttgc tcaccagcac gtcagccaga
     5101 tgcgtttcac caaaagcaag gaagcgcagg caggcattac gcagagtgcg ttttgcaata
     5161 tcttcatgct caacacggta ctcgctctgg taattcgcgt tgtaaatagc cagcagctca
     5221 tccgccagtt cagtcgccag agtacgagtg agtgcttcgc gtacttcggc aatagcaatc
     5281 gggtcgatga tatcgaacag ttcagccatt tcattgacag aaggcagcgt caggatttct
     5341 gccgccagcg ccggatcaat cttctcatcg agcagcaccg cgcggaaagc gtcagccaca
     5401 tgtaccggca gagacagcgg ctgcccttgc tgatgacggg cgacgttcag cttgatgtag
     5461 gttgccagca aactttgcgc cgcatcccaa cgggagatat cattacgcgc atgacgcatc
     5521 aggaaggtca gttgctgatc gctccactta tattccagtt tcactggcgc agagaattcg
     5581 cacagcagcg caggcaccgg ctggaagtag acattatcaa aaacaaaggt ctgttccgcc
     5641 tgggtgacgt tcagcacgga attcaccgga tgaccgcctt tctgcaacgg gatcactttg
     5701 ccttcgttgt catacaattc gatggcaaat ggaatatgca gcggctgttt ttctgcctga
     5761 tccggcgtgg ctggcgtgcg ctggctgatg gtcagggtgt actgctcggt ttccggattg
     5821 tagtcgtctt tgacggtcac aatcggcgtg ccggactggc tgtaccaacg gcggaaatgg
     5881 gagagatcga cattcgacgc atcttccatc gcctgcacaa agtcgtcgca ggttgccgcg
     5941 ctgccatcat ggcgctcgaa atagagttgc atccctttct ggaagttttc ttcgcccagc
     6001 agggtgtgga tcatgcgaat cacttccgcg cccttctcgt aaacggtcag ggtgtagaag
     6061 ttgttcatct caatgaccat atccgggcgg atcgggtgcg ccatcgggct ggcgtcttcc
     6121 gcaaactgca atccgcgcat ggtgcgcaca ttattgatac ggtttaccgc gcgggaacca
     6181 aggtcagagc tgaattcctg atcgcggaag acggttaaac cttctttcag gctgagctgg
     6241 aaccagtcgc ggcaggtcac tcggttaccg gtccagttat ggaaatattc atggccgata
     6301 acgcgttcaa tatcgagata atctttgtcg gtggcggtgt cggtgcgggc caacacatat
     6361 ttggagttaa agatattcag ccccttattc tccattgcgc ccatattgaa gaaatccacc
     6421 gcgacgatca tatagatgtc gaggtcatac tccaggccaa agcgttcttc atcccatttc
     6481 atggagtttt tcagcgaggt catcgcccac ggcgcgcgat caaggttgcc gcgatcgacg
     6541 tacagctcca gtgctacttc gcgaccagaa cgcgtggtaa aggtatcgcg cagtacatca
     6601 aagtcgcctg ccaccagcgc aaacaggtag cacggtttcg ggaacgggtc ctgccactgt
     6661 acccaatggc gtccgttttc gagttcgcct tgcgcaacgc ggttaccatt ggaaagcagg
     6721 aagggatatt tgattttatc ggcaataatt ttggtggtaa aacgcgccag cacgtccggg
     6781 cggtcgagat aatacgtaat atggcggaaa ccttcggctt cacactgggt gcaaagcgca
     6841 tcgcctgact gataaagccc ttccagcgcc gtattcgccg ccgggcttat ttcattaatg
     6901 atcttaagcg taaaacgctc cggcaaatta ctgatgacca gtgcgccctc ttcttctttc
     6961 caggcggtcc acagctcatc attaatatga acagaaacca gtttgaggtc ttcgccgttg
     7021 agacgaagcg gagcatctga tgcaccatga cggacagcct ggctgaccgc ggtaacgacc
     7081 gtcttttgcg cgtcgaggtc aaaggtcaag tcaatatcag taatctggta atccggcgca
     7141 cgataatcgt gacggtattt ggcttgtggc tgttgagtca taaataacct ttagcatctt
     7201 ttatagagtc tggtgttcag tctattcctg ttgcgtaaat cgcgctatgc agaatcttca
     7261 tcttttcagg tacaaacgcc tttattgcta catttttata acatacaccg cgtaatgcca
     7321 tcgaccagaa aggtggcata tggtgtgatc ggggttcaat aaattgcgaa acaaggtata
     7381 ctccagcagt tcctgaagat gtttattgta ctaaacgctc ctgtacgagg acgctactgc
     7441 gcacctatga cacaattcgc ttctcctgtt ctgcactcgt tgctggatac agatgcttat
     7501 aagttgcata tgcagcaagc cgtgtttcat cactattacg atgtgcatgt cgcggcggag
     7561 tttcgttgcc gaggtgacga tctgctgggt atttatgccg atgctattcg tgaacagatt
     7621 caggcgatgc agcacctgcg cctgcaggat gatgaatatc agtggctctc tgctctgcct
     7681 ttctttaagg ccgactatct taactggtta cgcgagttcc gctttaaccc ggaacaagtc
     7741 accgtatcca acgataatgg caagctggat attcgtttaa gcggcccgtg gcgtgaagtc
     7801 atcctctggg aagttccttt gctggcggtt atcagtgaaa tggtacatcg ctatcgctca
     7861 ccgcaggccg acgttgcgca agccctcgac acgctggaaa gcaaattagt cgacttctcg
     7921 gcgttaaccg ccggccttga tatgtcgcgc ttccatctta tggattttgg cacccgtcgc
     7981 cgtttttctc gcgaagtaca agaaaccatc gttaagcgtc tgcaacagga atcctggttc
     8041 gtgggcacca gcaactacga tctggcgcgt cggctttccc tcacgccgat gggaacacag
     8101 gcacacgaat ggttccaggc acatcagcaa atcagcccgg atctagccaa cagccagcga
     8161 gctgcacttg ctgcctggct ggaagaatat cccgaccaac ttggcattgc attaaccgac
     8221 tgcatcacta tggatgcttt cctgcgtgat ttcggtgtcg agttcgccag tcgctatcag
     8281 ggcctgcgtc atgactctgg cgacccggtt gaatggggtg aaaaagccat tgcacattat
     8341 gaaaagctgg gaattgatcc acagagtaaa actctggttt tctctgacaa tctggattta
     8401 cgcaaagcgg ttgagctata ccgccacttc tcttcccgcg tgcaattaag ttttggtatt
     8461 gggactcgcc tgacctgcga tatcccccag gtaaaacccc tgaatattgt gattaagttg
     8521 gttgagtgta acggtaaacc ggtggcgaaa ctttctgaca gccctggcaa aactatctgc
     8581 cacgataaag cgtttgttcg ggcgctgcgc aaagcgttcg accttccgca tattaaaaaa
     8641 gccagttaat atcatcaggg agctaatcgg ctcccttttt ttacctttaa ttccgaaatc
     8701 tttcgctgca tttgcgaatt ctgcttgtct gattgcagat gccaggtaac ataggtatcc
     8761 ccccatttac gggtggacaa gtgtttattt tttccgacta ttaacagaga gaatattatg
     8821 agcgttgtgc ctgtagccga cgtactccag ggccgcgtag ccgttgacag cgaagtcacc
     8881 gtacgcggat gggtacgtac ccgccgagat tcaaaagctg gcatctcctt cctcgccgtt
     8941 tatgacggtt cctgctttga tcctgtacag gctgtcatca ataattctct gcccaattac
     9001 aatgaagacg tcctgcgtct gaccaccggc tgctcggtca ttgtgacggg taaagtcgtg
     9061 gcgtcgccgg gccaggggca acaatttgaa attcaggcca gcaaagttga agttgctggt
     9121 tgggttgaag atccagacac ttacccgatg gcggcaaaac gccacagcat tgagtatctg
     9181 cgtgaagtcg ctcacctgcg tccgcgcaca aacctgattg gtgccgtcgc gcgcgttcgc
     9241 catacgctgg cgcaggcgct gcatcgcttc tttaacgagc agggattctt ctgggtttca
     9301 acgccactga ttaccgcatc tgataccgaa ggtgcaggcg aaatgttccg cgtttctacg
     9361 ctggatctgg aaaacctgcc gcgtaacgat cagggcaaag tggatttcga caaagacttc
     9421 tttggtaaag agtctttcct gaccgtatct ggccagttga acggcgaaac ctacgcttgc
     9481 gcattatcca aaatttatac cttcggcccg actttccgtg ctgaaaactc caacaccagc
     9541 cgtcacctgg cggaattctg gatgctggag ccggaagtgg cgtttgctaa cctgaacgat
     9601 atcgcgggtc tggctgaagc catgctgaaa tatgtcttca aagcggttct cgaagaacgc
     9661 gcagacgaca tgaaattctt cgctgaacgc gtagataaag atgccgtttc acgtctggaa
     9721 cgcttcattg aggccgattt cgcgcaggtg gattacaccg acgcagtgac cattctcgaa
     9781 aactgcggca ggaagtttga aaacccggtt tactggggcg tcgatctctc ttctgagcat
     9841 gagcgttatc tggcggaaga acactttaaa gcaccggtag tggttaaaaa ctatccgaaa
     9901 gatattaaag cgttctatat gcgcctcaac gaagacggta aaaccgttgc ggctatggac
     9961 gttctggctc cgggcatcgg tgagatcatt ggtggctccc agcgtgaaga acgtctggac
    10021 gttctggacg agcgtatgct ggaaatgggc ctgaataaag aagattactg gtggtatcgc
    10081 gatctgcgtc gctacggtac tgttccgcat tcaggtttcg gtcttggttt tgaacgcctg
    10141 attgcttacg taactggcgt gcaaaacgtg cgtgatgtga ttccgttccc acgtactccg
    10201 cgcaacgcca gcttctgatt ttcttcctca aagagccagc gcctgctggc tcttttttat
    10261 cctttctgtc aagttatctg tttgttaagt caagcaatct atttgcaacc ccgccataac
    10321 gatcatcctg ttacggaata ttacattgca acatttacgc acaaaaaata acccgcattc
    10381 ttattgcgga ttagtttttt cttagctgat agcacaattt tcatac
//
LOCUS       DAZGVQ010000169        10299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000169 DAZGVQ010000000
VERSION     DAZGVQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.170"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..305
                     /gene="trbF"
                     /locus_tag="ACHLAG_004299"
     CDS             <1..305
                     /gene="trbF"
                     /locus_tag="ACHLAG_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001700179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HFP0657424.1"
                     /translation="GLPLVFLTAGIVLWTAMNIIFDACIDSWKMDQELNNSIYMWNIL
                     IYVIPYALYALAVGFLVTFFLFPMYELIYGNIGIFLLKRRMRRENTLREGSNNASH"
     gene            292..1665
                     /gene="traH"
                     /locus_tag="ACHLAG_004300"
     CDS             292..1665
                     /gene="traH"
                     /locus_tag="ACHLAG_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFP0657425.1"
                     /translation="MLRIKPLFILCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKASDKDKERVTKNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGG
                     TAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGF
                     ISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYD
                     EAVIGHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            1662..4478
                     /gene="traG"
                     /locus_tag="ACHLAG_004301"
     CDS             1662..4478
                     /gene="traG"
                     /locus_tag="ACHLAG_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
                     /protein_id="HFP0657426.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTWTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVKVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSVTYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDNNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASQIMRQNV
                     TMNALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSIGHVTMRNLPMVQTILTG
                     IAIGIFPLLILAAVFNKLTLSVLKGYVFALMWLQTWPLLYAILNSAMTFYAKQNGAPV
                     VLSELSQIQLKYSDLASTAGYLSAMIPPLSWMMVKGLGAGFSSVYSHFASSSISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNTLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATRELASRSTNASLGLY
                     GDAYAKGHLGISVLGNGGGVGLQAGAKASIDGSDLDSHEASSGSRASHDARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKNAPQDVEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNTWRESRRDIGKGMESVPSGGGSQDIIADHQGHQAIIEQRTQDSNIRND
                     VKHQVDNMVTEYRGNIGDTQNSIRGEENIVKGQYSELQNHHKTEALTQNNKYNEEKLA
                     QERIPGADSPKELLEKAKSYQHKK"
     gene            4511..5032
                     /gene="traS"
                     /locus_tag="ACHLAG_004302"
     CDS             4511..5032
                     /gene="traS"
                     /locus_tag="ACHLAG_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632670.1"
                     /note="TraS, as the term is used in F-like plasmids, is a
                     plasmid entry exclusion protein. It interacts with the
                     F-like plasmid mating stabilization protein TraG; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer entry exclusion protein TraS"
                     /protein_id="HFP0657427.1"
                     /translation="MITQQIISSELEVLKKHIDSGDIRIPSLWQGLKPGLIIMGWMIF
                     CPLLMSFLITQKTSETLTAVLAGGWLGLIILFIVARIRMLYFSLPEEFLKTSSVMRVI
                     SSKLKVYFIVYMGVIFLWSFLGGGIIYGFGAILVTVIMAFLIQLDIGRYQFVGVIDAI
                     NSYVKNKKLSRVK"
     gene            5057..5788
                     /gene="traT"
                     /locus_tag="ACHLAG_004303"
     CDS             5057..5788
                     /gene="traT"
                     /locus_tag="ACHLAG_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFP0657428.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            6041..8257
                     /gene="traD"
                     /locus_tag="ACHLAG_004304"
     CDS             6041..8257
                     /gene="traD"
                     /locus_tag="ACHLAG_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001577029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFP0657429.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWIKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCSEQLWSAFVLAAVVALVICLITFFVVSWILGRQGKQQSENEATGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PQQPQQPQQPQQPQQPQQPQQPMSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQ
                     LPPGISESGEVVDMAAYEAWQQENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            8257..10251
                     /gene="mobF"
                     /locus_tag="ACHLAG_004305"
                     /pseudo
     CDS             8257..10251
                     /gene="mobF"
                     /locus_tag="ACHLAG_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobF family relaxase"
BASE COUNT         2625 a         2388 c         2906 g         2380 t
ORIGIN      
        1 ctggattgcc tctggtattt ctgactgccg gaattgtttt atggacagct atgaatatta
       61 tttttgatgc ctgtattgat tcgtggaaaa tggaccaaga attaaataat tcgatttata
      121 tgtggaatat cctgatatat gtcattccgt atgcgttata tgcgcttgca gtcgggtttc
      181 ttgttacatt ttttctgttc ccaatgtacg aattaatata cggaaatatc gggatattcc
      241 tgttgaaacg ccgtatgcgc cgggagaaca cattaaggga gggcagcaat aatgcttcgc
      301 attaagcctc tttttattct ttgtgccgcc ttactgacgg tgacgcctgc agcgtcagcg
      361 gatgtgaaca gcgacatgaa tcagttcttt aacaagctgg gcttcgcctc caataccaca
      421 cagccgggcg tctggcaggg gcaggctgcc ggctatgctt acggcggctc cctgtatgcc
      481 cgtactcagg tcaaaaatgt tcagctgatt tccatgacgc tgccggatat caatgccgga
      541 tgtggcggca tcgacgctta tctcggttcg ttcagtttta ttaatggtga gcagctgcag
      601 cgttttgtta agcagattat gagtaatgct gccggttact tttttgacct tgccctgcag
      661 acaacggtgc cggaaatcaa aaccgcaaaa gacttcctgc agaaaatggc aagcgacatt
      721 aacagcatga acctcagttc ctgccaggcg gcgcagggga ttatcggcgg gcttttcccc
      781 cggacgcagg tgtcccagca aaaagtctgt caggacattg ccggtgagag caatattttt
      841 gctgactggg cggcttcccg gcagggatgt accgttggcg gtaaatctga cagcgtcagg
      901 gataaagcca gcgacaagga taaggagcgg gtgaccaaaa acattaacat catgtggaat
      961 gcgctttcca aaaacagaat gtttgacggc aacaaagagc tgaaagagtt tgtgatgacg
     1021 ctgaccggct cactggtgtt tggtcccaac ggcgaaatca caccgctgtc agccagaacc
     1081 actgaccgct caattatccg ggccatgatg gaaggcggca cagcaaaaat ttatcactgc
     1141 aacgattctg ataaatgcct gaaagtggtg gcagacacac cggtgaccat cagccgggat
     1201 aatgcactga agtctcagat tactaaactt ctggccagca ttcagaacaa ggctgtcagt
     1261 gacacgcctc tggatgacaa ggaaaaaggc tttatttcca gtaccaccat ccccgtcttc
     1321 aaatacctgg ttgacccgca gatgctcggt gtttccaaca gtatgattta ccagctgacg
     1381 gactatatcg gttacgacat cctgctgcag tacattcagg agctgataca gcaggcccgg
     1441 gcgatggtgg ccacgggaaa ttatgacgaa gcggttatcg ggcatattaa cgacaacatg
     1501 aatgatgcca cccggcagat tgcggcgttt cagtcacagg tgcaggtaca gcaggatgcg
     1561 ctgctggttg tcgatcgtca gatgagctac atgcgtcagc agctttccgc ccgcatgctc
     1621 agtcgttacc agaacaacta tcacttcgga gggagcacgc tgtgaatgaa gtttatgtga
     1681 ttgccggtgg tgagtggttg cggaataacc tgaacgccat tgccgccttt atgggaacct
     1741 ggacgtggga ttccattgaa aaaattgcgc tcacattgtc tgttctcgcg gtggccgtaa
     1801 tgtgggtaca gcggcacaac gtgatggatt tgctgggctg ggtggccgtg tttgtgctta
     1861 tcagcctgct ggttaatgtc cgcacatcgg tgcagattat tgataacagt gacctggtca
     1921 aagttcaccg ggtggataat gtgccggtcg gtctggcgat gccactttca ctgacgaccc
     1981 gtatcgggca tgcaatggtg gccagttacg agatgatctt cacgcaaccg gacagcgtca
     2041 cctacagcaa aacggggatg ctgttcgggg cgaatctgat tgtgaaaagc accgatttcc
     2101 tgtcccggaa tccggaaatc atcaatctgt tccaggacta cgtccagaac tgcgtgctgg
     2161 gtgatattta cctgaatcat aaatacacgc tggaagacct gatggcctcc gctgacccct
     2221 acacgctgat tttttcccgc cccagcccgc tacggggcgt ttatgacaac aataataatt
     2281 tcataacttg taaggatgcg tcggtcacgc tgaaagacag gctgaatctc gatacaaaga
     2341 cgggaggcaa gacctggcat tattatgtgc agcagatatt tggcggcaga ccggacccgg
     2401 acctgttatt cagacaactg gtcagtgaca gttacagtta tttctacggc tccagtcagt
     2461 ctgccagcca gattatgcgc cagaacgtca ccatgaatgc cctgaaagag ggtatcacca
     2521 gtaatgcagc ccgtaacggt gacaccgcca gcctggtaag tctggccacc acgtcatcga
     2581 tggagaaaca acgtctggca catgtctcca ttggtcatgt aactatgcga aacctgccga
     2641 tggtccagac tatcctgacg gggattgcca ttggtatatt cccgctcctg atactggcgg
     2701 ctgtctttaa caagctgaca ttgtctgtat taaaggggta tgtgtttgcc ctgatgtggc
     2761 tgcagacctg gccgttgttg tatgccattc tgaacagtgc catgacattc tatgcgaaac
     2821 agaatggtgc gccggtcgtg ttgtctgaac tctctcagat acagctgaaa tactctgacc
     2881 tggcctccac tgccgggtat ctttcagcca tgatcccccc gttgtcatgg atgatggtaa
     2941 aaggccttgg cgcaggtttt tccagcgtgt acagccactt cgcctcttca tctatcagtc
     3001 caacggccag tgcagcaggc agtgtggttg acggtaatta ctcctacggc aacatgcaga
     3061 cggaaaacgt gaacggcttc agctggagca ccaacagcac cacgtcgttt ggtcagatga
     3121 tgtaccagac cggcagtggc gcaaccgcca cacagacccg tgacggtaat atggtgatgg
     3181 atgcaagcgg agcgatgtcc cggttaccgg tcggtatcaa tgcaacgcgt cagattgcag
     3241 cggcacaaca ggaaatggcc cgggaggcgt cgaacagagc cgaaagtgcc ctgcatggtt
     3301 tcagcagcag tattgccagt gcctggaaca cactcagtca gtttggctct aaccggggga
     3361 gcagtgactc tgtcactggt ggtgctgaca gcacgatgag tgctcaggac tccatgatgg
     3421 ccagccgtat gcgcagtgca gtggaaagct atgcgaaggc gcataatatc agtaatgagc
     3481 aggcgacgag agaactggcc tcgagaagta caaatgcgtc gcttggcctt tacggtgatg
     3541 cttatgctaa agggcattta ggcataagtg tattgggtaa cggtggaggg gttggtctcc
     3601 aggccggagc taaggcaagc attgatggca gtgatcttga ctcacatgaa gccagtagtg
     3661 gttcacgggc cagccatgat gctcgtcatg atatcgatgc cagagctaca caagacttta
     3721 aagaagccag cgattacttt accagccgca aagtcagtga atccggcagc catactgaca
     3781 ataatgctga ttcccgtgtg gatcagttgt ctgcagccct gaattcagcg aaacagagtt
     3841 acgaccagta cacgacgaat atgacccgca gccatgaata tgctgaaatg gcatcccgta
     3901 ctgagagtat gagtgggcag atgagtgaag atctgtcgca acagtttgca cagtatgtga
     3961 tgaaaaacgc accgcaggat gttgaagcta ttctgacaaa taccagttcg ccggagattg
     4021 cagaacgccg tcgggctatg gcgtggtctt ttgtgcagga acaggtacag cctggtgttg
     4081 ataacacctg gcgtgaatcc cgtagagata taggtaaagg aatggagagc gtaccttcgg
     4141 gtggtggcag ccaggatatt attgctgatc atcagggaca tcaggccatt attgagcaaa
     4201 gaacgcagga cagtaatatc cgtaatgatg taaaacatca ggttgataat atggtcacag
     4261 aatatcgagg taatatcgga gatactcaga acagcatccg aggcgaggaa aatattgtaa
     4321 aagggcagta ttctgaacta caaaatcatc ataaaacaga agcattaact cagaataata
     4381 aatataatga agaaaagctg gctcaggaaa gaatacctgg tgctgatagt ccgaaagagc
     4441 ttttagagaa agcaaagagt taccagcata aaaaataatc ataacatgtt gggtagggta
     4501 tggagagacc atgattacac aacaaattat cagtagtgag ttggaagttt taaagaaaca
     4561 tattgactca ggagatatta gaattccgtc actatggcag ggattaaaac ccgggcttat
     4621 catcatgggg tggatgattt tttgtccctt gctaatgtca tttctaataa cacaaaagac
     4681 aagtgaaaca ctcactgctg tgttagccgg aggatggctt ggacttatta ttctgtttat
     4741 tgtggcaagg attagaatgt tatatttctc tctgccggaa gaattcctaa agacatcatc
     4801 cgtaatgagg gttatatcat caaaacttaa agtgtatttc attgtgtata tgggggttat
     4861 atttttatgg tcttttttag gtggagggat tatatacggt tttggcgcaa ttttagtgac
     4921 ggttattatg gcgttcttga ttcaattaga tatcggtaga tatcaatttg ttggtgttat
     4981 tgatgcaatc aatagttacg tcaaaaacaa gaagttatca agagtaaaat aaaagatatt
     5041 agagagtaaa tatatgatga aaacaaaaaa actaatgatg gttgcactgg tcagttccac
     5101 tctggccctt tcagggtgtg gtgcgatgag cacagcaatc aagaagcgta accttgaggt
     5161 gaagactcag atgagtgaga ccatctggct tgaacccgcc agcgaacgca cggtatttct
     5221 gcagatcaaa aacacgtctg ataaagacat gagtgggctg cagggcaaaa ttgctgatgc
     5281 tgtgaaagca aaagggtatc aggtggtgac ttctccggat aaagcctact actggattca
     5341 ggcgaatgtg ctgaaggccg ataagatgga tctgcgggag tctcagggat ggctgaaccg
     5401 tggttatgaa ggcgcagcag ttggtgcagc gttaggtgcc ggtatcaccg gatataattc
     5461 caattctgcc ggtgccacac tcggtgtggg ccttgctgcc ggtctggtgg gtatggctgc
     5521 agatgcgatg gtggaagatg tgaactatac catgatcacg gatgtgcaga ttgcagagcg
     5581 tactaaggca acggtgacaa cggataatgt tgccgccctg cgtcagggca catcaggtgc
     5641 gaaaattcag accagtacag aaacaggtaa ccagcataaa taccagaccc gtgtggtctc
     5701 aaatgcgaac aaggttaacc tgaaatttga agaggcgaag cctgttctcg aagaccagct
     5761 ggccaaatca atcgcaaata ttctctgatt atcttccgga ggctggtctg accggcctcc
     5821 tgactgacgc ctcgctttgc tcgttgtcca accccggaaa gaaataaaat aaattttatt
     5881 tctccccggc gtttccgaaa taaatatgaa aacactgagc ggttgtgcaa attctttgtg
     5941 cgaccgtgaa gtgttgccgg cgaagccgga atttaacggt tattaatctt ttcatcatta
     6001 ataatcagtc cgactgactt tttttcttcg gaatatcatc atgagtttta acgcaaagga
     6061 tatgacccag ggcggtcaga ttgcgtccat gcgtatccgc atgttcagcc agatcgccaa
     6121 tatcatgctt tactgcctgt ttattttttt ctggatactc gttggtctgg ttttatggat
     6181 aaaaataagc tggcagacgt ttgtgaacgg ctgtatttac tggtggtgta ccacgctgga
     6241 aggcatgcgg gatttaatca aatcccagcc ggtctatgag atccagtatt acggtaaaac
     6301 cttccgcatg aacgctgctc aggtactgca tgacaaatat atgatctggt gcagtgagca
     6361 gttatggtcc gccttcgtgc tggctgctgt tgtggcactg gttatttgcc tgattacctt
     6421 ctttgttgtc tcctggattc tggggcgtca gggtaaacaa cagagcgaaa atgaagccac
     6481 aggtggtcgt cagctgacag acaatccgaa agacgttgcc cggatgctga aaaaagacgg
     6541 caaggactcc gatatccgga ttggagacct gccgattatc cgggattccg aaatccagaa
     6601 cttctgtctg cacggcacgg tcggggccgg taagtcggaa gttatccgtc gtctggccaa
     6661 ctacgcccgt cagcgcggtg atatggtggt gatttatgac cgttcggggg aatttgttaa
     6721 aagttactat gatccctcca tcgataaaat cctgaatccg ctggatgccc gctgtgccgc
     6781 ctgggattta tggaaggagt gtctgacaca gccggatttt gataatacgg caaataccct
     6841 gatcccgatg ggcacaaaag aggacccgtt ctggcagggt tcaggacgta ccatttttgc
     6901 ggaagcggcg tacctgatgc gtaatgaccc caaccgtagc tacagcaaac tggtggacac
     6961 actgctttcc atcaaaatcg aaaaactgcg taccttcctg cgtaattcac cggcggccaa
     7021 cctggtggaa gagaaaattg agaaaacggc gatttccatc cgtgctgtgc tgaccaacta
     7081 cgtgaaagcc atccgttacc tgcaggggat tgagcataac ggtgagtctt ttaccatccg
     7141 tgactggatg cggggtgtcc gggaagatca gaaaaacggc tggctgttta tctcgtcgaa
     7201 tgccgacacc catgcctccc tgaagccggt gatctccatg tggctgtcca ttgccattcg
     7261 tggtctgctg gcaatgggag aaaaccgtaa ccgtcgtgtg tggtttttct gtgatgagct
     7321 acccacgtta cacaaactgc cggacctggt ggagatcctg ccggaagccc gtaagttcgg
     7381 tggctgttat gtgtttggta tccagtccta tgcccagctg gaagatatct acggtgagaa
     7441 agcggctgcc acgctgtttg acgtcatgaa cacccgtgcc tttttccgtt ctcccagcca
     7501 taagattgca gagttcgctg ccggtgagat tggtgagaaa gagcacctga aagccagcga
     7561 gcagtactcc tacggtgctg atccggtacg tgacggggta tcgaccggta aggatatgga
     7621 gcgccagacg ctggtcagtt attccgacat tcagtctctg ccggatttga cctgttatgt
     7681 caccctgccc ggaccgtatc cggcagtaaa actctcactg aaatatcagg cacgtccgaa
     7741 ggttgcccct gagtttattc cgcgtgacat caacccggaa atggagaacc gcctgagcgc
     7801 cgtacttgcc gcaagggaag cagaaggtcg tcagatggcc agcctcttcg aaccggatgt
     7861 gccggaagtt gtttccggag aagacgtgac tcaggctgaa cagccgcaac agccgcaaca
     7921 gccgcaacag ccgcaacagc cgcaacagcc gcaacagccg caacagccgc aacagccgat
     7981 gtctcctgcc atcaacgata agaagtcaga ttcaggtgtg aatgttccgg caggggggat
     8041 cgagcaggag ctgaaaatga aaccggaaga agagatggaa cagcaactgc cacccgggat
     8101 cagtgaatcc ggtgaagtgg tggatatggc cgcttatgag gcatggcaac aggaaaatca
     8161 tccggacatc cagcagcaga tgcagcgtcg tgaagaggtg aacattaatg tgcaccggga
     8221 gcgcggggag gatgttgagc cgggagatga tttctgatga tgagtatcgc gcaggtcaga
     8281 tcggccggca gtgccgggaa ctattatacc gacaaggata attactatgt gctgggcagc
     8341 atgggagaac gctgggccgg caggggggct gaacagctgg gactgcaggg cagtgtcgat
     8401 aaggatgttt ttacccgtct tctggaggga aggctgccgg acggagcgga tctaagccgc
     8461 atgcaggatg gcagtaacag gcatcgtccc ggctacgacc tgaccttctc cgcccccaaa
     8521 agtgtctcca tgatggccat gttaggtggc gataagcgcc tgattgatgc acataaccag
     8581 gccgtggact ttgctgttcg tcaggtggag gcgctggcct ccacacgggt gatgacggac
     8641 ggacagtcag aaacggtgct gaccggtaat ctggtgatgg cactgtttaa ccacgacacc
     8701 agtcgcgatc aggaaccaca gttacacacg catgcggtgg tggctaatgt cacgcagcat
     8761 aacggcgaat ggaagacact gagcagtgac aaagtgggga aaacggggtt cattgagaat
     8821 gtgtacgcta atcagattgc ctttggcagg ctctaccggg aaaaactgaa agagcaggtt
     8881 gaggcgctgg gctatgaaac cgaagtggtg ggtaagcacg gtatgtggga aatgccgggc
     8941 gtaccggtgg aggccttttc cggacgctca caggcaatcc gggaggccgt cggggaagat
     9001 gcctcgctga aatcccggga tgtggcggca ctggatacgc gaaaatccaa acagcacgtc
     9061 gatcctgaag tcagaatggc tgaatggatg cagacgctga aggaaaccgg gttcgacatc
     9121 cgggcatatc gtgacgcggc ggatcagcgt gcggagaccc gcacgcaggc gcccggggct
     9181 gtttcacagg aggggccgga tgtgcagcag gcggtgacac aggccattgc cggattaagt
     9241 gaacgaaaag tgcagtttac gtacacggac gtgctggcca gaacggtcgg catactgccg
     9301 ccggaaaatg gtgtgattga acgggcacgc gccggtatcg atgaggccat cagccgtgag
     9361 cagcttatcc ccctcgatcg tgagaagggg ctgttcacgt ccgggattca tgtgctcgat
     9421 gagctgtcag tccgggcact cagtcgtgac atcatgaaac agaaccgggt gaccgtacat
     9481 ccggagaaaa gtgtcccccg gacggctggt tacagcgatg cggtcagtgt tctggcacag
     9541 gaccgtccgt cgctggccat tgtgtccggg cagggcggtg cagccgggca gcgtgagcgg
     9601 gtggctgaac tggtcatgat ggcccgggag caggggcggg aggtgcagat tatcgctgct
     9661 gaccgtcgct cgcagatgaa cctgaagcag gatgaacggt tgtccggtga gctgataacc
     9721 ggacgtcgtc agctgctgga aggcatggcc ttcacgccgg gcagtactgt tatcgttgac
     9781 cagggcgaaa aactctccct gaaagagacg ttaaccctgc tggacggtgc tgcacgtcat
     9841 aacgtacagg tcctgataac cgacagcggg cagcgaaccg gtacaggcag tgcactgatg
     9901 gccatgaagg atgccggggt gaacacatat cgctggcagg ggggagaaca gcgaccggcc
     9961 accatcatca gtgaaccgga ccgtaatgtc cgctatgccc ggctggcagg agattttgcg
    10021 gtcagcgtga aagccggaga agagagcgtg gcacaggtca gcggggtacg ggaacaggcc
    10081 atactgacac aggccattcg cagtgagctg aaaacacagg gcgtgctcgg acacccggag
    10141 gtaaccatga ccgccctgtc accggtctgg ctggacagcc ggagccgtta tctgcgggat
    10201 atgtaccgtc cggggatggt gatggagcag tggaacccgg agacacgcag tatagcgcgg
    10261 cgattttttt gataccgatt tgcatttcat ttgataccg
//
LOCUS       DAZGVQ010000170        10297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000170 DAZGVQ010000000
VERSION     DAZGVQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.171"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            132..503
                     /gene="rplN"
                     /locus_tag="ACHLAG_004306"
     CDS             132..503
                     /gene="rplN"
                     /locus_tag="ACHLAG_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFP0657430.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            514..828
                     /gene="rplX"
                     /locus_tag="ACHLAG_004307"
     CDS             514..828
                     /gene="rplX"
                     /locus_tag="ACHLAG_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFP0657431.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            843..1382
                     /gene="rplE"
                     /locus_tag="ACHLAG_004308"
     CDS             843..1382
                     /gene="rplE"
                     /locus_tag="ACHLAG_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFP0657432.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            1397..1702
                     /gene="rpsN"
                     /locus_tag="ACHLAG_004309"
     CDS             1397..1702
                     /gene="rpsN"
                     /locus_tag="ACHLAG_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP0657433.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            1736..2128
                     /gene="rpsH"
                     /locus_tag="ACHLAG_004310"
     CDS             1736..2128
                     /gene="rpsH"
                     /locus_tag="ACHLAG_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFP0657434.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            2141..2674
                     /gene="rplF"
                     /locus_tag="ACHLAG_004311"
     CDS             2141..2674
                     /gene="rplF"
                     /locus_tag="ACHLAG_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFP0657435.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            2684..3037
                     /gene="rplR"
                     /locus_tag="ACHLAG_004312"
     CDS             2684..3037
                     /gene="rplR"
                     /locus_tag="ACHLAG_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFP0657436.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            3052..3555
                     /gene="rpsE"
                     /locus_tag="ACHLAG_004313"
     CDS             3052..3555
                     /gene="rpsE"
                     /locus_tag="ACHLAG_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFP0657437.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            3559..3738
                     /gene="rpmD"
                     /locus_tag="ACHLAG_004314"
     CDS             3559..3738
                     /gene="rpmD"
                     /locus_tag="ACHLAG_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFP0657438.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            3742..4176
                     /gene="rplO"
                     /locus_tag="ACHLAG_004315"
     CDS             3742..4176
                     /gene="rplO"
                     /locus_tag="ACHLAG_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFP0657439.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            4184..5515
                     /gene="secY"
                     /locus_tag="ACHLAG_004316"
     CDS             4184..5515
                     /gene="secY"
                     /locus_tag="ACHLAG_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFP0657440.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            5547..5663
                     /gene="rpmJ"
                     /locus_tag="ACHLAG_004317"
     CDS             5547..5663
                     /gene="rpmJ"
                     /locus_tag="ACHLAG_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFP0657441.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            5810..6166
                     /gene="rpsM"
                     /locus_tag="ACHLAG_004318"
     CDS             5810..6166
                     /gene="rpsM"
                     /locus_tag="ACHLAG_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFP0657442.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            6183..6572
                     /gene="rpsK"
                     /locus_tag="ACHLAG_004319"
     CDS             6183..6572
                     /gene="rpsK"
                     /locus_tag="ACHLAG_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFP0657443.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            6606..7226
                     /gene="rpsD"
                     /locus_tag="ACHLAG_004320"
     CDS             6606..7226
                     /gene="rpsD"
                     /locus_tag="ACHLAG_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFP0657444.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            7252..8241
                     /gene="rpoA"
                     /locus_tag="ACHLAG_004321"
     CDS             7252..8241
                     /gene="rpoA"
                     /locus_tag="ACHLAG_004321"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFP0657445.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            8282..8665
                     /gene="rplQ"
                     /locus_tag="ACHLAG_004322"
     CDS             8282..8665
                     /gene="rplQ"
                     /locus_tag="ACHLAG_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFP0657446.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            8772..9140
                     /locus_tag="ACHLAG_004323"
     CDS             8772..9140
                     /locus_tag="ACHLAG_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFP0657447.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            9151..9576
                     /gene="zntR"
                     /locus_tag="ACHLAG_004324"
     CDS             9151..9576
                     /gene="zntR"
                     /locus_tag="ACHLAG_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFP0657448.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            9632..9850
                     /gene="arfA"
                     /locus_tag="ACHLAG_004325"
     CDS             9632..9850
                     /gene="arfA"
                     /locus_tag="ACHLAG_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFP0657449.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(9847..10257)
                     /gene="mscL"
                     /locus_tag="ACHLAG_004326"
     CDS             complement(9847..10257)
                     /gene="mscL"
                     /locus_tag="ACHLAG_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFP0657450.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
BASE COUNT         2601 a         2397 c         2740 g         2559 t
ORIGIN      
        1 cagaaatgag ccgtttattt tttctaccca tatccttgaa gcggtgttat aatgccgcgc
       61 cctcgatatg gggattttta acgacctgat tttcgggtct cagtagtagt tgacattagc
      121 ggagcactaa aatgatccaa gaacagacta tgctgaacgt cgccgacaac tccggtgcac
      181 gtcgcgtaat gtgtatcaag gttctgggtg gctcgcaccg tcgctacgca ggcgtaggcg
      241 acatcatcaa gatcaccatc aaagaagcaa ttccgcgtgg taaggtcaaa aaaggtgatg
      301 tgctgaaggc ggtagtggtg cgcaccaaga agggtgttcg tcgcccggac ggttctgtca
      361 ttcgcttcga tggtaatgct tgtgttcttc tgaacaacaa cagcgagcag cctatcggta
      421 cgcgtatttt tgggccggta actcgtgagc ttcgtagtga gaagttcatg aaaattatct
      481 ctctggcacc agaagtactc taaggagcga atcatggcag cgaaaatccg tcgtgatgac
      541 gaagttatcg tgttaaccgg taaagataaa ggtaaacgcg gtaaagttaa gaatgtcctg
      601 tcttccggca aggtcattgt tgaaggtatc aacctggtta agaaacatca gaagccggtt
      661 ccggccctga accaaccggg tggcatcgtt gaaaaagaag ccgctattca ggtttccaac
      721 gtagcaatct tcaatgcggc aaccggcaag gctgaccgtg taggctttag attcgaagac
      781 ggcaaaaaag tccgtttctt caagtctaac agcgaaacta tcaagtaatt tggagtagta
      841 cgatggcgaa actgcatgat tactacaaag acgaagtagt taaaaaactc atgactgagt
      901 ttaactacaa ttctgtcatg caagtccctc gggtcgagaa gatcaccctg aacatgggtg
      961 ttggtgaagc gatcgctgac aaaaaactgc tggataacgc agcagcagac ctggcagcaa
     1021 tctccggtca aaaaccgctg atcaccaaag cacgcaaatc tgttgcaggc ttcaaaatcc
     1081 gtcagggcta tccgatcggc tgtaaagtaa ctctgcgtgg cgaacgcatg tgggagttct
     1141 ttgagcgcct gatcactatt gctgtacctc gtatccgtga cttccgtggc ctgtccgcta
     1201 agtctttcga cggtcgtggt aactacagca tgggtgtccg tgagcagatc atcttcccag
     1261 aaatcgacta cgataaagtc gaccgcgttc gtggtttgga tattaccatt accactactg
     1321 cgaaatctga cgaagaaggc cgcgctctgc tggctgcctt tgacttcccg ttccgcaagt
     1381 aaggtagggt tactaaatgg ctaagcaatc aatgaaagca cgcgaagtaa aacgcgtagc
     1441 tttagctgat aaatacttcg cgaaacgcgc tgaactgaaa gcgatcatct ctgatgtgaa
     1501 cgcttccgac gaagatcgtt ggaacgctgt tctcaagctg cagactctgc cgcgtgattc
     1561 cagcccgtct cgtcagcgta accgctgccg tcaaacaggt cgtccgcatg gtttcctgcg
     1621 gaagttcggg ttgagccgta ttaaggtccg tgaagccgct atgcgcggtg aaatcccggg
     1681 tctgaaaaag gctagctggt aattgtcacc aattgaatca cgggagtaaa gacagatgag
     1741 catgcaagat ccgatcgcgg atatgctgac ccgtatccgt aacggtcagg ccgcgaacaa
     1801 agctgcggtc accatgcctt cctccaagct gaaagtggca atcgccaacg tgctgaagga
     1861 agaaggtttt attgaggatt ttaaagttga aggcgacacc aagcctgaac tggaacttac
     1921 tctgaagtat ttccagggca aagctgttgt agaaagcatt cagcgtgtca gccgcccagg
     1981 tctgcgcatc tataaacgta aagatgagct gccgaaagtt atggcgggtc tgggtatcgc
     2041 agttgtttct acctctaaag gtgttatgac tgatcgtgca gcgcgccagg ctggtcttgg
     2101 tggcgaaatt atctgctacg tagcctaatc ggaggaaaaa atgtctcgtg ttgctaaagc
     2161 accggtcgtt gttcctgccg gcgttgacgt aaaaatcaac ggtcaggtta ttacgatcaa
     2221 aggtaaaaac ggcgagctga ctcgtactct caacgatgct gttgaagtta aacatgcaga
     2281 taataccctg accttcggtc cgcgtgatgg ttacgcagac ggttgggcac aggctggtac
     2341 cgcgcgtgcc ctgctgaact caatggttat cggtgttacc gaaggcttca ctaagaagct
     2401 gcagctggtt ggtgtaggtt accgtgcagc ggttaaaggc aatgtgatta acctgtctct
     2461 gggtttctct catcctgttg accatcagct gcctgcgggt atcactgctg aatgtccgac
     2521 tcagactgaa atcgtgctga aaggcgctga taagcaggtg atcggccagg ttgcagcgga
     2581 tctgcgcgcc taccgtcgtc ctgagcctta taaaggcaag ggtgttcgtt acgccgacga
     2641 agtcgtgcgt accaaagagg ctaagaagaa gtaaggtaac actatggata agaaatctgc
     2701 tcgtatccgt cgtgcgaccc gcgcacgccg caagctccag gagctgggcg caactcgcct
     2761 ggtggtacat cgtaccccgc gtcacattta cgcacaggta attgcaccga acggttctga
     2821 agttctggta gctgcttcta ctgtagaaaa agctatcgct gaacaactga agtacaccgg
     2881 taacaaagac gcggctgcag ctgtgggtaa agctgtcgct gaacgcgctc tggaaaaagg
     2941 catcaaagat gtatcctttg accgttccgg gttccaatat catggtcgtg tccaggcact
     3001 ggcagatgct gcccgtgaag ctggccttca gttctaaggt agaggtgtaa gatggctcac
     3061 atcgaaaaac aagctggcga actgcaggaa aagctgatcg cggtaaaccg cgtatctaaa
     3121 accgttaaag gtggtcgtat tttctccttc acagctctga ctgtagttgg cgatggtaac
     3181 ggtcgcgttg gttttggtta cggtaaagcg cgtgaagttc cagcagcgat ccagaaagcg
     3241 atggaaaaag cccgtcgcaa tatgattaac gtcgcgctga ataacggcac tctgcaacac
     3301 cctgttaaag gtgttcacac gggttctcgc gtattcatgc agccggcttc cgaaggtacc
     3361 ggtatcatcg ccggtggtgc aatgcgcgcc gttctggaag tcgctggggt tcataacgtt
     3421 ctggctaaag catatggttc caccaacccg atcaacgtgg ttcgtgcaac tattgatggc
     3481 ctggaaaata tgaattctcc agaaatggtc gctgccaagc gtggtaaatc cgttgaagaa
     3541 attctgggga aataaaccat ggcaaagact attaaaatta ctcaaacccg cagtgcaatc
     3601 ggtcgtctgc cgaaacacaa ggcaacgctg cttggcctgg gtctgcgtcg tattggtcac
     3661 accgtagagc gcgaggatac tcctgctatt cgcggtatga tcaacgcggt ttccttcatg
     3721 gttaaagttg aggagtaaga gatgcgttta aatactctgt ctccggccga aggctccaaa
     3781 aaggcgggta aacgcctggg tcgtggtatc ggttctggcc tcggtaaaac cggtggtcgt
     3841 ggtcacaaag gtcagaaatc tcgttctggc ggtggcgtac gtcgcggttt cgagggtggt
     3901 cagatgcctc tgtaccgtcg tctgccgaaa ttcggcttca cttctcgtaa agcagcgatt
     3961 acagccgaag ttcgtctgtc tgacctggct aaagtagaag gcggtgttgt agacctgaac
     4021 acgctgaaag cggctaacat tatcggtatc cagatcgagt tcgcgaaagt gatcctggct
     4081 ggcgaagtaa cgactccggt aactgttcgt ggcctgcgtg ttactaaagg cgctcgtgct
     4141 gctatcgaag ctgctggcgg taaaatcgag gaataagtag cagatggcta aacaaccggg
     4201 attagatttt caaagtgcca aaggtggctt aggcgagctg aaacgcagac tgctgtttgt
     4261 tatcggtgcg ctgattgtgt tccgtattgg ctcttttatt ccgatccctg gtattgatgc
     4321 cgctgtactt gccaaactgc ttgagcaaca gcgaggcacc atcattgaga tgtttaacat
     4381 gttctctggt ggtgctctca gccgtgcttc tatctttgct ctggggatca tgccgtatat
     4441 ttcggcgtcg atcattatcc agctgctgac ggtggttcac ccaacgttgg cagaaattaa
     4501 gaaagaaggg gagtctggtc gtcgtaagat cagccagtac acccgttacg gtactctggt
     4561 gctggcaata ttccagtcga tcggtattgc taccggtctg ccgaatatgc ctggtatgca
     4621 aggcctggtg attaacccag gctttgcatt ctacttcacc gctgttgtaa gtctggtcac
     4681 aggaacgatg ttcctgatgt ggttgggcga acagattact gaacgaggta tcggcaacgg
     4741 tatttcaatc attatcttcg ccggtattgt cgcgggactc ccgccagcca ttgcccatac
     4801 tatcgagcaa gcgcgtcaag gcgacctgca cttcctcgtg ttgctgttgg ttgcagtatt
     4861 agtatttgca gtgacgttct ttgttgtatt tgttgagcgt ggtcaacgcc gcattgtggt
     4921 aaactacgcg aaacgtcagc aaggtcgtcg tgtctatgct gcacagagca cacatttacc
     4981 gctgaaagtg aatatggcgg gggtaatccc ggcaatcttc gcttccagta ttattctgtt
     5041 cccggcgacc atcgcgtcat ggttcggggg cggtactggt tggaactggc tgacaacaat
     5101 ttcgctgtat ttgcagcctg ggcaaccgct ttatgtgtta ctctatgcgt ctgcaatcat
     5161 cttcttctgt ttcttctaca cggcgttggt tttcaacccg cgtgaaacag cagataacct
     5221 gaagaagtcc ggtgcatttg taccaggaat tcgtccggga gagcaaacgg cgaagtatat
     5281 cgataaagta atgacccgcc tgaccctggt tggtgcgctg tatattacct ttatctgcct
     5341 gatcccggag ttcatgcgtg atgcaatgaa agtaccgttc tacttcggtg ggacctcact
     5401 gcttatcgtt gttgtcgtga ttatggactt tatggctcaa gtgcaaactc tgatgatgtc
     5461 cagtcagtat gagtctgcat tgaagaaggc gaacctgaaa ggctacggcc gataattggt
     5521 cgcccgagaa gttacggaga gtaaaaatga aagttcgtgc ttccgtcaag aaattatgcc
     5581 gtaactgcaa aatcgttaag cgtgatggtg tcatccgtgt gatttgcagt gccgagccga
     5641 agcataaaca gcgccaaggc tgattttttc gcatattttt cttgcaaagt tgggttgagc
     5701 tggctagatt agccagccaa tcttttgtat gtctgtgcgt ttccatttga gtatcctgaa
     5761 aacgggcttt tcagcatgga acgtacatat taaatagtag gagtgcatag tggcccgtat
     5821 agcaggcatt aacattcctg atcataagca tgccgtaatc gcattaactt cgatttatgg
     5881 cgtcggcaag acccgttcta aagccatcct ggctgcagcg ggtatcgctg aagatgttaa
     5941 gatcagtgag ctgtctgaag gacaaatcga cacgctgcgt gacgaagttg ccaaatttgt
     6001 cgttgaaggt gatctgcgcc gtgaaatcag catgagcatc aagcgcctga tggatcttgg
     6061 ttgctatcgc ggtttgcgtc atcgtcgtgg tctcccggtt cgcggtcagc gtaccaagac
     6121 caacgcacgt acccgtaagg gtccgcgcaa accgatcaag aaataatcgg ggtgattgaa
     6181 taatggcaaa ggcaccaatt cgtgcacgta aacgtgtaag aaaacaagtc tctgacggcg
     6241 tggctcatat ccatgcttct ttcaacaaca ccatcgtgac tatcactgat cgtcagggta
     6301 acgcgttggg ttgggcaaca gccggtggtt ccggtttccg tggttctcgc aaatccactc
     6361 cgtttgcagc tcaggttgca gcagagcgtt gcgctgacgc cgtgaaagaa tacggcatca
     6421 agaatctgga agttatggtt aaaggtccgg gtccaggccg cgaatctact attcgtgctc
     6481 tgaacgccgc aggtttccgc atcactaata ttactgatgt gactccgatc cctcataacg
     6541 gttgtcgtcc gccgaaaaaa cgtcgcgtat aacgcctcgt tttccaggtt tgttggagaa
     6601 agaaaatggc aagatatttg ggtcctaagc tcaagctgag ccgtcgtgag ggcaccgact
     6661 tattccttaa gtctggcgtt cgcgcgatcg ataccaagtg taaaattgaa caagctcctg
     6721 gccagcacgg tgcgcgtaaa ccgcgtctgt ctgactatgg tgtgcagttg cgtgaaaagc
     6781 aaaaagttcg ccgtatctat ggtgtgctgg agcgtcagtt ccgtaactac tacaaagaag
     6841 cagcacgtct gaaaggcaac accggtgaaa acctgttggc tctgctggaa ggtcgtctgg
     6901 acaacgttgt ataccgtatg ggcttcggtg ccactcgtgc agaagcacgt cagctggtta
     6961 gccataaagc aattatggta aacggtcgtg ttgttaacat cgcttcttat caggttagtc
     7021 cgaatgacgt tgtaagcatt cgtgagaaag cgaagaagca gtctcgcgtg aaagccgctc
     7081 tggagctggc tgagcagcgt gaaaagccaa cctggctgga agttgatgct ggcaagatgg
     7141 aaggtacgtt taagcgtaag ccggagcgtt ctgatctgtc tgcggacatt aacgaacacc
     7201 tgatcgtcga gctttactcc aagtaaagct tagtaccaaa gagaggacac aatgcagggt
     7261 tctgtgacag agtttctaaa accgcgcctg gttgatatcg agcaagtgag ttcgacgcac
     7321 gccaaggtga cccttgagcc tttagagcgt ggctttggcc atactctggg taacgcactg
     7381 cgccgtattc tgctctcatc gatgccgggt tgcgcggtga ccgaggttga gattgatggt
     7441 gtactacatg agtacagcac caaagaaggc gttcaggaag atatcctgga aatcctgctc
     7501 aacctgaaag ggctggcggt gagagttcag ggcaaagatg aagttattct taccttgaat
     7561 aaatctggca ttggccctgt gactgcagcc gatatcaccc acgacggtga tgtcgaaatc
     7621 gtcaagccgc agcacgtgat ctgccacctg accgatgaga acgcgtctat tagcatgcgt
     7681 atcaaagttc agcgcggtcg tggttatgtg ccggcttcta cccgaattca ttcggaagaa
     7741 gatgagcgcc caatcggccg tctgctggtc gacgcatgct acagccctgt ggagcgtatt
     7801 gcctacaatg ttgaagcagc gcgtgtagaa cagcgtaccg acctggacaa gctggtcatc
     7861 gaaatggaaa ccaacggcac aatcgatcct gaagaggcga ttcgtcgtgc ggcaaccatt
     7921 ctggctgaac aactggaagc tttcgttgac ttacgtgatg tacgtcagcc tgaagtgaaa
     7981 gaagagaaac cagagttcga tccgatcctg ctgcgccctg ttgacgatct ggaattgact
     8041 gtccgctctg ctaactgcct taaagcagaa gctatccact atatcggtga tctggtacag
     8101 cgtaccgagg ttgagctcct taaaacgcct aaccttggta aaaaatctct tactgagatt
     8161 aaagacgtgc tggcttcccg tggactgtct ctgggcatgc gcctggaaaa ctggccaccg
     8221 gcaagcatcg ctgacgagta accggatcac aggttaaggt tttactgaga aggataaggt
     8281 catgcgccat cgtaagagtg gtcgtcaact gaaccgcaac agcagccatc gccaggctat
     8341 gttccgcaat atggcaggtt cactggttcg tcatgaaatc atcaagacga ctctgcctaa
     8401 agcgaaagag ctgcgccgcg tagttgagcc gctgattact cttgccaaga ctgatagcgt
     8461 tgctaatcgt cgtctggcat tcgcccgtac tcgtgataac gagatcgtgg caaaactgtt
     8521 taacgaactg ggcccgcgtt tcgcgagccg tgccggtggt tacactcgta ttctgaagtg
     8581 tggcttccgt gcaggcgaca acgcgccgat ggcttacatc gagctggttg atcgttcaga
     8641 gaaagcagaa gctgctgcag agtaatctga agcaacgtaa aaaaacccgc cccggcgggt
     8701 ttttttatac ccgtagcatc cccacttatc tacaatagct gtactctttt tgttcatccc
     8761 ctggagtata tatgtggtta cttgaccagt gggcagagcg tcatatagca gaagcgcaag
     8821 cgaaaggtga gtttgataac ctggcaggta gcggcgaacc attgatactg gatgatgatt
     8881 ctcacgtgcc accggaatta cgtgcggggt atcgcttgtt gaagaatgcc ggttgcttac
     8941 cgccagaact tgagcaacgg agggaagcaa ttcagcttct ggatattctc aaaggtatcc
     9001 gtcacgatga tccgcaatat caagaggtta gccgtcgatt gtcattactg gaattgaagc
     9061 tgcgacaagc tggactgagt accgattttt tacgcggcga ttatgctgac aagttgttga
     9121 ataaaatcaa cgataactag tggagtatgt atgtatcgca ttggtgagct ggcaaaaatg
     9181 gcggaagtaa cacccgacac gattcgttat tacgaaaaac agcagatgat ggagcatgaa
     9241 gtgcgtactg aaggtgggtt tcgcctgtat accgaaagcg atctccagcg attgaaattt
     9301 atccgccatg ccagacaact aggtttcagt ctggagtcga tccgcgagtt gctgtcgatc
     9361 cgtatcgatc ctgaacacca tacctgtcag gagtcaaaag gcattgtgca ggaaagattg
     9421 caggaagtcg aagcacggat agccgagttg cagagtatgc agcgttcctt gcaacgcctt
     9481 aacgatgcct gttgtgggac cgcccatagc agtgtttatt gctcgattct tgaagctctt
     9541 gaacaagggg cgagtggcgt taagagtggc tgttgatttt ttgcactgac aggattacac
     9601 tcgcgccgtt aaataaccaa ctggagtttt tatgagccga tatcagcata ctaaagggca
     9661 gataaaggat aatgcgatag aagcattact acatgatccc ttattccgac agcgagtaga
     9721 gaaaaataag aaagggaaag gcagttacat gcgaaaagga aaacatggca atcgggggaa
     9781 ctgggaggcc agtggcaaga aagtaaatca cttttttacc actggccttc tgctttcagg
     9841 cgtttgttaa gagcggttat tctgctcttt cagcaaatca cgaatttctg tcagtaatac
     9901 ttcttcttta gttggtgctg gtgcggctgc tggttcttct tttttccgat tcagtttgtt
     9961 gattagctta atcgccataa agatggcaaa ggccacaatc agaaaatcaa agacgttttg
    10021 aatgaagaca ccgtaatgca tcacaacagc agggatatcc ccctgcgcat cgcgtagcgt
    10081 gacagcaaac tgtttaaagt cgatcccgcc aattaataag cccagaggtg gcatgatgat
    10141 atcggcaacc agtgaagata caatcttccc gaatgccgca ccgataatga cacccaccgc
    10201 caaatccacc acgttcccgc gcatcgcaaa ttcgcgaaat tctttaataa tgctcatgtt
    10261 attctcccta tgaagccgac aaccataagt ctaacaa
//
LOCUS       DAZGVQ010000171        10272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000171 DAZGVQ010000000
VERSION     DAZGVQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.172"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..1297
                     /gene="dgcT"
                     /locus_tag="ACHLAG_004327"
     CDS             2..1297
                     /gene="dgcT"
                     /locus_tag="ACHLAG_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFP0657451.1"
                     /translation="MVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYF
                     IYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSL
                     CILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFGGPIVAHILSSHYESYNLHIAE
                     LTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRYDIWNGVTVIAFCAVLYNISLL
                     FMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALRVTKNIAHRDPLTNIFNRNYFF
                     NELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLL
                     ARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGDNPEYAIPQKVTISIGAVVTQE
                     NALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP"
     gene            complement(1338..2500)
                     /locus_tag="ACHLAG_004328"
     CDS             complement(join(1338..2239,2239..2500))
                     /locus_tag="ACHLAG_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
                     /protein_id="HFP0657452.1"
                     /translation="MTKTVSTSKKPRKQHSPEFRSEALKLAERIGVTAAARELSLYES
                     QRYNWRSKQQNQQTSSERELEMSTEIARLKRQLAERDEELAILPKGRDILREAPEMKY
                     VFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISTRQQFRQHCDSVVLAAFTRSK
                     QRYGAPRLTDELRAQGYPFNVKTVAASLRRQGLRAKASRKFSPVSYRAHGLPVSENLL
                     EQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDAL
                     QMALWRRKRPRNVIVHTDRGGQYCSADYQAQLKRHNLRGSMSAKGCCYDNACVESFFH
                     SLKVECIHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
     gene            2567..3094
                     /locus_tag="ACHLAG_004329"
                     /pseudo
     CDS             2567..3094
                     /locus_tag="ACHLAG_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            3091..4077
                     /gene="ycdU"
                     /locus_tag="ACHLAG_004330"
     CDS             3091..4077
                     /gene="ycdU"
                     /locus_tag="ACHLAG_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFP0657453.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(4270..4406)
                     /locus_tag="ACHLAG_004331"
     ncRNA           complement(4270..4406)
                     /ncRNA_class="other"
                     /locus_tag="ACHLAG_004331"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(4451..4538)
                     /locus_tag="ACHLAG_004332"
     tRNA            complement(4451..4538)
                     /locus_tag="ACHLAG_004332"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4502..4504),aa:Ser,seq:gga)
     gene            4772..5710
                     /gene="ghrA"
                     /locus_tag="ACHLAG_004333"
     CDS             4772..5710
                     /gene="ghrA"
                     /locus_tag="ACHLAG_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFP0657454.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFSGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5765..6502
                     /gene="ycdX"
                     /locus_tag="ACHLAG_004334"
     CDS             5765..6502
                     /gene="ycdX"
                     /locus_tag="ACHLAG_004334"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFP0657455.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            6526..7080
                     /gene="ycdY"
                     /locus_tag="ACHLAG_004335"
     CDS             6526..7080
                     /gene="ycdY"
                     /locus_tag="ACHLAG_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFP0657456.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            7182..7673
                     /gene="ycdZ"
                     /locus_tag="ACHLAG_004336"
     CDS             7182..7673
                     /gene="ycdZ"
                     /locus_tag="ACHLAG_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFP0657457.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(7737..8570)
                     /gene="csgG"
                     /locus_tag="ACHLAG_004337"
     CDS             complement(7737..8570)
                     /gene="csgG"
                     /locus_tag="ACHLAG_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFP0657458.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(8597..9013)
                     /gene="csgF"
                     /locus_tag="ACHLAG_004338"
     CDS             complement(8597..9013)
                     /gene="csgF"
                     /locus_tag="ACHLAG_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFP0657459.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(9038..9427)
                     /gene="csgE"
                     /locus_tag="ACHLAG_004339"
     CDS             complement(9038..9427)
                     /gene="csgE"
                     /locus_tag="ACHLAG_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFP0657460.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(9432..10082)
                     /gene="csgD"
                     /locus_tag="ACHLAG_004340"
     CDS             complement(9432..10082)
                     /gene="csgD"
                     /locus_tag="ACHLAG_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFP0657461.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
BASE COUNT         2548 a         2293 c         2507 g         2924 t
ORIGIN      
        1 tttggttttg gtgaatagtt ggtttaatca gccaggcgtt gaggaagttg ttccacgatc
       61 aacctatctg atggtaatga ttgctttatt ttttatcgat actgttgcat ttatttttat
      121 gcagttgtat ttcatttatg accgtaggca attttcaaac tgtatactta gtttggcttt
      181 tctgagttgt ttgatttact ttgttaaaac cgtcattatc attcagcaaa ttattgaggg
      241 gcgtttgaca agcagtgttg tccaaaatga cattgcaatc tattatttgt ttcgtcagat
      301 gagtttgtgc atattaatat ttctggcatt ggtgaataaa gttagtgaaa acacaaaaca
      361 gcgcaattta ttttccaaaa aaatgacttt gtgcataagt ttgttttttg tttttggggg
      421 gccgatagtt gctcacatac tttctagtca ttatgagagc tacaatttac acattgccga
      481 gttaaccaat gaaaatggtc aggtggtttg gaaagcctca tatgttacca taatgatttt
      541 catgtggtta accttgcttt cggttaatct atactttaat ggtttacgct atgatatctg
      601 gaatggagtg acggtgattg ctttttgtgc cgtactgtac aatatctctc tgttatttat
      661 gagtaggtat agcgtgtcca cttggtatat tagtcgcact attgaagtag tcagtaagtt
      721 aaccgttatg gtaatattta tgtgccatat attcagtgcg ctacgagtaa caaagaacat
      781 tgcacatcgc gatcccttaa ccaatatatt taacagaaat tattttttta atgaactgac
      841 agttcaatca gcatcagcca aaaaaacgcc ttattgcgtc atgattatgg atatcgacca
      901 cttcaaaaaa gtcaacgaca cctgggggca tccggttggc gatcaggtga taaaaacagt
      961 ggtgaatatc attgggaaaa gcatacgacc agatgacctt ttagcgcgcg tcggcggcga
     1021 agagtttggc gtcttgctga cggacatcga tactgaacgt gcgaaagctt tagcggaaag
     1081 gattcgggaa aatgttgagc gtttaactgg cgataatcct gaatacgcta taccacaaaa
     1141 agtgacgatt agtattggcg ctgttgtgac tcaggaaaat gcgttaaacc cgaatgagat
     1201 ctatcgactg gctgataacg cactttacga ggcgaaagag accgggcgta ataaggtggt
     1261 tgtgagggat gtggtgaatt tttgtgagtc accataaagc ggcattttga tcctacccac
     1321 gtaatatgga cacaggccta agcgaggttc ttgttttcaa attgttccgg actgaggccg
     1381 ccacaccaac tgtgccgccg ccaccgattg taatcacatt cgatataatt aaacaccgtt
     1441 gcccgcatta tttcccggct gataaagtgt tctccatgga tacattccac tttcagcgaa
     1501 tgaaagaagc tttccacgca ggcattatcg tagcagcaac cttttgcgct catacttcca
     1561 cgcagattat gccgcttcag ttgcgcctga taatctgctg aacagtactg gcctccacgg
     1621 tccgtgtgaa cgataacgtt ccggggcctc ttacgccgcc acagcgccat ctgcagggca
     1681 tcgcaggcca gttgcgccgt catgcgtggc gacattgacc agccaataac ggcacgtgac
     1741 cacaggtcaa tgaccactgc cagatacagc cagccttcat ctgtacgtaa gtacgtgatg
     1801 tctcctgccc acttctggtt cgggccactg gcgtaaaaat cctgctccaa cagattttct
     1861 gacacaggca ggccgtgtgc gcggtagctg accgggctga acttccggga ggcctttgcc
     1921 ctcagtccct gacggcgcag gcttgccgcc acggttttta cgttaaaggg gtaaccctga
     1981 gcacgcagtt catccgtcag gcgtggggca ccgtaacgct gttttgaccg ggtaaaagcc
     2041 gcgaggacaa cgctgtcgca gtgttggcgg aactgctgac gcgtgcttat ccttgtccgc
     2101 cgctgacacc acgtatacca gccgctgcgg gccacccgga gcacgcggca cattgctttg
     2161 atgctgaact cagcctgatg tttttcaata aagacatact tcatttcagg cgcttcgcga
     2221 agtatgtcgc ggccttttgg aggatagcca gctcttcatc ccgttctgcc agctggcgtt
     2281 tgagacgtgc aatctcggta gacatctcca gttcacgttc agaagacgtc tgctgatttt
     2341 gctgtttact gcgccagttg tagcgctgtg attcatacag gctgagttca cgggctgcgg
     2401 cagtaacacc gatgcgttca gcaagcttca gggcttcact gcgaaattca ggcgaatgct
     2461 gtttacgggg ttttttactg gttgatactg tttttgtcat gtgagtcacc tctgactgag
     2521 agtttactca cttagccgcg tgtccactat tgctgggtaa gatcaggcgg ttaaaggcat
     2581 caaccgcgaa caactgcaga cgatgattac ggaatcaggc attgcgctgg atattagctg
     2641 tgataacacg ccgcgccagc aggttattgg gggaacgcag gctgcgttga atgaatttgc
     2701 cacgctgctg atggccgctg gatacgaacc ggttaaactc ggtgtgagcg gtgcctggca
     2761 cacgcgtttg atggaagacg gcgttcaggc aatgcgcgat tatcttgccg gactggatat
     2821 cgcttcgcca gagcatcagg ttctgatgaa cgtaacagca aaaagcgagg tcgctccgtc
     2881 aattatcaaa gaaaatctct cattacactt aactcatacg gtgaaatgga ctgaatcttt
     2941 cgatacattt ctgaatatgc caaccccggt agctttcctt gaaataagta ataagcccta
     3001 tttggggaat atgttaaatg attttgctgg cgtggatcag caacgagtta tgcattgtcg
     3061 taaagcattt agtgatgcaa aggtattcaa atgattcctg attatttaac ttttattcgc
     3121 tttcaggata aacgaaatct gatatacatt tatgctattg gacttattct gataggcttt
     3181 tattggaaga atgcagggtt tacttttcca tcagaggata ttggtgtagt tagtgggatt
     3241 ctggccttgg tgctgtataa ttttattttt gatctcaagg cgtactgggc ttataaatgc
     3301 gtcacgaaga atatcgattt ttcgtggttt aagaaaaagc agaaccacaa aatagaatta
     3361 tttcttacac aacctctggt ggcaggattt ctgtcgttaa tcatgttgag tgcaatgagt
     3421 tgggggctat accagcttct accctcgtta tatgcgctgt tcctgatttc gttacttggg
     3481 ccgttggtca tctttctgct gtttcggatg atccgcacca gttatgtcaa gcaggtcgct
     3541 atttcagtag cgaaaaaagt aaaatataaa agtctgactc gctatgtgct gctttcggtg
     3601 tgcatctcaa cggttgttaa cctgcttact atcagcccgt tgcgtaacag tgattctttt
     3661 gtgacagagg ggcagtggtt aacgtttaaa tcgataattg cattgctcat tctttgtggc
     3721 gtagtgttgg cgattaatct gttttttctg cgcttctcca agcggtacgc ttttctgggc
     3781 aggctttttt tgcaggaaat cgatctgttt ttctccagtg aaaatgcgtt gtcgaccttt
     3841 tttgccaagc cgctttggct tcggttattc atattgctgg ttattgaaat gatgtggatt
     3901 acgctggtgt cggtattggc aacgcttgtg gaatggcgga tttggtttga agcctatttt
     3961 ttactctgct atgtaccgtg cttaatttac tattttttcc attgtcgatt cctctggcat
     4021 aacgatttta tgatggcatg tgacatgtat ttccgttggg ggcattttaa taagtgagga
     4081 agttacagga agtgaccaga taacgcatat atgttctcta ctatcttgcg cattttgatt
     4141 gttgactgag taaccagaca gttgatgtgc acaatttccc ctcgccctaa cagacgtggg
     4201 cgggggcacc cggtgtgtgc aatatacgaa aaaaaagccc gtactttcgt acgggctctt
     4261 ctttaaatat ggcggtgagg gggggattga ctcgcttcgc tcgccctgcg ggcagcccgc
     4321 tcactgcgtt cacggtctgt ccaactggct gtcgccagtt gtcgaacccc ggtcggggct
     4381 tctcatcccc ccggtgtgtg caatatacga aaaaaaagcc cgtactttcg tacgagctct
     4441 tctttaaaga tggcggtgag ggggggattc gaacccccga tacgttgccg tatacacact
     4501 ttccaggcgt gctccttcag ccactcggac acctcaccaa attgtcgttc ctgtcttgct
     4561 ggaacgggcg ctaatttagg gaaatcacga cctgaggtca acaaactttt tgaaaaaatc
     4621 gcgcgtttat tcaaacttca atcaatgtgt ggttttaata agcgaaatct gctttttttg
     4681 ccaccgacca cggatttgtt atgctggtgg cctttgtaga tcataacgat aagtgcgaat
     4741 aaatttcgca caacgctttt cgggagtcag tatggatatc atcttttatc acccaacgtt
     4801 cgatacccaa tggtggattg aggcactgcg caaagctatt cctcaggcaa gagtcagagc
     4861 atggaaaagc ggagataatg actctgctga ttatgcttta gtctggcatc ctcctgttga
     4921 aatgctggca gggcgcgatc ttaaagcggt gttcgcactc ggggccggtg ttgattctat
     4981 tttgagcaag ctacaggcac accctgaaat gctgaaccct tctgttccac tttttcgcct
     5041 ggaagatacc ggtatgggcg agcaaatgca ggaatatgct gtcagtcagg tgctgcattg
     5101 gtttcgacgt tttgacgatt atcgcatcca gcaaaatagt tcgcattggc aaccgctgcc
     5161 tgaatatcat cgggaagatt ttaccatcgg cattttgggc gcaggcgtac tgggcagtaa
     5221 agttgctcag agtctgcaaa cctggcgctt tccgctgcgt tgctggagtc gaacccgtaa
     5281 atcgtggcct ggcgtgcaaa gcttttccgg acgggaagaa ctgtctgcat ttctgagcca
     5341 atgtcgggta ttgattaatt tgttaccgaa tacccctgaa accgtcggca ttattaatca
     5401 acaattactc gaaaaattac cggatggcgc gtatctcctc aacctggcgc gtggtgttca
     5461 tgttgtggaa gatgacctgc tcgcggcgct ggatagcggc aaagttaaag gcgcaatgtt
     5521 ggatgttttt aatcgtgaac ccttaccgcc tgaaagtccg ctctggcaac atccacgcgt
     5581 gacgataaca ccacatgtcg ccgcgattac ccgtcccgct gaagctgtgg agtacatttc
     5641 tcgcaccatt gcccagctcg aaaaagggga gagggtctgc gggcaagtcg accgcgcacg
     5701 cggctactaa taaagcatca ggattcctgc tatccttggc gggaattgaa tacaggagag
     5761 agttatgtat cccgtcgacc ttcatatgca taccgttgcc agcacacatg catatagcac
     5821 attgagtgat tacattgccc aggccaaaca aaagggcatt aaactttttg cgatcaccga
     5881 tcatggcccg gatatggaag atgctccgca tcactggcac ttcattaaca tgcgtatctg
     5941 gccgcgagtg gttgatgggg tagggatcct gcgcggcatc gaagctaaca ttaaaaatgt
     6001 tgatggtgaa attgattgca gcggtaaaat gtttgactcg ctggatctga ttattgccgg
     6061 ttttcatgag ccggtttttg caccacatga caaagcgacc aatacacaag cgatgatcgc
     6121 cactatcgcc agcggcaatg tgcatataat aagccatccc ggaaatccca aatatgaaat
     6181 agatgtgaaa gccgttgctg aagcagccgc gaaacatcag gtggcgctgg aaatcaataa
     6241 ttcttcattt ttacactcac gtaagggcag tgaagacaac tgtcgtgcgg tagccgcagc
     6301 ggtacgtgat gctggaggtt gggtggcatt aggctcggat tctcacaccg cgtttaccat
     6361 gggggaattt gaagagtgtc ttaaaatcct cgacgcggta gattttccgc cagagcgcat
     6421 tttgaatgtt tctccgcgcc gcttactgaa cttccttgaa tctcgaggta tggcaccgat
     6481 tgcggaattt gcagaccttt aattactagt aatggaaata tattaatgaa cgagttttct
     6541 atcctctgtc gtgtgctggg ttcgctctat taccgccaac cgcaagatcc tttactggtg
     6601 ccgctgttta ccctgattcg tgaggggaaa ctggctgcga actggccact ggagcaggat
     6661 gagttactga cgcgtttaca gaaaagctgt gatatggcgc aagtctctgc cgattacaat
     6721 gcgttgttta tcggcgatga atgtgccgtg ccgccatatc gtagcgcatg ggttgaggac
     6781 gcaacggaag cggaagtgcg cgcttttctt tccgaacgag ggatgccact ggcggatacg
     6841 ccagccgatc acatcggcac attgctgctt gcggcttcct ggctggaaga tcagtcaacg
     6901 gaagatgaga gcgaagcact ggaaacactg ttcagtgagt atctgttacc ctggtgtggt
     6961 gcgttcctcg gcaaagtgga ggcccatgca accacgcctt tctggcgcac catggcaccg
     7021 ctaacccgtg atgccattag tgcaatgtgg gacgagctgg aagaagattc tgaagagtaa
     7081 atgtgatcat cgtcactcat aactgcaaca gataatttgt tgttgcataa aatgtgtgct
     7141 cgatctcatt catggccgcg ttttctgcta tcatgcgcgg catgaacata cttctctcta
     7201 ttgcaatcac aacaggcatt ctctccggta tctggggatg ggtggctgtt tccctgggct
     7261 tactaagctg ggcgggcttc ctgggctgta cggcctactt tgcctgcccg caaggtgggc
     7321 tgaaagggct ggcgatctcc gctgcaaccc tgcttagtgg cgtggtgtgg gcgatggtca
     7381 ttatttacgg tagtgcactg gcaccacatc tggaaattct cggttatgtc ataaccggta
     7441 ttgtcgcgtt tctgatgtgt attcaggcca aacagctgct gctttcattt gttccgggaa
     7501 cgtttatagg cgcatgcgcg acgtttgccg ggcagggtga ctggaaactg gtgttacctt
     7561 cgctggcgct tgggctggta tttggttacg caatgaaaaa cagcggcctg tggctggcgg
     7621 cgcgtagtgc aaagacggcg caccgtgagc aggaaatcaa aaataaagcg tgaggggcac
     7681 tcacgctttc gcttaaacag taaaatgccg gatgataatt ccggcttttt tatctgtcag
     7741 gattccggtg gaaccgacat atggcggtat ttcaccagaa tgtcgttctg ccgttctgct
     7801 ttgttctgca aatcccacag accacggtcg ataccatcat taatcaggaa aatgacccct
     7861 gtttcgatag ccgacatcag acacagcata acgggttcgt ttgaggtgta acccacttcc
     7921 ccttccagca agcgctggta gtcaataaag cggaaaaccc cggcctgaac ttcataggaa
     7981 agtatcgtct tactggtgtt caccgaggag aggatctcac cggtactcac attgacgaca
     8041 cgcaggttca cggcaatctg atcgagctgg tattgcgtgt ctgcaccgat cccaaaatat
     8101 cttgccccaa caccgcccga tttaacgttg ctttcataac cgataatcga accttcaacc
     8161 ataatatttg ccgccgttaa agattgcagc gggattcggt tattaatagc aaccgtgccg
     8221 ttttcttgtg ccgcacgaat aatcttgcgc tcgttaagca ggttttgtaa gccctggcgc
     8281 tccagcggta taaaccagcg agaatctttc aatgccgtga ccagcattgc cgtggcgctt
     8341 tgcggaacag cagtggagaa gttacttgcc gggtagggtt taaattgccc ggtttcgtcc
     8401 tgaatgttgt ataccgaaac aaagatctta cccgtcggcg ctggcagatg ggtcaaatct
     8461 ttgtagctct gagctcgagg cattaatgtc ggtctggcgg cttctttagg cggggcggtt
     8521 aagcatccgc tcagtaacat gacggcaacc aaaagaaata agcgctgcat gattattttc
     8581 cttatgaagc tggggcttaa aaatcggttg agttattttg taaacccgaa acctggatgg
     8641 tcgaggtttg tccggtttta cgatctgtca cgttcaactg caattgacca tcgcggttag
     8701 caatatcaac aatataatcg ttggtcacca tgcggcccgg tttaccggta ttaatattcg
     8761 acagtagccc acctaaaatt tgtgactgga tggcctgagt aaagttatct aacgctgagg
     8821 gtgtttcaat accaaagtca tcgttatagc tcggatcttt ataagagttt tgggcctgag
     8881 cgctatttaa taaaaaagcg ccattatttg ggttaccacc aaagtttgga ttacggaact
     8941 ggaaagtcat ggttccagcc caacttaatg gcgaaataag catgagtaga actactgcat
     9001 gtttgacacg cattgcagcc tccgaacaat tttttattta gaattcatca tgcgccaaat
     9061 cgcccgtact taataacgcc tgatttatct ggcgacgatt tagtgcttct tcagtttgaa
     9121 tcagtgcaaa gacgacagtt ttctcgaagt ctcttttcaa cggaaataaa aaagtctgga
     9181 aaataacgtc ctgattgacc gttatagtga tccagcttcc ccatcgtgca ctgggccttt
     9241 cattaatcgt taagttaccc gtatagtcac tttcccattt atcactaaag gctcggtaaa
     9301 aatcatggcc aatagatgaa acagtatggt cagttaacaa tcccgggact tctacctcaa
     9361 cggcgtgaag attccctgcg gcgaacagaa attctgccgc cacaatccag cgtaaataac
     9421 gtttcatggc tttatcgcct gaggttatcg tttgcccagg aaaccgcttg tgtccggttt
     9481 tttacggcta tcttcttgaa aagattataa agatgcgttt taaccgtatt ttcgctgatg
     9541 aacaacgaac gagcgatctc gttattagac gcgccgatac gcagcttatt caggatctct
     9601 ttttcccgat gagtaaggag ggctgattcc gtgctgttat aacgatagtt accagaatgt
     9661 gtaatcaggt aactggcaag cttttgcgta aagtagcatt cgccgcgcag gacgccttgc
     9721 aacccattga caacacgttc ttgatcctcc atggcataaa aaacgccgtt gatatgaggc
     9781 cagttttcaa tgtcgcggta cgggtaatct tcaggcgtat ttagcaacaa tattttgata
     9841 ttgttgtttt tcctgctcaa agtatcctgc caataatgga taagcttttt atctgcttcc
     9901 atcatatcca gaagaataat agagcctgaa gatatatcgt ccagagaacg ttgaatatta
     9961 tgtaattttc ctgtaattgc cagcgattgt ttaaggtgct gcaagagagc tgtcgcttgc
    10021 aaagacggtt tagtgatcaa caataatgta tgaccatgaa tactatggac ttcattaaac
    10081 atgatgaaac cccgcttttt ttattgatcg cacacctgac agctgcctct aaaatagaag
    10141 caccagaagt actgacagat gttgcactgc tgtgtgtagt aataaatcag ccctaaatgg
    10201 gtaaaatata aaactaatgg attacatctg atttcaatct agccattaca aatcttaaat
    10261 caagtgttaa ac
//
LOCUS       DAZGVQ010000172        10251 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000172 DAZGVQ010000000
VERSION     DAZGVQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.173"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            89..904
                     /gene="yahL"
                     /locus_tag="ACHLAG_004341"
     CDS             89..904
                     /gene="yahL"
                     /locus_tag="ACHLAG_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFP0657462.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWLDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIHP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            1317..1562
                     /gene="yahM"
                     /locus_tag="ACHLAG_004342"
     CDS             1317..1562
                     /gene="yahM"
                     /locus_tag="ACHLAG_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFP0657463.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRGRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(1579..2250)
                     /locus_tag="ACHLAG_004343"
     CDS             complement(1579..2250)
                     /locus_tag="ACHLAG_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFP0657464.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            2397..2672
                     /gene="yahO"
                     /locus_tag="ACHLAG_004344"
     CDS             2397..2672
                     /gene="yahO"
                     /locus_tag="ACHLAG_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFP0657465.1"
                     /translation="MKIISKMLVGALAFAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            complement(2773..4359)
                     /gene="prpR"
                     /locus_tag="ACHLAG_004345"
     CDS             complement(2773..4359)
                     /gene="prpR"
                     /locus_tag="ACHLAG_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFP0657466.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAVIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAVTVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFMQLLLPELARESAKIPAPRLLTPQQALEKFKGDK
                     TAAANYLGISRTTFWRRLKN"
     gene            4598..5488
                     /gene="prpB"
                     /locus_tag="ACHLAG_004346"
     CDS             4598..5488
                     /gene="prpB"
                     /locus_tag="ACHLAG_004346"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFP0657467.1"
                     /translation="MSLHSPGKAFRAALSKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            5533..6702
                     /gene="prpC"
                     /locus_tag="ACHLAG_004347"
     CDS             5533..6702
                     /gene="prpC"
                     /locus_tag="ACHLAG_004347"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFP0657468.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            6736..8187
                     /gene="prpD"
                     /locus_tag="ACHLAG_004348"
     CDS             6736..8187
                     /gene="prpD"
                     /locus_tag="ACHLAG_004348"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFP0657469.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPTFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRARLEQMPVNEYLDLYVI"
     gene            8227..10113
                     /gene="prpE"
                     /locus_tag="ACHLAG_004349"
     CDS             8227..10113
                     /gene="prpE"
                     /locus_tag="ACHLAG_004349"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFP0657470.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGGV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
BASE COUNT         2521 a         2629 c         2733 g         2368 t
ORIGIN      
        1 acgttagtta tgagcgattt ctgatagtgc ctggtttaat gagagcttta ttatctgcga
       61 cgtttatttt tatttaagag agatcgcgat gatatcatta aaagctccgc acaataattt
      121 gatgccatat acgcaacaaa gcatacttaa tacggttaaa aacaatcagt taccagagga
      181 tatcaaaagc tccctggttt cctgtgtcga tatattcaag gttttgatta aacaatatta
      241 tgattatcct tatgattgtc gtgatgattt agtcgatgac gacaaactca ttcatctcat
      301 ggctgccgta cgagactgtg agtggttaga cgataacgca ctcaccataa atgtgcagtt
      361 taatgatttc cctggatttt atgactggat ggattaccct gatcacccgg ttaagtttgt
      421 ttttcgcata cttgaaaatc aaaaagggac agtttgggta tatgaccagg atgatgcgtt
      481 tcttgatatt aaagcaaatg tgcaggctgg acgctttacc gggcttaaga aattagtgca
      541 gtttatcgat tcagtgcgaa cagattgcaa atgtattctt cttgaacacc atatgccttt
      601 gctgagaatt ttccctaaag ggaaagagtg tatgcgtgtg gaaaaatggc ttcgcgaaat
      661 gtcgtctatt ccggagacag acgcgcccat taagcaggcg cttgcgcatg gattattatt
      721 acacttaaaa aatatccacc ctgtttttcc tgaatcactg gtgatgttat tgctctctgt
      781 tctggacgta aaaacataca gagatgacgc acgattaaat gaatggatat cgaatagagt
      841 gcaggaatta ggtgacagat attatcctgt aaataagcac gttaaaatcc gctacactct
      901 ttagttcaga aagggttcca cctgcaagtt gttattccac cagagagtga atcgcaaagc
      961 aggtaatcat ttcatctgta tatccgtatg ccaaatggca ttccaccgcc tttttcgccg
     1021 atattttgtt gtgatcggcg acgcttcgct taaaaaagca ccagcagtgg ttttgcagct
     1081 atgcggtgta taaaaaatga tctcatgcag atgttttgtg aatgtgtggg ttgacattca
     1141 tatgaaaaaa atcataattc catcatgttt gtgtgggggc ttttctgtac cttacgcatc
     1201 gcactcaagc ctgacagaaa atatgctgta aggctcatat caaaaaccgc cattagctca
     1261 tcaggaagag cagacgacaa ccaggattgt tgtatggtac ggggttcgag gcctcgatgg
     1321 cggtccaact tttcaaaact cttctgaatc agatcccctt attatcatcc ctgcaaagtg
     1381 gaacattgcc attgtttggc tattcaggac ggggcagacc aatgaaaaaa gcccacacag
     1441 gggagagtgg gctgaaatgg gaagctaaag actcaagtaa acttatcgga aataaggacc
     1501 acgcattacg ggggctatca tcgccgatgg ccgggattcg gcaaatcagg ttgattacgt
     1561 agagagtaaa ttatctgctc accgctgcgt caccccttcg taaatcaggc gtagcgcgaa
     1621 tacaccaata attgcaccaa taacccgact ggcaacgcgt tgcatacgcc cataagcacg
     1681 acgcacagcg ggcaaagaaa acgcctgact aagaaaaact cgccagataa ttgatgcgag
     1741 cacaatcccc gcccaggcca ttaaacgtgc ccatgttggt gtttcggcat ttaatgttac
     1801 tgagaaaata ctaataaaaa ataaaacggt ttgcgggtta gagagatcgg taattaatcc
     1861 gcggcgaaaa aagacatacc agggggcgct aatcggttgt tgtagtgtgc tcatttgcgg
     1921 tgttgactgg cggcgcatgc tgcaccacgc aaaccataag agataagcgc cgccgacgat
     1981 tctgataagc gaaaaaatct cctcacactg cgtaattagt gttgcaagac caaacaaacc
     2041 caacccggaa taaaatgcat cgcccagcgc cacccccagc ccggtcagca cacctgcgcg
     2101 tcgaccggaa gccaggctgg tttgtactac cacaaagaga ttggctcccg gattaaaaaa
     2161 agtaatcacg aacagtccta cggtcaggta aacggcgtgc aaaggatcca tagtgatttc
     2221 atccataaat aagtgaagta acttcatcat atttctacca aaaataatcg ggtgcgaaag
     2281 agatcacaaa gtgtcttatt tccggttact ggcgtttatg ccctgactga actaattatt
     2341 aatcaaccca ataatgtggg tgggtgatag tgtgataaca actctggagc cgtaatatga
     2401 aaataatctc taaaatgtta gtcggtgcgt tagcgtttgc cgttaccaat gtctatgccg
     2461 ctgaattgat gaccaaagcg gaatttgaaa aagttgaatc gcagtatgaa aaaataggtg
     2521 atatttcaac cagtaatgaa atgtcgaccg cagatgcgaa agaggatttg atcaaaaaag
     2581 cggatgaaaa aggggctgat gtgttggtac tgacctctgg tcaaactgac aataagatcc
     2641 acggcacggc aaatatttat aagaagaagt aattctgaaa cctatgtaaa catccccgat
     2701 gcgtaaattt atcggtgatc atcatctatt gaaatttatg ccggataaag cgttcgcgct
     2761 gcattcggca gttcagtttt tcagccgccg ccagaacgtc gtccggctta tgcctaaata
     2821 attcgccgct gctgttttat cgcctttaaa tttctccagt gcctgttgtg gtgtcagtaa
     2881 gcgtggagcg ggaattttcg ccgactcgcg cgccagctcc ggcagtagca gttgcataaa
     2941 ctgcggcgtt aaatccggcg tcggttccac acttaaaaac agcgccagtc gttccatcat
     3001 attgcgcagt tcacgaatat tgcctggcca gtcgtagtgc accagcacgg tttcgcttgc
     3061 ctgtaatccc tggcgtaatg cggcagaaaa cggggcggag agcgccgcca gagacacttt
     3121 caaaaagctt tccgccagcg gaagaatatc cgccacccgc tcgcgcagtg gtggcaattg
     3181 cagacgcaaa atactcagcc gataaaacag atcacggcga aactgccctt gccgcatatc
     3241 ttcttccaga ttgcagtgag tggcgctaat gacccgcaca tccaccggaa caggctgatg
     3301 cccgccgacg cgagtaacct ctttttcttc cagcacccgt aacaggcgag tctgcaaagg
     3361 tagcggcatt tcgccaatct catccagaaa cagcgtaccg ccgtgggcaa tttcgaacag
     3421 cccggcgcga cctccgcgtc gcgagccggt aaacgcccct tcctcatagc caaacagctc
     3481 cgcttccagc agcgattcgg ctatcgcccc gcagttgacg gcaacaaacg gatgcgactt
     3541 tttgccctgt cgcgcatcgt ggcgggcaaa atattcccga tgaatcgcct gggccgccag
     3601 ctctttgccc gtccccgttt ccccttcaat caacaccgcc gcactggagc gggcatacag
     3661 caaaatagtc tgccgtactt gttccatctg tggtgattga ccgagcatat cgcccagcac
     3721 gtaacgagtt ctcagggcgt tgcgggtggc atcgtgagtg ttatggcgta acgacatgcg
     3781 cgtcatatcc agcgcatcgc tgaacgcctg gcgcacggtg acggcggaat agataaaaat
     3841 tccggtcatt ccggcttctt ctgccagatc ggtaatcagc cccgcaccga ccaccgcttc
     3901 ggtgccgtta gctttgagct cgttaatctg cccgcgggcg tcttcttcgg taatgtagct
     3961 acgttgatcg aggcgcaaat taaaggtttt ttgaaacgcc accagtgccg gaatagtttc
     4021 ctgataagtg acaacgccga tagaagaggt gagctttccg gcttttgcca gtgcctgtaa
     4081 cacatcgtag ccgctcggtt taatcaaaat aactggcact gacaggcggc ttttcaggta
     4141 cgcgccgtta gagccagcgg cgatgacggc gtcgcagcgt tcgtttgcca gtttcttgcg
     4201 gatataggtc actgcttttt caaagccaag ctggataggg gtaatgttcg ccaggtgatc
     4261 aaactcgagg ctgatatcgc gaaacagctc gaacaggcgc gttacagata ccgtccagat
     4321 aaccggtttg tcgtcattaa gccgtggtgg atgtgccata gcgcaccgca aagttaagaa
     4381 accgaatatt gggtttagtc ttgtttcata attgttgcaa tgaaacgcgg tgaaacattg
     4441 cctgaaacgt taactgaaac gcatatttgc ggattagttc atgactttat ttataacaaa
     4501 ttgaaattaa acatttaatt ttattcagtt aattgtggca caccccttgc tttgtcttta
     4561 tcaacgcaag taacaagttg ataacaaagg atgggctatg tctctacact ctccaggtaa
     4621 agcgtttcgc gctgcactgt ctaaagaaaa tccattgcag attgttggca ccatcaacgc
     4681 taatcatgcg ctgttggcgc agcgtgccgg atatcaggca atttatctct ccggcggtgg
     4741 cgtggcggca ggttcgctgg ggctgcccga tctcggtatt tctacccttg atgatgtgct
     4801 gaccgacatt cgccgtatca ccgacgtttg ttcgctgccg ctgctggtgg atgcggatat
     4861 cggttttggt tcttcggcct ttaacgtggc gcgtacggtg aaatcgatga ttaaagccgg
     4921 tgcggcagga ttgcatattg aagatcaggt tggtgcgaaa cgctgcggtc atcgcccgaa
     4981 caaagcgatc gtctcgaaag aggagatggt ggatcggatc cgcgcggcgg tggatgcgaa
     5041 aaccgatcct gattttgtga tcatggcgcg caccgatgcg ctggcggtag aggggctgga
     5101 tgcggcgatc gagcgtgcac aggcctatgt tgaagcgggt gccgagatgt tgttcccgga
     5161 ggcgattacc gaactcgcca tgtaccgcca gtttgccgat gcggttcagg tgccaatcct
     5221 cgccaacatt accgaatttg gtgccacgcc gctgtttacc accgacgaat tacgcagcgc
     5281 ccatgtcgca atggcgctgt acccgctttc agcgttccgc gccatgaacc gcgccgctga
     5341 acatgtctac aacgtcctgc gccaggaagg cacgcagaaa agcgtcatcg acaccatgca
     5401 gacccgcaac gaactgtacg aaagcatcaa ctactaccag tacgaagaga agctcgacga
     5461 cctgtttgcc cgtagccagg tgaaataaaa cacaacgaaa aatatctcga ccctacaaat
     5521 gacgaggaca atatgagcga cacaacgatc ctgcaaaaca gtacccatgt cattaaaccg
     5581 aaaaaatcgg tggcactttc cggcgttccg gcgggcaata cggcgctctg caccgtgggt
     5641 aaaagtggca atgacctgca ttaccgtggc tacgatattc ttgatctggc ggaacattgc
     5701 gaatttgaag aagtggctca tctgctgatc cacggcaaac tgccgacccg tgacgaactc
     5761 gccgcttaca aaacgaaact gaaagccctg cgtggtttac cggctaacgt gcgtaccgtg
     5821 ctggaagcct taccggcggc gtcacacccg atggatgtta tgcgcaccgg tgtttccgcg
     5881 ctcggctgca cgctgccaga aaaagagggg cataccgtct ctggcgcgcg ggatattgcc
     5941 gacaaactgc tggcgtcgct tagctcgatt ctcctttatt ggtatcacta cagccacaac
     6001 ggcgaacgca tccaaccgga aaccgatgac gactccatcg gcggtcactt cctgcatctg
     6061 ctgcacggcg aaaagccatc gcaaagctgg gaaaaggcga tgcatatctc gctggtgctg
     6121 tacgccgaac acgagtttaa cgcctccacc tttaccagtc gggtgattgc gggcaccggc
     6181 tctgatatgt attccgcgat tattggcgcg attggcgcac tgcgcgggcc aaaacacggc
     6241 ggggcgaatg aagtgtcgct ggagatccag caacgctacg aaacgccgga cgaagccgaa
     6301 gcagatatcc gcaagcgcgt ggaaaacaaa gaagtggtca ttggttttgg tcatccggtt
     6361 tacaccatcg ctgacccgcg ccaccaggtg attaaacgtg tggcgaagca gctctcgcag
     6421 gaaggcggct cgctgaagat gtacaacatc gccgatcgcc tggaaacggt gatgtgggag
     6481 agcaaaaaga tgttccccaa tctcgactgg ttctctgctg tttcctacaa catgatgggc
     6541 gttcccaccg agatgttcac accactgttt gttatcgccc gcgtcaccgg ctgggcggcg
     6601 cacattatcg aacaacgtca ggacaacaaa attatccgtc cttccgccaa ttatgttgga
     6661 ccggaagacc gccagtttgt cgcgctggat aagcgccagt aaacctctac gaataacaat
     6721 aaggaaacgt acccaatgtc agctcaaatc aacaacatcc gcccggaatt tgatcgtgaa
     6781 atcgttgata tcgtcgatta cgtcatgaac tacgaaatca gctccaaagt agcctacgac
     6841 accgcacatt actgcctgct cgacacgctc ggctgcggtc ttgaagctct cgaataccca
     6901 gcctgtaaaa aactgctggg gccaattgtc cccggcaccg tcgtacctaa cggcgtgcgc
     6961 gtccccggaa ctcagttcca gctcgacccc gtccaggcgg catttaacat cggcgcgatg
     7021 atccgttggc tcgatttcaa cgatacctgg ctggcggcgg agtgggggca tccttccgac
     7081 aacctcggcg gcattctggc aacggcagac tggctttcgc gcaacgcggt cgccagcggc
     7141 aaagcgccgt tgaccatgaa acaggtgctg accggaatga tcaaagccca tgaaattcag
     7201 ggctgcatcg cgctggaaaa ctcctttaac cgcgttggcc tcgaccacgt tctgttagtg
     7261 aaagtggctt ccaccgccgt ggtcgccgaa atgcttggcc tgacccgcga ggaaattctc
     7321 aacgccgttt cgctggcgtg ggtggacggt cagtcgctgc gcacctatcg ccatgcgccg
     7381 aacaccggca cgcgtaaatc ctgggcggcg ggcgatgcca cttcccgcgc ggtacgtctg
     7441 gcactgatgg cgaaaacggg cgaaatgggt tacccgtcag ccctgactgc gccggtgtgg
     7501 ggcttctacg acgtctcctt caaaggtgaa tcgttccgct tccagcgccc gtacggttct
     7561 tacgtcatgg aaaatgtgct gttcaaaatc tccttcccgg cggagttcca ctcccagacg
     7621 gcagttgaag cggcgatgac gctctatgaa cagatgcaag cagcaggcaa aacggcggcg
     7681 gatatcgaaa aagtgaccat tcgcacccac gaagcctgta ttcgcatcat cgacaaaaaa
     7741 gggccgctca ataacccggc tgaccgcgac cactgcattc aatacatggt ggcgatcccg
     7801 ctgttgttcg ggcgcttaac ggcggcagat tacgaggaca acgttgcgca agataaacgc
     7861 attgacgccc tgcgcgagaa gatcaattgc tttgaagatc cgacgtttac cgctgactac
     7921 cacgacccgg aaaaacgcgc catcgccaat gccattactc ttgagttcac cgacggcacg
     7981 cgctttgaag aagtggtggt ggagtacccc attggtcatg ctcgccgccg tcaggatggc
     8041 attccgaagc tggtggataa attcaaaatc aatctcgcgc gccagttccc gactcgccag
     8101 cagcagcgca ttctggaggt ttctctcgac agagctcgcc tggaacagat gccggtcaat
     8161 gagtatctcg acctgtacgt catttaagta aacggcggta aggcgtaagt tcaacaggag
     8221 agcattatgt cttttagcga attttatcag cgttcgatta acgaaccgga gcagttctgg
     8281 gccgagcagg cccggcgtat tgactggcag acgcccttta cgcaaacgct cgatcacagc
     8341 aatccgccgt ttgcccgttg gttttgtgaa ggccgaacca acttgtgtca caacgctatc
     8401 gatcgctggc tggagaaaca gccagaggcg ctggcgctga ttgccgtctc ttcggaaaca
     8461 gaagaagagc gcacctttac ctttcgccag cttcatgacg aagtaaacgc ggtggcgtca
     8521 atgctgcgct cgctgggcgt gcagcgtggc gatcgggtgc tggtgtatat gccgatgatt
     8581 gccgaagcgc atattaccct gctggcctgc gcgcgcattg gcgctattca ctcggtggtg
     8641 tttggtggat ttgcctcgca cagcgtggcg gcgcgaattg atgatgctaa accggtgctg
     8701 attgtctcgg ctgatgccgg agcgcgcggt ggcaaaatca ttccctataa aaaattgctc
     8761 gacgatgcga taagtcaggc gcagcaccag ccgcgccatg ttttgctggt ggatcgcggg
     8821 ctggcgaaaa tggcgcgcgt cagcgggcgg gatgtcgatt tcgcgtcgtt gcgccatcaa
     8881 cacatcggcg cgcgggtacc ggtggcgtgg ctggaatcca acgaaacctc ctgcattctc
     8941 tacacctccg gcacgaccgg caaacctaaa ggcgtgcagc gtgacgtcgg cggatatgcg
     9001 gtggcgctgg cgacctcgat ggacaccatt tttggcggca aagcgggcgg tgtgttcttt
     9061 tgtgcttcgg atatcggctg ggtggtgggg cattcgtata tcgtctacgc gccgctgctg
     9121 gcggggatgg cgactatcgt ttacgaagga ttaccgacct ggccggactg cggcgtgtgg
     9181 tggaaaatcg tcgagaaata tcaggttagc cggatgttct cagcgccgac cgccattcgc
     9241 gtgctgaaaa aattccctac cgctgaaatt cgcaaacacg atctctcgtc gctggaagtg
     9301 ctctatctgg ctggagaacc gctggacgag ccgaccgcca gttgggtgag caatacgctg
     9361 gatgtgccgg tcatcgacaa ctactggcag accgaatccg gctggccgat tatggcgatt
     9421 gctcgcggtc tggacgacag gccgacgcgt ctgggaagcc ccggcgtgcc gatgtatggc
     9481 tataacgtgc agttgcttaa tgaagtcacc ggcgaaccgt gtggcgtcaa cgagaaaggg
     9541 atgctggtgg tggaagggcc gctgccgccg gggtgtattc agaccatctg gggcgatgac
     9601 gaccgctttg tgaagacgta ctggtcgctg ttttcccgtc cggtgtacgc cacctttgac
     9661 tggggcatcc gcgatgctga cggttatcac tttattctcg ggcgcactga cgatgtgatt
     9721 aacgttgccg ggcatcggct gggcacgcgc gagattgaag agagtatttc cagtcatccg
     9781 ggcgttgccg aagtggcggt ggttggggtg aaagatgcgc tgaaagggca ggtggcggta
     9841 gcgtttgtca ttccgaaaga gagcgacagt ctggaagatc gtgatgtggc gcattcgcaa
     9901 gagaaggcga ttatggcgct ggtggacagc cagattggca actttggccg cccggcgcac
     9961 gtctggtttg tctcgcaatt gccaaaaacg cgatccggaa aaatgctgcg ccgcacgatc
    10021 caggcgattt gcgaaggacg cgatcctgga gatctgacga ccattgatga tccagcgtcg
    10081 ttggatcaga tccgccaggc gatggaagag taaggtattg tcggatgcgt cgcgtggcgc
    10141 atccgacacc atgcgccgat gcctgatgcg acgcttaccg cgtcttatta tgcctacagg
    10201 cctgaaccgt aggtcggata aggcgctcgc gtcgcatccg acaccccgcg c
//
LOCUS       DAZGVQ010000173        10242 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000173 DAZGVQ010000000
VERSION     DAZGVQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10242
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.174"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1964
                     /gene="acnA"
                     /locus_tag="ACHLAG_004350"
     CDS             <1..1964
                     /gene="acnA"
                     /locus_tag="ACHLAG_004350"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFP0657471.1"
                     /translation="GQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVG
                     KFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELV
                     EKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNE
                     LEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVT
                     LGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDP
                     IETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASEP
                     IGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDT
                     YGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSP
                     AGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSD
                     VVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRS
                     NLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVP
                     CRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(2028..2618)
                     /gene="ribA"
                     /locus_tag="ACHLAG_004351"
     CDS             complement(2028..2618)
                     /gene="ribA"
                     /locus_tag="ACHLAG_004351"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFP0657472.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            2788..3552
                     /gene="pgpB"
                     /locus_tag="ACHLAG_004352"
     CDS             2788..3552
                     /gene="pgpB"
                     /locus_tag="ACHLAG_004352"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFP0657473.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            3701..4009
                     /gene="lapA"
                     /locus_tag="ACHLAG_004353"
     CDS             3701..4009
                     /gene="lapA"
                     /locus_tag="ACHLAG_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFP0657474.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            4016..5185
                     /gene="lapB"
                     /locus_tag="ACHLAG_004354"
     CDS             4016..5185
                     /gene="lapB"
                     /locus_tag="ACHLAG_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFP0657475.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            5378..6115
                     /gene="pyrF"
                     /locus_tag="ACHLAG_004355"
     CDS             5378..6115
                     /gene="pyrF"
                     /locus_tag="ACHLAG_004355"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFP0657476.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            6115..6441
                     /gene="yciH"
                     /locus_tag="ACHLAG_004356"
     CDS             6115..6441
                     /gene="yciH"
                     /locus_tag="ACHLAG_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFP0657477.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(6567..6785)
                     /gene="osmB"
                     /locus_tag="ACHLAG_004357"
     CDS             complement(6567..6785)
                     /gene="osmB"
                     /locus_tag="ACHLAG_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFP0657478.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(7054..7803)
                     /gene="yciT"
                     /locus_tag="ACHLAG_004358"
     CDS             complement(7054..7803)
                     /gene="yciT"
                     /locus_tag="ACHLAG_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFP0657479.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(7893..8066)
                     /gene="yciZ"
                     /locus_tag="ACHLAG_004359"
     CDS             complement(7893..8066)
                     /gene="yciZ"
                     /locus_tag="ACHLAG_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFP0657480.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            8103..8231
                     /gene="ymiD"
                     /locus_tag="ACHLAG_004360"
     CDS             8103..8231
                     /gene="ymiD"
                     /locus_tag="ACHLAG_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFP0657481.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            complement(8213..10198)
                     /gene="pdeR"
                     /locus_tag="ACHLAG_004361"
     CDS             complement(8213..10198)
                     /gene="pdeR"
                     /locus_tag="ACHLAG_004361"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP0657482.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSFTGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
BASE COUNT         2598 a         2426 c         2616 g         2602 t
ORIGIN      
        1 taggccagcc ggtttccatg cttatcccgg acgtagtggg ctttaaactt accggtaaat
       61 tacgtgaagg tattaccgcc acagacctgg ttctcactgt tacccaaatg ctgcgcaaac
      121 atggcgtggt ggggaaattc gtcgaatttt atggtgatgg tctggattca ctaccgctgg
      181 cggatcgcgc caccattgcc aatatgtcgc cagaatatgg tgccacctgt ggcttcttcc
      241 caatcgatgc tgtaaccctc gattacatgc gtttaagcgg gcgcagcgaa gatcaggttg
      301 agttggtcga aaaatatgcc aaagcgcagg gcatgtggcg taacccgggc gatgaaccga
      361 tttttaccag tacgttagaa ctggatatga atgacgttga agcgagcctg gcagggccaa
      421 aacgcccaca ggatcgcgtt gcactgcccg atgtaccaaa agcatttgcc gccagtaacg
      481 aactggaagt gaatgccacg cataaagatc gccagccggt cgattatgtt atgaacggac
      541 atcagtatca gttacctgat ggcgctgtgg tcattgctgc gataacctcg tgcaccaaca
      601 cctctaaccc aagtgtgctg atggctgcag gcttgctggc gaaaaaagcc gtaactcttg
      661 gcctcaagcg gcaaccgtgg gtcaaagcgt cgctggcacc aggctcgaaa gttgtttctg
      721 attatctggc aaaagcgaaa ttgacaccgt atctcgacga actggggttt aaccttgtgg
      781 gatacggttg taccacctgt attggtaact ctgggccgct gcccgatcct atcgaaacgg
      841 caatcaaaaa aggcgattta accgtcggtg cagtgctgtc cggcaaccgt aactttgaag
      901 gccgtatcca tccgctggtt aaaaccaact ggctggcctc gccgccgctg gtggttgcct
      961 atgcgctggc gggaaatatg aatatcaacc tggcttctga gcctatcggc catgatcgta
     1021 aaggcgatcc ggtatatctg aaagatatct ggccatcggc acaagaaatt gcccgtgcgg
     1081 tagaacaagt ctccacagaa atgttccgca aagagtacgc agaagttttt gaaggcacag
     1141 cagagtggaa ggaaattaac gtcacacgat ccgataccta cggttggcag gaggactcaa
     1201 cctatattcg cttatcgcct ttctttgatg aaatgcaggc aacaccagca ccagtggaag
     1261 atattcacgg tgcgcggatc ctcgcaatgc tgggggattc agtcaccact gaccatatct
     1321 ctccggcggg cagtattaag cccgacagcc cagcgggtcg atatctacaa ggtcggggtg
     1381 ttgagcgaaa agactttaac tcctacggtt cgcggcgtgg taaccatgaa gtgatgatgc
     1441 gcggcacctt cgccaatatt cgcatccgta atgaaatggt gcctggcgtt gaagggggga
     1501 tgacgcggca tttacctgac agcgacgtag tctctattta tgatgctgcg atgcgctata
     1561 agcaggaaca aacgccgctg gcggtgattg ccgggaaaga gtatggatca ggctcaagcc
     1621 gtgactgggc ggcaaaaggt cctcgtctgc ttggaattcg tgtggtgatt gccgaatcgt
     1681 ttgaacgaat tcaccgttcg aatttaattg gcatgggcat cctgccgctg gaatttccgc
     1741 aaggcgtaac gcgtaaaacg ttaggactaa ccggggaaga gaagattgat attggcgatc
     1801 tgcaaaatct acaacccggt gcgacggttc cggtgacgct tacgcgcgcg gatggtagcc
     1861 aggaggtcgt accctgccgt tgtcgtatcg acaccgcgac ggagttgacc tactaccaga
     1921 acgacggcat tttgcattat gtcattcgta atatgttgaa gtaacaagta tttgcttgcc
     1981 ggttattttg cttccggcaa gcaaactaat tacacaatgc aagagggtta tttgttcagc
     2041 aaatggccca ttttctcggc tttggtatcg agataatgtt cgttattggg gttacgacct
     2101 acaatcaatg gtacgcgttc aacaatatta atccctgctt cggtcagaat ttcgactttt
     2161 ttcgggttat tggttaacaa gcggacttca ttgacgccaa ggagtttgaa catatcagcg
     2221 caaagagtga agtcgcgctc atcagcggcg aagcctaact ggtggttagc ctctacggta
     2281 tcgtaacctt gatcctgcag tgcgtaagcg cggattttat tcagcagacc aatgttacga
     2341 ccttcctgac ggtgatacag caaaatacca cggccttcct cggcaatttg cgtcaatgcc
     2401 gcttcgagct ggaagccaca atcgcagcgc aagctgaaca gggcgtcacc ggtcagacat
     2461 tcggaatgga cgcgcgcaag taccggggta tgcccggaaa tatcgccata gactagcgcg
     2521 acatgatcgt gtccggttgc cagttcttca aatcccacca tcaggaaatc gccccatggg
     2581 gttggcagtt tggcttctgc cacacgttta agctgcatga aattctccag ataatgctgg
     2641 ttctgtattg gcttattttg ccataacgag aaggggttca cctaatcacg acgcgtcgat
     2701 cgttcacgga atggcacaaa tctgtcaatt tttcctggaa ctggcgtttt cagttatgat
     2761 tgtgggactt atcaaaaagg agaggccatg cgttcgattg ccagacgtac cgcagtggga
     2821 gctgcactat tgcttgtcat gccagtagcc gtatggattt ctggctggcg ttggcaacct
     2881 ggagaacaaa gttggctact aaaagcggct ttttgggtta ctgaaactgt cacccagccc
     2941 tggggcgtca ttacacattt gattttattc ggctggtttc tctggtgtct gcgttttcgc
     3001 attaaggctg cctttgtatt atttgccatt ctggcggccg caatccttgt gggacaaggc
     3061 gttaaatcct ggatcaaaga caaagtccag gaaccacgac cttttgttat ctggctggaa
     3121 aaaacacatc atattccggt tgatgagttc tacactttaa agcgagcaga acgcggaaat
     3181 ttagtgaaag aacagttggc tgaagagaaa aatatcccac aatatttgcg ttcacactgg
     3241 cagaaagaga cggggtttgc ctttccttcc ggtcacacga tgtttgctgc cagttgggca
     3301 ctgctggccg ttggtttgct gtggccgcgt cggcgaacgt taaccattgc tatcttgctg
     3361 gtctgggcaa cgggagtcat gggaagccgc ctgctgctcg ggatgcattg gccacgcgat
     3421 ctggtagtag ctacgttgat ttcgtgggcg ctggtggcgg tggcaacgtg gcttgcgcaa
     3481 cgaatttgtg ggccattaac accacctgcg gaagaaaatc gcgaaatagc gcaacgagaa
     3541 caagaaagtt aacgctggtt gattttccga atttagccct taaatcatca acaatgcgcg
     3601 tggatgccat ttcgcagacg gcgcgaaaat ggtactttaa agggctattg cggtaagttg
     3661 accataattt attcgctcta accacataac gggaagtaat gtgaaatatt tactcatttt
     3721 cttactggtg ttagcgatct tcgtgatttc ggtcacgttg ggtgcgcaga acgatcaaca
     3781 ggtgacgttt aattatctgt tagcgcaagg ggagtaccgt atttccacat tgttggcggt
     3841 attgtttgct gcggggtttg ctatcggttg gttgatttgt ggcctgttct ggctgcgagt
     3901 tcgtgtttcc ctggcgcgcg ctgaacgtaa aataaagcga ctggaaaacc agctttcacc
     3961 cgcgactgac gtggctgtag tgccgcactc gtcagcggcg aaggaataac tttctatgct
     4021 ggagttgttg tttctgcttt tgcctgtagc cgctgcctat ggctggtata tgggccgcag
     4081 aagtgcgcaa caaaacaagc aagatgaagc caaccgcttg tcgcgtgatt acgtagcggg
     4141 ggttaacttc ctgcttagta atcaacagga taaagcggta gacctgtttc tcgatatgct
     4201 taaagaggat acaggcaccg ttgaagccca ccttacgctc ggaaacctgt tccgttcgcg
     4261 tggcgaagtt gatcgcgcta ttcgcatcca tcagacccta atggaaagcg cctcactgac
     4321 ctatgaacag cgtctgttgg cgattcaaca actggggcgt gattacatgg ccgccgggtt
     4381 atacgaccgc gcggaagaca tgttcaatca gctgaccgat gaaactgact tccgcattgg
     4441 cgcgctgcaa cagttgctac aaatctacca ggctaccagc gagtggcaga aagcaattga
     4501 tgttgccgaa cgcctggtga agctgggtaa agataaacag cgcgtcgaaa ttgcccattt
     4561 ctactgtgag ttagccctgc agcatatggc cagcgacgat ctcgatcgtg ccatgacctt
     4621 gctaaaaaaa ggggcggcgg cagataaaaa cagcgcccgc gtatccatca tgatgggacg
     4681 cgtgtttatg gcgaaaggag aatacgccaa agccgtcgaa agtttgcaac gcgtgatatc
     4741 ccaggacaga gaactggtca gcgaaacgct ggaaatgttg caaacctgct atcagcagtt
     4801 gggtaaaact gccgaatggg cagaattcct gcaacgcgcg gtggaagaga acaccggtgc
     4861 cgatgctgaa ttgatgcttg ctgatatcat cgaagcgcgc gacggtagtg aggccgcaca
     4921 ggtctatatt acgcgccagc ttcagcgtca tccgaccatg cgtgtgttcc ataagttaat
     4981 ggattaccac ttaaatgaag cggaagaagg gcgtgccaaa gagagcctga tggtgctgcg
     5041 tgacatggtt ggcgaaaagg tacgtagtaa gcctcgttat cgctgccaga aatgtggttt
     5101 taccgcatac accctctact ggcattgtcc atcttgtcgg gcctggtcaa ccattaaacc
     5161 gattcgcggt cttgatggcc tgtaattttt aaaaaaatcc gactttagtt acaacatact
     5221 agttattgat gttccattgt gctccggcaa cgacggagca gaaaaagcct gccaggggaa
     5281 aaatcgcaac tgttaatttt ttatttccac gggtagaatg ctcgccgttt acctgtttcg
     5341 cgccacttcc ggtgcccatc atcaagaagg tctggtcatg acgttaactg cttcatcttc
     5401 ttcccgcgct gttacgaatt ctcctgtggt tgttgccctt gattatcata atcgtgatga
     5461 cgcgctgtcc tttgtcgaca agatcgaccc acgcgattgt cgtctgaagg tcggcaaaga
     5521 gatgtttaca ttgtttgggc cacagtttgt gcgcgaactt caacagcgtg gttttgatat
     5581 ctttcttgac ctgaaattcc acgatattcc taacactgca gcgcacgctg tcgctgctgc
     5641 agctgactta ggcgtgtgga tggtgaatgt tcatgcctct ggtggggcgc gtatgatgac
     5701 cgcagcgcgt gaggcactgg ttccgtttgg caaagatgca ccgcttttga ttgctgtgac
     5761 agtgttgacc agcatggaag ctagcgacct ggccgatctt ggcgtgacac tgtcacctgc
     5821 agattatgca gaacgtctgg cggcactgac gcaaaaatgt ggccttgatg gtgtggtgtg
     5881 ttctgctcag gaagctgtgc gctttaaaca ggtattcggt caggagttca aactggttac
     5941 accgggcatt cgtccgcagg ggagtgatgc tggtgaccag cgccgcatta tgacgccaga
     6001 acaggcgttg gcggctggtg ttgattatat ggtgattggt cgcccggtaa cgcaatcggt
     6061 agatccagcg cagacgctga aagcgatcaa cgcctcttta cagcggagtg catgatgagt
     6121 gattccaaca gccgtctggt ctactcaacg gaaaccggac gtattgatga gcccaaagcg
     6181 gcccctgtac gccctaaagg cgacggtgtg gtgcgtattc agcgtcagac cagtggacgt
     6241 aaaggtaagg gcgtttgcct gattaccggt gtcgatctcg atgatgccga actgacaaaa
     6301 cttgcagcgg aactgaagaa aaaatgcggc tgcggcggag cagttaaaga tggagttatt
     6361 gaaatccagg gcgataagcg tgatttatta aaatcactac ttgaagcgaa agggatgaag
     6421 gtaaaactcg caggcggtta acataaaaag ccacggatat atccgtggct ttcgaatatt
     6481 ttactgtgcg tattattcag ttatttcgta ctgaacagaa tcaagcgtat tataattgcg
     6541 agtcgataat cgacatttat ttacgattat ttaccgacct ggtgaccaat aacaccaccg
     6601 acagctgcac cacctaatgt acccaacgta ctgccatcgg tcagtactgc accgcctaat
     6661 gcccctgcac ccgcgccgat tgcggtgttg cggtcccgtt tagaccagtt agaacaggca
     6721 ctcagagaca ttgccaaagt aattgccaga acagccgcgg tcattttttt gctcgttaca
     6781 aacataatac tctctcctga atttatgatt cacggaagta agctctctat aactataata
     6841 gctaagaata agcctggtga aattattccg tgaaatctgc tcgcgcgcag ggttatatca
     6901 tgcaggtgat aatgacttcc tgttatatcg ctgataataa ttttatatct taagcgtgtt
     6961 aataacaggt aaatagtctt aattatcaac taggaatcat cttagagcgg atgatttgcc
     7021 aaactgcaaa tcatccgtag agaagggaaa tggttaaatg tcaacgacgt gaacggtgag
     7081 tttactgtgc tcaagatgca tcagatcgct ggcgcgtatt ttcgaatcgg taatcacgcg
     7141 attgaatcgc tcaacgggac cgatggagta tgaatgtaca gcaccaaatt tcgagctgtc
     7201 agtcaggact atcgcttcgc actctttttc cagcacggca ttgaccacat cggtacgcat
     7261 catatcgcga ccggtgaatc cagtttcagg ttgccagcca tcaataccaa taaatgcttt
     7321 gctgaaatgt acctgctgga tgcactggcg tgtcaaaggg ccaaccatac tttcgctttt
     7381 tttctggtac acgccaccga gcaaaatgac ttcacaaggc gcgtctttca gcaaatgcgc
     7441 aatgtagctg ctgactgtga tgatagtgac atttttcttc tgttcgccca gagtccgggc
     7501 gagcagggca ttgctgctac cattttcgat aaagatggtt tcgcccggtt gaaccagaga
     7561 cgcggcaaac tcggcgagtt cacgcttcag cgtatagttg ctcatcatac gggtttcgac
     7621 gtcatcacta tcaagcgaaa ctgcaaagcc atgtgcacgg cggaggtaac tcagtttttc
     7681 gagggtgttg agatcctggc gaatggtaac ttcagaaact ccagtggctt ttgccagatc
     7741 ggttacgcta acctgaccct ggtcaatgac catctgtaga atagtttgtt gtcgggaatt
     7801 catcgctgta attcttataa cgttataata ctaaataaaa aaatactgac gcgccgggca
     7861 tcctcaaata ggaaaacctg aaggcacgat tgttaaatct cccacggtgc cttcgggatg
     7921 tctggcgtag attcagcaac ctggcgcaag agttctgctt taagtatctc gagattatgg
     7981 atcgcagagt ggtaatcgcc tgccacaagg atatctaaca caatatcaat acgttcggca
     8041 acgcgctggg catcaaattc agacatcaag gacatcctta tagcaaaagt ggaaaatgaa
     8101 taatgcaaaa attgccgcta aaagagaagt gtttaacagc aacggctaat tatcatccag
     8161 gaatacgata tataatgacg ggatatagcg ctaagtatat atattcatct acttatgcgc
     8221 gcttcagata gcgtttatac cagcgttcga aggcgacggc gggcatcggt ttggcaaaca
     8281 aaaatccttg ccgctcattg atcccgttct tggttaaaaa agcatcttcc tttgcactct
     8341 ctacaccttc ggcgatcacc tgaagattca atgcctgggc cacagcgacg atcgcccgga
     8401 ccagtgactg cgagacaggt tgtttgtgaa tatctcgaac aaaaacctgg tcaagtttga
     8461 tggcatcgat cggaaagcgc gccagttgcg aaagtgaaga gtagccggta ccaaaatcat
     8521 ccagatgcac ttgcgcacct agttggctaa attgttgaat aacagacagt gccagttcat
     8581 cattctcaat cagacaactc tctgtcagtt caacatctat agggcagtat tcaaaattga
     8641 gttcctggag aacctgtttc agggcggtga aaatggtttg atcggcgagc tgacgtgcag
     8701 aaatatttac cgccactcgc aggtttatgc ctttatcccg ccactttgcc acctggcgta
     8761 cgacatcgag aatcacccaa cggcctaaag gcacaattag ccctgactct tcggcgtagg
     8821 aaatgaagtc caacggtgga atcaacccac gttcaggtga ctgccaacgt actagtgctt
     8881 ccagactgcg cacttcgcca cgccaggtga ttttcggttg atagtgaata accaactgat
     8941 cgttttccag tgctttacgc aagttggtat ccagccagag atattcaaat acccgttgat
     9001 tcatttctgg ggtaaaaacg caaaattgtc ctcgtccgcc ttccttcgct gtgtacattg
     9061 ctgtgtcggc gtgacgaata atagccgtgc tgtctgaacc atgttcggga gagagtgcaa
     9121 tacctactga acagctggta taaacttcaa ttaaaccaat gcgaaagggg agccgtaagc
     9181 gggtcaaaat tcgtgatgcc attgcttcca gcgcgctttg tgaggtgttg gatgccagta
     9241 ccagaaactc atccccacct ggacgcgcca acacctggtc atgttcgaga cagcttaaaa
     9301 tagccaatga cacgtcgcgt aataactggt caccaaacaa atgcccatag gcgtcgttga
     9361 cctttttgaa attatccaaa tcaagataca caaccccaac tttattgtta tctgcatgat
     9421 taatagcgtg atcgattaaa tcctgcattg cgttacgatt cggcagtccg gtgaaactgt
     9481 cggtatttgc cagaatacgc agtcgctcct gagcgcggcg ctcttcggta atgtcggtgc
     9541 cggaacagat taaaaaaatc tcgtttttgc cactgccgct gtggacaaat ttattgcgaa
     9601 acagaaacag ccgctggcct ttacatgttg gtatccacag ttcgacttca tatgcattgc
     9661 cgcttcgaaa aaatacacgg ttattgcgcc tggatgccgc agcttcacga cggctcataa
     9721 acagtttaaa cacgctttgc ccaatgacgt cgtgttcttt caaccctgtg taatcttcac
     9781 ataaccgatt gaagcgttgg atattccccc ggctgtcgag aatcactatt gcggaatgtg
     9841 cttcagaaac tacctgctca gcaaaggaaa gcccgtgtga taaatcacga gcaaccgccg
     9901 gtgtgtcatg ccatgcagac gcgttgccag cccattcccg tttattgatt tttcgtccta
     9961 ccagatgcac agaaagatcg ctttcatcga cggtcagact catcatcagg ctggaggtga
    10021 tgaccgtcat ttccctgatg cgagcagcct gttcggcaga taactgcaat gtacgatcag
    10081 gttctgtggt ttcggtggta gaaaagcgca aaacatcgct gctttccgtc aaccgccagt
    10141 atggattgtg cgatccgaga aagttgtaca acgttgtgga ctccctaacg gttttcatga
    10201 tgcatatcct ccggttaaca gcggagtccg cggttaaata aa
//
LOCUS       DAZGVQ010000174        10235 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000174 DAZGVQ010000000
VERSION     DAZGVQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.175"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..321
                     /locus_tag="ACHLAG_004362"
     CDS             <1..321
                     /locus_tag="ACHLAG_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HFP0657483.1"
                     /translation="GIFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVEGTWPNK
                     LQPGIYKNMGSNSVNIIINNTQKIIPPSKVFTLRGGSLNINIPGRSALLLGKTGEPPN
                     YLYL"
     gene            657..1634
                     /gene="glaH"
                     /locus_tag="ACHLAG_004363"
     CDS             657..1634
                     /gene="glaH"
                     /locus_tag="ACHLAG_004363"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFP0657484.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1653..2921
                     /gene="lhgO"
                     /locus_tag="ACHLAG_004364"
     CDS             1653..2921
                     /gene="lhgO"
                     /locus_tag="ACHLAG_004364"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFP0657485.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSELEMERM
                     RALWERTAANGIEREWLNAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2944..4392
                     /gene="gabD"
                     /locus_tag="ACHLAG_004365"
     CDS             2944..4392
                     /gene="gabD"
                     /locus_tag="ACHLAG_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP0657486.1"
                     /translation="MKLNDSKLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLLMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALSSKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4406..5686
                     /gene="gabT"
                     /locus_tag="ACHLAG_004366"
     CDS             4406..5686
                     /gene="gabT"
                     /locus_tag="ACHLAG_004366"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFP0657487.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGNFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYASS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            5924..7324
                     /gene="gabP"
                     /locus_tag="ACHLAG_004367"
     CDS             5924..7324
                     /gene="gabP"
                     /locus_tag="ACHLAG_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFP0657488.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7345..8007
                     /gene="csiR"
                     /locus_tag="ACHLAG_004368"
     CDS             7345..8007
                     /gene="csiR"
                     /locus_tag="ACHLAG_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFP0657489.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(8008..8457)
                     /gene="kbp"
                     /locus_tag="ACHLAG_004369"
     CDS             complement(8008..8457)
                     /gene="kbp"
                     /locus_tag="ACHLAG_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFP0657490.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8541..8699)
                     /locus_tag="ACHLAG_004370"
     CDS             complement(8541..8699)
                     /locus_tag="ACHLAG_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFP0657491.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8882..9181
                     /locus_tag="ACHLAG_004371"
     CDS             8882..9181
                     /locus_tag="ACHLAG_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP0657492.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            9191..9715
                     /gene="ygaP"
                     /locus_tag="ACHLAG_004372"
     CDS             9191..9715
                     /gene="ygaP"
                     /locus_tag="ACHLAG_004372"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFP0657493.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLSPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRT"
     gene            complement(9762..10166)
                     /gene="stpA"
                     /locus_tag="ACHLAG_004373"
     CDS             complement(9762..10166)
                     /gene="stpA"
                     /locus_tag="ACHLAG_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFP0657494.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2378 a         2611 c         2768 g         2478 t
ORIGIN      
        1 ggtatatttg caactaaaaa cgttctggcc tttagtcgtg gggattctgt gaacattaat
       61 cactctcctc atgatggact agttataata aataaaggaa acgaagaagt tgaaggtacc
      121 tggcctaaca aattgcaacc tggaatatat aaaaacatgg ggagtaatag cgttaacatt
      181 attattaata atacccaaaa aattatcccc cctagtaaag tatttacgct tagaggcgga
      241 tctctaaata tcaatattcc tgggcgtagc gctcttcttt tagggaaaac aggagaaccg
      301 ccgaactatc tctatttata atttaatgtt atatctgccc cgataaaacg gggcagataa
      361 tatgtttagt ttactaacgg tcattttgca gtgaagccat ttactgtttt ttatcgacca
      421 gataatctgt tctctaatgt taactccccc taacctgttg ctttagttat tcatttcctg
      481 tctcactttg ccttaatacc ctacgttaaa tgttactaat ttgttgcttt tgatcacaat
      541 aagaaaacaa tatgtcgctt ttgtgcgcat ttttcagaaa tgtagatatt tttagattat
      601 ggctacgaaa tgagcatcgc catgtcaccc tacatctcat aagaggatcg cttctgatga
      661 atgcactgac cgccgtacac aataacgctg tcgattcagg ccaggactat agcggattca
      721 ctctcatccc gtcggcgcaa tccccgcgtc tgctggaact caccttcacc gaacagacga
      781 ccaaacagtt tctcgaacag gttgccgaat ggcccgtgca ggcgctggag tacaaatcgt
      841 ttctgcgttt tcgggtaggc aaaattcttg acgatctgtg tgcgaatcag ctgcaaccat
      901 tgctgttgaa gaccctgcta aaccgtgctg aaggtgcgct gttgatcaat gcggtgggta
      961 tcgatgatgt ggcgcaggca gatgagatgg tgaagctggc aacggcggta gcgcatctga
     1021 ttggtcgctc aaatttcgat gcgatgagcg gtcagtatta cgcgcgtttc gtggtgaaaa
     1081 atgtcgataa ctcagacagc tatctgcgtc agccgcaccg cgtgatggaa ctgcacaacg
     1141 acggcacgta tgtcgaagag atcaccgatt acgtgctgat gatgaaaatc gatgagcaaa
     1201 acatgcaggg tggaaattcg ttgctgctgc atctcgatga ctgggaacat ctggaccact
     1261 atttccgcca tccactggcg cgtcgcccaa tgcgctttgc cgcgccgccg agcaaaaacg
     1321 tcagcaaaga tgttttccat ccagtgttcg acgttgatca acagggtcgc ccggtgatgc
     1381 gctatatcga ccagttcgtc cagccgaaag acttcgaaga aggcgtgtgg ttgagcgagc
     1441 tttcggacgc cattgaaacc agcaaaggca ttctttctgt acccgttccc gttggcaaat
     1501 tcctgttgat aaacaacctg ttctggctgc acggtcgcga ccgctttact ccgcacccgg
     1561 atctgcgccg tgaactgatg cgtcagcgcg gctacttcgc ttacgccact caccactacc
     1621 agacgcatca gtagcgcaaa ggaattgagc ggatgtatga ttttgtgatt attggcggtg
     1681 gcatcatcgg catgtcgacc gccatgcaac tgattgatgt ctacccggac gcccgcattg
     1741 cgttgctgga aaaagagtcc ggcccggcct gtcaccagac gggccacaac agcggcgtga
     1801 tccatgccgg ggtttattac acgcccggta gcctgaaggc acagttttgc ctggcgggaa
     1861 accgcgccac taaagccttt tgcgatcaaa acggcattcg ctatgacaac tgcggcaaga
     1921 tgctggtcgc cacctctgaa ctcgaaatgg aacggatgcg cgcgttgtgg gaacgcacgg
     1981 cggcaaacgg tatcgagcgc gagtggttaa acgccgtgga actgcgcgag cgcgaaccga
     2041 atatcaccgg gcttggcggc atttttgtgc cgtccagcgg cattgtcagc taccgcgaag
     2101 tcacagcggc gatggcaaaa attttccagg ccagaggcgg tgagattatt tataacgccg
     2161 aagtcagcgc cctcagtgag cataaaaacg gcgtggtcgt ccgaacccgt cagggcggcg
     2221 aatatgaagc atcgacgctg attagctgtt ccgggctgat ggctgaccgg ctggtgaaaa
     2281 tgctcggcct cgaaccgggt tttattatct gcccgttccg tggcgagtat ttccgccttg
     2341 cgccggagca taaccagatt gttaaccatc tgatttaccc cattcccgac ccggcaatgc
     2401 cgtttttggg cgttcatctc acccgcatga tcgacggcag cgtgaccgtc gggccaaacg
     2461 cggtgctggc tttcaaacgc gaaggctatc gcaagcgcga cttctcattt agcgacacgc
     2521 tggagatttt gggctcgtcg gggattcgcc gggtgctgca aaaccatcta cgctcaggac
     2581 tgggcgagat gaaaaactcg ctgtgcaaaa gcggctatct gcggctggtg caaaagtatt
     2641 gtccccggct ttcgttaagc gatctccagc cctggcccgc cggtgtgcgg gcgcaggcgg
     2701 tatcgccgga cggcaagctg attgacgatt ttctgtttgt caccaccccg cgcacgatcc
     2761 acacctgcaa tgcgccctcc ccggcagcga catcagcaat tcctattggt gcgcatattg
     2821 tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc cggacgcacg ctgcgagcgg
     2881 cacgtagtgt ggatgcctta cacgccgcat ttaatcaata acctttgaaa acaggacgta
     2941 acaatgaaac ttaacgacag taaattattc cgccagcagg cgttgattaa cggggaatgg
     3001 ctggacgcca acaatggcga ggtcatcgat gtcaccaatc cggcgaacgg cgacaagctg
     3061 ggtagcgtac ccaaaatggg cgctgatgaa acccgcgccg ctatcgacgc cgccaaccgc
     3121 gctctgcccg cctggcgtgc gctcaccgcc aaagaacgcg ccaacattct gcgcaactgg
     3181 ttcaatttgt tgatggagca tcaggacgat ttagcgcgcc tgatgaccct agaacagggc
     3241 aaaccgctgg ctgaagcgaa aggcgaaatt agctacgccg cctcctttat tgagtggttt
     3301 gctgaagaag gcaaacgcat ttatggcgac accattcctg gtcatcaggc cgataagcgc
     3361 ctgattgtta tcaagcagcc gattggcgtc accgcagcta tcacgccgtg gaacttcccg
     3421 gcggcgatga ttacccgcaa agccggtccg gcgctggcgg caggctgcac catggtgctg
     3481 aagcctgcca gtcagacgcc gttctctgcg ctggcgctgg cggagctggc gattcgcgcg
     3541 ggcattccgg ctggggtatt taacgtggtc accggttcgg cgggcgcggt gggtaacgaa
     3601 ctgaccagca acccgctggt gcgcaagctg tcgtttaccg gttcgaccga aattggccgc
     3661 cagttaatgg aacagtgcgc gaaagacatc aaaaaagtgt cgctggagct gggcggcaac
     3721 gcgccgttta tcgtctttga cgatgccgac ctcgacaaag ccgtggaagg tgcgctgtcc
     3781 tcgaaattcc gcaacgccgg gcaaacctgc gtctgcgcca accgtttata cgtgcaggac
     3841 ggcgtgtatg accgctttgc cgaaaaattg cagcaggcgg tgagcaaatt gcacatcggc
     3901 gacgggctgg agaaaggcgt caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa
     3961 gtggaagagc atattgccga tgcgctggag aaaggtgcgc gtgtggtttg cggcggtaaa
     4021 gcggatgaac gcggcggcaa cttcttccag ccgaccattc tggtggacgt tccagccaac
     4081 gccaaagtgt cgaaagaaga gacgttcggc cccctcgccc cactgttccg ttttaaagat
     4141 gaagccgatg tgattgcgca agccaatgac accgagtttg gccttgccgc ctatttctac
     4201 gcccgtgatt taagccgcgt cttccgcgtg ggcgaagcgc tggagtacgg catcgtcggc
     4261 atcaataccg gcatcatttc caatgaagtg gccccgttcg gcggcattaa agcctcgggt
     4321 ctgggtcgtg aaggttcgaa gtatggcatc gaagattact tagaaatcaa atatatgtgc
     4381 atcggtcttt aactggagaa cgcgaatgag cagcaataaa gagttaatgc agcgccgcag
     4441 tcaggcgatt ccccgtggtg ttgggcaaat tcacccgatt ttcgctgacc gcgcggaaaa
     4501 ctgccgggtg tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg ggattgcggt
     4561 gctcaatacc gggcacttgc atccgaaagt ggtggctgcg gtggaagcgc agttgaaaaa
     4621 actgtcgcac acctgcttcc aggtgctggc ctacgagccg tatctggagc tgtgcgagat
     4681 tatgaatcag aaggtgccag gcaattttgc caagaaaacg ctgctggtca ccacaggttc
     4741 tgaagcggtg gaaaacgcgg tgaaaatcgc ccgcgccgcc accaaacgta gcggcaccat
     4801 cgcttttagc ggcgcgtatc acgggcgcac gcattacacg ctggcgctga ccggcaaggt
     4861 gaatccgtac tctgcgggca tggggctgat gccgggtcat gtttatcgcg cgctttatcc
     4921 ttgcccgctg cacggcataa gcgaggatga cgctatcgcc agcatccacc ggatcttcaa
     4981 aaatgatgcc gcgccggaag atatcgccgc catcgtgatt gagccggttc agggcgaagg
     5041 cggtttctac gcctcgtcgc cagcctttat gcagcgttta cgcgctctgt gtgacgagca
     5101 cgggatcatg ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt
     5161 tgcgatggag cagatgggcg ttgcgccgga tcttaccacc tttgcgaaat cgatcgcggg
     5221 cggcttcccg ctggcgggcg tcaccgggcg cgcggaagta atggatgccg tcgctccagg
     5281 cggtctgggc ggcacctatg cgggtaaccc gattgcctgc gtggctgcgc tggaagtgtt
     5341 gaaggtgttt gagcaggaaa atctgctgca aaaagccaac gatctggggc agaagttgaa
     5401 agacggattg ctggcaatag ccgaaaaaca cccggagatc ggcgacgtac gcgggctggg
     5461 ggcgatgatc gccattgagc tgtttgaaga cggcgatcac aacaagccgg acgccaaact
     5521 caccgccgag atcgtggctc gcgcccgcga taaaggcctg attcttctct cctgcggccc
     5581 gtattacaac gtgctgcgca tccttgtacc gctcaccatt gaagacgctc agatccgtca
     5641 gggtctggag atcatcagcc agtgttttga tgaggcgaag cagtagcgcc gctcctatgc
     5701 cggaggcgac gctgcgcgtc ttgtccggcc tacggggatc caggtcggat aaggcgttta
     5761 cgccgcatcc gacaatctgt acgtgaacag gaagaaatct atgttggccg ggtaaggcgg
     5821 agccgctctc cggcaaaaag aatcaataac aattatacgc gtgacccggc gcgggaaatg
     5881 tcggggcgct ctcccaagtg acacactttc gagaggattc aggatggggc aatcatcgca
     5941 accacatgag ttaggcggcg ggctgaagtc acgccacgtc accatgttgt ctattgccgg
     6001 tgttatcggc gcaagtctgt ttgtcggttc cagcgtcgcc atcgccgaag cgggcccggc
     6061 ggtattactg gcctatctgt tcgccgggct actggtggtt atgattatgc ggatgttggc
     6121 ggaaatggcg gttgccacgc ccgataccgg ttcgttttcc acctatgccg ataaagccat
     6181 tggtcgctgg gcgggctata ccatcggctg gttgtactgg tggttttggg tactggttat
     6241 cccgctggaa gccaacatcg ccgccatgat cctgcactca tgggttccag gcattcccat
     6301 ctggttattt tccctcgtca ttaccctcgc cttaactggc agtaacttat taagcgttaa
     6361 aaactacggc gaatttgagt tctggctggc gctgtgcaaa gtcatcgcta tcctggcctt
     6421 tattttcctt ggtgcagtcg caattagcgg tttttacccg tatgccgaag tgagcgggat
     6481 ctcaagattg tgggatagcg gcggctttat gcccaacggt ttcggtgcgg tattaagcgc
     6541 gatgttgatc accatgttct cgtttatggg cgcagaaatt gtcaccattg ccgccgcgga
     6601 atccgacacg ccggaaaaac atattgtccg cgccaccaac tcggttatct ggcgtatttc
     6661 tatcttctat ttatgttcta tttttgtcgt cgtggcatta attccgtgga atatgcccgg
     6721 actaaaagcc gtcggttctt atcgctcggt actggaattg ctcaatattc cccatgcgaa
     6781 attaatcatg gactgcgtga tattactttc cgtaaccagt tgcctgaact cggcgctgta
     6841 taccgcgtca aggatgctct actccttaag ccgtcgcggt gatgctcccg cggtaatggg
     6901 taaaatcaac cgcagtaaaa ccccgtacgt ggcggtgtta ctctccaccg gcgcggcatt
     6961 cttaacggtg gtggtgaact attacgcgcc tgcgaaggta tttaaatttc tgatcgacag
     7021 ctccggcgct atcgccctgc tggtttattt agtcatcgcc gtttcacagt tgcggatgcg
     7081 caaaattctg cgagcagaag gaagcgaaat tcgcttgcga atgtggcttt atccgtggct
     7141 cacctggcta gtcatcggct ttattacctt tgtgttggta gtgatgctat tccgtccggc
     7201 gcaacagtta gaagtgatct ccaccggctt attagcgata gggattatct gtaccgtgcc
     7261 aattatggct cgctggaaaa agctggtatt gtggcaaaaa acacccgttc ataatacgcg
     7321 ctgatcatga tcaggagtca caccatgacc attacgtctc tggatggcta tcgctggctg
     7381 aagaacgata ttattcgcgg taattttcaa ccggatgaaa aattacgaat gagtttgctg
     7441 acatcgcgtt atgcacttgg cgttgggccg ttacgggaag ctctttcgca actggtggcg
     7501 gaacggctgg tcacggtggt gaatcaaaaa gggtatcggg tggcgtctat gtcagagcag
     7561 gagctgctcg atattttcga cgcccgcgcc aatatggaag cgatgttagt gagtctggcg
     7621 attgcccgcg gtggcgatga gtgggaggca gacgttctcg caaaagcgca tctgctgagt
     7681 aagcttgagg cctgtgacgc cagcgagaaa atgcttgatg agtgggatct gcgtcatcag
     7741 gcgtttcata cggcaattgt ggcgggctgt ggttctcact atttgctgca aatgcgtgaa
     7801 cggttgtttg atctggcggc gcgctatcga tttatctggc tgcggcgaac ggtgctttcg
     7861 gtagaaatgc tggaggataa acacgatcag caccagaccc tgactgcggc ggtactggcg
     7921 cgagataccg cgcgcgccag tgagttaatg cgccagcatt tactgacgcc aattcccatt
     7981 atccagcagg cgatggctgg caattaacta ctcttccgga atacgcaaca cttgccccgg
     8041 ataaatttta tccgggcttt ttagcatcgg tttattcgct tcgaagattt tattgtacag
     8101 attagcgtta ccgtagacct gtttggaaat ggcactcaga gtgtcgccag acttaacggt
     8161 ataaaactgg ctggcagtgg ctggtgtcgc cgttttcacc tgatcatcaa cactggcaat
     8221 accggaaata ttcccaaccg caacaaggat tttctccttc gcctcctgac tcaggccgtc
     8281 accagtgacc gtcgctttgc cgtcggcaat ttgaatgttc actttatcgg catcaggtat
     8341 accggttttg ctcagatgct cctgcacctt cttcgcctga tcgtctttat cgtgctgacc
     8401 tgtaaccgcg tcccagagtt tttctccggc atctttcaca aaattgaaca gacccatagc
     8461 tacctcattc gttaacggaa acactcagaa agtgtagcag aggaacgcgg cgcgttaacg
     8521 ccgcgggggt gagcacaggt ttaatcgcgg gtttgcaccc agaacgcgtg aatcaaaccg
     8581 gggatatacc caagcagtgt cagcagaata ttaataatga acgcccaacc gaacccttta
     8641 ccgagcagca cgccgagcgg cggcagaatg atggtgatga cgattctcca gaaacccata
     8701 tgtactccct ataagaaaat tactcattgt ttaaaaagag attttatctc ttaagcgtag
     8761 taattttagc ggagcctgcc agtttcagct tacgctttta ctctcattta cgctgctgag
     8821 gctggtatat gttgcatttt tagtttagag tttacttaat ttagaaaata cttaaataaa
     8881 tatgactgaa ctcgcgcaat tacaggccag tgccgaacag gcagcggcct tattgaaagc
     8941 aatgagccat cctaaacgat tgctgattct gtgcatgctt agcggttccc ccggcaccag
     9001 cgcgggagag ctgacgcgca ttaccggact gagtgcctct gcgacatcac agcatctcgc
     9061 tcgtatgcgg gacgaagggc ttatcgacag ccaacgggat gcccaacgca ttctatattc
     9121 cattaaaaat gaggcggtaa atgccattat cgccaccctg aaaaatgtct attgtccgta
     9181 aggagtcact atggctttga caaccatttc gccgcatgat gcacaagaat taatcgcacg
     9241 cggcgcaaag ttaatcgata ttcgtgacgc tgatgaatat cttcgtgaac atattcctga
     9301 agcagatctg tctccattat ccgtgctgga acagtcaggt cttccaccta aattacgtcg
     9361 cgagcaaatt atattccact gccaggcagg taaacgcacc agtaataacg ccgataaatt
     9421 agcagcgatt gccgcccccg cagaaatatt tttactcgaa gatgggattg atggctggaa
     9481 aagagctgga ttgccagtag cggtaaataa atctcaaccc ttgccgttaa tgcgccaggt
     9541 gcagatcgct gcgggtggtt taatattaat cggcgttgta ctgggttata ccgtaaatag
     9601 cggtttcttc ttattaagtg gctttgttgg tgccgggtta ctgtttgcag gaatcagcgg
     9661 tttttgtgga atggcaaggt tgttagataa gatgccgtgg aaccaacgaa cttgagaagc
     9721 gacgccggac gcgccctagc agcgacatcc ggcctcagta attagatcag gaaatcgtcg
     9781 agagatttac cttctgccag cgcctgagca attggcttcg gtgtacggcc ctgaccggtc
     9841 caggttttag tttcaccgtt aacatcggtg aatttatatt tcgccggacg cggctggcgt
     9901 tttttaccag cgcgtggtgc agcagcagag ctattaccta ataactcttc cgggttaatt
     9961 ccgtcagcct tcatcagctc cagccaggtg ctaatttttt cctgacgctc agccagttca
    10021 cgctgctgct gttcttcttc ttcacgtctt tctttagtga caaccctgaa tttttcgagc
    10081 atttcttcaa gaacgtcaat ggagaattcg cgagccatcg cacggagagt gcgaatgtta
    10141 tttaaacttt gtaacattac ggacataata aagaaaacct tttaacgcca aaacaaaaaa
    10201 gtatttcgtc gcgtagtgta agcgatcact tatta
//
LOCUS       DAZGVQ010000175        10223 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000175 DAZGVQ010000000
VERSION     DAZGVQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.176"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..513)
                     /locus_tag="ACHLAG_004374"
     CDS             complement(<1..513)
                     /locus_tag="ACHLAG_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0657495.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TLLLEAWKKYR"
     gene            complement(513..3926)
                     /locus_tag="ACHLAG_004375"
     CDS             complement(513..3926)
                     /locus_tag="ACHLAG_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP0657496.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSQPGTLNDFLGAMTEDDARPEA
                     LRRFEVMVEEVARNASAVAQNTAAAKKSAGDASTSAREAATHATDAADSARAASTSAG
                     QAVSSAQSASSSAGTASAKATEASKSAAAAESSKSAAATSAGAAKTSETNASASQQSA
                     ATSASTATTKASEAATSAREAAASKEAAKSSETNASSSASSSASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASASATAAGKSAESAALSA
                     STATTKAGEAAEQASAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKNTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSSSETLAATPKAVKSAYDNAEKRLQKDQNGAD
                     IPDKGRFLNNINAVSKTDFADKRGMRYVRVNAPAGAISGKYYPVVVMRSAGSVSELAS
                     RVIITTATRTAGDPMNNCEFNGFVMPGGWTDRGRYAYGMFWQYQNNERAIHSIMMSNK
                     GDDLRSVFYVDGAAFPVFAFIEDGLSISAPSADLVVNDTTYKFGATNPATECIAADVI
                     LDFKSGRGFYESHSLIVNDNLSCKKLFATDEIVARGGNQIRMIGGEYGALWRNDGAKT
                     YLLLTNRGDVYGGWNTLRPFAIDNATGELVIGTKLSASLNGNALTATKLQTPRLVSGV
                     EFDGSKDITLTAAHVAAFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVNFYT
                     GVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPS
                     DTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSLSGSTGSAGAHTHGNGIRWP
                     GGGGSALAFYDGGGFTYVQDSQYQVSPGTSSSRSYYQRIQTQSAGAHTHSLSGTAANA
                     GAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            complement(3991..4590)
                     /locus_tag="ACHLAG_004376"
     CDS             complement(3991..4590)
                     /locus_tag="ACHLAG_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP0657497.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHVSTNVPG
                     SDALNGINVKYRYEFTDTLGMVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            complement(4657..8052)
                     /locus_tag="ACHLAG_004377"
     CDS             complement(4657..8052)
                     /locus_tag="ACHLAG_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0657498.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDTEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQNCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFRQ
                     LALGRYTLTVRAVNAWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNIVGKS
                     AFVESVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWAVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGRLTAKNADISGNVNANSGT
                     LNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPF
                     DRQIVIPAVAFSGAKHEREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPA
                     GHGHMTLEFSVSAWLVNDWYPTASISDLLVVVMKKATAGISIS"
     gene            complement(8113..8760)
                     /locus_tag="ACHLAG_004378"
     CDS             complement(8113..8760)
                     /locus_tag="ACHLAG_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0657499.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGSEAIRAL
                     AMQIPAFRQKLSDGWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(8658..9401)
                     /locus_tag="ACHLAG_004379"
     CDS             complement(8658..9401)
                     /locus_tag="ACHLAG_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0657500.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGEKYFPCVNISGEPE
                     EYFRMSPEDWLRAEMQGEIVALVHSPPGGLPWLSETDRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLSGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDSELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFE"
     gene            complement(9407..10105)
                     /locus_tag="ACHLAG_004380"
     CDS             complement(9407..10105)
                     /locus_tag="ACHLAG_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0657501.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLAEVGGERYFSCNEQNE
                     KGEPVIWQGRQYQVYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            complement(10115..>10223)
                     /locus_tag="ACHLAG_004381"
     CDS             complement(10115..>10223)
                     /locus_tag="ACHLAG_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0657502.1"
                     /translation="PPYDWKPIRVVCRKWSASVGALWVTITADFEQVVA"
BASE COUNT         2142 a         3113 c         2552 g         2416 t
ORIGIN      
        1 ccgatacttt ttccaggctt ccagcaacaa ggtttcttcc tccgttgcga tctccagatc
       61 cacagcatcc tgaagcggcg ctatatgctc actggctacc tgcatcaggc tgttttttgt
      121 ttcttccgcc tcccggatcc ggaacagttt ttctgcttcc gtatccttca cccaggctgt
      181 accgttccac ttctgaaact ccccttccgg ggataaccag gtgacatttt ccggtaatga
      241 gccgagttca gaaataaata acgcgtcgcc ggaagccacg tcatagaccg tttttccccg
      301 atggtcttca acgagatgcc acgatgcctc atcactgttg aaaacagcca caaagccagc
      361 cggaatatct ggcggtgcaa tatcggtact gtttgcaggc agacctgtat gaggaggaat
      421 atatgcgtca ccttcaccaa taaattcatt agttccggcc agcaggttat aaatttttat
      481 ggtccgtgct tgttcactca ttctgaatgc cattatgcga gcctcacaat atagttaaat
      541 gcgatgtttt tgacggtgtt ttccgcgtta ccagcagcgt taacggtgat ggtgtgtcca
      601 tgtgacccaa tcgcaaccga gtgcgtatgc gcaccaatac ctacagtatg tgcatgtgcg
      661 ccagcatttg ctgcagtacc agacagcgag tgggtatgag cacctgctga ctgtgtctga
      721 atacgttgat aatacgatct actggaagaa gtccccgggc ttacttgata ctgtgaatcc
      781 tggacataag tgaacccacc gccatcataa aatgctaacg cagaaccgcc gcctcctggc
      841 caacgaatac cattaccatg agtatgagca ccggcagacc ccgtagagcc actcagactg
      901 tgcgtatgcg ccccggtgtt attcgtggat ttagtgccgt aatcaaacga cgatgtggtt
      961 ttcgtcccca aatccgtact ggatgcgctg gcgctgtggg tatgcgattt aatgccatcc
     1021 tgttcctgag acaatacggc acgaccactg gcgggcttgc ccttaatcgt ccagccacgc
     1081 atatcaggga tcacgcctga cggataagcg gctgcaagtt tcgggtaggc agatttgtca
     1141 aaagtctgcc cctgcatcag ggcataacca gacggaacgg tatctgatgg ccacgggatt
     1201 ggtgcgccga ctgggtagct ttctggtgga agatttttcg aggtataaac ttctgcccag
     1261 tcttcctcaa aaccatagcc atctcttgaa gaacggtaga acagaccacc atttctgtaa
     1321 tgcgccttca tctgcagggt ccggcaactt ccgactccgg tatagaagtt aaccagaata
     1381 tagctgtcgc cagagcgggt gacattgtaa gcgcctgatt cggcattcca gggaacgcca
     1441 ccatccgcat cggcatatgt atccgttgcc cttctggcaa aagcagccac atgcgcggcg
     1501 gttaaagtaa tatctttgga accatcaaac tcaacaccag aaaccagtct tggcgtttgc
     1561 agctttgttg ctgttaatgc attaccgttc agacttgcgg acagtttggt tccaataacc
     1621 agttcgccgg ttgcgttatc aatagcaaac ggtcttaatg tattccagcc accataaaca
     1681 tcacctcgat tggtaagcag caggtaagtt ttagcgccat cattacgcca taatgcacca
     1741 tactccccac ctatcattcg aatctgatta ccaccacgcg ctacaatttc gtctgtggca
     1801 aaaagttttt tgcacgacaa gttatcgtta acaattaacg aatgagactc ataaaaacca
     1861 cgcccactct taaaatcaag gataacgtcc gccgcgatac attcagtcgc cggatttgtt
     1921 gccccaaact tataggtcgt atcattaaca acgagatcag cactaggtgc ggatattgac
     1981 aggccatctt cgataaacgc aaaaacaggg aaagcagcgc catcaacata gaacacagag
     2041 cgcaaatcat cgcccttatt actcatcatt attgagtgga tggctcgttc attgttttga
     2101 tattgccaga acattccata agcataacgc cccctgtcag tccagccacc aggcataaca
     2161 aatccgttaa actcgcagtt attcatcgga tcgcctgcgg ttcgcgttgc cgtggtgata
     2221 atgacccttg atgccagttc gcttactgag ccagcagaac gcataacaac aacagggtaa
     2281 tattttccag atattgcacc tgcaggagcg ttaacccgca cataacgcat accacgctta
     2341 tcagcaaagt ctgttttact gaccgcgtta atgttgttca ggaagcgtcc cttatcgggt
     2401 atatcagcgc cgttctggtc tttctgcaga cgtttctctg cattgtcata ggctgatttt
     2461 actgcctttg gcgttgccgc cagcgtttca gacgaactgt tggtcgcact gctgagctgt
     2521 actatcccct ttttcgtcgt acttgcatcc tcaagcgcca cggcggatgc aatatcctct
     2581 gcccgtttag ctgctgtctc ggcgcgcgtt gctgcggatt ctgccgtatt cttgctctgt
     2641 gctgccgccg tcgcactgcc agcagcctct gttgccttcg tggatgccgt cgtggcgctg
     2701 cctttcgctg ctgatgcctg tctggtcgcc tcatcttttg aagcagacgc cgatgaggcc
     2761 gatgacgccg ccgaactggc ggacgatgcg gcagccgttt ttgaggattc tgcgctggtt
     2821 tccgacgctt tcgcgttcgt ttcggatgtc ttcgctgcgg aagctgacct cgctgctgcg
     2881 ctggcctgtt cagcggcttc gccagccttc gttgtggctg ttgaagcgga caatgcagcg
     2941 ctttctgccg attttccggc ggcagtggca ctggctgagg cctgcccggc acttgttgac
     3001 gcggcactgg cagatgatgc agccgctgtt tttgagcctg ccgcagctga ggcgctctgt
     3061 cccgctgccg tctcagaaga cctggcgttc gtctcggacg tttttgccgc cttcgcggaa
     3121 tttcctgccg ccgttgccga ggaagcggaa ctactggcgc tcgaggatgc gttcgtttct
     3181 gatgatttcg ctgcctcttt tgaggccgcc gcctcccggg ctgaggtggc agcttctgac
     3241 gctttcgtgg tcgcggtgga tgcagaagtg gctgctgatt gttgtgacgc tgaagcattc
     3301 gtttctgacg tttttgccgc accggcactg gtggccgccg cgctttttga ggactctgca
     3361 gcggcagcac tttttgatgc ttcagtggcc tttgctgatg ccgttcctgc gctggaagac
     3421 gctgactgag ccgacgacac ggcctgtccg gctgacgtgc tggctgcgcg tgctgagtct
     3481 gcagcatcag tcgcatgggt tgccgcctca cgggctgatg tgctggcatc gccggctgac
     3541 ttcttcgcgg ctgccgtgtt ctgtgccact gcggacgcgt tacgcgccac ctcttccacc
     3601 atcacttcaa aacggcgcag tgcctccgga cgggcatcat cctccgtcat ggcaccgaga
     3661 aaatcattca gcgtaccggg ttgagaatct tcatacacgg tgatagtccc ggcatgtgac
     3721 ggcgggaatc cctccaccaa cagaataacg ctgtactgac catactcaac gtccatactg
     3781 taacgcccgg cttcatccgg attttctgag gccagcgtgt tcaccaccac cgtggtgctg
     3841 ttacgttttg ctttcagctg gattgtgcag ttctgtaccg gttttcctgt gccgtctttc
     3901 agtacacctg aaatctttac tgccatattc accccacaaa aaagcccgcc tgaaccggcg
     3961 ggctgtcata acactgtgtt acctggctaa tcagaattta taaccgacac ccacgatgaa
     4021 accgtcagtg cgccagtcgc cactgccgga gccttcataa gcaatatcaa tggccacgga
     4081 ttcggtcggg ttaaactgca cgccagctcc ccacgccaga gacgtgttgc tgtggcgacc
     4141 gtcatcactt ccggtcagca catcatgcgt tttccccttg ttgtcagtta cgcggagata
     4201 atccccggaa aaagtcgaca cacggctgta agccacgccc gccatcgcat acgcactgaa
     4261 ccattcattc acgcgcacag agggccccgc cattacgctg aaccagcggt tacgaacgga
     4321 atcttcatgc cagcgggtat cgctgtaacg ggtaagctgg cgattcttgt ctcctgcata
     4381 gctgaaagac gtcaccatcc ccagtgtgtc cgtaaactca taacggtatt tcacgttaat
     4441 cccgttcaga gcatcgctgc cgggaacgtt cgtcgagaca tgaagatacc ccgcgctcag
     4501 cgtggactga tgttcagatg cccatgcagg cgcaccggat acggccagac aaatggctgc
     4561 ggacaaaatg gcggcataaa gtttacgcat aattacctct cgcttttctg caaaaaaaag
     4621 gcaccatttc tggtgcccgt atatgggtta taaaattcag ctgatactga tgcctgcggt
     4681 ggctttcttc atcaccacaa ccagcaaatc gctgatactt gctgtgggat accagtcatt
     4741 taccagccat gctgacaccg aaaactccag cgtcatgtga ccgtgaccgg caggcatatc
     4801 aataacgcca ctgtaaatca gcgtattatc cagcgcggta cggttataaa tttcagcacc
     4861 gtttttccgc actatcagac ggcatgagga gtaaatatca gtatgctctc tctcatgttt
     4921 agcgccgctg aatgccaccg ccggaataac aatctgccgg tcaaacggct gatcgtcata
     4981 aaccctgacg gtaatggtcc ctgatggcca ccgttccggt gcacgggagt cccgggggaa
     5041 agctttgccc actgttttaa cgagatcgcc ttcaatctgg ttcgcggaca gttttcccag
     5101 aacccgacag ttctcgttaa tcgtgacgtt gttgagcgtc ccggagttcg cattcacgtt
     5161 accgctgata tcggcatttt tcgccgtcag ccgcccatcc ggtgtcaggg aaaatgccgg
     5221 tggatttcca ccactggtaa tggtgggggc cgtcaggcgt ttcaggaaca cgtcgttcat
     5281 gaatatctga ttgccctgcg ccacaaacat cggcgtttca ttcccgtttg ccgggtcaat
     5341 aaacgcgata cggttagcgg caaccagaaa ctggctcagt ttgccttcct ccgtgtcctc
     5401 catactgagg ccaatacccg cgacataatg tttgccgtct ttggtctgct caattttgac
     5461 agcccacatg gcattccatt tatcgttggc atccttccac tctttcgaaa actcatccag
     5521 tctgctggcg ttatcctccg tcagatcgac tttttccagc agctccttgc cgagatggga
     5581 ttcggttatc ttgcctttga aaaaatccag gtaaccttcc gcatcatcgc tcgcccgacc
     5641 gacggactcc acgaatgccg atttgccaac gatgttcaca ctgcggatat aaaagtaata
     5701 atcatggccc ggtttgatat tgatactggc ggctatccag tacagccccg tgccaaggta
     5761 gcgggctgtg gtttcaacct gcctgatatc ggtaatccgc gtttccgaga accagaactc
     5821 aaactgtacc gtcggatcat aaaccgcaag atgcggcgtg gcggtgatct gaaaatagcc
     5881 cggtgtcagc tcaatccgcg acggcgcagc cggtgcggca atccggaacg ataccgatgc
     5941 cggatcgccc tgctgtcccc acgcatttac cgcccggacc gtcagcgtgt aacgccccag
     6001 cgccagttgc ctgaagcggt atgtggtttc cgtcgtccgg gccgtgctga ccagccgctc
     6061 actgccgtca tccgctgtta cggtcagacg gagcaggaag ctcacgccct tcaccacctt
     6121 cggcgtgtcc cagcgcgcca gcacctgata ttccccgctg tctgcggtga cttcggcggt
     6181 caggtgctgc accgctggcg gcgtgacacc attcaccgtg ccgctctggt cgccgtcaaa
     6241 gtgcgccccg ttatccacga tggcctcttt ttccggtaca tgctgcacgg cagtgatggc
     6301 atacgtgccg tcatcgttct cacggatact cacacagcgg aacaggcgct ggcgcaacgt
     6361 cggcaacttc agcccccaca cgctgtattc tgcaacgccg tcaggaacac ggctcacttt
     6421 caccttcacg ccgtcggtga cggactggac ctccacgctg accggattgc cacttccgtc
     6481 aaccaggctt atcagcgtgg taccggagga tggcagcgtg atttcacggt cgagcgtcag
     6541 cgtccgggtc tggctgttca ccgccagcac gcgcccgccg atgctgatcc ccgcatagtc
     6601 atcatcgcag atttcaatga catcgcccgg tacatggcga agcccttctg cgcccacgct
     6661 gaagtccacg gtctgcgtct ccagcagctc cgttttaatc agccacagcc cggcgcggtg
     6721 tgcctgcccc cgactggtac aaccaaaggc atccatcttc gtgacattac gaccgtaacg
     6781 ggcaatggcc tgcgtatctt caacaagctc tgtcgccgtc tcccagccgt tgttcgggtc
     6841 aatccagttc acctcaacgg cattatggcg gtccttcagg gcgctgaagc tgtagcggaa
     6901 cggcgcgcca tcatccggca tcaccacatt actgcggtta taggtccaca ccttatccga
     6961 tggccggtcc tgcacgaacg tcagcgtctg cccgttccat accggcatac agcgcatcgc
     7021 cgagcagaaa tcactgagca catcccacgc cttgcgctgt gtggtcagcc aggcattaca
     7081 ggtgatgcgc ggctccgtgc cgccaaagcc gtccggcact gactggtcgc aattctggcc
     7141 gatgacatac agcgcccatt tatccacatc cgccgcacca agacgtttcc ccatgccgta
     7201 gcgcggatgg gtcagcatat cccacagaca ccaggccatg ttgttgctgt atgccggttt
     7261 taacgttccg tcccagatac cgctgtattg ccgcgtctgc gggttatagt tcgacggcac
     7321 ctgcagaatg cgcccgcgaa gatgataatt acggctcacc tgctggctgc cgaactgctc
     7381 cgagtccacc tgcacgccga ccagtgccgt gttcgggtag cactgtttca catcgatgat
     7441 ttcggtgtat gacgaccaga gcgttttgtt ctgcagctgg tctgtggtgc tgtccggcgt
     7501 catcctgcgc atccggatat taaacgggcg cggcggcagg ttatccacca ccaccgaggc
     7561 cagatactgc gaggtggttt tgcccttaat ggtgatgtct ttttccgtca cccagccacc
     7621 gttacgctgt atctgaacca gcaggcggac ttccgatgga ttcctgtccc cctttgaggt
     7681 ggtttccacc agtgcctgca caccgaaggt aaagcgcaga cggtcgatgt ttgccgacgt
     7741 gatggtgcgg gtgatcggcg tgtcatattt cacttccgta cccagcaccg tctcggaacc
     7801 ggaggattca aacccctccg gcggagtctg ctcctgctca ccggcccgga acaccaccgt
     7861 gacgccggag atgttggtat tcccctcagt gtccagcact ggcgtactgt tcagcagcac
     7921 gctttttaat ccatccaccg gaccttcaat cggcccttcg ctgatggcat cgatcacact
     7981 cagcaactgc gtggacttca ggttgtcctt cgcttcgcgc ggagtatgcc ccttactgct
     8041 gcctttaccc attcctcacg ctccataaac gacaaaaccg cccggaggcg gtttcacata
     8101 aaacgttttt catcagcgac caatcaccac aacctgacca ccatcccctt cgtctgccgt
     8161 gctgatctcc tgagagacca cccgcgaccc cacgcgcatt tcaccgtaca gaacgggcag
     8221 aacattgccc tgggcaacca tgttatccag tgaggagaaa taggtgttct gtttgccgtt
     8281 atctgtactt gctgccgtgg gcgtcctggc tttcggtgcc agcatctgcg ccactccgcc
     8341 caggatcata ctggcccctg ccgcatacat gcccgataca gccgcggcac ccagccagcc
     8401 cacagggttc caccatgcca ccgcaatcag cgccgccccc agcaccacct gaaacacacc
     8461 gccactttta gctcccgcca gacgcggcac gatgtggatc acggcaccat ttgccagcgg
     8521 ctcattaaga cgggcagaca attcggtttc acctgcatca cgcccggcaa tgcgtacctg
     8581 gtaccagccg tcgctcagtt tctgacgaaa cgccgggatc tgcatggcca gcgcccggat
     8641 ggcttcggac cccgttttca ctcgaaggtc gatgcggcgg ccaaatcgtt gtaaatcccc
     8701 gtaaaggcag atgcgtgcca tgcccggtga cgccagaggg agtgtgtgcg tcgctgccat
     8761 ttgtcggtgt acctctctcg tttgctcagt tgttcaggaa tatggtgcag cagctcgctg
     8821 tcaccacagt aaatggcggc atgattcggc accgatgaac caaaacagca cagcagcaca
     8881 tcgcccggct gcgccgctga caacggcacc tgatacagcc ctgtggcctc cagattatcc
     8941 agatagagat tctgaccgtt acgccaccag tcatcctcgc gatgaaaatc cggcatctca
     9001 atccccgcca gatggtaagc atcccggaac agcgtgtaac agtccgtcac cccgtgctca
     9061 aagcgccgcc cggaaagatg tggtacacag cggaacttgt gaatcgcccc ccggcaaacc
     9121 agccaccacg gcaaatcact ctgcacctgc agccgccggt cggtctcact cagccagggc
     9181 agaccaccgg gggggctgtg gaccagcgcc acaatctcac cctgcatctc tgcccgcagc
     9241 cagtcctccg gcgacatccg gaaatactcc tccggctcac cggagatatt cacgcaggga
     9301 aaatattttt ccccctccgg tgttctcacc acgaagccgc acgactccgc tggcgcacat
     9361 cgccgggcgt gcgccagaat cgctgattct gtctctgtca tgggattcac tgcgaaagtt
     9421 tgttaatgga aaggaagccg ccaaagttgc cgacgttatt gcgaaactta cagccgctca
     9481 ggcatttgct gcatttatcc ttcgtgatat cggacgtcgg ctgatcatat tcatccgcga
     9541 ccgccggacc gtgataaccg cactcatcac cgcgataggt ccaggtgcag gtgttggcca
     9601 gcatgatacg tcccggaaaa acggcaccgt ccgtttccgt cggcgtggac agtacaaaag
     9661 aggcactcac cgcgctcagt tcgctgcact gctcgatgcg ccagcggctg atcacctcct
     9721 gctccggatc ggcgtcactg tttccgttga cgaagttcac cgcatccaga aaacgggcgt
     9781 aaaccttacg ccggaccacc gttcctccga ccagactctg caggtcttcc gccatcccgg
     9841 tgaccatgcc gtgcaggtta gaaaccgtca gtgtcggacg ggcagcactg cccttgccgt
     9901 tcagttcaaa tcccgtcccc tgaatggggt atacctgata ctgccgcccc tgccagataa
     9961 ccggctcacc tttttcgttc tgctcattac aggaaaaata acgttcacca ccgacctctg
    10021 ccagatcgat ttcccatagc accacctggg ctgactgagt gaggcgtgtc gtctcatgat
    10081 gtgtttcctg tggaatatcc tgcatcagag cctcctatgc cacgacctgt tcaaaatctg
    10141 ccgttatggt tacccacagc gcccccacgc ttgccgacca tttacgacaa accaccctga
    10201 tcggtttcca gtcataaggt ggc
//
LOCUS       DAZGVQ010000176        10164 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000176 DAZGVQ010000000
VERSION     DAZGVQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.177"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            244..1617
                     /gene="aroP"
                     /locus_tag="ACHLAG_004382"
     CDS             244..1617
                     /gene="aroP"
                     /locus_tag="ACHLAG_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFP0657503.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTHFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1660..2514)
                     /gene="ampE"
                     /locus_tag="ACHLAG_004383"
     CDS             complement(1660..2514)
                     /gene="ampE"
                     /locus_tag="ACHLAG_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFP0657504.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPRAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2511..3062)
                     /gene="ampD"
                     /locus_tag="ACHLAG_004384"
     CDS             complement(2511..3062)
                     /gene="ampD"
                     /locus_tag="ACHLAG_004384"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFP0657505.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3150..4043
                     /gene="nadC"
                     /locus_tag="ACHLAG_004385"
     CDS             3150..4043
                     /gene="nadC"
                     /locus_tag="ACHLAG_004385"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFP0657506.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4246..4686
                     /gene="ppdD"
                     /locus_tag="ACHLAG_004386"
     CDS             4246..4686
                     /gene="ppdD"
                     /locus_tag="ACHLAG_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFP0657507.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            4696..6081
                     /gene="gspE"
                     /locus_tag="ACHLAG_004387"
     CDS             4696..6081
                     /gene="gspE"
                     /locus_tag="ACHLAG_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFP0657508.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6071..7273
                     /gene="hofC"
                     /locus_tag="ACHLAG_004388"
     CDS             6071..7273
                     /gene="hofC"
                     /locus_tag="ACHLAG_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFP0657509.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7308..8351)
                     /gene="guaC"
                     /locus_tag="ACHLAG_004389"
     CDS             complement(7308..8351)
                     /gene="guaC"
                     /locus_tag="ACHLAG_004389"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFP0657510.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8507..8551)
                     /locus_tag="ACHLAG_004390"
     CDS             complement(8507..8551)
                     /locus_tag="ACHLAG_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657511.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8576..9196
                     /gene="coaE"
                     /locus_tag="ACHLAG_004391"
     CDS             8576..9196
                     /gene="coaE"
                     /locus_tag="ACHLAG_004391"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFP0657512.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFLSQEKP"
     gene            9196..9939
                     /gene="zapD"
                     /locus_tag="ACHLAG_004392"
     CDS             9196..9939
                     /gene="zapD"
                     /locus_tag="ACHLAG_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFP0657513.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9949..10146
                     /gene="yacG"
                     /locus_tag="ACHLAG_004393"
     CDS             9949..10146
                     /gene="yacG"
                     /locus_tag="ACHLAG_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFP0657514.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
BASE COUNT         2496 a         2674 c         2602 g         2392 t
ORIGIN      
        1 ttctacatat tgagaggggt tgaggctgag ctttacaaac ggtttctttt taagcaactc
       61 atcttcaacc atgcataaag tgggtgcatt cgctgccgca taccattatt cttgatctga
      121 cggaagtctt tttgtaacaa ttcaaacttc tttgatgtaa acaaattaat acaacaaacg
      181 gaattgcaaa cttacacacg catcactgcg tagatcaaaa aaacaaccac cgcacgaggt
      241 ttcatgatgg aaggtcaaca gcacggcgag cagctaaagc gcggccttaa aaaccgccat
      301 attcagctta tcgcgctggg tggcgcgata gggaccgggt tattcctggg tagcgcctcc
      361 gtaatacagt ctgcagggcc agggattatc ctgggttacg ccattgctgg ttttatcgcc
      421 tttctgatca tgcgtcagct gggtgaaatg gtggtcgaag aacctgtcgc aggctccttt
      481 agccactttg cttataaata ctggggcagt tttgccggtt tcgcctctgg ctggaactac
      541 tgggtactgt acgttttagt tgccatggct gagctgactg ccgtgggtaa atacattcag
      601 ttctggtatc cggaaatccc cacctgggtt tctgccgccg tattctttgt ggtgattaac
      661 gccatcaacc tgaccaacgt taaagtgttt ggcgagatgg agttctggtt tgccattatc
      721 aaagttatcg cggtggtagc gatgatcatc ttcggcggct ggctgctatt cagtggcaac
      781 ggcggcccgc aggcgaccgt tagcaacctg tgggatcagg gtggtttcct gccgcacggc
      841 ttcaccgggc tggtgatgat gatggcgatt atcatgttct cgttcggtgg tctggaactg
      901 gtggggatca ccgcagcaga agctgataac ccggagcaaa gtataccgaa agcaactaac
      961 caggttatct accgcatcct gattttctat attggttcgt tagccgttct gctctcactg
     1021 atgccgtgga cccgcgttac cgccgatacc agtccgtttg tgctgatctt ccacgagtta
     1081 ggcgatacct ttgtggcgaa tgcgctgaac atcgtggtac tgactgcggc gctctccgtg
     1141 tacaacagct gcgtatattg caacagccgt atgctgtttg gtctggcaca acagggtaat
     1201 gcgccaaaag cgctggcgtc tgtcgataaa cgtggtgtac cagtaaatac cattctggtg
     1261 tctgcactgg taacggcgtt gtgcgtactg attaactacc ttgccccaga gtccgctttc
     1321 ggactgttaa tggcgctggt ggtatctgca ctggtaatca actgggcgat gattagcctg
     1381 gcgcatatga aattccgtcg cgccaagcag gaacaaggcg tggtaactca cttccctgct
     1441 ctgctttatc cgctgggtaa ctggatctgc ctgctgttta tggcggcggt actggtgatt
     1501 atgctgatga ccccaggaat ggcgatttcg gtatacctga tcccggtatg gctgatcgtg
     1561 ttaggtatcg gctatctgtt taaagagaaa accgccaaag ccgtaaaagc gcattaatct
     1621 ctctacgccc tcacccgtac agggtgaggg caataatctt tacaccaacg ccccgtaaat
     1681 cgtcagtagt gcaatcacca ccacgaccac gaacgaggtt ttcttcgcca ttgaaaccgc
     1741 tgccctcggc gtctccacct tatcgacatg cggttcacgc gccagagaga actgcgccag
     1801 acgcgttaac acctgatact gcgaagtatg gaaatcaccc agcgaagcaa accaggccgg
     1861 taacgctttc tcaccatgac cgatcaaggc atataccaca cccgcaagac gaaccggcac
     1921 ccaatccagt acatgaagca cggcatcaat gccggactgt aaacgatgat gcggcgtctg
     1981 atatcgcgcc agccagtatt gccatgcacg caaaaacgca taccccatca gcgtaacggg
     2041 tccccaggtt ccccccacaa tcagccagaa cagcggtgca agataaaaac gaaagttaat
     2101 ccacagcaat gcattttgca gctcacgcaa atactcacgt tcgtcgcagc ctgccgggac
     2161 gccgtgaatc atggtgagtt cgccagccat cgtggcacgg gcatggctat cattacgtga
     2221 agcagctgtc agataagcat gataatgaag acgaacttta cctgcgccaa tacacagcaa
     2281 accaatcagc agccacacca gtagcgtggg aacgttgaac aatactccct gcaatgcgcg
     2341 taacagtaaa aaagtcacgc ccatcgcaat aatggtcatg cctaacgtgc gcccgagaga
     2401 aaaatgtttc acccgccgaa agaacgcttc aagacgatga tcaagctgcc agtgctcgcc
     2461 caacttaaac aggcgctcga aaattaacac cagtaaggtt gtaaatagcg tcatgttgtc
     2521 tccttgctga ccagcacacg aaaccgtgcc caatcaaatg caggaccggg atcggttttc
     2581 cgatccggcg caatatcaca atggcccgtc atgtttttag cgatatccgg atagcaatca
     2641 atcagtgccc gcgtaaccgc cgcaagctgt tgatactgcg catcggtata cgccagcgta
     2701 tcggtgcctt caagctcaat cccaatagaa aaatcattgc agcgttcgcg cccctgatac
     2761 tgagagactc ccgcatgcca tgcacgttta tcgaaaggaa catactggac tatttcacca
     2821 tcacggcgaa tcaaacagtg agcggagacg cgcaaatggg cgatctcagc aaagaaagga
     2881 tgtgcctgcg gatcaatagt tccagtgaat aatgcgtcga tccacggacc gccaaactcg
     2941 cctggcggca ggctaatatt gtgcaccacc agcagggtgg gtgtttcgtc atccgggcgg
     3001 caatcgtaat gtggtgaggg aacgcggcgc gcgccaacca gccacccctg ttctaacaac
     3061 atgcaggatc tccttatatg tggtgctaat acccggttca gagtagcatg tttctacctt
     3121 atgattcgtt agctatctgg agttttaaca tgccgcctcg ccgctataac cctgacaccc
     3181 gacgtgacga gctgctggaa cgcattaatc tcgatatccc cggcgcggta gcccaggcgc
     3241 tgcgggaaga tttaggcgga acagtcgatg ccaacaatga tattacggca aaacttttac
     3301 cggaaaattc tcgctctcat gccacggtga tcacccgcga gaatggcgtc ttttgcggca
     3361 aacgctgggt tgaagaggtg tttattcaac tggcaggcga cgatgtcacc ataatctggc
     3421 atgtggatga cggcgatgtc atcaatgcca atcaatcctt gttcgaactt gaaggcccat
     3481 cccgcgtgct gttaacgggc gaacgcactg cgcttaattt tgtgcaaacc ctttcaggag
     3541 ttgccagtaa ggtacgccac tatgtcgaat tgctggaagg caccaacacg cagttgttgg
     3601 atacgcgcaa aaccttaccc ggcctgcgtt cagctctgaa atacgcggta ctttgcggcg
     3661 gcggagcgaa tcaccgtctg gggctttctg atgccttcct gatcaaagaa aaccatatta
     3721 ttgcctccgg ctcagtgcgc caggcggtcg aaaaagcgtc ctggctgcac ccggatgcgc
     3781 cagtagaagt cgaagtagag aatctggaag aacttgatga agccctgaaa gcaggagccg
     3841 atatcatcat gctggataac ttcgaaacag aacagatgcg cgaagccgtc aaacgcacca
     3901 acggcaaggc gctactggaa gtgtctggca acgtcactga caaaacactg cgtgaatttg
     3961 ccgaaacggg cgtggacttt atctccgtcg gtgcgctaac taaacacgta caagcactcg
     4021 acctttcaat gcgttttcgc taatagttga cagatttatc cgctccatcg cgggcggata
     4081 atctcccctt ccccaacttt cttcgtaacg cctcgcaaat ttcttgtctt tcgctgcaac
     4141 gaaataaaaa acctcggaat ctgctttccc gtttcgtttt ttgcccctcg cctgtgctga
     4201 tggcgtttgc caaagtagcg ccaactaaat caaggagcga aacagatgga caagcaacgc
     4261 ggttttacac ttatcgaact gatggtggtt attggcatca ttgccatttt aagcgccatt
     4321 ggtattcccg cttatcaaaa ctacctgcgc aaagccgcac tcaccgacat gctacaaacc
     4381 tttgtgcctt accgtaccgc cgtagagttg tgcgcgctgg aacatggtgg attagatacc
     4441 tgcgacggtg gcagcaatgg cattccctcg cctaccacca cccgctatgt ttcagccatg
     4501 agtgtggcaa agggcgtggt gtcgctgacc gggcaagaaa gtctcaatgg gctaagcgtc
     4561 gtcatgacac cgggttggga taacgcaaac ggcgtcaccg gctggacgcg caactgcaat
     4621 attcaaagtg acagcgcatt gcatcaagcc tgcgaagatg tcttccgctt tgatgacgcc
     4681 aactaaggag cggcaatgaa tattccacag ctcactgccc tgtgtctgcg ttatcatgga
     4741 gtcttgctgg atgccagcga agaggtggtt catgttgcgg tagtcgatgc accttcgcat
     4801 gagctactgg acgcattgca tttcgctacc accaaacgta ttgagatcac ctgctggacg
     4861 cgccaacaaa tggaaggtca cgccagtcgc acacaacaga cattgcccgt agctgttcag
     4921 gagaagcatc agcccaaagc agagttgcta actcgaacgt tacaatctgc gctggaacaa
     4981 cgcgcgtctg atattcatat cgaaccagcg gacaatgcct accgcatccg cttgcgtatc
     5041 gacggcgtat tgcatccttt accggatgtt tcaccggatg ccggagtcgc attaaccgcc
     5101 agattaaaag tgctgggaaa cctggatatt gcggaacatc gcctgccgca ggacgggcaa
     5161 ttcactgtcg aactggcagg aaacgccgtc tcatttcgta ttgcgacctt accatgtcgg
     5221 ggtggtgaaa aggtggtatt aaggttgtta cagcaggtga gccaggcact ggatgtcaac
     5281 acgcttggaa tgcagccgtt acaactggcg gactttgctc atgccttgca acaaccacag
     5341 ggactggtgc tggtaactgg ccctacaggc agcggcaaaa cggtcacgct ttatagtgcc
     5401 ctgcaaacgc tgaataccgc tgacattaat atttgtagcg tcgaagatcc ggttgagatc
     5461 cccatagccg gactaaacca gacgcaaatc catccgcgtg ccggactcac ctttcagggc
     5521 gtgttgcgtg cgttattgcg ccaggatcct gacgtcatca tgatcggaga gatccgcgat
     5581 ggcgaaacag cagagatcgc tattaaagcg gcgcaaactg gtcacctggt gttgtctacc
     5641 ctacacacta attccacctg cgaaacgctg gtacgtttac agcaaatggg ggtcgcccgc
     5701 tggatgctat catcggcgct tacgctggta atagcccagc gtctggtacg caaactttgc
     5761 ccacattgtc gccggcagca aggggagccc atccacattc cagacaatgt atggccatcg
     5821 ccgctgcccc actggcaggc acccggttgt gtacattgct accacggttt ttatggtcgt
     5881 acggccttat ttgaagttct gcccataacg ccggtcattc gtcagcttat ttccgctaat
     5941 accgacgttg aatcgctgga aacgcacgca cgacaggcgg gtatgcgtac gctttttgaa
     6001 aacggctgcc tggccgtgga gcaaggctta accacctttg aagagttaat ccgcgtactg
     6061 gggatgccgc atggcgagta agcaactctg gcgctggcat ggcataaccg gcgacggcaa
     6121 tgcgcaagat gggatgctat gggcagagag ccgtgctttg ctgctcatgg cactacagca
     6181 acagatggtt accccactta gcctgaagcg aatcgccatc aattctgcgc agtggcgagg
     6241 agataaaagc gcggaagtca ttcatcaact ggcgacgcta ctcaaagccg ggttaacgct
     6301 ttctgaaggg ctggcactgc tggcggaaca gcatcccagt aagcaatggc aagcgttgct
     6361 gcaatcgctg gcgcacgatc tcgaacaggg cattgctttt tccaatgcct tattaccctg
     6421 gtcagaggta tttccgccac tctatcaggc gatgatccgc acgggtgaac tgaccggtaa
     6481 gctggatgaa tgctgctttg aactggcgcg tcagcaaaaa gcccagcgtc agttgaccga
     6541 caaagtgaaa tcagcgttac gttatcccat catcatttta gcgatggcaa tcatggtggt
     6601 tgtggcaatg ctgcattttg ttctgccgga gtttgccgct atctataaga ccttcaacac
     6661 cccactaccg gcactaacgc aggggatcat gacgctggca gactttagtg gcgaatggag
     6721 ctggctgctg gtgttgttcg gctttctgct ggcgatagcc aataagttgc tgatgcgccg
     6781 acctacctgg cttatcgcgc ggcagaaatt gttgttacga atcccgatta tgggttcact
     6841 gatgagggga caaaaactca cgcagatctt tacgattctg gcgctgacac aaagtgcagg
     6901 cattactttt ttacagggcg tagagagcgt cagagaaaca atgcgctgcc cgtactgggt
     6961 gcaacttctg acacaaatcc agcacgatat cagtaacggt catcccatct ggctggcgct
     7021 aaaaaatgcc ggtgagttta gcccgctctg tttgcaatta gtgagaacgg gagaggcatc
     7081 cggctcgctg gatctcatgt tagacaacct cgcccatcat catcgggata acacaatggc
     7141 gctggcggat aacctcgcag ccttactgga accggcgttg ctgatcataa cgggaggaat
     7201 tatcggtacg ctggtggtgg cgatgtatct gccaattttc catttaggcg atgcgatgag
     7261 tgggatggga taacgctggc gtgttgctcc acgccagcgt tgggagatta caggttgttg
     7321 aagatgcggt tttcttgttc ctgcacccga ataaacgtgg tgcgcttggt cagttctttc
     7381 aggcgtgaag ccccaacgta tgtacaagct gaacgcaagc cgcccaaaat atcacgcgcg
     7441 gtattttcaa ccgggcctcg cagcggcagc ttaacggttt taccttctgc tgcgcgatat
     7501 tccgcaacgc cgccaacgtg acgtttcatc gcagactcgg agctcatgcc gtagaacagc
     7561 ataaatttct cgccgttctc ctcaacgatg cgaccgccgc tctcttcgtg gcccgccagc
     7621 atgccgccaa gcatgacgaa atcggcaccg ccgccaaagg ctttcgcgac atcgcccggc
     7681 gtggtgcagc caccgtcgct gacgatcatt ccgcccagac cgtgcgccgc atcggcacat
     7741 tcgattaccg cagaaagctg cggataaccg acgcctgttt tgacacgagt tgtacaaaca
     7801 gaacctgggc caatgccaac tttaacgata tcagcaccgg agaggataag ctcctcacac
     7861 atttcaccag tcactacgtt gcccgcacaa atggttttgg tcggccacgc ttcacgcgct
     7921 ttcgcaacga actgcacgaa gtgttcggaa taaccattcg ccacatcaat acaaacgaag
     7981 tttaatgccg ggttcaggtc gagaatttgt ttagtttttt cgaaatccgc atcagacgta
     8041 ccggtagaaa ccatcacatg tttcagcaca tcagcggaag aattgttgat aaacgcttgc
     8101 cactcttcga cagaatagtg tttatgcaca gcagtcaaaa tatcaaaaga agccagcgca
     8161 gaggccatag aaaatgtacc tacggtgtcc atatttgcgg cgataatcgg cacgccggac
     8221 cagctctgac ctgaatgttt gaaggtgaat tgacgttcca gttcaacatc ggaacggctt
     8281 ttaagagtgg agcgtttagg gcggatgaga acgtctttaa aacctaactt cagatcttct
     8341 tcaatacgca tgtgcggatt cctggggtta atggctataa gggtaagagc gcaactccag
     8401 tgacgctatc atacgcacta atcaatgcgg cgcaagactg cgaaattgtc tttttttacg
     8461 ctacaatccc ataaatttac ctggtaaata gtgagcgaac gtttcatcaa tttcgctttc
     8521 gtgcactcat ttttaacgtt ttgataatca tgatgaattc ccgggaatat aaattatgag
     8581 gtatatagtt gccttaacgg gaggcattgg cagtggcaag agtaccgttg ccaatgcgtt
     8641 tgctgatctc ggaattaacg tcattgatgc cgatattatt gcgcgtcagg tggttgaacc
     8701 aggtgcacct gcgctacatg ccattgctga tcactttggc gctaacatga ttgctgctga
     8761 tggaacattg cagcgccggg ccttgcgcga gcggatcttc gccaacccgg aagagaaaaa
     8821 ctggcttaac gccctgctgc atccgctgat tcagcaagag acgcaacacc agatccagca
     8881 agctacttcc ccctatgtac tgtgggttgt gccattgctg gtagaaaact cactgtataa
     8941 aaaagcgaat cgagtgctgg tggtggatgt cagcccagaa acgcaactta agcgcaccat
     9001 gcagcgcgat gatgtaactc gcgagcatgt cgaacaaatc cttgctgctc aggcaacgcg
     9061 cgaagcccgc cttgccgtgg cagatgacgt cattgataat aacggcgcac cggatgctat
     9121 cgcatcggat gttgcccgcc tgcacgcaca ctatttgcag cttgcgtcgc agtttctctc
     9181 acaggaaaaa ccgtaatgca gacccaggtc ctttttgaac atccactaaa tgaaaaaatg
     9241 cgtacatggc tgcgcattga gtttttgatt cagcaactca ctgttaattt acccatcgtt
     9301 gaccacgctg gcgcgctgca tttcttccgt aatgtcagtg aattactgga tgttttcgag
     9361 cgcggcgaag tccgcactga gctgttgaaa gaacttgacc ggcagcaacg taaactccag
     9421 acctggattg gcgtgcctgg cgtggaccag agccgtattg aagcattaat tcagcagtta
     9481 aaagcggcgg ggagcgtatt aatttccgcg ccgcgtatcg ggcaatttct gcgtgaagat
     9541 cgtttgattg ctctggtgcg tcagcgactg agcatcccag gcggctgttg cagctttgat
     9601 ttacctacat tgcacatttg gctgcatcta ccccaggcgc agcgcgacag ccaggtagaa
     9661 acctggattg ccagcctgaa cccgctcacc caggcactta ccatggtgct ggatttaatt
     9721 cgccagtcgg cccccttccg taaacaaacc agcctgaatg gtttttatca ggataacggt
     9781 ggcgatgccg acttgctgcg cctgaatctg tcgctcgatt cacagcttta tccgcaaatt
     9841 tccggacata agagccgttt tgccattcgt tttatgccgc tggacactga aaacggacag
     9901 gtaccggaac gtctggattt cgaactggcc tgttgctaag gagtgaaaat gtcagaaact
     9961 attacggtga attgcccaac ctgcgggaaa acggtggtgt ggggtgaaat cagcccgttt
    10021 cggccatttt gctccaaacg ttgtcagctg atcgacctcg gagaatgggc tgctgaagaa
    10081 aaacgaatcc ccagcagtgg cgatctttcc gaaagcgatg actggagcga agagccaaag
    10141 cagtgacatt tgtctgatgc cgca
//
LOCUS       DAZGVQ010000177        10145 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000177 DAZGVQ010000000
VERSION     DAZGVQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.178"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..526)
                     /gene="fliZ"
                     /locus_tag="ACHLAG_004394"
     CDS             complement(<1..526)
                     /gene="fliZ"
                     /locus_tag="ACHLAG_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFP0657515.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSS"
     gene            complement(572..1291)
                     /gene="fliA"
                     /locus_tag="ACHLAG_004395"
     CDS             complement(572..1291)
                     /gene="fliA"
                     /locus_tag="ACHLAG_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFP0657516.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1612..3264)
                     /gene="fliC"
                     /locus_tag="ACHLAG_004396"
     CDS             complement(1612..3264)
                     /gene="fliC"
                     /locus_tag="ACHLAG_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HFP0657517.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLSGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITSSNGDKLYIDTTGRLTKNGFSASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPNTITYSVTGAKVDQAAFDKAVSTSGNDVDFTTAGY
                     SVDGATGAVTKGVAPVYIDNNGALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            3530..4936
                     /gene="fliD"
                     /locus_tag="ACHLAG_004397"
     CDS             3530..4936
                     /gene="fliD"
                     /locus_tag="ACHLAG_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFP0657518.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTIS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            4961..5371
                     /gene="fliS"
                     /locus_tag="ACHLAG_004398"
     CDS             4961..5371
                     /gene="fliS"
                     /locus_tag="ACHLAG_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFP0657519.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            5371..5736
                     /gene="fliT"
                     /locus_tag="ACHLAG_004399"
     CDS             5371..5736
                     /gene="fliT"
                     /locus_tag="ACHLAG_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFP0657520.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEINPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNFS"
     gene            5814..7301
                     /gene="amyA"
                     /locus_tag="ACHLAG_004400"
     CDS             5814..7301
                     /gene="amyA"
                     /locus_tag="ACHLAG_004400"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP0657521.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSKFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTINLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(7335..7748)
                     /gene="yedD"
                     /locus_tag="ACHLAG_004401"
     CDS             complement(7335..7748)
                     /gene="yedD"
                     /locus_tag="ACHLAG_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFP0657522.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            7935..9140
                     /gene="yedE"
                     /locus_tag="ACHLAG_004402"
     CDS             7935..9140
                     /gene="yedE"
                     /locus_tag="ACHLAG_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFP0657523.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            9137..9370
                     /gene="yedF"
                     /locus_tag="ACHLAG_004403"
     CDS             9137..9370
                     /gene="yedF"
                     /locus_tag="ACHLAG_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFP0657524.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            9479..>10145
                     /locus_tag="ACHLAG_004404"
     CDS             9479..>10145
                     /locus_tag="ACHLAG_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFP0657525.1"
                     /translation="MCGRFAQSQTRVDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPV"
BASE COUNT         2489 a         2552 c         2582 g         2522 t
ORIGIN      
        1 tggaggattt ctgcacaagt ccggtacacg tttgacgctg atagtgttga tacttccgta
       61 atgcgatgcg gtaattgttg gtgctggtgg tgggcagcca cggggcgagg atttcatcaa
      121 ggaaaccgtc ctgcagcgga tcgagggaaa tattttgctc gtgtagatga ttccccaaac
      181 ggcgcagacg aacgacatat tcacgcaccg taccagggct catttcagtt tgctcaaaaa
      241 gaaattgctt aaagccgata atatcgaaaa aatcactctg cgttttgcaa tgtaaatcac
      301 cgcagaatcg gcacaatgcc gcccatgatt tttgttccac ttgccagcca ttttcatcaa
      361 gcagcatgtc caggcgggaa atctcgattt tattcactat tttgccgtcg cgaacgaggg
      421 taatgcggtc gagtaattta cggcaatgcg cgcaatgggt ctggctgtgt ttaaagtctt
      481 taagatagcg gcttaatggc cgtcttttca ggtgctgcac catcattaag aactcctggt
      541 agtcaaaatt aaagtgcggc atttactgac gttataactt acccagttta gtgcgtaacc
      601 gtttaatggc ctggctgtgt aactgactga cccgcgattc cccgacctcc agcaccgcgc
      661 caatctcttt gagattcagc tcttcctggt aatagagggt taataccagt ttttcgcgct
      721 ccggcaacgt ttcgatagct tccatcactc gctggcgcag attactgtcc agcagttgtt
      781 gtagcgggtt ttctcgctga tgatcatcag taaccagttc gatgctatcg ccgtgctctt
      841 cgcgccactc atcgtaggag aagagctggc tgttattggt gtcgagcaac atttggcgat
      901 aatcggcaat atcgatccct aaacgttccg ctacctcagt ttccgtggcg ttgcggccaa
      961 gttcctgctc cagttgccct attgcctgtg ccacttcacg cgcgttgcgt cgcacgctgc
     1021 gcggcaccca gtcacggctg cgaagttcat ccagcatagc gccacggata cgctgcactg
     1081 cgtaagttgt aaatgccgtt ccttgtaggg cgtcatagcg ttcgacggca ttaagtaacc
     1141 caatgccgcc cgcctgtagc agatcgtcaa gttccacgct cgcgggcagt cgaacctgca
     1201 ggcgcaatgc ttcgtgacgc accagcggga cataacgctg ccacagcgag tgtttatcca
     1261 ttacaccttc agcggtatag agtgaattca cgataaacag ccctgcgtta tatgagttat
     1321 cggcatgatt atccgtttct gcagggtttt taatcggacg attagtgggt gaaatgaggg
     1381 gttatttggg ggttaccggt aaattgcgga cagaaaaaaa ccccgccggt ggcggggaag
     1441 cacgttgctg acaaattacc attcatgttg ccggatgcgg cgtaaacgcc ttatccggcc
     1501 tacaaaaatg tgcaaattca ataaattgca gttcaccttg taggcctgat aagcgcagcg
     1561 catcaggcaa tttggcgttg ccgtcagtct cagttaatca ggttacgacg attaaccctg
     1621 cagcagagac agaacctgct gcggtacctg gttagctttt gccagcacgg agttaccggc
     1681 ctgctggatg atctgcgctt tcgacatgtt ggacacttcg gtcgcatagt cggcgtcctg
     1741 aatacgggac tgcgcttcgg acaggttggt agtggtgttg ttcaggttgg tgaccgcgga
     1801 atccagacgg ttttgcaccg caccgaggga ggagcggaat ttgtcgatgg agctgatggc
     1861 ttcgtccaga gctttcagtg gatcggcggt ggttgcagct ttagtttcag catctgtcgt
     1921 caatttaccg tcagcatctt tataaactgc cttaccgctg ccgttagtca ctgaaccatc
     1981 atcctgtaga taaaaatcta cagtatcaga tgtggtcaac gccccgttgt tatcaatata
     2041 aaccggagca acaccttttg ttacagcgcc agttgcgccg tcgacgctat aacctgcggt
     2101 agtgaaatca acatcgtttc cagaggttga tacagcttta tcgaaagctg cctgatcaac
     2161 ttttgcacct gttactgaat aagtaatagt gttcggcgta gtactattac cggtaaagga
     2221 gatagaggta ccgccaatgt caatggttgt cgctttggta ttattggctg caagtgtgga
     2281 cagactagcc tcagtcaaag aagcactaaa gccgtttttc gttaagcgcc cagttgtatc
     2341 aatgtaaagt ttatctccat tgcttgacgt aattttacca tcactgctga tctgaacatc
     2401 ctgtgcagaa ccatcgatag taatagtgcc cttagcggta tcaccaacgc ccggggtcaa
     2461 atatttctga acatcggcag ctgaagccgt tgtggtatca aaagagtaag acttacttgc
     2521 actgtcatac ttgtaattcg ttgcactagc cgccgttccg aagccgttat taatggttgc
     2581 ctgaacagta tcaccatcac taactccagc cagcaaatca gacgctttag cagcatcgta
     2641 acccgcactc acagtatatt tgttgcctac cacagttgca ttagcagcta ccaagtcagc
     2701 tttagatgca gcagtgttag caacagcccc gccaccattc acattaaacc cactcagccc
     2761 cagcgtatct gagtcaattt tcttcagatc aatcgtgata gtctggccgt cattcgcacc
     2821 aacctgaatt ttcatggaac cgtctttcgc cagtacgttc acgccgttga actgggtctg
     2881 gccagatacg cggtcaattt cgtccagacg ggatttgatt tcgtcctgaa tggagtccag
     2941 atccgaatcg gagttagtcc cggtagaagc ctgaaccgtc agttcacgga tacgctgtaa
     3001 gttgttgttg atttcggaca gcgcgccttc ggtggtctgc gcaacggaaa taccgtcgtt
     3061 ggcgttacgt gcagcctgag tcaggccttt aatgttagaa gtaaaacggt tagcaatcgc
     3121 ctgacctgcg gcgtcatcct tcgcgctgtt aatacgcaag ccagaagaca gacgctcgat
     3181 agaactcgac agcgcagact ggttcttgtt gatattattt tgagtgatca gcgagaggct
     3241 gttggtatta atgacttgtg ccatgattcg ttatcctata ttgcaagtcg ttgattacgt
     3301 attgggtttc cacccgtcgg ctcaatcgcc gtcaaccctg ttatcgtctg tcgtaaaaca
     3361 acctttagaa tttttttcac aaacagccat tttttgttag tcgccgaaat actcttttct
     3421 ctgcccctta ttcccgctat taaaaaaaac aattaaacgt aaactttgcg caattcaggc
     3481 cgataacccc ggtattcgtt ttacgtgtcg aaagataaaa ggaaatcgca tggcaagtat
     3541 ttcatcgctg ggagtcgggt caggtctgga tttaagttca atccttgata gcctcaccgc
     3601 cgcgcaaaaa gcgacgctaa cccccatttc aaatcagcaa tcgtcgttta ccgctaaact
     3661 tagcgcctac ggtacgctga aaagcgcgct gacgactttc cagaccgcca atactgcatt
     3721 gtctaaagcc gatctttttt ccgccaccag caccaccagc agcaccaccg cgttcagtgc
     3781 caccactgcg ggaaacgcta tcgccgggaa atacaccatc agcgtcaccc atctggcgca
     3841 ggcgcaaacc ctgaccacgc gcaccaccag agacgatacg aaaacggcga tcgccaccag
     3901 cgacagtaaa ctcaccattc aacaaggcgg cgacaaagat ccgattacca ttgatatcag
     3961 cgcggctaac tcgtcgttaa gcgggatccg tgatgccatc aacaacgcaa aagcgggcgt
     4021 aagtgcgagc atcattaacg tgggtaacgg tgaatatcgt ctgtcagtca catcaaatga
     4081 caccggcctt gataatgcga tgacactctc ggtcagcggt gatgatgcgc tacaaagttt
     4141 tatgggctat gacgccagtg ccagcagcaa cggtatggag gtctcggttg ccgcccagaa
     4201 tgcgcagctg acggtcaaca acgtcgccat cgagaacagc agcaacacca tcagcgacgc
     4261 gctggaaaac atcaccctga acctgaacga tgtcaccacg ggcaaccaga cgctaaccat
     4321 cactcaggac acctccaaag cgcaaacggc gattaaagat tgggtgaatg cctataactc
     4381 gctaatagat accttcagca gcctgaccaa atacaccgcc gtagatgcgg gagctgatag
     4441 ccagagttct agcaatggcg cactgctcgg cgactccacg ttgcggacga ttcagacgca
     4501 gttgaaatcg atgctgagta ataccatcag ttcttccaac tataaaacgc tggcgcagat
     4561 tggtatcacg accgatccca gcgatggcaa actggaactg gatgccgaca aactcaccgc
     4621 tgcactgaaa aaagatgcca gcggcgtagg tgcattgatt gttggcgatg gtaaaaaaac
     4681 cggcatcacg accaccatcg gcagcaacct gaccagttgg ctttcgacaa cgggcattat
     4741 taaagccgct accgatggcg ttagtaaaac cctgaataag ttaactaaag actacaacgc
     4801 cgccagcgat cgcattgatg cgcaggtcgc gcgctataaa gaacaattta cccaactgga
     4861 cgttttaatg acctcgttaa acagcacaag cagctactta acgcagcagt tcgaaaacaa
     4921 cagtaattcc aaataagcaa tattcatcgg gagacaggtc atgtacgcgg caaaaggcac
     4981 ccaggcctat gcacaaattg gcgtcgaaag cgccgtaatg agcgccagcc agcagcagct
     5041 ggtcaccatg ctatttgatg gagtgctgag cgcactggtt agagcgcgcc tgtttatgca
     5101 ggacaacaat cagcaaggca aaggcgtctc tttgtcaaaa gcgatcaaca tcattgagaa
     5161 cggactgcgg gtgagtcttg atgaagagag caaagacgaa ttaacccaaa acttgattgc
     5221 tctttatagc tatatggtca ggcgtttgct gcaagccaat ttacgcaacg atgtctccgc
     5281 agtcgaagaa gtggaagcat taatgcgcaa tattgccgat gcctggaaag agtcgttact
     5341 ctccccttct ttgattcagg acccagtctg atgaaccatg caccgcattt gtatttcgcc
     5401 tggcaacaac tcgtcgaaaa aagccagctc atgttacgcc tggcaacgga agaacaatgg
     5461 gacgaactca tcgccagcga aatggcgtat gtgaatgcgg tgcaggagat tgcacatctg
     5521 actgaagaga ttaacccatc caccacgatg caggagcagc tccgcccgat gctgcgcctg
     5581 attctcgaca acgaaagcaa ggtaaagcag ttattacaga ttcggatgga tgaactggcg
     5641 aaactggtcg gtcagtcatc ggtgcaaaaa tcggtgttaa gtgcctatgg cgatcagggc
     5701 ggctttgtgc tggctccgca agataacttc tcgtgaatct taacaagcta atggctcgcg
     5761 aataatctga ttacggctac gcttctaatg ttccccttga atggagtcga agaatgcgta
     5821 atcccacgct gttacaatgt tttcactggt attacccgga aggcggtaag ctctggcctg
     5881 aactggccga gcgcgccgac ggttttaatg atattggtat caatatggtc tggttgccgc
     5941 ccgcctataa aggcgcatcg ggcgggtatt cggtcggcta cgactcctat gatttatttg
     6001 atttaggcga gtttgatcag aaaggcagca tccccactaa atatggcgat aaagcacaac
     6061 tgctggccgc cattgatgct ctgaaacgta atgacattgc ggtgctgttg gacgtggtag
     6121 tcaaccacaa aatgggcgcg gatgaaaaag aagctattcg cgtgcagcgt gtaaatgctg
     6181 atgaccgtac gcaaattgac gaagaaatca ttgagtgtga aggctggacg cgttacacct
     6241 tccccgcgcg tgccgggcaa tactcgaagt ttatctggga tttcaaatgt tttagcggta
     6301 tcgaccatat cgaaaaccct gatgaagatg gcatatttaa aattgttaac gactacaccg
     6361 gcgaaggctg gaacgatcag gttgatgatg aattaggtaa tttcgattat ctgatgggcg
     6421 agaatatcga ttttcgcaat catgccgtga cggaagagat taaatactgg gcgcgctggg
     6481 tgatggaaca aacgcaatgc gacggttttc gtcttgatgc ggtcaaacat attccggcgt
     6541 ggttttataa agagtggatc gaacacgtac aggaagttgc gccaaagccg ctgtttattg
     6601 tggcggagta ctggtcgcat gaagtcgata aactgcaaac gtatattgat caggtggaag
     6661 gcaaaaccat gctgttcgat gcgccgctgc agatgaaatt ccatgaagca tcgcgcatgg
     6721 ggcgtgacta cgacatgacg cagattttca cgggtacatt agtggaagcc gatcctttcc
     6781 acgccgtgac gctcgttgcc aatcacgaca cccaaccgtt gcaagccctc gaagcgccgg
     6841 tcgaaccgtg gtttaaaccg ctggcatatg ccttaatttt gttgcgggaa aatggcgttc
     6901 cttcggtatt ctatccggac ctctacggtg cgcattacga agatgtcggc ggtgacgggc
     6961 aaacctatcc gatagatatg ccaataatcg aacagcttga tgagttaatt ctcgcccgtc
     7021 agcgtttcgc ccacggtgta cagacattat ttttcgacca tccgaactgc attgccttta
     7081 gccgcagtgg cactgacgaa tatcccggct gcgtggtggt catgtcgaac ggggatgatg
     7141 gcgaaaaaac cattaatctg ggagagaatt acggcaataa aacctggcgt gattttctgg
     7201 ggaaccggca agagagcgta gtaaccgacg aaaacggtga agcgaccttc ttttgcaacg
     7261 gcggcagcgt cagcgtgtgg gttatcgaag aggtgattta aattcatccc cggcggcaag
     7321 ccggggagat ttcattacgg cagtggcgtc ggtaacggcg ctttatccag cgcggcggca
     7381 cactctgccg tcgggcgatc cacacgctcc atcgtcatac catcgtactc aatggtgtta
     7441 ccgtcacgct caacctcgta cagctcacgt tttacggtaa cgttagtgag atcatctgac
     7501 atcaatgtca acttgcccgg cacggcaatc acccgctgcc actgacggca atccagggta
     7561 tcgccctctt tagtgacaat caggctgcct atcgcttccg gactgaccag cgcacgttgc
     7621 ggccctttgg tctgccagta gcccgccagc cagtccggcg cgggggtttt cacgacattg
     7681 ttatagtttt cgacttcggc acagcccgcc agcaacatga gcgcgcctgc aattgcgagt
     7741 tttttcatca ttcgtcctgc atgagaagaa aagaggattg tggcattaaa gccctgatgt
     7801 cgccagcctt tccaggttca gagagaaatt tgatctaact attatttttg tgaattatta
     7861 ccccgaaaat aaatttgcat gatgtatgat tcgcgactct tgtttcaaac cttctgagtt
     7921 cagaggctac attcatgtca tggcagcaat tcaaacacgc ctggttgatt aaattctggg
     7981 cacccatccc tgcggtcatc gcggcgggta ttctctccac ttactatttt ggcattactg
     8041 gcaccttttg ggctgtcacg ggtgaattta cccgttgggg cggccagctc ctgcagctgt
     8101 ttggcgtcca tgctgaagag tggggttatt ttaaaattat ccatctggaa ggatcgccat
     8161 taacccgcat cgacgggatg atgatcctcg gcatgtttgg cggctgcttt gccgcagcgc
     8221 tgtgggccaa caatgtcaaa ctgcgcatgc cacgcagccg tatccgcatt atgcaggcca
     8281 ttattggcgg tattatcgct ggttttggcg cgcgtctggc aatgggctgt aacctggcgg
     8341 cgttctttac cggtattcct cagttctcgc tgcatgcctg gttctttgcc atcgccactg
     8401 ccattggttc atggtttggc gcgcgcttta ccctgctgcc catcttccgt attcccgtga
     8461 aaatgcagaa agtttctgcc gcctcaccgc tgacgcaaaa accggatcag gcgcggcgtc
     8521 gttttcgtct cgggatgctg gtctttttcg gcatgctggg ctgggcgctg ctcacggcga
     8581 tgaaccaacc caaactgggg ctggcaatgc tgtttggcgt cggctttggt ttactgattg
     8641 aacgtgcgca aatctgcttt acttcagcgt tccgcgatat gtggatcacc ggacgtaccc
     8701 atatggcgaa agcaatcatt attggtatgg cggtgagtgc catcgggatc ttcagttacg
     8761 tacagttagg cgttgaaccc aaaatcatgt gggcgggacc aaacgcggta attggtggtt
     8821 tactgtttgg ttttggcatc gtgctggcag gcggctgcga aaccggctgg atgtaccgcg
     8881 cggtagaagg ccaggtgcac tactggtggg tcggtctggg caacgtgatc ggctcaacga
     8941 ttctggcgta ttactgggat gatttcgctc cggcgctggc caccgactgg gacaaaatca
     9001 acctgctgaa aacctttggc ccgatgggtg gcctgctggt gacatatttg ctgttgttta
     9061 ctgcgctgat gttgattatc ggctgggaaa aacgcttctt ccgccgtgcg gcaccgcaga
     9121 ctgctaagga gatcgcatga aaaatatcgt tcctgattac cgccttgata tggtgggtga
     9181 accctgccct tatccggcag tcgcaactct tgaggcgatg ccgcagttaa aaaaagggga
     9241 aatcctggaa gtggtgagcg actgtccaca gtcgatcaat aatattccac tggatgcgcg
     9301 taatcacggc tatacggtgc tggatattca gcaagacggg ccgaccattc gttatttaat
     9361 tcagaagtaa tccctcattc ccgctgtggc ccacagcggg aagatcgtta cctttgtatc
     9421 acatagattt tacccgcccc ctctcctatt cttttcagta tgatctcagg aggtttctat
     9481 gtgtggacgc tttgcccaat cccaaacgcg tgtagattac cttgcgcttc tcgcggaaga
     9541 tattgaacgc gatattccct atgatcccga acccattggc agatacaacg tcgcgccggg
     9601 aaccaaagtc cttctgctca gtgaacgtga cgaacacctt catctggatc cggttttctg
     9661 gggatatgct cccggatggt gggataaacc gccactgatt aacgcccgcg tagaaactgc
     9721 cgcaaccagt cgcatgttta aaccgctctg gcaacatggt cgggcaatct gttttgccga
     9781 tggctggttt gagtggaaaa aagaaggcga caaaaaacag ccttatttta tctatcgcgc
     9841 tgatggacaa cctgttttta tggccgcgat aggtagcaca ccgtttgagc gtggtgacga
     9901 agccgaagga tttttaattg tcactgctgc agcagaccag ggtctggtag acattcatga
     9961 ccgccgccca ctggtactgt cgccagaagc tgcgcgggaa tggatgcggc aagagattgg
    10021 cggtaaagaa gcatcagaaa ttgctaccaa cggctgtgtg ccagcaaacc agttcacctg
    10081 gcacccggta tcgcgcgcgg taggtaatgt caaaaaccag ggcgcggagt taattcaacc
    10141 tgttt
//
LOCUS       DAZGVQ010000178        10133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000178 DAZGVQ010000000
VERSION     DAZGVQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.179"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..609)
                     /locus_tag="ACHLAG_004405"
     CDS             complement(<1..609)
                     /locus_tag="ACHLAG_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFP0657526.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNE"
     gene            876..2375
                     /gene="ansP"
                     /locus_tag="ACHLAG_004406"
     CDS             876..2375
                     /gene="ansP"
                     /locus_tag="ACHLAG_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFP0657527.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVIFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2490..3551)
                     /locus_tag="ACHLAG_004407"
     CDS             complement(2490..3551)
                     /locus_tag="ACHLAG_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFP0657528.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3793..5895
                     /gene="pqqU"
                     /locus_tag="ACHLAG_004408"
     CDS             3793..5895
                     /gene="pqqU"
                     /locus_tag="ACHLAG_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFP0657529.1"
                     /translation="MKIVFVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTSVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSSRDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVISLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNLGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKQYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(5931..6596)
                     /gene="mcbR"
                     /locus_tag="ACHLAG_004409"
     CDS             complement(5931..6596)
                     /gene="mcbR"
                     /locus_tag="ACHLAG_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFP0657530.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6794..7831)
                     /gene="curA"
                     /locus_tag="ACHLAG_004410"
     CDS             complement(6794..7831)
                     /gene="curA"
                     /locus_tag="ACHLAG_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFP0657531.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8012..8530
                     /gene="mddA"
                     /locus_tag="ACHLAG_004411"
     CDS             8012..8530
                     /gene="mddA"
                     /locus_tag="ACHLAG_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFP0657532.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8527..8976
                     /locus_tag="ACHLAG_004412"
     CDS             8527..8976
                     /locus_tag="ACHLAG_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP0657533.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8977..9210)
                     /gene="ydcY"
                     /locus_tag="ACHLAG_004413"
     CDS             complement(8977..9210)
                     /gene="ydcY"
                     /locus_tag="ACHLAG_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFP0657534.1"
                     /translation="MSHLDEVIARVHAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9296..9469)
                     /gene="ortT"
                     /locus_tag="ACHLAG_004414"
     CDS             complement(9296..9469)
                     /gene="ortT"
                     /locus_tag="ACHLAG_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFP0657535.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9664..9759
                     /gene="yncL"
                     /locus_tag="ACHLAG_004415"
     CDS             9664..9759
                     /gene="yncL"
                     /locus_tag="ACHLAG_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFP0657536.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2453 a         2467 c         2468 g         2745 t
ORIGIN      
        1 ttcattcctt tttaatactt cttgtaattt cggtaactga catacagcat cagcaatcgc
       61 actgatattc gtggcattat cctgaaacca ttcatgccca ggcccccatg tgcgcatagt
      121 gcaaagataa cagtccacca gcgttagttg ctcaccaaac gcataaggtt cagcggtcaa
      181 ttgtgaattc agccagatat aaagcgattt acgatattca attacattct tctttaactg
      241 ttcaggtgcg tcaggagccc aacgctcagg gtaatcggcg aaagtgaatg ttggatagac
      301 attagcaacc agccagacca atagccgttg aaactgctga cgttcggcgc gcccaaccgg
      361 tggcgcaagg tccgggcgac gatcgagaac catcaatgca atcgccgctg tctccgtcat
      421 aatctcgtca ttttccagcg ccagtgtcgg aatctgacac aacgggttca gggttttcaa
      481 taactcgcgt gaagttccct cgtggtcaaa accactgaca tcaacgaact gataaggaat
      541 atcagccagt gttagcatca gctcactgat tgtagagccc catccgggta cgccatacac
      601 cttaatcata tgcccctcac aatcttttaa agattaagtg taggacagta cacaggttaa
      661 ccagcaaaag gatttgtcat tttttcgtca gttgtattaa tccccttcag gattgtaagg
      721 ggatgttttg catcgtttat cgataacggg taggatgctg cgccgatagt tttataccca
      781 taaagaataa tggtgataac tatcatcgcc aggatgaata aacattgttc atggcaactt
      841 atatgacttt ttcattaaag caatcaggga gagcaatgag taaacacgac accgacactt
      901 cagatcaaca cgccgcgaaa cgccgctggc ttaatgccca cgaagagggg tatcacaaag
      961 cgatgggcaa tcgccaggta cagatgatcg ccattggcgg cgcgattggc accggcttgt
     1021 ttttaggtgc aggagcccga ctgcaaatgg cgggtccagc actggcactg gtttatttaa
     1081 tttgtggctt gttttcgttt tttattctgc gtgcattggg tgagctggtg ctacaccgcc
     1141 cttccagtgg cagttttgtc tcttatgccc gtgagttttt gggtgagaaa gccgcttatg
     1201 ttgctggctg gatgtacttc atcaactggg cgatgacggg gattgttgat attaccgccg
     1261 tcgctctgta tatgcattac tggggtgcgt ttggcggcgt gccgcagtgg gtctttgcgc
     1321 tcgctgcact taccatcgtt ggcaccatga atatgatcgg tgtgaaatgg tttgcggaga
     1381 tggagttctg gtttgcgctt attaaagtgc tcgccattgt gatctttttg gtcgtgggta
     1441 cagtgttcct cggtagtggt cagccgctgg atggcaacac cactggcttt catttaatca
     1501 ccgataatgg cggcttcttc ccccacggtt tgctgcctgc gctggtgttg attcagggcg
     1561 tagtgtttgc ttttgcctcc attgaaatgg tgggtacagc tgccggagaa tgtaaagatc
     1621 cgcagaccat ggtgcctaaa gccattaaca gtgtgatttg gcgtattggc ctgttttacg
     1681 tcggctccgt ggtgttgctg gttatgctat tgccgtggag cgcgtatcag gcggggcaaa
     1741 gtccgttcgt gacgtttttc tctaaactgg gtgtgccata tatcggcagc attatgaata
     1801 ttgtggtgct gaccgctgcc ctctccagcc tgaactcagg tctgtactgc accggacgta
     1861 ttctgcgctc aatggcgatg ggcggttccg caccgagttt tatggcgaaa atgagtcgtc
     1921 agcatgtgcc gtatgccggg attctggcga cactagttgt gtatgtcgtc ggcgtattcc
     1981 tcaactatct ggtgccgtcg cgcgtatttg agattgtgtt gaacttcgcg tcgctgggaa
     2041 tcatcgcttc atgggcgttt atcatcgtgt gccagatgcg cctgcgtaaa gcaattaaac
     2101 aaggcaaagc agcggatgtc agttttaaac tgcctggcgc gcccttcact tcctggctga
     2161 cattactgtt tttactgagt gtcctagtgc tgatggcgtt cgattacccg aacgggactt
     2221 atactatcgc ggcgctgccg attatcggta ttctgctggt tataggctgg tttggtgtgc
     2281 gcaaacgcgt tgctgaaatt cacagcactg cgccagtcgt cgaggaagat gaagaaaaac
     2341 aggaaattgt gtttaagcct gaaacggcga gctaattgca aaattgcccg gctacgtgcc
     2401 gggcaaattt agattactga cgaaggtgtg ccgcatgcgg cgtgataaaa gtcaggcaag
     2461 tctctccgtt ttcaccctcg ccacatctat tacagcgcaa tacgaatcac atcgtccggc
     2521 tgggtagctt cctgctgttt agtggatttt tgtttcacac tgacatacag cgttttgcca
     2581 tcggcagaca gcgccaggct gtttggatga gtcggcgtat cgaacgtttt caccacttta
     2641 tagcttttcg cgtcaatcac actgacttta cctgcctgac gatgcgttac gtaggcttca
     2701 ttacgcgcgg ggttaaacag cacagccagt gattctggtg ccgcaacctt cgccagaata
     2761 ttgccattac gggtatcgac cactaacact tctgcggctt tagaatcggt gataaatgca
     2821 cgctgcctgg cggtatcaag gctaatgttg ataaagaagt gctctttgcc gtcatccagc
     2881 agctttttac ggctgaggat tttattgtcg gcggtgtcga tggtaatcaa ttcgccgtca
     2941 gcgttagtgg tgtaaagacg tttgcctttg ctatccagcg ccagaccggt actcatttta
     3001 ccggtgttct ggatggcggt tttcagtttg atattctcgc catcaacgac ccaaatcacg
     3061 ctctctttac caataccact gatgtaaacg gtgttcgtgg catcatcagc taccagctca
     3121 cgcggttgca gcgggcgcac ctcttccgtg cgcttacgat catccagcac cagacggcct
     3181 ttcacttcgc cagttttggc atctatcgcc gtgaccgcgc tgtttacggt gttaccaaac
     3241 cacaacgtct gagtcgtgtt attgatggtg gcaccaaacg gcttgagatc gttatggatc
     3301 gcttgggtca cttccagagt aaccgggtca agacgataaa caacaccgcc tttatccagt
     3361 ttgcggcttt gcgaagtggc gagccaaagc gcgttttctt gctggctata tgccatttcg
     3421 taggcacctt tacctaccgc tttacgcagc atttcttctg cggcctgcgt actgaatgat
     3481 gaagcaacaa gcaatgaacc taacagtaat gaaccacgca gacgcgatga aaacagatga
     3541 cgtaaatgca tgacgactcc ctttgatgaa ccgatatttt tgctgcttca caatgcttcg
     3601 ctaagcgtaa tcatcgttac gctcttgtta taaatgggaa tggtaatcat tattttctca
     3661 aatgtggagc atttcttgcc gttacgacta caattttctc aattacttaa cgtatcgtgc
     3721 cgtttcgttt tccataaatt tacaatttta ttaccatgac ggcgttttcc cattcttaac
     3781 ctagcttttt tcatgaagat tgttttcgtt cgacagaccg ttttgcccgc actgcttgtc
     3841 ctttcccccg ttgtttttgc cgctgatgaa cagaccatga ttgtcagtgc cgcgccgcag
     3901 gtggtttcag aactggatac ccctgcagca gtaagcgtgg tggatggcga ggagatgcgc
     3961 ctggcaacac cgcgcattaa cttgtccgaa tcactgacca gcgtgcctgg tttgcaggta
     4021 caaaaccggc agaactatgc gcaagattta cagctgtcga ttcgcggatt tggctcccgc
     4081 tccacttacg gtattcgcgg tattcgcctg tatgtggacg gtattcccgc caccatgccc
     4141 gacgggcaag ggcaaacatc caacatcgat ttaagcagtg tgcaaaatgt ggaagtgctg
     4201 cgtggcccct tttctgccct gtatggcaac gcgtctggcg gtgtaatgaa tgtcaccacc
     4261 cagaccggac aacagccacc aaccattgaa gccagtagtt actacggcag ttttggcagc
     4321 tggcgctatg ggctgaaagc aacgggcgca acgggagacg gcacacaacc cggcgatgtt
     4381 gattacaccg tctcaaccac gcgttttacg acccacggct atcgtgacca tagtggcgca
     4441 cagaaaaatt tagccaatgc caaactgggc gtacgcattg atgaagccag caaattaagc
     4501 ctgattttca atagtgtgga tatcaaagca gatgacccag gtgggctaac caaagcagaa
     4561 tggaaggcga atccacaaca agcgcctcgt gcagaacagt acgacacgcg aaaaaccatc
     4621 aagcaaactc aggctgggtt gcgctatgaa cgtagcctga gttcgcggga tgatatgagt
     4681 gtgatgatgt atgccggaga gcgagaaacg acccagtacc agtcaatacc catggcacca
     4741 caacttaacc cgtcacatgc gggcggcgtg attagcctgc aacgccatta ccagggaata
     4801 gacagccgct ggacacaccg tggtgaactg ggcgttccgg tcacgttcac taccggcctg
     4861 aactacgaaa acatgagtga aaaccgcaag ggctacaata acttccgcct gaatagcggc
     4921 atgccggagt acgggcaaaa aggtgagttg cgtcgcgacg aacgcaatct gatgtggaac
     4981 atcgatccct atttacagac gcagtggcag ctgagcgaaa aactgtcgct ggatgctggc
     5041 gtgcgctaca gctccgtgtg gtttgattcc aacgaccatt acgttactcc gggtaacggc
     5101 gatgacagcg gtgatgccag ttatcataaa tggctacctg ccggttcgtt aaaatatgca
     5161 atgaccgatg cctggaatat ctatctggca gccgggcgag gttttgaaac gccgacgatt
     5221 aatgagctgt cttatcgtgc tgatgggcaa agcggtatga acttaggttt aaaaccatcc
     5281 accaacgata caattgagat cggcagtaaa acgcgtattg gtgatgggct gcttagtctc
     5341 gcattgtttc agaccgacac tgatgatgaa attgttgtcg atagcagtag cggtgggcgt
     5401 acgacttaca aaaatgccgg aaagacccgt cgtcaaggcg ctgaactggc atgggatcaa
     5461 cgtttcgcag gagattttcg cgtaaacgcg tcctggacct ggcttgatgc gacctatcgc
     5521 agcaatgttt gcaatgaaca ggattgtaac ggtaatcgga tgccagggat cgcccgtaat
     5581 atgggctttg cgtcgatagg ttatgtaccg gaagatggtt ggtatgcagg cacggaagcg
     5641 cgttatatgg gcgatattat ggcagatgat gaaaatacgg caaaagcgcc gtcttatact
     5701 ctcgtcggct tattcaccgg gtataaatac aattaccaca atttaactgt ggatttattt
     5761 ggtcgtgtcg ataatttatt cgataaacaa tacgttggtt ctgtcattgt caatgagtca
     5821 aacgggcgat attacgaacc ttcgcccgga cgaaattatg gtgtcggcat gaatattgcg
     5881 tggagatttg agtaattacc ttgatgcccg gtatttgccg ggcatttact ttaacgattg
     5941 tattgctggt ataaaatagc aatattttgt tgcataattt cagcaagaca atgtctgcat
     6001 ccctctttgt cttttgcttt taacgcagcg agtaacagat gatagttttc tatatgctcc
     6061 cgtaattccg ctggattaat cgcttcatag agataatgta aaccaggccc catcctgacc
     6121 cacagttgct caatcatctc acacaggatg ggcatatttg agcgatgata aatcgctaag
     6181 cgaaatagcc tgtttacatt aatgatttgt tccatgtcgc ccttttcttg cgcctgctgt
     6241 aatttctcga gcaattcctg gagttccgca aggtcttgcg gggtgaggtt ttcaacagcc
     6301 agagcaactg ccattaattc cagttcgtag cggatccgat tgatttcgtc taattgacgt
     6361 ttccccactt ccggaactgt aaatgcttgt gcaggtgcga cagaaagcgc attcaccgaa
     6421 accagacgta ataatgcttc acgcacaggt gtaatactca tacctaattg ctccgccaga
     6481 tttttagtaa tcaggcgtgc gccaggtttt aatgcgccaa tactaagctg atgtttcagg
     6541 tcgttctcaa cctgcagagt caaactgaca tgtttcattt ttcccattcc aggcatttta
     6601 attccttcgc gtgttttctg tccaaccctg tctgcacggc agaaggagtt cacctggtgt
     6661 attaaagtga ataatcataa accacccacc tttgttgtta ttagcagcag aacaatgacg
     6721 tggttagtta attaaagctg aaaataagct ttttgcctgg atttttatgg cggcttacgc
     6781 cgccgttgaa actttaatca tcacccgcga cgcggatcac cactttgccg aagtttttac
     6841 ccttcagcag gccgataaac gtctgtggcg cattctctaa accatcagta atatcttcgt
     6901 ggtagtggat tttatcctct ttcacccatt gccccatctc cttctgaaac tcatggatgc
     6961 ggtgaccata atcctgagcg ataataaacc cttgcaagcg aatacgtttt ttcagcaccg
     7021 ttgccatcaa caggggtaaa cgatccggac cgggtggtag ctctgtagcg ttatagctgc
     7081 tcactaagcc acaaacggga atgcgcgcag atgtattaag taatggtagc actgcatcaa
     7141 ataccttacc gcccacgttt tcataataga tatcaatacc ttttgggcac gctttcacca
     7201 gttgttcggc aaaatcatcc gcgtggtgat caagacaaac atcgaagccc agcacctcga
     7261 tagcatggcg gcatttttcc gcgccaccgg caacccccac caccctgcaa cctttaagtt
     7321 tgccgatttg ccccaccgtt gctcccacag gacctgtcgc cgcagctacc accaacgttt
     7381 cgccctcttt aggctgaccg atatccagca gccccatata agcggtaaag cctggcatcc
     7441 ccagcacacc cagcgaccac gatggatttt gcggatgatc gccaagtttc accagatcat
     7501 caccactgga tatgtcatag tcctgccatc cactgtagcc cagcacccag tcgccaggct
     7561 gataatcagg atgattcgac tccacaacac ggctcaccgt gccgccgacc atcacgccgc
     7621 caatatcaac aggcggtgaa taagatggct catcgctcat acgtccacgc atatacgggt
     7681 ccagggacaa ataaactgtg cgcagtaaca cctgtccttc acccggtgtg gcgacatcat
     7741 cttcttcaag acggaaattc tccggaaccg gtgcgccatg tggacgcgag gccagaaccc
     7801 aacgtcgatt acgctgcttt tgttgcccca taatgctctc ctgtgctttt ggcatgaatc
     7861 catcaatagt agcaggctca cgggattggt tttgtttgca gccgtcacat cccctctgca
     7921 acacgcaata atttcatcga ggaatccgtt gcttcgggct gttttcgata gtattgataa
     7981 catcatcatc attattttat cgaggccaga catgtccatc cgttttgccc gcaaagccga
     8041 ttgtgctgcc attgcggaaa tttataacca cgccgtgttg tatacggcgg ctatctggaa
     8101 tgaccaaacg gttgatgcag ataaccgcat tgcctggttt gaagcgcgga ccatagcagg
     8161 ttatccggtg ctggtgagtg aagaagatgg cgtagtgacg ggatatgcct cgtttggcga
     8221 ctggcgtagt ttcgatggtt ttcgccatac cgtggaacat tcggtttatg tccatcccga
     8281 tcatcagggc aaaggtctgg ggcgtaaatt gttaagccga ttgattgatg aagcgcggga
     8341 ttgcgggaag catgtcatgg tcgccgggat cgaatcgcaa aatcaggcct cgctgcatct
     8401 gcaccagtcg ctgggatttg tcgtcaccgc acaaatgccg caggtgggca ctaaatttgg
     8461 gcgctggctg gatctgacat ttatgcagtt gcaactcgac gaacgcactg aaccggacgc
     8521 gattggatga atcagtcgct cacccttgct tttttgattg ccgccggaat tggtctggta
     8581 gtccagaaca cgctgatggt gcgcatcacc cagacctcct ctaccattct catcgccatg
     8641 ttgctgaact cactggtggg gattgtgctc tttgtttcta ttttatggtt taaacaaggc
     8701 atggcggggt ttggcgaact ggtgtccagc gtgcgctggt ggacacttat acccggcttg
     8761 ctgggatcgt tttttgtctt cgccagtatc agcggatatc agaatgtggg agcggcgaca
     8821 actattgcag tactggtcgc cagccagcta attggcggtc tgatgctgga tatcttccgc
     8881 agtcatggcg taccactacg ggcgttgttc ggcccaattt gtggcgcaat tttacttgtg
     8941 gttggcgcct ggttggtagc cagacgctca ttttaatcag aggatggtgc cgcctttggt
     9001 taactgttcg tggcgcgctt ccatatcttc tttatgcttg cgaccgtgat gagcaatagc
     9061 ggtgcgcaaa cgctgctgct gggtgtagcg atcttcccga cttaactccg cgtcatcgct
     9121 cagggcgatt aataattcgt tcatatgggc aatgacgctc tcttcaatcg cggcatgcac
     9181 gcgcgcgatg acttcatcca gatgcgacat actttctcct taaccacttg tgaaatttat
     9241 cgattcatcc aaatgatata agtctgcgtg gtgccgtcaa gccagtgagt aagatttaaa
     9301 acagtgaaaa caacagcgcc accgggaaac tcatcggcca tgttgccccc accagaaagg
     9361 cgcttaagaa acggatgcgt ttcttatcgt gagaaagaaa ccaggtgata agaaatgcga
     9421 ttgctgccat aaccgcataa aaaacaagca tatgttgata gagagacatg tacgggcgtc
     9481 cagccagatg aataaaacgc gcgcaaatat gcgtcatttt ttgacgtgca tcaagttttt
     9541 tcaccagttt gagaaaaaat catcaaacag caacttgccc ttatccatct tcagaatgag
     9601 tattattgcg gattttctta acccgtacta tacacatagg ggcaatgata aaaggtggca
     9661 aaaatgaatg tttccagtag aactgtagta ctgataaatg tctttgctgc tgttggtttg
     9721 tttactctta tctctatgag atttggctgg tttatttgat gtagtgattt tgcacgcagc
     9781 acgtactgtc agagaaatca actctctgac agtacgcaag atcttctcat actctccagg
     9841 tttacaacat tatccttaat atttacttct ttttgctggc atctacattg aaatgagtag
     9901 aaaacatccc cctactgtcg tctatacaaa cagcttactg attcaaacgg aatcttgttt
     9961 tcacattttt ctatcatgaa tactttatac gcgcgggagt ctataaatgc tttatacttt
    10021 caaggaataa cgtaaatata aaaagatggc gtaaagtata tgcatggcag gatgtttgca
    10081 acctccttga tgacgctgcc ccattgaaat tgcaaacccc gccctggtta tct
//
LOCUS       DAZGVQ010000179        10118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000179 DAZGVQ010000000
VERSION     DAZGVQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.180"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            16..90
                     /locus_tag="ACHLAG_004416"
                     /pseudo
     CDS             16..90
                     /locus_tag="ACHLAG_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            190..1068
                     /gene="glxR"
                     /locus_tag="ACHLAG_004417"
     CDS             190..1068
                     /gene="glxR"
                     /locus_tag="ACHLAG_004417"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP0657537.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            1238..2692
                     /gene="ybbW"
                     /locus_tag="ACHLAG_004418"
     CDS             1238..2692
                     /gene="ybbW"
                     /locus_tag="ACHLAG_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFP0657538.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            2752..4113
                     /gene="allB"
                     /locus_tag="ACHLAG_004419"
     CDS             2752..4113
                     /gene="allB"
                     /locus_tag="ACHLAG_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFP0657539.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            4170..5471
                     /gene="ybbY"
                     /locus_tag="ACHLAG_004420"
     CDS             4170..5471
                     /gene="ybbY"
                     /locus_tag="ACHLAG_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFP0657540.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTNGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            5493..6638
                     /gene="glxK"
                     /locus_tag="ACHLAG_004421"
     CDS             5493..6638
                     /gene="glxK"
                     /locus_tag="ACHLAG_004421"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFP0657541.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(6866..7651)
                     /gene="allE"
                     /locus_tag="ACHLAG_004422"
     CDS             complement(6866..7651)
                     /gene="allE"
                     /locus_tag="ACHLAG_004422"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFP0657542.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(7662..8897)
                     /gene="allC"
                     /locus_tag="ACHLAG_004423"
     CDS             complement(7662..8897)
                     /gene="allC"
                     /locus_tag="ACHLAG_004423"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFP0657543.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(8919..9968)
                     /gene="allD"
                     /locus_tag="ACHLAG_004424"
     CDS             complement(8919..9968)
                     /gene="allD"
                     /locus_tag="ACHLAG_004424"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFP0657544.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGFIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
BASE COUNT         2376 a         2391 c         2645 g         2706 t
ORIGIN      
        1 tgactacaac ggttgggttg ggttgggtgt gaatataaac cccaaaccac cacggaagcc
       61 ggtttacgct ggatggatcc gtaccgttaa aacgtaacgc tgttcaggca atgctttttt
      121 aggccgctaa gttggcaggg gatcgtgttg tctgaattca ggaaaagcga aatttaaaag
      181 aggttaatta tgaaactggg atttattggc ttaggcatta tgggtacacc gatggccatt
      241 aatctggcgc gtgccggtca tcaactgcat gtcacaacca ttggaccggt tgctgatgaa
      301 ttactgtcac tgggtgccgt cagtgttgaa actgcgcgcc aggtaacgga agcatcggac
      361 atcattttta ttatggtgcc agatacacca caggttgaag aagttctgtt cggtgaaaat
      421 ggttgtacca aagcctcgct gaagggcaaa accattgttg atatgagctc catttccccg
      481 attgaaacta agcgtttcgc tcgtcaggtg aatgaactgg gcggcgatta tctcgatgcg
      541 ccagtctctg gcggtgaaat cggtgcgcgt gaagggacgt tgtcgattat ggttggcggt
      601 gatgaagcgg tatttgaacg tgttaaaccg ctgtttgaac tgctcggtaa aaatatcacc
      661 ctcgtgggcg gtaacggcga cggtcaaacc tgcaaagtgg caaatcagat tattgtggcg
      721 ctcaatattg aagcggtttc tgaagccctg ctatttgctt caaaagccgg tgcggacccg
      781 gtacgtgtgc gccaggcgct gatgggcggc tttgcttcct cacgtattct ggaagttcat
      841 ggcgagcgta tgattaaacg cacctttaat ccgggcttca aaatcgctct gcaccagaaa
      901 gatctcaacc tggcactgca aagtgcgaaa gcacttgcgc tgaacctgcc aaacactgcg
      961 acctgccagg agttatttaa tacctgtgcg gcaaacggtg gcagccagtt ggatcactct
     1021 gcgttagtgc aggcgctgga attaatggct aaccataaac tggcctgata cccgcaataa
     1081 aaatggccga tatcagaaaa tgaatcggcc agcaatatta aaaaataaag cagccaaaga
     1141 tgttgcttca gtattaaaaa taatattttt tattttattt gttcctcata gctagattaa
     1201 aacaacgtta ttcgatacgt gaaattaaga gggatttatg gaacatcaga gaaagctatt
     1261 ccagcaacgc ggctatagcg aagatttatt gccgaaaacg caaagccagc ggacctggaa
     1321 aacatttaac tattttacct tatggatggg ttcggttcat aacgttccca attatgtgat
     1381 ggtcggcggc ttttttattc tcggcttgtc tacctttagt attatgctgg caattatcct
     1441 cagcgccttt ttcattgccg cggtaatggt attaaacggt gctgcgggca gtaaatacgg
     1501 cgtaccgttt gccatgatcc tgcgtgcttc ttacggcgta cgtggcgcac tgtttcccgg
     1561 attattaaga ggtggtattg ctgccattat gtggtttggc ctgcaatgtt acgccggatc
     1621 actggcctgc ttgatcctga ttggcaaaat ctggccggga tttttaactc tcggtggtga
     1681 ttttaccttg ttaggccttt cgctaccggg cttaattact ttcttactct tctggctggt
     1741 taacgttggt attggttttg gcggtggcaa agttttaaat aaattcactg ccattcttaa
     1801 cccgtgcatc tatatcgttt tcggcggcat ggcgatttgg gcgatttcac tggtcgggat
     1861 cggtccaatc tttgactaca ttccgagcgg tattcagaaa gcagaaaaca gtggattctt
     1921 gttcctggtg gtgattaacg cggtagttgc ggtctgggcg gcaccggcgg tgagcgcatc
     1981 cgactttacg caaaacgccc actcgtttcg tgagcaagcg ctggggcaaa cgctgggttt
     2041 agttgtggcc tatattctgt ttgcggtcgc tggggtatgt attattgccg gagccagtat
     2101 tcactacggc gctgacacct ggaacgtgct ggatattgtt cagcgttggg acagcctgtt
     2161 tgcctcgttc tttgcggtac tggttattct gatgacgact atttccacta acgccaccgg
     2221 taatattatt ccggccggtt atcagattgc tgccattgca ccgacaaaac tgacctataa
     2281 aaacggcgta ctgattgcca gtattatcag cctgctgatc tgcccgtgga aattaatgga
     2341 aaatcaggac agtatttatc tgttcctcga tatcatcggc ggaatgctgg gtccggtaat
     2401 tggtgtcatg atggcacatt attttgtggt gatgcgtggg caaattaatc ttgatgaact
     2461 gtacaccgca cctggcgatt ataaatatta cgataacggt tttaacctca ccgcgttttc
     2521 agtaactctg gtggccgtta ttttatctct tggcggtaag tttattccct ttatggaacc
     2581 gttatcgcgt gtttcatggt ttgtcggcgt catcgtcgcc tttgcggcct acgccttatt
     2641 aaagaaacgt acaacagcag aaaaaacagg agagcaaaaa accataggtt aattaatccc
     2701 gatattgaac attgagttaa aaaccaatct gtattttaca aggagtttgt tatgtctttt
     2761 gatttaatca ttaaaaacgg caccgttatt ttagaaaacg aagctcgcgt tgtagatatc
     2821 gccgttaaag gcgggaaaat tgctgctatc ggtcaggatc tgggcgatgc aaaagaagtt
     2881 atggatgcgt ctggtctggt ggtttcgccg ggcatggttg atgcgcacac ccatatttct
     2941 gaaccgggtc gtagccactg ggaaggttat gaaaccggta ctcgcgcagc ggcaaaaggt
     3001 ggtatcacca ccatgatcga aatgccgctc aaccagctgc ctgcaacggt tgaccgcgct
     3061 tcaattgaac tgaagttcga tgccgctaaa ggcaagctga ctatcgatgc ggcacaactc
     3121 ggtggcctgg tgtcttacaa catcgaccgt ctgcatgagc tggatgaagt gggcgttgtc
     3181 ggcttcaaat gcttcgttgc gacctgtggc gatcgcggta tcgacaacga cttccgtgat
     3241 gtaaacgact ggcagttctt caaaggtgcg cagaagctgg gcgaactggg gcagccggtg
     3301 ctggtgcact gcgaaaacgc gctgatctgt gacgcactgg gcgaagaagc gaaacgtgaa
     3361 ggtcgcgtaa ctgcccatga ctatgtggct tcgcgtccgg tatttaccga agtggaagcg
     3421 attcgccgcg tgctgtatct ggcgaaagtt gccggttgcc gtctgcacgt ttgccatatc
     3481 agcagcccgg aaggcgttga agaagtgact cgtgcacgtc aggaaggtca ggacgttact
     3541 tgtgaatcct gcccgcatta ctttgtactg gataccgatc agttcgaaga aatcggcact
     3601 ctggcgaagt gttcaccgcc gatccgcgat ctggaaaacc agaaaggcat gtgggaaaaa
     3661 ctgtttaacg gtgaaatcga ctgcctggtt tccgaccact caccatgccc tccggaaatg
     3721 aaagccggca acatcatgga agcatggggc ggtattgccg gtctgcaaaa ctgtatggac
     3781 gtgatgttcg atgaagcggt acagaaacgc ggaatgtctc tgccaatgtt cggcaaatta
     3841 atggcgacta acgcagcaga tattttcggt ctgcagcaaa aaggccgtat cgccccagga
     3901 aaagatgccg acttcgtctt cattcagccg aatagcagct atgttcttac caatgacgat
     3961 ctggaatatc gccacaaagt cagcccgtat gttggccgta ccattggcgc gcgtatcacg
     4021 aaaaccatct tacgtggtga tgtgatttac gacattgaac agggcttccc tgttgcgccg
     4081 aaaggtcaat ttatccttaa acatcagcag taatctggcc cctgcaatgc ccgtccttgc
     4141 ggcgggcatt ctccggttaa ggtgtgttta tgttcaattt tgcagtcagc cgcgaaagcc
     4201 tgttatcagg atttcagtgg tttttcttta ttttttgcaa cacggttgtg gttcctccta
     4261 cgctactttc tgcttttcag ttgccgcaaa gtagcctgct tacgctcacg caatatgctt
     4321 ttcttgctac cgcactggcc tgcttcgctc aggcgttctg cggtcatcgt cgcgctatta
     4381 tggaagggcc gggtggcctg tggtggggaa ccatccttac tatcaccctt ggcgaagcat
     4441 cgcgcgggac accgatcaac gatatcgcta ccagtctggc ggtggggatt gcactctccg
     4501 gcgtgctgac gatgttgatt ggttttagcg gattaggcca tcgcctggca cggttattta
     4561 cgccgtcggt gatggtcttg tttatgttga tgctgggcgc gcagctgacc actatctttt
     4621 tcaaaggtat gctcgggctg ccgtttggca tagccgaccc aaattttaaa attcagttac
     4681 caccgttcgc gctctcggtg gcggtgatgt gcctggtact ggcgatgatt atctttctgc
     4741 cgcaacgttt tgcccgttat ggcctgctgg tcggtacgat aaccggctgg ttgttgtggt
     4801 acttttgctt tccttcttcg cattcgctct ccggtgagtt gcactggcag tggttcccgc
     4861 tcggcagtgg cggtgctttg tcgccgggaa ttattctgac ggcggtgatt acaggtctgg
     4921 tgaatatcag caataccaac ggtgcgattc ggggcacgga tgttttttat ccgcagcagg
     4981 gcgcggggaa tacgcgttat cgtcgtagct ttgtggcgac cggatttatg acgctgataa
     5041 ccgtaccgct ggcggtaatt ccattttcac cgtttgtttc atccattggt ttattaaccc
     5101 agactggcga ttacacgcgg cgttcgttta tttatggcag cgttatttgc ctgctggtgg
     5161 cgctggttcc ggcactcaca cgactgtttt gcagtatccc tttacccgtg agtagcgcgg
     5221 tcatgctggt ttcttatctg cctttactct tttccgcgct ggtgtttagc cagcaaataa
     5281 cctttaccgc tcgcaatatt tatcgactcg cattgccgtt atttgtcggc atatttttaa
     5341 tggcattacc gcctgtgtat ctgcaagacc ttccattaac gcttcgtcct ctgctcagta
     5401 acggcttatt ggtcgggatt ttactggctg ttcttatgga taaccttatt ccgtgggaac
     5461 gtatcgaata atttgttgaa aaaggattga taatgaagat tgtcattgcg ccagactctt
     5521 ttaaagagag cttaagtgca gaaaaatgtt gtcaggcaat taaagccggg ttttcgaccc
     5581 tctttcccga tgcgaactat atctgtttgc cgatagcgga tggcggcgaa ggaacggtgg
     5641 atgcgatggt cgccgcgacg ggcggcaaca tcgtgacgct tgaagtctgc gggccgatgg
     5701 gcgaaaaagt gaatgctttt tatggcctta ccggcgacgg gaaaacggcg gtgattgaga
     5761 tggcggcagc aagtggcctg atgctggtcg cgcctgaaaa gcgtaatccg ttgctggcct
     5821 ccagttttgg tacgggggag ttaattcgtc atgcgctgga taacggcatt cgccatatta
     5881 ttctcggcat tggcggcagt gcgacggtcg acggtggtat gggtatggcg caggcgctcg
     5941 gtgtgcgttt ccttgatgcc gacggtcagg tgctggtggc taacggcggt aatttagcgc
     6001 gcgtggcaag cattgagatg gatgaatgcg atccgcgtct ggcgaattgc catattgaag
     6061 tagcatgtga cgttgataac ccgctggtag gggcacgcgg cgcggcggcg gtgtttggcc
     6121 cgcaaaaagg ggcaacgccg gaaatggtcg aagaactgga acaggggctg caaaattacg
     6181 cccgtgtttt acaacagcaa acggaaatta atgtctgcca gatggcgggc ggcggcgctg
     6241 cgggcggtat gggtattgcg gcggcggtat ttctcaatgc ggatattaaa ccgggtattg
     6301 agattgtgtt gaatgcggtc aatcttgcgc aggcagtgca gggtgcagca ctggtgatca
     6361 ccggggaagg gcgcatcgac tcgcaaacgg caggcggtaa agcgccgctg ggtgtggcgt
     6421 cggtggcgaa gcagtttaat gtaccggtga ttgggattgc tggcgtattg ggtgatggcg
     6481 tggaagtggt gcaccagtac ggcattgatg cggtattcag cattttgcca cgtctggcac
     6541 ctttagccga agtactcgcc agcggtgaaa ccaatctctt caacagcgca cgaaatattg
     6601 cctgcgccat taaaataggt cagggaatta aaaactgacc cttaccttta aagcggatgc
     6661 gatttatatc gcataagagt gcagtactca tgccggatgc ggcatgagta ctatatcctt
     6721 cctgaaaatc gcgcaaattc tatatattgc agagatcatg taggcctgat aagcgaagcg
     6781 catcaggcaa tgttacaaaa aaagccacgg tataaaccgt ggcaaaatcc aacatagcta
     6841 aaaataatca ggcgagtggt atgacttaaa tctctacgtc gcggttgcaa tctttcgagt
     6901 aaatatagct gaacgcttca ccacgcccta caccataacc agcctgtaaa gaataagcgc
     6961 ccataaagat gtaatcgcct tttttcaccg ggatccagtt attgtcgagg ttataaaccc
     7021 cctgaccgga aagaatatag gcaccgtgtt cctgaacatg tgtttcgata taaccgtggc
     7081 tggcacctgg tgcaaaagag aggatatgca tgttcatatc aaaacctaac tctttgggca
     7141 gaaaatccag caggatcaca tcatccatac cttcatagtg aatgcgttcc agttcgctgg
     7201 cattgccaga aaccagccac ggtgcatggc cttctaccgg aacatagcga cgcttatata
     7261 aaaagatttg gctgtcttcg gcctgggcgt taacaaacgt cattaaggag cctggtgggc
     7321 aataaagata gccaccttcg cttaaggcaa atgttttgcc ttcggctttg gcagtgatat
     7381 ttccagagat cacatacagg aacgtttcaa tgccttcgcc accgaagccc tgttggttgc
     7441 caccgttttg atgcagtgtg accagataat caacaaaaga ggcacccagc tttggcgtgg
     7501 agaggattgt cgcgtcacaa ttttcaaagc ccggaataat attttttacc agaccatccg
     7561 gggttaacag tgcgaaatta ccgtgtttaa caatcgcacg gttagccagt aaatcatcgc
     7621 ggtaaccggt gacgttattt aaatatccca tttatgactc cttatttctg ccaggcaagt
     7681 tgataaagca tgagtgccaa cgttttgacc ccttcggcaa ggtcggtaat attggtgcgt
     7741 tccgccgggt tatggctgat cccgttgatg ctgggaatga aaatcatgca ggttggtacg
     7801 cgaggcgcga aaatttgcgc gtcgtgcccg gcaccactgt gcatcacccg gtaattcagt
     7861 ttttcacttt cacacaattc tgtcagggtg gcgaccagct ccttattcat cggcacgggt
     7921 tcttcgtcca tccataaatc gatatcaata ccaatgtcca tttcatcgca aatcgcccgc
     7981 atgtcgtttt ctaactgttg ggtgaaatcg cgcagcacgg cagcgtcggt atgacgacaa
     8041 tcaatggtga acgtggtttt acccggcacc acatttaccg tattcgggcg cggctctact
     8101 ttgccaaagg tcagaaccag cggatcgccc atccttttcg ctttttcgac cgactgatgg
     8161 caaatgcgac tgaaagcgta aactgtatca cgacgataac ccatcggcgt ggtgcctgca
     8221 tggtttgatt caccgttcag cgttaccgta taacgacgct gcccgacaat tgcattcacc
     8281 acgccaattg attgcccatt actttccagc acacagccct gttcaatatg cagttcaaca
     8341 aaggctttaa tatcctgacg cggagttagt ggggcgttcg gaagagtaaa tccgcaagcc
     8401 ttcatcgcat cgacaaaact atttcctttg gcatcacaga tattccgcac gtcgtcagga
     8461 ttcgccagcc caaagatatt tttactgccc cagaagacat acgggaagcg gctgccttct
     8521 tcttctgcca tcgccaccac ttcgaccgta cgtagcggcg cgccgtattg cgttttcagc
     8581 cagtcaattg ccagccacgc cgccagcgcg ccgaattgcc cgtcgaggtt accgccgtta
     8641 accacggtat cgatatgcga accgctcaga atcacttcct gtggatattc ggtaccattc
     8701 aggcgaccgt ataaattccc tacttcatcg aaacgtgttt ccagcccact tgctgccatt
     8761 ctttttttaa attgctgctg ggtttccagc cattccggcg aataaagtaa acgggtcatc
     8821 ccacccgttg ggtcagcgcc aaaagaggaa agccagggca gcgtttcttc tatggcttga
     8881 cgaaaatgtg taatcataag aaagtcctgt ctcaataatt attgcgcaaa gggatttttc
     8941 gtttcgtatg acgtgttata aagcgcgtcg gaaattaaat actggtaaat atcatcaaca
     9001 atttcgatgc cttcgacggc ggctttgcgt tgtttaatat cctgatcctg tccgggataa
     9061 taaacttggt taaaaccggg cgcgggggta atggcattta attcgcgcat ggtctggcta
     9121 agatgttgac ggaataattc gctggaggag aaaaagttcg gattaataac tatatgtaat
     9181 tgccccaaat tacgccctgc gtgtaaatcg tcatacatcg aactaacctg tcgcccgaac
     9241 ggtaagccga gtaagacgcc tgagaggacg tcaatcatca tcatcaggcc ataccctttt
     9301 ggcccggcgg cggggagcag agcatgtacc gcgaacggat cggttgttgg tgcaccgttt
     9361 ttatcgaccg cccaggtatc cgggatagac atattacgcg agcgggcgtc gagcactttt
     9421 ccccatgcct gtacggtagt cgccatatca aaggtaagga tctcgtcgcc ttctcccggc
     9481 gcggcaaagg ccagggggtt agtaccgtag taaatttccg cgccgccaaa cggcaccacc
     9541 attggatcgg actggcacat cgaaatgcca atgaatccgg cgcgggctgc ctgctgcaca
     9601 aaataagaga ttgcgccgct gtgacccatc cggctgatac cgaccaccgc aacgccattt
     9661 tgctgggcgg ttttgatggc atgttccata cccattttcg ccgcgacctg tccggcggca
     9721 ttgtcggcat gtaaaattgc cgagcacggc ccggtttcct caagacgaaa ctccggttcg
     9781 cggttggtgc cgccttttga aatgcgttcc gcgtagtatt ccacgcgcac cgcgccatga
     9841 gagtggatcc ctctggcatc ggcgtaaacc aatacttcag ccacggttgc agcgtgctca
     9901 cgttttaacc cagcctggca gagtttattc tcaattagct ggtggagtgt ttcccgactg
     9961 attttcatct gtcttccttt ttaacgacgg tgtgaagcat gactgcaatt aacatacagg
    10021 gaaaatatct ggattatgtg atccagacag gcaaaaaaat atagttagaa tttatttgat
    10081 aatccgctcg cttttaacct ggtttttaaa acaaccac
//
LOCUS       DAZGVQ010000180        10105 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000180 DAZGVQ010000000
VERSION     DAZGVQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.181"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(422..1615)
                     /locus_tag="ACHLAG_004425"
     CDS             complement(422..1615)
                     /locus_tag="ACHLAG_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0657545.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRAIK"
     gene            1751..3475
                     /gene="iucA"
                     /locus_tag="ACHLAG_004426"
     CDS             1751..3475
                     /gene="iucA"
                     /locus_tag="ACHLAG_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFP0657546.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHAHTQQAIDARHDWTALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3476..4423
                     /gene="iucB"
                     /locus_tag="ACHLAG_004427"
     CDS             3476..4423
                     /gene="iucB"
                     /locus_tag="ACHLAG_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFP0657547.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWCEEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4423..6165
                     /gene="iucC"
                     /locus_tag="ACHLAG_004428"
     CDS             4423..6165
                     /gene="iucC"
                     /locus_tag="ACHLAG_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFP0657548.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDQDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6162..7499
                     /gene="iucD"
                     /locus_tag="ACHLAG_004429"
     CDS             6162..7499
                     /gene="iucD"
                     /locus_tag="ACHLAG_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFP0657549.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDRGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTTNIQE"
     gene            7505..9700
                     /gene="iutA"
                     /locus_tag="ACHLAG_004430"
     CDS             7505..9700
                     /gene="iutA"
                     /locus_tag="ACHLAG_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFP0657550.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLRQELVGQVYYRDESLRFYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYMQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYISWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWLLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(9872..>10105)
                     /locus_tag="ACHLAG_004431"
     CDS             complement(9872..>10105)
                     /locus_tag="ACHLAG_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFP0657551.1"
                     /translation="STGDSYDNAMAESINGLYKAEVIHRKSWKNRTEVELATLTWVDW
                     YNNRRLLGRLGHTPPAEAEKAYYASIGNNDLAA"
BASE COUNT         2372 a         2644 c         2680 g         2409 t
ORIGIN      
        1 gcgcagttca cgattttctc gctccagctc tttcaggtat caacgccact ctgagagagt
       61 tttatgcatc ccgtattggg cactatgatg ccggaaatct gcgaggctcg acacctctct
      121 gattttcccc tttatttatc acggtgcccc agatctaatg ttggggatgc gttcatctca
      181 ttcccaatga gtaatcgcac tgttctcatt gtctgcaggc atccggacag agccatgtga
      241 tgttgcactc acaaccagca tgacttcaat gcaacttaag tgacaccggc cggttttata
      301 ccgaacatac cctgtaccat gcggtgcatt tttgccagtg ttactgcctg acatgctttc
      361 atcagacgca ggttctctct catatcctct gatttactcc ggtaaggtga tgatcatatc
      421 tttatttgat agcacggtaa tgccagataa atacagacac aatggtaaat aatatagttg
      481 caaataatgc cgccataatc agcgcgattg cgacacctgt tttcgatacc agcggcacca
      541 gcaaagcgca cagtccatag ccaagtgtgt gactggtagc gagccagccc gcgcctgcgc
      601 catcagccag cttatcattc agtagaagtt gataagcggg tgttgccaga gcagctccaa
      661 atgacagcac tgcacagccc gggtaaaaca accatagctg ttcggagagc atgatagcca
      721 gaccaccact catcaacact ccggcactca ggagcagggc caccggagtc aggcgctgcg
      781 gacggagtac cccgaactgc gcgataagcg cagctactgc ggacagaccc aacagccagg
      841 ccacctgttg gctaatggcg gtggtatccg tggcgaactg gcgagtaagg gcaggcgaaa
      901 gtccaagctg catcatgctc accgccgcag ccagtaatat tgcgcaaagc agataaggca
      961 gacaatcccg cttcagactg acgctcttgc gctccggtgt gggctgtggt ggtgtgccgg
     1021 gcaaccgcag cagcatcagc agcgccagca ctggggctgc catcagcagc cccagtggcg
     1081 ccagaggatg aatggccagc attgctgccg cgcacagcgg cccgaatagc ctgccgcaac
     1141 tcaggccgga gctgatggtt gccagagcgg ccatgcgatt cccttctcct gctctctgca
     1201 atgcccagac ctgacaggct ggcaccattg ccgacacggt cagcccgtag gcgatccggg
     1261 cgatgattaa tattcccagt cccaccgctg ttgttatggc gctggtagcc atcagcacgc
     1321 ttcccagccc tagcagggta aagcttatgc catatcctcc cagagcccac aacaccacag
     1381 gtttactgcc agtacgggaa atttgctttc cccaccacgg ggacgatggc agaaacagca
     1441 ttgagccaag catcagcagg gcagcccata cagacagact cagatttgtc tgtatgacca
     1501 gaacagggag cacaaccagc agaccgttct gcccgatacc gagaagcccg gcactgaacg
     1561 caagtggcca gcaggacagt ggtttttggg gcgtatcttc gatcccaggt gacattttaa
     1621 tgtttcaact ccatgtatta attgtgttta tttgtaaaat taattcatct gacaataaca
     1681 tttcttattg ataatgagaa tcattattga cataattgtt attattttac tgtgtgggag
     1741 ctgtttgact atgaccctgc cctctgaaaa accagccaca gatgtggctg cgcagtgctt
     1801 cctgaatgca ctgattcgtg aaaccacaga ctggaaactg acagaatacc cgccagacga
     1861 attgatcatc ccgctggatg agcagaaatc gctccatttc agagtggctt atttctcccc
     1921 aacccagcat caccgctttg catttcctgc acgtctggtc acggcatcag gcagttatcc
     1981 tgtcgacttt accactctct cccggcttat tattgataag ctacggcatc aactgtttct
     2041 gcccgttccc ctctgcgaaa ctttccacca gcgcgtgctg gaaagccacg cccatacgca
     2101 acaggcaatt gatgcccgtc atgactggac cgccctgcgt gaaaaagcgt tgaattttgg
     2161 cgaagctgag caggcgctgc tgacaggaca cgctttccac cctgcgccta agtctcatga
     2221 accgtttaac cggcgggagg ctgaacgcta cctgcctgat atggcacccc acttcccact
     2281 gcggtggttt tcggtggata aaacgcaaat cgccggtgaa agtttgcatc tcaaccttca
     2341 acagcggctg acgcgatttg ccgcagagaa tgcgcctcag ttactcaacg aattaagtga
     2401 caaccaatgg ctgttcccgt tgcacccgtg gcagggggaa tatcttttgc agcaggggtg
     2461 gtgccaggca cttgttgcta aagggctgat taaagactta ggtgaggccg gcacgtcgtg
     2521 gctgccgacc acctcttccc gttccctcta ctgtgccacc agccgcgata tgatcaagtt
     2581 ctccctgagc gtacggctga ccaactccat ccgtactctg tccgtgaaag aagtgaagcg
     2641 tgggatgcgc ctggcacgcc tggctcaaac cgacggctgg cagatgctac aggtccgctt
     2701 cccgactttc cgggtaatgc aggaggatgg ctgggccggg ctgctcgatc ttaacggcaa
     2761 catcatgcag gaaagtctgt ttgccctgcg tgaaaatctg ctggtggatc agccgaaaag
     2821 ccagaccaac gtactggtct ccctgactca ggccgcacct gatggcggtg attcgctgct
     2881 ggtttcggcg gtaaaacgcc tgagcgatcg cctcggtatc actgtgcaac aggccgccca
     2941 tgcatgggtc gatgcatact gtcagcaagt gctaaagccg ctgtttacgg ctgaagcgga
     3001 ttacggcctg gtgctgctgg cgcatcagca aaatattctt gtccagatgc ttggggatct
     3061 gccggtcgga tttatttacc gtgactgtca gggcagcgct tttatgcctc acgcgacaga
     3121 ctggctcgat tctattggcg aggcgcaggc ggaaaatatt ttcacccatg agcagttgct
     3181 gcgctatttc ccttattacc tgctggttaa ctccactttt gctgtgaccg ctgcgctggg
     3241 ggctgccggg ctggacagcg aatcgaatct gatggctcgt gtacgagcat cgctggctga
     3301 agtgcgtgat caggtgactc ataaaacctg tctcaactac gtgctggaaa gtccgtactg
     3361 gaacgtaaaa ggtaactttt tctgttatct gaacgatcat aacgagaaca ccattgttga
     3421 cccttcggtg atctatttcg atttcgctaa cccgctgcag gctcaggagg tctgaatgtc
     3481 tgaggcaaac attattcaca gcagatatgg actgcgctgt gaaaaactcg acaagcccct
     3541 gaatcttggc tggggactgg acaatagcgc ggtgttgcac tgtcccgggg agctgccgac
     3601 agggtggctg tgcgatgcgc tggatcagat atttatcgcc gcaccacaac tgtcagcagt
     3661 ggctctcccc tgggccgaat ggtgtgagga gccacaggcg ctgacgcttt ttgggcaggt
     3721 aaaaagcgac attatccatc gtaccgcctt ctggcagtta ccgttatggt tgagttcacc
     3781 ggcaaaccgg gcctccggtg aaatggtttt tgatgcagag cgtgagattt atttcccgca
     3841 gcgcccaccc cgtccgcagg gtgaggttta ccgccgttac gatccacgta ttcgcaggat
     3901 gctgagtttc cgcattgccg atcccgtttc tgatgcagaa cgtttcaccc gctggatgaa
     3961 tgatccgcgc gttgagtatt tctgggagca aagtggctca ctggaggtac agaccgccta
     4021 tctggagcgc cagctcaccg gtaaacatgc atttccgctg attgggtgct tcgacgatcg
     4081 gccattcagc tattttgaaa tctactgggc ggcggaagac cgcattggtc gtcactattc
     4141 gtggcagccc tttgaccgtg gcctgcatct gctggttggt gaacagcaat ggcgcggggc
     4201 ccactatgtg caaagctggc tgcgcgggct gacacattac ctgctgctgg atgagccccg
     4261 tacgcagcgc acagtactgg agccacgcac cgataaccag cgcctgttcc gccatcttga
     4321 gcctgcggga taccggacaa ttaaagagtt cgacttccca cataagcgct cgcgcatggt
     4381 gatggcggat cgtcatcact tcttcacgga ggtcggtctg taatgaatca caaggactgg
     4441 gattttgtta accgtcggct ggtggcaaaa atgttgtctg agatggagta tgagcaggtt
     4501 ttccacgcgg aatctcaggg cgatgaccac tactgcatta acctgccggg agcacaatgg
     4561 cgcttcatcg ctgaacgtgg tatctggggc tggctgtgga ttgatgccca aacgttgcgc
     4621 tgcacggacg aaccagtact ggcgcagacg ctgctgatgc agctaaagcc ggtgctgtca
     4681 atgagtgatg ccactgttgc agagcatatg caggatttgt atgccacgct actgggcgac
     4741 ctgcaactgc tgaaggcccg tcgcggactg agtgccagtg acctgattaa tcttgatgcc
     4801 gatcgtctgc aatgcctgct gagcggtcat cctaaattcg tttttaataa aggtcgccgt
     4861 ggctggggca aagaggcgct ggaacgatat gcgccagagt atgccaacac cttcagactg
     4921 cactggctgg cggtaaaacg tgaacatatg atctggcgct gcgacaacga tctggatatt
     4981 cagcagttgt tgactgccgc aatggatccg caggagttta cacgcttcag tcaggtctgg
     5041 caggaaaacg ggctggatca caactggctg ccgctgccgg tacatccgtg gcagtggcag
     5101 caaaaaatcg ccaccgactt catcgctgat tttgccgaag gcaggatggt gtctctcggc
     5161 gagtttggcg accagtggct cgcccagcag tcgctgcgta ccctgaccaa cgccagccgc
     5221 cggggagggc tggatatcaa gctgccgctg accatctaca acacctcatg ctaccggggg
     5281 atccctggca gatatatcgc tgccggacca ctggcttcac gctggctgca acaggttttt
     5341 gcgaccgacg ccaccctggt gcaaagcggc gcagtgatcc ttggtgaacc ggctgcaggc
     5401 tatgtgtccc atgaaggcta tgccgcgctt gcccgggctc cctatcgcta ccaggaaatg
     5461 cttggtgtta tctggcggga gaatccgtgc cgctggctga aaccggatga aagtccggtt
     5521 ctgatggcaa cactgatgga gtgcgacgaa aacaatcagc cgctggcagg cgcatatatc
     5581 gaccgctccg gactggacgc tgaaacctgg cttacgcaat tgttccgggt ggttgtggtt
     5641 ccgctgtatc acctgctttg ccgctacggt gtcgcgctta ttgcacacgg acaaaatata
     5701 acgctcgcca tgaaagaggg ggtaccacag cgtgttctgc tgaaagactt ccagggcgat
     5761 atgcggttgg tgaaagaagc gtttcccgaa atggactctt tgcctcagga ggtgcgtgat
     5821 gtcacatccc gtctgagtgc ggactactta atccatgatt tgcagacggg tcacttcgtg
     5881 acagtactgc gttttatttc gccactgatg gttcgtcttg gcgtacctga aaggcgattt
     5941 tatcaactgc tggcagcagt gttgagtgat tacatgaaca aacatccaca aatgtcagag
     6001 cgttttgcgc ttttctcact cttcaggcca caaatcattc gcgtggtgct gaacccggta
     6061 aaactgacct ggccggacca ggatggcggc agtcgcatgc tgccgaatta ccttgagaat
     6121 ctgcaaaatc cgctgtggct ggtaactcag gaatatgaat catgaaaaaa agtgtcgatt
     6181 ttattggtgt cgggacaggg ccatttaatc tcagtattgc tgcattgtca catcagatcg
     6241 aagaactgaa ctgtctcttc tttgacgaac atcctcattt ttcctggcat ccgggtatgc
     6301 tggtaccgga ttgtcatatg cagactgtct ttctgaaaga tctggtcagt gcagttgcac
     6361 ctacaaatcc ctacagcttt gttaactatc tggtgaagca caaaaagttc tatcgcttcc
     6421 ttacaagcag actacgtaca gtatcccgtg aagagttttc tgactatctc cgctgggctg
     6481 ctgaagatat gaataacctg tatttcagtc ataccgttga aaatattgat ttcgacaaaa
     6541 aaagtcgatt gtttctggtt cagaccagtc ggggagaata ttttgcccgc aatatctgcc
     6601 ttggtacagg aaaacaacct tatttaccac cctgtgtgaa gcatgtgaca caatcctgtt
     6661 tccatgccag tgaaatgaat cttcgtcggc cggacctgag tggaaaacgg ataactgtgg
     6721 ttggtggagg acagagtggt gcagacctgt tccttaatgc attacgcggg gaatggggag
     6781 aagcggcgga aataaactgg gtctcacggc gtaataattt taacgcactg gatgaggctg
     6841 cttttgctga tgagtatttt acacctgaat atatttcagg cttctccgga ctgaaggaag
     6901 atattcgcca tcagttactg gatgagcaga aaatgacatc ggatggcatc actgccgatt
     6961 ctttactgac catttatcgt gagttgtacc accgttttga agttctgaga aaaccaagaa
     7021 atatccgtct gctacccagc cgctcggtaa caactctgga aagtagtggt ccgggctgga
     7081 agttattgat ggaacatcat ctggatcggg gcagagagag cctggaaagt gatgtggtga
     7141 ttttcgccac aggttaccgt tctgcattgc cacaaatact tccctcactg atgcccttga
     7201 tcaccatgca cgataagaac acctttaaag tgcgtgatga cttcactctg gaatggagtg
     7261 gcccgaaaga aaataacatc tttgcggtca acgccagcat gcaaactcat ggcatcgccg
     7321 aaccccagct cagcctgatg gcctggagat ctgcacgtat tcttaatcgc gtactggggc
     7381 gtgatttatt cgatctcagt atgccgcccg cactgattca gtggcgcagc ggcagccgga
     7441 aaaaaccgca gccggaggct gcttctttaa ctcactatac aacaaatatt caggaataac
     7501 gatgatgcgc aaaaagtata tgccccgggc tcttggtccg ctgcttcttg tcgtgttgtc
     7561 accagctgtc gcccagcaaa acgatgataa tgagatcata gtgtctgcca gccgcagcaa
     7621 tcgaactgta gcggagatgg cgcaaaccac ctgggttatc gaaaatgccg aactggagca
     7681 gcagattcag ggcggtaaag agctgaaaga cgcactggct cagttaatcc ccggccttga
     7741 tgtcagcagc cagagccgaa ccaactacgg tatgaacatg cgtggccgcc cgctggttgt
     7801 cctgattgac ggtgtgcgcc tcaactcttc acgttccgac agccgacaac tggactctgt
     7861 cgatcctttt aatatcgacc atattgaagt gatctccggc gcgacggccc tgtacggtgg
     7921 cgggagtacc ggagggttga taaacatcgt gaccaaaaaa ggccagccgg aaaccatgat
     7981 ggagtttgag gctggcacaa aaagtggctt taacagcagt aaagatcacg atgagcgcat
     8041 tgccggtgct gtctccggcg gaaatgacca tatctccgga cgtctttccg tggcatatca
     8101 gaaatttggc ggctggtttg acggtaacgg cgatgccacc ctgcttgata acacccagac
     8161 cggcctgcag cactccaatc ggctggacat catgggaacc ggtacgctga acatcgatga
     8221 atcccggcag cttcaactga taacgcagta ctataaaagt cagggggacg acaattacgg
     8281 gcttaatctc gggaaaggct tttccgccat cagcgggagc agcacaccat acgtcagtaa
     8341 ggggctgaat tctgaccgca ttcccggcac tgagcggcat ttgatcagcc tgcagtactc
     8401 tgacagtgat ttcctgagac aggaactggt cggtcaggtt tactaccgcg atgagtcgtt
     8461 gcggttctac ccgttcccga cggtaaatgc gaataaacag gcgacggctt tctcctcgtc
     8521 acagcaggat accgaccagt acggcatgaa actgactctg aacagccaac tgatggacgg
     8581 ctggcaaatc acctgggggc tggatgctga gcatgagcgc tttacctcca accagatgtt
     8641 cttcgatctg gctcaggcaa gtgcttccgg agggctgaac aaccataaga tttacaccac
     8701 cgggcgctat ccgtcatatg acatcaccaa tctggcggcc ttcctgcaat ccagctatga
     8761 cattaatgat atttttaccg tcagcggtgg cgtacgctat cagtatactg agaacagggt
     8821 agatgatttt atcgactaca tgcagcaaca gaagattgct gccgggaagg cgatatctgc
     8881 cgacgccatt cctggtggtt cggtagatta cgataacttt ctgttcaatg ctggtctgct
     8941 gatgcacatc accgaacgtc agcaggcatg gttcaatttt tcccaggggg tggcattgcc
     9001 ggatccgggg aaatattatg gtcgcggcat ctatggtgca gcagtgaacg gccatcttcc
     9061 cctgacaaag agcgtgaacg tcagcgacag taagctggaa ggcgtgaaag tcgattctta
     9121 tgaactgggc tggcgcttta ccggtgacaa cctgcggact caaatcgcag catattactc
     9181 gctttccaat aagagcgtgg aaaggaataa agatctgacc atcagtgtga aggacgacag
     9241 gcgccgtatt tacggcgtgg aaggtgcggt ggactacctg atcccggata ctgactggag
     9301 taccggtgtg aacttcaatg tgctgaaaac cgagtcgaaa gtgaacggtc aatggcaaaa
     9361 atatgacgtg aaggaatcaa gtccatcgaa agcgacagct tacattagct gggcgccgga
     9421 accgtggagt ctgcgtgtgc agagcaccac ttctttcgac gtaagcgatg cagagggtaa
     9481 cgatattaat ggttacacta ccgtcgattt tatcagtagt tggctgcttc cggtgggaac
     9541 actcagcttc agcgttgaga acctcttcga ccgtgactat accactgtct ggggacagcg
     9601 tgcacctctg tactacagcc cgggttacgg ccctgcttca ctgtacgact acaaaggccg
     9661 gggccgaacc tttggtctga actactcagt gctgttctga cggggattct tttgtaacaa
     9721 gggtatgctg taaacaacat ggtccttggt ggccatgttg tttacagcca gtataatata
     9781 cagccttaac aagatattcc gtctccggaa tggactgttc accactgaat gaaccgcccc
     9841 gggtttcctg gagagtgttt tatctgtgaa ctcaggctgc cagatcattg tttccgatgg
     9901 aagcataata agctttttct gcttctgccg gaggagtatg gcccagcctt cccagcaatc
     9961 gtcgattgtt ataccagtcc acccacgtta gtgtggccag ttccacttct gtccggtttt
    10021 tccagctctt acggtgtatt acctccgctt tgtaaagacc attgatgctc tccgccatcg
    10081 cgttgtcata cgagtcgcca gtact
//
LOCUS       DAZGVQ010000181         9844 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000181 DAZGVQ010000000
VERSION     DAZGVQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.182"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..242)
                     /locus_tag="ACHLAG_004432"
     CDS             complement(<1..242)
                     /locus_tag="ACHLAG_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0657552.1"
                     /translation="MAGNFADLTAVLTLDSTRFSEEAARVKKELGETSDLADLMAGRV
                     SQSFKKQAAAVEQGLSRQALAAQKAGISVGQYKAAMR"
     gene            complement(232..636)
                     /locus_tag="ACHLAG_004433"
                     /pseudo
     CDS             complement(232..636)
                     /locus_tag="ACHLAG_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241241.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(663..1094)
                     /locus_tag="ACHLAG_004434"
     CDS             complement(663..1094)
                     /locus_tag="ACHLAG_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP0657553.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEEAEVSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTASPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNALTDDDFSAGKSSTAS"
     gene            complement(1110..1859)
                     /locus_tag="ACHLAG_004435"
     CDS             complement(1110..1859)
                     /locus_tag="ACHLAG_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0657554.1"
                     /translation="MATPNPLEPVKGSGTTLWVYTGTGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWVSTGQGQKSAGDTSFTLAWKPGEKGQRDLIAWFDS
                     SETRAYKIRFPNGTVDVFRGWVSAIGKAVTAKEVITRTVKITNIGRPSLAEDQGDITP
                     VTGITVTPPTGNVAKGQNITLTVAVQPEGATDKTFRATSANQNFATITVKGNTITVKG
                     VAAGKAQIPVVTGNGEFAAVAEINVTDGAAG"
     gene            complement(1867..2265)
                     /gene="gpU"
                     /locus_tag="ACHLAG_004436"
     CDS             complement(1867..2265)
                     /gene="gpU"
                     /locus_tag="ACHLAG_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFP0657555.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSVLDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKITYEM"
     gene            complement(2278..2901)
                     /locus_tag="ACHLAG_004437"
     CDS             complement(2278..2901)
                     /locus_tag="ACHLAG_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0657556.1"
                     /translation="MAIKGLAQAMKNLDAIDSRAVPRAAATTLNRVAESIIAKTASSV
                     ARELAVPRRLIRERIRLQRASADRVYAKVIINTGNLPAIKLGTASVRLSRRKRRKKGE
                     RSVTKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKIPTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            complement(2904..3185)
                     /locus_tag="ACHLAG_004438"
     CDS             complement(2904..3185)
                     /locus_tag="ACHLAG_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFP0657557.1"
                     /translation="MSDLFTRMCCRMDGATVRVMGKQAEINGVVYDVMPEEESADMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(3178..3504)
                     /locus_tag="ACHLAG_004439"
     CDS             complement(3178..3504)
                     /locus_tag="ACHLAG_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFP0657558.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     DAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            complement(3592..5571)
                     /locus_tag="ACHLAG_004440"
     CDS             complement(3592..5571)
                     /locus_tag="ACHLAG_004440"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFP0657559.1"
                     /translation="MKNKSWFRMQAGGQGEADIYIYDEIGFWGVTAKQFVSDMNALGD
                     ITHINLHINSPGGDVFEGIAIFNALKNHGAAITVYVDGVAASMASLIAMAGDTVIMPE
                     NAFMMIHKPWGISGGDAEKMRTYAERLDKLESVMVPVYAQKTGKTTDEIAAMLADETW
                     MSGAECLAHGFADQVTPAVKTMACIQSKRTEEFKKMPESIRNMITQPYNSAPRDTTVT
                     IPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMFGGRYQALQAQCVADP
                     DCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIRQAMLARAGFENVEKD
                     NAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDFGNILLDVSNKGLIQG
                     WEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEYKYITTSDRKETIALA
                     TYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKVLTDNPKLSDGKALFH
                     ADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVPVALETVANQTIKSAS
                     VKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTDTIEVAWLDGVDTPYI
                     DQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            complement(5561..7063)
                     /locus_tag="ACHLAG_004441"
     CDS             complement(5561..7063)
                     /locus_tag="ACHLAG_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0657560.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFIRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKDTNREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTEQQRQQATDSQHEE"
     gene            complement(7063..7275)
                     /locus_tag="ACHLAG_004442"
     CDS             complement(7063..7275)
                     /locus_tag="ACHLAG_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657561.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            complement(7272..9395)
                     /locus_tag="ACHLAG_004443"
     CDS             complement(7272..9395)
                     /locus_tag="ACHLAG_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP0657562.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPSRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNKGIWLREGERAVADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQECSGETVSPELTAGAEK
                     RRRRKKKLRTERAEDNPWITSGGWL"
     gene            complement(9392..>9844)
                     /locus_tag="ACHLAG_004444"
     CDS             complement(9392..>9844)
                     /locus_tag="ACHLAG_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFP0657563.1"
                     /translation="KLNINQLAALSGLHRQTVAARMADVPLAPGSNEKKKLYLLTDLI
                     ISLLEKPPTSEDEEMNPHDRKAWYQSERERLKFQHETVQLVPVSDVRRSFSVVVKAIV
                     QVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
BASE COUNT         2118 a         3093 c         2351 g         2282 t
ORIGIN      
        1 cgcatggccg ctttatactg cccgacggaa atcccggctt tttgtgcagc cagcgcctga
       61 cggctcagcc cctgctcaac agcagcggcc tgtttcttaa aagactggct gacacgcccg
      121 gccatcaaat ccgcaaggtc actggtttca cccagttctt tctttacccg tgcagcctct
      181 tcagaaaaac gggttgaatc cagtgtaaga acagctgtca gatcggcaaa attacccgcc
      241 atagcgtaca ccccctggaa ttccctcaga caccatcatc agcatggctt catcctctgt
      301 actgctccgc atgtcatcac tgaccataac gatttacttc ccgtcagccc caaagcggac
      361 accaccagaa agacctgccg ctttccgcat cagcatatcg tcctcatccg gcatctccgt
      421 ctgctcatca tcacgtcggg atgccagcag actgaaatca gagggatgca tatccggatc
      481 gcaaaaaaac aggctgagta cagcgtacgt cagcccggaa aaatgcatat ccagctgggt
      541 atcctgaaaa taatgcgtgc ggtaaaaacg gtgccagtcg gcatattcgg tggatgtcat
      601 cccggcaagc atggcgcgcc agtcgggtct ccccatctca cgcgccagtc tgagggcaaa
      661 gttcagctcg ccgtcgaaga ctttcccgca gaaaaatcat catcagtcag cgcgttattt
      721 ttcgccactt cagtaatatc agtatccgga cgaacagctt cgatcatccc ggacaggcac
      781 aacacaacgt cttccgcccg ggcaatggca tcggcaggcc aggtggtgag cacttcctgc
      841 tctatcttca tcacggcctc attcattgac ggtgacgccg ttttctgtgg atggttatgc
      901 cacagggaca tcgccaccag aaacgccccg gttctgacaa ggtcctccac gctcacctgc
      961 aggttaccgc tgacttcagc ctcttctgcc cgccgtttca ggagggcaag atgctcaata
     1021 cgctgcagcg cagacagctc agaaagcgtg acggacacac cgttatattc aaattgttct
     1081 gttttcagaa acatgcttta tctcccccct cagcccgcag cgccatccgt gacgttgatc
     1141 tccgccaccg ccgcaaactc accattgccg gtgacaacag ggatctgcgc tttacctgcc
     1201 gcaacacctt tcacggtgat cgtgttcccc ttcacggtaa tggtggcgaa attctgattc
     1261 gctgaggttg cgcggaacgt tttatccgtt gcgccttccg gctgaacagc cacggtcagg
     1321 gtgatattct gaccttttgc cacattgccc gttggtggcg tcacggtaat accggtgacc
     1381 ggtgtgatgt ccccctgatc ttccgccagc gacggacgac cgatattggt gatttttacc
     1441 gtacgggtga tcacctcttt ggcggtcacc gctttaccaa tggcgctcac ccagccacgg
     1501 aacacatcca ccgtaccgtt cgggaagcgg attttgtagg cccgggtctc actgctgtca
     1561 aaccaggcaa tcaaatcgcg ctgccctttc tcgcccggct tccaggccag cgtaaaactg
     1621 gtgtcaccgg cagatttctg cccctgaccg gtggataccc agtcagcatc ctcatcatcc
     1681 agatagttat catcgtagga ttccgccgtc atctcgccgg gggtcagatc cttcaccttc
     1741 gccagtcgct gccagtcatc gtctgacaac gggtttgcat aagcatcacc agtgccggtg
     1801 tacacccaca gtgtggtacc ggaacctttc accggctcaa gaggatttgg tgttgccata
     1861 tcgtcctcac atctcgtagg taattttcca caggagatct gccgatcccc acatcataaa
     1921 ctcatcatcc cggcggtagt catacccctg aagattcatc ttcagcagta acgcactgag
     1981 gccgggaacc gcctccagcg caggaagaat tttctcttcc atccacatat ccagtaccga
     2041 gtccggttct tttgccctga gaaaaacttc aatatgcagt gtcgcctccc aggttccctc
     2101 atcaacgaac tcgtcagcag cagacgcatc tgtcaggtaa acagcaacag caggcagttc
     2161 ctgttcatca ataaaaaccg ggcgaccgtc aaaccagctc accctctcag aaatattttc
     2221 tttcagggca gacagaactg ccgcccgtat ttcacggtgt ttcatacacc ctcccggtca
     2281 ttttcttttc agcaccagac gtaactgatg cgtcatggct ttcatcatct gcgccggtaa
     2341 tttttcccgg tacatccggt cccgttcacg ttcaaaggtt tctgccagcg gtccggcagt
     2401 cggaatcttc accacttcga tcggcagacg gtggcgtttc ggcctccctt tgctgtcagc
     2461 gccggtggac gatgatgccc acggcatacg ctgcatcaca tgccagcgtc cgttagccag
     2521 ccgggtgata aaggcatccg ggatccgtct tttccccaca atcagcacac tgccaccgcc
     2581 tttcgtgacc gaacgctcgc ctttctttcg tcgctttctg cgggaaagcc gcacgctggc
     2641 cgtccccagt tttatggcgg gcagattacc ggtgttgatg atgaccttcg cataaaccct
     2701 gtctgcgctg gcccgttgca ggcggatacg ctcacggatg agacggcgcg gaaccgccaa
     2761 ctccctggca accgaagagg ccgttttcgc gatgatggac tccgccacac ggttaagtgt
     2821 cgtggcggca gcccggggaa cggcacggct gtcaattgca tccagatttt tcatggcctg
     2881 cgccagacct tttattgcca tactcattcc tgttcaacaa aaatccgggg tttaccgttg
     2941 tacgtgtcat aacgggtcac cgtcagtgtg cgccccgcaa acacaacaac atcatgacgg
     3001 gccggacggt accgggctga aaacaccacc agtgacaact ggctgcccga aagcgccccc
     3061 atatccgcgg actcttcctc cggcatcacg tcatacacta cgccgttaat ctccgcctgt
     3121 ttgcccatca cacgaacggt cgccccgtcc atccggcaac acattcgcgt aaacagatca
     3181 gacattgatt tttaccgcca cagtggcgct gtttgcaggg gcattttccc aggctacccc
     3241 cgcagccacc gcaccgtctg cagccagctg cacaaccccg tccttcagat aaaccaccgc
     3301 gccggactga atatcgtcag cagactgttt gggcagcagg aacacgcctt cggcaaaacc
     3361 gtcaccggca tcaccggcag gaatatcggt aatggccact gccaccatac tgccgaccac
     3421 caccgcagca ccgctcagga tggtctgatc tccggcattc accagttcaa tggtggtgcc
     3481 gtcctgtaca aaatttttcg ccataatgct gtttctccgg acagcccctg tggggctgtt
     3541 tttcaggcat aaaaaaagcc ctttcgggca gtgattgtga taacgcggtt atcaggccac
     3601 cgacgaacgc accagaccgc gccagtcaag tggtgccaca ccggcatcaa tacgaatttt
     3661 tgtggcaatg ccgtcagtgg tgaaaccttc ctgctgatca atgtatggag tgtccacacc
     3721 atccagccag gccacttcaa tggtgtcagt gccctgtgcc gccgccagat accaggtttt
     3781 cggatctgcc gcatcaagac gcgcttctgc aatcacctca gcaaagttct ggatggggtt
     3841 aatgacaccg gcgtttgcat ccgccccttt cacactggcc gatttgatgg tctggttcgc
     3901 caccgtctcc agtgccaccg gtaccagcat aaaggccgga cggatattca gggcgcgatc
     3961 gccttctttc tgcaggcgca tcatctgacg ggccgcatcc agtccggaaa cggaaatccc
     4021 accggtggca atatttttgt gatcggcatg gaacagcgcc ttaccgtctg acagtttcgg
     4081 gttatccgtc agcaccttgt aaaccaggtc accaatcgtt gccttcgccg cacgccccat
     4141 cttcatcggc acgtccacca gcatattcag atcatcattg ataatggcct ggcgggtgat
     4201 ggagaaaatc tccccgtaag tggccagtgc aatggtctcc ttgcgatctg aggtggtgat
     4261 gtatttatac tccgccccct cacgaacctg gcgcagagaa ccaaaaccgc ccatccccac
     4321 gcgatacgct gttttgaagt ctgacaggcg tcccttacgg gtccacttct ggaaggtttc
     4381 ttctgattcc tcccagccct ggatcagccc cttgttcgac acatccagca gaatattgcc
     4441 aaaatcagag gtgctgtgcg tcagcgccag cccgaccatc tgcatggggt tataactggc
     4501 caccccaata ccgcgctccg tcagtgacat gcgagcccat tcacgcaggg tcatcccgtt
     4561 ataggcgtta tccttctcga cattttcaaa tccggcacgg gccagcatcg cctggcggat
     4621 cccgtccccc acaaaattgc cgtttccggc ataaatatgg gccggtgtgt ttttgttggt
     4681 cggcgaggac tccttgccca tttcattcag cagacgttca cgggccattt ccagcgaaca
     4741 gtcaggatca gccacacact gcgcctgaag cgcctgatag cgaccgccaa acatggcaaa
     4801 cagatcgtta atgcctgaca tgcgggcttt ctgctcagcc ataacgcggg cgcgaatggt
     4861 cgcctcatca gacactgccg gtaccggtga tggttctgtt accgccggtg cagggattgt
     4921 cactgtggta tcacgcgggg cactgttgta tggctgcgtg atcatatttc ggatggattc
     4981 cggcatcttt ttaaattcct ctgtacgttt tgactgaata catgccattg tcttaacggc
     5041 tggcgtcacc tggtctgcaa atccgtgtgc cagacactcg gcaccggaca tccaggtctc
     5101 atccgccagc atggcggcaa tttcatcggt agtttttccg gttttctgcg catataccgg
     5161 caccataacc gactcaagtt tgtccagacg ttcggcataa gtgcgcattt tctccgcatc
     5221 accaccgctg atcccccagg gtttatggat catcatgaag gcattttccg gcataatgac
     5281 cgtgtcaccg gccatcgcaa tcagggatgc catcgaggcg gcaacgccat ccacatacac
     5341 ggtaatggcc gcaccgtgat ttttcagggc attaaaaatg gcgatgcctt caaagacatc
     5401 gccacccggt gagttgatgt ggagattaat gtgggtgata tcacccaggg cattcatatc
     5461 gctgacaaac tgcttcgcgg taactcccca gaaaccaatc tcgtcataaa tataaatatc
     5521 cgcctcaccc tgaccacccg cctgcatcct gaaccaggat ttattcttca tgctggctgt
     5581 cggtggcctg ctgacgctgt tgttcagttc cggcactgtt gcctcctttg tcgttgacgg
     5641 ggtcagtatc aaagaccagc cccagtctgc tgttttcatc aatttcagcc ttgcggcgac
     5701 gtttgacctc atccggattg cgcccgcctg cacgcaccca gtcagattct gtcgctgcac
     5761 caccccggat ctgaattctc caggcttcag cttccttaac cgggtcgatc cacggcatca
     5821 ccggaccgga atacgtcgcg ttatatagcg ttttcatctc cacatccgcc ggaattttca
     5881 gcagacctgc cgcaaccacc atattcagcc atgtccggta caccgggcgg gttaccgcgc
     5941 caataaaaca gtcctgcagg atcaggtaac catccgtgga ctcgaccagc tcctgccgct
     6001 gggcactgta ggtgccgtta tagttacgcg ccgcactgga aaaactcaga cgactgccag
     6061 ctgccactgc acgcaactgg ccgttgcgga aagtttcaag gttgggattg ggacggtcag
     6121 atttgaccat gccgatatcc tcgcccttgc gcaaatcgtc ataaataata cccggggtga
     6181 tatggacttc ccggttggta tctttgatcc ccggatcttc atagtcctgt ccgtcccctt
     6241 tacggatata cagtcccagc gccgcggcaa tacgcgcagc cgtcagttcc gcatcctcat
     6301 attccttaag ggcactgatc cgcatcagca cccccgataa catggatgag cctcgcgtct
     6361 gatgcagacg gcgtatgaac ttcaggtgga tcatttttcc ggcagcgatt tctttcgtat
     6421 cactctgccg accgctgacc ggataatttt tataaaccag atattttttc ggtcttcccc
     6481 actcatcaag aaaaaccccc tgattcagcc cggcggattc atcagtgcgc atgggaacaa
     6541 aatccggctc catcgcctca agccagaatg gcactcccgc cgtccgttcc agaccgtttc
     6601 ccgcgccact gaccatctgc gcaaacactt caccatcccg cagccaggtc cgcagcagta
     6661 aacgttcaag cacgggacgg gtatactgcc ctgtcacatc cggactcacg gaccattcag
     6721 cccacaaccg gcggatatcc gcagccagct ctgccgccat ttccccgttt tttcgtaatg
     6781 gctgaggctc cacaataatt cccctggcac caatcacccg ctcttccagc ttgtcaaaca
     6841 caccaatcac caggtcatga ttgatatcca gaaaacgggc ctgctcccgc agggaaaccg
     6901 caccgtattt actgagctga tcagcagagc gattttcccg ccgggcttta tgtgtccggg
     6961 tcggtttcac cgcctcatag gccatgatta acgcccttga acgcagtctg gctgctttcc
     7021 acccggggga aaacacgcct atcacatcat caataattgc cattaaaacc tcgccagttt
     7081 aaatcctggt tttccccgcc tgcggctcac catcgcggca agcctgcgtt cccactcctg
     7141 acgtccggcg cggatctgag aaaggctttc cagcgtcagt tgctgtccgt tgaaggtgac
     7201 agacttcccc tcaagtacgg ccatttccgc ttcacggtac cgctgtatca tttctctggc
     7261 ttcttctgtg ctcacaacca gcctcctgat gttatccatg gattatcttc cgcacgctcc
     7321 gtccgcagtt ttttcttccg gcgacggcgt ttttctgccc cggccgtcag ttccggggat
     7381 accgtttcac cagaacactc ctgcgggaag acgagccacg tttcccgctg tgcccagtcc
     7441 ggtgcggagg gccagcggat cttttcgtaa ccatgcagaa cggcaagcgc atccgcataa
     7501 accagcaggt caaacgcctc gttagctccc ctgcccggtt ttcgccattt tccgtcactg
     7561 ccgcgctctt cataggtcag ctcatcgtaa aaccatcgcc ccagccagtc gggaaagtgg
     7621 atatagttcg gccctggtgt gtcacgccac agggcattat ttacacggtc cttaaacgca
     7681 tccgtctgaa ccagccacag cgcgacatcg ccactggctc tggcacggcg ggcacttctg
     7741 ccggtattat ccgggaaggt acggttaatc agcctgtcac ggcgaagtcc atcccccttg
     7801 aacagaaaca ccctgttgcc cagtccgtca ctccggcaac gacgccagaa acgataggcg
     7861 ttatctgtca ccccggcttc ccctcccgta tccaccgcca tggccatcag acgcatgcgc
     7921 acatccggat cagaagccag cggccatgtt ttatggaaca catccgtcag caacaaatcc
     7981 cagtcctccg gatatgccgc cggatcaacc ggcagacttt caccgttggg actgcagcgc
     8041 agtgaatgcc ggatgttata gcgatcaaca atccagcgtt ccccctgctc tccgtatccg
     8101 gtgatctgca caacaaaacg gcgattttta ccgccctgta cgtcaaccgt tgcctcaata
     8161 aaacgcacac catccggcac agatcgccgg ggaaacggct cggcacgctg ttcaagcagt
     8221 tcacttttac gctgttccgt ggctgaacgg ggcagatagg gtcgtccgat atcggtgttc
     8281 accaccgctt tcagggtctc ttcactgccg gttcgctcat actcttcttc tgccgccagc
     8341 agtttaaaaa tcagttgttc ccaggtctga aacgccgcgg ccggcccctc catccagaat
     8401 gacgcaatcc gggaatttcg tggcgttccg gtgatactgc cgtccgccac cgcccgttca
     8461 ccttcacgaa gccagatccc tttgttattc agttcgcgtt tctgctcagg ggcaatcagc
     8521 ccgcgacaat gcggacacat cagacgggca gcctgaccgg cagccacaaa atccgggtta
     8581 ttccggtatc cggtcatgtt atccatcacc ggctgaaaat attccccgca gtgcggacac
     8641 ggccagtacc accggcggcg gtctccccgg ttatacagtg acaggatccc agttgttggc
     8701 ggtgcctcat gtgcgccgcc acagcgccat ttggtgtcag tgatatcccg ccccggcgaa
     8761 ctctcgacca gggtcatccc cgaggacata aaggtggtgg tacgctttga tgccagggtg
     8821 aaggcatccc cttccccgtc cacgttttca gggaaacggt cataatccgt cagcgccaca
     8881 cgacggtaat ccgaagaaga aaatacagtg atcgacggcc agccaatctt caggaaggag
     8941 ccgtcaagaa acattttatc gtggacgttg ttgtcattac gggaaggact gagacgctta
     9001 ctgacctccg ggctgtgacg aaacgttctg gaaagacgcg ttctggagtg ttcccgcgcc
     9061 ttcgtctcgg tcatttgcac caccagcata tccgccggat cacagatgat gccgtacaca
     9121 atccagccgt caatcagccc ttcggttttc ccggttcgcg caggtcccac aaacaccacc
     9181 gcgtcatatt cacgggctga taatgtatta atggggtcaa tcatataggg cgtcagcgat
     9241 gactcccacg gaccggaagt attggctccc cgtggtaccc gcatataacg cctgatggct
     9301 tccgctactg gtaaccggct gggcgggcga aacagcgagg ccacttcgcg ccagatatcg
     9361 gatgcgcggc tatggctctc gttcacctga ttcacatatc ggcctcatca caacagtcaa
     9421 tgactgcctt ttccagtgtg tcgcggatct catcaaccac aatctgtact tcattcagtt
     9481 gtgatgcggt ccaccctctg tccctttcca gccggtcagg ccaggtttcc agtacctgaa
     9541 ctatcgcttt caccacgaca gaaaaggacc gcctgacatc actgacaggc acaagctgaa
     9601 cagtttcatg ctgaaattta agacgctcgc gctcggactg ataccatgcc ttccgatcat
     9661 gtgggttcat ctcttcatct tcggaagtcg gtggtttttc cagcaacgaa ataatcaaat
     9721 ccgtcaggag atacagtttt ttcttttcat tactgcctgg tgcaagagga acatccgcca
     9781 ttctggcggc aacagtctgc cggtgcagac ctgaaagggc tgccagttga ttaatattta
     9841 actt
//
LOCUS       DAZGVQ010000182         9805 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000182 DAZGVQ010000000
VERSION     DAZGVQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.183"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(77..268)
                     /gene="ydfD"
                     /locus_tag="ACHLAG_004445"
     CDS             complement(77..268)
                     /gene="ydfD"
                     /locus_tag="ACHLAG_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HFP0657564.1"
                     /translation="MNSAFALVLTVFLVSGVPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNIEIPAGL"
     gene            complement(265..453)
                     /gene="dicB"
                     /locus_tag="ACHLAG_004446"
                     /gene_synonym="ftsT"
     CDS             complement(265..453)
                     /gene="dicB"
                     /locus_tag="ACHLAG_004446"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP0657565.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            958..1278
                     /locus_tag="ACHLAG_004447"
     CDS             958..1278
                     /locus_tag="ACHLAG_004447"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657566.1"
                     /translation="MSQPPINPLKNMKIDYWYKALTVVGAALFVFNGTSFFDRYPVVP
                     LGFLSSGIFFIGLGEWINHPLKVRFIGPGVWTRGYNSSSCALGIIFDILGCFLIVTGV
                     VKFF"
     gene            complement(1414..1515)
                     /gene="ydfB"
                     /locus_tag="ACHLAG_004448"
                     /pseudo
     CDS             complement(1414..1515)
                     /gene="ydfB"
                     /locus_tag="ACHLAG_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
     gene            complement(1517..1672)
                     /gene="ydfA"
                     /locus_tag="ACHLAG_004449"
     CDS             complement(1517..1672)
                     /gene="ydfA"
                     /locus_tag="ACHLAG_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP0657567.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(1865..2272)
                     /locus_tag="ACHLAG_004450"
     CDS             complement(1865..2272)
                     /locus_tag="ACHLAG_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0657568.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNGLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPAPPVSLDSALDLSEDELEMLRLYRALPKS
                     EQQAQLSELRARVENFNRLFTELLEARKRNKHQ"
     gene            2350..2577
                     /locus_tag="ACHLAG_004451"
     CDS             2350..2577
                     /locus_tag="ACHLAG_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP0657569.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWQELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKTKRAGRLNNENHS"
     gene            2561..3082
                     /locus_tag="ACHLAG_004452"
     CDS             2561..3082
                     /locus_tag="ACHLAG_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP0657570.1"
                     /translation="MKITPEQAREAMDAWICRPGMTQEQATILITEAFWALKERPNID
                     VQRVTDEGGAVDQRALGVNRVKIFERWKAIDTRDKREKFTALVPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYEPSFC"
     gene            3063..4028
                     /locus_tag="ACHLAG_004453"
     CDS             3063..4028
                     /locus_tag="ACHLAG_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657571.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     GSELLDDGKLSESIGSKCAAPSGQNDTMEEVKMKRSIGSKRSNVIGSKWPDDLTENTT
                     EITTENKNTFRPEASQPDPQTTEQDFLTRNSDAVVFSAKKRQWGSREDLACAQWIWGR
                     IVGLYEQAASDDGEIMRPKEPNWTVWANDVRTMRMLDGRSHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            4069..4491
                     /locus_tag="ACHLAG_004454"
     CDS             4069..4491
                     /locus_tag="ACHLAG_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP0657572.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLAREQVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKSEVMQRVLTFYQGNYREVML"
     gene            4488..4664
                     /locus_tag="ACHLAG_004455"
                     /pseudo
     CDS             4488..4664
                     /locus_tag="ACHLAG_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004203.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
     gene            4775..5440
                     /locus_tag="ACHLAG_004456"
     CDS             4775..5440
                     /locus_tag="ACHLAG_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFP0657573.1"
                     /translation="MTVADFKRPKLELPNGAKKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAKQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREQFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     GQE"
     gene            6053..6208
                     /gene="hokD"
                     /locus_tag="ACHLAG_004457"
     CDS             6053..6208
                     /gene="hokD"
                     /locus_tag="ACHLAG_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP0657574.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            6425..6676
                     /gene="rem"
                     /locus_tag="ACHLAG_004458"
     CDS             6425..6676
                     /gene="rem"
                     /locus_tag="ACHLAG_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Rem"
                     /protein_id="HFP0657575.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRILCLRKNGAALIN
                     FVDARVTDGFILREGEFVTSLQALKEIGIKAGFSAFAEE"
     gene            6743..7021
                     /locus_tag="ACHLAG_004459"
     CDS             6743..7021
                     /locus_tag="ACHLAG_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657576.1"
                     /translation="MARNVKYYNSDNSPVLVCTHERYSHAFKSEWFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRASRTFQQRIWR"
     gene            7023..8072
                     /gene="ydfU"
                     /locus_tag="ACHLAG_004460"
     CDS             7023..8072
                     /gene="ydfU"
                     /locus_tag="ACHLAG_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0657577.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESIQIFHNPRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKTLLPFFSNERVIRAAGGVGALSDWLLRHVTSCQWPNGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLDLLAQQNLTAWVIDVIRHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLPAEKICSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPYAHQQQKLPQEKTVVSITVDPESPESFMKLPKRRRWVKEKYTRWVKTQPC
                     ACCGMPADDPHHLIGHGQGGMGTKAHDLFVLPLCRKHHNELHRDTVAFEEKYGSQLEL
                     IFRFIDRALAIGVLA"
     gene            8086..8838
                     /gene="ydfT"
                     /locus_tag="ACHLAG_004461"
     CDS             8086..8838
                     /gene="ydfT"
                     /locus_tag="ACHLAG_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP0657578.1"
                     /translation="MNLEALPKYYSPKSPKLSDDSPATGTGCLTITDVMAAQGMVQSK
                     APLGLALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEEIRLQIIPYLVSFAFAD
                     YSRSAASKARCEHCSGTGFYNVLREVVKHYRRGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRFHGVPVYKICGRCNGNRFSRLPTTLARRHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEAQLRKVTR"
     gene            complement(9013..9215)
                     /gene="cspF"
                     /locus_tag="ACHLAG_004462"
                     /pseudo
     CDS             complement(9013..9215)
                     /gene="cspF"
                     /locus_tag="ACHLAG_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
     gene            9514..9729
                     /gene="cspB"
                     /locus_tag="ACHLAG_004463"
     CDS             9514..9729
                     /gene="cspB"
                     /locus_tag="ACHLAG_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HFP0657579.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSIESGAKGPAAVNVIITD"
BASE COUNT         2645 a         2074 c         2468 g         2618 t
ORIGIN      
        1 acagatttaa ggatgaacaa atccctgcca ttgctggcat ataagaatga aaccggatat
       61 ttattacgga actgttttaa agacctgccg ggatttcgat attatcctgg tgaataactt
      121 tatcgaccgg gtaacagtta ccgggaattt tctgttcggt tgctgcagtc atacactcct
      181 gcattgtcct gtgaacactg actgcaatat caactggcac tccggaaaca agaaaaactg
      241 tcagaacaag cgcaaatgct gaattcattg tgcacatcct tttggcatca gacgtaaacg
      301 agccagcatt gaaacaatgc atattttatt taatagctcc cgttcttgtt ttctcttgtt
      361 aatggcatct tcagtaaata cagggttact gatagtgaca ccaatttcaa aacaaccttc
      421 agacgtatta acgtttggta ataacgtttt cattatcgcg tcctcaacaa tgaattttgt
      481 gatgcagtgc ctggtgcctc caggtgacgt taaccagtta acaattaacg tcggatacag
      541 agaatccacc cataacactg tttttggttt taactgttcc gcgtgcgctt agccgcattc
      601 accgcatcac aaaattcact ttaaaaacgg cggcagagca gtcacggagt aaaactgata
      661 ccgccaaacg tcaccagaaa attgataaca gagggcgttg cagcggggtt gtcacttaag
      721 cgtatggtca acctgacaac tcggtgtcct caacggggaa ggaataaccc cgccatactt
      781 accgccgcgc cattttgcgg gttgccacaa ccggaagcgc acggtcgaat taaatttaac
      841 gacaccgtac agcgagacga acttcgccgt gcgctttcgt gttgtgtgcc tgcttttaac
      901 cacgtcaggc gaggtggtat ccttaaaatc accacagttt taaggattca ttaagcaatg
      961 tcgcaaccac caataaatcc gcttaagaac atgaaaattg attactggta taaagcgctt
     1021 acagttgttg gcgctgcgtt gtttgtcttt aatggaacgt ctttttttga cagatatccc
     1081 gttgttccat tgggtttttt gtcctccggc atctttttta ttggtttggg ggagtggatt
     1141 aatcaccctc tcaaagtgag atttattggt cctggagttt ggactcgtgg atataatagt
     1201 tcttcgtgcg cactcggtat catcttcgac atacttggtt gtttcctgat tgttacagga
     1261 gtcgtcaagt tcttctgatg taaaaccgca aatggggcac gtaacgggaa ttttgaaaag
     1321 cgtttctcag ggttccagaa caaaattttc tgcggtctga ttttgcttct catatttgtg
     1381 ctccgcgtca ttgtgagagc acattcttat tctgagtgcc tgtttaaact cactgaagct
     1441 gagagcttct tcgccttcgg caaggccttc gaagtattct tcgtaagcct tttccatgat
     1501 tgtgtcgaaa tccatatcac tcacctgagt ttctttccag ccagcgacgg gcaccatttt
     1561 cggttttaaa cgttttgctt ttggtatacg tcatcgcggt gaacgtaccg tcctggttgg
     1621 ggaatacgcc acataccaga gattcgttgt tgccaagatc gatagtatcc atgctgacct
     1681 catttcccct taacgccggg gtagcggaac tgtttgctga gaacaccgtg cggtgtcttg
     1741 atggaggtaa tttagttttc tcattgtttt tcgtcaaggt tttttgatga gaaaactcaa
     1801 gtattggcgc aagataaagc caatacattg aaatgtaagg ctttaaaatt ttgtgaaggg
     1861 atgattattg atgcttgttg cgtttgcgag cctctaatag ctcggtgaac aggcgattaa
     1921 aattctcaac gcgggcgcgg agttcgctga gttgtgcttg ttgctctgat ttaggaagtg
     1981 cgcgatacaa acgcagcatc tctaactcat cttccgataa gtctaaggca ctatcgagtg
     2041 aaacaggggg agctggtgtt ttgtcctcgt cgccaaacag tatccaggtt ggtgaacatt
     2101 gcaatacctc ggcgaggcga tgcaaatttt gcccgcgcgg ggctgtatgg tcactttccc
     2161 ataatgaaat tgatgagcca gatacgccag cagctttgct taaaccgttt tgacttaagc
     2221 ctacctgctt acgtctttct ctaatgcgtt gacctaaagt tttctcgttc atatttagat
     2281 atcttaataa cccttgactt gagattcctt gagtgattag tattgagaaa actcaatatt
     2341 ggaggtgcga tgtttaaatc agacgtaatt aatttttacg gtacgaaagc caaagtagcg
     2401 aaagctgctg gcgttgaccc atctgctgtt tctcaatggc aagagctggt tcctgaaggt
     2461 cgcgcgatgc gtctacagga ggcatctggc ggcgagcttc agtatgatcc caaggtttat
     2521 gacgaatatc gtaagacgaa gcgggcgggg cggttgaaca atgaaaatca ctcctgaaca
     2581 ggctcgtgag gctatggatg cctggatatg tcgaccagga atgacacagg agcaggcgac
     2641 gatattaatc actgaagcat tctgggcttt gaaagagcgc ccgaacatcg atgttcagcg
     2701 tgtcacagat gaaggtggcg cggttgatca gcgagcgctt ggcgttaatc gagtgaagat
     2761 attcgaacgc tggaaggcta tcgacaccag ggataagcgt gaaaagttca cggcgctagt
     2821 gcctgcaatt atggaggcta tccggattaa tgatttcagg ttgtatcgtg aaattagtga
     2881 cggaaaaagc atcacgtaca tgatcgccgg gttaaacaaa gaatatggcg atgtggtgga
     2941 gtccggactg ctttttgctg atcctgccgt agtggatcgt gaaactgacg aacttataga
     3001 aaaagcaatt gctttcaaac ttgcgtatcg acagcaatac caacaaaaag ctggatggaa
     3061 ttatgagcct tctttttgct gaacgcccac tggttataaa cacacagctt gcgatgaaga
     3121 ttggcttaaa tgaagccatt gtgttgcagc agttgcatta ctggttgaga gataccaatt
     3181 ccggtatgga atgtgatggt gttcgctgga tttacaacac aacggaacaa tggctggaac
     3241 agttcccatt ctggtcagag tcaacgttaa agcgcgcgtt tgcaagtctg aaaacgctgg
     3301 ggcttttgcg ttgtgaaaag ctcaataaat caaagcgcga tatgaccaat ttctacacga
     3361 tcaactacgg gagcgagctt ttagatgatg gcaaattgag cgaatccatc ggttcaaaat
     3421 gcgccgctcc atcaggtcaa aatgacacga tggaagaggt caaaatgaaa cgctccattg
     3481 gttcaaaacg atccaatgtc atcgggtcaa aatggcctga tgatcttaca gagaatacaa
     3541 cagagattac tacagagaat aaaaacactt ttcgtccgga agcttcgcaa ccggacccgc
     3601 agacgactga acaggatttt ttaacccgga actccgacgc ggttgtgttt agtgcgaaaa
     3661 aacgccagtg gggtagcagg gaggatttgg cgtgtgcgca gtggatctgg gggcggatcg
     3721 tgggcctcta cgaacaggca gccagtgatg atggcgagat catgagacca aaagagccta
     3781 actggactgt ctgggccaat gacgtgcgca caatgcggat gctggatggc agaagccaca
     3841 gacaaatttg cgaaatgttt ggtcgggttc agcgagatcc attctgggta aaaaacatca
     3901 tgagcccgtc aaagctccgc gaaaaatggg acgaactggt catccgcctg ggacgttcac
     3961 ctgtacagcg ttgtgttaat catatttctg aaccggatac agaaattccg cctggtttca
     4021 gaggataagt gttgatttca ggtcatgagg taattttaag ggggacttgt ggcaaaagtt
     4081 tttacacaag aagagcggga aaaaattaaa gggcaggtgg tggaactcgt gcgccagagc
     4141 ggtcgtgaga cgttacggca actggaagct aaaacaggtg cgactagata tctgatgagc
     4201 gttcttgcca gagagcaggt agccagtggc gatgtataca actccggcta cgggttattc
     4261 ccgtctgaac aggcgcgtaa ggactggcaa aacgcccgca aaaaactctc gagggcaaag
     4321 gtgaagaaac ctgctgtggt tgatccggac cttatctggt cattacctga cggagaaata
     4381 cgccgctacg acagtcgtct aaacataatc tgtcgcgagt gccggaagag cgaagttatg
     4441 cagcgtgttc tgacgtttta tcaggggaat tatcgggagg tgatgctgtg agtacatcgg
     4501 ctaccattct cgacatgtgc tgtggcagtc gcatgctctg gttcgataag aatgacgaat
     4561 ggtcgatatt tagcgatatc aggaaagagg aacattcgct atgtgatgga cgtcacccga
     4621 ttattaatcc cgacattatc gctgattttc gtgcgctgcc gtggttgtct gctttgcatc
     4681 aaaagcagac attaccagat ttagacatct gtttccgata gcccgttctg atgctacact
     4741 ttttgctatt ttcatgaacc tcataaacac atttatgact gttgctgatt tcaaacgccc
     4801 caaattggag ctcccaaacg gggcaaaaaa actattactg cactcttgct gtgctccatg
     4861 ttccggtgaa gtgatggagg cgcttcaggc ctcgggaatc gactacacca tctttttcta
     4921 caacccgaac attcatcctc agaaagagta tttaattcgt aaggatgaga atattcgctt
     4981 tgctaaacaa cacggcgtgc catttattga tgctgattac gacacagaca actggtttga
     5041 gcgagccaaa ggaatggaat gggagcctga acgagggatc cgttgtacca tgtgttttga
     5101 catgcgtttt gagcggactg cactgtatgc cgctgaaaat ggtttcagtg tgatcagtag
     5161 ctcactgggc atttcacgct ggaaaaatat gcagcaggtt aacgactgtg gacggcgagc
     5221 cgtcgcgcat tatccgggca tggtgtactg ggattataac tggcgaaaac agggcggctc
     5281 ttcccgcatg attgaaatca gcaagcgcga acaattctat cagcaggaat attgtggctg
     5341 cgtgtattca ctgcgcgata ccaatctaca ccgcaaatct cagggacgcc ctctaatcaa
     5401 aattggtcaa ctgcactacg gtaaggaagg acaggaatga ttttatatta tgccaacgga
     5461 gcgtagcagt gtttctatat atagtacgcc taccttaatt ttcaaataat catgattaat
     5521 gagcaggtca tcagtgcaag caatgacctg ctcatcttgt tgaaattaga ggaaatcttg
     5581 cagccatgat atggcttaaa ataacgtttg attattttac ctgctgtttg tccctggttt
     5641 aaagtcggtt gtctgatttg attacgcgca actgacgctt acgtattgaa gtaacgagca
     5701 cgaagacaca tatttatcag tgcctgtata cgtcaagagc aggagattta tgtgctggtt
     5761 ctcaaatgca cgctggctat tgtggctgta atggcaattt attgtcttgt tattgttctt
     5821 atggatcacc tttctgattg atttcatatt ggcgaggtaa cggtagttaa gtagaatggc
     5881 tgcgggtgct tgaggctatc tgcctcgggc atgaacacca acggcagata gagaaaagcc
     5941 ccagttaaca ttacgcgtcc tgcaagacgc ttaacattaa tctgaggcca atttcatgct
     6001 agacacatgt aggttagcct cttacgtgcc gaaaggcaag gagaagcagg ctatgaagca
     6061 gcaaaaggcg atgttaatcg ccctgatcgt catctgttta accgtcatag tgacggcact
     6121 ggtaacgagg aaagacctct gcgaggtacg aatccgaacc ggccagacgg aggtcgctgt
     6181 cttcacagct tacgaacctg aggagtaaga gacccggcga ggcagaaatc cctcgccacc
     6241 tctgatgtgg caggcatcct caacgcaccc gcacttaacc cgcttcggcg ggtttttgtt
     6301 tttattttca acgcgtttga agttctggac ggtgccggaa tagaatcaaa aatacttaag
     6361 tagcgcgcag ggataagagg gatggtccct taaaggggag agctaattat ccggaaggat
     6421 tctgatgatg aacatcgaag aactgcgtaa aattttttgt gaagatggcc tctatgctgt
     6481 gtgcgttgaa aatggaaaca ttgttagtca ttaccgcatt ctgtgtttgc gaaagaatgg
     6541 ggctgcgtta attaattttg tggatgctcg ggtcacggac ggatttatct tgcgcgaagg
     6601 tgagtttgtc acttcattac aggcactgaa agagatcgga ataaaagcag gcttttcagc
     6661 ttttgcagaa gaataaactc atctacaatc ttgcgcgggg ctgaactccc gctgagtaac
     6721 accgtgccac cggagaaaac cgatggcacg caacgtaaaa tattacaatt ctgataattc
     6781 gcccgttctt gtctgcacgc acgagcggta ttctcacgca ttcaagtctg aatggttcca
     6841 acaccctcca tgcactgaag agcaggctga atggataatt cagtgttacc gcaggcgcgg
     6901 atacgaggtt aagaaagccc ttagcctcga ctaccgtcac tggataatct cagtcagatt
     6961 gccttactcc gaacgcccac cacgtgcgtc ccgcactttc cagcaacgga tctggaggta
     7021 acgtgcgggt attacttaga cctgttctgg tgcctgagct tgggctggtg gtccttaagc
     7081 cgggccgtga atccatacag atatttcata atcctcgagt gctggtggaa ccggaaccaa
     7141 aaagcatgcg taatctgcca tccggagtcg ttcctgccgt tcgccagccg ctggcggaag
     7201 acaaaacatt gctgccgttt tttagtaacg aacgggtgat tcgtgctgct ggcggcgttg
     7261 gcgcattgtc tgactggcta ttacgtcatg ttacatcctg ccagtggcct aatggcgatt
     7321 accatcactc tgaaacagtc attcaccgtt atggtaccgg cgcaatggtg ttgtgttggc
     7381 actgcgacaa ccaactgcgt gaccagacat cggaatcact ggatctgctt gctcaacaaa
     7441 atctgacagc atgggtgatt gacgtcatcc gtcacgcaat aagcggtacg caggaacgtg
     7501 aattatctct ggctgaatta tcctggtggg cggtccgcaa tcaggtggcg gacgcgctac
     7561 cggaagcggt attacgtcgt tcgctgggat taccagcgga aaaaatctgc tcggtgtacc
     7621 gcgagagcga catcgtaccg ggagagcaga ccgccaccag catattgaaa caacgcacaa
     7681 aaaatcttgc accgttgcct tacgcccacc agcaacaaaa actaccacag gaaaagacgg
     7741 tggtaagcat caccgttgat ccagagtctc cggaatcttt catgaagctg cctaaacgtc
     7801 gccgctgggt taaggagaaa tacacacgtt gggttaagac acagccgtgt gcttgctgcg
     7861 gtatgccagc cgacgatccg catcatctga ttggtcacgg gcagggcgga atgggaacaa
     7921 aagcacatga tctctttgtg ttgcctttgt gcagaaagca tcacaacgag ctgcataggg
     7981 atacagtggc atttgaagag aagtatggct cccaactgga gctgatattt cgttttatcg
     8041 atcgcgcgct ggcaattggc gtactggcgt aagtggagaa cgagcatgaa ccttgaagcc
     8101 ttaccaaaat attactcccc aaaatctcca aaattgagcg atgactctcc agcgacaggc
     8161 accggttgtt taacaattac ggatgtaatg gcagcgcagg ggatggtgca gtcgaaagca
     8221 ccacttgggt tggccttatt tctggcaaaa gttggtgttc aggaccctca gtttgcgatt
     8281 gaaggcctgc taaattacgc gatggcactg gataacccga cattgaacaa attgagtgaa
     8341 gaaatccggt tacagattat tccttacctc gtgagttttg cctttgctga ttactccagg
     8401 tctgcggcaa gtaaggctcg ctgtgagcat tgttcaggta cgggatttta taatgtattg
     8461 cgcgaagtgg tgaaacacta cagacgcggg gaatctgtaa tcaaggaaga atgggtgaag
     8521 gaactatgtc agcattgcca tggtaagggc gaagtcagca cagcgtgcag agggtgtaag
     8581 ggtaaaggga ttgttctgga tgaaaaaaga acccggtttc atggcgtacc ggtatataag
     8641 atttgtgggc gttgtaatgg aaaccggttt agtcgtttac cgaccacgct ggcacgacgt
     8701 catgtccaga agctggtacc agacctgacc gattatcagt ggtataaggg gtatgcggac
     8761 gtcattgata aactggtaac aaagtgctgg caggaagaag catacgcgga agcgcaattg
     8821 aggaaggtga cgagataaat gatttttgct gaagatggcg acatgatgtt tgcatttttc
     8881 aaaaaacatg gataagattt tctcaacgat gggctttgtg tatccgacgt ttagaaaaaa
     8941 gtagaaaacc cgctgataag cgggtttttg tgctttaaat ggggcaatag agatattgaa
     9001 tctcatcccg ggataaacat tgggagttga aggtccacgc gaaccattta tccggcaaaa
     9061 ttccacgcgt aatcctgtgg taatttcttc tgcatctcga agattgagag ctgaaacgtg
     9121 aagctgggca tcgatacgcc atcggatggg aatataagac ctttgctgct tttgtagtca
     9181 aagtttttga caattcctgt cattttaggg gacagaaaaa ctccttaata ctgataacct
     9241 ggtgcaccat acacacgttc ctggagaaaa ctactttgtt gatagggttg aaggtgtctg
     9301 gatgtctaaa ataaacattg cttcatatgt tcaactatga gttaatgact gcgtcggttt
     9361 gaagaacaga cgatatacga agtagtttac taaagcagtt ctcatttcag gtgttattca
     9421 cttattcctt ctttgagtct ctccaattaa gtacgaagtc gtttctgtta tgcaaaccat
     9481 ttatgccgaa aggctcaagt taaggaatgt agaatgtcaa ataaaatgac tggtttagta
     9541 aaatggttta acgctgataa aggtttcggc tttatttctc ctgttgatgg tagtaaagat
     9601 gtgtttgtgc atttttctgc gattcagaat gataattatc gaaccttatt tgaaggtcaa
     9661 aaggttacct tctctataga gagtggtgct aaaggtcctg cagcagtaaa tgtcatcatt
     9721 actgattaaa attcatcgct cgtctgtata cgataacgaa gaaggctgat gcctgagtag
     9781 agatacggac agagtagtga atatt
//
LOCUS       DAZGVQ010000183         9765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000183 DAZGVQ010000000
VERSION     DAZGVQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.184"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(133..285)
                     /locus_tag="ACHLAG_004464"
                     /pseudo
     CDS             complement(133..285)
                     /locus_tag="ACHLAG_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
     gene            622..2178
                     /locus_tag="ACHLAG_004465"
     CDS             622..2178
                     /locus_tag="ACHLAG_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012884439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFP0657580.1"
                     /translation="MHDYLLFLLGLTPLLAMIILILKVKMPIHYSVLSTLLLTLILGE
                     VFWQTPVMDMGSAVVYGAIKGLWPIVIVILAAIYSYNLMNATKSMDILRNVLASVSDD
                     KRIQVLLISWCFGGFLEAAAGYGTAVAIPIGILIALGFNPLKAAIASLVANTVPTAFG
                     AVGIPVSVLAEQVNLPVTTLSGTIILQLALFNILLPFVIVTIIGGGMKAIRGVGLITL
                     MCGISTLIPQYLVAVNLGAELPAFAGSLVSLIVIVFMAKNRKEKTEPEFLIESSADSA
                     SALSYSRGALLRACSIYILIFLFILLCSPLFPTIKSAVSQIASSLTFSLSNGSNLSMK
                     IEWIATPGVLIIIASVIGGLIQKASLSCLLNVFVSTVKQLKNSIIAISAIVAMATVMD
                     VCGLIGTLAQSLVDLTGGAYLFIAPVIGALGTFVTGSDTNSNILFGKLQTTAAEKLHV
                     DPLWLAAANTSGATGGKMISPQSIAIAVSATRMDGQGSVIMSGTLKYCFAYIVILGLK
                     VGLVYHLFMA"
     gene            2228..2947
                     /locus_tag="ACHLAG_004466"
     CDS             2228..2947
                     /locus_tag="ACHLAG_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFP0657581.1"
                     /translation="MNVNFFVTCIGDAFKSRMAQKSVLLLEQLGCNVHFPEKQGCCGQ
                     PAINGGYVRDAIPGMKNLISSLEENDWPVVSPAGSCTYTIKNYARYLSEEPEWAARAE
                     KVAERLHDLTSFIVNHLGITDVGAKLNGRAVYHPSCSLSRKLGVTREPLLLLENVQGL
                     ELLPFHAQETCCGFGGTFSVKMAEISGEMVKEKVGHIMDVKPDFLIGADASCLLNIGG
                     RLNREGHPVKVMHIAEVLMHR"
     gene            2958..4373
                     /locus_tag="ACHLAG_004467"
     CDS             2958..4373
                     /locus_tag="ACHLAG_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFP0657582.1"
                     /translation="MSLKTSDIRFKERIRIQIQDPIMRKAVANAQERIGTNRQKMVDE
                     LGHWEEWRSRAEQIRRHVLENLDAYLYQLSEKVTANGGHVYFARTREDATSYILKVAK
                     EKNARKVVKSKSMVTEEIGMNAVLQKAGIPVIETDLGEYILQLAHEPPSHVVVPAIHK
                     DRHQIRRVLHEHLGYDGPETPEAMTLFIREKIRQDFLSAEVGVTGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFQEVDVLITMLARSAVGARLTGYNTWLTG
                     PREEGHVDGPEEFHLVIVDNGRSEILGSEFSDVLRCIRCGACINTCPAYRQIGGHGYG
                     SIYPGPIGAVISPLLGGYEDFKDLPYACSLCTACNGVCPVKIPLATLIQKHRQVMAES
                     EITPLTERRITRAFNYINSHPALWKVGMVAGAKTARLFIRKGKTPISVGAIKEWTEAR
                     DLPSPDGESFRSWFKRHKKEG"
     gene            4377..5075
                     /locus_tag="ACHLAG_004468"
     CDS             4377..5075
                     /locus_tag="ACHLAG_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000431499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFP0657583.1"
                     /translation="MENREEFLVAIARKLGRPVRHIPEAMPEPVNTLATTRLTELTSD
                     QRCEAFIKFASEVMLAECVLVSPENAPSTALDICWKFGSGPVIISNDQRLVDTGITPL
                     LQKEMDAALWDPEKGDENIHLAEKAKTGVVFAEYGLTESGGIVLFSAPERGRSVSLLP
                     EASLFVLRKSTILPRVAQLAQKLHDMAQEGQRMPSCINLIGGPSSTADIELIKVVGVH
                     GPVHATYLIIDDCD"
     gene            complement(5480..8734)
                     /locus_tag="ACHLAG_004469"
     CDS             complement(5480..8734)
                     /locus_tag="ACHLAG_004469"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFP0657584.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALRQIFDAEDVLLQQAAELPPSYINPPLNLIKIVNQLKISGKD
                     NQSRIPDGILYINGLPLVVFEFKSAVREQDASIGNAWRQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKARHEVKICCRYPQYYAARKLYYSIKQARKPFGNGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTILPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERSNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTESVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDRLKALA
                     EDFAKHYEKRVAEGSTVKGKAMFVCASREIAWDFYRQLKAIRPAWFEVKQAPDGVFLT
                     EQEQKELPPSEMVKMVMTRGKDDDEALYDLLGTKEYRKELDKQFKNAKSNFKIAIVVD
                     MWLTGFDVPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQAL
                     AMYSRIDATNFEDIQQSVTEVKNHLDLLGQVFYDFDSRDYFSGEPQAQLSCLNRAAEF
                     VLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDHIHYYLAVRSIVFKLTKGDAP
                     DVTQMNARVREMIAEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATKIQ
                     LLQKLLEKAISDFRKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTD
                     IIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNT
                     SKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKKNHMS"
     gene            complement(8829..>9765)
                     /locus_tag="ACHLAG_004470"
     CDS             complement(8829..>9765)
                     /locus_tag="ACHLAG_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP0657585.1"
                     /translation="AKTNTLIFSKLKSNEVINKSLEQMSQTLFKSWFVDFDPVIYNAL
                     DAGNPIPEALQSRAELRQKVRNSTDFKPLPAEIRSLFPSEFEETELGWVPKGWSIVRT
                     EDIALKIGMGPFGSNIKVSTFVNAGVPIISGQHLKALLLIDGDNNFITPEHAEKLKNS
                     AVYRKDIIFTHAGNIGQVSLIPEDSEYDRYIISQRQFFLRVNESKSSPYYLIHYFRSE
                     KGQHALLSNASQVGVPSIAQPSTHLKNISFLNPPMVLLKEFEKFSTPLFHRFSKNRKC
                     GVSLTALRNTLLPKLISGELSLEDLPDLSTDTEAA"
BASE COUNT         2582 a         2124 c         2267 g         2792 t
ORIGIN      
        1 tttataaaac tttaaattaa ttgcaaaatg gcatctgaat cgatcatagt ttggcattcg
       61 aatcgatcat gatttggctc tcttgctccg gctttatcca tttcctctca gacgacagtc
      121 agtgtcctgc tctgactgaa cctctgcacc gttgttacgt accagcgttt gtttatctat
      181 tagagtcact tcagtaggca catcagatgc caacgcaaca ttccctgcca taagcgcagc
      241 cagaacgcct gttacaacta aactattcct ggtgatattg ctcattctct ttctccattt
      301 gtcgaggatt tgggcgttga ttttcgacga gctccagttg caactccagt cttaaggtta
      361 tatcgggata gcacacaagt gttacacaca taaacaaaaa atacccaaaa agtattattc
      421 cataacattt attcatggaa gataacaatg aaacgattca gctatatttt ttaaactaaa
      481 aaatacttta taagaacatc ccaattttgg aggcgatact cattctcccc ctgataacta
      541 aacactctcg tccgacaggg cgtggaaact atcaataacc acagtaatga tgctgacttt
      601 gttttcgttt aaggaaatga catgcacgat tacctgcttt ttttactggg acttaccccc
      661 cttctggcga tgatcatttt gattctgaaa gtcaaaatgc ccatacacta ttctgtgctt
      721 agcactctcc tgctcacgct aatccttgga gaggttttct ggcagacgcc agttatggat
      781 atgggatctg ctgtggtata cggcgcaatc aaaggtttgt ggccgattgt gattgtcatc
      841 cttgctgcca tttacagcta taacctgatg aatgcgacaa aaagcatgga tattctgaga
      901 aatgttctgg cgagcgttag tgacgataaa cgcatccagg ttcttcttat ctcatggtgt
      961 tttggcggat ttcttgaggc ggccgccgga tacggtacag cggtagcaat ccccataggt
     1021 atactcattg cactgggctt taacccacta aaagcagcga ttgcatcact ggttgctaat
     1081 acagtcccta cagcgtttgg ggcagtcggt atacctgtct ccgtgcttgc tgaacaagtt
     1141 aatttacctg taaccacact cagcgggacg attattcttc agctggcact gttcaacatt
     1201 ctgctcccct tcgttatcgt cacgattatc ggtggaggta tgaaagctat acgaggcgta
     1261 gggttaatta ccctgatgtg cggtattagc acattgatac ctcagtatct ggtggctgtt
     1321 aatcttggcg cagagctccc ggctttcgca ggaagtcttg tcagtctgat cgttatagtc
     1381 tttatggcaa aaaaccgtaa agaaaaaaca gaacctgaat ttcttattga gagttcagca
     1441 gatagcgcca gcgcactttc atactctcgt ggggcgttgt tacgtgcgtg ctcaatctac
     1501 attctgatct ttctgttcat tctgctttgc tctccactgt tcccgacgat aaaaagtgca
     1561 gtgtctcaga ttgcctcgtc actcaccttc tctctgagta acggcagcaa tctgtcgatg
     1621 aaaattgaat ggatcgcaac ccccggtgtt ctgattatca ttgccagtgt tattggcggc
     1681 ttgatacaga aagcatccct gtcatgcctg ttaaacgtat ttgtcagcac ggtaaaacaa
     1741 cttaaaaact ccattattgc catttctgcg attgttgcca tggcaacggt aatggatgtc
     1801 tgtgggttga ttggcactct ggcacagagc cttgtggatc tgactggtgg ggcctacctc
     1861 ttcattgctc cggttatcgg cgctcttggt acatttgtta ctggtagtga cacaaactcc
     1921 aacattctgt ttggtaaatt gcagaccact gcggctgaaa aattgcatgt ggacccgctc
     1981 tggctggctg ccgcaaatac atctggcgca acaggcggca aaatgatttc tcctcagagt
     2041 attgctatcg ctgtttcagc cacccgtatg gatggtcagg gcagcgtaat catgtcagga
     2101 acactgaagt actgctttgc ttatattgtt attcttggac tgaaagtagg ccttgtctac
     2161 cacctgttca tggcctgaga aaaaacaaca aaaggacgtg gaatattaat caacaggaga
     2221 aatttctgtg aatgttaact ttttcgtcac ctgcattggt gatgcgttta aatcccggat
     2281 ggcacaaaag agtgtgctgt tactggagca attgggttgc aatgtccatt tccccgaaaa
     2341 gcagggatgt tgtggtcagc cagcgataaa tggcggttat gttcgtgatg ccatcccggg
     2401 catgaaaaat ctcatatctt cgctggaaga gaatgactgg ccggtagtat ctcctgccgg
     2461 gtcatgcact tacacgataa aaaattacgc tcgctatctc agtgaagaac ctgagtgggc
     2521 ggcacgagct gaaaaagtgg ctgaacgcct gcatgatttg acatcattta tagtaaatca
     2581 tcttggcatc acagatgttg gggcaaaact caatggtcgc gctgtgtacc atccctcctg
     2641 cagcctttcc cgtaagctgg gtgtaaccag agagccgctt ttgctacttg agaatgtaca
     2701 ggggcttgag ctactgccgt ttcacgctca ggaaacgtgc tgcgggtttg gcggcacatt
     2761 ctctgtaaaa atggcggaga tctctggtga aatggtgaaa gagaaagtcg ggcatattat
     2821 ggatgttaaa cctgattttc ttatcggggc tgacgccagt tgcctgctaa acatcggcgg
     2881 tcggcttaac cgtgaagggc atcctgtaaa agtgatgcat attgctgaag tcctgatgca
     2941 cagatgaggt aaacagaatg tctttgaaaa caagtgatat caggtttaaa gagcgtatcc
     3001 gtattcagat tcaggacccg atcatgcgaa aagctgtggc taacgcacag gagcgcatag
     3061 ggactaatcg acagaaaatg gttgatgaac tgggacactg ggaggaatgg cgttcacgag
     3121 ctgaacagat tcgtcggcat gttctggaaa atcttgatgc ttatttgtat caattgtccg
     3181 aaaaggttac tgcgaatggc gggcatgtat attttgcccg taccagagag gatgcaacca
     3241 gttacatact gaaggtggcg aaagaaaaaa acgccaggaa ggttgttaaa tccaaatcaa
     3301 tggtgactga ggaaattggc atgaatgccg ttcttcagaa agccgggata cccgtgattg
     3361 aaacggacct tggggaatat attcttcagc tagcacatga gccaccatcg catgtggttg
     3421 tgccggcaat acataaagat cgccatcaaa tccgcagagt tttacatgaa catctggggt
     3481 atgacgggcc tgaaactcca gaagcaatga cattgttcat ccgtgagaaa atcagacaag
     3541 attttctgag tgctgaagtt ggcgtaacgg ggtgtaactt tgccgttgca gaaacagggt
     3601 cagtctgcct tgttacaaac gaaggcaacg cacggatgtg caccacatta cctaaaacac
     3661 atattgctgt tatgggtatg gagcgtattg ctccgacatt ccaggaggtg gatgttctga
     3721 ttaccatgct tgcaaggagt gctgtcgggg cgagactgac aggttataac acctggctta
     3781 caggccccag agaagaagga catgttgatg gtccggaaga gttccatctc gttattgttg
     3841 acaatgggcg ttcagagatt ctcggctcag aattcagcga tgtattgcga tgtatccgct
     3901 gcggtgcctg cattaatacc tgcccggctt atcgtcagat tggtgggcat ggttatggtt
     3961 caatctaccc ggggccaatc ggcgcggtta tttccccatt gcttggtgga tatgaagact
     4021 ttaaggattt accctatgcc tgctcattgt gcactgcctg caatggggta tgtcctgtaa
     4081 aaatacctct ggcaacgcta atacaaaaac atcgccaggt tatggcagag tctgaaatca
     4141 caccattaac ggaacgccgt ataaccagag cattcaatta cataaacagc catccggcgt
     4201 tgtggaaggt aggtatggtt gctggggcca aaacagcccg tctgttcatc aggaaaggaa
     4261 aaacgccgat tagtgttgga gcgataaaag aatggactga agccagagac ctgccatcac
     4321 ctgatggaga aagcttccgt agttggttca agcgacataa gaaggagggg taatttatgg
     4381 aaaacagaga ggaatttctc gttgcgatcg ccagaaagct tggcagacca gtaagacata
     4441 ttcccgaagc aatgccggag cctgtaaata cgctggctac gacacgttta acagagttga
     4501 catcagacca acgctgtgag gcgtttatta aatttgcctc ggaagtgatg cttgccgagt
     4561 gtgttctggt gtcgcctgag aacgcaccat caacagcctt agacatctgt tggaagttcg
     4621 gttccgggcc agtcattatc agcaacgatc aacgactggt cgatacaggg attacgccat
     4681 tgctacaaaa agaaatggac gctgctctgt gggatccgga gaagggcgat gaaaatatcc
     4741 atcttgctga aaaggcaaaa actggtgtcg ttttcgctga gtatgggctg acagaatcag
     4801 gaggcattgt cttattctcc gcaccagaac gtgggcgctc agtaagcctt ttgcctgaag
     4861 catcattatt tgttttacgt aaaagcacca ttctccccag agtggcgcag cttgcgcaaa
     4921 aacttcatga tatggctcag gaaggacaac ggatgccgtc ctgtataaat ctgattggag
     4981 gcccaagttc tactgcagat atagaactga tcaaagttgt tggcgtacat ggtcctgtac
     5041 atgcaaccta tttgatcatt gatgattgcg attaacgatt gctaataaaa aaatggcctt
     5101 aaggtgatat ttcgtcttgt gaaaaggcaa caatagacta aaggttaggc ataattgtgt
     5161 ttctttatgc ctccgcagca atttgtggag gcttttttgc ttactccaaa cagtttggct
     5221 tcttgaaaaa gtcatatccc aaacctattg acgcttataa aaaagtaaag aatggtgagt
     5281 aagcgtaaac tgaccaccgt atgtagccat gatgccagta ttggtaacgt aggtgcaccc
     5341 ctatctattt ttgtagaacc ggagtaaaca gaccagatag tttaaatgag ctggttatgc
     5401 gtaacataat ttatctattt tggctcttac aaaatagata aagaaggagt atctgatgag
     5461 atactccatt atgacagact caactcatgt gatttttctt aaagttctct gcttgcgcca
     5521 gtacccccat ataaacatca tcattcgcta ctggcgggaa cttatgcttg tgtagaagca
     5581 gaataagttc aactttcagt ttcgctttaa tatcatcgcg tttactccag tcaggatatt
     5641 tcgatgtgtt gtcaaccacg cttttcatct cttttgccag cgacagcatt ttttcatcgt
     5701 cataggtgaa ctgatattta tcgcgcatat gagcaagaat gtcgaagaac gctttttctt
     5761 caatatcaat acctaaatcg gcccaggtgc ccatttctgt tttaatatca tagataatat
     5821 cggtcatttc ctgactgaat gtatcgaatt cttcaccgtt gagtacatca tcttctcgcc
     5881 gctcattata acgatctata atagcctgga agcggcgggt gaagttaatc ccttgcaact
     5941 ggttcacttt cctgaagtcg ctgatcgctt tttccagtaa tttttgtaat agctggatct
     6001 tcgttgccgg aagtttgatc ttgttaattc gcgccagata atcttcgtca aaaatatcga
     6061 tggattccgc ttttttatcg ccaagaaaat aaatttcttc tacgccatca gcttttagcg
     6121 cttctgcaat catttcacga acgcgtgcat tcatctgggt aacatccggt gcgtcacctt
     6181 tcgtcagttt gaaaacaatt gaacgaacag caagataata gtgaatatga tcacgttctg
     6241 tctgtgatag tgcttcactg ccgcagcaga cgtcgtaggc ggctttcatg cgtttaacca
     6301 gtcccataaa acgacgttca actttctggg tacgcagaac gaattccgcc gcgcggttga
     6361 ggcaggataa ttgcgcttgt ggctcaccac taaaataatc ccgactgtca aagtcgtaaa
     6421 agacttgccc caacaaatcg agatggtttt taacttcagt caccgattgc tgaatatctt
     6481 caaagttggt ggcatcaatg cgggaataca ttgccagtgc ctggttcatc tgacttttaa
     6541 tgccgatgta gtccaccact aaccctttgc ttttgccttc cagtttacgg ttaacgcgag
     6601 aaatagtctg gataaggtta tgtttttgta agggcttatc aatatagatg gtatccagtt
     6661 caggaacatc aaaaccggtc agccacatat caacaacaat ggcaattttg aaattcgatt
     6721 tagcgttttt gaactgctta tccagctctt tgcgatattc ttttgtgccc agtaaatcat
     6781 aaagcgcctc gtcgtcatct ttaccgcgcg tcatgaccat cttcaccatt tcagaaggcg
     6841 gtaactcttt ttgctcctgt tctgtcagga agacgccatc gggggcttgc ttcacttcaa
     6901 accaggcagg gcgaatagct ttaagctggc ggtagaaatc ccaggcaatt tcacggctgg
     6961 cacaaacaaa catggctttg ccttttacgg tggaaccttc ggctacgcgt ttttcataat
     7021 gtttggcaaa atcttccgcg agggctttta atcgatcttc atcacccaga accgcattca
     7081 tggttgcggt ggcttttttg ctttcgtcga tttgccactc attggtgcct gcgtttgcgc
     7141 actcttcgta atacttttcg acttcttcca gtttgctgga gtccaggatc actttagccg
     7201 cacggccttc gtacacgatg cgtacagtga tttcatcctg aacggattcg gtcatggtgt
     7261 aactgtcgat cacctcaccg aagacatcga gcgtcgcgtc aatcggtgtg ccggtaaagc
     7321 caacataggt ggcgtttggc agtgaatcgt gcaggtattt cgcaaagcca tacgttttgc
     7381 gcacttttcc gctttcttta tcgacgatga ctttctggtc gaggttaacc tggctgcgat
     7441 gtgcttcgtc cgagatgcaa atgatattgc tgcgttccga aaggagttcg gtgtcttcgg
     7501 tgaatttatg gatcgttgtc aggaatacac cgccactatt gcgtcccgcc agttgattac
     7561 gcaaatcttc acggctggta acgggaagga tggtgtcgtc accaatgtaa tttttggcgt
     7621 tgcacatttg cgcagaaagc tgatcgtcca gatcggtgcg gtcggtgatc aaaacaatgg
     7681 tcgggctggc aaactctacg ctcttcatca aaagacgcgt taaaaattgc atggtgtaac
     7741 ttttgccaca gcccgttgcg ccaaagtaag tgccgccttt accgttaccg aaaggtttac
     7801 gcgcttgctt gatgctgtaa tagagtttgc gggcggcata gtactgcgga tatcggcagc
     7861 aaatttttac ttcgtgcctg gctttatccg ggaagcagat aaagttttta attacatcca
     7921 gcagacgtac cggatgaaac agcccctgaa tcattgagtg caatgatgga attccatcct
     7981 gttcacggtt ttcattaccg gtgacttttc gccatgagta aaaatattca tagggcgcaa
     8041 acaggttgcc catccggtta ttaactccat cgctaataat gcagagcgcg ttgtagataa
     8101 acagttgcgg aatatcccgg cgatagcgtt tgcagagttg tctccaggca ttgccaatac
     8161 tagcatcctg ctcgcgcacc gcacttttaa attcaaagac gaccagtggc agaccgttga
     8221 tatagagaat gccgtcagga atacgactct gattatcttt gccggagatt ttaagctgat
     8281 taacaatctt aatcaggtta agcggcggat taatatagga gggcgggagt tccgcagcct
     8341 gttgcaacag gacatcttcg gcgtcaaata tctggcgcag tgcggcaggt agatgccggg
     8401 tgtcgagcaa ttcaatgtag agatcttttt gttgccgatc gtcgcgtttg aacagaaaac
     8461 catttgccag ccagcgacaa aatgttttgt tgctttcata aagatcggaa gccggaagcg
     8521 tggtgaactg tttgatcagt cgctgaatct cttcttcagt aatgccatca ggctggtagc
     8581 gtgccgctaa ataatgacgg agatcgtctt cgataatgac ctgatcgaga ctcgaacgtg
     8641 ggacattatc gccgatcaga tgttgatacc cctgatcctg taacagttca atgatcgctt
     8701 gttctaattt tgcttcactg aaagagagac tcatatcgcg ctccaaccct ggctaatttc
     8761 tggtaagcat tcctgtatca gtagaaaacc ttaccagaaa gcccctgatt ttacaggggc
     8821 aaaatgcgtt atgcggcttc tgtatcggtg ctgagatccg gaagatcttc cagggatagc
     8881 tcaccggaga taagtttcgg gagcagggtg ttgcggaggg ctgttagtga gactccacat
     8941 tttctatttt tactaaagcg atggaataaa ggggtgctaa atttttcaaa ctctttaagc
     9001 aaaaccattg ggggatttag gaatgatata tttttcaaat gtgttgaagg ctgagcaatt
     9061 gaaggaacac caacctgaga ggcgttagaa agcagagcat gttgtccttt ttctgaccta
     9121 aaataatgaa tcaaatagta cggcgatgat tttgattcat ttacgcgtaa gaaaaattga
     9181 cgttgggaaa ttatatatct gtcatattca gaatcttcag gaattaaaga aacttggcca
     9241 atattacctg catgtgtaaa aattatgtct tttctatata cagcagagtt tttgagcttt
     9301 tcagcatgct ctggagtaat gaaattatta tccccatcga taagaaggag ggctttcaga
     9361 tgttggccgc ttataattgg tacaccagca ttaacaaatg tggatacttt aatattggaa
     9421 ccaaatggtc ccattcctat tttcaatgca atatcttcag ttcgaacaat actccatcct
     9481 ttcggcaccc aacccaactc cgtttcttca aattcacttg ggaaaagcga acggatttcc
     9541 gccggaagcg gtttaaaatc tgtactatta cgtacttttt gacgtaattc ggcacgagat
     9601 tgcagagctt ccgggattgg atttcctgca tccagagcgt tataaatcac cggatcaaaa
     9661 tccacaaacc aggatttgaa gagagtttgg gacatttgtt ctaatgactt atttattact
     9721 tcattggatt ttaacttact aaatataaga gtgtttgttt ttgcg
//
LOCUS       DAZGVQ010000184         9744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000184 DAZGVQ010000000
VERSION     DAZGVQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.185"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            483..2411
                     /gene="thrS"
                     /locus_tag="ACHLAG_004471"
     CDS             483..2411
                     /gene="thrS"
                     /locus_tag="ACHLAG_004471"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP0657586.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2415..2957
                     /gene="infC"
                     /locus_tag="ACHLAG_004472"
     CDS             2415..2957
                     /gene="infC"
                     /locus_tag="ACHLAG_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFP0657587.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3054..3251
                     /gene="rpmI"
                     /locus_tag="ACHLAG_004473"
     CDS             3054..3251
                     /gene="rpmI"
                     /locus_tag="ACHLAG_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFP0657588.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3304..3660
                     /gene="rplT"
                     /locus_tag="ACHLAG_004474"
     CDS             3304..3660
                     /gene="rplT"
                     /locus_tag="ACHLAG_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFP0657589.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    3777..3901
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3783..3827
                     /gene="pheM"
                     /locus_tag="ACHLAG_004475"
     CDS             3783..3827
                     /gene="pheM"
                     /locus_tag="ACHLAG_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFP0657590.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4111..5094
                     /gene="pheS"
                     /locus_tag="ACHLAG_004476"
     CDS             4111..5094
                     /gene="pheS"
                     /locus_tag="ACHLAG_004476"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFP0657591.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5109..7496
                     /gene="pheT"
                     /locus_tag="ACHLAG_004477"
     CDS             5109..7496
                     /gene="pheT"
                     /locus_tag="ACHLAG_004477"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFP0657592.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVTVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7501..7800
                     /gene="ihfA"
                     /locus_tag="ACHLAG_004478"
     CDS             7501..7800
                     /gene="ihfA"
                     /locus_tag="ACHLAG_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFP0657593.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(8042..8182)
                     /locus_tag="ACHLAG_004479"
     CDS             complement(8042..8182)
                     /locus_tag="ACHLAG_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0657594.1"
                     /translation="MSQKSLMAITFCVTAILIIWMLHGSLCEIRMNIWGAEFAAFLQC
                     KQ"
     gene            complement(8312..8614)
                     /locus_tag="ACHLAG_004480"
     CDS             complement(8312..8614)
                     /locus_tag="ACHLAG_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFP0657595.1"
                     /translation="MFHCPFCGASARIRTSKAANDTGTIRYKYYQCNNLECSATFSTM
                     ESVHRIIGQPQLIFHDKDIPRETMRKSHQGRSQLTLQLEDCGMALKPVHYARVPFG"
     gene            complement(8659..>9744)
                     /locus_tag="ACHLAG_004481"
     CDS             complement(8659..>9744)
                     /locus_tag="ACHLAG_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFP0657596.1"
                     /translation="KELVKMPDFSVSLDREQDKNTLQELNNRVISLSMTDNRGFEADR
                     VTLVLDDADGQLALPGRGTHIRVMLGWKGEPLVDKGTYIVDEVAHEGPPDRLTLSANS
                     ADFREEFNVKREVSWHDVTVERVVSAIAHRYGLKAQISDMLMDIEIDHADQTEESDMS
                     FLTRMADMLGAITTVKNGCLLFILPGGGVTADGRPLPSFALARSDGDSHQFRIADRQA
                     YTGVKAYWLDLNHGKKKAVNIKKRKTHAEKSSSREGSYMEGAEGNVFVLRKTYPNEIA
                     AQRAAAARWQQLQRGAAEFSITLARGRAELYPEMHGTVTGFKTEIDNQDWLIAKVEHN
                     IDGSGFTTRLELEARIPEWIAEKESNG"
BASE COUNT         2406 a         2359 c         2550 g         2429 t
ORIGIN      
        1 tgattaatta ttatcgccag aacgaaatgt gcacgaaaaa ttgttataag aattgtgtca
       61 cttttatcat tgtgatatga ccacttcgtc attaatctca tgagcgttta aaatactacc
      121 gccaaatcac gcaacgaact ttgctggatt cagtttatgc tgctgtaaat ccgctcgcag
      181 taaacctttc agacgcaccg tgatgttatc agtggttctt taagcgtttt gctggtgtac
      241 tcactacaaa cgaattgcga atcgaatcaa tgtgaaacgg aaaggtacaa tctccctttt
      301 tttagtgtta acatcgctca accgggtgga aggtgagagg ttaatgtctt tcatttgtgt
      361 acctgattgc gaaccaattt agcatttgtt ggctagatgg tttcgcaatg aactgttaat
      421 aaacaaattt ttctttgtat gtgatctttc gtgtgggtca ccactgcaaa taaggatata
      481 aaatgcctgt tataactctt cctgatggca gccaacgcca ttacgaccac gctgtaagcc
      541 ccatggatgt tgcgctggac attggtccag gtctggcgaa agcctgtatc gcagggcgcg
      601 ttaatggcga actggttgat gcttgcgatc tgattgaaaa cgacgcacaa ctgtccatca
      661 ttaccgccaa agacgaagac ggtctggaga tcattcgtca ctcctgtgcg cacctgttag
      721 ggcacgcgat taaacaactt tggccgcata ccaaaatggc aatcggcccg gttattgaca
      781 acggttttta ttacgacgtt gatcttgacc gcacgttaac ccaggaagat gtcgaagcac
      841 tcgagaagcg gatgcatgag cttgctgaga aaaactacga cgtcattaag aagaaagtca
      901 gctggcacga agcgcgtgaa acattcgcca accgtgggga gagctacaaa gtctccattc
      961 ttgacgaaaa catcgcccat gatgacaagc caggtctgta cttccatgaa gaatatgtcg
     1021 atatgtgccg cggtccgcac gtaccgaaca tgcgtttctg ccatcatttc aaactaatga
     1081 aaacggcagg ggcttactgg cgtggcgaca gcaacaacaa aatgttgcaa cgtatttacg
     1141 gtacggcgtg ggcagacaaa aaagcgctta acgcttacct gcagcgcctg gaagaagccg
     1201 cgaaacgcga ccaccgtaaa atcggtaaac agctcgacct gtaccatatg caggaagaag
     1261 cgccgggtat ggtattctgg cacaacgacg gctggaccat cttccgtgaa ctggaagtgt
     1321 ttgttcgttc taaactgaaa gagtaccagt atcaggaagt taaaggtccg ttcatgatgg
     1381 accgtgtcct gtgggaaaaa accggtcact gggacaacta caaagatgca atgttcacca
     1441 cgtcttctga gaaccgtgaa tactgcatta agccgatgaa ctgcccgggt cacgtacaaa
     1501 ttttcaacca ggggctgaag tcttatcgcg atctgccgct gcgtatggcc gagtttggta
     1561 gctgccaccg taatgagccg tcaggttcgc tgcatggcct gatgcgcgtg cgtggattta
     1621 cccaggatga cgcgcatatc ttctgtactg aagaacaaat tcgcgatgaa gttaacggat
     1681 gtatccgttt agtctatgat atgtacagca cttttggctt cgagaagatc gtcgtcaaac
     1741 tctccactcg tcctgaaaaa cgtattggca gcgacgaaat gtgggatcgt gctgaggcgg
     1801 acctggcggt tgcgctggaa gaaaacaaca tcccgtttga atatcaactg ggtgaaggcg
     1861 ctttctacgg tccgaaaatt gaatttaccc tgtatgactg cctcgatcgt gcatggcagt
     1921 gcggtacagt acagctggac ttctctttgc cttctcgtct gagcgcttct tatgtaggcg
     1981 aagacaatga acgtaaagta ccggtaatga ttcaccgcgc aattctgggg tcgatggaac
     2041 gtttcatcgg tatcctgacc gaagaattcg ctggtttctt cccgacctgg cttgcgccag
     2101 ttcaggttgt tatcatgaat attaccgatt cacagtctga atacgttaac gaattgacgc
     2161 aaaaactatc aaatgcgggc attcgtgtta aagcagactt gagaaatgag aagattggct
     2221 ttaaaatccg cgagcacact ttgcgtcgcg tcccatatat gctggtctgt ggtgataaag
     2281 aggtggaatc aggcaaagtt gccgttcgca cccgccgtgg taaagacctg ggaagcatgg
     2341 acgtaaatga agtgatcgag aagctgcaac aagagattcg cagccgcagt cttaaacaat
     2401 tggaggaata aggtattaaa ggcggaaaac gagttcaaac ggcgcgccct aaccgtatca
     2461 atggcgaaat tcgcgcccag gaagttcgct taacaggtct ggaaggcgag cagcttggta
     2521 ttgtgagtct gagagaagct ctggagaaag cagaagaagc cggagtagac ttagtcgaga
     2581 tcagccctaa cgccgagccg ccggtttgtc gtataatgga ttacggcaaa ttcctctatg
     2641 aaaagagcaa gtcttctaag gaacagaaga aaaagcaaaa agttatccag gttaaggaaa
     2701 ttaaattccg tcctggtaca gatgaaggcg actatcaggt aaaactccgc agcctgattc
     2761 gctttctcga agagggtgat aaagccaaaa tcacgctgcg tttccgcggt cgtgagatgg
     2821 cgcaccagca aatcggtatg gaagtgctta atcgcgtgaa agacgatttg caagaactgg
     2881 cagtggtcga atccttccca acgaagatcg aaggccgcca gatgatcatg gtgctcgctc
     2941 ctaagaagaa acagtaaggc cttcaagtag cactgtctgt ggagccttcg ggcttcacag
     3001 gctttgttcg cctttgtttc gtttattaac aatgcgaagt ggaagttatt aaaatgccaa
     3061 aaattaagac cgtacgcggt gctgctaagc gcttcaaaaa aaccggtaaa ggtggtttta
     3121 agcacaagca cgctaacctg cgtcacattc tgaccaaaaa agcgaccaaa cgtaaacgtc
     3181 acctgcgtcc aaaagccatg gtttccaaag gcgatctggg cctggtaatc gcgtgcctgc
     3241 cgtacgcata agccgttaac gtttttaact ttttaattag aatatagata caggagagca
     3301 catatggctc gcgtaaaacg tggtgttatt gcacgcgcac gtcacaagaa aattttgaaa
     3361 caagctaaag gctactacgg tgcgcgttct cgcgtatacc gcgttgcctt ccaggctgtt
     3421 atcaaagctg gtcagtatgc ttaccgtgac cgtcgtcaac gtaagcgtca gttccgtcaa
     3481 ctgtggattg cgcgtatcaa cgcagcagca cgtcagaacg gtatttctta cagcaaattc
     3541 atcaatggcc tgaaaaaagc ctctgttgaa atcgaccgta agatcctggc tgatatcgca
     3601 gtattcgaca aagtagcgtt caccgctctg gttgaaaaag cgaaagcagc tctggcataa
     3661 gccagttgaa agagggagct agtctccctc ttttcgtttc aacgccatca aaacattgac
     3721 ttttatcgcc gtagcctttt caataaaggt cttttgaaga gtaccaaaag gtaacgcaag
     3781 caatgaatgc tgctattttc cgcttctttt tttactttag cacctgaatc caggaggcta
     3841 gcgcgtgaga agagaaacgg aaaacagcgc ctgaaagcct cccagtggag gctttttttg
     3901 tatgcgcgtt ttgaaaaata aaaagcctgt ctcatcaggt gtaattgtcg cagccagtct
     3961 ggacacggcc agcgcggaga aaccggagcg tatttaagta cgtgagaatt tcgagcactg
     4021 cccgggacca aaatggcaag taaaatagcc tgatgggata ggctctaagt ccaacgaacc
     4081 agtgtcacca ctgacacaat gaggaaaacc atgtcacatc tcgcagaact ggttgccagt
     4141 gcgaaggcgg ccattagcca ggcgtcagat gttgccgcgt tagataatgt gcgcgtcgaa
     4201 tatttgggta aaaaagggca cttaaccctt cagatgacga ccctgcgtga gctgccgcca
     4261 gaagagcgtc cggcagctgg tgcggttatc aacgaagcga aagagcaggt tcagcaggcg
     4321 ctgaatgcgc gtaaagcgga actggaaagc gctgcactga atgcgcgtct ggcggcggaa
     4381 acgattgatg tctccctgcc aggtcgtcgc attgaaaacg gcggtctgca tccggttacc
     4441 cgtaccatcg accgtatcga aagtttcttc ggtgagcttg gctttaccgt ggcaaccggg
     4501 ccggaaatcg aagacgatta tcataacttc gatgctctga acattcctgg tcaccacccg
     4561 gcgcgcgctg accacgacac tttctggttt gacactactc gcctgctgcg tacccagacc
     4621 tctggcgtac agatccgcac catgaaagcc cagcagccac cgattcgtat catcgcgcct
     4681 ggccgcgttt atcgtaacga ctacgaccag actcacacgc cgatgttcca tcagatggaa
     4741 ggtctgattg ttgataccaa catcagcttt accaacctga aaggcactct gcacgatttc
     4801 ctgcgtaact tctttgagga agatttgcag attcgcttcc gtccttccta cttcccgttc
     4861 accgagcctt ctgcagaagt tgacgtcatg ggtaaaaacg gtaaatggct ggaagtgctg
     4921 ggttgcggga tggtgcatcc gaacgtgctg cgtaacgttg gcatcgaccc ggaagtttac
     4981 tctggtttcg ccttcgggat ggggatggag cgtctgacta tgttgcgtta cggcgtcacc
     5041 gacctgcgtt cattcttcga aaacgatctg cgtttcctca aacagtttaa ataaggcagg
     5101 aatagattat gaaattcagt gaactgtggt tacgcgaatg ggtgaacccg gcgattgata
     5161 gcgatgcgct ggcgaatcaa atcactatgg cgggcctgga agttgacggt gtagaaccgg
     5221 ttgccggtag cttccacggc gtggtcgttg gtgaagtggt tgagtgtgcg cagcatccga
     5281 acgctgacaa actgcgtgtg acaaaagtga atgttggcgg cgatcgcctg ctggacatcg
     5341 tctgcggtgc gccaaactgc cgtcagggcc tgcgtgtggc ggtagctacc attggtgctg
     5401 ttctgccggg tgatttcaaa attaaagcgg cgaaactgcg tggtgaaccg tctgaaggga
     5461 tgctgtgctc cttttctgag ctgggaattt ctgacgatca tagcggcatt atcgagctgc
     5521 ctgcggatgc gccgattggc accgacatcc gtgaatacct gaaacttgat gacaacacca
     5581 tcgaaatcag cgtgacgcca aaccgtgccg actgtttagg tatcattggt gttgcgcgtg
     5641 acgttaccgt gctgaatcag ctgccgctgg ttgaaccgga aatcgttccg gttggtgcga
     5701 ccatcgacga cacgctgccg attacagtcg aagcgccgga agcctgcccg cgttatcttg
     5761 gccgtgtggt aaaaggcatt aacgttaaag cgccaactcc gctgtggatg aaagaaaaac
     5821 tgcgtcgttg cgggatccgt tctatcgatg cagttgttga cgtcaccaac tatgtactgc
     5881 tcgaactggg ccagccgatg cacgctttcg ataaagatcg cattgaaggc ggcattgtgg
     5941 tgcggatggc gaaagagggc gaaacgttgg tactgcttga tggcactgaa gcgaagctga
     6001 atgctgacac tctggtcatc gccgaccaca acaaggcgct ggcgatgggc ggcatctttg
     6061 gtggcgaaca ctctggcgtg aatgacgaaa cgcaaaacgt cttgctggaa tgcgctttct
     6121 tcagcccgct gtctatcacc ggtcgtgctc gtcgtcatgg cctgcatact gatgcgtctc
     6181 accgttatga gcgtggcgtt gatccggcac tgcagcacaa agcgatggaa cgtgcgaccc
     6241 gtctactgat cgacatctgc ggtggtgagg ctggtccggt aattgatatc accaacgaag
     6301 caacgctgcc gaagcgtgca accatcactt tacgtcgtag caaactggat cgcctgatcg
     6361 gccatcatat tgcggatgag caggtaactg acattctgcg tcgtctcggc tgcgaagtga
     6421 ccgaaggcaa agacgagtgg caggcagttg cgccgagctg gcgtttcgat atggagattg
     6481 aagaagatct ggtcgaagaa gtcgcgcgtg tttacggcta caacaacatc ccggatgagc
     6541 cggtacaggc aagcctgatt atgggtactc accgtgaagc tgacctgtcg ctcaagcgcg
     6601 tgaaaacgct gctcaacgac aaaggctatc aggaagtgat cacctacagc ttcgttgatc
     6661 cgaaagtgca gcagatgatc catccaggcg ttgaagcctt actgctgcca agcccgatct
     6721 ctgttgaaat gtcagcaatg cgtctttctc tgtggaccgg cctgctggca accgtggtgt
     6781 acaaccagaa ccgtcagcag aaccgtgtgc gcattttcga aagcggtctg cgtttcgtac
     6841 cagatactca ggcaccgttg ggcattcgtc aggatctgat gttagccggt gtgatttgcg
     6901 gtaaccgtta cgaagagcac tggaacctgg caaaagagac cgttgatttc tatgatttga
     6961 aaggcgatct tgaatccgtt ctcgacctga ccggtaaact gaatgaggtt gagttccgtg
     7021 cagaagcgaa tccggcactg catccggggc aatccgcagc gatttatctg aaaggtgaac
     7081 gtattggttt tgttggggtt gttcatcctg aactggaacg taaactggat cttaacggtc
     7141 gcactctggt gttcgaactg gagtggaaca agctcgcaga ccgcgtggtg cctcaggcgc
     7201 gcgagatttc tcgcttcccg gcgaaccgtc gtgacatcgc ggtggtggtc gcagaaaacg
     7261 ttcccgcagc ggatatttta tccgaatgta agaaagttgg cgtaaatcag gtagttggcg
     7321 taaacttatt tgacgtgtac cgcggtaagg gtgttgcgga ggggtataag agcctcgcca
     7381 taagcctgat cctgcaagat accagccgta cactcgaaga agaggagatt gccgctaccg
     7441 tcgccaaatg tgtagaggca ttaaaagagc gattccaggc atcattgagg gattgaacct
     7501 atggcgctta caaaagctga aatgtcagaa tatctgtttg ataagcttgg gcttagcaag
     7561 cgggatgcca aagaactggt tgaactgttt ttcgaagaga tccgtcgcgc tctggaaaac
     7621 ggcgaacagg tgaaactctc tggttttggt aacttcgatc tgcgtgataa gaatcaacgc
     7681 ccgggacgta acccgaaaac gggcgaggat attcccatta cagcacggcg cgtggtgacc
     7741 ttcagacccg ggcagaagtt aaaaagccgg gtcgaaaacg cttcgcccaa agacgagtaa
     7801 tctgatctaa ctaaaaaggc cgctctgcgg ccttttttct ttgtgtaatg tgttgatttt
     7861 tatggtttat ttttgttttg ctccactata tgtccacgga cttcgggtgg taaatacaaa
     7921 aaagcccgca gtttttacgc tgcgggcttt gtttttcttt gtgacttgcg tgtgagggtg
     7981 ctactttcgg ctttaccctg gcaaccgatt gacgggggat tactcccccg tcgcggtttc
     8041 cttactgttt acactgtagg aacgccgcaa actccgctcc ccatatgttc atccgtattt
     8101 cacacagcga accatgtaac atccagatga tgaggattgc cgtcacgcag aacgtgatgg
     8161 ccataagcga tttttgcgac atagcgcttg ctcctttgtt ggagaggcgc taacctatca
     8221 cttgcttatg gtagatggtt agggcctcgg gttaacgttt agttgactcg gggcctttcc
     8281 acatccggcc ttcaggtaat ccttccagcc gtcagccgaa aggcacccgc gcgtaatgta
     8341 ctggttttaa agccataccg caatcctcca gttgtagcgt taactgactg cgtccctgat
     8401 gggatttgcg cattgtctcc cgtggaatat ctttatcgtg gaagatcaac tgaggctggc
     8461 caataattct gtgcacactt tccattgttg aaaatgtggc gctgcactca aggttattgc
     8521 actgatagta tttgtagcgt atggtgccgg tatcattggc ggctttactg gtacggatcc
     8581 gggcagatgc gccgcagaat ggacaatgaa acattgatat acccccctgg ctgaactggg
     8641 gttgaactcg tcgccattct aaccattgct ttccttttcg gctatccact caggtattct
     8701 tgcctccagt tcaagccttg tggtgaagcc gcttccgtcg atattgtgtt ccacttttgc
     8761 aatgagccag tcctgattgt ctatctcggt tttaaagccg gttaccgtgc catgcatttc
     8821 agggtaaagc tccgctctgc cccgggccag cgtgatggag aattcagccg caccgcgctg
     8881 taattgctgc cacctggcgg cagctgcgcg ctgtgcagca atttcatttg ggtaggtttt
     8941 tctcagaacg aacacgttac cttctgcccc ctccatgtag ctgccttccc ggctgctgct
     9001 cttttcagcg tgagtttttc gtttttttat gttgacggct ttcttttttc catgattcaa
     9061 atccagccag tacgccttca cccctgtgta tgcctggcga tcggctattc tgaactggtg
     9121 actgtctccg tcactgcgtg ccagtgcaaa tgaggggagt gggcgcccgt cagctgtcac
     9181 gccgccaccc ggcagaatga acagcagaca gccatttttg acggtagtga tggctcccag
     9241 catatcagcc atgcgggtaa ggaatgacat gtcgctttct tccgtctggt cggcatgatc
     9301 gatttcaata tccataagca tatcgcttat ctgggctttc agtccgtaac ggtgggcaat
     9361 agcggaaacc acgcgttcaa cagtcacgtc gtgccaggat acttcccgtt tgacgttaaa
     9421 ttcctccctg aagtctgcgc tgtttgcact cagtgtcagc cgatccggag gtccttcatg
     9481 agcgacctca tcaacaatgt aggtgccttt atcaaccaga ggctctccct tccatcccag
     9541 catgactctg atgtgtgttc cccgcccggg gagagccagc tgcccgtcgg catcatccag
     9601 cacaagagtg accctgtctg cttcaaaccc ccggttgtct gtcatggaaa gcgatatcac
     9661 ccggttattc agttcctgaa gagtgttttt atcctgctcc ctgtccagtg aaacgctgaa
     9721 atcaggcatt ttcaccagtt cttt
//
LOCUS       DAZGVQ010000185         9618 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000185 DAZGVQ010000000
VERSION     DAZGVQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.186"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            297..713
                     /locus_tag="ACHLAG_004482"
     CDS             297..713
                     /locus_tag="ACHLAG_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657597.1"
                     /translation="MIKKTSETESVVRFVSVRGRATLYIPDEHLHHCDEKHIPILIVW
                     KRTVYADVTWLNDSLMLIHRDLFEREEFRRDIEERAEKIYEKYSANSKRSARAIAHHF
                     MTLYDLKAEDAEKAACDLFDMTMGIIQEYRNKERRP"
     gene            710..1159
                     /locus_tag="ACHLAG_004483"
     CDS             710..1159
                     /locus_tag="ACHLAG_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0657598.1"
                     /translation="MTAQIAAYGRLVDDQQVKQTSKGTPMALARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGEIRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALERAKRQTGQQGNQPPAGGDDPFSDDIPF"
     gene            1176..1592
                     /locus_tag="ACHLAG_004484"
     CDS             1176..1592
                     /locus_tag="ACHLAG_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFP0657599.1"
                     /translation="MNQQTTQQDQTTRRPTERPSWRHMTKRQRKNRRRINRLAEYWPE
                     LFGAETIKPLKTGIIRDVLEDAKSRELPIGHGVLRGALTSYIHTSRYLKAIVAGGPRY
                     GLNGQPCGEVTEEDKSVASELLKRRLEQMKQKNSQQ"
     gene            1612..1905
                     /locus_tag="ACHLAG_004485"
     CDS             1612..1905
                     /locus_tag="ACHLAG_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0657600.1"
                     /translation="MITDSVAQKLEERGLWRRAATRWSEVLLHAETDREREEAARRRA
                     ICIIKSRRMPEQFVTFGDVKKAADRTLKEMGINPQDEWRNYSFSDAGDDLALS"
     gene            complement(2352..2738)
                     /locus_tag="ACHLAG_004486"
     CDS             complement(2352..2738)
                     /locus_tag="ACHLAG_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657601.1"
                     /translation="MRLTQYKKEILSYFTPDNLAWVTAEIGSPPFDVSGVAYLLYGMD
                     SHNNRYQLESTRRTLEAMVRDGLLERVNVYERRDSVGHGVRRKVARYGLPGRSVVARD
                     GEGRNGAIDGECIRINATGVQLVDGQ"
     gene            2823..3125
                     /locus_tag="ACHLAG_004487"
     CDS             2823..3125
                     /locus_tag="ACHLAG_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0657602.1"
                     /translation="MIRDDKAEALENSGLWRRAAERWGELFRQAKTDEERHYITQRRA
                     QCTAKATRPKTTEWGHVCAVRAAATRTLKDMGIDQKKEDPLRGTKYRGETGRRDGS"
     gene            3127..3294
                     /locus_tag="ACHLAG_004488"
     CDS             3127..3294
                     /locus_tag="ACHLAG_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP0657603.1"
                     /translation="MDTITDKKAEQLESQGLWRRAAARWLDVMKEAHTDPQREHIARR
                     REICLANFRML"
     gene            3863..4024
                     /locus_tag="ACHLAG_004489"
     CDS             3863..4024
                     /locus_tag="ACHLAG_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657604.1"
                     /translation="MTDRSPTEVIHGIIEEQEERELILMIEEQRERQRKQESTQPDSV
                     SAENQADVP"
     gene            4678..5070
                     /locus_tag="ACHLAG_004490"
     CDS             4678..5070
                     /locus_tag="ACHLAG_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP0657605.1"
                     /translation="MRDIQQVLERWGAWAANNRENVTWSSVAAGFKGIIPAKITSRSQ
                     CCDDDAMVICGCIARLYRNNRDLHDLLVDYYVLGKTFMALAHKHECSDSLIGKRLQKA
                     EGVVEGMLMMLDVKLEMDRYVERDSVTA"
     gene            5360..5566
                     /locus_tag="ACHLAG_004491"
     CDS             5360..5566
                     /locus_tag="ACHLAG_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657606.1"
                     /translation="MEGVVSHEQAGHRASKNHRFVLFFSQGKNATGINPSSGGISRQH
                     DEQAAQPDTTSRRYYDFRNTFSKA"
     gene            complement(6282..6656)
                     /locus_tag="ACHLAG_004492"
     CDS             complement(6282..6656)
                     /locus_tag="ACHLAG_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657607.1"
                     /translation="MDTHDKIPPAYSYSPQPVPEAWKMRFSANSAWFFTDDFSVVNAW
                     LKAHSDGVEVVPLFCGSDSDGKRDTAFEEVCAALARMLHMHRLLLEKTRYQEHVTDPE
                     CLRELHEAPIQALNILVKHKLS"
     gene            7406..9016
                     /locus_tag="ACHLAG_004493"
     CDS             7406..9016
                     /locus_tag="ACHLAG_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFP0657608.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
     gene            9029..9148
                     /locus_tag="ACHLAG_004494"
                     /pseudo
     CDS             9029..9148
                     /locus_tag="ACHLAG_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            9108..9618
                     /locus_tag="ACHLAG_004495"
                     /pseudo
     CDS             9108..9618
                     /locus_tag="ACHLAG_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT         2896 a         2111 c         2386 g         2225 t
ORIGIN      
        1 ggcgacaatt gctcattttg aggcaacaat tgctcatttt gaggcaacaa ttacccattt
       61 tgaggcaaca cggctgacag gttttgtttg tctgaacgga acgcgttcaa cgtgatgttg
      121 cctcaaatac cccctattgt tgcctcaaat cgacatgatg ttgcctcaat ttttttgtga
      181 ttatcaatta aatcaatgcg ttaagacgtt tgaggcaact ggggcgactg aggcaacacg
      241 tttttcctat atatataaag cgtgtgaatg atgtgatgcc tggaaggaga cgattcatga
      301 tcaaaaaaac aagtgaaacg gaatctgttg tgaggttcgt atcggtacga ggcagagcca
      361 cgctttatat acctgacgaa catctgcatc actgcgatga aaaacatatc ccgatcctga
      421 ttgtctggaa gcgaacggta tacgccgatg tgacatggct gaatgattcg ctgatgttga
      481 tacaccgtga cctgtttgaa cgtgaagagt tccggcggga tattgaagag cgggcagaaa
      541 aaatctatga gaaatattca gcaaacagta aaagatccgc acgggcaatc gctcaccatt
      601 tcatgacgct gtatgacctg aaagcggaag atgcagaaaa agcggcatgt gatttattcg
      661 atatgacaat ggggattatc caggagtacc ggaacaagga gcgcagacca tgacagcaca
      721 gattgcagct tacgggcggc tggtggacga ccagcaggta aaacagacca gcaaaggaac
      781 accaatggca ctggcgcgta tggcggtatc tttgccatgc agtcaggcac aggacggaca
      841 ggcgacgtta tggctatcag tcctggcatt tggtaagcag gccgacgcgc tggcgagaca
      901 tgaaaaaggc gaccttatca gcgtggcggg taacatgcag atcagccagt ggaccggaca
      961 gaacggagaa atacggcagg gctggcaggt tatcgctgac agcgtaatca gtgcccgttc
     1021 ggcgcgtcct ggcgggaagc gggggcaaca ggggaaggcg accgaagcac tggaaagagc
     1081 aaaacgacag accggacagc agggaaatca gccaccagcg ggaggtgatg acccgttctc
     1141 tgacgatatt cctttctgaa tgacaggagc acaacatgaa ccagcagacc acacaacagg
     1201 accagaccac acgcagaccc acggagcgcc catcatggcg acacatgacg aagcggcagc
     1261 gaaaaaaccg caggaggata aaccgactgg ctgagtactg gcctgaactg tttggggcag
     1321 agactataaa gcccctgaaa acgggcataa tacgtgatgt gcttgaggac gctaaatcac
     1381 gggaattacc gattggtcat ggcgtattac gtggcgcatt aacctcatac atccacacgt
     1441 cgcgatacct gaaagccatt gtcgctggtg gccctcggta tggactgaac gggcagcctt
     1501 gcggtgaagt cacggaagaa gataaatcag tcgccagtga actactgaag cggagactgg
     1561 agcagatgaa gcagaaaaac agtcagcagt aatcagccgg aggtaaagcc agtgataacg
     1621 gacagcgtgg cacagaaact tgaggagcgc ggtttatggc gacgtgcggc gacccgatgg
     1681 tctgaggttc ttcttcacgc agaaacagac agggaacgcg aggaagctgc cagacgtcgg
     1741 gcgatatgta tcattaaatc ccgtcgtatg cctgagcagt ttgtcacctt tggcgatgtg
     1801 aaaaaggccg cagacagaac gctgaaagag atggggatta atccacagga tgaatggcga
     1861 aattacagct tcagtgatgc aggcgacgat ttagcgctat cgtgatacgc atctcatcgc
     1921 cgtatttgct caattatcgc gattcgctat aatcaccacc gaaaggccgt tgatggtgtt
     1981 cgcctcgggc aactggagcc cgactgaatg aagccagcca gcggcttttc ggcactcatt
     2041 ccggtgtatc tcccatacat cgggatgagt gaacgacgaa aacatcacgg tgatttcgtc
     2101 atgccgcgat aagaatgcgc ctctttgggc aattgaagcc ctctgtacag cgggttatcg
     2161 cggcaaagtt gtgataaaaa cgcgcctctt tgagcaactg gagctcactg taaagcggct
     2221 tatcacagca ggccgtaacg ggcgctcatc tttgggcaat tggagccctc tgtacagaga
     2281 cccgttgcgg ctgaccccga atgattttgc ccggggcaat tgaagcaccc cgcaaagtga
     2341 ttcggggttt tttattgccc gtccaccagc tgcacacctg tagcattgat gcgtatacat
     2401 tcgccgtcaa ttgcaccgtt ccggccctca ccgtcacgcg caacaacaga acgacctggc
     2461 aggccataac gcgccacctt tcgacgaacg ccatgaccaa cgctgtcacg ccgctcatac
     2521 acattaaccc gctcaagaag accatcacgc accattgctt caagcgtgcg ccttgttgat
     2581 tcgagctgat atcggttgtt gtgtgaatcc atgccataca gcaggtaagc cacaccggag
     2641 acatcaaacg gcggactgcc aatttcggca gtaacccacg ccagattgtc aggagtgaag
     2701 taactgagga tctctttttt gtattgcgtc agtctcatag tgacctttct tgttgtagga
     2761 tgaatccctc atacaataag ccataaggca aattttgaaa ggacagaatc ggcggggtac
     2821 agatgatacg tgatgacaag gctgaagcgc tggagaatag cggcctctgg cggcgtgctg
     2881 ctgaacgctg gggagaattg ttcagacagg caaaaactga tgaagagagg cactatatta
     2941 cccaacgaag ggcgcaatgc acggcaaaag ccaccagacc aaaaacaaca gagtgggggc
     3001 atgtatgcgc cgtcagggca gcagcgacca gaacgcttaa agatatgggg attgaccaga
     3061 aaaaagaaga tcctttacgg ggcacgaagt acaggggtga aacagggcga agagacggaa
     3121 gttaatatgg acacaataac tgataaaaaa gccgaacaac tggaatctca gggactatgg
     3181 cgaagagccg ccgctcgttg gcttgacgta atgaaagagg cccacaccga tccgcagcga
     3241 gagcatatag ccagacggcg ggaaatatgc cttgcgaact tcaggatgct gtaaataaga
     3301 ctgttgacga tatgggaatc agacgggatc ggcgacatta cgggttatgg aaataacagc
     3361 caggtaaatt ggtgaatata cggcaggctg caatcagagc cgaacatagc ggcctccgtc
     3421 accattatca gacagtagcg gtagtccttc catcacagca aaatgctacc cacttcacag
     3481 ccccttagaa gccccttcgg tggggtgaaa aatcccctga aaaatccctg aaaaaatgcg
     3541 caaaaaagtg cgcaaatcga tcataaaacc gatcacaaaa tcgatcacac agacaaacag
     3601 agcaaagcca gatatgacgc ggcttacagg gggatagatc acctcaaaaa gtgcgcaaaa
     3661 cgaaacacaa cagccgcgca aaacgaaacc gatacgtgat gatcgatttt tacgccagaa
     3721 atgaccagag caagggggct gatttccacc cttgtatttt gtgacttact taaagtaacc
     3781 cgcaaaaatg aacgttttta cagggcagaa acgagagtac tggttaacat tcctggcatc
     3841 accgcaactc acagggggac ctatgaccga ccgttcacca acagaggtaa ttcacgggat
     3901 tattgaagag caggaggagc gcgagttaat cctcatgatt gaagagcaga gagaacgcca
     3961 gcgcaaacag gagtccacgc agccggacag cgtgagcgct gaaaatcagg ctgatgtgcc
     4021 gtgatggtgt cgaactgagt aacttaaagt cactctgtga ccagttttaa acgagttgca
     4081 aaaagagccg gagaaaaacc acgaatctgg ggtttttact ggcgcggtgt acacggtgga
     4141 acgcatagcg ttacctgact tattgcattg taaaaagtta gaaaggcacg gcctgtaaga
     4201 tgggatgttg tgagtagttt aaagctaccc gcaaattctt tcgttgtgct ggcacaggcg
     4261 gttaaaccgt cggtaactgg ataatcccca ttgggggaaa gctggctaca cccctcacat
     4321 ctgaggactg ataacgcaaa attgcgttgg ctggcagagt gcaaatttgc acgatggcct
     4381 agcccattga ttctttcgaa aacccccatt gggggagaac gggggaataa acctgactca
     4441 ttgattcttc cacaatcctc aatttgagga agtcggcgcg gtaactcact gagattaaag
     4501 caaagctcaa atctgagatg gctacatccc tcacatctga ggactgttaa ccagcaagcc
     4561 ggaatatatt cacaaactac gaaattttcg tagtttaact ttcatccagt acatccccga
     4621 aaacacgggt atgtacggag ttaccgttac caataattat tcgcgaggtg attgttgatg
     4681 cgtgatattc agcaggttct ggaacgctgg ggagcgtggg cggcaaataa tcgcgagaat
     4741 gtgacctggt catccgttgc cgcaggattt aaggggatta ttccggcaaa gataacatca
     4801 cgctcacagt gctgtgacga tgatgcgatg gtgatatgcg ggtgcatagc ccgcctttac
     4861 cggaacaatc gcgatctgca tgacttgctg gttgattact acgtgctggg gaaaacgttt
     4921 atggcgctgg cacataaaca tgaatgctct gattcactca tcggaaagcg gctacagaaa
     4981 gcggaaggtg ttgttgaagg catgctgatg atgctggatg tgaagctgga gatggatcgg
     5041 tatgttgagc gcgattcagt gacggcataa cgcgcataaa aaaaccgcag ccatgaggaa
     5101 actgcggtgg agaaatacgg cttgccaatc aatcatgaaa aacgtaatcg cctgataatt
     5161 atgccacgag acaaaggacg gtgcaccctg aatggtcata atgaccactc agaccatcaa
     5221 ggcaaccata acgaacatcg ttacagttgg agcttttacc ccgttggggg aaaaggcgca
     5281 tacctgaatc atcattatgg tgatcaaggg tatgtaaagc ccaccagcct gattaaaggt
     5341 taaccggaaa aagccaggta tggagggagt ggttagccac gaacaagccg gacaccgcgc
     5401 cagcaaaaac cacagattcg tgcttttctt cagccagggt aagaacgcca cggggattaa
     5461 tccgtcgtct ggtggaatca gtcggcagca tgacgaacag gccgcgcagc cggacaccac
     5521 cagcagaagg tattatgatt ttcgtaatac ctttagcaaa gcgtgaatca tcattaccgt
     5581 agggatggtc ataatgacca caccttcccc caccggaaca aggaaggcag accaacgaag
     5641 atttcgtaag tcctgacccg cacaaggtat taaataatca ctacaagcca ttgattaaag
     5701 ttaattataa cgattcgtta gaatcaggct tttcagtctg gttaacggtt gaccggaaaa
     5761 aatctggtag ggtatccgcg atgttgggtt tattcatcgc ctgatgttgc ctcaaatccc
     5821 cctattgttg cctcaaatca gcatgatgtt gcctcaattt ttaagacgat attcaattaa
     5881 atcaatgcgt taattgattt gaggcaactg aggcgactga ggcgacacaa tttcgctata
     5941 tatataaagc gagtgagtga tctgaatggt catgatgacc acaccccacc ggaacaagga
     6001 cagaagaatt acgaaaattt cgtaaaatcc tccccgcatc ccccacggtt gcccgttaag
     6061 tgcaagcgca caggcacgac ctcgttaacc agttaccagc gctcacgctc aaatatccac
     6121 aggaatgggg ataaatcgta ttctggcgca atgttcactg tcggggcgag tattcaccgg
     6181 aaaacgtcat acaggcttac agtcggctta aaattagatg gacggatgac agggcgggat
     6241 ggtgagacct acaaaaaaaa gtgtaggtct ccatgacacg gtcaggacag tttgtgttta
     6301 accagaatat ttagcgcctg tatcggggct tcgtggagtt cccgcagaca ttcagggtca
     6361 gtaacatgtt cctggtatct tgttttctca agtaacaagc ggtgcatatg cagcatacgg
     6421 gcaagagccg cgcacacctc ctcaaatgcg gtatcccgct taccgtcact gtcagagcca
     6481 caaaacagcg gtacaacctc cacgccgtcg ctgtgggcct ttagccaggc attaaccacg
     6541 ctgaaatcat cggtgaagaa ccacgcactg ttagcgctga atcgcatttt ccaggcttcc
     6601 ggtactggtt gcggggagta tgaatatgct ggtggtatct tgtcgtgagt atccataggc
     6661 tatcgttccg tcattctgtt attcaccaca gcggtaaaaa cgccatgaag gaccagaata
     6721 ccgattgcct cgtttgctgt atgaggtgct taccacacat taggtacaca gatgaatcag
     6781 gtggaagtgt gattaccgga aaaaatccct ttcagtttga cgaaaaaaaa ccgcagtgtg
     6841 ataacactac ggcacgccac caaagaaaca taacaaggaa tcaacaatga gtaacacctg
     6901 taagccttcg gggtgattgt aacacatgag caatctttta ctggaaactg tttttcactg
     6961 gatcacgtat cgttatgggg attcccatat cggagagcat ccaccagctt cataggggat
     7021 aaccggaaaa attccggtaa atatttgcct gagtaagtca tcaagcgcct gctaaatgtg
     7081 tatagaaatg tgtataggat tgattttata acatgtggaa atggctttat tactggttgc
     7141 atggcttgtt gttctattcc cttcgccgct ccaatttatc ttcacctgat caatctgcca
     7201 tttttgctgt atctgatttt ctatattatc ttaattcatc gcttcagact tttcgcaatc
     7261 gccgtaatac ttccccattt tgttgtaaat aaaaagcgtt tacgcaatgc aatccttttg
     7321 taacaaccca ggtaaaacac cttattttca agttgttaaa taagtggtta tctacgcttt
     7381 taacaaacga ttgatggaat attttatgaa agggaaatct gcattaactc ttctgctcgc
     7441 tggtatattt tcgtgcggca catgccaggc gacaggggca gaagtgacga gtgaatctgt
     7501 atttaacatt cttaattcca cgggggcagc aacagataaa agttatttat cgttaaatcc
     7561 agataagtat cctaattatc gtcttttgat tcactcagcc aaattacaaa atgaaataaa
     7621 gagccactat accaaagacg aaattcaagg attgctaaca ttaacagaaa acacacgaaa
     7681 gttaacgttg acggagaaac cctggggaac gtttattctt gcttctactt tcgaagatga
     7741 caaaacagcg gcagaaaccc actatgatgc tgtctggctg agggatagtt tatggggcta
     7801 tatggcatta gtatcagatc agggaaatag tgtagccgca aaaaaggttc tgctcaccct
     7861 gtgggattat atgtctacgc ccgaccagat taagcggatg caggacgtca ttagcaaccc
     7921 caaacgactg gatggagttc ccgggcaaat gaatgttgtg catatccgat ttgacagcaa
     7981 ctcccccgtg atggcagatg tgcaggaaga aggcaagccg cagctgtgga accataaaca
     8041 aaatgatgcg ttaggtcttt atcttgatct ccttatacaa gcaatcgaca ctgatacaat
     8101 taacgctgaa gattggcaaa aaggtgaccg attaaaatcg gttgcgctgc tgattgctta
     8161 tctggataaa gccaatttct atgttatgga agattccgga gcatgggaag aagatgcccg
     8221 attaaacaca tcttccgttg cactggtgac ctctggactg gaacgtttgt cgaacttact
     8281 ctcgaagaaa gactctgttt ttgtctctga tttactacgc gaagctaaag ccaatgaact
     8341 ggatgaacct ctttcaacaa cgaggttgaa ccacctcata gataaaggtt atgaaagaat
     8401 aacgttacag cttgatttag ggggagaatc cccaggttat ctggaaaaag ataaacatta
     8461 cagagaagcc gatgcggctt tacttaatgt tatttatcct gcaaatcttg caaagataaa
     8521 caccagaaga aaagagcagg ttctaaaaat agttaaaaaa ctagcaggtc cgtatggtat
     8581 aaaaagatac gagaaggata attatcagtc ggcaaatttt tggttcaatg atattaaaac
     8641 cgataccgat caaaattcac atgcgaaaag agaaaagagc ttcattccat caacagaagc
     8701 agaatggttt tttgactcat ggtatgcaaa atcagcagca attgtttata aagaatcaag
     8761 aaaggaagag tatttgaatg actcagtgca atttatgaat agatcattgg cccaaataac
     8821 aggcgagaat atgattggtg caaatggcag gagtgtacct gaaatggcac ttcccgaaag
     8881 ctataactat atccacaagt cgggaacttt gcatgaagcg ccaagcccta tcattccgct
     8941 aaattggtcg aaagcttcaa tgactctcat gttgaaagag atgtccaacc ttatcaatga
     9001 tgagggaatt aaatagtttt cataatgagt tacagcacaa cgttgggtat tgactgagca
     9061 agatgaacga caaggacaac gacatcagcc ttatgaggag caagaaaatg aaagcaaatt
     9121 cgaaaacgat cagaaggatg aaagttaatg ttcggtctga ggaaagtaaa tcgaaacact
     9181 catacgtttg aaaccacttt tcaaaattgg aaagaaaatg gcgaagatgt tgcattatta
     9241 atgcccgaat ttagtgctaa atggttaccg atagcagaag agagtggcag ctggtctgga
     9301 tgggtattgc ttggagaaat atttccctta atttcatctg agctcgctgg gatggcttta
     9361 atgccagaaa cggaaaggtt aataggggaa tggctcagtt tatccagttc cccgttaaat
     9421 ctaaagcatc ctgaactaaa atataatcgt ttgcacgtag gtaaagtatt tgatggagta
     9481 ttgagccctg ctcaaccact aataagaata tgaacagggg aattgaatct ttggttagat
     9541 aaagtcacag tctgccaata cggaaacgct ccagtgttag acaaaaaatc attatattgg
     9601 tccattcatt ttgtaatt
//
LOCUS       DAZGVQ010000186         9579 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000186 DAZGVQ010000000
VERSION     DAZGVQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9579
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.187"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(64..822)
                     /gene="yhdZ"
                     /locus_tag="ACHLAG_004496"
     CDS             complement(64..822)
                     /gene="yhdZ"
                     /locus_tag="ACHLAG_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP0657609.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIAVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /gene="yhdY"
                     /locus_tag="ACHLAG_004497"
     CDS             complement(830..1933)
                     /gene="yhdY"
                     /locus_tag="ACHLAG_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP0657610.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALQIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPRLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..3124)
                     /gene="yhdX"
                     /locus_tag="ACHLAG_004498"
     CDS             complement(1943..3124)
                     /gene="yhdX"
                     /locus_tag="ACHLAG_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP0657611.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFTVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3192..4217)
                     /locus_tag="ACHLAG_004499"
     CDS             complement(3192..4217)
                     /locus_tag="ACHLAG_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0657612.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4648..4869)
                     /locus_tag="ACHLAG_004500"
     CDS             complement(4648..4869)
                     /locus_tag="ACHLAG_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657613.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5122..8226)
                     /gene="acrF"
                     /locus_tag="ACHLAG_004501"
     CDS             complement(5122..8226)
                     /gene="acrF"
                     /locus_tag="ACHLAG_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFP0657614.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPTSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNSDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSISVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8238..9395)
                     /gene="acrE"
                     /locus_tag="ACHLAG_004502"
     CDS             complement(8238..9395)
                     /gene="acrE"
                     /locus_tag="ACHLAG_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFP0657615.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2535 a         2483 c         2289 g         2272 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgcagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggctcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccaccagaaa tttgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc tttggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtagaac ggtcagatga gggaagagat tgaaatgctg
      541 aaagaccatt cccacttcct gccggacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct accgcgatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac ctgaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttattga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gctggctata gcgcgacatg ctgaaacaga
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggtgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caacccgata atgatcacca
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cctaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ctgcccttta ggcagtgcct gtaatccgcc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa cgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctcc agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgacaca agcccaatct
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagcccatac ataaactgac
     1681 caaatcgttc gtggatgaag acccaacagg caccggcttt tgtgcagtcg gcacgcgttg
     1741 agccaaccca gttagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgctgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat agcggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aatcactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaatcc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagatg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc atcccgccgc ggaagttaaa gcctcgtagg gctgggacat
     2401 cccagtgtag tgctgcgcca aaaagccatt gcgccagtaa aggcaaacca atgatcaaca
     2461 ctgcggcgat cggccaggtg cggcgcagtt gtccggtctt tatctggtat gttttattaa
     2521 agcggaatag cccaacagaa aggactatag ccataacaac agccaggata aacgcaataa
     2581 atccgtctcc cagctgcggt gacggaatat aaagcccacg attgctcaaa aaggccagat
     2641 caaacgcgct cacagcctgg cgtggtccgg gcaaattgcg caacacggta aagtaccaga
     2701 agaagatttg cagcagcggg ggaatattac ggaagatctc aatataaatt gtggatagtt
     2761 ttcgtagcag ccagttatcc gaaagtctcg ccagaccgat aaagaagcct aaaacagaag
     2821 cgaacacaat acacaatgca gaaaccagta gcgtattgag taagccgaca ataaaaacgc
     2881 gtccgtaggt gtcgccctgc tggtaatcga tcaaatgctg gacaataccg aagccagcgc
     2941 cgcgatccag aaaggcaaaa cctgaagtaa tgccacgatt attgagattc gttacagtgt
     3001 tgtgaaataa ccaaccaaca atgccgacaa cagcaacaac ggcaaggatc tggaataacc
     3061 aggcgcgaac cgtagggttg gcaaaagaga gtgagccttt aacggttgag cggcgatgag
     3121 acatagaaac ctcggaaagc ataacgctga agagagcgcc gccgtagcga cgcccgttac
     3181 ctcacaccga cttaacgcac gggcggcgcg tactgaatac cgccgttatt ccagagatta
     3241 ttttgcccac gtttaatttt cagcgggctt tctgaaccta cgttacgctc gaaaatttcc
     3301 gagtagttac ccacctgttt gatgatgtta taggcccatt tattatccag cttcagatcc
     3361 ttgccgtaat cgccttcttt acccagcaga tgtgccatat caggcgttgc tggattagcc
     3421 gctttttcat cgacgttctg ggaattgatg cccatctctt cagcattcag catggcgaaa
     3481 agcgtccagc gtacaatcga gaaccattca tcatcgccac gacgaactac cggcccaagc
     3541 ggttctttag agataacctc cggtaagaca atccattcag ctgggttgct taatttgatg
     3601 cgcagggcat acagttgcga ctgatccgag gccagcgtat cgcagcgacc agattccagt
     3661 gccttcgctg attcgtcaga gcgatcgaaa gtcaccggtg tgtacttcat attgtttgcc
     3721 ttgaaatagt cggcaacgtt gagttcggta tcagtacccg cctgaataca gacggtagcg
     3781 ccatccagtt ctttcgcgct ttttagcccc gctttatcgt gcgtcaggaa gccaatgccg
     3841 tcgtaataag tgacgccggt aaatgccatt cccatcccgg catcgcgaga tgaagtccag
     3901 gtcgtattac gggagagcaa atccacttcc cctgactgta aagcggtgaa gcgttctttt
     3961 gctgtgagtg gggtatattt cactttcgtg tcgtcaccaa atacagcagc ggcaacacca
     4021 cgacaaacat caacatcaat acctgaaaac ttaccgtctg catcggcata agagaaccca
     4081 ggtaatccat cactgatccc gcattgcaca aaaccttttt tctgaacggc atcaagcgtc
     4141 gcgccagcat gcgcctgatt tgcaacggca agcagcacgc tggcggcagc cagtgtggct
     4201 atcatcatct ttttcataat gcttcctgtg aggcgaaaat tatcgttata ggacgtttgg
     4261 gaaacgtctt ttacctgtct gtggccatcc tcttataagc aaaagtagtg ccagcttttg
     4321 cgcccgattt cgcgtcgcaa taagctgagt gtagacagga aataataaaa acagcgcccc
     4381 gcaatggtgc gaaaatgaaa catgcgccac attttggcgc aaaatattta cgcgtgtttt
     4441 actaagcaga ataatgattg ttagcgtgag tatatttctg gtgggaagtg tgaaagttaa
     4501 attgatgcgt tatttcgaaa gcagcgaaat aacgcgatat aacatgctaa atcagcagag
     4561 aattagccag cagaatatag caatatttta tcttatgtaa agatactgta tcgttaaaca
     4621 gagcgcgaaa ctccgtgccc tgttcagtta tttcgacaga gcgatgatcc ccatcgtcag
     4681 gccagcaaca gccgctgccc cgccagcaat agccccagcc gtttcccagt tatcctgagt
     4741 cgtgcctttc atacaggtat tggtgtgaac cagacgaccc tgatcgtcat aaaccggaac
     4801 gcagggcgaa tcgtgagcac agcccgccag caatgcggtg agcaatgcca caggaattaa
     4861 tcttttcatg gtgttacctc agtaataaac gctaacgctt aataagttaa ctctcttatt
     4921 aacgagctga atgccatttt atcaccggag gttataaatc ttgcggaatg aataatgaca
     4981 aattatgtga attgtaaaaa tagtgaaggg agcgggaact atagaaagct tttaaagagt
     5041 gagcagaaga atatcttatt aatttttatg ggtttcactg gaaataataa aggcacccga
     5101 aagcgccttt atgtttctga tttatccttt aaagcaacgg cggatcacca caaagaacac
     5161 cggtacgaag aagattgcca gcaacgttgc agagaccatt cctcccatta ccccgatacc
     5221 cacagcgttc tgcgcgccac tgccggcacc gttactgata gctagcggta atacgccgag
     5281 aataaaggcg agagaggtca tcaggatagg acgcagacgc atacgtactg ccatcagtgt
     5341 cgcttcaaca acacctttac cctctttctc catgagatct ttagcgaact caacaatcaa
     5401 aatagcgttt ttggccgaca agccaattgt cgttagcaag cccaccataa agtagacgtc
     5461 atttttttga ttaaagagtg ttgccgccag tagcacgccg acaatcccta acggcactac
     5521 caacataacc gagacagaaa ttgaccagct ttcatagagt gcagcaaggc acaggaaaac
     5581 aaccacaaag gaaattgcta ccagagcggg agcctggttt cccgataagc gttcctgata
     5641 cgacataccc gtccagtcat aaccaatgcc cgcaggtaat tttgacgcaa ggttttccat
     5701 caacgccatg gcatcgccgg aactggttcc tggcgcggct tccccctgaa tctccattga
     5761 cggcagaccg ttgtagcgtt ccagtcgcgg agagccatac acccaatgtg aagtggtaaa
     5821 ggccgagaat ggcaccattt cgccgttggc gctgcggaca taaagtttat cgacatcttc
     5881 tggcagcata cggaatttgg catccgcctg aacatacacc tttttcacgc gaccacggtc
     5941 gatgaagtcg ttaacgtaag tcccacccaa cgccgttgaa atggtctgat tgatgtcaga
     6001 aagtgaaaca cctaatgcct gcgccttttc ctggtcaact tccagtttaa actgcgcggt
     6061 gtcttccagg ccattaggac gcacgctgac taagctggca ggatgttgcg ccgccatgcc
     6121 aagcaactgg ttacgggcct gggtcagggc atcgtgaccc agcccagcct gatcaattaa
     6181 ctcaaagtcg aaacccgttg ccgtgcccag ttcaacaatg gctggcatat tgaatggaat
     6241 gacaaaaccg tcgcggatct tgcccaattc cattttggca cgatggatta ccgcttccgc
     6301 actgttttca tcactattac gctcttccca cggtttcaga ctgacgaagg ccataccggc
     6361 gttttgtgcc tggccgctga agctaaagcc gttaaccgta aagacacttt caacgttcgc
     6421 tttctcgttc ttcagatagt aatccgtcac ttgatccaac actttttgcg tccgctcttg
     6481 cgtcgcgcca gcgggtaact gaatcatggt cagaaagaca ccctgatcct cttcaggtaa
     6541 gaaggaagac ggaagacgta aaaacaacac caccattcct gcaacaatca gcgcatagat
     6601 cagtaaatat cgtcctgtgg aaccgaggat tttgccgacg ctgttggtgt agtggttaac
     6661 gctatgatcg aaggtggtat taaaccagcc gaagaaacca cccttatttt cgtgatgctc
     6721 agctgaggtg ggtttgagca gtgttgcaca taacgcaggg gtaagaatca atgccaccag
     6781 aacagaaagc gccattgccg aaacgatggt gatagagaac tggcgataaa ttgccccagt
     6841 agaaccgccg aagaatgcca tcggaataaa taccgctgac agcaccatcg cgatacccac
     6901 cagtgcgccc tgaatttgcg acatcgattt ttccgtcgct tcttttggcg ggagcttatc
     6961 ctccatcatc acgcgctcga cgttctccac caccactatc gcatcatcga cgagcagccc
     7021 gatggcaagc accatcccga acatcgttag tgtgttgatg gagtaaccaa aagcggcgag
     7081 gatggcaaac gtccctaata acaccacggg taccgcaatg gtggggatca gcgttgctcg
     7141 catattctgc aagaacagat acatcaccag gaacaccagc ataatggctt cgaacagcgt
     7201 ttttaccact tcgtgaatag aaagctggac gaatggcgtg gtgtcataag ggtagagaac
     7261 cttcattccc tgcgggaaga atggctgtaa ttccgccagt tttgccttaa tggctttcgc
     7321 ggtatcgaga gcattcgcgc cggttgccag cttaatcccc aggcccgccg ccggttttcc
     7381 gttgatacga gcgataacgt tatagttttc accgccaagt tcaactcgtg cgacatcttt
     7441 caggcgtacc accgagccgt cactgtttac gcgcagggtt actttgccga attcttccgg
     7501 attttttaac cgcgtctgag caataattga ggcgttcaac tgttgccctg gtaacgctgg
     7561 cgttccgccc aactgtccgg cagcgatctg atcgttctgt accttcaact ggttaatcac
     7621 atcaaccggt gtcagtttat atttgtttag cagatcggcg tccagccaga tacgcatcgc
     7681 atactgtgcg ccgaaaagct gtacgtcacc gacgccattc agacggctaa gcgtatcttt
     7741 aacgttagag gccacatagt ccgagatatc gtcctgtgtg gtgtctgggt tatcagagac
     7801 aaagcccgcc accatcaaat agctgctact ggacttttca acactgatcc cctgctgctg
     7861 aacctcctgc ggcagcaacg gcgtggcgag ctggagtttg ttctgcacct gcacttgcgc
     7921 gatatcagga tcggtcccgg actggaaggt aagggtaatt gtcacgctac cggcggaatc
     7981 gctggtggag gacatataca tcaggttatc gataccgttc atattctgtt cgataacctg
     8041 cgtcaccgta tcctgcacgg tctgcgcatc agcgcccgga tagtttgctg aaacagaaac
     8101 cgcaggcggt gcaatggttg gatactgagc gacgggtaat tgtaggatcg ccagtgcgcc
     8161 cgccatcatc agaataatgg ccagcaccca tgcaaatatc ggtcgtcgaa taaaaaagtt
     8221 tgccatgtca gattacctta cttcgatgca gtatctgcgg gggtatcggt agtggcttta
     8281 acctgctctc ccggacgcgc tttttgcagg ccgctgacaa tgacttgatc gccagatttc
     8341 agaccttcgc taatcaacca tttatcgcca atcgcctgac tggcaacgac agggcgcgct
     8401 tccacctgac ttttatcgtt aacaatcagc acggttgcat caccacgcgg tgtgcggcta
     8461 acgccttgtt gcgggataag aatggcgtca ggttggacgc cttcatcaat ccgtgcgcgc
     8521 acaaacatac ccggcaaaag cgtatgttgc gggttaggga agacggcacg tagggttatg
     8581 gagccggtgc tttcatcaac ggtcacatca gagaattgca gcgtaccttt caggggatag
     8641 gtttgaccgt tttccatgac caactctacg ttgctggtgg cgttttcctt atgcaaattt
     8701 ccttgctcta cggattgctt cagcctcata aaatcgttgc tggattgggt cacatcaacg
     8761 tagataggat cgagctgctg gacagtcgcc agttcagtcg tttgcccatt agtgacaaga
     8821 gcgccttcgg tcacggtcga tttgccgata cgtccgctaa ttggcgcagt cactttggta
     8881 taagcgagat tgatgcgagc gctttcgact gtggcttttg cggcaatcac ggcggcatcg
     8941 gcctggcgag catcagcaat ggcctggtcg tactcctgct ggctgatgta tttcgtaccc
     9001 acgagcggaa cgtaacgttt taccgtcaaa tgcgcgatgg cggcggcggc ttcacttttc
     9061 gccagttcgc ctttcgcgct gtcataattt gcctgatagg tcgcgggatc gatctggtac
     9121 agggactggc ctgcttgtac atcgctgcct tcagtgaaat tacgattcag tacgatcccg
     9181 ctaacctgtg ggcgaacttc ggctatacgg taagcattgg tgcggcctgg taattcagtc
     9241 ttaacttcta acggggccgt tttcacaata tgaacggtaa cctgcggttc accgacgtga
     9301 gctttctctt ctcccttatc gttacaaccg gcgattaaag ccgcggagat cagaataaag
     9361 gagggcagga ggaaaaacct ggcatgtttc gtcattacta ttcctcaaaa aaccaaaagc
     9421 gcgttattta cccagaaagg caacgcgtta actcgcagaa agaaaaatac agtttgctat
     9481 cctacaaatt atcattcgtc gatgtaagga atagttatga atactggcgt ctcaaggcac
     9541 ataaacacaa aaaaagatta atattctact gttttattt
//
LOCUS       DAZGVQ010000187         9566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000187 DAZGVQ010000000
VERSION     DAZGVQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.188"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(54..266)
                     /gene="cspA"
                     /locus_tag="ACHLAG_004503"
     CDS             complement(54..266)
                     /gene="cspA"
                     /locus_tag="ACHLAG_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFP0657616.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(547..837)
                     /locus_tag="ACHLAG_004504"
     CDS             complement(547..837)
                     /locus_tag="ACHLAG_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFP0657617.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1271..1981
                     /locus_tag="ACHLAG_004505"
     CDS             1271..1981
                     /locus_tag="ACHLAG_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFP0657618.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2031..3005)
                     /gene="ghrB"
                     /locus_tag="ACHLAG_004506"
     CDS             complement(2031..3005)
                     /gene="ghrB"
                     /locus_tag="ACHLAG_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFP0657619.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3109..3768)
                     /locus_tag="ACHLAG_004507"
     CDS             complement(3109..3768)
                     /locus_tag="ACHLAG_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFP0657620.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3921..6254
                     /gene="bisC"
                     /locus_tag="ACHLAG_004508"
     CDS             3921..6254
                     /gene="bisC"
                     /locus_tag="ACHLAG_004508"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFP0657621.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQERGIQD
                     GDTVRLWNARGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6223..6663)
                     /locus_tag="ACHLAG_004509"
     CDS             complement(6223..6663)
                     /locus_tag="ACHLAG_004509"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP0657622.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAINFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6660..7223)
                     /gene="tag"
                     /locus_tag="ACHLAG_004510"
     CDS             complement(6660..7223)
                     /gene="tag"
                     /locus_tag="ACHLAG_004510"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFP0657623.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFVDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7381..8079
                     /locus_tag="ACHLAG_004511"
     CDS             7381..8079
                     /locus_tag="ACHLAG_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP0657624.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            8308..9510
                     /locus_tag="ACHLAG_004512"
     CDS             8308..9510
                     /locus_tag="ACHLAG_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0657625.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         2244 a         2349 c         2498 g         2475 t
ORIGIN      
        1 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
       61 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      121 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacacg
      181 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      241 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      301 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      361 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      421 accgttgatg tgcattaagc cacgcattgg cgggtgatgc aacaattatt tttcatattt
      481 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      541 aaaagtctat tccatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      601 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
      661 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcgtaactg
      721 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
      781 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
      841 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
      901 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
      961 agtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttac
     1021 cactcctggt ttgtaaagat ttcgaaaatc taaccttctg cgaaggcgta aatctctggc
     1081 tacaaaaaaa gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1141 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1201 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1261 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1321 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1381 tatcgacttc ctgcagaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1441 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1501 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1561 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaacggttc
     1621 gctaggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     1681 tgcgctaaaa cagagcgatg acctgaaacc ggtctttgat caggcattca ccaaagtggt
     1741 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     1801 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     1861 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     1921 accggcacag catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     1981 aagagtaaaa gcccgagcga ttatcgggct ttactctacg cagtcgcggc ttagtccgcg
     2041 acgtgcggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2101 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2161 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2221 acatccagcc cggcggcgtg aatttcccct ttctgcaatg ctgcgatcag tgcattttcg
     2281 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2341 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2401 tctgactctt gtaacagtgt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2461 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcgcacgt
     2521 tgtgccagcg ccataccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2581 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     2641 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     2701 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     2761 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     2821 tccagcaatg cggcatcaac attctcgttt gaacccagta aaccttcagc ttcggcaaaa
     2881 attgctgcat tttgttcgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     2941 tgctcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3001 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3061 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3121 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3181 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3241 caacgcgctg gcaacggaat ccgcacgttg ctgggagaga cgcatgttca ggtcgtgacc
     3301 acccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3361 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc agggtcgcgc tgctgctgtc
     3421 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3481 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3541 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3601 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     3661 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     3721 cagagcgcca ctcactacgg cggcaataag ataaacacgt ttcttcattg ttaatcctta
     3781 ataacctttt tattctttgc cacgggttcc gtggcgggag attatgccgc gtgaacatga
     3841 agattattcc tgggaatact cggaaatttg taagtaatat ttaactgctc aatacatcta
     3901 acctttcagg agtcttcggt ttggccaact catcctcacg atattccgtt ctgactgccg
     3961 cccactgggg gcccatgctg gttgaaaccg acggcgaaac cgtgtttagc tcgcgtggcg
     4021 cgttagccac aggaatggaa aactccttgc agagcgcggt tcgcgaccag gttcacagca
     4081 atacgcgggt acgatttcca atggtgcgca aaggctttct cgcgtcaccg gaaaacccgc
     4141 aaggcattcg tgggcaggat gaatttgttc gcgtgagttg ggatgaggcg ctggatctta
     4201 ttcaccaaca acataaacgc attcgtgagg cttatggtcc ggcatcgatt tttgctggtt
     4261 cctacggctg gcgttcaaac ggcgtgctgc ataaggcctc gacattatta caacgctata
     4321 tggcgctggc aggcggttat accgggcatc tgggggatta ttcgaccggc gcggcacagg
     4381 cgatcatgcc gtatgtcgtg ggtggtagcg aagtttatca acagcagacc agttggccgc
     4441 tggtgctgga acatagcgat gtcgtggtgc tgtggagtgc taatccactc aatacgctga
     4501 aaattgcgtg gaatgcatcc gatgagcagg ggctttctta cttttctgca ctgcgtgaca
     4561 gtgggaaaaa gctgatctgc attgatccaa tgcgatcgga aaccgtcgat ttctttggcg
     4621 ataaaatgga gtgggtggca ccgcacatgg gcaccgatgt tgcgctgatg ctggggatcg
     4681 cccatacgct ggtggaaaat ggttggcacg acgaagcgtt tctggcgcgt tgcaccacag
     4741 gttatgccgt cttcgcctct tatttgctgg gcgagagtga cggaatagcg aaaaccgccg
     4801 aatgggcagc agagatttgt ggtgttggcg cagcgaaaat ccgcgagctg gcggctattt
     4861 tccaccaaaa taccaccatg ctgatggcag gctggggaat gcagcgccaa cagtttggtg
     4921 agcaaaaaca ctggatgatc gtcacgctgg cagcaatgtt ggggcaaatc ggcacacccg
     4981 gcggcggttt tggtctttct taccattttg ccaatggtgg taaccccacg cggcgttctg
     5041 cggtgctctc ttccatgcag ggcagcttgc cgggtggctg cgatgcggta gataaaatcc
     5101 ctgtggcccg cattgttgaa gcactggaaa accctggtgg cgcatatcaa cacaacggta
     5161 tggaccgaca ttttccggat attcgtttta tctggtgggc gggcggtgct aactttactc
     5221 atcatcagga taccaatcgt ctgatccgtg cctggcaaaa accggagctg gtggtgatct
     5281 ctgaatgctt ctggacggcg gcggcgaaac acgcggatat cgttctgcct gcgactacct
     5341 cttttgagcg taatgatctc accatgaccg gtgattacag taatcagcat ctggtgccga
     5401 tgaagcaagt ggtgccgcca cgctatgaag cgcgtaatga ttttgatgtt tttgccgagt
     5461 tgagtgaacg ttgggagaag ggcggctatg cgcggtttac ggaaggaaaa agtcagctgc
     5521 aatggctgga aacgttttat aacgttgccc ggcagcgcgg ggcaagccag caggttgaat
     5581 tgccgccatt tgctgagttc tggcaagcca accagttaat tgagatgccg gaaaacccgg
     5641 acagcgagcg gtttattcgc ttcgctgatt tttgccgcga tccgctggcg catccgttaa
     5701 aaaccgccag cggcaagatt gaaatcttct cacagcgtat tgccgattac ggttacccgg
     5761 attgccctgg gcatccaatg tggctggagc cggacgaatg gcagggcaat gccgaaccag
     5821 aacagttgca ggtactttct gcccatccgg cgcaccgcct gcacagccag ctgaattaca
     5881 gttctctgcg cgaattgtac gcggtggcaa atcgtgagcc tgtcaccatt catcctgacg
     5941 atgcccagga gcgcggcata caagatggcg atactgttcg gttgtggaac gcacgcgggc
     6001 aaattcttgc cggagcggtc attagcgagg gaattaaacc tggcgtgatt tgcattcacg
     6061 aaggggcatg gccggatctg gatttaaccg ctgacggtat ttgtaaaaac ggcgcagtga
     6121 acgtgctgac caaagatctc cccagctcgc ggctggggaa tggctgtgcg ggtaatacgg
     6181 cgctggcatg gctggaaaaa tacaacggtc cggaactgac acttacagcg tttgaaccac
     6241 cggccagctc ataatccatg tgggtagttg ggtttcatcc tgccatgcgc aatcgacaat
     6301 gtgaaaaccc tgtgcctggt aaaaatttat cgccggttga tttttttgat aaacctccag
     6361 catcaggtgg ggatggcgct gctgcacata ctgcatcagc gccttaccaa taccgcgcct
     6421 gacggccttc ggtgcgacaa acatcgctgc cagaaatcgg ccttccataa tgctgacaaa
     6481 accgagaagc ttaccgtctt cttcccagac ccagttttgc gcgttggcaa gataggcatc
     6541 ccgcaccagc ggaatgcagt cacgccagta attcgctttt ataaagggat gcccccaggt
     6601 tgtactttcc agccacagtt cgaggatcgc ggggagttct gaacgttgcg cttcccgaat
     6661 catggtttat ttcccggata gcaacagcag ccaaccacat gatcattcac cagcccacat
     6721 gcctgcataa aggagtaaca gattgtggtg ccgacaaact taaaaccacg ttttttcagt
     6781 gccttagata gggcgtcgga ggcggacgta gatgtgggaa tttcgctcaa cgttgtggct
     6841 tgtgtcacct gtggctgatg atttacaaac gaccagacaa agtcgacaaa cggttcgccg
     6901 ttctgttcca tttgcaggta cgcccgcgca ttaccaataa ttgcctgaat tttccctcga
     6961 tggcggataa tcccggcgtc ctgtaccagt ctttcgacat cctcttcctg cattgctgcg
     7021 accttcaccg gatcgaactg atgaaagcag gcgcgatagt tttcgcgttt tttgaggacg
     7081 gtgatccacg ataacccagc ctgctgccct tcaaggcaga tcatttcgaa cagtttttta
     7141 ctgtcagttt caggcacgcc ccactcatta tcatggtagg caatataaag cgggtcctga
     7201 ctcacccagc cgcaacgttc catactttcc ctcgctatgt acccgatcgt aaaaatttca
     7261 gccgcttttc ttgacgcatc ggtgaaaaag tcaatattta atacagggct actatgcccg
     7321 atatccttca tacaatgaca ataatattgc cgaattcggc tcttccctgg agtcgtgtta
     7381 atgatcataa aaaaaagcgg tggtcgctgg cagctaagcc tgctggcgag cgtggtaatc
     7441 agtgcctttt ttctcaacac agcttacgcc tggcaacaag aatatatcgt tgatacgcaa
     7501 cccggacatt ccacagagcg ttacacctgg gatagtgatc atcaacctga ttacaacgat
     7561 attttgtcgc aacgtattca aagtagccaa agggcgctgg gactggaagt caatctggcg
     7621 gaagaaactc ctgtggatgt gaccagcagt atgagtatgg gctggaattt tcctttatat
     7681 gaacaggtta caaccggccc ggtcgcggca ttacattacg atggcacaac cacctcgatg
     7741 tataacgagt ttggcgacag tactaccacg ctgaccgatc cgttatggca tgccagcgtg
     7801 agtagcttag gctggcgtgt tgactcccgg cttggcgatc tccgaccctg ggcgcaaatc
     7861 agctataacc agcaatttgg cgagaatatc tggaaggcgc aatcaggcct gagccggatg
     7921 acggcgacaa accagaacgg caactggctg gatgtcaccg taggcgctga tatgttgctc
     7981 aatcaaaata ttgccgccta tgccgcgcta tctcaggcag aaaataccac taacaatagc
     8041 gactatctgt atacgatggg ggttagtgcc agattttaac gtaaaaatca caattgaaac
     8101 cattggataa caacagttgt ggcgatagtg ggtgctaact tactaaataa taaatttggt
     8161 gaataattgt cgcgtcattc attcctgaac taaggcattt cattccgttc tgatggcatt
     8221 tcatgccgtt tttccccagg cataaaatgc acttcgttat ggttgtcggc agagattttt
     8281 cctttttatt actgcaggaa tactgccatg acaccttcaa attatcagcg tacccgctgg
     8341 ctgacactca tcggtactat cattacccag tttgcactgg ggtcggttta tacctggagc
     8401 ctgtttaatg gcgcgctttc tgccaagctg gatgcgccgg taagccaggt cgctttctct
     8461 ttcggcttgt taagtctggg gctggcaatt tcgtcttctg ttgcgggcaa attgcaggaa
     8521 cgttttggcg ttaaacgcgt caccatggct tccggcattt tgctgggatt aggcttcttc
     8581 ctgaccgcgc attccaacaa cctgatgatg ctgtggttaa gcgccggtgt gctggtcggt
     8641 ctggcggatg gcgcgggtta cctgctgacg ctctctaact gcgtgaagtg gttcccggag
     8701 cgtaaaggtc tgatctccgc gttcgctatc ggttcttatg gtctgggtag cctgggtttc
     8761 aaatttatcg acacgcagct gctcgaaacg gtgggtttgg aaaaaacatt tgtgatttgg
     8821 ggagcgattg cgctggtgat gattgttttc ggcgcaacgt taatgaaaga cgcaccaaaa
     8881 caggaagtga aaaccagcaa tggtgtggtg gagaaagatt acacgctggc agagtcgatg
     8941 cgtaaaccgc agtactggat gttagcggta atgttcctga ctgcctgcat gagcggcctg
     9001 tacgtgattg gggtagcgaa agatatcgcc caaagtctgg cacaccttga tgtggtttcc
     9061 gcagccaatg cagtaacggt tatttccatc gctaaccttt caggtcgtct ggtgctgggg
     9121 atactgtctg acaaaattgc ccgtatccgt gttatcacca ttggtcaggt gatttcgctg
     9181 gtgggtatgg cggccctgct gtttgcacca ttaaatgcag tgacgttctt tgcagcgatt
     9241 gcctgcgtgg catttaactt tggcggcact attaccgtct ttccgtcact ggtcagtgag
     9301 ttcttcggcc tcaataacct ggcgaaaaac tacggtgtga tttacctcgg tttcggtatc
     9361 ggtagcattt gtggttcgat tatcgcctca ctgtttggcg gcttctatgt gaccttctac
     9421 gtgattttcg ccctgctgat tctgtcattg gcgctttcta cgacaattcg tcagccagag
     9481 cagaaagtat tgcgtgaggc gcatatttag aaatatgttg ctactgaaat aaatgcgagg
     9541 agggagactt caaagtctcc cttttt
//
LOCUS       DAZGVQ010000188         9543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000188 DAZGVQ010000000
VERSION     DAZGVQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.189"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..275)
                     /locus_tag="ACHLAG_004513"
     CDS             complement(<1..275)
                     /locus_tag="ACHLAG_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657626.1"
                     /translation="MKVVFNVMDGFDKTFLPLEFSGFHGRNGYCYLRVQIKHGFIVFS
                     CAQLLNYYRTSVTNAIEQVREAAVNALFREGVLSYTQQKEFLDILKTS"
     gene            complement(440..1126)
                     /locus_tag="ACHLAG_004514"
     CDS             complement(440..1126)
                     /locus_tag="ACHLAG_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFP0657627.1"
                     /translation="MTTFIDHQKAFILLFNQTARYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVINMAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWGVACMMAQMQLGNVKALFENKPFITLSEPACGAGSMILAMADTLNRSGYPAY
                     RRMWVSATDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRILLTPGHYLGNWSYR
                     LRHVQEQAAA"
     gene            complement(1249..2196)
                     /locus_tag="ACHLAG_004515"
     CDS             complement(1249..2196)
                     /locus_tag="ACHLAG_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFP0657628.1"
                     /translation="MAEWCRNRFEITGKSVCLDVLTQWIEGCETPRYRHAIQQSIQLF
                     LIGCAGLVKPTRTTQYPPYPALVRHGTGMSSPANQAFEQWLGLLVKDAVLDEETIKTI
                     DRLYHQSCIGAVRWGNLPDNAREIITTLMHCQYSDWFGLVGLSEHIDAEACWSRLSDY
                     PEQAQPCDMLMVIPPRLATELNGSGGLLQGISTTTSLYSRIYGVEWPSGHNVRWVRDE
                     MSSLVLLTDTPWYPPSGELVGEISKVFDCEIRHWYSEPVRGIQGYNCYDGGEHTDSDP
                     QAEWPGREKLPQPRLYLVEERAEEQTEAAPLPVPLASGQ"
     gene            complement(2304..2738)
                     /locus_tag="ACHLAG_004516"
     CDS             complement(2304..2738)
                     /locus_tag="ACHLAG_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD29 domain-containing protein"
                     /protein_id="HFP0657629.1"
                     /translation="MNVKSVTEVDDAVVARVSDVLEFAFPGQKFNVLKVCDSGVYNMI
                     NVSWLDGPTEAEVRFITRAFEGKNGLRFVHESRKFSNEFVQECIDRLRKKYGQSNVPP
                     DVTVARYWKNDLWKIKTDRFPGNIDVAINEMGTETSKYRKVV"
     gene            complement(2815..3153)
                     /locus_tag="ACHLAG_004517"
     CDS             complement(2815..3153)
                     /locus_tag="ACHLAG_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657630.1"
                     /translation="MRPDLNILPGDSGCSVWFYNGMSQPRLLAGSIAGLLTDVTITSN
                     YRGDVTSEIHDVVQEWLATGRGNLADLKEELWYYNLYINPSADELMNASRRYGLGHTT
                     WLKDFISNAV"
     gene            complement(3221..3580)
                     /locus_tag="ACHLAG_004518"
     CDS             complement(3221..3580)
                     /locus_tag="ACHLAG_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657631.1"
                     /translation="MYSRFITGYWHLQSQSALLAHLCQLEGELAILRAWQRLGITPVP
                     EDEDEPSPLYSILWDVGEALGWLLYDLEMENAPAPGTIFHEVLDRVMSLGHETEHSIW
                     ADSISTGKRYAAIPDAF"
     gene            complement(3691..4674)
                     /locus_tag="ACHLAG_004519"
     CDS             complement(3691..4674)
                     /locus_tag="ACHLAG_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArdC family protein"
                     /protein_id="HFP0657632.1"
                     /translation="MKTKKHTSCPVNKTPERDLYQLVTDRVIEAIEKGVAPWQRPWRS
                     VRTHSGDPMMPVNALTRSAYRGINVLLLWLAAEEAGYSSNCWLTYRQAQALGGQVRKG
                     EKSTMAVIYKSWHKQDEDENGNRIFDAEGNPVMVDVPVLKANPLFNVEQCDGLSLPEV
                     TTTVTENGANDTLNAVADRVLTMVNACGVKIHSLPQDRAYYTPVFDRIVLPLSSQFTS
                     AEDYWSTLLHELVHSTGHAKRLNREGIVSSGGRYGDECYAFEELIAEMGSAFLCAQLG
                     VTGNVQHENYIGAWLKILRADKKALFSACRQAREATEYLLSVHDTPAEKAA"
     gene            complement(4767..4904)
                     /locus_tag="ACHLAG_004520"
     CDS             complement(4767..4904)
                     /locus_tag="ACHLAG_004520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657633.1"
                     /translation="MTFPTQTERFFFYLLYRVPASVAWVSDTLKTFDFERHSLVMLAL
                     W"
     gene            complement(4882..5361)
                     /locus_tag="ACHLAG_004521"
     CDS             complement(4882..5361)
                     /locus_tag="ACHLAG_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657634.1"
                     /translation="MNSLLILLYVVVCRACTILRVIAPVSSVRAWCEYQLEGMTILRD
                     AYSSLAKADGMQNDAFEYLESIARHLGYESGGDQLKSGLFRPFLWHIRAKGHRLGAVM
                     EMAVWQAWKNGCNDVCNPWGDVYNPWDRGWNDGWTECLDQRIAGLLKAVCIDFSDAD"
     gene            complement(5462..5842)
                     /locus_tag="ACHLAG_004522"
     CDS             complement(5462..5842)
                     /locus_tag="ACHLAG_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657635.1"
                     /translation="MGLDIFFLGRDRVTDAVVSVPETREVGYFRKVNPLIAWFETHCG
                     PVENAVERPVSRTELEALLSDLECLTPENCREFFPTTEGFFFGSQEYDQYYWKDVEIV
                     KSWVRSTLDSFDFERKILLLWAWW"
     gene            complement(6007..6408)
                     /locus_tag="ACHLAG_004523"
     CDS             complement(6007..6408)
                     /locus_tag="ACHLAG_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657636.1"
                     /translation="MFIPLLLRADMRITPFFHLLRQCLFNQKRCVHPQQPWSAPQLRH
                     LYCTPWFINATGNLSVRACIVSTNGALRHCFEFREGSHSREYRYWFEALPLSVVRFNV
                     FTGRISARRTLSAASRMAAQLTSFRYRFGLR"
     gene            6940..7671
                     /locus_tag="ACHLAG_004524"
     CDS             6940..7671
                     /locus_tag="ACHLAG_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger-containing protein"
                     /protein_id="HFP0657637.1"
                     /translation="MKSKYKKLKDELLRIAKACAPTPEDMLVYTGRARRLASFLKDAN
                     IQISSANSIKLRHIECYFQQRYRTGVSSKILREELDTIKHVLTHCGKRNIVKNERLTY
                     TSLNIADVRPIVICPYCGNKTNLIKGALMPFSISAATENKYYWICPPCNAWVGCHKNS
                     GRPLGTPAKENLRILRTKVRKLFDNYQQRANISRNEANIWLSRKLNCHIHECHIGYFD
                     EDMCNRASEIIITEINKNTYPPDSF"
     gene            complement(7675..8592)
                     /locus_tag="ACHLAG_004525"
     CDS             complement(7675..8592)
                     /locus_tag="ACHLAG_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFP0657638.1"
                     /translation="MARKSSRSKVELFRKSFISLARECGGSYATTADRMRIAKYFLNY
                     LRENGIKLRQTSSIKTRHISGYLLSRKAAGISHRTIQNERAVMRAILEKDGRLKIADP
                     SNPLLSNEALGLKDTCRDGKKIAVSPDEFWKAFAKVEKKNPGVAAAMQLSYVLGLRTK
                     EAVQSCKSVQSWLRELNSGCDSLSVVFGTKGGRPRNTTIIDRDAVRHALLYAQKIMDE
                     HDGKLIDRPNIKQAINVFRYHARKAGLSGVKSPHSMRYHFSQEARKFYRKNGYGESEI
                     YARVSMDLGHGDGRGRYVKQVYFNGSDES"
     gene            9335..>9543
                     /locus_tag="ACHLAG_004526"
     CDS             9335..>9543
                     /locus_tag="ACHLAG_004526"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657639.1"
                     /translation="MPSSSRKTVVTSALKGVQICPYIRDVSLQYSQLLSGYGGVKLTT
                     RRRSSQVIWSIGGRRSDMGHPKAFLT"
BASE COUNT         2626 a         2547 c         2094 g         2276 t
ORIGIN      
        1 gatgttttta atatatccag aaattctttt tgctgcgtgt atgacaatac cccctccctg
       61 aagagagcat taaccgcagc ctctctgacc tgctcaattg catttgttac tgacgtacga
      121 taatagttca ataattgagc gcatgagaaa acaataaatc catgtttaat ctgaaccctt
      181 aaataacagt acccattccg gccatgaaaa ccagaaaact ccaggggcaa aaaagtctta
      241 tcaaaaccat ccattacatt aaacacaacc ttcattgaaa taaaccccag tttatttttt
      301 tattggcact tgcaatcctt gagtttaaaa aacaagagta acctgtcaac accacttaca
      361 taaccataaa aaaaataaca aaaattgacc cacagatgta ataacgccag tcctgagact
      421 ggcgtagtga taaaaatgat caggccgctg cctgttcctg aacatggcgc agacgatatg
      481 accagtttcc gaggtaatgc ccgggtgtca gcagaatccg gcgccgctca ttacacagcg
      541 agttaccaat caccacttct ccggccacac cacacagcga caattgtata tacgccatac
      601 cagctgccag cgggtcaatg tccgttgccg acacccacat cctgcggtat gccggatacc
      661 ccgaacggtt cagggtgtca gccatagcca gaatcatact gccggcacca caggcaggtt
      721 cactgagcgt gataaagggt ttgttctcaa acaacgcttt cacattcccc agctgcatct
      781 gtgccatcat gcaggccaca ccccatggcg tgaaaaactg gctccggtat ttatccccca
      841 gctccagctg catgaacacg ctgcccagaa agtcgcagaa tccctgctcc agtccctcaa
      901 tgacatgggc aagcaaatgc gccatgttga taacatcctg cttttcataa ccactgacga
      961 ttcgcaagta cttctgctcc cgtgcttcac tgaactgaag ccggttctcc agggcaatca
     1021 cactgcagct gacaaagtcc tcaaacacct tatgacgttt gtgataacgg gcagtctgat
     1081 taaacagcaa aataaaagct ttctgatggt cgataaaggt ggtcatgtgc ttctccataa
     1141 aaaaacaggg aacacatggc cccggggagg aacaagtgtt ccccttcggg ctgaaatgaa
     1201 agatgacagg aaaaaacggg tgccgcgaat gtggcaccct gaggttagtt actggcctga
     1261 agccagcgga accggtaaag gcgcggcttc tgtctgttct tcagcgcgct cctccaccag
     1321 atacagccgg ggctgtggaa gtttttccct ccccggccat tcggcctgcg ggtcactgtc
     1381 cgtatgttca ccaccgtcat aacagttgta accctgtatg ccccggacag gttcgctgta
     1441 ccagtgccgt atctcacagt cgaacacctt cgagatttca ccaaccagct cacctgaagg
     1501 cggataccag ggcgtgtctg tcagcagcac cagactgctc atctcatcac gcacccagcg
     1561 gacattgtga cctgatggcc actccacacc gtatatccgg ctgtaaaggc tggtcgtggt
     1621 ggaaatgccc tgcagcagtc cacctgaacc gttcagctct gtggccagac gggggggtat
     1681 gaccatcagc atgtcacagg gctgcgcctg ttccgggtaa tccgacagcc ggctccagca
     1741 ggcttcggca tcaatgtgtt cactcagtcc gaccagacca aaccagtcag aatactgaca
     1801 atgcattaac gtggtgatta tctcccgggc attatccggc agattccccc agcggactgc
     1861 gccaatacat gactggtgat aaaggcggtc aatggtcttt atcgtctctt catccagtac
     1921 ggcatctttt accagcagcc ccagccactg ttcaaatgcc tggttagccg gagatgacat
     1981 ccccgtacca tgacgaacca gcgccggata tggcggatac tgcgtcgtcc gggtaggttt
     2041 caccagtccg gcacaaccga tcagaaacag ctgtatactc tgctgtatgg catggcggta
     2101 acgcggtgtc tcacatcctt caatccactg cgtcagcaca tccaggcaga ctgacttacc
     2161 ggtaatttca aaacgatttc tgcaccattc ggccatattc aatgctcctc tgataaatga
     2221 agagggcatt gtcccgtgag gtacaatgcc ctcacgggaa aagcggagtt gtcacactcc
     2281 gccatcgtca aaagtccgtc cggttataca accttccggt atttactggt ttctgtcccc
     2341 atttcgttga tagctacgtc gatattgccc gggaaacgat ctgttttaat cttccacaaa
     2401 tcgtttttcc agtaacgtgc cactgtcaca tctggcggaa cgttgctctg cccgtacttt
     2461 ttacgaagcc ggtcaatgca ctcctgcaca aattcattac tgaatttgcg ggattcgtgc
     2521 acgaacctca gcccgttttt gccttcaaac gcacgggtaa taaaccgcac ttcagcctct
     2581 gttggtccat ccagccagga cacattaatc atgttgtaaa cgccggaatc gcataccttc
     2641 agcacgttaa atttctgccc cggaaatgcg aattccagaa cgtcactgac acgggcaacc
     2701 acggcatcgt caacctcagt cacagactta acattcatca ttctgctcct ctgtatgaaa
     2761 atcactgagg gaacagcccc gtgaggagcc gggccctcac gggatggcgg tgtgtcacac
     2821 cgcgttactg ataaagtctt tcagccaggt ggtatgcccg aggccatacc gcctgctggc
     2881 attcataagt tcgtctgccg acggatttat gtaaaggttg tagtaccaga gttcttcctt
     2941 taaatccgcc aggttacccc tgccggtcgc aagccactcc tgcaccacgt catgaatttc
     3001 agatgtgacg tcaccgcggt aattcgatgt gatcgtaaca tccgtcagta acccggcaat
     3061 actgcccgcc agcagacgcg gttgagacat gccgttatag aaccatacgc tgcagccgga
     3121 atctccgggt aaaatattca aatcgggtct catgaaattt ctcctgaaaa agcagggacc
     3181 cgtcccgcag ggatgagtcc ctgtgggaat gtaaaaaccg ttaaaatgca tctggtattg
     3241 cagcgtaccg cttcccggta ctgatactgt cagcccagat actgtgctct gtttcatgtc
     3301 ccagagacat aaccctgtcg agtacctcat ggaaaatcgt tcccggtgct ggagcatttt
     3361 ccatctccag gtcatacagc aaccagccca gagcctcacc aacatcccac aaaatcgagt
     3421 aaagtggtga aggctcatcc tcatcctccg gcaccggggt aattccgaga cgttgccagg
     3481 cgcgaagtat cgccagttca ccttccagct gacagagatg tgccagcaaa gcggactgac
     3541 tctgaagatg ccagtaacca gtaataaaac gcgaatacat aaaaccccct gtctgattct
     3601 ttctgatgaa agagcacacc gtccgaagga acgatgccct ccgggcggtg aacaacagag
     3661 ttcagattgt tcacccgcga ttgttaagca ttacgccgct ttttctgccg gcgtatcgtg
     3721 tacggaaaga aggtattccg ttgcctctct ggcctgtcga catgcactga acagcgcttt
     3781 tttgtcagca cgaagaattt tcagccatgc gccaatataa ttctcatgct gcacatttcc
     3841 tgtgacaccc agttgagcgc acagaaaggc actgcccatt tcagcgataa gctcttcgaa
     3901 cgcataacat tcatcgccat agcgcccacc tgaggaaact atcccttccc ggttaagacg
     3961 tttcgcatga ccggtactgt gtaccagctc atgcagtaat gtggaccagt aatcttccgc
     4021 tgaagtgaac tgtgacgaga gcggcaggac gatacgatca aataccgggg tgtaatacgc
     4081 ccggtcctga ggtaaagaat gaatcttcac accgcaggca ttcaccatcg tcagtacacg
     4141 atcagccaca gcattcagcg tgtcgttcgc accgttctca gtgacggttg tcgtcacctc
     4201 cggcagcgac aatccatcac actgttcaac gttaaagaga ggattcgctt tcagcaccgg
     4261 tacatcaacc ataacggggt taccctctgc gtcgaatatc cggttgccgt tctcatcctc
     4321 atcctgctta tgccagcttt tgtatatcac ggccatcgtg gacttctcgc ccttacggac
     4381 ctgcccaccc agcgcctgag cctgacggta cgtcaaccag cagttactgc tgtaaccggc
     4441 ttcttctgcg gcgagccaca acagcaacac attgatccca cggtaagcgc tacgtgtcag
     4501 tgcattaacc ggcatcattg gatcgccgct gtgagtccgt actgagcgcc atggacgttg
     4561 ccagggggct acgccctttt cgatggcctc aatcaccctg tccgtcacca gctgatacag
     4621 gtcacgctcc ggggttttat tcacgggaca tgaagtatgt tttttcgttt tcataagagc
     4681 acgctccata aaaaagatgc atgctccccg tacggtgaac atgcaccata cggttaaatt
     4741 aataacgaaa atcagactgg ccggacttac cacaacgcca gcatcaccag tgaatgacgc
     4801 tcaaaatcaa acgtcttcag cgtatcgctg acccacgcca ccgacgcagg tacacggtac
     4861 agtaagtaaa agaaaaaccg ttcagtctgc gtcggaaaag tcaatacaga ccgccttcag
     4921 gagaccggct atacgttgat ccagacattc agtccagcca tcgttccatc cacggtccca
     4981 tggattgtag acatcccccc atggattgca gacatcgtta cagccattct tccatgcctg
     5041 ccataccgcc atttccatga cagctcccag cctgtggcct ttcgcacgta tatgccacag
     5101 aaacggccgg aaaagtcctg atttcagttg gtcaccaccg ctttcgtagc ccagatgacg
     5161 agcaatactt tcaagatatt caaatgcatc attctgcatt ccatccgcct tagccagcga
     5221 gctgtacgca tcacgaagta tcgtcatacc ttccagttga tactcacacc acgcacggac
     5281 agacgacact ggtgcgatta cccgaagaat ggtgcaggca cggcatacaa caacgtaaag
     5341 gagtattaac agactattca tgtgttcacc tcagatacaa aaaattacgg gaacacacct
     5401 gccctacggg acacatgttc ccgacagggt aataaggtta aaaacaagga cagaaaaata
     5461 attaccacca tgcccagagc aacagtattt ttcgttcaaa atcaaacgaa tcaagcgtgc
     5521 ttctgaccca cgatttcacg atctcaacat ccttccagta atactggtca tactcctgag
     5581 aaccaaaaaa gaatccttct gttgtgggga agaattcccg gcagttctcc ggcgtaaggc
     5641 attcaagatc gctcagtagt gcctccagtt ccgttctgga aaccggacgc tccacggcat
     5701 tttcaaccgg tccacaatgc gtctcaaacc aggcaatcag tgggttaacc ttgcgaaagt
     5761 aacccacttc ccgcgtttcc ggtaccgata ctaccgcgtc ggtaacacga tcacgcccaa
     5821 ggaaaaaaat atcgagtccc ataaacaatc tcctgataaa aacagcggga ccgtccccga
     5881 acgggagcgg tcccggacgg gttaaggaaa actcagattt gtcatcgcgc acgcgtggta
     5941 cggtcagaca gatccgggcg ttgtgtgata aaccttccaa cggtcatgac gaccgttcaa
     6001 caggcttcag cgaagaccga agcgatagcg aaaagaagta agctgcgcgg ccatacggga
     6061 ggcagcagag agcgtgcggc gagcggatat tcgaccggta aagacattaa accgcacgac
     6121 agacagcgga agtgcctcaa accagtaacg atattcacgg ctgtgagagc cttcacggaa
     6181 ctcaaagcag tgtcgcaatg caccgttggt ggaaacaatg caggctctga ctgacagatt
     6241 gccggtggcg ttaataaacc acggagtgca gtacagatgc ctcagttgtg gagcggacca
     6301 gggctgttgt gggtgaacac aacgcttctg attaaacaga cattgtctga gaagatgaaa
     6361 gaaaggcgtt atgcgcatat ctgcacgtaa caaaagtggt ataaacatcg gaacctccga
     6421 atgaaaaaga cggacgttcc gccctgacgg actggaaaat ccgtcaggtg aacacatcaa
     6481 tgcgtcagca cgatgcgctt aagaagacca gagacaactc atagtcacat tgtatacaaa
     6541 gtgccttcat catcacgtaa aaccgtggtg acatctgctc tcaaaggtgt gcagatttgc
     6601 ccgtacatca gggatgtttc ccttcagtac tcacagctcc tttcaggcta cggaggtgta
     6661 aaactgacca cccgaaggcg ctcctcacag gtcatatgga gcattggtgg tcgccggagc
     6721 gacgtgggcc acccgaaggc gttcctcaca ggccacgatg gaacattggt ggtcgccgga
     6781 gcgacaagtt agcgtctaaa atataatcat aaacaacata aaacttcaag ttgttgtcgt
     6841 ggctatggta ataacctaca actgactgac aaggccagct caatttgata ttttttaatt
     6901 cagtaacact ctttatgaac ctgttagaga gatgtgaaaa tgaaatcgaa atacaaaaaa
     6961 ttaaaagatg aactactcag aatagccaaa gcatgcgccc cgacgccaga agatatgttg
     7021 gtatatacgg gaagagccag aagacttgct tcatttctta aagatgccaa tatacagata
     7081 agctcagcaa atagcataaa actacgtcat attgaatgct attttcaaca acgttatcgc
     7141 actggcgtca gttccaaaat actacgagaa gaattagata caataaagca tgttctcacc
     7201 cattgcggaa agagaaacat cgttaaaaat gaacgactga cttatacatc actgaatatt
     7261 gcagatgtcc gaccaattgt tatctgccca tactgtggaa ataagacgaa tcttataaaa
     7321 ggggctctca tgccattttc catttcagcc gcgacagaaa acaaatatta ctggatttgt
     7381 cccccatgca atgcatgggt tggatgccat aaaaactcag gaagaccatt aggtactccg
     7441 gctaaagaaa acctgagaat actcagaaca aaagtacgta aactttttga caattatcag
     7501 caaagagcca acatatcccg taacgaggca aacatatggc tatccagaaa actgaattgt
     7561 cacattcatg aatgtcatat tgggtatttt gatgaagata tgtgtaatcg cgcatcagaa
     7621 atcatcataa ccgaaattaa caaaaacaca tatcctccag attcatttta aatatcaact
     7681 ttcatcagaa ccgttaaaat aaacctgttt tacataacga cctcttccgt caccatggcc
     7741 aagatccata gaaacacgag cgtagatttc cgactctcca tatccatttt ttcgataaaa
     7801 ttttctggct tcctgagaaa aatgataacg catactgtgc ggagatttca ctcctgacaa
     7861 tcccgcttta cgggcatgat aacgaaaaac attgatagcc tgttttatat ttggacggtc
     7921 aataagtttt ccgtcgtgtt catccattat tttttgagca taaagcaaag catgtcttac
     7981 cgcatctctg tcaataattg tggtatttct tggtcttcca cctttcgtac caaaaacgac
     8041 cgaaagagaa tcgcaccctg aattcagttc ccgtagccat gactgaacag acttacatga
     8101 ctgaacggct tcttttgttc ttaaacccag aacataagaa agctgcattg cagctgccac
     8161 accaggattc tttttttcca ctttagcaaa tgctttccag aattcatccg gtgacactgc
     8221 tattttcttt ccatcacggc atgtatcctt taggccgagc gcttcattac tcaataatgg
     8281 gttagaagga tctgcgattt taagtcgtcc atctttttct aaaatggcac gcataaccgc
     8341 ccgttcattc tgaatagtcc ggtgagaaat acccgctgct ttcctgctga gaaggtaacc
     8401 agaaatatgt ctggttttaa tactgctcgt ctggcgaagt ttaattccat tttcacgtaa
     8461 ataattcagg aagtactttg caattctcat tctgtcagct gttgttgcgt aactgccacc
     8521 acactcacgg gcaagtgaga tgaaagactt gcgaaacagt tcaactttag aacgtgacga
     8581 tttccgggcc ataaagtcat tctccctgta aaaatcagat gcagccccct tccgttcaca
     8641 aagtaaaact gcatacgcag cccttccggt aaacagaaaa gttcgttgat attgttagtt
     8701 tttcgtcatc acggacggct ctgtaacgcg gttgttcgcc ttcctggctc gtttgatgtc
     8761 gatgtagcag agcttgcaca tgttgagttc ttacaggagt tcgctacctg tgcaccaggg
     8821 cgattgttga ggaatactcc ccgtcacctt acgatgacgc ctcccgacat cagaacgatt
     8881 tttcattttt attcctttat ttcagtcagt taatacactt acctctcatg tattacgaag
     8941 tttaagaaga cattccggcg tggccgtccg tgtgaaaacg gacgcgccgg aaaggcgtgg
     9001 atacgtcacc gaaagacgtt gtcagagcga caaaacgagc atcaggacgc ttgtcacgag
     9061 cgacgatttt ctgccagacc ttacgtgatg ctgccttcag ggcagatggc cgcggcgtca
     9121 cttggtgttc ccttcggtcg cacgcaagtg acgccgcaca gcttcaaact ccacatgcac
     9181 acgcaagcga ccaaatttca aatggtctta tgccgtgcgc acagtaataa gaagctcatg
     9241 cggaaagtca gaaaatatct taagacgcag attttaaagg cgtttttatg cctgtctgct
     9301 gagaaaaaat aaaagtaccg gaacggaatc cggaatgcct tcatcatcac gtaaaaccgt
     9361 agtgacatct gctctcaaag gtgtgcagat ttgcccatac atcagggatg tttcccttca
     9421 gtactcacag ctcctttcag gctacggagg tgtaaaactg accacccgaa ggcgctcctc
     9481 acaggtcata tggagcattg gtggtcgccg gagcgacatg ggccacccga aggcgttcct
     9541 cac
//
LOCUS       DAZGVQ010000189         9354 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000189 DAZGVQ010000000
VERSION     DAZGVQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.190"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..606)
                     /gene="upp"
                     /locus_tag="ACHLAG_004527"
     CDS             complement(<1..606)
                     /gene="upp"
                     /locus_tag="ACHLAG_004527"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFP0657640.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGD"
     gene            931..1968
                     /gene="purM"
                     /locus_tag="ACHLAG_004528"
     CDS             931..1968
                     /gene="purM"
                     /locus_tag="ACHLAG_004528"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFP0657641.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNSNGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            1968..2606
                     /gene="purN"
                     /locus_tag="ACHLAG_004529"
     CDS             1968..2606
                     /gene="purN"
                     /locus_tag="ACHLAG_004529"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFP0657642.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            2777..4843
                     /gene="ppk1"
                     /locus_tag="ACHLAG_004530"
     CDS             2777..4843
                     /gene="ppk1"
                     /locus_tag="ACHLAG_004530"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFP0657643.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDFIK
                     SLEQPE"
     gene            4848..6389
                     /gene="ppx"
                     /locus_tag="ACHLAG_004531"
     CDS             4848..6389
                     /gene="ppx"
                     /locus_tag="ACHLAG_004531"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFP0657644.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(6428..8671)
                     /gene="pdeF"
                     /locus_tag="ACHLAG_004532"
     CDS             complement(6428..8671)
                     /gene="pdeF"
                     /locus_tag="ACHLAG_004532"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFP0657645.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLDKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(8853..9005)
                     /locus_tag="ACHLAG_004533"
     CDS             complement(8853..9005)
                     /locus_tag="ACHLAG_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657646.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            9023..9214
                     /locus_tag="ACHLAG_004534"
     CDS             9023..9214
                     /locus_tag="ACHLAG_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFP0657647.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT         2422 a         2300 c         2283 g         2349 t
ORIGIN      
        1 gtcaccggca tcgccgaggc ccggaataat gtatccgtgc tcgttcagtc cctgatcaat
       61 cgatgcggta tacagttcga cgtccgggtg cgctttttcc agcgcagcga taccttctgg
      121 cgcagcgacc agcaccagaa ctttgatgct gctgcagccc gcttttttca gcaggtcgat
      181 ggtcgcgata acggaaccac cggttgccag cattgggtca acgatcagcg ccatacgctc
      241 atcgatgtta gaaaccagtt tctggaagta cggtaccggc tccagcgttt cttcattacg
      301 gtacataccg acaacgctga tgcgcgcgct cggaacgttt tccagcacac cgtccatcat
      361 accaagaccc gcacgcagaa ttggcacaac ggtaattttc ttacctttga tctggtcgat
      421 ttctaccggg ccgttccagc cttcgatagt tactttttcc gtttcgaggt cggcggtcgc
      481 ttcgtaagtc agcaggctac ccacttcgga agcgagttcg cgaaagcgct tggtgctgat
      541 atcttgctca cgcatcagtc ccagcttgtg tttgacgagt gggtgtttga cttccacgat
      601 cttcatactc tttctccttt gaggggcagc cacaaaaaaa atcgacggat tatacctcct
      661 ttcttcaagg cggcaatatt cttttcgttg actttagtca aaatgataac ggtttgagat
      721 aaagttattt tatattcaaa tggttatgaa agaagattat tccatccgaa aactaacctt
      781 taccctggca caagtcttct ttcgccgcgt gcctgggcaa aagacgtgca aaaaggttgt
      841 gtaaagcagt ctcgcaaacg tttgctttcc ctgttagaat tgcgccgaat tttatttttc
      901 taccgcaagt aacgcgtggg gacccaagca gtgaccgata aaacctctct tagctacaaa
      961 gatgccggtg ttgatattga cgcgggtaat gctctggttg gaagaatcaa aggcgtagtg
     1021 aagaaaacgc gtcgtccgga agtgatgggc ggtctgggcg gcttcggtgc gctgtgtgca
     1081 ttgccgcaaa aatatcgtga acctgtgctg gtttccggta ctgacggcgt aggtaccaag
     1141 ctgcgtctgg cgatggactt aaaacgtcac gacaccattg gtattgatct ggtcgccatg
     1201 tgcgttaatg acctggtggt gcaaggtgca gagccgctgt ttttcctcga ctattacgca
     1261 accggaaaac tggatgttga taccgcttca gcggtgatca gcggtatcgc ggaaggatgt
     1321 ctgcaatcgg gctgttcact ggtgggtggc gaaacggcag aaatgccggg gatgtatcac
     1381 ggtgaggatt acgatgtcgc gggtttctgc gttggcgtgg tagaaaaatc agaaatcatc
     1441 gacggctcta aagtcagcga cggcgatgtg ctgattgcac tcggttccag cggtccgcac
     1501 tcgaacggct attcgctggt gcgcaaaatt cttgaagtca gcggttgtga tccgcaaacc
     1561 accgaacttg atggtaagcc attagccgat catctgctgg caccgacccg catttacgtg
     1621 aagtcagtgc tggagttgat tgaaaaggtc gatgtgcatg ccattgcgca cctgaccggc
     1681 ggcggcttct gggaaaacat tccacgcgta ttgccagata atactcaggc agtgattgat
     1741 gaatcttcct ggcagtggcc ggaagtgttc aactggctgc aaacggcagg taacgttgag
     1801 cgccatgaaa tgtatcgcac cttcaactgc ggcgtcggga tgattatcgc cctgcctgct
     1861 ccggaagtgg acaaagccct cgccctgcta aactccaacg gtgaaaacgc gtggaaaatc
     1921 ggtatcatca aagcctctga ttccgaacaa cgcgtggtta tcgaataatg aatattgtgg
     1981 tgcttatttc cggcaacgga agtaatttac aggcaattat tgacgcctgt aaaactaaca
     2041 aaattaaagg caccgtacgg gcagttttca gcaataaggc cgacgcgttc ggccttgaac
     2101 gcgcccgcca ggcgggtatt gcaacgcata cgctcatcgc cagcgcgttt gacagtcgtg
     2161 aagcctatga ccgggagttg attcatgaaa tcgacatgta cgcacccgat gtggtcgtgc
     2221 tggctggttt tatgcgcatt ctcagcccgg cgtttgtctc ccactatgcc gggcgtttgc
     2281 tgaacattca cccttctctg ctgccgaaat atcccggatt acacacccat cgtcaggcgc
     2341 tggaaaatgg tgatgaagag cacggtacat cggtgcattt cgtcaccgat gaactggacg
     2401 gtggcccggt tattttacag gcgaaagtcc cggtatttgc tggtgatacg gaagatgaca
     2461 tcaccgcccg cgtgcaaacc caggaacacg ctatttatcc actggtgatt agctggtttg
     2521 ccgatggtcg tctgaaaatg cacgaaaacg ccgcgtggct ggatggtcaa cgtctgccgc
     2581 cgcagggcta cgctgccgac gagtaatgcc ccgtagttaa agcgccagct ctgccgctgg
     2641 cgtttttcaa ttcacctgta aatcgcaagc tccagcagtt tttttccccc ttttctggca
     2701 tagttggaca tctgccaata ttgctcgcca taatatccag gcagtgtccc gtgaataaaa
     2761 cggagtaaaa gtggtaatgg gtcaggaaaa gctatacatc gaaaaagagc tcagttggtt
     2821 atcgttcaat gaacgcgtgc ttcaggaagc ggcggacaaa tctaacccgc tgattgaaag
     2881 gatgcgtttc ctggggatct attccaataa ccttgatgag ttctataaag tccgcttcgc
     2941 tgaactgaag cgacgcatca ttattagcga agaacaaggc tccaactctc attcccgcca
     3001 tttactgggc aaaattcagt cccgggtgct gaaagccgat caggaattcg acggcctcta
     3061 caacgagctg ctgctggaga tggcgcgtaa ccagatcttc ctgattaatg aacgccagct
     3121 ctccgtcaat caacaaaact ggctgcgtca ttattttaag cagtatctgc gtcagcacat
     3181 tacgccgatt ttaatcaatc ctgacactga cttagtgcag ttcctgaaag atgattacac
     3241 ctatctggcg gtggaaatta tccgtggcga taccatccgt tacgcgctgc tggagatccc
     3301 atcagataaa gtgccgcgct ttgtgaattt accgccagaa gcgccgcgtc gacgcaagcc
     3361 gatgattctt ctggataaca ttctgcgtta ctgccttgat gatattttca aaggcttctt
     3421 tgattatgac gcgctgaatg cctattcaat gaagatgacc cgcgatgccg aatacgattt
     3481 agtgcatgag atggaagcca gcctgatgga gttgatgtct tccagtctca agcagcgttt
     3541 aactgctgag ccggtgcgtt ttgtttatca gcgcgatatg cccaatgcgc tggttgaagt
     3601 gttacgcgaa aaactgacta tttcccgcta cgactccatc gtccccggcg gtcgttatca
     3661 taattttaaa gactttatta atttccccaa tgtcggcaaa gccaatctgg tgaacaaacc
     3721 actgccgcgt ttacgccata tttggtttga taaagcccag ttccgcaatg gttttgatgc
     3781 cattcgcgaa cgcgatgtgt tgctctatta tccttatcac acctttgagc atgtgctgga
     3841 actgctgcgt caggcttcgt tcgacccgag cgtactggcg attaaaatta acatttaccg
     3901 cgtggcgaaa gattcacgca tcatcgactc gatgatccac gccgcacata acggtaagaa
     3961 agtcaccgtg gtggttgagt tacaggcgcg tttcgacgaa gaagctaaca ttcactgggc
     4021 gaagcgcctg accgaagcag gcgtgcacgt tatcttctct gcgccggggc tgaaaattca
     4081 cgccaaactg ttcctgattt cacgaaaaga aaacggtgaa gtggtgcgtt acgcacacat
     4141 cgggaccggg aactttaacg aaaaaaccgc gcgtctttat actgactatt cattgctgac
     4201 cgccgatgcg cgtatcacca acgaagtacg gcgggtattt aactttattg aaaacccata
     4261 ccgcccggtg acatttgatt atttaatggt gtcaccgcaa aactctcgcc gtctgttata
     4321 tgaaatggta gaccgcgaaa tcgccaacgc gcagcaaggg ctgcccagcg gtatcaccct
     4381 gaagctaaat aaccttgtcg ataaaggcct ggttgatcgt ctgtatgcgg cctccagctc
     4441 cggcgtaccg gttaatctgc tggttcgcgg aatgtgttcg ctgatcccca atctggaagg
     4501 cattagcgac aacattcgtg ccatcagtat tgttgaccgt taccttgaac atgaccgggt
     4561 ttatattttt gaaaatggcg gcgataaaaa ggtctacctt tcttccgccg actggatgac
     4621 gcgcaatatt gattatcgta ttgaagtggc gacgccgctg ctcgatccgc gcctgaagca
     4681 gcgggtgctg gacatcatcg acatattgtt cagcgatacg gtcaaagcac gttatatcga
     4741 taaagaactc agtaatcgct acgttccccg cggcaatcgc cgcaaagtac gggcgcagtt
     4801 ggcgatttac gacttcatca aatcactcga acaacctgaa taaccctatg ccaatacacg
     4861 ataaatcccc tcgtccgcag gagtttgctg cggtcgatct tggttcaaac agttttcaca
     4921 tggtcatagc ccgtgtggta gatggtgcca tgcagattat tggccgcctg aaacagcggg
     4981 tgcatctggc ggacggcctg gggccagata atatgttgag tgaagaggca atgacgcgcg
     5041 gtttgaactg tctgtcgctg tttgccgaac ggctacaagg gttttctcct gccagcgtct
     5101 gtatagtcgg cacccatacg ctgcgtcagg cgctgaacgc cactgacttt ctgaaacgcg
     5161 cggaaaaggt cattccctac ccgattgaaa ttatttccgg taatgaagaa gcccgtctga
     5221 tttttatggg cgtggaacat acccaaccgg agaaaggtcg caaactggtt attgatattg
     5281 gcggcggatc tacggaactg gtgattggtg aaaatttcga accgattctc gttgaaagcc
     5341 gccggatggg ttgtgtcagc tttgcccagc tttacttccc tggcggggtc atcaataaag
     5401 agaattttca gcgcgctcgt atggcggcag cacaaaaact ggaaacttta acctggcaat
     5461 tccgtattca gggctggaac gtggcaatgg gcgcttccgg taccataaaa gccgcccatg
     5521 aagtgttaat ggaaatgggc gagaaagacg ggataattac cccggaacgt ctggaaaaac
     5581 tggtaaaaga agttttacgt caccgtaatt tcgcatcgct gagtttaccg ggtctttccg
     5641 aagagcggaa aacagtcttc gttcctggac tggcgatttt atgcggtgtg tttgatgctt
     5701 tagccatccg tgaactgcgc ctttctgacg gggctttacg cgaaggcgta ctgtatgaaa
     5761 tggaaggacg tttccgtcat caggatgtgc gtagtcgcac cgccagcagc ctcgccaacc
     5821 agtatcacat cgacagcgag caggcccgac gggtgctgga taccactatg caaatgtacg
     5881 aacagtggcg ggaacagcaa ccgaagctgg cgcatccgca actggaggcg ctactgcgat
     5941 gggccgccat gctgcatgag gtcgggttga atatcaacca cagcggtttg catcgccact
     6001 ccgcttatat tctgcaaaac agtgacttgc cgggttttaa tcaggaacag cagctgatga
     6061 tggcgacact ggtgcgctat caccgtaaag cgattaagct cgacgatctg ccgcgcttta
     6121 ccttgtttaa gaagaaacag ttcctgccac tgatacagct attgcgcctt ggcgtattac
     6181 taaacaatca acgtcaggca accaccacac cgccaacatt gacactgatt actgatgaca
     6241 gtcactggac actgcgtttc ccgcatgact ggtttagtca gaatgcgctg gtactgcttg
     6301 atctggaaaa ggagcaagaa tactgggaag gcgtggctgg ctggcggttg aaaattgaag
     6361 aagaaagtac acctgaaatc gccgcttaat gctttgcggg ccgacgagaa atgtcggccc
     6421 gcattattca ggcactttcg cgaatgggtt cgatttcatt cagcgtatca attaacggtt
     6481 gcggcttacc aataagataa ccctgcatat aatcgatccc caaagagagc accgcctcgc
     6541 ggatctcttc gttttcaacg tactctgcca ctacccgcat tttcttcatt cgcgccaggt
     6601 ggcaaatcga tgccactatc tgataatcca gactatttga cacaatattg cggataaaac
     6661 tgccgtcaat tttaagcaga tcggcattca catttttaag ccgcgcatag ctggcgtagc
     6721 cggtgccgaa atcatcaatc gcaatctggc agcctaattc ctgaagatgc tgcaaggtga
     6781 tttgcgcctg cttaacattg gtcagagcat tactttcggt gacttcaaaa ataagttgcc
     6841 acgcttcaat ctgatattta tccagcaact gactgacttc aacaggaaaa cgagcctgac
     6901 ataccgaggt tggagacaga ttaatagcaa aacggtgagc gggcatcttc gctctgtttt
     6961 cagccataaa ttgcagcgta tgctcaatga cccacatgtc gatactcgac gataaaccaa
     7021 attcgtgcgc gaccggcaag aagctatcgg ggccgatcag ttcatcattc tcacctttca
     7081 tgcgcagaag aatttcatgg taaacatcac cacgcatacc ggtaatcggc tgggccatca
     7141 ggaaaaaatg gttgtgttcc agcgcctgct gtagtcgatt catcatcgcg actttatctt
     7201 tcaattcgcg ttgcaaatac attgccccgc gacgctgcat attttccggg gcgttggtca
     7261 cgatggaaag ttcggcgacc gtatttagct ctcccagcag caggtagata tgattcactg
     7321 gcgagcgcac atagcagtaa ctgacgccaa tctgcggttg catcggcatg ccatcccaaa
     7381 agaaacgaaa ttgcttgaga tggctatcca gtgcggtaat gcgctcctgg tgcgattctg
     7441 tattcagccg cagcgcgaga tcgttacccg aaagctgata aacatcttca cccggttcca
     7501 gcaagggtga cagccagtga gaaagttttt gcttgtattg aatccgcagc atgatgccat
     7561 agttcttaac cagcatttcc atgccaggga tgcgtaaata acaaagcgca gaccaggggg
     7621 catcacgcaa cgcgcgattc agggcgcgaa catttggcag atgaaccacc gggtccacat
     7681 acgcaagccg ctgaatgcgt ctgactaccg ctcgctgacg agttgccagt acagccatat
     7741 aattgacaat aaaagagaat accagataac tggaggaggt tatggtcagc tgcgtggtat
     7801 agccaggata aatgggaatg taattttgat agctgtggat gctgatcatc aacacgaccg
     7861 cccagagcag cgaaatcagc ttataaccat agcgcatcgc tccccacatc atcaggggca
     7921 gcaataatga caaggtataa ttggtgctaa aaattgtgct tttttcattt aacggcatgc
     7981 ataacagcaa caataaagca cctaatgcca gtagccagag cgcgaactct tttttggtga
     8041 ctttggcatc aacctgctgt tttaattgcg aataatagct acgtaaataa aatggatttc
     8101 gcactacccg aatgatgaag tagcacagcg ggacaccgat cagattaccc accagtaagg
     8161 cctgataatt gattaaagtc ccgaggttaa aaggcatgac accgaccaga ttttctctgc
     8221 tcgccagcaa tcctacaaat gcagcaaact ggaaaagtat cagaaacagc gttgcaggaa
     8281 acactatctg ccagaatata cgctgggaaa ttaaacgggt atcaccatgt gaaacgttgt
     8341 tacgccgggg agtaaagacc ctgtaaccgc cccaacagag aatgataatg ataataaaat
     8401 tagccgttaa tgagagcgtt tcgtaaaaac ccagctctgc atatttgcgg acaaaaatcc
     8461 ctaaggtgat ccctggcaat gccgcccagc tgaaaaacat catcatgctg atcatcagtg
     8521 ccaaaggcag ataaaagaga aaaacctcac cggaagaaat atgcgcgaaa gtattaatgt
     8581 gggcgaaaat gggcaagatt aaagaaggca ggaacagcgg aagcccccac catttatcac
     8641 gtatttttat ataagttgca ttcagtttca tagatgctca gcagaatccc ccacatcctg
     8701 aaggaggtgt attcagacag gcatcccacc tgacttcgaa tgatgattat tcatcactat
     8761 agagagcatt gattctaagt gtcatatgaa agttccaatt gatatatatc aaacaaaata
     8821 accctgatta atgaattatt acgtttatca tgttaattca tcattattac atcatcattg
     8881 taaataatta aattaacttc cataacatta aaatatgtat ccactgacgc ttttttacat
     8941 aacgaagaat tgaccatttt gtcctgttgt gccttaatgt aagtaccgtc cacagcgtgg
     9001 gacatacttc aaggaacctt ttgtgagtca ggcaaccagt atgcgaaaac gacaccgatt
     9061 taacagtcgc atgacccgta tcgtactgct catcagcttt atcttcttct ttggccgttt
     9121 tatctactcg tccgtcggtg cctggcagca ccatcagagc aaaaaagaag ctcagcaatc
     9181 cacactctcc gtcgaatcac cggtacaacg ttagcggtta ccttctccac tttcacagaa
     9241 cctaacggca cttcgctgtc ggatgctttt gctctttgga attatcaaag cggcagatat
     9301 tctttcatct taaattttac gtctttgtcc tgactgatgt ttatcctgtt tggc
//
LOCUS       DAZGVQ010000190         9203 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000190 DAZGVQ010000000
VERSION     DAZGVQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.192"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..253
                     /locus_tag="ACHLAG_004535"
     CDS             <1..253
                     /locus_tag="ACHLAG_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657648.1"
                     /translation="FILFSSVDDGPVMVVSSQRLHDMLNPTKDTNWNSTYIYKSRHEI
                     LPVNLTPETLFGSKSQDKHALFPIFTASWRATRIKNKGI"
     gene            476..973
                     /gene="ubiC"
                     /locus_tag="ACHLAG_004536"
     CDS             476..973
                     /gene="ubiC"
                     /locus_tag="ACHLAG_004536"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFP0657649.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            986..1858
                     /gene="ubiA"
                     /locus_tag="ACHLAG_004537"
     CDS             986..1858
                     /gene="ubiA"
                     /locus_tag="ACHLAG_004537"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFP0657650.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2013..4436)
                     /gene="plsB"
                     /locus_tag="ACHLAG_004538"
     CDS             complement(2013..4436)
                     /gene="plsB"
                     /locus_tag="ACHLAG_004538"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFP0657651.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPVHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEEYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4607..4975
                     /gene="dgkA"
                     /locus_tag="ACHLAG_004539"
     CDS             4607..4975
                     /gene="dgkA"
                     /locus_tag="ACHLAG_004539"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP0657652.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5085..5693
                     /gene="lexA"
                     /locus_tag="ACHLAG_004540"
     CDS             5085..5693
                     /gene="lexA"
                     /locus_tag="ACHLAG_004540"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFP0657653.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5766..7091
                     /gene="dinF"
                     /locus_tag="ACHLAG_004541"
     CDS             5766..7091
                     /gene="dinF"
                     /locus_tag="ACHLAG_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFP0657654.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7207..7416
                     /gene="yjbJ"
                     /locus_tag="ACHLAG_004542"
     CDS             7207..7416
                     /gene="yjbJ"
                     /locus_tag="ACHLAG_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP0657655.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7458..7973)
                     /gene="zur"
                     /locus_tag="ACHLAG_004543"
     CDS             complement(7458..7973)
                     /gene="zur"
                     /locus_tag="ACHLAG_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFP0657656.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8291..8545
                     /gene="yjbL"
                     /locus_tag="ACHLAG_004544"
     CDS             8291..8545
                     /gene="yjbL"
                     /locus_tag="ACHLAG_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFP0657657.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            8569..>9203
                     /locus_tag="ACHLAG_004545"
     CDS             8569..>9203
                     /locus_tag="ACHLAG_004545"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFP0657658.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DATPIERCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYTDLAGAEKVNINEYNENYLTAQFEELSQRDMKKYLGEM
                     KHLADRGGMKFDDYLDKMSLLVNEGKLEPDILSKMRDAAPKLIDFAKSFDP"
BASE COUNT         2192 a         2150 c         2447 g         2414 t
ORIGIN      
        1 atttatcctg ttctcctctg tcgatgatgg gcctgttatg gtggtaagct cccagcgctt
       61 acatgatatg ttgaatccta caaaagatac caattggaat tccacgtata tctacaaatc
      121 cagacatgag atattgcctg ttaatcttac tccggaaaca cttttcggct ccaaatctca
      181 ggataaacat gcacttttcc ccatttttac tgcgagttgg cgagctactc gtataaagaa
      241 taaaggtatt taagtaaagg aaaacatcac cattcctggc atcatgggcg gtgatgcccc
      301 tatggttgcc ctcgccagca cgggcatcgg caaagcgtaa ggttcaacag cgttttacca
      361 cttcatgcga ttgttgcgtt tttgttgcgc attagatcac ttaatttgct ttacatctcc
      421 cgtaaacact tttctgcgat acaatgcctt tacgttatgt aacggagagt tcggcatgtc
      481 acaccccgcg ttaacgcaac tgcgtgcgct gcgctatttt aaagagatcc ctgccctgga
      541 gccgcaactg ctcgactggc tgttgctgga agattccatg acaaaacgtt ttgaacagca
      601 gggaaaaacg gtgagcgtga cgatgatccg cgaagggttt gtcgagcaga atgaaattcc
      661 cgaagaactg ccgctgctgc cgaaagagtc tcgttactgg ttacgtgaaa ttttgttatg
      721 tgccgatggt gaaccgtggc ttgccggtcg taccgtcgtt cctgtgtcaa cgttaagcgg
      781 gccggagctg gcgttacaaa aattgggtaa aacgccgtta ggacgctatc tgttcacatc
      841 atcgacatta acccgggact ttattgagat aggccgtgat gccggactgt gggggcgacg
      901 ttcccgcctg cgattaagcg gtaaaccgct gttgctaaca gaactgtttt taccggcgtc
      961 accgttgtac taagaggaaa aaaatatgga gtggagtctg acgcagaata agctgctggc
     1021 gtttcatcgc ttaatgcgta cggataagcc aattggcgcg ttactgctgc tctggccaac
     1081 attatgggcg ttgtgggtgg cgacaccggg cgttcctcag ctctggatcc tggcggtgtt
     1141 tgtcgcgggt gtctggctga tgcgcgctgc cggatgtgtg gtgaatgatt atgctgaccg
     1201 caagtttgat ggtcatgtta agcgcacggc gaaccgaccg cttcccagcg gcgcggtaac
     1261 ggagaaagag gcgcgcgcgc tgtttgtagt gctggtattg atttcatttt tactggtgct
     1321 gacgctgaat acgatgacta ttctgttgtc gattgccgcg ctggcgctgg cgtgggtata
     1381 cccatttatg aagcggtata cccatctacc gcaagtggtg ctgggcgcgg cgtttggctg
     1441 gtcgattcca atggcttttg ccgctgtgag tgagtcggtg ccattgagtt gctggttaat
     1501 gttcctcgcc aatattctct gggcggtggc ttacgacacg cagtatgcga tggttgaccg
     1561 cgatgatgat gtgaagattg gcattaaatc cacggcaatc ctgttcggcc aatacgataa
     1621 attgattatt ggtattttgc agattggcgt actggcactg atggcgatca tcggtgagtt
     1681 aaatggctta ggctggggat attactggtc aattgtggtg gctggcgcgc tgtttgttta
     1741 tcaacaaaaa ctgattgcca accgcgagcg tgaagcctgc tttaaagcat ttatgaataa
     1801 taactatgtt ggtctggtac tatttttagg gctggcaatg agttactggc atttctgatg
     1861 atgtaaaaaa gccggatgat catccggctt tcttctgggt tgcctgatgc gcggcgcttc
     1921 tcaggcctac acaacacatc gcaatttatt gaatttgcag attatggaag gccggataag
     1981 gcgttttcgc cgcatccggc aattctctct gattaccctt cgccctgcgt cgcactctca
     2041 atcgtcaaac gcacgtctga tgtaatcaac tccgccagca actgataaac cttcatcgtt
     2101 tctgccggtt cggcatcgcc gctatcgctg atatactctt catcacgcag tgtcagcacc
     2161 agagaactga acaccgcctt gtcgaagaac tccggcgcgt tgatgccgtg cagcacggag
     2221 agacgttgcg cgacggtgcg gctctctttc tccagcgtac cgcggttgat cgacgggttg
     2281 gcactcaaca accagaaggt gatggcataa cgttgcagcg tttcgcgcgc gcctgcggcg
     2341 agcagctgta gcgtgcgaga atgcaccggg ttgatatgca actcatcatc ttgcagggta
     2401 atcagcccct gacgttgcat ctcatttgcc agcgcatcaa taacgtccgg caactcgtcg
     2461 cgatcccagc gcaggaacag ctccgctttc agcattgggt aaagcacatt gacgtgctcc
     2521 atcaatacgt cgcgggagat gtggcgatgc tgggtgacaa ttgccgccat cagcgaaggc
     2581 agcaccaaca tatgcgcaat gttgttgcga tagtaggtca tcagcaccgc ttgctcgcgt
     2641 ggcagaatga tgatgtcgcc gatagtgtct ttctcgactt caaacttgtt catttgcagc
     2701 gcgtgatcga taagctcgct ggcgctggct gaaggaacgg tagagtccgt ggagtaaggt
     2761 acgttgcgca tcagatccag gtagcagttg agttgctcgg ttaactgctc gcgggtgagt
     2821 gagcgctgac gtgatgccag tagcgcagta cagcacaggt tcatggcgtt tgccgcgcct
     2881 gcgttgttaa tgcgtaccat cagatcggca gcaatattat tgaccgtcgg cgttaaccag
     2941 gccggacgca ccgcttcgat gggatcgata gattcacgcc agtctggtac gtgctggtta
     3001 aggtaggtca tcaacggcat tggttcaccg aagttgacgt aaccctgacc gagattacgc
     3061 agcttgctta aaccgcgcag catctgcggc aggctctctt tctctttcgt cgcgccgcgc
     3121 agttctttgg cgtaagtacc cacttccatg acgtgctcat aaccgatata gatcggaatc
     3181 agcgtaatcg gacgcgtgcc gccacgcagc atcgcctgaa tggtcatcga cagcgtaccg
     3241 gttttcggat ccagcaaacg ccccgtgcgg gaacgaccgc cttccacgaa gtactcgacg
     3301 gaataaccac ggctgaacag ttcgccgaga tactcacgga aaacggtgga ataaagttta
     3361 ttgcctttaa aagtacggcg aataaagaac gcccccagac ggcggaaaat cggcccggcc
     3421 ggccagaagt tcaggttgat cccggcggcg atatgcggcg gcaccagccc ctggtggtac
     3481 agcacgtaag aaagtagcag gtagtccatg tgactgcggt ggcaaggcac atacaccagc
     3541 tcatgaccgt cgtgggccag ctggcgaacg cgctcggcgt tatggacgtt gatgccctga
     3601 taaagtcggt tccaggtgaa gcccagaatg cggtcagtca ggcggatcat ctcgtaagag
     3661 aaattcgccg caatctcttc catcagcgca atcgcgttct gctgcgcttt ttcatgggag
     3721 atttttttgc tgcgcgcttc atcttctacc gctttggcaa tggcgcggga ggcgagcagc
     3781 ttattaaaca gatcctgacg agcaggcaga cgtgggccca cagcagccag acgttgacgg
     3841 gcaaagtgca tacgcgccac gcgcgccagt ttctgtgcga tagttttatc cgtgccgtgt
     3901 tcatccgcca tacggcgcag cgaaactgac ggcgagaaac gcacaaaact gtcgcgaccg
     3961 agccacagta cggcgaaaaa tttctgtacg ccgttaagca tacgcagcgg cgggtttact
     4021 tcgccttttt cacgccccgg cgcgcgacca aacatcaccg atactggcac catctgcaca
     4081 tccagatttg ggttgctacg gtgcaaatcg agatagtcgt ggaacagctt aatagactct
     4141 tctttcggcg tgtaataggt gaatacacgc ggcccgccgt gaatgaacac atagcgcggc
     4201 agtagcgtgc cgtcgatttc cagcggctct aacgggtcag gcaagtcatg agccagacac
     4261 tgggcgcgta acgtcagcaa atctgctttc gagttgtacg gtaaaacgta cataattgga
     4321 cgagaagtat ccagccccag ttccggggca ggatctgccg gaatagactt gctttttacc
     4381 aggatgctta atggtaaatt cagtaatttg tagtaaattc gtggccagcc ggacataaac
     4441 gatgtaaagc ctctggttaa taatgcaaat gcgcggcaag gatagcagaa agtcatggga
     4501 aattctgtgg tatccgctca tgtttcgcgc ggcgttacgc aaacccgaat catcggattt
     4561 aacggtacac tgatattgac gctcataatg taaaaaggtt ctttcaatgg ccaataatac
     4621 cactggattc acccgaatta tcaaagctgc tggctattcc tggaaaggtt tacgcgctgc
     4681 atggatcaac gaagcggcat tccgtcagga aggcgtagcg gtattgttgg cggtggtcat
     4741 cgcctgctgg ctggatgtgg acgcaattac ccgcgtgctg cttatcagct ccgtgatgct
     4801 ggtgatgatt gtggaaattc tcaatagcgc catcgaagca gtggttgacc gaattggctc
     4861 tgaataccat gagctttccg gacgcgctaa agatatggga tccgccgcgg tgctgattgc
     4921 cattatcgtc gccgtgatta cctggtgcat tctgttatgg tcgcattttg gataaccctt
     4981 ccagatttcg ataaatctct ggtttattgt gcagtttatg gttccaaaat cgccttttgc
     5041 tgtatatact cacagcataa ctgtatatac acccaggggg cggaatgaaa gcgttaacgg
     5101 ccaggcaaca agaggtgttt gatctcatcc gtgatcacat cagccagaca ggtatgccgc
     5161 cgacgcgtgc ggaaatcgcg cagcgtttgg ggttccgttc cccaaacgcg gctgaagaac
     5221 atctgaaggc gctggcacgc aaaggcgtta ttgaaattgt ttccggcgca tcacgcggga
     5281 ttcgtctgtt gcaggaagag gaagaagggt tgccgctggt aggtcgtgtg gctgccggtg
     5341 aaccgcttct ggcgcaacag catattgaag gtcattatca ggtcgatcct tccttgttca
     5401 agccgaatgc tgatttcctg ctgcgcgtca gcgggatgtc gatgaaagat atcggcatta
     5461 tggatggtga cttgctggca gtgcataaaa ctcaggatgt acgtaacggt caggtcgttg
     5521 tcgcacgtat tgatgacgaa gttaccgtta agcgcctgaa aaaacagggc aataaagtcg
     5581 aactgttgcc agaaaatagc gagtttaaac caattgtcgt tgaccttcgt cagcagagct
     5641 tcaccattga agggctggcg gttggggtta ttcgcaacgg cgactggctg taacatatct
     5701 ctgagaccgc gatgccgcca ggcgtcgcgg tttgtttttc atctctcttc atcaggcttg
     5761 tctgcatggc attcctcact tcatctgata aagcactctg gcatctcgcc ttacccatga
     5821 ttttctccaa tatcaccgtt ccgttgctgg gactggtcga tacggcggta attggtcatc
     5881 ttgatagccc ggtttatttg ggcggcgtgg cggttggtgc aacggcgacc agctttctct
     5941 ttatgctgtt gctgttttta cgcatgagca ccaccgggct gactgcgcag gcttatggtg
     6001 ccaaaaatcc tcaggcatta gcccgtgcgc tggtgcaacc gttgctgttg gcgttggggg
     6061 ctggggcgtt aattgcgctg ctgcgtacac cgattatcga tctggcgctg catattgttg
     6121 gcggtagtga agcggtcctg gaacaggcgc ggcgctttct tgaaatccgc tggttaagcg
     6181 ctccggcgtc gctggcgaat ctggtattac tcggttggtt actcggcgtg caatatgccc
     6241 gtgcgccagt aattttgtta gtggtcggca atatcctcaa cattgtgctg gatgtctggc
     6301 tggtgatggg gctgcatatg aacgtgcagg gcgcggcgct ggcgacggtt attgcggaat
     6361 atgcaacatt gctgattggt ctgctaatgg tgcgtaaaat cctcaaacta cgcggaattt
     6421 ccggcgaaat gctgaaaact gcctggcgag gaaacttccg tcgcttgctg gcgcttaacc
     6481 gcgatatcat gctgcgttcg ctgttgttgc aactctgttt cggcgcgatc accgtacttg
     6541 gtgcgcgact ggggagtgac attgtcgctg ttaacgcggt tctgatgacg ctactcacct
     6601 ttaccgccta tgcgcttgat ggttttgcct acgcggttga agcgcactcc ggtcaggcat
     6661 acggtgcgcg cgacggtagc cagttgctgg atgtatggcg ggcagcgtgc cgccagtcgg
     6721 ggattgtagc gttactgttt tcggtggttt atttgctggc aggggaacac atcattgcgt
     6781 tgctgacgtc gttaacccag attcagcagc tggctgaccg ctatcttatc tggcaggtga
     6841 ttttgccgtt ggttggcgtc tggtgttatc tgctggacgg catgtttata ggcgcaacgc
     6901 gtgccgccga aatgcgtaac agtatggcgg tggccgccgc aggttttgcg ctgacgctcc
     6961 ttacgctgcc gtggctgggt aatcatggtt tgtggctggc attaaccgtc tttctggcgt
     7021 tgcgcgggct ttctctggcg gctatctggc ggcgtcactg gcgcaacggt acctggtttg
     7081 ccgcaacgtg acggttaaaa attctgaata aattatccta agccaaattg ctgactacac
     7141 ttaatctcac gttcagaaga aaagtgaacg tactctcatt cacaacctaa cgatgaggtc
     7201 ttgattatga ataaagatga agccggcggt aactggaaac agtttaaagg taaagtgaaa
     7261 gagcaatggg gcaaactgac cgatgatgat atgacgatca ttgaaggtaa acgtgatcaa
     7321 ctggtcggta aaatccagga acgttatggt tatcagaaag atcaggcaga aaaagaggtc
     7381 gtggattggg aaacccgcaa tgaatatcgc tggtaattaa tccctcctgc ccgacgtgta
     7441 caaggatgta cgccctctta acgcggtttc tttttcacct gcacagagtg atcatgctgg
     7501 cactgttcag gatgacgaca cgcttccact tccacgcatg ccgcacagag cccatgtgct
     7561 tcaatcacat tatgccgcag ggcaaacccc atttttgccg ccagcgtatg cataatgtct
     7621 tccacgcctt ctgcacactc ttctttcacc gcgccgcagc gatcgcatat aaacatggct
     7681 gacgtatgcg tgggctggtc gaacagatga cagagcacat aactgttggt ggattccacc
     7741 ttatgcacaa aaccttgctc aagcagaaaa tccagcgcgc gataaaccgt tggcggcttg
     7801 gcttgcggtt cagcttcgcg cagtaaatca agcagatcat aagcgctgat agcgccatcc
     7861 tgcagactca tcaggcgcaa cacttccagg cgctgtgggg tcaggcgcac attacgctgc
     7921 gcgcagattt tttcagcctg cgctaataac tcctgcgttg tggtcttttc catcgggcac
     7981 ctcaaagtgg gggttaagaa tgtccttact ttaccatgtt ccaggaaaaa caccgagaac
     8041 ccttattgtt gccgtaatgt tgattttctg ttttgtaggt aaggtgttgt gttgccttgt
     8101 cgtaccatta tcaacacgat aataattaat aattcatttt taaataaatt gcttcaagtt
     8161 aactcgctat ttatttaact gggtttaaac gcatacctgt agttcagaaa ttatgagcga
     8221 tttttgatag tgaccagatg gcaaaagaat tataaggagg acacggtacg acaaaaaagg
     8281 tagatgaatc atgttgaaga taatacccgg tgcaactgga tactttaata aaacactcaa
     8341 ttcaaatcaa ttcgataatg aagatgcaat caaagataaa ttagataata gaggttcaat
     8401 caaaggtaaa ttaaataata tatatggtaa atctattgat tatgcagcgc tgcgtcatcg
     8461 ggatataatt attgcaaaaa tagatttgtt tattcaacga atcacgcata atttatggca
     8521 tgcacgtaaa aaaatgtgtt tttaattgag caaataaatg atctcaaaat gtgggtcaat
     8581 aagtatattg acgattgcac tgatgaggat ttaaacgatc gtgactttat tgcatcagtt
     8641 gttgaccggg ctatttttca tttcgcgatt aatagtatat gtaatcctgg ggataataaa
     8701 gatgcgactc ccattgaacg atgtactttt gatgtagaaa ctaagaatgg ccttccctcc
     8761 acggttcagc tattttatga ggaatctaag gataatgaac ctttagcgaa tatacatttt
     8821 caagcaatag gttctggttt tttaacgttt gttaatgcct gccaggaaca tgatgacaac
     8881 agcttaaaat tatttgcttc gctgttaatt tcactttcat attctagtgc ctacacagat
     8941 ttagccggag cagaaaaagt gaatattaat gaatataatg agaactatct gacagctcag
     9001 tttgaagaat tatctcaacg tgatatgaag aagtacctgg gagagatgaa gcatctggcg
     9061 gaccgggggg ggatgaaatt tgatgactat ctggataaaa tgtcacttct ggtgaatgaa
     9121 ggaaagctcg aacctgatat tttaagcaaa atgcgagatg ctgcaccgaa attaattgac
     9181 tttgctaagt cgtttgaccc aaa
//
LOCUS       DAZGVQ010000191         9054 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000191 DAZGVQ010000000
VERSION     DAZGVQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9054
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.193"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            58..330
                     /locus_tag="ACHLAG_004546"
     CDS             58..330
                     /locus_tag="ACHLAG_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFP0657659.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(331..1203)
                     /gene="rluF"
                     /locus_tag="ACHLAG_004547"
     CDS             complement(331..1203)
                     /gene="rluF"
                     /locus_tag="ACHLAG_004547"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFP0657660.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKVKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1415..2104
                     /gene="pepE"
                     /locus_tag="ACHLAG_004548"
     CDS             1415..2104
                     /gene="pepE"
                     /locus_tag="ACHLAG_004548"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFP0657661.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(2195..3826)
                     /locus_tag="ACHLAG_004549"
     CDS             complement(2195..3826)
                     /locus_tag="ACHLAG_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFP0657662.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(4046..7729)
                     /gene="metH"
                     /locus_tag="ACHLAG_004550"
     CDS             complement(4046..7729)
                     /gene="metH"
                     /locus_tag="ACHLAG_004550"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFP0657663.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            7929..8753
                     /gene="iclR"
                     /locus_tag="ACHLAG_004551"
     CDS             7929..8753
                     /gene="iclR"
                     /locus_tag="ACHLAG_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFP0657664.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         2102 a         2480 c         2323 g         2149 t
ORIGIN      
        1 tcagctcgca aacgaattcc cgtacactga actctctggc tcactgaggg caccatcatg
       61 gcactccccc gtattaccca aaaagagatg actgaacgcg agcagcgtga acttaaaaca
      121 ctgctggatc gggcgcggat tgcgcatggt cgcgtactga ccaactccga aaccaacagc
      181 attaagaaag agtacatcga taaattaatg gtcgagcgtg aggctgaagc gaaaaaagcc
      241 cgccagttga agaaaaagca ggcttataaa ccggatcctg aagcgtcatt tagctggtca
      301 gcaaatacgt cgacgcgcgg caggcgctga ttagcgcccc ttctttttac gccccggaga
      361 ggtaaaacgc ttaccgttgg acgccgggcg accgcctttt tccgccgttt tttccatctt
      421 aacgactgga cgtttgatgc ccgctgtttt cggtttggcc ttcactttag gttttgcctc
      481 ggaagaggaa ttttcgatga gcttaaagag gtcgattaac tcatcatcag ttaaatcgcg
      541 ccactcaccc agcggaatgc cgcttaaact gacgttcata atgcgcgtac gttccagctt
      601 tttcacttcg taaccaaagt gctcgcacat gcgacggatc tgacggttca gcccctgcac
      661 cagggtaatg cggaagacaa acggcgcttc ttttttgact ttacactttt tagtcactgt
      721 cccgaggatt ggcacccccg cccccatacc acgaataaat tcatcggtaa tcggtttatc
      781 gaccgtaacc agatactctt tctcatgatc gttacccgca cggaggatct tattcaccag
      841 atcgccgtga ttggtgagga aaatcagccc ctgggagtct ttatccaggc ggccaatcgg
      901 gaacacgcgt ttgctgtggt taacgaaatc gacaatgtta tcgcgctcgc catcttcggt
      961 ggtgctgaca ataccgacgg gcttgttcag ggcgataagt accaaatctt cggcttcccg
     1021 aggctcaatc aactgaccgt ttactttcac aacgtcgccg ggtttcacct gatcgccaat
     1081 ggtggctcgc ttgccattaa ggaacacatt gccttgctcg atatagcgat ccgcttcgcg
     1141 gcgtgagcaa attccgcttt cgctgatgta tttatttaaa cggactgatg agtcgggcag
     1201 cataaattct cctgtaaaaa acggaatata ccgtaccttt gggttgataa aaaatagtca
     1261 tgagcgaact atttgttatt tccattggct tgaattcgtt acctgctcaa atcactttcc
     1321 agaagcaatg tgcgacatcg tgagccatgt cagtaagtgg ttaaattcgc agtggtagca
     1381 tgccttccgt gatttcttac taaccggaga agtaatggaa ctgcttttat tgagtaactc
     1441 gacgctgccg ggtaaagcct ggctggaaca tgcactgccg ctaattgctg aacagttgca
     1501 gggtcgccgc tcagcggtgt ttatcccttt cgctggcgta acgcagacct gggatgatta
     1561 cacagcgaaa acggctgcgg ttctcgctcc gctgggtgtt tctgtcaccg gtattcatag
     1621 cgttgtcgat cccgttgccg cgattgaaaa tgctgagatc gtgattgtcg ggggcgggaa
     1681 tactttccag ttgctgaaac agtgccgcga gcgcgggctg ctggcaccaa ttactgacgt
     1741 ggttaaacgt ggtgcgctgt atattggctg gagcgcaggc gctaaccttg cttgcccaac
     1801 cattcgtacc accaacgata tgccgattgt cgatccgcaa ggtttcgatg cgctaaatct
     1861 gttcccgctg caaatcaacc cgcacttcac caacgcgctg ccggaaggcc ataaaggtga
     1921 aacccgtgag cagcgtattc gcgaactgct ggtcgtcgcg ccagaactga cgattattgg
     1981 tctaccggaa ggtaactgga tcacagtgag taaaggtcac gctacgctgg gtggcccgaa
     2041 caccacttat gtgtttaagg ctggtgaaga agcggtgccg ctagaagctg gtcaccgttt
     2101 ttaagtaaat tgccggatgc atgatccggc atcttgttca tagtgcctga tgcgacgctt
     2161 gacgcgtctt atcaggccta caggattcac aatattaata ctcgtcccgt ccttcatctt
     2221 catccggctg ttccagcaca ctgtaagcca ccgaacaaaa cagcgagttc aggcgctgca
     2281 tatctcccag taagcctaaa tgtagcgaac tggtttcaat gctctgcacg ttttgctgat
     2341 gcaggcgatc gacgtgggcg tgggaatagc ggcgattaag aatgcgaaaa cgatgtttgc
     2401 tgcgacgcaa acgacgagcg ctggtgacat cgccagagaa gaacaccgac attgccagct
     2461 ttaaattact gagcaattgc tcatagagcg catccagttc cttcaaccca tcaagcgaaa
     2521 atgcccgccg tgctgccagc gatttatcgg caatttcgct gcccatgcgc tcgacgatat
     2581 cggaagcctg ttcaaggttg agcgacattt caataatctc cgcccagcga cgcgactctt
     2641 cttctgccag ttcctctttt ggcatccgcg ccagatacag cttaatggcg gtatagagaa
     2701 cgttgatatc atctgccagc ttacgcagct ctttctcctg ccgtggctcg ccgtgcatca
     2761 ctttattcag cccttccatc atctgttcca tggcgtcgcc aatgcgcagg gtttcgcgcg
     2821 cggcgttcgc cagagcaagc gtgggcgtat ccagcgcgct gacatccaga tgtttaggcc
     2881 gtagctgggt atccagttcc ggttcatcgc gaataatcgt tttgcaaaac cgtgccatcg
     2941 ggtcaacaaa tggcagcatg accaggcaac gtacaaggtt gtagaagacg tggaaataga
     3001 tcaccagttc cgcttttggc aatgacagct tccccattgt ctctgccagc aaatggacaa
     3061 acggcaggat aatcaggcta cccaccagct taaacagcag actacccagc gcgacacggc
     3121 gggctgcggc attggcggca ctgttgttga gcatcgccag caggccggaa ccgaggttag
     3181 caccaatcac cagacagagc gccacgggga aggagataat gcctgcggcg gtcagagtcg
     3241 cagtcagcag tacagcagca aggctggagt agctgataat ggcgaacatc gcgccaatca
     3301 gcgcatccag cagaatatcg ccggtcagcg aggcaaagat cacctgaacg ccgtttgcct
     3361 gggtgatcgg cgttacggcc tgcacaatca actccagcgc cagtaaaatc agcccaagac
     3421 caataccgac gcggcccagt tgcccggcgc gtgactgttt gcgtccgagg aaaaaaatca
     3481 cgccgataaa aataagtaac ggtgacagcc aggataagtc gaaggtgaga atacgcgcca
     3541 ttagcgccgt cccgacatct gcccccagca caatgaccag agccggtgcg agggctacca
     3601 gatcctgagc gacaaacgag gtcaccagca tggtggtggc attactgctc tgcaccagtg
     3661 cggtaacgcc gatccccgcg caaaaggcga gcggcttttt ttcgacgctc cggctaagga
     3721 cagtacgcaa acgcgcgccg aagacgcgca ttacgccggt tcgaacaata tgagtccccc
     3781 agaccagcag ggcgacggca gaaagcaggt gaagcagcgt taacacgagt tcaggttctc
     3841 cttatcgtta tatgttcctg aagcaattga atgaccaaag gaccggaaac acgctgactg
     3901 gcatctttac tgtgcctttt gacattcgtt tttccggcta agattattcc agcctgtttc
     3961 cagtataagt gtttcatatt aataaagaga cagggcactc attgagcgcc ctgtttgttg
     4021 taaggaaaag tgacagattt gtgaatcagt ccgcgtcata ccccagattc ggtgccagcc
     4081 agcgctcaac atcggagacg ctcatacctt tacggcgggc atagtcttca acctgatcgc
     4141 gctgaatttg cgccaccgcg tagtacttgc tgtccgggtg gctgaaatac caacccgaaa
     4201 ccgatgcacc gggccacatg gcgaaagatt ctgtgagttt catgccggta tgtttttcca
     4261 cttccagcag ctcccagatg gtggcttttt ccgtatgttc cgggcaggcc gggtagcccg
     4321 gtgccggacg gatgccctgg tagttttcgc ggatcagttc ttcgttgctg aggttctcgt
     4381 tcggcgcata gccccagtag actttacgca cacgctcatg gagatactcc gcaaaggctt
     4441 cggctaaacg gtcggcaagc gctttcacca tgattttgtt gtaatcatcg tgctgcgctt
     4501 caaaggcatc agccagtgcg tcctcttcca gcccgccagt cacggcaaat gcgccgatgt
     4561 aatctgcttt accagaaagc ttcggcgcaa cgaagtcagc gagacagtag ttagcgaatc
     4621 ccgttttttc ggtctgttga cgcagatggt ggctgacgtt gatcacatgg gtgcgcgttt
     4681 cgtcacggta gatttcaatg tcatcgccca cacggtttgc cgggaacagg cccaccacgc
     4741 cacgcggatt cagcgttttc tcggcgctta atttatccag catgtcgttg gcgtctttaa
     4801 acagccgctg cgcctcaacg cccaccactt catcttccag aatgcgcgga tacttcccgg
     4861 ccagcgacca ggtcataaag aacggtgtcc agtcgatgta attacgcaga gtttcgatgc
     4921 tggcttcaac ttcctgcacg ccgagacggt gcgccaccgg cggcgtgtaa gcctgccagt
     4981 caaaagcgaa atcgttatcg cgcgccgctt ccagcgtgac cggtggtgtg cgcggtttct
     5041 tacgcccgtg ctgaatacgt acggtttcgt actccttgcg ggtacgagcg acaaaatcat
     5101 cacgctgagt atcggaaagc agcgccgcca ccacaccaac ggtgcgcgat gcgttctgta
     5161 catacaccgt cgggccgctg tagttctgct cgattttcac cgccgtgtgc gcttttgagg
     5221 tcgtcgcgcc gccaatcaat agcgggatgg tgaagccctg acgctccatc tctttcgcca
     5281 cgttaaccat ctcgtccagc gacggcgtga taagccccga aagtccaatc agatcagcat
     5341 tcacttcttt agcggtacgg agaatttttt ccgcaggcac cataacgccg agatcgacaa
     5401 tttcgtagtt gttacattgc agcaccacac caacgatatt tttaccgatg tcgtggacgt
     5461 cgcccttcac ggtggcgatc accatcttgc cgttggtttt gccctgctct ttgctggctt
     5521 caataaacgg ttcgagatac gccaccgcct gtttcatgac gcgcgccgat ttgaccacct
     5581 gtggcaggaa cattttccct tcgccaaaca gatcgccgac cacgttcatg ccgtccatca
     5641 acgggccttc aatcacttca atcgggcgcg tagcctgctg gcgggcttct tcggtatcct
     5701 gctcgataaa ctcggtaatg cctttgacca gcgagtattc cagacgttta ttcacttccc
     5761 acgagcgcca ctccgcctgc tgggcgttag cggtgtcgtc ggttttgctg ccgcgatatt
     5821 tctcggcaag ctccagtaaa cgctcagtgc catcatcgcg acgattaaga atcacatctt
     5881 ccaccgcgtc gcgcagttca gcgggcaggt cgtcgtaaat cgccagctgc ccggcattga
     5941 cgatccccat atccatgcca ttgcgaatag cgtagtagag gaacactgcg tgaatggctt
     6001 cgcgcaccgg atcgttgcca cggaacgaga aagaaacgtt agatacgccg ccggaaatta
     6061 gcgcgtgcgg cagttcgcgt ttgatgtctt cacacgcgcc gataaagtcc tgcgcgtagt
     6121 tgttgtgctc ttcaatgccg gtggcaaccg cgaagatgtt cgggtcgaag atgatatctt
     6181 ctggcgggaa gccaacctct tcggtgagga ttttgtacgc ccgacggcaa atctcgattt
     6241 tccgtgcgcg ggtatcggcc tgtccctgtt cgtcaaaggc cattaccacc actgccgcac
     6301 cgtaacgacg caagagcttc gcgtgatgga taaaggcgtc gacgccctct ttcatcgaga
     6361 tagagttaac aatgcctttg ccctggatac acttcagacc tttttcaatg acatcccatt
     6421 ttgaggagtc gatcataatc ggcacgcgag cgatatccgg ttcaccggca atcagattga
     6481 gaaaacgcac catcgccgct tcggcgtcga gcatcccttc atccatgttg atatcgataa
     6541 tctgcgcacc gttttccacc tgctggcggg ccacatccag tgcctcgctg tatttctctt
     6601 ctttgatcag gcgcttgaac ttagcggaac cggtgacgtt ggtgcgttca cccacgttca
     6661 caaacaggct atcttcgcca atgttcagcg gctccaggcc ggacaaacgg caggctacgg
     6721 ggatttccgg cagtttgcga ggcgctaatc cttctactgc acgactcatc gctgcaatat
     6781 gttgtggcgt ggtgccacag cagccgccga cgatattgag aaaacccgct tgcgcccatt
     6841 cacgtatctg ttttgccatc gtgtcggcgt cgagatcgta ctcaccaaag gcgttgggta
     6901 gcccggcgtt cgggtgcgcg gtgacgtagc attccgcaat ccgtgacagc tcctgcacgt
     6961 actggcgcag ttcatcgggc cccagcgcac agttcaggcc aaaggtcaga gcttcggcgt
     7021 ggcgcaatga gttgtaaaat gcttcggtgg tctgcccgga gagcgtgcgc ccggaggcgt
     7081 cggtgatggt gccggagatc ataatcggca gctcaacgcc cagcgcttca aactccgttt
     7141 tcaccgcaaa aaccgccgct ttggcgttaa gggtgtcgaa aacggtttca atcaggatca
     7201 gatccgcacc accttccacc agcgctttgg tggattcgcg ataggccgcc accagctggt
     7261 caaaagtgat gttgcggaat gccggatcgt tgacgtccgg agaaatagac gccgtgcggt
     7321 tggttgggcc gagaacaccg gcaacgtagc gcggtttctc cggcgtgcgc gcggtccact
     7381 cgtcagcaca ggcgcgcgcc agtttcgccg ccgcaaagtt gatttccgcc gacagggatt
     7441 ccatctggta atccgccatc gcaatggtcg tggagttgaa ggtgttggtt tcgatgatat
     7501 ccgcgcccgc ttcaaagtag gcgttgtgga tagcggcgat cacttccggt ttgctgagca
     7561 ccagcaggtc gttgttgcct ttgagatcgc atggccagtc ggcaaagcgt tcaccacgaa
     7621 aatcggcttc gttcagtcga tagctctgga tcatggtgcc cataccgccg tccagcacca
     7681 gaatacgttc atttaactgc gcacgcagtt gttccacttt gctgctcaca cttgctcccg
     7741 acacgctcaa cccagattta ataaaaattc aacaaaccat actggcataa acgcatctgt
     7801 ggtaaaagcg accaccacgc aacatgagat ttgttcaaca ttaactcatc ggatcagttc
     7861 agcaactatt gcattagcta acaataaaaa tgaaaatgat ttccacgata cagaaaaagg
     7921 aggctgtcat ggtcgcaccc attcccgcga aacgcggcag aaaacccgcc gttgccaccg
     7981 caccagcgac tggacaggtt cagtctttaa cgcgtggcct gaaattactg gagtggattg
     8041 ccgaatccaa tggcagtgtg gcactcacag agctggcgca acaagccggg ttacccaatt
     8101 ccacgaccca ccgcctgcta accacgatgc aacagcaggg tttcgtgcgt caggtcggcg
     8161 aactgggaca ttgggcaatc ggcgcacatg cctttatggt cggcagcagc tttctccaga
     8221 gccgtaattt gttagcgatt gttcacccta tcctgcgcaa tttaatggaa gagtctggcg
     8281 aaacggtcaa tatggcggtg cttgatcaaa gcgatcacga agcgattatt atcgaccagg
     8341 tacagtgtac gcatctgatg cgaatgtccg cgcctatcgg cggtaaattg ccgatgcacg
     8401 cttccggtgc gggtaaagcc tttttagccc aactgagcga agaacaggtg acgaagctgc
     8461 tgcaccgcaa agggttacat gcctataccc acgcaacgct ggtgtctcct gtgcatttaa
     8521 aagaagatct cgcccaaacg cgcaaacggg gttattcatt tgacgatgag gaacatgcac
     8581 tggggctacg ttgccttgca gcgtgtattt tcgatgagca ccgcgaaccg tttgccgcaa
     8641 tttcaatttc cggaccgatt tcacgtatta ccgatgaccg cgtgaccgag tttggcgcga
     8701 tggtgattaa agcggcgaag gaagtgacgc tggcgtacgg tggaatgcgc tgactttttc
     8761 tggcgggcag aggcaatatt ctgcccatca tacctgagtg gcaatagaat aagggtgtct
     8821 gttaatcgca ttgacgccaa aataacttaa tgtcatacac ttcacacact tttattaact
     8881 cagcattatt tttaaacatc aaactactta atcattaaca aaatcgcaag atctcattcc
     8941 accgaaactt ttacggctgc attatgagat tgagcgattt atgatatttc tttagttgta
     9001 atcatattaa attttttcac tgttctgata gttaaaattc aagacatcaa taaa
//
LOCUS       DAZGVQ010000192         8941 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000192 DAZGVQ010000000
VERSION     DAZGVQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.194"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..297
                     /locus_tag="ACHLAG_004552"
     CDS             <1..297
                     /locus_tag="ACHLAG_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657665.1"
                     /translation="KVRLVLDTDPEQRVNEVKNFLTALSDSICSLFIVNSNMASISLG
                     NESFSTDDDLEYGYLINAGNHYDVYLPPELFAQAYKLKNKEMNAQLDYLNRYAI"
     gene            591..1832
                     /gene="agp"
                     /locus_tag="ACHLAG_004553"
     CDS             591..1832
                     /gene="agp"
                     /locus_tag="ACHLAG_004553"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFP0657666.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAQQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDKQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(1870..2097)
                     /locus_tag="ACHLAG_004554"
     CDS             complement(1870..2097)
                     /locus_tag="ACHLAG_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFP0657667.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(2118..2723)
                     /gene="wrbA"
                     /locus_tag="ACHLAG_004555"
     CDS             complement(2118..2723)
                     /gene="wrbA"
                     /locus_tag="ACHLAG_004555"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP0657668.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVTQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            2775..4418
                     /locus_tag="ACHLAG_004556"
     CDS             2775..4418
                     /locus_tag="ACHLAG_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0657669.1"
                     /translation="MKLLATYIRWSTKEQDSGDSLRRQTNLIDAFYSKHKNDYYLLPA
                     HRYVDKGKSGFHQQHKAQGSDFRRMFENVMSGVIPEGSLIVVENFDRFSRADIDTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVKLIKHIIIAERAHQESLVKQKRIAQVWN
                     HKTQLARELKKPMGKQAPGWLELSEDGSHYIVDEDKASLVNIIYDKRLSGMSMFAICK
                     WLNEQGYPTINQRKVRISKTKKPDGNWSALSVKHILTSRSVLGYLPAKISTEDRKTVL
                     REEIEGFYPQIVTDSKFYAVQQLLEETGKGKTSSGDHWLYVNILKGLIRCKCGLVMTP
                     TGIRKPVYQGTYRCNGNKESRCSYGTVSRKLLDTQLCSRLFSKLSQLHDEATDTAKLD
                     ELQRRLNTVDSELEKLTETLIQLPNITQIQEALRVKQEEKDELIVQLSREKARVKSVS
                     SLDLSGLDMESVEGRTEAQIIIKRLVKEIVVSGNEKLVDIYLHNGNMIRGFPLDGKDD
                     HTLTLEEATDEMQPLDDMLIFGEPVTRIYPAGDMEEVDA"
     gene            complement(4438..5211)
                     /locus_tag="ACHLAG_004557"
     CDS             complement(4438..5211)
                     /locus_tag="ACHLAG_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFP0657670.1"
                     /translation="MIFSNQIRYSGKDGVYFHLRDNVDAFLTLSATENMEFDSPMQVT
                     TQNMQSGQTVTDNVQRAPRTITISGVVVVGYEGSLLLTRQGQLVENFIDTLESWRDQK
                     QIISVICKDGIKIDDSIITSFKASKDVGISNGLRVQLTFQEINFKAVVGQTDISAATG
                     KTATTNDGGATSKKNAGNVTTERNNAPMLMCQELYSYSASELSDEALKARSNCSKNVT
                     VKDGVSTFTASANEQASKVLKSGNALRKYSVNPNKKGTY"
     gene            complement(5375..7507)
                     /locus_tag="ACHLAG_004558"
     CDS             complement(5375..7507)
                     /locus_tag="ACHLAG_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFP0657671.1"
                     /translation="MSKFTVDSFIVELGFNENVIKGLQRVEKAALQSAQRIEKNLNKG
                     FRINTKQLDSNLTASLGQLERKFNKAFDKIEQRARNTKAFQFTTRFNDTVNPPKQPRQ
                     PRISGNRAITAAYSANMSKLKGLNPIVEKYIKSQFYGLSAKAGSMDSSKFNEKLAQLN
                     ASVREAIAKARGHTSTSINGDRAANSLDNLSSAAVKTAGAFISMQAVLASYQKIMEIG
                     LQRASAERSIDFVFDKDAGQVKEFTKSLAQTTGLDIAELQSQFAGFGASAKESMGIQG
                     SEELFKNMIGYARLMGRSEEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVF
                     AKATGKSEQELFDAMKKGALKSSDTLQKVTQELNKQITAKGGWKAISESTQAQLGNLK
                     NSWNTTLDSIFRGSENGLQDFTRSLTTLLNALGGSGKTLGESFGSLMSDMSHGVDNLT
                     DISYRVRAFFDEVTLAYRSLNDTQKKIVDGLSDGLIDGLKMLAVAVTAQKAANVAGGL
                     LNLANAVTSFGDAVNKAKAGKAGSSGGALSKVGRVVLYAELVNFALDNLNPEAAKNPN
                     LHQSGIGTETNFNPEYKGDSSKPNQINEGWAKIGSSIADWFNGAANKIANFQEPMSNL
                     VNNTQSTPPAITGDMVNSNAQLAMAVSELGRKVDALTAEAKKPLSVNITGEATVRPDE
                     PAFFTFNQSANDQFTQDMLLSSSYPEEE"
     gene            complement(7624..7836)
                     /locus_tag="ACHLAG_004559"
     CDS             complement(7624..7836)
                     /locus_tag="ACHLAG_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657672.1"
                     /translation="MFEERLETIRSVCERLQLQPNRQKLRIKNNRHYITSHKPKTRKI
                     PKWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(7860..8564)
                     /locus_tag="ACHLAG_004560"
     CDS             complement(7860..8564)
                     /locus_tag="ACHLAG_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657673.1"
                     /translation="MSIRNEHEMIVENRPSALDIHELSLRESFDRAWRESHEVNKGPY
                     IPPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDE
                     IDEMAILMELQTNSYAYFSRLDRDGWGYDPVEIGKVIADLPEGQRVIRTERAVRAPHR
                     LSLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKLISDHQALAKEYRQELAQII
                     SFNDFLSVPREFSKAV"
BASE COUNT         2487 a         1962 c         1874 g         2618 t
ORIGIN      
        1 aaggtacgcc ttgttttaga caccgaccca gagcagaggg ttaatgaggt gaaaaatttc
       61 ctcactgctc tttcagatag catttgctca ttatttattg ttaatagtaa tatggcatca
      121 atctccttgg ggaatgaatc cttttcaaca gatgacgacc ttgagtatgg ttatttaata
      181 aacgctggca atcattatga tgtttacctc cctcctgaac tttttgctca ggcttataag
      241 ttaaaaaata aggaaatgaa tgcgcaactc gactatttaa atcgttatgc aatttaatgg
      301 caaaggcata tgctaaaaac cattgttatt agtctcacac ttttttattg gtaaatattg
      361 tctctgtatt ggtaacgccg cagatattct gtttagccac aggtgtaatc atcagcggcg
      421 tacgcgaggc agggagctaa tcaggcatag tttgcgtcaa accttgcctg tttttaaaga
      481 tgtatataga aaaacaggca ttcaacaagc cattttgcga acctgttccc ggaaaaatgt
      541 catatttctg tcacactctt tagtgattga taacaaaaga ggtgccagga atgaacaaaa
      601 cgctaatcgc cgcaactgtg gcagggatag ttttactcgc ttcaaatgct caggcacaaa
      661 ccgtaccgga aggctatcag ctacagcaag tgctcatgat gagccgccat aacttacgtg
      721 cgccgctggc gaacaatggc agtgtgctgg agcagtctac gccgaataaa tggcccgaat
      781 gggacgtccc cggtgggcaa ctcaccacca aaggtggcgt gctcgaagtg tatatgggcc
      841 attacatgcg tgaatggctg gcacagcagg ggatggtgaa atcgggggaa tgcccgccgc
      901 cggacaccgt ttatgcctat gccaatagtc tgcaacgtac cgttgcgacc gcacagttct
      961 ttattaccgg cgcattcccg gggtgtgata ttcctgtgca tcaccaggaa aaaatgggca
     1021 ctatggaccc aacctttaac ccggtgatca ccgatgattc cgccgcattc agtgaacagg
     1081 cggtggcggc aatggagaaa gagctcagca aactccagct taccgacagc taccagctac
     1141 tggaaaaaat cgttaactat aaagattccc ctgcctgtaa agagaaacaa cagtgttcgc
     1201 tggtggatgg caaaaatacc tttagcgcca agtatcaaca agaaccaggt gtttccgggc
     1261 cgctgaaagt cggcaactcg ctggtagatg cgtttacttt gcaatattac gaaggttttc
     1321 cgatggatca ggtggcctgg ggagaaatca aatctgacaa gcagtggaag gtgttgtcga
     1381 agcttaaaaa cggctaccag gacagcctgt ttacctcacc ggaagtggcg cgcaatgttg
     1441 cgaaaccgct ggtcagttat atcgacaaag ctctggtcac cgatcgcacc agcgcaccga
     1501 aaattacagt gttggttggg cacgactcca acattgcctc tctgttaacg gcgctggatt
     1561 tcaaaccgta tcagttgcat gaccagaacg aacgcacgcc gattggcggc aaaatcgttt
     1621 tccagcgttg gcatgacagc aaagccaatc gcgatttgat gaaaattgaa tatgtgtatc
     1681 agagtgcgga acagttacgt aatgccgatg cgttaaccct gcaggcacct gctcagcgtg
     1741 tgacgctgga attaagcggt tgcccgatag atgccaacgg tttctgcccg atggataaat
     1801 ttgatagcgt gttgaatgaa gcggtgaaat aacagaaaac tcccccgcga gaagcggggg
     1861 agtcgctggt taaacgtttt tacgttcgat ggtctgttcg ccccaaaaaa gcgaatcttt
     1921 atcggtctta gcaaaggctt tgactaacac ttcatcgcta ccttcttccc aaatcttttc
     1981 cgccattttt tcgtcgtacc cggcgacttc gaaaatggcc tcggctattt ccggcgacgt
     2041 attgcgcaga gatgcccatt caccgacgtg atgagctttc gcttcttgag ttggcatgcg
     2101 tatcctcctg ttgaagatta gccgttaagt ttaactgcca gacctgcgac atattcccct
     2161 tgataacgag caatagacag ttcttcctgg cttggctggc gtgagccgtc accgcctgcg
     2221 atggtggttg cgccgtacgg cgtaccgccg cgaacctgtg taacgtcaaa taattcctgc
     2281 gctgcgtagc caatggggac aattaccatg ccgtgatgcg caagggtcgt ccaggttgaa
     2341 gtaatagttt gttcctgacc gccgccagta ccggtggaac taaagacgct cgccagtttt
     2401 ccgtatagtg cgccggaagc ccacaggccg cccgtctggt cgaggaaggt acgcatttga
     2461 ccggacatgt tgccaaagcg ggtaggtgta ccaaaaataa tggcgtcgta atcggccagt
     2521 tcttgcgggg ttgcaaccgg tgcagtttgc gttttaccgc ctgctttttc aaataattgc
     2581 ggcggcatgg tttccggtac acgcttaacg acaacttctg cgccatcgac tttgcttgca
     2641 ccctcagcga ctgcgcgtgc catcgtttca atatgtccgt acatggaata ataaagcact
     2701 agtattcttg ccttaacact catatcattg ttctcctgtt caatgaacca cttagataag
     2761 catagtccga agatatgaag ttgctagcga catacattcg ttggagtact aaagagcaag
     2821 actctggaga ctccttaaga agacaaacaa acctcattga tgctttctac tctaagcaca
     2881 aaaacgatta ttaccttctt cctgctcaca gatacgttga taagggtaaa tctggttttc
     2941 accagcaaca taaggctcaa ggttctgact tcagacgtat gtttgaaaat gttatgtctg
     3001 gggttattcc agaaggttcc ctgattgttg tagagaactt tgaccgcttc agtagggcag
     3061 atatcgacac agctatagat gacgttaggc agatactgag gaagggagtc tctatactga
     3121 ctctgggaga tggtgaactc tacgacaagt cagccttaac agaccctgtg aagttgatta
     3181 agcacatcat catagctgag agagcacatc aggaatctct tgttaagcaa aagcgtatag
     3241 ctcaggtctg gaaccataaa acacagcttg caagagaact taagaagcct atgggtaagc
     3301 aagcccccgg atggttagag ctatcagaag acggttcaca ctacatcgtt gatgaagata
     3361 aagcgtcact ggtgaatatc atctatgaca agcgtctttc tggtatgtcg atgtttgcta
     3421 tctgtaaatg gctaaacgaa caaggttacc cgaccattaa tcagcgtaag gtcagaatat
     3481 ccaagaccaa gaaaccggat ggcaactggt cagcattaag cgttaaacac atcctgactt
     3541 cacgaagtgt attaggttac cttcccgcta agatttctac agaagaccgt aaaacagtgt
     3601 tacgtgaaga aattgaaggg ttctatcctc agattgtcac agactctaag ttttacgctg
     3661 tacagcaact gttagaggaa acagggaagg ggaaaacatc cagcggtgac cactggttat
     3721 acgtgaacat actgaagggg cttatccgtt gtaagtgtgg gcttgtaatg actcctacag
     3781 ggatacgtaa acccgtttat caaggtactt accgttgcaa tggtaataag gagtcccgtt
     3841 gtagctacgg tacagtgtcc agaaagttac ttgatacaca gctttgcagt cgtctgttta
     3901 gcaaactatc acagctacac gatgaagcta cggatacagc taaattagat gagctacaac
     3961 gcagactaaa caccgttgat agtgaactgg aaaaactcac tgagacgctg atacagcttc
     4021 ctaacatcac tcagattcag gaagcactac gggttaagca ggaagaaaaa gatgaactga
     4081 tagttcagtt atcacgagaa aaggcgaggg taaaatctgt atcaagcttg gatttaagcg
     4141 gtcttgatat ggagtctgta gaggggagaa ctgaagccca gattatcatt aagcgtctgg
     4201 tgaaagagat tgttgtttct ggtaatgaaa agctggtgga tatatatctt cacaatggaa
     4261 atatgattcg tggtttccct cttgatggta aagatgacca caccttaacg cttgaagaag
     4321 caaccgatga gatgcagcca ttagatgata tgcttatctt tggtgaacca gtaacaagga
     4381 tttatcctgc tggtgatatg gaagaggtgg acgcttagga attggtattg ttaccaatca
     4441 ataagtgccc ttcttattcg gattcacaga gtactttcgt aaagcattac ccgacttaag
     4501 cacttttgaa gcctgctcat tagctgaggc tgtgaaggtg cttacaccat ctttcaccgt
     4561 gacattttta gagcaattac tacgggcttt taatgcttca tcagatagct cagacgctga
     4621 gtaactgtac agttcctgac acatcagcat aggtgcgtta ttgcgttcag tagtcacgtt
     4681 acccgcattc ttcttactgg tagcaccacc atcattcgtg gtagcggtct ttccagtagc
     4741 agcagaaata tcagtttgcc ctacaacagc tttgaagtta atttcctgaa aagtcagctg
     4801 gactcttaga ccatttgaaa taccaacatc tttagaggct ttaaaacttg taataatgga
     4861 atcatcaatt ttaataccat ctttacagat gaccgaaata atttgtttct ggtctctcca
     4921 gctttcaaga gtgtcaatga agttttctac taattgaccc tgacgagtta ataataagct
     4981 tccttcatag ccaactacaa cgacaccact aatagtaatt gttctgggtg ctctttgcac
     5041 attatctgtg acggtttgcc ctgattgcat gttctgtgta gtcacctgca tagggctgtc
     5101 aaattccatg ttttcagttg ctgataaggt taagaaggca tctacattat ctcttaagtg
     5161 gaagtaaacg ccatctttac cactgtagcg gatttgattg ctaaaaatca ttaaattctc
     5221 ctttgatttc aaagacttag tgtattttaa tcatttttaa ccaagccttt gatatataag
     5281 tgttttctct gttaagcgtg agctacgtaa aaaataattt ttggtgggta tatctgttca
     5341 gaagatttca cgtagcacac ggatatataa gaaatcattc ttcctctgga tagcttgaac
     5401 ttaagagcat atcctgcgtg aactggtcat tagctgattg attgaaagta aagaaagctg
     5461 gttcatccgg tctaacagtt gcttcaccag tgatattaac tgacaaaggc ttcttagctt
     5521 ctgctgttaa agcgtctacc tttcttccta actctgatac agccatagct aattgagcat
     5581 tactattaac catgtcacct gtgatagctg gtggagttga ttgtgtgtta ttaacaaggt
     5641 tactcattgg ctcttggaag ttagcaattt tattagctgc accattaaac cagtcagcga
     5701 tagaactacc aatcttagcc catccctcat taatttggtt tggtttagat gaatcgcctt
     5761 tgtattcagg gttgaagttt gtctctgttc caatacctga ttggtgtaag ttcggattct
     5821 tagctgcttc agggtttaaa ttatcaagag caaagtttac caactcagca taaaggacaa
     5881 ctctaccgac tttacttaat gcaccacctg aagaaccagc ctttccagct ttagctttat
     5941 taacagcgtc accgaatgaa gtaacagcgt ttgcaaggtt taacaagcct ccagctacat
     6001 ttgcagcctt ttgagcagta acagctacgg caagcatctt caagccatca attaacccat
     6061 cagataaacc atcaacaatt tttttctgag tgtcatttag tgaacgataa gccaatgtca
     6121 cctcgtcaaa gaacgccctt accctgtaac taatatcagt caggttatcg acaccatgtg
     6181 acatatcgct catcaagcta ccaaaacttt cacctaatgt tttaccactt ccaccaagag
     6241 cgtttaaaag cgttgttagg cttcttgtga aatcttgcag accattttca gaacctctga
     6301 agatactatc aagcgtggta ttccaagagt ttttcaggtt ccctagctgt gcctgtgtac
     6361 tttctgaaat agctttccag ccacctttag cggtgatttg tttattcagt tcctgagtga
     6421 ccttctgaag agtatcggaa gactttaaag cccccttctt catggcatca aataattcct
     6481 gttcactctt tccggttgct ttagcgaaca cctgcaccat accaggaaca gcttcagcca
     6541 gttgcccctt aagctcttca gccataatct gacccttacc agccatctgt gataatgctg
     6601 tcagtgctcg cttaatctct tcttcacttc tccccatcag acgagcatag ccaatcatat
     6661 ttttaaaaag ttcttcagaa ccttgaatac ccatagactc tttagctgaa gcaccaaaac
     6721 ccgcaaactg tgattgcagt tcagcgatat ctaaacccgt tgtttgagct aatgactttg
     6781 tgaactcttt tacctgacct gcatctttat caaatacgaa atcaattgaa cgttcagcac
     6841 ttgcacgctg taaaccaatt tccataatct tctgataact ggctaataca gcctgcattg
     6901 agataaatgc acctgctgtt ttaactgctg ctgaagacag gttatcaaga ctattagctg
     6961 ctctgtcacc attaatgctg gtggatgtat gacctctggc tttagcaatt gcttctctga
     7021 ctgaagcgtt taactgagct aacttctcat tgaattttga agaatccatt gacccggctt
     7081 tagcagatag cccgtaaaac tgactcttaa tgtatttctc gacaattggg ttaaggcctt
     7141 ttagcttgct catattcgct gagtaagctg ctgtgattgc tctgtttccg ctaattcgtg
     7201 gttgtcttgg ttgttttgga ggattaacag tgtcattaaa tctggtggta aactggaaag
     7261 ctttagtatt tcttgctctc tgttcaatct tgtcaaaggc cttattaaac ttcctttcaa
     7321 gctgccctaa agaagccgtt aaattactat caagctgctt tgtattaatc ctgaaacctt
     7381 tatttaggtt cttttcaatt ctttgtgctg actgcaaagc tgccttttca actctttgca
     7441 gtcccttaat aacgttttca ttgaagccta gctcaacgat aaaactatca actgtgaatt
     7501 tgctcattca tgtttcacct catagacttc ggatagaaga ctatcctcat atacaaaaaa
     7561 aaggagagtt accgatgtca ctcgataatc tctcccgttg atttttaccc aaccaattct
     7621 tttttaatgt cggacaagat aatgtaattc cgtttcacct atgatttgag catcagaagg
     7681 aatacggtca atacaccact ttggaatctt gcgtgttttt ggtttgtgtg aggtgatgta
     7741 atgacggtta ttcttaattc gtagcttttg tctatttggt tgtagctgga gtctttcgca
     7801 aacagaccga atagtctcta aacgttcttc aaacattaat cacctcacat attgcataat
     7861 caaacagcct tgctaaattc tcgtggaaca ctcagaaaat cattgaagct gataatctgt
     7921 gccaactctt gacggtactc cttagccagc gcttgatggt cactaatcag cttagccagt
     7981 cttgatttca gcaaattaat ctcattttct cttgctttca tcagtttctc tttggcaatt
     8041 tcttcaagct cagcttgtga tttatcagca acaattagag acaatcgatg tggtgctctg
     8101 acagctcttt ctgtacgaat aactctttga ccttctggta aatctgcaat cactttacca
     8161 atctctacag ggtcataccc ccaaccgtca cggtctagtc gagagaaata agcataagaa
     8221 ttggtttgaa gctccattaa aatagccatt tcgtcaattt cgtcttcaag cttggactga
     8281 attttctcgt aaacacttct cattgcttct tgagagtctt taatagcctg gaaatatcgg
     8341 cgttgtagct tcaacttcaa atcaagttca caccgttcgt tcatagaaaa atcaatttca
     8401 actcttggaa tctcgagtgg ttctggtgga atatatggac ctttatttac ttcgtgtgat
     8461 tcccgccaag ctctatcaaa agattcacgc aaacttaatt cgtgaatatc aagtgcactt
     8521 ggtctgttct caacaatcat ttcgtgttca ttacgaatag acattatttt ttcacctctt
     8581 tggtttttgt ttgtttgttt ttcaatttat agaagcacac tcagcaatca gaggtgaaga
     8641 gtctgactga acaagaacct gccaagtgtg cctttaaaaa ctgaaaaagc cctaaacata
     8701 ggtgattcgg atattcatgg aatcatcctt atctgtgaag ggctttggag gtttgcttta
     8761 taacgttaac gaagtttcag gaaaaacttt ctttaaaagc tctgtttaaa aactcttaaa
     8821 gaaaacccta atcatgagtt agggcaaagc tgtaaagggt tcaatgaatg attaagcact
     8881 ttttgagata gcctctcaga ctaaactgaa aggccatgac gaaaagcatt gcacctttcc
     8941 a
//
LOCUS       DAZGVQ010000193         8911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000193 DAZGVQ010000000
VERSION     DAZGVQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.195"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..856
                     /gene="yneK"
                     /locus_tag="ACHLAG_004561"
     CDS             <1..856
                     /gene="yneK"
                     /locus_tag="ACHLAG_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFP0657674.1"
                     /translation="KLTYPVKNGRHKENIEFQVLINLSPIYLDNFRHDGEINIFCAPN
                     PKPVTMGRVFQTGVERVSFLFLNDFIEQFPMINPGAPIKRAHTPHIEPLPPDHHTAAD
                     YLRQFDLLVLNFISRGNFVILPRLWNNSEVHRWFVNKDPNLNTAILDITDSELKEDLL
                     QSLMDSLGSNKHVLPEVCICFLSLLAEQESPHFQDLFLFFANMLLHYHQFMNPNESDL
                     NDVLMPASLSDDKIIKHMARRTLKLFVKNETPPKVTHEDLVKNRPRSPVRPPIPATSK
                     TPDLPERH"
     gene            1006..2196
                     /gene="ydeA"
                     /locus_tag="ACHLAG_004562"
     CDS             1006..2196
                     /gene="ydeA"
                     /locus_tag="ACHLAG_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFP0657675.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(2221..2886)
                     /gene="marC"
                     /locus_tag="ACHLAG_004563"
     CDS             complement(2221..2886)
                     /gene="marC"
                     /locus_tag="ACHLAG_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFP0657676.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            3098..3532
                     /gene="marR"
                     /locus_tag="ACHLAG_004564"
     CDS             3098..3532
                     /gene="marR"
                     /locus_tag="ACHLAG_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFP0657677.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            3552..3935
                     /gene="marA"
                     /locus_tag="ACHLAG_004565"
     CDS             3552..3935
                     /gene="marA"
                     /locus_tag="ACHLAG_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFP0657678.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            3967..4185
                     /gene="marB"
                     /locus_tag="ACHLAG_004566"
     CDS             3967..4185
                     /gene="marB"
                     /locus_tag="ACHLAG_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFP0657679.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4242..5681)
                     /locus_tag="ACHLAG_004567"
     CDS             complement(4242..5681)
                     /locus_tag="ACHLAG_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP0657680.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(5706..7379)
                     /locus_tag="ACHLAG_004568"
     CDS             complement(5706..7379)
                     /locus_tag="ACHLAG_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFP0657681.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     SAAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(7435..7746)
                     /locus_tag="ACHLAG_004569"
     CDS             complement(7435..7746)
                     /locus_tag="ACHLAG_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP0657682.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(7774..>8911)
                     /locus_tag="ACHLAG_004570"
     CDS             complement(7774..>8911)
                     /locus_tag="ACHLAG_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP0657683.1"
                     /translation="AGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVARNMA
                     ISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALITSEIF
                     CRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLNDLIY
                     TLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENLSWAA
                     QHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSFIPGI
                     FNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTTPGPL
                     IAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
BASE COUNT         2430 a         2136 c         1904 g         2441 t
ORIGIN      
        1 taaactcact tatcccgtca agaatggaag acacaaagaa aatattgaat ttcaggtact
       61 aataaattta agccctattt atttagataa ttttcgccat gatggagaaa ttaatatttt
      121 ttgcgctccc aacccaaagc ctgtcactat ggggcgcgta tttcagaccg gcgtcgagcg
      181 tgtgtccttt ctgtttctga atgattttat tgaacaattt ccgatgatca accctggcgc
      241 tcccatcaaa agagcgcata caccacatat tgaacccctg cctccggatc accataccgc
      301 tgcagattac ttacgccagt ttgatttgct tgtcctgaat tttatctctc gcggtaattt
      361 tgtcatactc ccccgattat ggaataactc tgaggttcac agatggttcg tcaataaaga
      421 tcctaacttg aacaccgcca ttctcgacat aacggacagc gaattaaaag aggatttatt
      481 gcaaagcctg atggattcat taggttctaa caaacatgta ctacccgaag tctgcatctg
      541 ctttttatcc cttttagcag aacaagaatc tcctcatttt caggacttgt ttttattttt
      601 cgccaatatg ttattgcact atcaccaatt tatgaatccc aatgaaagtg atttgaatga
      661 cgtgttgatg ccagcatcat taagtgatga taaaattatc aaacatatgg cgcgcaggac
      721 cctcaaactt tttgtaaaaa atgaaacgcc gccaaaagtt actcacgaag acctggtgaa
      781 aaacaggcct cgctcccctg tcagaccacc tatacccgca acctccaaaa cgccagacct
      841 ccctgaacgt cattaaaccg tgatgttacc gactctctga cgcgtgaaag aatcagcgtc
      901 agagaaacgg aaaacgcgat ccagatcaca aatgcattgt attcacatca ttaaccgttt
      961 taagatcatt tcatcacttt ttcgcaactc acccgataat ctgttatgac aacaaacact
     1021 gtttcccgca aagtggcgtg gctacgggtc gttacgctgg cagtcgccgc cttcatcttc
     1081 aacaccaccg aatttgtccc tgttggcctg ctctctgaca ttgcgcaaag ttttcacatg
     1141 caaaccgctc aggtcggcat catgttgacc atttacgcat gggtagtagc gctaatgtca
     1201 ttgcctttta tgttaatgac cagtcaggtt gaacggcgca aattactgat ctgcctgttt
     1261 gtggtgttta ttgccagcca cgtactgtcg tttttgtcgt ggagctttac cgttctggtg
     1321 atcagtcgca ttggtgtggc ttttgcacat gcgattttct ggtcgattac ggcgtctctg
     1381 gcgatccgta tggctccggc cgggaagcga gcacaggcat tgagtttaat tgccaccggt
     1441 acagcactgg cgatggtctt aggtttacct ctcgggcgca ttgtgggcca gtatttcggt
     1501 tggcgaatga ccttcttcgc gattggtatt ggggcgctta tcaccctttt gtgcctgatt
     1561 aagttacttc ccttactgcc cagtgagcat tccggttcac tgaaaagcct cccgctattg
     1621 ttccgccgcc cggcattgat gagcatttat ttgttaactg tggtggttgt caccgcccat
     1681 tacacggcat acagctatat cgagcctttt gtacaaaaca ttgcgggatt cagcgccaac
     1741 tttgccacgg cattactgtt attacttggt ggtgcgggca ttattggcag cgtgattttc
     1801 ggtaaactgg gtaatcagta tgcgtctgcg ttagtgagta cggcgattgc gctgttgctg
     1861 gtgtgcctgg cattgctgtt acctgcggcg aacagtgaaa tacacctcgg ggtgctgagt
     1921 attttctggg ggatcgcgat gatgatcatc gggcttggta tgcaggttaa agtgctggcg
     1981 ctggcaccag atgctaccga cgtcgcgatg gcgctattct ccgggatatt taatattgga
     2041 atcggggcgg gtgcgttggt aggtaatcag gtgagtttgc actggtcaat gtcgatgatt
     2101 ggttatgtgg gcgcggtgcc tgcttttgcc gcgttaattt ggtcaatcat tatatttcgc
     2161 cgctggccag tgacactcga agaacagacg caatagttga aaggcccatt cgggcctttt
     2221 ttaatggtac gttttaatga tttccaggat gccgttaata ataaactgca cacccataca
     2281 taccagcagg aatcccatca gacgggagat cgcttcaatg ccacccttgc ccaccagccg
     2341 cataattgcg ccggagctgc gtaggcttcc ccacaaaata accgccacca ggaaaaagat
     2401 cagcggcggc gcaaccatca gtacccaatc agcgaaggtt gaactctgac gcactgtgga
     2461 cgccgagcta ataatcatcg ctatggttcc cggaccagcg gtacttggca ttgctagcgg
     2521 cacaaaggca atattggcac tgggttcatc ttccagctct tccgacttgc ttttcgcctc
     2581 cggtgaatca atcgctttct gttgcggaaa gagcatccga aaaccgataa acgcgacgat
     2641 taagccgcct gcaattcgca gaccgggaat cgaaatgcca aatgtatcca tcaccagttg
     2701 cccggcgtaa tacgccacca tcatgatggc aaatacgtac accgaggcca tcaacgactg
     2761 acgattacgt tcggcactgt tcatgttgcc tgccaggcca agaaataacg cgacagttgt
     2821 taatgggtta gctaacggca gtaacaccac cagccccagg ccaattgctt taaacaaatc
     2881 taacattggt ggttgttatc ctgtgtatct gggttatcag cgaaaagtat aaggggtaaa
     2941 caaggataaa gtgtcactct ttagctaacc ttgcatcgca ttgaccaaaa cttgaaccga
     3001 tttagcaaaa cgtggcatcg gtcaattcat ttatttgact tatacttgcc tgggcaatat
     3061 tatcccctgc aactaattac ttgccagggc aactaatgtg aaaagtacca gcgatctgtt
     3121 caatgaaatt attccattgg gtcgcttaat ccatatggtt aatcagaaga aagatcgtct
     3181 gcttaacgag tatctgtctc cgctggatat taccgcggca cagtttaagg tgctctgctc
     3241 tatccgctgc gcggcgtgta ttactccggt tgaactgaaa aaagtgttgt ctgtcgacct
     3301 tggagcactg acccgtatgc tggatcgcct ggtctgtaaa ggctgggttg aaaggttgcc
     3361 gaacccgaat gataagcgcg gcgtactggt aaaacttacc accagcggcg cggcaatatg
     3421 cgaacaatgc catcaattag ttggccagga cctgcatcaa gaattaacaa aaaacctgac
     3481 ggcggacgaa gtggcaacac ttgagcattt gcttaagaaa gtcctgccgt aaacaaaaaa
     3541 gaggtatgac gatgtccaga cgcaatactg acgctattac cattcatagc attttggact
     3601 ggatcgagga caacctggaa tcgccactgt cactggagaa agtgtcagag cgttcaggtt
     3661 actctaaatg gcacctgcaa cggatgttta aaaaagaaac cggtcattca ttaggccaat
     3721 acatccgcag ccgtaagatg acggaaatcg cgcaaaagct gaaggaaagt aacgagccga
     3781 tactctatct ggcagaacga tatggcttcg agtcgcaaca aactctgacc cgaaccttca
     3841 aaaattactt tgatgttccg ccgcataaat accggatgac caatatgcag ggcgaatcgc
     3901 gctttttaca tccattaaat cattacaaca gctagttgat aacgtgacaa cgtcactgag
     3961 gcaatcatga aaccactttc atccgcaata gcagctgcgc ttattctctt ttccgcgcag
     4021 ggcgttgcgg aacaaaccac gcagccagtt gttacttctt gtgccaatgt cgtggttgtt
     4081 cccccatcgc aggaacaacc accgtttgat ttaaatcaca tgggtactgg cagtgataag
     4141 tcggatgcgc tcggcgtgcc ctattataat caacacgcta tgtagttatt gtcaggctct
     4201 gatcacagag cctgacatct ccgtaatatc ttcctcaagt ctcaaatatt ttcgccattg
     4261 ttggcgatga gatcccgata ccagtaaaac gacgctttac gtatccgttc cagcgttccc
     4321 ttcccttcgt tgtctttatc gacataaatc attccgtagc gctttttcat ttcccctgtt
     4381 ccggcagaaa ccaggtcaat gcagccccac ggggtgtagc caattaagtc aacaccatct
     4441 tcaactacgg cttttttcat ttcccgaata tgggaagcaa agtaatcaat gcgatagtga
     4501 tcgttcaccg tgccgtcagc ttgtctctgg tcaaccgcac caaatccatt ttcgacaata
     4561 aacagcggca actggaaatg atcccagaac cagttgagtg aataacgtag ccctgccgga
     4621 tcaatttgcc atccccattc ggatgtatcg atataagggt tgctgaccaa atctcgtggt
     4681 tcaacataat ccagttgcgg gttatcgggc gaaaattgtg tcgtaaaaga catgtagtag
     4741 ctaaagccga taaagtcgac acaaccactg gcaagaatcg cgttatcttc tggtgtgata
     4801 tcgaggttga atcctttcct ggcaaagtaa ttcagcatat gttgcggata atatccacga
     4861 gcatgaacat cagtaaacca gtaacgacga tgcattgctt tcgtggccat catcatatcg
     4921 ttgggtgcac acgtcagagg atagatggga cacatggcga tcatacagcc aatgttaaat
     4981 tcaggattga tcgattttcc agtctgtacc gccagggcac tggcaactaa ctcgtaatgt
     5041 accgcctggt acattatttg ttcgcgctca ttgatatctt cctctggcga atacaagata
     5101 ccggaattag taaatggaca caggctttcg ctgaaattca cctgattatt gatttcgtta
     5161 aacgtcatcc agtactttac tttttcttta tagcgcgtga agaccgttga tgcgaagcgg
     5221 ataaaaaagt cgatcagttt acggtttcgc cagccaccat attttgtcac cagatgataa
     5281 ggcatctcaa aatgtgaaag cgtcaccaca ggttccattc cctgcttaag gcattcatcg
     5341 aacagatcat cataaaattg taaaccctct tcattcggct cctgttcgtc accttgcgga
     5401 aagattcgtg tccaggcaat ggaagttcga aagcatttga atcccatctc ggcaaataac
     5461 tgaatatctg ttttatagcg atgataaaaa tcaattgctt catgattggg ataattaagc
     5521 ccgtcgataa cgccttctgt cacttcacgc ggcaccccgt gagcgccagc agtcatcaca
     5581 tcagcgatgc tgatgccttt tcctccttca ttccagccac cttccaactg atgcgcggct
     5641 accgcaccgc cccataaaaa acctttttta aatcctgaca tacacaactc cttaactaaa
     5701 atagattacc accagacttc cgcctggatg ccggccataa actcgtcttt gctgttctca
     5761 ttaaacttaa aattcgataa ttcgttatcc agaatattga tatacgttcc atagaagcga
     5821 atttctggtc gtgaacctaa aatgctttca cccactttca atgcatgcca gagtgtcgtt
     5881 ttataagccg attcatttag tgttacaccc tgctgagttt tgttctgttg cttaaaccag
     5941 cctaattcaa gccccgtctg attccatgta ttccagatcc aggccggtct tattacagtg
     6001 cgaatactgt caaaatcact atgagcacca ctttcataac tataaacatc ttcgccgtga
     6061 gaatagacaa gcgcgttagc cataataata ttgtcagtaa gatacatctc gccctgagag
     6121 attaaacgcc aggcgatccc attggtatga tcaccatagt aatagcgacc atgcgccatc
     6181 gtattactgg catctgagaa actggcaaaa ctgctggcat aggaattatt ggcaacctgt
     6241 aacgtaaatt cattaaacgt atcgcgttgc aggttttgcc gtaaaacaga agtcagcatc
     6301 cagctatctt tcatttcaaa ataactgttg tcattttcgt tatcttgttg ttgatccgtt
     6361 ttattaggtg cggaatattt agccattaat gataatgtcg catcatccca taacgggata
     6421 ttgcgataac gaacatccac agaattggta ttcatctggc tggtacgcga aaaatcacga
     6481 gagtaaacat cgacatcatc acggcttaag gacatatcaa acagccctac accaagtgcc
     6541 cagttttcaa tccccacacc cgcggcgaca tccgtggtta aggttttcca gtccagcatc
     6601 tggatctcat attgcgggag tttatgtttg ccgacccaga agtctgcctc tggtgcgaag
     6661 ggtaaaaaac ctcgcgttgt caggtaaata tcgctgaact gcatgatatt ttcattggca
     6721 ctgtcaccaa accaggcatc gttatactgc tcacccacgt tgccatcata ggtaacgacc
     6781 gcatttgccg ttttaccgtc ctgattataa acacgctgat ttaaggtcag gtcaaaccaa
     6841 ccgctcatct cattaccaaa acgtcccaga gaacccgctg cataagtttg tggtgagccg
     6901 tgattaccag taccccagcc tgagcggaag tacccctgat agctgaaccc aatatcatcc
     6961 tttatatatt tactgatatc tttcagggtc acgctttcga tagtggtttt gtcgccttta
     7021 ctttcaataa cggcaacttg ctgcgatcca tttatagtat gcgctgcggc agacgagttc
     7081 tgttcaccgt tttcattttt gatgttatca gcgacgggag atgtcgctga tatttcagca
     7141 agcgaggctg atttatattt gttttccgtg atgctttttt gtaaggtcga aagtcgggat
     7201 ttatatactc ccagttcatt ctgtgttgct ttcagctctt gtttattttg cgacaattca
     7261 ttttcaagca attcaaggcg ctgctctatg gttaattttg ttgccatcgc gttcaatgga
     7321 aacaatgctg taataataga aaaagacaaa aggctgacgt taagcgtctt tatattcatg
     7381 acttatcctt aagagtataa taatttcctc cagctatcgc actggaggaa aaaatcaggc
     7441 atggagttct tttcttaatg aaataatttc cctggctaaa tcctggcata atattgcatt
     7501 catcaaatga tcctgtgcat gaatcagaat taaattaact tttattttgc cacagccttc
     7561 atcctgacta attaatgcgg tctgaatctt atgtgcctga agacaggcag attcagaact
     7621 ggccattagc tgagttgcgc cctgccagtc tttttgcccg gcacaacgta tggcttccat
     7681 cgcgtcagaa cgtgcctgcc ccgcgttgac cagtaactcc ataacaagtt cttcatctgc
     7741 aaacatcaca atctccttcc tgggtaaatg gaattatccg ttagttgtca tgttattgac
     7801 tttgttggcg gcaataacaa aaggtaaata gatcattgtc gccacaccta aacataaaaa
     7861 accaaccagt aaagccagcc agttcccccc ggttccgagg aaagcaatta aaggtccggg
     7921 cgtagtccag ggcacggcat aagcaaccgg tggaataatt tccatcgaaa caaagaagta
     7981 accaatggca cagttaacaa tgggaaccat aataaacggc accatcatga tggggttaag
     8041 tacaatagga aggccaaaca ttatcggttc attgatattg aaaataccgg ggataaatga
     8101 catttttgcc agatcacggt attccgcacg cttagaagcg ataaaaatag ccaacaataa
     8161 ccccaacgtc atacctgaac cgccgcagtt ggcgaatgca tcattaatag aagtccaggt
     8221 aatcggatat ggcgcgcccc aggtagtgcc atgttgagcg gcccaggaga gattctccag
     8281 attagcctca gaaaaaacgg tttctcgaat tgccgatgtg gtattaggtc cgtggatccc
     8341 cagcacccac aggaaattac ccacagcccc caggataatt accgcaaaga tattcgttcc
     8401 catatgtttg agcggcgtct ggattaatgt gtaaataaga tcgtttagtc ctgccgggga
     8461 tatcagtgtc aggcaataat taagtgcgga aaagaacacc atgacaaaaa atattggcaa
     8521 taaaacttta aatgaacgcg ctaccgcagg cggtacagct gccggcatcg ttatggtgat
     8581 ttttgggttt cgagctaagc gacaaaatat ttcactggtg atcaatgcaa cgataacagc
     8641 aacaaacaac ccctgcgcgc cgagatattt ggtcgtcagg aatgcttgcc catctatcat
     8701 ctgatatggc gtataaacaa taaaaaatgc tcctattgcc gttaatccgc ataaaagatc
     8761 atcttgctca tagctaatcg ccatattcct ggcgaccagg aaagcaatca taattgacat
     8821 gatatttaca gaaccattca ttaccggagt aaaaatagct tgtgcttttt caaggttggg
     8881 gaaaatgcta ttcagatgca gtaatccggc a
//
LOCUS       DAZGVQ010000194         8902 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000194 DAZGVQ010000000
VERSION     DAZGVQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.196"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(5..1381)
                     /gene="trkA"
                     /locus_tag="ACHLAG_004571"
     CDS             complement(5..1381)
                     /gene="trkA"
                     /locus_tag="ACHLAG_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFP0657684.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1403..2692)
                     /gene="rsmB"
                     /locus_tag="ACHLAG_004572"
     CDS             complement(1403..2692)
                     /gene="rsmB"
                     /locus_tag="ACHLAG_004572"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFP0657685.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(2738..3685)
                     /gene="fmt"
                     /locus_tag="ACHLAG_004573"
     CDS             complement(2738..3685)
                     /gene="fmt"
                     /locus_tag="ACHLAG_004573"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFP0657686.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3700..4209)
                     /gene="def"
                     /locus_tag="ACHLAG_004574"
     CDS             complement(3700..4209)
                     /gene="def"
                     /locus_tag="ACHLAG_004574"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP0657687.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4339..5463
                     /gene="dprA"
                     /locus_tag="ACHLAG_004575"
     CDS             4339..5463
                     /gene="dprA"
                     /locus_tag="ACHLAG_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFP0657688.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAIVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5435..5908
                     /gene="smg"
                     /locus_tag="ACHLAG_004576"
     CDS             5435..5908
                     /gene="smg"
                     /locus_tag="ACHLAG_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFP0657689.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            5937..6479
                     /locus_tag="ACHLAG_004577"
     CDS             5937..6479
                     /locus_tag="ACHLAG_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFP0657690.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6484..7056
                     /gene="tsaC"
                     /locus_tag="ACHLAG_004578"
     CDS             6484..7056
                     /gene="tsaC"
                     /locus_tag="ACHLAG_004578"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFP0657691.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7061..7879
                     /gene="aroE"
                     /locus_tag="ACHLAG_004579"
     CDS             7061..7879
                     /gene="aroE"
                     /locus_tag="ACHLAG_004579"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFP0657692.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            7876..8133
                     /locus_tag="ACHLAG_004580"
     CDS             7876..8133
                     /locus_tag="ACHLAG_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFP0657693.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8109..8663)
                     /locus_tag="ACHLAG_004581"
     CDS             complement(8109..8663)
                     /locus_tag="ACHLAG_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP0657694.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2222 a         2179 c         2224 g         2277 t
ORIGIN      
        1 ttaattacaa gaagaaaggg cttggctgga agagtctttc gacgtcggta ataaactttt
       61 tatctgtgag gaacataatt acgtgatcgc cttgctcaat gcgcagattg tcattggcaa
      121 tcatcacgtc gtttccacgt accaccgctc caataatcgt tcctggcggt agcttgattt
      181 cgtcaatgac tctgccgaca acgcgtgagg tgctttcatc accgtgagca acggcttcaa
      241 tagcttctgc tacgccgcgg cgcaatgagg aaacaccaac aatatctgct tttcgcacat
      301 ggctaagcaa cgcagaaata gttgcttgtt gtggtgaaat cgcaatatcg atcacgctcc
      361 cctgaaccag atccacataa gcgcgacgct ggatcaatac catcaccttt ttcgcaccca
      421 tacgtttggc aagcatggcg gacatgatat tggcctcgtc atcgttggtg acggcaataa
      481 acagatcaac ttgatcgata tgttcttcgg ccagcagttc ttgatccgac gcatcaccaa
      541 aaaagacgat cgtattctgc aacttttccg ccagttcggc agcgcgctgc tgattacgtt
      601 cgatgagttt aacgctgtaa tctttttcca gacgacgcgc cagccctgca ccgatattac
      661 cgccaccaac cagcatgatc cgcttatacg gtttttccag tcgctgtaat tcactcatca
      721 ccgcacggat atgctgtgaa gcggcaataa agaacacttc atcaccagct tccacaatgg
      781 tcgaaccttg tggacgaatg gggcgatcgt ggcggaaaat tgctgccaca cgagtatcga
      841 tatgtggcat atgttcgcgc atggtcgaaa gtgcgttacc aatcagcggg ccgccataat
      901 aggctttaac cacagcgagg ctgactttac cctcagcgaa gttcaccacc tgcaatgcgc
      961 cgggatactc aatcagtcgg taaatattat cgataaccaa ctgctctggt gcgatcagat
     1021 gatcaatcgg tacagcatct gaatgaaata gcttatcggc atcgcgcacg tagtctggtg
     1081 agcggatgcg agcgatgcga ttaggggtgt tgaaaagtga gtaggctacc tggcaggcaa
     1141 ccatattggt ttcatctgaa ctggttacag caaccagcat atcggcgtcg tcggcacctg
     1201 cctcccgcaa tacgcgtgga tgagagccat gcccctgcac gactcgcagg tcaaatttat
     1261 cctgcaaggt ccgcagacgc tcaccgttgg tatcgacaac agtgatatcg ttgttctcgc
     1321 caaccaggtt ttccgccagt gtgccgccaa cctggccggc acccagaatg ataattttca
     1381 tcagtcgcga cccgttatct catcactttt tgattagctt agcgtaaaag aagccgtcgc
     1441 cctcttcggc accaggcaga ttttgtttac ccggttgctc tggtgttcct gtttcgcaaa
     1501 gttcggcatc agcggtacgt tgcaaaaagg ctttaatctg caggctattc tcttccggta
     1561 acaccgaaca ggtggcatag accagggttc caccggattt taaatgcggc caaatggcgt
     1621 cgagaatttc agactgcaat tgcgcgagtt ccgggatatc gcgatcgcga cgtaaccatt
     1681 taatatccgg atggcgacga atcacaccgg ttgctgaaca aggcgcatct aataaaatgc
     1741 gatcaaactg ttgctcgcca caccattggg aagggtagcg gccatcacct tgtttcacgg
     1801 tcgctttcat accaaggcgt tttaaattgt cgtaaacgcg agagaggcgc tgttcgtcaa
     1861 tatcaaccgc aacaacctgc gcttctggtg ccacctcaag gatatgcgtt gttttaccgc
     1921 cgggggcggc acaaagatcc agaatgtgtt caccgttttg tggcgcaagc caggtcatgc
     1981 aaccttgtgc tgatgcatcc tgaacggtaa cccatccgtc ttcaaaacca ggtagcgcat
     2041 gaacaggtgc aggtgtttcc agccgtacag catcagggta atccgcatgc gggaaacctt
     2101 tcattcctgc ttcatccaac aatgcaagcc agctgtcgcg ggaatgatgc gtacgattaa
     2161 cgcgcagcca cattggcgga cgctggttat tggcttcgac gatggattgc cactgctctg
     2221 gatacgcttt ttgcagacgc ttcagcaacc aggaaggatg cagataacgt gcatcactgg
     2281 cattaaactc ggctaataac tcttcttgct gacgctggaa ctggcgtaat acaccgttta
     2341 tcaacccttt aagttgcgga cgcttaattg cgatagcgcc ttcaaccgtt tcagccagcg
     2401 cagcatgagg tggaatgcgg gtataaagca gttgatacaa accaaccata atcaggtaat
     2461 gcacggtccg ctgtttgccg gtcatcggac gggccattaa cttattaatc agccagtcta
     2521 actgcgaaag cgtacgcagt acgccaaagc acaactcttg aagaagtgct ttgtctttat
     2581 cggaaacttt ttgctggagc ggtggcagaa tgttgcttaa tgattgccct tgctcgacga
     2641 cttgttcaac ggcctgggcc gccatgctac gtaaattacg ttgttttttc ataagtataa
     2701 aaatgcccgg caagaccggg cttagaagag tggactatca gaccagacgg ttgcccggaa
     2761 caaaccattc ccgacgagag ttcaggaggt cttgcgcgct catcgctttc ttacccgcag
     2821 gttgtaacga gagcaggttc aggatgccat caccagtcgc aacctgaatg ccttgtttat
     2881 tggcttcaag gatcgttcct ggtgcagcgt tggttgccgt atcaatgacc gacgctttcc
     2941 agactttaac cggttgtcct tcaatttcca gccagctcat tggccatggg ttgaaagcgc
     3001 gaatgcagcg ttcaagctgc gctgccgaaa gcgaccagtc aatacgcgct tcttctttac
     3061 tcaacttctc ggcgtaagtg acaagagttt cgtcctgaac ttctggtttc gccgtgccgt
     3121 ctgccagttg tttcaacgtg gtgataagcc cttgtgggcc aagctctgcc agcttgtcgt
     3181 acagcgtacc actggtatct tctgcagtaa tcgggcagga gagcttatag agcatgtcac
     3241 cggtgtctaa accaacatcc atttgcataa tggtcacacc agtttctgca tcacccgccc
     3301 atagtgagcg ttggattggt gcagcaccgc gccagcgtgg cagcagtgaa ccatgaacgt
     3361 tgatacagcc aagacgcggc atctccagca cagctttagg cagaattaaa ccataggcga
     3421 cgacgaccat aacatcagcc tgcagatcgg cgaccagttg ctggttttct tgtggacgca
     3481 gggaaacagg ttgaaaaacg ggcagacctt tttcctcagc cagaacttta atcgggctgg
     3541 gcatcagttt tttaccgcgt cctgccggtc ggtctggctg ggtgaacacg ccaacgacgt
     3601 tatgaccaga agacaacagc gcgtcgagat gacgcgctgc aaagtcaggt gtacccgcaa
     3661 aaataatacg tagtgattct gacacgttag ttcttatcct taagcccggg ctttcagacg
     3721 atccagtttt tcaactttct gacgaatacg ttgttgtttc agcggtgaca gataatccat
     3781 aaacagtttg ccgaccaggt gatccatctc atgctgaata cagatggcta acagaccatc
     3841 tgcctccagt tcaaatggtt taccgtcgcg gtccagggcg cgaattttaa ctttctctgc
     3901 gcgtggcact aaagcacgtt gttcagggat cgacaggcaa ccttcttcaa tgcctgtttc
     3961 gccgcttttt tctaaaagct ccggattgat taacaccagc cgttcgtcac ggttttccga
     4021 aacatcaata acaatgatac gttgatggat atcaacctgg gttgccgcca ggccaatacc
     4081 ttcttctgca tacatcgtct cgaacatatc atcgacgata cgctgaattt ctgcattcac
     4141 ttcttctacc ggtttagcaa ctttgcgaag ccgctcgtcc ggaatatgta acacttgcaa
     4201 aactgacata aatctccaga gatgtgttca ggagttagaa agattatttc ttctattcta
     4261 gacaaatccc cctctgattg acagcatcac tgaccaatcg caaagattgc taaggctgct
     4321 tatggcaggg agataaggat ggtcgataca gaaatttggc tgcgtttaat gagtatcagc
     4381 agcttgtacg gcgatgatat ggtccgtata gctcactggc tggcaaaaca gtcgcatatt
     4441 gatgcggttg tattgcagca aactgggctt acattgcggc aggcacaacg ctttctttca
     4501 tttccgcgaa agagtatcga aagctcactt tgttggttgg agcaacccaa ccatcattta
     4561 atccctgcgg acagcgaatt ttatcctcct caacttcagg cgacgacaga ttaccccggc
     4621 gcactgtttg ttgaaggaga actgcacgcg ctgcattcat ttcagcttgc catagtgggg
     4681 agtcgggcgc attcatggta tggcgagcga tggggacgat tattttgcga aactctggcg
     4741 acgcgtggag tgacaattac aagtggactg gcgcgtggaa tcgatggtgt ggcgcataaa
     4801 gcggccttac aggtaaatgg cgtcagcatt gctgtattgg ggaatggact taataccatt
     4861 catccccgcc gccatgcccg actggctgcc agtttgcttg aacatggcgg agctctcgtc
     4921 tcggaatttc ccctcgatgt tccacccctt gcttacaatt tcccacgaag aaatcgcatt
     4981 atcagtggtc taagtaaagg tgtactggtg gtggaagcgg ctttgcgcag tggttcgcta
     5041 gtgacggcac gttgtgcgct tgagcagggg cgtgaagttt ttgccttgcc aggtccaata
     5101 gggaatccgg gaagcgaagg gcctcactgg ttaataaaac aaggtgcgat tcttgtgacg
     5161 gaaccggaag aaattctgga aaacttgcaa tttggattgc actggttgcc agacgcccct
     5221 gaaaattcat tttattcacc agatcagcaa gacgtggcat tgccatttcc tgagctcctg
     5281 gctaacgtag gagatgaggt aacacctgtt gacgtcgtcg ctgaacgtgc cggccaacct
     5341 gtgccagagg tagttactca actactcgaa ctggagttag caggatggat cgcagctgta
     5401 cccggcggct atgtccgatt gaggagggca tgccatgttc gacgtactaa tgtatttgtt
     5461 tgaaacctat attcacacag aagctgagtt gcgtgtggat caagacaaac ttgaacagga
     5521 tcttaccgac gcaggatttg atcgagaaga tatctacaat gccctgcttt ggctggaaaa
     5581 gcttgcggat tatcaggaag gactggcaga accgatgcaa ctggcctctg atccactctc
     5641 catgcgtatt tatacccctg aggagtgtga aagactggat gccagctgtc gtggttttct
     5701 gcttttcctt gagcagattc aggtgctcaa ccttgaaact cgtgaaatgg tgatagagcg
     5761 agtgctcgcg ctggataacg cagagttcga actggatgat ctgaaatggg taatcctgat
     5821 ggtgttgttc aatattccgg gctgcgaaaa tgcgtaccag caaatggaag aattactctt
     5881 tgaagtgaat gaaggtatgc tgcattaatc atttctttaa ttcagcataa gttgttatgg
     5941 cgaaatcagc actgttcacg gtgcgtaata atgagtcctg cccaaagtgc ggggctgaac
     6001 tggttattcg atccgggaaa cacggtccgt ttcttggatg ctcacagtat ccggcgtgtg
     6061 actacgtccg tcctctgaaa tcttcagcgg atggacatat tgtcaaagtt ctggaggggc
     6121 aggtttgccc tgcatgtggc gcaaatctgg tattacgcca gggacgcttt ggtatgttta
     6181 ttggttgcag taactaccct gaatgcgaac ataccgaact tatcgataaa ccggacgaaa
     6241 cagcaattac atgcccccaa tgccggacgg gccatctggt ccagcgccgc tcccgttatg
     6301 gcaaaacatt tcactcctgt gatcgctacc cggagtgtca atttgccatt aacttcaaac
     6361 ccatagctgg agaatgccct gagtgtcatt atccactact catcgaaaag aaaaccgcgc
     6421 agggtgtaaa acacttttgt gccagtaaac aatgtggaaa gccggtttcg gcggaataat
     6481 aacgtgaata ataacctgca aggagacgct atcgcagctg cgatagatgt cctcaatgaa
     6541 gaacgtgtca tcgcctatcc aacggaagcc gttttcggtg tcgggtgcga tcctgatagc
     6601 gaaacagcag tgatgcgact gttggagtta aaacagcgtc cggttgataa ggggctgatt
     6661 ttaatcgcgg caaattacga gcagcttaaa ccctatattg atgacaccat gctgactgat
     6721 gcgcagcgtg aaaccatttt ttcccgctgg ccaggtcctg tcacctttgt ctttcccgcg
     6781 cctgcgacaa caccgcgctg gttgacgggc cgctttgatt cgcttgctgt acgagtcacc
     6841 gaccatccgt tggtggttgc tttgtgccag gcttatggta aaccgctggt ttctaccagt
     6901 gccaacttga gtggattgcc accttgtcga acagtagacg aagttcgcgc acaatttggc
     6961 gcggcgttcc cggttgtgcc tggtgaaacg ggggggcgtt taaatccttc agaaatccgc
     7021 gatgccctga cgggtgaact gtttcgacag gggtaacata atggaaacct atgctgtttt
     7081 tggtaatccg atagcccaca gcaaatcgcc attcattcat cagcaatttg ctcaacaact
     7141 gaatattgaa catccctatg ggcgcgtgtt ggcacccatc aatgatttca tcaacacact
     7201 gaacgctttc tttagtgctg gtggtaaagg tgcgaatgtg acggtacctt ttaaagaaga
     7261 ggcttttgcc agagcggatg agcttactga acgggcagcg ttggctggtg ctgttaatac
     7321 cctcaagcgg ttagaagatg gacgcttgca gggtgacaat accgatggta taggtttgtt
     7381 aagcgatctg gaacgtctgt cttttatccg tccgggttta cgtattctgc ttatcggcgc
     7441 tggtggagca tctcgcggcg tactactacc actcctttcc ctggactgtg cggtgacaat
     7501 cactaatcgg acggtatccc gcgcggaaga gttggctaaa ttgtttgcgc acactggcag
     7561 tattcaggcg ttgggtatgg acgaactgga aggtcatgag tttgatctca ttattaatgc
     7621 aacatccagt ggcatcagtg gtgatattcc ggcgatcccg tcatctctca ttcatccagg
     7681 catttattgc tatgacatgt tctatcagaa aggaaaaact ccttttctgg catggtgtga
     7741 gcagcgaggc tcaaagcgta atgctgatgg tttaggaatg ctggtggcac aggcggctca
     7801 tgcctttctt ctctggcacg gtgttctgcc tgacgtagaa ccagttataa agcaattgca
     7861 ggaggaattg tccgcgtgaa tcaggccatc cagtttccgg acagagaaga gtgggacgag
     7921 aataaaaaat gtgtatgttt tcccgctctc gtgaatggca tgcaactgac atgtgcgatc
     7981 tctggcgaga gtctggcgta tcgttttact ggagatacgc cagaacagtg gttagcgagt
     8041 tttcgtcagc atcgctggga tctggaagaa gaagcggaaa acttaattca ggaacaaagt
     8101 gaagatgatc aaggctgggt ctggttaccc tgatccagat attcgtcctt ccatttcacg
     8161 taattattcg cggaatagcg taacccagcc ttctcttcat cacttaacgg gcggatctgt
     8221 ttgacggggc taccgagata cagatatccg ctctccagcc gtttattttg tgggaccaga
     8281 ctacccgcac caatcatcac atcatcttct actattgcgc catcaagtaa aattgagccc
     8341 atcccaacca aaactcgatt gccaatggtg cagccgtgga gcatcacctt gtgaccaaca
     8401 gtgacatctt cgccaatggt taatgggttg ccatctgggt tgtacgagga tttatgagtg
     8461 acatgcaaca tactgccatc ctggatattg gtgcgtgctc cgatctgtac ataatgtaca
     8521 tctccacgaa tcacaacgag cggccagatc cccacatcat cagccagacg aacgtcacca
     8581 atcacgacac tgctatcgtc gatcattacg cgctgaccga tttgtggaaa aagatcgcgg
     8641 tatgggcgta aaacatcaga catacttacc tcagcaataa atgatttact aatgactttg
     8701 ggggcattat tggccttgtg caagtctttt agtatgcaaa aaagcaccgt tttgtgtgcg
     8761 attgcagcaa aaagggtgaa aaaacaacaa acagaaaaaa agatcaaaaa aatacttgtg
     8821 caaaaaattg ggaaccctat aatgcgcctc cgttgagacg acaacgtgaa acacttcaca
     8881 ggatggtcgg aacaacgaag ag
//
LOCUS       DAZGVQ010000195         8886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000195 DAZGVQ010000000
VERSION     DAZGVQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.197"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(129..3491)
                     /gene="mscK"
                     /locus_tag="ACHLAG_004582"
     CDS             complement(129..3491)
                     /gene="mscK"
                     /locus_tag="ACHLAG_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFP0657695.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(3619..4266)
                     /gene="acrR"
                     /locus_tag="ACHLAG_004583"
     CDS             complement(3619..4266)
                     /gene="acrR"
                     /locus_tag="ACHLAG_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFP0657696.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            4408..5601
                     /gene="acrA"
                     /locus_tag="ACHLAG_004584"
     CDS             4408..5601
                     /gene="acrA"
                     /locus_tag="ACHLAG_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFP0657697.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            5624..8773
                     /gene="acrB"
                     /locus_tag="ACHLAG_004585"
     CDS             5624..8773
                     /gene="acrB"
                     /locus_tag="ACHLAG_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFP0657698.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
BASE COUNT         2135 a         2280 c         2323 g         2148 t
ORIGIN      
        1 cagctcctgc aatacattga attcttgcgg tttcgtaggc cggataaggc ggtcacgccg
       61 catccggcat gaacaacgcg cactttgtca gtaatctcgg ggcaactaaa tgtcgccacc
      121 tttggttatt accctaccgc tggcgtcggg tcatcgcctt tgtagtcgcg tttgacttcc
      181 gtcacctcat cgcccttctc gttgtgcaga tgcacttcaa gctggttaaa ggcaatgttg
      241 atgtcgtttt cacggcacag ctgatcgata gtacggttca gctcatcgac agtacgacta
      301 cggtcacgca gttcacgcac atacagacgc agctcatgat ccaacgtgct ggcaccaaac
      361 gccgtaaaga agacttccgg cattggttcg tgcatcaccc ttgggtgctc agtcgccgcc
      421 ttcagtaaca ctttacgcac tttttccaga tcggagccat aggccacgcc gagacggatc
      481 accagacgcg tagtagtgtc agtcaacgac cagttgatca gacgctcggt aacaaacgct
      541 ttgttcggga tgatcacttc tttgcgatcg aaatcggtaa tcgttgtcgc acgaatacgg
      601 atcttactta ccgtccccga gaagctacca atggttaccg tatcgccaat acgcaccgga
      661 cgttcgaaca gaatgatcaa accggagacg aagttaccga aaatttcttg taaaccaaaa
      721 ccaagaccta cggataatgc tgcggccagc cactggagtt tatcccaaga gacgcccagc
      781 gatccgaaca ccgtcatcgc accaacggca ataatgatgt agttaaggat ggtagtaatg
      841 gcatacgacg cgccctgacg catattcagt cgcgagagca ccagcacttc cagtaaacca
      901 ggcaggttgc gaatcaacgc ccaggccacc attgaggcga taatcgcaaa caacagactg
      961 cccatggtga cgtttttcac caccgcagcg ccagcttcag tgccgttgta atgccagagc
     1021 gtgatgctgt cgagatagct gaacacggtg atcaaatcgg accaaattgc ccagaacatg
     1081 acaccgaaca gcgcaaacat cagcaacatg gtaatacgca gcgtctgctg gttaacttgc
     1141 tccagtgcaa tggtgggttc ttccggcggt tcggcacctt ctgcgccctc tttcaccaga
     1201 ttctgccgac gcgccagcgc acgacgccag gcgatacgcc gcgccgctac gcttaagccg
     1261 cgcaataccg tctggtacag caggttccag atgatcacca aataaacggt ttcaatccaa
     1321 cgcccggaga ggcgcagcgt ggtgtagaaa taacccgtgg cagtcagcac catcaacgca
     1381 atcgggataa tcgatagcac ggtaatggtg accagtcgca tggtgtgcga ctctttatca
     1441 cgccagcttt cgcggcacat tggccatacc aggaaggcaa tcagcagcag gttgaagaaa
     1501 atcatcgcct gccccagcac atcatccatc agatgcagcg gggaaagttc tgctaccaca
     1561 gaccagaaat ggataggcag caacgcgaga ctgatgcgga caatttgccg acgccagtgg
     1621 ctggtctgct gctccggcat gccgaagtga cgcacggcaa cgccattttt ctccagtacc
     1681 ttccagcaca ggccaaacac cagccagaaa atcgccagtt ttttactgaa cgaccatagc
     1741 agttcgctga tgttgagctg catggtcaac agaatcaggc caaccgcgag aataatcagg
     1801 cacaccggca gcgcgcggat caggtcgata aggatcgctt ttggtgtgtt gagctggcta
     1861 tcgttacgca gggaacccac agcggaagcc agtttctgtt gatacgcttt cagccagccc
     1921 agacgccagt ggatcagccc ggcaatcaac agcagcggca aaccggcgag gaaagcgata
     1981 aaaacggcag gccaggcttt ttcccagttc accgtgattt tcatcgactt aaattcatct
     2041 ttcaggcttt gcgggaacgc tttgatccag tcccagtcca ttggacggtt actgttcacc
     2101 caaaagattt gctgagtcag gatggatttc aggtttttcg acacactcat taactgctgc
     2161 tggttgattt gcaggttaat ggccatcatc agctggttac ccaactgttt gttgagttga
     2221 tccagcaatt cgcgacgcat atcaaccact tgcaataacg catcgtgaac ttcgctgttg
     2281 acttcgttgg tgtgaccttc ttccagtttg ttgacgaacg catcgctctg gaaaagtgca
     2341 tcacgctgct ggttaacttc aaactgttcg agacgcaaat ccgcgatgcg gttggtcatg
     2401 ttttccagtt catccgccga gggcagcgtt tgttgttgct ggtaaaggat acgagacaac
     2461 agcaggctgc ccttcaggac ggcaatctgc tctttaatat tgcgttccga ttgcagcgcc
     2521 cgctccagcc agtttttgac tttaatgttt tgctgcatca actgattacc gttttcagtc
     2581 gcggtaatca gacgctgact taactgctgg ttaatttcca gttcctgttt caccagcgga
     2641 ttagcctgaa tacgcgcggc ttcatccggg gagacggctt cctgcgccgt tttttcagtt
     2701 aaagtcaggc gcttgctgtt taccgcttct tgcaacagct gcaactggtg ctccagacgg
     2761 gcgctgttcg ccgtcacgta atcacgttgc ttttgcaagg tatcctgcaa gacggtgttc
     2821 ccttccaggc ttttacgctg ctggtcaatc tccgcattca gcaacgcctg ctgagcctgc
     2881 attaacactt tctggctggg acgtaaggca gtctcgccga catcagtccc atccagacga
     2941 ctgcgaattt gttgcagctg ctgcgaagcg ttatacatcg cattttgcac gcgttcgggc
     3001 tgcgtctgta acgaaaccag ctggctgtta taagacgcca gatcgttttg tgcgttttgc
     3061 aaatcgtcca gcgcctgggc aacgcgagtt tccagctggc gcaacgaaag cgtactcaga
     3121 attttgcgcg tttcttcgtc gttatcgaca tcgctaagtg ctgttaacgc cgcggtcgcc
     3181 tggcgcattt tttccggcgc ttcagcgact ttttgccgta gctgaactgt ctcttctttt
     3241 atgcgatcga ttttatcgag ggtggctaat gtatctgtca gatcctgctg caccagtttg
     3301 tcctgagcag aaagatcttt ttgtttattt agtgagtcaa gttgcgcctg caggtccgct
     3361 tttgtcggca gatcaccatt cgatgacgcc cgcgcaaacg ccgtgttctg gcataacaag
     3421 acaaaaacaa aagcaataaa tgctgaaaaa acaaaatgcc gtgatcgttt gtaatactgg
     3481 aacatagtca tgatgaatga aggtttctga acctgaagaa cgacctgaaa aagtcaaacc
     3541 gcaagaatat cacgacgcag tgaaccagaa tagcaacgac gaaaatgtcc aggaaaaatc
     3601 ctggagtcag attcagggtt attcgttagt ggcaggatta cgaagcgtgg ggcacaggag
     3661 atacatctcc agtaagatgg cgacgtaatc gcgggcttct tttttaagat caaaagattg
     3721 cggggcaaag agccagtttt ccatcaggcc ggaaatatag ccgcgcataa taattgctgc
     3781 ccgacgcgtc attaaatccg caggcaacat tttcgcttca atacaatgtt ttaacgtttg
     3841 ttctatacgg tcataacttt ccagacagag attacgttgt gcctgttgca caacagccat
     3901 ttctccgaca aattcgcatt tgtggaatat aatctccatc aataatcgac gtcgttcttc
     3961 tgtcaccgtg gattcaagaa catgaattaa tatctctctt aatactgaga gtggatcgcc
     4021 agggaatttt gcctgatact caagctctag ttcaccaata ttggattctg acagttccca
     4081 gatctcactg aacaaatccg acttgtcttt aaaatgccag tagattgcac cgcgcgtaac
     4141 gccagctgct tttgcaatct cgcccagcga ggtggatgat accccctgct gtgagaaaag
     4201 acgtagagcc acatcgagga tgtgttggcg cgtttcttgc gcttcttgtt tggtttttcg
     4261 tgccatatgt tcgtgaattt acaggcgtta gatttacata catttgtgaa tgtatgtacc
     4321 atagcacgac gataatataa acgcagcaat gggtttatta acttttgacc attgaccaat
     4381 ttgaaatcgg acactcgagg tttacatatg aacaaaaaca gagggtttac gcctctggcg
     4441 gtcgttctga tgctctcagg cagcttagcc ctaacaggat gtgacgacaa acaggcccaa
     4501 caaggtggcc agcagatgcc cgccgttggc gtagtaacag tcaaaactga acctctgcag
     4561 atcacaaccg agcttccggg tcgcaccagt gcctaccgga tcgcagaagt tcgtcctcaa
     4621 gttagcggga ttatcctgaa gcgtaatttc aaagaaggta gcgacatcga agcaggtgtc
     4681 tctctctatc agattgatcc tgcgacctat caggcgacat acgacagtgc gaaaggtgat
     4741 ctggcgaaag cccaggctgc agccaatatc gcgcaattga cggtgaatcg ttatcagaaa
     4801 ctgctcggta ctcagtacat cagtaagcaa gagtacgatc aggctctggc tgatgcgcaa
     4861 caggcgaatg ctgcggtaac tgcggcgaaa gctgccgttg aaactgcgcg gatcaatctg
     4921 gcttacacca aagtcacctc tccgattagc ggtcgcattg gtaagtcgaa cgtgacggaa
     4981 ggcgcattgg tacagaacgg tcaggcgact gcgctggcaa ccgtgcagca acttgatccg
     5041 atctacgttg atgtgaccca gtccagcaac gacttcctgc gcctgaaaca ggaactggcg
     5101 aatggcacgc tgaaacaaga gaacggcaaa gccaaagtgt cgctgatcac cagtgacggc
     5161 attaagttcc cgcaggacgg tacgctggaa ttctctgacg ttaccgttga tcagaccact
     5221 gggtctatca ccctacgcgc tatcttcccg aacccggatc acactctgct gccgggtatg
     5281 ttcgtgcgcg cacgtcttga agaagggctt aatccaaacg ctattttagt cccgcaacag
     5341 ggcgtaaccc gtacgccgcg tggcgatgcc accgtactgg tagttggcgc ggatgacaaa
     5401 gtggagaccc gtccgatcgt tgcaagccag gctattggcg ataagtggct ggtgacagaa
     5461 ggtctgaaag caggcgatcg cgtagtaata agtgggctgc agaaagtgcg tcctggtgtc
     5521 caggtaaaag cacaagaagt taccgctgat aataaccagc aagccgcaag cggtgctcag
     5581 cctgaacagt ccaagtctta acttaaacag gagccgttaa gacatgccta atttctttat
     5641 cgatcgcccg atttttgcgt gggtgatcgc cattatcatc atgttggcag gggggctggc
     5701 gatcctcaaa ctgccggtgg cgcaatatcc tacgattgca ccgccggcag taacgatctc
     5761 cgcctcctac cccggcgctg atgcgaaaac agtgcaggac acggtgacac aggttatcga
     5821 acagaatatg aacggtatcg ataacctgat gtacatgtcc tctaacagtg actccacggg
     5881 taccgtgcag atcaccctga cctttgagtc tggtactgat gcggatatcg cgcaggttca
     5941 ggtgcagaac aaactgcagc tggcgatgcc gttgctgccg caagaagttc agcagcaagg
     6001 ggtgagcgtt gagaaatcat ccagcagctt cctgatggtt gtcggcgtta tcaacaccga
     6061 tggcaccatg acgcaggagg atatctctga ctacgtagcg gcgaatatga aagatgccat
     6121 cagccgtacg tcgggcgtgg gtgatgttca gttgttcggt tcacagtacg cgatgcgtat
     6181 ctggatgaac ccgaatgagc tgaacaaatt ccagctaacg ccggttgatg tcattaccgc
     6241 catcaaagcg cagaacgccc aggttgcggc gggtcagctc ggtggtacgc cgccggtgaa
     6301 aggccaacag cttaacgcct ctattattgc tcagacgcgt ctgacctcta ctgaagagtt
     6361 cggcaaaatc ctgctgaaag tgaatcagga tggttcccgc gtactgctgc gtgatgtggc
     6421 gaagattgag ctgggtggtg agaactacga catcatcgca gagtttaacg gccaaccggc
     6481 ttccggtctg gggatcaagc tggcgaccgg tgcaaacgcg ctggataccg ctgcggcaat
     6541 ccgtgctgaa ctggcgaaga tggaaccgtt cttcccgtca ggtctgaaaa ttgtttaccc
     6601 gtacgacacc acgccgttcg tgaaaatctc tattcacgaa gtggttaaaa cgctggtcga
     6661 agcgatcatc ctcgtgttcc tggtgatgta tctgttcctg cagaacttcc gcgcgacgtt
     6721 gattccgacc attgccgtac cggtggtatt gctcgggacc tttgccgtcc ttgccgcctt
     6781 tggcttctcg ataaacacgc taacaatgtt cgggatggtg ctcgccatcg gcttgttggt
     6841 ggatgacgct atcgttgtgg tagaaaacgt tgagcgtgtt atggcggaag aaggtttgcc
     6901 gccaaaagaa gctacccgta agtcgatggg gcagattcag ggcgctctgg tcggtatcgc
     6961 gatggtactg tcggcggtat tcgtaccgat ggccttcttt ggcggttcta ctggtgctat
     7021 ctatcgtcag ttctctatta ccattgtttc agcaatggcg ctgtcggtac tggtagcgtt
     7081 gatcctgact ccggctcttt gtgccaccat gctgaaaccg attgccaaag gcgatcacgg
     7141 ggaaggtaaa aaaggcttct tcggctggtt taaccgcatg ttcgagaaga gcacgcacca
     7201 ctacaccgac agcgtaggcg gtattctgcg cagtacgggg cgttacctgg tgctgtatct
     7261 gatcatcgtg gtcggcatgg cctatctgtt cgtgcgtctg ccaagctcct tcttgccaga
     7321 tgaggaccag ggcgtattta tgaccatggt tcagctgcca gcaggtgcaa cgcaggaacg
     7381 tacgcagaaa gtgctcaatg aggtaacgca ttactatctg accaaagaaa agaacaacgt
     7441 tgagtcggtg ttcgccgtta acggcttcgg ctttgcggga cgtggtcaga ataccggtat
     7501 tgcgttcgtt tccttgaagg actgggccga tcgtccgggc gaagaaaaca aagttgaagc
     7561 gattactatg cgtgcaacac gcgctttctc gcaaatcaaa gatgcgatgg ttttcgcctt
     7621 taacctgccc gcaatcgtgg aactgggtac tgcaaccggc tttgactttg agctgattga
     7681 ccaggctggc cttggtcacg aaaaactgac tcaggcgcgt aaccagctgc ttgcagaagc
     7741 agcgaagcac cctgatatgt tgaccagcgt acgtccaaac ggtctggaag ataccccgca
     7801 gtttaagatt gatatcgacc aggaaaaagc gcaggcgctg ggtgtttcta tcaacgacat
     7861 taacaccact ctgggcgctg catggggtgg tagctatgtg aacgacttta tcgaccgcgg
     7921 tcgtgtgaag aaagtttacg tcatgtcaga agcgaaatac cgtatgctgc cggatgatat
     7981 cggcgactgg tatgttcgtg ctgctgatgg tcagatggtg ccgttctcgg cgttctcctc
     8041 ttctcgttgg gagtacggtt cgccgcgtct ggaacgttac aacggcctgc catctatgga
     8101 aatcttaggc caggcggcac cgggtaaaag taccggtgaa gcaatggagc taatggaaca
     8161 actggcgagc aaactgccta ccggtgtggg ctatgactgg acggggatgt cctatcagga
     8221 acgtctctcc ggcaaccagg caccttcact gtacgcgatt tcgttgattg tcgtgttcct
     8281 gtgtctggca gcactgtacg agagctggtc gattccgttc tccgttatgc tggtcgttcc
     8341 gctgggggtt atcggtgcgt tgctggctgc caccttccgt ggcctgacca atgacgttta
     8401 cttccaggta ggcctgctca caaccattgg gttgtcggcg aagaacgcga tacttatcgt
     8461 cgaattcgcc aaagacttga tggataaaga aggtaaaggt ctgattgaag cgacgcttga
     8521 tgcggtgcgg atgcgtttac gtccgatcct gatgacctcg ctggcgttta tcctcggcgt
     8581 tatgccgctg gttatcagta ctggtgctgg ttccggcgcg cagaacgcag taggtaccgg
     8641 tgtaatgggc gggatggtga ccgcaacggt actggcaatc ttcttcgttc cggtattctt
     8701 tgtggtggtt cgccgccgct ttagccgcaa gaatgaagat atcgagcaca gccatactgt
     8761 cgatcatcat tgatacaaca tgtaatcact aaggccgcgt aagcggcctt ttttatgcat
     8821 aacctacgaa cattaaggag taattgaacc accaactcag gatctcatac gaaaaccagt
     8881 attaac
//
LOCUS       DAZGVQ010000196         8536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000196 DAZGVQ010000000
VERSION     DAZGVQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.198"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(231..437)
                     /locus_tag="ACHLAG_004586"
     CDS             complement(231..437)
                     /locus_tag="ACHLAG_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFP0657699.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            750..839
                     /gene="kdpF"
                     /locus_tag="ACHLAG_004587"
     CDS             750..839
                     /gene="kdpF"
                     /locus_tag="ACHLAG_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFP0657700.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            839..2512
                     /gene="kdpA"
                     /locus_tag="ACHLAG_004588"
     CDS             839..2512
                     /gene="kdpA"
                     /locus_tag="ACHLAG_004588"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFP0657701.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2535..4583
                     /gene="kdpB"
                     /locus_tag="ACHLAG_004589"
     CDS             2535..4583
                     /gene="kdpB"
                     /locus_tag="ACHLAG_004589"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFP0657702.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVITLKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4592..5164
                     /gene="kdpC"
                     /locus_tag="ACHLAG_004590"
     CDS             4592..5164
                     /gene="kdpC"
                     /locus_tag="ACHLAG_004590"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFP0657703.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWCFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5157..7841
                     /gene="kdpD"
                     /locus_tag="ACHLAG_004591"
     CDS             5157..7841
                     /gene="kdpD"
                     /locus_tag="ACHLAG_004591"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFP0657704.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7838..8515
                     /gene="kdpE"
                     /locus_tag="ACHLAG_004592"
     CDS             7838..8515
                     /gene="kdpE"
                     /locus_tag="ACHLAG_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFP0657705.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
BASE COUNT         1791 a         2202 c         2475 g         2068 t
ORIGIN      
        1 gttttatcgg gttatttgtc tgcttccagg caaaacccta cagatttata agaaagcctt
       61 aagtgagata tctgtcagat aagaacaatt aggaaaatat ttatatatat ttcatgaata
      121 tatgtttcga ttggtataac gatgtctaaa tattcctctg aacatcttcc tgatacaaaa
      181 aaccgccagc gtttccgctg gcggttttca atgtattcaa aactcagcaa tcaataaaaa
      241 tcaccagttg ccttttcggc ctgatccatc cacaccggtt tatcgctggt tttcgaccag
      301 acgcgatgca ggtagctgta aaagcgggcg cggtctttcc agaaaagcat gaaaggcagc
      361 gccagaacgc ccgctgcaac cgcataagta cggcgtaaaa agataagcca tgcaggatat
      421 tctctgtaga gttccatact tttctccccc ttttgtatct acccggtgaa tggcaccgga
      481 aaaatgaatt tgtttatctg atgaaaatag taccgccttt tgtgtaattt tactactcat
      541 ccgaccactt atttttgctt attgatggtt tatttacatt catcctgtaa ttaagttaca
      601 caaaagttaa attaatacta aacattagtt aaatcatggc ttttgccatt tttatacttt
      661 ttttacaccc cgcccgcaga tttttgcgaa atctttgcag ccagaattct acccttccgg
      721 tatcactttt aggccactgg aggtgcactg tgagtgcagg cgtgataacc ggcgtattgc
      781 tggtgttttt attactgggt tatctggttt atgccctgat caatgcggag gcgttctgat
      841 ggctgcgcaa gggttcttac tgatcgccac gtttttactg gtgttaatgg tgctggcgcg
      901 tcctttaggc agcgggctgg cgcggctgat taatgacatt cctcttcccg gtacaacggg
      961 cgttgagcgc gtactttttc gcgcacttgg cgtctctgac cgtgagatga actggaagca
     1021 atatctctgt gccattctcg gcctgaacat gctggggctg gcggtgctgt tttttatgtt
     1081 gctcggtcag cactatctgc cgcttaatcc acagcagttg ccggggctgt cgtgggatct
     1141 ggcgctgaat accgccgtca gctttgtcac gaataccaac tggcaatctt acagcggtga
     1201 aaccacgttg agctatttca gccagatggc tggcttaacg gtgcaaaact ttctttctgc
     1261 cgccagcggg attgcggtga tttttgccct catccgtgcg tttacccgcc agagcatgag
     1321 cacactcggg aatgcctggg tcgatctgct acgcatcacg ttatgggtgc tcgtccctgt
     1381 ggcgttgttg atcgcactgt tttttattca acaaggtgcg ctgcaaaact ttcagcctta
     1441 tcaggctgtg aataccgttg aaggagcgca acagctgtta cccatggggc ctgtagcttc
     1501 tcaggaagcg atcaagatgc tcggtactaa cggcggtggc ttctttaatg ccaactcgtc
     1561 gcatccgttt gaaaacccaa ccgcactgac caacttcgtg cagatgctgg cgatcttctt
     1621 gatcccaacg gcgctgtgct ttgcctttgg tgaagtgacg ggcgatcgcc gccaggggcg
     1681 catgttgctg tgggcgatgt cagtgatttt tgtcatctgc gtaggcgtgg tgatgtgggc
     1741 agaagttcag ggtaatcctc atctgctggc gctcggcgca gacagcagca tcaatatgga
     1801 aggtaaagag agccgtttcg gcgtgctggt cagtagcctg tttgcggtcg tgactacggc
     1861 ggcttcctgt ggcgcggtga ttgcgatgca tgattcgttt accgctctcg gtggcatggt
     1921 gccgatgtgg ctgatgcaaa ttggtgaagt ggtgttcggc ggtgtcggtt ctggtcttta
     1981 cggcatgatg ctgtttgtcc tgctggcggt gtttattgcc gggctgatga ttggtcgtac
     2041 accggaatat ctgggtaaaa aaatcgacgt acgcgagatg aaactgactg cactggcgat
     2101 tctggttacc ccgacgctgg tgctgatggg cgcggcgctg gcaatgatga ccgacgccgg
     2161 acgtagcgcc atgctcaacc ctggcccgca tggttttagc gaagtgctgt acgccgtgtc
     2221 atccgccgct aacaacaacg gcagcgcctt tgccggatta agcgccaact ctccgttctg
     2281 gaactgttta ctggcgttct gcatgtttgt cggtcgcttc ggggtgatta tcccggtgat
     2341 ggcaattgcc ggttcgctgg tgagtaaaaa gagccaaccc gccagctccg gcacgctgcc
     2401 aacgcacggc ccgctgtttg ttggcctgtt aatcggcacc gtgttgctgg ttggcgcact
     2461 gacctttatc cctgccctgg cgcttggtcc agtggcggaa tatctctcct gatgatattg
     2521 agtgagcact gaatatgagt cgtaaacaac tggcgctatt cgaaccaaca cttgtcgttc
     2581 aggcgctgaa agaagcggtg aaaaaattaa acccgcaggc gcaatggcgc aatccggtga
     2641 tgtttatcgt ctggatcggc agtctgctga ccacctgtat tagcatcgcg atggcaagcg
     2701 gtgcgatgcc tggcaatgcg ctgtttagcg cggcaattag cggctggctg tgggtcaccg
     2761 tactgttcgc taatttcgcc gaggcgctgg cagaaggccg cagtaaagcg caggccaaca
     2821 gtcttaaagg ggtgaaaaaa actgcctttg cccgcaagct gcgtgagccg aaatatggcg
     2881 ctgcggcgga caaagttcct gccgatcaac tgcgtaaagg tgacatcgtg ctggtagaag
     2941 ctggcgatat tatcccctgc gatggtgaag tcattgaagg cggcgcatcg gtcgatgaaa
     3001 gcgccatcac cggggaatcg gcaccggtga tccgtgaatc cggcggcgat tttgcctccg
     3061 tcaccggcgg cacgcgtatt ctttctgact ggctggtgat tgagtgtagc gttaaccccg
     3121 gtgagacatt tctggatcgg atgatcgcga tggtggaagg cgcacagcga cgcaaaacac
     3181 cgaacgagat tgccctgact atcctgctga ttgctctgac catcgtcttt ttactggcaa
     3241 ccgccacgct gtggccgttt tccgcgtggg gcggtaatgc cgtgagtgta acggtactgg
     3301 tggcgctgct ggtctgtctg atcccaacca ctattggcgg cctgttgtca gcgatcggcg
     3361 tcgccgggat gagtcggatg ctgggcgcga atgtgattgc caccagcgga cgtgcagttg
     3421 aagcggcagg tgacgttgac gttctgctac tggataaaac cggcaccatc acactcggta
     3481 accgtcaggc gtcggagttt atccctgcac agggcgtgga tgaaaaaacg ctggctgacg
     3541 ccgcacaact ggcttcgctg gctgatgaaa cgccggaagg ccgcagtatt gtgatcctcg
     3601 ccaagcaacg ttttaacctg cgcgagcgcg atgtgcagtc gctccatgcc acctttgtac
     3661 cgtttactgc gcaaagccgg atgagcggga tcaacatcga caaccgcatg atccgtaaag
     3721 gttctgtcga tgccattcgc cgccatgttg aggctaacgg tggtcacttc cctaccgatg
     3781 ttgatcaaaa agtcgatcag gttgcgcgtc agggggccac gccgctggtg gtggtggaag
     3841 gttcccgtgt gctgggcgtt attacgctga aagatatcgt caaaggcggt attaaagagc
     3901 gcttcgccca gctgcgcaaa atgggcatta aaacggtgat gattaccggc gataaccgtc
     3961 tgactgccgc cgccattgct gcggaagcgg gcgtcgatga ttttctcgcc gaagcgacac
     4021 cggaggccaa gctggcattg attcgtcagt atcaggcgga aggccgtttg gtggcgatga
     4081 ccggcgacgg caccaacgat gctccagcgc tggcgcaggc agatgtcgcg gtggcgatga
     4141 actccggtac tcaggcggcg aaagaagcgg gcaatatggt cgatctcgac tctaacccga
     4201 ccaagctgat cgaggtggta catattggca aacagatgct gatgacccgt ggctcgctga
     4261 ccaccttcag cattgccaac gatgtggcga aatacttcgc cattattccg gcggcgttcg
     4321 cggcaacgta tccgcagtta aatgcgctga acatcatgcg ccttcattcg cccgactccg
     4381 ccattctcag tgcggtcatt ttcaacgcct tgattatcgt ctttttgatt cctctggcgt
     4441 taaaaggcgt gagttataaa ccgcttaccg cttctgccat gttgcgccgt aacttatgga
     4501 tttacggtct gggtgggctg ctggtgccgt ttattggtat caaagtcatt gatttactgc
     4561 tgaccgtttg cggtctggtg tgaggtttac catgcgtggc ttacgtccgg cattatcgac
     4621 atttatcttt ctgttattga ttactggcgg cgtttacccg ctgctgacca ccgtgctggg
     4681 gcaatggtgt tttccctggc aggccaacgg ttcgttgatt cgtgaaggtg atacggtgcg
     4741 gggttcggca ttaatcgggc agaattttac cggcaacggc tattttcagg gtcgcccgtc
     4801 ggcaacggca gaaatgccct ataatccaca ggcttctggc gggagcaatc tggcggtcag
     4861 taaccctgag ctggataaac aaatagccgc acgcgttgct gcattacggg ccgctaaccc
     4921 ggatgccagt acgaacgttc cggttgaact ggtgacggca tcggcaagcg ggctggacaa
     4981 taatatcacc ccgcaagcgg cggtctggca aatcccacgc gtggcgaaag cgcgtaatct
     5041 cagcgttgaa cagctcacgc aactgatcgc aaaatacagc caacaaccgc tggtgaaata
     5101 tatcggccag ccggtcgtca acattgttga actcaatctg gcgctggata aacttgatga
     5161 ataacgaacc cttacgtccc gaccccgatc gtctgctgga acaaactgcc gcgccgcatc
     5221 gggggaagct gaaagttttc ttcggtgcct gtgcaggcgt cgggaagacc tgggcgatgc
     5281 tggcagaagc ccagcgactg cgggcgcaag ggctggatat tgtggttggc gtggtagaaa
     5341 cccatgggcg gaaagatacc gccgccatgc ttgaagggct ggctgttctg ccgcccaaac
     5401 gccaggcgta ccgtgggcgg catatcagcg agtttgatct cgatgccgcc ctcgcccgcc
     5461 gtccggcgct gatcttaatg gacgaactgg cgcacagtaa tgcgccaggt tctcgtcatc
     5521 ctaaacgctg gcaggatatc gaagaactgc tggaagctgg cattgatgtt ttcacaaccg
     5581 tcaacgttca gcatctggaa agtctgaatg atgtggtcag cggcgtcacc ggaattcagg
     5641 tacgggaaac cgtgcccgat ccttttttcg atgccgccga cgacgtagtg ctggtggact
     5701 tgcccccgga cgatctgcgc cagcggctga aagaaggcaa agtctatatt gccgggcagg
     5761 cggagcgcgc cattgaacat tttttccgca aaggcaatct gatcgccctg cgtgaactgg
     5821 cactgcgccg tactgccgat cgcgtagatg agcaaatgcg cgcctggcgg gggcatcctg
     5881 gtgaagagaa agtgtggcac acgcgcgacg cgatcctttt atgcatcggg cataacaccg
     5941 gcagtgagaa actggtccgc gcggcggcgc ggttggcctc gcgcctgggt agcgtctggc
     6001 acgcggtgta tgttgaaacc cctgccctgc atcgcttacc ggaaaaaaaa cgtcgggcaa
     6061 ttctcagcgc cttacgtctg gcgcaggagc tgggcgcgga gacggctaca ctttctgatc
     6121 cagcggaaga aaaagcagta gtgcgttatg cccgtgaaca taatctcggc aagattattc
     6181 tcggtcgccc ggcctcgcgc cgctggtggc gtcgggaaac gtttgctgac cgactggcgc
     6241 gcatcgcccc cgatctcgat caggtgctgg tcgcgcttga tgaaccaccc gcccgcacga
     6301 ttaacaacgc gccggatagc cgctctttta aagacaagtg gcgggtacag attcagggat
     6361 gcgtggttgc cgccgcgtta tgcgccgtta tcactttaat tgccatgcag tggctgatgg
     6421 cgtttgatgc cgccaacctg gtgatgctgt atctgcttgg cgtggtggtg gtggcgctat
     6481 tttatggacg ctggccttcg gtggttgcta ccgtcattaa tgtggtgagt ttcgatctct
     6541 tttttatcgc cccacgcggc acgctcgctg tctctgatgt gcaatatctg ctgaccttcg
     6601 cggtgatgtt aaccgtcggg ctggtgatcg ggaaccttac tgcgggtgtg cgttatcagg
     6661 cgcgggtagc ccgttaccgc gagcaacgca cacggcactt atatgaaatg tcgaaagctc
     6721 tggcggtggg ccgcagtccg caggacatcg ccgccaccag cgaacaattt attgcctcca
     6781 cgtttcatgc ccgcagtcag gtgttgttgc ccgatgacaa cggtaaattg cagccgttaa
     6841 cacatccgca aggaatgacg ccatgggacg atgccatcgc gcagtggagt tttgataaag
     6901 gcctgcctgc gggtgcgggc accgacacgt tacccggcgt gccgtaccag attttgccgc
     6961 taaaaagcgg cgagaaaacc tacggactgg tggtggtgga accggggaat ctgcgtcagt
     7021 tgatgatccc ggagcagcag cgcctgctag agacgtttac gctgttagtc gccaatgcgt
     7081 ttgagcggct gacgctgacc gccagtgaag aacaggcgcg gatggcaagc gaacgtgaac
     7141 agatccgcaa cgccctgctg gcggcgcttt cgcatgattt acgcacgccg cttacggtgc
     7201 tgtttggtca ggcagaaatc ttaacgctcg atctggcaag cgaaggatcg ccccacgccc
     7261 gccaggccag cgagattcgt cagcatgtgc tgaacactac ccgactggtg aataatctac
     7321 tggatatggc gcgaattcag tccggcggct ttaatttgaa gaaagagtgg ttaacgctgg
     7381 aagaagtagt cggcagcgcg ctgcaaatgc tggaaccggg tttatcgtcg cccatcaatc
     7441 tttctctgcc agaaccgctg accttaatcc acgttgacgg gccactcttt gaacgggtgc
     7501 tgattaatct gctggagaac gcggtgaaat atgcgggtgc gcaggccgaa attggtatcg
     7561 atgcccacgt tgagggcgaa aatctacaac tggatgtctg ggataacggc cccggtcttc
     7621 cgccaggcca ggagcagacg atatttgata agtttgctcg cgggaataaa gagtcggcag
     7681 taccgggggt agggcttgga ctggcaattt gtcgggcgat agtggatgta cacgggggca
     7741 ctattaccgc gttcaaccga ccggaaggtg gtgcctgttt tcgtgttaca cttccccagc
     7801 aaaccgctcc tgaacttgaa gactttcatg aggatatgtg acaaacgttc tgattgttga
     7861 agatgaacag gctattcgtc gctttctgcg cacggcgctg gagggcgacg ggatgcgcgt
     7921 ctatgaggcc gaaacgctgc aacgcggctt gctggaagcg gctacccgta agccagattt
     7981 gattattctc gatctcggcc tgcccgatgg tgatgggatt gagtttatcc gcgacctgcg
     8041 ccagtggagc gcggtgccgg tgattgtgct ttccgcacgc agcgaagaga gcgacaaaat
     8101 cgccgcgctg gatgccggag cggatgatta tctgagtaag ccgtttggca ttggcgaatt
     8161 gcaggcccgt ctgcgcgtcg ctttacgccg ccactccgcc accgccgcgc ccgatccgct
     8221 ggtgaaattt tccgatgtta ccgtcgattt agccgcccgc gtgattcacc ggggtgagga
     8281 agaggtgcat ctcacgccga ttgagttccg cctgctggcg gtgctgctga ataatgccgg
     8341 gaaagtgctc acccagcgcc agttactcaa tcaggtgtgg gggccaaacg cggtcgaaca
     8401 cagtcactat ttgcgtattt atatgggaca tctgcgacaa aaactggaac aggatcccgc
     8461 tcgcccccgc catttcatta ctgaaaccgg tattgggtat cggtttatgc catgaatgtt
     8521 tattcaataa atgcgc
//
LOCUS       DAZGVQ010000197         8527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000197 DAZGVQ010000000
VERSION     DAZGVQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.199"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..2674
                     /gene="cas3"
                     /locus_tag="ACHLAG_004593"
                     /pseudo
     CDS             1..2674
                     /gene="cas3"
                     /locus_tag="ACHLAG_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
     gene            2772..4334
                     /gene="casA"
                     /locus_tag="ACHLAG_004594"
                     /gene_synonym="cse1"
     CDS             2772..4334
                     /gene="casA"
                     /locus_tag="ACHLAG_004594"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFP0657706.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKNHGRWDDIWQDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFDELKVKATSIASLLPDAPGKQTKERNTDHFIKRDTTQHLCLHCVPLALFSIQLNA
                     PIGGRGYYPGLRGGGPLTTLIELLEYQGNQQTPLWRKLWLNVMPQDEADLPLPKTFDD
                     LVFPWLAPTRTSELDGAVVTDEQVNKLQAYWGMPRRIRIDFKTTSIGNCDICGRQSDA
                     LLGLMSLKNYGVQYVMWRHPLTPYRLPLKEGGDFYSVKPQPGGLIWRDWLGLIEVGNS
                     KNNTELPAQVVKLLNASNLKQTRVGLWGFGFDFEDMKVRCWYEHHFPLLLNKKEDLIP
                     KLRLAAQAASHILSLLHRALKEAWFSEKKTTKLDFGFVDIDFWNKTQHRFLRLVRKIE
                     EGQDPYELLSKWQKEMWLFARQDFDDRVFTNPYEPVDLERVMTARKKYFTTSAEKQNA
                     NAAREKKQETAE"
     gene            4331..4864
                     /gene="casB"
                     /locus_tag="ACHLAG_004595"
                     /gene_synonym="cse2"
     CDS             4331..4864
                     /gene="casB"
                     /locus_tag="ACHLAG_004595"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFP0657707.1"
                     /translation="MSIVKDEHKVALRKWHEELQDKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMKKGRFTRLL
                     AVKTPDDLLRQLRRAVKLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQN"
     gene            4879..5952
                     /gene="cas7e"
                     /locus_tag="ACHLAG_004596"
     CDS             4879..5952
                     /gene="cas7e"
                     /locus_tag="ACHLAG_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFP0657708.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDSGSPKTVILGGATRLRVSSQSL
                     KRAWRTSELFEQALAGHIGVRSGRIAREAATILIEKGIEDKKAIEWAVEIADYLGKAK
                     KDKKQKNDKKPKDPLTSAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEKDLALLRK
                     DRMAVDIAMFGRMLAKKPEFNVEAACQVAHAFGVSETIVENDFFTAVDDLRQASEDAG
                     AGHVDETGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNS
                     FASRAYASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKT
                     ECASFDVMNKQGSMKDVLDFICA"
     gene            5963..6709
                     /gene="cas5e"
                     /locus_tag="ACHLAG_004597"
     CDS             5963..6709
                     /gene="cas5e"
                     /locus_tag="ACHLAG_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFP0657709.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            6691..7341
                     /gene="cas6e"
                     /locus_tag="ACHLAG_004598"
     CDS             6691..7341
                     /gene="cas6e"
                     /locus_tag="ACHLAG_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFP0657710.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFVQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            7338..8258
                     /gene="cas1e"
                     /locus_tag="ACHLAG_004599"
     CDS             7338..8258
                     /gene="cas1e"
                     /locus_tag="ACHLAG_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFP0657711.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            8258..>8527
                     /gene="cas2e"
                     /locus_tag="ACHLAG_004600"
     CDS             8258..>8527
                     /gene="cas2e"
                     /locus_tag="ACHLAG_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFP0657712.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVS"
BASE COUNT         2147 a         1974 c         2333 g         2073 t
ORIGIN      
        1 tgaaggataa aaatattgtg acttttttga tttctgggga aaaacccgac gcggcgagaa
       61 agacgggggc gacgactatc accttctttg ctggcattcg ctggatgtag ccgcgatggg
      121 ctatttaatg gttaaaagaa attgcttcgg gctggctgat tattttcgtc aattagggat
      181 ttctgacaag gaacaggcgg ctcaattttt cgcctggttg ctgtgctggc acgatattgg
      241 aaaatttgcc cgctcttttc aacaacttta cctgccccct gaactcaaga ttcaggaagg
      301 tgccagaaaa aattacgaaa aaatctctca ttcaacgctg ggctactggt tgtggaatca
      361 ttatttaagt gaatgtcagg agttacttcc ttcatcttcg ctctctccgc gcaaacttag
      421 gcgagtgata gaaatgtgga tgcccgtgac gacagggcat catggtcggc cccctgatcg
      481 tatggatgaa ttggataatt ttctgcctga agacaaagct gcagcacgag attttctcct
      541 tgaaatcaaa ccactgtttc cgctcataga gatccccgca ttctgggatg atgatgaggg
      601 cattgaactt ataaaacacc tttcctggta tatctctgca accgtcgtgc tcgcagactg
      661 gacgggttcg tcaacgcgat tttttccacg cgtcgcacac ccaatggata ttaaaggtta
      721 ctggcagaaa actttaattc aggctcaaaa cgccttaacc gtctttcctc taaaagcaaa
      781 agtcgcgcct ttcaacggaa ttaatacgct gtttcctttt attgagaatc cgacaccgtt
      841 acagcaaaag gtgctggatc tggatatcag ccagcaagga ccgcagttat ttattctgga
      901 agatgtgact ggcgcaggta aaacagaagc ggcgcttatc ctggcgcaca ggttgatagc
      961 tgcggggaaa gctcaggggc tgttttttgg tctgccaaca atggcaacgg ccaatgccat
     1021 gtacgatcgg ctggtcaaaa cctggcttgc tttctattcg ccagaatccc gccccagcct
     1081 ggtgctggca catagtgccc gcacattaat ggaccgcttt aatgaatcac tctggtctgg
     1141 tgatttagtc gggtcagaag aaccggatga acaaacgttc agtcagggat gtgcggcctg
     1201 gtttgccgac agtaacaaga aggcgttgct ggctgaaatt ggcgtcggca cgctggatca
     1261 ggcgatgatg gcggtgatgc cgttcaaaca taataatctg cggcttctgg ggctgagtaa
     1321 caaaatcctg ctggctgatg agatccacgc ctgtgacgct tacatgtcgt gcattcttga
     1381 agggttgatc gagcggcagg cgcgtggcgg aaacagcgtt attttgcttt ctgccacgtt
     1441 atctcaacag cagcgcgaca aactcgtcgc tgcctttgcg cggggtacag agggcctgca
     1501 agaagctccg ttcctggaaa aggatgatta cccctggctg acgcatgtca cgaaatccga
     1561 tgtgaactca caccgggtag cgacgcgcaa agacgttgag cgtagcgtca gcgtgggttg
     1621 gcttcatagt gaacaagagt gtattgcgcg tatcgaatcg gcggtaagtc aggggaaatg
     1681 catcgcctgg atccggaatt ctgtcgatga cgctattaag gttcatcgtc agctgcttgc
     1741 ccgcggcgtc attcctgctt ccagcctttc actttttcat agccgctttg cttttagcga
     1801 tcgccagcga attgaaacgg agacactggc acgctttggt aaatattgta gtttacagag
     1861 agcgtcgcaa gtcattgttt gtacacaagt aattgagcaa agcgtggata ttgacctgga
     1921 cgaaatgatc tccgacctgg cccctgttga tttgctgatt cagcgagcag gacgattaca
     1981 gcggcatatc cgcgatatta atggtcagtt aaagcgtgac ggaaaggacg agcgttcccc
     2041 ccctgaattg ctgattctgg ctcccgtctg ggacgactct cctggcgacg aatggtttgg
     2101 cagtgccatg cgtaacagtg catttgtcta tcccgatcat gggcgaatct ggctgacgca
     2161 gcgtgtgctg cgtgagcaag gtgctattca gatgccacac gcagcccggc ttcttattga
     2221 atccgtctac ggtgaggacg tggtaatgcc ggaaggattt gcccgcagcg agcaggagca
     2281 agtgggcaaa tattactgtg atcgcgcaat ggctaaaaag tttgtgctga acttcaggcc
     2341 cggctatgcc gccaatatca acgattacct cccggaaaaa ctgtcgacac gtctggctga
     2401 ggagtctgtt tccctgtggc tggctacctg tattgacggt gtggtgaagc cttatgccac
     2461 cggtgctcac gcatgggaaa tgagcgttgt cagagtgcgc cgaagctggt ggaaaaaaca
     2521 tcgggatgag ttttctttac tggagggaga ggcgttcagg ctgtggtgca ttgaacagcg
     2581 acaagatccg gagatggcaa acgtgatttt agtcaacgat gacgaaagtt gcgggtattc
     2641 ggctacggaa ggattgattg gcaagattgg ttgagatgtc tgcgacagga ctctctgaat
     2701 agttgataca ccgaaaagga tggaaagatg gacaaccgac ctgggatggt cttttttacg
     2761 agaggatgcg tatgaactcg ttttcacttc tgacaacccc gtggttgcct gttcgtttta
     2821 aagacggaac gacaggcaag ctggcaccag tcgatctggc tgatgaaaat gttgtcgata
     2881 tcgctgcgcc gcgggcagat ctccagggtg cggcctggca gtttttgctg gggctactac
     2941 aaaccagctt cgcgcccaaa aatcacggtc gttgggatga tatctggcaa gacggactgg
     3001 aggctgaaaa gctacgggaa gcattgctgt ccttagaaca cgctttccag tttgggccag
     3061 attccccttc atttatgcag gattttgatg aattaaaagt taaggcaact tctattgctt
     3121 cgcttcttcc tgacgcacca ggaaaacaaa caaaagaacg aaatacagac cattttatta
     3181 agcgagacac aactcagcat ctgtgcctgc attgtgtgcc tttagcgctt ttttcaatcc
     3241 agttgaatgc acctataggt ggacgcggtt actatcctgg gctacgtggc ggagggcctc
     3301 tgacgacact tatagaattg ctggagtatc agggcaatca acaaacccct ttgtggcgaa
     3361 agctgtggct aaacgtaatg ccgcaagatg aagccgattt acctttacca aaaacctttg
     3421 atgacctggt ctttccttgg cttgcaccga cgcgtaccag cgaactggac ggtgctgtgg
     3481 taacggatga acaagtcaat aaacttcagg cctactgggg aatgccacga cgtatacgta
     3541 ttgattttaa gacaacctca ataggtaact gcgacatttg cggtaggcag agcgatgctc
     3601 ttttgggctt gatgtcactc aaaaactatg gtgttcaata cgtcatgtgg cgccatcctc
     3661 taacacctta ccgtctgccg cttaaagagg gtggagattt ttactcagtt aaaccacaac
     3721 ctggtggttt aatctggcgc gactggttag gcctcataga ggtaggtaac tcaaaaaaca
     3781 atactgaact ccccgcacag gtcgtgaaac ttttgaatgc cagcaatcta aagcagacaa
     3841 gggtgggctt atggggattt ggttttgatt ttgaagatat gaaggttcgt tgttggtatg
     3901 aacaccattt cccgctgttg ttaaacaaaa aagaagacct aatacctaag ttgcgtctgg
     3961 cagcgcaagc ggcatcacac attctaagct tgttacaccg agcgttgaaa gaagcatggt
     4021 tctctgagaa gaaaaccaca aagcttgatt tcggttttgt ggatattgac ttctggaaca
     4081 aaactcagca tcgattcctg agattagtgc gcaaaattga agaaggtcag gatccgtacg
     4141 aattactcag taaatggcaa aaggaaatgt ggttattcgc gcgtcaggat tttgacgatc
     4201 gtgtctttac caatccatat gagcccgttg atttggaacg cgtcatgacc gcacgcaaga
     4261 aatattttac aacatcagcg gagaagcaaa atgctaacgc cgccagggag aaaaagcagg
     4321 agactgctga atgagtattg taaaagatga gcataaagtt gcgctgcgta aatggcatga
     4381 agagttgcag gataaacgcg gcgagcgtgc cagtctgcgg cgtagtacga cggttaatga
     4441 tgtttgtctg acggatggat ttcgcctgtt tcttaagaac agacaaataa agtggcagga
     4501 tgaacccgag tggcgtatca ccgcactagc gctgatagca gcagttagcg ccaatgttaa
     4561 agctattgac gaacggcagc cttttgccgc gcaactggcg gcggtaatga aaaaggggcg
     4621 gtttactcgt ttgttggcag taaaaacgcc ggatgattta ttgcggcagt tacgccgtgc
     4681 ggtcaaatta ttaaacggtt ccgtcaatct tgactctctg gcagaggggg tcttccgctg
     4741 gtgccaggag agcgatgacc tgctcaatca tcaccgccgc cagcagcgac caacagaatt
     4801 tatccgtatc cgctgggcgc tggaatatta ccaggctggc gacgccgata acgaacaaaa
     4861 ttaataaagg actatctcat gacaacattt attcagcttc atttgttaac cgcttaccct
     4921 gctgccaatc ttaaccgtga tgattccggc tctccgaaaa ctgtgattct gggtggagca
     4981 acgcgtctgc gcgtttcctc gcaaagtctg aagcgtgctt ggcgcacttc tgaacttttt
     5041 gaacaggcgc tggcgggcca tattggtgtt cgtagcgggc gtattgcgcg tgaggcggca
     5101 actatcctga ttgagaaagg aatcgaagat aaaaaagcca tcgaatgggc agtagagata
     5161 gccgattatc ttgggaaagc caaaaaagac aaaaaacaaa aaaacgacaa aaaaccaaaa
     5221 gatccgctca ctagcgccga aactgaacag ctcgtccata tcagcccggc agaatttgac
     5281 gccgtaaaag cgctggccca tcaactggca gaagaaaagc gcgcgccaaa agagaaagat
     5341 ctcgctttgt tacgtaaaga ccgtatggcg gtagatatcg ctatgtttgg tcgtatgctg
     5401 gcgaaaaaac ccgagtttaa tgtagaagcc gcctgccagg tcgcgcatgc gtttggtgtc
     5461 agtgaaacga ttgtcgaaaa tgattttttc accgccgtag atgatttacg ccaggcttct
     5521 gaagatgccg gtgccggtca tgtagatgaa accggattcg gttctgcgct gttctacacc
     5581 tatatctgca ttgataaaga tctgctggta gaaaacctcg gcggtgacga agcgttagct
     5641 aatcagacca tacgcgcctt tacggaagcc gcgcttaaag tctccccaac cggtaaacaa
     5701 aacagctttg ccagccgggc ttatgcctcc tgggcgctgg cagaaaaagg caccgatcaa
     5761 ccgcgttctc tggcggcggc tttctatgaa cccattaatg gcacacgtca gttagatgtg
     5821 gcggtgcagc gtattacaac gcttcgcgaa aacatgaata cggtctacga acagaagacc
     5881 gaatgcgcaa gttttgacgt gatgaacaaa cagggaagca tgaaggacgt gctggacttt
     5941 atctgcgcgt aaggggcggc ttatgagcca atatctgatt tttcagcttc atgggccgat
     6001 ggcgtcctgg ggggtcgatg ctccaggtga agttcgtcat acccatgaac ttccttcgcg
     6061 ctcagcattg ctggggctac tggctgccgg ggtagggatt cggcgtgatg ataccgaacg
     6121 cttaaacgcg tttaatcgcc actattcact ggtggtttgc gccagccgta acccgcgctg
     6181 ggcacgggat tatcacacgg tccagatgcc aaaagaggtg cgtaaagcac gttatttcag
     6241 ccgtcgcgaa gagttgagcg accctgagct tttgagcgcg attatctccc ggcgcgacta
     6301 ctacaccgat gcctggtgga tggtggcggt ggcaacaaca gccgatgcgc cttacagcct
     6361 cgaacagttg caggacggtt tacgccatcc agtttttccg ctttatctgg ggcgaaaaag
     6421 tcatccgctg gcattacctc ttgcgccgtt actgctcgaa ggcaatgcgt gtgatgcctt
     6481 atgtaatgca taccaacagt atcaggatca tttccataaa ctgaaagtct cactcccgaa
     6541 acttcaggat gaatgctggt gggaagggaa acacgacggc ctggtcgcga gcaaaatatt
     6601 acgccgccgg gatgttcctt taaatcgtca gcagtggctg tttggggaac gcaccgtcaa
     6661 tcaggggccg tggctcagca aggaggaacc atgtacctct caagaataac cttacatacc
     6721 ggccagcttt cgcctgcgca gttgctgcat ctggtggatc gcggtgaata tgtgatgcat
     6781 cagtggctgt gggatctctt ccctggcggc aaagaaaggc aatttcttta tcgtcgggaa
     6841 gaactccagg gcgcgtttcg cttttttgta ctgtcgcagg aacgcccggc ggaaagtgat
     6901 acgttcacca tagaatgccg gtcatttgcg cccgagctgt gtaccggaca acaactttgt
     6961 ttcaacctgc gggcgaatcc aacaatctgt aaagcgggca agcgccacga tctgctgatg
     7021 gaggcgaaac ggcaggtgag ggggcaggcg gaaggaagtg atgtctggtt gcatcaacaa
     7081 caggcggcgc tggactggct ggcagcgcag ggggagagaa gcggttttac actgctggat
     7141 acctcggttg atgcctaccg acaacagcaa ctgcggcggg aaaactcccg gcaactgatc
     7201 cagttcagta gcgtggatta tacggggatg cttacggtca ccgatcctgg gttatttgtg
     7261 caacgcctga gccaggggta tggtaaaagc cgggcgttcg gctgtggtct gatgctgatc
     7321 aaacccggag cagaggcgtg acgtttgtac cactgagccc gatcccatta aaagatcgca
     7381 cctccatgat cttcctccag tacggtcaga tcgacgtact ggacggcgct ttcgtgctga
     7441 tcgacaaaac agggatccga acgcatattc cggtgggatc ggtcgcctgc attatgctcg
     7501 aaccaggaac gagagtttcc cacgcggcgg tgcatctggc cgccacggtg ggaacactgc
     7561 tggtctgggt cggtgaagcg ggcgttcgcg tttactcctc cggacaaccc ggtggggcgc
     7621 gggcagataa attactctac caggcaaagc tggctttaac ggaagatcta cggttgaagg
     7681 tggtgcgcaa aatgtatgaa ttacgttttc gtgaaccaca gccagctcgc cgttcagtgg
     7741 agcagttacg tggaattgag ggatctcgcg ttcgccagac gtatgcatta ctggcgaaac
     7801 aatatggtgt gaaatggaat ggtcgcaaat acgatcctaa agactgggaa aaaggcgatg
     7861 ttgtgaatcg ctgcatcagt gctgctacat catgtctgta cggtatatct gaagcggcag
     7921 tattagccgc gggatatgcg cccgctattg ggtttatcca tagtggcaaa ccgctttcat
     7981 ttgtttatga catagccgat atcattaaat ttgattcggt tgtgccaaag gcatttgaaa
     8041 tagcagcgag gcaacccgca gaacctgata aagaagtcag attagcctgt cgcgatattt
     8101 tccgtagcac taagttaacg ggcaaattaa taccgttaat tgaggaagtc cttgccgcag
     8161 gtgaaattga accgccacaa cctgcgccgg atatgttacc gccagccatc cccgaacctg
     8221 aaacgttggg cgatagcggt catcgaggac gcggctgatg agcatggtcg tggttgttac
     8281 agaaaatgtc ccgccgcgct tacgtggacg gctcgcaatc tggctactgg aagtgcgtgc
     8341 cggtgtgtat gttggtgata catcaaaacg tattcgggag atgatctggc agcaaattac
     8401 ccaactggct ggttgcggaa atgtggtgat ggcctgggcg accaataccg agtcgggttt
     8461 tgaatttcag acctggggag aaaacagacg tattccggtg gatttggatg ggttacgttt
     8521 ggtttct
//
LOCUS       DAZGVQ010000198         8521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000198 DAZGVQ010000000
VERSION     DAZGVQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.200"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..574)
                     /gene="yeeO"
                     /locus_tag="ACHLAG_004601"
     CDS             complement(<1..574)
                     /gene="yeeO"
                     /locus_tag="ACHLAG_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFP0657713.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTK"
     gene            735..810
                     /locus_tag="ACHLAG_004602"
     tRNA            735..810
                     /locus_tag="ACHLAG_004602"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:768..770,aa:Asn,seq:gtt)
     gene            complement(848..1798)
                     /gene="cbl"
                     /locus_tag="ACHLAG_004603"
     CDS             complement(848..1798)
                     /gene="cbl"
                     /locus_tag="ACHLAG_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFP0657714.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRRGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1900..2817)
                     /gene="nac"
                     /locus_tag="ACHLAG_004604"
     CDS             complement(1900..2817)
                     /gene="nac"
                     /locus_tag="ACHLAG_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFP0657715.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRKKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            3144..3219
                     /locus_tag="ACHLAG_004605"
     tRNA            3144..3219
                     /locus_tag="ACHLAG_004605"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3177..3179,aa:Asn,seq:gtt)
     gene            complement(3275..4210)
                     /gene="ldtA"
                     /locus_tag="ACHLAG_004606"
     CDS             complement(3275..4210)
                     /gene="ldtA"
                     /locus_tag="ACHLAG_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP0657716.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(4272..5351)
                     /gene="cobT"
                     /locus_tag="ACHLAG_004607"
     CDS             complement(4272..5351)
                     /gene="cobT"
                     /locus_tag="ACHLAG_004607"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFP0657717.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMLGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(5363..6106)
                     /gene="cobS"
                     /locus_tag="ACHLAG_004608"
     CDS             complement(5363..6106)
                     /gene="cobS"
                     /locus_tag="ACHLAG_004608"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFP0657718.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(6103..6648)
                     /gene="cobU"
                     /locus_tag="ACHLAG_004609"
     CDS             complement(6103..6648)
                     /gene="cobU"
                     /locus_tag="ACHLAG_004609"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFP0657719.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIHADINPHETVLLECVTTMVTNLLFDYGGDKDPGE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(6782..7141)
                     /locus_tag="ACHLAG_004610"
     CDS             complement(6782..7141)
                     /locus_tag="ACHLAG_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001516590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFP0657720.1"
                     /translation="MHCRGCGFIGEEWQQDFQPLDGGPVQSLSDAYSDYSVLLLIAAE
                     SEISVPEWFPHSEGTTVILIAEHNARLLTGPAGGAVALIHYVNEICHSRETAVNDAFC
                     RTLTRGSLAIPQCHLLS"
     gene            7120..7416
                     /locus_tag="ACHLAG_004611"
                     /pseudo
     CDS             7120..7416
                     /locus_tag="ACHLAG_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
     gene            complement(7517..7846)
                     /gene="tmaR"
                     /locus_tag="ACHLAG_004612"
     CDS             complement(7517..7846)
                     /gene="tmaR"
                     /locus_tag="ACHLAG_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFP0657721.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(8018..>8521)
                     /locus_tag="ACHLAG_004613"
     CDS             complement(8018..>8521)
                     /locus_tag="ACHLAG_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657722.1"
                     /translation="QSAFSPNLLERPRLESHLQKLLTDAVKMRGLIAPASKESRIPKS
                     IYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQQILLSLVHALY
                     EGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNMETAHQLELLSNL
                     ICRALRK"
BASE COUNT         2264 a         2260 c         1896 g         2101 t
ORIGIN      
        1 ttttcgtatt ccctgcacca cgaagtgccc cgctaccaat caaagtgatg gcagctgctg
       61 gataactgag taccgtcagc tccagataag tcaacgccag tgctttaact tctgtcgtgg
      121 catcacccgc gacgaaatca ataatttgtt cgccaaaatg atgaataagc gttgccaaca
      181 gtacggcaaa caacgtcatg atcaccaatg actgccgcgt cgccaccctc gctcgtcgtc
      241 gatcccgctt accgagacta aatgccacaa cgacagtagt accaagatcg atagctgcaa
      301 aaaaagccat aatgaccata ttgaagctgt ccgccaatcc cacgccggcc atcgcatctt
      361 ttcccagcca gctgaccaga aaagtgctca ggacacccat caacaggaca caggcattct
      421 ccatgaagat aggaacagca agcggggtta tctcgcgcca gaacaacact ttgtagctct
      481 tgcgtttagc gtgccagcga gtgccgtgaa caacctggcg taaagcagag gagatattca
      541 aagccgacct taattgcaga aagtgaaacc acatttcaaa taatgaggga gaatcagcaa
      601 agctgcaaag attttcgcca acaaattgtc tgcaaatgca acaaactgtt gatagaaacg
      661 gcaaacagtt ggggaattta aaaatcgggt ttgacaaaag atttttcgcc gttaagatgt
      721 gcctcaacag cgattcctct gtagttcagt cggtagaacg gcggactgtt aatccgtatg
      781 tcactggttc gagtccagtc agaggagcca aatttctgaa aagcccgctt ttatagcggg
      841 ctttttgcta tatctgataa tcaatttcct cttcactgtt ttccatcacc tgccgcttga
      901 tatcctcaac tgacagtcct gcattacaaa gttccagaaa gcgccagaca tagttacgct
      961 gaagttgtcc tcgcttcagt cccaaccaga cagtattagc atcgaaaaga tgccgcgtat
     1021 ccagacggat taaattctct tcctcttgtt cgccgctgga ttgttcggca actaatccga
     1081 tcccaagccc aagagcaaca taggttttaa tgacatcaga atcctgcgca cttaatacaa
     1141 tatctgccag caaacctttg cgggcaaatg cgtcatcaat acgtgagcgc cccgtaatcc
     1201 cccgtcggta agtgattaac ggccacttcg ctattgattc cagcgtcaat ggtgtaattt
     1261 gcgttaaggg atgatcgagt ggaacaagca aactatggtg ccaacgaaac cacgggaagg
     1321 cgacgagctg cgggtcatta ctcaaacgct cgctggcgat accaatatca gcttcgccat
     1381 tttgcaacaa cgtcgcaatt tcctgtggcg tcccctggat tagctcgagc cgaacctccg
     1441 ggaaaagttc gcgaaaagct ttaatgacct ctggcaagct ataacgtgcc tgagtatgcg
     1501 tcgttgcaat agtgagaacg ccagacgtat cgttggtaaa caggtctgca agccgacgaa
     1561 cattactggc ttcattcaga atacgttctg caatgaccag taatgctttg cccggttcag
     1621 tcatgcccag cagtcgctta cctcgtcgaa caaatatttc gatgccaagt tcatcctcca
     1681 gttcccgaat atgacggctg acgcctgact gtgaggtaaa aagcatattc gcaacctctg
     1741 tcaggttgta atcctgacgt gcagcctcgc ggattatctt tagttgttgg aaattcacgg
     1801 taaactccgg gcagttcaga tttcccgtta ttgttaaagt ctaatgcccg gcataacaaa
     1861 taataaaaac ccgcatctta ttccattccg atataacact tagctcacca attgccactg
     1921 ccttttttcc atcactggag aactaatcac tgacattaac aactctttca ctgcctgtgc
     1981 ctgtggcgat aagttcgctc tggcgggtaa atttaatgac aaagagagac tcatggaagg
     2041 agtggtaatg cgtgacatcc acccatttac tgcgccacat aacgaacgcg cggccgattc
     2101 gggtaatact gcaacgccca tgccgctggc aatcgctgcg gtaagcgtgg caatagactc
     2161 aatttcacca ataacttttg ccgtgagtcg ccgtagggaa aaagcctcat caacacgaag
     2221 tctaatagca ctgtaatcac tggggagaaa gaggttcatt tgcgcaatag cattcacatc
     2281 aacgctttgc cccggacaat cttgagttcc taccaggaaa agatcttctt tcagcaaagc
     2341 ctgactggat accccagcca caggggaatg ctcataaatc accgccatat cgagttggtg
     2401 atttatcagt ttttcgttaa gcactgcacc gctattttca tgaagataga taacgatctc
     2461 cgggaattca gcgcgaaccg cctgtaataa gggcatggtg atggatgacg cagcggttcc
     2521 tggcgcaaag ccaatcgaga cttgccccga taatgcctga ccaacgttat gcaccgccag
     2581 ttgggcctgc tcacactgac gtaaaatggc ccgcgcatgg gtatagagaa tttttccggc
     2641 gtctgttggc gtaacgcccc gttttttgcg aatcaaaagt tgttgattta actcaccttc
     2701 cagtgtggca acctgctggc tgagcgctgg ttgtgcaata tgcaatactt cagcagcctg
     2761 ggtcaggcta ccaatatcta caatttttac gaagtatttc aggcgtctga agttcatgtt
     2821 gcctccggtt tttaagaatc ggcccaagtg ccgccattac ttacaaccag actgcaagat
     2881 gcttgccagt tttattttgg cgttgatgta caagctaacc aactgtcaaa taagagatta
     2941 tgatagattc gtcatttgct cctttaatca gctgtcgcgt tcccctgccc tataaaagga
     3001 gggtatgcac cacgatgatt cattacccaa taagattgaa agctcaccac tttgttgaaa
     3061 ttgacagcaa acaaacaaaa aaatgcattt caccctttga catcaccatg cactgccatt
     3121 aatatgcgcc ccgttcacac gattcctctg tagttcagtc ggtagaacgg cggactgtta
     3181 atccgtatgt cactggttcg agtccagtca gaggagccaa attcaaaaaa gcctgctttc
     3241 tagcaggctt tttgctttct aattaccaac gctcttaaaa catctgtctt gaaccagaac
     3301 taatttgcac aggcattccc gatcgacgtt gcaacgcagc atttgcgcga tttacatcaa
     3361 cttcttgccc gctgataaac gcccgcaaag atggggttac cggcaatggc acttttcggt
     3421 cagactcata ttctgcacga ttgcgcgaca acggctcatg aacttccagc cagttcgagc
     3481 catctggttc agtggtgtat tttactggct ggtcgataat ttgcacacgc gtcccaacag
     3541 gaacattatc aaacagatat ttgatatcgt cattgcgcag acgaatacag ccctgactta
     3601 cccggagccc aataccaaaa ttggcattgg taccatggat ggcatacaac ctgccaatat
     3661 aaatcgcgta cagccccatg ggattatcgg ggcccgcagg aacaaatgcg ggcaaactct
     3721 cccctcgttt cgcatattcg cgccgagtgt tcggcgttgg cgtccaggtt ggagcttctt
     3781 gtttacgttc aacggtagtc acccagttac gcggggtttc tcgcccagcc tggccgatac
     3841 caataggaaa gacttccaca gtattactgt ctggtgggta gtaataaaga cgcatctcag
     3901 cgacgttaac cacaatccct ttacgaacag tggcgggcaa aatcagttgc tgcggaatgg
     3961 tgagttgcga gccagacttc ggcaaaaaaa catcagcgcc cgggttcgct tccagcatgt
     4021 tacttaaccc ttgcccgtat tgtgcggcaa aagtctccag cggctgggta ttgtgatcag
     4081 gaacagttac agtaaacgac tgccccacta aacggctacc ctctggaggt aatggataag
     4141 ttaccgccag gctagtatgg ctggcaaaaa gtagagcaaa tgagcaaaaa atatttacac
     4201 gacgcatcat gtccctttcc taagtcgcga aagctatccg ttaattatag cttttatcag
     4261 acttttcgtt tttaactgtt caaatcagaa gtcgtattcc ccggtagaac aatattactg
     4321 gcagcaagtt cgcccatgtt gttatatatc gcacaggcag cttcgatgat gggcatcgcc
     4381 agagctgcgc cactcccctc acctaaacgc atctccatat tgagataagg ctccagcccc
     4441 aaatgcgaca gcgcaatacg cgcccctttt tctgccgaca ggtgagaagg aatgagatac
     4501 ggtttgattg caggagacat ctggcaggct gcgagcgcgg cagcataaga aagaaatcca
     4561 tccagcaaca cgggtaaacc gcaggaagca gcgcctaaca tcacgccagc cattccgacc
     4621 aaatcaaatc cacccacttt tgccaggaca tcaacgccat cctgcggatt tggttgattc
     4681 aacgtaatcg cccggcgcac aacatcaatt ttattagcca gtttatctgt cggcaggttt
     4741 gcgccaatcc caaccacttc ttcaggagcc cggccagtga ttgtgctgac tattgccgct
     4801 gccggtgtcg tgtttgccat ccccagttca cctacaccaa acagcgtaac accgtttttt
     4861 gccagctccc gcgtataaca tatgacgtcc aaaagcaact tttctgcctg atggcgactc
     4921 attgccggag ctgaagcaat attgccacta cctcgagcaa cacgcatgtt gataagcccg
     4981 gggataggct cagcagtatc aataccaaca tcaattacgt agacgttagc gcccgcttgt
     5041 gctgccagca cacacacgcc ggttgttccg cgggtcatat tttcagcctg aatggctgtc
     5101 acttcttttg gggaaatagc gaccccttcc tcccagacgc cgtgatcggc acacatgacc
     5161 agtaccgctt ttttgcccac atgcggaacg ccattcaacc ccagcattcc tgccagttgt
     5221 atggcaagcg cctccagcct tcccaggcta ccaacaggtt tgagtaaccc gtcgatatgc
     5281 cgttgtgcac gcgacatagc ggcaggatcg atagcaggga tcgtattcag taaatcggca
     5341 agtgtttgca tgtcacgtct ctttatagca gtgccagcag gaagaccagt tcaccaagct
     5401 ctatagcagc tcccagcgta tcgccggttt gcccgcccag cgtacgtttg agaagctgac
     5461 cgaggatgaa aatcgccacc atcgtcacca ccatagcagc cacaccatgc atacccggca
     5521 ataatacggc agcaaaaata gcggctaagc cgagtgtgac gcaggtttgt cgcccatcaa
     5581 ttttgccaat gaacacattg ccaagccctt cttcccgggc ataacgatga cgatacatca
     5641 ataatgcggc agtcccgcgg ctgaccgcac atgctgccgc cagcgatgca aggatcggtt
     5701 cgccacgcag agccaactcg cttaacacca gaatctttgc cagtacaaca aaaatcaatg
     5761 ccagaccgcc gtgggtgcct aaacggctat cacgcatgat ttccaacatg cgatcgcggc
     5821 tacgtgcaga aaatacgcca tcacaggtat cggcgaggcc gtccaggtgg aatcccccgg
     5881 tcatcagcgc cagcacaagt acgctaaaca gtgccgccag tggtacgcca caccatgcct
     5941 gcagcaccat gaagaccagc ccgctaatcg cgccaagcaa taatccaatt aaaggaaaag
     6001 taataatacc gcgagaataa tgctcgaaat ccagtccctg ggaccagcga cgcggtacag
     6061 gcagacgcgt aataaaagag agcatcgccc aaaataattt actcatttga tttttactcc
     6121 tatacccgaa accaccagcc atacttcatt tgccgcagcg gccagctgct gatttacccg
     6181 cccggcaata tcacgaaaat gtcgtgccag acgactctcc ggcacaatcc ccattcccac
     6241 ttcgttagtc actaatacaa cctttgcggg acaacgttgg caggcagcaa tcaacgactg
     6301 aatctcagca ttaatcgcct gttccatcgc ctgataatcc cattcaccag ggtctttatc
     6361 gccgccataa tcaaacaaca gattagtcac cattgtggta acgcattcca gcaatacagt
     6421 ttcatgcggg ttaatgtctg catgaattaa ttcatcaaga tgttgccaac gctccaccgt
     6481 gcgccagtgc gccgggcggc cttgccgatg atgttctatc cgtgcagcca tctcatcatc
     6541 aaggatttgt gaggtggcga tatacagaac ctgtgaagag tccccaataa gcgcctctgc
     6601 gtggcgactc ttcccgctcc gtgcgccccc cgtcaccaaa atcatcatac cgactcctga
     6661 ttcaattgta tgatggtatt cattgaacag aacaatggat cccccaccgt tgctgaccgc
     6721 aaaacattta cgcgattaat actgcgcgta atataattaa aaatccagca ttctcaatcc
     6781 attacgaaag aagatggcat tgcggaatag ccagacttcc tcgtgttaac gtccgacaaa
     6841 atgcgtcatt gacagcggtt tctctggaat gacagatttc attcacataa tggattaatg
     6901 ctacagcccc accagccgga ccagtcagca aacgggcatt gtgttctgca atcaaaatta
     6961 ccgtcgttcc ctcagaatga gggaaccatt ctggtacgga tatctcgctt tcagctgcta
     7021 tcaatagcag cactgaataa tcgctatacg catctgataa cgattgcaca ggcccaccat
     7081 caagaggctg gaaatcttgc tgccactcct caccgataaa accgcagcct ctacagtgca
     7141 ttgttggtca gtgcctgcga agcagacatt atcgcgctgg tgcttaatgc ggacgcaccg
     7201 tggtcacctt tttcgccagg ctttacctcg tcaatgaatc gcccggtcat tggagtagtc
     7261 accaaagcag atttagccag catggaacaa atatctttgg tgaaatgttg gttacgtgaa
     7321 gctggagcgc acaacgtgtt agtaaccagt gcggttaata acaatggagt tgcagaactt
     7381 tttgccttgc tgcatacaga agaaggctgt cgctaatttc gacaggttct cagatgagaa
     7441 tgatgacggt gatacacaga aaagaatcaa tgagcaatga gggttgccag gcaaccctca
     7501 ttgaataaaa caggaattac ttcgcttcgc cgtttttcat ttcgcccata gctttcagct
     7561 ttttggagat atcgcggcgt tctttggaga gctcggcatt tttgatgatg taatcgtcaa
     7621 cgcgatcttc atagtcacct ttcatgctgg cgatgatgcc ctggattgct tcaacgctca
     7681 tccccggctt gatgtaatcg ctcaggttgt ccagcagcag gacgcgcttc tggttgtcac
     7741 ggatcttttt ctcaacgtcc tgaatttcac gttgcagttt gttcttacga cggaacagac
     7801 gaacaaattc cagtacgtcc tggaatgaag gcttggtagt ttccattttt atacccctga
     7861 taatgtgaga gtcggattcg tttaagcaac cgctattcgt tagggccaac attactcata
     7921 gctgccgcag atgacaatgc ttttatccct tttcactatc atacccttta tccttgctga
     7981 atcgaagcag cagcaagatg attctgaagt tcaggaatta tttgcgcaag gcccggcaaa
     8041 tcagattcga tagcaactca agctgatgtg ccgtttccat gttcagccac acataaccat
     8101 agattggtgt ttccacaacc ttcaggtcat ggtggttatt gagcaactga cgcagctctt
     8161 ccacagcatc gttcaatttt tccgtattgg caaaaaccgg ctgcggatta ccttcgtaca
     8221 gcgcatgaac aaggctcagc agtatttgct gcatcatgtg ctgggtatca cgaagttttt
     8281 gcgcgttcaa taacacgaaa tggctggggc gcgtggccca gtacgcattg atttgcaact
     8341 ccagcataca aaccagattg cgattaatgg tctgaatacc ttcatatatc gattttggaa
     8401 tacgactttc tttgctggcg ggcgcaatca gtccacgcat tttcacggca tcggtcagga
     8461 gtttttgtag atggctttcc agacgtgggc gttcgagtaa gttcggtgag aatgcagatt
     8521 g
//
LOCUS       DAZGVQ010000199         8403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000199 DAZGVQ010000000
VERSION     DAZGVQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.201"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1387
                     /locus_tag="ACHLAG_004614"
     CDS             <1..1387
                     /locus_tag="ACHLAG_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HFP0657723.1"
                     /translation="SNKDFVIKFSTSVLQADKKEITIINKNENTTLTQTIAPIFEEYL
                     MEILPQRSDTLDKQELNLKSDRKEKEFPRIKLNGQCYFPGRPQNRIVCRHIAAQYIND
                     IYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALVSSRPEGRCVAACGDFGLVMKAY
                     FDKMESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPNVTNDKIRIMSE
                     SKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLMLKGSVDEITPPL
                     SPATMNLLMAIGQNHQLTQLMIQLQKMPELHRTEMLTAYNSGHMNVINTIFNALPTLF
                     NTFKFDKKNMKPLLLANNSNEYPGLFSAIQHKQQNVVETVYLALSNHARLFGFTAEDI
                     MDFWQHKAPQKYSAFELAFELGHRVIAELILNTLNKMAESFGFTDNPRYIAEKNYMEA
                     LLKKASPHTVR"
     gene            complement(1356..3092)
                     /gene="aceK"
                     /locus_tag="ACHLAG_004615"
     CDS             complement(1356..3092)
                     /gene="aceK"
                     /locus_tag="ACHLAG_004615"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFP0657724.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILL
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3373..4677)
                     /gene="aceA"
                     /locus_tag="ACHLAG_004616"
     CDS             complement(3373..4677)
                     /gene="aceA"
                     /locus_tag="ACHLAG_004616"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFP0657725.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4707..6308)
                     /gene="aceB"
                     /locus_tag="ACHLAG_004617"
     CDS             complement(4707..6308)
                     /gene="aceB"
                     /locus_tag="ACHLAG_004617"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFP0657726.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISEMASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSCLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6577..7506)
                     /gene="metA"
                     /locus_tag="ACHLAG_004618"
     CDS             complement(6577..7506)
                     /gene="metA"
                     /locus_tag="ACHLAG_004618"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFP0657727.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7663..8106
                     /locus_tag="ACHLAG_004619"
     CDS             7663..8106
                     /locus_tag="ACHLAG_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP0657728.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGHSEVDDLGKPYPLLNLAYVGE"
     gene            complement(8221..8336)
                     /gene="rrf"
                     /locus_tag="ACHLAG_004620"
     rRNA            complement(8221..8336)
                     /gene="rrf"
                     /locus_tag="ACHLAG_004620"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2142 a         2096 c         2076 g         2089 t
ORIGIN      
        1 tagcaacaag gattttgtca ttaaattttc cacgtccgtc ttacaagctg ataagaaaga
       61 aattacgata attaataaaa acgaaaacac gacacttact caaaccattg ccccaatatt
      121 tgaagaatac ctaatggaaa ttttacctca acgctcagac actcttgata aacaagaatt
      181 aaacctaaaa tcagatagaa aagaaaaaga attcccaaga attaaactta atggtcaatg
      241 ttattttccg gggcgacccc aaaaccgtat agtatgccga cacattgctg cacaatatat
      301 taatgatatt tatcagaatg ttgattacaa accccatcaa gatgattact cttcagctga
      361 aaaatttctc acgcacttca acaaaaaatg caaaaaccag actttggcgt tggtttccag
      421 ccgtcctgag gggcgttgcg ttgctgcctg cggtgatttc gggctagtta tgaaagcata
      481 ttttgacaag atggaatcaa atggcatcag tgttatggca gccatattac tggtggataa
      541 ccatgctttg acggtccggc taagaataaa gaacacaact gaaggatgta cccattacgt
      601 ggtttcggtt tatgatccta atgtaactaa cgataaaata agaattatga gcgaaagcaa
      661 agaggatatt aaacactatt ctctgatgga ttttatgaat gtagattata gcctcctgaa
      721 atggtcaaat gatcatgtta ttaaccaatc tgttgcaata attccagcac ttccgaaaga
      781 acagctattg atgttaaaag gatctgtgga tgaaataacc cctccattat caccagcaac
      841 gatgaatttg ctaatggcaa ttggtcagaa tcaccaactt acgcaactga tgattcagct
      901 ccagaaaatg ccagaactac atagaacaga aatgttgact gcctataata gtggacatat
      961 gaacgttatt aatactattt ttaacgcatt acccactctg tttaatacgt ttaaattcga
     1021 taaaaaaaat atgaagcccc tcctcctggc aaataattct aatgaatatc ccggtttgtt
     1081 ttcagcgata cagcataaac aacaaaatgt tgtagagacg gtttatcttg ctttatctaa
     1141 ccatgcacgc ctgtttggat ttaccgctga agatattatg gatttttggc aacacaaagc
     1201 cccacaaaaa tactctgcct ttgagttggc ttttgaattg ggtcaccggg ttattgctga
     1261 attaatcctt aatacattaa ataagatggc tgaaagcttt ggctttacgg ataaccctcg
     1321 atacattgcg gagaaaaatt atatggaagc tttactcaaa aaagcatctc cccataccgt
     1381 acgctaaatc tttgcctgcg ccgatacgca taaacatctt ccacatgccc ttcacgtatg
     1441 cggttttgta gtgcgcgcca gtaatcagcg cggaacaggt cggcgtgcat ctcttcaaac
     1501 agcggaccaa tacgcgggtc ggcgcatagc cagtggcgaa actcttccgg gaaaacatcg
     1561 cccggcgaga cgctgtacca cggttcgctg gcaagttcgt cttccggata gcgcggcggc
     1621 gggatgtcgc ggaaattcac ttccgtcatg tagcaaattt catcgtaatc ataaaaaacc
     1681 acacgcccgt gacgggtgac accgaagttt ttaaacagca tgtcgccagg aaaaatgtta
     1741 gcagcggcaa gctggcgaat agcgttaccg tattcttcaa tggcgtcgcg caactgctga
     1801 ccttccactt gttccagcca gatattgagc ggcaccatcc gccgctcgat atagagatgg
     1861 cgaattacaa tctgctcacc aagatcggtg attttttccg ctgcttcctg gagcagtaat
     1921 tccattaatg ccggggaaat atgccgcttc tccagcacaa agttttcaaa ctcctgggtg
     1981 tccgccattc ggcccacacg gtcgtgctct ttcaccagtt gatagcaggc acgaacgtga
     2041 gcggcagaca tctctttctg cggcgcgaac ttgtctttga tgactttgaa tacccgatca
     2101 aagcccggca gcgtaaatac caacatcacc attccacgaa tacccggcgc ttcaatgaac
     2161 tgctcattac agccctgtag ataaacgaga tattcgcggt agctttcggt tttggcgtgc
     2221 ttctggcagc cgatagccat atacaattca gcagtggttt tacctagcag aatttcccgt
     2281 agccactcga ccagcgctgc gggcagcggt gcataaacca taaaataaga acgcgcaaag
     2341 ccaaaaacaa tgctcgcttc ggcggtcgtc gtcaggcagg tatcaataaa taactcgccg
     2401 tcgtccgtct ggtggatcgg cagtaagaag ggcaacgtgc cggaaggcgt gatcagtttg
     2461 cccaccagcc aggcggcttt attgcggtaa aacagttcgt tcgccacctg taaatgactt
     2521 tccgcgaggt tgtctgtccc cagcgtttcc gtcagatggc gaatgatgta atgaatgtca
     2581 cggcttttat tctgccagcg caggcgcagc ggcaggtcgc tgataacgcg catcagtaga
     2641 gattcccagc cgtgatcggg gtgaaagtct ttcgccagcg ggcggggaat ggtacgaaag
     2701 cggcgctctg gctgagagct aaagataaaa agccgctcgg gagtaagcga gcggtggtca
     2761 aataaccgac agtacacgga gttaaaaaag ctctccgcaa tctcgaagcg cgggtaatcc
     2821 ggcaacagcc gcgtgtaatg ctctttaacg cgcagtaaaa atgccgcgtc cgtactttgg
     2881 ccgttagtaa tgcagcgcag ttgctccacg accagaccaa cgtgatgatc gtaaagatgg
     2941 atacggtttt tcatcgcctg ctggacagca tgccagtcgg cctgttcgaa acgctgctgc
     3001 gccccggaag tcacttcgag gaatcgacca tactgagcgt cgaagccttg caaaatggtt
     3061 tgagcaatca gtaattccag gccacgcggc atttagcgcc ctcatcagga gcagagaatt
     3121 gccggatgcg gcgtaaacgc cttatccagc ctacgttcgg caacggttgt aggcatgata
     3181 agacgcgcaa gcgtcgcatc aggcatcgtg caccgattgc cggatgcggc gtaaatgcct
     3241 tatccggcct acgttcggca acggctgtag gcatgataag acgcgcaagc gtcgcatcag
     3301 gcatcgtgca ccgattgccg gatgcggcgt gaacgcctta tccggcctac agtcagcaac
     3361 ggttgttgtt gcttagaact gcgattcttc agtggagccg gtcagcgcgg tgactgaaga
     3421 cgtgccgccc tgaataatag tcgtcacttt atcgaagtaa cctgtaccca cttcctgctg
     3481 gtgagatacg aaggtatagc catctttcgc ggcggcaaat tccggctgct gcactttctc
     3541 aacgtagtgc ttcataccct cgccctgggc ataggcgttt gccaggtcaa acatgttgaa
     3601 ccacatgctg tggatacctg ccagggtgat gaactggaac ttgtagccca tatccgacag
     3661 ctgctgctgg aagctggcaa tagttttgtc gtcgaggttt ttctgccagt tgaacgacgg
     3721 cgagcagtta taagccagca gtttgcccgg atatttcgcg tggatagctt gtgcaaagcg
     3781 acgcgccagt tccagatccg gcgtggaggt ttcacaccag accaggtcag catatggcgc
     3841 atacgccagg ccacggctga tcgcttgctc aatgcccgca tgagtacgga agaagccttc
     3901 actggtacgc tcgccagtaa taaattcact gtcatacggg tcgcaatcgg aggtaatcag
     3961 atccgccgca tcggcatcgg tacgagcaac cagcagggtt ggaacgcccg tcacgtcagc
     4021 tgcaagacgc gccgcgacca gtttctgaat agcttcctga gttggcacca aaactttgcc
     4081 gcccatgtga ccgcatttct tcactgacgc cagctgatct tcgaagtgaa ctgccgctgc
     4141 accggcttca atcatcgctt tcatcagttc aaaggcattc aggacaccgc caaaaccggc
     4201 ttccgcatcg gcaacgatcg gcaggaagta atcgacatag cgcggatcgc ccggctcaat
     4261 gcctgcggac cattggatct gatcggcacg acggaaggtg ttgttgatcc gctccaccac
     4321 agctggcacc gagtttgccg gatagagcga ctgatccgga tacatgctgg ccgccaggtt
     4381 agcgtccgcc gctacctgcc atcccgacag atagactgct tcaatacccg ctttcgcctg
     4441 ttgcagcgcc tgaccgccag tcagtgcacc gaggctgttg atgtagcctt ttttcgactc
     4501 gccatgcagc agacgccaca ttttcgccgc gcccagttgc gccagggtgc attcaggatt
     4561 gactgaaccg cgtaatttca ccacatcttc cgcgctgtat gggcgagtaa tgccttccca
     4621 acgcggttga gtccactctt tctgtaattc ttcaatttgt tgtgtacggg ttttcatgtg
     4681 cagatgctcc atagttatgt ggtggtttac gctaacaggc ggtagcctgg cagggtcagg
     4741 aaatcaatta actcatcgga agtggtgatc tgttccatca agcgtgcggc atcgtcaaaa
     4801 cgcccctggg aaaaacgttc ttcgcccagt tcgctggcaa tgactttcat ctcttcaccc
     4861 agcatctggc ggaacaaggc tttggtcacc ggtttgccat tgctcaacgt tttttgatga
     4921 tggatccact gccagattga ggtacgggaa atttcagccg tcgccgcatc ttccatcagg
     4981 ccataaatcg gcacgcagcc gttgccggag atccatgctt cgatgtactg cactgccacg
     5041 cgaatgttgg cgcgcatacc ttcttcggtg cgttcaccat cacaaggtgc cagcagctga
     5101 tcggcagtaa tcggcgcgtc ttgttcgcgc atcacttcaa gctgattttt acgggagccg
     5161 agaatgtcgt tgaataccgc cattgccgta tccgcaaggc ctgggtgagc aatccatgtg
     5221 ccatcgtgac cgttattggc ttccagcgct ttatccgctt ttactttgtt gagcacctgg
     5281 ttattgcgct cttcatcttt gctcggaata aacgctgcca tgccgcccat cgcaaaagca
     5341 ccgcgtttat ggcaggtttt aatcaacagg catgagtaag cattcaggaa tggtttatcc
     5401 atcgtcactg cctgtctgtc tggcaggacg cgatcgggat agtttttcaa cgttttgata
     5461 tagctgaaga tgtaatccca acgaccgcag ttcagaccaa caatatggtc acgcagcgcg
     5521 tgaaggattt catccatctg gaacacggcg ggcagcgttt caatcagcaa cgtcgccttg
     5581 atggtgccgc gcggcagatt aaagcgatct tctgcatagc tgaagacttc gctccaccag
     5641 gccgcttcct gccaggactg ggttttcggc agatagaaat agggaccgct gccctttgcc
     5701 aacagtgcct gatagttgtg gaagaaatag agcgcaaaat caaacaggct accggggatt
     5761 gcctcaccac gccaggtgac atgtttttcc ggcaagtgca gaccgcgtac ccgacaaatc
     5821 aaaaccgctg gattgggctt gagctgataa attttgcctg cttcattggt atagctgatg
     5881 gtgccgttaa ccgcatcgcg caggttaatt tgcccgtcga tcactttgtt ccagtctggt
     5941 gccagtgaat cttcgaaatc ggccataaag actttcacat tggcgttcag tgcgttgatc
     6001 accatcttgc gctcaaccgg gccagttatc tcgactcgac gatcttgtaa gtccgcggga
     6061 ataccacgaa ttttccagtc cgtatcgcga atggaagcca tttccgaaat aaaatcaggc
     6121 aaagttccgt tatcaatatc ttgctgctgc tgaatgcgcg ctgccagaag tttattgcgt
     6181 tgtggcgtaa aatgcgtcac cagctcagtc agaaattcta ccgcttcggc agtaagaatt
     6241 tgcttctcct gctcgccata cggccttatg aaagccagtt catcggttgt tgttgcctgt
     6301 tcagtcatcg tgcagctcct cgtcatggat ccgaaaactt ccccactgtg aacgaaggat
     6361 cgttgtgcgc ttttcgttca gcacaactaa gactactcat ttaaaatgca aaatcaaaaa
     6421 caatttccat tttaattaaa aatacttgat aacttattga taacaatatg aataaaattt
     6481 attcacctcc tgaggtgcgt ttcggaaata aaaaaggcac ccgaaggtgc ctgaggtaag
     6541 gtgctgaatc gcttaacgat cgactatcac agaagattaa tccagcgttg gattcatgtg
     6601 ccgtagatcg tatggcgtga tctgatagac gtaatagttg agccagttgg taaacagcaa
     6661 attaccgtga ctacgccagc tcgctcgcgg tgtattttgc ggatcattgt gcgggaaata
     6721 gttatacggt acttccgggc ctagtccggc ttccacatcg cggaaatatt cctgcgccag
     6781 cgtttgcgca tcatattcgg gatggcccgt cacaaaggca atgcgcttat ctttgctggc
     6841 aaacagatat gcatcccctt cttccgtctc tgccagaatt tccagatcgg tgtaatcacg
     6901 aatcaacgct gccggaaagt cagcatagcg cgaatgtggt gccaggaatg aatcatcaaa
     6961 gccacgcgtc agaagcgcat gaggatggag aatatgatgc tcgtaaacgc cagagagttt
     7021 gtcggtgcga gtttgcttag gaatgccgta gaggatattg agcgcggcct gtaccgccca
     7081 gcagacaaac agcgtcgagg tgacgtgatc tttcgaccac tccagcacct gtttgatctg
     7141 cggccagtaa gcgacatcat taaactccac caggcccagc ggcgcaccgg ttacaatcag
     7201 accgtcaaag ttctgttcct gaatatcttc aaagttacag tagaagttgt tcagatgctc
     7261 tgcgggcgtg ttgcgcgatt cacgggaatc gatgcgcaac agctgaatat cgacctgcaa
     7321 aggtgagttt gaaagcaggc gcagaaactg attttcagtt tcaatcttct tcggcatcag
     7381 gttaaggatc agaaccttaa gtggacgaat ttcctgacca gacgcacgag aagttgtcat
     7441 cacaaagacg ttttcttcac gcaagaaatt gacggcgggt agctcgtccg gcacacgaat
     7501 aggcataacc tgattacctc actacatacg cttatacgtt tagacatcca gatagctgaa
     7561 gataaccaga aaatttgcca atgtcgagcc tgcatgttga aggtgagaaa gtttcaagga
     7621 aaaggctacg ttagaatata agaatgacga aaaggagaga aaatggttat tagtattcgc
     7681 cgctcacagc atgaggaagg ggaggaactc gttgcgattt ggtgtcgatc tgtcgatgcc
     7741 acccacgatt ttttatcagc agagtatcgg gccgagctgg aagagctggt tcgttccttt
     7801 ctgccggaag caccgttgtg ggtcgcggtt aatgaacggg atcagccggt tgggtttatg
     7861 ttgttaagtg ggcagcatat ggatgcgctg tttatcgatc ctgatgtgcg cggctgcggc
     7921 gtgggtcgga tgctggtgga gcatgcgctc tcgatggcac cggaactgac aaccaacgtt
     7981 aatgagcaaa atgagcaggc ggttgggttc tataagaagg tgggttttaa ggttacggga
     8041 cactctgagg tggacgattt ggggaaaccg tatccgttgc tgaatctggc gtatgtgggg
     8101 gaataagccg cccctgccgt gcctgcccag gctggagaag acgcgccagc gtcgcacccg
     8161 gcattttttt caccaacgca aaaaggccat cgtgactgat ggcctttcgc ttaatttgat
     8221 gcctggcagt tccctactct cgcatgggga gaccccacac taccatcggc gctacggcgt
     8281 ttcacttctg agttcggcat ggggtcaggt gggaccaccg cgctaaggcc gccaggcaaa
     8341 ttctgtttta tcagaccgct tctgcgttct gatttaatct gtatcaggct gaaaatcttc
     8401 tct
//
LOCUS       DAZGVQ010000200         8279 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000200 DAZGVQ010000000
VERSION     DAZGVQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.202"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(58..134)
                     /locus_tag="ACHLAG_004621"
     tRNA            complement(58..134)
                     /locus_tag="ACHLAG_004621"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(98..100),aa:Asp,seq:gtc)
     gene            complement(267..998)
                     /gene="dnaQ"
                     /locus_tag="ACHLAG_004622"
     CDS             complement(267..998)
                     /gene="dnaQ"
                     /locus_tag="ACHLAG_004622"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFP0657729.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1063..1530
                     /gene="rnhA"
                     /locus_tag="ACHLAG_004623"
     CDS             1063..1530
                     /gene="rnhA"
                     /locus_tag="ACHLAG_004623"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFP0657730.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHSWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1527..2249)
                     /locus_tag="ACHLAG_004624"
     CDS             complement(1527..2249)
                     /locus_tag="ACHLAG_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP0657731.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            2283..3038
                     /gene="gloB"
                     /locus_tag="ACHLAG_004625"
     CDS             2283..3038
                     /gene="gloB"
                     /locus_tag="ACHLAG_004625"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFP0657732.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSNPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3110..4468
                     /gene="mltD"
                     /locus_tag="ACHLAG_004626"
     CDS             3110..4468
                     /gene="mltD"
                     /locus_tag="ACHLAG_004626"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFP0657733.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4516..5286)
                     /locus_tag="ACHLAG_004627"
     CDS             complement(4516..5286)
                     /locus_tag="ACHLAG_004627"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP0657734.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(5364..6164)
                     /locus_tag="ACHLAG_004628"
     CDS             complement(5364..6164)
                     /locus_tag="ACHLAG_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP0657735.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGESLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6405..7319
                     /gene="yafC"
                     /locus_tag="ACHLAG_004629"
     CDS             6405..7319
                     /gene="yafC"
                     /locus_tag="ACHLAG_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFP0657736.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMASAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVAMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7316..8119)
                     /gene="dkgB"
                     /locus_tag="ACHLAG_004630"
     CDS             complement(7316..8119)
                     /gene="dkgB"
                     /locus_tag="ACHLAG_004630"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFP0657737.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAKDKKAIAALDCNDRLVSPKGLAPEWD"
BASE COUNT         2197 a         1990 c         2013 g         2079 t
ORIGIN      
        1 aaaaaccgca aaattaaaaa tcttgcggct ctctgaactc attttcatga gtgaatttgg
       61 cggaacggac gggactcgaa cccgcgaccc cctgcgtgac aggcaggtat tctaaccgac
      121 tgaactaccg ctccgcgttg tgttccgttg ggaacgaggc gaatattacg gattgcctca
      181 cccttcgtca acggtttttc tcatcttttg aatcgtttgc tgcaaaaatc gcccaagccg
      241 ctatttttag cgccttttac aggtatttat gcccgccaga ggcagcttcc gcccttcttc
      301 tccaccagat caagacgggc ttcatgagct gcaagctctt catctgtcgc aaaaacaacg
      361 cgtaacttac ttgcctgacg tacaatgcgc tgaattgttg cttcaccttg ttgctgctgt
      421 gtctctcctt ccatcgcaaa agccatcgac gtttgaccac cggtcatcgc cagataaact
      481 tccgcaagga tctgtgcatc gagtaatgcc ccgtgtagcg ttcgtttact gttatctatt
      541 tcgtagcggg cacataacgc atcgaggctg ttgcgcttac caggaaacat tttcctcgcc
      601 accgcaaggc tatcggtgac cttacagaag gtattggtct tcggaatatc gcgcttaagc
      661 aacgaaaact cgtagtccat aaagccgata tcgaacgctg cgttatggat caccaactcc
      721 gcgccgcgga tatagtccat gaactcatcg gctacttcgg caaacgtggg cttatcgagc
      781 aaaaattcat cggcaatacc atgtacgcca aaggcttccg gatccaccag ccgatcgggt
      841 ttgagataaa catggaagtt attgcccgtc aggcgacggt tcaccacttc aacggcacca
      901 atctcaatga tcttgtggcc ttcatagtgc gcaccaatct ggttcatacc ggtggtttcg
      961 gtatcgagaa cgatctggcg tgtaattgca gtgctcatag cggtcattta tgtcagactt
     1021 atcgttttac agttcgattc aattacagga agtctaccag agatgcttaa acaggtagaa
     1081 attttcaccg atggttcgtg tctgggcaat ccaggacctg ggggttacgg cgctatttta
     1141 cgctatcgcg gacgcgagaa aacctttagc gcaggctaca cccgcaccac caacaaccgt
     1201 atggagttga tggccgctat tgtcgcgctg gaggcgttaa aagaacattg cgaagtcatt
     1261 ttgagtaccg acagccagta tgtccgccag gggatcactc agtggatcca tagctggaaa
     1321 aaacgtggct ggaaaaccgc agacaaaaaa ccagtaaaaa atgtcgatct ctggcaacgt
     1381 cttgatgctg cattggggca gcatcaaatc aaatgggaat gggttaaagg ccatgccgga
     1441 cacccggaaa acgaacgctg tgatgaactg gcccgtgctg cggcaatgaa tcccacactg
     1501 gaagatacag gctatcaagt cgaagtttaa gcctgtggtt tacgatattg ccgggtggct
     1561 ccaaccgcct ggcgaattcg tggcttgttt ttactctgtt tcatcggatt tagcgttaaa
     1621 ggaatagtcc gtttccgggc aacaataagt tgtaagcaac caagcgcagg aatatgtgca
     1681 ttcaatagtt ttccaccgtg tttgttccac gggagaacgt ggaaacggct ggcgtgcagc
     1741 acttcaaaat tcaacaaaga gagccagtcc agttgccgca tcagagtaaa catccggctg
     1801 ttatagggcg aggttttgcg caataccggc acaagtttgc gtaatcccat taaactgatg
     1861 ggattgaagc cactaatcac cagccagcca tcatcaatca atacccgatc ggcttcacgc
     1921 aataaacgat gcggatcggt gcaccacggc aatgtatgtg ccagtagaca aacatcaacg
     1981 gatttatcgg caaaaggaag atgaagtggg tccgcctgta cctggacggg cattccttgc
     2041 gcagaaacat tcacttgatg agaaaccgcg cacgcttcgc aattgatttc tgcgcttaaa
     2101 ttgccaatct taagcagatg aaaaccatac attttagtga accacgggtt gagctggcgc
     2161 tccagcgcct tgcgataaag ctctccccag ggcaaatcgc cccagcaatc aggagccacg
     2221 acagtttgag ggactcttgc cggtttcatc acaaccttcc gtttcacact aagaggtaat
     2281 ctatgaatct taacagtatt cccgcctttg atgacaatta catctgggtt ttgaatgatg
     2341 aagcaggtcg ctgcctgatt gtcgatcccg gagacgcaga gccagtatta aacgccatta
     2401 ccgccaataa ctggcaaccg gaggccatat ttctcaccca ccatcatcac gatcacgttg
     2461 gcggcgtaaa agaactggtg gaaaagtttc cgcaaattgt ggtgtatggt ccacaagaga
     2521 cacaagataa gggaacaaca caggtagtca aagatggcga aactgccttc gttttggggc
     2581 atgaatttag tgtaattgcc acaccgggtc acactttagg acatatctgt tacttcagta
     2641 acccttatct attttgcggc gacacgctgt tttctggtgg gtgtggtagg ttgtttgaag
     2701 ggacagcatc acaaatgtat caatcactta ataagttaag tgcgttacct gacgatacat
     2761 tggtatgttg tgctcatgaa tatacgttat caaatatgaa gtttgctttg agtattcttc
     2821 cgcacgattt gtccataaat gattattatc gtaaagttaa ggagttacgg gcaaaaaatc
     2881 aaataacact acccgtaatt ctgaaaaatg agcggcaaat taatgttttt ttaagaacag
     2941 aagatattga tttaattaat gtaattaatg aagaaacatt attgcaacaa cctgaagaac
     3001 gttttgcatg gttaaggtca aagaaagata ggttctgata aaactttctt gtcatcagct
     3061 ccgttcgccg ttatgatcgg tcgtctttta agcaactatt gacacacaca tgaaggcaaa
     3121 agcgatatta ctcgcctctg tcctgctcgt gggttgccag agtaccggca acgttcaaca
     3181 gcacgcacag agcctttctg cagctggtca aggggaagca gcaaagttta caagtcaggc
     3241 acgatggatg gacgatggga cgtctatcgc gccagatggt gacttgtggg ctttcattgg
     3301 cgacgagcta aagatgggaa ttccggaaaa tgaccggatt cgcgaacaga aacagaaata
     3361 tttacgcaat aagagctatc tccacgatgt aactttacgg gcagagccgt atatgtactg
     3421 gatagccggg caagttaaaa aacgtaacat gcctatggaa ctggtactac tacccatagt
     3481 ggagagcgct tttgatcctc acgcaacgtc tggcgccaat gccgcaggca tctggcagat
     3541 cattccgagc acggggcgca attatggttt gaaacagacc cgcaattatg acgcgcgtcg
     3601 cgatgttgtt gcttcaacaa ctgccgcgct gaacatgatg cagcgtctga acaagatgtt
     3661 tgacggcgac tggcttctga ccgtagcggc ttataacagc ggcgaaggtc gggtcatgaa
     3721 ggcaattaaa acgaacaaag cgcgtgggaa atccacggac ttctggtcgt taccgttgcc
     3781 gcaggaaacg aagcagtacg tgcctaaaat gctggcattg agtgatattc tcaaaaacag
     3841 caagcgttat ggcgtacgtc tgccaacgac cgatgaaagc cgtgctctgg cgcgtgtgca
     3901 cctgagcagc ccggttgaaa tggcgaaggt tgcagatatg gcggggattt ccgtcagcaa
     3961 gctgaagaca ttcaacgctg gcgtgaaagg ctccacgctg ggcgcaagtg gtccgcaata
     4021 cgtgatggtg ccaaagaagc atgcagatca actgcgtgaa tctctcgctt caggcgaaat
     4081 tgctgctgta cagtcgacgc tggttgccga caatacgccg cttaacagcc gtgtttacac
     4141 cgtacgctct ggcgacacgc tttcaagtat cgcttcacgt ctcggcgtaa gcaccaaaga
     4201 tttgcagcag tggaacaaac tgcgcggatc taagctgaag ccaggccaaa gtttgacgat
     4261 tggtgcaggc agtagcgcac agcgactggc aaacaacagc gatagcatta cgtatcgtgt
     4321 gcgcaaaggc gattcgcttt caagcattgc taaacgccac ggcgtgaaca tcaaagatgt
     4381 gatgcgctgg aacagcgaca ctgcgaatct gcaaccaggc gacaagctga cgttgtttgt
     4441 gaaaaacaac aacatgccag attcctgaca aaccagataa taaaaaggca ccgattcccc
     4501 cggtgccttt tttatttatg ctgctttatg tgcttctacc atgatgatat cactggtgaa
     4561 agagccatca ttctgcaagg caaaatacgt tctcacctct gtcgatgcgc tctgttggta
     4621 aatgcgaata gcgtctacta acgcttctgg cgtacgcatt ctcgcgaccc atgaagaaaa
     4681 ttccagcggt aacttatctg taattaaatt atcaactatc agattggctt cattgattaa
     4741 cctcaaccac tcaccgctgg cgtagtttcg tacgtgagag gtatcgcgta atgcttctac
     4801 cgtctgtaac cagatgtcgc gcactggatg acccggagac attacgtcca tcacaatcat
     4861 cctaccgcca ggtttcaata tcctattcac ttctcgcagt gctgcaccaa catcatgcca
     4921 gtgatgggca gaataacggc tgataacaat atcaaatgcg ttatcggcaa atggcagact
     4981 ttcggcataa ccctggcggg tggtgatatt tttcagttgc cgggcttcgg cagcttgtgc
     5041 cacgacatcc agcatttggg cagataagtc atacgccacc accgcgctca cgttctgcgc
     5101 agcgacaaag ctggcatgcc ctgctccgca gcccatatca agtacacttg catcaggata
     5161 atcagccaga cgcaccgcca ggcgctgtaa atctcgcccg gatgcatgta cggtactggt
     5221 taaatactca ctggcctgcg agctaaactg cttttctacg tggtcatggt gggattgtgt
     5281 tgtcattgcg ctgtcctttt gttgtttgag tataaaagga cagcgccaaa cgttggtctg
     5341 ccctgtggca gacctgacat accttattta tcaggcttgc cgggactgaa ttcaacgagt
     5401 agcggattgt gatcggaagc gcgcgtaacc aatacagaag cttcgctgac gttcagacca
     5461 cggtagaaaa caaaatcgag cgggcggcca aacgcccgac ggcgctgatc atcggtaaaa
     5521 cgcacctggc gcagcgacat ttcccgcgca aagcgatata acgcgttcat ccttctacgg
     5581 ctccaggcat tgaaatctcc tgccataatg accgggccgc tgtggtgagc tatctgatca
     5641 ccaataggaa gtaactgctt actatagaca tccacgccca gactgaagtt gacggcgtgt
     5701 atattaacca ccatcaacag gcgggtgtca ggtaatggat agaccgtcac cagtgccgac
     5761 ttcgccagac gcaaaatggg ttctcgttca cgtaacgggc agcaatacac tggatgcgcc
     5821 gccgaaaggg tcattacgcc agaaggatgt tgtggcagta cgaaagcggg tacctgatcg
     5881 gcggcaagat agttagcggt cgcaaactgt actaactctg gcgttgtctg cgcttcctgc
     5941 aataacacca gatgtgcatc tttgccgtag ttctttaata ccgacaacca ttcagcgcgt
     6001 tgctgtttgt atatgttcca caccaggatc cgaatacgct cttcggtact taacgattct
     6061 ccgggtggta atgcctggcc gatgctcgca aaagaccccg gcggtaagat cctttccgca
     6121 ggttgtccgg caacatagcg catggcatag gtgttttttc gcacttttga ctttgaaacc
     6181 tctattacgt caacccttcc gcactcggga gggttctact gttataggga ttttagctca
     6241 ctctttcaac gagcaattgt atattgttat gtaagcaagt gcttacaccg gtcagaattt
     6301 tgctcattat ggagaacatt aaatcgcatg acatatcacg catcctgatg gtttaatgac
     6361 aaataaattc aaaatatctc ttctattttg caacaggagc aaaaatgaaa gccacgtcgg
     6421 aagaactcgc catttttgtt tcggtcgtcg aaagcggcag ctttagccgg gcagcggaac
     6481 aattagggca agcaaactca gcggtaagcc gggcggtgaa aaagctggag atgaaacttg
     6541 gcgttagcct gcttaatcgg accacgcgac aacttagcct gacggaagaa ggcgagcgtt
     6601 attttcgtcg cgtacagtca attttgcagg agatggcatc ggcagaatca gaaattatgg
     6661 agacgcgtaa tacaccgcgt ggactgttac ggatcgatgc cgcaactcca gtggtgctgc
     6721 actttctgat gccgttaatt aagcctttcc gtgaacgcta tccggaagtc actttgtcgc
     6781 tagtctcctc cgaaacgatt attaatttga tcgaaagaaa agtggatgtc gcgatacgcg
     6841 ctggtacgtt aacggattcc agcttacgtg ccaggccgtt atttaacagt tatcgaaaaa
     6901 ttatcgcctc ccccgattat atttcccgct acgggaagcc agaaacgatc gacgatttaa
     6961 agcaacatgt ttgcctggga ttcactgaac ccgcttccct caatacctgg ccgatagcct
     7021 gtagtgatgg acaattacat gaggtgaagt acggtttgtc atccaatagt ggggaaacac
     7081 tgaaacagct ttgcctgagt ggcaacggga ttgcgtgttt gtccgactac atgatcgaca
     7141 aagaaatcgc tcgcggagaa ttggtggagt taatggcaga taaagtgttg ccagtggcaa
     7201 tgccattcag tgcagtctat tacagcgacc gtgcggtaag tacgcgcatc cgggctttta
     7261 tcgatttcct tagcgagcat gtaaaaacag ctcccggagg agctgtcaga gaggcttaat
     7321 cccattcagg agccagacct ttcgggctaa ccaggcggtc gttgcaatcc agggcggcga
     7381 tcgctttttt atctttggca tcgagctgta aattttgtgc cttaagatta ctttccaggt
     7441 ttttacgttt agtagaagaa ggaattactg agtaaccttc ccccatagcc cacgccagaa
     7501 tcacttgtgc cggagtcgca ttgtgtttag ctgcgatacg agcaataacc tcatctttca
     7561 gggccttacc atacgccagc gtcatatagg aagtaatatg gatgccgtgc tgtttagccc
     7621 aggcaaccac tttacggttt tgcagataag gagagagttc aatctggtta gtagcgatgt
     7681 tttcagcgcc aacagcagca atcgcctttt ccatcaatgg gatcgtgaag ttggaaatac
     7741 cgatctcacg cgtcagccct tgttttttgg cttccagcag cgcctgcata aactcttcaa
     7801 cagagacttc atcgtttggt gacggccagt ggattagcgt cagatcaaca taatcggtac
     7861 gcaatttttg caggctctct ttcagacttg ggatcaattt gtctttgctg agattttcaa
     7921 tccagatttt agtggtgatg tagagttcat gacgtggcac gccactttct gcaatcgcct
     7981 gacctactgc ggcttcgtta tcatagattt gtgcggtatc aattgcgcga taaccaagtt
     8041 caagcgccgt tttcacagat gaaataacaa cgtcgtcttt cagacggaaa gtacctaaac
     8101 caaatgcagg gatagccata atatgcctct tttaatgcta ttttcgttaa ctgaggtgat
     8161 tatgggcttc agactgttga ggaaaaagag cgtaaaatgc agaggatttt tgcgattctg
     8221 gcaataatag atatacagac gaaaaaaaac ctcgagcgtt aactcgaggc ttcttaata
//
LOCUS       DAZGVQ010000201         8270 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000201 DAZGVQ010000000
VERSION     DAZGVQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8270
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.203"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(124..744)
                     /gene="sodA"
                     /locus_tag="ACHLAG_004631"
     CDS             complement(124..744)
                     /gene="sodA"
                     /locus_tag="ACHLAG_004631"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFP0657738.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            1029..2063
                     /gene="rhaT"
                     /locus_tag="ACHLAG_004632"
                     /pseudo
     CDS             1029..2063
                     /gene="rhaT"
                     /locus_tag="ACHLAG_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(2060..2908)
                     /gene="rhaR"
                     /locus_tag="ACHLAG_004633"
     CDS             complement(2060..2908)
                     /gene="rhaR"
                     /locus_tag="ACHLAG_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFP0657739.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(2982..3818)
                     /gene="rhaS"
                     /locus_tag="ACHLAG_004634"
     CDS             complement(2982..3818)
                     /gene="rhaS"
                     /locus_tag="ACHLAG_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFP0657740.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLVWLEDHFADEVNWDAVAEQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            4106..5575
                     /gene="rhaB"
                     /locus_tag="ACHLAG_004635"
     CDS             4106..5575
                     /gene="rhaB"
                     /locus_tag="ACHLAG_004635"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFP0657741.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNMVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASALIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNALLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFIPNPDSEIAHYLAQIHSTRQTKELCA"
     gene            5572..6831
                     /gene="rhaA"
                     /locus_tag="ACHLAG_004636"
     CDS             5572..6831
                     /gene="rhaA"
                     /locus_tag="ACHLAG_004636"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFP0657742.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPTGSEWLESVRAYEKAILSQRG"
     gene            7073..7897
                     /gene="rhaD"
                     /locus_tag="ACHLAG_004637"
     CDS             7073..7897
                     /gene="rhaD"
                     /locus_tag="ACHLAG_004637"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFP0657743.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            7907..8221
                     /gene="rhaM"
                     /locus_tag="ACHLAG_004638"
     CDS             7907..8221
                     /gene="rhaM"
                     /locus_tag="ACHLAG_004638"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFP0657744.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         1956 a         2153 c         2214 g         1947 t
ORIGIN      
        1 atttttatcg gatcattgtt tttattttga aattgatcac aaaaaaacca ccgccgttgg
       61 cgatggttca ttttttaagc tgatatgcgg cctatacgcc tcattgcagc aggcggcaaa
      121 tgattatttt ttcgccgcga aacgcgccgc agcttcgtcc cagttcacca cgttccagaa
      181 ctctttgatg tagtccgggc ggcggttctg gaatttcagg tagtaagcat gttcccacac
      241 atccaggccc agaatcggga agccggaagc gccagaaata gcttcaccca tcagcggaga
      301 atcctggtta gcagtagaaa ccaccgccag tttatcgcct ttcagcacca gccatgccca
      361 gccggaacca aagcgggaag ctgccgcttt ttcaaattct gctttgaagt tatcaacgga
      421 gccgaagtca cgttcgatag ccgctttcag gtcaccctgc agggtggtgc cttttttcag
      481 acctttccag aacaggctgt ggttagcgtg accgccagcg ttgttgcgca gtacggtttt
      541 cttgtctgct ggcagctggt ccagtttggt gatcagctct tcaaccggca ggttggcaaa
      601 ttctggcagg ctttccagcg ccgcgttggc gttgtttacg taggtctgat ggtgtttggt
      661 gtggtggatt tccatggtct gcttatcgaa gtgcggttcc agggcatcgt aagcatacgg
      721 cagggatggc agggtatagc tcatattcat ctccagtatt gtcgggcggc cgattgttaa
      781 tgccgcgtaa gcagttggtt cattatagtt aattaaatga tattgaaaat gattatcaat
      841 gccgtacttt tcgtaagggt atggttttgc aggaaaatgc ccgagatgtg aagcaaatca
      901 cccacttaat gccgtgattg ccagtaaatc gacaacggcg gcaacaggcg aaaggttaat
      961 cgacagcacg atttttacac tcatctcgtc ggagatgtga agcgacgaaa aatgatgagg
     1021 ataagaagat gagtaacgcg attacgatgg ggatattttg gcatttgatc ggcgcggcca
     1081 gtgcagcctg tttttacgct ccgttcaaaa aagtaaaaaa atggtcatgg gaaaccatgt
     1141 ggtcagtcgg tgggattgtt tcgtggatta ttctgccgtg ggccatcagc gccctgttac
     1201 taccggattt ctgggcgtat tacagctcgt ttagtctctc tacgctgctg cctgtttttc
     1261 tgttcggcgc tatgtggggg atcggtaata tcaactatgg cctgaccatg cgttatctcg
     1321 gcctgtcgat gggaattggc atcgccattg gcattacgtt gattgtcggt acgctgatga
     1381 cgccaattat caacggcaat ttcgatgtgc tgattaacac cgaaggcgga cgcatgacgt
     1441 tgctcggcgt tctggtggcg ctgattggcg tagggattgt gactcgcgcc gggcagttga
     1501 aagagcgcaa aatgggcatt aaagccgaag agttcaacct gaaaaaaggg ctggtgctgg
     1561 cggtgatgtg cggcattttc tctgccggta tgtcttttgc gatgaacgcc gcaaaaccga
     1621 tgcatgaagc cgctgccgca ctgggcgtcg atccactgta tgtcgctctg ccaagctatg
     1681 tcatcatcat gggcggcggc gcgatcatca acctcggttt ctgcttcatt cgtctggcaa
     1741 aagtgaagga tttgtcgcta aaagccgact tctcgctggc aaaaccgctg atcattcaca
     1801 atgtgttact ctcagcactg ggcgggttga tgtggtatct gcaattcttt ttctatgcct
     1861 agggccacgc ccgcattccg gcgcagtatg actacatcag ttggatgctg catatgagtt
     1921 tctatgtatt gtgcggcggt atcgtcgggc tggtgctgaa agagtggaac aatgcaggtc
     1981 gccgtccggt aacggtgttg agcctcggtt gtgtggtgat tattgtcgcc gccaacatcg
     2041 tcggcatcgg catggcgaat taatctttct gcgaattgag atgacgccac tggctgggcg
     2101 tcatcccggt ttcccgggta aacaccaccg aaaaatagtt actatcttca aagccacatt
     2161 cggtcgaaat atcactgatt aacaggcggc tatgctggag aagatattgc gcatgacaca
     2221 ctctgacctg ccgcagatat tgattgatgg tcattccagt ctgctggcga aattgctgac
     2281 gcaaaacgcg ctcactgcac gatgcctcat cacaaaattt atccagcgca aagggacttt
     2341 tcaggctagc cgccagccgg gtaatcagct tatccagcaa cgtttcgctg gatgttggcg
     2401 gcaacgaatc actggtgtaa cgatggcgat tcagcaacat caccaactgc ccgaacagca
     2461 actcagccat ttcgttagca aacgacacat gctgactact ttcatgctca agctgaccga
     2521 taacctgccg cgcctgcgcc atccccacgc tacctaagcg ccagtgtggt tgccctgcgc
     2581 tggcgctaaa tcccggaatc gctccctgcc agtcaagatt cagcttcagc cgctccgggc
     2641 aataaataat attctgcaaa accagatcgt taacggaagc gtaggagtgt ttatcgtcag
     2701 catgaatgta aaagagatcg ccacgggtaa tgcgataagg gcgatcgttg agtacatgca
     2761 gaccattacc gcgccagaca atcaccagct cacaaaaatc atgtgtatgt tcggcaaaaa
     2821 catcttgcgg ataacggtca gccacagcga ctgcctgctg gtcgctggca aaaaaatcat
     2881 ctttgagaag ttttaactga tgcgccaccg tggctacctc ggccagagaa cgaagttgat
     2941 tattcgcaat atggcgtaca aatacgttga gaagattcgc gttattgcag aaaaccatcc
     3001 cgtccctgac gaatatcacg cggtgaccag ttaaactctc ggcgaaaaag cgtcgaaaag
     3061 tggttactgt cgctgaatcc acagcgatag gcgatatcag taacgctggc ctcgctgtgg
     3121 cgtagtagat gtcgggcttt catcagtcgc aggcggttca ggtaacgctg aggcgtcagt
     3181 cccgtttgct gcttaagctg ccgatgtagc gtacgcagcg aaagagaaaa ttgctccgcc
     3241 acggcatccc aattcacctc atcggcaaaa tggtcctcca gccagaccag aagcaagttg
     3301 agacgtgatg cgctgttttc caggttctcc tgcaaactgc ttttacgcag caagagcagt
     3361 aattgcataa acaagatctc gcgactggcg gtcgagggta aatcattttc cccttcctgc
     3421 tgttccatct gtgcaaccag ctgtcgcacc tgctgcaata cgctgtggtt aacgcgccag
     3481 tgggaaggat actgcccatc cagctcttgt ggcagcaact gattcagccc ggcgagaaac
     3541 tgaaatcgat ccggcgagcg atacagcaca ttagtcagac acagattatc ggtatgttca
     3601 tacagatgcc gatcatggtc gcgcacaaaa cagaccgtgc caccggtgat ggtatagggc
     3661 tgcccattaa acacatgaat acccgtgcca tgttcgacaa tcacaatttc atgaaaatca
     3721 tgatgatgtt caggaaaatc cgcctgcggg agccggggtt ctatcgccac ggacgcgtta
     3781 ccagacggaa aaaaatccac actatgtaat acggtcatac tggcctcctg atgtcgtcaa
     3841 cacggcgaaa tagtaatcac gaggtcaggt tcttacctta aattttcgac ggaaaaccac
     3901 gtaaaaaacg tcgatttttc aagatacagc gtgaattttc aggaaatgcg gtgagcatca
     3961 catcaccaca attcagcaaa ttgtgaacat catcacgttc atctttccct ggttgccaat
     4021 ggcccatttt cctgtcagta acgagaaggt cgcgaattca ggcgcttttt agactggtcg
     4081 taatgaaatt cagcaggatc acattatgac ctttcgcaat tgtgtcgccg tcgatctcgg
     4141 cgcatccagt gggcgcgtga tgctggcgcg ttacgagcgt gaatgccgca gcctgacgct
     4201 gcgcgaaatc catcgtttta acaatgggct gcatagtcag aacggctatg tcacctggga
     4261 tgtggatagc ctggaaagtg ccattcgcct tggattaaac atggtgtgtg aggaagggat
     4321 tcgtatcgat agcattggga ttgatacctg gggcgtggac tttgtgctgc tcgaccaaca
     4381 gggtcagcgt gtgggcctgc ccgttgctta tcgcgatagc cgcaccaatg gcctaatggc
     4441 gcaggcacag caacaactcg gcaaacgcga tatttatcaa cgtagcggca tccagtttct
     4501 gcccttcaat acgctttatc agttgcgtgc gctgacggag caacaacctg aacttattcc
     4561 acacattgct cacgctctgc tgatgccgga ttacttcagt tatcgcctga ccggcaagat
     4621 gaactgggag tacaccaatg ccaccaccac acaactggtc aatatcaata gcgacgactg
     4681 ggacgagtcg ctactggcgt ggagcggggc caacaaagcc tggtttggtc gcccgacgca
     4741 tccgggtaat gtcattgggc actggatttg cccgcagggt aatgagattc cggtggtcgc
     4801 cgttgccagc catgataccg ccagcgcgct tatcgcctcg ccgttaaacg gttcacgcgc
     4861 cgcttatctc tcttctggca cctggtcatt gatgggcttc gaaagccaga cgccatttac
     4921 caatgacacg gcgctggcag ccaacatcac caatgaaggc ggggcggaag gtcgctatcg
     4981 ggtgctgaaa aatattatgg gcttatggct gcttcagcga gtgcttcagg agcggcaaat
     5041 caacgatctc ccggcgctta tcgccgcgac acaggcactt ccggcctgcc gcttcatcat
     5101 caatcccaat gacgatcgct ttattaatcc tgacgagatg tgcagcgaaa ttcaggctgc
     5161 gtgtcgggaa acggcgcaac cgatcccggg aagtgatgct gaactggcgc gctgtatttt
     5221 cgacagtctg gcgctgctgt atgccgatgt gttgcatgag ctggcgcagc tgcgtggtga
     5281 agatttctcg caactgcata ttgtcggcgg tggctgccag aacgcgctgc tcaaccagtt
     5341 atgtgccgat gcctgcggta ttcgggtgat cgccgggcca gttgaagcct cgacgctcgg
     5401 caatatcggc atccagttaa tgacgctgga tgaactcaac aatgtggatg atttccgtca
     5461 ggtcgtcagc accaccgcga atctgaccac ctttatccct aatcctgaca gtgaaattgc
     5521 ccactatttg gcgcagattc actctacacg acagacaaag gagctttgcg catgaccact
     5581 caactggaac aggcctggga gctggcgaaa cagcgtttcg cggcggtggg gattgatgtc
     5641 gaggaggcgc tgcgccaact tgatcgttta cccgtttcaa tgcactgctg gcagggcgat
     5701 gatgtttccg gttttgaaaa cccggaaggt tcgctgaccg gtgggattca agctactggt
     5761 aattatccgg gcaaagcgcg taatgccagt gagctacgta ccgatctgga acaggctatg
     5821 cggctgattc cggggccgaa acggcttaat ttacatgcca tctatctgga atcagacacg
     5881 ccagtctcgc gcgaccagat caaacctgag cacttcaaaa actgggttga atgggcgaaa
     5941 gccaatcagc tcggtctgga ttttaacccc tcctgttttt cgcatccgct aagcgccgat
     6001 ggctttacgc tttcccatgc cgacgacagc attcgccagt tctggattga tcactgcaaa
     6061 gccagccgtc gcgtttcggc ctattttggc gagcaactcg gcacaccatc ggtgatgaac
     6121 atctggatcc cggatggtat gaaagatatc accgttgacc gtctcgcccc gcgtcagcgt
     6181 ctgctggcag cactggatga ggtgatcagc gagaagctaa accctgcgca ccatatcgac
     6241 gccgttgaga gcaaattgtt tggcattggc gcggagagct acacggttgg ctccaatgag
     6301 ttttacctgg ggtatgccac cagccgccag acggcgctgt gcctggacgc cgggcacttc
     6361 cacccgactg aagtgatttc cgacaagatt tccgccgcca tgctgtatgt gccgcagttg
     6421 ctgctgcacg tcagtcgtcc ggttcgctgg gacagcgatc acgtagtgct gctggatgat
     6481 gaaacccagg caattgccag tgagattgtg cgtcacgatc tgtttgaccg ggtgcatatc
     6541 ggccttgact tcttcgatgc ctctatcaac cgcattgccg cgtgggtcat tggtacacgc
     6601 aatatgaaaa aagccctgct gcgtgcgttg ctggaaccta ccgccgagct gcgcaagttg
     6661 gaagcggcgg gcgattacac tgcgcgtctg gcactgctgg aagagcagaa atcgttgccg
     6721 tggcaggcgg tctgggaaat gtattgccaa cgtcacgata ccccaacggg tagcgaatgg
     6781 ctggagagcg tgcgggctta tgagaaagcg attttgagcc agcgtgggta aacactgccg
     6841 gatgcggcac gagcgcctta tccggcctac gggtcgaatg tccatgcggg ctgtcaccct
     6901 caccccggcc ctctccctga aagggcgagg gagaaaaaca tagcctcgtg cggcaacgga
     6961 gctgaacact gctccggaca gtcccctcgc ccctacgggg agagggttag ggtgaaggga
     7021 acaagcagca ccatgtctaa acaaggcaac ctaatttaca ggaacacaga acatgcaaaa
     7081 cattacccag tcctggtttg tccagggaat gatcaaagcc accaccgatg cctggctgaa
     7141 aggctgggac gagcgcaacg gcggtaacct gacgctgcgc ctggatgacg ccgatatcgc
     7201 gccttataaa gacaacttcc atgcacaacc gcgttatatc ccgctcagcc aacccatgcc
     7261 tttactggca aatacaccgt ttattgtcac cggctccggc aaattcttcc gtaacgtcca
     7321 gcttgatcct gcagctaact taggcgtcgt aaaagtcgac agcgacggcg cgggctacca
     7381 tattctctgg gggttaacca acgaagccgt tccaacttct gaacttccgg ctcactttct
     7441 ctcccactgc gagcgcatta aagccaccaa cggtaaagat cgggtgatca tgcactgcca
     7501 cgccaccaac ctgatcgccc tcacctatgt actggaaaac gacaccgcgg tctttactcg
     7561 ccaactgtgg gaaggcagca ccgagtgtct ggtggtattc ccggatggcg ttggcattct
     7621 gccgtggatg gtgcccggca cggacgaaat cggccaggcg accgcacaag agatgcaaaa
     7681 acattcgctg gtgttgtggc ccttccacgg cgtcttcggc agcgggtcga cgttggatga
     7741 aacattcggt ttaatcgaca ccgcagaaaa atcagcacaa gtattagtga aggtttattc
     7801 gatgggcggc atgaaacaga ccatcagccg tgaagagttg atagcgctcg gcaagcgctt
     7861 cggcgttaca ccactagcca gtgcgctggc gctgtaagga gcaaacatga tccgcaaagc
     7921 ctttgtcatg caggttaacc ccgacgctca cgaagagtat cagcgtcggc ataaccccat
     7981 ctggccggaa ctggaagcag tgttgaaatc tcacggtgcg cataactacg ccatttacct
     8041 cgacaaagcg cgtaatctgc tgtttgccac ggtagagatt gaatctgaag agcgctggaa
     8101 tgcggttgcc agtactgatg tttgccaacg ttggtggaaa tatatgaccg acgtcatgcc
     8161 cgcgaacccg gataacagcc cggtgagtag cgaactgcaa gaagtgtttt acctgccgta
     8221 attgcctaat gtgccggaga cgcctccggc acaatatttt taatttcgat
//
LOCUS       DAZGVQ010000202         8133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000202 DAZGVQ010000000
VERSION     DAZGVQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.204"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            26..829
                     /locus_tag="ACHLAG_004639"
                     /pseudo
     CDS             26..829
                     /locus_tag="ACHLAG_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(833..2224)
                     /gene="tcyP"
                     /locus_tag="ACHLAG_004640"
     CDS             complement(833..2224)
                     /gene="tcyP"
                     /locus_tag="ACHLAG_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFP0657745.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(2357..2947)
                     /gene="ydjM"
                     /locus_tag="ACHLAG_004641"
     CDS             complement(2357..2947)
                     /gene="ydjM"
                     /locus_tag="ACHLAG_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP0657746.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(3110..3778)
                     /gene="hxpB"
                     /locus_tag="ACHLAG_004642"
     CDS             complement(3110..3778)
                     /gene="hxpB"
                     /locus_tag="ACHLAG_004642"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFP0657747.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3925..4461
                     /gene="yniB"
                     /locus_tag="ACHLAG_004643"
     CDS             3925..4461
                     /gene="yniB"
                     /locus_tag="ACHLAG_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFP0657748.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4502..5362)
                     /gene="yniA"
                     /locus_tag="ACHLAG_004644"
     CDS             complement(4502..5362)
                     /gene="yniA"
                     /locus_tag="ACHLAG_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFP0657749.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDTIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5468..5758)
                     /gene="ghoS"
                     /locus_tag="ACHLAG_004645"
     CDS             complement(5468..5758)
                     /gene="ghoS"
                     /locus_tag="ACHLAG_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP0657750.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5859..6788)
                     /gene="pfkB"
                     /locus_tag="ACHLAG_004646"
     CDS             complement(5859..6788)
                     /gene="pfkB"
                     /locus_tag="ACHLAG_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFP0657751.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            7075..7833
                     /gene="ydiY"
                     /locus_tag="ACHLAG_004647"
     CDS             7075..7833
                     /gene="ydiY"
                     /locus_tag="ACHLAG_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFP0657752.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVSY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7886..7993)
                     /gene="yniD"
                     /locus_tag="ACHLAG_004648"
     CDS             complement(7886..7993)
                     /gene="yniD"
                     /locus_tag="ACHLAG_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFP0657753.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
BASE COUNT         2143 a         1942 c         1951 g         2097 t
ORIGIN      
        1 ttttgttaac aatatggggc gggagatgtt ttctacaaac ttaatacaga gtaattatgg
       61 tgatttaaat attaaaagcc tggctttcga ctcttttaaa gaaaggcttg agtctaccat
      121 ggcggcactc actttcttta tatcaacggg tcaatgtgac tgcgatgaaa tagcagagtc
      181 gaacttcaat cgtatgattg cctatatgtc taacattaac tatgatgcct caaagccagg
      241 agcgccagca cttagttttg atacgtatct gcaggacaat gttaaatata gagtgataat
      301 taataatctt tatggcagtg agtttcggat aagagatatt aatgagaatt ttgttgggat
      361 ggattttacc tgtgaattct gacccgaaaa aatgacgaat ttgatcaata taaaaatagg
      421 ttggttattc attatttaag gactatatac tatgaaaaaa attatataca tcctgccggg
      481 agcatttttg ctgcgattca aaaaaaatga gagtctccta aagtttcctt cgataatatc
      541 aatgcttaat atcaatcttc tttttaatcc actaaatctt ccgggaatgg gaagtggtgt
      601 gcttgaagat attatgagta ttccagatag ttcacttaga aaacgtctcg ggtatgaggt
      661 tttaagcttc tcactacagg cacattcatt gagccaggaa tgtattgata agttggacat
      721 ttttttcgct gacgatctgt ttaaatatga gtcagtatgc atcgccgcga tggaacattt
      781 aaaaagtaaa gcaacggccc ctatacaaaa cggaccgttg ccagcataag aattaacggt
      841 gtgccagttc ggcgtcgtct tcgctatcca gaatggcttt atcggtttgc ttcagccact
      901 ggctggtcag cgtgccagct gtcatcgagc cactaacgtt tagcgccgta cggcccatgt
      961 cgataagcgg ttcaacggag attaacagcg ccaccagggt tactggcagg cccatcgcag
     1021 gcagcacaat cagtgcggcg aaagttgcac caccaccgac accggcaacg cctgcggaac
     1081 taacggtaac aataccgacc agcgtcgcaa tccacatcgg gtccagcggg ttaatgccaa
     1141 ccgtaggcgc aaccatcacc gccagcattg ccggatacaa accggcgcag ccgttctgac
     1201 caatggttgc accgaaagag gcggcgaaac tggcgatgga ttcaggaacg cccagacgac
     1261 gcgtttgtgc ttccacattc agtgggatag acgcagcact ggaacggctg gtaaaggcaa
     1321 acgtcagcac aggccatacc ttacggaagt acttcagcgg actcacgcca ttaatgccca
     1381 gcagaatgcc atgcactgca aacataatca gcagaccgag gtaggacgcg acaacgaaac
     1441 ttcccagttt gatgatgtct tgcaagttag aacctgcaac cactttggtc attagagcca
     1501 gaacgccgta aggggtcaat tgcatgacaa ggcgaaccag tttcatcacc cagctttgta
     1561 gggtatcgat agcggctaag acgcgttcac ctttcggcgc atcatccttc agcagtttca
     1621 atgcagctac gccgaggaat gcggcaaaaa ttaccacgct gataattgac gtcggattgg
     1681 ctccggtaag atcggcaaac gggtttttcg ggataaagga caagaccagc tgcggaacgc
     1741 tcagatcaga gactttacca acatagttac tttcaatggc gttcagacgt gcagtttctg
     1801 caccaccctg aaccagacct tcagccgtca aaccaaacag gttggtgacc agcacaccga
     1861 ccagcgccgc aatcagcgtg gtaaacaaaa gcgtaccgat ggtcagaaaa ctgattttgc
     1921 ctaactgaga tgcgttatgc agacgggcaa ccgcgctcag aatagaggcg aagactaacg
     1981 gcataacgat catttgcagc agttgaacat agccgttacc aacgatgtta aaccactgta
     2041 cagaatcttt aagtacctgg ctgtcagaac cataaatggt atgcagggca aggccaaaaa
     2101 ccacacccat caccagaccc accagcactt ttttcgccag actccactgt ttatggcggg
     2161 tctgagccag cgcaaacagc agtacaacga acaccacgat gttcgcaatt aatggaaagt
     2221 tcatccccgt tctcctgatc ttattatttc tatcactgcg tagcagtcat agtgttggcg
     2281 caagattaac agaactgtga gctggctctt atattcaaat ggaattgctt attccagaaa
     2341 ggtgattttt gtctttttag tattcaacct gatgcttaat aagccgatga aactggatct
     2401 gcaaggtatt gatcatttgc gatgaccaac gaacagcgct gttctcgggt aatgaatggg
     2461 gcatccatac cgaccagaca aataatgcca tgcagataaa acgttccagt tggttgccct
     2521 tttggggaac caggataggc aagcggaaac gccagcggca tggccagagc aggggaacac
     2581 cggcgggtgt cagcatatcg gcaagtatgt ggctcaaata acccagcacc attccttgta
     2641 gcgcatcagc cggaatgaac cagccttccg gaaccttaag gtaaaaggtt gccagcagcg
     2701 caaataccgc cagcagactg tgggtaaaac cacgatgccc aaaagcgcgg gcgatcggtt
     2761 ttgatatcca ttttaatcgc tgcccaagaa acgactttgg gtgatcgatg tccggtaaca
     2821 aacacgtcag gattgcggaa gggacaatat gccaccagtc accctgtgcc agcacgggcg
     2881 tcagctcggc atttttggca aataccgcac aagcaataga aaagagaagg tgaccttccg
     2941 ccgtcatgat aaaactcact aaactgtcga tacgtacagt ataggatttt tatacagtgc
     3001 acggaagtgg tgacggtgta actctttctc ttaaattgac gattaatccg tcagttgaat
     3061 gcaaattcag ataaagaacc aggggcagcg agatcactgc ccctggtcat taaccgagaa
     3121 ggtcttttgc ggtgagttct gtcagcgatg agagtttgac gtttgctaat acaaaacgtg
     3181 gatcattttg cgcttctggc gcaggaacga caatggaacg catgcgggct gctttagagg
     3241 cgatcatgcc atttactgaa tcttccagcg ctacgcaggt cagtgggtca acgcccagtt
     3301 ttgctgcgca gtcgagatat acttgcggat gcggcttgct gtaaggcagt ttttcagccg
     3361 aggcgagggc atcgaaactg tcgcgtaagt caaacatggt caacactttt tccagcatat
     3421 gtagtggtga cgcggaggcc agtcccacca ataaaccttg ttctttgcat aacgcaacgg
     3481 cttcgcgcac gcctggtaat aatggacgtg tctcttcaac cagtgaaatg gcacgggcaa
     3541 taacccgttc tactacttcc tgacggcttg gcccattcca cggttgccgg gcgtaccaaa
     3601 gatcgaccac catatcgatg cgtaaaccta aggtgtccgg cagctcgtta cgacgggaga
     3661 tatccacccc caggcttgcc atcacatcca gttcggctcg atcccataaa ggttctgagt
     3721 cgataagtaa tccatccata tcaaaaattg cagcaagaat ctgacgcggg gttgacatta
     3781 caacctctcc tttagagcat tatgtgtaag gtattaagcc gtgcttatga ttaagcgcct
     3841 atcatatcgc ctttaaagaa tgggcgaaaa cagatatcta caggtaaact aagtgaaacg
     3901 acgcgtctta atcaaggaga actcatgacg tatcaacaag ctggacgcat tgctgttttg
     3961 aaaaggattt tgggttgggt gatttttata cctgcattga tctctacatt gatttctttg
     4021 ctgaaattta tgaatacccg gcaggaaaac caggaaggca ttaatgcggt catgctcgac
     4081 tttactcatg tcatgatcga tatgatgcag gcgaatacgc cttttttgaa tctgttctgg
     4141 tataactccc cgacgcccaa ttttaatggt ggcgtgaacg ttatgttctg ggtgattttc
     4201 atcctgattt ttgtcggact ggcgttgcag gattccggtg cccggatgag tcgccaggcg
     4261 cgcttcttgc gggaaggtgt tgaagatcaa ctcattctgg aaaaagccaa aggggaagag
     4321 gggctaacgc gtgaacaaat tgagtcccgt attgttgttc cgcaccatac tattttcctg
     4381 cagtttttct cactatatat cctgccggtt atttgtattg ctgcaggtta tgtgttcttt
     4441 tctctgcttg ggtttattta atcaaaaggc cgcacggagc ggccattatc ctcaacccat
     4501 atcatgctgc taataatcta tccaatgact gctgagcaat aaccaaatgc tgaccgccaa
     4561 ataatcttgc acgatttaac agcgtgtaga gttggtaaac cggttgacgt tcaaggaaat
     4621 cggcaggtag cggggatact gactgatagc cgtcatagat ttgtggcggc tgttcagtat
     4681 gcagcggtaa catcgccagg tcgcactctc ggtcacccca gtagcaggca gggtcgaaaa
     4741 tgtacgggcc atccggaccc agcgcacagt tgccggacca taaatcgccg tgcaacagag
     4801 aaggctgcgg ttgatgcgag gccagacgct gctgaatatg ctcgacgatg gtgtcgatat
     4861 tgccgaaagc gatccctttc tctgctgcca gttccaactg ccagccaatc cgttgttcag
     4921 caaaaaacgt tgaccagcga cgttgccagg tgttgggctg tggagttgtg gagagcgagt
     4981 tatcaaaatc gaggccaaat tgtggttggt cactccattg atgtaaacgc gcaatttgct
     5041 gaccaagaat aaatgcgcta tgcgcatcca gcggacgagg tgggagataa tccatcacca
     5101 gaaaactgta gtcacggtca gcgcctactg cccaaacctt aggcacggtg acggttttac
     5161 tacgcgacag taactccagt tggtcggctt cggcggtaaa accgggaagc agttcccttt
     5221 catcacattt gacgaaaaag tcatgtcctg catagcgcaa atgccatgcg gcatggactt
     5281 ctccgccagg cagttcatta cgcagttcga tttcgccttc acctaactgc tcgctcaaaa
     5341 gacgactgat tgcctgccac atgatgtctc tcccatgttg tctgccagat cataaggtta
     5401 gcgcaagaat gcggtgtaaa aacacgagct aacgcacacc acagactgat tattgcccaa
     5461 aaaccattta ttttctgttg ctattccatt cctcccaggt cgtgatggtg atttcaggat
     5521 ctttgccggt tgcactttgg gtaagccccg aaaccagttc tctaatttct tcttcacttt
     5581 gggtactgat aagtccaaaa gtgttagtcc ccagctcatg gatatttccc tcatcatccg
     5641 tcatggtgag caaaaaaccg tcgcgagtca ggtaattatt caactcgtta atttcagtca
     5701 atgtatcctc atgcaacatg acggttatga cataacggac aagatcgcca ctggccatag
     5761 ttcacccctt tgttatcaca tgacttttga gcatagtcgg agaaacgcgt tgccgacagg
     5821 ttggtggtga ttcccccaat gctgggggaa tgtttttgtt agcgggaaag gtaagcgtaa
     5881 attttttgcg tatcgtcatg ggagcacaga cgtgttccct gattgagtgt ggctgcactc
     5941 cccgcagcta cgccaaaacg gaccatctct tcaagagagg cattttctgc cagtttcagt
     6001 gtcatcgcgc cgaccatgct gtcaccagcg ccaacggtac tctggctttt caccggtggt
     6061 ggcaccacct gaatgcagtt ttcactatca acacccagcg ctccttgtgg accaagggaa
     6121 acgacaaccc gtttggcctt gccgctatta acgatttcct gcgcggcttt gcggacatcg
     6181 tctggctggg tgagttcgcg attcaccagc gcactgagtt ctttttggtt aggcttaacc
     6241 aactcgatgt taccaatcgc cagtgctgca cttaacgctt cgccagaact gtcgatgatg
     6301 cagcggatcc cttggttttg cgcagcggaa atcagttggg ttaatttttc cagcttcaca
     6361 cctggcggca ggcttccgct tatgaccagg atggccccgg attcaatttc cagaacttgc
     6421 tcttcaagct ggcgaaactc atcttcattt aatgccgcgc ctggcataac aaaacgatac
     6481 tgctcaccgc ttgcttccac atgtacgtgt aaattctgcc gggtccagtc tttggcttct
     6541 acagtagcga cggggacatt ttcatccgcc aacagagaaa ccaggtgttc gccggtcgcg
     6601 ccacccgccg ggaagatcgc tgtggcactg cctccaagat gggcaatggc gcgggcgacg
     6661 ttgatgccgc cgcccccggg ttcgaacacc ggtgcggtac agcgcagttt tccttcggga
     6721 taaatttgcg gggtaattgt tgcgctatcg agagagggcg caagtgtcaa cgtatagata
     6781 cgtaccatca tttcctccta taggctgatt tcagtttggc accgaatcag ccgaaagtga
     6841 agaaattaac aatatgattt attagagctt tatttaaaat tttgcagata aatatatata
     6901 aataaaaatc tgttttcgcc acatgcttat tcaaaaaatg aaataagaat acattgctaa
     6961 gttattcgcg cctttttata attcgttacc tttccaggga cgctaatctt tgatccttaa
     7021 gaaagtttcc gggaccgcga tggtctcttt ttttgttgta cggactcttg ataaatgaag
     7081 cttttgaaga cagttcctgc catcgttatg ttggcggggg gcatgtttgc gtcactgaat
     7141 gcagctgccg atgattccgt ttttactgtc atggacgatc ctgccagcgc taaaaaacct
     7201 ttcgaaggta atctgaacgc aggatatctt gcacaatcag gcaatacgaa aagctcctca
     7261 cttacggctg acaccaccat gacctggtat ggacaaacca ctgcctggtc gctgtggggg
     7321 aacgccagta atacttcttc taatgatgag cgttcttcag aaaaatatgc ggcgggcggg
     7381 cgtagccgtt ttaatttaac tgattatgac tatttatttg gtcaggcaag ctggctgaca
     7441 gaccgttata acggctatcg agagcgcgat gtgttgaccg cgggttatgg tcgtcagttt
     7501 ttgaatggcc cggtacacag tttccgcttt gaattcggtc caggtgtgag ttacgacaaa
     7561 tataccgaca atgccagtga aactcagccg ctgggctatg cctccggtgc ttatgcatgg
     7621 cagttgactg acaacgctaa atttacgcag ggtgtttccg tctttggcgc ggaagacaca
     7681 acgctgaact ccgaaagcgc attaaatgtt gctattaatg aacattttgg actgaaagtg
     7741 gcttacaacg tcacatggaa ctctgaacct ccagagtctg caccggaaca taccgatcgt
     7801 cggacaacct tatcgctggg ctacagcatg taaaattatc tgccggaccg tgttatgcgg
     7861 tctggctgtc ttcgtttgtc actgctcagc cccaaatcat tgctgcccaa cgtagcaaca
     7921 acatagcgcc acagattgcc agtagcacca ccaccatctt ccagtgtttt ttatcaaagc
     7981 gtttagtcgg cataccagtg tcctgcatat ctcttgtgcg tgataacgga gtgtaacaaa
     8041 agggacaggc atcaagggat tttttgttat tacagccgtc gggtggcgtt gtacacctga
     8101 tcactgtttc tgcgttttat gaaaaaaata aaa
//
LOCUS       DAZGVQ010000203         8119 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000203 DAZGVQ010000000
VERSION     DAZGVQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.205"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..292)
                     /locus_tag="ACHLAG_004649"
     CDS             complement(<1..292)
                     /locus_tag="ACHLAG_004649"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657754.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKI"
     gene            576..2207
                     /gene="oppA"
                     /locus_tag="ACHLAG_004650"
     CDS             576..2207
                     /gene="oppA"
                     /locus_tag="ACHLAG_004650"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFP0657755.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2293..3213
                     /gene="oppB"
                     /locus_tag="ACHLAG_004651"
     CDS             2293..3213
                     /gene="oppB"
                     /locus_tag="ACHLAG_004651"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFP0657756.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3228..4136
                     /gene="oppC"
                     /locus_tag="ACHLAG_004652"
     CDS             3228..4136
                     /gene="oppC"
                     /locus_tag="ACHLAG_004652"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFP0657757.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            4148..5161
                     /gene="oppD"
                     /locus_tag="ACHLAG_004653"
     CDS             4148..5161
                     /gene="oppD"
                     /locus_tag="ACHLAG_004653"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFP0657758.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            5158..6162
                     /gene="oppF"
                     /locus_tag="ACHLAG_004654"
     CDS             5158..6162
                     /gene="oppF"
                     /locus_tag="ACHLAG_004654"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFP0657759.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6215..6544)
                     /locus_tag="ACHLAG_004655"
     CDS             complement(6215..6544)
                     /locus_tag="ACHLAG_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFP0657760.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6579..8039)
                     /gene="cls"
                     /locus_tag="ACHLAG_004656"
     CDS             complement(6579..8039)
                     /gene="cls"
                     /locus_tag="ACHLAG_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP0657761.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
BASE COUNT         2067 a         1930 c         2054 g         2068 t
ORIGIN      
        1 ttattttcgc cacatcataa tcctgggctt gctgaagaat aattgaaatg atattagtaa
       61 ttccactgcc tttggtagag gaaagtgcta aataataatc aattgttaaa ttattgtgca
      121 tttcactact ggaactgtaa tcagaaaaga tagacatgct tagccaatct ctatttgatt
      181 gaattgaaag atgtttgtta aggcatggat gcaagctata gattctgata cggtcaataa
      241 aagagaattg cttaacaatt ttgcaaaatg tattggcgag taagaaccgc atttggtact
      301 ttccgggcaa ccgccagacg attctttatt ggtaatgaga ataattaaca attaaagagc
      361 gtcgcgaaag aataatgtgt ctcgacaggg gagacacagt acgaatcgac ataaggtgat
      421 cgtctgaatc accagaataa ataaagtcgg tgatagcaat ccgtaacgat aaagtaacct
      481 gacagcagaa agtctccgag cctgtgcagg gtcccaatcc gggattacac atgctggtta
      541 ataccagtaa ttataatgag ggagtccaaa aaacaatgac caacatcacc aagagaagtt
      601 tagtagcagc tggcgttctg gctgcgctaa tggcagggaa tgtcgcgctg gcagctgatg
      661 tacccgcagg cgtcacactg gcggaaaaac aaacgctggt acgtaacaat ggttcagaag
      721 ttcagtcatt agatccgcac aaaattgaag gtgttccgga gtctaatatc agccgagacc
      781 tgtttgaagg cttactggtc agcgatcttg acggtcatcc ggcacctggc gtcgctgaat
      841 cctgggataa taaagacgcg aaagtctgga ccttccattt gcgtaaagat gcgaaatggt
      901 ctgatggtac gccagtcaca gcacaagact ttgtgtatag ctggcaacgt tctgttgatc
      961 cgaacactgc ttctccgtat gccagttatc tgcaatatgg gcatatcgcc ggtattgatg
     1021 aaattcttga agggaaaaaa ccgattaccg atctcggcgt gaaagctatt gatgatcaca
     1081 cattagaagt caccttaagt gaacccgttc cgtacttcta taaattactt gttcacccat
     1141 caacttcacc ggtgccaaaa gccgctatcg agaaattcgg cgaaaaatgg acccagcctg
     1201 gtaatatcgt caccaacggt gcctatacct taaaagattg ggtcgtaaac gaacgaatcg
     1261 ttcttgaacg cagcccgacc tactggaaca acgcgaaaac cgttattaac caggtaacct
     1321 atttgcctat tgcttctgaa gttaccgatg tcaaccgcta ccgtagtggt gaaatcgaca
     1381 tgacttataa caacatgccg atcgaattgt tccagaagct gaaaaaagag atcccggacg
     1441 aagttcacgt tgatccatac ctgtgcactt actattacga aattaacaac cagaaaccgc
     1501 cattcaacga tgtgcgtgtg cgtaccgcac tgaaactagg tatggaccgc gatatcattg
     1561 ttaataaagt gaaagcgcag ggcgacatgc ccgcctatgg ttacactcca ccgtatactg
     1621 atggcgcaaa attgactcag ccagaatggt ttggctggag ccaggaaaaa cgtaacgaag
     1681 aagcgaaaaa actgctggct gaagcgggtt ataccgcaga caaaccgttg accatcaacc
     1741 tgctgtataa cacctccgat ctgcataaaa agctggcgat tgctgcctct tcattgtgga
     1801 agaaaaacat tggtgtaaac gtcaaactgg ttaaccagga gtggaaaacg ttcctcgaca
     1861 cccgtcacca gggcactttt gatgtggccc gtgcaggctg gtgtgcagac tacaacgaac
     1921 caacttcctt cctgaacacc atgctgtcga acagctcgat gaataccgcg cattataaga
     1981 gcccggcctt tgacagcatt atggcggaaa cgctgaaagt gactgacgag gcgcagcgca
     2041 cagctctgta cactaaagca gaacaacagc tggataagga ttcggccatt gttcctgttt
     2101 attactacgt gaatgcgcgt ctggtgaaac cgtgggttgg tggctatacc ggcaaagatc
     2161 cgctggataa tacctacacc cggaatatgt acattgtgaa gcactaatgg caatacgtgg
     2221 ggcaggagtg tcctgctcca cggtgtctga tttttatcgc attacagaag gcacaggcca
     2281 gaaggtaggg caatgttaaa atttattcta cgtcgctgtc tggaagcgat tccgacgcta
     2341 tttattctta ttactatttc gttctttatg atgcgcctcg cgccgggaag cccttttacc
     2401 ggcgaacgta ctttaccgcc ggaagtgatg gccaatatcg aagcgaaata tcatcttaat
     2461 gatccgatca tgacacagta tttcagttac ctgaaacaac tggcgcacgg tgatttcggt
     2521 ccatcgttta aatataaaga ttattcggtc aatgacctgg ttgcatccag ttttcccgtt
     2581 tctgccaaac tgggagccgc agcatttttc cttgcggtaa tactgggtgt tagtgctggc
     2641 gttattgccg cattaaaaca aaacaccaaa tgggactata ccgtgatggg gctggcaatg
     2701 accggggttg ttatccccag ttttgtggtt gcgccattat tagtcatgat atttgcgatc
     2761 attttgcatt ggctgccggg cggtggctgg aatggtgggg ctcttaaatt catgatattg
     2821 ccgatggtgg cgttgtcact cgcttatatc gccagtattg cgcgtattac ccgtggctct
     2881 atgattgaag tattacactc caattttatt cgtactgccc gggcgaaagg gttacctatg
     2941 cggcggatca ttttacgtca cgcattaaaa cctgctctgt tacccgtgct ctcttatatg
     3001 ggccccgcat ttgtcggcat tattaccggt tctatggtta tcgaaaccat ttatggtttg
     3061 ccggggattg gacaactgtt cgttaatggc gcattgaacc gtgactattc cttagtgtta
     3121 agcctgacca tcctggttgg tgctttaacc attttgttta atgccattgt cgatgtgcta
     3181 tatgcggtta tcgacccgaa aatccgttac tgatactgga gctcgcgatg atgttaagta
     3241 agaaaaacag cgagacgctg gaaaatttca gtgaaaagct ggaggtcgaa gggcgcagct
     3301 tgtggcagga cgcacgtcga cgttttatgc ataaccgtgc ggcggttgcc agtctgatag
     3361 tgctggtgct gatcgcgtta tttgtaatcc tggcaccgat gctttcccag tttgcctatg
     3421 acgatactga ctgggcgatg atgtccagcg ccccggatat ggagtccggt cactactttg
     3481 gtactgactc atccggtcgc gacctgcttg tgcgcgttgc gattggcggg cgtatctcac
     3541 tcatggtcgg tgttgctgcg gcactggtgg ctgtggtcgt cgggacactt tatggttcgc
     3601 tttccggtta tctgggcggt aaagtggatt cggtaatgat gcgtctgctg gaaatcctca
     3661 actccttccc attcatgttc ttcgtcattt tgctggtgac ctttttcggt caaaacatcc
     3721 tgctgatttt cgtggcgata ggcatggttt cctggctgga tatggctcgt attgtgcgtg
     3781 ggcaaaccct gagtctgaag cgcaaagagt ttattgaggc ggcacaagtt ggcggtgtat
     3841 cgacatcggg cattgttatt cgccacattg tgccgaatgt actcggtgtg gtggtggtct
     3901 acgcatcact tctggtgccc agcatgatcc tctttgaatc tttccttagc ttcctggggt
     3961 tgggtacgca agagccgtta agcagctggg gtgcattgct gagtgatggc gcgaactcga
     4021 tggaagtctc tccatggtta ttgttgttcc cagcgggatt cctcgtggtg acgctgtttt
     4081 gtttcaactt tatcggcgat ggcttgcgtg atgccctcga cccgaaagat cgttaaggag
     4141 tgcagccatg agcgtaattg aaactgcaac tgtgccgctc gcacaacaac aggctgacgc
     4201 actgctgaac gtgaaagatt tgcgtgtcac ctttagtacc ccggacggcg acgtcacggc
     4261 ggtcaatgat ttgaattttt ccctacgtgc cggagaaacg ctgggtattg taggtgagtc
     4321 tggttcgggt aaatcgcaaa ctgcatttgc gttgatgggc ctgttggctg ccaacggacg
     4381 tattggcgga tcggcaacct tcaatgggcg tgaaatcctc aatttgccag agcatgaact
     4441 caataaactg cgcgctgaac aaatctcaat gatttttcag gacccaatga cttcgttgaa
     4501 tccctatatg cgcgtcggtg agcagttaat ggaagtgctg atgctgcata agaacatgag
     4561 caaagctgag gcgtttgaag agtcggtgcg gatgctcgat gcggtaaaaa tgccggaagc
     4621 gcgtaaacgc atgaaaatgt acccgcacga attttctggc ggcatgcgtc agcgagtcat
     4681 gattgcgatg gcattgctat gtcgacctaa gctactgatt gcggatgaac ccactactgc
     4741 gctggacgtc accgtacagg cgcagatcat gacgctatta aatgaactga agcgggaatt
     4801 taataccgcc atcattatga tcacccacga tcttggcgtg gtggcgggga tctgtgacaa
     4861 agtgctggta atgtacgccg ggcgcacgat ggaatatggc aatgcgcgcg atgtctttta
     4921 tcaacccgtt catccttatt ctatcggttt gctcaacgcg gtgccgcgtc ttgatgcgga
     4981 aggtgaaaca atgttgacca tcccgggtaa tccgccaaac ttgctgcgat taccgaaagg
     5041 ttgcccgttc cagccacgtt gtccacatgc gatggaaatt tgtagtagcg caccgccgct
     5101 ggaagagttt acgcctggcc gtctgcgtgc ttgctttaaa ccggtggagg aactgttatg
     5161 aatgctgtaa ctgaaggaag aaaagtcctc cttgaaatcg ccgatcttaa agtgcacttt
     5221 gaaatcaaag atggcaaaca gtggttctgg caaccgccga aaacgctcaa agccgtcgat
     5281 ggtgtcactc ttcgcctgta tgaaggggaa acattaggtg tggtagggga atcgggatgc
     5341 ggtaagtcca cctttgctcg cgccatcatc ggtttggtca aggcgaccga cggtcatgtt
     5401 gcctggttag gtaaagagtt gctgggcatg aagcccgatg aatggcgtgc cgttcgcagt
     5461 gatattcaga tgattttcca ggatccgttg gcatcgctaa acccgcgtat gaccatcggc
     5521 gagatcatcg ctgaaccact gcgtacttat catccgaaaa tgtcacgcca ggaagttcgc
     5581 gagcgcgtga aggcgatgat gctgaaagtc gggttattgc ctaacctgat taaccgctat
     5641 ccgcatgagt tctccggtgg gcagtgccag cgtatcggga ttgctcgtgc tcttattctt
     5701 gaaccgaagc tgattatctg cgatgagccg gtttcggcgc tggacgtgtc aattcaggcg
     5761 caggtggtca acctgctcca gcagctgcaa cgtgagatgg gattgtcatt aatttttatc
     5821 gctcatgacc tggccgtggt aaaacacatt tccgatcgtg tgttggtgat gtatctcggc
     5881 catgcggtag aactggggac ctatgatgag gtctaccaca atccactaca tccttacacc
     5941 agggcattga tgtcggcagt ccccatacct gatccggatc tggagaagaa caaaaccatc
     6001 cagttactgg aaggggaatt accgtcgccg atcaacccgc cttccggttg tgttttccgt
     6061 acccgttgcc cgattgccgg tccggagtgc gccaaaacac gtcctgttct ggaggggagt
     6121 ttcagacacg ccgtttcttg cctgaaagtc gatccgcttt aaacacaata agggctgaca
     6181 gttgtcagcc ctttttcacg ctaaaagcga ttatttattc ccgccagatg atatggcaaa
     6241 gtttgtgatc tttttcgcgg cataacagaa tgcgggcgaa aacatcgttg atttcaccat
     6301 cttcactgtc cgccagacca atcaccactt cggcaaaaaa gtctgggttc aaatcgaaat
     6361 caacatgttc ctgccagtct tccgccggat cgaataactc cgcgccgccg cgctcttcaa
     6421 actgaagatt gaacagcaga acatcagctg gatcgagatt gtcggcagcc agttcgagaa
     6481 aaatatcgta ggcctgctca agcgtttcat cttcagtcag gcgattgttt agatccatat
     6541 ccatagtcac tacctgttta acctctgttg gcgacgtttt acagcaacgg actgaagaag
     6601 taaaacagtc gctcggcgac acgttgccat aatggacgtt ttagccataa acgggcatcg
     6661 agcagacgtg aacgcgaaat ataatcgtcc tgaacggcgg caaggtctgc accaaaacct
     6721 ttatcgtcga tcgccagggt aatctcgaaa ttcagccaca gactccgcat atcaaggtta
     6781 acggtgccaa ccagacttag ttcgccatcg accagcacgc tcttggtatg cagtaacccg
     6841 ccttcaaact gataaatttt aaccccagca gcaagaagtt ccgtaaagaa tgcgcgactg
     6901 gcccagccga ccagcatcga gtcatttttt cgcggaagga taatactgac atccaccccg
     6961 cgctgcgccg ccgtgcaaat cgcatgaagt aaatcatcgc ttggcacaaa gtagggcgtg
     7021 gtcatgatca aatattcacg cgccgaataa gccgcagtca ataatgcctg gtgaatgaga
     7081 tcttccggaa agccggggcc agaagcaatt gtgtgaatgg tgtgaccgct ggcctgttca
     7141 aacggcataa tattgacatc tggtggtggc ggcagaatac gttttccggt ttcaatctcc
     7201 cagtcgcagg aataaataat ccccatcgcg gtggcgatgg ggccttccat acgcgccatc
     7261 agatcaatcc attgccctac gcccgcatct tgtttgaagt agcgaggatc gaccatattc
     7321 atgctgccgg tgtacgcgat gtaattatcg atcatgatca tcttgcgatg ttggcgcagg
     7381 tccatacggc gtaaaaacac acgcatcaga ttgaccttta aggcttcgac cacttcaata
     7441 ccggcattac gcattagctc gggccacggg ctgcggaaaa aagccacact cccggcggag
     7501 tcgagcatca atcggcaatg aataccacgc cgtgcagccg ccattaatga ttcagccacc
     7561 tggtccgcca tgccgccggg ctgccagata taaaacacca tctcaatatt atggcgcgcg
     7621 agctggatgt cgcggattaa cgcctgcatc acatcatctg actcggtcat cagttgtagc
     7681 tgattccctt tgaccccagc gatcccctga cgacgctcgc aaagcttgaa taatggcgca
     7741 gcgacactgc tattttcttc ggcgaagata tgcttacagg ctttaaggtc gttaagccat
     7801 tttgcggtgg aaggccacat cgctctggcg cgctcagcgc ggcgtttgcc taaatggagc
     7861 tcgccaacgg caagataggc aataattccg actaacggca gaatgtaaat aatcaacagc
     7921 caggccatcg cggagggaac tgcgcgtcgt ttcattagaa tgcgtaaagt tacgcctgca
     7981 atgagcaacc agtatcccag aatggccaac caactcacca acgtataaac ggttgtcata
     8041 gattagaaat ccttttgaaa gcgcattgtt atgagtttac gcatatggat tcatctggca
     8101 aataaaaacg cgggaaaag
//
LOCUS       DAZGVQ010000204         7958 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000204 DAZGVQ010000000
VERSION     DAZGVQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.206"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(22..1032)
                     /gene="pyrD"
                     /locus_tag="ACHLAG_004657"
     CDS             complement(22..1032)
                     /gene="pyrD"
                     /locus_tag="ACHLAG_004657"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFP0657762.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(1143..1880)
                     /gene="ycbF"
                     /locus_tag="ACHLAG_004658"
     CDS             complement(1143..1880)
                     /gene="ycbF"
                     /locus_tag="ACHLAG_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFP0657763.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAIRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTWNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(1846..2361)
                     /gene="ycbV"
                     /locus_tag="ACHLAG_004659"
     CDS             complement(1846..2361)
                     /gene="ycbV"
                     /locus_tag="ACHLAG_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0657764.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(2369..2911)
                     /gene="ycbU"
                     /locus_tag="ACHLAG_004660"
     CDS             complement(2369..2911)
                     /gene="ycbU"
                     /locus_tag="ACHLAG_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0657765.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(2923..3993)
                     /gene="elfG"
                     /locus_tag="ACHLAG_004661"
     CDS             complement(2923..3993)
                     /gene="elfG"
                     /locus_tag="ACHLAG_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0657766.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(3984..6583)
                     /gene="elfC"
                     /locus_tag="ACHLAG_004662"
                     /pseudo
     CDS             complement(3984..6583)
                     /gene="elfC"
                     /locus_tag="ACHLAG_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            complement(6608..7309)
                     /gene="elfD"
                     /locus_tag="ACHLAG_004663"
     CDS             complement(6608..7309)
                     /gene="elfD"
                     /locus_tag="ACHLAG_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP0657767.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            complement(7392..7934)
                     /locus_tag="ACHLAG_004664"
     CDS             complement(7392..7934)
                     /locus_tag="ACHLAG_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0657768.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
BASE COUNT         2047 a         2014 c         1761 g         2136 t
ORIGIN      
        1 aagaggaatt cgaagaaata attagatatg ggtaacgatt tctttaatca gcggcggacc
       61 tttaaaaata aaaccagaat aaatttgcac cagtgaggca cccgcagcaa tcttttcacg
      121 cgcagcgata accgagtcga tgccgccaac accgatgatc ggtaagcgac cgtttaattc
      181 cagtgacaag cggcgaataa tttcggtgct ttttaactga agcggacgac cacttaagcc
      241 accggtttga tcgcaatttt tcattccctg aacaagagaa cgatcgagtg tggtattggt
      301 tgcaataacg ccatcaatat tatggcgaac taaactatcg gcaacctgga tcaattcttc
      361 ttcagaaaga tccggcgcga tcttcactgc gatcggcaca tatttatggt gcatcgcttg
      421 caaatcattt tgcttatttt taatcgcggt taagagatca tccagcgctt caccatattg
      481 cagtgtgcgt aatcctgggg tattcggcga tgaaatattg atggcgatat atcccgcata
      541 ggcatagatt ttttccatac aaatcagata gtcatctttg ccctgctcca ctggcgtatc
      601 tttatttttg ccgatgttaa tacccaggac gccgtcataa tgggcctttt ttacgttctc
      661 tacgaggtta tcaacgccaa gattattaaa gcccatacgg ttgatcaaac cttcggcatc
      721 taccagacga aagagacgcg gcttgtcatt acctggctgt ggacgtggcg tgacggtacc
      781 gatctcgatc gatccaaatc ccatcgcgcc taacgcgtca atgcactccc cgtctttatc
      841 aagaccggct gccagaccaa gcggattttt aaacgtcagg cccatgcagt taacaggttt
      901 cgcaggcact ttctgccgca ccagtgcttc aaacggcgtt cctgtaatac ggcgtaattg
      961 ctgaaaagta aactcatgag cgcgctctgg atcgagctgg aaaagggctt tacgaacgaa
     1021 ggggtagtac atgaactctc ctggattccc ggtgtgcaaa ccgggggcgt attatgtgcg
     1081 agttcggacc aaaagggaat tgacctgcgg caaaaaaaac gcaaacgttt tccgaacctg
     1141 tttcagggca gattttgctc cttctttgca ctcaacccgc cgtaatcatt tatcgcccgc
     1201 caggcgacat gacacggtgc gattgcctga caccagcttg tctgacgctg gcttttaggc
     1261 ggcacaactc ccgcattact taacgcttta ccatttacgc tcacctgaat caggttaatg
     1321 tagtaaggcg ttgggttagt gagttgtaca ccctgcgaat tccatgtcca gcgtaattgt
     1381 tgataagctt ccagcgggtc gcccggcaaa ccttcgggtc gccagaacag tttaaatacc
     1441 gagcgtatcg ccacttttac gctctgattt tcaactttgc cggatggcac gctggcaatg
     1501 cttaactcaa atagcgtttc gcgatccaca ggcaagatgt cgctttccgt tctcagcaag
     1561 cgcaacgtgc ctgtagtacc gggcttcagg agaataagtg gcggagcggc cagtaatggt
     1621 gctggcactg tcgacgcttc ccccccggcc caacgcgttg ggcggttgat tttgctgtta
     1681 ataagccagg attcctgact ggtattagtc agtaaaatag atatcgattc tctgtctgcc
     1741 ggaaagataa atcgcgttcc gccgaccacc actcctgcct gcaattcacc cgccaccagc
     1801 agcgataaaa cggcgaaaat caagagcgcc aatctgttcc acgtattagt cataatgaag
     1861 tacaaatgtc gcgctggcat tcactttacc cgactgcaca tcgctattga caggtcgcaa
     1921 ttgcgcataa aaagttaatg gcaccttacc gctatgtagg ctgtagtctt tactcgcctg
     1981 gttaagcgga atcaatatct tcgcatcatc cagaatcgca attgccagcc cgctggctgc
     2041 atccgtacca ctttcaatgg acagcatttc gctctgcact ccgtcggggg ttcctgaaaa
     2101 ggtgacgtca accgtgctga catcagttcc gcaatcctgc aaattaatga tgaaagattt
     2161 ctccgggcca taattcccat gaaaaaaacg tgtcagcgag acatcgccta actgcacatt
     2221 gatttgcttg ctatcctgtg ccagcacgca tgtattacgg cgaaacgatc cgctaatgtt
     2281 gactgtgcca tcatgggcaa atagctcaca gccccccgtt aatcccagta agaacagaat
     2341 ccagattatc cgtttcaaca tcactccttc actggtaaac cagatcaaaa tagagaaccg
     2401 ccgtcgcatt accgcccgtt gctcccgcct ttgtggactt ataacgaagt tgatatttga
     2461 gtgtgttatc cccggctttt aagggcgtaa gaggctggac ctgattgagc gggatttctt
     2521 gctgactttg cgcatcaaga atttgcacgg caatccccgt tgccatacca ccgcttccgc
     2581 ctgtgtcagt tagtttgagc agtgtcggcg caagggtgtc agcctcgccg ctaaaaagga
     2641 cgtccgcgcc agtaatacca gcggcacaac cggtgatatc gatattcaaa tcagcagcag
     2701 tgcttacggt attcgcggcc tgaaaatcac tgatattaaa atcaccaata tgaatatttt
     2761 gtaatgcact tttggtggcg acatcacagc cctgatcgac cacattccca taaatatgca
     2821 tactaacttg ttcaggccca gccatcccga catggatgtt aagaatgcta aagaacaaga
     2881 taacgcactg aaatatcgtt tttttcttca tacgttcacg cgttaatcaa aatttattct
     2941 caccgttgcc gtggcttcaa atcgcccggt ttctggtggt acacctgtcg tgctgactgg
     3001 atagacattc attcgcgcga tactgtctgc atcatcagga atagagagcg gtaatgttcc
     3061 accagcaggg gaaataatgt tgttctggct atcggtcacc accacgccca cgccaggacg
     3121 tgacgtctta atcgcctggg gatagctggg gtctgtggtt gccgtcagac tcaggttgac
     3181 gttaactaac cccgcgtttc ccgtgttaca cctgactgga attgcaagtt ctgccgttct
     3241 ggccccaaga ggcttatgcc ctacaacacg aaagttagcc gcaaatacgt cgcccagatc
     3301 gacctcgatt gtttctccgg cattgacgga acatgagcca agtgaattga tagtaccaga
     3361 caggtaatac actaaaaccg gtgtacctgt agtcgtgcag gaatcacctg cgggtatgtt
     3421 gtagcattca aataatcgcg ccagcgccat aggaggaatg acgaccgaac caacgaacgg
     3481 ctgcaatatt ttcagggaaa agctaccgct attaccgcta gccccctggc cacctaaacg
     3541 ttgaccgccg ggatcgcaaa tatgccatcg tgtatctgcc cctgtgccca cgttttcgaa
     3601 aggaaccgca gtaggagaac tgcctttaac ctgcaataaa actttggcgg caagatagtc
     3661 attaattttg taccagttgt cgcctaaata gactaacggt gaatccgcgg cgaatgccca
     3721 tcggccactt ctgacattgt ctttatcgca atcacaggaa acataataat cattcgattc
     3781 gttccactga taaaatttgt ctttaactac tccagccgta ttttcttctt cctccagggt
     3841 tttaacgcca aaattgacgc caatatacgg attcccctga gtggtatgac actgcccgct
     3901 ataagcagca gtttgagcgc accatagcat taagacggcg gcaaaaaata gtcgtagtaa
     3961 taacacgcat ttttctccaa agattatctg catatggcat ccattcgcgt aatcgcagag
     4021 ttaatttgtt gttcggtaag ctggtaactg atatgacaaa tttgatcggg agcttcgccc
     4081 cagcgtacag ttatcgctcc ctgagcgggc aaaccggaca tatagagcga gccatcatca
     4141 tcaataatgc cgctattagt ctgtatgccg tccagcgtcg ctatcgcgcc aaaacgcagc
     4201 ggtataccct gctttgatgt tttcattaat acccttgctc ccgcatgggc attgaactcc
     4261 gccagaacca acgccccttt ggtgggaacc acgttgacta ctgcattttc aagatccacg
     4321 ttacgtttta acgatgccgc atcaagtgcg attcgatttt cccgataaga agtggcatag
     4381 gggacaacgg tatatccgcg ccagtcagtt ttcagcccgg tgctgttcgc cacacgagta
     4441 ttctctgcgc ctggtgctgc aatcaggacg ttagtttccc ccagcgattg ccccagggta
     4501 atgcccagtg aatgggcaac taaactgcct gaaagagcgt agttcagttg ttgttgactg
     4561 ccgttatcgc tgtagttgta gcccactcgg gcatctgcaa acgccccttt gtagtccatg
     4621 ctggcgctac cattagccgt tttcccctcg ctgttatatc cctgttgcac gctgtagctc
     4681 aggttaccgt catcgagtag tgtctccgtt aaacccaggt tttgtgtaat gtgcccttcg
     4741 ttatcgatac tggtgttaga ggtcatccag gcgtaatttt tttcctggta tagctatcat
     4801 tacttctggg gagcaacaga ttgagcggca gggaaaaatt tagtgcaaac acctgatcag
     4861 catcaggttg accacgggac ttactgtagt tccaggaaac ccccagcgag agatctttaa
     4921 tttgcgtgtt gtagccaaac tgtaatagcc gatcctgttg atcggtatgc cagtaagttt
     4981 gctggctacc acttaaataa aacgaaccgt actcgcctaa ttgctgggag atattgacct
     5041 gcagtttgcc acgtttggtg taaaacaaat tgtaataacg cgaccacatc ggcgtatctt
     5101 catcatcacc gtcattaaat tcgtagccat ccatatgttt atacatggtg tcggaaaggg
     5161 tgtagaaacc cgacgtcgaa tagcgatagc ccatcaattg aaaattagtg cccaattggt
     5221 tgagcgtttt ggcatacaga aaacgatatg actgaccttt atgttcgctt tggtctgcca
     5281 gcgtactctt cgcctgggtc acatcgaacg aaattgctcc gaaatcgcca aggttaaatc
     5341 ccagaccaaa catggcggca cggtaatatt cagcatattg tccaccacca taccatgtcg
     5401 ttccccacgg tccgccccac tgcaacgtgg cctgggcaaa tttgctctcc tgctgttcat
     5461 tactattggt tctgtatttc gccagtgtca ccgcgtattt gattcgcccc tgacgctgga
     5521 gtaatggcac gctggaaaac ggtacgctgt agctattgac gctgccgtcc gcttctttga
     5581 tttcaactaa caaatcaccg ctcgacgacg tggaatagag atcactaatt tcaaaagcac
     5641 caggcgatac ataagtttgg taaatggtgt aaccattttg cttaatcgtt atctgggcat
     5701 tgcttttggc gataccacgc accacaggcg caaacccttt caggctgtct ggcagcatgt
     5761 tgtcatctga cgccagctgc acaccacgaa agctgacact atcgaaaaaa tcgcctgcag
     5821 tataatcatc acctacggtc agttcgcctt ttagtggaat aaccgcccgc tgcaaatagc
     5881 tgctgagatt cttccattct gcggtttggc cactactgcg actccatatt gaattgttgc
     5941 gcaatctcca tgggcctaaa ttaacgccac tgtttaaatt cagaaaatag ctgtcgccag
     6001 aatcactgtc tgcgctgcta tgaatactgt tagccccgct aaacgaatat cccagtaata
     6061 gcgcgttgat cccttcatcc caacgttcag gggggacata accacgggca ttacttttga
     6121 tggcgatttg cggtactgaa atttccagac ggagtttatt cacattaaac gttacgctgg
     6181 catccggaat aatctccgcc aatggcacac acgcttgttt gtccagttgt ttaaaagccg
     6241 gaaacgcatc agtattaaca cccattgcat ccaggctttc agtcgtcaaa caggcggcaa
     6301 tccccttctc tggcgttatc tcaacaaaat tgacgttgcg cgtatctaca attgtctgat
     6361 tcagaacaat atcgacacga taaacccctg gcgttatttt ttgtcctttt tcgaaacgag
     6421 acaaatcggc caccattgag ggatcatctt tgaaaaaagc aggatcgaac caggtttcag
     6481 cttgtgcatt gaacgctgcc gacacaaaca ccaccagccc accaataaac gatggcactc
     6541 caccagagct taacaggctg tgttgtcgat gagttctata catctcgctc tcttaactga
     6601 agacggctta cgatttacag ttgagttgcg gcgtttccgc cccgtagtca ttgatggttc
     6661 gatacgttac gctgctattt ttggccccct ttggcagaga aaattggtat ttatcaaagg
     6721 gggaaatcat ttgattttta actacttttc cacctatttt caggtctgtc agggtaatga
     6781 aataaggtgt tgggttactg accgtaacca tgtcagctga cgaagtgcaa cgcagtttcg
     6841 ccggagcctc gcctaaggga tacgctaaac cttttggtcg ccagaaaagt ttgatgacgc
     6901 tttgtgtggc aatcttcagg gtatttccct cttcccgctt cgttgttgaa gggactgccc
     6961 gtacgctagt gaagaaaagg gtttctctgt ctttcgccaa cggcgcacca atgtacataa
     7021 tgcgtaacag attctcatta gccggattca gcacaaatag cggtggtgta ataataaaat
     7081 ctgctgattt tttattatct ggggtcatca cccatgactg aataagataa ggaacatccg
     7141 gactggtgtt acgtacaccc agtgaaattt gctcttcacc ttctgaataa acaagacgtg
     7201 ttgctgcaag tccaatcccg cctttacctg ccgcccatgc ggatgagcag ctgagtaata
     7261 ttgccgttaa acttacggtc acaattccct ttgttatgca agttttcata aatagtcact
     7321 cttaaaaaat atgaatatcc cctccagaac actagagggg tactaattaa taaaacaatt
     7381 aatgagttct attaataata gttaatatgg aaatttgtta ccgcattcac attaccagaa
     7441 gtcgctttcc ccgtagcgat ataatcaact ttaaaggtca ctgtttgctc agcagaatcc
     7501 gctaactgaa tattcgacgt ttcggtaccg ggagttaccg atgtagaacc atctggcaga
     7561 tacatttgta gagcaacatt cgtcgctgca tcggtggtag cctggttggc aaatgctgta
     7621 ggttgcacgc cgtcagccgt accactaaag gtaaacgtcg catttttagt tacagtaaca
     7681 tcacagcctt ctaaggcaat tggtacatca gttttaacgc cagagactgc tccattagtt
     7741 tgcaacttgg tggcgctgac atttggcaat gtgactactg aatctttcgt cgcagctacc
     7801 agggtacaag ctggagcaat aactttacca ttaaatgtta ctttgccatc cgtgatagca
     7861 ttatcatcag ctgccataac gctggacgtt gcacatacca cagttataaa cgccgtcaat
     7921 acactttttt tcatcgtcat catcctgatc tcttcgta
//
LOCUS       DAZGVQ010000205         7944 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000205 DAZGVQ010000000
VERSION     DAZGVQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.207"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..4503
                     /locus_tag="ACHLAG_004665"
     CDS             <1..4503
                     /locus_tag="ACHLAG_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
                     /protein_id="HFP0657769.1"
                     /translation="NNGLINNKEYSLVGVEDGSHGVVNVTDKGHWSFLGTGEAFRYIY
                     IGDAGDGELNVSREGKVDSGIITAGMKETGTGNITVKDKNSVITNLGTNLGYDGHGEM
                     NISNEGLVVSNGGSSLGYGENGVGNVSITTGGMWEVNKNVYTTIGVAGVGNLNISDGG
                     KFVSQNITFLGDKASGIGTLNLMDGTSSFDTVGINVGNFGSGIVNVSNGATLNSTGYG
                     FIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGTGELNITTGGIVKARDTQIA
                     LNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLNVEGGEVYLGVFEPAVG
                     TLNIGAAHGEAAADAGYITNATKVEFGSGEGVFVFNHTNNSDAGYQVDMLITGDDKDG
                     KVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMGSSEVTIASPGTLDILA
                     STNSAGDYTLTNALKGDGLMRVQLSSSDKMFGFTHATGTEFAGVAQLKDSTFTLERDN
                     TAALTHAMLQSDSENTTSVKVGEQSIGGLAMNGGTLIFDTDIPAATLAEGYISVDTLV
                     VGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLNLLEHDDSHVGVQLVKA
                     QTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGFRLTTAPGDGLYVNYGL
                     KALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVRQVSLSNGQNDYQGATY
                     VQMGTLRTDADGALGNTRELNISNAAIVDLNGSTQTVETFTGQMGSTVLFKEGALTVN
                     KGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSISPNAEVSLDNTQGLGRGNIAN
                     DGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVGQFNIDTGSALSVNEQK
                     NLGDASVINNGLLTISTERSWAMTHSISGSGDVTKLGTGILTLNNDSAAYQGTTDIVG
                     GEIAFGSDSAINMASQHINIHNSGVMSGNVTTAGDVNVMPGGTLRVAKTTIGGNLENG
                     GTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPTDKMNVKGDTQGNTRVR
                     VDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVYTLAKGKGNDEKNWYLT
                     SKWDGVTPPDTPDPINNPPVVDPEGPSVYRPEAGSYISNIAAANSLFSHRLHDRLGEP
                     QYIDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVLQLGGDLAQWSSNAQDR
                     WHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQNDANKTGAYVDSWALY
                     NWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEGTLNTWYVQPQAQITWM
                     GVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDGKQREFQPYIEANWINN
                     SKVYAVKMNGQTVGREGARNLGEVRTGVEAKVNNNLSLWGNVGVQLGDKGYSDTQGML
                     GVKYSW"
     gene            4712..4978
                     /gene="hipB"
                     /locus_tag="ACHLAG_004666"
     CDS             4712..4978
                     /gene="hipB"
                     /locus_tag="ACHLAG_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFP0657770.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            4978..6300
                     /gene="hipA"
                     /locus_tag="ACHLAG_004667"
     CDS             4978..6300
                     /gene="hipA"
                     /locus_tag="ACHLAG_004667"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFP0657771.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            6607..6815
                     /locus_tag="ACHLAG_004668"
                     /pseudo
     CDS             6607..6815
                     /locus_tag="ACHLAG_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7248..7811
                     /gene="fimA"
                     /locus_tag="ACHLAG_004669"
     CDS             7248..7811
                     /gene="fimA"
                     /locus_tag="ACHLAG_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP0657772.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
BASE COUNT         2169 a         1702 c         2141 g         1932 t
ORIGIN      
        1 aataatggac taattaataa caaagaatat tcattggtgg gcgttgaaga cggttcccac
       61 ggtgtcgtca acgtgaccga taaagggcat tggagtttcc tcggaacagg cgaagctttc
      121 cgctatatct atatcggtga tgctggtgac ggtgaactta atgtctcgcg tgaaggtaaa
      181 gtagattcgg gaattatcac tgcggggatg aaagaaacag gcacaggcaa cattactgtt
      241 aaggataaga actccgttat cactaatctc ggaactaatc ttggttatga cggccacggc
      301 gaaatgaata tcagtaatga ggggcttgtt gtcagcaacg gaggaagctc actcggttat
      361 ggagaaaacg gcgtcgggaa tgtcagcatc accacggggg ggatgtggga ggtcaataag
      421 aatgtctata ccaccattgg tgttgcgggc gtcggaaacc tcaatattag cgatggcggt
      481 aagtttgtat cgcaaaatat tacttttttg ggcgataaag caagcggtat cggcacactg
      541 aacctgatgg atgggacatc atcgttcgat actgtgggta tcaatgtcgg taattttggt
      601 agcggtatcg taaatgtcag taatggtgcc acccttaatt caacgggcta tggatttatc
      661 ggaggaaatg cctccggtaa agggatagtt aatatttcaa cggatagtct ctggaattta
      721 aagacatcat ctactaacgc ccaattgcta caggtcggtg tattaggcac gggtgaactg
      781 aatattacca ccggaggtat agttaaagcg cgtgatacac agatagctct caatgacaaa
      841 agtaagggcg acgtgagggt ggatgggcag aactctcttc ttgaaacatt caatatgtac
      901 gtagggacat ctggtacggg tacgttaacc ctgacgaata gcggcacgct gaatgtcgaa
      961 ggtggagaag tttacttagg tgtttttgaa cctgctgtag gaacgctaaa cattggtgca
     1021 gctcacggtg aggcggcggc agatgccggg tatataacca acgcgacgaa agtagagttt
     1081 ggttctggcg aaggtgtttt tgtctttaat catactaata acagtgatgc cggctaccag
     1141 gtcgatatgc tgattacggg tgacgataaa gacggaaaag tgatccatga tgcaggccat
     1201 acggtgttca atgcagggaa tacttatagc ggtaaaacgc tggtcaatga cggcctcctg
     1261 accattgcgt ctcatacggc agatggggta acgggcatgg gttcgagtga agtcaccatt
     1321 gcaagccccg gtacgctcga cattctcgca tcaacgaaca gtgcaggaga ttacacgctg
     1381 accaatgcgc tcaaaggcga tggtttgatg cgagtgcagc tgtcatcctc cgacaagatg
     1441 tttggcttta cccatgcaac agggactgaa ttcgccggtg ttgcccaact gaaagacagt
     1501 accttcactc tggaacgcga caacaccgct gcgcttactc acgcgatgtt gcagtctgac
     1561 agtgaaaata ccacatcggt aaaagtggga gagcaatcca ttggtggact ggccatgaat
     1621 ggcggtacgc tcattttcga tacggatatt cctgctgcga cgcttgcaga gggatatatc
     1681 agcgtcgata cgctggttgt cggcgcgggt gactacacct ggaaaggccg taactatcag
     1741 gtaaacggga cgggcgacgt gcttatcgac gtgcctaaac cgtggaatga tcccatggcg
     1801 aataaccctc tgacgacgct caatttgctg gaacacgacg atagccatgt cggcgttcaa
     1861 ctggtgaagg cgcaaacggt tattgggtcg ggtggctcat taacgttacg tgatttacag
     1921 ggcgacgagg tggaagcgga caaaacgtta cacattgcgc aaaacggaac ggtggtcgcc
     1981 gagggtgatt atggattccg cctcacgacc gcaccaggtg atggtttgta cgttaactat
     2041 gggctgaaag cgctgaacat ccatggtggg caaaagctga cgttagccga acatggcgga
     2101 gcctatggcg caacggccga tatgtcggca aaaatcggtg gtgaagggga tctggcaatc
     2161 aatacggtgc gacaggtttc gctttccaac ggtcagaacg actatcaggg ggcaacctac
     2221 gttcagatgg ggacattacg taccgatgcg gatggtgcac ttggcaacac ccgggaactg
     2281 aacatcagca acgcggccat cgtcgatctt aatggatcta cgcagacggt agagacattc
     2341 accgggcaga tgggttcgac tgttttgttc aaagaggggg cgctgacggt aaataaaggt
     2401 gggatcagtc agggtgaact gacaggtggc ggaaacctga atgttacagg gggaacgctg
     2461 gctatcgagg ggcttaatgc acgctacaat gcgttaacca gcattagccc aaatgcggaa
     2521 gtcagcctcg ataatactca ggggttaggc agaggaaata ttgccaatga cggtctgtta
     2581 acgctaaaaa acgtgactgg cgaactgcgt aatagcataa gcgggaaggg tatcgtgagc
     2641 gcaaccgcca ggacagatgt agagctggat ggcgataata gccgctttgt ggggcaattc
     2701 aacattgata caggcagcgc gctcagcgtc aacgagcaga aaaacctggg tgatgcttcc
     2761 gttatcaata atggcctgct caccatctcc actgagcgta gctgggcgat gacgcacagt
     2821 atcagcggta gcggtgatgt gacaaaactg ggtaccggga tcctgactct taacaacgat
     2881 tccgcggcgt atcagggtac gacggatatc gtgggggggg aaattgcttt cggttccgac
     2941 tctgccatta atatggcaag tcaacacatt aatatccata acagcggtgt gatgtcggga
     3001 aatgtcacca ctgcaggtga tgtgaacgtt atgcctgggg ggacactgcg tgtcgctaaa
     3061 accacaatcg gcggcaacct ggagaatggc ggcacggttc aaatgaatag cgaagggggg
     3121 aaaccgggga atgtactgac cgttaacggc aactataccg gaaacaatgg cctgatgacg
     3181 ttcaacgcga cgctgggcgg cgataattcg cccaccgata aaatgaacgt gaaaggcgat
     3241 acccaaggga acactcgcgt tcgggttgat aacattggcg gcgtcggtgc acaaacggtc
     3301 aacggtattg aactcattga ggttggcggt aattctgcag gtaacttcgc gctgaccacc
     3361 ggaactgtcg aagctggggc ttacgtctac acgctggcta aagggaaggg gaatgacgag
     3421 aaaaactggt atctgaccag taaatgggac ggcgtaacgc caccggatac gcccgacccc
     3481 atcaataatc cccctgttgt ggatccggaa ggcccatcag tttatcgccc ggaggccgga
     3541 agctatatca gcaacattgc cgcagccaac tcgctgttta gccatcgttt acacgaccgt
     3601 ctgggtgagc cgcagtatat agattcactg cattctcagg ggtcggcaag cagtatgtgg
     3661 atgcgtcatg tcggagggca cgaacgttca agggccggtg acggtcagct aaatactcag
     3721 gctaaccgct atgtattgca gctaggcggc gatttggcgc agtggagtag caacgcgcag
     3781 gatcgctggc atcttggcgt gatggcaggt tacgctaatc agcacagtaa tactcagagt
     3841 aatcgtgtgg gttataaatc ggatgggcgc atcagcggtt acagcgctgg gctgtacgcg
     3901 acctggtatc agaacgatgc gaataagacc ggcgcttatg ttgacagctg ggcgctgtat
     3961 aactggtttg ataacagcgt cagttccgat aaccgttctg ctgacgacta tgattctcgc
     4021 ggtgtgacgg cctctgttga gggtgggtat acctttgaag cgggaacatt tagcggcagc
     4081 gaagggacgc tgaatacctg gtacgtccag ccacaggcgc aaatcacctg gatgggtgtg
     4141 aaagattccg accatacccg gaaagacgga acgcgcattg aaacggaagg cgacggaaat
     4201 gtgcaaacgc gacttggggt gaaaacctac ctgaacagcc atcaccagcg tgacgatggt
     4261 aaacagcgtg agttccagcc ttacattgaa gcgaattgga tcaacaacag caaagtctac
     4321 gccgtgaaga tgaatggtca aaccgtaggc cgtgaaggtg cgcgtaatct cggtgaagta
     4381 cgtaccgggg ttgaggcgaa agtaaataac aaccttagcc tgtgggggaa tgtcggtgtg
     4441 caactaggtg ataaaggcta tagcgatact cagggcatgc tgggagtgaa atatagctgg
     4501 taaaccgtat aagccgcatg tcgagatggc atgcggctta atattgccga cttcaaaggc
     4561 tttaaagcct cccttttatc cgcgatcgcg gatatcgcag cgtttatccc gtacagcgga
     4621 taagatgtgt ttccagattg actttatcct cactaaagga taaaactgat aatatcccct
     4681 taagcggata aatttgctgt ggacgtatga catgatgagc tttcagaaga tctatagccc
     4741 aacgcaatta gcgaatgcaa tgaaactggt tcgccagcaa aatggctgga cgcagagcga
     4801 gctggcgaaa aaaattggca ttaagcaggc gacaatttcc aatttcgaaa acaaccctga
     4861 caataccacg ctcacgacat tttttaagat tttacagtcg cttgaactct caatgacact
     4921 atgcgacacg aaaaatgcct cgccagaatc aacagaacag caagatctgg agtggtaatg
     4981 cctaaacttg tcacttggat gaacaaccag cgggtaggcg agttaacgaa gttagccaac
     5041 ggcgcgcaca cctttaagta tgcaccggag tggttagcca gccgttatgc cagaccgttg
     5101 tcactttcgc tgccattgca gagggggaat atcacctcag atgccgtatt taacttcttc
     5161 gataacctgt tacccgatag cccgattgta cgtgaccgga tcgttaaacg ttatcatgct
     5221 aaatccagac aaccgtttga tttattgtca gaaatagggc gagacagcgt tggtgccgta
     5281 acgttattac ccgaaaatga aaccataacg cgcccgataa tggcatggga aaagcttact
     5341 gaagccaggc ttgaagaagt attaacggct tataaagcag atatccctct gggcatgatt
     5401 agagaagaaa atgactttcg catctcggtt gctggcgcgc aggagaagac agcgctgctt
     5461 agaataggca atgactggtg cattccgaaa ggaataacgc cgacgacgca catcattaaa
     5521 ttaccgattg gcgaaatcag gcagcccaat gcgacgctcg atctcagcca aagcgttgat
     5581 aatgagtatt actgtctgct gctggcgaaa gaacttgggt tgaatgttcc ggacgcagaa
     5641 atcattaaag cgggaaatgt gcgcgcgtta gcggtcgaac gttttgacag gcgttggaat
     5701 gctgagcgaa cggttttact tcgcttgcca caggaggata tgtgtcagac attcggttta
     5761 ccttcatcgg tgaaatatga atcagatgga ggcccaggca tcgcgcggat catggctttt
     5821 ttgatggggt ccagcgaggc gctgaaagat cgctatgatt ttatgaaatt ccaggtcttc
     5881 cagtggttga ttggcgcaac ggacggtcat gcaaaaaact tctccgtatt tattcaggct
     5941 ggcggcagtt atcgactcac gccattttac gacatcattt cagcatttcc ggtccttggc
     6001 ggtacgggaa tacacatcag cgatctcaaa ctggcaatgg ggcttaacgc atccaaaggc
     6061 aaaaaaacgg caatcgataa aatttatccg cgacattttt tggcgacagc aaaggtgctg
     6121 agattcccgg aagtgcagat gcatgaaatc ctaagtgact ttgccagaat gattccggca
     6181 gcactggata acgtgaagac ttcattaccg acagattttc cggagaacgt ggtgacggca
     6241 gttgaaacca atgtgttgag gttgcatgga cggttaagcc gagaatacgg tagtaagtga
     6301 gatatggggt ttggtcgttg ttaatgaaca atcatgaccg ccaacgactg gattcttcct
     6361 tggtttatac tggtgcgcat aatgtatatt atgttaaatc atgtctgagt gatgcgaagt
     6421 gcttttctgg tagtcgttat tcgttcaggg ttcaagaact taagcctctc ctctttcata
     6481 attggcctgt gcatggagaa atagcctggt tattccctgc cccatgctaa gagcgcagtg
     6541 tctgtcgcat agacactgca tacgaaaaca ccagagggtg ctgttactta tttaaggacg
     6601 gatcaagcgg gaatgagtcc actccggtat cagaaatggc tacgtctcaa tgaagtcagg
     6661 cgaccgatgc tgaatgaaca ttacgatgtc accacagcag cctatgctgt cggttacgaa
     6721 agctatccca tttcagtcgg gaatattcgc ggatgtttgg agagtcaccc aagagagata
     6781 ttaccaggtt aagaaagtct gtcggtcagc tttaataaac cctgcactta tctgtttaga
     6841 gcaattcggt gttagtttca gcaagcaaac attaaccata gctaatgatt tatagccata
     6901 ttaaccattg gtagcccttg acctttcctg acacttccct tgttcgcaat aatattctgt
     6961 ttagtcacgg tttataaata acatgcataa tgaaatcgtc atgttattta gcgacataaa
     7021 atgtttttgc aaatactctc ttcaacaagt gcagcaataa acttgttgtg attaggtgtc
     7081 cttgtgattt aaagatatta tcgttcttcg tgaaatgtaa taaccctgga aactatattc
     7141 tcagggcaac aaccaatcaa gaatgattct tgtttttaag caataacaga atgacttaat
     7201 tgttaaccct ctttattcag ctacttaaat ataaattttg gagaattatg aaactcaaac
     7261 atgttggtat gattgtcgtt tctgtgttgg cgatgtcgtc tgctgcggta agcgcagccg
     7321 agggtgatga atcagtaacg accactgtta atggcggtgt tattcatttt aaaggtgaag
     7381 tggtaaatgc cgcttgtgca attgattcag aatcaatgaa ccaaacggtt gagctgggtc
     7441 aggttcgttc ttctcgcctg gctaaagcgg gtgacctcag ctccgccgtt ggcttcaata
     7501 tcaagctgaa tgattgtgat accaatgttt ccagtaatgc agctgttgca ttcctgggta
     7561 ctactgtcac cagtaatgac gatacgttag cgctgcagag ttcagcggca ggctctgccc
     7621 aaaatgtcgg tattcaaatt ttggaccgta cgggtgaggt attaatactt gatggggcca
     7681 cttttagtgc taaaaccgac ttgattgatg gcacgaatat actgccattc caggctcgtt
     7741 atattgctct cgggcagtcc gtagctggta ctgcaaacgc tgatgcgacc ttcaaagttc
     7801 aatatctata attcaatctt aaggtggcta tacgtcatct gaaattgaaa cagggtgtgt
     7861 gtggcatggc tacgcacacc cgagcaattt accaatattc atctcatttt taatgagaaa
     7921 tggtttgtat ttggagcgct atga
//
LOCUS       DAZGVQ010000206         7913 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000206 DAZGVQ010000000
VERSION     DAZGVQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.208"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..117
                     /locus_tag="ACHLAG_004670"
     CDS             <1..117
                     /locus_tag="ACHLAG_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001440016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFP0657773.1"
                     /translation="IAVIFNWPPAEIFAMTPAEVVAWRERAAARSGSHNHDE"
     gene            107..2899
                     /locus_tag="ACHLAG_004671"
     CDS             107..2899
                     /locus_tag="ACHLAG_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0657774.1"
                     /translation="MMNNLHIRVALAAADTLTQPLRRARETVGALSDSLRETQDSIRS
                     LERQSEAFAAAQTKFDEATRKIAAVQQQLNRLQQAEREGTVLSERQREMMASLTTRLE
                     RLNRVRDVHQRRLEDSRRELERHGIAAEGSSQTIQAAIRRTAEYNEELARQRQALAQV
                     TRAQQRYEQAQERLGRIRNAGAAALAAGAAGLYAGGRAVAPVMDLQRHGAVIAAQAGE
                     GASDAVGYTGVINHMVHTGITPDAQLAAEAVGAVRSTLGGLGAVSENELTRITRKMLD
                     MQAVMGGDIPQHIQAAAIMIKNGLARSSDEAVDLMVAGMQRMGPVMRDELPEILHEYS
                     THFRNMGLTGSETMTLLADMAQQGKFALDKTGDAIKEFSIRGSDMSKASAEAYALIGL
                     DAARMSSAVASGGTAARAALQKTASGLLQIVDPAERANAAIALFGTPVEDLSVDQIPA
                     FLGALARVSDRMADVSGVADRMGQTLRDNLSGDVARLTGTFSALRSGIIGEITDDLRG
                     LVRMATAWGERLQAWVNAHPELVRMIALTTGAVLALTTAMGALSVAAWMVVAPLLKLR
                     LGLALLRAIPAGLSVAGTLFGGLTGVISAVGAALGALTWPVVVAVGALVGGALLIWRY
                     WDRVRAFVSGFIEGVQSALAPFMATASRFAPLLDLVSGALSRILGWFKSLLTPVSSTR
                     DLLEQCTSAGQTFGSTLAGALATVFSPLKALIDGLAWVLEKLGVLPGEAERVKRKLDE
                     ARKTPVAWEWDPEQKKMVQKAWHWSPPGGASVPSGASGTQPPPVALPAGIAPDGNRNV
                     LRHLKGIEGNTGGLLAENRKRIGPGDIVFKHLPRALAVRGQWHEPVVAPSSGGMRALP
                     QEEIQVQQWPTARPAPRMHQQETFASAFQGEIHVHLHGVSSHNPRELARMVGDAVRAE
                     MNRMARAGTGSFRDRD"
     gene            2910..3401
                     /locus_tag="ACHLAG_004672"
     CDS             2910..3401
                     /locus_tag="ACHLAG_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0657775.1"
                     /translation="MMMIYGMFVFQLSTLPHQQVEQSKRWRHVSNERVNRSSSWQYIG
                     SGEDHITMSGVLCPEITGGEVSLTALNTLAYTGLPWPLIDGVGQIYGMYVITEIRTTR
                     QELDRYGMARKIEFTVSFQRCNEDLRERLQSGSVGDLMAGLKNSASSALSSASSALSG
                     LML"
     gene            complement(3412..3999)
                     /locus_tag="ACHLAG_004673"
     CDS             complement(3412..3999)
                     /locus_tag="ACHLAG_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFP0657776.1"
                     /translation="MHTIKSARNAAYNRDGTIDCEIQFEGEESWLPYTATEYDNSPHG
                     KKLWGELKAGNHGPVSEYQENDTDRENARRLKNDEINAWRNAMEAASYTFEHNGRKWD
                     YGKSTQARLEPSVAAAKAGILPLDFFWTDAQNNDVPVSADELIALSDAAAKALFAKGM
                     EIHIRQRNMKKEIAALNDTAAILDYKVGWGKEAEA"
     gene            complement(4011..4442)
                     /locus_tag="ACHLAG_004674"
                     /pseudo
     CDS             complement(4011..4442)
                     /locus_tag="ACHLAG_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764700.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(4749..5351)
                     /locus_tag="ACHLAG_004675"
                     /pseudo
     CDS             complement(4749..5351)
                     /locus_tag="ACHLAG_004675"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(5351..5974)
                     /locus_tag="ACHLAG_004676"
     CDS             complement(5351..5974)
                     /locus_tag="ACHLAG_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFP0657777.1"
                     /translation="MNNRLLPPSSPSFLHHSEQGAQRLSELPIHINTLWNPDTCPAHL
                     LPWLAWALSVDRWDETWSEEARRQIIRDSWHIHRHKGTISAMRRAVEPFGAILSITEW
                     WENNDPPGSFRLDIDISERGLTEETRQEMERMITTVRPVSRHLAAMKMTYVARGVSRI
                     TGATLTGETIVVYPAAELTSPLSVSGDIHPGGVTYEGITVTIRPDKE"
     gene            complement(5967..6863)
                     /locus_tag="ACHLAG_004677"
     CDS             complement(5967..6863)
                     /locus_tag="ACHLAG_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFP0657778.1"
                     /translation="MPTIDLSQLPRPQIIESLDYESILKDVKAFMIDAFPENMRSAVS
                     AAIALESEPLNIIAQAIAFREMLLRQRINDAAAGCMLSHAVSTDLDNLAAIVNAKRLE
                     LKPATDTEAAIMESDSAFRLRAQSGFEGFSVAGPSAAYEYFARSASGLVADARATSPS
                     PATVIVSILSTEGDGTASPELIATVSRALNDETVRPLADRLTVQSATIVSYDIEAVLY
                     FYPGPESEPILAAARDALDTWLKTQNAIGRDVARSAIMATLHVPGVQRVELKKPAQNV
                     VISNTQSARCTSRNISAGGTDE"
     gene            complement(6850..7215)
                     /locus_tag="ACHLAG_004678"
     CDS             complement(6850..7215)
                     /locus_tag="ACHLAG_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFP0657779.1"
                     /translation="MTPVCTGMNPHGSGCISDTEHLRHSVNNILTTPRGSRIMRREYG
                     SLLPELLDAPQNDVTRLQCMSAVVMALAQWEPRIAINRVDIAWLPDGRALLAISGVLT
                     RTMQPVQMSIPLRGSNADN"
     gene            complement(7212..7793)
                     /locus_tag="ACHLAG_004679"
     CDS             complement(7212..7793)
                     /locus_tag="ACHLAG_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009486481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFP0657780.1"
                     /translation="MNAEIMRLLENMVRTGVVSEVDLKNWRIRATSGGLTTDWLRWNC
                     TRAGAFKIWLPPSPGEQVLLLCPGGSPEMAVVAGSLYSEEHPAPGTKQNEVILLAPDG
                     AEIRYDAEKGALTVKGIKTAEISAETRITLDTPAVECTKHLKARTFELTDGGTMKGDI
                     THSNGNLLSNGVTVHTHVHNGVQSGGSMTGGPK"
     gene            complement(7790..7909)
                     /locus_tag="ACHLAG_004680"
     CDS             complement(7790..7909)
                     /locus_tag="ACHLAG_004680"
                     /inference="COORDINATES: protein motif:HMM:NF016927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFP0657781.1"
                     /translation="MVSAGNAVRYPQRELLGFSEADRQKVTEIIINNLWRNTR"
BASE COUNT         1471 a         2109 c         2419 g         1914 t
ORIGIN      
        1 attgctgtta tttttaactg gccgcccgca gagatatttg caatgacgcc tgcagaggtg
       61 gtcgcctggc gtgagcgggc ggctgccaga agcggaagtc acaatcatga tgaataatct
      121 gcatatcagg gttgctctgg cggcggcaga caccctgaca caacctctca ggcgcgcccg
      181 cgaaaccgtg ggcgcgcttt ctgattccct ccgtgaaact caggactcca tccgctcgct
      241 tgaacggcag agtgaagcct ttgctgcggc acagactaaa tttgacgaag ccacccggaa
      301 aattgccgct gttcagcagc aactgaaccg tctgcagcag gcggagcgtg agggtaccgt
      361 gctcagtgaa cggcagcgtg aaatgatggc ttcgctgacc acccggcttg aacgtctgaa
      421 ccgggtgagg gatgtacatc agcgacgcct ggaggattca cgccgggagc tggagagaca
      481 cggtattgcc gcagagggca gcagtcagac catacaggca gccattcgcc ggacggcaga
      541 gtataacgaa gagctggcgc gacagcggca ggcgctcgca caggtgaccc gtgcacagca
      601 acgctatgaa caggcgcagg agcgactcgg gcggatacgt aatgccggtg cggcggcact
      661 ggctgccggt gcagcaggac tttacgctgg cgggcgtgct gttgcgccgg tgatggatct
      721 ccagcgtcat ggtgccgtca ttgcggctca ggcaggagag ggcgcgtcag atgccgtggg
      781 gtataccggg gttataaacc acatggttca taccggtatc acaccggatg cacaactggc
      841 ggctgaagct gtgggtgctg tccgctccac tctgggtggg ctgggggcgg ttagtgagaa
      901 tgaactgacc cgaatcaccc gaaaaatgct ggatatgcag gccgtgatgg ggggcgatat
      961 cccgcagcac attcaggcgg ctgccatcat gatcaaaaac ggtcttgcac gcagcagtga
     1021 cgaggcggtt gatctgatgg tggcgggcat gcagcgcatg gggccggtta tgcgcgatga
     1081 gctgcctgag atccttcacg agtattcgac ccattttcgt aacatggggc tgacagggtc
     1141 ggaaaccatg acgctccttg cggatatggc gcagcagggg aagtttgcgc tggataaaac
     1201 cggcgatgcc atcaaggagt tcagtattcg cggctccgat atgtcaaagg cgagtgctga
     1261 agcgtatgcg cttatcgggc tggatgcagc caggatgtca tcggctgtag ccagtggtgg
     1321 aacggctgca cgggctgcgc tgcagaaaac agcctcgggt ctgttgcaga tcgtcgatcc
     1381 ggctgagcgg gccaatgcgg ccatcgcact ttttggtaca ccggttgaag atctgtctgt
     1441 cgatcaaatc cctgcctttc ttggcgcgct ggcgcgggtg tctgaccgta tggctgacgt
     1501 gtctggtgtg gcggaccgga tggggcaaac cctgcgtgac aatctgagtg gtgatgtggc
     1561 gcggcttacc gggacgttca gcgcgctgag aagtgggatt atcggggaga ttacggacga
     1621 tctgcgtggg cttgtccgga tggccacagc gtggggtgag cgtctgcagg cgtgggtgaa
     1681 tgcgcatccg gaactggtgc gaatgattgc gctgacgacg ggagcggtgc tggcgttaac
     1741 cacagcgatg ggggcgttgt ccgttgcggc ctggatggtt gtcgcaccgt tactgaaact
     1801 gcgtctgggg cttgccctgc ttcgcgcgat cccggccgga ctctctgttg ccggtacgct
     1861 gtttggcgga ctgacgggtg tgatttcagc cgtgggggct gcgctggggg cgctgacctg
     1921 gccggttgtg gtggcggtgg gagccctggt cggtggtgct ctgctgatct ggcgctactg
     1981 ggatcgggtc agggcgtttg tgagcggatt tattgaaggg gtacagtcag cactggcgcc
     2041 cttcatggcg acagcctccc gttttgcgcc gctgctggat ctggtttcag gggcactgtc
     2101 ccgtattctg ggatggttta aatccctgtt aacgccagtc tcctccacgc gtgatttgct
     2161 tgagcagtgc acctctgccg ggcagacgtt cgggagcact ctggccggtg cgttagccac
     2221 ggttttttcg cccctgaaag ctctgattga cggtctggcg tgggtgctgg aaaaactcgg
     2281 tgttctgccc ggtgaagctg agcgggttaa gcgaaaactg gatgaggccc gtaaaacgcc
     2341 ggtggcatgg gagtgggatc cggaacagaa aaaaatggtt cagaaggcgt ggcactggtc
     2401 gcctcctggt ggggccagtg tgccttccgg cgcctccggt acacagcctc cgccggtggc
     2461 gctccctgcg gggatagcgc cggacggtaa ccgtaacgtt ctccgtcacc tgaaaggcat
     2521 tgaggggaat accggtggtc tgctggctga aaaccgtaaa cgtatcggac ccggagatat
     2581 tgtgtttaaa catcttccac gggcgctggc ggtccggggg cagtggcatg aacctgtcgt
     2641 tgcgccgtct tccggcggaa tgcgcgcgct gccacaggag gagattcagg tgcagcaatg
     2701 gccgacggca cgcccggcgc cacgaatgca ccagcaggaa accttcgcca gcgcctttca
     2761 gggggagata catgtgcatc ttcacggggt gagttcgcat aatccccggg aactggcgcg
     2821 catggttggt gacgctgtca gggcggaaat gaacagaatg gcacgtgccg gaaccggcag
     2881 cttccgcgat cgcgactgag gtgaaaaaga tgatgatgat ttatgggatg tttgtgtttc
     2941 agttaagtac cctgccgcat cagcaggtgg aacagagtaa acgctggcga cacgtcagta
     3001 atgaacgggt taaccgttca tcctcctggc agtatattgg ctctggtgaa gatcacatca
     3061 cgatgtccgg tgtgctctgt ccggaaatca ccggcgggga ggtgtcactg actgccctga
     3121 acaccctggc ctacacagga ttaccctggc cactgattga tggtgtcgga cagatttacg
     3181 ggatgtatgt cattacggaa atcaggacca cccgtcagga gctggatcgc tatggcatgg
     3241 cccgcaaaat tgagttcact gtcagctttc agcgatgcaa tgaagatctg cgtgagcgtc
     3301 ttcagtcggg ctcggtgggt gatctgatgg caggattaaa aaacagcgcg tcgtcggccc
     3361 tgtcgtcggc gtcctctgcg ctgtctggtc tcatgttgtg aaggtcactg ttcaggcttc
     3421 ggcttctttt ccccagccaa ccttatagtc cagaatcgcc gccgtatcgt tcagggcggc
     3481 gatctctttt ttcatgttac gctggcgaat gtgaatttcc atccctttgg caaacagggc
     3541 ttttgcggct gcatcgctga gggcaatcag ctcatcagca gaaacaggaa catcgttgtt
     3601 ctgtgcatca gtccagaaaa aatccagcgg caggatcccc gcttttgcgg cagcaacgga
     3661 aggctcaagg cgggcctgcg ttgacttccc gtagtcccat ttcctgccgt tatgttcaaa
     3721 ggtgtaactc gccgcttcca tcgcattacg ccaggcgttg atttcgtcgt ttttcaggcg
     3781 tctggcgttt tccctgtcag tgtcgttttc ctggtattca gatacagggc cgtgatttcc
     3841 tgctttcagt tcgccccaga gttttttgcc gtgtggcgag ttgtcatatt cggttgccgt
     3901 atagggcagc cagctctcct ccccttcaaa ctggatctca cagtcgatag tgccgtcacg
     3961 gttataagcg gcatttcttg ctgatttgat tgtgtgcatg agtctctccg tcaggcaatg
     4021 cgctcaaata ttccgattcc tgcgcctgca taaatgtcag cgcaggcccg ccacgtgccc
     4081 ggtggcctgc ggtttgtgtt ccagttttgc atggattcac gggggccgaa gttgccttcg
     4141 gaatgagtca ggcttatcag tctgagttta ctgcccgggc gggtgtttcc ggccgttata
     4201 tcaattttgg ctggccactc cccttcggca tccgttgccg ccagaataaa gctgcccaca
     4261 tcccagagtg tccgctgtgt ttttgcctgt gcagcctcca gtgcttgccg tgctttctgc
     4321 ccggcgtttt ctgctgccgt catggccgtc ttcacagctt ttggtgtggc agccagcact
     4381 tcactgtcac tgtccgttgc gctgcttagc tgaacaatac ctcgctctgt cagggaggcg
     4441 tcgccggtct gcgtgagggg gagtatggca acctccttcc attcagacca tggcccgttg
     4501 ccggtgaagt tacctgtcac tgtccggata aattttcgtg gtggattcca ggcggtgtat
     4561 tcctgctggc agaatcccca tgcgcccatc ctgatggata atgatccggc tgatgttacc
     4621 gggtagtgat gtgctgtagt ggcgccgttg ttgttgggct gatagtagtc accatgatgt
     4681 tcaggtctcc ccagtgtatt cagatccacg gcgagggggg ctgttggtct tgttactgtc
     4741 agcgccttaa gtgcttttat tgctttgggt gtggctgccc gggtttcatc atcactgtcg
     4801 gtttcactac tgagctgagt aaatccttta ctggtggttg tggcatccgg gtgtttaccg
     4861 ttttgttcat gctgctttat catttctgcc acgccagcct gtgttgccat ggtggttgat
     4921 gagtcggctg tcagatttac agaggcttcg ttgctcagca gaatcaccat gcggaatacc
     4981 tgttctctgc ctgagccctc atcaagctgc ggtttgtaac cttccgcaac attacccacg
     5041 gcgatgagtg tgcctttgct gtcaaacaga ccgatttcgc ggatccagaa tcctccttca
     5101 ttcgcaggaa tgatcacttc ggcggttaaa aaactggtgt tttccgcgtc acggctgacc
     5161 ctgctgacct cgccccgcca gacctcttta acgagagcgg tttgcgtggc cagtggttct
     5221 gtgggtttac cgttaccgtc tccgacggcg atatgtgtca gcgtgatctt ttctccatcc
     5281 ggtaacaccg cagctgcgag ggctgctgtg ccggcatttg tgataatggt tttgtatttt
     5341 gcggccatca ttactccttg tccggtctga tggttactgt gatgccttca taggtgactc
     5401 cgcccgggtg gatgtcacca ctgactgaca gcgggcttgt cagttctgct gccggatata
     5461 ccacgatggt ttccccggtc agcgtggccc cggttatgcg ggatacaccc cgtgcaacgt
     5521 aggtcatttt catggcggca aggtgtcggc ttaccgggcg aacggtggta atcattcgct
     5581 ccatctcctg ccgggtttcc tctgtcagtc cgcgctccga tatgtcaata tccagcctga
     5641 atgaccccgg cggatcgttg ttctcccacc attctgtaat actcagtatc gcgccgaagg
     5701 gctccacagc ccgacgcatg gcgctgatgg tgcctttgtg gcggtgaata tgccacgagt
     5761 cacggatgat ctgacgacgt gcctcttcac tccatgtttc atcccagcgg tcaacggaaa
     5821 gcgcccaggc aagccatggc agcagatggg ctgggcaggt gtcggggttc cagagggtgt
     5881 tgatatggat cggcagctct gacaggcgct gagcgccctg ttccgaatga tgcaggaagg
     5941 agggcgatga tggcggcagc agtcggttat tcatcggtgc cacctgcaga gatgttccgg
     6001 gatgtgcagc gggctgactg ggtgttactg atcaccacat tctgcgctgg tttcttcagc
     6061 tcgactcgct gaacgcccgg tacgtgtagc gttgccataa tggcagagcg ggccacatca
     6121 cgccctatgg cattctgagt cttcagccat gtgtcaagtg catctctggc ggcggcaaga
     6181 atgggttcag attccgggcc aggataaaaa tagagtacgg cctcaatatc gtaactgacg
     6241 atggtcgcac tctgtaccgt gagtctgtcg gccagggggc gcacagtttc atcattgaga
     6301 gcccggctga cggtcgcgat taattccggt gacgctgtgc cgtctccttc cgttgagaga
     6361 atggagacaa tgaccgtcgc aggtgacgga ctggttgccc gtgcatcggc aacaagaccg
     6421 ctggcactgc gggcaaaata ttcatatgca gcagaggggc ctgcgacgct gaaaccttca
     6481 aatccggatt gtgcccgcag acggaacgcg ctgtcgctct ccatgatggc agcttctgta
     6541 tcagtagcag gtttcagttc aaggcgtttc gcattcacga tagcggcgag attatccagg
     6601 tctgtggata cggcatggct gagcatgcac cccgccgctg catcgttgat ccgctgacgg
     6661 agcagcattt cgcgaaaggc gatggcctga gcaatgatgt tgagtggctc tgattccagc
     6721 gcaatggctg cgctgacggc actgcgcatg ttttcaggga aggcatcaat cataaaggcc
     6781 ttcacgtctt tcagtatgct ctcataatcc agtgattcaa tgatctgcgg acgtggtaac
     6841 tgagacaggt caattgtcgg cattactccc cctcagcgga atgctcatct gtacgggctg
     6901 cattgtgcgg gtgagcacgc ctgagatggc cagcagagcg cgtccgtcag gcagccaggc
     6961 gatatccacc cggttaatgg cgatcctggg ctcccactgt gcgagcgcca taaccaccgc
     7021 gctcatgcac tgcagacgcg tcacgtcgtt ttgtggcgca tcgagcagtt caggtaacag
     7081 gctgccatac tctcttcgca tgatgcgact gccgcgcggg gtggtcagaa tattgtttac
     7141 agagtggcgg aggtgttctg tgtcactgat gcatccgcta ccatgcgggt tcatgcctgt
     7201 gcataccggt gtcatttcgg cccccctgtc atgcttccgc cggactgaac accgttatga
     7261 acgtgcgtat gcacggtaac accatttgag agtaagtttc cgttactgtg tgtgatatcc
     7321 cctttcatgg tgccaccgtc agtcagttca aacgtcctgg ccttcagatg ttttgtgcac
     7381 tctacggccg gtgtgtccag ggtaatacgt gtttccgcag agatctctgc ggttttgatt
     7441 cctttcaccg tcagcgcgcc tttttctgcg tcatagcgta tctcggcacc gtcaggtgca
     7501 aggagtatca cttcattctg ttttgtgccg ggcgccgggt gttcttcgct gtagagactg
     7561 ccagccacaa cggccatttc aggactgccg cccgggcaga gcaacagcac ctgttcaccg
     7621 ggggaggggg gcagccagat tttaaacgcc ccggcgcgtg tgcagttcca gcggagccag
     7681 tccgttgtca gtccgccgct ggtagcgcga atgcgccagt ttttcagatc cacctccgaa
     7741 acgacgccgg tgcggaccat gttttccagc agtcgcatga tttcagcatt caacgggtgt
     7801 ttctccacag gttgttgata atgatttccg tcactttctg tctgtcggct tcactgaatc
     7861 ccagcagttc gcgttgcgga tagcggacgg cattaccggc agagaccagt gac
//
LOCUS       DAZGVQ010000207         7883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000207 DAZGVQ010000000
VERSION     DAZGVQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.209"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(207..283)
                     /locus_tag="ACHLAG_004681"
     tRNA            complement(207..283)
                     /locus_tag="ACHLAG_004681"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(247..249),aa:Arg,seq:tcg)
     gene            complement(293..368)
                     /locus_tag="ACHLAG_004682"
     tRNA            complement(293..368)
                     /locus_tag="ACHLAG_004682"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(333..335),aa:Ile,seq:cat)
     gene            complement(409..1467)
                     /locus_tag="ACHLAG_004683"
     CDS             complement(409..1467)
                     /locus_tag="ACHLAG_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFP0657782.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMREHFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLISYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPDESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLVSTYSELNRQYTELQSEYKHLRRYFGVTVRVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMVLGRRATGVELET
                     ERFEQTVREVQDLASQNG"
     gene            complement(1618..1815)
                     /locus_tag="ACHLAG_004684"
     CDS             complement(1618..1815)
                     /locus_tag="ACHLAG_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP0657783.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            complement(2258..2941)
                     /locus_tag="ACHLAG_004685"
     CDS             complement(2258..2941)
                     /locus_tag="ACHLAG_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP0657784.1"
                     /translation="MNNQYLQFVREQLMIATADLSGATKGQLEAWQENALFDTGRYRR
                     KKIRYRDEVTGKMITRDNQPIPGKQSLAKGSSIALVSPVEFATSSWRRAVLELEEHQK
                     AWLLWCYSGNVCWEDQVTITQWVWGEFKAQAGTRKIAGKTLDRLKKLIWLAAQDVKSE
                     LAGRETYEHQELAVFIGVAKSTWTKTHRPHWLAMRGSAEMLDRDALISVMRVRSRQKS
                     INLEPYLAK"
     gene            complement(2938..3297)
                     /locus_tag="ACHLAG_004686"
     CDS             complement(2938..3297)
                     /locus_tag="ACHLAG_004686"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0657785.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVNQQ
                     RLKLSLSGRLVIRIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGL
                     PVPGGRLGIKITELECA"
     gene            complement(3310..4359)
                     /locus_tag="ACHLAG_004687"
     CDS             complement(3310..4359)
                     /locus_tag="ACHLAG_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0657786.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHCGRVLVEPEPKSMRA
                     LPSGFVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGVLSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYSTGAMVLCWHCDNHLREQTSDSLELLAQQNLAAWMIDVIRHVMNGAQERE
                     LSLAELSWWAVCNQVADALPESVLRRSLGLPVEKIRSVYSESDIIPGEQTATSILKQR
                     TKNIAPPLHAHQPQTTPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKFTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLEFEKKYGSQVEL
                     IFHFLDHAFATGVLG"
     gene            complement(4361..4633)
                     /locus_tag="ACHLAG_004688"
     CDS             complement(4361..4633)
                     /locus_tag="ACHLAG_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657787.1"
                     /translation="MAQHIKSHNSEAVPAIKRGRHSHVFKTHWYQHPPCTEEQAEWLI
                     QCYRRRGYEVKKALSLNYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            4806..6032
                     /locus_tag="ACHLAG_004689"
     CDS             4806..6032
                     /locus_tag="ACHLAG_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP0657788.1"
                     /translation="MSGTITKISTGENVLSAAIHRIEWLFETFNEVCLSFSGGKDSTV
                     LFHLVAEVASRKKRRFSVLFIDWEAQYQCTINHIQKMREMYRHVTDNFYWVALPLTTV
                     NGVSQFQPEWICWEPGVEWVRQPPDDAITDMSYFPFYRYAMTFEEFVPAFSSWFAGNR
                     CGVGILTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPDGFYYTMYPLYDWKTNDI
                     WTYNSRTRAIYNPLYDLMYRAGVSLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMCE
                     RVSGAASGALYANESGAYFALRKRITKPAHHTWRSYAMFMLDVMPERTAEHYRNKIAV
                     YLRWYQTRGFPDDIPDEQENDLGSRDIPSWRRICKTLIKNDFWCRTLSFSPNKPRHYE
                     RYLQRMKERRKEWGIL"
     gene            6017..6661
                     /locus_tag="ACHLAG_004690"
     CDS             6017..6661
                     /locus_tag="ACHLAG_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFP0657789.1"
                     /translation="MGDSVTPEMDVLSNMIRQYFSREQPEEESIRALNHLRRVLHEVS
                     PFAQEPVDCVLWVKADEVVANDYNPNVMAPSEKKLLKQSLEKDGFTQPVVVSEEKNHY
                     LVVDGFHRQLLGRESVTGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQIDS
                     VSDIVRDLSRLGWADERIGTELGMDQDEVLRLKQISGLTELFQEVDFSPAWTVK"
     gene            complement(6827..6931)
                     /locus_tag="ACHLAG_004691"
     CDS             complement(6827..6931)
                     /locus_tag="ACHLAG_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657790.1"
                     /translation="MSDDISLAMEGALAVIVVVGVYCLVVLLMDRLGS"
     gene            complement(7047..7610)
                     /locus_tag="ACHLAG_004692"
     CDS             complement(7047..7610)
                     /locus_tag="ACHLAG_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP0657791.1"
                     /translation="MLTIQHWRETYGPGSNVVLPAEEAEELARIALASLAAVSDERAA
                     YELFMEKRFGESVDRRRAKNGDREYMAWDMALGWIIWCHRAAMLQAGNFRENKGSSTN
                     NFREISETSTNYPVTPDGWISCSERMPDDGQHVIILCDGAFVLYAQYRDGEFFDVVRN
                     GDEFFETQSRNVTGWMPLPEPPQEVRQ"
     gene            complement(7631..>7883)
                     /locus_tag="ACHLAG_004693"
     CDS             complement(7631..>7883)
                     /locus_tag="ACHLAG_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657792.1"
                     /translation="IISVEDLPPGWGLLHVVNGRVRKVHGWPRGNCCWGNPDDKPFTG
                     NKQVECDYMLSALRRMELRGHLNEIYDGVIVNKKEGNAA"
BASE COUNT         1965 a         2086 c         1834 g         1998 t
ORIGIN      
        1 taactatcat ctccggatca gcatgtagtt tttatttttc cggatgatat atgccgcaat
       61 aatacccctg catacagatg cctgcaaata tctacgaagc atccggcgag aataaacaag
      121 gaaatctgag actatcttat atgatagcct gttgctcaaa agacaatgat tcactcatca
      181 gagctaacaa cgcatgatgc agataatgga ccgccatcga ggactcgaac cccgcgcagc
      241 cagctccgaa ggctggcgct ctatcccgat gagctaatgg cggtatgtga tatggtggcc
      301 cttgctggat ttgaaccagc gacctggcga ttatgagtcg ctcgctctca ccactgagct
      361 aaagggccgg gcgcaggata ataacggtac gtaactaatc ctgcaatatc atccgttctg
      421 gctggctaaa tcctgaactt ccctgaccgt ctgctcaaaa cgttcagtct ccagctcaac
      481 gcctgtagca cgacgcccca acaccatcgc tgctttgact gtcgaacccg accccatgaa
      541 gaaatctgca accaggtcac ccggacgact gctcgcactg attatctgct gcagcatttc
      601 tgccggtttt tcgcacggat gtttcccggg atagtactgc accggtttat gcgtccacac
      661 atcggtgtac ggcacccgca ccgtcacacc gaaataccgc cgcagatgct tatattcact
      721 ctgcagttcc gtatactgcc ggttcagttc actgtatgtg ctgaccagtt ggtgatgtgg
      781 cttttccagt tctccgcgct gatgcttctc ttctgccacc cgggcaaaca gcgcctgtaa
      841 tttcagataa tcgctttcgt ccggtaactg ccactggctg gcactgaacc agtgcgacac
      901 catatttttc tttcctgtgg catccactat ctgttttgcc gttatcccca gggcagcacg
      961 cgcatcacga aagtaagaaa tcagcggggc catcacatgc tgtttcagtg ccctgccctt
     1021 cgcctcatac ccggcatctt tcgggcgata cggcccctga taatgttccg cgaacagaat
     1081 gcgctctgtg gcggggaaat acgccctcag gctttccttg ttgcacccgt tccagcgtcc
     1141 ggacggcttt gcccagataa tatggttcag cacactgaag tgttcacgca tcatgatttc
     1201 gatatcagat gccaggcgat ggccacagaa caggtaaaga cttccggcag gtttcagcac
     1261 ccgccagaac tgcgcaagac actggtccag ccacttcagg taatcatcgt cgcccttcca
     1321 ctggttatcc cagccctcag gcttcacttt aaagtacggc gggtccgtga ctatcaggtc
     1381 aacagaattt tcgggtaacg accggataaa ttcaaggcag tcggcgttga ttaactcaca
     1441 actggatatt tttacagtat taagcatgga tcattaagcc tgtctctgat aggctcattc
     1501 tgcttttgcg caaagcagtg ggcctgaggt ttgcttgtga acccaacgca tgagcagatg
     1561 gctggggggt gcccctaaca cccaccagcc gcccatttac cacaaataaa aaagccttca
     1621 ctgcggaagg cgtctgtaac aaccgaactg atagtctgcc agacccgcca taaccagctg
     1681 ggtcagtatt aactggcagc gttcgcgtga aaggtaagta ttctgcgcta tctccccgac
     1741 tgtcgccggt tcggtgacgc ttaattcatt aaacaccact ctggcggttt ctgtcatatc
     1801 ctgctgtttc agcatgtctt tttccctttt ccggttaacg tgacacacca ataactcttg
     1861 tcgaaaaagc cagcaagata aaaggtcgct attcacgacc accagcgcgt ttaacgtcct
     1921 gtgccgtttt tcgggcataa aaaacccgcc agtggcgggg atcttttgat aaaaacgaca
     1981 aaggcaccat taaggtgcct tttacacagg gcagcaggct tgtcatcacc ttattgctac
     2041 tcctggcttc cagttcggaa gtctcacaag tcccatcagg gtacagcgac agtatcagtc
     2101 aacactctat gattctgctg aacaggcagt ttcgcactgt acattataac ctgttaagcc
     2161 aacaaaaaac ccgctcggcg gcggggtttg cttactttgc catcgcgaac aaaatctgca
     2221 aaatatcaga tttacacgga atatatgctc ttcagttcta ttttgcaaga tatggttcta
     2281 aattaatcga cttttgccgt gaacgtaccc gcattactga aataagagca tccctgtcca
     2341 gcatctctgc actaccccgc atagccaacc agtgaggcct gtgtgttttt gtccatgtag
     2401 atttagcaac tcctatgaac accgctaact cctgatgttc ataggtctca cgccctgcca
     2461 gctcactctt cacatcctgt gccgccagcc aaatcagttt cttcagacga tccagcgttt
     2521 tccctgcaat ttttctggta ccagcctgag ctttaaactc accccatacc cattgtgtta
     2581 tagtgacctg atcttcccag caaacatttc cactgtagca ccacagtaac cacgccttct
     2641 gatgttcttc cagttccaga acggcacgcc gccacgatga tgttgcaaac tcaaccggac
     2701 tgaccagcgc aattgatgag cctttcgcca gtgattgttt acccgggatc ggttgattat
     2761 cccgcgtgat catttttccg gttacctcat cgcgataacg aatttttttg cgtctgtaac
     2821 gccctgtatc gaacagggca ttctcctgcc aggcttccag ctggcctttt gtcgccccac
     2881 tgagatccgc agtggcaatc atgagttgct cacgaacaaa ctgtaaatac tggttattca
     2941 tgcgcactcc agttctgtga tttttatccc cagccgcccg ccaggaacag gcaggccacg
     3001 cacaatattg atttcatcaa actgctcatc gtccattaac aaccccgcat gcgtaagcgc
     3061 atccagcggc gctttcagaa tattgtccag gtcacggcgg cgcttatccg gtggctctgc
     3121 aataatcctg attaccagcc ttccggacag gcttaatttc agccgctgct ggttaacaat
     3181 aagcgccaca gcacggcgat agcgctttcc ctcctccgag ataaaatatg tgctgccacg
     3241 acgacgccag taggtgttca ccgttggcgg gtaaggtaaa accaaatcta tgagcatcag
     3301 tcacctcttt tacccgagca cgccagtcgc aaaggcgtga tcaagaaaat gaaaaattaa
     3361 ctcaacctgg gacccgtact ttttctcaaa ctccagcggg tcagcatgaa gttcgttgtg
     3421 gtgctcccgg cacaacggta gtgtgaaaat atcgtgggat tttgtcccca taccgccctg
     3481 cccatggcca atcaagtgat gcggatcatc agctggcttt ccacagcatg cacacggctg
     3541 cgtcttaacc cagcgcgtga acttttcatt aacccagcga cgacgtttag gccgtttcat
     3601 gaaagattcc ggggactccg gatcaacggc gatgcttact accgtctttt cctgtggcgt
     3661 ggtttgtggc tggtgggcgt gaagcggtgg cgctatgttt tttgtccgct gcttcaggat
     3721 gctggtggcg gtctgctctc ccggtatgat gtcgctctca ctgtatacgg agcgaatttt
     3781 ttccaccggt aatcccagcg aacgacgcaa tacagattcc ggaagcgcat cagccacctg
     3841 attgcagacc gcccaccagg ataattcagc cagcgataat tcacgctcct gtgcgccatt
     3901 cattacgtga cggatgacgt caatcatcca ggcggccaga ttctgttgag caagtagctc
     3961 cagtgaatca gatgtctgct cccgcaggtg gttgtcgcag tgccagcaca acaccatcgc
     4021 gccggtactg taacggtgaa tgacggtttc gctgtgatga taatcgccgt gtggccactg
     4081 gcaggatttc acgtgacgta ataaccagtc agacagtaca ccagcgccac ccgcagcacg
     4141 gatcacccgc tcatcgctga aaaatggcag taatgattta tcttccgcca gcggctggcg
     4201 aacggcaggt acaaacccgg acggcagagc acgcatgctt ttcggttccg gctccaccag
     4261 caccctgccg caatgaaaaa caggcagtga ttcgcgtccg ggcttaagga ccaccaggcc
     4321 aagttccggc accagaacag gtcgaagtaa tacccgcacg ttacctccag atacgttgct
     4381 ggaatgtgcg ggacggacgc ggtggacgtt cggaataagg gagcctgaca gagattatcc
     4441 agtgacgata attgaggctg agggctttct taacctcgta tccgcgcctg cggtaacact
     4501 gaatcagcca ttcggcctgt tcttcagtgc atggaggatg ctggtaccag tgggttttaa
     4561 atacgtgcga atgccgcccc cgcttaatgg ccgggacggc ttcagaattg tgggatttta
     4621 tatgttgcgc catcgggttc tccggtgaca cagcaggtgc cagttgttca ggctggctgg
     4681 atgattttaa ccagaatgcg ataaaaagtg tataagacca tcacatattg cattcatgca
     4741 aaatgattgt tacataatat tacttaataa gtataggttt taccgtttta tcattagaag
     4801 attgtatgtc aggaaccata acaaaaatca gcacagggga aaatgttctg tcagcagcaa
     4861 tccatcgcat tgaatggttg ttcgaaacgt ttaatgaggt ctgcttgtct ttttctggtg
     4921 gcaaagactc tactgtgctt ttccatcttg tggcagaagt cgccagtaga aaaaaacgtc
     4981 gtttttcggt tttattcatt gactgggaag cccaatatca gtgcacgatt aatcatatcc
     5041 aaaagatgag ggaaatgtac cggcatgtga cagacaattt ttactgggta gcgcttcctc
     5101 tgactacggt aaatggcgtc tctcagtttc agccagaatg gatatgttgg gagccaggtg
     5161 tagaatgggt tcgtcagcca ccagatgacg ctattacaga tatgtcgtat ttcccatttt
     5221 atcggtatgc catgacgttt gaagaatttg ttccggcatt ttcttcctgg tttgccggta
     5281 accgatgtgg agtgggaata ctaactggtg ttcgtgctga tgaatcgctc aatcgcttta
     5341 tgggactggt gtctcagcgc aaactgagat atgcagatga taaaccctgg accaccgctt
     5401 cacccgatgg tttttactac accatgtatc cattgtatga ctggaaaaca aatgatatat
     5461 ggacgtataa ctcaagaacc cgcgccatct acaatcccct gtacgaccta atgtaccgtg
     5521 ccggagtgtc gttacgcaac atgcgtgttt gtgagccctt tggcccggaa cagcgtaagg
     5581 ggctgtggct ttaccatgtt ctggagccgg aaacctgggc caggatgtgt gagcgagtgt
     5641 caggggctgc cagtggcgct ctttatgcca atgaaagcgg tgcctatttt gctctgcgta
     5701 aacgaatcac aaaaccggct catcatacct ggcgcagcta cgcaatgttc atgctggatg
     5761 ttatgccaga aagaacagca gaacattacc gtaacaaaat tgcagtctac ctgcgctggt
     5821 atcagacgcg gggcttcccg gatgatatcc cggatgaaca ggagaatgac ctggggagcc
     5881 gggatatccc gtcctggcga cgtatctgta agacgctcat aaagaatgac ttctggtgcc
     5941 ggaccctctc cttcagtccg aacaaacccc ggcactatga gcgttatctg caacgaatga
     6001 aggaaaggag gaaagaatgg gggattctgt gacgcctgag atggatgtac taagcaacat
     6061 gattcgtcag tacttcagtc gggaacagcc cgaagaagag tccatacgag cccttaacca
     6121 tctgcgcagg gtactgcatg aagtcagtcc ttttgcacag gagcctgtag actgtgtgct
     6181 gtgggtgaaa gcggatgagg tggtagccaa cgattataac cccaatgtca tggcaccaag
     6241 tgagaagaaa ctgctgaagc agtcactgga aaaagatggt ttcacacaac cggtggtggt
     6301 gtcagaggag aagaatcatt atctggtggt ggatggtttt catcgtcagt tattgggcag
     6361 agaatcagtt acaggaaaac gtctgagggg ctggctgcct gtagcctgta ttaatccaga
     6421 gagaaaaggg caagccgcac gcattgccgc aaccattcga cataaccggg ctagggggaa
     6481 acaccagata gactcagtgt ctgatattgt aagagatctt tcacgcttgg gatgggcaga
     6541 tgaacgcatt ggcactgagt tgggtatgga tcaggatgaa gtactgagac ttaaacaaat
     6601 tagcgggcta actgagttat ttcaggaagt ggatttcagc ccggcatgga cagttaaata
     6661 gcaagctcga cttactcccg gcaaggcaac aaaatgataa ctaatacaac tcaagggcga
     6721 ttaatatcgc cccttttact gctcaatttt cgaccgatac atctacttga ctctcgtttt
     6781 actccccctt tttacccgat atggaaattc ccatatcgta atgaattcag ctccccagac
     6841 gatccatcaa aagcacgacc aggcagtaaa cacccacaac aacaataacg gccagagcgc
     6901 cttccattgc cagtgagata tcatccgaca tattccctcc tttggtgtga atcccggcga
     6961 acgtttttac ccccaccgac aaataacata tattaaaaaa gcgataacca tagcaacgcc
     7021 tgtaattgca aatgcttcag gccagttcat tggcgcacct cctgcggcgg ttctggtagc
     7081 ggcatccagc cggttacatt gcggctctgt gtttcgaaaa attcatcacc attacggact
     7141 acatcaaaaa actcaccgtc tcgatattgc gcataaagaa cgaatgcgcc atcacataaa
     7201 ataattacgt gctgaccatc atccggcatt cgctcactac agcttatcca accatccgga
     7261 gttaccggat agttggttga cgtttccgag atttcccgaa aattattggt tgacgacccc
     7321 ttattttccc gaaagtttcc agcctgaagc atggcggcgc ggtgacacca gataatccag
     7381 ccaagcgcca tatcccatgc catgtattct ctatcgccat tttttgctct gcggcgatct
     7441 acagattctc cgaaacgctt ctccataaat aattcatagg ctgctcgttc atccgatact
     7501 gctgccagtg atgccagcgc aatccgtgcc agctcttccg cttcttctgc tggaagcaca
     7561 acgttgctac ccggtccgta tgtttcgcgc caatgctgga ttgtcagcag tcaatctttg
     7621 gtaatagtgg tcatgccgcg tttccttctt tcttattaac aattacaccg tcatatattt
     7681 cattaaggtg ccctctcaac tccatgcgcc ttaatgcaga taacatgtaa tcgcattcaa
     7741 cctgcttatt tccagtaaat ggcttatcgt caggattacc ccaacagcaa ttacccctgg
     7801 gccatccatg tactttccgt actcttccgt taacaacgtg aagtaatccc cagccgggag
     7861 gtaaatcctc aactgaaata att
//
LOCUS       DAZGVQ010000208         7816 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000208 DAZGVQ010000000
VERSION     DAZGVQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.210"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(184..447)
                     /gene="cedA"
                     /locus_tag="ACHLAG_004694"
     CDS             complement(184..447)
                     /gene="cedA"
                     /locus_tag="ACHLAG_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFP0657793.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            630..2891
                     /gene="katE"
                     /locus_tag="ACHLAG_004695"
     CDS             630..2891
                     /gene="katE"
                     /locus_tag="ACHLAG_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFP0657794.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2938..3696)
                     /gene="chbG"
                     /locus_tag="ACHLAG_004696"
     CDS             complement(2938..3696)
                     /gene="chbG"
                     /locus_tag="ACHLAG_004696"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFP0657795.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVVRFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDSSHRRERSLEVMCHPAFVDNLIRKSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(3709..5061)
                     /gene="celF"
                     /locus_tag="ACHLAG_004697"
                     /gene_synonym="chbF"
     CDS             complement(3709..5061)
                     /gene="celF"
                     /locus_tag="ACHLAG_004697"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP0657796.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5166..6008)
                     /gene="chbR"
                     /locus_tag="ACHLAG_004698"
     CDS             complement(5166..6008)
                     /gene="chbR"
                     /locus_tag="ACHLAG_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFP0657797.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6016..6366)
                     /gene="chbA"
                     /locus_tag="ACHLAG_004699"
     CDS             complement(6016..6366)
                     /gene="chbA"
                     /locus_tag="ACHLAG_004699"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFP0657798.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6417..7775)
                     /gene="chbC"
                     /locus_tag="ACHLAG_004700"
     CDS             complement(6417..7775)
                     /gene="chbC"
                     /locus_tag="ACHLAG_004700"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFP0657799.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
BASE COUNT         2119 a         2039 c         1729 g         1929 t
ORIGIN      
        1 atcatgatta gcaaaactta accattttaa aataaataaa caattaaaga aaaaagatca
       61 cttatttata gcaatagctc gtcaaaggca gctttttgtt acaggtggtt tgaatgaatg
      121 tagcaacgaa atgcagaatt tcaggtcatg taactcccgg caaaaccggg aggtatgtaa
      181 tccttactca gtcacttccc cttcctggcg gatctgattt gcccaacgtt gggcagattc
      241 aggcacagta aacgccggtg agcgcagaaa tgactctccc atcagtacaa acgcaacata
      301 tttgccacgc agcatccaga catcacgaaa cgaatccatc tttatcgcat gttctggcgg
      361 cgcgggttcc gtgcgtggga catagctaat aatctggcgg ttttgctggc ggagcggttt
      421 cttcattact ggcttcacta aacgcatatt aaaaattaga aaaactgtag tttagccgat
      481 ttagcccctg tacgtcccgc tttgcgtgta tttcataaca ccgtttccag aatagtctcc
      541 gaagcgggat ctggctggtg gtctatagtt agagagtttt ttgaccaaaa cagcggccct
      601 ttcagtaata aattaaggag acgagttcaa tgtcgcaaca taacgaaaag aacccacatc
      661 agcaccagtc accactacac gattccagcg aagcgaaacc ggggatggac tcactggcac
      721 ctgaggacgg atctcatcgt ccagcggctg aaccaacacc gccaggtgca caacctaccg
      781 cccctgggag cctgaaagcc cctgatacac gtaacgaaaa acttaattct ctggaagacg
      841 tacgcaaagg cagtgaaaat tatgcgctga ccactaatca gggcgtgcgc atcgccgatg
      901 atcaaaactc actgcgtgcc ggtaaccgtg gtccaacgct gctggaagat tttattctgc
      961 gcgagaaaat cacccacttt gaccatgagc gcatcccgga acgtattgtt catgcacgcg
     1021 gatcagcagc tcacggttat ttccagccat ataaaagctt aagcgatatc accaaagcgg
     1081 atttcctctc agatccgaac aaaatcaccc cagtatttgt taggttctct accgttcagg
     1141 gtggtgctgg ctctgccgat accgtgcgtg atatccgtgg ctttgccacc aaattctata
     1201 ccgaagaggg tatttttgac cttgttggca ataacacgcc aatcttcttt atccaggatg
     1261 cgcataaatt ccccgatttt gttcatgcgg taaaaccaga accacactgg gcaattccac
     1321 aagggcaaag cgcccacgat actttctggg attatgtttc tctgcaacct gaaactctgc
     1381 acaacgtgat gtgggcgatg tcggatcgcg gcatcccccg cagttaccgc accatggaag
     1441 gcttcggtat tcacaccttc cgcctgatta atgccgaagg gaaggcaacg tttgtacgtt
     1501 tccactggaa accactggca ggtaaagcct cactcgtttg ggatgaagca caaaaactca
     1561 ccggacgtga cccggacttc caccgccgcg agttgtggga agccattgaa gcaggcgatt
     1621 ttccggaata cgaactgggc ttccagttga ttcctgaaga agatgaattc aagttcgact
     1681 tcgatcttct cgatccaacc aaactgatcc cggaagaact ggtgcccgtt cagcgtgtcg
     1741 gcaaaatggt gctcaatcgc aatccggata acttctttgc cgaaaacgaa caagcagcat
     1801 tccatccagg tcatattgtt cctggtctgg acttcaccaa cgatccgctg ttgcagggac
     1861 gtttgttctc ctataccgat acacaaatca gtcgtcttgg tgggccgaat ttccatgaga
     1921 ttccgattaa ccgtccgacc tgcccttacc acaacttcca gcgtgatggc atgcatcgta
     1981 tggggatcga cactaacccg gcgaattacg aaccgaactc gatcaacgat aactggccgc
     2041 gcgaaacacc gccgggaccg aaacgcggcg gttttgaatc atatcaggaa cgcgtggaag
     2101 gaaataaagt tcgtgagcgc agtccatcgt ttggcgaata ttattcccat ccgcgtctgt
     2161 tctggctaag tcagacgcca ttcgagcagc gccatattgt cgatggtttc agttttgagt
     2221 taagcaaagt cgttcgtccg tatattcgtg agcgcgttgt tgaccagctg gcgcatattg
     2281 atctcactct ggcccaggcg gtggcgaaaa atctcggtat cgaactgact gacgaccagc
     2341 tgaatatcac cccacctccg gacgtcaacg gtctgaaaaa ggatccatcc ttaagtttgt
     2401 acgccattcc tgacggtgat gtgaaaggtc gcgtggtagc gattttactt aatgatgaag
     2461 tgagatcggc agaccttctg gccattctca aggccctgaa ggccaaaggc gttcatgcca
     2521 aactgctcta ctcccgaatg ggtgaagtga ctgcggatga cggtacggtg ttgcctatag
     2581 ccgctacctt tgccggtgca ccttcgctga cggtcgatgc ggtcattgtc ccttgcggca
     2641 atatcgcgga tatcgctgac aacggcgatg ccaactacta cctgatggaa gcctacaaac
     2701 accttaaacc gattgcgctg gcgggagacg cgcgcaagtt taaagcaaca atcaaggtcg
     2761 ctgaccaggg tgaagaaggg attgtggaag ctgacagcgc tgacggtagt tttatggatg
     2821 aactgctaac gctgatggca gcacaccgcg tgtggtcacg cattcctaag attgacaaaa
     2881 ttccggcgta aatatggata aggtcacgtc ccctggggag tgaccttacc accttactta
     2941 cacatcaaga taactaccca accgataacc acgctcagca attgcatatt tcaacgacgc
     3001 tgatgtcagc acttccagct ccgttaaccg tgggaaacag taagcactct tgcgaatcaa
     3061 attatcgaca aacgccggat ggcacatgac ttccagcgag cgttccctgc gatggctgga
     3121 atcatccagc acttgcagga acagtgcctc gctaatctct tcaccataaa acgcgctact
     3181 gaatccctgc gaactgcgca gattagccgg taaatcacca gcgttagata gcggctgacg
     3241 gtcgatgcgc aacgcaatcc cctcttcagc cgcaaacctg acaacaatcg ggaaaatctg
     3301 cgggaacata tgcacatgat gatggctatc aagatgcgta ggtttgcgtc caaatagctc
     3361 aatgaaacgc aaatactgac tggcaagctc ctgagtaatt tcttccagcg gtaaagcatc
     3421 ttcttctgcc aactgccaga tccattttcc cagcacacca tcgcgggtta accccggcat
     3481 agctgtcagt ggcttgccca tagtaaggac aaagtgcatc cctatggcca gactcggttc
     3541 atcacgactc aactgcaccg catggtcaat agcctgccca ttcaccagcg ccgtcgtcga
     3601 tgtgacaatc ccattgcgac aggcctcgat aatgccgtag ttctgacctt tgcttaagcc
     3661 aaaatcatcg gcattaacaa tcagtaagcg ttccataatc agcctcggtt aatgtgcttt
     3721 tttaagctct gcgatgcagt cggcaaaatt tggcagccat ttctcgtgcg ccagaatcat
     3781 ctcgcgtgcc agcagctcag catcgcgatc ggaatgcacc aacggactaa ggtttagcgc
     3841 cagtaacaca tcgttaaatt ctccgctaag tgcggcgttg ctggcagcaa tctcgaagcc
     3901 tttaatggtg tgaatcagcc ccatcacttt atcatcgaaa tgcgtaatgc gcggatgtgg
     3961 cgtcgcgcca tcgcgcccca gcttacaggt catttctacc gcccaatctg ccggaatatt
     4021 atcaatatgc ccatgatgcg ggatattaac gtaatgttca gcttgcttgt cgttgtagat
     4081 agcgttgatc acttcgcacg ctgcatcaga gtaataagcc ccaccgcgct gttccagttc
     4141 tttcggctta actttcaact ccggattttt atacagctca aaaagttgtt tctcgacttt
     4201 ctgtactacc tgtgctcgtg cgccgccttt gtagtattcg cccatttcaa tagccagcat
     4261 ctctttctgc ttgaagtaat acagcagata agaacatggc agcagattta acgaacgaat
     4321 taagccctca ctaaatggca gatcgaaaat attttttaca gaggacgctt ttaactgccc
     4381 tgacgccaca ccatcaagca attcggcaaa gcgcgacttg ccatttacca gcacatcctt
     4441 aatgaacacc atatggttga ggccgaacag atcgatagat aaatcatcgc tgtctttcag
     4501 catcagaaca tcgcgaataa acatcttcat gccgatcgga atattacaca cgccgataaa
     4561 gcgtttaaat ccggtatgac gataaacggc ttcagtgacc attcccgccg ggttagtgaa
     4621 gttaatcacc catgcattcg gacaaagttc ttcgacatct tttacgatgt caaaaatcac
     4681 cggaatggta cgcagacctt taaacagacc gcccgcgccg ttggtttcct gaccaagata
     4741 accatgactt aatggaatac gttcatccag ttcgcgcgcc ggtaattggc ctacgcgcag
     4801 ttgggtagta acgaaatcag catctttcaa tgcttcgcgg cgatccagcg ttttataaag
     4861 cttcatcggg acgccagcgt tatcaatcat ccgttggcag agttcaaaaa tgatatccag
     4921 tttctcttta ccatcttcga catccaccag ccataattcg ctgaccggca attcgtgata
     4981 acgcttaata aatccttcca gtaactccgg ggtatagctg ctcccgccac caatagtgac
     5041 gacttttaat ttctggctca taatttctcc cttcagtaca gaatactgat atctggcata
     5101 tctgcccccc cggacataaa taatccagca acagtacaga tatgtgaatt gtcaggtata
     5161 acgacttact gattaaattc agtcaatttc ttacgatagc tcttaggcgt aaaggacgtt
     5221 aatttcttaa acgttttaat aaacaaactc gggctactat aaccggcctc aaacgcaata
     5281 tccgtcacgg aatagttggt catttccagt tgttttttgg caaaattaat acggatctca
     5341 ttaataatct gcattggtgt tttgccataa tatcgttgag tcgctcgcgt caaatattcc
     5401 tgtgatttgg ctgacaacgt caccatattc tccagcgccg attcactaaa ctgctcttta
     5461 tcatgcatct tttctaccgt acttttcagc cactgcggta catcatcaat cacctgttct
     5521 tcgcggtaat gacgtaaacg gttaatgaca taaaaagtaa ccatctcaac aaactcttcc
     5581 agccctgttt cgcggaaatt caatgaagaa atcactgttt ccacataggt gagaaaagcg
     5641 ttattggtcc ggtatacctg cgaagcgaca aagcaataag gcaacaacgg caggtaatgc
     5701 tgctcaaaaa agcgtttact gatcccaacg ttcaatatac gcgtggcacc aaactcataa
     5761 aaactttgat ggtgcgaacc taacggaata aaaacaaaat cgccccgttc cagtaacacg
     5821 cgcttaccgt taatctcctg gaaataacgc ccggttaata ccagagtaaa ttcataatag
     5881 tcgtgctgat gcagcccgct gatactctca gttttgttat agataaacac atggaagttt
     5941 ttgccattaa acaactgctg ttctcgggca gtggcaattt ccggcgcgtt aatcactggc
     6001 tgcatcatcg actccttatg ccttcagttt ttcatgaagc tcaattaatt cagtaatcag
     6061 ttcacgcgca agcatggacg tcattaaatg atcctgagcg tggaccagca ccagactcac
     6121 tttcatctta ccttcgcccg catcgccttc aatcagtttc gtctgtacca gatgcgcttc
     6181 attcaatgcc atccgtgact gatccatcat ggcttttgct gcagcaaaat cgccctgctt
     6241 cgcctgtttc agtgccgcat acgccaggct gcgcgcttgt ccggagttga tgataagccc
     6301 catcaccact tcttccagct cttcagcttc cgtttgcgta tcgggaatgt tatcgagatc
     6361 catcatacat cgttcctctc tattcttacc ggcacgatta cccgtaccgg catcgattaa
     6421 aatttcaggg cattagcgat atcttcttcg ctctcttctt tatcaatcgc gttttgtgct
     6481 ttgttagcca ccacaacaaa gggcagataa attaacgttg cgataccaag gttgaagagt
     6541 gcgaccagca atgcggcgac gctaccgttg gtgttaaaga aggctcccag accggttggc
     6601 atggtccacg gtgcaatatt ggtcaccgga ggaatgatgc ccatgtagta cgctgcgagg
     6661 gtgattgccg ccagaatcgg ttgtaccagt acaaacggga taaacatcac cgggttcatg
     6721 ataattggca gaccaaacag aatcggttcg ttaatctgga agatgccgga cggcagcgcc
     6781 agttttgcca cctgacgata atcagcacgg cgagaggcga taaagatagc caggatcagg
     6841 cctaaagtcg caccactgcc cccaaggaaa ataaaggagt ccagcatcgg cttggcccag
     6901 atatggaagg tcttaccggc tgccagcgcc gcttcgacgg aaccatattg ctgataggtc
     6961 gcgatatttt ccagcgccca cggcgtcata atgccgttgt ccagtgcggt cagcgccagc
     7021 gcgccatgaa taccgaagaa ccagagcagt ggaacaaaga tcacataggc ccagcccacc
     7081 acgctaccca acgatgccag tggggttgag atggtatcca taatgatctg atggaagttg
     7141 gtgccccagg tattcaacgc ccaggcaata atccccatca cggaaagaat aataaagccg
     7201 ggaattaatg ccgagaagga acgcgatact gaagcaggta cgctgtcggg cagtttaatg
     7261 acccaattgc ggcggacaat aaaggtaaac atttctgcca ccaccaggcc aataataatc
     7321 ccggagatga tattcgcccc acctaaccag tttgcgccaa ccgcataggc ctcaccgaca
     7381 ctatatgggg tgacggtcat aaatgctgca acggataaca acccagccgc cagcgcatcg
     7441 actttacgct cttctgccag cgccatgcca ataaagaacg gtgccattaa agacattatc
     7501 cctaatgttc cgttatatac gttgccgcca atacctttca gaccattaag tgtttcaatg
     7561 gttgaggcgt cgaggcgaat acctaaggaa taaaaaaacg acccctcccc aaagcttaga
     7621 aaaacgttgt taattaatac aaacatggcc cccgcaaggg ttaacggcat taagcgaata
     7681 aagccatttt tgattgcatt aacgtgtggc tgctttccta ttttaactgc aaaagggagg
     7741 agtacctttt caagcgatgc aataacatta ctcatagaaa aataccctta aaaaccgcaa
     7801 tttaaatatt gcggta
//
LOCUS       DAZGVQ010000209         7683 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000209 DAZGVQ010000000
VERSION     DAZGVQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.211"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1034
                     /gene="cka"
                     /locus_tag="ACHLAG_004701"
     CDS             <1..1034
                     /gene="cka"
                     /locus_tag="ACHLAG_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="HFP0657800.1"
                     /translation="KKSNQLDADISKLNSYKNTLQSKIGDVNKQKEAEEKARENAEVA
                     EHETLNEEKQAVVEAEKRLAEAKAELAKAESDVQSKQATVSRVAGELENAQKSVDVKV
                     TGFPGWRDVQKKLQRQLEAKQAEYSAVENELKNAVSFRDGKAAEVKEAEQKLKEAQDA
                     LEKSQIKDAVDTMVGFYQYITEQYGEKYAKIAQDLAEKSKGKKIQGVDEALAAFEKYK
                     NVLDKKFSKVDRDAIFNALESVNYDELSKNLTKISKSLKITSRVSFLYDVGTDFKNAI
                     ETGNWRPLFVTLEKSAVDVGVAKIVALMFSFIVGVPLGFWGIAIVTGIVSSYIGDDEL
                     NKLNELLGI"
     gene            complement(1031..1321)
                     /gene="cki"
                     /locus_tag="ACHLAG_004702"
     CDS             complement(1031..1321)
                     /gene="cki"
                     /locus_tag="ACHLAG_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011264161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin K immunity protein"
                     /protein_id="HFP0657801.1"
                     /translation="MYLKYYLHNLPESLIPWILILIFNDNDNTPLLFVFISSIHVLLY
                     PYSKLTISRYIKENTKLKKEPWYLCKLSALFYLLMAIPVGLPSFIYYTLKRN"
     gene            1345..1476
                     /locus_tag="ACHLAG_004703"
     CDS             1345..1476
                     /locus_tag="ACHLAG_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072023513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFP0657802.1"
                     /translation="MKIVIFLITILFLSGCQANYIRDVQGGTVAPSSSSELTGIAVQ"
     gene            complement(1675..1818)
                     /locus_tag="ACHLAG_004704"
     CDS             complement(1675..1818)
                     /locus_tag="ACHLAG_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP0657803.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAAAIGNWIVDLDT
                     QNS"
     gene            complement(2483..2680)
                     /locus_tag="ACHLAG_004705"
     CDS             complement(2483..2680)
                     /locus_tag="ACHLAG_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFP0657804.1"
                     /translation="MNKQQQTALNMARFIKSQSLTLLEKLDALDADEQAAMCERLHEL
                     AEELQNSIQTHFEAQYGIEQP"
     gene            3301..3495
                     /locus_tag="ACHLAG_004706"
     CDS             3301..3495
                     /locus_tag="ACHLAG_004706"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFP0657805.1"
                     /translation="MNQDKAIYLENKYFSPANEAVRLEREGDYIGAWKKWNDALHYAK
                     GENIGWCIARAEYCKKTGRF"
     gene            complement(5726..6148)
                     /locus_tag="ACHLAG_004707"
     CDS             complement(5726..6148)
                     /locus_tag="ACHLAG_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Exc2 family lipoprotein"
                     /protein_id="HFP0657806.1"
                     /translation="MRSAAARLLLIPLITATIALTGCTPKTSLERHTRHYVYASDDGF
                     DPNFYTQKSDTIRMMLPFFQQFRDMGMKDKAAGVSAETAQQRVKEFHSEKFFHSLRST
                     TTFAGRKYTNSDMPSPKKMKLMADTISAVYLDGYEGRQ"
     gene            6270..6548
                     /locus_tag="ACHLAG_004708"
     CDS             6270..6548
                     /locus_tag="ACHLAG_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657807.1"
                     /translation="MKKCVPVLLFSVLSASVASHADSNVFQQKAEKTKNGIVADVNAR
                     QKMFSNRLKIQLQINELRAELAGTRDAQKREDLRKKLESLERQKKSLR"
     gene            complement(6540..6830)
                     /locus_tag="ACHLAG_004709"
     CDS             complement(6540..6830)
                     /locus_tag="ACHLAG_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657808.1"
                     /translation="MSKSPRHKLNKTDKRLLDTLVAAGYEYDKARDLIQKQVYTLTLA
                     DQRHVVSEISNGVNPTQAYSAVYQARRIRLARKYLNGKKVMEETGENTPPSA"
     gene            7071..>7683
                     /gene="cka"
                     /locus_tag="ACHLAG_004710"
     CDS             7071..>7683
                     /gene="cka"
                     /locus_tag="ACHLAG_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_159420795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pore-forming bacteriocin colicin K"
                     /protein_id="HFP0657809.1"
                     /translation="MAKELSGYGPTAGESMGGTGANLNQQGGNNNSNSGVHWGGGSGH
                     GNNGGQGNSNSSGSTSTVMKTGESYLTPWGDVVINNDGLPVMNGIVMTEENSTLVDNP
                     FGGVSRVLNSLISDMPSLFAESSGNNNNNTASVNTAPTNAQVSDMDKSSKVVGNVINE
                     KQKQKNKIATQISEKQKKIEEMKKVFKHHSYHGITDLERDVDEL"
BASE COUNT         2109 a         1582 c         1867 g         2125 t
ORIGIN      
        1 aaaaaaaatc aaatcagctt gatgcagata ttagcaaact taattcttat aaaaatacat
       61 tgcaaagtaa aattggtgat gtgaataaac agaaggaagc tgaagaaaag gctcgtgaaa
      121 atgcagaggt cgctgaacat gaaactctta atgaagagaa acaggcggtg gtggaagcag
      181 agaagcgttt agctgaggcg aaagctgaac tggcgaaggc tgaaagtgat gtacagagta
      241 agcaagctac tgtttccaga gttgcaggag agcttgaaaa cgctcagaag agtgttgacg
      301 tgaaggtcac cggatttccg ggatggcgtg acgttcagaa aaaactacag cgtcagcttg
      361 aagctaagca ggctgagtac tccgcagttg aaaatgaact taagaatgct gttagtttca
      421 gggatggtaa ggccgctgag gttaaagagg cagagcagaa attaaaagaa gctcaggatg
      481 ctcttgaaaa gagccagata aaagatgctg ttgatactat ggttggattt tatcaatata
      541 taaccgaaca atatggagaa aaatacgcaa aaattgctca ggatttagcg gaaaagtcta
      601 aaggaaagaa aattcaaggt gttgatgaag ctctggcagc atttgaaaag tataaaaatg
      661 tactggataa gaaattcagt aaggttgatc gggatgctat ttttaatgcg ttagagtctg
      721 ttaattatga tgagttatct aagaatttaa cgaagatatc aaagtcgctt aaaatcacta
      781 gtcgagtatc ttttctatat gatgttggta ctgattttaa aaatgcaata gaaactggta
      841 actggcgacc tttgtttgtc acgctggaga aaagtgcggt agatgtaggt gtggcaaaaa
      901 ttgtggcatt aatgtttagt tttattgttg gtgtgcctct tggtttctgg gggattgcaa
      961 ttgtcacagg tattgtttct tcttacatag gggatgatga gttgaacaaa cttaatgagt
     1021 tgttagggat ttaatttctc tttagagtgt aatatatgaa acttggcaat cctactggga
     1081 ttgccattaa taaataaaac aatgcagata acttgcataa gtaccagggc tcttttttta
     1141 actttgtatt ttctttgata tatctagata tggttaattt agagtatgga tatagcaata
     1201 catgtattga tgatataaag acaaataaca aaggagtgtt atcattgtcg ttaaatatta
     1261 aaataagaat ccatggtata agtgattcag gtaaattatg taggtagtat tttagataca
     1321 taatttttct tttgttggag gtgtatgaaa atagtaattt ttctaattac aatattattc
     1381 ttgtcaggat gtcaagcaaa ctatattcgt gatgtacaag gaggaaccgt tgcaccatct
     1441 tcgtcttctg aactgacggg gatcgcggtt cagtagaaaa gatcaaagga tcttcttgag
     1501 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctgccaacgg
     1561 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggctttagtg
     1621 gagcacagat accaaatgct gtccttccag tgtagccgct ataaggcttc aacttcaaga
     1681 gttctgcgta tctaaatcca ctatccagtt accaatggct gctgccagtg gcgttaagtc
     1741 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1801 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1861 ccaacagcgt gagctatgag aaagcgccac gtttcccgaa gggagaaagg cggacaggta
     1921 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccgg ggggaaacgc
     1981 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     2041 atgctcgtca ggggggcgga gcctatggaa aaacgccagt ggcgcggcct tttccgggtt
     2101 ctcgtttttt gcgcacatgt ttctttcggc gttatcccct gattctgtgg ataacggtat
     2161 caccgtcttt gagtgaggtg actccgctcg ccgcagccga acgaccgagc gtagcgagtc
     2221 agtgagcgag gaagcggaag tgtaccggaa gctgcatttt ctccttacgc atctatgcgg
     2281 catttcacac ccggcatggt gcacttttca tacaatccgc actgatgcca catgattaag
     2341 ccagtataca ctccgctacc gctacgtgac tgggtcatgg ctgcgccccg acgcccgcta
     2401 aagccccctg acgtgccctg acgggctttg tctgctggcg aaaacactca cggacaagcc
     2461 gcgaccactt tcgccagcgc ctttatggtt gttctatgcc gtactgagct tcaaagtgag
     2521 tctgtatgct gttctggagt tcttccgcca gttcgtgcag tcgttcgcac atggcggcct
     2581 gttcgtctgc atccagtgca tccagttttt cgagcaacgt caggctctgg cttttgataa
     2641 atcgcgccat gttgagcgcg gtctgctgct gtttgttcac gttctcccgt ctcctttact
     2701 ggtgtttacg tccggcagtg cgtcacctgg tgtgatgacc cggtgacgtc actgaaaatt
     2761 atcacacaag cccagtcgcg cgtgtgctgc gcgacattaa caccccgtga cgcttatttt
     2821 ttgctgaata tgtgcgcgat tttctgtctg cgttgtgtgc agttgttgtc ataaacacga
     2881 atgttccggc tgttcctgat gtgcgtcact ttgacgtttt gacaaagtga cgcacgggag
     2941 gtgcttgcgc aaacccgcga ataacggcgt ttaccgcgtg attttttaac atctgacaaa
     3001 gtgacccggc gtgatggcca gttctggcca gagacgttgg cggggtgtcg gggcgcagcc
     3061 atgactaggc ggcatttgtc tgattgcgtg tgcgcggtcc gacaaataca catcctgtcc
     3121 cgtttttcgc acggttttta ttgattaccg gacagatgaa cccggattgc catgttgcga
     3181 aaatcacgtt aaaacgatta cgagcgtgct gttgggctga tatatcaaca gtcatgccaa
     3241 tatgtattaa cccgatgggc tcgctctttt ttaattcaaa taaaaatata aattacgaat
     3301 atgaatcagg acaaagcaat ttatttagaa aataaatatt tttcacccgc aaacgaagct
     3361 gtcagactgg agcgtgaagg tgattacatt ggagcatgga aaaaatggaa tgatgcgctt
     3421 cattatgcca aaggagaaaa tataggttgg tgtatagcca gagctgaata ctgtaaaaag
     3481 acaggccgct tttaattctg gcggggtttc aaaggggcgt ccactttggc gcgtcgacgt
     3541 atcaaaatgc gccagcattt tgtatacagt cggtcacgtg cctccagaat ttaccctcaa
     3601 aacgcccctg caactgatac acaacaaaag tataactaat atgtagttat tatataacta
     3661 atgcataata aacagctcac caacggatcg catgtagtag acgctacata aaaaatcctg
     3721 aaatggagga tttgtcataa agagattcct gtttttttag cgttctgatg caggacaggg
     3781 caaacaccgc aaaaatgatg aaaaactgat taaccatatt gtctgtattc tgaatgaatt
     3841 acgaagcata gcgataaatt aaaaaatccc cctggattac agggggaagc catcagagtt
     3901 tgagtaatga gaaatattat tttggagaga gcaccacaaa tgcgctcttt tccaatctgc
     3961 cgtaaccatc ggcaaaagac aatcaggaca ggcgaaaaaa tccccctata tggcaagggg
     4021 gtgtcaatga ggacatggca attgtgagtt ttgtgtgtgt tttatgtgtt gcagtttgca
     4081 acgtcagaaa tttgccgtag ttcgtcatga gagacaacaa aaaaatcccc cgtgactggt
     4141 ggaggccagg gcaagggggg tagcaaatgc ctgaataatt ttcgtagttt tgatataccc
     4201 atcgttgcaa ccgcaacggg atgaatttgc cgcagttgtg cgcgaaaaac aatcctgatt
     4261 ccgggacagg atgtgtaatc gtataaccgc gcacgcacgg ctatgcgatt accacctggt
     4321 cagggctttg ccccgacagc ctcgacaccg ttcccgcgat acgatagaac atcagggcgc
     4381 gtttccgggt aaacgcatgt taatgaccgg gaattcattg atcagggacc ggagacgcgc
     4441 tacaaaactc ctgcgcgttt ctggcatcaa ctggccatgg ggatggtttg gcagtagcag
     4501 caagcgccag aaacgcgctc aaaacgtctc taacagcctg aacccaccaa aacataatga
     4561 aacattgcca gatgcaaaaa agtgcgttag aaagcgtctg agcgcgtttc cttgccacct
     4621 gaacgtcatc tctgggattc gagctcactc catcaaataa caaataaact tcaaagtctg
     4681 ataatgggtt ccccctgtaa tccaggggat ttttttcgct cactcattac cgcacaactc
     4741 gacatcacgc cggagacacg ctaaaacacc ctgcgcgttt ctggtgcaaa gagtagttcg
     4801 tagggatgat tacagtcagg tatgtaccag aatcgcgccc tgaacacggc tgaacgcctc
     4861 aaagcgaaac cattgcctca tgtaaaaaat cgcgttacaa agcctctgag cgcgtttctg
     4921 gcgtcttttt tgttgctgtg tcgctatact cgttgtcaga ttcgcgctca atcatcactg
     4981 attaatctgc gcttatcctt cgaccgatca cgctggtatt ttttgacaac cggcgcccat
     5041 gaaaacgcca gacgggaacc gtcatccgca ccctctgaca tgctggggat taaaaatccc
     5101 tgtgccgcac agccggcgtt gcataaaatt ccgctctgcg atgagtattg tttaatcaaa
     5161 aatccccgca gtttctccag aaaaacgcga gtctgttctc atgccccgga tgcccacccc
     5221 ccacgcaaaa ccaacttttt tctgatttgc tttttaaatc atgttgttat ggtttgaaaa
     5281 atatattttc ttattcagat ttatacaaaa gcgagttgtc ggcgactact ggatgattcg
     5341 gcaacgagtc gtcggtgatt tatcgatgac tcaacacact gtctctctgg tgttcagaaa
     5401 aagcgagtcg ccagagagtc gcgaggaaaa atactggatg actcgctgat gactcccggg
     5461 gtgaatcgct cacgactcgt ctgtaactac tgtctgattt cttctcatca gggctgaaaa
     5521 cgtccgaaat gcgccttatg ggcatcaggc gcgcccaaac cgatcgccat tcagtttcat
     5581 gcatagtcat gcagtgccat gaaaacaatc ctgacgtatt tttccggttt acccggggga
     5641 aaacatctct ttttgcggtt tttgcgccag aatcgcgctg agcgcgtttc agcggtgcgt
     5701 acaataaggg attatggtaa atcccttact gtctgccctc gtatccatcg agataaaccg
     5761 cagaaatggt gtctgccatt agtttcattt ttttcggcga aggcatatcg ctgtttgtgt
     5821 attttctgcc agcaaaggtt gttgtgctcc ggagtgagtg aaaaaatttt tctgagtgga
     5881 attcttttac acgttgctgt gccgtttctg ctgatactcc ggctgctttg tctttcatcc
     5941 ccatatcccg gaactgctga aagaaaggga gcatcatacg tatggtgtct gatttctggg
     6001 tgtagaagtt aggatcaaat ccatcatctg aagcataaac ataatgccgg gtatgtcgtt
     6061 ccaggctggt ctttggtgtg catcctgtaa gagctattgt tgctgttatc agaggtatca
     6121 gaagcagcct ggcagcggca gatctcataa acatctcctt gcgtttttaa tcttgatact
     6181 cagggagtta tatctccgaa gagtatctct tatcatacgt cggtttactc ccaatgtgat
     6241 ttgatggtca atgagtcagg aataatcccg tgaaaaaatg tgtgccagtc ctgcttttta
     6301 gtgttctttc tgcttcggtt gccagtcatg cggacagcaa tgtttttcaa caaaaggccg
     6361 agaagacaaa aaacggcatt gttgcggatg ttaatgccag acagaaaatg ttcagtaaca
     6421 gactgaagat acaacttcag atcaatgagt tgagagcaga gctggctggc accagagatg
     6481 cccaaaaaag agaggatttg cgtaaaaaac tggagtctct ggagcggcaa aagaaatcct
     6541 tacgctgatg ggggcgtatt ttccccggtt tcttccataa cctttttacc gttcagatat
     6601 ttacgggcga ggcgaatgcg tcttgcctgg tataccgctg agtaagcctg ggtgggattc
     6661 acaccattac tgatttcact gaccacatga cgctgatcag ccagtgtcag cgtgtaaacc
     6721 tgtttctgga tgaggtcacg ggctttgtca tactcatatc cggcagcaac aagggtgtcg
     6781 agaagtcgtt tgtctgtttt gttcagttta tggcggggtg atttgctcac atcgttctcc
     6841 gttttttcat gatccaacag ttttatatct catcctgatt tgctccggtg ttctgtttcg
     6901 acgttctcaa tgcctgcctc cccatagtgt gatccttatt gcaaaaatga aaatccatgc
     6961 tcttgacacg gacaatgctg agtagtaggt tttcactgta cataaaacca gtggttatat
     7021 atacagtata ttgtttttaa tttattgttt taattatcaa agaggaattt atggctaaag
     7081 aactaagtgg atatggccca actgctggtg agtctatggg gggaacaggg gcgaatctga
     7141 atcagcaagg tggaaataat aatagtaata gtggggttca ctggggtggt ggctctgggc
     7201 atggtaataa tggtggtcag ggaaattcaa attcatctgg ttctacatca actgtgatga
     7261 aaaccggtga gtcatatctg actccgtggg gtgatgttgt cattaataat gatggccttc
     7321 cggtgatgaa tggtattgtt atgacagaag agaattcaac tctggttgat aatccatttg
     7381 gaggggtctc cagagttctt aattctttaa ttagtgatat gccatcactt tttgcagaaa
     7441 gctcaggaaa taacaacaat aacactgctt cagttaatac tgcaccaacg aatgctcagg
     7501 tcagtgatat ggataagagt tcgaaggtgg tgggtaatgt aattaatgaa aaacaaaagc
     7561 agaagaataa aattgctaca cagattagtg aaaaacagaa gaaaatcgag gagatgaaaa
     7621 aagtattcaa gcatcattct tatcatggta tcactgatct tgagagggat gttgatgagt
     7681 tgc
//
LOCUS       DAZGVQ010000210         7669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000210 DAZGVQ010000000
VERSION     DAZGVQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.212"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            47..265
                     /gene="yiiE"
                     /locus_tag="ACHLAG_004711"
     CDS             47..265
                     /gene="yiiE"
                     /locus_tag="ACHLAG_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP0657810.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKRGRQRGETVGVYDQCKDHKE"
     gene            485..727
                     /gene="yiiF"
                     /locus_tag="ACHLAG_004712"
     CDS             485..727
                     /gene="yiiF"
                     /locus_tag="ACHLAG_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFP0657811.1"
                     /translation="MNSLVGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1057..1986)
                     /gene="fdhE"
                     /locus_tag="ACHLAG_004713"
     CDS             complement(1057..1986)
                     /gene="fdhE"
                     /locus_tag="ACHLAG_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFP0657812.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1983..2618)
                     /gene="fdoI"
                     /locus_tag="ACHLAG_004714"
     CDS             complement(1983..2618)
                     /gene="fdoI"
                     /locus_tag="ACHLAG_004714"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP0657813.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2615..3517)
                     /gene="fdoH"
                     /locus_tag="ACHLAG_004715"
     CDS             complement(2615..3517)
                     /gene="fdoH"
                     /locus_tag="ACHLAG_004715"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFP0657814.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3530..6580)
                     /gene="fdnG"
                     /locus_tag="ACHLAG_004716"
     CDS             complement(3530..6580)
                     /gene="fdnG"
                     /locus_tag="ACHLAG_004716"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5993..5995),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP0657815.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTC
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6774..7607
                     /gene="fdhD"
                     /locus_tag="ACHLAG_004717"
     CDS             6774..7607
                     /gene="fdhD"
                     /locus_tag="ACHLAG_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFP0657816.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1769 a         1980 c         2044 g         1876 t
ORIGIN      
        1 aattttcgca catacattaa tgagtaatat atgtaaggtg gtggtaatgg cgatgaatac
       61 ggtttttctt catttatcag aagaggcaat taaacggctg aacaagcttc ggggctggcg
      121 taaggtttca cgatctgcaa ttttacgcga agcggttgag caatatctgg agcggcagca
      181 atttccggtg cgtaaagcaa agagaggcag gcaaaggggt gagacagtag gtgtttatga
      241 tcagtgtaag gatcataagg aatgacaaaa atttttttat tcttgaatat aaaaaaccag
      301 atgcccttat tctggtatta atacaaggtt gttttacttg aacttataat aactgcaact
      361 gttacatcat atctggaaaa cgcctcgcaa aaataaaaaa tgattcgtaa atgagctttc
      421 tttaaagatt cgacaagggt acttttctct ccatagattc aaaaatgata ttggcgagat
      481 atttatgaac tctctggtag gtatagatat gggcagaatt ttactcgatt tatcgaatga
      541 ggtgattaag caacttgatg atctcgaggt gcagcgtaat cttcctcgtg cggacctatt
      601 aagggaagcg gtagatcaat acctgataaa tcaatcgcaa acagcaagaa ccagtgttcc
      661 tggcatctgg caagggtgtg aggaagatgg tgtcgaatat cagcgtaagc tgcgcgagga
      721 atggtaatgg gaaaaaatga tgtcaatcag attgctgaca acgtgcgcgt tgttcatgcc
      781 ggatgcggcg tgaacgcctt atccggccta caaagtcatg caaattcaat atattgaagt
      841 ttattggtag ggctgataag cgcagcgcat caggcaatca tacgcttgtc atcagtgaca
      901 tacccataac cttatcgatg tgttggaggg aagaacgagg caaacagaca attgcggcat
      961 gatcttgaat gggtaacgcg gaatacgaga gatggtgtcg tcacacccta tgagctgtaa
     1021 tccgggcgac cagcgccacc cggaaaacgt cagagattac tccccttcac ccggaaacag
     1081 gaacgggttg atggaactgc gggcatagcc ttcttgctcc attcgcgcgt ccagtaccag
     1141 agaagcgagg tcatctgcca cggcttcaac tttcggttct ttttcctgat aaagaatctt
     1201 caggtaagtg ccacagtcat cgcagctttc ggctttaatc gcggcctgtt cgtcatccag
     1261 cgaccagtaa tgcagtttgc cgctctgctc gcagttgctg cattttacgc gcactacgtg
     1321 ccattcggtt tcacacaggt tgcagtgcag gtaacgcaga ccctgagtgg tgccaatttg
     1381 caccatgctg gacaccggca tagaaccaca aaccgggcaa tattgacgtt gctcgccgta
     1441 ttcagcgcgg gctttgccgg ggatcagatt ggccatctgc gcccagtaga gcgacagtgc
     1501 agcccagata aacggcgctt tatcgctgct gacggatgag aaatcagagg caaacagtgc
     1561 gctggccata tcttccagct cctgagttga tgccttctcc agattctcaa tcactgccag
     1621 cgccgggccg ctcatttcag gtttcagctc agcaatcagc gccatcagca gcttttgcca
     1681 gtgcttatca cgcggcagaa cgtgaatatc cagcgggggc ttgccctgtg cgctggcttc
     1741 tttaatgcgc gcggtcagat ccatctccag cggatggtcg tacagcacca cttcctgggc
     1801 gtgggcgata agcgcagcaa agcgcaggta atcacccagc ggattatttt ctgccagctc
     1861 gcgcagacgc tcggcgcggc ggttgtataa attcttgagc cgagggaaca ataacggcgg
     1921 aatcatatcc gccgtacgtt tctcgctcga acccagctca tcttgcggga ttatgcgaat
     1981 actcattcag ctttcttttc cgttgtcttg cggacctcac ggtaccagcg cgggtgatgt
     2041 ttcttcgccc aggcgctggt aacccatcct tccaccatgg cggtaatcgt gcctttcacc
     2101 caaagggcgg cgtagatatg caccatgata accacaatta acgccactgc ggcaaatgaa
     2161 tgcagcatta acgcgaatcg gatcaccggg attgagaaag caggcgcaaa ataaggacgc
     2221 cagataatca cgccgctcac cagcagcaga accaggaaaa taatcgccgc ccagaaaacg
     2281 catttctgac cgaagttata acgcccggtg tcacctactt cctcgttgac gacgatctta
     2341 cgaatattct tcgcccaaaa gatatcatcc cgattgatta ggttgtgatg ccagtaacgg
     2401 aaaaacatga tgatgaacga ggcaaacata accacgccga caaacgggtg cagaattcgc
     2461 gccagctgcg gtgtgcccat gatttgcatc aaccagttga aggacgggaa caaaaagccc
     2521 agcccgctca ccgccgccag gatgaagcag aaggcggtga tccagtggtt gatacgttcc
     2581 ggcgcggtgt agcgcacgat ggtgtcacgt cgtttcattt gcgctcctcg tctttctctt
     2641 cgtgcagatt attctcttcc tcatccgcac ggttcggacc gacacccacg tagtggaaga
     2701 tactggccgc aaaggtagcc gcaaagccaa cagccgcgag cggtttccag atgcctttcc
     2761 agaatttcac ggtttcgctg atttccgggt tctccggcaa gccatgatac agatttggct
     2821 tgtcagcatg gtgcagcacg tacatgacgt gtgtaccacc gacgcctgcc ggatcgtaca
     2881 gacccgcatt gtcgtaaccg cgggttttca gctcagcaac gcgctcgctc gccagcgttt
     2941 tcatcgactc tttcgtaccg aagtgaatcg cgcccgttgg gcaggtcttc acgcaggctg
     3001 gttcttgccc aaccaccacg cggtcaacgc acagcgtaca tttgtagacg cggttgtctt
     3061 ccgggttgag gcgcggaatg tcgaacggac agcccgcaat gcaataaccg cagccaatgc
     3121 actgctcgga ctggaagtcg acgataccgt tggcatactg aataattgca ccttccgccg
     3181 ggcacgcttt caggcagcct ggatcggaac agtgcataca gccgtctttg cggatcagcc
     3241 attccagttt gtcgttctgc tccacttccg agaagcgcat taccgtccac gatttggcgc
     3301 ttaaatcatt ggggttgtcg tacaccccaa tgttattgcc gacggtatcg cgaatgtcgt
     3361 tccactctga acacgccacc tgacaggctt tacagccgat acaggtggtg acgtcgatga
     3421 gtttcgccac ttcttcctgg aagtcccgcg cctgaggcgc gggggtcaga ccgttagtcg
     3481 cggaacgacg aatgatatct tgagattgat aagccatatg tcgtctccgt tacacctttt
     3541 ccacattcac aaggaaggac ttaaactccg gcgtctgcgt gttcgcatca ccaacgaatg
     3601 gcgttaacgt attggcaata aagccttttt tagctacgcc ttcatagccc cagtgaatcg
     3661 gaataccgat ggtatcgata tctttgccgt tcgctttcag tgtacgaata cgtttggtca
     3721 ccaccgcttt ggccttgata tagccacggt tggaggagac tttcacggta tcgccctggg
     3781 caatgccaag tttattcgcc agcgactccc cgatttccac aaactgctct ggttgcaaaa
     3841 tcgcgttcaa cagcgcgtgt ttggtccagt agtggaaatg ctcggtcaga cgataggtgg
     3901 ttccgacata cgggaactta tcggctttac ccaatgcttc ggcgtcgtct ttaaagatac
     3961 gtgcagccgg gttcgagata acgtttggat gcagcgggtt agttcccagc ggcgtttcaa
     4021 acggctcgta gtgttccggg aacggacctt ccgccatctt atcgagggca aacagacgcc
     4081 ccatgccttc ctgctgcatg ataaacggcc cgacgccgct acccggaggc gctgcgctgt
     4141 agtccggaat atcccagccg gtccacttag tgccgtccca tttcagcaac tgacgcttcg
     4201 gatcccacgg gttaccctgc ggatctgcgg aggcgcggtt atacagaatg cggcggttaa
     4261 gcggccatgc ccatgcccag cccagcgtgt taccgaggcc agacggatcg gcgttatcac
     4321 gacgcgccat ctggttgcct tccggcgtcc agctaccggc gaaaatccag cagccacagg
     4381 aggttgtacc gtcatcgcgc aattgggcga acgagctaag ttgttggcct ttcttgacga
     4441 taaccgcacc ggttgccgga tcggtaatat cggccagcgc cttaccgttg ctctccatcg
     4501 ctacttcttc cgacttcggc tcatgcggaa tggcgtagtt ccaggtcatg ttcagcacct
     4561 gatccgggtt cgcgccaccc tgttcggcat acatcttgcg caagcgcagg aagataccgg
     4621 agaggatctc accgtcagtc agcgcaatcc ccggggcgtc cgcacctttc cagtgccact
     4681 gcaaccagcg acctgagtta acgattgaac cgttctcttc tgcgaagcag gtcgatggca
     4741 gacggaacac ttcggtctgg atcttcgacg agtcaacttc gttcagctca ccgtggttct
     4801 gccagaagtt agaggtttca gtgttcagcg ggtcgatggt gacgaggaac ttcagtttcg
     4861 acagacaacc gatcactttg tttttgttcg ggaatgaggc aacagggtta aagccctggc
     4921 agatatagcc attgaccttg ccttctttca tcatctcgaa gtattgcagg acgtcgtagc
     4981 ctttatccca cttcggcaac cagtcaaagc cccagctatt ttccgccgtc gctttatcac
     5041 caaagaaggc cttcatcata gagacgaaga atttcgggta gttgccccag tagtttacct
     5101 ggccttccag cagcggtttt ggcgtgttgg cggtaaggta ggtttgcaga tcggtctgct
     5161 tctcgcttgg cagcgtcatg taacctggca gactctgcga cagtagcccc aggtccgtca
     5221 gcccctgaat attggagtga ccgcgcaggg cgttaacgcc gccgccggcc atccccatat
     5281 tgccgagcag cagctggatc atcgccatcg tacgaatgtt ttgcgcacca acggagtgtt
     5341 gcgtccagcc gagggcatac aggaacgagg cagttttatc gtgagcactg gtttctgcga
     5401 tgtattcgca gactttcagg aacgcgtctt ttggcgtacc acagatgttt tcaaccacat
     5461 ctggcgtgta gcgggaaacg tgctgtttca gcaagttcca cacgcagcgc ggatgttgca
     5521 gcgtggtatc gcgtttggcg aagccgtttt cgtccagttc ataagtccag gaggatttat
     5581 cgtacttgcg tttttccgcg tcgtagccgg tgaacaggcc atcttcaaag ccgtaatcct
     5641 cacggactat caggctggcg ttggtatagg cttcggtgta ttcgcggttg aatttttcat
     5701 tgttcagcag gtacagcaat acgcctgaca ggaaagcaat gtcagtaccg gaacgaatag
     5761 gggcatagta gtcagctacc gccgccgtac gcgtaaagcg aggatcgatc acaatcagct
     5821 tcgcgccgtt gtgaattttg gcttccatcg cccagcggaa cccgaccggg tgagcttcag
     5881 cggcgttacc gcccatcacc acgacgaggt tggcgttctt gatgtcgacc cagtggttgg
     5941 tcatcgcacc gcgaccaaat gttggagcaa gacttgctac cgttggtccg tgtcagacac
     6001 gcgcctggtt gtcgaccgcg agcataccca gcgcgcggga gaatttttgc gttaaatagc
     6061 cggtttcgtt actcgacgcg gaagcacaca gcatcccggt ggagagccag cggttaacag
     6121 tcacgccttc ggcgttttgc gcaatgtagt tagcatcgcg gtcttctttc atcagtttgg
     6181 cgatgcgatc aaacgcctct tcccaactga tttgctgcca tttatcagaa cctggcgcac
     6241 ggtattccgg aaacttcaga cggctttcgg agtggatgaa atccaccagg ccagcgcctt
     6301 tcggacaaag tgcaccgcgg ttgaccgggt gatccggatc gccttcgata tggaagatag
     6361 atgctttggc gttttttgct ccgtcaccga ggctgtacat caacagccca cagcctacgg
     6421 aacaataggt gcaggtatta cgggtttcgc aggtgcgcag cagtttatac tgccgggttt
     6481 ccgcgagtgc tacgctgggt gcaaaaccca gtgccgctgc cgtggtgcct gccataccgc
     6541 cagcgcagat cttaaagaac tgccttctgc tgacctgcat ggattgctcc ttgtttcgac
     6601 attgtcacgt cccatttaca ttcgcttgct gcatgtgcag ggagtgggag ttatttttct
     6661 ttgcggaagg ggccgcaaag gtccagaatt ggctcaattt ccctccatcc aagaagggtt
     6721 tgtaacagaa taccataatg ttggtgtgtg tgttcttatc tggttaagag aaagtgaaaa
     6781 aaacacagca aaaagaaatc gaaaatgtga caaatatcac aggtgttcgt caaattgagt
     6841 tatggcggcg tgatgattta caacacccac ggttagatga ggtcgcggaa gaagttcccg
     6901 ttgcgctggt ctacaacggc atttcgcatg tggtgatgat ggcgtcgccc aaagaccttg
     6961 agtactttgc gctcggtttt tcgctttccg aggggataat agaaagtccg cgcgatatct
     7021 tcggcatgga tgtcgttcct tcctgtaatg gtcttgaagt acaaattgag ctttccagcc
     7081 gccgctttat ggggttgaag gagcgccgcc gggcgctggc gggacgtacg ggctgtggcg
     7141 tatgcggcgt ggagcaactt aatgacatcg gaaaaccggt gcagccgcta ccgttcaccc
     7201 agacgtttga tctcaacaaa ctggatgatg cattacgtca tctcaacgat tttcagccag
     7261 tggggcaact gactggttgt actcacgccg ctgcctggat gttgccatct ggcgaactgg
     7321 tcggcgggca tgaagacgtg ggtcgccatg tggcgctgga caaactgtta ggccgccggt
     7381 cacaagaagg ggaaagctgg cagcaaggtg cggtactggt ttccagtcgt gccagttatg
     7441 aaatggtgca aaagtcggcg atgtgcggcg tagagatttt gtttgcggtg tctgccgcga
     7501 ccacgcttgc tgtagaagtg gctgagcgct gtaatctgac actggtaggt ttttgtaaac
     7561 cgggtcgggc aacggtttat acccatccgc agcgtttgag caattaatta aaataagtcc
     7621 tgcgatatat attaaattgc aggacttatt catttcgtga attttatta
//
LOCUS       DAZGVQ010000211         7603 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000211 DAZGVQ010000000
VERSION     DAZGVQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.213"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(128..832)
                     /locus_tag="ACHLAG_004718"
     CDS             complement(128..832)
                     /locus_tag="ACHLAG_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP0657817.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(969..1421)
                     /gene="moaE"
                     /locus_tag="ACHLAG_004719"
     CDS             complement(969..1421)
                     /gene="moaE"
                     /locus_tag="ACHLAG_004719"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFP0657818.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(1423..1668)
                     /gene="moaD"
                     /locus_tag="ACHLAG_004720"
     CDS             complement(1423..1668)
                     /gene="moaD"
                     /locus_tag="ACHLAG_004720"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFP0657819.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHSLTDGDEVAFFPPVTGG"
     gene            complement(1661..2146)
                     /gene="moaC"
                     /locus_tag="ACHLAG_004721"
     CDS             complement(1661..2146)
                     /gene="moaC"
                     /locus_tag="ACHLAG_004721"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFP0657820.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2149..2661)
                     /gene="moaB"
                     /locus_tag="ACHLAG_004722"
     CDS             complement(2149..2661)
                     /gene="moaB"
                     /locus_tag="ACHLAG_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFP0657821.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREIEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(2683..3672)
                     /gene="moaA"
                     /locus_tag="ACHLAG_004723"
     CDS             complement(2683..3672)
                     /gene="moaA"
                     /locus_tag="ACHLAG_004723"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFP0657822.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(3660..3804)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4069..4977
                     /gene="yvcK"
                     /locus_tag="ACHLAG_004724"
     CDS             4069..4977
                     /gene="yvcK"
                     /locus_tag="ACHLAG_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFP0657823.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIMIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5169..7190)
                     /gene="uvrB"
                     /locus_tag="ACHLAG_004725"
     CDS             complement(5169..7190)
                     /gene="uvrB"
                     /locus_tag="ACHLAG_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP0657824.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
BASE COUNT         1830 a         2018 c         1902 g         1853 t
ORIGIN      
        1 atttatggat acggaaaacg cttccgctcg ccataaggcg tattttatac ctggtacttg
       61 ttaagtgtcg tttttagtaa gaaaaaaaat cccgacacat ggccgggata ttgatggtga
      121 aaaagaatta acggcggttg ccaaaaattc gcagcaacat caggaacagg ttgatgaagt
      181 ccagatacaa ggttaacgcg ccaagaatgg aatatttgcg caggttcgac gtgtcgcggg
      241 tatcaatctg ctcacccata tttttcaatt tctgagtgtc atacgccgtc aatccgacaa
      301 agacaatcac gccgatgtag gtaactgccc acatcaatgc ttcgcttttc agccagaagt
      361 tgaccagcga tgccagcaca atgccgatta acgccataaa cagcatattg ccaaagccac
      421 ttaaatcgcg cttcgtggtg taaccgtaca ggctcattgc gccgaacatc ccggcagtaa
      481 cgacgaaagt actggcgata gaagcagcgg tatagacaat gaatatactg gaaagcgtaa
      541 gacccgtcag cgccgaataa agcataaaga gcatcgtcgt tacacctgcg ctcagctttt
      601 gaatcatcgc tgataacaca ataaccaatg ctaattgcgc gatgatcaga ccgattaaaa
      661 agacacggtt agtaaacaac agctccatca cggccgcgga attagccgca taccaggcaa
      721 caaatgcggt cagcaacaag ccaacggtca tccagccata gacttgcgcc atataagttt
      781 gcaagccagc ccggggttgt acgattgaat cagaacgtgg gaatctgtcc atgacgatct
      841 cctgaagata taaggaatat cttaaggata ctgcaaaatg atgaggctgt gcatcgacgc
      901 agcgtaaacg catgtactga gcggtgaaat tgccggacgc agcggtgcct tatccggcta
      961 acaaaaaact accagcgttt tgccgcctgc tgatcgctct cccgggcttc aacccagcgg
     1021 tcgccttccg gcgtggcttc gcgcttccag aacggtgcgc gggttttgag ataatccata
     1081 ataaactgcc cggcttcaaa cgcactgctg cgatgcgcac tggtgacacc gacaaaaacg
     1141 atttcatcgc ccggccataa ttccccgatg cggtgaatca ccgtgacgcg ccccagcggc
     1201 cagcggttac gcgcttcatc aacaatttct gccagtgctt tttcagtcat ccccggatag
     1261 tgttcgaggg ttaatgcttt gacgctgtcg cccaggttat ggttgcgcac cttaccggta
     1321 aaggtgacta ccgcaccgtc ttcgtcacgc tccgccagcc acgggtactc ttctcctacg
     1381 ctgaacggct gcgggccgac aacaattttg gtttctgcca tcttaacctc cggttaccgg
     1441 cgggaagaaa gctacttcgt cgccgtcagt cagcgaatgg tcaaaactca ccagcgtctg
     1501 gttaacggca gccagcaatt tgccatcctc cagcgccaac gcccagcgat cgctctgcgc
     1561 agccaggtgc tggcgtaacg cttcaacggt tgggaaatcc gcagccactt cggttgcatc
     1621 tgttcccacc aactcgcgca cctgggcgaa aaaaagaact ttaatcatcc gcttccacct
     1681 taaagtcacc cgacttgccg ccgctcttcg ccagcaaacg taccggacca atcaccatat
     1741 ctttttgcac cgctttgcac atgtcataaa tggtcagcgc cgccacggag gccgcggtta
     1801 atgcttccat ttcgacaccg gttttcccgg tcaggcggca taaggtttct atacgcaccc
     1861 gattgtgctc cggctcggcc tgtaaattga cttcaacttt gctgagcatc agcggatgac
     1921 agagcgggat cagatcccag gtgcgttttg ccgcctgaat accggcaata cgcgcagtgg
     1981 caaatacgtc gcctttgtgg tggcgaccat caataatcat cgccagcgtc tcgctgcgca
     2041 tggtgacaaa ggcttccgcc cgcgcttcac gcacggtttc cgctttggcg gagacatcca
     2101 ccatgtgcgc ttcgccagcg gcgttgatat gggtcagttg cgacatactt atttcttcaa
     2161 atgtggatgg aaattacacg gacgcgtacg ggcatccagc tgcggcgcga tgatattttc
     2221 ccatgcggta cggcacgctt tggtcgaacc cggcatggcg aaaatcagcg ttttgttggc
     2281 gacgcccgct accgcacgag attgcaacgt ggaagtgcca atctcttcaa acgacaacat
     2341 acggaacact tcaccaaaac cttcaatttc acggtcgaac aacggcagca atgcttcggg
     2401 agcctgatca ccttccgtca ggccagtacc acccgtaatc aataccactt gtacatcgtc
     2461 gctggcgatc cacgcagata cctgagcgcg aatagcgtag cggttttctt tcacaatggc
     2521 tttatcgaca acgtgatggc ccgcttcttg cgccgaatcg cgcagatagt gaccggaggt
     2581 atcgtcttct tcaccgcgac gattagaaac cgtaagaata gcaatacggg tcgggataaa
     2641 ttcagtgctt acctgactca tctgatctct ccttttgacg ttttagccgc caatgtacga
     2701 taagttttgc gtaataccgg tgttgttttg atgcaggaaa tgggtctgtt tcttctcccg
     2761 cagcgccgct gaaatacgcg cttccagcgc ctgttgctgg gtatcgtctt ccagcagatc
     2821 gcgcaggtta acgccgcctt caccaaacag gcagagatgg agtttaccaa tggaggaaac
     2881 gcgcaggcgg ttgcaagtgg cgcagaagtc tttttcatac ggcatgataa ggccaatctc
     2941 tccggcgtaa tctggatggc aaaagacttg cgcgggaccg tcgctgcgtt gacgtaattg
     3001 gtggatccag ccgcgacgca gtagctcgtc acgcagaacc tgaccagaga tgtgatgctt
     3061 acggaagagc tcgctgccct cgcccgtttc catcagttcg atgaaacgca gctggatagg
     3121 gcgatgctgg atccagttca gaaaggtgtc gagctggtga tgattaacat cacgcatcag
     3181 cacggtattg actttgacct tctcaaaacc ggcctcaaat gcagcatcaa tccccgccat
     3241 gacctggttg aatttatcct gcccggtaat agcgtgaaac tggcgggcgt ccagactgtc
     3301 gacactgacg ttaatgccag taagtcccgc atcgcgccag ttcgccacat cgcgttccag
     3361 acggtaacca ttggtggtga ccgcaatctg gcggatagcg tcgttttccc gcacagcggc
     3421 gatgatatcg gtaaagtcgc ggcgtaaaga cggctcaccg cctgtcaggc gcactttttc
     3481 ggtgcccaga ctggcgaagg cgcgcgtaac ccggcgaatt tcatcgacgg taagaaagcc
     3541 tttattggtg acgccgctcg gtttgtagcc atccggcagg cagtaggtgc aacgaaagtt
     3601 acacacatcg gtaatcgaca ggcgcaagta gtaaaactta cgcgcaaatg catcagtcag
     3661 ttgtgaagcc atgtacacct ttccagatac gggaggcgaa gtcatttctt ccttcgccct
     3721 ggtggcaatc tttacgagca ttgtcacggc caaagcaccg tatcagttga cccaggtgca
     3781 gaggctagag tgtttagtgg ttatgccgat actagcgtgt aaatgagatt tttaccatcc
     3841 acactttcgc tatgtaatca tgtatatagc gtcatgatcg tgctatttcg ctacagaata
     3901 agggaaaatg gcggttttac tttgatatac atcatcaggg aggcatagcg tgcatcgtcc
     3961 tttagaggta gaagaaaata gtttgtatcc tcaatattgg caggttaatt gctgtttccc
     4021 cgcaatttgc gctactgtag cgcggataat ctgactccag gaaactatat gcgcaatcgt
     4081 acgctggctg atcttgatcg tgtcgttgct ctcggcggag ggcatggact gggacgcgtt
     4141 ctctcatcac tttcgtcttt gggttctcgt ttaacgggta tcgtcaccac caccgataat
     4201 ggtggctcga cggggcgtat tcgccgttca gaaggcggca ttgcctgggg cgatatgcgc
     4261 aactgcctca accagctgat aacggaaccg agcgtcgcct ccgcgatgtt tgaataccgt
     4321 tttggtggca atggcgaact ttccggtcat aatctcggaa acttgatgtt aaaggcgctg
     4381 gatcacctta gcgtgcggcc tctggaagcc atcaatttaa ttcgtaatct gctgaaagtg
     4441 gatacgcatt tgattccaat gtcagagcat cctgttgatc tgatggcgat tgacgatcag
     4501 gggcatgaag tttacggcga ggtcaatatc gaccagttaa ctacgccgat tcaagagtta
     4561 ttgttaacgc ctaatgtacc cgcaacgcgt gaggcggttc acgctatcaa tgaagcggat
     4621 ctcatcatga ttgggcctgg cagtttttat accagcctga tgccaattct gctgctgaag
     4681 gaaatcgccc aggcattacg ccgcacgcca gcgccgatgg tttatatcgg caatctgggg
     4741 cgtgagttga gtttacctgc ggctaatttg aagctggaaa gcaagctggc aattatggag
     4801 cagtatgttg gtaaaaaggt cattgatgcg gtcatcgtcg ggccaaaagt ggatgtctcg
     4861 gcggtgaaag agcggattgt gatccaggag gtactggagg ccagcgatat tccgtatcgt
     4921 catgaccgcc agttgttaca taacgcgctg gaaaaggcgt tacaggcttt aggttaacgg
     4981 aaatccgaag gaaaattccg gcttcctatt gaagacaaag tgcgcgttgt ttatgccgga
     5041 tgcggcgtga acgccttatc cggcctacaa accgcgcaaa ttcaatatat tgcggagaaa
     5101 atgtaggcct gataagcgta gcgcatcagg ctgttttccg tttgtcatca gtcttcttcg
     5161 ctatcctgtt acgatgccgc gataaacagc tcacgcagct gatgcaactg gtcacgaatt
     5221 tgcgccgctt cttcgaactc cagattctgc gcgtgttgca tcatcaaccc ttccagctca
     5281 tggattttct gctgcaacgc tttaggcgac atatccatcg gcacattatc cggctcaaca
     5341 atcggtcgcg attttcctct gcccttcgct ttggttttag caatgttctg ccccaacgcc
     5401 aggatatcga ccactttctt gttcaagcct tgcggcgtaa tgccgtgttc ctcgttgtac
     5461 ttctgctgtt tctcgcggcg acgttcggtt tcgccaatcg ctttcgccat tgatggggtg
     5521 atcttatcgc cgtagagaat cgctttaccg ttaacgttac gtgccgcacg accaatggtc
     5581 tggatcaacg aacgttcgga acgcaggaag ccttctttgt cagcgtcgag gatcgccacc
     5641 agcgaaactt ccggcatatc cagaccttca cgcagtaagt tgatccctac caatacgtcg
     5701 aactcaccca gacgcaagtc gcggataatc tccatacgtt cgacggtgtc gatatctgag
     5761 tgaagataac gcacgcgctc gccatgttct tcgagatatt cggtaagatc ttccgccatc
     5821 cgcttggtca gtgtggtgac cagtacgcgt tcgttaattg ccgctcgctg acgaatctcc
     5881 gaaagaagat catcaacctg tgtcgccacc ggccgcactt cgataatcgg gtcaagcaat
     5941 ccggttggac gcaccacctg atccaccaca tcgccgccgg atttttccag ctcgtaatta
     6001 cccggcgtcg ccgaaacata gatggtttgc ggcgctaatg cttcgaactc ttcaaactta
     6061 agcggacggt tatccagcgc tgatggcagg cggaagccgt actccaccag tgtctcttta
     6121 cgcgcccggt caccgcgata catgccgcca atttgtggaa tggtgacgtg agattcatcg
     6181 accaccagca gcccatcagc agggaggtaa tcaaacagcg tcggcggtgg ctcacccggt
     6241 ccacgaccgg agaggaagcg cgagtagttt tcaatccccg aacagtagcc cagctcgttc
     6301 atcatctcca gatcaaactg ggtacgctgg gtcagccgct gctcttccag cagtttgttg
     6361 ttttccaaca gcactttgcg tctggcggcc agctcttctt tgatctcctc catcgcctgt
     6421 acgatgcgct cgcgcggtgt gacgtagtgc gttttcgggt agatggtaaa acgtggaata
     6481 gtggaaacaa tctgcccggt cagcgggtca aataacgaca atcgttccac ttcctcgtca
     6541 aacagttcca cgcgaagtgc aatgtcatcc gattctgccg ggaagatatc tatcacctcg
     6601 ccacgaacgc ggaaagtacc acgctggaat gcttgatcat tacgagcgta ttgcagctcc
     6661 gccagtcggc gcagaatcgc gcgctgatcg ataatcatac cgaccgtgag atggagcatc
     6721 atcttgagat ataaatcagg atcgcccaga ccataaatcg cggaaacaga cgccaccaca
     6781 accacatcac gccgctccag catcgctttg gtggcggaca aacgcatctg ctcaatatgt
     6841 tcgttaaccg aggcatcttt ctcaatgaaa gtgtcggaac tcggtacata ggcttccggc
     6901 tgatagtagt cgtagtagga aacgaaatat tccaccgcgt tttccgggaa gaactctttc
     6961 atttcgccat acagctgggc cgccagcgtt ttgttgggcg caagtaccat ggttgggcgc
     7021 tgaaggtcag caatgacatt ggcaatggtg aatgttttcc cggagccagt cacgccaagt
     7081 aacgtctggt gcgccaggcc atcttccagc ccctcttcga gacgtcgaat cgcctctggc
     7141 tgatcgccag aaggtttaaa agcggaattc agtttgaacg gtttactcat gagtcgctac
     7201 ctgaaggagt tgggcgggca ggtatgtaat tttactcgtc gtacttaatt atgccaacaa
     7261 attatactgg ataaaaaaac agttcatcac cataatattt ctgttacagc gtaaactccg
     7321 ctgtcaatca tgagcaaaat ttactctgtg gcgagataaa actccggcct taccgggtta
     7381 tccccaaagc aacggctttt ttaacatttg tcaagatgag tgatgacagt tttatggcaa
     7441 gagatgcctg ttcagtgact gccattgcat ttatctaacc agttaaaaaa taaaagatat
     7501 ttctttgagc cgtctttaac gccaattcgc gtgcaagccg cgtattctct cgcttgcctc
     7561 gtgttttcta actctaatac acatggttat ccacaggaat agt
//
LOCUS       DAZGVQ010000212         7560 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000212 DAZGVQ010000000
VERSION     DAZGVQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.214"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(145..4965)
                     /locus_tag="ACHLAG_004726"
     CDS             complement(145..4965)
                     /locus_tag="ACHLAG_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0657825.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LAAKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLVP
                     ALMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNINGKPAARAAVTLPPP
                     PPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITLTDSNAGFMDRVSA
                     GASLLFPAGPVLMEFATMVGGRGEIKKEVDFPEAGEDSALCDKENKPPRIAQGSSNVF
                     INNQPAARKGDKLECSAAIVEGSPDVFIGGEQVTYLDIQPEFPPWQRMILGGITIASY
                     LLPPAGLLGKLKNLARLGKLGNLLGKSGKLLGAKLGALLGKTGKSLKSIANKVIRWVT
                     DPVDPVSGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGWSDSWSEYAWVREQ
                     GNRVDIISQGATLRFAFDGDSDTAVNPYHAQYILRRRDDYLELFDRDALSSRFFYDAF
                     PGMRLRHPVTDDTDDDRLAHSPADRIYMLGGMSDTASNRITFERDSQYRITGVSHTDG
                     IRLKLTYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLDYHLFYEYDAADRI
                     IRWSDNDQTWSRFTYDEQGRCVTVTGAEGYYNATLDYGDGCTTLTDGKGTHRYYYDPD
                     GNILREAAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQLSRYTAADSAVWQ
                     YRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAYTPQGLLSGIFRQD
                     ERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGTGGQSVRLQWQQHHKLSG
                     IERAGTGAEGFRYDRHGNLLAWTDGNGVVWTMEYGPFDLPVARTDGEGHRWQYRYDKD
                     TLQLTEVINPQGESYRYILDNCGRVTEERDWGGVVWRYRYDADGLCTARVNGLEETIL
                     YSRDAAGRLAEVITPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYDERGRVNVTTQGRR
                     AIEYHYPDEHTVIRCILPPEDERDRHPDESLLKTTYRYNAAGELTEIILPGDETLTFS
                     RDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGLFPAEATWGGLLPSLVREYRYDSA
                     GNVSAVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDESYGYDSCGYLKAQ
                     SAGRHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTKHRDGYRPETERFRWDSR
                     DQLTGYCSAQGELWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSIAEIREYRDDKLYS
                     VRHLVFNGFELISQQFSRERQPHPSVAPQWVTRTNYAVSDLTGRPLMLFNSEGKTVWR
                     PGQTSLWGLALSLPADTGYPDPRGELDPEADPGLLYAGQWQDAESGLCYNRFRYYEPE
                     SGMYLVSDPLGLQGGEQTYRYVPNPLGYVDPLGLAVCPKLYEIYKGLRAQGFNPAEAR
                     DLAKWFNSSWGKADVYLRSAIGSGKIDYVGISRNVLNRYPQGKYDLELLTRLTGQLPR
                     NQARAIEQAIINAKRGNGFTNIQNSIDPAKDHFDAAVKWGEEWLKSNGFGGWLQ"
     gene            complement(4985..5446)
                     /locus_tag="ACHLAG_004727"
     CDS             complement(4985..5446)
                     /locus_tag="ACHLAG_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFP0657826.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5474..7375)
                     /locus_tag="ACHLAG_004728"
     CDS             complement(5474..7375)
                     /locus_tag="ACHLAG_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP0657827.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     SGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGAAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1414 a         2282 c         2015 g         1849 t
ORIGIN      
        1 gaataaaacc tatatcttgc atttctagtg taaaaagatc gcccacttca ggtctcttga
       61 atcttcgcac tcctttttta accattgaaa caatggagtc tgaacatatt tcgcttaatt
      121 ggttattcat taataccccc atttttattg cagccatcca ccaaatccat tactctttaa
      181 ccactcctca ccccatttta ccgctgcatc aaaatgatct ttagcaggat ctatactatt
      241 ctggatattt gtgaagccgt ttcctctttt agcattaatg atagcttgtt ctattgctct
      301 tgcttgattc ctcggaagtt gtcctgttag tcttgtgagt aactccaaat catatttacc
      361 ttgcggataa cgattaagca catttctact tattccaaca taatctattt ttcctgatcc
      421 tattgctgaa cgaagatata catcagcctt tccccagcta ctattgaacc attttgctag
      481 atctctagct tctgctgggt taaatccttg tgctcttaat cccttgtata tttcatacaa
      541 tttaggacat acagccaacc ctaacggatc aacatacccc agcggattag gcacataccg
      601 gtaagtctgc tcacccccct gcaaccccag cggatcactc accaggtaca tcccgctctc
      661 cggctcgtaa taccggaacc ggttatagca cagcccggat tccgcatcct gccactgtcc
      721 tgcatacagc aggccggggt cggcttccgg gtccagttcc ccgcgcgggt ccgggtagcc
      781 ggtgtctgcg ggcagactga gtgccagccc ccacaggctg gtctgccccg gtcgccagac
      841 ggttttacct tcactgttaa agagcatcag cggacggccc gtcaggtcgc tcaccgcata
      901 attcgttcgc gtcacccact gcggggccac cgacggatgc ggctgccgtt cccggctgaa
      961 ctgctggctt atcagctcaa agccgttaaa caccaggtgc cgtacgctgt acagtttatc
     1021 atcgcggtat tcccggattt ccgcaatact gtcgccgtcc cacaggtacg tgaagcggat
     1081 tttcttccgg tcgcagcgtt tctctgttcg tctgccgctg gcgtcgtggc ggtattccca
     1141 cagctccccc tgtgcgctgc aatacccggt cagctggtcc cggctgtccc accggaaccg
     1201 ctctgtttcc gggcggtagc cgtcacggtg ttttgtccgg ctgaccatcc ggcctgcggc
     1261 gtcatagtca tactgcgtgt ttccggcttg tttcagccgg tggcctccgg catactggtc
     1321 agtctcttca cttatccggt gcctgcccgc agactgcgcc ttcaggtagc cacaactgtc
     1381 atagccatag gactcgtcgc cttcgccata gcccagcccg gtgcctgagg ctgtcaccgc
     1441 cgtgacctgg ccgttccggt ccagccggta ctcccgccgt gtttcccgtc cgtaatcttc
     1501 ccggctggtg acagccgaca cattacccgc gctgtcgtaa cggtactccc gtaccagtga
     1561 ggggagcagc ccgccccatg tggcttctgc cgggaaaagt ccggcgcgct ggctgacagg
     1621 ctgaccggct gcattccagc cctgttcaca ggcaaaaccc cggttactgt gccggagcac
     1681 ttcacgtccc gcctcatcac ggctgaacgt cagcgtctca tcccccggca ggataatctc
     1741 cgtcagttct ccggcggcgt tgtagcggta cgtggttttc agcagggatt catcggggtg
     1801 tctgtcgcgt tcatcttccg gtggcaggat acagcggatg acggtgtgtt cgtcggggta
     1861 gtggtattca atggcccgtc ggccctgagt ggtgacattc acccgcccgc gttcgtcgta
     1921 gccggtgcgc tgtgatgtgc cgtccgggct gaagataccc gtcagcctgc cggatttgtc
     1981 ataggcatac tgcgttttgc cttccggggt gatgacttct gccaggcggc ctgcggcgtc
     2041 ccggctgtag aggatggttt cctccaggcc gttgaccctg gcggtacaca ggccatcggc
     2101 gtcatagcgg taacgccaga ccacgccgcc ccagtcacgc tcttccgtca cccgtccaca
     2161 gttgtccaga atataacggt atgactcgcc ctgcggattg atgacttctg tcagttgcag
     2221 cgtgtcttta tcgtagcggt actgccagcg gtggccttca ccgtccgttc gcgccaccgg
     2281 caaatcgaac gggccgtatt ccatcgtcca gacaacgccg ttgccgtccg tccaggccag
     2341 caggttgccg tggcggtcat agcgaaatcc ttccgcaccg gttccagcac gttcaatgcc
     2401 gctcagttta tggtgctgct gccactgtaa ccgcactgac tgaccgccgg tgccgcgcat
     2461 cttcaccggc aggtcgtgac cactgtattc ggtgtggtgt tcacggccca tcacgtcggt
     2521 gagtgtttcc ggccggttgt ggtggtcgta ctctatgccc agacgccgtt catcctggcg
     2581 gaatatcccc gacagcagcc cctgcggcgt gtacgccagc cgggtctctt cgccctgtgg
     2641 cgacaccagg cttaccggca ggccgcgttc atcacactgc tgtgtccacg tctgcccggc
     2701 tgggtcggtg atgttgctga gcaggccgcg ctcatcatag cggtactgcc acaccgcgct
     2761 gtctgccgcc gtataacggc ttaactgacc gtgtgcggcg ttgtattcaa acttctccac
     2821 ccgcccggca ggggagtggc gggccagcag gtgatggaat tcatcccatt catacgtggt
     2881 ggtgctgccg tccggcgctg cttcccgcag aatattgccg tcaggatcat agtaataacg
     2941 gtgagtgccc ttgccgtccg tcagggtggt gcagccgtca ccatagtcca gcgtggcgtt
     3001 gtaatagccc tccgccccgg tgacggtcac gcaccggccc tgttcatcgt aggtgaaacg
     3061 gctccacgtc tggtcgttat cggaccagcg gatgatccgg tccgcagcgt cgtactcata
     3121 aaacaggtgg tagtccagcc gcgcatccgc ttcggtcagc cgcccccgcg catcctgttc
     3181 gtaggtcgcc agcgtctgta tgccgttatc cgtgcggtga atggctttca ggtagccgct
     3241 ggcatggtac gtcagtttaa gccggatccc gtcggtgtga ctgacacccg tgatccggta
     3301 ctggctgtcg cgctcaaacg tgatgcggtt gctggcggtg tcgctcatcc cgcccagcat
     3361 gtatatccgg tctgcggggc tgtgtgccag acggtcatcg tcggtatcgt cagtcaccgg
     3421 gtggcgcagg cgcattcccg gaaaggcgtc ataaaagaag cggctgctca gggcatccct
     3481 gtcgaacagc tccaggtaat catcgcggcg gcgcagaatg tactgggcgt gatacgggtt
     3541 aaccgccgta tcactgtcac cgtcgaaggc aaatctcagc gtggctccct ggctgatgat
     3601 atccacccgg tttccctgtt cacgcaccca ggcgtattcg ctccaggagt cgctccagcc
     3661 cacgcccgtc aggccatgca gtggcagtac agaactgtgg aaacgggtga aggagagggg
     3721 gagggtctgg ccaagggtga agtcggtacg ttcgtcgcag tatgcgccgc ttaccggatc
     3781 gacaggatct gttacccatc tgatgacttt attggcaata ctttttaacg acttacctgt
     3841 tttccccagc aacgcgccga gctttgcgcc cagcagcttc ccgcttttcc ccagcaggtt
     3901 tcccagtttg ccgagtctcg ccagattctt cagttttccc agcagtcccg ctggcggcag
     3961 aagatagctg gctatcgtta ttcctcccag gatcattctc tgccatggcg ggaactccgg
     4021 ctggatatcc agataggtga cctgctcacc cccaataaag acgtccggcg aaccttccac
     4081 gattgccgcg ctgcactcca gtttgtcgcc cttgcgcgcg gcaggctgat tgttgataaa
     4141 gacgttgctg ctgccctggg ctatcctcgg tggtttgttc tccttgtcgc agagcgccga
     4201 gtcctcaccg gcttccggga aatccacctc ttttttgatt tcgccacgcc cgcccaccat
     4261 ggtggcaaac tccattaata ccggaccggc gggaaacaga agcgatgcgc cggcgctgac
     4321 gcgatccata aaaccggcat tgctgtccgt cagggtgata acagcgttcc cccaggcctt
     4381 cgccagtggt actgcttttt ccgctgccga ttcaagcagg tcagtggcaa tatcacccca
     4441 tgagggttcc ccctgtggga cttcaggtat tgctccaggt gggggaggag ggggaagggt
     4501 gacggcggcc ctggcagcgg gctttccgtt aatgtttaca ttttcagaac cggtattgat
     4561 ggcgccgtcc gtgtcacaaa agctgtttat ccatgactca agctggttct gtttctcctc
     4621 cacataatca ctcgccttat agctggcata catcagcgca ggcaccagaa ccagcgcata
     4681 agggccggtg gcacaggcgg ccataatcag gcaaccttct gcgatggtca ctatcgcccc
     4741 cagcacggcc cctgccagtg cccccagaaa acttttatgc tgaatttcat cgcccagcct
     4801 ggcggcaggt gggccggact tctttgctgc cagcatctgt gcaatggctt tgtcgttggc
     4861 tgcccgtttc atctggctgg cgaccatcgc ctgctgtgac agcattcgca ttgccagcgt
     4921 accgcctgcg gtcgctccct gtggcccgcc tggtccttca ctcatgtgtt atccctcgtt
     4981 tatgtcagcg cggctggtcc ttttggatgt cattctccgg tggcaacggg gtgaagctct
     5041 ttatcacctc cagcagcatc gtcttttgac tgttcttcag ggataaggat gtgctgaaag
     5101 tgaaggtcag cagcgtatct ccacgccgct ggaatacggc tatctggcta atccgcccct
     5161 gattcccctc atcttccgga ccgttccagg aataatccag cagccaggcc ggggcctctg
     5221 ccagagtgat ttcccggcag gcatgttcgg tgtacccatc aagtttgccc tggtacagcg
     5281 ccagcgtttc ctggtacagc tctggtgtca gggttccctg tggaacggct tcgcgggtaa
     5341 tgaccaggtt gatgccactg ttatctgtgg acaccaggac ctgcattgac tgatcctgcc
     5401 attgagacgg cagttgaatg tggccttcat taaaagtgaa ctgcataaaa tactccctga
     5461 taatctgagt gaatcagttg atgaaaacag aaccgcccac gatggtgatg ccatccttcg
     5521 acagcctgat taccgtcgat tccccgacct gtagctgaat gccatccggc gtcagcgcaa
     5581 ttttgctttc ctccacagcc agcaaaattt ccttaatcgc cgtataagcc tgtataccgt
     5641 cgccttcgcc accattgttt acagcgtcaa catgcaccga accggcaatc gtgcctctgt
     5701 cctgaaagac atcctcagat aaagaccctt tcagtactcc ggtgatcttg tggccttttt
     5761 ccaccgtaag aaggcggtta ccttcctgga tcgtgaccgt ctggtcattt tttacggttt
     5821 cgcggtgatc gtgttttacg gtggtggtac ggtcgttgag cacttcggta tccatgttct
     5881 tctgcgcgtg gatgtagacc tgttctctcc ctgtcgcatc atcaaacttc agctcattaa
     5941 atccgctgcc catgtacgtt ttggagcgga tggtcatctg cgtctttgtt cccggcaggc
     6001 tgccgggggt gcggttttcc tggtggtagg tgcgccccat aatgatcggc tggtccggat
     6061 cgccgttgag gaagtctaca atcacctcct gacccacacg cggtatcgcc aggtggccaa
     6121 aaccggtgcc tgcccacgcc tgtgccacac ggatccagca cgaactgtct tggtctgccg
     6181 ggttataacg gtcccagttg aacttcaccc gcacgcgacc atgttcgtcg cagaagattt
     6241 cctcgcctgc cgctcctgtc acgacagcgc tctgcgggcc gtccaccagc ggtttcagca
     6301 acggctgtgg tcgccatgtt ctgtctgccg ggatcaccgc aaagtgatta ttcagcgtgg
     6361 tgcctgaacc actgcgtccc ggcaccgcct gtggctgttc gccgtgcagt tcacttgcca
     6421 ccacctgcca ttcccggttc aggttcgcct gcggatgccc cgtcagcaca attcgccgtc
     6481 ccggccatat ctccggcgag cggctcattc cccgcgcggt ttctgcgttg tttcgccagc
     6541 cgtccatctg ccagcgggca aagttctgcc cgtgggcgct cttgaaacgt ccggggtagt
     6601 catacacttc atactgcgtg cgctggtagt cctggtgctg gccttcctgt tcaaaacgtc
     6661 cggcccagcc ggggcgttta aaggtgtagt ctttggtcac cacggaagaa gggcggattt
     6721 gtgcgctgta gcggaactgg ctgatgcaga gggtgctcac ctcggtacgg gtgttcgggt
     6781 tccatgggat ttcaaaagat tcgggcagat ggcggactgt gtcgcacagc accaggctct
     6841 ggtcggtact tttgtaagca tgctcctcat aaaagaagat gccttcctcc gccgccatcc
     6901 ggcacaggaa atcgtaatca gtctcaccgt actggacaca aaactcacgg gaaggatgcg
     6961 gctcgctgaa cagcggactc cattcggtca ccccgttttc ctgcaacatc gtgccgagga
     7021 tgcttttgat gtcctcgttc tggaagatac ggaagttctg gcgcagaccg gcacgccaca
     7081 gcggcgggtg caccttcatg ctgtacagca tctggttttt gtcgttctcc ccgagttcaa
     7141 accaggtcac cacgcctttc acccggcgct gtacttcatc gccctgccat atcgtcaggt
     7201 aggccatttt gtccagcacc tgcgcaaatt caagggagag aaactgctgg ctgaccaggg
     7261 agagatcgag ggaaaagagc gaagagagtg actggttcag atgaaaggaa accaccgcaa
     7321 aagcatccgg cggcaggccg tccacttcca gtgtgaaacg taatccggtt gacatagcaa
     7381 cctccagtaa aatgaataat gatccgtacc gtacaactgc ggataatgtg ataagcggga
     7441 cgacttcaga cgttattagg gaaggaacag aaaatgctat ggttatgtga gaattttgtt
     7501 taaatattac tttacagaaa cgggtgtctg ttcagtcaaa aaaataatca caagctcaac
//
LOCUS       DAZGVQ010000213         7551 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000213 DAZGVQ010000000
VERSION     DAZGVQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.215"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..300)
                     /gene="yqfB"
                     /locus_tag="ACHLAG_004729"
     CDS             complement(<1..300)
                     /gene="yqfB"
                     /locus_tag="ACHLAG_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFP0657828.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVRLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEF"
     gene            345..1778
                     /gene="bglA"
                     /locus_tag="ACHLAG_004730"
     CDS             345..1778
                     /gene="bglA"
                     /locus_tag="ACHLAG_004730"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFP0657829.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGSWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            complement(1835..2578)
                     /locus_tag="ACHLAG_004731"
     CDS             complement(1835..2578)
                     /locus_tag="ACHLAG_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP0657830.1"
                     /translation="MVIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(2839..5712)
                     /gene="gcvP"
                     /locus_tag="ACHLAG_004732"
     CDS             complement(2839..5712)
                     /gene="gcvP"
                     /locus_tag="ACHLAG_004732"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFP0657831.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(5831..6220)
                     /gene="gcvH"
                     /locus_tag="ACHLAG_004733"
     CDS             complement(5831..6220)
                     /gene="gcvH"
                     /locus_tag="ACHLAG_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFP0657832.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(6244..7338)
                     /gene="gcvT"
                     /locus_tag="ACHLAG_004734"
     CDS             complement(6244..7338)
                     /gene="gcvT"
                     /locus_tag="ACHLAG_004734"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFP0657833.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
BASE COUNT         1731 a         2065 c         1962 g         1793 t
ORIGIN      
        1 aaattcaatc acataaaact gtgtctgacc gggatagatg tcggcaatga cctttttcag
       61 ttcagtcagg gtcatatttt cctgctctgc atgtttttca gtcagcgtat ccagccttac
      121 ggttgaggtt gcggtgactt caatcgtgca aaaataaccg tcatcttcaa aacgcccgac
      181 acgaagcaca tcacccgttt tgaagtgcga ttcagactcg tcgcggatgg tgatggtttt
      241 acgcccagcc agaatgtcat cctggaaacg ttgaaaaaaa gtgatgtcgt ttggctgcat
      301 ggtactattt cctgtaagaa ttgactcatc tggagcctat gatagtgaaa aaactcacct
      361 taccgaaaga tttcttatgg ggcggcgcag ttgccgctca tcaggtcgaa ggcagctgga
      421 acaaaggcgg caaagggccg agcatttgtg acgttctgac cggtggcgca cacggcgtgc
      481 cgcgcgaaat caccaaagaa gtcttgccag gcaaatacta tccaaaccat gaagccgttg
      541 atttttatgg tcactacaag gaagacatca agctatttgc cgaaatgggc ttcaaatgtt
      601 ttcggacttc catcgcctgg acgcgtattt ttccaaaagg tgatgaagct cagccaaacg
      661 aagaagggct gaagttctac gatgatatgt tcgatgaact gctgaaatac aacatcgaac
      721 cggtgatcac cctctcccac tttgaaatgc cgctgcatct ggtgcagcaa tacggtagct
      781 ggaccaaccg taaagtggtt gatttctttg tccgtttcgc ggaagtggtg tttgaacgct
      841 ataagcataa agtcaaatac tggatgacct tcaacgaaat taacaaccag cgtaactggc
      901 gtgcaccgct gttcggttac tgctgctccg gcgtggttta taccgagcat gacaacccgg
      961 aagagacgat gtaccaggtg ctgcatcacc agtttgtcgc cagcgccctg gcggtgaaag
     1021 ctgcgcgtcg cattaacccg gagatgaaag tcggctgtat gctggcgatg gtgccgctct
     1081 atccctactg ctgtaacccg gacgatgtga tgttcgctca ggagtcgatg cgcgaacgct
     1141 acgtctttac cgatgtgcag ctacgcggct attacccgtc ctatgtgttg aacgagtggg
     1201 agcgtcgcgg atttaacatc aaaatggaag acggcgatct cgatgtactg cgtgaaggca
     1261 cctgcgatta tcttggtttc agctattaca tgaccaacgc agtgaaggcc gaaggcggca
     1321 ccggcgatgc gatctctggt ttcgaaggca gcgtaccgaa cccgtatgtt aaagcgtctg
     1381 actggggctg gcagattgat ccagtaggtc tgcgctatgc cctttgcgaa ctgtatgagc
     1441 gttatcagag gccgctgttt attgtcgaaa acggttttgg cgcttacgac aaagtggaag
     1501 aagatggcag catcaacgac gactaccgca ttgactacct gcgcgcccat atcgaagaga
     1561 tgaaaaaagc ggtgacttac gatggcgtgg atctgatggg ctacacaccg tggggctgca
     1621 tcgactgcgt atcgttcacc accgggcagt acagcaaacg ctacggcttt atctatgtga
     1681 ataaacatga cgacggtgct ggcgatatgt cgcgttcacg taagaagagc tttaactggt
     1741 accaagaggt gattgccagc aacggcgaga agctttaagt cgatgaagta ccggatgcaa
     1801 tacttgttgc atcaggtcag acaacttccc tgttttattt cccgcccgcc aaatcgataa
     1861 aacttcccgt gacgtaagag gctttatcac ttagtagcca gacaatggcc tgcgcgacct
     1921 cttctgcctg tccaccacgc tgcatgggga tgttcgactt aacgcgatcg acgcgtccag
     1981 gctcgccgcc gctggcgtgc atttcggtat aaataaaccc tggccgcacg cagttaacgc
     2041 ggatcccctg cgcggcgact tccagcgata gtccggtggt taacgtatca atcgcccctt
     2101 tcgatgccgc gtaatcaaca tattcccctg gcgaacccaa ccgtgaggcc accgaagaga
     2161 cattgacgat agcgccgcca ctgccaccat ttttaagcgc catgcgtttt accgcctcgc
     2221 ggcagcagag aaaatatccc gtcacgttgg tggaaagtac tcggttgatt cgctctgcgg
     2281 taaggttttc aacggtgcac tgggtaaaca agatcccggc gttattgacc agcgctgcta
     2341 gcggttcatc gtgctgatcg attgctgtaa acatcgcaac gacctggttt tcgtcgctga
     2401 tatccgcctg gagcacgaat gctttgccac cggcttgcgt tattaagttc atcacttcct
     2461 gcgccgcgtg gaggttttgc tgataattaa ccgccaccgt atacccttct tgcgccaaca
     2521 gtaatgcagt tgcccgcccg atgccgcgac tgccaccggt cacaagtgct ataaccattt
     2581 gtttttccga gaaaaattca ttcgttgacg tatctttgag attgctaaca aactggctca
     2641 atcttcaatg ttggcacgct tttccccctc gccctttcag ggagagggcc ggggtgaggg
     2701 taaatattcg cgccagtgcc ggcctgttcc cctcaccctc tccccaaagg ggcgagggga
     2761 ctgtctgagc acttttgtac tttgtcatct gactaaaaag gcgccgaagc gcctttagaa
     2821 aatagtcgaa tcagtgaatt actggtattc gctaatcggt acgcaggagc agaacaggtt
     2881 acggtcgccg taaacatcat ccagacgttt caccgtcggc cagtatttgt ctgccacacc
     2941 tgccgggaat actgcaacct cacggctgta cggatgcgcc cactcggcga ccagttcgtt
     3001 ctgaatgtgc ggcgcgttca ccagcgggtt atcttccagc ggccagacac cggctttaac
     3061 ctggtcgatt tctgcgcgga tagccagcat cgcgtcgata aagcgatcca gttccacttt
     3121 gctttcagat tcagtcggtt caaccatcag cgtacccgcc accgggaacg acatggtcgg
     3181 cgcgtggaaa ccgtagtcga tcaggcgctt ggcaatatcc agctcgctga tgccggtttc
     3241 ttctttcagc gggcgaatat cgagaataca ttcgtgcgcc acgcgaccgt cgcgaccggt
     3301 atacagcacc gggaaggcat cctgcaggcg gctggcaata tagttggcgt tgaggattgc
     3361 cacctggctt gcttttttca gcccttctgc gcccatcatg cggatgtaca tccagctgat
     3421 tggcaggata gaggcgctac cgaacggtgc cgcagaaact gcgccctgac gggttaacat
     3481 gccttcgatt tgcaccacgc tatgacccgg tacaaacggt gccagatgcg ctttcacacc
     3541 gatcggtccc atacccggac caccaccgcc gtgcggaatg cagaaagttt tatgcaggtt
     3601 gaggtgcgaa acgtccgcgc caataaagcc cggcgaggtg atgccaacct gggcgttcat
     3661 gttcgcgcca tcaaggtaaa cctgaccgcc gaactgatgc acgacttcac acacttcacg
     3721 gatcgtttct tcatacacgc cgtgggtaga aggataagtc accatgatac aggagaggtt
     3781 atcgcccgcc tgttccgctt tcgcgcgcag atcagtcaga tcgatgttgc cgtttttatc
     3841 acacgccaca accaccacct gcattcctgc catatgtgca gaagcggggt tagttccgtg
     3901 cgcagaagcc gggatcaggc agatatcgcg atgcccttcg ttgcggcttt catgataatg
     3961 acgaatcgcc agcaggcccg cgtattcgcc ctgtgcgccg gagttcggct gcatacaaac
     4021 ggcgtcgtaa ccggtcagtt tcaccagcca gtcagccagc tgcgcaatca tctgctgata
     4081 accttcagcc tgttccggcg gacaaaacgg gtgcagttcg gcaaattccg gccaggtgat
     4141 tgggatcatc tcggcggcgg cgttcagttt catggtgcag gaacccagcg ggatcatcgc
     4201 ctgattcagc gccagatctt tacgctccag cgagtgcata tagcgcatca tttcggtttc
     4261 gctgtggtag cgattaaaca ccggatgggt gaggatttcg tcgtcgcgca gcatcgcagg
     4321 ctggatagag cggctgtcgt gagccacgtc tttgtccagc gtgtcgatgt ccaggccgtg
     4381 gttatcgccc agcagcacgt tgaaaagctg cattacgttt tcacgcgtgg ttgtttcatc
     4441 aagggtgatc ccaaccgcgt tcagaatatc gctacgcagg ttgatttcag ccgcttcggc
     4501 acgcgtcagt acgcccgctt tgtcggccac ttccacacac aaggtgtcga aatagtgcgc
     4561 atggcgcagt ttcagacctt tttgttgcag gcccgccgcc aggatatcgg tcagacggtg
     4621 aatgcggtta gcgatacgtt tcaggccaat cgggccgtga taaacggcat acaggctggc
     4681 gatgtttgcc agcagcacct gggaagtaca aatgttggag ttcgctttct cacggcgaat
     4741 atgttgctcg cgagtctgca tcgccatgcg cagcgcggta ttgccagctg catctttcga
     4801 tacaccgata atacggcccg gcattgagcg tttgtattca tctttcgccg caaagaaggc
     4861 cgcgtgtggg ccaccgtagc ccatcggcac gccgaagcgt tgcgccgaac caaaaacaat
     4921 atccgcgccc tgtttacccg gcgcagttaa cagcaccagc gccataatat cggcggcgac
     4981 actgaccaca attttgcgtg atttcagttc gctaataagc gcggtgtagt cgtggatttc
     5041 accggttgtg ccaacctgct gtaacagcac gccgaagacg tcctgatggt cgagcacttt
     5101 ttgcgcgtca tcgacaatca cttcaaaacc aaaggtttcg gcacgagtac ggaccacatc
     5161 cagcgtttgc ggatgcacat cggaagccac gaagaagcgg ttggcatttt tcagtttgct
     5221 gacgcgtttc gccatcgcca ttgcttcggc ggcagcggtg gcctcgtcca gaagagaagc
     5281 agaggccata tccagtccag tcaaatccag cgttacctgc tggaagttga gcagtgcttc
     5341 aaggcggccc tgggagactt caggttgata cggagtgtac gcggtatacc agcccggatt
     5401 ttccagcatg ttacgcagga taaccggcgg tagctgcacg gcggtgtaac ccatgccgat
     5461 gtaagacgtg aagcgtttat tgcgactggc aatagccttg agttctgcca gtgcggcgta
     5521 ttcggtcgcc ggtgcgccaa cctgcggcgg ggtcgcaagc tgaatatctt tcggcacaat
     5581 ctggccggtc agcgcgttta acgattgtgc accaacggca ttcagcattt cttgctgttg
     5641 cgcggcgtcc ggtccgatat ggcgttcaat aaaagcgccg ctgttttcaa gctggcttaa
     5701 cgtctgtgtc atgagcgatg gttcctgaaa cgtgcagtga attgtgaacc tctctcctta
     5761 cgaagagagt gagggtgagg cataaatttt cctcaccctg atcctctccc gcagaagagg
     5821 aataaagccg ttactcgtct tctaacaatg cttcgtatgc ggtcgcatcc agcagtgatt
     5881 ccagttcgct ttcatcgctg gctttgattt taaagatcca gccacctgca tacggttcgc
     5941 tgttcaccag ttccggggaa tcgctcagtg cgtcatttac cgccacgatt tcaccgctta
     6001 ctggcgcata aatgtctgac gccgctttta ctgattcggc aaccgcgcag tcatcgcccg
     6061 cgctaaccgt tgcgcccact tccggcaggt caacaaacac catatcgcct aacagctcct
     6121 gggcatgttc ggttatacca acggtgtaag tgccgtcggc ttctttacgc agccattcgt
     6181 gttctttgct gtatttcagt tctgctggta cgttgctcat caatcaatct ccaaaaaagt
     6241 aaatcacgcg acggctttac cgttacgcac aaaaacaggt ttcgtcactt taaccggcat
     6301 ttcacggttg cgaatttgca caatcgccgt ttcgccaata ccttccggca cgcgtgccag
     6361 cgcaatgctg taacccagcg tcggggagaa agtcccgctg gtgataatgc cttcatgctg
     6421 gttgccctgc gcatcggtaa aacgtaccgg cagttcatta cgcagcacgc ctttttcggt
     6481 catcaccaga ccaaccagtt tttctgtacc atgctcacgc tgcacttcca gggcttcacg
     6541 accgataaag tcacgatctg ccggttccca ggcgatagtc cagcccatgt tggcggctaa
     6601 aggagaaata gtttcgtcca tctcctgacc ataaagattc atgcccgctt ccagacgcag
     6661 cgtgtcacgc gcgcccaagc cacatggctt aacacccgct tccaccagcg cacgccagaa
     6721 atcggccgct ttttcattgg gcagcgcaat ttcatagccc gcttcaccgg tataaccagt
     6781 ggtggcaata aacagatcgc ccgcctgcac gccaaagaac ggtttcatcc cttccaccgc
     6841 ctgacgctgg gcgtcattaa acagtgtggc agcttttgcc tgcgcattcg gcccttgcac
     6901 ggcaatcatg gaaaggtcat cacgaacggt aatttcgatg ccgaaaggtt cagcgtgttg
     6961 ggtaatccag gagaggtctt tttcgcgggt ggcggagtta acaacgaggc ggaagaaatc
     7021 ttcagtaaag tagtagacga tgaggtcatc tatcacaccg ccagaggcat tcaacatccc
     7081 cgagtaaagg gctttgccgc ttttggtgag cttcgccaca tcgttcgcca gcagataacg
     7141 cagaaactcc cgggtgcggc tgccgcggag atcgacgatg gtcatatgtg acacatcaaa
     7201 cattccggca tcggtacgta ccgcatgatg ttcgtcgatt tgcgaaccgt aatgcagcgg
     7261 catcatccag ccgtggaaat ccaccatgcg agcgccgcaa agcgtgtgtt gttcgtacaa
     7321 aggagtctgt tgtgccatct tgtcctcatt gaataagcgg ggctgacaac ttttcatggt
     7381 gaaattatca ccacgaaacc cagcatcgga gccactcccg gtccccaacg caatcgttct
     7441 cttttgcctg aacttaccac cgaaacagac tgttaaccat aaggtaaaat tgatcatcac
     7501 attagcttat ggttaaaaaa cgccaaaatc gcgacagaat aaaaaaccaa a
//
LOCUS       DAZGVQ010000214         7549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000214 DAZGVQ010000000
VERSION     DAZGVQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.216"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            226..4472
                     /gene="fdeC"
                     /locus_tag="ACHLAG_004735"
                     /pseudo
     CDS             226..4472
                     /gene="fdeC"
                     /locus_tag="ACHLAG_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
     gene            complement(4594..5451)
                     /locus_tag="ACHLAG_004736"
     CDS             complement(4594..5451)
                     /locus_tag="ACHLAG_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0657834.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMAKEWTINRG
                     LYASFRDEGEWERYPEWSRNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            5700..6566
                     /locus_tag="ACHLAG_004737"
     CDS             5700..6566
                     /locus_tag="ACHLAG_004737"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP0657835.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKNS"
     gene            complement(6730..7323)
                     /gene="rclC"
                     /locus_tag="ACHLAG_004738"
     CDS             complement(6730..7323)
                     /gene="rclC"
                     /locus_tag="ACHLAG_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFP0657836.1"
                     /translation="MEKYLHMLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(7335..>7549)
                     /gene="rclB"
                     /locus_tag="ACHLAG_004739"
     CDS             complement(7335..>7549)
                     /gene="rclB"
                     /locus_tag="ACHLAG_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFP0657837.1"
                     /translation="ATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLEDAIADIAR
                     KYNASSWKVTSMRIDNNSTATAVLYK"
BASE COUNT         2066 a         1682 c         1929 g         1872 t
ORIGIN      
        1 aaaagctaaa ttttcaccca taaataaaaa tataatcccg cgcccaacca cctgatgagt
       61 ggctacagac actggatata ttaggtggcg gtgcactttc ttacataaag gtatttcctt
      121 ttctgcggaa aaggaaatcg ggaaatcccc ggtttttctg acaagcagac gccattattt
      181 gtgtctgcct atgttcgtta attcgttcat caggaaatta tctcaatgtc gcgttataaa
      241 acaggtcata aacaaccacg atttcgttat tcagttctgg cccgctgcgt ggcgtgggca
      301 aatatctctg ttcaggttct ttttccactc gctgtcacct ttacccagta atggcggcac
      361 gtgcgcagca tgcggttcag ccacggttga gcatgggaaa tactacggta actgctgata
      421 ataacgtgga gaaaaatgtc gcgtcgtttg ccgcaaatgc cgggacattt ttaagcagtc
      481 agccagatag cgatgcgaca cgtaacttta ttaccggaat ggccacagct aaagctaacc
      541 aggaaataca ggagtggctc gggaaatatg gtactgcgcg cgtcaaactg aatgtcgata
      601 aagatttctc gctgaaggat tcttcgctgg aaatgcttta tccgatttat gatacgccaa
      661 caaatatgtt gttcactcag ggagcaatac atcgtaccga cgatcgtact cagtcaaata
      721 ttggttttgg ctggcgtcat ttttcaggaa atgactggat ggcgggggtg aacaccttta
      781 tcgaccatga tttatcccgt agtcataccc gcattggtgt tggtgcggaa tactggcgcg
      841 attatctgaa actgagcgcc aatggttata ttcgggcttc tggctggaaa aaatcgccgg
      901 atattgagga ttatcaggaa cgcccggcga atggttggga tatccgcgca gagggctatt
      961 tacctgcctg gccgcagctt ggcgcaagcc tgatgtatga acagtattat ggcgatgaag
     1021 tcgggctgtt tggtaaagat aagcgccaga aagacccgca tgctatttct gccgaggtga
     1081 cctatacgcc agtgcctctt ctgacactga gcgccgggca taagcagggc aagagcggtg
     1141 agaatgacac tcgctttggc ctggaagtta attatcggat tggcgaacct ctggagaaac
     1201 aactcgatac agacagcatt cgcgagcgtc gaatgctggc aggcagccgc tatgacctgg
     1261 ttgagcgtaa taacaacatc gttcttgagt accgcaaatc tgaagtgatc cgtattgctc
     1321 tgcctgagcg tattgaaggt aagggcggtc agacactttc cctggggctt gtggtcagca
     1381 aagcaactca cggactgaaa aatgtgcagt gggaagcgcc gtcattactg gctgaaggtg
     1441 gcaaaattac cggtcagggt agtcagtggc aagtaacgct cccggcttat cgtccaggca
     1501 aagacaatta ttatgcgatt tctgcggttg cctacgataa caaaggcaat gcctcaaaac
     1561 gcgtgcagac agaggtggtc attaccggag caggtatgag tgccgatcgc acggcgttaa
     1621 cgcttgacgg tcagagccgt attcaaatgc ttgctaacgg taatgagcaa agaccgctgg
     1681 tgctgtctct gcgcgacgcc gagggccagc cagtcacggg catgaaagat cagatcaaga
     1741 ctgaactaac tttcaaaccg gctggaaata ttgtgactcg taccctgaag gccactaaat
     1801 cacaggcaaa gccaacactg ggtgagttca ccgaaactga agcaggggtg tatcagtctg
     1861 tctttactac cggaacgcag tcaggtgagg caacgattac tgttagtgtt gatggcatga
     1921 gcaaaaccgt cactgcagaa ctgcgggcca cgatgatgga tgtggcaaac tccaccctga
     1981 gcgctaacga gccgtcaggt gacgtggttg ctgatggtca gcaagcctac acgctgactg
     2041 cggtggatac tgatggtaac ccggtgacgg gagaggccag ccgcttgcga tttgttccgc
     2101 aagacactaa tggtgtcacc attggtacaa tttcggagat aaaaccaggc gtttacagcg
     2161 ctacggtttc ttcgacccgt gccggaaacg ttgttgtgcg tgctttcagc gagcagtatc
     2221 agctgggcac attacaacaa acgctgaagt ttgttgccgg gccgcttgat gcagcacatt
     2281 cgtccatcac cctgaatcct gataaaccgg tggttggggg tacagttacg gcaatctgga
     2341 cggtaaaaga tgcctatgac aaccctgtga ccagcctcac gccggaagcg ccgtcattag
     2401 cgggtgccgc tgctgtaggt tctacggcat cgggctggac aaataatggt gatgggacgt
     2461 ggactgcgca gattactctc ggctctacgg cgggtgaatt agaagttatg ccgaagctaa
     2521 atggacagga tgcggcagca aatgcggcaa aagtaaccgt ggtggctgat gcgttatctt
     2581 caaaccagtc gaaagtctct gtcgcagaag atcacgtaaa agccggcgaa agtacaaccg
     2641 tgacgctggt ggcgaaagat gcgcatggca acgctatcag tggtctttcg ttgtcggcaa
     2701 gtttgacggg gaccgcctct gaaggggcga ccgtttccag ttggaccgaa aaaggtgacg
     2761 gttcctatgt tgctacgttg actacaggcg gaaagacggg cgagcttcgt gtcatgccgc
     2821 tcttcaacgg ccagccagca gccaccgaag ccgcgcagtt gacggtcatc gccggagaga
     2881 tgtcatcagc gaactctacg cttgttgcgg acaataaggc tccgaccgtc aaaacgacga
     2941 cggaactcac cttcaccgtg aaggatgcgt acgggaaccc ggtcaccggg ctgaagccag
     3001 atgcaccagt gtttagcggt gccgccagca cggggagtga gcgtccttca gcaggaagct
     3061 ggacagagaa aggtaatggg gtctacgtgt cgaccttaac gctgggatct gccgcgggtc
     3121 agttgtctgt gatgccgcga gtgaacggcc aaaatgccgt tgctcagcca ctggtgctga
     3181 acgttgcagg tgacgcatct aaggctgaga ttggtgatat gacagtgaag gttaataacc
     3241 aactggctaa tggacagtct gctaaccaga taaccctgac cgtcgtggac agctatggta
     3301 acccgttgca ggggcaagaa gttacgctga ctttaccgca gggtgtgacc agcaagacgg
     3361 ggaatacagt aacaaccaat gcggcaggga aagtggacat tgagcttatg tcaacggttg
     3421 cgggagaaca caatatttcc gcttcggtga atggtgctca gaagacggtc acggtgaaat
     3481 ttaacgcgga tgccagcacc ggtcaggcaa acctgcaggt agacaccgcc gttcaaaaag
     3541 tggcaaacgg caaagacgcc tttacgctga cggcgaccgt tgaggataaa aatggtaacc
     3601 ctgttccagg gagcctggtg acctttaatc tgccccgggg tgtcaagccg cttacaggcg
     3661 ataatgtctg ggtgaaagcc aacgatgagg ggaaagcaga gttgcaggtg gtttcagtga
     3721 ctgccggaac ctatgagatc acggcgtcgg caggaaatga ccagccttcg aatgcgcagt
     3781 ctgtaacgtt tgtggcggat aagactacgg caactatctc caatattgag gtgattggca
     3841 actatgcgct ggcggacggc aaagccaaac aaacgtataa agttacggtg actgatgcca
     3901 ataacaattt ggtgaaagat agcgaagtga cgctgactgc cagcccggct tcgttaaacc
     3961 tggaaccgaa tggcactgcg acaacgaatg agcaagggca ggctattttc accgctacca
     4021 ctattgttgc ggcgacatac acactcaagg cgcaagtgag tcagaccaac ggtcaggtat
     4081 caacgaaaac tgccgaatct aaattcgttg cggatgataa aaacgcggta ctcaccgcat
     4141 catctgatat gcaatctctg gtggcggatg ggaaatcgac tgcgaagctg gcggtgaccc
     4201 tgatgtcggc aaataacccc gttggcggga atatgtgggt cgacattcag acgccggaag
     4261 gggtgacgga gaaggattat cagttcctgc cgtcgaaaaa tgaccatttc gtgagcggga
     4321 aaatcatacg tacatttagt accagcaagc caggcgtcta tacattcaca tttaacgccc
     4381 tgacctatgg cgggtacgaa atgaagccag tgacggtgac cattaccgcg gtggatgccg
     4441 atacggcaaa gggcgaggag gcgatgaaat aatttttaag aatatgaaaa tagcattcta
     4501 ataaacaata atggcgttga catattcaac gccattattt attgaattgc ctgacagtag
     4561 tctcgttata agttcattta gatatagcga cagctaaacc ggacgaacgg ggataaaaat
     4621 ttcgcaaaca acatcattgc tatccttttc tacgaaatct tcattgtaat aaaatcgctc
     4681 aatatcactg ccgttcactc tggctaaccc cctggcaggt aattccatca gatagaggtt
     4741 tcgcgaccat tcaggatagc gctcccactc accttcatcg cggaaagatg catacagtcc
     4801 tcggtttatt gtccactctt tcgccatctc agaatcggtt tgtttacata ccgtaccggc
     4861 aaaagtttca actgtgagca aatcgaccga ttttgttgac ggttcaaacg tggagacgaa
     4921 atagatatcc gtcttaaaat tattcataca tctggctaaa tgatgacgtc gcagtttgac
     4981 ttcttcatcc ggtgatttat tcgacacttc aatgtcatat ttaaatgagt tgccaaaaat
     5041 aggtgtctca ggaaaattga ctaatctaca ctccgtcttt tgctgttgac gtattaaaaa
     5101 agaagggaag atatttgcga gatcccaata atcacggtgg cggtattcac ggggagagca
     5161 gccgaataac tttttaaatt cgcggctgaa tgactgctgt gaatcaaaat gcaaagagag
     5221 tgcaatatca agcatagatt tcgcggtgag ccggacaaga atagcggcac ggcaaagcct
     5281 tcgtttgcga atgtattctc ccaaaggcac atgcatgaaa tttcggaaca gaagctggat
     5341 gttgcgtctg ctgtagccag atttctgtgc gatatcttcg attgaaatag ggtgctcaag
     5401 attgcactca atccactcca ggagctgctg tagaatcttc tgcctgatca tcacattctc
     5461 cattgattct tcgtcatatc gcacattttc gttatgccct gggccctaag tgtatcaaaa
     5521 acagcaagtc cgaaggcaaa agcactggta gcgtctgctt cgttgtcccc gaaaatagag
     5581 aggtttcagt cctacattat taatgaattt tttgcataag tgatatcaaa atccacatac
     5641 taatttgagg ttacgtttta acgtagactc attgttcatg tctaatggag ggactgacag
     5701 tggaattttc cgtattaagt aacaatctga aaatgccgat gatgggattt ggtgtttttc
     5761 aggttaccga taaaaacgtg tgcaaacagt cagtgttgaa cgctatccgc acgggttatc
     5821 gactcatcga cactgcagcg gtatacggta atgaagatgc cgtcggtgaa gccgttcgtg
     5881 aagctatatc tgaaggttta tgtacccgag atgagttatt tataacgtct aaattgtggg
     5941 tgcaggatat gttgaatcag gatacggcag cagcaggtat tgaagcatca ttaaaaaaat
     6001 cgggactaga atacttcgac ctctatttat tgcaccaggc tatgcgcgat tatttcagtg
     6061 cgtggcgtgc actcgaagat gcctatgaag agggcaaatt aaaagcaatt ggggtttcca
     6121 atttctatcc tcatgttctg gcgaactttt gtgaaacggt aagagttaaa ccgatggtca
     6181 accaggtcga gttgcatccc tattttgccc aaccagaggc gctggcaacc atgaagtatt
     6241 ataacgtgca gcctgaagca tgggctccgt taggtggtgg acgacataaa ccctttgaaa
     6301 ataatctgct tcagagtatt gcagatgccc atcaaaaatc gatttctcaa gtcattctgc
     6361 gttggaatat tcaacgggga gtggtcgtta ttccgaaatc gacacatcaa cagcgtatcg
     6421 aagaaaattt tgctatctgg gatttctcac tgacagagaa agaaatggca caaattagtt
     6481 cgcttgattt gggttatgtt ggggagtcgg taaaacattt taatcctgaa tttgttcgtg
     6541 gttgtcttgc tgtaaaaaat tcatgattga tattaatcat atattttacc tgagacgaca
     6601 agaatctttt aacaggggag tgatattgat cttcactctg tcatatctcc ggtaatatgg
     6661 cgtcaggctt catcatcaga aagggggcct gacgcctgaa aaagtgaaca acagacagtg
     6721 ttcggattat caatattcag tttttaacgt tgaactggat tcattactgc gttgtttaag
     6781 aatttcccgc gccgaatctg ccattatcat gacagcacct gccagcatca gagtatcttt
     6841 caataccagg cgaccagcac cggataaata agggaaacca tgatgagcgt cacccaatgc
     6901 gggtacccat gcctccgggg tggtgattaa aaatgagagt gttaccaacg gtgtggtaaa
     6961 tgccatcagc cctcccaata aacctaacca gcgattgaca ggattagcca aaaccagcag
     7021 agcaataatc acctccacga cgccaagacc gttggaaaaa ccataggtat tattggccga
     7081 ttgccatgcc cttgcttctg gtttgtattc gccttcgtga gtcagatact gtttatagtc
     7141 ttccgggtgt tcataaaaga acgacattag tggactgttt gcgacgaatg gtgtaatgct
     7201 gtctgcctcg taagggacaa actttaataa cccaatccac ataaaaacaa tcgcaatact
     7261 cagacgaatc aatgtcaggc caattttatc tccccgactt aacatgtgca ggtatttctc
     7321 cattgtaaac atccttattt atacaatact gctgttgcgg ttgaattatt atcaattcgc
     7381 atcgatgtga ctttccagga tgaagcatta tattttctgg ctatatcagc aatggcatcc
     7441 tccagaactg tcggtgatgc tgatactgac gagacgctga tatgtttcag aattatttta
     7501 tcatctgcat tggtggaaaa tgccataacg ccagataata gtgttgcaa
//
LOCUS       DAZGVQ010000215         7546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000215 DAZGVQ010000000
VERSION     DAZGVQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.217"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            121..579
                     /locus_tag="ACHLAG_004740"
     CDS             121..579
                     /locus_tag="ACHLAG_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP0657838.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIE"
     gene            849..2990
                     /locus_tag="ACHLAG_004741"
     CDS             849..2990
                     /locus_tag="ACHLAG_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP0657839.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGMVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDHSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTQDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQESSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HHENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3066..7319
                     /locus_tag="ACHLAG_004742"
     CDS             3066..7319
                     /locus_tag="ACHLAG_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element core protein"
                     /protein_id="HFP0657840.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQYSSQPLSALWQ
                     VLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAKGDVAGAVTGVTDGAGRRFHLALTTQAQRAEAFRKQRATSLSSPAGPRSA
                     SSSSAFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTASGELRAV
                     YDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRFEYG
                     QDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRR
                     TEYRLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDERGRLTAET
                     SRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEYDRY
                     GQQIAVHREEGISTYSSYNPRGQLVSQKDAQGREIRYEYSAAGDLTATVSPDGKRSTI
                     EYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGFDGR
                     TQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQWQYDDHGWLTEISH
                     LSEGHRVAVHYGYDDKGRLTGERQTVENPETGEMLWEHETGHAYSEQGLATRQEPDGL
                     PPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGAGSTAGYEQATAYTLTG
                     QLQSRHLNLPQLDCDYTWNDNGQLVRISGPQECREYRYSGTGRLTGVHTTAANLDIDI
                     PYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIPEGVI
                     RMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTGKRVWRRERDLTGW
                     MSLSRKPEETWYGWDGDRLTTVQTQQTRIQTVYQPGSFTPLLRIETENGEQAKARHRS
                     LAEVLQEDTGVTLPAELAVMLGRLERELRQGSVSEESQQWLAQCGLTAEQMAAQLEAE
                     YIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGEESAQHLQQSLRLPGQ
                     QYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGLKFHVN
                     GDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMAIYWNSRA
                     SLFCSTELNSKSQSPALGLGHEFAHAQYYLLDKENFMALLSRTDKKYENKEEARVITI
                     IESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            7331..>7546
                     /locus_tag="ACHLAG_004743"
     CDS             7331..>7546
                     /locus_tag="ACHLAG_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-h1"
                     /protein_id="HFP0657841.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEISDLYA"
BASE COUNT         1795 a         1899 c         2410 g         1442 t
ORIGIN      
        1 ttgtatgtaa aaaatactga aaaatgatgc ggttccgtga agtgagctgc atctgcctgt
       61 ctgggattaa cggcaggtca ttgtaagtag taattaacca cgttaacaag gagtttacaa
      121 atgccaacac cgtgttatat ctccatcact ggtcagaccc agggaaatat tactgcaggt
      181 gcatttaccg ctgattctgt cggcaacatc tatgtgcagg ggcacgaaga cgagatgctg
      241 gtgcaggaat tcctgcataa cgttactgtc ccgaccgatc ctcagtccgg tcagcctgcg
      301 ggtcagcgtg ctcacaagcc tttcattttt accgtggcgc tgaacaaagc ggttccgctg
      361 atgtacaacg cactggcctc cggcgaaatg ctgccaacca ctgaacttca ctggtggcgt
      421 acctctgtgg aaggcaaaca ggagcattat ttcactaccc gcctgactga ttcgacgatt
      481 gtcgatatga aactgcatat gccgcattgc caggacccgg cgaaacgcga gttcacccag
      541 ttgctcgaag tttcactggc ctaccgcaaa attgaatagg aacacgtcaa atctggcacc
      601 tccggtgccg acgactggcg cgcaccgctg gaagcataag ttaagccaac agcatccggc
      661 tatcaggccg gatgctgttt ttgtattacg ccgtagaaag aaaggttgtc agtaaggggt
      721 tacgttcacg ggagatggcg gctgccggaa acttcggcgg tagcgtgcct gaacgcaagc
      781 gtaacactgt actgtcacag ccgctttaac agcgaactgt ctgtttttcc ttaaccccgg
      841 aggttgctat gtcaaccgga ttacgtttca cgctggaagt ggacggcctg ccaccggatg
      901 cttttgcggt ggtctccttt catctgaacc agtcactctc ttcgcttttt tccctcgatc
      961 tctctctggt cagccagcag tttctctccc ttgaattcca gcagatcctc gacaaaatgg
     1021 cctacctgac gatatggcag ggcgatgacg tacagcgccg ggtgaaaggt atggtgacct
     1081 ggtttgaact gggggagaac gacaaaaacc agatgctgta cagcatgaag gtgtgcccac
     1141 cgctgtggcg cacagggctg cgccagaact tccgtatctt ccagaatgag gacatcgaaa
     1201 gcatcctcgg cacgatattg caggaaaacg gggtgaccga gtggagcccg ctgttcagcg
     1261 agccacatcc ttcccgtgag ttttgtgtcc agtatggtga gactgattac gatttcctgt
     1321 gccggatggc ggcggaggaa ggcatcttct tttatgagga gcacgcgcaa aaaagtaccg
     1381 accacagcct ggtcctgtgc gataccgtgc gttatctgcc ggagtccttt gagatcccct
     1441 ggaatccgaa cacccgtacc gaggtgagca ccctctgcat cagccagttt cgctacagcg
     1501 cacaaatccg cccttcttcc gtggtgacca aagactacac ctttaaacga cccggctggg
     1561 cagggcgttt tgatcaggaa ggccagtacc aggattacca gcgcacacag tatgaagtgt
     1621 atgactaccc cggacgtttc aagggtgccc acgggcagaa ctttgcccgc tggcagatgg
     1681 atggctggcg caacaacgca gaagtggcgc gcggaacaag ccgttcgccg gagatatggc
     1741 cgggacggcg aattgtgctg acggggcatc cgcaggcgaa cctgaaccgg gaatggcagg
     1801 tggtggcgag cgatcttcac ggcgaacagc cgcaggcggt accgggacgc aggggttcag
     1861 gcaccacgca ggataaccac tttgcggtaa tcccggcaga ccggacatgg cggccacagc
     1921 cgttgctgaa accgctggtg gacggcccgc agagcgccgt cgtgacggga ccggcaggcg
     1981 aggaaatctt ctgtgatgaa catggccgcg tgcgggtgaa atttaactgg gaccgctata
     2041 acccgtcaaa ccaggaaagt tcatgctgga tccgtgtggc acaggcgtgg gcaggcaccg
     2101 gctttggtaa cctggcgata ccgcgtgtgg gtcaggaggt cattgtggac ttcctcaacg
     2161 gcgacccgga ccagccgatt attatggggc gtacctacca ccatgaaaac cgcacacccg
     2221 gcagcctgcc ggggacgaag acgcagatga cgatccgctc gaaaacctat aagggcagcg
     2281 ggtttaatga actgaagttt gacgatgcga cagggaaaga acaggtctac atccacgcgc
     2341 agaagaacat gaacaccgag gtgctgaata accgcaccac tgatgtgata aacaaccatg
     2401 ccgaaaaaat aggtaacaac caggcgatca ccgttaccaa taaccagatc cagaacattg
     2461 gcgttaatca gatacagacg gttggtgtca accaggtgga aacggtgggc agtaaccaga
     2521 ttatcaaagt gggatcaaac caggttgaaa aggtggggat cattcgtgcg ctgacggtgg
     2581 gtgtggctta ccagacgacg gtaggcggca ttatgaatac ctcggtggcg ttgttgcagt
     2641 cctcacaggt agggctgcat aaatcactga tggtgggaat gggctacagc gtcaatgtgg
     2701 ggaataacgt caccttctcg gtgggcaaga caatgaagga aaacaccgga caaacagcag
     2761 tttattctgc cggtgagcat cttgaactct gctgtggtaa ggcaaggctg gtgctgacga
     2821 aggacggaag catatttctc aatggtacgc acattcatct ggaaggggag tcggatgtga
     2881 acggtgatgc gccagtgatt aactggaact gtggtgccac acaacctgta ccggatgcgc
     2941 ctgtgccgaa agatttaccc cccggaatgc cggatatgcg gcaattttga gtcaggtgca
     3001 aaaaattaaa acggaactgg tgcagtgacc agataaacca tattaaaagc agaaggaatt
     3061 gattcatggg cggaaaaccg gcggcgcggc agggtgatat gacccgcaag ggactggata
     3121 ttgtgcaggg ttcagcgggg gtgctgatag gtgcgccgac gggagtggcc tgttcggtat
     3181 gtccgggagg gattacctat gctaacccgg tgaacccgct gctgggtgcg aaggtgctgc
     3241 cgggcgagac ggaccttgcg ctgcccggcc cgctgccgtt tattctttcc cgcgcctaca
     3301 gcagctaccg gaccagaaca cccgcgccgg tgggggtgtt tggtcccggc tggaaagcgc
     3361 ccttcgatat ccgcttacag atacgcgatg aaggcctgat actcaacgac agcggcggca
     3421 ggagcattca ctttgagccg ctgtttcccg gcgagataag ctacagccgc agcgagtcac
     3481 tctggctggc gcggggcgga gtagcggcgc agtacagttc gcagccgcta agcgcgctct
     3541 ggcaggtgct gccggaagat gttcgcctga gtccgcatgt gtacctggcg acaaacagcc
     3601 ttcaggggcc gtggtggata ctcagctggc cggagcgggt gccgggggcg gacgaggtgc
     3661 tgccgccgga gccgcccgca taccgggtgc tgacgggcgt ggtggacggc ttcgggcgga
     3721 cgctggcctt tcaccgggca gctaagggtg acgtggcggg cgcggtgacg ggggtgacgg
     3781 acggcgcggg ccgccgtttt cacctggcgc tgaccacgca ggcgcagcgg gcggaagcct
     3841 tccgtaaaca gcgcgccacg tctttatctt cccctgccgg tccccgttct gcttcctctt
     3901 cttctgcttt ccccgacacg ctgcccgccg gtacagaata cggtgccgat aacggtatcc
     3961 ggctggaggc ggtgtggctg acacacgacc cggcataccc ggatgaacag ccgactgcgc
     4021 cgctggcgcg ctacacgtac acggccagcg gagaactgcg ggcggtgtat gaccgcagcg
     4081 gaacgcaggt gcgcgggttt acttatgatg cggagcacgc cgggcggatg gtggcgcacc
     4141 attatgcagg gagaccggag agccgctacc ggtatgatga taccgggcgg gtgacggagc
     4201 aggtcaaccc ggaggggctg gactatcgct ttgagtacgg gcaggaccgt gtgaccatca
     4261 cggacagcct gaaccggcgg gaggtgctgt acacggaggg cgaaggcggc ctgaagcggg
     4321 tggtgaagaa ggagcatgcc gacgggagca tcacccgcag cgagtatgat gaggcgggga
     4381 ggctgaaggc gcagacggat gcggcggggc ggcggacgga gtaccggctg catatggcgt
     4441 cgggcgcggt gacagcggtg acggggccgg acggcaggac ggtgcggtat ggctataaca
     4501 gccagcggca ggtgacgtca gtgacgtacc cggacgggct tcgcagcagc cgggagtatg
     4561 atgagagggg aaggctgacg gcggagacct cgcgcagcgg tgagacaacg cggtacagct
     4621 atgatgaccc ggcgagtgag ctgccgacag ggatacagga cgcgacgggc agtacaaaac
     4681 agatggcatg gagccgttac ggtcaactgc tggccttcac ggactgctcg gggtacacga
     4741 cgcggtatga gtacgaccgg tacggtcaac aaatcgccgt tcaccgggag gaaggcatca
     4801 gcacttacag cagttataac ccgcgtgggc aactggtcag tcagaaggat gcgcaggggc
     4861 gtgaaatccg ttatgagtac agcgccgcag gcgacctgac agcaacggta tcaccggacg
     4921 gtaaacgcag caccatcgaa tatgataaac gcgggcgtcc ggtgagtgtg acggaaggcg
     4981 gtctgacgcg cagcatgggg tatgacgctg ccgggcgcat cactgtgctg accaacgaga
     5041 acggcagcca gtccacgttc cggtatgacc cggtcgacag gctgacggag cagcgcggct
     5101 ttgacggccg gacgcaacgt taccagtatg acctgaccgg aaaactcacg cagagtgaag
     5161 atgaggggct tatcaccctc tggcattacg atgcgtcgga ccgtatcacc cggcgcacgg
     5221 tgaacggtga accggcggag cagtggcagt atgacgacca cggctggctg acagaaatca
     5281 gccacctgag tgaaggccac cgtgtggcgg tccactacgg ctatgacgat aaaggccgtc
     5341 tgacgggtga acggcagacg gtggagaacc cggagacagg ggagatgctg tgggagcatg
     5401 agacggggca tgcgtacagc gaacaggggc tggcgacccg tcaggagccg gacggtctgc
     5461 cgccggtgga gtggctgacg tatggcagcg gttatcttgc ggggatgaag ctgggcggaa
     5521 cgccgctggt cgagtacacg cgggaccggc tgcaccgcga gacggcccgt agcttcggcg
     5581 gggcgggcag tacagcgggg tacgaacagg ccacggcgta caccctcaca gggcagctac
     5641 agagcaggca cctgaacctt ccgcagcttg actgtgacta tacgtggaac gataacggac
     5701 aactggtgcg catcagcggc ccgcaggagt gccgggagta ccgttacagc ggcacgggaa
     5761 ggctgacggg cgtccacact acggcagcga acctggatat cgatatcccg tatgcgacgg
     5821 acccggcagg aaaccggctg ccggacccgg aactgcaccc ggacagcacc ctcacggcgt
     5881 ggccggataa ccgcatcgcg gaagatgcgc actatgtcta ccgctacgat gaatacggca
     5941 ggctggcgga gaagacggac cgcatcccgg aaggggtgat acggatgcac gacgagcgga
     6001 cccaccacta ccactacgac agccagcacc gtctggtgtt ctacacgcgg atacagcacg
     6061 gtgagccgca ggtggagagc cgctacctct atgacccgct gggccgccgg acgggaaaac
     6121 gggtgtggcg gcgggagcgt gacctgacgg ggtggatgtc gctgtcgcgt aaaccggagg
     6181 agacctggta cgggtgggac ggtgacaggc tgaccactgt acagacccaa cagacaagaa
     6241 tccagacggt ataccagccg ggaagcttca cgccgctcct gagaatcgaa acagagaatg
     6301 gtgaacaggc gaaggcgcgg caccgtagtc tggcggaggt gttgcaggag gacacgggtg
     6361 tgacgctacc ggcggagctg gcggtgatgc tggggaggct ggagcgggaa ctgcggcagg
     6421 gcagcgtgag tgaagaaagc cagcagtggc ttgcgcagtg cgggctgacg gcggaacaga
     6481 tggccgcgca actggaggcg gaatacatcc cggagaggaa acttcatctt taccactgtg
     6541 accaccgtgg gctgccgctg gcgcttatca gcccggaagg ggaaacggcg tggcaggggg
     6601 agtatgacga gtggggaaac ctgctgggcg aggaaagcgc gcagcacctt cagcagtcac
     6661 tccgtctgcc gggacagcag tatgatgagg agtcggggct gtactacaac cgcaaccggt
     6721 actatgatcc gttgcagggg agatatatca cccaggaccc gatagggctg agaggtgaat
     6781 ggaatctgta taaatatcca ttaaaccctg ttcggtttat agattctttg ggtttaaaat
     6841 tccatgttaa cggtgatcca agtgatttta atcaggctgt tgagtattta aaacaagatt
     6901 cacaaatgaa agaaactata gattttttat caagctctga agaaactata aatatagagt
     6961 atattgaagg aaccaatgtt agatttaatt cgaataatat ggcgatatat tggaatagta
     7021 gagcatcttt attttgttca actgaattaa actcgaaatc acaatcgcct gcattaggtt
     7081 tgggacatga gtttgctcat gcgcagtatt accttcttga taaggaaaat ttcatggctc
     7141 ttttaagccg aacagataag aaatatgaga ataaagaaga ggcaagggtt attacaataa
     7201 ttgaatcgcg tgcggcgaaa actttaggtg agtgtaccag aggagctcat agtggactgc
     7261 ctttttatcg ggtggatgga ccgttgcaga cgatgaaaat aacgggtacg ccagaatagt
     7321 gagatttttt atgatgaaat atttgatggt tttattgtct ctattttctg gttcggtact
     7381 ggggatgggg cgagtgaatg aattgtgtgg aatagatagc gtgaaaacga tagagataat
     7441 taatcttcca tcgtatgtta ccacgttagt tccgcttagc aaggaaggtc ttaatgaaat
     7501 atacagatat aaagtggtgg tgaatgaaat tagcgatctt tatgct
//
LOCUS       DAZGVQ010000216         7513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000216 DAZGVQ010000000
VERSION     DAZGVQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.218"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..844
                     /gene="gatY"
                     /locus_tag="ACHLAG_004744"
     CDS             <1..844
                     /gene="gatY"
                     /locus_tag="ACHLAG_004744"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFP0657842.1"
                     /translation="STKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAPVIIA
                     GTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVMIDAS
                     HLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEYDVQVNEADAFYTNPAQAREFA
                     EATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDIQQTI
                     KLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIADCGC
                     EGRA"
     gene            873..2135
                     /gene="gatZ"
                     /locus_tag="ACHLAG_004745"
     CDS             873..2135
                     /gene="gatZ"
                     /locus_tag="ACHLAG_004745"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFP0657843.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDTPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            2145..2597
                     /gene="gatA"
                     /locus_tag="ACHLAG_004746"
     CDS             2145..2597
                     /gene="gatA"
                     /locus_tag="ACHLAG_004746"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFP0657844.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            2628..2912
                     /gene="gatB"
                     /locus_tag="ACHLAG_004747"
     CDS             2628..2912
                     /gene="gatB"
                     /locus_tag="ACHLAG_004747"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFP0657845.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            2916..4271
                     /locus_tag="ACHLAG_004748"
     CDS             2916..4271
                     /locus_tag="ACHLAG_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFP0657846.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            4319..5359
                     /gene="gatD"
                     /locus_tag="ACHLAG_004749"
     CDS             4319..5359
                     /gene="gatD"
                     /locus_tag="ACHLAG_004749"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP0657847.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            5465..6238
                     /locus_tag="ACHLAG_004750"
     CDS             5465..6238
                     /locus_tag="ACHLAG_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP0657848.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(6320..7219)
                     /gene="yegS"
                     /locus_tag="ACHLAG_004751"
     CDS             complement(6320..7219)
                     /gene="yegS"
                     /locus_tag="ACHLAG_004751"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFP0657849.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         1890 a         1690 c         1951 g         1982 t
ORIGIN      
        1 atcgacaaag cagatgctga acaacgcaca gcgcggcggt tatgcggttc cggcattcaa
       61 tattcacaat ctcgaaacga tgcaagtggt ggtggaaacc gctgccaacc tgcatgcgcc
      121 ggtcatcatc gccggaacgc ctggcacatt tactcatgct ggtacagaaa atctgttggc
      181 gctggtcagc gcgatggcga agcaatatca ccatccactg gcaattcatc tcgaccatca
      241 cacgaaattt gacgatatcg ctcagaaggt tcgttctggc gtgcgctcag tcatgattga
      301 cgcctcgcat ttgccttttg cgcaaaatat ttcacgggtc aaagaggtgg tggatttttg
      361 ccatcgcttt gatgtcagcg tcgaagcgga gctggggcaa cttggcggcc aggaatatga
      421 tgtgcaagtc aatgaagccg atgcttttta taccaacccc gctcaggcgc gtgaatttgc
      481 cgaggcaacc ggaattgatt ccctggcggt cgccatcggc acggctcatg ggatgtatgc
      541 cagcgcaccg gcgcttgatt tttctcgact ggagaacatt cgccagtggg tgaacttacc
      601 gctggtgctg catggcgcgt cagggttatc gactaaggat attcagcaaa ccatcaaact
      661 ggggatatgc aaaatcaacg ttgcaacgga gctgaaaaat gccttctcgc aggcgttaaa
      721 aaattacctg accgagcacc ctgaagcgac cgatccccgg gattatttgc agtcggctaa
      781 atccgcaatg cgcgatgtgg tgagcaaagt gattgccgat tgtggctgcg agggcagggc
      841 ataacgcacc tgccatttaa caaggaaaaa acatgaaaac gttaattgcc cggcataaag
      901 ctggtgaaca tatcggcata tgttcagtct gttctgccca tccgttggtt atcgaagcgg
      961 cgctggcatt tgatcgcaac agcacgcgca aagtgctgat tgaagcaacg tcaaaccagg
     1021 tcaatcaatt tggcggttat accggaatga caccggcaga ctttcgcgaa tttgttttta
     1081 cgattgccga taaagttggg tttgcacgcg aacgcattat tctcggcggc gatcatctgg
     1141 ggccaaactg ctggcagcaa gaaaatgcgg atgcggcgat ggaaaaatcc gtcgagctgg
     1201 taaaagcata tgttcgtgct ggcttcagta aaattcatct tgatgcgtca atgtcctgcg
     1261 cgggggatcc cataccgtta gcaccagaaa cggttgcgga acgagctgct gtgctttgct
     1321 ttgctgcgga aagtgtggcg acagattgcc agcgtgagca actgagctat gtcattggca
     1381 ccgaagttcc ggttccgggc ggtgaggcca gcgccattca gtcagtacac atcacccatg
     1441 ttgaagatgc cgccaatact ttacgtacgc atcaaaaggc ctttattgcc cgtgggctga
     1501 cagaggcgtt aacacgtgtg attgccatcg tggtgcagcc gggtgtggaa tttgatcaca
     1561 gcaatattat ccattatcag ccgcaggaag cgcaggcgct ggcgcaatgg atagaaaaca
     1621 cccgaatggt ttatgaagca cattctaccg attaccagac ccggacggct tattgggaat
     1681 tagtccgcga tcactttgca atattgaaag tcggtcccgc attaaccttt gctttacgcg
     1741 aggcgatatt tgcactggca caaattgagc aggaacttat cgcccccgaa aatcgcagcg
     1801 gttgcctggc ggtaattgaa gaagtgatgc tcgacgaacc gcaatactgg aaaaaatatt
     1861 atcgtacggg ttttaacgat tcattactgg atattcgtta cagcctgtcg gatcgtattc
     1921 gttattactg gccgcatagt cggattaaaa atagcgtcga aacgatgatg gtgaatcttg
     1981 aaggcgtgga caccccactg ggcatgatta gtcagtatct tcccaaacaa tttgaacgca
     2041 ttcagtccgg ggaattatca gcaataccgc atcagctgat tatggataaa atttatgatg
     2101 ttttgcgcgc ctatcgctac ggctgtgcgg aataaggacg gtatatgact aacctgtttg
     2161 ttcgtagcgg aatttctttt gtcgatcgta gcgaagtttt aacccatatc ggtaatgaga
     2221 tgctcgccaa aggtgtggtt catgatacct ggccacaggc attaattgcc agagaagcag
     2281 aattccctac cgggataatg cttgagcagc acgccattgc aataccgcat tgtgaggcga
     2341 ttcatgctaa gtcgtcagcc atttatctgt taaggccaac aaataaagtt cattttcagc
     2401 aagcggatga tgataacgac gtggaggtat cgttggttat tgcgttgatt gtggaaaatc
     2461 cgcagcagca attgaaactt ttacgctgtt tatttggcaa gttacaacag cccgatatcg
     2521 tcgagacact aatcactctt cctgaaaccc agttaaagga atacttcaca aagtatgttt
     2581 tagattcaga cgaataaatc cctctgtaac aataaaaagg actatttatg aaacgcaaga
     2641 ttattgtcgc ttgcggaggc gcggttgcga cctctacgat ggcggcggaa gaaattaaag
     2701 agttgtgtca gaatcataat attcctgttg aattaatcca gtgtcgggtt aatgaaatag
     2761 aaacctatat ggatggtgtg catttgatat gcaccactgc caaagtggat cgtagttttg
     2821 gcgatattcc gttagttcac ggcatgcctt ttatttctgg tgtcggtatc gaagcattac
     2881 aaaataaaat tctgactatc ttacaggggt gacctatgtt ttcagaagtc atgcgttata
     2941 ttctcgacct cggccctaca gtgatgctgc cggttgtcat cattattttt tctaaaatat
     3001 taggcatgaa ggcaggcgat tgctttaaag cgggtctgca tatcgggatt ggctttgttg
     3061 gcattggcct tgtgattggc ttaatgctgg attccattgg tccggcggcg aaagcgatgg
     3121 cggaaaattt cgacctgaat ctgcatgtgg tcgatgtcgg ctggccgggc tcttcaccaa
     3181 tgacctgggc gtcgcaaatt gcgctggtgg cgattccgat tgcgatattg gttaacgtgg
     3241 cgatgttact gacccgtatg acgcgggtgg taaatgttga tatctggaat atctggcata
     3301 tgaccttcac cggcgcgttg ctgcacctgg caaccggttc atggatgata gggatggcag
     3361 gtgtggtaat tcacgcggcg tttgtttata agctcggcga ctggtttgcc cgcgataccc
     3421 gaaatttctt tgagctggaa ggtattgcta ttccgcacgg tacgtcggcg tatatggggc
     3481 cgattgcggt gctggtcgat gctatcatcg agaaaatccc aggcgttaac cgaattaaat
     3541 ttagcgccga cgatattcag cgcaaatttg gcccttttgg cgaacctgtc accgtgggtt
     3601 ttgtgatggg gctgattatc ggcatcctcg cgggttacga tgtcaaaggt gtattgcagc
     3661 tggcggtaaa aacggcggca gtgatgctgc tgatgccacg ggtgattaaa cccatcatgg
     3721 atggtttaac gcccatcgct aagcaggccc gtagtcgttt acaggcgaag ttcggcggtc
     3781 aggagttcct gattggcctg gatcccgcat tactgctggg gcatacggcg gtggtatcgg
     3841 caagcctgat ttttatccca ctcaccattt taattgctgt ttgtgtgccg ggtaatcagg
     3901 tgctgccgtt tggggatctt gccactatcg gcttctttgt ggcgatggcg gtcgcggtgc
     3961 atcgtggaaa tctgttccgc accttaatct cgggtgtcat cattatgagc atcaccctgt
     4021 ggatcgcgac gcaaactatt ggtttgcaca cccaactggc ggctaatgct ggggcgttaa
     4081 aagccggggg tatggtggct tcaatggatc agggcggttc tcccattacc tggttactga
     4141 ttcaggtttt ctccccgcaa aatattcccg gtttcattat tatcggtgca atttatctga
     4201 ccggtatttt catgacctgg cgtagagcgc gtggctttat taaacaagag aaagtcgttc
     4261 tcgcagaata atttttacct gagggggagg ttatccccct taacaatcag gagtttttat
     4321 gaaatcagtg gtgaatgata ctgatggtat cgtgcgcgtt gcagaaagcg tcattcctga
     4381 aattaaacat caggatgagg tgcgggtaaa aattgccagc tcgggcttat gtggttccga
     4441 tttacccagg atatttaaaa atggtgcaca ttattatcca ataacgttag gccatgaatt
     4501 tagcggctat attgatgcgg tgggatccgg tgttgatgat ttacaccctg gcgatgcggt
     4561 tgcctgtgtg ccgttattac cctgttttac ttgtccagag tgtctgaaag ggttttattc
     4621 ccagtgcgca aaatatgatt ttattggctc gcggcgtgat ggtggatttg ctgaatatat
     4681 tgtcgttaag cgaaaaaatg tctttgctct acccacggat atgcctattg aggatggggc
     4741 ttttattgag ccgattaccg ttggtctgca tgcttttcat ttagcgcaag gttgtgagaa
     4801 taaaaacgtt attattattg gtgccggaac cattggcctg ctggctattc agtgcgctgt
     4861 cgcgctggga gcaaagagtg tgacggctat cgacattagc tcagaaaaac tggcactggc
     4921 aaaatctttc ggtgcgatgc aaacatttaa cagtagcgaa atgagcgcgc cgcaaatgca
     4981 gagcgtttta cgcgaactgc gttttaatca gcttatcctc gagacggctg gcgtaccgca
     5041 aactgtcgaa ctggcggtag agattgccgg tcctcatgcc caactggcgc tggtgggcac
     5101 gttgcatcag gatctgcatt taacatcggc aacgtttggc aaaatattgc gtaaagagct
     5161 gacggttatc ggcagttgga tgaactattc cagcccttgg ccggggcagg agtgggaaac
     5221 ggcgagccgg ttgctgacag aacgtaagtt aagcctggag ccattaatcg ctcaccgtgg
     5281 aagctttgaa agcttcaccc aggcggtgcg tgacatcgct cgtaatgcca tgccgggcaa
     5341 agtgttgctc attccatgaa accgcgggcc agcgtgatgc tggcccggta ttgtgcaaaa
     5401 cagatcattc accaatggtc ccccttcgtt tacactagcc acaattgaat atgggtaaat
     5461 gacgatgaat tcattcgagc gaaggaataa gatcatccaa ttagtgaatg aacagggaac
     5521 cgtgcttgtt caggatctgg cgggagtatt tgctgcctcg gaagcgacaa tccgtgccga
     5581 tttgcgcttt ctcgaacaaa aaggcgtggt tacgcgcttt catggcggtg cggcgaaaat
     5641 aatgtccggt aatagtgaaa ccgagaccca ggaagtcggg tttaaagagc gatttcagct
     5701 cgccagcgcg cccaaaaaca gaatagcgca ggcggcagtc aaaatgatcc atgaagggat
     5761 gaccgttatt ctcgacagcg gaagtaccac aatgcttatc gccgaaggat taatgaccgc
     5821 taaaaatatc acggtgatta ccaacagtct cccggctgcg tttgcccttt ccgaaaataa
     5881 agacattact ctggtcgtct gcggtggcac ggtccgccat aaaacgcgat ctatgcatgg
     5941 atctattgct gaacgttccc tgcaggatat taatgccgat ttaatgtttg tcggtgctga
     6001 tggtattgat gcggttaatg gtattacgac ctttaatgaa ggttattcga ttagcggtgc
     6061 gatggtcacc gccgctaata aagtgattgc cgttctcgat tcatcgaaat ttaaccgtcg
     6121 cggttttaat caggtactgc caatcgaaaa aattgacatt attattaccg atgatgctgt
     6181 atcagaggtg gataaactgg cattgcagaa gacacgggta aaattaatta cggtgtagta
     6241 ttgttctgta tgccggataa ggtatttaca ccgcatccgg catttcggat tctccctggt
     6301 tcaagagaga atagtttgat taacgcaata atggacaatc cggtggtaat cgacaacgca
     6361 acgccgccgg aagtatttca atatggaagt tttgcccact caacggttcg ccatcaagat
     6421 taaaagtgat ttcgtgtggg gcttgtatat caaaccacga cgaagcgcct tcgataatat
     6481 tcgggttatc ttcgtcagat tttaaggttg atacgagagc cggaagaatt tcatcgccgg
     6541 taaaaatgcg cagttgcagc aagccatcgt taattaacgc gttcgggcac aattgttgac
     6601 cgccaccggc ctgacgcccg ttaccaatac caatgaccag ggcgtcacct tgccagtgaa
     6661 agttttcacc gcggatttca caacggtccg gttgcagagt atccatgcgc attaagccat
     6721 gaatgatgta agagacgcca cccagcgcgg cttttaattt ttccggcgtt tctgtggtaa
     6781 tacgcgtccc aaatccgcct gtcgccatat taataaaaca ggtttgtttg ttgacctgcg
     6841 ccatatctat cgcaatggcg ttaccggcaa ttgccagttt cagcgcctta tccagtgcct
     6901 caggaatccc tacactggtg gcaaaatcat tggcggttcc taatggcaaa attcccagcg
     6961 cgggtatgtc atccccctca cactgaatca acgccgtaga aacttcatta atggtgccat
     7021 cgccaccacc ggcaatcacc gttgcgacgc ccaacttccg ggcctcctct acatatcgtg
     7081 cggcatcgcc tttctcccag gtgacccgca catggatcgt cattccttcc tcacgcaaca
     7141 gcataattgc ttcgcgcaag ggtagattgt cagtactttt gccattaaga atcagtaagc
     7201 tggcgggaaa ttctgccatg atcgtatgtg cctttatgat tggtctgtag atagtgtaga
     7261 gcagaaaaca aaaggtggag tcagaacagg atgaaagtcg gaggataaaa agtcagttca
     7321 agtatggagg attcagtggt taccaccagg agcttgcaat ggggacagcg aatactaacg
     7381 gctgccaccg ataaatttca aaaaagagca tatacctaat attcaactaa acagtggcat
     7441 cttcaatata atatattaaa gcccccatgg agttaccctg aagggcctca atgtccgtaa
     7501 ttcctactta tgt
//
LOCUS       DAZGVQ010000217         7386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000217 DAZGVQ010000000
VERSION     DAZGVQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.219"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..592)
                     /locus_tag="ACHLAG_004752"
     CDS             complement(<1..592)
                     /locus_tag="ACHLAG_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFP0657850.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWA"
     gene            complement(605..2386)
                     /gene="gcl"
                     /locus_tag="ACHLAG_004753"
                     /pseudo
     CDS             complement(605..2386)
                     /gene="gcl"
                     /locus_tag="ACHLAG_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
     gene            complement(2476..3291)
                     /gene="allR"
                     /locus_tag="ACHLAG_004754"
     CDS             complement(2476..3291)
                     /gene="allR"
                     /locus_tag="ACHLAG_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFP0657851.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCL
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(3369..3851)
                     /gene="allA"
                     /locus_tag="ACHLAG_004755"
     CDS             complement(3369..3851)
                     /gene="allA"
                     /locus_tag="ACHLAG_004755"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFP0657852.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            4081..5007
                     /gene="allS"
                     /locus_tag="ACHLAG_004756"
                     /pseudo
     CDS             4081..5007
                     /gene="allS"
                     /locus_tag="ACHLAG_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
     gene            5076..6170
                     /gene="mnmH"
                     /locus_tag="ACHLAG_004757"
     CDS             5076..6170
                     /gene="mnmH"
                     /locus_tag="ACHLAG_004757"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFP0657853.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQAIIELSQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            6286..6651
                     /gene="ylbG"
                     /locus_tag="ACHLAG_004758"
                     /pseudo
     CDS             6286..6651
                     /gene="ylbG"
                     /locus_tag="ACHLAG_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(7172..>7386)
                     /locus_tag="ACHLAG_004759"
     CDS             complement(7172..>7386)
                     /locus_tag="ACHLAG_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657854.1"
                     /translation="RFKKTLTVRAREQVAKIVSKNDPDTKKVWCKYGKIPGQGDGVNL
                     FFVGEINVTHYFITNIGAGLPDACAE"
BASE COUNT         1884 a         1929 c         1763 g         1810 t
ORIGIN      
        1 cagcccactg agtcatggtg ttggttaatt caccttccat ccgctgcatg tgataaatgt
       61 catactgaat tttcaaattg cagcaaccaa catcatcaat caatttcagc gcctgccgag
      121 ttccggtgag atggaaacca ggaatatcaa aatggttaat aggttcaatc agtaataaaa
      181 tatcttcttt catcagcata ttcgcggcat aacgcaggtt ttctacaagc gttgcgtgaa
      241 tctgttcact gctgaaacca gccggcgttt taccgaccag acagttaatt tttttattac
      301 ccagcgcacg ggcataacga atcgctgctg ctacgccatc ccgaaactct tcttcacggc
      361 caggaataca ggcaataccg cgctcccccg ccgcccagtc acccgccggt aaattgtgca
      421 gcgtatgttc gagtttatta ctcgccagca cctgttttaa ttcttcaatg tcgtagtcat
      481 aaggaaacat aaattcaacg ccgcgaaaac cacactgcgc agctttctca aaacgggcga
      541 gaaaatcata ttctccaaat aacatcgata aattagcaga gaaacgtaac attatttatc
      601 tcccttattc atagtgcatg aagcaggttt cagtcggtgc gtccgctgcg ttatcggcga
      661 tatcttcaaa ttccatgacg ttatccagtt cgctgcccat cgaaatattg gtcacacgct
      721 cgagaataac ttccacgact accggtaccc gatattgcgc cattaaggct ttcgcctgtt
      781 caaaggctgg cgcaatatct tccggtttga agacgcgaat cgctttacaa cctaaacctt
      841 ccgctacttt tacgtggtca acagcgtagc cattcacttc actggagttg atattctcga
      901 aagcgagttg cacgcagtag tccatatcaa acgcgcgctg cgactggcga atcagcccca
      961 gataagcgtt attgaccagt acatggatgt acggaatgtt gaactgtgcg ccaacggcca
     1021 attcttcaat caggaactgg aagtcgaagt caccggaaat cgccaccact ttgcgctctg
     1081 gatcagcggc acaaaccccc agcgccgccg gaatggtcca gcccagcggg cctgcctgcc
     1141 cacagttgat ccagtggcgg tctttaaaga catgcagcat ttgcgcagcg gcaatttgcg
     1201 acagaccaat ggtggtgaca tagcaaacat cgcgaccaaa ggctttgttc atctcttcat
     1261 acacgcgctg cggtttcacc ggcacgttgt cgaaatgggt tttgcgcagc aaagtgcgtt
     1321 tgcgctgctg gcagtcggcg acccattctt tacgacacgg cagacgaccc gctttttgca
     1381 tctcctgcgc cacttcaacc agcagtgtca gcgccgcttt agcatcagag acaatgccga
     1441 gatccggaca cagcacgcga ccaatttgcg tcggctcaat atcgatatga acgattttgc
     1501 gcccttcggt gtatttctct accgaaccgg tatgacggtt agcaaaacgg ttaccgatac
     1561 caaacaccat gtcggacgcc agcaacgttg cgttaccgta acgatgcgcg gtttgcagac
     1621 ccaccatccc ggccatcagt tcatgatcgt ccgggataca gccccagccc atcagcgttg
     1681 ggatcaccgg aacgctggtc agttcagcaa actgttgtaa cagtgcagct gcgtcagcat
     1741 taattacccc gcccccggca acaatcaccg gacgttcggc ctagattaac atttctacag
     1801 ctttttcgat ctgcatacgg ctggcagcag gtttgtagac cggcagcggt tcgtacatgt
     1861 caggatcaaa ctcgatttcc gcaacctgaa cgtcgaacgg taaatccacc agtaccggac
     1921 ccggacgacc agaacgcatc aggtgaaatg cctgttgcag cacacgaggc accagcgccg
     1981 cttcacgtac tgtaaccgcc attttgctga ccggcttggc gatagcttcg atatcaacag
     2041 cctggaaatc ttctttatgc agacgggcgc gcggagcctg gccggtaata cacagaatag
     2101 gaatggaatc agcagaagca gaatagagcg cggtgatcat gtccgtgccc gcaggaccgg
     2161 aagtccccag acatacgccg atattccctg ccgttgcgcg ggtataacct tccgccatgt
     2221 gcgaagcacc ttccacatga cgcgccagaa tgtgacgaat accgccgtgc ttacgcatcg
     2281 ctgagtagaa cggatttatt gcagctcccg gaacgccgaa ggcggtggtg ataccttctt
     2341 tctccagcac atacattgcc gcgtcaacgg ctctcatttt tgccatttta ttcctacctc
     2401 tattaattgg aaaatttttc caactttcaa attttgtcaa aaaaaaccgc tccgcagagc
     2461 gggatgcgac agacattatg gatgtgcttt cagtcccaac gccgtgctga tatcgcgggc
     2521 ggtgtctctg accagctcac cctgactgac aaaacgatct tctgtcagtc ttgatgaagg
     2581 cccggagatg gagatagcgg caacaacact acctacatca tcgtaaattg ctgaagctaa
     2641 gcaattcaga cctacaacat gctcttcttt atctacggta tagcccagtt cacgcgcttg
     2701 ttccaggtcc ttcagcaagg tgggcatatc cacaagcgta gttggcgtaa actgctgcaa
     2761 accggtttgc agaatgatgc tcatcaactc ctcttccgcc agcggataaa gcagcgcttt
     2821 gcccgcaccg gaagcatgca gcggcagacg actgcccagt ggcgcacaca tcctgaccat
     2881 tgatttacac tctaactgac caattaatac tgcttcattg ccgttacgga ttgcgacatt
     2941 gaccgtttcg ccggaaagta acatcaggcg gcgcataaac ggcccggcga cggagaggac
     3001 atcgcggtta tggatgtacg ccgcaccgac gttaaagaca cctaatccta tatgccacca
     3061 gcctaactga ctgtcctgat agacaaaatc cgctgcctgt aaaaccttca gcaagcgaaa
     3121 ggtcgtggag agcggcaaat ccagattgag agaaatatcg ctaaccgacg agcttccccc
     3181 acttttttcc agatattgca gaatcgcaat tccccgctct aacgcctgtg cgcccttctg
     3241 tgccacaggc tccgcctgtc ctggcctgcc gcgccgtcta acttccgtca tacctacgtt
     3301 aactccgacc tacaagtgag cataaacaat gaacaattag tttatcttat gcaagtcggt
     3361 tgcaggcgtc acgcaaaaca gagttcctgt tcaggaatac tttcaacatc acagttgtca
     3421 ctgccgccgc gatcgatggt cagaaaatcg gtgacgcgct gccaggcgaa aagtgggtga
     3481 tgccagacgt tacgatggta attcactccc tgttcgccgt tggtgataaa cgcccgcagc
     3541 gttgacaggt ctggtttgtc gtcgcctaac gccacgacca ccacaaacac ttcacctttc
     3601 atcgggataa aggcctgagt gcccagcgga tgacgttcga gttcgtgaat ggtcagcggc
     3661 agattcgccg gttgcgcgcg gttaatgctg ataagcgtac ggtcttgctc aagaatctca
     3721 accagcgcca aatcgtggta acgctccacc aggccattgt taatatggaa aaaatcccgt
     3781 tgctgagttt cgattacgtc gccataagca ctaaaggctt cctgacttaa cggtaatacc
     3841 tgaagtttca tctttttgtc cttgtaacat aattccgttc aaccatcgcc gaccggtcag
     3901 cttggtttca ataacaggca atctaaaacc ttaagagaac aacatcaata caaaagttgg
     3961 aatatttttc caaaactaga tttagtccac aaattgaaca gggttatcat gtaaaaatac
     4021 tgataaatca gaggataaga aaatcacgca acttgcggat ttaaaagggg atacctgaag
     4081 atgttcgatc cagaaacctt gcggactttc attgcggttg ctgaaacagg aagtttttca
     4141 aaagcggcag aacgattatg taaaaccacg gcgacgatca gttatcgcat taaacttctg
     4201 gaagagaata ccggagtagc gctgtttttc cgcacgactc gcagcgtgac gttgacagcg
     4261 gctggcgagc atctactttc ccaggccaga gactggctga gctggctgga aagtatgcca
     4321 agcgagctgc aacaggtgaa tgatggcgtg gaacgctagg tgaatattgt catcaacaac
     4381 ctgctctacc acccccaggc cgtcgcccag ttgctggcgt ggctgaatga acgttacccc
     4441 tttacccagt ttcacatctc ccgacaaatc tatatgggcg tctgggattc gctattatac
     4501 gaaggttttt cactggctat cggcgtcacg ggaaccgagg cgctggcaaa tacctttagt
     4561 cttgatccct taggatcggt gcaatggcgc tttgtcatgg cggcggatca tccgctggcg
     4621 aacgttgaag agccgctaac agaagcgcag ttgcggcgct ttccggcggt caatattgaa
     4681 gacagcgccc gcaccttaac caaacgcgtc gcctggcgat tgccagggca aaaagagatt
     4741 attgttcctg atatggaaac gaaaatcgcc gcccatcttg cgggcgttgg cattggtttt
     4801 ttgccaaaat cgctttgcca gtcaatgatc gataatcaac aactggttag ccgggtaatc
     4861 ccaacgatgc gccctccttc gccattgagt ctggcatggc gcaaatttgg cagcggcaaa
     4921 gcggtagaag atattgtgac cttgtttacc cagcgcaggc cggaaatcag cggattttta
     4981 gaaattttcg gcaacccacg cagttaaaaa tcaagtttcc ttgcgcgttg gcgcgtaaaa
     5041 tagcgcgcaa gctgatttga ggttcacttt taactatgca agagagacac acggaacagg
     5101 actatcgtgc cctgctgatt gctgatacgc ccattattga tgttcgcgcc cctatcgagt
     5161 ttgagcaagg cgcaatgccc gccgctatca atctgccgtt aatgaataac gatgaacgcg
     5221 ccgccgttgg cacctgctat aaacagcaag gctcagacgc agcgctggcg ctggggcata
     5281 aactggtggc gggtgaaatt cgtcagcagc gcatggacgc ctggcgggca gcgtgcctgc
     5341 aaaatccgca aggtattctc tgctgcgccc gtggcggtca acgttcacat attgtgcaac
     5401 gctggctgca tgaagcgggg attgattatc cgctggtgga aggcggttat aaggcactgc
     5461 gccagaccgc gattcaggcg atcattgaac tgtcacaaaa accgatagtg ctgattggcg
     5521 gttgtaccgg cagcggtaaa acgctattag tgcaacaaca gccaaacggt gttgatctgg
     5581 aagggctggc gcgtcatcgc ggttcggcgt ttggtcgcac gttacaacca caacttagcc
     5641 aggcgagctt tgaaaacctg ctggctgccg aaatgctaaa aaccgacgcc cgtcaggatt
     5701 tgcgcctgtg ggtgctggaa gatgaaagcc ggatgatcgg ttcgaatcac ctgccggaat
     5761 gtctgcgcga gcggatgact caggcggcga ttgcggtggt agaagatccg tttgagatcc
     5821 gtcttgaacg cctgaacgaa gagtatttct tgcgtatgca tcatgatttt acccacgctt
     5881 atggcgacga acagggctgg caggaatatt gcgaatacct gcatcacgga ctttcggcga
     5941 ttaagcgtcg gctggggtta cagcgctata acgaactcgc tgcccagctg gatacggcgc
     6001 tgacaacgca actcaccacc ggcagcaccg acggtcatct ggcctggctg gtgccgttac
     6061 ttaaagaata ttacgaccca atgtatcgct atcagcttga gaaaaaagcg gaaaaagttg
     6121 tctttcgcgg tgagtgggcg gaagtggcgg aatgggttaa ggcgcggtaa tgagggacgt
     6181 atttgtatta gtcgcgactt gatctgacac atggccttga aaggttgaga gttaccggtt
     6241 ttgatatgag tgtcgaatcc ttatccaaaa caagaggtaa ctctcatgct tcatactgcc
     6301 aatcccgtca tcaaacacaa agccggtttg ctcaatctgg ctgaagaact cagcaacgta
     6361 tcaaaagcct gtaaaatcat gggcgtttcg cgtgatacgt tttatcgtta ccgcgaactg
     6421 gtcgctgaag gcggtgtgga tgcgcagatt aatcgtagtc gccgcgctcc taaccttaag
     6481 aaccgtaccg atgaggcaac tgaacaggct gttgttgatt acgccgtcgc tttcccgaca
     6541 catggtcagc accgggccag caacgagctg cgtaaacagg ggggttttat ctccgactgt
     6601 ggtgtccgtt ctgtctggtt actccataac cttgagaatc tcaaaagacg atactgaaca
     6661 aattttatca ggtgacgttc cgtaaaaagt tgtatggagt tctcgatgca gtcggcgcga
     6721 ctatcagtac ccccgctgaa ccctggacta tcgggccgcc gtactggggc atgtcaccct
     6781 ggcgtgccgc tggttttccg ctaatggttt atttcctctt tctttcccgg caagaattta
     6841 ttttaatccg gaaggattat ttattttcag atagggactc acaataaaca cgtaacattt
     6901 taattaatgc atggctgaat atgtattaca caatgtcaga tatcgtaaaa gaccagtgca
     6961 attctatgta aactctctga ctgttcattt tatttgttgt ttcagggtcg gaactcaccc
     7021 tcaataaaca actgaatacg agtagatatt catcgtctga gctatatggc tttacacaat
     7081 agccgacatg tcttttctca gtcaggaatt ttacttttcc tcactacgaa gttgacgact
     7141 ttgatatggg atagactctt aattcaagca attactctgc acaagcatca ggcaatccag
     7201 ctccaatatt tgttataaaa taatgcgtaa cattaatttc accaacaaaa aaaaggttta
     7261 caccatcccc ttgccctggt atcttaccat atttacacca cacttttttt gtatctggat
     7321 cattctttga tacgattttc gccacttgtt ccctggccct cactgtcaat gttttcttaa
     7381 acctac
//
LOCUS       DAZGVQ010000218         7310 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000218 DAZGVQ010000000
VERSION     DAZGVQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.220"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            120..350
                     /gene="chaB"
                     /locus_tag="ACHLAG_004760"
     CDS             120..350
                     /gene="chaB"
                     /locus_tag="ACHLAG_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFP0657855.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            508..1203
                     /gene="chaC"
                     /locus_tag="ACHLAG_004761"
     CDS             508..1203
                     /gene="chaC"
                     /locus_tag="ACHLAG_004761"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFP0657856.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(1247..1600)
                     /gene="ychN"
                     /locus_tag="ACHLAG_004762"
     CDS             complement(1247..1600)
                     /gene="ychN"
                     /locus_tag="ACHLAG_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFP0657857.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            1785..3179
                     /gene="ychO"
                     /locus_tag="ACHLAG_004763"
                     /gene_synonym="iatA"
     CDS             1785..3179
                     /gene="ychO"
                     /locus_tag="ACHLAG_004763"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFP0657858.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(3180..3830)
                     /gene="narL"
                     /locus_tag="ACHLAG_004764"
     CDS             complement(3180..3830)
                     /gene="narL"
                     /locus_tag="ACHLAG_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFP0657859.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(3823..5619)
                     /gene="narX"
                     /locus_tag="ACHLAG_004765"
     CDS             complement(3823..5619)
                     /gene="narX"
                     /locus_tag="ACHLAG_004765"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFP0657860.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSQAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            5958..>7310
                     /gene="narK"
                     /locus_tag="ACHLAG_004766"
     CDS             5958..>7310
                     /gene="narK"
                     /locus_tag="ACHLAG_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFP0657861.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     V"
BASE COUNT         1698 a         1792 c         1938 g         1882 t
ORIGIN      
        1 ttagacaatg tttgttttag cgtttcttaa gaacagtctg acatgaaaat tcttatgttt
       61 tggcaagagt agatattgtt gaccacactt aatgttcaac tttgtaaaag gagtcaacga
      121 tgccgtataa aacgaaaagc gatctgccgg aaagcgtaaa gcacgttcta ccgtctcatg
      181 cccaggatat ctataaagaa gcgttcaaca gcgcatggga tcaatataaa gataaagacg
      241 atcggcgtga tgacgccagt cgcgaagaaa ccgcgcataa agtggcctgg gctgctgtga
      301 agcatgaata tgccaaaggg gatgatgata aatggcataa aaaatcgtaa aaccggtagc
      361 ttagttaaag ctattcgtgc ggtgttgcct tgcaagtggt ccgtgaattg catattgtcc
      421 cgttagtggt ttcaaaatga gcagtaaaaa tgtccggaag acaccaaaaa gttgtcgcag
      481 ggaagtatgc agtggcggag gtgtaaggtg ataacgcgtg atttcttgat gaatgccgat
      541 tgtaaaacgg catttggtgc cattgaagaa tcactcttat ggtcagcaga gcaacgggcg
      601 gcttcgctgg cggcgacgct ggcttgtcga cctgatgagg gaccggtgtg gatcttcggt
      661 tatggatcgt tgatgtggaa tccggcactg gagtttaccg agtcgtgcac cggtacactg
      721 gttggatggc atcgcgcgtt ttgcctgcgc ctgaccgccg ggcgcgggac tgcgcaccag
      781 ccgggacgga tgcttgcact gaaagagggc ggacgcacta caggcgtcgc ctatcgactg
      841 ccagaagaga cgctggagca ggaactaacc ctgttgtgga agcgcgagat gattaccggc
      901 tgttatctgc caacctggtg tcagcttgat cttgatgatg gatgcacagt aaacgccatt
      961 gtgtttatta tggacccgcg acatccagaa tatgaatctg acactcgcgc tcaggtcatc
     1021 gcgccgttga ttgcggcggc gagcggtccg ctgggaacca acgcacaata cctgttttca
     1081 ctggaacagg agcttatcaa actgggaatg caggacgatg ggctgaatga tttgctggta
     1141 tcggtaaaaa aactgctggc ggagaattat ccggatggtg tgttacggcc gggattcgcc
     1201 tgagtaaact tccctcatag tggggcgtca gacgccccct caaacattaa aatgtgagca
     1261 ctttatcggc tgacagcgtc cattgcgcca gttccacaag agtaccgatt tccaccccat
     1321 caataagagg aagtgtgcta atcccgcgcc cgtcggtaca ggttttgcac aatttcaccg
     1381 gtacattctg agcggtaagg atctccagca tttgctgaat gttgtagcct tcccctggtt
     1441 tttgcccgcg caacccggct gtgaccgcat cagacatcag gaacagacgc agatccagat
     1501 tgctctcctg ctctcgtaac gcaatggcca gccgcaagct gttaaacaag gattcgctcc
     1561 cgtaaggtgc gccattggca acgatcacga ttttttgcat tatttactcc tgtattcagg
     1621 gaattagaca ttcatcttct atcttactgc ttctgcagcg tctgaccaat cggtcacatt
     1681 tttaaggatt ttcctgaaag cgcgagaaaa tacgacaaaa gttgccagta atcgttattc
     1741 tttaaggcta tggtttttca ttattaccgg aagttaccga cgttttgagc cgtttcgttc
     1801 ctcgcattat cccgttttat ttactcttgc ttgtagcagg cggtacagct aacgcacaat
     1861 ctaccttcga gcaaaaagcg gcaaatccct ttgataataa caatgatggt ctgccggatt
     1921 taggcatggc tcccgaaaat catgatgggg aaaaacactt tgctgaaatt gtgaaagatt
     1981 tcggcgaaac cagtatgaat gataacgggc tggatactgg cgagcaggca aaagctttcg
     2041 cattgggaaa agtccgcgac gcgcttagtc aacaggttaa tcagcacgta gagtcctggc
     2101 tatcaccgtg gggaaatgcc agtgttgacg tcaaagtgga taacgaagga catttcaccg
     2161 gcagtcgtgg aagctggttt gtgccgttac aagataatga tcgttatctc acctggagcc
     2221 agcttggtct tactcagcag gatgatgggt tggtgagcaa tgtgggcgtt gggcaacgct
     2281 gggcgcgcgg caactggctg gtgggttata acacttttta tgacaacttg ctggacgaaa
     2341 atcttcagcg agcgggcttt ggtgccgaag cgtggggcga atatttgcga ctatcggcaa
     2401 acttttatca gccgtttgct gcatggcatg aacagacagc cacgcaggaa caacggatgg
     2461 cgcgcgggta tgacctgaca gcccggatgc gcatgccgtt ctatcaacac ctcaatacca
     2521 gtgtcagcgt agaacagtat tttggtgatc gtgttgattt gtttaactct ggtacgggtt
     2581 atcacaatcc cgtcgcgttg agtctgggat taaattacac ccctgtgcca ttagtcactg
     2641 tgacggccca gcataaacag ggtgaaagtg gcgagaatca aaataacctc gggctgaatc
     2701 ttaattaccg ctttggtgta ccgctcaaaa aacaactttc tgcgggtgag gttgccgaaa
     2761 gtcagtcgtt acgtggtagt cgctatgaca atccgcagcg aaataatcta ccgactcttg
     2821 agtaccgaca gcgaaaaacg ttaacggtgt ttctggcgac accgccgtgg gatctaaaac
     2881 ctggcgaaac agtgccgctg aaattacaaa tccgcagtcg ttacggtatt cggcaactga
     2941 tttggcaggg cgatacgcag atattaagtt tgacgccggg cgcacaagcc aacagcgcgg
     3001 agggctggac gctgatcatg cctgactggc agaacgggga aggggcaagc aatcactggc
     3061 gattgtcggt ggtggtggaa gataaccagg ggcagcgtgt ctcctccaat gagatcacgc
     3121 taacgcttgt cgaaccgttc gatgcattgt caaacgacga actgcgctgg gaaccgtaat
     3181 cagaaaatgc gctcctgatg cacccatacc gctgcttcca cgcgagactt gagcttcatt
     3241 ttcttcagca tgtgcttgac gtgcactttt actgtgcttt cggtgatatc caggcggcgg
     3301 gcaatcatct tgttcggcaa accctgggca atcagcttga gaatatcgcg ctcgcgtggg
     3361 gttaactggt taacatcgcg ctcagtagtg gcacggttag cgcgcaagct ggcggccaga
     3421 acaggcgtta atgcttcgct taataccatt tcgccagcag ctgcctgatg caatgctttc
     3481 agcagatctt ccggttccat atcttttaac agatagccat ccgcgccgcg tttcagtgcg
     3541 gtgaccacat cttcttcatg gttagagacg ctgaatacca caatgcgccc tgagagggac
     3601 ttttcgcgca gtttatccag cgtttccaga ccgttcatgc cgggcatatt gagatctaac
     3661 aggatcagat cgggatcaag agattccgcc agttcaatac cctgttcgcc attactcgct
     3721 tcgccaacca cggtgatatc tggtgccata ctgataagct gttttacgcc agttcgcagc
     3781 atcgggtgat cgtcaatcag caggatagta gccggttcct gattactcat gggtatctcc
     3841 ttggacgtct gtgaaagttt tttcgggaat aaaggtgacc accacttcgg tgccacctga
     3901 ttcacgacgg cggacgcggc aatcgcctcg taaactttgc gcacggtcgc gcattattat
     3961 catgccgtag tgattgctgc ggatggcatt ttcaggcacg ccgcagccgt tatcctggac
     4021 ggtcagtttg acctgattat cgttttgcgc caccgtcacc acgacttcac tcgcttgcga
     4081 atgtttgagg gcgttactta atgcctcacg ggcaatttgc aacaagtgga ttgcctgatg
     4141 cgaaggcacc aggcgaggcg gcaattgata atccagcttc accgggaagc caaatttggc
     4201 gctgtactct tcgcaactcg cctccagcgc cggacgtaat ccaggctcgg tgagctgcaa
     4261 gcggaatgtg gtgagcaatt cacgcaactg cgcccaggat gcattcagtt cgttacggat
     4321 ctgacttaac agttcgcggc tgctttctgg cagcgcatcg ccctgcatct gtaaacaact
     4381 cacctgcatc ttcatgcaag agagagattg ggcaatagaa tcatgcagtt cgcgcgcaat
     4441 ggtggcacgc tcttccatca cgatcaactg ttgctgacgt tcctgatggc gatccagcgc
     4501 cagcgtggcg gtgagttgtt caaccagggt atccaccagt tgttgttgat catggctaag
     4561 atgacgcccc tgcggcaggg tcgccagcaa aataccgtac tgcgtatgag agtcagccag
     4621 ccgccacttc agggttgtgc cgcgatcacc aacgggtaat acgccgcgcg ggcagagctg
     4681 gcagccttta tcatcacaag tcatatctgg ttggcaggta aactcctgat gattctcttc
     4741 atcatcagta tcataaaccc gcagttcgat atcacgtagc agggttaaat tctgtaagcc
     4801 gttgagtaca ggtgacaggc gttcacacag cggggcttgg gaatgcaaac ggcggttagc
     4861 ctgccataaa aaagagagga tctgattttt atgctccagc ccggcggttt tctcctgaac
     4921 ccgctgctca agtacggcat aactttcggc cagttctgca gacatattgt tcaacgcagt
     4981 tccaagcatc gccatttcgt tgcgcccgct gatgtttgcg cgttgggtaa aatcgcgatg
     5041 actgacggca ctcgccattg ccagcagttg ccgccacggt tgtagcagtc gcgcccgcaa
     5101 ccagataata gtgaacacca gtaaaagtgc cataaatacc gccattaccc gatggaccag
     5161 taccactgtc tcgatgcgca tttccgtggt gcggtcaaaa ccagatacca gttgatcaag
     5221 cccggcaaca aactggctga catccgctga caccgtttct cggttttgtg cacgcatcaa
     5281 cgcagggatc agttcattac gccagtaatc ttgtaaaccc tgtaattgcg ccagttgtcc
     5341 gtctcgttct gctgctcgag tcaactcggc gctaaatgcc gtttgttcca tctctttaat
     5401 taagggcttg tctttctcgc ttaatggcac tgctgccaac agacggtaac tttgcatgcg
     5461 cagcgatccc gctttgttga tcgcatgggc gctgccctga acgccttgca ccagccagcc
     5521 agaaaccgcc atccctgcca gtccaatagc agtagaaagc aacacaataa gcgcaacctg
     5581 attaaccagg gtgagcggag agagacaacg tttaagcatg taaacctctt ccttcaggct
     5641 ttaaatgagc aataacctta atgaatgtga cgatacattc tggaatggca gtattctcgg
     5701 ctattggctg aagtataccc atacccggaa agagttactc cttatttgcc gtgtggttag
     5761 tcgctttaca tcggtaaggg tagggatttt acagcactgt gaaaaatctc ataattttta
     5821 tgaaatcact gtactcacta tgggtaatga taaatatcaa tgatagataa agttatctta
     5881 tcgtttgatt tacatcaaat tgcctttagc tacagacact aaggtggcag acatcgaaac
     5941 gagtatcaga ggtgtctatg agtcactcat ccgcccccga aagggctact ggagctgtca
     6001 ttacagattg gcgaccggaa gatcctgcgt tctggcaaca acgcggtcaa cgtattgcca
     6061 gccgcaacct gtggatttcc gttccctgtc tgctgctggc gttttgcgta tggatgttgt
     6121 tcagcgctgt tgcggtgaac ttaccgaaag tcggctttaa ttttacgaca gatcagctat
     6181 ttatgctgac tgcgctacct tcagtttctg gcgcgttatt acgtgttcca tactccttta
     6241 tggttccaat cttcggtggt cgtcgctgga cggcgttcag caccggtatt ctgattattc
     6301 cttgtgtctg gctgggtttt gccgtgcagg atacctccac gccttatagc gtcttcatca
     6361 tcatctctct gctgtgcggc tttgctggcg cgaacttcgc ttccagtatg gcaaacatca
     6421 gcttcttctt tccgaaacag aagcagggtg gcgcgctggg cctgaatggt ggtctgggca
     6481 acatgggcgt tagcgttatg cagttggttg ctccgctggt ggtatcactg tcgattttcg
     6541 cagtatttgg tagccagggc gtcaaacagc cggatgggac tgagctgtat ctggcgaatg
     6601 cgtcctggat atgggtgccg ttccttgcca tcttcaccat tgcggcgtgg tttggcatga
     6661 acgatcttgc tacctcgaaa gcctccatca aggagcagtt gccggtactc aaacggggtc
     6721 atctgtggat tatgagcctg ctgtatctgg caaccttcgg ctcctttatc ggcttctccg
     6781 cgggctttgc gatgctgtca aaaacgcagt tcccggatgt tcagattctg caatacgctt
     6841 tcttcgggcc gtttattggt gcgctggcgc gttctgcagg tggtgcatta tctgaccgtc
     6901 tgggcggaac tcgtgtcacg ctggtgaact ttattctgat ggcgattttc agcggcctgc
     6961 tgttcctgac cttaccgact gacgggcagg gcggaagctt catggcgttc ttcgcggtct
     7021 tcctggcgct gttcctgaca gctgggctgg gtagtggttc cactttccag atgatttcag
     7081 tgatcttccg taaactgaca atggatcgcg tgaaagcaga agggggttct gacgaacgtg
     7141 cgatgcgtga agcggcaacc gacacggcgg cggcgttggg tttcatctct gcgattggcg
     7201 cgattggtgg cttctttatc ccgaaagcgt ttggtagctc gctggcatta acgggttcgc
     7261 cagtcggcgc aatgaaggta tttttgattt tctatatcgc ctgcgtagtg
//
LOCUS       DAZGVQ010000219         7219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000219 DAZGVQ010000000
VERSION     DAZGVQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.221"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1153)
                     /gene="ycjT"
                     /locus_tag="ACHLAG_004767"
     CDS             complement(<1..1153)
                     /gene="ycjT"
                     /locus_tag="ACHLAG_004767"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFP0657862.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTIARSLLRYRWHNLPGAQEKARRNGWQGALFPWESA"
     gene            complement(1150..2205)
                     /locus_tag="ACHLAG_004768"
     CDS             complement(1150..2205)
                     /locus_tag="ACHLAG_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP0657863.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(2215..3003)
                     /locus_tag="ACHLAG_004769"
     CDS             complement(2215..3003)
                     /locus_tag="ACHLAG_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP0657864.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRTLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLTWLRTC"
     gene            complement(3021..4073)
                     /locus_tag="ACHLAG_004770"
     CDS             complement(3021..4073)
                     /locus_tag="ACHLAG_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFP0657865.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FCAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(4104..4946)
                     /locus_tag="ACHLAG_004771"
     CDS             complement(4104..4946)
                     /locus_tag="ACHLAG_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP0657866.1"
                     /translation="MATNKRTLSRIGFCCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(4933..5814)
                     /locus_tag="ACHLAG_004772"
     CDS             complement(4933..5814)
                     /locus_tag="ACHLAG_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP0657867.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(5835..7127)
                     /locus_tag="ACHLAG_004773"
     CDS             complement(5835..7127)
                     /locus_tag="ACHLAG_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP0657868.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
BASE COUNT         1685 a         2021 c         1833 g         1680 t
ORIGIN      
        1 gcgcgctttc ccacgggaat agcgcgccct gccagccatt gcgtcgcgct ttctcctgcg
       61 cgccaggcaa gttgtgccag cgataacgca gtaaacttct ggcaatcgtc ggatcgctaa
      121 acagatggaa cggcagcaaa aatacttccg tatcccagaa aacgtggcct ttgtagcctt
      181 cgccggttaa gccttttgcc gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc
      241 gcagatgata aagcgcataa tctaacgctt gctgatcgtg cgcttcgccg ccatttaccg
      301 tgatacgacg tttctgccac cattgacgcc agttttctgt tgatactgca agaagttggt
      361 cgtaactctg ctgcgcgcac atttcaagct ggcgaagcga cgcgctgccc cactcgtcta
      421 aaacggcttg cctgtcatcc cgccagtcga tccacaccag tttttgcaac gtcactgtct
      481 cgcctgcatg aagctgcgca ctggtatgtt gctgcaaacg gcgctctttg gcggtatagc
      541 attgctgcac atcaccgctc accatacagc aacagctgat ggccacatca ctgcgtccat
      601 cctgggtggt gtagatcccc tgcatcagat gctgaccaaa cacccgcacc tgggtttcgt
      661 cgagatgttg gcgaccgtgg ttggtttgcg tggcgtcgat acctgttgaa atcagcactg
      721 acgcgtcggc gtccagtggc gtaatagtga tttccagcac aatgagcggc agttggtctg
      781 ccgaaacaaa gcgacggctg gcgatggtgt aacctgtgcc gttgctggta cgccagacaa
      841 cgctacggcg taattctcca ctggcaaagt caagttcacg ctgccaggct tcgcgggata
      901 acgagaaaac ctcaccattt atggcaatct ccatccccaa gatatcaggc aggttcacca
      961 gttcgttgat ttcacctttt cccgcccgat gatacagccc cgccagatac atccctcgcg
     1021 tctggcgcgt gtaatcttct tcatggctgg cgcgaagccc aagatagccg ttcccctgcg
     1081 ccatcagcga tgcatacttg ttcagggtat gctggctgaa atggggttct gataacgtta
     1141 ctggcctggt cataattcca cacgcgtccc tgtttctgcg gattgataca acgccgccac
     1201 cagttgctgg atgatgtacc cctgctcggc gtcggctatc atcacgggct tgccctgtac
     1261 gtgattgata aaggcttcca tgctgcgcaa atggcggttg tcgtctgcca gttcccgttg
     1321 catcagcgtc attaattcac cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc
     1381 accggcttta tcaccacaaa agctgacgtt cataatcgac tgttcgcgga tgttgagtgc
     1441 aaatgacgtt tccagccaca gaatgccgcc gttatgaaat tcaatggtgc caaacagcga
     1501 atcttcgacg ctataagttg ccggatccca ctcgccaaat tgaccacagc tcttttgcgt
     1561 gccgatcttt tgaaagctat gcgcattcac gcttttcacc gccggaaagc ccagcacata
     1621 catcgcagca tccagcatat gaatgccgat gtcgatcagc gggccaccac cctgcagttc
     1681 tttattggta aagacgcccc agccgggaac gccgcagcga cgcagggcgc gggcggtggt
     1741 aacgtaaatc tctcccaaaa cgccgttggt cacctgttca cgcagctgtt gcgtatcgag
     1801 cgcaaaacga tggtgaaagt cgtaggccag caccttgccc agtttgcgcg cggtatcgca
     1861 catttcccgc gcctgttctg gcgtcatggc gggcggtttt tcgcacatca catggcagcc
     1921 cgcttccagt gccatcaggg tatgttcgta atgaaaacgg ttaggtgagc agacgctaac
     1981 cacatcaggt ttcaccgcca gcagcatggc ctgcggatcg tcccacacgg atgcattccc
     2041 gtatttttct gccagcgcct gcgcctggga aaggcggctg tcacagacag ccaccagttc
     2101 cagatcgttg cgggtgcagt agtacgaagc atgaacctta tccgccacct ggcctgcgcc
     2161 tattatcgcg acccgcagtg gagagcttgt cattgcactt ttcacaaaga cctcttagca
     2221 ggtacgcaac caggtcaacg aatcacggta cgcctgggca ggatcttctg cccggatacg
     2281 cccttcatac accacataac cctgatagtt atcggcgcgc agctgttcaa acagcgcgtg
     2341 gaaatcgagg gtgccgctgc ccggctggta acgatggtta tcggcaatat gcacatgacc
     2401 gagcaggtcg cggttgtcgt gcagcgcctg cgccaggtta tcttcttcga tattcatgtg
     2461 atagaaatcg ccgataatct gcacatgttt aagatcgttt tcgacgatgt aacggcgggc
     2521 atcggcgaga gtgttgatca tatgatcctg atagcggttt aacggttcga gatacaccac
     2581 ggttccggta cgcgctgcca cttgttccag cgtgcgcagg gaatcgctca ccattttacg
     2641 atcgccgtcc aggctgcgcg gcgaggtcat cggcggtaag cggaaggtaa acatgcccca
     2701 cgctgccgga acgacgatac ctttaccccc cacttctgcc agcgcttcaa gaatgcgttc
     2761 gatctgcttt aagccattaa gacgacgctc ttcgataaag tcaccaatcc agccgtcata
     2821 gccaccgcag gcggtggtca ccggtaaacc ggtttctttg attgctgctt tgacttcttc
     2881 gagattgttg accagcagtt tgccgtcaat ctcaaaacca tcgaagccca tctctttgat
     2941 ataacgaaat ttctccagaa tgttttccgg aaagaatgcc tgattctgtg tgcctatttt
     3001 cattttttca ttcctttgaa ttaaaacgta acgcccattt tgatgctctg ttccggatgc
     3061 tggtcgacat actgcatgta gctttccggg ctattggcaa aggtcaccac cgggtcgatt
     3121 agatcttcac aattgagata accgttcatc agcagttccc aacaggtttc ttcgatacgc
     3181 ttgcggctcc agcgcggata atccgggttc ggttcgctgc acgcgcggga gaagacaatt
     3241 ttggcgttat tgaaatgggc ttcgcgtccg aggttaaaac cttcggcaaa cggtttggca
     3301 aacgcaacat acgagatggt gccgccataa gccaggccgc ggagcgccga ttgcagcgcg
     3361 tcggcgtaac cgctggtttc gataatcaca tcggcaccct gcttgccggt cagcgttttg
     3421 atctctttac ctacgtcagt gccaatgggg ttaaggcaga aatcagcacc gtggcgacgg
     3481 gcaatatcac agcgataggc gataggatcg acgccaatca ccaccgacgc gccagcgcgt
     3541 ttcgccagtt ggatggctat ttgaccaatc gcgccaagcc ctaccaccac cacaaaatca
     3601 cccacgcgca cgttggcatc gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacag
     3661 acggcgtttt tccaggagct gccttgcggc attttgcgca gcttgtagtt attcaccgcg
     3721 ttaatgatga ccgtctcgga gagtgggccg tagccgcata cgctgtcgcc caccgcgtag
     3781 tcggttacgt cgctgccgca ctcgataatg tcgccaacca ccatgttgcc aagctggaat
     3841 ttgccaaact caatgccgcg cggcgcatct gccggacgcg gagtgaacat ctgccattca
     3901 ccgttaaagt cttcatcgat aaacgggctg gcggcgcaga agtcgaccac ttccgttccg
     3961 tgtttcggtg cgccgaaacg cacgcggatc ttaacttcat tggctaaaat cgcccgatct
     4021 tcatactcaa ccagcgcagc aacgcgcggt gctgtggcta ctaacttttt catttaaaac
     4081 tccttgtaaa gaaactggcc gctttagccc ttcacgccac cggcggtcaa accactctta
     4141 ataaaacgtt ccgaaagcgc atacataatc accaccggca atgcggtcac cagtgaggcc
     4201 gccatcatcc gtccccagat gtaatctggc gtactgaaca gtgcgttcag gcccaccggt
     4261 aaggtgaaat tgctggcgct ggaaaggaaa atcgacgcaa acagatagtc gttccacgcc
     4321 accataaagc agtagacgaa cacggatatc agcccggaca tcgccagggg cacggtgata
     4381 cggaagatga tttgcagacg gttgaggcca tccatcatcg ccgcttcttc gatctcatca
     4441 gggatggtgt cgaagtagct tttcagcata aacacggcgg tgggcagggt ttgcgtcacc
     4501 atggtaatga tcagcgccat ctcggtgtcg taaatgccca gcgcggtgat gattttgaac
     4561 agcggcacca ccagcaaaat gccagagaac atatacaccg tgtaaaagct ggcattgatg
     4621 gtcatccgac ctttaaagcg caggcgggaa agcgcgtaag ccccaagaat gccgaggaat
     4681 accgccacca cggatgaaac caccgacacc accagactgt tacggaagta gtcgacaaac
     4741 gggaaaatca tcgggttaaa aatgtcgacg taatgctcca gcgtccactg ctgcggcagc
     4801 agcgtaggat gcagtgagat cgcctctttc gcgcccttga acgaggtcat cagcatcaca
     4861 aaaaatggaa acagcgtgat gatgagaaac agcgccagcc cgcagcaaaa accgatgcga
     4921 ctgagtgtgc gtttatttgt tgccattgag gttcacccgt ttcctggtca gcagaatgac
     4981 agcgaaaata atgatgaaga gcaccaccga gatcgccgcc gcttttccta aatcattaaa
     5041 ggcaaaggcg gttttgtaga gatatacacc gagaatatcg accttggtcg tcagcaaata
     5101 aacatccgcg aacatgtaga acatccagat ggtgcgcagg gtcaccaccg tcgccaggac
     5161 cggcataatc gcgggcagcg tgacgatacg aaaccgttgc caggcattag cgccatccat
     5221 ctctgccgct tcgtacagcg atttgtcgat ggtctgcaaa atcgccagaa acgagataaa
     5281 ggcatacggg aagtagcgcc agatggcgaa cagcaccacc agcacaaagc tactgcccgg
     5341 attatcgaac cacagcggtg cctgctcata gagatgcaga agatcgacgc cgaggtagtt
     5401 cacaatgccg tagccgttgt tgaacatgta tttccaggca aacaccagcg aaatggacgg
     5461 cgttacgtag gagaggatca ccagcgaacg cgcggttttg cgcagacgga actcgcggtt
     5521 gaaaaacatc gccactgcca gcccgagcac ggtgctgccc gccaccacca gcgcggtata
     5581 ccagaccgtc atccacagcg agtgccagaa gccgggatcg gagaggatac gcacatagtt
     5641 actcacccca acaaaggttg actcgatgtt gggattgagc ggcagacgta aaaaactgat
     5701 ttcgatattc gacaccattg gccaggccac cagaccaccc agcagtaata agctgggcgc
     5761 gagaagcagc agcgcaaagg gcatatcgga acgacctgaa aaaagcctgt tcattgcgga
     5821 ttcctgtcct gggtttagtg ctgttcgatc agttcatcca gctttttctg gctcgcttgc
     5881 agcgtgccag ggagatcggc tttacccacg gtgacgttat gcaccatgga gctcaccacg
     5941 ccagaccccg tcacatcacc catgcgggta aagtttttat cccctactgc accaaagacc
     6001 tgaatatttg gcagttcacc gattagctga ttcggtagtt cacccagcgc cttaataacg
     6061 tcgttgtctt tccaggtggc ggtagtgaca accgctttat tcaccggcag cgcagcacct
     6121 ggcgacatca tcacccagtc ggcaatgttg tctgcctgct ccataaaggt gacaaatttt
     6181 tctgctgctt ccgtctcttc ggtcttttgc ccggcggtaa tggtcagcga ggtcaacatg
     6241 ccgtagaccg cagagttttt ctcggttggc accacgaaac cgacgttttt cgggtcgcct
     6301 tctttaatca cagccggaag gatataggtg gagtaaatcg ccatcggcgc ggtgccgttc
     6361 ataaaggcgt ctttcacttc catgatgtcg ttagaacccg gcatagtgtt ggcagcaagg
     6421 tcgcggtaat aggtcagtgc ctgcatcatc tctggtgtat caagggtgat tttgccttcg
     6481 gcgttaaaga cgttagcctg gttggataac gcaaactggg agaaggattg ttccgtcaac
     6541 acgctttctg ctgtaggcag cgcaatgccg tattttttat tcgccgggtc attcagtttc
     6601 tgtgcaacgt ccagcagctg ttgccagttt ttcggctcct caagccccgc ttttgccagc
     6661 acatctttgc gataccagat accgccaatc caggcgctga caggaacacc ggtccatgcg
     6721 ctaccatctt cggtacgcac aatacgcagt acgccatcgt aaaacgcgcc ttcaccaacg
     6781 ttgctgatga ctgtggcaac cgctttgcga tcgataagct gctctttgtc catcactttg
     6841 gcgtagtcat ggctggtttc tatcacttcc ggcagcgaac cgctacgtga aagagtaatg
     6901 actttagtgt tataggcatc ttcttccacg ggcacttgct taacggtgat gccagggttt
     6961 tctttttcaa aacgggcaat caatttactg ataacggcct ggcgctcctg ctccaccgaa
     7021 gaatgcataa attcgatgga tacattcgtt ttattttctt ctttacagcc tgaaatcagg
     7081 gcgcaggaaa ccagtgctga taacagcacg atttttgatt taatcatgag atgttccttt
     7141 ttatttaatc cacataacct gccagaggcg cagcgttatt tcattaccca atatatcaac
     7201 ttcactaatc agatcccga
//
LOCUS       DAZGVQ010000220         7041 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000220 DAZGVQ010000000
VERSION     DAZGVQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.222"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            342..1379
                     /gene="dusA"
                     /locus_tag="ACHLAG_004774"
     CDS             342..1379
                     /gene="dusA"
                     /locus_tag="ACHLAG_004774"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFP0657869.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            1513..1755
                     /gene="pspG"
                     /locus_tag="ACHLAG_004775"
     CDS             1513..1755
                     /gene="pspG"
                     /locus_tag="ACHLAG_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFP0657870.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRDDRWRY"
     gene            complement(1921..2904)
                     /locus_tag="ACHLAG_004776"
     CDS             complement(1921..2904)
                     /locus_tag="ACHLAG_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFP0657871.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNII
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            2987..4402
                     /gene="dnaB"
                     /locus_tag="ACHLAG_004777"
     CDS             2987..4402
                     /gene="dnaB"
                     /locus_tag="ACHLAG_004777"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP0657872.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4455..5534
                     /gene="alr"
                     /locus_tag="ACHLAG_004778"
     CDS             4455..5534
                     /gene="alr"
                     /locus_tag="ACHLAG_004778"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFP0657873.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            5787..6980
                     /gene="tyrB"
                     /locus_tag="ACHLAG_004779"
     CDS             5787..6980
                     /gene="tyrB"
                     /locus_tag="ACHLAG_004779"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFP0657874.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLKQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHIAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVMCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1659 a         1746 c         1879 g         1757 t
ORIGIN      
        1 tggaaggcat tacataaaag aacctaatac ttattgggtt cttttttctt ctatcagtgc
       61 cattagcagg aagagatatc accggagttt aatgtgtgat tttttattta tcgtcgaacc
      121 tggattgttt atcattgtcc ttaacaaggc taacggctaa taagattatt tccatcactt
      181 cgtgagagct tcatgccttg atttgatctc aattttcttt tgcaatgaga caggcgcttc
      241 ctgttgttat ggtatagtac cccgctattg agcctcctga acagtgatgc tgaataacat
      301 aacccaatga tatatcgata aaataatctc tacatttgaa aatgcacggt aattctgaaa
      361 tgcaaaaaat caaccaaacc agcgcaatgc ctgaaaaaac tgacgttcac tggagtggtc
      421 ggtttagcgt tgcaccaatg ctcgactgga cggacagaca ttgccgctat ttcttgcgtc
      481 tgctttcccg caatacgttg ctgtataccg aaatggtgac cacaggggcg attattcacg
      541 gtaaaggtga ttacctggcg tacagtgaag aagaacatcc ggtagcgttg caactcggcg
      601 gtagcgatcc ggcggcgctg gcacagtgtg cgaagctggc agaagcgcgt ggatatgatg
      661 agatcaacct gaatgtcggc tgcccgtctg accgggtgca gaacggcatg tttggtgcgt
      721 gtctgatggg taatgcgcag ctggttgccg actgtgtgaa agcgatgcgc gatgtggtgt
      781 cgattccagt gacggtgaaa acgcgtattg gcatcgatga ccaggacagc tatgaatttc
      841 tctgcgattt catcaacact gtttccggca aaggcgagtg tgagatgttt atcatccacg
      901 cacgtaaagc ctggctttcg gggttaagcc cgaaagaaaa ccgtgaaatc ccgccgctcg
      961 attatccgcg tgtgtatcaa ctgaagcgtg actttccgca tctgacaatg tcgattaacg
     1021 gtggtatcaa gtcgctggaa gaggccaaag cacacctgca acatatggat ggcgtgatgg
     1081 tcgggcgcga ggcgtatcag aatccgggta ttctggcggc ggtagaccgg gagatctttg
     1141 gttcctcgga taccgatgcc gatccggtgg cggtagtgcg cgccatgtat ccgtacattg
     1201 agcgtgaact cagccagggg acgtatctcg gccatattac ccggcatatg ctgggtttgt
     1261 tccagggtat tcctggcgcg cggcagtggc ggcgttattt aagtgaaaat gcccataaag
     1321 cgggtgctga cattaacgtg ctggaacacg cgctcaaact ggtggcggat aagcgttaac
     1381 ttttcaccaa aaagtagtca aattcaccac gccctgcgca ccgtcgcggg gcgttttgct
     1441 gttaaatcaa tagattattt ttggcatgat tcttgtaatg ccagcaagag atttcatatt
     1501 tgggagagca tcatgctgga actacttttt gtgattggct tttttgtcat gctgatggtc
     1561 accggcgttt cgttgctggg cattatcgcc gcgctggttg tggcgacggc cattatgttc
     1621 ctcggcggta tgctggcatt gatgattaag ttgctgccgt ggttactact ggcgattgcg
     1681 gtggtgtggg ttattaaggc gattaaagca ccaaaaatgc cgaaatatca gcgtgatgac
     1741 cgctggcgtt actaagggat tgtgcggatg atcacaacct aaggttttat ccttagaaca
     1801 aaataggaat tgataatcaa atctgtcact attgcgcctc taacagattc atcgtgctgt
     1861 accctacata cagccgaact ataaaaagaa agggcttccc aggtggaagc cctatttctt
     1921 ttatggaatc agcaggctgg aaccttgcgt tgcccggctt tccagaattt catgcgcacg
     1981 ctgcgcatcc ttcagcggat atttctgctg ctcggcgaca tcgaccttaa tcacaccgct
     2041 ggcaatcaaa gagaacagtt cattactggc ctcggttaat tcctcccgcg tggtgatata
     2101 gccttgcagg gaagggcgtg tcacatacaa cgagcctttc tgattgagaa tgcctaagtt
     2161 cacaccggta accgcacctg atgagttgcc aaaactcacc attaagccgc ggcgttgcag
     2221 gcaatccagc gaccgttccc aggtgtctct gcccacggaa tcgtacacca cgcgcacttt
     2281 cttaccgccg gtgatctctt ttaaccgctc gaccagattc tcttcacgat agttaataac
     2341 ctgccacgcg cccgctttta gcgcgctctg cgctttttgc gcggttccta cggtgccgat
     2401 aagtttcgcg cccagggctt ttgcccactg gcaggcaatc aagccaacgc cgccagctgc
     2461 tgcgtggaac aggaactgct catcgggttt aatttcatag gttttgcgca gcagataata
     2521 aaccgttaag cctttcagga aggatgccgc agcttgctca aaagaaattg ccgcaggcag
     2581 aatcgccgct ttatccgcaa taatgttatg cacagagctg taagcgccta acgccgactg
     2641 cgcatagact acacgatcgc ctgccttaat atgctttaca ccactgccga ctttactcac
     2701 gatgcctgct gcttcggtgc ctaatccgct gggtagcgat ggcggcgggt aaaggccgct
     2761 gcggatatat gtgtcgataa aattgatgcc gatggcttta ttttcgacct ggatttcatt
     2821 ctctgccgga tcggcaggag tgaactctac ggcttgaagt acttccgggc caccgtgctt
     2881 gtgaaattca attcgtgttg ccatgtgtcc tccagaacgt atcgtcaggg tctgcttcat
     2941 atggtaaagt ttcgacccat tctttatctc ggtaactcca ttcactatgg caggaaataa
     3001 acccttcaac aaacagcagg ctgaaccccg cgaacgcgat ccacaagttg ccgggctgaa
     3061 agtgcctccg cactcgatcg aagcggagca gtcggtgttg ggcggtttaa tgctagataa
     3121 cgaacgctgg gatgatgtag ccgagcgtgt ggtagcagac gatttttaca cccgcccaca
     3181 ccgtcatatc tttactgaaa tggcgcgttt gcaggaaagc ggtagcccta tcgatctaat
     3241 tactcttgcg gaatcgctgg aacgccaggg gcaactcgat agcgtcggtg gttttgctta
     3301 tctggcagag ctgtcaaaaa atacgccaag tgcggcgaac atcagtgcct atgcggacat
     3361 cgtgcgtgaa cgtgccgttg tccgtgagat gatttcggtt gcgaatgaga ttgctgaagc
     3421 cggttttgat ccgcaggggc gcaccagcga agatctgctg gaccttgctg aatcccgcgt
     3481 ctttaaaatt gctgaaagtc gtgcaaacaa agacgaaggg ccgaagaaca tcgccgatgt
     3541 gctcgacgca accgtggcgc gtattgagca gttgtttcag cagccacacg atggcgttac
     3601 cggagtaaac accggttatg acgatctcaa caaaaaaacc gctggcttgc agccgtcgga
     3661 tttgatcatc gtcgccgcgc gtccgtcgat gggtaaaaca acatttgcga tgaacctcgt
     3721 cgaaaacgcg gcgatgttgc aggataaacc agtacttatc ttctcgctgg agatgccttc
     3781 agaacagatt atgatgcgtt ctctggcgtc gctgtcgcgc gttgaccaga ctaaaatccg
     3841 taccgggcag ctcgatgatg aagactgggc gcgcatttcc ggcaccatgg gtattttgct
     3901 cgaaaaacgc aatatctata tcgatgactc ctccggcctg acgccaacgg aagtgcgttc
     3961 ccgcgcacgc cgtattgccc gtgaacacgg cggcatcggg cttatcatga tcgactacct
     4021 gcaactgatg cgcgtaccgg cgctttccga taaccgtacg ctggaaattg cagaaatctc
     4081 tcgctcgctg aaagcactgg cgaaagaact gaacgtgccg gtggtggcgc tgtcccagtt
     4141 gaaccgttct ctggaacaac gtgccgacaa acgcccggtc aactccgacc tgcgtgaatc
     4201 tggctctatc gagcaggatg cggacttgat catgtttatc tatcgtgatg aggtgtatca
     4261 cgaaaacagt gatttaaaag gcatcgcgga aattattatc ggtaaacaac gtaacggccc
     4321 aatcgggacg gtacgcctga cctttaacgg tcaatggtcg cgcttcgaca actatgcggg
     4381 gccgcagtac gacgacgaat aataattatt ttatgaatta ggtaattaaa gcaaacactt
     4441 atcaaggaac acaaatgcaa gcggcaactg ttgtgattaa ccgccgcgct ctgcgacaca
     4501 acctgcaacg tcttcgtgaa ctggcacctg ccagtaaaat ggttgcggtg gtgaaagcga
     4561 acgcttatgg tcacggtctt cttgagaccg cgcgaacgct ccccgatgct gacgcctttg
     4621 gcgtagcccg tctcgaagaa gctctgcgac tgcgtgcggg gggaatcacc aaacctgtac
     4681 tgttactcga aggctttttt gatgccagag atctgccgac gatttctgcg caacattttc
     4741 ataccgccgt gcataacgaa gaacagctgg ctgcgctgga agaggctagc ctggacgagc
     4801 cggttaccgt ctggatgaaa ctcgataccg gtatgcaccg tctgggcgta aggccggaac
     4861 aggctgaggc gttttatcat cgcctgaccc agtgcaaaaa cgttcgtcag ccggtgaata
     4921 tcgtcagcca ttttgcgcgc gcggatgaac caaaatgtgg cgcaaccgag aaacaactcg
     4981 ctatctttaa taccttttgc gaaggcaaac ctggtcaacg ttccattgcc gcatcgggtg
     5041 gcattctgct gtggccacag tcgcattttg actgggtgcg cccgggcatc attctttatg
     5101 gcgtctcgcc gctggaagat cgctccaccg gtgccgattt tggctgtcag ccagtgatgt
     5161 cactaacctc cagcctgatt gccgtgcgtg agcataaagc cggagaacct gttggttatg
     5221 gtggaacctg ggtaagcgaa cgtgataccc gtcttggcgt agtcgcgatg ggctatggcg
     5281 atggttatcc gcgcgccgcg ccgtccggta cgccagtgct ggtgaacggt cgcgaagtac
     5341 cgattgtcgg gcgcgtggcg atggatatga tctgcgtaga cttaggtcca caggcgcagg
     5401 acaaagccgg ggatccggtc attttatggg gcgaaggttt gcccgtagaa cgtatcgctg
     5461 aaatgacgaa agtaagcgct tacgaactta ttacgcgcct gacttcaagg gtcgcgatga
     5521 aatacgtgga ttaatagttc tgtaatattt gattgtctgt gccggatgcg gcgtgaatgc
     5581 cttatccggc caataaaatc ctaaaaattc aataagttga tgttctttca tgctcttata
     5641 aaggtcgtgc ctctggcgga tgtacgtttg tcatgagtct cactctgttg ctaattgccg
     5701 ttcgctcctg aacatccact cgatcttcgc cttcttccgg tttattgtgt tttaaccacc
     5761 tgcccgtaaa cctggagaac catcgcgtgt ttcaaaaagt tgacgcctac gctggcgacc
     5821 cgattcttac gcttatggag cgttttaaag aagaccctcg cagcgacaaa gtgaatttaa
     5881 gtatcggtct gtactacaac gaagacggaa ttattccaca actgaaagcc gtggcggagg
     5941 cggaagcgcg cctgaatgcg cagcctcatg gcgcttcgct ttatttaccg atggaagggc
     6001 ttaacagcta tcgccatgcc attgcgccgc tgctgtttgg tgcggaccat ccggtactga
     6061 aacaacagcg cgtagcaacc attcaaaccc ttggcggctc cggggcattg aaagtgggcg
     6121 cggatttcct gaaacgctac ttcccggaat caggcgtctg ggtcagcgat cctacctggg
     6181 aaaaccacat agcaatattc gccggggctg gattcgaagt gagtacttac ccctggtatg
     6241 acgaagcgac taacggcgtg cgctttaatg acctgttggc gacgctgaaa acattacctg
     6301 cccgcagtat tgtgttgctg catccatgtt gccacaaccc aacgggtgcc gatctcacta
     6361 atgaccagtg ggatgcggtg attgaaattc tcaaagcccg cgagcttatt ccattcctcg
     6421 atattgccta tcaaggattt ggtgccggta tggaagagga tgcctacgct attcgcgcca
     6481 ttgccagcgc tggattaccc gctctggtga gcaattcgtt ctcgaaaatt ttctcccttt
     6541 acggcgagcg cgtcggcgga ctttctgtta tgtgtgaaga tgccgaagcc gctggccgcg
     6601 tactggggca attgaaagca acagttcgcc gcaactactc cagcccgccg aattttggtg
     6661 cgcaggtggt ggctgcagtg ctgaatgacg aggcattgaa agccagctgg ctggcggaag
     6721 tagaagagat gcgtactcgc attctggcaa tgcgtcagga actggtgaag gtattaagca
     6781 cagagatgcc agaacgcaat ttcgattatc tgcttaatca gcgcggcatg ttcagttata
     6841 ccggtttaag tgccgctcag gttgaccgac tacgtgaaga atttggtgtc tatctcatcg
     6901 ccagcggtcg catgtgtgtc gccgggttaa atacggcaaa tgtacaacgt gtggcaaagg
     6961 cgtttgctgc ggtgatgtaa tgcaggaaag caggctggag ctacccagcc tgcagtgaaa
     7021 ttaaactgtc gtcgctttca c
//
LOCUS       DAZGVQ010000221         7037 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000221 DAZGVQ010000000
VERSION     DAZGVQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.223"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..114
                     /locus_tag="ACHLAG_004780"
     CDS             <1..114
                     /locus_tag="ACHLAG_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP0657875.1"
                     /translation="TNSITEGLPREIELRQKQYEYYRDLLFSFPKPETISN"
     gene            236..3352
                     /locus_tag="ACHLAG_004781"
     CDS             236..3352
                     /locus_tag="ACHLAG_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFP0657876.1"
                     /translation="MTHQTHTIAESNNFIVLDKYIKAEQTGDSYQSESDLERELIQDL
                     RNQGYEFISVKSQSAMLANVREQLQNLNGVVFNDSEWRRFTEQYLDNPSDGILDKTRK
                     IHIDYICDFIFDDERLENIYLIDKKNLMRNKVQIIQQFEQTGSHANRYDVTILVNGLP
                     LVQIELKKRGVAIREAFNQIHRYSKESFNSENSLFKYLQLFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWAKSDNTLIKDLKDFTATFFQKHTLLNVLVNYSVFDVSQTLLVMRPYQ
                     IAATERILWKIKSSFTAKNWSKPESGGYIWHTTGSGKTLTSFKAARLATELDFIDKVF
                     FVVDRKDLDYQTMNEYKRFSPDSVNGSENTAGLKRNLDKDDNKIIVTTIQKLNNLMKA
                     ESDLPVYNQQVVFIFDECHRSQFGEAQKNLKKKFKRYYQFGFTGTPIFPENALGSETT
                     ASVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFL
                     HPMRIQEITQYILNNFRQKTHRTFPGSKGFNAMLAVSSVDAAKAYYATFKRLQEEAAN
                     KSATYKPLRVATIFSFAANEEQNAIGEISDETFDTSAMDSSAKEFLDAAIREYNSYFK
                     TNFSTDSNGFQNYYRDLAQRVKNQDIDLLIVVGMFLTGFDAPTLNTLFVDKNLRFHGL
                     MQAFSRTNRIYDATKTFGNIVTFRDLERSTIDAITLFGDKNTKNVVLEKSYAEYMEGF
                     TDAATGEAKRGFMAVVSELEQRFPDPASIESEKEKKDFVKLFGEYLRAENILQNYDEF
                     ATLKALQQIDLSDPVAVEKFKAEHYVDDEKFAELQTIRLPAERKIQDYRSAYNDIRDW
                     QRREKEAEKKEKSTTDWDDVVFEVDLLKSQEINLDYILGLIFEHNRQNKGKGEMIEEV
                     KRLIRSSLGNRAKEGLVVDFIQQTNLDDLPDKASIIEAFFTFAQREQQREAEALIKEE
                     NLNEEAAKRYIRTSLKREYATENGTELNETLPKLSPLNPQYKTKKQTVFQKIVTFIEK
                     FKGVGGQI"
     gene            complement(3417..3887)
                     /locus_tag="ACHLAG_004782"
                     /pseudo
     CDS             complement(3417..3887)
                     /locus_tag="ACHLAG_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051691.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3991..4605)
                     /locus_tag="ACHLAG_004783"
     CDS             complement(3991..4605)
                     /locus_tag="ACHLAG_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657877.1"
                     /translation="MFGMSITQSDGSLWMSPEFTPQNLINKGTMSTSKGSVFQTSIPS
                     NKSCFFFIKSSNKANMMFIHEHSNGYNALRLHQVNGSPGTITVYAFSDMVLPHSGYGI
                     AMYNSAGAMVYHGEMMPLDAKLITITDPQFTIDMGYPCAVMPAMVGVYNYRRTDYDRP
                     VYVTMTGATGNQVYNGQWYSGNVTWDIKKIYTNKILVINTSKYD"
     gene            complement(4944..5513)
                     /locus_tag="ACHLAG_004784"
     CDS             complement(4944..5513)
                     /locus_tag="ACHLAG_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657878.1"
                     /translation="MCKKCKAIADEQNALFEEMDANELVKMLAILRGIEDVSIFERVV
                     TALNFESTFEEPTQVVALAHHFGVHYLAEKERADKLQATLDMVSETQRTDDTNKSEAI
                     IASKDREIAGLKSSLTMLMSAFNLMSSQAGYKMPSLNSDDPMAVRQLLGAMADQLDDT
                     KSRLEDMMRELSHRHNLATQPHKVFQSSN"
     gene            complement(5653..5811)
                     /locus_tag="ACHLAG_004785"
     CDS             complement(5653..5811)
                     /locus_tag="ACHLAG_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP0657879.1"
                     /translation="MLERFRLVTVIALLVIAVLVDFTGKMMSVISDGVLIGLAIYFAY
                     PLVRKATC"
     gene            complement(5811..6236)
                     /locus_tag="ACHLAG_004786"
     CDS             complement(5811..6236)
                     /locus_tag="ACHLAG_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007901009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP0657880.1"
                     /translation="MLGFFKKKTRKAVIEVKKMENRDAVEATVWGGYMISYANGTCDA
                     KEISILEKTIAALPAFSPFAGEIAQMSANIRVQYEASPRRANAQALRELADVAGTDDA
                     VDVLCLCLDIADQDGIDEPEEQALKKIAQALQLSLDAYL"
     gene            complement(6330..6518)
                     /locus_tag="ACHLAG_004787"
     CDS             complement(6330..6518)
                     /locus_tag="ACHLAG_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657881.1"
                     /translation="MNWLSNHFGKIWLAILALMAAGWVSNIIKLVCSGDLQFQAGMTL
                     ARVVGIFIFPVGSVLGYF"
     gene            complement(6528..>7037)
                     /locus_tag="ACHLAG_004788"
     CDS             complement(6528..>7037)
                     /locus_tag="ACHLAG_004788"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657882.1"
                     /translation="LDGVHMARQTYFTSATKRPRSLRQILAELFSGRVMSRLDELETT
                     VRLLNERLDNQASVVANVGAIVASGSSREAKSTRPLVKEKNNKDSSNGKFSKKEAETN
                     GLRSHYSFTGDGSRSSRPEPFDAGFIHHHTSVDDNYHHSSRASCHSGWDDGGCDTSSS
                     SSYSGSCCD"
BASE COUNT         2083 a         1578 c         1505 g         1871 t
ORIGIN      
        1 accaattcca tcaccgaagg tcttccgcgt gaaatcgagt tgcgccagaa acaatacgag
       61 tactatcgtg atttactgtt cagttttccc aaacctgaaa ctatcagtaa ctaattgacc
      121 attgctaccg accggatcac cttaacatcc ggtctatata gagactattt tttacacgcc
      181 ggaagtcgcc cttaaagccc ttccggccct tgccagacgg cacaaaggat gcgctatgac
      241 tcatcagaca cacaccattg ctgaatccaa taactttatc gttcttgata agtacatcaa
      301 agccgagcaa acaggcgaca gctaccagag cgaatcggac ctggaacgtg aactgattca
      361 ggatctgcgg aatcagggct atgaatttat ttccgtaaaa tctcagtcgg cgatgctggc
      421 caacgttcgg gaacagcttc agaacctcaa tggtgtggtg tttaatgaca gcgaatggcg
      481 gcgtttcacg gagcagtatc tggataaccc gagcgatggc atcctggata agacccgtaa
      541 aatccatatc gactatattt gcgactttat ttttgatgac gagcgtcttg agaacatcta
      601 tttgatagat aaaaagaatc tcatgcgcaa caaggtgcag attatccagc agtttgaaca
      661 gacgggttct catgctaacc gttatgacgt cacgatcctg gttaatggct taccgctggt
      721 acaaatcgaa ctgaaaaagc gcggcgtggc gattcgtgag gcctttaacc agatacatcg
      781 ttacagcaaa gagagtttta acagcgaaaa ttctctgttt aagtatctgc aactgtttgt
      841 catttccaac ggcaccgata cccgttactt tgccaacacg acaaagcgcg ataaaaacag
      901 ttttgacttc accatgaatt gggcgaaatc agacaacacg ctgattaaag acctcaaaga
      961 ctttaccgcc acctttttcc agaaacatac cctgcttaat gtcttagtga actacagcgt
     1021 attcgatgtt agccaaacgc tactggtgat gcgaccgtac cagattgccg ccactgagcg
     1081 cattctgtgg aaaattaaga gttcctttac agcgaagaac tggtcaaaac cggaaagcgg
     1141 tgggtatatc tggcacacta ccggttcagg taaaaccctc accagcttta aagccgcgcg
     1201 tctggcaaca gagctggact ttattgataa agtcttcttc gtggtcgaca ggaaagacct
     1261 cgattaccag accatgaatg aatataagcg tttttcgcca gacagcgtca acggctcgga
     1321 aaataccgca ggtcttaaac gcaatctgga taaggacgat aacaaaatta tcgtcaccac
     1381 tattcagaaa cttaataacc tgatgaaagc agaaagcgac ctgcctgtat acaatcagca
     1441 agtggtgttt atatttgatg aatgtcaccg cagccagttt ggagaagcgc agaaaaacct
     1501 gaagaagaaa ttcaaacgct attatcagtt tggttttacc ggcacaccta ttttcccgga
     1561 aaacgcctta ggctcagaaa cgaccgccag cgtatttggt cgtgaattgc attcgtatgt
     1621 aattaccgat gcgattcgtg atgaaaaagt gctcaaattc aaggtggact acaacgatgt
     1681 gcggccacag tttaaatctt tagagacaga aaccgacgag aaaaaactga gtgcggctga
     1741 aaatcagcag gcatttcttc atcccatgcg tatacaggaa attacgcaat atattctgaa
     1801 taacttccgc cagaagaccc accgtacttt cccaggttcc aaaggcttta atgccatgct
     1861 ggcagtgagt agcgtggatg ccgcgaaagc ctattacgcg acgtttaaac ggttacaaga
     1921 ggaagcagct aataaatcgg ctacctataa accgctgcgt gttgcaacaa tcttctcctt
     1981 tgccgccaat gaagaacaaa atgccattgg tgaaatttcc gatgaaactt ttgataccag
     2041 cgcaatggac agcagtgcta aagagtttct tgacgctgca attcgtgagt ataacagcta
     2101 ttttaaaact aacttcagca ccgacagtaa cggttttcag aactactatc gtgatttagc
     2161 ccaacgggtt aaaaatcagg atatcgatct gttaattgtc gttgggatgt ttttaaccgg
     2221 cttcgacgct ccaacattga acacgctatt cgtcgataaa aacttgcgtt ttcacggcct
     2281 gatgcaggca ttctcccgta ccaaccgcat ttatgacgct actaaaacct tcggtaacat
     2341 cgtcactttt cgggatctgg aacgctcaac cattgatgcc ataacgctgt ttggtgataa
     2401 aaatactaaa aatgtggtgt tagaaaagag ttatgctgag tatatggaag gctttacaga
     2461 tgctgccact ggtgaagcta aacgcggctt tatggcagta gtttcagaac tggaacaacg
     2521 gttccctgac cctgccagta ttgaaagtga aaaagagaag aaagacttcg taaaactgtt
     2581 tggtgaatac ctgcgtgccg agaacatcct gcaaaactat gatgaatttg ccacgctgaa
     2641 agccctgcaa caaatcgatc ttagcgatcc tgttgcggta gaaaaattca aagcagaaca
     2701 ttatgtggat gatgaaaagt tcgctgaatt gcaaacaatt cgtctccctg ctgaacgcaa
     2761 gattcaggat tatcgttctg cctataacga tattcgcgac tggcagcgcc gtgagaaaga
     2821 agctgagaaa aaagagaaat caaccactga ctgggatgac gtagtttttg aggtcgattt
     2881 gctgaagtct caggaaataa acctggatta tatccttgga ctgattttcg aacacaacag
     2941 acaaaataaa ggcaagggcg aaatgatcga agaggtcaaa cgcttaattc gttcaagcct
     3001 ggggaaccgg gcgaaagagg gcctggtggt cgattttatt cagcaaacga acctggatga
     3061 tttaccagac aaagccagta tcattgaggc attctttacg tttgctcaac gcgaacagca
     3121 acgtgaagca gaagcattga taaaagaaga aaatctcaat gaagaggcgg cgaaacgcta
     3181 tattcgcacg tctttaaaac gcgaatacgc caccgaaaat ggcacagaat taaacgagac
     3241 attaccaaaa cttagtccgt taaacccgca atataaaacg aaaaaacaga ccgttttcca
     3301 gaaaatcgtc acgtttattg aaaagttcaa aggagtaggt gggcagatat agtgtattaa
     3361 tctgaaccag atctggcaga catctgtgca aattgcagat agctgtcaga tcaaaccatc
     3421 gtagggatgt ttgtatccac tttgaactca ggtacaccaa cgaactcttt aaccttcatc
     3481 tggtagccgg ttctgtcagt tttgatgact tcctgtttgc cctctataaa ctgctcacgc
     3541 cactcgtcgc gacgggaagc tggcatgatc agcaaaacgt tagtcatatc cgtgaccttc
     3601 ctccagtact cggcccaaat attctgtttc acccattcca gatcgacttt atccagccaa
     3661 gctcgattca aacgtgagcg ttcttcatct ggtcgatggt tcagttgcag caggcgatta
     3721 atcatgttgt ggcgttcgtc gccataaacg aagtcgtgaa cctgatacat gaaggcgatc
     3781 tctttctcgc actcggctac gatttcgtgg atgacgttca tttcctgacg ataatcgata
     3841 gcagtgtttg agttggtatc gtcgatgatg gaaagagctg tattcataat tgcaccatta
     3901 agaaaagatt tgtttaatag tgcaattatt tcaaaaatga aaaggtgcta aatagaaatt
     3961 aacaggcaat cctttaaata aaggtatttg ctaatcatac tttgaggtat ttataaccag
     4021 gattttgttt gtataaatct tcttaatatc ccatgtgaca ttaccggaat accattgacc
     4081 gttatatact tggtttccag ttgcaccggt catagttaca tagacaggtc tgtcgtaatc
     4141 ggttcgtcta tagttataaa ccccaaccat agcaggcatt actgcgcacg gataccccat
     4201 atctattgta aattgtgggt cagtaatagt gataagtttt gcatcaaggg gcatcatctc
     4261 accgtgatac accattgcgc ctgcgctgtt atacatggca atgccatagc cagaatgtgg
     4321 taacaccata tcagaaaaag cataaactgt tattgttccg gggctaccat ttacctgatg
     4381 caatctgaga gcattgtatc cgttactatg ttcatgaata aacatcatgt tcgccttatt
     4441 gctgcttttt atgaagaaaa agcatgattt gtttgatggg attgatgttt gaaaaacaga
     4501 acctttagat gtagacattg tccctttatt aatcagattt tgcggagtaa attcaggact
     4561 catccacaaa cttccatctg attgagtaat tgacatcccg aacataatta tccccagtat
     4621 gtgtatatgt aagaaccaag ccccgtatat aaatttgacc aactaaccgt atttccatta
     4681 atagtaacat ttggaacagg taaattgatg tagttgtcgt tgttaagagg cattaatgac
     4741 cacacggcat gtagtgattt acccggtggc ggattagagt acgtcttaga gcctgatgac
     4801 gccgtaaatc gatcaagaaa aaaaataggt gtaaggaagc cagtaacgtt aattccttta
     4861 ttgttgtaaa tccctgtacc gtatgccatt tttcaccctt gtttttatca acacaatatg
     4921 gccgatcatg cggccatatc agatcaatta gagctttgaa agactttgtg tggttgtgtt
     4981 gcgaggttat gtcgatggct taactcacgc atcatgtctt caaggcgact ctttgtgtcg
     5041 tcgagttggt cggccattgc tcccaaaagc tgacgaacgg ccatcggatc atcgctgttt
     5101 agtgatggca ttttataacc cgcctgagaa gacataagat tgaacgcaga catcaacatc
     5161 gttagagagg atttaagccc agcaatttca cgatctttgc tggcaataat cgcctcactc
     5221 ttgttagtgt catcagttcg ttgagtctcg ctcaccatat ccaaagtcgc ctgcaactta
     5281 tcagcgcgct ctttttcagc aagataatga acaccgaaat gatgtgctaa agctacaacc
     5341 tgagttggct cttcaaaggt ggactcaaaa ttaagtgctg taaccactct ctcaaatatg
     5401 gaaacgtctt ctattcctcg aagaatggct aacattttga ccagttcatt agcatccatt
     5461 tcttcgaata aggcgttttg ttcatcagct atcgctttgc atttcttgca catgtgtttt
     5521 tcctaatgct taaacaagtt gttttcttat tggctttatt gtgatgtggc ataaaagggg
     5581 agcaagctaa acgtaaaggt gcaaactctc tatttcggtc aacgaaacac aagggccatt
     5641 tggcccttgt cattaacacg ttgctttacg gactagcgga taagcgaagt agatcgccag
     5701 accaatgagg acgccatcag aaatgacaga catcatcttt cccgtaaaat ccaccaacac
     5761 cgctatcacc agaagagcaa tgacggtcac aagccggaat ctctcaagca ttagagataa
     5821 gcatccagtg acaactgaag cgcctgggcg atctttttca gcgcctgctc ttctggttca
     5881 tcaatgccgt cctggtcggc aatatcaagg cacagacaca gtacatctac tgcatcatca
     5941 gtcccggcca catccgccag ctcacgtaaa gcctgggcat tagcacggcg cggtgaggct
     6001 tcgtattgaa cgcggatatt ggcgctcatc tgggcaatct caccagcaaa aggagaaaat
     6061 gcaggcagag ctgcaattgt tttctcaaga atggaaattt cttttgcatc gcatgtaccg
     6121 ttggcatagg agatcatgta cccgccccac acggtggctt caaccgcatc acggttctcc
     6181 atttttttga cttcgataac agctttacga gttttctttt tgaagaaacc taacatgtgt
     6241 tttccttttg ctatttgtca aaacaaattg ttttctggtt acataaattg cttacgcaaa
     6301 gacggtcaat gcatatgcac taacaaccgt cagaaataac caagtaccga accgactgga
     6361 aaaataaaaa tcccaactac acgagccaag gtcatgccag cctgaaactg gagatcacct
     6421 gagcaaacga gttttataat gttggatacc caaccggcgg ccatgagagc cagaattgcc
     6481 agccaaattt tcccaaagtg atttgaaagc cagttcatac aaccacctta gtcacaacat
     6541 gatcctgagt aactggatga acttgaggta tcgcatccac cgtcatccca tccagagtga
     6601 caggacgctc tactggagtg gtggtaattg tcgtcgacgg aggtgtggtg atggatgaac
     6661 ccggcatcaa aaggctctgg ccggctggaa cggctaccgt cgccagtgaa actataatga
     6721 gaacgtaggc cattggtttc agcctctttc tttgaaaatt ttccattcga actgtccttg
     6781 ttgtttttct ccttcactaa aggccgtgta cttttcgctt cgcgtgaaga accagaggca
     6841 acaatcgccc ccacgttcgc aacaactgac gcttgattat ctaaacgttc attcagcaac
     6901 cgaacggtag tctctagttc atcaagacgt gacataacac gaccgctaaa caattcggcc
     6961 aaaatttgac gtaaagaacg aggacgttta gttgcagaag tgaaataggt ttgacgtgcc
     7021 atgtggactc catccag
//
LOCUS       DAZGVQ010000222         7031 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000222 DAZGVQ010000000
VERSION     DAZGVQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.224"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            577..1566
                     /gene="araF"
                     /locus_tag="ACHLAG_004789"
     CDS             577..1566
                     /gene="araF"
                     /locus_tag="ACHLAG_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFP0657883.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1636..3150
                     /gene="araG"
                     /locus_tag="ACHLAG_004790"
     CDS             1636..3150
                     /gene="araG"
                     /locus_tag="ACHLAG_004790"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFP0657884.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQLRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     PCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3165..4151
                     /gene="araH"
                     /locus_tag="ACHLAG_004791"
     CDS             3165..4151
                     /gene="araH"
                     /locus_tag="ACHLAG_004791"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFP0657885.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4318..5118
                     /gene="otsB"
                     /locus_tag="ACHLAG_004792"
     CDS             4318..5118
                     /gene="otsB"
                     /locus_tag="ACHLAG_004792"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFP0657886.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5093..6517
                     /gene="otsA"
                     /locus_tag="ACHLAG_004793"
     CDS             5093..6517
                     /gene="otsA"
                     /locus_tag="ACHLAG_004793"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFP0657887.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6524..6952)
                     /gene="uspC"
                     /locus_tag="ACHLAG_004794"
     CDS             complement(6524..6952)
                     /gene="uspC"
                     /locus_tag="ACHLAG_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFP0657888.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1774 a         1633 c         1825 g         1799 t
ORIGIN      
        1 gcaatgaatt ttaatgatag tgctttttgt atcttgttga tattaatcaa tgaatttgaa
       61 ttcatgatga agtgatgatt ttataaaacg ttttttcatt tttgcgagat ggctctcatt
      121 atacgtgttc tgttaattaa tgagcacagt gataattaat gacgaaatga atgacgtgca
      181 ttttccacat ctttgagttg cggttattac accatttcaa aaactcaacg ccaggtaatg
      241 cggcctattg actggttaaa aagaagacat cccgcatggg taccaaagac aacaaggatt
      301 tccaggctaa tcttatggat taatctgctg tgcattcgac aatttgtctg acaaattggc
      361 tttcccttat gtcttttccc gctaaattta tgcacgttct cactgtaatt ctgcgatgtg
      421 atattgctct cctatggaga attaatttct cgctaaaact atgtcaacac ggtcacttat
      481 cttttagtta aaaggtaatg ctttgttttc cgattaattt aacgaatgtc attcgttttt
      541 gccctacaca aaacgacact aaagctggag agaaccatgc acaaatttac taaagccctg
      601 gcagccattg gtctggcagc cgttatgtca caatccgcta tggcggagaa cctgaagctc
      661 ggttttctgg tgaagcaacc ggaagagccg tggttccaga ccgaatggaa gtttgccgat
      721 aaagccggga aggatttagg gtttgaggtt attaagattg ccgtgccgga tggcgaaaaa
      781 acattgaacg cgatcgacag cctggctgcc agtggcgcaa aaggtttcgt tatttgtact
      841 ccggacccca aactcggctc tgccatcgtc gcgaaagcgc gtggctacga tatgaaagtc
      901 attgccgtgg atgaccagtt tgttaacgcc aaaggtaagc caatggatac cgttccgctg
      961 gtgatgatgg cggcgactaa aattggcgaa cgtcagggcc aggaactgta taaagagatg
     1021 cagaaacgtg gctgggatgt caaagaaagc gcggtgatgg cgattaccgc caacgaactg
     1081 gataccgccc gccgccgtac tacgggatct atggatgcgc tgaaagcggc cggattcccg
     1141 gaaaaacaaa tttatcaggt acctaccaaa tctaacgaca tcccgggggc atttgacgct
     1201 gccaactcaa tgctggttca acatccggaa gttaaacatt ggctgatcgt cggtatgaac
     1261 gacagcaccg tgctgggcgg cgtacgcgcg acggaaggtc agggctttaa agcggccgat
     1321 atcatcggca ttggcattaa cggtgtggat gcggtgagcg aactgtctaa agcacaggca
     1381 accggcttct acggttccct gctgccaagc ccggacgtac atggctataa atccagcgaa
     1441 atgctttaca actgggtagc aaaagacgtt gaaccgccaa aatttaccga agttaccgac
     1501 gtggtactga tcacgcgtga caactttaaa gaagaactgg agaaaaaagg tttaggcggt
     1561 aagtaatttg ccggaaaaat tcccctctgc atgatgcaga gggggtgtga acgaccagtg
     1621 attcacggag acgttatgca acagtctacc ccgtatctct catttcgcgg catcggtaaa
     1681 acgtttcccg gcgttaaggc gctgacggat attagttttg actgctatgc cggtcaggtt
     1741 catgcgttga tgggtgaaaa tggcgcagga aaatcaactc tcttaaaaat cctcagcggc
     1801 aactatgcgc caaccacggg ttctgtagtg attaatgggc aggaaatgtc cttttccgac
     1861 acgaccgcag cacttaatgc gggtgtggcg attatttacc aggaactgca tctcgtgccg
     1921 gaaatgaccg tcgcggaaaa catctatctc ggccagctgc cgcataaagg cggcattgtg
     1981 aatcgctcat tgctgaatta tgaggcgggt ttacaactta aacatcttgg tatggatatt
     2041 gacccggaca cgccgctgaa atatctctcc attggtcagt ggcagatggt tgaaatcgcc
     2101 aaggcgctgg cgcgtaacgc taaaattatc gcctttgatg agccaaccag ctccctttct
     2161 gcccgcgaaa tcgacaatct tttccgcgtt attcgtgaac tgcgaaaaga ggggcgggtg
     2221 atcttatacg tttctcaccg tatggaagaa atatttgccc tcagcgatgc catcactgtc
     2281 tttaaagatg gacgttatgt caaaaccttt accgatatgc agcaggttga ccacgacgcg
     2341 ctggtgcagg cgatggtcgg gcgcgacatt ggcgatatct acggctggca acttcgtagt
     2401 tatggcgagg agcgcctgcg tcttgatgct gtgaaagcac caggcgtgcg tacgccaata
     2461 agtctggcgg ttcgcagtgg tgaaattgtt gggctgtttg gtctggtagg agcggggcgt
     2521 agcgaattaa tgaaaggctt gtttggcggg acgcaaatca ccgccggtca ggtttatatc
     2581 gaccaacagc cgatcgatat tcgtaaaccg agccacgcca ttgccgcagg catgatgccc
     2641 tgcccggaag atcgcaaagc ggaaggcatt attcccgtgc actccgttcg cgacaatatc
     2701 aacatcagtg ccagacgtaa acatgtgctc ggcggttgtg taatcaacaa cggttgggaa
     2761 gaaaacaatg ccgatcacca cattcgttcg ctcaacatca aaacgccggg cgctgagcaa
     2821 ctgatcatga atctctcagg cggaaatcag caaaaagcca ttctgggccg ctggttatcg
     2881 gaagagatga aggtcatttt gctggatgaa cctacgcgcg gcattgatgt tggtgctaag
     2941 catgaaattt acaacgtgat ttatgcgctg gcggcgcagg gtgtggcggt gctgtttgcc
     3001 tccagcgact tacctgaagt cctcggcgtt gccgaccgga ttgtggtgat gcgggaaggt
     3061 gaaatcgccg gtgaattgtt acacgagcag gcagatgagc gtcaggcact gagccttgcg
     3121 atgcctaaag tcagccaggc agttgcctga gtaaggagag tatgatgtct tctgtttcta
     3181 catcggggtc tggcgcacct aagtcgtcat tcagcttcgg gcgtatctgg gatcagtacg
     3241 gcatgctggt ggtgtttgcg gtgctcttta tcgcctgtgc catttttgtc ccaaattttg
     3301 ccaccttcat taatatgaaa gggttgggcc tggcaatttc catgtcgggg atggtggcgt
     3361 gtggcatgtt gttctgcctt gcttccggtg actttgacct ttctgtcgcc tccgtaattg
     3421 cctgtgcggg tgtcaccacg gcggtggtta tcaacctgac tgaaagcctg tggattggcg
     3481 tggcagcggg gttgctgctt ggcattctct gtggcctggt caatggcttt gttatcgcca
     3541 aactgaaaat aaatgctctg atcacaacac tggcaacgat gcagattgtt cgaggtctgg
     3601 cgtacatcat ttcagacggt aaagcggtcg gtatcgaaga tgaaagcttc tttgcccttg
     3661 gttacgctaa ctggttcggt ctgcctgcgc caatctggct caccgtcgcg tgtctgatta
     3721 tctttggttt gttgctgaat aaaaccacct ttggtcgtaa caccctggcg attggcggga
     3781 acgaagaggc tgcgcgtctg gcgggtgtac cggttgttcg caccaaaatt attatctttg
     3841 ttctctctgg cctggtatct gcgatagccg gaattattct ggcttcacgt atgactagtg
     3901 ggcagccaat gacgtcgatt ggttatgagc ttattgttat ctccgcctgc gttttaggtg
     3961 gcgtttctct gaaaggtggc atcggaaaaa tctcatatgt ggtggcgggt atcttaattt
     4021 taggcaccgt ggaaaacgcc atgaacctgc ttaatatttc tcctttcgcg cagtacgtgg
     4081 ttcgcggctt aatcctgctg gcagcggtga tcttcgaccg ttacaagcaa aaagcgaaac
     4141 gcactgtctg atgctttttt ccacaacaat ttaacgtttt ttcccaccac agccagccgc
     4201 cacaacggtt ggctgttctt cattgcaaat ggcgaccccc gtcacactgt ctatacttac
     4261 atgtctgtaa agcgcgttct gcgcaaaaca ataagaaaag agaaggagga gagccgggtg
     4321 acagaaccgt taaccgaaac ccctgaacta tccgcgaaat atgcctggtt ttttgatctt
     4381 gatggaacgc tggcggaaat caaaccgcat cccgatcagg tcgtcgtgcc tgacaatatt
     4441 ctgcaaggac tacagctact ggcaaccgca agtgatggtg cattggcatt gatatcaggg
     4501 cgctcaatgg tggagcttga cgcactggca aaaccttatc gcttcccgtt agcgggcgtg
     4561 catggggcgg agcgccgtga catcaatggt aaaacacata tcgttcatct gccggatgcg
     4621 attgcgcgtg atattagcgt gcaactgcat acagtcatcg ctcagtatcc cggcgcggag
     4681 ctggaggcga aagggatggc ttttgcgctg cattatcgtc aggctccgca gcatgaagac
     4741 gcattaatga cattagcgca acgtattact cagatctggc cacaaatggc gttacagcag
     4801 ggaaagtgtg ttgtcgagat caaaccgaga ggtaccagta aaggtgaggc aattgcagct
     4861 tttatgcagg aagctccctt tatcgggcga acgcccgtat ttctgggcga tgatttaacc
     4921 gatgaatctg gcttcgcagt cgttaaccga ctgggcggaa tgtcagtaaa aattggcaca
     4981 ggtgcaactc aggcatcatg gcgactggcg ggtgtgccgg atgtctggag ctggcttgaa
     5041 atgataacca ccgcattaca acaaaaaaga gaaaataaca ggagtgatga ctatgagtcg
     5101 tttagtcgta gtatctaacc ggattgcacc accagacgag cacgccgcca gtgccggtgg
     5161 ccttgccgtt ggcatactgg gggcactgaa agccgcaggc ggactgtggt ttggctggag
     5221 tggtgaaaca gggaatgagg atcagccgct aaaaaaggtg aaaaaaggta acattacgtg
     5281 ggcctctttt aacctcagcg aacaggacct tgacgaatac tacaaccaat tctccaatgc
     5341 cgttctctgg cccgcttttc attatcggct cgatctggtg caatttcagc gtcctgcctg
     5401 ggacggctat ctacgcgtaa atgcgttgct ggcagataaa ttactgccgc tgttgcaaga
     5461 cgatgacatt atctggatcc acgattatca cctgttgcca tttgcgcatg aattacgcaa
     5521 acggggagtg aataatcgca ttggtttctt tctgcatatt cctttcccga caccggaaat
     5581 cttcaacgcg ctgccgacat atgacacctt gcttgaacag ctttgtgatt atgatttgct
     5641 gggtttccag acagaaaacg atcgtctggc gttcctggat tgtctttcta acctgacccg
     5701 cgtcacgaca cgtagcgcaa aaagccatac agcctggggc aaagcatttc gaacagaagt
     5761 ctacccgatc ggcattgaac cgaaagaaat agccaaacag gctgccgggc cactgccgcc
     5821 aaaactggcg caacttaaag cggaactgaa aaacgtacaa aatatctttt ctgtcgaacg
     5881 gctggattat tccaaaggtt tgccagagcg ttttctcgcc tatgaagcgt tgctggaaaa
     5941 atatccgcag catcatggta aaattcgtta tacccagatt gcaccaacgt cgcgtggtga
     6001 tgtgcaagcc tatcaggata ttcgtcatca gctcgaaaat gaagctggac gaattaatgg
     6061 taaatacggg caattaggct ggacgccgct ttattatttg aatcagcatt ttgaccgtaa
     6121 attactgatg aaaatattcc gctactctga cgtgggctta gtgacgccac tgcgtgacgg
     6181 gatgaacctg gtagcaaaag agtatgttgc tgctcaggac ccagccaacc cgggcgttct
     6241 tgttctttcg caatttgcgg gagcggcaaa cgagttaacg tcggcgttaa ttgttaaccc
     6301 ctacgatcgt gacgaagttg cagctgcgct ggatcgtgca ttgactatgt cgctggcgga
     6361 acgtatttcc cgtcatgcag aaatgctgga cgttatcgtg aaaaacgata ttaaccactg
     6421 gcaggagtgc ttcattagcg acctaaagca gatagttccg cgaagcgcgg aaagccagca
     6481 gcgcgataaa gttgctacct ttccaaagct tgcgtaggag cggttaatca cccgtaagtg
     6541 gaactaacag cacatcgacc tcacttgagg caatcactct tttcgccgag caggatgctc
     6601 gcgaaaagaa actgtgattg tgattaccgc aaataaccaa atcgaaatga tgtttgtgac
     6661 atacttccag aatatgttcg cttaactcac cgtaggcaat aaacgtcttg tcaacgggat
     6721 accccgcatc ctgaattaac ttatcaagaa agctttgcgt ttcttcttgc atcacgctgc
     6781 gtaaatcttc aagcatcggc gcggctaatt gattgtacat ttccgggtcg gaagcgagag
     6841 taattaaact gatgtgtccc tttactggcc tggcgataga taccgctttt gccagcagtt
     6901 gctgactttc cggtgtaacc gcaacagcga caagaatatt gctatagctc ataacctgtt
     6961 ccttattctg tgaacttcag gtgacattaa agctttactc ctaatggaga aacgacgcaa
     7021 tcccaactcg g
//
LOCUS       DAZGVQ010000223         7031 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000223 DAZGVQ010000000
VERSION     DAZGVQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.225"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(73..2856)
                     /gene="pqqL"
                     /locus_tag="ACHLAG_004795"
     CDS             complement(73..2856)
                     /gene="pqqL"
                     /locus_tag="ACHLAG_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP0657889.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2913..5287)
                     /gene="yddB"
                     /locus_tag="ACHLAG_004796"
                     /pseudo
     CDS             complement(2913..5287)
                     /gene="yddB"
                     /locus_tag="ACHLAG_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
     gene            complement(5325..7010)
                     /gene="yddA"
                     /locus_tag="ACHLAG_004797"
     CDS             complement(5325..7010)
                     /gene="yddA"
                     /locus_tag="ACHLAG_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFP0657890.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     IAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVPP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEILHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
BASE COUNT         1824 a         1610 c         1582 g         2015 t
ORIGIN      
        1 ttataaaatt attacataaa aatagcgaat attgctaaaa tccccgccaa cgatgtgttg
       61 acggggctgt tattattttg gcaataatac tccggtataa gtatttaccg gatgagaaag
      121 atattgttta acggcagtgt taacattctc taccgtcatt tgtttcaaca attgctcctg
      181 ctcagtccat gctgcaggat cgtcatattg aataagacta tttacaatag tgttcgctaa
      241 ttgttgaacg ctacgctgtt ggatatcgag gctgcgctga acgttttgct ggtattcatt
      301 cagttcttgc tcactgatcc ctttagccag acgcttaacc atcacttcat tcgctaacgt
      361 taacagttca tcatgtcgtt ctggttgaca agtaaaagcc agcaaatgac tgatatcttt
      421 ggcctgagga tcaaccgaga ggcgagaaga aacgctgtat gctccagatg cctgttcacg
      481 aatattaaca cgtagatctt ttgccagtgc gacgttaaaa gcatcgagcg ccatacgcgt
      541 cgccagatta acaggtgtcc gggaatcata acgcttccac tgtgaaacct gtgccacagg
      601 ttcattttgt tcttttacag taaccgatgc gttgtccgtc gcgcgagtta atggtttacc
      661 tgcggctaat ggcgaatcag agtgtttgat tgatcctaag taacgcgtaa ttaacgccac
      721 gagtttgtct tctgagacat taccgacaat gacaaacgtg atatccgctg gagatgaaaa
      781 caactggcga tcggcagcca gcgcatctgc ggcagtaaac tgtacaatct gattttcttg
      841 cagtaatttc gtgcggtcat cagcatagcg cgtctcatac atctgctggg cgaatttctc
      901 cgctggacgc tggtcgagcg ttttcaacgc ctgaatttga gcattttgta gcgatgccca
      961 aatattgtcg ttaatcgtgc tgtgggtgat tcgctggtta atcaactgaa aaccaggttc
     1021 agggttatta gtccgcgcgc taacggagag caacgtattc atgccactga ctttactgct
     1081 catggttacc gaattttccg cactccagcg tttcaggctg gaagaggaga gctcaccaac
     1141 gccgcttccg cttactgctt tatttgccag cgcgataagt gatttttgct gcgcagggaa
     1201 acttaaatcg cctttattcg atacggcagt aatttgtagc ttttgctctt cacccgcgga
     1261 ttttgccaga ataaccctgg caccattgga aagtgttaat gatgtcagat tctcagccag
     1321 cgtttctttg ctgctaattt ccgcctgtgg gtcagcgtct actgttaacg ataaatttct
     1381 gcctgggaag atgtaagccg ccagcttttt gttggcgtac tctttttcca gcgccaggat
     1441 agccgcagga gacaatgctt ttttggcggc aagctcattg tttaccattt gctcccaaaa
     1501 tgcgtcctgg ttctttctta actgctgcca tttttccgcc agactttgca cggtaatttg
     1561 ctgccacaga cgtttcgaaa gttgatatgt ctcttccggc gacaagaacg gcgtattatt
     1621 taatgagctg gatgccaggc gactggtcag catacgtaaa tcacgttcag cttgctgatc
     1681 aaccgcattt ttcagccagg tgaggcgggt agatttgaca tcatcgagtt cttcagcaga
     1741 aaaaccatgc tgatcaatgg ttgccaactc tgccattaat gcattcgcag catcctgcat
     1801 attatcgtct cgtgcattta cacggaaaaa cagcgactga taatcgggtg caattttaac
     1861 gctgcgcgca gtgccgccag aaatagtctt caactcgccc gactgtatgc gttcctgcag
     1921 acgttgattg aacagctgaa ctaacatgct ccattcagct tgttcgataa agctttgctc
     1981 atcgttcact tgtaccattg gcaggcgata atagagtgcg atgccgttca cccggttttc
     2041 tttatcatta atgatattaa agcgcaggtg gttttcggct tttgtcggcc agacgcgatt
     2101 ttcagctgct ttgttagccg gaagcttact taaattatcc tttatcagcg ccagcgcttc
     2161 tttactgtcg atatcgccga ccacgataaa ggtcatatta tttggttgat accagcgttg
     2221 ataaaattgg cgcaattgtg ccggtgtgac cgtggcgaca gtatccatca ggccgatagg
     2281 ttcacggtct aaattacggg tattagccag caggaaaggg cggcgcgcct gagaggtgcg
     2341 ccatttcgca tcctgatggg cacgccattc ctcagtaatt acgccacgtt cagcgtctac
     2401 ttcgagtttt tcaaaggttg cggcattact ccattcactg aagattgcca tcacttgttg
     2461 cagattttgt ttctgcgtag tcggcaaact cacctgatac accgtttcgt catagctggt
     2521 ataggcatta acatcgcgac caaaacgcag gcccattgac tcaaatgttt cgatgacttt
     2581 attacccggc catgtttttg tgccgttaaa catcatatgt tctacaaaat gagccacgcc
     2641 gcgctcattg tcttcttcct gcaatgaacc ggtatgaatt tgcagccata aatttacctg
     2701 atcctttgga tgagcatgcg gataaatcat atatcgcaag ccattgccca gttgcccggt
     2761 aattaacttt tcatcctgcg gtaaggcggc ggcaatcagc cgcccgggga gcaacagagt
     2821 tgccactaac gtcagtaaga aacagaggtt tctcataatt atctccatgc gaaaaccggg
     2881 cgaatttacc cggttaagta aaatccgaac tattaaaatt tcatgctgac atcaagccag
     2941 aaagtacggc cactggcgta ggtcgccaca ccggtattcg ttgtatcaac agcggttttg
     3001 ctatccaata cattgagaat atcggcactg atcgtcaggt tttgttgttt cagaaattgt
     3061 ggcgtccagg acaagcgggt gtcccaggtc aggctgctat ccagtttttc gtcaacatac
     3121 tgcttgtaat ctgaatattc gctgatgtat tgcgcatttg tcttacccag gataatgcga
     3181 gctttacgcg cttcttgcca ggccagcgta ttcgcccaca ccaacccgct cggttgatgc
     3241 gtgaaatcca tgtttaagga gatctttaat gggttattaa aatctgccac tggaacgcta
     3301 tcgtaagaga caagattacc gttataaacc acctggttat caccggtatt gctctcctca
     3361 taaccattgt ttaacgacaa gttgcccttg ctcttgatat agctaaagac aatttgtggg
     3421 ttaatatcta cctggcggat atgcaggggt tcggccagct caaaactgag gttgaacgaa
     3481 tgcgttttgg ttttgccatc gttgttatat tcagtaatgg tggttttagt cgcgctgtcg
     3541 gtacgactgc ttttgctgat ttgatcatgc gcttcacggt aaacatagtt tgcacgcgca
     3601 ataacgttct taccgatttt ctgctgcaat cccattgcca gttcatcgtt ataaggcgtt
     3661 ttcaaatcct gataacgcgt cagggtttta ttgcctgata ccgattccgt ccagctattg
     3721 cggatatcac gtaatcccat atcaagaata ttcccaccat agtaacggtt ataacctgcg
     3781 gtaatcattg aggtttgatc agcaaaaata tcccattccg tcataaagcg cggggagata
     3841 ttgtggtttg acagatagtt gtcatagtca taccgcacgc cgggcattaa tgacacatta
     3901 cgccagctaa tgcgatccgc catatacagt gtgtagttgt caattcccag gctgccttta
     3961 cctttatggt aaatggtatg gttagttttc tttccggcag cattaatcac ataggattca
     4021 gactggttat ggcgttcagt ccatgcatcg gaatagatgt attccgcgcc gaagtagggt
     4081 tgatgcgaaa cattaccacc gcggcgaatt tttgccagtc caggcgtgtt ttgaaggtgt
     4141 aattatctac agcctgggaa atgtgtccta atccgccacg ggtgcaacgc cctgtaatat
     4201 caccatatgt acatgaaagt tcggtgtacc aggtgtcatg atcgtgacgg gtataatcac
     4261 taatatgatc ccaaccaacg gtggtacgaa gtttggccca tgcgagctgc gtatccatat
     4321 cccatgccag accataggat ttattaccca tttcgcgatc agactgcggg aaggtgctgg
     4381 tattataatc acggctggag ccggtatatt ttaaggttaa atcgtgggta aagcggtcgc
     4441 tggcaaacca ggtaaatttg ctcaatgcag tatcgataac gtttttatac tgtgcccgac
     4501 cggcgacaat gccgtcattc gaaacataat ccgcgcgggt gatatcagac tggcggcgcg
     4561 ataaaccggc ggtaacgcca aagttatcag cgagttcctg attaaacgac aaggtataaa
     4621 agttcttttt aaaatctgga gagtaataag tacttcctga agaaccttga ttaaatgcgc
     4681 tcttgttatt ctcatcgata tgcgatgtta accagtccga acgcgtagtg cgataaccca
     4741 gtttcacgct gctatcatca gcgttgaagc gtttgatctt tgcatcaatt accccgccat
     4801 tgaagcgacc gaattcaacc ggcacaaaac tgtcataaag cgtcacattg tccagtaagc
     4861 tgacatcaag ataatacccc tgtgacatcc cgctaatatt ggttgcacta ctggcatcgg
     4921 actcattcgc tgggttcagg ttattagtgg cactaatacc atcaatcaaa taggcattct
     4981 ggtagggcga cgcaccgtga atagagattt tctccgggcg aatatctccc tggttcaacg
     5041 aggtactttg cgttgaatcc atgcgtaccg cagggttggt tctcagcaga tcgctgatat
     5101 tgccattccc ggtcggtaaa cgctcgatgc tttcactggt gtaatgcgtg ttgccgttta
     5161 cgggtacagg gacaggagca tagaccgtca tctcttcaaa aacatgcatg tacatgtttt
     5221 tgtcatcgac ggcctgcgcc acatcagcgc cacataaaat gacgccagga ataagaactc
     5281 gcttcattta tttatatctc cgtacagcag ggcttttatt gtttttataa aaccgcgcta
     5341 atgtcacaaa tatcatcggc caggttccag acaccgggtt gatgtgtaac cataatgacg
     5401 ccgcttgtgg gtagtttttc acgcactaaa cgcagtaagc ggatagcctc ttgttcctca
     5461 agatgagagg tagtttcgtc aagaaatatc catttcggac gtcgtaaaat taatcgtgcc
     5521 agggcgatac gttgtttttc gccgctggaa agaatatctc cccagcgatc gtggtcatga
     5581 atacgcgcag ccaatttccc cagaccaacc tgatgcagta tttcgctcaa cgatttatcg
     5641 tctacgggca gaggaagtgc tttacaaata atctctttca gtaagccggt tttgattaac
     5701 ggtgtttgtg acacatacca actgtcggca ggagaagaaa tatcaccttt aaaccacggc
     5761 cagcagtggg ataatgtttt aagcagtgtg gtttttcccg caccagagta acctttcagt
     5821 aatagccatt tgcctggcgg aacatgaaag ttcaggttct ctaatatgat cttattatca
     5881 ggggtacgaa tactcgcgtc agccacttgt accgcatgtt ggcaattttt aggcttattc
     5941 gtagggcgct gttcggtgag ttgatggaac tcatacaagc gatcgataac cgcagccagt
     6001 tcagcaattt catcatattt ataaataaac cagcttaaat tgttcgatac cagcataaat
     6061 gcctggcgcg atttcataag tccgcccaga ttgatctgtc cgctaataaa ctgcggcaat
     6121 aacagaaagt acggaagaac gctaagcgaa cgcgaataaa tattctgcca gtaatcaagc
     6181 caccgctgac gattcattaa acgatgccag ttctctttaa tggtgtgaaa attgtcgctc
     6241 aactcctggc gttgtagaga ctcagcattg cttagcgcaa tcagttctgc ctgtttatta
     6301 tgctgcacaa gattagttcg aaatgtcgct tcgctacgtt gtttttccac attaagcgga
     6361 cgaatacgtt taccaacctt atgggtaaat aaagttccac cgatcacaat gagcacaacg
     6421 gtatagacca tatacccctg gatattccat tctgttccac caacagtgaa tgagagcgta
     6481 cccgcgcttt gccaaagaat aacggtaaag gtgatcagca tgctaagtga ctggatgaag
     6541 ccaaatgaaa ggctgagcgt tttgctgatc agtaagagaa tgtcttcagc gatacgttgg
     6601 tcagggttat ctgtattttt atgctcgccg tagatctgcg tgaagtagta gtttttatct
     6661 gcaaaccacc gattaaggta gtaatctgtt aaccattcgc gccagcgaat agtcagtagt
     6721 ttaattaacc atgttttgtt tacggatatt aatacaaaga tccccagcag ggcagggaac
     6781 caaaggacaa gctgccaaag cttatcggtt tctttctggc ttagcgcatt gaaaaaatca
     6841 ttattccagt cgtttaacca gacctgaatt ttaaccacgc caaggatcat ggcgaggata
     6901 ataatgatta acaacactga agttttatta ttcttacgca gccaaaaggg cttcagcaga
     6961 cagagatact ttgctatcaa catacaaaac gtaatgggaa tggttatcat taacgaaaat
     7021 tgattgtgcc a
//
LOCUS       DAZGVQ010000224         6950 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000224 DAZGVQ010000000
VERSION     DAZGVQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.226"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            59..556
                     /gene="ftnA"
                     /locus_tag="ACHLAG_004798"
     CDS             59..556
                     /gene="ftnA"
                     /locus_tag="ACHLAG_004798"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFP0657891.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(594..833)
                     /gene="yecH"
                     /locus_tag="ACHLAG_004799"
     CDS             complement(594..833)
                     /gene="yecH"
                     /locus_tag="ACHLAG_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFP0657892.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            1024..2235
                     /gene="tyrP"
                     /locus_tag="ACHLAG_004800"
     CDS             1024..2235
                     /gene="tyrP"
                     /locus_tag="ACHLAG_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFP0657893.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2297..2962)
                     /gene="yecA"
                     /locus_tag="ACHLAG_004801"
     CDS             complement(2297..2962)
                     /gene="yecA"
                     /locus_tag="ACHLAG_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFP0657894.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3158..3244)
                     /locus_tag="ACHLAG_004802"
     tRNA            complement(3158..3244)
                     /locus_tag="ACHLAG_004802"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3208..3210),aa:Leu,seq:taa)
     gene            complement(3257..3330)
                     /locus_tag="ACHLAG_004803"
     tRNA            complement(3257..3330)
                     /locus_tag="ACHLAG_004803"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3296..3298),aa:Cys,seq:gca)
     gene            complement(3384..3459)
                     /locus_tag="ACHLAG_004804"
     tRNA            complement(3384..3459)
                     /locus_tag="ACHLAG_004804"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3424..3426),aa:Gly,seq:gcc)
     gene            complement(3611..4159)
                     /gene="pgsA"
                     /locus_tag="ACHLAG_004805"
     CDS             complement(3611..4159)
                     /gene="pgsA"
                     /locus_tag="ACHLAG_004805"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFP0657895.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLEQ"
     gene            complement(4216..6048)
                     /gene="uvrC"
                     /locus_tag="ACHLAG_004806"
     CDS             complement(4216..6048)
                     /gene="uvrC"
                     /locus_tag="ACHLAG_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFP0657896.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6045..6701)
                     /gene="uvrY"
                     /locus_tag="ACHLAG_004807"
     CDS             complement(6045..6701)
                     /gene="uvrY"
                     /locus_tag="ACHLAG_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFP0657897.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6843..6914
                     /locus_tag="ACHLAG_004808"
                     /pseudo
     CDS             6843..6914
                     /locus_tag="ACHLAG_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT         1678 a         1805 c         1653 g         1814 t
ORIGIN      
        1 aaaataacaa attatagtga cgccacagca acaaatataa cctttgtgga gcactatcat
       61 gctgaaacca gaaatgattg aaaaacttaa tgagcagatg aacctggaac tgtactcttc
      121 actgctttat cagcaaatga gcgcctggtg cagctatcat accttcgaag gtgctgccgc
      181 gttcctgcgc cgtcacgccc aggaagagat gacgcatatg cagcgtctgt ttgattacct
      241 gactgatacc ggcaatttac cgcgtattaa taccgttgaa tctccgtttg ctgaatattc
      301 ctcacttgat gaattattcc aggaaaccta taaacacgaa caattaatca cccagaaaat
      361 taacgaactg gctcatgctg caatgaccaa tcaggactac ccaacattta atttcctgca
      421 atggtatgtt tctgagcagc atgaagaaga gaaactgttc aaatcgatta ttgataaatt
      481 aagcctggca ggcaaaagcg gcgaaggtct gtattttatc gacaaagaac tctctaccct
      541 cgacacacaa aactaatgct ctccggcggc aggtttactg ccgccgtttc aaatcagtga
      601 cggcaaatct tactctgatc ggtcgaaaaa ccgtctttcg aaggtataaa tttgcctttt
      661 gctgccagaa acgctaccag ctctcccgct gtcatccctt ctgccgagca ggtgtgaaaa
      721 cgtgcctgtt caccaaaacg cgctttaatc gcagcttcca gactggcatg cgtatattgc
      781 tcgcctgatt caatcatcat atttaacact tcatgaccgt gaatagagtc catcgtccct
      841 cctcaaaaaa agcctagcgt agcgattgcc gcttatgaag actttgcgcc agcgcaggac
      901 tgaatgcttt ttattgtaca tttatattta caccatatgt aacgtcggtt tgacgaagca
      961 gccgttatgc cttaacctgc gccgcagata tcactcataa agatcgtcag gacagaagaa
     1021 agcgtgaaaa acagaaccct gggaagtgtt tttatcgtgg cgggaaccac aattggcgca
     1081 ggcatgctgg caatgccgct ggctgcggcc ggtgttggtt ttagcgttac gttaatcttg
     1141 ttgattgggc tttgggcgtt gatgtgctac acggcgctat tactgctgga ggtgtaccag
     1201 catgttccgg cagataccgg tctgggcacg ctggcaaaac gctatctggg acgctacggt
     1261 caatggctga cgggcttcag tatgatgttc ttaatgtatg ctctgactgc ggcatacatc
     1321 agcggtgccg gtgaattgtt ggcctccagc atcagcgact ggacaggtat ttcaatgtcg
     1381 gcaaccgctg gcgtgctgtt gttcactttt gttgccggtg gcgtggtttg tgtcggaact
     1441 tcactggtcg atttatttaa ccgttttctg ttcagcgcca agattatttt tctggtggta
     1501 atgctggtac tactgctgcc gcatattcac aaagtgaatc ttttaaccct gccgttgcaa
     1561 caggggctgg ctctgtctgc aatcccggtg atttttacct catttggttt tcacggtagc
     1621 gtgccgagta ttgtcagcta tatggatggc aacgttcgta agctacgctg ggtgtttata
     1681 accggtagtg cgatccccct ggtggcatat attttctggc aggtggcaac gcttggcagc
     1741 attgattcaa caacctttat gggattgctg gctaatcatg ccggattaaa cgggctgtta
     1801 caggcattac gcgaaatggt ggcctctccg catgttgagc tggcagtgca tttatttgct
     1861 gatttagccc tcgccacgtc atttctcggc gttgcgttag gcttatttga ttatctggct
     1921 gatttatttc agcgttcaaa taccgttggt ggacggttgc aaactggtgc aattaccttt
     1981 ctgccgccgt tggcgtttgc actgttttat ccacgaggat ttgtgatggc gctgggttac
     2041 gccggtgtgg cgctggcggt actggcattg attatccctt cgctgttgac ctggcaaagc
     2101 agaaagcaca atcctcaggc gggttaccgg gtgaaaggtg gtcgtccggc gctggtggtg
     2161 gtgtttctct gtggtattgc tgtgattggc gtgcaatttt tgattgcggc agggttgtta
     2221 ccagaagtgg ggtgatcaga tagcctcaaa ttccttattg ggtgccagaa ttaacgctga
     2281 cacccaattt gacctcttaa tgcaggcagc actgcttaaa tttcttacca ctaccgcacg
     2341 ggcaaggatc gttacgcccc ggtttctctt ctgctttgat cggttgctga acagcttttt
     2401 cctgcggatg cgccatccag tacgcatgta gatcaagcgc cgccagtcga atggcatcta
     2461 cgctctcttc aaacgcttct ggcgacattt tctctacccg ctcgaagttt tcctcagtac
     2521 cgtgcagcgc aatcgcctcc agcgctggtt ttaacgaatc gggcaacgtt gaccagtcag
     2581 aaagtgccac gccccgcata tagccaaagc accactcctc aacaatcgtc agctcgctgc
     2641 catcaacttc tcgcaagccg aataacggct caaactgctc cgggaattcg ttcagacgct
     2701 ctgcggtatc ggccatatgt tgaaaagcca gattcataaa gcgcgtcatc tctttctctg
     2761 acgcccagcg cggcacatag tcagccccac cccacacggc aaccagccac tgttccggtt
     2821 caatctcttg cggagaactc aacaccgccg tcaataaacc gtccagctcc gccacatcaa
     2881 ggatggcgtg gtcagtgttg tatttggtca gaatatcgtc cagccattcc aactcacttt
     2941 cgtttaacgg tcccgttttc atacgctttt ccttgtggat ctcaactcgc cagcacctat
     3001 cttacatgcc ggtccgtatc agagatactt tttgagtggc tttgctggtg attaaaaatt
     3061 aaggagggtg taacgacaag ttgcaggcac aaaaaaacca cccgaaggtg gtttcacgac
     3121 actgcttatt gctttgattt tattcttatc tttcccatgg tacccggagc gggacttgaa
     3181 cccgcacagc gcgaacgccg agggatttta aatcccttgt gtctaccgat tccaccatcc
     3241 gggctcggga agaaagtgga ggcgcgttcc ggagtcgaac cggactagac ggatttgcaa
     3301 tccgctacat aaccgctttg ttaacgcgcc aaattcttca ggcctttcag ccagacatcc
     3361 gctgacgccg atgtctttta aactggagcg ggaaacgaga ctcgaactcg cgaccccgac
     3421 cttggcaagg tcgtgctcta ccaactgagc tattcccgca ttcatcaagc aatcagttaa
     3481 tcacttgatt ttattatcgt ctggcaatca gtgccgccgt tcgatgcgtt gcattctact
     3541 tacctggcgc gatgagtcaa cgatattttt caccactttt gattgtttgc tgaaaattac
     3601 gccgaaacga tcactgttca agcaaatctg cacgcgcagc gctcaaatat tgcaacattg
     3661 accacagagt cagtaccgca gccacaaaga aaagtgcaat accggcgtac tcaacccaaa
     3721 tgttcggacg ccacagcagc caggccaacg ccaccatctg ggcagtggtt ttcactttcc
     3781 caatccagga gacagccacg ctactgcgtt tacccaactc cgccatccat tcgcgtagcg
     3841 cagaaataat aatttcacgg gcgatcatcg ttgccgccgg taaggtcacc caccagctgt
     3901 gataatgctc ggttaccagc accatggcga tagccacgag aactttatct gcgacagggt
     3961 caaggaaagc accaaaccgg gtactctggt tccagcggcg tgccagaaaa ccatcgaacc
     4021 agtcagtcac cgccgcgacg cagaaaatga gcgcggcggc aaacggcgac caggtgacag
     4081 gcagataaaa gaccaataca aagaatggga taaggatgac acggaacagt gtaagcaacg
     4141 tagggatatt aaattgcata atgacgggta actatctgtt gtcagtaaga ttacccctat
     4201 gttgctacag agacatcaat gtttcaacga ccagaagatc ttttctgcca gaccttgcga
     4261 aatacccggc acttttgcaa tttcctcgac gctggcgtta cgtaaacctt gcaaaccgcc
     4321 catatatttc aacaacattt gccggcgttt tggcccgacg ccttcaatgg tttccaggga
     4381 actggtattt ttgaccttcg cccgtttttt acggtgcccg ccaatcgcgt gatcatgtga
     4441 ttcatcgcga atatgctgga taacatgcag cgcgggagaa tctggcggca aactgaatcc
     4501 ctcaccttcc ggctcaaaga acagcgtttc cagcccagcc ttacgatctg ctcctttggc
     4561 aacgccaagt agcagcggat gatttttatc ccatgagaca tccagttcgg cgaagacatt
     4621 tttcgcctgc gcaagctggc ctttgccgcc gtcgataagt atcacatccg ggatcttact
     4681 gtcgtcgatg gctttaccat aacgccgacg cagcacctga ttcatcgccg cataatcatc
     4741 gcccggcgtg atgccagtaa tgttatagcg ccgatactcc gcacgcagcg ggccgttagc
     4801 atcaaacacc acacaggaag cgacggtttg ttcgcccatg gtatggctga tgtcaaagca
     4861 ctccatccgc ttcacttccg gcaatttcaa cacgctggca agcgcggtca gtcgctggtg
     4921 aacggtagat tgctgcgaaa gtttgctggt taaggccgtc gccgcattgg tgcgcgcgag
     4981 tttcagataa cgcgccctat cgccgcgagg tttggtttga acattaatct tgcgtcccgc
     5041 cagttctgaa agggaatcgg cgagcagcgt tttatcgcta agattaaaat cgagcaggat
     5101 ctcacccggt aaggtgcgca tctggctgcc ttgtaaatag aactggccta cgaaggtttc
     5161 taccacctcg ctcagttccg taccgccagg cactttcggg aaatagctgc ggctgccgag
     5221 cactttgccc tgacgaatga acaatacgtg gacacaagcc atgcccgcat cgaacgccac
     5281 accaataacg tcgaggtcgt cgccggtatt ggaaacaaac tgtttttcgg tgacgcgtcg
     5341 caccgcctga atttggtcgc gaatacgtgc agcttcttca aactccagat tctggctggc
     5401 agtttccata cgactaatga gttgcgtaag cacctgatca tctttgccag acaaaaacag
     5461 gcgcacatac tcgacctgct gagcgtattc ttcttcactc accagtcctt caacgcacgg
     5521 tcccagacag cgccctatct ggtattgcag acacggacgc gagcgattgc gataaacact
     5581 attttcgcac tggcgaatgg ggaaaatctt ttgtagtagc gccagtgttt cacgtacggc
     5641 atagccattc gggaacgggc cgaaatattc acctttggca tgcttcgcac cacgatgcat
     5701 cgccagacgc gggtgggtat caccactcag gaagataaaa ggatatgatt tatcatcgcg
     5761 tagcaaaacg ttgtaacgcg gctgatagag tttgatgtag ttgtgttcca gcaacagcgc
     5821 ttcggtttct gtgtgagtaa ccgttacatc aatttgctgg atctgggcga ccagcgcttc
     5881 ggttttgcgc gaagcgaggt tgctacggaa atagctggaa agccgttttt tcaggtcttt
     5941 cgctttgccg acatagataa ccgtaccacc agcatcgtac atgcgataaa cgcctggctg
     6001 gctggttacg gtttttaaaa acgcttttgc gtcaaactga tcactcactg acttgataat
     6061 gtctccgcat tacacagacc atggcgaatt gccaggtgag tcagctcaac atcgccatga
     6121 atgtttagtt tactgaacat acgatagcgg tagctgttca ccgttttcgg actgagattg
     6181 agctgttctg agatctcatt gaccttctgg cccttggtga tcatcagcat aatctgcaat
     6241 tcacgttcag acaaactggc aaatgggctt tctgtttttt ctggttcgat ctggcttaac
     6301 gccatttgtt gagcgatgtc agaagcaatg taacgctgcc ctgaatagac agaacgaatc
     6361 gcactcacga cttcctgcgg agccgcgcct ttgctgaggt agcccgcagc accggcctgc
     6421 atgactttcg ctggtaaagg gttttctgta tggacggtaa gcatgatgat tttgacatca
     6481 gctgtggaac gcgcgatttt acgcgtcgcc tcaagaccgc caatgcccgg catactcatg
     6541 tccattagca ccacgtcaac ggcatttgtc cggcaccact taacggcgtc ttcaccgcac
     6601 gatgcctcac cgacgacttt tatacccttt atatcttcca gaatgcgtcg tatccctgcg
     6661 cgcaccagtt cgtggtcatc aacaagtaga acgttgatca aaggaatatc tccagaaata
     6721 gggataacgc tactgatagt tagtcattcg tatattagcg gtttttattg caactttgaa
     6781 acgttaaaaa tggtgggtat tcgatttttc tctcgttttt gtcaattcga ttgtccacag
     6841 ttatggtcac gcccgtcacc aacggcttac cggatcgttt cacagtaagc cctacgatga
     6901 aatgtgacaa aaattgactt tattcagcaa aaatgaaaat cagcccgctg
//
LOCUS       DAZGVQ010000225         6907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000225 DAZGVQ010000000
VERSION     DAZGVQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.227"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(185..670)
                     /gene="sixA"
                     /locus_tag="ACHLAG_004809"
     CDS             complement(185..670)
                     /gene="sixA"
                     /locus_tag="ACHLAG_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFP0657898.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(876..3017)
                     /gene="fadJ"
                     /locus_tag="ACHLAG_004810"
     CDS             complement(876..3017)
                     /gene="fadJ"
                     /locus_tag="ACHLAG_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFP0657899.1"
                     /translation="MEMASVFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTEVKKDPGSDAPPALLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISVTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDL"
     gene            complement(3017..4327)
                     /gene="fadI"
                     /locus_tag="ACHLAG_004811"
     CDS             complement(3017..4327)
                     /gene="fadI"
                     /locus_tag="ACHLAG_004811"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFP0657900.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4507..4791)
                     /locus_tag="ACHLAG_004812"
     CDS             complement(4507..4791)
                     /locus_tag="ACHLAG_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFP0657901.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5163..6503
                     /gene="fadL"
                     /locus_tag="ACHLAG_004813"
     CDS             5163..6503
                     /gene="fadL"
                     /locus_tag="ACHLAG_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFP0657902.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1740 a         1853 c         1626 g         1688 t
ORIGIN      
        1 tttcaacatg taactttggg caaaatcaag atacgcgcag aagaataatt tccgaatgaa
       61 tgtagaaagt gttttattta tagataagga gagtcttcga tagtagtgag gttgacagat
      121 atccgctgcc tgatgagcaa ttatgcccaa tgaatctacc tcattgggca taatttggaa
      181 cacatcagat agcttttgcc atcttcagat tacacgggct catctgccag ttgaatgtac
      241 cgtttccgct ttcatcaagc gttacgctgg caatggccga ggtggtaaac ataggcggcg
      301 tttcgcccgg acataactcg gcaaccagat acccgactaa cggtaagtga gagatcacca
      361 gtaccgaagc aacaccttca ttggtcaacg cctgcaaata ggcactgacc agaccaacat
      421 cgccgcaggg cgttaactcc ggcagaactt ctgcactgga aggcaggttc aaacaatccc
      481 ctacttcttc cagtgtttgc tcggctcgca ggaacgggct caccagaacg cgttcgattt
      541 ccactttttg acctttcagc cagttcgcca tcaggcgaga ttcgtcacaa ccattagtgg
      601 tcagaggacg aacggaatca ctggcggcat cgagggctgc gtcgccgtga cgcatgataa
      661 aaacttgcat attgcaccgc ttttgttaac cagattcgcc cgttttcttt catctgaaac
      721 cagagaaaga aaacggtggc cggcattgtg ccttatccat tcaccgaatg aaacgctgtt
      781 ttttacctca atggcgtaag tatagtcaat atgcgtttac attttgacca acactccgcc
      841 attcagcgcg gattcatata gctttgacct tcttattaca ggtcagttgc agttgttttc
      901 caaaaacttt ccccacgcgc gcccatctcg accaaacgct cgcaaggggt aaaacgggaa
      961 ccatactgcg tggcaagtcg ttgcattatt gcaaccactt cgcccgcgcc gagagaatcg
     1021 atatagcgga acggtccacc gagaaatggc ggaaaaccaa tgccaaatac cgcgccaata
     1081 tccccgtcac gcacgctacg gataacctgc tcatcaacac aacgtactgc ttcattcagc
     1141 atcaacatca cacaccgttc agcaacctgc ggtgcggaga gtcgcccctg cccttgtgcg
     1201 ccaatgagcg ggtaaatggc aggatcgacc tgttttttgc ttttacgccc tttctgacca
     1261 taaagataga aaccccgacc attttttctg cctttgcgat cgtcgttcaa aattgaagaa
     1321 acaacatttg caggcgcgct aaaacgttct ccataagcgg cttccagtac aggaataatt
     1381 ttagtcccgg tgtcgattcc tacctcatcc aaaagttgga ttgggcctac cggaaaacca
     1441 aatttcacta gcgcagcatc aatgtgctct acccgttcac cttcggtcag catgcggata
     1501 gcttcattaa tgtaaggcgc taagatgcga ttgacgtaaa acccggcttt gtcacgcacg
     1561 acaatgggcg ttttgccctg ttttttcgcc agttttaccg tagtggcgat ggtttgcgct
     1621 gatgtacccg catgaggaat aatctccacc agcggcattt tttccaccgg actgaagaaa
     1681 tgcaggccga taacttgctc aggtcgcgtg gcgtgagcgg cgatatcacc aatcggcaaa
     1741 gatgacgtat tcgaagcaaa gatggtatga gcggcgcaat tttgctccac ttccgccacc
     1801 atctgttgtt ttaattcgag attttcaaac accgcttcaa taatcagatc gcgatgggca
     1861 aagccgcgat agtccgtcgt tacggagatt aatgccagct gtttgtcacg ttcgctggct
     1921 ttgagatgac gacggcgaac tttgccctcc agctgatccc aactgtactt cagcgcatga
     1981 tttatgccct gcgggttgat atctttaatt ctgaccggaa gccccgcttt acaagcagtg
     2041 acataagcaa taccgccgcc catcaagcca ccacctaaaa ttcccacgct gtttaatagc
     2101 gcaggcggtg catcactacc gggatctttc ttcacttccg tactggcaaa aaagatacta
     2161 cgcaaagctt gcgattgtgg cgtcatcgcc agttcgccaa acgcccgagc ttcggcgtca
     2221 taaccgctgc tggtgccctg cgctaatccc gtttcaacaa cctccaggat gcgttctgtc
     2281 gccggataat tgccttgagt tttgtgttct gttttcttgc cgaccatttt gaacagcagc
     2341 gcacgaccta acggccccgc cagaatacgc tcgcgtacag gtagagggcg ggaagatggg
     2401 cgatccttct tcgccagctc aacagcgact tccagcaaaa tggagtgcgg aacaacgtca
     2461 tccaccagcc ccagctttaa tgcctgtttc gcccgaagtt gttttccggt gaggatcatc
     2521 tctaatgctg tgctgacgcc tatcagacgc ggtaaacgct gggtgccgcc tgaaccgggt
     2581 aacaatccaa gttgtacttc aggcaaaccg agcaccgttt taggatcgtc agtacaaacg
     2641 cgaccgtggc acgccagcgc caactccagc ccaccaccca ggcaagcgcc atgaatagcc
     2701 gcgataaccg gaatgggcaa agcatgaatc tccgccatca actgttggcc ctgccgcgcc
     2761 agtgcttccg cttcttgcgc cgttttgcag ttgccgatca tgttgatgtc tgcgccagca
     2821 atgaagttgt ccggtttagc ggagacaaac accacgcctc gcaactcttt gttttcacgg
     2881 agttgcttaa taatggcgcg cacctgcgag gcaaactccg ccttcagggt attcattttc
     2941 tcacccggta cgtcgatggt gatgatagca atgttgtcca gacgaacatt aagagtaaac
     3001 actgatgcca tttccattat tccgcctcca gaaccattgc cgcgccaagc ccaccggcag
     3061 cacaggcggt aactaaacca aatccaccgc cgcgacggcg aagttcatgc aatgtctggg
     3121 taatcatccg cgcgccggtc gccgcgaagg gatgcccgta agcaatcgaa ccgccaagca
     3181 cgttaaattt gctatcgtcc acttcgccag tggcatgtgc acgccccagt acttcacgag
     3241 caaaacgttc actacccagc aactgaatat tcgccagtgt ctgagctgca aaggcttcgt
     3301 gcatatcgat caatgtcaga tcgctcattg tcaaaccggc acgttccagc gccagcggtg
     3361 ttgaccaggc cggaccgagc aacatgtcct gccagacatc aatcgcagta aatgcgtagc
     3421 tgcgcagata ccccagcggc accagcccta attctttcgc ccgggattca gtcatcagga
     3481 tcaccgccgc tgcgccatcg gtcagcggcg tactgttcgc cgccgttacc gttccgtgtt
     3541 tgcgatcaaa cgccgggcgc agctttgcgt aatcggcaag cgaggaatta ccgcgaatat
     3601 tgttgtcttc gacaagcggt tgtttataag gagggataaa ggcagtcatc acctcttctt
     3661 tgagtttacc gtctgaccac gcctgagcgg cacgctgatg cgaacggtgc gctaatgcat
     3721 cttgctgttc tcgggtgatg ccgtaggttt tcgccatttg ttcggcggtg tcgcccatcc
     3781 gcaagccagt agaatattct gctaccgcag gcggtacggg cattaagtcg cgcaaacgca
     3841 ggcgagagaa gagtttcagt cgctggctca tggttcgagc tttgttgaca tcaaccagca
     3901 cgcgcgccag ttttttactg acgccaattg gcaatacgga agaggaatct gccccaccgg
     3961 caatccccgc tcggatagtt cccgccatca ggctttctgc gacgtttgca accgcctgga
     4021 aactggtagc gcaagcgcgg ctgacgctgt aagcatcggt atgcacattc attccggtac
     4081 cgagaacaat ttcacgcgca atgttggggg cttcaggcat ttgtacgacc tgaccaaaga
     4141 ccagctgttc aatcacttca gcggggatct cgctgcgtgc cagcagttcg cctaccacca
     4201 tcttccctaa atcaaccgcg ggaatgccat gaaaagccgt cgcctggcgg gcaaaaggcg
     4261 tacgtaaacc gctaacaatg gcgatacgat cgccctggcg ggtaaccagc ggtaaaacct
     4321 gacccataac actcccctgt aaaaaaataa ataaagtggt ctgacctgat catagtctta
     4381 accatttttt tacatttagc caagcggaga aaaaggaaag tgggagctat gacacagtga
     4441 agacaggaga aagaaaagaa aacgcccctg ccagcaactg acaggggcga acgtacccga
     4501 gaggaattaa cgcagaccca gctggaagat cagcatttca gcttcgcagg caaaagtgaa
     4561 atcgatatcc aggcgcacac cgtcagactc ttcagtgaaa gtcggggaga ttttgcacgg
     4621 ttcggattcc acgctacggg ctttttcagt cagcgccgcc agcgtttgtt ctgcttctgc
     4681 gcggtttgca aacacgcggc tgtaagacgc ggtgcagtcg gagttgtcca taatggtgcc
     4741 aacatccata cagcagcaaa ccggggtttc atcagcacta catttactca tcgttgattt
     4801 cctctgtatg tgcacccaag gtgccagata aacgttgtgg atattttacg cttccggaaa
     4861 gtgctgctcc agttgttaat tctgcaaaat cggataagtg accgaaatca cacttaaaaa
     4921 tgatctaaaa caaaattcac ccgaatccgc gagtgcgcaa cctccaaatt ttgccagctg
     4981 gatcgcgttt cttagatcat atttgaaaaa agatggaaac atacttgcaa cattccagct
     5041 ggtccgacct atactctcgc cactggtctg atttctaaga tgtacctcag accctacact
     5101 tcgcgctcct gttacagcac gtaacatagt ttgtataaaa ataaatcatt gaggttatgg
     5161 tcatgagcca gaaaaccctg tttacaaagt ctgctctcgc agtcgcagtg gcacttatct
     5221 ccacccaggc ctggtcggca ggctttcagt taaacgaatt ttcttcctct ggcctgggcc
     5281 gggcttattc aggggaaggc gcaattgccg atgatgcagg taacgttagc cgtaaccccg
     5341 cattgattac catgtttgac cgcccgacat tttctgcggg tgcggtttat attgacccgg
     5401 atgtgaatat cagcggaacg tctccgtctg gtcgtagcct gaaagccgat aacatcgcgc
     5461 ctacggcatg ggttccgaac atgcactttg ttgcaccgat taacgaccaa tttggttggg
     5521 gcgcttctat tacctctaac tatggtctgg caacagagtt taatgatact tatgcaggcg
     5581 gctctgtcgg gggtacaact gaccttgaaa ccatgaacct gaacttaagc ggtgcgtatc
     5641 gcttaaataa tgcatggagc tttggtcttg gtttcaacgc cgtctacgct cgcgcgaaaa
     5701 ttgaacgttt cgcaggcgat ctggggcagt tggttgctgg tcagattatg caatctcctg
     5761 ccgggcaaac tcctcaaggg caagcattgg cagctaccgc caacggtatc gacagtaata
     5821 ccaaaatcgc tcatctgaac ggtaaccagt ggggctttgg ctggaacgcc ggaatcctgt
     5881 atgaactgga taaaaataac cgctatgcac tgacctaccg ttctgaagtg aaaattgact
     5941 tcaaaggtaa ctacagcagc gatcttaatc gtgcgtttaa taactacggt ttgccaattc
     6001 ctaccgcgac aggtggcgca acgcaatcgg gttatctgac gctgaacctg cctgaaatgt
     6061 gggaagtgtc aggttataac cgtgttgatc cacagtgggc gattcactat agcctggctt
     6121 acaccagctg gagtcagttc cagcagctga aagcgacctc aaccagtggc gacacgctgt
     6181 tccagaaaca tgaaggcttt aaagatgctt accgcatcgc gttgggtacc acttattact
     6241 acgatgataa ctggaccttc cgtaccggta tcgcctttga tgacagccca gttccggctc
     6301 agaatcgttc tatctccatt ccggaccagg accgtttctg gctgagtgca ggtacgactt
     6361 acgcatttaa taaagatgct tcagtcgacg ttggtgtttc ttatatgcac ggtcagagcg
     6421 tgaaaattaa cgaaggccca taccagttcg agtctgaagg taaagcctgg ctgttcggta
     6481 ctaactttaa ctacgcgttc tgataacacg ttcgcctgga taaagtcacc tgcatagcag
     6541 gtgactttaa ctcccccact tcaccgaaag tagtgtcctc attgcttacc tcctgagttt
     6601 tgcaaacagc ctgatggcag cttgcccttt caaatcaata agcggtatcc acagacaaat
     6661 ctgaagggaa aggcatattt tcaggcgttc tgctcgtcct tctcaaagag tttacttttc
     6721 tgaatttcca ggatactccc cccccccctg gctattgtgc gctcatacac tcaaattaaa
     6781 gataggttct aaataaatga gcgttttttg atagtctatt tcattaggta atatatttgt
     6841 aacaaatcaa tcaaaatgga ataaaatcat gctaccatct atttcaatca acaataccag
     6901 cgcaact
//
LOCUS       DAZGVQ010000226         6860 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000226 DAZGVQ010000000
VERSION     DAZGVQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.228"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..359
                     /locus_tag="ACHLAG_004814"
     CDS             <1..359
                     /locus_tag="ACHLAG_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFP0657903.1"
                     /translation="DAAVGSVDRRVTKNTQAIQSNTRQLQEHNARLNSQQRQIRENHE
                     EMKRAAAQSAALAGLFQPYSVGKFNATAALGGYSDKQAIAVGVGYRFNEQTAAKAGIA
                     ASDGDVSYNMGVNFEF"
     gene            446..919
                     /locus_tag="ACHLAG_004815"
     CDS             446..919
                     /locus_tag="ACHLAG_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657904.1"
                     /translation="MKVILATRNRYLEYGLQQMLEGYRIILAREFFTPENRKSVPAHD
                     ESWVIICDALLGRLMCCMFQGRRYLQIDAEDVTGRLETYRKIRNGEWVHNTYARPLTM
                     SEMVVMFGYVYRESKPCHLAREMGINTKTVNTFLYMGLGKNGLKYRSVKHLVGRA"
     gene            complement(1131..1379)
                     /locus_tag="ACHLAG_004816"
     CDS             complement(1131..1379)
                     /locus_tag="ACHLAG_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP0657905.1"
                     /translation="MRIEICIAKEKICKMPKGSVPALQQEMLRRVGKRYDDVDVIVKS
                     ASNDGLTILRAVDKESAQEFVQETLQEVWETADDWFIR"
     gene            complement(1614..1709)
                     /locus_tag="ACHLAG_004817"
                     /pseudo
     CDS             complement(1614..1709)
                     /locus_tag="ACHLAG_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1706..2272)
                     /gene="yecD"
                     /locus_tag="ACHLAG_004818"
     CDS             complement(1706..2272)
                     /gene="yecD"
                     /locus_tag="ACHLAG_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP0657906.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            2582..4354
                     /gene="aspS"
                     /locus_tag="ACHLAG_004819"
     CDS             2582..4354
                     /gene="aspS"
                     /locus_tag="ACHLAG_004819"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFP0657907.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4472..4924
                     /gene="nudB"
                     /locus_tag="ACHLAG_004820"
     CDS             4472..4924
                     /gene="nudB"
                     /locus_tag="ACHLAG_004820"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFP0657908.1"
                     /translation="MEDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            4953..5693
                     /gene="yebC"
                     /locus_tag="ACHLAG_004821"
     CDS             4953..5693
                     /gene="yebC"
                     /locus_tag="ACHLAG_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0657909.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            5728..6249
                     /gene="ruvC"
                     /locus_tag="ACHLAG_004822"
     CDS             5728..6249
                     /gene="ruvC"
                     /locus_tag="ACHLAG_004822"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFP0657910.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(6251..6853)
                     /gene="yebB"
                     /locus_tag="ACHLAG_004823"
     CDS             complement(6251..6853)
                     /gene="yebB"
                     /locus_tag="ACHLAG_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFP0657911.1"
                     /translation="MNINYPAEYEIGYIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
BASE COUNT         1719 a         1639 c         1888 g         1614 t
ORIGIN      
        1 ttgatgctgc agttggtagc gttgaccgcc gggttactaa aaatacacag gctattcagt
       61 ccaatactcg ccagttgcag gagcataatg cacgcctgaa cagccagcag cgccagattc
      121 gtgaaaacca cgaagagatg aagcgtgcag cagcacagag tgcagcgctg gcgggtcttt
      181 tccagccgta cagtgtgggg aaattcaacg ccacggcagc cctgggtggt tacagcgata
      241 aacaggcgat tgctgtaggt gttggttacc gtttcaacga gcagaccgca gcgaaagcgg
      301 gtattgcagc gagtgatggc gatgtgtcct acaacatggg cgttaacttc gagttttaat
      361 tgctgacagt gacatgaagc atgacggcag gcgtggagtt ttgcctgccg ttttttctca
      421 tgtgagcatg aaacaggtga cagcgatgaa agtgattctg gcgacccgca accgttatct
      481 ggagtatggc ctgcagcaga tgcttgaggg gtacaggata atactggcga gggagttttt
      541 tacaccagag aacaggaaga gtgtaccggc acatgatgag tcatgggtga taatctgtga
      601 tgccctcctt ggcaggctga tgtgctgcat gtttcaggga cgccgttatc ttcagattga
      661 tgcggaagat gttacggggc gcctggagac gtacaggaaa atccggaatg gtgagtgggt
      721 acataatacg tatgcgcgac ctctgacgat gtccgagatg gtggtgatgt ttggatacgt
      781 gtaccgggag tcaaagccct gccatcttgc ccgtgaaatg gggataaaca cgaagacggt
      841 gaacaccttt ctgtatatgg gacttggtaa aaacggtctt aagtacagga gtgtgaagca
      901 cctggttgga cgtgcgtagc cggaatgaat attcgtgagt cgggtaatga gagttgaacg
      961 gtggcagtga gaatggagca ggaaaaaatc agatgcggaa agcggaaata cacagcgggt
     1021 gcgatgtgct tcatgctgtt tgtatggctg ggtggtgtgg tgacactctg tgtacttgct
     1081 gcactgataa cacaataaaa gaggggagct gccagctccc cgtgctgatg tcaccggata
     1141 aaccagtcat ccgctgtttc ccagacttcc tgcagcgttt cctgtacaaa ttcttgcgcg
     1201 gattctttat ccacggctct taaaattgtg agtccatcat tactggcaga tttcacaatc
     1261 acatctacat catcataacg cttaccgaca cgtcgtagca tttcctgttg caaggcagga
     1321 acagagcctt ttggcatttt gcagattttt tctttagcga tacagatctc aatacgcata
     1381 acaccatcct gtcactgtgc ttatgtacag tcgttatttt tatctgtatg gataaacagt
     1441 gtcaatcatt cggattggtt atgggcatat atgggacgct ttggggcatg tatgggacat
     1501 ttttaatcgg cgaaatccgc cgaagttcgt cgacatggta aacgaatcat ctatccaacc
     1561 cttgaaaaac ggcgctcctg gacgatcttc gtcgattttt aaaaatgttg cgtcacgcag
     1621 ttaaagtggc gggcatactc ttcaaggctg gtgatcccca accgcaccca tttaggatgc
     1681 gaccattgcg gtagaccgat gtaaatcata acgcgtgaag gatctcttcc acactgcgca
     1741 cacgggcgat gcgtgggtag atatggttga tgctgttatt gtgctgctcg gcgctggcgg
     1801 cactacaggc gtcttcggca atcaccagat taaagccaag ttcccaggca ttgcgggcgg
     1861 tggattcaac accgatattg gtcgagatcc cacataacac tattgtatcg atacctcggc
     1921 ggcgcagttg caactccaga tccgtaccgt aaaacgcacc ccactgacgt ttgatgattt
     1981 cgatatcact gtcagttgca cctaatgcag ctggatgttg ccaccagttt tcgggcaaca
     2041 ctttagcggg ggagggggca tcaaccggct gttttaatgc ttcggcgtaa tcggcagacc
     2101 agccaacgcg caccagaaac acgggctgac cgctggcgcg aaatttcgcc gccagcttcc
     2161 cggcgcgatt aactacctca tcggcagtat gtggaccacc ggcaaaaggt aagatgcctt
     2221 cttgtaaatc gatcaccacc agcgcagtgg ttttagcatt aagttcaagc ataatgactc
     2281 ctgaaaaaac aaaatcgtgc ctcacacctt aatgcgatta gccgatggcg gaacgacaaa
     2341 ttttgttaat ttttatgagt tcgggcaaca cctttatagc aaactggcgt agagtctgat
     2401 ttcaggcccg atataaagtg gtaacgaggc gttccagcgc gggccaggct cttacggtgc
     2461 ggcagaattt ccagtataat agccgccttt tttcattcag ttgtgacata cagctaacgc
     2521 tgcgacttgg tcacctgcgc agcagggggc atccgccggc agctaagtta agggatatct
     2581 catgcgtaca gaatattgtg gacagctccg tttgtcccac gtggggcagc aggtgactct
     2641 gtgtggttgg gtcaaccgtc gtcgtgatct tggtagcctg atcttcatcg atatgcgcga
     2701 ccgcgaaggt atcgtgcagg tatttttcga tccggatcgt gcggacgcgt taaagctggc
     2761 ctctgaactg cgtaatgagt tctgcattca ggtcacgggc accgtacgtg cgcgtgacga
     2821 aaaaaatatt aaccgcgata tggcgaccgg cgaaatcgaa gtgctggcgt cctcgctgac
     2881 tatcatcaac cgcgcagatg ttctgccgct tgactctaac cacgtcaaca ccgaagaagc
     2941 gcgtctgaaa taccgctacc tcgacctgcg tcgcccggaa atggctcagc gcctgaaaac
     3001 ccgcgctaaa atcaccagcc tggtgcgccg ttttatggat gaccacggct tcctcgacat
     3061 cgaaactccg atgctgacca aagccacgcc ggaaggcgcg cgtgactacc tggtgccttc
     3121 tcgtgtgcac aaaggtaaat tctacgcgct gccacaatcc ccgcagttgt tcaaacagct
     3181 gctgatgatg tccggctttg accgctacta tcagatcgtt aaatgcttcc gtgacgaaga
     3241 cctgcgcgct gatcgtcagc ctgaatttac tcagatcgat gtggaaactt ctttcatgac
     3301 cgcgccgcaa gtgcgtgaag tgatggaagc gctggtgcgc catctgtggc tggaagtgaa
     3361 gggcgtggat ctgggcgatt tcccggtaat gacctttgcg gaagcagaac gccgttatgg
     3421 ttctgataaa ccggatctgc gtaacccgat ggaactgacc gacgttgctg atctgctgaa
     3481 atctgttgag tttgcagtat ttgcaggtcc ggcgaacgat ccgaaaggtc gcgttgccgc
     3541 tctgcgcgtt ccgggcggcg catcgctgac ccgtaagcag atcgacgaat acggtaactt
     3601 cgttaaaatc tacggcgcga aaggtctggc ttacatcaaa gttaacgaac gcgcgaaagg
     3661 tctggaaggt atcaacagcc cggtagcgaa gttccttaat gcagaaatca tcgaagccat
     3721 cctggatcgt actgccgcgc aagatggcga tatgattttc ttcggtgccg acaacaagaa
     3781 aattgttgcc gacgcgatgg gcgcactgcg cctgaaagtg ggtaaagacc ttggtctgac
     3841 cgacgaaagc aaatgggcac cgctgtgggt tatcgacttc ccgatgtttg aagacgacgg
     3901 tgaaggcggc ctgacggcaa tgcaccatcc gttcacctca ccgaaagaca tgactgctgc
     3961 agaactgaaa gctgcaccgg aaaatgcggt ggcgaacgct tacgatatgg tcatcaatgg
     4021 ttacgaagtg ggcggtggtt cagtacgtat ccataatggt gatatgcagc agacggtgtt
     4081 tggtattctg ggtatcaacg aagaggaaca gcgcgagaaa ttcggcttcc tgctcgacgc
     4141 tctgaaatac ggtactccgc cgcacgcagg tctggcattc ggtcttgacc gtctgaccat
     4201 gctgctgacc ggcaccgaca atatccgtga cgttatcgcc ttcccgaaaa ccacggcggc
     4261 agcgtgtctg atgactgaag caccgagctt tgctaacccg actgcactgg ctgagctgag
     4321 cattcaggtt gtgaagaagg ctgagaataa ctgatatgac tcaaatacac gaaatcattc
     4381 gcgttgcatc aaggcggcaa ctgagtgaac tcccatgagc atagataact atgtgaatgg
     4441 gatgagcgaa ggcagtcaac gaagaggcag cgtggaggat aaagtgtata agcgtcccgt
     4501 ttcgatctta gtggtcatct atgcacaaga tacgaaacgg gtgctgatgt tgcagcggcg
     4561 tgacgatccc gatttctggc agtcggtaac cggcagcgtg gaagagggtg aaaccgcgcc
     4621 gcaagctgcc atgcgcgaag taaaggaaga ggtcaccatt gatgttgtcg ctgaacaact
     4681 gaccttaatt gactgtcagc gcacggtaga gtttgaaatt ttttcacatt tacgtcatcg
     4741 ctatgcgccg ggcgtgacgc gtaatacgga atcatggttc tgtcttgcgc ttccgcacga
     4801 gcggcagatc gttttcactg aacatctggc ttacaagtgg cttgatgcgc ctgctgcggc
     4861 ggcgctcact aagtcctgga gcaaccggca ggcgattgaa cagtttgtaa ttaacgctgc
     4921 ctgaacaggc agggctatct ggagatattt ttatggcagg tcatagtaaa tgggccaaca
     4981 ccagacatcg taaagctgcg caggatgcta agcgcggtaa aatcttcact aaaatcattc
     5041 gtgagctggt aaccgcggct aagctgggcg gtggcgatcc ggacgctaac ccgcgtctgc
     5101 gtgcggcggt ggataaagca ctgtctaaca acatgacccg tgacacactg aaccgcgcaa
     5161 ttgcacgtgg tgtgggcggt gatgatgatg caaacatgga aaccatcatc tacgaaggtt
     5221 acggtcctgg cggcacggca atcatgattg aatgtctgtc tgacaaccgc aaccgtaccg
     5281 ttgctgaagt gcgtcatgca ttcagcaaat gtggcggtaa cctcggtaca gatggttccg
     5341 tagcctatct gttcagcaaa aaaggcgtga tctccttcga gaaaggcgat gaagacacca
     5401 tcatggaagc agctctggaa gcaggtgctg aagacgttgt gacctatgat gacggcgcga
     5461 ttgatgtcta cactgcatgg gaagaaatgg gtaaagtgcg cgacgctctg gaagcggcag
     5521 gtctgaaagc agacagcgcg gaagtttcca tgatcccgtc caccaaagct gatatggatg
     5581 cagaaaccgc accgaaactg atgcgtctga tcgatatgct ggaagattgc gacgacgtgc
     5641 aggaagttta ccataacggt gaaatctctg atgaggtcgc agcgactctc tgatgaggcc
     5701 tgctaaacag caaaacggag acgcgtgatg gctattattc tcggcattga tccgggttcg
     5761 cgcgtgaccg gctatggcgt aattcgccag gtaggcaggc aactgtccta cctgggtagc
     5821 ggatgcatac gcaccaaagt ggatgattta ccgtctcgtc tgaagctcat ctatgcgggc
     5881 gtgacggaaa tcatcaccca gttccagcct gattatttcg ccattgaaca agtctttatg
     5941 gcgaagaacg ctgactcagc cctgaaactg ggccaggcgc gcggcgtggc gattgtggcg
     6001 gcggtgaatc aggagttgcc agtatttgaa tacgcggcac gtcaggtaaa gcaaacggtg
     6061 gtaggcattg gtagcgccga gaaaagccag gtgcagcata tggtccgcac cttgctgaaa
     6121 ctgcccgcta atccacaagc ggatgccgcc gatgcactgg cgattgccat cacccactgc
     6181 cacgttagtc agaatgcgat gcagatgagc gaatcgcggc tgaacctggc gagagggcga
     6241 ctgcgttaag ttataccgcc tcggtcagtt caggctgagg cgtttcaact ccctccgcgt
     6301 gaatcaacac caaacccgga tgatgccaca aactggctgg cgtgacggtt ttacgctccc
     6361 acggaataga acctaagaac cagaatttcc agaaagtgag ttttgcattc ggattgctat
     6421 ttaacagttc tccaaacgtt tctatttcac ccaccggaat gcatagcgct tctttataaa
     6481 tatcaaaaac aaattttgaa cagaactggc gcgaagattc gtatttaaaa ccggtgtggt
     6541 aaagtttgcg tagccgggag ggaacctgtt caacaattcg ttgtttttgt tgttctgtca
     6601 gcccagcgtc taatcgcttt atagcatagc gttgattaga cgaacgttta ataaaacgag
     6661 atagcgtggt gatggttgaa aggggaaccc ggctttctgc aaccaggaag tcttcaccgt
     6721 tatgaccgat aatgatcccg acgtgattac tccagcaatt tgatgcagct gatatttgac
     6781 caaataaggc agcacctata catgtaaaga cgatatatcc aatttcatat tcagcagggt
     6841 aattaatatt cacgttgtca
//
LOCUS       DAZGVQ010000227         6830 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000227 DAZGVQ010000000
VERSION     DAZGVQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.229"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..272)
                     /locus_tag="ACHLAG_004824"
     CDS             complement(<1..272)
                     /locus_tag="ACHLAG_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HFP0657912.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRV"
     gene            complement(269..1939)
                     /gene="menD"
                     /locus_tag="ACHLAG_004825"
     CDS             complement(269..1939)
                     /gene="menD"
                     /locus_tag="ACHLAG_004825"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFP0657913.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     STFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTRDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(2028..3323)
                     /gene="menF"
                     /locus_tag="ACHLAG_004826"
     CDS             complement(2028..3323)
                     /gene="menF"
                     /locus_tag="ACHLAG_004826"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFP0657914.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(3402..3707)
                     /gene="elaB"
                     /locus_tag="ACHLAG_004827"
     CDS             complement(3402..3707)
                     /gene="elaB"
                     /locus_tag="ACHLAG_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFP0657915.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(3762..4223)
                     /locus_tag="ACHLAG_004828"
     CDS             complement(3762..4223)
                     /locus_tag="ACHLAG_004828"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0657916.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            4288..5205
                     /gene="rbn"
                     /locus_tag="ACHLAG_004829"
     CDS             4288..5205
                     /gene="rbn"
                     /locus_tag="ACHLAG_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFP0657917.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGMLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            5393..6612
                     /gene="elaD"
                     /locus_tag="ACHLAG_004830"
                     /pseudo
     CDS             5393..6612
                     /gene="elaD"
                     /locus_tag="ACHLAG_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase ElaD"
BASE COUNT         1774 a         1778 c         1589 g         1689 t
ORIGIN      
        1 acccgtccgc caagcgagta ccccaccagc caaaagtcaa ggatgttgta actaaccaag
       61 gttttacgca gtaagtcggt gacatcatca aatccatcga cgctaatcgc cgccgaacca
      121 ccatgacctg ggagatcaac atacaaccgt gagtagtcgg caaacgcctc gcccacttct
      181 tgccattcgt ggcaatcgcc ggaaaaaccg tgcaaaaaca ccagccaggg taaacctggt
      241 tttccgtgtt ttgcctgcgc gtgcaggatc ataaatggct tacctgcgcc agaagttgct
      301 ggagcgtttg cgcaccatcg gtgtcgttaa ccaccatttc aatcaccgtg gtggttggcg
      361 tgcgccaggc gtcggcaaat gccgtttcaa gttcctgcca gttttgcgga cgatgatatt
      421 tcagctcgaa catcgcggcg gcgtgctcaa aatggacgtt ttgcggcatc agatagaaac
      481 gctcacgctc gctttgcggc gttggcaaca gcgagaaaat ttgcccgccg ttgttgttca
      541 ccacaattaa taccagcggc gcagaaacct gacgcaataa cgccagcgcg ttgagatcgt
      601 aaagtgcgga gagatcgccc acaatcgcca gcgtcggttt gccgcttgcc cgctgaacgc
      661 ctgcggcggt cgaaagcagt ccgtcgatac cgctggcccc tcggttgctg tacaccgggt
      721 aacctgccgg aagttgcgaa agcgcatcaa tcagacgtac caccaggctg ttaccaacaa
      781 acaattgccc ctgttcaggc agatagtcgc agatgcgatg cgccagttgc gcttcgccaa
      841 acgcatcacg gcgggcaata accgcctgca ttgcctgttc cgccaggcgc gggatttcaa
      901 cgcaccaggg ctggcgtttt tctgccggat gcagctccag ccagtcggca atattggcaa
      961 ttaagcgacg tccgcgatgg tgtgccggat caagtcgccc ttcaatgtca tcaacaatcc
     1021 agtactcttc tggttcacag cttgcctgcc attgcaggag ccgtttgccc gtcaggctgc
     1081 ttcccagttg caccacaatt tgcgcctgct gcagctcgct ggtcgctttg gcattgccta
     1141 accaaagatc ggcacacggc agcggctgcc cggtttgtga cagcacatcg ccaatcagcg
     1201 gccagccaag agtttgcgcc cacagggcaa cttttttgcc ctcttccgca ctcatgcgcc
     1261 cggcaaccac cacgccgcgc ttttgtcgcc agaagaacca gtcgcgctgt ttttcacttt
     1321 ccagacgagg cgcttcacgc agccacggtt tgtcgtcctg ccaccagtca cccagacgct
     1381 gttgccagct aagcccggta tcgtccattt cgccatacag cggttcagca aacgggcagt
     1441 tgatatggac tccccccgca tgaagcgtac cgagagcgtg gtcgatggta gaaaccagcc
     1501 aacgtgcggg gatatcccgg gtcgggcgcg gcaatgaaat actgtgcgtg gggtgagagg
     1561 cgaacattcc cggctggcga attgcctgat tcgcgccgca gtcaattagc tccggcgggc
     1621 gatcggcggt taacaggatc agtttttctc cggttaaccc agcttcaatc agtgccggat
     1681 agagatttgc taccgccgtg ccggaggtca caatcaccgc caccggctgc ttgctgactt
     1741 tcgccagccc cagcgccaga tgtcccagtc cacgctcatc gaaatgggtg tggtgaatga
     1801 atgtggaatt ctccgccgcc gctaacgtta acggtgtaga acgcgagcca ggggcgatac
     1861 agatgtgtct gacgccgtga cgcgttaatg cttccagaat gaccgccgcc cagcgtcggt
     1921 taaatgcgct tactgacatg agtttgtccg gtatcaatat tgcggctaag tataaggagc
     1981 tacaaaaata gattattgat atgaatcggt aatgatgcga ctcattacta ttccatctgt
     2041 aataaagtac gcagccctgc cgctttgttg tcgatttcct gccactcttg ctcggggtcg
     2101 gaaccacgga caatgcccgc gccagcatat aatcgcacga cattgccgct aatttttgct
     2161 gagcgcaggg aaacgcagaa ttcgctttgt tgtaatgaga gatagcccgc agaaccggcg
     2221 taccattctc gggtgaacgg ttcgtgacgg gcgataaact gtcgcgccag atcgcgcggt
     2281 aagccagcaa ctgctgccgt tggctgcaac tgatgtaaac agatcacatc atccgctttg
     2341 ttgagtgaag tccagataca gcggcgaaga tgctgcactt tacgcagacg cagtacctgc
     2401 ggcggtaaaa catccagcgt ctgggtatcg gcctgtaatc gttggcagat atcttccacc
     2461 accagcatgt tctcgcgctg gtttttatca tccgccatca gccactctcc taactgctgc
     2521 gcctgcttat catcaggatt atttgctact gttcccgcca gcgcttcagt acgcagcgct
     2581 ttgtcacgcc gccgccataa ccgttccggt gaagagccaa gaaaagcatt ttcgccatca
     2641 aaggccatgt aaaaatggta gcaattcaga ttcagtcgac gactggcagc catcatcgcc
     2701 gccgcgttga ccggacttgc gaaatgcagg tcagttgccc gagcgagcac cactttgtcg
     2761 agctcacctt cggcgatggt tttcgttgcc agttcgatta attgcgtcca gcccgttttg
     2821 tccggccagt gttgttctcg cgtggtggtt aaatgtaacc caggcaaggg cttgatactc
     2881 accagtgtgg cgataaattc ttttgcctga atcgcatcgt gctgaaggga gctttcgctg
     2941 aatagcgtca gccgcagcgt ggctttaccg ccacagcgtc gccattccag gcggggtaaa
     3001 agtaaattgc cctgcgacgg gtcaaaagca ttcagccccc aaatgcgtaa gtcggcgtgt
     3061 tccgggtgct ggcgaagaaa acgttgtgcc tggtccaacg acgtaaaacg ggtaatcgcg
     3121 cccaggacgg cagcttcttc atcaccatta cgttgttgcc agtagaattg cgggtacgtt
     3181 tgctgactgg ccagccagct caaggcatca aaagcgtctt tgagagggaa aggaatatcg
     3241 ataacccgaa tgccgggtgt cgccggaatc tcttgcgaca aatggcgcag tagattttcc
     3301 agcgccgtag taagtgattg cacgcggacc tctccctgtt aaaagcctca cattatacgg
     3361 ggtactacaa aaaaaatgca gtaccccggt gtagggaggt tttaacggcg tgccagcaac
     3421 agtcctagta ccagcccaac ggccgcgccc acaccaattc cttgccaggg tttttcgtgg
     3481 acgtagtcat cagcacgata aactgcctgc ttcgcccgat agtaataact gtctgaagcc
     3541 tggctaaccc gttttttcac atcatccagc gctttttctg cacgcgcttt cagctcaaca
     3601 tatttctgat cggcgggatc gccagaggag cggagcacct cttccagtgt ttcactaagc
     3661 agcgtcaggt cgtcatcgat acgtgtatca ccaaactgat tagacattct cgttctccat
     3721 ttgcgtaaaa cctgtcacta actatagaca agggttgttg attacgcctg aattacctcg
     3781 cgtgccatcc caatgtgtgg gataccatct tcttcataga cctccgtcac agggataaaa
     3841 ccgaaactct ggtagaagtt ttgcagatgc gcctgcgccc cgaggtaaac aggcttatca
     3901 ggccagtgat gcgtacatgt ttcgagtgtt ttactcatca gttgctgccc cactttttcg
     3961 ccacgcaacg cttcactgac aatcacccga cctataacga ccggctcaag atcatcatca
     4021 cttttcagaa tcctcgcata tgccaccagt tcatcatttt tccaaccaag aatatggcga
     4081 ttatcccccg tcaaatcatc accatcgata tcctgataag ggcaattctg ctcgacaaca
     4141 aacaccgcgc aacgcagttg taataaggca taaagctggg aaaccgataa ttcagagtga
     4201 tgcagatctt gccattcaat cataatgctc tccttttaga tgtatgcctt atactagccg
     4261 gaatattttt tgaaacgtga tgaactcatg gaattaattt ttttaggtac ttcggccggt
     4321 gtgccaaccc gcacgcgcaa tgtcacggca atattgctta acctgcaaca tccgacccag
     4381 agcggacttt ggttgtttga ctgcggtgaa ggcacccagc atcagctact gcataccgcc
     4441 tttaaccctg gaaaactgga caagattttt atcagtcacc ttcatggcga tcatcttttt
     4501 ggtttacccg gcttgctgtg cagtcgttct atgtcaggca ttatccaacc cttaacgatt
     4561 tatggtccgc aaggtatccg tgaatttgtg gaaaccgcgc tgcggattag cggctcatgg
     4621 accgattatc cgctggaaat tgtcgaaatt ggcgctggcg aaattctcga tgatggcctg
     4681 cgcaaagtaa ccgcttatcc gctggaacac ccactggaat gttatggcta tcgtattgaa
     4741 gaacatgata aaccgggtgc attaaatgcc caggcattaa aagctgctgg cgtgccgcct
     4801 ggtccactgt ttcaggaatt aaaagcgggc aaaacaatca ccctggaaga tggaaggcag
     4861 attaacggcg cagattactt agctgctcca gtgccaggta aagcgctcgc tattttcggc
     4921 gataccggcc cctgcgatgc cgcacttgat ctggctaaag gtgtcgatgt catggtgcac
     4981 gaagcgacgc tggatataac catggaagcc aaagccaata gtcgcggcca tagctctaca
     5041 cgccaggctg cgacactggc ccgtgaggct ggagtcggta tgctaatcat tacccacgtc
     5101 agctcgcgct atgatgacaa aggttgtcag cacctgttac gtgaatgcag gtcaattttc
     5161 ccggcgactg aactggcgaa tgatttcacc gtgtttaacg tttaactctg cttattatta
     5221 aaaacagggc gaaacttgcc ctgttatcgc aacccgcgct acatatatcc acccacacgg
     5281 gcatattgag cgatttttga tatttattca tctcctcacg cactatattc agagcaataa
     5341 gttgcctcta tttatcttga ttcagcacaa cctttgcaac taaaaggagc ccatgatggt
     5401 tacagttgtc agcaattatt gtcaattatc tcaaaagcaa ctcagtcaga catttgcaga
     5461 aaaatttact gtgaccgagg aattactgca gtctttaaaa aaaacagcgt tatccggaga
     5521 tgaagaaagc attgagttac tgcataatat tgcgttaggt tatgataaat ttggaaaaga
     5581 agctgaagac attctttacc atattgttag aaacccaaca aatgagaccc tatcgattat
     5641 ccgacttatc aaaaatgctt gtttaaaatt atataatttg gcacataccg caaccaactc
     5701 cccccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
     5761 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
     5821 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg
     5881 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
     5941 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
     6001 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
     6061 agtccagtta ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat
     6121 agtaaataaa aaaataataa actacaaata aaatgcgtca tattcaactc attgcgtgca
     6181 ctaggccatg ataaagaaaa ttcacttacg cgtgtcatta atagtttcaa ttctgaactc
     6241 atgggagaaa tgtcgaataa taatataaaa gttcatttaa atgaaccaga gataatattt
     6301 ttacatgccg atcttcagca atacttaagc caaagttgcg gtgcgtttgt gtgcatggca
     6361 gcccaggaag tgattgaaca aagggaaagc aattctgaca gcgcccccta tacgttatta
     6421 aaaaactatg ctgacagatt taaaaaatat tcagcagaag agcagtacga aattgatttt
     6481 caacatcgac tgataaacag aaattgttat ttagataaat atggcgatgc taatatcaat
     6541 cattattata aaaacttaga aataaaacac acacaaccca aaaatagagc gtcaggaaaa
     6601 agagtaagtt aacatcattg ttaattaatt cgaccagtca gtaaatatgc tgactggtag
     6661 gacatagttt gctaaaaagt catcactgac tgatgccagt cacaccatcc gccagttcaa
     6721 tcttcgggta tttccagcgt atctcggcac gcgattgctg gtctgttgat gtagtggatt
     6781 gttgttgctg acttttctta ccatcagatt tctgccccct gtcaactcaa
//
LOCUS       DAZGVQ010000228         6815 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000228 DAZGVQ010000000
VERSION     DAZGVQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.230"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(128..796)
                     /gene="artM"
                     /locus_tag="ACHLAG_004831"
     CDS             complement(128..796)
                     /gene="artM"
                     /locus_tag="ACHLAG_004831"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFP0657918.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(796..1512)
                     /gene="artQ"
                     /locus_tag="ACHLAG_004832"
     CDS             complement(796..1512)
                     /gene="artQ"
                     /locus_tag="ACHLAG_004832"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFP0657919.1"
                     /translation="MNEFFSLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1519..2250)
                     /gene="artI"
                     /locus_tag="ACHLAG_004833"
     CDS             complement(1519..2250)
                     /gene="artI"
                     /locus_tag="ACHLAG_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFP0657920.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDSTCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2268..2996)
                     /gene="artP"
                     /locus_tag="ACHLAG_004834"
     CDS             complement(2268..2996)
                     /gene="artP"
                     /locus_tag="ACHLAG_004834"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFP0657921.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3214..3729)
                     /gene="ybjP"
                     /locus_tag="ACHLAG_004835"
     CDS             complement(3214..3729)
                     /gene="ybjP"
                     /locus_tag="ACHLAG_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP0657922.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3855..4178
                     /gene="ybjQ"
                     /locus_tag="ACHLAG_004836"
     CDS             3855..4178
                     /gene="ybjQ"
                     /locus_tag="ACHLAG_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFP0657923.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4175..5005
                     /gene="amiD"
                     /locus_tag="ACHLAG_004837"
     CDS             4175..5005
                     /gene="amiD"
                     /locus_tag="ACHLAG_004837"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFP0657924.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(5002..6015)
                     /gene="ybjS"
                     /locus_tag="ACHLAG_004838"
     CDS             complement(5002..6015)
                     /gene="ybjS"
                     /locus_tag="ACHLAG_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP0657925.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6114..>6815)
                     /locus_tag="ACHLAG_004839"
     CDS             complement(6114..>6815)
                     /locus_tag="ACHLAG_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFP0657926.1"
                     /translation="VWFLNVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAF
                     DDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKTSASLAALWQVV
                     NQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEK
                     QLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFR
                     GMAKQIARLAEQSTD"
BASE COUNT         1651 a         1792 c         1799 g         1573 t
ORIGIN      
        1 tatgcactat gtaggccgga taaggcgtcc acgccgcatc cggcacaggc accgtgctga
       61 tgtctgatgc gacgctggcg cgtcttatca gacctacaaa cccccccggc gaatgtacgc
      121 agctacatta atttcgccgt tcgaatgcca gtgctttgcg ctcgatcaga cgcatcatca
      181 gcgtcagcag gccgttgacg accaggtaaa taatccctgc cgcaccgaac accattacat
      241 cgtaggtgcg tccgtacaac aactggctgt atcccatcac ttccatcagc gtaatggtgt
      301 aagccagaga ggtacttttg aacaccagca ccacttcgtt ggaataagaa gagagcgagc
      361 gtttaaaggc atacggcagc aggatcgcca gcgtatcttt tttgctcatt cccagggcgc
      421 tacaggactg ccactgacct tctgggatcg cacgaattgc accgtaaaac agctgcgtgg
      481 tatacgccgc actgttcagc gataacgcaa tcagcgcaca taaccacggt tctgacaaca
      541 aatgccacag tgccggatac tcctgcaaag tcggaaactg gcccggcccg taataaatca
      601 ggaagatctg caccagcagc ggcgtaccgg taaacagcgt gatataaccc cgcaccagcc
      661 acaccagcac cggcgttttc agcgtcagga tgatggtaaa aatcaatgcc agaatcagtg
      721 ccacaatcag cgaggcaacg gttagcgtca ggctggtgtg tagccctttc atcagttcgg
      781 gtaaatactc aaacattagc tgggcctccg ctcaaaacgt gtcgcgcgca ggtcaatgcg
      841 tttgagaatg tactgactga gcagggtgat caccaggtaa atcgccgccg ccacaatgta
      901 ccaggtaaat ggttcctggg tacgagtagc gatgcttttt gtttgcagca ttaaatcatt
      961 cacactaatc aaactgacca gcgcggtatc tttcagcagc accagccact ggttaccgag
     1021 gccaggcagc gcatgacgcc acatctgcgg catcaccaga cggaaaaaga tagccgattt
     1081 cgacagcccc agcgcctgac cagactccca ctgacccacc ggcaccgctt tcaacgcgcc
     1141 ccgaagcgtt tgcgaggcat aggcggcata cagcagtgac agagcgatga caccacaaag
     1201 gaacgggcta acgtcgaagt tctcaatgtc catctgcact gggatttgca cgaacccaag
     1261 attgatagtg aagccatccg aaagcgtcag cagcagctgc gaggagccaa aatagataaa
     1321 cagcaccacc agaatttctg gcaggccacg cagaatggtt accagcgctg aacctgccca
     1381 cgcgacagga cgccattttg ccgactccca taccgcaaag aacatcgcca gcgccagccc
     1441 gacaatcaat gcacaaacgg caaggccgac ggtcatcccg gcggcgcttg ctaaagaaaa
     1501 aaattcattc atcaggaatt acttctggaa ccatttgttg tagatggttt cgtaagtgcc
     1561 atctttcttc actttttcca gcgcagtgtt gagtttctgc tgcagctcag tgttgccctg
     1621 acgtaccgcg atgccgaggc cagtgccgaa gtaatcttta tcggtcactt tgtcgcccac
     1681 cgccgccagt ttcgggttat ctttcagcca ctcagtgacc actgcggtgt caccgaagac
     1741 gccgtcgata cgcccgtttt gcagatccag ttttgcgttc tggtagctgt catacggaac
     1801 ggtagtgatt tccgggtgct tatccataat gaatttctgg tgtgtcgtcc cgttctgaac
     1861 gccgactttt ttgcctttca gctgatcaac actggtgtat ttgccttgct gacccacaaa
     1921 cagggcagag ttgtcatagt acggggtggt aaacagtacc tgcttttcac gctccggagt
     1981 gatatccatg cccgccatca ctgcttctac gcgacggaat ttcaggcttg ggatcaggct
     2041 gtcaaacgcc tggttagaga aagtacaggt tgaatcaatc tctttacaca gtgcctgtgc
     2101 caggtcgacg tcaaaaccaa cgatctggtt gtttgcatca atcgattcaa acggaggata
     2161 ggaggcttcg gtagcaaaac gaatggtttc ggcagctgtg gcggaaagac taaaacctgc
     2221 aattaacgcg gcaatcagaa cttttttcat tgttgttatc ccgaatctta gtgagagaga
     2281 tagtttttaa atgcttcggt ttgcggctcg gtaaagcagc tcgcgtcgcc ttgttctacg
     2341 atatgaccat tttccatata caccactcgg ctggcggttt tacgcgccac ttcaacttcg
     2401 tgggtgacga tcacctgggt aatattcgtt tctgccagct cacgaatgat gctgacgatt
     2461 tgtgccgtaa tttccgggtc cagtgcggcg gtcggttcat cgaacagcag tacctgcggt
     2521 tccatcatca acgcacgggc aatagcaaca cgctgctgct gaccaccaga aagatgcagc
     2581 gggtaacgat cgctataagg tttgagacgc agacgttcca gcagtttttc tgcacgggcc
     2641 agcgcctgat ctttactcaa ccccagtaca cggcagggcg cttcaatcag gttttgctgc
     2701 acggtcagat gcggccacag gttgtattgc tgaaacacca tgccaacgtt acgacgcaaa
     2761 tcgcgaatcg ctttgtcaga gggtgttttg gtaaaatcga aatggttgcc tgcaatgttg
     2821 agcgtaccgg agcgcggcat ctcaagcaga ttgagtacac gcagcagcga gcttttaccc
     2881 gcgccgctgg ggccaagtaa caccagcgtt tcgccctgtg ggcaatccag cgtgatatcg
     2941 aacagcgcct gatgcgcgcc gtagaagcaa ttaatgccgt ttaattgaat actcattgac
     3001 actcgtatac tggcagtctg atagctattg aggtcgaaga tagtaccttt gacagaataa
     3061 ttatgcaata tttctgcttt aaaagttaaa agcaaagcgc attattcaat aaacatagca
     3121 caaaataacg ggggcggtgg tcggtgagca taaatgtcgg cattcctcac gaaatgccgg
     3181 acaatttacg gggtttattg gttgatcaag gcgttagcga ttctcgatgg actgacggag
     3241 cgtacccgcc gtggcatgaa cgctgccgcc taaatagcgt acatcgtcga tcacccaaca
     3301 ctggccttcc tgaatcatta acacttcatc ctgccagctc tgatcaccct gttttagatc
     3361 cacgcgcaat ggaatgttac gggcatcacg attagggata gtcgatgcac tggcaacgtg
     3421 ggcgctgtct ggcaaggtgg ttcgactgga gaatggatcg ttggtcagta gttcccgatg
     3481 gttattatcc cgggaggcat cgctaagcag tgtcgccagt ttgtcgctca gataagggcg
     3541 caaggcggtg atgtcgttgc tgcggtgcaa aatgcggtag tcataaaatt gctgggccac
     3601 gttatctggg cctccttcaa cgcaaggacc actgcgtgtg ccgttatctt tataagctgg
     3661 agtgactgtg gtgcaggcac tgaggagcag tgcgcagggg ataagcattg tcaatttgct
     3721 gtagcgcata atgatttcct tataagcgat cgctctgaaa gcgttctacg ataataatga
     3781 tatcctttca ataatagcgt atcagtccga taatgctttt gagatcgaag gcttaacaaa
     3841 caaggagatc gatcatgcaa ttttctacaa ccccaactct ggaaggccag accatcgttg
     3901 aatattgcgg tgtggtgacc ggcgaagcga ttttaggtgc caatattttc cgtgatttct
     3961 ttgctggtat ccgcgatatc gttggcggac gctccggtgc gtatgaaaaa gaactgcgca
     4021 aagcgcggga gatcgccttt gaggaacttg gctcccaggc ccgggcgctg ggggccgatg
     4081 ccgtcgtcgg tattgatatc gactacgaaa cggtcgggca aaacggcagt atgctgatgg
     4141 ttagcgtcag cggtacggcg gtgaaaacgc gtcgatgaga agagtcttct ggctgatcgc
     4201 tgccgctctg ttactggcag ggtgtgcagg cgaaaaaggc attgtcgaga aagagggata
     4261 tcagcttgat acccgacgcc aggcgcaggc ggcgtatccg cgcattaaag tgctggtgat
     4321 ccactacacc gcagatgatt ttgatagctc actggcgaca ctgaccgata agcaggtcag
     4381 ctcgcattat ctggtccctg cggtaccacc gcgatacaac ggtaaaccgc gcatctggca
     4441 actggtgccg gaacaagaac tggcctggca tgcggggatt agcgcctggc gcggggcaac
     4501 gcgccttaac gacacctcta ttggcattga gctggaaaac cgtggctggc aaaaatcggc
     4561 cggagtgaaa tattttgccc cgtttgaacc ggcacagatt caggcgctta ttccactggc
     4621 gaaagatatt attgcccgtt atcacatcaa gccggaaaac gtagtggcac atgcggatat
     4681 cgcaccgcag cgcaaagacg atccggggcc attatttccc tggcagcaac tggcgcagca
     4741 ggggattggt gcctggccgg atgcgcagcg ggttaacttt taccttgccg ggcgcgcgcc
     4801 gcacacaccg gtagataccg cgtcattgct ggagcttttg gcgcgctacg gttatgacgt
     4861 taaacctgat atgacaccgc gcgagcagcg gcgcgtgatt atggcattcc agatgcattt
     4921 ccgcccgacg ttatataacg gcgaagcgga tgcagaaact caggcgattg ccgaagcatt
     4981 gctggagaaa tacgggcagg attagcgcgg cagttttccg tggtcgcgta gccaggcggc
     5041 agttttctcg ataccttcat ccagggtgat gaccggctga taacctaact cttcctgcgc
     5101 acgcgtaata tccagcgtaa agtcaaaatt caacttggag acgccgtagt gggtcagcgg
     5161 cggctctttt gctgacttgc ggcctaaacg ctccatgctg cgggcgatca tatccagcat
     5221 cgggtagggg acggaacgaa tacgacagtc aatattcaac tcgtcgatca gcttctgcac
     5281 gatgctgcgc agtgtgcgat gctcgccgtt ggtgatgttg tacacacgcc cggaaggtag
     5341 cttatcgcag gcttcctggc ttgccagcca cattgcgtgc acggcatttt catagtaggt
     5401 catatcgacc agcgcactgc cgccatgcgg taacagaata ctgccgtagt ggtgcatcat
     5461 atgcgccaga cggggaataa agactttatc gtgcggtccg aacagacttt gtgggcgcag
     5521 aatagtaaag cgcgtttgtg gattcgcctg cgaaagcata ttgatcactt cttcgctggc
     5581 tgctttgctg cgggcaaact cgttggcgaa gcggtgaggg cgaaaatctt ctttaatatc
     5641 gcgatggtgg tgataatcga agtacaggga gggggaagag atatgaataa agttacgcac
     5701 accccaggcg acagcccatt cacccaggcg gcgagtggcg cgaacgttag ccagatcgaa
     5761 agcctgttgt gtcccccagg gtgaggtaaa gctggagcag tgccacagcg tatcaatgcc
     5821 cgcgagcatc actttagctt gtgatgaaac cagctcggtc agatccgccg gaacaaactc
     5881 tgcgcccatt ttttccagca atttgcccat tgcctcgttg cgaccggtcg ctcgcacgct
     5941 gatgcctttc tggcataaaa actctaccgc gtttcgacct aagccgctgg tggcgccggt
     6001 aaccagtacc ttcatatcaa tccactgttg ttgagaaaat aacgtgcgca ttcttccgtg
     6061 atttccccca tgatgcaatg ggaaacatga aagaataacg caggttttgt cgattaatct
     6121 gtgctttgtt ctgccagtct ggcgatttgt tttgccattc cgcgaaaaat aaacagatgc
     6181 gcggggatca tcaataacca gtaaaacagc cccggcatac cgtgcggatg ccagaaagcg
     6241 cggacatcga tagtacgata gtcgccttta tcttccaggc taaaacacag tcgtcccagc
     6301 cccggcgctt tcatgccaaa taacaacgta agttgttttt ccggttcaac gacaatcact
     6361 ttccagctat ctaccgcatc gccagtctgt aaatattcgc gctccgggcg gcctttcgcc
     6421 agcttatgac cgatcgcgcg gtccatcaac gcccgtgtct gccacaaaat attgccaaag
     6481 aaataacgct ctttaccgcc gatttggttc actacctgcc ataaagcagc aaggctggcg
     6541 gacgttttaa cggtaaaccc cgcctgtttg gcaaaataac cgtactccgg tcgccagcgg
     6601 gcaaaggcct gagcgtcgta gccccagtcg ctggagttga ccagtttttc ctcctctttc
     6661 aacgtgctac gtaccgcgtc atcgaaagcg atcagccgtt gtgggatgag tgcacgtagc
     6721 gcggtatcat ccgccagcag atcgtgtttc agcccctgaa tcaacgccct ggcggtggtg
     6781 ggcggtacgg aagtaatcac attgagaaac cacac
//
LOCUS       DAZGVQ010000229         6785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000229 DAZGVQ010000000
VERSION     DAZGVQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.231"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(16..1413)
                     /gene="ccp"
                     /locus_tag="ACHLAG_004840"
     CDS             complement(16..1413)
                     /gene="ccp"
                     /locus_tag="ACHLAG_004840"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFP0657927.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1818..3467
                     /locus_tag="ACHLAG_004841"
     CDS             1818..3467
                     /locus_tag="ACHLAG_004841"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFP0657928.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGVYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3518..4120)
                     /locus_tag="ACHLAG_004842"
     CDS             complement(3518..4120)
                     /locus_tag="ACHLAG_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP0657929.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4640..5539
                     /locus_tag="ACHLAG_004843"
     CDS             4640..5539
                     /locus_tag="ACHLAG_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0657930.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIAPPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            5588..6601
                     /gene="yhjD"
                     /locus_tag="ACHLAG_004844"
     CDS             5588..6601
                     /gene="yhjD"
                     /locus_tag="ACHLAG_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFP0657931.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
BASE COUNT         1699 a         1761 c         1622 g         1703 t
ORIGIN      
        1 gggcgatcaa attaattatt gtttatcctg catatacggc gtgtacaccc cgttcagact
       61 gtgcaggaaa gctacgatat catccacatc ctcctgcggc agctctttgc ctacctgata
      121 gcgcagcatc agtttcaccg ccccgtccag cgtcggcacg tcaccgcgat ggaagtacgg
      181 tgccgttaaa gcaacgttac gtaaaccggg tactttctga cgcaatttat cacgctcttc
      241 tttagtcaca ttcatacgac caatatccgc cgccgtaatt tccccaaagt taaagtcttt
      301 tttcagcccc aacggttcaa aggaacgtcc gccgagaata ataccaccat gacaagttgc
      361 acatttatta tctttaaata attgatagcc ttttttctgt tgcgccgtca gcgcattttc
      421 atctccacgc aaccatttat caaatgggga atccggcgta attaatgttt tctcaaattc
      481 agcaatggca tcagtaatat tttcgccact gaaaccttgc ggataaactt cgaggaactg
      541 cgctttaagc tgcggatctt tttccagctt agcaataatt tcgtcccagg atttcgacgc
      601 catttcaatc gggttcaacg gcggtccacc tgcctgatcc tgcaatgttg ccgcacgacc
      661 atcccagaac tgctcaacgt taaatactga gttaaatacc gtcggcgcgt taatcggccc
      721 aactgcgcca ccaacaccaa tcgatgtttt tctgccatcg acgccccccg cattcaacgc
      781 atggcaatga gcgcatgaaa tggtgctatc agccgataaa cggggatcgt gatacagcgc
      841 aaagcccaac gccacttttt gcgcatcggt aggcagtttt tgcgggatgg gctgcaccgg
      901 ttcattgcga tgctccggag cagtatcatt gctggcgtaa tattccgcgc gctgttttgc
      961 aatccaggcc agtatttccg cccgctcttc atcactcacc ttacccgccc agtgtagtgc
     1021 ggtataacgc gttggtggca tagtttcata ctgcatcacc cattcaatct tattcagatc
     1081 gctttgcgaa acgggtttat cagccagcag tgccgcacgc acggcctcga ggttaaaaga
     1141 tttatatcca agcttaatgt cgtaatccat caactgtttc gcgccaggaa tataataata
     1201 ggcgggtaat tctgccgaag gtgtgtggca atagtcgcat cctttttcgc ggagaaagcc
     1261 taaaacctta ttattttcac tgacagcaga tgcctgaaca tcggcctgtt tactgcgttt
     1321 attatcgtgg taccacacat aaccagataa ccctaaatag caaatcgcga cgccagccag
     1381 gccgatcgcg gtaatacgtg agaccatttt cattattctt ccctcacggt ggtgagttat
     1441 tgtgacccag aaaattttcc agaatgctgc aaacgtctct gcgagacaaa gccatcataa
     1501 aaaacaggga gatacgcttt ttgataggaa tcaatcaatt acatgaatat aaactatcaa
     1561 taagatagcc tgtgcctatt acccagatca ggaatggcat atcatgattg cggcgagagc
     1621 aggattgaat gttctgaaaa tgaaaaacaa cacgaaatcc gtatttttat aaattccatg
     1681 gatattagtg cgttatattt tttgacaggt taataaacgt gatccaccgc acgctttgtc
     1741 gcccaccagg cggagcgaat gactaccctt aaagaaaagc ccgataatta gcgacgaatt
     1801 tcggaggttg gatccttatg ctcaatcaga aaattcaaaa ccctaatcca gacgaactga
     1861 tgatcgaagt cgatctctgc tatgagctgg acccgtatga attaaaactg gatgagatga
     1921 tcgaggcaga accggaaccc gagatgattg aagggctgcc cgcctctgat gcgctgacgc
     1981 ctgccgatcg ctatctcgaa ctgttcgagc atgttcagtc ggcgaaaatt ttccccgaca
     2041 gtaaaacctt tcccgactgc gcacccaaaa tggacccgct ggatatttta atccgctacc
     2101 gtaaagtgcg ccgtcatcgt gattttgact tgcgcaagtt tgttgaaaat cacttctggc
     2161 tgccggaggt ctactccagc gagtatgtat cggacccgca aaattccctg aaagagcata
     2221 tcgaccagct gtggccggtg ctaacccgcg aaccacagga tcacattccg tggtcttctc
     2281 tgctggcgct accgcagtca tatattgtcc cgggcggccg ttttagcgaa acctactatt
     2341 gggattccta tttcaccatg ctggggctgg cggaaagtgg tcgggaagat ttgctgaaat
     2401 gcatggccga taacttcgcc tggatgatcg aaaactatgg tcacatcccc aacggcaacc
     2461 gcacctatta tttgagccga tcgcaaccac cggtttttgc gctgatggtg gagttgtttg
     2521 aagaagatgg tgtacgcggt gcgcgccgct atctcgacca ccttaaaatg gaatatgcct
     2581 tctggatgga cggtgcagaa tcgttgatcc ctaatcaggc ctatcgccat gttgtgcgga
     2641 tgccggacgg atcgctgctc aaccgttatt gggacgatcg cgacacgccg cgtgacgaat
     2701 cctggcttga ggacgttgaa accgcgaaac attctggtcg cccgcccaac gaggtgtacc
     2761 gcgatttacg cgcgggagcg gcctcaggtt gggattactc ttcccgttgg ctgcgtgata
     2821 ctggtcgtct ggcgagcatt cgtaccaccc agttcatccc catcgatctg aatgccttcc
     2881 tgtttaaact ggagagcgcc atcgccaaca tctcggcgct gaaaggcgag aaagagacag
     2941 aagcgctgtt ccgccagaag gccagtgccc gtcgcgatgc ggtaaaccgt tacctctggg
     3001 atgatgaaaa cggcgtctac cgcgattacg actggcgacg cgaacaactg gcgctgtttt
     3061 ccgctgccgc cattgtgccg ctctatgtcg gcatggcgaa ccatgaacag gccgatcgtc
     3121 tggcaaacgc cgtacgcagc cggttactga cacctggcgg gattctggca agcgagtacg
     3181 aaaccggtga acagtgggat aaacccaatg gctgggcacc gttacaatgg atggcaattc
     3241 agggatttaa aatgtatggc gatgaccttc tgggtgatga aatcgcgcgc agctggctga
     3301 aaacggtgaa tcagttctat ctggaacagc acaaaatgat cgagaaatac catattgccg
     3361 atggtgttcc ccgcgaaggc ggcggtggcg agtatccgtt gcaggatggg tttggctgga
     3421 ctaacggtgt ggtacgccgt ttaattggtt tgtacggcga accataatat ttttacagcc
     3481 agccgctaac ttcctgctgg ctgtaaaatt atcctcttca ggaggagata tttaacatca
     3541 ttgccgcctg ggtgcgattt ttcacttcca gacggcgata cagggattcc agatgcgctt
     3601 ttaccgttcc ggtactgata ttcagtgctc tgccgatctc cttatttgat tcgcccgccg
     3661 ctaacatggt taaaatctcc cgctggcggg cgcttaacga tttgagatct ttaatgtcct
     3721 tttccggcgt cgtccgccag tctccaggca gaaacatcat ccccatcgcc gcactattta
     3781 ccgccaacgc aaatgtctcg acggttgaat cacgaggcac aatggccagc acattaaaat
     3841 ggataacttc ctgtaaccac cgtttattgc aatccgtcgc cgtgattaac accttaacct
     3901 caggaaactg caccacggtt ttttgcagca accagtagca aaactcacca tcctgatcgc
     3961 catcgagcat aactaaggct tcagggtaac tttccagctt ttgccataac tcgtctgcct
     4021 gactggcccc ctgaatactc actcctggaa tacgctgctg taaactgatt ttcattccat
     4081 gaataaatat tgactgcctg tcaaacatga ctatttgcat aactgaatct ccacctgaat
     4141 acgttaaaaa gacttaagta gtggaagggt attacccgcg agaaaaaata agaattcgcc
     4201 atttggcggt ggccattcta cagagatgac gcgtagaaaa tagttaccga tataaatagt
     4261 tacagctaaa cgcctgaaat tacatgtcga gggcactatt taaaacaatt ttgaggattt
     4321 ccttatattg gtggttagta cgcatgcaat taaaaatgaa actccgcgac cacaagcaaa
     4381 aataacaaac ggcaatgaga caaaaataag cacaaatagc caacacgtcc tctattcact
     4441 ttaaagggaa tcgctgaaaa atacgctctg tttaggggga ttcacctttc tcagaaagct
     4501 attccgccct tttcctgctg agaaatcgcc acattcgtca tgacaacatt gtgaaacccg
     4561 gcattagatg ttagaaaaaa caataaacaa tgcgatatgc gcgttactgt ttcgctgtga
     4621 acaacaatcg gtcaaagaaa tggataaaat tcacgcaatg cagttgttca tcaaagtcgc
     4681 ggagctggaa agtttttccc gcgcggcgga tttctttgct ttgccaaaag gaagtgtttc
     4741 gcgccagata caggcactgg aacatcaact tggcacccag cttctccagc gcaccacgcg
     4801 acgggtcaaa ctcacgccag aaggcatgac ctattatcaa cgagcaaaag atgtgttgag
     4861 taatctcagc gaactggacg gtctgtttca acaggatgcc accagtatca gcggtaaatt
     4921 acgcatcgac atcccgccag gaatcgcgaa aagcctgtta ctgccgcgcc tgtcggaatt
     4981 tctctatctg catccgggaa ttgagctgga actgagtagc catgaccgtc cggtagatat
     5041 tcttcatgat ggttttgatt gcgtgatacg cactggcgcg ttaccggaag atggcgttat
     5101 cgcccctccc ctcggcaaac tgaccatggt caactgtgcc agtccgcact atctgacgcg
     5161 ctttggttat ccgcaaagcc ccgatgatct gacttcacac gcaatagtgc gttatacacc
     5221 gcacctgggt gtacatccgt taggttttga ggttgccagc gttaatggcg tccagtggtt
     5281 taagtctggc ggcatgttga cggtaaacag tagcgaaaac tatctcgccg ccggtcttgc
     5341 cggtctgggg attattcaga tcccgcgcat tgccgtgcgc gaagccctgc gtgccgggcg
     5401 gcttattgaa gtgttgccag gctaccgtgc cgagccgctc tccctttcgc tggtttaccc
     5461 gcagcgtcgg gagctttccc ggcgtgtaaa cctgtttatg cagtggctgg ctggcgtaat
     5521 gaaagagcac ctggactgac cgactatact ttttaagaat gaccacgaca gaaggacaaa
     5581 agagcggatg acgcaggaaa acgagatcaa acgtcccacc caggatctgg agcacgagcc
     5641 gattaaacag ttggataata gcgagaaagg cggcaaagtt agccaggcgc tggaaaccgt
     5701 caccaccact gccgaaaaag tccaacgcca accggttatt gcgcacctga ttcgtgcgac
     5761 agaacgcttt aacgatcggc tgggtaacca gtttggtgcg gctatcacct atttctcatt
     5821 tttgtcgatg atcccgattt tgatggtgtc gtttgccgca gggggctttg tgctggcctc
     5881 ccatccgatg ttgctacagg atatcttcga caaaattctg caaaacatca gcgatccgac
     5941 gctagccgcc acgttgaaaa acaccatcaa caccgccgtt cagcagcgta cgactgtagg
     6001 gcttgtcggc ctggcggtgg cactttattc cggcatcaac tggatgggta acctgcgtga
     6061 agcgattcgt gcccagtcgc gcgatgtctg ggaacgctcg ccgcaagatc aggagaagtt
     6121 ctgggtaaaa tatctgcgtg attttatttc actgattggt ttgttaattg cgctgattgt
     6181 gacgctgtcg atcacctcgg ttgccggttc ggcgcagcaa atgattatta gcgccctgca
     6241 cctgaacagc attgagtggc tgaaaccgac gtggcggttg attggcctgg cgatttccat
     6301 cttcgccaac tatctgcttt tcttctggat cttctggcgg ttgccgcgcc accgtccgcg
     6361 caaaaaagcc ctgattcgcg gaacatttct ggctgctatt ggttttgaag tgattaaaat
     6421 cgtgatgacc tacaccttgc catcgctgat gaaatccccc tctggcgcag catttggttc
     6481 cgtgctgggg ctgatggcat ttttctactt cttcgcccgt ttgacgctgt tttgcgcggc
     6541 gtggattgcc accgccgaat ataaagacga cccgcgaatg ccagggaaaa cgcagcctta
     6601 aaataggccg gatgcggcgt aaacgcctta tccggcatac gattttattc ggcctggctc
     6661 cccgtaggcc ggataagatg cgccagcatc gcatccggct ataatgcgca cataacctct
     6721 tgaaactctt ccccagagcc tcttcagcca tctatttggg agcaaacaat ttcattccaa
     6781 ctcat
//
LOCUS       DAZGVQ010000230         6782 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000230 DAZGVQ010000000
VERSION     DAZGVQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.232"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="ACHLAG_004845"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="ACHLAG_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFP0657932.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="ACHLAG_004846"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="ACHLAG_004846"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFP0657933.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="ACHLAG_004847"
     CDS             complement(941..1408)
                     /locus_tag="ACHLAG_004847"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP0657934.1"
                     /translation="MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGCDSLLAGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHLAG_004848"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHLAG_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFP0657935.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="ACHLAG_004849"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="ACHLAG_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFP0657936.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="ACHLAG_004850"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="ACHLAG_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFP0657937.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="ACHLAG_004851"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="ACHLAG_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFP0657938.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="ACHLAG_004852"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="ACHLAG_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFP0657939.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHLAG_004853"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHLAG_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFP0657940.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="ACHLAG_004854"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="ACHLAG_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFP0657941.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHLAG_004855"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHLAG_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFP0657942.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="ACHLAG_004856"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="ACHLAG_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFP0657943.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHLAG_004857"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHLAG_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFP0657944.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHLAG_004858"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHLAG_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFP0657945.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
BASE COUNT         1847 a         1594 c         1775 g         1566 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctc cgtgaggcta
      781 tcgcgtatgc cgacagcgtt catgactatg ttagccgtga tatgatgatc gagatccttg
      841 ccgatgaaga aggtcatatt gactggctcg aaactgagct ggatctcatc ggtaaaatcg
      901 gtttacaaaa ttacctgcaa tcacaaataa aagttaaaga ttaacggcct gctagcaaac
      961 tatcgcaacc tacaacgaaa cccgcagccg ccagaaatgg cccaaacggc agcgggtttt
     1021 ttaatgactc ttttcctctc atgagcagac caacaactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggcaagaagg tccaggtatg ccaggcacca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgacg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaataa tcgcccccca taacgcatcg gctaaaccgt
     1261 caggattaca aacctgagag aacaaaagac ctgaccagag taacgggcag gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccata aaaaaagtag gacagataaa caagcgtaca
     1381 aaatcagaaa gggaaggtta gcgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagaa atcgctgatt tgttaacctg gttccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatac agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aa
//
LOCUS       DAZGVQ010000231         6664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000231 DAZGVQ010000000
VERSION     DAZGVQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.233"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            247..888
                     /locus_tag="ACHLAG_004859"
     CDS             247..888
                     /locus_tag="ACHLAG_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657946.1"
                     /translation="MNVENNQLGGSEWLQTGKAREHGADVLSCSENPSGEACKRGQDE
                     NKAYAAALATSSVSLLPRSAQAMWALGAGANAGIGSIVYSSVDPENAIIAGWVNVITM
                     GQGWKGTVGWNAAGGAFGNWLDGKDPLSGAAMSGGTSAIGYGVGKVIQGPLDKLINPN
                     WKNWEWLNVGMGISKPLPLNPTPGIAGNIGSSIGSEGSGAIIQEQLKNQQEKK"
     gene            1202..1804
                     /locus_tag="ACHLAG_004860"
     CDS             1202..1804
                     /locus_tag="ACHLAG_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFP0657947.1"
                     /translation="MVSYGQAVASWNQYADANNLTPEQKQAGLDKLAKGELPEGANIS
                     KVIVDGYKDGVLIAGAWYLGPAASTGKVVSGAVISAIANGTYQWYDLSKPGNENKSWD
                     YWGSASAGITGALAPGRNMWQNAGIAAGGTLFTDGPDKGALAGTGAGWVFGTVVGVVA
                     PPVFSPVLGPGSVPSGDIIGAIGGEFISNAVKDEINGTKK"
     gene            2718..3293
                     /locus_tag="ACHLAG_004861"
     CDS             2718..3293
                     /locus_tag="ACHLAG_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family protein"
                     /protein_id="HFP0657948.1"
                     /translation="MKKLVIISVMILSVMSGSTLADNHTLSAGYAQSKVQDFKNIKGV
                     NLQYRYEWDYPVSVVGSFSYMKGDWADSHRDEADDFYRQQADIKYYSFLAGPAYRLND
                     YISFYGLVGISHTKAKGDYEWRNSVGADESDGYLSESVSKKSTDFAYAVGVIINPWGN
                     MSVNVGYEGTKADIYGKHSVNGFTVGVGYRF"
     gene            3471..5216
                     /locus_tag="ACHLAG_004862"
     CDS             3471..5216
                     /locus_tag="ACHLAG_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP0657949.1"
                     /translation="MTPGERRVASRLESFLNDDCFVWYDIPVGRKNRHPDFVIIDPDN
                     GLVFLEVKDWTVSTLRKANQEQVTLETDGLLKSEINPLVQVRRYACDTVNALPADPCL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKTLAGNDENAVEKIFPSAQTICQDEMTQS
                     VLPEIFRQKIAGMFTTGFRTRVTPRMRDILRAHLFPEVTVKQNSQIKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTSGKILVLCYNITLASYLRECIEARGL
                     RSRVAVSHFHSWCASMAKRHGIQVTADRKEYPEKCFSALEEAVNSGKITQTGYDAVLV
                     DEGHDFDSRWLALIARLFDNVSRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNPKRILHFAYAFSREYFEKHQNREIPLVQPQAGGEEGTEPEILRCASVRDE
                     ARQVADWLEKRHALCGHWSDMAVLCPAEFSVKQLKEVMTQRGIPFATCFDSEGKKAYS
                     RRKDVVHLLTYQSSKGLEFPYVAVINASFVPSGVADESEVIPVLYVAFTRATRELLVT
                     CYRENSISRHLEDFA"
     gene            complement(5390..5677)
                     /locus_tag="ACHLAG_004863"
                     /pseudo
     CDS             complement(5390..5677)
                     /locus_tag="ACHLAG_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(5727..6074)
                     /gene="tnpB"
                     /locus_tag="ACHLAG_004864"
     CDS             complement(5727..6074)
                     /gene="tnpB"
                     /locus_tag="ACHLAG_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP0657950.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGNVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(6071..6451)
                     /locus_tag="ACHLAG_004865"
     CDS             complement(6071..6451)
                     /locus_tag="ACHLAG_004865"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP0657951.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPLLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLHFNGNVSEKLLTLLIQELKR"
BASE COUNT         1726 a         1295 c         1749 g         1894 t
ORIGIN      
        1 gggctgttta ttttggtgtt attaccgggt taactgcatg ggttgggatt gttttttcta
       61 aactgatagc cagatataaa tcaaggaaaa caccgcctga ctctgagtga cttttgatat
      121 aacgcagaca gtacagttgc aaacatctgt tgggttccag acataacgca aattatgctt
      181 ggttcgttgt tctgaactat acataatttg cgttatgttg aaccgttgga ggctgttcac
      241 ctgtaggtga atgttgagaa taaccagctg ggtggcagtg aatggctcca gacgggaaaa
      301 gccagagagc atggtgctga tgttctctcc tgtagtgaaa atccttccgg tgaagcctgt
      361 aagcggggac aggatgaaaa taaagcttat gctgccgcat tagcgacaag tagtgtttcg
      421 ctgttaccta gaagcgcaca agctatgtgg gctcttggcg caggtgcaaa tgcaggaatt
      481 ggttccatag tatatagttc agttgatcct gaaaatgcaa tcattgcagg atgggtcaat
      541 gtaatcacca tggggcaggg ctggaaagga accgttggat ggaatgcagc cggtggtgca
      601 tttggtaact ggctggatgg gaaggatcca ttatctggtg ctgcgatgag tggtggtaca
      661 tcggcaatag gttatggtgt aggtaaggtt attcaagggc cactggataa attaattaat
      721 cctaactgga aaaactggga atggctcaat gttggtatgg ggatcagcaa gcctctacca
      781 ctaaatccaa cgcctggaat tgctgggaat ataggttctt caattggttc agaaggatca
      841 ggagctataa tccaagaaca gttaaaaaat caacaggaga agaaataatg aagtgtcaat
      901 ctctccttat gcgtttttta gtgactgtac ttatatggtt tattgccatg tttatcatgt
      961 cggtaatgat atttttcttc atcagagtgt ttgcatttta ttggattggt ggcgattttt
     1021 tagtctcata tgttgatgtt atgaaatctt taaaaatagc agtgtattgc tctctgctat
     1081 gtagcatagg gagttgggtc ttatatcgaa gtaataaaaa aacaatagat aatagaatta
     1141 atcccgtcct taatccggcg ggattttagt tgagaataat gcgctcagtc tgccatcagg
     1201 aatggtgagt tatggtcagg cggttgcctc ctggaatcag tatgctgatg ccaataatct
     1261 caccccggaa cagaagcagg caggactgga taaacttgcc aaaggagagt tacctgaagg
     1321 tgccaatata agtaaagtga tagttgatgg ctataaagat ggcgtattga ttgcaggtgc
     1381 ctggtatttg ggacctgctg catcaacagg taaagtagtc agtggcgcag tcatctcagc
     1441 cattgccaat ggtacgtatc agtggtacga cctgagcaaa ccaggtaatg aaaacaagag
     1501 ctgggattac tggggaagtg cctcggcagg cattaccgga gcactggctc caggtcgaaa
     1561 tatgtggcag aacgcaggga tagcggcagg tggaacccta tttactgatg gacctgataa
     1621 aggtgcttta gctggtacgg gagctggttg ggtattcggt actgtagttg gggtagttgc
     1681 accgccggta tttagccctg ttttaggtcc aggttcagtg ccttcaggtg atattattgg
     1741 tgctataggt ggtgaattta tcagcaatgc agttaaagat gagataaatg gaacgaaaaa
     1801 ataaagctac tgtaggttta ctgctcctaa gtattatgta catgattttc tgctgcttaa
     1861 tattcttctc cttgactctg atagtagtta agtttgttat tagccatgaa gtgaatatta
     1921 agcaatcaga tcttgaaaat attttagttg ctagtgttat tgttggaact gcagcagcat
     1981 ttagatcgtg gatcttcacc aaattggacg aacgcaagac tcgaaaaacg ccctcgtcag
     2041 gatcctgagt aatccggaca ttgggaaata gcgttcttgc tggtgccgca ggtgctgcca
     2101 ccggcgaggt ggtggggatg atagccacag agatgtgcgg aatatcccct ggtactgtta
     2161 catgtaaaag atatataacg aagctacatc cttctcttta tgaacggatt tatattagtt
     2221 ttgctgacaa gattgagata aattcggcca atgaagaaga atagggtatt ctttaaattt
     2281 ctgataacat gtgacgcatt ttaaatattc aggcaggaag aattaataat tttcctaatt
     2341 atggaccgac tgatatagca aagtaaatat tctataggaa atcctggata ttatgaataa
     2401 tgtattaagc gtattgaaat gatgatgtag gcgaagactc agtctggtga gttacattat
     2461 gtcattgagg caggatttaa cgatacaggg ctggttcgtt atggaacaac atttatacct
     2521 gaggaaaggg gctgtggtat ctgaggcaac agaagtttgt ggggagttcg gtttattacc
     2581 atgcttggtt ttttgtcagt gtgttcaaat cgtcagatga tatatttata gttttttgat
     2641 gatgatttca aataatcttt tgtgatgtgt ggcccttttt gattttcaga aaccatttat
     2701 taatgaagga gggttaaatg aaaaaactcg ttataatttc agtaatgata ttatctgtta
     2761 tgagtggttc aacactggct gataatcata cgctatcggc tggttatgct cagagtaagg
     2821 tacaggactt taagaatatt aaaggagtga atctgcaata tcgatatgag tgggattatc
     2881 cggtcagtgt tgtcgggagt ttttcgtata tgaaaggtga ctgggcggac agtcatcgcg
     2941 atgaagctga cgatttttac agacagcagg ctgatattaa atattattca tttctcgcag
     3001 gcccagctta tcgtctgaat gactatatca gtttttatgg tttggtgggg atatctcata
     3061 ctaaagcgaa gggagattat gagtggagaa acagtgtggg tgctgatgag tctgatggat
     3121 atttaagtga gtctgtatca aaaaaatcca ctgattttgc ttatgcagtt ggtgttatta
     3181 ttaatccgtg gggcaatatg tctgtaaatg ttgggtatga gggaactaaa gcagatatat
     3241 atggaaaaca ttcggtcaat ggatttactg ttggagttgg ttatcgtttt taattattag
     3301 acgggactgg ataattgcac ttgcgtagtg aagtttactg aatgtttgta ttttaatgaa
     3361 gtattcgaca tattcccttc agacggaata tgtcgacggt tatgatttta ctggagcatg
     3421 agtatgttat ggcgaaactg ataaatatat cagcgtccac tgaggagaga atgacgccag
     3481 gggagaggag ggtggcttca cgactggaga gctttctgaa tgatgattgc tttgtctggt
     3541 acgatatccc ggtgggacgg aaaaaccgac atccggattt tgtcattatt gacccggata
     3601 acgggctggt ctttctggag gtcaaggact ggactgtatc cacattacgt aaagcaaatc
     3661 aggagcaggt tacactggaa accgacggtc tcctgaaatc agaaataaat ccgctggtac
     3721 aggttcgtcg ttatgcctgt gatactgtta atgccttacc tgcagacccg tgcttacggc
     3781 agaatgacgg ccagtataag ggacggctga acctggcctg ggcgtatggt gtggtgttta
     3841 cccgcattac ccgtcagcaa ctgaagacac tggcagggaa tgatgagaat gcggtggaaa
     3901 agattttccc ttcagcacag acaatctgtc aggatgaaat gacgcagtct gttttacctg
     3961 agatattccg gcaaaaaatt gccgggatgt ttaccacggg gttcagaacc cgggtgacac
     4021 cccggatgag agatattctg cgcgcccatc tgtttcctga agtgaccgtg aaacagaact
     4081 cacaaatcaa aatcatggat atccagcagg agattctggc cagaaatatt ggtgacggac
     4141 atcgtgtcat tcatggtgtg gccggttccg gaaaaaccat gattcttttg ttccgttgcc
     4201 tttacctggc agaaaccacg tccggaaaga ttctggtgtt gtgctacaac attaccctgg
     4261 ccagctatct gcgggaatgc attgaggcca gagggctgag gtcacgggtt gcggtatctc
     4321 attttcacag ctggtgtgcc tcaatggcaa aacgccacgg tattcaggtg acggcagaca
     4381 gaaaagaata tcctgagaaa tgcttcagtg cactggaaga ggccgtgaac agcggaaaaa
     4441 tcacccaaac cggatatgat gctgtactgg tggatgaagg gcatgatttt gacagtcgct
     4501 ggctggccct gattgcccgt ctgtttgata acgtatcccg ttcactgctc ctgatgtatg
     4561 atgatgccca gtccatttac cgacgggagc gggccctgaa cttttctctg gccagtgtgg
     4621 ggattcaggc acagggacgt acttctgttc tgccggtcaa ttatcgtaat ccgaaacgca
     4681 ttctgcactt tgcttatgca ttctcccgcg agtattttga aaaacatcag aaccgtgaaa
     4741 taccactcgt tcagccacag gcgggtggtg aagagggcac agagccggaa atcctgcgtt
     4801 gtgcttctgt acgtgatgaa gccagacagg ttgcagactg gctggagaag cgccatgccc
     4861 tgtgcggtca ctggagtgat atggctgttc tgtgtccggc agagttttca gtgaaacaac
     4921 tgaaagaggt tatgacgcaa cgcgggatac cgtttgccac ctgctttgac agtgaaggta
     4981 agaaggcata ctcgcgacgt aaggatgtgg tgcatttact gacataccag agcagtaagg
     5041 ggctggagtt cccgtatgtg gcggttatca atgcttcttt cgttccttct ggtgtagcgg
     5101 atgagtctga agtgattccg gtgctttatg tggcgtttac ccgggcaacc cgtgaattac
     5161 tggtgacctg ttaccgtgaa aacagtatca gtcgtcacct tgaggatttt gcataatacc
     5221 ggaccatttc tttatttctg actttcacca tactgttatt atcaggttta tttaattcac
     5281 catgacccgt tatttcacga tattcagatg cgtctttttt atcttaatca tttcccgtat
     5341 tcctggcact ggaatgtcag attcagaccc caggaagcgg tctatattag gtctgacgca
     5401 acggacgctg tacagccggg tcatacaccc taccagtcag cgtatcgctc tctttctgaa
     5461 gccggttcag atcggcttcc atttgtgcga tacggcggga gactttttcg gaacgactgt
     5521 cgaagttcat ccggcggagt ttatccagct gcgcctgcag atggtctatt tcgcgctccc
     5581 ggttgctcag cttttcctgc agggcgtgga tcagcgcttc ctgttcggcc aggcgctgtt
     5641 tcagcaggaa gatgtcgtca gaagagatgt cgttcataag cccgtatttt accgggctta
     5701 ttctgtgaca accaggataa agagatttac agcatggtca gggaggtcag caaccgcttg
     5761 ggctgtcgcc agtcgatacc ttccagcagc atcgccagct gcgcctgcgt aaggaacacg
     5821 ttgccatcac gggctgacgg ccaggcgaag cgcccacgct ccagccgttt ggtcaggagg
     5881 cacagtccgt caccggtgga ccacagcagt ttaacctgac tgccgctgcg gccccggaaa
     5941 atgaaaacat ggccggacat gggatcgtct ttcagcgccg tttgtacttt cgcagccagg
     6001 ccgttgaagc catttctcat atcggtgata ccggcaacca gccaaatttt ggtcccggaa
     6061 ggtaacggga tcatcgcttc agttcctgta tcagcagagt caggagcttt tcgctgacat
     6121 tgccattgaa gtggagcgtc ccgtgccgga acgttacctc acagctgata ctgagggttt
     6181 ccgggtcctc tgcgagcgat tctggctgtt cggcagctgc atcgagagtc acaggaagta
     6241 gcagggggct ctctgaagaa ggtaatagca gctttccctc gcgccattgt tgtcgccatt
     6301 tgaacaacag attggcgtta atgccatttt caagagcaag ttttgagatg gatatcccgg
     6361 gttcacagga ggcagcaacg agctgctgtt taaattcggg aggataatta gggcagcctt
     6421 ttcgcctgcc gggagtcaca tttttctgca tatctgacac tttggttccc actacttatt
     6481 tggtggacac cactttgtct aattcgtcag attctgacca gacggttcag gctgtacgct
     6541 tacggttgtc gtgaaggagg gggaagacca tctcatcgct tactgatgtt cctgttacta
     6601 tatctattga atcctggcta taagttcgct gtttaaagaa ttatatgaat agcaccaatt
     6661 aata
//
LOCUS       DAZGVQ010000232         6615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000232 DAZGVQ010000000
VERSION     DAZGVQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.234"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..519
                     /locus_tag="ACHLAG_004866"
                     /pseudo
     CDS             1..519
                     /locus_tag="ACHLAG_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(762..1457)
                     /locus_tag="ACHLAG_004867"
     CDS             complement(762..1457)
                     /locus_tag="ACHLAG_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFP0657952.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPCVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1450..2877)
                     /locus_tag="ACHLAG_004868"
     CDS             complement(1450..2877)
                     /locus_tag="ACHLAG_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFP0657953.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2888..3607)
                     /locus_tag="ACHLAG_004869"
     CDS             complement(2888..3607)
                     /locus_tag="ACHLAG_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFP0657954.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAVRMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4134..4988)
                     /gene="rclR"
                     /locus_tag="ACHLAG_004870"
     CDS             complement(4134..4988)
                     /gene="rclR"
                     /locus_tag="ACHLAG_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFP0657955.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5214..6539
                     /gene="rclA"
                     /locus_tag="ACHLAG_004871"
     CDS             5214..6539
                     /gene="rclA"
                     /locus_tag="ACHLAG_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFP0657956.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         1798 a         1642 c         1442 g         1733 t
ORIGIN      
        1 tacagcctaa aaataaacca gacaacacca ggcatacttg ttaactgtga tagcaataat
       61 aactttgcct gtcagaccgc aatgactgaa gatgttatac agcgaattac tacatttttt
      121 cacaccagcc cagatgtcaa gaacagagaa ataaagctgg aatggtcatg agataagaga
      181 gatttaccaa ctgctgaagc ggaaatatct cgcgtgtagg cgtctattat caaatggtat
      241 gcgtcagaat accataatgg caggcaagtt ctcgatgaga tacaaatacc ttcagcaatt
      301 aacagtgagc tttatacaaa aatgatatac ctgaccagga actggtcatt gtatccgaac
      361 ggtgatggct gtgtaactat aagctcacca gaaataaaaa ataaataccc tgctgccatt
      421 tgcctggctc tgggattttt tctaagcatt gtaatttctg taatgttttg ccttgtcaaa
      481 aaaatggtag atgaatacca acaaaactct gggcagtaat acacagcatt ctgatagaga
      541 gtgcctgtat aaagctgaag tgacaatgac cgggaaggga acccgttttc aggaatggat
      601 gcctgtcttt ttcactgtaa atatcctggc attactaatg ccaggatatt tacagaagag
      661 cagcataacc atgacggaac ggggttaagt caggttgtcc ttaacgctat tttcaacctc
      721 gtatgccttc ttcaggttta tgttcagatc tcatatctct ctcagcaatc ctcaataatc
      781 agatacaccg ctttcaccgg gccatgaacc ccgacgactt tgataagctc aatatccgcc
      841 gttgaactag ggccgctaat gatgttaatg caggacggca ttcgttcacc ggcctgcgct
      901 ttctgatgca atttttctgc gagttgcgct acacacggca ggattgtgct tttacgcagg
      961 ataaaaagag aagattccgg gagcaggctc aatgaacgcc cgcgcccggc ggcggaaaaa
     1021 agaaccacgc ctcccgattc ggttaaacca tattcagcat acacgacacc cactttagcc
     1081 tgctcggcct gcgagatatt ctcggcacct ttcgccggat cccaaacaac ggcattgcat
     1141 tcctgctgca aacgttcact aatccccaat tcctccagcc tcgtgtcacc gctaatcacg
     1201 accgactgat ctcccagctc tttacacaga cgtattgcag cttctgccgc cttcgcctcg
     1261 ctggtcagct cacagcgcgt caacataaca tcgctggcaa actgaataaa cgcgtcacag
     1321 cgctgctgtt ggttaagttg ggtaagccgc tcgttagcat agttgttaag cggcgcatct
     1381 tctgcttgcg gttcaagtcg cagcgggcga cccagtgcct gagcaacgtt attcaaaaat
     1441 tcgcctcgat tatccattct ttttctcctg cgcctgatgt ttcttaaacc aactacggaa
     1501 actctctccg tcagcttcag gaagatcgcg tgcttccatc cagtcgctaa tcgcgccaaa
     1561 tttgagtggt gttttgccgc cattgataaa ccagcttgcc gcatgagcgc cggccatcat
     1621 cccgactttc cacaatcctg gatgactatt ggcataagcg aacattttta tcgcccgttg
     1681 ctctgctttt gcggtgatcc ctttttcagc catcacccga cgatgacgca aaatcagttt
     1741 tgacagcgga atacgcaccg gacacacgct gtcacaagca gtgcataaag agcaggcgta
     1801 gggtaaatct ttaaaatctt tatagccgcc aagtagcgga gaaatcaccg caccaattgg
     1861 ccctggataa atagagccat atccatgacc gccaatatgg cgatatgccg gacaagtatt
     1921 catacaagcc ccgcagcgaa tacagcgcag cacatcccga aattcagagg ccagcacctc
     1981 agaacgcccg ttatcgacaa taaccagatg aaactcttca ggaccatcaa cgtgccccgc
     2041 ttcgcgcggt cctgtcagcc aggtgttgta tcccgtcaaa cgtgcaccaa cggcactgcg
     2101 cgccagcatg gtgatcaata catctacctc ggcaaacgtg ggggcaatac gctccattcc
     2161 catcactgca atatgcgttt taggcagcgt ggtacacatt cgcgcattac cttcattggt
     2221 caccaggcat accgaacctg tctctgccac cgcgaaatta cagccggtaa tacctatttc
     2281 agcactgagg aaatcttcgc ggattttttg ccggatgaat aaggtcatcg cttcaggcgt
     2341 ttccggcccc tcatagccca gacgttcgtg tagcactcga cggatctgat ggcgatcttt
     2401 atgaattgcc gggaccacaa catgagatgg cggatcttga tccagctgga gaatatattc
     2461 acccagatcg gtttcaatca cctgaatgcc agcatcctgc aacacatgat tgaccccaat
     2521 ctcttcggtc accatcgatt tagatttcac caccttccgg gcatttttgc gttgggcaac
     2581 ctgtaaaatg tagcgggtag cgtcttcttt ggtttttgca aaccagacgt gaccgccgtt
     2641 ttgcgtcact ttttctgaga gctggtacag ataagcgtcg agattactca gaacatgatc
     2701 acgtatctgg gcggcccgat cgcgccactc ctcccagtgc cccaattcat cgaccatttt
     2761 ttgccgattt gccccaatac gctgctgcgc gtttgccacc gctttgcgca tgatcggatc
     2821 ttcaatttgc tgacggatgc gtgtcttaaa atctgtatta ctggttttga tcgacatctt
     2881 tatatcctca gcggctcatc aacacttcag caatatgcat cactttgact ttctgccctt
     2941 cccgttgtaa tcgcccactg atgttcagca ggcaactcac gtcagcacca attaaatact
     3001 cagggcggac ttccatcagg tgcacaacct tttctttcac catctcgccg gatatttcgg
     3061 ccattttgac cgagaacgtg ccgccaaatc cgcagcaggt atcctgttca gcaaaggtca
     3121 acagctccag tccacgcaca tttttcagca gcgtaagtgg ctcgtccttc actcccagct
     3181 tacgggccag gctacaagat gggtgataca cagctctccc ttgcaaactg gcacctacat
     3241 cgactacccc taatttatta acaataaaag aggtgagatc ctgcatacgc acggcaacct
     3301 tttcggcacg tgatgcccat tcaggttcat ccgccagata cgtcgggtaa ctttttacgg
     3361 cataggtgca agagccagcc ggtgaaataa tgggatcgtc gttatcctcc agtgcggcga
     3421 tcagattttt catccctgga atcgcttctt tgatataacc gctattgatc gcaggctgac
     3481 cgcagcatcc ctgtttctcc gggaaattta cgcgacagcc gagtttttcc agtagcagca
     3541 cggagtctcg tgccattctt gatttcaggg cgtcaccaat acaggtgaca aagaaattga
     3601 cattcacaac taatactcca ttacttcatg cccatttatg cccgatgttt cccccccacc
     3661 gggggatcaa tcatcaataa atatggcgtt cttttttaat tggctagcaa cataccaccc
     3721 ctcatttgta tgacaaatga taatctttca tccaaacttc aaatgcaata actaatttta
     3781 tttctttcgg catcgctcac attttatggg gaaatcgacg ttcatttttg gcataagaaa
     3841 tccatttaaa aaggctacat cccttatacc tattcagagc tacttattca ggggttattg
     3901 agatatacac tgatggtgta caaaaaaatc accgacaata atcacgtatc aagtaatagt
     3961 cggggtttta tttaattgaa gcaatttagt ttgcattttt atttaaaaac tgagctagag
     4021 tccatcagaa aaaaatatat aaagcccaaa ccaccgttgg aaatagaaat agttgaaagg
     4081 ctcacaactc ccggaatgcg gattatcttt gccctgattt ctggcggttt tattcagggt
     4141 gcaagctgtc tgaccctttc ccgatattct cccggagtac aaccaaactc gcggacaaac
     4201 gccttgtgaa aagatgattc actggcatag cctactgact cagcgatcac cacaacaggg
     4261 agcatttccc gggaaaacat ctgggccgct atttgtagac gcaactttgt taatacagcc
     4321 agcggcgtgg ttccggaaac atcacggaaa agctgggcaa aacttgcccg ggacatgtga
     4381 gcgatgctgg ccagcgattc gacggtccag gcgtgtcctg gcatttccag catttgctgt
     4441 attaccgcac caagacgtgg atgcagaagc aaactgagaa tgtttttctc agtattaacc
     4501 tgtgcaatcc actcacgcac cgcgagggta aagaatgtag cgcagatctg gctacacagt
     4561 gcatccactc ccggcatcgc cgatctggat tcctgttgta aaaacgggat cgcctccctc
     4621 agccagttat attccacgct gtggttaacc ggtgctaaaa acagcgtttc cggcaaagac
     4681 gttaaaaaat aacgcgccga atgttgcaac cgaagagtgc cgcagacaat acaggtcgat
     4741 tcattatcga catgactcag gcgatgagct gaattttgtg gtagcaggac cacatttccc
     4801 gggcgtaatg taaaaatctc tcccgtcggc atttccagct tcgccgctcc ttgcgttaac
     4861 gcatgccaac gaataaccga taattccccg gcaccatgtg gaagctgcca gtcacttcct
     4921 aacacgcaat tcttatcgat cgttccttgt ggagcgttaa gcatcaacaa acggctaagg
     4981 gcatccatat cagactcctg agtggttttt gattacaaac accaacaatc ttagtaaata
     5041 actaatctaa taccatcaaa tggttaatca tgatttttgt tttgtgattc tgattaagaa
     5101 aaagaaaata ccagacgata agacaaaata tcaagcgttg tgaagaaatg gtatttgctc
     5161 ttttgcgtct atagtcatga tgtcaaatga acgcgtttcg acaggaaatc atcatgaata
     5221 aatatcaggc agttattatt ggttttggca aggctggaaa aacattagcc gtcacgctgg
     5281 caaaagcagg ttggcgagtg gctctcatcg aacaatcaaa tgcaatgtat ggcgggacct
     5341 gtattaatat cggctgcatc ccaaccaaaa cattggttca tgacgcacag cagcacacag
     5401 attttgtccg tgccatacag cgtaaaaatg aagtggttaa ttttttacgt aataagaatt
     5461 ttcataatct tgcggatatg cccaatatcg acgtgatcga cggccaggcg gagtttatca
     5521 ataatcatag cctgcgtgtt catcggcctg agggaaatct ggaaattcat ggcgagaaaa
     5581 tttttattaa taccggtgca caaaccgtgg ttccgccaat tcctggaatt accaccacgc
     5641 ctggtgtata tgacagcacc ggattactta atctaaaaga attgcctggg catttaggta
     5701 ttttgggcgg cggatatatt ggcgttgagt tcgcctctat gttcgctaat tttggcagca
     5761 aggtaaccat tttagaagca gcttcgctgt ttttgcctcg ggaagatcgg gatattgctg
     5821 ataatatcgc gacgatttta cgcgatcagg gcgtcgatat tatcctcaat gcccatgtgg
     5881 agcgaatcag tcaccatgaa aatcaagtgc aagtgcatag cgagcacgcc caactggcgg
     5941 tggatgcact gttaatagct tccggtcgtc aaccggctac cgcttcgtta catccagaaa
     6001 atgctggtat cgcagtaaac gagcgcgggg caattgtcgt tgacaagcaa ttacatacca
     6061 ccgcagacaa tatttgggcg atgggagatg ttaccggcgg gctgcaattt acttatattt
     6121 cactggatga ttaccgcatt gtgcgtgatg agttgctggg agaaggcaga cgtagtactg
     6181 atgatcggaa aaatgtgcct tattccgtat tcatgacacc gccactgtcc agggttggta
     6241 tgacagaaga acaggccaga gagagtggtg ctgatattca ggtggtgaca ttgcctgtag
     6301 ctgcaattcc gcgtgccaga gtgatgaatg atactcgtgg ggtattaaaa gcgattgttg
     6361 ataataaaac ccaacgtata ttaggggcat cactgctgtg tgttgactcc cacgagatga
     6421 tcaatatagt gaaaatggtg atggatgccg ggctgcctta tagcatatta cgcgatcaga
     6481 tatttactca tccgtcgatg agcgaatcac tcaatgatct attttcatta gtcaaataaa
     6541 ctcaaaatca gacgccagaa caaatattct ggcgtctcag agaaaagaat cttattaatt
     6601 ccctgttact ctata
//
LOCUS       DAZGVQ010000233         6360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000233 DAZGVQ010000000
VERSION     DAZGVQ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.235"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(89..1270)
                     /gene="cynX"
                     /locus_tag="ACHLAG_004872"
     CDS             complement(89..1270)
                     /gene="cynX"
                     /locus_tag="ACHLAG_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFP0657957.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPARVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPVRFPQLWVKE
                     A"
     gene            complement(1276..1746)
                     /gene="cynS"
                     /locus_tag="ACHLAG_004873"
     CDS             complement(1276..1746)
                     /gene="cynS"
                     /locus_tag="ACHLAG_004873"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFP0657958.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(1777..2436)
                     /gene="cynT"
                     /locus_tag="ACHLAG_004874"
     CDS             complement(1777..2436)
                     /gene="cynT"
                     /locus_tag="ACHLAG_004874"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFP0657959.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            2546..3445
                     /gene="cynR"
                     /locus_tag="ACHLAG_004875"
     CDS             2546..3445
                     /gene="cynR"
                     /locus_tag="ACHLAG_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFP0657960.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAKHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALDKCAVVGGNESR"
     gene            complement(3782..5065)
                     /gene="codA"
                     /locus_tag="ACHLAG_004876"
     CDS             complement(3782..5065)
                     /gene="codA"
                     /locus_tag="ACHLAG_004876"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFP0657961.1"
                     /translation="MSNNALQTIINARLPGEEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(5055..6314)
                     /gene="codB"
                     /locus_tag="ACHLAG_004877"
     CDS             complement(5055..6314)
                     /gene="codB"
                     /locus_tag="ACHLAG_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFP0657962.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEANSVE"
BASE COUNT         1566 a         1797 c         1689 g         1308 t
ORIGIN      
        1 ctgatacgct gcgcttatca ggcctacaag ttcagcgatc tacattagcc gcatccggca
       61 tgaacaaagc gcaggaacaa gcgtcgcatc atgcctcttt gacccacagc tgcggaaaac
      121 gtactggtgc aaaacgcagg gttatgatca tcagcccaac gacgcacagc gcatgaaatg
      181 cccagtccat caggtaattg ccgctgatac tacgcagcac gccagaaaac cacggggcaa
      241 gcccggcgat gataaaaccg attccctgca taaacgccac cagcttgcca gcaatagccg
      301 gttgcacaga gtgatcgagc gccagcagca aacagagcgg aaacgcgccg cccagaccta
      361 acccacacac catcgcccac aataccggca attgcagcgg caaccagata aagccgcaga
      421 accccaccag ttgtaacacc agcgccagca ttaacagttt gcgccgatcc tgatggcgag
      481 ccatagcagg catcagcaaa gctcctgcgg cttgcccaag cgtcatcaat gccagtaagg
      541 aaccgctgta ctgcgcgctg gcaccaatct caatatagaa agcgggtaac caggcaatca
      601 ggctggcgta accgccgtta atcagaccga agtaaacacc cagcgtccac gcgcggggag
      661 tgaataccac gcgagccgga gtggttgttg tcttgtggga agaggcgacc tcgcgggcgc
      721 tttgccacca ccaggcaaag agcgcaacaa cggcaggcag cgcccaccag gcgagtgttt
      781 gataccaggt ttcgctatgt tgaactaacc agggcgttat ggcggcacca agtccaccgc
      841 cgcccatcag agccgcagac cacagcccca tcaccagtgg cgtgcgttgc tgaaaccgcc
      901 gtttaatcac tgaaggcatc accgcctgaa tgataccgat ccccacccca ccaagcagtg
      961 cgctgctaag cagcagcaca ctttgggggt aaagctcacg catcaatgca ccgacggcaa
     1021 tcagcaacag actgatggcg acgctgcgac gttcgctgac atgctgatga agccagcttc
     1081 cggccagcgc cagcccgccc atggtgacca ccggcagagc ggtcaacagg gcagccacgc
     1141 taaagctcat tccgctcgcc tggcgcaatt gcggtagcag tggaccgacg gaggtgagca
     1201 gtggtcgcat attaagacca atcagcacca gaaccagcag catcgtgaac gtcttttgat
     1261 ggttgcgcat ggctgtcaga acggtttggt cggcagatat ttaccatcta aggtgatgac
     1321 cgcacgttcg ccaccttccg ggtccgccac tttcttaacg tcgagtttga agttaatcgc
     1381 gctgataatg ccatcgccaa acttctcatg aaccaacgct ttcagggttg tgccgtacac
     1441 ctgcaacatt tcatagaaac gatacatcgt tgggtcggtt ggaatacggt catcaatgca
     1501 gccacgcagt ggaatcatct gcaacagtag aatggcgtct tcgtcgagat ccagcttcgc
     1561 cccgaccagg cgggcggcgt cggcaggaag cgcctgctgc cccagcaaag ccgcggttac
     1621 gaaggcttct gccagaccgg taccgtcggc aatctcggca aatgagagat cttttttagc
     1681 tttgctgagc aaaatggcat cggcaagatc aagacgaata ttgcggttaa tttgtgactg
     1741 aatcatggtg gatctcctga tggtttaaaa ataaggttac gctgcggtcg gttggcgtag
     1801 cggtatggca caaacgcgag gattagcggc cagtggcaca aactggcggg ttgcgccgtc
     1861 aaaagctgcg atgctgccgc tttcaatgtc gtagacccag ccgtgcaggg cgatccgccc
     1921 ctcttcgagc gccaggcgca ccgatggatg agtttgcaaa ttagccaact gagcaatgac
     1981 gttttcacgt accatcgccg cagcttttga cggtaaatcg gaatgcgggc gcgcctcatt
     2041 aacgacgcgg gctgaatcgg cataacgcag ccagtgggag acggcaggca tatggtccat
     2101 acactgacag ctggcaatgg cggtcatcgc gccacagttg gaatgaccac aaatcacaat
     2161 gtcagatacc cgaagcgcag cgacggcata ctccaccgaa gcagaaacgc caccgggttc
     2221 cggcccgtag gaagggacaa tattacctgc gttgcgaata acgaacagat cgccaggctc
     2281 acgttgcgtc accagctcag ggaccagacg gctgtcggag caggagataa aaagggtgcg
     2341 cgggctttgc tgtgtcgcca gctgtttaaa taaggcttcc cgcttcggaa atgcctcgcg
     2401 ctggaattta aggaatccat caataatctc tttcaccgtt aacctctgtc tgtctctgga
     2461 atgagaggca gactactcgc cgattgtcat aaggtaaaag tctcatttat gatgagttct
     2521 attggattta cttataggtt ggcgaatgct ctctcgacat atcaactatt ttcttgccgt
     2581 ggctgaacat ggcagcttta cccgtgccgc cagtgcgttg cacgtctccc agcctgcgct
     2641 ttcccagcag attcgccagt tagaggagag tttaggcgtg ccgctgtttg accgtagcgg
     2701 gcgaacgatt cgtctcactg atgcaggaga agtctggcga cagtacgcca gccgggcgtt
     2761 acaggaactg ggagcgggta aacgggcgat tcatgatgtt gccgatctga cgcgaggatc
     2821 gctgcgtatc gccgtcaccc ccacctttac aagctacttt atcggccctt taatggcgga
     2881 tttctatgcg cgctaccccg gcatcacgct ccagctacag gaaatgtcgc aggagaaaat
     2941 cgaagatctg ctttgtcgcg acgagctgga cgttgggatt gccttcgcgc ctgtgcattc
     3001 gccggagctg gaggcaattc ctttactgac agaaagttta gcgttggtgg tggcgaaaca
     3061 tcatccgctg gctgcctgtg aacaggtggc gttgagtcgc ttgcatgatg aaaaactggt
     3121 cctgctcagc gcggaatttg ccaccagaga gcaaattgac cactactgcg agaaagcggg
     3181 gctacatcca caggtggtca ttgaggcgaa ctcaattagc gcggttctgg agctgattcg
     3241 ccgcacttcc ctttccacat tgttaccagc agcgattgcc acacaacatg acgggcttaa
     3301 agcgatttct cttgccccgc cactactgga gagaacggcg gttttgttgc ggcggaaaaa
     3361 tagctggcag acagccgccg cgaaggcatt tttgcacatg gcgttggata aatgcgcggt
     3421 tgttggcgga aatgaatcgc ggtagccgct gacacaggtc tgcggctgga attgcctgat
     3481 gcgctacgct tatcaggcct acgagttcag tgctctacgt aggccggata aggcgttcac
     3541 gccgcatccg gcataaacta cagctggcag actgccgctg cgaaggcatt tttgcacatg
     3601 gcgttggagg aatgcgcgga tgttggcgga aatgaatcac ggtagccgct gacacagatc
     3661 agcggctgga attgcctgat gcgctacgct tatcaggcct acgagttcag tgctctacgt
     3721 aggccggata aggcgttcac gccgcatccg gcataaacta cgctcgctgt aacccagccg
     3781 attaccgttt gtaatcgatg gcttctggct gctccagata caccgttgtt tgtgctggtt
     3841 gtgtactggc aatcaccttg ccgccacgta ccgaataacg taccggaacc tgacggcgca
     3901 gcgcatcaaa cccattttca gccggcagga taatcaggtt ggcgctgttt ccggcggcaa
     3961 tgccgtaatc ctgcaaattc aacgtccttg cgctgtggtg ggtgattaaa ttcaggccat
     4021 cgttaatctg cccgtagccc atcaactggc aaacatgcag ccccatatgc agcacttgca
     4081 gcatattcgc cgttcccagc ggataccacg gatcgaagac atcatcgtga ccaaagcaga
     4141 cgttaatgcc ggactccagc atctctttaa cgcgcgtgat gccgcgacgt tttggatacg
     4201 tatcgaaacg tccttgcaga tgaatattga ccagcgggtt ggcgacaaag ttaataccgg
     4261 acattttcag caagcggaac aggcgtgagg tatacgcccc gttataggag tgcattgccg
     4321 tggtgtggct ggcggtgact cgcgcgccca tgccttcacg gtgcgccagg gcagcaacgg
     4381 tttcgacaaa gcgcgactgc tcgtcatcga tctcatcaca gtgaacgtcg atgagacggt
     4441 cgtatttttg cgccagggcg aaggttttat gcagcgactc cacgccgtat tcacgggtaa
     4501 attcaaaatg cggaatcgcc cccactacat ctgcccctaa gcgtaacgcc tcttccagca
     4561 acgcttcacc gttgggatac gacaaaatcc cttcctgcgg gaaggcgacg atttgcagat
     4621 caatccacgg cgcgacttcc tgcttcactt ccagcattgc tttcagcgca gtcagcgttg
     4681 catccgaaac atcgacatgg gtacgcacat gctgaatgcc gttggcaatc tgccatttca
     4741 gcgtttgcca ggcgcgttgt ttcacatcgt catgggttaa taacgctttg cgttcggccc
     4801 agcgttcaat gccttcaaac agcgtgccgg actgattcca gttcggttgt ccggcggttt
     4861 gcgtggtgtc caggtgaata tgcggctcca caaacggcgg tataactaaa ccttgttcgg
     4921 catccaggct gttttcagtt atgggcatca cgccggattg cgcatcaatg gcgctgattt
     4981 ttccgtcctg cagatgaatc tgccacagcc cctcttcgcc tggtaaccgg gcgttaataa
     5041 ttgtttgtaa agcgttattc gacactgtta gcctccacat gcgtcattgc tgctgtcgtt
     5101 ttacgattca aaatcgggtt aaggatcaga tagctcagcg cgccacctaa taccgcgttg
     5161 accggaacaa ttcccggtaa ccagtggcct gcagcaatcc ccaaggcgac cgccagaatc
     5221 gccacccaat tgacactcat catacgcgtg gtcgcaaagt gctcatagcg gcgacggttc
     5281 atcagatagt cggcgatgat cacgccaccc actggaggaa tagctgccga aaggaaggtc
     5341 aaccagccga caaaattgtt atacagccat aatgcgcaga ccgtaccgat aataccgttg
     5401 attaccgaaa gggttttgct cgacatcccg gtaatgttgg cgaaacctaa acccgacgca
     5461 tagagtgcgt tatcgttggt ggtccagata ttcagcccca gcaccacaat cgcaggcagc
     5521 agcaggccct gagcaatcat cacatcagag atatccgcca tgcccagtgc cgcagcgccc
     5581 gctgcaccga aaataaacat caacgagttg ccgaggaaaa aggccaccat cgccaccagc
     5641 accgccagtt tggcattgcg accaaaccgg acaaagtcag cggtgagcgt acccgcactg
     5701 ataaatgacc ccacaaccag cgccagcgcg acattgaaat ctaacggttg tgcgggaacg
     5761 accgctttta atgcgtccag gccgcccatg ccgttaacag ccagccacac ggaataaccg
     5821 cccaggcagg cgatagccgg aaccgcaatc accgaaagaa ccgtcagcgc cgaaatgcca
     5881 aaaaagacgg tgacggtcat cagtaaaccg gaaacggcaa tcagcaaatt aatatccagc
     5941 ccggttgcct tacccaccgg aatggcaaac atcgccacac cgacgccaaa ccagccaacc
     6001 tgagttccgc ccagtagcag tgaaggcagc catgagcctt taacaccaaa cgagaagcga
     6061 gcaagaagat gagtggtcag gccggttttt gcgccaatgt aaccgagaaa tgaagtgtaa
     6121 ataccgagga gaagattacc gatgagaact gcgaggaaga aatcatgata gctaagaccg
     6181 gttccgagag tgccgccggt ccacatactg gcggaaaaga aggttaatcc cagcatgacg
     6241 aacgtcaatg ccaatacccc tttccgcgcc gactgcggga ctggcccctg gctaaagttg
     6301 ttatcttgcg acacgaaatt cccccttttt gctgtttgaa acccaaaaaa tccgccgcat
//
LOCUS       DAZGVQ010000234         6298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000234 DAZGVQ010000000
VERSION     DAZGVQ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.236"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(234..1904)
                     /gene="betA"
                     /locus_tag="ACHLAG_004878"
     CDS             complement(234..1904)
                     /gene="betA"
                     /locus_tag="ACHLAG_004878"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFP0657963.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1918..3390)
                     /gene="betB"
                     /locus_tag="ACHLAG_004879"
     CDS             complement(1918..3390)
                     /gene="betB"
                     /locus_tag="ACHLAG_004879"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFP0657964.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQDAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGNVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3404..3991)
                     /gene="betI"
                     /locus_tag="ACHLAG_004880"
     CDS             complement(3404..3991)
                     /gene="betI"
                     /locus_tag="ACHLAG_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFP0657965.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4120..6153
                     /gene="betT"
                     /locus_tag="ACHLAG_004881"
     CDS             4120..6153
                     /gene="betT"
                     /locus_tag="ACHLAG_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFP0657966.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTSTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1281 a         1731 c         1742 g         1544 t
ORIGIN      
        1 ctgtttgatt tgttcacgca taatatatcc agaaaataaa atctgtcgca gataaggttg
       61 tattaatagt ctgtatcagg aatgttcggg ttaaatatca gcaaaaagcc agcatcatga
      121 atactggata tgatgcatga gagttacctc agtgtttata taaggattcg gtccccctct
      181 ctggaacggt aactctcaat ctgatcggtt cctgcgttag ttcacatcac gactcatttt
      241 ttcgctctca ccggcatccc atttgccaca aaatatcccg ccgtgctcct cggcagcgct
      301 tcctgtccac gaatcatatc cgctattttc tcgccaatca taattgtcgt ggcgttcaaa
      361 ttcccggtga taatctgcgg cataatcgac gcatccacca cacgcaggcc ttctaacccg
      421 tgtacgcggc cttcgccgtc aaccacggac atctcgtcgt aacccatttt gcaggtaccg
      481 cacggatgga aggcggtttc ggcgtggtta cgcacgaact catcgagctg ttcatccgtc
      541 tgacattccg tgccggggct gatttcgcgg ccacgatact gatccagcgc gggttgatgc
      601 atgatctcgc gggtgatgcg aattgcgtcg cggaactcct gccagtcctg ctcgtgcgac
      661 atgtagttaa acagaatcgc cggatgctgg tgcgggtcgc gggatttaat ccgcacatgc
      721 ccacggcttg gcgagcgcat tgagccgacg tggcactgga aaccgtgctc tttcactgca
      781 ttcgagccgt tatagttaat cgctactggc aggaaatggt actgaatatt cggccacgca
      841 aattcctcac ggctgcgaat aaatccacct gcttcaaagt ggttgctggc accaacgcca
      901 gtgccgccaa acagccactc cgcaccgatt ttcggttggt tccaccactg cagggcaggg
      961 tagagggaaa ccggttcttt gcactcatat tgcagataca tctccagatg atcctgaaga
     1021 ttttcgccga cgccgggtaa ttcatgcacc agcggaatat caaactccgc cagcagttca
     1081 gcgttgccga cgccggagcg ttgcaggatc tgcggtgagg caatcgcgcc tgcacataac
     1141 agcacttctt tgttggccgt tgcgcgggtt gggatggtgc tgtcgccttc cagccattcg
     1201 acgcccaccg cgcgtttgcc gtcaaaaatg atgtgatcgg tcatagcgtg agtacgaatg
     1261 gtcaggttag ggcgcgattt ggcctgatcg agataaccgc gcgcggtact ggcgcgacgg
     1321 ccctgcggcg tgacggtgcg atccatcggg ccaaaacctt cctgctgata gccgttgaga
     1381 tcgtccgtgc gcgggtagcc tgcctgcacg cccgcttcaa tcatcgcttc aaacagcgga
     1441 ttgacgccgg gtttggaggt ggtgacgctc accgggccat cgccaccgtg gtagtcgttc
     1501 tcgcccacat cgcgagtctc ggccttgcgg tagtagggca ggcagtcgag ataactccag
     1561 ttctccagac cgggttcttg cgcccagtta tcgagatcca gcgcattgcc acggatgtag
     1621 cacatgccgt tgatcagcga cgatccaccc aggcctttac cgcgtccgca ctccatgcgg
     1681 cggttattca taaacggttc aggctctgtt tcataggccc agttgtagcg tttgccctgt
     1741 agtgggaacg ccagcgctgc gggcatctgg gtgcggaagt caaagcgata gtccgggccg
     1801 cccgcttcaa gcagcagcac ggaggtattc ggatcttcag tcagacgggt agcgagaacg
     1861 ttgccggctg agccggcacc aataatgatg tagtcaaatt gcaaataaac ctcctggtta
     1921 gaatatggac tggaatttag ccatctcaac ctggatggac ttcacctggg tgtaactctg
     1981 gagcgtcatc acgccgttct cgcgaccaat gccggagtgt ttgtagccgc caacgggcat
     2041 ctctgccggg gattcgcccc aggtgttgat ccagcaaata cccgcttcca gctgatgaat
     2101 gacgcgatgc gcgcggttca ggtccgccgt cacgatgccc gccgccaggc cgtagtcggt
     2161 atcattggcg cggcgaatca cttcgtcctc agattcataa gtgagaagcg acatcaccgg
     2221 accgaagatc tcttcgcgca cgatggtcat ttcgtcgcgg caatcggtga acacggtcgg
     2281 tgcaacccat gcgccgttat cgaagccatc gcctttcagt acattgccgc cgcacagtac
     2341 gcgcgcgcct tcctctttgc ctttggcgat atagcgcagc acgttatcgc gatgcgggaa
     2401 gctgaccagc gggccgaagt tagtttgcgg atcgaaaacg tcgcccgcgc gaatgcgctc
     2461 aacgcgcgcc agaattttct gctcaaatgc ggctttgcat ttcgccggaa cgaagacgcg
     2521 ggtgccgttg gtacacacct gaccggagct gaagaagttc gccatcatgg cgatatcggc
     2581 ggcgagatcg agatccgcat cgtcgaaaac gatcagcggt gatttaccgc ccagttccat
     2641 ggtcacttct ttcagggaag aggccgccga gttagccatc acttttttgc cgctggcgac
     2701 accgccggta aatgacactt tggcaatgcc cggatgctcg gtcagatatt gcccggtctc
     2761 cgcgcccacc cccggcaaca cgttaaatac gccgtccggc aggcccgctt cgctgtaaat
     2821 ttcagccagc tttaacgcgg taagcggggt gacttcgctc ggtttgaaaa tcattgcgtt
     2881 gcctgccgcc agcgccgggg cggatttcca cagggcaatc tggatcgggt agttccatgc
     2941 gccaatcccc gccaccacac ccagcggttc gcggcgggta taaacaaatg acgtctcacg
     3001 caacgggatc tggctgcctt ccagcgccgg gatcagcccg gcgtagtact ccagcacgtc
     3061 cgcaccggta acgatatcga cggttgaggt ttccgaatat gcttttccgg tgtcgagggt
     3121 ttcaagtttt gcgagttcgt cattgcgttc acgcagaata tcgacggccc gacgcagaat
     3181 gcgcgagcgc tccatcgcgg tcatcgccgc ccagattttt tgcccctgtt gggcactctt
     3241 cactgcgcga tcgacatcct cgcgcccggc gtcctgcacg gtcgccagca cgttaccgtt
     3301 ggccgggtta atggtctcga aggtgcgacc gctggtggcg gaggtataac caccatgtat
     3361 ataaagctgc tgttctgcca ttcgggacat cacgtctcct cggttaatcg gtgggtaaat
     3421 gctgagtaat aaagtgacgg gtcagcgagt gtgcgagagg tttatccagc ggtttgccgc
     3481 tcagtgccgc gcgcagccat aatccgtcaa tcagcgcggc aaggccgtag cccgcttcct
     3541 gtgcctgttc gcgcggtaac tcgcggcgaa attcgctcac cagattcgac aataagcggc
     3601 ggctactgac ctgctgtaaa cgatagagca tcggctgatg catactgctg gcccagaacg
     3661 ccagccaggc tttcatcgcc gcactgctca cctgcgtttc atcgaagttt ccgccaacaa
     3721 tcgcctgtaa tcgctgctct gcactgccct gcggaagtgc atgtaatcga ttcaaaaccg
     3781 cgtcacgcag ctgactggtg atatcgcgca tggttgcttc cagcagacca tttttgtccc
     3841 tgaaatagtg gctgatgatc cccgtagaaa cgcctgcacg gcgggcgatc tgcgcgatcg
     3901 ttgcatcgtg catgcccact tcatttattg cttccagtgt ggcgtcgatc agttgtctgc
     3961 gccggatcga ctgcatcccc aatttgggca ttttcgccac tccattcatc agcgttgttt
     4021 atctattaaa gcggttattg attggacgtt caatataaaa tgtgtcttaa ttgttacgaa
     4081 tttgatttta aatggtaaca ataacagtgg ggatactgga tgacagacct ttcacacagc
     4141 agggaaaagg acaaaatcaa tccggtggtg ttttacacct ccgccggact gattttgttg
     4201 ttttccctga caacgatcct gtttcgcgac ttctcggccc tgtggattgg ccgcacgctg
     4261 gactgggttt ctaaaacctt cggttggtac tatctactgg cggcaacgct ctatattgtc
     4321 tttgtggtct gtatcgcttg ttcgcgtttt ggttcggtga agctcgggcc agaacaatcc
     4381 aaaccggaat tcagcctgct gagttgggcg gcgatgctgt ttgctgccgg gatcggtatc
     4441 gacctgatgt tcttctccgt agccgaaccg gtaacgcagt atatgcagcc gccggaaggc
     4501 gcgggacaga cgattgaggc cgcgcgtcag gcgatggtct ggacgctgtt tcactacggc
     4561 ttaaccggct ggtcgatgta tgcgctgatg ggcatggcgc tcggatactt tagctatcgt
     4621 tataatttgc cgctcaccat ccgctcggcg ctgtacccga tcttcggtaa acggattaac
     4681 gggccgatag ggcactcagt ggatattgca gcggtgatcg gcaccatctt cggtattgcc
     4741 actacgctcg gtatcggtgt ggtgcagctt aactatggct tgagcgtact gtttgatatt
     4801 cccgattcga tggcggcaaa agcggcactg atcgccttgt cggtgataat cgccacgatc
     4861 tctgtcacct ccggtgtcga taaggggatt cgtgtgttat cggaacttaa cgttgcgctg
     4921 gcgctgggat tgatcctgtt cgtattgttt atgggcgaca cctcgttcct gcttaatgcg
     4981 ctggtgctga atgttggcga ctatgtgaat cgctttatgg gcatgacgct caacagtttt
     5041 gccttcgacc gtccagttga gtggatgaat aactggacgc tcttcttctg ggcatggtgg
     5101 gtggcatggt cgccgtttgt cggcttgttc ctggcgcgta tctcgcgtgg gcgtaccatt
     5161 cgccagttcg tgctgggcac gttgattatt ccgtttacct ccacgctgtt atggctctcg
     5221 gtgttcggca atagcgcgct gtatgaaatc atccacggcg gcgcggcatt tgccgaggaa
     5281 gcgatggtcc atccggagcg cggcttctac agcctgctgg cgcagtatcc ggcgtttacc
     5341 tttagcgcct ccgtcgccac cattactggc ctgctgtttt atgtgacctc ggcggactcc
     5401 ggtgcgctgg tgctggggaa tttcacctcg cagcttaaag atatcaacag cgacgccccc
     5461 ggctggctgc gcgtcttctg gtcggtggcg attggcctgc tgacgctcgg catgctgatg
     5521 accaacggga tatccgcgct gcaaaacacc acggtgatta tggggctgcc gttcagcttt
     5581 gtgatcttct tcgtgatggc ggggttgtat aaatctctga aggtagaaga ttaccgccgt
     5641 gaaagtgcca accgcgatac cgcgccgcga ccgctggggc ttcaggatcg cctgagctgg
     5701 aaaaaacgtc tctcgcgcct gatgaattat ccgggcacgc gttacactaa acagatgatg
     5761 gagacggtct gttacccggc aatggaagaa gtggcgcagg agctgcggtt gcgcggcgcg
     5821 tacgtggagc taaaaagcct gccgccggaa gagggacaac agttggggca tctggatttg
     5881 ttggtgcata tgggcgaaga acaaaacttt gtctatcaga tttggccgca gcaatattcg
     5941 gtgccgggct ttacctaccg cgcacgtagc ggtaaatcga cctactaccg gctggaaacc
     6001 ttcctgttag aaggcagcca gggcaacgac ctgatggact acagcaaaga gcaggtgatc
     6061 accgatattc ttgaccagta cgagcggcac cttaacttta ttcatctcca tcgtgaagcg
     6121 ccgggccata gcgtgatgtt cccggacgcg tgattgtttt tgcataaaac cgcgccatgt
     6181 ctgcatatgg cgcgggaatc attgactcgc tgtaaaaaat tatgcatata ttatatatga
     6241 ttgtcatgtc atatcctgaa attcctcttg tatcacttaa tgactttata ttttatta
//
LOCUS       DAZGVQ010000235         6271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000235 DAZGVQ010000000
VERSION     DAZGVQ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.237"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(37..1173)
                     /locus_tag="ACHLAG_004882"
     CDS             complement(37..1173)
                     /locus_tag="ACHLAG_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP0657967.1"
                     /translation="MDLLIVLTYVALAWTVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLAPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1176..1538)
                     /locus_tag="ACHLAG_004883"
     CDS             complement(1176..1538)
                     /locus_tag="ACHLAG_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP0657968.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2075..3988
                     /gene="mtlA"
                     /locus_tag="ACHLAG_004884"
     CDS             2075..3988
                     /gene="mtlA"
                     /locus_tag="ACHLAG_004884"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFP0657969.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4218..5366
                     /gene="mtlD"
                     /locus_tag="ACHLAG_004885"
     CDS             4218..5366
                     /gene="mtlD"
                     /locus_tag="ACHLAG_004885"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP0657970.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5366..5953
                     /gene="mtlR"
                     /locus_tag="ACHLAG_004886"
     CDS             5366..5953
                     /gene="mtlR"
                     /locus_tag="ACHLAG_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFP0657971.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(5965..6174)
                     /gene="yibT"
                     /locus_tag="ACHLAG_004887"
     CDS             complement(5965..6174)
                     /gene="yibT"
                     /locus_tag="ACHLAG_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFP0657972.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
BASE COUNT         1527 a         1608 c         1641 g         1495 t
ORIGIN      
        1 acaaggagca aagttccata atctcccccc tccccctcaa tgatccaaat aaagataatg
       61 catccagctg gtcattctta gcagcacttt ccgcatcacc gaaacatggc tgaaatggtc
      121 ggagtaaacc gccacctggg cgtagatgcc gtcgggtaag gcatcgatat catcgttagg
      181 gtccagttca atggttccca gcacaccgtc cgtgccgggc acgaccgtta atgattgcaa
      241 taccccctgc gcctgataag aaccgcctgg cacgacaggt aaaatactag tcagtttgcc
      301 gtgaaacacc tgcccaggta gcgcgttaaa caccacttcc gcatcatcgc cgggtttcag
      361 acgtaacagc gagttttgcc gaaattgggc gacaatttgc cgtttttgct cggggatgaa
      421 gaccatcacc ggacgcagcg gcaaggcagc tgcgtatgta cctgggcgga tcagtacctg
      481 agtaacgtag ccattgctcg gcgcgcgaat gacagtctgc tcaaggttat attttgcttc
      541 agtaagttgc gctcttaagc tcacaatctg cgattgctcg ccgttaacca tactgtcgag
      601 ctggctctgg atctgcgcct gctccgccac cgagcctttc accagcgcat cctgcgcgag
      661 gaaattttgc cgcgcatcgt cgatgtcacg ttccgagaac ggattcaccg ccgcctggct
      721 gcctttcaag taacgttgat aatttttaaa cagacggtcg cgctccgctg aaacctgggt
      781 ggtgttggcc tgcgcttcag tgagctgcgc acgcagcgtc tttatattat gcgtcgccgt
      841 catcaggtca gcctgaagtc tgtcaactcg cgcctggtaa cgaaccgggg cgagcttaaa
      901 aagcacctcg cccttttgaa taagctgatt attcttgtca gtgacttcag taacaattcc
      961 cgtcacctgt ggcgtgatag ggatcgctat cactgccttt tgcgcggtaa aagtgtaagg
     1021 gtggttgtag ttcatcaaca aaattaaacc actcaccaga aacacgcctc ccagcgccgc
     1081 cgtcgccagc gtccactgat ttaccggaat gcggaagatt ttaaagaccg tccacgccag
     1141 cgccacgtaa gttaaaacaa tcaatagatc cataattaga tctccgggaa cgtggaatag
     1201 tcagtctttt tctcggcggc gagttgatgc tccagccggg aaattcggtc agaaagtgag
     1261 gcgatttccg ggtcagtcgc tttctcctgc gacgcaacat gtgactgcat accccagcca
     1321 cgctccggtt gatagagcgt cgcccagatc cacagaaacg gccagataac atgcagagta
     1381 aacaggctca cccagcccgc cgtatgaata gcgtcggcat gggggtgatt gcgctttttg
     1441 gcaatcagat aagggatgtc atgtatcgcg atgattccat aaaaaatcac cagaaagacg
     1501 aagatcagca ctcccaacgc gaaatagttt agaaacatat ctgcctcgga ttcacgttta
     1561 tcagtgttgt ttttgggctg gcagccagaa gggagtcagg ctgatatttt gacaataatc
     1621 cgggttcgcg attctcgcca taacaccaaa gaataatttt tagaggtgat gagttgctta
     1681 gttacataac gattgtatga cgaaggcata acatgctgta gatcacatca ggtgaacgcc
     1741 gtaagaaaat atcttgtgat tcagatcaca aagattcaac aaaccatcaa aacaaaaatg
     1801 tgacactact cacatttaaa tgccattttt agcgaaaatc gccgtcttgt tgctttttta
     1861 cacaagcgtt ttgtgatgag tatcacgtca attacctctc taccccctat atttatgtga
     1921 ctgatatcac acaaaaggcc gtcgactgga cagttaaccg attcagtgcc agatttcgca
     1981 gtatctacaa ggtccggcta cctctgccgc cacattaaca aaaaacctcg ggcttccagc
     2041 ctgcgcgaca gcaaacataa gaaggggtgt ttttatgtca tccgatatta agatcaaagt
     2101 gcaaagcttt ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg cgtttatcgc
     2161 gtggggtatc atcaccgcgt tatttattcc aacagggtgg ttaccgaacg agacgctggc
     2221 gaagctggtc gggccgatga tcacttatct cctgccgctg ctgatcggtt ataccggtgg
     2281 taagctggta ggcggcgaac gtggcggcgt agtcggtgcc atcactacca tgggcgttat
     2341 cgtcggcgca gacatgccga tgttcctcgg ttctatgatt gcaggtccgc tgggcggctg
     2401 gtgcattaag cacttcgacc gctgggtaga cggtaagatc aaatccggtt ttgagatgct
     2461 ggtgaataac ttctccgcag gcatcatcgg gatgatcctc gccattctgg catttctcgg
     2521 cattggcccg attgttgaag ccctgtccaa aatgctggct gcgggcgtta acttcatggt
     2581 tgtccatgac atgctgccgc tggcgtctat ctttgttgaa ccggcgaaaa tcctgttcct
     2641 caacaacgcc attaaccacg gtatcttctc gccgctgggt attcagcagt cccatgaact
     2701 gggtaaatcc atcttcttcc tgattgaagc taacccaggt ccaggtatgg gcgtgctgct
     2761 ggcgtacatg ttctttggtc gtggtagtgc taaacagtct gcgggcggtg cggcaatcat
     2821 ccacttcctg ggtggtatcc acgaaatcta cttcccgtat gtgctgatga atccgcgtct
     2881 gatcctggcc gttatcctcg gcggtatgac tggcgtgttc acgctgacta tcctgggcgg
     2941 tggtctggtt tctccagcat ctccggggtc tatccttgcg gtactggcga tgacgccaaa
     3001 aggtgcttac ttcgctaaca tcgcgggtgt gtgtgcggcg atggctgtct ccttcgttgt
     3061 ctctgctatt ttgctgaaaa ccagcaaagt gaaagaagaa gatgatattg aagcagcaac
     3121 tcgtcgtatg caggacatga aagctgagtc taaaggcgca tctccgctgt ctgctggcga
     3181 tgtgactaac gacctgagcc acgtacgtaa aatcatcgtt gcctgtgacg ccggtatggg
     3241 ttccagtgcg atgggcgcag gcgttctgcg taagaaaatt caggatgcag gtctgtcgca
     3301 gatttctgtt actaacagcg cgatcaacaa cctgccgcca gatgtggacc tcgtcatcac
     3361 tcaccgtgac ctgaccgaac gcgctatgcg ccaggttccg caggcacagc atatttcgct
     3421 gaccaacttc ctcgacagcg gcctgtacac cagcctgacc gaacgtctgg ttgctgccca
     3481 acgccacacg gcaaacgaag agaaagtaaa agacagcctg aaagacagct ttgacgattc
     3541 cagtgctaac ctgttcaagc taggcgcgga gaacatcttc ctcggtcgca aagcggcaac
     3601 caaagaagaa gcgattcgtt ttgctggcga gcagctggtg aaaggcggtt acgttgagcc
     3661 ggaatacgtt caggcgatgc tggatcgtga aaaactgacc ccgacttatc tgggtgagtc
     3721 tatcgcggtg ccacacggta cggttgaagc gaaagatcgc gtactgaaaa cgggcgtggt
     3781 gttctgccag tacccggaag gcgtgcgctt cggtgaagaa gaagatgaca ttgcccgtct
     3841 ggtgattggt attgcagccc gtaacaacga gcacattcag gttatcacca gcctgaccaa
     3901 tgcactggat gatgagtccg tcatcgagcg tctggcacac accaccagcg tggatgaagt
     3961 gctggaactg ttggcaggtc gtaagtaatc caatcccacc ctctccacat ggagaaggtg
     4021 gggttaattg cctgatgcgc tacgcttatc aggcctacag gatgcatcac gatttgttga
     4081 atttgcacgt tcttgtaggc cggataaggc gcttacgccg catccggcgc tgcccctctc
     4141 ctcacggaga gggtttgggt gagggaaaag cctcacccca gccctctcgg gtaaaaacat
     4201 tgatgaaggt taatactatg aaagcattac attttggcgc aggtaatatc ggtcgtggct
     4261 ttatcggtaa actgctggca gacgcgggta tccaactgac gtttgccgat gtcaatcagg
     4321 tggtacttga tgccctgaat gcccgtcata gctatcaggt tcatgtggtt ggtgaaaccg
     4381 agcaggtaga taccgtttcc ggcgtcaatg ctgtcagcag cattggtgat gatgtcgttg
     4441 atctgattgc tcaggtcgat ttagtcacta ccgccgttgg cccggttgtg ctggaacgta
     4501 ttgctccggc tatcgccaaa gggctggtga aacgcaaaga acaaggtaat gaatccccgt
     4561 tgaacatcat cgcctgtgaa aacatggtac gcggtaccac gcagctgaaa ggccatgtga
     4621 tgaacgccct gccggaagac gccaaagcgt gggtagaaga acacgttggc tttgtcgatt
     4681 ccgccgttga ccgcatcgta ccgccttcgg cttcggcaac taacgatccg ctggaagtga
     4741 cggtagaaac tttcagcgaa tggattgtcg ataaaacgca gttcaaaggc gcactgccga
     4801 acatcccagg catggagtta accgacaacc tgatggcatt tgtcgaacgt aaactcttca
     4861 ccctgaacac gggtcatgct ataaccgcgt acctcggaaa actggccggt catcagacca
     4921 ttcgtgacgc tattctcgac gagaaaatcc gcgcggtggt aaaaggtgcg atggaagaaa
     4981 gtggtgcggt actgatcaag cgctacggct ttgacgcaga caaacatgcg gcgtacatcc
     5041 agaaaatcct cggtcgtttt gagaacccgt atctgaaaga tgatgtagag cgcgtaggcc
     5101 gtcagccgct gcgtaaactg agtgctggcg accgtctgat caagccattg ctcggtacgc
     5161 tggaatacag ccttccgcac aagaatctga ttcaggggat tgctggtgca atgcacttcc
     5221 gcagtgaaga tgatccgcag gctcaggaac tggcagcact gatcgctgac aaaggtccgc
     5281 aggcggcgct ggcacagatt tccggtcttg atgccaacag cgaggttgta tccgaggcgg
     5341 taaccgctta taaagcaatg caataatggt ggaccaggcg caggacaccc tgcgcccgaa
     5401 taacagattg tcagatatgc aggcaacaat ggaacaaacc caggcctttg aaaaccgtgt
     5461 gcttgagcgt ctgaatgctg gcaaaaccgt gcgaagcttt ctgatcaccg ccgtcgagct
     5521 tttgaccgag gcggtaaatc ttctggtgct tcaggtattc cgcaaagacg attacgcggt
     5581 gaagtatgct gtagaaccgt tactcgacgg cgacggtccg ctgggcgatc tttctgtgcg
     5641 tttaaaactc atttacgggt tgggcgtcat taaccgccag gaatacgaag atgcggagct
     5701 gctgatggca ttgcgtgaag aactaaatca cgacggcaac gagtacgcct ttaccgacga
     5761 cgagatcctt ggaccctttg gtgaactgca ctgcgtggcg gcgttaccac cgccgccaca
     5821 gtttgaacca gcagactcca gtttgtatgc aatgcaaatt cagcgctatc aacaggctgt
     5881 gcgatcaaca atggtccttt cactgactga gctgatttcc agaatcagct taaaaaaagc
     5941 ctttcaaaag taagcaacgt ctgcttactg cccctctacc tgcttcggcc gataaagccg
     6001 acgataatac tccagacgtt gtagatataa cggcacgctt tcatcgggta ttccggacgg
     6061 aatcgcgtta cggggaggaa gttttttcag atactcccgg aacgcctggc ttgatgccat
     6121 gaaatctacg gctttatcga taagaagcgg aacgttttca cctaatttgc ccatgatgat
     6181 atctccgtat taccccgcgc cgggaatgcg cggccgccaa ttttagttta ggttcccggt
     6241 aaagatgaca ttaaggaaaa cgtgctgaat c
//
LOCUS       DAZGVQ010000236         6253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000236 DAZGVQ010000000
VERSION     DAZGVQ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.238"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            104..1036
                     /locus_tag="ACHLAG_004888"
     CDS             104..1036
                     /locus_tag="ACHLAG_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP0657973.1"
                     /translation="MNSVFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(1078..2166)
                     /locus_tag="ACHLAG_004889"
     CDS             complement(1078..2166)
                     /locus_tag="ACHLAG_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP0657974.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(2308..6049)
                     /locus_tag="ACHLAG_004890"
                     /pseudo
     CDS             complement(2308..6049)
                     /locus_tag="ACHLAG_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         1531 a         1662 c         1351 g         1709 t
ORIGIN      
        1 gacgggtatt aaaagttatt caaaaatgat tatctatctt taatcgatac ctctacagtc
       61 atggctggag aaattcatat tagataatgg cgggaatggt ttaatgaact cagtttttac
      121 cgaagagaat ctgttggcat ttactaccgc tgcgcgtttt ggcagtttca gtaaagccgc
      181 agaagagttg ggtttaacca cttccgccat tagctacacc atcaagcgaa tggaaacagg
      241 gctggatgtg gtgctgttca ctcgcagtac ccgcagcatt gagttaacgg agtccgggcg
      301 ttattttttc cgcaaagcta ccgacctgct gaatgatttt tatgccatca aacgtagcat
      361 agatactatt tcgcagggca tagaagcgcg tgtgcgcatt tgtattaatc agctgcttta
      421 tacgcccaaa cacaccgcgc gcttattaca ggtgctgaaa aagcagtttc ctacctgcca
      481 gattaccgtc accaccgaag tgtataacgg cgtctgggat gcgattatta ataatcaggc
      541 caatatcgcc atcggcgcac ccgatacact actggacggc ggcggtattg attacactga
      601 aattggcgcg attcgctggg cttttgctat cgccccggat cacccgttag cctttgtgcc
      661 ggaacccatc gccgaaagcc aactgcgtct ctaccctaat attatggtgg aggacaccgc
      721 gcatacgatt aataaaaaag tgggctggtt gctgcacggg caggagtcaa ttctggtgcc
      781 agatttcaac accaaatgtc agtgccagat tttaggtgaa ggaattggtt ttttgccgga
      841 ttacatggtc cgtgaggcga tgacgcaatc cctgctggtt acccgacaaa tccataatcc
      901 gcgccaggat tcgcgcatgt tactggcgac tcagcattct gctacgggtc aggtgacgca
      961 gtggattaaa aaacaatttg cgccaaatgg catattaacg gggatctatc aggatttact
     1021 gcatcgggag aattaatcgc tgccaacctc ccgatgcgct gtgaatagcg gtttacatca
     1081 accacctgct ttcattaccc attcagagat aaatttatta cccggtactg gcggagagta
     1141 caaatagccc tgcaaaaagt gaacgccttt accgatcatt aaatccgcct gctcctgggt
     1201 ttctacccct tccgccacga tactcagacc aggcttacgc gctagttcga caatattgtc
     1261 cacaatatga ccggagattt cgtcgacact cgccatttgc acaaatgact tatcgatctt
     1321 aataaaatcg accgggaacg cctgcaagta acgataggtc gcataacccg taccaaagtc
     1381 atccagcgca aaggtaatgt tgtgctgatg aaggctgtca aatatcgctc tggcttctgg
     1441 cgttaccgga atagggttac gttccgttag ctcgagaacc agcttgattt tatcgttacc
     1501 taatttctta accaggttca gacactcttt ttcaaatccc gctgccaaaa aacaacccgc
     1561 cgagacgttg atgccaatat ggaaattgtc cggcagcaaa tgttttaccg gcatcagaat
     1621 atccgcagtc tgtttcatca gttggcgggt cattatgaca ataagaccgg atgactccgc
     1681 cagaggaata aactgatccg gtgggataat tcccgtttgt ggatgttccc agcggacaag
     1741 tacctcacag cccgtcagta ccccagtctg cgcgcagaaa accggttgga tccacggttt
     1801 gaattcgtgg ttttccaacg ccagactgat ggcttcaggc gtgacgatat ggtgacaacg
     1861 gttatcaggg atataaaaat tatttttatt tcccgtgcca ctgatgactt gcggatgata
     1921 ttgaaaaaag atatcccgcc agaaggtaat atcactctgg atgcctagtt tttcatacag
     1981 ctgcttcttt tgatgtgaaa ttgtctttgc actgcgattt ctgtactgtg agatttctgt
     2041 aaccgacatt ccctccatta gcagcttgag taaaagccgt tctttttcgg acacgatacc
     2101 aggcaacgtc aaattcaccg ttgccccgaa ctcttccaaa aaaacactca aatcatgcga
     2161 agccatcgaa cacaccttga ttaaatcttg tgcgccagct aaatctgcga gtcttcactc
     2221 tgactgataa agctggctgc aagaaaatga cacagagagc cgaattatct cccgttcggc
     2281 tctctctcct tttactcatt attcagatca gaattgccac ttaatgccaa ccattgcaga
     2341 ggtgtcatta tatcccctat cggcaacctg aacgcccaca ttcccccaga cattcaggtt
     2401 ggcatttagc tgtccttcca ccccggtttt tatctcagca atatttcggg ctccatcctg
     2461 agtgacggac acgccatcca tactggtgct gaaatcctta ctgttatgca gccagttcac
     2521 ttctacaaac ggctggaact cgcgggattt accgtcatcc attttatggt ggctcttcag
     2581 ccaggttttt acgccaagtc gggtttgaac attgccatca ccgttgctat gaacgagggt
     2641 tccgttgctt tcgcggtgct tatcggcttt gactcccatc caggtaacct gtgcttgcgg
     2701 ctgaacatac cattcattac gcgttccctg gctgccatta aattcagcta atttgtgttt
     2761 gtatccagct tccagtgaag cggtaaatcc ttttgattta taggattcac tttgtaagtc
     2821 atcccctttc actgtgttat caaaccagct gtactgcgcc caactgtcga gatacgcgcc
     2881 attacgcgac tcgtcatcgg cataccaggt ggcatagagg ccggtgctat atccgttcac
     2941 actcgctttt gcacgataac cggttcgcga ggaaatggtt ttactgtcgc tgttgccata
     3001 tcccgccatg accccaacat gccagctgtc gctgccgttt tggctccact gcgcgacatc
     3061 gcctcccagt tgcagaacat agcgattgct ttgggttttc agctggccgc tgccatcacg
     3121 ccatttatta tgaccacctt catggcgcat ccacatagtg gtttgtttct gctcacccgt
     3181 caccatgtcg gtatagtacg tattacccag acgctcatgc agacgcgtgg tgaacattgt
     3241 attcgctgct gcaaggttcg caatgtagct acccgcctcc ggtcgcaggt cattatccac
     3301 attcagatcc ggacccggcg taggtgtcgg gttagggttc ggctctggat tcgggtttgg
     3361 ctccggattc ggcatcgggt ctggctccgg ctgcagttca ggagaatcac tgccgctggt
     3421 cagataccag ttaccactat ttgccccttg tccacgcgcg agagtgtagt catacgcccc
     3481 cgcaacgata cgaccagcct gaacaaattc gccctcagac ttaccgtcta catggataag
     3541 ttcgataccg ttaagggttt tcgcacctgt accgccagcg ttattcaccg taacggcagt
     3601 cgtgccgcta gtatcgccct cgacaaccaa tttatcggtt actgagtcat cgccattcag
     3661 taccgtattc attactagtt gaccgttatt accgtggtag ttccccttca ccactaacac
     3721 attgccagct tgctgtcctg acattccggt catcagcgta ccgttgttgg tcagattgcc
     3781 gcccagggtg taattcacag acgcagccgc tcttttttca gcgctattat tcaacgccag
     3841 ggtaccgttg ttctcaacgt ttgcgctacc caatccctgc gcatgactta ccaggacatt
     3901 agccgaatta gcaatgttca cattcgctgt caggttgctg ttatcgcccg cgatgtccag
     3961 cgtgccgccc tgaatattca gctctccgct ccccgttaac gaaccggttg aagtgccccc
     4021 gttggtaacc gtcagactgc cgccatttaa gctcagcagt gaatcggcgc tgccattgag
     4081 cgtgcccacg gtctggctgt gaccattcat atccagttca gtctctgccg ccagacgcag
     4141 ttcatgggta ttacccaaca cgttatcgtt attcaacaaa agcgtcccac tgcgcagatc
     4201 ggtaacaccg gtatagtcgt tgtctttgtt agaaagcgat acggtctgac ccttctggct
     4261 gctgaatgcc aggtcaccac tgccggtaat ctttgcgctg agatcggcgg cattctcgct
     4321 tttaccgtta gagctcagca ccagagcatc gctgtcggta gcgtgtaaat caagctgggt
     4381 caggccgtaa ccgatataca gaccgtcatt gtttacccca ctcgtcagac gatagtcgta
     4441 agtgccgacg gccgcttttt gcccattctg ctgaatatca cgttgggcac tgctggagag
     4501 gatttgccca tttttatcct gcaattgcag attgcccgca tcgcccagaa cttccgcacc
     4561 ttgcgcatca accaacttaa tggtgctgtt accatcatcg acttcagtga gcgataacgc
     4621 agagtcaata tcacgagata ctgagcttac aacgtcactg tcagaaacct gaatcacacc
     4681 ctcgccgcgc aaatccagcg ttttactaac gttgaccgtc ccttctgtca tctgtgcacc
     4741 tgctgtcagg gcaccaaaat caacggttcc gccggagaat cccaaagtgc taatggagtg
     4801 agcgccctta cccactgtta ccaggcttcc ctgacccgca gtgatcccgc ctctctgcag
     4861 aaactcagca ttcttttcca ggttaaatgt cgtgttctgg aacgccagtg aaccctggaa
     4921 ccagtctgta taagcaaact catcgaagga gaattcgttt attggcaaag gtcatgctat
     4981 cgtcagcctt caggtgtaat ttcccgcggt tgttgatctc tgctgtacct atatttttgg
     5041 tgatccctaa agcgccattt tgctcaattt tgactttacc actaaaggca gagttatcac
     5101 cggttaagta agtcgtgcca ttgagcgcat tgatatagcc caggccctcg ctgccactga
     5161 caatatccgc ggaaatagtc gcgtttttat ccgtgtggtt taaggtgatt ttcttatcgg
     5221 atgtctttgc ccacacaaac tcaatttttt cggcatcaat aatccctgca gccttcgcag
     5281 cacttccttc ctgtgcacct aacgctaact cagagttggt gcttaaacta attttcggtg
     5341 cggatatttt acctccatct gatacgacta atgttgtatc agaacgttgg tcgtaataac
     5401 ccaggctaat atgttcacga gcattaactt caccaccgtt attaaccgac aggaactgat
     5461 taccgccata tccgccgtaa acgcccataa agaaggtccc tgcatttacc tgactacctt
     5521 cgccgcttac attcccctgc gtataagtca gacgactggt gcccttaaca tgaattttgc
     5581 cgccattgga gacattaaaa tagccttgat catgtctcat gcctgagttt gactcataca
     5641 aatcatcagc atataactcg ctgccagcat tggagacatt caggaacccc gtacctttca
     5701 gcaaaaagtt aagcgggcca ttgattgtta cctttcctcc ggtaatatcc aacccaccac
     5761 cagaagcgag agtcagacgc ggacgggaag tatctgtcgt attgattacc agattggcat
     5821 tgtcagtcac cgtaatacgg taatcgatgc tctggttctc tttgccaaaa tcaatagctt
     5881 tatcctggtt tacgatgaca tcatcggcga ataccagaga tgaatataaa cttatcgcgc
     5941 taattaaaac agcccgggac ttttttcctt ttactctgct ccccagctcc gagcagacgc
     6001 tccaacagtt acgggacctg ttccacttta gccgatatat tttattcatc acaatctcca
     6061 gcaatacatc caaataaaac caagattgca taaacagcaa cgcattaaat gccattctgg
     6121 ttactgttca tcgcatataa aaattcaaaa attttgcata gcattggcaa ccacatatct
     6181 gagtatgtag ttcagagggt aataaaatag caagttacta ctgaaaactt attttaagtt
     6241 aacactctta act
//
LOCUS       DAZGVQ010000237         6176 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000237 DAZGVQ010000000
VERSION     DAZGVQ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.239"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            79..855
                     /gene="exuR"
                     /locus_tag="ACHLAG_004891"
     CDS             79..855
                     /gene="exuR"
                     /locus_tag="ACHLAG_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFP0657975.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1095..1178
                     /gene="yqiM"
                     /locus_tag="ACHLAG_004892"
     CDS             1095..1178
                     /gene="yqiM"
                     /locus_tag="ACHLAG_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFP0657976.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1200..1862
                     /gene="yqjA"
                     /locus_tag="ACHLAG_004893"
     CDS             1200..1862
                     /gene="yqjA"
                     /locus_tag="ACHLAG_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFP0657977.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1866..2249
                     /gene="mzrA"
                     /locus_tag="ACHLAG_004894"
     CDS             1866..2249
                     /gene="mzrA"
                     /locus_tag="ACHLAG_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFP0657978.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2396..2764
                     /gene="yqjC"
                     /locus_tag="ACHLAG_004895"
     CDS             2396..2764
                     /gene="yqjC"
                     /locus_tag="ACHLAG_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFP0657979.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2802..3107
                     /gene="yqjD"
                     /locus_tag="ACHLAG_004896"
     CDS             2802..3107
                     /gene="yqjD"
                     /locus_tag="ACHLAG_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFP0657980.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3110..3514
                     /gene="yqjE"
                     /locus_tag="ACHLAG_004897"
     CDS             3110..3514
                     /gene="yqjE"
                     /locus_tag="ACHLAG_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP0657981.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3504..3803
                     /gene="yqjK"
                     /locus_tag="ACHLAG_004898"
     CDS             3504..3803
                     /gene="yqjK"
                     /locus_tag="ACHLAG_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFP0657982.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3989..4381
                     /gene="yqjF"
                     /locus_tag="ACHLAG_004899"
     CDS             3989..4381
                     /gene="yqjF"
                     /locus_tag="ACHLAG_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP0657983.1"
                     /translation="MKKLEGVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4451..5437
                     /gene="yqjG"
                     /locus_tag="ACHLAG_004900"
     CDS             4451..5437
                     /gene="yqjG"
                     /locus_tag="ACHLAG_004900"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFP0657984.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5730..6095
                     /gene="yhaH"
                     /locus_tag="ACHLAG_004901"
     CDS             5730..6095
                     /gene="yhaH"
                     /locus_tag="ACHLAG_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP0657985.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1546 a         1520 c         1551 g         1559 t
ORIGIN      
        1 atttatatat gaccaatggc atacccatga ataaagtggt atgataactt cgtccgcatt
       61 ccactccggg agacgcatat ggaaatcact gaaccacgcc gtttgtatca acaacttgcc
      121 gctgacctga aagagcgcat cgaacagggc gtctatctgg taggtgataa actgcctgca
      181 gaacgcttta ttgccgatga aaagaacgtc agccgtacgg tagttcgtga agccatcatc
      241 atgctggaag ttgaaggcta tgtcgaagtg cgtaaaggtt caggtattca tgtggtttcc
      301 aaccagccgc gccatcagca ggcggctgac aataatatgg aattcgccaa ttacggtccg
      361 tttgagttgc ttcaggctcg ccagctcatc gaaagtaata ttgccgagtt cgcggcaact
      421 caggtaacga aacaagacat catgaaactg atggccattc aggaacaggc gcgcggcgaa
      481 caatgcttcc gtgattccga gtgggattta cagttccaca ttcaggtagc cctggcgacg
      541 cagaactccg ccctggcggc catcgttgaa aaaatgtgga cccaacgtag ccataacccg
      601 tactggaaaa aactgcacga acacattgat tcccgtaccg tcgataactg gtgtgatgac
      661 cacgatcaaa tcctcaaggc gctgattcgc aaagatcctc atgctgctaa gctggcaatg
      721 tggcagcacc tggaaaacac caagatcatg ttattcaacg aaaccagcga cgacttcgag
      781 ttcaatgccg accgctatct gttcgccgaa aacccggtag tgcatctcga tacggccacc
      841 agcggcagta aatgaaatac ctgtccgaca ggcgctgcga cgcgcctgtt tgtctcgtcg
      901 gtaagcgaaa agtataaagt gtcagcctgt gtaaatcctc tcgccaccct cccctgcatt
      961 cagcaaaatc agactccacg gacatgtaat tttgataacg aacaacgttg acctttgtta
     1021 caattagatt caatttgaat ttatgttttt gaatgctttc ttatctcacg atttaacagg
     1081 gaatagttca ggctgtgttg atgtatcaaa cccgcagaac ataccaaaac agcaataaca
     1141 ttgcggtagt gcatctttta aaaccagcgt ggcgttaacc gattcaccag gaataatgaa
     1201 tggaactttt gacccaattg ctgcaagccc tgtgggcgca ggattttgaa accctggcca
     1261 atccatcgat gattggcatg ttgtattttg tcttgtttgt aattttgttc cttgaaaacg
     1321 gcttgcttcc agcggccttt ttaccgggcg acagtttact ggtattggtc ggcgtgttga
     1381 ttgcgaaagg cgcgatgggt tacccgcaaa cgattctgct gctgaccgtt gccgccagcc
     1441 tcggctgctg ggtcagctat attcaggggc gatggctggg caatacccgc accgtacaaa
     1501 actggctatc tcatttaccc gcgcattatc atcaacgcgc acaccatctt tttcataaac
     1561 acggtttatc ggcgctgtta attggtcgct ttattgcgtt tgtcagaaca ctgctgccga
     1621 cgattgccgg gttatcaggg ctgaataacg cgcgctttca gtttttcaac tggatgagcg
     1681 gtctgctgtg ggtattgatc ctgacaactc tgggttacat gctcggcaaa acgccggtat
     1741 ttttaaagta cgaggaccag ctgatgtcat gcctgatgct gctcccggtg gtgctgctgg
     1801 tgtttggcct ggcaggttct ctggtcgtgt tatggaaaaa gaaatatgga aatcgggggt
     1861 aagggatgca aatacctcgc atgtcgcttc gccagctagc ctggtccggc actgttttac
     1921 ttctggtcgg cacgctgttg ctggcctggt ccgcggttcg ccagcaagag tctacgctgg
     1981 cgattcgtgc cgttcatcaa ggcacaacga tgccagacgg tttttcgatc tggcatcacc
     2041 ttgacgctca tggcattcct ttcaaaagta tcacccccaa aaacgacact ctgttaatta
     2101 catttgactc cagcgaccag agcgccgccg caaaagcggt cctcgacaga acattgcccc
     2161 atggctacat cattgcgcag caggacaata acagtcaggc tatgcagtgg ctgacccggt
     2221 tacgggataa ctctcatcgc ttcggataac ttcctgttat ccgaaacata tcactcactt
     2281 tggtgatttc accgtaactg tctatgatta atgaggcggt ggttaacact accgcttcgt
     2341 tgtctggaca atcgttcctt tgtaataggt ccgaatcaca atggaaggtt caagaatgaa
     2401 ataccgcatc gctttagctg tttctctctt tgctcttagt gccggtagtt atgccactac
     2461 cctgtgtcag gaaaaggagc aaaatatcct taaggagatc agctatgccg aaaaacacca
     2521 aaaccagaat cgtattgacg gtctgaataa agccctgagt gaagtccggg ccaactgttc
     2581 agatagccag ctgcgtgccg atcatcacaa gaaaatcgca aagcagaaag atgaggtggc
     2641 ggaacgccag caagatttag ccgaggcgaa gcaaaaaggc gatgccgata agattgccaa
     2701 acgcgaacgg aaactggcag aagcgcagga agagctgaaa aagctggaag cgcgcgacta
     2761 ctaactcaca atagtcacta cttactcacc tggagaaaac tatgtcgaaa gaacacacta
     2821 cggaacatct gcgtgctgag ttgaaatccc tttccgatac gctggaagag gtgcttagct
     2881 catctggcga gaagtcgaaa gaagagttga gtaagattcg tagcaaagcg gagcaggcac
     2941 tgaaacagag ccgttatcgc ctgggtgaaa ccggtgatgc catcgccaaa caaacccgtg
     3001 tcgcggcggc gcgtgctgat gagtatgtgc gtgaaaatcc gtggaccggc gtgggcattg
     3061 gcgctgcaat cggtgtagtg ctcggcgttc tgctgtcgcg tcgttaatta tggcggacac
     3121 tcatcacgcg caagggcccg gtaaaagcgt tctgggcatc gggcagcgaa ttgtttccat
     3181 catggttgaa atggtggaaa cacgtctgcg gctggcggtg gtggagctgg aagaggaaaa
     3241 agctaatctc tttcaacttt tactgatgct gggcctgacg atgcttttcg ctgcatttgg
     3301 tcttatgagc ctgatggtgc taattatttg ggcggttgat ccgcaatatc gcctgaatgc
     3361 gatgattgcc accaccgtgg tgttgctgct actggcactg attggcggta tctggacgtt
     3421 acgtaaatcg cgtaaatcca ccttgctgcg ccatacacgc catgagctgg caaacgatcg
     3481 gcaactgctc gaggaggagt cccgtgagca gtaaagtcga acgtgaacga cgtaaggcgc
     3541 aactgcttag ccagatccag caacaacggc tggatctttc cgccagtcgt cgtgaatggc
     3601 tggaggcaac aggcgcttac gatcgtcgct ggaatatgct gctaagtctg cgctcctggg
     3661 cgctggttgg cagtagcgtg atggcgatct ggacgattcg ccatcctaat atgctggtcc
     3721 gctgggccag acgcggtttt ggcgtatgga gcgcctggcg tctggttaaa acgaccctca
     3781 agcagcaaca gcttcgcggt taacttccct ctggccggag caattccggc cttatccctc
     3841 aaattttttg aagatttttg acagttttcc ttgctaacaa tcatcattca ccacgtttat
     3901 gattctctcc atcgacagca acgacgctaa taccgcgccc ttgcacaaaa aaacaatcag
     3961 cagcctgagt ggcgcagtgg agagtatgat gaaaaaatta gaaggtgttg gtgtactggt
     4021 agcgcgcatt ttaatgccga ttctgtttat taccgcaggc tggggaaaaa tcaccgctta
     4081 ctctggtacc caacaatata tggaagcaat gggtgtcccg ggttttatgc tgccgctggt
     4141 gattctgctt gagttcggtg gtggtctggc aatcctgttc ggtttcctga ctcgcaccac
     4201 agccctgttt actgcgggct ttacgctgct gacggcattt ttatttcaca gcaactttgc
     4261 tgaaggcgtc aactcgctga tgttcatgaa aaacctgaca atttctggcg gattcctgct
     4321 gctggcaatt accggtccgg gcgcgtatag catcgaccgc ctgctgaata aaaagtggta
     4381 agcacgcgct atacttaacg ataaaaaacg aggaggaagc tcctcgtttt tgctattgga
     4441 ggagagaaaa atgggtcaac tgattgacgg cgtctggcat gacacctggt acgataccaa
     4501 atctaccggc ggtaaatttc aacgttcagc ttccgcattt cgtaactggc tcactgccga
     4561 tggcgcacct ggccccactg gcacaggcgg ttttatcgca gagaaagatc gttatcatct
     4621 ctatatttca ctcgcctgcc cgtgggcgca ccgcacgctg atcatgcgca aactcaaagg
     4681 actggaaccg tttatttccg tttccgtagt gaacccgctg atgctggaaa acggctggac
     4741 ctttgatgac agttttccgg gagcaaccgg agatacgctc tatcaacatg aatttctgta
     4801 tcagctttat ctccacgccg atccacacta cagcggacga gttactgttc ccgtgctgtg
     4861 ggacaaaaag aaccacacca tcgtcagcaa cgaatcagca gaaatcatcc gcatgttcaa
     4921 taccgcgttt gatgcgctgg gcgcaaaagc gggtgattac tacccaccag ccctgcaaac
     4981 gaaaattgac gaacttaacg gctggattta tgacaccgtt aacaacggcg tgtataaagc
     5041 tggttttgcc accagccagc aagcctacga cgaggcggtg gcgaaagtgt ttgaatcgct
     5101 ggcgcgactg gagcagattt taggccagca tcgttacctg accggcaacc agctaaccga
     5161 agccgatatt cgcctgtgga ccacgctggt ccgttttgat ccagtgtatg tgacccactt
     5221 caagtgtgat aagcaccgca tcagcgatta ccttaatctg tatggcttcc tgcgtgatat
     5281 ctaccagatg ccgggaattg ccgaaacagt caatttcgat cacatccgta atcattactt
     5341 ccgcagccat aagaccatca atcctacggg gattatttca attggtccat ggcaggatct
     5401 cgatgaaccg catggacgag atgttcgctt cggttaaaaa aaggcacctt acgaggtgcc
     5461 tctgataatt agctaagtgc atgttgttca tgtcggatgc ggcgtgaacg ccttatcaaa
     5521 cctacaaaac atttatattc aatatgttgc aatgaccgcg caggccagat aagcgtagcg
     5581 catctggcga ttttgcgctt gtcatcagtc ttaacgcgcc ttcacagcac tttattaatt
     5641 ccttgaacga atatttactg ccatttcatt cacgtctatt cttaatttgc tgctttaaaa
     5701 acaagtgatt gagcaaaatt gaggcaaaaa tggactggta tctgaaagta ctaaaaaatt
     5761 atgtcggttt ccggggtcgt gcgcggcgca aagagtactg gatgtttatt ctggtcaaca
     5821 tcatctttac gttcgtgctg gggctgctgg ataaaatgtt aggctggcaa cgcgctggtg
     5881 gcgaaggcat cctgacgaca atctacggta ttctggtgtt tttaccgtgg tgggcagttc
     5941 agttccgccg cctgcacgac accgaccgct cggcgtggtg ggcactgttg ttcttaatcc
     6001 cgtttatcgg ctggcttatt atcatcgtct ttaactgcca ggcgggtacg ccaggcgaaa
     6061 accgctttgg accagatccg aaactggaac cttaaaacaa atctgctcgc cggatgtctg
     6121 actcatccgg cttttatcat catgttgatg aatgaataat ttactaatgt ttattt
//
LOCUS       DAZGVQ010000238         6116 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000238 DAZGVQ010000000
VERSION     DAZGVQ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.240"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            456..905
                     /gene="alaE"
                     /locus_tag="ACHLAG_004902"
     CDS             456..905
                     /gene="alaE"
                     /locus_tag="ACHLAG_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFP0657986.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(942..1286)
                     /locus_tag="ACHLAG_004903"
     CDS             complement(942..1286)
                     /locus_tag="ACHLAG_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFP0657987.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1438..1767
                     /locus_tag="ACHLAG_004904"
     CDS             1438..1767
                     /locus_tag="ACHLAG_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFP0657988.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            2015..2260
                     /gene="nrdH"
                     /locus_tag="ACHLAG_004905"
     CDS             2015..2260
                     /gene="nrdH"
                     /locus_tag="ACHLAG_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFP0657989.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2257..2667
                     /gene="nrdI"
                     /locus_tag="ACHLAG_004906"
     CDS             2257..2667
                     /gene="nrdI"
                     /locus_tag="ACHLAG_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP0657990.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2640..4784
                     /gene="nrdE"
                     /locus_tag="ACHLAG_004907"
     CDS             2640..4784
                     /gene="nrdE"
                     /locus_tag="ACHLAG_004907"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP0657991.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYTYTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFTRSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4794..5753
                     /gene="nrdF"
                     /locus_tag="ACHLAG_004908"
     CDS             4794..5753
                     /gene="nrdF"
                     /locus_tag="ACHLAG_004908"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP0657992.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
BASE COUNT         1571 a         1512 c         1470 g         1563 t
ORIGIN      
        1 tctgctcgct aattccgcaa gcctgacaaa accgaagtga ataaaaatgc aatatgacag
       61 aataaaagaa aacacccctc actggtgctt tctgctgtta atgtacattt tctggcataa
      121 aaaatgctgt aaatgacccg gtcacgacat acaccgaatt ctctacgctt atagcgttac
      181 ctcaccccca aactttcatt agtgaaatta aaggcaaggt catatctaat tgatttaatt
      241 gaaaataaat catatcggca tgtaaaaatc cacagtgtac aaaaaacgag ccgttacgga
      301 atattttatc tacaaaaact gactaaataa aaatttttca ctaattgaat agtcatagcc
      361 agcgatatac gctatgcgaa aatgcagatg gcaatgagat ccactgcttt catctccatt
      421 aacatcccat tacgctttta ttaaggagca ttagcatgtt ctcaccgcag tcacgcttgc
      481 gtcatgcagt tgcagatacg ttcgcgatgg ttgtttactg ttctgtcgtg aacatgtgta
      541 ttgaagtttt cctctccgga atgagcttcg aacagtcttt ttattccaga ttggtagcga
      601 ttccggtgaa catcttaatt gcatggccat acggtatgta ccgcgatctg tttatgcgcg
      661 cggcacgcaa agttagcccg tcgggctgga taaaaaatct ggctgatatc ctggcttatg
      721 tgacgttcca gtcaccggtg tatgtggcga tcttgttagt ggtgggcgca gactggcatc
      781 agattatggc ggcggtcagt tcaaacatcg ttgtttcgat gttgatgggg gcggtttatg
      841 gctacttcct cgattattgc cgccgactgt ttaaagtcag ccgttaccag caggtaaaag
      901 cctgactctt cttttcgcga ctggcatcgc cagtcgcgtc tttaactggc ttcgccaaat
      961 aaaccattca aataacgttc aagcgcaaca cgcgaactaa agccatgcgg gataccgtaa
     1021 tgctcgtgtc gctcgcctgc taaatagagc ggaaattgct ggtaatgatc gcaagtttta
     1081 atatcggaag cgggttcagc cagtgttgaa ctgcgttctt ttaatgttgg atgaattacc
     1141 agagcggtac gtcccattcg cgcttcgcga ttaacataga cataattttc cccacggcgg
     1201 taaccatacg ctttctgggt taccacatcg acagtaaaac cgactttttc aagtacgcgc
     1261 gccacctcgt ctggtcgtaa atacatattt gatcctcgtt attatcaacc gcgggcctac
     1321 cttacctgat tgcgcattca caacgctttc agaaaagtcc ataaagccgc gatgaacctc
     1381 agttaagaaa attatggtct acactgaaaa ttacatcgaa ttctaatggg ggatcatatg
     1441 tttaaccgac cgaaccgcaa cgacgttgat gatggcgtgc aggatatcca gaatgatgtc
     1501 aatcaattag ctgacagcct cgaatctgta ttgaagtcct ggggcagcga cgccaaaggg
     1561 gaagctgaag ccgcacgcag taaagctcag gcattgctga aagaaaccag ggcgcgaatg
     1621 catggtcgta ctcgcgtcca gcaagccgca cgcgatgccg ttggctgcgc ggattctttt
     1681 gttcgtgaaa gaccctggtg cagcgtgggt acagcagctg cggtaggtat ttttatcggc
     1741 gcactgttaa gcatgcgcaa atcgtagtgc aaaaatgata ataaatacgc gtctttgacc
     1801 ccgaagcctg gcttcggggt ttctttttgc ctggtgaatc acaaaaatcc ccctaccccg
     1861 tcacgctcat atccagggta atttcgacca ccatttgcta tatattgtgt ggttgaatct
     1921 tttttcaact acatctagta tctctgtatc aacagagaga caacccgacg cgtatcatcg
     1981 cgccgtatct tcattttaaa cggaaatacg aatcatgcgc attactattt acactcgtaa
     2041 cgattgcgtt cagtgccacg ccaccaaacg ggcgatggaa aaccggggct ttgattttga
     2101 aatgattaat gtcgatcgcg ttcctgaagc agcggaagcg ttgcgtgctc agggctttcg
     2161 tcagttgccg gtagtgattg ctggcgatct tagctggtct ggtttccgtc cggacatgat
     2221 taaccgtctg catccagcgc cacacgcggc cagtgcatga gccagctcgt ctacttctcc
     2281 agcagctccg aaaacacgca gcgttttatc gaacgtttag gtctgcccgc ggtgcgcatc
     2341 ccgctcaatg agcgggaacg gattcaggta gacgagcctt acatcctgat cgtgccctct
     2401 tacggcggcg gcggtacggc tggcgcggtg ccacgacagg taattcgctt tttaaacgac
     2461 gagcacaacc gggcgttgct tcgcggcgtt attgcttctg gtaatcgcaa ctttggtgag
     2521 gcgtatggcc gcgccggaga tgtgattgcc cggaaatgcg gcgtgccgtg gctgtaccgt
     2581 tttgaactca tgggtacgca aagcgatatc gaaaacgttc gtaaaggagt aaccgaattt
     2641 tggcaacgac aaccgcagaa tgcctgacgc aggaaacgat ggattaccac gcgctgaatg
     2701 cgatgcttaa cctctacgat agcgcaggtc gcattcagtt cgataaagac cgccaggccg
     2761 ttgacgcctt tattgcgacg catgtgcgtc cgaacagtgt gaccttcagt agccagcagc
     2821 agcgcctgaa ctggctggtc aacgaaggtt actatgatga aagcgttctt aatcgctact
     2881 ctcgcgactt tgtcattacg ctgtttgccc acgcacacac cagcggtttt cgtttccaga
     2941 cattcctcgg ggcatggaag ttttacacca gctatacgtt gaagacattc gacggtaaac
     3001 gttatctgga agattttgcc gatcgagtca cgatggtggc gctgacgctg gcacaaggcg
     3061 atgcgacgct ggcgttgcaa ctgaccgatg aaatgctgtc aggacgcttt cagccagcca
     3121 cgccaacatt cctcaactgc ggtaagcagc agcgcggcga actggtttcc tgttttctgc
     3181 tgcgtattga agacaatatg gagtcgattg gccgggcggt aaattccgca ctgcaactgt
     3241 cgaaacgcgg cggcggcgtg gcatttttgc tgtcgaatct gcgggaagcg ggcgcgccaa
     3301 ttaaacgtat tgaaaatcaa tcttctggcg taattccggt gatgaaaatg ctggaagacg
     3361 cattttccta tgccaaccaa ctcggcgcgc gtcagggggc tggtgcagtc tatttacatg
     3421 ctcatcatcc cgatattctg cgttttctcg acacgaaacg ggaaaatgcc gacgaaaaaa
     3481 tccgcattaa aacactgtcg cttggcgtgg tgatcccgga tatcactttc catctggcaa
     3541 aagagaatgc gcagatggcg ctgttttcgc cctatgacgt tgagcgagtt tatggcaagc
     3601 cgtttgccga tatcgccatt agcgaacact atgacgaact ggttgccgat gaacgcatcc
     3661 gcaaaaaata cctcaacgcc cgtgatttct tccagcgact ggcagaaatc cagtttgagt
     3721 ccggctatcc ctacatcatg tatgaagaca cggtaaaccg tgctaaccct atcgccgggc
     3781 gcataaatat gagtaatctc tgctcagaaa ttttgcaggt taacagcgcc tcagagtatg
     3841 acgagaatct cgactatacc tacacaggcc atgatatctc ctgcaattta ggttcgttga
     3901 atattgcgca caccatggat tcccccgatt ttgcccgcac ggtagagact gccgtgcgcg
     3961 gtttaacggc agtatcagat atgagtcata tccgcagcgt gccgtccatc gaagccggaa
     4021 atgccgcctc gcacgccatc ggactggggc agatgaattt acacggctat ctggcgcgag
     4081 aaggcatcgc ttatggttcg ccggaagcac tggatttcac caatctctat ttctatgcca
     4141 tcacctggca cgcactgcgt acctcgatgt tgctggcacg cgaacgcggt gaaaccttcg
     4201 ccgggttcaa acagtcacgc tatgccagtg gtgaatattt tagccaatat ctgcaaggga
     4261 actggcagcc gaaaacggcg aaagttggcg aactgtttac ccgtagcggt attacgttac
     4321 ctacccgtga gatgtgggcg cagctgcgcg acgacgtgat gcgctacggc atttacaacc
     4381 agaatcttca ggcggtgccg ccaaccggtt ctatctctta tatcaaccat gctacgtcga
     4441 gtattcatcc gattgtggcg aaagtagaga tacgcaaaga gggcaaaaca ggacgcgttt
     4501 actaccctgc cccgtttatg actaacgaaa atctggcgct atatcaggac gcttacgaaa
     4561 tcggcgcaga aaagatcatc gacacctacg cggaagcgac tcgccatgtc gatcaggggc
     4621 tgtcgctgac gctgtttttc cccgataccg ccaccactcg cgatatcaac aaagcgcaga
     4681 tttacgcctg gcgcaaaggt atcaaaacgc tctattacat ccgcctgcgt cagatggcgc
     4741 tggaaggcac tgaaattgaa ggctgcgtct cctgtgcact ttaaggaata tctatgaaac
     4801 tctcacgtat cagcgccatc aactggaaca agatatctga cgataaagat ctggaggtgt
     4861 ggaatcgcct gaccagcaat ttctggctac cagaaaaggt gccgctgtcg aacgatattc
     4921 ctgcctggca gacattaact gtcgtagaac aacaactgac gatgcgcgtt tttactggcc
     4981 tgacgctgct cgacacgctg caaaatgtta tcggcgcgcc ttcgctgatg cccgatgcac
     5041 tcacgcctca tgaagaagcg gtattatcga atatcagctt tatggaagcg gttcatgccc
     5101 gctcttacag ttcgattttc tcgacgctgt gtcagaccaa agatgtcgat gccgcctacg
     5161 cctggagtga agaaaacgca ccgttgcagc gaaaagctca gattattcag caacattatc
     5221 gcggtgatga tccgctgaaa aagaaaatcg ccagtgtgtt tcttgaatct tttttgttct
     5281 attccggttt ctggctgccg atgtattttt ccagccgcgg aaagctgacc aataccgcag
     5341 atctgatccg tctgattatc cgcgatgaag cagtccacgg ttactacata ggctataaat
     5401 atcagaaaaa catggaaaag atatctctgg gacaacgtga agagttgaag agtttcgcct
     5461 tcgatttgtt gctggaactc tacgacaacg agttgcaata caccgatgag ctgtacgccg
     5521 aaaccccgtg ggctgacgat gtgaaagcgt ttctctgtta caacgccaat aaggctttga
     5581 tgaatctggg ctacgaaccg ttatttcccg cagaaatggc ggaagtgaat ccggcaatcc
     5641 tcgccgcgct ttcgccgaat gccgatgaaa atcacgattt cttttccggt tcaggctcct
     5701 cttatgtgat ggggaaagcg gttgaaacag aagatgaaga ctggaatttc tgagggtgtt
     5761 attttcaaaa atatcactac ccgcagcagg gaaataattc ccgccaaata gctttttatc
     5821 acgcaaatat tctgtagtga tctacactga tactctgttg cattattcgc ctgaaaccac
     5881 aatattcagg cgttttttcg ctatctttga caaaaaatat caacttttct cgatttgctc
     5941 tcagccctta tatcacggga aattccggcg atttactcgc gtcaatattc atgccacatt
     6001 tgccatcagg ggttgcctca gattctcagt atgttagggt agaaaaaagt gactatttcc
     6061 attgggtaat atatcgacat aaacaaataa caggaatctt tctattgcat ggcaat
//
LOCUS       DAZGVQ010000239         6115 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000239 DAZGVQ010000000
VERSION     DAZGVQ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.241"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            75..1625
                     /locus_tag="ACHLAG_004909"
     CDS             75..1625
                     /locus_tag="ACHLAG_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MchC protein"
                     /protein_id="HFP0657993.1"
                     /translation="MSHQCSLSELNENLVPFTARQIKASLIWCAEDVRNPDELQNACS
                     YIIDPGSTASAKVFHAERYGGSGIQRNGGGARCGFDGNYQVKGIGSNPLVGEGTDERH
                     SNGALGAVHAIYEALWGEVLAQILPYSAVRVRAVLLTDLYTEKAFERSGRKSRRALLV
                     REPVVRPAHFERAPYFQVKPEYSSLLIHDACRVRSVIHKLPGYLPVPPEEIDAEARTD
                     PRIYCIEGLCELARREAWQMAFCRTRFLRLTTSPSNIAMDGRLMDFNGLSCLFPGDSP
                     ADFGYKLRLAELAKEPMVLMQGLSDLCLYIGKYMFDPDFTLAARLKVEEIFQKTFHEA
                     CYYCYLELLGIPGEFITQKEIPDILKELVNSFVALLNQYCEKSHAQDIVNQDGSPLQK
                     LVVTLIHHRHNQKQALNSSIKNDVYFTVAQQCFSQAIHWLTQGSTRRQINASLLLKEI
                     EHHTMKRLQPREELRKENMCEKIAILLDNHGDDPLFLQEAISDMKNFMLKFSRDAFGY
                     LEPIRNTV"
     gene            1651..2103
                     /locus_tag="ACHLAG_004910"
     CDS             1651..2103
                     /locus_tag="ACHLAG_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFP0657994.1"
                     /translation="MSYIRETIRGKDEWTVYEQIGFAVSCMLYNRNYSLYPVLTIQYW
                     TEYAIQHNQIKFLFDSRGFPVAYITWAYLEADTEARLLRDPEFRLHPSEWNEDGRIWI
                     LDFCCKPGFGRKVIDYLIQLQPWGEGEVRWLSRRKKIVTYIPERLHKT"
     gene            2255..3529
                     /locus_tag="ACHLAG_004911"
     CDS             2255..3529
                     /locus_tag="ACHLAG_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP0657995.1"
                     /translation="MFRQDALENRKMKWQGRAILLPGIPLWLIMLGSIVFITAFLMFI
                     IVGTYSRRVNVSGEVTTWPRAVNIYSGVQGFVVRQFVHEGQLIKKGDPVYLIDISKST
                     RSGIVTDNHRRDIENQLVRVDNIISRLEESKKITLDTLEKQRLQYTDAFRRSSDIIQR
                     AEEGIKIMKNNMENYRNYQAKGLINKDQLTNQVALYYQQQNNLLSLSGQNEQNALQIT
                     TLESQIQTQAADFDNRIYQMELQRYELQKELVNTDVEGEIIIRALTDGKVDSLSVTVG
                     QMVNTGDSLLQVIPENIENYYLILWVPNDAVPYISAGDKVNIRYEAFPAEKFGQFSAT
                     VKTISRTPASTQEMLTYKGAPQNTPGDSVPWYKVIAMPEKQIIRYDEKYLPLENGMKA
                     ESTLFLEKRRIYQWMLSPFYDMKHSATGPLND"
     gene            3522..5624
                     /gene="mchF"
                     /locus_tag="ACHLAG_004912"
     CDS             3522..5624
                     /gene="mchF"
                     /locus_tag="ACHLAG_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin export peptidase/ABC transporter MchF"
                     /protein_id="HFP0657996.1"
                     /translation="MTNGNFRQIINQLDMRWRRRVPVIHQTETAECGLACLAMICGHF
                     GKNIDLISLRRKFNLSARGANLAGINGIAEQLGMVTRALSLELDELGALKMPCILHWD
                     FSHFVVLVSVKRNRYVLHDPARGRRYLGREEMSRYFTGIALEVWPGSEFLAETQQTRI
                     SLRSLINSIYGIKRTLAKIFCLSVVIEAINLVMPVGTQLVMDHAIPAGDRGLLTLISA
                     GLMFFILLRAAVSMLRAWSSLVMSTLINIQWQSGLFNHLLRLPLAFFERRKLGDIQSR
                     FGSLDTLRATFTTCVVGAIMDSIMVVGVFVMMLLYGGYLTWIVLGFTMVYVLIRLVTY
                     GYYRQISEETLVRGARASSYFMESLYGIATVKIQGMAGIRGTHWLNLKIDAINSGIKL
                     TRMDLLFGGINTFVAACDQVAILWLGASLVIDNQMTIGMFVAFGSFRGQFSDRVASLT
                     SFLLQLRIMSLHNERIADIALHEKEEKKPEIEIVADMSPVSLETTDLSYRYDSQSAQV
                     FSGLNLSVAPGESVAITGASGAGKTTLMKVLCGLFEPDSGKVLVNGTDIRQLGINNYH
                     RMIACVMQDDRLFSGSIRENICGFAEETDDEWMTECAKASHIHDVIMKMPMGYETLIG
                     ELGEGLSGGQKQRIFIARALYRKPGILFMDEATSSLDTESERFVNAAIKKMNITRVII
                     AHRETTLRTVDRIFRITN"
     gene            complement(5685..6008)
                     /locus_tag="ACHLAG_004913"
     CDS             complement(5685..6008)
                     /locus_tag="ACHLAG_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0657997.1"
                     /translation="MRNITTDELALIHGAGLDKDASVAIGTAAGGFLGKATKIPGADI
                     AGSAIGGYIGGVIADGSNRTITIPSNGINYNPGIGLGSFNPNYNGGLLNSGFNSSTSS
                     SSSGS"
BASE COUNT         1765 a         1160 c         1460 g         1730 t
ORIGIN      
        1 agaagctgtc aggacactta cttgctcttt tatgaacact cttttatgaa aattgaaacg
       61 gagaatactg ccagatgagt catcagtgtt cactttctga actgaatgaa aacctggtgc
      121 ctttcactgc caggcagatc aaggcctcat taatctggtg tgcagaggat gtcagaaatc
      181 cagacgaact gcaaaatgcc tgcagttata ttatcgatcc tggcagtaca gcttctgcca
      241 aagtgttcca tgcagagcgc tatggtggca gtggtattca gcgtaatgga ggtggtgcac
      301 gttgtgggtt tgatggtaac taccaggtta aaggaatagg aagtaatccg ttggttggcg
      361 aaggtactga cgaacgtcat tctaatggtg cactcggcgc tgttcatgca atatatgagg
      421 ctttgtgggg agaagtactg gctcaaatat taccttatag tgctgtgcgg gttcgggcgg
      481 ttttacttac agatctctat actgaaaagg catttgagcg ctccggtagg aaatcacgaa
      541 gagccctgtt ggtacgtgag cctgttgttc gtccggcgca ttttgaacgg gcaccttact
      601 tccaagtaaa accggagtat tccagtctgt taattcacga tgcctgtcgg gttagatctg
      661 tgatccacaa gctgccagga tatctacctg taccaccgga agaaattgat gctgaagcac
      721 gaactgatcc ccggatttat tgcattgagg ggttatgtga actggcacgt cgtgaggcct
      781 ggcaaatggc attttgtcga acacgtttcc tgagattgac aacttctcct tctaatattg
      841 caatggatgg cagattaatg gattttaacg gactcagttg tctgtttccg ggagattccc
      901 cagctgattt tgggtataaa ctaagattag ctgaactggc aaaagaaccg atggtactta
      961 tgcaagggct gtctgatctc tgcttgtata tcggaaaata tatgtttgac cctgacttca
     1021 ctcttgcagc ccgtttgaag gttgaggaga tatttcagaa aacttttcat gaagcatgtt
     1081 attactgtta tctagaactg ttgggtattc ctggagaatt tataacacaa aaagagatac
     1141 ctgatatatt gaaagaactg gttaacagtt ttgttgcatt actcaatcaa tactgcgaga
     1201 aatcacatgc ccaagatatt gtcaatcagg atggttcacc attgcaaaag ttggttgtga
     1261 cgctaatcca tcataggcat aatcaaaagc aggcactgaa tagtagcatc aagaatgatg
     1321 tttatttcac cgttgcacaa cagtgttttt cccaggccat ccactggctg acgcaaggca
     1381 gtaccagacg tcagataaat gcctcattac tcctgaaaga aattgaacat cataccatga
     1441 aaaggctgca acccagggaa gagctgagga aagaaaatat gtgcgaaaaa attgccatcc
     1501 tgctggataa tcatggcgat gatccccttt ttttacaaga agcaatttct gatatgaaaa
     1561 attttatgct taagttttcc agagatgcat ttggatatct tgaaccgata agaaacacag
     1621 tgtaaaaaat atataaggga aatagtaatc atgtcttata taagggaaac catcagagga
     1681 aaagatgaat ggactgttta tgaacagatc ggttttgcgg tcagttgtat gctctacaat
     1741 cgtaattaca gtctgtatcc ggtgttaacc attcaatact ggactgaata tgcgatacag
     1801 cataatcaga ttaaattcct gtttgattca cgaggttttc cagtggcgta tattacctgg
     1861 gcatatcttg aggctgatac agaagcgcgc ctgctcaggg atccagaatt caggttgcat
     1921 ccgtctgaat ggaatgaaga tggaaggatt tggattctgg atttctgttg taaaccaggc
     1981 tttggtcgaa aagttattga ctatctcata cagcttcagc catggggaga aggagaagta
     2041 cgatggttaa gcaggcgaaa gaaaattgtg acatacatcc ctgagcggct gcataaaacg
     2101 tagtacctct gaagatacag agataattgt aaattacggg gtaaatgcat cgctgatact
     2161 attttgcagg actctgtatt ttctgtcttt gattatagca tttcgcttaa ccagtgaagg
     2221 tattacagga aaagtgtgag taagaggagc tattttgttt cgtcaggatg ctttagaaaa
     2281 cagaaaaatg aagtggcagg gacgggcaat attacttccc ggaataccac tatggttaat
     2341 catgctggga agcatagtgt ttattacggc atttctgatg ttcattattg ttggtaccta
     2401 tagccgccgt gttaatgtca gtggtgaggt cacaacctgg ccaagagctg tcaatatata
     2461 ttcaggtgta cagggatttg ttgtcaggca gtttgttcat gaaggacagt tgataaaaaa
     2521 aggggatcct gtttatctga ttgacatcag taaaagtaca cgtagtggta ttgtcactga
     2581 taatcatcgt cgggatatag aaaaccagct ggttcgtgtg gacaacatta tttcccgtct
     2641 ggaagaaagt aaaaaaataa cgctagatac cctggaaaaa caacgcctgc aatacacaga
     2701 tgcgtttcgt cgctcatcag atattataca gcgtgcagag gaagggataa aaataatgaa
     2761 aaacaatatg gagaattaca gaaactatca ggcaaaaggg ctgattaata aagatcagtt
     2821 aactaaccag gtggcattat attatcagca acaaaacaat cttctcagcc tgagcggaca
     2881 gaacgaacag aatgccctgc agataaccac tctggagagt cagattcaga ctcaggctgc
     2941 agattttgat aaccgtatct accagatgga actgcaacgg tacgagttac agaaagaact
     3001 ggttaacact gatgtggagg gcgaaatcat tatccgggcg ttgactgacg ggaaagttga
     3061 ctccctgagt gtcactgtcg ggcaaatggt caataccgga gacagccttc tgcaggttat
     3121 tcctgagaac attgaaaact attatcttat tctctgggtc ccgaatgatg ctgttcctta
     3181 tatttcggct ggtgacaaag tgaatattcg ttatgaagcc tttccggcag aaaaatttgg
     3241 gcagttctct gctacggtta aaactatatc caggactcct gcgtcaacac aggaaatgtt
     3301 gacctataag ggagcaccac agaatacgcc gggcgactct gttccctggt ataaagtcat
     3361 tgcgatgcct gaaaagcaga ttatcagata tgacgaaaaa tacctccctc tggaaaatgg
     3421 aatgaaagcc gaaagtacac tatttctgga aaaaaggcgt atttaccagt ggatgctttc
     3481 tcctttctat gacatgaaac acagtgcaac aggaccgctc aatgactaac gggaatttca
     3541 gacaaattat aaatcagctt gatatgcgct ggcgacgtcg tgttccggtt attcatcaga
     3601 cggagaccgc tgaatgtgga ctggcctgcc tggcaatgat atgcggtcat tttggtaaga
     3661 atattgacct gatatctctt cgccggaagt ttaatctctc ggcccgtgga gcaaaccttg
     3721 caggaatcaa tggaatagcg gagcagctgg ggatggtcac ccgggctctt tcactggagc
     3781 tggatgaact tggtgccctc aaaatgccgt gtattctcca ctgggatttc agtcacttcg
     3841 tcgtgctggt cagcgtaaag cgtaaccgtt atgtactgca tgatccggcc agaggcagaa
     3901 gatatctcgg tcgggaggaa atgagccggt attttacggg cattgcactt gaggtctggc
     3961 ccggaagtga attcctggcg gaaacccagc agacccgcat aagtctccgt tcactgatta
     4021 acagtattta cggtattaaa agaacactgg cgaaaatttt ctgtctgtca gttgtaattg
     4081 aagcaatcaa tctggtaatg ccggtgggaa ctcagctggt tatggatcat gcgattccgg
     4141 cgggggacag agggctgctg acgcttattt ctgctggcct gatgttcttt atattgctca
     4201 gggccgcggt gagtatgctg cgtgcatggt cctcactggt tatgagcacg ctcatcaata
     4261 tacagtggca gtcgggtctg tttaaccatc ttctcagact gccgctggcg ttttttgaac
     4321 gccgtaaatt aggtgatatc cagtcgcgtt ttggctccct tgacactttg agggccacct
     4381 ttaccacctg tgttgttggg gcaatcatgg acagtattat ggttgtgggt gtttttgtga
     4441 tgatgctgtt atatggagga tatcttacct ggatagtgct cggttttacc atggtttacg
     4501 ttcttattcg tctggtgaca tacggctatt accggcaaat atcggaagaa actcttgtca
     4561 ggggggcccg ggccagctcc tattttatgg aaagcctgta tggtattgcc acggtaaaaa
     4621 tccagggtat ggctgggatt cggggaacac actggcttaa cctgaaaata gatgcgatca
     4681 attcaggtat taagttaacc aggatggatt tgctcttcgg ggggataaat acttttgttg
     4741 ccgcctgtga tcaggtggcg attttatggc tgggtgcaag ccttgtgatc gataatcaga
     4801 tgacaatagg gatgtttgtg gcatttggtt cttttcgtgg gcagttttcg gatcgggttg
     4861 cttcgctgac cagttttctt cttcaactga gaataatgag tctgcataat gagcgcattg
     4921 cagatattgc actacatgaa aaggaagaaa agaaaccgga aattgaaatc gttgctgaca
     4981 tgagcccggt ttcactggaa accactgatt taagctaccg gtatgacagc cagtcagcac
     5041 aggtattcag tggtctgaat ttgtctgtgg ctccgggaga aagtgtggct ataactggtg
     5101 cctccggtgc cggaaaaacc acattaatga aagtattatg tggactgttt gaaccagata
     5161 gtggaaaagt actggttaat ggcacggata tacgtcaact tggaataaat aattatcacc
     5221 gtatgatagc ctgtgttatg caggacgacc gactattttc aggatcaatt cgtgaaaata
     5281 tctgtgggtt tgcagaagaa acagacgacg aatggatgac agaatgtgcc aaagcaagtc
     5341 atattcatga tgtgataatg aaaatgccaa tggggtatga aacgttaata ggtgaactgg
     5401 gggaaggtct ttccggcggt caaaaacagc gtatattcat tgcccgagct ttataccgga
     5461 aacctggaat attatttatg gatgaggcta caagttctct tgatacagaa agtgaacgtt
     5521 tcgtgaatgc tgctataaaa aagatgaata tcacccgggt gattattgca catagagaga
     5581 caactttaag aacagttgat cggattttta gaattacaaa ttaggataat atatcactaa
     5641 aagcagcatt gctgcataat aagctatgct gctttagaaa ttaatcaact tccactacta
     5701 gaagaggatg ttgatgagtt aaatcctgag tttaataaac ctccattgta gtttggatta
     5761 aaacttccaa gtcctatacc cggattatag ttaataccgt ttgatggtat tgttattgtc
     5821 ctatttgaac catcagcaat aactcctcca atatatcctc caattgcact tcctgctata
     5881 tccgcaccag ggatttttgt tgctttacct aaaaaaccac cagccgcagt acctattgca
     5941 acggatgcat ctttatctaa tcctgcacca tgtattaaag caagttcatc ggttgttata
     6001 tttctcatgt tatttcctct ttttattaaa gaaagataaa ataaataaaa acagtacact
     6061 cgcaagtaac gaaagaatta ttttctttat gaaaagataa agggttaatg tgtct
//
LOCUS       DAZGVQ010000240         6044 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000240 DAZGVQ010000000
VERSION     DAZGVQ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.242"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            172..900
                     /gene="gadW"
                     /locus_tag="ACHLAG_004914"
     CDS             172..900
                     /gene="gadW"
                     /locus_tag="ACHLAG_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFP0657998.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1263..4376)
                     /gene="mdtF"
                     /locus_tag="ACHLAG_004915"
     CDS             complement(1263..4376)
                     /gene="mdtF"
                     /locus_tag="ACHLAG_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFP0657999.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4401..5510)
                     /gene="mdtE"
                     /locus_tag="ACHLAG_004916"
     CDS             complement(4401..5510)
                     /gene="mdtE"
                     /locus_tag="ACHLAG_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFP0658000.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5618..5896
                     /locus_tag="ACHLAG_004917"
     CDS             5618..5896
                     /locus_tag="ACHLAG_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658001.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5897..>6044)
                     /gene="gadE"
                     /locus_tag="ACHLAG_004918"
     CDS             complement(5897..>6044)
                     /gene="gadE"
                     /locus_tag="ACHLAG_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFP0658002.1"
                     /translation="LKNKNESNITSTLNISQQTLKIQKFNIMYKLKLRRMSDIVTLGI
                     TSYF"
BASE COUNT         1485 a         1503 c         1517 g         1539 t
ORIGIN      
        1 tatattcgtg ctgcatttac ttattatcaa ttaactgtta tgcaaaacta ctttgtggat
       61 aaattttggt cctaccaaat ctggcagttt tttcgctaag aaacagtctg gcaacatttc
      121 attagtatac tgaaattgaa ataatcgcag tatgaaatat aagggataat catgactcat
      181 gtctgctcgg tgatcctcat tcgtcgttca ttcgatattt atcatgaaca gcataaaata
      241 tcgctgcata acgagagtat cgtgctgctg gagaaaaatt tggcagacga ttttgcgttt
      301 tgttcaccgg atacgcgacg actggatatc gatgagctga cagtttgcca ttacttacaa
      361 aatattcgtc agctaccacg caatttaggg ttacatagca aagaccgtct attaattaac
      421 cagtcacccc ccatgccgct ggtgacggcg atttttgata gcttcaatga atccggggta
      481 aattcaccga tactgagcaa tatgctctac ctttcctgtt tatcgatgtt ttctcataag
      541 aaagaactga tccccttact tttcaatagc atcagcactg tttcaggaaa agttgaacgc
      601 cttattagct ttgatatcgc caaacgttgg tatctgcgcg atatcgcgga aagaatgtat
      661 accagcgaga gtctcatcaa aaaaaagttg caggatgaaa atacctgttt cagtaaaata
      721 ttactcgctt ccaggatgtc gatggccaga cgattactcg agttacgtca aattcctctg
      781 catactattg cagaaaaatg tggctatagc agtacgtcgt actttataaa cacatttcga
      841 caatattatg gtgtaacgcc acatcagttt gcgcaacatt cgccaggtac cttttcctga
      901 catattttgc atttgaatat tggtcaggat ctcacacctg cttcatgtga aactcttccc
      961 tgatgatttc tgccgggcta ccggctagtt ctctttcgca gtttgccagg cggtataggt
     1021 attttcagga ggaaaagact cggcatgttt gggattatta agctgacaat tcatacgatt
     1081 acgggaatgt gggtcagtat tgtgctgttc aaattgatga cgaatggctg gagtggtttc
     1141 tactttcagt gctgtgtact aagcctggta ttcctgacgg ttagctggtt gttgagtggt
     1201 gaatggttag caggaaagag taaggctgaa ccttcacgtt caaccttact ctcatttaca
     1261 cgttacgctt ttttaaagcg ggcaaagaga tgttccacta caacgaaaaa gaccggaacg
     1321 aagtaaattg ccagcactgt tgcggcaaac atcccgccca tcacgccggt acctaccgcg
     1381 ttttgcgcgc cagaaccggc accatgactg ataaccagcg gcagcacgcc gagaataaag
     1441 gccagagagg tcatcaggat tgggcgtaaa cgcatccgcg ccgcttcgat gattgcctct
     1501 atcggcgttt tcccttcttt ttgcatcatc tcaacggcaa attcgacaat caggatggcg
     1561 ttcttggcgg aaagcccgat ggtggtcagc aaaccaacct ggaagtagac gtcattactt
     1621 aagccgcgca gatcggtggc cagtaatgcg ccaacgacgc ctaacggaac aaccaacatc
     1681 accgagaacg gaattgacca gctctcatag agtgcggcga gggcgaggaa caccacgacc
     1741 agtgaaatgg catacagtgc aggagcctgg tttgaggata acgcttcctg atacgacagt
     1801 ccggtccatg agtagccgac gcctgccgga agtttagcga ccaggtctgc cataaatttc
     1861 atagcgtcac cggtactttt cccggccgcc gcttcaccta aaatctccat tgacgggatg
     1921 ccgttgtagc gttccaatcg cggtgaacca taggtccatt cggtagacga gtaggcagaa
     1981 agcggtgcca tcgtgccaga ggcgttgcgt acataccatt ggttgatgtt atccggcaac
     2041 atacggaacg gcgtgcctgc ctggacatac acttttttca cccgcccctg gttgaggaag
     2101 tcgttcacgt agctgctgcc gaaggcggtg gaaattgtct ggttgatatc agacagcgcc
     2161 acacccattg cctcagcttt cgcggcgttg acgttcactt tgaacatcgg cgtatcttcc
     2221 aggccgttcg ggcgtacccc ggtgacctgg tcaggcgatt gcgctgccag tgataacagc
     2281 tcgtttcgtg cctgggttag tttttcgtgc cccaggttac cgttgtccag cagttccata
     2341 tcaaaacctg acgcggtacc cagttcagcc accgcgggta agttgaacgg gaagacgacg
     2401 gctttattga tactgcttaa cgcaatcatt gcccgctgaa tgatcgcggt aaccgagttt
     2461 tcctcaccga cacgttcaga ccacggcttg agactgataa acgccaggcc gttgttttgc
     2521 ccctggccgc tgaagccaaa gccgccaacg gtaaacaccg actgaacatt atctttctct
     2581 tttgtaagat aataatccgt cacctgttgc agcactttcg tggtgttaac catggtggca
     2641 ccggaaggta actgcgcggt ggtcataaat accccctgat cctcttctgg taagaaagag
     2701 gtcggtgtac gcaggaacag caccgccatc ccggcgcaaa tcagcaggta gaccaccatg
     2761 tagcgaccgg tacaacgcaa cagcgagcgg gtgctatcgg tatagtgctg agttgatttt
     2821 tcaaacagcg tgttgaagcg tgcgaacagg gcgttaggtt tgtgaccgcc ttccggcgcg
     2881 gctttcagaa tggtggcgca cagggcaggg gtcaggctca ttgccacaaa tactgaaagc
     2941 agcatggagg agatcagcgt gatggagaac tggcggtaga tctccccggt tgcgccgctc
     3001 ataaaggcca tcggcataaa cactgcggaa agaacaacag caataccgac cagcgcacgt
     3061 tggatctgcc ccatcgattt atgcgtcgct tccttcggtg gtagcttatc ttccgcaatg
     3121 acacgctcga cgttctccac caccacgatg gcgtcatcca ccagtaaccc tatcgccagc
     3181 accatcccga acatagttaa cgtgttgatg gtgaaaccga ccgccgacaa gatcgcaaac
     3241 gtcccgagaa taaccaccgg tacggcaatc gtcgggatga ttgtggcacg gaaattctgc
     3301 aaaaacagat acatgaccag gaagacgagg atgatagcct caaccagtgt tttaaaaact
     3361 tcctgaatag aaatttcgat aaacggcgtg gtgtcgtaag gataaaccgt cttcagactt
     3421 gcggggaaat aggctgataa gcggttcagc tcctctttga ctgcccgcga ggtatccagg
     3481 gcgtttgctc cggtagccag tttgatggcg atcccggcag caggtttgcc gttatagcgc
     3541 gccacggtgg aataatcttc cgctccaagt tcgacgcggg cgacatcacg cagcagcact
     3601 tgcgaaccat cttgctgaac tttcaacagg attttgccaa attcttccgg cgtttgcaga
     3661 cgcgtctgca caatgatcga ggcgtttagt tgctggtctg ccgcctgtgg catgccaccc
     3721 agttgaccac cggaaatctg gttgttttgc accttaatct gggaaataac atcggaaggt
     3781 accagattgt atttattgag tttttgcggg tccagccaga tacgcatggc gtactcggaa
     3841 ccaaagagct gtacgctacc aacgcccgcg gtacggctta gcgggtcttt gatattagac
     3901 gctacatagt ccgcgatatc gtactggttg aggctgccgt tatcagaaat aaacgccgct
     3961 accatcagaa tattactgct cgacttatca acgctaatcc cctgctgctg caccgcttca
     4021 ggtaatgaag gcatagcgag ttgcagttta ttttgcacct gaacctgtgc gatatcagga
     4081 gatgtcccag tctcgaaggt cagagtgata gaggcattac ccgccgcatc actggttgaa
     4141 gacatgtaca tcaggccatc aagcccattc atattttgct caatcacctg agtgaccgag
     4201 tcttctaccg tttgcgcatc ggcacctgga taggtagcgc tgacggtaat ggtcggtggc
     4261 gcaatctgcg gatactgcgc aaccggtaag ttcatgatcg ccagaccacc tgcaagcatc
     4321 ataataatgg caagtaccca ggcaaaaacc gggcgatcaa taaaatagtt agccatgaaa
     4381 gtccccttaa gcctgcaacg ttattgtttc gattcggtgc tggcgttttc ctggctggag
     4441 gaaattgctc gtgctttgat acccggacga atgcgttgca aaccggaaac gatcacccga
     4501 tcgccagcct gcaagccaga ggtgacaacc cactggtcgc cgatggcttt gctggcttca
     4561 atttcgcgta gctgcacaac atcgtcttta tccagaatga gcgccgttgc tttaccctgg
     4621 gcgttgtggg tgacgccttc ctgcggcacc agtaatacat tctggcggct accttcatcc
     4681 actaatgccg tgacgtacat gccaggcagc aagtcaccat ttgggttggg gaaaatcgcc
     4741 cgtaacgtca cggagcccgt ggtttcatcc accgtcgggt cggagaattt cagcgtgccg
     4801 gtctggctgt agcgtttacc attttccaga ttgagctgta ctggcgtact gccctgaacc
     4861 tgtttgattt gcccactggc gacctcttct ttcatgcgta agaaatcttg caccgactgc
     4921 gtgagatcga cataaatcgg gtccagacgt tgtacggtaa ccagcgaatc tgcctgatta
     4981 gcggtaacga gtgcgccgac ggtcaccgac gatttcccgc tgacgcccgt aatcggcgag
     5041 gtgacattcg cgtattgcag attgatcgtc gcctgttcaa cagccgcttt ggcgacggtg
     5101 acattggctt ctgcttcatt caactgggtg cgcgcggtat cgtaatcctg acggctaacg
     5161 tagttggtct tcagcaacga tgcctggcgg ttaaaggtga tgcgggcatt gctggcggta
     5221 gagagcgctt tcgccagcga gcctttggcg gagtttagct cggcctgtaa aggtgcagga
     5281 tcaatctgat acagcgaatc gccctggttc actttatcgc cttcgataaa gttgcgttta
     5341 atgataatac cgcccacctg gggacgtatc tcggcaactt cataaggaac ggttctaccg
     5401 ggcaattcgc tcaacacatt gaccgaaccg ggggagagtg tgacgacacc gacctcaggc
     5461 gtcatggcgg cggcgttttc cgccgatttg tcatcgcagg cggtgagcat cgcgccgcag
     5521 aataacaacg gtattaacag ctttcttctt ctgttcattt tagtccctga aaattcttga
     5581 gaaataacgg ttctttaaat tgcgcgcgag gagagatatg agggatttac agaccagcgg
     5641 catagtgggg ttgtcagcca gtaaggtggg atacaggcac agtgatcgac atggtgaggt
     5701 caacgacaaa aaaaatgttg ccgttctgcc aacagttcct gccagcattc gggcaacgaa
     5761 gggttctact ggtggataca cataccaggg gaataaagtc gttttagcct ctcctttagt
     5821 aatcaccggg ggtaatgaaa tcagcatctg catccctcgt catgccagcc atcaatttca
     5881 gttgcttatg tcctgactaa aaataagatg tgatacccag ggtgacgatg tcgctcatac
     5941 gtcttagttt cagcttgtac ataatgttga atttctgaat ctttaatgtt tgttgcgaaa
     6001 tattgagcgt cgacgtgata ttgctttcat ttttattttt caga
//
LOCUS       DAZGVQ010000241         6026 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000241 DAZGVQ010000000
VERSION     DAZGVQ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.243"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            38..382
                     /locus_tag="ACHLAG_004919"
     CDS             38..382
                     /locus_tag="ACHLAG_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658003.1"
                     /translation="MSTDDVARCKTFFDQGTEIFPGADQKLAYRLCWPGHWLINSRRW
                     FFLDDNSISIQQAVKFALIHQHHWLPATVEQMPPAEMSLALTDYWADYWTQPTEPEKA
                     LVHMTDWQYLNH"
     gene            complement(376..1254)
                     /locus_tag="ACHLAG_004920"
     CDS             complement(376..1254)
                     /locus_tag="ACHLAG_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFP0658004.1"
                     /translation="MKILPVVSTKGGEGKSTQSANLAGFLADAGIRTLLIDGDHAQPT
                     ASNIFPLTYEAPAGLFELLMRTADLTHPDNIISRTAIDGLDLIVSNDPHEQLKTAMLH
                     APDGRLRLRNVLQHPLFLNNYDVIVVDSQGARSVMLEMIVLSATESVVGMVNPVLPDV
                     REFIRGTVNVMENLLPYRELGIPLPKVRTLVNCMDYTALARKTLDELAGIIQTGRYSR
                     QLPEGAVSLLSTQIYDLNIYKQGHAAGQPVHRLEKESTRRSDSALVTMHSLACELFPE
                     WKPAFDGLLENGGTRQ"
     gene            1553..2818
                     /locus_tag="ACHLAG_004921"
     CDS             1553..2818
                     /locus_tag="ACHLAG_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0658005.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRINS
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAERKPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TKEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREECISPFEYAKVNNPLKR"
     gene            3074..4117
                     /locus_tag="ACHLAG_004922"
     CDS             3074..4117
                     /locus_tag="ACHLAG_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP0658006.1"
                     /translation="MTGKLRFEVNDNQGCFIFPETWFGSLLDEFEELIDAYDADEISE
                     TSYINKLRRLARQENDFIDVHAHLAYVFLEQNAPRKALNAALKGLAVGNRLIPEGFSG
                     RIIWIHPDNRPFLRALYAAILANAHLRRHQDAIMLIEKILDYNPEDNHGARWLLGPEL
                     LRTGAHEQARHILQEHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNFSGGPDTAEDYYATYHPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQEKLRERIDETLSEKIVQKCRNMNGEYVWPWILPF
                     SAAGMKHTGIQYQ"
     gene            4260..4489
                     /locus_tag="ACHLAG_004923"
                     /pseudo
     CDS             4260..4489
                     /locus_tag="ACHLAG_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4476..5978)
                     /locus_tag="ACHLAG_004924"
     CDS             complement(4476..5978)
                     /locus_tag="ACHLAG_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP0658007.1"
                     /translation="MNIRKLFCPDNTPRILLFLFFFVISVITTIACGYTEKNATGNVL
                     LLFLLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTADEAAEF
                     TGMIPVYHFLVSAAILVFMVIFWRTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     TTDTLREMQRYKQLSQHRADSWKILPGAPLYDTIVIVTGESVRRDYMSVYGYPVPTTP
                     WLNTAPGLFIDGYTSAAASTVPSLSRTLIYDYEQNPDSGNNVVALAAKAGYSTWWISN
                     QGKLGEHDTRISVIASDAEHTVFLKKGSFASRKTDDMLLLQETERALADKASPKVIFL
                     HMIGSHPNPCDRLHSWPNHYLEQYPRKIACYLASISKLDNFLGQLDGILRRHSRHFVM
                     LYFSDHGLSVSDSANPVHHDGHVQGGYSVPLIITASDITSHQSVSRKISARHFAGIFQ
                     WLTGIRTENIPPFNPLTDEDNEPVMVFNGERNVPADSLKPQPLILPVRGK"
BASE COUNT         1654 a         1451 c         1427 g         1494 t
ORIGIN      
        1 catggcaaaa tattatgaaa actggaaagc ctgaggcatg agcactgacg atgtggcacg
       61 ttgtaaaacc ttctttgacc agggaactga aatctttccc ggggctgacc agaagctcgc
      121 ttaccgactt tgctggcccg gacactggct gattaacagc cgtcgctggt tttttctgga
      181 cgataacagt atcagcattc aacaagccgt taagtttgct cttattcatc agcatcactg
      241 gctcccggca acggttgagc agatgccccc cgcagagatg tcccttgctc tgacagatta
      301 ctgggctgac tactggacac agccgactga gccggagaag gcgctggttc acatgactga
      361 ctggcagtac ctgaatcact gacgagttcc tccgttttcc agcagcccgt caaacgccgg
      421 tttccactca ggaaagagtt cacaggccag gctgtgcatc gtgacaagcg cagaatcact
      481 gcgacgtgtg gattcttttt cgagacgatg aacgggttga ccagccgcat gtccctgctt
      541 gtaaatattc aggtcgtaaa tctgtgtgga aagaagggaa acggcacctt caggtaactg
      601 tctgctgtaa cgaccggtct gaataattcc ggccagctca tccagcgtct tcctggccag
      661 cgcggtatag tccatacagt taaccagcgt gcgtacttta ggcaggggta tcccaagttc
      721 acggtagggc agcaggttct ccatgacgtt gacagtaccg cgaataaatt cacgaacatc
      781 gggaagaacc ggattgacca tcccgacaac cgactcagtc gccgacagca ctatcatctc
      841 cagcatgacg gagcgcgccc cctgagaatc aacgacaatg acatcgtagt tattcaggaa
      901 aagtggatgt tgcagaacat tccggaggcg aaggcgtccg tccggggcgt gaagcatcgc
      961 ggtttttaac tgttcatgag gatcattgga gacaatcaga tcaaggccat cgatagccgt
     1021 gcgggaaatg atattatcgg gatgagtgag gtcagccgta cgcataagaa gttcaaaaag
     1081 gccggctggc gcttcatatg tcagcggaaa gatattactg gctgtgggtt gagcatgatc
     1141 tccgtcaatc agcagggtac gtatacctgc atcggccaga aaaccagcaa gatttgcgga
     1201 ttgtgttgat ttaccttcac cacctttggt ggagacaacg ggcagtattt tcattagtac
     1261 acacctcaat ggtatgtaac caactgacac gaccgtttag gacacgggga ttgaaaatcc
     1321 ccgtgtcctt ggttcgattc cgagtccggg caccaaattc atatcaacgg acctccacgg
     1381 aggtccgttt ttcattccag gacgccacga tttaagcgtc ctgcctccaa atcaattcta
     1441 ccgaactcaa ccagattccc cccacatcaa cctcattgtg tgggtataat tgtgggcata
     1501 ccccagttcg acagaatttg taccctcttt aacgcattca gaaggctgaa ctatggcact
     1561 gactgacgca aaaatccggg cagcaaagcc tactgacaag gcttataaac tcactgacgg
     1621 ggctggcatg ttcctgctgg tacatcctaa tggttcccgt tactggcgtc tccgttatcg
     1681 tatcctgggt aaggagaaga ctctggcact tggtgtatac ccggaggttt ctctctccga
     1741 agctcgtaca aaacgggatg aggcccgaaa actgatttcg gaggggattg acccttgcga
     1801 acagaaaaga gctaaaaaag tagtccctga tttacagctc tcttttgaac atattgcacg
     1861 acgctggcat gccagtaata aacaatgggc acaatcacac agcgataaag tactcaaaag
     1921 cctcgaaaca cacgttttcc cctttatcgg caaccgggat atcacaactc tcaatacccc
     1981 ggatctgctt atccctgttc gtgctgcaga agctaaacaa atttatgaaa tcgccagtcg
     2041 tctgcagcaa agaatatctg ccgtaatgcg ttatgccgta cagtctggca tcatcagata
     2101 taatcctgct ctggatatgg ctggcgcatt gactacggta aaacgccagc atcgccccgc
     2161 tcttgatctt tcacgtctgc ctgaacttct gtcgcgtatt aacagttata aaggtcagcc
     2221 tgtcacccgg cttgccgtta tgctgaattt actggttttt attcgttcca gtgaactcag
     2281 atacgcccgc tggtctgaaa ttgatattga caatgccatg tggactattc cagccgaacg
     2341 caaacctctg ccaggggtaa aattctcaca ccggggctcc aagatgcgaa caccacatct
     2401 tgtgccactc agcaaacagg ctgtagccat actgacagaa cttcagacat gggctggtga
     2461 aaatggtctg atctttacgg gagcacatga cccgcgtaaa ccaatcagtg aaaataccgt
     2521 aaataaggcc ctcagagtga tggggtatga cacaaccaag gaagtctgtg gccatggatt
     2581 ccgggcgatg gcgtgcagtg cattgattga atccggctta tggtcaagag atgcggttga
     2641 acgtcagatg agtcatcagg agcgcaacgg agtacgtgcc gcgtatattc ataaagcaga
     2701 acatctggaa gaacgtcggc ttatgctgca atggtgggca gattttctgg atgcaaacag
     2761 ggaggagtgt atcagtccgt ttgaatatgc aaaagttaac aatccactga aaaggtgact
     2821 gaacaccatt tacacacatg ctcttcctct taaggggaag agcacccgcc tcaatcaatt
     2881 tcttttttat gccggcggaa ccatcagcca ggctgaggtt ctcattcaat aactcaacgt
     2941 cttaccacag aacaggattc actgtattat catgggcaac gttaatcccg gacaataaaa
     3001 ggacaccgat gccaggcatc acaattttta tagcctgcag attatggtcc atcctttttt
     3061 acaggttcag aatatgactg gcaaattacg ctttgaagtg aacgataacc agggctgttt
     3121 catatttccg gaaacctggt ttggctcgtt gctggatgag tttgaagaac tgattgatgc
     3181 atacgatgcg gatgaaatca gcgaaacaag ttacatcaac aaactcaggc gccttgcccg
     3241 acaggagaat gactttattg atgtacacgc tcacttggca tacgtatttc tggagcagaa
     3301 tgccccgcgt aaggcgctga atgctgccct taaaggactg gcagttggca atcgtcttat
     3361 ccctgaaggt ttcagtggca gaatcatctg gatacatcct gacaaccgac cttttcttcg
     3421 tgccctctat gcggcaatcc tggcaaatgc acatttacgg cgtcatcagg atgccatcat
     3481 gctcattgaa aaaatactgg attacaatcc cgaagataat catggtgcac gctggctact
     3541 gggaccggaa ctgttacgca ctggcgctca tgaacaggca cgccatatac tgcaggaaca
     3601 tgcagatgaa ttttcacctt actggtatga actcggcctt ctgcattttc ttaatgggga
     3661 actggtaaaa gctgcaactg catttcgtcg cggctttgcg gcaaatactt acatcgcaga
     3721 aatactgtgc ggaaatctgc atccatttcc acttgctgta tggcataact tttcaggcgg
     3781 tccggatact gccgaagatt attatgccac ataccacccg ttatgggggc aatatccgga
     3841 agcattgctg tttgtaaact ggctctataa tcattcctct gtgctgcatg aacgggcaga
     3901 aatcattaaa tgcgccgaaa tgctgatgca ggaagatgat ttcgaaatat gcgaaagtat
     3961 tttaagacag caggagaagt tgcgtgaaag aattgatgag acgctttctg agaaaattgt
     4021 acagaaatgc agaaatatga atggtgaata tgtctggccc tggatattgc cgttttcagc
     4081 ggcaggcatg aaacatactg gcatacagta tcagtagata ttgcattagt gtatcctgca
     4141 cacaagtaat aatttatcca ccaataataa cactgttaat gtccccttcc cctggttgtc
     4201 agccaggggt tatcttctga atatttcttt tgaaaaggat aacacagtaa attattttta
     4261 tgaattatcc catggactca ttaacaccct ttcataatgc tttattgtca aacacgttat
     4321 ggctgacatc aaaaaaaccg gatttcctct gccagcgggt aatcacctcc ccggtgtttt
     4381 cggttggtct ggttactcca gtctggtaat tagcaagata atcgatataa acagtggaaa
     4441 actcatcgta cataatctgg tgatgaacat tacgcttatt ttcccctgac cggaagaatc
     4501 agaggctgcg gtttcagact gtctgccggt acattcctct ccccgttaaa aaccataacg
     4561 ggttcattat cttcgtctgt cagcggattg aatggcggta tattttcagt acgaatgccg
     4621 gtcagccact gaaaaatacc tgcgaaatga cgggcactga ttttcctgct gacggactga
     4681 tgagacgtga tgtcactggc ggtaataatc aggggaacgc tgtagcctcc ctgcacatga
     4741 ccatcatgat gaacaggatt ggcactatcg ctgaccgaca gcccatggtc agaaaagtaa
     4801 agcatgacaa aatgacggga atgccggcga aggataccat caagctgacc gagaaagtta
     4861 tccagtttac tgatgctggc gaggtaacag gcaatctttc ggggatactg ctccaggtaa
     4921 tgattcggcc aggagtgaag ccggtcacac gggttcggat gagagccaat catgtgcagg
     4981 aaaatcacct tcggagaggc tttatccgcc agtgcacgtt ctgtttcctg taacaacaac
     5041 atgtcatccg ttttacggga agcaaagctg cctttcttga ggaaaacggt atgctccgca
     5101 tcagaagcaa taacagagat gcgtgtgtca tgctctccca gttttccctg attggatatc
     5161 caccatgtgc tgtatcctgc ttttgctgcc agcgccacca cgttgttgcc ggaatcaggg
     5221 ttctgctcat agtcataaat cagtgtccgg ctcagggaag gtacggtact ggctgctgcc
     5281 gatgtatagc cgtcaataaa taaaccggga gccgtattca gccacggtgt ggttggtacg
     5341 ggataaccat ataccgacat ataatccctg cgcacactct caccagtgac aatcacaatc
     5401 gtgtcataca acggtgctcc cggcaggatt ttccagctgt cagccctgtg ctgactcagt
     5461 tgtttataac gctgcatttc acgcaatgtg tcagttgtcc ccacaacggt tcctttaacc
     5521 atccgcaacg gccagctgtt tacagagcat aatacgaaca gcagcagtgc cagccagtta
     5581 cggtgaccgc ggtggtgtgt ccgccagaaa ataaccatga acaccagaat cgcggcactg
     5641 accagaaaat gataaacagg aatcatcccg gtaaactccg cagcctcatc cgccgtggtc
     5701 tgcaacaacg cgacaataaa actgttgttg attttaccgt acgtcatacc cgcaggcgca
     5761 tacagtgcac aacagaacag aaataacaga gctgtaatgg atgtaagggt atttctgtgt
     5821 gcaaggagca gaagaaggaa cagcagcaac acattcccgg tggcattctt ctcagtgtat
     5881 ccgcatgcaa ttgtggttat gacagaaata acaaaaaaga ataaaaacaa taaaatccgt
     5941 ggtgtgttgt ccggacaaaa cagttttctg atattcatcg gagtatatcg gcaacattat
     6001 tatgaagaga ataaaataat aagaaa
//
LOCUS       DAZGVQ010000242         6013 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000242 DAZGVQ010000000
VERSION     DAZGVQ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.244"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            75..2165
                     /gene="iha"
                     /locus_tag="ACHLAG_004925"
     CDS             75..2165
                     /gene="iha"
                     /locus_tag="ACHLAG_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFP0658008.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGMNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLFSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(2383..2533)
                     /locus_tag="ACHLAG_004926"
                     /pseudo
     CDS             complement(2383..2533)
                     /locus_tag="ACHLAG_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            3026..3268
                     /locus_tag="ACHLAG_004927"
     CDS             3026..3268
                     /locus_tag="ACHLAG_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658009.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALAMLSTLCYNLM
                     VTCDIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            3356..3547
                     /locus_tag="ACHLAG_004928"
     CDS             3356..3547
                     /locus_tag="ACHLAG_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658010.1"
                     /translation="MKKTKTHTGLLASKDKTRRVSLYETPTAWCIRGQECYSKSTGRR
                     CGSHDSLSRLRLDSIKPVE"
     gene            3559..3927
                     /locus_tag="ACHLAG_004929"
     CDS             3559..3927
                     /locus_tag="ACHLAG_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658011.1"
                     /translation="MKDNNTTATPEVWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIPPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILARYPHKKDEV"
     gene            3931..4146
                     /locus_tag="ACHLAG_004930"
     CDS             3931..4146
                     /locus_tag="ACHLAG_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658012.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            4143..4415
                     /locus_tag="ACHLAG_004931"
     CDS             4143..4415
                     /locus_tag="ACHLAG_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neurotensin receptor R8"
                     /protein_id="HFP0658013.1"
                     /translation="MTDITDRHTLRRMSWSELFTAAQEAEFQRDYERARIVWSFALHV
                     ATTTINKNLAIAHIRRCDTLLHKSKTVPGNNTGGRSVCLRPQHPRR"
     gene            complement(4724..5555)
                     /locus_tag="ACHLAG_004932"
                     /pseudo
     CDS             complement(4724..5555)
                     /locus_tag="ACHLAG_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            5613..5798
                     /locus_tag="ACHLAG_004933"
                     /pseudo
     CDS             5613..5798
                     /locus_tag="ACHLAG_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1605 a         1425 c         1491 g         1492 t
ORIGIN      
        1 aaatataaat tattctcatt attgtttgta tttgtgtatt gtcttgccgg ttaacatgat
       61 cggagattag taatatgcga ataaccactc tggcttccgt agtcattccc tgtctcggat
      121 tttcagccag cagcatagct gctgcagagg atgtgatgat tgtctcggca tccggctatg
      181 agaaaaagct gactaacgca gccgccagtg tttctgtgat tagccaggag gaattgcagt
      241 ccagccagta ccacgatctg gcggaggctc tgagatcagt agagggtgtg gatgttgaaa
      301 gtggtacggg taaaaccggt gggctggaaa tcagcatccg aggaatgcca gccagttaca
      361 cgctgatact gattgatggt gttcgtcagg gcggaagcag tgacgtgact cccaacggtt
      421 tttctgccat gaataccggg ttcatgcccc ctctggccgc cattgagcgt attgaggtta
      481 tcagggggcc gatgtccaca ctgtatggct ctgatgcgat gggcggtgtg gtgaatatca
      541 ttaccagaaa gaatgcagac aaatggctct cttccgtcaa tgcagggctg aatctgcagg
      601 aaagcaacaa atggggtaac agcagccagt ttaatttctg gagcagtggt ccccttgtgg
      661 atgattctgt cagcctgcag gtacgcggta gcacacaaca gcgtcagggc tcatcggtca
      721 catcactgag cgatacagca gccacgcgta ttccttatcc cacggagtca cagaattata
      781 atcttggtgc acgtcttgac tggaaggcgt cggagcagga tgtgctctgg tttgatatgg
      841 ataccacccg gcagcgttat gataaccggg atgggcaact ggggagtctg acggggggat
      901 atgaccggac cctgcgctat gagcgaaaca aaatttcagc tggctatgat catactttca
      961 ccttcggaac atggaaatcg tatctgaact ggaacgagac agaaaataaa ggtcgtgagc
     1021 tcgtacgcag tgtactgaag cgcgacaaat gggggcttgc cggtcagccg cgggagctta
     1081 aggaatcgaa ccttatcctg aattcattac tgcttacccc tctgggagaa tctcatctgg
     1141 ttacggtggg gggcgagttt cagagctcgt ccatgaaaga cggagttgtc cttgccagca
     1201 caggtgaaac tttccggcag aaaagctggt cggtatttgc tgaggatgag tggcatctca
     1261 cggatgcact tgcgctgact gcgggcagcc gctatgaaca tcatgagcaa ttcgggggac
     1321 acttcagtcc gcgtgcatat ctggtctggg atgtggcaga tgcctggacg ctgaaaggcg
     1381 gtgtgaccac gggatataag gcacccagaa tggggcagct acataaaggg attagtggtg
     1441 tgtccgggca gggaaaaaca aatctacttg gtaaccccga cctgaagccg gaagagagcg
     1501 tcagttatga ggctggggtg tattacgata accccgccgg tatgaatgcc aatgtcacag
     1561 gttttatgac tgacttctcc aacaagattg tctcttattc cataaatgat aacaccaata
     1621 gctatgtaaa cagcggaaag gcccggttgc acggtgtgga atttgccggc acattgccgc
     1681 tgtggtcaga ggatgtcacg ctgtcactga attacacctg gacccgaagt gaacaacgtg
     1741 atggtgataa caaaggtgcg ccgctgagtt atacccctga acacatggtg aatgcgaaac
     1801 tgaactggca gatcaccgaa gaggtggcat catggctggg tgcccgttat cgcgggaaaa
     1861 caccacgttt cacccagaat tattcgtcac tgagcgctgt acagaagaaa gtgtatgatg
     1921 agaaaggaga atacctgaaa gcctggacgg tggtggatgc aggtctgtcg tggaagatga
     1981 cggatgccct gacgctgaat gctgcggtga ataacctgct caacaaggat tacagtgacg
     2041 tgagcctgtt cagtgccggt aagagtacgc tgtatgccgg tgattacttc cagacgggat
     2101 catcaacaac aggatatgtg atacctgagc gaaattactg gatgtcgctg aactatcagt
     2161 tctgataata acaaaacgct atcactgacg gtagaatacg ttgccactgc aactcctggc
     2221 ggaacagtgg caacgtatta ggttaagtgc atttccgatc cgctaatgag atttcgttac
     2281 caacaactaa tatcgtcaca ggaaatgcac ggattatttt taacttatca tttacatact
     2341 tgtccagagt gtaagcgcac cgtgaaggac gaggggtaaa aattagttta cagagagagt
     2401 gacgttccag ggcaacaact ctttcatgcg gttggcaggc caggtgttga ttacactgat
     2461 cacgtggcgt agccacgttt ccggctcgat tccgttaagt tttgcagcta ccgatcaggc
     2521 tgtacatcac tgccgcacta tcgctcgtca tctcaaagtc cggtccgtca gcaggaaggt
     2581 atcattctct cccgccattt ttccgggggc ccggtcagat aagtcccttt gtctatcgct
     2641 gactcctggc tcataacccg gttagcagaa tgcagggtca ccacctcgcc acgaccgagt
     2701 ccggataagt caattgcacc ttctcaatcg ccattttgtc acaattaagt ttgataaagc
     2761 aggtcacctc tgcactgaat ctgctccagt gcctcattgg tctccgtccc cccctttaaa
     2821 ctgccggata agactgctgc acagcgcctt ccccggaatc aaatcacttt ccctgtccgc
     2881 cacaatccgc ttcatgccac ctcgtatttt gtttcagaga ccggaatctg ccagaaagtg
     2941 actacgcgtg cacatatcgg ttttctgggt tacatgcatc gtgttatttt ctgaaatgca
     3001 aaataataaa aaacaaggac acataatgaa ggatgaaata cacagtacca gcatcaaccc
     3061 ggatgttgtt gagcagtttc tggcccttat gaacacacat tgtaaatcct cagaagaggc
     3121 gctcgccatg ttgagtacgc tttgttacaa cctgatggtg acatgtgata tccattccat
     3181 ggacagccag atgctcaatg ggcatgggat atccatgcaa cttcagggga ttgatattgc
     3241 cgaaaggcca ggtaagaata ttcactaacc tgaacagggt ctgaaaacgc tgtcttttca
     3301 ggcctctgca ggcaaaaact gatgacaaca aaatttctgc atttgtaccc caccgatgaa
     3361 aaagacaaaa actcacaccg gacttctagc cagtaaagat aaaacccgac gtgtcagcct
     3421 ctacgaaacg ccaacggcat ggtgtatcag ggggcaggag tgctacagca aaagtacagg
     3481 acgccgctgt ggcagccacg acagtctgag cagactgcgc ctggacagca ttaagcctgt
     3541 tgaatgagtg gtggcaccat gaaagataac aacacaaccg ccaccccaga agtctggaaa
     3601 atgcgttttc tggccaacgg gacatccggc acatggtttt acactgacga cattgcagtc
     3661 actgacgcgt ggcttcgcgc gcacccggat gatgtggagg ttatccctct ctgccggata
     3721 ccaccccgtc agcattccca tgaacaacat attgaagaac tccgcgcagc cctggcccgg
     3781 atgctccata tgcatcatat gatgctggaa accacgcatt acagggaaaa catcaccgat
     3841 gcccgttgta tccgtgaaat gcatgaagct ccattacagg cgctcaatat tctggccaga
     3901 tacccgcata aaaaggacga ggtgtgacgc atgtggctac tgattttctg gctttcatct
     3961 ccctttaatg tctcttccgg tgatgcccgg cagcccacac tggcggtgca cagccaggca
     4021 tttcataccg aacgagcctg tcgcgatgca ttgcgcgcca ttaaaaaaga gaatgttggc
     4081 gaattttctc tgcggggcgt ttgtgtttct gacggaaata cgaaggagga agctgagagc
     4141 tcatgacgga tattactgac agacatacac ttcgccgtat gtcctggtcg gaactcttca
     4201 cggcagcaca ggaagcggaa tttcagcgtg attacgagcg tgccagaata gtgtggtcat
     4261 tcgccttaca tgttgcaaca accacgatta acaaaaatct ggccatagct catattcgac
     4321 gctgcgatac tttgttgcat aaatcaaaaa cagtacccgg aaacaacacg gggggccggt
     4381 cagtgtgcct gagacctcaa catccccgga gatgactgac attccggact gacgcagttg
     4441 ctgcatatct gtggcaatac gttgtatcca gccagacagg ctatcccgcg tagtgacaat
     4501 taacccgctt tcctgcacca gcttcagccg ggccgtcggg gattttaaca gccggaaata
     4561 tcagtaaaac aacatttcat tatgccatgg tgtacgcggc cagtgtacgc ttacttaagg
     4621 aatgagggat aaattggata ttattacttc actttgcttt tttatgagat tcatcacata
     4681 agtggggttt tactgttaaa taatctgaat ttatttgata tccgtaaagc agatgtctca
     4741 gttcactgtt tccaactttt gttattcctc tggggcgaat gctgtttccg ctggaatatt
     4801 cacctggcac cagtcccaga aaagccatga gctgttcagg attggaaaat cgcgaaaaat
     4861 cacccagttc agcaaccagg gttattgcga ttagctgacc aacaccttta agagcctgca
     4921 actggcagac cagattgcac agagatcact caggtaacag gcgagatatt tcctgatcga
     4981 gttgaagtat gcgatcttca atctgctcca gattctgaca ataatgttga aagcaatctg
     5041 ttgagatagc agaccaaatg attgattaac taaccaggtt cgatgtttgc cggttcagtg
     5101 cttcctggca taacgtttgt ctgttcgtgt cacgttttgc agcggcacgg gctctgatta
     5161 aatcccgcat agcttcatga gtcagctcag ggagccagac tgggggaacc tcaccatccc
     5221 tcagtaacct ggcgagagaa attacgtccc gatggtcatt tttgatcctg tctgccggtg
     5281 atttgggtat acgggaaggg ggcaataacc agacatttta tattcatcac tgtgagttga
     5341 tgatatagcc caaacccaca gggaccacct tcatagcagg aaagaatgtt tccgtaaggt
     5401 ccctgcaatt tctgacataa tcgatgtaca gccaaggctt cgttgttaat cgtaccataa
     5461 taacgaattt cgccaagcca ttcaggtgta gcaatcgcca aggtttcttt atgaacaacc
     5521 agcccgacgt aagctgcaat attttccgaa tccatgatga cctctggaat atcccaccaa
     5581 tgctatagtg agtatataaa ggtcatatca tctacgtgtt cttcggcaac gatcacagtg
     5641 gagagcgtgg agcgctactg tacgggctga ttggcacctg ccgactgaac agtatcgacc
     5701 cagaagcgta tttgcgccat atcctgggcg tactactgga atggccttcc aaccgtgttg
     5761 acgaacgctc gccatggaat gtagtatatt accaataaat aaccgtcaat acggcactcg
     5821 gttaacgctt acaaataaga atagacagga caaatgtaaa ctacatttgc atattctgta
     5881 taacattatc acacaagaga atgacaccaa catttctctg gtgtataatt cttttttgtg
     5941 ctcgtaaaat taatgttgcg gagtaatata cctcctttgt tatatttaaa tcattcaata
     6001 aaacgcctaa ttt
//
LOCUS       DAZGVQ010000243         6007 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000243 DAZGVQ010000000
VERSION     DAZGVQ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.245"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(11..163)
                     /gene="hokA"
                     /locus_tag="ACHLAG_004934"
     CDS             complement(11..163)
                     /gene="hokA"
                     /locus_tag="ACHLAG_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFP0658014.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(489..2558)
                     /gene="glyS"
                     /locus_tag="ACHLAG_004935"
     CDS             complement(489..2558)
                     /gene="glyS"
                     /locus_tag="ACHLAG_004935"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFP0658015.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2568..3479)
                     /gene="glyQ"
                     /locus_tag="ACHLAG_004936"
     CDS             complement(2568..3479)
                     /gene="glyQ"
                     /locus_tag="ACHLAG_004936"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFP0658016.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3574..3873)
                     /locus_tag="ACHLAG_004937"
     CDS             complement(3574..3873)
                     /locus_tag="ACHLAG_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFP0658017.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            4048..5043
                     /gene="wecH"
                     /locus_tag="ACHLAG_004938"
     CDS             4048..5043
                     /gene="wecH"
                     /locus_tag="ACHLAG_004938"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFP0658018.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQSRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(5085..5522)
                     /gene="yiaA"
                     /locus_tag="ACHLAG_004939"
     CDS             complement(5085..5522)
                     /gene="yiaA"
                     /locus_tag="ACHLAG_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFP0658019.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(5568..5909)
                     /gene="yiaB"
                     /locus_tag="ACHLAG_004940"
     CDS             complement(5568..5909)
                     /gene="yiaB"
                     /locus_tag="ACHLAG_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFP0658020.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
BASE COUNT         1495 a         1521 c         1440 g         1551 t
ORIGIN      
        1 ccgaagactc ttactctttt aaattgcagg ctaaaaatgc cgccagctca taactcccct
       61 gtttaatatg caattcacac agtgaatctc ttatcatcca ggtgaaaaat aaaagcgtga
      121 aacaaatcac tattaaagaa agtaatctat atttctgcgg cattcccagc cccctgtgtt
      181 gatttccaac gagtaagagg ctaaccttat ggtgttcagg catagggtag cctcactttg
      241 atttatagtc aggtggggct tttctctgtc tgcctttcgg tgaatacctg agacaaacag
      301 tctcaagcac ccgtggctat tctagcttaa taagtttgtt tcttctcctt gatataatcc
      361 taaaaaaacc tcataaaatt aatatatgag ataatcttta ttcagcagaa gattattaaa
      421 ggttgctgta ttatttagcg ataataaaaa aagcctgcca gatggcaggc tttttaataa
      481 cggcgttatt actgcaacag cgaaatatcc gcaacgcgca ggaacagttc gcgcagtttc
      541 tccagcatgg tcagacggtt gagacgcaat tctttgtcat caaccatgac catcacttta
      601 tcgaagaaag catcaaccgg ctcacgcagc tctgccagtt cgaccagcgc atcctggtaa
      661 cgaccttccg caaagtacgg ctccagcttg tcacgtagca ccacaacctg catcgccagt
      721 ttaatttctt ccggctcttt cagggtagag gcattcacgc ggtcgctcag cacttcgtca
      781 gatttcgcca aaatgttgga gacacgcttg ttcgccgccg ccagtgcagc agctgcttca
      841 agggtacgga agtgcgatac cgctttcatg cgggcatcga aatcagccgg acgagtcgga
      901 cgacgcgcca gtaccgcctg gatggtgtca acggtgtaac cttcgtcctg ataccaggcg
      961 cggaagcgac cgagcataaa gtcgataaca tcatcaacca cgttggcgtt agtcagctta
     1021 tcgccataca gacgcactgc ttcttcggtc agcgtttgca gatcaaggtt gaggttcttc
     1081 tcaacgataa ttcgcagcac gccaagtgcg gcacgacgca gcgcaaacgg gtctttgtcg
     1141 cctttcggat gctggccgat accgaagata cccgccagag tatccatctt gtcagcaatc
     1201 gccagcgcac aggctaccgg gttagacggc aggtcatcac cggcaaagcg cggctgatac
     1261 tgctcattca gcgccaccgc gacatcttcc gcttcgccat cgtgacgcgc atagtgcatc
     1321 cccataacgc cctgggtgtc ggtgaactcg aagaccatgt tggtcatcag gtcacactta
     1381 gacagcaggc ccgcacgggt tgcgtggtta acgtcagcgc caatctgttc agcaatccag
     1441 ccagccagcg cctggatgcg gtcagttttg tcgcgcagcg tacccagctg ttgctggaac
     1501 agcacggttt gcaggcgcgg caggttatct tccagacgtt ttttacggtc ggtattgaag
     1561 aagaactcgg catccgccag acgtggacga acgactttct cgttaccaga gataatttgc
     1621 tgcggatctt tcgattcgat gttggcaacg aagatgaagt tcggcagcag tttgccatcg
     1681 ttcgcataca ccgggaagta tttctggtca cctttcatgg tgtaaaccag ggcttcagcc
     1741 ggcaccgcga ggaatttctc ttcgaatttt gcggtcagaa cgaccggcca ctccaccagc
     1801 gaagccactt cttccagcag gctttcgctt aagtcagcgt taccgccaat cttacgcgct
     1861 gcttcttcgg catcggcttt aatcttcgct ttacgttctt cgtaatcggc gatgactttc
     1921 ccgcgctcac gcagaatttc cggatactga tcggcgttgt cgatggtgaa ttccggttcg
     1981 cccataaagc ggtggccgcg aatcacgcga tcggactgaa tgcccagaat ggttgccgga
     2041 atgactttgt cgcccagcag cagggtcacg gtgtgcaccg gacgcacgaa gtgcacgtcg
     2101 cttgcgcccc aacgcatcag tttcgggatc ggcagtttcg ccagagaagt cgcaaccata
     2161 ttcggcagca gtgcttcggt gctttcgccc ttcacatggg cgcgatacag cagccattcg
     2221 cctttatcgg tagtcagacg ctcagcctgg tcaacggtaa taccgcaacc acgcgcccaa
     2281 ccttctgccg ctttgctcgg tttgccttca gcgtcgaacg cctgggcaat cgccgggccg
     2341 cgtttttcga tttcacgatc cggttgcgct tccgccaggt tagctacttt cagcgccaga
     2401 cgacgcggag cagcaaacca ttgaacggtg ccgtgtgcga ggccagcgtt atccagctcc
     2461 gcagtaaaat tcgcagcaaa ggactcagcc aggctgcgca gtgcttttgg tggcagctct
     2521 tcagtgccga tttccaccag aaaagttttc tcagacatag ccgcctctta cttatctttg
     2581 ttgcacatcg ggaagccgag ggcttcacgg gaagcgtagt atgcttctgc cactgctttg
     2641 gtcagggtgc gaatgcgcag aatgtagcgc tgacgctcgg tgacggagat ggctttacgc
     2701 gcatccagca ggttgaagct gtgggcggct ttcagaatac gctcgtaggc tggcagcggc
     2761 agcggatttt ccagcgccag cagctgctgc gcttctttct cgtactgctc aaagcaggtg
     2821 aacaggaagt ccacatccgc gtattcgaag ttgtaagtgg actgctccac ttcgttctga
     2881 tggaacacgt cgccgtaggt ggttttaccc agcgggccgt cgctccagac taggtcgtaa
     2941 acgctgtcta cgccctgaat gtacatggcc agacgttcca gaccgtaggt gatctcgccg
     3001 gtaaccggtt tacactccag accaccaacc tgctggaagt aagtgaactg cgtcacttcc
     3061 atgccgttta gccacacttc ccagcccagt ccccaggcac ccagagtcgg gttttcccag
     3121 ttatcttcca cgaaacggat gtcgtgaata gtcgggtcca tgcccagctc tttcagagaa
     3181 ccgaggtaca gctcctgaat attgtccggc gatggcttaa tgaccacctg gaactgatag
     3241 tagtgctgta aacggttggg gttttcgccg tagcgaccat cggtcgggcg acgagaaggc
     3301 tgaacataag cagccgccat cggttctggc cccagcgcgc gcagacaggt cattgggtga
     3361 gaggttcccg cgccgacttc catgtccaat ggttgaacaa tggtgcagcc ctggcgagcc
     3421 cagtaatcct gtaaggtcag gatcaagccc tggaaggtcc tggtatcaaa cttttgcata
     3481 ttatttcgtg ctggatacgt gtggatttaa aggaagggat cagtataccc gctggatgga
     3541 agatatacag tacgaaacgg gaaaaagcag ggcttaacgc atggaaagat gcaaaaaatg
     3601 gccatctgcg tcaaaagaac agacaaactg ctctttacgg aaggtataac cgcgcatttc
     3661 atagcttccc tggaactgtt cgaaggcggt gacgtcgatt ttttgctcgc cggtgttata
     3721 acgttttgcc gcctgatcct tgcataacgc ttccatattc aacgaacgca gaggatcaac
     3781 ctttacccgt tgtgctttct gcacgggtga cggtgtagaa caacccacta gcaccataag
     3841 cgccattgcc ggaaagaaag cgttcatcat cattaaatta ccgtctggtt tgcaggtgag
     3901 tcttattatt tatatcggta tagtggctga tatcttacga atttccttaa gcctctataa
     3961 gctgtagtga taaaactcag attattccag tgcgccaact gaatgattta tcggtcagac
     4021 tttttttaac cagatacaga gacacagatg cagcccaaaa tttactggat tgataacctg
     4081 cgagggatag cgtgtttaat ggtggtgatg attcacacca ctacctggta tgtgaccaat
     4141 gctcatagtg ttagccccgt cacctgggat atcgccaatg ttctgaattc tgcctctcgt
     4201 gtcagcgtgc cgctattttt catgatttcc ggctatctct tttttggcga acgcagcgcc
     4261 cagtcgcgcc atttcttgcg tatcggctta tgtctgattt tttatagcgc aatcgcactg
     4321 ctctacattg cgctgtttac ctccatcaat atggagttag cgctgaaaaa cctgctgcaa
     4381 aagccagtgt tttaccactt gtggtttttc ttcgcgattg cggtgattta tctggtttca
     4441 ccgctgattc aggtgaagaa cgtcggcgga aaaatgttgc tggtgctaat ggtggtgatt
     4501 ggcattatcg ctaacccaaa cacagtgccg cagaaaattg acggttttga atggctgcca
     4561 attaacttat atatcaatgg cgatactttt tactacattc tgtatggcat gttgggccgc
     4621 gctataggga tgatggacac acagcataaa gcactgtcgt gggtgagcgc cgcgctgttt
     4681 gcgacggggg tttttattat ctctcgcggg acattatatg aattgcagtg gcgcggaaat
     4741 tttgccgata cctggtatct ttactgtggg ccgatggttt ttatctgcgc aatcgcgcta
     4801 ttgactctgg ttaaaaacac gctggatacg cgtaccattc gcggacttgg cttaatctcc
     4861 cgccattcat tgggtatata cggattccac gccttgatta tccatgcgct gcgcacccgg
     4921 ggaattgagc ttaaaaattg gccaatactg gatattattt ggatcttttg cgcgacgttg
     4981 gcagcgagtt tgttactttc tatgctggta caacgaatcg acagaaacag attagtgagt
     5041 taagtaaaag cccgttcaca ttggactgac cgggcttacg tgaattattc gctgtattct
     5101 tcctgggtaa cctgtgtttc ttttggcggg tttattccgg tatcacgaat attcttctgc
     5161 accgctactg caccaaacaa gcttaagaag aaagccagtc cataaaaacc tttttcactg
     5221 agtaataatg tcgcattcca cagacctacc atcagtaatg caacagagat aataaagaca
     5281 gtcaggcagg tcatataata aatggaagtg gtcggtatgc cttcatactt gtcccgaacg
     5341 gtcttttgat aagacgcagc agaaaacagt cctaacacca gtacggcaaa ataataccct
     5401 ttttcattta actgcatctc tgcattccat agccctaaca gataggtaac gataccacca
     5461 acgagagcta tccatgacac aatactaaag gccggtgaat aggttgatat tttgttgtcc
     5521 atcacagtat tcctttcatt cctgaatgtg taagagcttt atgttgctca taccgagcga
     5581 aacaaacgca tttgaccaaa caagcagaca aaaaaggcga tggggtagat caccatttca
     5641 taagcattga taggtgcatt taaaacaccg ataaacaaga gaccgatagt gattaacgca
     5701 actaactggc agatatgggt aaaaaaatta tccagttgcc cgagtttttc tgcgcgaaga
     5761 tatgcatagt taccaaaagt tgctgtcatt aacacgccaa gaaaatagcc ttttccactt
     5821 agcaatgggc atgagatcca tagcccaacc agataaacca gcgccccgac aacaaataat
     5881 agttttgcca cagtttttga ggttttcata acgatctcca tatctaccag cgatacatta
     5941 cgagtaacca acgaaagaca aaactgaaaa atgccattaa caaatgattt tcagaataaa
     6001 ttcatac
//
LOCUS       DAZGVQ010000244         5840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000244 DAZGVQ010000000
VERSION     DAZGVQ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.246"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            87..1238
                     /gene="yiaY"
                     /locus_tag="ACHLAG_004941"
     CDS             87..1238
                     /gene="yiaY"
                     /locus_tag="ACHLAG_004941"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFP0658021.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1403..2941
                     /gene="aldB"
                     /locus_tag="ACHLAG_004942"
     CDS             1403..2941
                     /gene="aldB"
                     /locus_tag="ACHLAG_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFP0658022.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3160..3303)
                     /locus_tag="ACHLAG_004943"
     CDS             complement(3160..3303)
                     /locus_tag="ACHLAG_004943"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658023.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGAPMVIYGLIRPLTIE
                     TRD"
     gene            3486..3809
                     /locus_tag="ACHLAG_004944"
     CDS             3486..3809
                     /locus_tag="ACHLAG_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP0658024.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            3815..4951
                     /locus_tag="ACHLAG_004945"
     CDS             3815..4951
                     /locus_tag="ACHLAG_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP0658025.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYSQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(4948..>5840)
                     /locus_tag="ACHLAG_004946"
     CDS             complement(4948..>5840)
                     /locus_tag="ACHLAG_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP0658026.1"
                     /translation="VLGIAQANVSKYLADFESKVGLKVFDRTTRQLMLTPFGTALLPY
                     INDMLDRNEQLNNFIADYKHEKRGRVTIYAPTGIITYLSKHVIDKIKDIGDITLSLKT
                     CNLERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYASQRYLEKHPISR
                     PDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNTQSALELARNHL
                     GIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYQPWRVQYVLDEMLREI
                     RHQLAQSQQLRPEQAAESED"
BASE COUNT         1444 a         1398 c         1524 g         1474 t
ORIGIN      
        1 ttttgataat caataataat gatcatgttt tcgaatgaaa tcctcagtaa gctgcccacc
       61 cttttttaca ctttcaggag tgtgttatgg catcttcaac tttctttatt ccttctgtga
      121 atgtcatcgg cgctgattca ttgactgatg caatgaatat gatggcagat tatggattta
      181 cccgtacctt aattgtcact gacagtatgt taacgaaatt aggtatggcg ggcgatgtgc
      241 aaaaagcact ggaagaacgc aatattttta gcgttattta tgatggcacc caacctaacc
      301 ccaccacgga aaacgtcgcc gcaggtttga aattacttaa agagaataat tgcgatagcg
      361 tgatctcctt aggcggtggt tctccacacg actgcgcaaa aggtattgcg ctggtggcag
      421 ccaatggcgg cgatattcgc gattacgaag gcgttgaccg ctctgcaaaa ccgcagctgc
      481 cgatgatcgc catcaatacc acggcgggta cggcctctga aatgacccgt ttctgcatca
      541 tcactgacga agcgcgtcat atcaaaatgg cgattgttga taaacatgtc actccgctgc
      601 tttctgtcaa tgactcctct ctgatgattg gtatgccgaa gtcactgacc gccgcaacgg
      661 gtatggatgc cttaacgcac gctatcgaag cgtatgtttc tattgccgcc acgccgatca
      721 ctgacgcttg tgcactgaaa gccgtaacca tgattgccga aaacctgccg ttagccgttg
      781 aagatggcag taatgcgaaa gcgcgtgaag caatggctta tgcccagttc ctcgccggta
      841 tggcgttcaa taatgcttct ctgggttatg ttcatgcgat ggcgcaccag ctgggcggtt
      901 tctacaacct gccacacggt gtatgtaacg ccgttttgct gccgcatgtt caggtattca
      961 acagcaaagt cgccgccgca cgtctgcgtg actgtgccgc tgcaatgggc gtgaacgtga
     1021 caggtaaaaa cgatgcggaa ggtgctgaag cctgcattaa cgccatccgt gaactggcga
     1081 agaaagtgga tatcccggca ggcctacgcg acctgaacgt gaaagaagaa gatttcgcgg
     1141 ttctggcgac taatgccctg aaagatgcct gtggttttac taacccgatc caggcaactc
     1201 acgaagaaat tgtggcgatt tatcgcgcag cgatgtaatc atcatttcca caacggctgg
     1261 caaattgtca gccgcttttt caactatctc tgtaaccctt gcccgtaaat tcgtgatagc
     1321 tgtcgtaaag ctgttaccga ctggcgaaga tttcgccagt cacgtctacc cttgttatac
     1381 ctcacaccgc aaggagacga tcatgaccaa taatccccct tcagcacaga ttaagcccgg
     1441 cgagtatggt ttccccctca agttaaaagc ccgctatgac aactttattg gcggcgaatg
     1501 ggtagcccct gccgacggcg agtattacca gaatctgacg ccggtgaccg ggcagctgct
     1561 gtgcgaagtg gcgtcttcgg gcaaacgaga catcgatctg gcgctggatg ctgcgcacaa
     1621 agtgaaagat aaatgggcgc acacctcggt gcaggatcgc gcggcgattc tgtttaagat
     1681 tgccgatcga atggaacaaa acctcgagct gttagcgaca gctgaaacct gggataacgg
     1741 caaacccatt cgcgaaacca gtgctgcgga tgtaccgctg gcgattgacc atttccgcta
     1801 tttcgcctcg tgtattcggg cgcaggaagg tgggatcagt gaagttgata gcgaaaccgt
     1861 ggcctatcat ttccatgaac cgttaggcgt ggtggggcag attatcccgt ggaacttccc
     1921 gctgctgatg gcgagctgga aaatggctcc cgcgctggcg gcgggcaact gtgtggtgct
     1981 gaaacccgca cgtcttaccc cgctttctgt actgctgcta atggaaatcg tcggtgattt
     2041 actgccgccg ggcgtggtga acgtggtcaa cggcgcaggt ggggaaattg gcgaatatct
     2101 ggcgacctcg aaacgcatcg ccaaagtggc gtttaccggc tcaacggaag tgggccaaca
     2161 aattatgcaa tacgccacgc aaaacattat tccggtgacg ctggagctgg gcggcaaatc
     2221 gccaaatatc ttctttgctg atgtgatgga tgaagaagat gcctttttcg ataaagcgct
     2281 ggaaggcttt gcactgtttg cctttaacca gggtgaagtt tgcacctgtc cgagtcgtgc
     2341 cttagtgcag gagtcaatct acgaacgctt tatggaacgc gccatccgcc gtgtcgaaag
     2401 cattcgcagc ggtaacccac tcgacagcgt gacgcaaatg ggcgcgcagg tttctcacgg
     2461 gcaactggaa accatcctca actacattga tatcggtaaa aaagagggcg cagacgtgct
     2521 caccggcggg cggcgcaagc tgctggaagg tgaactgaaa gacggctact acctcgaacc
     2581 gacgattctg tttggtcaga acaatatgcg ggtgttccag gaggagattt ttggcccggt
     2641 gctggcggtg accaccttca aaacgatgga agaagcgctg gagctggcga acgatacgca
     2701 atatggcctg ggcgcaggcg tctggagtcg taacggtaat ctggcctata agatggggcg
     2761 cggcattcag gccgggcgcg tgtggaccaa ctgctatcac gcttacccgg cacatgctgc
     2821 gtttggtggc tacaaacaat caggtatcgg tcgcgaaacc cacaagatga tgctggagca
     2881 ttaccagcaa accaagtgcc tgctggtgag ctactcggat aaaccgttgg ggctgttctg
     2941 atataagaag ctggtcgcat tgggtattca ttgcctgatg cgacgcttac gcgtcttatc
     3001 atgcctacgg gaacctgacc gtaggccgga taaggcgtac acgccgcatc cggcataaac
     3061 aaagcgcacg ttgttacaat ttacgtttta aacctacgtg cgcaataacc cagactacgc
     3121 caacagaagt gcgtttctgg ggcgtgtttt gtcaggagtt taatctcgcg tttcgatggt
     3181 gagcgggcga atcaggccgt aaatcaccat tggtgctccg aggccaatca gcaataaacg
     3241 gtagataatt gaaggtttaa tacgcttaat ctgtactgtg gatgttttac tggagttatt
     3301 cattagtttg gccaatagtt ccattatttc aatatgataa gtctcgacct gttgaaaagc
     3361 caggtagcac acttcagaaa gtatataaaa aacaatatgt cgttatataa ataaggaaat
     3421 tgcactgcat catggcattt cataatatgc gttaattgtt cttttttata ttctctgagg
     3481 gaaatatgtt cctggactat tttgcactgg gagtgcttat ttttgtattt ctggtgatct
     3541 tctatgggat tattatttta catgatattc cctacctgat tgctaaaaaa cgtaatcatc
     3601 ctcatgccga cgccattcat gttgctggtt gggtgagtct ttttacgtta catgttatct
     3661 ggccattttt gtggatttgg gccacgcttt accgcccgga gcggggatgg ggaatgcaaa
     3721 accatgactc ctccgttgtg cagctacaac agcgtattgc cgggctggag aaacagctcg
     3781 ccgacatcaa atcctcttct gccgagtaat acttatggat ttactgatta ttttgaccta
     3841 tgtggctttc gcatgggcaa tgtttaagat cttcaaaatt cctgtaaata aatggaccat
     3901 tcccacagcg gccctgggtg gcatatttat tgtcagtggt ttaattctgt taatgaacta
     3961 taaccatccg tataccttca aagcgcaaaa agcggttatt tctattcctg ttgtcccaca
     4021 ggtgacaggc gtggtgatcg aagtgacgga taagaaaaat acgctgatta aaaaaggtga
     4081 ggtgctattt cgactggacc cgacgcgtta tcaggcgcgg gtggatcggc tgatggcgga
     4141 tatcgttacc gcagaacata aacagcgggc gttgggcgca gagttagatg agatggcggc
     4201 gaatactcag caggcaaagg ccacgcggga taaattcgct aaagagtatc agcgttacgc
     4261 acgcggcagc caggcgaaag taaacccgtt ttcagaacgc gatattgatg tggcgcggca
     4321 aaattatctg gcgcaggaag cctccgtaaa gtcatcggcg gcggaacaaa aacagatcca
     4381 gagccagctg gatagcctgg tgttgggtga acattctcaa atcgccagcc tgaaagcgca
     4441 gctcgcggaa gcaaaatata accttgagca gacgatagtg cgtgcgccga gcgatggtta
     4501 tgtgacccag gtgctgattc gtccgggtac ctatgccgcg tcgctgccgc tacgtccggt
     4561 gatggtgttt atacccgatc agaaacgaca aattgtggcg cagttccgtc agaactcctt
     4621 gctgcgactg gcccctggcg atgatgcgga agtggtattt aacgctctgc caggtaaggt
     4681 attcagcggc aagctggcag ccattagtcc agctgttccc ggcggagctt atcagtcgac
     4741 cggcacctta cagacgttaa acacagcgcc gggttcagat ggcgttatcg cgaccattga
     4801 actggatgag cacactgatt tgagcgcttt accagacggt atttactccc aggtggcggt
     4861 ctactctgat catttcagcc atgtctcggt gatgcgcaaa gtgctgttac gcatgaccag
     4921 ttgggtgcat tacctttatc tcgatcatta atcttcgctt tccgccgctt gttcaggccg
     4981 cagttgctgc gactgagcaa gctggtggcg aatttcacgc agcatttcat ccagcacata
     5041 ctggacccgc cagggctggt attcccgttt acggaagata gccaccagat ccagccacca
     5101 ttcatacggg tgctgaaagc agggcaccag cgtgccgtct tgcaggtcgc tctgcacact
     5161 tttgtctggc gcaaacacaa tccccaggtg atttcgtgcc agctccagcg ccgattgcgt
     5221 gttatcgcaa acgtaatttc ccttcacgcg ataatcccgc acctctttac tcccggcgac
     5281 gttgaagcgc cagatattcg cgtcgtcaat catcatcgag tcgatcaaaa tacaggagtg
     5341 atgttcgagt tcgtcaggac ggctaatagg atgtttctca agatagcgct ggctggcata
     5401 agcggttacg gcatattggg taataaaact ggcaaccagc gattcatctt ttggtggagc
     5461 gtagctgatt aaaacatcac aatcatcagg aaattcgacg ccttcataaa aggcattacg
     5521 ctcaagattg caggttttta gcgataaggt gatatcaccg atatctttaa ttttatcgat
     5581 tacatgtttg gataaatagg taattatgcc ggttggggcg tagatagtga cccgaccacg
     5641 tttctcatgc ttataatctg caataaaatt attaagttgc tcgtttctat ccagcatgtc
     5701 gttgatgtac ggtaacagcg cggtgccaaa aggtgtgagc atcagctgcc gggtggttcg
     5761 gtcaaagact tttaaaccca cttttgattc aaaatcagca agatatttgc tgacgttggc
     5821 ctgtgcgatg ccaagtacag
//
LOCUS       DAZGVQ010000245         5780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000245 DAZGVQ010000000
VERSION     DAZGVQ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.248"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..800
                     /locus_tag="ACHLAG_004947"
     CDS             <1..800
                     /locus_tag="ACHLAG_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP0658027.1"
                     /translation="SNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREGM
                     PESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAHRVDVTAYAAPEQLMPVVFSTERNSRTLNLLLCKGPEEMLVGFVRLEDGLRP
                     VLALPSPDYSHLMVSTITENGIHLAGYGEAINRDADTPYPPEPKLMQFRLKGCQDTLF
                     AAINKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHYRGMSM"
     gene            complement(1064..2182)
                     /locus_tag="ACHLAG_004948"
     CDS             complement(1064..2182)
                     /locus_tag="ACHLAG_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0658028.1"
                     /translation="MAKLYLYLVLAASLSALSTDMIVPALPELQKAFNADYSLIQLSI
                     SGFLIVFAFSQLISGTIGDYFGKVRVMTICLIIFLIGSIVCFMANNLNMLLLGRILQA
                     IGAGAGPVISKAIAKESFPPLKLKRALSDISSTSAIIPLIAPLAGALILDYYNWNVIF
                     IVMGAFSILTILLSPKESNKQEKKTHQDNVKFVTKDFICGTILVSLMLSSLFCYISIS
                     PALFMSDYGLNTFNYSVVFSLSVLFFIIGNQLSKIEKISNPWILFPLNAISVTPFLIL
                     NDAFIICVIGAMIFNLTLGAYYPIANFISLQINGSRTGLAASITGFTQTVSAGTISFL
                     SVKISDNGVSLGTTLSISCLILALLSIFVTIIIGNNKK"
     gene            2588..2863
                     /locus_tag="ACHLAG_004949"
     CDS             2588..2863
                     /locus_tag="ACHLAG_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658029.1"
                     /translation="MAYDEHSIRVMSADEIEQRFDWLRLENLAKEHRLPVDWVRRGFE
                     ACWRLGIGPDYFIDRYIFKRDVPLVPEFEVVFREIVNENRYRDRMRF"
     gene            complement(2972..3673)
                     /locus_tag="ACHLAG_004950"
     CDS             complement(2972..3673)
                     /locus_tag="ACHLAG_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP0658030.1"
                     /translation="MSDLIAYKSNALVEASYKLTLQEQRFLLLCISRLKSGADAASSE
                     LQKNLIITAEEYFNSFPDMGRKNAEIQLQEAVDRLWDRSILLKDDEKREEFRWIQRRA
                     QYLKGEAKVQITFSDSVMPYLTQLQGQFTKVIIKNISRLSRSYSIRIYELLQQFRSTG
                     ERIISLDDFRSMLDICDKYQTYKSLNQQLLKPCIDELNLKSDLLVTVETIKKGRTVVA
                     LHFRFKEDKQIKVPS"
     gene            complement(4203..4481)
                     /locus_tag="ACHLAG_004951"
     CDS             complement(4203..4481)
                     /locus_tag="ACHLAG_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HFP0658031.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRIQPLINELEKLPGPPVKPQKNG"
     gene            4660..>5780
                     /gene="mobQ"
                     /locus_tag="ACHLAG_004952"
     CDS             4660..>5780
                     /gene="mobQ"
                     /locus_tag="ACHLAG_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFP0658032.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDERTGLTHDYTR
                     KRGVDDAVILTPANAPSWCADRSVLWNAVEKAEQRRNSQLAREIELAIPREISRDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFSGKVRDWNDRAL
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTRLAVIQVQENVRKRELSDAARRAAEALNLTIPAENASA
                     DTLREFIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKA
                     RPVASLLSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRT"
BASE COUNT         1580 a         1305 c         1337 g         1558 t
ORIGIN      
        1 tgagtaacag agccggagaa ttcacggtat ggggtgatga gctggcgcat tacccccaga
       61 gtgttcatga cccggtgaat gtttacctgt cgccaggcgg ggctgtgatg gtctcggata
      121 tacgtgaggg aatgccagaa tctcatgaga cgatggcgcg gcctgagcgt gtgagaatgt
      181 attccggtgc gacggtccgg catgtactgg aacagatgcg ccaggggtgg ccctcttacg
      241 gttttcctgc gttgccgcat cactggccgg ataattttta tttcagcgac gaccgcaggc
      301 ctgtagcctc tccgctgccg tcagcacacc gggtggacgt caccgcttat gcggcaccgg
      361 agcaactcat gcccgttgtc ttttcgacag agcgaaacag caggacgctg aatctgctgt
      421 tgtgcaaagg gccggaggaa atgcttgtcg gttttgtgcg actggaggac gggctgcgtc
      481 ccgttcttgc gcttccgtcg ccggattaca gtcatctgat ggtaagtacc atcacagaaa
      541 acgggataca cctggcaggc tacggtgagg ccataaaccg tgatgctgat actccgtacc
      601 caccggagcc gaagctgatg cagttccggc ttaagggctg tcaggacacg ctttttgctg
      661 ccatcaacaa accggaagag atgccggatt atctcttccg tcaactcgga tttaatcaga
      721 cctggcatga atggaagcgg gacgaacagc acaggcaaca acaacgacgc cccggacact
      781 accggggcat gagtatgtaa tgtcagggac agctatacgc cataaaaaat gaaatagatg
      841 gcctctctat aaagagacca tgatatttac taacccagtt cgttaataca ttctataaag
      901 tttttatgta ggctatctat attttctcgg gtatgaatag ctgatattgc acaacgtaga
      961 atcgcctttc cctcttccac tgtaggatac atagcaacgg ttgtagcaaa accacgatca
     1021 tgcatttttt ttgcatatcg ttatagcttt ttcttttgcc aattcatttt ttattattcc
     1081 ctataataat ggttacgaat attgacaata atgcaagaat aagacagcta atacttaaag
     1141 tggttcccaa agaaactcca ttatcgctta tttttactga aagaaaacta attgtccctg
     1201 cgcttactgt ttgagtaaag cctgtaattg atgctgccaa accagttcgt gaaccgttta
     1261 tctgtaatga gatgaaatta gctattggat agtatgcgcc aagagttaaa ttgaaaatca
     1321 ttgctccaat aacacatatt ataaaagcat catttaatat aaggaaaggt gtgactgata
     1381 ttgcgtttag gggaaataag atccatgggt ttgatatttt ttcgattttt gataattggt
     1441 ttccaatgat gaaaaacaaa actgacaggg aaaaaacaac agagtagtta aatgtattta
     1501 atccataatc gctcataaat agtgcaggcg atattgatat gtagcaaaat agtgagctta
     1561 gcattagtga aacgagtatt gtcccgcaga tgaaatcttt agtcacaaat tttacattat
     1621 cttgatgtgt ttttttctcc tgtttattag actctttagg tgacaggagt atggttagaa
     1681 tgctaaatgc acccattaca ataaatatta cattccagtt ataataatca agtattaatg
     1741 ctccggccag aggtgctatt aatggtatta tcgcacttgt tgatgatatg tcagaaagtg
     1801 ctctttttag ctttagtggt ggaaaagact cttttgcaat tgcttttgat attacaggtc
     1861 cggcacctgc acctattgcc tgtagaattc tacccaaaag aagcatattt aaattgttgg
     1921 ccataaaaca tacaatacta ccaataagaa aaataattaa gcatatagtc attacgcgta
     1981 cttttccaaa ataatcacct atggttcctg atatcagttg actaaatgca aatacaataa
     2041 gaaatccact aatacttagt tgaattaatg agtagtcagc attaaatgct ttctgtagtt
     2101 ctggtaacgc aggcacaatc atgtcagttg ataatgccga taatgatgca gcaagaacaa
     2161 ggtaaagata taattttgcc ataattaacc cctgataaaa atatctttat ttatcatatt
     2221 taaaattctt acaccattga aatgttttat attttgagat agcactgcga tagtatttga
     2281 tcagaaacta atgggtactc ccagatgcat attcaggttg atggaagggg ataaaaaatc
     2341 cccgtgcaga atcttccatc cggtgggaga aaacttgtca ttttgacctg ttcgcccttc
     2401 ggaacggtcg aaaccgatcg cgcatcgctt tcgtgcatag ttatgcaggc ccctgaaaac
     2461 gattctgacg cgttttttct gtttagcctg gtgttttctt gtctttttgc gtttttttgc
     2521 gtcagacgcg tctgagagcg ttttaagggg tacgtgcaac tggagttatg gtaaatcgga
     2581 ggtgtttatg gcttacgacg aacattcgat tcgcgtgatg tccgctgacg agatcgagca
     2641 acgttttgac tggttgcggc tggagaacct ggcgaaggag catcgcctgc ccgttgactg
     2701 ggtcagacgg ggatttgaag cctgctggcg actgggtatc gggccggatt atttcatcga
     2761 caggtatatc ttcaaacggg atgtgccact ggtgccggag tttgaggtcg tgttcaggga
     2821 aatcgtgaac gaaaaccgat acagggatcg gatgcggttc tgatatgcct ctgaaaacgc
     2881 ctgtaagcga ttctgacgcg tttttcttat ttgggtgagg gtttttacgt ccatatccac
     2941 ttttttgcgt cagattgcgt tttaggtcgg tttatgaggg gactttgatc tgtttatctt
     3001 ccttgaagcg aaaatgtagt gctacaaccg tacgcccctt cttgattgtc tcaacagtaa
     3061 caagcagatc acttttcagg tttaattcat caatgcaggg ttttaataac tgttggttaa
     3121 gagatttata tgtttgatat ttatcacaaa tatcaagcat tgaacgaaag tcatcaagtg
     3181 aaataatgcg ctcacctgta ctgcgaaact gctggagcaa ctcgtaaatc ctaatgctgt
     3241 aacttcttga taatcttgat atattcttga ttatcacctt tgtgaattgc ccttgtagtt
     3301 gcgttaggta tggcatcaca gaatcagaaa aagtgatctg tacctttgct tcgcctttca
     3361 ggtattgcgc acgacgttgt atccaacgaa attcctctcg cttctcgtca tccttgagaa
     3421 ggatcgatct gtcccacagc cgatcaacgg cttcctgtaa ctgaatctca gcgttttttc
     3481 tgcccatatc agggaatgaa ttgaaatact cctccgccgt aattatcaga tttttttgca
     3541 actccgatga tgctgcatca gctcctgatt tcaatcgact gatacaaagc aacaagaaac
     3601 gttgttcttg taaggtgagc ttataactgg cctcaactaa cgcattggat ttataggcaa
     3661 tcagatcgga catagtgcat ctccgagaaa aaggcaatac acaaaaaata aacataacca
     3721 atacgaaaag gcaatatata ttttttttcc aatccctatt tctgtggata acaccttaaa
     3781 tcatattacc tttaccttaa atcatattac ctaaacacct taaatcatat tacctttacc
     3841 ttaaatcata ttacctttac ctctgtaacg acatgatttt aaaggcaaaa tcggcaccta
     3901 aaaacaagta taaaaacaag tatataaaaa caagatttaa aaacttaacg gcgtgcctgt
     3961 tgaaaactca aagcgcaaaa aaaaaacact aaaaaaaaca atacgttaaa aaaatcagtt
     4021 tgccgcgttc tgcaaaaaca acgtgcaacc ctgtggaaat ccagtattca tgcgggttca
     4081 gagaggatca cctttctgaa aacagtgcgt atgtgccaca ttcagcaact gcacttctgc
     4141 cgcttcctcg cccacttact cgctaacgct cggtcgctgg ggctgcggcg agcggaagga
     4201 gatcaaccgt tcttttgcgg ttttacaggt ggtccgggta gtttctccag ctcgttaatc
     4261 agcggctgta tccggttcac atcggcctgt cttgtgactt cctttcgcag gaactggagc
     4321 aggaacgcac gcagttgtgc ctcttccggc ctccgtaccc ttgccagcat ggctgccccc
     4381 acaatgactt tttgcgccgt gtccaggctc cggctcttcg ccttcaggcg ctgcaatctg
     4441 gcctcagctt cggcaatctt ctgttcgagt gttctgctca ttttgtgact ccgtgcgcgg
     4501 tgaaaaatcg cattttagcg cgtcactggt agttgcaaaa ctgaactggc ataatgcacg
     4561 gcacatcacg aagtgcgcac ttatacaatc tccactccgt ttcgattgtg tgcggtctgc
     4621 gacgctaaaa gaaaacggca aaaaggcatt acggcagaaa tggcaattta tcatctcagc
     4681 atgaaaatca tttcgcgaaa aaacggctac agtgccgttg cttctgctgc ctaccgttcc
     4741 ggctccgtca ttcccgatga gcgcaccgga ttaacccacg attacacccg taaacgcggc
     4801 gttgatgatg cggtcatact cacccctgcg aatgcaccgt cctggtgtgc tgaccgttcc
     4861 gttctctgga atgcggtcga gaaagccgaa cagcgccgta attcccagct ggcaagggag
     4921 attgaacttg ccattccccg tgagatttcc cgcgatgccg cacgggaaac cgttctcgct
     4981 tttgtcaggg aaaactttgt cagccagggc atgattgccg atgtggcgtt ccatcacatg
     5041 gaccggacca atccccatgc gcacatcatg ctgaccacga gagctgtcgg ggaaacagga
     5101 ttctcaggaa aggtcaggga ctggaacgac cgggcactcg ccgagacgtg gcgcgcatca
     5161 tgggctgacc atgcgaacag agcgcttgcg aacgccggct accaggaaga gatagaccat
     5221 cgttcatacg agcgtcaggg actggagaaa gcgccgggcc ttcacctcgg aaaggctgcc
     5281 tgtgcgatgg aaaaacgggg catggaaaca gaacgcggtg agcagaaccg cctgattaac
     5341 agccttaacc tggaaataca ggtttcccgc acaaggctgg cagtcatcca ggtacaggag
     5401 aacgtgcgga aacgggaact cagcgatgct gcacgtcgtg cagcggaagc tcttaacctg
     5461 accattcccg ccgagaatgc ctcagcggat accctgcggg aattcattgc cacgctgccg
     5521 caggaatgcg ggaacgcgtg ggagatgacc ccggagttcc tggcgatgag cgggaaggtg
     5581 aacgacatcg aacgtgaggg gaatgcgctg ctgaaagagc aggccattct cgaaaaggag
     5641 atgaccggac tcaaaaaagc acgccctgtc gcgtcccttc tgtcagagat tcccctgatg
     5701 acatgggctg aaccggaata ccgcaaaaga caactccgtt tctggaaact cgggaaacag
     5761 attgaatctc ttcgccgcac
//
LOCUS       DAZGVQ010000246         5716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000246 DAZGVQ010000000
VERSION     DAZGVQ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.249"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(236..1201)
                     /gene="alx"
                     /locus_tag="ACHLAG_004953"
     CDS             complement(236..1201)
                     /gene="alx"
                     /locus_tag="ACHLAG_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFP0658033.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(1484..2470)
                     /gene="ygjR"
                     /locus_tag="ACHLAG_004954"
     CDS             complement(1484..2470)
                     /gene="ygjR"
                     /locus_tag="ACHLAG_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP0658034.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(2549..3232)
                     /gene="ygjQ"
                     /locus_tag="ACHLAG_004955"
     CDS             complement(2549..3232)
                     /gene="ygjQ"
                     /locus_tag="ACHLAG_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFP0658035.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPERNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(3309..3812)
                     /gene="ygjP"
                     /locus_tag="ACHLAG_004956"
     CDS             complement(3309..3812)
                     /gene="ygjP"
                     /locus_tag="ACHLAG_004956"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFP0658036.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            3897..5033
                     /gene="rlmG"
                     /locus_tag="ACHLAG_004957"
     CDS             3897..5033
                     /gene="rlmG"
                     /locus_tag="ACHLAG_004957"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFP0658037.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            5224..5307
                     /locus_tag="ACHLAG_004958"
                     /pseudo
     CDS             5224..5307
                     /locus_tag="ACHLAG_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            5304..>5716
                     /gene="higA"
                     /locus_tag="ACHLAG_004959"
     CDS             5304..>5716
                     /gene="higA"
                     /locus_tag="ACHLAG_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFP0658038.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMSGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFI"
BASE COUNT         1450 a         1523 c         1436 g         1307 t
ORIGIN      
        1 ccctcagctt atcccttgcg gctaccgggt cgggcgcaag gtaacgtccc tgtaagatgc
       61 gccctcgccg cgcatccatg cgcgtcgccc cggcctccag ggaacgcttc ggcgattttg
      121 acgccaccaa cacccgtgcg gtctttttgt aaagagctat aagccgatta cataattaat
      181 agctcacctt tgtctggaat gagttatctc tagtaatggc ttaatataca gcggatcatt
      241 ctccccgctg cttatcatgc cgataattca cccaggcgtt gataataaac gtcatcacca
      301 gaatgccaaa caccacgccc agcgagacgg cgattggaat atggtagaag tcgacaatca
      361 gcatcttgat accgataaac accagaatca ccgccaggcc atatttgagc atcgagaaac
      421 gctctgctac gcccgccagc aggaaataca tcgcacgcag gccgaggatc gcaaacaggt
      481 ttgaggtcag cacaatgaac gggtcagtgg tcacggcgaa gatagccgga atgctatcca
      541 cggcgaaaat cacgtcgctc aattccacca gaatcagtac cagcatcagc ggtgtggcgt
      601 acagcaatcc attcttacgc acaaagaaat gctcgttgtc gatggtgtcg gtcatgcgca
      661 aatgaccgcg tagccagcgc accagcggct tgtcgccaat gcctgattca tcttcatggg
      721 cgagcgccat cttcacgccg gtaaacagca ggaaggcacc gaagatatac aggatccagt
      781 cgaactgcga aattagccag ctgccagtga agatcatgat ggtacgcaga acaatcgccc
      841 cgagcacgcc atacaccagc acgcggcgtt gtaatgccgc cggaacagag aaatagctga
      901 acaacatcag ccagacaaag acgttatcga ccgccagcga tttctcaatc agataacctg
      961 tgagaaaggc cagtgcctgt ggatccgcaa cggcgcgacc ttcggtttgc accagatacc
     1021 accagaaagc ggcgttaaac agtaacgaca gcgtcaccca gaccagcgac caggccgcag
     1081 cctgtttcat ggtcatggca tgtgccccac gacgcccctg caacaacagg tcgatagcca
     1141 gcataatggc gacaacaaca gcgaatccgc cccatagcaa cggcgtgccg acagtattca
     1201 tagaagttcc ttacacataa aaaaacggcc aacgtcggaa gacgtcagcc gctgcttttt
     1261 atgcatagac ctcgccttcc ggcaaggtct cacttacaac aaaaaaggaa tacgtctgcg
     1321 tattcctttc gggttgcccg gtgaccggat gtggtttttc acacatcgta atgacgatcg
     1381 accggcaatg aagttactcc cctttgcagg taacaaagta tgacagacca ttcagccagt
     1441 caatggtctg ttacatacct tttacactgt tttactttgg caattatagt tttacgctat
     1501 ctgccggaaa aatcacccca gtctggcggc ggatctcggt cagcagtttg gcggtgatgc
     1561 gactgaccgc cagccccgga tgatccacca gatgctcatc caccagggta gcgaacagct
     1621 ctgcttcgta gagcatggta ttaatatgct gcggctgggt gagatcctgc atttggctgc
     1681 cacgcggcac gaagcacact ttctggcatt cagacagttt ttcaatcacc agcgatcctg
     1741 cttcgccctg aatctcgctc gccaggacag aatcactgac ttttgagtgc tgcaaggtga
     1801 cgctgaaatc accgtaatcc atcaccacca cgccgtgggc atctacaccg cttgccagca
     1861 aactggcggt tgcctgcacg cttttcggct caccaaataa cgccaccgcc gacgccaggc
     1921 agtaaaagcc gatatccata attgaaccgt tagagaatga cggattaaag gtgttgggat
     1981 tttcaccgtc caggtaacgt tgatagcgcg aggaatactg gcaatagttg aaaaagactt
     2041 tacgcaattt gccgactttc ggcagcgcct ggcgcagcaa atgaaagttc ggcaggcagg
     2101 cggttttaaa tgcctcaaac agcaccacct gattttcccg cgcacaggca atggcggcat
     2161 ccacttccgc cagattcgac gccagtggtt tctcgcaaat cacatgaatt ttatggctaa
     2221 ggaaaagttg tgtctgggaa aagtgcaggg cattcgggct ggcaatatac accgcgtcaa
     2281 tggcatcgct ttccgccatc gcttccagcg aggtaaacag atgctcgaca gaaaaatcat
     2341 tggcgaagtg ctgagcctgc tcaaggctgc gggaatatac ggcggttaac ttgtatttac
     2401 cgctctcatg ggcggcctcg acgaactggc gagtgatcca gttcgtacca atcacagcga
     2461 aacgtatcat aacgcgtgga tactccataa agggattctg tcgtcaatgt agcacgcctt
     2521 tttcatcttc gacgtgcgca gatacgcatt attcttttgc cggacagcca tgctcactaa
     2581 acggcccaat catgaccgat ggcccgagat atttcggctg gcgatggagg attttggcgt
     2641 caatcacggc gctgacacga gcgagtttct cccgcagttg agtataaaaa ccgtgcttca
     2701 tattgaggtc aggcgcgtta aaaccgatag cgtcgatgct gtactgtttt gccagccaga
     2761 tggcgcgttg gttatgaaat tcttgcgaga taatggtgat atggttttca ccaaaaacct
     2821 ttttggcccg cactaccgaa tccagcgttg agaatccggc atagtcgcag aagatcactt
     2881 tcgccggtac gcctttagcg atcaaagcct tctgcatacc tgatgcttca tcgtaatttt
     2941 tacgtccgtt atcgccactt accagtagcc atttcacttt tccggcgtgg tacaacgctg
     3001 ctgcggtatc aatacgtcgg gtaaagtagc gattacccgg cctggccccc agcaataagc
     3061 ccacgttacg ctccggaact gcgttgacat cgctccaggt cagctgcttg ctggcgttga
     3121 ccataacgcg atcggcaata aaaatggtcg ctcctgctat aagcaacaac aaacagccaa
     3181 tcttaagagt ccggcgagaa aaacaaatac ggagaagaag gcggggctgc atggtcgtta
     3241 tccttaacga aaggggggag gtcgatttta ggcgctaaag aaagaaaaga taacgcctga
     3301 catcattttc agattttgtc ctgcccaagc gacaactgcg ttagccacag ccgggtgtcg
     3361 aactcaagct ggtggtactg cggctccata tggcagcaca actggtaaaa cgctttgtta
     3421 tgctctttct ccttcaggtg cgccagttcg tgcaccacga tcatacgtaa aaacggttcc
     3481 ggcgcgttgc gaaacacagt agcgacgcgg atctctgctt ttgctttcag cttgccgccc
     3541 tgcacacgag aaacagcggt atgtaacccg agcgcgtttt ttagcacatg gatcttattg
     3601 tcatacatca ctttattgat cggcggggca ttgcgcagaa actgattttt tagatcctga
     3661 gtatattgcc agagggcttt atcggtggcg tagtcgtgtg ttcccggata gcgttttgcc
     3721 agcacatcgc ccagacgctg ttcgttaatc agcgttcgca cctgggaaag tagctgctcg
     3781 ggataaccct gaagataagt aagattgctc ataaccgccc acgaaaaaga gaaaaagggt
     3841 atactcacgc acccttttca ggggcaacgt cgaaatttta tcattcagga gggccgatga
     3901 gccacttaga caacggtttc cgttcactga cactacaacg ttttccggcg acggatgacg
     3961 ttaacccgct acaggcgtgg gaagcggcgg atgaatattt gctgcaacag ttggacgaca
     4021 cagaaatccg cggcccggtg ttgatcctga atgatgcctt tggtgcgtta agctgcgcgc
     4081 tggcggaaca taagccgtac agcattggcg actcatacat cagtgaactg gcgacgcgcg
     4141 agaatttacg cctcaacggg attgatgagt cgagcgtgaa gtttctcgat agcaccgccg
     4201 actacccgca acagccgggt gtggtgctga tcaaagtgcc gaaaacactg gcattgctgg
     4261 aacagcaact gcgtgcgctg cgcaaagtgg tcacgtcgga tacacgtatt attgccggtg
     4321 ccaaagcccg tgacattcac acttccacgc tggagctgtt cgagaaagtg ctcggcccga
     4381 ccaccactac gctggcatgg aaaaaagcgc gcctgattaa ttgcactttc aatgaaccgc
     4441 cgctggccga tgcgccgcag accgttagct ggaagctgga aggtactgac tggactatcc
     4501 acaaccatgc gaatgtcttc tcccgcaccg ggctggatat tggcgcacgc ttctttatgc
     4561 aacatctgcc agagaatctc gaaggtgaga ttgtcgatct cggctgtggt aacggcgtta
     4621 ttggcctgac gctgcttgat aaaaacccgc aggcgaaagt ggtgtttgtc gatgaatcgc
     4681 cgatggcggt cgcttccagc cgaatgaatg ttgaaaccaa catgccagag gcgttggatc
     4741 gctgcgagtt tatgatcaac aacgcgctct ccggagtgga gcctttccgc tttaatgctg
     4801 tgctctgcaa cccgccgttt catcagcaac atgcgctgac cgataacgtc gcctgggaga
     4861 tgttccacca cgcccgccgc tgcctgaaaa tcaacggcga actgtatatc gttgccaacc
     4921 gtcacctgga ttacttccat aaactgaaga agattttcgg caactgcacc accatcgcca
     4981 cgaataataa atttgtggtg ctgaaaacag tgaagctggg gcgtcgtcgg taagattggg
     5041 agttacctgc cggatgcggc gttaacgcct tatccggcct acgttcagca cgttactccc
     5101 aggccggata agacgcggca agcgtcgcat ccggcaatgt ctgcccagtt ctggaatcgc
     5161 gcttccataa aacaaaaaat ccctgtcacc atcctctaca ttctcttgtt tagcgttgtt
     5221 caacgtttat gctacatacg tgaagttatg acgcataaag aatacgattt ctttaccgct
     5281 gttcatcgta ctaaggggaa aaaatgattg cgattgccga catcttgcaa gcaggagaaa
     5341 agctaactgc ggtggcacct tttctggcgg gtattcagaa cgaggaacaa tacacccagg
     5401 cgctggaact ggtagatcat ctgctgctca acgatcctga aaaccccttg ctggatctgg
     5461 tgtgtgccaa aataaccgcg tgggaagaat cagcgcccga atttgcggaa tttaatgcca
     5521 tggctcaagc catgtctggc ggtatagccg tgattcgtac ccttatggat caatatggtt
     5581 taaccctttc cgatctgccg gaaattggca gtaaatctat ggtgtcacgc gttttgagcg
     5641 ggaagaggaa attaacgctg gaacacgcta aaaaattggc aacgcgattc ggcatttctc
     5701 ccgccttgtt tattga
//
LOCUS       DAZGVQ010000247         5698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000247 DAZGVQ010000000
VERSION     DAZGVQ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.250"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            125..835
                     /locus_tag="ACHLAG_004960"
     CDS             125..835
                     /locus_tag="ACHLAG_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0658039.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            877..3528
                     /locus_tag="ACHLAG_004961"
     CDS             877..3528
                     /locus_tag="ACHLAG_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0658040.1"
                     /translation="MTAFRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKIALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYYSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDREHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRSGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            3542..4072
                     /locus_tag="ACHLAG_004962"
     CDS             3542..4072
                     /locus_tag="ACHLAG_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0658041.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4085..4588
                     /locus_tag="ACHLAG_004963"
     CDS             4085..4588
                     /locus_tag="ACHLAG_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0658042.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            4647..5561
                     /locus_tag="ACHLAG_004964"
     CDS             4647..5561
                     /locus_tag="ACHLAG_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0658043.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGAITDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
BASE COUNT         1642 a         1235 c         1270 g         1551 t
ORIGIN      
        1 acatttatta caaacgcatt aagtctggaa acccggcgac tcgccaatgt attagcaaca
       61 ttaatcgatt gtctgatgta acggttttcc cgcttatcct cttaacacat ggagaatgca
      121 tcagatgcaa actacgcgta ccccctactc tatttccttt atggcgacag tgttactttt
      181 attgctcttt gcttgccata gcaccgttgc caacgcagct gtcgcactgg gtgcgacgcg
      241 ggtaatttat ccggcaaacc agaaacaggt tttattgcct gtcacgaata atgatcctgc
      301 cagtgtctat ctcattcagt catggataga aaatgcaggt gaccaaaaag acacccagtt
      361 tgtcattacc ccaccgcttt tttccatgca aggtaaaaaa gagaatacgc tgcggataat
      421 caacgctact aatcatcagt taccaggcga ccgtgaaagc ctgttctggg tgaatgttaa
      481 agctattcct gctatggaaa aagaccagaa gaatgagaat acattacagc tggcaattat
      541 tagccgcata aaaatgtttt atcgccccac gaacctggca atggcacctg aagaagcgcc
      601 tgcaatgctt cggtttcgtc gcagcggtag caaactcacg cttattaatc caacgcccta
      661 tttcattacc gtaacaaata tgaaagcagg aaatagcaat ctcccaaaca ccatggtgcc
      721 gccaaaaggt gaagtatcgg tggatatcac gcatgctgca accggggata tcagttttca
      781 gacgatcaat gattacggtg cattaactcc gcgtattaag gcaaccatgc agtaagccag
      841 tgacctaatt aataataaac tcaatcggaa ttaataatga cagcctttcg tgccgcattt
      901 aaggcatatc gtatgcatca ggtgttaata atgcctcgct ttgcacggct taccattgcc
      961 cttggcttag caactgcggt atttcccgtt gacgcagagt attattttaa tccacggttt
     1021 ttaagcaatg atcttgctga atctgtcgat ttatccgcct ttactaaggg acgcgaggca
     1081 ccacctggta catatcgggt cgacatttat ttgaacgacg aatttatgac tagtcgggat
     1141 attactttta ttgcagatga taataacgca gaactcatcc catgcctgag tacagatctt
     1201 cttgtcagtc ttggtataaa aaaaatagcg ttattagaca ataaagaaca ttcggcagaa
     1261 aaacatgtgc cggacaacag cgcatgcaca ccacttcagg atcgtttggc cgacgcatca
     1321 actgagtttg atgttggtca gcaacatttg tcactctccg ttccgcaaat ttatgttggc
     1381 agaatggctc gcggctatgt ttctcctgat ctgtgggaag aaggaataaa tgctgggctt
     1441 ctaaactata gttttaatgg taattctatt aataatcgta gtaaccataa tgcaggaaaa
     1501 tccaactatg catatttgaa tttacagagt ggcatcaaca ttggtagttg gcgactgcgc
     1561 gataactcaa cgtggagtta ttacagtggg agtagcaatt catctgacag caataaatgg
     1621 cagcatatca atacgtcggc tgaacgtgac attattccct tacgctcacg gttaacggta
     1681 ggtgatagtt ataccgatgg cgatattttt gatagtgtga actttcgtgg cctcaaaata
     1741 aattcaacag aagcgatgtt gcccgatagc caacatggtt tcgctccggt gattcatggt
     1801 attgcccgtg gaaccgcaca agtgagtgta aaacagaacg gatacgatgt ttatcagact
     1861 actgttccac ccggcccttt tactattgat gatatcaact ctgcggccaa tggtggtgat
     1921 ctgcaagtaa ccataaaaga ggcagacggc agtattcaga cattatatgt tccttattcg
     1981 tctgttccgg ttctccaacg tgctggatat acgcgttatg cgcttgccat gggggaatat
     2041 cgtagtggaa ataacctgca aagctctccc aagttcatac aaggtagctt gatgcatggt
     2101 ctggaaggaa actggacacc ttatggcgga atgcaaattg cagaagatta tcaggccttc
     2161 aaccttggta ttgggaaaga tttaggactt tttggtgcct tttctttcga tatcacgcag
     2221 gccaatacga cacttgcaga tggcacccgt cacagtgggc aatcggttaa atccgtctac
     2281 agcaaatcct tctaccagac aggaaccaat atccaggtcg caggatatcg ctattctacg
     2341 caaggttttt ataacttatc cgacagtgcc tacagtcgaa tgagtggtta caccgtcaag
     2401 cctcctaccg gagacaccaa tgagcagaca caatttattg attattttaa tctgttctac
     2461 agtaagcgtg gtcaggaaca aataagcatc tctcagcagc ttggaaatta cggtacgaca
     2521 tttttcagtg ccagtcgcca aagttactgg aacacgtcac gcagcgacca gcaaatatca
     2581 tttggattaa atgtgccgtt tggtgatatt acgacttcgc tgaattacag ctattccaat
     2641 aatatatggc aaaacgatcg ggaacattta ctcgctttta cgcttaatgt tcccttcagt
     2701 cattggatgc gtacagacag tcagtcggca tttcgtaatt caaacgccag ttacagtatg
     2761 tcaaacgatt tgaaaggcgg catgaccaat ctatcggggg tttatggcac tctgctgccg
     2821 gataataacc tgaattatag cgttcaggtc ggtaacaccc acggaggtaa tacatcgtct
     2881 ggcaccagtg gttacagttc tcttaattat cgtggagctt atggtaatac taatgtcggt
     2941 tacagtcgga gtggtgacag cagccagatt tattacggaa tgagtggtgg gattattgct
     3001 catgctgatg gcatcacctt tggacagccg ctgggcgaca caatggttct ggttaaggct
     3061 cctggtgctg ataatgtcaa aatagagaac cagaccggaa ttcataccga ctggcgtggc
     3121 tatgccatat taccatttgc gacagaatat agagaaaacc gtgttgctct taacgcgaat
     3181 tcccttgcag ataatgttga actggatgaa accgtggtca ctgtcatccc aactcacggt
     3241 gctattgcca gagcaacatt taatgcacaa atcggcggga aagtattaat gacgttgaag
     3301 tacggtaata agagcgttcc attcggtgca attgtcacac acggagagaa taaaaatggc
     3361 agcattgtcg cagaaaacgg tcaggtttat ctgactggac ttccacagtc agggaaatta
     3421 caggtttcat ggggcaaaga taaaaactca aactgtattg tcgagtacaa gcttcctgaa
     3481 gtttctcctg gtaccttact gaaccagcag acagcaatct gtcgctaagg aacaatagat
     3541 tatgaaatac aataacatta ttttcctcgg tttatgtctg gggttaacca cctattctgc
     3601 tttatccgca gatagcgtta ttaaaattag cgggcgcgtc ctcgattatg gctgcacagt
     3661 ctcatcggat tcgcttaatt ttaccgtaga tctccaaaaa aacagtgcca gacaatttcc
     3721 aacgaccggt agcacaagtc cagccgtccc ttttcagatt acgttaagtg aatgcagcaa
     3781 agggacaacg ggggttcggg ttgcatttaa cggtattgag gatgcagaaa ataatacttt
     3841 gttgaaactg gatgaaggaa gcaatacggc ctccggtttg ggtatagaaa tattggacgc
     3901 aaatatgcgt ccggtgaaac tgaatgacct tcatgccggg atgcagtgga tcccactggt
     3961 accagaacag aacaatattt tgccttactc cgctcgtctg aagtcaactc agaagtccgt
     4021 caatccggga ctggtgaggg cttcggcaac ctttaccctt gaatttcaat aatcagagtc
     4081 agatatgaaa agacttcaca agaggttcct gttagctacg ttttgcgcgt tattcacagc
     4141 aactctccag gccgccgatg tcactatcac tgttaatggt cgggtagtcg ctaaaccctg
     4201 cactattcaa accaaagaag ctaacgttaa tctcggggat ctttatacgc gcaatctgca
     4261 acaacctggt tctgcatctg gctggcacaa tattactttg tcattaaccg attgtccggt
     4321 tgaaacaagt gcagtgacgg caatcgtgac aggttcaact gacaatacgg gttattacaa
     4381 aaatgaaggt actgccgaaa atattcagat agagctgagg gatgaccagg atgctgcgtt
     4441 aaaaaatggc gatagcaaaa cggttattgt tgatgagatc actcgtaatg cacagtttcc
     4501 acttaaggca agagctatca cggtgaatgg aaacgcaagc cagggaacga tcgaggcgct
     4561 aatcaatgtg atctacacct ggcaataacg tcgattcgat tttcttagcc ttctttttaa
     4621 atcaattttt aaccttagga tatatcatgg gtaaaacaat cagtataaag gtcctgttcg
     4681 gtatttatct tctattaatg gcaggcaaag tttttgcctt cagttgtaat gtcgatggcg
     4741 ggtcaagtat tggagcaggt acaacctctg tttatgttaa tctcgaccct gtaatacagc
     4801 cgggccagaa tctggttgta gacttgtctc agcatataag ttgctggaat gattacggcg
     4861 gctggtacga cactgatcat ataaacctgg tacaaggctc agctttcgca ggctccctac
     4921 agtcatataa aggatcgctg tactggaata atgtgaccta ccccttcccg ttgacgacaa
     4981 ataccaacgt attggatatt ggtgataaga caccgatgcc attaccgtta aaactctata
     5041 ttaccccagt tggggcggcc ggtggagttg tcataaaagc gggggaagtc attgcccgta
     5101 ttcatatgta taaaattgca acattaggaa gcggaaatcc gcgtaatttt acgtggaata
     5161 ttatttcaaa taacaatgtt gttatgccaa caggcggctg caccgttgat agtcgtaatg
     5221 tcaccgtgga tctcccggac tttcctggaa gcgcagaaat accccttggc gtatattgct
     5281 caagcgaaca aaagctctca ttttatcttt cgggtgccat cactgacagt agccgacaag
     5341 tgtttgctaa cacagcacct gatgcaacaa aagcatcggg agtgggtgtc actctaatgc
     5401 gtaatggaaa aatactggct acgggagaaa atgtctccct ggggacagta aataaaagca
     5461 aagtacctct gggtttaagc gcaacctatg gacaaacagg aaacaaggtt tcagccggaa
     5521 ccgtccagtc agtcattggt gtcactttta tctatgaata attgtgattt tgaccttagt
     5581 ctatgcgctc cattcacgaa aattgcgcgg ataattattt tgtgaaggct attagcctac
     5641 acctgtaaga aaatccgcgc acaattgaag ccagagtctt attttttata ttgaaaaa
//
LOCUS       DAZGVQ010000248         5689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000248 DAZGVQ010000000
VERSION     DAZGVQ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.251"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            39..179
                     /locus_tag="ACHLAG_004965"
     CDS             39..179
                     /locus_tag="ACHLAG_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658044.1"
                     /translation="MAENGYGLAGLEMGKVKSINQYRLTPGFGGFTPVPYATTECRLP
                     CR"
     gene            285..596
                     /locus_tag="ACHLAG_004966"
     CDS             285..596
                     /locus_tag="ACHLAG_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0658045.1"
                     /translation="MFNVITHPAALNELIELPDNLRGRMARLIERLEQEGNKMKMPHS
                     RVIGGGLFELRVGDKNIARTLYAYATGNEIYLLHAFVKKTQKTPTKAIEIARMRLKEM
                     K"
     gene            596..883
                     /locus_tag="ACHLAG_004967"
     CDS             596..883
                     /locus_tag="ACHLAG_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0658046.1"
                     /translation="MKVKGVPFSQVKQKLLNTPEAVKGYEDADKELEMVEMLYEMREK
                     AGLTKSALAERMGLRPSAISRLESNPLGASMKTLTKYAQACGATINIHAVY"
     gene            complement(1032..2260)
                     /locus_tag="ACHLAG_004968"
     CDS             complement(join(1032..1945,1945..2260))
                     /locus_tag="ACHLAG_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HFP0658047.1"
                     /translation="MIDVLGPEKRRRRTTQEKIAIVQQSFEPGMTVSLVARQHGVAAS
                     QLFLWRKQYQEGSLTAVAAGEQVVPASELAAAMKQIKELQRLLGKKTMENELLKEAVE
                     YGRGKKVDSARALIARGWGVSLVSRCLRVSRAQLHVILRRTDDWMDGRRSRHTDDTDV
                     LLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYRIMRQNALLLERKPAVP
                     PSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDCCDREALHWAVTTGGFN
                     SETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQFARMLGLEPKSTAVRS
                     PESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREY
                     LRQRACNGLSDNRCLEI"
     gene            2887..3833
                     /gene="ompP"
                     /locus_tag="ACHLAG_004969"
                     /pseudo
     CDS             2887..3833
                     /gene="ompP"
                     /locus_tag="ACHLAG_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193612.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpP"
     gene            3926..5425
                     /locus_tag="ACHLAG_004970"
     CDS             3926..5425
                     /locus_tag="ACHLAG_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010892530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP0658048.1"
                     /translation="MRVIMKPLRRTLVLFIFSVFLCGTVSARQIEWQSCMTSPYSDWF
                     GTESPSPELLCGYLSVPLKYTDTGGDMSDENIPLVRLAMTKLPAKSKRKGSIIIISGG
                     PGLPGINPYINFDWPVTNLRESWDIIGFDPRGVGQSFPAINCQQPNQERSVNVSEKQL
                     ILQKINACIHNTGAEVIRHIGSHEAVYDIERIRQALGDKQLTAVAYSYGTQIAALYAE
                     RFPSSIRSIVFDGVVDIDDLNDNFSWKLRQAHSYQETFDRFAAWCARTKSCPLSSDRN
                     QAIHQFHQLLLKLHNSPLTDSRGESISSDDLISLTTELLLWRSSWPTLATAVRQFSQG
                     IVSNEIETALNSSIASEKVSDALGVILCVDQSDEQLSQEQRKSRKKALADAFPAVNFE
                     REQSDLPEFCELWPIHRDLQQTRLKNTVLPSGLLFVAHKYDPTTPWINARKMADKFSA
                     PLLTINGDGHTLALAGTNLCVDEAVVRHLLFPGKSEDITCQGSGTGDTN"
BASE COUNT         1643 a         1192 c         1285 g         1569 t
ORIGIN      
        1 tcgtcgggac cactccgacc gcgcacagaa gctctatcat ggctgaaaac gggtatggct
       61 tagcagggct ggagatgggt aaggtgaaat ctatcaatca gtaccggctg acgccgggct
      121 tcggcggttt tactcctgtg ccatatgcaa caacggaatg ccgccttcca tgccgctgat
      181 gcggcatcaa aactgtactt aacatgtatt tcatttatca gtgaaatttg atatttgcgc
      241 aattttcctg ttctggtgta ttttatcctt tcggaggcta taatatgttt aatgtaatta
      301 ctcacccggc cgcactgaat gagttgattg aattaccaga taatctgcgt ggtcgcatgg
      361 ctaggctgat tgaacgactg gaacaagaag gtaacaaaat gaaaatgcct catagtcgtg
      421 taattggcgg aggacttttt gaactgagag ttggggataa aaatatcgca aggacactgt
      481 acgcttatgc aactggaaac gaaatctatc tgctgcatgc atttgtgaaa aagacgcaaa
      541 agacaccaac gaaagctatt gaaatcgcta ggatgcgcct gaaggagatg aaataatgaa
      601 agtaaaaggt gttccgttca gtcaggtaaa gcaaaaactg cttaatactc cagaagctgt
      661 caaagggtat gaagatgccg ataaggaact ggagatggta gaaatgttgt acgaaatgag
      721 agaaaaagcg gggttaacaa agtctgctct ggctgaacga atggggttac gcccgtcagc
      781 catcagtcgc ttggaaagta atcctttagg agccagtatg aaaactttaa caaaatatgc
      841 tcaggcctgt ggtgcaacca tcaatattca tgctgtctat tgatttgcat gttaacgata
      901 tgtatttcta atcaaaagat tgaaaaacat ctaaaatgga tgtttttcaa cacataaaag
      961 tcagcgggta acgtcaactt aagatacaac gacagtctga atatcgtggg tcaaggacac
     1021 tggatttgcc cctatatttc cagacacctg ttatcactta acccattaca agcccgctgc
     1081 cgtagatatt cccgtggcga gcgataaccc agtgcactat gcggatgcca ttcgttataa
     1141 tgctcgaacg cctctgcaag gttctttgct gccgttaacc cgtctggttt gggcatgaca
     1201 ctgatgtagt cacgctttat cgttttcacg aagctctctg ctatgccgtt actctccgga
     1261 ctccgcaccg ccgtgctctt cggttcaagc cccaacatcc gggcaaactg ccgtgtttca
     1321 ttagcccggt agcatgaacc attatccgtc agccactcta ctggagacgc cggaagctcg
     1381 ttgccgaagc ggcgttccac cgctcccagc atgacgtcct gtactgtttc actgttgaag
     1441 ccgccggtag tgaccgccca gtgtagtgcc tcacggtcac agcagtccag cgcgaacgtg
     1501 actcgcagtt tttctccgtt atcacagcgg aactcgaacc cgtcagagca ccatcgctga
     1561 ttactttctt tcacagccac tctgcctgta tgtgcccgtt tcgatggcgg tacagcaggt
     1621 tttcgctcaa gcaacagcgc attctggcgc atgatccggt aaacacgttt ggcattgatc
     1681 gcaggcatac catcaagttc tgcctgtctg cgaagcagcg cccatacccg acgataacca
     1741 tacgtgggca gctctccgat aacatggtgt atacggagaa gcacatccgt atcatcagtg
     1801 tgacgactgc ggcggccatc catccagtca tcggttcgtc tgagaatgac gtgcaactgc
     1861 gcacgcgaca cccggagaca acggctgact aagcttactc cccatccccg ggcaataagg
     1921 gcgcgtgcgc tatccacttt tttgcccgtc catattcaac ggcttctttg aggagttcat
     1981 tttccatcgt tttcttgccg agcaggcgct ggagttcttt aatctgcttc atggcggcag
     2041 caagttcaga ggcaggaaca acctgttctc cggcggcgac agcagtaaga cttccttcct
     2101 ggtattgctt acgccagaga aataactggc tggctgctac accatgttgc cgggcaacga
     2161 gggagaccgt catccccggt tcaaagctct gctgaacaat tgcgatcttt tcctgtgtgg
     2221 tacgccgtct gcgtttctcc ggccctaaga catcaatcat ctgttctcca atgactagtc
     2281 taaaaactag tattaagact atcacttatt taagtgatac tggttgtctg gagattcagg
     2341 gggccagtct attcattaag ataaatatta tttttctggt ctactcggga aagtcgcgat
     2401 atccttatga aggatggatg atgtttttta tattgagttt taatatgcct ggcatattaa
     2461 accagagata agtatataat atttccattc acagcgtcag atgacaccac attttactcc
     2521 attttcatag cgggttatga tggctctcgg tagctaaatg attttctggc ggaaatacag
     2581 gaagatagca tacaatatca ttttcattct attaactcct taaaataatt atttagtcaa
     2641 tattgagaat cagtgattgc tgaaaataaa cagataaggc taagctgttt ttagataata
     2701 tgaggacaat gaatgttaca attgcactat aactaacagc gcattcatat ctttttgata
     2761 tacctcacaa aaacaaacaa ttaggttaaa caaaagctaa acaaattaag tactgataat
     2821 tgacttaccg gtgtacatca aaagaatcat atacatatgt agaagtgaca gaagggggtc
     2881 attgttatgc aaactaagct tctggcaatt atgcttgcag cacctgtggt attcagttct
     2941 caggaagctt ctgcttctga tttcttcggc ccggagaaaa taagtacaga gattaattta
     3001 ggtacactga gtggcaaaac aaaagaacgg gtttatgagc ctgaagaagg tggacgtaaa
     3061 gtcagccaac tggactggaa atacagtaac gccgccattc ttaaaggcgc cgttaactgg
     3121 gagctgaatc catggttatc tgttggtgca gctggctgga ccactctcaa tagtcggggg
     3181 ggaatatggt tgatcaggac tggatggatt ccgggactcc cggaacatgg acagatgaaa
     3241 gcaggcatcc tgatacacgt cttaattatg ccaacgaatt tgatttgaac gttaaaggct
     3301 ggtttttaaa agaatctgat tatcgccttg ctattatggc aggttatcag gaaagccgtt
     3361 acagttttaa tgctacagga ggaacttata tttacagtga gaatggtggt ttccggaatg
     3421 aaacgggagc gttacctgat aaaataaaag tgattggtta taaacaacat tttaaaattc
     3481 cttatgtcgg tctgacagga aactaccgtt acgataattt tgagtttggt ggtgcattta
     3541 aatacagcgg gtgggtcagg ggatctgata atgatgagca ttatgtaaga caaactacat
     3601 tccgaagcaa agtaaaaaac cagaattact attctgttgc agttaatgcc ggttactata
     3661 tcaccccaga ggcaaaagtg tacatcgagg gtgtatggag tcgtctcaca aataaaaaag
     3721 gggatacatc tctttacgac cgtagtgata atacttcaga gcataataat aacggggctg
     3781 gaattgaaaa ttacaacttc attacgacgg ccggtctgaa gtacacgttt taacaacttt
     3841 aactgtcatc atctccggtg agaattatac cgccggagat tctgtctgtt ggtcagcgta
     3901 ataatttacc aatgcaggag atgcaatgcg tgtgataatg aagcctttaa gaaggacttt
     3961 agtattattt atcttttctg tttttctttg cggaactgtg tctgccagac agatcgagtg
     4021 gcaatcctgt atgaccagcc catacagtga ctggtttggt acagaaagcc cttcacctga
     4081 gttgttatgc ggatatctat ctgtaccgct aaaatatacc gacacaggtg gggatatgtc
     4141 agatgaaaat ataccacttg tcagactggc gatgacaaag ttgccggcaa aaagtaaacg
     4201 taaagggagt attattataa taagtggcgg acctggatta cccggaatta atccttatat
     4261 caattttgac tggccggtca caaatcttcg ggaatcatgg gacattatcg gttttgatcc
     4321 acgaggtgtc ggtcagtcct ttcctgccat aaactgccag caacccaatc aagaaagatc
     4381 ggtgaatgta agcgaaaagc aactaatttt acagaaaatt aatgcctgta tccataatac
     4441 aggagctgaa gtcattcgcc atattggatc tcatgaggct gtatatgata ttgaacgcat
     4501 cagacaagct ctgggagata aacaactgac agctgtagca tattcatacg gtacacagat
     4561 tgcagcctta tatgccgaac gctttccatc cagtatcaga tctatagttt ttgacggtgt
     4621 cgttgatatt gatgatttaa atgataattt ttcatggaaa ctcaggcagg cccacagtta
     4681 tcaggaaacg tttgatcgtt ttgctgcatg gtgtgccagg acaaaaagtt gtccactttc
     4741 ttcagacagg aatcaggcaa tacatcagtt ccatcagctt ttattaaaat tacataattc
     4801 tcctttaaca gacagcaggg gggaaagtat atcttcagat gatctcatat cattaacgac
     4861 agagcttctg ttatggcgct catcatggcc aacacttgca acggccgtac gtcagttctc
     4921 tcagggtatt gtcagcaatg aaattgaaac tgcacttaat tcttccatag catcggaaaa
     4981 agtcagtgat gctctgggtg taatattatg tgttgatcag agtgatgaac aattatcaca
     5041 ggaacaacga aaatcgcgaa aaaaagccct tgctgacgca tttccggccg ttaattttga
     5101 gagggaacag tccgatttac ctgaattttg tgaattatgg ccaattcatc gcgatctgca
     5161 acagacacgc ctgaagaata ctgttttacc atccggttta ctgtttgttg cacacaaata
     5221 tgatccgaca actccctgga taaatgcccg taaaatggca gataaatttt cagctccgtt
     5281 actgactatt aacggtgacg ggcatacgct ggcgcttgct ggcactaatt tatgtgtgga
     5341 tgaggctgtt gttcgtcacc tcctgtttcc ggggaaatct gaagatataa cgtgtcaggg
     5401 aagtggtacc ggagacacta actgatatta aataaaatat tactcccgac attaaataac
     5461 atcagtgctt tgatgtcagg gcctgctaac cctcttttga tatcagttga gcgcaggaaa
     5521 cgccagttaa gaggggacag gacttaggat aaataagaga gtggcaatca tgagtaagat
     5581 tcatgtacat gcaggttacg gaaagaggat atcaggcaat gaataaggca ctaatataat
     5641 atcatatctc agacttgata cattttagtt acatatattt tcttatttt
//
LOCUS       DAZGVQ010000249         5648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000249 DAZGVQ010000000
VERSION     DAZGVQ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.252"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..175)
                     /locus_tag="ACHLAG_004971"
     CDS             complement(<1..175)
                     /locus_tag="ACHLAG_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HFP0658049.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDL"
     gene            479..1411
                     /gene="rfaD"
                     /locus_tag="ACHLAG_004972"
     CDS             479..1411
                     /gene="rfaD"
                     /locus_tag="ACHLAG_004972"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFP0658050.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1421..2467
                     /gene="rfaF"
                     /locus_tag="ACHLAG_004973"
     CDS             1421..2467
                     /gene="rfaF"
                     /locus_tag="ACHLAG_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFP0658051.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2471..3463
                     /gene="rfaC"
                     /locus_tag="ACHLAG_004974"
     CDS             2471..3463
                     /gene="rfaC"
                     /locus_tag="ACHLAG_004974"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFP0658052.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAILGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3460..4668
                     /gene="rfaL"
                     /locus_tag="ACHLAG_004975"
     CDS             3460..4668
                     /gene="rfaL"
                     /locus_tag="ACHLAG_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFP0658053.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(4704..>5648)
                     /locus_tag="ACHLAG_004976"
     CDS             complement(4704..>5648)
                     /locus_tag="ACHLAG_004976"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP0658054.1"
                     /translation="RIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVVHNSMK
                     LYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIEIVPN
                     GIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHKNLK
                     LVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADLVVIPS
                     QFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILKTL
                     ANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
BASE COUNT         1567 a         1238 c         1266 g         1577 t
ORIGIN      
        1 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
       61 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
      121 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
      181 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
      241 tattatccat tcaaatgctt ccgttattta tcatacatcg gaatattgat actaaagcac
      301 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
      361 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
      421 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
      481 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaaag ccctgaatga
      541 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
      601 cctggtggat ctgaatatcg cagactatat ggataaggaa gacttcctga tccagattat
      661 ggctggcgaa gagttcggcg atgtcgaagc gattttccac gaaggcgcgt gctcttccac
      721 caccgagtgg gacggcaagt atatgatgga taacaactat caatactcca aggagctgct
      781 gcactactgc ctggagcgtg aaatcccgtt cctgtacgct tcttccgcag ccacctacgg
      841 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga acgtctacgg
      901 ttactcaaaa ttcctgtttg atgaatatgt tcgccaaatc ctgccggaag cgaactcgca
      961 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     1021 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     1081 cgaaggtagc gagaacttta aacgcgactt cgtctatgtt ggcgatgtgg cagatgtaaa
     1141 cctgtggttc ctggaaaatg gcgtttccgg catcttcaac ctcggcaccg gtcgtgcgga
     1201 atccttccag gctgtagctg atgctacgct ggcttatcac aagaaaggcc agatcgaata
     1261 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     1321 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     1381 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     1441 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     1501 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     1561 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     1621 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     1681 acctaactcc ttcaaatccg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     1741 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     1801 ctggccgcta atggtggaac gctatatagc gctggcctat gacaaaggca ttatgcgcac
     1861 agcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     1921 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gtttttgccc
     1981 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg agctggcaaa
     2041 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tcggcgaaag atcatgaagc
     2101 aggcaatgag attcttgccg ctttaaatac cgagcagcag gcatggtgtc ggaacctggc
     2161 gggggaaaca cagcttgatc aagcggttat cctgattgca gcctgtaaag ccattgtcac
     2221 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     2281 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     2341 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     2401 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     2461 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     2521 gttgcccgca ctcactgatg cccagcaggc aatcctaggg attaagtttg actgggtggt
     2581 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     2641 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     2701 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     2761 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     2821 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     2881 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     2941 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     3001 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     3061 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     3121 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     3181 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     3241 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     3301 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     3361 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     3421 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     3481 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     3541 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     3601 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     3661 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     3721 cctgatgcag gtgctagcat gaaggaaatt ttcaaagcta ttattgagaa tactttacta
     3781 tgcacaatag caataccgat catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     3841 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     3901 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     3961 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     4021 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     4081 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     4141 caatggaagt tactattagt aggagtaatg gttgccatca ttgcattgtc ggttattttc
     4201 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     4261 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     4321 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     4381 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     4441 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     4501 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     4561 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     4621 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     4681 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     4741 tgagttacgc catcccaact gtagtgttca aacacaaagt cctgaccttg ctttgcaact
     4801 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     4861 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     4921 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     4981 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatgcatc
     5041 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     5101 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     5161 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     5221 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggggag
     5281 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     5341 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     5401 tataaactag gcactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     5461 tgcatatgta taaccactct tgcctgagga gctcgcttac gaatttgagt gtatagcttc
     5521 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     5581 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     5641 tatatgcg
//
LOCUS       DAZGVQ010000250         5530 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000250 DAZGVQ010000000
VERSION     DAZGVQ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.253"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            68..2584
                     /locus_tag="ACHLAG_004977"
     CDS             68..2584
                     /locus_tag="ACHLAG_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658055.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFVLLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRSIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAIRQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLDNAWYERNMAGFNEKLRES
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            2660..3115
                     /locus_tag="ACHLAG_004978"
     CDS             2660..3115
                     /locus_tag="ACHLAG_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFP0658056.1"
                     /translation="MIHLFKTGMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            3150..3278
                     /locus_tag="ACHLAG_004979"
                     /pseudo
     CDS             3150..3278
                     /locus_tag="ACHLAG_004979"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3234..3422)
                     /locus_tag="ACHLAG_004980"
     CDS             complement(3234..3422)
                     /locus_tag="ACHLAG_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658057.1"
                     /translation="MSEKPSTGEPRIKLFSGISFRVPAASASLTFRVADNPTEMHSRV
                     ILDKHIAVSGYDSNRLPV"
     gene            3526..4347
                     /locus_tag="ACHLAG_004981"
     CDS             3526..4347
                     /locus_tag="ACHLAG_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP0658058.1"
                     /translation="MRLVSRFGYAANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            4347..4592
                     /locus_tag="ACHLAG_004982"
     CDS             4347..4592
                     /locus_tag="ACHLAG_004982"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658059.1"
                     /translation="MKHIPEGVIQAPESPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWPSVCDRNGYAYGCPEFQPNIIHP"
     gene            4686..5159
                     /locus_tag="ACHLAG_004983"
     CDS             4686..5159
                     /locus_tag="ACHLAG_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP0658060.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFSLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            5175..>5530
                     /locus_tag="ACHLAG_004984"
     CDS             5175..>5530
                     /locus_tag="ACHLAG_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFP0658061.1"
                     /translation="MQQLSFLPGAMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNKLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKA"
BASE COUNT         1312 a         1462 c         1487 g         1269 t
ORIGIN      
        1 ggggaaaacg tttcagtgcc acattcactg gcattcacaa taacatgata ttcatcacgg
       61 agtgactatg ttacagatag tcggcgcgct gatcctgctg atcgcaggat ttgccattct
      121 tcgccttttg ttcagagcat taaccagcac ggcgtctgcg ctggcaggat tcgtattgct
      181 gtgtctgttc ggcccggctt tactggctgg ctatatcact gaacgcataa cccggttatt
      241 ccatattcgc tggctggcag gcgtatttct gacgattgcc ggaatgatca tcagcttcat
      301 gtggggactt gatggtaaac atatcgcact ggaggctcat acttttgact ctgtaaaatt
      361 tattctgacc accgctctcg ccgctggtct gctggctctt cccgtgcaga taagaagcat
      421 tcagcagaac gggctcacac ctgaagatat cagcaaggaa attaacgggt attactgctg
      481 tttttatact gcttttttcc ttatggcgtg ttctgcatac gcaccattga tcgcattgca
      541 gttcgatatt tcaccctcac tgatgtggtg gggcgggttg ttgtactggc tggctgcatt
      601 agtgacgctg ctatgggcgg ccagccagat ccaggcgctg aaaagactga ccagtgccat
      661 ccgccagaca ctggaagaac aaccggtgct caacagtaaa tcgtggctga gcagtttgca
      721 aaacgattac agccttcctg aaacgctgac ggagcgcatc tggctgacac tcatttcaca
      781 acggatttcc cggggagaac tgagggaatt tgaactggca gacggaaact ggctactgga
      841 caatgcctgg tatgaaagaa acatggcggg tttcaacgaa aagctgagag agagcctgtc
      901 atttacccct gatgaactga aaaccctctt ccggaaccgc ctgaatttat caccggaagc
      961 gaatgacgat tttctcgatc gttgcctgga cggcggtgac tggtacccct tttcagaagg
     1021 ccgccgtttt gtatcattcc accacgtgga tgagcttcgt atctgtgcct cctgcgggct
     1081 gacagaagta catcatgccc cggaaaatca taagccggat ccggaatggt actgctcctc
     1141 tctttgtcgc gaaacagaaa cactgtgtca ggacatttat gaacgttctt acaccggttt
     1201 tatttccgat gcaacggcga atggtctgat tctcatgaaa ctgccggaaa cctggagtac
     1261 aaatgagaaa atgtttgctt ccggagggca gggacatggg tttgccgctg aacggggaaa
     1321 ccatattgtc gacagagtcc gtctgaaaaa cgcacggatc ctcggtgata ataatgccag
     1381 aaatggagca gacagactgg tcagcggaac agaaatccag acgaaatatt gttcaactgc
     1441 agcccgtagc gtcggtgcgg cattcgacgg acaaaacgga cagtatcgtt acatgggaaa
     1501 tcatggtccc atgcaactgg aagtcccccg tgatcagtat gccggcgctg tggaaaccat
     1561 gaagaataag atccgcgaag gtaaagtacc cggtgtaacc gatcccgcag aagcgtcccg
     1621 gctgattcgt cggggacatc tgacttatac ccaggcccgt aatatcaccc ggttcgggac
     1681 catcgaatcg gtcacttatg atattgccga ggggtcggtt gtcagtctgg cggccggagg
     1741 gatcagtttt gccctgacgg catcggtctt ctggctcagc accggcgatc gcgatgctgc
     1801 cctgcagaca gctgctgtcc aggcaggaaa aaccttcacc cgcacactgg ctgtctacgt
     1861 cacaacccag caacttcacc ggctcactgt tgttcagggt atgctgaagc atattgattt
     1921 ttcgacggcc agcccgactg tccggcaggc gcttcagaag gggaccggtg caggaaatat
     1981 cagtgccctg aacaaagtga tgaagggtac gctggtgaca tctctggcac tggtcgctgt
     2041 cacaaccggc cctgacatga tcaaaatgtt gcggggacgg atctccggtg cgcagttcat
     2101 caggaatctt gccgtggcat cttccggtgt ggcaggtggt gctgtcgggt cagtggcggg
     2161 cgggatattg ttcagtccac tgggaccatt tggtgcactg acaggacgtg tggttggcgg
     2221 tgttctgggg ggaatgattg cctccgctgt atcaggaaaa attgccggag cgctggttga
     2281 agaagatcgc gtcaaaattc tggcaatgat tcaggagcag gtgacatggc ttgccggcag
     2341 tttcctgctg accggacatg agattgaaaa tctgaacgcg aatctggccc gtgttatcga
     2401 tcagaatgct ctggagatca ttttcgccgc cggtatacaa caacgggccg cgaccaatat
     2461 gttaatcaaa ccactggtgg tcagtatcat caggcaacgc cccgtcatgg aatatgatgc
     2521 atcccatctc ggcaatatgg ttaaccgact ggaagaagca ttacccccgg agttaccggc
     2581 ataacggcta ttctgcgcct tttacgtttt atcacctgat ttgtcagtgt catcgtcatg
     2641 actcagagag gtaaatacca tgattcacct gttcaaaacc ggcatgatta ccgccttcat
     2701 tctggggtta acgtggtctg ccccactccg ggcacaggat caacgttaca tcagtatacg
     2761 caatacagat acgatatggc tcccgggaaa tatttgtgcc taccagttcc ggctggataa
     2821 tggcggaaac gatgaaggat ttggccccct caccatcact ctgcaactca aagacaaata
     2881 tggtcagacg ctggtgacca gaaaaatgga aacggaagcc tttggtgaca gtaatgccac
     2941 gcgaaccaca gacgcatttc tggaaacgga gtgcgtggaa aatgtcgcca caaccgaaat
     3001 cattaaagca actgaagaaa gtaacggcca tcgtgtcagt ctgccgttat cggttttcga
     3061 tccccaggac taccatccac tgctgattac cgtttccgga aaaaatgtta actgatccct
     3121 gaagtcagtt tcatgcttca gcctctgcca tacgttgtgg cagaggctat ttatcaggag
     3181 aaaccagata tgccgggtta cacacagagt atgtcctggc tgaaggatct ttctcatacg
     3241 ggcaggcggt tgctgtcata accgcttacc gcaatgtgtt tatccaggat gacccgggaa
     3301 tgcatttccg tcgggttatc cgcaacgcgg aaggtcagcg acgctgacgc tgccggaact
     3361 ctgaagctga tgccggaaaa cagcttaatg cgtggctcgc cagtggaggg cttctcagac
     3421 aataaacctc attaatttca cccatcaggc cgcgtcttct ccgggagacg cggctttttt
     3481 catttattac accagtaaat cttaaccacc gataaggagc aaagtatgcg attagtcagt
     3541 cgttttggtt atgctgcaaa ccagatacgc cgtgaccgtc cgctgacaca tgaagagctg
     3601 atgcaccatg tgccggggat tttcggggaa gagaaacaca cgtcccgaag ccagaactat
     3661 acgtacatcc ccaccatcac cgtactggaa agtctgcagc gggaaggctt ccagcccttt
     3721 ttcgcctgcc agacccgtgt gcgcgacccg ggccgccggg gatacacaaa acacatgctg
     3781 cgtctgcggc gggccggaga gataaacgga gaacatgtcc ctgaaattat tctgctcaac
     3841 tcccatgacg gtacctccag ctaccagatg ctgccgggtt acttcaggtt cgtctgccag
     3901 aacggatgcg tctgtggtca gtctctgggg gaagtgcgtg ttccgcaccg gggaaatgtg
     3961 gtggacagag tcattgaagg ggcttacgag gtggtgggcg tgtttgaccg gatagaggag
     4021 aagcgtgatg ccatgcagtc gctggtcctg ccgccaccgg cacgccaggc gctggcacag
     4081 gcggcactga cttaccgtta tggtgacgaa catcagcccg tcactaccgc cgacattctg
     4141 acaccgcgac gccgggagga ttacggtaag gacctgtgga gtgcttatca gaccatccag
     4201 gagaatatgc tgaaaggcgg gatttccggt cgcagtgcaa aaggaaaacg tatccacacc
     4261 cgtgccattc acagcatcga caccgatatt aagctcaacc gcgcattgtg ggtgatggca
     4321 gaaacgctgc tggagagcat gcgctgatga aacatatccc tgaaggcgtg attcaggcac
     4381 cagaatcacc cgacgccccg gaagcgctgc cggggtgtcg ttccatttct gatgatgacc
     4441 tgtgcgcctg gtgtacccgt ctgctttacc gtcccggaga actcagtctg tgcagactga
     4501 gtacaggcaa tggcatctgg ccatccgtct gtgaccggaa cggttacgcg tacggctgtc
     4561 cggaatttca gccgaatatc attcatccct gatattgccg ggtctcacgg tcagtgatga
     4621 acgtgatgtt gtcagccccc ggcctttatt tcctctcatt tattcacatt acaaggattt
     4681 cacacatgaa aacattgtct cagaacacca cctcttcagc ctgtgcaccg gagactggcc
     4741 tgcaacaact ggttgccacc atagtccctg atgaacagcg catcagcttc tggccgcagc
     4801 attttagcct cattccacag tgggtgaccc tggagccccg tgtcttcggc tggatggacc
     4861 gtctgtgcga agactactgc gggggcatct ggaatctgta caccctgaac aacggcgggg
     4921 catttatggc ccctgaaccg gatgacgatg atgacgaaac atgggtgctg tttaacgcca
     4981 tgaacggcaa ccgcgctgaa atgagcccgg aagccgccgg cattgctgcc tgtctgatga
     5041 cgtacagtca tcatgcctgt cgcacggagt gttatgccat gacggttcat tattaccgtc
     5101 tgcgggacta cgccctgcag catccggaat gcagcgccat tatgcgcatc attgactgaa
     5161 agggggccgg aacaatgcaa caactttcct ttctgcccgg cgcgatgacg cccggcgagc
     5221 gcagcctcat tcagcgggcc ctgaaaaccc tggaccgcca tcttcatgaa cccggcgtgg
     5281 ccttcacctc cacccgtgcg gcccgggaat ggctgattct gaacatggct ggactggagc
     5341 gtgaagaatt ccgggtgctg tatctgaaca accagaataa gctgattgcc ggtgaaaccc
     5401 tcttcaccgg caccatcaac cgtacggaag ttcatccccg ggaagtgatt aaacgcgccc
     5461 tgtaccacaa tgctgctgcc gtggtgctgg cgcacaatca cccgtccggt gaagtcacac
     5521 ccagcaaggc
//
LOCUS       DAZGVQ010000251         5373 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000251 DAZGVQ010000000
VERSION     DAZGVQ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.254"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            8..283
                     /gene="yafQ"
                     /locus_tag="ACHLAG_004985"
     CDS             8..283
                     /gene="yafQ"
                     /locus_tag="ACHLAG_004985"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFP0658062.1"
                     /translation="MQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPA
                     VYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            439..1179
                     /gene="dpaA"
                     /locus_tag="ACHLAG_004986"
     CDS             439..1179
                     /gene="dpaA"
                     /locus_tag="ACHLAG_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFP0658063.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1150..1917)
                     /gene="yafJ"
                     /locus_tag="ACHLAG_004987"
     CDS             complement(1150..1917)
                     /gene="yafJ"
                     /locus_tag="ACHLAG_004987"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFP0658064.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2123..2701)
                     /gene="lpcA"
                     /locus_tag="ACHLAG_004988"
     CDS             complement(2123..2701)
                     /gene="lpcA"
                     /locus_tag="ACHLAG_004988"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFP0658065.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2941..>5373
                     /gene="fadE"
                     /locus_tag="ACHLAG_004989"
     CDS             2941..>5373
                     /gene="fadE"
                     /locus_tag="ACHLAG_004989"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFP0658066.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKV"
BASE COUNT         1273 a         1370 c         1453 g         1277 t
ORIGIN      
        1 aaatacgatt caaagggata ttgaatactc gggacaattt tcaaaggatg taaaacttgc
       61 acaaaagcgt cataaggata tgaataaatt gaaatatctt atgacgcttc ttatcaataa
      121 tgctttaccg cttccagctg tttataaaga ccacccgctg caaggttcat ggaaaggtta
      181 tcgcgatgct catgtcgaac cggactggat cctgatttac aaacttaccg ataaactttt
      241 acgatttgag agaactggaa ctcacgcggc gctctttggg taaagcacgc tgccgatgaa
      301 atttgtaaac cctgcgccga caggcttgcg taaaactctt ttggtgataa tgtgagcctt
      361 ccaaatccag actattccga tttgggatct attttgccgt cctggcgtgt gtaaccgttt
      421 tatcaaggaa taagcagtat gcgtaaaatc gcattaattc ttgcgatgtt gttgatcccg
      481 tgcgtttcgt ttgccggttt gctgggcagc agtagctcga ccacgccagt gagcaaagag
      541 tataagcagc agttgatggg atcccctgtc tacatccaga tcttcaagga agaacgtacg
      601 ctcgatctct acgtcaaaat gggcgagcaa tatcagctgc tcgacagtta taaaatctgt
      661 aaatattccg gcggcttagg gccaaaacag cgtcagggcg atttcaaaag cccggaaggg
      721 ttttatagcg tccagcgtaa tcagttaaaa ccagacagcc gttactacaa agcgattaat
      781 attggtttcc ccaatgccta tgaccgtgcg catggttatg aagggaaata cctgatgatc
      841 cacggcgatt gtgtttccat cggctgctac gcaatgacca atcagggtat tgatgagatc
      901 ttccagttcg ttactggtgc gctggtgttt ggtcagccga gcgtgcaggt gagtatttac
      961 ccgttccgca tgaccgacgc caatatgaag cgccataaat attccaactt taaggacttc
     1021 tgggagcaac tgaagccggg ttacgactac tttgagcaaa cccgtaagcc accgaccgtt
     1081 tctgtggtca atggtcgtta cgtggtcagc aagccgttaa gccacgaagt tgtgcagcca
     1141 caactggcat caaactacac gctccccgag gcaaaataag cgccattcgc ctggcataat
     1201 cttttgccag gtttcattgc ccgtcagcgg ctgtgttgca atcaccgtga ccacatcatt
     1261 cggtgtggtc tgcgagctga agtcgatttc cacatcctga tccagcagcg ttgccacgcc
     1321 aaacggcgcg cggcgggtga tccagtgtaa attagtcgag caatacgcca tcacatagcg
     1381 cccgtcagaa agcagcatgt tgaaaacgcc cttctgccgc agttcatccg ccagtgaggc
     1441 gatatattta aacactgccg ccatgttgcc cggtgtgcgc gggtaacgct gcgttaattt
     1501 atgcaggagc cagcagaagg ctttttcgct atcggtttca ccgaccgggc ggaagttgcc
     1561 ggtttccagt gatttgtagc ccgtcagttg tccgttatgg gcataagtcc agttacgccc
     1621 ccataactcg cgggtaaatg ggtgagtatt ttccagcgcc acctcgcccc gattagcctg
     1681 gcgaatatga gccaccaccg aacaggattt tatcgggtag tcctggacaa gtttggcgat
     1741 gggggaatta aagctgggtt gtggatcttt aaatgtgcga cagcctttac cttcgtaaaa
     1801 ggtaatgccc cagccatctt tatgtggccc ggttccacca ccacgctgta caagtccggt
     1861 gaaactaaag cagatatcgg tagggacgtt ggcgctcatc ccgagcaatt cgcacatggg
     1921 taacctccac aacagcggga gcgaatggcc cccggcgaag attgatttaa gaattttcag
     1981 gtcggatgcg gcgtaaacga attattcggc ctacggttcg ggtacagatt tgtaggcatg
     2041 ataagacgcg tcagcatcgc atcaggcatc agcgcacaag tgccggatgc ggcgtaaacg
     2101 ccttatccag cctacgccag acttacttaa ccatctcttt ttcaattaac tggatcagga
     2161 tatggatcac tttaatgtga atctcctgaa tgcggtcggc ataaccaaag tgcggtacgc
     2221 gaatttcgat atccgccgtg ccagccattt tgccgccgtc tttaccggtc agggtgatca
     2281 ctttcatccc cttctcacgc gccgctgcga tcgctttgat cacgtttgca gagttaccgg
     2341 aggtggagat ccccagcagt acatcgcctt cgcgacctac cgcttcaacg taacgggaga
     2401 aaatatcatt gaaaccgaaa tcgttaccga cgcatgaaat atgactaacg tcagaaatag
     2461 caatcgccgg gtagcccgga cggttttcac ggtagcgacc ggtcaactct tcggcaaagt
     2521 gcatagcgtc gcaatgggaa ccgccgttgc cgcaggaaag cactttgcca ccggctttaa
     2581 agctgtctgc taacaggacc gccgcgcgct gaatggcgtg aatattggcg tcatctttta
     2641 aaaagttagc cagcgtttcc gccgcttcgt tcagttcgtt acgaataaga tcctggtaca
     2701 tgaggatatc cttcagcata aatgtaatag acaaaatgca gtgtaccgga taccgccaaa
     2761 agcgagaagt acgggcaggt gctatgacca ggactttttg acctgaagtg cggataaaaa
     2821 cagcaacaat gtgagctttg ttgtaattat attgtaaaca cattgctaaa tgtttttaca
     2881 tccactacaa ccatatcatc acaagtggtc agacctccta caagtaaggg gcttttcgtt
     2941 atgatgattt tgagtattct cgctacggtt gtcctgctcg gcgcgttgtt ctatcaccgc
     3001 gtgagcttat ttatcagcag tctgattttg ctcgcctgga cagccgccct cggcgttgct
     3061 ggtctgtggt cggcgtgggt actggtgcct ctggccatta tcctcgtgcc atttaacttt
     3121 gcgcctatgc gtaagtcgat gatttccgcg ccggtatttc gcggtttccg taaggtgatg
     3181 ccgccgatgt cgcgcactga gaaagaagcg attgatgcgg gcaccacctg gtgggagggc
     3241 gacttgttcc agggcaagcc ggactggaaa aagctgcata actatccgca gccgcgcctg
     3301 accgcagaag agcaagcgtt tctcgacggt ccggtagaag aagcctgccg gatggcgaat
     3361 gatttccaga tcacccatga gctggctgat ttgccgccgg agttgtgggc gtaccttaaa
     3421 gagcatcgtt tcttcgcgat gatcatcaaa aaagagtacg gcgggctgga gttctcggct
     3481 tatgcccagt ctcgcgtgct gcaaaaactc tccggcgtga gcgggatcct ggcgattacc
     3541 gtcggcgtgc cgaactcatt aggcccgggc gagctgctgc aacattacgg cactgacgag
     3601 cagaaaaatc actatctgcc gcgtctggcg cgtggtcagg agatcccttg ctttgcactg
     3661 accagcccgg aagcgggttc cgatgcaggc gctatcccgg ataccgggat tgtctgcatg
     3721 ggcgaatggc agggccagca ggtgctgggg atgcgtctga cctggaacaa acgctacatt
     3781 acgctggcac cgattgcgac cgtgcttggg ctggcgttta aactctccga cccggagaaa
     3841 ttgctgggtg gcgcagaaga tttaggtatt acctgtgcgc tgatcccaac caccacgccg
     3901 ggcgtggaaa ttggtcgtcg ccacttcccg ctgaacgtgc cgttccagaa tggaccgacg
     3961 cgcggtaaag atgtcttcgt accgatcgat tacattatcg gcgggccgaa aatggccggg
     4021 caaggctggc ggatgctggt ggagtgcctc tcggtaggcc gcggcatcac cctgccttcc
     4081 aactcaaccg gcggcgtgaa atcagtagcg ctggcaacgg gcgcgtatgc tcacattcgc
     4141 cgtcagttca aaatctctat cggtaagatg gaagggattg aagagccgct ggcgcgtatt
     4201 gccggtaatg cctacgtgat ggatgctgcg gcatcgctga ttacctacgg cattatgctc
     4261 ggcgaaaaac ctgccgtgct gtcggctatc gttaagtatc actgtaccca ccgcgggcag
     4321 cagtcgatta ttgatgcgat ggatatcact ggtggcaaag gcattatgct cgggcagagc
     4381 aacttcctgg cgcgtgctta ccagggcgca ccgattgcca tcaccgttga aggggccaac
     4441 attctgaccc gcagcatgat gatcttcgga caaggagcga ttcgttgcca tccgtacgtg
     4501 ctggaagaga tggaagcagc gaagaacaat gacgtcaacg cgttcgataa actgttgttc
     4561 aaacatatcg gtcacgtcgg tagcaacaaa gttcgcagct tctggctggg cctgacgcgc
     4621 ggtttaacca gcagcacgcc aaccggcgat gccactaaac gctactatca gcacctgaac
     4681 cgcctgagcg ccaacctcgc cctgctttct gatgtctcga tggctgtgct gggcggcagc
     4741 ctgaaacgtc gtgagcgcat ctcggcacgt ctgggggata ttttaagcca gctttacctc
     4801 gcctctgccg ttctgaagcg ttatgacgac gaaggccgta atgaagccga cctgccgctg
     4861 gtgcactggg gcgtacaaga tgcgctgtat caggctgaac aggcgatgga tgatttactg
     4921 caaaacttcc cgaaccgcgt ggttgccggg ctgctgaatg tggtgatctt cccgaccgga
     4981 cgtcattatc tggcaccttc tgacaagctg gatcataaag tggcgaagat tttacaagtg
     5041 ccgaacgcca cccgttcccg cattggtcgc ggtcagtacc tgacgccgag cgagcataat
     5101 ccggttggct tgctggaaga ggcgctggtg gatgtgattg ccgccgaccc aattcatcag
     5161 cggatctgta aagagctggg taaaaacctg ccgtttaccc gtctggatga actggcgcac
     5221 aacgcgctgg cgaaggggct gattgataaa gatgaagccg ctattctggt gaaagctgaa
     5281 gaaagccgtc tgcgcagtat taacgttgat gactttgatc cggaagagct ggcgacgaag
     5341 ccggtaaagt tgccggagaa agtgcggaaa gtt
//
LOCUS       DAZGVQ010000252         5355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000252 DAZGVQ010000000
VERSION     DAZGVQ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.255"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1121)
                     /gene="mobQ"
                     /locus_tag="ACHLAG_004990"
     CDS             complement(<1..1121)
                     /gene="mobQ"
                     /locus_tag="ACHLAG_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="HFP0658067.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLTHDYTR
                     KRGVDDAVILTPENAPSWCADRSVLWNAVEKAEQRRDSQLAREIELAIPREISRDAAR
                     ETVLAFVRENFVSQGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRAL
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKSACAMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTQLALRKVQETQRKRELSDAARRAAEALNLTIPAANASA
                     DTLREFIATLPQECGNAWEMTPEFVAMNGKANDIKREGNALVKERDILEKEMTGLRKA
                     RPVASILSELPLMTWAEPEYRKRQLRFWKLGKQIESLSRT"
     gene            1300..1578
                     /locus_tag="ACHLAG_004991"
     CDS             1300..1578
                     /locus_tag="ACHLAG_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HFP0658068.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRIQPLINELEKLPRPPVKPQKND"
     gene            2108..2803
                     /locus_tag="ACHLAG_004992"
     CDS             2108..2803
                     /locus_tag="ACHLAG_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP0658069.1"
                     /translation="MTDLIAYKSNALVEASYKLTLQEQRLLLLCISRLNSSNATPESQ
                     KTMMITAEDFFNSFPDMGRNHAEEKLQEAIDRLWDRSIILKDDEKREEMRWLQYRAHY
                     LKGEAKVQITFADAVMPYLTQLKGQFTKVVIKNISRLSRSYSIRIYEILQQFRSTGER
                     VIALDDFRSMLQIDKKYKDFKTLNRSLIKPSIAELNQKSDLAVTVEPIKKGRTVVALR
                     FLFKEDKQIKAPI"
     gene            3174..4289
                     /locus_tag="ACHLAG_004993"
     CDS             3174..4289
                     /locus_tag="ACHLAG_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP0658070.1"
                     /translation="MTKIYIYLVLAASLSALSTDMIVPALPELQKAFNADYSLIQLSI
                     SGFLIVFAFSQLISGTIGDHFGKVRVMTICLIIFLIGSIVCFMANNLNMLILGRILQA
                     IGAGAGPVISKAIAKESFPPLKLKRALSDISSTSATIPLIAPLAGALILDYYNWNVIF
                     IVMGSFSILTMLLSPKEANKQEKKTHQDDIKFVTKDFICGTILVSLMLSSLFCYISIS
                     PALFMNDYGLNTFNYSVVFSLSVLFFIIGNQLSKIEKISDPWVLFPLNAISVIPFLIL
                     NDNFVICVIGAMIFNLTLGAYYPIANFISLQINGSRTGLAASITGFTQTVSAGVISFL
                     SVKISDNGVSLGTTLSISCLILALLSIFVTIIGDNKK"
     gene            complement(4556..>5355)
                     /locus_tag="ACHLAG_004994"
     CDS             complement(4556..>5355)
                     /locus_tag="ACHLAG_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFP0658071.1"
                     /translation="SNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGAVMVSDIREGM
                     PESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNFYFSDDRRPVA
                     SPLPSAHRVDITADAVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVGFVRREGGFCP
                     VLVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQFRLKGHHDRLL
                     AAVHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
BASE COUNT         1438 a         1210 c         1239 g         1468 t
ORIGIN      
        1 gtgcggctga gagattcaat ctgtttcccg agtttccaga aacggagttg tcttttgcgg
       61 tattccggtt cagcccatgt catcagggga agctctgaca ggatggacgc taccggacgg
      121 gcttttctga gtccggtcat ctctttttcg agaatatccc gctctttcac cagcgcattc
      181 ccctcacgtt tgatgtcgtt tgccttcccg ttcatcgcca cgaactcagg ggtcatctcc
      241 catgcgttcc cgcattcctg cggcagtgtg gcaatgaatt cccgcagggt atccgctgag
      301 gcattcgcgg cgggaatggt cagattaagg gcttccgctg cgcgacgtgc ggcatcgctg
      361 agttcccgct tacgctgcgt ttcctgaacc ttcctgagcg ccagctgcgt gcgggaaacc
      421 tgtatttcca ggttaaggct gttaatcagg cggttctgct caccgcgttc tgtttccatg
      481 ccccgttttt ccatcgcaca ggcagacttt ccgaggtgaa ggcccggcgc tttctccagt
      541 ccctgacgct cgtatgaacg atggtctatc tcttcctggt agccggcgtt agcaagcgct
      601 ctgttcgcat ggtcagccca tgatgcgcgc cacgtctcgg cgagtgcccg gtcgttccag
      661 tccctgacct ttcctgcgaa tcccgtttcc ccgacagctc ttgtggtcag catgatgtgc
      721 gcatggggat tagtccggtc catgtgatgg aacgccacat cggcaatcat gccctggctg
      781 acaaagtttt ccctgacaaa agcgagaacg gtttcccgtg cggcatcgcg ggaaatctct
      841 cggggaatgg caagttcaat ctcccttgcc agctgggagt cccggcgctg ttcggctttc
      901 tcgaccgcat tccagagaac ggaacggtca gcacaccatg acggtgcatt ctcaggggtg
      961 agaatgaccg catcatcaac gccgcgttta cgggtgtaat cgtgggttaa tccggtacgg
     1021 tcatcgggta tgacagagcc ggaacggtag gcagcagaag caacggcact gtagccgttt
     1081 tttcgcgaaa tgattttcat gctgagatga taaatcgcca tttctgccgt aatgcctttt
     1141 tgccgttttc tgtcagcccc gcagggcgca cacaatcgaa acggagtgga gattgtataa
     1201 gtgcgcactt cgtgatgtgc cgtgcattat gccagttcag ttttgcaact accagtgacg
     1261 cgctaaaatg cgatttttca ccgcgcacgg agtcacaaaa tgagcagaac actcgaacag
     1321 aagattgccg aagctgaggc cagattacag cgcctgaagg cgaagagccg gagcctggac
     1381 acggcgcaaa aagtcattgt gggggctgcc atgctggcga gggtacggag gccggaagaa
     1441 gcgcaactgc gtgcgttcct gctccagttt ctgcgaaagg aagtcacaag acaggcggat
     1501 gtgaaccgga tacaaccgct gattaacgag ctggagaagc tacccagacc acctgtgaaa
     1561 ccgcaaaaga acgattgatc gccttccgct cgccgcagcc ccagcgaccg agcgttagcg
     1621 agtaagtggg cgaggaagcg gcagaagtgc cgttgctgaa tgtggcacgt acgcactgtt
     1681 ttcagaaagg tgatcctctc tgaacctgca tgaatactgg atttccacag gtttgcatgt
     1741 tgtttttgca gaacgcggca aactgatttt ttataacatg ttgtttttta gtgttttttt
     1801 gttttcccgc tttgagtttt caacaggcac gccgttaagt ttttagatct tgtttttata
     1861 tacttgtttt tatacttgtt ttagggttga gatttctttt ttaaatcaat atgttgcaat
     1921 gacataggtg aacattttgg gggtataggt gaacattttg ggggtggtta ggtgaacatt
     1981 ttgggggtat aggtgaacat tttgggggtg gttatccaca gaaaaaggtg aatcaaaaaa
     2041 aaaatattgc acttatggta aaggttaatt actttttttt ttgttaacct atcaattggg
     2101 gaacgtaatg acggatttga ttgcttacaa atcgaacgca ttggttgaag ctagctacaa
     2161 actaaccttg caggagcaac gcttgctttt gctttgtatt agccgattga actccagcaa
     2221 tgcgacgccg gaatcgcaga aaactatgat gattacggca gaggattttt ttaattcatt
     2281 tcctgacatg ggtcggaatc acgccgaaga aaaattacaa gaagcgatag atcgtttatg
     2341 ggataggtcg attattctca aagatgacga gaagcgagaa gaaatgcgtt ggctccagta
     2401 tcgagcacac tacctcaagg gcgaagctaa agttcagata accttcgcag atgctgtaat
     2461 gccctatctg acacaattaa aaggtcaatt cacgaaagta gtaattaaaa atatatcaag
     2521 attatcaagg agctacagta ttcggatcta cgaaattctc cagcaattcc gtagcacagg
     2581 agagagggtt attgctcttg atgattttcg cagcatgcta caaattgata aaaagtataa
     2641 ggatttcaaa acattaaata gatctttgat aaaaccatcg atagcggaat tgaatcaaaa
     2701 aagcgatctg gctgtgacgg tagagccaat caagaagggg cgaacggtgg tcgctctgcg
     2761 ttttctgttc aaggaggaca aacagatcaa agctcctatc taaatgcact taaaacgctc
     2821 tcagacgcgt tctgacgcaa aaaacgcaaa aagacaagga aacaccaggc caaaccgaaa
     2881 aaacgcgtca gaatcgtttt taggtagatg cataactatg cacgaaagct atgcgcgatc
     2941 aatttcgacc gctccgaagg gcgaacaggt caaaatgaca agttttctcc ctccagatgg
     3001 aaggttcagc acggggattt tttacctcct tccatcaacc tgaatatgca tctggaagta
     3061 cccattagtt tctgctcaaa cactatcgca gtgctatctc aaaatataaa atataccgat
     3121 ggtgtaagta ttttaaatat gataaataag aatattttta tcaggggtta attatgacaa
     3181 agatatatat ttaccttgtt cttgctgcat cattatcggc attatcaact gacatgattg
     3241 tgcctgcgtt accagaacta cagaaagcat ttaatgctga ctattcatta attcaactaa
     3301 gtattagtgg atttcttatt gtatttgcat ttagtcaact gatatcaggt actataggtg
     3361 atcattttgg aaaggtacgc gtgatgacta tatgcttaat tatatttctt attggcagca
     3421 ttgtatgctt tatggccaat aatttaaata tgcttatttt gggtagaatt ctgcaggcaa
     3481 taggtgcagg tgctggacct gtaatatcaa aagcaattgc taaagagtct tttccaccac
     3541 taaagctaaa aagagcactt tctgacatat catcgacaag tgcgacaata ccattaatag
     3601 cacctctggc tggagcatta atacttgatt attataactg gaatgtaata tttattgtaa
     3661 tggggtcatt tagcattcta accatgctcc tgtcacctaa agaggctaat aaacaggaga
     3721 aaaaaacaca tcaagacgat ataaaatttg tgactaaaga tttcatctgc gggacaatac
     3781 tcgtttcact aatgctaagc tcactatttt gctacatatc aatatcaccc gcactattta
     3841 tgaatgacta tggattaaat acatttaact actctgttgt tttttcccta tcagttttat
     3901 ttttcatcat tggaaaccaa ttatcaaaaa tcgaaaaaat atcagatcca tgggttttat
     3961 ttcctctaaa tgcaatatca gtcataccct ttctcatatt aaatgataat tttgtaatat
     4021 gtgttattgg agcaatgatt ttcaatttaa ctcttggcgc atactatcca atagctaatt
     4081 ttatctcatt acagataaac ggttcacgaa ctggcttggc cgcatcaatt acaggattta
     4141 ctcaaacagt aagcgcaggg gtaatcagtt tcctttcagt aaaaataagc gataatggag
     4201 tttctttggg aactacttta agtatcagct gtcttattct tgcattattg tcaatattcg
     4261 taactattat aggggataat aaaaaatgaa ttggcaataa agaaaaagct atcacaatat
     4321 gcaaaaaaat gcatgatcgt ggttttgcta caaccgttgc tatgtatcct acagtggaag
     4381 agggaaaggc gattctacgt tgtgcaatat ctgctattca taccagagaa aatatagata
     4441 gcctacataa aaactttatg gaatgtatta acgaaccggg ttagtaatat catggcctct
     4501 ttatatagag gccctctatt tcatttttta tggcccgtgg cgcagccctc gaatatcaca
     4561 tactcatgcc ccggaagtgc ccggggcggc gttgttgttg cctgtgctgt tcgtcccgct
     4621 tccactcatg ccaggtctga ttaaaaccga gttgacggaa gagataatcc ggcatctctt
     4681 ccggtttgtg gacagcagcc aaaagcctgt catgatggcc tttgagccgg aactgcatca
     4741 ggtgcggttc cggtgggtac ggagtatcct catcatggtt tatagcttct ccgtaacctg
     4801 ccaggcatac cccgttctcc gtgatggtgc tgactatcag atgactgtaa tccggcgacg
     4861 gaagcacaag aacggggcaa aaccctcctt cccggcgaac aaacccgaca agcatttcct
     4921 ccggcccttt gcataacaac agattcagcg tcctgctgtt tccctctctc gaaaatacaa
     4981 caggcatgag ttgttccggt acagcatcag cggtgatatc cacccggtgc gctgacggca
     5041 gcggagaggc tacgggcctg cggtcgtcgc tgaaataaaa attatccggc cagtgatgcg
     5101 gcagcgccgg aaaaccgtaa gagggccacc cctggcgcat ctgttccagt acatgccgga
     5161 ccgtcgcacc ggaatacatt ctcacacgct caggccgcgc catcgtctca tgagattctg
     5221 gcattccctc gcgtatatcc gagaccatca cagccccgcc tggcgacagg taaacattca
     5281 ccgggtcatg aacactctgg gggtaatgcg ccagctcatc accccatacc gtgaattctc
     5341 cggctctgtt actca
//
LOCUS       DAZGVQ010000253         5344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000253 DAZGVQ010000000
VERSION     DAZGVQ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.256"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..151
                     /locus_tag="ACHLAG_004995"
     CDS             <1..151
                     /locus_tag="ACHLAG_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFP0658072.1"
                     /translation="VALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKIIGGC
                     GPTAQ"
     gene            447..1784
                     /gene="frlA"
                     /locus_tag="ACHLAG_004996"
     CDS             447..1784
                     /gene="frlA"
                     /locus_tag="ACHLAG_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFP0658073.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLPGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            1805..2827
                     /gene="frlB"
                     /locus_tag="ACHLAG_004997"
     CDS             1805..2827
                     /gene="frlB"
                     /locus_tag="ACHLAG_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFP0658074.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2877..3707
                     /gene="frlC"
                     /locus_tag="ACHLAG_004998"
     CDS             2877..3707
                     /gene="frlC"
                     /locus_tag="ACHLAG_004998"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFP0658075.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYDGYCTVELVTMYMNEPRLYACQALERFRALLPEDER
                     "
     gene            3704..4489
                     /gene="frlD"
                     /locus_tag="ACHLAG_004999"
     CDS             3704..4489
                     /gene="frlD"
                     /locus_tag="ACHLAG_004999"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFP0658076.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFNFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            4589..5320
                     /gene="frlR"
                     /locus_tag="ACHLAG_005000"
                     /gene_synonym="yhfR"
     CDS             4589..5320
                     /gene="frlR"
                     /locus_tag="ACHLAG_005000"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFP0658077.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
BASE COUNT         1209 a         1434 c         1424 g         1277 t
ORIGIN      
        1 agttgcactg gtaggtgcag tactggctac gttaactgca tgtactggtc atattgaaaa
       61 ccgtgataag aactgctctt acgactacct gctgcacccg gcaatttcta tttctaaaat
      121 cattggcggt tgcggtccta ctgcacagta agtctctgac agaaatcggc taacaccggt
      181 ttaattgccc gacgccctct ggattttccg agggcgtatt tgtttctata tccttcttgc
      241 tccccacccg taaaaatatt ttcactttaa tttcaatttg ataaccacat tcattagcca
      301 cagcgcgatc ctatgcgaaa ttttgtgatc ttcctccgca ttacataaca tcatatgttg
      361 ttatattcat tatgcattgt catgttacct tttaaatgac tgcaaactct cccctacaac
      421 gtgttggaca agaaaaaggt atcgctatgg gaagccagga actccaacgc aagctcggat
      481 tttgggccgt tcttgcaatc gccgtcggga caaccgtcgg ctccggtatt tttgtatctg
      541 tgggtgaagt ggcaaaagcg gcgggcacgc cgtggctgac ggtgctcgca tttgtcatcg
      601 gcgggttaat tgtgatcccg caaatgtgcg tctatgcgga actatcaacc gcttatccgg
      661 aaaatggcgc agattatgtt tatctgaaaa atgccggaag ccgaccgctg gctttcctct
      721 ccggctgggc cagcttctgg gccaacgatg cgccgtcatt gtcgattatg gcgctggcga
      781 ttgtcagcaa tcttggcttt ttaacgccta tcgatccgtt gcccggtaaa tttatcgccg
      841 ccggattaat tatcgccttt atgttgctac acctgcgctc cgttgaaggc ggcgcagcgt
      901 ttcagacgct aattaccatc gccaaaatta tcccgttcac tatcgtcatt ggccttggga
      961 tcttctggtt taaagcggag aattttgccg cccctaccac cactgcgatt ggcgcaacgg
     1021 gcagctttat ggcgctgctg gcggggatct ctgccaccag ttggtcgtat accggcatgg
     1081 cctctatctg ttatatgacc ggcgaaatta aaaaccccgg aaaaaccatg ccacgagcgc
     1141 tgattggttc ctgtctgctg gttctggtgc tctacaccct gctggcgctg gtgatttccg
     1201 gcctgatgcc cttcgacaaa ctcgccaatt ctgaaacgcc gatttccgac gccctgacct
     1261 ggatccccgc actcggcagc accgctggga tctttgttgc catcacggcg atgatcgtca
     1321 ttcttggttc gctttccagc tgcgtgatgt accagccgcg gctggaatac gcgatggcga
     1381 aagacaacct gttctttaaa tgcttcggcc atgtgcatcc gaaatacaac acgccggatg
     1441 tctccatcat cctgcaaggg gcgctgggga tcttcttcat cttcgtttcc gatctcacca
     1501 gcctgctggg ttatttcacc ctggtgatgt gtttcaaaaa taccctcacc ttcggctcca
     1561 tcatctggtg tcgtaaacgc gacgattaca aaccgctgtg gcgtactccg gctttcgggc
     1621 tgatgaccac cctcgccatt gcgtcaagcc tcattctggt cgcctcaacc tttgtctggg
     1681 caccgattcc cggccttatc tgcgccgtca tcgttattgc tactggtctg cctgcttacg
     1741 ccttctgggc gaagcgtagc cgccagctca acgctttgtc gtaatgttat ctggagaaaa
     1801 taaaatgttg gatattgata aaagcaccgt ggactttctg gtcaccgaaa atatggttca
     1861 ggaagtggaa aaagttctca gccatgacgt tccgctggtg cacgccatcg tggaagagat
     1921 ggtgaagcgc gacattgatc gtatttattt cgttgcctgc ggctcgccac tcaacgcggc
     1981 gcaaacggcg aaacatctgg cggatcgctt ttccgatctt caggtctacg ccatttccgg
     2041 ctgggagttc tgcgataaca ccccgtatcg cctcgacgat cgttgcgcgg tgattggcgt
     2101 ttctgactac ggtaaaacgg aagaggtaat caaagcgctg gagctgggcc gggcctgcgg
     2161 cgcactcact gcggcgttca ccaaacgcgc ggacagcccg attacctcgg cggcggaatt
     2221 tagcattgat tatcaggccg actgtatctg ggaaattcac ctgctgctct gctacagcgt
     2281 ggtgctggag atgatcaccc gcctcgcgcc gaacgcggaa atcggcaaga tcaaaaacga
     2341 tctcaagcag ttgccgaatg cgctcggtca tctggtacgc acctgggaag aaaaaggccg
     2401 ccagcttggt gaactggcca gccagtggcc gatgatttat accgttgctg cgggtccgct
     2461 gcgtccgctg ggttacaaag aaggcattgt aacgctgatg gaatttacct ggacgcacgg
     2521 ctgcgtgatt gagagcggag agttccgcca tggcccgctg gagattgtcg aaccgggcgt
     2581 tccgttcctg ttcctgctcg gcaatgatga aagtcgccac accaccgaac gcgccattaa
     2641 ctttgttaaa cagcgtactg acaacgtgat cgtcatcgat tacgccgaaa tttcgcaagg
     2701 gctgcacccg tggctggcac cgttcctgat gttcgtgcca atggagtggc tctgctacta
     2761 cctgtctatt tacaaagatc acaacccgga tgaacgccgc tattacggtg gtctggtgga
     2821 atattaatcc ctctcccggc ccggcaacgg gccgaattta cgcaaggagt tacccgatga
     2881 aaacaggtat gtttacctgc ggccaccagc ggctgcctat tgaacacgca tttcgtgatg
     2941 caagcgagct gggttacgac ggcatcgaaa tttggggcgg tcgcccgcac gcgttcgcgc
     3001 cggacttaaa agcgggcggc atcaaacaaa tcaaggcgct ggcgcagacg tatcagatgc
     3061 cgattatcgg ctatacgcca gaaaccaacg gctatccgta taacatgatg ctgggcgatg
     3121 aacatatgcg tcgcgaaagc ctcgacatga tcaagctggc gatggatatg gcaaaagaga
     3181 tgaacgcggg ttatacgctg atttccgcgg cccacgcggg ctatctcacg ccacctaatg
     3241 ttatctgggg acggctggca gagaacctaa gcgaactgtg tgagtacgcg gaaaacatcg
     3301 gcatggatct gatcctcgaa ccgttaacgc cgtatgaatc gaacgtcgtg tgtaatgcta
     3361 atgatgtgct tcatgcgctg gcgctggtgc cttcgccgcg cctgttcagc atggtcgaca
     3421 tttgcgcgcc gtatgtccag gcggaaccgg tgatgagtta tttcgacaaa ctgggtgata
     3481 aattgcgtca tctgcatatt gtcgacagcg acggggccag tgacacacat tacattcccg
     3541 gcgaaggcaa aatgccgctg cgggagctga tgcgcgatat tatcgaccgg ggctatgacg
     3601 gttactgcac ggtggaactg gtgacgatgt atatgaacga gcccagactc tatgcctgcc
     3661 aggcgctgga acgctttcgc gcgctgctgc cggaggatga gagatgaaaa ccctggcgac
     3721 aatcggcgat aactgcgtcg atatctaccc gcaactgaat aaagcgtttt ctggcggtaa
     3781 tgcggtcaat gtggcggtgt actgcactcg ctacggcata cagccgggat gcattacctg
     3841 ggtgggtgac gatgactacg gcacaaagct gaagcaggat ctcgcccgca tgggcgtcga
     3901 tatcagccat gtccatacga aacacggcgt taccgcacaa actcaggtgg aactgcacga
     3961 caatgatcgc gtttttggcg actacaccga aggcgtgatg gccgactttg ccctgagtga
     4021 agaggattac gcctggctgg cgcagtatga cattgtgcac gcggcaatct ggggacatgc
     4081 ggaagacgca ttcccacagc tgcacgctgc gggcaaactt accgctttca acttctccga
     4141 caagtgggac agcccgctct ggcagacact ggtgccgcat ctcgattttg cctttgcctc
     4201 cgcaccgcaa gaagacgaaa cgctgcgtct gaagatgaaa gcgattgttg cccgtggcgc
     4261 aggaacagtg attgtcacgc tgggtgaaaa cggcagcatt gcctgggatg gcgcgcagtt
     4321 ctggcgtcag gctcctgaac cggtgacggt tatcgacacc atgggtgccg gagattcgtt
     4381 cattgccgga ttcctttgcg gctggtctgc ggggatgaca ttaccgcagg cgatggcgca
     4441 gggaacggcg tgcgcggcga aaaccattca gtaccacggt gcctggtagg tataacgttg
     4501 gcgtgagcat cttcacgcca acgtgctgtt acttgccgga aaacgaccct ataatccgag
     4561 taattcattc tttatttcag ggtcgattat gtcagctacg gaccgctact ctcatcaact
     4621 cctctacgct accgtccgcc agcgactgct ggatgatatc gcgcaggggg tttaccaggc
     4681 cgggcaacag atccctaccg aaaacgagct ttgtacacaa tataacgtca gccgcattac
     4741 cattcgcaaa gccatcagcg acttagtggc agacggcgta ctgatccgct ggcagggaaa
     4801 aggcaccttt gtacaaagcc agaaagttga aaacgccctg cttactgtca gtggttttac
     4861 cgattttggc gtctcacaag gcaaggcgac gaaagagaaa gtgatcgaac aggaacgggt
     4921 cagcgccgcg ccgttttgcg aaaagctgaa catccccgga aacagcgaag tgttccatct
     4981 ctgccgggtg atgtatctcg ataaagagcc gctgtttatt gatagttcat ggatcccgct
     5041 gtcgcgttat cctgactttg atgagattta cgtcgaagga agctccacct atcagttatt
     5101 tcaggagcgt tttgacacgc gagtggtcag cgacaaaaag accatcgata tctttgccgc
     5161 cacccgcccg caggcaaaat ggctgaaatg cgaactgggc gaaccgttgt ttcgcatcag
     5221 caaaatcgcc tttgatcaga atgacaaacc ggtgcacgtc tccgaactct tctgccgcgc
     5281 caatcgcatc accttaacta ttgataataa aagacattaa ccgtaggccg gataagatgc
     5341 gcca
//
LOCUS       DAZGVQ010000254         5326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000254 DAZGVQ010000000
VERSION     DAZGVQ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.257"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            27..1229
                     /gene="fes"
                     /locus_tag="ACHLAG_005001"
     CDS             27..1229
                     /gene="fes"
                     /locus_tag="ACHLAG_005001"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFP0658078.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            1232..1450
                     /gene="ybdZ"
                     /locus_tag="ACHLAG_005002"
     CDS             1232..1450
                     /gene="ybdZ"
                     /locus_tag="ACHLAG_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFP0658079.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            1447..>5326
                     /gene="entF"
                     /locus_tag="ACHLAG_005003"
     CDS             1447..>5326
                     /gene="entF"
                     /locus_tag="ACHLAG_005003"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFP0658080.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINLAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLVQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGG
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISSEAFEKIGPIIRATLNR"
BASE COUNT         1147 a         1407 c         1565 g         1207 t
ORIGIN      
        1 ttgcacagta aacatggggt tatggtgtga cggcgttaaa agtaggaagt gagagctggt
       61 ggcagtcgaa acatggcccg gaatggcagc gtctgaatga cgaaatgttt gaggtcactt
      121 tctggtggcg tgatccccaa ggttctgaag aatactcgac gataaagcgc gtatggatct
      181 acatcactgg tgtgaccgat catcatcaga acagccagcc ccagtcgatg cagcgaattg
      241 caggtactga tgtctggcag tggacgacac aactcaatgc caactggcgc ggcagctact
      301 gctttattcc caccgaacgc gatgacattt tttctgcacc atcccccgat cgcctcgaat
      361 tacgtgaagg ctggcgaaaa ctattacccc aggcaatagc cgatccgctg aatccacaaa
      421 gctggaaagg cgggcgaggg cacgctgttt ctgcactcga aatgccgcaa gcgcctctgc
      481 aaccgggatg ggattgtccg caagcgccag aaatagcagc caaagaaatt atctggaaaa
      541 gtgaacggtt gaaaaattca cgtcgtgtat ggatttttac caccggcgat gcaacagcag
      601 aagaacgtcc gctggcagtt ttgctcgatg gcgaattttg ggcgcaaagt atgcccgtct
      661 ggcctgtgct gacttcgctg acccatcgtc agcaacttcc tcccgccgtg tatgtgttga
      721 tcgacgctat cgacaccacg caccgcgccc acgaactgcc gtgtaatgcg gatttctggc
      781 tggcagtaca gcaagagtta ttacccctgg tgaaagctat tgcccctttt agcgatcgcg
      841 ccgatcgcac cgtggtcgcc gggcagagtt ttggtgggct gtccgcgctg tatgccggac
      901 tgcactggcc tgaacgcttt ggctgtgtat taagccagtc aggatcgtac tggtggccgc
      961 atcggggcgg gcagcaagag ggcgtgttac ttgaaaagct taaagctggt gaagttagcg
     1021 ccgaaggtct gcgcattgtg ctggaagcgg gtattcgcga gccgatgatt atgcgggcca
     1081 atcaggcgct gtatgcgcaa ttacacccca taaaagaatc cattttctgg cgtcaggttg
     1141 acggcggaca tgatgcgctt tgttggcgcg gtggcttgat gcaggggcta atcgacctct
     1201 ggcaaccact tttccatgac aggagttgaa tatggcattc agtaatccct tcgatgatcc
     1261 acagggagcg ttttacattt tgcgcaatgc gcaggggcaa ttcagtctgt ggccgcaaca
     1321 atgcgcctta ccggcaggct gggacattgt gtgtcagccg cagtcacagg cgtcctgcca
     1381 gcagtggctg gaagtccact ggcgtactct gacaccggcc aatttcaccc agttgcagga
     1441 ggcacaatga gccagcattt acctttggtc gccgcacagc ccggcatctg gatggcagaa
     1501 aaactgtcgg atttaccctc cgcctggagc gtggcgcatt acgttgagtt aactggagat
     1561 gttgatgcgc cattactggc ccgcgcggtg gttgtcggac tagcgcaagc agatacgttg
     1621 cggatgcgtt ttacggaaga taacggcgaa gtctggcagt gggtcgatga tgcgctgata
     1681 ttcgaactgc cagaaattat cgacctgcga accaatattg atccgcacgg tactgcgcag
     1741 gcattaatgc tggcggattt gcaacaagat ttgcgcgtcg atagcggtaa accactggtc
     1801 tttcatcagc tgatacatgt tgcggataac cgctggtact ggtatcagcg ttatcaccat
     1861 ttgctggtcg atggcttcag tttcccggcc attactcgcc agatcgccaa tatttactgc
     1921 acatggctgc gtggcgaacc aacgcctgct tcgccgttta cgcctttcgc tgatgtagtg
     1981 gaagagtacc agcaataccg cgaaagcgaa gcctggcagc gtgatgcggc attctgggca
     2041 gaacagcgtc gtcaactgcc gccgcccgcg tcactttctc cggcaccttt agcggggcgc
     2101 agcgcttcgg cagatattct gcgcctgaaa ctggaattta ccgacgggga attccgccag
     2161 ctggctacgc aactttcagg tgtgcagcgt accgatttag cccttgcgct ggcagccttg
     2221 tggctggggc gattgtgcaa tcgcatggac tatgccgccg gatttatctt tatgcgtcga
     2281 ctgggctcgg cggcgctgac ggctagcgga cccgtgctca acgttttacc gttgggtatt
     2341 cacattgcgg cgcaagaaac gctgccggaa ctggcaaccc gactggcagc acaactgaaa
     2401 aaaatgcgtc gtcatcaacg ttacgatgcc gaacaaattg tccgtgacag cgggcgagcg
     2461 gcaggagagg aaccgctgtt cggtccggta ctcaatatca aggtatttga ttaccaactg
     2521 gatattcctg gtgttcagac gcaaacccat accctggcaa ccggtccggt taatgacctt
     2581 gaactggccc tgttcccgga tgaacacggt gatttgagta ttgagatcct cgccaataaa
     2641 cagcgttacg atgagccaac gttaatccag catgctgaac gcctgaaaat gctgattgcc
     2701 cagttcgccg cggatccggc gctgttgtgc ggcgatgtcg atattatgct gccaggtgag
     2761 tatgcgcagc tggcgcagat caacgccact caggttgaga ttccagaaac cacgctgagc
     2821 gcgctggtgg cagaacaagc ggcaaaaaca ccggatgctc cggcgctggc agatgcgcgt
     2881 tacctgttca gctatcggga aatgcgcgag caggtggtgg cgctggcgaa tctgctgcgt
     2941 gagcgcggcg ttaaaccagg ggacagcgtg gcggtggcac taccgcgctc ggtctttttg
     3001 accctggcac tccatgcaat agttgaagct ggcgcggcct ggctaccgct ggataccggc
     3061 tatccggacg atcgcctgaa aatgatgctg gaagatgcgc gtccgtcgct gttaattacc
     3121 accgacgatc aactgccgcg ctttagcgat gttcccaatt taacaagcct ttgctataac
     3181 gccccgctta ccccccaggg cagtgcgccg ctgcaacttt cacaaccgca ccacacggct
     3241 tatatcatct ttacctctgg ctccaccggc aggccgaaag gggtaatggt cgggcagacg
     3301 gctatcgtta accgcctgtt atggatgcaa aatcactacc cgcttacagg cgaagatgtc
     3361 gttgcccaaa aaacgccgtg cagttttgat gtctcggtgt gggagttttt ctggccgttt
     3421 atcgcagggg caaaactggt gatggctgaa ccggaagcgc accgtgaccc gctcgctatg
     3481 cagcaattct ttgccgaata tggcgtaacg accacgcact ttgtgccgtc gatgctggcg
     3541 gcatttgttg catcgctgac gccgcaaacc gctcgccaga attgcgcgac gttgaaacag
     3601 gttttctgta gtggtgaggc cttaccggct gatttatgcc gcgaatggca acagttaacc
     3661 ggcgcgccgt tgcataacct gtatggcccg acggaagcgg cggtagatgt cagctggtat
     3721 ccggcttttg gcgaggaact ggcacaggtg cgcggcagca gtgtgccgat tggttatccg
     3781 gtatggaata cgggtctgcg tattcttgat gcgatgatgc atccggtgcc gccgggtgtg
     3841 gcgggtgatc tctatctcac cggcatccaa ctggcgcagg ggtatcttgg acgacccgat
     3901 ctgaccgcca gccgttttat tgccgatcct tttgccccag gtgaacggat gtatcgtacc
     3961 ggagacgtcg cccgctggct ggataacggc gcggtggagt acctcgggcg cagtgacgat
     4021 cagctaaaaa ttcgcgggca gcgtatcgaa ctgggcgaaa tcgatcgcgt gatgcaggcg
     4081 ctgccggatg tcaaacaagc cgttacccac gcctgtgtga ttaacctggc ggcagccacc
     4141 ggtggtgatg cgcgtcagtt ggtgggctat ctggtgtcgc aatcaggtct gccgttggat
     4201 accagcgcat tacaggcaca gcttcgcgaa acattgccgc cgcatatggt gccggtggtt
     4261 ctgctgcaac ttccacagtt acctcttagc gccaacggca agctggatcg caaagcctta
     4321 ccgttgcctg aactgaagac acaagcgtcg ggacgtgcac cgaaagcggg cagtgaaacg
     4381 attatcgccg cggcattcgc gtcgttgctg ggttgtgacg tgcaggatgc cgatgctgat
     4441 ttctttgcgc ttggcggtca ttcgctactg gcaatgaaac tggcagcgca gttaagtcga
     4501 cagtttgctc gtcaggtgac gccggggcag gtgatggtcg cgtcaaccgt cgccaaactg
     4561 gcaacgatta ttgatggtga agaggacagc tcccggcgca tgggattcga aaccattctg
     4621 ccgttgcgtg aaggtaatgg cccgacgctg ttttgtttcc atccggcatc cggttttgcc
     4681 tggcagttta gcgtgctctc gcgttatctc gatccacaat ggtcgattat tggcattcag
     4741 tcgccgcgcc ctcatggacc catgcagaca gcgacaaacc tggatgaagt ctgcgaagcg
     4801 catctggcaa cgttacttga gcaacaaccg cacggccctt attacctgct ggggtattcc
     4861 cttggcggta cgctggtgca gggtattgcg gcgcgactgc gtgcccgtgg cgaacaggtg
     4921 gcatttcttg gcttgctgga tacctggccg ccagaaacgc aaaactggca ggaaaaagaa
     4981 gctaatggtc tggacccgga agtgctggcg gagattaacc gcgaacgcga ggccttcctg
     5041 gcggcacagc agggaggtac ttcaacggag ttgtttacca ccattgaagg caactacgct
     5101 gatgcagtgc gcctgctgac gactgctcat agcgtaccgt ttgacggtaa agcgacgctg
     5161 tttgttgctg aacgcacgct tcaggaaggt atgagtcccg aacgcgcctg gtcgccgtgg
     5221 atagccgagc tggatattta tcgtcaggat tgtgcgcatg tggatattat atcttcagag
     5281 gcgtttgaaa aaattgggcc gattattcgc gcaacgctaa acaggt
//
LOCUS       DAZGVQ010000255         5143 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000255 DAZGVQ010000000
VERSION     DAZGVQ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.258"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..327)
                     /gene="gadE"
                     /locus_tag="ACHLAG_005004"
     CDS             complement(<1..327)
                     /gene="gadE"
                     /locus_tag="ACHLAG_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFP0658081.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPH"
     gene            complement(1126..1698)
                     /gene="hdeD"
                     /locus_tag="ACHLAG_005005"
     CDS             complement(1126..1698)
                     /gene="hdeD"
                     /locus_tag="ACHLAG_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFP0658082.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            1953..2285
                     /gene="hdeA"
                     /locus_tag="ACHLAG_005006"
     CDS             1953..2285
                     /gene="hdeA"
                     /locus_tag="ACHLAG_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFP0658083.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            2401..2727
                     /gene="hdeB"
                     /locus_tag="ACHLAG_005007"
     CDS             2401..2727
                     /gene="hdeB"
                     /locus_tag="ACHLAG_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFP0658084.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            2791..3438
                     /gene="yhiD"
                     /locus_tag="ACHLAG_005008"
     CDS             2791..3438
                     /gene="yhiD"
                     /locus_tag="ACHLAG_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFP0658085.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTTAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(3480..4010)
                     /gene="dctR"
                     /locus_tag="ACHLAG_005009"
     CDS             complement(3480..4010)
                     /gene="dctR"
                     /locus_tag="ACHLAG_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP0658086.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVNFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(4166..4732)
                     /gene="slp"
                     /locus_tag="ACHLAG_005010"
     CDS             complement(4166..4732)
                     /gene="slp"
                     /locus_tag="ACHLAG_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFP0658087.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(4979..>5143)
                     /locus_tag="ACHLAG_005011"
     CDS             complement(4979..>5143)
                     /locus_tag="ACHLAG_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658088.1"
                     /translation="EVFQYETLNPKKMSHTPYLKRPLPNEKDINYGVEIEIPSGKRIR
                     LSNHYQNIIP"
BASE COUNT         1571 a         1027 c         1033 g         1512 t
ORIGIN      
        1 atggggcaag tgtttaccat aagtaatctc aagaagattt ttgatacttt ctttgcggct
       61 aacaaaataa ataggcgctt tagcttttag tttactgatg tgataaggtg ccaaaacaat
      121 aattctctcg gcatctaatt tctccagaaa tttaatcgct tcttcatcaa ggatatgatt
      181 gtgataggta tcaatgataa ccttgaaggg tttattgcct acttttttga tctctgacag
      241 ggaattgatt tttatcattt cgtgattatc tttcaactgc caaaagccct gtaaaagaaa
      301 agaatctttc gtcatgagaa aaatcataac ttgctcctta gccgttatcg tttcgaacat
      361 gtcatccttt tcattattta catccttgtc cgaatcgttg ttcaatatag taaacgccta
      421 taagaacttt gcccttcgtg aactaatacg tctattaaca ctaatcaata cttcagattt
      481 atccatcacg cactccatat attgatccta agtaagattc ctggttgtta tcaatttgta
      541 aatatcaaac aaaaagttaa cgtttgcgga ttactgccca agaataagtt atcaataacc
      601 agtttgtgat ctctgaagaa tattactaaa gttaaaatgt aatccgattt aaatatcgag
      661 tctccttgtt tcgacttaag ctggcaattg gattgccagc tttcttttaa cgcattgaaa
      721 ttacctgagt aaataacgca ttcatgtctt caggttttta tatagcgtct ggagacacgg
      781 atatttatgc aatgtaatca acgccagttg caaaaaaatg aacccggatt acttatttaa
      841 ccgcccatag catcaacgct tacggaactc tttgttcact ttcccacagc acttttaaaa
      901 cattaaacct gactacggaa aatatcagcc atgataatgt ttgaaatggc taatttgcca
      961 tggagtgaaa aaaattagat gaaattcagt aggttgaaat gatcactagc aggtaattat
     1021 ttcaatgata gtgcgcaatt gatctacaac actgcgtagc ggagagagta ttaatcggat
     1081 catagtcaca tcaagtgact atgatccggg tgacaaccgg gataattatt gctgcttaac
     1141 gaacaaactg gcgaagctga acaggctggc ggcgctaaat atcagttcaa ttcccaccag
     1201 tgtggaaacc agcgttacag acaccatcgg cgttgcacca aggaatatcc aggcaatgac
     1261 gatatccagc acaccaataa cgagctgtag ccagctgcct ttcattgaac gctgacgata
     1321 ccaactcatc aggcgaataa cccctgcaac acagaacaaa ccggcaataa atgccgcaat
     1381 ggcaaaaatg cccagctccg gtgcgcggat gaagaaatag ccgatcaata aataggcgac
     1441 tgcgacgagg aaaccggata ataccggcca gaaattatga ctgcggttgc tgaataaccc
     1501 gacaataagc gcaatacccg agcagattaa taatgcaccc actactgtgc ttaaaatatc
     1561 gccagagacg aacgggaaac tgatacacag caacccgacg ataaacagca gcacggcaat
     1621 aaactggatt gctctgcgat gctttttcag catctccaga tcaaacttca aaattgttgc
     1681 cttatctata tataacatag aaccacccta taaaattaag aagaaaatcc cctgctatca
     1741 atctatgcca aaaacgcgtc taagaatgca gtcgatttaa taaaaatttc ctaattgcag
     1801 tatctgatgc atctgtaact cattgtattg aaataaaaat atctgatttt gatattttcc
     1861 atcaacatga catatacaga aaaccaggtt ataacctcag tgtcgaaatt gattcgtgac
     1921 ggctctttca ctttatagtt gaggatatta cgatgaaaaa agtattaggc gttattcttg
     1981 gtggtctgct tcttctgcca gttgtgagca atgcagcgga tgcgcaaaaa gcagctgata
     2041 acaaaaaacc ggtcaactcc tggacctgtg aagatttcct ggctgtggac gaatccttcc
     2101 agccaactgc agttggtttt gctgaagcgc tgaacaacaa agataaacca gaagatgcgg
     2161 ttttagatgt tcagggtatt gcaaccgtaa ccccagctat cgttcaggct tgtactcagg
     2221 ataaacaagc caactttaaa gataaagtta aaggcgaatg ggacaaaatt aagaaagata
     2281 tgtaattccg ggaatgcgtt acatcgtact tccttgcata ttgaacaggc cggaatatct
     2341 tctttaaaag cagctattcc tcctgttcat atataatctc tatattgaat gggttacaaa
     2401 atgaatattt catctctccg taaagcgttt atttttatgg gcgctgtagc ggctttgtca
     2461 ctggtgaacg cacaatctgc gctggcagcc aatgaatccg ctaaagatat gacctgccag
     2521 gaatttattg atctgaatcc aaaagcaatg accccggttg catggtggat gctgcatgaa
     2581 gaaacagtat ataaaggtgg cgataccgtt actttaaatg aaaccgatct cactcaaatt
     2641 cctaaagtga tcgaatactg taagaaaaac ccgcagaaaa atttgtatac cttcaaaaat
     2701 caagcatcta atgacttgcc gaattaatga ggtacaagta aaaaggagta gcaagttgag
     2761 ccatcttgct gctccttttt gcatttttat atgacagcag aatttattat acgtcttata
     2821 ctcgcggcaa ttgcctgtgg cgctattggc atggaaaggc aaatgcgcgg caaaggagca
     2881 gggttacgca cacatgtatt aattggcatg ggaagcgccc tgtttatgat tgtttcgaaa
     2941 tatggttttg ctgacgtgct gtctttagat cacgtcggac tcgaccccag ccgtattgct
     3001 gctcaggtgg tgacgggcgt cgggtttatc ggtgcaggta acattctcgt tcgtaaccaa
     3061 aatattgtag gtctgacgac ggcagcggat atctgggtga ccaccgccat aggtatggtt
     3121 attggcagcg gtatgtacga actgggtatt tatggttcag tgatgacctt gttggtgctg
     3181 gaagtcttcc atcaattaac cttccgcctg atgaacaaaa attaccattt acagctgaca
     3241 ttagtgaacg gtaatacggt atcaatgctc gactggttta agcagcaaaa aataaaaacg
     3301 gatttagttt cgttgcagga aaatgaagat catgaagtgg tcgccattga tatccagctt
     3361 catgccacaa cgtcgataga agacctgttg cgattattaa aaggtatggc aggggtgaaa
     3421 ggcgtttcaa ttagctaatc tgtgtggcag cgtagccaga ctcaccgtaa gcctgaaatt
     3481 cacaccagat aatcaatatg ctgatggcgt accagctcat tgatccgctt aagattgagg
     3541 atcatcatga tattgtgttt atggcagtag aacgttttta gcgatatttt cagtttctcc
     3601 agaatatctt ccgttgtcat ttgattcgca atgcagctca gaacctgatg ttgcttgcgc
     3661 gaaagtgaca cagccgcttc aggcacagag cagtaactgc cattgatgag tgcgacaaaa
     3721 tcaatgatag tgatagtccg cggaacaaag ttcaccggca ctttccccag gcagcgttta
     3781 atattaaagg gggagaaaat aatcacatgg acaggtttta atcgttcaat ttgagtgaga
     3841 aaattagaat gaaatacatt attcattaat gtatcaataa tgacgaaggc attctgatgc
     3901 aacattacgt cgatctcttc ttcgttctgt atatgaacga cattaccttt actcagaatg
     3961 tttttcatcg cggtgaagaa catcgtatcc ctggtaatta taagaaacat aaccgactcc
     4021 tggtgcggac attaacttta taatcagttt gccaaaggta attaacagga cagggcttat
     4081 aataataata gttagcttac tgtatattaa ttttatgtaa tacatgatgc agatgctgag
     4141 aaagcatctg catctttcgg tggtgttatt tgaccagctc aggtgttacc tgactcaccg
     4201 cattggtgta gtaaggcgca ccccagcccg gttccggcca gaatgcacca tagccgtaat
     4261 cccacaggtt ataggtggta ttaaccactt ctctcaaatg ccacacctgg atgccctgca
     4321 tattcacttc caggaagtta tacgggactt tattgataaa gcctggttgt tcaccctgaa
     4381 tggtaccgag gatggtaaca aagtgattac gatagttcac tggatcaagg aagccgcttt
     4441 gtctggcgag cagtcggccc tgatagttgg cttcaatatc aggcttcgca tagctatcca
     4501 acggtaatac agcgatttct aacaacgtat ccgttttgcc attgataacg ttgataacct
     4561 tcccaccaaa gcgcgcttgt tgaccaacat ataaccccgg ctggttatga acagcaacaa
     4621 aacttttttg aatatcaggt tggttattgc ctttgatatt ttgcggaatt gaactacatg
     4681 cggcaagcaa aaatgaaagg ctgaggatga gtgcaccttt tgtcatgttc atgttactat
     4741 ccttatcaac aaatattaaa accataatag atatttcttt atagttttca tctgattctg
     4801 aggtgatatg cctgatagta acgtaaacct gtctgatgtt tatatccctt ctgtttagtt
     4861 caattccgtt attttggggc gtggtatgag tatccatcgc ccaataatat aaccctttgt
     4921 tttaaaatta cataaaatct atttttattc aataattata aagtgctaat aatcaatatt
     4981 atggtattat attctgataa tgatttgata agcgaattct tttgccagat ggtatttcta
     5041 tttccacccc ataatttata tctttttcat taggaagtgg cctctttaaa taaggcgtgt
     5101 gtgacatttt tttggggtta agcgtctcgt attgaaatac ttc
//
LOCUS       DAZGVQ010000256         5112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000256 DAZGVQ010000000
VERSION     DAZGVQ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.259"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..109
                     /locus_tag="ACHLAG_005012"
     CDS             <1..109
                     /locus_tag="ACHLAG_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658089.1"
                     /translation="GFEVGEYNTYEAYNSENQYFTREDYTYNYDLLNAI"
     gene            complement(504..880)
                     /gene="rnpB"
                     /locus_tag="ACHLAG_005013"
     ncRNA           complement(504..880)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHLAG_005013"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(913..2058)
                     /gene="garK"
                     /locus_tag="ACHLAG_005014"
     CDS             complement(913..2058)
                     /gene="garK"
                     /locus_tag="ACHLAG_005014"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFP0658090.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2155..3045)
                     /gene="garR"
                     /locus_tag="ACHLAG_005015"
     CDS             complement(2155..3045)
                     /gene="garR"
                     /locus_tag="ACHLAG_005015"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP0658091.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3075..3845)
                     /gene="garL"
                     /locus_tag="ACHLAG_005016"
     CDS             complement(3075..3845)
                     /gene="garL"
                     /locus_tag="ACHLAG_005016"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFP0658092.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3861..>5112)
                     /gene="garP"
                     /locus_tag="ACHLAG_005017"
     CDS             complement(3861..>5112)
                     /gene="garP"
                     /locus_tag="ACHLAG_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFP0658093.1"
                     /translation="VNYADRATLSIAGTEVAKELQLSAVSMGYIFSAFGWAYLLMQIP
                     GGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRFMLGFSEAPSF
                     PANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLTFAWGWEHVFTVMGVIG
                     FVLTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKLHYIKQL
                     LSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCGFAGGV
                     LGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNNTTLVVMLMALAFFGKG
                     FGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGYLVSELHSFNAALVFVG
                     CSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1236 a         1465 c         1222 g         1189 t
ORIGIN      
        1 tggttttgag gtcggggaat ataacacata tgaagcatat aactcagaga atcaatattt
       61 taccagagag gactatacgt ataattacga ccttttaaat gcaatataga cttaataact
      121 tcttacaaag tctattatta ataccgtaga tatttattca tcttaccctg tgcaccaaaa
      181 gcaaaaactc actgacgcgg gtttgaaact gatggcaacc gcaaaaatgc ctgatgcgct
      241 acgcttatca ggcctacgcc atctctgcaa tatattgaat ttgcgtgctt ttgtaggcag
      301 gataaggcgt tcacgccgca tccggcatga ataaagcgca ctcaacaatc tgaaacccgc
      361 cggaacggtt tattacgtac atcaggtgaa actgaccgat aagccgcttt cttttgggta
      421 tagcgtcgtg gacagtcatt catctttctg cccctccaaa agcaaaaacc cgccggttta
      481 cgctggcggg ttttcgttaa atcaggtgaa actgaccgat aagccgggtt ctgtcgtgga
      541 cagtcattca tctaggccag caatcgctca ctggctcaag cagcctaccc gggttcagta
      601 cgggccgtac cttatgaacc cctatttggc cttgctccgg gtggagttta ccgtgccacg
      661 gactgttacc agccgcgcgg tgcgctctta ccgcaccctt tcacccttac ctgatcccgc
      721 ttgcgcgggc catcggcggt ttgctctctg ttgcactggt cgtgggtttc ccccccaggc
      781 gttacctggc accctgccct atggagcccg gactttcctc ccctccgccc gtctcccccg
      841 aagaggacga cgacgaagcg gcgactgtct ggtcagcttc ggcgcgcagt atagagggtt
      901 tgcgcgccct tgtcaccccg cgttgcgcat tccaatcgcc agtgtcgcgg cgatattacg
      961 tgaagcacgg cagatattgt cataagcccc gcggaatgct tcgtccaacg tacctatgct
     1021 ggtcaatacg ctgaagaccg catcgatgcc atgctgatgt acaacgccaa catcatcggt
     1081 caggctaccc gcaatgccaa tcaccggttt atggtacttc ttcgccacgt ttgcaacgcc
     1141 aatcggtacc ttgccgtgaa tactctggct gtcaatacgc ccttcaccgg tgatcaccag
     1201 cgtacaatcg tgaatatgtt cctccagatt cagcgccgta gtgacgattt caataccact
     1261 tttcagttcc gcaccaagaa acgccatcag cgccgcaccc ataccgcccg cagctcctgc
     1321 gccggggaca tctttcacat caacatgcag cgctttttta atgacctcgg catagtgaga
     1381 gaggttattg tccagctcaa caatcatcgc ttcactggct cccttttgtg ggccaaagat
     1441 gcgcgatgcg ccgttatcgc ccaccagcgg attggtgaca tcacaagcga cgcgaatgac
     1501 gcaatctttt aagcgcggat cgaggccgga aatatcaata tcattcagag tattaagact
     1561 accgccgcca aaaccaattt cattgccgtt ggcgtcgcat aatttcgccc ccagcgcctg
     1621 taccatgcct gcgccgccat catttgtagc gctaccgcca atgccgataa taatgtttgt
     1681 cgcgccgctc tccagcgcct gcaggattaa ctcgcctgtg ccgcgtgaag tggtcacgag
     1741 tggatcgcgt ttttccgcag gtaccagctc cagcccactg gccgccgcca tttcaataaa
     1801 cgcggttttg ccatcgccgg agatccccca actggcattc actttctcgc ccagcggccc
     1861 tgtaacccag gcgtgacgtt cagaaccctg ggtggctgca atcatcgctt ccaccgttcc
     1921 ttcgccaccg tcggcaaccg gaatagaaac gtactgtgca tcaggaaaaa tttcccgaaa
     1981 tcctttttct atcgcctgcg caacctcgct ggcagataaa ctttctttat aagagtctgg
     2041 ggcgattacg attttcatac ctatgcctgt taccacatga cgccggaggg cgtttctctt
     2101 attcggcctg gattccaggc ccggattgca atacgccatc cgggcacgac gtcattaacg
     2161 agtaacttcg actttcgcca gtttttcgta gtagcacgcc agggcgctat gatccgccgt
     2221 tcctaaacca tctgctcgca gtgcctgcat catctccata accgcagctg tgagcggcag
     2281 ttgcgcgccg acgccgtggg aagtatccag cgcatttgcc agatccttaa tatgcagatc
     2341 aatacggaag cccggcttga agttgcggtc catcaccatc ggcgctttgg catccagcac
     2401 ggtactgccc gccagtccac cgcgaattgc ctgatacacc aggtccgggt taacgcccgc
     2461 tttggttgcc agcgttagcg cttctgacat cgcggcaata ttcagcgcca caatgacctg
     2521 atttgccagt ttggtgacgt tacctgcacc gatttccccg gtatgcacca cggaacccgc
     2581 catcgctttc atcaaatcat agtatttgtc gaaaatagcc ttgtcgccgc ccaccatcac
     2641 tgacagcgta ccgtcgatgg ctttcggttc accgccgctc accggagcat ccagcatatc
     2701 aatgcctttc gctttcagcg cttcgctgat ttcacggctt gccagcggtg cgatagaact
     2761 catatcgatc aataccgtac ctggcttcgc gccttcaatg atgccattct cacccagcgc
     2821 cacctctttc acatgagggg agtttggcag catggttatg atgacgtcgc actgttcagc
     2881 gatcgcttta gccgtagacg ctgtttctgc acctgcagca atcacgtcag caatagcttc
     2941 tgggttacgg tcagcaacca ccagcgagta acctgctttc agaaggtttt tactcattgg
     3001 tttacccata atccccaggc caataaaacc aactttcata gtcatatcaa tcatctctct
     3061 tgttgcggtg gtggttattt tttaaaggta tcagccagtt tctgagtggc agagcggaag
     3121 acgccaagat cgctgccgac agccacaaac gtcgcgcccc attccagata acgacgcgca
     3181 tcggcttcga ccggcgcgag aataccgctg ggtttgccgt gcgcactggc acggttaaaa
     3241 atgtgctgaa ttgctttttg tacatccggg tgcgaggcat tgccgagatg gcctaatgcc
     3301 gcagccagat cgctggggcc gacgaagata ccgtctacac cttcggtagc ggcaatggca
     3361 tcgacgttat ctacgccctg ctgactttct atctggacca gaatggtgat gttcttgttc
     3421 gactgagcga aataatccgc cacggtgcca aacatattgg cgcggtgaga aacggagacg
     3481 ccgcgaatgc cttccggtgg gtaacgggtt gatgccaccg cctgctctgc ttcctctttt
     3541 gtttctacaa aaggaatcag gaagttatag aaaccgatat ccagaagacg cttaataatt
     3601 accggctcgt tggtcggcac tcgcactact ggcgcgctgg cgctgccttt cagggccatt
     3661 aactgcggaa taaacgtgga gatatcgttt ggcgcatgtt cgccatccag caccagccag
     3721 tcaaacccag ccaaaccaag aacttcagtg ctaatcgggt tagagagtgc tgaccagcaa
     3781 ccaatttgta cctgtttcgc agccagtgcg gctttgaatt tattcgggaa aacatcgtta
     3841 ttcatcgctt atacctttgc ttatttctgc aattccatac gtttaatgtc gccaactacg
     3901 aagaggtagc agaccatcgc catcagcgct gaacatccca cgaaaaccag tgctgcattg
     3961 aaggagtgca gttcacttac caggtagcca atcaccagtg gagtgacaat ggaggcaaca
     4021 ttgccaaaga cgttaaagac gccgccacag aggccaacaa tctctttcgg cgcggtgtca
     4081 gaaatcaccg gccagcccag agcaccaaat cctttgccaa aaaaagccag cgccattagc
     4141 atgaccacca gcgtggtgtt gttggtgtag ttacataaga tgatggtgga agccagcaac
     4201 attcccagca caatcggtag cttacgtgcc agggtcaggg ataaaccgcg tttgatcaga
     4261 taatccgaga agacacctcc cagcacgccg cccgcaaaac cacacagtgc cggaatcgag
     4321 gcgaccagac ccactttcag aatcgacatg cctttttcct gcaccagata aatcgggaac
     4381 caggtgagga agaaccaggt gatggtgttg ataaaatatt gtccgaaaaa tacgcccaac
     4441 atcatgcggt tagagagcaa ttgcttgatg taatgcagtt tgggaccgct tgctgccgca
     4501 ctgcccggct ttttgtggtc catatcgacc accgcgccat tttcagagat aaacttcagc
     4561 tcttccgcag acatacgtgg gtgatctgtc gggttatgaa tcaatttgat ccacagcgcc
     4621 gtcagcacaa aaccaatcac ccccataacg gtaaagacgt gctcccagcc ccaggcgaaa
     4681 gtcagccagc caagcagagg cgaaaagagc gccagcgaga aatattgcgc cgagttaaag
     4741 atggcggagg cagtaccacg ttctttcgtc gggaaccagg cggcgacaat acgggcgttc
     4801 gccgggaatg atggcgcttc cgagaagccg agcataaagc gcataaagaa catggagatc
     4861 cctgcccagg ccagcgggaa catatcaaca aagccttgca ggaaagtgaa tagcgaccag
     4921 aaaaagaggc tataggtata aacttttttc gagccaaact tatcaagcag ccagccgccg
     4981 gggatttgca tcagcaagta ggcccagcca aaagcagaaa agatgtaacc catcgaaacc
     5041 gcacttaact gcaactcttt tgccacttcg gtaccagcaa tagacagcgt tgcacgatcg
     5101 gcgtagttaa cg
//
LOCUS       DAZGVQ010000257         5069 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000257 DAZGVQ010000000
VERSION     DAZGVQ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.260"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            74..1990
                     /gene="mngA"
                     /locus_tag="ACHLAG_005018"
     CDS             74..1990
                     /gene="mngA"
                     /locus_tag="ACHLAG_005018"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFP0658094.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVMG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            2008..4641
                     /gene="mngB"
                     /locus_tag="ACHLAG_005019"
     CDS             2008..4641
                     /gene="mngB"
                     /locus_tag="ACHLAG_005019"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFP0658095.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLLERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNSMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLFLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         1256 a         1177 c         1376 g         1260 t
ORIGIN      
        1 aattgatcac ataatggtat tgttttatcg ggcacactgg cgcgactata aaaacgatca
       61 agtgaggatc atgatgaatc tgacgactct gacccaccgc gatgcgttgt gtctgaatgc
      121 gcgctttacc agccgtgaag aggccatcca cgcgttgact caacgtcttg ctgctctggg
      181 gaaaatttcc agtactgagc aatttctgga agaagtgtat cgccgtgaaa gccttggccc
      241 gaccgcctta ggtgaagggt tggctgtgcc gcatggcaaa actgctgcgg taaaagaagc
      301 ggcgtttgcg gtcgccacac tcagcgagcc gcttcagtgg gaaggcgttg atggcccgga
      361 agcggttgat ttagtggtgc tgctggcgat tccccccaat gaagcgggta ctacgcatat
      421 gcaactgctg acagcgctga ccacgcgcct ggcggatgat gagattcggg cgcgtataca
      481 gtcggcgacg acgcctgatg agttgctctc ggcgctggat gacaagggag gcacgcaacc
      541 ttctgcctct ttttccaacg cgccaactat cgtctgcgta acggcctgtc cggcgggtat
      601 tgctcacacc tatatggctg cggaatatct ggaaaaagcc ggacgcaaac tcggcgtaaa
      661 tgtttacgtt gaaaaacaag gcgctaacgg cattgaaggg cgtttaacgg cggatcaact
      721 caatagtgca accgcctgta tttttgcggc tgaagtcgcc atcaaggaga gtgagcgttt
      781 taatggcatt cccgcgcttt cagtgcctgt tgccgagccg attcgccatg cagaagcgtt
      841 gatccaacaa gcgcttaccc tcaagcgtag cgatgagacg cgtaccgtac agcaagatac
      901 gcaaccggtg aaaagtgtca aaacggagct gaaacaggca ctgttgagcg gaatctcttt
      961 tgccgtaccg ttgattgtcg cggggggcac ggtgctggcg gtcgcggtat tactgtcgca
     1021 aatcttcggg ctacaagatc tgtttaatga agaaaactcc tggctgtgga tgtaccgcaa
     1081 gctgggcggc gggctgctcg gaattttgat ggtaccggtg ctcgcggcct ataccgccta
     1141 ttctctggca gataaaccgg cgttagcgcc aggctttgcg gctggacttg ccgccaacat
     1201 gatcggctcc gggtttctcg gcgcggtcgt tggcggattg atagccggtt acttgatgcg
     1261 ctgggtgaaa aatcacttgc gtcttagcag taaattcaat ggattcctga ctttttatct
     1321 ctacccggtg ctcggtacgt tgggagcggg cagtctgatg ctgtttgtgg tgggagaacc
     1381 tgtcgcctgg atcaataact cgcttaccgc ctggctgaac ggtctgtcag gaagtaacgc
     1441 gctgttgctg ggtgccattc tcggttttat gtgttccttt gaccttggag ggccagtgaa
     1501 caaagccgct tatgcattct gcctgggcgc aatggcgaac ggcgtttacg gcccgtatgc
     1561 cattttcgcc tccgtcaaaa tggtttcggc atttaccgta accgcttcca cgatgctcgc
     1621 accgcgcctg tttaaagagt ttgaaattga gaccgggaaa tccacctggc tgttagggct
     1681 ggcaggtatt accgaagggg cgatcccgat ggcgattgaa gatccgctgc gggttattgg
     1741 ttcgtttgtg ctgggctcta tggtaatggg cgctattgtc ggtgcgatga atatcggcct
     1801 ttcgacaccc ggtgccggca ttttctcgct ctttttactt catgataatg gcgcgggcgg
     1861 tgttatggcg gcaattggct ggtttggtgc ggcattggtg ggggctgcaa tctcgactgc
     1921 aattctcctg atctggcggc gtcacgcggt taagcatggc aactatctga ctgatggcgt
     1981 aatgccataa acaaaaagga aacgacgatg aaagcggtat ctcgcgttca catcaccccg
     2041 catatgcatt gggatcggga gtggtatttc accaccgaag agtcacgcat tctgctggtc
     2101 aataatatgg aagagatcct gtgccgactg gaacaggaca acgaatacaa atattacgta
     2161 ctcgacgggc aaacggcgat cctcgaagat tatttcgcgg tgaaaccgga aaacaaagac
     2221 cgcgtgaaga aacaggtaga agccggcaag ttgattatcg gcccctggta tacccagacc
     2281 gataccacga ttgtttctgc ggaatccatc gtccgtaatc tgatgtacgg aatgcgtgac
     2341 tgcctcgcgt ttggcgagcc gatgaaaata ggttatttac cagattcctt tggcatgtcc
     2401 gggcaactgc cgcatatcta caatggattt ggcattaccc gcaccatgtt ctggcgcgga
     2461 tgttcggagc gccacggtac tgataaaacc gagtttttgt ggcaaagcag tgacggtagc
     2521 gaagtgacgg cgcaggtgct gccgctgggc tacgccatcg gtaagtactt acctgccgac
     2581 gaaaacggat tacgtaaacg cctcgacagt tattttgacg tgctggaaaa agcgtctgta
     2641 accaaagaga ttttgctgcc gaatgggcat gaccagatgc cattgcagca aaatatcttc
     2701 gaagtgatgg ataagctacg tgagatctac cctcaacgta agtttgtgat gagccgcttt
     2761 gaagaggtat ttgagaagat cgaagcgcag cgtgaaagtc tggccaccct gaaaggtgaa
     2821 tttatcgatg gcaaatatat gcgcgtacat cgcaccatcg gttccacgcg catggatatc
     2881 aaaattgccc acgcacgtat tgaaaataag attgttaatc tgctggaacc gctggcaacg
     2941 ctggcctgga cgttgggttt tgaataccac cacggcttgc tggagaaaat gtggaaagag
     3001 atcttaaaaa atcatgccca cgacagtatc ggttgttgtt gcagtgacaa agttcatcgc
     3061 gaaattgtcg cccgcttcga actggctgaa gacatggcgg ataatctgct gcgtttctac
     3121 atgcgcaaaa ttgccgataa catgccacag agtgatgccg acaaactcgt gctgtttaac
     3181 ctgatgccct ggccgcggga agaagttatt aacaccactg tgcggctgcg cgccagccag
     3241 tttaatctac gagacgatcg cggtcagcct gtaccgtatt ttattcgcca tgcccgtgag
     3301 atcgatccag gcctaatcga tcggcaaata gttcattacg gtaattacga tccctttatg
     3361 gagtttgata tacagatcaa ccagattgtc ccttctatgg gctatcgcac gctttatatc
     3421 gaagcgaatc agcctggcaa cgtgattgcg gcaaaaagtg acgctgaagg gatactggaa
     3481 aatgctttct ggcaaattgc gctcaatgag gatggaacgc tacaactggt agataaagat
     3541 agcggtgtgc gctatgaccg ggtgttgcaa attgaagaaa gctctgatga tggtgatgaa
     3601 tatgactatt cacccgcaaa agaagagtgg gtaatgactt ctgcaacagc gaaaccgcaa
     3661 tgcgagatta ctcatgaggc ctggcagagc agggctgtta tccgctatga catggcggtg
     3721 ccgctcaatt tgttggaacg aagcgtccgg caatccacgg gcagagtagg ggtggagatg
     3781 gttgtcactc tgagtcataa cagcagacgt attgatgtgg atatcaacct tgataaccag
     3841 gctgacgatc atcgccttcg cgtcctgatc cctacatctt ttaacaccga tagtgttctg
     3901 gcagataccc agtttggttc gctaacgcgc cccgtgaacg acagtgcaat gaacaactgg
     3961 cagcaagaag gctggaaaga agcgccggtt ccggtatgga atatgctcaa ctatgcagcc
     4021 ttacaggaag ggcgtaacag catggctgtc tttagcgaag ggctacgtga atttgaagtc
     4081 atcggtgaag agaagaaaac ctttgccatt acgttgctgc gtggcgtggg cttactgggc
     4141 aaagaagatc tgtttttaag gcctgggcgg ccttcgggaa ttaaaatgcc agtcccggac
     4201 tcacaactac gtggtctgct ttcttgtcgc ctaagtttat tgagttatac cggtacgcca
     4261 accgccgctg gtgtagctca gcaggcgcga gcatggctga ctccagtaca gtgttacaac
     4321 aaaatcccat gggatgcgat gaagctcaac aaagccggat tcaacgtgcc ggaaagttat
     4381 agtttgttga aaatgccccc agtgggatgc ctgataagcg cacttaagaa agctgaagac
     4441 cgacaagaag tgattttacg gctgtttaat ccggctgaat cagcaacctg tgatgcgact
     4501 gttgctttca gtcgcgaggt gatttcttgc tcagaaacga tgatggatga acacattacc
     4561 accgaggaaa atcaaggttc aaatctatcg gggccttttt tacccggcca gtcacggacg
     4621 ttcagttacc ggcttgcctg aatagcaatc aaaccgaagc cacatatgcg cggccagatt
     4681 gttgacaaag ggcgctttgt tcatgccgga tacggcatga acgctttatt cggtctacaa
     4741 aagcaggcaa attcaatata ttgcagagat gatgtagaca ctggcaagcg tagcgcatca
     4801 ggtaattttg cgtttatctt cactctcaag ccacgtatat gtggctttat ttttaacaaa
     4861 ataataaccc tgggtgagtt aattataata taattataag ttaattaaat gttaatatca
     4921 gcggggtgga tttatgcctt tattagtaat cctgaaactc tgcgtcgtat tagccagtga
     4981 ccaaaaaaag aattaaggtc aaccgtgctg tttttgcttc gtctcttttt atctttaatt
     5041 gccaaccgaa actaatttca gccttataa
//
LOCUS       DAZGVQ010000258         4940 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000258 DAZGVQ010000000
VERSION     DAZGVQ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.261"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(67..828)
                     /gene="kduD"
                     /locus_tag="ACHLAG_005020"
     CDS             complement(67..828)
                     /gene="kduD"
                     /locus_tag="ACHLAG_005020"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFP0658096.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(858..1693)
                     /gene="kduI"
                     /locus_tag="ACHLAG_005021"
                     /pseudo
     CDS             complement(858..1693)
                     /gene="kduI"
                     /locus_tag="ACHLAG_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(1980..3161)
                     /gene="yqeF"
                     /locus_tag="ACHLAG_005022"
     CDS             complement(1980..3161)
                     /gene="yqeF"
                     /locus_tag="ACHLAG_005022"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFP0658097.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3416..4645
                     /gene="yqeG"
                     /locus_tag="ACHLAG_005023"
     CDS             3416..4645
                     /gene="yqeG"
                     /locus_tag="ACHLAG_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP0658098.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1306 a         1256 c         1069 g         1309 t
ORIGIN      
        1 ttatgatgat ctacaggtat aaaaaaccct gccatgcggc agggtcataa aagtaagaag
       61 aatgaattaa cgcgccagcc aaccgccatc cacggcaatg gtataaccat tcacataatc
      121 tgaagcgctg gaggcaagga acactatcgg ccccatcagg tcactcggca gtccccaacg
      181 accagctgga atgcggtcga gaatttccgc gctacgttgt tcatctgccc gtagttgttg
      241 agtattgttg gtcgccatgt aacccggagc tatcgcatta acattaatgt tgtgtttagc
      301 ccattcgttc gccatcaatc gcgtcacacc catcacgccg ctttttgatg cggtataaga
      361 aggcacacgg atcccgccct ggaaggagag cattgacgcg atattgataa tcttgccgcc
      421 attgccttgc gcgataaagt gtttcgccgc tgcctgagac atgaagaata cgctcttgat
      481 attcaggttc atgacatcgt cccagtcctt ttcgctgaac tcgagagcat cttcgcggcg
      541 aatcaatccg gcgttattca ccaagatatc aatatgacca aactccgcta ccgcgcgatc
      601 cagcagtgct ggaataccat caatctttcg cagatcggcg gtcaggctta aaaaacgacg
      661 ccccagcgcc gtgacctgct tgatggtttc agtaggttca acgatgttaa tgccaacaat
      721 gtcacagccc gcttgcgcca gccccaacgc catcccctgg cccagcccag tatcacagcc
      781 agtgacgacc gcaactttac cttcgagaga aaatgcactt aaaatcataa caatacctta
      841 ttcgtttatg cccacaacta gcgcaaatct ttaacggcca catggtccat atcatcaaag
      901 acctggtttt caccgaccat gccccagata aaggtataag ctttggttcc gacgccggaa
      961 tgaatcgacc agctcgggga gatcaccgcc tgctcgttat gcatcacaat atgacgcgtt
     1021 tcttgcggct gccccatcat gtggaaaacg caggcgtcat catccatatt gaaatagaaa
     1081 taaacttcca tccggcgctc gtgagtgtga cacggcatgg tgttccacaa gttacctgga
     1141 gccagctccg tcagccccat actcaattgg caggtttcca gcacatccgg gacaaaatat
     1201 ttgttaattg tgcgacggtt actggtgagg ttatcgccta acgtgactgg agatacttcg
     1261 tccggtgtga ctttttggtg ggatacgtcg tatgcgcggg tgcgcaattg taataaaact
     1321 ttgccggagt ggcggtatca atactggcaa agacaacttc ttttgcacct ttacccacat
     1381 acagggcgtc gcggtgaccg atttcatagc attggccatc gacagtaatc gtaccggcac
     1441 cgccaatatt gataacacct aactcgcgac gttcaaggaa atagcttacg ccgagttgtt
     1501 taccaacttc cccgccaacg gaaaccgttt tagttatcgg cataatgccg ccaacaataa
     1561 ttcggtcaat gtggctgtaa accatggtgt actcatcggc gacaaatacc tcttcaacca
     1621 aaaattcatt gcgcagcccc tgggtatcca gcgtttttgc gtgcgcactg tggatgctct
     1681 gtcttacgtc cacatcaacc tccgataatc agtgaagcgt gaaaatagaa aacgatagtg
     1741 aaacactgtt tcgttttctc tataaaggga tgatatgccc atgggctagg gttttcaatt
     1801 aaaaataaaa caatgtttca tttttaagtt aaggattaaa aaaagtgcag tgtcgatcac
     1861 gaatatgtca tgagaatgga aaaatgaggg agcccagaaa atgtatgtcg acttgccagc
     1921 catcattatt caacagatta atgacggcag atatttatcg gtaaggagga aatgcagagc
     1981 tactcgtcac gttcaatggt caatgccacg ccctggcccc cgccgataca aagcgttgcc
     2041 agccctttgc gggcattacg tttcaccatt tcatgaacca gagaaaccag gattcggcaa
     2101 ccggaagcgc ctatcgggtg accgagtgcg atcgcgccac cattgacatt gacccgacgc
     2161 tcatcccact caagcatctt gccaaccgaa agcgcctgtg cagcaaacgc ttcattagcc
     2221 tcgataagat cgacatcagc caactgccag cctacacgct ccaggcaacg gcgggtcgca
     2281 tacaccggcg caattcccat caatgccgga tctacaccaa cgctggcaaa tgcgcggatg
     2341 cgggccagca cgggtaaatt caacgctcgt gctttggctt cgctcatcat cattaccgca
     2401 gctgcgccat cgtttatgga tgatgcattc cccgcggtca cagaaccgag actatcaaat
     2461 gaaggattta aacgggctaa accttctgca ctggcgtcag tgcgtggctg ttcatcggta
     2521 tcaacaacca acgtctgacc gttactttgg atcattaccg ggacgatctc atctttaaat
     2581 cgtccggcgt caatcgccgc tcgcgctttt tgttgcgagc taagtgcgta agcatcctgc
     2641 aactgacggc tgatgccata ttcgcgagcc agattttcgg cggtgacacc aatatgataa
     2701 tcattgaagg catcccacaa cccatcatgc accagactgt caaccaactg gctattgcca
     2761 agctgtgcac cggtgcggct atcagtcaga acatgtggtg cgcggctcat gttttcctgg
     2821 ccaccggcga tgacaatatc agcctcgcca cactgtatcg cctgagtagc cagatgcagt
     2881 gctttaagcc cagaaccgca aacgtcatta atagtgattg cagaaacgct attaggaaga
     2941 ccacctttaa tagccgattg ccttgccgga ttctgccctg cccctgcagt caacacctga
     3001 ccaagaatta cttcatccac cgcatatgca ggaacgccgg tacgttctat taacgctttc
     3061 acgaccagac taccaagttc cacggcggaa tgacccgcta acgcaccgcg aaagcagccg
     3121 ataggtgtcc gtaacgcccc gacaatcaca acgtctttca tcacaacctc gcaccaatta
     3181 acagtgcaat agtaaatgat tgttaattac agttatctta attgtttaaa aaaagtgatt
     3241 tttatcacaa aggaaatatg cctgagcagt agtcagagtc ataactggca cgtaaggttt
     3301 gcaaccacta acccaccaat agaggggtag atagggcgtt aatctcccat acttaacctg
     3361 gtttatggta aattgccctc cattttgttt aatttgtaga tgatacgttc agataatgtc
     3421 taatatttgg tctaaagaag aaactctgtg gagtttcgcg ctctacggca cagccgttgg
     3481 tgcaggcacg ctcttccttc ctattcagtt aggttcggca ggggctgtgg tcctgtttat
     3541 tactgctctg gtcgcctggc ctttaacata ttggccacat aaagccttat gccagttcat
     3601 cctctcatcg aaaacatcag caggtgaagg gataacgggc gcagtaacac actactatgg
     3661 caagaagatt ggtaatctga ttaccacgct gtacttcatc gccttttttg tcgtcgtgtt
     3721 gatatatgca gtggcaatta ccaactcact tacggaacag ctggcaaagc atatggttat
     3781 tgatcttcgc atccgtatgt tggtgagtct gggtgttgta ttaatcctga atctcatttt
     3841 tctgatggga cgccatgcaa ccattcgggt aatgggtttt ttggtattcc cattgattgc
     3901 ctatttctta tttctttcca tttaccttgt cggtagttgg caacctgatc tattaacgac
     3961 ccaggtagag ttcaatcaga atacccttca ccagatatgg atatcgattc ccgtgatggt
     4021 tttcgccttt agccatacgc ccattatttc tacgtttgcc atagacagac gtgaaaaata
     4081 tggcgaacac gctatggata aatgcaaaaa aattatgaaa gtcgcttatc tcatcatctg
     4141 cataagtgta ctgttctttg tctttagctg cctgctttct attccacctt cgtatattga
     4201 agcggctaaa gaagaagggg tcaccatttt atcggcgctt tctatgctgc cgaacgcccc
     4261 agcatggctg tcaatttccg ggattattgt cgcagtagtt gcgatgtcga aatcattcct
     4321 gggtacgtac tttggcgtta ttgaaggtgc cacggaggtc gtcaaaacaa cactacagca
     4381 ggttggtgta aagaaaagtc gtgcatttaa ccgcgcacta tcaattatgt tggtatcgct
     4441 gatcaccttc attgtttgtt gcattaaccc gaacgcgatt tcgatgattt acgcgatcag
     4501 cggcccgctc attgccatga tacttttcat catgcctacg ttgtcaacgt atctgatccc
     4561 ggcgcttaaa ccctggcgtt ccatcggaaa tctgattacg ctgatcgtgg gtatcctgtg
     4621 cgtatcggta atgttcttta gctgatatcc attgtgaatg gttcaaaatt gctacaattt
     4681 tgaaccattc cagttttcat aatgttcatt cgttattatt ccgcactatc acttccctca
     4741 tcatatccta gtattatcag ttagttaaac acacaaagag atctgaacat cttaaataag
     4801 actatttaag atgcataact tagattcgca agatatcttc cggtcattca aaaacaattt
     4861 gcgatataga tattctcata ccatgagata gttatctgaa ccaccctcaa ccaggtaaga
     4921 ttaaatcagt gaaacataat
//
LOCUS       DAZGVQ010000259         4929 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000259 DAZGVQ010000000
VERSION     DAZGVQ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.262"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            306..1280
                     /gene="hemB"
                     /locus_tag="ACHLAG_005024"
     CDS             306..1280
                     /gene="hemB"
                     /locus_tag="ACHLAG_005024"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFP0658099.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1386..2237)
                     /gene="tauD"
                     /locus_tag="ACHLAG_005025"
     CDS             complement(1386..2237)
                     /gene="tauD"
                     /locus_tag="ACHLAG_005025"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFP0658100.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2234..3061)
                     /gene="tauC"
                     /locus_tag="ACHLAG_005026"
     CDS             complement(2234..3061)
                     /gene="tauC"
                     /locus_tag="ACHLAG_005026"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFP0658101.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPLQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(3058..3824)
                     /gene="tauB"
                     /locus_tag="ACHLAG_005027"
                     /pseudo
     CDS             complement(3058..3824)
                     /gene="tauB"
                     /locus_tag="ACHLAG_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
     gene            complement(3837..4799)
                     /gene="tauA"
                     /locus_tag="ACHLAG_005028"
     CDS             complement(3837..4799)
                     /gene="tauA"
                     /locus_tag="ACHLAG_005028"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0658102.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1174 a         1406 c         1239 g         1110 t
ORIGIN      
        1 tgatacatac gcgttataaa tatcaaaaac acaaaccaga taaatatctt atcaatatac
       61 aacaaagttt gaattattca atttatagca gcacaaccaa aggccaaaaa aaataataac
      121 caagagctat aaataaagat taaaaatatt ttcagtgaat atgaaattat tgctatttct
      181 attacaaaga tgtcctagtc tctctttcat tctcttcttg tcaacatcac gacaactttc
      241 gtaaaacact cctgccccgc ttcaggtata ctgtgcacct taattccaca aacatcaggc
      301 agaccatgac agacttaatc caacgccctc gtcgcctgcg caaatctcct gcgctgcgcg
      361 ctatgtttga agagacaaca cttagcctta acgacctagt gttgccgatc tttgttgaag
      421 aagaaattga cgactacaaa gccgttgaag ccatgccagg cgtgatgcgc attccagaga
      481 aacatctggc acgcgaaatt gaacgcatcg ccaacgccgg tatccgttcc gtaatgactt
      541 ttggcatctc tcaccatacc gatgaaaccg gcagcgacgc ctggcgggaa gatggactgg
      601 tggcgcgtat gtcgcgcatc tgcaagcaga ctgtgccaga aatgatcgtc atgtcagaca
      661 cttgtttctg tgaatacact tctcacggtc actgcggtgt gctgtgcgag catggcgtcg
      721 acaacgacgc gactctggaa aatttaggca agcaagccgt ggttgcagct gctgcaggcg
      781 cagacttcat cgccccttcc gccgcgatgg acggccaggt acaggcgatt cgtcaggcgc
      841 tggacgctgc aggctttaaa gatacggcga ttatgtcgta ttcgaccaag ttcgcctctt
      901 ccttttatgg tccgttccgt gaagctgccg gaagcgcatt aaaaggcgac cgcaaaagct
      961 atcagatgaa cccaatgaac cgtcgcgagg cgattcgtga atcactgctg gatgaagccc
     1021 agggcgcaga ctgcctgatg gttaaacctg ctggagcata cctcgacatc gtgcgtgagc
     1081 tgcgtgaacg tactgaattg ccgattggcg cgtatcaggt gagcggtgag tacgcgatga
     1141 ttaagttcgc cgcgctggcg ggtgctatag atgaagagaa agtcgtgctc gaaagcttag
     1201 gttcaattaa gcgtgcgggt gcggatctga ttttcagcta ctttgcgctg gatttggctg
     1261 agaagaagat tctgcgttaa tttttttcct ctcagaaagg gggagttaac ctgttgaaag
     1321 tacggtgaac gccccctcac cctaaccctc tccccaaggg ggcgagggaa ccgtccgcac
     1381 tcgtatcacc ccgcccgata aaacggttta tccccaagaa tcgtcgcccg atgcataatc
     1441 cgccgctgtg gcaggtaatc ggcattggca tagtgctgag tcacgcggtt atcccaaatc
     1501 gcaatatcat tcggttgcca gcgccaacgc acctgaaact ccggtttagt gatatgggcg
     1561 aataaaaagc ctaacaaggc ttcgctctct ttctcgctga catcgacaat tcgcgtagta
     1621 aagccttcat tcacaaacag cgcctgttta ccgctcaccg gatgcgttcg caccaccgga
     1681 tgtagcaacg gaggattttt cgcgaccgcc tcgcgccagc gttgatgttc ctcctcggtt
     1741 ttgcggtatt tgtattccgg gaacgattta cggaaatcat gctccgcacg cagcccgctc
     1801 agcagctggc ggaagggaac agagagcgcc tcataggccg caataccgct ggtccagagc
     1861 gtatcaccgc cggtcgaagg taattcttta gctgccagaa tcgcccctgc gggtggcgtt
     1921 tcaataaatg tcacatcggt atgccagttg tcgttatccg gcggattatc gttatgggta
     1981 tccagcacga tgatctcgtc aaccccttcg gcatgagggt aaacagggtg aatatgcagt
     2041 tcgccaaaac gctgggccag cgcgcgttgc tgctgcggcg taatagtttg atcgcgcaga
     2101 aacaccacct gatggcgcag caccgcatgg taaagctgtt caaactgatt atcgcttaac
     2161 gggcgcgtca ggtcggcacc cgaaatttgt gcgccgatat acggccccag cggggtaatg
     2221 ctcagacgtt cactcattgt acttctccat gccagggcgt cagacggcgc tgtaacgcgc
     2281 gcaaacccag ttctaaaaga aaggcgataa tcgcaatcac cgcgatcccc gccagcacca
     2341 cgtcagtcgc gagaaattca cccgctgact gaaccataaa tcctaaaccg cgcgtcgcgg
     2401 caatcagctc cgccgctacc agcgtagacc agcccacacc cagaccaatg cgtaatccgg
     2461 tgaggatttc cggcagcgcg ccgggcaaaa tgacaaacca cagcacctgc gcacggctgg
     2521 cacccagcga ctgggcggca cgaatgcgaa cctgctgcgc gcttttcacc cccgccagcg
     2581 ccgacatcgc caccggtgca aaaatcgcta aatagatcag taagatcttc gaggtttcac
     2641 caataccaaa ccagatcacc atcagcggca aataagccag cggcggcacc ggacgataaa
     2701 gttcgattac cggatccaga atgccgcgca ccgtcgggct tagccccatg gcgatcccga
     2761 ccggaatacc gatcaccacc gccgcgagta gcgccagcac aatgcgcgtc aggctggctg
     2821 ccagatgctg ccacagcgtg gcgtccataa atccctgcgg tccggcaatg gtgagcagtt
     2881 ttgccagtac ttgttgcagc ggcggcaaaa acagcgggct aatcagttgc agcgccgcca
     2941 ccgtccacca tacagtgagt aaaaccgcta acgtgccaat gcttaaggtc acctgacgcg
     3001 agagcggcca gcgccatttc agccgccgcg aatgcagttt ttcattaatg agcacactca
     3061 tgagaacgcc tcccgttgct caaatacgcg gcttaaaaca tattcgcgca tggcgatgaa
     3121 ttgtggatcg gacttgatgc tgcggctcga ctctcccgca acaaagcggc gagcaaagtt
     3181 gagcggcagc cgctccagca cacggccagg gccggatgaa agcagaacca gttcagtcgc
     3241 cataaacacc gcttcttcta tatcgtgggt aatcaacagc actgcttgcc cgtctcctgc
     3301 cagagtttca gcagcagagt ttgcatctgg tcgcgggtga aggcgtccag cgcaccaaac
     3361 ggttcgtcga gtaataacag ctggggattc gccgccagcg cgcgggcaat ccccacccgc
     3421 tgacgttgac caccggaaag ctgccagata tagcgttttt ctgcgccttc cagccccact
     3481 tttttcagca tctggtgcgc gatttccagt cgctgcattt tctctatacc tgccaattgc
     3541 aggccgaacg ccacgttgtc ctgtacattg cgccacggta gtagcccttc attctgaaaa
     3601 accaccccgc gctctgctcc cggtccctca atacgcttac ccgccagttg aatgctgcca
     3661 tgctgataag gcacaaaacc ggcaatcaga ttcagcaacg tggttttacc gcagccggac
     3721 ggccccagca ccaccagtag ctcgccgctt tccagcgtca ggttgatatc ttccagtgcc
     3781 ggtttgccgc cataatcggc atagagatga gagatttgca gcatctgcgc ctccttttat
     3841 tgcacgaagc gcgaggtaac gtactggctg taatcattcg ctacagccgg gaccttgccc
     3901 tgctctttca aaaactgcgc ggtgtcgatg attgctttgt tcaccggtcc ggtcagttct
     3961 gccgtttgtt gctgcggcgt cagataggta tttcccttca ccagccccgg aacgtcacct
     4021 tcaggcacgc cgcttaaacg cgccagtttg ctgatgtttt ccggctgttt cagccacgcg
     4081 tctgggttag caatgtacgg ttgctgagca tcgatggcgc ttttagcgaa cgctttcacg
     4141 acctcaggat gtttctcggc aaaatctttg cgcaccaccc agacgtccag cgttggcgca
     4201 ccccactgcc cgacctgttc agaatcggtc aacaccttgc cgtctttttc cagggcgtta
     4261 accgccggtg cccagacata agcaccatca atatctcccc gctgccaggc agcgataatc
     4321 gcgggcggct gcaggttcac aatctccact tgcccgggtt taatgcccca gtgtttcagc
     4381 gccgccagca ggctgtagtg ggtggtagag ataaacggta cggctatgcg tttgccaatc
     4441 agatcttccg gtttgctgat agttttcttt accaccagcg cttcggagtt acccagtttt
     4501 gacgccagca agaaaacttc aatcggcacc tgttggctgg ctgcaaccgc taacgggctg
     4561 gaaccgaggt tgccgatttg cacatcgcca gaagccagcg cccgcacgat gctggctccg
     4621 ctgtcaaact tacgccagtc cacggttgct ccgctttctt tagcaaaggt gttgtcggcc
     4681 tgagccactt tcgccggttc ggctgaggtt tgatacgcca cggtgacgtt caccgcctgt
     4741 gcctgaaaag cgatgaatgc cagtgcggca agaagtgtgt tacgcgatga aattgccatg
     4801 attgtctgct cccctgtctt gttatgggag cagtattcag gaataaaaac attcattaaa
     4861 agaattagtc gttatcgcac agatgatttt attcttagca aaaaaacggc taaggaaaga
     4921 ttaaatatg
//
LOCUS       DAZGVQ010000260         4921 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000260 DAZGVQ010000000
VERSION     DAZGVQ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.263"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            505..2625
                     /locus_tag="ACHLAG_005029"
                     /pseudo
     CDS             505..2625
                     /locus_tag="ACHLAG_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            4141..4419
                     /locus_tag="ACHLAG_005030"
                     /pseudo
     CDS             4141..4419
                     /locus_tag="ACHLAG_005030"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
     gene            4533..>4921
                     /locus_tag="ACHLAG_005031"
     CDS             4533..>4921
                     /locus_tag="ACHLAG_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="receptor-recognizing protein"
                     /protein_id="HFP0658103.1"
                     /translation="MTGQRWMSAARTAVQLPAAGSMSQMAGRSKEVQYSIGANHNYNK
                     DTLINYLKSQGATPVVVTITGDLVSSSSGVPCLDFPSSLTNSYISLVINAGVTVYGRG
                     GNGGSNAAGAAGGNAINNGIGTRLRIT"
BASE COUNT         1294 a         1171 c         1371 g         1085 t
ORIGIN      
        1 gggcaaggat agcccgccaa atcaaggagc atattcaaca tgtggtacag ggaaggtact
       61 atcacattta cacagggtag caatactctg gttggggcag ggacagcctg gaacgtaaca
      121 gctaatggtg tgttgccggg gatgatcgtc attggccccg acaataagct gtatgagatc
      181 aagcgcgtaa ccagtgatac gaacattgtt ctctcagaac cttataccgg cgaaactcag
      241 tctgaagttc cgtgccgaat cattacgacc tatgaaggcg acttaacaca gtttagcgcg
      301 cgctttacag cactaatgtc gcgtatgtcg gctgattcca agtctatgcg cagttggttg
      361 actgctctgg atgaggtgac aatcgagcgt gaagacggta cagaagtaac cgttaagccg
      421 ctaatgcaga tcgtcaacga gcacaacgaa aacgttgagt ggtataaaaa taacactgac
      481 gcgattgatg ctgctggcga caaagctcgt gaggcggcgg ccagtgctgc cgcagcagca
      541 gaaagcgcca ataccgctgg agaaaaagcc tctcaagcgt ctcaaagtgc atccgctgcg
      601 gcatcctcac aaagtgccgc aagtgcgagt gcaactgctg cgaaaaaatc tgaaacgaat
      661 gccgcagcgt cacaacaatc agcagccact tctgcatcca ccgcgaccac gaaagcgtca
      721 gaagctgcca cctcagcccg ggatgcggcg gcctcaaaag aggcagcgaa atcatcagaa
      781 acgaacgcat ccttgagcgc cagtagcgca gcttcctcgg caacggcggc aggaaattcc
      841 gcgaaggcgg caaaaacgtc cgagacgaac gccaggtctt ctgagacggc agcgggacag
      901 agcgcctcag ctgcggcagg ctcaaaaaca gcggctgcgt cgtctgccag tgccgcgtca
      961 acaagtgccg ggcaggcctc agccagtgcc accgccgccg gaaaatcggc agaaagtgcc
     1021 gcatcgtctg cttcaacagc cacaacgaag gctggcgaag ccactgaaca ggccagcgca
     1081 gcagcgaggt ctgcttccgc agcgaagaca tccgaaacga acgcgaaagc gtcggaaacc
     1141 agcgcagaat cctcaaaaac ggctgccgca tcgtccgcca gttcggcggc gtcatcggca
     1201 tcatctgcgt ctgcttcaaa agatgaggcg accagacagg cgtcagcagc gaggggtagc
     1261 gccacgacgg catccacgaa ggcgacagag gctgctggca gtgcggcagc ggcagcacag
     1321 agcaaaagta cggcggaatc cgcggcaacg cgcgccgaga cagcagcaaa acgggcagag
     1381 gatattgcat ccgccgtggc gcttgaggat gcgagcacga cgaaaaaggg gatagtacag
     1441 ctcagcagtg cgaccaacag cacttccgag tcacaggcgg caacgccaaa agccgttaag
     1501 gccgcgtgtg acctggctaa cgggaaatac accgcacagg acgctacgac agcacaaaaa
     1561 ggaattgtcc agctcagtag tgcaaccaac agcacatctg aaacgcttgc tgcgacaccg
     1621 aaagcagtga aagcagctaa tgacaatgcg aatggtcggg taccttctgc ccgtaaggtg
     1681 aatggtaagg cgctttcagc ggatataaca ctgacgccga aagatattgg tacgcttaac
     1741 tcaacaacaa tgtcattcag cggtggtgct ggttggttca aattagcaac ggtaaccatg
     1801 ccacaggcga gttctgttgt ttcaattacg ttgattggtg gcgctggatt taacgtgggg
     1861 tcacctcaac aggcaggtat atctgaactt gttttacgtg caggtaatgg taatccgaag
     1921 gggattacag gtgctttgtg gaagcgtaca gctgtcggat taacgaattt cgcctggatc
     1981 aacacatccg gcgatacata tgatatttac gttgaaattg gcaattatgc gacgagtgta
     2041 aatatccatt gggattgtac tgcaaatgcg acagtttcta tttatacctc gccaacatat
     2101 tcagcgagta agccttccag cgttaccgat ggtgttgttt atacgatgta tagcacacat
     2161 cagaaaccga cgccgttaga tattggagca ctgccaacaa ccggaggaac agtttcaggt
     2221 ccgttgtctg ttacaggtgg attaactggt tcattgaatg gtaatgcaag tacagccacg
     2281 aaattgcaga cggcaagatc tatcggtgga gttgttttcg acggttctgc aaatattaac
     2341 cttccaggtg taaacactac gggtaatcag aacaccactg gtaatgctgc aactgctaca
     2401 aaacttcaga tggcaagaac tatcggcggc gtgagctttg atggtactgc gaacataaat
     2461 ttgcctggtg taaacacggc aggtaatcag aatacaacgg gcaacgctgc tactgctacg
     2521 aagttgcaaa cagcgagaaa catcaatggt gttaagtttg atggctcagg cgatatcaac
     2581 attaatacac tggtatctcg tggccgagtt acggcgttaa gtggctctac tcagggtact
     2641 gctggcattc aaatgtacga agcctacaac aatggctacc cgaccatgta tggcaacgta
     2701 ttgcacatga aaggtgcgag tgcttctggt gagggcgaga tgcttgttgg ctggagcgga
     2761 acggatggtg ctcatgcacc ggtttatgtt cgttcacgcc gtgatacttc gacagcgaat
     2821 tggtctggtt gggcgcaggt ctataccact gctcataaac ccacagcgaa agatgttggg
     2881 gctgcacaga cgtttagcgc gtcatacagt actggggctg gcaactggac taccgctgaa
     2941 tttatcgcat ggctgaagga gcgtggcgct ttcgaagtgc cgtactggat gatgaagggt
     3001 tcctggtcat atgccgacaa taaaattatc actgatacag gtgtcggcaa tatctgtctt
     3061 gctggtgcgg ttatcgaggt tttaggtacc gaaggcgcaa tgaccatccg agttactacg
     3121 cctacaacga cgaccggtgg tgggattgcc tgtgctcagt tcacctatat taatcacggt
     3181 agcgcgtatg cccctgcctg gcgcagagat tacaacacga ctctcaaacc aactgcggct
     3241 gatgtcggtg cgttaccgat ttctggcgga acaatgactg gtgtgctgac gctgcaaaat
     3301 gtgagtcagc ccctcaagac gcaaggtgga ggcattctgg caaatgatgg aaatttatac
     3361 attaataagt cgggatttgc aggctggatt gatgcgctat ttatgaaaaa cagcggcggt
     3421 acgatgtccg gacagcttaa gatccgctct acggatgggt tacgcattta cgatgcggca
     3481 tacgggatga tttttcgccg ttccgaaaat aatttttacc ttatcccgac agcaaaagac
     3541 cagggagaaa atggtgatat aggttcgctt cgtccatttt atgtaaatct cactaatggc
     3601 agagtagcaa tgggcaatgg tgctgttgtt gacggtggtc ttgggctggg tgtggtcaat
     3661 ggtcttggcg gtaattccat tgttctgggt gacaatgaca ccggctttaa acagaacgga
     3721 gatggcattc tggatgttta cgccaatagc gcccatgttt ttaggttcgt taatagcacg
     3781 ctgcaaagcc ttaagccgtt gagtgtaacg ggtgacatcg cgtcaagtgc gtgggtttac
     3841 gccaaccgtt tttccatcaa cagcggctct ggcgcgtgga tagatatgcg taaccagaac
     3901 gtcatcttcg gggggaacgc tgtaagtaca aactctgcac aagcgttgtt gagacaagac
     3961 cacgcggatc gcaaattctt cttgggtggc ttgggaaaca gtcagtttgg tttttacatg
     4021 atcaacaact cacgtaccgc aaacggcact gacgctgccg cttatctcca gaacgacggc
     4081 acttgggttt gcgcaggcaa cggtagtttt aatgacgttt acatccgttc cgaccgccgt
     4141 agtaagcgca atattcgcaa aattgagcgg gcgctcgaca agctggagca gatcgaaggt
     4201 gttctctacg agattcaggt ctgtggccgt tatgagcagt ctggcggcct catcgctcag
     4261 gacgtccaga atgttcagcc tgaactggta acggttgacc ataacgatca gtcaggagag
     4321 cctcgtctgc gtcttaacta caacggcgtt atcggcatgt tggtcgaggc tgtcaaagag
     4381 ctgcgcgagg aagtgcgcga actgaaggca aaaatgtaat caaggtgaat ggtgaggggc
     4441 caccctcacc attcaggata ttcacatcaa aaaggagatt agatgtgcca attgtcggtg
     4501 ttcccggttg gattgggtct tcggctgtca gtgtgacagg ccagagatgg atgagtgcgg
     4561 ctcgaacggc ggtgcaactg ccagcggctg gttcaatgtc tcaaatggcc gggcgatcta
     4621 aagaagttca gtacagcatc ggtgcgaatc ataactacaa caaagacaca ctgatcaact
     4681 atttaaaatc ccagggggcg acgccagtcg ttgttaccat tacaggggat ctggtgtcgt
     4741 ccagtagcgg cgtgccatgt ttagatttcc cgagttcact taccaactct tatatcagcc
     4801 ttgtaatcaa cgcaggtgtg actgtctatg gacgcggtgg caatggtggt agtaacgctg
     4861 ccggtgctgc tggtggtaac gcaattaata atggcatcgg tacacgcttg cgtattacga
     4921 a
//
LOCUS       DAZGVQ010000261         4873 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000261 DAZGVQ010000000
VERSION     DAZGVQ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.264"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            106..873
                     /locus_tag="ACHLAG_005032"
     CDS             106..873
                     /locus_tag="ACHLAG_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658104.1"
                     /translation="MDDLSVYARSLDKAKYYVYCLYDTEDKMKRPFYIGKGKSTRCLD
                     HIKYPDDSPKSMRIKELLANKKLGIDILRHGMDETTAKLVEATCIDLMGVGELTNKVR
                     GSGSMMGRISLDAYHHLVLQEETEIAPEHAGLAFLLNSTYKSGMSALALYEATRGVWA
                     KVPRDENLKYAYATYGGLIMEVYQIECWVKAGSQQYFTRDIALGPDTKRYEFVGRIAD
                     EHIRKLYVGKLIKKPPSYGSPFVKVGVVRTEHSVSAA"
     gene            complement(926..1279)
                     /locus_tag="ACHLAG_005033"
     CDS             complement(926..1279)
                     /locus_tag="ACHLAG_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658105.1"
                     /translation="MKMGDLAKRKEPDAPAKNTTPLRQPVRPQGRPTRGKEKIKSRTM
                     SLEDEYFELLEMMKFIPRFEKFTRSDVIRAAIFHLAEKSPQEIEDIVKLNEAITAADV
                     TMRTDEIKRELMKKG"
     gene            complement(1285..1953)
                     /locus_tag="ACHLAG_005034"
     CDS             complement(1285..1953)
                     /locus_tag="ACHLAG_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0658106.1"
                     /translation="MTKIFVVGGTKGGPGKSTVAQQIAVCLKVKKKKKVYITDIDIQR
                     TTTSWCEDRRQNEDLELIPFAYVQDDIIKHLKSLQGRAEFVVVDAGGFDSEIQRQAML
                     MADVIIIPLRPKRRDLKSLRDIDPIIDNVRNVNDKVKVRAVMNQCPALPSQVSRILAA
                     KEIVETFGIESAPVNLYNRNVYDDAEESGRSIFEMTGSERDKKAEAEFEEFVDYLLSL
                     EEEE"
     gene            2272..2541
                     /locus_tag="ACHLAG_005035"
     CDS             2272..2541
                     /locus_tag="ACHLAG_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004109769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFP0658107.1"
                     /translation="MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERA
                     VKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKRAADRLKSME"
     gene            2549..3070
                     /locus_tag="ACHLAG_005036"
     CDS             2549..3070
                     /locus_tag="ACHLAG_005036"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0658108.1"
                     /translation="MSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKIL
                     RAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQKKVPYRNVPSVTLGRLALDKSL
                     QGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
                     FYPTKSIEKLFEE"
     gene            complement(3239..3490)
                     /locus_tag="ACHLAG_005037"
     CDS             complement(3239..3490)
                     /locus_tag="ACHLAG_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658109.1"
                     /translation="MLGSLMTKLKVALITLAAVLFVLVGAYTMGGNAARRAMEEKAKQ
                     EDRKRLQNTVKVANETSSKIRQKDASAVHRELYDKWVRH"
     gene            complement(3492..4184)
                     /locus_tag="ACHLAG_005038"
     CDS             complement(3492..4184)
                     /locus_tag="ACHLAG_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658110.1"
                     /translation="MKTPRGIRNNNPGNLDKGSPWQGLIDNPAEPRFCTFKDPVWGIR
                     ALAVTLITYHDKRRAKDGTSIDTIREVIERWAPPNENNTDAYINEVSKAVGVTADMII
                     DLHDYDILRPLVEAIIRHENGRGPLKTLNTWYAAEVIEEGLRRAGVVKPVKTVKAVPV
                     TKETAGATVTAGIGLAQLADVMPQVSAAMDKAQGHISSGDTVRIIFGIATIVVAGFIA
                     WSQVRKHQKGMV"
     gene            complement(4198..4521)
                     /locus_tag="ACHLAG_005039"
     CDS             complement(4198..4521)
                     /locus_tag="ACHLAG_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658111.1"
                     /translation="MSNEMAGVTPEQVERIAAIVAREVVGKLGKELREEIGQEVNDQL
                     KAYFGDMTPSQHSIQHSNLDKLLNRLDAISSGFFGGIISKITSFLITALLLGLAAYGV
                     KNGLQ"
     gene            complement(4596..>4873)
                     /locus_tag="ACHLAG_005040"
     CDS             complement(4596..>4873)
                     /locus_tag="ACHLAG_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="receptor-recognizing protein"
                     /protein_id="HFP0658112.1"
                     /translation="GGGCPFGAGGSSSHMSSGATAGTISAPGKGSVGEGSLSAYTGGS
                     GGNVGAAGGRCNTQGNGTEYNGGAAGKAVTGNAPTWTKVGAIYGAHV"
BASE COUNT         1293 a         1157 c         1095 g         1328 t
ORIGIN      
        1 ttgtcgtctt agacgcccag aagctctaca ctttgagcct gttaaattga ttaaaaactt
       61 atgtgtaaaa tagaacgcat tagtcagtag caaagagatg caataatgga cgatttatcg
      121 gtatacgcga gatcattgga taaggctaaa tactatgttt actgtctcta tgatacagaa
      181 gacaagatga aaagaccgtt ttacatagga aaaggcaaat cgactcgttg tctcgatcac
      241 atcaagtatc ccgatgactc tcccaaatcc atgcggatca aagaactttt ggctaataaa
      301 aaattaggca tagatatact tcgtcatggt atggatgaaa ccacagccaa acttgttgag
      361 gcgacatgca tcgaccttat gggggtcgga gagcttacga ataaggtacg aggaagtggt
      421 tccatgatgg gcagaatatc actggacgcc taccatcact tagtgctgca agaggaaact
      481 gaaattgctc ctgagcacgc cggtcttgcc ttcctgctaa acagcaccta caagtcagga
      541 atgtccgctc tggcattata tgaagctacg cgtggcgtat gggcaaaggt tccaagagac
      601 gagaatctca aatatgccta cgctacatat ggtgggctga taatggaagt ttatcagatt
      661 gaatgctggg tcaaagccgg ttctcagcag tactttacaa gggatatagc tcttggcccc
      721 gacacaaaac gttatgaatt tgttggcaga atagccgatg agcatattag aaaactgtat
      781 gtaggcaaat taatcaagaa gccaccaagc tacggtagcc cttttgtgaa ggttggggtg
      841 gttaggactg aacatagtgt tagcgcggca taaagctgac actgtagtta tgcattggcg
      901 caatgtacgc gccaatgcat gatttttaac ctttcttcat caactctcgt ttgatttcat
      961 cggtacgcat cgtgacatcg gcagcggtga tcgcctcatt caatttcacg atgtcctcga
     1021 tttcctgcgg tgacttctct gccagatgga aaatggctgc tcgaatcacg tcagaacgag
     1081 tgaacttctc gaagcgaggg atgaacttca tcatctccag cagttcgaag tattcgtcct
     1141 ccagtgacat tgtgcggctt ttaattttct ctttgccacg agtcgggcgt ccctgtggtc
     1201 tgactggttg gcgcaaagga gttgtgttct tagccggtgc atcaggctct ttgcgctttg
     1261 ctaggtcacc cattttcatg gacattattc ttcttcctcc agactcaaca gataatctac
     1321 aaattcttca aactcggctt ccgccttttt gtcgcgctcg ctaccggtca tttcaaagat
     1381 agaacgacca gactcttccg catcatcata gacgttgcgg ttatacagat tgactggcgc
     1441 agactcgatt ccaaacgtct caacaatctc tttagccgcc agaatgcgag acacttgtga
     1501 tggcaaagcc gggcactggt tcatgaccgc gcggaccttc actttatcgt ttacattgcg
     1561 aacattgtcg ataataggat cgatgtcacg taaagatttc aaatcacgac gcttaggacg
     1621 cagcgggata atgataacgt cagccatcag catcgcttgt cgctgaattt cggagtcgaa
     1681 gccaccagca tctaccacta caaactcagc tctaccctga agcgatttta ggtgcttaat
     1741 gatgtcatcc tgaacgtatg caaaaggaat cagctcaagg tcttcgttct gtcgacggtc
     1801 ttcacaccag ctcgttgtcg tgcgctgaat atctatatcg gtaatataaa ccttcttctt
     1861 ctttttgact ttcaggcaaa cggcaatttg ctgggcaacg gtggatttgc caggcccgcc
     1921 ctttgtgccg ccaaccacaa agatcttggt cattggagag ttccctttgc gtatataatt
     1981 atcgtctgaa acaacttgtt ttcttatatg tgatatagcc taaatgccta cggctgcggt
     2041 gtaaaggtta aatggtaggt tgtgattaac aattggcaat catagcttgt atttgtctgt
     2101 tgaaaaaagt aacaatgacc tctatattac aagtacggtg atatgccgta cacaatttga
     2161 taatgcacac gtaatgggct atagcctttt aaagtaattt ttgagtcgaa aactcagttt
     2221 gaacagagga gattgatggc cttcctacat cacggccatc aggaggaata catgtcagca
     2281 ctaaaaaagc agcgcatcga tttgagatta accgatgacg ataaaagcat catcgaggaa
     2341 gctgccgcaa tgtctaacca gagcattact cagtttatgg ttagcagtgc atctgaacgt
     2401 gccgtgaaag ttatagagca acaccgtaga ctagttctga atgaagaatc ctggaatctg
     2461 gttatggacg ctataagcaa tcctccggca ccgaacgaca agctgaaacg agctgctgat
     2521 cgtctgaaaa gcatggagta gtttactcgt gagcaataca acgatagaga ttttctctgg
     2581 agagaaagat tatgatctaa acggttttga ttgcggcgaa gagtcactaa acgccttttt
     2641 agccaaccac ttaaaaaggc agcatgaggg aaaaattctt cgtgcttatg tgctttgcac
     2701 tcaagaagaa aggccaaaag tgttaggata ttacactttg tcaggtagtt gttttgaaaa
     2761 ggagtcctta ccttcaagaa gcaaacaaaa gaaggttcct tatcggaatg ttccaagtgt
     2821 tactttgggt aggctggctt tggacaagtc ccttcaaggt caaggatttg gctcaatgct
     2881 tgtaacacat gcaatgcgcg ttgtgtacaa tgcatctctt gctgtaggca ttcatggact
     2941 tttcgttgag gcgttaaatg acaaagccaa ggcgttttat aaaagtttag gctttatcca
     3001 gctggttggt aacaacgagc gttctttgtt ctatcctaca aaatctatcg aaaaattgtt
     3061 cgaagaataa tgtgttcccc tcatttgagg ggaacttcga catcaccaac cacaaaccct
     3121 ctctcccatc gtattgtgaa aaaggatttg gcgttcggtt tcttcagtca ttacatcatt
     3181 attgctgata tagataggct cagccccatc gcagaacaac acgctggctg tttgtggttt
     3241 aatgacgcac ccacttatca tacaactcac gatgaacggc agaagcatct ttctgacgta
     3301 ttttactgct cgtctcattc gccactttca ctgtgttttg aagtcgtttt ctgtcttcct
     3361 gttttgcctt ctcttccatt gctcgtcgcg ccgcattccc gcccatcgtg taagcgccga
     3421 caagaacgaa aagaacggca gccagcgtaa tcaaagcaac ttttagcttt gtcatcaggc
     3481 tgcctagcat attagaccat ccctttctgg tgttttctta cctgcgacca ggcaatgaat
     3541 cctgccacaa caatagtggc aataccgaag atgatgcgta ctgtatcccc gctagagata
     3601 tgaccttgtg ctttatccat agcagcggaa acctgcggca taacatcggc cagctgcgcc
     3661 agaccaatac ctgctgtaac agttgcgcct gcggtttctt tagttacagg aacagccttc
     3721 acggttttca ccggcttaac aacgccagct cgacgcagac cttcctcaat aacttctgcc
     3781 gcataccagg tgttcagcgt ttttagcggg cctcggccat tctcgtggcg aatgattgcc
     3841 tcaaccaaag gtcgaaggat gtcgtaatca tgcagatcga tgatcatgtc tgcggttaca
     3901 ccaacggctt tagacacctc attaatgtag gcgtcagtat tgttttcatt cggcggtgcc
     3961 cagcgttcaa taacttcacg aatggtatcg atacttgtgc cgtcttttgc gcgacgttta
     4021 tcgtggtagg taattagagt caccgccagc gcacgaatcc cccaaacagg gtctttaaac
     4081 gtgcaaaagc gcggttcggc agggttgtcg attagcccct gccacggcga acctttgtcg
     4141 aggttgccag ggttgttatt acgaatgcct ctcggagttt tcatccttga tctcctgtta
     4201 ttgcagtcca ttcttcacgc cataagcggc cagacccagc agcagcgcgg taatcaggaa
     4261 cgacgttatc tttgagataa tgccgccaaa gaacccgctg gagatggcgt ctagccgatt
     4321 aagtagtttg tccaggttgg agtgctgaat gctgtgttgc gaaggcgtca tatcgccaaa
     4381 gtaggctttg agctggtcgt tgacctcctg gccaatttcc tcacgcaact ccttacctaa
     4441 tttgccgaca acttcacgcg caacgatagc ggcaatgcgc tctacctgct ctggcgttac
     4501 gcctgccatc tcgttcgaca ttatttcctc catgaatcgt caaatcggga tggcggtttt
     4561 atatcataaa acgcccacac ttggtaggta agtacttaca catgagcgcc gtaaattgcc
     4621 ccaactttag tccaagttgg agcattacca gtaacagctt taccagctgc tccgccgtta
     4681 tattctgtac cgttaccttg agtattacat cttcctccag cagcaccgac attaccaccc
     4741 gaaccgcctg tatatgcact aagagacccc tcaccaacag atcctttacc aggagcggaa
     4801 atagtaccag cagttgcacc ggaactcata tgagaggaag acccaccagc accgaatggg
     4861 caaccaccgc cac
//
LOCUS       DAZGVQ010000262         4729 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000262 DAZGVQ010000000
VERSION     DAZGVQ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.265"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            51..851
                     /gene="tcyJ"
                     /locus_tag="ACHLAG_005041"
     CDS             51..851
                     /gene="tcyJ"
                     /locus_tag="ACHLAG_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP0658113.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            956..1942
                     /gene="dcyD"
                     /locus_tag="ACHLAG_005042"
     CDS             956..1942
                     /gene="dcyD"
                     /locus_tag="ACHLAG_005042"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFP0658114.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            1957..2625
                     /gene="tcyL"
                     /locus_tag="ACHLAG_005043"
     CDS             1957..2625
                     /gene="tcyL"
                     /locus_tag="ACHLAG_005043"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFP0658115.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSLIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            2622..3374
                     /gene="tcyN"
                     /locus_tag="ACHLAG_005044"
     CDS             2622..3374
                     /gene="tcyN"
                     /locus_tag="ACHLAG_005044"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFP0658116.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            3604..4326
                     /gene="sdiA"
                     /locus_tag="ACHLAG_005045"
     CDS             3604..4326
                     /gene="sdiA"
                     /locus_tag="ACHLAG_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFP0658117.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(4393..4617)
                     /gene="yecF"
                     /locus_tag="ACHLAG_005046"
     CDS             complement(4393..4617)
                     /gene="yecF"
                     /locus_tag="ACHLAG_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFP0658118.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(4604..>4729)
                     /gene="yecU"
                     /locus_tag="ACHLAG_005047"
     CDS             complement(4604..>4729)
                     /gene="yecU"
                     /locus_tag="ACHLAG_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFP0658119.1"
                     /translation="DITLKKQPLLFLAKLMVYSAALTFFTANFHCNMTRKINEYA"
BASE COUNT         1180 a         1098 c         1274 g         1177 t
ORIGIN      
        1 caaacaatgg ctctacactg caaacagaca taacaacatt cggggtaaat atgaaattag
       61 cacatctggg acgtcaggca ttgatgggtg tgatggccgt agcgctggtt gcgggcatga
      121 gcgttaaaag ttttgcagat gaaggtctgc ttaataaagt taaagagcgc ggcacgctgc
      181 tggtagggct ggaaggcact tatccgccgt tcagttttca gggagatgac ggcaaattaa
      241 ccggttttga agtggaattt gcccaacagc tggcaaaaca tcttggcgtt gaggcgtcac
      301 taaaaccgac caaatgggac ggtatgctgg cgtcgctgga ctctaaacgc attgatgtgg
      361 tgattaatca ggtcaccatt tctgacgagc gcaagaaaaa atacgatttc tctacccctt
      421 acaccatttc cggtattcag gcgctggtga aaaaaggtaa cgaaggcacc attaaaacgg
      481 ctgctgatct gaaaggcaaa aaagtgggtg tcggtctggg caccaactat gaagagtggc
      541 tgcggcagaa tgttcagggc gtcgatgtgc gtacctatga tgatgacccg accaaatatc
      601 aggatctgcg cgtaggccgt atcgatgcga tcctcgttga tcgtctggcg gcgctggatc
      661 tggtgaagaa aaccaacgat acgctggcag taaccggtga agcattctcc cgtcaggagt
      721 ctggcgtggc gctgcgtaaa ggaaatgaag acctgctgaa agcggtgaat gatgcaattg
      781 cggaaatgca aaaagacggc actctgcaag ctctttccga aaaatggttt ggtgctgatg
      841 tgaccaaata atcagcataa tgacaaaaaa gggcgctttc actagcgcct tttttattta
      901 cgcgttttca gcgtgcataa taagaaaatc acaacaacac acatcggagg ctgttatgcc
      961 actgcataat ttaacccgtt ttccacggtt ggagtttatc ggcgcgccaa cgccgctcga
     1021 atatctgccg cgcttttctg attatcttgg acgggaaatt ttcatcaaac gggatgacgt
     1081 cacacccatg gcaatgggcg gcaataaatt acgtaagctg gaatttctcg cggcagatgc
     1141 cctgcgcgaa ggtgccgata cgctgattac tgccggcgcg atccagtcta accatgtgcg
     1201 ccagactgcc gcagtcgcgg cgaaactcgg tctgcactgc gtggcgctgc tggaaaatcc
     1261 tattggcaca accgcagaaa actatttaac caacggcaat cgtttgttgc tggatctgtt
     1321 caatacccag attgaaatgt gcgacgcact gaccgatccc aatgcccaac tggaagagct
     1381 ggcgacgcga gtcgaagcac aaggctttcg cccgtatgtc attccggttg gcggttctaa
     1441 tgctctgggc gcgctgggtt atgtggagag tgcgctggaa atcgcgcaac agtgtgaagg
     1501 ggcggttaat atttcgtcgg tggtggtcgc atcgggcagt gccggaactc acgccggatt
     1561 ggctgttggg ctggaacacc tgatgcctga aagcgaactg attggcgtga ccgtgtcgcg
     1621 ttccgttgcc gatcaattgc cgaaagtggt taacctacaa caggcgattg cgaaagaact
     1681 ggagctgacc gcatcagcgg aaattttact ctgggatgac tattttgcac ctggctacgg
     1741 cgtgccgaac gacgaaggca tggaagcagt gaaattgctg gcgcgtctgg aaggcattct
     1801 gcttgatcct gtgtataccg gaaaagcgat ggcggggctg attgacggta tcagtcagaa
     1861 acgcttcaaa gatgaagggc cgattctgtt tattcatacc ggcggcgcgc ctgcgctgtt
     1921 cgcctatcat ccccacgttt agctccggga acgctcatgc aagaaagtat acaactggtt
     1981 attgattcct tgccgttcct gttgaaaggg gctgggtata ccctgcaact cagtatcggc
     2041 ggcatgtttt ttggcttact gctagggttt attctcgcgc tgatgcgtct gtcgctaatc
     2101 tggcccgtgc gctggctggc gcgtttttat atctctattt ttcgtggtac accgctcatc
     2161 gcacaactgt ttatgatcta ttacggcctg ccgcagtttg gtatcgaatt agatcccatt
     2221 ccgtcagcaa tgattggtct gtcgctgaac actgccgcct atgctgccga aacgctgcgc
     2281 gccgccattt cttctattga taaaggtcag tgggaagccg ctgccagtat tggtatgacg
     2341 ccgtggcaga ccatgcgtcg cgcgattttg ccacaggcgg cgcgggtggc gttgccgccg
     2401 ctgtcgaaca gctttatcag cctggtaaaa gatacgtcgc tggccgcgac aatccaggtg
     2461 ccggagctgt tccgtcaagc gcagttgatt acctcgcgca cgctggaggt gttcaccatg
     2521 tacctggcgg cttcgctcat ctactggatc atggcgacag tattatcgac gttgcagaac
     2581 cattttgaga atcaacttaa tcgccaggag agagaaccaa aatgagtgcc attgaagtta
     2641 agaacctggt gaaaaaattt cacggtcaga cggtgctgca cggtatcgac ctcgaggtaa
     2701 agcctggcga agtggtggca attatcggtc cgagtggttc cggcaaaacc acgttgctac
     2761 gcagcataaa tctgctggaa caaccggaag cgggaacgat caccgttggc gatatcacta
     2821 ttgatactgc acgttcatta agtcagcaaa aatcgctgat tcgccagttg cgtcagcacg
     2881 tcgggtttgt cttccagaac tttaatttgt ttccgcatcg tacggtgctg gaaaacatta
     2941 ttgaagggcc ggtgatcgtc aaaggcgaac cgaaagaaga ggccacggcg cgcgctcgcg
     3001 agctgctggc gaaagttggg ctggccggta aagaaaccag ctatccacgt cgtttgtctg
     3061 gcggtcaaca gcagcgtgtt gcgattgcgc gtgcgctggc aatgcgtcct gaggtgattt
     3121 tgtttgacga gccaacgtca gcgctggatc cagagctggt gggcgaagtc ctgaacacaa
     3181 tccgccagct ggcgcaggaa aagcgcacga tggtgattgt gacgcacgaa atgagctttg
     3241 cccgggatgt tgcggaccgg gcgattttta tggaccaggg gcggatagtc gagcaggggg
     3301 cagcaaaagc gttatttgct gatccccagc agcctcgcac ccgccagttc ctcgagaagt
     3361 ttctgctgca ataataagaa aaaatcagcc ccgacgattc acctgtcggg gcgggacgcc
     3421 atttcaagcg tgataaaact gcttaacaaa tcagcataac tcattaataa cataagagaa
     3481 tgcgatggct tgcaaaagta attcattgcc tgaataatat aaattatata taaatcttat
     3541 ttatgtgata gtttgaatta tcattataaa tgatactcac tctcaggggc gttgcggttt
     3601 actatgcagg atacggattt tttcagctgg cgtcgcacga tgctgttgcg ttttcagagg
     3661 atggaggccg cagaagaggt ctaccatgaa atagagcttc aggctcagca gctggagtac
     3721 gattactatt cgttatgtgt tcgccacccg gtaccattca cgcgacctaa agtggctttc
     3781 tacaccaatt accctgaggc gtgggttagt tattatcagg caaaaaactt tctcgcaatt
     3841 gatccggtgc tgaaccctga aaactttagt cagggccatt taatgtggaa tgatgactta
     3901 ttcagcgaag cacagccgtt atgggaagcc gcgcgcgcac atggtttacg ccgcggtgtc
     3961 actcagtatt taatgctgcc aaatcgggcg ctgggctttt tgtccttttc ccgttgcagc
     4021 acgcgcgaaa tacccattct tagtgatgaa ctgcaattaa aaatgcagtt actggtgcgc
     4081 gaaagtctga tggctctgat gcgtttaaat gatgaaatag tgatgacgcc agagatgaat
     4141 ttcagcaagc gcgaaaaaga aattctgaaa tggacggcgg aagggaagac atcagcagag
     4201 atagcgatga ttttgtcaat ctctgagaat acggtcaatt tccatcagaa aaacatgcag
     4261 aaaaaaatca atgcaccaaa taagacccag gtggcctgtt acgcggccgc tactggctta
     4321 atttgatcgc ttttctttct gcgtgccaga tgcaaaaacc ggctgaaagg cacgctctca
     4381 gccggtttta tattactgac ggtaggcttg tttaatttgc ttaaccgttt tggaaaatac
     4441 tgcagcctgg gtttcgtctt cgatcagcgc aagctgtttt tccatcttta acatcacgcg
     4501 gcccgcgtca gcttgtccca tcgcctgcag catcagcgtc agcatcgctt tcaggcagga
     4561 gacttcattt gccagttctt gattattctc ggcagtagaa aaatcaggcg tactcattta
     4621 ttttcctcgt catgttgcaa tgaaaatttg cggtgaaaaa tgttaaggcg gcggagtata
     4681 ccataagctt tgctaaaaat agcagtggtt gttttttgag cgtgatatc
//
LOCUS       DAZGVQ010000263         4676 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000263 DAZGVQ010000000
VERSION     DAZGVQ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.266"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..618
                     /gene="hprR"
                     /locus_tag="ACHLAG_005048"
                     /pseudo
     CDS             1..618
                     /gene="hprR"
                     /locus_tag="ACHLAG_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310734.3"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
     gene            618..1976
                     /gene="hprS"
                     /locus_tag="ACHLAG_005049"
     CDS             618..1976
                     /gene="hprS"
                     /locus_tag="ACHLAG_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFP0658120.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDGMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALIKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(2084..2935)
                     /gene="hchA"
                     /locus_tag="ACHLAG_005050"
     CDS             complement(2084..2935)
                     /gene="hchA"
                     /locus_tag="ACHLAG_005050"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFP0658121.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMKIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYVD"
     gene            complement(3527..4675)
                     /gene="ompC"
                     /locus_tag="ACHLAG_005051"
     CDS             complement(3527..4675)
                     /gene="ompC"
                     /locus_tag="ACHLAG_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP0658122.1"
                     /translation="MAMLVPALLVAGAANAAEIYNKNGNKLDLYGKVDARHTFSDNPG
                     DDGDETIIELGFKGETQITDQLTGYGQALTKTKASDTEGSDNTYVKLAFAGLKFGEMG
                     SFDYGRNYGVIYDVEAWTDMLPVFGGDSYTWTDNFMAGRANGVATYRNSDFFGLVEGL
                     NFALQYQGNNEGSNAGEDQEGTKNGHEDVRFQNGDGFGLSTSYDFDFGLSLGAAYSNS
                     DRTNSQVALGGYHYNEYSKFAGGDTAEAWTFGAKYDANNVYLAMMYAETRNMTPYGNV
                     GIANKTQNFEAVAQYQFDFGLRPSLAYVYSKGKDLGGNDYNNNGHQEYVDQDLVNYVE
                     IGATYYFNKNFSTYVDYKINLLDKDDDFYDNNGIATDDVVGVGLVYQF"
BASE COUNT         1315 a          948 c         1080 g         1333 t
ORIGIN      
        1 gggctttccg aagcgggtta tgtcattgat gccgtttctg atggcagaga tgggctttat
       61 cttgcgctga aggatgatta tgcattgatc attctggata ttatgcttcc gggtatggat
      121 ggctggcaga tcttacaaac gttaagaaca gcaaagcaaa cccctgttat ttgccttact
      181 gcaagggatt ctgtcgatga cagagtcaga gggctggaca gtggagcaaa tgattatctg
      241 gtaaaacctt tttcattttc tgagttgctg gcaagggttc gggcacaatt aaggcaacat
      301 cacgctttga attcaacatt ataaatcagt ggcttaagaa tggactctgt aagtcaaagt
      361 gtgagcaggg acaatatcag tattacactg acgcgcaagg agtttcagtt actttggcta
      421 ctggcctcca gagctggcga aattataccc agaacggtta ttgcgagtga aatttgggga
      481 atcaactttg atagtgatac caatacggtg gatgtcgcca ttcgcaggct ccgcgcaaaa
      541 gttgatgatc cttttcctga aaagctaatc gccacaatcc gggggatggg ctattcattc
      601 gtagcggtaa aaaaataatg aaaagattat ctataaccgt ccgtttaacc ttgcttttta
      661 tattgctgct gtctgttgct ggcgctggaa ttgtctggac tctctataat ggcctggcaa
      721 gtgagttgaa atggcgcgat gatacaacac tcattaaccg gacagcacag atcaagcagt
      781 tgttaattga tggggtaaat ccagatacgt tacctgtgta ctttaaccgg atgatggatg
      841 ttagtcagga tatcttgatc attcatggtg atggcatcaa taaaattgtt aaccggacaa
      901 atgtcagtga tggcatgtta aataacatac ctgctagtga gacaatcagc gcagctggca
      961 tttacagaag cattattaat gacacagaga tagatgcttt acgaattaat attgatgaag
     1021 tttcgccatc attaacggtt actgtggcta aattggcttc agccagacat aacatgcttg
     1081 aacagtataa aattaatagc attataattt gcattgtcgc cattgtactt tgctcagtat
     1141 taagtccgct gttaatcaga acggggttac gagagatcaa aaagttgagt ggtgtaacgg
     1201 aagcgctgaa ttataacgat agccgggagc ctgttgaggt tagcgcatta ccgagagaac
     1261 taaaacctct tgggcaggcg ttgaataaaa tgcatcaagc cttaatcaaa gattttgagc
     1321 gactaagtca gtttgctgac gatctcgctc atgaacttag aacgccaatt aatgcattac
     1381 tgggtcagaa tcaggttacg ctcagtcaaa ccagaagtat cgctgaatat caaaaaacaa
     1441 ttgccggtaa cattgaagag ctggaaaata tttcgcggtt aacagagaac atactgtttc
     1501 ttgccagggc agataaaaac aatgttttgg tgaaactgga ctcgctttct ctcaataagg
     1561 aagtcgaaaa tttgttggac tatcttgaat acctttcaga cgagaaagag atttgcttta
     1621 aggtcgagtg caatcagcaa atctttgcgg ataaaatttt actgcaacga atgttatcga
     1681 atcttattgt taacgccatt cgatattcgc cagaaaaatc gcgcattcat ataaccagtt
     1741 ttcttgatac caacggctat cttaatattg atgtcgccag tcctggaacg aaaattcatg
     1801 agcctgaaaa actcttccgt agattttggc ggggagataa ttcgcgtcat tccgtaggtc
     1861 agggacttgg tctttcttta gtcaaagcga ttgccgaatt gcatggggga agtgctacgt
     1921 atcactatct caataagcat aatgtgttcc ggattatgtt accgcaaaga aattaacata
     1981 gggcttcagt acgccataaa tcaaccatga aaaaacgcag ccacgtcgtg agtattaacg
     2041 tggctacgat tgattatgcg tttacattca aacgtaacag ggattaatcc acataagctg
     2101 ccagcatttc ctgcgccgcc agtttaccca acgcattcgc tgcgaaagga ctgtcgccgg
     2161 tgagaagttt acggtcctta tgtactcgcc cggtgatgtc gtcattaatg attttcatgc
     2221 ccattttctt cagctcttcg ccgaagtacc aggtgagatg acctggcata tagccaatat
     2281 ctggcgtttg tttgtctgcg gcgtctggga atgcgcaaat ggaataacca ttcagtgggt
     2341 tatcgccgtg gcgaagcgcc agaaaagccg ccgggccgtg gcaaagggag ataacaaaac
     2401 ggtcattttt gattgcccac tgtaaagcgg cagctacgtc ctggctttca ggtaaaccaa
     2461 taagtgcgcc atgaccacca ggaacaaaga ttgctgcata ttcgctatca gcgttgaggc
     2521 tggcgacaac atccgcgagt ttcttcggat tgcggaacaa cgatttatgc tgctcaaaga
     2581 atggcattac tttttcatct ttgtgcggca tagcccagta ttcaaacttg gtcatcagac
     2641 cggaaatggt cgccacttcg aattcgaaac ctgcagcatg gagatgatac aacggcagca
     2701 acgtttcaat cggatggtta ccggtcgaga acagttttcc gttatcggtt ggcaaataac
     2761 gttcgtctgc ggcgatcacc agaattttat gtttaccgcg atacggtttt ggatagtcca
     2821 cgccatcaag atcagagaca ggactggtat attggctaag cgaatattct gaagggaaga
     2881 atgcattatc ttcagcaata tcgacctgcg gatttttact tgtttgaaca gtcatagtgt
     2941 attccttatt gttgcttagt tagggtagtc actatatttg cgactgagct ggtcgctatt
     3001 tttaaccagg ctggaaaatt gagtcaatat atcgaaagcg gtagtcggcg ggtgtaagta
     3061 tgtaatgatg atgctgcact ggtgttgaat attattcacg ccaaattttc cttattgagc
     3121 aacgtcgtcg caagtggcct caattatttt agaccattat cagaggtacg gggcgacggg
     3181 gagagattta gtgctgtgga atattgtggt gctgatgaat atttatatcc cggattggta
     3241 gagaaaaagc ttatttcatg cactattttt gcaaatattt ctatatggga ggcggttttg
     3301 ttgcagatta attcataaag aaatgcattt gggtcttggc tggaagagtc gctggtagat
     3361 gttattgtgc cagcaggggc agggtaaggt gctgtatcga tatgcattgg caattgcgtg
     3421 tgccgcagat ggagatgaaa tgccaataaa aaagcctgaa ttcgatgagg gacgaactca
     3481 ggctagtgtt tagttttaca gtaacacgcc gcagcacgtt ggctgattag aactggtaga
     3541 ctaagcccac accaacaacg tcatctgttg cgatgccatt gttgtcatag aaatcgtcat
     3601 ctttatccaa caagtttatt ttatagtcaa catatgttga gaagttctta ttgaaatagt
     3661 aggtcgctcc aatttcaaca tagttgacca gatcctgatc aacatactct tgatgaccat
     3721 tgttgttata gtcgttaccg ccgaggtctt tgccttttga ataaacatat gcaagagatg
     3781 ggcgcagacc aaaatcgaac tgatactgtg ctacagcttc aaagttctgg gttttatttg
     3841 cgatacccac attaccatat ggtgtcatat tgcgggtttc agcatacatc attgcaagat
     3901 aaacattgtt ggcgtcgtat tttgcaccaa atgtccatgc ttcagctgta tcaccaccag
     3961 caaatttgct gtattcattg tagtgatacc cgccaagagc tacttgagaa ttggtacggt
     4021 cagagtttga gtaagcggca cctaagctca aaccgaagtc aaagtcatat gaagtggaaa
     4081 gaccaaaacc atcaccgttc tggaagcgaa cgtcttcgtg accattttta gtaccttctt
     4141 gatcttctcc tgcattgctg ccttcgttgt taccttgata ctgcaatgca aagttcagac
     4201 cttccaccag accgaagaaa tcactattgc ggtaagtagc taccccattt gcacggcctg
     4261 ccatgaagtt atcagtccag gtataggagt cgccgccgaa tacaggaagc atatctgtcc
     4321 aggcttcaac gtcgtaaatc acaccatagt tacgaccata gtcaaaggag cccatctcgc
     4381 cgaacttcag acctgcaaaa gcaagtttga cataagtatt atcagaacct tcagtgtcgc
     4441 tagcctttgt tttggtcagt gcttgaccat aaccagttaa ctgatctgta atttgggtct
     4501 cacctttgaa gcctaactcg atgatagttt catcgccgtc atcgccaggg ttatcggaaa
     4561 aagtatgacg agcatcaact tttccgtaca gatccagttt attgccattt ttattataaa
     4621 tttcagccgc atttgctgcg ccagcaacta ataacgccgg gaccagcatt gccaga
//
LOCUS       DAZGVQ010000264         4526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000264 DAZGVQ010000000
VERSION     DAZGVQ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.267"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..338)
                     /locus_tag="ACHLAG_005052"
     CDS             complement(<1..338)
                     /locus_tag="ACHLAG_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0658123.1"
                     /translation="MHIKNCRQYTPENPDVPGAMYLKSEDGQDWYECQSLFSAETLKV
                     VYNSAGVITGISRVASVLWPVNQSVVEVADTEENRKADISGRWGFDGEKITDLLTAEK
                     ARGMKGDDINA"
     gene            complement(380..2107)
                     /locus_tag="ACHLAG_005053"
     CDS             complement(380..2107)
                     /locus_tag="ACHLAG_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP0658124.1"
                     /translation="MTVKISGVLKDGAGKPVPGCTIELKARRTTETVIVTTVAYGQPG
                     ETGSYSMDVEPGLYRVTLNTEGYAPSYVGDILVKADSAPGTLNKFLMDLEDAQYYPKA
                     LAELEAVAAEILKRAEASAASAEEAKKRAENARGPKGDKGDTGPQGIPGPKGDTGERG
                     PKGERGERGPQGLQGVKGERGEKGEKGEPGGPDATTAQKGIVQLSSATDSDDETKAAT
                     PKAVKAAMDKADGCLEKAKNGDDIPDKVQFLNTVGAARVYGRDIHTGAGEWTTGEFVA
                     WLKAKGAFDQPYWMMKASLHAGVNKVITDVGPGKLNLGGCVIEVMGKYEAAIVRVTIG
                     EYGATGFINGTVCTCTVYGDTQYFHWRVDYSTKNKPDTVSQRDASTTQKGVVQLSSDT
                     NSNDETKAATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPKGEKGDPGAQGPKGETGA
                     TGPAGPRGAQGPKGEKGDPGPRGERGETGPMGPPGASDGKSRVVGIRLGNKQTYAPQT
                     DSTTWAVDLDIGAMITGIGGYNDGNKTLIDRVSYRPLQVTFDGSQWRTVSVGEYVSSG
                     VSGFEYFPL"
     gene            complement(2257..2880)
                     /locus_tag="ACHLAG_005054"
     CDS             complement(2257..2880)
                     /locus_tag="ACHLAG_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP0658125.1"
                     /translation="MRYLYGAILFFTTLPAVMTFPAQAAGGHGAFSVGYAQVHPGGVP
                     VLSGTGARTGDLKGINVKYRYEFTDHLGGIASLSYASAKKSRTTMTGDKAFHYESLRG
                     RYVSLMAGPVWQVSEQLSLYGMAGMAHTRWSDSVQDYRRDEVTQGDVRVTTTASDGHS
                     ARHLTLAWGAGLQFNPADTVVVDLAYEVAGHGDWRTDAFIVGIGYRF"
     gene            complement(2949..>4526)
                     /locus_tag="ACHLAG_005055"
     CDS             complement(2949..>4526)
                     /locus_tag="ACHLAG_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003955761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP0658126.1"
                     /translation="FRINAPAAPASIELTPGYFQITVVPHPAVYDPTVQYEFWFSEKR
                     ITDTAQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGQASND
                     GEGYLEIFRGLIDEALLGKALKERIDASALRTEVTQLEEDIRQRLETDVAEVTRKIGE
                     AENSLTQLVAKKNEDQTLAIAQVSQQVDRVSSEITQTVSQSTEENARQIAQVRQYVDD
                     KGSEITSTTDKKLEDQSATIQQIQQVQSDTNDELAALYMLKVQKTKNGIPYVAGIGAG
                     IEDADGQTLSNILLQADRIAMITPENGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSS
                     GNPPTFSLTPEGRLTARNADISGHISANSGTLNNVTINENCVIRGKLSANQIEGDLVK
                     TVGKAFPRNNSYARGTITVTVYDDQGFDRQIIIPPVLFRGTKHQNVNSPNQQSYWYST
                     CKLQVLKNGAEIFHEPATDVSRVFSSVIDMPAGRGHVTLTFNVSSAGANNWTPTTYIS
                     DLLVVVMKKSTAGISIS"
BASE COUNT          882 a         1490 c         1009 g         1145 t
ORIGIN      
        1 gcgttaatgt catcgccctt catccctcgc gctttctccg cggtcagcag gtccgtgatt
       61 ttctccccgt caaaacccca gcgccccgag atgtcagctt tgcggttttc ttccgtatcc
      121 gccacctcca ccacactctg gttcacaggc cacagaactg acgccaccct gctaatgccg
      181 gtaatgaccc cggcactgtt ataaaccacc ttcagcgtct ctgctgaaaa taacgactga
      241 cactcatacc agtcctgacc atcctctgat ttcaggtaca tcgcacccgg cacatccgga
      301 ttttccggcg tgtattgtct gcaattcttt atgtgcatct gtaagtcctc ccggttacat
      361 gtctgaaaga tgatgcgctt tataatggaa aatactcaaa ccccgaaact cctgacgaca
      421 catactcacc cacgctgact gtacgccact gagaaccgtc aaatgtcacc tgaagcggcc
      481 tgtaagatac cctgtcaata agggttttgt ttccgtcgtt gtaaccgcca atccccgtaa
      541 tcatcgcacc aatatccaaa tccactgccc atgtagtgct gtctgtttgc ggcgcataag
      601 tctgtttatt acccagcctg atacccacaa cccggctctt cccgtctgat gccccgggcg
      661 gccccatcgg cccggtttct ccacgctcac ctctgggacc ggggtcgcct ttctcccctt
      721 tcggtccctg cgctccccgc ggaccagcgg gacctgttgc tcccgtttca cctttcggac
      781 cctgtgctcc ggggtcgcct ttctctcctt tcggaccggg aacaccacct cctgcagcag
      841 cctcttctgc ctttgttttc gcttcatttg ccacatccat tgccgccttc acggccttcg
      901 gcgtggctgc ctttgtttcg tcattactgt tagtatcact gcttaactgc accacgcctt
      961 tctgcgtcgt gctggcatcc cgctggctga ccgtatccgg tttgttcttt gtgctgtaat
     1021 ccacccgcca gtggaaatac tgtgtgtcgc cgtaaaccgt acaggtacag accgtgccat
     1081 taataaaccc cgttgcaccg tattcgccaa tggtgacccg gactatggct gcctcatact
     1141 tccccatcac ctcaatgaca caaccaccca gattaagttt tcccggtccg acatccgtga
     1201 ttaccttatt gactcccgca tgcagtgatg ccttcatcat ccagtaaggc tggtcaaatg
     1261 cccctttggc tttcagccag gcaacaaact cgcccgtggt ccattcaccg gcccccgtat
     1321 gaatgtcccg cccgtatact ctggctgctc ccacagtgtt cagaaactgc accttatccg
     1381 gaatatcgtc accgtttttc gctttctcaa ggcatccgtc cgctttgtcc attgccgctt
     1441 tcaccgcttt cggggtggct gccttcgttt catcatcact gtccgttgcg ctgcttaact
     1501 gcacaatgcc cttctgtgcc gtcgtcgcat ccggtcctcc cggctcgcct ttttcgccct
     1561 tctctccccg ttcacctttc acaccctgaa gcccctgcgg ccccctctca ccacgctcac
     1621 cctttggccc acgctcgccg gtatcgcctt ttggcccggg aataccctgt ggcccggtgt
     1681 ctcccttatc ccccttcggt ccccgcgcat tctctgcccg tttcttcgct tcctctgcac
     1741 tcgccgctga cgcttctgca cgtttcagga tttccgcggc caccgcttcc agctctgcaa
     1801 gggctttcgg gtaatactgt gcgtcctcca ggtccatcag aaatttattc agcgttcccg
     1861 gtgcagagtc cgccttcacc agaatgtcac ccacatatga cggcgcgtac ccttccgtgt
     1921 tcagcgtcac ccggtacaac cccggctcaa catccatact gtaactgccg gtttcccccg
     1981 gctgaccata cgccaccgtg gtgacaatca ccgtctccgt tgtgcgtcgc gctttcagct
     2041 ctatcgtgca tcccggtacc ggcttaccgg ccccgtcttt caatacacca gaaattttaa
     2101 ccgtcatact tttccaccaa taaaaaagcc cgcagcagta atgccacggg cttcaggaca
     2161 gtgtaacttt acgtttcctc agacgcagtt caccccataa ggcggatgaa catgcgtaca
     2221 ttgcaatatt tgcagaaaat aaagcagcat ctgccgtcag aaacggtatc cgatcccaac
     2281 aataaacgca tccgttcgcc agtcaccatg accggccact tcgtacgcaa ggtcaaccac
     2341 caccgtatcc gccggattaa actgaagccc tgccccccat gccagcgtca gatgacgcgc
     2401 agaatgacca tcactggcag tggtggtcac cctgacatcc ccctgtgtca cttcatcacg
     2461 ccggtaatcc tgaacactgt cagaccagcg ggtgtgcgcc atcccggcca tgccataaag
     2521 gctgagctgc tcactgacct gccagaccgg ccccgccatc aggctcacat aacggccacg
     2581 cagactttca taatgaaatg ctttatcccc ggtcatcgtg gttctgctct ttttcgcaga
     2641 ggcgtaactc agcgaggcta tgccacccag atggtccgtg aattcataac ggtatttcac
     2701 gttgatccct tttaaatcac ctgtacgggc accggtaccg gacaataccg gcacgccgcc
     2761 cgggtgaacc tgagcatatc ccacggaaaa tgcaccgtgt ccgcctgcag cctgtgcagg
     2821 aaaagtcatt actgccggca gggtggtaaa aaataaaatg gctccgtata aataccgcat
     2881 gattacctct ttgttttcag tcaataaaaa aggcaccact tcgggtgccc gtccgggtta
     2941 ataaaccgtc agctgatact gattcccgcc gtggattttt tcatcaccac aaccagtaaa
     3001 tcactgatgt acgtcgtcgg tgtccagttg ttcgcgccgg cagacgacac attaaacgtc
     3061 agggtgacat gaccccgtcc tgctggcatg tcaatcaccg atgagaacac ccggctgaca
     3121 tccgttgccg gttcatgaaa aatctctgcc ccgtttttca gcacctgcag cttacaggtt
     3181 gaataccagt acgactgctg attcgggctg ttgacgttct ggtgtttcgt cccgcgaaac
     3241 agtaccggtg gaatgataat ctgccggtcg aagccctggt catcgtaaac cgtgacggtt
     3301 atcgtgccac gggcataact gttattccgg ggaaaggctt tccccaccgt cttcaccaga
     3361 tcgccttcaa tctggttcgc agacagtttc cctctgatga cacagttctc gttaatggtg
     3421 acattattga gcgtgcccga gttcgcactg atatgtccgc tgatgtccgc attgcgggct
     3481 gtcagcctgc cctccggcgt cagggaaaac gtcgggggat tgccggatga cgtgatactc
     3541 accgcaaaca gtcgcttcag gaacacgtcg ttcataaaca gctgattccc ctgcgccaca
     3601 aacagcggcg tggtgttgcc gttctccggg gtaatcatcg cgatacggtc cgcctgcagc
     3661 agaatattgc tcagcgtctg gccatcagca tcctcaatcc ccgcaccaat accggcaaca
     3721 tacggaatgc cgtttttcgt tttctgcacc ttcagcatgt acagcgctgc cagctcatca
     3781 ttcgtgtctg actggacctg ctgtatctgc tgtatggtgg cgctctggtc ttccagcttt
     3841 ttatccgtgg tcgaggtgat ttcactccct ttgtcatcca cgtactggcg gacctgcgct
     3901 atctgcctgg cgttttcctc cgtgctctgg ctgactgtct gtgtgatttc actgctcacc
     3961 cggtccacct gctgactcac ctgcgcgatg gccagcgtct ggtcctcatt ctttttcgca
     4021 accagctgcg tgaggctgtt ttccgcctcc ccgatttttc gggtcacttc cgcaacatcc
     4081 gtctccagtc gctggcggat gtcttcttcc agttgcgtga cctcagtacg cagggctgac
     4141 gcatcaatac gctctttcag tgccttcccc agcagcgcct catctatcag cccacggaaa
     4201 atttccagat acccttcacc atcattgctg gcctgcccgc tggcctccac aaacgcagat
     4261 ttccccacca ggttgacgct gcgcacataa aaccagaaat ccttccccgg cttaatgtgc
     4321 gggccggaga cgctccactg gctgccggta cccagataac gggcagaggt ttccacctgt
     4381 gccgtgtccg tgatgcgttt ttctgagaac cagaattcat actgtaccgt cgggtcatac
     4441 accgcaggat gcgggaccac cgttatctga aaatagcccg gcgtcagttc aatgctggcc
     4501 ggtgccgccg gtgcattaat ccggaa
//
LOCUS       DAZGVQ010000265         4504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000265 DAZGVQ010000000
VERSION     DAZGVQ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.268"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            215..661
                     /locus_tag="ACHLAG_005056"
     CDS             215..661
                     /locus_tag="ACHLAG_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFP0658127.1"
                     /translation="MAALTASCIDLNIQGNGAYSILKQLATMALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGIAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
     gene            672..2123
                     /locus_tag="ACHLAG_005057"
     CDS             672..2123
                     /locus_tag="ACHLAG_005057"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFP0658128.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSHLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYVLAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2113..3183
                     /locus_tag="ACHLAG_005058"
     CDS             2113..3183
                     /locus_tag="ACHLAG_005058"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP0658129.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAAFKNCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            3183..>4504
                     /locus_tag="ACHLAG_005059"
     CDS             3183..>4504
                     /locus_tag="ACHLAG_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFP0658130.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQP"
BASE COUNT         1040 a         1149 c         1186 g         1129 t
ORIGIN      
        1 tatttccccg atgcattgtg agtaaatcac aaaaataatg aataactcat taatgattca
       61 tgtggtttat ttaaataacc attatgtgca ttactccgca aatctgacct ttcactctgt
      121 ctcaaattgt cattgtttac cgacatatcg cctctcatca cttaatcgct ccaccattac
      181 aattgattaa aatatatttc atggagtccg attaatggca gctcttactg caagctgtat
      241 tgacctgaat attcagggca atggcgctta ttccattctg aagcagttgg cgacaatggc
      301 gttacaaaac ggttttatca ccgactcaca ccagttcctg caaaccctgc tgctgcgcga
      361 aaaaatgcac tctactggat ttggttccgg catcgccgtg ccgcacggta aaagcgcctg
      421 cgttaaacaa ccgttcgtgt tattcgcccg caaagcgcag gctattgact ggaaagccag
      481 cgatggcgaa gacgtcaatt gctggatctg cctcggcgtg ccgcaaagcg gcgaagagga
      541 tcaggtcaaa atcatcggca cactgtgtcg caaaattatt cacaaggaat ttattcatca
      601 actgcaacag ggcgataccg accaggtgct tgccttgtta aatcaaaccc tcagctcata
      661 aggaagtggc gatggagtca tccttacgta ttgtcgcgat caccaactgc cccgccggga
      721 tcgctcacac ctacatggtg gcggaagccc tggaacagaa agcccgttct ctcggtcata
      781 ccattaaagt tgaaactcaa ggatcaagcg gcgtcgaaaa ccgcttatcc agcgaagaga
      841 ttgccgctgc cgattacgtc attctcgcta ccgggcgtgg cctgagcggt gacgatcgcg
      901 cgcggtttgc cgggaagaag gtttatgaga ttgccatctc ccaggcgctg aaaaatatcg
      961 atcagatttt cagtgaatta ccaacaaact cgcatctttt tgctgcagat agcggcgtga
     1021 agcttggcaa acaggaagtg caaagcggca gcgtgatgag ccacctgatg gcgggcgtct
     1081 ctgcggcgct gccgtttgtc atcggcggtg gtatcctggt ggcgcttgcc aacatgctgg
     1141 tgcagttcgg tttgccgtat accgatatgt cgaaaggcgc gccgtcgttt acctgggtag
     1201 ttgaatccat cggttacctt ggcttcacct ttatgatccc catcatgggc gcttatattg
     1261 cctcgtcgat tgccgataaa ccggctttcg ctccggcgtt tctggtttgc tatctggcga
     1321 acgacaaagc gctgctcggc acccagtcgg gtgcgggatt cctcggcgcg gtggtgctgg
     1381 ggctggcgat tggctatttc gtcttctggt tccgcaaagt gcgccttggc aaagcattac
     1441 aaccgctgct cggttccatg ctgatcccgt tcgttactct gctggttttc ggcgtgctca
     1501 cttactacgt tatcgggccg gtgatgtccg acctcatggg cgggctgctc cacttcctga
     1561 ataccattcc tccatcaatg aagtttgcgg cagcgtttct ggtcggtgcg atgctggcgt
     1621 tcgatatggg cggccccatc aataaaaccg cctggttctt ctgcttctca ctgctggaaa
     1681 aacacattta tgactggtac gccatcgtcg gcgttgtcgc gttgatgccg cccgttgcgg
     1741 cgggtcttgc gacgttcatt gcgcccaaac tgtttacccg acaggaaaaa gaggctgcca
     1801 gtagtgccat tgtggtcggt gccacagtcg caaccgaacc ggctatacct tacgttctgg
     1861 cagctccgtt gccgatgatt accgccaata cgctggcggg tggcattacc ggcgtgctgg
     1921 tgatcgcttt cggaatcaaa cgtctggcac ctggtttggg tatctttgac ccgctgatcg
     1981 gcctgatgtc gccggtgggt tcgttttatc tggtgctggc gattggcctc gcgctgaaca
     2041 tttcattcat tatcgtcctg aaaggattgt ggttacgccg caaagcgaaa gccgcgcaac
     2101 aggagcttgt ccatgaacat tgagttactg caacagttgt gcgaagccag cgccgtcagc
     2161 ggcgatgaac aggaagttcg cgacattctg ataaacacgc tggaaccttg cgttaatgag
     2221 atcacctttg atggtctggg cagctttgtt gcccgtaagg ggaataaagg tccaaaagtt
     2281 gccgttgtcg ggcatatgga tgaagtcggc tttatggtca cccacatcga cgagagcggt
     2341 tttctgcgtt tcaccaccat tggtggctgg tggaatcagt caatgctcaa ccaccgggta
     2401 accatacgca cacacaaggg atttaaaatc cctggtgtga ttggttccgt cgcacctcat
     2461 gcgttaacgg aaaagcaaaa gcaacagccg ctgtcatttg atgagatgtt cattgatatt
     2521 ggcgcgaaca gtcgcgatga ggtggaaaaa cgcggtgtgg cgatgggcga ttttattagc
     2581 ccggaagcca attttgcctg ctggggcgaa gataaagtgg tcggcaaggc gctggataac
     2641 cgcatcggct gcgcaatgat ggctgaacta ttgcagacgg tgaataatcc cgaaattacg
     2701 ctgtatggcg tcggcagcgt ggaagaagaa gttgggctac gcggggcaca aacctcggcg
     2761 gaacacatta aaccggatgt ggtgattgtg ctggataccg ctgtcgcagg tgatgttccg
     2821 ggcattgata acattaaata cccgctgaaa ctgggccagg ggccggggct gatgctgttt
     2881 gacaagcgct acttccccaa ccagaaactg gtagcagcgt ttaaaaactg tgccacgcag
     2941 aatgatttac cgctgcaatt ttccaccatg aaaaccggag cgacggatgg cgggcgctac
     3001 aacgtgatgg gcggcgggcg tccggttgtc gcgctgtgtc tgccaactcg ttatctgcac
     3061 gccaacagcg gtatgatttc aaaagccgat tacgatgctc tgctcacgct gatacggggt
     3121 tttctgacca ccttaactgc ggagaaagtc aacgcgttta gccagttccg tcaggtggat
     3181 taatgctaaa cgaacgccag ttaaagattg tcgatctgct ggagcaacag ccgcgcacgc
     3241 ctggtgagct ggcgcaacag actggcgttt caggcaggac catcctgcgt gatattgact
     3301 atctcaactt tacccttaac ggcaaagccc gcatttccgc cagtggtagt gcgggctatc
     3361 agctggaaat cttcgagcgc cgcagctttt ttcagttgct gcaaaagcac gataacgacg
     3421 atcggctgct ggcactgtta ttactgaata cctttactcc ccgtgcgcaa ctcgcctcgg
     3481 cgcttaattt gccagaaacg tgggtcgcag agcgtctgcc ccggttaaaa cagcgttatg
     3541 aacgcgcttg ttgcctggcc agccgccccg gtttgggcca tttcattgat gagacagaag
     3601 agaagcgcgt tatcttgctg gcgaacttgc tgcgcaaaga tccgttttta attccgctgg
     3661 cgggcgtaac acgagacaac cttcagcatt tatccacggc ctgcgacaac caacaccgct
     3721 ggccgctcat gcagggtgat tatctctcca gcctgattct ggcgatttac gccctgcgta
     3781 atcaactgac cgatgagtgg ccgcaatatc ccggtgacga gataaaacaa atcgttgaac
     3841 atagcggtct gtttcttggt gataacgctg taagaaccct gacaggtttg atagagaaac
     3901 agcatcagca agcgcagata atttctgccg ataatgtgca gcggttgctg caaagggtgc
     3961 cgggcatcgc gtcattgaat attattgata cgcagctggt tgagaacatt accgggcatt
     4021 tattacgttg ccttgccgca ccggtgtgga ttgctgagca ccgccaaagc agcatgaata
     4081 acctgaaagc cgcctggcct gcggcctttg atatgagtct gcactttatt acgctactgc
     4141 gtgaacagct cgatattccc cttttcgaca gcgatctgat cggtttgtat tttgcctgtg
     4201 cgctggaacg gcatcaaaac gaacgccagc cgatcatttt gctctcggac cagaacgcga
     4261 ttgccactat taatcagctc gccattgagc gcgatgtttt acattgtcgg gtgattattg
     4321 cccgtagctt aagcgaactt gttgccattc gcgaagagat tgagccgtta ttgatcatta
     4381 acaacagcca ttatttactg gatgacgcgg taaataatta catcaccgta aaaaatatca
     4441 ttacggctgc cggtatcgaa caaataaaac attttctggc gacggcattt attcgccagc
     4501 aacc
//
LOCUS       DAZGVQ010000266         4454 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000266 DAZGVQ010000000
VERSION     DAZGVQ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.269"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(171..1868)
                     /locus_tag="ACHLAG_005060"
     CDS             complement(171..1868)
                     /locus_tag="ACHLAG_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HFP0658131.1"
                     /translation="MEHQLNKKLLNSSISLILASGLMMSTANAADDVKLKATKTNVAF
                     ADFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDDSSFDPKTMDNRNVVDTYKIGIDKAI
                     EAAKKSTPTLATLMDEGVKFTNGYVAHGVSGPSRAAILTARAPARFGVYSNSDAQDGI
                     PLEETFLSELLQNHGYYTAAIGKWHLSKISNVGVPEEKQTRDYHDNFTTFSAEEWQPQ
                     NRGFNYFMGYHASGVSLYNSPSLFRNRENIKAEGYISDQLTDEAIGVIDRASKLEQPF
                     MIYLAYSAPHLPNDQPAPKNYQEKFNTGSKTADNYYASIYSVDQGVKKILNTLKEKGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQAGYKGQPYPGGMHTPMFMWWNGKLKTGDYDKL
                     VSSMDFYPTALDAADIKIPASLKLDGVSLLPYVQKGADKKSAAGEPHKYLSWITSYSH
                     WWDEENTAFWNGYHKFVRKESDDYPHNPHTEDLSQFAYTVRSNDYSLVYTYAENKLAL
                     YDINDLQQTKDLSASHPDVVKNMKAVMIDFITSSQKPISDINQAKFENIKKSLTNVK"
     gene            complement(1964..3100)
                     /locus_tag="ACHLAG_005061"
     CDS             complement(1964..3100)
                     /locus_tag="ACHLAG_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFP0658132.1"
                     /translation="MRKISMVVCSVVSALMLCEATQAAEIYNKDGNKLDFYGRVKARH
                     YISDGSMDGDRTFARLGFKGETQINESLTGFGQWEYQFLGFKTESEVRNDKNRLAFAG
                     IRHNILGSLDYGRNYGVAYDVGALTDVLPDFGGDSWTYSDNYMTGRTTGVLTWRNTDF
                     FGLVDGLTFSAQYQGKNDRDNLLEANGDGYGFSVSYDYEGFGIVAAYTNADRTSLQEK
                     EGRKRFMTSKDSEGKEVITPRWIAGGKHAEFAGVGVKYNDNDLYLAADYSETRNMTPF
                     GSAGIADKARNIEVVAQYMFDFGLRPSLAYVQSKGMDVKGGIVNYGDQSLVEYIDVGA
                     NYFLNKNMSLLIDYKINLIDESEFTKKAGVATDNILAVGMMYQF"
     gene            3776..3984
                     /locus_tag="ACHLAG_005062"
                     /pseudo
     CDS             3776..3984
                     /locus_tag="ACHLAG_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            3971..4160
                     /gene="tnpB"
                     /locus_tag="ACHLAG_005063"
                     /pseudo
     CDS             3971..4160
                     /gene="tnpB"
                     /locus_tag="ACHLAG_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase.; frameshifted; incomplete; partial
                     in the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(4163..4351)
                     /locus_tag="ACHLAG_005064"
     CDS             complement(4163..4351)
                     /locus_tag="ACHLAG_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFP0658133.1"
                     /translation="MGKRYKITATIIKPGNMPVQWLRYQDSEMTFAECEKLFAPAKEP
                     GRYIRVRVEDFRCEVVKA"
BASE COUNT         1225 a          983 c          882 g         1364 t
ORIGIN      
        1 agaagctttc tttgtttcaa gaacagattt gattaactcc ttacgttgtg tacaccaaat
       61 ctcgtcagat gaacagtatt ttttattttc gtgtttgttc attatttatt gctctaactg
      121 atttatttcc ctatatccag ggagtagata tatctactcc ctgcttatta tcatttcacg
      181 ttagttaaag atttttttat attctcaaat ttggcttggt tgatatcact gatcggtttt
      241 tgacttgacg tgatgaagtc aatcataaca gccttcatat tcttaactac atcaggatgg
      301 gatgcagaga gatctttagt ttgttgcaga tcgttgatat cgtacagagc tagtttattc
      361 tctgcatagg tgtaaaccag agaataatcg ttgcttctta cagtgtatgc aaactggctt
      421 aagtcttctg tatgtgggtt gtgcggataa tcatcagact cttttctcac gaatttatga
      481 tatccgttcc agaatgcagt gttctcttcg tcccaccagt gagaataaga ggtaatccag
      541 gacagatatt tgtgaggttc gccagctgca gatttcttat cagctccttt ctgtacatat
      601 ggcaataatg atacaccatc tagtttcaga gaagcaggga tcttgatatc tgcggcatct
      661 aaagcggtcg gatagaagtc cattgatgaa accagtttgt cataatcacc cgttttcagc
      721 ttgccattcc accacatgaa cataggtgta tgcataccac ccggatacgg ttggcctttg
      781 taaccagcct gcgcaccatt cagtggcagt ggaccatcaa ttacagcacc gttatcagag
      841 gtgaacagaa tgatggtgtt gtcgtactga cctttttcct taagtgtatt caggatcttt
      901 ttaaccccct ggtcaacgga gtaaattgac gcataatagt tatcagcagt cttgctacca
      961 gtgttgaatt tctcctgata gtttttaggc gcaggctgat cgttaggcag gtgtggtgca
     1021 ctgtatgcca gataaatcat aaatggctgt tccagtttgc ttgcacggtc aattacacca
     1081 atagcttcat cagttaattg atcactaatg tacccttcag ctttaatgtt ttcacggtta
     1141 cggaacaatg acggggagtt ataaagtgaa acgccggatg cgtgatagcc catgaagtag
     1201 ttgaagccac gattctgagg ttgccattct tctgcggaga aggtggtgaa gttatcgtgg
     1261 tagtcacggg tttgtttttc ttcaggcaca cctacgttgc tgatttttga taagtgccac
     1321 ttaccgattg ctgcagtgta ataaccatga ttctgaagta attcagacag gaatgtttct
     1381 tccagaggga taccatcctg tgcatcagaa tttgaatata caccgaagcg agcaggagct
     1441 cgcgccgtca ggattgcggc acgtgaaggg ccagagacac cgtgtgcaac atagccgtta
     1501 gtgaatttta caccttcgtc catcagggtc gctaatgttg gagtggattt ttttgcagct
     1561 tcaattgctt tatcaatacc tattttgtat gtatcaacaa catttcggtt atccatcgtt
     1621 ttagggtcaa atgaactatc atcaaaaggc aattgtccat accctaaatc atccattgtt
     1681 aaaacaatga tatttggctt acctttagtg ctgtattccg tcggcgtgaa gtccgcaaag
     1741 gcaacattgg tttttgttgc tttgagctta acatcatctg ctgcatttgc agtactcatc
     1801 atcagaccag atgctaatat aagtgatatt gatgagttaa gcagtttttt atttaactga
     1861 tgttccatat ggtattcctt ctgagttgca tcacgaacat ttattaataa taaaagcctc
     1921 tgatataaat aaaaactatg taaatcgttt aaatggatac ctgttaaaac tgatacatca
     1981 taccaacagc aagtatatta tcggttgcaa ctccagcctt tttggtaaat tcgctttcat
     2041 caataaggtt aattttgtaa tcaataagaa gtgacatgtt tttgttcaga aagtagtttg
     2101 cgccaacatc aatatactca acaagagact gatcgccata gtttactatt ccacctttaa
     2161 catccatccc tttggactga acgtaagcca gtgaaggacg caggccgaaa tcaaacatat
     2221 actgagccac aacttcaata ttccgagctt tatctgcgat gcctgcgcta ccaaatggag
     2281 tcatatttcg ggtttcagag tagtcagctg ccagatacag gtcattatca ttatacttga
     2341 ccccaacacc tgcaaactcc gcatgttttc ccccggcaat ccagcgaggc gtaataactt
     2401 cttttccttc tgaatctttc gatgtcatga aacgtttacg gccttctttt tcctgcagac
     2461 tggttctgtc ggcattagtg taagcggcaa cgattccaaa accttcatag tcatagctta
     2521 cggagaaacc atatccatca ccattagctt caagtaaatt atcacggtca tttttaccct
     2581 gatattgagc agaaaaagta agtccatcaa caagcccgaa gaaatctgta ttgcgccagg
     2641 tcagaactcc ggttgttctg ccggtcatat agttatcaga gtaagtccag ctgtcaccac
     2701 caaagtccgg aagtacgtca gtcagagccc ccacatcata ggcaacacca tagtttcgcc
     2761 cataatcaag tgaaccgagt atattgtggc gaatacctgc aaatgccaga cgatttttat
     2821 cgttgcgtac ttcactttct gtcttaaacc ccaggaactg gtattcccac tgaccaaagc
     2881 cagtcaggct ctcattaatt tgtgtttcgc ctttgaaacc aagacgtgca aatgttctgt
     2941 cgccatccat agaaccatca gagatgtaat gacgggcttt aacgcgacca taaaaatcca
     3001 gtttattacc gtctttatta tagatttctg ctgcctgtgt tgcctcacac agcattaatg
     3061 cagaaacgac agagcacaca accatactta tttttcgcat aaaaaaatcc cctgtgcaca
     3121 tttttagtat ggttttaaaa attaaactat ggcgaaaaaa ttatcactat gcaaaaatgt
     3181 aacaaaatat tgctaggtga tctatattgt tgtttttatt tgaggttgta tattttctag
     3241 tgtatttgac ttttctttct caagtgttat ttcttgacct ttatcacaga accatctccc
     3301 tgggtatccc catcacattg catgaaaagc aagttttacc aatcttgatt ttcagtgtta
     3361 aataagatta ataactgtaa ttctgcaaaa aaaagaacac catatgtagt atgactacgt
     3421 attccactct gttcgttcac ggactggcat cgaagtctga ggtgtactgg caatagcgga
     3481 tacagtcatt tgttcttttt ttaagcattc atccgatgat acttttcctg aatgctaccg
     3541 gagtggtatt cccctgccgt gagggagggc gctgacggtt ataaaaatcc tgatatatac
     3601 ccattcccgt atgacagcga tggcgtcaca tctctgttct accatcttca cagaaatatt
     3661 tcgttcatta gcattccggt attcgtataa ccaccttcgg tggagaaaga ataaacaatc
     3721 cctggaatgc gatgagcgct tgaccagaaa agcatcatat tgcttcatgg cgcatcagcg
     3781 accacaggat gccgcaaaag ccgaaaaacg gaccagagcg cctgaaatca ttcctcgcct
     3841 cagtatctgt tgcccatgga gtggccgata acagatcatt gtcggatgag ccttatcccc
     3901 ttccttcccg gtggtattcg gatgtgctgc cagccttctc agttaaccga tgttttccgg
     3961 gctttttgcc atactgagga ctgataacgt attcctggtc gtaaaacccg tcaatatgcg
     4021 ccggggcatc gacaaaactg acacagtacg tccggaaaga acttaacgct gcatggcacg
     4081 acaatgctgc ctttgtcttt accaacaagg cccgttcccg cataaaagtt ctacgccggg
     4141 ataaacatgg cgtatggctg cgtcaggcct tcactacttc acaccggaag tcttccaccc
     4201 tgacacgaat ataccgtccg ggctctttcg caggtgcaaa cagtttttca cattcggcga
     4261 atgtcatttc tgaatcctgg taacgcaacc actgaaccgg catattaccg ggtttgatta
     4321 ttgtcgcagt tattttatat cgtttcccca tatcgcctct catcctgaca acaaaattga
     4381 ggtagcctga gtttaacgga cactccttcc tgaaatagaa tggcatcaga aggagctaat
     4441 aatgagcaga aaaa
//
LOCUS       DAZGVQ010000267         4390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000267 DAZGVQ010000000
VERSION     DAZGVQ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.270"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..209)
                     /locus_tag="ACHLAG_005065"
     CDS             complement(<1..209)
                     /locus_tag="ACHLAG_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP0658134.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHLAG_005066"
     tRNA            complement(324..399)
                     /locus_tag="ACHLAG_005066"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHLAG_005067"
     tRNA            complement(406..480)
                     /locus_tag="ACHLAG_005067"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHLAG_005068"
     tRNA            complement(597..681)
                     /locus_tag="ACHLAG_005068"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHLAG_005069"
     tRNA            complement(690..765)
                     /locus_tag="ACHLAG_005069"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHLAG_005070"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHLAG_005070"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP0658135.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHLAG_005071"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHLAG_005071"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFP0658136.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEEILSQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGSGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHLAG_005072"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHLAG_005072"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFP0658137.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTHLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1125 a         1099 c          983 g         1183 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ctaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa acggttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt tcaggggagc taatgctccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagcaataa agcgccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgaaagat aaggtgccaa tccttcttgt tcgaagagtt ccagcgccgc gcgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacgacgca ttgccatgtt gatcccggat
     2461 ccaatgacta tttgcgccgc atcgccggtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttaa cacgaacttt atctgcaccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatagccgcc
     2641 gccgggcctt gttccaaacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gaccccggcg accacggcca gcctgctggt attctgcaac gcaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aatcactggc
     2821 agcacggcta cactaccgcc atccagctga ctcaatatct cttcagcatt aagtaactgg
     2881 ataggttcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gatgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggccat
     4201 ccgtcaggat ggccttctgc ttaatttgat gcctggcagt ttatggcggg cgtcctgccc
     4261 gccaccctcc gggccgttgc ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac
     4321 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     4381 cctttcgttt
//
LOCUS       DAZGVQ010000268         4343 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000268 DAZGVQ010000000
VERSION     DAZGVQ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.271"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..128)
                     /locus_tag="ACHLAG_005073"
     CDS             complement(<1..128)
                     /locus_tag="ACHLAG_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFP0658138.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGDRYQPCVNISAEP"
     gene            complement(139..837)
                     /locus_tag="ACHLAG_005074"
     CDS             complement(139..837)
                     /locus_tag="ACHLAG_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0658139.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGREYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQLSELTAMKASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRGMV
                     ANFGGFLSINKLSQ"
     gene            complement(837..1178)
                     /locus_tag="ACHLAG_005075"
     CDS             complement(837..1178)
                     /locus_tag="ACHLAG_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0658140.1"
                     /translation="MDEIKTLHWCPREGMQVTEKPSVVTVKFGDGYQQRRPAGLNAQL
                     KTFQVVFRVTTDAEREALSAFLSWHGGYRAFLWKPPKHNRTVRVVCREWSITDNARYS
                     DFSCTIEQVVN"
     gene            complement(1171..>4343)
                     /locus_tag="ACHLAG_005076"
     CDS             complement(1171..>4343)
                     /locus_tag="ACHLAG_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0658141.1"
                     /translation="ASRMGSEYYRTLQNGGRQAAAAAREQRRALAELNSQLTEIRASA
                     VGMTGAFAGAFATGHLISLADEWSSVNARLKQASQSSDEFASSQKVLMDISQRTGTAF
                     SDNAALFARSAASMREYGYSADDVLKVTEAISTGLKISGASAAEAGSVITQFSQALAQ
                     GVLRGEEFNSVNESGDRIIRALAAGMGVARKDLKAMADDGQLTADKVVPALISQLGIL
                     RDEYAAMPETVSGSITKVENAFMAWVGGANEASGATKTLSGALNGVAGNIDTVATAAG
                     VLVAVGVARYFGNLASGAMSATAGLVTAARNEVALAEAQLRGTQIATARARAAVYRAQ
                     QAVAAARGTEMQIAAEARLAVTQERLNRNIAARTAAQNALNSTTAVGSRLMSGALGLV
                     GGIPGLVMLGAAAWYTLYQNQEQAMESARQYALTIDEIAHKTPSMSLPEASDNEGRTR
                     EALTEQNRLIDEQASRVKSLQEKIAGYQYVLANPGWTTDNGFMINHLTSVKTVTEGLA
                     QATEQLAVEQSRLAQMQEKAQSIQDVLAGLEERRVVLIRQQAAEQNKAYQSLLVMNGQ
                     HTEFNRLLGLGNELLQQRQGLASVPLRLPQATLDDKQQSSLNNTERQLALSRLKGEEK
                     ERARLGYAADDLGLVGDTYQEARQRYIRNSMEAWRNNEANKPKSRGGKSETEKAEDSF
                     SRLLKQQKEQLALAGKNTELAKLKYQTSQGELKILTEIQKQELLRNAALIDQKKIREQ
                     LRAREETLKNDNADARASNDAELLGYGQGERIRERMRELKQIRDGYRQKDADLQSQYQ
                     TGDISEDFYRQALAQNAQYLSERLKDQEAFYAESDAQRADWQKGLQEGLSNWVDSASD
                     YASQAAQLATDGISGMVNNITEMLNGNKVEWRSWAALILQEISKVLMNAAIVNGIKMA
                     ANSMSGAGGFFGSIGNWLGGAVANAKGGVYTSANLSAYSNSIVDTPTYFAFAKGAGLM
                     GEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYNIAIQNDGANGQIGPEAAG
                     TLVQLIDQRVQAVMLSMRRDGGMLSG"
BASE COUNT          850 a         1469 c         1020 g         1004 t
ORIGIN      
        1 ggctctgcag agatattcac acacggctgg taccggtcac cctccggcgt gcttatcacg
       61 aagccgcaag actccgcagg cgcacaccgc cgggcatgcg ccagaatcgc tgattcagtc
      121 tgtgtcataa aatgggattt actgcgaaag tttattaatg gaaaggaaac cgccgaaatt
      181 agccaccatg ccgcgcagct cacacccgcg catgcacttg ctgcatctgt ccttacggat
      241 atccgtggtg ggtttatcga actcatccgc caccgcccca ccggtgtaac cacactcatc
      301 agagcggtag gtccacatac aggtgttcgc cagcatgatg cgaccgggaa acagcgcccc
      361 gtccgtctcg gtcggtgtcg ccagcacaaa cgaggccttc atggccgtca gctctgataa
      421 ctgctccacc acccagcggt cgctcagctc ctgctccggg tccgcttccg gattgcccgc
      481 cacaaaattc accgcatcca gaaaacgcgc atacacccgg cggcggacca ccgtggcacc
      541 caccagactc tgcaggtcct ccgccatccc ggtgacaaga ccaaacagat tggacaccgt
      601 cagcgacggg cgggcactgc tgccctttcc gttcatctca aagccgctgc cgtcaatcgg
      661 gtatgcctga tattcccgcc cctgccaggt caccggctcc cctttttcat tcagctcatt
      721 gcagaaaaaa tagcgctcac cgccctgcac cgtcaggtca atttcccaga gcaccacccg
      781 cggtgactgc tctgatttaa ccgactcgtt cagactttct tcatgaatat cctgcatcag
      841 ttcaccacct gctctatcgt gcaactgaaa tcactgtacc gggcgttatc cgtaatactc
      901 cactcccggc acaccaccct gaccgtccgg ttatgtttcg ggggcttcca caaaaaagcc
      961 cggtaaccac catgccatga cagaaacgcg gacagtgcct cccgctcagc atccgttgtc
     1021 acccgaaaaa ccacctgaaa ggtcttcagt tgcgcattca gtcctgccgg acgacgctgc
     1081 tgataaccgt cgccaaactt caccgtcacc accgacggtt tctccgtcac ctgcatccct
     1141 tcccggggac accagtgaag ggtcttaatc tcatccactc agcattcctc cgtcacgacg
     1201 catggataac atcaccgcct gtacccgctg gtcaatcagt tgcacaaggg tgcccgccgc
     1261 ttccggccct atctgcccgt tagcgccgtc attctgaatg gcgatgttgt acaccgggga
     1321 atacaccaga cccgcactgc cgttcatact gcccaccgca cgcacgccga gtgaaccatc
     1381 cgctgcccgg gtcagaggca ttatggcttc aggtccggcc tcccccatca gccctgcccc
     1441 ttttgcaaag gcaaagtacg tgggcgtgtc cacaatactg ttactgtatg cactcaggtt
     1501 tgccgaggta taaacgccgc cttttgcatt tgccaccgcg ccacccagcc agttgcctat
     1561 actgccgaaa aatcctcctg caccggacat actgtttgcc gccatcttaa tgccgttgac
     1621 aatggccgca ttcataagaa cttttgatat ttcctgcagg attaatgcag cccagctgcg
     1681 ccattccact ttatttccgt tcagcatctc cgtgatgtta ttcaccatcc ctgagatacc
     1741 gtctgtcgca agctgtgctg cctgtgaagc gtaatctgat gcgctgtcca cccagttact
     1801 tagcccttcc tgcagccctt tctgccagtc agcacgctgc gcatccgatt cggcataaaa
     1861 agcctcctgg tctttaaggc gctcactcag atactgcgca ttctgcgcca gcgcctgtct
     1921 gtaaaaatcc tcactgatat cccctgtctg atactgagac tgcaggtccg catccttctg
     1981 gcggtagccg tcacgaatct gcttcagctc ccgcatccgt tctcggatcc gctcaccctg
     2041 cccgtatccc agcagctctg cgtcatttga tgcccttgcg tctgcattat catttttcag
     2101 ggtctcctcc cgggcccgta actgctcccg gattttcttc tggtcaatca gggcagcatt
     2161 gcgcagcagc tcctgcttct gtatctccgt caggattttc agctcgccct gcgaggtctg
     2221 gtacttcagc ttcgccagtt ctgtattctt ccctgccagt gccagttgtt ccttctgctg
     2281 cttcagcaga cgggaaaaac tgtcttccgc tttttccgtc tcagattttc cgccccggga
     2341 tttgggttta ttcgcctcat tattgcgcca ggcttccatc gaattacgga tgtaacgctg
     2401 cctcgcctcc tgatacgtat cccccaccag accaaggtca tccgccgcat accccagccg
     2461 ggcacgctct ttttcttccc ctttcagccg ggacagggcc agttgacgct ctgtgttatt
     2521 cagggagctc tgctgtttat catccagagt ggcctgtggc agtcgcagcg gtacactcgc
     2581 cagtccctga cgctgttgca gcagttcatt acccagcccc agcaggcggt tgaattccgt
     2641 atgctggccg ttcatgacca gcagtgactg atacgcttta ttctgctctg ctgcctgctg
     2701 acgaattaac acaacacggc gctcttccag tccggcaagc acatcctgaa tggactgcgc
     2761 tttttcctgc atctgtgcca gacgggactg ctcaacggca agctgctctg ttgcctgagc
     2821 aagcccttcc gttacagttt tcaccgatgt cagatggttt atcatgaagc cgttatcggt
     2881 cgtccagccc ggattagcca gcacatactg atacccggcg attttttcct gcagggattt
     2941 cacccggctg gcctgctcat caatcagccg gttctgctct gtcagcgcct cccgtgttcg
     3001 tccttcatta tctgaggctt caggcaaaga cattgacggc gttttatgcg cgatttcatc
     3061 aatcgtcagt gcatactggc gcgcagactc catggcctgc tcctgattct ggtacagcgt
     3121 gtaccatgct gctgcaccca gcatgaccag tccggggata ccgccaacca gtcccagcgc
     3181 accgctcatc agacgtgagc ccaccgccgt tgtactgttc agcgcattct gggcggcggt
     3241 tctggcagca atatttctgt tcaggcgttc ctgtgtgacc gccagacggg cctctgccgc
     3301 aatctgcatc tcagtcccgc gggctgccgc cacagcctgc tgagcccggt acacggctgc
     3361 ccttgcccgc gcagtggcaa tctgcgttcc ccttaactgg gcctcagcca gtgccacttc
     3421 attacgtgca gcggtcacaa gtcctgccgt ggcagacatc gctccggaag ccagattacc
     3481 aaagtaccgg gccaccccga cagcaaccag cacacccgca gctgttgcca cggtatcaat
     3541 atttccggcc acaccgttca gcgcgccgga gagcgttttc gtcgctccgc tggcctcatt
     3601 cgcgccgccc acccaggcca taaaggcgtt ttccaccttc gtgatactac cggaaaccgt
     3661 ttccggcatg gctgcatatt catcacgcaa tatccccagc tggctgatta acgcgggaac
     3721 gactttatcc gccgtcagtt gaccatcgtc cgccatcgcc ttcagatcct tacgggccac
     3781 gcccatgcct gcagccagtg cgcgaatgat ccggtcccca ctttcattga cagaattaaa
     3841 ttcctcgccg cgcaacaccc cctgtgccag cgcctggctg aactgggtga tcaccgaacc
     3901 cgcctctgcc gcactggcac cggaaatttt cagccctgtc gaaatggcct ccgtcacctt
     3961 cagtacatca tcagcactgt aaccatattc acgcatcgag gcggcagagc gggcaaacag
     4021 agccgcatta tccgaaaatg cggtgcctgt ccgctggctg atgtccatca gcactttctg
     4081 tgatgacgca aattcatccg atgactgcga cgcctgtttc agacgggcat tcacggagct
     4141 ccattcatcc gccagcgaaa tcaggtgtcc ggtggcaaag gcaccggcaa acgcaccggt
     4201 cattccgaca gctgaagcgc ggatttccgt caactggctg ttcagctcag ccagagcccg
     4261 gcgctgctcc ctggctgccg cagcagcctg acgtccgcca ttctgcaggg tccggtaata
     4321 ttcactgccc atacgggacg ccc
//
LOCUS       DAZGVQ010000269         4301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000269 DAZGVQ010000000
VERSION     DAZGVQ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.272"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..348
                     /locus_tag="ACHLAG_005077"
     CDS             <1..348
                     /locus_tag="ACHLAG_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="HFP0658142.1"
                     /translation="KKNRTTTDEKTLSALIRAEKTAEKAAAAKARVTAIIAAERKAAA
                     RAERKARDHELYKAAGLMIVAGLVDSKTGKPKFSAAELVGALAGIAELPRNHPKWQEW
                     ERRGKELLTKDSA"
     gene            complement(609..1628)
                     /locus_tag="ACHLAG_005078"
     CDS             complement(609..1628)
                     /locus_tag="ACHLAG_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP0658143.1"
                     /translation="MSAALQYFEENLPHRPYHTNDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDSSV
                     KALKYAAAIERSLCEKLCADVNYSGLICKNPFHLEWQVMEWREEAYTLDELADYLDLS
                     ASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLDAVIQRVEMYNASLPV
                     PLSPPECRAIGRSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGKANSSENQSD
                     KGKKSAAVRWTANDDKRRRALDMYILGASTEDIAEAVGVSRWTVRRWMDSSVEWLLSK
                     QIIKI"
     gene            complement(2172..4031)
                     /locus_tag="ACHLAG_005079"
     CDS             complement(2172..4031)
                     /locus_tag="ACHLAG_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HFP0658144.1"
                     /translation="MASYHCTVKTGAKGSALKHADYISRTGEYENYKSREDLEFSSSG
                     NMPSWAKKNPSELWKSADEFERKNGTAYREIEIALPRELTREQRIELVEDFVQKELGD
                     RHAYQYAIHNPPGAIDGKEQPHAHIMFCERINDGIERDPQQFFKRANSKLPERGGAKK
                     ARFGETQQERKSALVELRSRWADVQNEHLARHGHESRVDHRSLKEQGINRTPEVHLGP
                     VQAASLNGEQIVAIQERRNAERELKTARDAANAIQPEQEQKQKIKAVEPVRSARLMDE
                     YRDVARKVITLETRQNESQELALKHHLFMKAEAKEQELAKLKEQLKKMDEQRSVYKNK
                     IDDKKQNITALVEQKKSLERSFIGLFKGGMKRELEEQIKTQVSQLEKTEHEVKQLDEN
                     YQQLQMQADNEQKNFSHSPEYQYSEAVLSGIPRSILKLQFALLPSESTIAFNKNVLDV
                     EQRKEAAIAAELCLSEDERRQIRREIQDVLNGDACEGQQTETLQQKRPEKDAHIEWER
                     EESPQVVIPKTYVFFSACRGVKTEIRPFESCDDAIEWGLVECELHDRFKRDLIVYRVD
                     AELIGKGDDTVMKNAERIDLTDELEGWNQQTHIEKESLFERVQQRSQDDELEM"
BASE COUNT         1052 a         1130 c          991 g         1128 t
ORIGIN      
        1 aaaaaaaacc gaacaacgac ggatgaaaaa acgttgtccg cactgatacg ggcagaaaaa
       61 accgcagaga aagcagccgc agcaaaagcc agggtaacgg caatcatagc ggcagaacgg
      121 aaggcagccg caagggcaga acgaaaggcg cgggatcatg aactctacaa ggccgcaggc
      181 ctcatgattg tggcaggact ggtggacagc aaaaccggaa aaccaaagtt tagtgctgcc
      241 gaactggtag gcgcactcgc aggaatcgca gagctaccgc gcaaccatcc aaaatggcag
      301 gaatgggaaa gacgcgggaa ggagctacta accaaagaca gtgcgtaaga gcctccgttc
      361 acgttccggt tccctggtgc ttcctcgctc attcgctcgc tacgctcggt catgagactg
      421 cggcgagcac aaccacctca atcatgcggt aagccgcacc attgctgggc tgaaaggaga
      481 aaaccgccgg aaagcccgct actcaaaaag acgccgaaag gcgctgttat ctgatatagg
      541 cttgttcatg cacccaaaat taacagaatt ttaacaaaac aggggggggt atgtgccatt
      601 cttgttaatt aaattttaat tatttgttta ctcagtagcc attctacaga gctatccatc
      661 cagcgtcgaa cagtccaacg gctaacacca accgcctctg caatatcttc tgttgaggct
      721 cccaagatgt acatatcaag cgccctacga cgtttgtcat cgttagcagt ccaccgaaca
      781 gcggcgcttt ttttcccttt gtccgactga ttttctgacg aattggcctt cccgcccttg
      841 cgaccgcgtg ctgcctgaat ttctggcgtg tgcgtatcag ccacatactg tgcgaaagtt
      901 tccggcgtga agttcctgtg cgtgtatttc gcaatgctcc tgccaatagc ccgacattca
      961 ggcggagaaa gcggaacggg aagcgatgcg ttgtacattt cgacacgctg aatcacggca
     1021 tcaagccact gtgagaatgc tggccagccc tgacgaatcg ccctgtaagc ccatttgcgc
     1081 gtcatttcaa acaggtggca gtttcgcccc atcccgtaat gtttatcgat gctacgacgc
     1141 tctgaggcgc tcaaatcgag ataatctgcc agttcatcga gggtataggc ttcctcgcgc
     1201 cactccatca cctgccattc caggtggaac ggatttttgc aaatcagtcc gctgtagttc
     1261 acatcagcac aaagtttctc acacagcgaa cgttcaatcg cggcggcata tttcagcgcc
     1321 ttaaccgagc tgtccggcgc ggttctcacg gcgatattga gtgcgtagag caggtgagca
     1381 tgaccgttca caggattttt tacggtgatg ttcggcgcgg gggcgttccg gtcgctccag
     1441 tcaatcgcgg ccccctcgcg gtcaacgtca aaaaccagcc agaactgcgc atgaggctgg
     1501 ttctgctgga tgtaccgcgc aagaagcgca cgccctttgc cggagatgcg aagaccaaaa
     1561 gcgagatcat tcgtgtgata ggggcggtgg ggtaaatttt cttcgaagta ttgaagcgcg
     1621 gcgctcatgt gttacgattc cttccaggtt atcgaccgac atcgcccctg tattttcgtg
     1681 acaatcttgg cggagagaca cgaatttcag gggcgtttgt ttttcagtcg cttacactac
     1741 ccccaacgac aaaacctgtc gagtcttccc gcaaaaccga tccatttacc ataactcccg
     1801 ttgtacgtac cccttaaaac gccctcagtc gctttctgac gcaaaaaacg caaaaagaca
     1861 aggaaacacc agggtaaacc agaaaaatgc gtcagaattg ttttcagggg cctgcataac
     1921 tatgcacaaa agcgattcgc gaacagtttc gaccgttcag aagggcgaac ggggcaaaat
     1981 gacaaacttg ccgttctgaa aaaagcacat tagaacattt ttgagaatgc ttccgttttt
     2041 gtttttctac cgtgcaaaca agtagttagg ttgcggttgc attctcacta tacatcacag
     2101 ccaaacggtg aacgggacgc gaaatccttt cgcggtctgt gaggcgttgg acaagcgaag
     2161 cgcgtcagta actacatctc cagctcatca tcttgtgacc gctgctgtac tcgttcaaaa
     2221 aggctctctt tttcgatgtg tgtttgctga ttccatcctt caagttcatc agtaagatca
     2281 atgcgttcgg catttttcat cactgtatca tcaccttttc ctatcaattc agcatcaact
     2341 cgataaacaa tgagatcacg cttaaaacga tcatgaagtt cacactcaac caacccccac
     2401 tcgatagcat catcgcacga ctcaaacggc ctgatttctg tcttaacgcc cctgcacgca
     2461 gagaaaaaaa cataagtttt tgggatgacg acttgcggag attcttcccg ttcccactcg
     2521 atgtgcgcat ctttctcggg acgtttctgc tggagagttt ctgtctgctg cccttcgcag
     2581 gcgtccccgt ttagaacatc ctgaatctct cgcctgattt gccgccgctc atcttcacta
     2641 aggcaaagtt cggcagctat agcagcttct tttcgttgtt ctacgtccag tacattcttg
     2701 ttgaaggcga tagtgctctc actgggcaac agagcgaatt gcagttttaa aatcgaacga
     2761 ggtattccgc tcaaaaccgc ttccgaatac tgatattccg gtgagtgtga gaaattcttc
     2821 tgttcattat cagcctgcat ctgaagctgc tgataattct cgtccaactg cttcacctcg
     2881 tgctcggtct tttccagttg gctgacctgt gtcttgattt gttcctcaag ctcacgtttc
     2941 atcccgcctt taaacaggcc aataaaactt ctctcgagcg attttttctg ttcaactaac
     3001 gctgtaatat tctgtttttt atcatcaatc ttgttcttat aaacagaacg ctgctcgtcc
     3061 atttttttta gttgttcttt cagtttcgcc agttcttgct ctttcgcttc tgccttcata
     3121 aacaagtgat gttttagcgc cagttcctga ctctcattct gtcttgtctc cagtgtgatc
     3181 actttccggg caacatcccg atactcatcc attaatcggg cagaacgaac aggctcaact
     3241 gccttgattt tttgcttttg ctcctgttcc ggctgaatgg catttgcggc atctctggcg
     3301 gtcttgagtt cccgctcggc attcctccgt tcctggattg caacaatctg ctcaccattc
     3361 agactggcgg cctgaacggg gccaagatga acttccggcg tgcgattgat accctgttct
     3421 ttcagcgaac gatgatctac gcgcgactcg tgaccgtgac gcgcaaggtg ctcattctgc
     3481 acatccgccc agcgggaacg taattcaaca agggcagatt ttcgctcttg ttgtgtttca
     3541 ccaaagcgtg ccttttttgc gcctcctctc tccggtaatt tgctgttagc acgtttaaaa
     3601 aactgctgcg gatcgcgttc aataccatca ttaatccgct cgcaaaacat gatatgcgcg
     3661 tgtggttgtt cctttccgtc aattgcacct ggcggattgt ggatcgcgta ctggtaggca
     3721 tggcgatcgc caagttcctt ttgcacaaaa tcttcaacaa gttctatccg ttgttcgcgt
     3781 gtcagttcac gcggtaacgc gatctcaatt tcacggtatg ccgtcccgtt ttttcgttca
     3841 aattcatctg cggatttcca tagctcggag ggatttttct tcgcccatga tggcatgtta
     3901 ccggatgacg aaaactccag atcctcccgc gatttatagt tctcgtattc gccggttctg
     3961 gaaatgtaat cagcatgttt taaggcactg ccttttgcac cagttttaac ggtgcaatgg
     4021 taactagcca tctgcctttt agccctccgc aggagcgcac agtttctcga cgaaggagag
     4081 aaacgtctaa gtgcgcatcc ctgaattaat cagggatgat tttacggtct cacacgcagg
     4141 cagacaaggt gatattatgc acaataacaa ctcacattaa cggcaaaagt gcagaggtaa
     4201 gaatgacaaa tcgtgaagag tggttaagtg caaaaattgc ctacatcaac ggactgaaat
     4261 caccgtcaga gcagcagaga ttacttgtgt tactagcaga g
//
LOCUS       DAZGVQ010000270         4160 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000270 DAZGVQ010000000
VERSION     DAZGVQ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.273"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            41..1699
                     /gene="dpiB"
                     /locus_tag="ACHLAG_005080"
     CDS             41..1699
                     /gene="dpiB"
                     /locus_tag="ACHLAG_005080"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFP0658145.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1668..2348
                     /gene="dpiA"
                     /locus_tag="ACHLAG_005081"
                     /gene_synonym="citB"
     CDS             1668..2348
                     /gene="dpiA"
                     /locus_tag="ACHLAG_005081"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFP0658146.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2389..3774)
                     /gene="dcuC"
                     /locus_tag="ACHLAG_005082"
     CDS             complement(2389..3774)
                     /gene="dcuC"
                     /locus_tag="ACHLAG_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP0658147.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AGAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1151 a          967 c         1059 g          983 t
ORIGIN      
        1 catccgtcaa agaggctcgg gacaacccgc aaggaaaaca atgttgcagc ttaacgagaa
       61 taaacagttt gcatttttcc aaagactggc atttccgctg cgtatctttt tgctgattct
      121 ggtgttctca atatttgtca ttgcagccct ggcgcaatat tttacggcca gttttgagga
      181 ctatttaacg cttcatgtac gcgacatggc aatgaatcag gcgaaaatta ttgcctccaa
      241 tgacagtgtc atctctgcgg tgaaaacgcg tgactacaaa cggctggcga ccatcgctaa
      301 caaattacaa agagataccg attttgatta tgtggtgatt ggggaccggc actcgatccg
      361 cctttaccat cctaatccgg agaaaattgg ttatcctatg cagttcacca aacagggcgc
      421 gctggagaaa ggggagagct acttcattac cgggaaaggg tcaatgggga tggcgatgcg
      481 cgccaaaacg ccaatctttg atgacgatgg aaaagtcatc ggcgtggtgt cgattggcta
      541 cctggtgagt aaaatcgata gctggcgggc tgagttttta ttaccgatgg caggcgtgtt
      601 tgtcgtgctg ttagggattc tgatgttgct gtcgtggttc ctggcagcgc atatccgtcg
      661 gcaaatgatg ggcatggagc caaagcaaat cgcacgcgtg gtccgtcagc aagaggcgct
      721 gtttagttcg gtttatgaag ggctgattgc ggtggatccg catggttaca ttaccgccat
      781 caatcgtaac gcaagaaaga tgctggggct gagctctccc ggacggcaat ggttgggtaa
      841 acccattgct gaagtggtca ggcccgccga tttctttacc gaacagattg atgaaaaacg
      901 tcaggatgtg gtggctaact ttaacggtct gagcgttatt gccaatcggg aagctattcg
      961 ttcaggtgat gatttgctgg gggccattat cagctttcgt agtaaagacg aaatttccac
     1021 cctcaatgcg caactgacgc aaataaaaca atacgtcgag agccttcgta cattgcgaca
     1081 cgagcatctc aattggatgt cgacgctcaa tggtctgttg cagatgaaag agtatgatcg
     1141 cgtgctggcg atggtgcagg gggagtctca ggcccagcaa cagcttattg acagcctgcg
     1201 cgaggcgttt gccgatcgcc aggtggcggg gctgcttttt ggtaaagtgc agcgcgcccg
     1261 ggaactgggg ctaaaaatga tcattgtccc cggtagccag ctttcgcaac tgccgccagg
     1321 actggatagc accgagtttg cagccattgt gggcaattta cttgataacg ccttcgaagc
     1381 cagcctgcgt agcgatgaag gaaacaagat cgttgaatta ttcctcagcg atgaaggcga
     1441 tgatgtggtg attgaagtcg ccgatcaggg ctgcggcgtt ccagagtctc tacgagacaa
     1501 aatatttgag cagggggtca gtacgcgtgc tgacgagccc ggtgaacatg gcattgggtt
     1561 gtacttgatt gccagctacg taacgcgctg cggtggtgtt atcactctcg aagataatga
     1621 tccctgcggt accttatttt caatctatat tccgaaagtg aaacctaatg acagctccat
     1681 taaccctatt gatcgttgag gacgaaacgc cgctggcaga gatgcatgcg gaatatattc
     1741 gtcacattcc cggattcagt cagatattac tggcgggaaa cctggcgcag gcccgaatga
     1801 tgatcgagcg ttttaagccg gggctaatct tgctcgataa ctatcttcct gacggcagag
     1861 ggattaattt attgcatgaa ctggtgcagg cgcattatcc cggcgacgtg gtgtttacca
     1921 ctgcagccag cgatatggaa acggtgtctg aagccgtacg ttgtggtgta tttgattatc
     1981 tcattaagcc cattgcctat gaacggctgg ggcaaacgct aacccgtttc cgccagcgta
     2041 aacatatgct ggaaagtatt gatagcgcca gccagaagca aattgatgag atgtttaatg
     2101 cctatgctcg cggtgaacct aaagacgaac tgccgaccgg cattgatccc ttgacgctaa
     2161 acgcggtgcg aaaactgttt aaagagcctg gtgtgcaaca tacggcagaa acggtggcgc
     2221 aggcactgac catcagtcgc accactgcca ggcgttatct tgaatattgc gccagccgcc
     2281 atctgattat tgctgaaatt gttcacggca aagttggcag accgcaacgc atataccaca
     2341 gtgggtgaca tgataaagcc ggaggaaact tgcctccggc attactattt acttgcctgt
     2401 gaccgctgct gccgttcctg gcaccatcag ctctgtagca acgataacaa tcaccaaacc
     2461 aacaagcacc ggtaccgagg tgcgttttac gacttcaaac ggcgagatct tcgccatccc
     2521 ggcaaccgca acgactacgc cagaaacggg tgatagggta cgacccaggt ttgacgcctg
     2581 cagcatcggg atagtcaaat acgccgggtt aatgccggaa gagtgcgcca gtttcgggat
     2641 catctcaaca aacgcataaa acggcgcatt gcctgaaccg gtcgtgactg ccgccagcat
     2701 ggtcagaatc accaatacca gcatcaggat gatactcgcc gaaccaaacg aggtagcgat
     2761 agaaatcaga ctttgaataa agccgatggt gctaagcccc tgagcgaata ccccagcggc
     2821 aaccagcagc atcaccacgt tagcaaacgc atctgccatc ccgcgataag ccacttccag
     2881 accagagaaa actttctggg tattaaagct gcggaggaac tccagaatgg aggcaatcag
     2941 catacaaatc accagaatag tgatgatgtg taattgcgga ccccatttac cgtcaaaaat
     3001 cagtacaccg atgatcggcg tgaacggcaa aatggcataa aacgcaggag cagtggtggt
     3061 gatttcactg acatctaaca tttcatgaga gatgtgctct tttttatcca gataacgttg
     3121 ccagaagaag tgggcgatcg ccatgccgat aattgcagca attgagatag gcagcgtcgt
     3181 tttgaaggcg aagtcaatca gcgacatttc ggaagcttgc gccgccagca ccacatcccc
     3241 tgaagtcggt gcgagaataa tcgccgccgg ggaggcacaa atggcagcac ctgcgccacg
     3301 actgatacca acgtttacca tcaccggaaa tagggttgcc atcagcaaaa cacccagacc
     3361 ggttgcggaa gagacggcca gagacatcag acaggcgaca aaataggcgg caatcatcag
     3421 caggtaaggg gagttaatat actgcaatgg ttttgacgcc agcttgacca ccatatcatt
     3481 cgcgccgata tgggtcatgt aagcggcaaa tccacacagc atcataatca tcatgccgag
     3541 gtcgccgccg cggctcatta gtaatatttt aacgtattca acgatatccg tggcgctgta
     3601 gcctgttgaa gcctggctgg acggtaacac tttgtgcccc ataatggcac tgataatcag
     3661 caataacagg ccaccgacaa ataacacacc agtggcggaa taccctttaa tgatgtagcg
     3721 agctacaccc acaataacca caaccccaat aaggagttca atgaatgtca gcataatttt
     3781 tcctgtctcc aggccccaaa gtaaataata aaaaattcct aaagcttaag gaaaaaatat
     3841 gcccaataaa ttggcgatga atgctgatta aaatcaagaa aaactgccat taagacattg
     3901 aagttgctgt ttttatacat agataacaaa acgcatattt agcggttggc aattatcaca
     3961 taataatgtg tgcaaattga tggtggattg atgcaaattt gttaattgca atggtgttag
     4021 ctgcggctgt gcgctcaaaa aataatctaa tatgagcata ggttgacgat atatattata
     4081 tacttcgtta agatgattgt tgtatctcgt taaaaaataa aataattttt ccttgattgc
     4141 attttgtcat caaaaaagac
//
LOCUS       DAZGVQ010000271         4123 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000271 DAZGVQ010000000
VERSION     DAZGVQ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.274"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..420)
                     /locus_tag="ACHLAG_005083"
     CDS             complement(<1..420)
                     /locus_tag="ACHLAG_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658148.1"
                     /translation="MKIYWTRKSIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILFRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILER
                     GKETETISERLIREADTREYELWRPARRFFSIVFLIVL"
     gene            878..1387
                     /locus_tag="ACHLAG_005084"
     CDS             878..1387
                     /locus_tag="ACHLAG_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658149.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
     gene            1476..2114
                     /locus_tag="ACHLAG_005085"
     CDS             1476..2114
                     /locus_tag="ACHLAG_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP0658150.1"
                     /translation="MRTRIDYLADKYSFTELNERPHLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLIRTPQLIAALREEWDIGQKNVLFNDKRFGCVYSLKS
                     SLSGVPDTYRYHLSHRIRRVVANENTSLPYQQIAREVKAPRERLKYALETGLQVTALD
                     GLFWFGSQRIAADIQRLRKSGMRITTSAISVYDSITVTRRLCPVYRIVANNK"
     gene            2451..4070
                     /locus_tag="ACHLAG_005086"
     CDS             2451..4070
                     /locus_tag="ACHLAG_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP0658151.1"
                     /translation="MARIPTKKAKAKKGFEETLWDAANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFETRRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNAKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPANTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTKDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTAEDIATIADTYHAWRSTPEELA
                     ARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRQNLEALGYGE"
BASE COUNT         1142 a          889 c         1019 g         1073 t
ORIGIN      
        1 tagaacaatg agaaaaacaa tggagaaaaa gcgacgggcc ggacgccata gttcatattc
       61 tcgtgtatca gcctcacgta tcaacctttc tgaaatggtt tcggtttcct tacctcgttc
      121 cagaatatgt ctgtaatatt tcctcatgac atataccgaa aactgaaacc agattaatat
      181 ggatggacac acaacacata aacttctgat gatatccccc gggaaagtat cttgggcggg
      241 taaaagaaag tcatatactc tgaataaaat catcatcgag ataaatgaga cgccagcacc
      301 tatccagtat tcgatatggg aagatgcagc attatatgcg tcagtaaaat ttttctttct
      361 taatgaaggt ggaagtgctg aaagttcagg aatgcttttt cttgtccagt atattttcat
      421 atggcaactc atataaaagt gttcatactt tactatgatg atgacgattt ataaaaggct
      481 tataatgact acttctcctt tcattctcga atttcacgat aatgatagag ataatcatta
      541 tcaattgatt gtgtcagtaa ttttgactta catcacgctt tcatggttta atcaggtgat
      601 tgatttaatg aaaagagtgt gtttgtggat gtattgtgta tatgtcttac cgggcatctg
      661 ttcttacatt aattaatata ttgctattta agtattggtg gtattgtcag taacattctt
      721 ttggttaatt tagttattat tcctcactga ttaattgtcc ttccgttctt attgtttgta
      781 acttgtgttt gtatcgtacc tttcaggtgt tgatgcgcgc tttactgtgt ctgcaccggc
      841 ttgacaaaat tcaacagggt ttgaaaagga acatttcgtg caaataaccg aagccttaat
      901 ttcagagccg ggggaaatcc ggcgttttgt tcagcaggcg gtggaccact ggccaaatct
      961 gttggcattt cacttcacgc tctattcggc agaaggaatc tatggtcaac agattcagac
     1021 attttgtagt tcctttcacc gacgggtaca cgaacgtatt acggagcata atcacacggt
     1081 cagcccatca gcgccggtgg tattacgctg gttgcgggaa caacatgaag gagcacagat
     1141 acgatgtctg ttgctgctca gccagacgag tatttgccat ccgcgagtcg gcgtcatggc
     1201 tgatgaagaa tgtgcgcaac tggtggattt actgcaacag acctggtcgg tgataagtgc
     1261 tggcggacaa tgccgggtgg aaagatgttt tcgggttgcc cggcctggtt catcagggca
     1321 atacgtggcg ttaaaaacgg ctgttcagtc ctttatgtcg caggttattg caacaatcat
     1381 ccggtgattg tcgggaggaa tatcaatgct tccgttaatt tttactggct gataaagttt
     1441 tgtctgctga atctttatac ggaattcctt tcagtatgcg tacacgaatt gactatcttg
     1501 cagataaata cagttttact gaactgaatg aacgtccaca ccttcgccgg cagtggcagg
     1561 atgttctgga ggagtgtcgg cagacagagg ccgggccaga agaacggctg cggattgccc
     1621 tgttgaatgt ggattacgtc accagttttg aacttccatt tcggttgctg cttatccgta
     1681 caccacaact gattgctgcg ttgcgtgaag aatgggacat cggccagaaa aatgtgctgt
     1741 ttaacgataa acggtttggt tgcgtgtaca gcctgaagtc cagtctttct ggtgtaccgg
     1801 atacataccg gtatcatctg tctcaccgta ttcgtcgtgt ggttgcgaat gaaaacacat
     1861 cattgccata tcagcagatt gcccgggaag tgaaagcccc ccgggaacga ctgaagtatg
     1921 cgctggagac gggattgcag gtgaccgcac tggacggctt gttctggttc ggtagccagc
     1981 gcattgctgc agatatacag agactgagaa aatctggtat gaggattacc acttctgcca
     2041 taagcgtgta tgactctatt accgtaaccc ggagattgtg cccggtatac cggattgtcg
     2101 cgaataataa ataaacggta atgctgagtt tctccattac cattgcaaat gactcaccag
     2161 agcagactga acagcgcaga agtgggattg tggatacgtg aagtgagagt aaggggaaaa
     2221 tccacaataa tcatctatcg aacagggagg cgaactttac acgatggttt tccgggagtg
     2281 cttacccggg gttcctcacc tctggctaat ctctggattg agtcgcgata ctccaacaaa
     2341 agcaacaagc taacgcagca agatgttaac gctcatcgag agtaaaatgc acacttttat
     2401 ggcttactcg ttacaataac agccagtttg ttcagaaaac cggattcagt atggccagaa
     2461 taccaaccaa aaaagctaaa gcaaaaaaag ggtttgaaga aacattatgg gatgccgcaa
     2521 atcagcttcg cggcagcgtt gagtcctccg aatacaagca cgtggtgttg agcctcgtgt
     2581 tcctgaaatt catcagcgat aagtttgaaa cacgccgcaa aaaaatgatt gccgatgggc
     2641 aggcagattt ccttgagatg gaagtgttct accagcagga caacattttc tacctgccgg
     2701 aagaggcgcg ttggtcattt atcaaacaaa atgcaaaaca ggacgatatt gcggttcgta
     2761 ttgacaccgc cctctcgacc attgagaaac gtaacccaac cctgaaaggt gcgctgccag
     2821 acaactactt cagccgtcag aatctggaaa ccaaaaaact ggcatcactg attgatacca
     2881 tcgacaacat cgaaacgctg gcacacgaga ctgacgttga aacgttatcg aaagaagacc
     2941 tggtcggacg cgtttatgaa tacttcctcg gtaagtttgc cgccactgaa ggcaaaggcg
     3001 gtggtgagtt ctacacgcca aaatgtgtgg tcacgctgtt aactgaaatg ctcgaaccct
     3061 tccagggcaa aatttatgac ccgtgctgcg gctcggcagg aatgttcgtg cagtcggtga
     3121 agtttgtcga gagccatcag ggtaaaagcc gtgatatcgc gctgtatggt caggagctga
     3181 cagccacgac gtataaactg gcaaaaatga acctcgctat tcgcggtctt tcagctaacc
     3241 tcggcgaacg cccggcaaac actttcttta gcgaccagca cccggacctg aaagctgact
     3301 atattctggc gaacccgccg ttcaacctga aagactggcg taacgaagca gaattaacca
     3361 aagacccacg ttttgccggt tatcgtatgc cgccaaccgg taacgccaac tacggctgga
     3421 ttttgcatat gctctccaag ctgtcggcta acggcacagc gggttttgtg ctggcaaacg
     3481 gttcgatgag ttctaacacc agcggtgaag gcgagatccg tgcacagatg atcgaaaatg
     3541 atctgatcga ctgcatgatt gctctgccag gtcagttgtt ttacaccacg cagatcccgg
     3601 tgtgtttatg gtttatgacc aaatcgaagg ctgccgatcc ggccaaaggt tatcgtgatc
     3661 gtcagggcga gacgctgttt attgatgcgc gtaacctcgg caccatgatt agccgcacaa
     3721 ctaaagagtt aacagcggaa gatattgcca caatcgccga tacttaccat gcctggcgta
     3781 gcacgccaga agaactggct gcacggattg cgcgtggtga cagcaagctg gaaaaatatg
     3841 aagaccaggc aggcttctgc aaagttgcga ccctgcaaga tattaaagat aacgactacg
     3901 ttctgacacc gggccgctat gtgggtgcag ccgagcagga agaagacggc gtggcatttg
     3961 agaccaaaat gcgtgaattg tcgaagacgt tgtttgagca gatgaagcag gcggaagaac
     4021 tggatcgtgc gattcgccag aatctggagg cgctgggtta tggggagtaa atgggagaaa
     4081 ataaaactta aagaagttgt agatattatc actactaaag ttg
//
LOCUS       DAZGVQ010000272         4112 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000272 DAZGVQ010000000
VERSION     DAZGVQ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.275"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..258
                     /locus_tag="ACHLAG_005087"
     CDS             <1..258
                     /locus_tag="ACHLAG_005087"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658152.1"
                     /translation="LKKDKMNLCVPSFPSEHIHYSNFTQPRTNKKHHPGYCRYRFPAG
                     KNHKQNTGNNAFRNPPLLKKNKIKKTTVPALNAEYNILWTG"
     gene            1096..1968
                     /locus_tag="ACHLAG_005088"
     CDS             1096..1968
                     /locus_tag="ACHLAG_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP0658153.1"
                     /translation="MNPSDAIEAIEKPLSSLPYPISRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            2296..>4112
                     /locus_tag="ACHLAG_005089"
     CDS             2296..>4112
                     /locus_tag="ACHLAG_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFP0658154.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKGAGVAVALSLAA
                     VTSVPALAADTVVQAGETVSGGTLTNHDNQIVFGTVNGMTISTGLEYGPDNEANTGGQ
                     WVQDGGTANKTTVTSGGLQRVNPGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHE
                     GAIATGTVINDKGWQVVKPGTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINK
                     NGRQIVRTEGTANTTVVYAGGDQTVYGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQ
                     IVKNGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADSG
                     AAVSGTRSDGKAFRIGGGQADALMLGKGSSFTLNAGDTATDTTVNGGLFTARGGTLAG
                     TTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSNTTLT
                     QKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASSATWNIP
                     DNATVQSVVDDLSHAGQIHFTSARTGKFVPTTLQVKNLNG"
BASE COUNT         1100 a         1086 c         1046 g          880 t
ORIGIN      
        1 ctgaagaaag acaaaatgaa tctgtgtgtc ccctccttcc cttcagagca catccactat
       61 tcaaatttta cacaaccacg tacgaacaaa aagcatcatc ccgggtattg cagataccgg
      121 tttcctgccg ggaagaatca taaacagaac accgggaata acgccttcag gaatcccccc
      181 ctcctgaaga agaataaaat aaagaagact acagttcctg cactgaatgc tgagtacaat
      241 attctatgga caggatgaca tatttataaa taacaaaaac catttaatac tgatacatca
      301 ataaaatact tcctccctgc tgacgcagat aaaatgcacc attttttctt caaatacttc
      361 tgtataatcg ccagccaatg atgagaatgg attaaaataa cacttcttcg acaaacacaa
      421 aaacagcctt cattttacat catcatccag tttcagttaa ccccggacga gcgacagcgt
      481 ttcagtacag gaattaaaag ccagcttttg ctcagtaaaa acacactcaa ccaataatat
      541 ctcagcagat attatcctga ccaggacacc tcacattact gaattctctt ccgacagtat
      601 cactgcacaa ataaaaaaga acgggataaa tattcagacc ggaagaatgg tgtcacaaaa
      661 gcatttaatc attacgcatg accaggaagg gaaacactta accacatggt atcaacagat
      721 atcattacgt gtgcactctt ataaaaaaca cacagaagaa gtaagataat tataaacgta
      781 aatttaatat ctcccactga catctccccc acaatacact gaaaaatact cacagcagta
      841 aacacagaca catctgatag tcatgcagcc gataaatatt ttccccttta cggagaaatc
      901 agcctgctgt ttaacaataa cggcgtattt tcatggaagg agatcaccca gtaacggata
      961 agtcctgatc tccttcatgt taatacgcaa caatcccatc gattcaaaac aatttcagac
     1021 agatattatc tccgtgaatc ccccgccacc tttccggtgc gcggggtttt gtcttttttc
     1081 accgggaata catgtatgaa tccgtctgat gccattgagg caattgaaaa accgctctcc
     1141 tccctgcctt acccgatttc ccgtcacatc ctggaacatc tgcgcaaact cacccgtcac
     1201 gaacccgtga ttggcattat gggtaaaagc ggggccggta aatcctcact ctgtaatgcg
     1261 ctgtttcagg gggaggtcac accggtcagt gatgttcacg ccggcacccg ggaagtgcag
     1321 cgcttccgtc tgagtggtca cggtcacagc atggttatca ctgacctgcc cggggtgggc
     1381 gagagccggg acagggatgc agagtatgaa gccctgtacc gtgacattct gcctgaactt
     1441 gacctggtac tgtggttgat taaagccgat gaccgtgccc tgtctgtgga tgagtatttc
     1501 tggcgacaca tcctgcaccg gggacatcag caggtgctgt ttgtggtgac gcaggccgac
     1561 aaaacggagc cctgccatga atgggatatg gccggtattc agccttctcc tgcacaggca
     1621 cagaatattc gcgaaaaaac ggaggcggta ttccgtctgt tccggcccgt acatccggtt
     1681 gtggccgtat cggcccgcac cggctgggaa ctggatacgc tggtcagtgc gctcatgaca
     1741 gcgcttcccg accatgccgc cagtcccctg atgacccgac tgcaggacga gctgcgcacg
     1801 gaatctgtcc gctctcaggc ccgtgaacag tttaccggtg cggtggaccg gatatttgac
     1861 acagcggaga gcgtctgtgt tgcctctgtt gtccgtacgg ccctgcgcgc tgttcgtgac
     1921 acggtggtct ctgttgcccg tgcggtatgg aactggattt tcttctgaac ctgtcgtgac
     1981 tgatgccctc cctgactctg aatctgctca caaaagcgct gttttcgtta ctgtctctct
     2041 tgttcgtgca atagctcaac aatagaataa aacgatcgat atctatttta tcgatcgttt
     2101 atatcgatcg ataagctaat aataaccttt gtcagtaaca tgcacaaata cgcacagaaa
     2161 gacattcagg gaacaacaaa accacaattc agaaactccc acagccggac ctccggcact
     2221 gtaacccttt acctgccggt atccacattt gtgggtaccg gcttttttat tcaccctcaa
     2281 tctaaggaaa tgctgatgaa acgacatctg aacaccagct acagactggt atggaatcac
     2341 attacgggta ccctggtggt ggcttccgaa ctggcacgct cacggggaaa aggcgccggt
     2401 gtggcggttg ctctgtctct tgctgctgtc acgtcagtcc cggcactggc tgctgacacg
     2461 gttgtacagg cgggagaaac cgtgagcggc ggaacactga caaatcatga caaccagatt
     2521 gtcttcggta cggtcaacgg aatgaccatc agtaccggtc tggagtacgg gccggacaat
     2581 gaggccaata ccggcgggca atgggtacag gatggcggaa cagccaacaa aacgactgtc
     2641 accagtggtg gtcttcagag agtgaacccc ggtggaagtg tctcagacac ggttatcagt
     2701 gccggaggcg gacagagcct tcagggacgg gcagtgaaca ccacgctgaa tggtggcgaa
     2761 cagtggatgc atgagggggc gatagccaca ggaaccgtca ttaatgataa gggctggcag
     2821 gtcgtcaagc ccggtacagt ggcaacggat accgttgtta ataccggggc ggaaggggga
     2881 ccggatgcag aaaacggtga taccgggcag tttgttcgcg gggatgccgt acgcacaacc
     2941 atcaataaaa acggtcgcca gattgtgaga actgaaggaa cggcaaatac cactgtggtt
     3001 tatgccggcg gcgaccagac tgtatatggt cacgcactgg ataccacgct gaatggggga
     3061 taccagtatg tgcacaacgg cggtacagcg tctgacacgg ttgtgaacag tgacggctgg
     3121 cagattgtca aaaacggggg tgtggccggg aataccaccg ttaatcagaa gggcagactg
     3181 caggtggacg ccggtggtac agccacgaat gtcaccctga agcagggcgg cgcactggtt
     3241 accagtacgg ctgcaaccgt taccggcata aaccgcctgg gagcattctc tgttgtggag
     3301 ggtaaagctg ataatgtcgt actggaaaat ggcggacgcc tggatgtgct gaccggacac
     3361 acagccacta atacccgtgt ggatgatggc ggaacgctgg atgtccgcaa cggtggtgcc
     3421 gccaccaccg tatccatggg aaatggcggg gtactgctgg ccgattccgg tgccgctgtc
     3481 agtggtaccc ggagcgacgg aaaggcattc cgtatcggag gcggtcaggc ggatgccctg
     3541 atgctgggaa aaggcagttc attcacgctg aacgccggtg atacggccac ggataccacg
     3601 gtaaatggcg gactgttcac cgccaggggc ggcacactgg cgggcaccac cacgctgaat
     3661 aacggcgcca tacttaccct ttccgggaag acggtgaaca acgataccct gaccatccgt
     3721 gaaggcgatg cactcctgca gggaggctct ctcaccggta acggcagcgt ggaaaaatca
     3781 ggaagtggca cactcactgt cagcaacacc acactcaccc agaaagccgt caacctgaat
     3841 gaaggcacgc tgacgctgaa cgacagtacc gtcaccacgg atgtcattgc tcagcgcggt
     3901 acagccctga agctgaccgg cagcactgtg ctgaacggtg ccattgaccc cacgaatgtc
     3961 actctcgcct ccagtgccac ctggaatatc cccgataacg ccacggtgca gtcggtggtg
     4021 gatgacctca gccatgccgg acagattcat ttcacctccg cccgcacagg gaagttcgtt
     4081 ccgacaaccc tgcaggtgaa aaacctgaac gg
//
LOCUS       DAZGVQ010000273         4060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000273 DAZGVQ010000000
VERSION     DAZGVQ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.276"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            77..685
                     /locus_tag="ACHLAG_005090"
     CDS             77..685
                     /locus_tag="ACHLAG_005090"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFP0658155.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            783..2174
                     /gene="selA"
                     /locus_tag="ACHLAG_005091"
     CDS             783..2174
                     /gene="selA"
                     /locus_tag="ACHLAG_005091"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFP0658156.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2171..4015
                     /gene="selB"
                     /locus_tag="ACHLAG_005092"
     CDS             2171..4015
                     /gene="selB"
                     /locus_tag="ACHLAG_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFP0658157.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
BASE COUNT          907 a         1027 c         1202 g          924 t
ORIGIN      
        1 caaaccatca acattttctg ccctttttcc catcaccagg ccatactgat atttttcctc
       61 gcacaaagga ctcggcatga aactcgtcgg tagctacacc agcccgtttg tacgcaaact
      121 ttctattctg ttgttagaaa agggcataac tttcgaattt attaatgaac tgccctataa
      181 cgcggacaac ggcgtggcgc aatttaaccc gttaggaaaa gtgccggtgc tggtgaccga
      241 agagggcgaa tgttggtttg attcgccgat catcgctgaa tatattgaat taatgaatgt
      301 cgctccggcg atgttgccgc gcgatccgct ggagtcgttg cgggtgcgca aaattgaggc
      361 actggcggat ggcattatgg atgccgggct ggtatcggtg cgtgaacagg cgcgtccagc
      421 ggcgcagcag tctgaagatg aattgttacg ccagcgggag aaaatcaacc gcagtctgga
      481 tgtgctggaa ggatatctgg tcgatggcac actcaaaacc gatacggtca atctggcgac
      541 tatcgccatt gcctgtgccg tcggatatct caatttccgc cgcgttgcgc caggctggtg
      601 tgtcgatcgc ccacacttag tcaaactggt cgaaaacctg tttagccgcg aaagttttgc
      661 tcgcaccgaa ccgccaaagg cttgatgcgc gatatgtcct cctgacccat ctcacgttac
      721 aatccgtggt tatgttaaac gcccttctcc gtgtgagagg gccttgatca gccaggtttc
      781 ctatgacaac cgaaacgcgt tccctctata gtcaacttcc ggctattgat cgcttattgc
      841 gcgatagctc cttcctttct ttgcgtgata cttatggtca cacccgcgtg gtggaattgt
      901 tgcgtcagat gctcgacgaa gcgcgagaag tgattcgtgg cagccagacg ctgcctgcgt
      961 ggtgtgaaaa ctgggcgcaa gaagtcgatg cccggttgac gaaagaagcg cagagtgcgc
     1021 tgcgtccggt gatcaacctg acgggaaccg tgctgcatac caaccttggg cgagctttac
     1081 aggcggaagc cgcggtggaa gccgttgcgc aggctatgcg ttcgccagtg accctcgagt
     1141 acgatctgga cgacgctgga cgcggacatc gcgatcgtgc gctggcgcag ttgctgtgcc
     1201 gcattacggg ggcggaagat gcctgtatcg ttaataacaa tgcggcggcg gtgttattga
     1261 tgttggcggc cactgccagc gggaaagagg tggtggtttc tcgcggcgaa ctggtggaga
     1321 ttggcggcgc gtttcgtatt cccgatgtta tgcgtcaggc aggctgcacc ctacacgaag
     1381 tagggaccac caaccgcacg cacgcgaatg attatcgtca ggcggtgaat gaaaataccg
     1441 cactgttgat gaaagtacat accagtaact acagcattca ggggttcacc aaagcgatag
     1501 atgaagcgga actggtggcg ctcggcaaag agctggatgt tcccgtagtg actgatttag
     1561 gcagtggctc gctggtcgat cttagccagt acggtttgcc gaaagagcca atgccgcagg
     1621 agttgattgc ggcgggcgtc agtctggtga gtttctccgg cgacaagttg ttaggcgggc
     1681 cgcaggcagg aattattgtt ggtaaaaaag agatgatcgc ccgcctgcaa agccacccgc
     1741 tgaagcgtgc attacgcgcg gataaaatga ccctcgcggc gctggaagcc acgttgcgtc
     1801 tttatttaca ccctgaagct ctgagtgaaa aattaccgac cctgcgcctg cttacccgca
     1861 gcgcagaggt cattcaaatc caggcacaac gtttacaggc cccccttgcc gcacattacg
     1921 gcgcggagtt tgcggtacag gttatgccat gtctttcgca gattggcagt ggttcgctgc
     1981 cggttgatcg cctgccgagc gcggcattaa cgtttacacc ccatgatgga cgcggtagcc
     2041 accttgagtc attagccgcc cgctggcgtg aattgccagt gccggtgatt ggtcgtattt
     2101 atgacggacg attgtggctg gatttacgct gccttgaaga tgagcaacgg tttttggaga
     2161 tgttgttgaa atgattattg cgactgccgg acacgttgac cacggcaaaa caaccttatt
     2221 gcaggcgatt actggcgtaa atgctgaccg tctgccggaa gaaaaaaagc gcggcatgac
     2281 catcgatctc ggctatgcct actggccgca gccggatggt cgcgtgcctg gttttatcga
     2341 cgtacccggt catgaaaagt ttctttccaa catgctggcg ggcgttggtg gtatcgatca
     2401 cgcgctgttg gtggtggcgt gcgatgacgg cgtgatggca cagacccgtg agcatctggc
     2461 gattttgcag ctgaccggta acccgatgct gacagtggcg ctgaccaaag ccgatcgcgt
     2521 ggacgaagcg cgtgttaatg aggttgaacg ccaggtaaag gaggtgctgc gggaatacgg
     2581 ttttgctgag gcaaaactgt ttatcaccgc agcaaccgaa ggtcggggaa ttgatgccct
     2641 gcgcgagcat ctgcttcagt tgccggaacg cgagcacgcc agccaacata gtttccgcct
     2701 cgctattgac cgcgcattta ccgtaaaagg tgccgggctg gtcgtcaccg gtacggcgtt
     2761 aagcggggaa gtgaaggtag gcgattcact ctggctgact ggtgtaaata aaccgatgcg
     2821 tgtacgtgcg ctgcatgcgc aaaaccagcc aacagaaacc gctaatgccg ggcagcgtat
     2881 cgcgcttaac atcgcgggtg atgcggaaaa agagcagatt aaccgtggcg actggctgct
     2941 tgccgatgtg ccgccagagc cgttcacacg ggtgattgtc gagcttcaaa cccatacacc
     3001 gctgacccag tggcagccgc tgcatattca ccacgccgcc agccacgtta cgggacgcgt
     3061 ttcgctgctg gaagataacc ttgccgagct ggtgttcgac acgccgctgt ggctggcgga
     3121 taacgatcgc ttagtgctgc gcgatatctc ggcccgcaac acgctggcgg gggcgcgtgt
     3181 ggtgatgctt aacccgccgc gtcgcggtaa acgtaagccg gaatatctgc aatggctggc
     3241 gtctcttgca cgggcgcaga gcgatgccga tgcgttatct gttcatctgg aacgcggcgc
     3301 ggttaacctt gcggatttcg cctgggcgcg ccagctcaac ggcgaaggga tgcgcgaatt
     3361 gctgcaacag cctggctata ttcaggctgg ttatagcttg ttgaatgcgc cggttgccgc
     3421 tcgctggcaa cggaaaattc tcgacacatt agcgacttat catgagcaac atcgcgatga
     3481 acctgggcct gggcgcgaac gtctgcgacg tatggcgttg ccaatggaag atgaagcgct
     3541 agtactgttg ctgattgaaa agatgcgcga aagcggcgac atcctcagtc atcacggttg
     3601 gctgcatctg ccggatcaca aagcgggctt cagcgaagag cagcaggcca tctggcaaaa
     3661 agcagagccg ctgtttggtg acgagccgtg gtgggtgcgt gatctggcaa aagagacggg
     3721 taccgacgag caggcaatgc gcctgacgct acgccaggcg gcgcagcaag gaataattac
     3781 cgcgatcgtc aaagatcgtt attaccgtaa cgatcggatt gtcgaatttg ccaatatgat
     3841 ccgcgatctc gatcaggagt gtggttcaac ctgcgcggcg gatttccgcg atcgcttagg
     3901 cgtaggccga aagctggcaa ttcagattct ggaatatttt gaccgcattg gctttacgcg
     3961 tcgtcgtgga aatgatcatt tattgcgcga cgcattatta tttccggaaa aataaggaaa
     4021 tgattaatta atttttaaaa taaattaata caaatttctt
//
LOCUS       DAZGVQ010000274         4038 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000274 DAZGVQ010000000
VERSION     DAZGVQ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.277"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..759
                     /locus_tag="ACHLAG_005093"
     CDS             <1..759
                     /locus_tag="ACHLAG_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFP0658158.1"
                     /translation="GNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDI
                     VWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAIN
                     FSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEG
                     IPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMD
                     FAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            915..2450
                     /gene="gadC"
                     /locus_tag="ACHLAG_005094"
     CDS             915..2450
                     /gene="gadC"
                     /locus_tag="ACHLAG_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFP0658159.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2581..3900
                     /locus_tag="ACHLAG_005095"
     CDS             2581..3900
                     /locus_tag="ACHLAG_005095"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFP0658160.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGIIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
BASE COUNT          965 a         1024 c         1007 g         1042 t
ORIGIN      
        1 ggtaactatg agttcccaca accgctgcac gatgcgctgg ataaattcca ggccgatacc
       61 ggtatcgaca tcgacatgca catcgacgct gccagcggtg gcttcctggc accgttcgtc
      121 gccccggata tcgtctggga cttccgcctg ccgcgtgtga aatcgatcag tgcttcaggc
      181 cataaattcg gtctggctcc gctgggctgc ggctgggtta tctggcgtga cgaagaagcg
      241 ctgccgcagg aactggtgtt caacgttgac tacctgggtg gtcaaattgg tactttcgcc
      301 atcaacttct cccgcccggc gggtcaggta attgcacagt actatgaatt cctgcgcctc
      361 ggtcgtgaag gctacaccaa agtacagaac gcctcttacc aggttgccgc ttatctggcg
      421 gatgaaatcg ccaaactggg gccgtatgag ttcatctgta cgggtcgccc ggacgaaggc
      481 atcccggcgg tttgcttcaa actgaaagat ggtgaagatc cgggatacac cctgtacgac
      541 ctctctgaac gtctgcgtct gcgcggttgg caggttccgg ccttcactct cggcggtgaa
      601 gccaccgaca tcgtggtgat gcgcattatg tgtcgtcgcg gcttcgaaat ggactttgct
      661 gaactgttgc tggaagacta caaagcctcc ctgaaatatc tcagcgatca cccgaaactg
      721 cagggtattg cccagcagaa cagctttaaa catacctgat aacgtttaac ggtaacggtg
      781 tcccgaaacg aacccgtttc gggacaattt ccaaagtctg ttcactggca ttagcaacgg
      841 aaaatattgt tctgaatacg cttcagaaca aaacaggtgc ggttccgaca ggaataccgt
      901 tttaggggga taatatggct acatcagtac agacaggtaa agctaagcag ctcacattac
      961 tcggattctt tgccataacg gcatcgatgg taatggctgt ttatgaatac cctaccttcg
     1021 caacatcggg cttttcatta gtcttcttcc tgctattagg cgggatttta tggtttattc
     1081 ccgtgggact ttgtgctgcg gaaatggcca ccgtcgacgg ctgggaagaa ggtggtgtct
     1141 tcgcctgggt atcaaatact ctggggccga gatggggatt tgcagcgatc tcatttggct
     1201 atctgcaaat cgccattggt tttattccga tgctctattt cgtgttaggg gcactctcct
     1261 acatcctgaa atggccagcg ctgaatgaag accccattac caaaactatt gcagcactca
     1321 tcattctttg ggcgctggca ttaacgcagt ttggtggcac gaaatacacg gcgcgaattg
     1381 ctaaagttgg cttcttcgcc ggtatcctgt tacctgcatt tattttgatc gcattagcgg
     1441 ctatttatct gcactccggt gcccccgttg ctatcgaaat ggattcgaag accttcttcc
     1501 ctgacttctc taaagtgggc acactggttg tatttgttgc cttcattttg agttatatgg
     1561 gcgtagaagc ttccgcaact cacgtcaatg aaatgagcaa ccccgggcgc gactatccac
     1621 tggctatgtt actgctgatg gtggcggcaa tctgcttaag ctctgttggc ggtttatcta
     1681 ttgcgatggt cattccgggt aatgaaatca acctctccgc aggtgtaatg caaaccttta
     1741 ccgttctgat gtcccatgtg gcaccggaaa ttgagtggac ggttcgcgtg atctccgcac
     1801 tgctgttgct gggtgttctg gcggaaatcg cctcctggat tgttggtcct tctcgcggga
     1861 tgtatgtcac agcgcagaaa aacctgctgc cagcggcatt cgctaaaatg aacaaaaatg
     1921 gcgtaccggt aacgctggtc atttcgcagc tggtgattac ttctatcgcg ttgatcatcc
     1981 tcaccaatac cggtggcggt aacaacatgt ccttcctgat cgcactggcg ctgacggtgg
     2041 tgatttatct gtgtgcttat ttcatgctgt ttattggcta cattgtgttg gttcttaaac
     2101 atcctgactt aaaacgcaca tttaatatcc ctggtggtaa aggggtgaaa ttggtcgtgg
     2161 caattgtcgg tctgctgact tcaattatgg cgtttattgt ttccttcctg ccgccggata
     2221 acatccaggg tgattctacc gatatgtatg ttgaattact ggttgttagt ttcctggtgg
     2281 tacttgccct gccctttatt ctctatgctg ttcatgatcg taaaggcaaa gcgaataccg
     2341 gcgtcactct ggagccaatc aacagtcaga acgcaccaaa aggtcacttc ttcctgcacc
     2401 cgcgtgcacg ttcaccacac tatattgtga tgaatgacaa gaaacactaa ccatgtctga
     2461 atggttataa gacaagggag cgataagtca tcgctccctt tttcgtgctt gctgtcgtat
     2521 tgacttaacc gggataagta cgagaatgag cgcacatctg tttaccggaa accagcacat
     2581 atggatatct gctcccgaaa caagaaatta acgattagaa gaccagcgat actagttgca
     2641 ctggcacttt tactgtgtag ttgtaaaagc acgcctccag agtccatggt gacaccacca
     2701 gcaggttcaa agccaccagc cacgacgcaa caatcgtcac aaccgatgcg tggcatctgg
     2761 ctggccacgg tttctcggct cgactggcca ccggtttcct cggttaacat tagtaacccc
     2821 accagccggg cccgtgtaca acaacaggcg atgatcgaca aactggatca tctgcaacgt
     2881 ctcggcataa acacggtctt tttccaggtc aagccggacg gtaccgccct gtggccatcg
     2941 aaaattttgc cgtggtccga tcttatgacc ggtaagattg gtgaaaatcc gggttacgat
     3001 ccgctgcaat ttatgctcga cgaagcccac aagcgtggga tgaaagtaca cgcctggttt
     3061 aacccctatc gcgtatcggt taatacgaag cccggtatta tcagggaatt gaatagcact
     3121 ctgtctcaac aaccggcgag cgtctatgtg caacaccgtg actggatcag aacgtctggc
     3181 gatcgctttg tcctcgaccc gggcatccct gaggttcagg actggatcac atcaatagtc
     3241 gctgaagtgg tttcccgcta tccggtagat ggcgtgcagt ttgacgacta tttctatact
     3301 gaatcaccgg gttcacggct aaatgataac gaaacgtacc gtaaatacgg aggcgcattt
     3361 gcgtcaaaag cagactggcg gcgcaacaat actcagcagt taattgcaaa ggtatcacac
     3421 accattaaaa gcattaagcc gggagtcgaa tttggcgtta gcccggcagg cgtgtggcgt
     3481 aaccgatcac acgatccgct cggttccgat acccgaggcg cggcagccta tgacgaatcc
     3541 tacgctgaca cccgtcgatg ggtggaacaa ggattgctgg attacattgc tccccaaatt
     3601 tactggccgt tctcacggag tgccgcgcgt tatgacgtgt tggcaaaatg gtgggcggat
     3661 gtcgttaaac cgaccaggac ccgcctgtat atcggtatcg ccttctataa agtgggtgaa
     3721 ccttcaaaga tagagccaga ctggatgatt aacggcggcg taccggaact gaaaaagcag
     3781 ctcgatctta acgatgcggt accagaaatt agcggcacca tcttgttccg tgaggactat
     3841 ctgaataaac cgcagactca acaagcggtc agctatctgc aaagtcgttg gggcagttaa
     3901 actcccatgc ttactttgta acaagccggt gctttaccca ccggcgtgtt acaccttgtg
     3961 aaatatccca ctagtaatca tgcttacata agtcaaatta accacgcaat taaccacact
     4021 tagttaaaca gctataaa
//
LOCUS       DAZGVQ010000275         4036 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000275 DAZGVQ010000000
VERSION     DAZGVQ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.278"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..234
                     /locus_tag="ACHLAG_005096"
     CDS             <1..234
                     /locus_tag="ACHLAG_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFP0658161.1"
                     /translation="STGDSYDNAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDW
                     YNNRRLLERLGHTPPAEAEKAYYASIGNNDLAA"
     gene            1596..2573
                     /locus_tag="ACHLAG_005097"
     CDS             1596..2573
                     /locus_tag="ACHLAG_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP0658162.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(2852..3592)
                     /locus_tag="ACHLAG_005098"
     CDS             complement(2852..3592)
                     /locus_tag="ACHLAG_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP0658163.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(3713..3847)
                     /locus_tag="ACHLAG_005099"
                     /pseudo
     CDS             complement(3713..3847)
                     /locus_tag="ACHLAG_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
BASE COUNT         1062 a          959 c         1012 g         1003 t
ORIGIN      
        1 agtacaggcg actcgtatga caacgcgatg gcggagagca tcaatggtct ttacaaagcg
       61 gaggtaatac accgtaagag ctggaaaaac cgtgcagaag tggaactggc cacactaacg
      121 tgggtggact ggtataacaa tcgacgattg ctggaaaggc tgggccatac tcctccggca
      181 gaagcagaaa aagcttatta tgcttccatc ggaaataatg atctggcagc ctgagttcac
      241 agataaaaca ctctccagga aacccggggc ggttcagcat gaccggattt ttatcagtgg
      301 gtagacagtt gggagtgaag cctgaacaca cccattgttg tctgtgcaca tcaagcgtgg
      361 ccagaatacg acccgacgcg ctgtaaattg caaactgatg tgtatatacc gaaccggtat
      421 atcctggcgg tacagggttt atttttaacc ataggaattt atgaccacaa cgtgtcagat
      481 aattccagac atgaactatt gatgcgcgca tttagttcct tagtaaaaag tcaccaatgg
      541 taattatgat gtgcagcata atcagctgca catacctcta catctgtcac tctaatgact
      601 ttctcctacc gcaatcatca ctccactgat aataatcaga catatagaca cattattaaa
      661 ttaacgaatg gccgttccgg tgagatccga ttttctgtct tataacaact ttccggaagt
      721 gtaacacggt tgcgtcttgg ctcttgcacc cttttctgtc gttgtgaacg ggcccacagg
      781 gaatgaagga gacacaatat atagtgtcat tagcatctaa attaagcact atatgttgtg
      841 ctgacagaag gtggcgttgt tgtgggggaa aaggaagatc cgtaactatc aggactctga
      901 cgattagtct taagactgtc ggtaaattaa gtgatatcag ttgtcaggat attcatggag
      961 tcagtctgta tgcacctttg ccattgaaac aaacatatga agtgtacttc ggatatgacc
     1021 gtatgttcag gaagtacggc agattgagtc gaaaattacc tgtaaagaca gacttcagaa
     1081 aaaacggtgc tcgccgcaga ccggtgaccg ggcgaagccc gcgaaccggc gaggaagcgc
     1141 aatggatctg taatcacagt gagcaggtac gccctgaagc cttcataatc ggtagtgtgt
     1201 gttgtttttc cggaatgatt tatccacata tccggaggct cgatccagta aacagatcca
     1261 tgaatgatca acaaaggatc cattaaagat ccccatatcg ctgcaaacct tgtcattaat
     1321 ggtccgggag cacgatcaca taagctgtgg catgttactg ataaactgta acaagttaat
     1381 gataagctgt attaagtaat acatatactg cagtaagtta atgacataaa ctatggtcag
     1441 tacgccagac tcagctgtta aatacaggct gcagattttt cttcagtcag ttagcggggt
     1501 tctgacacac gattttctgt ttattctttt actgtccaca ggcaggaggc tttctggaaa
     1561 acgaaaattc agacatcaaa aaactgttcg gcgaggtgga taagtcgtcc ggtgagctgg
     1621 tgacactgac accaaacaat aacaacaccg tacaacctgt ggcgctgatg cgtctgggcg
     1681 tttttgtacc gacccttaaa tcactgaaga acagtaaaaa aaatacactg tcacgtactg
     1741 atgccacgga agagctgaca cgtctttccc tggcccgtgc tgagggattc gataaggttg
     1801 agatcaccgg cccccgcctg gatatggata acgatttcaa gacctgggtg gggatcattc
     1861 attcctttgc ccgccataac gtaattggtg acaaagttga actgcctttt gtcgagtttg
     1921 caaaactgtg tggtatacct tcaagccagt catcccgcag gctgcgtgag cgcatcagcc
     1981 cttccctgaa gcgcattgcc ggtaccgtga tttcgttttc ccgtaccgat gagaagcaca
     2041 cccgggaata catcacccat ctggtacagt cagcctacta cgatactgaa cgggatattg
     2101 ttcagttaca ggctgatccc cgcctttttg aactgtacca gtttgacaga aaggtccttc
     2161 ttcagcttaa ggcgattaat gccctgaagc gacgggagtc cgcccaggca ctttacacct
     2221 ttatagagag cctgccccgg gatccggcac cgatatcgct ggcgcggctg cgtgcacgcc
     2281 tcaatctgaa gtctcctgta ttttcccaga accagacggt cagacgggca atggagcagc
     2341 tgcgcgagat tggatatctt gattacacgg agatccagcg ggggcggaca aaactcttct
     2401 gcattcacta ccggcgtccc cggttaaaag caccgaatga tgagagtaag gaaaatccgt
     2461 tgccaccttc acctgcggaa aaagtcagtc cggagatggc ggagaagctt gccctgcttg
     2521 aaaaactggg catcacgctg gatgacctgg aaaaactctt caaatcccgc tgaacataaa
     2581 ctgtagtcag tgaagagtgc tcctttactg actacagctt atattatcag gtgcagtgag
     2641 tggtctgctc actgcagttt atattcagtt tcatgcagtg ctgcctgtgg ctgagctgcc
     2701 atctgcctgt cccttacgtg agtcaccccg taagctgatg ctgaggcatt gctcccttca
     2761 taaaacatgg tttactcact acagcttatc tacctgctcc agcttatgtt atgtctgttc
     2821 tgctgaccac tgcttatata aggaagtgcc gttacttcag gggaggcagt gtacgcagga
     2881 tctccgcagc atcccgcccg tcacctgtga aaggcactgc cagcgtggca gccatatcca
     2941 gtgcaaacac tctggtataa acctccatag aacgtggatc cctgtgacca gccagtgcct
     3001 ggatgacttt ccggggctgg cggtgataga gcatgtgcat gatatagctg tgccggaagg
     3061 tgtgtggtgt gaccggaatc gaaaagtgta ctccgtcagc ttcggcccgt ctgacagcct
     3121 gcttcagcca gttgcgcatg gtttcgtcgg tcacggccca taatggttca cgacgacggg
     3181 gccgggtggt gatcatccag ctttccatct gcctgacata gcttatatcc gtcagcggaa
     3241 ccaggcggac ttcatctttt ggcgggcgtc cgcgtcgcgc acgcactttt tcggacagga
     3301 tccgcacaaa cggtcttact ccatccaggt caaatgattc cggtgtcagc atccgggctt
     3361 cgccaatacg cattccggta ttccagaggg tggcgaacag catatggtga cgctgatccg
     3421 gcatataaaa aagaagggca ctcacttccg gggccagcag gtaggccggt gtggcccctg
     3481 tggaagtggc cattcttctc agggagagcg ctgttgcaaa atccaccccc ggcgcaatgg
     3541 gtaacaggga gacgcgttct ggtgaattct gcaggggaat gacattgttc atgagttatt
     3601 tttcacgtag cacacgcact gtttatggat acaggtattc tgacctgaac gcccggatcc
     3661 ttccacgaca ataaatcgca gcgcgcggat ccttttattt gcttatcacg cattattgcg
     3721 ggtaaaacca aatacgtaaa ggtacgtttt gcagcaggga tcccgtatta agccagtcgt
     3781 ggcgcgggct gagggctggt ttaccgtttt tgggagaatt ggggtaatgc caggatgaat
     3841 gggatcggag aaaacagaga aagggaacag aaagggagaa ggtgcaccac gtgtattctg
     3901 gcccgggacg tcaccgtccc cctgacagtt tgactttaca caggaaggtt gcagactcag
     3961 cataatgact gatttatatg caatcctgtc catgattaat ataaatcata ataatgatta
     4021 tgcagaaaat cacttt
//
LOCUS       DAZGVQ010000276         4022 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000276 DAZGVQ010000000
VERSION     DAZGVQ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.279"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..137)
                     /locus_tag="ACHLAG_005100"
     CDS             complement(<1..137)
                     /locus_tag="ACHLAG_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP0658164.1"
                     /translation="MTALLTLEEIKAHLRVEHDADDDMLMDKVRQATAVLLAYIQGSR
                     D"
     gene            complement(134..2556)
                     /locus_tag="ACHLAG_005101"
                     /pseudo
     CDS             complement(134..2556)
                     /locus_tag="ACHLAG_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(2502..2723)
                     /locus_tag="ACHLAG_005102"
     CDS             complement(2502..2723)
                     /locus_tag="ACHLAG_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658165.1"
                     /translation="MAKIRYLQGTHDARPGDIRDVAQPCAEVLVRLGKAEYITARRPA
                     GQKKKHDAEHGECGTFCDEPEKIRNQDVM"
     gene            complement(2768..>4022)
                     /locus_tag="ACHLAG_005103"
     CDS             complement(2768..>4022)
                     /locus_tag="ACHLAG_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP0658166.1"
                     /translation="VEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNG
                     NVAAVASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVL
                     KSAVGAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRV
                     HAQVSGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVR
                     NALAEAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVAA
                     NLQPTGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGAFQGLPVIVSQYVGDQLVL
                     VNAPDIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERW
                     INWRRRRTAAVAVITGVNYGSASGG"
BASE COUNT          820 a         1322 c          987 g          893 t
ORIGIN      
        1 ttatcccggc tgccctgaat gtaggccagc agcacggcgg tagcctgacg aaccttgtcc
       61 atcagcatgt catcatcagc gtcatgctcg acacgcagat gtgccttgat ctcttccagt
      121 gtcagtaatg ccgtcatttt ccgcctccag catcccgccc acgttttgca gccagggtcc
      181 agcctgatga atgagcttct ccgggtttat caccggtcat actgttgcag tgccacagcg
      241 agccgcccca cgtcaccgta tcgccggggt ggtaggtttc accggctctg aacacaccgc
      301 ggtagagcat caccggcagg gaaaatgttt tttccgtacg ctggccactg ctctgccgga
      361 ccaccacaga gaacaaccgt tcacccgtca tgctgacgtc aatatcagcc accccgtcaa
      421 ccaggcattc ccatccccgc atcccgtgcg ttttttcata cgcccgccag agtccgccct
      481 ggtgtgtggc atacgtgccc cggggaaagg atttttgatc gtcaatggcg gggagtattt
      541 ccagtgccgt gccatcacgc ccgtcctgcg gagccggcag ggcactcacc gcatccagaa
      601 ccgccttctg cagaacatcg ggatcatagt cacgaccatc acgcggaaca ggaatatggc
      661 ttaccgcctc cttcaccatc tgttcaagca tcggacgcac atcatccggg gtgatacttt
      721 taccgtccgc cggctgcgga atatttgcga ccgcatcatt caccgccttc tgcagtactt
      781 ccggatcgta atcacgacca tcacgcggaa caggaatatg gctcactgcc tctgtcacca
      841 tctgttcaag catcggacgc acatcatccg gggtgatact tttaccgtcc tccggctgcg
      901 gaatatttgc gaccgcatca ttcaccgcct tctgcagtac ttccggatcg taatcacgac
      961 catcacgcgg taccgggatg gcccccacag cgtcatccac catcgcctgc agaaccggac
     1021 gcacctcatc caccgtcaca tgcttctgta ataccacaga cagggaagcc agtttctctt
     1081 caaacgcttg tgcctgcgcg gctattttcc cctcaaatgt gcgctgtaaa tccgccagca
     1141 ccgcagcgaa ttcttcgccc agcgcacgga taatggacag ttcacgttca ttcatttttt
     1201 cagaatcccc ctgaacatcg ccttcaccgc gtcatgctct gtttcactga ttgccttatt
     1261 accgtcagat gcgccgtcag gcggttgcgc tgaggctgtt ttcccggtcg acgcaaacgg
     1321 atcctcacgg gcatcacgac gggacagcgc ctccagactg tagttctgct gctgaagata
     1381 cagtgcatca ccgccggcaa ggggcggcag gttctcacgt ttacgggctt cattgggcgt
     1441 gagaagcgta tttttcaccg attcacccag tgttttcatg cgccgttcgc tgtccattct
     1501 cagcagtgtg gtgacatcaa actccgtgct ctcgttttcc cccgtttcca gcgcctcatc
     1561 cagtaacagc tcaatggact caatcagcgt ctgcagacac tgggaataat actgctgctc
     1621 cagcgcctcc acgttatcac tggagggagg ttgtcccacg ccaatcttgt aggccggaac
     1681 acggaacacc gaacagacaa tttcagccgt cattttcagt tgttccaccg tctgcgcatc
     1741 caccggtgaa aacgtcgtgg ggctgtattt tgccccgttg ctcagtatgg ccgtcttccc
     1801 ggcattttcg cctgtatacc cgctgtccca gttgctcttc agtattttcg cattttcttc
     1861 cgtaatactg ccggggatct caatcacccc ggacggcctg ccgccatttc tgaaaaaata
     1921 cgtcgaattt tcctgaatat gatgcccctg cgtggcggcc agcccggcgg catacaccgg
     1981 cggcaaccct ataagcggat gaaaaaaaca gttaaaccgg tcgtggatca cttcccgggc
     2041 aggcaccgtc accgcctcag tgatcccaca gttccggtcc ggcgtgatac ggtagaacac
     2101 ctcgccgtca tccgccacca gaggttcaac ccggttccag tccagaatac gcagttcttt
     2161 tatctgcccc cgggagttac ggattttcag caccaccgta ttaccgtgac gcagttttga
     2221 gttcagccac agttcaaaaa actggatacg attctgctgt gcattgggac gacgacagag
     2281 acgggcaata tccccctgcc gtttttcacg gcgtatcccc tgtgtatccg tctgcatcag
     2341 gcgcagccgc attttggcga tatcctggga tatcagcgaa atacatgaaa acaccgcatg
     2401 aaaggagagg acactttccg gatcggcttt cacgccctgc tgccaggcac cggcaaaggg
     2461 ctcagccacc gcctgaaaca ggctggtcca gcccacctct tttacatcac gtcctgattt
     2521 ctgatttttt cgggttcgtc gcaaaaggtt ccacattcgc catgctccgc atcatgtttc
     2581 tttttctgac ccgccggacg tcgagctgtt atgtactccg ccttccccag gcgaaccagc
     2641 acctccgcac acggctgtgc gacatcccgg atatccccgg gccgggcatc atgtgtaccc
     2701 tgcagatatc ggatttttgc catcagttac tgcgggaagc tctcacctcc cgccctcctc
     2761 atcagactca gccgccggac gcactgccgt agttcacacc ggtgatcacc gccaccgccg
     2821 cggtacggcg acgacgccag ttgatccagc gctccgcacg gatggccaca ctgcctgtct
     2881 ggaacatgga aaccagctcc accggcgacg gcgtggtact gtcgccggtc ggctcagact
     2941 gcatttccag tgatgcctcg cgggacatat ccaccgccac gccgccgtca tccgccagat
     3001 aaatatccgg ggcattcacc agcaccagct ggtcacccac gtactgggag acaatcaccg
     3061 gcagcccctg gaaggccccc ccaagcaggg tcatatccgg gtattctttc tgacccagcg
     3121 catttttacg cattgacaac gccagggcat tggtgctgga catcagccag accgcaccgg
     3181 tgggctgcag gtttgctgcc acaaactgac caaacgcggc ctccgcatcc gcatccgggt
     3241 taccggttga tgccgtgccc ttcacatcat gggtgatgga cgccggggag acatctgcca
     3301 ctgcggcttt tttcggatcc acaaagtctg tatccagacg cgccaccacc gcttccgcca
     3361 gcgcattacg gaccagtgca tcagcagccg gactggaaaa acggatcagc tcttccgtca
     3421 gtactgcaat ggccgacacc ttcgcatgac tgaaggtgat ggactcaaaa tcaaacttcg
     3481 tcaggggttt tgccttaccc tcccccaccc agccagcagc accgccggac acctgggcgt
     3541 gcacacggat attgaacggc acctgacgaa gtgcagggat cccaccctga ccaaatcgcc
     3601 cgataatggt ctgaggacgc aggtaatcaa taaagtcctg cgcatattcc tgatattcag
     3661 acaggctgcc tgcccactgc ggatccgtgg tggtccccgc gcccactgcc gatttcagga
     3721 catgatgcag acgactgtca tccggatact gacgacgggc cacttccagg gcttcagagc
     3781 ggacaccttt agccgcagcc agtgatttgg caaagcgggc gaagccaatc cccttatcca
     3841 gtttctgctc cacacggatc accggcgctg aagccaccgc ggccacattc ccgttaccgg
     3901 cctgtttcac cggctgcgcc gtggcggcct taccggcttc cagttcacgc aggcgcttca
     3961 ggtgcgcatc cacctgacgg atttccgctg cggtgttgtc gtaatgctct tcctcctcca
     4021 ca
//
LOCUS       DAZGVQ010000277         3943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000277 DAZGVQ010000000
VERSION     DAZGVQ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.280"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(41..364)
                     /locus_tag="ACHLAG_005104"
     CDS             complement(41..364)
                     /locus_tag="ACHLAG_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658167.1"
                     /translation="MSNYELQIFVDNDTAMMVQSFIDAGVEIDFDRLLKLMAENSEAI
                     EDFIQGIETGEPRFMFPVTDSNMKRLIVEETNRYSVSPEKYLKAAIAILYADNILVTD
                     SRVVH"
     gene            complement(739..969)
                     /locus_tag="ACHLAG_005105"
     CDS             complement(739..969)
                     /locus_tag="ACHLAG_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658168.1"
                     /translation="MLPRYKFYKLASHLIRQDSRSKEYEDVTLDRNKKRLKPHQIEQL
                     RYELKSLDIELDYSDPTQFKRVYELIKNWGKE"
     gene            complement(969..1256)
                     /locus_tag="ACHLAG_005106"
     CDS             complement(969..1256)
                     /locus_tag="ACHLAG_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658169.1"
                     /translation="MKNRHTLTRITKTAFEELNRIKNITGLPHSAVMKLAINRDINEQ
                     DLLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLADKLEI"
     gene            complement(1258..1813)
                     /locus_tag="ACHLAG_005107"
                     /pseudo
     CDS             complement(1258..1813)
                     /locus_tag="ACHLAG_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999794.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2161..2349
                     /locus_tag="ACHLAG_005108"
     CDS             2161..2349
                     /locus_tag="ACHLAG_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658170.1"
                     /translation="MVNLPPELGSPDVWTGGGVGLIAGLLLRYFIVRVFVVFKLFGRE
                     WSFDLKDRTRTDNHTEPR"
BASE COUNT         1121 a          804 c          714 g         1304 t
ORIGIN      
        1 atcataccaa tcaatacaaa acagataatc accaattcca ttaatgcacc accctcgaat
       61 ccgtcaccaa aatattgtca gcgtacaaaa ttgctatagc cgccttgaga tatttttcag
      121 ggctaacgct gtatcggtta gtttcctcaa caataaggcg cttcatgttt gagtcggtaa
      181 cgggaaacat aaaacgtggt tcaccagttt caattccctg aataaagtct tcaattgctt
      241 ctgagttttc agccattagt tttaacagcc tgtcaaaatc aatttccaca cctgcatcaa
      301 tgaagctttg caccatcata gcggtgtcgt tatcaacgaa aatctgtaat tcgtaattgc
      361 tcatttttaa aatctccttt ttatcagacc tcctcaatcg ctctgtaacg agctaaatag
      421 tctttttcag gggtagcctg taggggtgta ctacataccc taaaataaga tagttttttg
      481 cgttagaatg cgattgagag cgtctgagag tgggtaaaac cgttttttgc ctttagtctg
      541 agactattaa ccccatctca ctatgaaatt gactaaacca gctttttgtt actgaggttt
      601 ttagtgtgaa agatggggat tgcataatca gatagatgcc atcattatca atgtcgcccc
      661 atccactatg tacatcaatt agatttcgaa aatatttaaa ggctttgtct gttttaagtt
      721 tgagggtgaa tactttcttc attctttccc ccaattctta atgagttcat agacccgttt
      781 aaattgagtc gggtcactat aatcaagctc gatatccagt gactttaatt catagcgtaa
      841 ctgctcaatt tgatgtggct ttagtctttt cttgttacgg tcaagcgtaa catcttcata
      901 ttcttttgag cggctatctt gtctgattag atgtgatgcc agtttataaa atttgtatct
      961 aggtagcatt aaatctccag cttatccgct aacttgtgaa tcttgatact tagtaacctt
     1021 gctaaatcca gtccaaaagc atttaaagat ttcagactat caaggtcatc ttgcttaatc
     1081 ctgatatggt gtcttgcagc ctctaagcga ctattaagca aatcctgttc gttaatatca
     1141 cggttaattg caagcttcat cactgctgag tgtggcagac ctgtaatgtt tttgattcgg
     1201 ttaagttctt caaaggctgt ctttgtaata cgggtcaaag tgtgtctgtt tttcatttta
     1261 aaattcctct gcttgtgcgt atacgtcaaa ttcaaagtct ggttcactgg ttgtaaggct
     1321 gatatttccc atttccgcat gatgattgat tgcctcaata actttcatgc aaatagtggc
     1381 taagttagcg caatatttat cacttggtag ctcatggctt aatcgggttg cttgacgctg
     1441 tagataactt gttattgtgc ttgagctaat cttgtcaata tttcgtagta aatagaaaac
     1501 ttttcggtga taattatctc ttccggcatt acggcaagtt ccgataataa tatcttcaat
     1561 tcgtgatggt aattgttcat agctgtcaaa actcatcaag aaagttttta aagtattttc
     1621 tttttctttg cgtactgaca taaatctgtc tcgtggcgta atgctacgaa tgaccgcttc
     1681 aaggttctta cgttcggatt tgctaaaggt tggtacttcg tagctggtgg ggggtgtctg
     1741 tgtagctgta accgttgctt ctgtttcttg ctttgctctc ttcaagaaat ctttgaaatt
     1801 ctctgtcgtt cattcatatt ctgaccctca tgaagtgtta ttattctgtt aactatatgt
     1861 acaatgctag cattttcaaa gactaatgtc aagcattttt taacatttca ttaacatctg
     1921 tggtataatg ggtttatggg gagagtaaaa tataaaggcg tgactatgag gtttattcga
     1981 taggggttgg tacaagccca actaaccgaa tcaactatgt atttggtcgc atgttttgca
     2041 ttgcacacag acctcttcaa tcgccctgta acgagctaaa cagcctcttc aggtcaactc
     2101 ataaggtcag gtactttttt gtgtcagaat gcgattgaga gcgtcttaca aggaaaacta
     2161 atggtaaatc tccctcctga actggggtca cctgatgtat ggactggtgg aggtgttggg
     2221 ctgattgcag gcctgctact tcgatatttc atcgtcagag tgtttgtcgt gttcaaattg
     2281 ttcggtcgtg aatggtcgtt tgacctcaag gacaggacca gaacagacaa ccacacagaa
     2341 ccccgctaaa ctcccttcaa aacccctata ccatctcgta caggggtatc tttagcattt
     2401 tgtgctatct attaaaaaac agcttgacag aaccggaaag ctgtatctat caagtgttca
     2461 cctctctgac agtaatttct aaaagttctt atatttcaat aagttgtaat tagccatata
     2521 tagagaactg acaagtctct tcacagttaa ctctgtaacc ttaacagtaa cttctaaaaa
     2581 cacttatttt tcaataagtt gtaatcaccc aatataatag ctgttcagct tcttcacaga
     2641 gaaacttcag aggtcttctt aatcaatcca ctaagcgaga gaagagcgat aaccccagag
     2701 agggaagctc ctttcttaac cgacctctta aacgaccttt acagaccttg ttcaaggtaa
     2761 tcacaataga tttttcttcc ctcggtttcc cgtgttcctc ggtttgtcta ttgagggctt
     2821 tgaaatgtcc ctgtaacttc cctcttagac agggaagaat aatattggtg atacaagcga
     2881 agcgcccctc taaaaagatt ttttagctat tttgtttaaa aacatatcaa acgatacatt
     2941 ttattgattt aggtcaaaga caaaagcatt gacaagatgg taagattgtt cacatagccc
     3001 tctcttaaca gactttttaa gagcttttga gaagtttgtt aaacagacac tctttctgga
     3061 gtgttgtctt ttacttaaga ggcttgttta ggtaactctc aagacatttc ttaagacttc
     3121 actgttaaga cagaacctac cttcggtttc atggttctct tcctcccgtt aaacacccct
     3181 tcctagcaac ttcatactta tctgtcaagt acctttacag actctatcaa aaccctctaa
     3241 atctgacctg atatagatgc tgtgaaggca tgttctctag gtctgtgtag cttacttagt
     3301 gggaagagag ggtgatacat gagaaagaac cctcctgtta aaaagatatc tcttcctccc
     3361 gttaaacgct cttatcaagc cctctgcata atcactacca aaaaccttac aaggtaatat
     3421 ccaggcctct tatagttgac ctgatacaga agttttacag acatactctc tagatactat
     3481 caagggacac taagcgagag agagggtgat acatgagaaa gaaccctcct gttaaaaaga
     3541 ccacttagaa gtctgagact ttagtaaaat ctgtgaatat aacttctcag acgctctgta
     3601 acgtattcta agcgatttta cccctttatt ttaaggtggg tggtacatcc ctacagatta
     3661 cccctaaaaa tgtggttaaa actcgctaca gagctttcta tgagattagc ttcatgaagt
     3721 aattcattac tgaatcacct aatcgaatca actatgaagc ttaataggat tgtgcaaaat
     3781 ctgtacagta gttatttagc ttctgaaaat ttttataaaa atcgtagtac cccctctgca
     3841 ttcccccctt caccgataat cttaaaaaat atctacactc aattgattcg gtagaatcta
     3901 cctcatgatt gatggctgtt aagtacccca gattcacgat ata
//
LOCUS       DAZGVQ010000278         3943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000278 DAZGVQ010000000
VERSION     DAZGVQ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.281"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(61..273)
                     /locus_tag="ACHLAG_005109"
                     /pseudo
     CDS             complement(61..273)
                     /locus_tag="ACHLAG_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            423..1091
                     /locus_tag="ACHLAG_005110"
     CDS             423..1091
                     /locus_tag="ACHLAG_005110"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP0658171.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            complement(1036..1173)
                     /locus_tag="ACHLAG_005111"
     CDS             complement(1036..1173)
                     /locus_tag="ACHLAG_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleocapsid protein"
                     /protein_id="HFP0658172.1"
                     /translation="MAYEPCQGVIIGIMPQHVLSKNVLRLDAIFSLKRKTLLQYLEPW
                     F"
     gene            complement(1146..1730)
                     /locus_tag="ACHLAG_005112"
     CDS             complement(1146..1730)
                     /locus_tag="ACHLAG_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0658173.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(1730..>3943)
                     /locus_tag="ACHLAG_005113"
     CDS             complement(1730..>3943)
                     /locus_tag="ACHLAG_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFP0658174.1"
                     /translation="ASSAASSASSASASKDEATRQASAAKGSATTASTKATEAAGSAT
                     AAAQSKSTAESAATRAETAAKRAEDIASAVALEDASTTKKGIVQLSSATNSTSETLAA
                     TPKAVKAAYDNAEKRLQKDQNGADIPDKGRFLSNINVYSKGEVDQKKGMRYVVVNAPA
                     GVQEGKYYPLVIKRNDSHRASRVVISTPSRTANHRMNNCEFNGFVCAGGWTDRSSYAC
                     GMFWAYSSSERAIHSILMSNKGDTVDSVFYIEGGAFPVEAFLEEGLSVTAPASDYVVA
                     ETTYKFGATDPYSESVAVNLILDFKQGNGFYSSYPVLSKSDISGNKIYANDEVIVRSQ
                     NALRMIAGDYGVIWRNDGANTYLLMTDKGDQYGGWNGLRPFAVNNATGEVTINTPLNS
                     PKGIKGNSDTATKLQTAIKISGVSFDGSTDITLTAAHVAAFARRATDTYADADGGVPW
                     NAESGAYNVTRSGDSYILVNFYTGVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDWA
                     EVYTSKNLPPESYPVGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDM
                     RGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAH
                     THSVSGSTNSAGAHTHSLANVNTASANSGAGSASTRLSVVHNQNYATSSAGAHTHSLS
                     GTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAENTVKNIAFNYIVRLA"
BASE COUNT          995 a          991 c          857 g         1100 t
ORIGIN      
        1 gatattactt taatcaaaag ttgacgtgcc agattcgaag taaatttata aaatatcaaa
       61 tcaatataca ttttgcacct tcgagatagt aatgactgtt actgtaacat tagttacaaa
      121 cagcttcata cccagagcta ctgatgtatc aactcgattc aacaataccc gatatttatt
      181 ccatgcctcc agcaacgatc tttcttcctc cgttgcgatt tccagatcta cagcatcctg
      241 cagtggcgca atatactcac tgaattcctg gatgtagaac tgtgtggtga cggtcttcca
      301 gccattcggc tcctgctgta tcgaagcata ccaggctatt tcaatatcgc tatgctgcgg
      361 cagcatttaa ccccttgtaa ttcatcgcca taattgattt aattcacaaa taaaactata
      421 acatggtgaa attaatgaaa aaaaacacag atgatggggc taaaatttac acaccactta
      481 ccctaaagct ttatgactgg tgggttttgg gagtatcaaa tcggcttgca tggggatgtc
      541 ctacaaagga acaccttctt ccacactttc tggaacattt aggtaacaac catctggata
      601 ttggtgttgg aactgggttt taccttactc acgtacctga gagtagtctg atatctttaa
      661 tggatttgaa cgaagctagc ctgaacgcgg catctacaag ggctggggaa tcaaaaatta
      721 aacataaaat taaccatgat gtttttgaac cttatcccgc ggcgttacat ggtcaatttg
      781 attccatttc catgttttac cttcttcact gcctgcctgg aaatatatct acaaaaagct
      841 gtgtaatacg caatgcggcg caggccttaa ctgacgatgg aactctatac ggagccacaa
      901 ttcttggcga tggagttgtg cacaatagct tcggtcaaaa actgatgcgc atttacaatc
      961 agaaaggcat cttttcaaac acaaaagatt ccgaagaagg cttaacacat atactctcag
     1021 agcatttcga gaatgttaaa accaaggttc aaggtactgt agtaatgttt tccgcttcag
     1081 ggaaaaaata gcatccaacc gcagcacgtt cttgcttaag acgtgctgcg gcataatccc
     1141 aatgattact ccctgacagg gttcgtaggc cactcaatat caggtgcagt tgatgtatca
     1201 acacggttca gcaacacccg atactttttc caggcttcca gcaatgaggt ttcttcctcc
     1261 gttgcgattt ccagatccac agcatcctga agtggcgcaa tatgctcact ggctacctgc
     1321 atcaggctgt tttttgtttc ttccgcctct cggatccgga acagtttttc tgcttctgca
     1381 tctttcaccc aggctgtgcc gttccacttc tgaaactccc cttccggcga taaccaggta
     1441 acattttccg gtaacggacc gagttcagaa ataaataacg cgtcccctga cgctacgtca
     1501 taaaccgttt taccccgatg gtcttcaacg agatgccacg atgcctcatc actgttgaaa
     1561 acagccacga agcctgccgg aatatctggc ggtgcaatat cggtactgtt tgctggcaga
     1621 cctgtatgag gcggaatata tgcatcacct tcaccaataa attcattagt tccggccagc
     1681 agattataaa tttttatggt ccgtggttgt tcactcattc tgaatgccat tatgcaagcc
     1741 tcacaatgta gttaaatgcg atgtttttga cggtgttttc cgcgttacca gcagcgttaa
     1801 cggtgatggt gtgtccatgt gagccaatcg caaccgagtg cgtatgagca ccaataccga
     1861 cagtatgtgc gtgtgcacct gcgcttgcag cagtgccgga cagtgagtgg gtatgtgcgc
     1921 cagcagatga tgttgcatag ttttgattat gcacaacaga caatcttgtc gatgcactac
     1981 cagcaccgga gttagcacta gccgtgttca cgttggctag tgagtgtgtg tgtgctccag
     2041 ccgagtttgt agagccgctc acactgtgtg tatgtgcccc ggtgttattc gtggatttag
     2101 tgccgtaatc aaacgacgat gtggttttcg tccccaaatc cgtactggat gcgctggcgc
     2161 tgtgggtgtg cgatttaatg ccgtcctgtt cctgagataa tacggcccga ccactggcag
     2221 gtttgccctt aatcgtccag ccacgcatat cagggatcac gcctgacgga taagcggctg
     2281 caagtttcgg gtaagcagat ttgtcaaaag tctgcccctg catcagggca tagccagacg
     2341 gaacggtatc tgatggccac gggattggtg cgccgactgg gtagctttct ggcggaagat
     2401 ttttcgaggt ataaacttct gcccagtctt cctcaaaacc ataaccgtct cttgaagaac
     2461 ggtagaacag accaccattt ctgtaatgcg ccttcatctg caaagtccgg caacttccga
     2521 ctccggtata gaagttaacc agaatatagc tgtcgccaga gcgggtgaca ttgtaagcgc
     2581 ctgattcggc attccaggga acgcccccat ccgcatcggc atacgtatcc gttgctcttc
     2641 tggcaaaagc agcaacatgc gcagcggtta aagtgatatc agtagaacca tcaaacgaaa
     2701 caccagatat ttttattgct gtctgaagtt tcgtagctgt atcagaattt cccttaatcc
     2761 ccttaggcga attaagtggt gtattgatcg tcacttcacc tgttgcgtta tttacagcaa
     2821 atggtcgaag accgttccag ccaccgtact ggtcgccttt atcagtcatg agcaagtaag
     2881 tgtttgctcc atcatttcgc catatgacac cgtaatcgcc tgcaatcatc cttaacgcgt
     2941 tctgactacg gacaataact tcatcattag cgtaaatctt attcccgcta atatctgact
     3001 tactcaaaac gggatacgaa ctataaaatc cattaccttg tttaaaatct aaaattaaat
     3061 ttacagcaac tgattcagaa taaggatcag ttgcgccaaa tttataagtt gtttcagcaa
     3121 caacataatc agaagcaggt gcagtaacgg agagtccttc ttctaaaaaa gcctctacgg
     3181 gaaatgcccc accttctata tagaatacgc tgtctacggt atcgccttta ttactcatca
     3241 gaatggaatg aatagcgcgt tccgatgatg aataagccca gaacatacca caggcatagc
     3301 tactccggtc cgtccagccg cctgcgcaaa caaaaccatt aaactcacag ttattcattc
     3361 tgtgattagc tgtgcgactt ggcgttgaaa tgacaacgcg ggatgcccga tggctgtcat
     3421 tcctttttat cactaacgga taatatttac cttcctggac acccgccggg gcatttacca
     3481 caacgtatcg catccccttt ttctgatcca cttcaccttt gctgtaaaca ttgatgttac
     3541 tcaggaagcg gcccttgtcg ggaatatcag caccgttctg gtctttctgc agacgtttct
     3601 ctgcattgtc ataggctgct tttactgcct ttggcgttgc cgccagcgtt tcagacgtgc
     3661 tgttggtcgc actgctgagc tgtactatcc cctttttcgt cgtgctcgca tcctcaagcg
     3721 ccacggcgga tgcaatatcc tctgcccgtt ttgctgctgt ctcggcgcgc gttgccgcgg
     3781 attccgccgt acttttgctc tgagctgccg ccgtcgcact gccagctgcc tctgtcgcct
     3841 tcgtggatgc cgtcgtggcg ctgcccttcg ctgctgacgc ctgtctggtc gcctcatctt
     3901 ttgaagcaga cgccgatgat gccgatgacg ccgccgaact ggc
//
LOCUS       DAZGVQ010000279         3928 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000279 DAZGVQ010000000
VERSION     DAZGVQ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.282"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(40..2462)
                     /locus_tag="ACHLAG_005114"
                     /pseudo
     CDS             complement(40..2462)
                     /locus_tag="ACHLAG_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(2408..2629)
                     /locus_tag="ACHLAG_005115"
     CDS             complement(2408..2629)
                     /locus_tag="ACHLAG_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658175.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(2674..>3928)
                     /locus_tag="ACHLAG_005116"
     CDS             complement(2674..>3928)
                     /locus_tag="ACHLAG_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP0658176.1"
                     /translation="VEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNG
                     NVAAVASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVL
                     KSAVGAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRV
                     HAQVSGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELICFSSPAADALVR
                     NALAEAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASSGNPDADAEAAFGQFVAA
                     NLQPTGAVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVL
                     VNAPDIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERW
                     INWRRRRTAAVAVITGVNYGSASGG"
BASE COUNT          788 a         1283 c          984 g          873 t
ORIGIN      
        1 gcagatgtgc cttgatctct tccagtgtca gtaatgccgt cattttccgc ctccagcatc
       61 ccgcccacgt tttgcagcca gggtccagcc tgatgaatga gcttctccgg gtttatcacc
      121 ggtcatactg ttgctgtgcc acagcgagcc gccccacgtc accgtatcgc cggggtggta
      181 ggtttcaccg gctctgaaca caccgcggta gagcatcacc ggcagggaaa atgttttttc
      241 cgtacgctgg ccactgctct gccggaccac cacagagaac aaccgttcac ccgtcatgct
      301 gacgtcaata tccgccaccc cgtcaaccag acattcccat ccccgcatcc cgtgcgtttt
      361 ttcatacgcc cgccagagtc cgcccaggtg tgtggcatac gtgccccggg gaaaggattt
      421 ttgatcgtca atggcgggga gtatttccag tgccgtggca tcacgcccgt cctgcggagc
      481 cggcagggca ctcaccgcat ccagaacagc cttctgcaga acatcgggat catagtcacg
      541 accatcacgc ggaacaggaa tatggcttac cgcctccttc accatctgtt caagcatcgg
      601 acgcacatca tccggggtga gacttttacc gtctgccggc tgcggaatat ttgcgaccgc
      661 atcattcacc gccttctgca gtacttccgg atcgtagtca cgaccgtcac gcggaacagg
      721 gatatggctt agcgcctctt tcaccatctg ttcaagcatc ggacgcacat catccggggt
      781 gagactttta ccgtccgccg gctgcggaat atttgcgacc gcatcattca ccgccttctg
      841 cagtacttcc ggatcgtagt cacgaccgtc acgcggaaca ggaatatggc ttaccgcctc
      901 cttcactatc tgttcaagca tcggacgcac atcatcgccc gtcacgcact tctgtaatac
      961 cacagacagg gaagccagtt tttcttcaaa cgtttgtgcc tgcgcggcta ttttctcctc
     1021 aaatgtgcgc tgtaaatccg ccagcaccgc ggaaaattct tcacccagcg cacggataat
     1081 ggacagttca cgttcattca ttttttcaga atccccctga acatcgcctt caccgcgtca
     1141 tgctctgttt cagtgattgc cttattaccg tcagatgcag cgaccgcctg aggcacggaa
     1201 gctgttttac cggacgacgc gaacggatcc tcacgggcat cacggcggga caacgcttcc
     1261 agactgtagt tctgctgctg aagatacagt gcatcgcctc ctgccagggg cggcaggttc
     1321 tcccgttttc gggcttcatt gggcgtgaga agcgtatttt tcaccgcatc ccccagcgtt
     1381 ttcatgcgcc gctcactgtc cattctcagc agcgtggtga catcaaattc cgtactctcg
     1441 ttttcccccg tttccagcgc ctcatccagt aacagctcaa tggactcaat cagtgtctgc
     1501 aggcactggg aataatactg ctgctccagc gcctccacgt tgtcactgga aggcggttgt
     1561 cccacgccaa tcttgtaggc cgggacacgg aacaccgaac agacaatttc agcggtcatc
     1621 ttcagttgtt ccaccgtctg cgcatccacc ggtgaaaacg tcgtggggtt gtattttgcc
     1681 ccgttgctca gaatggccgt tttccccgca ttttcgcctg tatacccgct gtcccagttg
     1741 ctcttcagtt ttttcgcatt ttcttccgta atactgccgg ggatctcaat caccccggac
     1801 ggcctgccgc catttctgaa aaaagacgtc gaattttcct gaatatgatg cccctgcgtg
     1861 gccgccagcc cggcggcata caccggcggc aatcctataa gcggatgaaa aaaacagtta
     1921 aaccggtcgt ggatcacttc ccgggcaggc accgtcaccg cctcagtgat cccgcagttc
     1981 cggtccggcg tgatgcggta gaacacctcg ccgtcatccg ccaccagagg ttcaacccgg
     2041 ttccagtcca gaatacgcag ttctttgatc tgcccccggg cattacggat tttcagcacc
     2101 accgtattgc cgtgacgcag tttggcgttc agccacagtt caaaaaactg gatgcggttc
     2161 tgctgggcgt tgggacgacg acagaggcgg gcaatatccc cccggcgcgt ttccctgcgt
     2221 atcccctgcg catccgtctg cataagacgc agccgcattt tggcgatatc ctgggatatc
     2281 agcgaaatac atgcaaacac cgcatgaaag gagaggacgg cttcaggatc ggctttcacg
     2341 ccctgctgcc aggcaccgga aaagggctca gccaccgcct gaaacaggct ggtccagccc
     2401 gcctctctta cgtcacgtcc tgatttctgg ttttttcggg ttcgccgtaa aaggttccac
     2461 attcgccatg ctccgcatca cgtttctttt tctgacctgc cggacgtcgc accgtgatgt
     2521 actccgcctt tcccaggcga accagcacct ccgcacacgg ctgtgccaca tcacggatat
     2581 ccccggcccg ggcatcatgc gtgccctgca gatatcggat ctttgccata acctgttacg
     2641 ggaggcgcac gcctcccgtc ctccttatca gactcagccg ccggacgcac tgccgtagtt
     2701 cactccggtg atcaccgcca ccgccgcggt acggcgacga cgccagttga tccagcgctc
     2761 cgcacggatg gccacgctgc ctgtctggaa catggaaacc agctccaccg gcgacggcgt
     2821 ggtactgtcg ccggtcggct cagactgcat ttccagtgat gcctcgcggg acatatccac
     2881 tgccacgccg ccgtcatccg ccagataaat atccggggca ttcaccagca ccagctggtc
     2941 acccacgtac tgggagacaa tcaccggcag cccctggaag gagccaccca gcagggtcat
     3001 gtccgggtat tctttctgac ccagcgcatt tttacgcatg gacagtgcca gggcattggt
     3061 gctggacatc agccagaccg caccggtggg ctgcaggttt gctgccacaa actgtccaaa
     3121 cgcagcctct gcatccgcat ccgggttacc gcttgatgcc gtgcccttca catcatgggt
     3181 gatggacgcc ggggagacat ctgccaccgc cgcttttttc gggtccacaa agtctgtatc
     3241 cagacgcgcc accaccgctt ccgccagcgc attacggacc agtgcatcag cagccggact
     3301 ggaaaaacag atcaattctt ccgtcagtac cgcaatggcc gacaccttcg catgactgaa
     3361 ggtgatggat tcaaaatcaa acttcgtcag gggttttgcc ttaccctcac ccacccagcc
     3421 ggcagcaccg ccggacacct gggcgtgcac acggatattg aacggcacct gacgaagtgc
     3481 agggatcccg ccctgaccaa atcgcccgat aatggtctga ggacgcaggt aatcaataaa
     3541 gtcctgtgcg tattcctgat attcagacag gctgcctgcc cactgcggat ccgtggtggt
     3601 ccccgcgccc actgccgatt tcaggacatg atgcagacga ctgtcatccg gatactgacg
     3661 acgggccact tccagggctt cagagcggac acctttagcc gcagccagtg atttggcaaa
     3721 acgtgcgaaa ccaatcccct tatccagttt ctgctccaca cggatcaccg gcgcagaagc
     3781 caccgcggcc acattcccgt taccggcctg tttcaccggc tgcgccgtgg cggccttacc
     3841 ggcttccagt tcacgcaggc gcttcaggtg cgcatccacc tgacggattt ccgctgcggt
     3901 gttgtcgtaa tgctcttcct cctccaca
//
LOCUS       DAZGVQ010000280         3913 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000280 DAZGVQ010000000
VERSION     DAZGVQ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.283"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(75..722)
                     /gene="ychE"
                     /locus_tag="ACHLAG_005117"
     CDS             complement(75..722)
                     /gene="ychE"
                     /locus_tag="ACHLAG_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFP0658177.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(753..806)
                     /gene="ychE"
                     /locus_tag="ACHLAG_005118"
     CDS             complement(753..806)
                     /gene="ychE"
                     /locus_tag="ACHLAG_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFP0658178.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1199..3874
                     /gene="adhE"
                     /locus_tag="ACHLAG_005119"
                     /gene_synonym="adhC"
     CDS             1199..3874
                     /gene="adhE"
                     /locus_tag="ACHLAG_005119"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFP0658179.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1074 a          978 c          920 g          941 t
ORIGIN      
        1 taactgcgcg ggaacaaagt aaaaataatg acgaagtaaa cggagcatgg cagctccgtt
       61 tcatttgaaa ggaattaatt aagcaggccg gggaaaatcc ccttaatacc agtaacgata
      121 aattcaatcc ccaatgccat cagcaatagc cccataatac gcgtaatcac gttgatgccg
      181 gtctggcgta aaacccgtac cagccacggt gccatgcgga acaatcccca gcaacataaa
      241 gcgaacaatg caatagccac aaagaaacca aacagatagc taatgctgtg ataacgcgta
      301 ccccagacga tggtagaact gatcgcccct ggccccgcca tcaacggcaa cgccaatggc
      361 accacaccaa tgctttcacg taccgcggtt tctgattttt cttgcttgtt ctgtttatcc
      421 tcgccaagct tgccgctgat catcgacatc gctatcgtca ccaccaggat gcccccggcg
      481 atacggaacg aatcaattga tataccaaaa agttgcagaa tcgtgtcgcc gagaaaaagc
      541 gagatccaca agataatggc cacagacagg ttggctgtaa ggttagtttt gtttcgcgct
      601 gccgctgtct gataactggt catgctgata aagacgggaa taatccctac cgggttgacc
      661 agcgcaaata acccgatgaa aaatttgaag taaacgggaa aatcaaaaaa ggtctgaatc
      721 acggttagct ccgaagcaaa agccggataa tgttagccat aaataaggtt gaaaagacgc
      781 gctgacaata cgccttttga cagcattttt cacctcctaa ctacttaaaa ttgctatcat
      841 tcgttattgt tatctagttg tgcaaaacat gctaatgtag ccaccaaatc atactacaat
      901 ttattaactg ttagctataa tggcgaaaag cgatgctgaa aggtgtcagc tttgcaaaaa
      961 tttgatttgg atcacgtaat cagtacccag aagtgagtaa tcttgcttac gccacctgga
     1021 agtgacgcat tagagataat aactctaatg tttaaactct tttagtaaat cacagtgagt
     1081 gtgagcgcga gtaagctttt gattttcata ggttaagcaa atcatcaccg cactgactat
     1141 actctcgtat tcgagcagat gatttactaa aaaagtttaa cattatcagg agagcattat
     1201 ggctgttact aatgtcgctg aacttaacgc actcgtagag cgtgtaaaaa aagcccagcg
     1261 tgaatatgcc agtttcactc aagagcaagt agacaaaatc ttccgcgccg ccgctctggc
     1321 tgctgcagat gctcgaatcc cactcgcgaa aatggccgtt gccgaatccg gcatgggtat
     1381 cgtcgaagat aaagtgatca aaaaccactt tgcttctgaa tatatctaca acgcctataa
     1441 agatgaaaaa acctgtggtg ttctgtctga agacgacact tttggtacca tcactatcgc
     1501 tgaaccaatc ggtattattt gcggtatcgt tccgaccact aacccgactt caactgctat
     1561 cttcaaatcg ctgatcagtc tgaagacccg taacgccatt atcttctccc cgcacccgcg
     1621 tgcaaaagat gccaccaaca aagcggctga tatcgttctg caggctgcta tcgctgccgg
     1681 tgctccgaaa gatctgatcg gctggatcga tcaaccttct gttgaactgt ctaacgcact
     1741 gatgcaccac ccagacatca acctgatcct cgcgactggt ggtccgggca tggttaaagc
     1801 cgcatacagc tccggtaaac cagctatcgg tgtaggcgcg ggcaacactc cagttgttat
     1861 cgatgaaact gctgatatca aacgtgcagt tgcatctgta ctgatgtcca aaaccttcga
     1921 caacggcgta atctgtgctt ctgaacagtc tgttgttgtt gttgactctg tttatgacgc
     1981 tgtacgtgaa cgttttgcaa cccacggcgg ctatctgttg cagggtaaag agctgaaagc
     2041 tgttcaggat gttatcctga aaaacggtgc gctgaacgcg gctatcgttg gtcagccagc
     2101 ctataaaatt gctgaactgg caggcttctc tgtaccagaa aacaccaaga ttctgatcgg
     2161 tgaagtgacc gttgttgatg aaagcgaacc gttcgcacat gaaaaactgt ccccgactct
     2221 ggcaatgtac cgcgctaaag atttcgaaga cgcggtagaa aaagcagaga aactggttgc
     2281 tatgggcggt atcggtcata cctcttgcct gtacactgac caggataacc aaccggctcg
     2341 cgtttcttac ttcggtcaaa aaatgaaaac ggctcgtatc ctgattaaca ccccagcgtc
     2401 tcagggtggt atcggtgacc tgtataactt caaactcgca ccttccctga ctctgggttg
     2461 tggttcttgg ggtggtaact ccatctctga aaacgttggt ccgaaacacc tgatcaacaa
     2521 gaaaaccgtt gctaagcgag ctgaaaacat gttgtggcac aaacttccga aatctatcta
     2581 cttccgccgt ggctccctgc caatcgcgct ggatgaagtg attactgatg gccacaaacg
     2641 tgcgctcatc gtgactgacc gcttcctgtt caacaatggt tatgctgatc agatcacttc
     2701 cgtactgaaa gcagcaggcg ttgaaactga agtcttcttc gaagtagaag cggacccgac
     2761 cctgagcatc gttcgtaaag gtgcagaact ggcaaactcc ttcaaaccag acgtgattat
     2821 cgcgctgggt ggtggttccc cgatggacgc cgcgaagatc atgtgggtta tgtacgaaca
     2881 tccggaaact cacttcgaag agctggcgct gcgctttatg gatatccgta aacgtatcta
     2941 caagttcccg aaaatgggcg tgaaagcgaa aatgatcgct gtcaccacca cttctggtac
     3001 aggttctgaa gtcactccgt ttgcggttgt aactgacgac gctactggtc agaaatatcc
     3061 gctggcagac tatgcgctga ctccggatat ggcgattgtc gacgccaacc tggttatgga
     3121 catgccgaag tccctgtgtg ctttcggtgg tctggacgca gtaactcacg ccatggaagc
     3181 ttatgtttct gtactggcat ctgagttctc tgatggtcag gctctgcagg cactgaaact
     3241 gctgaaagaa tatctgccag cgtcctacca cgaagggtct aaaaatccgg tagcgcgtga
     3301 acgtgttcac agtgcagcga ctatcgcggg tatcgcgttt gcgaacgcct tcctgggtgt
     3361 atgtcactca atggcgcaca aactgggttc ccagttccat attccgcacg gtctggcaaa
     3421 cgccctgctg atttgtaacg ttattcgcta caatgcgaac gacaacccga ccaagcagac
     3481 tgcattcagc cagtatgacc gtccgcaggc tcgccgtcgt tatgctgaaa ttgccgacca
     3541 cctgggtctg agcgcaccgg gcgaccgtac tgctgctaag atcgagaaac tgctggcatg
     3601 gctggaaacg ctgaaagctg aactgggtat tccgaaatct atccgtgaag ctggcgttca
     3661 ggaagcagac ttcctggcga acgtggataa actgtctgaa gatgcgttcg atgaccagtg
     3721 caccggcgct aacccgcgtt acccgctgat ctccgagctg aaacagatcc tgctggatac
     3781 ctactacggt cgtgattatg tagaaggtga aactgcagcg aaaaaagaag ccgctccggc
     3841 taaagctgag aaaaaagcga aaaaatccgc ttaatcagta gcgctgtctg gcaatataaa
     3901 cggccccttc tgg
//
LOCUS       DAZGVQ010000281         3852 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000281 DAZGVQ010000000
VERSION     DAZGVQ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.284"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1035)
                     /locus_tag="ACHLAG_005120"
     CDS             complement(<1..1035)
                     /locus_tag="ACHLAG_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP0658180.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGLADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     PFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAPLARYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQ"
     gene            complement(1102..3210)
                     /locus_tag="ACHLAG_005121"
     CDS             complement(1102..3210)
                     /locus_tag="ACHLAG_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP0658181.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQASLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIQTVGVNQIETVGSNQIINVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
     gene            complement(3656..>3852)
                     /locus_tag="ACHLAG_005122"
     CDS             complement(3656..>3852)
                     /locus_tag="ACHLAG_005122"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658182.1"
                     /translation="LHIIGGAGENIILGTMNQVLLPLKQDHPGTWWWCSALEMVPSGE
                     LSLFSMLFAYDIHQVNLINS"
BASE COUNT          828 a         1147 c          993 g          884 t
ORIGIN      
        1 ctgcgcgtca tacgtgaaag cgcgcacctg cgtattgctg cggtcatata ccgccagcag
       61 ttcaccggct tccgtatacg tgtaccgcgc cagtggcgca ccgggcaggc tttccgggta
      121 tgccgggtcg tgcatcagcc acaccgccga aagacggatg cccctgtcgg ggccgtattc
      181 ggtaccgggc agtgtgtcgg ggaacggtga ggctgagaga gggcgggaac tgtcagaaga
      241 agatagcgaa gaggtgcggg cctcttccgc ccgctgtgcc tgtgtggtca gtaccagacg
      301 gaactcacgc ccggcaccgt ccgtcacgcc ggtgatttcc ccggccaggt cgcctgcggc
      361 ctcacgccgg tacgtcagcg tccgcccgaa acggtctgcc agcccggtaa gcacccggta
      421 cggcggcagc ggcgctggca gtacgtcctc agcacccggc acccgctctg accaccccag
      481 tatccaccac ggcccctgtg cgctgttggt cgccaggtaa agatgcgggc ttaaccggat
      541 atccggcggc agcgcccccc acagccgcgc cagcgtgtgg ccgtccggct gcgctgcctt
      601 accaccgcgc accagccaca ttgactcgct gcggctgtac accgcctccc ccggcagcag
      661 cggctcaaag tgaatgctcc gcccgccgtt gtcgttgagt accagtgcat catcgcgtag
      721 ctgtaagcgg atatcagaag gcgctttcca gccggggcca aacactccca ccggcgcggg
      781 tgtcctggtc cggtagctgc tgtaggtgcg ggagagaatg aacggcagcg ggccgggcag
      841 cgcaaggtcc gtctcgccgg gcagcacctt cgcccccagc agcggattta ccgggttgcc
      901 cgaagtcatc ccgcccggac acaccgagca cgccacgccg gtgggcgcgc caattcttac
      961 acctgccgaa ccctggacaa taggaccgcc atactgagtc atatctccct gacgcgccgc
     1021 tggttttccg ctcatgattc aattccttct tctgtttatt tttaatgctt gtttgttact
     1081 gaataccgaa caggtacggg atcagtactc tctcatatcc ggcgtatcct gcttttcatc
     1141 aggggtcttc ggtggggatt tcgaggctgc acagttccag ttaatcaaca gtgagtcacc
     1201 attaacctgc tccttaccct gcaaatgaat ttttgtgccg ttgagaaaaa tttgtccgtc
     1261 cttcgtcagc accagccttg ccttaccaca gcagagctcc agatgctcac cggcggagta
     1321 aatcgcggtc tgcccggtat catccttttt cgttttacca acggtgaagg taacgttatt
     1381 tccgactttg acgtcataac ccagcccgac cctcaacgat ttatgcaaac cgatctgcga
     1441 ggactgcatc agagccaccg aagtgttcat aatgccacct accgtcgtct ggtacgccac
     1501 gcccacggtc agcgcacgaa caagtccaat cgtttccacc tgaacagaac ccacgttaat
     1561 gatctggtta ctgcccaccg tctctatctg gttaacgccc accgtctgta tctgattgtt
     1621 ggtaaccgca atcatctgat tgttgccaat ggtctcagca tggttgttta tcacatccgt
     1681 ggtgcggtta ttcagcacct cggtgttcat gttcttctgc gcgtggatgt agacctgttc
     1741 tttccctgtc gcatcgtcaa acttcagttc attaaacccg ctgcccttat aggttttcga
     1801 acgaatggtc atctgcgtct tcgtccccgg caggctgccg ggtgtgcggt tttcctggtg
     1861 gtaggtacgc cccataataa tcggctggtc cggatcgccg ttgaggaagt ccacaatcac
     1921 ctcctgaccc acacgcggta tcgccaggtg gccaaaaccg gtgcctgccc acgcctgtgc
     1981 cacacggatc cagcacgaac tgtcttggtc tgccgggtta taacggtccc agttgaactt
     2041 cacccgcacg cgaccatgtt cgtcgcagaa gatttcctcg cctgccggtc ctgtcacgac
     2101 agcgctctgc gggccgtcga ccagcggttt cagcaacggc tgtggtcgcc atgttctgtc
     2161 tgccgggatc accgcaaaat ggttctccag cgccgtcccc gctccctgcc gtcctggcac
     2221 cgcctgtggc tgttcgccgt gcagttcact tgccaccacc tgccattccc ggttcaggct
     2281 cgcctgcgga tgccccgtca gcacaattcg tcgtcccggc catatctccg gcgagcgact
     2341 cattccccgc gcggtttctg cattgtttcg ccagccgtcc atctgccagc gggcaaagtt
     2401 ctgcccgtgg gcacccttga aacgtccggg gtagtcatac acttcatact gcgtgcgctg
     2461 gtaatcctgg tactggcctt cctgatcaaa acgccctgcc cagccgggtc gtttaaaggt
     2521 gtagtctttg gtcaccacgg aagaagggcg gatttgtgca ctgtagcgga actggctgat
     2581 gcagagggtg ctcacctcgg tacgggtgtt cggattccag gggatctcaa aggactccgg
     2641 cagataacgc acggtgtcgc acaggaccag gctctggtcg gtactttttt gcgcgtgctc
     2701 ctcataaaag aagatgcctt cctccgccgc catccggcac aggaaatcgt aatcggtttc
     2761 gccgtactgg acacaaaact cacgggaagg atgcggctcg ctgaacagcg gactccactc
     2821 ggtcacaccg ttttccttca ggatcgtagc gaggatgctt tcgatgtcct cattctggaa
     2881 gatacggaag ttctggcgca gccctgtgcg ccacagcggc gggcacacct tcatgctgta
     2941 cagcatctgg tttttgtcgt tctcccccag ttcaaaccag gtcaccacac ctttcacccg
     3001 gcgctgtacg tcatcgccct gccataccgt caggtaggcc attttgtcca gcacctgcgc
     3061 aaattcaagg gaaagaaact gctgactgac cagagaaagg tcgagggaaa aaagtgaaga
     3121 gagtgactgg gtgagatgaa aggaaaccac cgcaaacgcg tccggtggca ggccgtccac
     3181 ttcaagtgtg aaacgtaatc cggttgacat agcaacctcc gtgaaaatga ataataatcc
     3241 gcacatattg catgtacaac atgtcgggcg gatgagataa gcgagtcgac atctggcgtt
     3301 accggggaat atcagaaaaa tgtcatggtt atgtgatgat ttagtctggg attttacttc
     3361 agagaaacgg gtgtctgttt agtcaacaaa ataatcacaa acgcaacgga tgggttctcg
     3421 acgttattca cgacaggctc gtacattaca gcgagtaata aaaaacctga tatgcaaagt
     3481 caaaccaata ttcataatgt ctttttattc acaacaataa ataattcaat aacaatcatt
     3541 gaaatgaatg atgatttgta ttaggaatat acgctaccgc cccaccctga taacatgatc
     3601 tgcacattgc catcttaacg tcgttaaaca ctggcttaat agtggatatt tcaaattagg
     3661 aatttattaa attcacctga tgaatatcat aagcaaatag catagaaaat aatgacaatt
     3721 cccccgaggg gaccatttcc aaagcactgc accaccacca ggtgcccgga tgatcttgct
     3781 tcagtggtaa gagtacctga ttcattgttc ccaaaatgat gttttccccg gccccgccaa
     3841 taatgtgcag ct
//
LOCUS       DAZGVQ010000282         3769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000282 DAZGVQ010000000
VERSION     DAZGVQ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.285"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..472
                     /locus_tag="ACHLAG_005123"
     CDS             <1..472
                     /locus_tag="ACHLAG_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HFP0658183.1"
                     /translation="KAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFDESKMHL
                     FTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLESQKITLNNNIV
                     DDDQGIFVMCDYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRIFLSRK"
     gene            747..1943
                     /gene="kbl"
                     /locus_tag="ACHLAG_005124"
     CDS             747..1943
                     /gene="kbl"
                     /locus_tag="ACHLAG_005124"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFP0658184.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPYLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            1953..2978
                     /gene="tdh"
                     /locus_tag="ACHLAG_005125"
     CDS             1953..2978
                     /gene="tdh"
                     /locus_tag="ACHLAG_005125"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFP0658185.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            3217..>3769
                     /gene="waaH"
                     /locus_tag="ACHLAG_005126"
     CDS             3217..>3769
                     /gene="waaH"
                     /locus_tag="ACHLAG_005126"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFP0658186.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFI"
BASE COUNT          924 a          836 c          998 g         1011 t
ORIGIN      
        1 taaagctatc aacatgggct tagtgaaaac gcctcttgtt gcgtggatag attttggcta
       61 ttgccgtaag ccaaatgtaa ctcgaggatt gaaaatttgg gatttcccat ttgatgaaag
      121 taagatgcat cttttcacta taaaaaaagg gcttactgtc acatctcaac aacaggtttt
      181 tgatttcatg attggcaatc atgtatatat tattgggggc gcaattgttg gttcacagca
      241 taaatggaaa gaattctata aattagttct ggaaagtcaa aaaataacac taaataataa
      301 cattgtggat gatgaccagg gcatttttgt catgtgcgat tacaaacgcc ccgatctctt
      361 caacctaaac tatctgggga gaggaaaatg gttcgatttg tttcgctgct tcaggagtaa
      421 cactttaggg gcaaaaatgc aggcgctgag aattttcctt tcaagaaaat aggtttccct
      481 ccattagtgg gtagcgtttt gataccaact gagataggaa aaatgatgct ttggcatcat
      541 tttttctttt tatatcaatg cagtaaaatc aatcatcatt atgcccagcc aaaactgtac
      601 agtttttcta ctatctgtag gtgttgagag acatcaagtt acacgttatt tatcctgaat
      661 tttgcagaag tgttaacgcg tcatctcgtc gcgacctata agtttgggta atatgtgctg
      721 gaatttgccc tgtctggaga atcgcaatgc gtggagaatt ttatcagcag ttaaccaacg
      781 atctggaaac cgcacgggcg gaagggttgt ttaaagaaga gcgcattatt acgtctgcgc
      841 agcaagcaga tatcactgtg gctgatggaa gccacgtcat taacttttgt gccaataact
      901 atctcgggct ggcgaatcat ccgtatctga ttgcggcggc aaaggcggga atggattctc
      961 acggtttcgg catggcttcg gtgcgtttta tttgcggcac tcaggacagc cataaagagc
     1021 ttgaacaaaa actggcggca ttcctgggga tggaagatgc gattctctac tcttcctgct
     1081 ttgatgctaa cggtggcctg tttgaaacgc tgctgggcgc ggaagacgcc attatctccg
     1141 acgcactgaa ccacgcgtct attattgatg gtgtgcgtct gtgtaaagct aaacgctatc
     1201 gctatgccaa caacgatatg caggagcttg aagctcgtct aaaagaagcg cgtgaagccg
     1261 gtgcgcgtca tgtgctgatt gccaccgatg gtgtgttctc gatggacggc gtgattgcca
     1321 acctgaaggg cgtttgcgat ctggcagata aatacgatgc cctggtgatg gtagacgact
     1381 cccacgcggt cggttttgtc ggtgaaaacg gtcgtggttc ccatgaatac tgcgatgtga
     1441 tgggccgggt cgatattatc accggcacac ttggtaaagc gctgggcggg gcttctggtg
     1501 gttataccgc ggcgcgcaaa gaggtggttg agtggctgcg ccagcgttct cgtccgtacc
     1561 tgttctccaa ctcgctggca ccggccattg ttgccgcgtc catcaaagta ctggagatgg
     1621 tcgaagcggg cagcgaactg cgtgaccgtc tgtgggcgaa cgcgcgccag ttccgtgagc
     1681 aaatgtcggc ggcgggcttt accctggcgg gagccgatca tgccattatt ccggtcatgc
     1741 ttggcgatgc agtagtggcg cagaaatttg cccgtgagct gcaaaaagag ggcatttacg
     1801 tcaccggttt cttctatccg gtcgttccga aaggtcaggc gcgtattcgt acccagatgt
     1861 ctgcggcgca tacccctgag caaattacgc gtgcagtaga agcattcacg cgtattggta
     1921 aacaactggg cgttatcgcc tgaggatgtg agatgaaagc gttatccaaa ctgaaagcgg
     1981 aagagggcat ctggatgacc gacgttcctg taccggaact cgggcataac gatctgctga
     2041 ttaaaatccg taaaacagcc atctgcggga ctgacgttca catctataac tgggatgagt
     2101 ggtcgcaaaa aaccatcccg gtgccgatgg tcgtaggcca tgaatatgtc ggtgaagtgg
     2161 taggtattgg tcaggaagtg aaaggcttca aaatcggcga tcgcgtttct ggcgaaggcc
     2221 atatcacctg cggtcactgt cgcaactgtc gtggcggtcg tacccatctg tgccgcaaca
     2281 cgatcggcgt cggcgttaac cgtccgggct gcttcgccga atatctggtg atcccggcgt
     2341 tcaacgcctt caaaatccca gacaatatct ccgacgacct ggcttccatt tttgatccct
     2401 tcggtaacgc cgtgcatacc gcgctgtcgt tcgatctggt gggcgaagat gtgctggttt
     2461 ctggtgcagg tccgataggt attatggctg cggcggtggc gaaacacgtt ggtgcacgca
     2521 atgtggtgat caccgatgtt aacgaatacc gccttgagct ggcgcgcaaa atgggtatca
     2581 cccgtgcggt taacgtcgcg aaagaaaacc ttaatgatgt gatggctgaa ctgggcatga
     2641 ccgaaggctt tgatgtcggt ctggaaatgt ccggcgcgcc gccagcgttt cgtaccatgc
     2701 tcgataccat gaaccacggc ggtcgtattg cgatgctggg tattccaccg tccgatatgt
     2761 ctatcgactg gaccaaagta atctttaaag gcttgttcat taaaggtatt tacggtcgtg
     2821 agatgtttga aacctggtac aagatggcgg cgctgattca gtcaggtctg gatctctcgc
     2881 cgatcattac ccatcgtttc tctatcgatg atttccagaa gggctttgac gctatgcgtt
     2941 cgggccagtc cgggaaagta attctgagtt gggattaaca cgaacaaggg ctggtattcc
     3001 agcccttttg cctgaggata atccgttaaa tatgtaaaat cctgtcagtg taataaagat
     3061 atcgtaattg cgctgatctc ttatatagct gctctcatta tctctctacc ctgaagtgac
     3121 tctctcacct gtaaaaataa tatctcacag gcttaatagt ttcttaatac aaagcctgta
     3181 aaacgtcagg ataacttcag aggtcgtcgg taatttatga tgaacagcac caataaactt
     3241 agtgttatta ttccgttata taatgcgggc gatgatttcc gcacttgtat ggaatcttta
     3301 attacgcaaa cctggactgc tctggaaatc attattatta acgatggttc aacggataat
     3361 tctgttgaaa tagcaaagca ttacgcagaa aactatccgc acgttcgttt gttgcatcag
     3421 gcgaatgctg gcgcatcggt agcgcgtaat cgtgggatcg aggtggcgac gggcaaatat
     3481 gtcgcttttg tcgatgctga cgatgaggtc tatcccacca tgtacgaaac gctgatgacc
     3541 atggcgttag aggatgacct cgacgtggcg cagtgcaacg ctgactggtg ttttcgtgaa
     3601 acgggagaaa cctggcaatc catccccagc gatcgccttc gctcaaccgg cgtattaacc
     3661 ggcccggact ggctgcggat ggggctttct tcgcgccgtt ggactcacgt tgtctggatg
     3721 ggggtttatc gccgtgatgt tattgttaaa aataacatta aatttattg
//
LOCUS       DAZGVQ010000283         3716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000283 DAZGVQ010000000
VERSION     DAZGVQ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.286"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..594)
                     /locus_tag="ACHLAG_005127"
     CDS             complement(<1..594)
                     /locus_tag="ACHLAG_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658187.1"
                     /translation="MYGLSIMKPDGSVWISPGFTPQCLINKGTIPATEKSFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHVIVRGTNPGVTTVYAFANMVTPPSEY
                     GIAMYNPDGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYIDKVLV"
     gene            complement(600..878)
                     /locus_tag="ACHLAG_005128"
     CDS             complement(600..878)
                     /locus_tag="ACHLAG_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658188.1"
                     /translation="MEYGFAIYNRNNVNVTGVLTPVFFLDRFTAESGSKTYTNKPDGK
                     SLQAVCCLFPWNNVFADRKVPKITINGNTVTWSNLEQGMGSYIYTFWG"
     gene            complement(893..2161)
                     /locus_tag="ACHLAG_005129"
     CDS             complement(893..2161)
                     /locus_tag="ACHLAG_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP0658189.1"
                     /translation="MTRKPWRAGKDLSTVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGASYALNPGIEIDSTLMTVDFPTKPLNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLVATTPGQVYGDPVDPGWSGFYWIRFVNAAGVKGPWNAEKGTQ
                     AQTQIGVKAIIDQIRDEAAKSPVVSELRKEIKNAQGQAVKDAAIKTTEVVGTLREETT
                     RTIGGIETRISTLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGI
                     VAGKDSEGRPVSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETL
                     VSRKVVADEVKAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYS
                     NQNVGLVIRNDKIEVYDQNGRLAVRIGRLR"
     gene            complement(2158..>3716)
                     /locus_tag="ACHLAG_005130"
     CDS             complement(2158..>3716)
                     /locus_tag="ACHLAG_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658190.1"
                     /translation="HSAVLAENCHFRYGVITPEHQMSEAEKTFAIKPKTIFHYRDDFW
                     FAWTDVVDVIRSPVAQDSHGRIYYTDGRFPKVTDATIATKGDGNHPTSSYRLGIPAPT
                     TAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYGEEGPPGPASLEVTLRTPGTAVQL
                     TLSPVPLQNASIKRRRIYRSASGGGEADFLLVAELDASVLSYTDKIPGKNLGPSLATW
                     DYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYLPYAWPEVNRHTTAEDIVAICPLG
                     TSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLSRRSMVAMEGFVLYAGTNGLVSVD
                     VNGNTALATEKIISPEQWQSQFNPASIVAYSWRGEYIACYTKPDGKQDVFVFSPVNMD
                     IRYLSTPFDCAWVDLAKDMMRVVTGDKMSVLAGSSLPSTIRWHSKIFSLPERTSFSCI
                     RVKSPAPERVGITIMADDVPVIHFAPGTFKGSVVRLPAATGQNWQVMVSGFGQVERIT
                     LSTSMSEMPV"
BASE COUNT          946 a         1046 c          779 g          945 t
ORIGIN      
        1 aaccaggacc ttatctatat acggctttcg aatatcccat gttgcaccgc cggaataatg
       61 ctcacaggaa tatattttat ttcctgtagc accagtggat gtggtataaa tcggtcgatc
      121 ataaggagtt cttttccagt tataataccc gaccagtgca ggcatgattg cgcatggata
      181 tccaaggtcc ttttcaaatt tgatatcaac aggtattaac ttcgcgtcaa gcagcatcat
      241 ttcgccatga taaatcatct caccgtccgg gttatacatg gcgataccat actcagaagg
      301 tggagtaacc atattcgcga aagcataaac cgtcgtaaca ccagggttcg ttcccctgac
      361 aattacatga agccttagtg catgatatcc atcaatctgt tcatgcgtgt acatgacatc
      421 ggccttcttc tctgttctga taaagaaaaa acaacttttg cctgacggga ttgatgtttt
      481 aaaaaaagac ttttcagtcg ccggtatggt gcctttgttg atcagacact gcggcgtaaa
      541 acctggactt atccatacgc tgccatccgg cttcataatg ctcaaaccat acatgacact
      601 tatccccaga atgtataaat ataagatccc ataccctgct caagattcga ccacgtcacc
      661 gtattgccat taatggttat cttcggtact ttccgatccg caaatacgtt attccaggga
      721 aataaacaac atacagcctg caatgatttc ccgtcgggtt tattcgtgta cgtctttgag
      781 ccagactccg ctgtaaatct gtccaggaaa aataccggag tcagcacgcc cgtaacatta
      841 acgttatttc tgttataaat ggcaaaaccg tattccaata ctcacctcct gatcagcgta
      901 atctgcctat gcgaacagcc agtcgtccat tctgatcata aacctcaatt ttatcattgc
      961 ggatcaccag tcctacattc tgattagagt aacgaattgt cagttgccct tgtgacgtaa
     1021 cactgaaaag gcctccaata ttcaggttac cctgagaatc aacctgaaag tttccgttct
     1081 gaataacggc actccggata actggcgaag tgatacttac cccggctttt acctcatccg
     1141 ccacaacctt ccgcgacacc agtgtttcaa tcaccgcgtc ataaatcatc gctttcggga
     1201 tcacaacctt gccacctgat accgcaaacg gataggcggt gttatccggg ttgttcgggt
     1261 caaagacaaa caactgcgac gcagaaattg caacctgact tacaggccta ccttcactgt
     1321 cttttccggc gacaatcccg atccccgcag tgataccatc aactcccgct ttttttgacc
     1381 acattgccag aaacgcctca ccgccttctt tatccagttt agtgatgcgc ttgtcgacct
     1441 cattaagcga ttcactggtt gatgaatcca gtgtgctaat gcgggtttca ataccaccaa
     1501 tcgttcttgt cgtttcttcc ctgagagtcc ccacaacttc ggttgtctta attgcagcat
     1561 cctttacagc ctgcccctgc gcgtttttta tttctttacg cagctcggac acaaccggcg
     1621 actttgcagc ctcatcgcgg atctggtcaa tgatggcctt cacgccgatc tgtgtttgtg
     1681 cctgagtgcc tttttcagca ttccatggac ctttcactcc tgccgcgtta acaaaacgta
     1741 tccagtaaaa tcccgaccag ccagggtcaa ccggatcgcc gtaaacctgc cccggcgtcg
     1801 tggcaaccag cactgcatca gcaaggtcat cctccgtacc ccgccagatt tcagtcagtg
     1861 aatgtccgcg ataattaggc atatcccatt caagaagaac cgagccaaat cctcctgtcg
     1921 ccttaaaatt cagcggtttt gtgggaaaat caacagtcat taaagtactg tcaatctcaa
     1981 tacccggatt cagtgcatat gaggcaccac ccgatgttcg acgccgggcg agtttaagac
     2041 caaccagttc ctcacgggtc acaaatgcgt ggcgtccgtc accacgctgc ccggtgccaa
     2101 tttccatgtt ctccacaact gtggataaat ccttccccgc acgccacggt tttctggtca
     2161 taccggcatc tccgacatcg atgtactcag ggttattcgt tccacctgcc cgaatccgga
     2221 taccatcacc tgccagtttt gcccggttgc tgccggaagt ctcaccacac ttcccttaaa
     2281 cgtacccggc gcaaaatgaa tcacaggaac atcatcagcc ataatggtga tccccacccg
     2341 ctcaggcgcc ggagatttca ccctgataca ggaaaaagag gttctttcag gtaatgaaaa
     2401 aatttttgaa tgccacctta tcgtggaggg cagagagctc ccggcaagca ctgacatttt
     2461 gtctcctgtc accacgcgca tcatatcttt cgcgagatca acccatgcgc agtcaaacgg
     2521 tgtactgaga taacggatat ccatgttcac cggactgaat acaaacacat cctgcttacc
     2581 atccggtttc gtgtaacagg caatgtactc accacgccag gaataagcca caatggacgc
     2641 cgggttaaac tgactctgcc actgttcagg tgaaataatc ttttccgttg ccagtgctgt
     2701 attaccgttt acatcaacag ataccagacc gtttgtccca gcatagagta cgaatccctc
     2761 catcgccacc atacttcttc ggctcaggca tgcctgcatg gaaggaattc tggagccaga
     2821 aattgtggaa ggcgataccc cactgaacag atagggctcc ccctttgtcg ccaccaccag
     2881 tgacgttccc agcggacaga tagccacaat atcttctgcc gtcgtgtgac gattcacttc
     2941 cggccatgca tacggcagat acgcttccga aaacatcact tcattaccgg caaacccggc
     3001 ggcaatacca ttagccatca ggcaaaggcc tgtcatattc tctggcggcg gcaggtaatc
     3061 ccatgtcgcc agagaaggtc caaggttttt ccccggtatt ttgtccgtgt aactgagcac
     3121 ggatgcatcc agttcagcca caagtaaaaa atccgcttct cctccacctg atgcagagcg
     3181 ataaatccgg cggcgtttaa tactggcatt ctgcaatggc accggagaca gcgtcagctg
     3241 taccgcagtc cccggagtac ggagtgttac ctccagagac gccggacctg gcggaccttc
     3301 ttcaccataa tctgagacaa aggtttccgt ataaaaccgg gtttcgtcat cattcgggtt
     3361 atcgtcagaa acatcaccgc cctgctgaac agtacagaca ggtgctgtcg tcggcgcggg
     3421 gatccccaga cgatacgatg atgtcgggtg attcccgtcc ccttttgtgg caatggtcgc
     3481 atccgtcact ttaggaaaac gcccgtcagt gtagtaaata cgcccgtggg agtcctgagc
     3541 gaccggactg cggatcacat ccaccacatc cgtccatgca aaccagaaat cgtcacggta
     3601 atgaaaaatg gttttcggct taatcgcgaa tgttttctca gcctctgaca tctggtgttc
     3661 aggcgtgatc actccatagc gaaaatgaca gttttctgcc agtacagcag aatgat
//
LOCUS       DAZGVQ010000284         3446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000284 DAZGVQ010000000
VERSION     DAZGVQ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.287"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..450
                     /gene="lpfB-O113"
                     /locus_tag="ACHLAG_005131"
     CDS             <1..450
                     /gene="lpfB-O113"
                     /locus_tag="ACHLAG_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFP0658191.1"
                     /translation="RLEPSQEKILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKAT
                     NSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVD
                     GHDVPLNMIRPFETLTLPLPANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            475..2997
                     /gene="lpfC-O113"
                     /locus_tag="ACHLAG_005132"
     CDS             475..2997
                     /gene="lpfC-O113"
                     /locus_tag="ACHLAG_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFP0658192.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            3008..>3446
                     /gene="lpfD-O113"
                     /locus_tag="ACHLAG_005133"
     CDS             3008..>3446
                     /gene="lpfD-O113"
                     /locus_tag="ACHLAG_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFP0658193.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNC"
BASE COUNT          943 a          747 c          836 g          920 t
ORIGIN      
        1 cgattagaac cttcgcaaga aaaaatatta cgcattattc acaccaaagg tgtgtcattg
       61 cctgatgatc gcgagtcagt cttctggctg aatattaaaa atattccgcc atcagcgagc
      121 aacaaagcga cgaactcgct ggaaatagca gtaaaaacgc gtatcaagct gttctggcga
      181 ccagcaaata tccgccttat tcccgaagat gctgcgccga aagtgaaatg gcgccgggaa
      241 gggcgaaatc tgatagcaga gaatcccaac ccaattcata tcagtgtgat ggatgtcatc
      301 gttgatggcc atgatgtgcc cctcaatatg atccgtcctt ttgaaacgct cacgttacct
      361 ttacctgcta attctgcggg aggccaaatg acctggcgat ttattaatga ttacggcgca
      421 gtcagtgacc cgataaaaat gacgctgtaa gcaagggata aggataggcg tattatgatg
      481 acgaccagaa tagtggttgg cctcacggca gggacgtgtc tgattttctc gcaaaacctg
      541 atggccgagg tcagtgtatt caatccggcg cttctggaaa tcgaccatca atccggagtc
      601 gatattcgcc agtttaatcg ggcaaacctg atgcccccag gtgtttatag cgttgatatt
      661 tttatcaacg gtaaaatgtt tgaacgtcag gatgtgacat ttgttcagga taatccagat
      721 gctgatctgc acgcatgctt tattgccatt aaaaaaacac tgtcctcctt tggcataaaa
      781 gttgatgcgc tcaaatcgtt caatgatgtg gatgagacgg tttgcctcga tcctgctcca
      841 cgtattgaag gctcatcctg gcagtttgac agtgataaat tgcagctgaa tatatccatt
      901 ccccaaatct acatggacgc gatggcttat gattacatca gccccacgcg ttgggatgag
      961 gggattaacg cgctcaccat caactacgat ttttctggtt cacatacact acgttcagat
     1021 tatggttcac aagagacaga taccagttat ctcaatctgc gcaatggact gaatattgga
     1081 ccgtggcggc tacgtaatta cagtacttta aacaccagcg atggccgtgc ggaatacaac
     1141 tccattagta cctggataca gcgcgatatt gccgcgttaa gaagccagat tatgattggt
     1201 gatacgtgga cggcgagcga tattttcgac agtacgcaaa ttcgcggcgc gcgtttgtat
     1261 actgataacg atatgctacc cgccagccag aatggctttg ctcctgtggt tcgtgggatt
     1321 gcaaagtcca acgccaccgt catcattcgg cagaatggct acgtgattta tcagtctgcc
     1381 gttccacaag gtgcttttga gatcaccgat ctcaacaccg ccagtacagg tggcgatttg
     1441 gacgtaacca tcaaagaaga agacggtagc gaacaacgat tcacccaacc ttatgcttca
     1501 ttggcgattc ttaaacgtga aggtcagaca gatgttgatg tcagcgtggg tgaattgcgc
     1561 gatgaagacg gatttacacc ggacgtcctt caggcgcaaa tacttcatgg tttttcccac
     1621 gggatcactt tatatggagg tatgcaggct gctgaaaatt atggttctgc agctctgggt
     1681 gtcggtaaag atcttggcgc tttgggcgca atttctttcg atgtgacaca tgctcgtgcg
     1741 aattttagcc atgatgatac agaaacgggt cagtcatatc gctttctcta ttcaaaacga
     1801 tttgacgaca cagacactag cttgcgcctg gttggctatc gttactccac cgagggctac
     1861 tataccctca atgagtgggc atcgcggcgc aacagccctg aagacttttg ggaaacaggt
     1921 aaccgacgta gtcgcgtgga gggaacgcta acgcagtcgt tggggagaga ttatggcaat
     1981 ttatacctga cattaagccg gcaacaatac tggcataccg atgatgtcga acgattaatg
     2041 caatttggct acagcagtag ctggaagcgt ctctcgtgga acgtctcctg gagttattcc
     2101 aatactgcca gacaggggac ggggaacaac catgccagtg ataacaccag tgagcagatc
     2161 tacatgctct ctttatctgt tcctttatcg ggctggtggg gtaatagtta cgccacctat
     2221 tctgtttcgc aaaacgataa ttccggtagc tcacatcaac tcggactcag cggtacggcg
     2281 ctggaaagaa ataacctttc atggaattta atgcagtcct ataacagtca tgatgatgag
     2341 gttggcggta atatgtccct gacctatgat ggctcttatg gcacggtgaa cggcagctat
     2401 aactacagcc aaaattccca gaggctgaat tatggtatca gagggggaat tctggcacac
     2461 agcgaagggg taacgttaag tcaggagtta ggtgaaacta ttgctcttgt taaagcacct
     2521 ggggccgccg ggttagaaat agataatatg cgcggtgctg cgacggactg gcgcggctat
     2581 acggtcaaga cacagctaaa cccttatgat gaaaatcggg tagcaatcag cgataactat
     2641 ttctcgaagt cgaatataga acttgataat accgtcgtta cgatggttcc cacgcgtggt
     2701 gcagtggtta aagcggagtt tgtgactcat gtgggttatc gcgttctctt cagagtgtta
     2761 aatgcaaatg gtaaaccggt accttttgga gccattgctg cgatacaaga tgcaagtttg
     2821 gcagattcag gaattgtcgg tgaccgtggc gaactttatc tttctggtct accagaaaaa
     2881 ggacaggtta cgttatcctg gggagaaaac gcctcaacaa aatgcatctt caattattca
     2941 ctttcgacac cagaaagtga gagcggatta attgaacagg gtgtgacatg tcattaaaag
     3001 gatttttatg aacaagtata tcaaacagtg gtgctttgct gtgtttatgc tctcgttaag
     3061 tagtgtagca cttgcagctc ctaaaggtat ctgtacccct gataatggcg tatttcacag
     3121 tacgcttgat ttctcgggat atcttattac cgcgaacgaa aataaggttg gaacgacatt
     3181 taacaccact gtaactaatg gatcctcata tcctggacgc tgtcattgtg atactggtaa
     3241 cgtaggagaa ttcccctata tctattatac gtcaaaaata aaccaggcat taacttatgc
     3301 tggggttcac tctaatatta attattatga tcttaatccg aaccttgatg ttgggattgc
     3361 tatagatatt cttggggttg ggtatgtgaa tgcacctttt gaatatcatg ctaacaaccc
     3421 gtctggtaat acaaagtaca attgca
//
LOCUS       DAZGVQ010000285         3303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000285 DAZGVQ010000000
VERSION     DAZGVQ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.288"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            349..1665
                     /gene="shiA"
                     /locus_tag="ACHLAG_005134"
     CDS             349..1665
                     /gene="shiA"
                     /locus_tag="ACHLAG_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFP0658194.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAITALLMKDNQRA"
     gene            1767..3221
                     /gene="amn"
                     /locus_tag="ACHLAG_005135"
     CDS             1767..3221
                     /gene="amn"
                     /locus_tag="ACHLAG_005135"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFP0658195.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPLQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          765 a          815 c          796 g          927 t
ORIGIN      
        1 atgcaatacg tctcaacaat gatgctaaaa acatacctaa cctcgcctcc ctactggtta
       61 taatgcaatg cagtctatca gactcatcag ggtgccattt tgtgcatatg cggactttta
      121 tgtttcatat ctctaacctg tgggtcctct gcttaatcct taaacaacac cagcaactcc
      181 tgcgctttca tcttccatcg aatttttcat gttgccgcta atcagccata aaatcatttg
      241 cagatgcgct ctgtcgaggt agtctcataa ggttcgttta tagatcgacg gcaatgtgag
      301 ttaccttttc catactaatt ataaaaagac agtacaaaca ggatcattat ggactccacg
      361 ctcatctcca ctcgtcccga tgaagggacg ctttcgttaa gtcgcgcccg acgagctgcg
      421 ttaggcagct tcgctggtgc cgtcgtcgac tggtatgatt ttttactcta tggcatcacc
      481 gccgcactgg tgtttaatcg cgagtttttc ccgcaagtaa gcccggcgat gggaacgctc
      541 gccgcatttg ctacctttgg cgtcggattc cttttccgtc cgctcggcgg tgtcattttc
      601 ggtcactttg gcgaccgact gggacgtaag cgcatgttaa tgctgaccgt ctggatgatg
      661 ggcatcgcga cagccttgat tggtattctt ccttcattct cgaccattgg gtggtgggca
      721 cctattttac tggtgacgct gcgtgccatt cagggatttg ccgtcggcgg cgaatgggga
      781 ggcgcggcgt tgctttccgt tgaaagtgca ccaaaaaata aaaaagcctt ttacagtagc
      841 ggtgtacaag ttggctacgg tgtaggttta ctgctttcaa ctggactggt ttcattgatc
      901 agtatgatga cgactgacga acagttttta agctggggct ggcgcattcc tttcctgttt
      961 agcatcgtac tggtactggg agcattgtgg gtgcgtaatg gcatggagga gtccgcggaa
     1021 tttgaacaac agcaacataa tcaagcggcc gcgaaaaaac gcattccggt tatcgaagca
     1081 ctattacggc atcctggtgc tttcctgaag attattgcgc tacgactgtg cgagttgctg
     1141 acgatgtaca tcgttaccgc cttcgcactt aattattcaa cccagaatat ggggttaccg
     1201 cgcgaacttt tcctcaatat tggtttgctg gtaggtggat taagctgcct gacaattccc
     1261 tgttttgcct ggcttgccga tcgttttggt cgccgcaggg tttatatcac aggcgcgtta
     1321 atcggaacgt tgagcgcatt tcctttcttt atggcgcttg aagcacaatc cattttctgg
     1381 atagttttct tctccataat gctggcaaac attgcgcatg acatggtggt gtgtgtgcaa
     1441 caaccgatgt ttaccgaaat gtttggtgcc agttatcgct atagtggcgc tggagtcggt
     1501 tatcaggttg ccagtgtggt tggcggtgga tttacacctt ttattgccgc tgcactcatc
     1561 acttactttg ccgggaactg gcatagcgtc gccatttatt tgctggctgg atgcctgatt
     1621 tctgcaataa ccgctttgtt gatgaaagac aatcaacgcg cttgatagtc tggcgaagat
     1681 catccgatct tcgccttaca cttttgtttc acattcctgt gacatactat cggatgtgcg
     1741 gtaattgtat ggaacaggag acacacatga ataataaggg ctccggtctg accccagctc
     1801 aggcactgga taaactcgac gcgctgtatg agcaatctgt agtcgcatta cgcaacgcca
     1861 ttggcaacta tattacaagt ggcgaattac ctgatgaaaa cgcccgcaaa caaggtcttt
     1921 ttgtctatcc atcactgacc gtaacctggg acggtagcac aaccaatccc cccaaaacgc
     1981 gcgcatttgg tcgttttacc cacgcaggca gctacaccac cacgattact cgccctactc
     2041 tctttcgttc gtatcttaat gaacaactta cgttgctgta tcaggattat ggtgcgcata
     2101 tctctgtgca acccttgcag catgaaatcc cttatcctta tgtcatcgat ggctctgaat
     2161 tgacacttga tcgctcaatg agcgctgggt taactcgcta cttcccgaca acagaactgg
     2221 cgcaaattgg agatgaaact gcagacggca tttatcatcc aactgaattc tccccgctat
     2281 cgcattttga tgcgcgccga gtcgattttt ccctcgcgcg gttgcgccat tataccggta
     2341 cgccagttga acattttcag ccgttcgtct tgtttaccaa ctacacacgt tatgtggatg
     2401 aattcgttcg ttggggatgc agccagatcc tcgatcctga tagtccctac attgcccttt
     2461 cttgtgctgg cgggaactgg atcaccgccg aaaccgaagc gccagaagaa gccatttccg
     2521 accttgcatg gaaaaaacac cagatgccag catggcattt aattaccgcc gatgctcagg
     2581 ggattactct ggtgaatatt ggcgtgggac cgtcaaatgc taaaaccatc tgcgatcatc
     2641 tggcagtgct acgcccggat gtctggttga tgattggtca ctgtggcgga ttacgtgaaa
     2701 gtcaggccat tggcgattat gtacttgcac acgcttattt acgcgatgac cacgttcttg
     2761 atgcggttct gccgcccgat attcctattc cgagcattgc tgaagtgcaa cgtgcgcttt
     2821 atgacgccac caagctggtg agcggcaggc ccggtgagga agtcaaacag cggctacgta
     2881 ctggtactgt ggtaaccaca gatgacagga actgggaatt acgttactca gcttctgcac
     2941 ttcgttttaa cttaagccgg gccgtagcaa ttgatatgga aagtgcaacc attgccgcgc
     3001 aaggatatcg tttccgcgtg ccatacggga cactactgtg tgtttcagat aaaccattac
     3061 atggtgagat taaacttccc ggccaggcta accgttttta tgaaggcgct atttccgaac
     3121 atctgcaaat tggcattcgg gcgatcgatt tgctgcgcgc agaaggcgac cgactgcatt
     3181 cgcgtaaatt acgaaccttt aatgagccgc cgttccgata ataagttctg aaccttctta
     3241 tcagaaatag atgtgaagga gtaagtaaga cacctggcaa atagcctgca ataatcgtgg
     3301 gct
//
LOCUS       DAZGVQ010000286         3277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000286 DAZGVQ010000000
VERSION     DAZGVQ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.289"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..359
                     /locus_tag="ACHLAG_005136"
     CDS             <1..359
                     /locus_tag="ACHLAG_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP0658196.1"
                     /translation="LRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLRDAG
                     QCCICGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPDPTL
                     PEVSGGSGRADDIIGL"
     gene            649..1212
                     /locus_tag="ACHLAG_005137"
     CDS             649..1212
                     /locus_tag="ACHLAG_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP0658197.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQGETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQQDETNPEMPPPVVIS
                     PSIDYMEDGLPDPVKAMGRILVENLIIDPKLALDAAWRLAQFTHYKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            1209..2870
                     /locus_tag="ACHLAG_005138"
     CDS             1209..2870
                     /locus_tag="ACHLAG_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP0658198.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPSPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQCKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRVSLEDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPLMAWCVGNARVEPR
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            2934..>3277
                     /locus_tag="ACHLAG_005139"
     CDS             2934..>3277
                     /locus_tag="ACHLAG_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658199.1"
                     /translation="MNLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLAKPVPDMPSQLAARLDEAWAAIK
                     TGLVRGLSVGFRP"
BASE COUNT          732 a          807 c         1047 g          691 t
ORIGIN      
        1 agcttcgtac actccagagc agaatcaaaa cactgaatac ccgacgggtg aatattctga
       61 agggggaaca gcgtcgtgtc agtggcagtg cacgtgtttc cctcaagcgt catatctggc
      121 tcagggacgc cgggcagtgc tgtatctgtg gtcgtgtggt tgacctctgt gacagtgaac
      181 ttgatcaccg aattgcactt cagttcggtg gtggtaatga ggagacgaat ctctggacgc
      241 tctgtaccga atgccatcga caaaagtctg ctcgtgaagc ggcgggtggt atgccggacc
      301 cgacgctgcc ggaggtgtcc ggaggtagtg gcagagcgga cgacatcatc ggactgtaac
      361 ccgacccggg ggggggatca tccggcgtaa aaaacgatcg ctccggacac cgcgccccct
      421 ctcatgcaga gaaaaaattc ccgtttcagg ccagttaaca tgttaactgg ctgttcggcc
      481 ttttttctgt ttttatcttt ataattcagt ttattgcgtg gaaaaaatgt taaccgactt
      541 tttcggaaaa tgttaaccag gcagcagtta acattcgggg tatgcgcggt gtgggaaaac
      601 ggcctgatgc atgcccggct gagagagttt tgaccccggg agggcactgt gctgacgacg
      661 caaaaacgaa aatttgcgct ggcgctcatg tccgggaaaa acaaaacagc gtcagccatt
      721 gcggcaggtt attcggcgaa gacggccaga gttaaaggct cgcagctggc aaaagatccg
      781 gaggtgcttg cgtttatagc ccgtaaacag ggcgagacgg tggaggtgga tgaggttcct
      841 gtttaccggc agaaaaaatc agagcaggag gataaacccc gtcgccgtga ggcggctgca
      901 ataccacagc aggacgaaac aaatccggag atgccaccgc ccgtggtgat atctcctagt
      961 attgattaca tggaagacgg tcttccagat ccggtaaaag caatggggag gatcctggtg
     1021 gaaaacctga taattgaccc gaaactggca ctggatgcgg cctggcgact ggcgcagttc
     1081 acacactata aaaaaggcga tgctggtaaa aaatcggcaa aaggtgatgc cgctaaaaaa
     1141 gcggctaacc gttttgcggt gccaccacca ccccgactgg tggtgaataa tgataatgag
     1201 ggcaacggat gatacctgtg tggagcacgg cctgcccgga ctgggcagag cgcctgaaaa
     1261 aggggctgtc gattattccg tctccgattt acccggagca ggccgcacat gccctggcga
     1321 tttttaaaca actgcggatt gtggatgcac cgggcagccc gacgttcggt gagtcctgtg
     1381 cacagtgggt gtttgacctg gtggcggctc tgtttggctc ctacgatgcg cagaccggtg
     1441 tccgccatat caaggaagtg ttcattctga tacccaaaaa aaacagcaag tccacactgg
     1501 ccgccgggat catgatgacg gcgctgttac tgaactggcg gcaggcggcg ggttacacga
     1561 ttctggcccc gactgtggag gtggcagcca acgccttcaa tcctgccagg gatatggtac
     1621 gacgggacga tgatctggat gacctctgcc aggtacagac ccatatccgg accatcaccc
     1681 acagggtgac agacaccacc ctgaaggtgg tggcagccga tccgaatacg gtgtccggta
     1741 tcaagtccgt gggtacgctg attgatgagt tgtggctgtt tggcaagcag tgcaaggcgg
     1801 aggacatgtt acgtgaagcc ataggcggcc ttgcctcccg cccggaaggg tttgtggtgt
     1861 atacgaccac ccagtcgaat gaaccgcccg ccggggtgtt cagacagaaa ctgcagtacg
     1921 cccgggatgt gcgcgacggc aaaattcatg atccgcactt tctgccggtg atatttgaac
     1981 accctcctga aatggtggaa agcggggctc acttgctgat ggaaaacctc gccatggtca
     2041 atccgaatct cggctattcg gtggatgagg cctttctgta ccgggagtac cgtaaagccc
     2101 gggaggctgg tgaggaagca tttcgtggct tcatgtcaaa acacgccaat gtggaaattg
     2161 gccttgccct gcgttctgac cgctgggcgg gcgcggattt ctgggagcag cagggcaggc
     2221 gcgtcagcct ggaagatatc ctgcagcgcg ctgatgtggt gacggtgggg attgacggcg
     2281 ggggcctgga tgatctgctg ggaatgtacg tgattggtcg tgatcgggag acccgcgaat
     2341 ggttaggctg gggccatgcc tgggcgcatg aaaccgcggt ggtccgacgg aagagtgagg
     2401 catcccggtt tcaggatttt gtggcctgtg gagacatgac gattgtccgt cgggtcgggg
     2461 atgacacggc ggaagtggcg gagtatgtgc gtcgtattca tgaggctgag ttactggatc
     2521 atatcggtat tgacccgtca ggtgtggggc agattctgga ttcactggcg gaagccggga
     2581 tccccgacgg aattgttgtg gggataagcc agggctggaa gctgggcggg gccatcaaaa
     2641 ccaccgaacg caaactggct gaaggggtgc tggtgcatgg tggccagcca ctgatggcct
     2701 ggtgcgttgg caatgcccgg gtggagccca gaggtaacgc catccttatt accaaacagg
     2761 ccagcggacg ggggaaaatc gacccgctga tggcgctttt caatgcggta tccctgatgt
     2821 ccctgaatcc ggagcccaaa aagaaagaat atgcggtttt tttcatataa ccctgttcac
     2881 cctgtaacca tcatggaccg ctgcggcggt ttttttattt tcaggaggct gatgtgaatc
     2941 ttaaacgggc ctgctccctg ctgacggtga aatccttcag tgaggatgag cgggtgatca
     3001 ccgggattgc gtcaacgcct tctccggatc gggatggtga catcctggag ccggaggggg
     3061 cggagttcgg cagtgcgatc ccgtttctct ggcagcatga ccattcccgc ccggtggggc
     3121 agtgtacggt gcgccgggtc agcgaagggc tggaaatcac ggcaacactg gcgaagcctg
     3181 tgccggatat gccatcgcaa ctggcagccc gactggatga ggcctgggcg gccattaaga
     3241 ccgggctggt caggggactg tccgtgggct tccgtcc
//
LOCUS       DAZGVQ010000287         3264 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000287 DAZGVQ010000000
VERSION     DAZGVQ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.290"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(68..>3264)
                     /locus_tag="ACHLAG_005140"
     CDS             complement(68..>3264)
                     /locus_tag="ACHLAG_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0658200.1"
                     /translation="ASRMGSEYYRTLQNGGRQAAAAAREQRRALAELNSQLTEIRASA
                     VGMTGAFAGAFATGHLISLADEWSSVNARLKQASQSSDEFSSSQKVLMDISQRTGTAF
                     SDNAALFARSAASMREYGYSADDVLKVTEAISTGLKISGASTAEAGSVITQFSQALAQ
                     GVLRGEEFNSVNESGDRIIRALAAGMGVARKDLKAMADDGQLTADKVVPALISQLGIL
                     RDEYAAMPETVADGITKVENAFMAWVGGANEASGATKTLSGVLNGVAGNIDTVATAAG
                     VLVAVGVARYFGNMASGVGKATTELLAAKSGTVALAAAQADAAVQVQRKAAADALAAE
                     RAWQLAQAELAVARNTSAEALVTQNAVAKRRAMIVANAALVQSNHAVAASGKALENAT
                     SVTGMLKSGAASLLSLVGGIPGLLMLGAGAWYTVYQRQEQARESARQYASTIDEIRAK
                     LPGMTLPELADNEGKTREALEEQNRLIAEQESKVRGLKTQIADYQRWLQENPQGGSGA
                     EIITHGLAEATGRLAVEQSRLTQMQERARSVQETLASLEYNRVARIREEAAEQNRAYQ
                     ALLQMNVQHTEFNRLLGLGNELLQQRQGLASVPLRLPQATLDDKQQSALNNTERQLAL
                     SRRKGEEKERARLGYAADDLGLVGDTYQEARQRYIRNSMEAWRNNEANKPKSRGGKSE
                     TEKAEDSFSRLLKQQKEQLGLAGKNTELAKLKYQTSQGELKTLTEIQKQELLRNAALI
                     DQKKIREQLRAREETLKNDNADARASNDAELLGYGQGERGRERMRELQQIRDGYRQKD
                     ADLQSQYQTGDISEDFYRQALAQNAQYLSERLKDQEAFYAESDAQRADWQKGLQEGVS
                     NWVDSASDYASQAAQLATDGISGMVSNITDMLNGNKVEWRSWAASILQEISKVLMNAA
                     IVNGIKMAANSMSGAGGFFGSIGNWLGGAVANAKGGVYTSANLSAYSNSIVDTPTYFA
                     FAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLVYSPVYHIAIQNDGANG
                     QIGPEAAGTLVQLIDQRVQAVMLSMRRDGGMLSG"
BASE COUNT          601 a         1106 c          768 g          789 t
ORIGIN      
        1 cgtcatcacc gacggtttct ccgtcacctg catcccttcc cggggacacc agtgaagggt
       61 cttaatctca tccactcagc attcccccgt cacgtcgcat ggacagcatc accgcctgta
      121 cccgctggtc aatcagttgc acaagggtgc ccgccgcttc cggccctatc tgcccgttag
      181 cgccgtcatt ctgaatggcg atgtggtaca ccggagaata caccagaccc gcactgccgt
      241 tcatgctgcc caccgcacgc acgccgagtg aaccatccgc tgcccgggtc aggggcatta
      301 tggcttcagg tccggcctcc cccatcagcc ctgccccttt tgcaaaggca aagtacgtgg
      361 gcgtgtccac aatactgtta ctgtatgcac tcaggtttgc cgaggtataa acgccgcctt
      421 ttgcatttgc caccgcgcca cccagccagt tgcctatact gccgaaaaat cctcctgcac
      481 cggacatact gtttgccgcc atcttaatgc cgttgacaat ggccgcattc ataagaactt
      541 ttgatatttc ctgcaggatt gatgcagccc agctgcgcca ttccacttta tttccgttca
      601 gcatatccgt gatgttactt accatccctg agataccgtc tgtcgcaagc tgtgctgcct
      661 gtgaagcgta atctgatgcg ctgtccaccc agttgctgac cccctcctgc agtcctttct
      721 gccagtcagc acgctgcgca tccgattcgg cataaaaagc ctcctggtct ttaaggcgtt
      781 cactcagata ctgcgcattc tgcgccagcg cctgtctgta aaaatcctca ctgatatccc
      841 ctgtctgata ctgagactgc aggtccgcat ccttctggcg gtagccgtca cgaatctgct
      901 gcagctcccg catccgttca cggccccgct caccctgccc gtaccccagc agctctgcgt
      961 catttgatgc ccttgcgtct gcattatcat ttttcagggt ctcctcccgg gcccgtaact
     1021 gctcccggat tttcttctgg tcaatcaggg cagcattgcg cagcagctcc tgcttctgta
     1081 tctccgtcag ggttttcagc tcgccctgcg aggtctggta tttcagtttt gccagttctg
     1141 tattcttccc tgccagtccc agttgttcct tctgctgctt cagcagacgg gaaaaactgt
     1201 cttccgcttt ttccgtctca gattttccgc cccgggattt gggtttattc gcctcattat
     1261 tgcgccaggc ttccatcgaa ttacggatgt aacgctgcct cgcctcctga tacgtatccc
     1321 ccaccagacc aaggtcatcc gccgcatacc ccagccgggc acgctctttt tcttcccctt
     1381 tccgccggga cagggcaagt tgacgctctg tgttattcag ggcgctctgc tgtttatcat
     1441 ccagagtggc ctgtggcagt cgcagcggta cactcgccag tccctgacgc tgttgcagca
     1501 gttcattacc cagccccagc aggcggttga attccgtatg ctgaacattc atctgcagta
     1561 atgcctggta tgccctgttc tgctctgccg cctcctcacg tatcctcgct acacggttgt
     1621 actccagtga cgccagcgtt tcctgtacag acctcgccct ttcctgcatc tgtgtcagtc
     1681 gtgactgttc aaccgccagc ctgccggttg cctcagcaag gccgtgggtt ataatctccg
     1741 cacctgaacc accctgtgga ttttcctgca gccagcgctg atagtcagca atctgtgttt
     1801 tcagtccccg gactttactt tcctgctcag caatcagacg attctgctct tccagtgctt
     1861 cgcgggtttt accctcatta tcagccagtt ccggaagggt cattcctggc agctttgccc
     1921 ggatttcatc aatcgtcgat gcatactggc gcgcggactc cctggcctgt tcctgacgct
     1981 ggtacaccgt gtaccaggca ccggccccaa gcatcagtaa accgggtatc ccgccgacca
     2041 gagaaagcag ggatgcagcg ccacttttca gcattcccgt aaccgacgtg gcattctcca
     2101 gcgccttccc cgaggccgcc actgcatgat tcgactgcac cagtgcagca ttggccacaa
     2161 tcatggcccg acgttttgcg acggcattct gtgtgaccag cgcctccgca ctggtatttc
     2221 tggccacggc gagttctgcc tgtgccagtt gccaggcacg ttctgccgcc agagcgtcag
     2281 ccgccgcctt acgttgcacc tgaaccgcag catctgcctg cgccgctgcc agggcaacag
     2341 tgccagactt cgcagcaagc agttctgtcg tggcctttcc cacaccggat gccatattgc
     2401 caaagtaccg ggcgaccccg acagcaacca gcacacccgc agctgttgcc acggtatcaa
     2461 tatttccggc cacaccgttc agcacgccgg agagcgtttt cgtcgctccg ctggcctcat
     2521 tcgcgccacc cacccaggcc ataaaggcgt tttccacctt cgtgatcccg tcagccaccg
     2581 tttccggcat ggctgcatat tcatcacgca atatccccag ctggctgatt aacgcgggaa
     2641 cgactttatc cgccgtcagt tgaccatcgt ccgccatcgc cttcagatct ttacgggcca
     2701 cgcccatgcc tgcagccagt gcgcgaatga tccggtcccc actttcattg accgaattaa
     2761 attcctcacc gcgtaacaca ccctgtgcca gcgcctggct gaactgggtg atcaccgaac
     2821 ccgcctctgc cgtactggca ccggaaattt tcagccctgt cgaaatggcc tccgtcacct
     2881 tcagcacatc atcagcactg taaccatatt cacgcatcga ggcagccgaa cgggcaaaca
     2941 gggccgcatt atccgaaaat gcggtgcctg tccgctggct gatatccatc agtactttct
     3001 gtgatgacga aaattcatct gatgactgcg acgcctgttt cagacgggca ttcacggagc
     3061 tccattcatc cgccagcgaa atcaggtgtc cggtggcaaa ggcaccggca aacgcaccgg
     3121 tcattccgac agccgaagcg cggatttccg tcaactggct gttcagctca gccagagccc
     3181 ggcgctgctc cctggctgcc gcagcagcct gacgtccgcc attctgcagg gtccggtaat
     3241 attcactgcc catacgggat gccc
//
LOCUS       DAZGVQ010000288         3251 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000288 DAZGVQ010000000
VERSION     DAZGVQ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.291"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(248..778)
                     /locus_tag="ACHLAG_005141"
     CDS             complement(248..778)
                     /locus_tag="ACHLAG_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFP0658201.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRRMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISKVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            complement(787..1146)
                     /locus_tag="ACHLAG_005142"
     CDS             complement(787..1146)
                     /locus_tag="ACHLAG_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0658202.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLVIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLIDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            complement(1159..2208)
                     /locus_tag="ACHLAG_005143"
     CDS             complement(1159..2208)
                     /locus_tag="ACHLAG_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0658203.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGHESMPVFQNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGAGSMVLCWHCDNQLRDQTSESLGQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAVCNQVADALPEAVLRRSLGLRAEKIRSVYRESDIIPGEQTATSILKQR
                     TKNIAPPSHARQQQNPPQEKTVVCITVDPESPESFMRRPKRRRWVNEKYTRWVKTQPC
                     ACCGQPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLAFEENHGSQIDL
                     IFRFLDHAFATGVLG"
     gene            complement(2210..2488)
                     /locus_tag="ACHLAG_005144"
     CDS             complement(2210..2488)
                     /locus_tag="ACHLAG_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658204.1"
                     /translation="MAHDTKSHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGYEFQKDLSLDRLHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(2656..2811)
                     /gene="hokD"
                     /locus_tag="ACHLAG_005145"
     CDS             complement(2656..2811)
                     /gene="hokD"
                     /locus_tag="ACHLAG_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP0658205.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TVYEPEE"
BASE COUNT          755 a          905 c          757 g          834 t
ORIGIN      
        1 agcatgtctt tttccctttt ccggttaacg tgacacacca ataactcttg tcgaaaaagc
       61 cagcaacctg aaagaacggt attaatgacc accagcgaat ttattgcgct gctgtatatt
      121 acggacacaa aaaaaaccac cttccggtgg cttccttgtg cgaaaaaact tgcatttcgc
      181 ctcgcgatac agctttgcga agcttacagg aattcaagct gtttctgcgt aaaaaagcaa
      241 gcttttttta tcgaaatgaa tcgtgcatag gtacataaag catgtgttca gccacggcta
      301 accaacctgc aatacgtttc tcacatgtgc tgaaacacca ttccgggtga gtacgattta
      361 aacattctgc catttttctc ttactcattc cccgtccttc gtacctttgc cggagaatat
      421 tgattagccc gggatattcc ccaagcacct tactgataac gcgatcaata atcaacgcct
      481 ctgtgtctgt acaatgtgac aaccagctct tctgctttcc tcttgtcata tcccgaaaaa
      541 atgcctcaag ttccggtttt tccagcccgg atttcttcat tctgcgcaaa acctcattaa
      601 ctgctgtttt cgtcagcttt ttcgaaacca gtaaccggtt aaacatattt ccggatttac
      661 ccccaccgat atacgaccac cgcccccaca tccgtaattt cccctggatc cagactgctt
      721 ccagcgtgtt caggcgtaaa tgttcgccgc ttttgcctgt aatttccgga tatatcatat
      781 ttacgctcac tcactctcaa ttttgtaaat cttcacaccc agccgtccac cagatactgg
      841 ctgaccacgt acaatattga tttcatcaaa ctgctcgtca tcaatgagca ctcccgcatg
      901 cgtcagcgca tccagcggtg ctttcagaat gttgtccagg tcgcgacgac gcttatccgg
      961 tggctctgca atcaccttta tcaccagcct tccggacagg cttaatttca gccgctgctg
     1021 gcgaacaata agcgccacag cccggcgata acgctttccc tcctccgaga taaaatatgt
     1081 gctgccacgg cgtcgccagt aagtgttcac cgtcggcggg taaggtaaaa ccaaatctat
     1141 gagcatcagt cacctctttt acccaagcac gccagttgca aaggcgtgat caagaaaacg
     1201 aaaaattaaa tcaatctgag aaccatgatt ttcttcgaac gccagcggat ccgcatgaag
     1261 ttcgttgtga tgttcccggc acaacggtag cgtgaaaata tcgtgggcct ttgttcccat
     1321 ccctccctga ccgtgaccaa tcaggtgatg gggatcgtcg gctggctgac cacaacacgc
     1381 acacggctgt gtcttcaccc agcgcgtata tttctcattt acccaacggc gacgtttagg
     1441 tcgcctcatg aaagattccg gagactccgg atcaacggtg atgcatacca ccgtcttttc
     1501 ctgtggtggg ttctgttgct ggcgggcgtg agacggcggc gcaatatttt ttgtgcgctg
     1561 tttcagtatg ctggtggcgg tctgctctcc cggtatgatg tcgctttcgc gatacaccga
     1621 gcggattttt tccgcacgta accccagaga acgacgtaat actgcctccg gaagcgcgtc
     1681 cgccacctga ttgcagaccg cccaccagga taattcagcc agcgacaatt cccgctcctg
     1741 cgtgccattc attgcatggc gtatgacgtc aatcatccat gctgacaggt tttgatgagc
     1801 aagttgcccg agtgattcgg atgtctggtc acgcagctgg ttgtcacagt gccagcacaa
     1861 caccatcgag ccagcgccat aacggtgaat gacggtttca ctgtggtgat aatcgccgtg
     1921 tggccactgg caggatttaa catggcgcag taaccagtca gacaatgcgc cagcgccacc
     1981 agcagcacga atcactcgtt cgtcgctgaa aaatggcagt aatgatttat cctccgccag
     2041 cggctggcga acggcaggaa cgaccccgga cggcagatta cgcatgcttt tcggttccgg
     2101 ctccaccagt acccgggtat tttggaatac cggcatggat tcatggcccg gcttaacgat
     2161 caccagcccg agttctggca caagaacagg tcgaagtaat acccgcacgt tacctccaga
     2221 tacgttgctg gaatgtgcgg gatggacgcg gtgggcgctc ggagtaaggg agcctcacag
     2281 agattatcca gtgcagtcgg tcgaggctga ggtctttctg aaactcgtat ccgcgcctgc
     2341 ggtagcactg aattaaccat tcagcttgtt cttcagtaca tgggggatgc tggtaccagt
     2401 cagatttaaa tgcgtgagag cgccgcccgt gcctgctggc aaagacggct gaattatcag
     2461 aattgtgtga tttggtatcg tgcgccatcg gttgtctctg ctggcgcagc aggtgccagt
     2521 tgttcaggct gacgtgcgaa ttgtaaacca gaatgccagg aaaaaacaaa acccgacgaa
     2581 gcgggttaag cgcgggtgcg ttgaggatgc ctgacacatc agaggtggcg ggagatttct
     2641 cccccgcctg gtctcttact cctcaggttc gtagactgtg aagacagcga cctccgtctg
     2701 gccggttcgg attcgtacct cgcagaggtc tttcctcgtt accagtgccg tcactatgac
     2761 ggttaaacag atgacgataa gggcgattag catcgccttt tgctgcttca tagcctgctt
     2821 ctccttgcct ttcggcacgt aagaggctaa cctacgtgtg tagagcatag atatggcatc
     2881 agattaatgt taagcgtctt gccggacgcg caatgttaac tggggctttt ctctatctgc
     2941 cttttggtat tcatgcctga gacagatagc ctcaagcacc cgcagcaatt ctacttaact
     3001 atcctttccc cgcaaatcgt ttttatcccc agcgacaaat cgaatacacc accagcgcca
     3061 ccgccattgc gatccctacc gttgtgaatg cctcaggcca ggtcatcgta aaacatcctc
     3121 tgcgcttatc agtccgtttc gcttcaggta gtccatcgcc ttacccggca atttacagtc
     3181 cggcttcgtt ttcctcagtt gccaggttaa ctgctttacc agcatggtta actcgtcgac
     3241 cagacgctga t
//
LOCUS       DAZGVQ010000289         3219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000289 DAZGVQ010000000
VERSION     DAZGVQ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.292"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(89..616)
                     /gene="mobB"
                     /locus_tag="ACHLAG_005146"
     CDS             complement(89..616)
                     /gene="mobB"
                     /locus_tag="ACHLAG_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFP0658206.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(598..1182)
                     /gene="mobA"
                     /locus_tag="ACHLAG_005147"
     CDS             complement(598..1182)
                     /gene="mobA"
                     /locus_tag="ACHLAG_005147"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFP0658207.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     AQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQVGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1252..1521
                     /locus_tag="ACHLAG_005148"
     CDS             1252..1521
                     /locus_tag="ACHLAG_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFP0658208.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1598..2584
                     /gene="srkA"
                     /locus_tag="ACHLAG_005149"
     CDS             1598..2584
                     /gene="srkA"
                     /locus_tag="ACHLAG_005149"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFP0658209.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQWMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2601..>3219
                     /gene="dsbA"
                     /locus_tag="ACHLAG_005150"
     CDS             2601..>3219
                     /gene="dsbA"
                     /locus_tag="ACHLAG_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFP0658210.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSE"
BASE COUNT          808 a          777 c          787 g          847 t
ORIGIN      
        1 aggccatccg tcaggatggc ctttttgcat tggcgcagaa aaaaatgccg gatgcgacgc
       61 tggcgcgtct tatccagcct actcttgctt atccgttctg cttttgcatc cactccacca
      121 caaaatcagc cagcccctca acatcattaa tatccagtaa tgcgacatca agattaagcg
      181 gcacatcact ggctacagca ataacatgcc tgtctatcac taattcttca ggtcgatgtc
      241 cggctccatc gcgaaacagc acaatctttg cgatctcttc atgcttaaac ccttcgacca
      301 gaatcaaatc cagctttgag gtatccattc gacttgcgag aaactgtaga tccagctctt
      361 cttcgtctgg tgtttccgtc atcaaggccc atcgttgctg gctggcaacg atggtttgtg
      421 ccgcgccagc cttgcgcagc tcatagctat ctttgcctgg cttatcaaca tccatatcat
      481 ggtgcgtatg cttaatcagc cctggacgga tccctctggc gcataatgcc gggatcagtt
      541 gtttcaaaag cgtagttttt ccggtaccac tccacgcggc aaaggcgagt aacggtatca
      601 tcgtttttcc tgccatcggg caagctcctc tggcgtattc acgttaataa atgcatcttt
      661 attatcgctg aaatcaaccg catgaccgcc agccagacgc ataaatgcca ttacccggcg
      721 ttctcctact tgcagatatt ccagtaataa aggctcaatt gcgcggttta ccagagcaat
      781 agtcgggtga tcgcgttcac cgtcgtggac ccacacgaca ggcgcatctt tgcgctgatg
      841 attaagccgg gctgctaaat cctcgggaat ataaggagta tcgcacggac aaaacaaaaa
      901 ccactcgcct gcttcctgct gcattactga aagcattcct gccagagggc ctgggtaatc
      961 cgccagtgaa tcttcaatca ctttcagacc gcttgcctga tagatttcct gatgacgatt
     1021 agcattaacc acgacgtgag agagctgcgc cataagcgcg tcagcgacat gttgccataa
     1081 tggtttgccg tttaattcca gcaatccttt atctacgccg cccattcgtc tggctttacc
     1141 gcctgccagc acaacgcctg ttatcgtcgt catcagattc accgatatcg cctcttttat
     1201 tgtgggattg accctgctaa cgtgtctgtc tcaagagtaa ggagcattac tatgaaatgt
     1261 aaacgtctga atgaagttat tgaactcctc cagccagcct ggcaaaaaga gccagacctt
     1321 aacctgttgc aatttttgca gaaactggcg aaagagtcag gttttgacgg cgaactggcg
     1381 gatttgacgg atgacattct gatctatcac ctgaaaatgc gtgattccgc gaaagatgcg
     1441 gtgatcccgg gtttgcagaa agattatgaa gaagacttca aaacggcgct gttacgcgct
     1501 cgcggcgtaa ttaaagagta aaagcttgta agcggtgcca ccaaaatcat cgtgaaatga
     1561 tatccttcat cattcgtaat gttttccgga tgatgggatg aacaacagcg cttttacttt
     1621 ccagacacta cacccggata ccatcatgga tgctctattt gagcaaggga tccgggtgga
     1681 ttccggtctt accccgctta acagctatga aaaccgtgtc tatcaatttc aggacgaaga
     1741 tcgtcgacgt tttgtcgtca aattttatcg ccctgaacgt tggacagccg atcaaatcct
     1801 cgaagaacat caatttgcgt tgcagttggt aaatgatgaa gttccggtcg cagcacctgt
     1861 ggcctttaac ggtcagactt tattgaatca tcagggattt tatttcgctg tttttccaag
     1921 cgtcggtggt cgccagttcg aagctgataa tatcgatcag atggaagcgg ttgggcgtta
     1981 tttagggcgt atgcaccaga cggggcgcaa acagcttttt atccatcgcc cgaccatcgg
     2041 tctgaatgaa tatctcattg agcctcgcaa gctgtttgag gacgctacac tgataccttc
     2101 cgggttgaaa gcggcattcc tgaaagcgac agatgagctg atcgccgccg ttacagcaca
     2161 ctggcgggaa gacttcaccg ttctgcggct acatggagac tgccacgccg ggaatattct
     2221 ctggcgcgat ggtccaatgt ttgttgatct ggatgatgca cgtaatggtc cagctattca
     2281 ggatttgtgg atgttgctca atggcgataa agccgagcag tggatgcaac tggaaactat
     2341 tattgaagct tatgaagaat ttagcgagtt cgacaccgct gaaatcggac tgattgaacc
     2401 tttacgcgcc atgcgtttgg tttattatct tgcctggcta atgcggcgtt gggctgatcc
     2461 cgcgttcccg aaaaattttc cgtggttaac cggggaagat tactggctgc gacagacggc
     2521 gacttttata gaacaggcaa aagttctaca agaaccccct ttgcaattaa cacctatgta
     2581 ttaatcggag agagtagatc atgaaaaaga tttggctggc gctggctggt ttagttttag
     2641 cgtttagcgc atcggcggcg cagtatgaag atggtaaaca gtacactacc ctggaaaaac
     2701 cggtagctgg cgcgccgcaa gtgctggagt ttttctcttt cttctgcccg cactgctatc
     2761 agtttgaaga agttctgcat atttctgata atgtgaagaa aaaactgccg gaaggcgtga
     2821 agatgactaa ataccacgtc aacttcatgg gtggtgacct gggcaaagat ctgactcagg
     2881 catgggctgt ggcgatggcg ctgggcgtgg aagacaaagt gactgttccg ctgtttgaag
     2941 gcgtacagaa aacccagacc attcgttcag catctgatat ccgcgatgta tttatcaacg
     3001 caggtattaa aggtgaagag tacgacgcgg cgtggaacag cttcgtggtg aaatctctgg
     3061 tcgctcagca ggaaaaagct gcagctgacg tgcaattgcg tggtgttccg gcgatgtttg
     3121 ttaacggtaa atatcagctg aatccgcagg gcatggatac cagcaatatg gatgtttttg
     3181 ttcagcagta tgctgataca gtgaaatatc tgtccgaga
//
LOCUS       DAZGVQ010000290         3208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000290 DAZGVQ010000000
VERSION     DAZGVQ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.293"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            161..1843
                     /gene="ydeN"
                     /locus_tag="ACHLAG_005151"
     CDS             161..1843
                     /gene="ydeN"
                     /locus_tag="ACHLAG_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP0658211.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1895..3052
                     /gene="ydeM"
                     /locus_tag="ACHLAG_005152"
     CDS             1895..3052
                     /gene="ydeM"
                     /locus_tag="ACHLAG_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP0658212.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQCYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCLKCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3018..3140)
                     /locus_tag="ACHLAG_005153"
     CDS             complement(3018..3140)
                     /locus_tag="ACHLAG_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658213.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT          972 a          690 c          642 g          904 t
ORIGIN      
        1 acttaataga acagaaagta atgtcaacgc tttatggact gttatttccc tttttttagc
       61 taaatctgct atctctttat gtgactaact tcacttacat ccacttattt ctcttcgtaa
      121 aattacttcg gaattaagta caataagaag aggaacattt atgaagtctg cattaaagaa
      181 aagtgtcgta agtacctcga tatctttgat actggcatct ggtatggctg catttgctgc
      241 tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca aacgttgctt tctcagactt
      301 tacgccgact gaatacagta ccaaaggaaa gccaaatatt atcgtactaa ccatggatga
      361 tcttggttat ggacaacttc cttttgataa gggatctttt gacccaaaaa caatggaaaa
      421 tcgtgaagtt gtcgatacct acaaaatagg gatagataaa gccattgaag ctgcacaaaa
      481 atcaacgccg acgctccttt cattaatgga tgaaggcgta cggtttacta acggctatgt
      541 ggcacacggt gtttccggcc cctcccgtgc cgcaataatg accggtcgag ctcccgcccg
      601 ctttggtgtc tattccaata ccgatgctca ggatggtatt ccgctaacag aaactttctt
      661 gcctgaatta ttccagaatc atggttatta cactgcagca gtaggtaaat ggcacttgtc
      721 aaaaatcagt aatgtgccgg taccggaaga taaacaaacg cgtgactatc atgacaactt
      781 caccacattt tctgcggaag aatggcaacc tcaaaaccgt ggctttgatt actttatggg
      841 attccacgct gcaggaacgg catattacaa ctccccttca ctgttcaaaa atcgtgaacg
      901 tgtccccgca aaaggttata tcagcgatca gttaaccgat gaggcaattg gcgttgttga
      961 tcgtgccaaa acacttgacc agccttttat gctttacctg gcttataatg ctccgcacct
     1021 gccaaatgat aatcctgcac cggatcaata tcagaagcaa tttaataccg gtagtcaaac
     1081 agcagataac tactacgctt ccgtttattc tgttgatcag ggtgtaaaac gcattctcga
     1141 acaactgaag aaaaacggac agtatgacaa tacaattatt ctctttacct ccgataatgg
     1201 tgcggttatc gatggtcctc tgccgctgaa cggggcgcaa aaaggctata agagccagac
     1261 ctatcctggc ggtactcaca ccccaatgtt tatgtggtgg aaaggaaaac ttcaacccgg
     1321 taattatgac aagctgattt ccgcaatgga tttctacccg acagctcttg atgcagccga
     1381 tatcagcatt ccaaaagacc ttaagctgga tggcgtttcc ttgctgccct ggttgcaaga
     1441 taagaaacaa ggcgagccac ataaaaatct gacctggata acctcttatt ctcactggtt
     1501 tgacgaggaa aatattccat tctgggataa ttaccacaaa tttgttcgcc atcagtcaga
     1561 cgattacccg cataacccca acactgagga cttaagccaa ttctcttata cggtgagaaa
     1621 taacgattat tcgcttgtgt atacagtaga aaacaatcag ttaggtctct acaaactgac
     1681 ggatctacag caaaaagata accttgccgc cgccaatccg caggtcgtta aagagatgca
     1741 aggcgtggta agagagttta tcgacagcag ccagccaccg cttagcgaag taaatcagga
     1801 gaagtttaac aatatcaaga aagcactaag cgaagcgaaa taactaaacc ttcatgcggc
     1861 ggaattttcc gccgccttat tgagcgagat agcgatgcac gttacagcca agccctccag
     1921 ttttcaatgt aatctcaaat gtgattactg tttttacctt gaaaaagagt cgcagtttac
     1981 tcatgaaaaa tggatggatg acagcactct gaaagagttc atcaaacaat atatcgcagc
     2041 gtctggcaat caggtctatt ttacctggca aggcggtgaa cccactctgg ctggcctgga
     2101 ttttttccgt aaagttattc actatcaaca atgctatgca ggccaaaaac gtatttttaa
     2161 tgcattacaa acgaatggca ttttattgaa taatgaatgg tgtgcttttc tcaaagaaca
     2221 tgagtttctg gttggtatct cgatcgatgg cccccaggag ctacatgacc gttacagacg
     2281 cagtaattca ggtaacggta cttttgcaaa agtgatagca gccatcgagc gtctgaaatc
     2341 atatcaagtt gagtttaata cgttaaccgt cattaataac gttaatgtcc attaccctct
     2401 tgaggtttat cattttttaa aatctatcgg cagtaaacat atgcaattta tcgaattgct
     2461 cgaaaccggg acgccgaata ttgatttcag tggtcatagt gagaacacat tccgtatcat
     2521 tgatttttct gtgcctccca cggcttatgg caagtttatg tcaaccattt ttatgcaatg
     2581 ggttaaaaac gatgtgggtg aaattttcat ccgtcagttt gaaagctttg tcagccgttt
     2641 tttagggaat gggcatacca gttgtatttt ccaggagtcc tgcaaggata atctggttgt
     2701 tgaaagtaat ggagatattt atgaatgcga ccattttgtc tatccacagt acaaaattgg
     2761 aaacattaat aaatctgaac tcaaaacgat gaacagtgta caactgacag cgcaaaaaaa
     2821 acggatttca gcgaaatgtc tgaaatgtgc atataaacct atctgcaatg gcggttgtcc
     2881 taagcatcgt attactaaag taaacaatga gactgtttct tatttttgcg aaggttataa
     2941 aatccttttt tcaaccatgg taccttatat gaacgccatg gtggagttag ctaagaacag
     3001 agtaccgctt taccacatta tggatgttgc aaaacaaatg gagaataatt aataacgctc
     3061 tcttttaact ccattcttag cctccttctt atgaccttaa caaggcaccc gacataaatt
     3121 cctgtcgggt gtttcgtcat catcatcttc ccttgcgaaa atcctggctt acgtggtatg
     3181 cgtcagggca ttagcaatac ccttgatt
//
LOCUS       DAZGVQ010000291         3196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000291 DAZGVQ010000000
VERSION     DAZGVQ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.294"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            13..687
                     /gene="yehC"
                     /locus_tag="ACHLAG_005154"
     CDS             13..687
                     /gene="yehC"
                     /locus_tag="ACHLAG_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0658214.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDTPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            703..3183
                     /gene="yehB"
                     /locus_tag="ACHLAG_005155"
     CDS             703..3183
                     /gene="yehB"
                     /locus_tag="ACHLAG_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP0658215.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQTYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
BASE COUNT          916 a          600 c          815 g          865 t
ORIGIN      
        1 agaggcatta agatgaaagg attattatct ttactcattt tttctatggt ccttcctgca
       61 catgccggaa ttgttatcta cgggacgcgc attatttacc cggcagaaaa taaagaagtt
      121 atggtgcagt tgatgaacca gggaaaccgt tcttcgctgc tgcaggcgtg gattgatgat
      181 ggcgatacgt cgttaccacc agaaaaaatt caggttcctt tcatgttaac gccaccagtg
      241 gcaaaaatag gggcaaattc cgggcagcaa gtaaaaatca aaattatgcc gaataaactg
      301 cccactaata aagaaagcat tttttatctg aatgttctgg atactccacc aaatagtcca
      361 gagcaagaag gcaaaaatgc actgaagttt gcgatgcaaa acagaattaa gttgttttac
      421 cggccagcgg gtattgctcc ggtaaataaa gcgaccttta aaaagttact ggtaaatcgc
      481 agtggcaatg gattggtgat aaaaaatgac tcagctaatt gggtgacgat ttcggatgtc
      541 aaagctaata atgttaaagt caattatgaa actataatga ttgccccctt agaaagtcag
      601 agtgttaatg tcaaaagtaa taatgcaaat aactggtatc tgaccattat cgatgaccac
      661 ggcaactata ttagtgacaa aatttaattg caggagctgc ctatgttgag aatgacccca
      721 cttgcatcag caatcgtagc gttattgctc ggcattgaag cttatgcagc tgaagaaacc
      781 tttgataccc attttatgat aggtggaatg aaagaccagc aggttgcaaa tattcgtctt
      841 gatgataatc aacccttacc ggggcagtat gacattgata tttatgtcaa taagcaatgg
      901 cgcgggaaat atgagattat tgttaaagac aacccgcaag aaacatgttt atcaagagaa
      961 gttatcaagc ggttaggcat taatagcgat aacttcgcca gcggtaagca atgtttaaca
     1021 tttgagcaac ttgttcaggg tgggagctat acctgggata tcggggtttt tcgtctcgat
     1081 ttcagtgtcc cgcaggcctg ggtggaagaa ctggaaagtg gctatgttcc accggaaaac
     1141 tgggagcggg gtattaatgc gttttatacc tcttattatg tgagtcagta ttacagtgac
     1201 tataaagcgt cgggtaatag taagagtaca tatgtacgtt ttaacagcgg gttaaattta
     1261 ctggggtggc aactgcattc tgatgccagt ttcagtaaaa caaataacaa tccaggggtg
     1321 tggaaaagca ataccctgta tctggaacgt ggatttgccc aacttctcgg cacgcttcgc
     1381 gtgggtgata tgtacacatc aagcgatatt tttgattctg ttcgcttcag aggtgtgcgg
     1441 ttgtttcgtg atatgcagat gctgcctaac tcgaaacaaa attttacgcc acgggtgcag
     1501 gggattgctc agagtaacgc gctggtaact attgaacaga atggttttgt ggtttatcag
     1561 aaagaggttc ctcctggccc gtttgcgatt acagatttgc agttggccgg tggtggagca
     1621 gatcttgatg tcagcgtgaa agaggcggac ggttcggtaa ccacctatct ggtgccttat
     1681 gcagcggtgc caaatatgct gcaacccggc gtgtcgaaat atgattttgc ggcgggtcgt
     1741 agccatattg aaggggcgag caaacaaagt gattttgtcc aggcgggtta tcagtatggt
     1801 tttaataatt tattgacgct gtatggtggc tcgatggtcg cgaataatta ttacgcgttt
     1861 actttgggga ctggctggaa tacacgcatt ggtgccattt ccgtcgatgc cacgaagtcg
     1921 catagtaaac aagacaacgg cgatgtgttt gacgggcaaa gttatcaaat tgcctacaac
     1981 aaatttgtga gccaaacgtc gacgcgtttt ggtctggcgg cctggcgtta ttcatcgcgt
     2041 gattaccgga catttaatga tcacgtttgg gcaaacaata aagataatta tcgccgtgat
     2101 gaaaacgatg tctatgacat tgccgattat taccagaacg attttggccg taaaaatagc
     2161 ttctctgcca atatgagcca gtcattgcca gaaggctggg ggtctgtgtc cttaagtacg
     2221 ttatggcgag attactgggg gcgtagcggc agcagtaagg attatcagtt gagttattcc
     2281 aataactggc ggcggataag ctatatcctc gcggcaagcc agacgtatga cgagaatcat
     2341 catgaagaga aacgttttaa tatttttata tcgattccct ttgattgggg tgatgacgtt
     2401 acgacgcctc gtcggcaaat atatatgtct aactcaacga cgtttgatga tcaggggttt
     2461 gcctcaaata atacgggatt atcaggaacc gttggaagcc gggatcagtt taactatggg
     2521 gtcaacctga gttatcagaa tcagggaaat gaaacgacag ctggggcgaa tttgacctgg
     2581 aacgcgccgg ttgcgacagt gaatggcagt tatagtcagt cgagtactta tcgacaggct
     2641 ggagccagtg tttcaggggg cattgtcgcc tggtcggggg gcgttaatct ggcgaaccgt
     2701 ctttccgaaa cgtttgctgt gatgaatgcg ccaggaatta aagatgctta tgtcaatggg
     2761 caaaaatatc gcacaacaaa ccgtaatgga gtggtggtat acgacggaat gacaccttat
     2821 cgggaaaatc acctgatgct ggatgtgtcg caaagcgata gcgaagcaga attacgtggc
     2881 aaccggaaaa ttgccgcacc ttatcgcggc gcggttgtac tggttaattt tgataccgat
     2941 cagcgcaagc cctggtttat aaaagcgtta agagcggatg ggcaaccatt aacgtttggt
     3001 tatgaagtca atgatatcca cggtcataat attggcgttg tcggccaggg aagccagtta
     3061 tttattcgca ccaatgaaat accgccatcg gttaatgtag caattgataa gcaacaagga
     3121 ctttcatgca caatcacctt cggtaaagag attgatgaaa gtagaaacta tatttgccag
     3181 taatagtcag gtggtt
//
LOCUS       DAZGVQ010000292         3016 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000292 DAZGVQ010000000
VERSION     DAZGVQ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.295"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1752
                     /locus_tag="ACHLAG_005156"
     CDS             <1..1752
                     /locus_tag="ACHLAG_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0658216.1"
                     /translation="GQTPSWYYVVVIAGQSNASSFGEGLPLPDTYDRPDPRIKQLARR
                     NTVTPGGVECAYNDIIPADHCLHDVLDMSNHNHPKADLSKGQYGCVGQGLHIAKKLLP
                     FIPEEAGILLVPCARGGSAFTDGADGEFTEASGATSASSRWGVNKPLFSDLVNRTKAA
                     LSSNPRNILLSVVWMQGENDLKTGKHAEHSGLFVTMVETFRRELSAFSAQSPAGDMAS
                     VPWICGDTTYYWKNTYPEQYKAVYGKYANEAKNIHFVPFVVDENGKNVPTNNPDEDPD
                     IPGIGYYGAISRDSTNWTSNDRASHFSSWARRGIISDRLATAILTLSGQKSAFFRGVT
                     ATEHSDTGKSEPEPTGDVKDVSDQMKIIVSYTANAPTEPMKDQGWNAASGKATFVTDA
                     AATGGNKALRVNKAPNSMASWKMYHPITADNARDLFARGGDISLRFKIPSDAGIAENR
                     YGVGIYWAVSNWPDGDGKNNLIASCFLQTDSANLNVMYHKGSGSQQIGSYGVFDHEWH
                     TLSLRFAGGNSMSVTPVLDGKDGEPFELVKWVNAGFTVDQLTLTDITGNAATYPMLFD
                     TIKVQVNDAVSPSNVNK"
     gene            1788..1967
                     /locus_tag="ACHLAG_005157"
     CDS             1788..1967
                     /locus_tag="ACHLAG_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0658217.1"
                     /translation="MPILHTFMLYFCTCVCLLYLLSGGYKIIRNYFRNKIDAAAEEKI
                     KSGAVMPQPKDSGQI"
     gene            2003..2248
                     /locus_tag="ACHLAG_005158"
     CDS             2003..2248
                     /locus_tag="ACHLAG_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP0658218.1"
                     /translation="MPEIKGTVTEELVKQALYSEEVNRVLKAQVRKDFEAQIDAYVDE
                     VLARMVGHSPAENSTENEPQPVEQPEPVQPGTDSAMM"
     gene            2323..2523
                     /locus_tag="ACHLAG_005159"
     CDS             2323..2523
                     /locus_tag="ACHLAG_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658219.1"
                     /translation="MDKNKKQPDTKGLEILSAEIINALRSGLDENTLQDRNFTTAMWI
                     AMQILCTPRCAIFAVREGQLTR"
     gene            complement(2710..2820)
                     /locus_tag="ACHLAG_005160"
                     /pseudo
     CDS             complement(2710..2820)
                     /locus_tag="ACHLAG_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2843..3016)
                     /locus_tag="ACHLAG_005161"
                     /pseudo
     CDS             complement(2843..3016)
                     /locus_tag="ACHLAG_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          851 a          636 c          734 g          795 t
ORIGIN      
        1 ggtcaaactc cgtcctggta ttacgtggtt gttattgctg gtcagtcgaa tgcatcgtca
       61 tttggtgaag gtcttccttt gcctgatacc tatgatcgac ctgatccacg tattaaacag
      121 ctggctcgcc gtaacacggt tacacctggc ggggttgaat gcgcctacaa cgatataatt
      181 ccggcagatc actgtcttca tgatgtgctg gatatgagta accataatca tccaaaagca
      241 gatctttcaa aaggtcagta tggttgtgtc ggccagggtt tgcatattgc aaaaaaactc
      301 ctgcccttta tcccggaaga ggctggtatc cttctggttc cctgtgccag aggaggctct
      361 gcatttaccg atggtgctga tggtgaattt actgaagctt ccggagcaac atctgcatca
      421 tcgcgctggg gagtcaataa acctttgttc agcgatcttg ttaaccgaac aaaagcggct
      481 ctcagcagta accccagaaa tattctcctg tccgttgtct ggatgcaggg cgagaatgat
      541 ctgaaaacag gaaaacatgc tgaacattcc ggtctgtttg tgacgatggt cgaaacattc
      601 aggcgtgaac tttcggcttt ttctgcgcaa tctccagccg gagatatggc ttctgtaccg
      661 tggatctgtg gtgatacgac gtattactgg aaaaatacat atccagaaca atacaaagct
      721 gtttatggca aatatgctaa tgaagcaaag aacattcact ttgttccatt tgttgtggat
      781 gaaaatggaa aaaatgtacc gacaaataat ccagatgaag acccggatat tccaggtatt
      841 ggctattatg gtgccataag tcgcgattcg actaactgga catcaaacga cagggcaagc
      901 catttcagtt catgggcgcg ccgtggaatt atttcagacc gtctggcaac agcgattctg
      961 acattgtcag gacaaaaatc tgctttcttc cgcggtgtaa cggcaacaga acattctgat
     1021 accggtaaga gtgagcctga acccaccggg gatgtgaagg acgtgagcga tcagatgaag
     1081 atcatcgttt cgtatactgc aaacgccccg acagaaccaa tgaaagatca ggggtggaat
     1141 gcggcttccg gaaaagcaac ctttgtcacg gatgctgctg caacaggtgg taacaaagcg
     1201 ttaagggtga ataaagcgcc taacagcatg gcctcatgga aaatgtatca tccgatcaca
     1261 gctgataatg ccagggattt gttcgccaga ggtggtgata tttccctccg tttcaaaatt
     1321 ccatcggatg cgggcattgc ggaaaaccgg tatggtgttg gtatttactg ggctgtaagt
     1381 aactggcctg acggtgacgg aaaaaataac ctcatcgcat catgtttcct gcaaacggat
     1441 tcagctaacc tgaatgtgat gtatcacaaa gggagcggaa gtcaacagat aggatcgtat
     1501 ggtgtctttg atcatgaatg gcatacgctc tcgttacggt ttgcaggtgg taatagcatg
     1561 agtgttaccc cagttctgga cgggaaagat ggtgaaccat ttgaacttgt gaagtgggtg
     1621 aatgccgggt tcacggtgga tcaactgaca ctgaccgata ttaccggcaa tgcagccact
     1681 tacccgatgt tgtttgacac aattaaagtg caggttaacg atgcggtctc tccgtctaat
     1741 gtgaataaat gacgggtaat tcattttatt taaaatggtg agtgattatg ccaatactac
     1801 atacatttat gctttatttt tgcacgtgtg tgtgtctact ttatctcctc agcggaggtt
     1861 acaaaattat ccgaaattat tttcgaaata aaattgatgc agccgcagag gaaaagataa
     1921 aaagcggtgc tgtaatgccg cagccgaaag actccggtca gatttaattt tttgtgaacc
     1981 agaaaaggaa aggagaatat tcatgccaga gattaaaggc acggttactg aagagcttgt
     2041 caaacaggca ctttattctg aagaagtgaa ccgcgtgctg aaggcgcagg ttcgcaagga
     2101 ttttgaggca caaatcgacg catatgttga tgaagtgctg gcccgaatgg ttggccattc
     2161 tccggctgaa aacagcacgg agaatgagcc tcagcccgta gagcaaccag agccggttca
     2221 gcccggaact gacagtgcca tgatgtaaca atacaaaccg acagcttctc ctgtcggttt
     2281 gattttcatg ggcgatgtat ataccagtca gaggaaataa tcatggacaa gaacaaaaag
     2341 cagcccgata ccaaaggtct ggaaatatta agcgcagaaa ttatcaatgc gctacgttca
     2401 ggacttgatg aaaatacatt acaggatcga aacttcacca ctgcaatgtg gatagcgatg
     2461 cagatcctgt gtaccccaag atgcgctatt tttgcggttc gtgagggtca gttgactcgc
     2521 tgattgcccg aattacatca gaaaagcacg cgttccataa ttgcatagct gatgaatcac
     2581 cagctgtatg aaaagaggta aagacacaac tggtggatgt cattgctgca attgtgatat
     2641 cagctgcaat tcgcgcagcc tgtaactttt ggtcggtggt cagtttatcc attttctcct
     2701 gcctgaacag atgcatctct gctttacagg aggccgggga atatcatagg atacaccacg
     2761 agctcagagt gaagcattga tgcagttttt tacagattga cccagaatag ttcgcgctat
     2821 ctgagtcgcc atttatgtag tcccgaagta aagcattgat gcaaacttgt ctaaatcggc
     2881 ccagcatagt tccagctatg ctgggtttcc cttttatgta gtatcctgtt gatttgaaac
     2941 atactacata tacgcgcgat cctaccttca ctggataaaa aggcaataaa atgatcattt
     3001 tatgatcgtt ttcacc
//
LOCUS       DAZGVQ010000293         2949 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000293 DAZGVQ010000000
VERSION     DAZGVQ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.296"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            60..1271
                     /gene="bluF"
                     /locus_tag="ACHLAG_005162"
     CDS             60..1271
                     /gene="bluF"
                     /locus_tag="ACHLAG_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFP0658220.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            1476..2207
                     /gene="bluR"
                     /locus_tag="ACHLAG_005163"
     CDS             1476..2207
                     /gene="bluR"
                     /locus_tag="ACHLAG_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFP0658221.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2428..2832
                     /gene="ycgX"
                     /locus_tag="ACHLAG_005164"
     CDS             2428..2832
                     /gene="ycgX"
                     /locus_tag="ACHLAG_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFP0658222.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            2885..>2949
                     /locus_tag="ACHLAG_005165"
     CDS             2885..>2949
                     /locus_tag="ACHLAG_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658223.1"
                     /translation="MGIKVQRLSLWMINTVLLLLP"
BASE COUNT          866 a          626 c          636 g          821 t
ORIGIN      
        1 acaagaagat atgtctgtta ccgtcttact ctcgcctcac ccattaccct ggattgttaa
       61 tgcttaccac ccttatttat cgtagccata tacgtgacga cgaacctgtc aaaaaaatcg
      121 aagaaatggt ttcgatagca aatcgcagga acatgcagtc tgacgtaaca gggatcttac
      181 tgtttaatgg ttctcatttt ttccagcttc tggaaggtcc ggaagaacag gttaaaatga
      241 tatatcgggc tatatgccag gatccacggc actataatat tgttgagctg ctgtgcgatt
      301 acgcgcctgc tcgccgtttt ggcaaagcgg gaatggaatt atttgatttg cgcctgcacg
      361 agcgagatga cgttttacag gccgtattcg acaaaggcac atcaaaattt cagctaactt
      421 atgatgacag agcgctacaa ttttttcgta cttttgtcct tgcaaccgaa caatcaacct
      481 atttcgagat ccctgccgaa gactcctggc tttttatcgc tgacggatct gataaagaac
      541 ttgattcctg tgccctttca ccaactataa acgaccactt tgcctttcat cctattgtcg
      601 atcccttatc gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagatagcc
      661 catcagccat agcggttggg cagcgtaaag acggggaaat ctacacagcg gatctcaaaa
      721 gtaaggcgct tgcattcacg atggcacacg cacttgagct cggtgataaa atgatttcaa
      781 tcaatctatt acctatgacc ctggttaacg aacctgacgc agtctctttt ttacttaatg
      841 aaataaaggc caatgctctg gtgcctgaac aaatcatcgt tgaatttact gaaagtgaag
      901 tcatatctcg gtttgatgag tttgccgaag cgattaaatc gctaaaggct gctggtatca
      961 gtgtagcaat tgatcatttt ggcgcaggtt ttgctggttt gttactcctg tcacgcttcc
     1021 agcctgacag aattaaaatc agtcaggaat tgattaccaa tgttcataaa agtgggccac
     1081 ggcaggcaat tattcaggcg atcataaaat gctgtacatc acttgaaatt caagtcagtg
     1141 ctatgggcgt ggcaacacca gaagagtgga tgtggcttga atccgcagga attgagatgt
     1201 ttcagggaga tctgtttgct aaagctaaat tgaatggtat cccttccata gcgtggccag
     1261 agaaaaaata attttcagca cattctttca catgatttca gtaaatcatc gtgaaaatga
     1321 atacgttacg tttaaattta atccacatta aaaataatgt ttcatccaat ttagctgaaa
     1381 tcttatttat actttgtaca attttagcgt atattgtaca aagtattatt gggtcgtgta
     1441 caggcgacgg agatttgtga ccgcaaggag gaattgtggc ttattacagc attggtgatg
     1501 ttgctgaacg ttgcgggatt aatcctgtca ctctccgggc ctggcaacgc cgctacggtt
     1561 tgttaaaacc acagcgcagt gaaggcggac accgactctt tgatgaagaa gacatacaac
     1621 gcatcgaaga gatcaagcgt tggataagta atggcgtccc tgtaggcaaa gttaaagcat
     1681 tactggaaac caccagccag gatacggaag atgactggag ccgcctgcaa gaagagatga
     1741 tgtcaattct tcgcatggct aatcctgcca aactacgcgc gagaattatt tcactgggtc
     1801 gagagtaccc agtagatcaa ttgattaatc atgtttacct tcctgttcgc cagcgtctcg
     1861 tgcttgatca caacacctcc cgcattatga gcagtatgtt tgacggtgca ttaattgaat
     1921 acgcagcaac ctcgcttttc gaaatgcgcc gtaagcccgg taaagaagcc attctgatgg
     1981 cgtggaatgt tgaagagaga gcacgattgt ggctggaagc atggcgttta tcattgtcag
     2041 gatggcacat ttctgttctt gctgatccca ttgaatcgcc gcgcccggaa ctgttcccga
     2101 cgcaaacatt gattgtctgg acaggcatgg caccaacgag aaggcaaaac gaacttttac
     2161 aacattgggg tgagcaaggt tataaagtca tctttcatgc cccctaattt cacctttatc
     2221 ccgcccctaa gacagaatgt tataatctga acccacaaca gcgtttgtac cggagctggt
     2281 ccgttgcaaa atgctcaccg ggagaattca gatattccct ctatcacact ctgtacatat
     2341 cacagccaaa ggacagacca gattgaagtg gtaaaaccga tcgtgcacac tacgcagtct
     2401 cacaactaac aagaggttat tatcctgatg gatcaggtcg ttgtttttca aaagatgttt
     2461 gagcaagtga gaaaagagca aaacttctcc tggttttatt cagaattaaa acatcaccgt
     2521 attgcacatt acatttatta tctggctacg gataacatca gaattattac tcacgatgac
     2581 acggttttgt tattaagagg aaccaggaac ctgttaaaag ttagtacgac caagaaccct
     2641 gctaaaataa aagaggccgc attgcttcat atttgcggaa aatctacatt tcgggaatac
     2701 tgttcaacac tagcaggcgc gggcgttttc cggtgggtta ctgatgttaa tcataacaaa
     2761 cgcagttact acgccattga taatacgctt ttatatattg aagatgtaga aaataataaa
     2821 ccattaatct agctaaagtt ggatgcttaa gaaatgcttc ataattcagt aaggcattag
     2881 cataatggga ataaaagtac agagactatc cctatggatg ataaatactg tccttttatt
     2941 gctacccat
//
LOCUS       DAZGVQ010000294         2918 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000294 DAZGVQ010000000
VERSION     DAZGVQ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.297"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(142..471)
                     /locus_tag="ACHLAG_005166"
     CDS             complement(142..471)
                     /locus_tag="ACHLAG_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HFP0658224.1"
                     /translation="MTNKLFEHTVLYDSGDAFFELKGNASMKLSPKAAIEVCNEAAKK
                     GLWILGIDGGHWLNPGFRIDSSASWTYDMPEEYKSKIPENNRLAIENIKDDIENGYTA
                     FIITLKM"
     gene            complement(461..721)
                     /locus_tag="ACHLAG_005167"
     CDS             complement(461..721)
                     /locus_tag="ACHLAG_005167"
                     /inference="COORDINATES: protein motif:HMM:NF023528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E5-related ribonuclease"
                     /protein_id="HFP0658225.1"
                     /translation="MPPGNFGVSGVQPLSNGATGTSFDKRSPKKTPPDYLGRNDPATV
                     YGSPGKYVVVNDRTGEVTQISDKTDPGWVDDSRIQWGNKNDQ"
     gene            complement(1098..1964)
                     /locus_tag="ACHLAG_005168"
     CDS             complement(1098..1964)
                     /locus_tag="ACHLAG_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP0658226.1"
                     /translation="MIKGVLFAFLAAFSWGAAIVMSKKGLENMDAGGLFFWQVGSAAM
                     LSWFVLAISRKKLPVTKKSILAYSTGIFEPFLAYTFTLYGLKFISAGITAVIFSLESV
                     FILILSVILFFVKINSPLHFILLLTGAMAGSIMVALPDINDRIDNITGYILIVAGVLS
                     ASFYVVISSRLIVNFEPVTLLTGQLTFSFILACFFIVLTGTSLQLPVESVFLVFLSGI
                     LQYFLAFCFYLYSLRWIPVHIAGAMLYFIPLVALLLSWFFLQEDISFIQGLGVFITIL
                     CVYILNKKYAIE"
     gene            complement(1957..>2918)
                     /locus_tag="ACHLAG_005169"
     CDS             complement(1957..>2918)
                     /locus_tag="ACHLAG_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658227.1"
                     /translation="DDNIIEKELRKWASVNQPCVFGKLACKKIKGLDFHLSIIKDPSL
                     YTNDSKLFEFLRRERIIFKERALKGEVSAHLIYFIHQKIAFAKPSKELVDIQKHICSL
                     HMPEYYPVREDIIYTESIPLQENENVMVYKAGVNIFYSSAHRTRNHDRRIPGGMLISV
                     NAPGHFMRLAIAKGFYKDQEQALSDIRNMTIQSIGKGGYSHPEKISTTWHSDNKMNRF
                     GCPIHSTNSDYYSGFYHTDVLIPGELTQDSRIIHNVDSNDPMIFNWNVLFYVSLEKFP
                     VDNPYYGEFLGIPVDEEAKFFNPFPPRAFENKPLYNEEITKYD"
BASE COUNT          975 a          576 c          491 g          876 t
ORIGIN      
        1 atggctgcaa gaagcaataa aataatccat gttatttttt tcatactgtt ccttgtatat
       61 atggactcag gcattcatcc ataaaaacct gaaatccaaa ggttgaaatc atccatgaaa
      121 cataaaattc tataacacta tttacatctt taacgtgata atgaaagcag tgtatccatt
      181 ctcaatatca tctttaatat tttcaatagc caatctatta ttttcaggga tttttgattt
      241 gtattcctcc ggcatatcat atgtccatga tgctgaacta tctatcctga atccaggatt
      301 cagccaatgc ccaccatcaa tgcccaaaat ccataagcct tttttcgctg cttcattaca
      361 aacttctatt gcagcttttg gtgataactt catagaagca tttcctttta attcaaaaaa
      421 ggcatcacca ctatcatata acaccgtatg ttcaaataat ttattggtca tttttatttc
      481 cccattgaat tctcgaatcg tccacccaac ccggatctgt cttatcacta atctgagtaa
      541 cctcaccagt acgatcattg acaacaacat atttaccagg agaaccatac actgttgctg
      601 gatcatttcg acctaaatag tctggaggtg ttttcttcgg actacgctta tcaaaagatg
      661 taccagttgc accgttagat aggggttgta cgccggaaac gccgaaattt ccgggaggca
      721 caaattttat gcttggcaac ctgtacgtaa gaccgccgtt aaccggacag caaagaaata
      781 gctgtttaga gcgaaagaat cactgcccgt aacgtatcac atagctggta cggataaatt
      841 ctcacttact gcaccataaa cgtactcgtc aacaaccgcc aaacatgctt caaaaatcaa
      901 caaattcgct gtatcggaaa tttcggcgtt tccggcgtat aaccccctat aaatatattg
      961 cagcagccaa agtcggataa aaaccacatt caagaaatta cggtttcatc ctttgttcag
     1021 cagttgaaac cgtaagtttc tgttcaaatg acccgctggt ccatattgta cgtttcccaa
     1081 acgcagccct aataaagtca ttcgatggcg tatttcttat tgagaatata tacgcataaa
     1141 atagttataa aaacgcctag tccctgaata aatgatatat cttcctgcag gaagaaccag
     1201 gacaacagca atgctaccag aggaataaaa tagagcattg ctcctgctat gtgaacggga
     1261 atccagcgta gggaataaag atagaaacaa aaagccaaaa aatattgtaa aattcctgac
     1321 aaaaacacaa gaaatacaga ttctacaggc agttgcaggg atgtgccagt tagaacaata
     1381 aaaaaacaag caagaataaa agagaaagtc aactgcccgg ttaacaacgt aacaggctca
     1441 aaattcacaa taagtcgtga tgagataaca acataaaagg atgctgagag tacacctgca
     1501 acaataagta tatatcctgt aatattatca atgcggtcat tgatatcagg tagggcaacc
     1561 attatactgc ctgccattgc tccagtaagc aataatataa agtgtaatgg tgagttaatt
     1621 ttaacgaaaa ataatataac agatagtata agaataaata ctgactcaag tgaaaaaata
     1681 acggctgtta taccagcaga aataaatttc aggccataga gtgtgaatgt atacgccaga
     1741 aatggctcaa aaattcctgt tgaataggca aggattgatt tttttgttac cgggagcttt
     1801 tttctgctaa ttgcgagaac aaaccatgat aacattgcag cactaccaac ttgccagaaa
     1861 aacaatcctc cggcatccat gttttcgaga ccttttttgc tcatcactat tgctgctccc
     1921 catgaaaaag cagcaagaaa tgcaaacaga actcccttaa tcatatttag ttatctcctc
     1981 attataaaga ggtttatttt caaacgctct tggtggaaat ggattaaaaa actttgcttc
     2041 ttcatctaca ggaataccaa gaaattctcc ataataagga ttatcaacag gaaacttttc
     2101 cagtgagaca taaaacaata cattccagtt aaaaatcata gggtcattgc tatcaacatt
     2161 atggattatt ctggagtcct gagtcagttc tccgggaatt aatacatctg tatgataaaa
     2221 gcctgaatag taatcactat tagtagaatg tatagggcag ccaaagcgat tcattttatt
     2281 atctgagtgc catgtggttg atattttttc tggatgagaa tatcctcctt tacctatgga
     2341 ttgaatagtc atattacgga tatcagataa agcttgctcc tgatctttat agaatccttt
     2401 tgcaattgcc agtctcataa aatgtccagg tgcatttaca gatataagca ttccaccggg
     2461 gattcgtcgg tcatgatttc gggttctgtg agcagaagag taaaaaatat taactccagc
     2521 tttatatacc atcacgtttt cattttcctg caatggtatg ctttctgtgt agattatatc
     2581 ttctcttaca ggataatact caggcatatg taaggaacat atatgtttct gtatatcaac
     2641 aagttcttta gagggttttg caaaagcaat tttctgatgt ataaagtaga taagatgagc
     2701 acttacttcc cctttaagag ctcgttcttt gaagataatc cgttctctac gtaaaaactc
     2761 gaagagttta ctatcattag tatacaatga tggatctttt attattgata agtgaaaatc
     2821 aaggcctttg attttcttac atgcaagttt accaaaaaca cacggttgat ttactgatgc
     2881 ccattttctt agctcttttt caattatatt atcatctg
//
LOCUS       DAZGVQ010000295         2915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000295 DAZGVQ010000000
VERSION     DAZGVQ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.298"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            291..968
                     /gene="tnpA"
                     /locus_tag="ACHLAG_005170"
     CDS             291..968
                     /gene="tnpA"
                     /locus_tag="ACHLAG_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFP0658228.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
     gene            968..1315
                     /gene="tnpB"
                     /locus_tag="ACHLAG_005171"
     CDS             968..1315
                     /gene="tnpB"
                     /locus_tag="ACHLAG_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP0658229.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            1335..2906
                     /locus_tag="ACHLAG_005172"
     CDS             1335..2906
                     /locus_tag="ACHLAG_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISCro1 family transposase"
                     /protein_id="HFP0658230.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPALPS
                     ALRQSSARKPLPASLPRETQVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTYITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKVDLSSQ"
BASE COUNT          706 a          788 c          790 g          631 t
ORIGIN      
        1 ttaagttgcc agagaaattt ttccggacgg atgctgataa taatgatgta atttttcaag
       61 atttctggaa atctgtattc tgcacaggcg caattgtatg ccgcctttaa aacttcaatt
      121 cagtgactca cctgccattc aaattttcgg ataccagaca accatgcctt atatcgatat
      181 aacaactatg cgcgggatga tgccaggcgt tattgcatct atgctgccag atcattcgta
      241 accggctcat ttaaaccgtc tggtctgttt cctccggctc tacaaaaata atgtccatca
      301 tttttaatgg acactatcgt atgaaacacc ggacctggat cactgaagct ttacgtcttc
      361 actttgaaga acatttaccc cgggttgtgg ccgggcgtcg cctgggtgta ccaaaatcaa
      421 cagtttgtag tatgttcgtg cgctttcgga gagctggcct ttcgtggcct ttgcccgcag
      481 gcatgtcgga gcaggaactt gatgcctgcc tttacggaca attttccacg gtaccagtcg
      541 tacgtcctga aagcaccgtt atatccgaag cccccgtggt aaaaaaacgt ccccggcggc
      601 ccaacttccc ttatgagttt aaaatcgcct tagtggagca gtcactgcag cccggagcct
      661 gtgtggcgca gatcgcccgg gaaaacggaa tcaacgataa cctgctcttc aactggcgcc
      721 atcaataccg gaaaggtggc ctgctgcctt ccggaaaaaa tatgccggca ctgcttcccg
      781 tgacgttaac gccggagccg gataataaaa tcccggcccc cgcacaggaa ccagagcaga
      841 taaatacacc gtccgacagt ctgtgttgtg agctggttct gccggccgga actctcaggc
      901 ttaaaggtaa actgacgccg gcgttattac agacacttat ccgcgaaata aaagggagca
      961 gccactgatg atatctctcc ctgcaggttc gcgtatctgg ctggttgcag gtatcaccga
     1021 tatgcgaaat ggctttaacg gcctggcatc aaaagttcag aacgtcctga aggatgaccc
     1081 gttctccgga cacctgttca tcttccgcgg acgtcggggt gaccagataa aagtgttgtg
     1141 ggctgacagt gacggactgt gcctcttcac caaacgcctg gagcggggcc gcttcgtctg
     1201 gccagtcacc cgtgacggca aggtgcacct tactccggct cagttatcca tgcttcttga
     1261 aggtatcaac tggaagcacc cgaaacgaac ggaacgcgct ggaatccgca tataacccgt
     1321 tgtaaagtga ggatatggac acctcacttg ctcatgagaa cgcccgcctg cgggcactgt
     1381 tgcagacgca acaggacacc atccgccaga tggctgaata caaccgcctg ctctcacagc
     1441 gggtggcggc ttatgcttcc gaaatcaacc ggctgaaggc gctggttgcg aaactgcaac
     1501 gtatgcagtt cggtaaaagc tcagaaaaac ttcgtgcaaa aaccgaacgg cagatacagg
     1561 aagcacagga gcgaatcagc gcacttcagg aagaaatggc ggaaacgctg ggtgagcagt
     1621 atgacccggc actgccatcc gccctgcgcc agtcttcagc ccgtaaaccg ttaccggcct
     1681 cacttccccg tgaaacccag gttatccggc cggaagagga atgctgtcct gcctgtggtg
     1741 gtgaactcag ttctctggga tgtgatgtgt cagagcaact ggagcttatc agcagcgcct
     1801 ttaaggttat cgaaacacaa cgtccgaaac tggcctgttg ccgttgtgac catatcgtgc
     1861 aggcgccggt gccttcaaaa cccattgcac gcagctatgc cggagcgggg cttcttgccc
     1921 acgttgtcac cggaaaatac gcggaccatc tgccgttata ccgccagtca gaaatatacc
     1981 gtcgccaggg cgtggagctg agtcgtgcca cgctggggcg ctggacaggt gctgttgctg
     2041 aactgctgga gccgctgtat gacgtcctgc gccagtatgt gctgatgccc ggtaaagtcc
     2101 atgctgatga tatccccgtc ccggtccagg agccgggcag cggtaaaacc cggacagccc
     2161 ggctgtgggt ctacgtccgt gatgaccgta acgccggttc acagatgccc ccggcggtct
     2221 ggttcgcgta cagtccggac cggaaaggta tccatccaca aaatcacctg gccggttaca
     2281 gcggtgtgct tcaggccgat gcttacggtg gttaccgggc gttatacgaa tccggcagaa
     2341 taacggaagc cgcgtgtatg gctcatgccc ggagaaaaat ccacgatgtg catgcaagag
     2401 cgcccaccta catcaccacg gaagccctgc agcgtatcgg tgaactgtat gccatcgagg
     2461 cagaggtccg gggctgttca gcagaacagc gtctggcggc aagaaaagcc agagccgcgc
     2521 cactgatgca gtcactgtat gactggatac agcaacagat gaaaacactg tcgcgtcact
     2581 cagatacggc aaaagcgttc gcatacctgc tgaaacagtg ggatgcactg aacgtgtact
     2641 gcagtaatgg ctgggtggaa atcgacaaca acatcgcaga gaacgcctta cggggagtgg
     2701 ccgtaggccg gaaaaactgg atgttcgcgg gttccgacag cggtggtgaa catgcggcgg
     2761 tgttgtactc gctgatcggc acatgccgtc tgaacaatgt ggagccagaa aagtggctgc
     2821 gttacgtcat tgaacatatc caggactggc cggcaaaccg ggtacgcgat ctgttgccct
     2881 ggaaagttga tctgagctct cagtaaatat caata
//
LOCUS       DAZGVQ010000296         2886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000296 DAZGVQ010000000
VERSION     DAZGVQ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.299"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            44..676
                     /gene="comR"
                     /locus_tag="ACHLAG_005173"
     CDS             44..676
                     /gene="comR"
                     /locus_tag="ACHLAG_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFP0658231.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(738..1277)
                     /gene="ycfJ"
                     /locus_tag="ACHLAG_005174"
     CDS             complement(738..1277)
                     /gene="ycfJ"
                     /locus_tag="ACHLAG_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP0658232.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1487..2791)
                     /gene="ndh"
                     /locus_tag="ACHLAG_005175"
     CDS             complement(1487..2791)
                     /gene="ndh"
                     /locus_tag="ACHLAG_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFP0658233.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          703 a          784 c          702 g          697 t
ORIGIN      
        1 ttataataac ctttcattca cagtcaccag gtacaacggc atcatggcaa ctgactcaac
       61 acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg ccgcgcttga
      121 taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg cggacctcgt
      181 cgaagcgacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca aagaggggtt
      241 atttcgtgcc gttctcgacc gctatatcga tcgttttgcc gctaagcatg aagcacagct
      301 gttttgtgaa gagaaaagcg tggagtctgc gctggctgac tattttgctg ccatcgccaa
      361 ctgctttacc agcaaagaca ccccggcagg ctgcttcatg atcaacaact gcaccaccct
      421 ctccccagat tcaggagata tcgccaatac gttgaaatca cgccatgcga tgcaagagcg
      481 cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga cccactgtga
      541 cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga tcagcgcacg
      601 cgaaggtgca tcgctggaaa aactaatgca gattgccgga acgactttgc gtttatggcc
      661 cgaactggtg aaataagtcg caaaaaaagc ccctcagcct gagcgaatga ggggccaaat
      721 tgcagagtac agccttgtta tactttgtta ttcaaaatca gttgcccatt actatctagc
      781 gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc gccaatctta
      841 taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt acaacgctgt
      901 tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt accggcatat
      961 ccaccaccca gcgcccccac aacagtggcg acatctttac cgcgaccacc gccaaactga
     1021 tgccctatca cgccgccagc aacagcgccg agcaccgacc cggtaatgcg attttcatcc
     1081 tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg tgttttaacc
     1141 gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccacg ttcaaacacg
     1201 ttcagactgg ccactgccgc tacgcccagc gcagctgcga caccaatccc gatacccgcc
     1261 aacattgatt tattcacggg aacacctcct tctttgcagt tatgccaatc atgcaagcga
     1321 aggagaaaag cagctatcag acagaagatg aaaagcgcgg gaatggctgg aaatacttaa
     1381 ggattcggag gagtctgaag gaatgcctga tgcgacgcgg ctgccgatcg cgctgttcct
     1441 caagggcgga taaggcgttt atgccacatc cgccagtgta cgtcgattaa tgcaacttca
     1501 aacgcggacg gataacgcgg ttaatactcc ccaccagcat cattaatccg gttttaaagt
     1561 aaccatgcag cgcaatctga tgcattcggt atagcgagat atatacaaag cgcgcaattc
     1621 gtccttcaat catcattgag ccgcgcgtca ggttacccat caggctacca acggtggaga
     1681 agttcgacag cgataccagc gaaccatgat ctttatactg ataatttttc agcggcttac
     1741 cattcatctg cgccagaatg ttgttcattg cgcaagtcgc catctggtgt gcagcctgag
     1801 cacgcggcgg aacaaagccc ccttccggac gcgggcatga cgcgcagtcg ccaatagcgt
     1861 aaatgtctgg atcgcgggtg gtttgcagcg tcggttccac caccagctgg ttgatacggt
     1921 tagtttcaag accaccgata tctttcagga agtctggcgc tttgatcccg gctgcccaca
     1981 ccatcagatc agcctcaata tattcgccat ctttagtgtg caggccgcct tcatcagcac
     2041 tggtgaccat ggtttgcgtc agcacgcgaa cgccaagttt cgttagctcg ttgtgggccg
     2101 cagcagagat acgtggcggt aatgcaggca aaatacgttc tcccgcttct accagcgtta
     2161 cgttcagggc ttcgttggtc aggcctttgt aaccgtagct gtgcagttgc ttgaccgcgt
     2221 tgtgcaattc agcggagagt tctacacccg tcgcgccgcc gccgacaatc gcaatgttca
     2281 ctttgccatt tgcgcccagg ttggcggagt atttcaggaa gagattcagc atctcctggt
     2341 gaaagcgacg cgcctggtgc gggttatcga ggaaaatgca gttctctttg acgcctggcg
     2401 tattgaaatc gttagaggtg ctacccagcg ccatgaccag ggtgtcatag gcgattttac
     2461 gttccggaac cagcagttca cctttctcgt cgcgtagttc tgcaatagtg attgttttcg
     2521 cttcacgatc aatatcaatg acggaaccca actggaactg gaaaccatga ttgcgcgcat
     2581 gggccagata gctcaacgca tcgacgcctt catcaagcga gccagtcgcc acttcgtgca
     2641 gcagcggttt ccacaggtgg ctgtggttac gatcgaccag cgtaattttg gcttttttct
     2701 tacgtcccag cttatgcccc agctgtgttg ccatttccag cccaccagca ccgccgccga
     2761 caatcacaat ctttttcaat ggcgtagtca acgtgacccc cttaaattta ttaaccaatt
     2821 gttaattaat ggttattaac atagctctta attaacaaca ggttacagca atgaaaatgt
     2881 tcttcg
//
LOCUS       DAZGVQ010000297         2868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000297 DAZGVQ010000000
VERSION     DAZGVQ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.300"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..79)
                     /locus_tag="ACHLAG_005176"
     CDS             complement(<1..79)
                     /locus_tag="ACHLAG_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310734.3"
                     /note="induced by CusR in the presence of copper; YedW
                     induces the expression of the upstream gene yedV (encoding
                     a sensor kinase) as well as yedW; yedVW is one of four
                     copper regulons found in E. coli; part of the copper
                     homeostasis mechanism; confers resistance to copper and
                     several drugs when induced; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFP0658234.1"
                     /translation="MSFEDSEKAARVTLQQHYNFVMNQAV"
     gene            101..514
                     /gene="hiuH"
                     /locus_tag="ACHLAG_005177"
     CDS             101..514
                     /gene="hiuH"
                     /locus_tag="ACHLAG_005177"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFP0658235.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            623..1627
                     /gene="msrP"
                     /locus_tag="ACHLAG_005178"
     CDS             623..1627
                     /gene="msrP"
                     /locus_tag="ACHLAG_005178"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFP0658236.1"
                     /translation="MKKNQFLKESDVTAESIFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPGAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            1628..2263
                     /gene="msrQ"
                     /locus_tag="ACHLAG_005179"
     CDS             1628..2263
                     /gene="msrQ"
                     /locus_tag="ACHLAG_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFP0658237.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            2520..>2868
                     /locus_tag="ACHLAG_005180"
     CDS             2520..>2868
                     /locus_tag="ACHLAG_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZinT/AdcA family metal-binding protein"
                     /protein_id="HFP0658238.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVE"
BASE COUNT          760 a          678 c          663 g          767 t
ORIGIN      
        1 aaacagcctg gttcattaca aaattgtaat gctgctgtaa ggttaccctg gccgcttttt
       61 cgctatcctc aaaactcatt cacatgacaa ggatataaac atgttaaagc gttatttagt
      121 actctccgta gtaacggcag cattttcatt accttctttg gtttatgccg cacaacaaaa
      181 cattcttagc gtgcacattt tgaaccagca aaccggaaaa cccgctgccg acgtgacagt
      241 cactcttgaa aagaaagcgg acaacggctg gttacaactt aataccgcca aaacagataa
      301 ggatggacga attaaggcac tgtggcccga gcaaactgca actacgggcg attaccgtgt
      361 cgtatttaaa accggggact atttcaagaa acaaaatctt gaaagtttct tccctgagat
      421 ccccgttgaa tttcatatta ataaagtgaa cgagcattat catgtgcctt tattacttag
      481 ccaatatggg tattcaacct atcgtggcag ttaatgcata aatatgtaaa agcagatctc
      541 tgctactgtt agggaggaca ctccctgaca gattaacagt aaacggctct tgctggctaa
      601 cgacaaaaaa gtgtgatggc ttatgaaaaa gaatcaattt ttaaaagaat cagatgttac
      661 ggccgagtcg atattcttta tgaagcgtcg acaggtgtta aaagcactgg gcatcagcgc
      721 agctgcactt tctttgcctc acgctgcgca tgccgatctg cttagctggt ttaaagggaa
      781 cgatcgcccg cccgcccccg ccggaaaacc gctggagttc agcaagcctg gcgcctggca
      841 aaataacctg ccactgacgc cagtagataa agtctccggt tataacaact tctatgaatt
      901 cgggctggat aaagccgatc ccgccgctaa tgctggtagc ctgaaaaccg atccatggac
      961 actgaaaatc agcggcgaag tggcaaaacc attgaccctc gatcacgatg atttaacccg
     1021 tcgcttcccg ctggaagagc gtatttatcg tatgcgctgc gtggaggcat ggtcgatggt
     1081 ggtgccgtgg attggttttc cgctgcacaa attgctggcg cttgccgaac ccaccagcaa
     1141 tgcgaagtat gtcgctttcg aaacaattta tgcaccggaa cagatgcctg gccagcagga
     1201 ccgctttatc ggcggcgggc tgaaatatcc ttatgtcgaa ggattgcgtc tcgacgaagc
     1261 aatgcatccg ctcacactga tgaccgtggg tgtttatggc aaggcgttac cgccacaaaa
     1321 tggcgcgccg gtacgactga ttgtgccgtg gaaatatggc tttaaaggga ttaaatcgat
     1381 agtcagtatt aagctgaccc gcgagcgtcc gccaaccacc tggaatctgg cagcgcctga
     1441 cgaatacggt ttttacgcca acgttaatcc gcatgttgat cacccgcgct ggtcacaggc
     1501 taccgaacga tttattggtt caggcggcat cctcgatgtt cagcgccagc caacgctact
     1561 gtttaatggt tacgccgacc aggtggcatc gctgtatcgt ggcctggatt tgcgggagaa
     1621 tttctgaatg cgtctgacag caaaacaggt gacatggctg aaagttagcc tgcatcttgc
     1681 cggattgttg ccgtttctct ggctggtctg ggcgatcaat cacggtggac tgggtgccga
     1741 tccggtgaaa gatattcagc attttaccgg tcgcactgca ctgaaatttt tgctggcgac
     1801 cttgttaatc acacctctgg cacgctacgc aaaacagccg ttattgatac gcactcgccg
     1861 cctgttaggc ttatggtgct ttgcctgggc gacactgcat ttaaccagtt acgcattgct
     1921 ggagctgggc gtgaataatc tggcgttatt aggcaaggag ttaattaccc ggccttattt
     1981 aacgttagga attatcagct gggttatttt gcttgcttta gcgtttactt caacccaggc
     2041 gatgcagcga aaactcggca agcactggca acagttgcat aatttcgtct atcttgtcgc
     2101 gatcctggcc ccaatacatt atctgtggtc agtgaagatt atttcaccgc agccgctcat
     2161 ctacgctggg ctggctgtac tgcttttagc cttacggtat aagaagttgc gttccctttt
     2221 taaccggtta cgcaagcagg ttcacaacaa attatctgta taacttcata ttaccgacaa
     2281 agtacgcgtt atccctgccg gatgcggcgc gagcgcccta tccggcctac gcagatcaca
     2341 tcaggttatg tttcgtttgt catcaacctc aaattatcaa cataattcat tttatttcaa
     2401 taagatagat aagtatacct gagaaaacta tgctctcatc tcctaagagt tattgcattt
     2461 tgtcatatgt tacaatataa cattacacat catatacaat gactctggag gaaactgttt
     2521 tggcgattcg tcttcacaaa ctggctgttg ctttaggtgt ctttattgtt agcgctcctg
     2581 ccttttcgca tggtcatcac tcacacggca aacccttaac agaggtcgaa caaaaagctg
     2641 ctaatggtgt ttttgatgat gccaatgtac aaaaccgaac gctcagtgac tgggatggag
     2701 tctggcaatc cgtttatcct ttactgcaaa gtggcaaact tgaccccgtc tttcagaaga
     2761 aagcggatgc agataaaact aaaacatttg ctgaaattaa agattattat cacaaaggtt
     2821 atgcaacaga tatcgagatg attggcattg aggacggcat tgttgaat
//
LOCUS       DAZGVQ010000298         2852 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000298 DAZGVQ010000000
VERSION     DAZGVQ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.301"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(110..1126)
                     /locus_tag="ACHLAG_005181"
     CDS             complement(110..1126)
                     /locus_tag="ACHLAG_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010849026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFP0658239.1"
                     /translation="MKNPDSPVLSLRDYSTRDTKWDSDRVMADRVAQIYENDSMFSSR
                     GERMFDCSRRLLFAPKVSRLTGEMKLALRKGEFCHVPFCPVCSRRRSLRWMRRLWEAL
                     PKLLVERPAARWLFMTLTVKNPPVENTRETLIRMNAAWKRLSDRKEFRSVLGWLRTTE
                     ITYGKVPGCCHPHFHVLMMVPPSMLSGNGYVKHARWVEIWSECLRVDYEAGVDIRVVK
                     PKQGWKRPDGVTLPDMHRAALESGVIETMKYTVKSSEVVRDPAWFLELARQTYGLRMV
                     ATGGRLKEVLKVDKPETDEDLVGADIPAEPDEFEEQAFWLAFDWWRDEKRYKRNPKAD
                     KKKD"
BASE COUNT          709 a          846 c          650 g          647 t
ORIGIN      
        1 aaaaaaaacg actaaatcct tgcccgtttc tgaaaatcac attagaacgc ttctgagcgc
       61 gtttctgaat attctcccag tgcatgaata gttcgcgcct gaaacgcgct caatccttct
      121 tcttgtcagc tttcggattc cgcttatagc gcttctcatc ccgccaccag tcaaacgcca
      181 gccagaaggc ttgctcctcg aactcgtccg gttctgccgg aatatctgcc ccaaccaaat
      241 cctcgtctgt ctccggttta tcgactttca gaacctcttt gagacgaccg ccggttgcca
      301 ccatccgaag cccgtaggtc tgccgcgcca gctccagaaa ccacgccgga tcacgcacaa
      361 cttccgaaga tttcaccgtg tatttcatcg tctcaatgac gcctgactcc agcgccgcac
      421 gatgcatatc aggcagggtt acaccatccg gccgtttcca gccctgtttc ggttttacca
      481 cccgaatatc cacacccgcc tcataatcga cacgaaggca ttcactccag atctccaccc
      541 agcgagcatg tttcacataa ccattaccgg acaacatcga aggggggacc atcatcagaa
      601 cgtgaaaatg cggatggcaa catcctggca ccttcccgta agtaatttcc gtcgtacgta
      661 accagccaag aaccgacctg aattccttac gatcactcaa gcgtttccat gctgcattca
      721 tccgaatgag tgtttcgcgg gtattttcca caggaggatt tttgacagtc agcgtcatga
      781 acaaccagcg ggccgcagga cgttcaacca gcaattttgg caaggcctcc cagaggcgac
      841 gcatccagcg gagagagcga cgacgactac acaccggaca aaacggaaca tgacaaaact
      901 cccccttccg aagggccagc ttcatttcgc cagtcaggcg ggacaccttc ggcgcaaaca
      961 acaaccgacg ggaacaatca aacatccgtt ctccacgaga ggaaaacatc gaatcatttt
     1021 cgtaaatctg cgccaccctg tcagccatca ctcgatcgct gtcccatttt gtatcccgcg
     1081 tgctgtaatc acgtaacgag agtaccggac tgtctggatt tttcatttgc accccccaag
     1141 aactgggcag atgcggaaat tttgtgttag catttttctg tcacctgtga ctctgttgcc
     1201 gaacaacgaa ttccaaactg gtgatcgacc cctggagtgt taccgcactc gcagggaccc
     1261 ctccccgagc tgtttatttt ttaaccatct ttttttatct cttttccatc tatttttacc
     1321 gctcaaagcc gcgccaccac tgggctaagc caaaataaca cagaccgcag ttgttgccta
     1381 tagtatatat acaaggaaac gcgcgcgaag acctcgccaa ggtagactat tttttgttcg
     1441 attttccgag aacaaaaaca acaaccacga aagcgttttc ccgacaaaac acccacaggc
     1501 ttttccaccg attttgtgaa caacaaaaaa cctgcctcgc aggtggttgg tcccactgcg
     1561 tgacaccaac caccatcacc agtgtttttt ctcgtgccgt cgtcaaacac aaaatcggaa
     1621 cccacgccgg aggcgcggga ccccacgatt ttctgtttga cgagtcataa aacgtctcac
     1681 gctgacagat ttcaatctga ctgcgtgaga cgttggcggg gtgtcggggc gcagccctga
     1741 ccaggtggca tttgtctgat tgcgcgtgcg cggtccgaca aatacacatc ctgtcccgac
     1801 tttttctccg aaatgctcgc agaggggcgg cagttttgtg ccgttcagcc ctccgcagga
     1861 gcgcgctgtt tctcgacgaa ggagagaaac gtctaagtgc gcatccgcgc ttaacgcgcg
     1921 gatgattttt ggcaccgcag ccaccacggc cgcgcaaaat tctggcgaac ggacagcaaa
     1981 tcattttgcc gtttttgtgc cgcagccgtt ccgcgcgatc gacaaactgg cagtcggggg
     2041 aatctcacga agtgcgcact tatacaatct caacttcgtt tcgattgtgt gcggtctgcg
     2101 acgcggtgaa aaccgcagat aacaggcaaa aaaatccccc tgtgtcaggg ggccgtcaaa
     2161 gtcaatttcc tgatgatgat agttattttg aggtgttgct atgcccgctc aatttgccgc
     2221 aggaacaccg caaaaacaac tccgatgacg ggacaggatg tgggaatgtc ggaccgcgca
     2281 cgcgcactca gacattcccc acctggtcag ggctgcgccc cgacaccctc gacaacctgc
     2341 ccgggacacg ttaaaacgac atctgcgcgt ttctggagcg cccgttaacc atggggatgg
     2401 tattagcgcc gggatgcgac agaaacgcgc ttaaaacgcc cctgaacgca tcaaacgcga
     2461 aaaaacgaat aaaaccttgc ctgtctgcaa aaatcgcgtt agaacgcgtc tgagcgcgtc
     2521 tgaggggtta aaattcactg tgcgtcaaat ggggattgtt acaccacgcc ctcaaacgcg
     2581 ctcacttccc cttcccgtct acatgcggaa atgttgttgt tttgtatctt ttatgttaag
     2641 catcggatga gcgtgtttct tatcgcattt ccactatcat caacccgcga agacctgtag
     2701 aaacacgctc accccccccg actactggac agcagaatcg caccgcgtta aacatgacat
     2761 gcgcgtttct ggtgagcgaa aagctgtcag ccgtggattg ccgtagcaga gacccagaaa
     2821 cgcgcttaaa acgcttccga gtgcgccaaa ac
//
LOCUS       DAZGVQ010000299         2806 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000299 DAZGVQ010000000
VERSION     DAZGVQ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.302"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            88..2355
                     /gene="ydeP"
                     /locus_tag="ACHLAG_005182"
     CDS             88..2355
                     /gene="ydeP"
                     /locus_tag="ACHLAG_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFP0658240.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLSWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGITQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSNTTIYGMDDRYRGVFGQRDVVFMSAKQAKICRV
                     KNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHDPLSG
                     IPGYKSIPVELEPSN"
     gene            2603..2800
                     /gene="safA"
                     /locus_tag="ACHLAG_005183"
     CDS             2603..2800
                     /gene="safA"
                     /locus_tag="ACHLAG_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFP0658241.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLMLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
BASE COUNT          747 a          654 c          682 g          723 t
ORIGIN      
        1 atccatgttg gcgataatcc ctttgtatgt actgtgcatc atcgctatta caaatcctaa
       61 taattcattt ccacacagga taagtagatg aagaaaaaaa ttgaatccta ccagggtgct
      121 gcaggtggtt ggggtgctgt caaatccgta gcgaatgcag tacgtaagca gatggatata
      181 cgccaggatg ttattgccat gtttgacatg aataagccag agggctttga ctgtccgggt
      241 tgtgcatggc cagatcctaa gcacagtgcg tcattcgaca tttgtgaaaa cggcgcaaaa
      301 gcaatcgcct gggaagtcac ggataagcag gtaaatgcct ctttctttgc tgagaatacg
      361 gttcaatcat tactttcctg gggagatcac gagcttgagg ctgcgggacg tctcactcag
      421 cctttgaaat atgatgccgt cagcgactgt tacaagccat taagctggca acaagctttc
      481 gacgaaattg gcgcacgcct tcaaagctat agtgatccca atcaggttga attctatact
      541 tcgggccgca cttccaatga agctgccttt ctttatcagc tttttgcccg tgaatacggg
      601 agcaataact ttcccgactg ctccaacatg tgccatgaac cgacaagcgt gggtttggca
      661 gcgagtatcg gtgtaggtaa agggaccgtg ttgctggaag actttgagaa gtgcgattta
      721 gtcatttgca ttgggcataa ccctggtaca aaccaccctc gcatgctgac ttcgttgcgc
      781 gctttagtga aacggggagc gaaaatgatc gccatcaatc ctctacagga acgtggcctg
      841 gagcgattta ccgcaccgca aaacccgttt gaaatgctga cgaactctga gactcagttg
      901 gccagtgcct actacaacgt gcgcattggt ggcgatatgg cgttgctcaa ggggatgatg
      961 cgcctgttaa ttgagcgcga tgatgctgca agtgccgcag gtcggccctc attgcttgat
     1021 gacgaattta ttcaaacgca taccgtcggc tttgacgagc tacgccgtga cgttctcaat
     1081 tccgagtgga aagatatcga acgtatttct ggactaagtc agacacaaat cgccgaactg
     1141 gctgacgcat atgccgctgc cgaacgcacc attatctgtt acggaatggg gatcactcag
     1201 cacgaacatg gtacccagaa cgtacagcaa ctggtcaatt tgctgttgat gaaaggtaac
     1261 attggcaagc ctggtgcggg tatctgccca ctacgtggtc actctaatgt acagggcgac
     1321 cgaaccgtcg gtatcaccga gaaaccgtct gcagagtttc tggctcgcct gggtgagcgc
     1381 tatggcttca ccccacctca tgcacctgga catgctgcaa ttgccagcat gcaagcaata
     1441 tgtacggggc aggctcgagc attgatctgc atggggggca actttgcact ggcaatgcca
     1501 gatcgggaag cgagcgctgt accgttaacg caattagatt tggcggtaca cgtagccact
     1561 aagcttaacc gctctcatct gctgaccgca cggcatagct atatcctgcc ggttctgggg
     1621 cgtagcgaga ttgatatgca aaaaagcggt gcgcaggcgg taaccgttga ggattcaatg
     1681 tcgatgattc atgcctcgcg tggcgtgtta aaacccgccg gtgtaatgct gaaatcagag
     1741 tgtgctgtgg tcgcgggaat cacccaggca acactacccc agagcgtggt agcctgggag
     1801 tatctggtgg aagattatga tcgcattcgc aatgacattg aagctgtgct gccagagttc
     1861 gccgactata accagcgcat ccgtcatccc ggtggttttc acctgataaa tgcagctgct
     1921 gaaaggcgct ggatgacgcc gtcaggtaag gctaatttca ttaccagcaa agggctgtta
     1981 gaagatccct cttcagcgtt taacagtaag ctggtcatgg cgacagtacg cagcaacacg
     2041 acgatttatg gtatggatga tcgctatcga ggggtattcg gtcaacgaga tgtggtcttt
     2101 atgagtgcta aacaagctaa aatttgccgt gtaaaaaacg gcgaaagagt taatcttatt
     2161 gcgcttacgc cagacggtaa gcgcagctca cgccgcatgg atagattaaa agtggtcatt
     2221 taccctatgg ctgaccgctc actggtgacc tattttccag aatcgaatca catgctaact
     2281 cttgataacc acgatccatt aagtggcatt cctggttata aaagtattcc ggttgaatta
     2341 gaaccatcaa attaattcgc ctcctcattt cttctgctgt catccgcaca gcagaagaat
     2401 ttctcattga ctattatcgc gcaatttgct cacattgatt aaattaaacc acacactata
     2461 agatataaac ctctgcctac agctgtaaga aactccgctc agtactgaag tgccagtcct
     2521 atttcctctt ttctccagcc tgttatatta agcatactga ttaacgattt ttaacgttat
     2581 ccgctaaata aacatatttg aaatgcatgc gaccacagtg aaaaacaaaa tcacgcaaag
     2641 agacaactat aaagaaatca tgtctgtaat tgtggttgtc ttattactga cccttatgtt
     2701 gatagccatt ttttcggcaa ttgatcagct gggtatttca gaaatgggtc gcattgcaag
     2761 agatcttaca catttcatta tcaatagttt gcaagactga aaataa
//
LOCUS       DAZGVQ010000300         2765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000300 DAZGVQ010000000
VERSION     DAZGVQ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.303"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1221
                     /locus_tag="ACHLAG_005184"
     CDS             <1..1221
                     /locus_tag="ACHLAG_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HFP0658242.1"
                     /translation="PNTDFKDYIADEHDVIMLIHSAIESLSNKRHQYILLMRLGTDDS
                     PHSLADIAVKLGISRERIRQLQERALGLLAAKSRYEGTPGACLKALINSIGNSPYDIA
                     VWIYRIVHRDFVTSSELAAKFIIFTAGIPKARRDEIKNSLSLISQLQKQRLRERRAEL
                     AHAHAQERARIKKERVEFIFSKWLDNTHWPKYIAPPPPVEQLCSQRAVNDTDISGFFY
                     SQKLGRTVQYESGLEFKIMNLLECCDQVLFYQEQPFSIPYCFKNKSKKYYPDLLIATV
                     DGRCLLMEVKPTDRMTLSLTRIKSNAGRKWAHTRGWGWLVISDRFSLRQLEEHAISTN
                     TWKLIEAELKTSRILAWREMMELRTRYEIHRLDLIAYIIQSGAEVDNFYRIIPKISNN
                     TSNQRQPNESDCHN"
     gene            complement(1360..1512)
                     /locus_tag="ACHLAG_005185"
     CDS             complement(1360..1512)
                     /locus_tag="ACHLAG_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP0658243.1"
                     /translation="MPQRTFLTMLIVVCVTILCFVWMVRDSLCGFRVEQGNTVLVATL
                     AYEVKR"
     gene            1945..2148
                     /locus_tag="ACHLAG_005186"
     CDS             1945..2148
                     /locus_tag="ACHLAG_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HFP0658244.1"
                     /translation="MRTTVTIDDALYAQALDMAEPGMDKSDIFREAVKTFIRVQAAKR
                     LASLGGTAPDMEITSRRREDAVE"
     gene            2145..2519
                     /locus_tag="ACHLAG_005187"
     CDS             2145..2519
                     /locus_tag="ACHLAG_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFP0658245.1"
                     /translation="MILVDTSVWVDHFKNKNDTLIQLLLSDCVLMHPMILAEIACGTP
                     PAPRQQTLSNLDLLPKSHQATMTEVLAFIENEKLFGSGCGLVDITLLASTRITPGARL
                     WTLDKRLSRLADILNIGFQPVH"
     gene            complement(2608..2688)
                     /locus_tag="ACHLAG_005188"
                     /pseudo
     CDS             complement(2608..2688)
                     /locus_tag="ACHLAG_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein pndA"
BASE COUNT          845 a          615 c          582 g          723 t
ORIGIN      
        1 cccaatacag attttaaaga ttatatagcc gatgaacatg atgtcattat gctaattcat
       61 tctgctatcg aaagtttatc taataaacga catcaatata ttcttctaat gcgactcggc
      121 acagatgatt cacctcattc tctggcagat attgccgtca aattaggaat ttcccgtgaa
      181 aggataagac aacttcagga acgagccctt ggcttactag ctgctaaatc tcgctatgaa
      241 gggacacctg gagcttgcct taaagccctt attaactcaa taggtaattc accatatgat
      301 atagcagtat ggatttatcg tattgttcat cgggatttcg ttacatcctc tgagctagcc
      361 gccaaattca ttatttttac cgcaggcatt cctaaagcaa gacgagatga aataaaaaat
      421 tcgctctcat taatatcaca attacaaaag cagaggttac gtgaacgaag agcagaatta
      481 gcacacgctc acgctcagga aagagcccga atcaagaaag aaagagttga gttcatattc
      541 agtaaatggc tagacaatac acattggcca aaatatatcg cgcctcctcc ccccgtagaa
      601 cagctatgtt cacagagagc ggttaatgat actgacatat ctggattctt ttactctcaa
      661 aaactaggtc gtacagtcca gtacgaatca gggcttgagt ttaaaataat gaatctatta
      721 gaatgttgcg atcaggttct tttctatcaa gaacaaccat tttctattcc atattgtttt
      781 aaaaacaaat caaaaaaata ctatccagat ttactcattg cgacagttga tggtcgctgc
      841 ctacttatgg aagtgaagcc aacagaccga atgaccttga gcctcacgag aataaaaagt
      901 aatgcaggcc gcaaatgggc acatactcga ggatggggat ggttggtcat tagtgatagg
      961 ttctcgttac gtcagttgga agaacatgcc atctcaacaa acacatggaa acttatagag
     1021 gctgaactga aaacatcaag gattttagca tggcgtgaaa tgatggagtt gcgtacacga
     1081 tacgagattc atcgtttaga tcttattgca tatatcattc agtctggtgc tgaagtggat
     1141 aacttttatc gaattatccc gaaaatatcc aataatacat ctaaccagag acaaccaaat
     1201 gaaagcgact gtcacaattg atgacgtact ggacggaaac acattatagc tgccggacag
     1261 cagcaaccag caatcatccg gtaaaaaaga caaacagggc gcagtgaggt ctgccttcgc
     1321 aacaatccgg cgccagattt cctgccgccg tgttgcccgt taacgtttaa cttcgtaggc
     1381 taacgttgcc acaagcactg tgtttccctg ctcgacacgg aatccgcaaa gcgaatccct
     1441 caccatccag acaaaacaca gtatcgtcac acagacgaca attaacattg ttaaaaacgt
     1501 tcgttgtggc atcgcagccc ctccattgcc tttcggccgt catcgggcta ctataatgtt
     1561 gtcgatgcat tacagtagag ccctgggttg attgataaaa aaatcgcctg gggctttctg
     1621 ctgcatgccg tccccaaaac gctttcggca tacagcctca agcgcccgcc gctattctaa
     1681 tcgttttcct gtccgccaca atccttttca tgccgtgatg tttttggtat ccgggacagg
     1741 aaaaatgtcg tgaattatcc aggagtactg gcgatgaaca tgttcagttg caggctggtt
     1801 ttggtcattc tctataccat cctgttgttc agggcatcaa aaagtttctt ttccataaat
     1861 cagactccaa caacatttga cacgatgcat tatgtgatgc acatttgaat catataatgc
     1921 atcaaatgag aagagagaaa caccatgaga acaactgtaa caatcgacga tgctctgtac
     1981 gcacaggctc tggacatggc tgaacctggt atggataaat cagatatttt ccgtgaagca
     2041 gtgaaaacat ttatccgtgt acaggcagca aaacgtctgg catcactcgg cggaactgcg
     2101 ccagatatgg aaataacatc tcgcagacgt gaggacgcag tggaatgata ttggttgata
     2161 cgtctgtatg ggttgatcat tttaaaaata aaaatgatac attgattcaa ctattgctgt
     2221 cggattgtgt tcttatgcat ccaatgattc tggctgaaat cgcatgtggc acaccgccag
     2281 cacccagaca acagacatta agtaatctgg acttgttgcc gaaaagccat caggcaacaa
     2341 tgaccgaagt tttggcattt atcgaaaatg aaaaattatt tggttcaggt tgtgggctgg
     2401 tcgatataac actactggcg tcaacgcgaa ttactcccgg agccagacta tggacattag
     2461 ataaacgcct gtcacggctg gcagatattc ttaatattgg atttcagcca gtacattaac
     2521 cccgcaggga taataaagga aaaagggcgc agtgaggtct gccttcgcaa caatccggcg
     2581 gcagatttcc tgccgccgtg ttgcccgtta acgtttaact tcgtaggcta acgttgccac
     2641 aagcactgtg tttccctgct cgacacggaa tccgcaaagc gaatcccttg aggtagcctg
     2701 agtttaacgg acactccttc ctgaaataga atggcatcag aaggagctaa taatgagcag
     2761 aaaaa
//
LOCUS       DAZGVQ010000301         2730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000301 DAZGVQ010000000
VERSION     DAZGVQ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.304"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..378
                     /locus_tag="ACHLAG_005189"
     CDS             <1..378
                     /locus_tag="ACHLAG_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658246.1"
                     /translation="YALKLIRQLYPQVQWVQSFADERCGCLGVVYQASNFDYVGSHET
                     IFYELDGEWYHEICRNAIKRGGQRGEHLRANIDRASVHKFRQFRYIRFLNKRARKRLN
                     TKLFKVQPYPKPQTVKTGLKESE"
     gene            complement(508..2028)
                     /gene="bcsA"
                     /locus_tag="ACHLAG_005190"
                     /pseudo
     CDS             complement(508..2028)
                     /gene="bcsA"
                     /locus_tag="ACHLAG_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            complement(2025..>2730)
                     /gene="bcsQ"
                     /locus_tag="ACHLAG_005191"
     CDS             complement(2025..>2730)
                     /gene="bcsQ"
                     /locus_tag="ACHLAG_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFP0658247.1"
                     /translation="TITAALAWSLQMLGENVLVVDACPDNLLRLSFNVDFTHRQGWAR
                     AMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRLSDICSGLQQLKASGRY
                     QWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQALPDGAHILINDFRIG
                     SQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEYRSDALAAEEILTLANW
                     CLLNYSGLKTPVGSAS"
BASE COUNT          705 a          727 c          703 g          595 t
ORIGIN      
        1 tacgcgctca agctaatcag gcagctttat ccgcaggtac agtgggttca gtcgtttgca
       61 gatgagcgtt gtggctgtct tggtgtcgtg tatcaggcaa gcaattttga ttatgtaggc
      121 agccatgaaa caatattcta tgaacttgat ggcgagtggt atcacgagat ttgcaggaat
      181 gccattaaac gaggcggaca acgaggtgaa catctgaggg ctaatatcga cagagccagt
      241 gtgcataagt tccgacagtt tcgttacatc cggtttctga acaagagggc cagaaaacgc
      301 ctcaacacaa agctattcaa agtccagcct tacccaaaac cacaaacagt taaaactggt
      361 ttgaaagaga gtgaatgagc gcttaaaaac tcattctaaa actcaaaaga aaagcgccga
      421 tttagtatca aataagttgc gtatcggcgt ttttcagcag tatcaaatga aatgcaaatc
      481 ggtatcaaaa aaatcgccgc gctatacgag acggaagatt tgcaccatcc cgcgtgccca
      541 gcgaatacgc tgaccgatat gcgccgacag actttcggtc gccagccccg ccgcctgcgg
      601 aatacgcata tatgcagagg tgtagccacg acggtgcagg cgcagagacg tgtgcgcatc
      661 ttcggttacc gtttcgacag caataccgcc aatttcatcc aatggtttgc gacgaattac
      721 tgcacaggaa ccgcagaaga aagtggcgtc ccacatatcg ttgccatcct gcaccagacc
      781 atagaacagc gtgccttcgt tcggtgtttt acgaaaacgc cccaggttgc gttcaaacgg
      841 gtccggcgag aagaagtggt gtggagtctg catcatcgcc agctgtttct ctttcaggaa
      901 ccagcccatg gtcatttgca ggaacgatcg cgttggtacg tggtcgcagt cgaaaatcga
      961 cacgaactcg cctttggcat atttcagcgc attgttgatg ttgcccgctt tcgcatgttc
     1021 atgagtggta cgggcgatat atttcacccc cacgttttgc gcaaactggc gaaactcttc
     1081 cctgccgccg tcatcgagga tccagatgtt cagtttgtct ttcggccagt cgatacccag
     1141 cgaggcgtaa atggtatttt tcaccacgtt gagatcttcg ttgtaagtcg ggacaaagat
     1201 atccaccgac ggccacagcg acatatcttt cggcaatggc accggctgac gattcagcgg
     1261 ccatactacc tggaagtagc cgagcaccag cacaatccac gcgtacgttt cagcgaagag
     1321 cagaataagc ccgcacacca ggctgaccgg atcgtcccag ttcagcgtag aggtgtaacg
     1381 ccaccagata taacggcaag aaacggtcag cgacagcaca atcaacatta gcgccgagaa
     1441 gcgccccggc atccgccgta cgatcagcgc taccccccac agcagcatca ggaagataaa
     1501 ctgcgccagc gggttaaacg gctgagtaac gcagattaac gccagaatca gcgagaaggt
     1561 gacgatgata ccgaggatca accgccgcgc cccggcactc aaatgaccga gctctttttt
     1621 ctcatccaga tgctgtgttt tatggctaac gcgctcaggc agctcgttca tccattgatg
     1681 gtaacgtcca cgaatatttt gcagacctga aaatgcccgc ctgcgcggtt tcggcgtttc
     1741 tttgcgcgat gcaccgatca ataaccagca tgtttgaatg agataacgga ccgggtccag
     1801 cggacgcgga cgcgaggcgt tgatatgcgg atacaggttt ttatgttctg cgcgaatacg
     1861 ctgccagcgc gggtgctcca gcggaataaa aatccaggcc aggatcatcc agaaacagcc
     1921 gagcgtcgcg ctgaaagccg acgcaccgtg acgacgataa tcgcgataac gcccgataag
     1981 ccgcgcgttg accggcggga taagcaacca ccgggtcagg atactcatga tgcactcccg
     2041 actggcgttt tcagcccgga gtagttcaac aggcaccagt tcgccagcgt cagtatctct
     2101 tcagccgcca gcgcatcact gcgatattca cctactggtt gcttagccgc caggcattca
     2161 gccatcgctt catcacgatg aatgagcatc ggcagtaatc ggcgctggct ttgcaaccaa
     2221 agctggtaaa tatcgtcctg aacctgactg ccaatacgga agtcattaat caaaatatgt
     2281 gcgccatccg gcagcgcttg ctgatgcagt cggatatggc agttggcatc cacattgacg
     2341 attgccagcg agtgatcgca caaactcagc agctggtggg ttatctgcga ggcatcacgc
     2401 ggtaagtcga ttaaaatcca ctggtaacgc ccgctggctt ttagttgctg taagccggag
     2461 caaatatcgc tcagccgggt ttgccagtgc tgtggatttt cttgttcttc aatggataac
     2521 tgaccaaaag gcagcaaatc gagctgcgag gtgtagcgca acccagcgtc acgccagtcc
     2581 tggccatcca gcatcgctct ggcccagccc tgacggtggg taaaatcaac attaaatgac
     2641 aggcgcaaca agttgtccgg gcaggcatcg accaccagaa cattttctcc caacatttgt
     2701 aatgaccagg ctaatgcggc ggtgatggtt
//
LOCUS       DAZGVQ010000302         2562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000302 DAZGVQ010000000
VERSION     DAZGVQ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.305"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..303
                     /locus_tag="ACHLAG_005192"
     CDS             <1..303
                     /locus_tag="ACHLAG_005192"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658248.1"
                     /translation="LSAENTRMKTNKKDTKWYIFYRENSGEDILLEMSSFKECLSASK
                     ELMTPSNYMICIERNGERIKRWDREIIAGSNKWINCPPDNFEILGELITINRIIKK"
     gene            complement(293..2086)
                     /locus_tag="ACHLAG_005193"
     CDS             complement(293..2086)
                     /locus_tag="ACHLAG_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658249.1"
                     /translation="MEKVSIRKINRTEHFPSLNLPSDTSWVVWWYGFLKKNKRRGEQP
                     KVAITFREVQTDGTLCDRYYEQECLLTDLSFFKIGSVWTRGRCVAEILMEEKIFNVDF
                     SYANGGWKINSLYQAYKEGVSLPYDFSDYQLKSPYDKNWLLEFLTDDADKVIIHPLEF
                     FYRCYGHSDELKRVILTYSGNELRNRIFSEAYVEGDKIFYIKLRKRMNKYDAPFASFF
                     ALDGFTRKVIDRIQRQLDVCEDRKIYLKVAPWFKGKARIKVKGFWLENNVTFLGLSVT
                     GVSLPAGYTINYDRDNSNLVDEKAEQGSRQAWSNVPVRHIKNPDEPFSVDNAFSPDSG
                     ASTLEVEASVFEFLDDGCELSPLRRKKAKYISVTKNESDLHSLYSVNEVVDEQNSIST
                     GYMSLADKNEPERLYMESKGIVRDMWNAMVYLRGAYSERIKSLDWYTPESSFLNTQEP
                     ELVAIPEFNESEVENDKISKSILNWPYLDVVNKKDIRGFLISRMLIDDNMIYFMETQR
                     RVCNEETMNSFSEVEKFKGLVFTLDNECEIDKWVSLLAHESRFVKGILQKIVGKCPGT
                     AMTYKHSPAKNEPVACYSALLNALSKVGVTF"
     gene            complement(2241..>2562)
                     /locus_tag="ACHLAG_005194"
     CDS             complement(2241..>2562)
                     /locus_tag="ACHLAG_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658250.1"
                     /translation="DHFHIASFEGNRNPLWKKTSLEDLERAYPEFDFVIHKHHLEKWI
                     NGGLSPENVKKTIKEKGWSNKMLAARWGCTEVWVSKIINDENRKVQWNDAINGLPVIS
                     DNMI"
BASE COUNT          822 a          557 c          366 g          817 t
ORIGIN      
        1 ttatccgccg agaatacccg tatgaaaact aataagaaag acacaaaatg gtacattttt
       61 taccgtgaaa attcaggcga ggatattttg cttgaaatgt catcatttaa ggaatgtctt
      121 tcggcatcca aagaattaat gaccccatca aattacatga tatgcataga aagaaatggc
      181 gaaagaataa aacgttggga cagggaaatc atagcaggct ctaacaaatg gattaactgt
      241 ccacctgata acttcgaaat attaggtgag ctaataacca ttaatagaat tatcaaaaag
      301 taactccgac tttagacaat gcattaagca atgcgctata acaagcaacg ggttcatttt
      361 ttgcgggaga atgcttgtaa gtcatcgctg tgcccgggca ttttcccact atcttttgta
      421 aaataccttt aacaaatctt gattcgtgag ctaacaaaga tacccattta tcgatttcac
      481 attcgttatc aagtgtaaaa accagacctt taaatttttc cacttcagag aacgaattca
      541 tggtttcttc attgcacact ctcctctgtg tttccataaa ataaatcata ttatcatcaa
      601 tcaacatcct tgatatcaga aaacctctta tatctttctt attcacaaca tcaaggtaag
      661 gccagttaag aatcgactta gaaattttat cattttccac ttcgctttca ttgaactccg
      721 ggattgcaac aagttcaggt tcttgcgtat ttaagaatga gctttctggg gtataccagt
      781 ccagagactt aattctttca gaataagctc ccctcaaata aaccatagca ttccacatgt
      841 cacgaacaat cccttttgac tccatataca atctttcagg ttcattttta tcagctaatg
      901 acatatatcc tgtgcttatg ctattttgct catcaacaac ttcatttacc gaatataatg
      961 agtgtaaatc actttcattt tttgtaacgg atatatattt tgcttttttt ctccttaaag
     1021 gactcagttc acaaccatcg tcaagaaatt caaaaactga cgcttcaact tcaagagtcg
     1081 acgctccaga atcaggagaa aaagcgttat caacactgaa aggttcatcc ggatttttta
     1141 tatgccgaac tggaacatta ctccaggctt gcctgctccc ttgttcagct ttttcatcaa
     1201 ccaggttact gttatctctg tcataattta tagtatatcc tgcaggcaaa gaaacacctg
     1261 taacagaaag cccaaggaaa gtaacattat tttccaacca aaatccttta acttttattc
     1321 tggcctttcc tttaaaccag ggagccactt tgaggtatat tttacgatct tcacatacat
     1381 ctaattgacg ttgtattctg tcaatcactt tccgggtaaa cccatcaagg gcaaagaaag
     1441 aagcaaaagg agcatcatat ttattcattc tctttcttaa ttttatataa aagattttgt
     1501 ctccctccac gtatgcctca gaaaaaatcc tgttacgcag ttcatttcct gaataagtaa
     1561 gaataactct cttcaactca tcggaatgtc catagcaacg ataaaaaaac tcaagcggat
     1621 ggatgatcac cttatccgca tcatctgtta aaaactcaag gagccaattt ttatcatatg
     1681 gagatttcag ttgataatct gaaaaatcat aaggtaatga cacaccttct ttatatgcct
     1741 gataaagaga attaatcttc caccctccat ttgcataaga gaaatcaaca ttaaatattt
     1801 tttcctccat cagaatttca gcaacacacc ttcctcttgt ccagacactg cctattttaa
     1861 aaaaagacaa atcagtcaac agacattctt gctcataata tctatcacaa agagttccat
     1921 cggtctgaac ttcccgaaag gtaattgcta ctttaggttg ttcacctcgt cttttattct
     1981 tttttaagaa accataccac caaacaaccc aggatgtatc agaaggaaga tttaatgaag
     2041 gaaaatgctc tgtcctgttg atttttctta ttgatacttt ttccatacat ctgtccctct
     2101 aaacaacaga actcttatct cacaagcaaa tattgaagat aaaatagtta ttcacttaaa
     2161 ccagaaacgc tctgatgcga actacatcca gtatatatta ataatcttct tgccttatgc
     2221 tggtgaagca tgattacaca tcaaatcata ttatcactta tcacaggaag cccattaata
     2281 gcatcattcc actgaacctt tctattttca tcgttaatga ttttactcac ccatacctct
     2341 gtgcaccccc agcgcgcagc caacatcttg ttgctccagc ctttttcttt tattgtcttt
     2401 tttacatttt caggactaag tcctccatta atccattttt caagatgatg tttatgaata
     2461 acaaagtcaa actcaggata tgctctctct aaatcctcca gtgaagtttt cttccacagt
     2521 ggattcctgt ttccctcaaa agaggcgata tgaaagtgat ca
//
LOCUS       DAZGVQ010000303         2508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000303 DAZGVQ010000000
VERSION     DAZGVQ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.306"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            203..397
                     /locus_tag="ACHLAG_005195"
     CDS             203..397
                     /locus_tag="ACHLAG_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0658251.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
     gene            758..1051
                     /gene="iss"
                     /locus_tag="ACHLAG_005196"
     CDS             758..1051
                     /gene="iss"
                     /locus_tag="ACHLAG_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HFP0658252.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGFLGFITLGIYTPLEARVYCSQ"
     gene            complement(1142..1324)
                     /gene="rzoD"
                     /locus_tag="ACHLAG_005197"
     CDS             complement(1142..1324)
                     /gene="rzoD"
                     /locus_tag="ACHLAG_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFP0658253.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(1541..2038)
                     /gene="rrrD"
                     /locus_tag="ACHLAG_005198"
     CDS             complement(1541..2038)
                     /gene="rrrD"
                     /locus_tag="ACHLAG_005198"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFP0658254.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETMRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREIC
                     LWGQQ"
     gene            complement(2038..2253)
                     /gene="essD"
                     /locus_tag="ACHLAG_005199"
     CDS             complement(2038..2253)
                     /gene="essD"
                     /locus_tag="ACHLAG_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFP0658255.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT          699 a          609 c          548 g          652 t
ORIGIN      
        1 ccccgtaacc tgtcggatcg ccggaaagga cccgcaaaat gataataatt atcatctaca
       61 tgtcacaacg tgcatctacg ccatcaaacc acgtcaaata atcaattatg acgcaggtat
      121 cgtattaatt gatctgcatc aacttaacgt aaaaacaact tcagacaata caaatcagcg
      181 acactgaata cggggcaacc tcatgtcaac gaagaacaga acccgcagaa caacaacccg
      241 caacatccgc tttcctaacc aaatgattga acaaattaac atcgctcttg agcaaaaagg
      301 gtctgggaat ttctcagcct gggtcattga agcctgccgt cggagactaa cgtcagaaaa
      361 gagagcatat acatcaatcc aaagtgatga tggatgaaca tcccggtttc ttccaccatc
      421 gcaccggaaa agcgactatg agggtaaccc tgcgtctgtc agcacagtaa aacccggtgt
      481 gcatcgtttt tgattattcc cgcacactca cgcagaagga attccccgtc gggctacggt
      541 catggttaat gcgggaatac ggcgacgata cagcgcagct aaaagggtaa tggacagata
      601 gagcggttta tttcattcca caggattctg agtgcccccc ctcctccaat aggctgagca
      661 tccacctata tagttttaat tttcatcaat ccatttaact atcgtttaat tgttgtcaca
      721 taggattctg ccgtttttaa caatgcagga taataagatg aaaaaaatgt tgttttctgc
      781 cgctctggca atgcttatta caggatgtgc tcaacagacg tttactgttg gaaacaaacc
      841 gacagcagta acaccaaagg aaaccatcac ccatcatttc ttcgtttcgg gaattggaca
      901 ggagaaaact gttgatgcag ccaaaatttg tggcggcgca gaaaatgttg ttaaaacaga
      961 aacccagcaa acattcgtaa atggatttct cggttttatt actttaggca tttatactcc
     1021 gctggaagcg cgtgtgtatt gctcacaata attgcatgag ttgcccatcg atatgggcag
     1081 ctctatctgc actgctcatt aatatacttc tgggttcctt ccagttgttt ttgcatagtg
     1141 atcagcctct ctctgagggt gaaataatcc cgttcagcgg tgtctgccag tcggggggag
     1201 gctgcattat ccacgccgga ggcggtggtg gcttcacgca ctgactgaca gactgctttg
     1261 atgtgcaacc gacgacgacc agcggcaaca tcatcacgca gagcatcatt ttcagctttc
     1321 gcatcagcta actccttcgt gtattttgca ttgagcgcag caacatcacg ctgacgcatc
     1381 tgcatgtcag taattgccgc gttcgccagc ttcagttctc tggcattttt gtcgcgctgg
     1441 gctttgtagg taatggcgtt atcacggtaa tgattaacag cccatgacag gcagacgatg
     1501 atgcagataa ccagagcgga gataatcgcg gttactctgt tcattgctga ccccacaaac
     1561 agatttcacg ctcaatctca cgacgagtca tgagaccttt ccattgctta ccgccagcat
     1621 atgtccagcg acgtagctga tcacatgcgc ctttgatatc gccctggttt attttgcgaa
     1681 gaagcgtcga tgttctgaaa ttgccagcac ccacgttgta aacgaatgag taaagagcgc
     1741 cgcgcattgt ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgaccgtgg
     1801 caaggtcttt attcaggagg gctttgcatt ctgctttggt atacgtttta ccgagcatga
     1861 tgtcttttcc tgtatgcccg tgacatacag tccatacacc aacaatatct ttgtatggta
     1921 tgtagctgac accttccaga ccatcgttac cacttgggcc agtgattaac actgatgcta
     1981 tagcaattgc tccgccacca atagcagcag caacggcttt tcgtaatgat ggaggcatta
     2041 ttcacctctc gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta
     2101 cgtcagcagg ccaaatacca ggctacccag cacacctatt gctgcccact gtgagggcgt
     2161 gactttatcg agcagctgta aaaaccagta accggcacta cctgctgagg tgccataggc
     2221 gacacccgtt gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg
     2281 taaattgagg gaatacaacg tatcgcaaaa aagcagaaac gtaacagact cggagtcagt
     2341 gaataactca ggtattgggt tatcagctaa tatcgagact caaaaaatgg aaaaacccgc
     2401 tcgacggcgg gtttaagctg tgtgacgaag taaccactct taacagcata accaattttt
     2461 tacgtacgta aaccacaaaa tgatatttgc gagaatgcta ccgagtat
//
LOCUS       DAZGVQ010000304         2494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000304 DAZGVQ010000000
VERSION     DAZGVQ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.307"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..300)
                     /locus_tag="ACHLAG_005200"
     CDS             complement(<1..300)
                     /locus_tag="ACHLAG_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658256.1"
                     /translation="MSTQHKNRLPALVVSGYISAAPHKTGAGISTPLTNEAHSRASGF
                     FVRTVSPRLFRVRIMAGRTGPTSVGPVSSFAGCGNPVRLATLSFPPLVGEFSKFTN"
     gene            310..834
                     /locus_tag="ACHLAG_005201"
     CDS             310..834
                     /locus_tag="ACHLAG_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658257.1"
                     /translation="MLRINITWQLVIVCIRGVFLMSDKLPSDYDILFDLWYSYWLEHM
                     TSLLHRRVSAALRISAFLVGVAAFVLCDFVVLVLFSVVLFLLWQCMAASFERRAEKAE
                     WQAVSYQRLISQRHVISKECMIARLSFVEESDSPIMECMINLAWNKACEAVGSSSRNH
                     VSISGRLLSRLCGF"
     gene            complement(808..1158)
                     /locus_tag="ACHLAG_005202"
     CDS             complement(808..1158)
                     /locus_tag="ACHLAG_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP0658258.1"
                     /translation="MAVIYIAGPMTGHKNYNRSEFCVADLKLRCTGNIVLNPAVLPEG
                     LSQQQYMSICIPMLMCADAIYLLDGWEESAGARAEYAMALKLNIPVSFPEKRAAGETV
                     AQLLHKSKAAQATE"
     gene            complement(1162..1368)
                     /locus_tag="ACHLAG_005203"
     CDS             complement(1162..1368)
                     /locus_tag="ACHLAG_005203"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658259.1"
                     /translation="MIKESTVIRFTTHGHQYEVDESLIDQGMTRRDSRNPDIYHIRLI
                     NGSSFTATNVEEVRVLTATRDTGR"
     gene            complement(1365..1601)
                     /locus_tag="ACHLAG_005204"
     CDS             complement(1365..1601)
                     /locus_tag="ACHLAG_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658260.1"
                     /translation="MNSENNKELLRGLRLIAHAHEETARAAAAAGRPKVVTSSAISLR
                     ITMEAIQVIQDQEREIAALKKLLHGKSAGQGVKQ"
     gene            complement(1637..>2494)
                     /locus_tag="ACHLAG_005205"
     CDS             complement(1637..>2494)
                     /locus_tag="ACHLAG_005205"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HFP0658261.1"
                     /translation="GASVFMNTSYKRYILGDSNGALINFYREAKSNTENLINEAATLF
                     LNHNRGGYLAIREAFNRENHALLCSRARISNSRKLRLAAMFLYLNRHCFNGLFRVNGK
                     GDFNVPCGSCRKHYFPYQEIRAFAEKANSTETWLIHGDFRSTLRAARFFFAKGHTLCV
                     YCDPPYLRFSGKDNFTAYGKPFDEHEHVRLRAALDRLSQETGGMTSITISNSDTPETR
                     RIYRGYRMNCIQAPRSVGARTREPAPEVIATLRQCDACGHYGGGYCPDCGPVTGDATY
                     SVMFTPAST"
BASE COUNT          504 a          620 c          687 g          683 t
ORIGIN      
        1 gtttgtgaat tttgaaaact caccaaccag aggtggaaaa ctcagggtgg cgagacgtac
       61 agggtttcca caaccggcaa acgaagaaac cggcccgacc gaagtcggcc ctgtacgccc
      121 cgccataatt ctgacgcgaa aaagacgtgg cgatacagta cgcacaaaaa aaccgctggc
      181 gcggctgtgc gcttcgtttg tcagcggggt ggaaatcccg gcacccgttt tatgaggtgc
      241 agcggaaatg taacccgaca cgacgagcgc tggcaagcgg tttttgtgtt gcgtgctgat
      301 gtggttagaa tgttgcgaat taatatcacc tggcaactgg tgattgtctg catacgcggg
      361 gtgtttctga tgagtgataa gctcccttct gattacgaca ttctttttga tctgtggtac
      421 agctactggc ttgagcacat gacaagcctt ctccaccgtc gtgtttctgc cgctctgcgg
      481 atcagcgcct ttctggttgg tgttgcggcg tttgtcctgt gtgattttgt tgtgttggta
      541 ctcttcagtg tggttctgtt tcttctctgg cagtgcatgg ctgccagttt tgaacgcagg
      601 gctgaaaagg ctgaatggca ggcagtcagt taccagcgcc ttatcagtca gcgccatgtg
      661 attagtaagg aatgcatgat tgcgcgcctc tcctttgttg aggaaagtga cagtcccatc
      721 atggagtgca tgattaatct tgcctggaat aaagcctgtg aggcggttgg cagctcttcg
      781 cgcaatcatg tttcgatctc cggtcggtta ctcagtcgcc tgtgcggctt ttgatttgtg
      841 aagcagctgc gcgacggttt cgccggctgc ccgcttttcc gggaatgaca ccggtatgtt
      901 cagcttgagt gccattgcgt actcagcccg tgcgccagcg gactcttccc atccatccag
      961 taagtaaatg gcatcggcac acatgagcat cggaatacaa atactcatgt actgctgctg
     1021 gcttaatcct tcaggcagta cggcaggatt aagaacgatg tttccggtgc acctaagctt
     1081 caggtccgcc acgcaaaact ccgatctgtt gtagtttttg tgtccggtca tgggaccggc
     1141 gatataaatc acggccatgc tttacctccc cgtatcgcgc gttgctgtca gcacccgtac
     1201 ttcttccacg ttcgtggcgg tgaaagatga accgttgata aggcggatgt gatatatatc
     1261 gggattacgt gaatcccggc gtgtcatgcc ctgatcaatc aggctttcgt caacttcgta
     1321 ctgatggccg tgggttgtga atctgataac ggttgactcc ttgatcattg cttgactcct
     1381 tgcccggcag atttaccgtg gagtagcttt ttaagggcgg cgatttctct ttcctgatcc
     1441 tgaattacct ggattgcttc catggtgatc ctcagtgaaa tcgctgaaga ggtaacgacc
     1501 tttggtcttc cagcggctgc ggcagctctt gctgtttcct cgtgtgcatg ggcgatgaga
     1561 cgcaatcccc gcaaaagctc tttgttgttt tcgctattca tttcctgact ccggtgttac
     1621 tgattcatct gtttattcac gtactggcag gggtaaacat cacgctgtaa gttgcatctc
     1681 ccgtcacagg tccgcagtcc gggcagtagc caccgccata gtgaccgcag gcgtcgcact
     1741 gtctcagggt ggcgataact tccggtgctg gttcgcgggt tcttgctcca acgcttcgtg
     1801 gtgcctggat gcagttcatc cggtatcccc gataaatgcg ccgtgtttcc ggtgtgtcgg
     1861 aattggagat ggtgatgctg gtcatgccgc ccgtttcctg tgaaagacgg tcaagtgccg
     1921 ctctcaggcg aacgtgctcg tgttcatcga atggttttcc gtaggcggtg aaattgtcct
     1981 tgccactgaa tcgcaggtaa gggggatcgc agtacacgca aagcgtgtgg ccttttgcaa
     2041 agaagaatct ggcagcgcgg agagtgctac ggaaatcacc gtgaataagc cacgtctctg
     2101 tactgtttgc tttttctgca aatgcgcgga tttcctggta agggaagtaa tgcttgcggc
     2161 agctgccgca aggcacgtta aaatcacctt ttccgttcac gcggaacagg ccgttaaaac
     2221 agtgtcggtt caggtacaaa aacatggcgg ccagtcgcag tttacgactg ttgcttattc
     2281 gtgctctgct gcacagcagg gcatgattct ctctgttaaa ggcttcgcgg atggcgaggt
     2341 acccccctct gttgtggttc agaaacaggg tggccgcctc gttgatcaga ttttcggtgt
     2401 tacttttcgc ttcccggtag aaattaatca gcgcaccatt ggaatcaccc aggatgtagc
     2461 gcttgtaact ggtgttcatg aataccgatg cgcc
//
LOCUS       DAZGVQ010000305         2486 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000305 DAZGVQ010000000
VERSION     DAZGVQ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.308"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            296..490
                     /locus_tag="ACHLAG_005206"
     CDS             296..490
                     /locus_tag="ACHLAG_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0658262.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDSWRRQIDKRAAEKI
                     SASQSAGAKTEAPLIPEQPS"
     gene            516..785
                     /locus_tag="ACHLAG_005207"
     CDS             516..785
                     /locus_tag="ACHLAG_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP0658263.1"
                     /translation="MSEIKSLVTAEAVKEVLRSEEVRSALKQQLRQNLEARLDAEVDA
                     ILDELLGAPTVPEPEGIADDSAVSDGVGSQPDGSEEPQPDGEMMM"
     gene            861..1076
                     /locus_tag="ACHLAG_005208"
     CDS             861..1076
                     /locus_tag="ACHLAG_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP0658264.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGD"
     gene            1080..1565
                     /locus_tag="ACHLAG_005209"
     CDS             1080..1565
                     /locus_tag="ACHLAG_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658265.1"
                     /translation="MNHEEMNQRFSRLENEIAELNKKLSVLMPSEDEKKRRDEQFAAF
                     YDYCRKVMSRNLAECFRIQKNRERSANSEILDNFLKGTHLASDCFIAHSFGGFHEAIK
                     HDVERSPGILCSIPVSIEIDTNTITGELVTAGVGIFRHEGNDAKGSFTKIGPFFLKVE
                     R"
     gene            2077..>2486
                     /locus_tag="ACHLAG_005210"
     CDS             2077..>2486
                     /locus_tag="ACHLAG_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0658266.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVVTGMKLSKKKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGAC"
BASE COUNT          675 a          542 c          678 g          591 t
ORIGIN      
        1 cagagcagac ggcatttgac cttgtgatgt ggacaaatga tggctttaca gcagatacgc
       61 tgacgctgac agatatcacg gggacaaaag cgacgtatcc ggtactgctt gatacggtca
      121 ctgtcaacgt taacgaaagc cgggcatcag cataaccggc aaaaaaaaac cgccagcggc
      181 aggaacggaa gctggcggag gtaatcccaa tggagaatgt aaagaaaaga tgctttcgta
      241 cattggtttt ttaaatgaaa acagttctca ttgtcaacca taacggtaag aaactatgac
      301 atttattcat caggtgatgc tgtacttctg tacggcggtc tgtgtgctgt atcttctttc
      361 gggtgggtac agggcagtgc gcgattcctg gcgcaggcag attgataaaa gggccgcaga
      421 gaaaatcagc gccagtcagt cagccggagc aaaaacagaa gccccactca ttccggaaca
      481 accttcttaa taaccccttt caacgagaaa atcctatgtc agaaataaaa tcgctggtca
      541 ctgctgaggc agtgaaggaa gtcctgcgct ctgaagaagt cagaagcgca ctgaaacagc
      601 aacttcggca gaaccttgag gcgcgtcttg atgcagaagt ggatgccatt ctggatgaac
      661 tgctgggcgc accgactgtt ccggagccgg aaggcattgc ggatgacagt gctgtttcag
      721 atggcgtcgg gtctcagcct gacggtagtg aagagcctca gcctgacggc gaaatgatga
      781 tgtaaccatg cgcagtgggt gtcggtgtga gctgatgccc cacttgttgt tgtgagcttc
      841 cggattgcgg gagacggggt atgtaccaga tggaaaaaat cacaacaggt gtgtcataca
      901 ccacgtcagc ggtgggaacg ggctactggt tcctgcagtt gctggacagg gtttccccgt
      961 ctcagtgggc ggcaataggc gtgctgggga gtctgctgtt tggtctgctg acatatctga
     1021 cgaacctgta tttcaaaatt aaagaagacc ggcgtaaggc ggcacgggga gattaggtga
     1081 tgaaccatga agaaatgaat cagcgcttca gtcgtctgga aaatgaaatt gctgaactga
     1141 ataaaaagct gtcggtgctg atgccttctg aagatgaaaa aaaacgccgc gatgagcagt
     1201 ttgctgcgtt ttacgattat tgccggaaag tgatgagcag aaatctcgca gagtgtttcc
     1261 gtattcaaaa aaacagagag cgctctgcaa atagtgaaat tctggataat tttttaaagg
     1321 ggacgcatct cgccagtgat tgtttcattg ctcattcttt tggtggcttt catgaggcca
     1381 tcaaacatga tgttgagaga agtccaggta tcctctgttc cattcccgta agcatcgaaa
     1441 tagacacaaa tacgataacc ggtgaactcg ttactgccgg agtcggtata ttccgtcatg
     1501 aggggaacga tgccaaaggt tcctttacaa aaatcgggcc gttctttctg aaggtagagc
     1561 gatagtttgt taagtaccca tccgacagct cgctcatttt ggtttatttt cgtatcgcga
     1621 aacaaaaatt cgaagcggcc agatgacgca tgggtgcaac caaataccgg ggcgtattca
     1681 tcaacctttg gtgttcctga aaacatgcag tccgtgctct cgaatgcaga tgaattgctg
     1741 ttgatccact taacaaaaga aaaaaaggtc tgaatcgctt gtctgtctga atattcaaca
     1801 aaatcctcaa ggtgtgtctc gatcagttcc gggtgtttac gaaagtcata atattgaccg
     1861 ggacgatatt tatgagtggt tgttttatcc catggttgtg ctctgcgtcc ctgtaaacgg
     1921 aggtctgccg ctggatagat aagcataagt aatctcctgg tggaatgtaa tccacgatca
     1981 tccggggaaa ttaagaaccc gccagtgccc accactggtg gggtgaaggc ttaacatatc
     2041 cagggattcg gaaccgataa atcctgataa atagccatga acaccaaaat caaatacggc
     2101 ctgtcggctg ccgttctggc gctgattgcc gcaggggcgc ctgcgcctga tattctcgac
     2161 cagtttctgg atgaaaaaga aggtaaccac accacggcat accgtgatgg tgcggggatc
     2221 tggaccattt gtcgtggtgc caccagggtg gatggcaaac ctgtcgtcac gggcatgaag
     2281 ttgtcgaaga aaaaatgcga ccaggttaac gccattgagc gtgataaggc gctggcatgg
     2341 gtggagaaaa acatccgggt gccactgact gaaccccaga aagcggggat cgcgtcattc
     2401 tgcccgtata acattggccc cggtaagtgt ttcccgtcga cgttttacag acgaattaat
     2461 gcaggtgatc gcaggggagc atgcga
//
LOCUS       DAZGVQ010000306         2469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000306 DAZGVQ010000000
VERSION     DAZGVQ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.309"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..492
                     /locus_tag="ACHLAG_005211"
     CDS             <1..492
                     /locus_tag="ACHLAG_005211"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0658267.1"
                     /translation="APQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVADPNL
                     DELYQVYSQPGHAYWVVEFDGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGKGLAK
                     KLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHINYALGCTGHVDCEVRML
                     REL"
     gene            complement(538..954)
                     /gene="rraB"
                     /locus_tag="ACHLAG_005212"
     CDS             complement(538..954)
                     /gene="rraB"
                     /locus_tag="ACHLAG_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFP0658268.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1116..2120
                     /gene="argF"
                     /locus_tag="ACHLAG_005213"
     CDS             1116..2120
                     /gene="argF"
                     /locus_tag="ACHLAG_005213"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP0658269.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            complement(2221..>2469)
                     /locus_tag="ACHLAG_005214"
     CDS             complement(2221..>2469)
                     /locus_tag="ACHLAG_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658270.1"
                     /translation="FLKDFFMKQPDFAKWYFYQLLKDYEGEQLYLNELGYVYGNEEKT
                     NEIVKSNPGYVVKIFEEKMVNELKIRTRMMKILRKIYV"
BASE COUNT          640 a          591 c          582 g          656 t
ORIGIN      
        1 gcgccgcaat cacctgtaat gcgtcgcctg acgcagcagg ataatcccgc tatcgcccgc
       61 gtcattcgtc aggtatccgc cgaatacggt cttaccgctg ataaaggcta caccgtcgcc
      121 gatccgaacc ttgacgagtt gtatcaagtg tatagtcagc ctggccatgc atattgggtg
      181 gttgagttcg atggtgaagt ggtcggcggc ggtgggattg cgccattaac cgggagtgaa
      241 tcggacattt gcgaactgca aaagatgtat tttcttccgg ctattcgcgg taaagggctg
      301 gcgaaaaaac tggccttaat ggcgatggag caggcacgag agatgggctt caaacgctgc
      361 tatctggaaa cgaccgcttt tttaaaggaa gccattgcgc tttatgagca tttgggcttt
      421 gagcatatca actatgcgct tggctgcacg ggccatgtcg attgtgaagt gcggatgcta
      481 cgcgaactct aaaaaaatgc ccgctagtgt gtgctatcgg gcattttaat caattaatta
      541 gtggcgaaca ccgtcatcgt cttcatcgac aaaatcttca tcgtcgccgt cttcgccatt
      601 gggatcttca aagtaagtgc cccaaccgtc gtactcaacg tcaaatttct ccgccagcgt
      661 catcagttgt tcaacctggg catcgatcag atcggcattc aacgcgcact cgctgaggat
      721 gtcgcagcaa atcacgatat caccatcttc aacttccagc tcttctggat cggtcacttc
      781 gtaaccgagt ttaaacgctt caactgctgc tttttccagg gtttccagat cgtctgcgga
      841 aagatggtgt tcgatggtgt acagcgcgtc cggatcgctg ccatcttcca gtaattcttc
      901 aataatcaaa cgtgtttctt cacgctgttc ttccagttgt tccgggtttg ccatggctca
      961 ttcctcttaa tgtcctgccg atactcttat tgtcacacac cactgacatt gcctccacct
     1021 ttgtgacaaa gatttatgct ttagacttgc aaatgaataa tcatccatat aaattgaatt
     1081 ttaattcatt aaggcgttaa ccacaggagg gatctatgtc cgggttttat cataagcatt
     1141 tcctgaaatt actcgatttc acgccagctg aactcaacag cctgctgcag ttagccgcga
     1201 agctgaaagc cgataagaaa agcggcaaag aagaagccaa actcactggt aaaaacatcg
     1261 cgctcatctt cgaaaaagac tcgacccgta cccgatgctc tttcgaagtt gccgcatatg
     1321 accagggtgc tcgcgttact tatctcggcc caagcggcag tcagattggt cataaagagt
     1381 cgattaaaga cactgcccgc gtgctgggtc gcatgtatga cggtattcag tatcgcggct
     1441 atggtcagga gattgtcgaa acactggcgg aatacgctgg cgtgccggta tggaacggcc
     1501 tgaccaatga gtttcacccc acgcagttgc tggcggatct cctcaccatg caggagcatt
     1561 tacccggtaa agcattcaac gaaatgacgc tggtctatgc aggtgacgca cgtaacaaca
     1621 tgggcaattc gatgctcgaa gctgcggcgc ttaccggtct ggatttgcgt ctggtcgcgc
     1681 cacaggcatg ctggccggaa gctgcgctgg ttacggaatg ccgcgccctg gcacagcaaa
     1741 atggcgggaa tattacgctg acggaagatg tagccaaagg agttgaaggt gctgacttta
     1801 tctataccga tgtatgggtg tcgatggggg aagcaaaaga gaaatgggca gaacggattg
     1861 cattactgcg tgattatcag gtgaacagca agatgatgca gttgaccggt aacccggagg
     1921 tcaaattcct ccactgcctg cccgcgtttc atgacgacca gacgacgctt ggtaagaaaa
     1981 tggcggaaga gtttggccta catggcggaa tggaagtgac tgatgaggtc ttcgaatctg
     2041 ccgccagcat tgtttttgat caggcggaaa accgcatgca taccatcaaa gcggtgatgg
     2101 tcgcgacgct cagtaaataa ttgatatcgc tataaagatg ccgggaaata caaaaacatc
     2161 ccggcatcac attattttat gcagtaatag ttcaattatt ccatccatat ttttctattg
     2221 ctatacgtaa atcttccgga ggattttcat cattcttgtt ctaattttta attcattaac
     2281 catcttttct tcaaaaatct taacgacata acccggattg ctttttacaa tttcatttgt
     2341 tttttcttca ttaccataaa catatcctaa ctcattaaga tacagctgtt cgccctcata
     2401 atctttaagt aattgataga aataccactt agcaaaatca ggttgcttca tgaaaaaatc
     2461 cttcaaaaa
//
LOCUS       DAZGVQ010000307         2461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000307 DAZGVQ010000000
VERSION     DAZGVQ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.310"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1239..1784
                     /locus_tag="ACHLAG_005215"
     CDS             1239..1784
                     /locus_tag="ACHLAG_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HFP0658271.1"
                     /translation="MSKSKALNINKRFSENPFIKDLIIPVKNKQVRISRLGEDNNIMI
                     NQSTGEVLGGTSVVTYKKVDSDNFIKLFTQNIKLAFELTQAGQKALFVLFWAVQNKTN
                     NDLVLLDQYTLNDFINENKGLSMSLPTFKRGLGELVKSQILAMNKRKGFYYINPNFVF
                     NGDRVAFTTVLEMNKSSHKDK"
     gene            complement(1864..2289)
                     /gene="mobA"
                     /locus_tag="ACHLAG_005216"
     CDS             complement(1864..2289)
                     /gene="mobA"
                     /locus_tag="ACHLAG_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFP0658272.1"
                     /translation="MAKNKRSGSEVRQRTKVITLRVNNRVASEIRRRAKNEGLTISEY
                     IRTASLNNEIKQRVPSRYLDELIRLGRMQKKLFDKGKRPKDKEYLEVMHKIILLCDEM
                     KIVTKRISDVYNEMDLLKQEIKIIKRHHKNKYPGSNIFS"
BASE COUNT          732 a          500 c          492 g          737 t
ORIGIN      
        1 aaaaaaacga tgcttcattc aatattattt acacagattt taaaagttat tgtttgttat
       61 ttaataaata acgcaaaatc aattgaaagt tttgttcttt gtcgtttgtt gattattgag
      121 taaataaaga tcgtgtcttc ctgattatat aatccattgc ctgttagtga tacgtagtgt
      181 gtccaaggaa caggaagcgg aagagcaaga tgtagcatgt gcataaaata taaatatgtg
      241 agttattgat atcgcattta tatattaaaa atataactta acgtcgctct taatatttat
      301 ttcatcctgg caatgggagc attaatcccg tgcagcgctc cgccagctgc tgacggcctc
      361 gacatgcaac cttcaatccg ataaaagatc ggggcgcgtt tccggcaaca actaaccata
      421 gggatggttt gcttgccgac aaacgccgga aacgcgcctc aaatccacca agccgccacg
      481 gttccggctg cgccttgagc gcaaccggaa accgactggg gagaacccca gacccccacc
      541 gctgcaacat catcggtctg cgaccaccta ccaaccgagc ggctgcctgt accgtgcaaa
      601 ggggctttgc aggggctggc gcggttccta gcgacctgaa aacccctggt taggggtatc
      661 ggcgcgcctg gcctcctgaa accctctgta agcgtttttc gcgccttcca tgccctggcg
      721 ggcatttaaa acccgtttcg tgcgtctgag agcttctgag agcgttttag gggctatctg
      781 gcaactcatc cccgaaagag caaccgcgga atcaccgaca acatgcgaga ggtaattccg
      841 ctcgccgcaa gtcgatgacc gagcgtagcg agcgaatcga cgaggaagcg gaagagaacc
      901 ggaagctaca ttgagcactt acgcacagaa tttggcctac gcccccgcgt ccgtgcacgc
      961 gtttaataag aattaagaag ggaaatcgcg ataaaccgcg ccaatcctag aatctaatga
     1021 tcaggatatt gatctaacgt atcagaatag agcatctaac atatcaatta gttgatattt
     1081 aaagtattga ttgtttgttt tttgctcatc gcgagtgtga tttatttgat ctttaaggta
     1141 tcaaaactat gatactagtc ggatcggttt attgacattt gtagattttt tcttgatagt
     1201 cgaggtatca gtttattgat acctgagagt gtaaaataat gagcaagagc aaggctttaa
     1261 acataaacaa acgatttagt gaaaatcctt tcatcaaaga tttgataatt cctgtaaaaa
     1321 acaaacaagt aagaatatcg cgattaggag aagataataa cataatgatt aaccaatcaa
     1381 ctggagaggt tttaggggga acctcggtag taacttataa aaaagttgat agtgataatt
     1441 ttattaaatt gtttacccaa aatataaaat tggctttcga gttaacacaa gctggtcaaa
     1501 aagcactatt tgttttattt tgggcagtac aaaataaaac aaataatgat ctagtcttat
     1561 tagatcaata cacgctaaat gattttataa atgaaaacaa aggattaagc atgtcccttc
     1621 caacattcaa acgtgggctt ggggagttgg taaaatcaca aatattagca atgaataagc
     1681 gaaagggatt ttattatata aatcctaatt ttgtttttaa tggcgataga gttgcattca
     1741 caacagttct tgaaatgaat aaatcgagcc ataaagacaa atgacaatgt attaagcccg
     1801 tcaaaggaaa atttcaaatc tattggcggg ttttaatatt cgtaacggct agcgccgaag
     1861 attttagcta aatatatttg agccagggta tttattttta tgatgccgtt ttattatttt
     1921 tatttcctgt tttaatagat ccatttcatt atatacatca gaaattcgct tcgttactat
     1981 tttcatttca tcgcaaagta aaattatttt atgcatcacc tcgaggtact ccttatcctt
     2041 cggccgcttt cccttatcaa ataatttctt ctgcatccga ccaagtcgga ttaattcatc
     2101 taaatacctg gaaggtacgc gctgttttat ttcattattt aaacttgccg tcctgatata
     2161 ttctgaaatt gtcagtcctt cgttcttcgc tcgccgtctg atttcgcttg caactctgtt
     2221 atttactcta agggtgataa ccttagttct ctgccgtact tctgaaccac tacgtttatt
     2281 tttagccatc gcaatgactc tccattttgc acgtttgggg tgtcggggct tgccctgaca
     2341 ggaggtattc tcatgcaagc gaatatattc gcggtatgac gaatacacat cctgtcccat
     2401 tttccgctac aacttttcga cgaaaataaa ataccgcgtc gtaacctggt atttctgata
     2461 c
//
LOCUS       DAZGVQ010000308         2364 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000308 DAZGVQ010000000
VERSION     DAZGVQ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.311"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            16..>2364
                     /locus_tag="ACHLAG_005217"
     CDS             16..>2364
                     /locus_tag="ACHLAG_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP0658273.1"
                     /translation="MSDHNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RIDARLDEETVDFLTNTNEGKRINSHLHHDEERRIRLEKLARYKRENPPTLVSEAMAE
                     LRTLPSFLGNALIRDLARLNRKQQNARNEGVKNNNTHVADNFVRRGLSRRLNRIKQVN
                     ERFATTAFKATAARHRLDELLMLPELNRDQIQRLATLTAAAFSTELERICDEVIERTG
                     KGDDNLFTWLLTYQQLARMALKLGVNPPYWPSLEIRRDRRTAPDPELVPGAVMRITCA
                     TWWNNQLRHLADLWREELLRAAGRVSRKASPYISHESLQEFREKRQRTRDFLKSWDIE
                     NEDGERLSLEDVYWSGLGNPRNRRNEMMACVRGMEQVAESRGDSAFFVTVTCPSRFHS
                     VNDDGSLNPKYNGATVRDASDYLVYDVFAAARKKLNKEGLSWYGVRTVEPHHDGTPHW
                     HMLVFTAPDNEERIIEIMRDAAIKSDRAELGDDISPRFKCEKIDPAKGTPASYIATYI
                     GKNLDASAFHDNDPKTGKPYADEESGKTMAETVENAIAWASLHRIRQFQFFGIPPRQV
                     WRELRRLAGQMERNPASPKRLDHDDIDAILAAADVGCFATYINRQGGVLIPRNLYLVR
                     TAYETADEANDYGEFPQRIYGICAPSLGERYTICTHPDEWKLVRKEAKADNSTDEGFD
                     VECGFAAPWTRGNNCPHDEKTDTGEGKTPPPETRKEQKLTIPEGDLSEWLRSLTPTER
                     KQLTRQLKDAPSDTKTVKTGGPTTPDDVIHVRTALPLTDVTTT"
BASE COUNT          637 a          703 c          622 g          402 t
ORIGIN      
        1 cggggagtgc gacgcatgag cgaccataac gcacaactgg cgtacccgtg gaacgccccg
       61 ctaccggtta tcgatcctga aacgttcggc agagcacaac caaccgcatt acgccaggcc
      121 attcagagct acatcaacga ggacattcgc attgatgcca gactggatga agaaaccgtc
      181 gacttcctga caaacacaaa cgaaggcaag cgaattaatt cgcatcttca ccacgacgaa
      241 gagcgtcgga tcagactgga aaaactggca cggtacaagc gcgagaatcc gccaacgctg
      301 gtatcggaag ccatggcaga acttcgcacc ctgccctcgt ttcttggcaa cgcgctcatt
      361 cgtgatctgg cgcgtttaaa cagaaaacaa cagaacgccc gcaatgaagg cgtgaaaaac
      421 aacaatacac acgtagcaga taacttcgtt cgccgtgggc taagcagacg cctcaaccgc
      481 atcaaacagg tgaatgaacg cttcgccacg acagcattca aagccacagc agcacgtcat
      541 cgtcttgatg aattactcat gctgccggaa ctcaaccgtg atcagattca gcgacttgcc
      601 acactgacgg cggcggcatt ttccaccgaa ctggaacgca tatgcgatga agtcattgag
      661 cggaccggaa aaggtgacga taacctgttc acctggttgc tgacttacca gcagctggcg
      721 cgcatggcgt taaaactggg cgtgaaccca ccatactggc cgtcactcga gatcagacgt
      781 gaccgccgca cggcaccaga ccctgaactg gtaccgggtg cggtaatgcg tatcacctgc
      841 gccacctggt ggaacaacca gctgcgccac ctcgctgatc tctggcggga ggaattactg
      901 cgtgccgccg gaagagtatc gcgcaaggca tccccctaca tcagccatga gtcactccag
      961 gaattccggg agaaacgcca gcgcacccgt gattttctca aaagctggga cattgaaaac
     1021 gaagacggtg aacgcctgag tctggaggac gtgtactggt cagggctggg gaatccgcgc
     1081 aaccgccgca acgagatgat ggcctgcgtc agagggatgg aacaggtcgc cgaatcgcgt
     1141 ggtgactccg cgttcttcgt gacagtgacc tgcccttccc gctttcacag cgtgaacgat
     1201 gacggcagcc tgaacccgaa atacaacggt gccaccgtgc gcgatgccag tgattatctg
     1261 gtgtacgacg tctttgcagc tgcaagaaaa aaactcaaca aagaaggcct gagctggtac
     1321 ggggtgcgca ccgttgagcc acaccatgac ggcacaccgc actggcacat gctggtattc
     1381 accgccccgg acaatgaaga acgcatcatt gaaatcatgc gtgacgcggc aatcaaatca
     1441 gatcgcgcag aactggggga tgatatttct ccgcgcttca agtgcgaaaa aatcgacccg
     1501 gcaaaaggca caccggcaag ttacatcgca acctatatcg gcaagaacct ggacgccagc
     1561 gcctttcacg ataatgaccc gaaaaccggc aaaccttacg ccgatgagga atcagggaaa
     1621 accatggccg aaaccgtgga aaacgccatt gcctgggcga gtctccatcg catccgtcag
     1681 ttccagttct tcggcatccc gccccgccag gtgtggcgtg aactacgccg ccttgccgga
     1741 caaatggagc gcaacccggc atcaccgaag cgcctggatc atgacgacat tgacgccatt
     1801 ctggccgctg ctgacgtggg gtgttttgcc acctacatca accgtcaggg cggcgtgctc
     1861 attccgcgca atctgtatct ggtccgcacc gcatacgaaa ccgcagatga ggccaacgac
     1921 tacggcgaat tcccgcagcg catctacgga atttgcgccc cgtcgctcgg cgagcgctac
     1981 accatctgta cccatccgga cgaatggaaa ctggtcagaa aagaagcgaa agccgacaac
     2041 agcacggacg agggttttga cgttgagtgc ggctttgccg caccttggac tcgtggcaat
     2101 aactgtcccc atgatgaaaa aacagacacc ggagagggta aaaccccacc accggaaacc
     2161 aggaaagaac aaaaactgac catcccggaa ggtgatttga gcgaatggtt aaggtcactg
     2221 acccccacag agagaaaaca actcaccagg cagctgaaag atgcgccatc tgacacaaaa
     2281 accgtgaaaa ccggcggccc caccacgccc gatgacgtca tacatgtgcg tacagcattg
     2341 ccgctgactg acgtcaccac cacc
//
LOCUS       DAZGVQ010000309         2352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000309 DAZGVQ010000000
VERSION     DAZGVQ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.312"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            159..2068
                     /locus_tag="ACHLAG_005218"
                     /pseudo
     CDS             159..2068
                     /locus_tag="ACHLAG_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001470092.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            2101..>2352
                     /locus_tag="ACHLAG_005219"
     CDS             2101..>2352
                     /locus_tag="ACHLAG_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658274.1"
                     /translation="MLEHFGAEASVLDMTIIVRSNPSKAAILEEFLHGTQEKLGIAEK
                     LGRYGLGSAETHVKDFMIRHKKMLGLSDEDVAILKILKDK"
BASE COUNT          583 a          592 c          731 g          446 t
ORIGIN      
        1 gttctgattt agttttgtcg agttctttga caatagcgat aaagatttat atgcgtttaa
       61 atcctaaaat gaaaatattg gattaatctg tgtcgttcag actctatatt gagttattgc
      121 gaaccgggtg ttgcaggtgc acccttccgt gaccctgcaa ccgccacggc aacctgctgg
      181 cctggacgga cggcaacggt gttgtctgga cgatggaata cggcccgttc gatttgccgg
      241 tggcgcgaac ggacggtgaa ggccaccgct ggcagtaccg ctacgataaa gacacgctgc
      301 aactgacaga agtcattaac ccgcagggcg aatcatacct ttatgttctg gataactgtg
      361 gccgggtgac ggaagagcat gactggggcg gcgtggtctg gcgttaccgc tatgacgccg
      421 atggcctgtg taccgccagg gttaacggcc tggaggaaac tatcctctac agccgggacg
      481 ccgcaggccg cctggcagaa atcatcacgc cggaaggcaa aacgcagtat gcgtatgaca
      541 aatccggcag gctgacgggt atcttcagcc cggacggtac atcacagcgc accggctatg
      601 atgaacgcgg gcgggtgaat gtcaccactc agggccgacg ggccattgaa taccactacc
      661 ccgatgaaca caccgtcatc cgctgtatcc tgccaccgga agatgaacgc gacagacacc
      721 ccggtgaatc cctgctgaaa accacgtacc gctacaacgc cgccggagaa ctgacggagg
      781 ttatcctgcc gggggatgag acgctgacgt tcagccgtga tgaggcagga cgtgaagtgc
      841 tccggcacag taaccggggt tttgcctgtg aacagggctg gaatgcagcc ggtcagcctg
      901 tcagtcagcg cgccggtttt ttcccggaag aagccacatg gggcgggctg gttccgtcac
      961 tggcgcggga gtaccgttac gacagcgcgg gtaatgtgtc aggcgtcacc agccgggaag
     1021 attacggacg ggaaacccgg cgggaatacc ggctggaccg gaacggccag gtcacggcgg
     1081 tgacagcctc aggcaccggg ctgggctatg gcgaaggcga cgagacttat ggctatgaca
     1141 gctgcggcta cctgaaggcg cagtctgcgg gcaggcaccg gataagtgaa gagactgacc
     1201 agtatgccgg aggccaccgg ctgaaacagg ccggaaacac gcagtatgac tatgacgccg
     1261 caggccggat ggtcagccgg acaaaacacc gtgacggcta ccgcccggaa acagagcggt
     1321 tccggtggga cagccgggac cagctgaccg ggtattgcag cgcacagggg gagctgtggg
     1381 aataccgcca cgacgccagc ggcagacgaa cagagaaacg ctgcgaccgg aagaaaatcc
     1441 gcttcacgta cctgtgggac ggcgacagta ttgcggaaat ccgggaatac cgcgatgatg
     1501 aactgtacag cgtacggcac ctggtgttta acggctttga gctgataagc cagcagttca
     1561 gccgggtacg gcagccgcat ccgtccgtgg ccccgcagtg ggtgacgcgg acgaatcatg
     1621 cggtgagcga cctgacgggc cgtccgctga tgctctttaa cagtgaaggt aaaaccgtct
     1681 ggcgaccggg cagaccagcc tgtgggggct ggcactcagc ctgcccgcag acaccggcta
     1741 cccggacccg cgcggggaac tggacccgga agccgacccc ggcctgctgt atgcggggca
     1801 gtggcgggat gcggaatcgg ggctgtgcta taaccggttc cggtattacg agccggagag
     1861 cgggatgtac ctagtgagtg atccactggg gttgcaggga ggggagcaga cttaccggta
     1921 tgtgccgaat cctttaagat ggatagatcc cttaggatta aataaaggag cttcattatc
     1981 taaaatgatg aatagctcca gtgatctcat ggggttgaga aggcagcccc agaacttctg
     2041 gcggctatat cgcggaaaag acatttaata atagcattac caggctcaga tgaattcaga
     2101 atgcttgaac attttggagc cgaagcttcg gtattagaca tgacgattat tgttcgttca
     2161 aatccgagta aagccgctat tcttgaagag tttttacatg gtacacaaga gaaactagga
     2221 atagctgaaa aattaggacg ttatggacta gggagtgcag aaacgcacgt taaggatttc
     2281 atgataagac acaaaaaaat gcttgggtta tctgatgagg atgtagcaat actcaaaata
     2341 ttgaaagaca ag
//
LOCUS       DAZGVQ010000310         2350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000310 DAZGVQ010000000
VERSION     DAZGVQ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.313"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2241
                     /locus_tag="ACHLAG_005220"
     CDS             <1..2241
                     /locus_tag="ACHLAG_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0658275.1"
                     /translation="HSDFSYDALDRLVQQGGFDGRTQRYHYDLTGKLTQSEDEGLVTL
                     WYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGHRVAVHYGYDDKGRLTGER
                     QTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGMKLGDTPL
                     LEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQSQHLNSLVYDRDYGWNDNG
                     DLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYATDPAGNRLPDPELHPDSTL
                     TAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDDERTHHYHYDSQHRLVFYT
                     RIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEMTWYGWDGDRLTTV
                     QTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHGVVFPAEL
                     VRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHFYHCDHRGL
                     PLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLYYNRNRYYD
                     PLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDDSYGTSSID
                     ITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAAITYSETKS
                     LPTSGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASVMTLACRTW
                     QECWVN"
BASE COUNT          572 a          590 c          740 g          448 t
ORIGIN      
        1 cacagcgact tcagctacga tgcgctggac cggttggtac agcagggcgg ctttgacggg
       61 cggacgcaac gttatcatta tgacctgacc ggaaaactca cgcagagtga agatgagggg
      121 cttgtcaccc tctggtacta cgatgaatcg gaccgtatca ctcaccgcac ggtgaacggc
      181 gaaccggcag agcagtggca gtatgatgac cacggctggc tgacagacat cagccacctg
      241 agcgaaggcc accgtgtcgc tgtccactac ggctacgacg ataagggccg cctgaccggc
      301 gaacggcaga cggtggagaa cccggagacg ggggaactgc tgtggcagca tgagacaaca
      361 cacgcctaca acgaacaggg gctggcaaac cgcgtcacgc cggacagcct gccgccggtg
      421 gagtggctga cctacggcag cggttatctg gcgggcatga agcttggcga cacgccgctg
      481 ctggagtaca cgcgcgaccg catgcaccgg gagacggtgc gcagctttgg cagcatggca
      541 ggcagtaatg ccgcatataa actgaccagc acatacaccc ccgcaggcca gttacagagc
      601 cagcacctga acagcctggt gtatgaccgt gactacgggt ggaatgacaa cggcgacctg
      661 gtgcgcatca gcggcccgcg acagacgcgg gaatacggct acagcgccac gggcaggctg
      721 gagagcgtgc gtaccctcgc accggacctg gacatccgca tcccgtatgc caccgacccg
      781 gcgggaaacc gcctgccgga cccggagctg cacccggaca gtaccctcac ggcgtggccg
      841 gataaccgca tcgcagagga tgcgcactat gtttaccact atgatgaata cggcagactg
      901 acggagaaga cggaccttat cccggcgggt gttatccgga cggacgacga gcggacccac
      961 cactaccact acgacagcca gcaccgtctg gtgttctaca cgcggataca gcatggcgag
     1021 ccactggtcg agagccgcta cctctacgac ccgctgggac ggcgaatggc gaaacgggta
     1081 tggcggcggg agcgtgacct gacggggtgg atgtcgctgt cgcgtaaacc ggagatgacg
     1141 tggtacgggt gggacggcga ccggctgacg acggtacaga ccgacaccac gcgtatccag
     1201 acggtatatc agccggggag cttcgcgccg ctcatccgca tcgaaacaga caacggcgag
     1261 cgggagaaag cgcagcgccg cagcctggcg gagaagctgc agcaggaagg gagcgaggac
     1321 gggcacggcg tggtatttcc ggctgaactg gtgcggctgc tggacaggct ggaggaagaa
     1381 atccgggcag accgcgtgag cagtgaaagc cgggcgtggc ttgcgcagtg cgggctgacg
     1441 gtggagcaac tggccagaca ggtggagccg gaatacacac cggcgcgaaa agttcatttt
     1501 taccactgcg accaccgggg cctgccgctg gcgctcatca gcgaagacgg caatacggcg
     1561 tggcgcgggg agtatgatga atggggcaac cagcttaatg aagagaaccc gtattacctg
     1621 caccagccat accgtctgcc ggggcagcag catgatgagg aatcagggct gtactataac
     1681 cggaaccggt actatgaccc gctacagggg aggtatatta cacaagaccc cattgggctg
     1741 gcggggggat ggaacctgta taattaccca ctgaatccga taataaggat ggatcctttg
     1801 ggtttgtata atttatatca attattatat gatgtttggc atgatgattc atatggaaca
     1861 tcatcaattg atattactgg cagtggagat ctaatatcat taggtggtca tgcaggactt
     1921 ggcgttgcgt ttgctaaaaa gaaaggtgaa atgttatctg atatttgtat ttatgctaca
     1981 gcatgcggac atgcaggaat tggtggtggg ataaatgcgg ctatcacata ttctgagacc
     2041 aagtctttac ctacatcggg agtcagcaat tcagtaggtg taacggttgg cggcggagtt
     2101 ggggggcatt ttgcgtatac ttatgtagtg gatgttgata atccagaatc atcgacagaa
     2161 tctgttggta tcggtgcagg tgttgacgct tcagttatga ctctggcttg tagaacgtgg
     2221 caagaatgct gggtcaatta acaggaggaa atatgaaaaa atatatattt ttagttttat
     2281 ttttattgtg tggttgttct ccagtggaca tagagccagc tagacaagaa gttaatatct
     2341 ttcgtggttt
//
LOCUS       DAZGVQ010000311         2337 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000311 DAZGVQ010000000
VERSION     DAZGVQ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.314"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(139..552)
                     /locus_tag="ACHLAG_005221"
     CDS             complement(139..552)
                     /locus_tag="ACHLAG_005221"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658276.1"
                     /translation="MGAKFLDQKTGVILEEPEFVKLYIRDLCRVKGLSATQYKIFTFM
                     LANMNTDNVVSFGPRTKEKFLSEHSIKPQTFNNNVSQLIESCLISRIARNEFLVNKKY
                     AVKVDWAKVQKIVWTTTYSNEGIDSTVTIAPKKKD"
     gene            1961..>2337
                     /gene="mobA"
                     /locus_tag="ACHLAG_005222"
     CDS             1961..>2337
                     /gene="mobA"
                     /locus_tag="ACHLAG_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFP0658277.1"
                     /translation="MTKNKRSGSEVRQRTKVITLRVNNRIANEIRRRAKSEGLTISEY
                     IRTTSLNNEIKQRVPSRYLDELIQLGRMQKKLFDKGKRPKDKEYLDVMHKIISLCAEM
                     KEVTQRISDIYKKMDLIKEEIKA"
BASE COUNT          697 a          486 c          487 g          667 t
ORIGIN      
        1 aaaaaaaata cataaaaata aatacccagg ttccaactta tttaattgag cagaacatag
       61 gtcttcataa aaatttaaag acctataata gaattagcga cattttgttt tatcgaaatc
      121 ttttacaatc ataagaaatt aatccttttt tttcggggct atcgtcactg ttgaatcaat
      181 cccttcatta ctgtaagttg ttgtccatac tatcttttgt actttcgccc aatcaacttt
      241 aactgcgtat tttttattaa ccaaaaattc atttctggct attctagaaa ttaaacatga
      301 ctcaatcagt tgagacacat tattattaaa tgtttgtggt ttaattgaat gttctgataa
      361 aaacttttct ttggttcttg gtccaaatga aacaacatta tcagtattca tattagcaag
      421 catgaacgta aaaattttgt attgcgttgc tgatagccct ttaacacggc ataaatcacg
      481 gatatacagc ttcacgaact ccggctcttc caaaattacg ccagtttttt gatctaaaaa
      541 tttcgctccc attctcacct cgcaggtaat ttagttaccg ttttatcggt atcttatgta
      601 tgttccacaa ccgagtaaat actttttttc cggtatttga ttagcaacaa aaacagtatt
      661 ccggtatttg aaataccgct gagcggtatt tcaacctccg ctcagcggta aaaactttcc
      721 cgctgagcgg tatatctatt cccgctgagc ggtatttagt ttgcctctag gccttgtcac
      781 acctgatttt cctatctgtc tgtgaatcta tcttagatct cgtgcgtaag tgcactatgc
      841 caatactggt tcccttccgc ttcctcgccc actcgcgagc ctagcggctc ggtcgctggg
      901 gctgcggcga gcggtgccac ctctcgcata tcgttgacga tcccgatttg atctcaggcg
      961 atcgagtcgc tggccaccgg ttaaacgcgc tcaaagcctc ccagacgcac aaaacggggg
     1021 ttaaatgccc gtcagggcat ggaaggcgcg aaaaaggctt acagaggttt tcagaaggtt
     1081 aggcgcgccg atacccctaa ccaggggctt tcaggtcgct aggaaccgcg ccagccccct
     1141 gcaaggccca tttgcacgga acagacagcc actcggttgg taggtggtcg tagaccgtcg
     1201 gtgttacagc gatgggggtc tggggttctc cccagacggt ttccggttgc gctcaaggcg
     1261 ctgccggaac cgtggcggct tcgatggtga agggtgagcg cgtttccaag aggttttcca
     1321 ggcaagctat gactgaactg aacagattga acgaggagga aacgcgctca gacgctttgt
     1381 agcgcgcttt tctgtgtcag gcaagtttgt tgtgtctttt gcctttcagg ttgttagcag
     1441 ctggcggagc actgcacggg gattaatgct cccattgcta ggatgaaata aagattagga
     1501 gcgaagttaa gttatatttt taatatataa acgcgatatc aataactcac atagttatat
     1561 tttatgcata tgctaccatc ttgctctttt gcctcctgct ctctggacga atcccgcatt
     1621 accaacaggc aatggattat ataaccaaga agatctaatc tttattcctc taataatcca
     1681 caaacgacag acaacaaaga catcaattaa ttcggtgtta tttattaaat aacaagcaat
     1741 aatttttaaa atctgtgtaa ataatattga atgaagcatc atttttttgt atcagaaata
     1801 ccaggttacg acgcggtatt ttattttcgt cgaaaagttg cagcggaaaa tgggacagga
     1861 tgtgtattcg tcataccgcg aatacattcg cttgcatgag aatacctcct gtcagggcaa
     1921 gccccgacac cccaaacgtg caaaatggag aatcattgcg atgactaaaa ataagcgtag
     1981 cggttcagaa gtacggcaga ggactaaggt tatcaccctt agagtaaata acagaatcgc
     2041 aaacgaaatc agacggcgag cgaagagcga agggctgact atttcagaat atataaggac
     2101 aacaagctta aataatgaaa taaaacagcg cgttccttcc agatatttag atgaattaat
     2161 ccaacttggt cggatgcaga agaaattatt tgataaaggg aagagaccga aggacaaaga
     2221 gtatctggat gtgatgcata aaataatttc actttgtgct gaaatgaaag aagtaacaca
     2281 gcgaatttct gatatatata aaaaaatgga tttaataaaa gaagaaataa aagcaat
//
LOCUS       DAZGVQ010000312         2333 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000312 DAZGVQ010000000
VERSION     DAZGVQ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.315"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            475..708
                     /gene="ykgL"
                     /locus_tag="ACHLAG_005223"
     CDS             475..708
                     /gene="ykgL"
                     /locus_tag="ACHLAG_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HFP0658278.1"
                     /translation="MKTQAAPQREETEWRVQSKRGLMPAYRGEAGQQVNIKIMEYSER
                     NVRQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(743..883)
                     /gene="ykgO"
                     /locus_tag="ACHLAG_005224"
     CDS             complement(743..883)
                     /gene="ykgO"
                     /locus_tag="ACHLAG_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFP0658279.1"
                     /translation="MKVLNSLRTAKERHQDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(883..1146)
                     /gene="ykgM"
                     /locus_tag="ACHLAG_005225"
     CDS             complement(883..1146)
                     /gene="ykgM"
                     /locus_tag="ACHLAG_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP0658280.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            complement(1509..1610)
                     /gene="ykgR"
                     /locus_tag="ACHLAG_005226"
     CDS             complement(1509..1610)
                     /gene="ykgR"
                     /locus_tag="ACHLAG_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFP0658281.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            complement(2084..2173)
                     /locus_tag="ACHLAG_005227"
                     /pseudo
     CDS             complement(2084..2173)
                     /locus_tag="ACHLAG_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          679 a          437 c          524 g          693 t
ORIGIN      
        1 tatattggtc aggaactttg ccctgaagtg ttaggaatag tcttaatgcg ctgtgtgatg
       61 gactagtaaa aaaacaagtt ttaagggtaa gggatagaat ataaaactat aatgagctgt
      121 cattgaagat gatttgccaa tcagtaagaa attttaatgg attaacatgc cgcacaaaat
      181 ggttataggt gttcattaca ataagtgatg gaaaattgaa cgggcgtatg tacgtcatct
      241 gttcggtaag acccccaaga gccagattaa gtgaagttaa acgaaggctg ccagctgtca
      301 caggaacgat ggcctctttg tcagggcact gagtgcagtt atctgtgcgc gttactctaa
      361 ctggcactcg aacgtcggcg atgggcttac cggtggatca aactgttcct gtggcgtaaa
      421 cgtttttatg ttagtaccaa cagatttacc acatttatca tcttaacagt ttgagtgaaa
      481 acgcaggctg cgccgcaaag ggaggagact gagtggcgtg ttcagtctaa aagagggttg
      541 atgccagcat acaggggcga agctggtcaa caagtcaaca ttaagataat ggaatattcg
      601 gagagaaacg tcaggcagtt ggcctcaaat gaacaggaag aatatatccc caggaaaatt
      661 aatgttggtg ttataaacac accaacatta atccgatctg attattaatc agacaaatat
      721 cgttaattcg tcgaattttg aatcaacgtt ttttcttacg accctgaacg gccttaaaac
      781 gtggattaga tttacaaatc acatacaacc gtccttttcg cttcacaatc tgacagtctt
      841 gatggcgttc ttttgcggta cgcagagagt taagaacttt cattacgccc cctttttcgt
      901 gctaacaaaa cgaccaaaac gttgggtgaa ccgtgcaaca tttccttctg atgccactgt
      961 tctcagcttc cctgtataga acgggtgcga tttcgaagag acatcaattg ttacgtacgg
     1021 atacgttacg ccatccagct caatctcacg gtctgttttg atagtcgagc cgattttaaa
     1081 gtactcatca acactggtgt cgtggaacac cacagtacga tactcaggat ggatattggg
     1141 cttcatcatt tttacctgtt atgttataac ataatcataa agatacatgc tcatccggat
     1201 catttcaaca cccaatccct gtgactcttt gtggatgtgg aattttcgca acggggaact
     1261 ctggcattgt tagcagccat tggtgacgct cccataaacg cctggatgag tgattgatga
     1321 tggttttgct gagagtatgg atttttcgac aatggaggtt atctccactt tactggccga
     1381 tgatttatcg cagtatgatt gattacctga cagcgtgtat tcctctccca cggatataat
     1441 aattacttac aaaaagggag aggatgcata ttttaaatat cactgaagtg aacagtttat
     1501 tgccgttatt aatagaaatg gagaaataaa taggcgtatt ctacaattgc gacaaaaaca
     1561 acgatattaa tcagtttatg actgatttgt tgtactttat tctctttcat tggtacttcc
     1621 tcgctttaaa aaagagcgca cttcgtaagt gcccttatat aaataacgag tttggtcaac
     1681 caattttttg acatgtatca caaatttgaa tagatgtatt acatcggcta tcttttattg
     1741 caccaacgtc attgatatat gtcgcctgaa gtcagttccg ggaatgagtc tgatctcaag
     1801 actggcccag tccgggcgtt gattggcgct gaggagcata tcgcatctca tcataatgtc
     1861 gtatctcctg gggtgttata caagatatcg ttgttggtga cctgggagag gaattgagtt
     1921 ctattaaacc gtcaactatg ccggatacat actggattac actgcaggca cgccttacga
     1981 gagaacgtgc cgcagtgacg ggttaattat ctgaaagaat ttgtgaggct gtatcggtta
     2041 ctcattgatt tgatagtttt actctcggga gagtaataga tatttaatct attaacggaa
     2101 accagccagt tcctttcgat gcctgaattt gattccatag tttatccgga atggttaatt
     2161 catgaataag ttgtgttctc gttcatgaga agcatgacgt aaagggaaaa gctcgattag
     2221 acggcaggat ttgtcagggg ttatgaacga aattcataaa tctgtttgag tgttgcgatg
     2281 ggtagtgcaa gttcgaaatc tccgcaattt acagtccgat gaaggaaaat gaa
//
LOCUS       DAZGVQ010000313         2322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000313 DAZGVQ010000000
VERSION     DAZGVQ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.316"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..535
                     /locus_tag="ACHLAG_005228"
     CDS             <1..535
                     /locus_tag="ACHLAG_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0658282.1"
                     /translation="YEAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLV
                     GATVVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLTAMTASFVLATPTE
                     TDGALFPGRIMLANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKYMRGCEMR
                     GMAVNFGGFLSINKLSQ"
     gene            546..1289
                     /locus_tag="ACHLAG_005229"
     CDS             546..1289
                     /locus_tag="ACHLAG_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0658283.1"
                     /translation="MTQTESAILAHARRCAPEESCGFVISTPEGNLYQPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVSRGEIHR
                     FRCVPHLTGRRFEHGVTDCYTLFLDAYHLAGIEMPDFHREDDWWRNGQNLYLDNLAEN
                     GFYRVSPSCAQAGDILLCCFGASVPNHAAIYCGNGELLHHIPEQLSKRERYSEKWQRR
                     THSVWRHRHWSASAFTGIYNDLVAASACM"
     gene            1286..1867
                     /locus_tag="ACHLAG_005230"
     CDS             1286..1867
                     /locus_tag="ACHLAG_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0658284.1"
                     /translation="MNTAAEAIRALSLQVPGFRCQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEPLSGRAVIHIVPRLAGAGGNGVFQVVLGAAAIVGSFFTAGAMMALWGAALSAGGL
                     TATTMLFSLGASMILGGVAQMLAPKAKTPEYKSTDNGRQNTYFSSLDNMIAQGNPMPV
                     PYGEMLVGSRRISQDISTRDEGSDGKVVVIGRG"
BASE COUNT          503 a          558 c          753 g          508 t
ORIGIN      
        1 gtatgaggcg tacccgattg agggcagcgg ctttgagatg aacggaaagg gcagcagtgc
       61 ccgcccgtcg ctgacggtgt ccaatctgtt cggccttgtc accgggatgg cggaggattt
      121 gcagagcctg gtgggtgcca cggtggtccg ccgccgggtg tatgcccgtt ttctggatgc
      181 ggtgaatttt gtggcgggga atccggaggc cgacccggag caggagctga cggaccggtg
      241 ggtggtggag cagatgtcat cgctgacagc catgacggcc tcgtttgtgc tggcgacacc
      301 gacggagacg gacggggcgc tgtttcccgg tcgcatcatg ctggcgaaca cctgtatgtg
      361 ggattaccgg ggcgatgaat gcgggtataa cggtccggca gtggcggatg agttcgacaa
      421 ccccaccacg gatatccgga aggacagatg cagtaaatac atgcgcgggt gtgagatgcg
      481 cggcatggcg gttaattttg gcggtttcct ttccattaat aaactttcgc agtaaatcct
      541 gttttatgac acagactgaa tcagcgattc tggcgcatgc ccggcggtgt gcgcctgagg
      601 agtcgtgcgg cttcgtgata agcacgccgg agggcaacct gtaccagccg tgcgtgaata
      661 tctctgcaga gcctgaggca tattttcgta ttgcaccgga agactggctg cgggcagaga
      721 tgcaggggga gattgtggcg ctggtccaca gtcatcccgg tggtctgccc tggctgagcg
      781 aggctgaccg gcggctgcag ataaaaagcg cactgccctg gtggctggtc agccgggggg
      841 aaattcaccg gttccgctgt gtgccgcacc tgaccggacg gcgctttgaa cacggtgtga
      901 cggactgtta caccctgttc ctggatgcat accatctggc ggggattgag atgccggatt
      961 ttcaccgcga ggatgactgg tggcgcaacg gtcagaacct gtacctggac aacctggcgg
     1021 aaaacggctt ttaccgtgtg tctccgtcct gtgcacaggc aggcgatatc ctgctgtgct
     1081 gctttggcgc atcggtgccg aatcatgccg ccatttactg tggcaacggt gagctgcttc
     1141 accatatacc tgaacaactg agtaaacggg agaggtattc agagaaatgg caacgacgaa
     1201 cgcattctgt ctggcgtcac cgccactggt ccgcatctgc cttcacgggg atttacaacg
     1261 atttggtcgc cgcatcagcc tgtatgtgaa cacggcagcg gaggctatcc gtgccctgtc
     1321 gctgcaggtg ccgggattcc gctgtcagat gaacgaaggc tggtaccaga tacgtattgc
     1381 cggtgaggat accgcgccgg aggcggtgta tgcccgtctt catgaaccgc tgagcgggag
     1441 ggccgtgatt catattgtac cgcggctggc aggggccggg ggaaatggtg tttttcaggt
     1501 ggtgctgggg gcagcagcca tcgtgggctc tttcttcacc gccggtgcaa tgatggcgtt
     1561 gtggggcgca gccctgagtg ccggagggct gactgccacc acgatgctgt tctcactggg
     1621 tgccagcatg atactgggtg gtgtggccca gatgctggcc ccgaaggcaa agacgccgga
     1681 gtacaaaagc acggataacg gcagacagaa cacgtatttt tcgtcactgg acaacatgat
     1741 tgcccagggg aacccgatgc cggtgcctta cggtgaaatg ctggttggct cacgacggat
     1801 atcccaggac atcagcaccc gtgatgaggg cagtgacggg aaagtggtgg ttatcgggcg
     1861 aggatgaaaa taaaaaaatc ccgcagtgct cggcgcctgc gggaagagaa cgatgttgac
     1921 taacctgttg gcgttttatt tttatttacc cgcaaaaaat gtaacgcagc gtcattattg
     1981 tggctacagg taatttgcag aaatgtgaat aaattcagac tttttatccg gtagcggggg
     2041 agcggaagcc aggcgggtat ctgtggaaga agacagataa catcgtgccg ggtttaaggg
     2101 ataaaaaaat cccgcagaat cagcggagct gcgggagaga acgatgaaga ttaacgttat
     2161 ggagttattt ttcaggcatc aaaaatgtaa cgcagcgtca ttattgcggc tacaggcaat
     2221 tgccggaaat gtgaagaatt tcagaaattt tattccgtca tgacacaggc accctccggg
     2281 gtgcctgttg tttttgggca taaacagatt cagacatcag ac
//
LOCUS       DAZGVQ010000314         2275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000314 DAZGVQ010000000
VERSION     DAZGVQ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.317"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            819..1829
                     /locus_tag="ACHLAG_005231"
     CDS             819..1829
                     /locus_tag="ACHLAG_005231"
                     /inference="COORDINATES: protein motif:HMM:NF013603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFP0658285.1"
                     /translation="MKNPDSPILSLRDYSTQDSKWDSDRARADQVAKIYASDQAFFRR
                     GERMFDCSQRLQFAPQSSRLTGEMRLALRHGEFCHVPFCPVCSRRRSLRWMRRLWEAL
                     PKLLAEHPTASWLFLTLTVKNPPVGELRETLRQMNAAWERLIKRKAFAPVLGWLRSTE
                     ITYGNVRGCCHPHFHVVLWVPSSWFTRDYVKHERWVEIWSECLRVDYAAGAHIKRVRP
                     KRGKVPEGASFAEVQRAALEAGVIEMLKYTVKSSEIVRDPAWFLELARQTYGLRMIAT
                     GGRFREILQVEKPETDEDLIGADMPAKPDEFEEMAFWLAFDWRRDEKRYKRNPKADRR
                     KE"
BASE COUNT          488 a          528 c          707 g          552 t
ORIGIN      
        1 aaaaaaatca tgaaatgcgc acttatacaa tctccactcc gttccgattg tgtgcggtct
       61 gcgacgctca aagaaacggc aaaatgattt gttgtccggt cgtcgaaaat gccgagcctc
      121 atctgcccat ctgccagcgt tcaggcgaaa aaatcgggac aggatgtgtg tttgtcggac
      181 cgcgcacgcg ccatcaaaca aacaccatct ggtcagggct gcgccccgac accccgccaa
      241 cgtctcacac agccagattg aaatctgtca gcgttcgccg ttccctgact cgtcaaacag
      301 aaaatcgcgg ggtcccgcgc ctccggcgtg ggttccgatt ttgtgattga cgacatggcg
      361 gataaaaaca ctgggagtgg tggttggtgt cacgcagtga ggccaaccac ctgccggacg
      421 gttttttgtt gttcacagat tcgggtgaaa aggttgtgga tggggtgggt gtaacaggcg
      481 cgaaaaacgg ggttttgcgg tgctgaaaat ctgcgcaaat attgagcgat tttggggtgc
      541 gccttgtatt tatactatag gcaacaactc gcggatgcat ttttgccttt cagcccagta
      601 atggcgcggg tttgaggggt tttttagttg tagatggttg ttaaattgtt tttacaactt
      661 tttttggcta cagtgtgagc aacaggttgg tgaggttccc cggcagtgtg ctaccactcc
      721 gggggcgacc acctgaaagg taaaattcgg taagccaagc acaggtggta agaacatgct
      781 aacacaagat ttctgcccct gcctactgag ggcaggtcat gaaaaatcca gatagtccga
      841 tcctctcact gcgtgactac agcacgcagg attcaaaatg ggacagcgat cgcgcgaggg
      901 ctgaccaggt ggcaaagatt tacgcgtcag accaggcatt tttccgtcgc ggcgagagga
      961 tgtttgattg cagtcagcgt ttacagttcg ctccacagag ttcccgtctt acaggcgaga
     1021 tgcgacttgc actgcgtcat ggtgaattct gtcatgtccc gttttgtccg gtgtgtagcc
     1081 gtcgccgctc gttacgctgg atgcgtcgtc tgtgggaggc gctaccgaaa ctactggctg
     1141 agcaccctac tgcgagttgg ttattcctga ccctgactgt aaaaaatcct cccgttggtg
     1201 agttgcgcga gacactccgg cagatgaatg cggcttggga gcgtttaatc aagcgcaaag
     1261 cgtttgcgcc tgttctggga tggctgcgga gtacggaaat tacctacggg aatgtgcgcg
     1321 gatgttgtca cccgcacttc catgtggtgc tctgggttcc ttcgtcatgg ttcacgcggg
     1381 attacgtgaa acatgagcgc tgggtggaaa tctggagtga atgcctgcgg gtggattacg
     1441 cagctggcgc gcacatcaag cgcgtgcggc caaaacgggg gaaagtgccg gaaggggcaa
     1501 gttttgctga ggtgcagcgg gcagcgctgg aagcgggcgt gatcgagatg ctgaaataca
     1561 cggtgaaatc atcggaaatc gtgcgcgatc cggcgtggtt tctggagctg gcgaggcaga
     1621 cctacgggct gcggatgatt gccacgggag gacggtttcg ggagatcctc caggtcgaga
     1681 agccggaaac ggacgaggat ttgattggtg ctgatatgcc agcgaagccg gacgagttcg
     1741 aggagatggc gttctggctg gcgtttgact ggagacgtga cgagaaacgc tacaagcgga
     1801 atccaaaagc agacaggcgg aaggagtgag cgtgtttcag aggcacattg tgggaatagt
     1861 gcggtagtgg aggtgaaacg cgctcagaag cgttctaacg cgattttcag aaacgggcaa
     1921 gtatttgccc gttttttcgt tttaaggcgc tcagaagcgt tttgagcgcg tttctggcgt
     1981 tgtcgactgt aactctctct ggacttccgg tttcctgggg tgccagaaac gcgcgtttgt
     2041 cgttttagcg tgtcgctggc acggtgtcta gggtgtcggg gcgcagccct gaccagatgg
     2101 tgtttgtttg atggcgcgtg cgcggtccga caaacacaca tcctgtcccg tttttgcttc
     2161 cggttttttg gattttctcc ggcgtggtaa cggaacattt cggaaatgtg acggcaaatc
     2221 attttgcggt ttttgcgaac gagttggacc tcacgagcgc cagattgctg cgcgg
//
LOCUS       DAZGVQ010000315         2235 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000315 DAZGVQ010000000
VERSION     DAZGVQ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.318"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1601
                     /locus_tag="ACHLAG_005232"
     CDS             <1..1601
                     /locus_tag="ACHLAG_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFP0658286.1"
                     /translation="EKGDPGGLAGPQGPKGERGEAGPQGPMGARGERGETGPRGEPGP
                     AGPRGERGETGPQGPRGEPGPAGSAANVADATTAQKGIVQLSSATDSDDETKAATPKA
                     VKAAMDKADGCLEKAKNGDDIPDKVKFLNTVGAARVYGRDIHTGAGEWTTSEFVAWLK
                     EKGAFDQPYWMMKASLHAEFNKVITDVGPGKLNLGGCAIEVMGTYNAAIVRVTIGEYG
                     GDGFLNGTVCTCTVYGDTQRFHWRVDYSTKNKPDTVSQRDASTTQKGVVQLSSDTDSN
                     DETKAATPKAVKAAMDVANEAKTKAEEAAAGGGVPGPKGEKGDTGPAGPAGPQGPKGD
                     TGAAGPAGAQGPKGDKGDPGVAGPAGPAGAPGPKGDKGDPGVAGPAGPEGPQGPKGDT
                     GAPGQGTELLTTANTWTQAQTFNGGINGNLTVNGNGSFNDVQIRSDKRNKRNLVKLDN
                     ALDRLEALTGYLYEIQYSADGWQTSVGLIAQDAQKALPELVTEDADVISGEKRLRLNY
                     NGIIALLVEGFKTLRHEIKELREK"
     gene            1688..2200
                     /locus_tag="ACHLAG_005233"
     CDS             1688..2200
                     /locus_tag="ACHLAG_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658287.1"
                     /translation="MGITSGWVGSSAKSETGEQWMGAAGTKLGLDKPFMMSQMVGRAM
                     GCKIETAYYKWNSSDQVENWGAVGADWPLEEKSKGTITNAENCGSGRLVGAVVTLSHF
                     LTNSTPTAAVYLSGGKAGNITVNVGGATQTMIYQGVVSGFQYYWSGSVSSAFVEAMKK
                     TGVTQDLKIS"
BASE COUNT          642 a          450 c          701 g          442 t
ORIGIN      
        1 gcgagaaagg tgatccggga ggtcttgcag gcccgcaagg tcctaaaggt gaacgaggag
       61 aagccggacc acagggtccg atgggagcac gaggtgagcg tggggagact ggcccccgag
      121 gtgaacctgg tcctgcaggt ccgagaggcg aacgaggaga gaccggacct cagggacctc
      181 gtggagagcc aggtccggca ggcagcgctg caaatgtggc tgatgcgacg acggcacaga
      241 agggaattgt gcagttaagc agcgcaacgg acagtgatga tgaaacgaag gcagccaccc
      301 cgaaagcggt gaaagcggca atggacaaag cggacggatg cctggagaaa gcgaaaaacg
      361 gtgacgatat cccggataag gtgaagtttc tgaacaccgt gggagcagcc agagtatacg
      421 ggcgggacat tcatacgggg gccggtgaat ggaccacgag tgagtttgtg gcctggctga
      481 aagaaaaggg ggcatttgac cagccttact ggatgatgaa ggcatcactg catgcggaat
      541 ttaataaggt catcacggat gtcggaccgg gaaaactcaa tctgggaggc tgcgccattg
      601 aggtgatggg gacgtataac gcagccatag tccgggtcac catcggcgaa tacggtggcg
      661 atggttttct gaacggcacg gtctgtacct gtacagttta cggagacaca caacgttttc
      721 actggcgggt ggattacagc acaaaaaaca aaccggatac ggtcagccag cgggatgcca
      781 gcacgacgca gaaaggtgtg gtgcagttaa gcagtgatac tgacagtaat gacgaaacaa
      841 aggcagccac gccgaaggcc gtgaaggcgg caatggatgt ggcaaatgaa gcgaaaacaa
      901 aggcagaaga ggctgcagca ggaggtggtg ttcccggtcc gaaaggagag aaaggggaca
      961 cggggccagc aggtcctgca ggaccgcagg gacctaaagg ggatacggga gctgcaggcc
     1021 cggcaggcgc acagggacca aaaggtgaca aaggcgatcc gggggtggct ggaccagcag
     1081 gtccggcagg tgcgccgggg ccgaaaggcg ataaaggtga tccgggagta gcaggtccag
     1141 caggtccgga agggccgcag ggaccgaagg gagacactgg agcccccggg caaggaacag
     1201 aactgcttac tactgccaat acatggactc aggcacaaac ttttaatggt ggtattaatg
     1261 gaaatttgac ggtaaacgga aacggatcat ttaacgatgt tcagatccgc tcggataaac
     1321 gcaacaagcg aaatctggta aaactggata atgcgttaga tcgtctggag gcacttactg
     1381 gttatcttta cgagatacag tactctgccg acggttggca aacgtcggtt ggtttaattg
     1441 ctcaggatgc acaaaaagcc ttgcctgaac tggtaactga agacgcagac gttatatctg
     1501 gtgaaaaacg tctgcgtctt aactacaacg gcataattgc attgttagtc gaaggcttta
     1561 aaacacttcg tcatgagatt aaagaactcc gggagaagta aacgacagct gttgtagttt
     1621 ctggtttcta ctgaatttaa attgtgggga tgatactcac ctcacgaatt tcagaaggat
     1681 atatgaaatg gggataacat cgggatgggt aggttcttcg gctaagagcg aaacaggtga
     1741 gcaatggatg ggggctgctg gcactaaact aggattggat aaacctttta tgatgagtca
     1801 aatggtgggg cgagctatgg gttgtaaaat agaaaccgct tactataaat ggaactcttc
     1861 ggatcaagtt gaaaactggg gggcggttgg agctgattgg ccattagaag aaaaaagcaa
     1921 agggacaatt acaaacgctg aaaactgtgg ttctgggaga ctggtggggg ctgtcgttac
     1981 actttctcac tttttgacga actctacacc gacagctgct gtttatttat ccggtggtaa
     2041 agcaggtaac atcaccgtaa acgtaggtgg tgctacacaa accatgattt atcagggcgt
     2101 tgttagtggg ttccagtatt actggtcagg ttctgttagt tccgctttcg tggaggcaat
     2161 gaaaaagacg ggagtaaccc aggatctaaa aattagttaa atggtaaatg aataatttta
     2221 aaaacttcac gttct
//
LOCUS       DAZGVQ010000316         2206 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000316 DAZGVQ010000000
VERSION     DAZGVQ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.319"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..128)
                     /locus_tag="ACHLAG_005234"
     CDS             complement(<1..128)
                     /locus_tag="ACHLAG_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mov34/MPN/PAD-1 family protein"
                     /protein_id="HFP0658288.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGNQYQPCVNISAEP"
     gene            complement(139..837)
                     /locus_tag="ACHLAG_005235"
     CDS             complement(139..837)
                     /locus_tag="ACHLAG_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP0658289.1"
                     /translation="MQDIHEASLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSALTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            complement(837..1166)
                     /locus_tag="ACHLAG_005236"
     CDS             complement(837..1166)
                     /locus_tag="ACHLAG_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0658290.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVMLSVSREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(1163..>2206)
                     /locus_tag="ACHLAG_005237"
     CDS             complement(1163..>2206)
                     /locus_tag="ACHLAG_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0658291.1"
                     /translation="NQYVVLKEAATKRQLSEQEKSLLAHEKETLEYKRQLAELGDKVE
                     HQKRLNELAQQAARFEQQQSAKQAAISAKARGLTDRQAQRESEEQRLRDVYGDNPAAL
                     AKATSALKNTWSAEELLRGSWMAGMKSGWGEWAESATDSFSQVKSAATQTFDGIAQNM
                     AAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAFGGGASASTGTAI
                     QAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGG
                     YVGGAGSPAQMRRAEGINFNQNNHVVIQNDGINGQAGPQLMKAVYDMARKGAQDELRL
                     QLRDGGMLSGSGR"
BASE COUNT          429 a          793 c          522 g          462 t
ORIGIN      
        1 ggctctgcag agatattcac gcacggctgg tactggttgc cctccggcgt gcttatcacg
       61 aagccgcacg actccgcagg cgcacaccgc cgggcatgcg ccagaatcgc tgattcagtc
      121 tgtgtcataa accgggattt actgcgaaag tttattaatg gaaaggaaac cgccaaaatt
      181 gccgacattc ctgcgcagtt cacacccgcg catgcacttg ctgcatctgt ccttacggat
      241 atcggtggtg ggtttatcga actcatccgc caccgccccg cccgtgtaac cacactcatc
      301 agagcggtag gtccacatac aggtgttcgc cagcatgatg cggccgggaa acaaagcacc
      361 gtccgtctcg gtcggtgtgg ccagcacaaa cgaggccgtc atggccgtca gcgctgacat
      421 ctgctccacc acccaccggt ccgtcagctc ctgctccggg tctgcctcag gattgcctgc
      481 cacaaagttc accgcatcca gaaaacgcgc atacacccgg cggcggacca ccgtggcacc
      541 caccaggctc tgcaaatcct ccgccatccc ggtgacaagg ccaaacagat tggacaccgt
      601 cagcgacggg cgggcactgc tgccctttcc gttcatctca aagccgctgc cctcaatcgg
      661 gtacgcctca tacttccgcc cctgccaggt caccggctcc cctttttcat tcagctcatt
      721 gcagaaaaaa taccgctcac cgccctgcac cgtcaggtcg atttcccaga gcaccacccg
      781 cggtgactgc tctgacttaa ccgactcgtt caggcttgct tcgtgaatat cctgcatcag
      841 ttcaccacct gcttaaactc cgcgctgaac tcaacgcgca acatcccgac ccgcgcagac
      901 cacccggcac aggtcacctt tatctgccgg tatgcatagg gtggcttcca caaaaatgcc
      961 ttccagcccc cgtgctctgc caggaacgct tccagatgcc gggcctcctc ccggctcacg
     1021 gaaagcatca cccggtatgt tttcaggtca gcattcagcc ctgccgccat acgctgtgag
     1081 tacccgtcac caaaacgcac ttcacgcacc gatggctgcg agttcacctc catatccggc
     1141 ttcactttcc agcgaaaggt tttcatcgcc cgctccctga taacataccg ccatcacgca
     1201 actgcagccg gagctcatcc tgcgccccct tgcgggccat gtcatacacc gccttcatca
     1261 gctgcggccc cgcctgtccg ttgataccgt cgttctgaat caccacgtga ttgttctgat
     1321 taaaattaat accttccgcc cgccgcatct gcgccggact tccggcacca cccacataac
     1381 ccccttccgc atagccgcgc atcagacggt aaagattccc cacgcctatc cggctggttg
     1441 cctccttcgt gaagacaaac tccccgcggt gaactatccc cgcaggctca tatttgccgc
     1501 ccgtgcccgt aaatccgccg gtcgcaaaat gaaagttcgc cgccgcagcc tgaatggccg
     1561 tccccgtgga ggcagacgcc ccaccaccga aagcaccacc aatggcgctg ccgatacgcc
     1621 cgacaatgcc caccatggcc tgtttaagca ggatttctgt catcatggac agcaccgaac
     1681 gggtgaatcc ccgccagtct gcctctgcac cggtcagcat cgccgccata ttctgtgcaa
     1741 taccgtcaaa ggtctgcgtg gccgcgcttt taacctgcga aaaactgtcc gtcgcacttt
     1801 ccgcccactc gccccagccg gacttcatcc cggccatcca gcttccacga agcagctcct
     1861 ccgcagacca ggtgttcttc agtgccgatg tggccttcgc cagcgcagcc ggattatcac
     1921 cgtacacgtc acgaaggcgc tgctcttccg actcccgctg cgcctgacgg tcggtgaggc
     1981 cgcgggcttt tgcgctgatg gcggcctgct tcgcgctttg ctgctgttca aaccgcgccg
     2041 cctgctgtgc cagctcattc agccgcttct ggtgttcaac cttgtcgccc agctcagcca
     2101 gctggcgttt gtactccagc gtctctttct catgggccag cagggatttt tcctgctcag
     2161 ataactgccg tttcgtggcg gcctctttca ggaccacata ctgatt
//
LOCUS       DAZGVQ010000317         2181 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000317 DAZGVQ010000000
VERSION     DAZGVQ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.320"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            194..2179
                     /gene="yjcS"
                     /locus_tag="ACHLAG_005238"
     CDS             194..2179
                     /gene="yjcS"
                     /locus_tag="ACHLAG_005238"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFP0658292.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT          585 a          576 c          525 g          495 t
ORIGIN      
        1 ctcatattat ttacagagat aataaattcg cgtaattttc ccccttcagc acaaatgcct
       61 aataaaatgg atcgttccgc tcacacttcc acttattaaa gtcgcaaatt cccaattatg
      121 ataaccccag ctttaaatga cgtattttgc atggattaca tatttacgac ataaatgata
      181 aatggagatt tatatgaata actctcggtt attccgtttg agcagaattg ttattgcgtt
      241 aactgccgcc agcggcatga tggtaaatac cgcttacgca acagatgaag cgaaagccgc
      301 cactcaatat acccaacagg ttaatcagaa ttacgccaaa tcattaccgt ttagcgatcg
      361 tcaggatttt gacgacgctc agcgtggatt tatcgctccg ctgctggatg aaggtattct
      421 gcgcgatgct aatggcaaac catattatcg cggggaagat tataaatttg atatcaatgc
      481 gcccgcgccc gaaaccgtta accctagcct gtggcgtcag tcgcagttaa acggtatttc
      541 cggcctgttt aaagtcaccg acagaatgta ccaggttcgc ggtcaggata tctccaacat
      601 cacctttatc gaaggtgata ccgggattat tgtcatcgac ccgctggtga cgccaccgag
      661 cgcaaaagcc gcccttgacc tttacttcca aaatcgcccg caaaaaccga ttgttgcggt
      721 tatttatacc cacagccata ccgaccacta tggcggcgtg aaaggcatta tctctgaagc
      781 cgatgtgaaa tccggtaaag tgcaggttat cgcccctgct ggctttatgg acgaagccat
      841 cagcgaaaac gtactggcgg gtaatattat gagccgccgt gcactttact cctacggcct
      901 gctgctggcg cataaccctc agggtaacat cggcaatggt cttggcgtaa cgctggcatc
      961 gggctacccg agcatcatcg caccgaacaa aaccatcacc aaaaccggtg agaagatgat
     1021 tatcgacggc ctggagtttg acttcctgat gaccccaggt agcgaagcgc cagccgaaat
     1081 gcacttctat attccggccc tgaaagccct gtgtaccgcc gagaacgcca cgcataccct
     1141 gcacaacttc tacactctgc gcggcgcgaa aacccgcgac accagcaagt ggaccgagta
     1201 tctgaacgaa acgctggata tgtggggtaa cgacgcggaa gtcctgttta tgccgcacac
     1261 ctggccggtc tggggcaata agcatatcaa tgattatatt ggtaaatatc gcgatactat
     1321 caagtacatt cacgaccaga ccctgcacct ggcgaaccag ggctacacca tgaatgaaat
     1381 cggcgacatg attaaactgc cgcctgcact tgccaataac tgggccagcc gtggctatta
     1441 cggttctgtc agccacaacg cccgcgcggt gtataacttc tatcttggct attacgacgg
     1501 taacccggct aacctgcatc cgtatggtca ggtggagatg ggtaaacgtt acgtgcaggc
     1561 gctgggcggt tctgcccgtg tcatcaacct ggcgcaagaa gcgaacaagc aaggtgatta
     1621 ccgctggtcg gcagaactgc tgaaacaggt gattgccgcc aacccgggtg accaggtcgc
     1681 gaagaatctg caagcgaata actttgaaca gctgggctat caggccgagt ccgccacctg
     1741 gcgcggtttc tacctgaccg gcgcgaaaga gctgcgcgaa ggggtgcata agttcagcca
     1801 cggcaccacc ggttccccgg acaccattcg cgggatgtcg gtcgaaatgc tgttcgactt
     1861 tatgtccgtt cgcctcgata gcgcgaaagc cgcgggtaaa aatatcagcc tgaacttcaa
     1921 tatgagcaat ggcgataacc tcaacctgac gctgaacgat agcgtgctta actaccgtaa
     1981 aacactgcaa tcccaagctg acgcctcttt ctacatcagc cgtgaagatc tgcacgccgt
     2041 gctgaccggg caagccaaaa tggcggatct ggtaaaagcg aagaaagcca aaattattgg
     2101 caatggcgcg aaactggaag aaattatcgc ctgtctggat aatttcgatt tgtgggtgaa
     2161 tatcgtaacc ccaaattaat g
//
LOCUS       DAZGVQ010000318         2158 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000318 DAZGVQ010000000
VERSION     DAZGVQ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.321"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1807
                     /locus_tag="ACHLAG_005239"
                     /pseudo
     CDS             1..1807
                     /locus_tag="ACHLAG_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1781..>2158
                     /locus_tag="ACHLAG_005240"
     CDS             1781..>2158
                     /locus_tag="ACHLAG_005240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0658293.1"
                     /translation="MNGIDPLGLSPADVALIRRKDQLNHQRAWDILSDTYEDMKRLNL
                     GGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNLFGMYGRKVKLSHSEM
                     IEDNKKDLAVNDHGLTCPSTTDCS"
BASE COUNT          537 a          557 c          702 g          362 t
ORIGIN      
        1 cacagcgact tcagctacga tgcgctggac cggttggtac agcagggcgg ctttgacggg
       61 cggacgcaac gttatcatta tgacctgacc ggaaaactca cgcagagtga agatgagggg
      121 cttgtcaccc tctggcacta cgatgcgtcg gaccgcatca cgcaccggac ggtgaacggc
      181 gacccggcag agcagtggca gtatgacggc cacggctggc tgagagaaat cagccacctg
      241 agcgaaggcc accgtgtcgc tgtccactac ggctacgacg ataagggccg cctgacgggc
      301 gaacggcaga cggtggagaa cccggagacg ggggaactgc tgtggcagca tgagacgaaa
      361 cacgcctaca acgaacaggg gctggcaaac cgcgtcacgc cggacagcct gccgccggtg
      421 gagtggctga cctacggcag cggttacctg gcgggcatga agctgggcgg gacgccgctg
      481 gtcgagtata cgcgggacag gctgcaccgt gagacggtgc gcagcttcgg cagcatggca
      541 ggcagtaatg ccgcatatga actgaccagc acatacaccc ccgcaggcca gttacagagc
      601 cagcacctga acagcctggt gtatgaccgt gactacgggt ggaatgacaa cggcgacctg
      661 gtgcgcatca gcggtccgcg acagacgcgg gaatacggct acagcgccac gggcaggctg
      721 gagagcgtgc gcaccctcgc accggacctg gacatccgca tcccgtatgc caccgacccg
      781 gcgggaaacc gcctgccgga cccggagcta cacccggaca gcacgctcac ggcgtggccg
      841 gataaccgca tcgcagagga tgcgcactat gtttaccact atgatgaata cggcagactg
      901 acggagaaga cggaccgtat cccgacgggt gtgatacgga tggacgacga gcggacccac
      961 cactaccact acgacagcca gcaccgcctg gtgttccaca cgcggataca gcatggcgag
     1021 ccactggtcg agagccgcta cctctacgac ccgctgggac ggcgaatggc gaaacgggta
     1081 tggcggcggg agcgtgacct gacggggtgg atgtcgctgt cgcgtaaacc ggaggagaca
     1141 tggtacggct gggacggcga caggctgacg acggtacaga ccgacaccac gcgtatccag
     1201 acggtatacc agccggggag cttcgcgccg ctcatccgca tcgaaacaga caacggtgaa
     1261 cgggagaaag cgcagtgccg cagcctggcg gagaagttac agcaggaagg gagcgaagac
     1321 ggtcacggtg tggtgttccc ggctgaactg gtggggctgc tggacaggct ggagggggaa
     1381 atccgggcaa actgcgtgag cagtgaaagc cgccagtggc ttgcgcagtg cgggctgacg
     1441 gtggagcggc tggcggcaca gatagagccg gtttatttgc ctgagcgaaa aatacatctt
     1501 tatcactgtg accaccgggg cctgccgctg gcgcttatca gtgaagacgg caatacggcg
     1561 tggagcgcgg aatatgatga atggggcaac cagcttaatg aggagaaccc gcatcacctg
     1621 caccagccgt accggctgcc ggggcagcag tatgataagg agtcggggct gtactacaac
     1681 cggaaccggt actacgatcc gttgcagggg cggtatatca ctcaggaccc gatagggctg
     1741 gaggggggga tggagtctgt atgcgtaccc gctgaatccg gtgaatggta ttgatccatt
     1801 agggttaagt cccgcagatg tagcgctaat aagaagaaaa gatcaactaa accatcaaag
     1861 agcatgggat atattatctg atacttatga agatatgaag agattaaatt taggtgggac
     1921 tgatcaattt ttccattgta tggcattttg tcgagtgtct aaattaaatg acgctggtgt
     1981 tagccgatcg gcgaaagggc tgggttatga aaaagagatt agagattacg ggttaaatct
     2041 gttcggtatg tacggcagaa aagtaaagct atcccattct gaaatgattg aagataataa
     2101 aaaagacttg gctgtaaatg accatgggtt gacatgtcca tcaacaacag attgctca
//
LOCUS       DAZGVQ010000319         2087 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000319 DAZGVQ010000000
VERSION     DAZGVQ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.322"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..317)
                     /locus_tag="ACHLAG_005241"
     CDS             complement(<1..317)
                     /locus_tag="ACHLAG_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFP0658294.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHAR"
     gene            complement(332..1048)
                     /locus_tag="ACHLAG_005242"
     CDS             complement(332..1048)
                     /locus_tag="ACHLAG_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFP0658295.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            complement(1109..1453)
                     /locus_tag="ACHLAG_005243"
     CDS             complement(1109..1453)
                     /locus_tag="ACHLAG_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFP0658296.1"
                     /translation="MTEADLYPHLAHLASGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYARTITEARQIRQAAREAVLVLAPESVSEMQDYIPENR
                     CHRATMEFQITV"
     gene            complement(1450..1896)
                     /locus_tag="ACHLAG_005244"
     CDS             complement(1450..1896)
                     /locus_tag="ACHLAG_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFP0658297.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(1893..>2087)
                     /locus_tag="ACHLAG_005245"
     CDS             complement(1893..>2087)
                     /locus_tag="ACHLAG_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HFP0658298.1"
                     /translation="GAEIAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGL
                     PVPDATGGRLEILCRLGGEK"
BASE COUNT          460 a          686 c          504 g          437 t
ORIGIN      
        1 cgggcatgta ccggaccata gacggccagc acctgctcac ggtcgtccgg agtgaacacc
       61 cgttgcagat ccgtatcaca caggacatcg cagaacagcg tcacatccgc ttccaggtta
      121 cggcgggttt tcgccaccac cgacagggta tcgtcatcct ctccatcacc attgagcact
      181 tcctgccaca gataccaggc ctctgccgaa ggctcccgca gcaccacgct gacattaccc
      241 cattccggca ccttcaccgt tttatgacga aaccctgaca gtctggccag cgccagcgtt
      301 ttcagatcct ttttcatgat gacccatccc cttatccggc ggctgcgctc actgtcacgg
      361 tgcattcaac agacgtcaca ctctgtgctt tctctgccga atcggtcacc acgcaggtat
      421 atttccccgc atcagcggac tgcgcacctg gcttactgaa ggtgtctgtc gtctgcccgt
      481 caaccggctg accatccttc ttccaggcgt atttatacgg cggcgttccc ccgttggcac
      541 tgactgacat tgtcagcagc gcaccggtat tcacggtaag tgtcttatcc agatttttca
      601 caaacgccag cggtaccaca aaggacaccg gtttgccttt cagacgcagt gagaacgttg
      661 cagccaccac gccgttggta ccggatgacc aggtgtgctg acgcacttcc gccaggaatt
      721 taaagccctt accggacgga aacagcacct taaacgcata caacgcgtca ttgtcataag
      781 catcacgcag ggcgttctgg gcctgattca gataaaaatt acccgacatg gaaatctcgg
      841 aagacgcccc cagaccgttg atgttctcct gctctgtgga gcagagcgtg gtcacatcaa
      901 tatcctgttt ctgaccggcg gtgaactgga cttccttgat ggtgcagtcc aggcgcagat
      961 actccgcctt atccatggtt tcagccgtgg ccggagcaga tgaaatcatc acctgcgtca
     1021 gttgtgaacg ttcatacaaa gcagacattc tgcctcctga taacaaaaaa cccgcacgcg
     1081 gcgggtcagg gtttatgtag aaaaaagatc acaccgtgat ctgaaactcc atggttgcac
     1141 ggtgacagcg gttttccgga atatagtcct gcatttcact gactgattcc ggagccagta
     1201 ccagtacggc ctcacgggca gcctgacgta tctgacgggc ctccgtgatt gtcctagcat
     1261 aaacgtcaat ctgtaccgac accgatgact ccgcctgccc gcccatcacg tccgcagaca
     1321 ccgatgaaat caggctgaaa accacccacg gaagcgcaac cgacggcctg ccatccagca
     1381 gggggaccac atacgggtac acctgcccgc tggcaagatg cgccagatga ggatacaaat
     1441 ccgcctccgt catcgtctca gtacctcatc aatggcccgg ttcatccgcg caatcgccac
     1501 ctgcgctgcc tgttcactgc gcacatcaaa cgccgggcgc acaaacgggt gcggtggcat
     1561 attcacggtt cccatttcca caaaccgcca gtagaaagca ttgcgcgggt tatccgcctt
     1621 catggtgtta tcgctgttac cggtgtccgg attaacacca cggatatgga caccggactc
     1681 catcccgcca tcgcgggagc gccgggaaag gaccaccaca ttgcggcgca gttttcccct
     1741 gcgcaccggt gcccgtgaca ccacttcttc tttcagcaca ttcgcacccg cacgggttgc
     1801 ctcacgcagc acccggttat tttccgcacc actcagaagc tgcaaatcgc ggctgatgtc
     1861 ctccagcccc gaaaaatcca gcagggtttc gatcattttt cccctcccag ccgacagaga
     1921 atttccagac gcccgccggt cgcatccggc acgggcagcc cgacaacgtt caggatccgg
     1981 tcacgccatg gaccactcag cacatgaagt cgtgacgctg ccgtgatttc ccgaccggac
     2041 tgaccgcgca cccagatgcg gatttccgcc tgcgctattt ccgcacc
//
LOCUS       DAZGVQ010000320         2075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000320 DAZGVQ010000000
VERSION     DAZGVQ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.323"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            16..>2075
                     /locus_tag="ACHLAG_005246"
     CDS             16..>2075
                     /locus_tag="ACHLAG_005246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP0658299.1"
                     /translation="MSDRNAQLAYPWNAPLPVIDPETFGRAQPTALRQAIQSYINEDI
                     RIDARLDEETVSFLTNTDEGKRINSHLHHDEERRVRLEKLAKHKRENPPTVVSEAMAE
                     LRALPSFLGNVLIRDLARLNRKQQSARNEGVKNNNTHVADNFVRRGLRTRLKRIEQVN
                     ECFATTAFKATAARHRLDELLMLPQLNRDQIQRLATLTAAAFSTELERICDEVTERTG
                     KGDDNLFTWLLTYQQLARMAIKLGVNPPYWPSLEIRRDRRTAPDPERVPGAVMRITCA
                     TWWNNQLRHLADLWREELLRAAGRVSRKASPYISHESLQEFREKRQRTRDFLKSWDIE
                     NEDGERLSLEDVYWSGLGNPRNRRNEMMACVRGMEQVAESRGDSAFFVTVTCPSRFHS
                     VNEDGSLNQKYNGATVRDASDYLVYDVFAAARKKLNKEGLSWYGVRTVEPHHDGTPHW
                     HMLVFTAPDNEERIIEIMRDAAIKSDRAELGNDISPRFKCEKIDPAKGTPASYIATYI
                     GKNLDASTFHDNDPKTGKPYADKESGKTMAETVENAIAWASLHRIRQFQFFGIPPRQV
                     WRELRRLAGQMERNPASPKRLDHDDIDAILAAADVGCFATYINRQGGVLIPRNLYLIR
                     TAYETADEANDYGEFPQRIYGICAPSLGERYTICTHPDEWKLVRKEAKADNSTDEDFD
                     VGGG"
BASE COUNT          543 a          623 c          553 g          356 t
ORIGIN      
        1 cggggagtac gacgcatgag tgaccgcaat gcacagctgg cgtacccgtg gaacgcaccg
       61 ctaccggtta tcgatcctga aacgttcggc agagcacaac caaccgcatt acgccaggcc
      121 attcagagct acatcaacga ggacattcgc attgatgcca gactggatga agaaaccgtc
      181 tccttcctga caaacacaga cgaaggcaag cgaattaatt cgcatcttca ccacgacgaa
      241 gagcgtcggg tcagactgga aaaactggcg aagcacaaac gcgagaatcc gcccacggtg
      301 gtatcggaag ccatggcaga acttcgcgcc ctgccctcgt ttcttggcaa cgtgctcatt
      361 cgtgatctgg cgcgtttaaa ccgcaaacaa caaagcgccc gcaatgaagg cgtgaaaaac
      421 aacaacactc acgtggccga taacttcgtt cgccgtggac tgcgcacacg cctgaaacgc
      481 atcgaacagg tgaatgaatg cttcgccacg acggcattca aagccaccgc agcgcgtcat
      541 cgccttgatg aattactcat gctgccacaa ctcaaccgtg atcaaattca gcgccttgcc
      601 acactaacgg cggcggcatt ttccaccgaa ctggaacgca tatgtgatga agttactgag
      661 cggacaggaa aaggcgacga taacctgttt acctggttgc tgacttacca gcagctggcg
      721 cgcatggcaa taaaactggg cgtgaaccca ccatactggc cgtcactcga gatcagacgt
      781 gaccgccgca cggcaccaga ccctgaacgg gtaccgggcg cggtaatgcg tatcacctgc
      841 gccacctggt ggaacaacca gctgcgccac ctcgctgatc tctggcggga ggaattactg
      901 cgtgccgccg gaagagtatc gcgcaaggca tccccctaca tcagccatga gtcactccag
      961 gaattccggg agaaacgcca gcgcacccgt gattttctca aaagctggga cattgaaaac
     1021 gaagacggtg aacgcctgag cctggaggac gtgtactggt cagggctggg gaatccgcgc
     1081 aaccgccgca acgagatgat ggcctgcgtc agagggatgg aacaggtcgc cgaatcgcgt
     1141 ggtgactccg cgttcttcgt gacagtgacc tgcccttccc gctttcacag cgtaaacgag
     1201 gacggcagcc tgaaccagaa atacaacggt gccaccgtgc gcgatgccag tgattatctg
     1261 gtttacgacg tctttgcagc tgcaagaaaa aaactcaaca aagaaggcct gagctggtac
     1321 ggggtgcgca ccgttgagcc acaccatgac ggcacaccgc actggcacat gctggtattc
     1381 accgccccgg acaatgaaga acgcatcatt gaaatcatgc gtgacgcagc aatcaaatca
     1441 gatcgcgcag aactggggaa tgatatttct ccgcgcttca agtgcgaaaa aatcgacccg
     1501 gcaaaaggca caccggcaag ctacatcgca acctatattg gcaagaacct ggacgccagc
     1561 acctttcacg acaatgaccc gaaaaccggc aaaccttacg ccgataaaga atcgggaaaa
     1621 accatggcgg aaaccgtgga aaacgccatt gcctgggcga gtctccaccg catccggcag
     1681 ttccagttct tcggtatccc gccccgccag gtatggcgtg aactccgccg ccttgccgga
     1741 caaatggagc gtaaccccgc atcaccgaag cgcctggacc atgacgacat tgacgccatt
     1801 ctggccgccg ctgacgtggg gtgttttgcc acctacatca accgtcaggg cggcgtgctc
     1861 attccacgca acctgtacct gatccgcacc gcatacgaaa ccgcagatga ggccaacgac
     1921 tacggcgaat tcccgcagcg catctacgga atttgtgccc cgtcgctcgg cgagcgctac
     1981 accatctgca cccatccgga cgagtggaaa ctggtcagaa aagaagcgaa agccgacaac
     2041 agcacggacg aggattttga cgttgggggc ggctt
//
LOCUS       DAZGVQ010000321         2011 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000321 DAZGVQ010000000
VERSION     DAZGVQ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.324"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..300)
                     /locus_tag="ACHLAG_005247"
     CDS             complement(<1..300)
                     /locus_tag="ACHLAG_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001310326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658300.1"
                     /translation="MSTQHKNRLPALVVSGYISAAPHKTGAGISTLLTSKAHYRASGF
                     FVRTVLPRFFRIRIMAGRTGPTSVGPGSLLAGCGNPVRLATPSFPPPVGEFSKLTS"
     gene            complement(808..1158)
                     /locus_tag="ACHLAG_005248"
     CDS             complement(808..1158)
                     /locus_tag="ACHLAG_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP0658301.1"
                     /translation="MAVIYIAGPMTGCKDHNRTAFCVEDLKLRCAGNIVLNPAVLPDG
                     LSQQQYMSICIPMLMCADAIYLLEGWVDSAGARAEYVMALKLNMPVSFPENRAAGETI
                     AQLLHKSKAAQATE"
     gene            complement(1187..>2011)
                     /locus_tag="ACHLAG_005249"
     CDS             complement(1187..>2011)
                     /locus_tag="ACHLAG_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFP0658302.1"
                     /translation="GASVFMNTSYKRYILGDSNGALINFYRQARDNTDSLIDEAKTLF
                     LNHNNRGDYLIIRETFNAENHAFLNSRTGISDRRELRLAAMFLYLNRHSFNGLFRVNG
                     KGDFNVPFGCYRKPYFPEREIRAFADKANSTRTLLIHADFKDTLNSASHLFGMGNTLC
                     VYCDPPYLRFSGRDNFTAYGKPFDEDEHVRLRAALDRLSQETGGMTSITISNSDTPET
                     RRIYQGYRMNAIQAPRSVGARTRESAPEVIATLRCITSALSTVRTSAPRTWKKYAC"
BASE COUNT          417 a          507 c          565 g          522 t
ORIGIN      
        1 gctagtaagt tttgaaaact caccaaccgg aggtggaaaa ctcggggtgg cgagacgtac
       61 agggtttcca caaccggcta gcaaagaacc cggcccgacc gaagtcggcc ccgtacgccc
      121 cgccataatt ctgatgcgaa aaaaacgtgg caatacagta cgcacaaaaa aaccgctggc
      181 gcggtagtgc gctttgctag tcagcagggt ggaaatcccg gcacccgttt tatgaggtgc
      241 agcggaaatg taacccgaca cgacgagcgc tggcaagcgg tttttgtgtt gcgtgctgat
      301 gtggttagaa tgtggcgaat taatatcacc tggcaactgg tgattgtctg catacgcggg
      361 gtgtttctga tgagtgataa gctcccttct gattacgaaa ttctttttga cctgtggtac
      421 agctactggc ttgaacacat gacaagcctt ctccaccgtc gcgcttcctt ggttctgagg
      481 attagcgctt tccttgcgat tgttgccgca ttcatttttt gcgacagcgc aacgtttgtt
      541 ttcgccagtg tggtgctctt tctggtgtgg caggtcatgg ctgcctctct ttctatcaga
      601 gctgaaaagg ctgaatggca ggcgcttagc tatcaaagac tgattagcca gcgccacgtg
      661 ctcactccgg aatgcatgat tgcgcgcctc tcctttgttg aggaaagtga cagccccgtg
      721 atggagtgca tgatcaacct tgcctggaat aaagcctgtg aggcggttgg cagctcttcg
      781 cgcaatcatg tttcgatctc cggtcggtta ctcagtcgcc tgtgcggctt ttgatttgtg
      841 aagcagctgc gcgatggttt ctccggctgc ccggttttcc gggaatgaca ctggcatgtt
      901 cagtttgagt gccattacgt actccgcccg cgcgccagct gaatccaccc agccttccag
      961 taagtaaatg gcatcggcac acatgagcat cggaatacaa atgctcatgt actgctgctg
     1021 gcttaatccg tcaggcagta cggcaggatt aagaacgatg tttccggcgc accgaagttt
     1081 caggtcctcc acgcaaaatg ccgtcctgtt gtggtctttg catccggtca tgggaccggc
     1141 gatataaatc actgccatgc tttacctccc gtattgcgtg ttgctgtcag cacgcgtact
     1201 tcttccatgt tcgtggcgca gaagtgcgaa ccgttgataa ggcggatgtg atgcatctca
     1261 gggtggcgat aacctccggt gctgattccc gggttcttgc gccaacgctt cgtggtgcct
     1321 ggatggcgtt catccggtaa ccctgataaa tgcgtctggt ttccggtgtg tcggaattgg
     1381 atatggtgat gctggtcatg ccgcccgttt cctgtgaaag gcggtcaagc gctgccctca
     1441 gacgaacgtg ctcgtcttca tcgaatggtt ttccgtaggc ggtgaaattg tcccggccac
     1501 tgaatcgcag gtaaggggga tcacagtaca cgcaaagcgt gttgcccatg ccgaacaggt
     1561 gactggcaga gttcagcgta tccttaaaat ctgcgtggat aagcagcgtt cgggtgctgt
     1621 ttgccttgtc tgcaaatgcg cggatttccc gctcagggaa gtagggcttg cggtagcagc
     1681 cgaaaggcac gttaaaatcg ccttttccgt tcacgcggaa caaaccgtta aagctgtgtc
     1741 ggttcaggta caaaaacatg gcggccagtc gcagttcacg gcggtcgctt attcccgttc
     1801 tgctgttcag gaaggcatga ttctctgcgt taaaggtttc gcgaatgatg agatagtcac
     1861 ccctgttgtt gtggttcagg aacagggttt tcgcctcgtc gatcaggctg tcggtgttgt
     1921 ccctggcttg ccggtagaaa ttaatcagcg caccattgga atcacccagg atgtaacgct
     1981 tgtaactggt gttcatgaat accgatgcgc c
//
LOCUS       DAZGVQ010000322         2008 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000322 DAZGVQ010000000
VERSION     DAZGVQ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.325"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..225
                     /locus_tag="ACHLAG_005250"
     CDS             <1..225
                     /locus_tag="ACHLAG_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658303.1"
                     /translation="RINTDRSAFIVAVRPRRAISEPEPLTPVGKIMQAYHEKQTRKLN
                     EELARTLAVVNRKAAIVRYVHSAASRQSRR"
     gene            235..444
                     /locus_tag="ACHLAG_005251"
     CDS             235..444
                     /locus_tag="ACHLAG_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658304.1"
                     /translation="MNMTRNSKPKEHTMNDIRRISDKELTKMIESAQQLLSRRNPVVE
                     ETWWENLLAALTELRERRNNEGAAK"
     gene            441..779
                     /locus_tag="ACHLAG_005252"
     CDS             441..779
                     /locus_tag="ACHLAG_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP0658305.1"
                     /translation="MKTPRMTGKVIILRYLETHARFSAPELVEKLGVKRTIINQAAGK
                     MVREGLLVVDGYTDKITHYRKPTEKERLELAQRAEWQQEPSVIEACKRSEIMKRILFI
                     YGAGEELPVI"
     gene            812..1129
                     /locus_tag="ACHLAG_005253"
     CDS             812..1129
                     /locus_tag="ACHLAG_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP0658306.1"
                     /translation="MPTQVATEGRLAADPNTRRTTKGTNVTTAQLAVSLPCHGAEQPT
                     TMWLYLIAFGKQHEELAKHRKGDLILVHGNLQVNRYQDKYGQQKEGWQLLAQSIISSR
                     TGR"
     gene            complement(1179..1715)
                     /locus_tag="ACHLAG_005254"
     CDS             complement(1179..1715)
                     /locus_tag="ACHLAG_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658307.1"
                     /translation="MKNYSYPYLTFVTLSVLGGIFLFIWWRNDVYRITYLYSFTTNCL
                     VSVSVVLSVFVSLCLLKKNLVDRSKFTGVTSYLKFFSGMCLLTWMILFTLLVTLIYFL
                     PGEDSFYSAPYEYSRGSSKSCSGAFVDDPDLQKTIFICYPYGDYQDGNVIYVKKRVNA
                     LGAVVTYAYATSGRFRFD"
BASE COUNT          667 a          500 c          491 g          350 t
ORIGIN      
        1 agaatcaaca ccgacagaag cgcattcatc gttgccgtgc gccctcgtcg cgccatatca
       61 gagcctgaac cactgactcc cgtaggaaaa atcatgcagg cataccacga aaaacagacc
      121 agaaaactca acgaagaact ggcaagaacg ctggccgtcg ttaaccgaaa agcggcaatt
      181 gtccgctatg tgcattcggc cgccagcagg caatcacgca ggtaacaaaa gaccatgaac
      241 atgaccagaa acagcaaacc gaaggagcac accatgaacg acatcaggag gatcagcgac
      301 aaagagctta cgaaaatgat tgagtcggcg cagcagctgc tttcacgacg aaatccggtt
      361 gtggaagaaa catggtggga aaatttactg gcagcactga ccgaactccg cgaacgccgg
      421 aacaacgaag gagccgccaa atgaagacac ccagaatgac cggaaaggta atcattttac
      481 gctacctgga aacgcacgca cgcttcagcg caccagagct ggttgaaaaa ctgggcgtga
      541 aaaggaccat cattaatcag gccgccggga agatggtacg cgaagggctg ctggttgtgg
      601 acgggtacac ggacaagata acgcattacc ggaagccaac ggaaaaggaa cgactggagc
      661 tggcgcaacg ggcagaatgg caacaagagc cgtccgtaat tgaagcctgc aagcgcagcg
      721 aaatcatgaa gcgaattctg tttatctacg gcgctggcga agagctgcca gtcatctaac
      781 cccaacaata aaaccaacac gggagacaaa tatgccaaca caggttgcaa cagagggacg
      841 actggcagca gacccgaaca caaggagaac aaccaaagga accaacgtca caacggcaca
      901 actggcggta tcactaccct gccatggcgc agaacagcca accacaatgt ggctttacct
      961 tattgcattc gggaagcagc acgaagaact ggcaaaacac agaaaaggcg atctgattct
     1021 ggtgcacgga aacttgcagg taaacaggta tcaggacaag tacgggcaac agaaagaggg
     1081 ctggcagttg ctggcgcaaa gcattatcag ctcaagaacc gggcgatgac ttcacgcatg
     1141 ttaccctcag cagcgagggt aacacaggat ttcttttttc agtcaaaccg aaatcggccg
     1201 cttgtcgcgt atgcataggt gaccaccgca cccagcgcat tcacacgctt cttcacataa
     1261 atcacattac catcctgata gtcaccgtat gggtagcaaa taaagattgt tttttgcaaa
     1321 tcaggatcat caacaaatgc ccctgaacaa ctttttgagc taccgcgact gtactcatag
     1381 ggagcggaat agaatgaatc ttcacccgga agaaaatata tcagcgtaac caggagggta
     1441 aacagaatca tccaggtaag aaggcacata cccgaaaaaa acttaagata cgatgtaacc
     1501 ccggtaaatt tagaccgatc aaccaggttc tttttcaaca ggcacagtga gacaaaaacc
     1561 gaaagcacca cagacactga aacaaggcag tttgtcgtga acgaataaag gtaggtaatt
     1621 ctgtaaacat cgtttcgcca ccaaataaaa agaaaaatcc cccccaaaac agaaagcgtg
     1681 acaaaagtta aataaggata tgagtaattt ttcacagttc tcacccggca atacaccact
     1741 gattcagaca gatgattatc atcaccaccc acccccaaaa atcgcaagat aaaaaatgca
     1801 caaaagcgca cgaaattgca caatttttct tatgtgtttc atccccattc agcccagtaa
     1861 cggcgctggc tgaggccggt ttgcacggtg cacgaaaaac gaggcaattg cagcgcgcag
     1921 gcgacgaggg aagggcgccc gaattgaggg gtggaaggaa ggttgcttta tttggggtcg
     1981 tattgcgcgt gatacgcgcg cagtgaag
//
LOCUS       DAZGVQ010000323         1987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000323 DAZGVQ010000000
VERSION     DAZGVQ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.326"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1022
                     /locus_tag="ACHLAG_005255"
     CDS             <1..1022
                     /locus_tag="ACHLAG_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFP0658308.1"
                     /translation="VLGKAGKTGTLYKSLKAIADQVSTRVVVVRVAAAKEGDGQPTQT
                     QLVIGEVKNDGTYTGMQALLVAEQNEAVGVRPRILAVPSLDTKEVVAALVTVAKKLRA
                     FVYAGCPGCNTVQEAVTWRADLAHREVMLIWPDFIAYNPNSGQNEAFPASAYACGLRA
                     KIDHEQGWHKSLSNVAVNNVLGISVPVFWSLQAEDSDAETLNDKEITTLIKRNGFRFW
                     GNRTLDTTTYTFETYTRTAQSLADTIAESQFETVDKPLTPSNVKDVLSDIRAKLSALV
                     TAGKLIGAECWYDVVDNGTTDLRQGIVRIRYRYTPVPPLEDLELHQTFTDEFFDGAFA
                     SLGGE"
     gene            1022..1531
                     /locus_tag="ACHLAG_005256"
     CDS             1022..1531
                     /locus_tag="ACHLAG_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFP0658309.1"
                     /translation="MAVPNKLRVFTVYIDGENKLGRVTSFTPPKLSRKTESFRGAGMP
                     GSVAVDFGLDDGALDLSFSVGGTDSHLFSRYAGALDEVRLRFSGEYYSDADIRYVDIE
                     VRGRITEIDMGEAKQGEDTEHSYSMKNTWYRLSVDDREKIEFDVLNFIWRIDGKDVLP
                     DRTRSMLGF"
     gene            1579..1968
                     /locus_tag="ACHLAG_005257"
     CDS             1579..1968
                     /locus_tag="ACHLAG_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658310.1"
                     /translation="MDKETEMTTDTAVSNGEASSVAVAATAAPKNVVKLTVPVVRGDV
                     SYSEIKIGPLANQAGSLRGLSLLALYNMNCDAMVTWLARVTEPKLKEAEMYAMNTADF
                     TALSSAAVNFFAPSGSNAPAPENTAEP"
BASE COUNT          443 a          469 c          613 g          462 t
ORIGIN      
        1 atgtgctggg taaggccggg aaaaccggca cgctgtacaa aagtctgaaa gctattgccg
       61 atcaggtcag caccagagtt gttgtggtgc gggttgctgc tgcgaaggag ggggatggtc
      121 agccgacaca aacgcagctt gttattggtg aggtcaaaaa tgacggaacc tatacaggca
      181 tgcaggcgct cctggtggct gaacagaatg aggctgttgg tgttcgtccc aggatcctgg
      241 ctgtgccatc gcttgacacc aaagaggtgg tggcggcact ggtaaccgtc gcgaaaaagc
      301 tgcgtgcctt tgtgtatgcg ggctgtcccg gctgtaatac cgtgcaggag gctgtcacct
      361 ggcgcgccga tcttgctcac cgtgaagtta tgctgatctg gcctgatttt atcgcgtaca
      421 accctaacag tggccagaat gaggcttttc ctgcatctgc gtatgcgtgc gggttgcgtg
      481 cgaagattga ccacgagcag ggctggcata aatccctgtc caatgtggca gtgaataatg
      541 tcctgggtat ttctgtgccg gtgttctggt cgctccaggc agaggacagt gacgcggaaa
      601 ccctcaatga caaggagatc accacactca ttaaacgtaa cggcttccgt ttctggggta
      661 accggactct cgacaccact acctacacct ttgagaccta cacgcgtacg gcgcagtcgc
      721 tggcggatac catcgccgaa tcgcagtttg aaaccgtgga caagccgctg acgccgtcga
      781 acgtgaagga tgtgttaagc gatatccggg caaaactgag tgcccttgtg acagcgggta
      841 aacttatcgg ggcagagtgc tggtatgacg tggtggataa cggcacaacg gatctgcgtc
      901 aggggattgt gcggatccgc tacagatata cgccggtacc accgcttgaa gatctggagc
      961 ttcaccagac gtttactgat gagttctttg acggtgcatt tgcatcactg ggaggtgagt
     1021 aatggcggta ccaaacaaac ttcgcgtttt cacggtgtac atcgatggcg aaaacaagct
     1081 ggggcgggtg acgtcgttca cgccgcccaa gctgagtcga aaaacggagt cgttccgtgg
     1141 tgccgggatg cccggcagtg ttgccgttga ctttggtctt gatgatggcg cactggatct
     1201 ttctttttcg gtaggcggta cagactccca ccttttctcc cgttatgccg gggcgcttga
     1261 tgaggtccgt cttcgctttt ccggtgagta ctattcggat gcggatatca gatatgtgga
     1321 tattgaggtc cgcgggcgca tcactgaaat cgatatgggg gaggcgaagc agggtgaaga
     1381 cactgagcac agttattcga tgaaaaacac ctggtacaga ctgagcgtgg atgatcggga
     1441 aaaaatcgaa tttgatgtgc tgaattttat ctggcgcatt gacggtaagg atgtgctgcc
     1501 ggatcgcacc cgctcgatgc tgggcttctg attattgttt aacagagaac ggcggggatc
     1561 cagccgtgga gactgaaaat ggataaagaa acggaaatga ccactgacac tgccgtcagt
     1621 aacggggagg cttcctcggt ggcagttgcc gctacggctg cacctaagaa cgtggtgaag
     1681 ctgacggtgc ctgttgtgcg cggagatgtg tcgtacagtg aaattaaaat cggaccgctg
     1741 gcgaatcagg ccggatcgct gcgtgggctg tctttgcttg cgctttacaa catgaactgt
     1801 gatgccatgg tcacctggct tgcgcgtgtg acagagccaa aacttaagga ggccgaaatg
     1861 tatgccatga atacggcgga ttttacggcg ctgagctcgg cggcggttaa ttttttcgca
     1921 ccctcggggt cgaatgcacc ggcaccggag aacacggcag agccgtaacc tgtctttgct
     1981 ttgactg
//
LOCUS       DAZGVQ010000324         1983 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000324 DAZGVQ010000000
VERSION     DAZGVQ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.327"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..501)
                     /locus_tag="ACHLAG_005258"
     CDS             complement(<1..501)
                     /locus_tag="ACHLAG_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HFP0658311.1"
                     /translation="MTECYIPRSVDAEQAVLGGLMLDNDRWDEVVLMLRGEDFSLAVH
                     RVIFRSMSELAVSGQPFDLITLSEHIEKMHNIEALGGFAYLAELSKNTPSAANIMAYV
                     RIVAEKSMLRQLQETGNSLVADVASPDATPRAVLESAERRLFSLSQTGMLHERSEVSL
                     SSAMELA"
     gene            complement(852..1754)
                     /locus_tag="ACHLAG_005259"
     CDS             complement(852..1754)
                     /locus_tag="ACHLAG_005259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658312.1"
                     /translation="MSSSVKYSPVPAVLTRPELLAELNLLMTRIASGCRVGVTASCRP
                     VIAVHGGYVSYSSPRAGRIFQDGSRPEIVMAVSNRERMCEWFSQVWLPLHLAALEGDE
                     SAFFSAEVNMVRRELQRVPGNSVNCMCWASLAALAFDAEERVELQKGNNENIATYDES
                     IATYAFRAACTVSRCLSRNVSGGIPRSYPEWKAILFPRDGADIFPDGWLENMTRRAER
                     VFTSAKRQCGMLSASQAALFSQQTRQCASEQCSGRIQAGSHHSGSHPDVSAYEVRCAQ
                     EMSLSPVHSTRQCSVQSTDSAETE"
     gene            complement(1739..>1983)
                     /locus_tag="ACHLAG_005260"
     CDS             complement(1739..>1983)
                     /locus_tag="ACHLAG_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000215716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658313.1"
                     /translation="KNSPLKQSVMSMRMLPVPSDRGRQTHRSENVQAVMGILCPATVR
                     PAPFFLFSHSQVSGYLRGSDAPGGQRDMEVKVCLPQ"
BASE COUNT          521 a          558 c          481 g          423 t
ORIGIN      
        1 ggccagctcc attgcggagg aaaggctcac ttcactgcgc tcatgcagca tgcctgtctg
       61 ggaaagagaa aaaagtcgcc gttccgcgga ttcaagaact gcgcgcggag tggcatccgg
      121 cgatgcaaca tcagcaacca gagaattacc ggtttcctga agctgccgca gcatactctt
      181 ttccgccaca atacggacat acgccatgat attggctgct gaaggtgtat tcttactgag
      241 ctcagccagg taagcgaagc cgcccagtgc ctcaatattg tgcattttct caatatgctc
      301 actcagcgtg atgaggtcga acggctgacc gctgactgcc agctctgaca tagagcggaa
      361 tatcacccgg tgcacagcca gagaaaaatc ctcccccctg agcatcagca ccacttcatc
      421 ccagcgatca ttatcaagca tcaggccgcc aagcacagcc tgttcagcat caacggagcg
      481 cggaatatag cattcagtca tggcgtcccc tccctctctg actgccggaa acctggctat
      541 gtgaaaaaag gaaaaagggt gccggacata cagttgtcgg gaataaaatc ccggtaactg
      601 tctgtacgtt ttcagaccgg cgagtttgcc gcccccggtc agaaggtacg gacagcatct
      661 ttatattcat aacactttga cttaataagg aaaaaatatg aatacaagtt atcaacaact
      721 gccgtccggc attggatctg aagtgaaata caccctgcag ttctggctgc tgagtaacgg
      781 aaaggtcatg acggaaaaag acgggctctg taaccccttc cccccgaact gtattgaatc
      841 ggctcatcct ttcactccgt ttcagcagaa tcagtggatt gaactgaaca ctgtcgggtc
      901 gaatggaccg gtgaaagcga catttcttgt gcacagcgta cttcataagc tgaaacgtca
      961 gggtggctcc ctgaatgatg gctgccggcc tgaatacggc cggagcattg ctcactggca
     1021 cactgacgtg tttgttgaga aaaaagcgct gcctgactgg ctgagagcat tccacactga
     1081 cgtttcgcac tcgtaaagac acgttcagcc cgacgggtca tattttccag ccacccgtcg
     1141 gggaaaatgt cagcaccatc acggggaaat aaaatcgctt tccattcagg gtatgagcgc
     1201 ggaatacctc cggaaacatt ccggctcaga cagcgactca cagtacaggc agcacggaat
     1261 gcatatgttg cgatgctctc atcatatgtt gcgatgttct cattattgcc tttctgcagt
     1321 tccacccgct cttcagcatc aaatgccagt gcagcaaggc ttgcccagca catgcagttc
     1381 actgaattcc ccggaacgcg ctgcagctcc cggcgcacca tgttgacctc cgcactgaaa
     1441 aaagcactct catcgccttc aagggcagcc agatgcagcg gaagccagac ctgactgaac
     1501 cattcgcaca tccgttcccg gttactgacg gccatcacta tctccgggcg ggaaccgtcc
     1561 tgaaaaatac gtccggcgcg tggagaactg tatgaaacat aaccaccgtg caccgcaatg
     1621 accggacgac atgacgccgt aacccccaca cgacacccac tggcaatccg ggtcatcagc
     1681 aggttcagtt cagccagcaa ttcagggcgg gttaacaccg ccggaaccgg agaatatttc
     1741 actgaggaag acatactttc acctccatat ctctctgccc gccaggtgcg tcacttcccc
     1801 gcagataacc ggaaacctgg ctatgtgaaa aaaggaaaaa gggcgccgga cgaacagttg
     1861 cagggcataa aatgcccata actgcctgta cattttccga ccggtgagtt tggcgacccc
     1921 ggtcagaagg tactggcagc attcgcatac tcataacaga ttgttttaaa ggagaatttt
     1981 tat
//
LOCUS       DAZGVQ010000325         1964 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000325 DAZGVQ010000000
VERSION     DAZGVQ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.328"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..514
                     /locus_tag="ACHLAG_005261"
     CDS             <1..514
                     /locus_tag="ACHLAG_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFP0658314.1"
                     /translation="KFPLKELNNPEHDSYAMSEKSHGREEIRLHIVCDVPDELIDFTF
                     EWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLH
                     WRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYL
                     ASVLAESGLS"
     gene            complement(556..1326)
                     /gene="yafV"
                     /locus_tag="ACHLAG_005262"
     CDS             complement(556..1326)
                     /gene="yafV"
                     /locus_tag="ACHLAG_005262"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFP0658315.1"
                     /translation="MPGLKITLLQQPLVWMNGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWLALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRLR"
     gene            1507..1953
                     /gene="ivy"
                     /locus_tag="ACHLAG_005263"
     CDS             1507..1953
                     /gene="ivy"
                     /locus_tag="ACHLAG_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFP0658316.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
BASE COUNT          559 a          485 c          481 g          439 t
ORIGIN      
        1 aaaatttccg ctgaaagaat taaataatcc agagcatgac agttacgcaa tgagtgaaaa
       61 gagtcacggc agagaagaaa tccgtcttca tattgtttgc gatgtccctg atgaacttat
      121 tgatttcacg tttgaatgga aaggactgaa gaaattatgc gtggcagtct cctttcggtc
      181 aataatagca gaacaaaaga aagagccaga aatgacggtc agatattata tcagttctgc
      241 tgatttaacc gcagaaaagt tcgccacagc aatccgaaac cactggcacg tggagaataa
      301 gctgcactgg cgtctggacg tggtaatgaa tgaagacgac tgcaaaataa gaagaggaaa
      361 cgccgcagaa ttattttcag ggatacggca catcgctatt aatattttga cgaatgataa
      421 ggtattcaag gcagggttaa gacgtaagat gcgaaaagca gctatggaca gaaactatct
      481 ggcgtcagtc cttgcggaga gcgggctttc gtagtcttac cccgactctc ccccagcctt
      541 aaacacaacc cccactcacc gcaacctaaa ctcatccgca tcccgccatg ccggaaactt
      601 atcgcgatat tcccttaaca ccgccatcga cagctcggca tcaatgcgcg ttgcctgatg
      661 cgcgtcggca gtagcgataa tctcgccttg tggattaatc acccggctgt caccgcgata
      721 atggcagccg ttgccatcgc tgccgacgcg attgcatccc gccacatacg cctgattctc
      781 aatcgcgcgg gccgtcagca atgccagcca gtgcagagag cgcggagcag gccagttggc
      841 gacgtacagg gcgaggtcat aatcgttgag attgcgcgac cacacaggaa aacgtaagtc
      901 gtagcacacc agcggcaaaa tacgccagcc gcgccattcc acaatcactc gcgcattgcc
      961 cgctttataa tgtagatgct catctgccat gcggaacaga tgacgcttat cataaaaatg
     1021 taccgtgccg cccggctcaa ccagcaaaaa gcggttaacc gaaccagact ccgtttgtaa
     1081 tgcaacactg cctgcaatca gcgcattgca ctgctgcgcc ttagctgtca tccagttcac
     1141 tacgtcatct tgtgctagcg acgaagctgc cgcttccatg gcaaagccgc tggtaaacat
     1201 ctccggtaga acgatcacat cgcgcccggt aataccttcc agttgacgat caaaatgacg
     1261 caggttggca gggccattca tccacaccag tggttgctgc aaaagcgtaa tcttcaaacc
     1321 aggcacggtg tacaactcct ttatgcgaag ggttttataa ctttaacacc ttatcaggca
     1381 gttgccttag cgcagaataa attgataaca aatgctgata ttggaaatat ctgatttgca
     1441 aattatcgtg ttatcgccag gctttagaag gttaataaca tgggcaggat aagctcggga
     1501 ggaatgatgt ttaaggcaat aacgacagtc gccgctctgg tcatcgccac cagtgcaatg
     1561 gcgcaggatg atttaaccat tagcagcctt gcaaagggcg aaaccaccaa agctgcattt
     1621 aatcagatgg tacaagggca taagctgcct gcctgggtga tgaaaggcgg tacttatact
     1681 cccgcacaaa ccgtaacgtt gggagatgag acgtatcagg tgatgagcgc gtgcaaaccg
     1741 catgactgtg gctcgcaacg tatcgctgtg atgtggtccg agaaatctaa tcagatgacg
     1801 gggctgttct cgactattga tgagaaaacg tcgcaagaga aactcacctg gctgaatgtg
     1861 aacgatgcgc tttcgattga tggtaaaacg gtgctgttcg cggcgttgac cggcagcctg
     1921 gaaaaccatc cggatggctt taattttaaa taattagcgg ataa
//
LOCUS       DAZGVQ010000326         1942 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000326 DAZGVQ010000000
VERSION     DAZGVQ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.329"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(271..888)
                     /gene="tdk"
                     /locus_tag="ACHLAG_005264"
     CDS             complement(271..888)
                     /gene="tdk"
                     /locus_tag="ACHLAG_005264"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP0658317.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1493..1906
                     /gene="hns"
                     /locus_tag="ACHLAG_005265"
     CDS             1493..1906
                     /gene="hns"
                     /locus_tag="ACHLAG_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFP0658318.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
BASE COUNT          587 a          439 c          392 g          524 t
ORIGIN      
        1 ccttctgggg ccgttttttt gtttacccaa agcaactttt ccataaaccg acagcattag
       61 ccttcatcat atttgcgacg atgtataacg cctaaacaca gggatattgt actttacagg
      121 tcacaagtca acgtcggtgc ttaagagccc tgtgaggcgt atagcggcgt taaaaaactg
      181 ccgagaaggg tatatagccc ggaagaagtg cgtaaaacga actgacagga taaaagtgcc
      241 ctgctcaccc tgtcagtaaa gaaattctta ttaatcgtgg cgatgccttt cctgaatagc
      301 cgttaatgag ccgacttgta acgcctcttt atagtgttta cggcatacag aaacgtatcg
      361 ttcattacca ccaattacca cctgctcacc ttcgttataa ggtctgcctg cttgatcaag
      421 acgcagcacc atgcttgctt tacggccaca aaaacagatg gtttttaatt caaccagttt
      481 gtcggaccat gccagtaagt attggctgcc aataaataat tcacctcgaa aatcggtacg
      541 taaaccataa caaagtacgg gtatatcgag ttgatcgaca acctccgata attcatatac
      601 ttgttgtctg gttaaaaact ggcattcatc aaccagtacg caatgaattg cctgctgttc
      661 atgttccgca cgaatctcat caaataatga tgaattttgg ttaaataatt ttgcaggcga
      721 tgacaaacct atacgcgaac tgactttccc ggcaccaaag cgatcatcaa tttctgccgt
      781 atatacgaca gtgcgcatgc cgcgttcctg gtaattgtat gaagattgca acaatgctgt
      841 agacttaccc gcattcattg cggaatagta gaaatatagc tgtgccatca gccacaggcc
      901 ctcaatgata tgaaataaag ttggctggag tttatcataa ttcgtcagtt tttcagctca
      961 acagtatgca cagaagtggg tatatgcgtt ctcccttacg aagccttgca taatccttct
     1021 gagctatcat tacaactgcc tcgcttgtta taagcgggta aatgactgct ggtaactatt
     1081 cacaatcttt aacctgttgc gcaagtaata gccctctgtt gacctccagg agatagtgca
     1141 atactaagtc catgctctta ttgcgactgt tctacttttc atcattcgct taatagggaa
     1201 ttctcgtaaa cacaactaat acagaagact gaaaggtcgt cagcctacga taatctcccc
     1261 ataaaatgtg acatgaatca ggaagtttta acctcacgtg ctgcgaaatc atcggtgtaa
     1321 atagggctat atgccgcgtc ttttctggct aattttatga aaagatattt attggcggca
     1381 caaaataaag aacaattttg aattccttac attcctggct attgcacaac tgaatttaag
     1441 gctctattat tacctcaaca aaccacccca atataagttt gagattacta caatgagcga
     1501 agcacttaaa attctgaaca acatccgtac tcttcgtgcg caggcaagag aatgtacact
     1561 tgaaacgctg gaagaaatgc tggaaaaatt agaagttgtt gttaacgaac gtcgcgaaga
     1621 agaaagcgcg gctgctgctg aagttgaaga gcgcactcgt aagctgcagc aatatcgcga
     1681 aatgctgatc gctgacggta ttgacccgaa cgaactgctg aatagccttg ctgccgttaa
     1741 atctggcacc aaagctaaac gtgctcagcg tccggcaaaa tatagctacg ttgacgaaaa
     1801 cggcgaaact aaaacctgga ctggccaagg ccgtactcca gctgtaatca aaaaagcaat
     1861 ggatgagcaa ggtaaatccc tcgacgattt cctgatcaag caataatctt ttgtagattg
     1921 cacttgctta aaatcccgcc ag
//
LOCUS       DAZGVQ010000327         1932 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000327 DAZGVQ010000000
VERSION     DAZGVQ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.330"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..216)
                     /locus_tag="ACHLAG_005266"
     CDS             complement(<1..216)
                     /locus_tag="ACHLAG_005266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HFP0658319.1"
                     /translation="MARLSLSREGRDSLERYMIQQASLAGITTERLGKTFSVDPSIQQ
                     KMENAIKESAGLLQEINFIGVDDQEGEK"
     gene            complement(234..1070)
                     /locus_tag="ACHLAG_005267"
     CDS             complement(234..1070)
                     /locus_tag="ACHLAG_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFP0658320.1"
                     /translation="MGSKNTPERKKFRVAVSGSTIDGREISGEMLRQAAENYDPSVYA
                     SRVNIEHVLSMWPSSELCAVGDLIALSTDEITEGKLAGRTALYAEIEPTDRMKNMIAD
                     GKKVYSSIEMLPELDAVKGPYIVGLAMTDTPASLGTERLEFAAQQRASIMQFSKCTTE
                     PPMFTVAMEAEMLTTTEQRTEESRQWFSRVMALISKTRTADGEQFSQLRDVVENIASD
                     HADLIDRFSTLEQKHALDKTALDELSHELAALKAQLRNQDADPQQRFTSAGGTDQTLA
                     EF"
     gene            1226..>1932
                     /locus_tag="ACHLAG_005268"
     CDS             1226..>1932
                     /locus_tag="ACHLAG_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFP0658321.1"
                     /translation="MIQDVFIRMRAKQLYWQGYTPADIAQLMGINQNTIYSWKKRDEW
                     DATPPIQRVTQSIDARLIQLTAKPDKSGGDFKEIDLLTRQLKKLNDGQGEQAVSGKKP
                     RRRKLKNHFTEEQIVALREKILPSLAWHQREWYDNRHHRNRMILKSRQIGATWYFARE
                     ALLDALRDDVSAGYQRNQIFLSASRRQAYQFKHFIQQAALEVDVELKGGDKIVLSNGA
                     ELHFLGTSAATAQSYTG"
BASE COUNT          422 a          508 c          518 g          484 t
ORIGIN      
        1 tttttcacct tcctggtcat ccacaccgat aaaattgatt tcctgtaaca gtccggcgct
       61 ttccttgatg gcgttctcca tcttctgctg aatggatgga tccacgctga aggttttacc
      121 caggcgctct gttgtgatac cagccagcga tgcctgctga atcatatagc gttccagact
      181 gtcacgtcct tcacgggaaa gggataaacg ggccataaat tactcttctc ctgtcagaat
      241 tcagccagtg tctggtcagt gccgccagcg gaagtaaaac gctgctgtgg atcggcatcc
      301 tggtttctca gttgcgcttt cagtgctgca agctcgtgac tgagctcatc cagcgccgtt
      361 ttgtcgaggg catgtttctg ctccagagtg ctgaaacggt cgataagatc tgcatgatcg
      421 gatgcgatgt tttccacaac gtcgcgcagc tggctgaact gctcgccatc cgctgtgcgt
      481 gttttgctga tgagtgccat gacgcggctg aaccactgac ggctctcttc cgttcgctgt
      541 tctgtggtgg taagcatctc tgcttccatg gcgacggtga acatgggggg ttctgttgtg
      601 cacttactga actgcatgat gctggcgcgt tgctgtgcgg caaactccag gcgttcagtg
      661 cccagacttg ctggcgtgtc ggtcattgcc agacccacaa tgtaaggccc cttgacggcg
      721 tccagctcag gcaacatctc aatgctcgaa tacacttttt tcccgtcagc aatcatgttt
      781 ttcatgcggt cggtgggttc aatttcggca tataacgctg tacgccctgc cagtttccct
      841 tcggtgattt catcagttga cagagcgatc agatcaccca ctgcgcagag ttcactggat
      901 ggccacatcg aaaggacgtg ctcaatgttc acccgggagg cgtaaacgga cgggtcatag
      961 ttttctgcgg cctgacgcag catttcacca gaaatttcac gtccgtcgat ggttgaaccg
     1021 gaaacggcaa cgcgaaattt tttgcgctct ggcgtgtttt tgcttcccat ggaaattctc
     1081 cataacctgg tcgtttttcc catgatggcg gcagataacg gtgtgtctca aggtgcttaa
     1141 gttgtatggc gacggggaca acgcgggctg gtatcaggcg gtattgctga ggcttaatct
     1201 tgccctgtta atgtcagggg gtgcaatgat ccaggatgtc tttattcgta tgcgggcgaa
     1261 acagctttac tggcaggggt ataccccggc ggatatcgct cagctgatgg gaataaacca
     1321 gaacacgatt tattcgtgga aaaaacgcga tgagtgggat gccactcccc cgatccagcg
     1381 ggtcacccag tccattgatg cacgcctgat acagctcacc gccaaaccgg ataaaagcgg
     1441 cggtgacttt aaggaaattg acctgctgac gcgccagctg aaaaaactga atgacggtca
     1501 gggagaacag gctgtttccg gtaagaagcc ccgcaggcgc aaactgaaaa atcacttcac
     1561 cgaagagcag attgtcgcac tcagggaaaa aatcctgccg tcactggcct ggcatcagcg
     1621 cgaatggtac gacaaccgcc atcaccgcaa ccgcatgatc ctgaagtcgc gtcagattgg
     1681 tgcgacctgg tattttgcgc gcgaagcact ccttgatgca ctgcgtgatg atgtctccgc
     1741 cgggtaccag cgcaaccaga tatttttgtc cgcatcccgt cgccaggcgt atcagttcaa
     1801 acactttatt cagcaggcgg cgctggaagt tgatgtcgaa ctgaaagggg gtgacaagat
     1861 tgttttgtct aacggtgcag agcttcactt ccttggcaca tcggcagcaa ccgctcagtc
     1921 gtataccgga ca
//
LOCUS       DAZGVQ010000328         1903 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000328 DAZGVQ010000000
VERSION     DAZGVQ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.331"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1903
                     /locus_tag="ACHLAG_005269"
     rRNA            <1..>1903
                     /locus_tag="ACHLAG_005269"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          514 a          417 c          592 g          380 t
ORIGIN      
        1 tgtgtgttag tggaagcgtc tggaaaggcg cgcgatacag ggtgacagcc ccgtacacaa
       61 aaatgcacat gctgtgagct cgatgagtag ggcgggacac gtggtatcct gtctgaatat
      121 ggggggacca tcctccaagg ctaaatactc ctgactgacc gatagtgaac cagtaccgtg
      181 agggaaaggc gaaaagaacc ccggcgaggg gagtgaaaaa gaacctgaaa ccgtgtacgt
      241 acaagcagtg ggagcacgct taggcgtgtg actgcgtacc ttttgtataa tgggtcagcg
      301 acttatattc tgtagcaagg ttaaccgaat aggggagccg aagggaaacc gagtcttaac
      361 tgggcgttaa gttgcagggt atagacccga aacccggtga tctagccatg ggcaggttga
      421 aggttgggta acactaactg gaggaccgaa ccgactaatg ttgaaaaatt agcggatgac
      481 ttgtggctgg gggtgaaagg ccaatcaaac cgggagatag ctggttctcc ccgaaagcta
      541 tttaggtagc gcctcgtgaa ttcatctccg ggggtagagc actgtttcgg caagggggtc
      601 atcccgactt accaacccga tgcaaactgc gaataccgga gaatgttatc acgggagaca
      661 cacggcgggt gctaacgtcc gtcgtgaaga gggaaacaac ccagaccgcc agctaaggtc
      721 ccaaagtcat ggttaagtgg gaaacgatgt gggaaggccc agacagccag gatgttggct
      781 tagaagcagc catcatttaa agaaagcgta atagctcact ggtcgagtcg gcctgcgcgg
      841 aagatgtaac ggggctaaac catgcaccga agctgcggca gcgacactgt gtgttgttgg
      901 gtaggggagc gttctgtaag cctgcgaagg tgtgctgtga ggcatgctgg aggtatcaga
      961 agtgcgaatg ctgacataag taacgataaa gcgggtgaaa agcccgctcg ccggaagacc
     1021 aagggttcct gtccaacgtt aatcggggca gggtgagtcg acccctaagg cgaggccgaa
     1081 aggcgtagtc gatgggaaac aggttaatat tcctgtactt ggtgttactg cgaagggggg
     1141 acggagaagg ctatgttggc cgggcgacgg ttgtcccggt ttaagcgtgt aggctggttt
     1201 tccaggcaaa tccggaaaat caaggctgag gcgtgatgac gaggcactac ggtgctgaag
     1261 caacaaatgc cctgcttcca ggaaaagcct ctaagcatca ggtaacatca aatcgtaccc
     1321 caaaccgaca caggtggtca ggtagagaat accaaggcgc ttgagagaac tcgggtgaag
     1381 gaactaggca aaatggtgcc gtaacttcgg gagaaggcac gctgatacgt aggtgaagtc
     1441 cctcgcggat ggagctgaaa tcagtcgaag ataccagctg gctgcaactg tttattaaaa
     1501 acacagcact gtgcaaacac gaaagtggac gtatacggtg tgacgcctgc ccggtgccgg
     1561 aaggttaatt gatggggtca gcgcaagcga agctcttgat cgaagccccg gtaaacggcg
     1621 gccgtaacta taacggtcct aaggtagcga aattccttgt cgggtaagtt ccgacctgca
     1681 cgaatggcgt aatgatggcc aggctgtctc cacccgagac tcagtgaaat tgaactcgct
     1741 gtgaagatgc agtgtacccg cggcaagacg gaaagacccc gtgaaccttt actatagctt
     1801 gacactgaac attgagcctt gatgtgtagg ataggtggga ggctttgaag tgtggacgcc
     1861 agtctgcatg gagccgacct tgaaatacca ccctttaatg ttt
//
LOCUS       DAZGVQ010000329         1866 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000329 DAZGVQ010000000
VERSION     DAZGVQ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.332"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..900
                     /locus_tag="ACHLAG_005270"
     CDS             <1..900
                     /locus_tag="ACHLAG_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFP0658322.1"
                     /translation="PTQTQLVIGEVKNDGTYTGMQALLVAEQNEAVGVRPRILAVPSL
                     DTKEVVAALVTVAKKLRAFVYAGCPGCNTVQEAVTWRADLAHREVMLIWPDFIAYNPN
                     SGQNEAFPASAYACGVRAKIDHEQGWHKSLSNVAVNNVLGISVPVFWSLQAEDSDAET
                     LNDKEITTLIKRNGFRFWGNRTLDTTTYTFETYTRTAQTLADTIAESQFETVDKPLTP
                     SNVKDVLSDIRAKLSALVTAGKLIGAECWYDVVDNGTTDLRQGIVRIRYKYTPVPPLE
                     DLELHQTFTDEFFAGAFASLGGE"
     gene            900..1409
                     /locus_tag="ACHLAG_005271"
     CDS             900..1409
                     /locus_tag="ACHLAG_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFP0658323.1"
                     /translation="MAVPKKLRVFTVYIDGENKLGQVTSFTPPKLSRKTESFRGAGMP
                     GSVAVDFGLDDGALDLSFSVGGTDSHLFSRYAGALDEVRLRFSGEYYSDAGIEYVDIE
                     VRGRITEIDMGEAKQGEDTGHSYTMKNTWYRLSVDDREKIEFDVLNFIWRIDGKDVLP
                     DRTRSMLGF"
     gene            1458..1847
                     /locus_tag="ACHLAG_005272"
     CDS             1458..1847
                     /locus_tag="ACHLAG_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658324.1"
                     /translation="MDKETEMATGTAVSNGEASSVAVAATAAPKNVVKLTVPVVRGDV
                     SYSEIKIGPLANQAGSLRGLSLLALYNMNCDAMVTWLARVTEPKLKEAEMYAMNTADF
                     TALSSAAVNFFAPSGSNAPAPENTAEP"
BASE COUNT          416 a          443 c          569 g          438 t
ORIGIN      
        1 ccgacacaaa cgcagcttgt tattggtgag gtcaaaaatg acggaaccta tacaggcatg
       61 caggcgcttc tggtggctga acagaatgag gctgttggtg ttcgtcccag gatcctggct
      121 gtgccatcgc ttgacaccaa agaggtggtg gcggcactgg taaccgtcgc gaaaaagctg
      181 cgtgcctttg tgtatgcggg ctgtcccggc tgtaataccg tgcaggaggc tgtcacctgg
      241 cgcgccgatc ttgctcaccg tgaagttatg ctgatctggc ctgattttat cgcgtacaac
      301 cctaacagtg gccagaatga ggcttttcct gcatctgcgt atgcgtgcgg ggtgcgtgcg
      361 aagattgacc acgagcaggg ctggcataaa tccctgtcca atgtggcggt gaataatgtc
      421 ctgggtattt ctgtgccggt gttctggtcg ctccaggcag aggacagtga cgcggaaacc
      481 ctcaatgaca aggagatcac cactctcatt aaacgtaacg gcttccgttt ctggggtaac
      541 cggactctcg ataccactac ctacaccttt gagacttaca cgcgtacggc gcagacgctg
      601 gcggatacca tcgccgaatc gcagtttgaa accgtggaca agccgctgac gccgtcgaac
      661 gtgaaggatg tgttaagcga tatccgggca aaactgagtg cccttgtgac agcgggtaaa
      721 cttatcgggg cagagtgctg gtatgacgtg gtggataatg gcacaaccga tctgcgtcag
      781 gggattgtgc gcattcgcta caagtatacg ccggtgccac cgcttgaaga tcttgagctt
      841 caccagacgt ttaccgatga attctttgcg ggtgcatttg catcactggg aggtgagtaa
      901 tggcggtacc aaagaaactt cgtgttttca cggtgtacat cgatggcgaa aacaagctgg
      961 gacaggtgac gtcgttcacg ccgcccaagc tgagtcgaaa aacggagtcg ttccgtggtg
     1021 ccgggatgcc cggcagtgtt gccgttgact ttggtcttga tgatggcgca ctggatcttt
     1081 ctttttcggt aggcggtaca gactcccacc ttttctcccg ttatgccggg gcgcttgatg
     1141 aggtccgtct tcgcttttcc ggtgagtact attcggatgc gggtatcgaa tatgtggata
     1201 tcgaggtgcg cggacgtatc actgaaattg atatgggtga ggcgaagcag ggagaagata
     1261 ctggtcacag ctacacgatg aaaaacacct ggtacagact gagcgtggat gaccgggaaa
     1321 aaatcgaatt tgatgtgctg aattttatct ggcgcattga cggtaaggat gtgctgccgg
     1381 atcgcacccg ctcgatgctg ggcttctgat gatggtttaa cagagaacgg cggggatacc
     1441 gccgtggaga ctgaaaaatg gataaagaaa cggaaatggc cactggcact gccgtcagta
     1501 acggggaggc ttcctcggtg gcagttgccg ctacggctgc acctaagaac gtggtgaagc
     1561 tgacggtgcc tgttgtgcgc ggagatgtgt cgtacagtga aattaaaatc ggaccgctgg
     1621 cgaatcaggc cggatcgctg cgtgggctgt ctttgcttgc gctttacaac atgaactgtg
     1681 atgccatggt cacctggctt gcgcgtgtga cagagccaaa actcaaggag gccgaaatgt
     1741 atgccatgaa tacggcggat tttacggcac tgagctcggc ggcggttaat tttttcgcac
     1801 cctcggggtc gaatgcaccg gcaccggaga acacggcaga gccgtaacct gtctttgctt
     1861 tgactg
//
LOCUS       DAZGVQ010000330         1865 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000330 DAZGVQ010000000
VERSION     DAZGVQ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.333"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            219..413
                     /locus_tag="ACHLAG_005273"
     CDS             219..413
                     /locus_tag="ACHLAG_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658325.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            421..1833
                     /gene="pykF"
                     /locus_tag="ACHLAG_005274"
     CDS             421..1833
                     /gene="pykF"
                     /locus_tag="ACHLAG_005274"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFP0658326.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          504 a          456 c          436 g          469 t
ORIGIN      
        1 ggatgaactt tcacttatcc tcacactgac aacttcggca ccagacgttg cgcaaacagt
       61 gaagtttttg cgtaaccttt tccctggaac gttaaatctt tgataacaat ttattgtcta
      121 acaagttgta tattttttga aacgctgttt ttgttttcct tttggattaa tttcagcgta
      181 taatgcgcgc caattgactc ttgaatggtt tcagcacttt ggactgtaga actcaacgac
      241 tcaaaaacag gcactcacgt tgggctgaga cacaagcaca cattcctctg cacgcttttt
      301 cgatgtcacc tatccttaga gcgaggcacc accactttcg taataccgga ttcgctttcc
      361 ggcagtgcgc ccagaaagca agtttctccc atccttctca acttaaagac taagactgtc
      421 atgaaaaaga ccaaaattgt ttgcaccatc ggaccgaaaa ccgaatctga agagatgtta
      481 gctaaaatgc tggacgctgg catgaacgtt atgcgtctga acttctctca tggtgactat
      541 gcagaacacg gtcagcgcat tcagaatctg cgcaacgtga tgagcaaaac tggtaaaacc
      601 gccgctatcc tgcttgatac caaaggtccg gaaatccgca ccatgaaact ggaaggcggt
      661 aacgacgttt ctctgaaagc tggtcagacc tttactttca ccactgataa atctgttatc
      721 ggcaacagcg aaatggttgc ggtaacgtat gaaggtttca ctactgacct gtcagttggc
      781 aacaccgtac tggttgacga tggtctgatc ggtatggaag ttaccgccat tgaaggtaac
      841 aaagttatct gtaaagtgct gaacaacggt gacctgggcg aaaacaaagg tgtgaacctg
      901 cctggcgttt ccattgctct gccagcactg gctgaaaaag acaaacagga cctgatcttt
      961 ggttgcgaac aaggcgtaga ctttgttgct gcttccttta ttcgtaagcg ttctgacgtt
     1021 atcgaaatcc gtgagcacct gaaagcgcac ggcggcgaaa acatccacat catctccaaa
     1081 atcgaaaacc aggaaggcct caacaacttc gacgaaatcc tcgaagcctc tgacggcatc
     1141 atggttgcgc gtggcgacct gggtgtagaa atcccggtag aagaagttat cttcgcccag
     1201 aagatgatga tcgaaaaatg tatccgtgca cgtaaagtcg ttatcactgc gacccagatg
     1261 ctggattcca tgatcaaaaa cccacgcccg actcgcgcag aagccggtga cgttgcaaac
     1321 gccatcctcg acggtactga tgcagtgatg ctgtctggtg aatccgcaaa aggtaaatac
     1381 ccgctggaag cggtttctat catggcgacc atttgcgaac gtaccgaccg cgtgatgaac
     1441 agccgtctcg agttcaacaa tgacaaccgt aaactgcgca ttaccgaagc ggtatgccgt
     1501 ggtgccgttg aaactgctga aaaactggat gctccgctga tcgtggttgc tactcagggc
     1561 ggtaaatctg ctcgcgcagt acgtaaatac ttcccggatg ccaccatcct ggcactgacc
     1621 accaacgaaa aaacggctca tcagttggta ctgagcaaag gcgttgtgcc gcagctggtt
     1681 aaagagatca cttctactga tgatttctac cgtctgggta aagaactggc tctgcagagc
     1741 ggtctggcac acaaaggtga cgttgtagtt atggtttctg gtgcactggt accgagcggc
     1801 actactaaca ccgcatctgt tcacgtcctg taatattgct tttgtgaatt aatttgtata
     1861 tcgaa
//
LOCUS       DAZGVQ010000331         1828 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000331 DAZGVQ010000000
VERSION     DAZGVQ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.334"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            92..723
                     /gene="yqeH"
                     /locus_tag="ACHLAG_005275"
                     /pseudo
     CDS             92..723
                     /gene="yqeH"
                     /locus_tag="ACHLAG_005275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1057..>1828
                     /gene="yqeI"
                     /locus_tag="ACHLAG_005276"
     CDS             1057..>1828
                     /gene="yqeI"
                     /locus_tag="ACHLAG_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0658327.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVAD"
BASE COUNT          612 a          335 c          340 g          541 t
ORIGIN      
        1 tttcccagca aaattacaaa atcatggtgt attgacatca tcaatcacca atgaaattat
       61 gcaattatat acggataggg aggttcttaa catggggctt tgtagtcgtt ataaaagtct
      121 tacatgcaat agttgctcaa tgcattgcca aataatgcca gaagagtcac cgcgtttgca
      181 gtactgcgca aactcgtgtt tttgtatgtg gcccgaagaa agttcatatt ttaatcgtgg
      241 tgtggtagaa ggtatcttaa caaaaaacca caatgcgaga ttaagcggat atatttttgt
      301 cgatttttca gtaagttttt tacgtctatt ccttgaaaaa gactggattg actatcttgc
      361 aagtactgac atggggattg ttttagtcag cgaccgcaat atgcatcgtt agctaattac
      421 tggcgaaaac acaactcagc aatatctgct gtcatatata atgatgatgg tcttgatgtt
      481 gccaatgaaa agatcagaca actatttatt ggtcgttatt tatcatttac cagaggtaac
      541 acattaactc agatggaatt caccataatg gggtatatgg tttctggtta taatccatat
      601 cagattgctg aagttctgga tatggatatc cgtagcatct acgcgtacaa gcaacgaatc
      661 gaaaagagaa tgggtggtaa aataaacgaa ttatttattc gttcacattc ggtccaacat
      721 tgatactcaa cgaccagcca gaatcatact ctgtttatac gggcatttat tgaaatgtcc
      781 gtattttata aataggccgt cccctcaaga gtaaacacca tagtatttaa aaccatcaca
      841 cgaaaaattc agaagcatta cgaaatatga atttacaaac aatagtggca taaatgttaa
      901 ccatgttaat ttacgtaaag ttttacgttg caacattaaa gcctcatttc aatcatcatg
      961 ataaatataa aattaatata tatttatgcc gtaaatacca atatacttag caaactatgt
     1021 gatctccatt tcgattgatt tagtgtttat tgacgtatgt actggattat taacgataat
     1081 atcgagttct ggcctgagca ccgaaaatta atatcggtac ataacgccga tcttaacgtc
     1141 gttctgacaa cgccagccag tcgatgttta tcacttctac ttgaagcttt tcctgatgtg
     1201 gttgcacaac aagatttttt caccagagtc tgggaagaag aaggtatgcg tgtgcctact
     1261 aacacgttat atcagaacat atccattatc agacgcggat ttcgcgctgt tggtgatact
     1321 acccactcgc taattgcaac cgtgccgaga agaggattca agatccataa tgacattaac
     1381 atacaaaatc atgtaataaa ctcgtcaaca gacgcacata cacacaatgc cccacctgcc
     1441 ataaaagtta atgcgggata caaagagagc attggtggcg caaagaattt caataacaaa
     1501 atcctcaaac atataaaaag tcatctaatt atgttgagcg catttgtcat aggcgcatat
     1561 tctgcatatt ggctatggaa taataatcaa ccgaagccat ttttcaagga ttacaagact
     1621 gttgcggaaa ttaatggctg ccattttaat gtaacagaag atacaattga cgggttgaaa
     1681 gagttcgata aatataaaac acggatactg gattcgggga ttaactgtaa aaaacatccg
     1741 tggttatact tccctcttgc aaaatcctca ccggggatga ttgttatggc atgcaataaa
     1801 aattataacc aacatgaagt ggcagact
//
LOCUS       DAZGVQ010000332         1825 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000332 DAZGVQ010000000
VERSION     DAZGVQ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.335"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1072
                     /gene="chaA"
                     /locus_tag="ACHLAG_005277"
     CDS             <1..1072
                     /gene="chaA"
                     /locus_tag="ACHLAG_005277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFP0658328.1"
                     /translation="RHKETSLIFPVLALVVLFLWGSSQTLPVVIAINLLALIGILSSA
                     FSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTLMRDTLYSIIM
                     IVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMALPAANFSTGQ
                     ALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAHSSVWHAIWLI
                     IHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGALKAVLNNQVQR
                     AMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLVLCHISFSTGR
                     TNVLNGAAHLALFAAYLMTIFA"
     gene            1477..1584
                     /locus_tag="ACHLAG_005278"
     CDS             1477..1584
                     /locus_tag="ACHLAG_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP0658329.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            complement(1599..>1825)
                     /locus_tag="ACHLAG_005279"
     CDS             complement(1599..>1825)
                     /locus_tag="ACHLAG_005279"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658330.1"
                     /translation="GYYDSIHLHFIPEMFAMKSAFRFFNSCYSRFMSTLDDASSSGCQ
                     YLLVEEIKTKMAAGCYPCGRFRAHIAITAA"
BASE COUNT          433 a          432 c          456 g          504 t
ORIGIN      
        1 ccgccacaag gagacttcgc ttattttccc ggttctggcg ctggtagtgc tgttcctgtg
       61 gggaagcagc cagacactac cagtggtcat tgccatcaat cttcttgcgc ttattggtat
      121 tttaagtagc gcctttagtg ttgtccgtca tgcggacgta ttagcccatc gcctgggaga
      181 accttacggt tcgcttattc ttagcctttc agtggttatt cttgaagtca gtttgatttc
      241 agctttaatg gcaaccggcg acgccgcgcc aacgctaatg cgtgatacgc tctattcaat
      301 cattatgatt gttaccggtg ggctggttgg cttttcatta ttgttgggcg gtcgtaagtt
      361 tgccacccaa tatatgaatc tgtttggtat caagcagtat ttaattgcgc tgttccccct
      421 ggcgataatc gtactggtat ttccaatggc tctgcctgcg gcgaattttt caaccggtca
      481 ggcgttactg gtagcattaa tttctgcggc aatgtatggc gtatttttgc tgatccagac
      541 caaaacgcat caaagtttgt ttgtctacga gcacgaagat gacagtgatg acgatgaccc
      601 gcatcacggt aaaccgtctg cccatagcag cgtgtggcat gctatctggt tgattatcca
      661 tctgattgcc gttattgcag tcaccaaaat gaatgccagc ccgctggaga cattgctcga
      721 cagcatgaat gcccctgtcg cctttactgg cttcctggtg gctctgttga ttctgtcgcc
      781 ggaaggttta ggtgcgttaa aagcagtgtt gaacaaccag gtccagcgcg cgatgaatct
      841 gttctttggt tcagtgttag caaccatttc gcttaccgta cctgtcgtca cgctaattgc
      901 ctttatgacg ggtaacgaat tacagtttgc acttggtgcg ccagaaatgg tggtgatggt
      961 ggcctcttta gtgctgtgcc atatctcctt ctccaccgga cgtactaacg tgctcaatgg
     1021 cgcagcgcat ctggcactgt ttgccgccta tttgatgacg atatttgcct gacagttata
     1081 tctcctccga tgacggaaat cgggggagat aagataaatt cctgcttatt gtatttcttc
     1141 atactacctg gacagctcgt taatcagaat tgcgagcctg taccacaacc tgcacgaaac
     1201 cactgcaaca gctgttgaaa agccggaatg tactctttat tgcaaacatt tcagggatga
     1261 agtgaagatg aattgagacg tagtatccac accaagccgg aattatccgg tgaggcgcaa
     1321 tgttgcgggg gctttatccc tggtggcatt ggttgctgga aagagaaaac ccccgcacgt
     1381 tgagaggtaa aacctgacaa caaaacgggg gctaatcttg aaaccagaca cttcaagaat
     1441 agccgcaaaa tgttgtcatt acaacaaggc ggctatatga cgctcgcgca gtttgccatg
     1501 actttctggc acgacctagc agcaccgatc ctggcgggga ttattaccgc agcgattgtc
     1561 agctggtggc gtaaccggaa gtaaggtgaa aacgggcgtc aggctgccgt gatggcaata
     1621 tgcgcccgaa accggccgca ggggtaacag cctgcggcca tttttgtctt tatctcttcc
     1681 acaagtaaat attggcaacc cgagctggaa gcatcatcca gagtactcat gaaacgactg
     1741 taacagctgt tgaaaaaccg gaatgcactc ttcattgcaa acatttcagg gatgaagtga
     1801 agatgaattg agtcgtagta tccac
//
LOCUS       DAZGVQ010000333         1822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000333 DAZGVQ010000000
VERSION     DAZGVQ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.336"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..303)
                     /locus_tag="ACHLAG_005280"
     CDS             complement(<1..303)
                     /locus_tag="ACHLAG_005280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658331.1"
                     /translation="MVVDRRCYSDGGLGFSPDGFRLRSRRCRNRGGSVVLENGEQKDA
                     RDFEAAKEQCRIDNQLEIACNVLWIVRLPLALYGHHFFRYLSNSITGAGIFFFALFF"
     gene            1056..1589
                     /locus_tag="ACHLAG_005281"
     CDS             1056..1589
                     /locus_tag="ACHLAG_005281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HFP0658332.1"
                     /translation="MLEQQFEDFEQQKRRDHQAQDQSKSAKQKRDRLNPPDWSQVTKA
                     GWNMLDSISEKVPRAFRLYSFFAQNIDKSCGAVVCDQKFLADYFGVSLRTIQYWIGDL
                     ESIGAIVKIPVGIYYAYALDPYQVWRGYNKSKKYAAFNAKTLTQKDGVIDRKLRLMLS
                     RREEFEGSLKTSNHDLD"
     gene            1602..>1822
                     /locus_tag="ACHLAG_005282"
     CDS             1602..>1822
                     /locus_tag="ACHLAG_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658333.1"
                     /translation="MRIKYESRWKLEELKLEICRSIDSLIDEYHIEYAMGINLYMNCE
                     DKEFNPVDIVNEDGKNIMFVVSNNEKIQKP"
BASE COUNT          556 a          375 c          436 g          455 t
ORIGIN      
        1 aaaaaaaagt gcgaagaaaa aaattcctgc acctgtaatt gagttagata aatatcggaa
       61 aaagtgatgg ccatacagag ccagcggcag gcggacaatc cacagaacat tgcatgcaat
      121 ttctagttga ttgtctatcc tgcattgctc ttttgccgcc tcaaaatccc ttgcgtcttt
      181 ttgctccccg ttttccagaa cgaccgagcc gccacggttc cggcagcgcc ttgagcgcaa
      241 ccggaaaccg tctggggaga accccagacc cccatcgctg taacatcgac ggtctacgac
      301 cacctaccaa ccgcgtggct gcctattccg tgcaaatggg ctttgcaggg ggctgacgcg
      361 gttcctggcg acctgaaatc ccctggttag gggtatcggc gcgcctgacc tcctaaaacc
      421 ctctgtaagc gtttttcgcg ccttccatgc cctgatgggc atttaaaacc cgtttcgtgc
      481 gtctgagagc ttctgagagc gtttgaagag ctatctggcg actcatcccc gaaagaacaa
      541 cagcggaatc accgataaca tgcgagaggt ggcaccgctc gccgcagccc cagcgaccga
      601 acgttagtga gcgagtgggc gaggaagcgg aagagaaccg gaaaccacat tgagcacgta
      661 cgcactgatg cggggcgttt tagcggtgaa cggactgcaa aaacatgatc gagctgttga
      721 acgtggagag cgcgaaatcg ggcaaaatca cggctgacga ggcttgcaga agagtaagcc
      781 agtatacact ccgctatcgc tacgtgactg ggtcagggct tcgccccgac accccaaatg
      841 ttcgatgaac taattaaatc ttgtgtagca aaaaagtgct ttgatagatc tttagagcaa
      901 aaatcgacat tctcttaaaa atcaaagcat tatgacacca acatacgaaa cgttatttgc
      961 atgtgtatac gcaaacatgt ttgcgtgtgt tgtttcgtaa tgtacgatat aaatttcgta
     1021 aggtacgaaa caacgttgca ggtaaggatg caagtatgct ggaacagcaa tttgaagatt
     1081 tcgaacagca aaaacggcga gatcatcagg cacaagatca atccaaaagt gctaagcaaa
     1141 aacgagatcg gttgaatcca cctgactggt cgcaggttac taaggcgggc tggaatatgt
     1201 tggactctat atctgagaag gttcccagag catttaggct atattctttt tttgctcaaa
     1261 atatagataa atcgtgtggg gctgttgtat gtgaccaaaa attcttagct gattatttcg
     1321 gtgttagttt aaggacgatc caatattgga ttggagattt agaaagtatt ggtgccatcg
     1381 tgaaaatacc agtaggtatc tattatgctt atgcgttaga tccgtatcag gtatggcgcg
     1441 gatataataa atctaaaaaa tatgcagcgt ttaacgcaaa aacattaact caaaaagatg
     1501 gcgttattga tcgcaagttg cggctcatgc tctctaggag agaagagttt gaaggctcgc
     1561 ttaagacatc taatcatgac ttggactagg tatgatgcgc tatgagaatt aaatatgaat
     1621 cacgttggaa attagaagaa ctaaaattag aaatatgtag atcaatagat tcgttaattg
     1681 atgaatatca tatcgaatat gcaatgggaa taaatctata tatgaattgt gaagataagg
     1741 agtttaatcc cgttgatatt gtcaatgaag atggaaagaa tattatgttt gtcgttagca
     1801 acaatgaaaa aatacaaaaa cc
//
LOCUS       DAZGVQ010000334         1804 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000334 DAZGVQ010000000
VERSION     DAZGVQ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.337"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            141..644
                     /gene="ftnB"
                     /locus_tag="ACHLAG_005283"
     CDS             141..644
                     /gene="ftnB"
                     /locus_tag="ACHLAG_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFP0658334.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(723..974)
                     /gene="yecJ"
                     /locus_tag="ACHLAG_005284"
     CDS             complement(723..974)
                     /gene="yecJ"
                     /locus_tag="ACHLAG_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFP0658335.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1089..1175)
                     /gene="azuC"
                     /locus_tag="ACHLAG_005285"
     CDS             complement(1089..1175)
                     /gene="azuC"
                     /locus_tag="ACHLAG_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFP0658336.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1438..1761
                     /gene="yecR"
                     /locus_tag="ACHLAG_005286"
     CDS             1438..1761
                     /gene="yecR"
                     /locus_tag="ACHLAG_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFP0658337.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
BASE COUNT          472 a          426 c          399 g          507 t
ORIGIN      
        1 tattcttatt tcagatcatc gtcagaattg actccacgat cacatttcgg accggcagaa
       61 aggaattatt ctgcaaacag taattatggt gttttgattt atcttgcacc tctccacttc
      121 tggatataag gatattaggt atggcaaccg ctggaatgct tctcaaactc aactctcaaa
      181 tgaaccgcga gttttacgca tccaatctct accttcacct gagtaactgg tgttctgaac
      241 agagtctgaa cggcaccgcc actttccttc gcgcccaggc acagagtaat gtgacccaaa
      301 tgatgcgcat gtttaacttt atgaagagtg tcggcgctac ccccatcgtt aaagccattg
      361 atgttcccgg tgaaaaactg aactctctgg aagaactgtt ccaaaaaacg atggaagaat
      421 acgagcaacg ttctagtacg ttggcacagt tagccgatga agcgaaagaa ctgaatgatg
      481 attcaaccgt caatttcctg cgcgatctgg aaaaagaaca gcagcatgat ggtctgttgc
      541 tgcaaaccat tcttgatgaa gtgcgcagtg cgaaacttgc gggtatgtgc cctgtgcaga
      601 ccgaccaaca tgttctgaat gtcgtgtcac accagctgca ttgatcatca tcggcgctaa
      661 tgcattgcgc cgatgaaggt tttgagaaac cgctgcctca tctgtttgaa gcagcggttt
      721 ttttaatggg attcaccctg tggggtaaac ttgagttcaa taagcgcgat ggctttttgg
      781 attgcccgca tggtgaccgg gtctgcggcg gcgggatggt tagtaaagtc gatattcttc
      841 agctgactgg acattttttc acgaacttca acgggcgcga ttacatcgag aacatccaga
      901 atttgtttga taaccaactg gcaagcaacc acatcagaaa ccagttcctg atcggcattc
      961 agcggctggg acatcgtaaa ctcctgatag cattttgaaa gccgttatag tagcggcttc
     1021 acatcttcag cgatagtcac atccaccgtc atcaggacac aaaaaaacct gccggagcag
     1081 gttttttgtt atcggaacat attgcctggc ggtacgtctt tgaacgtctt gcaatagtta
     1141 ttgaacatac ttttcaggat tttgcgcagt ttcatcgcgg cactccgacc atttgttata
     1201 caggtgttat tgtctttgcg cttataatat gacaaccatc acaaaaatca atctttatat
     1261 gatacaaatc acataaatac ccctttaatg ttataaaaat gataatcaaa aaacagcccc
     1321 cctatttctg acacctacag atggcaagaa atagcgcctg ccaggcgtct tttccggcca
     1381 ttgtcgcagc actgtaacgc gtaaaatagt gctttctctt actcttctgg ctggaccatg
     1441 agacttctga ttctgactct ttcattaata acgctggcgg ggtgtacggt tactcgccaa
     1501 gcccatgtga gcgaagttga cgcggcaaca ggtattgtac ggttagttta tgaccaggct
     1561 tttttgcagc atgcccatac cgatcgttat gtcagtcgcg ggatcgccga tcgcgcatgc
     1621 cagcaggaag gctataccca cgcggtcccc tttggtcagc cagtaggcaa ctgtagcctt
     1681 tttgcgggtt ccttatgttt gaatacggaa ttcactttgt catatcagtg ccatcattcc
     1741 gccttccctg tctttctcta aatattttaa tcagcgaggg gatcttcgct gattaaagaa
     1801 atag
//
LOCUS       DAZGVQ010000335         1755 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000335 DAZGVQ010000000
VERSION     DAZGVQ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.338"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            164..466
                     /locus_tag="ACHLAG_005287"
     CDS             164..466
                     /locus_tag="ACHLAG_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004142601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFP0658338.1"
                     /translation="MKIISVKTLRDFWAENPDAEQPLRAWVDEAAKADWKSPADIKVQ
                     YRTASILKNRRVVFNIKGNHYRLIVAIAYQRGWVFVKFIGNHKEYDAIDADTIELE"
     gene            469..828
                     /locus_tag="ACHLAG_005288"
     CDS             469..828
                     /locus_tag="ACHLAG_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007374288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0658339.1"
                     /translation="MNIKPIRTEQDYEAALRAVEPMFDNEPEIDTPEGDFFEVMCLLI
                     EEYEKKHYPIEPPSPIEAIRFRMEQQGLTVKDLEPAIGKKNRVYEVLNGTRSLTLPMI
                     RRLHNQFGIPLESLVGL"
     gene            complement(875..1267)
                     /locus_tag="ACHLAG_005289"
     CDS             complement(875..1267)
                     /locus_tag="ACHLAG_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0658340.1"
                     /translation="MIFVKRLQQVLQELNINQSELGRRLGVKPQSVQGWLKGVMPRMD
                     KLEKLAELSQHPVHWFFMEEETLGDKMAVSSNDNQPQLTEQQRKIISLLDELPQSDAE
                     QIIRDMEQKRDFYKRKLEELLRQKNKTA"
     gene            1394..1654
                     /locus_tag="ACHLAG_005290"
     CDS             1394..1654
                     /locus_tag="ACHLAG_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0658341.1"
                     /translation="MQKSTQVKILSIMSQSELGRRLGKTPQTISGWFKKRVPAEEVIP
                     ACEALDWGVTPHELRPDKYPNPTDGLPVEYQANAQAAAGVDS"
     gene            1651..>1755
                     /locus_tag="ACHLAG_005291"
     CDS             1651..>1755
                     /locus_tag="ACHLAG_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658342.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYF"
BASE COUNT          513 a          346 c          411 g          485 t
ORIGIN      
        1 tgaccactgc cgggtaactc gaagtattgc cctgcgttct gtggggcggg gtgggtttat
       61 ggatacaatt tacaaattaa aatttaactg gtcaacatgt ttattattaa atttgtaatt
      121 gcgagctgtt gcatcagtcc caaaatggga ctactataca gttatgaaga ttatctcagt
      181 taaaacactc agggattttt gggcggagaa tcctgatgca gaacaaccgc taagggcatg
      241 ggtggatgag gcggcaaaag ctgactggaa aagcccagca gacataaagg tacaataccg
      301 aacggccagt atattgaaaa accggcgcgt ggtattcaac ataaaaggta atcattaccg
      361 tttaattgtt gcaattgcgt atcagcgagg atgggtattt gttaaattca tcggaaacca
      421 caaggaatac gatgctattg atgctgatac catcgaactg gagtaagcat gaacatcaaa
      481 cctattcgta cagagcaaga ttatgaagcc gcgttgcgtg ctgttgaacc catgttcgac
      541 aatgagcccg aaattgacac gcctgagggg gatttttttg aggtgatgtg tttgctaata
      601 gaggaatatg aaaaaaaaca ttatcccatt gagccaccat ccccaattga agctataaga
      661 tttcgcatgg aacagcaggg gctgactgtg aaagatttgg aacccgcaat tgggaaaaaa
      721 aatcgggttt atgaggtgtt gaatggtacc agaagcctta cgttaccaat gattcgccgt
      781 cttcataatc aatttggtat ccccctggaa agcctggttg gattataaaa tctgctagtc
      841 atttgcctga tgctcgttcc agaaaaggaa tgcatcaggc agttttgttt ttctgccgca
      901 gtaactcttc aagtttccgt ttatagaaat cgcgtttttg ctccatatca cgaatgatct
      961 gctctgcgtc gctttgaggt aactcatcta aaagcgatat gatttttcgt tgttgttctg
     1021 taagttgcgg ttggttgtca ttactggata cagccatttt atcgccgaga gtttcttctt
     1081 ccataaagaa ccaatggacg ggatgttgtg agagctctgc taatttttcc agtttatcca
     1141 ttcttggcat cacgcctttc aaccaacctt gcacggattg gggttttaca ccaagacgtc
     1201 ttcccagctc tgactggttt atattcaatt cctgcaacac ctgctgaagg cgttttacaa
     1261 agatcatcac cacccctcgt aaactagttc cgcgatccta cagaaaaaat tgataagtgg
     1321 cattacaaat agaagttgaa atttaagttt aaatttgtaa ttatcggtgt catcgtaaag
     1381 ttcggaggga aacatgcaaa aaagtactca agtgaaaatc ctgtcaataa tgagccaatc
     1441 agaattaggg cgtcgtcttg gtaaaacacc gcaaaccata agtgggtggt ttaaaaaacg
     1501 agtgccagcg gaggaggtta taccagcatg tgaggcgctt gactggggag taactccgca
     1561 tgaattgcgc cctgataaat accctaatcc aaccgatggt ttacctgttg agtatcaggc
     1621 taacgcacaa gcagcggcgg gagttgattc atgaaaatca agcatgagca catccgcatg
     1681 gcgatgaatg cgtggctgct ttatccgagg gtagggcgca aaaaaatcgc tgatgatata
     1741 gcgacggcat atttt
//
LOCUS       DAZGVQ010000336         1749 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000336 DAZGVQ010000000
VERSION     DAZGVQ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.339"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1749
                     /locus_tag="ACHLAG_005292"
                     /pseudo
     CDS             1..1749
                     /locus_tag="ACHLAG_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family protein"
BASE COUNT          614 a          343 c          322 g          470 t
ORIGIN      
        1 cttgtggagt ttaccctcaa ggattaaaag acagagaaat tatatcaact tttcacgcag
       61 aaaaagttat taatgatctg ttaaaaaacg attataaaat ttccctttct ccagatacaa
      121 cttatcgaga gttgaataaa gcagcacagc gtagcattac agcgccagac aggataggag
      181 aaggaaaatc atgggtttat caacgagata caatgattga aagaggtgat aacagcggtg
      241 ttcatcagta tggtcctgct gaacatttca cccacattat atctgacaaa ccgtccccaa
      301 aagatgagta tattgcatat gctattaaca ttcctgacta tgagctggca gccgatgtat
      361 aaatattaac gtgacgtcac cttccgaaca gcaagaaaca tttaaaatac taatcaatcc
      421 agaacatcta cggcaaacac ttgagcgtaa atctcttact gctgttcaga aatcacaatg
      481 tgaaatcata acccccaaaa aacctggcga agcgattctt catgctttta atgccaccta
      541 ccagcaaatc agggaaaata tgtctgagtt tgcacgttgc cattatgggt atatacaaat
      601 ccctccagtg acaactttcc gcgccgacgg accagaaact cccgaagaag aaaagggtta
      661 ctggtttcat gcttatcaac ccgaagatct ttgtaccatc cataatccaa tgggagattt
      721 gcaggatttt attgcattgg ttaaagatgc taaaaaattt ggtatcgata tcattcctga
      781 ttataccttt aactttatgg gaattggggg tagtggtaaa aatgacctgg attatccctc
      841 tgctgatata cgagcgaaga tcagtaaaga tatagaaggt ggtatcccta gctattggca
      901 aggtcaggtt ttgattccat tcattaaaga tccagtaaca aaagaacgta aacaaatcca
      961 tccagaagat atacatctca ctgcaaaaga cttcgaagca agtaaagata acatctctaa
     1021 ggatgaatgg gaaaacctcc atgcattaaa agaaaagcgt ttaaatggaa tgcctaaaac
     1081 aacacccaaa agtgaccagg ttattatgtt gcaaaatcaa tacgttcgtg aaatgcgaaa
     1141 atatggcgta cgaggtttac gttatgatgc ggcaaaacac tcaaaacatg aacaaataga
     1201 aagatcaata accccaccgc ttaaaaatta taatgagcgg ttacacaata ctaacttatt
     1261 taacccaaaa tatcataaaa aagccgttat gaattacatg gaatatctgg taacttgtca
     1321 gttggatgaa caacaaatgt catcactgct ttatgaaaga gatgatttaa gcgccattga
     1381 tttttcattg ctcatgaaaa cgataaaagc cttttcattt ggtggagatc ttcaaaccct
     1441 tgcatcaaaa ccgggttcca caatctcaag tatcccatca gaaagacgga tattgattaa
     1501 cattaaccac gattttccta acaatggtaa tcttttcaat gactttctat ttaaccatca
     1561 acaagatgaa caattagcaa tggcatatat ggccgctctg ccgttcagca ggcctttagt
     1621 ttactgggat ggctaagtat taaaatcaac gactgaaatt aaaaattatg atgggtccac
     1681 gcgtgttggc ggtgaggcgt ggcttaataa aggttgctct acctatcagc agctctacaa
     1741 tgaattcca
//
LOCUS       DAZGVQ010000337         1740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000337 DAZGVQ010000000
VERSION     DAZGVQ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.340"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            79..357
                     /locus_tag="ACHLAG_005293"
     CDS             79..357
                     /locus_tag="ACHLAG_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658343.1"
                     /translation="MAHDTKLYNSDNSAVFASRHGRRSHAFKSDWFQHAPCTEEQAEW
                     LIQNYRRRGYEFRKTLSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            359..1408
                     /locus_tag="ACHLAG_005294"
     CDS             359..1408
                     /locus_tag="ACHLAG_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP0658344.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPIFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIRHAMNGTQERE
                     LSLAELSWWAVCNQVADALPEAALRRSLGLRAEKIRSVYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNSPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            1421..>1740
                     /locus_tag="ACHLAG_005295"
     CDS             1421..>1740
                     /locus_tag="ACHLAG_005295"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP0658345.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVRGQ
                     LVLGG"
BASE COUNT          410 a          456 c          465 g          409 t
ORIGIN      
        1 ttttttcctg gcattctggt ttacaattcg catgccagcc tgaacaactg gcacctgctg
       61 cgccagcaga gacaaccgat ggcgcacgat accaaattat acaattctga taattcagcc
      121 gtctttgcca gcaggcacgg gcggcgttct catgcattca aatctgactg gttccagcac
      181 gccccatgca ctgaagaaca ggccgaatgg ctgattcaga actaccgcag acgtgggtat
      241 gagtttagga aaaccctcag cctcgattat cgtcactgga taatctccgt caggcttcct
      301 tactctgaac gcccaccgcg tccgtcccgc acattccagc aacgcatctg gaggtaacgt
      361 gcgggtatta cttcgacctg tactggttcc ggaactcggg ctggtggtcc ttaagccagg
      421 ccgtgaatcc atgcctatat tccacaatac ccgggtactg gtggagccgg aaccgaaaag
      481 catgcgtaat ctgccgtccg gggtcgttcc tgccgttcgc cagccgctgg tggaagacaa
      541 aacattgctg ccgtttttca gtaacgcacg ggtaattcgt gctgctggtg gtgctggtgc
      601 attgtctgac tggctgttgc gccatattaa atcctgccag tggccacacg gcgattatca
      661 tcacagcgaa accgttattc accgttatgg taccggcgca atggtgttgt gctggcactg
      721 cgacaaccag ctgcgtgacc agacatccga atcactcgag caacttgctc atcaaaactt
      781 gtcagcatgg atgattgacg tcatacgcca tgcaatgaat ggcacgcagg agcgggaatt
      841 atcgctggct gaattatcct ggtgggcggt ctgcaatcag gtggcggacg cgctaccaga
      901 ggcagcatta cgtcgttctc tggggttacg tgcggaaaaa attcgctcag tataccgcga
      961 gagcgacatc gtgccgggag agcagaccgc caccagcatc ctgaaacagc gcacaaaaaa
     1021 tcttgcgccg ctgcctcacg cccaccagca acagaactca ccacaggaaa agacggtggt
     1081 cagcattgcc gttgatcctg agtctccgga atctttcatg aaacgaccta aacgtcgccg
     1141 ctgggttaac gagaaataca aacgctgggt gaagacacag ccgtgtgcgt gttgtggtaa
     1201 gccagccgac gatccacatc acctgattgg tcatggtcag ggcggaatgg ggacaaaatc
     1261 tcacgatatt ttcacgctac cgctgtgtcg ggagcatcac aacgagcttc atgcggatcc
     1321 gctggcgttc gaagaaaagc atggttctca ggttgattta atttttcgtt ttcttgatca
     1381 cgcctttgca actggcgtgc ttgggtaaaa gaggtgactg atgctcatag atttggtttt
     1441 accttacccg ccgacggtga acacttactg gcgacgccgt ggcagcacat attttgtatc
     1501 aaaagccggg gagcgttatc gccgggcagt ggcgcttatt gttcgccagc agcgcttgaa
     1561 attaagcctg tccggacggt tggcaataaa aattattgca gaaccaccgg ataagcgccg
     1621 ccgtgacctg gacaatattc tgaaagcacc gctggatgcg cttacgcatg cggggttgtt
     1681 aatggacgat gagcagtttg atgaaatcaa tattgtacgt ggtcagcttg ttcttggtgg
//
LOCUS       DAZGVQ010000338         1705 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000338 DAZGVQ010000000
VERSION     DAZGVQ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.341"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..312
                     /locus_tag="ACHLAG_005296"
     CDS             <1..312
                     /locus_tag="ACHLAG_005296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0042262 - DNA protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP0658346.1"
                     /translation="DWCKTNRNELTNGGETKTANLTTGTVSWRLGNPSCSVSRDVEGV
                     IEMLRRMGLERFIRTKEEVNKQAVLAEPDAVKGIAGIKVNKGAENFHVEPFEQDAGLN
                     K"
     gene            397..975
                     /locus_tag="ACHLAG_005297"
     CDS             397..975
                     /locus_tag="ACHLAG_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5420 family protein"
                     /protein_id="HFP0658347.1"
                     /translation="MSIKYRYFKMNESDSRHYHREWLDCVGRERQKLIDDFLSNQNAR
                     GYCYSWYCGDFFVKSILVHEDVDVGRNKRVLSDKFDEDGRTLFSVKPDRRFREGKKLN
                     KALNILNEKLKQLPSFSTWMVNKLDCYFEVFGVSNGRTVMACSSAGFYGDKGAVVVRI
                     PVGESDNAFSPEKLHPSLMAIKHSEFIAITEE"
     gene            979..1512
                     /locus_tag="ACHLAG_005298"
     CDS             979..1512
                     /locus_tag="ACHLAG_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658348.1"
                     /translation="MIDAKVLEGVKNWLSIYGRLTCGILAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQEGAADDVQWCSFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITEHVLRY
                     LIVDRTAAPAAISAEVE"
     gene            1512..>1705
                     /locus_tag="ACHLAG_005299"
     CDS             1512..>1705
                     /locus_tag="ACHLAG_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658349.1"
                     /translation="MFFKTSNPAALAAWQKYQQDCQTVKDEAKRLEAVLNVACRSVFE
                     FSISGFCFKGLRFMDDKYPF"
BASE COUNT          431 a          334 c          481 g          459 t
ORIGIN      
        1 gactggtgta aaaccaaccg caatgagctg acgaacggcg gtgaaaccaa aactgccaat
       61 ctgaccaccg gaacggtgtc atggcggctg ggaaatccat catgcagcgt cagtcgtgat
      121 gtggaaggtg tgattgaaat gctgcgtcgt atggggcttg agcgcttcat ccgcacgaaa
      181 gaggaagtga ataagcaagc tgtcctggca gagccggatg cggtgaaagg gattgcgggt
      241 attaaggtga ataaaggcgc tgaaaatttt catgtcgagc cttttgaaca ggacgccgga
      301 ctgaataaat aacaccgcat taaatcttta aatatcacat cgttttaatt atggcgctcg
      361 cgtcagggga ctgctcgcgc ctgaatgagg catgtcatga gtattaaata tcgttatttt
      421 aaaatgaatg agagcgattc ccgtcattat cacagggaat ggcttgattg tgttggcagg
      481 gagcgccaga aattgattga tgattttctc agtaatcaga atgccagagg gtattgttat
      541 tcctggtatt gtggggattt ttttgtcaaa tcaatactgg tgcatgagga tgttgatgtc
      601 ggaagaaata aacgggtgtt gtctgataaa tttgatgaag atggccgtac tttattttca
      661 gttaagcccg accgcagatt cagagaggga aaaaaactga acaaagcact gaatatctta
      721 aacgagaagt taaaacagct tccgtcattc agtacctgga tggttaataa actggactgc
      781 tattttgagg tttttggtgt cagcaacggc cgcacagtta tggcgtgctc ttctgccggg
      841 ttctatggcg acaaaggggc tgttgtggtt cgtattcctg tcggtgaatc agataatgcc
      901 ttttcacctg aaaagctgca tccgtcattg atggcaatca agcattccga attcatcgca
      961 ataacggagg aataattcat gattgatgca aaagtgcttg aaggggttaa aaactggctg
     1021 agtatttacg ggcgtctgac ctgcggcatt ctggctgaaa aaatgaatat gccgccatcc
     1081 tcgatggttt attttctgcg tgatgcggtc gatgccgggg tgctgacgga atgtaacggt
     1141 ttttatgata ttccgcgtcc ccgcccggtg cagccggttc gtcgcaaatg cagccaggaa
     1201 ggtgcggctg atgatgttca gtggtgcagc ttcagaaaat ccctgccgtg gattgagggg
     1261 catgatattc cgtcgatggc gtgggaattt gctcagggcg ttctgacctg tgaaaccgtt
     1321 tatgtggtgg ctgaagttga tgagcaggcc atgaaagaag gcgtgcccca gtttgtgatg
     1381 gcgtatatcg acattcgcct gggcgtcatt atctgcggtt taagcggctg gaatatcacc
     1441 gaacatgttc tgcgttacct gattgttgac cggacggctg cgcctgccgc gatttctgcg
     1501 gaggtagagt aatgttcttt aaaacatcaa accctgccgc gctggctgcg tggcaaaaat
     1561 accagcagga ctgccagacg gtaaaagatg aggcgaaacg ccttgaggcc gtgctgaatg
     1621 ttgcgtgccg gtcggtattt gaattcagta tcagtggctt ttgttttaag gggctgcgct
     1681 ttatggatga caaatatcct tttca
//
LOCUS       DAZGVQ010000339         1691 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000339 DAZGVQ010000000
VERSION     DAZGVQ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.342"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..99
                     /locus_tag="ACHLAG_005300"
     CDS             <1..99
                     /locus_tag="ACHLAG_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFP0658350.1"
                     /translation="LVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            168..389
                     /locus_tag="ACHLAG_005301"
     CDS             168..389
                     /locus_tag="ACHLAG_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP0658351.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            552..920
                     /locus_tag="ACHLAG_005302"
     CDS             552..920
                     /locus_tag="ACHLAG_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP0658352.1"
                     /translation="MSDTHHETNYPDDYNNRPWWGLPCTITPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELILTSGELNPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYMAVYPTPETKK"
     gene            1010..1384
                     /gene="cbtA"
                     /locus_tag="ACHLAG_005303"
     CDS             1010..1384
                     /gene="cbtA"
                     /locus_tag="ACHLAG_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
                     /protein_id="HFP0658353.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQTLLSRLLDQHYGLTLNDTPFS
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPRFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAK"
     gene            1381..>1691
                     /locus_tag="ACHLAG_005304"
     CDS             1381..>1691
                     /locus_tag="ACHLAG_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312567.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP0658354.1"
                     /translation="MMKLALTLEADSVNVRALNMGRIVVDIDGVNLAELINNVAENGY
                     SLRVVEEFDQQSTCTPPPFTALAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHF
                     TG"
BASE COUNT          388 a          485 c          413 g          405 t
ORIGIN      
        1 ctggtacagg cactgggcct ggtggatatc cgggtgccgg accatctgat agtcggtggc
       61 agccaggttt tctcctttgc ggaacacggt ctgctttaac ccgtcaccgc cacaatcaca
      121 tcacacctgt tttcactttt atcttccgtc ttcagaggta tcctattatg aaaattatca
      181 cccgtggtga agccatgcgt attcaccaac aacatccgac atcccgtctt tttccgttct
      241 gtaccggtaa ataccgctgg cacggcagtg ctgaagcgta taccggtcgt gaagtgcagg
      301 atattcccgg tgtgctggcc gtgtttgctg aacgccgtaa ggacagtttt ggcccgtatg
      361 tccggctgat gagcgtcact ctgaactaat gttccgtcac ccggaaaacg agtacgacca
      421 gatgacctca gacgccgctg tggatgaggc ccttctcatc aatgaataca tcttcaccga
      481 agccggtctc cacaccggct gacctgtcat cgttaacccc ttcttaaccg attttctgtg
      541 aggactttat cgtgtcagac acgcaccatg agacaaatta tcccgacgac tataacaacc
      601 gcccctggtg ggggctgccc tgcaccataa caccctgttt tggcgcccgt ctggtgcagg
      661 agggtaaccg gttgtattac cttgccgacc gcgccggtat cagaggccgg ttcagcgacg
      721 cggatgcgta ccatctggac caagcctttc cgctgctgat gaaacaactg gaactcatac
      781 tcaccagcgg tgaactgaat ccccgccatc agcataccgt cacgctgtat gccagaggac
      841 tgacctgcga agccgacacc ctcggctcct gtggttacgt ttatatggct gtttatccga
      901 cgcccgaaac gaaaaagtaa atcatcagaa taaccttctg ctccggcctg gtgttttcac
      961 cacgccactt tcccattttt catctctgca tatcaggaaa atcttcagta tgaaaacatt
     1021 acctgtatta cccgggcagg cggccagttc gcgtccgtct cctgttgaaa tctggcagac
     1081 actgctgtcc cgcctgctgg accagcatta cggccttacg ctgaatgaca caccgttctc
     1141 cgatgaacgt gtgattgagc agcatattga ggcaggcatt tcactgtgtg acgcagtgaa
     1201 ctttctcgtt gaaaaatacg cgctggtgcg taccgaccag ccccggttca gcacttgtcc
     1261 ccgctctcag ttaataaaca gtattgatat cctccgggct cgccgggcaa ccggcctgat
     1321 gacccgcgac aattacagaa cggtaaataa cattacccgg ggtaagcatc cggaggcgaa
     1381 atgatgaaac tggccctgac gctggaagct gacagcgtta acgtgcgggc gctgaacatg
     1441 gggcgcattg tcgttgacat tgatggtgtt aatctcgctg aactgattaa caatgtcgct
     1501 gaaaacggtt attcactccg cgtggtggag gaattcgacc aacagtcaac ctgcacacca
     1561 ccaccgttta cggcccttgc cggcatacgc tgcagtaccg cacatatcac ggaaaaggac
     1621 aacgcctggc tgtactcgct gtcgcatcag accagtgacg ttggtgaatc agaatggatt
     1681 catttcacag g
//
LOCUS       DAZGVQ010000340         1681 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000340 DAZGVQ010000000
VERSION     DAZGVQ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.344"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            130..345
                     /gene="essD"
                     /locus_tag="ACHLAG_005305"
     CDS             130..345
                     /gene="essD"
                     /locus_tag="ACHLAG_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFP0658355.1"
                     /translation="MKSMDKLTTGVAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            350..661
                     /locus_tag="ACHLAG_005306"
     CDS             350..661
                     /locus_tag="ACHLAG_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
                     /protein_id="HFP0658356.1"
                     /translation="MTQDYELVVKGVRNFENKVTVTVALQDKERFDGEIFGLDVAMDR
                     VEGAALEFYEAAARRSVRQVFLEVAEKLSEKVESYLQHQYSFKIENPANKHERPHHKY
                     I"
     gene            658..1191
                     /locus_tag="ACHLAG_005307"
     CDS             658..1191
                     /locus_tag="ACHLAG_005307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0658357.1"
                     /translation="MNTKIRYGLSAAVLALIGAGASAPQILDQFLDEKEGNHTMAYRD
                     GSGIWTICRGATVVDGKTVFPNMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            1188..>1681
                     /locus_tag="ACHLAG_005308"
     CDS             1188..>1681
                     /locus_tag="ACHLAG_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFP0658358.1"
                     /translation="MNQIFMVIFLVLSGFIVGNVWSDRGWQKKWAERDAAALSQEVNA
                     QFAARIIEQGRTIARDEAVKDAQQKSAEISARAAYLSDSVNQLRAEAKKYAIRLDAAK
                     HTADLAAAVRGKTTKTAEGMLTNMLGDIAAEAQLYAEIADERYIAGVTCQQIYESLRD
                     KKHQ"
BASE COUNT          476 a          346 c          437 g          422 t
ORIGIN      
        1 taattactca catttttaat gtgtatgttt ctttagcgcc gttgagaaca acgtgtgctg
       61 tcaaaactac cccgtagact ccgatctttt caaacatatt gcaccatccg tgtacatcgg
      121 ggtgaggata tgaaatcaat ggataagtta acaacaggtg ttgcctatgg cacatcggcg
      181 ggtaatgctg gtttctgggc attgcagtta ctcgataaag taactccgtc acagtgggct
      241 gcaatcggtg tgctgggtag cctggttttt ggcctgctga cgtatctgac aaatctttat
      301 ttcaagatta aagaagacag gcgtaaggct gcgagaggag agtaatccaa tgactcaaga
      361 ctatgaactg gttgtgaaag gagtccgtaa ttttgagaat aaagttacgg taactgtagc
      421 cttacaggac aaagaacgct ttgacggtga aatttttggc ctggatgtcg ccatggaccg
      481 tgttgaagga gctgcgctgg agttttatga ggcagcagcc agaaggagcg tccggcaagt
      541 cttcctggaa gtagcagaaa aattgtcaga aaaagttgag tcttatctgc agcatcagta
      601 ctcctttaag attgaaaatc ctgccaataa gcacgagcgt cctcatcata aatatatatg
      661 aacacaaaaa tcagatacgg cctgtcggct gccgttctgg cgctgattgg tgctggcgca
      721 tctgctcctc agatacttga ccagtttctg gacgaaaaag aaggtaacca cacaatggca
      781 taccgcgatg gttctggtat atggaccatc tgtcggggtg ccacagtggt ggatggaaaa
      841 accgtttttc ccaatatgaa actgtcgaag gaaaaatgcg accaggtcaa cgccattgag
      901 cgtgataagg cgctggcatg ggtggagcgc aatattaaag taccactgac cgaaccacaa
      961 aaagcgggta tcgcgtcatt ttgtccctat aacattggtc ccggtaagtg tttcccgtcg
     1021 acgttttata agcggctgaa tgctggtgat cgtaaaggtg catgcgaagc gattcgctgg
     1081 tggattaagg atggcggacg cgattgccgc attcgttcaa ataactgtta cggtcaggtt
     1141 attcgtcgtg accaggagag cgcattaacc tgctggggga tagaacagtg aatcagatat
     1201 tcatggtgat ttttctcgtg ttgtcaggat ttatcgtcgg aaatgtctgg agcgaccgag
     1261 gatggcaaaa aaaatgggcg gaacgtgatg ctgccgcatt atcacaagag gtaaatgctc
     1321 aatttgctgc tcgaataatt gaacaggggc gaactatagc ccgtgatgag gctgttaaag
     1381 atgcgcaaca gaaatctgct gaaatttctg ccagggctgc ttatctgtct gatagtgtta
     1441 accagttgcg tgccgaagca aaaaaatatg ccatacgcct tgacgcagcg aagcataccg
     1501 cagatcttgc cgctgccgtc agaggcaaaa caaccaaaac cgccgaagga atgctcacca
     1561 acatgctcgg agatattgca gcagaagctc agctttatgc tgaaattgct gacgaacgct
     1621 acatcgcagg agtgacttgt caacagatct atgaatcttt aagagataaa aagcatcaaa
     1681 t
//
LOCUS       DAZGVQ010000341         1647 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000341 DAZGVQ010000000
VERSION     DAZGVQ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1647
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.345"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..1647
                     /locus_tag="ACHLAG_005309"
                     /pseudo
     CDS             3..1647
                     /locus_tag="ACHLAG_005309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
BASE COUNT          515 a          367 c          287 g          478 t
ORIGIN      
        1 ttatcagaac tcattataag tctcctcata gattgcacat cgatagcgac ataccaacac
       61 cttcatcaga gcctattaat aaatttgcgc gccagctcat caccctactt gatacctctg
      121 acttaagttc gatgctgtca tactgtgtta ttcaggaatt taccgcaaac tgtcgaaaaa
      181 tatcacaaaa ttgttattcc actgcccttt ttaccattaa ctttgccact tcacccatcc
      241 atgcagaaaa tatactcatt acattacact ataaaaaaga tttcatttcc ttattactgg
      301 aaaccacgcc tattaaagct aaccatttgc gaagcatact ggattatatt gaacaggaac
      361 agttaactgc tgaagatcgt aaccattgta tgaaactgtc taaaaaaatc catagagaaa
      421 aaactataca cccaacagta aatctcaatg gtagtgcatg ttttttgcaa tctccttctg
      481 acgctatttt ttgtcgccat ctgtcattgc aatacgccct tgattcattg agaaatggaa
      541 aaggcaaagt caacctgatt aaacattact cctccgttga atccatacag cagcatatcc
      601 ccttagtccg ggatgcggag ttcagagcat tacttcgcca tcctcctgca gggagtcgcg
      661 ttatcgcgag taaggatttt ggcttcgctt tagatatttt cttttgtcga atgatggcaa
      721 acaatgtcag tcatatgtcc gcgattttat atatagacaa tcatactttg tcagtaaggc
      781 tacgaataaa gcagtcagtg tatggacaat taaattatgt tgtgtctgtt tacgacccga
      841 acgataccaa cgttgccgtc agaggcaccc acaggacagc acggcgcttt ctctcgcttg
      901 ataagttcat cagttcagct cccgatgctc agacctgggc tgataggtat gttcgcaact
      961 gtgcaattgc tattcttcct ctattacctg agggagttcc agttgctatt ttagcgggta
     1021 tcacgacacg aaagccattt gcccctatac atccatcggc aatgttgtta ataatggcca
     1081 caggccagac tcaatagctt attacactat tcaaacagtt gcccatactc cctgaaaaag
     1141 aaatcattga aataataact gcgcagaata gcgttggtac acctgcttta tttctggcta
     1201 tgatgaacgg acaaactgac aacgtaaaaa tatttatgca agaaatccag tcactggtat
     1261 acaatcatat cattcatgaa gataatctgg ttaaattact gcaaactaaa agtgctaacg
     1321 aaacacctgg actctatatc tccatgttgt atggattcga tgaaataatc gatatctttc
     1381 tgaatgcatt aaccactcca atagcacaag aacttttaaa caaaaaaaat ggtgatggat
     1441 attttagcca tgaaaacacg tgatggtgaa ccagggttat ttgctgcaat ggaaaataat
     1501 caccctttgt gtgtcacacg gttcctctct aaagtttatg ggatcgccgt taaatataaa
     1561 ctcagcaaaa tcaacatcat ggacttatta aaaggcgcga cagcacatgg aacccctgct
     1621 ttatacatcg ccatgagcaa gggtaat
//
LOCUS       DAZGVQ010000342         1641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000342 DAZGVQ010000000
VERSION     DAZGVQ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.346"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..261
                     /locus_tag="ACHLAG_005310"
     CDS             <1..261
                     /locus_tag="ACHLAG_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658359.1"
                     /translation="AETQSALAAIRREDQERAARGEERRETMRKNLQADECARTSPPA
                     DVADSLQQHASAAENRRHLRERAGKSDQTDTGTGAAATHDMG"
     gene            248..379
                     /locus_tag="ACHLAG_005311"
     CDS             248..379
                     /locus_tag="ACHLAG_005311"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658360.1"
                     /translation="MIWADVADWGDRLVDALDSCNADKAAIAGLERQRTARMNTHEK"
     gene            390..>1641
                     /locus_tag="ACHLAG_005312"
     CDS             390..>1641
                     /locus_tag="ACHLAG_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP0658361.1"
                     /translation="MVPKHYQLVTAMTNKELAEKLAPMLREGWGLLGGPVSASEPGAP
                     FYLMQALVTDRDLPSAIPVPEGEENPVPGTTDGYDTGHTTEPEFYYVVPLAGQSNGMA
                     YGEGLPLPDTFDSPDPRIKQLARRSTVTPGGAACTYNDIIPADHCLHDVQDMSSLNHP
                     NADLSKGQYGCVGQGLHIAKKLLAFIPKNAGILLVPCCRGGSALTSGAEGAFSESSGA
                     TAESSLWGVGKPLYNDLLTRTKAALDKNPKNVLLSVVWMLGEFDLKSATYAQHPQLFL
                     NMVNQFRADLSAYAAQCTGGNASLVPWICGDTTYIWKQQYTSQYPVVYGAYQNKEAQK
                     IYFVPLMTDEKGANTPTNLPAEDPDLAEAGYYGAASRSNGNWTSSDRATHFSSWARRG
                     IISDRLATAILMHAGKTVAFLKGAP"
BASE COUNT          422 a          444 c          446 g          329 t
ORIGIN      
        1 gcggagacac agtcagcgct ggccgctatc cggcgcgaag atcaggagcg tgcagccaga
       61 ggggaagaac gacgtgaaac gatgcggaaa aacttacagg cggatgagtg cgcacgcact
      121 tctccccctg ctgatgttgc tgacagcctg caacaacacg ccagcgccgc tgaaaaccgg
      181 cgtcatttgc gggagcgtgc cggaaagtct gaccagacag acaccggtac cggtgctgcc
      241 gcaacacatg atatgggctg atgtggctga ctggggagac cgactggtag atgccctgga
      301 cagttgtaat gccgataaag ccgccattgc cgggctggag cggcagcgga cagcacgaat
      361 gaacactcat gaaaaataac ggagtcacta tggtaccgaa acattatcag cttgtgacgg
      421 caatgacgaa caaggaactt gccgaaaaac ttgcgcccat gctcagggaa ggatggggac
      481 ttcttggggg acctgtctca gcatcagagc cgggagcacc attttacctg atgcaggcac
      541 tggtcactga cagagacctg ccatcagcca ttccggtacc agaaggcgag gaaaaccccg
      601 taccgggcac taccgacgga tacgacactg gacacacaac tgagccagag ttttattatg
      661 tggtgccact ggctggtcag tccaatggca tggcatacgg tgaggggctg ccattacccg
      721 atacattcga cagtcctgat ccacgaatta aacagttagc gcgtcgcagt acggtaacac
      781 cgggcggcgc tgcatgtacc tataacgata ttattccggc agatcactgt ctccatgatg
      841 tacaggatat gagcagcctt aatcatccga acgccgatct gtcaaaaggt cagtatggct
      901 gcgtcggaca gggtttacat attgccaaaa aattactggc gtttatcccg aaaaatgcag
      961 ggatcctcct ggtaccttgc tgtcgtggag ggtcagcgct tacttccggc gctgaggggg
     1021 cattcagtga atcttcagga gcaacggccg aatcatccct gtggggcgtc ggcaaaccgc
     1081 tgtataacga tctgcttacc cgtacaaaag ccgcgctgga taaaaacccc aaaaacgtcc
     1141 tgttatccgt ggtctggatg ctgggagagt ttgatctgaa atcagcaaca tacgcacagc
     1201 acccgcaatt attcctgaac atggtgaatc agttccgcgc cgatctcagc gcatatgctg
     1261 cacagtgcac cggaggcaac gcatcacttg ttccctggat ttgcggcgat accacctata
     1321 tctggaaaca acaatatacc tctcagtacc cggtggttta cggtgcatat cagaacaaag
     1381 aagcgcaaaa aatttatttt gtgcccctga tgaccgatga gaaaggtgct aacacgccaa
     1441 caaacctgcc tgccgaagat cctgacctgg cagaagccgg ttattacggt gctgcatccc
     1501 gtagtaacgg aaactggacc tcttcagatc gcgccacgca tttcagttca tgggcccgcc
     1561 gcggcattat ttctgaccgc ctggcaacgg cgatcctgat gcatgccggg aagaccgtcg
     1621 ccttcctgaa aggtgcaccc g
//
LOCUS       DAZGVQ010000343         1610 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000343 DAZGVQ010000000
VERSION     DAZGVQ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.347"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1150
                     /locus_tag="ACHLAG_005313"
     CDS             <1..1150
                     /locus_tag="ACHLAG_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP0658362.1"
                     /translation="LSRKPQVTWYGWDGDRLTTIQNDRSRIQTIYQPGSFTPLIRVET
                     ATGELARTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRWL
                     ASCGLTVEQMQNQMDPVYTPARKIHLYHCDHRGLPLALISTEGATAWCAEYDEWGNLL
                     NEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYP
                     LNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPDIAKQLRLPYPIDQASSA
                     PNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQYPGMDTKTASDYLPQTNWPTT
                     QLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQRTSRGIRK"
     gene            1147..>1610
                     /gene="ybfC"
                     /locus_tag="ACHLAG_005314"
     CDS             1147..>1610
                     /gene="ybfC"
                     /locus_tag="ACHLAG_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFP0658363.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKD"
BASE COUNT          468 a          361 c          421 g          360 t
ORIGIN      
        1 gctgtcacgg aaaccgcaag tgacctggta cggctgggac ggcgaccggc tgaccacgat
       61 acagaacgac aggagccgca tccagacgat ttatcagccg gggagcttca caccactcat
      121 cagagttgaa actgccaccg gtgaactggc cagaacgcag cgccgcagcc tggcggatgc
      181 gcttcagcag tccggcggcg aagacggtgg cagtgtggtg ttcccgccgg tgctggtgca
      241 gatgctcgac cggctggaaa gtgaaatcct ggctgaccgg gtgagtgagg aaagccgccg
      301 ctggctggca tcgtgcggcc tgaccgtgga gcagatgcaa aaccagatgg acccggtgta
      361 cacgccggcg cgaaaaatcc acctgtacca ctgcgaccat cgcggcctgc cgctggcgct
      421 catcagcacg gaaggggcaa cagcgtggtg cgcagaatat gatgaatggg gcaacctgct
      481 gaatgaagag aacccgcatc agctgcagca gcttatccgc ctgccggggc agcagtatga
      541 tgaggagtcc ggcctgtatt acaaccgcca ccgctattat gacccgctgc aggggcggta
      601 tatcactcag gatccgattg ggctgaaggg gggatggaat ttttatcagt atccgctgaa
      661 tccggttcag tatatagatt caatgggact ggcatcaaaa tatggacact taaataatgg
      721 cggatatgga gcgagaccca acaaaccgcc tacgcccgat ccaagtaaat tgccggacat
      781 agcgaaacaa ttaagactgc catatcctat tgaccaggcc agtagtgcgc ctaatgtttt
      841 caaaacattc ttcagagcat taagccctta cgactacaca ctgtattgca ggaagtgggt
      901 aaaaccaaat ctgacttgta cgccacagga tgattcccag tatccaggga tggatacaaa
      961 gacagcaagt gattacctgc cacagacaaa ttggccaaca actcaattac caccaggata
     1021 tacttgtgca gaaccctatt tattcccaga cattaataaa cccgatgggc cagcaacagc
     1081 agggatagat gatttgggtg aaattttagc taagatgaaa cagagaacat cgagaggaat
     1141 aagaaaatga aaagagtttt gttctttttg ctgatgatat ttgttagttt tggtgttata
     1201 gctgattgcg aaatacaagc taaagatcat gattgtttta ctattttcgc taagggtact
     1261 attttttccg cgtttcctgt cttaaataat aaagccatgt ggagatggta tcagaatgag
     1321 gacatcggtg agtattattg gcaaacagaa ctgggtacat gtaaaaataa taaattcaca
     1381 ccaagcgggg cgagactctt aattcgcgtc gggtcactac gccttaatga aaatcatgcc
     1441 atcaaaggaa cgttgcagga gttgataaat acagcagaaa aaacggcgtt tctcggcgat
     1501 cggtttagaa gttatataag agcgggtatc tatcaaaaaa agagcagtga tccagttcag
     1561 ttgttggcag tccttgataa ttcaatcatg gtaaaatatt ttaaagatga
//
LOCUS       DAZGVQ010000344         1606 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000344 DAZGVQ010000000
VERSION     DAZGVQ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.348"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..99
                     /locus_tag="ACHLAG_005315"
     CDS             <1..99
                     /locus_tag="ACHLAG_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFP0658364.1"
                     /translation="LVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            168..389
                     /locus_tag="ACHLAG_005316"
     CDS             168..389
                     /locus_tag="ACHLAG_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP0658365.1"
                     /translation="MKIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSTEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            468..836
                     /gene="cbeA"
                     /locus_tag="ACHLAG_005317"
     CDS             468..836
                     /gene="cbeA"
                     /locus_tag="ACHLAG_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFP0658366.1"
                     /translation="MSDTLPGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPEMKN"
     gene            925..1302
                     /gene="cbtA"
                     /locus_tag="ACHLAG_005318"
     CDS             925..1302
                     /gene="cbtA"
                     /locus_tag="ACHLAG_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
                     /protein_id="HFP0658367.1"
                     /translation="MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRNNYRMVNNITQGKYPEAKR"
     gene            1299..>1606
                     /locus_tag="ACHLAG_005319"
     CDS             1299..>1606
                     /locus_tag="ACHLAG_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP0658368.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDADGVELAELINVVCDNGYS
                     LRVVDESDRTSTDSIPPSAALSGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     G"
BASE COUNT          369 a          454 c          408 g          375 t
ORIGIN      
        1 ctggtacagg cactgggcct ggtggatatc cgggtgccgg accatctgat agtcggtggc
       61 agccaggttt tctcctttgc cgaacacggt ctgctttaac ccgtcaccgt cacaaccaca
      121 tcacacctgt tttcgctttt atcttctgtc ttcagaggta tcacactatg aaaatcatca
      181 cccgtggtga agccatgcgt attcaccaac aacatccggc atcccgtctt tttccgttct
      241 gtaccggcaa ataccgctgg cacggcagca ctgaagcgta taccggtcgt gaagtgcagg
      301 atattcccgg tgtgctggct gtatttgcag aacgccgtaa agacagtttt ggcccgtatg
      361 tccggctgat gagcgtcacc ctgaactgag ccaggaaggg cattctgatg agccgaatta
      421 tcgccaccat cgaaccattc ttaactgatt tctgtgagga ctttatcgtg tcagacacac
      481 tgcccgggac aacgcatccc gacgacaatc acgaccgccc ctggtggggg ctgccctgca
      541 ccgtgacgcc ctgtttcggg gcacgtctgg tgcaggaggg taaccagttg cattaccttg
      601 ccgaccgtgc cggtatcaga ggccggttca gcgacgcgga tgcataccac ctggaccagg
      661 cctttccgct gctgatgaaa caactggaac tcatgctcac cagcggtgaa ctgaatcccc
      721 gccatcagca taccgtcaca ctgtatgcga aagggctgac ctgcgaagcc gacaccctcg
      781 gctcctgtgg ttacgtttat ctggctgttt atccgacgcc cgaaatgaaa aattaactct
      841 ccagaatagc cttctgctac ggcctggtgt tttcaccacg ccacttttcc atttttatat
      901 ctgcatatca ggaaaatctt cagtatgaaa acattacctg tattacccgg gcaggcggcc
      961 agttctcgcc cgtctcctgt tgaaatctgg cagatactgc tgtcccgact gctggaccag
     1021 cactatggcc tcacgctgaa tgacacaccg ttcgctgatg aacgtgtgat tgagcagcat
     1081 attgaggcag ggatttcact gtgtgacgcg gtgaactttc tcgttgaaaa atacgcgctg
     1141 gtacgtaccg accagccggg attcagcgca ggagccccgt cgcagttaat caacagcatt
     1201 gatattctcc gggctcgcag ggcgaccggc ctgatgaccc ggaacaatta cagaatggta
     1261 aataacatta cccagggcaa gtatccggag gcaaaacgat gaaactggcc ctgacgctgg
     1321 aagctgacag cgttaacgta caggcactga acatggggcg aattgtcgtt gacgccgatg
     1381 gtgttgagct tgccgaactg attaacgtgg tctgcgataa tggctactcc cttcgtgttg
     1441 ttgatgaatc tgaccggacc tcaacagaca gcataccccc ctctgctgcc ctgagcggta
     1501 tacgctgcag taccgcgcat atcacggcaa aagacaacgc ctggctgtac tcgctgtcgc
     1561 atcagacgaa tgacaccggc gaatcagaat ggattcattt tacagg
//
LOCUS       DAZGVQ010000345         1582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000345 DAZGVQ010000000
VERSION     DAZGVQ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.349"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..215)
                     /locus_tag="ACHLAG_005320"
     CDS             complement(<1..215)
                     /locus_tag="ACHLAG_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP0658369.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKAEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGW"
     gene            complement(212..604)
                     /locus_tag="ACHLAG_005321"
     CDS             complement(212..604)
                     /locus_tag="ACHLAG_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP0658370.1"
                     /translation="MAKVFTPEEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSGCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQN
                     IICRECRQSEVMQRVLAFYRGNFQEVME"
     gene            complement(620..1390)
                     /locus_tag="ACHLAG_005322"
     CDS             complement(620..1390)
                     /locus_tag="ACHLAG_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP0658371.1"
                     /translation="METVFDALKAMGKATSVELAARLDISREEVLNELWELKRNGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            complement(1424..>1582)
                     /locus_tag="ACHLAG_005323"
     CDS             complement(1424..>1582)
                     /locus_tag="ACHLAG_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP0658372.1"
                     /translation="ERLLTKWAKNVGNGMSVPPVRRQLEGPKHPPGPTPIELLKQEYE
                     RRKAAGFV"
BASE COUNT          340 a          486 c          337 g          419 t
ORIGIN      
        1 tgccagccat tcagcatgta aatcgcatcc acacaacgaa tcattgccat gcaaatatcc
       61 atgtagtgcg gctgtgtcag cccgtccgga agtactgccg ggtttaagac ggtatgccct
      121 tcccgtttca gttcctcttc cgccttgtga aacgcctcac ggttgaaatt ttcatatccc
      181 gtcattggac cggcaatata aactctgacc ctcactccat cacctcctga aagtttcccc
      241 gatagaacgc cagcacacgc tgcataactt cgctctggcg gcactcacga caaattatgt
      301 tctgccgtct gttgtaacga cgtatttctc cgtcaggtaa ctttcgaatc agtgtcgggt
      361 cagcagcctt ctccggtgtc ttacgccata cgcgatacgc ctgctctgat ggaaataccc
      421 cgcaaccaga gagccagaca tcaccactgg ccgcaagcgc accagataaa cgacgaatag
      481 cggtcttact gacacccgtt ttatctgcca gttgtcgaaa agtttctcgt ccgctcaggc
      541 gcacgaattc cacaatgcgc gccttcactt cttcccgctc ttctggtgta aatacttttg
      601 ccataagcgc ctccggcaat cacttttccg atacaacacg gcgggaagaa tcagtaatct
      661 gtcgaacaat atcccggtgc ttgttcagct cccgcagcgc ggcgcagact cgctcccact
      721 tctgaacatc acttttcgcc ctgcgcagcg ccaggtttgc cctgcgaagg gacggaaaaa
      781 tcagctcatc tgcttgcgtt tcggtaaacg atggcaacgg ctgcacaatg tccgccacag
      841 tttctgtttt aatttcttcc tgtgttgcgg cttcccggac tggtaacgca gcacctgctg
      901 gctgaggaaa ggccttacca tcactttccg ttaccagcgc ggctttcggc tctgctggta
      961 aattatcgcc cggcatgcag taacgaaatt taccgttctg attaacgcgt gccagccgcc
     1021 ccgttgcggt taccaccgcc agcgtggagg caaccttgcg agtactgacg ccgaacttac
     1081 ccgccagttc ctcacacgtt ttagcaccat cctgaccgat aaactcaatc atcatgtctg
     1141 cggtaacttt ttgttcgacc tccccagtca gcatatcctg tgcttcagat tttactggcc
     1201 gctcttcggt tacccgggat tcaccttcgc cagccagaaa ccaggtgtga ccagttttat
     1261 caacgacgcc atttcttttg agttcccaca gctcgttgag aacctcttca cgactgatat
     1321 caagtcgcgc ggccagttct accgatgtgg cttttcccat tgctttcagt gcgtcaaata
     1381 cggtttccat taaaatttcc tccgacaaaa tcatttctca aattcagaca aaaccagccg
     1441 ctttccggcg ctcatattcc tgtttcagta actcaattgg cgttggccct ggcgggtgtt
     1501 ttggcccttc cagttgtcgt cgcactggcg gaacactcat gccgttacca acattctttg
     1561 cccatttggt caacagtcgt tc
//
LOCUS       DAZGVQ010000346         1565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000346 DAZGVQ010000000
VERSION     DAZGVQ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.350"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..178
                     /locus_tag="ACHLAG_005324"
     CDS             <1..178
                     /locus_tag="ACHLAG_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0658373.1"
                     /translation="KNALVRIPVAGKVCAYALDPHEVWKGYNTSKNYAAFVTKTLVNK
                     DGDIQRRIMAMFSN"
     gene            1264..>1565
                     /locus_tag="ACHLAG_005325"
     CDS             1264..>1565
                     /locus_tag="ACHLAG_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0658374.1"
                     /translation="MYIGTEEQRKKRLKELEKLNKESESDPKNNGPFTQVYQKGWERI
                     RELSKDKQGVVAIPLYSFLAEHIDPSCGAVVADQQFLADKLGVSRSTIKRWLNYLES"
BASE COUNT          407 a          383 c          412 g          363 t
ORIGIN      
        1 aaaaaacgcg ttagtcagaa ttcctgttgc tggtaaggtt tgtgcgtatg cactcgatcc
       61 acatgaagtc tggaagggat acaacacatc taaaaactac gcggcgtttg tcactaaaac
      121 actggtcaac aaagacggtg atattcagcg ccgaatcatg gccatgtttt caaattgagc
      181 cagcggcagg cacaggcaaa aaccccgttt ttggacaatc tgattcaaat gttaacattt
      241 gacatcgggt tgtctatcct gcattgctct tttgccgcct caaaatccct tgcgtgtttt
      301 tgctccccga tctccagaac gatcgagccg ccacggttcc ggcagcgcct tgagcgcaac
      361 cggaaaccgt ctggggagaa ccccagaccc ccatcgctgt aacaccgacg gtctacgacc
      421 acctaccaac cgagtggctg cctgtaccgt gcaaatgggc tttgcagggg gctggcgcgg
      481 ttcctggcga cctgaaagcc cctggctagg ggtatcggcg cgcctgacct cctgaaacgc
      541 tctgtaagcg tttttcgcgc cttccatgcc ctgacgggca tttaaacccc gtttcgtgcg
      601 tctgagagct tctgagcgcg tttgaagggc tatctgacga cttatccccg aaagaacaac
      661 agcggaatca ccgacaacat gcgagaggtg gcaccgctcg ccgcagcccc agcgaccgaa
      721 cgttagtgag cgagtgggcg aggaagcgga agagaaccgg aaaccacatt gagcacgtac
      781 gcactgatgc ggggtgtcgg ggcgaagccc tgaccagatg gcaaacggaa tatcgtcgcg
      841 ggtgacggta ttacatttgc acatcctgtc ccgatttctg aggcgtttta gcggtgaacg
      901 gacggcaaaa ccatgatgga gctgttgaac gttgagagcg tgaaatctgg caaaaatcac
      961 agctggtaag gcttgcagag gagtaagcca gtatacactc cgctatcgct acgtgactgg
     1021 gtcagggctt cgccccgaca ccccaaaaaa gtgttccgct ttgcgcaaca ctttcagcag
     1081 aaaagatatt taagggcgat tttgtaagtt attgattttt tagctacttg cattatgggt
     1141 tagctcacgc atgtgtggtc taaggttagc tcacgcatgt gtgagctagg tcgacctatc
     1201 atggtttacg tttgagctat gatagcttat atttgagcca tcattagacc gtggagattt
     1261 accatgtata tcgggactga agagcagcga aaaaagcgtc taaaagaact ggaaaaatta
     1321 aacaaagaat cggaatcaga tccgaaaaat aacggcccat tcacccaggt ttatcagaag
     1381 ggctgggaaa ggatccgaga actgtcgaag gacaagcagg gagttgtagc aataccgctt
     1441 tactcgtttt tagctgagca catagatccc tcatgcggtg ctgttgttgc cgatcagcag
     1501 tttttagcag ataaattggg tgttagccga agcacaatta agcgctggct caactactta
     1561 gaatc
//
LOCUS       DAZGVQ010000347         1542 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000347 DAZGVQ010000000
VERSION     DAZGVQ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.351"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..162
                     /gene="ybcN"
                     /locus_tag="ACHLAG_005326"
     CDS             <1..162
                     /gene="ybcN"
                     /locus_tag="ACHLAG_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFP0658375.1"
                     /translation="ITTIQSLRHTSDLDTGEMYVFLCKVEAWAVNIGCHLTIPQSCEF
                     QLLRDKQEA"
     gene            162..332
                     /gene="ninE"
                     /locus_tag="ACHLAG_005327"
     CDS             162..332
                     /gene="ninE"
                     /locus_tag="ACHLAG_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP0658376.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            325..615
                     /gene="ybcO"
                     /locus_tag="ACHLAG_005328"
     CDS             325..615
                     /gene="ybcO"
                     /locus_tag="ACHLAG_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFP0658377.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNSETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAAYAKECALEGMARTQVIWLKEGVIKA"
     gene            612..974
                     /gene="rusA"
                     /locus_tag="ACHLAG_005329"
     CDS             612..974
                     /gene="rusA"
                     /locus_tag="ACHLAG_005329"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFP0658378.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYRDNVARIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            971..1111
                     /gene="ylcG"
                     /locus_tag="ACHLAG_005330"
     CDS             971..1111
                     /gene="ylcG"
                     /locus_tag="ACHLAG_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFP0658379.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            1197..>1542
                     /gene="quuD"
                     /locus_tag="ACHLAG_005331"
     CDS             1197..>1542
                     /gene="quuD"
                     /locus_tag="ACHLAG_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFP0658380.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDI"
BASE COUNT          397 a          340 c          413 g          392 t
ORIGIN      
        1 atcaccacta tccagtcgtt acgccatacc tccgatcttg ataccggaga gatgtatgtc
       61 ttcctgtgta aggttgaagc ctgggcggtg aatattggct gccacctgac tattccgcag
      121 agctgcgagt tccagctgct ccgcgacaag caggaggcgt aatggctaca ccgcttattc
      181 gtgtcatgaa cggacacatc tacagagtat caaatcgtcg taagcgtaag cctgagctga
      241 agccatccga aataccaaca ctgctcggat ataccgctag cctggttgat aaaaaatggt
      301 tgcgactggc agcaaggagg aatcatggct gatttgagaa aagcagcgcg tggtcgggaa
      361 tgccaggtaa gaatccctgg cgtatgtaat ggcaattctg aaacgtctgt actggcacat
      421 atccggctgg ctggattgtg cggtaccggt atcaaaccgc cagacctgat tgccaccatt
      481 gcatgttctg cctgccacga cgaaatcgac cgccgcacac attttgtcga tgctgcatat
      541 gcaaaagaat gcgcgctgga aggtatggcg agaacacagg ttatctggct gaaagagggg
      601 gttattaagg cgtgaatacc tacagtatca cattaccctg gcctccgagc aataatcgct
      661 attaccgcca taatcgcggg cgcacgcacg tcagtgcaga ggggcaggca taccgcgata
      721 acgtcgcccg aatcattaaa aacgcaatgc tggatatcgg cctggctatg cctgtgaaaa
      781 tccgcattga gtgccacatg ccggatcgcc gtcgccgtga cctggataat ctgcaaaaag
      841 ccgcttttga cgcactcacc aaagcaggtt tctggctgga tgatgctcag gtcgttgatt
      901 accgcgttgt gaagatgcct gttaccaaag gtgggaggct ggaactgacc atcaccgaaa
      961 tggggaatga atgatgtttg agttttatat ggcagaactt cttcgccacc gctgggggca
     1021 tctgcgctta tatcgtttcc ccggttctgt tttgaccgat taccgaatac tgaagaatta
     1081 cgccaaaacc ctgacaggag caggagtatg aagtcagaga taacaatcaa ctaatactgt
     1141 tttgttgatt tttgcttgta attggcgttc tggtctgatt tttgtggagt aagttgatgc
     1201 gtgatattca gatggttctt gagcgttggg gagcgtgggc ggctaataat catgaagatg
     1261 tgacctggtc gtccattgcc gccggtttta agggattaat tacttcaaaa gtaaaatctc
     1321 gcccgcaatg ttgtgacgat gacgcgatga ttatttgcgg gtgcatggcc cgtctgaaaa
     1381 agaacaacag cgatttgcac gatttattag tagattatta tgtagtcggt atgacattca
     1441 tgtcactggc aggtaagcat tgctgctctg atggttatat cgggaaaagg ttacagaagg
     1501 ctgagggcat aattgaaggg atgttaatgg cattagatat cc
//
LOCUS       DAZGVQ010000348         1525 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000348 DAZGVQ010000000
VERSION     DAZGVQ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.352"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..159
                     /locus_tag="ACHLAG_005332"
     CDS             <1..159
                     /locus_tag="ACHLAG_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP0658381.1"
                     /translation="ERLLTKWAKNVGNGMSVPPVRRQLEGPKHPPGPTPIELLKQEYE
                     RRKAAGFV"
     gene            193..906
                     /locus_tag="ACHLAG_005333"
     CDS             193..906
                     /locus_tag="ACHLAG_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP0658382.1"
                     /translation="METVFDALKALKRASSQEVSARLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLTSEATEMATKTTAEMLINAIEQHGPQSADELALMFGITSRRANSTLAMAI
                     SKGRLIRVNQNGKFRYCLPGDNLPAEPKAASVTETAGKAFPQPAGVALPVREAETQEE
                     IKTESVAVTVQSQPSFTRKHPDSLILPSLHVANRELRRAKGQVQKWERVCAALRELNK
                     HRDIVRQIVDSSSRIVSEK"
     gene            922..1314
                     /locus_tag="ACHLAG_005334"
     CDS             922..1314
                     /locus_tag="ACHLAG_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP0658383.1"
                     /translation="MARVFTQEEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIR
                     RLSGALAASGDVWLSDCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRWQN
                     IICRECRQSEVMQRVLAFYRGNFQEVME"
     gene            1311..>1525
                     /locus_tag="ACHLAG_005335"
     CDS             1311..>1525
                     /locus_tag="ACHLAG_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP0658384.1"
                     /translation="MRVRVYIVGPMTGYENFNREAFHKAEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGW"
BASE COUNT          406 a          324 c          461 g          334 t
ORIGIN      
        1 gaacgactgt tgaccaaatg ggcaaagaat gttggtaacg gcatgagtgt tccgccagtg
       61 cgacgacaac tggaagggcc aaaacacccg ccagggccaa cgccaattga gttactgaaa
      121 caggaatatg agcgccggaa agcggctggt tttgtctgaa tttgagaaat gattttgtcg
      181 gaggaaattt taatggaaac cgtttttgac gcactgaaag cactgaaaag agcctcttca
      241 caggaggtgt cggcccgcct tggaatcagc cgcgaagatg ctgtcaacga gctgtggaag
      301 ctgaaacgcc gtggtgaagc tgataacaag gggtcgatgt ggtggctgac gagtgaggca
      361 actgaaatgg ccacaaaaac cactgcggag atgctgatta acgcaattga acagcatggt
      421 cctcagtcgg ctgacgaact ggcgttaatg ttcgggatta cctcccgccg ggcgaattca
      481 acgctggcaa tggcaataag caaaggtcgt ctgattcgag taaatcagaa cggtaaattt
      541 cgttactgcc tgccgggcga taatttacca gcagagccga aagctgcatc ggtaacggaa
      601 actgctggta aagcctttcc tcagccagca ggtgttgcgt taccagtccg ggaagcggaa
      661 acacaggaag aaattaaaac tgaaagtgtg gcggtcacag tgcagtcaca gccgtcgttc
      721 accagaaagc atccggatag tctgatttta ccatcgctgc atgtggctaa ccgcgaactg
      781 cgccgggcaa aaggtcaggt tcagaagtgg gagcgagtct gtgctgcgct gcgtgagctg
      841 aacaagcacc gggatattgt ccgacagatt gtcgattcct ccagtcgtat tgtgtcggaa
      901 aagtgattgc cggaggtact tatggcaaga gtatttacac aggaagagcg ggaagaagtg
      961 aaggcgcgca ttgtggaatt cgtgcgcctg agcggacgag aaacttttcg acaactggca
     1021 gataaaacgg gtgtcagtaa gaccgctatt cgtcgtttat ctggtgcgct tgcggccagt
     1081 ggtgatgtct ggctctctga ttgcggggta tttccatcag agcaggcgta tcgcgtatgg
     1141 cgtaagacac cggagaaggc tgctgacccg acactgattc gaaagttacc tgacggagaa
     1201 atacgtcgtt acaacagatg gcagaacata atttgtcgtg agtgccgcca gagcgaggtt
     1261 atgcagcgtg tgctggcgtt ctatcgggga aactttcagg aggtgatgga gtgagggtga
     1321 gagtttatat tgtcggtcca atgacgggat atgaaaattt caaccgtgag gcgtttcaca
     1381 aggcggaaga ggaactgaaa cgggaagggc ataccgtctt aaacccggca gtacttccgg
     1441 acgggctgac acagccgcac tacatggata tttgcatggc aatgattcgt tgtgtggatg
     1501 cgatttacat gctgaatggc tggca
//
LOCUS       DAZGVQ010000349         1515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000349 DAZGVQ010000000
VERSION     DAZGVQ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.353"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(527..>1515)
                     /locus_tag="ACHLAG_005336"
     CDS             complement(527..>1515)
                     /locus_tag="ACHLAG_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP0658385.1"
                     /translation="AMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMM
                     GARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVG
                     LMWMTFGWKVTLLYAIITAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGT
                     TYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGV
                     LIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGL
                     GALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT          464 a          313 c          314 g          424 t
ORIGIN      
        1 gaacaggaat ggattttctt ctacacacaa taaatataat gcgttatatc ttcctgatat
       61 aattaccttt aaattccctt tcatttccac ccggtaatat ttgtgtataa aaaaaaggca
      121 gtgatagtat cgtaaaggaa aacgttaaca actatcaaaa agcgctcatt ttaaaaagtt
      181 attatgttct ccgatagtgt gtgaagtaat atttttgtga tgttttagat gtgtaataat
      241 gaaccaggtt aaagtgtatt taaaaatgtg ttgtattagc gactgaaatc tgccttttaa
      301 aaaggaggag ggtactgaga caaagagtca ttgcttctcc gataaaatgt cagagaagca
      361 tgattacgtg gttattgtct gttgatgact tgttgaccga gccgtcatca ttctcaatgc
      421 attagttaaa tcatttgtga tttttaagga ttatcctgtc tatgattttc tacttagaag
      481 gatatggcac taactatgca tcttatagtt tactgctggc atctatttaa aatagcaatt
      541 gagtcaaata gcccatcaag atagccatac ctaatataac cgtcaggaat gcagctatca
      601 tcggcattct gaacattgat ttaagtaata tcacttccgt caggcttgcg ccggcactgc
      661 cgatgattaa tgccattaat gctcccagac ccattccttt tgtcatcaga acagatgcca
      721 gagggataac tgcctcagcc cggatataca gcggaatacc gacaacggcg cttaatggga
      781 tggcgaaggg attatctgct cctgcatgag cggcaatcca cgctgaggga ataaagccat
      841 aaataaaaga tcctattaat actcctaaca aaagatatgg cagtacatct ttaaactgtt
      901 gcaacgcatc tttggtggct agttttatgg gattaatact tactacagct ttggtactgc
      961 aacaggtaac tacggggttc tcaatggctt tagccgctgg actacaacaa ctcactttta
     1021 tgggcgtata tgtcgttccc ggcgaagttt ttgctggagc acaacaattt gctgatgagc
     1081 ttttactggc aatgatatga cgttcaaacc ccagagaatc caggataata ctggcaagaa
     1141 cggagacgcc ggcggtgata atcgcgtaca acaaggtgac tttccaacca aaggtcatcc
     1201 acattagtcc gacgataatg ggattaagta atggtgaaac aaataaaaaa gtgagagtcg
     1261 gaccaaaccc ggctttcgct gacaacaatc cacgtaacat gggtattgtc gagcaactac
     1321 agaacggggt aacggctccc aacagagcag ccaggagata gcctctccct tttctggccc
     1381 ccatcatctg ctggatttta tggtctggca ccttttgtct tatcaggctg acaccggcac
     1441 taatcactat aaacagcaac gaaagctcga cggcgagaaa cacaaacatc tctgcggcat
     1501 cttgcagcat cgcaa
//
LOCUS       DAZGVQ010000350         1508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000350 DAZGVQ010000000
VERSION     DAZGVQ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.354"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..657)
                     /locus_tag="ACHLAG_005337"
     CDS             complement(<1..657)
                     /locus_tag="ACHLAG_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFP0658386.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     N"
     gene            complement(821..916)
                     /gene="ymiC"
                     /locus_tag="ACHLAG_005338"
     CDS             complement(821..916)
                     /gene="ymiC"
                     /locus_tag="ACHLAG_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFP0658387.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(1030..1197)
                     /gene="yciX"
                     /locus_tag="ACHLAG_005339"
     CDS             complement(1030..1197)
                     /gene="yciX"
                     /locus_tag="ACHLAG_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFP0658388.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(1200..1340)
                     /locus_tag="ACHLAG_005340"
     CDS             complement(1200..1340)
                     /locus_tag="ACHLAG_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFP0658389.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT          408 a          361 c          347 g          392 t
ORIGIN      
        1 gttgatcata gtggtgtgcg agtcagtacc aacgagtgta tccggataag caatccattc
       61 accgtcctgc aattcactcc acactgcttt gccgagatat tcgaggttaa cctgatggca
      121 aatgcctgtg cctggcggca cgacgctaaa ccgactgaac gcttgctttc cccatttcag
      181 gaacacataa cgttcgtggt tgcgctccat ttccaggcgt acgttttctt caaatgcctc
      241 atcatcacca aaacgatcga cggtcaccga gtggtcaatg accaggtcga ccggtgagag
      301 cgggttaacc tttgcagtat cgccgccgag gcgtttaacc gcttcgcgca ttgccgccag
      361 atcaacaacg gcaggtacgc cggtaaagtc ctgcatcagc acccttgccg ggcggtaggc
      421 aatttcacgg tcagcatggg catttttcag ccatcctgcc agcgcgtgga tatcctcttc
      481 ggtaaccgag ttaccatcct gccagcgcag caggttttcg agcaaaactt tgagtgactt
      541 gggtagacgg gtgatatcgc ccagtgattt agcagcaagc ggcaggctgt agtagtggta
      601 agttttatct ttggcctgca acgtgtcctt actggcttct cgtagggttg acgacatagc
      661 tcctccttaa tgacagggtt gcgaagccct gattctcttc agggtgaata ttaaagataa
      721 cacaaacatc gcgtaacgat ttgataacaa cccaaattga taaaagagga gaaaccttgt
      781 ggcaaagcaa atgacaaccc cgccgcagcg gggtcaggga tcagtgagta atgatccaga
      841 ggagttcggc ccagaagagc atcgacagag aaaatgcgcc catccaggac caatatttca
      901 tatttgtgtt gatcataggc tacaccctta acggttatta ctttgtgttg agataactca
      961 acctgttatt gctgcggtgt attaaaagcc ctgtaattca ttaaaaatgg gtaataagaa
     1021 tgatgataat tattgtttcg gctgatcatc ttcagcaaac agttctataa aggcttgctg
     1081 ctgcgatgtt agttgccagt cgtctggaat aacgacggaa cattcccgtt tcgtttctgt
     1141 ttcattgtca ctatgctggc ataatggtga cgactccagt tgctcctgac cgaccatagt
     1201 tatccccaca ctttccaaat cagcagtgcg acaaccaccc agaaaagtgc agaaccaaga
     1261 aaaaccgcca gccaggcttt acgttttaat tcaggatcgc ggcggggttc ctgatttcca
     1321 gaaggcattg ctaacctcat ataatcgcca tcacttatca gcaagacgtt gaacgattgg
     1381 tgtaattaat tatacgatga gacaaatcct aaagaaactt tagtcaaaaa tccagcgaat
     1441 ttacgaatcg tctccagtga ataattcaat cttttgacct gaaatagata tttgaaaact
     1501 ggaatttg
//
LOCUS       DAZGVQ010000351         1506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000351 DAZGVQ010000000
VERSION     DAZGVQ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.355"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..369
                     /locus_tag="ACHLAG_005341"
     CDS             <1..369
                     /locus_tag="ACHLAG_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFP0658390.1"
                     /translation="RRMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEALIIDRVI
                     SEVLGEYPGLINILRQRYEGRGMSKLKMAERLNADHPEWTLVTCRRRIDQWLGISEFM
                     LHAPMRMAFVTEKKMLQTDQ"
     gene            586..783
                     /locus_tag="ACHLAG_005342"
     CDS             586..783
                     /locus_tag="ACHLAG_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP0658391.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            complement(966..1097)
                     /locus_tag="ACHLAG_005343"
                     /pseudo
     CDS             complement(966..1097)
                     /locus_tag="ACHLAG_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1352..>1506
                     /locus_tag="ACHLAG_005344"
     CDS             1352..>1506
                     /locus_tag="ACHLAG_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP0658392.1"
                     /translation="MAFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVA"
BASE COUNT          381 a          316 c          407 g          402 t
ORIGIN      
        1 cgcagaatga agaaatccgg gctggaaaaa ccggaacttg aggcattttt tcgggatatg
       61 accagaggga agcagaagag ctggttgtca cattgtacag acacagaggc gttgattatt
      121 gatcgcgtta tcagtgaggt gcttggggaa tatcccggac taatcaatat tctccggcaa
      181 aggtacgaag gacggggaat gagcaaactg aaaatggcag aaaggttaaa tgcagatcat
      241 cctgagtgga cgttggttac gtgcagacgc cgaattgatc agtggttggg gatatctgaa
      301 tttatgttac atgcccccat gcgtatggct tttgttacag agaaaaaaat gttgcaaact
      361 gatcaataaa ctgcttcaat ccgtataagc ttcgcaaggc tgtatcgcga ggcgaaatgc
      421 aagttttttt cgcacaagga agccaccgga aggtggtttt ttgtgtccgt aatatacagc
      481 agcgcaataa attcgctggt ggttattaat accgttcttt cagcttgctg gctttttcga
      541 caagagttat tggtgtgtca cgttaaccgg aaaagggaaa aagacatgct gaaacagcag
      601 gatatgaccg aaaccgccag agtggtgttt aatgaattaa gcgtcaccga accggcgaca
      661 gtcggggaga ttgcacagaa tacgtacctt tcacgcgaac gctgtcagtt aatactgacc
      721 cagcttgtta tggctgggct ggcagactat cagttcggtt gttacagacg ccttcagtcc
      781 tgaaggcttt tttatttgtg gtaaatgggc ggctgatggg tgtggtggtt gttgctttcc
      841 cgttgctgaa aaagaaagca tcaggcgatt agcagggtat cagttacccg ttgaaatttt
      901 taaatacctc acaattcagg cggttgaatg ttgtctggtt tgcggggagt ttgttaaaag
      961 aaactggcat ggtgaatccc cctgtgcgga ggggcaatca gcaactggtg ttttgtcacc
     1021 gacccttatc ctttctgtgc gggttcaggt gctgatactg aactcaccgg gaggcaaccg
     1081 gcaccatgca atggcacata gcgccactct ccagcccctc tccggagggg ctttcttgtg
     1141 ggcaaaaaaa agcctgagtg ggttcgggca gcagcatgag atatacattt ttataatcga
     1201 atggatttta accagaattc ataaggctgc gcaactgcgc ggtctttttc gaattgcggg
     1261 ctgtcgtctc ccttctgcca ttgtcctgta acttccggac ttcagcccgc tccttatttt
     1321 actcacaata ttatcccggc cgggaggatt tatggcattt aaacactatg atgtggtcag
     1381 ggcggcatcg ccgtcagacc ttgcggatgc gcttgctcaa aaaattcgtg aaggatggca
     1441 accatacggc gggccgtttt cttcgtatac ggatgatggc gcagcactta ttcaggcgat
     1501 tgtcgc
//
LOCUS       DAZGVQ010000352         1494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000352 DAZGVQ010000000
VERSION     DAZGVQ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.356"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..599)
                     /locus_tag="ACHLAG_005345"
     CDS             complement(<1..599)
                     /locus_tag="ACHLAG_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFP0658393.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLEKTLTVN
                     TGALLTMSVSANGGMPPYKYAWKKDGQPVDGQTTDTFSKP"
     gene            complement(666..1010)
                     /locus_tag="ACHLAG_005346"
     CDS             complement(666..1010)
                     /locus_tag="ACHLAG_005346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFP0658394.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGAVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(1007..1453)
                     /locus_tag="ACHLAG_005347"
     CDS             complement(1007..1453)
                     /locus_tag="ACHLAG_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFP0658395.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGTENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIVRMNWAIDEVLRR"
BASE COUNT          338 a          475 c          366 g          315 t
ORIGIN      
        1 ggcttactga aggtgtctgt cgtctgcccg tcaaccggct gaccatcctt cttccaggcg
       61 tatttatacg gcggcattcc cccgttggca ctgactgaca ttgtcagcag cgcaccggta
      121 ttcacggtaa gtgtcttttc cagatttttc acaaacgcca gcggtaccac aaaggacacc
      181 ggtttgcctt tcagacgcag tgagaacgtt gcagccacca cgccgttggt accggatgac
      241 caggtgtgct gacgcacttc cgccaggaat ttaaagccct taccggacgg aaactgcacc
      301 ttaaacgcat acaacgcgtc attgtcataa gcatcacgca gggcgttctg ggcctgattc
      361 agataaaaat tacccgacat ggaaatctcg gacgacgccc ccagaccgtt gatgttctcc
      421 tgctctgtgg agcagagcgt ggtcacatca atatcctgtt tctgaccggc ggtgaactgg
      481 acttccttga tggtgcagtc caggcgcaga tattccgcct tatccatagt ttcagcagtc
      541 gccggggcag atgaaatcat cacctgcgtc agctgtgagc gttcatacaa agcagacatt
      601 ctgcctcctg ataataaaaa acccgcacgc ggcggggtat gggttttgtg ggaaaaaaga
      661 aaaagtcaca ccgtgacctg aaactccagg gttgcacggt aacagcggtt ttccggaata
      721 tagtcctgca tttcactgac ggctcccggg gccagcagca ttatggcttc acgggcgtcc
      781 tgacgtatct gacgcgcctg cgtcacagtc ccggcataaa cgtctatctg caccgacact
      841 gaggactccg cctgcccgcc catcacgtcc gcagacaccg atgaaatcag gctgaaaacc
      901 acccacggaa gcgccaccga cggcctgcca tccagcaggg ggaccacata cgggtacacc
      961 tgcccgccgg caagatgcgc cagatgagga tacaaatccg cctccgtcat cgtctcagta
     1021 cctcatcaat ggcccagttc atccgcacaa tcgccacctg cgctgcctgt tcactgcgca
     1081 catcaaacgc cgggcgcaca aacgggtgcg gtggcatatt cacggtcccc atttccacaa
     1141 accgccagta gaaagcattc cgcgggttat ccgccttcat ggtgttatcg ctgttacctg
     1201 tgtccggatt aacaccacgg atatggacac cggattccat cccgccatcg cgggagcgcc
     1261 gggaaaggac caccacattg cggcgcagtt ttcccctgcg caccggtgcc cgtgacacca
     1321 cttcttcttt cagcacattc gcacccgcac gggttgcctc acgcagcacc cggttatttt
     1381 ccgtaccact cagaagctgc aaatcgcggc tgatgtcctc cagccccgaa aaatccagca
     1441 gggtttcgat catttttccc ctcccagccg acagagaatt tccagacgcc cgcc
//
LOCUS       DAZGVQ010000353         1386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000353 DAZGVQ010000000
VERSION     DAZGVQ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.357"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1272..>1386
                     /locus_tag="ACHLAG_005348"
     CDS             1272..>1386
                     /locus_tag="ACHLAG_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001451419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658396.1"
                     /translation="MLKERSEMFKYAEMLMAEEDDKVCELIFKQQDNLILKI"
BASE COUNT          412 a          248 c          289 g          437 t
ORIGIN      
        1 tgcctcggca tgccagttaa cagctatccg tatggcgtgt tcggttttgc gagtaaaaaa
       61 tggttactgt agaagcggat ttccaccaca tgtcaaccat gaatggtcag tggtgcgaga
      121 cgactgggat gatttggtcg atgacttctt ccggcgaata ctgatagaaa cattgaaata
      181 ttgtaagaga aagcgttcaa aattctgtgg cacgtgaaaa attctacagc gtcatcacct
      241 tattcagtcg accttcaaaa taaacgttta acagagacaa tcaccatttg gattgtccgc
      301 tgaggaggcg aactatttcc tagacagctt tatcatgtct gcccacattg ttggcgctga
      361 caatactaag gacaaacgat ggggaagtag ctttttagcg ttattttcgg gagtaaaaaa
      421 cgtggtatct ttccgtatgt cagttgtatc ttgctggtat tcgtagtcgt gatagagcat
      481 acaacgatat ttatcttgac gttagtgaag aataagttgt tttgtgatct atgttaatct
      541 gggattacaa tccgggcttg atacatcgca tcacgacttt tattctcttc agaatagaat
      601 atgttactcc ccgttttaca ggctcaggat ttgaattaca aattatactt ttaagtgaac
      661 aataaccagg ggtatttctt atttcctgaa acctggtttg gcccatttcg ggatgagttt
      721 gaaaaaactc ttgacgcata cgatagtgat gaaataaacg aaacaaatta cgtcaacaaa
      781 ctcaggcgtc ttgcctgaca tgctcctgac atcattgata ttcacgctca tctggcgaat
      841 gttcatttac agcgtcatca ggatgccgtt atactcattg ataaaatact aacatataac
      901 cctgagaata atcaggggcc acgctggtta ctgggggcgg aattgttacg tgctggcaag
      961 catgacaggg ctctgtacgt attgacagga catgtcaatg aattttcccc ctactggtat
     1021 gagcttgctc ttttacattt tatgaatgag gcatatatga atgctgcaac ggcatttcga
     1081 tatggatttt taacaagcat atattgttga aatgctttgt tagaatcttc atccattccc
     1141 tggggctgta tagtatgatt tttcaggtat ttctgaatac tgccaaatat tattgcaaaa
     1201 cattctctaa attatgaatt aaatatccat aagcattatt acctgggaac tggacatata
     1261 atcactcttc tgtgctaaag gaacgttctg aaatgtttaa gtatgcagag atgttgatgg
     1321 cggaagaaga tgataaagta tgtgaactta tttttaaaca acaagataat ttgattttaa
     1381 aaataa
//
LOCUS       DAZGVQ010000354         1384 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000354 DAZGVQ010000000
VERSION     DAZGVQ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.358"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1321)
                     /locus_tag="ACHLAG_005349"
     CDS             complement(<1..1321)
                     /locus_tag="ACHLAG_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HFP0658397.1"
                     /translation="MHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAESGTVTL
                     RKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSE
                     AVRRVFTKTAQLWHNATPHPQWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPAL
                     YPQVKMVCQMELTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLL
                     NAWSLAGEHRHWMIPLRKGAQYEEIRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARL
                     LTVTRKGKVCHLLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSK
                     KPELVEQELWGVLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGAS
                     PGRIPELMHDLASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          279 a          390 c          358 g          357 t
ORIGIN      
        1 ctgactggct ctttttcggg gctgtggggt atttccaggg cctctccttt accactctcg
       61 ggaaggccct ttcccttctt gtcggtaatt tcacaagttg tcccatactt gcaagatcgt
      121 gcatcagctc cggtatacgt cccggtgaag cgccctgcaa tgtcatcagc attctcatca
      181 ccattccgca tgattctgag aaactcagtt gattcggcca gtaacctttc agatgttccg
      241 ccattttaat catctgatat ctcaccagat tataagccag taagacaccc cacagctctt
      301 gctccacaag ctccggcttt ttacttctca gcgtcagcct gctcagttgc atcgtctgtt
      361 ttatctccct gtatcccagt tcgatttccc agcgatgact gtacagatcc gccatttctc
      421 ctccggggaa gcgcatggcg tccgtcatcg acgtcagcag atggcagact tttcctttgc
      481 gcgtcacggt cagcaggcgg gctgtcacct catttcccag tcccggccac ttttttcgtg
      541 cctgcgggct ggttttcagc ttcaccagat gatcgccttt acccagtttt ctgatctctt
      601 catattgcgc tccctttctg agagggatca tccagtggcg gtgttctccc gccaggctcc
      661 aggcatttaa cagtcccagt gagtaataac ctttgtccat taacgtcaga gtgttatcgc
      721 cggtttgttc tataagttgc tcagcaagct cattttcgct gttcttcatc gtgccgaagg
      781 ctgcagccgt cagcagatgg ctggtcagtt ccatctggca gaccattttg acctgcgggt
      841 agagcgccgg gttcccggca tgtgtctggc gggggaaggc tgcatcgttc tctggtgtat
      901 ccggtgtgcg ccagaacaca ccatcgatgg ccagcagggt caggccgcac cattgcggat
      961 gcggcgtggc gttatgccag agctgcgctg ttttcgtgaa cacgcggcgg acagcctcac
     1021 ttcccaggcg ctggcgggcc tgaataacgg cactgggggc aacgaagggg cgattgcccg
     1081 gcagcatgat gtccaggcga ttcacaatct ggtgaagagg ttctttacgc tcaagcgcca
     1141 tgccaacaat acaccagacc atcatttcga ggggaagacg gcgcttgcgt agcgttacag
     1201 tacctgattc ggcaaggcaa cgagagatga gttcggggtc gaggtaatcc cccagagaag
     1261 tcagtgggtt acgcagagaa tcgtaacggg ataccaaatc aagggcctgt ccaatgtgca
     1321 taaaaaaatc cggaaacgag tgagcgtttc cggattctta cacagccact ggatcggtca
     1381 actg
//
LOCUS       DAZGVQ010000355         1372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000355 DAZGVQ010000000
VERSION     DAZGVQ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.359"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(27..773)
                     /locus_tag="ACHLAG_005350"
     CDS             complement(27..773)
                     /locus_tag="ACHLAG_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HFP0658398.1"
                     /translation="MKKIIAVSALAVVGMFSGWALADRGKTGFYMTGKAGASVVTQTD
                     QRFRQDFGDDVYKYKGGDKNDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGAADSRY
                     TLDTWYSPMVGGGREDTQNRLSVNTLMVNTYYDFRNSSAFTPWVSVGLGYARIHHKAT
                     YTDTSWNKSGEVSDFSALHYPGYDNNFAWSFGAGVRYDVTPDIALDLSYRYLDAGDAS
                     VSYKDEWGDKYKSEVDVKSHDIMLGVTYNF"
BASE COUNT          385 a          353 c          285 g          349 t
ORIGIN      
        1 attattatct ttcaggagta gtttcgtcag aagttataag tcacaccaag catgatgtca
       61 tgacttttaa catcaacttc tgatttatat ttatcgcccc actcatcctt gtaactcaca
      121 ctggcatcac ctgcatcaag atagcgatag ctgaggtcca gagcgatatc cggggttacg
      181 tcatagcgga cacctgcccc aaaactccat gcaaaattgt tatcatatcc cgggtaatgc
      241 aacgcagaaa aatcgctcac ctcaccagat ttattccagg aggtatcagt atatgttgcc
      301 ttatgatgta tccgtgcata acccagtcca acagacaccc atggagtgaa tgcactgctg
      361 tttctgaagt cataatacgt attcaccatc agagtattca cactgagcct gttctgtgtg
      421 tcttcccgac caccaccgac catcggagag taccatgtat ccagtgtata acgggagtcc
      481 gcagctccgc ggccatagaa ttccacttcc gtgcgcacag ggacattgta atgctgataa
      541 aaatcatagc ccaccgcaag gccggcaccg aataccgtat cgttcttatc gccgccctta
      601 tacttataaa catcgtcccc aaagtcctga cgaaagcgct ggtcagtctg agtcacaacc
      661 gaagcaccag ctttaccggt catataaaag cctgttttgc ctctatcagc cagggcccag
      721 cctgaaaaca tgcccaccac agcaagcgct gaaaccgcaa taatcttttt catttcagtt
      781 atctccgcta tcactttccc cgaacgataa acgtggttgc ttattctaaa gctatgcaaa
      841 agacagggta aaaagagaaa aatcgatcga atcgatcgac aaaatcgatc atgcatgatg
      901 aagatttctt atcgaataca taaaaatagt gatggcaatc gtggcaggag cttacgtcag
      961 gccctgaagt actgacacag gcattgatgg caggtgatat aaggagtgct ccagggaatg
     1021 actcccgact gacccaggca ctgtcccgtt gtcaacccgt atggttactg ctctgactca
     1081 tttcctgtcg tcagacgttc aactctggac cactggttat gacgacaggt gtcgctcatt
     1141 tgtcagcact ataacggtca aagatcgtgg cattgacaca ttctccagag gtaaatttct
     1201 caagaggtaa caactctgcc aggaggatat ctgcagagag ttacattcgt ttgggttcat
     1261 atctgtgccg cgcgttttcc ttccatcagt gaacaaacat tcagtcccat gacgaccggg
     1321 caaatagcgt gttcacagat attgctgtcc aggtacgaag taccataatg ga
//
LOCUS       DAZGVQ010000356         1344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000356 DAZGVQ010000000
VERSION     DAZGVQ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.360"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1044)
                     /locus_tag="ACHLAG_005351"
     CDS             complement(<1..1044)
                     /locus_tag="ACHLAG_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP0658399.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVQPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDI
                     RLQIRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAVQPDGHTLARLWA
                     SLPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGMADRFGR
                     TLAYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKPHTASLSSPDSPRPL
                     SAPSFPDTLPGTEYGADSGIRLSAVWLMHDPEYPENLPAATLVCYDWTPRGELAAVYD
                     RSGTQMRHFTYDDK"
BASE COUNT          294 a          405 c          351 g          294 t
ORIGIN      
        1 tttatcatcg taagtaaagt ggcgcatctg cgtaccgctg cggtcataca ccgccgccag
       61 ctcaccgcgt ggcgtccagt cgtagcacac cagtgtcgcg gcaggtaaat tttccgggta
      121 ttccgggtcg tgcatcagcc acaccgccga aagacggata ccgctgtcag cgccgtattc
      181 cgtaccgggc agtgtgtcgg ggaaggaagg agctgagaga gggcggggac tgtcagggga
      241 agataacgaa gcggtgtgcg gtttacgggc ctcttccgcc cgctgtgcct gtgtggtcag
      301 taccagacgg aactcccgcc cggcaccgtc cgtcacgccg gtgatttccc cggccaggtc
      361 accggcggcc tcacgccggt acgccagcgt ccgtccgaag cggtccgcca tcccggtaag
      421 cacccggtac ggcggcagcg gcgctggcag tacgtcctca gcacccggca cccgctcaga
      481 ccaccccagt atccaccacg gcccctgtgc gctgttggtc gccaggtaaa gatgcgggct
      541 taaccggata tccggcggca gcgacgccca cagccgtgcc agcgtgtggc cgtccggctg
      601 cactgcctta ccgccacgca ccagccacat tgactcactg cggctgtaca ccgcctcccc
      661 cggcagcagc ggctcaaagt gaatgctccg cccgccgttg tcgttgagta ccagtgcatc
      721 atcgcgtatc tgtaagcgga tatcagaagg cgctttccag ccggggccga aaacgcccac
      781 cggtgcaggc gtcttcgtcc ggtagctgct gtaggtgcgg gagagaatga acggcagcgg
      841 gccgggcagc gcaaggtccg tctcgccggg ctgtactttt gcaccgagca gcgggttcac
      901 cgggttgccc gaggtcatcc cgcccggaca caccgagcac gccacgccag ttggcgcgcc
      961 aattcttaca cctgccgaac cctggacaat gggaccgcca tactgagtca tatctccctg
     1021 acgcgccgct ggttttccgc tcatataaag tgccctttta gttattgttc atatccctgt
     1081 gttagcaaca aaaaaaataa aatgacacac aagaccagct aatgtattta tatatttttc
     1141 acataaactt atttgttctc aatcaacaac aactgttatt acaggtcact ttcaatattt
     1201 tattaaacta aaatttttag cacagatgaa ttcatcatca acaaattcac tatatggata
     1261 atatccattc aacgcatatg atttatgatt ttattagtct gagttatttt tgtagggcta
     1321 tatttaaaat tattaaactc cacc
//
LOCUS       DAZGVQ010000357         1340 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000357 DAZGVQ010000000
VERSION     DAZGVQ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.361"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            192..1235
                     /locus_tag="ACHLAG_005352"
     CDS             192..1235
                     /locus_tag="ACHLAG_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP0658400.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAVGMKHSSIQHQ"
BASE COUNT          389 a          286 c          298 g          367 t
ORIGIN      
        1 tttagaattt caatcatctg acatcattat aaaaaatgag attcacataa agataaaatt
       61 attgttctca aggaaagcat gaaggggctt cgtaagcaat attcattcgg tgccaggaca
      121 ggacttgata cacagaatct caacttttat gctctcagac tatatcacca catgttttac
      181 aggctcagaa aatgaacgat agattatgct ttgaagtgca cgataacaag ggttatttcg
      241 tatttccgga tacctggttt ggcccattgc tgggggagtt tgaagaagtg cttgatgcat
      301 acgacgctga tgaaatcagc gagacgagtt acatcaacaa actccgacgc cttgctcaac
      361 gggagccgga ctttattgat atacacgctc atctggcata tgcatttctg gaacagaatg
      421 ccccgcgtaa ggcactgaac gccgctctta aaggactggc ggcaggtaat cgtatcatcc
      481 cggaaagttt ctgtggtgaa ataatctgga tgcatcccga aaaccggcct tatctgcgcg
      541 cgctttatgc agcaattctc gcaaatgtgc acttacagcg ccatcaggat gcggttatgc
      601 tcactgataa aatactggca tacaatcctg aagataatca gggggctcgc tggttgttgg
      661 gttcagagct gttacgcacc ggcgatcatg agcgagcctt cagtgtactg aaagagcatg
      721 ctgatgaatt ttcaccttac tggtatgaac tcggtctcct gcattttctg aacggggaac
      781 atgttaaggc cgccacagct tttcgccatg gctttgccac aaacacttac atcgcagaaa
      841 tgctgtgcgg aaatctgcat ccatttccac ttgctgtgtg gcatgacttc tccggctctc
      901 tggatacggc agaagattac tatgcaacat actcaccatt atggggacaa tatccggaag
      961 cattgctgtt tgtaaactgg ctctataacc attcctccgt gctgcatgaa cgagcagaaa
     1021 tcattaaatg cgccgaaatg ctgattcagg aagatgattt cgaaatatgc gaaagtattt
     1081 taagacagca ggagcatctg tggaaaagaa ttgacaagac actttctgag gaaattgtac
     1141 agaaatgcag aaatatgaac ggtgaatata tctggccctg gatattgccc ttttccgcag
     1201 tagggatgaa acattcaagc atccagcatc agtagatatt gcattagtgt aactttaaaa
     1261 caagtaataa tttattatcc aataataaca ctgtgttcct tccccaggtg attaccctgg
     1321 ggttattttc tgaaagtttc
//
LOCUS       DAZGVQ010000358         1327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000358 DAZGVQ010000000
VERSION     DAZGVQ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.362"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..237)
                     /locus_tag="ACHLAG_005353"
     CDS             complement(<1..237)
                     /locus_tag="ACHLAG_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP0658401.1"
                     /translation="MLPILWLPSARDDLRQIVAYIAKENIPAARRLKIRIETSVLALS
                     EHPYLYPPSDRVSGLREIVVHPNYIVLYRVAASSI"
     gene            complement(237..428)
                     /locus_tag="ACHLAG_005354"
     CDS             complement(237..428)
                     /locus_tag="ACHLAG_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFP0658402.1"
                     /translation="MSTALSPIVSEFETTEQENSYNEWLRAKVASSLADPRPSIPHDE
                     VMAEMENLIAQIAVTNRSE"
     gene            complement(456..857)
                     /locus_tag="ACHLAG_005355"
     CDS             complement(456..857)
                     /locus_tag="ACHLAG_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0658403.1"
                     /translation="MMNALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVTGHPLHWFLLPPEEGEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            967..1239
                     /locus_tag="ACHLAG_005356"
     CDS             967..1239
                     /locus_tag="ACHLAG_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0658404.1"
                     /translation="MLDSTREKIRQKYTQAEIGRYMGVAQQTVWQWFSFDVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVGFKVNTSNAPELIHENQA"
     gene            1223..>1327
                     /locus_tag="ACHLAG_005357"
     CDS             1223..>1327
                     /locus_tag="ACHLAG_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658405.1"
                     /translation="MKIKHEHIRMAMNAWLLYPRVGRKKIADDIATAYF"
BASE COUNT          360 a          285 c          292 g          390 t
ORIGIN      
        1 aatgcttgaa gctgctactc ggtacaaaac gatataatta gggtgaacca caatttcccg
       61 caaaccggat actcgatcac ttggcggata tagatatgga tgctcagaga gggctaaaac
      121 agacgtttca atccgtattt ttagtctgcg tgctgcagga atattttcct tagcaatata
      181 ggctacgatc tgacgcaaat catcgcgagc agatggtagc cacaaaatgg gtaacattac
      241 tcgctcctgt tagttacagc aatttgagca ataagattct ccatttcagc cattacctca
      301 tcatgtggaa ttgagggacg agggtcagca aggcttgacg ccactttagc gcgtaaccat
      361 tcgttgtaac tgttttcttg ttcggtagtt tcgaattctg aaactatcgg agaaagggct
      421 gtactcatgg cataactcct cttcttgtac tgtggtcacg cccggcggct tttttgtgcc
      481 gccaaccacc gggcaatggt ttcttccatt gattttttct tgtctttgat ttcttgaagc
      541 attttttctt ggtcttcctc tggaaacgca ctaaaagcct ggagcagttc gcgttgacga
      601 ggaccaattt tcatcgtgtc aggggtgaaa atttgctccc cctcttcagg aggtaataaa
      661 aaccaatgca atggatgccc tgtaacctca accagtttat ccaaacttga ggctttaggt
      721 gtagccttac cgctgaccca ttgttgaaca gtttgttgtg tcacaccaat tctacgggca
      781 agctcagcct ggctccatcc agtttcctga agaagcttgc tgattctgta catagatact
      841 tctagggcgt tcatcattat tcaattttac aggtaaatac tgttaaaagc atcacaataa
      901 aaaactgttg attgtgtaca gttttttatt gtaggctttg cttatagttt tttagaggag
      961 agcaaaatgc tagatagcac tcgcgaaaaa attaggcaga aatacactca agctgaaata
     1021 ggtcgttata tgggggtcgc tcaacagaca gtttggcaat ggtttagctt tgacgttccc
     1081 ccaaagcagg taattccgtt atgtcagcta atgaagtggg aagttacccc gcatgaaatt
     1141 cgccctgata tttatcctaa cccaaccgac ggtttacctg ttggattcaa ggttaacaca
     1201 tcaaatgcac cggagttgat tcatgaaaat caagcatgaa cacatccgca tggcgatgaa
     1261 tgcgtggctg ctttatccga gggtagggcg caaaaaaatc gctgatgata tagcgacagc
     1321 atatttt
//
LOCUS       DAZGVQ010000359         1325 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000359 DAZGVQ010000000
VERSION     DAZGVQ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.363"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1001
                     /locus_tag="ACHLAG_005358"
     CDS             <1..1001
                     /locus_tag="ACHLAG_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFP0658406.1"
                     /translation="SNFVELRKVAGAMATLEGLTRTYFSTPSGETHEAYQFWTGDRWN
                     SEQPRSKRSEFDVSHAHLKGGVLFPDETWRQIVTIEDAVANGWRYTNIGVIRSENTES
                     EFRNLYMCEFVRDGEAAFSLSALTGCGVDGYDEWPDWKPFAARPMGVREVWLGYDANG
                     SSGKGDCGALSVCVPPLVAGGRFRTVETVRVQGMEFEQQAALIEEYTQKYNVKHIGID
                     VTGGNGEGVYQIVKKFFPMAVPYNFNIASKRALVMKMLQVVRAGRWEYDRSERDLIQA
                     FASVRKIQTPGGYITYDTDRSRGVSHGDLAWATMLAVINEPIGAEREDNRGFAMEF"
     gene            1007..>1325
                     /locus_tag="ACHLAG_005359"
     CDS             1007..>1325
                     /locus_tag="ACHLAG_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537153.1"
                     /note="gpQ; Q protein; structural capsid protein;
                     bacteriophage P2-like virions include a head and a tail; Q
                     is a connector or portal protein that joins phage head and
                     tail; Q, P and M proteins are needed to package DNA into
                     proheads and for the conversion of proheads to capsids;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP0658407.1"
                     /translation="MSKRKKYTGKRGQDSLHDALKSTPALSAFTFDGPYEVSHMDLLD
                     NMSCADNGRYFDTPMDWNAIARASRRASWHQSALYFKRNALNGCFVPHPLLSRQAFSA
                     FALD"
BASE COUNT          302 a          332 c          394 g          297 t
ORIGIN      
        1 tcagtaactt tgtggaactc aggaaggttg ctggggcaat ggcgacactg gaagggctta
       61 cgcgaaccta cttttcaaca ccgtcaggtg aaacacatga ggcgtatcag ttctggacgg
      121 gggaccgctg gaacagcgaa cagccacgct caaaacgcag tgagtttgat gtttcacatg
      181 cgcatctgaa ggggggcgtg ctctttcctg acgaaacatg gcggcagatt gtcactattg
      241 aggatgctgt cgctaacgga tggcggtaca ccaatattgg tgttattcgc agcgaaaata
      301 cagaaagtga atttcgtaat ctgtacatgt gcgaatttgt ccgtgatggt gaggcagcct
      361 tcagcctgag tgcgctcacc ggctgcggtg ttgacggcta tgacgaatgg cctgactgga
      421 aaccttttgc tgcccgccct atgggggtga gggaggtctg gcttgggtat gacgccaacg
      481 gcagcagcgg gaagggggac tgcggggcac tgtcggtgtg cgtgccgccc cttgttgccg
      541 gtgggcgttt tcgcacggtg gaaactgtca gggttcaggg gatggagttt gaacagcagg
      601 ccgcactgat tgaggaatac acccaaaaat ataacgtgaa acatatcggc attgatgtga
      661 ccggcgggaa cggggagggg gtttatcaga ttgtgaaaaa gtttttcccg atggccgtac
      721 cgtacaactt caatatcgcc tccaagcggg cactggtcat gaaaatgctt caggtggtgc
      781 gtgccggacg ctgggagtat gaccgcagtg aacgtgacct gatccaggca ttcgcatcgg
      841 tgcgaaaaat acagacaccc ggcggctata tcacctacga caccgaccgc tcccgtggcg
      901 tcagccatgg tgacctcgcc tgggcaacca tgcttgctgt tatcaatgag ccaattggtg
      961 cggagcgtga ggataatcgc ggctttgcaa tggagttctg aacacaatga gtaaacgcaa
     1021 aaaatacacc ggaaagcggg ggcaggattc gctacacgat gcactgaaat caacgcctgc
     1081 actcagcgca ttcacctttg atgggcccta tgaagtcagc cacatggatc tcctggataa
     1141 catgtcatgt gcagataacg ggcgctactt tgataccccc atggactgga acgctatcgc
     1201 ccgtgcatcc cgtcgtgcgt cctggcatca gtcggctttg tacttcaagc gcaacgccct
     1261 gaacgggtgc tttgttcctc accctctgct gtcccgccag gcgttttcag cctttgcgct
     1321 cgact
//
LOCUS       DAZGVQ010000360         1321 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000360 DAZGVQ010000000
VERSION     DAZGVQ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.364"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..301
                     /locus_tag="ACHLAG_005360"
                     /pseudo
     CDS             1..301
                     /locus_tag="ACHLAG_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
     gene            complement(543..803)
                     /locus_tag="ACHLAG_005361"
     CDS             complement(543..803)
                     /locus_tag="ACHLAG_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658408.1"
                     /translation="MDYPNLPKQTFADLIALRQAVVALINVLPDEEKELVKAILNRTA
                     ADFSSFPLTDDLADLPELIAASAIKLTEEIYSPQKPSQNSCE"
     gene            915..>1321
                     /locus_tag="ACHLAG_005362"
     CDS             915..>1321
                     /locus_tag="ACHLAG_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0658409.1"
                     /translation="MNAKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGA"
BASE COUNT          360 a          282 c          356 g          323 t
ORIGIN      
        1 ttacccggat aagattactg ttactgtggc actggaaatt gccgggcagc cgtcgttgtt
       61 gtcgccatat gtggcgatta gtcttgaccg tactgaaggt gccacgctgg aattttacga
      121 agctgaggcg aaaaaacagg cgaagcagtt tttcatggat attgctgccg ctttatgtga
      181 aggggatgag cagtcgccgg aaaagcgccc cataatttta gaggcgcagg atgtgttgat
      241 aacctacaga ggaaaactac cgggaataat tactggttct ctgaagacgc caccgacggc
      301 attgcggtca gaaaacgatg atattgaatc acgcattgaa aaactggagt gctatatcgc
      361 tgaattgaaa aaaagcaccc caacaaaaaa tgaggtgctt gcagcagatg aaatgaaaga
      421 aactattctt gatcgcgcgg cgcatctaag ctgcgcttca cggttgaaag agcatcttca
      481 gcggcctgaa gaaaaacgcc gcgatgagca gttcgcggcg ttttacgatt attgcattga
      541 agttactcgc aggaattttg tgaaggcttt tgaggagagt aaatctcttc agtaagctta
      601 atggcggacg ctgcaattaa ttcaggaagg tccgcaaggt catctgtcag tgggaatgat
      661 gaaaaatcgg cggcagttct gttaagaatt gctttaacta attccttttc ctcatccggc
      721 aacacgttga ttagagctac gactgcttgc ctgagtgcga ttaaatcagc aaaagtttgt
      781 tttggtagat ttggataatc catagtcacc tctgtgttta tcagattgac ataccctggc
      841 cagtgcccac cactggcggg gtgaaggctt aacatatcca gggattcgga accgataaat
      901 cctgataaat atccatgaac gcaaaaatca aatacggcct gtcggctgcc gttctggcgc
      961 tgattgccgc aggggcttct gcgcctgaaa ttctcgacca gtttctggat gaaaaggaag
     1021 gtaaccacac cacggcatac cgtgatggtg caggtatctg gaccatctgc cgtggtgcca
     1081 tcatggtgga tggcaaacct gtcgttccgg gcatgaagtt gtcgaaggaa aaatgcgacc
     1141 aggttaacgc cattgagcgt gataaagcgc tggcgtgggt ggagaaaaac atcagagtgc
     1201 cgctgaccga accccagaaa gcggggatcg cgtcattctg tccgtataac attggtcccg
     1261 gtaagtgttt cccgtcgacg ttttataaac gaattaatgc aggcgatcgc aggggagcgt
     1321 g
//
LOCUS       DAZGVQ010000361         1310 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000361 DAZGVQ010000000
VERSION     DAZGVQ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.365"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..940
                     /locus_tag="ACHLAG_005363"
     CDS             <1..940
                     /locus_tag="ACHLAG_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP0658410.1"
                     /translation="SGNAKDYQFSYSNNWQHISYTFSASQSYDENNKEEERFNLFISI
                     SFYWGDDIAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVNQQQQNN
                     DTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWSGGINITNQLSDTFAIL
                     DAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQGNRQIIA
                     PYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGSRLFIRV
                     DEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            953..>1310
                     /locus_tag="ACHLAG_005364"
     CDS             953..>1310
                     /locus_tag="ACHLAG_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFP0658411.1"
                     /translation="MKWLLLITLSLYSFIVQSAPCALTNVGEQRGTYILKPLSMKGNL
                     TAEKIENTGNIIECTDLQETTNAIKVHFLTQNALRFKVGSRHYIINFPFEYDGKKIEL
                     PGKSSYNLEEMLSAINF"
BASE COUNT          432 a          249 c          249 g          380 t
ORIGIN      
        1 aagtggaaat gctaaagatt accaattcag ttactccaat aactggcaac acattagtta
       61 tactttctct gccagccaat cttatgatga aaataataaa gaagaggagc gttttaatct
      121 gtttatctcc atttctttct actgggggga tgatattgcc aaaacacgtc accaaattaa
      181 cttatcgaat tcgacctcat tttccaaaga tggctattcc tccaacaata ctggaattac
      241 tggcatagcc ggtgaacatg atcagttaaa ttatggtata tatgttaatc agcaacaaca
      301 aaataatgat acctcgcttg gtacgaattt aagctggaga actcccatcg ccataataga
      361 tggcagctat agtcattcta aaaacgcctg gcaaagtggt ggaagtatta gtagtggatt
      421 agttgtctgg tccggtggta ttaatatcac taaccagtta tccgatacat ttgcaattct
      481 ggatgcgcct ggattagaag gtgcacatat taatggacaa aaatacaatc gaacaaacag
      541 caaaggccag gttgtttacg acctgataat acctcatcgt gaaaaccatc tggtacttga
      601 tatagcaaac agtgaaagtg aaacagaatt gcagggcaat cgtcaaatta ttgcacctta
      661 ccgtggagca gtttcttatg tgcagtttac aactgaccaa cgtaagccct ggtatataca
      721 ggcactgcgt cccgatggtt cgccattaac ctttggctat gacgtactgg atctccagga
      781 aaataatatt ggagtcgttg gccagggtag tcgccttttt attcgcgtag atgaaattcc
      841 cactggaata aaagttgctc tcaatgatga acagaactta ttctgtacta ttacttttca
      901 acacgttatc gatgaaaaca aaacatatat atgccagtaa aaggagttta ctatgaagtg
      961 gcttttatta atcaccttga gtctatattc ttttattgtg caaagtgctc cctgtgcatt
     1021 gaccaatgtc ggtgaacaac gtggaacata tatactcaag ccactgagta tgaaaggtaa
     1081 tctaaccgcc gaaaaaattg aaaacaccgg aaatatcatt gagtgtacag acttgcaaga
     1141 gacaaccaac gccattaaag ttcatttttt aacacagaac gctttaaggt ttaaagtcgg
     1201 aagtcgtcat tatattatta atttcccctt tgaatatgac ggtaaaaaaa tagagcttcc
     1261 agggaagtca tcttataatt tagaagaaat gctatctgcg ataaatttca
//
LOCUS       DAZGVQ010000362         1305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000362 DAZGVQ010000000
VERSION     DAZGVQ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.366"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            331..867
                     /locus_tag="ACHLAG_005365"
     CDS             331..867
                     /locus_tag="ACHLAG_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0658412.1"
                     /translation="MQTQNVITEKEREIRSRDLTENAKKLAHERREAEKNSNFTQVSP
                     LGWKRLMELAKGNQGAFGLYSFFAQHIDGSCGAVVADQSFLAAQFNVSTRTIIRWINY
                     LEENRAVVRIPVAGKVCAYALNPNEVWKGYGNTKEYAAFITKTLVNKDGDIQRRIMAM
                     FHGNDARQGMEGDTPEAE"
BASE COUNT          375 a          296 c          319 g          315 t
ORIGIN      
        1 aaaaaaatcg gaacccctga aggggccccc acgatttttc ggttgccaat ggttaaattt
       61 tcgccgtttg cccgactgaa aggagggtgc taccaggaga tctcgcagca cgctgtgaga
      121 tctctttaga taagaatagc aaaaattaat aattcattga tttaaaagac atttaacatt
      181 tctgatatga caccctagga tgtcacaacg ttatgacacc ctaggatgtc acatcatttc
      241 gtaatatgtg acataaatgg catatactac gacataaaat gtcgtatagt atgacttgtc
      301 ttgtcacatt gaaatgacat ggagattgag atgcaaactc aaaatgtaat caccgaaaaa
      361 gagcgagaaa ttcgatctag ggatctaacc gaaaatgcca aaaaactggc tcatgagagg
      421 cgagaggcag aaaagaactc aaatttcaca caagtgtccc cattaggctg gaagcgttta
      481 atggaattag ccaaaggaaa tcagggagct tttggcttgt attctttttt tgctcagcat
      541 atcgatggat cttgtggcgc agtagtggcg gatcagtcat ttttggcggc gcagttcaat
      601 gtatcgacaa gaacaataat tagatggatc aactatttag aggaaaacag ggctgttgtg
      661 aggattcctg tcgccggtaa ggtttgtgca tacgcattga atccaaatga agtttggaag
      721 gggtacggga ataccaagga atatgcggcg tttataacaa aaaccctagt caacaaagac
      781 ggcgacattc agcgccgaat catggccatg ttccacggaa atgatgcccg tcagggcatg
      841 gaaggcgaca cgccagaagc cgaatagatt cccgttcgcc aaatcgccga agcccgcccg
      901 caagggcggt acaggttcca cagggagctg cttcaccccc tccgccctgc aaagccctaa
      961 aacgctctca gaacgcacgt aagccatttt tccgcccaaa gcccacgtga gcccgtagaa
     1021 cgtcaaaaaa cgcgtcagag cgcgttttag ggcgttttac gggtgatttc gtgggtcttg
     1081 ccggactggt tcaccaggac ggctagaggc ggagtactcc gattatccag actggtttca
     1141 tctctcaaat tcgtgaaatt agagagatgc gcacttatat gtttcttcgc cgttggctca
     1201 gaaacatgtg cggcctgcgg cgctaaaacc cgtctggcag gtggttgcgc tcactgcgtg
     1261 aacacaacca ccttccccaa tttttttgat cggtgcgttg gcaac
//
LOCUS       DAZGVQ010000363         1289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000363 DAZGVQ010000000
VERSION     DAZGVQ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.367"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..249
                     /locus_tag="ACHLAG_005366"
     CDS             <1..249
                     /locus_tag="ACHLAG_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFP0658413.1"
                     /translation="EMLQDKNEEVRIEAIIGLSYRKDKRVLSVLCDELKKNTVYDDII
                     EAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDKLKRS"
     gene            277..1235
                     /locus_tag="ACHLAG_005367"
                     /pseudo
     CDS             277..1235
                     /locus_tag="ACHLAG_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          378 a          254 c          324 g          333 t
ORIGIN      
        1 gagatgcttc aggataaaaa tgaggaagtc cgtattgaag caataatcgg actttcctac
       61 agaaaagata aaagggtttt atctgtttta tgcgatgagt taaaaaaaaa tactgtttat
      121 gatgatatca ttgaagctgc gggtgaatta ggtgataaaa cgctacttcc tgttttagat
      181 actatgttgt acaagtttga tgacaatgaa attataactt ccgctattga taagctgaag
      241 cgttcatagc gtgagttgcc tatgcacagt gggggattcc cgccggcacg gtgcagatgc
      301 tcgaccggct ggaaagtgaa atcctggctg accgggtgag tgaggaaagc cgccgctggc
      361 tggcatcgtg cggcctgacc gtggagcaga tgaaaaacca gatggacccg gtgtacacgc
      421 cggcgcgaaa aatccacctg taccactgcg accatcgcgg cctgccgctg gcgctcatca
      481 gcacggaagg ggcaacagcg tggtgcgcag aatatgatga atggggcaac ctgctgagtg
      541 atgagaaccc gcatcatctg cagcagctca ttcgcctgcc gggtcagcag tatgatgagg
      601 agtccggcct gtattacaac cgccaccgct attatgaccc gctgcagggg cggtatatca
      661 ctcaggatcc gattgggctg aaggggggat ggaattttta tcagtatccg ttgaatccgg
      721 tcataaatgt agatccgcaa ggtttggttg atataaattt ataccccgaa agtgatctta
      781 tccattctgt agctgatgag attaatatcc caggcgtttt cacaatcggg gggcatggta
      841 cccccacatc tattgaatcc gcaacgcgca gtatcatgac agctaaagat ctagcatatc
      901 taattaaatt tgatgggaat tataaagatg ggatgacagt ttggttattt tcttgtaata
      961 caggtaaagg acaaaattca tttgctagcc aattagctaa agagttacat acaaatgtaa
     1021 taggacctga cacgctatgg acgtggtggg ggcgaggaac taatggtaag ttaaaaatgg
     1081 atacagtgct aacagcacca acgaacctta attcaaataa ggatctaatg gctataacaa
     1141 caaaagacct tggtaattgg ataacatatg ggccatctgg gcaccccatt tctaatatgc
     1201 aaggtacgcc agaaaaaccc agtgatataa gataggttgt agatgtatga aagcatgctt
     1261 gttactattt ttttattttt ctcttattg
//
LOCUS       DAZGVQ010000364         1285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000364 DAZGVQ010000000
VERSION     DAZGVQ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.368"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1281
                     /locus_tag="ACHLAG_005368"
     CDS             <1..1281
                     /locus_tag="ACHLAG_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP0658414.1"
                     /translation="RVRPDMAQNNADRLVIDGGRATGKTILNLVNAGNSGTGLATTGK
                     GIQVVEAINGATTEEGAFVLGNMLQAGAFNYTLNRDSDESWYLRSEERYRAEVPLYAS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNL
                     THTSSGLWADIVAQGTRHSMKASSGNNDFRARGWGWLGSLETGLPFSITDNLMLEPRL
                     QYTWQGLSLDDGKDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAK
                     HSVRELPVNWWVQPSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSINGSSAEGYNSQATLNVTF"
BASE COUNT          274 a          356 c          402 g          253 t
ORIGIN      
        1 cgtgtacgcc cggatatggc gcagaacaat gctgacagac tggtcattga cggtggcagg
       61 gcaaccggaa aaaccatcct gaatctggtg aacgccggca acagtggtac ggggctggcg
      121 accaccggta agggtattca ggtggttgag gccattaacg gtgccaccac ggaggaaggg
      181 gcttttgtcc tggggaatat gctgcaggcc ggggccttta actacaccct caaccgggac
      241 agtgatgaga gctggtatct gcgcagtgaa gaacgttatc gtgctgaagt ccccctgtac
      301 gcctccatgc tgacacaggc aatggactat gaccggattc tggcaggctc ccgcagccat
      361 cagaccggtg taaacggtga aaataacagc gtccgtctca gcattcaggg cggccatctc
      421 ggtcacgata acaacggcgg tattgcccgt ggggccacgc cggaaagcag cggcagctat
      481 ggcttcgtcc gtctggaggg tgacctgctc agaacagagg tcgccggtat gtcactgacg
      541 actggagtgt atggtgctgc aggccattct tccgttgatg ttaaggatga tgacggctcc
      601 cgcgccggca cggtccggga tgatgccggc agcctgggcg gatatatgaa cctgacacac
      661 acctcctccg gcctgtgggc tgacattgtg gcacagggaa cccgccacag tatgaaagcg
      721 tcatcgggca ataacgactt ccgcgcacgg ggctgggggt ggctgggctc actggaaacc
      781 ggtctgccct tcagtatcac tgacaacctg atgctggagc cacggctgca gtacacctgg
      841 caggggcttt cgctggatga cggtaaggac aacgccggtt atgtgaagtt cgggcatggc
      901 agtgcacagc atgtgcgtgc cggtttccgt ctgggcagcc acaacgatat gacctttggt
      961 gaaggcacct catcccgtgc ccccctgcgt gacagtgcaa aacacagtgt gcgtgaactg
     1021 ccggtgaact ggtgggtaca gccttccgtt atccgcacct tcagctcccg gggtgatatg
     1081 cgtgtgggga cctccaccgc aggcagcggg atgacgttct ctccctcaca gaatggcact
     1141 tcactggatc tgcaggccgg actggaagcc cgtgtccggg aaaatatcac cctgggcgtt
     1201 caggccggtt atgcccacag catcaacggc agcagcgctg aaggttataa cagtcaggcc
     1261 acgctgaatg tgaccttctg ataat
//
LOCUS       DAZGVQ010000365         1271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000365 DAZGVQ010000000
VERSION     DAZGVQ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.369"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(23..103)
                     /locus_tag="ACHLAG_005369"
     CDS             complement(23..103)
                     /locus_tag="ACHLAG_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658415.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(139..563)
                     /gene="yqeK"
                     /locus_tag="ACHLAG_005370"
                     /pseudo
     CDS             complement(139..563)
                     /gene="yqeK"
                     /locus_tag="ACHLAG_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
     gene            757..1247
                     /locus_tag="ACHLAG_005371"
                     /pseudo
     CDS             757..1247
                     /locus_tag="ACHLAG_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          467 a          224 c          183 g          397 t
ORIGIN      
        1 ataacaaaaa gatggaacct cgttaatcat aaaacaataa aattaaatat tctcgcagta
       61 tatggcagtc taaagcatca aagttttgat caacatcttt cattttagac atctccttgc
      121 aatgtaaaat atcatgtatc aacaacatct ggtgcaaatc catgagtcga actcgacatt
      181 catcttagct cgattattat tccttcgtac agtcgatgta aacaatacag aaagaggatt
      241 attaagaacc agtttactca gtgaatcaat agaggaaagg attaacgttt ctttaaaaga
      301 attaatttca tcatctgtta aactaaactc atcattgaca gatcgtgaga tataactgtt
      361 tttaacttta ctctttacgt tgactttatt gacagaatta acattcacat atcttgaatt
      421 taatgtccat gatgttgttt gcgtgagaac cttctcagca ttaatgaatt ttttaacgga
      481 atccttggtt tctttttcga gtccgaattt acaatatcat gcatatagac catttcatga
      541 atctgcgaaa actcaatgtc cattgtatac ctcacatttt taccgtgact cgatgttact
      601 gttcaataat caccttccat caatactaaa attaataccc ctaatgtgcc gataacaaat
      661 atagtcattc tacgtaacgt ctccataagg tgatatttga cattatcaga agctgcgaat
      721 tcggattttg ctctaatcaa atgagagaaa tgtaaaatga agccacgaaa tattaataat
      781 agcctatcac tgcaaccatt agttcctgat caggagaaca aaaataagaa aaatgaagag
      841 aaatccgtta atccagttaa aatcacaatg gggtctggtt taaattatat tgaacaagaa
      901 tctcttggag gaaaatatct aatacatgat ttgtcaataa agatagcgga tatttctgaa
      961 gagataattc agcaagcaat attatctgct atgagcatat ataaattttc gataacagat
     1021 gatttaatga gtatggctgt aaatgaactc ataaaactga ccaaaataga gaataatgta
     1081 gacctgaata aattcactac tatatgcaca gacgttctat cccccctcgt caccagacat
     1141 aataaagaaa aaaaacaaac gacattctac ccttcgcaaa aatccccttt ttaattttca
     1201 ttgaaaaaat ggcaattgat actatgaatc caggtcatac acattaaggc atttatgtaa
     1261 tgtcgtaatt a
//
LOCUS       DAZGVQ010000366         1256 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000366 DAZGVQ010000000
VERSION     DAZGVQ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.370"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(45..272)
                     /gene="mchB"
                     /locus_tag="ACHLAG_005372"
     CDS             complement(45..272)
                     /gene="mchB"
                     /locus_tag="ACHLAG_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47"
                     /protein_id="HFP0658416.1"
                     /translation="MREITESQLRYISGAGGAPATSANAAGAAAIVGALAGIPGGPLG
                     VVVGAVSAGLTTAIGSTVGSGSASSSAGGGS"
     gene            complement(300..509)
                     /gene="mchI"
                     /locus_tag="ACHLAG_005373"
     CDS             complement(300..509)
                     /gene="mchI"
                     /locus_tag="ACHLAG_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin H47 immunity protein MchI"
                     /protein_id="HFP0658417.1"
                     /translation="MSYKKLSQLTAIFSLPITILLVSLSSLRIVGEGNSYVDVFLSFI
                     IFLGFIELIHGIRRILVWSGWKNGS"
     gene            618..854
                     /locus_tag="ACHLAG_005374"
     CDS             618..854
                     /locus_tag="ACHLAG_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658418.1"
                     /translation="MIKDNSKVDDSADRLSFTETLFVAHSILPDLTQTKTKENDCYLL
                     FKKKCHIVHIKSRGILRYVTDAIVSQDLMVVAHV"
BASE COUNT          427 a          267 c          228 g          334 t
ORIGIN      
        1 caggtactac cgaaaaagtg cctgtaaaat aaataaaaat ataattagct accgccacca
       61 gcagaagaac tggcactacc acttcccacg gtcgagccaa ttgctgttgt caaaccggca
      121 gatacggctc caactacaac cccaagtgga ccaccaggta ttccggcgag agctccaaca
      181 atagctgcag cacctgcagc attagctgaa gtcgctggcg cacctcccgc cccggaaata
      241 tatcttaact gtgattctgt tatttctcgc atataaactg catataaact ccatcatatt
      301 taacttccgt ttttccagcc tgaccagacc agaatccttc gaatcccatg aatcagctca
      361 ataaaaccaa gaaatattat aaagcttaga aaaacgtcaa cataagaatt cccttcgcca
      421 acaatccgaa gggatgaaag tgaaaccaat aagatagtaa taggtaaact gaatatagcc
      481 gtcaattggg acagtttttt ataactcatt aatccctacc tgttgcttta ttttcattat
      541 gaaaagttac caaatcttag cacctcagtc aggagagtca ggtcatcgat aaagaatatt
      601 ccagtagagt ggcgctcatg atcaaagata acagcaaagt tgatgacagt gctgaccgac
      661 tgtcatttac agaaaccctg tttgtagcac attccatctt acctgacctc acccagacaa
      721 agacaaaaga gaatgattgt tatttacttt tcaaaaaaaa gtgtcatatt gtccatataa
      781 agtcaagagg tattttacgt tatgtcactg atgcaattgt tagtcaggat ctcatggttg
      841 tcgctcatgt ataacctgag acttgaacgc acactaataa cactgacggt catacatcgg
      901 gaaatctgag gaggcataag aatgaattgt gataataatc acagaaatga agaattcatt
      961 gttacctttg ataaaggcaa caagcaagac aattcaagac gaaaacacga taattttcct
     1021 atagaggtag aatcctccgt agagcaggag acacactgta tcacaaataa taagtcggct
     1081 tccggtatag taacacatga ctatgatgcc gattatattt gtggttgtgg tgaaattatg
     1141 tgtcctggtt gcggtcatga cctataacaa acatttttgc aaactgatac ctgatcaggt
     1201 acaagcctgt tgattattat gctgaatatt tcatgttata aaaccatcga tataaa
//
LOCUS       DAZGVQ010000367         1253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000367 DAZGVQ010000000
VERSION     DAZGVQ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.371"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..1025)
                     /locus_tag="ACHLAG_005375"
                     /pseudo
     CDS             complement(1..1025)
                     /locus_tag="ACHLAG_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(1034..>1253)
                     /locus_tag="ACHLAG_005376"
     CDS             complement(1034..>1253)
                     /locus_tag="ACHLAG_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFP0658419.1"
                     /translation="YASPVIAVMLGGEAVLGLLACYASQLNAFAISLTVKSALAFLIL
                     IIYFAPILAERVMPLSFFPEQLQLYIEK"
BASE COUNT          410 a          241 c          203 g          399 t
ORIGIN      
        1 cccagcattc tccacatctt caagccacag cagaagacgt aatatttcat ctatattttc
       61 gaagctaaca taatcgtagc gacgatgggt agcataattt tttcgtgcta attttttatc
      121 tgtgataatt ggtattccga tttcctttgc atatttcctt acgcctaagg ccacctcatt
      181 agttactctt acagaaatca atggaatagg tgaaagatgt ggcttaaagt atatccctat
      241 tgcaatgtga gtagggttgg aaatcatcaa acgactatta ctgacatcag acttcaattg
      301 ctcagaaaga atttcctgat gcgctctctg cgtttagact taatttctgg atttccttct
      361 tgctccttgt attctctttt aacttcttgt ttatccattt tcatatcttt cataaataaa
      421 aaatattcag cgataaaatc aaaaattaag acaattatca tactgccgag acaatacagt
      481 atgagaagga atagcatctc tccccaatct gataataaag atatgatatc tccatcaaga
      541 gttttaaaaa gcagtgattt atgattactc caaaatactt tgatcgccaa tgcaaaaaaa
      601 acaatataaa gaattgcttt aacaaattct tttacggttt ttaagccaaa tatacgcttt
      661 aagccattta ctggattcaa tgaatcaaac ttaaatttaa cagcttcagt tgctaattgc
      721 attttacttt gaaaccagga gacaagtatt gttgtgaaca tgcaaaccag caaaaaagga
      781 atgttcgttt tcaaccaacc aacccccata gctttaaaat agtcccaaat atctggttta
      841 gcgttatgat caaggatgta ttcaagaatc gacataatgt gatgcacatc aaaaacatat
      901 ccgagatata aagtacccac aagcattatt acagagatgg ttaagtctct actttttaga
      961 atttgccctt ttttagaagc atcctggagt ttcttttgtg ttggcttttc agttttattg
     1021 gccatgaaac actctatttc tcaatgtata gttgcaattg ttctgggaaa aaggataacg
     1081 gcattactcg ttcagccagt attggggcaa aataaataat taatattaaa aatgccagcg
     1141 cacttttgac agtcagtgaa atggcaaaag catttagttg tgaagcataa catgccagca
     1201 atcccaggac tgcctcaccg cccagcataa cggcaatcac ggggcttgcg taa
//
LOCUS       DAZGVQ010000368         1249 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000368 DAZGVQ010000000
VERSION     DAZGVQ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.372"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..430)
                     /locus_tag="ACHLAG_005377"
     CDS             complement(<1..430)
                     /locus_tag="ACHLAG_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFP0658420.1"
                     /translation="MNRTSPYYCRRSVLSLLISALIYAPPGMTAFTPDVIGVVNDETV
                     DGSQRVDERGTTNNTHIINHGQQNVHGGVSNGSLIESGGYQDVGSHNNFVGQANNTTI
                     NGGRQTIHDGGISTGTIIDSGNQDVYTGGISNGTTIKGGNS"
     gene            complement(495..614)
                     /locus_tag="ACHLAG_005378"
     CDS             complement(495..614)
                     /locus_tag="ACHLAG_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP0658421.1"
                     /translation="MPHSYAEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(838..996)
                     /locus_tag="ACHLAG_005379"
                     /pseudo
     CDS             complement(838..996)
                     /locus_tag="ACHLAG_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239934.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT          354 a          296 c          252 g          347 t
ORIGIN      
        1 gtgaattacc acccttaatt gtcgttccat tgctgatacc acctgtataa acgtcctgat
       61 tgccactgtc gattatcgta ccggtggaaa tgcccccgtc atgaattgtc tgtcttcctc
      121 cgttaatggt tgtattatta gcctgcccca caaaattgtt atgacttcct acatcttgat
      181 atccacccga ttcaataaga cttccattag ataccccgcc atgaacattc tgctggccat
      241 ggttgataat atgagtgtta tttgttgtac ctcgttcatc tactctttgg ctgccatcta
      301 cagtctcatc gtttaccaca ccaataacat caggagtgaa ggccgtcatc ccaggcgggg
      361 catatatcaa ggcagatatc aataaggaaa gtactgagcg gcgacaataa tagggactgg
      421 tcctgttcat aaatttcatc ctctgaaaag tgaatactga gtagcgttta agcgacctta
      481 gctttgctgc aacatcagcc cacaggcacc agaccagggg attcatcctg aagagacagc
      541 gcaagtgtat tgtgttcacc gctcatcaaa gacatcatga tgaaatgatg atattccgca
      601 taagaatgag gcatttttta aacgcagtgc gctgaagtgt ggttggataa aaaagtcaat
      661 ccattcagga aatacgggcg tattcttttc tttcgacaat gaggccgttg gcaaaataaa
      721 atgatttaca taatcgtttc tgataaatat cttctgctca cataaaaatc acacaataac
      781 tttgagatcg cagattgttt tacttttaca gcattcgtcc ccccattgtt gggcaaatat
      841 agattgggcc agagcacgaa agttaatacc acgttcgcac agctcctcca acagcacgac
      901 aagatgccac atactgcgcc ccagtcggtt cagtttacag actagcagtg cgttccctgc
      961 cgatgatgtc ctgaccagtt ttttcagtcc ggacctcctg ccacggtttt acgttgaagt
     1021 ggtaaccctg agcacgcagt tcttcagtca ggcgtggtgc accgtaacgc tgttattgct
     1081 gggtaagatc aaaaaacttt caggcagcta aggaaagttg aaccagacat tagatgaaat
     1141 atttcaacca attacagcac caattcagtc actgccagcc caccaaataa atcaaggggt
     1201 tacatgaaaa cgtagcccct ttttctttgg tagtgacact aaaatggat
//
LOCUS       DAZGVQ010000369         1246 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000369 DAZGVQ010000000
VERSION     DAZGVQ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.373"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            591..1246
                     /locus_tag="ACHLAG_005380"
                     /pseudo
     CDS             591..1246
                     /locus_tag="ACHLAG_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          431 a          205 c          237 g          373 t
ORIGIN      
        1 gacattgact tttcgatatt aaaatatgac tactttgatg taaatgagta taaaaaaaca
       61 ttacttatga cacatctttt attgacaaag atgcagaata tattgaatta ctagcgaaga
      121 ttgatttttc ttatgaagtt gatttcggca catcaacaac atcgattgaa agcaagtatc
      181 ttccatctct aactgatttc atcgctcaat cactatcaca acaattaaat tgtgacataa
      241 taaccagctt taaaccatta agtggagatg agttttgccc aataacatat ttttcaaaag
      301 gtaaagaagt tatcaatttt acgacattta attcaaattg ttgggatggg gtatgctggg
      361 taaaaaaaag gaggcaaaca aatatccgct aacgtagttt ttcaatagct acaccatgtc
      421 acgtaacatg atgcctggat attaaccagg gaaagatcat cagaagtaca ctttttgtac
      481 taaataattc acattttatg tttaaaaatt gagatattcc tcattacctg aagctgtttt
      541 ttattgctta tacatgatca aatactcctt acataattaa ggagaacaaa atggaactta
      601 aaaaattgat ggaacatatt tctattatcc ccgattacag acaagcctgg aaagtggaac
      661 ataaattatc ggatattcta ctgttgacta tttgtgccgt tatttctggt gcagaaggct
      721 gggaagatat agaggatttt ggggaaacac atcccgatgt tttgaagtga tatggtgatt
      781 ttgaaaatgg tattcctgtt cacgatatca ttgccagagt tgtatcctgt atcagtcctg
      841 caaaatttca cgagtgcttt attaactgga tgcgtgactg ccattcttca gatgataaag
      901 acgtcattgc aattgatgaa aaaacgctcc ggcactctta tgacaagagt cgtcgcaggg
      961 gagcgattca tgtcattagt gcgttctcaa caatgcatag tctggtcctc ggacagatca
     1021 agacggatga gaaatccaat gagattacag ccattcctga acttcttaac atgatggata
     1081 ttaaaggaaa aatcatcaca actgatgcga tgggctgcca gaaagatatt gcagaaaaga
     1141 tacaaaaaca gggaggtgat tatttattcg cggtaaaagg aaaccagggg cggctaaata
     1201 aagcctttga ggaaaaattt ccgctgaaag aattaaataa tccaga
//
LOCUS       DAZGVQ010000370         1241 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000370 DAZGVQ010000000
VERSION     DAZGVQ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1241
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.374"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1241
                     /locus_tag="ACHLAG_005381"
     rRNA            <1..>1241
                     /locus_tag="ACHLAG_005381"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          312 a          287 c          392 g          250 t
ORIGIN      
        1 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
       61 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      121 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      181 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      241 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      301 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      361 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      421 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      481 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      541 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      601 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      661 tttaattcga tgcaacgcga agaaccttac ctggtcttga catccacgga agttttcaga
      721 gatgagaatg tgccttcggg aaccgtgaga caggtgctgc atggctgtcg tcagctcgtg
      781 ttgtgaaatg ttgggttaag tcccgcaacg agcgcaaccc ttatcctttg ttgccagcgg
      841 tccggccggg aactcaaagg agactgccag tgataaactg gaggaaggtg gggatgacgt
      901 caagtcatca tggcccttac gaccagggct acacacgtgc tacaatggcg catacaaaga
      961 gaagcgacct cgcgagagca agcggacctc ataaagtgcg tcgtagtccg gattggagtc
     1021 tgcaactcga ctccatgaag tcggaatcgc tagtaatcgt ggatcagaat gccacggtga
     1081 atacgttccc gggccttgta cacaccgccc gtcacaccat gggagtgggt tgcaaaagaa
     1141 gtaggtagct taaccttcgg gagggcgctt accactttgt gattcatgac tggggtgaag
     1201 tcgtaacaag gtaaccgtag gggaacctgc ggttggatca c
//
LOCUS       DAZGVQ010000371         1228 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000371 DAZGVQ010000000
VERSION     DAZGVQ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.375"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..831)
                     /locus_tag="ACHLAG_005382"
     CDS             complement(<1..831)
                     /locus_tag="ACHLAG_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFP0658422.1"
                     /translation="MAALPYMQLYIADYLADTMHLSAEEHGAYLLLMFNYWQTGKPIP
                     KNRLAKISRLTNERWADVEPSLREFFCDNGDEWVHLRIEEDLASVREKLTKKSAAGKA
                     SVQARRSRKEAYVQTKQERDLTGVQTNVGVVFEHDANTKATNKDTDLKELNPTHNVRE
                     RESIPTSESHGAPLQTAEPEYLDGLSEPIGKFSMTTVWQPSPDFRQRAAVWGMALPEP
                     EFTPAELAAFRDYWMAEGKVFTQVQWEQKFARHVQHVRAQAKPVSKGVSHAASGGTAS
                     R"
     gene            complement(918..>1228)
                     /locus_tag="ACHLAG_005383"
     CDS             complement(918..>1228)
                     /locus_tag="ACHLAG_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP0658423.1"
                     /translation="MHDTSTTEGIGLNIQNIFRWLEKDTPDAVEKIQALIPAILTVLP
                     RELRYHLSIFDTVERRALLAAQEALSTAIDAHDDAVQAVYRKAYFSDGGSSGESVVVH
                     "
BASE COUNT          280 a          331 c          274 g          343 t
ORIGIN      
        1 ccgtgatgcc gtgccacctg atgctgcatg gcttaccccc ttgctgactg gttttgcctg
       61 tgccctgacg tgctgcacgt ggcgggcaaa tttctgctcc cactgaacct gcgtgaaaac
      121 cttcccctcc gccatccagt aatcccggaa tgcggcaagc tctgcaggtg taaattccgg
      181 ctcaggcaga gccatacccc acacagctgc ccgttgtcga aaatccggcg acggctgcca
      241 gacagtggtc atcgaaaatt tcccgatcgg ttcgctcagg ccgtccaggt attcaggttc
      301 ggctgtctgc aacggcgcac catgcgactc actggtagga atactctcgc gttcacgcac
      361 gttatgtgtg gggtttaatt cttttagatc tgtatcttta ttagttgctt ttgtgtttgc
      421 gtcatgttca aacacaacac caacatttgt ttgaacacct gttaaatctc tctcttgttt
      481 tgtttgaaca tatgcttcct ttctgcttct tctggcctga acagatgctt ttccggcggc
      541 tgattttttg gtcaattttt ccctgactga tgccagatct tcctcaatcc gaagatgcac
      601 ccattcatcg ccgttatcgc aaaaaaactc ccgcaaggat ggttccacat cagcccatcg
      661 ctcgttagtc agacgggaaa tttttgccag cctgttttta ggtattggtt tccctgtttg
      721 ccagtaattg aacatcagca acaaatacgc accgtgctcc tctgcggaca aatgcatggt
      781 gtcagccagg taatcagcta tgtacagttg catgtatggt aatgcggcca taattgcctc
      841 atcttgtgac gaaccatcct ctggtgatat tctgtgattc cccaatcaac agaatcagca
      901 ggggtatggc ataaatatca atgcaccaca acagactcgc cggatgaccc gccgtcgctg
      961 aaatacgctt tccggtaaac tgcctggact gcatcatcat gcgcatcaat tgccgtgctc
     1021 aacgcttcct gggccgccag taatgcacgg cgctcaacag tgtcaaaaat actgaggtga
     1081 taacgcagtt cacgcggaag aacagtcagg atagctggaa ttagtgcctg aattttttca
     1141 acagcatcag gcgtatcctt ttcaagccag cgaaaaatat tttgtatgtt caatccaata
     1201 ccctctgtcg tagaggtgtc atgcattg
//
LOCUS       DAZGVQ010000372         1217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000372 DAZGVQ010000000
VERSION     DAZGVQ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.376"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            53..>1217
                     /locus_tag="ACHLAG_005384"
     CDS             join(53..311,311..>1217)
                     /locus_tag="ACHLAG_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP0658424.1"
                     /translation="MNKKTKRTFTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLES
                     WVRQLRRERQGITPSATPITPDQQRIRELEKQVRRLEEQNTIFKKGYRALNVRLAERF
                     TIAARLSDSHTVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSEIRRAWNQSRGSA
                     GARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTSLPNLLERQF
                     AVPEPDRVWCGDITYLWAGNRWCYLAVVMDLFARRVIGWSLSAHADTALISSALRMAY
                     ETRGQPRDVVFHSDQGSQYTGLKYQQLLWRCRINQSVSRRGNCWDNSPMERFFRSLKT
                     EWVPTNGYAGKDEARQQINDYILNYYNSVRPHHYNGGLTPEESENRYHFYCKTVA"
BASE COUNT          328 a          314 c          330 g          245 t
ORIGIN      
        1 ggccacctga tgaaaggtga tattctcacc acaacacaaa acaggtgact taatgaacaa
       61 gaaaaccaaa cgaaccttca cccctgagtt caggctggaa tgtgcacagc tgattgttga
      121 taagggctac tcatatcgac aggccagtga agcgatgaat gtcggttcaa ccacgcttga
      181 gagctgggta cgccagctca ggcgagagcg ccagggtatt acgccctctg ccacacccat
      241 tactccagac cagcaacgta tccgcgagct ggaaaagcaa gttcgccgtc tggaggaaca
      301 aaatacgata ttaaaaaagg ctaccgcgct cttaatgtcc gactcgctga acggttcacg
      361 atagccgcca gactaagtga cagccacacg gttgtcagcc tgtgttctgc tctggaaata
      421 caccgcagca gttaccggta ctggcgaaaa cgacgcgata cggttaatcc ggcacgagtc
      481 aggttgtgca gcgaaatacg ccgggcgtgg aaccaaagtc ggggctctgc gggcgcgcgc
      541 actctggctg aaatgctgac tcaaaacggc gtcccgatga gccgttaccg tgccggacgt
      601 ctgatgaaat atctgaacct gagcagttgt cagcccggaa aacatcagta taaaaatgcc
      661 cgtcaggagc ataccagcct gccgaatctg cttgagcgtc agttcgcagt accggagcca
      721 gaccgggtat ggtgtggaga tattacgtat ctctgggcag gaaatcgctg gtgctatctg
      781 gcggttgtta tggatctttt tgcccgcagg gttatcggct ggagtctgtc agcgcatgcc
      841 gataccgcac tgataagcag tgccctgcgg atggcctatg agacgcgtgg ccaaccccgg
      901 gatgtcgtgt tccatagcga ccagggaagc cagtatacag gccttaaata tcaacaactt
      961 ctctggcgtt gcagaataaa tcaaagcgtc agtcggcggg gaaactgctg ggataacagc
     1021 cccatggaac gcttcttccg cagtctgaaa acagaatggg tgccaacgaa tggttacgca
     1081 ggcaaggacg aggcccggca gcaaattaat gattacatat tgaactacta caacagcgtc
     1141 agacctcacc attataacgg tgggctgacg ccggaagagt cagagaacag ataccatttt
     1201 tactgtaaaa ccgtggc
//
LOCUS       DAZGVQ010000373         1212 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000373 DAZGVQ010000000
VERSION     DAZGVQ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.377"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..423)
                     /locus_tag="ACHLAG_005385"
     CDS             complement(<1..423)
                     /locus_tag="ACHLAG_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0658425.1"
                     /translation="MGKGGGKGHTPVEAKDNLKSTQMMSVIDAIGEGPVEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETVLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQL"
     gene            complement(658..>1212)
                     /locus_tag="ACHLAG_005386"
     CDS             complement(658..>1212)
                     /locus_tag="ACHLAG_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP0658426.1"
                     /translation="ALSLQVPGFRRQMNEGWYQIRIAGYDTAPEAVYARLHEQLGEGT
                     VIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGTALSAGGFSATTMLFSLG
                     ASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSR
                     RISQDISTRDEGGGGKVVVIGRQG"
BASE COUNT          250 a          388 c          290 g          284 t
ORIGIN      
        1 taactgaatc agcagacgga cagaggacgg attacggtca ccctttgacg tggtctccac
       61 cagtgactgc accccgaagg tcacccgcag gcggtcaatg ttcgctgacg taatggtgcg
      121 tgtcaccggt tttgccttcg tcacttccac acccagtaca gtttcagctc cggaggactc
      181 aaagccttcc ggtggtgtct gctcctgctc cccggcgcgc cagaccgcgg tcacaccgtg
      241 tatcacggga ttaccgtccg tgtccgtcag cggggttttg ttcaccagga tactctgcag
      301 tcccttcacc gggccttcca ccggtccctc tccaatcgca tcaatcacgc tcatcatctg
      361 cgtggatttg agattatcct ttgcctcaac cggcgtgtgc cccttgccgc cacctttgcc
      421 cattgtctca ccctttactg tgataactgt tacgcacaaa aacaacaggc atcccggagg
      481 atgcctgtat catgactgaa taaaaattct gaatttcttc acattctgaa aaaaaagact
      541 gttgcgctga taaatacgct gcgttacttt ttagtgcctg aaaaataact ccataacgtt
      601 aatcttcatc gttctctccc gcagctccgc gatcactgcg ggattttttt attcttttta
      661 cccctgccgc ccgataacca cgacctttcc gcccccgcct tcatcacggg tgctgatgtc
      721 ctgggatata cggcgggagc caaccagcat ttccccgtaa ggcaccggca tcgggttccc
      781 ctgggcaatc atgttatcca gcgaggaaaa gtacgtgttc tgtctgccgt tatccgttgc
      841 gcggtaatcc ggtgtttttg ccttcggggc cagcatctgg gccacaccgc ccagaatcat
      901 gctggctccc agtgaaaaca gcatcgtggt ggcagaaaaa ccgccggcac tcagggctgt
      961 accccataac gccatcgagg caccggcagt gaagaaagag cccacgatgg ctgccgcccc
     1021 cagcacaatc tgcagtccac cctttccggc cccggccagt cgcggcacaa tgtggatgac
     1081 cgttccctca cccagctgtt cgtgaagacg ggcgtacacc gcctccggtg ccgtgtcata
     1141 accggcaata cgtatctggt accagccttc gttcatctga cggcggaatc cgggcacctg
     1201 taacgacagc gc
//
LOCUS       DAZGVQ010000374         1211 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000374 DAZGVQ010000000
VERSION     DAZGVQ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.378"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..535
                     /locus_tag="ACHLAG_005387"
     CDS             <1..535
                     /locus_tag="ACHLAG_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658427.1"
                     /translation="GFRRQMNEGWYQIRIAGEDTAPEAVYARLHEQLGEGTVIHIVPR
                     LAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGSALAAGGFSATTMLFSLGASMILGG
                     VAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDIS
                     TRDEGGDGKVVVIGRQG"
     gene            789..>1211
                     /locus_tag="ACHLAG_005388"
     CDS             789..>1211
                     /locus_tag="ACHLAG_005388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0658428.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPVEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQL"
BASE COUNT          279 a          296 c          396 g          240 t
ORIGIN      
        1 gggctttcgc cgtcagatga acgaaggctg gtaccagata cgtattgccg gtgaggacac
       61 ggcaccggag gcggtgtatg cccgccttca cgaacagctg ggtgagggaa cggtcatcca
      121 cattgtgccg cgactggccg gtgccggaaa gggcggactg cagattgtgc tgggggcggc
      181 ggccatcgtg gggtcgttct tcacggccgg agccacgatg gcgttatggg gttcagccct
      241 ggcagccggt ggtttttctg ccaccacgat gctgttttca ctgggtgcca gcatgattct
      301 gggcggtgtg gcccagatgc tggccccgaa ggcaaaaaca ccggattacc gcgcaacgga
      361 taacggcaga cagaacacgt acttttcctc gctggataac atgattgccc agggtaaccc
      421 gatgccggtg ccttacggtg aaatgctggt tggttcacga cggatatccc aggacatcag
      481 cacccgtgat gaaggcggtg acgggaaagt ggtggttatc ggacggcagg ggtaaaaaga
      541 ataaaaaaat cccgcagtga tcgcggagct gcgggagatt cagacaaatg aagattagtg
      601 ttaaggagtt gttttttatg tcacggcaaa aaaacactaa cgcagcgaaa ttatcagcgc
      661 cacagtcagt ttgtgaaaat gtgaagatat tcagaatttt tattccgtca tgatgcaggc
      721 actctccggg gtgcctgttg tttttataca tcaacagtgt ccggcagcgg acgatgaagg
      781 gggattccgt gggcaaaggc ggcggcaagg cgcacacacc gcgtgaggcg aaggataatc
      841 tcaaatccac gcagatgatg agcgtgattg atgccatcgg tgagggaccg gtggaaggcc
      901 cggtgaaggg actgcagagt attctggtga acaaaacccc gctgacggac acggacggta
      961 atcccgtgat acacggtgtg accgccgtct ggcgtgccgg ggagcaggag cagacaccgc
     1021 cggaaggctt tgagtcctcc ggggcggaaa ccgcactggg cgtggaggtg acgaaggcaa
     1081 agccggtgac gcgcaccatc acgtcagcga acattgaccg tctgcgggtc accttcgggg
     1141 tgcagtcact ggtggagacc acgtcaaagg gtgaccgtaa tccgtcctct gtccgtctgc
     1201 tgattcagct t
//
LOCUS       DAZGVQ010000375         1206 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000375 DAZGVQ010000000
VERSION     DAZGVQ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1206
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.379"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            526..1077
                     /gene="ybcL"
                     /locus_tag="ACHLAG_005389"
     CDS             526..1077
                     /gene="ybcL"
                     /locus_tag="ACHLAG_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFP0658429.1"
                     /translation="MKTLIVSTVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGVPEGTKSFAVTVYDPDAPTGSGWWHWTVVNIPATITYLPVDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTEKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            1087..>1206
                     /locus_tag="ACHLAG_005390"
     CDS             1087..>1206
                     /locus_tag="ACHLAG_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP0658430.1"
                     /translation="MLARGKTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKK"
BASE COUNT          380 a          190 c          271 g          365 t
ORIGIN      
        1 atccgcccag caggcgataa gtgctctcaa atatatggtt gatggtgaga tttatttcta
       61 aataatgatc tcggatttta agttatgcta tggtgataaa gtgcaagaca gaattaatta
      121 tctttgacga aacttaatgg gtaattactt tgtttgctcc cacaagcgag ttttgtacgg
      181 ctgtattggg gtagtaaatg agctatacaa tcttaatcat ttgttaggtg agaactcttg
      241 gtcgcagatt caaatactga aaatacgtga caaattatta tgagcaaaat ggtgtatgtc
      301 acgtattttg aatgataggt taaaaaataa caccgacttt cgtaggtatt actaataata
      361 aagcagagtt tttagatagt atcaatgtgc tttgtgtata ttgtggcaaa taattgggtt
      421 gggggtacaa ttgtgattgc ttttgcataa aaattgcgcc tttatgcata atgagataaa
      481 ggaatatcaa ataaaataac gataggtcat aacaaagagg tttttatgaa aacacttatc
      541 gtttcaactg tattggcatt cataacattt tctgcgcagg ctgcagcatt tcaggtcact
      601 agtaatgaaa taaaaacagg agagcaactt acaacgtctc atgtcttttc tggatttggg
      661 tgtgaaggtg gtaatacatc gccctcatta acctggtctg gtgttcctga aggtaccaaa
      721 agctttgccg taactgtata tgatccagat gcacctacag gcagtggttg gtggcattgg
      781 actgttgtta atattccagc aacaataaca tatttgcccg ttgatgcagg gagacgtgat
      841 ggaacaaaac tgccgactgg tgctgttcaa ggccgaaatg attttggcta tgctgggttt
      901 ggtggcgcat gtcctcctaa aggagataaa ccacatcatt accagtttaa agtatgggct
      961 ctaaaaactg aaaagattcc tgtagattct aactccagcg gagcgttagt tggttatatg
     1021 cttaatgcta ataaaatcgc aaccgctgaa ataacaccag tttatgagat aaagtagggt
     1081 gagagtatgc tggcaagagg taagactaac ttaaagatcg aagaaatacg gatgcataaa
     1141 catcatgaga ttcatagggt taagcctctt atgccagctt tgtgtcgtat ccgtcaggga
     1201 aagaaa
//
LOCUS       DAZGVQ010000376         1198 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000376 DAZGVQ010000000
VERSION     DAZGVQ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.380"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..154
                     /locus_tag="ACHLAG_005391"
     CDS             <1..154
                     /locus_tag="ACHLAG_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFP0658431.1"
                     /translation="NNYVDEYYKQWGKQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQG
                     SNWTFQ"
     gene            171..411
                     /locus_tag="ACHLAG_005392"
                     /pseudo
     CDS             171..411
                     /locus_tag="ACHLAG_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            431..762
                     /gene="sctI"
                     /locus_tag="ACHLAG_005393"
                     /pseudo
     CDS             431..762
                     /gene="sctI"
                     /locus_tag="ACHLAG_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            759..1198
                     /gene="sctJ"
                     /locus_tag="ACHLAG_005394"
                     /pseudo
     CDS             759..1198
                     /gene="sctJ"
                     /locus_tag="ACHLAG_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     lipoprotein SctJ"
BASE COUNT          399 a          203 c          246 g          350 t
ORIGIN      
        1 taataactat gttgacgaat attataaaca atggggtaaa caatatgtaa gatttaatgt
       61 gaatttgaaa aatcaggaca caaataatag ttcatttagc tacggagata accgattcga
      121 gaagtctcaa ggtagcaact ggacatttca gtaataattg aggaggactt atggcagatt
      181 ggaatggtta tattatggat atcagcaatc aatttgatca gggcgttgat gatctgaacc
      241 agcaagttga aaaagcgttg gaggatttag ccaaccaatc cctccgacac gaaatttctt
      301 gccgaatatc aaagtgcatt agctgagtat acattatatc gaaatgcgca atctaacgtt
      361 gtaaaagcgt ataaagatct tgattcagca atcatacaaa acttccgcta aactggataa
      421 ggattttatt atgtcagtaa gcaatatgcc acctattgat agagctgagc aatcaactgc
      481 tcatgaaata cagcaagcga aagtgattga tttaaatgac cgggttctca atcttgacaa
      541 cccggatgat aaaatgatct ccgcttttgc caactatgct gtacaaactg aaaattggca
      601 gcaaaatgcg ttacaagcat taaggtctga taaagagggg ttaactcctg aaaaattatt
      661 agtcttgcag gatcatgtgc ttaattataa tgtcgaggtt tcattggtgg aacattagca
      721 agaaagattg tcgctgcagt tgagacgttg acccgctcat gaaatatata tcattattat
      781 tgtttatcct gctgttgtgc ggctgcaagc agcaggaatt attgaaccat cttgatcaac
      841 agcaagctaa tgatgtcctt gctgttttac aaagacataa tattaatgct gaaaagaagg
      901 attaaggcaa aacaggattt tcaatatttg tcgaaccaac agattttgct tctgcggtgg
      961 attggctaaa aatctacaat ctaccgggta agcctgatat tcaaatatcg cagatgttcc
     1021 ctgcagatgc actcgtttca tctccacgag cggaaaaagc aaggctatat tctgctattg
     1081 agcagcggtt agagcaatct ctcaaaatta tggatggcat tatttcttcg agagtacatg
     1141 ttagttacga tgttgataat ggcgatagtg gtaaaactgc tttaccaatt catatttc
//
LOCUS       DAZGVQ010000377         1174 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000377 DAZGVQ010000000
VERSION     DAZGVQ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.381"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            100..1074
                     /gene="cysB"
                     /locus_tag="ACHLAG_005395"
     CDS             100..1074
                     /gene="cysB"
                     /locus_tag="ACHLAG_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFP0658432.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT          303 a          277 c          272 g          322 t
ORIGIN      
        1 gttatagtta gcaccttttt tattattaaa tcgtattagt cacccgccag gtgtgacgaa
       61 aaaacgatgt tctgatggcg tctaagtgga tggtttaaca tgaaattaca acaacttcgc
      121 tatattgttg aggtggtcaa tcataacctg aatgtctcat caacagcgga aggactttac
      181 acatcacaac ccgggatcag taaacaagtc agaatgctgg aagacgagct aggcattcaa
      241 attttttccc gaagcggcaa gcacctgacg caggtaacgc cagcaggaca agaaataatt
      301 cgtatcgctc gcgaagtcct gtcgaaagtc gatgccataa aatcggttgc cggagagcac
      361 acctggccgg ataaaggctc gctgtatatc gccaccacgc atacccaggc acgctacgca
      421 ttaccaaacg tcatcaaagg ctttattgag cgttatcctc gcgtttcttt gcatatgcac
      481 cagggctcgc cgacacaaat tgctgatgcc gtctctaaag gcaatgccga tttcgctatc
      541 gccacagaag cgctgcatct gtatgaagat ttagtgatgt taccgtgcta ccactggaat
      601 cgggctattg tagtcactcc ggatcacccg ctggcaggca aaaaagccat taccattgaa
      661 gaactggcgc aatatccgtt ggtgacatat accttcggct ttaccggacg ttcagaactg
      721 gatactgcct ttaaccgcgc agggttaacg ccgcgtatcg ttttcacggc aacggatgct
      781 gacgtcatta aaacttacgt ccggttaggg ctgggggtag gggtcattgc cagcatggcg
      841 gtggatccgg tcgccgatcc cgaccttgtg cgcgttgatg ctcacgatat cttcagccac
      901 agtacaacca aaattggttt tcgccgtagt acttttttgc gcagttatat gtatgatttc
      961 attcagcgtt ttgcaccgca tttaacgcgt gatgtcgttg atgcggctgt cgcattgcgc
     1021 tccaatgaag aaattgaggt catgtttaaa gatataaaac tgccggaaaa ataatttctt
     1081 gcgttatttt cggcaccttt tatgtagcga aggtgccgga atatattctc ttttgttact
     1141 tatttgtata ccgtgcacaa tatttttatc cctc
//
LOCUS       DAZGVQ010000378         1169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000378 DAZGVQ010000000
VERSION     DAZGVQ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.382"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            122..724
                     /gene="ais"
                     /locus_tag="ACHLAG_005396"
     CDS             122..724
                     /gene="ais"
                     /locus_tag="ACHLAG_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFP0658433.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(763..>1169)
                     /gene="nudI"
                     /locus_tag="ACHLAG_005397"
     CDS             complement(763..>1169)
                     /gene="nudI"
                     /locus_tag="ACHLAG_005397"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFP0658434.1"
                     /translation="CPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGERIEEALR
                     REIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSANREVKINE
                     EFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          313 a          268 c          249 g          339 t
ORIGIN      
        1 ttgaggcatt ttgccgacat cgtaggattt ttaatattac accaactgct aattatcgcc
       61 agaaatgtcg ctcaatttta cggtaattgt ctggttgcgc ttgtctatag gtggagttta
      121 cgtgttagct ttttgccgct catcattgaa gtcaaaaaaa tatttcatca ttttactggc
      181 gctcgctgca attgccggac tgggtactca tgccgcctgg agtagcaatg gtttgccacg
      241 tatcgacaat aaaacattgg ccagactggc acagcagcac ccggttgtcg ttttgtttcg
      301 ccatgctgaa cgttgcgatc gttcaaccaa tcaatgcttg tcagataaaa caggtattac
      361 ggttaaaggt acccaggatg cccgtgaact gggcaacgct tttagcgctg acatccctga
      421 tttcgatctt tattccagta ataccgtccg gaccattcag tcggcgacct ggttttccgc
      481 gggtaaaaag ctgacggtag ataaacgact tcttcagtgc ggtaatgaga tttatagtgc
      541 aattaaggac ttacaaagca aagcgcctga taaaaatatc gttattttca cccataatca
      601 ttgcctgaca tatattgcta aaaataagcg tgatgcgaca tttaaacctg attatctgga
      661 tggtttagtc atgcatgtgg aaaaaggcaa agtttatctg gatggggaat tcgttaacca
      721 ctaaattttc aatctgacag ccagtgatgg ctgtcatcat tgttaaagaa gacctttcaa
      781 acgtaacgtt tttcgggtgg caacatttaa atcataatgc accagatctt caggttttac
      841 ccacgcgtag tcctgaaact cttcgtttat tttcacttct cggttggcag aaacgcagtc
      901 aaaaatcagg taaatcatat aaatctcttc cttgcgacca tctgcatacg tcttggtgcg
      961 aatatcatcg ctgaaggtcc acggcgtgat ttctgtcaaa agcagctgtt ctcccagttc
     1021 ttcgcgaatt tcgcggcgta gtgcctcttc aattcgttcg ccagactcca cgccgccacc
     1081 cgaaagcgcc cattgaccgg ggaaaacgcc gcgatcgtcg gccattttac acagcaaata
     1141 agcaccatca ttttgaatca aagggcata
//
LOCUS       DAZGVQ010000379         1166 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000379 DAZGVQ010000000
VERSION     DAZGVQ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.383"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..223)
                     /locus_tag="ACHLAG_005398"
     CDS             complement(<1..223)
                     /locus_tag="ACHLAG_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658435.1"
                     /translation="MIQYLIKSKVDRIQCNDTGKRIYETLAYLYKGKPTPLKYSDVLH
                     RAACSEDGLKFWLKQLSNFGVIEIKELSFS"
     gene            complement(240..911)
                     /locus_tag="ACHLAG_005399"
     CDS             complement(240..911)
                     /locus_tag="ACHLAG_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP0658436.1"
                     /translation="MIKHTKPFLGIDYDLLAITSLVINGKTKEVKLHHKIIYSYLLSL
                     SGTYQKVTPSLKGIGERLGVGTDQTVRRHIKDLVSFGWLKIEERAGSTNAYIVLPYEQ
                     EAKTQQEATVKAVPEQLPPTEDNKESSFDVLDDWDAPLPWETEETPVSPIFDEKVTND
                     REDVLENFASLICQEKNTRTGGASFIEFANSLAYRYGLKLPNGIEAYFAKKHPKVYED
                     FDLPF"
BASE COUNT          321 a          236 c          198 g          411 t
ORIGIN      
        1 tagagaaaga taattcttta atctctataa caccgaaatt tgatagctgt ttcagccaga
       61 attttaaacc atcttcagag caagctgctc gatgtaacac atcgctatat tttagcggtg
      121 ttggctttcc tttatagagg taagcaagcg tttcgtagat gcgttttcct gtgtcattac
      181 actgaattct gtctactttg ctttttatta aatactgtat caatagtcct ccttacttat
      241 taaaagggaa gatcaaagtc ttcatatact ttgggatgtt tcttagcaaa atatgcctca
      301 ataccatttg gtagctttag cccgtatcta taagctagtg aatttgcaaa ctcaataaat
      361 gatgcccctc ctgttctggt gtttttctcc tggcagataa gagaggcaaa attttctaac
      421 acatcctccc tatcattcgt taccttttca tcaaatattg gtgaaacagg tgtttcttcc
      481 gtctcccacg gcaaaggcgc atcccaatca tcaagaacat caaaagaaga ctctttatta
      541 tcttctgtag ggggaagctg ttcaggaaca gctttaacgg tagcttcttg ttgtgttttg
      601 gcttcttgtt cataaggaag gacaatataa gcatttgtgc tacctgccct ttcctcaatc
      661 ttcaaccaac caaaagaaac aagatctttg atatgacggc gtaccgtctg atctgtacct
      721 actcctaacc gctcaccaat acctttcaaa gatggtgtta ccttttggta tgtgccagat
      781 aaacttaaca aataagaata aatgatctta tggtgtagtt ttacttcttt tgttttgccg
      841 ttaatcacca gggaagtgat tgctagtaag tcataatcta ttcctaaaaa aggttttgta
      901 tgtttaatca ttaaaaatct ccttgatgta atcacgctct atggcgctac tatggtgata
      961 aaaatcactt gcttttattt gagagtgttt cagcctctct tttcaacata tcaagaatgt
     1021 aatctgtgac agtgatccgt ctacttctgg cttctttaaa taacacctga aatacttcat
     1081 caggaatatg aaagcaagtg cgtggttgtc ttttctgtag tttcgctcat agttttctcc
     1141 ttctcttctt tactctgttt cagatt
//
LOCUS       DAZGVQ010000380         1158 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000380 DAZGVQ010000000
VERSION     DAZGVQ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.384"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(119..1156)
                     /gene="ompC"
                     /locus_tag="ACHLAG_005400"
     CDS             complement(119..1156)
                     /gene="ompC"
                     /locus_tag="ACHLAG_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP0658437.1"
                     /translation="MMAMSAQAAEIYNKDSNKLDLYGKVNAKHYFSSNDADDGDTTYA
                     RLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKFGDYGSIDYGR
                     NYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGLVDGLNFAAQY
                     QGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQVIYGNNSLNASGQNA
                     EVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEVVAQYQFDFGLRPSVA
                     YLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINLIDKSDFTKASGVATD
                     DIVAVGMVYQF"
BASE COUNT          324 a          257 c          251 g          326 t
ORIGIN      
        1 ataacagaag gtttatatag ttagaagcaa ggttgtgctc ctaaaggaag tggcttgagg
       61 gagccactta tatgttgggg aggcaaagcc tcccgcaaca tatcttttag taatcaaatt
      121 agaactggta aaccatacct acagcaacga tatcatcggt agcaacgcca gatgctttcg
      181 tgaaatcgct cttatcaatc aggttgattt tgtaatcaac aaaagtggac atatttttgt
      241 tgaagtaata ggttgcacct acatcaatat attcaaccag gtcctgatca ccccacgcac
      301 ccaagtcttt tcctttagat tgcaggtaag caacggatgg acgcagaccg aagtcgaact
      361 gatattgtgc aactacttcg aagttttgtg ctttgttggc aatatggtta ttaccaaaaa
      421 cagtcatgtt ctgggtttca gaataggtgg tagccagata gatgttgttc gcatcatatt
      481 tcagaccagc tgcccatact tcagcatttt gaccagatgc attcaggctg ttgttaccgt
      541 agataacctg attattagtg cggtcagatt tagcataggt tgcacctaca ccgaatcctt
      601 catactcata agtagtggag aaaccgaaac catcaccatt agcttcagtt acgtcagtgc
      661 ggtcattttt accctgatac tgagcagcaa agttcagacc atcaaccaga ccaaagaagt
      721 cgttgttacg ataagttgca acaccagtgg tgcgaccagt catgaacaca tctgtttggg
      781 tccaggtatc gccaccgaat tctggcagaa cgtcagtcca cgcaccgatg tcatatgcta
      841 caccgtagtt acggccgtaa tcgattgagc cgtagtcccc gaatttcagg cctgcaaatg
      901 caagacgggt tttgtctttg gaggagcctt gagattcagc gcggttgcct ttgaattcat
      961 attcccactg accgaaacca gtcagttgat cgttgatttg ggtttcacct ttgaagccaa
     1021 gacgggcata agtagtatca ccatcatctg catcattaga ggagaagtag tgcttggcat
     1081 taactttccc atatagatcc agcttgttac tgtctttatt ataaatttca gctgcctgag
     1141 cagacatcgc catcagta
//
LOCUS       DAZGVQ010000381         1152 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000381 DAZGVQ010000000
VERSION     DAZGVQ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.385"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(549..923)
                     /locus_tag="ACHLAG_005401"
     CDS             complement(549..923)
                     /locus_tag="ACHLAG_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658438.1"
                     /translation="MEFKDLPVPFQEMASNVVRSQLATLDLSTVEKETIDNISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLELMDIINKGFGLLMKKKGIRIAPLENHFTASSINS
                     CDLKHHTSDGKVESNNKISINH"
     gene            complement(935..1087)
                     /locus_tag="ACHLAG_005402"
     CDS             complement(935..1087)
                     /locus_tag="ACHLAG_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP0658439.1"
                     /translation="MDTIELGNSESLVCGVFPNQDGTFTAMTYTRSKTFKTEAGARRW
                     LARNTY"
BASE COUNT          298 a          283 c          249 g          322 t
ORIGIN      
        1 ccattatcgc gtcctcaaca atgaattttg tgatgcggtg cctggtgcct ccaggtgacg
       61 ttaaccagtt aacaattaac gccggaatac agaaggatgc ccgttacgcc ccgtaaaaga
      121 ccactttacg gttttaactg ttccgcgtgc gcatagccgc attcaccgca tcacaaaatt
      181 cactttaaaa agggtggcag agcagtcacg gagtaaaact gataccgcca aacgtcacca
      241 gaaaattgat aacagagggc gttgcagctg ggttgtcact taagcgtatg gtcaacctga
      301 caacccggtg tcctcaacgg ggaaggaata accccgccat acttacctcc gcgccatttc
      361 gcggagtgcc acaaccggaa gcgcacggtc gacgaaaatt taacgacgac ctatacagag
      421 agactaactt cgccgggcgc tttcgtgtta tgccctgact tttcagggat atatcctttt
      481 cagttaactg tcagtgccgg attcttatcc gtgtccggcg cacgcactct acctcacctg
      541 tgaataaatt aatgattaat tgatattttg ttgtttgatt caactttccc atcggatgtg
      601 tgatgcttta aatcacagga attaatactg cttgctgtaa aatgattttc aaggggagct
      661 attcgaatcc ctttcttttt cattaacaag ccaaatcctt tattaatgat gtccattaat
      721 tccagaaagt atttttcatg taaatcctgg ttatcagaga gctgcttctc ttcgtacagc
      781 ccgataaagg ctcggcgtac gttaccggat atattgtcga ttgtttcttt ttctacggta
      841 ctcaggtcaa gagtcgccag ttgagagcga accacattcg atgccatttc ctggaatggt
      901 actggtaaat ctttaaattc catcgtcaac ctcatcagta agtgtttctg gctaaccagc
      961 gacgcgcgcc agcttcagtt ttaaacgttt tgcttctggt atacgtcatc gcggtaaacg
     1021 tgccgtcctg gttgggaaac acgccgcata ccagagattc gctgttgcca agctcgatag
     1081 tatccatgct gacctcattt ccccttaacg ccgggggagc ggaacaaaaa cctgctgcat
     1141 agttattaaa gt
//
LOCUS       DAZGVQ010000382         1150 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000382 DAZGVQ010000000
VERSION     DAZGVQ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1150
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.386"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            5..>1150
                     /locus_tag="ACHLAG_005403"
     CDS             5..>1150
                     /locus_tag="ACHLAG_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658440.1"
                     /translation="MSKKFTMTLLSSSLGGLLLGISSGVLAQELPPIKDSGLPFYSVL
                     KEADASHVTYGSTYRIYYNKSSGWGTMRYVLNGKDETLFNFDQEGNIIVLSKDNSIAY
                     TVHEPVLKDFARMAAGLRTSDKENGKHQVDEEEVRRIFNKVNNLSNTIINPEILKQSA
                     FIASGPRPEGDRLVQASAAGEFALAVGTGAGADKKFATSVGSWSAASGQQSVALGGGT
                     YAYADASTALGSVAFVDNTARYGTAVGNRAKVDKDATEGTALGAKATVTNKNSVALGA
                     NSRTTRDNEVYIGYEAEPGKAYKTRVLGGLSDGTRPSDAATVRQVDRVKDSVEQLAQD
                     TNTRLVVEAKKSREYTDSRTTVGVNSDGTLTRAEGASKTLAVNDGLVA"
BASE COUNT          329 a          228 c          298 g          295 t
ORIGIN      
        1 cataatgagt aaaaagttta caatgacact tctgtcatct tctctgggtg ggcttctgct
       61 tgggatcagc tcaggtgttt tagctcagga gttacctcct atcaaagatt ctggtttgcc
      121 tttttattca gtgctgaaag aggcagatgc ttcgcatgtt acatacggta gtacttatcg
      181 tatttactat aacaaatcct ctggttgggg aacaatgcga tatgttctga acggaaaaga
      241 tgagaccctt tttaatttcg atcaggaggg taatattata gtattgagta aagataactc
      301 aattgcatat accgttcatg agcctgtgct aaaagacttt gcccgtatgg cagcaggcct
      361 cagaacgtct gacaaagaaa acggtaagca tcaggttgat gaggaggagg tccgccggat
      421 cttcaataaa gttaataatc tatcaaatac tattattaat cctgaaatat taaaacaatc
      481 ggctttcatc gcatcaggac cacgtccgga aggtgaccgt ttggttcagg ctagtgcggc
      541 gggcgaattc gctctggctg ttggtacagg ggccggagcg gataaaaaat ttgcaacatc
      601 agtgggatca tggtctgcag caagtggaca gcaaagtgtg gctttaggtg ggggaacata
      661 tgcttatgca gatgcatcta cagccctggg aagcgttgca tttgttgata acacggctag
      721 atatggcacg gcagtaggta accgagccaa agttgataaa gatgcaacag aaggtactgc
      781 gttaggggca aaggcaacag taacaaataa aaacagtgtt gcattaggag ctaattcacg
      841 cacaacccgt gacaatgagg tttatatcgg ttatgaagca gagccgggta aggcttataa
      901 aacccgggtg cttggtggtc tgagtgacgg tactcgtccc tcagatgctg caaccgttcg
      961 tcaggttgac cgtgtaaaag acagtgttga gcaactggct caggatacga atacccgcct
     1021 tgtggttgaa gcgaaaaagt ctcgtgaata tacagactcc agaacgacag ttggtgttaa
     1081 ctccgacgga accctgaccc gtgcagaagg agccagcaaa accctcgctg ttaatgacgg
     1141 tctagttgct
//
LOCUS       DAZGVQ010000383         1149 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000383 DAZGVQ010000000
VERSION     DAZGVQ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.387"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            37..462
                     /locus_tag="ACHLAG_005404"
     CDS             37..462
                     /locus_tag="ACHLAG_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658441.1"
                     /translation="MTELTKEQLIEEAKLKIAIAKCHPNSGVARVEGELFKIALTSLE
                     AEPVAWTDDEELRDIEKYGFGEIFQRPSDKYEASRCVTPLYRMPPVPVVPEGLVKAVH
                     FYEQVKRENPPVETGAWKDAVDWVLKEVCQVVNIRIKGE"
     gene            464..642
                     /locus_tag="ACHLAG_005405"
                     /pseudo
     CDS             464..642
                     /locus_tag="ACHLAG_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          340 a          221 c          311 g          277 t
ORIGIN      
        1 acatcaggag gccgcccgaa agagcggtaa tgaaaaatga ctgaattaac aaaagagcaa
       61 ttaatcgaag aagccaaatt aaaaatagcg attgcgaaat gccatcccaa ttcaggcgtg
      121 gcacgagtag agggtgagtt attcaaaatt gcactgacat cgctggaagc agaaccggtg
      181 gcatggactg acgacgagga actgcgagat attgaaaaat atgggttcgg tgagatattt
      241 cagcgtccgt cagataaata tgaggcttcg cgttgtgtta ctccattgta tcgcatgccg
      301 ccagtgcctg tagtacctga aggactggtt aaagctgtgc acttctatga acaagtaaaa
      361 cgtgagaatc cgccagttga gactggagcg tggaaagacg ctgttgactg ggtactcaaa
      421 gaggtttgtc aggttgtaaa cattcgcatc aaaggagaat gagatggcgt taacacaccg
      481 cgaactctgt cagattgcgt acaggttcct taagcgcaac gggttcaagg tttgctttca
      541 tgaccgcttt gttgctgtaa ccagtactgg agaacaacca gatgctatgg gattcagaaa
      601 ttcagcatca tgcctgatag aggcgaagtg ttctcgtgct gacttgttgg cagatagaaa
      661 aaagcgtttc cgtaaaaatc cctctcttgg catgggcgac tggagattct ttattagtga
      721 gccgggaatt atttcaattg aggatttacc acctggctgg ggattacttc acgttgttaa
      781 cggaagagta cggaaagtac atgggtggcc caagggtaat tgctgttggg gtgatcctga
      841 cgataagcca tttactggaa ataagcaggt tgaatgcgat tacatgttgt ctgcattaag
      901 gcgcatggag ttgaggggac accttaatga aatatatgac ggtgtaatcg ttaataagca
      961 agaaggaagc gcggcatgac cacttttacc agagagcagt taatagctca cgcagaggag
     1021 actattgaag cacagagact gtgcataccg ggcacaatcg accatgacat catccgcaca
     1081 tataagatgg atattgctgt tctggaaatc gcactggcat cgctggcagc agagccagcc
     1141 ggtaaattg
//
LOCUS       DAZGVQ010000384         1092 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000384 DAZGVQ010000000
VERSION     DAZGVQ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.388"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..474
                     /locus_tag="ACHLAG_005406"
     CDS             <1..474
                     /locus_tag="ACHLAG_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0658442.1"
                     /translation="RGEPGAPGPKGDAGPAGPAGKDGTAGAEGKAGPAGPRGERGPAG
                     AQGVPGPVGPAGPAGKTGPQGPAGERGPMGPPGPAGDAGNGVIKGIRLGNESVFRPHE
                     GYINWDIRLTDGSVLTGVVGMNASGTNRINEIYYRPLQITTDGVYWRTIGIDNGM"
     gene            824..>1092
                     /locus_tag="ACHLAG_005407"
     CDS             824..>1092
                     /locus_tag="ACHLAG_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0658443.1"
                     /translation="MIIKKFTLYSPEKADVPGAMYLKSEDGRDWYESQSLFSADTLKV
                     AYDSNGIICGISKDVSVMWPVNMSVAEVPDSEENRKADSSGRWKF"
BASE COUNT          289 a          220 c          331 g          252 t
ORIGIN      
        1 aggggggaac ctggcgcacc tggtcccaaa ggtgatgcag gcccggcagg ccccgcagga
       61 aaggatggta ccgccggagc ggaaggtaag gcaggtccgg cagggccacg aggtgaaagg
      121 ggacccgcgg gggcacaggg tgttccgggg ccggttggtc cggcaggtcc tgcgggtaaa
      181 acggggccac agggccctgc cggagagcga gggccgatgg gaccgcccgg gccagcaggt
      241 gatgcaggga atggcgttat aaaaggcatt cggctgggaa atgagagcgt gtttcgtcca
      301 catgaaggct atatcaactg ggatatacgc ttgacggatg gcagtgtgct tacgggagtg
      361 gtgggaatga atgcgtcagg gacaaaccgg ataaatgaaa tctattacag gccgttacag
      421 ataacaacag atggcgttta ctggcgtact attggcattg ataatggtat gtgagcagat
      481 tataccatcc ttaccggatg agatataaaa gttaaaagag gaaatgtcgg gttagcatct
      541 ttctgattcg tccggctgat atcaccacgc catatgttta cgctgaacat ggcggataaa
      601 aagacggggg agcaggcagc agactcccct gaaagttatc cctgtcattt ttacattttt
      661 attctgtgtc acggttacac atattttccc gaataaccac tactgatact taccgtattc
      721 tggtgattat ttttgtgatg tgaactgcat tttatttata aagagcaaac agattaacag
      781 ccgcgcgttt tactgtgcag tgctgcgaaa gggtaacgga tttatgatta ttaaaaaatt
      841 cacgctgtat tccccggaga aggcagatgt ccccggggca atgtatctga agtcagaaga
      901 cggcagggac tggtatgaaa gccagtcttt attttcagca gatacgctga aggtggctta
      961 tgacagtaac ggaataatct gcggtataag caaggatgta tctgttatgt ggcctgttaa
     1021 catgagtgtt gcagaagtgc ctgattcgga ggaaaaccgc aaggcagaca gctcagggcg
     1081 ctggaagttt ga
//
LOCUS       DAZGVQ010000385         1091 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000385 DAZGVQ010000000
VERSION     DAZGVQ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.389"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..262
                     /locus_tag="ACHLAG_005408"
                     /pseudo
     CDS             1..262
                     /locus_tag="ACHLAG_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1327 domain-containing protein"
     gene            685..>1091
                     /locus_tag="ACHLAG_005409"
     CDS             685..>1091
                     /locus_tag="ACHLAG_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP0658444.1"
                     /translation="MNAKIRYGLSAAILALITAGASAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATVVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGA"
BASE COUNT          292 a          235 c          292 g          272 t
ORIGIN      
        1 gggtgggtat acgtcactgt tgttgccaaa tgtggtgatt gatcttgatc gtgttgaagg
       61 tgccccgctg gagttttacg aagctgaggc gaaaaagaag gcgaagcagt ttttcatgga
      121 tattgctgcc gggttatgtg aaggggatga gcagtcgccg gaaaagcgcc ccataatttt
      181 agaggcgcag gatgtgttga taacctacag aggaaaacta ccgggaataa ttactggttc
      241 tctgaagacg ccaccgacgg cattgcggtc agaaaacgat gatattgaat cacgcattga
      301 aaaactggag tgctatatcg ctgaattgaa aaaaagcacc ccaacaaaaa atgaggtgct
      361 tgcagcagat gaaatgaaag aaactattct tgatcgcgcg gcgcatctaa gctgcgcttc
      421 actgttgaaa gagcatcttc agcagccttg aggaatcttt tatcatcacg agcaactgtc
      481 tttggtattt ctttaattag atgttccatt tcggcgatca ggtaatctga cacagcgtta
      541 tttttactta gtgcatccat tgctttgatt atctttgcga ggcagccatc tcgcattgtt
      601 gagtttacgc cagtgcccac cactggcggg ctgaagactt aacatatcca gggattcgga
      661 accgataaat cctgataaat atccatgaac gcaaaaatca gatacggcct gtcggctgcc
      721 attctggcgc tgattactgc aggcgcatct gcgcctgaca ttctcgacca gtttctggat
      781 gaaaaagaag gtaaccacac cacagcatac cgtgatggtt ctggtatatg gaccatctgc
      841 cggggtgcca cggtggtgga tggcaaacct gtcgttccgg gcatgaagtt gtcgaaggaa
      901 aaatgcgacc aggttaacgc cattgagcgt gataaagcgc tggcgtgggt ggagaaaaac
      961 atcagagtgc cgctgaccga accccagaaa gcggggatcg cgtcattctg tccgtacaac
     1021 attggtcccg gtaagtgttt cccgtcgacg ttttacagac gaattaatgc tggtgatcgc
     1081 aggggagcat g
//
LOCUS       DAZGVQ010000386         1089 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000386 DAZGVQ010000000
VERSION     DAZGVQ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.390"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            59..469
                     /locus_tag="ACHLAG_005410"
     CDS             59..469
                     /locus_tag="ACHLAG_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658445.1"
                     /translation="MAKRKNNVVKKIGDSASLLSKPKSILRRKDFKELVTLTKQNSAP
                     GEWKTEIIEHSPSVPCGDEYIALQEILSSTPGVFWKPRKRKEYIVDSSDLRKYQILGF
                     EDYNHYVGYLATNGLNNLVPEFQILDNADHYGDF"
     gene            471..809
                     /locus_tag="ACHLAG_005411"
     CDS             471..809
                     /locus_tag="ACHLAG_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658446.1"
                     /translation="MNKNTYDTIYSLINYYEDDYLLPLNRAELEAYKENTPSALNEAF
                     KHWDLAVNAFEHLSKRVEMLCKRENAYLTADQIWELSNWIEDIESDVRYVGDGLVELA
                     QRLGATITEE"
BASE COUNT          363 a          200 c          197 g          329 t
ORIGIN      
        1 ctcacgttag aagcatattt cggggtgttt tcctttaaaa gaaaaggaaa aataaattat
       61 ggcaaaaaga aaaaacaacg ttgttaagaa gatcggtgat tcagcttctc ttctaagtaa
      121 acctaaatct attctaagac gtaaagactt taaagagcta gttacgctca ccaagcaaaa
      181 tagcgcccct ggcgaatgga aaacagagat catagaacat tctccctctg tgccttgtgg
      241 agatgaatat atcgcattac aagaaatctt atcttcaaca cctggcgtat tctggaaacc
      301 tagaaaaaga aaagagtata tcgttgatag ctctgattta cgcaaatacc agattttagg
      361 atttgaagat tataatcact acgttggtta tctcgccacc aatggcctaa ataatttagt
      421 tcctgaattc caaattttag ataatgctga tcactatgga gacttttaat atgaacaaaa
      481 acacgtatga cacaatttac tcactaatta attattatga ggatgattat ctcctgcctt
      541 taaaccgcgc tgaactcgaa gcatacaaag aaaacacccc atcggcacta aatgaggcgt
      601 ttaaacactg ggatttagct gtgaatgcct ttgagcacct ctctaagcgt gtagagatgc
      661 tctgtaagcg cgaaaacgca tatctaacag cggatcagat atgggagtta tcaaactgga
      721 tagaagatat tgaatctgat gttcgctatg taggagacgg ccttgttgaa ctagctcaac
      781 gcttaggcgc tactatcaca gaagaataaa aaaagaaaca aaaaagttgt aaaatatctt
      841 gacattccat ctattgtatg ttaatataat tatatggggt gattattttt cctttattgg
      901 ttcgatatta aaaattcttc ttgtcgttaa aggggagtaa aatctccgct ccccgcttct
      961 cctcctcctt aagtgtcatt attacctctt ttttcctttg ttatgcatac atcttggagt
     1021 atgcataaca tagggtaatt acataggtac aagttaataa aaggcggctc taaatgctgc
     1081 cttttattt
//
LOCUS       DAZGVQ010000387         1079 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000387 DAZGVQ010000000
VERSION     DAZGVQ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.391"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..573
                     /locus_tag="ACHLAG_005412"
     CDS             <1..573
                     /locus_tag="ACHLAG_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP0658447.1"
                     /translation="YLAEMLYLPIDLIESFQKLYTVTDQIRNKTSFFLPQNPELIYCW
                     EQLKTSVSRGFSTKIQEHLAMGVLLSLGVNHVNHLLLSYSKQSLISRCYNLLLSEPGT
                     KWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQTTVKPISEIARE
                     NGYKCPSRFTERFHNRFNITPREIRKASRE"
     gene            690..791
                     /locus_tag="ACHLAG_005413"
     CDS             690..791
                     /locus_tag="ACHLAG_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658448.1"
                     /translation="MRTYNPNSLLPSQMQKCTCNSLHLAFDLCGGEA"
     gene            788..>1079
                     /gene="ybcN"
                     /locus_tag="ACHLAG_005414"
     CDS             788..>1079
                     /gene="ybcN"
                     /locus_tag="ACHLAG_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFP0658449.1"
                     /translation="MNLSQDGIKLHRGNFTAIGRQIQPYLEEGKCFRMVLKPWREKRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKDLVDVVTG"
BASE COUNT          328 a          232 c          220 g          299 t
ORIGIN      
        1 tatcttgcag aaatgcttta cttacccatt gatttaattg agagtttcca aaaactttat
       61 acggtaactg atcaaatacg taacaaaaca agtttctttt tacctcagaa tcctgagtta
      121 atatattgtt gggagcaact aaaaacatct gtttcccgag gcttctcaac taaaattcag
      181 gagcacttag caatgggcgt tctactttcg ttaggagtga atcatgttaa tcatttactt
      241 ttatcatata gtaaacaatc attgataagt cgttgttata acctgctgct atccgaaccc
      301 ggcacaaaat ggacagcaaa caaggttgct cgatatctct acatttctgt ttctacatta
      361 catcgccgtc tagcaagcga gggggtaagt ttccaaagta tactggacga tgtgaggtta
      421 aataatgcgt tgtctgctat acaaacgacg gtaaaaccta taagcgagat tgccagagaa
      481 aatggttata agtgtccttc tcgttttact gaaagatttc ataatcgttt taatataaca
      541 ccaagagaga taagaaaagc ttccagagag taaaagtgtt ttaagaagga gcaattctat
      601 cgattttgat tttgggaaat caacacggca taattatgtc accggagcct gaacaactcc
      661 ggtgacttct gcgctaaacg gggacgttta tgcgcacata caatccaaac tctcttctcc
      721 cttcacagat gcagaaatgc acctgcaatt ctttgcatct agcgtttgac ctctgcggag
      781 gtgaagcgtg aacctctcac aagacggcat caaattacat cgcggcaact tcaccgctat
      841 cggtcggcag atccagcctt atctggagga gggcaaatgc tttcgcatgg tgcttaaacc
      901 gtggcgtgag aaacgcagtc tttcccagaa tgcactcagc cacatgtggt acagcgaaat
      961 cagtgaatac ctcatcagca ggggtaaaac gttcgccact ccagcttggg taaaagatgc
     1021 tctcaaacac acatatctcg gttatgaaac caaagacctg gttgatgtcg taaccggtg
//
LOCUS       DAZGVQ010000388         1078 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000388 DAZGVQ010000000
VERSION     DAZGVQ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.392"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..381)
                     /locus_tag="ACHLAG_005415"
     CDS             complement(<1..381)
                     /locus_tag="ACHLAG_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP0658450.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     VGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRI"
     gene            389..814
                     /gene="tnpA"
                     /locus_tag="ACHLAG_005416"
                     /pseudo
     CDS             389..814
                     /gene="tnpA"
                     /locus_tag="ACHLAG_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          287 a          264 c          238 g          289 t
ORIGIN      
        1 aatacggctg ttttcctgat cgagcttaac gccctgcggg tagcggaata catcattctg
       61 tccccgcttt ttgaatcggg gaaaagcagc ccgcttctgg aagaagtttt tgtaagcccg
      121 ctcaaggtct ttcagtgact gttgcaatgg ctgtgacggg gcatctttaa gccattgcgt
      181 ttcagtggca tttttccact caaccagcca ggaagccatt ttcccgtaag ggatgtattt
      241 attaccgacc tcatgattct cattctgacg tgccagagca cgattgaaaa cgaaacgaca
      301 tgcgcctgcg aagcgcctca tttcacgctc ctgttgacca ccgggtctta actggaattt
      361 aaatgcctgt aatcgtttca caggccaaat gatactttgg tctgtgaaaa aagaaaccga
      421 tatccggcgt ggaaggcatt gtgttttcct gatgcatgtt cacctggtct ttgtcaccag
      481 ataccgacgt cagatttttg accatgacgc gacagaaaaa ctacgcactt acttttcaaa
      541 tgtatgtgct gattttgaag ctgaactggt tgaaatggat ggcgaaccag atcacgtcca
      601 tttgttaatt aactatcctc ccaaactggc gatatccagt ctggtaaaca gcctcaaagg
      661 cgtatcgggt aggtgactgc gacgagatcg accagatatt gcagtcaggt attactacaa
      721 aggcgttttg tggagtgctg gctattttgc cagtagctgc ggaggtgcgc caatatccgt
      781 catccgccaa tacattgaac aacagcaaac acctggtcag gtggaaaacc gcgccttata
      841 tccctgccct gaaggacggg gttttacggc gcaccggata agcctggaga ttatctacac
      901 cagcatggac atcattgtac tatgccccgt acatgcccca tcacatcctc aacatcaaag
      961 tattagagcc ttgcaaacca ttatgcgtct cagtttgtct catctatctt acgcaaaaaa
     1021 aatagaacta atatcaagcg gcgctatatt gcgtattaaa taaaaccacc tcaattac
//
LOCUS       DAZGVQ010000389         1059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000389 DAZGVQ010000000
VERSION     DAZGVQ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.393"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1059
                     /locus_tag="ACHLAG_005417"
     CDS             <1..>1059
                     /locus_tag="ACHLAG_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658451.1"
                     /translation="LSSSLAGLLVMSGGVSAQEEKYTVPYATGNNKWGNTYEVVKTGD
                     NGNFKYEVKEKKNGRKTLFTFDSNGDVIINGSGITYTIHDGALNDFARAAEKKKNGQS
                     QSHRMTDSVVRDVYNKVYSLQRTEITGFSVEDGENGKVSLGSDAKASREFSVAVGNGA
                     KATGKASTSVGSWSSAFGTASVAVGGPATQAYGDRSTSVGVGASVRPNATEGTAVGFK
                     ASVDENAADGVAIGASSTVAHRNSVALGSNSRTTREDEVYIGYEAEPGKAYKTRVLGG
                     LSDGTRPSDAATVRQVDRVKDSVEQLAQDTNTRLVVEAKKSREYTDSRTTVGVNSDGT
                     LTSADGATKTIAVNDGLVA"
BASE COUNT          299 a          205 c          299 g          256 t
ORIGIN      
        1 ctgtcatctt ctctggccgg gcttctggtg atgagtggtg gggtgagtgc gcaggaagaa
       61 aaatacacag tgccgtatgc cacaggtaac aataaatggg ggaataccta tgaagttgta
      121 aagacaggcg ataatggtaa cttcaaatac gaagtaaaag agaaaaaaaa cggccggaag
      181 acacttttca catttgattc aaatggggat gtaataatca atggcagtgg cattacgtac
      241 acaattcatg acggtgcgct gaacgatttt gctcgagcag ctgagaaaaa aaagaatgga
      301 cagtctcagt cgcacagaat gactgatagc gttgttcgtg atgtatataa caaagtatac
      361 agtctgcaga gaacagaaat tacaggtttc agcgtcgaag atggggagaa tgggaaggtt
      421 tctctgggta gtgatgcgaa agcctctcgt gagttctcag tagctgttgg taacggtgct
      481 aaggctacag gaaaagcgtc aacatctgtt ggctcatggt catcggcatt tggaactgca
      541 agcgtggcgg ttggtggccc tgcaacccag gcatatggtg atcgttcaac atctgttggt
      601 gttggggcat ctgttcgtcc aaatgccaca gaaggaacag ctgttggttt taaggcaagt
      661 gttgatgaga atgcagctga tggagttgca ataggtgcca gctcgacagt tgcccacaga
      721 aacagtgtgg ctcttggctc aaattcacgt acaacccgtg aagatgaagt ttatataggt
      781 tacgaagctg agccgggtaa ggcttataaa acccgggtgc ttggtggtct gagtgacggt
      841 actcgtccct cagatgctgc aaccgttcgt caggttgacc gtgtaaaaga cagtgttgag
      901 caactggctc aggatacgaa tacccgcctt gtggttgaag cgaaaaagtc tcgtgaatat
      961 acagactcca gaacgacagt tggtgttaac tccgacggaa ccctgaccag cgctgatggc
     1021 gctacaaaaa cgattgctgt taatgacggt ctcgttgct
//
LOCUS       DAZGVQ010000390         1048 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000390 DAZGVQ010000000
VERSION     DAZGVQ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.394"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            22..>1048
                     /locus_tag="ACHLAG_005418"
     CDS             22..>1048
                     /locus_tag="ACHLAG_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0658452.1"
                     /translation="MKKAIAYMRFSSPGQMSGDSLNRQRRLIAEWLKVNSDYYLDTIT
                     YEDLGLSAFKGKHAQSGAFSEFLDAIEHGYILPGTTLLVESLDRLSREKVGEAIERLK
                     LILNHGIDVITLCDNTVYNIDSLNEPYSLIKAILIAQRANEESEIKSSRVKLSWKKKR
                     QDALESGTIMTASCPRWLSLDDKRTAFVPDPDRVKTIELIFKLRMERRSLNAIAKYLN
                     DHAVKNFSGKESAWGPSVIEKLLANKALIGICVPSYRARGKGISEIAGYYPRVISDDL
                     FYAVQEIRLAPFGISNSSKNPMLINLLRTVMKCEACGNTMIVHAVSGSLHGYYVCPMR
                     RLHRCDRP"
BASE COUNT          346 a          163 c          237 g          302 t
ORIGIN      
        1 catacgttag caggtgtcac catgaaaaaa gccatagcat atatgcgatt ttcatcacca
       61 ggtcagatgt ctggtgactc attaaaccga cagagaagac ttattgctga atggttaaag
      121 gtaaatagtg attattatct tgataccata acatatgaag atttaggatt aagtgcattc
      181 aaaggaaagc atgcacaatc aggagctttt tcggaatttt tagatgctat agagcatggt
      241 tatatattgc caggaactac attgttagtt gaaagtctgg acagactttc aagagaaaaa
      301 gtcggtgaag cgattgaacg tctgaaattg attttgaatc acggtattga tgttataact
      361 ctttgcgaca atacagtcta taatattgac tctttgaatg agccatattc attaataaaa
      421 gccatactta tagcacaaag ggcaaatgaa gaaagcgaga taaagtcaag tcgggttaaa
      481 ttatcatgga agaaaaaacg gcaggatgca ctggaatcag gtacgattat gacggcgtct
      541 tgtccgagat ggctctcctt agatgacaaa agaacggctt ttgttccaga ccccgacagg
      601 gtgaaaacta ttgagctaat ttttaaactc aggatggaaa ggcgctcatt gaatgcaata
      661 gccaagtatt taaatgatca tgctgtaaag aatttctcag gaaaagaaag tgcatgggga
      721 ccttctgtaa ttgaaaaatt attagcgaat aaagctctga taggtatatg cgtaccttca
      781 tatcgtgcaa gagggaaagg gataagtgaa atcgctggct attatcccag agtcatatca
      841 gatgatttgt tttacgctgt acaggaaatt cggttggcac cttttggtat tagcaatagt
      901 agcaagaatc ctatgctaat aaatctactt cgaacagtta tgaagtgtga ggcttgtggt
      961 aataccatga ttgttcatgc ggtatctgga agtttgcatg gctattatgt ttgtccgatg
     1021 agaagattac atcgatgtga caggccat
//
LOCUS       DAZGVQ010000391         1045 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000391 DAZGVQ010000000
VERSION     DAZGVQ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.395"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..1042
                     /locus_tag="ACHLAG_005419"
     CDS             2..1042
                     /locus_tag="ACHLAG_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFP0658453.1"
                     /translation="MKRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALRE
                     YNEMVDDYNSLR"
BASE COUNT          332 a          220 c          256 g          237 t
ORIGIN      
        1 aatgaaaagg aatttattat cttccgctat tatagtcgcc atcatggccc tcggtctgac
       61 gggttgtgat gataaaaaag ccgaaacaga aacgcccccg cctgccaata gtcaacctgc
      121 cgcaccagct cctgaagctc ccgttgcaaa agcagaagct aaacctgaaa cacctgcgca
      181 accggtggtc gatgaacaag cggttttcga cgaaaaaatg gatgtctata tcaagtgcta
      241 taacaagtta cagatccctg tacagagcag tctggcgcgt tatgctgact ggctgaaaga
      301 ttttaaacag gggcctaccg gtgaagagcg tactgtttat ggcatctacg gcattagtga
      361 atccaacctc gctgagtgtg aaaaaggcgt aaaaagtgct gtggcgttaa cgcctgcgct
      421 gcaaccaatt gatggcgtgg cggtgagtta tatcgatgct gccgtggcgt tgggtaatac
      481 cattaacgaa atggataaat attacaccca ggaaaattat aaagacgatg cgtttgcgaa
      541 agggaaaacg ctgcaccaga cattcttaaa aaatctggaa gcctttgaac ctgtagcaga
      601 atcttatcat gcggcgattc aggaaattaa cgataagcgc cagcttgcgg aactgaaaaa
      661 tattgaagaa agagaaggga aaacattcca ctactactct ctggcagtca tgatttcagc
      721 gaaacaaatt aataatctga tatcgcaaga gaagtttgat gttgatgcgg caatgaagaa
      781 agtgtctgaa ctggaaacgc tagtggcgca ggccaaagaa gcggataaag gcggcatgaa
      841 tttctcgttt attaattcgg caggccagta tcaactcgag gctaaaaaat acgttcgccg
      901 catcagagat aaagtcccgt actctgactg ggataaagag caacttcagg acgcaaactc
      961 aagctggatg gtcgaagact ctttcccgag agcattacgc gagtacaacg aaatggttga
     1021 tgactataat agcctgcgtt aaacg
//
LOCUS       DAZGVQ010000392         1031 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000392 DAZGVQ010000000
VERSION     DAZGVQ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.396"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..492)
                     /gene="sctT"
                     /locus_tag="ACHLAG_005420"
     CDS             complement(<1..492)
                     /gene="sctT"
                     /locus_tag="ACHLAG_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFP0658454.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWMFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNF"
     gene            complement(494..754)
                     /locus_tag="ACHLAG_005421"
     CDS             complement(494..754)
                     /locus_tag="ACHLAG_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP0658455.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(764..>1031)
                     /locus_tag="ACHLAG_005422"
     CDS             complement(764..>1031)
                     /locus_tag="ACHLAG_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP0658456.1"
                     /translation="DNISIFSLLPAYALSEIKSAFIIGFYIYLPFVVVDLVISSVLLT
                     LGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLSINP"
BASE COUNT          368 a          225 c          171 g          267 t
ORIGIN      
        1 aaaattaaaa ttgcctgaag gccataaatt ataactttgc caaagagttt ccagaataaa
       61 gttcattcca cctttagtaa gataaaccgc agcagagaaa aggttaaata atcttgctaa
      121 ttccgaggta tcgacacctg tagccggatc caaagtacta cttaatgttg caccgcgttg
      181 gttatcgata aagctaccta tagcaaggaa catccaaaac ggggatgcta ataagcatgc
      241 aataaacagc cctatgataa tttctctggc agcgaacaaa gcaatatcga tttcatttaa
      301 gttcaataat tcatagtggt aatgctgaac taatgcacat gataccacta ttattattgg
      361 gtgacgaact actgttggta tattcccact ggcgaaaaat ggtaacaaat agaaagttgg
      421 tgccaatcgg caaaaaccga gcgctgtagc agccagcaga ctatgcaact gatagagtat
      481 cgcctccccc atgctatcct ctcgcgaatg ctaaattcag catttcgata ccaaatgaat
      541 ataatttttc accataccac ccgctcatta aaaaaaagca aatacttacg cacaataatt
      601 tcacgccaaa tggcaacgtc tgttcttgca actgcgttac cgtttgaaaa agccctacca
      661 ataaaccaac aaatgtcgca actgcaatag gtcctgcaga cataactaaa atcagataca
      721 gcgccctatt ccccgcaaaa acaatatcgt ccatattttt tcctcaggga ttaatggata
      781 ggtcaaaata ttgtaaaatg agcccttttg ataacattgt ccaaccgtcc atcgcgacaa
      841 acaaaattaa ttttatcggt gtagatatgg ttaccggact catcatcatc atacctaacg
      901 ttaacagaac gctggatata accagatcca ctacgacaaa gggtaaatag atataaaaac
      961 ctataataaa tgcacttttt atctcactca atgcatatgc gggcagtaac gagaaaattg
     1021 aaatattatc a
//
LOCUS       DAZGVQ010000393         1029 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000393 DAZGVQ010000000
VERSION     DAZGVQ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.397"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(29..901)
                     /gene="ygcG"
                     /locus_tag="ACHLAG_005423"
     CDS             complement(29..901)
                     /gene="ygcG"
                     /locus_tag="ACHLAG_005423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP0658457.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLDSIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVYISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(915..>1029)
                     /gene="yqcG"
                     /locus_tag="ACHLAG_005424"
     CDS             complement(915..>1029)
                     /gene="yqcG"
                     /locus_tag="ACHLAG_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HFP0658458.1"
                     /translation="FNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFIQI"
BASE COUNT          331 a          194 c          207 g          297 t
ORIGIN      
        1 ctccgggctg gcatttttta tggttgagtt accagcggcc ggatgcgccg ccacctccag
       61 aagaaccgcc accgccgcta aatccgccgc cacttgagcc tccaccggaa ccgctgttgt
      121 ctgaatgata acttttacct gatgctcttt tctgaagtac acataaacag gtaaagacca
      181 taaatatggt gaaagtaaaa aagaaggaaa atacagcaat ggaaaaaatt ttatcggaga
      241 acaggaataa atctaaaaga tagattgctg aaatatagac tccactttta catgcgcgac
      301 aaaaattact gccttgatga aaaaaccaga aagggaagaa catcactgca aatacccaaa
      361 aaatagcaaa gtaataatga tcactggaag acgctgattc actttctgaa ggattagtcg
      421 gatattggtg ttgtgaagtg agttggttat tcaaagcgtt tatagctagc tctaatccct
      481 gagcaaattt ttgttgttta aatgcgggta tcatgttgct acggatgata tctccagcca
      541 gagcatcggt taccttttct tccagcccat aacctacctg gatgcggaca gtacgatctg
      601 accaggcaac aatgatcagt atcccatcat tacgtttggc atctcctaaa cgccaattgt
      661 caaaaactct tgtagcatat tgttcaattg tctcgtcttt ggttgtggga acaattaata
      721 cagcaatttg cacttgggta ttattgaaaa tagagtctaa cttgtgcgtc agttctttct
      781 tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg ttgctgtaat tgtgggacaa
      841 tagttggttg ggctgcaaca aaggaacaca cgaaagtgaa cattagtatg aaatagcgca
      901 ttataaattt ctcattaaat ctggataaat aaattgacgg cggcatcggt aattttagat
      961 tcgttatcat taaaaactaa cacagaaaaa ataaatataa ttttggtgaa tgttttgaca
     1021 tgattaaat
//
LOCUS       DAZGVQ010000394         1024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000394 DAZGVQ010000000
VERSION     DAZGVQ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.398"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            173..>1024
                     /locus_tag="ACHLAG_005425"
     CDS             173..>1024
                     /locus_tag="ACHLAG_005425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HFP0658459.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVINPALGKAFAQSEGLRNKTD
                     TVDARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQELNRTETAREVQR
                     PSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGL
                     KDRFAHARQFAAFAGLTPRRYESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEW
                     GRAFRDRL"
BASE COUNT          246 a          256 c          295 g          227 t
ORIGIN      
        1 agagaaaatc ggccagtttt ctctgcctgc agtccgcatg ccgtatcggg ccttgggttc
       61 taacctgttg cgtagattta tgcagcggac tgcctttctc ccaaagtgat aaaccggaca
      121 gtatcatgga ccggttttcc cggtaatccg tatttgcaag gttggtttca ctatggaaca
      181 tgaacttcat tatatcggta tcgacaccgc taaagagaaa ctggatgtcg atgtgttgcg
      241 tcctgatggt cgtcatcgca ccaaaaaatt cgctaacacc actaaagggc acgatgagct
      301 ggtgagctgg ctgaaaggtc acaagattga ccatgcgcat atctgcatcg aagcgaccgg
      361 cacctatatg gaacctgtcg ctgagtgcct ttacgatgct ggctacatag tgtcagtcat
      421 taatcctgcg ctgggtaaag ctttcgctca gagtgaagga ctgcgtaaca agactgatac
      481 cgtggatgcg cgcatgctgg cagagttctg tcgtcagaag cgccctgcag cctgggaagc
      541 gcctcacccg cttgaacgcg cgttgcgtgc cctggtagtc cgccaccagg cgctgacaga
      601 tatgcacacg caggaactga atcgcactga aacggcgcgg gaagtccaga gaccgagcat
      661 tgatgctcac cttctgtggc ttgaagcaga gctgaagcgt cttgagaagc agataaaaga
      721 cctgacagac gatgatccgg atatgaaaca ccgcaggaaa ctgctggaaa gcatcccggg
      781 tatcggagag aaaacatctg cggtattgct ggcttatatc ggtctgaagg accgcttcgc
      841 ccatgccaga cagttcgccg cttttgcggg tctgacacca cggcgttatg aatcaggtag
      901 cagtgtgaga ggggcgagcc ggatgagtaa ggccggacat gtgtcgcttc gcagggcgtt
      961 gtatatgccc gcaatggtag ccaccagtaa gactgagtgg ggacgggcgt tccgcgaccg
     1021 tctg
//
LOCUS       DAZGVQ010000395         1016 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000395 DAZGVQ010000000
VERSION     DAZGVQ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.399"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..828)
                     /locus_tag="ACHLAG_005426"
     CDS             complement(<1..828)
                     /locus_tag="ACHLAG_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFP0658460.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKEPWPFNYVTETCRESDGITTTGRMLYRNLKI
                     TTVLDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVSGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
BASE COUNT          300 a          191 c          192 g          333 t
ORIGIN      
        1 tttaccaata taattccatt caccattttt taattcaaac agttctggaa gagatgacgg
       61 ttgcagagtc atacgtttga gtggtgcatt ttcatcaccc gggataactt tatatcccat
      121 ttttgcatgg atctccgcgg taccgggata ggcttctata ccaattattg ggtcattacc
      181 caactcaata ttagcattaa gtaatgcggc aagggcgaat ccggaagaat ttgccacccc
      241 tgagacagtc attatctgat caatatagat atcaatcctt ggctgctcat tggtccatct
      301 ttttgcctga gaagctggaa gggttgaaat atatcctatc gtggttccgg cataaccagg
      361 aacattgcaa ccagggccta atgttatttc atgaactgta tagtcctcaa caggatcgaa
      421 agggctcttc ataaataaat ttagccgtaa tccttctgct aactctgtgg cagcatgcgc
      481 ctttttacat ataccaccat atatttcgtc taaaactgtg gtgattttca agtttcgata
      541 gagcatacgt cctgttgttg taataccatc ggattctctg catgtttctg tcacataatt
      601 aaatggccag ggctctttac taatatcaag ttttctaatt tcgacaacgc tttttccatc
      661 gtaattatgg ttattgccac gagcaagagg cagaagatag atattttccg tatccaggcg
      721 tgcatattca tatcgacgtg ttaactcact gtcgactttt ggatgatgaa ggaagtgatt
      781 atctgtaata atttgactct gaagggcatt gcttaatggc atagacataa tagttacctt
      841 ttatgagtat ttcactgatg tttagaaaaa tagataaatt tttctgtagt aaaaaagaag
      901 taaacaaatg acatgcatgt ttctgttgta gtgatatcaa ctctacatgg tgatattaaa
      961 gggtaggaaa cactctaaag tatcaaaaaa cgctcattta aaattatttt catgca
//
LOCUS       DAZGVQ010000396          997 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000396 DAZGVQ010000000
VERSION     DAZGVQ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.400"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            86..>997
                     /locus_tag="ACHLAG_005427"
     CDS             86..>997
                     /locus_tag="ACHLAG_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFP0658461.1"
                     /translation="MKKMTVALSAVAVAVMFAAGAQAAEVYNKDGNKLDLYGRATALH
                     YFSDDKGNDGDQTYARLGFKGETQINDQLTGFGQWEYQFSGNKTESEGSAGNKTRLAF
                     AGLRFADVGSIDYGRNYGIAYDVGSYTDVLPEFGGDGWTQTDNFMTARTSGVLTYRNT
                     DFFGLVDGLNFAAQYQGKNERDDLQKANGDGYGFSASYEFDGFGLVAAYTKSDRTDKQ
                     VNGGSSVASGKYAEMWGAGLKYDANNLYIATIYSETQNMTAFGDKVNGIADKAENVEA
                     VAQYQFDFGLRPSLAFLQSRGQEVIVGG"
BASE COUNT          270 a          174 c          268 g          285 t
ORIGIN      
        1 aactaagaat ttgtattaaa atattctaat ttttgttcat gacatctgaa tgcatgaata
       61 gttcagttta aataaggatt aaatcatgaa aaaaatgaca gtggcacttt ctgctgtagc
      121 agttgcagtg atgtttgctg cgggggcgca ggcagcagaa gtttataata aagatggtaa
      181 taaactggat ctttacgggc gtgcaaccgc tctgcattac ttctcggatg ataaaggtaa
      241 tgacggagat cagacttacg ctcgtctcgg ctttaaaggc gaaacgcaga ttaatgatca
      301 actgaccgga tttggtcagt gggaatacca gttctctggt aacaaaacgg aatctgaagg
      361 ttccgcggga aataaaaccc gtctggcatt tgcaggtctg agatttgctg atgttggtag
      421 cattgattac ggtcgtaact acggtattgc ttacgatgtc ggatcatata ctgacgtact
      481 gccagagttt ggtggtgatg gctggactca gaccgataac tttatgactg cccgaacttc
      541 cggagttctg acctaccgta atacagattt ctttgggctg gttgatggtc tgaattttgc
      601 ggcgcagtat cagggcaaaa atgagcgcga tgacctccag aaggccaatg gtgatgggta
      661 tggtttctca gccagctatg agtttgatgg ttttggtctc gtagcggcgt acacgaaatc
      721 tgaccgtact gataagcagg ttaatggagg aagctctgtt gccagtggta aatatgctga
      781 aatgtggggg gctggtctga aatatgatgc caataatctg tatattgcga caatatattc
      841 agagacccaa aatatgacgg cttttggcga taaggttaac ggcattgcgg ataaggcaga
      901 aaacgttgaa gctgtagcgc aatatcagtt tgattttggt ttacgtccat cactggcatt
      961 cctgcagtct cgtgggcagg aggttatagt tggtggg
//
LOCUS       DAZGVQ010000397          995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000397 DAZGVQ010000000
VERSION     DAZGVQ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.401"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(124..279)
                     /gene="hokD"
                     /locus_tag="ACHLAG_005428"
     CDS             complement(124..279)
                     /gene="hokD"
                     /locus_tag="ACHLAG_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP0658462.1"
                     /translation="MKQQKAMLIALIVICITVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TVYEPEE"
BASE COUNT          264 a          264 c          208 g          259 t
ORIGIN      
        1 cgtgcgaatt gtaaaccaga atgccaggaa aaaacaaaac ccgacgaagc gggttaagcg
       61 cgggtgcgtt gaggatgcct gacacatcag aggtggcggg agatttctcc cccgcctggt
      121 ctcttactcc tcaggttcgt agactgtgaa gacagcgacc tccgtctggc cggttcggat
      181 tcgtacctcg cagaggtctt tcctcgttac cagtgccgtc acaatgacgg taatacagat
      241 gacgatcagg gcgattaaca tcgccttttg ctgcttcata gcctgcttct ccttgacctt
      301 tcggtccgta agaggctaac ctacgtgtgt agagcataga tatggcctca gattaatgtt
      361 aagcgtcttg ccggacgcgt aatgttaact ggggcttttc tctatctgcc ttttggtgtt
      421 catgcctgag acagatagcc tcaagcaccc gcagcgattt tacagaaata agttattatt
      481 ttctatctca cccttcactt atctcattcc tagctatact catcgttcac acaacattaa
      541 agggcataaa aatgaccgtt catggtatac aggaaggcag tattgttaac ttacgtgaaa
      601 aagatgggga atggcttgta gtacaaacta ttaaaaacga cgaccttaca tatcaactcc
      661 gaaatacaga taccaacgaa gaagtaaacg tcgcacttca ggacattatc tccccaaata
      721 aataatcaaa tcagcggggc gcacgatacc ccgcactatt ccacttatca ccccagcggc
      781 aaatcgaata caccaccagc gccaccgcca ttgcaattcc taccgttgtt aatgcttcag
      841 gccaggtcat cgtaaaatat cctccacgct tatcagtccg ttccgctcca gataactcat
      901 cgccttatcc ggtaatttgc agtctggctt cgctttcctc agttgccagg ttaactgctt
      961 taccagcatg gttaactcat cgaccagacg ctgat
//
LOCUS       DAZGVQ010000398          995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000398 DAZGVQ010000000
VERSION     DAZGVQ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.402"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(806..>995)
                     /locus_tag="ACHLAG_005429"
     CDS             complement(806..>995)
                     /locus_tag="ACHLAG_005429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658463.1"
                     /translation="CSVTSKINIKNSAKFNIRSTSRLTYDIISQLHLPQNGYFMERSN
                     FLVAIIMLILRSLAIIPG"
BASE COUNT          309 a          173 c          198 g          315 t
ORIGIN      
        1 tatttaaata aacagagagc cagattaaat acttgagtat aaaatctctg ctgatgttta
       61 attgatttga atgttcgtaa gctatatcac ttactcaatt cattttaccc agagtcataa
      121 aatagagtgt ctgttgatct cattttcata ttcctttcag actgaagtgc attttcatca
      181 cccgatgaga aagaactgaa gtgatgtttg ccatagatta cgagatgccc agtctcattg
      241 cctatcgtgg tgtaccttta tacatgttat accagatcaa aaatcacgca atccatacaa
      301 cgaaaccaga tttgcaattc gtgtcacaaa atatgtcgat ctttttctaa gaagaagatg
      361 ccatgtgaag ccagacgaac acttgcggag gccttcacaa accaaagatt gttgtttaat
      421 tatctgtttt ataagtaatt tatttaaagt ataaacaagg aaagatttgc aagctaactc
      481 agtgagcgtg gtgaaaatca gtgtttaccc gccatcaggc tgagcataat tctcatcatg
      541 aaatatgttt cctggtttgt ggcttgtaac tggtcacttc tgaagtcgat ctggagaggc
      601 ttgttgatgt tggtgttttc aggatgatgt ttcacttagt ttgtttgccg tatcgcccgg
      661 cgaatggctg tgattgagga aggtttaagt cgtagtgacc aaagctatat ttaccaacga
      721 atgtagatga aaaaatcatc tcctgcgttc cccatatctc caggataaaa aggaatgtaa
      781 caatctcatg gcgtaagctg acgaatcagc caggaataat cgctagggac ctaagaatta
      841 gcatgataat agccactaag aaattactgc gctccatgaa atagccattt tgtggcaaat
      901 ggagttgact aataatgtca tatgtgagac ggctagttga acgaatatta aattttgctg
      961 aattttttat gttgatttta cttgttacag aacat
//
LOCUS       DAZGVQ010000399          982 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000399 DAZGVQ010000000
VERSION     DAZGVQ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.403"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..236)
                     /locus_tag="ACHLAG_005430"
     CDS             complement(<1..236)
                     /locus_tag="ACHLAG_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFP0658464.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASNKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLS"
     gene            complement(871..>982)
                     /locus_tag="ACHLAG_005431"
     CDS             complement(871..>982)
                     /locus_tag="ACHLAG_005431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFP0658465.1"
                     /translation="YWMEFAVNNGNIDTKSKLQELKKLKRMDRRKNKENP"
BASE COUNT          286 a          213 c          167 g          316 t
ORIGIN      
        1 ttagataagg atgtgagaaa ttccttgtta tcattcgcag ccatatatgc cagtccgagt
       61 tcagcctctg cggccttaca ggcaaaatag cttagtgaac acgcttttcg tccctcttta
      121 ttactggctg atattagtgc ccataatatt gctttgtctg ccaaatctaa ctctctctga
      181 aattgtcttg cagtaactgc actcgcagaa atagagaaaa aaatacaatt gttaataacc
      241 ttgccatatt ttttagtccg taactgttaa tgtgctatct ggctctgaaa atattaactc
      301 atttaatttt tgcatgtcat tagataaacc agttatacaa ccctcggaac agaatcgagg
      361 tcctaaagac ggaccaaaag cgtttaaaca actatgtatt gaaaatccat tgcgattata
      421 agttcctgta ttaactgacg gtactaatcg tatgccgtca ggttttctat tgcttgctcc
      481 cgggtcatta acattccaac tccataggcc ttgaggtatt ggccatctat ttataaatgt
      541 gtacaacggg gattattttt acattgcata ttattaccag aagcgactgg aatactaagc
      601 acatcataaa aattatcatc acatggtata tctgaaacac acgaaagcat acctaaagtt
      661 atactgtata aacaggtggc taatcccaag gggatcgatt ccagaaatcg ggttcaacgg
      721 atactgataa aaattccacc taccttccag cccaatcgga gcctgagtgt tataccgccc
      781 ctgctgcggt taatgttacc gataatagtt atagtacagc cctgattcat catcatacta
      841 ctgctgcggc agtctgatga actattgctt ttacggattc tccttattct tccttctatc
      901 cattcgcttt agctttttga gctcttgaag tttgctcttt gtatcaatat taccgttatt
      961 tacagcgaat tccatccaat aa
//
LOCUS       DAZGVQ010000400          968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000400 DAZGVQ010000000
VERSION     DAZGVQ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.404"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..231
                     /locus_tag="ACHLAG_005432"
     CDS             <1..231
                     /locus_tag="ACHLAG_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658466.1"
                     /translation="TEFGYEIDIQPSPESQWISIVTVKTAVGYRHMNVLDDILTPLRI
                     YDASALECILNRYKPAWQTFLYLYENSHEETE"
     gene            231..>968
                     /locus_tag="ACHLAG_005433"
     CDS             231..>968
                     /locus_tag="ACHLAG_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0658467.1"
                     /translation="MYFVDNNSGVTDMPPLAPSQGTQVKWFTEGDGRKGISHIGQDWL
                     NIVQAELLAILTEGKVQPDKAKLNQLVTAIKAIIAANAYSRKNNLKEIADAGAEAQAA
                     ARRHLGLGGLSGKDSLAAADVGALEKSRNFDDVLDKPTARLNLDVYSKGEGDARYLRR
                     DQNGADIPDKGTFINNLGLRETVNKAANALPSDGTAAAANRLATPVNINGVPFDGTRD
                     INITSGMPESTANQRYVTGVRLGAQALS"
BASE COUNT          276 a          213 c          274 g          205 t
ORIGIN      
        1 acagagtttg gttatgaaat agatatccag ccatcaccgg aatcacaatg gattagtatt
       61 gtgacagtga agacggcggt gggataccgt cacatgaatg tactggacga tattcttacg
      121 ccactgcgaa tttatgatgc aagtgcgctg gagtgcattt taaaccgtta taagccagcc
      181 tggcagacgt ttttatatct gtacgaaaac agtcatgagg agacggagta atgtattttg
      241 tcgacaacaa ttcaggtgtg accgatatgc caccactggc cccctctcag ggcacgcagg
      301 ttaaatggtt tactgaaggt gacggacgca aagggatcag ccatatcggt caggactggc
      361 tgaacatcgt gcaggcagag ctgcttgcca ttctgacaga aggaaaggtt cagccggata
      421 aggcaaaact gaaccagctg gtgacagcca ttaaggcaat cattgcagca aacgcataca
      481 gccggaaaaa caaccttaag gagattgcgg atgcaggtgc agaagcacag gctgctgccc
      541 gccgtcatct ggggctggga ggattatcag gaaaagacag tctggctgct gctgatgtgg
      601 gagcactgga gaaatcccgg aattttgacg atgtactgga taaaccgacg gctcgtctga
      661 atcttgatgt ttacagtaag ggagaaggcg atgcgcgtta tctgcgcagg gaccagaatg
      721 gcgcggatat cccggacaaa ggaaccttta tcaataacct cggtttacgg gaaacggtaa
      781 ataaggcggc gaatgccctg ccatcggacg gcaccgctgc ggcagcaaac agactcgcca
      841 ccccagtaaa tattaatggt gttcctttcg acgggacgcg ggatataaac attacgtcag
      901 gaatgccgga atcaacggcc aatcagcgtt atgttacagg ggtaagactg ggagcgcagg
      961 cattaagc
//
LOCUS       DAZGVQ010000401          950 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000401 DAZGVQ010000000
VERSION     DAZGVQ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.405"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..795
                     /gene="gadX"
                     /locus_tag="ACHLAG_005434"
     CDS             <1..795
                     /gene="gadX"
                     /locus_tag="ACHLAG_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFP0658468.1"
                     /translation="AYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVLV
                     SRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQNKNLRYAEML
                     RKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASELLM
                     SPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVF
                     RNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          278 a          181 c          213 g          278 t
ORIGIN      
        1 gcgtacgcaa gacataaata tattctcacc atggttaatg gtgaatatcg ctattttaat
       61 ggcggtgacc tggtttttgc ggatgcaagc caaattcgag tagataagtg tgttgaaaat
      121 tttgtattag tatcaaggga tacgctttca ttatttctgc cgatgctcaa ggaggaggca
      181 ttaaatcttc atgcacataa aaaaatttct tcattactcg ttcatcactg tagcagagat
      241 attcctgttt ttcaggaagt tgcgcaacta tcgcagaata agaatcttcg ctatgcagaa
      301 atgctacgta aaagagcatt aatctttgcg ttgttatctg tttttcttga ggatgagcac
      361 tttataccgc tgcttttgaa cgttttgcaa ccgaacatgc gaacacgagt ttgtacggtt
      421 atcaataata atatcgccca tgagtggaca ctagcccgaa tcgccagcga gctgttgatg
      481 agtccaagcc tgttaaagaa aaaattgcgc gaagaagaga catcatattc acagttgctt
      541 actgagtgta gaatgcaacg tgctttgcaa cttattgtta tacatggctt ttcaattaag
      601 cgagtcgcag tgtcctgtgg atatcacagc gtgtcgtatt tcatttacgt ctttcgaaat
      661 tattatggga tgacgcccac agagtatcag gagcgatcgg cgcagggatt gccgaaccgt
      721 gactcggcgg caagtattgt tgcgcaagga aatttttacg gcactgaccg ttctgcggaa
      781 ggaataagat tatagaattt tactcagaca taaaaaaaac ccggcatagg ggaccgggaa
      841 gaggatagtc tgccgtctcc agactaataa accgttataa cactccctgt tggcacggga
      901 aactttgtgc tctcagtaag ttaaatataa cttttactgg aaataagatc
//
LOCUS       DAZGVQ010000402          943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000402 DAZGVQ010000000
VERSION     DAZGVQ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.406"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(158..388)
                     /gene="ynfN"
                     /locus_tag="ACHLAG_005435"
     CDS             complement(158..388)
                     /gene="ynfN"
                     /locus_tag="ACHLAG_005435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HFP0658469.1"
                     /translation="MKKIIVFFNSEPAVVVPAMTGVNTIMREYPNGEKTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAIRLLS"
     gene            complement(393..458)
                     /gene="ynfR"
                     /locus_tag="ACHLAG_005436"
     CDS             complement(393..458)
                     /gene="ynfR"
                     /locus_tag="ACHLAG_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YnfR"
                     /protein_id="HFP0658470.1"
                     /translation="MKAPSGAFLLGVYSMDTHILR"
     gene            complement(461..649)
                     /gene="ynfQ"
                     /locus_tag="ACHLAG_005437"
     CDS             complement(461..649)
                     /gene="ynfQ"
                     /locus_tag="ACHLAG_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP0658471.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDMNPFGAKCIFCKSMMITF
                     DNISQYLNASRLSLDLKK"
     gene            complement(660..872)
                     /gene="cspI"
                     /locus_tag="ACHLAG_005438"
     CDS             complement(660..872)
                     /gene="cspI"
                     /locus_tag="ACHLAG_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HFP0658472.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT          310 a          188 c          169 g          276 t
ORIGIN      
        1 gtctgttatg actttccagt tacacagtaa gtcgattata tggtgcaaac gtgtaaaaga
       61 taagatgaaa catcgcaata atcaacatac gatagtctaa attttacaca aacagacaaa
      121 gagaattttc ctgaattatc aatgcaatag catcaaatca actcaagagc cttattgctg
      181 cttccagaat ttcttctgaa gtgacatgtc gatccgcggc tacataaatg actttatgat
      241 ctccggtcag agatggaaac cctgcggcca ttacagtaag gtgtgttttt tcgccatttg
      301 gatattcacg catgatggtg ttaactccag tcatcgctgg cactaccact gctggttcag
      361 agttaaaaaa aactatgatt tttttcatga tgttaccgta gtatgtgagt atccatcgaa
      421 tagacaccaa gcaaaaaagc tcccgaagga gccttcattt tcactttttt aaatccaacg
      481 acagacggct ggcatttaag tattgtgaaa tattatcaaa tgtaatcatc attgatttac
      541 aaaagataca ttttgccccg aaaggattca tgtcagaaac atcaaaagat gatgttctat
      601 actgggaacc atgacaacac gggcatctaa agtgaatatg gtttgtaata ttgtctacct
      661 caaagcgcca ctacatgaac agcggcagga cctttaggtc cgttctcaat accaaattca
      721 acttcctgat tctcagttaa tgttttgaaa tcgttgctct gaattgctga gaaatggaca
      781 aacacatctt tgctgccatc tttcggcgtg atgaaaccaa aacctttttc agggttaaac
      841 catttcacta aaccagtcat tttgttagac ataattatta ccttttgaag aaattagccc
      901 ttgggcagaa tggtccgaaa aaaatatcag agagaaaaac caa
//
LOCUS       DAZGVQ010000403          939 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000403 DAZGVQ010000000
VERSION     DAZGVQ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.407"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..530)
                     /locus_tag="ACHLAG_005439"
     CDS             complement(<1..530)
                     /locus_tag="ACHLAG_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658473.1"
                     /translation="MAYFYFKLDRVQTNKYFTKYQQTVLPLRNSILRALLKNTGAAGL
                     RLKPFAMDVISEFYFSGTLPAGWRKRDDVAFIGDGPCFIARPDESCPEGPAIAAMIEA
                     AERELRKRPDFLVWLCEKLGVMRIPSMFNMDSWWTPSLSRDALCVVFKVGAYGREIKG
                     GIPEECQEIKHSEYVAL"
     gene            complement(628..>939)
                     /locus_tag="ACHLAG_005440"
     CDS             complement(628..>939)
                     /locus_tag="ACHLAG_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP0658474.1"
                     /translation="DWCKTNRDELTNGGKTKTANLTTGTVSWRLGNPSCSVSRDVEGV
                     IEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGAESFYVEPFEQDAGLN
                     K"
BASE COUNT          232 a          260 c          199 g          248 t
ORIGIN      
        1 agcgccacat attcagaatg tttaatctcc tggcattctt caggaatacc cccttttatt
       61 tccctgccat aagcgcctac tttaaacacc acgcacaggg catcacggga gagcgacggg
      121 gtccaccagg agtccatgtt aaacatggag gggattctca ttacccccag cttttcacag
      181 agccagacaa ggaaatcagg acgctttctt aactcgcgtt cagcggcttc aatcattgcg
      241 gcaatcgccg gtccttcagg gcatgactca tcaggtctgg caataaagca cggtccgtcc
      301 ccgataaaag ccacatcatc gcgcttacgc cagcccgcag gcagagtacc agaaaaataa
      361 aactcactga ttacgtccat ggcaaacggc tttaagcgca atccggcagc gcctgtattt
      421 ttcagtaacg cccgaagaat actgttgcgt agcggtaaaa cagtctgttg atatttggtg
      481 aaatatttat ttgtctgcac acggtctaat ttgaaataaa aatatgccat atatcctgtt
      541 ccttattttc agtttacagg cgcaggcagt cccctgacgc gagcgccata cttaaaacga
      601 tgtgatattt aaagatttaa tgcggtgtta tttattcagt ccggcgtcct gttcaaaagg
      661 ctcgacataa aaactttcag cgcctttatt caccttaata ccggcaatgc ctttcactgc
      721 atccggctcc gccaggaccg cttccttgtt cacttcctct ttcgtgcgga tgaatcgctc
      781 aagccccata cggcgcagca tttcaatcac accttccaca tcacgactga cgctgcatga
      841 tggattcccc agtcgccatg acaccgttcc ggtggtcaga ttggcagttt tggttttgcc
      901 gccgttcgtc agctcatcac ggttggtttt gcaccagtc
//
LOCUS       DAZGVQ010000404          938 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000404 DAZGVQ010000000
VERSION     DAZGVQ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.408"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(167..>938)
                     /locus_tag="ACHLAG_005441"
     CDS             complement(167..>938)
                     /locus_tag="ACHLAG_005441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0658475.1"
                     /translation="PPATAGSDSTTTESPQPYTPAPQPGAGTQSSPARTTAVAYRADA
                     QEGKLKEQGWTGADGKASVVNDSAAESGKAMRVEKVQGQQTAWKIAHDVPASKAAELL
                     QKGGEIAFRFKIPEGVQLAENRFAMALHWPVSQLPENVVLEGSAGNDMLAAFFIQSDS
                     ANLNLMQHKQQNAKLGTFGAFNHNWHNVVFRFAGNNSISVTPVINGQTQAAFNLAKCP
                     AAGLDNDKLLITDITSNKDTYPVLIEYVTVDINNSNAA"
BASE COUNT          181 a          247 c          237 g          273 t
ORIGIN      
        1 atctgatgaa tgaataacat aatcacctca cacaaatgat tgacataacg atgtctggcg
       61 ttttaaaaaa aatgaataac aataataaac actctccata attcctcttg cgctcccgtc
      121 aggtatcttt tcctctcagt cttgcctgcc gggagcctct tttttcttat gcggcgttgc
      181 tgttattgat atccaccgtc acatactcaa tcagcacggg gtaggtgtcc ttgttactgg
      241 tgatatccgt gatcagcagt ttgtcgttat ccagtcctgc cgccgggcat ttcgccagat
      301 taaaggccgc ctgggtctga ccgttgatga ccggtgtcac gctgatgctg ttatttccgg
      361 caaaccggaa caccacgttg tgccagttat gattaaatgc cccgaaggtt cccagttttg
      421 cattctgttg tttgtgctgc atcagattca ggtttgcgct gtcgctctgg atgaagaagg
      481 cggccagcat atcgtttcct gcgctgcctt ccagaacaac gttttcaggt agctgagaaa
      541 ccggccagtg cagagccatg gcgaagcggt tttctgccag ctgaacacct tccgggattt
      601 tgaaacgaaa ggcaatttcc ccgcctttct gaagtaattc agctgcttta cttgcaggca
      661 catcgtgggc gattttccag gctgtttgtt gcccctggac tttttccact cgcatggctt
      721 taccgctctc agcagcagag tcatttacga cggaggcttt tccatctgcc cctgtccagc
      781 cctgttcttt cagtttccct tcctgtgcgt ctgcgcgata agcgacggcc gttgttcttg
      841 ccggtgatga ctgcgtacct gcgcccggct gaggtgcagg tgtgtagggc tgcgggcttt
      901 ccgtggtggt gctgtctgaa ccggccgtgg cgggaggt
//
LOCUS       DAZGVQ010000405          938 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000405 DAZGVQ010000000
VERSION     DAZGVQ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.409"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(12..230)
                     /locus_tag="ACHLAG_005442"
     CDS             complement(12..230)
                     /locus_tag="ACHLAG_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFP0658476.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(256..630)
                     /gene="tomB"
                     /locus_tag="ACHLAG_005443"
     CDS             complement(256..630)
                     /gene="tomB"
                     /locus_tag="ACHLAG_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFP0658477.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT          268 a          194 c          188 g          288 t
ORIGIN      
        1 cgaattattt attagcgaat aaatttccat actgaggaag ggatcttgtc gtatagttta
       61 ttcatggtca attcggcgag gcggtgatct gcggctgagt aaaataccgc cagttcatta
      121 tctgataatt cgtatttatt tttctcgata acacgctcca gcgtgtcaat tgtctggcaa
      181 cgacgtaaac gcattaaata atcggttttc gttaaaggtt tttcggacat acttctaccc
      241 atggttgtaa taattctaac aagataaact cgcaggattc tctttcgtcg cattgacaaa
      301 acaacggaat agtcgattac ctgacttccg ccatttctga agatcctgca tattaatacc
      361 ataactgctg aacaacataa aggtgtcatc cagatattcg tcgatctgct caatgagctt
      421 attgtcttca ttatacttaa ttttgtaatt aagtgcgaag gtcgcaatat gctcaatcag
      481 ttcattcaac tggaggttga tcgccgaggt tgggtcgtta acccagccat gattgctttc
      541 ttcaaggttt gcaagacagt catgatacag ggtttcacag agaaacttaa gctgtgcgat
      601 atcatgtctt ttgggtgagt attcatccat aacgcgtccc cttcttagcg gttgaactaa
      661 cggacacctt tcgggatgga aaaaacttac tgacctggac ttgccctctc gtttgttagc
      721 ttaactataa gccactcttt gcaggttttc atcgcattat tacgaaaaat tacaattagg
      781 aaggaagcgg gacaggactc actttttcat actagcacta acccgcagca ggtaaaagca
      841 gtttgctcga actactcata acatttcagg attatcgcca gatgaaattg gtgacactaa
      901 taccaggatt gctctgaata tgacgtaata accgagga
//
LOCUS       DAZGVQ010000406          927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000406 DAZGVQ010000000
VERSION     DAZGVQ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.410"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            78..545
                     /locus_tag="ACHLAG_005444"
     CDS             78..545
                     /locus_tag="ACHLAG_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP0658478.1"
                     /translation="MNRVLCVVIIVLLVACGALSLGLNHYRDNAITYKAQRDKKAREL
                     ELANATITDMQVRQRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINATCHGT
                     VREATGTSGVDNATGPRLADTAERDYFTLRERLMLMQKQLEGAQDYIRTQCLN"
     gene            722..>927
                     /locus_tag="ACHLAG_005445"
     CDS             722..>927
                     /locus_tag="ACHLAG_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP0658479.1"
                     /translation="MNYPTIVDGIDFRQLVTAADGEPITDSFQIAKAFGKRHADVLRA
                     LKNCHCSEDFRRAHFCVAEKINELG"
BASE COUNT          219 a          220 c          263 g          225 t
ORIGIN      
        1 actaatgctg gttgctattt ttctttccct gggctggatg atgttgtctt cgtcggggat
       61 gctggggtga gggggatatg aaccgtgttc tgtgtgtggt gattattgtc ctgctggtgg
      121 cctgtggtgc gcttagtctg gggctgaatc attaccgcga taacgccatc acctacaaag
      181 cgcagcgaga taaaaaagcc agagagctgg agctggcaaa cgcaaccatt actgatatgc
      241 aggtgcgcca gcgcgatgtt gctgcgctcg atgcaaaata ctcgagggaa ttagctgatg
      301 cgagagctga aaatgaaact ctgcgtgctg atgttgccgc tggtcgtaag cgcctgcgga
      361 tcaacgccac ctgccacggt accgtgcgtg aagccaccgg cacctccggc gtggataatg
      421 caaccggccc ccgactggca gacaccgctg aacgggatta tttcaccctc agagagcggt
      481 tgatgctgat gcagaagcag ctggaagggg cacaggacta tatccgcact cagtgcctga
      541 attaacagag ccagcttaat cgctggcttt ttcatatctg aatttcaccg cgcatctcac
      601 gcgcatatca catccccgag cctttcagaa agttgagcct gagaactgcc gtatatggtg
      661 gcgaccatct cggggcggct tttctgtgag acaggctcaa ttttctgaaa ggtaaaacgc
      721 gatgaactat ccgacgattg ttgacggtat tgattttcgc caactggtaa cagcggcaga
      781 tggcgaacca ataacagact cttttcagat cgctaaggcg tttggtaagc gtcatgcgga
      841 cgtattgagg gcgctgaaaa attgccattg ctctgaagat ttccggagag cgcatttttg
      901 cgttgccgaa aaaatcaatg agttagg
//
LOCUS       DAZGVQ010000407          925 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000407 DAZGVQ010000000
VERSION     DAZGVQ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.411"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..248
                     /locus_tag="ACHLAG_005446"
     CDS             <1..248
                     /locus_tag="ACHLAG_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP0658480.1"
                     /translation="SSSPDDLRLSLSLLREAMRINPASGVKREIATLERAVRKLADGN
                     QVVPASTTGRKTTTGGKKSSAKKKSAASQKKAARKTN"
     gene            353..847
                     /locus_tag="ACHLAG_005447"
     CDS             353..847
                     /locus_tag="ACHLAG_005447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFP0658481.1"
                     /translation="MSLIARPRVNSADTDTTDTDDGDAMVSAGDFWPAISLHEIRLAS
                     RIPGRTTTTRLLHAATEAVAHVIAELELWKRERMAEGAASLADVPATQVNGESVHVHR
                     YRRAVFALTRAFVLERTRDVDTTDKGDRRADALDMQVEDLWRDARWAISDIRGVTRIY
                     AELV"
     gene            802..>925
                     /locus_tag="ACHLAG_005448"
     CDS             802..>925
                     /locus_tag="ACHLAG_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFP0658482.1"
                     /translation="MRHTRCHPHLCGAGLMKVMALDGDTVDLLCQRHYGTTQGVT"
BASE COUNT          214 a          267 c          282 g          162 t
ORIGIN      
        1 cctcctcctc tcccgatgat ctgcgtctgt cgctgagcct gttacgtgaa gccatgcgga
       61 tcaatccggc ttcgggagtg aagcgtgaaa ttgccacgct ggagcgcgcg gtcaggaaac
      121 tggcagacgg aaatcaggtg gtgcctgcat ccacaacggg caggaaaacc accaccggcg
      181 ggaaaaagag cagcgcaaaa aagaaaagcg ctgccagcca gaaaaaagcc gcgcgcaaaa
      241 caaactgact taacgaaccg cacctcgtcg gcaggcggcg caaccggtga tctgaacagg
      301 cttcgcgttc tttttaccgg ttgcccaccg cctgattttt acggagacag gcatgagtct
      361 gatagccaga ccccgcgtga acagcgctga cacagatacc accgacaccg atgacggcga
      421 cgccatggtg agcgcgggtg acttctggcc ggcgatctca ctgcatgaaa ttcgcctggc
      481 ttcccgcatt ccggggagga caacaacaac ccgtctgtta catgcagcaa cggaagcggt
      541 cgcccatgtg atcgccgaac tggagctctg gaaacgtgag cggatggctg aaggcgcggc
      601 atcactggct gacgttccgg ccacgcaggt gaacggagag agtgtacacg tccaccgcta
      661 ccgcagggcc gttttcgcac tcacccgcgc gtttgtactg gagcgcaccc gggatgtgga
      721 taccaccgat aaaggcgacc gcagggctga cgcgctggac atgcaggttg aggatttgtg
      781 gcgggatgcc cgctgggcaa tatcagacat acgcggtgtc acccgcattt atgcggagct
      841 ggtctgatga aagtaatggc tctggacggt gacaccgtcg atttactctg tcagcgccat
      901 tacggcacca cgcagggggt gactg
//
LOCUS       DAZGVQ010000408          924 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000408 DAZGVQ010000000
VERSION     DAZGVQ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..924
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.412"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            218..293
                     /locus_tag="ACHLAG_005449"
     tRNA            218..293
                     /locus_tag="ACHLAG_005449"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:251..253,aa:Ile,seq:cat)
BASE COUNT          280 a          166 c          196 g          282 t
ORIGIN      
        1 aactatcatt gaatcgatga taactataga tagtgctaat tattaacatt tgcttgttga
       61 ctgcgagtat cgactttgcg aatggaagcg gtgtcgggtt ttatgtcgag tgtaatgacg
      121 agcaaggatg aatgagaacg cataaaaatc tgttatttac atcatatgat catcatgttt
      181 attgcgtggt ttataagtat tgttattgca cactcaaggc cctttagctc agtggttaga
      241 gcaggcgact cataatcgct tggtcgctgg ttcaagtcca gcaagggcca ccaaatcatt
      301 atttacgtat caggaagagt taagggtaca aggggtactc gatggcggtc tatatttcat
      361 gactaacgcc atttcaagga agaagtgcgg tgggtggttt cgaatgaggg ctggtgtttt
      421 ggtacgtggc acgccactgc aatgtattta tccatcagta ctcattcaaa agtacctgga
      481 aaaaatgcct gctatgccaa gcgggcaaat tgcttacatg tggaattaag taattaacga
      541 taaagccaga gtattatcat ttattatccc ccttaattta atcaggttaa ttactcaaat
      601 cactgaacta ttctctgttc gctcactgat gatgagtgag taccacaacc aatgtatgta
      661 gaacaatgcc gtagagtaag gatgaaatgt ctctgccacg ccttaaacat ctactactga
      721 ccagaacacc cgccgcaggc agatgagttt tgtgagcgtt acaggagcgt ggctatgctc
      781 attaaccaac gctattgaga tataaatttc taacactcga aaaacgaatt tcactaataa
      841 attataacgg gtggttttta tcatacagcg aaataacttt ttgtttatta aatttaacat
      901 tcttgtgcta atttaattaa aagt
//
LOCUS       DAZGVQ010000409          922 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000409 DAZGVQ010000000
VERSION     DAZGVQ010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.413"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(65..>922)
                     /gene="xapR"
                     /locus_tag="ACHLAG_005450"
     CDS             complement(65..>922)
                     /gene="xapR"
                     /locus_tag="ACHLAG_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFP0658483.1"
                     /translation="KLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKELENQLGTQL
                     FIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELGVVGTAMWGR
                     MRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPTGFTSLRLHE
                     SAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQVGFSPVVIRE
                     VNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVYETQQVTPAM
                     VKLLAALTQ"
BASE COUNT          235 a          258 c          209 g          220 t
ORIGIN      
        1 tgcgaaattg cctgatgcgc ttcacttagc agacgactat ttccggcaat tcctgtctcc
       61 tcacctactg tgtcaatgca gccaacagct taaccatcgc gggcgtcacc tgctgtgttt
      121 cataaacaat atataaatct gcagggatgc gctgtttgag cggacggaaa atgacacctg
      181 gccagttcat ttgtgcgtag ctgtccgcta tcaatgtgat accaataccc atactgacca
      241 tggcgagtac cgtttgcggt tcattaactt cgcgaataac aaccggtgaa aatcccacct
      301 gctggcaaac tcgctgcaaa aaatcccagt cagtgtacac gggcggcatt gtaacaaaat
      361 actcgtcacg tagcgcttcc agcggaacgg tggaaaatga tgagagatga tgctcttcag
      421 gcatcgccac cagaaacgcc gattcatgca accgtaagct ggtaaaacct gtcggtggtt
      481 ctgtcgccat tcgccagatc ccggcatcga gttcacgtcg ttccagcagg gccatttgca
      541 tcgcgggcat cttttcgcga aaaagaactt caacgttagg attttccctg aggaatcgcc
      601 gcataaccgg gcgcatccgt ccccacatag ccgttcccac tacgccgagt tcaatccgcc
      661 ctgcttctcc ccgacctatt tgttcaaccc gagccaatac attattagca ttcaccagca
      721 atcgacgcga ttcttccatc aagattttgc ccgcgtgtgt cagtacgacg ctgcgcgaat
      781 ggcgaataaa aagctgcgtg ccgagttgat tttccagctc tttaatatga atactgagcg
      841 gaggctgaga catatttaaa cgcgctgctg cgcggccaaa atgcaactct tccgctacgg
      901 caagaaaata acggagcaac tt
//
LOCUS       DAZGVQ010000410          919 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000410 DAZGVQ010000000
VERSION     DAZGVQ010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.414"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(160..291)
                     /locus_tag="ACHLAG_005451"
                     /pseudo
     CDS             complement(160..291)
                     /locus_tag="ACHLAG_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          219 a          215 c          254 g          231 t
ORIGIN      
        1 gggcggctgg tgggtgtggt ggttgttgct ttcccgttgc tgaaaaagaa agcatcaggc
       61 gattagcagg gtatcagtta cccgttgaaa tttttaaata cctcacaatt caggcggttg
      121 actgttgtct ggtttgcggg gagtttgtta aaagaaactg gcatggtgaa tccccctgtg
      181 cggaggggca atcagcaact ggtgttttgt caccgaccct tatcctttct gtgcgggttc
      241 aggtgctgat actgaactca ccgggaggca cccggcacca tgcaatggca catagcgcca
      301 ctctccagcc cctctccgga ggggcttttc tgtgccggat acatcacagt ttctggaacc
      361 ttgggtacta cagtatcagt cagggtgcta tattttcaga tgtgatgaaa gcctgtcagc
      421 aggcagggcg tatcggaaat gacccagtag agaaaacgtt gactcagata ccggtgctga
      481 gttaccggga aaccggcatc acatgaccgc tatccttcca ggcccatccg ctccggtggg
      541 cctttttact gcagaaaaca ggttccccgt taaatgctat gttgctcaca attcagtaag
      601 ttgacagttg cctgtcagac tgggcatttg ttaaaaaaat ttcgcatggt gaatccccct
      661 gagcggaggg gcgactggtg acggtataat ctctgattat caaaacgaga atgacgcggg
      721 tttagtggca ccgggctgaa ctcaccggga ggcacccggc accatgtgca tgatgataca
      781 gatacgcggc tttagcccct ctccggaggg gctttcttgt gggcaaaaaa aagcccgagt
      841 gggttcgggc aacagcatga gatatacatt tttataatcg aatggatttt aaccagaatt
      901 cataaggctg cgcaactgc
//
LOCUS       DAZGVQ010000411          910 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000411 DAZGVQ010000000
VERSION     DAZGVQ010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.415"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..799
                     /gene="yeeO"
                     /locus_tag="ACHLAG_005452"
     CDS             <1..799
                     /gene="yeeO"
                     /locus_tag="ACHLAG_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFP0658484.1"
                     /translation="TISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMG
                     IGIPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTII
                     TGRRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVI
                     LIWLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGV
                     VGVWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPAVSE"
BASE COUNT          188 a          186 c          254 g          282 t
ORIGIN      
        1 aaccatttct cgttatattg gcgcagttgc aattttgtgg gtgctggcga ttggttttaa
       61 tcctgcgcta aggatttcgt taaagagcta ttttaaaccg ctgaatttta gcattatctg
      121 ggaagtcatg gggattggta ttccagcgag tgtcgaatca gtgttattta ccagtggtcg
      181 gttattaacc caaatgttcg ttgccgggat ggggaccagt gttattgccg gaaattttat
      241 cgcgttttca attgcggctc ttatcaactt acccggaagt gcgctcggct ctgcttctac
      301 gatcattaca ggccgaaggt tgggggtagg gcagatagcg caagcagaga ttcagttgcg
      361 gcatgtgttc tggctttcca ctcttggatt aacggccatc gcctggctaa cggctccctt
      421 tgccggggtt atggcatcgt tttacaccca ggatccacag gttaaacatg tcgttgtgat
      481 tctgatttgg ctaaatgctt tatttatgcc tatttggtcc gcctcatggg tgctacccgc
      541 tggatttaaa ggtgctcgcg atgcccgtta cgccatgtgg gtttctatgt tgagcatgtg
      601 gggttgccgg gttgtcgtcg gttatgtgct gggcatcatg cttggctggg gtgtggttgg
      661 tgtctggatg ggaatgtttg ccgactgggc tgtgcgggcc gtgctgtttt actggcgaat
      721 ggttactgga cgttggctgt ggaaataccc tcgatccgaa ccgcaaaagt gtgaaaaaaa
      781 gccagccgtg tcggaataaa cgacaaaatg cagattattt cagcaaacga tttcaaattt
      841 aaaaaacagg ctttgacatt atgggtgggc atcgctaata ttcgcctcgt tctcacgatt
      901 cctctgtagt
//
LOCUS       DAZGVQ010000412          908 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000412 DAZGVQ010000000
VERSION     DAZGVQ010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.416"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(152..823)
                     /gene="queE"
                     /locus_tag="ACHLAG_005453"
     CDS             complement(152..823)
                     /gene="queE"
                     /locus_tag="ACHLAG_005453"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFP0658485.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          235 a          228 c          205 g          240 t
ORIGIN      
        1 cagcgaaaaa ttaatgccac agaatttgta gagaatgcta atgattagcc ctggatgggt
       61 aaaatggagg agttttcaga ggcgacaaac aatataaatg agtagaagtc tttacaggtt
      121 ccacccattt ataacgctta taaatgttta atcaggcaat atttagatat ttatgtgttt
      181 gcatcgacaa acgccaatta cgcgcaatgc aggtttcaat gcacaaacgt gtggcatcat
      241 ccttttggct aatcggctgc agtgcaatga ctcgcggttt atcatcggtc agcgttgcca
      301 atagttcatc cagtgcttca atatcgcgta cgcgccccac tggatgcttg atttcgttgg
      361 ctcgctccag tgcctgtgac aacacgtcat agccgccgcg catgttcagc tttggcgata
      421 cggtaaccca ggtattcggt gtgcagcgta cttcatgagt tccactggtt tcgatctggc
      481 agctaaaacc gttcttttcg agcaggtcag tcagtggcag caaatcatga atgcaaggct
      541 caccacccgt aatcaccaca tgccgcgcgg tatatccctg ccgaccaatg acagccagca
      601 aatcttcact gctcgccgcc ccccacttat cactctcttt ggtcttcgcc agaatgctga
      661 aaagggagac ttcccgatcc tcaagctttt cccaggtgtg tttggtgtcg caccaggcac
      721 agccaaccgg gcatccctgt aaacgaataa aaatggcggg gacgccggta aagtaaccct
      781 caccttgcag ggtctggaac atctcgttaa tcgggtactg catagcattc tctgtgaagt
      841 ggataattgt taattattgc agatcctgcc acaacaatca tgtcttatta acattctgtt
      901 acaggcag
//
LOCUS       DAZGVQ010000413          904 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000413 DAZGVQ010000000
VERSION     DAZGVQ010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.417"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..198
                     /locus_tag="ACHLAG_005454"
     CDS             <1..198
                     /locus_tag="ACHLAG_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HFP0658486.1"
                     /translation="LLIVNNKMLNLPIRKLFPTRIASSYGMNFEFMPELKWGFGYPGA
                     IIFMILAGLTPYLYFKRKNWL"
BASE COUNT          279 a          135 c          175 g          315 t
ORIGIN      
        1 ttgctcattg ttaataacaa aatgttgaat ttaccgatta ggaaactttt tcctacgcgg
       61 attgcctcca gctatggcat gaactttgag tttatgccag aactgaagtg gggcttcggc
      121 taccctggcg cgattatctt tatgatcctc gcgggcctga caccgtatct gtactttaag
      181 cgtaagaact ggctgtaact atacgatgtc gctgtgagta agttagtgtg ctgacagcga
      241 cattttagcc gagggaatcg cagataacat aattgttgtg aataatatcc atgccacgtt
      301 gagttggacg cttaatgact cacatctcac cccttcaatt gctgcagaga taatagaaaa
      361 aagtgtcaag cccaatgaaa tcaatacaaa gtagattgcc ttacatccag tatctcttat
      421 tctttttgct ccaatattga tatttacata ggtattatta ataaccagaa taaaactcaa
      481 agccaggttg atatcattca aagttattaa tattgaccat gatgttaatg tatagattat
      541 gaactgaaag cgtgataaac gctcaagcct ggatgggtag aatatatttc tgatggatga
      601 tttgaacatt tttacattgt tttatatata attgttttga atatatatta cctgaattat
      661 gtggtttgca tatatatatg caatatagca gtctttgcat tgataactat acagtagtaa
      721 tttagagtgt gtaagttttt attgatgggg tatattttaa ttaaatataa ttgaaatgta
      781 tttttattct ataaacaata tcttgggtga atggattcta tatgggaaat ggtaactttt
      841 ggcagttgtt cttgggtaaa gaaccttaca gcaaaaaatc tgattttgtt aaggcatggt
      901 tatt
//
LOCUS       DAZGVQ010000414          890 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000414 DAZGVQ010000000
VERSION     DAZGVQ010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.418"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            17..649
                     /locus_tag="ACHLAG_005455"
                     /pseudo
     CDS             17..649
                     /locus_tag="ACHLAG_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
     gene            complement(668..890)
                     /locus_tag="ACHLAG_005456"
                     /pseudo
     CDS             complement(668..890)
                     /locus_tag="ACHLAG_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843029.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
BASE COUNT          296 a          139 c          177 g          278 t
ORIGIN      
        1 cactcaggat gaatatatgg gaaaaattaa aattgtagtt tcagatcagc agccgtttat
       61 gattgatggg ataattggat ttctcggaca ttatcctgat ttatataagg ttgttggggg
      121 ctataaagat ctgaagaaag ctatagcgga gtgtaacaaa tctacagcct aaatatttat
      181 tttaggagaa tttgcaggtg gaatgatggg gtccgaactc gtaaaatggg ttaaatcgca
      241 taagatagat gctcatatta ttacatttgt agcaaagatg ccgtatattg attcaataaa
      301 attgcttgaa gcaggtgcga aaggatgcgt atggaaaacc agtcacccgg cgaaactaaa
      361 tcgtgctatt gattcgatta gtaatggtta cacttatttt gatagtgtac atatggattg
      421 tgaaaaaata tcttccaggt attcctctga taatcaactt acaaatcgtg agtcagaaat
      481 attacaactc atagctgatg gtaaaacaaa caaagaaatc gctaatttct tgcagttaag
      541 cagaaaaaca gtcgaaactc atagactcaa tatcatgaag aaattagatg ttcacagtgg
      601 tatcgagttg atcaaaacag ctttacgtat gggtgtttgc actatataaa aaaaagaggc
      661 tttaggctca ttttgctgaa gccttacgat tgtctagttc attaagcctg gtatatacaa
      721 tatatatttt ggggcatatt gctgtctctt tttttggtta tccattgcaa aacccgcatt
      781 ataagtcccc aggcattgcc acgttactcc acaacgtgaa aaatggttat ataaaatctc
      841 ggttcctatt ttaatgttca gacaagggtt atctaacaaa tctcgttcat
//
LOCUS       DAZGVQ010000415          888 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000415 DAZGVQ010000000
VERSION     DAZGVQ010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.419"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            146..>888
                     /locus_tag="ACHLAG_005457"
     CDS             146..>888
                     /locus_tag="ACHLAG_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFP0658487.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIG"
BASE COUNT          241 a          165 c          227 g          255 t
ORIGIN      
        1 aagcattagc aaattagcca aattgacacc ttcccacctc caactaacac tcaatacaga
       61 cctctgacaa tttaataagt ttcattttta atgtctgacg actcatctct ttcgtggctg
      121 tattgtttgt atatggagcg tttatatgaa tagaatctat cgcgtgatat ggaattgcac
      181 tctacaggta tttcaggcct gctcggaatt aactcgcagg gcaggtaaaa catcgacggt
      241 taatttgcgt aaatcctctg gactgacaac gaaattcagt agattgacgc tgggtgtttt
      301 gctggcacta agcggttcag cgtctggtgc aagtctggaa gttgataatg atcagattac
      361 caatattgat actgatgttg cttatgatgc ctacctggtt ggctggtatg gcactggagt
      421 gcttaatatt ttggctggcg gtaatgcctc cttaaccact attactacca gcgtcattgg
      481 cgctaatgag gactcagagg gcaccgttaa tgttttgggt ggcacctggc gattgtatga
      541 tagcggaaat aatgcaaggc ctttaaatgt gggtcaatcc ggaacgggga cgctgaatat
      601 taaacagaag ggtcacgtcg atggaggcta tttaagatta ggttcttcga caggaggcgt
      661 cgggacggtc aatgttgagg gagaggactc tgttctgacg accgaattat tcgaaatagg
      721 gagttacggt acgggctcat taaatattac ggataagggt tacgtcacga gttcaatcgt
      781 cgccatttta ggctatcagg cgggcagtaa tggccaggtt gtcgttgaaa agggtggcga
      841 gtggctaata aaaaataacg attcctcaat tgaatttcaa attggtaa
//
LOCUS       DAZGVQ010000416          884 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000416 DAZGVQ010000000
VERSION     DAZGVQ010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.420"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>884
                     /locus_tag="ACHLAG_005458"
     CDS             <1..>884
                     /locus_tag="ACHLAG_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP0658488.1"
                     /translation="FRINAPAAPAGVELTPGYFQITAVPRLAVYDPTVQFEFWFSEAK
                     IADAAQVETSARYLGTGSQWSVSGSQIKPGKVFWFYVRSVNLVGKSAFVEASGRASDD
                     AAGYLEFFREKIGKTHLAEALWAEIDNSKLKDEMAEMQTTITETRNEITQTVSKTLED
                     QNATIQQIQRVQTDTNNDLNSMWAVKLQQMQDGRRYIAGIGAGIENTPDGMQSQILLA
                     ADRVAFINPENGDTTPALVTQGGQTFINEALIKVLTAPTITSGGNPPAFSLTPDGRLT
                     ARNADISGNISASSGTLN"
BASE COUNT          221 a          225 c          267 g          171 t
ORIGIN      
        1 ttccggatta acgcaccggc agcgcctgcc ggtgttgaac tgacgccggg gtattttcag
       61 ataacggcgg taccgcgtct tgcggtgtat gacccgacgg tgcagtttga gttctggttt
      121 tcggaggcaa aaattgcaga cgccgcacag gtggaaacct ctgcccgtta tctggggaca
      181 ggcagccagt ggagcgtctc cggcagccag attaaacccg ggaaagtttt ctggttttac
      241 gtgcgcagcg tcaatctggt gggtaaatcc gcgtttgttg aagccagtgg acgggccagt
      301 gatgatgctg cgggctatct ggagtttttc cgggaaaaga taggaaaaac acatctggca
      361 gaggcgttgt gggcagagat tgacaacagc aagctgaagg acgagatggc ggaaatgcag
      421 accaccatca cagaaacccg caatgaaatc acgcagacgg tcagtaaaac gctggaggac
      481 cagaacgcca ccatacagca aatccagcgt gtgcagacgg acacaaataa tgacctgaac
      541 agcatgtggg cggtgaagct acagcagatg caggatggtc gtcgctatat tgccggtatc
      601 ggtgccggta ttgagaacac ccctgacggg atgcagagcc agatactgct ggcagcggac
      661 cgtgtggcat tcattaatcc ggagaatggt gataccacac ccgcactggt cacgcagggg
      721 ggacagacgt ttatcaatga ggccctgata aaggttctta ccgcccccac cattaccagc
      781 ggcggcaatc ccccggcatt ctccctgacg ccggacggca ggctgacggc ccgtaatgcc
      841 gatatcagcg ggaatatcag tgccagttcc ggtacgctga ataa
//
LOCUS       DAZGVQ010000417          883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000417 DAZGVQ010000000
VERSION     DAZGVQ010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.421"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>883
                     /locus_tag="ACHLAG_005459"
     CDS             <1..>883
                     /locus_tag="ACHLAG_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP0658489.1"
                     /translation="AGRPEMRYRYDDTGRVVEQLNPAGLSYRYQYEQDRITVTDSLNR
                     REVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGLNVVSGDI
                     TDITTPDGRETKFYYNDGNQLTAVVYPDGLESRREYDEPGRLVSETSRSGETVRYRYD
                     DAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTAVHREEGI
                     SLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQYDAWGKAVSTT
                     QGGLTRSMEYDAAGRVI"
BASE COUNT          205 a          244 c          316 g          118 t
ORIGIN      
        1 acgcgggaag gccggagatg cgctaccgct acgacgatac cgggcgggtg gtggagcagc
       61 tgaacccggc aggcctgagt taccgctacc agtatgagca ggaccgcatc accgtcacgg
      121 acagcctgaa ccggcgtgag gtgctgcata cagaaggcgg ggccgggctg aagcgggtgg
      181 tgaaaaaaga actggcggac ggcagcgtca cgcacagcgg gtatgacgcg gcaggaaggc
      241 tcacggcgca gacggacgcg gcgggacggc ggacagagta cggtctgaat gtggtgtccg
      301 gcgatatcac ggacatcacc acaccggacg ggcgggagac gaaattttac tataacgacg
      361 ggaaccagct gacggcggtg gtgtacccgg acgggctgga gagccgccgg gaatatgatg
      421 aaccgggcag gctggtatcg gagacatcgc gcagcgggga gacagtacgc taccgctacg
      481 atgacgcgca cagtgagtta ccggcgacga caacggatgc gacgggcagc acccggcaga
      541 tgacgtggag ccgctacggg cagttgctgg cgttcactga ctgctcgggc taccagaccc
      601 gctatgaata cgaccgcttc ggccagatga cggcggttca ccgcgaggaa ggtatcagcc
      661 tttaccgcca ctatgacaac cgtggccggt taacctcggt gaaagacgca cagggccgtg
      721 aaacgcagta tgagtacaac gccgcaggcg acctgactgc cgttatcacc ccggacggca
      781 accggagcga gacacagtac gatgcgtggg gaaaggcggt cagcaccacg cagggcgggc
      841 tgacgcgcag tatggagtac gatgctgccg gacgtgtcat cag
//
LOCUS       DAZGVQ010000418          873 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000418 DAZGVQ010000000
VERSION     DAZGVQ010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.422"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            274..795
                     /locus_tag="ACHLAG_005460"
     CDS             274..795
                     /locus_tag="ACHLAG_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP0658490.1"
                     /translation="MQLINVKRYYPENTLYGNDVQYFQSEDGKDFYESLPLFTKKYKL
                     CITPDSGIICSISQDASALYPAGFSVVEVDELPEGADISGNWKFDNGVISRIPVNYAR
                     KLEAMRQLYLNQAYEKINDWRTELQLGTISDEDRAALTRWMAYISQVKKMEPSAIKTE
                     AEFDAIKWPEQPE"
BASE COUNT          260 a          172 c          192 g          249 t
ORIGIN      
        1 ctggaatata attactccct gtcatcagga aatgtgatta ccggttttaa gactgacgga
       61 gactgggaaa tgcgtggtgg tgatgaccgt gtttattaca gacagataca atattgtatt
      121 aatgggaatt gggtatccgc tgcaagtatt taattaacgg ctcatggcgt acggcaggca
      181 gcgcctgata tatgtgttat ctcagacaat ccctgtccgt atatagcgat agtattattc
      241 ctgtttttat cattgattca catggtgatg tttatgcaac tgattaatgt taaacgatat
      301 tacccggaaa acaccctcta tggtaatgat gtacagtatt ttcagagtga agatggtaag
      361 gatttctacg agtccctgcc attattcacc aaaaaatata aactttgtat aacacctgac
      421 agcggcatta tctgctcaat atcacaggac gcttcggcgc tgtatcctgc ggggttctct
      481 gtggttgaag tcgacgaact ccctgaaggt gctgatattt cgggtaactg gaagtttgat
      541 aatggcgtga tctcccgtat tccggttaac tacgcaagaa aactggaagc aatgcgccag
      601 ttatatctta atcaggcata tgaaaaaatt aatgactgga gaacagaatt acagctgggc
      661 accatcagtg atgaagacag agccgcactt acccgatgga tggcatacat cagtcaggtg
      721 aaaaaaatgg aaccatccgc cattaaaact gaagctgaat ttgatgccat taaatggccc
      781 gaacagcctg aataacttaa agggtgctta tcgcaccctt tttctttcat cagacagatg
      841 aaactgtacg ccatgcatta ctgatatata tct
//
LOCUS       DAZGVQ010000419          849 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000419 DAZGVQ010000000
VERSION     DAZGVQ010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.423"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..190
                     /locus_tag="ACHLAG_005461"
     CDS             <1..190
                     /locus_tag="ACHLAG_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="required for invasion of epithelial cells; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFP0658491.1"
                     /translation="VVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLALISVATCW
                     LLWKYRAILTNLVRLKIK"
     gene            206..786
                     /locus_tag="ACHLAG_005462"
                     /pseudo
     CDS             206..786
                     /locus_tag="ACHLAG_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
BASE COUNT          275 a          141 c          146 g          287 t
ORIGIN      
        1 tgttgtattg tcaaaacgaa gagacattat tgagcaagca ccaacatatg aaattagcga
       61 acctgttttt gcttatgata aggctatgcc ggtttctatc ttacttgcat taatatccgt
      121 agcgacttgc tggctgttat ggaagtatag ggccattcta actaatttag tcaggttgaa
      181 aattaagtag acatatgaga gcgttatgaa tcttgcccta agaaagatta tatatgatcc
      241 aattagttat attcatcctc aacgagtaag cttgaataat acacctatta acaatccggt
      301 gcttagatct attaccaatg aaatgattgt tctgcagtac aatctttcag ttgaacattt
      361 taatctgaat agttcactta tttactacat aaacaattgg aatttatttc cattgttctg
      421 tttattcagt ggttatcact tttatcgaga aaggtttgca gaacgcggat ttttttataa
      481 ggttcctgct gtgctaaggg attatttatc agctataccg gtgaaaataa atgaaaaggc
      541 acgatacaaa cccggcatcg ctagctatca caatatcatc acatgtggtt tttcaacgtt
      601 gtcaccctat ataaggcaac aaccattggc aatgcagcaa cgatttaatt tattatttcc
      661 tgattttgtt gatcacatac aattgccgtt accacttgca tcaactcttt tagagagaat
      721 tacattttat gcgaaaaaaa tagagatgag cttgataaaa tctcctgcaa atggtgttgt
      781 gattaaaaga aaaatttctg atggattgaa ataaatagtt tctcttaaag aaagtatcct
      841 tcttgaaac
//
LOCUS       DAZGVQ010000420          834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000420 DAZGVQ010000000
VERSION     DAZGVQ010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.424"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..579
                     /locus_tag="ACHLAG_005463"
     CDS             <1..579
                     /locus_tag="ACHLAG_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFP0658492.1"
                     /translation="GPAGSVASVPDASTSQKGVVQLSSDTNSDDETKAATPKAVKAVM
                     AEVQAAKTKAEEAATRAAVPGPKGDRGEPGAPGAVGPAGPQGPTGEPGPQGPTGRLVP
                     GEIYSIGTYIIAALAPYITDIGRTYQPGETVSGSRLKRCAIIKDENGNYMKANTDGID
                     GILSSVPGSWMVCNEITSTNDNEGIGLFQRAY"
     gene            599..>834
                     /locus_tag="ACHLAG_005464"
     CDS             599..>834
                     /locus_tag="ACHLAG_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFP0658493.1"
                     /translation="MWKKISNPQWADKDHTAVNCMVKFEHIEQAVPFTATASDTEAYG
                     RDIYAACLRGEAGEIAEYVQPSISPEKARELKTAG"
BASE COUNT          253 a          206 c          242 g          133 t
ORIGIN      
        1 ggtcccgcag gcagtgtcgc cagtgttccg gatgccagca catcacagaa aggtgttgta
       61 cagctgagca gcgataccaa cagcgatgat gaaacaaaag cggcaacccc aaaggctgtg
      121 aaagcggtaa tggcagaagt gcaggcggca aaaacgaagg cagaagaggc agcaacccgg
      181 gcagcagtcc ccggaccgaa gggggacagg ggggaacctg gcgcacctgg tgcagtgggg
      241 cccgcaggcc cacagggccc gacaggtgaa cccggaccac aggggccaac cggaaggctg
      301 gtacccgggg aaatttacag catcggaaca tacattattg ccgcactagc cccgtatata
      361 accgacatcg gcagaaccta tcagccagga gaaactgtat cgggttcacg ccttaagcga
      421 tgcgccataa tcaaagacga aaacggaaac tatatgaaag ctaacaccga tggcattgac
      481 ggcatactat cctcagttcc gggctcatgg atggtgtgta atgaaattac gtcgacaaat
      541 gacaatgaag gcatcgggct gttccagcgc gcatactgac agacaggaga tgacatgaat
      601 gtggaaaaaa ataagcaatc cgcaatgggc agataaagat cacactgctg tgaattgcat
      661 ggtgaagttt gagcacattg agcaggcggt tcctttcact gcaacagcca gcgataccga
      721 agcatacggg cgggatattt atgccgcttg tctgagagga gaggccggtg aaattgcaga
      781 atatgtacag ccatccatca gcccggaaaa agccagagaa ctcaaaaccg ccgg
//
LOCUS       DAZGVQ010000421          829 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000421 DAZGVQ010000000
VERSION     DAZGVQ010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.425"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..767
                     /gene="ydeO"
                     /locus_tag="ACHLAG_005465"
     CDS             6..767
                     /gene="ydeO"
                     /locus_tag="ACHLAG_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFP0658494.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
BASE COUNT          252 a          165 c          165 g          247 t
ORIGIN      
        1 ataaaatgtc gctcgtttgt tctgttatat ttattcatca tgccttcaac gctaacattt
       61 tagataaaga ttacgccttc tctgacggcg agatcctgat ggtagataac gctgttcgta
      121 cgcattttga accttatgag cggcatttta aagagatcgg atttactgaa aataccatta
      181 aaaaatatct acaatgcact aacatccaga cagtgacggt gcctgttcct gcgaagtttt
      241 tacgtgcttc aaatgtaccg actggattgc ttaatgaaat gattgcttat ctcaactcgg
      301 aagaacgcaa tcatcataat ttttcagaac ttttgctttt ttcttgcctg tctatttttg
      361 ccgcatgcaa aggtttcatt acactattaa ctaacggtgt gctatccgtt tccggaaaag
      421 tgagaaatat tgtcaacatg aagctggcgc acccatggaa gctgaaagat atttgtgact
      481 gcctgtacat cagtgaaagc ctgttgaaga aaaaactaaa gcaagagcaa acgacattct
      541 cacagattct attagatgcc agaatgcagc acgcaaaaaa tttgatacgc gtagaaggtt
      601 cagtcaataa aattgccgaa caatgtggtt atgccagtac atcttatttt atttatgcgt
      661 tccgcaaaca tttcggcaac agtccgaaga gagtttctaa ggagtaccgt tgtcaaagtc
      721 acacgggtat gaatacgggc aacacgatga gtgctttagc tatttgatta tttgctaacg
      781 agtagtcaac cacgcacgct acgtaagaat tatatggggc agccattcc
//
LOCUS       DAZGVQ010000422          823 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000422 DAZGVQ010000000
VERSION     DAZGVQ010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.426"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..492
                     /locus_tag="ACHLAG_005466"
     CDS             <1..492
                     /locus_tag="ACHLAG_005466"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFP0658495.1"
                     /translation="TGKLGKQFEKFIDPVDSERNGGTVNAATAVWWAGQSVEARACLR
                     NADGTELAAVTEFLAFISRNMHDESPGNSLNIWCKGASFDFPILKSAITRTAGEKSIP
                     WCYWNERCMRPLIAMAESTGWKMPGRSGKETIAHTALGDAVYQAKVVSEIWQRFTTPF
                     LNI"
BASE COUNT          225 a          220 c          222 g          156 t
ORIGIN      
        1 accggaaagc tgggcaagca gtttgagaaa ttcatcgacc cggtggattc agaacgcaac
       61 ggcggaaccg tcaatgcagc caccgctgta tggtgggccg gacaatccgt ggaagccaga
      121 gcctgcctgc gcaacgcaga tggaacagag ctggcagcag tgaccgaatt tctggcgttc
      181 atttcacgca atatgcatga cgaaagcccg ggcaattcac tgaacatatg gtgtaagggc
      241 gcatccttcg atttcccgat cctgaaatcc gccataacac gcacagccgg agaaaaaagc
      301 atcccctggt gctactggaa cgagcgctgt atgcgcccgc ttatcgccat ggctgaatca
      361 actggatgga aaatgccagg cagatcaggc aaagagacca ttgctcacac cgcgctgggc
      421 gacgccgttt accaggcaaa ggtcgtgtca gaaatctggc agcgcttcac aacaccattc
      481 ctgaacatat gaggtgaagc catggtcgcc atcctgaatg tgtgttgctg cccccccctg
      541 aacgtggaaa gaaaagcccg cgcatcgctg cgtttattcc gcgacggcgt gcggaatttt
      601 tcacgcataa aaccccacga ctacctggta ataagaatcg gttttcgctg gcgattgctg
      661 agcaaaaaca acggggcgtc atgggattta ttaacgcacg aaacctataa caaagaggcc
      721 gcgttatgag caaagaactg ttatccggca cgccgcttaa atgggtgggc ggaaaaagtc
      781 gcatcatgaa cacactgaag gcgcatctgc cagaagcaga ctg
//
LOCUS       DAZGVQ010000423          816 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000423 DAZGVQ010000000
VERSION     DAZGVQ010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.427"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..750
                     /locus_tag="ACHLAG_005467"
     CDS             <1..750
                     /locus_tag="ACHLAG_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP0658496.1"
                     /translation="STLLQIDTLIRQLAEISAMTESIGGKTARDWAMKQDFRCGCWLM
                     EKTETAMKAITRNLDREIWRDLMQRSGMLSLMDAQARDTWYRSLEYDNFPEISEANIL
                     STFEQLHQNKDEVFERGVINVFRGLSWNYKTNSPCKFGSKIIVNNLVRWDRWGLHLIT
                     GQQADRLADLERMLHLFSGKPIPDSRENIIIRLDDHIRSVQGKASYEDEMFNIRYFKK
                     GSAHITFRKPELVDRLNDIIARHYPEMLPSL"
BASE COUNT          239 a          191 c          203 g          183 t
ORIGIN      
        1 agtacactgc tgcaaatcga cacacttatc cggcaactgg ctgaaatctc agcaatgacg
       61 gaaagcattg gcggcaaaac cgcccgggac tgggccatga aacaggattt tcgctgcggc
      121 tgctggctga tggagaaaac ggaaaccgca atgaaagcta tcacccgcaa ccttgaccgc
      181 gaaatctggc gtgacctgat gcaacgttca gggatgctgt cattaatgga tgcacaggcc
      241 cgtgatacat ggtaccggtc actggagtac gataattttc cggaaatcag cgaggcgaat
      301 atcctgagca catttgaaca actgcaccag aacaaagatg aagtgtttga gcgaggggtg
      361 atcaacgtct tcagagggct gagctggaat tacaaaacca actccccctg taaatttggc
      421 agtaaaatta tcgtcaacaa tctggtgagg tgggaccgat ggggattgca tcttatcacc
      481 gggcaacagg cagatcgact cgccgacctg gaaagaatgc tgcatctgtt cagcggcaaa
      541 ccgatccccg acagccggga aaatatcatc attcggctgg atgatcacat ccgctccgtt
      601 cagggcaaag cgagctatga agatgagatg ttcaacatca gatactttaa gaagggctcc
      661 gcacacatca cgttcaggaa accagaactg gttgatcggc taaatgatat tattgccagg
      721 cactatccgg aaatgttacc ttcattataa agctctaaga atgaatgcaa ttatgtattt
      781 tagcttcatt ccctcacgca aatttatttg ggggtt
//
LOCUS       DAZGVQ010000424          805 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000424 DAZGVQ010000000
VERSION     DAZGVQ010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.428"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            190..792
                     /gene="fimB"
                     /locus_tag="ACHLAG_005468"
     CDS             190..792
                     /gene="fimB"
                     /locus_tag="ACHLAG_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFP0658497.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          229 a          154 c          180 g          242 t
ORIGIN      
        1 tggcgtttgt atggcaacgt tattataatt aactgttgct actccattta agttcactca
       61 gaagaactgg tccacttacg ttagttatta agcaaacgtt cgcttttata aacataatca
      121 ggataaaaat gttggattat tgctaaccca gcacagctag tgcgcgtctg taattataag
      181 ggaaaaacga tgaagaataa ggctgataac aaaaaaagga acttcctgac ccatagtgaa
      241 atcgaatcac tccttaaagc agcaaatacc gggcctcatg cagcacgtaa ttattgtctg
      301 actttgcttt gttttattca tggtttccgg gcgagtgaaa tttgtcgatt gaggatttcg
      361 gatattgatc ttaaggcaaa gtgtatatat atccatcgat taaaaaaagg cttttcaaca
      421 acgcatccgc tattgaacaa agaagttcag gctttaaaaa actggttgag tatccgtact
      481 tcgtacccgc atgctgagag cgagtgggta tttttatctc gtaagggaaa tccgctttct
      541 cggcaacagt tttaccatat tatctcgact tccggtggta atgccgggtt gtcactggag
      601 attcatccgc acatgttacg ccattcgtgt ggttttgctt tggcgaatat gggaatagat
      661 acgcgactta tccaggatta tcttgggcat cgcaatattc gtcatactgt ctggtatacc
      721 gccagcaatg cagggcgttt ttacggcatc tgggatagag ccagaggacg acagcgtcac
      781 gctgttttat agagatagtt gagat
//
LOCUS       DAZGVQ010000425          802 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000425 DAZGVQ010000000
VERSION     DAZGVQ010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..802
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.429"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            40..>802
                     /locus_tag="ACHLAG_005469"
     CDS             40..>802
                     /locus_tag="ACHLAG_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658498.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVK"
BASE COUNT          262 a          166 c          178 g          196 t
ORIGIN      
        1 atattctaat cattatgaca ggcgagggag tgtccaatta tgaattcaaa aaagctttgt
       61 tgcatatgtg tgttattctc gctgcttgca ggatgtgcct ctgaatcttc tattgatgaa
      121 aagaagaaaa aagctcaagt cacacaaagt aatattaata aaaacactcc ccagcaactg
      181 acagacaaag atttattcgg taatgaaacc actctggccg tatccgaaga ggatattcaa
      241 gctgcgcttg atggagatga gttccgcgtt ccccttaatt ctcctgtaat acttgttcaa
      301 tccggcaacc gcgcaccgga aaccattatg caggaggaga tgcgaaaata ttatactgtt
      361 tccacattct ctggtatccc ggacaggcaa aagcctctga cttgtaacaa aaacaaagat
      421 aaaaatgaga acgaggatgt tgccagtgct gagaatatga actggatgca ggcactgcgt
      481 tttgtggctg caaaaggaca tcagaaagcg attattgttt accaggatac attgcagaca
      541 ggaaaatatg actctgcgct gaaatcaaca gtatggtccg actataaaaa tgaaaaactc
      601 accgacgcta tctccctgcg ctacctggta cgtttcacgc tggtggatgt ggcaacaggt
      661 gagtgggcta cctggtcgcc ggtgaattat gaatataaag tgcttccacc attgcccgac
      721 aagaatgaag ccagtacgac tgatatgaca gagcagcaaa tcatgcaact taagcaaaag
      781 acctataaag cgatggtaaa ag
//
LOCUS       DAZGVQ010000426          791 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000426 DAZGVQ010000000
VERSION     DAZGVQ010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.430"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..376
                     /gene="mchS3"
                     /locus_tag="ACHLAG_005470"
     CDS             <1..376
                     /gene="mchS3"
                     /locus_tag="ACHLAG_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MchS3 family protein"
                     /protein_id="HFP0658499.1"
                     /translation="SSDPPPLQQSLEKTTYFSIGMNGFIGYQSEGEKLYTHILTLDNP
                     EEIFKNIIKNRKSTKESKIYAACGLYYLNEENIESLFNENDKQEYVSVLRGDILTKIK
                     LNDILNSVIINGCNTKLISEHK"
BASE COUNT          282 a          126 c          151 g          232 t
ORIGIN      
        1 ttcatcagat ccacctcccc ttcaacaatc gttagaaaaa acaacctatt tttctatagg
       61 tatgaatggg tttataggct atcagagcga aggggaaaaa ttatacacac acattcttac
      121 attagataat cccgaagaga tatttaaaaa tataataaaa aatagaaagt caactaagga
      181 gtctaaaatt tatgctgctt gtgggctata ttatttaaac gaagaaaata tagagtcatt
      241 gtttaatgaa aatgataaac aagaatatgt gtctgtctta agaggggata ttttaacaaa
      301 aataaaactg aatgatattc tgaattctgt gataataaat ggttgcaaca ccaaattaat
      361 atctgaacat aaatgagcag gaggttttta aaatgagaga aatatcagat cacatgcttg
      421 atttcgtgaa aggagggatg aatcttaatg gattacctag ttctactaat gtaatagatc
      481 tacgaggaaa agatatggga acatatattg atgctaatgg agcatgctgg gctccgggta
      541 ctccatccat catcatgtat ccggggggaa gtggaccttc ttatagtatg agtagttcca
      601 catccagtgc aaacagcggc agttaaataa ataccctccg gcatggccgg agggtattta
      661 tgatgtgcct gtaagactca acttctgtcg tctctttaca aaaggcatat gatctgtcat
      721 tttgtaccag acatcttaac gagagccttt tcatatatta cttacatacg tgaaatctac
      781 agtataaagc a
//
LOCUS       DAZGVQ010000427          790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000427 DAZGVQ010000000
VERSION     DAZGVQ010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.431"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>790
                     /locus_tag="ACHLAG_005471"
     CDS             <1..>790
                     /locus_tag="ACHLAG_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0658500.1"
                     /translation="TRTITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLERN
                     GNWVTEKDVTINGKTTSQYLTSVILNNLPERPFNIRMVRVTADSTTDQLQNRTLWSSY
                     TEIIDVKQCYPNTAIVGLQVDAEQFGGQQMVVNYHIRGRIIQVPSNYDPEKRTYSGIW
                     DGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDGFGG
                     TEPRMTFNAYLSQQRKVWDVLGDFCSAMRCMPVWNGQTLTFVQDR"
BASE COUNT          173 a          214 c          250 g          153 t
ORIGIN      
        1 tgacgcgcac catcacgtca gcgaacattg accgtctgcg ggtcaccttc ggggtgcagt
       61 cactggtgga gaccacctca aagggtgacc gtaatccctc ttctgtccgg ctgctgattc
      121 agcttgagcg taacggtaac tgggtgacgg agaaggatgt caccattaac ggcaagacca
      181 cctcgcagta cctgacgtcg gtgattctga ataatctccc tgagcgcccc tttaatatcc
      241 ggatggtcag ggtgacggcg gacagcacca cggaccagct gcagaacaga acgctgtggt
      301 cgtcatacac cgaaatcatc gatgtgaaac agtgctaccc gaacacggcc attgtggggc
      361 tgcaggtgga tgcggagcag ttcggtggcc agcagatggt ggtgaactac catatccgcg
      421 gccgcatcat tcaggtgccg tcaaactatg acccggaaaa acgcacctac agtggtatct
      481 gggacgggag tctgaaaccg gcatacagca ataacccggc ctggtgcctg tgggacatgc
      541 tgacccaccc gcgctacggg atgggaaaac gcctgggggc cgcggatgtg gacaagtggg
      601 cgctgtatgc catcgggcag tactgcgacc agacggtccc ggatggtttc gggggcacag
      661 agccgcggat gacctttaat gcgtacctgt cacagcagcg taaggtgtgg gatgtcctgg
      721 gggatttctg ctcggcgatg cgctgtatgc cggtatggaa cggccagacg ctgacgttcg
      781 ttcaggaccg
//
LOCUS       DAZGVQ010000428          778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000428 DAZGVQ010000000
VERSION     DAZGVQ010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.432"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..122
                     /locus_tag="ACHLAG_005472"
     CDS             <1..122
                     /locus_tag="ACHLAG_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0658501.1"
                     /translation="YLISGGYSVIRNYFQRKIDERAAEKISQKSAQQNNQNMP"
     gene            138..605
                     /locus_tag="ACHLAG_005473"
     CDS             138..605
                     /locus_tag="ACHLAG_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0658502.1"
                     /translation="MLKINTLRDAMTDACPWCRANPEKFTVYVNNGGIETTGESPSYL
                     YRYTLGIFVMDFTQDLDELMVPLMRWLYRNQPDLLLNPEKNRDIKFSVAINDDDSTDI
                     LLEIPVWERVIIRRNDDGTVHAEHIGEPPVSRALEAWRVTLEDVTWGQNDGSG"
     gene            571..>778
                     /locus_tag="ACHLAG_005474"
     CDS             571..>778
                     /locus_tag="ACHLAG_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFP0658503.1"
                     /translation="MSHGVRMMAPDDLHRLDGLFQEILSGLSAAGINKTARAVGQAVR
                     AAQQKRIRSQQSPDGNRWPARKKRV"
BASE COUNT          204 a          207 c          205 g          162 t
ORIGIN      
        1 tgtatctgat cagcggcggt tacagtgtta tcagaaatta ttttcagcga aaaattgacg
       61 agcgcgccgc tgaaaaaatc agtcagaagt ctgcacaaca gaacaaccag aacatgccat
      121 aacacacagg agggggcatg ctgaaaatta acaccctgcg cgacgccatg actgacgcct
      181 gcccatggtg tcgcgccaac ccggagaaat tcactgtgta tgtgaataac ggaggcattg
      241 agacgaccgg cgaatccccc tcatatcttt accgttacac gcttgggatc tttgtcatgg
      301 acttcacgca ggatctcgat gaactgatgg tgccgctgat gcgctggctg taccgcaatc
      361 agcctgatct cctgcttaat ccggagaaaa acagggatat caaattttct gttgccatca
      421 atgacgacga cagcaccgat atcctgctgg aaatcccggt atgggagcgg gtgattatcc
      481 gccgtaatga tgatggcact gttcacgcag aacacatcgg agagccgcct gtcagccggg
      541 ccctggaagc atggcgcgtc accctcgaag atgtcacatg gggtcagaat gatggctccg
      601 gatgacctgc acagactgga cggtctcttt caggagatcc tcagtggtct gtccgctgcc
      661 ggtataaaca aaacagcacg tgctgtcggt caggctgtcc gcgccgcgca acaaaagcgt
      721 atacgttcgc aacaatcacc ggacggtaac cggtggccag cccgtaaaaa acgtgtat
//
LOCUS       DAZGVQ010000429          775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000429 DAZGVQ010000000
VERSION     DAZGVQ010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.433"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..656)
                     /locus_tag="ACHLAG_005475"
     CDS             complement(<1..656)
                     /locus_tag="ACHLAG_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP0658504.1"
                     /translation="MTVRISGVLKDGTGKPVPGCTIELKARRTTETVIVTTVAQGQPG
                     ETGSYSFDVEPGWYRVTLNTEGYAPSYVGDILVKADSEPGTLNKFLMEQDEAQYYPKA
                     LAELEAVAAEILKRAEASAASAEEAKKRAENARGPAGEKGDTGPQGVTGAQGPAGATG
                     AVGPKGEPGPKGERGETGPQGPKGDKGDPGGPPGPKGDTGPRGEAGPPGPQGPAGQTG
                     P"
BASE COUNT          105 a          286 c          192 g          192 t
ORIGIN      
        1 gggccggttt gccctgccgg accctgtggt ccgggtggtc cggcctctcc acgtgggccg
       61 gtgtcacctt tcggccccgg cggtccgccc gggtcacctt tatcgccctt cggtccctgt
      121 ggacctgttt ctccccgttc tccctttggc cccggctcac cttttggtcc gaccgccccc
      181 gttgccccgg ctggtccctg tgcccctgtg acaccctgtg gacctgtgtc ccccttctcg
      241 cccgccggtc cccgggcatt ctctgcccgt ttcttcgctt cctctgcact cgccgccgac
      301 gcttcagcac gcttcaggat ttccgctgcc accgcttcca gctctgcaag cgctttcggg
      361 taatactgcg cctcatcctg ttccatcaga aatttattca gcgttcccgg ctcagaatcc
      421 gccttcacca gaatgtcacc cacatacgac ggggcgtacc cttccgtgtt cagcgtcacc
      481 cggtaccacc ccggctccac atcaaaactg taactgccgg tttcccccgg ctgcccctgc
      541 gccaccgtgg tgactatcac tgtctctgtg gtgcgtcgcg ctttcagctc tatggtgcat
      601 cccggcaccg gcttcccggt gccgtccttc agcacgcccg atattctgac tgtcatagat
      661 ttctcccata aaaaaaaccg cagtaccggt tacccggtct gcgatcaggg gtataaattg
      721 tgactggctg gtgttaaagg gtgccgtcag tcaaaggcac ccgtggataa tgtac
//
LOCUS       DAZGVQ010000430          773 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000430 DAZGVQ010000000
VERSION     DAZGVQ010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.434"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>773
                     /locus_tag="ACHLAG_005476"
     CDS             <1..>773
                     /locus_tag="ACHLAG_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0658505.1"
                     /translation="GKAAQNIHFVPLMTDEHGVNVPTNEPSEDPDIIPAGYYGAASRT
                     ASNWTAADRKTHFSSWARRGLVSDRLAGAILQYAGRTLSFLTGQSAPQSGGTTPVSPG
                     TPDVEKPQDGGVAGTGHDEAVSSTRTVAEYDANSGNGALTEQQWGASGGKGTVTDDGG
                     RKALRLEKQPGKLTSWKMFRTIAVEEAKNLLSKGGEIAVRFKIPDGSELVNGQFVFGI
                     YWPVSQWASGATANSMLASFFLQTDTSNLNLMHQKGSSN"
BASE COUNT          189 a          179 c          246 g          159 t
ORIGIN      
        1 aaggaaaagc tgcgcagaat attcactttg tgccgttgat gacggatgag catggtgtga
       61 atgtgccgac aaacgagccg tcagaagatc cggacattat cccggcggga tactatggcg
      121 ctgcgtcacg cactgccagt aactggacgg cagccgatcg taaaacgcac ttcagttcat
      181 gggcgcgaag aggccttgtt tcagaccgcc tggcaggtgc cattttgcag tacgccgggc
      241 ggacattatc gttccttacc gggcagagcg caccgcagtc gggtggtaca acgcctgtca
      301 gtccggggac accggatgtt gagaaaccgc aggatggcgg tgttgctggt actggtcatg
      361 atgaggccgt gagcagtacc aggacggtgg ctgaatatga tgcgaacagt ggaaatggtg
      421 cattgacaga gcagcagtgg ggcgcttccg gcggcaaagg cactgtgact gatgacggtg
      481 gacggaaagc gctgcgactg gaaaaacagc ccggtaaact gacgtcctgg aaaatgttcc
      541 gtactattgc agtggaggag gcaaaaaatc ttctcagcaa ggggggggaa attgccgtac
      601 ggttcaaaat tccggatgga agcgagctgg tgaacggaca gtttgtcttt ggtatctact
      661 ggccggtgtc gcagtgggcg tcaggcgcga cagcaaacag tatgctggca tcattcttcc
      721 ttcagacgga tacatcaaat cttaatctga tgcaccagaa gggctcctcg aat
//
LOCUS       DAZGVQ010000431          766 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000431 DAZGVQ010000000
VERSION     DAZGVQ010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 766)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..766
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.435"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..470)
                     /locus_tag="ACHLAG_005477"
     CDS             complement(<1..470)
                     /locus_tag="ACHLAG_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658506.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIVNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPGGKCIITGDFQKELAYLQNVIIYNDSSLRMDFFGYNALIIQRSD
                     NTCELTINEPLKNQEISTGNINVNFPLKDIYNEIRRLNLVFSCGTGGIVDLSSL"
     gene            complement(492..>766)
                     /gene="yhaB"
                     /locus_tag="ACHLAG_005478"
     CDS             complement(492..>766)
                     /gene="yhaB"
                     /locus_tag="ACHLAG_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFP0658507.1"
                     /translation="IDISTGEHDDYIIQTLYVGTFAPFGEQCTCSAVNKKELECIKET
                     ISKYCAKFTRKEAILTPPAHFNKTSITSDCWQILFFSPDHFNNDFY"
BASE COUNT          247 a          124 c          134 g          261 t
ORIGIN      
        1 tccagagagg atagatcaac tatacctcca gtcccacaac taaaaactaa gttcaacctc
       61 ctgatttcat tgtaaatgtc tttcaatggg aaattaacat tgatattgcc tgtggatatt
      121 tcctggtttt ttaacggttc attaattgta agttcacagg tattgtctga tcgttgaata
      181 attagggcgt tataaccaaa aaaatccata cgaagagacg aatcattata aataattaca
      241 ttttgtaagt acgctaattc tttttgaaaa tcaccagtga taatgcattt tccacctggt
      301 tgtaacgcgt taagtaaagt gaccatttga atagaggtct gttttagttt ttctatacct
      361 ggtgctagtg cttcgaggtt attaactatg gggctttcag gaagatcatt catttttcga
      421 cggactaaat cactactaat atcattacca attgatgaaa cagggaacat tattttatcc
      481 tttataaata atcaataaaa atcattatta aaatgatcgg gtgaaaaaaa gagaatttgc
      541 caacaatctg aagtaatgct ggttttatta aagtgcgcag gaggtgttaa gatggcttct
      601 tttcgggtga attttgcaca atacttagaa atcgtctcct taatacattc cagctctttt
      661 ttattgacgg ctgagcaagt acattgttca ccaaaaggtg caaaagtgcc tacatataat
      721 gtttgtatga tataatcgtc atgttctcct gtagaaatat ctatga
//
LOCUS       DAZGVQ010000432          760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000432 DAZGVQ010000000
VERSION     DAZGVQ010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.436"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..207)
                     /gene="yhaB"
                     /locus_tag="ACHLAG_005479"
     CDS             complement(<1..207)
                     /gene="yhaB"
                     /locus_tag="ACHLAG_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFP0658508.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFY"
     gene            complement(463..681)
                     /gene="tdcR"
                     /locus_tag="ACHLAG_005480"
     CDS             complement(463..681)
                     /gene="tdcR"
                     /locus_tag="ACHLAG_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFP0658509.1"
                     /translation="MSKFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNT
                     GIDHLEQLINVNFFSSDRTSFCECNRFP"
BASE COUNT          233 a          138 c          137 g          252 t
ORIGIN      
        1 atagaaattc aatgcagtaa ttgcagataa tactctccgc agttcattac ttatactgta
       61 aattttatta acgtcattgg tggagacaat ttctgcaaac tttcttttta ccgtccaata
      121 atcaatattt ctattgtgat tgttaaccac tgcgtgcatt ggcgggattg aagggtgaaa
      181 aatatgtgca attgggaagc ctttcattga tgcctgcctt gtcgtcatga caaacaataa
      241 tagatgctta ttttcctcgc agggttcttt cgcactatca aaaaacgctc atttacccgg
      301 cgttacagta aacgaagcaa cattctgtgg cctacgacga tcaaaagctc tgtgaaaatt
      361 tcccttgttg tctttgtctg accttatcgc ggcgatcagc aattattcgt tttgtgatcc
      421 tcaacacaaa atgctattcg tcgcctatga ggggagaaga atttatggaa aacgattgca
      481 ttcacaaaat gacgtcctgt cagaagagaa aaagttgacg ttaatcaatt gttcgagatg
      541 atcaatacct gtattcgaaa ttttaaaata ttgataaaat aaatgtttgt tctccaacaa
      601 atagcgtgaa aaaatgtgac gtgccgcagt atttatcgct gaaaatggtt tatttataat
      661 aaaatttgag aacttgctca ttaaatttaa ctcaaatttt gcctggtaat tatccggtaa
      721 ttgcttgaaa tatggtctca gccccttttt tgtattaacc
//
LOCUS       DAZGVQ010000433          758 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000433 DAZGVQ010000000
VERSION     DAZGVQ010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.437"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..231
                     /locus_tag="ACHLAG_005481"
     CDS             <1..231
                     /locus_tag="ACHLAG_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658510.1"
                     /translation="AEFGYEIDIQPSPESQWISIVTVKTAVGYRHMNVLDDILTPLRI
                     YDASALECILNRYKPAWQTFLYLYENSHEETE"
     gene            231..>758
                     /locus_tag="ACHLAG_005482"
     CDS             231..>758
                     /locus_tag="ACHLAG_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0658511.1"
                     /translation="MYFVDNNSGVTDMPPLAPSQGTQVKWFTEGDGRKGISHIGQDWL
                     NIVQAELLAILTEGQVQPDKAKLNQLVIAIKAIIAANAYSRKNNLKEIADAGAEAQAA
                     ARRHLGLGGLSGKDSLAANDVGALEKSMNFGDVPDKTSARQNLDVYSKSEGDNRYLRK
                     DQNGADIADKGTFIDN"
BASE COUNT          223 a          162 c          208 g          165 t
ORIGIN      
        1 gcagagtttg gttatgaaat agatatccag ccatcaccgg aatcacaatg gatcagtatt
       61 gtgacagtga agacagcggt tggataccgt cacatgaatg tactggacga tattcttacg
      121 ccactgcgaa tttatgatgc aagtgcgctg gagtgcattt taaaccgtta taagccagcc
      181 tggcagacgt ttttatatct gtacgaaaac agtcatgagg agacggagtg atgtattttg
      241 tcgacaacaa ttcaggtgtg accgatatgc caccactggc cccctcacag ggcacgcagg
      301 ttaaatggtt tactgaaggt gacggacgca aagggatcag ccatataggt caggactggc
      361 tgaacatcgt gcaggcagag ctgcttgcca ttctgacaga aggacaggtt cagccggata
      421 aggcaaaact gaaccagctg gtgatagcca ttaaggcaat cattgcagca aacgcataca
      481 gccggaaaaa caaccttaag gagattgcgg atgcaggtgc agaagcacag gctgctgccc
      541 gccgtcatct tgggcttgga ggattatcag gaaaagacag cctggctgct aatgatgtgg
      601 gagcactgga gaaatccatg aattttggcg atgtaccgga taaaacctcg gcccgccaga
      661 atctggatgt ttacagcaag agcgaaggtg ataaccgtta tctgcgcaag gatcagaatg
      721 gcgcggatat cgcggacaaa ggaaccttta tcgataac
//
LOCUS       DAZGVQ010000434          758 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000434 DAZGVQ010000000
VERSION     DAZGVQ010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..758
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.438"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            44..634
                     /locus_tag="ACHLAG_005483"
     CDS             44..634
                     /locus_tag="ACHLAG_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0658512.1"
                     /translation="MSRIYAYCRVSTLEQTTKNQRREIESAGFNIKSQQIIEEQISGS
                     AATSERPGFNQLLDRLKRGDALIVTKLDRLGCNPMDISKTVEQLTKAGIKVHCLALGG
                     VDLTSPPGKMMMQVISAVAEFERDLLLERTHSGILRARGAGKRFGRPPVLNDEQIQRV
                     LEQIKSGINISDIAQEFKTSRQTILRAKAKNQTPDI"
BASE COUNT          250 a          152 c          160 g          196 t
ORIGIN      
        1 tgcattatga tatgtacacc aatttcgtat agaagatctc actatgtctc gaatttatgc
       61 ttactgtcgg gtatcaactc tggaacagac aaccaaaaat caacgtcggg aaatcgaaag
      121 tgcaggtttt aacatcaaat ctcagcaaat aattgaagaa caaattagcg gctcagcagc
      181 aaccagtgag cgtcctggtt ttaaccagtt gcttgatcga ctgaaaagag gtgatgcatt
      241 gattgtcaca aaactggatc gcctcggttg taatccaatg gatatcagta aaacggtgga
      301 acaactgacc aaagcaggta tcaaagtgca ttgcttagca ttggggggcg ttgacctgac
      361 cagtccacct ggaaaaatga tgatgcaagt aatttcagca gtcgctgaat ttgagcgaga
      421 ccttttactt gagcgtactc attcagggat attaagagca cggggcgcag gaaagcgatt
      481 tggtcgccca cctgtgttaa atgatgaaca aatacagagg gtactagagc aaattaagtc
      541 aggtataaat ataagtgaca ttgcccaaga attcaaaacc tcgcggcaaa ccattttaag
      601 agctaaagca aaaaatcaga cacctgatat ataaaaataa tctcggtgtg agattcctta
      661 cgttttccaa gccccccttc ctagccttaa atggaaagat acttctaatt atagaattta
      721 tatatcttac cctacggtag ggctggccat tcaatatc
//
LOCUS       DAZGVQ010000435          757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000435 DAZGVQ010000000
VERSION     DAZGVQ010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.439"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>757
                     /locus_tag="ACHLAG_005484"
     CDS             <1..>757
                     /locus_tag="ACHLAG_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFP0658513.1"
                     /translation="DFLNLKMMNQPYRADILNAITKVVDSGWYIQGEEVRNFEHEFAS
                     FIGSKYCVGVANGLDALSITLKAWILLGKIKEGDEVIVPANTFIATVLAITQNHLKPI
                     FVEPDEETFNINVDMIRNVITEKTKVIIPVHLYGQVAEMNKIRELADLHDLLILEDSA
                     QAHGAMYNNLKAGNLGDAGAFSFYPGKNLGALGDAGAVVTDNQELAETIRAIGNYGAH
                     VKYKHDVQGVNSRLDEIQAAILRVKLKYINNEIE"
BASE COUNT          263 a          110 c          167 g          217 t
ORIGIN      
        1 agacttccta aatctaaaaa tgatgaatca accttatcga gcggatatac taaatgcaat
       61 aactaaagtt gttgattcgg ggtggtatat tcaaggtgaa gaagtcagaa atttcgaaca
      121 tgagtttgca tcatttattg gttcgaagta ttgtgtcggt gttgccaatg gcttagatgc
      181 attgagcatt accttaaaag catggatatt attaggcaaa attaaagaag gggatgaggt
      241 aatagttcca gcaaatacat ttattgcaac tgtattagct atcacccaaa atcatcttaa
      301 accaatattt gtagaaccag atgaagaaac attcaacatt aatgttgata tgattagaaa
      361 tgtcatcacg gagaagacta aagttattat acctgttcat ctttatgggc aggtggcaga
      421 aatgaataaa attcgtgaat tagctgacct tcatgatctg ttgattttgg aggactctgc
      481 gcaagcacat ggagccatgt ataataacct taaggcagga aaccttggtg atgcaggcgc
      541 ttttagtttc tacccaggta aaaacttggg ggcattaggg gatgctggtg ctgtagtgac
      601 tgacaatcaa gagctggcag aaactataag agcaattggt aattatggtg cacatgtaaa
      661 atataaacat gatgtacaag gggttaatag ccgtctagat gagattcaag cagcgattct
      721 acgggttaaa ttaaaatata taaacaatga aatagaa
//
LOCUS       DAZGVQ010000436          757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000436 DAZGVQ010000000
VERSION     DAZGVQ010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.440"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            295..474
                     /locus_tag="ACHLAG_005485"
     CDS             295..474
                     /locus_tag="ACHLAG_005485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0658514.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            515..>757
                     /locus_tag="ACHLAG_005486"
     CDS             515..>757
                     /locus_tag="ACHLAG_005486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP0658515.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQQLRQNLEARLDAEVDA
                     ILDELLGAPTVPEPEGIADDSAVSDGVGSQPDGSEEP"
BASE COUNT          208 a          170 c          211 g          168 t
ORIGIN      
        1 cagagcagac ggcgtttgac cttgtgatgt ggacaaatga tggctttaca gcagatacgc
       61 tgacgctgac agatatcacg ggggcaaaag cgacgtatcc tgtactgctt gatacggtca
      121 cagtcaaagt taacgaaagc cgggcatcat cataaccggc aaaaaaagcc gccagcggca
      181 ggaacggaag ctggcggagg taatcccaat ggagaatgta aagaaaagat gctttcgtat
      241 atcggttttt taaatgaaaa cagttctcat tgtcaaccat aacggtaaga aactatgaca
      301 tttattcatc aggtgatgct gtacttctgt acggcggtct gtgtgctgta tcttctttcg
      361 ggtgggtaca gggcagtgcg cgatttctgg cgcaggcaga ttgataaaag ggccgctgag
      421 aaaatcagcg ccagtcagtc agccggaagc aaacccgaag agccgctcat ttagctgcaa
      481 ctttcttaat cacacctttc gacgagaaaa tcccatgtca gaaattacat ccctggtcac
      541 tgcagaggca gtgaaggaag tcctgcgctc tgaagaagtc agaagcgcac tgaaacagca
      601 acttcggcag aaccttgagg cgcgtcttga tgcagaagtg gatgccattc tggatgaact
      661 gctgggcgca ccgactgttc cggagccgga aggcattgcg gatgacagtg ctgtttcaga
      721 tggcgtcggg tctcagcctg acggtagtga agagcct
//
LOCUS       DAZGVQ010000437          754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000437 DAZGVQ010000000
VERSION     DAZGVQ010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.441"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            24..224
                     /locus_tag="ACHLAG_005487"
     CDS             24..224
                     /locus_tag="ACHLAG_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP0658516.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMKNNEK"
     gene            complement(415..>754)
                     /locus_tag="ACHLAG_005488"
     CDS             complement(415..>754)
                     /locus_tag="ACHLAG_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP0658517.1"
                     /translation="FDKKQIYYRMDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAE
                     LRKFSESYEAERNAVMLEYMKEKDVASMSGRLLNRWGRVKKPQLLARLDRLEQQGQIA
                     LPGFDKGISA"
BASE COUNT          199 a          212 c          170 g          173 t
ORIGIN      
        1 ggcaatcagt aaaggaaata aatatgtcat cgaaaaaccg gacccgcaga accacaaccc
       61 gcaatatccg tttccccaat cacatgattg aacagatcaa catcgccctt gagcataaag
      121 ggtccggtaa cttttcagcg tgggttattg aagcctgcag gagaaggctg gcaacagatg
      181 caacgcatct gcgcccggcc agcatgaaaa ataacgagaa atgaacgttc ggtttcttcc
      241 accatcgcac cggacaggcg actatgaggg gacaacgccg cgctccgtta acgcggtaaa
      301 ccccggtgtg catcgttttt gattatcccc gcacactcgc gcagaggagt ctccctgtcg
      361 ggctgcggtc tctgttaatg caggaatacg gcgacgatac ggcgcatggc tatgtcaggc
      421 tgaaatgcct ttatcaaatc cgggtaacgc aatctgcccc tgctgctcca gcctgtccag
      481 ccttgccagc aactgaggct tcttcaccct gccccagcga ttgagcaaac gacctgacat
      541 actggcaaca tctttctctt tcatgtactc cagcattaca gcgttgcgtt cagcttcata
      601 actttcactg aacttacgga gttcagcgga catccagtta aaggcattaa tataggcttc
      661 tttaacggca tcagcttttg ccccgttaaa tcccataacc agcataacga agccgctaaa
      721 gtccatgcgg tagtaaatct gcttcttgtc gaaa
//
LOCUS       DAZGVQ010000438          735 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000438 DAZGVQ010000000
VERSION     DAZGVQ010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.442"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..570)
                     /gene="fimE"
                     /locus_tag="ACHLAG_005489"
     CDS             complement(<1..570)
                     /gene="fimE"
                     /locus_tag="ACHLAG_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFP0658518.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLIN"
BASE COUNT          228 a          178 c          148 g          181 t
ORIGIN      
        1 gtttatgaga ttatttcttt cccatagtcc ggcaaaacga gcagcattac tggcggtata
       61 acgtacagta tggcgaatat ttcgatgccc gagataatcc tgaattaaac gggtatcggc
      121 accacgctcc gccagttcat aaccacaagc atgccttaac atatgaggat gagtctgcgt
      181 tacggttcca gcttcaatac cggcatcgcg aataatgcga taggcctgct ggcgagaaag
      241 ccgactcccg cggcgagaaa taaatatagc gtcggtccgg tcagcgcctt tccagttagc
      301 acgttcctgg gtccagcgtt ccacggcttc acgctcatca aaacgtaacg ggtgaacggt
      361 agaaaatccg ttcttcagtc ggcgaatatt tattcgacct tcattaaggt caagatcctg
      421 ataatgcaga tcaagcagtt cactaatacg catcccatgc cgatatgcca acagaataag
      481 acaataatct ctggctcccg ttgccccgta acaaaccgcc tgcatcatgg cctgaacttc
      541 tttaccggta agataacgac gtttactcac aatagtagta ctcctaactg agatatattt
      601 gaatgaatac ctataggaaa cctcaatcgg tcaaaattag cccaatagat aaaaagataa
      661 ccacacacga agtgatgtgg ctatcagtca attacattaa atatcataca aaaataaaat
      721 atcactgatg tgata
//
LOCUS       DAZGVQ010000439          734 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000439 DAZGVQ010000000
VERSION     DAZGVQ010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.443"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>734
                     /locus_tag="ACHLAG_005490"
     CDS             <1..>734
                     /locus_tag="ACHLAG_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP0658519.1"
                     /translation="DSFGGMIPMFRGLAGAITLPMVGVTSLAVATGALAYAWYQGDST
                     LSEFNKTLVLSGNQAGLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFD
                     AINQSVARFASASGVEVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQR
                     SGDEAGALQAANDIATKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPE
                     SSADMLASAQKAFDEADKKWQWYQSR"
BASE COUNT          160 a          183 c          247 g          144 t
ORIGIN      
        1 aggactcctt cggcgggatg atccccatgt tcagggggct tgccggtgcg atcaccctgc
       61 cgatggtcgg ggtcacctcg ctggcggtgg cgacaggtgc gctggcgtac gcctggtacc
      121 agggggattc cacgctttca gaatttaata aaacgctggt cctttccggc aatcaggccg
      181 gactgactgc cgatcgtatg ctgacgctct caagagccgg gcaggcagca gggctgacgt
      241 ttaaccaggc gagagagtca ctggcagccc tggtgaatgc cggtgtgcgt ggtggtgaac
      301 agtttgatgc cattaaccag agtgtcgcgc gttttgcgtc tgcatccggt gtggaggtgg
      361 ataaagtcgc tgaagccttc gggaagctga ccactgaccc gacgtcggga ctgatggcga
      421 tggcgcgcca gttccgtaac gtgacggcag agcagattgc gtatgttgca caactgcagc
      481 gttccggaga cgaggccggg gccttacagg cggcgaacga tatcgccacg aaaggctttg
      541 atgagcagac ccgtcgcctg aaagaaaaca tgggaacact ggagacctgg gcggataaaa
      601 cagggaaggc attcaaatcg atgtgggatg ccattctgga tatcggtcgt ccggaatcct
      661 cagcggatat gctcgccagt gcgcagaagg catttgatga ggcggataaa aaatggcagt
      721 ggtaccagag ccgg
//
LOCUS       DAZGVQ010000440          733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000440 DAZGVQ010000000
VERSION     DAZGVQ010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.444"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            43..>733
                     /locus_tag="ACHLAG_005491"
     CDS             43..>733
                     /locus_tag="ACHLAG_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP0658520.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQK"
BASE COUNT          243 a          127 c          163 g          200 t
ORIGIN      
        1 atttaccagt aatgtaaata aattttcgag gagatcattc cagtgggacg taaatgggcc
       61 aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta tgcaaaattc
      121 ggtgtagaaa tctatgctgc tgctaaacaa ggtgaacccg atccagaatt aaacacatct
      181 ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt tattgataaa
      241 gcaattgata aagccaaagg cggcggagat gaaacgttcg tgcagggacg ttatgaaggc
      301 tttggtccta atggctcaat gattatcgcc gagacattga cttcaaatgt taaccgtacg
      361 attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc ggcaggttct
      421 gtcagctata tgtttgacaa tacgggtgtg attgtattta aaggatcaga ccctgaccat
      481 atttttgaaa ttttacttga agctgaagtt gatgttcgtg atgtgactga agaagaaggt
      541 aacattgtta tttataccga acctactgac cttcataaag gaatcgcggc tctaaaagca
      601 gctggaatca ctgagttctc aacaacagaa ttagaaatga ttgctcaatc tgaagttgag
      661 ctttccccag aagatttaga aatctttgaa gggcttgttg atgcccttga agatgacgac
      721 gatgtacaaa aag
//
LOCUS       DAZGVQ010000441          729 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000441 DAZGVQ010000000
VERSION     DAZGVQ010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.445"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>729)
                     /locus_tag="ACHLAG_005492"
     CDS             complement(<1..>729)
                     /locus_tag="ACHLAG_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFP0658521.1"
                     /translation="GNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPEI
                     VWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAIN
                     FSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEG
                     IPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMD
                     FAELLLEDYKASLKYLSDHPKLQGI"
BASE COUNT          169 a          205 c          205 g          150 t
ORIGIN      
        1 aataccctgc agtttcgggt gatcgctgag atatttcagg gaggctttgt agtcttccag
       61 caacagttca gcaaagtcca tttcgaagcc gcgacgacac ataatgcgca tcaccacgat
      121 gtcggtggct tcaccgccga gagtgaaggc cggaacctgc cagccgcgca gacgcagacg
      181 ttcagagagg tcgtacaggg tgtatcccgg atcttcacca tctttcagtt tgaagcaaac
      241 cgccgggatg ccttcgtccg ggcgacccgt acagatgaac tcatacggcc ccagtttggc
      301 gatttcatcc gccagataag cggcaacctg gtaagaggcg ttctgtactt tggtatagcc
      361 ttcacgaccg aggcgcagga attcatagta ctgtgcaatt acctgacccg ccgggcggga
      421 gaagttgatg gcaaaagtac caatttgacc acccaggtag tcaacgttga acaccagttc
      481 ctgcggcagc gcttcttcgt cacgccagat aacccagccg cagcccagcg gagccagacc
      541 gaatttatgg cctgaagcac tgatcgattt cacacgcggc aggcggaagt cccagacgat
      601 ttccggggcg acgaacggtg ccaggaagcc accgctggca gcgtcgatgt gcatgtcgat
      661 gtcgataccg gtgtcggcct ggaatttatc cagcgcatcg tgcagcggtt gtgggaactc
      721 atagttacc
//
LOCUS       DAZGVQ010000442          728 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000442 DAZGVQ010000000
VERSION     DAZGVQ010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.446"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(132..704)
                     /gene="lpfA-O113"
                     /locus_tag="ACHLAG_005493"
     CDS             complement(132..704)
                     /gene="lpfA-O113"
                     /locus_tag="ACHLAG_005493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFP0658522.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          209 a          176 c          150 g          193 t
ORIGIN      
        1 aaaaacgctg agtataatcg cggtaaataa cttacatgaa actgttgttt taaagagcct
       61 gattaaacaa ctttttctgg acataacgac tccctggcca aagccaactg aataaaaagg
      121 cccttgataa attatttctt atattcgaca gtaaattgtg aatcagcgtt cgccgtacca
      181 gccgttacgg tcgcattggt tgccacatag cgtgcaataa acttcatttc agccttgcct
      241 tcagcatcga tagcggtata agcagagtcg ttataaagtt taacctgcga attatcggac
      301 tggttaaaaa tacgaatacc aacgccggtt gccgcaatcg cttgtcctgt accggtaaaa
      361 tcaccagggt tgcctgctgt aagcggcgta cccactttca ggtcgccgtc gccgccaggc
      421 gcttctgtac catcaaaacg aacggctgct tgagtgtacg ttgccgggca attttcaagc
      481 ttaatactga acttctgtgg cgacgccaga ctacctacac ctgaaaatgc atttttactg
      541 actttaccca gtgggacaat ttgatcggct gtatcggagg taacttcaca agtagtgtcg
      601 acaatttcac cgacgaagtg aacaacacca tcttctgcca gtgcatgccc ggccagcatt
      661 agagatgcca gagtgaatgc accgcctata atattacgct tcataaaaac tccattgata
      721 taatatat
//
LOCUS       DAZGVQ010000443          715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000443 DAZGVQ010000000
VERSION     DAZGVQ010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.447"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..256
                     /gene="yhhH"
                     /locus_tag="ACHLAG_005494"
     CDS             <1..256
                     /gene="yhhH"
                     /locus_tag="ACHLAG_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HFP0658523.1"
                     /translation="ESEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEIT
                     ELTTSWVVEGTIHSDQIAGGVFIIEIGKNDGRILNFGHGK"
     gene            361..>715
                     /locus_tag="ACHLAG_005495"
     CDS             361..>715
                     /locus_tag="ACHLAG_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HFP0658524.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCEDNVFNILNNMALND
                     KSACVRATAIESTAQR"
BASE COUNT          260 a           97 c          165 g          193 t
ORIGIN      
        1 tgaaagtgaa ggtaaatata ccgttagaaa tttggttaaa aataaagcta tagcattgga
       61 attagctgag atatatgtta agaatcgtta tggacaggat gccgcagaag aagaaaaacc
      121 atacgaaatc actgagttaa caacaagttg ggttgttgaa ggtaccattc actcagacca
      181 aattgctggt ggggttttta ttatagaaat aggcaaaaat gatgggagaa ttctgaattt
      241 tggccacgga aaataagtat gtaaggataa tcaaacatcg tgtgcgctga tggcagagta
      301 ggtggaggac tccagacagt caaacgatag aaaaagatag cctttatgga ggttcctgca
      361 atgtcaaata cataccagaa aagaaaggca agtaaagagt atggtttata taatcaatgt
      421 aagaaactaa atgatgatga attatttcgc ttacttgatg atcacaattc cttgaaaagg
      481 atttcatctg ccagagtatt acagttaaga ggtgggcaag acgctgttag attggcaatt
      541 gagttctgct ctgataaaaa ttatatccgt agagatatcg gagcatttat actcgggcaa
      601 ataaaaattt gcaaaaaatg cgaagataat gtttttaata ttttgaacaa tatggcattg
      661 aatgataaga gcgcttgcgt tcgagctacg gcaatcgagt caacggctca gcgat
//
LOCUS       DAZGVQ010000444          709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000444 DAZGVQ010000000
VERSION     DAZGVQ010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.448"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..222
                     /locus_tag="ACHLAG_005496"
                     /pseudo
     CDS             1..222
                     /locus_tag="ACHLAG_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            261..>709
                     /locus_tag="ACHLAG_005497"
     CDS             261..>709
                     /locus_tag="ACHLAG_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFP0658525.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNVINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFNATNYDRLVKLSLNSNTLESINYPQG"
BASE COUNT          256 a          151 c          114 g          188 t
ORIGIN      
        1 gcacacgctg ccgaagtcta taacaaggat ggtaataaac tcgaccttta tggcaaggtt
       61 accgctctac gttattttac tgatgataag cgtgacgatg gtgataaaac ttatgcccgt
      121 ctcggcttta aaggagaaac gcaaatcaat gatcaaatga ttggttttgg tcactgggaa
      181 tatgatttta aaggctataa cgatgaagcc aacggctcgc gcggcaagaa tctctgattg
      241 agttaagtgc ccatcacaat atgatcactg accttatatt acacaatcat cccaggatga
      301 aaacaatcac tttaaacgac aaccatattg cacatttaaa cgccaaaaac actacaaaac
      361 tggaatattt aaacttaagc aataacaatt tactgccaac caatgacatt gatcaactaa
      421 tatcatcaaa acatctttgg catgtattag ttaacgtcat caacaatgat ccacttgcac
      481 aaatgcagta ctggactgca gtaagaaata taattgatga cactaatgaa gtgaccattg
      541 atttatcagg acttaattta accactcaac caccagggct gcaaaacttc acctctatca
      601 atcttgataa taaccaactc acacatttta atgcaaccaa ctacgataga ctcgtaaagc
      661 taagtctgaa tagtaatact cttgagtcaa taaattatcc tcaaggcag
//
LOCUS       DAZGVQ010000445          708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000445 DAZGVQ010000000
VERSION     DAZGVQ010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.449"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>708
                     /locus_tag="ACHLAG_005498"
     CDS             <1..>708
                     /locus_tag="ACHLAG_005498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFP0658526.1"
                     /translation="LTAVKDTQGHETRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAI
                     CTTQGGLTRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLT
                     GKLIRSEDEGLVTHWHYDEADRLTHRTVNGETAEQWQYDERGWLTDISHISKGHRVTV
                     HYGYDEKGRLTGERQTVHHPQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYG
                     SGYLAGMKLGDTPLVEYT"
BASE COUNT          177 a          210 c          220 g          101 t
ORIGIN      
        1 gttaactgcg gtgaaagaca cgcagggcca tgaaacgcgg tatgaataca acgccgccgg
       61 tgacctgacc gccgtcattg ccccggacgg cagcagaaac gggacacagt acgatgcgtg
      121 gggaaaagcc atctgtacca cgcagggcgg tctgacgcgc agtatggaat acgatgctgc
      181 cggacgggtc atccgcctga ccagtgaaaa cggcagccac accaccttcc gttacgatgt
      241 actcgaccgg ctgatacagg aaaccggctt tgacggccgc acacagcgtt atcaccacga
      301 cctgaccggc aaacttatcc gcagcgagga tgaggggctg gtcacccact ggcactatga
      361 cgaagcagac cgcctcacgc accgcaccgt gaatggcgaa accgcagagc agtggcagta
      421 tgacgaacgc ggctggctga cagacatcag ccatatcagc aaagggcacc gggtgacggt
      481 gcattacggg tatgatgaga aaggccggct gaccggtgag cgtcagacgg tgcatcaccc
      541 gcagacggaa gcactgctct ggcagcatga gaccagacat gcgtacaacg cgcaggggct
      601 ggcgaaccgc tgtataccgg acagcctgcc cgccgtggaa tggctgacct acggcagcgg
      661 ttacctggca ggcatgaaac tcggcgacac accgctggtg gagtacac
//
LOCUS       DAZGVQ010000446          706 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000446 DAZGVQ010000000
VERSION     DAZGVQ010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.450"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            130..706
                     /locus_tag="ACHLAG_005499"
                     /pseudo
     CDS             130..706
                     /locus_tag="ACHLAG_005499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          203 a          159 c          130 g          214 t
ORIGIN      
        1 cactgtaaaa gatacttttt gtatagatct aacaaccgta ataaaacaaa cgctcataac
       61 tcattataaa taatgataaa ataacgcctt ttattgataa tttacatatg aaaaaaattt
      121 ccttttgtta tattatttct gttttcactc cctctgatta ttttttacca accctgggtc
      181 actgcattat cgccaatgcc ccgacacgta agtcctgagc aattagaaaa aacagttcgt
      241 tatcttacac aaactgtgca tccacgtaat gccggcaata ttgataatct gaataggtcc
      301 gtagagtata taaaagaggt ctttgtcagt agcggtgcca gagttaccgc gcagggcgtc
      361 cccgattggc tattacgact ctgcgcctgg aagccagaat tacccttacc cggcaatgtc
      421 ctggctttat cccgatcggg gagactttat tgccgtagtc ggcagaatgc aggatatcaa
      481 cgccgttcgt caggtaaaat cggcattgtt gtcatcccgg gatttatctg tttattctat
      541 gaatgcccca gggtttattc ccggtattga tttctctgat caccttaata attggcaaca
      601 cgatattccc gccgtaatga ttactgacac cgctttttat cgtaataaac aataccactt
      661 gcccggtgat accgcagaca gattgaatta tcagaaaatg gctcag
//
LOCUS       DAZGVQ010000447          704 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000447 DAZGVQ010000000
VERSION     DAZGVQ010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..704
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.451"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            231..>704
                     /locus_tag="ACHLAG_005500"
     CDS             231..>704
                     /locus_tag="ACHLAG_005500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658527.1"
                     /translation="MSHFRDLYLANKKSRDARREVTGILRNQAEKLVAFYEQWLELPS
                     ETWEDKDGEHHRYVDTGFPCRNDRDFRPFPVNMIATDTDNIFRMAVRTWVDSHDEGES
                     VNIVLAMELHSVGKDGVSLRVRVEDDTSIRVVINGDGDREWLPIVNSMKMHIISKL"
BASE COUNT          219 a          132 c          206 g          147 t
ORIGIN      
        1 aaacatggtc agggaaacag gccgtcagaa tgcgtctgat tgcgtctggc gaggtcggag
       61 atcgggaggg aaacggatgt gacgaacggg ataaaaaaga gagacaaaaa tcactgaaat
      121 cataaagtta atagaattta aagtatcagt cattcacggg tgaaaacttg cgcacaatat
      181 gaggtgagcg cagaatacag gctggcataa acagaccaaa ggagagcgac atgagccatt
      241 ttcgcgattt gtacctggca aacaaaaaga gccgggacgc gaggcgggag gttacaggga
      301 ttctgcgcaa tcaggctgaa aagctggttg ctttttatga gcagtggctg gaattgcctt
      361 cagagacatg ggaagacaaa gacggcgagc atcatcgtta cgttgatacg gggtttccgt
      421 gcagaaacga tcgggacttc aggccatttc cggtaaatat gattgcaacc gacacagata
      481 acatattccg gatggctgta agaacatggg ttgactcaca cgatgaagga gagtcggtaa
      541 acatagtcct ggcaatggag ttacattccg tcggaaaaga cggtgtatct cttcgggttc
      601 gcgtggaaga tgatacctcc attcgcgtgg tcatcaacgg agacggtgac agggaatggc
      661 tgccgatagt aaacagtatg aagatgcata ttatcagcaa gctg
//
LOCUS       DAZGVQ010000448          691 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000448 DAZGVQ010000000
VERSION     DAZGVQ010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.452"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>691
                     /locus_tag="ACHLAG_005501"
     CDS             <1..>691
                     /locus_tag="ACHLAG_005501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP0658528.1"
                     /translation="HARRCAPAESCGFVISTPEGERYIPCVNISAEPEAYFRIAPEDW
                     LRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHKFRCVPHLTGR
                     RFAHGVTDCYTLFRDAYHLAGTEMPDFHREDEWWRNGQNLYLDNMEATGFYRVPLSSA
                     QAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSVWRHRHW
                     HASAFTGIYNDL"
BASE COUNT          131 a          190 c          226 g          144 t
ORIGIN      
        1 catgcccggc ggtgtgcgcc tgcggagtcg tgcggcttcg tgataagcac gccggagggg
       61 gagcggtata tcccttgtgt gaatatttcc gcagagccgg aggcgtattt tcgtatcgca
      121 ccggaagact ggctgcgggc agagatgcag ggggagattg tggcactggt ccacagtcat
      181 cccggtgggc tgccctggct gagcgaggct gaccggcggc tgcagataaa aagtgccctg
      241 ccctggtggc tggtctgccg gggtgacatt cacaaattcc gctgcgttcc gcacctgacc
      301 ggacggcgct ttgcgcacgg ggtgacggac tgttacacgc tgttccggga tgcataccat
      361 ctggcgggga ctgaaatgcc ggattttcac cgcgaggatg agtggtggcg caacggccag
      421 aacctgtacc tggacaatat ggaggccacg ggcttttacc gggtgcccct gtcctctgca
      481 caggcgggcg atattctgct gtgctgcttt ggcgcatcgg tggccaacca tgccgccatt
      541 tactgcggca acggtgaact gcttcaccat ctgcctgaac aactgagtaa acgggagagg
      601 tattccgaaa aatggcaacg acgaacgcat tcagtctggc gtcaccgcca ctggcacgca
      661 tctgccttca cggggattta caacgatttg g
//
LOCUS       DAZGVQ010000449          684 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000449 DAZGVQ010000000
VERSION     DAZGVQ010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.453"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>684
                     /locus_tag="ACHLAG_005502"
     CDS             <1..>684
                     /locus_tag="ACHLAG_005502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP0658529.1"
                     /translation="GFRLFCEGGVRPPLEKMMPLLDKLREQYGVRPVCSELHIAPSTY
                     YHCQQQRHHPDKRSARAQHDDWLKKEIQRVYDENHQVYGVRKVWRQLLREGIRVARCT
                     VARLMAVMGLAGVLRGKKVRTTVSRKAVAACDRVNRQFVAERPDQLWVADFTWVSTWQ
                     GFVYVAFIIDVFAGYIVGWQVSSSMETTFVLDALEQALWARRPSGTIHHSDKGSQYVS
                     LAYTQRLKEA"
     misc_feature    8..124
                     /locus_tag="ACHLAG_005502"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
BASE COUNT          159 a          167 c          205 g          153 t
ORIGIN      
        1 aggcttccgc ttattttgcg aaggcggagt tcgaccgcct ctggaaaaaa tgatgccact
       61 gctggataag ctgcgtgagc agtacggggt cagaccggta tgcagcgaac tgcatattgc
      121 cccgtcaacg tattaccatt gtcagcaaca gcgacatcat ccggataaac gcagtgcccg
      181 tgcgcagcac gatgactggc tgaagaaaga gatacagcgc gtatacgatg aaaatcacca
      241 ggtatacggt gtgcgtaaag tctggcgtca gttgttacgg gaaggtatca gagtggccag
      301 atgcactgtg gcacgtctca tggcagttat gggacttgcc ggtgttctcc ggggtaaaaa
      361 ggtccgcact accgtcagcc ggaaagccgt tgccgcatgc gaccgcgtaa accgtcagtt
      421 cgtggcagaa cgtccagacc agttgtgggt ggctgatttt acctgggtaa gcacatggca
      481 gggcttcgtt tatgtggcgt tcatcattga tgtgttcgcc ggatacatcg tgggatggca
      541 ggtctcatca tccatggaaa caacattcgt gctggatgca ctggagcagg cgttgtgggc
      601 ccgtcggccg tccggcacaa tccatcacag tgataaaggt tctcagtatg tatcgctggc
      661 ctacacgcag cggcttaagg aagc
//
LOCUS       DAZGVQ010000450          672 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000450 DAZGVQ010000000
VERSION     DAZGVQ010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.454"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     repeat_region   59..514
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=59..87
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT          173 a          171 c          172 g          156 t
ORIGIN      
        1 agttggtaga ttgtgactgg cttaaaaaat cattaattaa taataggtta tgtttacaga
       61 gttccccgcg ccagcgggga taaaccgccg cttaaccgga cctcatcaca aaacacgcgg
      121 agttccccgc gccagcgggg ataaaccggc tatcgggtgt ggataccgct ttcgaggcgt
      181 gagttccccg cgccagcggg gataaaccgg actcgggtgg caagtattcc ccgaatgctg
      241 ggagttcccc gcgccagcgg ggataaaccg cctgttgcgc cttataatgt gtataccgcg
      301 aagagttccc cgcgccagcg gggataaacc gacgctgatt tgatgggggc tggtgcagga
      361 gatgagttcc ccgcgccagc ggggataaac cgtttcagca gttcagcgta acaccgacgg
      421 tcacgagttc cccgcgccag cggggataaa ccgcccggaa tgcattctga aggtttgctg
      481 tatatgagtt ccccgcgcca gcggggataa accaccatat aacccgttat ctctttctca
      541 agtttttata ttagcagtac ttgtaataag caacatatcc acgtaacacc tcatgttcaa
      601 aatagttctc catgccagag aggttcacaa ttatcgatac aaaaaattaa atttaatcaa
      661 agtgttattt gt
//
LOCUS       DAZGVQ010000451          660 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000451 DAZGVQ010000000
VERSION     DAZGVQ010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.455"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..649
                     /locus_tag="ACHLAG_005503"
     CDS             <1..649
                     /locus_tag="ACHLAG_005503"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFP0658530.1"
                     /translation="DKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRF
                     CSIASNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGN
                     DVWIGNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDEL
                     LRIKWWNYNYSDLPDNNKCDDINYFVEEMNRLISDGNIQERDCKKFNLSEVFRGL"
BASE COUNT          214 a           92 c          137 g          217 t
ORIGIN      
        1 tgacaagata acaattgaag ataatgttat tgctgaagaa tattcaacaa tgcctgtgaa
       61 aaatttctcc agtgtcggcg ctttttcatt tccgacctgc catttttcag ggaatatacg
      121 tataggtcga ttttgtagta ttgcatcaaa tgtaaaaata atgggtggaa atcatccttt
      181 gaatcgtttt actactcaca tgatgactta taacggtgaa tttgataagt ttgctatgag
      241 tgaatttgaa cgtagctgga cattgaaacc attcattacc aaacctgaaa atccgattat
      301 aggaaatgat gtctggatag gaaatgatgt tgtattgaaa ggtggcatcg caataggtga
      361 tggtgctgtt attgctgcca attccgttgt taccaaagat gtgcccccat atgccatagt
      421 tgcaggagtt cctgcaaaaa taatcagatt taggtttgat tcaaatgtta ttgatgaact
      481 gttgagaata aaatggtgga attataacta ctcagatctt ccggataata acaagtgtga
      541 tgatataaat tattttgttg aagagatgaa tcgattgata tcagatggaa atattcaaga
      601 gagggactgt aaaaaattca atctttctga ggtattcaga ggcctataat tatctgtttt
//
LOCUS       DAZGVQ010000452          657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000452 DAZGVQ010000000
VERSION     DAZGVQ010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.456"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            137..499
                     /locus_tag="ACHLAG_005504"
     CDS             137..499
                     /locus_tag="ACHLAG_005504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFP0658531.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT          193 a          151 c          156 g          157 t
ORIGIN      
        1 ttaatcaata aattgcgtag tttttaagca tgttaatgat tacgttacag aactattgca
       61 atgtcatcag ttgtgaacaa agcacctggt cgcgcatact aggggctata aatttatctt
      121 tatcagaagc catcacatga aagaagtcga aaaaaacgaa atcaaacgtc tcagcgatcg
      181 tctggacgcc atccgccacc agcaggccga tctgtcgctg gttgaagccg cagacaaata
      241 tgccgagctg gaaaaagaga aagccacgct ggaagcagaa attgctcgcc tgcgtgaagt
      301 tcatagccaa aaactgagta aagaagcaca gaagctgatg aagatgccgt ttcagcgcgc
      361 aattaccaaa aaagagcagg ctgatatggg caagctgaag aaaagtgttc gcggactggt
      421 ggttgtgcac ccaatgaccg cgctgggccg tgaaatgggc ctgcaggaga tgactggatt
      481 ctctaagact gcgttttaag cgcctcagac gactggttcg atcatatcaa cccgcatctg
      541 gcttgcctta gctggtcaat gcggggactc tcctgtctac tcgcctaatt accaaacaat
      601 tcactccctt tacgtcgatt taccgctggt tgatattctg aaaatgcatt tttaccg
//
LOCUS       DAZGVQ010000453          653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000453 DAZGVQ010000000
VERSION     DAZGVQ010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.457"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..488
                     /locus_tag="ACHLAG_005505"
     CDS             <1..488
                     /locus_tag="ACHLAG_005505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP0658532.1"
                     /translation="TETIEIFNNSDEWANQLKHLLSKGENLALLHGLTPDILDRIYAY
                     AFDYHEKGNVTDAEIYYKFLCIYAFENHEYLEGFASVCQSKKKYQQAYDLYKLSYNYF
                     PYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSEDN
                     G"
BASE COUNT          241 a          100 c          106 g          206 t
ORIGIN      
        1 gcacagaaac aattgaaata ttcaataata gtgatgaatg ggcaaatcaa ctaaaacacc
       61 tattatcgaa aggagaaaat ctggcattac tacatggttt aactcctgat atccttgata
      121 gaatatatgc atatgcattc gactaccatg aaaagggtaa tgtaacggac gcagaaattt
      181 attataaatt tttgtgcatt tatgcgttcg aaaatcatga gtatctagaa ggttttgcat
      241 cagtatgtca gtcaaaaaag aaatatcaac aagcatatga cctttacaaa ctaagttaca
      301 attacttccc gtatgatgac tattcagtta tttatcgtat gggtcaatgt cagattgggg
      361 ctaaaaatat cgataacgca atgcaatgtt tctatcacat tattaacaat tgtgaggatg
      421 atagtgttaa gagtaaagcg caggcatata ttgaactctt aaacgataat tcagaagata
      481 atggctaagc attacaatca tttggttaaa tttgctggtt atagtgcagt taaccactat
      541 aaccagcacc tcttatgttt tcttaaatca gcatcttaaa acagttaaga gcatactata
      601 aatcattatt tttcaatgcg ttagataaca atctacttta tcaaaattga ata
//
LOCUS       DAZGVQ010000454          641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000454 DAZGVQ010000000
VERSION     DAZGVQ010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.458"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>641
                     /locus_tag="ACHLAG_005506"
     CDS             <1..>641
                     /locus_tag="ACHLAG_005506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0658533.1"
                     /translation="DFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRVLSIDAATRTLT
                     LDREVTLPGTGTLTVNLINGSGKPVSVDITAHPAPDRIQVSPLPDGVETYGVWGLSLP
                     SLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGTLNSVIPPAVQH
                     LTVEVSAADSQYLALAKWDTPRVVKGVRFSLRLTSGSGENSRLLTTAITADTE"
BASE COUNT          133 a          195 c          201 g          112 t
ORIGIN      
        1 ggatttcaca ctcgggtcac aggggctgcg gcacacaccc ggtgacatta ttgaaatctg
       61 tgataatgac tatgccggga ccctgaccgg cggacgtgtc ctgtccattg atgctgccac
      121 ccgcaccctg acgctggacc gtgaagtgac acttccgggg acaggtacat tgacggtgaa
      181 cctgattaac ggcagcggta agccggtgag tgtggacatc accgcacacc ccgcgccgga
      241 ccggatacag gtcagcccct tgccggatgg cgtggagaca tacggggtgt ggggactctc
      301 cctgccgtca ctgcgccgtc gcctgttccg ctgtgtctcc atccgggaaa acacggacgg
      361 cacctttgcc atcacggcgg tgcagcacgt accggaaaaa gaagccattg tggataacgg
      421 agcccgcttt gagccgcagt caggcaccct gaacagcgtt atcccaccgg cagtacagca
      481 cctcacggtg gaggttagcg cagctgacag ccagtatctg gcgctggcga aatgggacac
      541 gccgagggtg gtgaagggcg tgcgcttcag tctgcgcctg accagtggaa gcggtgagaa
      601 cagccgcctg ctgaccaccg ccatcactgc cgatacggag c
//
LOCUS       DAZGVQ010000455          630 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000455 DAZGVQ010000000
VERSION     DAZGVQ010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.459"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..261)
                     /locus_tag="ACHLAG_005507"
     CDS             complement(<1..261)
                     /locus_tag="ACHLAG_005507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFP0658534.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKIN"
     gene            complement(304..540)
                     /gene="ycgZ"
                     /locus_tag="ACHLAG_005508"
     CDS             complement(304..540)
                     /gene="ycgZ"
                     /locus_tag="ACHLAG_005508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFP0658535.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
BASE COUNT          182 a          132 c          109 g          207 t
ORIGIN      
        1 gtttattttc ttaccattga acatactttt attgtcagct gtcgctgttc ttcggtttgg
       61 ccctcctgaa tcactattgc taaaatcacg aattatgctg tcaattaaat cgcgtcggaa
      121 agcatcttcc tcagccgatt tcattacgag taattgctta ataagagtct gcaatgaaaa
      181 atttatcttc atgcgcagta tctggatcac cgcctggcca gtaatttcat attttatacg
      241 ttcattatca gatgtcttca taacgacttc atagctaacc tgttaaataa tcaggctgtt
      301 gcattattca aaaagcaacc caattagtgc attataatgt ttctgttcct cttccccagt
      361 cgcgtgttcc agtcggcaaa gaagtttggc acaaagcgac tttcgactca gattacgtcc
      421 atctttcaaa atctcagtca caatttcccc gagagtttcc tgctgagtag gcaagttagc
      481 ttttgcaaag taacgagtga ttgctcccgc agaatctaaa gtcactgaat tttgatgcat
      541 gctacgcctc tgttaaaaat gttaatcaat gttaagtgag gtaacaatag caaacttgta
      601 cgaaatatgt gtacagaatg ataaattaat
//
LOCUS       DAZGVQ010000456          627 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000456 DAZGVQ010000000
VERSION     DAZGVQ010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.460"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(33..569)
                     /locus_tag="ACHLAG_005509"
     CDS             complement(33..569)
                     /locus_tag="ACHLAG_005509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP0658536.1"
                     /translation="MKRSIIAAAVFSYFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVA"
BASE COUNT          190 a          132 c          108 g          197 t
ORIGIN      
        1 cctggagaaa cttacaattt tatttattaa tcctacgcca cacgcataac cagagaagat
       61 aacacatcac ctgattgcac tgagttatct gtcagtttca ggtattttgc tttaaacgga
      121 atagcatatt gaccttccgc tgttttataa ctctcaatgt taaaaggtgc agaaacatcc
      181 aggacatcgc tgttatcacc taaatagaga tagtaaccca cggtatcgtt gctactggct
      241 atcgccttat cgttaccggt tattttatcg ttggtaatca acgagagttt tacaccagca
      301 acttctttcg ggcagctaat ttttaacatg ctgctggtta caccgactgc atcatcatag
      361 gtagaatatt tagctttacc ttcaaagaca gtggttggta cagtcggcat attaatactt
      421 actgaatcac cacccgcttc gaatttacac ggagattctg caatattccc cttaatagtt
      481 aatgttccgg tatcaacgtc tgcagcaaat actccagcgc tcataaaaaa ataagaaaag
      541 acagcggcag caataattga acgtttcata atatgttcct taataaaatt tatttttggt
      601 tgttaagagt ctgtatctct tggaaac
//
LOCUS       DAZGVQ010000457          625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000457 DAZGVQ010000000
VERSION     DAZGVQ010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.461"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>625
                     /locus_tag="ACHLAG_005510"
     CDS             <1..>625
                     /locus_tag="ACHLAG_005510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0658537.1"
                     /translation="NGKTTSQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLW
                     SSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYS
                     GIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQTVPDG
                     FGGTEPRMTFNAYLAQQRKVWDVLSDFCSAMRCMPVWNGQTLTFVQDR"
BASE COUNT          140 a          173 c          194 g          118 t
ORIGIN      
        1 ttaacggcaa gaccacctca cagttcctgg cctcggtgat tctggataat ctgcctcccc
       61 gcccctttaa catccggatg gtcagggaga cggcggacag caccacggac cagctgcaga
      121 ataagacgct ctggtcgtca tacaccgaaa tcatcgatgt aaaacagtgc tacccgaaca
      181 cggccattgt ggggctgcag gtggatgcgg agcagttcgg cggccagcag atgacggtga
      241 actaccatat ccgcggtcgc atcatccagg tgccgtcaaa ctatgacccg gaaaaacgca
      301 cgtacagtgg tatctgggac ggcagtctga aaccggcata cagcaacaac ccggcctggt
      361 gtctgtggga catgctgact cacccgcgct acggcatggg aaaacgtctg ggggcggcgg
      421 atgtggacaa gtgggcgctg tatgccatcg ggcagtactg cgaccagacg gtcccggatg
      481 gtttcggggg cacagagccg cggatgacct ttaatgcgta cctggcacaa cagcgtaagg
      541 tgtgggacgt tctcagtgat ttctgctcgg cgatgcgctg tatgccggta tggaacggcc
      601 agacgctgac gttcgttcag gaccg
//
LOCUS       DAZGVQ010000458          617 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000458 DAZGVQ010000000
VERSION     DAZGVQ010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.462"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..236
                     /locus_tag="ACHLAG_005511"
     CDS             <1..236
                     /locus_tag="ACHLAG_005511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP0658538.1"
                     /translation="FDHNWHTVVFRFAGNNSERVVPVIDGAEQTAFDLVMWTNDGFTA
                     DTLTLTDITGTKATYPVLLDTVTVKVNENRESA"
     gene            381..575
                     /locus_tag="ACHLAG_005512"
     CDS             381..575
                     /locus_tag="ACHLAG_005512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP0658539.1"
                     /translation="MTFVHQVMLYFCTVVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGAKTEAPLIPEQPS"
BASE COUNT          182 a          134 c          156 g          145 t
ORIGIN      
        1 cgtttgacca taactggcat acggttgttt tccgctttgc gggaaataac agcgaaagag
       61 ttgttccggt gattgatggt gcagagcaga cggcatttga ccttgtgatg tggacaaatg
      121 atggctttac agcagatacg ctgacgctga cagatatcac ggggacaaaa gcgacgtatc
      181 cggtactgct tgatacggtc acagtcaaag ttaacgaaaa ccgggaatca gcataaccgg
      241 caaaaaaaac cgccagctgg cctgaaaccc ctggcggtgt gagattcatg gtgagaatca
      301 gggaaagatg atatcacttt cgtcactggt attttttaaa cgaaaactgc ttccagagtc
      361 aaccataacg gtaagaaact atgacatttg ttcatcaggt gatgctgtac ttctgtacgg
      421 tggtatgcgt gctgtatctt ctttcgggtg ggtacagggc agtgcgcgat ttctggcgca
      481 ggcagattga taaaagggcc gcagagaaaa tcagcgccag tcagtcagcc ggagcaaaaa
      541 cagaagcccc actcattccg gaacaacctt cttaataacc cctttcaacg agaaaatcct
      601 atgtcagaaa taaaatc
//
LOCUS       DAZGVQ010000459          571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000459 DAZGVQ010000000
VERSION     DAZGVQ010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.463"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            4..>571
                     /locus_tag="ACHLAG_005513"
     CDS             4..>571
                     /locus_tag="ACHLAG_005513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP0658540.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNSAQKLINAGNQRLKLLTIAYCKNNSK"
BASE COUNT          180 a          107 c          114 g          170 t
ORIGIN      
        1 tatatggtgg cgaaaattaa tctcttatca cggcttacgc cgcttttatt cgtgttcgct
       61 ccttttttgg cacagtcaaa tatgactgtt tatcctatgg ctgtttccat taatagtcag
      121 ggtgaaggca acgttcgcgt aatatctaaa agtaatgaag ttcagtacat caaggcgaca
      181 gtattccgta tcgataatcc atcgacgcct caggaaaatg aagttgagat taaatccggt
      241 gatgcaaatc atctggttgt tatgccacct aaattcgctt taccagccgg tagtagcaaa
      301 accgtacgtt ttgttgcgat ggaaccagag caaaaagaga aaaattatcg cgttaaattt
      361 gaagcggttc ccagtattga tgacgttgcc acagataaaa aagatctctc tatgcagtta
      421 acagttaact taatttgggg aatcgttgtt agtgttccac ctcagcaacc tatagctaag
      481 ttagaagtaa attctgccca aaaattaatt aatgcaggga atcaacgctt aaagctttta
      541 acgatcgctt attgtaaaaa taatagcaaa g
//
LOCUS       DAZGVQ010000460          568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000460 DAZGVQ010000000
VERSION     DAZGVQ010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.464"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>568
                     /locus_tag="ACHLAG_005514"
     CDS             <1..>568
                     /locus_tag="ACHLAG_005514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP0658541.1"
                     /translation="KDHITITDSLNRREVLHTQGEAGLKRVVKKEHADGSVTQSQFDA
                     VGRLKAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTSATGPDGLEL
                     RREYDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLSFT
                     DCSGYQTRYDHDRFGQMTAVHREEGLSQY"
BASE COUNT          140 a          168 c          173 g           87 t
ORIGIN      
        1 aaagaccaca tcaccatcac cgacagcctg aaccgccgtg aagtgctgca cacgcagggc
       61 gaagccgggc tgaagcgggt ggtgaaaaag gaacacgcgg acggcagcgt cacgcagagt
      121 cagtttgatg cggtgggcag gcttaaggca cagacggatg ccgcaggcag gacaacagag
      181 tacagcccgg atgtggtgac gggactcatc acgcgcatca ccacgccgga tggcagggca
      241 tcggcgtttt actataacca ccacagccag ttaacgtcag ccaccgggcc tgacgggctg
      301 gaattgcgcc gggaatatga tgaatccggc cgtctgattc aggaaactgc ccctgacggc
      361 gatatcaccc gctaccgtta tgataatcca cacagtgact taccctgcgc aacggaagat
      421 gccaccggca gccggaaaac catgacgtgg agccgttacg gccagttgct gagcttcacc
      481 gactgttccg gctaccagac ccgttatgac catgaccgct tcgggcagat gacggcggtg
      541 caccgcgagg aagggctgag ccagtacc
//
LOCUS       DAZGVQ010000461          568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000461 DAZGVQ010000000
VERSION     DAZGVQ010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.465"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>568
                     /locus_tag="ACHLAG_005515"
     CDS             <1..>568
                     /locus_tag="ACHLAG_005515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP0658542.1"
                     /translation="KDRITITDSLNRREVLHTQGEAGLKRVVKKEHADGSVTQSQFDA
                     VGRLKAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHNQLTSATGPDGLEL
                     RREYDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQLLTFT
                     DCSGYVTRYDHDRFGQVTAVHREEGLSQY"
BASE COUNT          137 a          168 c          171 g           92 t
ORIGIN      
        1 aaagaccgca tcaccatcac cgacagcctg aaccgccgtg aagtgctgca cacgcagggc
       61 gaagccgggc tgaagcgggt ggtgaaaaag gaacacgcgg acggcagcgt cacgcagagt
      121 cagtttgacg cggtgggcag gcttaaggca cagacggatg ccgcaggccg gacaacagag
      181 tacagcccgg atgtggtgac gggcctcatc acgcgcatca ccacgccgga tggcagggca
      241 tcggcgtttt actataacca ccacaaccag ttaacgtcag ccaccgggcc tgacgggctg
      301 gaattgcgcc gggaatatga tgaatccggc cgtctgattc aggaaaccgc ccctgacggc
      361 gatatcaccc gctaccgtta tgataatcca cacagtgact taccctgcgc aacggaagat
      421 gccaccggca gccggaaaac catgacgtgg agccgttacg gtcagttgct gaccttcact
      481 gactgttccg gttatgtaac ccgctatgac catgaccgtt ttggtcaggt gacggcggtg
      541 caccgcgagg aaggcctgag ccagtacc
//
LOCUS       DAZGVQ010000462          563 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000462 DAZGVQ010000000
VERSION     DAZGVQ010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.466"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>563
                     /gene="ygeH"
                     /locus_tag="ACHLAG_005516"
     CDS             <1..>563
                     /gene="ygeH"
                     /locus_tag="ACHLAG_005516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFP0658543.1"
                     /translation="DMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRK
                     GYRFSGQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYT
                     YPMAATNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNH
                     LPVDELHNTSQFIIDNILQTVHKPERSV"
BASE COUNT          194 a          106 c           86 g          177 t
ORIGIN      
        1 gatatgatca tcgaatcagt atggaaaaat attattgtta gtgacgagtc cctgacaaga
       61 tgtatctatt ctttgcgctg catttttgaa aaaattggct atgatcgttg catagaaaca
      121 atctaccgga aaggttatcg tttcagcggg caggttttca aaactaaaat taatgaagat
      181 aatacttcag actattccat agctatattc cctttcacta cttcgttgaa tacactggat
      241 ccattaatac ttaatcagga attagtgcaa atcatttcaa ataaaaaaat cgatggtctc
      301 tatacctatc cgatggctgc gacaaatttt tgtaatgatc acatatctca aaattcattc
      361 ttgagcagat tcaagccaga ttatttcgtt acaggaagaa taaaccagaa taatgcagtg
      421 aacactttat acattgagtt gatcgacgct aaaaaccttt tcctcatcgc cagtaatcat
      481 ctccctgttg atgaactaca taatacatca caatttatta tagataatat ccttcaaacg
      541 gtacataaac cagaacgatc tgt
//
LOCUS       DAZGVQ010000463          538 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000463 DAZGVQ010000000
VERSION     DAZGVQ010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.467"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..471)
                     /locus_tag="ACHLAG_005517"
     CDS             complement(<1..471)
                     /locus_tag="ACHLAG_005517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFP0658544.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTFRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIISTKQHFFSSKLFKYVMLFFFFLL"
BASE COUNT          182 a          104 c           79 g          173 t
ORIGIN      
        1 tagcaaaaag aaaaaaaaaa gcattacata cttaaacagc ttactgctaa agaaatgttg
       61 ttttgtagaa ataatgttat tttcaataat gcctggcaat gaatactgcc aaacttcatt
      121 tttttctttt acagcaaatt tgagacctaa aacttctatt tgctgattca atgttaacgt
      181 tctatcttca ggttcttgtg ctgttaattc tctgaatgtt actaccagat cagggtcggt
      241 agtaattatt atctcaaaac tacctgtaag ctcgccataa ggaacgacta ttgtgttttc
      301 tggtaactca gaaaaagcat cacttcttcc taacgaaaca tcattaccaa tgataacaag
      361 tagacgccca ttaagtattt catactcgca tccattcaat ggtccgctca acaaacgaat
      421 ggcataagat tccaataagt cttgtgataa gaatttatca ttattttcca tgaataccac
      481 ccgctaattc cataagctct agtttagagt agtgtaacaa gacggaaatt tcatgacc
//
LOCUS       DAZGVQ010000464          533 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000464 DAZGVQ010000000
VERSION     DAZGVQ010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.468"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(70..>533)
                     /locus_tag="ACHLAG_005518"
     CDS             complement(70..>533)
                     /locus_tag="ACHLAG_005518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP0658545.1"
                     /translation="VATCMTITDDSKPLKNYASRDYHHNKRTILRVGTVEIICKRESI
                     LKTDLFPEDMGAGALFPLCDEPVFLNNCLKQSLKVQFLSIPLCYHPNVSSGSNLNRCY
                     TIARGVCFRRIYGMPALLLILIFSLKMKLKYRKLDLLTYTKSLYRGYNEGK"
BASE COUNT          197 a          100 c           80 g          156 t
ORIGIN      
        1 gaaaagaaag gccaaagtga caaaaagcac agaaatccat agaaccaatt aagatatggt
       61 gtcaaaacgc tacttgcctt cattataccc ccgataaaga gatttggtat aagttagcaa
      121 gtctaatttc ctgtacttca gtttcatttt caaagaaaat attaagatca atagcaatgc
      181 aggcatgcca tatatacgtc tgaaacaaac acctcttgca atggtatagc atctatttaa
      241 attactacct gaagaaacat taggatgata acaaagaggt atacttaaaa actgaacctt
      301 gagtgattgt tttaaacaat tatttaaaaa aacaggctca tcacacagag gaaataaagc
      361 accagccccc atgtcctctg gaaataaatc agtcttcaaa atactttcac gtttacagat
      421 tatctctact gtaccaactc ttaatattgt tcttttatta tggtgatagt cccttgatgc
      481 ataatttttc aaaggtttgc tatcatcagt aatagtcata caagtcgcga cat
//
LOCUS       DAZGVQ010000465          529 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000465 DAZGVQ010000000
VERSION     DAZGVQ010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.469"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..390
                     /locus_tag="ACHLAG_005519"
     CDS             <1..390
                     /locus_tag="ACHLAG_005519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFP0658546.1"
                     /translation="GTVGCGSTGNVENRLYTQEEVAGRWINDMRRAENCITQNFRGQQ
                     MPQSAFEAMTDAAFNLGCRNLMWFKNKNGTPQRTTIWKHAQTRQWRLMCYRLTDFVNS
                     GGTYTQGLVNRRNDFKNWCLKDAGAQP"
BASE COUNT          138 a          136 c          159 g           96 t
ORIGIN      
        1 ggtacggtcg gatgcggatc caccggtaac gtggaaaacc gcctgtacac acaggaggaa
       61 gtggccgggc ggtggatcaa cgatatgcgc cgcgctgaga actgcatcac acagaatttc
      121 agggggcagc agatgccgca gtctgcattc gaagccatga cggacgccgc ctttaacctg
      181 ggctgccgga atctgatgtg gtttaaaaac aaaaacggca cgccgcaacg caccaccatc
      241 tggaaacacg cgcagacacg gcaatggcgc ctcatgtgtt accggctgac agattttgtg
      301 aacagtggcg gaacgtacac gcagggactg gttaaccgcc ggaatgattt taagaactgg
      361 tgcctgaaag acgcgggagc gcagccatga aactgaacat tatcctttgt gcgctgctgc
      421 tgactatttc gctgcaggca atggtggtca tccgggccta tcgctcaggt caggagagca
      481 cccgttatga agcgctggaa aaagccgtga aagagagcaa tacgaccgt
//
LOCUS       DAZGVQ010000466          526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000466 DAZGVQ010000000
VERSION     DAZGVQ010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.470"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..74)
                     /locus_tag="ACHLAG_005520"
     CDS             complement(<1..74)
                     /locus_tag="ACHLAG_005520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658547.1"
                     /translation="MSAITVSRPEVVNGHTDVICSTSIR"
     gene            complement(159..356)
                     /locus_tag="ACHLAG_005521"
     CDS             complement(159..356)
                     /locus_tag="ACHLAG_005521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP0658548.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQLPDQNR"
     gene            complement(368..>526)
                     /locus_tag="ACHLAG_005522"
     CDS             complement(368..>526)
                     /locus_tag="ACHLAG_005522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP0658549.1"
                     /translation="DAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDASAECL
                     PDLPTFDW"
BASE COUNT          127 a          127 c          143 g          129 t
ORIGIN      
        1 cggatactgg ttgagcagat aacgtccgta tgcccgttga ccacttccgg acgggagact
       61 gtgattgcag acataatcga tattcctttt cgcagataaa tgaaaacgcc agccctgttc
      121 aggctggcgt cagtggaatg aagtatgatg aatgagactc accggttctg gtctggaagc
      181 tgcagctggc gcagggtacg gatatcctcc ctggcctgtt caatacaggg caacagtgct
      241 gccgaatccg ttttatcttc atcgttgtcg aagataattc ccgattcgca gtcgatattg
      301 tcctgcagcc atgcaatcag aatatccagc gctgtttccg tggttaatga tttcatgttg
      361 tttctggtta ccagtcgaaa gtgggtaaat ccggcagaca ttcggcgctg gcatccagat
      421 gaatgagact gacgccataa cgtcgggtaa gtgtgacaac cagacggcgg aacgttctgg
      481 acaggcccag gcgtttaagc cgcagaaccg ggtatgacca cgcatc
//
LOCUS       DAZGVQ010000467          522 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000467 DAZGVQ010000000
VERSION     DAZGVQ010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.471"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>522
                     /gene="yfdF"
                     /locus_tag="ACHLAG_005523"
     CDS             <1..>522
                     /gene="yfdF"
                     /locus_tag="ACHLAG_005523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFP0658550.1"
                     /translation="EPIELPANYKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIY
                     DGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDY
                     LSLGYYDQDDYHIQRGIRINGDSLTQYCSENARSASAWFESSKAIMAESFATGSDHQV
                     VNELNGERLREPN"
BASE COUNT          193 a           96 c          108 g          125 t
ORIGIN      
        1 aagaaccaat agaattacct gcgaactata aagacaaaga actcgtccgt actatcatta
       61 atgacaacat agttgagaag actcatgata tcaataacaa ggaaatgatc ttcagcgcat
      121 taaaagaaat atatgatgga gatcctggtt ttatcttcga taagatatca cacaaactca
      181 gacatacggt aacggaattt gatgagagcg ggaatagcga accaacggac ttatttacct
      241 ggtacggtaa agataaaaaa ggcgactctc tcgctattgt aattaaaaat aaaaacggaa
      301 atgattactt atctctcggt tactacgatc aggacgacta ccacattcaa agaggaattc
      361 gtattaatgg tgatagtctc acccaatatt gtagtgaaaa cgccaggagt gcttcagcgt
      421 ggtttgaaag cagcaaagct atcatggcag aatcatttgc aacaggttcc gatcatcagg
      481 ttgtaaacga gctcaacggg gaaagactga gagaacctaa cg
//
LOCUS       DAZGVQ010000468          519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000468 DAZGVQ010000000
VERSION     DAZGVQ010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.472"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>519
                     /locus_tag="ACHLAG_005524"
     CDS             <1..>519
                     /locus_tag="ACHLAG_005524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFP0658551.1"
                     /translation="DFYRINRFGRLEKYYYIQLRGAFLSAIHHQIIENQLDTETITIS
                     YEFILCQHLIANTEFSYLALPENYNRLFLPNSKNQTNNRFKTLNSKAIGRLLAAGGVY
                     NGNIEGFRDTAEKLGGDAIKGYDQILNEKTAGIAIATASILLTKRSNVDTYTEINSYL
                     GKLRGQQKLLDG"
BASE COUNT          193 a           88 c           91 g          147 t
ORIGIN      
        1 tgacttctat cgaataaata gatttggtag attggaaaag tattattata tacaactaag
       61 aggcgctttt ttatcggcta ttcatcacca gatcattgaa aaccaactgg atacagaaac
      121 aataactatt agttatgaat ttatcctctg tcaacatctt atcgcaaata ccgagttcag
      181 ctatttggca ctccctgaaa attataaccg tttgttttta ccaaattcaa aaaaccaaac
      241 aaataatcgt ttcaaaacgt taaacagcaa agctattggc aggctacttg ctgctggtgg
      301 cgtatacaat gggaacattg aaggattcag agatactgcg gaaaaactgg gtggagatgc
      361 aataaaaggc tatgatcaaa tactaaatga aaaaacagcg ggcatagcga tagcaacagc
      421 atctattctt ttaacaaagc gttctaatgt tgatacatat acagaaataa atagttactt
      481 aggcaaactt agaggtcaac aaaaacttct tgatggtat
//
LOCUS       DAZGVQ010000469          511 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000469 DAZGVQ010000000
VERSION     DAZGVQ010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.473"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          151 a           96 c           78 g          186 t
ORIGIN      
        1 tattattaca tataaagaaa catttgtcaa taccttttca taaaaaatct gatctttttc
       61 atgcacttac attttttctt agcaattcgt gctctcctat gtagtgctat tcttcattat
      121 tgtctaaaac gctctaaaac gcgttctaac gaggttttga taaaagcaat acattccctt
      181 atctaatggc tgtttagctc gccagaggct tatttaaggg gcttattttg tatttgttag
      241 gtatatgaaa aggtgatggt agatataaga tagagagtca ctttataaaa acgctctgta
      301 aagctctgta gtgagcttat tatatgtagg caatgcgttt ccattcccta ccctgcttaa
      361 tgctgttaga gaagcattta gaccccttat ttttaattaa ttaaagtatc ctctatacac
      421 tcctgttatg aaacacctct gaatccttca taaaacacat tatgacgcaa agaacacctt
      481 ttctaaggtg attcccttcc tgaatgctgt t
//
LOCUS       DAZGVQ010000470          508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000470 DAZGVQ010000000
VERSION     DAZGVQ010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.474"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>508
                     /locus_tag="ACHLAG_005525"
     CDS             <1..>508
                     /locus_tag="ACHLAG_005525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFP0658552.1"
                     /translation="LYGYQLNPISDIDPLGLSMWEDAKSGACTNGLCGTLSAMIGPDK
                     FDSIDSTAYDALNKINSQSICEDKEFAGLICKDNSGRYFSTAPNRGERKGSYPFNSPC
                     PNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKIVKSKDNNIKSFYLGTPSGNFKAIDN
                     HGKEITNRK"
BASE COUNT          184 a           83 c          107 g          134 t
ORIGIN      
        1 ctgtatggat atcaattgaa tccgatatca gacatcgacc ccctgggttt atctatgtgg
       61 gaggatgcaa aatcgggggc atgtactaat ggtctttgcg gcacactatc cgctatgata
      121 ggtccagata aatttgattc tatagatagc accgcatatg acgccttaaa taaaataaat
      181 agccaatcta tttgcgaaga taaagagttc gctggtttaa tatgtaagga taatagtggc
      241 agatatttct caacagcacc taaccgagga gaaagaaaag gatcatatcc attcaatagc
      301 ccttgcccta atggtactga gaaagtatca gcttatcata ctcatggtgc agatagtcat
      361 ggagaatatt gggacgaaat attttcaggt aaagatgaga aaatagttaa aagtaaagat
      421 aacaatatca agtcatttta tttaggtacg cccagtggta attttaaagc aatagataac
      481 cacgggaagg aaataacaaa cagaaaag
//
LOCUS       DAZGVQ010000471          494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000471 DAZGVQ010000000
VERSION     DAZGVQ010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.475"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..286
                     /locus_tag="ACHLAG_005526"
     CDS             <1..286
                     /locus_tag="ACHLAG_005526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658553.1"
                     /translation="VRVIFEFDHVMHEVKPAGNDSEEITEGVTATVKIERDTENRPAG
                     PCDVYAQILKYHSPTIIQFLTDELQGSMQAMGVSSSVERRSVQNGPDTLQ"
     gene            298..>494
                     /locus_tag="ACHLAG_005527"
     CDS             298..>494
                     /locus_tag="ACHLAG_005527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0042262 - DNA protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP0658554.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKEI"
BASE COUNT          162 a          112 c          126 g           94 t
ORIGIN      
        1 agtacgcgtt atttttgaat ttgaccacgt aatgcatgaa gtaaagccag ccggtaatga
       61 tagtgaggaa atcacggagg gggttactgc cacggtaaaa atcgaacgtg atacagagaa
      121 cagaccggca ggaccctgtg atgtttatgc acaaattctt aaatatcaca gccccacaat
      181 cattcagttt ctgacagatg aactacaggg ttccatgcag gccatggggg taagcagctc
      241 cgttgaacgc cgttctgttc agaacggacc agatacattg caataaggaa aaacaaaatg
      301 gcaaaacgcg ttacaaaatt aaaggccgca gcagaggcgg caccgcagac ccgtgaagag
      361 gtcagccgcg atatccgcac cctgggcgat attcagcgag aggcgctgcg cctggaaaca
      421 gcgatgaatg atgaagtggc agaaatcacc gcccgttata cgccgcagat tgaaaacctt
      481 aaaaaagaaa tcaa
//
LOCUS       DAZGVQ010000472          490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000472 DAZGVQ010000000
VERSION     DAZGVQ010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.476"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..490
                     /locus_tag="ACHLAG_005528"
                     /pseudo
     CDS             1..490
                     /locus_tag="ACHLAG_005528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
BASE COUNT          140 a          126 c          132 g           92 t
ORIGIN      
        1 atgctggcgt ggctaaactc gccacgctgt cagatggcac agtctttgag cctgtaaaca
       61 gttttcagaa aaactagaag acgccggcga gacttcagcg acagttaagc cgcagggtca
      121 aattcagcaa caactggcag aagcagaaac gcaaaataca gcgactgcat tcctgtatcg
      181 caaatatccg cagggactac cttcacaaag tcacaacgac cgtcagcaaa aaccacgcaa
      241 tgatagtcat tgaggatttg aaggtcagca acatgtcaaa gtcagcagcg ggtacggtca
      301 gccagccggg gcgcaatgtc cgggcaaaat caggtttaaa ccgttcgata ctggatcagg
      361 gctggtatga aatgcgccgc cagcttgagt acaagcagct ctggcgtggc ggtcaggtgc
      421 ttgctgttcc gccagcgtac acaagccagc gttgcgcgtg ctgtggtcat acagcgaaag
      481 aaaaccgcct
//
LOCUS       DAZGVQ010000473          487 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000473 DAZGVQ010000000
VERSION     DAZGVQ010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.477"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          163 a           81 c          121 g          122 t
ORIGIN      
        1 tattaataat aaagtagata aagtaagtaa gataactaag atagataaga ttaataagat
       61 cagaattctc tttcggtgtc gcctctggct tttccaccga aaacaattac acgactacag
      121 aatgtaggaa gtggaattgt attgaggctt agtgaaatta cacgaggaaa gcagagtgta
      181 attgagcttt agccgctctc tcttcgagta attacacaac tacagagaat aacagagtta
      241 ttatatgtag gaagtggaat taagaggtag agagagagag ttaacacata agcaatctgt
      301 agattggtta ggtgattact cttactgcgc acagcgcaat agtatttctt cgtgggcgtt
      361 accgccaacg atagttacac gaagaaagta acgctttgct ttcggtgtga gcggaacggg
      421 ccagccaacg tagcccgaaa ggtagcggag taaggctgct tgccagagag tgaagctaac
      481 gccggaa
//
LOCUS       DAZGVQ010000474          487 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000474 DAZGVQ010000000
VERSION     DAZGVQ010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..487
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.478"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>487
                     /locus_tag="ACHLAG_005529"
     CDS             <1..>487
                     /locus_tag="ACHLAG_005529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP0658555.1"
                     /translation="LAGKKELYDFTPESIYRAMTIFDRLQNKSDIQTLKTECYCLLAE
                     CHMSLALHGKSELELAAQKALELLDYVSDITTVDGKILAIMGLITGLSGQAKVSHILF
                     EQAKIHSTDIASLYYYRALVHFHNEKIEEARICIDKSLQLEPRRRKAVVIKECVDMYV
                     P"
BASE COUNT          175 a           86 c           92 g          134 t
ORIGIN      
        1 tgttagccgg aaagaaagaa ctttacgact tcacccctga aagcatttac agggccatga
       61 ctatatttga tagattacaa aataaaagtg atatacagac gctaaaaaca gaatgttatt
      121 gccttctagc ggaatgccat atgtctttgg cacttcatgg aaaaagtgaa cttgaacttg
      181 ctgctcaaaa agcattagag cttttagatt atgtatcaga cataaccact gtcgatggaa
      241 aaattttagc tattatggga ctgataactg gtctgtctgg acaagcaaaa gtatctcata
      301 tcttatttga acaggctaag atacactcaa ctgatatagc ctctctctac tactataggg
      361 cactagtcca ctttcataat gaaaaaattg aagaggcaag gatttgtata gacaaatcac
      421 tacaactcga acccagaaga cgaaaagcag ttgtgataaa agaatgtgta gatatgtatg
      481 tgcctaa
//
LOCUS       DAZGVQ010000475          485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000475 DAZGVQ010000000
VERSION     DAZGVQ010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.479"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            151..312
                     /gene="rhuM"
                     /locus_tag="ACHLAG_005530"
                     /pseudo
     CDS             151..312
                     /gene="rhuM"
                     /locus_tag="ACHLAG_005530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774140.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
BASE COUNT          129 a          120 c           75 g          161 t
ORIGIN      
        1 tttatccaac acagattaat catttaaaat caatatatta atactcatat tcgactcctg
       61 tgatctccgc caaaatgcct ctcctgacgt tctccaaccc cttgtaatac tgtcaatcca
      121 gatattttct actgctcaat attcaatcgg cttcaggaat acctggtcta aggttttgtg
      181 atggacgatg agcggctgaa gaatccgcct gtcggttctt ctgtcctcga acgcatccgc
      241 gatattcacg ccagtatgcg ccgtgtttat ttgcaggtga tttgcccccc tgctgactct
      301 cagccaactg tatcgccatt aaggtgaaga tgttgtttta ctcgccccag aacacccttt
      361 tttatacgac tattctattt ctttaaccgc tggcgaaaaa aactgactat aaacttcacc
      421 aataatcaga ctgccatcaa gattaatttg ttcatttaat atatcaaatc aacttgtttt
      481 atttt
//
LOCUS       DAZGVQ010000476          478 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000476 DAZGVQ010000000
VERSION     DAZGVQ010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.480"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>478
                     /locus_tag="ACHLAG_005531"
     CDS             <1..>478
                     /locus_tag="ACHLAG_005531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HFP0658556.1"
                     /translation="KLEIKVAHIRTRETYGTRRLQTELAENGIIVGRDRLARLRKELR
                     LRCKQKRKFRATTNSNHNLPVAPNLLNQTFAPTAPNQVWVADLTYVATQEGWLYLAGI
                     KDVYTCEIVGYAMGERMTKELTGKALFMALRSQRPPAGLIHHSDRGSQYCAYDYRVI"
BASE COUNT          125 a          128 c          132 g           93 t
ORIGIN      
        1 aaaactggag attaaggtgg cacatatccg cactcgcgaa acatatggaa cccggcggct
       61 ccagacggag ctggcagaga atggcatcat cgttggtcgt gaccgactgg cacgtcttcg
      121 taaggagcta aggctacgct gtaagcagaa acgcaagttc agagcgacta cgaactcgaa
      181 ccacaatctg ccagttgcgc caaatctgct gaaccagacg ttcgctccta cagcaccaaa
      241 tcaggtctgg gtggcggacc tgacgtatgt tgccacacag gagggatggt tgtacctcgc
      301 tggcatcaaa gatgtttata cgtgcgaaat tgtcggctac gccatgggag agcgcatgac
      361 aaaagagctg acaggtaaag ccctgtttat ggcgctcagg agccagcgcc cacctgccgg
      421 gctaatccac cactccgatc gtggttcaca gtactgcgca tacgattacc gggtcata
//
LOCUS       DAZGVQ010000477          474 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000477 DAZGVQ010000000
VERSION     DAZGVQ010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.481"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(223..456)
                     /gene="ydfK"
                     /locus_tag="ACHLAG_005532"
     CDS             complement(223..456)
                     /gene="ydfK"
                     /locus_tag="ACHLAG_005532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFP0658557.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNKNLNGKM"
BASE COUNT          136 a           75 c           92 g          171 t
ORIGIN      
        1 aaataaacga atgcgtatgc aaccccgtaa ttttggtgag acccaaaatc gattttgtga
       61 aaaatggcct taactcggtt tgtttttcga gtcccggggg gactcaagga agaagaatag
      121 tgttgcgtgt tattttaacc agatttcaag ttgtttggtc gtgaaaaagt ggagcaaaat
      181 gttgttaaag tggaaaaatg ataaaaaagt aagtttatta tattacattt taccatttaa
      241 atttttgttg tctttaagaa ctgatatcgc tgtttgtaat aattctttgt tatccagcca
      301 tgactttttc tttatgtttc cttcaatgta atcaagcaat gttctggtat tgataggtct
      361 tccctgtttt gctacttcca ctacagcatc ccctaggata attcttactt caggaagctg
      421 cgcagggaac cactttaggg tgtcttttga tttcatgaag atattcctta aaat
//
LOCUS       DAZGVQ010000478          463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000478 DAZGVQ010000000
VERSION     DAZGVQ010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.482"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..313
                     /locus_tag="ACHLAG_005533"
     CDS             <1..313
                     /locus_tag="ACHLAG_005533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP0658558.1"
                     /translation="KNLLCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDDKPSRFE
                     ILIPLVQTLVRDNVKLNNDVYKELKKFMHDYDKTSPEMRKYLQSINESMFLMKNIAHQ
                     N"
BASE COUNT          179 a           85 c           73 g          126 t
ORIGIN      
        1 aaaaaatttg ctctgtgaag atattatgcc tggtcgatgg accactcaac ttgtcaataa
       61 gcacttaggt tacagataca caggagtatt caaaaccctg gcaagtattg atgacaaacc
      121 gtcccgtttc gaaattttga taccattggt acaaacttta gttcgcgaca atgtaaagct
      181 aaataatgat gtgtataaag aactgaaaaa gtttatgcac gattacgaca aaacatcccc
      241 ggaaatgaga aaataccttc agagcatcaa cgagtctatg ttccttatga aaaacatagc
      301 tcatcaaaat taaaaaatct caaaaaagaa gtaatttcag aatgaaatta actgtgatga
      361 ttattaattc actacagagg aataaaaaat aatctgaagc atactctgaa acatcctact
      421 ctctcactct aataatggca ccttgctctt cttataaaga agg
//
LOCUS       DAZGVQ010000479          457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000479 DAZGVQ010000000
VERSION     DAZGVQ010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.483"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>457)
                     /locus_tag="ACHLAG_005534"
     CDS             complement(<1..>457)
                     /locus_tag="ACHLAG_005534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP0658559.1"
                     /translation="EDKMSGKTTQRPGLKRALKKLKPGDTLMVWKLDRLGRSMRHLVA
                     LTDELQQQGINFRSLTDSIDTSTPMGRFFFHIMGALAEMERELIVERTRAGLAAARER
                     GRTGGRPRRLTQEQYEQVGRLLAGGVSRRQVALIFDIGLSTLYRYFPASV"
BASE COUNT          100 a          136 c          107 g          114 t
ORIGIN      
        1 aaacgctcgc aggaaaataa cgataaaggg ttgaaaggcc gatatcaaaa ataagtgcca
       61 cctgtcttcg gctgacaccc ccggccagta aacgcccgac ctgctcgtat tgttcctgcg
      121 tgagacgacg gggtctgccg ccggttcgtc ccctttcacg ggcagctgca agaccagccc
      181 gtgtccgttc gacaatcaat tcacgctcca tttctgccag cgcacccatt atgtgaaaaa
      241 agaagcgccc catgggggtt gacgtatcaa tgctgtcagt aagactacga aaatttatcc
      301 cctgttgctg taattcatct gtcagcgcaa caaggtggcg catactgcgc cccagcctgt
      361 ccagtttcca gaccatcagc gtgtcaccgg gttttagttt cttcagtgcc cgcttcagtc
      421 ccggccgctg tgttgttttt ccgctcattt tatcttc
//
LOCUS       DAZGVQ010000480          457 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000480 DAZGVQ010000000
VERSION     DAZGVQ010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.484"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>457
                     /locus_tag="ACHLAG_005535"
     CDS             <1..>457
                     /locus_tag="ACHLAG_005535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0658560.1"
                     /translation="SFRANLQGAWDDRENARLGLAAATLQSDMEKAGELAARDRAERE
                     ASQLKYTGEAQKAYERLLSPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYES
                     TLKKPKSSGVKVSAGERQEDRAHAALLALETELRTLEKHAGANEKISQQR"
BASE COUNT          122 a          110 c          159 g           66 t
ORIGIN      
        1 cctcgttccg ggccaacctt cagggtgcat gggatgaccg ggaaaatgcc cgtctggggc
       61 tggcggcggc aacgctgcag tcggatatgg aaaaagccgg tgaactggcg gcaagggaca
      121 gggctgagcg tgaggcgtca cagctgaagt ataccggaga ggcgcagaaa gcgtatgaac
      181 gcctgctgtc gccgctggag aaatataccg cccgtcagga agaactgaac aaggccctga
      241 aggacgggaa aatcctgcag gcggattaca acacgctgat ggcggcggcg aaaaaggatt
      301 atgaatcgac gctgaaaaag ccgaagtcgt caggtgttaa agtgtcagcc ggtgagcgtc
      361 aggaagaccg ggcgcatgcg gcgctgctgg cgcttgaaac cgagctcagg acgctggaaa
      421 agcatgccgg tgcgaatgaa aaaatcagcc agcagcg
//
LOCUS       DAZGVQ010000481          456 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000481 DAZGVQ010000000
VERSION     DAZGVQ010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.485"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(277..>456)
                     /locus_tag="ACHLAG_005536"
     CDS             complement(277..>456)
                     /locus_tag="ACHLAG_005536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0658561.1"
                     /translation="NNDVGVTAEELIALSEAAEQAMFTKGMEIHVRQRTMKKELEKLT
                     SADEILAYRVGWAQE"
BASE COUNT          126 a          114 c           75 g          141 t
ORIGIN      
        1 aggatattcc gaaaataaat ttaaaaatta gttttataaa tttaggattt gtcttacatt
       61 attctgggca tacctgctca cgcatacgac cacaccgccg gaacagatac cagaaaaaac
      121 ttaccccagt cagacttgct gtttcacatc tgcttacact gtaatttgtg aacaaaatta
      181 caacatcaga aaaaattaac tattcagtta acatgaaata tttacaggag gtatagttca
      241 tgagggaaag aaatgtttcc cccatatttt tatctgttat tcctgtgccc agccaaccct
      301 gtatgccagt atctcatccg cactggtcag cttttccagc tctttcttca tggtgcgctg
      361 ccggacgtgg atttccatcc ctttggtgaa catcgcctgc tctgccgctt cactcagcgc
      421 tataagctct tctgctgtca cccccacatc attgtt
//
LOCUS       DAZGVQ010000482          451 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000482 DAZGVQ010000000
VERSION     DAZGVQ010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..451
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.486"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>451
                     /locus_tag="ACHLAG_005537"
     CDS             <1..>451
                     /locus_tag="ACHLAG_005537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP0658562.1"
                     /translation="SFRANLQGAWDDRENARLGLAAATLQSDMEKAGELAARDRAERE
                     SSQLKYTGEAQKAYERLLTPLEKYTARQEELNKALKDGKILQADYNTLMASAKKDYES
                     TQKKPSGVKVSAGERQEDRAHAAMLALETELLTLEKHAGANEKISQQR"
BASE COUNT          121 a          112 c          152 g           66 t
ORIGIN      
        1 cctctttccg ggccaacctt cagggcgcat gggatgaccg ggaaaatgcc cgtctgggtc
       61 tggcagcggc aacgctgcag tcggatatgg aaaaagccgg tgaactggcg gcaagggaca
      121 gggctgagcg tgagtcgtca cagctgaagt ataccggaga ggcgcagaag gcgtatgagc
      181 gcctgctgac gccactggag aaatataccg cccggcagga agagctgaat aaggccctga
      241 aagacgggaa aatcctgcag gcggattaca acacgctgat ggcgtcggca aaaaaggatt
      301 atgaatcgac gcagaaaaag ccgtccggtg tgaaggtgtc tgccggtgag cgccaggaag
      361 accgggcgca tgcagccatg ctggcgcttg aaaccgagct cctgacgctg gaaaagcatg
      421 ccggtgcgaa tgaaaaaatc agccagcagc g
//
LOCUS       DAZGVQ010000483          447 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000483 DAZGVQ010000000
VERSION     DAZGVQ010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.487"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>447
                     /locus_tag="ACHLAG_005538"
     CDS             <1..>447
                     /locus_tag="ACHLAG_005538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP0658563.1"
                     /translation="IVALVHSHPGGLPRLSEADRRLQIKSALPWWLVCRGDIHKFRCV
                     PHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDEWWRNGQNLYLDNMEASGFYR
                     VPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHIPEQLSKRER"
BASE COUNT           85 a          120 c          145 g           97 t
ORIGIN      
        1 agattgtggc actggtccac agccatcccg gtggcctgcc caggctgagc gaggccgacc
       61 ggcggctgca gataaaaagt gccctgccct ggtggctggt ctgccggggg gacattcata
      121 aattccgctg tgtgccgcac ctgaccgggc gtcgttttga acacggggtg acggactgtt
      181 acacgctgtt cagggatgca taccatctgg cggggattga gatgccggat tttcaccgcg
      241 aggatgagtg gtggcgcaat ggccagaacc tgtatctgga caatatggag gcctccgggt
      301 tttaccgggt gcccctgtcc tcggcacagg caggcgatat cctgctgtgc tgctttggcg
      361 catcggtgcc gaatcatgcc gccatttact gtggcaacgg tgagctgctt caccatatac
      421 ctgaacaact gagtaaacgg gagaggt
//
LOCUS       DAZGVQ010000484          445 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000484 DAZGVQ010000000
VERSION     DAZGVQ010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..445
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.488"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..203
                     /locus_tag="ACHLAG_005539"
     CDS             <1..203
                     /locus_tag="ACHLAG_005539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP0658564.1"
                     /translation="AELEAREVQLPTRYDLRYGHPINADERHVMIPKENGSWLYLIDL
                     EHALRVAGIRIKGEEHGNKTRG"
     gene            181..>445
                     /locus_tag="ACHLAG_005540"
     CDS             181..>445
                     /locus_tag="ACHLAG_005540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658565.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVL
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRHLRQEE"
BASE COUNT          130 a           93 c          117 g          105 t
ORIGIN      
        1 ttgctgaact ggaagcgcgg gaagttcaat taccgactcg ctacgacctt cgatatggac
       61 acccgataaa tgcagatgag cgacatgtca tgatacctaa agaaaatggc agttggcttt
      121 acctgattga cctagaacac gcattacgcg tcgctggcat tcgcatcaaa ggagaggagc
      181 atggaaataa aaccagagga tgagttaagc aatatcgttt tatttccggt aaaagaggat
      241 gaccctcgta atcaggttaa ttttctttat gagccatcgg aaagaccata ttgtcatcac
      301 gcctctgtcc tggttgacga aaaagagcgt caggtccgct gtaaaatctg cggtgcagtt
      361 gtggagccat ttgactggat gctctctgtg gcgaaaagag aaaccagact ggcagatgat
      421 gtaaggcact tgcgccagga ggagc
//
LOCUS       DAZGVQ010000485          436 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000485 DAZGVQ010000000
VERSION     DAZGVQ010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.490"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(96..305)
                     /locus_tag="ACHLAG_005541"
     CDS             complement(96..305)
                     /locus_tag="ACHLAG_005541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFP0658566.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
BASE COUNT           92 a          146 c           98 g          100 t
ORIGIN      
        1 ctgtgaattt gccgaaattt tgataatcag ttcacgtaac gtcgccattc acctttctcc
       61 gggcgtaaaa aaaccgcctc agcggttctc atcattcatg actgtgctgc aaagctcagc
      121 gcgtcttcca gcgccgcaaa cggatccacc tccggcttat cctcatcctc gccccagcag
      181 agcatggcgt ccttcagtgc aacattcatc ccctgtgccc cgaaaaccgc tttcacgatc
      241 tgtgcattac ggatatcccc gcgctcatca cccagcgggg ataccctgtc gaactccatc
      301 cacatcatcg cctcgctcgc actcaggctg tggcgcagtt cggataaggt gcgccccaga
      361 cggagcgcaa gtcgcatcag aaagcgaatt tccgggcggg ctactttttt ctggccgact
      421 ctgcatcagc gatcag
//
LOCUS       DAZGVQ010000486          435 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000486 DAZGVQ010000000
VERSION     DAZGVQ010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.491"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(3..>435)
                     /locus_tag="ACHLAG_005542"
     CDS             complement(3..>435)
                     /locus_tag="ACHLAG_005542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP0658567.1"
                     /translation="QPERFFSAPGSFHYSNVRGESWQHITRQICAQLVAQHHITADEA
                     QRIIAREGEGENLIVNRLAIPHCWSEQERRFRGFFITLAQPVEVNNEVINHVLIACAA
                     ADARHELKIFSYLASILCQHPAEIIAGLTGYEAFMELLHKG"
BASE COUNT          109 a          106 c          108 g          112 t
ORIGIN      
        1 gttcacccct tgtgaagtaa ctccataaat gcctcatatc ctgttaaccc ggcaataatc
       61 tccgccggat gctgacacaa tatgcttgcc agatagctaa atatttttag ctcatgacgc
      121 gcatcggcgg cggcgcaggc gatcaagaca tggttaatga cttcgttatt cacctcaact
      181 ggttgggcga gggtaataaa aaatccacga aaacgtcgct cctgttcgct ccagcaatgt
      241 gggatggcga ggcgattaac aatcaggttt tcaccttcgc cttcgcgggc gatgatgcgt
      301 tgtgcttcat cggcggtaat atggtgttgt gcgactaatt gcgcacaaat ttgccgggta
      361 atatgttgcc agctttcacc gcgtacattc gaataatgaa aacttccggg ggcagagaaa
      421 aaacgctccg gttgc
//
LOCUS       DAZGVQ010000487          427 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000487 DAZGVQ010000000
VERSION     DAZGVQ010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.492"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>427)
                     /locus_tag="ACHLAG_005543"
     CDS             complement(<1..>427)
                     /locus_tag="ACHLAG_005543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP0658568.1"
                     /translation="TGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDTLP
                     GYTEYGRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSF
                     TYDDKYRGRMVAHRHTGRPEICYRYDSDGRVTEQLNPAG"
BASE COUNT           70 a          141 c          131 g           85 t
ORIGIN      
        1 aagcctgccg ggtttagctg ttctgtcacc cgcccgtcgc tgtcgtaacg gtaacagatt
       61 tccggtcggc ccgtgtgacg gtgcgccacc atccggcccc ggtatttatc atcgtaagta
      121 aagctgcgca cctgtgtatt gctgcggtca tacaccgccg ccagttcgcc gcgtggcgtc
      181 cagccatagc gcaccagcgg cgcggcaggt aaattctccg ggtattccgg gtcgtgcgtc
      241 agccacacgg cagacagacg gatgccgttg tcccggccat attcggtgta acccggcagg
      301 gtatcaggaa aagcggacgg ttccgtcccg ccggaaatgg cctgctgccg ggcttcttct
      361 gcccgctgcg cctgcgtggt cagtaccagc cggaagtgac gcccggcacc atccgtcacg
      421 ccggtga
//
LOCUS       DAZGVQ010000488          425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000488 DAZGVQ010000000
VERSION     DAZGVQ010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.493"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..366
                     /locus_tag="ACHLAG_005544"
     CDS             <1..366
                     /locus_tag="ACHLAG_005544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFP0658569.1"
                     /translation="EEWMISQMSFSGAILWINIFSSVLLVPVYEEIVFRGCLFNSFKF
                     WFNDNIYISAIVTSVIFSALHLQYTDFRTFLMLFLISLVLISAKIKSNGLLMPILLHM
                     AMNAVITGIQYTLQYHITV"
BASE COUNT          129 a           71 c           67 g          158 t
ORIGIN      
        1 gaagaatgga tgatctcgca aatgtctttt tccggggcca ttttatggat taatatattt
       61 tcttccgtac ttcttgttcc tgtatatgaa gagattgtat ttcgagggtg tctttttaac
      121 tcttttaaat tctggtttaa tgacaatatt tacatctctg ctattgtaac atcagtcata
      181 ttttctgcat tacatctaca atatactgac tttcgtacat ttttgatgct ctttctgata
      241 tcgttagttc ttatcagtgc aaaaataaag tcgaatggcc tgttaatgcc catattactt
      301 cacatggcga tgaatgcagt cataacagga atacaatata cgttacaata tcacataaca
      361 gtctgaaatt gttattatga gatatctata acacctgaag ttggaaaaag aagcaaataa
      421 ttcct
//
LOCUS       DAZGVQ010000489          406 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000489 DAZGVQ010000000
VERSION     DAZGVQ010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.494"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>406
                     /locus_tag="ACHLAG_005545"
     CDS             <1..>406
                     /locus_tag="ACHLAG_005545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658570.1"
                     /translation="FSSNHQDKHSDKVNSHQHHGKVDKTHRAKIVEFDKLDNDSQIDN
                     DFGLHIIYFLQHGHWKVNDRSHQMEKVWFYNSEPSIDIQEYNRFADNTTDTFIFTIIP
                     DNNHVLKLSSPITVTVECKGGYYFINSSGDKSD"
BASE COUNT          141 a           69 c           69 g          127 t
ORIGIN      
        1 ttctcttcaa accatcaaga taagcattca gataaagtta acagtcatca acaccatggc
       61 aaggttgata aaacacatcg tgcaaagata gttgaatttg ataaactgga taatgattcg
      121 caaatagata acgattttgg cctgcatatc atctatttcc tgcaacatgg tcactggaag
      181 gtaaatgatc gtagtcacca gatggagaaa gtatggtttt ataatagtga accttctata
      241 gatattcaag aatataatag attcgccgac aatactactg atacgtttat ctttacaatt
      301 ataccagaca ataaccatgt gttaaaatta tcgtctccca ttaccgttac tgttgagtgt
      361 aagggcggtt attattttat taattcctct ggagataaat cagata
//
LOCUS       DAZGVQ010000490          401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000490 DAZGVQ010000000
VERSION     DAZGVQ010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.495"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..62)
                     /locus_tag="ACHLAG_005546"
     CDS             complement(<1..62)
                     /locus_tag="ACHLAG_005546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658571.1"
                     /translation="MGNRKQVTSRIISTPELIRY"
     gene            complement(151..216)
                     /locus_tag="ACHLAG_005547"
                     /pseudo
     CDS             complement(151..216)
                     /locus_tag="ACHLAG_005547"
                     /inference="COORDINATES: protein motif:HMM:NF021626.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
     gene            331..>401
                     /locus_tag="ACHLAG_005548"
     CDS             331..>401
                     /locus_tag="ACHLAG_005548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658572.1"
                     /translation="MIQGGDYLLKENEELGGENDHKM"
BASE COUNT          107 a           74 c           98 g          122 t
ORIGIN      
        1 ttatagcgaa taagttcagg cgtgctgatg atacgggatg tgacttgctt tctgttcccc
       61 acgacaggat ttccttgtgt atgtggggtg ctctgtggcg ctcggagatg tgatttgatt
      121 gagggtttta cagcgcggac attttacttc cagataactg aaactggccc gtgccagtag
      181 cttattgcag tgtcggcacc gtatgtttcg atacatggcc gcataacctc ctgctttgtt
      241 gttgatgcct ttctcatgca atcgatcgac aaaaacgatc gattcgattt gtttaattga
      301 tacaacaaat agattgaaaa gtcatcaaca atgattcagg gcggtgatta tttacttaaa
      361 gaaaacgaag agttaggcgg tgaaaacgat cataaaatga t
//
LOCUS       DAZGVQ010000491          393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000491 DAZGVQ010000000
VERSION     DAZGVQ010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.496"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>393)
                     /locus_tag="ACHLAG_005549"
     CDS             complement(<1..>393)
                     /locus_tag="ACHLAG_005549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP0658573.1"
                     /translation="FNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLN
                     VLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSR
                     SFLIAKAASPWSKEDLLKPVNSNQYRYC"
BASE COUNT          122 a           80 c           61 g          130 t
ORIGIN      
        1 cacaataccg atactgattg ctatttacag gtttaagtaa atcttcttta ctccacggag
       61 aagctgcttt ggcgatcaaa aaactacgtg acaccggata gtcagcccac tcatgccagg
      121 gttttgtagg tcccacatag tgtataaaca cggtgtcatc attgactgga ttatcaactt
      181 tgtcttttaa ttcatagtta atactatatc gggtattata tttctccgaa ataaacttca
      241 ctttaccatt caataataca ttcagtacat cttgatcaag gtgggtgatt ttacttaccc
      301 aatctgggtc acgcaacatt tcaatagctt ttgacgaaat gttatttaga ttccactcat
      361 caatatttat cagtaaaaaa ccagcattga aat
//
LOCUS       DAZGVQ010000492          388 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000492 DAZGVQ010000000
VERSION     DAZGVQ010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.497"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>388)
                     /locus_tag="ACHLAG_005550"
     CDS             complement(<1..>388)
                     /locus_tag="ACHLAG_005550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP0658574.1"
                     /translation="ADRIIGDIVKGYTVDMGQGVMTQNQLTFQKMILLKDNMPFDRKL
                     MLMAYSFSRGEFIMKINDKVVVHARSGKVKTGEREVQISKNASRYEPVYSDVGGSASG
                     SWSIPAGQGDVRVEITFRYEADVLFYM"
BASE COUNT          102 a          109 c           70 g          107 t
ORIGIN      
        1 ccatgtaaaa aagcacatct gcttcatacc tgaatgtaat ttcaacccgc acatcaccct
       61 gtccggcagg aatactccat gagcctgacg cacttccgcc aacatcagaa tataccggct
      121 catatctgct ggcgtttttg gaaatctgga cctcccgctc cccggttttg actttgcctg
      181 acctggcatg aacgacaact ttgtcattta tcttcatgat gaattcacct ctggaaaatg
      241 aatacgccat aagcataagc ttgcgatcaa acggcatgtt gtctttcagc aaaatcattt
      301 tctgaaaagt cagctggttc tgagtcatta cgccctgccc catatcaacc gtgtatccct
      361 tgacgatatc accaatgatt cggtcagc
//
LOCUS       DAZGVQ010000493          382 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000493 DAZGVQ010000000
VERSION     DAZGVQ010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.498"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>382
                     /locus_tag="ACHLAG_005551"
     rRNA            <1..>382
                     /locus_tag="ACHLAG_005551"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           87 a           83 c          128 g           84 t
ORIGIN      
        1 tggtgggtag tttgactggg gcggtctcct cctaaagagt aacggaggag cacgaaggtt
       61 ggctaatcct ggtcggacat caggaggtta gtgcaatggc ataagccagc ttgactgcga
      121 gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa
      181 gggccatcgc tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag
      241 ttcatatcga cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag
      301 taggtcccaa gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg
      361 tcgtgagaca gttcggtccc ta
//
LOCUS       DAZGVQ010000494          371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000494 DAZGVQ010000000
VERSION     DAZGVQ010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.499"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..281)
                     /locus_tag="ACHLAG_005552"
     CDS             complement(<1..281)
                     /locus_tag="ACHLAG_005552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP0658575.1"
                     /translation="MTEQQIRLVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYDGSDKYPWPQPVLYHICLEMRTRGIERQMT"
     gene            complement(274..>371)
                     /locus_tag="ACHLAG_005553"
     CDS             complement(274..>371)
                     /locus_tag="ACHLAG_005553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="primosomal protein I"
                     /protein_id="HFP0658576.1"
                     /translation="GAYGGGVFEPLDSEERGRTFEALDCPDWCDD"
BASE COUNT           80 a          124 c           87 g           80 t
ORIGIN      
        1 tcagtcatct gacgttcaat ccctctggtg cgcatctcga ggcaaatgtg gtacagcaca
       61 ggctgtggcc acgggtattt atcactcccg tcgtatcgcc aggattcatt gcgccagcgc
      121 cggtactctt ccatcactgc atccaccgta agaccaaatg gatttgcccc actctccgaa
      181 atcagtgcaa caaactcagc caggtccggg ggccacgttt cacccgcccg gcagcggtcc
      241 atgcactgac gacagaccag acggatttgc tgttcagtca tcgcaccaat ctgggcaatc
      301 cagagcttcg aaggtgcggc cccgttcttc tgagtccagc ggttcgaata cacctccccc
      361 ataagctccc a
//
LOCUS       DAZGVQ010000495          366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000495 DAZGVQ010000000
VERSION     DAZGVQ010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.500"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..278)
                     /locus_tag="ACHLAG_005554"
     CDS             complement(<1..278)
                     /locus_tag="ACHLAG_005554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP0658577.1"
                     /translation="MTSKWVQLSSMPGNFTVKVSGGTAAFLEAPFPPAETKGGMTFAD
                     CLISFNTRDCLWVRPVSGDPNVEITGAGIGAVIPLSADVAGTTEPSDW"
BASE COUNT          109 a           95 c           73 g           89 t
ORIGIN      
        1 ttccagtctg acggttcagt agtaccggca acatcagcac ttaacggaat gacagcacca
       61 atacccgctc cggtaatttc cacgttcgga tcaccggata ctggccttac ccacaggcaa
      121 tcccgtgtat taaaactgat gagacagtca gcaaatgtca tacctccttt cgtttcagcc
      181 ggaggaaagg gagcctcaag aaatgccgct gtaccaccgg aaactttaac ggtaaagttt
      241 ccgggcattg atgatagttg aacccatttg gacgtcataa tattcatcct gttcctgaat
      301 aaacagaatt atcatatcca cagaccgata aacgccattt tgcgttaatt cagtaaaggc
      361 ccaaca
//
LOCUS       DAZGVQ010000496          363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000496 DAZGVQ010000000
VERSION     DAZGVQ010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.501"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            121..>363
                     /gene="dinJ"
                     /locus_tag="ACHLAG_005555"
     CDS             121..>363
                     /gene="dinJ"
                     /locus_tag="ACHLAG_005555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFP0658578.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLF"
BASE COUNT          109 a           79 c           79 g           96 t
ORIGIN      
        1 aaatatattt cagttaatta tgtaaaaaca tccgtttatt aaattactca tggattgtcg
       61 ctgttgctca tttgagctac aattcaagct gaataaatat acagcacagg agatacccca
      121 atggctgcta acgcatttgt tcgcgcccga atcgatgaag atctgaagaa tcaggcggcg
      181 gacgtactgg ccgggatggg gctgaccatc tctgacctgg ttcgcataac cctcacaaag
      241 gtcgcgcgtg aaaaggcatt gccgtttgat ttacgcgagc ctaatcaatt aaccattcaa
      301 tcaatcaaaa acagcgaagc tggcgttgat gttcataagg ccaaagacgc cgatgattta
      361 ttt
//
LOCUS       DAZGVQ010000497          362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000497 DAZGVQ010000000
VERSION     DAZGVQ010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.502"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(149..>362)
                     /locus_tag="ACHLAG_005556"
     CDS             complement(149..>362)
                     /locus_tag="ACHLAG_005556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFP0658579.1"
                     /translation="RKQQRARAERLHIRSEINRRLFRATRVAQIMHINMLHERTHALS
                     NRYCAAVFSYLAEDLAELQSLINQHR"
BASE COUNT           71 a           86 c          118 g           87 t
ORIGIN      
        1 cgggaagcca gcaggggatc caccgcccac atccagttaa agaagtcctg aatatctgaa
       61 agtcccttaa cgctcttcat gagaacctcc ggtaacagat gtgcggaagc ctcccgcgtg
      121 caggtgtggg atagcttccg gatgtggttt atcggtgctg gttgatgagg ctttgcagtt
      181 ctgccaggtc ttcggccaga tagctgaaga cggcagcgca gtaacggttt gatagtgcgt
      241 gcgtgcgctc atgcagcatg ttgatatgca tgatttgcgc gacgcgtgtt gcgcggaaaa
      301 gtctgcggtt gatttcgctg cggatgtgaa gacgctcagc gcgggcgcgc tgttgcttgc
      361 gg
//
LOCUS       DAZGVQ010000498          360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000498 DAZGVQ010000000
VERSION     DAZGVQ010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.503"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>360
                     /locus_tag="ACHLAG_005557"
     CDS             <1..>360
                     /locus_tag="ACHLAG_005557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP0658580.1"
                     /translation="VVVKPSDGEGTVISTTSEPEYYFVVVLAGQSNSMSFGEGLPLPE
                     TYDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSNLNHPHADLSKGQY
                     GCVGHALHVAKKLLPFMP"
BASE COUNT           80 a           86 c          109 g           85 t
ORIGIN      
        1 gtggtggtga agccgtcgga tggagaaggc acagttatca gcaccaccag cgaaccggag
       61 tattactttg ttgttgttct ggcggggcag tcaaactcaa tgtcttttgg tgaagggctg
      121 ccgctgccgg agacatatga ccgtccggac ccgcgtatta aacagctggc gcgtcgcagt
      181 acggtgacgc cgggtggtgc cgcctgtaaa tataacgaca tcattccggc agaccactgt
      241 ctgcatgatg tgcaggatat gagtaacctg aatcatcctc atgcagacct gagtaaggga
      301 cagtacggtt gtgtggggca tgctctgcat gttgccaaaa agctgctgcc gtttatgcct
//
LOCUS       DAZGVQ010000499          356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000499 DAZGVQ010000000
VERSION     DAZGVQ010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.504"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>356
                     /locus_tag="ACHLAG_005558"
     CDS             <1..>356
                     /locus_tag="ACHLAG_005558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HFP0658581.1"
                     /translation="RDDENKLVAKGDYGNPDAMVHDIRSSVLDEWHKDAPDLVVLMGR
                     DLFNTLRLPMINAISTTNPNTELVAGQLIVASRTVGGLQTYLAPYFPKDAMLITSLKN
                     LSIYFQKGSLRRFIRE"
BASE COUNT           82 a          101 c           94 g           79 t
ORIGIN      
        1 cgcgatgatg aaaacaaact ggtggcaaaa ggtgactatg gtaacccgga tgccatggtg
       61 cacgacatcc gctcatccgt gctggatgaa tggcataagg acgcaccgga tctggtcgta
      121 ctgatggggc gggatctgtt caatacactg cgtcttccca tgattaacgc catcagcacc
      181 accaacccga acactgagct tgttgccggg cagctgattg tggcgtcccg taccgtcggt
      241 ggtcttcaga cgtatcttgc gccgtacttc ccgaaagacg ccatgctcat cacgtcgctg
      301 aaaaacctgt cgatctactt ccagaaaggc tcactgcgcc gtttcattcg cgagga
//
LOCUS       DAZGVQ010000500          356 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000500 DAZGVQ010000000
VERSION     DAZGVQ010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..356
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.505"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>356
                     /locus_tag="ACHLAG_005559"
     CDS             <1..>356
                     /locus_tag="ACHLAG_005559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFP0658582.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEACDENTIGVVP"
BASE COUNT           81 a           94 c          106 g           75 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagcctgt gacgaaaaca ccatcggcgt ggtgcc
//
LOCUS       DAZGVQ010000501          355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000501 DAZGVQ010000000
VERSION     DAZGVQ010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.506"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            157..232
                     /locus_tag="ACHLAG_005560"
     tRNA            157..232
                     /locus_tag="ACHLAG_005560"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:191..193,aa:Glu,seq:ttc)
BASE COUNT          102 a           81 c           84 g           88 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgata gaaagtgaaa agcaaggcgt ttacgcgttg
       61 ggagtgaggc tgaagagaat aaggccgttc gctttctatt aatgaaagct caccctacac
      121 gaaaatatca cgcaacgcgt gataagcaat tttcgtgtcc ccttcgtcta gaggcccagg
      181 acaccgccct ttcacggcgg taacaggggt tcgaatcccc taggggacgc cacttgctgg
      241 tttgtgagtg aaagtcgccg accccaatat ctcaaaacag actgttaagt cttgtttgag
      301 atatttgctc tttaaaaatc tggatcaagc tgaaaattga aacactgaac aacga
//
LOCUS       DAZGVQ010000502          350 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000502 DAZGVQ010000000
VERSION     DAZGVQ010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.507"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            213..332
                     /locus_tag="ACHLAG_005561"
                     /pseudo
     CDS             213..332
                     /locus_tag="ACHLAG_005561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           82 a          102 c           66 g          100 t
ORIGIN      
        1 ggatcagatc tcgtctgatc gttccccctg cgctcactac catggctctt taccacagca
       61 gccggggttg tttgggaccg acttccgaca gcagatcccg gtgggcctac caccatctct
      121 tcacaagctt atgcttatta catgcaaaac gtcccgtttt gcatctttgt gcctacggca
      181 caccgatgat ctttccctgt gatctttccc tgaatctgcc cctgagaacc tgaatcaaac
      241 gtctgactcg cagaaccatc tgtttctcgc gctctgtaga acttcatgaa aaagtcatcg
      301 gtgcttttat cgaaaaatac atgttctact aattgaagtc atcatcaaaa
//
LOCUS       DAZGVQ010000503          349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000503 DAZGVQ010000000
VERSION     DAZGVQ010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.508"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            8..349
                     /locus_tag="ACHLAG_005562"
                     /pseudo
     CDS             8..349
                     /locus_tag="ACHLAG_005562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          110 a           71 c           67 g          101 t
ORIGIN      
        1 ataatcgggt acgattttat ccgataacta taatgctata actctgggaa atggttggaa
       61 tacaccgctg ggtgccatct ctttttacgc tgcccgatcg agtagtaagt taaataatga
      121 taccagacac gaaggtacga gctatcaggt cgcctataat aaatatctag tacaaacagc
      181 cactcgattt agcgtggccg cctggcgtta tgcttcgcag gattacagga cattcagcga
      241 ccatctttac gaaaatgata aacactatca tcagagtgat tataatgatt tttatgatat
      301 tggcagaaaa aatagccttt ctgccaatat tatgcaacct ttatccaat
//
LOCUS       DAZGVQ010000504          349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000504 DAZGVQ010000000
VERSION     DAZGVQ010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.509"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..349
                     /locus_tag="ACHLAG_005563"
                     /pseudo
     CDS             1..349
                     /locus_tag="ACHLAG_005563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          100 a           76 c           88 g           85 t
ORIGIN      
        1 caaagccgaa gctgtcagaa cggttcttga aaatcaactt tcgatcagtg aaggcgcttc
       61 ccgattatct cttcctgaag gcactttagg acaatgggtt accgccgcca gaaaagggct
      121 cggtactcct ggttcccgca cggtggctga actggaatct gaaattctgc aactgcgtaa
      181 ggcgttaaat gaagctcgcc ttgagcgaga tatattaaaa aaagcaacag cgtattttgc
      241 acaggagtcg ctgaaaaata cgcgttaatc gaacaatggc gacaacaatt tcccattgaa
      301 gcgatgtgtc aggtatttgg tgtatccagg agcggttatt acaactggg
//
LOCUS       DAZGVQ010000505          345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000505 DAZGVQ010000000
VERSION     DAZGVQ010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.510"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            152..>345
                     /locus_tag="ACHLAG_005564"
     CDS             152..>345
                     /locus_tag="ACHLAG_005564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFP0658583.1"
                     /translation="MLTPAQRYVQQAVARNRVTMNARETQRERTAHEQVLHQLRLAQM
                     QLKGIQSNVARAELKKHLLP"
BASE COUNT          100 a           95 c          103 g           47 t
ORIGIN      
        1 tgctacgtgg tggaagacta cggaaaatgc gcgctgattg aggggatcac cttcgcaggt
       61 gaagatccgg acaacaacgc cgaaagcgga cagccgcaac ccgaagcata acccggacgg
      121 gcgcgccgcg cccgttaaca ggagctgaga aatgctgaca ccggcacaac gctatgtgca
      181 acaggcggtg gcccgcaacc gggtgaccat gaacgcccgg gaaacacaac gggaaagaac
      241 cgcacatgaa caggtccttc accagttaag gctggcacag atgcagctga agggtattca
      301 gtcaaacgtc gccagggcag agcttaaaaa acatctgttg cctga
//
LOCUS       DAZGVQ010000506          344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000506 DAZGVQ010000000
VERSION     DAZGVQ010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.511"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>344
                     /locus_tag="ACHLAG_005565"
     CDS             <1..>344
                     /locus_tag="ACHLAG_005565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP0658584.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAZGVQ010000507          337 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000507 DAZGVQ010000000
VERSION     DAZGVQ010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.512"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>337
                     /locus_tag="ACHLAG_005566"
     CDS             <1..>337
                     /locus_tag="ACHLAG_005566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFP0658585.1"
                     /translation="YPLSPVNSMDPLGLYEFKSKNIDDIGIFALAMCNGESINENKEY
                     GGLICKKQGEYLPMNPISSNDNDSVDLRNIKCPEGSERVGDYHTHGFYSDDKGNKVTK
                     ENDVYDSLN"
BASE COUNT          132 a           40 c           70 g           95 t
ORIGIN      
        1 catatccgct gagcccggtg aatagcatgg atccattagg attatatgaa tttaaatcaa
       61 aaaatataga tgatattgga atatttgcat tggcaatgtg taatggagaa tcaattaacg
      121 agaataaaga atatggtgga ctaatatgta agaagcaagg tgaatattta cccatgaatc
      181 cgataagttc aaatgataat gatagtgtag acttgcgaaa tataaaatgc cctgaaggtt
      241 cagagagagt aggcgattat cacactcacg gtttttactc tgacgataaa ggaaataaag
      301 taacaaaaga aaatgatgtt tatgatagtc taaattt
//
LOCUS       DAZGVQ010000508          330 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000508 DAZGVQ010000000
VERSION     DAZGVQ010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.513"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            22..>330
                     /locus_tag="ACHLAG_005567"
     CDS             22..>330
                     /locus_tag="ACHLAG_005567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFP0658586.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMLS
                     F"
BASE COUNT          107 a           65 c           50 g          108 t
ORIGIN      
        1 atataaccaa ggcaaatgca aatgaataac atattacttc cgccaattaa tattccctgt
       61 acacttttcg aaactattag tttattcgat gatttttctg ctgatgatat gcaatatggt
      121 gatatggtag aacaagactt tctctcactc ggcctaagcg atatctcagc aaaagttgat
      181 ccttacagac ttattaaata ccatttccct ggcccaggtt ctattaatgt tgcttttagt
      241 gcctcaagtt ccggaacaaa aatatcacaa cgtgaatgca ctgatatttt atttgcagaa
      301 atgaaagaac tagcaaaaat gctttccttt
//
LOCUS       DAZGVQ010000509          328 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000509 DAZGVQ010000000
VERSION     DAZGVQ010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.514"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>328)
                     /locus_tag="ACHLAG_005568"
     CDS             complement(<1..>328)
                     /locus_tag="ACHLAG_005568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFP0658587.1"
                     /translation="IDLSDLDLTSCHFKGDVISKVSFLSSNLQHVTFECKEIGDSNFT
                     TAIVDNVIFRCRRLHNVIFIKASGECVDFSKNILDTVDFSQSQLTQSNFRECQIRNSN
                     FDNCYLY"
BASE COUNT          118 a           59 c           49 g          102 t
ORIGIN      
        1 cgtaaagata acaattatcg aagtttgaat ttctaatctg acattcgcga aaattactct
       61 gagtaagttg actctgcgag aagtcaactg tatcaagaat atttttgctg aaatcgacac
      121 attcaccact cgctttgata aaaatcacat tgtgtaaacg tcgacatcta aatatgacat
      181 tatcaactat tgcagtagta aaattggaat ccccaatttc tttacattcg aatgttacat
      241 gttgtagatt tgatgataaa aaagacacct tcgatataac gtcaccttta aaatgacaac
      301 ttgttaaatc caaatcagac aggtcgat
//
LOCUS       DAZGVQ010000510          327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000510 DAZGVQ010000000
VERSION     DAZGVQ010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.515"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>327
                     /locus_tag="ACHLAG_005569"
     CDS             <1..>327
                     /locus_tag="ACHLAG_005569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP0658588.1"
                     /translation="GFTAGAEGAFNEASGATAGSSLWGADKPLYHDLVSRTRAALKKN
                     PKNVLLSVIWMQGEKDVSSGRHAEHNALFLAMVNQFRTELADVAEQCTGGTTASVPWI
                     CGDTTY"
BASE COUNT           77 a           76 c          110 g           64 t
ORIGIN      
        1 cgggatttac tgcgggggcc gagggggcgt ttaacgaagc gtcgggagcg accgcaggct
       61 cgtccctgtg gggggcggat aaaccgctgt atcatgacct ggtcagcaga acccgtgcgg
      121 ccctgaagaa aaatccgaag aacgtgctgt tatcagtgat ctggatgcag ggagaaaaag
      181 atgtcagttc ggggagacat gcagaacaca atgcgctttt ccttgccatg gtaaatcagt
      241 tccgtacgga actggcagat gtggcagagc agtgtactgg cgggacaacg gccagcgtcc
      301 cgtggatttg cggtgatacc acgtact
//
LOCUS       DAZGVQ010000511          319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000511 DAZGVQ010000000
VERSION     DAZGVQ010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.516"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            189..>319
                     /locus_tag="ACHLAG_005570"
     CDS             189..>319
                     /locus_tag="ACHLAG_005570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658589.1"
                     /translation="MEKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGIAV
                     "
BASE COUNT           94 a           56 c           94 g           75 t
ORIGIN      
        1 gcgcgctggc gtgctgggga atcgacagat aagaagaata ttttgctgaa aaatgacgtt
       61 ggccaacgcg ggcggataac acgaaatcct gcgaactggc aaaacctaag tgaataaaag
      121 taaaaacccc gtttgttggc agcaagcggg gttttgtgtt tctgaccttg gataaggcaa
      181 gggagaacat ggaaaagtat aaacgaattc tgttgaggtt gactatgaaa aacggccttg
      241 aactgaaagc gcctgtaact gatgacatca gcagagcgct ggcttttgct attaagtggg
      301 tggcggtcgg tattgctgt
//
LOCUS       DAZGVQ010000512          316 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000512 DAZGVQ010000000
VERSION     DAZGVQ010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.517"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>316
                     /locus_tag="ACHLAG_005571"
     CDS             <1..>316
                     /locus_tag="ACHLAG_005571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658590.1"
                     /translation="DFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQPIN
                     SIYSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETISSTP
                     LTQ"
BASE COUNT          123 a           73 c           46 g           74 t
ORIGIN      
        1 gactttaccc caggtataat aaatacatat cacggcgata tttataactg cacaacaaat
       61 accgataatg ttaaaacgcc agacacaccg aagtggcctt gtgataattg ggaagaacaa
      121 caacccatta attccatata ctctggagaa ggatacaacc ctgaacaatt tgatttagcg
      181 caacaacaac tccaacaaat taatgcatgc cacacgaaca ccacatatac aaatgcagac
      241 tactcaaaag ttgtggttca acttgtaagt cttattaata acattgaaac gataagttcg
      301 acaccactta cgcaac
//
LOCUS       DAZGVQ010000513          308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000513 DAZGVQ010000000
VERSION     DAZGVQ010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.518"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            20..229
                     /gene="fumD"
                     /locus_tag="ACHLAG_005572"
     CDS             20..229
                     /gene="fumD"
                     /locus_tag="ACHLAG_005572"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFP0658591.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT          101 a           55 c           80 g           72 t
ORIGIN      
        1 tgataaatag gtataaagaa tgggaaatcg gacaaaagag gatgaactgt accgagaaat
       61 gtgcagagtt gtcggtaaag ttgtgctgga aatgcgcgat ctggggcagg aaccaaagca
      121 tattgttatc gcaggcgtat tacgtacagc attagccaat aaacgcattc agcgcagtga
      181 gctggaaaag caggcgatgg aaaccgtcat taacgcactg gtaaaatgac cattcaggat
      241 gaggcggtaa gcttcatcct gatattcttg cttcagttgc ggtccagtta atcattctaa
      301 ataacaac
//
LOCUS       DAZGVQ010000514          305 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000514 DAZGVQ010000000
VERSION     DAZGVQ010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.519"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT           91 a           57 c           46 g          111 t
ORIGIN      
        1 acctgataac ataacgttgt aaaaaccgaa tgcccagcct ttaaaaaaac agctgggcat
       61 tcggttgctt attaatcgca ataatatatt ggctgtctat tcatcgtgtt gataagatat
      121 atgttatatg aatgtttatt aaatgtagcg attgccttta atgtcacgaa aagggattat
      181 ttgcttgcta ttaatttcac tgcgttctta aacacgtttt tgtattataa atccagtcat
      241 cctgcccgta gaacatcaca cattatcatc ctgttctccc gcttcgttta aatttattta
      301 tcaat
//
LOCUS       DAZGVQ010000515          302 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000515 DAZGVQ010000000
VERSION     DAZGVQ010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.520"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..242)
                     /locus_tag="ACHLAG_005573"
     CDS             complement(<1..242)
                     /locus_tag="ACHLAG_005573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFP0658592.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT           86 a           62 c           69 g           85 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 ag
//
LOCUS       DAZGVQ010000516          297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000516 DAZGVQ010000000
VERSION     DAZGVQ010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.521"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT           73 a           91 c           84 g           49 t
ORIGIN      
        1 cgacgggcga ggagctacac agtggcgcag cactttccgt acacagaggt gcacgcgttc
       61 gcctgcctga cgggcggatt gtgcactggg atgaaaaatc acgaagcctg gtggcagcag
      121 atcaaccgga ggttaaccca tgacacgatt ccattgcgta ctccagcgcc atgatttcag
      181 caccatcatg aacgcacgca ttccggcact gacactgagc ggcgtcagtg ttcgaccgca
      241 gccaggcgac gaactggaaa tcacgatcgc cagtcccgat cgcagcacag acaccgt
//
LOCUS       DAZGVQ010000517          297 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000517 DAZGVQ010000000
VERSION     DAZGVQ010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..297
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.522"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT           71 a           88 c           88 g           50 t
ORIGIN      
        1 cgacgggcga ggagctacac agtggcgcag cactttccgt acacagaggt gcacgcgttc
       61 gcctgcctga cgggcggatt gtgcactggg atgagaaatc acgaagcctg gtggcagcag
      121 atcaaccgga ggtgaaccca tgacacgatt ccattgtgta ctccagcgcc atgatttcag
      181 cgccatcatg agtggacgca ttcccgcact gacgctgagc ggcgtcagtg ttcgaccaca
      241 gccaggcgac gaactggaga tcacgatcgc aaaccccgat cgcagcacag ataccgt
//
LOCUS       DAZGVQ010000518          295 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000518 DAZGVQ010000000
VERSION     DAZGVQ010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.denovo.523"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>295)
                     /locus_tag="ACHLAG_005574"
     CDS             complement(<1..>295)
                     /locus_tag="ACHLAG_005574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658593.1"
                     /translation="GGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRQFSAASGNR
                     KPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMTKA"
BASE COUNT           61 a           81 c           78 g           75 t
ORIGIN      
        1 cggctttggt catgacctcc tcaagggagg ctgccagcgc tgcacgcttg ttttcaaaac
       61 ttttaatctg ttcgccaata ttcattatgg tcttttcctt atgaaaaacg gttgttgact
      121 gtgccgcagc gccggcagaa gatgcgattt tcaccaccgg tttccggttg ccggacgcgg
      181 cagaaaactg gcggtcgtaa gatttaatgg tccggatggt gcattccgca ttcgcgggca
      241 cggtgacggc agacacctcc atcagttccc agcgcagaaa atgcagtccg cctcc
//
LOCUS       DAZGVQ010000519        22808 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.guided.078, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000519 DAZGVQ010000000
VERSION     DAZGVQ010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.guided.078"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..461
                     /locus_tag="ACHLAG_005575"
     CDS             <1..461
                     /locus_tag="ACHLAG_005575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP0658594.1"
                     /translation="CIIFLNQVRTKIGVMFGDPTTSPGGDSPKFYASVRIRLGASVMK
                     DGKEKIGQDVGAECIKNKVAPPYGKCTWKFYFDPTRGLDVIESLVEYMLEEGYLPKNA
                     SGRVEIGDKKYTKSQIVEMYREKPLAEIIAALQAIDDRRAKDNPTESVEE"
     gene            555..755
                     /locus_tag="ACHLAG_005576"
     CDS             555..755
                     /locus_tag="ACHLAG_005576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658595.1"
                     /translation="MIKIYLLAVATGVSVALIYGLLVPSLISTKSDLAVMFGVIVGFG
                     APVIGLIAGRKFINSLIKAKGK"
     gene            759..1589
                     /locus_tag="ACHLAG_005577"
     CDS             759..1589
                     /locus_tag="ACHLAG_005577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP0658596.1"
                     /translation="MKKGLLAVALAAICTMGLTGCDRVEPGYVGIKVNKLGEDKGIGE
                     VVGVGRQWTGLNTELYVFPTFKQMKTYDEPFTFQMSDGTAIGHKIGVAYLVNRDKVTT
                     VFQTYRKGVDDITESDLRQKIADSLNRLASRMTTDSFIDGGKAQLLDNALKDIQKEMS
                     PVGIEVLSLSWVGKPDYPKTVIESINAKVTANQRTLQRQQEVEQRKAEANMLREQANG
                     EADAIRARAQAEADAIRLRGEALRQNPNVMELEAINKWNGQLPQYMTQGANTPFITVK
                     "
     gene            1681..2106
                     /locus_tag="ACHLAG_005578"
     CDS             1681..2106
                     /locus_tag="ACHLAG_005578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658597.1"
                     /translation="MTAIKKLYDAANVALDVIDDEAAKGFPEPDWAHQLRNAIAEMTP
                     PDPTPEETDWQRFIRMYAQEIGPTPTAEQAMLLKYFKEAGEDLPIDDSAYWFHCAWRK
                     YDVIFTQGMGSKDMVVWHLLHIDTAVDRVIEQFFPKQED"
     gene            2162..2437
                     /locus_tag="ACHLAG_005579"
     CDS             2162..2437
                     /locus_tag="ACHLAG_005579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658598.1"
                     /translation="MRVLVRIVTSTVYDVFPLFMVKVDGLNDEETDALIQRTLVEYTG
                     HDADSVMVDDDGVCWHNGNCWYVEETQQISNEDAEHLERILSISTFE"
     gene            2653..2988
                     /locus_tag="ACHLAG_005580"
     CDS             2653..2988
                     /locus_tag="ACHLAG_005580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658599.1"
                     /translation="MKAIPFALLFLSSIVVADTTVYQCEMSVADVKNGALTDVIKAPY
                     GAMVVDSGDQFYVVRDDRVLSSPYLTNRNGKLTGVGEDHFVYNKYKGFYGVHASQQSY
                     LFDDCKEVG"
     gene            2988..3200
                     /locus_tag="ACHLAG_005581"
     CDS             2988..3200
                     /locus_tag="ACHLAG_005581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658600.1"
                     /translation="MALTLAGLEIEKTSGYWRAKGFKQPGILERLEREDGYIVHQRRE
                     WRMYDPETGKLTTKAGTLWGLLKKIH"
     gene            complement(3689..4744)
                     /gene="repA"
                     /locus_tag="ACHLAG_005582"
     CDS             complement(3689..4744)
                     /gene="repA"
                     /locus_tag="ACHLAG_005582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HFP0658601.1"
                     /translation="MSENGNKNIAIVEAFSETDKKTGEVVTLVPNTNNTVQPVALMRL
                     GLFVPTLKSTSRGRKGQMVSMDATAELKQLSLAKAEGYEDIRISGLRLDMDNDFKTWV
                     GIIHAFAKHKVVGDTVTLPFVEFVRLCGIPTARSSAKLRKRLDSSLSRIATNTISFRS
                     KGSDEFYVTHLVQTAKYSVKHDTVELKADPKIFELYQFDKKVLLQLRAINELSRKESA
                     QALYTFIESLPPDPAPISLARLRARLNLTSRTITQNATVRKAMEQLREIGYLDYTEVK
                     RGNSVYFVIHYRRPKLRQAQISTKIDNDETEYSLPDENQDDIIDVVPDEKEGKMVMLS
                     KEELALLEELRKAKTRK"
     gene            5488..6132
                     /locus_tag="ACHLAG_005583"
     CDS             5488..6132
                     /locus_tag="ACHLAG_005583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658602.1"
                     /translation="MDLKRTRWVRRLEDGSYTIESNSNLNKQKLLCDICGIAAKCPIY
                     ETRIKLDKAGVNFHLNSCIRYVPLLAFRKPIIGLDAPYFNTLRSGVTWRDRLSPDKLI
                     CLVSADTGKIIRFGKVDKVYSGPVDEMLRKHSRFNHLCMGGEKIEKVEEVIRKSYGHF
                     LTKDSLLTAIYIRHVKREFDLEYHSEEELNLVDPRPKAGVISINAARKKPTDAL"
     gene            6387..7472
                     /locus_tag="ACHLAG_005584"
     CDS             6387..7472
                     /locus_tag="ACHLAG_005584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HFP0658603.1"
                     /translation="MTLPYGVISDCHYHKWDAFSTTNAEGLNSRLEIQLEATKEAAIA
                     MKKAGCKYMLVAGDTFHVRGTVSPSVLHYVTETYKWIINELDLTVVMLAGNHDLETND
                     SVYSANAAASLSSIGVVIVCGKRPHSIKIGDVTVHLISWRNNHAELISDLKALRKSVE
                     GDNHDVVIHTSINKAIPTMPDVGIDAQELKDIGFRLVLSGHYHNHKEVIPGVISVGAL
                     THQNWGDVGSLAGYMIVNPDGSFSHYETSAPKFINLEDDVADDQIRGNYVRFRAVIEN
                     DEEGIKYQNILKTMGAKGVVCNFIRKSSMMEGTASTTETSKIDSLGESVSAYCKIVHD
                     TDGGFDLSKLDILCQEILTEAESSEAV"
     gene            7702..9618
                     /locus_tag="ACHLAG_005585"
     CDS             7702..9618
                     /locus_tag="ACHLAG_005585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016051677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0658604.1"
                     /translation="MKFLKLQVENFMAIASAEVELDQRGLVLIQGVNSDDSSASSNGS
                     GKSTLMNSLMWCLYGETAHGVKGDDVLSTDHEKNCRVAVTIEDEGKRYAIIRHRKHKE
                     FKNRLIVRGEDGDMTKGKDALTQEFVERLIGASKEVFMASIYASQEAMPDLPGMSDKN
                     LKTIVEEAAGVDRLTRAYAIARERANAAAARMDVVKTKLESTISTIEATQSEIESAKA
                     SSESWEQERSKRYDDALAGLASAEVELTEVELEIRTLPEQIRDTEKAIENERKKLASK
                     EEHDAKLLKVRGAITDIRASIKATENSQADAMNRARNFKTKAEEVGTKVGSPCPTCGK
                     AYCEEDLSTVKESFIEQARQEIGQAKTLAEAMAKHKTNLEKALSIESALVKTTPDVTA
                     IIARIEELTKQLSSLRHREKEVVAIESLVTRARTEVDRISKEINPFIALIARHEDNLV
                     SSKSTFKSLKDELKAIQEQTLLLEKARQVYSPAGVRSHILTSVTPFLNTRTAEYLNTL
                     SDGNITAEWSTMDVTKKGEYRDKFNISVQKKGSSKSFQTLSGGEKRKVRIACSLALQD
                     LVSNRASKNIDLFIGDEIDDALDTAGLERLMGILESKARERGTVLIISHKEMKSWFRE
                     TITLEVKEGRSYVV"
     gene            9608..10351
                     /locus_tag="ACHLAG_005586"
     CDS             9608..10351
                     /locus_tag="ACHLAG_005586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658605.1"
                     /translation="MSFKLSRSQFLQVFAVMQSIKLINGHTSNGAAPRILWGSNNIDG
                     VQFAALLGLISETPLMQSLKSLPPGCIAPILINPFVEGGYLPNVGPGFIASHETEDLN
                     INSEGFFGGMGAHHCMAFTNLIRLANKRVDSLASPGDAFTGFLIQRRDKKYSADKLQF
                     VGKYGEMVEIELQLPHVLANDSADSRRLLGIMRHFIASGVKHAVDKRVTQENEYSDFA
                     NYPQPTLQTAIVANSLEARLLENPIWGTW"
     gene            10361..10930
                     /locus_tag="ACHLAG_005587"
     CDS             10361..10930
                     /locus_tag="ACHLAG_005587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658606.1"
                     /translation="MSKKISVVGVDPSMSNFGLAVGTLDLETDELEIHGLTLVETKAG
                     SNKKTVRVNSDDLRRASEIWRVAKPIIDKANMVFCELPVGSQNSRSQTSYGICIGVLA
                     CVDKPLIQVTPNEIKHFVGNKLTTSKEEIIQWATKKHPKAPWLRRKQSGQDVLVNKNE
                     HLADAVAAIHTGMQTDQFRQVRDVLKSLI"
     gene            11004..13319
                     /gene="nrdA"
                     /locus_tag="ACHLAG_005588"
     CDS             11004..13319
                     /gene="nrdA"
                     /locus_tag="ACHLAG_005588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP0658607.1"
                     /translation="MISIVKRNGQTEPLSEEKYNRVVMYGVEGIRGVSASAVAMGAAA
                     SIFDGITTSQLHEALVKSAADLISPEAPNYSQVAARLNIFKIRKDAFGRYDYPNFYQH
                     IVKNVNKGVYDKDLLTHYSFEEIEELGNYIKPKRDDLFGYAATVQLQSKYLVQNRVTG
                     EIHEGPQHIYMLVGMCLFQNWEDDCAGKTRMEMVKGFYDVTSTFKLSLPTPIMAGVRT
                     PTRQFSSCVLIESGDSLKGISAASAAIIDYVSRRAGIGIGFGRIRALGSEIRNGEATH
                     TGVIPFLKHFQTAVKSCSQGGVRGGAATAFYPIWHLEVESLLVVKNNRGIDENRVRHL
                     DYGVMSNRLMYRRLVRSENITLFSPHDVPDMYEAFFTDQDLFEKLYHKYEADDSIRKK
                     SVPAIELFSSLMQERASTGRIYIANVDHINEHGAFIPALAPVRQSNLCMEITLPTRPL
                     AFTDDPNGEIALCTLSAFNLGAIRSLESLKEVAFYAVAALDSLLDYQDYPMEAAEVPA
                     KARRSLGIGVTNFAYYLAKNGVRYSDTAGNKLVHETFEAIQYYLLDASCRLAEAKGEC
                     DWFEQTKYAIGQLPIDHYRSSLDESGETNFELKMPWEELRERIAKYGLRNSTLTAQMP
                     CETSSQITNSTNGIEPPRGPVSVKSSKDGIVKMVVPEFEKLKEQYEYLWDMPDNRGYL
                     TKVAIIQKFFDQAISANTNYDPSRFEGDKVPMMTLLSDLLLAYKMGVKTLYYHNTRDG
                     AGKRDDDEPQNPLTQAVAVEPEDECDGACKI"
     gene            13426..14568
                     /gene="nrdB"
                     /locus_tag="ACHLAG_005589"
     CDS             13426..14568
                     /gene="nrdB"
                     /locus_tag="ACHLAG_005589"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP0658608.1"
                     /translation="MSYSTFRLGANDATKEPMFLGQSVNVARYDQQKYRDFEKLIERQ
                     LSFFWRPEEVDISSDRIDFNTKLRDHERHIFLSNLRYQTLLDSVQGRSPNATLLPLIS
                     IPELETWVETWSFSETIHSRSYTHIIRGMVDDPSIVFDGIVTDEEIINRAISISAEYD
                     RLYGMTCERQSLGEKEFERLYVNEYGWEPYPLHRQLFRTLVSINALEAIRFYVSFACT
                     FAFGERKLLEGNTKIMRFIARDEALHCEGTERMIRFMRTGREGLLWKEIAADEENVIY
                     DTMKSVAEQEMNWADYLFKDGSMIGLNADILKTYVKYRTNLAMNRLGLKALFPEVTTD
                     PLVWMNKWLLTDTLQIAPQEAEQSTYLVGQIDSTVDKASLSQFADL"
     gene            14646..15515
                     /locus_tag="ACHLAG_005590"
     CDS             14646..15515
                     /locus_tag="ACHLAG_005590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP pyrophosphohydrolase"
                     /protein_id="HFP0658609.1"
                     /translation="MNFTKLTDHLKLATDRLIGFKPEPYELHEGHGVATESIYKMVDQ
                     FHELFQHPRRVMPTPELLRLRASLIHEEAVVEGIPAAMNGDIEQLLDAMADFLYVGVG
                     TMVAIKGGISTGMTYYTQEQSIDRFMQTIFVPGNTVFDDMAMPFQEAREASCMLEELA
                     DKLENKTVKDSELIQELRRVMNKIYVACMMTYRLADFLGINVVELVGEIHRSNMTKLW
                     PADVEERRQAVANCKYDSSDLGFRHADGTDKMIGFRISDGKILKSPTYSDVDLSSFVE
                     QAKASAMYGMIKK"
     gene            15686..16789
                     /locus_tag="ACHLAG_005591"
     CDS             15686..16789
                     /locus_tag="ACHLAG_005591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP0658610.1"
                     /translation="MSLLLNREHTNGQVTNASYAKVIETVLKSGVQADDRTGTGTLST
                     CYVPSYYMLTGGTVPLISGKAVNLKPLLVELEWYLKGTGNIQFLKDNGVKIWDAWADE
                     NGDLGPVYGKQWRRWEDTRIVSHSEYLSKIATFRERGYKVEGYLGISEDRVVLSREID
                     QLQRIVDTLRTNPTDRRIMLNAWNVGELEDMKLPPCHFVFSLWSRELDFETRLTMATD
                     IGLQHSRLGYESIYTKMLYDLEMDGSVTEAELDELGIPKRILNSCLVQRSVDTFVGMP
                     FNIAGYGILTHFLAKITGHMAGAFVHFGFDVHLYNNHMEGVCELMKRQAPEHSDPVVI
                     FPHEWSELDDFKWDEVLILGYDPLPWIKVPVAV"
     gene            16791..17204
                     /locus_tag="ACHLAG_005592"
     CDS             16791..17204
                     /locus_tag="ACHLAG_005592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658611.1"
                     /translation="MARGMYVLCEIEGVLANASHRKSVSDADAGQLIAGDELIFPTSR
                     MLRGFARSGAEVVLISSRSETLEAPTKRWLKDFGVDYDWLHLVPNGTSYEKHIKRTLA
                     EHKGDLLIAALVHDPRLRAALADSHHRPVIYEVSK"
     gene            17201..17677
                     /locus_tag="ACHLAG_005593"
     CDS             17201..17677
                     /locus_tag="ACHLAG_005593"
                     /inference="COORDINATES: protein motif:HMM:NF047324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFP0658612.1"
                     /translation="MKMIAAVGRNYEIGIANELPWRCSTDLKLFKRLTKNATVVMGRK
                     TMESLKRPLPERHNLVLTRSHGFVPNGFYPAGVDDVLRLPEPVWVIGGEQIYSLFMPH
                     VEEIWLSHIGVDVPNADAFFPASMMRNLGFVPVETAYTQRASEEEPGFSQIVYRRS"
     gene            17677..18321
                     /locus_tag="ACHLAG_005594"
     CDS             17677..18321
                     /locus_tag="ACHLAG_005594"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP0658613.1"
                     /translation="MDYRIGITGAQGSGKTTLAKYIDKHYGIPYVDAGVGSLMSRLGV
                     RVGESMPLYERLQIQMEIAKHIELLTRGVEGFVIDRTPADVMAYTLDLVGHTNEDRCI
                     ELALDIEKFCHKTAISNFNAIAGLRPGVALSERDYLRSQRASLDRLYVARIDALMCGE
                     LTKIHLHPQRGDLQTFVVSNRYRTVEARARSVMRMLDNAVEKIENRFCGRVTVH"
     gene            18383..18802
                     /locus_tag="ACHLAG_005595"
     CDS             18383..18802
                     /locus_tag="ACHLAG_005595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658614.1"
                     /translation="MFSEMLLEDELDRKTTEALIRVADEHSRSLMSDREARLAIRAIF
                     ETAQGLVGAQVGEAINIAMSQFSEDSKKPLFPMHLMLAGGTVLYISVCLDSNQINILN
                     TASGKWKDPIVCETSEETLKKAAQFVRSALLKGAKKL"
     gene            18812..19369
                     /locus_tag="ACHLAG_005596"
     CDS             18812..19369
                     /locus_tag="ACHLAG_005596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HFP0658615.1"
                     /translation="MTTIVAGIDIESTGLDFLAGHKIIEIAITRYELETQRHIDSLEM
                     RFNPRRNIDPKAQAVHGISLEQLAAEPLLSNHASEIGAYMGACSAWIAHNGEAFDIPF
                     IRHEFSGYGVRLPDVPVIDTMLSGLWATEDGKRPRLEELAFSLGFIYDHAKAHSALYD
                     TNLMMQCFFKARNKYGFFKLPSEIV"
     gene            19530..20339
                     /locus_tag="ACHLAG_005597"
     CDS             19530..20339
                     /locus_tag="ACHLAG_005597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658616.1"
                     /translation="MSSVENVMTNDDLDELTAMLQSLDEPVKKAAQVENTDDIDDLLL
                     GLDAGVAMSSDDVAEELFNEEKAGDFSSALNELELAHEPINVINAESVESAENEPEQL
                     GLIEVEECVDVNDELKVQQSNDSNTNKKARTARGPRFTLSDKDDSFFNKAGLETDIFL
                     DAYENAPVKAKDKILNLLNWFSGGPDISVYTVISMRHLLTEKKATSNSIKIALMSNPE
                     KPYPLNTASTQAGQMMAVFPATGIAVRDGGNLTLNEESPIVKKFVAEYTIG"
     gene            20523..21116
                     /locus_tag="ACHLAG_005598"
     CDS             20523..21116
                     /locus_tag="ACHLAG_005598"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFP0658617.1"
                     /translation="MIAAEKIKQRKRDNSLRDLWRTPDWLFSAIQRYLGVTFDVDVAC
                     NKDNAKLPNFIGVERDALKSEWGQPGTIAFLNPPYSKISPWIDAAIREQARGVTTVML
                     IPQSLDTKWYERATECANETIILSGGRVAFVEPDVNLGQVEVNINPGGSMLVVFRGFC
                     QDAGHSISKIPLDVMKSLGGYDPANVIRKKRQSKKAA"
     gene            21303..21533
                     /locus_tag="ACHLAG_005599"
     CDS             21303..21533
                     /locus_tag="ACHLAG_005599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658618.1"
                     /translation="MSYPTNVVALVESDFLAQAREMMKDREQAFNLYEWAIKCLHLGE
                     HRELVEQLLGELINEVFALNVQLHGRKNNQSK"
     gene            22050..22710
                     /gene="rnhA"
                     /locus_tag="ACHLAG_005600"
                     /pseudo
     CDS             22050..22710
                     /gene="rnhA"
                     /locus_tag="ACHLAG_005600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
BASE COUNT         6335 a         4915 c         5512 g         6046 t
ORIGIN      
        1 cctgcatcat cttcttgaat caggttcgca caaaaatcgg tgtaatgttt ggcgacccta
       61 ctacgtcgcc aggtggagat tcaccgaagt tctacgcttc tgtacgtatc cgtcttggtg
      121 catcggtgat gaaggatggt aaagagaaga tcggccagga cgtaggcgca gaatgcatca
      181 aaaacaaagt tgcaccaccg tatggcaaat gcacctggaa attctacttc gatcctactc
      241 gtggcctcga cgttatcgaa tcgctcgtcg agtacatgct ggaagaagga tacctgccaa
      301 agaacgccag cgggcgagtt gaaattggtg acaagaaata caccaaatcg cagatcgtcg
      361 agatgtatcg ggagaagcca ctggctgaaa tcattgcggc tttgcaggca atcgacgacc
      421 gaagagcaaa agacaacccc accgagtcag tagaagagta aacacaaggc gtccacagga
      481 cgcctttttt tatctcttga aaatatataa gtacttactt attattttcg cataacaacc
      541 acataggaaa acacatgatc aaaatctatc tattggcagt agccacaggc gtttcagtgg
      601 ctctcatcta cggtttactg gttccgtcgc tgatttctac caagagtgat ttagccgtca
      661 tgtttggagt tatcgttggt tttggtgctc ctgtaatcgg tcttattgct ggtcgtaagt
      721 ttatcaactc attaatcaaa gcaaagggga aataagtaat gaagaaaggt ttacttgcag
      781 ttgctctggc ggctatttgc acaatgggtc ttactggctg tgatcgcgtg gagcctggat
      841 acgttggcat caaagtaaac aaattaggtg aagacaaagg gatcggtgaa gtggttggcg
      901 ttggtcgcca atggacaggt cttaacaccg aactttacgt attcccgacc ttcaaacaaa
      961 tgaagaccta cgacgagccg ttcacattcc agatgagtga cggtactgct attggtcaca
     1021 aaattggcgt tgcgtatctg gttaatcgtg acaaggtaac gacggtgttc cagacctatc
     1081 gcaaaggcgt agacgatatc accgaatcag atctgcgtca gaaaattgcc gactctctaa
     1141 accgtttggc cagccgtatg accactgact catttatcga cggtggtaaa gcgcaattgc
     1201 tggacaacgc actgaaagat attcagaaag agatgtctcc ggttggtatt gaggtactga
     1261 gcctgtcatg ggttggaaag cctgattacc caaaaaccgt cattgaatct atcaacgcca
     1321 aagtaacggc taaccagcgt actctgcaac gtcagcagga agttgaacaa cgtaaagctg
     1381 aggcgaatat gctacgtgaa caggctaatg gtgaagctga tgctatccgt gctcgtgcgc
     1441 aagcagaagc agacgccatt cgtctgcgcg gtgaagctct gcgtcaaaac ccgaacgtta
     1501 tggaactgga agccatcaat aaatggaatg gccagttacc gcagtacatg actcaagggg
     1561 ctaacactcc tttcattaca gtgaaataac tcccctaaaa agttcaggcg tccagttgga
     1621 cgcctttttt atcgcaatta tcttattaag aaaataattt gtttaaaagg ataagaaaac
     1681 atgacagcta ttaagaaact ctacgatgcc gcaaacgtgg ctctggatgt tattgatgat
     1741 gaagcagcaa aaggctttcc tgaacctgat tgggcgcatc agctacgaaa cgctatcgca
     1801 gaaatgaccc caccagatcc aacccccgag gagacagact ggcagcgatt catccgtatg
     1861 tacgctcagg aaataggtcc aacgccaacg gcagagcagg caatgctgct gaaatacttc
     1921 aaagaggcgg gagaggattt accaattgat gactcagcat attggttcca ctgcgcatgg
     1981 cgtaagtatg acgtgatatt cacacaaggc atgggaagca aagatatggt tgtgtggcat
     2041 ctactccata tagacacagc cgttgacaga gttattgaac agtttttccc taaacaagaa
     2101 gattgatcgc ctattcataa ctaacaaaat aagtaaacac taaccacaaa aggaaaaaca
     2161 catgagagtt ttagttcgaa tcgttaccag cactgtctat gacgtgtttc cgctttttat
     2221 ggtcaaagtc gatggcctta acgatgaaga aactgacgcg ctgatccagc gtactcttgt
     2281 tgaatataca ggtcatgacg ctgattcagt gatggttgat gacgatggtg tttgttggca
     2341 taacggcaac tgttggtacg tagaagagac tcaacaaatc agtaatgaag atgctgaaca
     2401 tcttgagcgt attttaagca tcagcacttt tgagtgagtt tacagtaaag tttatataag
     2461 ttagtatcta cctatcatga agatttttat tgaatacttg ttactcatcg tttcaatagc
     2521 ttttgtcatc gactgcattt tcaccggtgt cattcgtaaa gtcttttccc cggtgcacga
     2581 cgtagtcata aacgctttgg ctatcgtgct cgtatttaat tcagcatttg atgtaatcaa
     2641 agaggtggca gcatgaaggc catcccattc gctctgttgt tcctttcctc gatcgttgtg
     2701 gccgacacca ctgtttatca gtgtgaaatg tctgtagccg acgttaagaa tggcgctctt
     2761 accgacgtca taaaagcacc atatggagcg atggtcgtag acagcggcga ccagttctat
     2821 gttgtgcgtg acgatcgagt gttgtcatcc ccatatctca caaatcgtaa tggcaaatta
     2881 accggcgtcg gagaagacca cttcgtatac aacaaataca agggcttcta tggcgttcac
     2941 gcttctcagc aaagctacct tttcgatgac tgcaaggagg ttggataatg gcattaacac
     3001 tggcaggtct ggaaatcgag aaaacaagcg gctactggcg tgctaagggt ttcaagcagc
     3061 ctggcattct tgagcgtctg gaacgtgaag atgggtatat cgtccaccag cggcgtgaat
     3121 ggcgtatgta cgatccagaa acaggaaaac tgactacaaa agccggaaca ctttggggcc
     3181 tgttgaagaa aatacactaa tagcaccaac cactgcggtg agtagccagc tcaccgcatt
     3241 cgtatcagct cattatccac tgtggtctgc agtcctctct tcctacatat ctgctaacca
     3301 ctgtagcgag tagacagcct ttcattcgca tcaaacaacc actatggtga gtattggagt
     3361 gagatgtcca gcgggtatcc actatggtga gtaagccttt gctgttttca gtggatatcc
     3421 actcgccaca gtggatacgc tcaaagatga gggcgacaac aaccactata gacagtaaat
     3481 tttccaagat acccagtgac cactaacctc gcagcccttg tttcatcagt gttagtgatc
     3541 actaacattt gattcagtat ttgcattcta ctgcctacag tggttatcaa cagataacga
     3601 tactcactat agtggatagc gaattacaga tataaataag gctcactaca gtggatagtg
     3661 agccttacta ctaactacag tggttgggct atttgcgagt ctttgccttg cgcaactctt
     3721 cgagaagcgc cagttcttct ttactcaaca tgaccatttt gccttctttt tcgtcaggca
     3781 caacgtcgat aatgtcatct tgattctcat ctgggaggct atattccgtc tcatcattat
     3841 cgatttttgt tgatatttgt gcctggcgta actttggccg cctatagtgg atgacgaagt
     3901 agaccgagtt tccgcgctta acttccgtgt aatcgagata tcctatctct cgcagctgtt
     3961 ccatagcctt tcttaccgtc gcattctgag taatagtgcg acttgtcaga ttaagtctgg
     4021 cgcgtaaacg ggctagtgat attggtgccg gatcaggcgg caaactttca ataaaagtat
     4081 aaagcgcctg agcggattct tttcttgaga gttcgtttat agcccggagt tgcagaagaa
     4141 cctttttgtc gaactggtag agttcgaaaa tcttgggatc tgctttcagt tcgaccgtat
     4201 cgtgcttgac actatatttt gccgtttgca caagatgcgt tacgtaaaac tcatcagagc
     4261 ctttgctacg gaacgagatg gtatttgtgg caatacggct tagcgaactg tccaggcgtt
     4321 tgcgtaactt cgctgatgac cttgcggtcg gaatgccaca cagccttaca aactcaacga
     4381 acggcaacgt gacagtatcg ccaacgacct tgtgtttggc aaacgcgtgg attattccca
     4441 cccacgtttt aaagtcgtta tccatatcaa gccgaaggcc agagatcctt atgtcttcgt
     4501 acccttcggc ttttgccaga gacagctgtt tgagttcagc agtggcatcc atagagacca
     4561 tttgcccctt tcttcctctg gatgtcgatt tcagcgtcgg aacgaagagg cccagacgca
     4621 tcaaagcaac aggctgaaca gtgttattag tgttaggaac caacgtaaca acttcgcctg
     4681 tctttttatc tgtttctgaa aatgcttcaa cgatcgctat gtttttattg ccgttttcgc
     4741 tcattccaaa tgtctcttgt tattcgacgg ctttggtggc ctttgctgat tacagtggat
     4801 agtagcactc atcacagcgg ttatcctacc gtctatagtg gtttttctcc tctctatagt
     4861 ggttgctttg ctccctatag tggattattc gctctctata gtggatatcg atcatgtctg
     4921 aaggcagatg gcgcaacggt ttgaggtggg cggggatctt attgggtctt tatgggtctc
     4981 tttggttctc tttgtgatct gaattactgg atcgggcctg tggataaaaa tcagaggatt
     5041 tcaagaacag gtaactctac tcttctgtgg atatttaagg acataagaat catgactaag
     5101 agtagagttt aaaatatatc tatgtatatc agtatgttaa tgaagaatta agtcattaag
     5161 ttatccataa aagacaatgt gctctactct ctacagtgga tatatacagc tctccagagt
     5221 ggttgtttta ctcttcatag cggttattca gctctctaca gaggtttctt tgctctccat
     5281 agtggatggc aacacccttt cagaccagta accgcaacgg ctagaggcga tcggggatct
     5341 ctttggatct tctatttgat cttacttggg atctcttatt tggatctatc cagtggataa
     5401 ctgggataag taaaacaggc aaatgcagat taaggtatgc ctaatgagtt atcgtagcat
     5461 cggccaataa ctactgaaaa aacgattatg gatctaaaac gcacgcgctg ggttcgtcgt
     5521 cttgaggacg gctcctacac tatcgaatca aattccaacc tgaataagca gaagttactt
     5581 tgtgatatct gcggtatagc ggcgaagtgc ccgatctacg aaaccagaat taaacttgat
     5641 aaggctggtg tgaactttca tttgaatagt tgtatcaggt acgttccatt gctcgcattt
     5701 cgtaaaccga tcatcggatt ggatgcgccc tacttcaaca cactccgttc aggagtaacg
     5761 tggcgagatc gtttatcacc agacaaactg atttgccttg tatccgcaga tacaggaaaa
     5821 atcattcgtt ttgggaaagt agacaaggtt tactcaggcc cagttgacga gatgttgcgg
     5881 aaacacagcc ggtttaatca cctctgcatg ggtggtgaga aaatcgagaa ggttgaagaa
     5941 gtgatccgca aatcctacgg acacttcctg accaaagata gcctgctcac tgcaatctac
     6001 atcagacatg taaaacgtga gttcgacctt gaataccata gcgaagaaga actgaacctt
     6061 gtcgatccac gtcctaaagc tggcgtcata agcataaatg cagcgcgtaa aaagcccact
     6121 gacgcgctgt aaccctccag atcgtatatt ggcgtagata gaatctacgc cccctcaaaa
     6181 tagctctcat agcgttctac agtgatccag tcttattttt agtcatacag acaagcaaag
     6241 ttgcgccacg ataaataggt atatacttac ttataaattt tgtatattaa ggcgctcgtt
     6301 tcattcctaa cataccgtta tgcatagttg tttacctcct cattgctctt agaatttgta
     6361 tcaaaataac cacaaaggaa aaacacatga ctttgccata cggcgtcatt tctgactgcc
     6421 actaccacaa atgggatgcg ttctccacga cgaacgctga ggggcttaac tccagacttg
     6481 aaatacagtt ggaagcaacg aaagaagcag ccatcgccat gaagaaggcc ggttgtaagt
     6541 acatgttggt tgccggtgat acatttcacg tccgaggaac tgtgtcccct tctgttttgc
     6601 attacgtaac tgaaacgtac aagtggatta tcaacgagct tgatctgaca gtagtaatgc
     6661 tggccggtaa tcacgatctt gaaaccaacg attcagtata tagcgccaac gcagcagcat
     6721 cgctgagttc tatcggcgtg gtaatcgtat gtggcaagcg cccacactca ataaaaattg
     6781 gtgatgtgac tgtccacctg attagctggc gtaacaatca tgcggagctt atcagcgatc
     6841 tgaaagcatt acgtaagagc gtagaaggtg ataatcatga cgttgttatc catacatcca
     6901 ttaacaaagc cattccaaca atgcctgacg tcggtatcga tgcgcaggag ttaaaggata
     6961 tcggctttcg tctcgtgctt agtgggcatt accacaacca caaagaggtc attcctggag
     7021 ttatcagtgt cggtgcactg actcatcaaa attggggaga tgttggatct ctggctggct
     7081 acatgatcgt aaacccggac ggcagtttca gtcactacga aaccagtgcg cctaaattca
     7141 ttaacctgga agatgatgtt gctgatgacc aaattcgcgg caactacgtg cgtttccgcg
     7201 ccgtaattga gaacgatgaa gaaggcatta agtaccagaa catcctcaaa acaatgggtg
     7261 caaaaggtgt cgtgtgcaac ttcatccgta agtcatcaat gatggaaggg acagccagca
     7321 caactgaaac cagcaaaatc gatagcctgg gagagtcggt atctgcttat tgcaagattg
     7381 tccacgatac tgacggcgga tttgatctga gcaaattgga tattttgtgt caggaaatcc
     7441 tcaccgaagc ggagagttcg gaggctgtgt gaagcaaagt cgttatggga gctttcgaga
     7501 ctttgccatc acgatgaaaa gacttgaacg aggccagacg gtgatgtttc acaagcccta
     7561 cccgccacaa ggaaatcccg tagcgtttta tcttggaagg ttaacaagaa aaggcgtatt
     7621 gaggcgcaga tccttcccgg cgcatacgga gttcagattg aaagaaggcc aaaagctaac
     7681 acacggtatc agaggtgtta tatgaagttt ttaaagctcc aggttgagaa ttttatggct
     7741 atcgccagcg cggaggtcga gttagatcag cgtggtttag tgctcattca gggtgttaat
     7801 agtgatgata gttccgcatc aagtaatggc tctggaaagt caactctaat gaatagcctg
     7861 atgtggtgtc tttatggcga aacagctcat ggtgtgaagg gtgacgatgt gttgtctacc
     7921 gaccatgaaa agaactgtcg tgttgcagta accatcgagg atgaaggcaa gagatacgca
     7981 atcattcgtc accgtaaaca caaagagttc aaaaatcgtc ttatcgttcg tggtgaagat
     8041 ggcgatatga cgaaaggcaa agatgcgctg acgcaggagt tcgtcgagcg tctgatcggt
     8101 gcatctaaag aggttttcat ggcttccatc tatgcgagcc aagaagctat gccagattta
     8161 cctggaatgt ccgacaaaaa tctcaaaacc atcgtagaag aagccgctgg cgttgacaga
     8221 ctgacacgcg cctacgctat tgctcgtgag cgagctaatg cagctgccgc acgtatggat
     8281 gtggttaaaa ccaaattgga gtcgacaatc tcgaccattg aggcaacaca gtcagaaatt
     8341 gagtcagcga aagcctcctc tgaatcatgg gagcaagagc gttctaaacg ttatgacgat
     8401 gccctggctg ggctggccag tgccgaagtt gagttaacgg aagttgaact tgagatccgc
     8461 actcttcccg aacagatacg tgataccgag aaggcaatcg aaaatgagcg caaaaagtta
     8521 gcctcaaaag aagaacatga cgccaagttg ctcaaagttc gtggtgcgat aactgatatt
     8581 cgggcaagca tcaaagctac agaaaatagt caggctgatg caatgaaccg cgcgcgtaat
     8641 tttaagacca aagcagaaga ggttggtact aaagtgggat caccatgccc tacttgtggc
     8701 aaagcctact gcgaagaaga tctatcaacg gtgaaggaga gtttcattga acaagcacgt
     8761 caggaaattg gtcaggcgaa gacacttgca gaggcaatgg ctaaacacaa aacgaatctt
     8821 gagaaagcgt taagcattga gtctgccctt gttaaaacga cacctgatgt aacggctatc
     8881 attgcccgga ttgaagagct tacgaaacaa ctctcatctt tgcgtcatcg tgagaaggag
     8941 gttgttgcta ttgagtctct tgtgactcgt gctcgtactg aggtcgatcg tatatcaaaa
     9001 gagattaatc cgtttattgc tcttatcgcc agacacgaag ataacctggt atccagtaag
     9061 tctaccttca agtccttaaa agatgagttg aaggctattc aggaacaaac gttgctattg
     9121 gaaaaagctc gtcaggtcta ctctcctgcc ggggtgcgtt ctcatatttt gacgtctgtt
     9181 acgcctttcc tgaatacacg cactgccgag tatctcaata cgttatctga cgggaatatt
     9241 actgctgagt ggtcgacgat ggatgtcact aaaaaaggtg agtatcgcga caaattcaac
     9301 attagtgtgc agaagaaagg ttcaagtaag tcgttccaga ccctctctgg tggtgagaag
     9361 cggaaggttc gcattgcgtg ttctttggca ttgcaggatc tggttagtaa ccgggcgagt
     9421 aaaaacatcg atttgtttat cggcgacgaa attgacgatg cactcgatac agccggtctt
     9481 gaacgcctca tgggtattct ggagtccaaa gctcgcgagc gaggtactgt gctgattatc
     9541 tcccataaag agatgaagtc atggttccgg gaaactatta cgctggaagt taaagagggg
     9601 cgcagctatg tcgtttaaat taagccgctc gcagtttttg caggtatttg cagtgatgca
     9661 gtcgataaaa ctgatcaatg ggcatacttc caatggtgcg gctccacgta ttctgtgggg
     9721 aagcaacaat attgacggag tacaattcgc cgcgttgctt ggtctaatat ccgagacgcc
     9781 attgatgcaa agtttgaaat cactaccacc tggatgtatt gcgccgatcc tgattaatcc
     9841 ttttgttgag gggggatatc ttcccaacgt cgggcctggg tttattgcat cccatgaaac
     9901 tgaagatctt aacattaata gcgaagggtt ctttggggga atgggtgcgc atcactgtat
     9961 ggctttcacg aaccttattc gacttgccaa taagcgggtg gatagtttgg catcgccagg
    10021 tgatgctttt actggtttcc ttatccaaag gagggataaa aagtacagtg cggacaaact
    10081 acagtttgtt ggtaagtatg gagaaatggt agaaatcgaa cttcagctcc ctcatgtttt
    10141 agcaaacgat agtgcagaca gtcggaggct gttgggcatc atgcgtcatt tcatagcaag
    10201 tggcgttaaa catgccgtag ataaacgtgt cacgcaggaa aatgagtatt cagactttgc
    10261 aaactatccc caaccaacgt tgcaaacggc aatagtagcc aattcgttgg aggcgagatt
    10321 attggaaaac cctatatggg gaacatggta aggagactat atgagtaaaa aaatcagcgt
    10381 agttggtgtt gatccctcaa tgagcaactt tgggcttgct gtgggcactt tagaccttga
    10441 gacggacgaa cttgagattc acggccttac tcttgttgag actaaagcgg ggagtaacaa
    10501 aaagaccgtt cgtgtgaaca gtgacgatct gcgccgagcc agtgaaatat ggcgtgttgc
    10561 gaagccaatc attgataagg caaatatggt tttttgtgag ctaccggttg ggagccaaaa
    10621 ctctcgttcg cagacgtctt acggtatttg tatcggtgta cttgcgtgtg tggataagcc
    10681 attgatccag gttactccaa acgaaatcaa gcattttgtc ggcaataaac ttactacatc
    10741 gaaagaagag attatccagt gggctacgaa aaaacaccct aaagcaccgt ggctgcgtcg
    10801 taagcaatct ggacaggatg ttctcgtgaa caaaaacgaa catttggctg atgcggtggc
    10861 tgccatccat accggtatgc aaacagatca gttccgccag gtgcgcgatg ttcttaagtc
    10921 tctcatttga tttcatagat aggtaagtgc ttatctatta acatgggcca ctatatttag
    10981 tggccctctt tatttggtga tacatgataa gcatcgtaaa acgtaacggc caaacagagc
    11041 cgttatccga agaaaaatac aaccgcgtcg taatgtatgg cgtagaaggc attcgtggtg
    11101 taagcgcatc cgctgtagca atgggagctg cggccagcat ttttgatggg attaccacca
    11161 gccagttgca tgaggctttg gttaaatctg ccgctgattt gatctcacca gaagcaccaa
    11221 attactcaca ggtggctgcc cgcctgaaca tttttaaaat ccgcaaagat gccttcggtc
    11281 gttacgacta tccgaacttc taccaacaca ttgtcaagaa cgttaacaag ggcgtttatg
    11341 acaaggattt gctgacacat tattcatttg aagagatcga agaactcggc aattacatta
    11401 agcctaaacg tgacgatctt tttggctatg cagctacggt gcagttgcaa agcaaatacc
    11461 tcgttcaaaa ccgtgttact ggtgagattc acgaaggccc gcaacatatc tatatgctgg
    11521 tgggcatgtg tctgttccag aattgggaag acgactgcgc gggcaaaaca cgtatggaga
    11581 tggtcaaagg tttctatgac gttacaagta cgttcaaact gtctctgccc acaccaatca
    11641 tggctggcgt ccgtactcca acccgtcagt tctccagttg tgtgctgatt gagtctggcg
    11701 atagtctgaa aggtattagt gcagcttcag ccgcaattat cgactacgtt tcacgtcgag
    11761 ctggaattgg tattggtttt ggccgtatcc gtgcgctggg cagtgagatc cgcaatggtg
    11821 aagccaccca taccggagtt attccattcc tgaagcattt ccagactgct gttaaatctt
    11881 gctcgcaagg tggtgtccgt ggtggcgcag caacagcgtt ttacccgatt tggcatcttg
    11941 aagttgaaag tctgctggtg gtgaaaaata accgtggtat tgatgaaaac cgcgttcgcc
    12001 atcttgatta cggcgtcatg agtaaccgtc taatgtaccg tcgactcgtc agaagcgaga
    12061 acatcactct gttcagcccg catgatgtgc ctgatatgta cgaagccttc ttcacagacc
    12121 aggatctgtt tgaaaagctg taccataaat acgaagccga tgattcaatt cgcaagaagt
    12181 cagtacctgc cattgagctg ttctcatctc tgatgcagga acgagcgtcc acgggccgaa
    12241 tttatattgc gaacgtcgat catattaacg agcatggcgc tttcattcct gctcttgcac
    12301 ctgttcgcca gtcaaacctg tgcatggaga tcactctacc cactcgtcca ctggcattta
    12361 ccgacgaccc gaacggtgag atcgcgctat gcactttatc cgcttttaac ctcggagcca
    12421 tccgttcact ggagtctctt aaagaggtgg cgttctatgc cgttgctgca ctggattcgt
    12481 tactggatta ccaagactat ccgatggagg cagccgaagt gcctgccaaa gctcgtcgta
    12541 gcttgggaat tggtgtaacc aactttgctt attacctggc aaagaatggc gttcgttatt
    12601 ctgataccgc tggcaataaa ctggtgcatg aaacgttcga agctatccag tattaccttc
    12661 ttgatgccag ctgccgactt gctgaagcaa aaggtgagtg tgactggttt gagcagacca
    12721 agtacgcaat tggtcagttg ccgatcgacc attaccgttc ttcattagac gaaagcggcg
    12781 aaaccaactt tgagttaaag atgccgtggg aagaactgcg tgaacgtatt gcaaaatacg
    12841 gccttcgcaa ctccacactg acggcacaaa tgccatgcga gacttccagc cagatcacta
    12901 attccaccaa cggcatcgaa ccgcctcgtg gcccggtgtc ggtgaaatct tctaaggacg
    12961 gcatcgttaa gatggtcgtg cctgagtttg aaaaactgaa ggaacagtat gaatacctgt
    13021 gggatatgcc ggacaaccgc ggctatctga caaaggtggc gatcatccag aagttctttg
    13081 accaggctat ttcagccaat accaactatg acccttctcg ctttgaaggc gataaagtcc
    13141 caatgatgac gctactgtca gatttgcttc tcgcctacaa gatgggagtt aaaacgcttt
    13201 actaccacaa caccagagat ggggcaggaa agcgtgatga cgacgaaccg cagaatccac
    13261 tgacgcaagc tgtagccgtc gagccagaag atgagtgcga cggagcctgc aaaatctgac
    13321 atatggtggg ggatatcccc accttctctt tgatttgtaa gccttgttta aacacataag
    13381 ataacaactt gtttaaacgc accaaaaaag aaaaaggaaa aacacatgtc atattcaacg
    13441 ttccgtttgg gtgctaatga tgcaaccaaa gagcctatgt tcctcggaca atctgtcaac
    13501 gtggcacgtt acgatcagca aaaataccgt gattttgaaa agttgattga acgtcaattg
    13561 tctttcttct ggcggccgga agaagttgat atttcgagtg atcgtatcga cttcaacacg
    13621 aagctgcggg accacgaacg tcacattttt ctgagcaatc tccgttatca aacgttactc
    13681 gattcagttc agggacgtag cccaaatgca acgcttctgc cgcttatctc tattcctgaa
    13741 ctggaaacgt gggttgaaac atggtctttc tctgagacta tccatagccg cagctacacc
    13801 cacattattc gtggcatggt ggacgatccg agtattgttt ttgacggtat tgttacggat
    13861 gaagaaatca tcaaccgagc gatcagtatc tctgctgaat atgacaggct ttatgggatg
    13921 acctgcgagc gccagtcgtt aggtgagaaa gagtttgaac gtctgtacgt aaatgaatat
    13981 ggctgggagc catacccttt gcaccgtcag cttttccgca cgttggtgtc cattaatgcg
    14041 cttgaggcga tccgtttcta tgtaagtttt gcatgtacgt ttgcctttgg cgaacggaag
    14101 ttgcttgagg gtaacaccaa aattatgcgc tttattgccc gtgatgaagc tctgcattgc
    14161 gaaggaactg aacgcatgat ccgcttcatg cgtaccggtc gcgaaggctt attgtggaaa
    14221 gagattgctg ctgatgaaga aaacgtcatt tacgacacca tgaaatcagt cgccgaacaa
    14281 gaaatgaact gggcagacta tctcttcaaa gacggttcga tgattggttt aaacgcggat
    14341 attctgaaga cctatgtaaa ataccgcacc aatctggcta tgaatcgtct tggcctgaag
    14401 gctttatttc cagaagttac cacagatccg ctggtctgga tgaacaagtg gttgttaacc
    14461 gacacactgc aaattgcacc acaagaggca gagcaaagca catatctggt aggtcagatc
    14521 gattctaccg tggataaggc ttctctaagc cagtttgcag acctgtaaac cgatacaaag
    14581 cattatgtgg cctggcaacg ctgggccaca atggatcaca agaattaaga aggaacaaaa
    14641 ctagtatgaa ctttaccaaa ctgactgacc acctgaaact tgccaccgat cgactcattg
    14701 gatttaagcc agaaccatat gagttgcatg aaggccatgg tgtagccact gaaagtattt
    14761 acaagatggt cgatcagttt catgaactct tccagcatcc gagacgcgtt atgccgacac
    14821 cagagctgct tcgtctccgt gcaagcctga ttcatgaaga agctgtagtg gaaggtattc
    14881 cagccgcaat gaatggggat attgagcaac tgctggatgc aatggccgac tttttatacg
    14941 ttggtgttgg tacgatggtc gccatcaaag gtggtatttc taccggcatg acctattaca
    15001 cgcaggaaca gagcattgat cgctttatgc agacaatttt tgtgcccggt aacactgttt
    15061 tcgatgatat ggcaatgcca tttcaggaag ctcgtgaggc gtcatgtatg ctcgaagagc
    15121 tggcagataa acttgagaac aagactgtta aggattctga gctgattcag gaactgcgcc
    15181 gtgtcatgaa caaaatctat gtggcgtgca tgatgaccta tcgactggct gatttcctcg
    15241 gtatcaatgt agtcgagctg gttggcgaaa ttcatcggtc taacatgaca aaattatggc
    15301 ctgctgatgt agaggaacgt cgccaggctg tggccaactg caaatacgac tcttcagacc
    15361 tgggatttcg ccatgctgat ggtaccgata agatgatcgg ttttcgaatt tccgatggaa
    15421 agattctgaa gtctccaacc tatagtgatg tcgatttatc ctcctttgtt gagcaagcta
    15481 aagcctcagc aatgtacgga atgatcaaaa aataattgta ggtagttatc tatctgtgta
    15541 tatttaacat gcgtaataaa actctggaat gactattcgt tttggtggcc tatggccacc
    15601 atttttttat ctgtctggtc ttgttctctc aatgaatgta aactcacacg atgaataagt
    15661 aattacttat ctttgtgagg tttttgtgtc actccttttg aatcgagagc atacgaacgg
    15721 tcaggtaaca aacgcatcgt atgcaaaagt tattgagacg gtgcttaaaa gcggcgtgca
    15781 ggctgatgat cgcacaggca ctggtacttt aagcacctgc tacgttccct cttactacat
    15841 gcttactggt gggactgtgc cgcttatttc tggaaaggcg gtaaatctta agccactact
    15901 tgtcgaactt gagtggtatc tgaaaggcac gggcaacatc caatttctca aggataacgg
    15961 cgttaagatt tgggatgcat gggccgatga gaatggcgat ttggggccgg tttacggtaa
    16021 gcagtggcgt cgatgggaag atacccgcat cgtgagccat agtgaatatc tgagcaagat
    16081 cgctactttc cgtgaacgcg ggtacaaagt cgagggatac ctgggtatca gtgaagatcg
    16141 cgtagtgctg tcccgtgaaa tcgatcagct acagcgtatt gtcgatacac tgcgcacgaa
    16201 ccctaccgat cgtcgcatca tgcttaacgc atggaacgta ggcgagcttg aggatatgaa
    16261 actgccacct tgccactttg tcttctcttt gtggagtcgt gagctggatt ttgaaacccg
    16321 tttaacgatg gcaactgaca ttggtcttca acacagtcgc ctcggttacg agtctatcta
    16381 caccaagatg ctatacgatc tggagatgga cggcagtgtt actgaagctg aactggatga
    16441 acttggaatc cccaaacgca tcctcaactc ctgcctcgta cagcgtagcg tagacacttt
    16501 tgttggtatg ccattcaata ttgctggcta tggcattctc actcattttc tcgcgaagat
    16561 tacgggtcac atggccggtg catttgtgca ttttggcttt gacgtgcatt tgtacaacaa
    16621 ccacatggaa ggtgtgtgtg agctaatgaa acgacaggct ccagagcatt cagatccggt
    16681 cgttattttc cctcatgaat ggtcagagtt ggatgatttc aaatgggacg aggttttaat
    16741 tcttggctat gaccctctac cgtggatcaa ggttccagtg gcggtgtgat atggcaagag
    16801 gtatgtatgt cttatgcgaa attgaaggtg tgctggcaaa tgccagccat cgtaaatcag
    16861 tatctgacgc ggatgcaggc cagctcattg ccggtgatga actcattttc cccaccagcc
    16921 gtatgttgcg tggttttgct cgctcagggg ctgaagtggt gcttatcagt agccgctctg
    16981 aaactcttga agcgccaact aaacgatggc tgaaagattt tggcgttgat tatgactggc
    17041 ttcatcttgt accgaatggc accagttatg agaagcatat taagcgcaca ttagcggagc
    17101 ataaaggcga tctgcttatc gctgcgctgg tgcacgatcc tcgactccgt gccgctttag
    17161 ccgactctca ccatcgaccg gtcatctatg aggtgagcaa atgaagatga tcgctgctgt
    17221 tggccgtaac tatgagatcg gcatagcgaa tgaactcccc tggcgttgtt ctaccgatct
    17281 gaagctattt aagagactca ccaaaaacgc cactgtcgtt atggggcgta aaacgatgga
    17341 aagtctcaaa cgccctcttc cagagcgtca taacctcgtt ttgacgcgct ctcatggctt
    17401 tgtaccaaat ggattctacc ctgctggtgt ggatgatgtg ttgcgattac cagagcctgt
    17461 gtgggtgatt ggcggggaac aaatttactc gctattcatg ccgcatgttg aagagatttg
    17521 gctctcccac atcggcgttg atgtaccaaa cgccgatgca ttcttcccgg caagcatgat
    17581 gcgtaactta ggctttgtgc ctgttgaaac agcttatacc caacgagcca gcgaggaaga
    17641 gcctggcttt tcgcagatcg tatacagaag gtcgtaatgg attaccggat tgggatcact
    17701 ggtgctcagg gcagtgggaa aacaaccctg gcaaaatata tcgacaaaca ttacggaatc
    17761 ccttacgtgg atgctggtgt cggaagtttg atgagccgcc tcggtgttcg agtaggtgag
    17821 tctatgcctc tatatgagcg gcttcagatt caaatggaaa tagcaaagca tatagagcta
    17881 cttacgcgtg gtgttgaagg ctttgttatc gatcgcacac ctgctgatgt tatggcctac
    17941 acgttggatt tggtcggcca taccaatgaa gatcgatgta ttgagttagc cctcgatatc
    18001 gaaaagtttt gccacaaaac tgctatttca aactttaacg ccattgctgg cttacgcccg
    18061 ggagtcgctc tctcagagcg agattacttg cgatcacaac gagcatcatt agaccgtctg
    18121 tatgtcgctc gtattgatgc gttgatgtgc ggggaactga caaaaattca cctgcatccg
    18181 caaagggggg atctgcaaac cttcgtcgtt tccaaccgat atcgcacggt tgaagcaaga
    18241 gccagatcag tgatgagaat gctagataac gctgtagaaa agatagaaaa ccggttctgt
    18301 ggccgagtga ccgttcatta gaaattgttc gcctcttcga cattgcgaca ataaaactct
    18361 caaaatgggt taaggataaa aaatgtttag tgaaatgttg cttgaagatg aactggatcg
    18421 gaaaacaaca gaggctttga ttcgtgtagc ggacgaacat tcccggtcgc ttatgagcga
    18481 tcgagaggct cgtctggcta ttcgtgccat attcgaaact gcgcaggggc ttgttggcgc
    18541 acaagtaggt gaagccatta acatcgccat gtctcagttc agtgaagaca gtaaaaagcc
    18601 tctgtttcct atgcatttga tgctggctgg tggcacggtg ctttatatct ctgtttgtct
    18661 ggatagcaac caaatcaata ttctcaacac tgcgtcaggt aagtggaaag atccgattgt
    18721 ctgtgaaacc agtgaagaaa ctttgaaaaa agcggctcaa tttgtacgta gcgcactact
    18781 taagggcgct aagaagttgt aaggagttct gatgacaacg attgttgcag gcatcgatat
    18841 cgagtctacg ggactggatt tccttgctgg tcataaaatt attgaaatcg caattacccg
    18901 ctatgaactg gagacacaga gacatattga tagtctggag atgcgtttta accctcgcag
    18961 aaacatagat ccgaaagctc aagccgttca tggcatttca ttagaacagc tcgcagctga
    19021 acctttgttg tcaaatcatg ccagcgaaat tggcgcttat atgggggcat gtagtgcgtg
    19081 gattgctcat aacggcgaag cgtttgatat accatttatt cgacacgagt tttcagggta
    19141 tggagtaaga ctgccagacg ttcccgttat agatactatg ttatcgggat tgtgggccac
    19201 agaagacggt aaacgtcctc gccttgaaga gttggccttc tctcttggct ttatatacga
    19261 tcatgccaaa gcacatagtg ccttatatga cacaaactta atgatgcaat gcttctttaa
    19321 ggcacgtaat aagtacggat tttttaaatt accctctgaa attgtgtaaa acaaaagcct
    19381 actttaaaga gagtttaaag taggctttct tttaaagaac agccgccttt caatcatttc
    19441 ctgcctgtat ttaatacttt tccgcctgat aggtttagtc aaaatgtagc tatcgaaacg
    19501 caaatgtaac caagcagaag gagacttaca tgagttcggt tgaaaatgta atgacaaatg
    19561 atgatctgga cgagctgaca gccatgttgc aatcacttga tgaaccagta aaaaaagccg
    19621 cacaggttga aaatactgat gatattgacg atctgctgct cggcctcgat gctggcgtag
    19681 ccatgagttc tgatgatgtt gccgaagaac tgttcaatga agaaaaagca ggtgatttca
    19741 gctctgcttt aaatgagttg gagttagcgc atgagcctat aaacgtaatt aacgctgaaa
    19801 gtgttgaatc tgccgaaaac gagccagaac aattgggatt gattgaggtt gaagagtgtg
    19861 ttgatgttaa tgatgaatta aaagttcaac agtcaaatga tagcaacaca aataaaaaag
    19921 cgcgtactgc aagaggtcct cgttttactt taagtgataa agatgattcg tttttcaata
    19981 aagcaggctt agaaacagat attttcttag acgcttacga aaacgcgcct gtcaaagcaa
    20041 aggataagat attaaacctt cttaattggt ttagcggagg tccagatatt agtgtttaca
    20101 cggtaatttc catgagacac cttctcacag aaaagaaggc tacaagtaat agtattaaga
    20161 ttgctttaat gagcaatcca gaaaaaccgt atccgcttaa cactgcgtca actcaggctg
    20221 ggcaaatgat ggctgtattt ccagcgacag gaattgccgt tagagacggt ggaaatctaa
    20281 cattgaacga agaatcaccg atcgttaaga agtttgtagc ggagtacact attggatgac
    20341 gtccccctac tgaaaataaa gcccatagag agctttatag tgctgggtaa gccaatcaca
    20401 tatccagcac cacaaaaacg cgccagagag cttctcgttt gcatttctgg cgcgttttat
    20461 ttgattgcca gacataaaat caaatgcaaa aataggtaat cacttaccta tcgagaaaga
    20521 cgatgattgc agccgaaaaa atcaaacagc gaaagcgcga caactctctt cgtgacctct
    20581 ggagaacacc tgactggctg ttttctgcca ttcaacgtta tcttggagtg acatttgatg
    20641 ttgacgttgc ctgcaacaag gacaatgcga agctgcctaa tttcataggc gttgagcgtg
    20701 atgctttgaa atctgaatgg ggacagccag gtacaattgc cttcctcaat ccaccctact
    20761 ccaaaatctc cccctggatt gatgcggcta tacgtgagca ggctcgcgga gttacaacag
    20821 tgatgctaat tcctcaatcc ctcgatacaa agtggtatga gcgtgcaaca gagtgtgcga
    20881 atgagacgat tattctgtct ggtggccgcg tagcgtttgt cgagcctgac gtcaatttgg
    20941 gtcaggtaga agtaaacatc aaccccggtg gcagtatgct cgttgttttt cgaggattct
    21001 gtcaggacgc tgggcactct ataagcaaga tccctttgga cgtcatgaaa agtctgggag
    21061 ggtatgatcc tgcgaatgtg atcaggaaaa aaagacaatc aaagaaggct gcttagtttg
    21121 ttctggcgtc tgtaattagc ctgcttctgt atatataaat aactacatat taattattaa
    21181 tatacggaag caggctgttt tatatcagag actcccagac ctgaacacca ctacagaatc
    21241 cactagaacc ccttcccaga cgctttaaaa tcgattttat gaaccacttt aaggaaacca
    21301 acatgtcata cccgactaat gtcgttgcgc tcgtagagag cgattttctg gcccaggctc
    21361 gtgaaatgat gaaagatcgt gagcaggctt tcaacttgta cgaatgggca attaagtgct
    21421 tgcatcttgg ggagcatcgc gaacttgttg aacagctttt aggtgagttg atcaacgagg
    21481 tgtttgcctt gaatgttcaa ctacatggtc gaaaaaataa tcaatcgaaa tgatagataa
    21541 gtacaaacta ttcataaagt gaattgtaag tgctaagatc tgatagtttc cagtcgtaga
    21601 ctggaggctc gacctgatgg gtgggggtaa gcgtcactgg cgtcaggttt aaaaaagctc
    21661 actaccagcg tagaaccggc accgtttagg ggttggggaa gggggaacca aagtgagcag
    21721 agacaagggt cactttatga ttgtcgagtc tggggtgttt cgagaggttg aatccagtac
    21781 tccccttcat aaagtgtggg aagatctcgg ttctggggtg ctgtcatcca taacttccca
    21841 agcctaagct ggcagtagac ttaggtcata acttttcagg ttatgaaacg accaggttgg
    21901 tgaggaaatt ttgtactcac ctccctggga gagtattacc tgaaaagaca acctctcact
    21961 tcgttcgagg tgaacttcac tcacttcgtt cgttcagttc aggtttataa aaacctgttc
    22021 tgggaagtaa tttgtttatt ttaataatta ttaacacgca cgcgtgtgcg cacgcgcgag
    22081 gaaaaaaaaa tcggcgcggc gcttgattca ggagtttata tgacgacgaa gacaccagcc
    22141 cgatcgcaag caaaaactcg caaaaatgac aaaaacaaaa attctccccg caccaattcc
    22201 acaacgcctg tcgtagagtt caatccccag cttaaaaccg tgaaaatctt cagtgatggc
    22261 tcttgcctta aaaatccggg tggcccgggc ggttacggta tagttctcca gtatggtggt
    22321 gaggaacgcg agttctcaga tggttttcat agcaccacca ataaccgcat ggagatgatg
    22381 ggggcactta tcgggctgga gcgtttgaaa tatccatgca acgttatttt gtactctgat
    22441 agccagtatc tgaaaaacgg catgacacag tggatgaaat ggtggaaacg caatggatgg
    22501 atgacttctg acaaaaaacc ggtaaagaat gttgatctgt ggaagcgtct ggatgaggcc
    22561 gcaagtcgac ataatgttcg ctggaagtgg gttaaaggtc acgccgggca tcgtgaaaat
    22621 gaaatatgtg atcgactcgc gaagatcgca gctttttcag cagcagatat gcctcacaag
    22681 aaagatattg gttttgttta taacaagtag taagtaagtg tttacctatc attttaaatc
    22741 atgtatctta tcagcgtcag gatgacaatg tgtcggtaag acacagttcc aggatggaac
    22801 gagaaagg
//
LOCUS       DAZGVQ010000520         9255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.guided.191, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000520 DAZGVQ010000000
VERSION     DAZGVQ010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.guided.191"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(142..1119)
                     /gene="epmA"
                     /locus_tag="ACHLAG_005601"
     CDS             complement(142..1119)
                     /gene="epmA"
                     /locus_tag="ACHLAG_005601"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFP0658619.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1444..3252
                     /gene="frdA"
                     /locus_tag="ACHLAG_005602"
     CDS             1444..3252
                     /gene="frdA"
                     /locus_tag="ACHLAG_005602"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFP0658620.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3245..3979
                     /gene="frdB"
                     /locus_tag="ACHLAG_005603"
     CDS             3245..3979
                     /gene="frdB"
                     /locus_tag="ACHLAG_005603"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP0658621.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            3990..4385
                     /gene="frdC"
                     /locus_tag="ACHLAG_005604"
     CDS             3990..4385
                     /gene="frdC"
                     /locus_tag="ACHLAG_005604"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP0658622.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4396..4755
                     /gene="frdD"
                     /locus_tag="ACHLAG_005605"
     CDS             4396..4755
                     /gene="frdD"
                     /locus_tag="ACHLAG_005605"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP0658623.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            4818..5951
                     /locus_tag="ACHLAG_005606"
     CDS             4818..5951
                     /locus_tag="ACHLAG_005606"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP0658624.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAKTYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            6040..6573
                     /gene="blc"
                     /locus_tag="ACHLAG_005607"
     CDS             6040..6573
                     /gene="blc"
                     /locus_tag="ACHLAG_005607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP0658625.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6570..6887)
                     /gene="sugE"
                     /locus_tag="ACHLAG_005608"
     CDS             complement(6570..6887)
                     /gene="sugE"
                     /locus_tag="ACHLAG_005608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFP0658626.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7062..7208)
                     /gene="ecnB"
                     /locus_tag="ACHLAG_005609"
     CDS             complement(7062..7208)
                     /gene="ecnB"
                     /locus_tag="ACHLAG_005609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFP0658627.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7319..7444)
                     /gene="ecnA"
                     /locus_tag="ACHLAG_005610"
     CDS             complement(7319..7444)
                     /gene="ecnA"
                     /locus_tag="ACHLAG_005610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFP0658628.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7496..8062)
                     /gene="efp"
                     /locus_tag="ACHLAG_005611"
     CDS             complement(7496..8062)
                     /gene="efp"
                     /locus_tag="ACHLAG_005611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP0658629.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8104..9132
                     /gene="epmB"
                     /locus_tag="ACHLAG_005612"
     CDS             8104..9132
                     /gene="epmB"
                     /locus_tag="ACHLAG_005612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFP0658630.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAKTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2315 a         2394 c         2397 g         2149 t
ORIGIN      
        1 aaaaccaata cagaattgat acctggatga tattaaatat ccagtcttaa attgtggact
       61 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      121 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      181 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      241 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      301 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      361 cgtcaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      421 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      481 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      541 ttgtagcagc gtgtcgcggt cttcttcggt atcagcaaca ttgctcaaat ccagtttcgc
      601 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      661 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      721 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actccagcat
      781 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
      841 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
      901 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
      961 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1021 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcgcgtttta ataagttagg
     1081 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1141 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1201 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1261 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1321 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1381 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1441 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1501 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1561 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1621 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1681 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1741 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     1801 atgaaaatcg agcgcacttg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     1861 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     1921 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     1981 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2041 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2101 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2161 tccggtatcc tgatgaccga aggctgccgc ggtgaaggcg gtattctggt caacaaaaat
     2221 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2281 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2341 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cctgcgtcac
     2401 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2461 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2521 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2581 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gttctaactc cctggcggaa
     2641 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2701 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     2761 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     2821 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     2881 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     2941 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3001 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3061 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3121 ggcacgactc gcctggagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3181 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3241 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3301 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3361 tgcgctgggc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3421 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3481 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3541 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3601 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3661 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3721 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     3781 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     3841 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     3901 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     3961 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4021 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4081 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4141 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4201 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4261 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4321 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4381 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4441 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4501 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4561 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4621 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4681 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4741 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     4801 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     4861 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     4921 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     4981 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5041 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5101 ctcgagggga aatcaagtta agcgatccca caacaaaata ctggcctgaa cttaccgcta
     5161 aacagtggaa tgggatcaca ctattacatc tcgcaaccta cactgctggc ggcctgccat
     5221 tgcaggtgcc ggatgaggtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5281 agcctgcatg ggcgccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5341 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa acacgtgtct
     5401 tccagccact caaactcaac catacgtgga ttaatgtacc gcccgcagaa gaaaagaatt
     5461 acgcctgggg atatcgcgaa ggcaaggcag ttcatgtttc gccaggggcg ttagatgcta
     5521 aaacttatgg tgtgaagtcg accattgaag atatggcctg ctgggtacga agcaatatga
     5581 atccccgtga tatcaacgac aaaacacttc agcaagggat acaactggca caatctcgct
     5641 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtaa
     5701 atcctgacag catcattaac ggcagtggca ataaaattgc actggcagca caccccgtaa
     5761 aagcgattac gcccccaact cctgcagtac gcgcatcatg ggtacataaa acaggggcaa
     5821 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atagtgatgc
     5881 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctggcag attctcaacg
     5941 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6001 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6061 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6121 gtaacagtag tgaataattt cgacgccaaa cgttatctcg gtacctggta tgagattgcc
     6181 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagcctgcgt
     6241 gatgacggcg gcctgaatgt cattaataaa ggctataacc ctgacagagg aatgtggcag
     6301 cagagtgaag ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca
     6361 ttctttggtc ctttctatgg cggttataac gttattgcac tcgatcggga ataccgccat
     6421 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6481 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6541 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6601 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6661 acaatgccgg tgatagccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6721 cctactggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     6781 acggtaataa cactcggcgt caaacggcta aagccgtggg tatatttcag gccaacggcc
     6841 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     6901 gaattggggc cgtccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     6961 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7021 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7081 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7141 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7201 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7261 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7321 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7381 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7441 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7501 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7561 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7621 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7681 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7741 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     7801 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     7861 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     7921 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     7981 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8041 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8101 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8161 gccgatgttg tgaccgatcc tgatgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8221 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8281 atcgatcgca tggagaaagg caatccggac gatcctcttt tgcgtcaggt acttacctcg
     8341 caagatgagt ttgtcgtcgc gcccggattc tccaccgacc cactggaaga acagcacagc
     8401 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8461 tgcgcggtaa attgccgtta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8521 aacaagcgta actggcagac tgctcttgag tatgttgctg ctcatccgga actggacgag
     8581 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     8641 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8701 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     8761 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     8821 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     8881 gtgaacgata acgcaaaaac gctggcaaac ctgagtaatg cgttgttcga tgccggcgta
     8941 atgccgtatt acctgcatgt gctcgataaa gtacagggcg cggcgcattt tatggtgagt
     9001 gatgacgaag cacggcagat tatgcgtgag ttgctgacac tggtgtcggg ttatctggtg
     9061 ccgaaactgg cgcgagaaat cggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9121 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9181 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9241 gtttacgccg catcc
//
LOCUS       DAZGVQ010000521         5812 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.guided.247, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000521 DAZGVQ010000000
VERSION     DAZGVQ010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.guided.247"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..101
                     /locus_tag="ACHLAG_005613"
     CDS             <1..101
                     /locus_tag="ACHLAG_005613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP0658631.1"
                     /translation="WDERRGGRGVRLVGLHVTLLDPQMERQLVLGL"
     gene            98..550
                     /locus_tag="ACHLAG_005614"
     CDS             98..550
                     /locus_tag="ACHLAG_005614"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP0658632.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            796..1905
                     /locus_tag="ACHLAG_005615"
     CDS             796..1905
                     /locus_tag="ACHLAG_005615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFP0658633.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALAFACINRQMIALRIMQQVK
                     ATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDYSWIVQPVANE
                     KTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            1902..2516
                     /gene="prfH"
                     /locus_tag="ACHLAG_005616"
     CDS             1902..2516
                     /gene="prfH"
                     /locus_tag="ACHLAG_005616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFP0658634.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GHYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(2573..4030)
                     /gene="pepD"
                     /locus_tag="ACHLAG_005617"
     CDS             complement(2573..4030)
                     /gene="pepD"
                     /locus_tag="ACHLAG_005617"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP0658635.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            4291..4749
                     /gene="gpt"
                     /locus_tag="ACHLAG_005618"
     CDS             4291..4749
                     /gene="gpt"
                     /locus_tag="ACHLAG_005618"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP0658636.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4841..>5812
                     /gene="frsA"
                     /locus_tag="ACHLAG_005619"
     CDS             4841..>5812
                     /gene="frsA"
                     /locus_tag="ACHLAG_005619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP0658637.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASR"
BASE COUNT         1396 a         1428 c         1529 g         1459 t
ORIGIN      
        1 cctgggatga acgccgcggc gggcgcggtg tgcgactggt ggggctgcat gtgacgttgc
       61 ttgacccgca aatggaaaga caactggtgc tgggattatg aataacatac aaataagaga
      121 ctatcagcct ggcgattttc agcaactatg cgctattttc attagagcgg ttacgatgac
      181 tgccagtcag cattattcac cacaacaaat tgccgcctgg gcgcagattg acgaatctcg
      241 ctggaaggag aaactcgcga aatcacaagt gcgggttgca gtcattaatg caaaactggt
      301 cggctttatt acctgcgttg aacactatat cgatatgtta tttgttgacc ctgaatacac
      361 ccgccgtggg gttgccagcg ctttgttaaa acctttgatt aagtctgaat ccgaacttac
      421 ggtggacgca agcataaccg caaaaccctt ttttgaacgt tatggttttc agacagttaa
      481 gcagcaacgc gttgaatgtc ggggagagtg gtttattaat ttttatatgc gttataaaca
      541 gcaacattaa atccagcttg caatgaaaat aacgcaagcc tagtatgtgc gcgttttctt
      601 attccacaaa ctgcaaggag gtaaatcatg acaaatcctt tatcaatgac tctttgcaga
      661 cctttccagg attaattctt tttttcttgc cctggattcg tctgccattt cctgattttt
      721 taattgttac tctcaataat tcaagtcgca acacatgcaa cttgaagtat gacgagtcta
      781 tttacggaat ggtatatggg caattatatt cgtcccttat ccgatgcggt atttaccatc
      841 gcatctgatg acctgtggat cgagagttta gcgatccaac aattacacac cacggcaaat
      901 ttacccaaca tgcagcgcgt agtggggatg ccagatttac atcccggacg cggctatccg
      961 attggcgcag cgttcttctc tgttggtcgt ttttatccgg cactggtcgg caatgatatc
     1021 ggctgcggta tggcgctatg gcaaacagat attctcactc gcaaatacaa cgccgataag
     1081 tttgaaaagc gattatcaga tctggatgac gttgctgaag aaagctggct ggaggaaaac
     1141 ctgccgtcag catttgcaca gcatccgtgg cgcagctcgc ttggctctat cggtggcggc
     1201 aatcatttcg ctgaactgca acaagttgat caaatcatca acgctgaact gtttgcactg
     1261 gcaggtctgg atgcacaaca tctgcaactg ttggttcata gcggctcgcg gggggtgggg
     1321 cagtctattt tacagcggca tattgcctcg ttctcgcatc atggtttgcc tcaaggtagt
     1381 gacgacgcgc tggcgtttgc gtgtattaat cgccagatga tcgctttgcg cataatgcaa
     1441 caagttaagg ccacgggaag tccggttctg gatgtggcgc ataactttgt tagcgcgtgt
     1501 caaatcggcg atcaacaggg ctggttgcat cgtaaaggtg ccacgccgga tgacaacggt
     1561 ctggtgatta ttcccggttc acgtggtgat tactcctgga ttgtacagcc tgttgcgaac
     1621 gaaaaaacgc tccactcgct ggcgcatggg gcagggcgta aatgggggcg caccgagtgt
     1681 aaagggcgtc tggcagcgaa atacacggcg acgcaactct cgcgtactga acttggcagt
     1741 cgggtaattt gtcgcgataa acaactcatc tttgaagaag cgccacaagc ctataaatcg
     1801 gctgaaagcg tggtgcaatg tctggtgcag gccggtttaa ttattcctgt cgcgcggctg
     1861 cgtccggtgc taacgctcaa aaacagtgga gggaaaaaag gatgatcttg ctacaactct
     1921 cctcagcgca ggggccggaa gaatgttgtc tcgcagtgaa aaaggcgctg gacaggctaa
     1981 ttaaagaagc tgcccgacaa gacgtcgcgg taacggtgct ggaaacagaa acgggccact
     2041 actctgacac gctgcgttcg gcgctgattt ctctggatgg cgataacgca tgggcattaa
     2101 gcgaaagctg gtgtggcact attcagtgga tttgtccgag tccgtatcgg cctcatcatg
     2161 ggcgcaaaaa ctggtttctg ggcattgggc gttttaccgc tgatgagcag gaacaatcgg
     2221 atgcaatccg ttatgagacg ttgcgttcgt cggggccggg cggtcaacat gtcaataaaa
     2281 ccgactcggc ggtacgcgcc acgcatttgg catccggtat tagcgtgaag gttcagtcag
     2341 agcgtagtca gcatgctaac aagcggctgg cacgattgct gattgcctgg aagctggagc
     2401 agcagcaaca ggaaaatagc gcggtgctga aatcgcagcg gcgaatgttc catcaccaga
     2461 ttgaacgtgg caacccgaga cggacattta cagggatggc ttttatcgaa ggataatgaa
     2521 ggatgaaact gccggaaggc gattaaacgc catccggcag caaatcaaat aattacttcg
     2581 ccggaatttc tttcagcagt tcagtcagca gtgtccagta atgacctacg ctttcgatgt
     2641 gaacttgctc atccggagag tgtggaccgg tgatagttgg cccgatagaa accatgtcca
     2701 tttccggata cggttttttg aacagaccac attccaggcc cgcgtggata atctggatgt
     2761 tcggcgtttt gttgaacaga cgctgatagg tttcacgtac cagatgcatc accggagaat
     2821 tagcgtccgg ctgccagcca ggatatgcgc ctttcgcttc ggttttcgcg ccagccagtt
     2881 tacccagcga atccagcatg ctcaccacgt agtctttacc gctgtcgatc agtgaacgga
     2941 tcaggcagtg aatttctacg ttattgtcag tcatggtcac cacaccgacg ttcagggagg
     3001 tttcgaccac accttttgcc acgtcggaat tgcggatcac accgttcggg gtggcgttca
     3061 gcagacgaat aaaggtatcg cgagatttcg caatcagggc agctttatcg ttcgctacag
     3121 agtccagcaa caaggccaga tttttctctt tctctgccag ctcgtttttc aggatctcct
     3181 gataggtatt caccagagat ttcaggacgt cgactttatc agctgcgaca gcaatggtcg
     3241 caaaggcttc acgcgggatg gcgttacgca gtgtgccgcc gttgaaatcg ataaggcgca
     3301 gatccagttc ttccgcatga cccgccagga agcgcaccag tagtttgttg gcattaccca
     3361 ggccaacgtg gatttcaccg ccggagtgac cgcctttcag accttttaag gttaacttga
     3421 aggtttcaaa accagctgga accgcttcac gatctaaatg caggttggag gtgaagtcga
     3481 taccccccgc acaacccatg tagatttcac cttcttcttc ggagtcggtg ttaatcagaa
     3541 tatcagcctg caaccagttg ctctgtaagc cgaacgcacc gtccataccg gcttcttcgg
     3601 tcatggtcag cagcacttcc agcgggccgt gaaccacgtt ttcgtcagcc agaaccgcca
     3661 gcgcagacgc cataccaatg ccgttatctg cacccagcgt ggtgccgcgc gctttaaccc
     3721 attcgccatc aatataaggc tggataggat ctttcgtgaa gtcatgcacg gtgtcgttat
     3781 ttttctgcgg caccatatcg aggtgggcct gtaagacgac cggtttacga ttttccatac
     3841 ctgcggtagc aggtttacga atcaggatat tacctacctg atcgcgttcg acatggaaac
     3901 ctttctcttt tgcccaacca acaatgtatt cagcgagttg ctcttcatga taggacgggt
     3961 gaggaataga acagattttg gcaaaaatat cccacagcgg ctgtggagat aattgagaca
     4021 gttcagacac gttaagtctc cttgtcgatc acccgcaaaa cagtattgca ggtcacaggg
     4081 ttagcagaaa atgttgtcaa cacaagacag gcttgcgaga tatgtttgag aataccactt
     4141 tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt
     4201 tttagtgcgc cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa
     4261 gccgtagata aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac
     4321 atgttgcaga tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa
     4381 ggcattattg ccgtaagccg tggcggtctg gtaccgggtg cgttactggc gcgtgaactg
     4441 ggtattcgtc atgtcgatac cgtttgtatt tccagctacg atcacgacaa ccagcgcgag
     4501 cttaaagtgc taaaacgcgc agaaggcgat ggcgaaggct tcatcgttat tgatgacctg
     4561 gtggataccg gtggtactgc ggttgcgatt cgtgaaatgt atccaaaagc acattttgtg
     4621 actatcttcg caaaaccggc tggtcgtccg ctggttgatg actatgttgt tgatatcccg
     4681 caagatacct ggatcgaaca gccgtgggat atgggcgtcg tattcgtccc gccaatctcc
     4741 ggtcgctaat cttttcaacg cctggcactg ccgggcgttg ttctttttaa cttcaggcgg
     4801 gttacaatag tttccagtaa gtattctgga ggctgcatcc atgacacagg caaacctgag
     4861 cgaaaccctg ttcaaacccc gctttaaaca tcctgaaacc tcgacgctag tccgccgctt
     4921 taatcacggc gcacaaccgc ctgtgcagtc ggctcttgat ggtaaaacca tccctcactg
     4981 gtatcgcatg attaaccgtc tgatgtggat ctggcgcggc attgacccac gtgaaatcct
     5041 cgacgtccag gcacgtattg tgatgagcga tgctgaacgt accgatgatg atttatacga
     5101 taccgtgatt ggctaccgtg gtggcaactg gatttatgag tgggccaccc aggcgatggt
     5161 gtggcaacaa aaagcctgtg cggaagaaga tccgcaactc agtggtcgac actggctgca
     5221 tgcggctacg ttgtacaaca ttgccgccta tcctcatctg aaaggagatg acctggccga
     5281 gcaagcgcag gctttatcaa accgcgccta tgaagaggcc gcccagcgtc tgccgggcac
     5341 gatgcggcag atggagttta ccgtacccgg cggtgcgccc atcaccggct ttttgcatat
     5401 gccgaaaggc gatggcccgt tcccgacagt attaatgtgt ggtggtctgg atgcgatgca
     5461 gacggactat tacagcctgt atgaacgtta ttttgcgccg cgcggcattg cgatgctgac
     5521 tattgatatg ccgtcggtgg gcttttcttc aaaatggaag ctcacccagg actccagcct
     5581 gttgcatcag cacgtcttaa aggcgctgcc taacgtaccg tgggtggatc acactcgcgt
     5641 cgcggccttt ggtttccgtt tcggcgctaa cgttgccgtg cgtctggcat accttgaatc
     5701 gccgcgtctg aaagcggttg cctgtcttgg tccggtagtt cataccctgt tgagtgattt
     5761 taagtgccag caacaggtgc cggaaatgta tcttgacgtt ctggcgagtc gt
//
LOCUS       DAZGVQ010000522         1684 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2110S26431
            SAMN43267688-rid23077343.guided.343, whole genome shotgun sequence.
ACCESSION   DAZGVQ010000522 DAZGVQ010000000
VERSION     DAZGVQ010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267688
            Sequence Read Archive: SRR30307323
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350641.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350641.1
            Genome Coverage       :: 85x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:57:07
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,621
            CDSs (total)                      :: 5,538
            Genes (coding)                    :: 5,325
            CDSs (with protein)               :: 5,325
            Genes (RNA)                       :: 83
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 72
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 213
            CDSs (without protein)            :: 213
            Pseudo Genes (ambiguous residues) :: 0 of 213
            Pseudo Genes (frameshifted)       :: 87 of 213
            Pseudo Genes (incomplete)         :: 111 of 213
            Pseudo Genes (internal stop)      :: 50 of 213
            Pseudo Genes (multiple problems)  :: 31 of 213
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267688-rid23077343.guided.343"
                     /serotype="O 91:H14"
                     /isolate="2110S26431"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-10"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            311..1258
                     /gene="stxA1a"
                     /locus_tag="ACHLAG_005620"
     CDS             311..1258
                     /gene="stxA1a"
                     /locus_tag="ACHLAG_005620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFP0658638.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            1268..1537
                     /gene="stxB1a"
                     /locus_tag="ACHLAG_005621"
     CDS             1268..1537
                     /gene="stxB1a"
                     /locus_tag="ACHLAG_005621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFP0658639.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          451 a          290 c          408 g          535 t
ORIGIN      
        1 ttgcaggatg accctgtaac gaagtttgcg taacagcatt ttgctctacg agtttgccag
       61 cccccccagt ggctggcttt tttatgtccg taacatcctg tgtatcaata aatgttgttg
      121 tctacgtacg tcaagtagtc gcatgagatc tgaccagata tgttaaggtt gcagctctct
      181 ttgaatataa ttatcatttt cattacgtta ttgttacgtt tatccggtgc gccgtaaaac
      241 gccgtccttc agggggtgga ggatgtcaag aatatagtta tcgtatggtg ctcaaggagt
      301 attgtgtaat atgaaaataa ttatttttag agtgctaact tttttctttg ttatcttttc
      361 agttaatgtg gttgcgaagg aatttacctt agacttctcg actgcaaaga cgtatgtaga
      421 ttcgctgaat gtcattcgct ctgcaatagg tactccatta cagactattt catcaggagg
      481 tacgtcttta ctgatgattg atagtggcac aggggataat ttgtttgcag ttgatgtcag
      541 agggatagat ccagaggaag ggcggtttaa taatctacgg cttattgttg aacgaaataa
      601 tttatatgtg acaggatttg ttaacaggac aaataatgtt ttttatcgct ttgctgattt
      661 ttcacatgtt acctttccag gtacaacagc ggttacattg tctggtgaca gtagctatac
      721 cacgttacag cgtgttgcag ggatcagtcg tacggggatg cagataaatc gccattcgtt
      781 gactacttct tatctggatt taatgtcgca tagtggaacc tcactgacgc agtctgtggc
      841 aagagcgatg ttacggtttg ttactgtgac agctgaagct ttacgttttc ggcaaataca
      901 gaggggattt cgtacaacac tggatgatct cagtgggcgt tcttatgtaa tgactgctga
      961 agatgttgat cttacattga actggggaag gttgagtagt gtcctgcctg actatcatgg
     1021 acaagactct gttcgtgtag gaagaatttc ttttggaagc attaatgcaa ttctgggaag
     1081 cgtggcatta atactgaatt gtcatcatca tgcatcgcga gttgccagaa tggcatctga
     1141 tgagtttcct tctatgtgtc cggcagatgg aagagtccgt gggattacgc acaataaaat
     1201 attgtgggat tcatccactc tgggggcaat tctgatgcgc agaactatta gcagttgagg
     1261 gggtaaaatg aaaaaaacat tattaatagc tgcatcgctt tcattttttt cagcaagtgc
     1321 gctggcgacg cctgattgtg taactggaaa ggtggagtat acaaaatata atgatgacga
     1381 tacctttaca gttaaagtgg gtgataaaga attatttacc aacagatgga atcttcagtc
     1441 tcttcttctc agtgcgcaaa ttacggggat gactgtaacc attaaaacta atgcctgtca
     1501 taatggaggg ggattcagcg aagttatttt tcgttgactc agaatagctc agtgaaaata
     1561 gcaggcggag attcataaat gttaaataca tctcaattca gtcagttgtt gccggtctga
     1621 taatagatgt gttagaaaat ttctgcatgg tgaatccccc tgtgcggagg ggcgactggt
     1681 gaac
//
